LOCUS       DBGJFF010000001       124640 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.001, whole genome shotgun sequence.
ACCESSION   DBGJFF010000001 DBGJFF010000000
VERSION     DBGJFF010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 124640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 124640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..124640
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.001"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            535..675
                     /locus_tag="ACOIAF_000001"
     CDS             535..675
                     /locus_tag="ACOIAF_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667038.1"
                     /translation="MSEGKINKEKLMKLGNMLSELKRNNKDKFTNYKSEVEKYENKKR
                     GA"
     gene            complement(686..1024)
                     /locus_tag="ACOIAF_000002"
     CDS             complement(686..1024)
                     /locus_tag="ACOIAF_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGZ2667039.1"
                     /translation="MNKRLKTLRKELGLNQTQMGNKLFLSQDHISSLETGRRDLTDRI
                     INDICKEFNVNENWLRTGEGDIFQDPTLDMDFDDDIKEMLRMISKLSPEAQKRLYNVA
                     EVFLEEENKK"
     gene            1187..1342
                     /locus_tag="ACOIAF_000003"
     CDS             1187..1342
                     /locus_tag="ACOIAF_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667040.1"
                     /translation="MHKKKMNQKELELLGKMFLKIKKIDKQKFGELKGSVKTSLEYEY
                     ILVNKIR"
     gene            complement(1498..1830)
                     /locus_tag="ACOIAF_000004"
     CDS             complement(1498..1830)
                     /locus_tag="ACOIAF_000004"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGZ2667041.1"
                     /translation="MYTRVKQIREKLKLNQAEFAKKIGLSQSTLAMIEVNKRTFSDKH
                     IKLICSEFGVSENWLRTGEGDMFASSPYEKEFLNIFDSLTSDTKEYLLLMAKELLKTQ
                     NKLLNSKK"
     gene            1994..2137
                     /locus_tag="ACOIAF_000005"
     CDS             1994..2137
                     /locus_tag="ACOIAF_000005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667042.1"
                     /translation="MEDIITTEMKNTFLNLSVNNQLEILNLAKTIKKIEDALNIEDNE
                     NYN"
     gene            2168..2962
                     /locus_tag="ACOIAF_000006"
     CDS             2168..2962
                     /locus_tag="ACOIAF_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HGZ2667043.1"
                     /translation="MNNLRIKGKQKFIDLEIPIIEGGFGESKKCMTDKTISEIHNMRN
                     ADIRRRISDNLKRFKNNIDYIDLKRVHETHTLIELGYSKQSITQADNIFLLSERGYSK
                     LIKIMDTDLAWEIHDKIMDEYFNMREQLTDSYMIQDSIERAKRWIEEEKERLLLKETI
                     QVQQPKVEYHDKVLNPDKLVTTTDIAKDLGMTAQALNKLLHLNRIIYPERINGRIKCW
                     KPYKNYLWLIPEYADFKITEHDTQLKWTEIGRKWLIEFVENIKKIS"
     gene            3026..3250
                     /locus_tag="ACOIAF_000007"
     CDS             3026..3250
                     /locus_tag="ACOIAF_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667044.1"
                     /translation="MIKQILNKRGSLLNLASEYLTEQDYLKFQGWYTHRYSKLDAYDA
                     SINEYLNYKNDKKLITYVFNLKFQEALIEI"
     gene            3274..3813
                     /locus_tag="ACOIAF_000008"
     CDS             3274..3813
                     /locus_tag="ACOIAF_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667045.1"
                     /translation="MNYNYVTKIDLKYGNTQFGFEKYSDALDKYRELKEEFKYVPCII
                     KLHSYVAHQDLYVKEIKADGFEKKYLDIKNLIKLFKEHKQNISDELSLCDKKKEEVLH
                     VIEEMNLDTASLNDKIDTIKELKNTLVKRRLVKYELNKYFAFCNTLYEIEEELKKYES
                     KYKQKENQGKSRFKKIKNR"
     gene            3818..3958
                     /locus_tag="ACOIAF_000009"
     CDS             3818..3958
                     /locus_tag="ACOIAF_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667046.1"
                     /translation="MEEYHFNLIKKLCAERKIHKGWFRNIEKCGCGGKCNKKNCKMLE
                     KL"
     gene            3969..4202
                     /locus_tag="ACOIAF_000010"
     CDS             3969..4202
                     /locus_tag="ACOIAF_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667047.1"
                     /translation="MENQTELTEKDIEKTLSALNIIKYICIISKHCKNCPLGYMKNLD
                     EHNCMLYELVDKGLLPCDWSVKKVTRLLDTTEE"
     gene            4221..4508
                     /locus_tag="ACOIAF_000011"
     CDS             4221..4508
                     /locus_tag="ACOIAF_000011"
                     /inference="COORDINATES: protein motif:HMM:NF021182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HGZ2667048.1"
                     /translation="MELDFRIWNGFRMIHGEELRLLTEEMFIKSGYSEQYYNDICIPR
                     VEDLKIMQYIGLKDCFEKKIFQYDIVKFIFDGVWKVCLVNLSLPKRSNSYI"
     gene            4645..4839
                     /locus_tag="ACOIAF_000012"
     CDS             4645..4839
                     /locus_tag="ACOIAF_000012"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667049.1"
                     /translation="MDEMIIRVKMGEPLPTLGKIIKCTTCLTNEEKCFKVNKICKLSW
                     INDRNSEGLIVECKGKYIKY"
     gene            5046..5600
                     /locus_tag="ACOIAF_000013"
     CDS             5046..5600
                     /locus_tag="ACOIAF_000013"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003950 - NAD+ ADP-ribosyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HGZ2667050.1"
                     /translation="MSKKDKLSVFISLILRHKPEIIGIKLDDYGYADVNELIEKINNT
                     GRNINIEILEQIVKEDNKQRYSFNDDRSKIRANQGHSINVNVELRELEPPRFLYHGTA
                     TRFLDNIKKEGIVSKSRLYVHLSNDIDTAVQVGKRHGVPVVLKINTGKMYENGYKFYL
                     SENNIWLCKYIPFEYVEKISRVVK"
     gene            5627..5953
                     /locus_tag="ACOIAF_000014"
     CDS             5627..5953
                     /locus_tag="ACOIAF_000014"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667051.1"
                     /translation="MDFNFRKICEKDELTDDFIIKICQLSHEEFGGTIYVPCNIHPCG
                     WDELEVPNPKDLDSDKLMEEFLLEYKFECENISSNIYDSDHPFIGKTLREILMIVDCR
                     FIIGVI"
     gene            5968..6744
                     /locus_tag="ACOIAF_000015"
     CDS             5968..6744
                     /locus_tag="ACOIAF_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667052.1"
                     /translation="MENIELTPRDYFEDIKNKKQKNTNENLDIVYENSLILLNKYIIT
                     GQKKGAKKLMFLLECIEKERSVIELGIDTFVYKEDIEKYIDDISKNTVKIIELENYER
                     EIPDEIVEIIEKTKDIFDKMYVLFTDYTGKITKKISKERRDKDPILFGVFSDEKSRAI
                     VDRFYYLGDWEDEYCDLTLEKMINDYKKKGKQDISKHIITPKSLDELKKELDSLEKNN
                     DTYKQIINIKPLPTPSNSSVEPPKSNIFKKIKNRVFKNEK"
     gene            6734..7138
                     /locus_tag="ACOIAF_000016"
     CDS             6734..7138
                     /locus_tag="ACOIAF_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667053.1"
                     /translation="MKNNVDLTENRDFRDVRGKGLSILGIEETPNRMLNGFSDFGIRH
                     LYGKNLEFEFDKNEIFLTGNANDREEKKFFMLCEENKVCIRCGDELKEGWDKVSIELC
                     NKCYEMLDVEYKNKKIIRELFFRDRDVNIELI"
     gene            7176..7565
                     /locus_tag="ACOIAF_000017"
     CDS             7176..7565
                     /locus_tag="ACOIAF_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667054.1"
                     /translation="MKDVWLYSWDDECFRSDEYESKEEAVEAAKEELKRLGEIKRWAY
                     IGKKEEVDIPDIDAEDALEFVQDRIDNEFGGYGEDWFENIRAEDIVILENRISKVFKK
                     WIDEFGYKPYWFVVRDTEEIELKNAVN"
     gene            7625..7945
                     /locus_tag="ACOIAF_000018"
     CDS             7625..7945
                     /locus_tag="ACOIAF_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667055.1"
                     /translation="MFIKGFRCIFRHKFSRIKDCQLITTRSQQYTCKIWMCKYCGQIV
                     AGSTPVYHPNMKVNEYCIKCEFSACANNYEGYCNSKDKEIEFKSICLEDNKEILQCKN
                     LDLE"
     gene            8053..8124
                     /locus_tag="ACOIAF_000019"
     CDS             8053..8124
                     /locus_tag="ACOIAF_000019"
                     /inference="COORDINATES: protein motif:HMM:NF019481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667056.1"
                     /translation="MFLSKVDKEKYPSVIQEYRFEEV"
     gene            8162..9178
                     /locus_tag="ACOIAF_000020"
     CDS             8162..9178
                     /locus_tag="ACOIAF_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HGZ2667057.1"
                     /translation="MILDIKSRLESKEYNFLREEERLKNRMILLTTGGSFAYGTNIDT
                     PEHISDLDIRGIYLNSAQEILTMNCINKPYENKQTDTVIYPLKQIITLLLNVNPNVIE
                     ILGTRKEDLFVCTEEGKLLKDNVDLFLTKRAANSFGGYAISQLRRLQNALARDNYKQK
                     EKEEHIMNSVKRQMKTFEDRYKIVSEEEIKLYIEESKRTDLEEEIFIDVNLKKYPLRD
                     LKGMWSEMNNILKDYSKLNHRNNKKDELHLNKHAMHLIRLFLMGIEILEGKGINTYRE
                     NDKEFLLDIRKGKYTYNEIFEMVNELDKNFKYAEKNSCLPNSPDMKKVNELVMYMTNR
                     VLNR"
     gene            9319..9669
                     /locus_tag="ACOIAF_000021"
     CDS             9319..9669
                     /locus_tag="ACOIAF_000021"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667058.1"
                     /translation="MNINKLKKIKERKIKRWNIEKITNRINDHMNIQEDIEKWDKFET
                     PIISKEVDFYLLKIGFQYGRNNGRNVLVGIKDMRHISISKVNSNLLSIDIYKFIKQED
                     GKFSEFKAHEYIEK"
     gene            9723..10124
                     /locus_tag="ACOIAF_000022"
     CDS             9723..10124
                     /locus_tag="ACOIAF_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HGZ2667059.1"
                     /translation="MKELLEVKKIFDSLASTSSRKEKERILEKYKNNRMFVECLQFLL
                     DSNILTGISKNKICKNLNNTSHNELENIYDMLDYLIKNNTGRNIDVKTIQVFASKDEK
                     LKDFIFNLATKSIKLGITYKTVDKIMPGLII"
     gene            10226..10729
                     /locus_tag="ACOIAF_000023"
     CDS             10226..10729
                     /locus_tag="ACOIAF_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667060.1"
                     /translation="MTDKIIIASVDGEEMIFRTEMKKLEKGDSLIVSVNGVKRLALFI
                     RYISTCEEYIEDIAFQKVDDILYENTIAKEKERVKKVFKDKCMKNYKPIVNTEIDSYL
                     RECGFNCDGRQFFKSIDNTQILISRDCDKDVLTVSKGLTITKTDITNKNLITVIKIIN
                     NSLLIVR"
     gene            10789..13014
                     /locus_tag="ACOIAF_000024"
     CDS             10789..13014
                     /locus_tag="ACOIAF_000024"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside triphosphate reductase"
                     /protein_id="HGZ2667061.1"
                     /translation="MKNVVKRDGRIVPFDEKKIINAIEKSFLSLRNKGKSEVFDRELA
                     TLIAKKIKNKPCEKMHIEDIQDIIEFSLMASKKKDVAKEYITYREKRNIARGRKTYND
                     YMSIVNTESNDITKENANMNADTPAGMMMKFASETTKTFTDHVLLSEEAKEAMMDNYI
                     HIHDKDYYPTKSLTCIQHPLDKIFNNGFRAGHGSSRPTKRIETSSILGCISLETVQNE
                     MHGGQAIPAFDFYNAQSVRKTFIEEIKNIEIKDDIDLSHLYNIEIEDYIIKDLKELDS
                     EKRNVQHAINRTVWRVHQSMESFIHNMNTIHSRGGNQVVFSSINYGTDTSAEGRCIIR
                     EILKSTYEGVGNGETPIFPIQIWKVKEGVNYNPTDRNYDLYELACKVSAKRFFPNFLN
                     LDATFNKHVKWKMCDPNRYIYEVATMGCRTRVFEDLHGEKTSIGRGNLSFTTLNLVKM
                     ALEAKEKYPNNEANRVAEFIFLLDKYSDVVARQLYDRYLFQCTAKAKQFPLLMSGLWK
                     NSENLNPNDDVSEVLKHGTLGVGFIGLAECLIALIGEHHGESEKAQKLGLEIISLLKS
                     KVEKYKKKYNLNYSVLGTPAEGLSGKFTQKDKKIFGEIKNITDKDYYTNSSHVPVWYN
                     CNVEHKAKIEAPYHELEGGGHIFYVELDGDATHNPKAIMKVVDLMKKYNMGYGSVNHT
                     RSRCMNCGFENADSELKECPKCGSNSINIIQRITGYLVGTTDSWNNAKLAELKDRVTH
                     G"
     gene            13007..13504
                     /gene="nrdG"
                     /locus_tag="ACOIAF_000025"
     CDS             13007..13504
                     /gene="nrdG"
                     /locus_tag="ACOIAF_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HGZ2667062.1"
                     /translation="MDKDYIYILDIKHDTIVDGEGFRTSIYCSGCNHMCRGCHNPQSW
                     DIKNGTLTKVLDIYKEIISNKFSDVTFSGGDPLLQLQGFVHLAKLIKKNTNKTIWCYT
                     GFKFEELITDNEKLELLKLIDVLVDGKFEEDKKSLELIFKGSSNQRIIDVQKTLYEKQ
                     IVLYE"
     gene            13539..13670
                     /locus_tag="ACOIAF_000026"
     CDS             13539..13670
                     /locus_tag="ACOIAF_000026"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667063.1"
                     /translation="MTKEEFITKFLKEQQRRIKVLYYEVENFKMDRRFNERKKSRRH"
     gene            13705..14112
                     /locus_tag="ACOIAF_000027"
     CDS             13705..14112
                     /locus_tag="ACOIAF_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667064.1"
                     /translation="MYFETDIIKNKNTVNKIIKYCGGNTEKLKDWLKEIENKSISSLE
                     ELKEYPLKRNYKFVFSKEEAPNLDKLIKMKLRDNCLEKCEICSLENLLSMLNDEILDL
                     NKEDISYEDNIEWIENCKEELIKLKVGSICIDW"
     gene            14133..14669
                     /locus_tag="ACOIAF_000028"
     CDS             14133..14669
                     /locus_tag="ACOIAF_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667065.1"
                     /translation="MRFWCENCNKYFNAEETLQEYNYFLNEEILICPSCKKDLIPIAR
                     ETELSLGFDSDTNQLTYVEYDSSDYSLLRKVNADVEDVVKPIIHYIRNLNKNSLDLNG
                     ITITMNGNREGKRLDGLNYEEGVVMDNLINAWNGFCKLKRQHPSELGDFQNAIHQAQQ
                     VLGLRVLRNDYPKGWIKK"
     gene            14690..15088
                     /locus_tag="ACOIAF_000029"
     CDS             14690..15088
                     /locus_tag="ACOIAF_000029"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667066.1"
                     /translation="MSTKTILKNIDIKDDKAAERLISALEESENKQDSDLDIKKIFEL
                     IDNSISSYIIYGTPKALSKFESILNIFKIDKANSQHVQKFIKVPAIDLGKYCDYKNNK
                     ALFDDNTVYIIPKDTKLKIYKDEYKYVVLK"
     gene            15118..15288
                     /locus_tag="ACOIAF_000030"
     CDS             15118..15288
                     /locus_tag="ACOIAF_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667067.1"
                     /translation="MTKYKIEDRTNLIIHQSKKHNITFTLILENEDKREMLKKVLRCL
                     KKERIVFLLKVN"
     gene            15426..17468
                     /locus_tag="ACOIAF_000031"
     CDS             15426..17468
                     /locus_tag="ACOIAF_000031"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667068.1"
                     /translation="MEFNNFKKQVQERFNKMTTGISYIYEVDLDKDRLWNLYLDSFPE
                     GTNEIFRERREYDCSCCRQFIKNIGNVVTIKDNKISSIWDIDTDCPTFKPVVKALSEY
                     VKSHNINDIYVSKFKKIGTDKNFENTSEKVYEWNHLYIELPDRFVDETNKSVGDIKGR
                     IRDVKNVFKRSLDEISEDSVSIVLDLINQKSLYKGEEWQNPLIEFLKYKRNYIKLQTE
                     TDKQNFAWEQSVKAGVVIGKIRNHSIGTLLLDISEGVELDKAVSRYEKIVAPSNYKRP
                     KAIYTKKMLDEAKKTITELGYMDSLQRRHATIDDITVNNILFVNKDSAKQMGEFDIFD
                     EMSKGIGIDSKKFSKVEELNIEEFIQNVLPTSKEVELLLENRHIDNFVSLIAPVNKEA
                     KSMLKWDNNFGWAYAGNMTDSSMKENVKNAGGKVDGVLRFSIQWNDTEFDNNDLDAHC
                     IEPNGNHIYFGNKTNYSTTGMLDIDIMHPKRGNVAVENITWSDINKMRLGTYKFYVNN
                     YSKRSGISGFRAEIEFNGEIYSFDYNKPLRDGENVMVAEVTLDIGNRFKIKPLLPSNV
                     SNKEIWGLTTNQFIPVSVIMNSPNYWDGQQGIGNKHYFFMLKDCINEETVNGFYNEYL
                     KNDLNVHRKVFEALGSKMYVPFSENQMSGLGFSSTRRNEVIVKVRGSYERILKIKF"
     gene            17493..17873
                     /locus_tag="ACOIAF_000032"
     CDS             17493..17873
                     /locus_tag="ACOIAF_000032"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667069.1"
                     /translation="MENVFEMATRNKFRFEFRGLISVEDLWDLKLEQLDEVYKTLTTD
                     MKKANEESLLKIKTKDQKEVELKIKIVKYIVELRLAEIENRNKEKKKKEQKQHILELI
                     NKKENQELENKSIEELKSMINSLE"
     gene            17951..18172
                     /locus_tag="ACOIAF_000033"
     CDS             17951..18172
                     /locus_tag="ACOIAF_000033"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667070.1"
                     /translation="MDSKIEVGSIWGLEDGIAQPQYVGTKVKVVSISYIEEFNKRLIT
                     FMEIEEKEEDTWIDADWEDVFLNDFFKLD"
     gene            18286..18441
                     /locus_tag="ACOIAF_000034"
     CDS             18286..18441
                     /locus_tag="ACOIAF_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667071.1"
                     /translation="MYSKGNVVKVWCYQCEKIEYQEFVGYFNCYFDNYKLKKRWKCSK
                     CGVINNF"
     gene            18468..18914
                     /locus_tag="ACOIAF_000035"
     CDS             18468..18914
                     /locus_tag="ACOIAF_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667072.1"
                     /translation="MKIKKIILGLDNLENVELGIGDVGAFYLDNIHEIIGGFTLSYID
                     KCKQADYLHIQIHKRVDEIQRLHDYKDITNVKIEYEDGSVEDIYVPYKQEGDFLGLDN
                     LYQTSFISKQGYLHIVISKDKTVEEVFNEEFEDDEGVEFSFNMYGI"
     gene            19192..19356
                     /locus_tag="ACOIAF_000036"
     CDS             19192..19356
                     /locus_tag="ACOIAF_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667073.1"
                     /translation="MTDKEMEEYIINLIKDKIKEEYGENILPEILMEKFIKLERKGSV
                     TVDVRDLIEG"
     gene            19400..20653
                     /locus_tag="ACOIAF_000037"
     CDS             19400..20653
                     /locus_tag="ACOIAF_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HGZ2667074.1"
                     /translation="MEVQGKYNKAKIFTDNVDSTTISQIINLCNQKEFKESSIRIMPD
                     CHAGKGCTIGTTMTISNKVVPNLVGVDIGCGMATIPLEKYINNINYTKLDKIIRDYIP
                     HGFKIHNKSREKLLKNLFNIDINNLRCEVNKNRAYMSLGTLGGGNHFIEVDKSEKGQM
                     YLTVHTGSRNLGKQIADYYQDKAIKYCIEKYKKSYDDAKEFLISTLKKDNKEHLINKY
                     LKDLSDRKLNKPHDDLCYLENDLMEDYLHDMSIAQKYAAANRMVIIADILFKYNSLDV
                     NYDHFIRDIKCDVIECIHNYIDMDSKILRKGAISANKDETVIIPVNMRDGIILGKGKG
                     NSEWNYSAPHGAGRILSRGKAKEKISLDEFEESMKEIFTTCVGQSTLDEAPQAYKPIE
                     DILNNIGDTIEIMEVLKPVYNFKSN"
     gene            20693..21094
                     /locus_tag="ACOIAF_000038"
     CDS             20693..21094
                     /locus_tag="ACOIAF_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667075.1"
                     /translation="MGMYTEFVCAIELKKDTPKEVINILNNMIEGEDRYDITPPHPFF
                     ECRRWRWLFIMDSYSFSGRSNTMFEYDDIAKTYYLTIRSNLKNYDDEINKFLNWIKPY
                     IQIHCEDDEFLGYSRYEECRNPELIYYNEIQ"
     gene            21121..21354
                     /locus_tag="ACOIAF_000039"
     CDS             21121..21354
                     /locus_tag="ACOIAF_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667076.1"
                     /translation="MKFEDNMIKSFREVRVGDIILAGNNKYLIISILNYFGYVNLDTG
                     DCSSGFRSIEELMKQFDKQTRFIQNDRFKLILD"
     gene            21397..21807
                     /locus_tag="ACOIAF_000040"
     CDS             21397..21807
                     /locus_tag="ACOIAF_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667077.1"
                     /translation="MCKFCESIIDNTKDILLTSRSRLLEDNTCEIIAEDNCNNCYDGC
                     YEYFKLNGYKIRGNTYVNVDYYKKIRKIIIAPCSESVHINYCPYCGKQVSKDIKDFNC
                     IPEHIVDVRYKNGDVYDYDMEKSVQEVMDNANNN"
     gene            21838..22011
                     /locus_tag="ACOIAF_000041"
     CDS             21838..22011
                     /locus_tag="ACOIAF_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667078.1"
                     /translation="MNLWSKIEEPVMRAVSGIVFNIWGNVCFSIYYIEENKQIELEIY
                     DRPYRYEGKKNSC"
     gene            22121..22264
                     /locus_tag="ACOIAF_000042"
     CDS             22121..22264
                     /locus_tag="ACOIAF_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667079.1"
                     /translation="MNKGIKNYITAVNYLLDNLEYMMEDDEFNNEKLQKRIDVIRKFE
                     REL"
     gene            22266..22571
                     /locus_tag="ACOIAF_000043"
     CDS             22266..22571
                     /locus_tag="ACOIAF_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667080.1"
                     /translation="MGKIKNGEIDTEDYRLFKNIQKEKDRVYSKYINEEYIMEEFINY
                     LRLEDKDYTTEILERYARYIDENLDNRCPYCVYNICSEHDNCFDGILENLKEKIKIK"
     gene            22597..22950
                     /locus_tag="ACOIAF_000044"
     CDS             22597..22950
                     /locus_tag="ACOIAF_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667081.1"
                     /translation="MKLINGDRCSGKTTTLIKYAYENNALILCYSCGNLNYIIEKAKE
                     LKLDIIIPRVFKTYIESNHKIEYMKTINGNIINDTSNIRLVIDEIDCCLESIIREGID
                     CVTGTIQIENLNNHI"
     gene            23030..23314
                     /locus_tag="ACOIAF_000045"
     CDS             23030..23314
                     /locus_tag="ACOIAF_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5052 family protein"
                     /protein_id="HGZ2667082.1"
                     /translation="MKKILLIGALGTMLIGMSGCTGIQSSMKDAESDVSGLNRVVNVY
                     SDNGEVLKTYTSKSMRVKDGDGGTITLDFDGKRVIICNAHVVIEENQSNK"
     gene            23326..23493
                     /locus_tag="ACOIAF_000046"
     CDS             23326..23493
                     /locus_tag="ACOIAF_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667083.1"
                     /translation="MFKVLKTILIIIWVVDILNFPQFQFLDTTYPINTLAWLLIWILI
                     PSSSIYIDKKE"
     gene            23510..23644
                     /locus_tag="ACOIAF_000047"
     CDS             23510..23644
                     /locus_tag="ACOIAF_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667084.1"
                     /translation="MILNIGAIFNLWCMLANYLWGSTSGMILNGFCLIALLLLDNNLD
                     "
     gene            23671..23823
                     /locus_tag="ACOIAF_000048"
     CDS             23671..23823
                     /locus_tag="ACOIAF_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667085.1"
                     /translation="MKITISIEEAMDIIKQHYENKGIEIKDLSFNLEKTGMVRNIKFK
                     GEIILK"
     gene            23871..24473
                     /locus_tag="ACOIAF_000049"
     CDS             23871..24473
                     /locus_tag="ACOIAF_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667086.1"
                     /translation="MILISSTNRSNARDFHVSDYYRTPITKIKEFLTEFSRYENILNP
                     NIKILDPCAGGDNTNPMSYPTAIQEFLNQDISIDTVDIRDDSRANIKQDYLKFQPKEK
                     YDVIITNPPFNLSLDIINKALNDVREGGFVIMLLRLNYLGGKVRQRLWGNNMPKYIFV
                     HNRRMSFTDDGKTDSIEYAHFVWQKGYNPKFSQLKVLISQ"
     gene            24488..24697
                     /locus_tag="ACOIAF_000050"
     CDS             24488..24697
                     /locus_tag="ACOIAF_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667087.1"
                     /translation="MKVKYVVFEWEITSKNDGQKHFINFRDLIKLYGVSSRECIRAKD
                     YYERDGLDLKDIKFLYPRDDGKYKL"
     gene            24752..24997
                     /locus_tag="ACOIAF_000051"
     CDS             24752..24997
                     /locus_tag="ACOIAF_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667088.1"
                     /translation="MDIKIDMCLLEMEINRRRCEYAARTGRYPNSLILWEGYYSLLNI
                     QYIEDKTRGYITKFNGMDVIKCNDFLKIELFEKNINN"
     gene            25026..28097
                     /locus_tag="ACOIAF_000052"
     CDS             25026..28097
                     /locus_tag="ACOIAF_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019595671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HGZ2667089.1"
                     /translation="MRYNNYHSHKIYSNIRSLDVITKPQQYIDRAIKLGQNTYFTTEH
                     GYQGNVYEAKTLCDEYKLKMIIGAEFYYVNDINEKDRGNYHLIVIAKNNDGYKQINKA
                     LSLANKNGYYYKPRIDEKILFEIFKPGDVVITTACVAGILKLENREELILKLKNYFKN
                     NFFLEVQSHPHKTQALHNKDVLELSNKYNIDIIHANDSHYIYPEESKYRTKFLKAKGI
                     NYPEEDGFILDYPNSEDIFKRYEKQNVLTKQEVERALKNTLIFDECEEITLINDDIKL
                     PSISKNPNKELKEILNKEWLEKRKNIPKNRWNEYLDAIRYEFDIIEKTHMEDYFIIDY
                     KIVQRAKQEYNGLLTKTGRGSAPSFIITNLLGLTEIDRLNAPVPLFPTRFMSVERILS
                     AKSLPDIDLNAEDAEPFIQATRDLLGEENCAWMISYKPLQDASAFRLWCKANDMKLSE
                     YDEVAKNLDKYTNDVFWKDVIKESKVFVGVVESVSFSPCSMLIYDRPIDEEVGLLKTK
                     DGVCCNIDGYYCDKYKYLKNDYLTVKVWSLIRKICELANISIPTIEELNILLDAKTYD
                     IYKDKLTCTINQVDSDYATNLASKYKISSVAETSAFVASIRPGFASLLDNFINRESYT
                     TNVKELDILLEDSYHYLMYQESIMKYLIWLGIEESESYDIINKIKKKRFKEKELKELH
                     IKLKNNWIKKVGTENNFEDTWQVVNDAASYSFNASHSLSYAYDSLYCAYLKSHYPLEY
                     YTVAFNLYNEDTERTRKLTDEIEYFSIKLENPKFRFSKSEYFFNRDTNSIYKGIESIK
                     FLNSDIGEYLYSLKDNKYDSFLELLIDLQGHINSKQLSILIKLDFFEEFGKSNKLLET
                     YDIYNSIYSKKQFKRDNLPCNINTMRKYSNKETEKIFKEVDTKELCSYLESQIENTDI
                     PTNEKIQAHFEFVGSCNIKDSNSNPRYCLVIDIDTKYSPKVTLYNISSGNIKIFKVSK
                     PIFEDNKIDVYDLVYLKSIKEKAKSKKVDGKWIRSNTETEWWIQEYWKIEN"
     gene            28266..28451
                     /locus_tag="ACOIAF_000053"
     CDS             28266..28451
                     /locus_tag="ACOIAF_000053"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667090.1"
                     /translation="MRIDELKEENAFLKQENEEYEEILNEWECVRKEIVATCKKKEIL
                     GEKPSELEMVLQKIEDI"
     gene            28451..28771
                     /locus_tag="ACOIAF_000054"
     CDS             28451..28771
                     /locus_tag="ACOIAF_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667091.1"
                     /translation="MSVNKIYEKKEYIIIKSKSGYIVYNTNKEFKEGHTHLKSYNAAK
                     TAIDLVIKRRLPKSYSIYFLTSLMRISNDVQYIKHISDIVYIRNNKGLKEKYINSKNI
                     KKNY"
     gene            28846..29967
                     /locus_tag="ACOIAF_000055"
     CDS             28846..29967
                     /locus_tag="ACOIAF_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfJ domain-containing protein"
                     /protein_id="HGZ2667092.1"
                     /translation="MKSYKEKQFLVFEFENGKNVKYNLATGEYVGKSGRIVKDVCTQL
                     RGYNLHEVINSFEDENYKCFLNFVDERVNRSKSSRAWGRRVERIRNIGSFLNEINNYS
                     EYEQLFSAGLKKIIYPIHCDIKSIPKGLISICRNYSITLSDRLIQGYSKNPNLYNNLL
                     SLELNSIRKTNLISILDSGYDRMIFDRLIDIYKYKPQSLINYIDNLMTYEALNGLEST
                     LNELYDYVVMMSVISDKYEKYPRHFLTTHKIASRNYNRLKTQFEEEVFNKRIDKSLEY
                     SYQDYKFIYPKNTNEIKDEAVQQNNCVSSYIQNVIDGKCHILFMRKKDNPSKSLVTIE
                     VRNGKIVQAKGKYNRDVNEREQEAIDKYNNRIERMKKIC"
     gene            29961..30392
                     /locus_tag="ACOIAF_000056"
     CDS             29961..30392
                     /locus_tag="ACOIAF_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667093.1"
                     /translation="MLIERTKIRLMKEIQGFEMLKIGDIFVITSVGNNGAIHFKTDYG
                     IGFMNYSEFEAYFEIVQQKKKYEWGAWSIRGDFSGAYLYRTNGKKVEVRKGNFKASAT
                     CHDSDEFELNKGVELCLARIEVKKANKQVNLVLDKINNKEI"
     gene            30402..30557
                     /locus_tag="ACOIAF_000057"
     CDS             30402..30557
                     /locus_tag="ACOIAF_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667094.1"
                     /translation="MICKFKERCENKNRDCDYCCYNPNAYLEDAFEWNGEGRKPTDEE
                     LDDALEE"
     gene            30602..30829
                     /locus_tag="ACOIAF_000058"
     CDS             30602..30829
                     /locus_tag="ACOIAF_000058"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667095.1"
                     /translation="MEGIINRPVFDLNTFFTGSAISYGYRGNSKHNALISSITPFQID
                     VAYISGNKVESDIISLHHVLDGQIEIELLKF"
     gene            30855..31415
                     /locus_tag="ACOIAF_000059"
     CDS             30855..31415
                     /locus_tag="ACOIAF_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667096.1"
                     /translation="MRWKNILVTGALIVGSTIHVFANTEDVVNNNINKELIADTARNI
                     NLMYKKEWEQYKNTKEYKLEIKRINLEKELEIKIEKLIPMTWKVSYYTSLNCENSRYG
                     AVTATGEKLRYGFVANNHLKFGTKILVDGNLKVVKDRGSNKYFGNSNAIDVFVPRIKG
                     ESDYKYYKRVNNMGRHYKEGYIIVEG"
     gene            31427..32092
                     /locus_tag="ACOIAF_000060"
     CDS             31427..32092
                     /locus_tag="ACOIAF_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667097.1"
                     /translation="MKVNGIKIPGIIDVSNVCVYGIKESIISSGYPMQQQIKDMREVN
                     ISEKDLQRACQLGKSKQGSGHDCFSKGIIVQMDLQWSEYMWRQWDRYHHNDYVSSQSK
                     MHRLTKFNIDDMCNKYVVKNTINFLNELIYDYNNFQELKLTASDEVTLRNGENIRATK
                     ETLWKIIISNCPSGLMLTARVTLNYLQIKSMINQRENHKMEEWRILCDFFKTLPKYKE
                     LMM"
     gene            32138..33031
                     /locus_tag="ACOIAF_000061"
     CDS             32138..33031
                     /locus_tag="ACOIAF_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667098.1"
                     /translation="MAEKRINYNQVFVSGEVLDILNIARMNQGTGQEAIRFTLKVETA
                     PNESVNVDYFTSMFKSDGSPNQMFLGIETIANEIKTRAEDGKGDIVRCVCSLDNNLYY
                     KDGEKKERFQISGTFCNREKYDDKGNPIDDKGEVVKSIKASQVWRVYTLIENIEEKED
                     DLGKYLEIKGLINKYGKQGCNMTFRIHNEDMIEGFKSLYKIGDVGLLEGTVKSMVVSK
                     SAGFGSRIKKSAFTFLEIEGGDEPLKDGDKIFSDKNYPFTDKNIEAMREKIKEKDEKE
                     KQRDVDKNGKTIEISDDDVPF"
     gene            33071..33628
                     /locus_tag="ACOIAF_000062"
     CDS             33071..33628
                     /locus_tag="ACOIAF_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HGZ2667099.1"
                     /translation="MEVKEKTMNDGFKKVVNEEIKTYISNLYSDRIDGLKESDSQIEI
                     TKALIAFHLDIESIPKDNTNPFFRSKYANLDTILKAVNPLLAKHDLILMQSAEDAGND
                     KVYIKTKLKHSSGEYIESNSAPFKPAKTNDIQARGALETYLRRYAVQSVLALSFEEDD
                     DGNSLTNKSKGKSEEENSTIKKSRV"
     gene            33689..34240
                     /locus_tag="ACOIAF_000063"
     CDS             33689..34240
                     /locus_tag="ACOIAF_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667100.1"
                     /translation="MNCKCRFCKKKLNTNDAYKVEHITSGGNKQNRYYCNEQEYRKEQ
                     QDIYFWKQCQLGIDYIMGYTVINNQKNKMLQEIIKGGYTREELYDCIIEKKDEVIELL
                     NYRKDIEEEYPKLCYVFTILKGSIRDITIKNKQMKYEKENERIYKEVEKSEEYYEIIA
                     PKKVIYNKRQSLFDKVKGVYQDE"
     gene            34281..35807
                     /locus_tag="ACOIAF_000064"
     CDS             34281..35807
                     /locus_tag="ACOIAF_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HGZ2667101.1"
                     /translation="MVLGTMLKSLTLFSEYKISESDFIIDKVKFFFSLGRIMSKTHNE
                     LDEISVAKFVSSNKLKSEYERYGGWNSLSSAMEYGKETNIAAYIDDLAKNNLLIALDK
                     RGFNVIKEMEHNGLKFIPFEIFQSMKCREVEEFYEGLISSCSVNSIKNNMKVENLLLT
                     KDDREKLKQKTEAGTPYNIIFEYTEKEIGLSNNEESKYIYSLPILSNRTNGIGKGGGI
                     NIIAGFSGIGKTTLLFFNYILAMIYRGEKIVIFANEQKSQYFKSMLVSFIAYNIFNYH
                     DLDRNKIDNGDFTDFEEDLMEKIEKFLRDRCFAENLKFIYMEEFEISEILRKSKELVT
                     HQGFTGIAVDTFKSEDSSDAHYTGKLIENSKLLDSFGNKYNVITMLSMQLLTAQENKS
                     SYLSAGDLSEAKAVKNVCGLLKLMRKVVNELELDSTNKKFFLKPYKLKYNKLKKTTER
                     EYIQFDAKDLQKEYRLLFLNKSRRGQDGDVILLRFYGKTGRLEEIGRCEKVYRGQLSY
                     "
     gene            35817..36878
                     /locus_tag="ACOIAF_000065"
     CDS             35817..36878
                     /locus_tag="ACOIAF_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HGZ2667102.1"
                     /translation="MEIKDLTNEQVINFMEYLGSDLSPKSNDRQLIFNTCLCHNGDSY
                     KLFYYTESKTFHCYSSCGHIGSLIDLLIHINKYEFKDAINEIKDFFGISNQPMLRKGF
                     RIKKKVEQIHDIKDIQIELLPTPKKPYVYKTFQQVPIEEWENENISFEVLKTYQVYYN
                     LYENQIVIPHFCWHDRARPVGIRVRNLDEDKAKSFGKYIPLWYDNRCYNHRLSLNLYG
                     LNVNKKSIKNSKKVIVFEGEKSVLQMATMYKNNPSVAICGSNFSREQKKILIDLGIEE
                     LIIAFDRQFKVKDDEEYVIWRDKIYKLVQDIKDVVNISVIWDKYNLLGYKDSPSDKGK
                     TIFEKLLKNRINIENFVKQ"
     gene            36983..37255
                     /locus_tag="ACOIAF_000066"
     CDS             36983..37255
                     /locus_tag="ACOIAF_000066"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667103.1"
                     /translation="MIKFLIKNVHMGDKLYCINNIGNYKKGEIYTVSDTSFFGLFHKT
                     KGENCLPIECYFIHLPKKEELESNPKSMTKEEIEDELGYKIKIVSK"
     gene            37276..38127
                     /locus_tag="ACOIAF_000067"
     CDS             37276..38127
                     /locus_tag="ACOIAF_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667104.1"
                     /translation="MSIFNKKYGRNVKITEEEPPSPPPKQPIYNRDNIQYDKEMERKL
                     LDIDRLKIELEKEKKIEEERKKVERVKENEGKEWAWVEGYKCTDKNMKCKEFQYELNK
                     AYSMDGDDINIWNWGFHLCLDLGDVFQYYAPNLSNRFFKVKALVKKADKYKYGKEYGE
                     VTLLSGSVLRGHKINKIAAKEIIFLEELTFDKLKLHIQNSFPEVNTKSKWYAICKLGE
                     QEYYHRMFMNQMKKIRFSDTFSQLLFDECRNYCEILEILNKAIAYSKENLSKDMIIYL
                     LMKDIRK"
     gene            38151..38594
                     /locus_tag="ACOIAF_000068"
     CDS             38151..38594
                     /locus_tag="ACOIAF_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667105.1"
                     /translation="MDKINKIDLIKLKEMNIYDNYQLKKIASKMILHLRYEIMDFCGC
                     GSPEDISFMIKGVLTAIQNKEKNCNLEYTERCYIFEIEFNNVCGIIGSKNDLIQEFIL
                     NALNSYGLLEHGSSIWGSWLSDYGKQILCAFEIVGDCILDVSYLD"
     gene            38636..39280
                     /locus_tag="ACOIAF_000069"
     CDS             38636..39280
                     /locus_tag="ACOIAF_000069"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HGZ2667106.1"
                     /translation="MSDLHGMYDKFISMLEQINFNSNDHLYILGDVLDRGDKSLEIID
                     YIRKYKNITLLKGNHELMFQESYTDVNSSFLWFYNGGKDTFYDLGMKSYEYKENFYKY
                     IKNLPYLEIIDNFILVHAGLYLPNNYENLSIEQIIELQEEDICVWDRTILNTNKHIKG
                     YTIIIGHTPTQNIKDYEKAEIYKQEGIINIDCGACFDNGKLACLRLDDMKEFYI"
     gene            39291..39818
                     /gene="yyaC"
                     /locus_tag="ACOIAF_000070"
     CDS             39291..39818
                     /gene="yyaC"
                     /locus_tag="ACOIAF_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protease YyaC"
                     /protein_id="HGZ2667107.1"
                     /translation="MDILKISYNNLNAISKISEVLRDIVNEDTLIVCIGSDRVLADSL
                     APMIGSILEKSTIRNKIFGVLGDSIHALNLEQKIQAIKNNYPNSNIIAIDACISKISD
                     KGTIIISNKPVKPGLGVDKKLLEVGDYSIVGIIGRNKYDIYDTSDPELILDLADVISK
                     SLISILLEKEERMIV"
     gene            39815..40069
                     /locus_tag="ACOIAF_000071"
     CDS             39815..40069
                     /locus_tag="ACOIAF_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667108.1"
                     /translation="MIQEVRQEILLKNGLILYTGDLVEIKYKSDEDVIEHTCKGKIKE
                     AKELFIKLDTSKKYKTSERMIYSWELKEIKKVDDEDEKDN"
     gene            40053..40310
                     /locus_tag="ACOIAF_000072"
     CDS             40053..40310
                     /locus_tag="ACOIAF_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667109.1"
                     /translation="MKKIINSKYRALYLFIFFLIFNLIYSLYFGRNTEIGFYSSTSCI
                     EEYILDIITSIGLFFSMMLAGFDVTENFINSLRDYCEEDAK"
     gene            40312..40530
                     /locus_tag="ACOIAF_000073"
     CDS             40312..40530
                     /locus_tag="ACOIAF_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667110.1"
                     /translation="MNKLELKDYISKVELNDMPLTLDNKYMLNEISISEDSKIFAVKD
                     TSGITSYYTEIEFNKLFRGINSILENII"
     gene            40605..41444
                     /locus_tag="ACOIAF_000074"
     CDS             40605..41444
                     /locus_tag="ACOIAF_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HGZ2667111.1"
                     /translation="MEKELYSFSKLNAFNTCPFQYYLTYVKNLNREQSCYGYYGNELH
                     SLLEQLQQRKITNQEAIQKYNEVIEYANLMDYNFPTPNSRINYLECILHYIENFVPIE
                     CDRYYIEEYFEFDINGITMRGYIDLYYIIGNKIYVIDYKSSSKFSKKDLPKKSMQLIL
                     YAMYLKEKYPDKIIEYVAFDMCKYMNNEKGVLIERNKIDSINDYERAIVKIKYTKELE
                     KQLIDFVVDTVTQIKQLDSNDESVWCKSDDKSNQFFCKTLCSHYNKGCKHKENKSFRK
                     SKS"
     gene            41482..41802
                     /locus_tag="ACOIAF_000075"
     CDS             41482..41802
                     /locus_tag="ACOIAF_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667112.1"
                     /translation="MNIPKQVKVGGLFYKIEETEEPILVNNQLCYGLADYGTETIKLS
                     NNLQSERVKEATFLHELFHCIFRDRCIESEDEEYLVDVLSKGLHQVIIDNPNIFELKV
                     SIDG"
     gene            41795..42376
                     /locus_tag="ACOIAF_000076"
     CDS             41795..42376
                     /locus_tag="ACOIAF_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HGZ2667113.1"
                     /translation="MDKLKNIFVFLGYSGSGKDSIVTEISKQFNIPILISHTTRPPRN
                     YLEIINKTYYFVDNKFFKKEKNNFIEMRKYIVHDGSTWLYGIHKSELENKKYALTIVD
                     ASGYKALEKYFTGTKTKLVPFFINADEDTIRKRLIDRGDNLKEIERRLVDDKIKFKEF
                     LNSENYIVIANNLSITNAVEQVKIHMKEGIQWS"
     gene            42367..42861
                     /locus_tag="ACOIAF_000077"
     CDS             42367..42861
                     /locus_tag="ACOIAF_000077"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HGZ2667114.1"
                     /translation="MVLALDVSMSSTGWAVINRNKNILKYGKIVTKKDKFKSEDERMC
                     YICNAIQELIITHNIQIVLIEDQFTSRNSKTILSLRKLLGAIMRTVKLNNIEIEYMYP
                     VSIRKYLLNNGKAKKEEVAAYVRENIIDIGEYIDRTCKAKTSDIYDAIALGIAYLNKL
                     DTNR"
     gene            42885..43388
                     /locus_tag="ACOIAF_000078"
     CDS             42885..43388
                     /locus_tag="ACOIAF_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HGZ2667115.1"
                     /translation="MSINVKIKKLNQNAIVPSYAKEGDSGIDLYTLKDTVIPAKSAVA
                     ISTGISLEIPYGYEMQVRPRSGISLKGMFFTRYVTIYDKVQPKKQIRKIFVAHPTVRL
                     GTVDSGYRGEIKIITYNEEDFDILIPKHTKLAQGVFQQVPRAILKVVDELTSSERGSN
                     GFGSTGI"
     gene            43553..43726
                     /locus_tag="ACOIAF_000079"
     CDS             43553..43726
                     /locus_tag="ACOIAF_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667116.1"
                     /translation="MYRYDDYVNLINDLELPKEIRNKCVRVIMTLENMQDIMVVHKFK
                     TYVVNEKYIEKVA"
     gene            43846..44316
                     /locus_tag="ACOIAF_000080"
     CDS             43846..44316
                     /locus_tag="ACOIAF_000080"
                     /inference="COORDINATES: protein motif:HMM:NF019976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HGZ2667117.1"
                     /translation="MFKEIFEKVFMSEEVSKTVKEVNNIIKDTKIQVKGGFGELRLDF
                     ILSRLGKDYIIIKDIIVPGSNKTTQIDSIVISMYGIFVIECKNFSGYIYGNDKDKVWT
                     QIVGKTKNTFYNPVRQNYAHIKAIENIIGNKYNIYSIIVFSDKATLKKCNDKWK"
     gene            44300..44596
                     /locus_tag="ACOIAF_000081"
     CDS             44300..44596
                     /locus_tag="ACOIAF_000081"
                     /inference="COORDINATES: protein motif:HMM:NF013555.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HGZ2667118.1"
                     /translation="MISGNNIIVINESEILSTISKCKDVTIDKEEIKIIRDKIFDCMK
                     TTNQNTREHVRNLKNITEEDKCPRCQIGHLIKRKGQYGEFWGCSQYPKCKYTRK"
     gene            44885..45997
                     /locus_tag="ACOIAF_000082"
     CDS             44885..45997
                     /locus_tag="ACOIAF_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGZ2667119.1"
                     /translation="MNAFIRKRNKNYVVYLEFRDDESGKRKQKNMGAFDKKRDANKRL
                     AEVKDSIYKDSFLVPNEITLAEFLLDFLEKYKDNISASTYKSYIAICKNHINPSIGKY
                     RLQELRNIHIQNYIDDLSGNLNPQTIKVHINVLRLAIKRAYRIKLIKENIIDGIESPR
                     IKKFKNEIYDKEQMLKLLEVAKGTNLELPISLAIGLGLRLSEVLGLTWDNIDFDENTI
                     TVNKITSRLDGSVILKEPKTESSVRKIFAPIELMNLLKNYRLEQNKKLLRSIVRNEYN
                     LLFFDRKGNPIAEDVMSKKFRKFLENNDLPHIRFHDLRHSHVTLLINSKVPIKVISER
                     VGHSNINTTLNVYSHVLKEMDKEASDRISENLFKAN"
     gene            46216..46362
                     /locus_tag="ACOIAF_000083"
     CDS             46216..46362
                     /locus_tag="ACOIAF_000083"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667120.1"
                     /translation="MIERRNPNNTIPDIILFCILFMFKIDKTTFESNMTILAAKKVVP
                     FLFV"
     gene            complement(46438..46923)
                     /locus_tag="ACOIAF_000084"
     CDS             complement(46438..46923)
                     /locus_tag="ACOIAF_000084"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667121.1"
                     /translation="MNKKCKKIIATSLIGTTLFTTLLPSVSFADSNSHQTGIESYILD
                     KQKLKDIDEIAAYFGFTEQEKADYINAIKEVKLEAESTPTTRGKLSWAVKILRIAWTK
                     IPTKVKVAIGGYAAYGRILSVLNTFTGTVEDGLYKGCKSLGMSNDVAWWVTKALLAFV
                     L"
     gene            47277..47411
                     /locus_tag="ACOIAF_000085"
     CDS             47277..47411
                     /locus_tag="ACOIAF_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667122.1"
                     /translation="MENLMMSIIAGVIASYIYDKLKSHSERPTKSGWELNIKFHKNKH
                     "
     gene            complement(47518..47739)
                     /locus_tag="ACOIAF_000086"
     CDS             complement(47518..47739)
                     /locus_tag="ACOIAF_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667123.1"
                     /translation="MKLPELEINISISIPIFLVISYLAIVSIVMLGISALNNFTNITS
                     VPINALGSTVGFVIFQLLVSFFENRKITD"
     gene            complement(48007..49281)
                     /locus_tag="ACOIAF_000087"
     CDS             complement(48007..49281)
                     /locus_tag="ACOIAF_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HGZ2667124.1"
                     /translation="MKFKRKALTLAIISSIVMSSTLLVSAKELKFRPPAHRIQGANKY
                     ETAGLIADRRKYTQAIIINTDKSLADGLSASGLAGASNSPILLTKQNSIPNSTLKRLD
                     KFKKIYLIGGVNSISKNVENILKNKKIKVIRIEGKDRIDTSYNVAKEISNLKKVDEVY
                     LTNAYQGEADSISISPVAAKYKNPVVLTNGKNIPFKTDGVKTYAIGGTASINDSLVNS
                     TKATRIGGVDRLDTNEKIIKHFYKEIIDNYKYLNQICIVSSDNLIDALLSSTIHKEYP
                     VFLVNETNDKSLATNAYYPIIIGDIKNEILDQCLTPDYMLIGSIERSDISKALNAIYK
                     AKGSNAHDYCYAYNYDSSGYKKAENIDFPYYEFQIISIKNVKQDESSTDGSDTYTDEK
                     EDSIKTVGTLIVNSETLEVYEYSFDTQKLTKI"
     gene            complement(49436..49756)
                     /locus_tag="ACOIAF_000088"
     CDS             complement(49436..49756)
                     /locus_tag="ACOIAF_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HGZ2667125.1"
                     /translation="MQKIPQAELKVMKFIWNKNDIVTSKEVMEAMESEYNWKATTTLT
                     LLSRLTIKRFLDSERIKRITHYTILITKEEYKIFETKRFLEEVHSNSIESLISSLEDC
                     YKNK"
     gene            complement(49894..50244)
                     /locus_tag="ACOIAF_000089"
     CDS             complement(49894..50244)
                     /locus_tag="ACOIAF_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGZ2667126.1"
                     /translation="MLEDEIYNVIGKRIRNYRRKAGYNQNTLAKKAGLFPAYIGQIER
                     GESKASLRSIFKIANALEIPLEVLFENIIQNENDVETLSSEAYELIDSLTVKEQKAII
                     RLIKEIIEYRKFEE"
     gene            complement(50526..51464)
                     /locus_tag="ACOIAF_000090"
     CDS             complement(50526..51464)
                     /locus_tag="ACOIAF_000090"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGZ2667127.1"
                     /translation="MATFEKRSKRIKIEGNINKNNEKLIKGFLQAKKIAGLSEKTIYV
                     YKCDLYTWAKYLHDEMEDLLIEDCTEDDIMEYISFRMEDNHVNRIKVIFSGISSLYDH
                     LRRKRIVTENPVSLIERPKKGLPVVEKHFLNIEQAKELQDKLSKRDDLQLEVYINFAL
                     STAGRVTATSNLRWDNIDFDNRVCENIKEKGGKIVDFYFSEKVKDLLLKLKEVRKKED
                     IVNDYVFLVKYDKEYHKATSNVLRSWAKKAGKLIDIDNLAPHSLRRSFATIAKANNLP
                     LEDISTILNHESTDTTKIYIKEDKGKISKSKDMIGL"
     gene            complement(51499..51810)
                     /locus_tag="ACOIAF_000091"
     CDS             complement(51499..51810)
                     /locus_tag="ACOIAF_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667128.1"
                     /translation="MLSKEMIELLSQYGYTAILLTVVMLWFGRYLEKNRQLEQDDRKK
                     ERAYFSREIKEQRTLFGNTIDKFDDKLDKFAEALNTNNSRLERVETDITKIKDKLETR
                     D"
     gene            complement(51811..52062)
                     /locus_tag="ACOIAF_000092"
     CDS             complement(51811..52062)
                     /locus_tag="ACOIAF_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HGZ2667129.1"
                     /translation="MDIMQFIPENLIMLIGGLYILGTFIKESNIKNKYIPFILLVIAM
                     ISSCLFMKELSIEAVFEGILCWGASIGINQIQVQNRKEK"
     gene            complement(52074..52691)
                     /locus_tag="ACOIAF_000093"
     CDS             complement(52074..52691)
                     /locus_tag="ACOIAF_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HGZ2667130.1"
                     /translation="MNILDKSTATLEQVFSYLDTLKNNSNPPHFLCRAIVPIIYKEAE
                     KKGVNPVIAIAQAFVETGYFNFGRVLNPSYCNVCGLKGNKGGGDLDPTAHTRFNCWED
                     GVSAFIDHLALYAGAKGYPKYSELVGKVEYKVNGTTLDPRHFPYLHGEAKTVESLSGK
                     WCPDMNYGQNIINICNKISSMKVENNDVKLEQIKTKVKELNEILG"
     gene            complement(52703..53518)
                     /locus_tag="ACOIAF_000094"
     CDS             complement(52703..53518)
                     /locus_tag="ACOIAF_000094"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HGZ2667131.1"
                     /translation="MNIKTLTIHAGHNPDGKVGSGAVGNIKESTEARNVLKELIPLSQ
                     KECKVYDCTCNNGTSQSDILNKIIDKCNSYNTDLNVSIHFNSGGGRGVEVLVYNLNDK
                     ETVEIASRICKKITETYHAKGDKDFKNRGVKEKKTLAFLRRTKAKSILVECCFVDTSD
                     TKKYNAKDMAIDIYEGIFNKSVAGKPQDNKVKYAIVYEGEVDKVIAQLMAMNYKSNEV
                     SICELKNYVPGHCENLYVIGRASSKIKTSERFTKLQGDDRWATLHKVLDFIGK"
     gene            complement(53644..53826)
                     /locus_tag="ACOIAF_000095"
     CDS             complement(53644..53826)
                     /locus_tag="ACOIAF_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667132.1"
                     /translation="MNINNVVVRILAERILNGGLNPLKNREFQLDDVTNIGYRKAVED
                     YIIEHSGVVEEAEPIV"
     gene            complement(53826..54119)
                     /locus_tag="ACOIAF_000096"
     CDS             complement(53826..54119)
                     /locus_tag="ACOIAF_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667133.1"
                     /translation="MNRANRIICDQTGKILLQTGEATGDILEHDEITELHFVDIPYGS
                     VDYTKSRIVSINIETKEPILEEIPVFVSEEEKRIQELENQLLIAENEKVGGIL"
     gene            complement(54133..55857)
                     /locus_tag="ACOIAF_000097"
     CDS             complement(54133..55857)
                     /locus_tag="ACOIAF_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine rich domain-containing protein"
                     /protein_id="HGZ2667134.1"
                     /translation="MQTEWNFNYANYVQNVSLPPGRYKLECWGACGGTLGASDWTECA
                     KGGYSKGEVILKKRTNLCICVGQSGYEKLDTGSTLTRTGFNGGGRTNAHGINGNYYSK
                     YGGGATDIRAYYTRATWDSAEGLLSRILVAGGGGGTKNNYAAAHSIGHGGGYVGENGI
                     GRDRDFCGGGSQYQGGTSYDTEEYHGSLGKGGYGGIGVGGGGGWYGGAGSYSNECGGG
                     GSGYALTKDSYKPPGYIPTSEYYLENIVMTTGGNTTKADGYAKITLLQALPFLTVSSY
                     NSTTATFKADHTDPTLLTKIEYFIDDVLKETITTDLTLEKTINYTLEDNALHTLKIVV
                     TDSANATAEKVVSISKGIAPLPAGSTTDEVTNKWIEIKDTFKSGKTSIINTLALKNIE
                     ASLNNTLVELSEKIKTSFDSSDASAQDLMNQLTQANNTISQLNSKYKYASGTIDVVKN
                     SSLMANLYGESFGRQPGTWLKIDNLGFIPNIFVAECQYVTSNNYFFKHIVIATCNINW
                     FWDKKDFSARIVFTKEKNSNQDFSGSGIIYSNNECDVYINNKGINVPASSPNVSSYLH
                     SWHAIKFI"
     gene            complement(55875..56204)
                     /locus_tag="ACOIAF_000098"
     CDS             complement(55875..56204)
                     /locus_tag="ACOIAF_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667135.1"
                     /translation="MLNEEVVKKLKNVPSDTNNAIETAKTEINNKIGDTTQLTTTDKT
                     NIVSALNEVKTSVDSIETTAEKTSYNNATSNLTATNVQGAIDEVVRKIEKFNEVNISI
                     QNDMLPI"
     gene            complement(56283..56609)
                     /locus_tag="ACOIAF_000099"
     CDS             complement(56283..56609)
                     /locus_tag="ACOIAF_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1375 family protein"
                     /protein_id="HGZ2667136.1"
                     /translation="MNVNINEEKIEKLKQKKKISNLNISQKTQDNDITDLMLATAEIC
                     EMVLSSQQTSTMSLRNIKLNEGGSSMAAIYVGLIERGLKTIDQVPVKYREEVRKMCEA
                     LEISLS"
     gene            complement(56623..61506)
                     /locus_tag="ACOIAF_000100"
     CDS             complement(56623..61506)
                     /locus_tag="ACOIAF_000100"
                     /inference="COORDINATES: protein motif:HMM:NF012632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RCC1 domain-containing protein"
                     /protein_id="HGZ2667137.1"
                     /translation="MKENKLIQRGRYFDTIYEIEDFEPEFDKYDFVKMFTSKYIDCTY
                     GLKSDETLWVTGNNQVVGKYSPRNNNISRRVDTFTKIDGVSNIKDIAIGSEHTVILKK
                     DGTLLGVGQNSNGALGLGGDITVATTFTKLPIEDVVKVVVGSGLTIALKSNGELYFTG
                     YNGSQQFDNLENTNTFIKLSITDVKDIDCGDGFTFILKTNGELYSCGSNSYGQLGLGD
                     NTNRNTFTKVNIDNVKEISCGRYNSFIIKNDDSLYGCGYNTEGQLGLGDITNRNVFTN
                     MALNVKKVVYGINSTIILKNDDSLYGCGRNTYGQLGLRNVPNPKTFIKIGINNIKDIY
                     CGNNHTIMIRNDNKLLFCGTNRESQIGNPNNINYPVDVFTEYKSAEVLGYNKSVTMNN
                     KNYLIKENGDYICYGNIKKIVSVEGSTYALLENGELWVSGNNTDGQLGLNHKVTQNYF
                     TRVPIDNIRDVVCGAYYAFIIKNDDTVWVTGLNNSGQLGTGNTSQLQKFTKISIDNVK
                     KIACGRSHVLILKNDNSIWGAGAGYYLGIGTTSSTGITSFTKVPIDDVRDIACGYECS
                     IILKNDNSIWGAGANHSGQLGKTSNNSVEKYFIELSINKAVKVKEIYCGIYHTLILKT
                     NGELYGCGSNTQGQLGRRISTDKLEQFTLIETDTTHICNNHCESYNTLIIDSIGRLKA
                     TGQNNYNQLGLENNKTTPIESFTPTNIVIGQGNNRYIAGGSDCTFLVKTDGKVYGIGN
                     RLNGRFGTGSDSVAASTFTRIGFEFNSTEIDYNNDFRIATTNVNDYMSVFLKKSNGEL
                     YGVGNNTYGQLGIGNNSTCHYFYPINIKNIKDVICGLNYTLILKKDGELLVTGNTSSQ
                     KALDTKTLLTSEPYRFNQVSTISNVEKVVCGSLIVFALLGDGRLYSTGTNTYGQLGLG
                     DIANRDSFARVNINNVKEIACGISHTLILTSNGELYATGRNDEGQLGLGDNTNRNTFT
                     KVNINNVRKVICGAYYTLVLTNDGELYATGRNKEGQLGLGDIVNRDTFTKVNIDNVKE
                     ISCGLDHTLVLKNNGELYGCGSNGQGQLGLGDNTGRNTFTKINIDNVKGINAGGRSSY
                     IYKNDGSVFSTGQNTYGLLGLGDVTNRNIFTKVNIKIFDFEKITDCIVNNNFNLVNNK
                     FLIPTKDTKYQHYVTSSDDFNYNEILGSQYPVSPYANEVLELPFNNIKEFWMSKTHSL
                     IVNTDGELYGCGKNTYGQLLNPLTTTSLLEFTKLNFDNIKQASCGNGFSYFVRNDGAL
                     FSCGLNSNGQLGLGHNNDVSEPQLITTISNVKKVMCDNNFTLALLNNNELYAQGYNRF
                     GNFGLGEEKKDTIIPLFTKIATNVEDVEIGTNYILLRKTDNTVYISGRIRDCYNIEEN
                     NIYVFNEIKTPSSIYKENLVWVNPVNDETVSLIYKVNSVNSTIEIIEKTISGIKLKIN
                     DDGNEIIKIEMYINNELITTMTEFTNKYSQFTIPLDKLVLGRNNIVFKGYDNFKGNIY
                     TSLTINKENNSIYVSENSSLLINGKRYTVQSVSESEGKVTVNLDRGLEGNVNVGDIIY
                     QLINRLNVQIKTNNTGMHKDAKLLEMKKVDTGYQEIYEFKENGIKEVEPKIIVNGNED
                     TAIKRPSMIFSIDETTL"
     gene            complement(61523..63598)
                     /locus_tag="ACOIAF_000101"
     CDS             complement(61523..63598)
                     /locus_tag="ACOIAF_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HGZ2667138.1"
                     /translation="MKNYEIKNHTIEVDFSNYRINKRLLDYFIYNENDVKTAYIEAIL
                     KNKDEIIDLSEYDRVLVSITKSDGQKVNGECEVVDAENGVVEIELSRQALASVGINTF
                     QLSLVKEGTLLNTTNLYYRVEEGMINDDDITSTDEYGVLLVIIAQAEEIIKNNKELTK
                     RVEQLEITILGNEEVRDKAEQIRIYNEDIRNIQEEEREFNELTRQNQEANREESIQNM
                     QIQVDDKLTDCQLQLDEMIDAKSEEIDNIVDDKMLDVQAQTDKKFQDLDTRANNIFDL
                     YDKTFEDKLTDNQEQIDYKLDEVNQAISNVEDCIDKSTTKLDTKIKEIDDKIVEVNTA
                     KTDMTTTVSNKITEFENRFSELESLDARGELIQARETIDGNVKDTLKDRLTYDFEKVN
                     EKIAEMTSAATNVAFSKSYVESDWVADGEYFKLIVNHNLVTENIFVAILDEATKKSMT
                     NSYTIVDSNTIEIFNESNINVRVTVVNGNTNKEVIQATINDNITTLDSTYSSVKIDAK
                     IDESLIKINENKSNIATNLEKINLIQNKVGSSELSTISKNISDAVNELDASVKILEAG
                     GNLGQQLDSLKAKYDALSNKVLDIAVYLELESGLLPDEVGHWFDGLSDDKAILSIDGD
                     LKLDTENRKIQGATGSVTFNRITIPFECKKLRYIHELNDNYIETVSDKNISIGTTIID
                     LDKYSYEIK"
     gene            complement(63610..63765)
                     /locus_tag="ACOIAF_000102"
     CDS             complement(63610..63765)
                     /locus_tag="ACOIAF_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667139.1"
                     /translation="MEVNLQKAYTVAFEEIKSLYNELILYKALNMQQQEEIENLKKEL
                     EEQNKEQ"
     gene            complement(63767..65695)
                     /locus_tag="ACOIAF_000103"
     CDS             complement(63767..65695)
                     /locus_tag="ACOIAF_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HGZ2667140.1"
                     /translation="MILDNSPADSWIRITGVIVTYNNTLYQVVDTETNKKYIYWDADN
                     PGTLKVSNVRLPEGNTQFLVVVNDNGKHTEVPINSSIFDISFDGNSRKNTEEQIWGLY
                     ETDKKHNEKFVVIEKDIDGIHQTVLEVQEDASHIKENMSLIDQRAENVNILVKEVTKN
                     FGQSQENITLRENINKAIIKLNADLGTFSSNMSNYFNDNEITDEEKEKIDIELNLLDT
                     DKASLYAELQKLIDKTTGVDLVAINTSKTALDTASTNLNSIINSVISDSIITPSDRIL
                     AINANAQYNLKINELKNTVDRIYITGMGGSISEEFSQINATAKEIKLEVARVDGVTKT
                     NAAEIKLTKDDITMMVTRNGSGSIVGIKPDKIEFGFNDISNYVEISRSGLTVNQGAIA
                     CDILTTPSGHEPIIRLFGSSRSGFAIDARRSDGSSQASAIRLKYDSNNYFWVGYDTAE
                     IYVDGEEHFIVERDDTFVRCGGATFTFTNGDSLGIGYSFYPERSSTDLGCDDYKWRYL
                     YSRSTLSESDKKFKENIVYIKDIKNRTRSSITPTPFLDFIKDEFKPATFDYIVSAEKD
                     RTIADSQIGFIANDFKDSYVGKTFLYDYGEENGLMFSPSGYTTVVATALQEEIQKREE
                     LEMIVSELNEKINNLGGY"
     gene            complement(65697..67412)
                     /locus_tag="ACOIAF_000104"
     CDS             complement(65697..67412)
                     /locus_tag="ACOIAF_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667141.1"
                     /translation="MHFNVNLKQIKSDYTLTIHKMNKSFLGQIPINFLNSIKRELGGV
                     DEVQLTIPKYITERFQFSKVINPLFNEIKEERLICLNDKEYFVIKNVVTTDDKLKSVT
                     AKSKEVKLGKIDVNIEDYGLQMFTKDEETSIISLNDYLKQETGWKLGHVDDSIAYETD
                     SEGNKREKVRWQESINSNWLDYFNNELKEQFECIADFDTYNNLVNLYHIDSFGDNIQL
                     YLSHDNYIKSLERTTNSDDIVTRLKLEGSEDMDIIGATVTGYDYIENYSYFLDNKEMS
                     EELSRAIKKYQEMNEIREPIWRELIDTKLKKQRERDDKSSEWLHVIETIKAKKDIKKT
                     YDNPEHKDEVNSAKLAVEISELEDKKVILDVQIKHLEEEIAKLDESIKDINILCKRET
                     STDEDGYLIFNEVLLDELNEFLYYDTYTNDAFLKVEDLIAEGKRQLSLKCIPTKEWTL
                     DVINFLDRIIDINFRQHWKGDLSMGDIIVLHSKESKEEELVYFTSFTQNLKNGKLDTL
                     ELTLSNKKIKEDDKRTIADYLTKAEHATRTLNSKRHLFIQQQKKRINLPDEYIPKKNI
                     QKELM"
     gene            complement(67765..67920)
                     /locus_tag="ACOIAF_000105"
     CDS             complement(67765..67920)
                     /locus_tag="ACOIAF_000105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667142.1"
                     /translation="MINNIEDCKLLIEAEIRMKDIDEKELISQRDILKDLNINEKDLE
                     DIEVNFE"
     gene            complement(69070..75144)
                     /locus_tag="ACOIAF_000106"
     CDS             complement(69070..75144)
                     /locus_tag="ACOIAF_000106"
                     /inference="COORDINATES: protein motif:HMM:TIGR01760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGZ2667143.1"
                     /translation="MEDKRKRGGSVLHEYGVSLGDTVGMITATNEAIQNPEKVGNGLK
                     SVAINLAGMKVSADDGTLSLNKTAKGLKEIAGITIYKNKDTGELKNMVQLLDELKGKW
                     GELREDEQKGLAEAIAGKQQAAIFQSLMGNYETFKEIRSEFSQGLQFGSAEAENKQYL
                     DSLAGRLNELKTIWADIFQTVFNSNTTYGFLDGLISVSNGINSVIKTLERLKLTTPAL
                     IGSFMTLKTLFSGMGMTNGTRNVARLINPLNMIGTPIKATTTILQSFRHAVMAANVAG
                     ANGANVITRAGTGLGALRQSLSRTSIGIGTLNIATSLLNGAMIGLATFAITKGIQWID
                     DTIHKYEKLAEKQKEVIGTTTSEISSLQQKKKSLEGISEEYNNLASKTEKTADETERF
                     SELKQQIADIAPELVLGVDENNDPILNMSGNLDEVINKLRKAIEEKNKLLKSQEDELA
                     ITTSKTAPDTKKDLDKEKKKLDKVLEDEHNLFKNALSKSNFKEQTEALKKAYNQQETD
                     FAEHHQKLLTEYEKYNEEEEVMQKRAINRLTNSDISKTYAKQSEQKKSKMNELVANMN
                     WHDYSVGQQNSIISAFDKISESIPVEKLDQWNKKITDANINYQASGDMNQYTKELQGL
                     ANELQKLSGGSISADTWLIGLQQGFSGLDLASMKLHTFLKSYGSSLAELENKDSFAIK
                     LKNQFDALNDFSQDLEVNGKVNIKLLEDIQSGEAGQDLPQQLKDIINGFTFGEDSQIE
                     AQAQLALQVTTTLQNEGQLSNDVVKQLNDLFKGKLQLTDDILKNGLKISDKLPALPGN
                     MVQALNEQFANAFTGDNAPEITPSVNGEKAKKELKELEKFKKDTFKSDKIVTEINNKG
                     LDFAKKDLSVLKEQLDRFKNEDTKLSFTTDCADAFKDVNSLEEARKKISAPMSLKYDI
                     DVDKKGLTEAKKVYDILPDNLKKKIETEVIGIDKLDTLQNLIDKAPNKKETIFEIINK
                     NPDKLNEIDEILNNVPNDKKEAVIDFIVNNLDQLEKAKNVQEFINNLPPEIKKQLGIE
                     VEGGENAEKTVNTVQNADGTKADLLVNAEVNGESQVVECIETLDKNGNKTYTPNLNPQ
                     VNNSEKVVQCMRILDEYGNESYIPIVSPQVTNEEGLNSTKQKMDELDNKTVTPTVQIK
                     TEGETEVEQATNFIENTNPTKVIELSINNSGKDEIDNTKQKLDELNNEKATPTIEATG
                     AEQASEQLNQLSQKAQEIGQGNYHINVSTSTEQGAKNISGLIARVKQFLALKVSTLVF
                     RTETAQAAKNVSGLLAKVQSYVSRYGGKTIKTTFSALTATAAKNISGLIRKIESFKSN
                     YAGKTFTTTFVTNKVTNSSGGGGGGNSSDKTNKTERTSVTNGTPIPANLSQPRTSEPA
                     PISDETPVTKPSLFSRAVSRATSAFKVIKPKVTINNDTIDSSVKYSIELFKELENAIS
                     AVTNEIALLDKKMEHATGDEKIKYLQEQNKLFAQQKDLIKQQQDGYKAESNRLKNRLK
                     EKKMGFKFDDTGNLTNYEEKLISIEKELDKLNSKSGDKENKKDESRKKELEETKKVLE
                     EYIKVTFTELPKCEQEWEEYNNKIKEHNEELRKAQEEMWKLNKNSTWTSMYKDVEQVN
                     NEIAMIDVLLKNATGDEQIELLEKKKELMAKKAKELSETTAYLQETQKELQQKLQGYG
                     FTFRDNGDITNFIQHKNQLKKNMTDEEFKRMEDAAEGYLDLLINKIPDANKEIAESKK
                     EIEALNKEIQDAYKDQLKEAQSLQEKIRDMYKKELEERLKEIDKETKAKIDSLKKQQD
                     AYNDSRKEAKYKDDYEEQQDVISDLEKQIAIAERDSSLSGQKKLKDLQKQLKEEQKKL
                     QDLVQDHVDDQVNDMYDKESDRLQEEADKLKEELEKKYSDENLVDLINEAISSGKFVG
                     LDGEVEKLQDAIIEYINKYEDGMLAMGSVTKQEWLDTLKEGKETLEDINDILDELDLS
                     KFAMPNYNPPSNSRSRSAFPTSSVNFNSPFFVVQGNVTKDTMKDLEKFGRDLENRVYR
                     KIVENIKS"
     gene            complement(75748..77226)
                     /locus_tag="ACOIAF_000107"
     CDS             complement(75748..77226)
                     /locus_tag="ACOIAF_000107"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667144.1"
                     /translation="MVEEFKIKTSIELDDKDAREKIEKLKNSGQDKPIKLNLKIGELD
                     KLSQQAEQLKGIFNKAFKIDNTALSNLKQIESTMKEINKLSRRSQESLLGGGSGNTGN
                     INNEQQDLIRQYKEISNVVSRLQNQIRTGLGDSSIERTTEQIERLKRQLDSVYAQMNS
                     SSQARMDMFDASRATRDLADMNRNLSRIEDMATQASARLNGLGDNIDTDKVNRLRNEL
                     QELKQTASRNIDLNLDVGNIVNRIQEINQEVRNLERVDSLRSNFSRIEDSIRQAFGNE
                     RVEAFRRSLEQLQGSAGNLNGDFNNIFDRLTREMRDARQEAERLNNTVSRGGGFFNDF
                     YSSVASYSIGDMLGDALTDGIRSMKDVILELDSSMTDLKKVAEPTDIDSTSELDRIRE
                     KAIQTAKDVGMSASQVQESIASSLQAGIGGMQESIAAARQSMILANVGDMDVEGASSA
                     VNTIINGFRIEPLKEMQVQVGNTTKKTTELTAAMDMLNCLAC"
     gene            complement(77306..77755)
                     /locus_tag="ACOIAF_000108"
     CDS             complement(77306..77755)
                     /locus_tag="ACOIAF_000108"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667145.1"
                     /translation="MFKKILLILMISLLGFGVIGCSDSKEDTNEVVKKETEEKLDEES
                     TSEDIYNKKLQFLNDTKNQSEIIGDEIRLLTEKYNNYEIVKSDWKEDLISKKKIITDL
                     KSVLEEEKSNILDGVSDFQKTILQNKYNEIIEYYDYMLKGISQNLNI"
     gene            complement(77844..81071)
                     /locus_tag="ACOIAF_000109"
     CDS             complement(77844..81071)
                     /locus_tag="ACOIAF_000109"
                     /inference="COORDINATES: protein motif:HMM:NF042814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HGZ2667146.1"
                     /translation="MEKYLFNQKLLKEKYNMEIDLDLYDYKQRRLILEKWRDIIDNKS
                     IDKLNEIQLKEIFVSDILNKVLRYKSLTDNIMDYNIKPEEKTKIDGTRADIVLGYFSN
                     EESDYRVTIELKKPTTNLDAKQLRKNNHNTPVEQGFSYLPKYGRNCTWLIVSNFKEIR
                     LYNANDATEYEFFSIKDIYEKDDVFKKFLYLLSSKSLMTKHGKSRVEMLWEENIKQEE
                     AIEKGFYDLYKNTRLNLFNDILLNNKDILDVKTCLTKTQKILDRVIFVCFAEHKGLIP
                     ENIFETCIQVGKKSVSRFGVWEQVKGLFESIDLGNQEKGINQFNGGLFAEDYILNNLK
                     IPNKCFENLYKLSLYDFNSDLNENILGHIFEKSISDLEELKLEINQVNIKKKDSKRNK
                     DGIFYTPKYITKHIVNNTIRRYLDEKKKELGEDKLPELKEEDYETVVVGRNIQHKRVQ
                     VVEYKTDKIQKYINFWKNYRDVVKNIKILDPACGSGAFLNEAFSMLKKEAESVNEILR
                     DLTDGEISFFDLDANILKNNLYGVDLNEESVEITKLSLWLKTANKYSLLTSLDNNIKC
                     GNSIIDNKEVDCAKAFDWNVEFEDVMMSGGFDIIIGNPPYVSTKMIPEKHREYYWKKY
                     KDLLISEMDLYELFLYEFCENKLKQDGFLGFITPNTYFTNKSFENLRKYLLKNVCVDT
                     ILDFPYRFFPFEDVNKETAIIIINKKLPNIKNNINLISINKENMKKIKIFNSNTYNSE
                     NKITIKSIINDLDNKIVIQSNPIILKMLKCKHKLGDYLNLHKGWMSVPNKTICDGIIY
                     DKKILSSNDIKDNNQLNGILNKCLEGKDIHRYYIDKVDKFVNIENMDDKTMRWHKSPK
                     IITQRIVGQNQNKIIATVDIEGTIIFPSGNIINLIKEDNLEDIYFYIGVINSSLINYF
                     YNKFYGESNTNLTADAVKNIPIPNLKNVDKSIVINNSKILINKYGKLYLYTNKFLKNV
                     FKILSIDNNPSNKLYNFYLLDFDEFYNELSKKKKLYFKNIDELEEYFNKYKDAINRII
                     YDINVCENTINTFVYDLYGITELESKSIEDICKTTKDTVVTSLAY"
     gene            complement(82566..83003)
                     /locus_tag="ACOIAF_000110"
     CDS             complement(82566..83003)
                     /locus_tag="ACOIAF_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667147.1"
                     /translation="MVFKSLDELVAYTKNKIAASMPEVGKEMKEIVKEEVNKQVYSDY
                     APSLYDRTYELLNAIDVSEITSDSVATEIKDSGSWFDIYSKAHAFPMERFENGGVWGH
                     GSTKYNPVFRPRTNIMEESNSKAQERVPKCFKSKMNELGVPVK"
     gene            complement(83004..83453)
                     /locus_tag="ACOIAF_000111"
     CDS             complement(83004..83453)
                     /locus_tag="ACOIAF_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667148.1"
                     /translation="MNKIQVDKLIQDEVRAIIPIIDENGKEEYIEVRNPDKETKEEIL
                     NKIWVGMENPDLALSQEDILKMLIDKLTNIELNIEIEDLVDGNISSELENVMYYIGQI
                     ENELTASLLMNTEIKLGQMKNEILQDRVLKETEEIEKMNNIKEKVVN"
     gene            complement(83467..84252)
                     /locus_tag="ACOIAF_000112"
     CDS             complement(83467..84252)
                     /locus_tag="ACOIAF_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail domain-containing protein"
                     /protein_id="HGZ2667149.1"
                     /translation="MVFLDDKFLFDNISSESMNIKLVTLGDDEILNEYGLPYEEAIKS
                     DSTFNKNPCYSEDETTIEPITLQFCLCSEHGEAYEWDDYTLENICNWFWQREFKPFIS
                     YDNIEEIYYLKARKIIKRYTKDKKGVLEIEFQPYTNYAYRSFQKVITVKDTREIKLNN
                     VSNVDEEYAPVIDIECLKEGDITIRNSTISDNEEDNLVISGLELNEKITIDNLYYTVL
                     NSSGENRFNIVNRKWIRLRRGVNILKFTGNCKVSIKCKYPIIK"
     gene            complement(84349..85164)
                     /locus_tag="ACOIAF_000113"
     CDS             complement(84349..85164)
                     /locus_tag="ACOIAF_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667150.1"
                     /translation="MRFAIKDASNIIVKNKTTGEPLFYTADLNAFNFKLDSESVYAKA
                     KGANTIAFDGAITASLALEQEVIQMPQLAMLLASDMVEESAKVGKRKLLTSDGTKKVT
                     LENVKPVTNSISAYSVEKDGISLVKKLQFTSTVTGSNTEITISTADFNAGDKVAVFYL
                     EELPKAKVIKIKEESTAPNYVIEADVMAKTADGEYMVLYMTIPNAKAQRSIELNLTAE
                     NPSGFNMTLDVLPDENKEYVVFAFVGDEDVSPVRMASMLGAELIDEKDVKSKK"
     gene            complement(85207..86010)
                     /locus_tag="ACOIAF_000114"
     CDS             complement(85207..86010)
                     /locus_tag="ACOIAF_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667151.1"
                     /translation="MLENYYITGLPIKLSEILGTIYQPTIEELIKFDMANLEIVNPFL
                     VLEKSYSQLCNEKTFELKCKYDAIPILDLMMLTSRKDSSEEIELLSDKIKKSLSILYK
                     TDIKNIEYMNKIKIGILIKFDDKKKNAFISREDFGLVSDLILEMFCIDKKNLFKDDED
                     KWIENTGSEREKQLIAHFKEKERKKREKEAYHLCDYINVVQNIDGYVPIDVILKMTYW
                     QLINAYKTKIQFKNYDESLGFAMSFKYQVEADKIKHWSKEIKIQTSTVK"
     gene            complement(86010..86504)
                     /locus_tag="ACOIAF_000115"
     CDS             complement(86010..86504)
                     /locus_tag="ACOIAF_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667152.1"
                     /translation="MAFPNKMISNIGTTLMSNQDFAKFMIYNNESQKDILSMPDIKNP
                     VKELRNKKVFFNRRVEKVLKEADVSVYITLFKYNLYKDRSRIIKTTNIEIGVVCHDEC
                     QDTFNGLRDAALVCCIVDTITENEEIAGIGKIKLESCSPMFNLDTSYNGFSIVVSSES
                     FGDM"
     gene            complement(86519..87508)
                     /locus_tag="ACOIAF_000116"
     CDS             complement(86519..87508)
                     /locus_tag="ACOIAF_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667153.1"
                     /translation="MSYLSDIKKRIGLGCSTPKEKRILQLRLSFKKYLKETPTCIEVP
                     ITDIDEVCINEDTKKAVVAINDITNNDKRALDEKNLLVESDLDVDVGCYLFYDNCYWL
                     TIFKEHKEMDTYKHFIIKRCNQFFNYKYKGQMYKIPIAVENLTLYSDGMADNKYTSIS
                     DTKRQLYFGSNPVTKTIDIDTRIMLTGKTVFRVTSINDFEYNGRETGADGLIKAICLQ
                     DALISKDDTINNIAWNDLSENDSVIIPFSKIIGYEVINLGEENEYIIDHFQGVKWLLD
                     KQYGYCDIIYQDEKRCVIQANTLAKYSGLEALLLAKDKDTNEIIDTKKITLRG"
     gene            complement(87510..88139)
                     /locus_tag="ACOIAF_000117"
     CDS             complement(87510..88139)
                     /locus_tag="ACOIAF_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667154.1"
                     /translation="MGTPLVKIYKKFLDAINDEEMLLLSNEIIEKMMYSYLEDAIVDF
                     NQCKKDLTIKYVDEKGEEIIPAAQLSYTSNYSNKNAEITLMGKETKEEYELDKDYTIS
                     FEDEKFIISFVVETTEEIIFKYKYLGEIVSDLDLEEIKILSYGMQIHWLQPKINREEN
                     LKQMLTDSDYNAKSGANMLAKLQAREEQLRTRFNKYQQRYMLKNFEGWN"
     gene            complement(88154..88786)
                     /locus_tag="ACOIAF_000118"
     CDS             complement(88154..88786)
                     /locus_tag="ACOIAF_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667155.1"
                     /translation="MARAKKENISKTEVKANTLEKDIKSTLNKKEARLNRLELQRKLK
                     QKKQEIDIEIKNISTWDVDYIDPREKQQIFSLSKVGSKDSTEFIDLDTLYRIVRRSPG
                     FFEEHRLIISDVDSLDVECTPMDIIDFLGLNSLYEHIRNPNEDYLEYFLSDKVDINSF
                     EKILNKNNVELTRRLAERAIDLHKKKKFDSGLKAKLLAKRIGIEDLYLFS"
     gene            complement(88839..89849)
                     /locus_tag="ACOIAF_000119"
     CDS             complement(88839..89849)
                     /locus_tag="ACOIAF_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667156.1"
                     /translation="MATAKTLKRIAHELYTDTFREYTDRDGNTITYRDAESAIKNKLK
                     EMMGGEKYNYYKFQENKWGFYNLISELISDDINRLNEEVFSPFCDFENFDLGDKKEFT
                     VENTNLFKVANIADGVNSTRRQRLLNKKVPTTAFKLSVAIYEEAERFITGRINWVVFV
                     NRVSDSYHYDIARRIAKTFEGAYSTINAKFQATGNSDKVLLELIAKVEGATGKKPIIY
                     GTPLALSNLEGIQTDLEKEEKRKYGFIQTFRGGYKVLSLPNAYDENAAEGKEWALDNK
                     AIYVIPDGEKIIKLGTEGDVLVIENTDEKERDDQQIEYFMAQKIHLGVVTAAKFGVYK
                     IQ"
     gene            complement(89937..90434)
                     /locus_tag="ACOIAF_000120"
     CDS             complement(89937..90434)
                     /locus_tag="ACOIAF_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667157.1"
                     /translation="MKKDKAIIQTDKVKNPDVLTGKYVVPSEDTNPINLENGAVINIG
                     ELEDTEYGRDTHKIYKVTNDTLDWGIVDDPATMYDERLDERDYEVSPGQICRCRRLKK
                     GDEVTISLLHIADKSIAVKDKLQLKSDSFQFEKLPTADAKTPVAEVLELWNYEGQDSV
                     TIKVL"
     gene            complement(90449..91882)
                     /locus_tag="ACOIAF_000121"
     CDS             complement(90449..91882)
                     /locus_tag="ACOIAF_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667158.1"
                     /translation="MKKIKILKLPCNLKHYSDKKKLEELNSNLLPVYIYVMHEGTNPN
                     GTKFYEEAIDKAEPTLKNVPILGYVKINEDGKYDFDGHNVLTQVVQTDEGFILEEYYE
                     ERIIGVIPETNKYEKVEIDGQKYVKCKGYIYKSYSNHAYDIIMDSDEIEISMEIDIND
                     YQLDDSDGFYNIKDYVYHGITCLGSDVKGAMGSNCCLTKFSRKINYKEEISKICSEIY
                     ALEHGEGEKNLPNKNQKLNKNSEGYALAVSNLSTEIRNKLKEYKVETENWYGEKVEVQ
                     AFYYNDLIPEENIAIVEDEINWGYYYGIPYIVNEDAVILDYENKKSYIQTWREKQEGE
                     IVQVFSRQEKLKKEIIEKFTEKQKEISNLKASLEPLQAFKEEKEFELFKSKVDDVAQK
                     FKLAEDEIKDIKIKAYNKEITLDEYKKELGYIFALKTLSNKQSEKENFSINDDKNNTI
                     KIPISNNNDSFSEPEEISFIKKYSDKE"
     gene            complement(91889..92080)
                     /locus_tag="ACOIAF_000122"
     CDS             complement(91889..92080)
                     /locus_tag="ACOIAF_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667159.1"
                     /translation="MSKFIYAFSEDDKKLLMEKGYRFICENKLNNKTLYVFENKSKLI
                     NNFSNEEMKRFIFTSKIRF"
     gene            complement(92083..93561)
                     /locus_tag="ACOIAF_000123"
     CDS             complement(92083..93561)
                     /locus_tag="ACOIAF_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667160.1"
                     /translation="MGKKNNNKSKSKDNNSTQNQINMLNAQIGKYASVKESTTLATEM
                     YKLRSISRDKLRKALSNPYQISNTDILQDASMILKATSGTYRRVLNLISNMNTFDHIL
                     YPKDISRLKTKEKITKAYMNSASQLEKYNIKFTSAWITEKVLELGEVYLYKIEDNSGM
                     VLQQIPAKFCMITMIENNVMRYAINIKKLTDKNIISFPEEIKNIYKKYKSNLLTREEL
                     IDNTYFQLSDKAVAFNYDLDSVKGVPFFCFIFDDLMELEDMKDLKSTNAVIESIKLIH
                     QKIPFGKNDEPLVDLNLIPIYHNSTKANLPKGTAITTNPLELETHTLSDGKSKINDYV
                     KEAKEFIFDNAGINTALLNSDKINNESILNGIIADSLIPMRIQQMIENWVNFELNKDK
                     KANLFNMKFIGTTHFNKMNLSKQYREDMGYGGSKSLFIASTGFTPLQAINTLQAEKLM
                     GFDEFLIPQQTSHTQSSGRPDKSDIGTDNGNSTQAKGNGEND"
     gene            complement(93578..95326)
                     /locus_tag="ACOIAF_000124"
     CDS             complement(93578..95326)
                     /locus_tag="ACOIAF_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667161.1"
                     /translation="MRTKYEKRELSSNDSSRNLLESIGEYWGAFYLANPHRFCMDYFG
                     FNLHLFQQILIYMMMKSDQFVFIASRGLGKSWLLGVFCCVIAVLKPGTCVLIAAKRKK
                     QAKLLITSKILGDLYLKSDTLKREIKSFQVNAQEVSIDFWNGSRIEAVVSNDDARGYR
                     ANVLIVDEYRMVDEGTVNDVLVPFLTNPRQPGYLQNPKYRYMQEENKEIYLSSGWYSQ
                     HWSYKKFMETVKGMLSGEDMFACSIPFTCSLEHGLLTKKRILKEMKKESMSDASFMME
                     YCGVFYNESDDAFFKSSWVNPCRVLESMFYPPSDIEYLENKKKRDKKYHLNKIKGEIR
                     IIGADIALARGVKNDNSIYTLMRMLPNEGTYKRCVVHIEAYNGMEAEKQAIRLKQLFS
                     DFQADYMILDTQGIGTTVWSYIQKANYDSDRDEWYDAYTCFNEDNTVDKSLAKKSLPV
                     VYSMKAYADENHKMAMSLRDVLTNRTLELPISDIEAKEMILEKEMIKADEIDKKAELE
                     AKYIAPYLQTTALVNELINLEYSADGGKIKIKEKSGARKDRYSSLAYTNFLADYLEEK
                     EKRKNRNNQKTVMIYW"
     gene            complement(95316..96215)
                     /locus_tag="ACOIAF_000125"
     CDS             complement(95316..96215)
                     /locus_tag="ACOIAF_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667162.1"
                     /translation="MAISKTKCLNCGEEKYTDRYFWKSHSEIFTLNKRLPVCKECFRA
                     RFLLLNGCYNGELVKALKHICFNFDVYFDEKLAKELADKKNKDELIDEYMKIINRNSK
                     YKGKTSLDNLLKEIYTGNNNDKNIVINDEIKLKWGRGFDDYEYKILERKYKEYKEYYE
                     PESLTERKLFEEICIIELEKDKSREKGDMKAFNDLSKLVSSKMQDAEIKPSQKKKAGD
                     SQDDTFGMKMMIYEKNKPVKDRLKEYEDVDGFEAYVNKHMKKPLAVALGLATGKYSIN
                     DGDKDIKFKDDVRDILEGNKNED"
     gene            complement(96346..97545)
                     /locus_tag="ACOIAF_000126"
     CDS             complement(96346..97545)
                     /locus_tag="ACOIAF_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HGZ2667163.1"
                     /translation="MEDILKKKEDESLIDYRIRIRLAKVNKEIDLDWGEIVELLGLEC
                     SPDHCRKVSYGLKEAFDYLNSKVQDNSTQEEIDKINEKILELKKIKVQLSDERSLVNK
                     KIREYSRIDNIIDLFNNKIDDISLHKPFLSDSSYKCYESSNQEAIMLISDIHYGLETI
                     NAFNRYNSEIFKIRIQYLKDKIIEYSKLHKVKRLHVMLLGDLISGHIHNSIRLENREN
                     IVEQIIEVSEILSEFIYELSKEIDKIIVYSVGGNHDRVLPKKDENLDKDNFTLLIDEY
                     IKLRIKSLGNVIFQENIYDNDIIVAKICGNTCFAVHGDKDKMSTAIPKLTSLIKLIPD
                     YIFMAHLHNCKEDNYGESEICVNGSFSGTDSYAKNLRLSSHAMQKLMIFNESGRICTY
                     NINLSKL"
     gene            complement(97622..97810)
                     /locus_tag="ACOIAF_000127"
     CDS             complement(97622..97810)
                     /locus_tag="ACOIAF_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667164.1"
                     /translation="MKNNKGTILEKCEVIKQEDGTYKFIEHTKNGDNEVLSSDVFDQY
                     IGKTDLNITIRLSETIVK"
     gene            complement(98183..98800)
                     /locus_tag="ACOIAF_000128"
     CDS             complement(98183..98800)
                     /locus_tag="ACOIAF_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667165.1"
                     /translation="MVKYNVHKALAEKKLLEDKIDRCISNFKIVGTKKGSDKNVYETK
                     TSVEDFNVEVSSRYQQIEDLIYNYNALDKAINISNAITNVQIGNKNYTVLEAIKRKNS
                     IELDKSLLRQMVSNYDCMMSEVNRRNEEVQKNTDRMFEEKEKSKDGAELISFYKKQQE
                     WSLVDPLKVREKIEKLRDEIEEFEKEVDFALSTSNALTIIDVDLK"
     gene            complement(98856..98946)
                     /locus_tag="ACOIAF_000129"
     tRNA            complement(98856..98946)
                     /locus_tag="ACOIAF_000129"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(98911..98913),aa:Ser,seq:gct)
     gene            complement(99514..99792)
                     /locus_tag="ACOIAF_000130"
     CDS             complement(99514..99792)
                     /locus_tag="ACOIAF_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HGZ2667166.1"
                     /translation="MKKVDFLKEVAEELGVSQIRVREILDVIEKKRNEVLQDGEELTL
                     MGVKYLTKIQKGKEGEITLKDGTKKPWKTEEKRVPKLKIAKSLKDAII"
     gene            complement(99926..101368)
                     /locus_tag="ACOIAF_000131"
     CDS             complement(99926..101368)
                     /locus_tag="ACOIAF_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667167.1"
                     /translation="MIYNNNNFYEEIDGKSAQDYIKNLDYSIDNSKDRIKYIEERLGV
                     KHTWFEESNRNYIDTFYNTLTKRNENIITRVKYQEKKQFNDKFWENIFEQTSDSPLDK
                     DGVYYVTIDGEEVVMDYNRFITWCGYNQINPIEYINVKNPFSNSNGTWEYTHHNTSKI
                     KLILNKDDSIYSTSNIAKTLEIMGSYILSVDDKPKETKIRIYNSKELFDRACQEEALL
                     NKIAPANGGNINSNTSNKDGFLEDNSFAFFQLPKNYKKIKDIKVKPKDIKKYPIIKTY
                     SDTYEWYKAKYKELGHKQLSKEELKLKRMVRKNLNALKNDMTDVKNSIERPIIWKAPL
                     ADAGSPEWDYLDMFDKSHVKELLKIQKGNDLQDDLTCIVMDLNNIISKIEFTTVQKDI
                     LNLYRKDKSLEFISNLMNITPQAINNQINKIVNKIIDAYEKNYEENYYYISVCKGKYK
                     KCSRCGKIKLLSRFDKNGKKGYRSNCKNCN"
     gene            complement(101371..101619)
                     /locus_tag="ACOIAF_000132"
     CDS             complement(101371..101619)
                     /locus_tag="ACOIAF_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667168.1"
                     /translation="MKEKKIELIYLEDLDESEKTGVHKNRYQILNEEYEQMIDTYTEM
                     SKRSSYDIVNERHKEDFKDELWDYDDTDYDDGLQGKEV"
     gene            complement(101797..102660)
                     /locus_tag="ACOIAF_000133"
     CDS             complement(101797..102660)
                     /locus_tag="ACOIAF_000133"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667169.1"
                     /translation="MKRGEVLEIGKRKKSFSPEQRAIINNKKELPYHNFKLGGFVFCT
                     YVKNELLFNEVNISKANISRLIYLATYLNYNTGEENLLVRYSQFKEMIPIDRKNMREI
                     LNLKDRAFRNFLADVKKCELLYESNNKFYINPKYFTKGIPNFENKEYTRIYINTSRFL
                     FEHCTPRQHKQLSYIYQLIPYIHFESNILCLNPHETDISKIKKISLYKICQLLNISTI
                     KQDMNKFKKELLRFYIKVEDDKYYFFKYVIVEGNNKNTDYFIINPCVIWKGNNLNLAK
                     KTIDLCFFNCI"
     gene            complement(102932..103204)
                     /locus_tag="ACOIAF_000134"
     CDS             complement(102932..103204)
                     /locus_tag="ACOIAF_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667170.1"
                     /translation="MKKSSSFYLEEDIFGEIEKYQNDKNISSRNTALERIILEWKNLQ
                     EENKLLKQCLGNGVYRTNKKTNVTEEKKDIKRENPIIKNIFNNMPD"
     gene            complement(103217..104131)
                     /locus_tag="ACOIAF_000135"
     CDS             complement(103217..104131)
                     /locus_tag="ACOIAF_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667171.1"
                     /translation="MEENYQVIGLDIGRGYVKGYSKYNGMVKECLFKSVFGDGRNIDF
                     EKYENPIYIDFESVSYFVGSLAEKESITPIRNSDDSKVSFTMRILVAAALNEIAVADE
                     VKLMMGVPYKSFRKTTLKEVVDTYKGKTFKVKDKIKGGHKEIKISDISIFREGDAALF
                     HTLEGKVNEDKAVGMVSIGFRSTEMSFFEKGFVFNDKLSDTLEAGNQDALTMVQKQLK
                     DRGIIRELNEIDSSNDYDELKKVAYIMASESTAQRISSKWKNIDEMDVYVSGGTALHM
                     TFDNRFKVSKDAQMATAKGLFEVGMEQF"
     gene            complement(104553..104741)
                     /locus_tag="ACOIAF_000136"
     CDS             complement(104553..104741)
                     /locus_tag="ACOIAF_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667172.1"
                     /translation="MESGLFVSRILCVVLSFPHFKKIADGFTDEIALYFPHKTNIYLK
                     IFLRFLLLNYILNKENIK"
     gene            105237..105395
                     /locus_tag="ACOIAF_000137"
     CDS             105237..105395
                     /locus_tag="ACOIAF_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667173.1"
                     /translation="MRITKGKIKELIELCHQLEKLFVQVFKSLEHILISLCSCIGWLL
                     ILIYIIKH"
     gene            105648..105839
                     /locus_tag="ACOIAF_000138"
     CDS             105648..105839
                     /locus_tag="ACOIAF_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGZ2667174.1"
                     /translation="MKLEKLIDIRKEKKYSQEDIARMLDISLRNYHRKEKGENQFTVS
                     ELEKICSYLDIESKELLDI"
     gene            complement(106053..106364)
                     /locus_tag="ACOIAF_000139"
     CDS             complement(106053..106364)
                     /locus_tag="ACOIAF_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGZ2667175.1"
                     /translation="MNRLRELRKEKGYSTQQVGELLGVHYVTIQNYETNRRKIDNETL
                     IKLSNIYNVSIDYILCLTDNRENIQLEEEEKQLLNNYRELDTKSKTIVQEQVNTLKKL
                     L"
     gene            107096..107419
                     /locus_tag="ACOIAF_000140"
     CDS             107096..107419
                     /locus_tag="ACOIAF_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667176.1"
                     /translation="MDEKILELLQKMDTSINDIQGEIKWINTRLDSMDGRFDGIENQL
                     SDLKEGQEEIKKKLDLTYNQVARNMEGITEVGEKIDTLKNDMNFVEMATSKNWNEIAK
                     LKSIK"
     gene            107431..107664
                     /locus_tag="ACOIAF_000141"
     CDS             107431..107664
                     /locus_tag="ACOIAF_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667177.1"
                     /translation="MDEKTLELLREIQESIKILNDKLDEFDYTQDKIKSNVEGLLECF
                     SRIDFRIEDLENGQTSLYTKLDIVQNETAKSIK"
     gene            107759..107947
                     /locus_tag="ACOIAF_000142"
     CDS             107759..107947
                     /locus_tag="ACOIAF_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667178.1"
                     /translation="MYEQRKAKVIFNKGAGNSRGESYTNRVTIPTTWIKHMDITKLDR
                     EVLLTFDGNKIVIEKIDE"
     gene            108177..108371
                     /locus_tag="ACOIAF_000143"
     CDS             108177..108371
                     /locus_tag="ACOIAF_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGZ2667179.1"
                     /translation="MNLERLKELRLERGYSQQQFATYIGLSTSTYTDKEAGKTKFKVE
                     ELEKILKFLNLDPLELLDLD"
     gene            108523..109236
                     /locus_tag="ACOIAF_000144"
     CDS             108523..109236
                     /locus_tag="ACOIAF_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HGZ2667180.1"
                     /translation="MLNIYITNLGKYNEGELIGEWAELPVNEEELQEILDRIGINEEY
                     EEYFITDFETDIEGLEISEHSNIEQLNELAMKLERLEEYEIEELKALLERGYIDFYDM
                     LEGDICRLSGNYTFIQLDDRSPLDDENLGRSYIEEICCGDLSCIENIAYYFDIEAFSR
                     DLRFDRDMIIENLYEEDIEYYENMTDIEFAENYIERLGDIEELGQETLERYFNYELYG
                     TDLRYENICIASNYLAIVS"
     gene            109304..109444
                     /locus_tag="ACOIAF_000145"
     CDS             109304..109444
                     /locus_tag="ACOIAF_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667181.1"
                     /translation="MKKLIIKIKSLFKKDLDSSYWSTTESIILKCGNSNQLKLLKQSN
                     KL"
     gene            109461..109682
                     /locus_tag="ACOIAF_000146"
     CDS             109461..109682
                     /locus_tag="ACOIAF_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667182.1"
                     /translation="MKINSKISNVVWDIKTKELNKQDILFIENFNPTIYHNDVDCYLW
                     SLDEKLPSGVWLENIKFNNEITEAKAIVL"
     gene            109727..109963
                     /locus_tag="ACOIAF_000147"
     CDS             109727..109963
                     /locus_tag="ACOIAF_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667183.1"
                     /translation="MINYSKYMTATDLINICDILEGETIGEAGTYNSLSTRLGNLIGL
                     DWALKGEELRQQLIQNINNMMYLDNENKIQYKEF"
     gene            109998..110501
                     /locus_tag="ACOIAF_000148"
     CDS             109998..110501
                     /locus_tag="ACOIAF_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667184.1"
                     /translation="MNKIIVFYDIEGYPVFQILVRNVKKYTRQNRIFKLVDSFIKKNI
                     NNINLNDGKIYDSSSWIIFTISKTGHYKLKLNLDIDYDILIKKLDIKKSKRESNSKTE
                     RELFYIGTGWCKRKEIYNLLTRQNQIGYEDYDTLTIDGDVYIRTYNEKCRTILLHSNY
                     QKILKEI"
     gene            110539..110901
                     /locus_tag="ACOIAF_000149"
     CDS             110539..110901
                     /locus_tag="ACOIAF_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667185.1"
                     /translation="MNKYLIGCIGNCLRSMKAIKTRFFLIDNNNIEDVTLKLCKGVEH
                     IAPFRYKYGLANYSFGCGLSHYLDTYNLNTVNGLDLNNTHLIMLTLNDLKNIVGCKTV
                     FDIDVEEKLLNYIKSYYR"
     gene            110952..111215
                     /locus_tag="ACOIAF_000150"
     CDS             110952..111215
                     /locus_tag="ACOIAF_000150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667186.1"
                     /translation="MLINKAKGIKTSLETSSLRELKGYIKDLRKALSGYKYKNKIDEI
                     TEYQIDKLCYKIDNTDIFDLKYKEIEKYIDNLILIINFIFMEM"
     gene            111253..111837
                     /locus_tag="ACOIAF_000151"
     CDS             111253..111837
                     /locus_tag="ACOIAF_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667187.1"
                     /translation="MNEYILINYTKGKGACDHCGRTIKNIATIKNNVTGEVFHVGLTC
                     VEKIMKLNVTFYKALSREIKKHYKCMEFYSKGLDIETNLNKIVKNNTKYKEGSYAYKS
                     NEQMLEDAIAEVAWSLARMIDSCMRMNKLSKSGLIDIDILNSLFIKYKEYMSKFDENY
                     NKNKYKYKSCYTMKPLKVILDENEDLKELYKSMQ"
     gene            112155..112640
                     /locus_tag="ACOIAF_000152"
     CDS             112155..112640
                     /locus_tag="ACOIAF_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667188.1"
                     /translation="MKFKNIAMGMLLGLIIVGVAGATKVKAAELDNFYVETTDRVVEI
                     LEDESVVLYDTKEQVYNFYPVCLGDWNYSFKNKKDLDRAVATYKELSNNISHSKDVYV
                     INKLNNNGNIKVFLSDGSSIVYIKNDNKYYFYPSCMGDWYLTLDSKVDLINCIKFYIG
                     E"
     gene            112905..113987
                     /locus_tag="ACOIAF_000153"
     CDS             112905..113987
                     /locus_tag="ACOIAF_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667189.1"
                     /translation="METLLVKNLLKEYKLWQGGEHRRIYINKVPSKIDFKEFIKNNDF
                     GIGLGEGLGITKIAQRVENSGNISELKIYYDCNNKNFYFIKQYSNVLKKFIDLLTDEI
                     IEIYSKEIYREDNVSQEIKENSFNVLTLNGSEKQVELANEIRDNVLNFVDELEKIKMK
                     KIEDGDYKVKDKSIEEMTEKCKRKFKRIKEEMRSIESAKFFIDNFKDILEHNSLEQKA
                     FHINQTLKDSQFAEESKIWSLLEYETQVPHKLKKVKDYNISYEEAKKIAREIKFNNAW
                     ANEIKENVLKIVEIFDEIIKQVPNASTEKTKELIIETLIQENPRYFINGFKEIKESKV
                     ESIRKIEEISRKIELIWHLFYRGVNC"
     gene            114064..114570
                     /locus_tag="ACOIAF_000154"
     CDS             114064..114570
                     /locus_tag="ACOIAF_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667190.1"
                     /translation="MNIDFSTILDLKVNKENVKMIEKEINKINNDIFDISLKDCANCH
                     VGCVKETAGKSLKIETWGHGEINSKIGIRLRFNFYYSKSDKVDIENIENILIYVNSLC
                     SKNVESDLIANVRPKFYKLEGKYSTRVEKCEIRHELTGINKQEIKEFLEYLTEIEKSE
                     RTFIYKNN"
     gene            114589..114891
                     /locus_tag="ACOIAF_000155"
     CDS             114589..114891
                     /locus_tag="ACOIAF_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667191.1"
                     /translation="MENKIIEFLEEMENLKGQEFNIIEFDNEIQELYARAVERTSSWD
                     FFTSGDINEYLNVGSYTYCGLGEDYDLDINIEFEVVEKNEEDVCSSIIKIINIEKL"
     gene            114906..115238
                     /locus_tag="ACOIAF_000156"
     CDS             114906..115238
                     /locus_tag="ACOIAF_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667192.1"
                     /translation="MQKYFKEEWHYIPDFRFKIENIENCTIKVNDLKVDMKMSQDFRF
                     KGYIEYTIYIPYNHKIFTREYDEILDLEQPSECEQFCDELSELRGKVEEGIEQYLKDK
                     NKLVEIEY"
     gene            115299..115847
                     /locus_tag="ACOIAF_000157"
     CDS             115299..115847
                     /locus_tag="ACOIAF_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667193.1"
                     /translation="MNYKKIKRFLKIIDKNIDKIEEEAIKVYKESFLAEDNTIRIYIN
                     LEGKVEAISVHNSLKYVEDIYEREIFICELNKEKISVNELLGEPCYLNDYEEFEKWCK
                     IKNEDLDWESYKKFNKDSFDELIERNLEDSLSDFSEEIKKNIENRKQELQVIVERSIE
                     NYYYHDIILFNNLLNNNSLNNN"
     gene            115977..116498
                     /locus_tag="ACOIAF_000158"
     CDS             115977..116498
                     /locus_tag="ACOIAF_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667194.1"
                     /translation="MFKIEVGKKLEAEIPVGLSINFSETGFTAIFKLDNLSDKEIIEF
                     RKGNLRIDVSFLDKIIFFVFTNTMGIGDADIPFTIHLSKCKEFQELEENEGYAMDLML
                     VEANNNIVKGFRRVGLNTNTSKYLKKCAMEQLEYDFNMDEHMRRVKEIQRKYNSREIK
                     KLSGAYSKFERKQ"
     gene            116675..119029
                     /locus_tag="ACOIAF_000159"
     CDS             116675..119029
                     /locus_tag="ACOIAF_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HGZ2667195.1"
                     /translation="MKKELLAGSCNLNKKEQTLVGHIKDLFKVLESILDIDLYSGKDI
                     EILKICCVLHDLGKINLITQQKIEINNKISSSSLKEEIKKLEFEKKSLEKIAKHNVFS
                     GAFLKSILEKMNLSEEDEFYIYKSIMLHHGNYIDYMKLTTSKVQEEIYKYIDDGILEN
                     KESFNLKDIEDCISEILNIDFKFNEDVLDYDYLDKLSEKMVIESDYDNGQIDDSILNQ
                     RRAKYILYKGMLNLIDHVAQGTNKDIKFYNDFTDKEIDEMILNDIYNNHDNLEKNNIE
                     FNTIQKKMRKFSEKNVLTVAFTGSGKKVADCRKNFKRKLFLVPNQFSSESFYIKNIFK
                     NDYLGLLNGDINLYSYSENYNEQDCEVTLRDIDLSLNLCKPYLIATIDQLLLSMFKFP
                     GYEKIFAAVKNASITVDEVHLLNPKMFLILIYFIQFACKYLNVDFHLMATMLPKSYKE
                     QMISKGIIFQEECNENITDKGEIVFIESNKEEDICEGKNVKVSFIKEKEIKSIVENAL
                     EKKQRILIIKNTIDSANSTYKLLKENLSNKYNDADINVLHDRFKFKDKEEKYNKIFDE
                     KGDVWISTQEISLDIDFNIVISDLATMDSLIQRMSKCNSNDKCEYGNFYILPSKDEIY
                     DDKLKNTTKSILNKILKTESIFTMSIRKNILNEYYDNSVVKKYFEDSFINCDNEIKNI
                     FGMYKADFDGLDLIFNFEPYKHIVDNESEASKLFKATDTNCKIILEEDFYREDIDLQR
                     DSIQVSGFIFNRLCYLKLINKLEGYMVLKSSNKLKYNSTLGLIL"
     gene            119214..119786
                     /locus_tag="ACOIAF_000160"
     CDS             119214..119786
                     /locus_tag="ACOIAF_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HGZ2667196.1"
                     /translation="MTWNDLKNNDITYIVEYINSKLNDSKSLTKVAIELGVSESSIRK
                     YLTKRGYKRINDEYVFIGDNSMTKVVNNRQQSNNDNTFNMTIEDDNNHNIVIDNQFKN
                     NIISLAKDYDKIQNILNWFENKEDTNVIEVVQDGIKIDLTSKDAIRTTVRLNKDAWNL
                     FDEFCEKFREFNKSDLMSMALLEYIKKYDK"
     gene            119919..120845
                     /locus_tag="ACOIAF_000161"
     CDS             119919..120845
                     /locus_tag="ACOIAF_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667197.1"
                     /translation="MNYNLKNENVLSELLQNEDEKTRIGILKVYKVLIFWFAKEGKLV
                     EEENDYIVEIVGNDKEEIVRDMSQNKTYNAIMAIIFKINKIFKTLNYDIHFSKPKNLV
                     AEFDLSKYLTQDEVLQVCSKLTNAQDKFIIYAAFCGLIGKDAEDLRGIKIKDIDLEKR
                     LIKLKDTEFKLDKFLFDFTKDTLEQKEYKQYLEQDSNAAEFYLFNSNSEYLIKTKPDA
                     RYGNGLKEMGYSTIRKRLLIINDYLDNKVQLNFTNLKISGIMHQMHLNEKKYTQFGYW
                     TNKSLKEFKEDNNLNFKIQEVLSIYKQKYIKE"
     gene            120850..122418
                     /locus_tag="ACOIAF_000162"
     CDS             120850..122418
                     /locus_tag="ACOIAF_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667198.1"
                     /translation="MSKDVKENISNEQKDLNKINLIYHIIYDNDRFKMITRKYFEIFR
                     LKGMSAEEISLKFEEFLYSLISDKFKKINTVNDVKKLRNQENSSSLKLKKRWSDMTLD
                     SLSEDTKSSRKKSSKYLKKELEIVESEYLKNGRIISAGVKNPKELKEQRKDFLDEWKS
                     SSSYNICDYPYLQELSTSKINSAFIHDILMCITEVLKDDYDFDINKIAIKTPPHISPG
                     IFLPFTKGKRYSHLNVKKNENEYMSDDYSAKNLSGKQMNFFYKFSIGQDENIENLKLE
                     LINPDTEKRALPKIYLDKQDLEIIRFVYTYSHMNSFSFYLGDLVKYLGLSDGKKNYIN
                     IKNRLLKLPYYTFYSNQTNDKGELKYEISFNLFSSTTIVNDENNNNRELVKITKSFIE
                     EVERVNTDIMYRNELEKLQLTQSINLAYFLEGRRSFLISCGEDIKNKSFRYDIEDLKF
                     DVHLNKSKTVKQNMEYLELGFNEIKENQFIIKDYKRGNSYFDVYFYEDFEKRKRLINN
                     TILSLPDYMIENKS"
     gene            122892..123158
                     /locus_tag="ACOIAF_000163"
     CDS             122892..123158
                     /locus_tag="ACOIAF_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain containing protein"
                     /protein_id="HGZ2667199.1"
                     /translation="MSLKYKDKEFVLVEKKNINELDNSNIKYIDLKDKQYYVVTQGKK
                     PKRFNLEDIKNIKKDLDNGISLRKCATKWNCSTRTIQDIKNNIY"
     gene            123234..124412
                     /locus_tag="ACOIAF_000164"
     CDS             123234..124412
                     /locus_tag="ACOIAF_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667200.1"
                     /translation="MQELKPVARISKSKLKDILDVFKTSIRKVEKIDYSACNSCAIKN
                     NPSFDSHKLNEFCSNCPKLKTISEFSYVTEKETELGTIEIVTNESKCMALSKSAIKQY
                     LAYHFIVSNKNYVRKAVSFQNIADICNVSIVTARHNHNVLLRVGLVYSTEIKRGKFDI
                     VIDEEYKNHLKKEDGGKGYLTMSLDTLNHLLSFENVNELKLEIKKLIWADAKGSKLGK
                     RIRFKKDNLISILPEYIKKSNKRTEAFLRSSKTLFDVSNDTIDTKRYETKETISEKYT
                     EPIRIQISKFFDNTGCSLSSNYSKVLSQINLDRYNLFEEDLRSKLDMEKDIIINDLVG
                     LSVQYGVTRVLAALDYMFNKYTVDEEDINYNEIRNPGAFIRTLIVQSINKNGSLAIAI
                     "
BASE COUNT        46369 a        15977 c        16943 g        45351 t
ORIGIN      
        1 aaaaaaaaat actaatacat caacaagatg ttaatattga aatttatatt ttattttatt
       61 cattacaatc acttcgattt atagtttttt ctgactttct aattattgat tttaattatt
      121 acttttaatt tttgttttaa attctgaaaa acatttatta tttatacttt aataaattaa
      181 aagataaata atatcgaata tatgttcttg aaatatcgaa caaaaagagt tataataata
      241 atgtgctaaa aataattggt atatatcttg tacacataac aagaacatat gtttgcaaaa
      301 cggttaattg tttatatttc tatctatttt ttatgtctta tactataaag ttaaatataa
      361 aattaagata aatattatta ataataaata gtttgaaaat tacaaaaata tgtcgataac
      421 tggtaaaaaa tggaagaact acttggatag aatgtatgtt acaatgtaat taattaagtg
      481 taaaggggaa gtatttataa taggtatact ttatttttct aagggggaaa gtttatgtca
      541 gaaggaaaaa ttaacaaaga aaaattaatg aagctaggta atatgctgtc agaattaaaa
      601 aggaataata aagataaatt tacaaattat aaatctgaag ttgagaaata tgaaaataaa
      661 aaaagaggag cataacatcc tctttttact tcttattttc ttcctctaaa aaaacctcag
      721 ctacattgta taatcttttt tgtgcttctg gacttaattt acttatcatt cttaacattt
      781 ctttaatatc atcatcaaaa tccatatcta aagttgggtc ttgaaatata tccccttctc
      841 ctgttcttaa ccaattttcg tttacattaa attctttgca tatatcattg attattctat
      901 ctgttaaatc tctcctacct gtttctagag aggaaatgtg gtcttgagat agaaataatt
      961 tatttcccat ctgtgtttga ttcagcccta attcttttct taaagttttc aatcttttat
     1021 tcaatattat cacctccctc tttaaaatat attatcatat aaaatatgtc tttgcaataa
     1081 aattgatata aaatatgtca aaaacatatt acctgttgac aatatgctaa tgacataata
     1141 taatttaaat atggaatgtc aaagacataa aaaggagtga taatatttgc ataaaaaaaa
     1201 gatgaatcaa aaagagcttg agttattagg gaaaatgttt ttaaaaataa aaaaaataga
     1261 taaacagaaa tttggagagt taaaaggttc tgttaaaaca tcgcttgaat atgaatatat
     1321 tttagttaat aaaattagat aagtatacat acgtacattg aaaactgaat attcacacaa
     1381 ataaaaagtg caaccataaa ttaacaaatt ttataattgc acttcttcat ttatttaaaa
     1441 catggatata cttagtttct tttttataaa agagtagctt agctactctt ttataaatta
     1501 ttttttagaa ttcagtaact tattttgagt tttaagcaac tcttttgcca ttaataaaag
     1561 gtattctttt gtatcagaag ttaaagagtc aaaaatgtta agaaattctt tttcgtatgg
     1621 agaggaagca aacatatctc cctctcctgt tcttaaccaa ttttcactga caccaaattc
     1681 agaacaaatt aattttatat gtttgtcact gaaagttctt ttattaactt ctatcattgc
     1741 taatgttgat tgacttaagc ctatcttttt tgcaaattct gcttgattaa gtttcaattt
     1801 ttctctgatt tgttttactc tagtatacac atgtttgccc ctttcactta ttttgtaatt
     1861 aaataatatc acaataatat tacaaatgca attaaaatat aaaaaataat attacaaata
     1921 aaatataaaa gtattgactt taacttcatt tgtaattata attaaattac aaatgaagtt
     1981 gagaggtgat gtaatggaag atataatcac aactgaaatg aagaatacat ttttaaattt
     2041 atcagtaaat aatcaattag aaattcttaa tcttgctaag actattaaaa aaatagaaga
     2101 tgctttaaat attgaagaca atgagaatta taattaaaaa atgaaatgat taaggagagg
     2161 atttgaattg aataatttaa gaatcaaggg aaaacaaaaa tttatagatt tagaaattcc
     2221 tattatagaa ggaggatttg gagaaagtaa aaaatgtatg acagacaaaa ctatatctga
     2281 aatacataat atgagaaatg ctgatataag aaggagaata tctgataatt taaaaaggtt
     2341 taaaaataat attgattata tagatttgaa acgtgtgcat gagacgcaca cgttgataga
     2401 attaggttat tccaagcaat ctataactca agctgataat atatttttat tatctgaaag
     2461 aggttactca aaactaatta agataatgga tactgattta gcttgggaaa tacacgataa
     2521 aataatggat gaatatttca atatgagaga acagcttaca gatagttata tgattcaaga
     2581 ttctattgaa agagctaaaa gatggataga agaagaaaaa gaaagattat tacttaaaga
     2641 aacaattcaa gttcaacaac ctaaagtaga atatcatgat aaagtattaa atcctgacaa
     2701 attagttacg acaacagata ttgcgaaaga tttaggaatg acagcacaag cattaaataa
     2761 attactacat ttaaacagaa ttatatatcc agaaagaata aatgggcgaa taaaatgttg
     2821 gaaaccatat aaaaattatt tatggttgat acctgaatat gcagatttta aaataacaga
     2881 acatgatacg cagcttaaat ggactgagat aggtagaaaa tggttaatag aatttgtaga
     2941 gaacattaaa aaaataagtt aaatcaagaa gttaaaatgt accttgaaaa gtagatattt
     3001 aaaaatataa aagataggtg attaaatgat aaaacaaata ctaaataaaa gaggtagtct
     3061 attaaatcta gcaagtgagt atttgacaga acaagactac ctcaagtttc aaggatggta
     3121 tacacataga tatagtaagc tggatgctta tgatgcttct ataaatgaat atttaaatta
     3181 taaaaacgac aaaaaattaa taacttatgt atttaattta aaatttcaag aagctctcat
     3241 agaaatataa tattactaaa catggtgatt gaaatgaatt ataattatgt aactaaaatt
     3301 gacttgaaat atggaaatac acaatttggg tttgagaaat atagcgatgc tttagataag
     3361 tacagagaac ttaaagaaga atttaagtat gttccatgta ttataaaact tcattcatat
     3421 gtagcgcacc aagatttata tgtgaaagaa attaaagctg atggttttga aaagaaatat
     3481 ttagatatta aaaatttaat aaaattgttt aaagaacata aacaaaatat atcagatgaa
     3541 ctaagtcttt gtgataagaa aaaggaagaa gtattacatg taatagaaga aatgaattta
     3601 gacacagctt ctttaaatga taagattgat accattaaag aattaaaaaa cactttagtt
     3661 aagagaaggt tggttaaata tgaactaaat aaatactttg ccttttgtaa tacattatat
     3721 gaaattgaag aagagttaaa aaaatatgaa tctaaatata aacaaaaaga aaatcaagga
     3781 aaaagcagat tcaaaaaaat aaaaaatagg tgattagatg gaagaatatc attttaactt
     3841 aataaaaaag ctttgtgctg aaagaaaaat acacaaaggc tggtttagaa atattgaaaa
     3901 atgtggttgt ggaggtaaat gtaataagaa aaattgtaag atgttagaaa aattataggg
     3961 aggtacatat ggaaaatcaa actgaattaa cagaaaaaga tatagagaaa actttaagtg
     4021 ctttaaatat tattaaatat atttgtataa tatctaaaca ttgcaaaaat tgtcctcttg
     4081 gatatatgaa aaatcttgat gaacataatt gtatgctata tgaattagta gacaagggtt
     4141 tacttccatg tgattggtct gtaaaaaaag ttacaagatt gttagatacg acagaagaat
     4201 aaaacaaagg agctggttaa gtggagttag atttcagaat ttggaatgga tttagaatga
     4261 tacatggaga agaattaaga ttactaacag aagagatgtt tataaaaagt ggatattctg
     4321 aacaatacta taacgatatt tgtataccaa gagtagaaga tttgaaaata atgcaataca
     4381 tagggctaaa agattgtttt gaaaaaaaga tatttcaata tgatattgtt aaatttatct
     4441 ttgatggtgt ttggaaagta tgtttagtaa atttatcttt gccaaaaagg agcaacagtt
     4501 acatttgaaa gcataggtgg aaaatatact tttaatttaa gtgaaacatt ttcaaaagat
     4561 aggaaattag aaatcatagg taatttttat gaaaataaag atgtaatcga aaaatataat
     4621 cttagtattg aataggagaa gaaaatggat gaaatgataa ttagggtgaa aatgggagaa
     4681 ccattaccaa cactaggaaa gataatcaaa tgtactacat gtttaactaa tgaagagaaa
     4741 tgttttaagg ttaataagat atgtaaatta agttggatta acgataggaa tagtgaaggg
     4801 ttaattgtag aatgcaaagg taaatatatt aaatattaat aagaataatg aagttaaaat
     4861 caagatttta atagaaaagg agttattaaa aatgaagaaa aatccaataa gaattaaaaa
     4921 atgtgactgt ggtggagatg tatatgaact taatacacaa ttaggtacta tgtatcaatg
     4981 taaaagctgt ggcgagatgt atagatagtg ctaaaacatt agttaaggaa tatggggtga
     5041 agtaaatgag taaaaaagat aagctatctg tttttatcag tttaatatta agacataaac
     5101 cagaaattat tggaattaaa ctagatgact atggatatgc agatgttaat gaattgattg
     5161 aaaaaataaa taatactggt agaaatatta atatagaaat ccttgagcaa atagttaaag
     5221 aagataataa gcaaaggtac agttttaatg atgatagaag caagattaga gcaaatcaag
     5281 gacattcaat taatgttaat gtggaattga gggaattaga accaccaagg tttctctatc
     5341 atgggacagc gactagattt ttagataata ttaaaaagga aggtattgtt agcaaaagta
     5401 gattatatgt acatttatct aacgacatag atactgctgt tcaggttggg aaaaggcatg
     5461 gtgtacctgt agttttaaaa attaatacag gaaaaatgta tgaaaatggt tataaatttt
     5521 atttatctga aaacaatatt tggttatgta agtatatacc atttgaatat gtagagaaaa
     5581 taagtagagt agttaaataa aattatagaa aataggaggt tattgaatgg atttcaattt
     5641 tagaaagatt tgtgaaaaag atgagcttac tgatgatttt attataaaaa tttgccagtt
     5701 gtcacatgag gagtttggag gcactattta tgtaccatgt aatattcatc catgtgggtg
     5761 ggatgagttg gaagttccga atccaaagga tttagattca gataaattaa tggaagaatt
     5821 tctattagaa tataaatttg aatgtgaaaa tatttcaagc aatatttatg atagtgacca
     5881 tccatttata ggcaaaacat tgagagagat tcttatgata gtggattgta gattcataat
     5941 tggtgttata taaggaggat attaaatatg gaaaatatag aattaacacc tagagattat
     6001 tttgaggata taaaaaataa aaagcaaaaa aacacaaatg aaaatcttga tatagtatat
     6061 gaaaattctt taattctttt aaacaaatat attataactg gtcaaaaaaa gggtgctaag
     6121 aaattaatgt ttttacttga atgtattgaa aaagaaagaa gtgtaataga gctgggaata
     6181 gatacatttg tgtataaaga agatatagaa aaatatattg atgatatatc taaaaataca
     6241 gtaaaaataa tagaacttga aaattatgaa agagaaatac cagatgaaat agtagaaata
     6301 atagaaaaaa caaaagatat atttgataaa atgtatgttt tatttactga ttatacaggt
     6361 aaaattacaa agaagataag caaagagagg agagataaag acccaatatt atttggagtt
     6421 ttttcagatg agaaaagcag agctattgta gatagatttt attatcttgg agattgggaa
     6481 gatgagtatt gtgatttaac attagaaaaa atgataaatg attataagaa aaaaggaaaa
     6541 caagacatat caaagcatat aattacacct aaatcattag atgaattaaa aaaagaatta
     6601 gattctttag aaaaaaataa tgatacatac aagcagatta tcaatataaa gccattgcct
     6661 acgccaagta actctagtgt agaaccacct aaatcaaata tatttaaaaa aataaaaaat
     6721 agagtattta aaaatgaaaa ataatgttga tttaacagaa aatagggatt ttagagatgt
     6781 aagaggaaaa ggtcttagta ttttaggtat tgaagagaca ccaaatagaa tgttgaatgg
     6841 ttttagtgat tttggaatta gacatcttta tggtaaaaat ttagaatttg aatttgataa
     6901 aaatgaaata tttttaactg ggaatgcgaa tgacagagaa gaaaaaaagt tctttatgct
     6961 ttgtgaagaa aataaggttt gtatcagatg tggagatgaa ttaaaagagg gatgggataa
     7021 agtgagcata gaattgtgta ataaatgcta tgaaatgttg gatgttgagt ataaaaataa
     7081 aaagataatt agagaattat tttttagaga tagagatgta aatatagagt taatttaaga
     7141 gatatataag aaaaataatt agaggaggaa tttatatgaa agatgtttgg ttatatagtt
     7201 gggatgatga atgttttaga agtgatgaat acgaaagtaa agaagaagct gttgaggcag
     7261 ctaaggaaga acttaagagg ctcggagaga ttaaaagatg ggcttatatt ggaaaaaaag
     7321 aagaagttga tattcccgac atagatgcag aggatgcttt agagtttgtt caagatagaa
     7381 ttgataatga atttggaggg tatggagaag attggtttga aaatatacgt gctgaagata
     7441 tcgttatact agagaataga ataagcaaag tattcaaaaa gtggatagat gaatttggat
     7501 ataaaccata ttggtttgtt gttagagata cggaagaaat agaactaaaa aatgctgtca
     7561 attaaaacta gtattttcag tgatgacaac agtatgacaa gaataatgag tatataaagg
     7621 agtgatgttt attaaaggtt ttagatgtat attcagacat aaattttcaa ggataaaaga
     7681 ttgtcaatta attacaacaa ggagtcagca atatacctgt aaaatttgga tgtgtaaata
     7741 ttgtggacaa attgtagctg gttcaactcc tgtttatcat cctaatatga aagtcaatga
     7801 gtattgtata aagtgtgagt tttcggcatg tgcaaataat tatgagggat attgtaactc
     7861 taaagataaa gaaattgaat ttaagtctat ttgcttagaa gataataaag agattttgca
     7921 gtgtaaaaat cttgatttag aataaagttt aaattaaata ggaggatttt aaaatatgaa
     7981 aataaatgta tatgttaaaa ctaatagagt tggttcaggt tgtgaactgg agcttatgcg
     8041 agacctattg aaatgttttt atctaaagta gataaagaaa aatatcctag tgttattcaa
     8101 gaatataggt ttgaagaagt gtaaataagt aatgatacat agtttattaa acaaggaggg
     8161 tgtgattttg gacattaaat caaggttaga atcaaaagaa tataattttc ttagagaaga
     8221 agaaagatta aaaaatagaa tgatacttct tacaactgga ggaagttttg catatggaac
     8281 taatatagat actcctgagc atatatctga tttagacata agagggatat atttaaattc
     8341 agcacaagaa atattaacta tgaattgtat aaataagcca tatgaaaata aacaaacaga
     8401 tacagttata tatccattaa aacaaataat aacattatta ttaaatgtaa atccaaatgt
     8461 aatagaaata ttaggtacga gaaaagaaga cttatttgtt tgtactgaag aagggaagct
     8521 tttaaaagat aatgtagatt tatttttaac taaaagagct gctaatagtt ttggtggata
     8581 tgcaatatct caactaagaa gattacaaaa tgctttagca agagataact ataaacaaaa
     8641 agaaaaagaa gaacacataa tgaacagtgt aaaaaggcaa atgaaaacat ttgaagatag
     8701 atataaaatc gtatccgagg aagaaataaa attgtatatt gaagaatcta aaagaacaga
     8761 tttagaagaa gaaattttta tagatgttaa tctgaaaaag tatccactca gagatttaaa
     8821 aggtatgtgg agtgaaatga ataatatact aaaagattat tctaaattaa atcatagaaa
     8881 taataaaaaa gatgaattac atcttaataa acatgctatg catttaatta gattgttttt
     8941 aatgggcata gaaatactag aaggaaaagg gattaataca tatcgagaga atgataagga
     9001 gtttttatta gatataagaa agggaaaata tacctataat gaaatatttg agatggtaaa
     9061 tgaattagat aaaaacttta agtatgcaga aaaaaattct tgtctaccta attctccaga
     9121 tatgaaaaaa gtaaatgaac tggtcatgta tatgactaat agagtattaa acagatagat
     9181 aatcaaagag gtataaacat gagcgtaatc aataaagaca ttcattttta tgaagaaaaa
     9241 atatctattc ctaatgagct atggatttat tttaataagt ggttggaaac tagcaaaatc
     9301 aaataaggag gtattagttt gaatataaat aaattaaaaa aaataaaaga aagaaaaata
     9361 aaaagatgga atatagaaaa aattactaat aggataaatg accatatgaa tattcaggag
     9421 gatatagaaa aatgggataa atttgaaaca ccaattatta gtaaggaggt agatttttat
     9481 ttattgaaaa taggatttca atatggaagg aataatggga ggaatgtctt ggtaggaatt
     9541 aaggatatgc gtcatatatc aataagtaaa gttaattcta acttattatc aatagatata
     9601 tataaattta ttaaacaaga agatggcaaa tttagtgaat ttaaagcaca tgaatatata
     9661 gaaaagtaat taaaacaaat gttttaatgt gattttacct agtaggtaca aggaggtttt
     9721 taatgaaaga gcttttagaa gtaaaaaaaa tatttgatag tttagcatct acatctagta
     9781 gaaaagaaaa agagagaata ttagaaaaat ataaaaataa tagaatgttt gttgaatgtt
     9841 tacaatttct gctagattca aatattctca ctggaatatc taagaataaa atatgtaaga
     9901 atctaaataa tactagtcat aatgaattag agaacatata tgatatgctt gactatttaa
     9961 ttaagaataa tactggcaga aatatagatg taaaaacaat acaagtattt gccagtaaag
    10021 atgaaaaatt aaaagatttt atttttaact tagctacaaa atcaattaaa ttaggaataa
    10081 catataagac tgtagacaaa ataatgccag gtttaattat ctaataaatt ttgtgttttt
    10141 tacaaaaaac tattgagaac atagaggtta tatgatataa taaaaatata ttgagaacag
    10201 agaagttaaa aaggggggca atgagatgac tgacaaaata ataatagcaa gtgtagatgg
    10261 agaggaaatg atatttagaa ctgaaatgaa aaagttagaa aaaggagata gtcttatagt
    10321 ttctgtaaat ggtgtaaaaa gattagctct attcattagg tatataagta cttgtgaaga
    10381 atatatagaa gatatagcat ttcaaaaagt agatgatata ttatatgaaa acacaattgc
    10441 caaagaaaaa gaaagagtta aaaaggtatt taaagataag tgtatgaaaa attataaacc
    10501 tattgttaat acagaaatag actcttattt gagggaatgt ggtttcaatt gtgatggtag
    10561 acaatttttc aaaagtatag acaatacaca aattttaatt agcagggatt gtgataaaga
    10621 tgttttgact gtatcgaaag gtttgacaat aactaaaaca gatataacaa ataaaaattt
    10681 aattacagtt attaaaataa taaataatag tctattaata gttaggtaat agaaataaat
    10741 attgaacttt taataagaaa aaagatttta aataaaagga gtgatttaat gaagaatgtt
    10801 gttaaaagag atgggaggat agttcccttt gatgaaaaga aaataataaa tgcaatagaa
    10861 aaaagctttt tatcattaag gaacaaagga aaaagtgaag tatttgatag agaattagca
    10921 acactaatag ctaaaaaaat aaaaaataaa ccatgtgaaa aaatgcatat agaagatata
    10981 caagatataa tcgaatttag cttaatggct agtaaaaaga aagatgtcgc taaagaatat
    11041 ataacataca gagagaaaag aaatatagca agaggaagaa aaacttataa tgattatatg
    11101 tctattgtaa atactgaaag taatgatata actaaagaaa atgcaaatat gaacgcagat
    11161 actccagcag gtatgatgat gaagtttgca agtgaaacta ctaaaacttt tactgaccat
    11221 gtacttttat ctgaagaagc aaaagaagca atgatggata attatataca tatacatgat
    11281 aaagactatt atcctacgaa atcattgact tgtatccaac atcctttaga taaaattttt
    11341 aataatggtt ttagagcggg acatggctct agtagaccta ctaaaagaat agagacttca
    11401 agtatattag gatgtattag tttagagact gtacaaaatg aaatgcatgg aggacaggcg
    11461 ataccagctt ttgattttta taatgctcag agtgtaagaa aaacatttat agaagaaata
    11521 aaaaatatag aaataaagga tgatattgat ttatctcatt tatacaatat agaaatagag
    11581 gattatataa ttaaggactt aaaagaatta gacagcgaaa aaagaaatgt tcaacatgct
    11641 ataaacagaa ctgtatggag agttcatcaa tcaatggagt catttataca taatatgaac
    11701 acaattcatt caaggggtgg aaatcaggtc gtgtttagtt ctataaatta tggtacagat
    11761 acttcagcag aaggtagatg tataataaga gaaatactaa aatctactta tgaaggagtc
    11821 ggaaatggag aaacaccaat tttcccaatt caaatctgga aagtgaaaga aggtgtgaac
    11881 tataatccta ctgatagaaa ttatgactta tatgaattag catgtaaagt atcagctaaa
    11941 agatttttcc ctaatttttt aaatttagat gcaaccttca ataagcatgt aaaatggaaa
    12001 atgtgcgacc caaacagata tatttatgaa gtagcaacaa tggggtgtcg tacaagggta
    12061 tttgaagatt tgcatggaga aaaaacaagt ataggaagag gaaatctatc ttttacgact
    12121 cttaatttag taaaaatggc attagaggct aaagaaaaat atccaaataa tgaagctaat
    12181 agagttgcag agtttatttt tcttcttgat aaatattcag atgtagtagc aagacaatta
    12241 tatgatagat acttatttca atgtactgct aaagcaaaac agtttccact attaatgagt
    12301 gggttatgga aaaactcaga aaatttaaat ccaaatgatg atgtaagcga agttttaaag
    12361 catggaacac ttggggtagg atttataggt ctagcagaat gtttaatagc tcttataggt
    12421 gaacatcatg gtgaaagcga aaaggcacaa aaattaggtt tagaaatcat atcacttcta
    12481 aagtctaaag tagaaaaata taaaaagaaa tataacctca attattctgt attaggcaca
    12541 cccgcagaag gtctttctgg aaaatttact caaaaagata aaaaaatatt tggagaaata
    12601 aaaaatatta cagacaaaga ttattatact aatagttctc atgttccagt ttggtataac
    12661 tgcaatgttg aacataaagc gaaaatagaa gcaccttacc atgaattaga aggtggtggc
    12721 cacatatttt atgttgaatt agatggggat gcaacacata atcctaaagc tataatgaaa
    12781 gttgtggatt taatgaaaaa gtacaatatg ggatatggtt ctgtaaatca tacaagaagt
    12841 agatgtatga attgtggttt tgaaaacgca gattcagagt taaaagaatg tcctaaatgt
    12901 ggcagcaata gtataaatat tatccaaaga attacaggtt atttagttgg aactacggat
    12961 agctggaata atgcaaaatt agctgagtta aaggatagag tcacacatgg ataaagacta
    13021 tatttatatt ttagatataa aacatgacac aattgttgat ggagaagggt ttagaacatc
    13081 tatttattgt tctggatgta atcatatgtg tagaggctgc cacaatcctc aatcttggga
    13141 tataaaaaat ggaactttaa ctaaagtatt agatatttat aaggaaatca tttcaaataa
    13201 attttcagat gtaacatttt caggaggaga cccacttcta caactgcaag gatttgttca
    13261 tttagctaaa ttaattaaaa agaatacaaa caaaacaata tggtgttata caggttttaa
    13321 gtttgaggaa ctcataacag acaatgaaaa gctagaattg ttaaaattaa tagatgttct
    13381 tgtggatgga aaatttgaag aagataaaaa atctttagag ttgattttta aaggttcgtc
    13441 aaatcaaagg ataattgatg ttcaaaaaac attatatgaa aaacaaatag tattatatga
    13501 ataagagagg cttagccctc tctttggagg taagtgttat gaccaaagaa gaatttatta
    13561 ctaaatttct taaagaacag cagagaagaa taaaggtttt atattatgaa gttgaaaatt
    13621 tcaagatgga tagaaggttt aatgagagga aaaagtctag gagacattaa aatgtttctt
    13681 ttaatataag gaggaagtag ctatatgtat tttgaaacgg acattataaa aaataagaat
    13741 actgtaaata aaataataaa atattgtggt ggaaatactg aaaaattgaa agattggcta
    13801 aaagaaatag aaaataaaag tatcagtagt ttagaagaat taaaagaata tccattaaaa
    13861 agaaattata aatttgtatt ttccaaagaa gaagctccta atttagataa gcttataaaa
    13921 atgaaactaa gagataattg tttagaaaaa tgtgagattt gtagtttaga aaatctctta
    13981 agtatgctaa atgatgagat tttagattta aataaagaag atatctctta tgaagataat
    14041 attgaatgga ttgaaaattg taaagaagaa ctaattaaat taaaggtagg aagtatttgc
    14101 atagattggt agaaaaataa aggagtgata ttgtgagatt ttggtgtgag aattgtaata
    14161 aatacttcaa tgcggaagaa acattacagg aatataatta ttttttaaat gaagagatac
    14221 ttatatgccc tagttgcaaa aaagatttaa tacctatagc tagggaaaca gaattatcat
    14281 taggttttga tagtgataca aaccaattaa cttatgttga atatgattct agtgattata
    14341 gtttacttag aaaggtaaat gcagatgttg aagatgtagt aaaacctata atacattaca
    14401 taagaaattt aaataaaaat agtttagatt taaatggaat tacaataaca atgaatggta
    14461 atagagaagg aaaaagactt gatggattaa attatgaaga aggagtggtt atggataatt
    14521 tgataaatgc atggaatgga ttttgcaaat taaaaagaca gcatccttct gagttaggag
    14581 attttcaaaa tgctatacat caagctcaac aagtattagg gttaagagtt ttaagaaatg
    14641 attatccaaa aggttggatt aaaaaataaa tataataagg agagaaacca tgtctacaaa
    14701 aactatatta aaaaatatag atataaaaga tgataaagca gcagaaagac taatatcagc
    14761 tttggaagag tcggaaaata aacaagattc agatttagat attaaaaaga tatttgaatt
    14821 aatagacaat tctatttcct cttatattat atatggaact ccaaaagcat taagtaaatt
    14881 tgaaagtatc ttaaatatct ttaaaataga caaagcaaat tctcaacatg tccaaaaatt
    14941 tataaaagta ccagcaatag atttaggtaa atactgtgat tataaaaata ataaagccct
    15001 ttttgatgat aatacagtgt acattattcc aaaagataca aaattaaaaa tatataaaga
    15061 cgaatataag tatgttgttt tgaaatagat gatgatatat ataaaaagga ggttttaatg
    15121 accaaatata aaatagaaga tagaactaat cttataattc atcaatctaa aaaacataat
    15181 attaccttta ctttaattct agaaaatgaa gataaaagag aaatgcttaa aaaagtttta
    15241 agatgcttaa agaaggaacg tattgtgttt ctattgaagg taaattaagc agtgaggaag
    15301 tagaattagg aaatgttaca ataacaatgt ttgtagatgg aaatattatt ttaaaaatgg
    15361 atgtgttgaa atatctggtg agtataaagt tgaatagata aataaaatta gaggggagaa
    15421 tgaatatgga atttaataat tttaaaaaac aagttcaaga gagatttaat aaaatgacaa
    15481 ctggaatctc ttatatatat gaagtagatt tagataaaga taggctgtgg aatttatatt
    15541 tagatagttt tccagaaggt acaaacgaaa tatttagaga gagaagagaa tatgattgct
    15601 cttgttgtag gcaatttatt aaaaatatag gaaatgtagt aactataaag gataataaaa
    15661 tatcatcaat ctgggacata gacacagatt gtcctacatt taaacctgtt gtaaaagcac
    15721 tatctgaata tgttaaatct cataatataa atgatatata tgtaagtaaa tttaagaaaa
    15781 taggaactga taaaaatttt gaaaacacat ctgaaaaagt atatgaatgg aatcatttat
    15841 atatagaatt acctgataga tttgtggatg aaactaataa atctgtagga gatattaaag
    15901 gaagaattag agatgttaag aatgtattta aacgttcatt ggatgaaata agcgaagata
    15961 gtgtatctat agtgctagac ttaataaatc aaaagtctct ctataagggg gaggaatggc
    16021 aaaacccatt gatagaattt ctaaaatata aaagaaatta tattaaatta caaactgaaa
    16081 ctgataagca aaatttcgca tgggaacaat ctgtaaaagc tggagttgtt ataggtaaaa
    16141 taagaaatca tagtattggt actttattac ttgatatatc agaaggagtt gaattagata
    16201 aagctgtaag tagatatgaa aaaatagtag ctccatctaa ctataaaaga ccaaaggcga
    16261 tatatactaa aaaaatgtta gacgaagcaa agaaaactat aactgaacta ggatatatgg
    16321 attcacttca aagaagacac gctacaatag atgatattac tgtaaataac atattatttg
    16381 ttaataagga ttcagcaaaa caaatgggag aatttgatat atttgatgaa atgtctaaag
    16441 gcataggtat agattctaag aagttttcaa aagtagaaga attaaatata gaagagttta
    16501 ttcaaaatgt tctacccaca tcaaaagaag ttgaactgtt attagaaaat agacatatag
    16561 acaattttgt atcattaata gcaccagtta ataaggaggc caaatcaatg ttgaaatggg
    16621 ataataactt tggatgggct tacgctggaa atatgacaga tagtagcatg aaggagaatg
    16681 ttaaaaatgc aggtggaaaa gtagatggcg tattaagatt tagtattcaa tggaatgata
    16741 ccgaattcga taacaacgat ttagatgctc attgtataga accaaatgga aatcatatat
    16801 attttggtaa taaaacgaat tactctacaa ctggtatgtt agatatagat ataatgcatc
    16861 caaaaagagg taatgttgca gttgaaaata taacttggtc tgatataaac aaaatgagat
    16921 taggtacata taagttttat gtaaataatt actcgaagag aagtggaata tctggattta
    16981 gagctgaaat agagtttaat ggtgaaattt attcatttga ttataacaaa cctttaagag
    17041 atggagaaaa tgtaatggtt gctgaagtaa cgttagatat aggtaataga tttaaaatta
    17101 agccattgtt accttctaat gtttcaaata aagaaatatg gggattaact acaaatcaat
    17161 ttatacctgt aagtgtaata atgaactctc ctaattattg ggatgggcaa caaggaatag
    17221 gaaataaaca ttatttcttt atgttgaaag attgtataaa tgaagaaaca gtaaatggtt
    17281 tttataatga atatctaaaa aatgatttaa atgtacatag aaaagttttt gaagcactag
    17341 gaagtaaaat gtatgttcct tttagtgaaa atcaaatgag tggattaggg tttagctcta
    17401 ccagaagaaa tgaagtaatt gtaaaagtta gaggaagtta tgaaagaata ttaaaaataa
    17461 aattctaaag agagagagag gtaaataata gtatggaaaa tgtatttgag atggcaacaa
    17521 gaaataaatt tagatttgaa tttagaggat taatttcagt tgaggattta tgggatttaa
    17581 aattggagca gttagacgaa gtatataaaa ccttaacaac agatatgaaa aaagctaatg
    17641 aggagagctt attaaagata aaaacaaaag accaaaagga agtagaatta aaaataaaaa
    17701 tagttaaata tatagtagag ttaagacttg cagaaataga aaatagaaat aaagaaaaga
    17761 agaaaaaaga acaaaagcaa catatattag agctaataaa taagaaggaa aatcaagaat
    17821 tggaaaataa atcaatagaa gaattaaaat caatgattaa tagtttagag tagttctagt
    17881 aaaagatgct cttgtttaat agagcatctt tgaataaaaa acagattcta ataagaaagg
    17941 gtgttagaat gtggatagta aaatagaagt tggaagcata tggggattag aagatgggat
    18001 agcacaacct caatatgtag gaacaaaagt taaagtggtt tctatttcat atatagaaga
    18061 atttaataaa agacttataa cgtttatgga aattgaagaa aaagaagaag atacttggat
    18121 agatgcagat tgggaagatg tttttttaaa tgatttcttt aaattagact aattaaaaca
    18181 acagttttaa gatgaataaa atttaaaagg aggattaata aatatggata acaaagtatt
    18241 gatacagtta acgaatggag caattattaa ggagaaagat actaaatgta ttctaaaggc
    18301 aatgtagtaa aagtgtggtg ctatcagtgt gaaaaaatag aatatcaaga atttgtagga
    18361 tattttaatt gttattttga taattataaa ttaaagaaac gatggaaatg ctctaagtgt
    18421 ggtgttatca ataattttta acaatttaat taatggagga gtgagttatg aaaataaaga
    18481 aaataatttt aggattagat aacttagaga atgtagagtt gggtattgga gatgtagggg
    18541 ctttttattt agataatata catgaaatta ttggaggatt tactttgagt tatatagata
    18601 aatgtaaaca ggcagattat ttacatatac aaatacataa acgtgttgat gaaattcaaa
    18661 gattgcatga ttataaggat attacaaatg taaagataga atacgaagac ggaagtgtgg
    18721 aggatatata tgtaccttat aaacaagaag gggatttctt gggtttagat aatctttatc
    18781 aaacttcttt tattagtaaa caaggttatt tacatatagt tatctctaag gataaaacag
    18841 tagaagaagt cttcaatgaa gaatttgagg atgatgaagg ggtagaattt agttttaata
    18901 tgtatggaat ataaaactta aaatggaagg agatgagcaa atataattaa ctcttttaag
    18961 aaattaataa agtcaaaata tataaaaaat aacgaatcaa taaaaccatt agaaagaatt
    19021 tttgaatatg aaataagaat caaagaaaaa agaacatatt ttaatttaga attattgatt
    19081 tatgaaaaaa gaattaagat aattaaaaaa atatttttaa aagatatact atgccattat
    19141 cgaagagata tagaacaact cgtcatttat gacctggata aatatcttaa tatgacagat
    19201 aaagaaatgg aggaatatat tataaattta ataaaagata aaataaaaga agagtatggg
    19261 gagaatatac ttcctgaaat actaatggaa aaatttataa aactcgaaag aaaaggttcg
    19321 gtgactgtag acgtacggga tttaatagag ggataataaa taacaagtta aaatacaggt
    19381 tttaaaagga gaataataaa tggaagttca agggaaatat aacaaagcaa aaatatttac
    19441 agataatgta gatagtacaa caataagtca aataataaat ttatgtaatc aaaaggaatt
    19501 taaagaatct agtataagaa ttatgcctga ttgtcatgct ggtaaaggat gtacaatagg
    19561 aacgacaatg actataagta ataaagtagt gccaaatctg gtaggggtgg atataggttg
    19621 tggaatggct actattccat tagaaaaata tattaataat ataaattata caaaattaga
    19681 taagattata agagattata ttcctcatgg atttaaaata cataataagt caagagaaaa
    19741 actattaaag aatttattta atatagacat aaataatctt agatgcgagg tgaataaaaa
    19801 tagagcatat atgtcgttag gaactttagg tggaggcaat cactttatag aagttgataa
    19861 aagtgaaaaa ggtcaaatgt atcttactgt tcatacaggt tcaagaaatt taggaaaaca
    19921 aatagctgat tattaccaag acaaagctat aaaatattgt attgaaaaat ataaaaagtc
    19981 atatgatgat gcaaaagagt ttttaatatc tactctaaaa aaagacaata aagaacattt
    20041 gataaataaa tatttgaaag accttagtga tagaaaattg aataagccac atgatgattt
    20101 atgttattta gaaaatgatt taatggaaga ttatttacat gatatgagta tagctcaaaa
    20161 atacgctgct gcaaatagaa tggttataat tgcggatata ttgtttaaat ataacagcct
    20221 agatgtaaat tatgaccatt ttataagaga tattaaatgt gatgttatag aatgtattca
    20281 taactatata gatatggata gtaaaatact tagaaaagga gcaatttctg caaataaaga
    20341 tgaaacagta ataattccag taaacatgag agatgggatt atattaggaa aaggtaaagg
    20401 taattctgag tggaattatt cagcaccaca tggagcagga agaattttat caagaggaaa
    20461 agctaaagaa aaaatatctt tagatgaatt tgaagaatct atgaaagaaa tatttacaac
    20521 ttgtgtaggt caatcaacac tagatgaagc accacaagca tacaaaccta tagaggacat
    20581 attaaacaat ataggagata ctattgagat aatggaggtg ttaaaaccag tttataattt
    20641 taaaagtaat taaaatgaaa aatttaatag atgaatttag gaggaaatac taatgggaat
    20701 gtatacagaa tttgtatgtg cgattgaatt aaaaaaggat acgccaaaag aagttattaa
    20761 tatattaaat aacatgatag aaggagaaga tagatatgat attacaccac ctcacccatt
    20821 ctttgaatgt cgtagatgga gatggttatt tataatggat agttattctt tctcaggtag
    20881 gagcaatacg atgtttgagt atgacgatat agcaaaaaca tattatttaa ctattagaag
    20941 taacttaaaa aattatgatg atgaaataaa taaattttta aattggataa aaccatatat
    21001 acaaatacat tgtgaagacg atgaattttt aggatatagt cgatatgaag aatgtcgtaa
    21061 tccagaatta atttattata atgaaataca ataaaatctt aattttaaat ggaggtacat
    21121 atgaaatttg aagataacat gattaaatct tttagagagg ttagagttgg agacattata
    21181 cttgcaggta ataacaagta tttaataata tcaatattaa attattttgg atatgtgaac
    21241 ttagatactg gtgattgtag ttctggcttt agatcaattg aagaattaat gaaacaattc
    21301 gataaacaaa cacgatttat tcaaaatgat agatttaaat taatactaga ttaatataac
    21361 ataaaatttt atttttataa gaaaggtaaa ttgaatatgt gtaagttttg tgaaagtata
    21421 attgataata caaaagatat acttttaacc tcaagaagta gattattaga agacaataca
    21481 tgtgaaatta tagcagaaga taattgtaat aattgttatg atggatgtta tgaatatttc
    21541 aaactaaacg gatataaaat caggggaaat acatatgtta atgtcgacta ctacaaaaaa
    21601 attagaaaaa taataattgc tccatgttca gaatcagtac atataaacta ttgtccatat
    21661 tgtggtaaac aagtttctaa agatataaaa gattttaatt gtattcctga gcatatagta
    21721 gatgttagat ataaaaatgg tgatgtatat gattatgata tggaaaagtc tgtacaagaa
    21781 gtcatggata atgcaaataa taattaaata aaatgatact tttaataggg gataaatatg
    21841 aatctttggt ctaaaattga agaaccagtg atgagagcag taagtggaat agtatttaat
    21901 atatggggaa atgtttgttt ttccatatat tacatagaag aaaataaaca aatagagtta
    21961 gaaatatatg atagacctta tagatatgaa ggtaaaaaaa atagttgttg agaagttatt
    22021 tgattacaag ttaataaatg cagattataa agaaatacat gatattgttt ctatgataga
    22081 aagattttta ctagaaaatt attaaaagga gatatttaaa atgaataaag gaattaaaaa
    22141 ctatataaca gcagtgaatt acttacttga taatttagag tatatgatgg aggatgacga
    22201 atttaataat gaaaagttac agaaaagaat tgatgtaatt agaaaatttg aaagagaatt
    22261 ataagatggg aaaaataaaa aatggagaaa tagatactga agattatagg ctttttaaaa
    22321 acattcaaaa agaaaaagat agagtatata gtaagtacat taatgaagaa tacataatgg
    22381 aagaattcat aaactactta agattggaag ataaagatta tactacagaa atattagaaa
    22441 gatatgcacg atatattgat gaaaatttag acaatcgttg tccatattgt gtatataata
    22501 tttgtagtga acatgataat tgttttgatg gaatacttga aaatttaaaa gaaaaaatta
    22561 aaataaaata aatattttaa caggagaaaa ataattatga aattaataaa tggggataga
    22621 tgtagtggaa aaactactac attaataaag tatgcttatg aaaataatgc acttatatta
    22681 tgttattcgt gtggaaattt gaactatata attgaaaaag caaaggaatt gaaactagat
    22741 ataattatac caagagtatt taaaacttat atagagtcta atcataaaat agaatatatg
    22801 aaaactataa atggaaatat tatcaacgat acttctaata taagattagt tatagatgaa
    22861 atagattgtt gtttggaatc tattattaga gaaggtatag attgcgtcac tggaacaatt
    22921 caaatagaaa atttaaataa tcatatctga taaatttgtt tattacatat caaaaaattc
    22981 taagtgttct tatttacaat tcaattaaaa ctaaaggagt gaaattatat tgaaaaaaat
    23041 tttattaata ggtgcattag gaacaatgtt aataggaatg tctggatgta caggaataca
    23101 aagctcaatg aaagatgctg aaagtgatgt atcaggtctt aatagagttg taaatgtata
    23161 ttcagataat ggcgaggtat taaaaacata tacaagtaaa agtatgagag tcaaagatgg
    23221 agatggtggc actataacac tagattttga tggtaagagg gtgattatat gtaatgctca
    23281 tgtagtaata gaagaaaatc aatctaacaa ataaaggaga actaaatgtt taaagtctta
    23341 aaaacaattc ttataataat ttgggtagta gatattttaa acttcccaca atttcaattc
    23401 ttagatacaa catatccaat caatacctta gcatggttgc taatctggat tcttatacca
    23461 tcaagtagta tatatattga taaaaaagaa taaagaaggg agtattagtt tgattttaaa
    23521 tattggtgca atttttaatt tatggtgtat gttagctaat tatttatggg gaagtactag
    23581 tggaatgata cttaatggat tttgtcttat agcattacta ttattggata ataacttaga
    23641 ttaaaagtat tattttaagg agattaaaag atgaaaataa caatatcaat tgaagaagca
    23701 atggatatta ttaaacagca ctatgagaat aaaggaattg aaatcaaaga cttaagtttt
    23761 aatttagaaa aaactggaat ggttagaaat attaaattta agggagaaat aatacttaag
    23821 taggagatat ttatagttgc atcagtaaat aaaatattag acaaatagag gtgatactta
    23881 taagtagtac aaatagaagt aatgctaggg attttcatgt aagtgattat tatagaactc
    23941 caattactaa aatcaaagag tttttaactg agttcagtag atatgaaaat atactcaatc
    24001 caaatataaa aatattagac ccatgtgcag gtggagataa tacaaatcca atgagttatc
    24061 caactgcaat tcaagaattt ttaaatcaag atatttcaat agatacagta gatataagag
    24121 atgattcaag ggcaaatata aagcaagatt atttaaagtt tcagcccaaa gaaaaatacg
    24181 atgtaattat tactaatcct ccctttaatt taagcttgga tataattaat aaagctttaa
    24241 atgatgtaag agaaggtggt ttcgttatta tgctgctaag attaaattat cttggaggaa
    24301 aagtgagaca aaggctgtgg ggaaataata tgcctaaata catatttgtt cacaatagaa
    24361 gaatgagttt tacagatgat ggaaagacag atagcataga atatgctcat tttgtatggc
    24421 agaagggata taatccaaaa ttttctcagt taaaagtatt aataagtcaa tgaaatagag
    24481 gtgtaaagtg aaagttaaat acgttgtatt tgaatgggag attacatcaa aaaatgatgg
    24541 ccaaaaacat tttataaatt ttagagattt aattaaatta tatggagtgt cgtcaagaga
    24601 atgtataaga gcaaaggatt attacgagag agatggttta gaccttaaag atataaaatt
    24661 tctatatcca agggatgatg gaaaatataa actataaaat catggtttta aatgattata
    24721 aaatgtcagt tttaaagggg gctaatcata tatggatatt aagatagata tgtgtttatt
    24781 agagatggaa ataaatagaa gaagatgtga gtatgctgcc agaacaggaa gatatcctaa
    24841 ttctcttatt ttgtgggaag gttactatag ccttcttaat atccaatata tagaggataa
    24901 aacaagagga tatattacta aatttaatgg catggatgtt ataaaatgta atgatttttt
    24961 aaaaatagaa ttatttgaaa agaatattaa taattaaaat ttgtttttta agttgaggtg
    25021 atgagttgag atataataat tatcatagtc ataaaattta ttcaaatata aggtcgttag
    25081 atgtaattac taaaccacaa caatatatag atagagcaat aaaactaggt caaaatacat
    25141 attttacgac agaacatgga tatcaaggaa atgtatatga ggcaaaaact ttatgtgatg
    25201 aatataaatt aaaaatgatt attggagcag aattttacta tgtaaatgat ataaatgaga
    25261 aagatagagg aaattatcat ttaatagtaa tcgctaaaaa taatgatggt tacaagcaaa
    25321 taaataaagc tttaagttta gctaataaaa atggatatta ctataaacca agaatagatg
    25381 aaaaaatatt atttgagata tttaaaccag gggatgtagt tattactact gcatgtgtag
    25441 ctggaatatt aaaacttgag aatagagaag aattaatact aaaactaaag aattatttta
    25501 aaaataattt ctttcttgaa gtacaatctc atcctcataa aactcaagct ttgcacaata
    25561 aggatgtttt agaattaagc aacaaatata atatagatat aatacacgcc aatgacagtc
    25621 attatattta tccagaagaa tcaaaatata gaactaaatt tcttaaagct aaaggtataa
    25681 attatcctga agaagacggt tttattttag attatccaaa ttcagaggat atatttaaaa
    25741 gatatgaaaa acaaaatgta ttaacaaagc aagaagttga aagagcatta aaaaatactc
    25801 taatatttga tgagtgtgaa gaaatcacct taataaatga tgatataaaa ttaccatcaa
    25861 tatctaaaaa tccaaataaa gaactgaaag aaatattaaa taaagaatgg ttagagaaaa
    25921 gaaaaaatat acctaaaaat agatggaatg aatacttaga tgctataagg tatgaatttg
    25981 atattataga gaaaactcat atggaggatt actttattat agattataaa atagttcaaa
    26041 gagcaaaaca agaatataat ggtcttctta ctaaaacagg gagaggttca gcaccttcat
    26101 ttataataac taatctttta gggcttactg aaatagatag attaaatgct ccggtaccat
    26161 tattccctac tagatttatg agtgttgaaa gaatactatc agcaaaatct ttgccagata
    26221 tagatttaaa tgctgaagat gctgaaccat ttatacaagc aactagagat ttattaggag
    26281 aagaaaattg tgcatggatg ataagttata agccattaca agatgcttca gcttttaggc
    26341 tatggtgtaa agcaaatgat atgaaactat ctgagtatga tgaagtagct aaaaacttag
    26401 ataaatacac aaatgatgtt ttttggaaag atgtaataaa ggaatcaaag gtatttgtag
    26461 gagtagtaga aagtgtttct ttctcaccat gctctatgct tatatatgat agaccaattg
    26521 atgaagaagt aggattatta aaaactaagg atggggtatg ttgtaatata gatggatatt
    26581 attgtgataa gtataaatat ttaaaaaatg attatttaac agtgaaagta tggtcattaa
    26641 taagaaaaat ttgtgaatta gcaaatatta gtattcctac aattgaagaa ctaaacatct
    26701 tattagatgc taaaacctat gacatatata aggacaaatt aacttgcact ataaatcagg
    26761 tagattcaga ctatgctaca aacctggcat ctaaatacaa aatatctagt gtggcggaaa
    26821 caagtgcatt tgtagctagt atcagaccag gatttgcatc tttattagac aattttatta
    26881 acagagagtc atatacaact aacgttaaag aactagatat attattagaa gatagttatc
    26941 attatcttat gtatcaagaa tcaataatga aatatttaat atggttagga atagaggaat
    27001 ctgagagtta tgatattata aataagatta aaaaaaagag atttaaggaa aaagagttga
    27061 aagaattgca tatcaaacta aaaaataact ggattaaaaa agtcggaact gaaaataatt
    27121 ttgaagatac atggcaagtt gtaaatgatg ctgcctcata ttcctttaat gcttcacata
    27181 gtctttcata tgcatatgac agcttatact gtgcatacct taagtcacat tatccattgg
    27241 aatattatac tgtagcattc aatttatata atgaagatac agagagaaca agaaaattaa
    27301 cagatgaaat agagtatttt agtattaaat tagaaaatcc taaatttaga ttttcaaaat
    27361 ctgaatactt ctttaataga gacacaaata gtatttacaa gggcattgaa tctattaaat
    27421 ttttaaattc tgatatagga gagtatctat attctcttaa agataataaa tatgattcat
    27481 ttttagagct attaatagat ttgcaggggc atataaactc taaacaatta tctatactga
    27541 ttaagctaga tttctttgag gaatttggaa agtccaacaa acttttagag acttatgata
    27601 tctataactc aatctatagt aaaaaacaat ttaaaagaga taatttacca tgcaatataa
    27661 ataccatgag aaaatactct aataaagaaa cagaaaaaat atttaaagaa gttgatacaa
    27721 aagaattatg ttcttatctt gaaagtcaaa ttgaaaatac agatatacca accaatgaaa
    27781 aaatacaagc tcattttgag tttgtgggtt catgtaacat aaaagatagc aattcaaatc
    27841 caagatattg tttagtaata gatatagata ctaaatattc tcctaaagtc acattgtata
    27901 atataagttc agggaatata aagattttca aagtaagtaa acctattttt gaggataata
    27961 aaattgatgt ttatgattta gtttatttaa aatcaattaa agaaaaagct aaaagtaaaa
    28021 aagtagatgg aaaatggatt aggagcaata cagaaacaga atggtggata caagaatatt
    28081 ggaaaataga aaattaagaa tatgacctcg tatatgcgtt tctaagactt tacataagag
    28141 tctgcttgtg tttatacatt gatttcttaa aatggcatat acgaagttcg tgaaaggtag
    28201 tgatttcaat acttatagat gaacaaagat attatagctt attggaagaa aaaaaagacc
    28261 taagtatgag aatcgatgaa ttaaaagagg aaaatgcttt tttaaaacaa gaaaatgaag
    28321 agtatgaaga aattcttaat gagtgggaat gtgtaagaaa agaaattgta gcaacttgta
    28381 aaaaaaaaga aatattaggt gaaaaaccaa gtgaattaga aatggttctt caaaaaatag
    28441 aggatatata atgagtgtaa ataaaattta tgagaaaaaa gaatacatta ttatcaaatc
    28501 aaaatctgga tatatagttt ataatacaaa taaagagttt aaagaaggcc acactcactt
    28561 aaaaagttat aatgctgcta aaacagctat agatttagtt attaagagaa ggcttcctaa
    28621 aagttattct atttattttc ttacaagttt gatgagaata tctaatgatg tacaatatat
    28681 aaaacatata agcgatattg tatatataag aaataataaa ggactaaaag aaaaatatat
    28741 aaattctaaa aatattaaaa aaaactattg agaacataga aggtatatga tatactatga
    28801 atataacaag aacatagaag gtaaaaagga ggtagtgatt aatatttgaa atcttataaa
    28861 gaaaaacagt ttttagtatt tgaatttgaa aatgggaaga atgtaaagta taatttggca
    28921 acaggagaat acgttggtaa atctggaagg attgtaaaag atgtgtgtac tcaacttaga
    28981 ggatataatt tacatgaggt aataaattct tttgaagatg aaaattataa gtgtttttta
    29041 aattttgtag atgaaagagt aaatagaagt aagagtagca gagcttgggg aaggagggtt
    29101 gaaagaataa gaaatatagg aagtttttta aatgaaataa ataattattc tgagtatgaa
    29161 caattattct cagcaggatt aaaaaaaata atttatccga tacattgtga tattaagagt
    29221 atacctaaag gtcttatttc tatctgtagg aattatagta ttactttgag tgaccgattg
    29281 atacaagggt atagtaaaaa cccaaatttg tataataatt tattaagttt agaattgaat
    29341 tcaataagaa aaactaattt aatttcaatt cttgattctg gttatgatag aatgatattt
    29401 gatagattaa tagacatata taaatataaa cctcaatcac ttataaatta tatagataat
    29461 ctgatgacat atgaagcatt aaatgggctt gaaagcactc taaacgaatt atatgactat
    29521 gttgttatga tgagtgtaat aagtgataaa tatgaaaaat atccaagaca tttcttaaca
    29581 actcataaaa tagcatctcg aaattataat cgtcttaaaa ctcaatttga agaagaagta
    29641 tttaataaaa ggatagacaa gtcactagaa tattcatatc aagattataa atttatatac
    29701 cctaaaaata caaatgaaat taaagatgaa gcagtacaac aaaataattg tgtaagtagt
    29761 tatattcaaa atgttataga tggaaaatgt catatattat ttatgagaaa aaaggataat
    29821 ccaagtaaaa gtttagtaac aattgaagtt agaaatggca aaatagtcca agctaaaggt
    29881 aaatataata gagatgtcaa tgaaagagag caagaagcaa ttgataaata taataataga
    29941 atagaaagga tgaagaaaat atgttaatag aaagaactaa aataagatta atgaaagaaa
    30001 tacaaggttt tgagatgtta aaaataggag atatatttgt tattacatct gtgggaaata
    30061 atggagctat acattttaaa acagattatg gaataggttt tatgaactat agtgaatttg
    30121 aagcatattt tgaaattgtt caacaaaaga aaaaatatga atggggtgca tggtctataa
    30181 gaggagattt ctctggagca tatctatata gaacaaatgg gaaaaaagta gaagttagaa
    30241 aaggaaattt taaagcatca gctacttgcc atgattcaga tgagtttgaa ttaaataaag
    30301 gtgttgagtt atgtctggca agaattgaag ttaaaaaagc aaataaacag gtgaatttag
    30361 tattagataa aataaataat aaagaaattt aggaggttag aatgatttgt aaatttaaag
    30421 aaagatgtga aaataaaaat agagattgcg attattgttg ttataatcca aatgcatatt
    30481 tagaagatgc ttttgaatgg aatggagaag ggagaaagcc aacagacgaa gagttagatg
    30541 atgctcttga agaataaata agtatttaaa attatcattt taatataacc aggaggtatt
    30601 tatggaaggg ataataaata gacctgtatt tgatttaaat acatttttca ctggaagtgc
    30661 aataagttat ggttacagag gaaatagtaa acacaacgca ttaatatcta gtataacacc
    30721 atttcaaata gatgtggcct atatcagcgg aaataaagta gaaagtgata ttataagtct
    30781 tcatcatgtt ttagatggac aaatagaaat agagttatta aaattttaag tgaatttaaa
    30841 gaaaagaggt atatatgagg tggaaaaaca tattagtaac aggagcttta atagttggta
    30901 gtacaataca tgtatttgct aatacagaag atgttgttaa taataatata aataaagaac
    30961 ttatagcaga tacagcaagg aatattaatt taatgtataa aaaagaatgg gagcaatata
    31021 aaaatactaa agaatataaa ctagaaatta aaagaattaa tttagaaaaa gaattagaaa
    31081 taaaaataga aaaactaatt cctatgacat ggaaagtaag ttattacact tctttaaatt
    31141 gtgagaatag tagatatgga gctgttacag caacaggaga aaaattaaga tatggatttg
    31201 tagctaataa tcatcttaag tttggaacta aaatattagt tgatggaaat ttaaaggtag
    31261 ttaaagatag aggttctaat aaatattttg gaaactctaa tgcaatagat gtatttgtgc
    31321 caagaataaa aggggagagt gattataaat attataagag agtaaacaac atgggaagac
    31381 attataaaga aggatatatt atagttgaag gttaggagtt gataaattga aagtaaatgg
    31441 aataaaaata ccaggaatta ttgatgtatc aaatgtgtgt gtttatggaa ttaaagaaag
    31501 tataatttcg agtggttatc ctatgcagca acagattaaa gatatgagag aagtaaatat
    31561 ttcagaaaaa gatttacaac gagcatgtca attaggcaaa tcaaaacaag gttcaggcca
    31621 tgattgcttc tctaaaggaa ttattgttca aatggatttg caatggtcag aatatatgtg
    31681 gagacagtgg gatagatatc atcataatga ctatgtatct agtcaaagta aaatgcacag
    31741 attaaccaaa tttaatattg atgatatgtg taataagtat gttgttaaaa atacaataaa
    31801 tttcctgaat gaattgattt acgattataa taattttcag gaattaaaat taaccgcatc
    31861 tgatgaagtc actcttagaa atggagaaaa tattagagcc acaaaggaaa ctctttggaa
    31921 aattataata tcaaattgcc catcaggatt aatgttaact gcaagagtta cattaaatta
    31981 tctacaaata aaatctatga taaatcaaag agaaaatcat aaaatggaag aatggagaat
    32041 tctgtgtgac ttttttaaaa cactaccaaa atataaagaa ttaatgatgt aatagttttg
    32101 ttagcaatta aattttaaat ataaaggaga agtgggtatg gcagaaaaaa gaataaatta
    32161 taatcaagta tttgtatcag gagaagtttt agacatttta aatatagcta gaatgaatca
    32221 aggaactggc caagaagcta taagatttac cttaaaggtt gaaacagcac caaacgaatc
    32281 agtaaatgta gattacttta catcaatgtt taagtcagat ggttcaccaa atcaaatgtt
    32341 tttaggaata gagacaatag ctaatgaaat aaaaactaga gcagaagatg gaaaaggtga
    32401 tattgttaga tgtgtttgtt cattagacaa taacttatat tataaagatg gtgaaaagaa
    32461 agagcgattc caaatatcag gaactttttg caatagagaa aaatatgatg acaaaggtaa
    32521 tcctattgat gacaaaggcg aagtagtaaa gtcaataaaa gcgagtcagg tctggagagt
    32581 atatacatta attgaaaata tagaagaaaa agaagatgat ttaggtaagt atttagagat
    32641 taagggttta attaataaat atggtaagca aggttgtaat atgacattta gaatacataa
    32701 tgaagatatg atagaaggat ttaaaagttt atataaaata ggagatgttg gccttcttga
    32761 aggtacagta aaatctatgg ttgttagtaa atcagcagga tttggttcaa gaattaaaaa
    32821 aagtgctttt acatttttag aaatagaagg tggcgatgaa cctttgaaag atggagacaa
    32881 aatatttagt gataaaaatt atcctttcac agataagaat atagaggcaa tgagagaaaa
    32941 aataaaagaa aaagatgaaa aagaaaaaca aagagatgtt gataaaaatg gtaagacaat
    33001 tgagataagt gatgatgatg taccatttta aattataaat taaataatct attaaaaagg
    33061 agagatgtga atggaagtta aagaaaaaac aatgaatgat ggtttcaaga aagtggtaaa
    33121 tgaggaaata aagacgtata tatcaaattt atattcagat agaatagacg gattaaaaga
    33181 gagtgatagt caaattgaga taacaaaagc attaatagcc tttcatttag atatagaatc
    33241 aattccaaag gataatacaa acccattttt caggtctaaa tatgcaaacc ttgatacaat
    33301 tcttaaagca gtaaatccat tattagcaaa acatgattta atactaatgc aatcagcaga
    33361 agatgcaggt aatgataaag tatatataaa aactaaacta aagcattcat caggcgaata
    33421 catagagtca aattctgcac catttaaacc agctaaaact aatgatatac aagctagagg
    33481 agcattggaa acttatttga gaagatatgc agttcaatca gtattagcat tatcattcga
    33541 ggaagacgat gatggaaatt ctttaacaaa caaatcaaag ggtaaatcag aagaagaaaa
    33601 ttcaactatt aagaaaagta gagtgtaaat aaaattgcta cctcttaaat gaggtagcaa
    33661 taaatacata acataaggag gggaaatctt gaattgtaag tgtaggtttt gtaaaaagaa
    33721 attaaataca aatgatgctt ataaagtaga gcatattaca tctgggggaa ataagcagaa
    33781 tagatattat tgcaatgaac aggaatatag aaaagagcaa caggatatat atttctggaa
    33841 acaatgtcaa cttggaatag attatattat gggatataca gttataaata atcaaaaaaa
    33901 taagatgtta caagagataa taaaaggtgg ttatacgaga gaagaattat atgattgcat
    33961 cattgagaag aaagatgaag ttatagaatt acttaactat agaaaagata tagaagaaga
    34021 atatcctaaa ttatgttatg tgtttacaat attgaaagga agtattagag atataacaat
    34081 taaaaacaag caaatgaaat atgaaaaaga aaatgaaagg atatacaaag aagtagagaa
    34141 aagtgaagaa tattatgaaa taattgctcc taaaaaagta atttataata aaagacaaag
    34201 tttatttgac aaagtgaaag gagtgtatca agacgagtag ttatgaaaaa ataatagagg
    34261 ataggggtat tatagaaaca ttggttttag gcacaatgtt aaaatcatta actcttttta
    34321 gtgaatataa aattagcgaa agtgacttta taatagataa ggtaaaattc tttttctcgt
    34381 taggaagaat aatgtctaaa acacataacg agcttgatga aataagtgta gctaaatttg
    34441 tatcttcaaa taaactcaaa tctgaatatg aaagatatgg tggttggaat agtttatcat
    34501 ctgctatgga gtatggaaaa gaaactaata tagcagcata tattgatgat ttagctaaga
    34561 ataatttact aattgcttta gataaaagag gttttaatgt aataaaagaa atggagcata
    34621 atgggttaaa atttattcca tttgaaatat ttcaatctat gaaatgtaga gaagtggaag
    34681 aattttatga ggggttaata tcttcatgta gtgtaaattc tataaaaaat aatatgaaag
    34741 ttgaaaatct tttacttact aaagatgata gagaaaaatt aaagcaaaaa actgaagcag
    34801 gaacacctta taatattata tttgaataca ctgaaaaaga aataggattg agtaataatg
    34861 aagaatctaa gtatatatat agcttaccaa tattatcaaa taggactaat gggattggta
    34921 agggtggtgg tataaatata atagcagggt tttcaggcat aggaaaaact actttactat
    34981 tttttaatta catattagct atgatatata gaggagagaa gatagtcata tttgcgaatg
    35041 aacaaaaatc tcaatatttt aagagtatgt tagtatcgtt tatagcatat aacatattta
    35101 attatcatga tttagataga aataaaatag ataatggaga ttttacagac tttgaagaag
    35161 acttaatgga aaaaatagaa aaatttttaa gagatagatg ttttgcagag aatctaaaat
    35221 ttatctatat ggaagaattt gaaatatctg aaatactaag gaaaagtaaa gaacttgtta
    35281 cacatcaggg atttactgga atagcagtgg atacatttaa atcagaagat tcgtcagatg
    35341 cacattatac aggaaaatta attgaaaatt ctaaattact cgatagtttt gggaacaaat
    35401 ataatgtaat aactatgtta tctatgcaat tacttacagc tcaagaaaat aaaagctcat
    35461 atctttcggc aggagatttg tcagaagcga aagctgtcaa aaatgtatgt ggtttattaa
    35521 aacttatgag aaaagtagta aatgagttag aattagattc tactaacaag aaattctttt
    35581 taaaaccata taaattaaaa tataataaat taaaaaagac tactgaaaga gaatatatcc
    35641 agtttgatgc aaaagatttg caaaaagaat atagattatt atttttaaat aagtcaagaa
    35701 gaggtcaaga tggagatgta attttactaa gattttatgg taagactggt agattagaag
    35761 agataggaag gtgtgagaag gtctacagag gacaattgtc atactaggtg atgactatgg
    35821 aaataaagga tttgactaat gaacaggtga taaattttat ggaatattta ggttcggact
    35881 tatctcctaa aagcaatgat agacagttaa tattcaatac ttgtttatgt cataatggtg
    35941 attcatacaa attattttat tatacagaaa gcaaaacgtt tcattgctat agttcttgtg
    36001 gccatatagg tagcttaata gatttattaa tacacataaa taaatatgag tttaaagatg
    36061 ctattaatga aataaaagat ttctttggta tttcaaatca accaatgcta agaaaaggat
    36121 ttagaataaa gaaaaaagta gaacagatac atgatattaa agatatacaa atagaattac
    36181 ttcctacccc taaaaagcca tatgtatata aaacttttca gcaagtccca attgaagagt
    36241 gggaaaatga aaacatatct tttgaagtcc ttaaaacata tcaagtatat tataatctat
    36301 atgaaaatca aatagtaatt ccacatttct gttggcatga tagagctagg cctgttggta
    36361 ttagagttag aaacttagat gaagataagg ctaaaagttt tggcaaatat attccattat
    36421 ggtacgacaa taggtgctat aatcatagat taagtcttaa tctatatgga ttaaatgtaa
    36481 ataaaaaatc tatcaaaaat tccaaaaaag ttattgtatt tgaaggtgaa aaatcggtgt
    36541 tacaaatggc tactatgtat aaaaataatc cctctgtagc tatatgtggg agtaatttta
    36601 gtagagagca aaaaaagata ttaatagatt taggtattga agaattaatc atagcttttg
    36661 atagacaatt taaagttaaa gatgatgaag aatatgttat atggagagat aagatatata
    36721 aattagttca agatattaaa gatgtagtga atataagtgt aatatgggat aaatataatt
    36781 tattgggcta caaagatagt cctagcgata aaggaaagac aatatttgaa aaactattaa
    36841 aaaacagaat taatatagag aactttgtta aacaataaaa aagtaaagaa attaatttta
    36901 tgagaaaatt tttattgttt agggatttat tagcagatgc taaataaaat atgtgtttta
    36961 atatgaattg gaggtagatt acatgataaa atttttaata aaaaacgtac acatgggaga
    37021 taaattatat tgtataaata acataggaaa ttataaaaaa ggtgaaattt atacagtgag
    37081 tgatactagt ttttttggac tctttcataa aactaaagga gaaaattgct taccaattga
    37141 atgttatttt atacacttac caaaaaaaga agagttagaa agtaatccta aatcaatgac
    37201 taaagaagaa attgaagatg aactaggata taaaattaaa atagtttcta aataataaag
    37261 tgaggtgata ttatattgag tatcttcaat aaaaaatatg gtagaaatgt aaaaataaca
    37321 gaagaagaac ctccttctcc tcctcccaaa caaccaatat ataatagaga taatatacaa
    37381 tatgataaag aaatggaaag aaaactatta gatattgata ggttaaaaat agaattggaa
    37441 aaagaaaaaa agatagaaga agaaagaaaa aaagtagaaa gagtaaagga aaatgaggga
    37501 aaggaatggg cttgggttga aggatataaa tgtacagata aaaatatgaa atgtaaggaa
    37561 tttcaatatg aattaaacaa agcctatagt atggatggtg atgatattaa tatatggaat
    37621 tggggattcc atttatgttt agacttgggt gacgtatttc aatattatgc tccaaattta
    37681 agcaatagat tttttaaagt aaaagctcta gtgaaaaaag ccgataaata taaatatggt
    37741 aaagaatatg gtgaagtgac tctcctttca ggtagtgttt tacgtggtca taaaataaat
    37801 aaaatagctg ctaaagagat aatattttta gaagaattaa cttttgataa attaaaacta
    37861 catatacaaa atagttttcc tgaagtaaac actaagtcta aatggtatgc catatgtaaa
    37921 ttaggagaac aagaatatta tcatagaatg tttatgaatc aaatgaaaaa gattagattt
    37981 tcagatacat tttctcaatt attatttgat gaatgtcgta attattgtga aatattagaa
    38041 atcttaaata aagcaatagc atattcaaaa gaaaatttat ctaaagatat gataatatat
    38101 ttgcttatga aagatataag aaagtaacat ataaaaagaa gggagataat gtggacaaaa
    38161 taaataagat agatttgatt aagttaaaag aaatgaatat atatgataat tatcaattaa
    38221 aaaaaatagc aagtaaaatg atattacatc taagatatga aataatggac ttttgtggtt
    38281 gtggttctcc tgaagatata tcttttatga taaaaggagt attgactgcc atacaaaata
    38341 aagaaaaaaa ttgtaattta gaatacacag aaagatgtta catatttgaa atagaattta
    38401 ataatgtttg tggaataatt ggtagtaaaa atgatttgat acaagaattt atattaaatg
    38461 ctttaaattc atatgggtta ttagaacatg gtagtagcat ttgggggtca tggctatctg
    38521 attatggtaa acaaatatta tgtgcttttg aaatagttgg tgactgtatt ttagatgtta
    38581 gctatttaga ttaaaaagtt agttttaata ggaggtggta attattaaat atgtaatgag
    38641 tgacttacat ggtatgtatg ataaatttat ttcaatgtta gagcaaatca atttcaattc
    38701 aaatgaccat ttgtatattt taggagatgt attagataga ggggataagt ctttagagat
    38761 tatagattat ataagaaagt ataagaatat aactcttcta aaaggtaatc atgagttaat
    38821 gtttcaagaa agttatacag atgtaaatag tagttttctt tggttttata atggaggtaa
    38881 agatacattt tatgatttag gaatgaagtc ttatgaatat aaagaaaatt tttataaata
    38941 tattaagaac cttccatatt tagagataat agataatttt atactagtcc atgctggact
    39001 ataccttcca aataactatg agaatctcag tatagagcaa ataattgagt tacaagaaga
    39061 agatatttgt gtgtgggata gaacaatatt aaatactaat aaacatataa aaggatacac
    39121 aattattatt ggtcatacac caactcaaaa cattaaagat tatgaaaagg ctgaaatata
    39181 taaacaggaa ggaattataa atatagattg tggagcatgt tttgataatg gtaaattggc
    39241 ttgtttaaga ttggatgata tgaaagaatt ttatatttaa gaggtgttat atggacatat
    39301 taaaaatcag ttacaacaat ctaaatgcta tatcaaaaat atcagaagta ttaagggata
    39361 ttgtaaatga ggacacttta atagtatgta taggtagtga tagagtttta gctgactcat
    39421 tagcccctat gattggcagt atactagaaa aatctactat tagaaataaa atatttggag
    39481 tattaggaga ttcaatacat gctttaaatt tagaacaaaa aatacaagca ataaaaaata
    39541 attatccaaa ttcaaatata atagccattg atgcttgtat aagtaaaatt tcagataaag
    39601 gaacaattat aattagcaat aaacctgtta aaccaggttt gggggtagat aagaagttac
    39661 tagaagttgg tgattattca atagttggga ttataggtag aaataaatat gatatatatg
    39721 atacttcaga ccctgaactt atacttgatt tagctgatgt tatatcgaaa tctttaatat
    39781 ctatattatt agaaaaagaa gaaaggatga ttgtatgata caggaagtca gacaagaaat
    39841 actattaaaa aatggtttga ttttatatac aggtgatttg gtagaaataa aatacaaatc
    39901 agatgaagat gtaatagaac atacttgtaa aggtaaaatt aaagaagcaa aagaattatt
    39961 tattaaatta gatacaagta agaaatataa aacttctgaa agaatgattt attcttggga
    40021 attaaaagaa ataaaaaagg tggatgatga agatgaaaaa gataattaat agtaaatata
    40081 gagcattata tttatttata ttttttctaa ttttcaattt aatatatagt ttatattttg
    40141 gaaggaatac ggagattgga ttttattcta gtacatcttg catagaagaa tatatactgg
    40201 atattataac ctctatagga ctatttttct caatgatgtt agctgggttt gatgttacag
    40261 aaaactttat aaacagttta agggattatt gtgaagaaga tgcaaagtga ggtgaataaa
    40321 ttggaattaa aggattatat aagtaaagta gaattaaatg acatgccatt gaccttagac
    40381 aataaataca tgttaaatga aataagcata tctgaagata gcaaaatctt tgctgtaaaa
    40441 gacacatcag gaataacatc ttattatact gaaatagaat ttaataaact atttagagga
    40501 attaatagca tcctagagaa tattatctaa tatatatttt taaggaactg agggttgaga
    40561 gctattaatt aatagctctc tatttttttg aaaggggaga attaatggaa aaggaactat
    40621 acagctttag taaattaaat gcatttaaca catgtccatt tcagtactat cttacatatg
    40681 taaaaaattt aaatcgagaa caaagttgtt acggttatta tggcaatgaa ttacatagtt
    40741 tactagaaca attacaacaa agaaaaataa caaatcaaga agctattcaa aaatacaatg
    40801 aagttataga atatgcaaat ctaatggatt acaattttcc tacaccaaat tcaagaatta
    40861 attatttaga atgtatatta cattatatag aaaattttgt accaattgaa tgtgatagat
    40921 actatataga agaatatttt gagtttgata ttaatggaat tacaatgagg ggatatatag
    40981 acttatatta tataataggt aataaaattt atgttataga ttataaatcg agtagtaaat
    41041 tttctaaaaa agacctacct aaaaaatcaa tgcagcttat cttatatgca atgtatctta
    41101 aagaaaaata tccagataaa ataattgaat atgtagcttt tgatatgtgt aaatatatga
    41161 ataatgaaaa aggagtatta attgaaagaa ataagattga tagtataaat gattatgaaa
    41221 gagctatagt aaaaattaaa tatactaaag aactagagaa acaattaatt gattttgttg
    41281 ttgatacagt tactcaaata aaacagctag attccaacga tgagagcgtt tggtgcaaaa
    41341 gtgatgacaa aagtaatcag tttttttgta aaactttatg ctctcattat aacaagggtt
    41401 gcaaacataa ggaaaacaag agttttagaa aatcaaaatc ataataaaat gtagatttta
    41461 ataagattgg agagtgttta aatgaatata ccaaagcaag taaaagtagg aggtttattc
    41521 tataaaatag aagagacaga agaacctatt ttagtaaata atcaattatg ttatggatta
    41581 gctgattatg gaactgaaac aataaaatta tctaataatc tacaatcaga aagagtaaaa
    41641 gaagctactt tcttacatga gttattccat tgtatattta gagatagatg tatagagagt
    41701 gaagatgagg aatatttagt agatgtatta agcaagggac ttcatcaagt gattattgat
    41761 aatcctaata tatttgaact taaggtgagt atagatggat aaattaaaaa atatatttgt
    41821 atttttaggt tattctggct caggtaaaga tagtatagta acagaaattt caaagcaatt
    41881 caatattcca attctaatat cacatacaac aagacctccc cgtaattact tagaaatcat
    41941 taataaaacc tattattttg tagataataa attctttaag aaagaaaaaa ataactttat
    42001 agaaatgaga aagtatatag ttcatgatgg cagcacttgg ctatatggta tacataaatc
    42061 tgaattagag aataaaaaat atgctttgac tatagtagat gcaagtggat ataaagcact
    42121 agaaaaatat tttacaggaa caaaaactaa gttagtacca ttttttataa acgcagatga
    42181 agatactata agaaaaagat taatagatag aggtgataat cttaaagaaa tcgaaagaag
    42241 attagttgat gataagatta aatttaaaga gtttttaaat agtgaaaact acattgtaat
    42301 agcaaataac ttaagtataa ctaatgcagt agagcaagtt aaaatacata tgaaagaggg
    42361 gattcaatgg tcttagcctt agatgtatcc atgtcctcaa ctggatgggc agttataaat
    42421 agaaataaga atatattaaa atatggaaaa atagtaacta aaaaagataa attcaagtct
    42481 gaagatgagc gaatgtgtta tatctgtaat gcaattcaag agttaattat aactcataat
    42541 attcaaatag tcttaattga agaccaattc acttcaagaa actctaaaac aattcttagt
    42601 ttaagaaaac tcttaggagc aataatgagg actgtaaaat taaataatat agagattgaa
    42661 tatatgtatc cagtttctat tagaaaatat ctcctgaata atggtaaagc taaaaaggaa
    42721 gaagtagctg catatgtaag agaaaacatt attgatattg gagaatatat tgataggact
    42781 tgtaaggcta aaacaagtga tatatatgat gctatagcac ttggaatagc ttatttaaac
    42841 aaacttgata ctaatagata attttaaaaa gggagattaa attaatgagt ataaatgtta
    42901 aaataaagaa attaaatcaa aatgcaatag tgccatctta tgcaaaagaa ggtgatagtg
    42961 gaatagattt atatacacta aaagatacgg tgattccagc taaatcagca gtagcaatat
    43021 ctacaggtat atcattagaa attccatatg gatatgaaat gcaagtcaga cctagaagtg
    43081 gtataagctt aaaaggaatg ttttttacaa gatatgtgac tatatatgat aaagtgcaac
    43141 ctaaaaaaca aattagaaaa atttttgtag cacatccaac tgtaagatta ggtacagtgg
    43201 attcaggata tagaggtgaa attaaaataa ttacatataa tgaagaggat tttgatattt
    43261 taattcctaa acatactaaa cttgcacaag gagtatttca acaagttcca agagctatat
    43321 taaaagtggt agatgaattg actagctctg aaagaggaag taatggattt ggaagtacag
    43381 gtatatagat gaataagtat ataatcctag aaaaatataa ttattactat gatgacaaat
    43441 gtatgacatt atatcattta gaaaatatct atacaggatt aagattttct attgaaaaaa
    43501 ttgaattaga gtactataat atatcctaga ctaactttgg gaggagtatt aaatgtacag
    43561 atatgatgat tatgtaaatt taataaatga cttagaatta ccaaaagaaa ttaggaataa
    43621 atgtgttaga gttataatga cactagagaa catgcaggat attatggtag tacataagtt
    43681 taagacttat gttgttaatg aaaaatatat tgaaaaagta gcataaaata attaagtcat
    43741 ctattaattt aggtgacttt tttgcattta tgtcgaatat ttcatctact tcatattgta
    43801 ttagatgcta taattaaaat atatgtcgaa aggtagatgg tgaggatgtt taaggagata
    43861 tttgaaaagg tatttatgtc agaagaagtt agtaaaactg taaaagaagt taataatatt
    43921 attaaggata ctaagataca agtaaaaggt ggatttggag aacttagatt agattttata
    43981 cttagcagat taggcaaaga ttacataata attaaagaca ttattgtacc aggctcaaat
    44041 aagactacac aaattgatag tattgtgatt tctatgtatg gaatatttgt tatagaatgt
    44101 aaaaatttta gtggatatat ttacggaaat gataaagata aagtatggac tcaaattgta
    44161 ggaaaaacta aaaatacatt ctataatccg gtaagacaaa attatgctca tattaaagca
    44221 atagaaaata taataggaaa taaatataat atatattcaa taattgtttt ttcagacaaa
    44281 gctacactaa aaaaatgtaa tgataagtgg aaataatatt atagttataa atgaaagtga
    44341 gatattaagt acaataagta aatgtaaaga tgtgactatt gataaagaag aaataaaaat
    44401 tatcagagat aaaatttttg attgtatgaa aaccactaat caaaatacta gagagcatgt
    44461 tagaaattta aaaaatatta ctgaagagga taagtgccct agatgtcaaa ttggacattt
    44521 gattaaaaga aaaggtcagt atggtgaatt ttggggatgt agtcagtatc caaaatgtaa
    44581 atacactaga aaataaaaaa caacctctta aattcgattt taaggctttg taaaaattaa
    44641 tgcttataat taatcctgtt cggaaaatcg attgcttaaa atggaaaata tataaaaata
    44701 atagcgtgat agtgtaaggc atagaattgt gtattctatg tcttatttct ttataattaa
    44761 gtaatttcaa taattatata aataaaagaa ttaaaaaagt cagttgaaag tcagctaaaa
    44821 tgactatttt ttgcctcctc tgtatggtat aataagtata tgaattttca taggagggat
    44881 aaccatgaac gcttttataa gaaaaagaaa taaaaattat gtagtgtact tagaatttag
    44941 agatgatgaa tcaggaaaaa gaaaacaaaa aaatatggga gcatttgata aaaaaagaga
    45001 tgctaataaa agattggctg aagttaagga cagtatatat aaagatagtt ttcttgtccc
    45061 aaatgaaatc actctagctg aatttttatt agacttcctt gaaaagtata aagataacat
    45121 ttcagcatct acatataaga gctacatcgc tatttgtaag aatcatatta atccttctat
    45181 cggaaaatat cgtcttcaag agttgagaaa tattcatata caaaattata tagatgattt
    45241 atctgggaac ttaaaccctc aaactattaa agtacatata aatgtattga gacttgcaat
    45301 aaagagagcg tatagaatta agttaataaa agaaaatatt atagatggaa tagagagtcc
    45361 aagaattaaa aaatttaaaa atgaaattta tgataaagag cagatgctaa aattattaga
    45421 agtagctaaa ggaactaatc ttgagcttcc tattagttta gctataggtc taggattaag
    45481 actttcagaa gttttgggat taacttggga taatattgat tttgatgaaa atacaataac
    45541 agtaaataag ataactagta gattagatgg ttctgttata cttaaagagc caaagacaga
    45601 aagctctgtt agaaaaatat ttgcaccaat agagcttatg aatttactaa aaaattatag
    45661 gctggagcag aataagaagt tattgagaag tattgttaga aatgaatata acttactatt
    45721 ttttgataga aaagggaatc caattgctga agatgtaatg agtaagaaat ttagaaaatt
    45781 cttagagaac aatgatttac ctcatattag atttcatgac ttaagacatt cacatgttac
    45841 tttacttata aattctaaag tacctataaa ggttatatct gaaagggtgg gacattcaaa
    45901 tattaatact acacttaatg tatattccca tgtgcttaaa gaaatggata aagaagcttc
    45961 agatagaata tctgaaaatt tattcaaagc taattaacaa ttattatcaa taaatacaaa
    46021 atcaatgata aaaacaaagg tataaacata aaggaaagtt tttacaaagc cttagaatgg
    46081 catatagagc gtcggatttc agaagatatt aggattataa ctttataaaa aataagttat
    46141 aatcctagcc aatatctact atcttaatct ttatactaag attttttatt tgcaaaatat
    46201 gatgctataa atagtatgat agaaagaaga aatcctaata atacaatacc agatataata
    46261 ttattctgca ttttatttat gtttaaaatt gataagacta catttgaaag caacatgact
    46321 atattagcag ctaaaaaagt agttcctttt ttatttgtat aacatataat gttcattata
    46381 catataatat acaaaacgat gcttgtaatc aagtattatc agctcctaat ttatatttta
    46441 taagacaaac gctagtaatg ctttagtaac ccaccatgca acatcattac tcattccaag
    46501 acttttacaa cctttatata atccatcttc aactgtaccc gtgaatgtat ttaaaacact
    46561 aagtattcta ccataagctg catatcctcc tatagcaact ttaacttttg taggaatttt
    46621 agtccaagca attcttaata tttttacagc ccaacttaac tttcctcttg tagtaggtgt
    46681 agattctgct tctaacttta cttctttaat agcattgata taatctgctt tctcttgttc
    46741 agtaaaacca aaataagctg caatttcatc aatatctttt agtttttgtt tgtctaatat
    46801 gtatgattct attcctgttt gatgagagtt actatcagca aaagaaaccg atggtaataa
    46861 tgtagtaaat agagttgtac caattagaga agtagctatg atttttttgc attttttgtt
    46921 catatatcaa acctccaaag tattttaatt aagtacctat tttaattcta ctctgatagt
    46981 ataaaatatg caagaaatca ttcattaata attcttaggt attcataaat aaaagttaat
    47041 tatttataag tatttcttaa ttatatatat gaatatataa ctataattat aaagttgaat
    47101 tttctataaa gctttagaaa atgaagtatc aacgtcagat tttagaagat attaaaattt
    47161 tatatttcca atatttatga tataataaac atagcaagga ataaatattt gaaaaagctg
    47221 tgagtggtgt ttccatagaa atttcctcac tttcttatga aaggaggtga aaaactatgg
    47281 aaaatctaat gatgagtatt atagctggtg ttatagctag ttatatctac gataaattaa
    47341 aaagccactc tgagcgacca actaagagtg gctgggaact taatattaag ttccataaaa
    47401 acaaacatta ataactaatt aatggaaaca tcactcaaag ttaagtaaaa tatgtttcct
    47461 tgcttttatt ataccacaaa ttaagaatat taaaacaaaa tttcatgtaa ataacattta
    47521 atctgtaatt tttctatttt caaaaaaact aactaaaagt tgaaatataa caaatccaac
    47581 agttgagcct aaagcattta taggtactga tgttatatta gtaaagttat ttaaagctga
    47641 tatccctagc attactatag aaactatagc caaataagag ataactaaaa aaattggtat
    47701 tgatatactt atattaatct ctaattcagg caatttcata atatcattcc tttcaacatt
    47761 cttatattta atcttattat accattaaat ataagaaatc ataatgtaca aaataactta
    47821 tttttaatta catactatga aagagagtaa ttttaatagg ttttatgttt tatatatact
    47881 attccttaaa tgggaattta aagaataatt gaaattttga ttttaatata atttctttaa
    47941 tcaactctaa catacaaaaa taagactctt ttcaatatta actaaagagc cttattttat
    48001 aattgattat attttagtta acttttgtgt atcaaaacta tactcatata cttctaatgt
    48061 ttcagaattt acaattagag ttcctactgt tttaatacta tcttcttttt catcagtata
    48121 agtatcagaa ccatcagtgc tagattcatc ttgtttaaca tttttaatac tgattatttg
    48181 aaattcatag tatggaaaat ctatattttc agctttttta tatcctgaac tatcataatt
    48241 atatgcataa caataatcat gtgcatttga accttttgct ttatatatag catttaaagc
    48301 ttttgatatg tcacttcttt caatgctacc aattaacata taatctggag tcaggcattg
    48361 gtctaaaatt tcatttttta tatcacctat tattattggg taataagcat tagtagctaa
    48421 tgatttgtca ttagtttcat ttactaaaaa tacaggatat tctttgtgta ttgtagaaga
    48481 taagagtgca tctattaaat tatcagagct tacaatacat atttgattta gatatttata
    48541 gttatcaatt atttctttat aaaaatgctt tattattttt tcattagtgt caagtctgtc
    48601 aacaccacca attctagttg ctttcgtact gttcactaaa gagtcgttta ttgatgctgt
    48661 tcctccaata gcatatgttt ttaccccatc tgttttaaat ggtatattct ttccatttgt
    48721 taatacaact ggatttttat atttagcagc aactggagat atacttatag aatcagcctc
    48781 tccttggtaa gcatttgtaa gatatacttc atcaaccttt tttagattgc ttatttcttt
    48841 tgcaacatta taacttgtgt ctattctgtc ttttccttct atcctaataa cctttatttt
    48901 tttgtttttt agtatatttt ctacattttt acttatagaa ttaactccac ctattaaata
    48961 aattttttta aatttatcta atcttttaag agtagaattt ggtatagaat tttgtttagt
    49021 aagtaatatt ggcgagttag aagctccagc taaaccacta gcactcaacc catctgcaag
    49081 acttttatct gtgtttataa ttattgcttg ggtgtatttt cttctgtctg ctattaaacc
    49141 tgctgtttca tacttatttg ctccttgtat tctatgagct ggaggtctaa attttagttc
    49201 tttagctgaa actaataatg tgctagacat gacaatagaa gatattatag ctaaggttaa
    49261 tgcttttctt ttgaatttca tattgtaaaa tcctcccatc cttttatact atgaatgata
    49321 gtgtatatta ttttagttgt caactctcta tgtatatttt cttatatata taagaataaa
    49381 ttgtattaat tatatcgtta cgaaaacttt ttgtatataa taaaagttca cttctttatt
    49441 tattcttata acaatcctct aaagaactaa ttaagctttc tatagaatta gaatgtactt
    49501 cttctaaaaa tctctttgtt tcaaatattt tatattcttc ttttgtaata agtattgtat
    49561 aatgtgtaat tctttttatc ctctcagaat ctaaaaaacg tttaatagtg agtcttgaaa
    49621 gaagagttag tgtagttgtc gccttccagt tatattctga ttccatagct tccataactt
    49681 cttttgatgt tactatatca ttcttattcc atatgaattt cataactttt aattcagctt
    49741 gtggtatttt ttgcatggat atgtacattc ctttctgcaa ttaaaatatc aacatatata
    49801 gacgttaaaa taataaaata tatgttataa gtatatatta acataaagta aaagaagatg
    49861 tctcaatctt aagacatctt ctttattaaa tttttattct tcaaattttc gatactcaat
    49921 tatttctttt atcaatctaa ttatcgcttt ttgttctttt actgttaaac tatcaattaa
    49981 ctcataggct tcagacgaaa gagtttcaac atcattttca ttctgtataa tattttcaaa
    50041 taaaacttct aaaggtatct ctaatgcatt tgcaatttta aaaattgaac gaagtgatgc
    50101 tttactttca cctctttcaa tttgaccaat ataagcagga aacaaaccag ccttctttgc
    50161 taatgtattt tgattatacc ctgcttttct tctataattt cttattcttt taccaattac
    50221 attatatatt tcatcttcta acatttttat cattccttat tagattatat tagtttattt
    50281 atcttaatga tatgttatac atgtcaaaaa tatacttata taacatgtaa tattattaaa
    50341 tatatattat ataagtatgt atatatttaa taagaagcta ttgtagacaa aaaaaataaa
    50401 gccctttaaa gaaaactcat ttaagagtaa tccttaaagg gcttattatt tatctatctc
    50461 tttttcttac cttttttacg acttttacat gccatgatat cacttccttt ctactttaca
    50521 tctatttata aacctatcat atctttagac ttagaaattt tgcctttgtc ttcttttata
    50581 taaatcttag ttgtatcagt ggattcgtga ttgaggatag ttgatatatc ttcaagtggt
    50641 aaattattcg ctttagctat agttgcaaaa cttcttctta atgaatgggg agctaagtta
    50701 tctatatcta ttaatttacc agctttctta gcccaacttc ttaatacatt agatgtagcc
    50761 ttatgatatt ctttatcata tttaactaag aaaacataat catttactat atcttctttt
    50821 tttctaactt ctttaagctt tagtaataaa tcttttactt tctcactaaa ataaaaatct
    50881 actattttcc cacctttttc tttaatattt tcacatactc tattatcaaa gtctatatta
    50941 tcccatctta aattacttgt ggctgtaact ctaccagcag ttgatagagc aaaatttata
    51001 taaacctcta attgtaaatc atctcgtttt gataacttat cttgtagctc tttagcctgt
    51061 tctatattca agaaatgttt ttccacaaca ggtaaaccct ttttaggtct ttcgattaga
    51121 gatactggat tctctgtaac aattctcttc cttctaagat ggtcatatag agatgaaatg
    51181 cctgaaaata ttacttttat tctattaaca tgattgtcct ccatcctaaa tgaaatatat
    51241 tccattatat cgtcttcagt acaatcttcg attaataaat cctccatttc atcatgcaga
    51301 tatttagccc aagtatataa gtcacattta tatacatata tagttttttc acttaaacca
    51361 gctattttct tagcttgaag aaatccttta attaactttt cattattttt atttatgttg
    51421 ccttctatct taattctttt acttcttttt tcaaatgttg ccaaatactt cacttcctta
    51481 taaaaaagac tagagaaatt aatctctagt ctctaattta tctttaattt ttgttatgtc
    51541 tgtttcaact ctttcaagtc tactgttatt agtatttaat gcttcagcaa atttatctaa
    51601 tttatcatca aacttatcta ttgtattacc aaataaagtt ctctgctctt taatttctct
    51661 tgaaaaataa gctctttctt tttttctatc atcctgctct agttgtctat tcttttctaa
    51721 atatctgcca aaccataaca ttactactgt aagtagtatg gctgtatatc catattggct
    51781 taataattca atcatttctt tagataacat ctacttttcc tttctgtttt gtacttgtat
    51841 ttgatttatt cctatagatg ctccccaaca taaaatacct tcaaaaacag cttctattga
    51901 cagctctttc ataaacaaac aactgcttat cattgctata actaacaata taaaaggtat
    51961 gtatttattt ttaatattag attcttttat aaatgttcct aatatgtaaa gcccacctat
    52021 taacattatt aaattttcag gtataaattg cattatatcc atgtgatttc ctcctatcca
    52081 agaatctcat ttaattcttt tactttagtt tttatttgct ctaattttac atcattgttt
    52141 tctaccttca ttgaacttat cttattacat atatttatta tgttttggcc ataattcata
    52201 tctggacacc atttgcctga taaactttct actgttttag cttctccatg taaatatgga
    52261 aaatgtctag ggtctaatgt agttccattt actttatatt caactttacc aactaattca
    52321 ctatattttg gatatccctt agctccagca tataatgcta gatggtcaat aaatgctgat
    52381 actccatcct cccaacaatt aaatcttgta tgagctgttg ggtctaaatc tcctccacct
    52441 ttatttcctt taagaccaca cacattgcaa taagaaggat ttaaaactct accaaaatta
    52501 aaatatccag tttctacaaa tgcttgagca attgcaataa ctggatttac accttttttc
    52561 tctgcttctt tatatataat tggtactata gctctgcata aaaaatgtgg tggattagag
    52621 ttatttttta atgtgtctaa atacgaaaag acttgttcta atgtagctgt tgatttatct
    52681 aatatattca attcattacc tcctacttgc ctataaaatc taacacttta tgaagtgtag
    52741 cccatctatc atcaccttgt aacttagtga atctctcgct agttttaatt ttactggatg
    52801 ctctaccaat cacatatagg ttttcacaat gtccaggaac ataatttttc aactcacata
    52861 tagagacttc attagattta taattcatgg ccattaattg ggctataact ttatctactt
    52921 caccttcata aactattgcg tatttcactt tgttatcttg tggtttacca gctacagact
    52981 tattaaatat tccttcgtaa atgtctatgg ccatatcttt agcattatat tttttagtat
    53041 cagatgtgtc tacaaagcag cactcgacta aaattgactt tgccttcgtt cttcttaaaa
    53101 atgctagagt tttcttttct ttaactccac gatttttaaa atctttatca ccttttgcat
    53161 gataagtttc agttattttt ttacatattc ttgatgctat ttcaacagtt tctttgtcat
    53221 ttaaattata cactaaaact tctactcctc gaccaccacc actgttgaaa tggatactta
    53281 catttaaatc tgtgttatat gaattacatt tatctataat cttattcaat atatcactct
    53341 gagaagttcc attgttacat gtacaatcat aaactttaca ttccttttga gataaaggta
    53401 ttaactcttt tagtacattt cttgcttctg tagattcttt tatatttcct actgctccag
    53461 aacctacttt accatctggg ttgtgtcctg catgaattgt taatgttttt atattcaaaa
    53521 ttaccactcc ttttaaataa ttaatataaa aagagcctat aaaacaaggc tcttattcac
    53581 taaatttcta attaaaatta tgattttaat atgtttttat taaataatct aaaaaagaac
    53641 cttctataca attggttctg cttcttcgac tactccacta tgctctataa tataatcctc
    53701 tactgctttt ctgtacccta tgttagtcac gtcatctaac tgaaattctc tatttttcaa
    53761 tgggtttaac cctccgttta atattctctc tgctaatatt cttactacaa cattatttat
    53821 attcattata aaattcctcc tactttttca ttttctgcaa ttagtaattg attttctaac
    53881 tcttgtattc tcttttcttc ttctgaaaca aatactggta tttcttctaa gataggttct
    53941 tttgtttcta tatttatgct tacaattctg cttttagtat aatctacact tccatacgga
    54001 atatcaacaa aatgtaattc cgttatttca tcatgttcta atatatcccc tgttgcttct
    54061 ccagtttgga gtaatatttt cccggtttgg tcacatataa ttctatttgc tctattcatt
    54121 ttatcacctc atttatataa attttatagc atgccaagaa tgtaggtaac tagaaacatt
    54181 aggactactt gcaggaacat taatgccttt attatttata tatacatcac actcattatt
    54241 tgaataaata attccacttc cactaaaatc ttgattagaa tttttttctt tagtaaatac
    54301 aattctagct gaaaaatctt ttttatccca aaaccaattt atattacaag tagcaataac
    54361 aatatgttta aaaaaataat tattagaagt aacatattgg cattcagcaa caaaaatatt
    54421 aggaataaaa cctaaattat caatcttaag ccaagtacca ggttgtctac caaatgactc
    54481 tccatacaaa ttagccatta aagaactatt tttaacaaca tcaatagtac cactggcata
    54541 tttatactta gaatttaact gtgatatagt attattagct tgtgttaact gattcatcaa
    54601 atcctgcgca ctagcgtctg aactatcaaa acttgttttt attttctcag ataactcaac
    54661 taaggtatta tttaaacttg cttctatgtt ctttaatgct aaagtgttta taatacttgt
    54721 tttgccactt ttgaatgtat ctttaatctc tatccatttg tttgtaactt catctgttgt
    54781 agaaccagca ggaagaggtg caattccttt gcttatactt acaacttttt ctgctgtagc
    54841 attggcactg tctgtaacaa ctatcttaag tgtgtgtagt gcgttatctt ctaatgtgta
    54901 gttaattgtt ttttcaaggg ttaaatctgt tgttatagtt tcttttaata catcatctat
    54961 aaagtattct attttagtta ataatgtagg gtctgtgtgg tctgctttaa atgttgctgt
    55021 agttgagtta taagatgata ctgttaaaaa cggtaatgct tgaagtaatg ttattttagc
    55081 ataaccatct gctttagtag tattacctcc agtagtcatg actatatttt caagataata
    55141 ttcagatgtt ggtatatatc caggtggctt ataactatct ttagttagtg cgtaaccact
    55201 tccaccacct ccacattcat tagaataaga accagcacca ccataccaac caccaccgcc
    55261 acctactcct attccaccat aacctccttt tcctaacgaa ccatggtatt cttctgtatc
    55321 gtaacttgtt ccaccttggt attgagaacc accaccacaa aaatctctgt cacgaccaat
    55381 tccattttca cctacataac caccaccatg accaatagaa tgagcagcgg cgtaattatt
    55441 tttcgtccct ccaccaccac ctgcaacaag tatacgcgaa agtaaacctt ctgcactatc
    55501 ccaagtcgct ctagtataat aagctcttat atcggttgct ccaccaccgt atttagaata
    55561 ataattacca ttaataccat gtgcatttgt tcgtcctccc ccattgaatc cagttctggt
    55621 aagagttgaa ccagtatcaa gtttttcata acccgattga ccaacacaga tacataagtt
    55681 agttcttttt tttaatataa cttcgccttt tgaataaccg ccttttgcac attcagtcca
    55741 atcacttgca ccaagagtac caccacatgc accccaacat tctagtttat atcgcccagg
    55801 tggcaatgaa acattttgta cataattagc ataattaaaa ttccattctg tctgcattct
    55861 ctcactctcc tctcttaaat aggtaacata tcattttgta tagatatatt aacctcatta
    55921 aatttttcta tttttctaac aacttcatct attgcccctt gaacatttgt agcagtaaga
    55981 ttacttgttg cattattata acttgttttc tctgctgttg tttctatgct atctacgcta
    56041 gtttttacct catttaatgc actaactata tttgttttat ctgttgtagt aagttgtgtt
    56101 gtatcaccta ttttgttgtt aatctctgtt ttagcagttt ctattgcatt attagtgtcg
    56161 ctaggtacat tctttaattt ttttactacc tcttcattta acaatttatc accttctttt
    56221 taagttagag agctacatta gtaactctct attttgaaca taaaaaaaga actcttacaa
    56281 ttctatgata atgaaatctc taatgcttca cacatttttc ttacttcttc tctgtatttt
    56341 acaggtactt ggtcaattgt ttttaaacct ctctctatca atcccacata aattgctgcc
    56401 atactacttc ctccttcatt taattttata tttcttaatg acatagtaga tgtctgttga
    56461 ctacttaata ccatttcaca tatttcagca gttgctaaca ttaaatctgt aatgtcatta
    56521 tcttgcgttt tttgacttat atttaaatta cttattttct ttttctgctt aagcttttca
    56581 attttttctt catttatatt tacgttcata atttaatccc ttctaaagtg tagtctcatc
    56641 tatagaaaat atcatagatg gtctttttat tgctgtatct tcatttccat ttacaataat
    56701 tttaggttct acttctttaa ttccattttc tttaaactca tatatttctt gataacctgt
    56761 gtcaactttt ttcatttcta gcaatttagc atctttatgc ataccagtgt tattagtttt
    56821 tatttgaaca tttaacctat tgattaattg atatattata tcacccacat taacatttcc
    56881 ttctaaacct ctatctaaat ttactgtgac cttaccctca gattctgaaa cagattgaac
    56941 agtatatctt tttccattaa ttaataaact tgaattttca gatacatata tagaattatt
    57001 ttctttattt attgtcaaag aagtatatat atttccttta aaattatcat atcctttaaa
    57061 tacaatatta tttctcccaa gaactaattt atctaatggt atggtaaatt gagaatattt
    57121 atttgtaaat tctgtcatag tagtaatcaa ctcattattt atatacattt ctatttttat
    57181 aatttcatta ccatcatcat ttatttttaa ctttattcca gagattgttt tttcaataat
    57241 ttcaatagtt gaattaactg aatttacttt ataaattaaa gatacagttt catcatttac
    57301 aggattaacc catactaaat tttctttgta tatactagaa ggtgttttaa tttcattaaa
    57361 aacatatata ttgttttctt ctatattata acaatctctt attctaccac taatataaac
    57421 tgtattatct gtttttctaa gtaaaatata attagtgcct atttctacat cttccacatt
    57481 tgtagcaatt ttagtgaata aaggtattat agtatctttt ttctcttcac ctaaaccaaa
    57541 gttaccaaat ctattgtatc cttgtgcata caattcattg ttatttaata aagctaatgt
    57601 aaaattatta tcacacatta ctttttttac attagatatt gttgtaatca gttgaggttc
    57661 agaaacatca ttattatgac ccagtcctaa ttgaccattt gaatttaaac cacaagaaaa
    57721 taaagctcca tcatttctaa caaaatatga aaatccattt ccacatgaag cttgtttaat
    57781 attatcaaag tttaacttag taaactctag caaagaagtt gttgttaatg gatttaataa
    57841 ttgtccataa gtatttttac cacatccata taattctcca tcagtattaa caattaaaga
    57901 atgtgtttta gacatccaaa attcttttat attattgaaa ggcaattcta atacttcatt
    57961 agcataaggt gaaacaggat attgagagcc tagtatttcg ttgtaattaa aatcatcaga
    58021 cgaagttaca taatgttgat atttagtatc ttttgtaggt attaaaaatt tattatttac
    58081 taaattaaaa ttattattga ctatacaatc tgttattttt tcaaagtcaa aaattttaat
    58141 attaacctta gtaaaaatat ttctgttagt aacatcaccc aatcctaata atccatatgt
    58201 attttgtcca gtagaaaaaa cacttccatc gtttttataa atataagatg aacgaccacc
    58261 tgcattaata cctttaacat tatctatatt aatcttagta aaagtatttc tgccagtgtt
    58321 atctcctaat cctaattgcc cctgtccatt actcccacat ccatataatt ctccattatt
    58381 tttcaatact aatgtatggt caagaccaca ggatatttcc ttcacattat ctatattaac
    58441 tttagtaaaa gtatctctat taacaatatc acctagtccc aactgcccct ctttatttct
    58501 tccagtagca tataattctc catcatttgt taacaccaat gtgtaataag caccacatat
    58561 tacttttctt acattattta tattaacttt agtaaaagta tttctattag tattgtctcc
    58621 taatcctaat tgaccttcat catttctccc tgtggcatat aattctccat tacttgttaa
    58681 tattaatgta tgacttatcc cacacgcaat ttcttttaca ttatttatat tgacccttgc
    58741 aaaactatct ctattggcaa tatcacctaa tcctaattga ccatatgtat tagttcctgt
    58801 actatataat cttccatcac caagtaacgc aaaaacaatt agtgaaccac atacaacctt
    58861 ttctacatta cttatagtac tcacttgatt aaatctatac ggttcactag ttaacaaggt
    58921 tttggtgtct aatgcttttt gacttgaagt attacctgta actaataact ctccatcttt
    58981 ttttaatatt aaggtataat ttaatccaca tatcacatct tttatatttt ttatatttat
    59041 tggataaaag taatgacatg tagaattgtt tccgatacct aattgaccat atgtgttatt
    59101 ccccacacca tataattctc cattactttt ttttaaaaag acactcatat aatcatttac
    59161 attagtagtt gctatcctaa aatcattatt atagtctatt tctgtactat taaattcaaa
    59221 accaattctt gtaaatgtag atgctgctac actatctgaa cctgtaccaa accttccatt
    59281 aagtctattt cctataccat acactttacc atcagttttt actaaaaatg tacaatcact
    59341 accaccagca atatatctat tattaccttg tccgattaca atatttgtag gagtaaaact
    59401 ttctattggt gtagttttat tattttctaa acctaattga ttataattat tttgaccagt
    59461 agcttttaac cttccgatag aatctattat aagtgtatta tatgattcgc aatgattgtt
    59521 acatatatga gtggtatcag tttctattaa agtaaattgt tctaatttgt ctgtagaaat
    59581 ccttcttcct aattgtcctt gtgtattact accacatcca tataattctc cattagtttt
    59641 caatattaat gtatgataaa tcccacaata tatttctttc acctttactg ctttatttat
    59701 agataattct ataaaatatt tttccacact attatttgag gttttcccta attgaccact
    59761 atgatttgct cctgcacccc atatactatt atcatttttt aatattatag agcattcata
    59821 tccacatgct atatctctta catcatctat aggaactttt gtaaaggatg ttatacctgt
    59881 actactagta gtacctattc ctaaataata acctgctcct gcaccccata tactattatc
    59941 attttttaat attaaaacat gagaacgacc acatgcaatt ttttttacat tatctatgga
    60001 gatttttgta aatttttgta gttgagaagt attgccagtt cctaattgcc cagaattatt
    60061 aagtccagtc acccatacag tatcatcgtt ttttattata aaagcatagt aagcaccaca
    60121 aacaacatct cttatattat ctattggaac tctagtaaaa taattttgag ttactttatg
    60181 atttaatcct aattgtccat ctgtattatt cccagatacc catagttcac cattttctag
    60241 taatgcataa gtcgaacctt caacagatac aatttttttt atgtttccat aacaaatata
    60301 atccccattt tcttttataa gatagttttt attgttcatg gtaacagatt tattatatcc
    60361 caacacctca gcacttttat attctgtaaa tacgtctaca ggataattta tattatttgg
    60421 attgcctatt tgtgattctc tattagttcc acagaataaa agtttattat catttcttat
    60481 cataatcgta tgattatttc cgcaatatat gtcctttata ttatttatac caattttaat
    60541 gaatgtttta ggattaggaa catttctcaa acctaattgt ccatatgtat ttcgtccaca
    60601 tccatataaa gaatcatcat tttttagaat tatagtagaa tttattccat atacaacttt
    60661 ttttacatta agagccatgt tggtaaatac atttctattg gtaatatcac ctaatcctaa
    60721 ttgaccttct gtattgtatc cacatccata taaagaatca tcatttttta ttataaatga
    60781 attgtatcta ccacaagata tttcttttac attatctata ttaactttag taaaagtatt
    60841 tctattagta ttgtctccta gtcctaattg tccatatgaa ttactaccac atgaatataa
    60901 ttccccatta gtttttaata taaaagtaaa accatcacca caatctatat cctttacatc
    60961 tgttattgat aatttaataa aagtatttgt attctctaag ttatcaaatt gttgactacc
    61021 attataacca gtaaaataca actctccatt agattttaat gctatagtaa gtccacttcc
    61081 aacaacaact ttcacaacat cttctatagg tagtttagta aaagttgttg ctactgtaat
    61141 atctccccca agccctaatg ctccatttga attttgacca acacctaata aagttccatc
    61201 tttttttaaa attacagtat gctcacttcc tattgcaata tcttttatat tggatacccc
    61261 atcaattttt gtaaatgtat ctactctacg tgaaatgtta ttatttcttg gactatattt
    61321 tccaaccact tgattatttc ctgttaccca caatgtttca tcagatttca aaccatatgt
    61381 acagtctata tattttgaag taaacatttt aacaaaatca tatttatcaa attctggttc
    61441 aaaatcctca atctcataaa tagtatcaaa atatcttcca cgctgtatta atttattttc
    61501 tttcaagttt ttccacctct atttatttaa tttcgtaaga atatttgtct aaatctatta
    61561 tagttgtacc aatagatata ttcttatcag atacagtttc tatataatta tcatttagtt
    61621 catgtatata tcttaatttt ttacattcaa aaggaattgt tattctattg aatgtaacac
    61681 taccagtagc accttgtatt ttcctatttt ctgtatctag ttttaaatct ccatcaatag
    61741 ataatatcgc tttatcatca gacaaaccat caaaccagtg tcctacttca tctggaagta
    61801 agccagattc caattccaaa taaactgcta tatctaagac tttattagaa agtgcgtcat
    61861 acttggcttt taaactgtct aattgttgac ctaagtttcc accagcctca agaattttta
    61921 cactagcatc taattcattt actgcatctg atatattttt agatattgta cttaattcac
    61981 ttgaaccaac tttattttgt attagattta tcttctctaa gttagtagct atattacttt
    62041 tattttcatt tatcttaatt agactctcat caatttttgc atcaatttta acactagaat
    62101 aagtactatc tagtgttgtt atgttatcat ttattgtagc ttgtataact tctttgtttg
    62161 tattaccatt tacaacagtt actcttacat ttatgttact ttcattaaat atttctatcg
    62221 tattagaatc tactatagta taagaattag tcatactttt cttagttgct tcatctaata
    62281 ttgctacaaa tatattttct gtaactaaat tatgattaac tataagttta aaatattctc
    62341 catcagcaac ccaatccgat tcaacataag atttactaaa agctacatta gttgcagcag
    62401 aagtcatctc agcaattttc tcattaactt tctcaaaatc atatgttaat ctatccttta
    62461 aagtgtcctt tacatttcca tcaatagttt ctctagcttg tattaactcg cctctagcat
    62521 caagactttc taattcacta aatctatttt caaattcagt tattttgtta gatacagttg
    62581 ttgtcatatc tgtcttagca gtatttactt caactatttt gtcatctatt tcttttatct
    62641 ttgtatctaa ttttgtagta cttttatcaa tacaatcttc tacattagat atagcctgat
    62701 ttacttcatc aagtttataa tcaatttgtt cttggttgtc tgtaagctta tcttcaaatg
    62761 tcttatcata taaatcaaaa atattattag ctctagtatc taagtcctga aatttcttat
    62821 ctgtttgagc ttggacatca agcattttat catcaacaat attgtctatt tcttcagact
    62881 tggcatctat catttcatct aattggagtt gacaatcagt aagtttatca tcaacttgga
    62941 tttgcatatt ctgaatggat tcttcacgat ttgcttcttg gttttgacgt gttaactcat
    63001 taaactctct ttcttcttct tgtatgtttc ttatatcttc attataaatc ctaatctgct
    63061 ctgctttatc cctaacttct tcatttccta agatagttat ttcaagttgt tcaactcttt
    63121 tagttaactc tttattgttt tttataatct cttctgcttg ggctattatt accaataata
    63181 ctccatattc atcagttgag gtgatatcat catcattaat cataccctct tcaactctat
    63241 aataaagatt tgtagtatta agtagagttc cctctttcac aagggatagt tgaaatgtat
    63301 ttataccaac acttgcaagt gcttgtcgac taagttctat ctctacaaca ccattttctg
    63361 catctacaac ttcacattca ccattaacct tttgtccatc actttttgta atgctaacta
    63421 aaactctatc atattcagat aaatctatta tttcatcttt atttttcaat attgcttcaa
    63481 tataagctgt tttaacatca ttttcattat aaataaaata gtctagtaac cttttattta
    63541 ttctataatt actaaaatct acttctatgg tatgattctt tatttcataa tttttcaaag
    63601 tatcacatcc tattgttctt tattttgttc ttctagttct tttttcaagt tctcaatttc
    63661 ttcttgttgt tgcatgttaa gtgctttata aagtattagt tcattatata aactttttat
    63721 ctcttcaaat gctactgtat atgctttttg taaatttact tccatattaa tatcctccta
    63781 aattattaat tttctcattt aattcactaa ctatcatttc caattcttct cttttttgta
    63841 tttcctcttg aagtgcagtt gctacaacag ttgtataacc agatgggcta aacattaatc
    63901 cattttcttc tccataatca tatagaaatg tttttccaac ataactatct ttaaaatcat
    63961 tagctataaa ccctatttga ctgtctgcaa ttgttctatc tttttctgct gatacaatat
    64021 aatcaaatgt tgctggttta aattcatctt ttataaaatc taaaaatggt gttggcgtta
    64081 tacttgaacg agttctattc ttaatatctt ttatgtatac tatattctct ttaaactttt
    64141 tgtcagattc acttagagta gaacgtgaat ataaatacct ccatttataa tcatcacaac
    64201 ctaaatcagt tgatgaacgc tctggataaa atgaatatcc tatacctaaa ctatctccat
    64261 ttgtaaatgt aaatgtagct cctccacatc ttacaaaagt atcatctctt tcaacaataa
    64321 agtgttcttc tccatcaaca taaatctcag cagtatcata tcctacccaa aaataattat
    64381 tgctatcata ttttaatctt atagctgatg cttgactaga accatcagac cttcttgcat
    64441 ctattgcaaa tccagacctg ctacttccaa ataatctgat tattggttca tggccagatg
    64501 gagtagttaa tatatcacat gctatagctc cttgatttac tgttagacca ctcctgctta
    64561 tctctacata atttgatata tcattaaatc caaattcaat tttatcaggt ttaattccta
    64621 caattgaacc acttccattt cttgtaacca tcattgttat atcgtctttg gttaatttaa
    64681 tttcagcagc attagtttta gtcacaccat ctactctagc tacttctaat tttatttctt
    64741 tagctgtagc atttatctgt gaaaattcct ctgatatact tccacccata cctgttatat
    64801 aaattctatc cactgtattt ttaagttcat ttatctttaa gttatattga gcatttgcat
    64861 ttatagctaa aattctgtct gaaggtgtaa ttatactatc tgaaataact gaattgatta
    64921 tagagtttag attggtactt gctgtatcca atgctgtttt tgaagtgttt attgcaacta
    64981 agtctactcc agtagtttta tcaataagtt tttgtaactc tgcatataaa ctcgctttat
    65041 ctgtatctaa taaattaagt tcaatatcaa tcttttcttt ttcttcatct gtaatctcat
    65101 tatcattaaa ataattagac atattagaac taaatgtacc taaatctgca tttaacttaa
    65161 ttatagcttt atttatattt tctcttaagg ttatattttc ttgtgactga ccaaaatttt
    65221 tagtaacttc ttttactaat atatttacat tttcagctct ttggtctata agagacatat
    65281 tttcttttat atgagatgca tcttcttgta cttctaaaac tgtttgatgt attccatcta
    65341 tatctttttc aataactaca aacttttcat tatgcttttt atcagtctca tataatcccc
    65401 aaatctgttc ttctgtattt tttcttgaat taccatcaaa tgaaatatca aaaatagaag
    65461 aatttatagg aacttctgtg tgttttccat tgtcatttac aactactaaa aattgtgtgt
    65521 ttccctctgg taatcttaca ttagaaactt ttaatgttcc tggattatca gcatcccagt
    65581 atatatattt tttatttgtc tctgtatcta ctacttgata aagtgtatta ttgtaggtaa
    65641 caattactcc agtaattcta atccatgaat ctgctggtga attatctaat atcaatttac
    65701 atcagctcct tttgtatatt tttcttagga atatattcat ctggtaggtt aattcttttc
    65761 ttctgttgct gaatgaataa atgtctttta gagtttaatg ttctcgttgc atgttcagct
    65821 ttagttaaat aatcagctat agtccttttg tcatcttctt ttattttttt attacttaaa
    65881 gttaattcta aagtatctaa ttttccattt tttaaattct gggtaaaaga tgtaaaatat
    65941 actaattctt cttctttaga ttctttgcta tgtaacacta taatatctcc catacttaaa
    66001 tctcccttcc aatgctgtct aaaattaata tctataattc tatctaagaa gtttataaca
    66061 tccagagtcc attctttagt tggaatacat tttaaactta attgtctctt gccttctgct
    66121 attaaatctt caacttttaa aaaagcatca tttgtataag tatcatagta gaggaactca
    66181 tttaactcat ctaataggac ttcattaaat attaggtatc catcctcatc agttgaagtc
    66241 tccctcttac aaagaatatt tatatctttt atactttcat ctaacttagc tatttcttct
    66301 tctaaatgtt ttatttgaac atctaatata acctttttat cttctagttc acttatttct
    66361 actgctaact tagctgaatt tacttcatct ttatgctctg gattatcata agttttcttt
    66421 atatcctttt tagcctttat agtctctatt acatgtaacc attcactact tttatcatca
    66481 cgttctcttt gtttcttaag ctttgtatct attaattctc tccaaattgg ctctcttatt
    66541 tcattcattt cttgatattt ctttatagcc ctgctaagtt cttcactcat ttctttattg
    66601 tctaagaaat aagaataatt ctcaatgtaa tcatatcctg taactgtagc tcctattata
    66661 tccatatctt cactgccttc aagcttcagt ctagttacta tatcgtcact atttgtagtt
    66721 ctttcaagtg attttatgta gttatcatga gataaatata actgaatgtt atctccaaaa
    66781 ctatctatat gatacaaatt aactaaatta ttataagtgt caaaatcagc tatacactca
    66841 aattgttctt ttagttcatt attaaaatag tccaaccagt tggagttaat actctcttgc
    66901 catcttactt tctctctctt attaccttca ctatcagttt cataagctat tgaatcatct
    66961 acatggccaa gtttccaacc tgtctcttgt ttcaaataat cattaagaga tataatagat
    67021 gtttcttcat ctttagtaaa catttgtaat ccataatctt cgatattcac atcaattttt
    67081 cctagtttaa cttctttaga tttagctgtt actgatttta acttgtcgtc tgtggtcaca
    67141 acattcttaa ttacaaagta ctctttatca ttaagacaaa taagacgttc ttctttaatt
    67201 tcattaaata aaggatttat tactttagag aattggaaac gttctgtaat atattttgga
    67261 atagtaagtt gtacttcatc tactccacca agctcacgct ttatagaatt taaaaagttg
    67321 attggaattt gacctagaaa tgacttattc atcttgtgaa tggttaatgt gtaatcagat
    67381 tttatttgtt taagattcac attaaaatgc atattttatc accttctttg tattagaaaa
    67441 gcccatcaat taagatgagc taaaatattt tattaatcta aatattgtaa caagtttaat
    67501 agcttgttac atatatgata aagcaaaaaa ccactcaaac gccaataaga gtagtttcaa
    67561 aagataaatt gatttttaat taactttaag gaaacaatca ctctttgtat taagtagatt
    67621 aaattcctta cttttattat accacagata cgttttttgt aaaacagatt cgtttacagt
    67681 taataaataa aaaatcacta gataaagacg attactgata tcaactaata catatttaaa
    67741 ataggtatat tataaatcat ttcattattc aaaattcact tcaatatctt caaggtcttt
    67801 ttcattaata tttaggtctt tcagaatatc cctttgagat attagttctt tttcatctat
    67861 atctttcatt cttatttctg cctcaataag aagcttacaa tcttctatat tatttatcat
    67921 ttgtaacacc cttaaatata taccagaata gaaaaatagc cactttttaa ggtggcatag
    67981 ataagtagta attcttatgt aaaattttta aattttctgt tatctgtatt atatctttat
    68041 tctactcctg tcgtcaatat aatattaaat ttaacatatt ggaaaattat ggtataatat
    68101 ttttagagac tacagtttag actattaggg tgatggctct ttagttaaaa tccccagtaa
    68161 gaaagggggt gagactatag agtacttagt tttgttttta ataatgataa ttatgattac
    68221 aagaataatc aatcatttga caaagctaat agatgcaatc ttaaacttga aagcatctat
    68281 taggaaacta aagaatcacc agactggcgg gtctagtgat tctgaaagta atacataaat
    68341 ctattctttt tattgttaac tttaagaact acaccctaca gcaaatagtt tgtagtctct
    68401 tttttatgtc cctatgatat aaatttatat catttatatt actattatac tccaatatta
    68461 tcaaaatagt caatgttgtc attatcttca ttgaaaaata aaaaagctat attattaata
    68521 gaataacttc ttagaacaca atattctttt acaattgtag acaatataat attaaattta
    68581 acatattggt aatttatggt ataatatttt tagagactac ataaaaaaaa ctattagggt
    68641 gaatcttcat atacataagc cttcccatgt caaatcaaga ggggaggtga tatgatatta
    68701 tgaaatatct aatttgtttg ttaacgataa taacatttct tacaactata attattagtt
    68761 tgattaagtt ggttcagaat ttaaatagac tctttaaaga aatagatgag tttaagaaaa
    68821 cgttgcaaaa aattaagaat cacccgaacg gcaattcagg tgattcttcc tctaattaac
    68881 acttcgctta tttatgaaga gacaccctag cacaaatagt tgtagtctct ttttttatgt
    68941 ctttatgata taaatttata tcatttatat tactattata ccatatacat attatttttt
    69001 gaaataaaat ctggatttta ttagagaaat ctggatttct acttaaaatc ctttgtctat
    69061 ataaataatc tatgatttaa tattttctac tatttttcta tatactctat tctctaaatc
    69121 tctaccaaat ttttccaagt ctttcatagt atccttagtt acatttcctt gaacaacaaa
    69181 gaatggtgaa ttaaaattaa ctgaagatgt tggaaatgca cttcttgaac gagaatttga
    69241 aggaggatta taattaggca tagcaaactt acttaaatct aattcatcta atatgtcatt
    69301 aatatcttct aatgtctctt taccttcttt tagtgtatca agccattctt gcttagtaac
    69361 agaacccatt gccaacatgc catcttcata tttgtttatg tattcaatga tagcatcttg
    69421 aagtttctca acttctccat caagacctac aaatttacca gaactgattg cttcattaat
    69481 taaatcaact aaattttcat ctgagtattt tttctctaac tcttctttta atttatctgc
    69541 ttcttcttgt agtctgtcag actccttatc atacatatca ttgacttggt cgtctacatg
    69601 gtcttgtact aaatcctgta atttcttctg ttcttctttt aattgttttt gtaaatcttt
    69661 aagtttcttc tgaccactta aagaactatc tctttcagct attgctattt gtttttctaa
    69721 atcacttatt acatcttgtt gttcttcata atcatcttta tatttggctt cttttcttga
    69781 atcattgtaa gcatcttgtt gctttttaag agaatcaatt ttagctttag tttccttgtc
    69841 tatttcttta agtctctctt caagttcttt tttatacatg tctctaattt tctcttgtag
    69901 actttgagct tctttgagtt ggtctttata agcatcttga atttctttat ttaatgcttc
    69961 aatttctttc ttagattcag caatttcctt attagcatca ggtattttat ttattaataa
    70021 atccaaatat ccctctgctg catcttccat tcttttaaat tcttcatcag tcatgttctt
    70081 tttaagttga tttttatgtt gaatgaagtt agttatatcg ccattatctc taaatgtaaa
    70141 tccatagcct tgtagctttt gttgtagctc tttttgtgtt tcttgaagat atgctgttgt
    70201 ttcagatagt tctttagcct tcttggccat taactctttt ttcttttcta acaactctat
    70261 ctgctcatca cctgtagcat ttttaagtaa tacatctatc attgctattt cattatttac
    70321 ttgctctaca tctttgtaca tagaagtcca tgtggaattc ttattaagtt tccacatttc
    70381 ttcttgtgct ttccttaatt cctcattgtg ttctttaatt ttattattgt attcttccca
    70441 ttcttgctca catttaggca gttctgtaaa tgtaacttta atatattctt caaggacttt
    70501 cttagtttct tccaactctt tttttctgga ttcatctttt ttattttctt tgtcaccaga
    70561 ctttgaattt aatttgtcta attctttttc tattgatatt aacttttctt catagttagt
    70621 tagatttcca gtatcatcaa acttaaagcc cattttcttt tcttttaatc tatttttaag
    70681 tctgttactt tcagctttat atccatcttg ttgttgtttt attaaatctt tttgttgagc
    70741 aaataattta ttttgctcct gtaagtattt aatcttctca tcacctgtag catgttccat
    70801 ttttttgtct aatagagcta tttcatttgt tactgctgat atagcatttt ctaactcttt
    70861 aaataattct atgctatatt ttacagatga gtctattgtg tcattgttta ttgtaacttt
    70921 aggtttgatt actttaaaag ctgatgtggc tcttgatact gctcttgaga ataaggatgg
    70981 ttttgttaca ggagtttcgt cacttattgg tgctggttct gatgttctag gttgggatag
    71041 attagctgga attggagttc cattagtaac agaggtacgt tcagttttat tggtcttatc
    71101 tgaactattt cctccacctc caccaccaga actattggtt actttattag ttacaaatgt
    71161 agtagtgaat gtttttcctg catagtttga tttaaatgac tctattttcc taataagccc
    71221 tgatatgttc tttgcagcag ttgcagttaa tgcactaaat gtagtcttta tagtttttcc
    71281 accatatcgg ctaacataac tttgtacttt ggctaacaaa cctgatacat tttttgctgc
    71341 ttgtgcagtt tcagttctaa atacaagtgt agatactttc aaagctaaga attgttttac
    71401 tctagctata agtcctgaaa tattcttagc cccttgttca gttgaagtac taacatttat
    71461 atgataatta ccttgcccaa tttcttgtgc tttttgtgat aattgattta actgttcaga
    71521 tgcttgttct gcacctgtag cttcaatagt aggagtggct ttttcattat taagttcatc
    71581 taacttttgt tttgtattgt caatttcatc tttaccagag ttgtttatgg ataactcaat
    71641 aaccttagtt ggatttgtat tttctataaa attggttgcc tgttcaactt cagtttctcc
    71701 ttctgtcttt atttgtacag ttggagttac tgttttatta tctaactcat ccattttttg
    71761 tttagtactg ttaagtcctt cttcattggt aacttgagga cttacaattg gaatataaga
    71821 ttcatttcca tattcatcta atattctcat gcactgaaca actttttcac tattattaac
    71881 ttgaggattt aaattaggag tgtatgtttt attcccattc ttatctaatg tttctatgca
    71941 ttcaactact tgtgattctc catttacttc tgcatttact aaaaggtcag ctttagttcc
    72001 atctgcattt tgaactgtat taacagtttt ctctgcattt tctcctcctt caacttctat
    72061 tccaagttgt ttttttattt ctggaggtaa attgtttata aattcttgta catttttagc
    72121 tttttcaagt tggtctaaat tgtttactat aaaatcaata acagcttcct ttttatcatt
    72181 tggaacatta ttaagaattt catctatctc atttagttta tctggatttt tattgattat
    72241 ttcaaatata gtttcttttt tatttggagc tttatctatt aagttttgta atgtgtctaa
    72301 tttatctata cctattactt ctgtttctat cttttttttg agattatcag gtaaaatatc
    72361 atagactttt tttgcttcag tcaatccttt tttatctaca tctatatcat attttagact
    72421 cattggagca gatatttttt ttctagcttc ctctaaagaa tttacatctt taaaagcatc
    72481 tgcacaatct gtagtaaaag acaactttgt atcttcattt ttaaatctat ccaactgttc
    72541 ttttaataca ctcaagtctt tttttgcgaa atctagtcct ttattattaa tttcagtcac
    72601 aattttatct gatttaaaag tgtctttttt gaatttttct aattctttta attctttttt
    72661 agctttttct ccattgacac taggagtgat ttcaggggca ttgtctcctg taaaagcatt
    72721 tgcaaattgc tcatttaatg cttgaaccat atttcctggt aatgcaggta acttgtcact
    72781 gatttttagt ccatttttaa gaatgtcatc tgttaattgt aatttaccct taaataagtc
    72841 atttaattgt ttaacaacat cattagataa ttgaccttcg ttttgcaacg tagttgttac
    72901 ttgtagggct aattgagcct gagcttctat ttgtgaatct tctccaaatg tgaatccatt
    72961 tattatatct tttagttgtt gtggtaaatc ttgaccagct tctccacttt gtatatcttc
    73021 aagcagtttt atatttactt taccattaac ttctaaatct tgagaaaaat catttaatgc
    73081 atcaaattgg tttttaagct ttattgcaaa actatctttg ttttcaagtt cagctaaaga
    73141 tgaaccataa cttttcaaga aagtatgtaa tttcatactg gctaagtcta atccagaaaa
    73201 accttgttgt aatccaataa gccaagtatc agcacttata cttccaccac ttagcttttg
    73261 aagttcattc gccaaccctt gtaattcttt ggtatattga ttcatatcac cggatgcctg
    73321 ataatttata ttagcatcag ttatcttctt attccactgg tctaattttt caacaggaat
    73381 tgactcactt attttatcaa aagcacttat aatgctattc tgttgtccta cactatagtc
    73441 atgccaattc atattagcta caagctcatt cattttactt ttcttttgtt cagattgttt
    73501 agcatatgtt ttagatatat cagaattagt taatctatta atcgcccttt tctgcattac
    73561 ctcttcttct tcattatatt tttcatattc agtaagaagt ttttgatgat gttctgcaaa
    73621 atctgtttct tgttgattgt aagccttttt taatgcttct gtttgttctt taaaattaga
    73681 tttacttaaa gcatttttaa ataaattatg ctcatcttca agaactttat caagtttttt
    73741 cttttcttta tcaaggtctt tttttgtatc tggtgcagtc tttgatgtag ttattgcaag
    73801 ttcatcttct tgagacttta aaagtttatt tttttcttct atagcctttc ttaatttatt
    73861 aatgacctca tctaagttac cactcatatt tagaataggg tcattattct catctacacc
    73921 caatactaat tctggtgcta tatctgcaat ttgttgttta agttcgctaa atctttcagt
    73981 ttcatcagca gtcttttcgg tcttactagc taaattattg tactcttctg atataccttc
    74041 taaactcttt ttcttttgtt gcaaggagct tatctcgctt gtagtagttc caataacttc
    74101 tttttgtttt tctgctaatt tttcatactt gtgaatagta tcatctatcc attgtatacc
    74161 tttagttata gcaaaagtag ccaaacctat catagccccg tttaacaaac ttgtcgctat
    74221 atttaatgtt ccaataccaa tagaagtcct agataaactt tgtcttaatg ctcccaaacc
    74281 tgttcctgct ctagtaatta catttgctcc attagcacca gctacatttg ctgccattac
    74341 tgcatgtcta aatgattgta gtattgtagt tgtagcttta ataggagttc ctatcatatt
    74401 taaaggattt ataagtctag ccacatttct agtaccattt gtcattccca taccagagaa
    74461 taacgttttt aaagtcataa aacttcctat taatgctggt gttgttaatt ttaatctttc
    74521 taatgttttt ataacactat ttattccatt agatacagat attaaaccat ctaaaaatcc
    74581 atatgttgta tttgaattaa atacagtttg gaatatatct gcccatattg ttttcaactc
    74641 atttaaacgc cctgcaagac tatctaaata ttgtttgttt tcagcctcag ccgaaccaaa
    74701 ttgtaaccct tgtgaaaatt cacttcttat ttctttaaaa gtctcataat tacccattaa
    74761 ggattggaat atagctgctt gttgcttacc tgctattgct tcagctaatc ctttttgttc
    74821 atcctctctg agttctcccc atttgccttt caactcatct aagagttgaa ccatattctt
    74881 caattcacca gtatctttat ttttataaat agttattcca gctatttcct ttaaaccttt
    74941 tgcggtttta tttaaagaaa gtgtaccatc atcagctgaa actttcatac ctgctaaatt
    75001 tattgcgaca ctttttaaac cattacctac tttttctgga ttttgtatcg cttcatttgt
    75061 tgctgttatc atacccactg tgtcacctaa actgacacca tactcatgaa gtacactacc
    75121 tccacgtttc cgtttatctt caattaagtt cgctacactt aatccgttct cttatgaact
    75181 gctatatgtt tccatacaga cgagactata tcttcatcct ataaggatgc tccccatttc
    75241 catatccaat agcttgatat gtacggttta taacctagtc gttgaacctt actctattga
    75301 gtcttggctg ctgattgtcc ctatctttta gattattaca cttagtacta aaagcctaac
    75361 aggagtttcc agcagttaaa ggagtttatc attatatatt actatataag gcgactaaaa
    75421 ttaatcgctt cggcaacacc tgctgttcca attgcataat tattccctgc atagtttcaa
    75481 attgttatcc tacaagcttt ttatcttgta gttcttacat ttcttattca tgtaagttca
    75541 gcatatattt tcatcttcaa gattacttgt taagatggag gacactcgtg ggaaaattat
    75601 attctatact attttaaaca taaaaatagc ataggttcat ttcctatgcg ttacggtgat
    75661 taaagtcttt taattcttta atttacctcg gtggtaacat ctcagtcttc accgattttg
    75721 ccccctcata atattagata tttctatcta acacgccaaa caatttagca tatccattgc
    75781 agcagttaat tctgttgttt tctttgttgt attcccgact tgtacttgca tttctttaag
    75841 tggttctatt ctaaatccat tgattattgt gtttactgcc gaagatgcac cttcaacatc
    75901 catatcacct acattggcaa gtatcattga ttgtcttgca gcagcaatac tttcttgcat
    75961 gcctccaatt cctgcttgta aagaagatgc tattgattct tgaacttgtg atgcactcat
    76021 tcctacatct ttagctgttt gtattgcttt ttctcttatt ctatctaact cagatgtact
    76081 atcaatatct gttggttctg caaccttttt aaggtcagtc atagagctat caagctctaa
    76141 tataacatct ttcatagacc taattccatc agtcaaagca tcgcccaaca tatctccaat
    76201 agaataagat gctacagatg aatagaaatc attaaaaaat cctccacctc ttgaaactgt
    76261 attatttaat ctttcagctt cttgtctagc atctctcatt tctcttgtta atctatcaaa
    76321 tatattgtta aaatcaccat ttagatttcc tgccgaacct tgaagttgct ctaaagaacg
    76381 tctaaaagct tcaactctct cattcccaaa tgcctgtcta atagaatctt ctattctgct
    76441 gaaattagac cttaagctat caactctttc tagatttcta acttcttgat ttatctcttg
    76501 tattctatta actatattac ctacatcaag atttaaatct atatttctac tagctgtctg
    76561 ttttaattct tgtaactcat tcctaagtct atttacttta tctgtatcta tattatcgcc
    76621 taatccatta agcctagcag atgcttgtgt tgccatatct tctattcttg aaagatttct
    76681 attcatatca gctaaatctc ttgttgctct tgaagcatca aacatatcca ttctagcttg
    76741 agatgatgaa ttcatttgtg catacactga atctaattgt ctttttaatc tttcaatttg
    76801 ttctgttgtt ctttcaattg aactatctcc tagacctgtt ctaatctgat tttgaagtct
    76861 agatacaaca tttgagattt ctttatattg tcttattaaa tcttgctgtt cattatttat
    76921 atttcctgtg tttccagagc caccaccaag taagctctct tgacttcttc tacttagttt
    76981 atttatttct ttcatagtag attcaatttg ctttaagttg ctcaaagcag tattatcaat
    77041 cttaaaggct ttattgaata tcccttttaa ttgctctgct tgttgtgata acttatctaa
    77101 ttcacctatt ttcaaattta atttaatagg tttatcttga ccactatttt ttaatttctc
    77161 tattttttct ctagcatctt tgtcatcaag ctcaatactg gttttaattt taaactcttc
    77221 aaccatttac tcaactcctt ttttgtataa aaaaaagaca gttcatatag aaactgtact
    77281 ttttctttat aaaaaatatt taagtttaaa tatttaagtt ctgacttatt cctttcaaca
    77341 tataatcata atactctatt atttcattat atttattttg taatattgtt ttttggaaat
    77401 cagatactcc gtctaaaata tttgactttt cttcttctaa tacacttttt aaatcagtaa
    77461 ttattttctt tttacttatt aagtcttctt tccaatcact tttcacaatt tcatagttat
    77521 tatatttttc agtaagtaat cttatttcat ctcctataat ttcactttga tttttagtgt
    77581 catttaaaaa ttgcaatttt ttattataaa tatcttcaga tgtactttct tcatctaact
    77641 tttcttctgt ttcttttttt actacttcat ttgtatcttc tttactatca ctacatccaa
    77701 taactccaaa tcctaacaaa ctaatcatta aaatcaataa tattttctta aacataatat
    77761 cccccctata ttctgataca ttaattatat caattctaaa ggaaaattta tactacaaaa
    77821 ggaaagtgac ctccaaatct ttattaataa gctaaacttg taaccacagt gtcttttgtt
    77881 gtcttacata tatcttctat agatttactt tctagctcag ttataccgta caaatcatat
    77941 acaaaagtat tgattgtatt ttcgcataca tttatatcgt atatgattct attaatagca
    78001 tctttgtact tattaaaata ttcctctaac tcatcaatat tcttaaaata aagcttcttt
    78061 tttttgctaa gttcattgta aaattcatca aaatctaata aataaaaatt atataattta
    78121 ttagatggat tattatctat gctcaatatc ttaaatacat ttttcaaaaa tttattagtg
    78181 tataagtata atttaccata tttatttatt agtattttac tattatttat aacaattgat
    78241 ttatcaacat tttttaaatt tggtattggt atatttttta ctgcatctgc tgtcaagttg
    78301 gtatttgatt ctccataaaa tttattataa aagtaattta ttaaactaga atttataact
    78361 cctatataaa aatatatatc ttccaaatta tcttctttaa ttaaatttat gatatttcca
    78421 ctagggaata ttatagtacc ttcaatatct actgttgcaa ttatcttatt ttgattttga
    78481 ccaactattc tttgtgttat tatcttaggt gatttgtgcc acctcatagt tttgtcatcc
    78541 atgttttcaa tatttacaaa tttatctact ttatcaatat agtatctatg tatatcctta
    78601 ccttccaaac atttatttaa aattccattt aattggttat tatcttttat atcattacta
    78661 cttaatattt tcttatcata tataattcca tcacaaattg ttttattagg gacactcatc
    78721 caacctttat gtaaatttaa ataatcccct aatttatgct tacatttgag catttttaat
    78781 attattgggt tgctttgtat aacaatttta ttatccaaat catttataat actttttata
    78841 gtaattttat tttcagagtt gtatgtatta ctattgaaaa tcttaatctt tttcatattt
    78901 tctttattta tacttataag atttatatta tttttaatat tcggcaattt tttattaatt
    78961 ataattatag cggtttcctt gtttacatcc tcaaaaggaa aaaatctata tggaaagtct
    79021 aaaatagtat caacacatac atttttaagt aaatattttc ttaaattttc aaagctttta
    79081 tttgtaaagt acgtattagg tgttataaat cccaaaaatc catcttgctt taatttattt
    79141 tcacaaaact catataaaaa caactcatac aaatccattt cgctaattag taaatcttta
    79201 tattttttcc aataatattc tctatgcttt tctggaatca ttttagttga aacatatggg
    79261 ggatttccaa ttataatatc aaaccctcct gacatcatta catcttcaaa ttcaacattc
    79321 caatcaaaag ctttagcaca atcaacctct ttgttatcaa taatagaatt tccacatttt
    79381 atattattat ctaagctagt taataaacta tacttgttag ctgttttaag ccataaagac
    79441 aatttagtta tttccacaga ttcttcattc aaatcaactc catataaatt attttttaat
    79501 atattagcat ctaagtcaaa aaatgatatc tcaccatcgg ttaaatcacg aagtatttcg
    79561 tttactgatt cagcttcttt ttttaacata gaaaaagcct cattcaaaaa agctccactt
    79621 ccacaagctg ggtctaaaat ttttatattt tttacaacgt ctctataatt tttccaaaag
    79681 tttatatatt tttgaatttt atcagtttta tattctacaa cttgtacacg cttatgctgt
    79741 atattcctac caaccacaac tgtttcataa tcttcttctt ttagttctgg aagtttatct
    79801 tcgcctaatt cttttttctt ctcatctaaa tatcttctta tggtgttatt aacaatatgt
    79861 ttcgttatat atttgggagt ataaaatatc ccatctttgt ttctcttact atcttttttc
    79921 ttaatattaa cttgatttat ttctagtttt agttcttcta agtcagatat agatttttca
    79981 aatatatgcc ctaatatatt ttcatttaaa tcactattaa aatcatataa agataacttg
    80041 tataaattct cgaaacattt atttggtatc tttagattat tcaaaatata atcttctgca
    80101 aataaacctc cattaaattg atttatgccc ttttcctgat tacctaaatc tatagattca
    80161 aacagaccct ttacttgttc ccagactcca aacctagata cacttttttt ccctacttga
    80221 atacatgttt caaagatatt ctctggtatt aatcctttat gttctgcaaa acaaacaaat
    80281 ataactctat ctaatatttt ttgtgtcttt gttaaacaag tttttacatc caaaatatct
    80341 ttattattta aaagtatatc attaaataaa tttaatctag tatttttata taggtcataa
    80401 aagccttttt ctatggcttc ctcttgcttt atattctctt cccaaagcat ctcaactcta
    80461 gattttccat gtttggtcat taaagattta gatgacaaaa gatataaaaa ttttttaaat
    80521 acatcatctt tttcatatat atctttaata gaaaaaaatt catactctgt agcatcatta
    80581 gcattataca atctaatttc cttgaagtta gagacaataa gccatgtgca atttcttcca
    80641 tatttaggca agtaagaaaa cccttgttct actggtgtat tatgattgtt ttttcttaat
    80701 tgttttgcat ctaaattagt tgttggtttt tttaattcta ttgttactct atagtcactt
    80761 tcttcattag aaaaataacc tagtacaata tcagctcttg ttccatcaat cttagttttt
    80821 tcttctggtt ttatattata atccattata ttgtccgtaa gacttttata cctaagaact
    80881 ttgtttaaaa tatccgatac aaatatttcc ttaagttgaa tttcatttaa tttatcaata
    80941 gatttattat caattatatc tctccatttt tctaaaatta atcgtctttg tttatagtca
    81001 tataagtcta ggtctatttc catattatat ttttctttta atagtttttg attaaaaaga
    81061 tacttttcca tacaattccc ctaattctat ttaaatagtt tatattttta ttatatcaag
    81121 tgtaaatggt aaaatatacc aatttacact aaaatataaa atcattcatt agataaaaat
    81181 atatattatt tgtaataaat gttagataaa aggaatttat tcttggtaac acactaataa
    81241 aataataact atctgttata ataaactttt ttattctata caaaaaagac agctatataa
    81301 gctgccttaa tatttttaac tttttcatca atatgttttt caaggtgtca gatacatctt
    81361 tatatcctat tttttctact acatctttga ctacagatta aatctcatta tctatcattt
    81421 tttataaata ggtaaccctt aaacttaagg ctagttagaa gctacagaat ttaattcaca
    81481 tgataaacca ttccagtaaa tcatctaccc tcaagtagta gctcttcaat cctatcacac
    81541 tcaaagtagt gaccactcaa acatcaaaat aagctcttat ggtggctaaa caccaaacaa
    81601 aatgttcctc tcaggaacac ttgttgaaat tccaatactt tcaaccataa aaatcaacct
    81661 cctatattcg attctaagca ataaagatat atatgtcttt caatatttcc aagtgtctta
    81721 tatttgtcat acacatgtca tcattgaaat aactacaaca taaatgattc aatttaaaaa
    81781 acaatctaaa ttgattatct ttaatattta ttaatacatc ttgatattta tattacaata
    81841 gaataaacct tctattatta taagataata gaaggtttat tctatctcca caccttaaag
    81901 gtgtactatg gccactcgcc ttatctataa tattttaata ataataaacg tcttataaat
    81961 tttacaattt aatctctaaa acctatctta taacttatac attcatcatc tacaaaataa
    82021 attatatcat tgaacataaa gaagctataa tcatttttct ttgcctgatt agccattttt
    82081 attatatctc cagtccaaat atcatcatta taattatata ttaaatgttt tggaatattg
    82141 actttattat ttgtaaaact atcttttttt aattcttact tttctattat cttaccacaa
    82201 gattccctaa aatgtatatt aggataatct aattctcttg taacttctaa tacttcctcg
    82261 atatattcaa atttaaatgt taattgaaac aactatacct ctctctttat atcaatatgt
    82321 tatatacaag tatatttcac aatatacttg tatatatttt tgcatcaatc aatgtattag
    82381 aacattgaga aacaattcta aaccacttat ctaagcaaat caactaaact acctatctag
    82441 gctaaattac tcactatatg ttagagcatc accgcttttt aattttccac ttatgatata
    82501 catctactag tggataacat acaaccctta gaaaactcta taacttaata gacactcgat
    82561 attgtctatt ttactggcac gcctaattca ttcattttag acttaaaaca ctttggtact
    82621 ctctcttgtg ccttagaatt actttcctcc attatatttg ttctaggtct aaatacagga
    82681 ttatatttag tacttccatg tccccacaca cctccatttt caaatcgctc cattggaaaa
    82741 gcatgtgctt tgctatatat atcaaaccag ctcccactat ctttaatttc tgtagctaca
    82801 gaatctgatg ttatttcaga cacatctatt gcattaagta attcataagt tctatcataa
    82861 agtgatggag cgtaatcaga ataaacttgt ttgtttactt cttcttttac tatctctttc
    82921 atttcttttc caacttcagg catagatgca gcaatcttat tttttgtata agcaaccaat
    82981 tcatctaatg atttaaatac catctaattc actacctttt ctttaatatt attcattttt
    83041 tcaatttctt cagtctcttt taaaactctg tcctgtaata tctcattctt catctgacct
    83101 agcttaattt cagtattcat taataaagat gccgttaatt cattttctat ttgacctatg
    83161 taatacatca cattttctaa ttcactagat atattgccat ctactaaatc ttctatttca
    83221 atatttaact ctatattagt caatttatca atcaacattt taagaatatc ttcttgagac
    83281 aatgctaaat caggattctc cataccaacc catattttat ttaatatctc ttctttagtc
    83341 tccttatcag gatttctaac ttctatatat tcctctttac cattctcatc tataattggt
    83401 attatagccc taacttcatc ttgtattaat ttatctactt gtattttatt catatctatc
    83461 tctcccttat tttattattg gatatttaca cttaatagaa actttacagt ttccagtaaa
    83521 cttcaatata tttacacctc ttcttaacct aatccacttt ctattaacaa tattaaacct
    83581 attctcacca gaactattta aaacagtata gtataaatta tctattgtta ttttttcatt
    83641 taattctaat ccacttataa ctaaattgtc ttcttcatta tcgctgatag tagaatttct
    83701 aatagttata tctccttctt ttagacattc aatatcaatt actggagcat attcttcatc
    83761 tacattagac acattattta gtttaatttc ccttgtatcc ttgacagtta taactttttg
    83821 aaagcttcta taagcataat ttgtatatgg ttgaaactct atttctaata cgcctttttt
    83881 atcttttgta tatcttttta ttatttttct cgctttgaga tagtatattt cttctatatt
    83941 gtcataactg ataaaaggtt taaattctct ctgccaaaac caattacata tgttttctag
    84001 cgtatagtca tcccattcgt aagcctcccc atgttcgcta cacaaacaga attgtagtgt
    84061 tataggttca atagtagttt catcctctga gtaacaagga tttttattaa aagtactatc
    84121 agatttaatc gcttcttcgt agggcaatcc atattcattt agtatttcat catcacctaa
    84181 agtaactaat ttaatgttca tggattcact tgaaatatta tcaaataaaa acttatcatc
    84241 taaaaatacc actttatcac cttctttcat ttttttaagt tcaaatccga ttaaaaccag
    84301 atttaaacgt aaaaaaataa cccctactct tttgagtagg gaaattcact atttcttaga
    84361 tttaacatcc ttttcgtcta ttaattcagc acctaacata gaagccattc taacagggct
    84421 aacatcttca tctccaacaa aagcgaatac tacatattct ttattttcat ctggcaaaac
    84481 atctagtgtc atattaaatc cagatggatt ctcagcagta agatttaatt ctatacttct
    84541 ttgtgccttt gcatttggta tagtcatata taagaccata tattcaccat cagcagtttt
    84601 agccataaca tctgcttcta ttacatagtt tggagcagta gactcttctt ttatttttat
    84661 aacttttgcc tttggtaatt cctctaagta aaacactgct actttatctc ctgcattaaa
    84721 atcagctgta gatattgtta tttctgtgtt agagcctgtt acagttgaag taaactgtaa
    84781 tttcttaact aaagatatac catctttttc aacactatat gcagatatac tattagtaac
    84841 tggttttaca ttttctaaag taactttctt agtaccatca gaagttagta attttctttt
    84901 accaacctta gccgattctt caaccatatc agaagctaat agcattgcta gttgtggcat
    84961 ttgtattacc tcttgttcaa gtgctaaact tgctgttata gctccatcaa atgctattgt
    85021 gtttgcacct ttagcttttg cgtacacgct ttctgaatct aatttgaaat taaaagcatt
    85081 taaatctgct gtataaaaaa gtggttctcc agttgtttta ttttttacta ttatattact
    85141 tgcatctttt atcgcaaatc tcattatttc atcatcctct cattttttgc ataaaaaata
    85201 acactgttat ttaacagtgc tagtttgtat ttttatttct ttagaccaat gtttaatctt
    85261 gtctgcctca acttgatatt taaaagacat tgcaaagcct aaactttcat cataattttt
    85321 aaattgtatc ttagtcttat aagcattaat taattgccaa taagtcattt ttagaataac
    85381 atctattggt acatatccat ctatgttttg aactacattt atataatcac ataagtgata
    85441 tgcttctttt tctcttttct ttctttcctt ctctttaaag tgtgcaatca actgtttttc
    85501 tctttcagag ccagtatttt caatccactt atcttcatca tctttaaata aattcttttt
    85561 atcaatacaa aacatctcta aaatcaaatc tgataccagt ccaaaatcct ctctacttat
    85621 aaaagcattt ttctttttat catcaaattt aattaaaata ccaattttaa tcttattcat
    85681 atattcaata tttttaatat cagttttata taatatagat aaagattttt ttatcttatc
    85741 actaagtaac tcaatttctt cagaagaatc ttttcttgat gttaacatca tcaaatccaa
    85801 gataggtata gcatcatatt tacatttcaa ttcaaatgtt ttctcgttac atagctgaga
    85861 atagctcttt tcaagtacta aaaatggatt aactatctct aaattagcca tatcaaattt
    85921 aataagttct tcaatagtag gttgatatat agttcctaat atttcactta gcttaattgg
    85981 taatcctgta atataataat tttccaacat tacatatcac caaaactttc agatgataca
    86041 actatgctaa atccattata acttgtatct aaattaaaca tgggggaaca gctctctaat
    86101 tttatttttc ctatacctgc tatttcctca ttttcagtga ttgtgtccac tatacaacat
    86161 actaatgcag catctcttag tccattaaaa gtatcttggc attcatcatg gcatactaca
    86221 ccaatttcaa tattagtagt ttttattatt ctacttctat ctttataaag attatattta
    86281 aataatgtta tgtaaacaga aacatctgct tcttttaata ctttttctac tcttctatta
    86341 aaaaacactt tcttgtttct aagctcttta actggatttt ttatatcagg catggataaa
    86401 atatcttttt gtgattcatt attataaatc ataaatttag cgaagtcttg atttgacatt
    86461 agagttgttc ctatattact tatcatcttg ttagggaaag ccatatagac ccatatatct
    86521 acccccttag tgttattttt ttagtatcta ttatttcatt tgtatcttta tctttagcca
    86581 atagcaaggc ttctaagcca gaatacttag ccaatgtatt agcctgtatg acacatcttt
    86641 tctcatcttg ataaattata tcacaatatc cgtattgctt gtctaagagc catttaactc
    86701 cttgaaaatg gtctattatg tactcatttt cttctcctag atttattact tcataaccta
    86761 taatcttact gaaaggaata atcacactat cattttcaga taaatcattc caagcaatat
    86821 tatttattgt atcatctttt gaaatcaatg catcttgtaa acaaatagct ttaataagac
    86881 catctgcacc cgtttctctg ccattatact caaagtcatt aatactagta actctaaata
    86941 cagttttacc agttaacatt attctagtgt caatatctat agttttagtg acaggattac
    87001 ttccaaaata aagttgtctt tttgtatctg agatagaagt atatttgtta tctgccatac
    87061 catcagaata taaggttaaa ttttcaacag ctatagggat tttatacatc tgacctttat
    87121 atttataatt aaagaattga ttgcatcttt ttattatgaa atgtttatat gtatccattt
    87181 ctttatgttc cttaaatata gttaaccaat aacaattatc atagaaaaga taacaaccca
    87241 catctacatc taaatcagat tcaactaata gatttttttc atctaaagct cttttatcat
    87301 tattagttat atcattaatt gcaactacag cttttttagt atcttcattt atacaaactt
    87361 catctatatc ggttatagga acttcaatac aggttggtgt ttcttttaaa tatttcttaa
    87421 aacttaatct tagttgtaat attctttttt ctttaggtgt tgaacaacca agtccaattc
    87481 ttttttttat atcacttaag taactcatgc tagttccatc cttcaaaatt ttttagcatg
    87541 tatctttgtt gatatttatt aaatctagtt ctcaattgtt cttctcttgc ttgaagttta
    87601 gccaacatat tagcacctga tttagcatta taatcagaat cagttagcat ctgttttaaa
    87661 ttttcttctc tatttatctt aggttgtaac caatgtattt gcataccata tgataatatt
    87721 ttaatttcct ctaaatctaa atcagatact atttctccta aatatttgta tttgaaaatt
    87781 atctcttctg tagtttctac aacaaaactt attataaatt tttcatcttc aaagcttatt
    87841 gtataatctt tatctaattc gtattcttct tttgtctctt ttcccatcaa agttatctct
    87901 gcatttttat tactataatt agatgtataa cttagttgag cagcaggaat aatttcctcg
    87961 cctttttcat caacatattt tatagtcaaa tcttttttac attgattaaa atcaactata
    88021 gcatcttcta aataactgta catcattttt tctattattt catttgatag taataacatc
    88081 tcttcatcat taatagcatc caaaaacttt ttatatattt ttacaagtgg agtacccaaa
    88141 tattccacca cctttaacta aacaagtaca aatcttctat tccaattctt ttagctaata
    88201 gtttagcctt tagcccagaa tcaaactttt tctttttatg taaatctatt gccctctcag
    88261 ctaatcttct tgttaactca acattatttt tatttaatat tttttcaaaa gaatttatat
    88321 caactttatc agataagaaa tattctaaat agtcttcatt aggatttctt atatgttcat
    88381 ataaactatt caatcctaga aaatctatta tgtccattgg ggtacattca acatctaaag
    88441 aatctacatc cgaaattatt aatctatgtt cttcaaaaaa acctggactt cttctcacta
    88501 ttctataaag agtatctaaa tctataaatt cagttgaatc ttttgaacct actttagata
    88561 aagaaaaaat ttgttgtttt tctcttgggt caatataatc aacatcccat gtggatatat
    88621 tttttatttc tatatctatt tcctgttttt tttgttttag ctttctttgt agttctaatc
    88681 ggtttaatct agcttctttt ttatttaaag tagactttat atctttttct aaagtattag
    88741 ctttgacttc tgttttagaa atattttctt ttttagctct tgccataata atctccctcc
    88801 cataaaaaaa gaaggtggtt atccaccttc taattcaatt attgtatttt ataaactcca
    88861 aatttagcag cagttacaac tcctaaatgt attttttgag ccataaaata ttctatttgt
    88921 tggtcatctc tttctttttc atcagtattt tctatgacca atacatctcc ttcagtacca
    88981 agtttgatta tcttctctcc atcaggtatt acatagatag ccttattatc taaagcccac
    89041 tctttacctt ctgctgcatt ttcatcataa gcatttggta aactaagtac tttatatcca
    89101 cctctaaatg tttgtataaa accatatttt cttttttctt ctttttctaa atcagtttga
    89161 ataccttcta aattacttaa tgcaagtgga gtaccatata taataggctt tttaccagta
    89221 gccccttcta ccttagctat caattccaat aatactttgt ctgaattacc tgtagcttga
    89281 aacttagcat ttattgttga atatgcacct tcaaatgttt ttgctattct tctagcaata
    89341 tcataatgat atgaatctga taccctattc acaaatacaa cccaatttat tcttcctgtt
    89401 ataaatctct ctgcttcttc atatatagca acagataatt taaatgctgt tgtaggaact
    89461 tttttgttaa gtaatctttg tcttcttgta ctattaacac catctgctat atttgcaact
    89521 ttaaataaat tagtattttc aacagtaaat tcttttttat ctcctaaatc aaagttctca
    89581 aaatcacaaa atggactaaa tacttcctca tttaatctat taatatcatc agatattaat
    89641 tctgatatta aattataaaa tccccattta ttttcttgga atttataata attatatttt
    89701 tctcctccca tcatttcttt taatttattc tttattgcac tttcagcatc tctatatgtt
    89761 attgtatttc catctctatc tgtatattct ctaaatgtat ctgtatatag ctcatgagct
    89821 attctcttta aagtttttgc tgttgccatt aatttacaca cttccttttc tatttttttg
    89881 cataaaaaaa taacagttat agactgttat ctcctgtttg ttaattttat ttgattttat
    89941 aaaactttta ttgtaaccga atcttgacct tcatagttcc ataattctaa tacttctgct
    90001 actggagttt tagcatctgc tgtaggcaat ttttcaaact gaaacgaatc agattttaat
    90061 tgtaacttgt cttttactgc aatactttta tctgctatat gtaatagaga tatagttacc
    90121 tcatctcctt tttttagtcg tctacatcta catatctgac caggagaaac ttcgtaatct
    90181 ctttcatcta atctttcatc atacatagtt gctgggtcat caactatacc ccaatctaat
    90241 gtgtcatttg taactttata tattttatgg gtatccctac catactccgt gtcttcaagt
    90301 tctccaatat ttattacagc accattttct aaatttattg gattggtatc ctctgatgga
    90361 acaacatatt tacctgttaa aacatcagga ttttttactt tatctgtttg tattattgct
    90421 ttatctttct tcatgtaaat tcctcctatt attctttatc tgaatatttt tttataaaag
    90481 atatttcttc tggttcacta aaactatcat tattattact tataggtatt tttatcgtat
    90541 tatttttatc atcatttata ctaaaatttt ccttttcgct ttgtttattg ctaagagttt
    90601 ttaatgcaaa tatgtaaccc aattctttct tgtattcatc taatgtaatt tccttattat
    90661 aagcttttat ttttatatct ttaatttcat cttctgctaa tttaaatttt tgagctacat
    90721 catcaacttt agatttaaat aactcaaatt ctttttcttc tttgaatgcc tgtaatggct
    90781 ctaaactggc ttttaaatta gatatttctt tttgcttttc agtaaatttc tctattattt
    90841 ctttttttaa tttttcttgt ctactaaaaa cctgtactat ttctccttct tgtttttccc
    90901 tccaagtttg tatatatgat tttttatttt cataatccaa aatgacagca tcttcattaa
    90961 ctatatatgg aataccatag taataccccc agttaatttc atcttcaact atagcaatat
    91021 tctcttctgg tattaaatca ttataataaa aagcttgtac ttcaactttt tcaccatacc
    91081 aattctcagt ttcgactttg tattctttta acttatttct tatttcagta ctaagattac
    91141 tcactgctaa agcatatcct tcagaatttt tattaagttt ttgattctta tttggcaaat
    91201 tcttttcccc ctctccatgt tctaatgcat aaatttctga gcatattttt gatatttctt
    91261 ctttataatt aatttttctt gaaaatttag ttaaacaaca atttgaaccc atagctccct
    91321 ttacatcaga accaagacat gtaattccat gatatacata gtcttttata ttataaaatc
    91381 catctgaatc atctaattga taatcattta tatctatttc cattgaaatt tctatttcat
    91441 cagaatccat aattatatcg taagcatgat tgctatatga tttatatata tatcctttac
    91501 atttaacata cttctgccca tcaatttcaa ctttttcata tttatttgtt tctggtataa
    91561 cgccaattat tcgttcttca taatattctt ctaatataaa cccttcatct gtttgaacta
    91621 cttgtgttaa aacattatga ccatcaaagt catattttcc atcttcattt atttttacat
    91681 atcctagaat tggaacattt tttaatgttg gttcagcttt atcaatagct tcttcataaa
    91741 atttagttcc atttggattt gtaccttcat gcataacata gatatacact ggcaaaagat
    91801 tactatttag ttcttccaat ttttttttat ctgaataatg ttttaaatta caaggtaatt
    91861 ttaatatttt tatttttttc acctcctttt aaaaacgtat tttgctagta aatataaatc
    91921 ttttcatttc ttcattactg aaattattta ttaattttga tttattttca aaaacatata
    91981 aagtcttatt attcaacttg ttttcacata taaaacgata tcctttttcc attaataatt
    92041 tcttatcatc ttcagaaaaa gcatatataa acttactcat aattaatcat tctccccatt
    92101 tccttttgct tgagtagaat taccattatc tgtccctata tcagatttat ctggtctacc
    92161 actgctttga gtatgagatg tttgttgagg tattaaaaac tcatcaaatc ccattaattt
    92221 ttctgcttgt aaagtgttta tagcttgaag tggagtaaac ccagtacttg caataaacaa
    92281 gcttttactc cctccgtatc ccatatcttc tcgatactgt ttggataaat tcattttatt
    92341 aaagtgagta gttccaataa acttcatatt aaataaattt gccttcttgt ctttatttag
    92401 ttcaaagtta acccaatttt ctatcatttg ttgaatcctc ataggaatta agctatctgc
    92461 aataatacca tttaagatag attcattatt tatcttgtca gagttaagta aggctgtatt
    92521 tattccagcg ttatcaaata taaactcttt tgcttctttt acataatcat ttattttaga
    92581 ttttccatct gaaagagtat gtgtttccaa ctctaaagga tttgtagtta ttgctgttcc
    92641 ttttggtaaa tttgcttttg ttgaattgtg gtatatggga attaaattaa ggtctactag
    92701 tggttcatca tttttgccaa atggtatctt ttgatgtatt aatttaatac tttctattac
    92761 tgcatttgtg ctttttaaat ctttcatatc ttcaagctcc attaaatcgt caaatataaa
    92821 gcaaaaaaat ggaactcctt tcactgaatc taaatcataa ttaaatgcta ctgctttatc
    92881 acttaattga aaatatgtat tatcaattaa ttcttctcgt gtaagtaaat tagatttata
    92941 ttttttatat atgtttttaa tttcttctgg aaaggatatt atgtttttgt cagtaagttt
    93001 ttttatattt atagcgtatc tcattacatt attttctatc attgttatca tacaaaattt
    93061 tgctggtatc tgttgtaaaa ccatccctga attatcttct atcttatata aataaacctc
    93121 tcctaattcc aatacttttt cggttatcca agctgatgta aattttatat tatatttttc
    93181 taattgactc gccgaattca tatatgcttt tgttattttt tcttttgttt ttaatctact
    93241 tatgtctttt gggtataaga tatggtcaaa agtattcata ttagatatta aatttaaaac
    93301 tcttctatat gtaccagatg tagcttttaa tatcatactt gcatcctgaa gtatatctgt
    93361 atttgaaatt tgatatggat tggagagagc ttttcttaat ttgtctctac ttatacttct
    93421 taacttatac atttctgttg ctaatgttgt ggattctttt actgaggcat attttccaat
    93481 ttgagcattt agcatattga tttgattttg tgtagaattg ttgtctttag acttgctttt
    93541 attattattt tttttgccca cttattcacc tcatctctta ccaataaatc ataacagttt
    93601 tttgattatt tctatttttt cttttctctt tttcttccag ataatctgct aagaaattcg
    93661 tataagccaa actagaatat ctatcttttc ttgctccact tttttccttt attttaattt
    93721 ttcctccatc agcactatat tctaaattta ttaactcatt cactaaagcc gttgtttgaa
    93781 gataaggggc aatatatttt gcttctaatt ctgccttttt atctatttca tctgccttta
    93841 tcatttcttt ttctaatatc atttcttttg cttcaatatc acttatagga agttctaagg
    93901 ttctatttgt aaggacatct cttaaagaca tagccatttt atgattttca tcagcgtaag
    93961 ctttcatgga atatacaaca ggaagtgatt tttttgctag tgacttgtca acagtattat
    94021 cttcattaaa acatgtgtaa gcatcatacc attcatctct atctgaatca taatttgctt
    94081 tctgtatata gctccataca gttgttccta taccttgtgt atctaatatc atataatctg
    94141 cctgaaaatc tgaaaataat tgttttaatc ttattgcttg tttttcagct tccataccat
    94201 tataagcttc tatatggaca acacatcttt tatatgtgcc ttcatttgga agcatcctca
    94261 tcaaagtata aatagaatta tcattcttaa ctcctctggc taaagctata tctgcaccaa
    94321 taatccgtat ttctcctttt atcttattaa gatgatattt cttatctctt tttttcttat
    94381 tttcaagata ctctatatca ctaggaggat aaaacatact ttctaatacc ctacatggat
    94441 tcacccaaga tgacttgaag aaagcatcat ctgattcatt ataaaacact ccacaatact
    94501 ccatcatgaa agaagcatca ctcatactct cttttttcat ttctttaagt attctttttt
    94561 tagttaatag tccatgttct aaagagcaag tgaatggaat actacatgca aacatatctt
    94621 caccactaag catacctttt actgtttcca taaacttttt ataactccaa tgctgagaat
    94681 accagcctga actaagataa atttctttat tctcttcttg catatatcta tatttaggat
    94741 tctgtaaata tcctggttgt cttgggtttg ttaaaaatgg aactaaaaca tcattcacag
    94801 ttccttcatc caccattcta tattcatcaa caataagaac attagctctg taacctcttg
    94861 catcatcatt agatacaaca gcttctattc tacttccatt ccaaaaatct atagatactt
    94921 cttgtgcatt tacttggaaa cttttaattt ctcttttcag agtatctgat tttaaatata
    94981 aatcacctaa aattttactt gtaataagta gttttgcttg tttctttctt tttgctgcta
    95041 ttaaaacaca agtacctggt tttaatacag ctataacaca acaaaacact cctagaagcc
    95101 atgattttcc cagtccacga gaagcaatga atacaaattg gtcagacttc atcatcatat
    95161 aaattaatat ctgttgaaat aaatgtagat taaacccaaa gtaatccata cagaatctat
    95221 gtggatttgc gagataaaaa gcaccccaat actctcctat actctctagt agatttcggg
    95281 atgaatcatt agatgacagc tctctttttt catatttagt cctcattttt attaccttct
    95341 aaaatgtctc ttacatcatc tttaaattta atatctttat cgccatcatt tatcgaatac
    95401 ttacctgtag ccaaacctaa tgcaacagca agtggttttt tcatatgttt atttacataa
    95461 gcttcaaatc catctacatc ttcatattcc tttaacctat cctttacagg tttatttttt
    95521 tcatatatca tcattttcat tccaaatgta tcatcttgcg aatctccagc tttctttttc
    95581 tgactaggct ttatttcagc atcttgcatt ttacttgata ctaatttaga taaatcatta
    95641 aaagctttca tatctccctt ttctcttgat ttatcttttt ctaattcaat aatacaaatc
    95701 tcttcaaaca attttctttc agttaatgac tctggttcat aatattcttt atattcctta
    95761 tattttcttt ctaagatttt atactcataa tcatcaaaac ctcttcccca ttttaattta
    95821 atttcatcat ttatgactat atttttatca ttattatttc ctgtatatat ttctttcaat
    95881 aaattatcta aagatgtctt tcccttatat tttgaatttc tattaataat tttcatatat
    95941 tcatctatta attcatcttt atttttttta tctgcaagtt ctttagctaa tttttcatca
    96001 aaataaacat caaagttgaa acatatatgt tttagtgcct ttacaagttc tccattgtaa
    96061 cacccattta atagtaaaaa cctagctcta aaacattctt tgcatactgg aagtctttta
    96121 tttaaagtaa atatttcact atgacttttc caaaaatatc tatctgtata tttttcttca
    96181 ccacaattca aacattttgt tttactaatt gccattcata acatcaccac cattaaaaaa
    96241 gaccactaga gatttaattc tctagcggtt aatagggaga ttcaactcaa ttaagagctg
    96301 agttaaattt acaatataaa cttaactcag ctcttaataa ataacttata gcttacttaa
    96361 atttatattg taagtacata ttctcccact ttcattaaat atcattaatt tttgcattgc
    96421 gtgtgaactt aatcttagat tcttagcata agaatccgtt ccggagaaac ttccatttac
    96481 acatatttca ctttctccat aattatcttc cttacaattg tgtagatgag ccataaagat
    96541 ataatcagga attaatttta taagagaggt cagtttagga attgctgtag acattttatc
    96601 tttatctcca tgtactgcga aacatgtatt tccacatatt ttagctacta ttatgtcatt
    96661 atcatatata ttttcttgga atattacatt gcctaaactt ttaattctta gctttatata
    96721 ttcatctatt aatagtgtaa aattatcttt atctaaattt tcatcttttt taggaagaac
    96781 tctatcatga tttccaccaa ctgaataaac aataatttta tcaatttctt tagataattc
    96841 atatataaat tcactaagta tttcagatac ttctataatt tgttcaacta tattttctct
    96901 attttctaat cttatagaat tatgtatgtg gccagaaatt aaatctccaa gtaacattac
    96961 atgcagtctc ttaactttat gtaacttact atattcaata attttatctt ttaaatattg
    97021 tattcttatc ttgaatattt ctgaattata tctattaaat gcattaatag tctctaatcc
    97081 ataatgtatg tctgaaatta gcataatagc ctcttgattt gaagattcat agcatttgta
    97141 actagaatca cttagaaatg gcttatgaag tgatatatca tctattttat tattaaataa
    97201 atcaattata ttatctattc ttgaatattc tcttattttc ttatttacta atgacctttc
    97261 atcagataac tgtactttta tctttttcag ctctaatatt ttctcattta ttttatcaat
    97321 ctcttcttga gtagaattat cttgtacttt agaattaaga taatcaaaag cctctttcaa
    97381 cccataagaa accttcctac aatggtctgg agaacattct aaccctaata gttcaactat
    97441 ctctccccaa tccaaatcta tctctttatt aacctttgcc agtcgtattc taatcctata
    97501 gtcaattagt gactcatctt cttttttctt caatatgtct tccatattcg atatctccct
    97561 atttatcaca taaatatcat tatcaatgta ttaagtaaaa aataaatagt ccaatattga
    97621 tttatttaac tattgtttca cttaatctta tagttatatt taaatctgtt tttccaatat
    97681 actggtcaaa aacatcacta gataacactt cattatcgcc attttttgta tgctctataa
    97741 atttataagt accatcttct tgttttatta cttcacattt ttctaaaatc gttcctttat
    97801 tatttttcat atattttctc ctttattttt atgttatcta tagctctact gggaatatat
    97861 ttatttgcaa attaggcagc cttgtagtta acaatagcta atataagtta acgcatttag
    97921 catttatcaa ggattttcta taactttttt attttatttt tactatttta ataatttaat
    97981 cttttatcct tgagatttta tatttgaatt tttaacttta agctttatat ctattgttct
    98041 cttttataca gtacaatcgt ttcgtttgta tgtacccatc acttttatat aagtagtttg
    98101 atttttaacc caaaatcaac tgggaactta taatttatgt tattcgccta acctgcaaat
    98161 taaaaaatag cccaattaag gactatttta aatctacatc tataattgta agtgcatttg
    98221 atgtactcaa tgcaaaatca acttcttttt caaattcttc tatttcatcc cttaattttt
    98281 ctattttctc tcttactttt aaagggtcta ctaaagacca ttcttgttgt tttttataaa
    98341 atgaaataag ttctgctcca tctttacttt tttctttttc ctcaaacatt ctgtctgtat
    98401 ttttttgcac ttcttcattt ctacgattta cttcagacat catacaatca tagttactaa
    98461 ccatttgtct caacaaggat ttatctaatt ctatactatt ttttcttttt attgcttcta
    98521 aaactgtata atttttatta ccaatttgaa catttgttat agcattagag atattaattg
    98581 ctttatctaa tgcgttataa ttatatatta aatcttctat ttgttggtat ctacttgaaa
    98641 cttctacatt aaaatcttct acacttgttt ttgtttcata tacattttta tcagagcctt
    98701 ttttagtacc tacaatttta aaattactaa tgcatctatc tatcttatct tctaataact
    98761 tcttttctgc taaagcttta tgaacattat atttaaccat aaaaacatct cccttttaat
    98821 taagatatta tttaaaaata acaaactttt tttattggaa gagaggaagg gatttgaacc
    98881 ctcgtatcaa attaattgac ctaatagttt agcaaactat cctctttacc acttgagtac
    98941 ctctccatat ttctataaat tacaaacagc aagtcgagta tcgctacctt cgaccatggt
    99001 caagtgctgt attttaaaac tccgacagct cggatagcgt ggctgttttt tcacactaaa
    99061 gaatcttttt taattgtcca ctatttggta gaacatcacc acttttttat aaataaatcc
    99121 acataaaaat tacttagttc aaaagactat ttgatttatt cacaatttct tcagtttttt
    99181 attagccagg tacaccaaca aactcctcta attttaaaaa gcagaagtag aatctgttac
    99241 ctcaaattct acttctttat tcttacacct ttaaggcata ctagggcatt caccgtatct
    99301 atttcaagtt atatgttgct catcaacctc gtgtatattt gccacgctat attcatttta
    99361 gcagcatcat catcttatat ttgtaaaata cctattacca gccactagag cttctcaaaa
    99421 tgagctatct ctgctaattg tattaatttt tagcatattc taggtttgct gccatatgtc
    99481 accatatgaa agggatttta acccatacac tctctatatg attgcatctt ttaaagattt
    99541 agctattttt aattttggaa ctcttttttc ctcagttttc caaggttttt tagttccatc
    99601 tttaagagtt atttctcctt ctttaccttt ttgtatttta gttaggtatt taactcccat
    99661 caacgtcaat tcttcaccat cttgtagtac ttcgtttctt tttttttcta tcacatcaag
    99721 tatctctctt actcttatct gtgatacacc taattcttct gctacttctt ttaaaaaatc
    99781 tacttttttc atatcttctt aatctccctt ttcttatata attttcattt gataatagta
    99841 aggaataatt ttattatttt tattaacccc ttacataagt aatatgtttt aagcgaccct
    99901 tttttaaacc acttttcccc accagctaat tacagttttt acaatttgag cgatatccct
    99961 ttttaccatt tttatcaaac ctagatagca acttaatttt tccacatctg ctacattttt
   100021 tatattttcc tttacaaaca gatatataat aataattttc ttcatagttt ttctcatatg
   100081 catctattat tttgttcact attttattta tttgattatt tattgcttga ggagttatat
   100141 tcattaaatt tgaaataaac tctaatgatt tatcttttct atataaattt aaaatatctt
   100201 tttgtactgt ggtaaactca attttagata taatattatt taaatccata actatacatg
   100261 ttaaatcatc ttgcaaatca ttaccctttt gaatctttaa aagctcttta acatgtgatt
   100321 tatcaaacat atctaaatag tcccattctg ggcttcctgc atctgctaat ggtgctttcc
   100381 atataattgg tctctctata gaatttttaa catcagtcat atcatttttt aatgcattta
   100441 aatttttcct aaccattctc ttaagtttta actcttcttt acttaattgt ttatgtccta
   100501 attctttata tttagcttta taccattcat aagtatctga gtatgttttt ataattggat
   100561 attttttaat atctttaggt ttaactttta tatcttttat ttttttatag tttttaggta
   100621 attgaaaaaa tgcaaaggaa ttgtcttcta ggaatccatc tttattagaa gtgtttgaat
   100681 ttatatttcc accatttgca ggtgctattt tatttagaag tgcttcttcc tgacatgctc
   100741 tatcaaataa ctcttttgaa ttgtatattc ttattttagt ttccttaggt ttatcatcta
   100801 ctgagagtat atagctcccc attatttcta atgtttttgc tatattagaa gtagaatata
   100861 tactgtcatc tttatttaat atcaacttaa ttttcgatgt attatgatgt gtgtactccc
   100921 atgtaccatt actattacta aaaggatttt taacatttat atattcgatt ggattaattt
   100981 gattatagcc acaccaagta atgaatctat tataatccat aactacttct tctccatcaa
   101041 tagtaacata atatacacca tctttatcta aaggactatc tgatgtttgc tcaaatatat
   101101 tttcccaaaa tttatcattg aattgttttt tttcttgata tttcactcta gtaataatat
   101161 tctcatttct ctttgtaaga gtattataaa atgtatctat ataatttcta tttgactctt
   101221 caaaccacgt gtgttttacc cctaatcgtt cctctatgta tttaattcta tctttgctat
   101281 tgtctatgct ataatctaaa ttctttatgt aatcttgtgc agatttgcca tctatttctt
   101341 cataaaaatt gttattatta tatatcaata ttatacctcc tttccttgca atccatcatc
   101401 ataatctgta tcatcataat cccatagttc atccttaaaa tcctccttat gcctttcatt
   101461 tacaatatca tatgaagacc ttttactcat ctctgtgtat gtatctatca tttgttcata
   101521 ttcctcattt aatatttgat acctattttt atgtacgcct gttttctctg attcatctaa
   101581 atcttctaaa tatattaatt ctattttttt ctctttcata cttctccttt tctatattat
   101641 aagattttaa attaattttc ttggtcacat ttctgtgccg tttttttgcc ttttggtcac
   101701 atttctgtgc cacccctaaa tttaattttt taactgtttt tcctatattt ttgttaattt
   101761 ttacagttca ttacctctta ttcttagaca gactttctaa atacaattaa aaaagcataa
   101821 atcaattgtt ttctttgcta aatttaaatt atttcccttc catattacac aaggatttat
   101881 aatgaaatag tctgtatttt tgttattccc ttcaacgatt acatatttaa aaaaataata
   101941 tttatcatct tctaccttta tataaaatct aagtaattcc tttttaaatt tgttcatatc
   102001 ttgttttatt gtactaatat ttaataattg acaaatttta tataaactaa ttttttttat
   102061 ttttgaaata tctgtttcat gagggtttaa acataagata ttactctcga aatgtatata
   102121 tggtatgagt tgatatatat aacttaattg tttatgttgt cttggtgtgc aatgttcaaa
   102181 aagaaatcta ctagtattta tatatattct tgtatattct ttattttcaa aattaggaat
   102241 tcctttagta aaatactttg gatttatata aaatttatta ttagattcat ataataactc
   102301 acattttttt acgtctgcta aaaaatttct aaatgcccta tctttaagat ttaatatttc
   102361 tctcatgttt tttctatcta taggaatcat ttctttaaac tgagaatatc taaccaataa
   102421 attttcttct cctgtattat agtttagata tgttgcgaga tatattaatc tacttatatt
   102481 agctttactt atatttactt cattaaataa taattcattt ttaacataag tacaaaatac
   102541 aaaacctcct aatttaaaat tatggtatgg taattctttt ttattattga taatagccct
   102601 ttgctctgga gaaaaactct tttttctctt tccaatttca agtacctcac ctcttttcat
   102661 ccctttatgc tccaatattt ctcctgttct tttgtctgtt acaataacat cttttataaa
   102721 ttcattacct ctaactataa atatctacct cttttcttat aaattttatt tcaattacaa
   102781 gataaaaacc atcttgcaat tgttcttcgc aacatgttgc ttcagaaatt ttattaccct
   102841 ctatgttcta tttctattat atcatatacc ctctatgttc tcaatatttt ttttaatatc
   102901 tacccaaaat ttcttgagta gatattttct tttaatctgg catattatta aatatattct
   102961 taataatagg attttctctt tttatatctt ttttttcttc agtaacattt gtctttttat
   103021 ttgtcctata tacaccattg cctaaacatt gttttaataa tttattttct tcttgaagat
   103081 ttttccactc taatattatt ctttccaaag ctgtatttct actgcttata tttttatcat
   103141 tttgatactt ttctatttca ccaaatatat cttcttctaa ataaaagcta gatgattttt
   103201 tcatataata tcactcctag aattgttcca tacctacttc aaataatcct ttagctgtag
   103261 ccatttgtgc atctttagaa actttaaatc tattatcgaa tgtcatatgt aatgctgtac
   103321 ctccacttac atatacatcc atttcatcta tgttcttcca tttagaagat attctttgag
   103381 ctgtgctttc agaagccata atgtaagcaa ctttcttaag ttcatcataa tcattagaac
   103441 tatctatctc attaagctct cttattattc ctctatcttt tagttgcttc tgaaccattg
   103501 ttaaagcatc ttgatttcca gcttctaatg tgtctgatag tttatcatta aatacaaaac
   103561 ctttttcaaa gaaagacatc tcagtacttc taaaacctat tgaaaccatg ccaaccgctt
   103621 tatcttcatt tacttttcct tctaatgtat gaaataatgc agcgtctcct tctctaaaaa
   103681 ttgatatatc agatattttt atttctttat gtccaccttt tattttatct tttactttaa
   103741 atgttttacc tttatatgtg tctacaactt cttttaatgt tgttttccta aatgatttat
   103801 atggaacgcc catcattaat tttacttcat cagctacagc tatttcatta agagcagcag
   103861 caactaatat tctcattgta aaagatactt tagaatcatc agaatttctt ataggtgtta
   103921 tactttcttt ttctgctaat gaaccaacaa agtaacttac actttcaaaa tctatgtata
   103981 ttggattttc atatttctca aaatctatgt ttcttccatc accaaatact gatttgaata
   104041 aacattcttt aaccattcca ttgtacttac tatatccttt tacgtatcct cttcctatat
   104101 ctaagcctat aacttggtaa ttttcttcca ttttcattcc tcctaagtaa atatttgcta
   104161 aatatattat accatataaa aacttgataa ttacattaaa tttgcacaaa aattcaacta
   104221 taaaatgaca ttttttagtt attagttaaa attagcacta aattagaact atatataatt
   104281 taaatttaaa ccaataattt atcattaaaa atttttagtt aatatttaaa ttatatatca
   104341 actatttcca aaccaataaa tcaatttctt taactacact atttatgttt ttttagatgt
   104401 caattttaat tttaagtaaa atattgaaac ttattttctt acagacgaca ttttcattct
   104461 aatttaataa atcttcctat atttcaagtt attttttgta tttaatattt ttatatgtat
   104521 tagttatttt tatgtaatca atacatacac ttttatttta tattttcctt gttgagaatg
   104581 taatttaaga gtaaaaatct aagaaatatc tttagataaa tatttgtttt atgaggaaaa
   104641 tacagagcta tttcgtctgt aaatccgtct gcaatttttt tgaaatgtgg aaatgaaagg
   104701 actacacaca aaatcctact gacaaacaag ccactttcca taaaataccc cctacttcat
   104761 ttttatttat tttaatatgt aaaatacttt atttacttta tttaattaat aaaagtatta
   104821 cttttatctt attttaaaaa tctatgtttt tgtcagtaaa aaaggacata aaaattagta
   104881 ttttatgcca tttttctaaa acctagtatt ttcaacactt acaacaccta cactttctat
   104941 tttgtcagta aaatgtcagt cattataatt cttagactac aaacctgttg taatcactgc
   105001 atttcaattt ttagttattg atatctatat aaaatattat ttgctataga ataaattttt
   105061 ttattatgga ttataaagtc ttatatacat cattttagca ttaatctgac atcgtcgaat
   105121 atcaactcac aacctcattg ccatattttt cttgacaaat ttgtcattat tgtatataat
   105181 ttaattaaga taagaattaa tcttatctat acaattcaat attagggggt gacgttatga
   105241 gaataacaaa aggcaaaata aaagagctaa tcgagttgtg ccaccaactt gaaaagctct
   105301 ttgtacaagt atttaaatca cttgaacata ttttgatttc tttatgttct tgtatcggtt
   105361 ggttattaat acttatatac ataattaaac attaataacc aatgcaatat acctataaaa
   105421 atatatctct ataggtatat tgtaacacac ctaatattga aagtaaacga ggtgacgaca
   105481 tgagtcaaca tattaaaaac actttaaaaa taataaagat actaggttta ttattcttct
   105541 tatattttgc acttagagca atatatattg tttatcttta atatctttat tgagttgctt
   105601 aatgtataag taactctttt ttgttataat attaatgagg tgatagcatg aaattagaaa
   105661 aattaattga tataagaaaa gaaaaaaaat attctcaaga ggatattgct agaatgttag
   105721 acatatcttt aagaaattat cacagaaaag aaaaaggaga gaatcaattt acagtaagtg
   105781 aactagaaaa aatatgtagc tatttagata tagaatcaaa agaactactt gacatatagt
   105841 aatataatct actcaaggta gtacttaaca ttaaataaaa taatttaata atataaaata
   105901 gactagctat aatctagtct attttattat aataacatat tgtacaagcc atttaaatcc
   105961 attctaagca catttcaatg cattcacctt atgtttatac ctttaatctt taaaattaaa
   106021 tataaaaata agctagacat atatactcta gcttacagta atttttttaa tgtgttgact
   106081 tgttcttgta ctattgtttt actcttagtg tctaactctc tataattatt taatagttgt
   106141 ttttcttctt cttctagttg tatattttct ctattgtctg taagacataa tatataatct
   106201 atacttacat tatatatatt agataatttt ataagcgtct cattgtcaat ttttcttctg
   106261 tttgtttcat agttttgtat tgttacataa tgtacgccta atagttctcc cacttgttga
   106321 gttgaatatc ctttttcttt tcttagctct ctcaatctat tcattaaatc acctcttaat
   106381 tctaaatata acatattata aatttaatta caattgtatt caaaacggta tgttttatat
   106441 atgacattat ttaaaacatt ttgttttgtt ttttatattt tatattgaca atcaaaacat
   106501 tttgttttat aattaaatca tagcaaggga gaacaagaaa acacaaagcc acctctcaaa
   106561 tgaagtaaca ggcaaagaac cttcttaaaa ctctagctaa tatcttaaag ggttctatta
   106621 aaaaatatta aactactatt aaaaaattac tacctttaag ttgacctttt aaatggtgtg
   106681 aatggtgaaa ggcacacaac acaaatatta cacatagttt tttcttcatt ttttaactac
   106741 aaaaatgaat tagtcatacc agttggtgag gtgttcccaa cattaagtaa ttaaataaat
   106801 aaaattaata aaggtttcca attaaagcct acaaaaaatt gaggtatcaa cttagttgct
   106861 tctataatgg cactcaatac aatttaatat gacatagcag ttgtgaggta ctgcgttata
   106921 aaaagtacta aagattgagc attaaaatta tagtttgtac tgcttgacac ctacatggtt
   106981 ataaactatc taatatttac tagtataatt taaactattt attagattat tttagtaagt
   107041 gttagaatac aacacttaaa ttatgatata ataataaaat aggagtgtga acggcatgga
   107101 tgaaaaaata ttagaattat tacaaaaaat ggatactagt attaatgata tacaaggaga
   107161 aataaaatgg attaatacta gactagatag tatggatggt cgttttgatg ggattgaaaa
   107221 ccaacttagt gatttaaaag aaggacaaga agaaattaaa aagaaactag acttaactta
   107281 taatcaagtt gctagaaata tggaaggcat aacagaagtt ggagaaaaaa tagacacttt
   107341 aaaaaatgac atgaactttg tagaaatggc tacatctaaa aattggaatg aaatagcaaa
   107401 gttaaaatca attaaatagg agtagataaa atggatgaga aaacattaga attgctacga
   107461 gaaatacagg aaagtattaa aattttaaat gacaagcttg acgaatttga ttatacacaa
   107521 gataaaataa aatctaatgt tgagggactt ttagaatgtt ttagtcgtat tgattttaga
   107581 attgaagacc tagaaaatgg tcaaacctca ttgtatacta agcttgatat agtgcaaaat
   107641 gaaacagcaa aatcaattaa atagcagtac ataaaaagac acttgaatta ttgagtgtct
   107701 tttctacaag ctcttagtat ggtattatta aacaataata tcattggagg tataacgaat
   107761 gtatgagcag agaaaagcaa aagtaatatt caataaagga gctggtaatt ctaggggaga
   107821 atcatatacg aatagggtta caattccaac tacatggata aaacatatgg acataactaa
   107881 actagacagg gaagttttgt tgacttttga tggtaataaa atagtaatag aaaaaataga
   107941 tgagtagata aaaaatctac tcaaaaaaat acttgacaac tcgtgacgag tcgattgtaa
   108001 ttaaatcata aaataaaatt ttttacaaaa tgactagtaa cgagtcggta aaaagggagg
   108061 atataaaatg ctaaatatat acataactaa tttagggaaa tatgattgag atgcctttta
   108121 tatagttctc taatatggta ttattattct ataataataa tgttggaggg gacaaaatga
   108181 atttagagag actaaaagag ttaaggttag aaagaggata cagtcaacaa caatttgcaa
   108241 cttatattgg attgtctact tctacatata cagataaaga agcagggaaa acaaaattca
   108301 aggttgaaga attagagaaa atattaaaat ttctaaatct tgaccctttg gaattgcttg
   108361 acttagatta aaaagttaac ttttctaaat aataaaaccg aaaaatcgtt gacaatatcc
   108421 gttttgagat ttataattaa aatatcaaat aaaaaattta aaaaacaaaa ccgaaaaatc
   108481 gttttaaaaa atcgttttat aataaaaaag gaggatataa aaatgctaaa tatatacata
   108541 actaatttag ggaaatataa cgaaggtgaa ctaatagggg aatgggctga acttccagta
   108601 aacgaggaag aattacaaga aatattagat agaataggga taaatgaaga atatgaggag
   108661 tatttcataa cagattttga aacagatata gaagggttag aaataagtga acatagcaat
   108721 atagagcaac tgaatgaatt agcaatgaaa cttgagaggt tagaagaata tgagattgaa
   108781 gagttaaaag ctttacttga acgtggatac attgattttt atgacatgct tgaaggggat
   108841 atttgcagat tatcaggtaa ttatactttt atacaacttg atgacaggtc accattggat
   108901 gatgaaaatt taggacgtag ttacatagaa gaaatatgtt gtggtgattt atcttgtata
   108961 gaaaatattg cttactactt tgacatagaa gcattttcaa gagatttacg ttttgataga
   109021 gatatgatta ttgaaaattt atacgaagaa gatatagaat attatgaaaa tatgacagat
   109081 attgaatttg cagagaatta tattgaacga ttaggagata ttgaagagtt aggtcaagaa
   109141 acacttgaaa gatactttaa ttatgagcta tatggaactg acttacgtta tgagaatatt
   109201 tgtatagcat caaattattt agctatagtt tcttaaataa aaagttttag ggcgttcttt
   109261 aaaaacgcca cttccaataa aaaaattgaa aggggaataa atcatgaaaa aattaataat
   109321 taagataaaa tcattgttta aaaaggattt agatagtagc tattggagta ctacagaaag
   109381 tattatttta aaatgtggaa acagcaatca attaaaatta ttgaaacaat ctaataaatt
   109441 ataaaggggg attattaaaa atgaaaataa atagcaaaat atcaaatgta gtatgggaca
   109501 taaaaacaaa agagttaaat aagcaggata tactttttat agaaaatttc aacccaacaa
   109561 tatatcataa tgatgttgat tgttacctgt ggtcacttga tgaaaaacta ccaagtggtg
   109621 tatggttgga aaatataaaa tttaataatg aaataacaga agctaaagct attgttttat
   109681 aaaattcaat tcttaagata gatgtaaaat attaggaggg ttaaaaatga taaattattc
   109741 taaatatatg actgcaacag atttaataaa tatatgtgac attttagaag gtgaaacaat
   109801 aggagaagca ggaacttata attccttaag tactagacta ggaaacttaa tcggattaga
   109861 ttgggcttta aaaggagaag aattgagaca gcaattaata caaaatatta ataatatgat
   109921 gtatttagat aatgaaaata aaattcaata taaagaattt taaataaaaa ataattaatt
   109981 tataggagga aaataaaatg aataaaataa tcgtatttta tgatatagaa gggtatccag
   110041 ttttccaaat acttgtaaga aatgtaaaaa aatatactag acaaaataga atatttaaat
   110101 tggttgatag ttttattaaa aaaaatataa ataatattaa tttaaatgat gggaaaatat
   110161 atgatagctc tagttggata atttttacta tatcaaaaac aggacattat aaattaaaac
   110221 ttaatcttga tatagactat gatatattaa ttaaaaagtt agatatcaaa aaaagtaaaa
   110281 gagaaagtaa ttctaaaaca gagagagaat tattttatat aggaacagga tggtgtaaaa
   110341 gaaaagaaat atacaattta ctaactagac aaaatcaaat tggatacgaa gattatgata
   110401 ctttaacaat agatggcgat gtttatataa gaacatataa tgaaaaatgt agaacaatat
   110461 tacttcatag taattatcaa aaaatattaa aagagatata aataaaattc aacttttaat
   110521 ataaatggag ggtttaaaat gaataagtat ttaattggtt gcataggtaa ttgcttaaga
   110581 agtatgaaag caataaaaac tagatttttt ttaatagata ataataacat tgaggatgtc
   110641 acactaaagt tgtgtaaagg agtagagcat atagcaccat ttagatataa atatggatta
   110701 gcaaattata gttttggttg tggtttatct cattatttag atacatataa tttaaacact
   110761 gtaaatggat tagatttgaa taatacacat ttaataatgc ttactttaaa tgatttaaaa
   110821 aatatagtag ggtgtaaaac tgtctttgat atagatgtag aagaaaaact attaaactat
   110881 ataaagagtt attacagatg aaaatacaag tacttaaaat attaatttta atataattgg
   110941 aggggattga aatgttaata aataaggcta aaggtattaa aacatcactt gaaacaagca
   111001 gcttaagaga actaaaagga tacataaaag atttaagaaa agcactatca ggatataaat
   111061 ataagaataa aatagatgaa attacagaat atcaaataga taaattgtgt tacaaaattg
   111121 ataatacaga tatatttgac ttgaaatata aagaaataga aaagtatata gataacttga
   111181 ttttgataat taattttatc tttatggaaa tgtaattaaa agaatgattt taatataatt
   111241 ggaggtttaa acatgaatga gtatattttg ataaattata caaaaggtaa aggagcttgt
   111301 gaccactgtg gaagaactat aaaaaatata gctacaataa aaaataatgt tacaggagaa
   111361 gtttttcatg ttggattaac atgtgtagaa aaaataatga aattgaatgt tacattttac
   111421 aaagcattgt cgagagagat aaaaaaacat tacaaatgta tggagttcta cagtaagggt
   111481 ttagatatag agacaaatct taataaaata gtgaagaata atactaaata caaagaaggt
   111541 tcttatgctt ataaatctaa tgaacaaatg ttagaagatg caattgcgga agtggcttgg
   111601 tctttggctc gtatgataga tagttgtatg agaatgaata aacttagtaa gtctggattg
   111661 atagatatag atatattaaa cagtttgttt atcaagtata aggaatatat gagtaaattt
   111721 gatgaaaatt acaataaaaa caaatataaa tataaaagtt gttatactat gaaaccttta
   111781 aaagtaatat tagatgaaaa tgaagattta aaagaactat ataaaagtat gcaataaatg
   111841 tatgtaggcg atgaattaat gacaatgaat tttatataga tgaggattat attttatata
   111901 aaagagtgtc atatctttaa tataggacat gaagtaataa aagagtttaa aattatattt
   111961 ttaataggat atatgagtta tcaaatcgct attattatag ctatattatt ataattcaaa
   112021 tgataatgat agtgatttga taaaaggatt ttaaagatta taaaaatcaa aaggtataaa
   112081 tacattaaga taattttcaa acagcttata gaggcttgta cggggtcata atcataaact
   112141 aggagggtgt aaaaatgaaa ttcaaaaata tagctatggg aatgttatta ggtttaatta
   112201 ttgttggagt tgcaggagca acaaaagtta aggctgccga attagataat ttttatgtag
   112261 aaactaccga cagagttgta gaaatattag aagatgaaag tgtagtttta tatgatacta
   112321 aagagcaagt ttataatttc tatccagttt gtctaggtga ctggaactat agctttaaaa
   112381 ataaaaaaga tttagataga gcagttgcaa cttacaaaga gctgtcaaac aatataagtc
   112441 actcaaagga tgtatatgtg attaataagt taaataacaa tggaaacatt aaagtatttt
   112501 taagtgatgg aagttcaatt gtatatataa aaaatgataa taaatattat ttttatcctt
   112561 cttgtatggg tgattggtat ttaacattag atagtaaagt tgatttaatt aactgtataa
   112621 aattttatat aggagaatga tattatgata tgttatataa aagatataca taaaataagt
   112681 ttgtatgatg catttagatg gcatacagct ggattaacat ttattattaa agatggtaaa
   112741 ttaaaaggtt ttacaataga ataaaatttt ataaaaatat ttatttgata caaaaataat
   112801 tcactattga ttgctataat aaaagtatat taaataaata tacgaatcta aactttagaa
   112861 tatactatta ttctaaagtt tagaaatata aggaggtata aaaaatggaa actttattag
   112921 taaaaaattt attaaaagaa tataaattat ggcaaggtgg agaacataga cgaatttaca
   112981 taaataaagt tccttctaaa attgatttca aagagtttat taaaaataat gattttggaa
   113041 ttggactagg agagggacta ggaataacta agatagcaca gcgtgttgaa aattctggta
   113101 atatttctga attaaaaatt tattatgatt gtaacaataa aaatttttac ttcataaaac
   113161 aatactcaaa tgtgttaaaa aaattcatag atttgcttac tgatgaaata attgaaatat
   113221 attcaaaaga aatttacaga gaagataatg tcagtcaaga aataaaagaa aattcattta
   113281 atgtattaac gttaaatggt tctgaaaaac aagttgaatt ggctaatgaa ataagagata
   113341 atgttttaaa ttttgttgac gaactagaaa agattaaaat gaaaaaaata gaagacggag
   113401 attataaagt aaaagacaag agcatagaag aaatgacaga aaaatgtaaa agaaagttta
   113461 aaagaataaa agaagaaatg agaagtatag aaagtgctaa attctttata gataatttca
   113521 aagatatttt agaacataat tctttagagc aaaaagcatt tcatataaat cagacattaa
   113581 aagattctca atttgcagaa gaaagtaaaa tatggagctt actggaatat gaaacacaag
   113641 taccacacaa attaaaaaag gttaaagatt ataacataag ttatgaagaa gctaagaaga
   113701 tagctagaga aataaaattt aacaacgcat gggctaatga gataaaagaa aatgtactta
   113761 aaatagttga aatatttgat gaaataataa aacaagttcc taatgcaagt acggaaaaaa
   113821 caaaagaact tattatagag acattaatac aagaaaatcc aagatatttc ataaatggat
   113881 ttaaagaaat taaagaatct aaagttgaaa gtattagaaa aatagaagaa atatcaagaa
   113941 aaatagaatt aatatggcat ttattttata gaggagtaaa ttgttaaaaa ttttggattg
   114001 gtacaaatat agttcaccaa cagatattat aattaaaata aaaaataatt ggaggtttaa
   114061 aacatgaata tagattttag tacaatatta gatttaaaag ttaataaaga gaatgttaaa
   114121 atgatagaaa aagagattaa taaaattaac aatgatatat ttgatataag tcttaaagat
   114181 tgtgctaact gtcacgtagg atgcgttaaa gaaacagctg gaaagagttt aaaaattgaa
   114241 acatggggtc atggtgaaat taacagtaaa ataggaataa gattgagatt taatttttat
   114301 tattcaaaaa gcgacaaagt agacatagaa aatatagaaa acattttaat atatgtaaat
   114361 agcttgtgta gcaaaaatgt agaaagtgac cttatagcta atgtaagacc taaattttat
   114421 aaattagaag ggaaatattc tacacgtgtt gaaaaatgtg aaattagaca cgaattaaca
   114481 ggcataaaca agcaggaaat taaagaattt ttagagtatc ttacagaaat tgaaaagagc
   114541 gaaagaactt ttatatataa aaataattaa aaaatagaga tggctaaaat ggaaaataaa
   114601 ataatagaat ttttagaaga aatggaaaat ttaaaagggc aagagtttaa tataatagaa
   114661 tttgacaatg aaatacaaga attatatgct agagcagtag aaagaacaag tagttgggac
   114721 ttttttacat ctggagatat aaacgaatat ttaaatgtag gaagctatac atattgcgga
   114781 ttaggcgaag attatgactt agacataaat attgaatttg aagtggtaga aaaaaacgag
   114841 gaagatgttt gtagttctat aataaaaata ataaatatag agaaattata aagaggtgaa
   114901 ttgatatgca aaaatatttt aaagaagagt ggcattatat acctgatttt agatttaaaa
   114961 tagaaaatat agaaaattgc acaattaaag ttaatgattt aaaggtagat atgaaaatga
   115021 gtcaagattt tagatttaaa ggatatatag aatatactat atatatacca tataaccata
   115081 aaatttttac aagagagtat gatgaaattt tagacttaga gcagcctagt gaatgtgaac
   115141 aattttgtga cgaattatca gaacttagag gtaaagttga agaaggtatt gagcagtatc
   115201 taaaggataa aaataaatta gtagaaatag aatactaggt gtaattaaat ttattttcaa
   115261 aaactataaa atagaattta aaaataggag gaattaagat gaattataaa aaaataaaaa
   115321 gatttttaaa aataatagat aagaatatag ataaaataga agaagaagct ataaaagttt
   115381 ataaggaaag ttttttagca gaagataata ctataagaat atatataaat ttagaaggta
   115441 aagttgaagc tatatcagtt cataactcgc ttaaatatgt agaagatatt tatgaaagag
   115501 aaatttttat ttgtgaatta aacaaagaaa aaataagtgt taatgaatta ctaggagaac
   115561 cttgttactt aaatgattat gaagaatttg aaaaatggtg taaaattaaa aatgaagatt
   115621 tggattggga aagttataaa aaattcaaca aagacagctt tgacgaatta attgaaagaa
   115681 atctagaaga tagtttgtca gatttttcag aagaaataaa aaagaatatt gaaaatcgta
   115741 aacaagaatt gcaagtaata gttgaaagaa gtatagaaaa ttattattat catgatatta
   115801 ttctttttaa taatttgctt aataataatt cgcttaataa taattgattt aacttgtgta
   115861 ttaaatcgca ataaaataat aagagaagca tcaaaagaaa attaaatgat gaaaaatgtt
   115921 ataagacaag ctatacttat aatgtacaat atgaaaaatt ggaggaataa atatatatgt
   115981 ttaaaataga agtaggaaag aaattagaag cagaaatacc agttggcttg agtattaatt
   116041 ttagtgaaac tggatttaca gctatattta aattagataa tttatcagac aaagaaataa
   116101 ttgaatttcg aaaaggtaat ttaagaatag atgtatcttt tttagacaaa attattttct
   116161 tcgtatttac aaatacaatg ggtataggag atgcagatat accatttaca attcatttat
   116221 ctaaatgtaa agaatttcaa gaacttgaag aaaatgaagg atatgcaatg gatttaatgt
   116281 tagtagaagc taacaacaat attgtaaaag gtttcagacg tgttggactt aatacaaaca
   116341 catctaaata tttaaaaaaa tgtgctatgg aacaactaga atatgatttt aatatggatg
   116401 aacatatgag aagggttaaa gaaatacaaa gaaaatacaa ctcaagagaa ataaaaaaat
   116461 tatcaggagc atattctaag tttgaaagaa agcagtaatc aaagtataaa ttatatattt
   116521 taattagtat aaacaacaat aaaatatgct tttatcaggt ctgcatttaa ataatgcaga
   116581 cttttaaaca gaaggagttt atgaagatag ttaatatata aaaagtaata aacatagata
   116641 gttattatta ctttaaagta ttttgggggt taatatgaaa aaagaattat tggctggttc
   116701 atgtaattta aataaaaagg aacaaacact agtcggacat attaaagatt tatttaaagt
   116761 attagaatct atattagata tagatttgta ttcaggtaaa gatatagaaa tactaaagat
   116821 atgttgtgtt ttgcacgatt taggaaagat taatttaatc acacagcaaa aaatagaaat
   116881 taacaataaa atatcatcta gttctttaaa agaagaaatc aaaaaattag aatttgaaaa
   116941 aaaatcttta gaaaagatag cgaaacataa tgtgttttct ggagcatttt tgaaatcaat
   117001 attagaaaaa atgaacttgt cagaagaaga tgaattttat atatataaaa gcataatgct
   117061 acatcatgga aattatatag attatatgaa attaactact agtaaagtac aagaagaaat
   117121 ttataagtat attgatgatg gaattttaga aaacaaagaa tcatttaatc tgaaagatat
   117181 agaagattgt ataagtgaaa ttttaaatat agattttaaa tttaatgagg atgtactgga
   117241 ttatgattat ttagataaat taagtgaaaa aatggttata gagagtgatt atgataatgg
   117301 tcaaatagat gactcaattt taaatcaaag aagagctaaa tatattttgt ataaaggtat
   117361 gctgaattta atagaccatg ttgcacaagg cacaaataaa gatataaaat tttataatga
   117421 ttttactgat aaagaaattg atgaaatgat tttaaatgat atatataata atcatgataa
   117481 tttagaaaaa aataatatag agttcaatac tatacaaaaa aagatgagaa aattttctga
   117541 aaaaaatgtt ctgactgttg catttactgg gtcaggaaaa aaagttgctg attgtagaaa
   117601 gaattttaaa agaaaattat ttttagttcc aaatcaattt tcatccgaaa gtttttacat
   117661 aaaaaatata tttaaaaatg attatcttgg attacttaat ggagatataa atttatattc
   117721 atatagtgaa aattataatg aacaagattg tgaagtaact ttaagagata tagatttgag
   117781 tttgaatctt tgtaaaccat atttaattgc aaccattgac caattattat taagtatgtt
   117841 taagtttcca gggtatgaaa agatatttgc agctgtaaaa aatgcatcta taactgttga
   117901 tgaagttcat ttattgaatc caaagatgtt tttaatttta atatatttta tacagtttgc
   117961 atgtaagtat ttaaatgttg attttcactt aatggcaact atgcttccaa aatcatataa
   118021 agagcaaatg ataagcaagg gaattatttt tcaagaagaa tgtaatgaaa atatcactga
   118081 taaaggggag atagttttta ttgagagcaa caaggaagaa gatatttgtg aaggtaaaaa
   118141 tgtaaaggtt tcttttataa aagaaaaaga aatcaaatca atagttgaaa atgctttaga
   118201 aaagaaacag agaattttaa taataaaaaa cactattgat tcagctaata gcacatataa
   118261 gcttttaaaa gaaaacttat caaataaata taatgatgca gatataaatg ttttgcatga
   118321 taggtttaaa tttaaagata aagaagaaaa atataataaa atatttgatg aaaagggtga
   118381 tgtttggata tctactcaag aaatatcatt agacatagat tttaatatag taataagtga
   118441 tttagcaact atggatagtt taatacaaag aatgagtaaa tgtaatagta atgataaatg
   118501 tgaatatggg aatttttata ttctgcctag taaagatgaa atttatgacg acaaacttaa
   118561 gaatacaact aaatctattt taaataaaat attaaagaca gaatctattt ttacgatgag
   118621 tattagaaaa aatattttga atgaatatta tgataatagt gtagttaaaa aatattttga
   118681 agatagtttt ataaattgtg ataatgaaat taagaatatt tttggtatgt ataaagctga
   118741 tttcgatggt ttggatttaa tatttaattt tgaaccttat aaacatattg ttgataatga
   118801 gagcgaagca tctaagcttt ttaaagctac tgatacaaac tgtaaaatta ttctcgagga
   118861 agatttttat cgagaagata tagatttaca acgtgactca attcaagtgt caggatttat
   118921 atttaataga ttgtgttact tgaaattaat aaataaatta gaaggatata tggtactaaa
   118981 atctagtaat aagttaaagt ataacagcac tttagggtta attttgtagt aagtatataa
   119041 agtcaatata ctaaattaca gcatattgac tttatcattt acttattaat agatgtaata
   119101 ttgtaaaaaa atgatatagt ttagaaagat atgtagatta aacgtagaat taaaagatta
   119161 acatagtatg acaatcgtaa tctcgtgtag tatacttaaa ttgaggtgag tatatgactt
   119221 ggaatgattt aaaaaataat gatataacat atatagttga atatattaat agtaagttaa
   119281 atgatagtaa gagtttgact aaagttgcta tagagcttgg tgtatctgaa agtagtataa
   119341 gaaaatatct cactaaaaga ggttataaac gtataaatga tgagtatgtg tttattggtg
   119401 acaatagtat gacaaaagta gtaaacaata gacaacagag taataatgat aatacattta
   119461 atatgacaat agaagatgac aacaatcaca acatagtaat tgacaaccaa tttaaaaata
   119521 atataatctc tttagcaaaa gactatgata aaatacaaaa tatattaaat tggtttgaga
   119581 ataaagaaga tacgaatgta atagaagttg tacaggatgg tattaagatt gatttaactt
   119641 caaaagatgc tataaggact acagttaggt tgaataaaga tgcatggaat ttatttgatg
   119701 agttttgtga aaaattcaga gagtttaata agtctgattt aatgagtatg gcattacttg
   119761 aatacataaa gaagtatgat aaataaaaat gacaataaaa tcgtagtttt aatttaaata
   119821 acctcattat tttgaggtta ttttttttat tttctattta aaacggagaa ggtaaatgtt
   119881 ataatatata tataattaaa aaaaggatgt ttgaatttat gaattataat ttaaaaaatg
   119941 aaaatgtatt atcagagcta ttacaaaatg aggatgaaaa aacaaggatt ggtatactca
   120001 aagtttataa agtgttaata ttttggtttg ctaaagaagg aaagcttgtt gaggaagaaa
   120061 atgactatat tgttgagata gtaggtaatg ataaagaaga aattgtgaga gatatgagcc
   120121 agaataaaac atacaatgcc ataatggcta taatttttaa aattaataag atatttaaaa
   120181 ctttaaatta cgatattcac ttttctaaac caaaaaattt agtcgctgaa tttgatttat
   120241 caaaatatct aacacaagat gaagtacttc aggtatgtag taagctaact aatgcacaag
   120301 ataagtttat aatttatgca gctttttgtg gtcttattgg taaagatgct gaagacctaa
   120361 ggggtataaa aataaaagac atagatttag aaaaaagatt aataaagtta aaggatacag
   120421 aatttaagct ggacaagttc ttatttgatt ttacaaaaga tacattagaa caaaaagagt
   120481 ataaacaata tttggagcaa gacagcaatg ctgctgaatt ttatctattt aattctaatt
   120541 ctgaatattt aattaaaact aaaccagatg ctagatatgg aaatggatta aaagagatgg
   120601 gatactcaac aattcgcaaa agattactta ttattaatga ttatttagat aataaagtac
   120661 agttgaattt cactaattta aaaatttctg gtattatgca tcaaatgcat ctaaatgaaa
   120721 aaaaatatac tcaatttgga tattggacta ataaaagctt gaaagagttt aaggaggata
   120781 acaatttaaa cttcaaaata caagaagttc tatctattta taagcaaaaa tatattaagg
   120841 agtaacctta tgagtaaaga tgttaaagaa aacataagta atgaacaaaa agatttaaat
   120901 aaaataaatt taatctatca tattatatat gacaatgata gatttaaaat gataacaaga
   120961 aaatattttg aaatttttag attaaagggt atgtctgctg aagaaataag tttaaaattt
   121021 gaagaatttc tatattctct tatttcagat aagtttaaaa agataaatac agtaaatgat
   121081 gttaaaaaat taagaaatca agagaactca tcatcattaa aacttaagaa gagatggtct
   121141 gatatgactt tagattctct aagtgaagat actaaatctt caagaaaaaa aagcagtaaa
   121201 tacctaaaaa aagaattaga aattgttgaa agtgaatatt taaaaaatgg aagaatcatt
   121261 agtgctggag taaaaaatcc aaaagaatta aaggagcaaa gaaaagattt tttagatgag
   121321 tggaagtcaa gctctagtta taacatatgt gattacccat atttacaaga gttaagtact
   121381 agcaaaataa attccgcatt tatacatgat atactaatgt gtataacaga agtactaaaa
   121441 gatgattatg attttgatat taataaaatt gcaatcaaaa ctccaccaca tatttcacct
   121501 ggaatttttt tacctttcac aaaaggtaaa agatactcgc acttgaatgt taaaaaaaat
   121561 gaaaatgaat acatgtcaga cgactatagt gctaagaatc tttcaggtaa acaaatgaat
   121621 tttttctata agttttcaat tgggcaagat gaaaatattg aaaatcttaa actagagcta
   121681 ataaatcctg atacagaaaa aagggctttg ccaaaaatat atttagataa acaagacctt
   121741 gaaattatta gatttgttta tacttatagc cacatgaact ctttttcatt ttatttaggc
   121801 gatttagtaa agtacttagg actttcagat ggaaaaaaga actatattaa tatcaaaaat
   121861 agactcttaa aattacctta ttatactttt tattcgaatc aaactaatga taaaggcgaa
   121921 ttaaagtatg agattagttt taatttgttt tctagtacaa caatagtaaa tgatgaaaac
   121981 aataataata gagagttggt taaaatcaca aaatctttta ttgaagaagt cgaaagagtt
   122041 aatacagata taatgtatag aaatgaactt gaaaaattac aattaacaca gtcaattaat
   122101 ctagcttatt tcttagaggg tagaaggtct tttcttatat cttgtggtga ggatataaaa
   122161 aataaaagtt ttagatatga catagaagat ttaaaatttg atgttcatct taataaaagt
   122221 aaaacagtaa agcaaaatat ggagtattta gaactaggtt ttaatgagat aaaagaaaat
   122281 caatttataa ttaaagacta taaaagagga aattcttatt ttgatgttta tttctatgaa
   122341 gattttgaaa aaagaaaacg tcttataaat aatactatac tttctttacc tgactacatg
   122401 atagaaaata aatcttaaaa atggccatat attctccgta cttaacaaaa aatgtcgaat
   122461 ttattttaaa taaacttaat aaaaaaagta aaatgaccgt atattctccg tacttaacaa
   122521 aaaatagatt tttcaaaaaa actaaatgac cgtatatctt ccgtacttaa caaaaaatag
   122581 accttttttt aattatttcc ttgtcgaaaa attttttttg taacatattt tttacttttc
   122641 ttagtaattt caaatctcac gtcttctgaa atcactatgt ctgagctaaa tgaccgtatc
   122701 agctccgtac ttaacagaag acgttgaaac cactctattt gagctaaatg accgtatcag
   122761 ctccgtactt aacaaatata gacataaatt tattttttgg gtaagataaa tttgagtaaa
   122821 aggaatcatc gccaaaatgg ctatatcagc tccatacttc tgaaattatt gatttttcaa
   122881 aggagaaaat tatgagtctt aaatataaag ataaagaatt tgtacttgta gaaaaaaaga
   122941 atataaatga attagataac tctaatataa aatatattga cctaaaggac aagcagtatt
   123001 atgtggttac acaaggcaaa aaacctaaaa gatttaacct agaagatatt aaaaatataa
   123061 aaaaagattt agataatggc ataagtttaa gaaagtgtgc aacaaaatgg aactgtagca
   123121 ctaggactat acaggatata aaaaataata tttattaata tacagaggta tttatttact
   123181 ttatacctca ttaagattat aataatcata tatagagaaa ggaggattat aaaatgcaag
   123241 aattaaaacc agttgctaga atatcaaagt caaaactaaa agatatatta gatgttttta
   123301 aaacttctat aagaaaagta gaaaaaatag attattctgc ttgtaattct tgtgcaataa
   123361 aaaataatcc ttcttttgat agtcataaat taaatgaatt ttgttcaaat tgtcctaaat
   123421 taaaaactat atcagagttt tcttatgtta cagaaaagga aacagaacta ggtacaatag
   123481 aaattgtaac taatgaaagt aaatgtatgg ctttaagtaa aagtgctata aaacagtatt
   123541 tagcatatca ttttatagta tctaataaaa attatgttag aaaagcagta tccttccaaa
   123601 atatagctga tatatgcaat gtttccattg ttacagccag acataaccat aatgtattac
   123661 tacgagttgg attagtttat tctacagaaa taaaaagagg taaatttgat atagtcatag
   123721 atgaagagta caaaaaccat cttaaaaaag aagatggtgg aaaaggttac ttaactatgt
   123781 cattagatac tttaaatcac ttattaagtt ttgaaaatgt aaatgagtta aaacttgaaa
   123841 ttaaaaaatt aatttgggct gatgctaaag gaagtaagtt aggtaaaaga atacgattta
   123901 aaaaagacaa tcttatttct atacttccag aatacataaa gaaatcaaac aagcgtacag
   123961 aagccttttt aagaagttct aaaactttat ttgatgtaag taatgacaca atagatacaa
   124021 agcgttatga gacgaaagaa actatctcag agaaatatac agaaccaatt agaatacaaa
   124081 tatcgaaatt ctttgataat actggttgct ctttgagttc taattatagt aaagtgttat
   124141 ctcagataaa tttagataga tataatttat ttgaagaaga tttaagaagt aaattagaca
   124201 tggaaaaaga tattattata aatgacttag ttggattgtc ggtgcagtat ggagttacta
   124261 gagttttagc agcattagat tatatgttta ataaatatac agttgatgaa gaagatataa
   124321 attataatga aatacgtaat cctggtgcat ttataagaac attgattgtt cagagcataa
   124381 ataaaaatgg aagtttagca atagcaattt aatatatgga gtagttcaaa aaaaatgatt
   124441 ttggctactc ctattagtgt ttgtattcaa attataaaat acttaaatag agctataaag
   124501 agcgtgaaat ctctattagc tctatttttt tatgcctatt ttaatgaaat tatatacaca
   124561 atagaaagtt ttttatacat attactaatt taattttaca ataataatat atatttattc
   124621 aatttattaa gaaacttatt
//
LOCUS       DBGJFF010000002       106824 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.002, whole genome shotgun sequence.
ACCESSION   DBGJFF010000002 DBGJFF010000000
VERSION     DBGJFF010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 106824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 106824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..106824
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.002"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(67..918)
                     /locus_tag="ACOIAF_000165"
     CDS             complement(67..918)
                     /locus_tag="ACOIAF_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HGZ2667201.1"
                     /translation="MALVTTTSMLKKAQDEGYAVGAFNVENMEMVIAVIEAAEELNSP
                     VILQTTPSTVKYAGLDYYLANAKVAAERVNIPVAMHLDHGSSFDLAMKALRSGYTSIM
                     IDGSHESFENNISISKSVVKACSPSLIPVEAELGKVGGKEDDLDGGNSNDYTNPKEAK
                     EFVDRTGISSLAIAIGTAHGLYKGEPKIDLDRLSEIKNVVSIPLVLHGGSGIHDDIIK
                     EAIKRGIAKVNYATELRIAYSKGVKKVLDEDSEVIDPKKYGLAGLQLVKDFVKEKIKV
                     CGSVNRV"
     gene            complement(967..2130)
                     /locus_tag="ACOIAF_000166"
     CDS             complement(967..2130)
                     /locus_tag="ACOIAF_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HGZ2667202.1"
                     /translation="MFKLEEQKLKDLGAIITTNEIKQQPELWLETYEIYKSNKEKLSR
                     FIDAISNNHGQFRVIFTGAGTSAYIGNSILPYLKNKNDIRKYIFEAIPTTDIVSNPYD
                     YLKKDIPTLLISFARSGNSPESLAALNLGNKIVDNFYHLAITCNPEGELAKMTKNDEN
                     NYLLLMPSKSNDEGFAMTGSFSCMMLSAMLIFDSLEDDVEKSYINAIIEMGRNVIDRK
                     DEIHELINKDFDRVVYLGSGGLGGLTQEAQLKLLELTAGKISTVYDSPMGFRHGPKSF
                     IDENTLVFEFVSNCLYTRKYDLDVLEEIKRDKIAKFTCVVSVENENNFSGTKFEFKEK
                     YNKLPDVYLAMPYILFAQTIALFVSVKVGNKPDTPSATGTVNRVVKGVTIYEY"
     gene            2607..3524
                     /locus_tag="ACOIAF_000167"
     CDS             2607..3524
                     /locus_tag="ACOIAF_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HGZ2667203.1"
                     /translation="MKNIKMGMIGLGSIAQKAYLPILTKSERFEFVGAFTPNKVKREK
                     VCSDYRIMPFDSIESLAKKCDCIFLHSSTETHYEIIKILLNLGVHVYVDKPLASTVSQ
                     GEELIELSTKKNLNLMVGFNRRFCPMYKEIKNNATEIVSINICKHGLNSLRNVKFDST
                     LIDDYIHVIDTALWLANEDVEISGEDLFLTDNKNLIFVSHKLKGKNFSINTSMHRDSG
                     TKLEQVEILSKGNIQRVKNLNVLEIEEGGNLTLKQSGAWVNILKQKGFEDISNHFIDC
                     IENNIKPAINGEECIKAQRLLEKIINSVK"
     gene            complement(3653..4582)
                     /gene="pfkB"
                     /locus_tag="ACOIAF_000168"
     CDS             complement(3653..4582)
                     /gene="pfkB"
                     /locus_tag="ACOIAF_000168"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HGZ2667204.1"
                     /translation="MITVITFNPSIDRMYRVNNINIGEVQRVVSKNATAGGKGLNVAK
                     VCKILKENPLAMGFLGGFNGEFIKNELRKLDIQNKFTKIEQETRNCLNIIEDNKVSTE
                     FLEKGPIVEKSDLERFENDIEEVLKNTKILVASGSYCENMPIDYYEKIGNICRANNIK
                     FILDTSGEALKIALKSKPYLIKPNIDEIRHLLGMNIESREEIILSGKKLIEMGAENVC
                     ISLGKDGMVYLNEDNVYDVKVPVVKCINTVGSGDSTVAGFSVGILRGYKIENLLKFAN
                     ACGISNALNMETGFVDLVEIDKYQDLVEVKKLS"
     gene            4849..5574
                     /locus_tag="ACOIAF_000169"
     CDS             4849..5574
                     /locus_tag="ACOIAF_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGZ2667205.1"
                     /translation="MIKRNDKRPIYDQLVEILRTKIENEMEPNDRMLSERKICDEYGV
                     SRTTVRLAMAELEHMGYIYKRHGKGTFVAALSQNSQNLMESYSFTDYMRTQGKKPGTK
                     VLSFEVVESTKYFSEKLGITPGEKMIKIMRLRLADDLPMMLERTYLPMKEFIGLTKEK
                     IEQKPLYEIFREDYGEIIKVADEEFSAGIISDKEAKLLEVPGDSPCLKLLRTTYNDDN
                     IVIEFTLSVARSDKFVYRVRHVR"
     gene            5657..6811
                     /gene="nagA"
                     /locus_tag="ACOIAF_000170"
     CDS             5657..6811
                     /gene="nagA"
                     /locus_tag="ACOIAF_000170"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HGZ2667206.1"
                     /translation="MKSFIYADKFFMKHGIKEKGYLSIINGKFGEFQQERPNVTDNVI
                     DFSGKYIAPGLVDTHIHGLLGADVMDNTFEAINTISKGLLKYGVTSFLPTTLTDSVDT
                     LSDSVENISNSFNRVEGAKIQGIYLEGPFFTEKYKGAQNEKYFKDPDINILKLWQKKS
                     KNLIRKIAIAPERKGSIELTRYATKNNISVSLGHSSATFEQVKQVVNSGAKVFVHTYN
                     GMSPLNHREPGMVGAAMVLKDTYAELICDGHHVSPIAVKIMMDAKSSNNIALITDCMR
                     AGAMEDGKYTLGKFNVNVKNSVARLNSGSLAGSVLTMDKAVRNIVDWNISTLEDAIKM
                     ATYVPALSCNIDNVCGSIYQGMDADFIVTDNDFNIHETYINGICKYKVNK"
     gene            complement(7520..7990)
                     /locus_tag="ACOIAF_000171"
     CDS             complement(7520..7990)
                     /locus_tag="ACOIAF_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HGZ2667207.1"
                     /translation="MYNNIKNILKENHITFEEYDHEPILSFEKAKEIREKLQYTGVES
                     KSLFIKDRSGNYYIFVTTEGKKLDPKTIKNVLGGKVSICSKEELTQKTQCLPGCVSPF
                     GYEKNISLVIDQDIFNHDKIIFSPGIPEKTFIITASGFKKVINNINNTTYKYIY"
     gene            complement(8594..9751)
                     /locus_tag="ACOIAF_000172"
     CDS             complement(8594..9751)
                     /locus_tag="ACOIAF_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HGZ2667208.1"
                     /translation="MYLVISKKKAIFLGVILVIITAMVTSAFQLTLGNKVVISKELYE
                     DYKKYDKLLGLESIIKQDFYKKVSDTDLVNGASKGLFLGTNDKYSGYYTKDEMENLIN
                     DSEGSYVGVGMYIGASKDGGLVVVPMKDSPAEKAGVKSGDKLVKVNGKSVSYKNSDEA
                     VRMMKGKKGKIVELTILREDKQLNFKVKTDQIIEKSIESKVIDNDLGYIEITQFISST
                     YTDFDEALKELKAKNIKGLVIDLRNNPGGMLDICKEVADELIGEGTIVYTKDNKGNTE
                     YLKSDKEKLGLPIVVLTNGESASAAEILTAAIVDNKEGISVGTTTFGKGLVQSVVRLK
                     DGTGYKLTTAQYFTPNGDYINEKGIKPTIEEKDENKQLDVATKWLREQIDK"
     gene            9905..10474
                     /locus_tag="ACOIAF_000173"
     CDS             9905..10474
                     /locus_tag="ACOIAF_000173"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HGZ2667209.1"
                     /translation="MKKEFRKKVIESRKNKSANFVISNSNLITEKLLQMEDIRNATNI
                     MLYLNFNNEVQTDNLIKKLLSLKKIVSSPITIKENHTLIPTQITDLKEGLRIGEYGIR
                     EPNEKSPTVDIKALDVVIVPAVAYDIHCYRLGYGGGFYDRFLENLRKDAITIGIAFDF
                     QIFDSIPKETHDAQLDYIVTETRIITPNS"
     gene            complement(10656..11072)
                     /locus_tag="ACOIAF_000174"
     CDS             complement(10656..11072)
                     /locus_tag="ACOIAF_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667210.1"
                     /translation="MNVKFNIKGIIYGVIALVFVIGAFIFIPSMFVEKSKPIDYTVLQ
                     RNSIPEKILDVMDKYTNEERALAAKIDNKIYIIVTRGNNKYGIEMDKIESVVEEGKDV
                     LRVKIKYKDKEDSFPYIVVETNMSELPDRIELNATK"
     gene            complement(11297..12391)
                     /locus_tag="ACOIAF_000175"
     CDS             complement(11297..12391)
                     /locus_tag="ACOIAF_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667211.1"
                     /translation="MSEKVNVKTVISFAGAYVATVIGSGFATGQEIMQFFSFYGFAGI
                     IGGIISMVLFSWMGASVMSKGKELQLKEPIKIYRVYCGKYLGIFFEWFGPLFLFGVFV
                     VMISGAGATLTEYYGLNPFVGRVGMAVVALLTVSLGLDRLSKILGGIGPIIIIFTLLV
                     GGISLAKNIGNIGEAAQVLSTVKIAKPVPNAYLSGVIYTTYNTIVVMAFLTGLGASAA
                     NKKEAICGGILGGVALMAAAIMMHLAILSDIGNLYSKAIPSLFLADKISPIIGVLFSI
                     ILILGIYTTAVPLLWSVTNRFVDDNHPKFKLITLIAAILGLIGGFLPFDKLVGILYPY
                     TGYMGVIILVCVLYRQITKTSGYQEGISDK"
     gene            complement(12814..13755)
                     /locus_tag="ACOIAF_000176"
     CDS             complement(12814..13755)
                     /locus_tag="ACOIAF_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HGZ2667212.1"
                     /translation="MSKMATREAYGKALVKLGQINDNVVVLDADLSKSTKTHDFYKTF
                     PDRFFNMGIAEQNLIGAACGLSTAGKIPFASTFAMFATGRAFEIIRNSVCYPKLNVKI
                     CATHAGLTVGEDGASHESVEDIAIMRAIPNMTVLVPADGVETEKIIFEIAKYNGPVYV
                     RLGRSSVPVLFDEDYKFEIGKGTVLREGKDVSIIACGIMVNEALLAQEKLQEEGISAR
                     VINMSSIKPIDKDLILESAKETNAIVTVEEHSIIGGLGSAVSEVVGESCPTIVKKVGI
                     KDTFGESGTPNELLKKYELTCDDIVKTVKEAIIAKRM"
     gene            complement(13748..14575)
                     /locus_tag="ACOIAF_000177"
     CDS             complement(13748..14575)
                     /locus_tag="ACOIAF_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HGZ2667213.1"
                     /translation="MRDHKGLNEIACIIRRDIVSMIHRAKSGHPGGSLSVVEILTALY
                     FDEMNVDSSNPKMEDRDRFVLSKGHAAPALYATLAEKGYFDKEEMNGLRKIGRMLQGH
                     PDMKGTPGVEISTGSLGQGFSVACGMAMASKLDNAPWNVYTLLGDGEVQEGIVWEAAM
                     SAAHYKLDNLIAFLDNNGLQIDGDIESVMSLGSIVDKFKAFGWNVIEIDGHDFDQIFA
                     ALDIAKSTVKKPTMIVAKTIKGKGISFMENQAGWHGTAPSDEELEKALLELGGADNE"
     gene            complement(14746..15129)
                     /locus_tag="ACOIAF_000178"
     CDS             complement(14746..15129)
                     /locus_tag="ACOIAF_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HGZ2667214.1"
                     /translation="MKGRLPKVSTNCEIKRGDLYYADLSPVVGSEQGGVRPVLIIQND
                     VGNKYSPTVIVSAITSQINKAKLPTHVEISSNEYGLNKDSVILLEQIRTIDKKRLREK
                     IGCLDENMMIKVDDGLQISLGLFTF"
     gene            complement(15098..15385)
                     /locus_tag="ACOIAF_000179"
     CDS             complement(15098..15385)
                     /locus_tag="ACOIAF_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HGZ2667215.1"
                     /translation="MYDKSKEKIEVTLPDSLISEVDSIVQMENSNREDFAKAAFQFYI
                     TQKKKIHIKESMKTGYREMGQINLSLAELGMTVEEVSSDDKNEGKIAQGEY"
     gene            complement(15418..16575)
                     /gene="alr"
                     /locus_tag="ACOIAF_000180"
     CDS             complement(15418..16575)
                     /gene="alr"
                     /locus_tag="ACOIAF_000180"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HGZ2667216.1"
                     /translation="MQKITVPTWAEINLDNLRFNLNNIKNLLEEDIKICGVIKADAYG
                     HGAVEVAKLLEKEKVDYLAVARTAEGIELRQNGITLPILNLGYTPDEAFEDSIKNKIT
                     MTVYSLETAQKINEIAKSLGEKACVHVKIDSGMTRIGFQPNEESVQEIIELNKLEYID
                     LEGMFTHFATADEVSKEYTYKQANNYKFMSDKLDEAGVKIAIKHVSNSAAIMDCPDLR
                     LNMVRAGIILYGHYPSDDVFKDRLELRPAMKLKSKIGHIKQVEPGVGISYGLKYTTTG
                     KETIATVPIGYADGFTRIQKNPKVLIKGEVFDVVGRICMDQIMVRIDKDIDIKVGDEV
                     ILFGEGEVTAERIAKDLGTINYEVLCMISRRVDRVYMENNELVQINSYLLK"
     gene            complement(16590..17180)
                     /gene="gerS"
                     /locus_tag="ACOIAF_000181"
     CDS             complement(16590..17180)
                     /gene="gerS"
                     /locus_tag="ACOIAF_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="germination lipoprotein GerS"
                     /protein_id="HGZ2667217.1"
                     /translation="MRKKWTIVCIMFLALVIIVIGCQKRQSTKEEVYKDFQKQISDMN
                     YYSCKAEVEVVGNKSPHNYVLIHTYKKTDNYKLEVISPKHLKGKSIEYQGDKILVKNP
                     KISDVVELPNTGKNNQYLFVGDFIKNYLQNEEMKVKLSKGHLVLETFIPGDNKYFNKQ
                     VLYVNADTKNPEKMEVLDKEGVPRFTVKYKDFEYRN"
     gene            complement(17201..17662)
                     /locus_tag="ACOIAF_000182"
     CDS             complement(17201..17662)
                     /locus_tag="ACOIAF_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HGZ2667218.1"
                     /translation="MMDKTAKEIMTTDVIVAKQDDSIADVANMLIAEKIGGLPVVDSE
                     NRVVGIISETDILKKEKYIEAPLYINLLQGLIFLDDLKKVEKDIKQVAAYKVGELMSK
                     DIIKVHEDDKFDDVANVMIKKSINRVPVVDDDNKLKGIICRYDIIKALYNE"
     gene            complement(17677..18057)
                     /gene="acpS"
                     /locus_tag="ACOIAF_000183"
     CDS             complement(17677..18057)
                     /gene="acpS"
                     /locus_tag="ACOIAF_000183"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HGZ2667219.1"
                     /translation="MNIFDIGVDIIEIDRIRKAVDKNNRFLEKIFTDREIEYFNSKNF
                     KAESIAGNFAAKEAISKSIGTGIRVFNFKDIEVLRDEMGKPIVKTYNNLAKMCIDYNV
                     LEIKVSISHSKDYAIANAITIIKD"
     gene            complement(18519..18779)
                     /gene="atpC"
                     /locus_tag="ACOIAF_000184"
     CDS             complement(18519..18779)
                     /gene="atpC"
                     /locus_tag="ACOIAF_000184"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HGZ2667220.1"
                     /translation="MASEFSLKVVTPSNTFYDGDVEMIVVRTTKGDKGVLKGHIPFVS
                     GIPVGTLKIKKEGSFKEAKIAEGFINVTQDKTVILTESAEWI"
     gene            complement(18782..20176)
                     /gene="atpD"
                     /locus_tag="ACOIAF_000185"
     CDS             complement(18782..20176)
                     /gene="atpD"
                     /locus_tag="ACOIAF_000185"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HGZ2667221.1"
                     /translation="MANVGKVVQIVGAVLDVKFDSEQSLPNLLNALVIKLGDKEIVAE
                     VAQHIGDDTVRCIAMSATDGLVRGMEVVDTGGPISVPVGDETLGRIFNVLGKPVDGKP
                     APKSAPKLPIHRPAPAYDELETTAEILETGIKVVDLLAPYLKGGKIGLFGGAGVGKTV
                     LIQELINNIAKQHGGISVFSGVGERTREGNDLYGEMSESGVINKTALVFGQMNEPPGA
                     RMRVALTGLTMAEHFRDEQGQDVLLFVDNIFRFTQAGSEVSALLGRMPSAVGYQPTLA
                     TEMGALQERITSTKKGSITSVQAVYVPADDLTDPAPATTFSHLDAKTVLSRQISSLGI
                     YPAVDPLESTSRILDPSIVGKEHYEVARGVQSILQRYKELQDIIAILGMDELSDEDKL
                     IVARARKIQRFLSQSFTVAEQFTGNPGQYVPVKETVRGFKEILEGKHDDLPESAFLFV
                     GTIEDAVRKAKGSM"
     gene            complement(20189..21067)
                     /gene="atpG"
                     /locus_tag="ACOIAF_000186"
     CDS             complement(20189..21067)
                     /gene="atpG"
                     /locus_tag="ACOIAF_000186"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HGZ2667222.1"
                     /translation="MAGAGIKAIKTRMGTVESTKQITKAMELVASSKLRKAKEKAEDS
                     REYFNTLYNTVKDIAQTTRGIRNEFLKEREVKNKCYVVVAGDRGLAGGYNSNVLKESV
                     SHMKGKKETVITVGKKAHEFFSKRNYNIAKDINSVEACGYEDATEISQTIIDLYKKGE
                     VDEVYIAYTKFKSALSQEVEILKLLPLVFEKDTDEQGMAVKEEKQNRSKVQYIPSAEA
                     VLSYIVPQFISGTVYGGILESFASEQGARRTAMESATDNANEMLSNLELSYNRARQSA
                     VTQEITEIVGGVEALK"
     gene            complement(21095..22597)
                     /gene="atpA"
                     /locus_tag="ACOIAF_000187"
     CDS             complement(21095..22597)
                     /gene="atpA"
                     /locus_tag="ACOIAF_000187"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HGZ2667223.1"
                     /translation="MNLKPEEISSIIKQQIKNYENKVELTDTGSVLTVGDGIASVYGL
                     EKAMSGELLEFPGEIYGMALNLEEEVVGAVILGDDSEIKEGDIVKRTGRIVEVPVGEA
                     LIGRVVNSLGQPIDGKGPIAYTKTRPVESEAPGIIDRRSVYEPLQTGIKSIDSMIPIG
                     RGQRELIIGDRQTGKTSIVIDTILNQKGKDVICIYVAIGQKRSTIAQLVSSLEKGGAL
                     DYTIVVSATASESAPLQYIAPYAGAAMGEEFMYNGKHVLIVYDDLSKQAVAYREMSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDELGGGSMTALPIIETQAGDVSAYIPTNV
                     ISITDGQIYLQPELFYSGVRPAVDPGISVSRVGGSAQIKAMKKVAGTLKLAYSQYREL
                     AAFSQFGSDLDEDTKKRLAQGERIVEILKQGEHQPIKVENQVMIIYAVINNHLEDIPI
                     DNIARFESELYAFVDNNYPEISRKILGGEDFTHDLTDAINEFKEKFVVEV"
     gene            complement(22614..23159)
                     /locus_tag="ACOIAF_000188"
     CDS             complement(22614..23159)
                     /locus_tag="ACOIAF_000188"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HGZ2667224.1"
                     /translation="MINVIANRYAEALFQLGEEENSTDVLFKELEKVVDMMTKVSKDF
                     YKVLKSPLVSKSEKKNLVEIIFSKEVSSNIKNFLKVLVDKDRISYLEDIELAYKELLN
                     KKNNVIDGVAISAIPMSETDIKELEVKLSNKYNKNVTIENVVDKTILGGVLVRIGNEQ
                     IDGTVKTRLDKMKEKLSEVIS"
     gene            complement(23156..23653)
                     /gene="atpF"
                     /locus_tag="ACOIAF_000189"
     CDS             complement(23156..23653)
                     /gene="atpF"
                     /locus_tag="ACOIAF_000189"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HGZ2667225.1"
                     /translation="MEKALVGITWEFVFQIVNTFIIFLLLRKLLFKPVLNIIESREND
                     IKSDLAEGEKAKNEGLALKKEYESKINFAKDEGQEIIKQATIRAEQKSDDIVNTAKKD
                     ALDIKEKANKDIEQERQKVINEIKNDISNIALLAASKVIEKDLDKSKHEELIENFIKE
                     VGEAK"
     gene            complement(23776..24036)
                     /gene="atpE"
                     /locus_tag="ACOIAF_000190"
     CDS             complement(23776..24036)
                     /gene="atpE"
                     /locus_tag="ACOIAF_000190"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HGZ2667226.1"
                     /translation="METAIVAAASAIGAGIAVATGIGAGIGQGIAAAKAAEAVGNQPE
                     AKGDITSTLLLGVAIAESSAIYGLVISIILLFVNPFFKYLGM"
     gene            complement(24105..24809)
                     /gene="atpB"
                     /locus_tag="ACOIAF_000191"
     CDS             complement(24105..24809)
                     /gene="atpB"
                     /locus_tag="ACOIAF_000191"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HGZ2667227.1"
                     /translation="MNQAHYYLIGNFKISETILVSWGIMAFLAIASYFLTRNLKKVPT
                     SKVQIFLEFAVGGLAKMVTDVMGKNTVKRMPYMIPYIGSLFLFFACSNLSPLLGLRSP
                     TTDLDTTAAWAMITFFMIYFAGVKFKGLSYFKELAQPLAIMLPINIIGELARPISLSF
                     RPFGNILGGTIIMGLFYQLLGFISSLIPGLSIPIGEILIPVPLHLYFDLFAGLLQAYI
                     FIMLTMVFISSAGELD"
     gene            complement(24812..25198)
                     /locus_tag="ACOIAF_000192"
     CDS             complement(24812..25198)
                     /locus_tag="ACOIAF_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HGZ2667228.1"
                     /translation="MDIKLLNQVRSVTKGIVIFDVVLIVLMLITSTLSKPVLLGLIFG
                     SIIAMLNFRLLAISLEKSVTFPAGKAQAYASAQYAVRLFIMAVVLIASATVPHLNIIG
                     TALGLLSTKFVILSKNFINKLKRKEA"
     gene            complement(25203..25430)
                     /locus_tag="ACOIAF_000193"
     CDS             complement(25203..25430)
                     /locus_tag="ACOIAF_000193"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HGZ2667229.1"
                     /translation="MSKRNTHTEIAKLFSLISQIGLMIVISILGCTFLGKFLDSKLNT
                     TPVLTIIFLLLGVGGAFSGVYKTLIVYTRRK"
     gene            complement(25615..26052)
                     /locus_tag="ACOIAF_000194"
     CDS             complement(25615..26052)
                     /locus_tag="ACOIAF_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HGZ2667230.1"
                     /translation="MRPSWDEYFMEIAEVVKKRSTCIRRQVGAVIVRDKQILTTGYNG
                     SPRNLEHCENIGCKRQELNIPSGERHELCRALHAEQNAIIQAAHNGISVDGATLYVTT
                     RPCVLCAKMCINAGILKIVYEGDYPDDMSTELLKEAGIELIKF"
     gene            complement(26278..27093)
                     /gene="yqeB"
                     /locus_tag="ACOIAF_000195"
     CDS             complement(26278..27093)
                     /gene="yqeB"
                     /locus_tag="ACOIAF_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HGZ2667231.1"
                     /translation="MKNCIVVRGAGDLATAVIHKLHNSGFKVVALEIEEPLAIRRQIS
                     FCEAVYEKKVTVEGVECIFCNTEKEIEEVLSQHKVALIIDPKGEKLSKLNPIVLVDGI
                     LAKKNLGTTKDMAPLTIALGPGFCAGEDVDVVIETMRGHNLGRIITNGRAEKNTGVPG
                     AIKGFSKERVMHSQYDGIMENVCKIGDIVEKGDLLCYINNYEKKHEVRATISGVLRGL
                     LKDGAKVNKNLKILDIDPRKEEVKNAFTISDKARCIAGGVLEAILNNGIYPQN"
     gene            complement(27388..28017)
                     /gene="upp"
                     /locus_tag="ACOIAF_000196"
     CDS             complement(27388..28017)
                     /gene="upp"
                     /locus_tag="ACOIAF_000196"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011971477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HGZ2667232.1"
                     /translation="MSKVVETNHPLIQHKLTLMRDKNTGSKDFRELLTEIAMLMGYEI
                     TKDIPLKDVEIETPIQKTSSKVVAGKKLAIIPILRAGLGMVDGLVSLMPAAKVGHVGL
                     YRDPETLKPVEYYCKLPQDIGERDIIVVDPMLATGGSAVAAIDLLKSKGAKSIKLANL
                     VAAPEGIAEVQKYHDDVDIYVASVDERLNEHGYIIPGLGDAGDRLFGTK"
     gene            complement(28053..28505)
                     /gene="rpiB"
                     /locus_tag="ACOIAF_000197"
     CDS             complement(28053..28505)
                     /gene="rpiB"
                     /locus_tag="ACOIAF_000197"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HGZ2667233.1"
                     /translation="MKIGLGCDHGGYNLKKEIISYLESKGIECVDYGTNNATDSVDYP
                     VYGEIVANSVINKEVDYGILCCGTGIGISLAANKVPGIRCAVVSDVFSAKMSKAHNDA
                     NMLSLGERVLGKGLALEIVEAWINTDFEGDRHARRVNMIKSIEEKHNK"
     gene            complement(28522..28971)
                     /locus_tag="ACOIAF_000198"
     CDS             complement(28522..28971)
                     /locus_tag="ACOIAF_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HGZ2667234.1"
                     /translation="MNILIVCTGNTCRSPMAEAILRKAIKESGRSIEEYSISSAGIST
                     ANGMGASENSIEVLKEIGIDLSNHRSKVITKKLIDESDIILTMTKSHKEILVQAVPKC
                     KEKVYTFKEFANKNEEDISDPFGGNLDIYRSTMREIMYSVNEIVKKI"
     gene            complement(28988..30028)
                     /locus_tag="ACOIAF_000199"
     CDS             complement(28988..30028)
                     /locus_tag="ACOIAF_000199"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HGZ2667235.1"
                     /translation="MKTIISNIDINNIDYEEIKIQAKLLREGKTVIFPTETVYGLGAN
                     ALDENAVKKIYEAKGRPSDNPLIVHIYEKEEVYDLAKDISDKAKLVIEKLWPGPITII
                     LNKKDIIPYKTSGGLDTVAIRMPSNVIARAIIKEAGIPIAAPSANISGRPSPTKAKHV
                     YEEMNGRVDGIVLGGDSNFGLESTVLDLTEETPMILRPGSITKEVLEELLGEVKLDPS
                     LSKKEDNQKAKAPGMKYKHYSPNADVYIISGKRENVATKINDMIKSNRDKGLKTGVMC
                     ISRNREFYDGEVIDLGNSLEEVASNLFDALIEMDKKGVDVIYSEEFPKTGVGQAIMNR
                     LLKSAGYKVIKA"
     gene            complement(30057..30737)
                     /locus_tag="ACOIAF_000200"
     CDS             complement(30057..30737)
                     /locus_tag="ACOIAF_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HGZ2667236.1"
                     /translation="MILKVTIIGLLAGVIGTGLGGVISAIFKREVDKYLSFFMGLSGG
                     IMLAVVVFDLMKESMDKMGIINTVIFTFVGALITMYIKTKLDVSGNMASGYLIFISIL
                     LHNLPEGLAIGSSFMSTESLGITLAIVIGLHNIPEGLAMALGLVCNKMKLSKVILFTV
                     IAGLPMGLGSFLGVYFGGVFTSLIGVFLATAGGTMMYVVLEEIFPHSKSVYCIIGFLL
                     GTMIVNYI"
     gene            complement(30866..31930)
                     /gene="prfA"
                     /locus_tag="ACOIAF_000201"
     CDS             complement(30866..31930)
                     /gene="prfA"
                     /locus_tag="ACOIAF_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HGZ2667237.1"
                     /translation="MLKKLEVLEDTYKDLSEKIGDPDVINDQKVWQKYIKEHADLEPI
                     VMKYREYKSVLDSIKESKEILQEESDEELRELAKMELAEMEEKVAPLEEEIKILLLPK
                     DPNDDKNVIVEIRGGAGGDEAALFAGDLFRMYSRYAERRRWKIELLSASDTGVGGYKE
                     VSFMIKGKGAYSRLKYESGVHRVQRIPSTESGGRIHTSTSTVAVLPEVEDVEVEINPN
                     DLRIDVFRSSGNGGQSVNTTDSAVRVTHIPTGEVVSCQDGKSQLKNKEQALKILKARL
                     YDKALAEQHKDIAAERKSQVGTGDRSERIRTYNFPQGRISDHRINLTLYKLDAFLDGD
                     IDEMIDALITVDQTEKMTAI"
     gene            complement(31974..32822)
                     /gene="prmC"
                     /locus_tag="ACOIAF_000202"
     CDS             complement(31974..32822)
                     /gene="prmC"
                     /locus_tag="ACOIAF_000202"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HGZ2667238.1"
                     /translation="MTIKDIIIKYSDKLKDISDTPRLDTELLLQKTLGVDRLYIHLNL
                     NKELTEEQKTKFMGFAEERLNGRPIAYIVENREFMGLDFFVKEGVLIPRPDTETLVEE
                     IIEICREKKDVSILDIGTGSGAITISLAKYIENSKIMSFDISETALEIAKKNAIINEV
                     GEKIKYINSDLFTAISDSNIKFDIIVSNPPYIKKQDIETLHKQVKDYEPYNALEGGED
                     GLDFYRRITEQGKKYLNKCGILAYEVGHNQAEDVINIMKSNGYKKIYTKKDIQGIDRV
                     VIGYNI"
     gene            complement(32840..33733)
                     /locus_tag="ACOIAF_000203"
     CDS             complement(32840..33733)
                     /locus_tag="ACOIAF_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HGZ2667239.1"
                     /translation="MAKQAVGGQAVIEGVMMQSKDKRAVAVRKSDGEIALKEDRIKSW
                     VRDKNIDKIPFVRGSFVMIDTMIQGIKSLNFSSEFFMEEAEEDKFDLFIKKIFKDKAN
                     DIIIIFSLVIAMLLSAGLFIFIPTLVGGAFSKVMPNDFMLNLIEGIIRIAILFAYIVL
                     ISRSKDIERVFQYHGAEHKSIYCYENDLELTVENARKFKRLHPRCGTNFLFIVMAVSI
                     ILFAFFGWPNPILRIFMRIICVPIVAGLSYEVIRVLGKYDNGFTKIIAYPGMMLQYFT
                     TKEPDDEQLEVALEALKAVVD"
     gene            complement(33878..34078)
                     /gene="rpmE"
                     /locus_tag="ACOIAF_000204"
     CDS             complement(33878..34078)
                     /gene="rpmE"
                     /locus_tag="ACOIAF_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018591942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HGZ2667240.1"
                     /translation="MQKEIQPKYNPVEVRCACGNTFVAGSTKDEIKVEICSECHPFYT
                     GKQKNIEKGGRIDKFKKRFKMD"
     gene            complement(34231..35835)
                     /gene="rho"
                     /locus_tag="ACOIAF_000205"
     CDS             complement(34231..35835)
                     /gene="rho"
                     /locus_tag="ACOIAF_000205"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HGZ2667241.1"
                     /translation="MRRIFLILDDKVLEGLGSKTLVELREIAKELKIKSITTYKKNEL
                     IEIINSKSKNEEKVNEETKEKNEDIKEDIKNRKENIERESIEKEVEYKSPTKSYNKKE
                     IDTQNISQNQEHRNQRNDYNKTTSVNDSNKSSNISNRMVRNNNKNYYMPKQVDESKIV
                     DEFNTSKEDEVVGVLEILPDGFGFLRGSNYLSTEGDVYVSPSQIRRFNMKTGDKIKGI
                     TRHPKSGEKFRALLYVQKINDENPDTAIQRNAFETLTPIFPEERLTLETNRNEIATRI
                     IDLISPIGKGQRGLIVAPPKAGKTVLLKSVANSIAKNHPNVELIVLLIDERPEEVTDM
                     KESIEGDVIYSTFDQVSSHHVKVAEMVLNRAQRLVEHGKDVVILLDSITRLARAYNLT
                     ISPTGRTLSGGIDPGALHGPKKFFGAARNIRQGGSLTILGTALVETGSRMDDVIFEEF
                     KGTGNMELHLDRKLAEKRIFPAIDIYKSGTRRDDLLLDDEEKTALWRLRREMSNNSVM
                     EITDKVIELIKRTKDNKEFVKSIKNL"
     gene            complement(36335..37300)
                     /gene="galU"
                     /locus_tag="ACOIAF_000206"
     CDS             complement(36335..37300)
                     /gene="galU"
                     /locus_tag="ACOIAF_000206"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HGZ2667242.1"
                     /translation="MQVKVKKAVIPAAGLGTRFLPATKAQPKEMLPIVDKPTLQYIIE
                     EAVASGIEEILIITGRNKKSIEDHFDKSVELELDLEKKGKKELLEVVQNISNMINIHY
                     IRQKEPKGLGDAIYCARHFIGDEPFAVMLGDDIVDNDVPCLKQLTDAYEEYRTTILGV
                     QKVNQEDTNKYGIIEAKNIEGRVYKVKDMVEKPESGKAPSNIAILGRYIITPEIFDIL
                     KDLPPGKGGEVQLTDALKILSKKEAMYAYNFEGKRYDVGDKLGFLEATVDFALKKEDL
                     KEDFIKYLKHVCSEFDKNNNVLYNNTDEKIDLIEVNEARELEIQK"
     gene            complement(37414..39306)
                     /gene="pepF"
                     /locus_tag="ACOIAF_000207"
     CDS             complement(37414..39306)
                     /gene="pepF"
                     /locus_tag="ACOIAF_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HGZ2667243.1"
                     /translation="MNRKLCKAIIPLFISIFCMLIISIKADQGYQKNNDRAITVMNNN
                     DTEDDAEKKQEIDYEKTCWNLQSLFKSDDQWKKELKSFDKDTKELKNYIGKVTKSPTH
                     LSFALNIKEKLDIRLNKLSAYVKLKQDTNKNSYKYLDMNDSMSKSYGNYMGICSDLEL
                     EILKLSNKDYKKIISNKNINRKYGAYLRDIRRNKVHYLDDKSEDILSKVSSISSLPSQ
                     VYELFRNMDRKTNLTPAQYASELESSDRENRKKAYQDELITYNDNINTIAGLITGQVK
                     KNIFYSQERGYESSLEMYLEADNVDEKVYNNLIRTVNSNMDSLHKYIDLRKKVLKLDK
                     VHSYDMFVPMVKAVDNNINYEKAQSIVYSALSPLGKEYNDVVYKAFNEKWIDVYSHEN
                     KVSGGYCLSVYENHPYILLNYNNSLGSVSTLSHELGHAVYEYLSSKNQNYFNSNPSIF
                     THEVASTTNEALLYEMLIKEAKGNDEKAYYITQYLDLIKDTLYTQTMYAEFEKTIHEL
                     VEKGKSVNALVLNDVWGQLLKKYYGQSYELDQLSKVGWARIPHFYNSFYVYKYATGCS
                     AGVSLAQDILKNGSDNYMSFLKKGSSDYPINLLKSSGINLTNTKPIENTIKKFDSLVA
                     ELEKLL"
     gene            complement(39657..42029)
                     /gene="spoIIE"
                     /locus_tag="ACOIAF_000208"
     CDS             complement(39657..42029)
                     /gene="spoIIE"
                     /locus_tag="ACOIAF_000208"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein E"
                     /protein_id="HGZ2667244.1"
                     /translation="MQRSVAVVKNKTVKPNVIKLISGYIDFTTAAFAVMGFLLSRSII
                     VDSVAPLGIAFFICASKIDKYKIPVFISTLLGILLSSNDFGNVAKYAVCLTIFMAMNK
                     KLKLSDSIAKVAFIGAIILLPISMGQAFFSNRYVYGLLIAGVEAIIMFISIYVFSYGI
                     GLMANVNNRMSVRTEEAISISLLLTFSIMGIGDISLFGISIRTILSTVLILLTSIVGG
                     ATMGASSGVIIGVASILNNMTSAVYMGIYSFSGLISGAFNKINKYFCILGYILSWTII
                     YLYTSGITSNMMQLRDILLGCLIVLVLPERLFNKIEKLIKSNVASNEIVYDYIMRSKN
                     LTNSRLNSIYKTYDDLADTFDKIREKDKVLDQRDIANVIDMIHNDECKSCSMRRMCWE
                     SRFNHTYTMIYNILEKIEEKGELSLNDIPKNFRKECMKPESIVKISNHYYKMFVLDYD
                     WSVKFSESRKLIANQIRSISKSIKSLSQDLEGDIMLDIEKEKNIYEQLERYDITVDKV
                     SYLTKSNSEFEISIEKKTCHDGCMCEDKIVNIISDLVGENMSVRKIGCHCLGGKCKAT
                     FVKSQKYKAVTEVSAMSRDGHILCGDNYTYMEINDGKYMMAISDGMGKGKKAYEESSA
                     TIDILEKMIDAKIKDEIVIDTINNMLLLKSSEEMFSTLDLGILDLKRGCLETIKMGAC
                     STYIKREDGEVDLISSSSLPVGILSDVKIDRKNVKVKEGDYVIMVSDGIVDAGRNNNL
                     GDNWLIYFLKNIETTNPKEISNLILDRALELQALQIEDDMTVLVTKICTK"
     gene            complement(42119..43039)
                     /locus_tag="ACOIAF_000209"
     CDS             complement(42119..43039)
                     /locus_tag="ACOIAF_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HGZ2667245.1"
                     /translation="MKVGAIDIGTNSMRLLIADYRDGNFINRKKFVNTTRIGYGVDSK
                     GYIKEDAIKRNIKALKEFSDKCNDEKCEKIYCMGTSALRDSKNGDYFVKLAKEETGID
                     VDIVTGIEESNLGFKGVLEGIQGDDTIIVIDIGGGSTEFIVGDSEGIKFNKSENVGAL
                     RMTERFLKHDIIEDEEFEAMSSFIYDEIDKTLNHIKDRGLKTLIGIGGTITSLSAMNQ
                     ELEIYSMEKIHNSKIHKKEIEEILQKLIKMTLSDKKNLKGLQEKRADIITAGVKILNI
                     IMEKLEIEEITVSEYDNLEGLICQKSKKMY"
     gene            complement(43152..43436)
                     /locus_tag="ACOIAF_000210"
     CDS             complement(43152..43436)
                     /locus_tag="ACOIAF_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HGZ2667246.1"
                     /translation="MNLRKKFSGQIIVISLFLGISIFSMMTGFVFEYTKAKEYKKEIA
                     SLNKQLKKTEIQINSLKKDEKSYEGDLEDIARKRLNMVKPNETVYVDINR"
     gene            complement(43442..43975)
                     /gene="yabQ"
                     /locus_tag="ACOIAF_000211"
     CDS             complement(43442..43975)
                     /gene="yabQ"
                     /locus_tag="ACOIAF_000211"
                     /inference="COORDINATES: protein motif:HMM:TIGR02893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0034301 - endospore formation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex biosynthesis protein YabQ"
                     /protein_id="HGZ2667247.1"
                     /translation="MIPFTQDVSIFYATIYGGILIGVLFDFYRGLRGNFKFINYFAII
                     FDVLFWFLATVIIFVTINLTEFFDLRYYHFVALFIGFILYYNTISKIVLSIINKIIRF
                     VRNSFKKVTHYIVSFLNNLYYVIIYSLHLLFDIIFYIPNIFIATRKSIKRKSNKKLKN
                     KKKSKPKKKKNKTKKRV"
     gene            complement(43976..44230)
                     /locus_tag="ACOIAF_000212"
     CDS             complement(43976..44230)
                     /locus_tag="ACOIAF_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YabP/YqfC family sporulation protein"
                     /protein_id="HGZ2667248.1"
                     /translation="MEQNITLKDRSKLVISGVEHIYSFNDKRVELKTSVGEMVIEGEN
                     LDMSKLSIDENIISIDGTINSMVYAKPKKPQESFLKKVFR"
     gene            complement(44292..44534)
                     /locus_tag="ACOIAF_000213"
     CDS             complement(44292..44534)
                     /locus_tag="ACOIAF_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HGZ2667249.1"
                     /translation="MRLDKFLKVSRIIKRRTVAKDACDKGIVTINGKVAKSSSEVNIG
                     DTLEIQFGEKKMKFKINEVKEHVLKNDAKEMYEILE"
     gene            complement(44606..44884)
                     /locus_tag="ACOIAF_000214"
     CDS             complement(44606..44884)
                     /locus_tag="ACOIAF_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HGZ2667250.1"
                     /translation="MNKAELVSKMAEKSGLTKKEAEAALNAFMSSVQDALVNNEKVQL
                     VGFGTFETRERAARQGRNPRDPEQVIDIPASKAPVFKAGKGLKDIING"
     gene            complement(44973..46421)
                     /gene="mazG"
                     /locus_tag="ACOIAF_000215"
     CDS             complement(44973..46421)
                     /gene="mazG"
                     /locus_tag="ACOIAF_000215"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HGZ2667251.1"
                     /translation="MGKISIVGLGPGDYSLISQGVLESLINSPKVFFRTEKHPIIDKL
                     KETIQYTSLDYFYEKEENFDNVYLKISEFIIEESAKGDLVYAVPGHPRVAEKTVSIIE
                     RLANEKHIAVEILPSMSFVDAMFNYLAIDPSEGFKLVDAFEIENSYIDLDTSMIITQV
                     YDKFIASNVKLKLMEHYDYNQEVCIVNGAGVKDLESKKFIKLCELDRNENFFDYLTSL
                     YIPKSSKKMYNTVHDLEIIVNTLRSPSGCEWDKKQTHQSLKNSVIEEAYELCNAIDNN
                     DIDEMVEELGDVLLQVIFHCQIGNEEGYFDLKEVVNGICKKLIHRHPHIFSGAKLDMS
                     KFEKTWEELKKEEKGESTITDGLRRIPKHLPALMKASKVQHKAALVGFDWDNVEDVFK
                     KIEEEYQELLDEHRVGNIKYIKEELGDLLFSIVNLARFLDIDSGEALNCTTEKFINRF
                     DFIEKSAMNLNKKLEDMTLEEMDEFWNQAKKQ"
     gene            complement(46528..48141)
                     /locus_tag="ACOIAF_000216"
     CDS             complement(46528..48141)
                     /locus_tag="ACOIAF_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HGZ2667252.1"
                     /translation="MNNNKNKDSFLKGALILGLAGVIVKIMGGFFRIPLGNMIGSEGM
                     GYYQAAYPVYTLFLTLATAGFPTAVAKLVSEKVAIGNFKGANKIFKVSHTVLFITGII
                     SFCILFFGADYIVTNVMKNPGALYSMKAIAPALLFVPAMSAYRGYFQGRQDMTKIAVS
                     QVAEQFFRVVLGLTLAYLLMKSLGQTFGAAGAISGATIGSIASMLYLVFAYMLGRKER
                     RAEIDASQRFKDERVSYIFKKLLTVAIPITIGASVMPLVNMIDNVIVIRRLMEAGFTY
                     KVANSMFGQLTGMAMATINLPAVITTAMSMSLVPAISKAYALGNKSKARKDTKSAVKV
                     TLLIVLPCAFGMASLAIPIMGLLFPHEPSSVGTILFTLTPCVLFLGLIQTLTGIIQGM
                     GKPIVPVIALCVGMLCKIVISYTLTGIPDINVLGSAFGTVTAYFVAAMINLLYVKKHM
                     NVNFSKKEFIIKPFITVMTMFIMVKLSYGALVGFLGNSISTIIAICVGGIVYVVVILG
                     IGGIKKEEILTMPKGDKLYKLLKKVKLIR"
     gene            complement(48261..48800)
                     /gene="spoVT"
                     /locus_tag="ACOIAF_000217"
     CDS             complement(48261..48800)
                     /gene="spoVT"
                     /locus_tag="ACOIAF_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein T"
                     /protein_id="HGZ2667253.1"
                     /translation="MKATGIVRRIDDLGRVVIPKEIRKTLRIREGDPLEIFTAKDGEV
                     ILKKYSPIGELNEFSQEYAETLAEVLGYGVLITDLDSIIAVSKLPKKDYKEKSISDEL
                     EAIISERTVKYSKDKNILPLFKEDQKEYTSQVIMPIISASGDCIGSIVVVSKDKDTIA
                     ESEEKSLKIAANFLGKQVQ"
     gene            complement(48924..49919)
                     /locus_tag="ACOIAF_000218"
     CDS             complement(48924..49919)
                     /locus_tag="ACOIAF_000218"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HGZ2667254.1"
                     /translation="MKKVITLVIAMILVVSVTACSSSKGETVATVEGTKISSDEFKKT
                     IALYKDSMEQTYGKDIWDKEVEKGVKYKDKFKDLILDQLITTEVIYSQAKKDNLLPKK
                     EDVEKSFKELKDAMGKDEKYKEQLKKLGIDDEFLRDQQEKDLAMQNYQSNFAKKTKIS
                     DEEMKKYYDTHKDEFKKDEVEASHILLKTVDDNNKPLSDKEKAEAKKKAEEALKEVKS
                     GEDFAKVAKKYSQDTSASDGGKLGFFSRGQMVAEFEDAAFSMKKGEVSDLVETQYGYH
                     IIKVTDRINEQTSFEDAKETIKDQLLKNKYQEQIEKLTKEAKVEKDEKVINKITI"
     gene            complement(49950..53336)
                     /gene="mfd"
                     /locus_tag="ACOIAF_000219"
     CDS             complement(49950..53336)
                     /gene="mfd"
                     /locus_tag="ACOIAF_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HGZ2667255.1"
                     /translation="MNDVFLYPLQNSKEYKDIINCIKNTKGSLLVNGLLPVQKPHISY
                     SIFNDLSRQMIFITSSDLEAKKVYEDLSFYMEDKVEYLGFQDIYFYHLDAKDRNEEAK
                     KLKVLLKLANKEKIILVTSIEAVLRKYIPKQVLLDSVSHYKVGDSLDLEKLTEKLVSL
                     GYERVSKIEGFGQFSIRGGIIDVFSLEYTNPIRMELFDDEIDSIRTFDVYSQKSIDKL
                     QQFSITPSREFIYPEKTTDALVKLKKETTKNTDEGTFQNIDYISSKTYFEGVENYIDY
                     IYPEENKSIFTYLADDAIVFINDITRLKERCENYINEFRENYKLNLERGLAIKNQGKL
                     LYHYTDLEYLVKDKSVVLNSLLPKSINNFSIKSIINFESREVPTFNGKVDLLVEELNR
                     LKYNGYKIILATNTLERANKLGKDLLDKGLETTISKDRDIEIKSSQVIIVPAHINSGF
                     QYKSIKFVVITDNEMIGVYKRASKTSNKKVKKGKKIESFLDLSVGDYVVHENSGVGRY
                     TGIEQITVNAIKKDYMKIVYQGGDNLYVPIDQMDKVQKYIGAEVEKVKLNKLGTNEWT
                     KAKAKVKKEIEDMTKDLIELYAKREKIQGYKFSKDTPWQAEFESLFPYQETEDQLKAI
                     EETKKDMESSKVMDRLVCGDVGYGKTEVAIRSIFKACMDQKQVAVLVPTTILAQQHYN
                     TFKERFENYPLRVEVLSRFKTPKQQKQIIEDAKKGLVDILIGTHRIISKDINLPNLGL
                     VVIDEEQRFGVKHKEALKKIKSTVDVLTLSATPIPRTLHMSLSGIRDMSVIEEPPQER
                     HPVITYVTESKESVIQDEIERELSRGGQVFFVYNRVEHIEEMASMIQKLVPDARVAVA
                     HGRMTSKSLENIILGFLNKDYDVLVCTTIIETGMDISNANTMIIYDADKMGLAQLYQL
                     RGRVGRSSRQGYAYLLYEKDKTLSEIAEKRLKAIREFTEFGSGFKIAMRDLEIRGAGN
                     ILGSQQHGHMAVIGYDLYVKMLNDAIKKVKGEPIVEEIDVEIDLSVNAYIPDNYIKDE
                     LIKIEMYKKIASIENKEDMLDIQEELEDRFSDIPKPVQTLLTIAYIKSLCKILKIEKI
                     RQLKDEILLVPITKYRTKQKIGYNIVTELEELLEKMCKVK"
     gene            complement(53353..53913)
                     /gene="pth"
                     /locus_tag="ACOIAF_000220"
     CDS             complement(53353..53913)
                     /gene="pth"
                     /locus_tag="ACOIAF_000220"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HGZ2667256.1"
                     /translation="MYVVVGLGNPGKKYEKTRHNVGFDVIDILAKEYNISVTKIKHKA
                     LIGEGRVGTEKVLLVKPQTYMNLSGETLIDIYKYYKVDLSNIVVVYDDIDLEVGKIRI
                     RKKGSGGTHNGMKSITKCLGSNDFPRVRVGVSKPEAGQDLADFVLSRFRKEESDNINE
                     ALEKAADAIDSIIRENIDMSMNKYNG"
     gene            complement(53927..54577)
                     /locus_tag="ACOIAF_000221"
     CDS             complement(53927..54577)
                     /locus_tag="ACOIAF_000221"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HGZ2667257.1"
                     /translation="MEYIIIISLFFAHLINKSLCVFCDREKKNLYNTFFIYIITVVLS
                     VLIYLKFGISVECVQYMVLIPFLIIISIVDYYTIYVYDITIVSGIIVQGIIFLVFFNI
                     VQFDYINHIFGLLIGFIIPYIIAKLTKGLGYGDVGLYSLCCFALGNNYGFYVIVLSFI
                     LAFLFFIILYITKIFKDVDIRKQIPFTPFISLSTILIIFTEYNIFNFYYNLLYKFI"
     gene            complement(54679..55461)
                     /locus_tag="ACOIAF_000222"
     CDS             complement(54679..55461)
                     /locus_tag="ACOIAF_000222"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HGZ2667258.1"
                     /translation="MDIIIVNLYVFIVGIVFGSFFNVCIFRIPEGISISRPPSHCMSC
                     NTRLKPIDLIPVLSYLMSGRKCRYCYEKISSRYAIVELITGLLFLTVFMLYGISTSTI
                     YYLVLVSLLIIITFIDIDHFIIPDKILVFGAIFSMIFNLLFKEIPVKDSILGVFICGG
                     SVWIIVLLIEFVIKKECMGGGDIKLFAMLGFYMGVKNGLLTALLSVYVGAVYGICVII
                     ISRIKRKEYNSVIPYGPFISIGALITILCGKQLLELYFSMVI"
     gene            complement(55442..56524)
                     /locus_tag="ACOIAF_000223"
     CDS             complement(55442..56524)
                     /locus_tag="ACOIAF_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HGZ2667259.1"
                     /translation="MYIYDLLEQGIRLNASDIHITVGTNPVARVKGGFVKLSEQILTS
                     EVTMQMAKDIAGESMFKVIEEHGEADFSASLKTGERFRVNAYRQKGNYAIAIRTITAE
                     IPTFEKLGLPESIKSFTEKHKGLVLVTGPTGSGKSTTLASMINIINEKQQKHIITLED
                     PIEYVHHHKQSLVNQREVGTDTESFHSALRAILRQDPDVILIGEMRDPETVSIALTAA
                     ETGHLVFSTLHTVGAAKTIDRIVDMFQPSQQQQIKTQLSTVCEGVVSQQLLPTADGKG
                     RIAAIELMFATPAIKNLIREGKTYQIPNMIQTGVKVGMKTMDQDLMELYKNGKITKDM
                     ALSRCTDQEFMTRMIGGVNYNGYYNR"
     gene            complement(56624..58162)
                     /locus_tag="ACOIAF_000224"
     CDS             complement(56624..58162)
                     /locus_tag="ACOIAF_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667260.1"
                     /translation="MRKWNKFKSERGAALVLVLVVVALLSIVGLIFSNQIANRIKSTK
                     TTNEGIQAKYLAETCVENSIDKAYEKLYDELEKMDNEFKSENQEKSISRSKLRNISDE
                     DFNNQDEENIEAERLGYMNNINFYLNKASSDLEKASMELKKLYDLDMLDYRDIEYVDA
                     NIISHRDSILEICKNYTSGDISKINEYILKEDIDSTTLIEAKLVNNDILLKMFLEENK
                     IENEHLNSAFSHTYKALDNISLAMQNMIEYRHTFHIDEPKVEVSNGIPDSQQYYELIQ
                     NPIINSMEYSWNSKWDTLENLLEILPDQTQGFNSLRVHLRNNVRKFEKLSDNISSGKK
                     NTAKNFLKYKELLYEISDQCNQLKSMSYEKIPVKYDNMALITTFDYIQNELLAEIKCR
                     LKELKPQEIDKTEGITIKIPFYKADYDMTKEGWPKLKENGSGAELSLMVTGDKDGIKE
                     VEVTDGKKNIIGLGVEENSNSKYKVDAIVNFNLNIDTNVVGNYDIKDKILINHDISSY
                     KKVN"
     gene            complement(58183..58710)
                     /locus_tag="ACOIAF_000225"
     CDS             complement(58183..58710)
                     /locus_tag="ACOIAF_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HGZ2667261.1"
                     /translation="MSKKNSERGFSLIEVLVAMAIMGIVLFAFFNIINTNNKANTKND
                     TDITSLNYVQSEIENLREKIKSGEFDFDSLDKLEDGTVVYEKLIDKSKKVVYDKVLSE
                     GDVSLYDTPYEKITTIKDEDGNLIDKGNITNKIKTIVEDKSGQIYKIAVTGKSMNDYS
                     SKKEVKIVTEIFKDK"
     gene            complement(58747..59313)
                     /locus_tag="ACOIAF_000226"
     CDS             complement(58747..59313)
                     /locus_tag="ACOIAF_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HGZ2667262.1"
                     /translation="MSLYKNNEKGLTLLEVIIAVFILTIVLSISYKVFNGITSAVKKQ
                     QIITDAQVNINLINKYLNRDLENCKELTKTGSGNNYEYNIEMPDNVVKYEVSIETKKN
                     TEVYSVTRIQNNTIDTENEVREEIIYNQPLVQNNKEMKETPFKIEKQTGKSIYTVSIY
                     YNESVQESHKNSNLNNKTYTFDVMSRIG"
     gene            complement(59340..60248)
                     /locus_tag="ACOIAF_000227"
     CDS             complement(59340..60248)
                     /locus_tag="ACOIAF_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HGZ2667263.1"
                     /translation="MNKDIFKKDIKDIFKMEVSLKGILIKVIILLAILSYGYFGLYPQ
                     YQKYIATKNSVKQDTEKLNIYKEKTALMPKYEKQLEEVESELKEKSKNLLYDMQDGLF
                     LIGLDKKMKAYNIDLTSYDVGNVKEYEHFYGISTTISVRGDYRNVKKLMAYLEEQKNV
                     TQILDYTMTSYIEPEKEEEETTNNKDLIKNVNINKDTKIYWVEGENKWHLYKDCPLIK
                     EGEILKNDKLLDRSTNYLCNTCVLRAATEKEAKKEVVAPKAEGVVDATFKFIMYTKEN
                     TTMILDTDNPSSWRPGKYNPFKSSAK"
     gene            complement(60241..61953)
                     /locus_tag="ACOIAF_000228"
     CDS             complement(60241..61953)
                     /locus_tag="ACOIAF_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HGZ2667264.1"
                     /translation="MNKVYVSYYGDSISIVEGRYKHDKDKFYVKNFNVFSIEDIAQDF
                     SKEKYALLRYALENVKLKSRHVVFCLNTRDVILKPQKLPNMDKKDLDGFMKLEMDEMM
                     ALESDEYVFSYEVSSETPDNSDGDESSLNLIIGAIQKEEVNAIVEIMHEFDLVLDRID
                     TLSTAYLRMLKNLDYNDIMVTNISDNSTIINIYKKDTLFISDNIPIKITAKDMDMQLL
                     NVVEESKGLMNYYSSRNFGKVTDTILILGKRYSNKLIYETFSSAFSSYVSQGLTEIMD
                     ISDMITGDIDEDDINSIVELLGSMIEPKGKRDFENINFLPMDILRKQIKAKQMKNILK
                     IAPIVILILAIPYLCLIFSNNLANKELENVNGEIRNIESAYYQIGNIEKMIQSKSEEI
                     KVYDMLIGKKVKWENLLDDIDKNTPSKVELTSLSTTYQNLGTNNKADNSKSNKTTTDK
                     SSNNETKSSTKSNSNGDKTDNDLNEDKTTNVSEALTGNSETNSNNTDKDDKTPLYDKI
                     PNVINIEGNAKDSSYVGQFLYKLKGLYYFKDVKLHSIKSNAEDSNYTFSMTLYLKEGV
                     LTNE"
     gene            complement(61968..63176)
                     /locus_tag="ACOIAF_000229"
     CDS             complement(61968..63176)
                     /locus_tag="ACOIAF_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HGZ2667265.1"
                     /translation="MKSFKYTVIKEDGKKQTDVIEANDFNEARNLLRKRNLRVIEIKE
                     SKNKNIRLSSRKRKKDLGADQISHFCRQFAIIVSSGINSISGLETLARRSTNITLREE
                     INRIVAEIKVGSTIADSMLSSKSKFPKLLGAMVATGEATGKLDEVLKSMASFYASEHR
                     VKQKLRNAATYPLIVLISSFIMIFIFTTFMVPKMINSITTVGATLPLITRIVMGFGMF
                     MKKFWIIVLLVIILFIYQFKKYLKTSVGRAHKDRVINKIPVLGKGINCMVATRFSRAL
                     YLFVSTGYPLVQGLDYIIDSVNNTMAEKILASAKDGITRGEGLAENLERYTYFDSVLV
                     QMIAIGEQTGELENISRQMAEFYEYESEIYLNRMASMIEPVLIIAVGIIVAILVVSIF
                     MPMLSIYDAM"
     gene            complement(63188..64864)
                     /locus_tag="ACOIAF_000230"
     CDS             complement(63188..64864)
                     /locus_tag="ACOIAF_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HGZ2667266.1"
                     /translation="MAKKVRIGDKLVEKGYITEEQLKWALSEQKNSGKRLGEFLVQEG
                     LIDSNLLISVLKELLDIESIFLEGTEIDTLATKMVPENICKRYTVFPFKIDGNKICLA
                     MSDPQDREAVQDVRRMSGKDVEIFISSTEDINKAIGHAYAHSEINKAMTEYNKNRTGG
                     VRETVILEEDVNAAPIVRLVNNILENAVRMEASDIHIEQSENYMRVRFRIDGMLREYM
                     RMNSAPYKAVISRIKIMSDINISEKRIPQDGRIYLKVDNKPIDFRVSTMPTNRDEKIA
                     MRVLDKSNFMVSKEVLGIDEHGSKIYDELINTPYGLILVVGPTGSGKTTTLYSMLNQL
                     NTENRNLLTIEDPIEYELPGVNQSQINEKAGLTFASGLRAFMRQDPDIIMVGEIRDTE
                     TAEIAIRASLTGHLVLSTLHANTAVGAISRLLDMDVESFLITSSVLGVISQRLTRKIC
                     EHCKVSYEADIGEKKALGIDVNESVTIYRGKGCERCNNTGYKGRLGIFEMLEITPEIK
                     ELIDSSANQREILKMARKQGMVSLKEDIVKKVLNGKTTVEEMIRIILMTD"
     gene            complement(65015..65533)
                     /locus_tag="ACOIAF_000231"
     CDS             complement(65015..65533)
                     /locus_tag="ACOIAF_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HGZ2667267.1"
                     /translation="MKLKKNKKGFTLVELLVVIAIIGILAVVAVPALFSNINKAKVAS
                     VESDYSSVKSAALSYYSDTNKMPATTSASDLSELKTYMDTLPDKADIGGKYQLLLVDK
                     KLVLQIGDKDGGATLTKAQSAKLLSDLGENKIYKNADLKDENELNKDDEINNKNLYIV
                     LIDNADMDSATA"
     regulatory      complement(65695..65780)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            complement(66618..67568)
                     /locus_tag="ACOIAF_000232"
     CDS             complement(66618..67568)
                     /locus_tag="ACOIAF_000232"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0002189 - ribose phosphate
                     diphosphokinase complex [Evidence IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HGZ2667268.1"
                     /translation="MNTSGSEIKIIAGNSSKELAQKIADYIGVSVLDCEVGTFSDGEI
                     CVNMNETVRGCDVFVVQSTNSPVNDNLMELLILIDALKRASAGRITAVIPYYGYARQD
                     RKAKARDPITAKLVANLITAAGADRVLTMDLHAAQIQGYFDIPLDHLLGGTILANYFN
                     EKKIEDLVVVSPDLGSVTRSRKFANTLNGEVPIAIIDKRRPKANVCEVMNLIGDVKGK
                     NVILLDDMIDTAGTIVNAANALKEFGAKDVYACCTHGVLSGPAIERIANSEISELIVL
                     DTIQLPEEKRIDKIKIKTVAPLFGDAIRMIFSNESVSKLF"
     gene            complement(67663..69042)
                     /gene="glmU"
                     /locus_tag="ACOIAF_000233"
     CDS             complement(67663..69042)
                     /gene="glmU"
                     /locus_tag="ACOIAF_000233"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HGZ2667269.1"
                     /translation="MNFKAIILAAGKGTRMKSKYPKVIHKVCGKEMVNHIIDVSKKSG
                     VKDTVVILGHEAEVVKEKLAEEIIIAMQTEQLGTGHAVKMAKEYINDEDTIVVLCGDT
                     PLIKEETLKRLFEYHIENKYHATVLTTRVGNPTGYGRIIRDKKGDLLKIVEQKDANSE
                     EKMISEINSGIYCFNGKSLREALDLLNNNNSQGEYYLTDTAKIMRDKGLKVGAFAGST
                     IEELMGVNSRVELSKAEEIMRRRINESHMVNGVTIIDTNSTYIESDVMIGNDTIIYPG
                     VMLQGKTRIGSDCIIGMNSSITNSEIGDGTEIKNSTIIDSKVGENSTVGPYAYLRPKS
                     DLGNNVKIGDFVEVKNAIIEDGSKASHLSYIGDAHVGKNVNIGCGVVFVNYDGKNKFK
                     SIVKDNAFIGSNSNLVAPVVVEEKGYIATGSTITHDVPDGALAIARERQVIKEGWVEK
                     KNQKDDQSK"
     gene            complement(69255..69533)
                     /gene="spoVG"
                     /locus_tag="ACOIAF_000234"
     CDS             complement(69255..69533)
                     /gene="spoVG"
                     /locus_tag="ACOIAF_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HGZ2667270.1"
                     /translation="MKITDVRVRKLTEEGKMKCIVSITFDNLFVVHDIKVIEGHNGLF
                     IAMPSRKVGEGNFRDIAHPINAEMRQVLEDAVLQAYHEALVQWEVAAE"
     gene            complement(69689..70567)
                     /gene="purR"
                     /locus_tag="ACOIAF_000235"
     CDS             complement(69689..70567)
                     /gene="purR"
                     /locus_tag="ACOIAF_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HGZ2667271.1"
                     /translation="MKFKRTERIGAIVKILSDNPNKIYTLSYFTNQFNAAKSTISEDL
                     LVVKNVFEKLHLGKVITISGAAGGVKYIPKTSIAENAEFLMELCERICDKSRILSGGF
                     LYLIDLIYDPTIAAKIGKIFASNIEYVDADYVVTMETKGIPMALMTAKAMNLPLVIIR
                     KDIKVSEGPTLSMTYVSGNSSKVESMSLPRKALKPDSKVIIIDDFMRGGGTIKGMVDL
                     MNEFGAEVIGTGVFISTTNPSEKMVKDYISLIQLDVNGDKIVVEPNLKTFKDEYRNED
                     LDEEEDSEFEFEIDED"
     gene            70786..72138
                     /gene="murC"
                     /locus_tag="ACOIAF_000236"
     CDS             70786..72138
                     /gene="murC"
                     /locus_tag="ACOIAF_000236"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HGZ2667272.1"
                     /translation="MNIHFIGIGGISMSALAEICINKGYQVSGSDSNESYLLDKLRDQ
                     GATIYIGQKKDNISDDVNMVVYTAAVHPDNEELVAAKEKNKLVMNRATFLGQIMREYK
                     NSIAVSGTHGKTSTTSMLSTIFEYADLDPTILVGGNLSMIGGNVKIGNSNHFITEACE
                     YVDSFLNFNPKISIVLNVEEDHLDYFSGIDEIKASFNKFGKLLPPEGYFIINGDDENI
                     DDILYDVKATIIKYGRDSDNDAVIKDIHFDNSGHGIFRIEYEGRDLGEFELSVYGLHN
                     IYNASSAIMAALVSGIDLETIRKNIKIYKGVGRRFETKGYYKNALVVDDYAHHPTELK
                     ATLAAAKKLKKSTLWCIFQPHTYTRTKSLLGEFSEAFYAADKVIITDIYAAREKDPGD
                     IHSKDLVEKLYQNNVDAIYIKEFEDIVKYLRENVKDNDLVITAGAGPIYKVADLLVEK
                     "
     gene            complement(72193..72990)
                     /locus_tag="ACOIAF_000237"
     CDS             complement(72193..72990)
                     /locus_tag="ACOIAF_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667273.1"
                     /translation="MSKKRKLKKKAAILIGVVVFFIIYFISFRIGINMEKDKSEVPKK
                     VEKEEEDNRSLRTQIKEKKKVYISDKNIENIKIEEDMWDEIKLFFSDFKKVRNTTSSY
                     EAVYEGYSDDGVKFSTDLNFFRVYTVNKEEYYKVPVSSKDAFKKLLDESIYTSFDLLS
                     HYKDWKEVTITHGKEIKKVHRWKFDDLAHKMISKRVVGKVQPEKSKERSDYNFTINIK
                     GDNFDVKVDTMGKDYVKVTSKKNVSYYEVHTSLFDYLKNEIFEIGKK"
     gene            complement(73060..73956)
                     /locus_tag="ACOIAF_000238"
     CDS             complement(73060..73956)
                     /locus_tag="ACOIAF_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HGZ2667274.1"
                     /translation="METRYEEANKITIQSILWNVVLTIIKVIAGVIGNSSAMIADGLH
                     SASDIISSIGVLIGNYVSSRPGDREHNYGHEKAETLVSFVLSILLIFVSITIGIEAIK
                     SLFNLDALSVPSILPLVVSVISILIKEYQYRITIKVAKKINSPALKADAWHHRSDALS
                     SVAAFIGIGGSILGFKPLDPIASVVVAIFVAKVGISILISSVNELMDVSVDEEEIKEL
                     KFIVADTEGVKNLGDIKTRKHGAMAYVDLTICVDENLTVKQGHDIATKLEKHIIKHME
                     FVKGITVHVEPCTNCQGNKCNN"
     gene            complement(74142..75257)
                     /locus_tag="ACOIAF_000239"
     CDS             complement(74142..75257)
                     /locus_tag="ACOIAF_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HGZ2667275.1"
                     /translation="MINEQRILNEFLELVQIDSLSLKEGNVAKVLVKKLEEIGCSVVI
                     DNAGEKANGETGNIIATLKGNKEGKKILFSSHMDTVTPGIGVKPIVDEANGIIKSDGT
                     TILGSDDKAGIAAILEGLRYINENNIEHTDIQVVFSICEECGLVGAKNLDYGKIDSEY
                     AFILDSGGSPGEIIVKAPAQDVINVKILGKTAHAGLEPEAGISAIMVAARAIENMNLL
                     RIDEETTANIGIINGGTATNIVTGEVNIIAEARSLKENKLDVQTKHMVETFEKAAKDF
                     GAQIEIDVNRAYTPIDVSEDSEIIKLAKKAFSNLGIEGHTESTGGGSDTNILSKNGIE
                     AITLGIGMKNAHTLSEHIAIKDLYDSAKMVVEIIKEA"
     gene            complement(75408..76667)
                     /locus_tag="ACOIAF_000240"
     CDS             complement(75408..76667)
                     /locus_tag="ACOIAF_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667276.1"
                     /translation="MGSKRKFKRDYIILEAKDINFRYKERVLPKAFAKVEVNDEKAII
                     ALYVENLKYVKDGYKAVAIKDDHGLIDLGNIILNEQGRGEFVLDLEDYDDEIKGIALL
                     YERNIPLIGFKGNKLENYEDILFQEVEEEEYEEYEELEEEDEEIEENEESDRDEDSEE
                     YIIEEYDEDEYEDYWEDDEDIEEGELKARTEVEDSDDEEIEEFNNYKEIENYNRVKVQ
                     EEYIDIEYDEEEEEEEVFEVNREEDRFVSSKPTKSKKVQKDQNNIEKIKRANGVSQGE
                     SGNKQTSTLLMPRQIKKGLKLFKEVKPFVTDYIDKTRWWKIEINPTTLCGYTMPYLGY
                     LNVLNYTMYSDAVLHSYKYRHYLFGVQYDEYNKRKHYIYAIPGNRNEQPDNGSTGFLR
                     YQACDNRTNSLGYWLCFIDSRQRNIVR"
     gene            complement(76733..77602)
                     /gene="rsmA"
                     /locus_tag="ACOIAF_000241"
     CDS             complement(76733..77602)
                     /gene="rsmA"
                     /locus_tag="ACOIAF_000241"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HGZ2667277.1"
                     /translation="MDRLSSHNATKEVVQKYNFKFSKSLGQNFLIDSNIIDKILSGAR
                     ITRGDNIIEVGPGIGTLTREMGKIAEKVVAIEIDRNLIPILKDTLSDLDNTEVVNQDI
                     LKVDIQELVKDKLNGGPVKLVANLPYYITTPIVMKFLEEDIPVTDIVVMVQKEVADRM
                     NAIPGTKDYGALSIAVQYYCDTEIVAKAPRHMFIPQPNVDSTVIGLHVRDKRKYDVHN
                     EDIFFKTVKASFGQRRKTLLNSLGGLGFLNKDEIREVLKEANIDEKRRGETLSIEEFS
                     VLSNIINTKVSSK"
     gene            complement(77773..78303)
                     /gene="rnmV"
                     /locus_tag="ACOIAF_000242"
     CDS             complement(77773..78303)
                     /gene="rnmV"
                     /locus_tag="ACOIAF_000242"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HGZ2667278.1"
                     /translation="MIKEIIVVEGRDDVTAVKRAIDAELITTGGFGFPKGVMERIKAA
                     NERRGVIIFTDPDFAGEKIRKKIASEVPGCKHAFLPREEAKKDGDIGIENATPQSIIA
                     ALNKVRTENTEKRDEFKQVDLIRNGLIGNEEASLRRDALGKILGIGYGNAKQFLNRLN
                     NYGVEREEFIDALKQI"
     gene            complement(78495..79562)
                     /locus_tag="ACOIAF_000243"
     CDS             complement(78495..79562)
                     /locus_tag="ACOIAF_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033225 - ATPase-coupled
                     2-aminoethylphosphonate transporter activity [Evidence
                     IEA];
                     GO_function: GO:0033226 - 2-aminoethylphosphonate binding
                     [Evidence IEA];
                     GO_process: GO:0033223 - 2-aminoethylphosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HGZ2667279.1"
                     /translation="MKFRKLVSLGILSTLLLSVITGCNNASSGSNLKSNNKIKGELTV
                     YTAIEEDSIDTYLTTFKEKYPDIKLNVLRLSVGDITARLLAEKDNPKADVLWGVAATS
                     LIILDDKEMLEPYAPKGFDKVNPVFKDDKKVPSWVGIDAWMTGITVNTKELSDKNIPV
                     PSSYEDLIKPEYKGLISMPNPASSGTGYLTVSELIQIMGEEKAWQYMDKLHKNIGVYT
                     QSGSAPAILAASGEYPIGISFGNRGIKLKGEGYPVEVVFPKEGSGWEIESNALVKKDN
                     IKEASKVFLDWAISKDAMNEYSKNFAVTTIDTGNPVPEGFPKNPLEQMIDNDLKSAAR
                     NREAILNKWIEKYDGKTEKES"
     gene            complement(79574..81262)
                     /locus_tag="ACOIAF_000244"
     CDS             complement(79574..81262)
                     /locus_tag="ACOIAF_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033225 - ATPase-coupled
                     2-aminoethylphosphonate transporter activity [Evidence
                     IEA];
                     GO_process: GO:0033223 - 2-aminoethylphosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HGZ2667280.1"
                     /translation="MITIKNKRRISDSKIQKCFLVFLVIALVVFILFPLISLLKNAFM
                     DTSGSFVGISNFSKYIENPTLIASFKNSIFVSTISSLISLCLAFIYSYAITRTNIRFK
                     NFFKTMGIFPLFAPAMLYGISLIYLFGNKGIFTIIGIEVPLYGPLGIIICQILFTFPQ
                     AFLILSVALSMADYRLYEAADSLGASEFKKFFKITIPGIKYSLVSSFFVSFILAFTDF
                     GAAKIVGGNYNVLATDIYKQVVGQFNIPMGATVSMVMLIPVLIAFTVDKIASKKQGMT
                     ITAKSMPYKIKENKFRDRVFLIACSFITLLIFILIFVSVIGSVIKLWPYDLSFTMEHY
                     TFKGISGNGIKTYLSSLKISILTAIFGTIFVFFSAYMIEKVDKFPRLRQISYFLSMIP
                     MALPGLALGISYITFFNSASNPLNFLYGSTTIMVIANIVHFYSVCFITSNSSLKMLDR
                     EYELVAKSINIPFYKVFFYVTLPMSITSILEVAVYYFVNSMVTVSALIFLYIPQTQTA
                     SISILKLEEIGYIGPSAAMAVLVFLTNIIVKLLYELTTKKLKTKTQKWQQKEVA"
     gene            complement(81280..82275)
                     /locus_tag="ACOIAF_000245"
     CDS             complement(81280..82275)
                     /locus_tag="ACOIAF_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2667281.1"
                     /translation="MSFLQVKNVGKSYGQVKVLKDISIDIEKGEFICLLGPSGCGKST
                     LLRIIAGLEDKHDGKIIINDKDMTNSPPESRNFGIVFQSYALFPNMNVYKNIAFGLEN
                     KNISKSDIDKKVRDILEVVELSGYDKKYPSQLSGGQQQRVALARAIALEPDFLLLDEP
                     LSALDAKVRLKLREQIKSLHSKLGITTIMVTHDQEEALCLADKMVVMNNGEVIQIGTP
                     KEVYKSPKTPFVADFIGTINFIDDDVNMIAIRPEDIKVEFNRDFKEIDVKVGEILDIE
                     FRGFNYRITVEYCSKQIKLDVVSKVVEQMKLCVGSKINFKIPKERTIQYKSEGCA"
     gene            complement(82712..83779)
                     /locus_tag="ACOIAF_000246"
     CDS             complement(82712..83779)
                     /locus_tag="ACOIAF_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033225 - ATPase-coupled
                     2-aminoethylphosphonate transporter activity [Evidence
                     IEA];
                     GO_function: GO:0033226 - 2-aminoethylphosphonate binding
                     [Evidence IEA];
                     GO_process: GO:0033223 - 2-aminoethylphosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HGZ2667282.1"
                     /translation="MKFKKLVSLGVLSTLLLSVITGCSTDSSGSDSKSDSKTKGDLTV
                     YTAIEEDSIEPYLATFKEKYPDIKLNIVRASTGDITARLLAEKDNPQADVVWGVAATS
                     LLVADDQGMLEPYAPKGSEEIESAFKDDKEVPSWVGIDAWMTGITVNTKELSDKNIPV
                     PSSYEDLIKPEYKGLISMPNPSSSGTGYLTVSALIQIMGEEKAWQYMDKLHENIGVYT
                     QSGSAPATSAASGEYPIGISFGYRGIKLKEEGYPVEVVFPKEGSGWDIEANALVKKDN
                     IKEASKVFLDWAISKDAMNEYSKNYAVTTISTGNPIPEGFPKKPLEQMIDNDLKSAAK
                     NREDILNKWISKYDGKTEKES"
     gene            complement(83791..85479)
                     /locus_tag="ACOIAF_000247"
     CDS             complement(83791..85479)
                     /locus_tag="ACOIAF_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033225 - ATPase-coupled
                     2-aminoethylphosphonate transporter activity [Evidence
                     IEA];
                     GO_process: GO:0033223 - 2-aminoethylphosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HGZ2667283.1"
                     /translation="MITSKNKRRISDSTIQKYFLIFSVIVLIVFILFPLVSLLKNAFM
                     DTNGNFIGISNFSKYVENPSLIASFKNSIFVSTVSSLISLCLAFIYSYAITRTNIKFK
                     DFFKTMGMFPLFAPTMLYGISLIYLFGNKGIFTTMGLEVPLYGPLGIIISQILFTFPQ
                     AFLILSVALSMADYRLYEAADSLGASEFKKFCKITIPGIKYSLLSSFFVSFILAFTDF
                     GAAKVVGGNYNVLATDIYKQVVGQFNIPMGATVSMVMLIPVLIAFTLDKMASKKQGMT
                     ITAKSMPYKIKENKFRDRVFLIVCSFITLLIFILIFVSVIGSVIKLWPYNLSFTMEHY
                     TFKGILGSGIKTYLSSLKISTLTAIFGTIFVFFSAYMIEKVDKFPKLRQTAYLLSMIP
                     MALPGLVLGISYITFFNSASNPLNFLYGSTAILVIVNVVHFYSVCFITSNSSLKMLDK
                     EYELVAKSINIPFYKVFFNITVPMSITSILEIVVYYFVNSMVTVSALIFLYTPQTQTA
                     SISILKLDEIGYIGPSAAMAVLVLLTNIIVRLLYEFVTKKLKNNTQKWQQKDVA"
     gene            complement(85497..86492)
                     /locus_tag="ACOIAF_000248"
     CDS             complement(85497..86492)
                     /locus_tag="ACOIAF_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2667284.1"
                     /translation="MSFLQVKNVGKSYGQVKVLKDISIDIEKGEFICLLGPSGCGKST
                     LLRIIAGLEDKHGGKIIINDKDMTNSPPESRNFGIVFQSYALFPNMNVYKNIAFGLEN
                     KNISKSNIDKKVKEVLEVVELSGYEKKYPSQLSGGQQQRVALARAIALEPDFLLLDEP
                     LSALDAKVRLKLREQIRSLHRKLGITTIMVTHDQEEALCLADKMVVMNRGEIIQVGTP
                     KEVYKNPETPFVADFIGTINFIDDGINKIAIRPEDIKVESNRDSKDKDIKVGEILDIE
                     FRGFNYRITVEYRSKQMKLDVVSKVAEQMKLCIGSKINFKIPKEGIVQYKSEGCA"
     gene            complement(86493..87329)
                     /locus_tag="ACOIAF_000249"
     CDS             complement(86493..87329)
                     /locus_tag="ACOIAF_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HGZ2667285.1"
                     /translation="MLTELHCHTSISDNNFTTEEIIKKAAEKNINILAITNHDTLSGL
                     DEALVLGEHYGITIIPGIEISAYDYKNRKRVHILGYNIDLNSQEIKNLCNPMVCDRHK
                     ASVKMVNKIIDLGYKISLEDVKKYSSKTGIFKQHIMKALIDKGYTDNIYSNLYKELFH
                     RGNGKVYVSLKYVDYRDAIKAIKNSGGICVLAHPGQMDNFSAIEEMVKVGLDGIEVYH
                     PSHNKELEKESLSYAKKYNLVVTGGSDYHGFYSQHNYELGSKSLNKEDLFKFRLALGG
                     VL"
     gene            complement(87333..88502)
                     /locus_tag="ACOIAF_000250"
     CDS             complement(87333..88502)
                     /locus_tag="ACOIAF_000250"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HGZ2667286.1"
                     /translation="MIKLIHNANIVLEDMILENGHLIIEGDTIKKVSNDKIDYAFDFN
                     EIDEIIDAKNLYVIPGIIDIHSDAIEKEIEPRPSTLLPFNMAFYELDKKLPANGITTV
                     YHSISLGDGVGVRSIDNSLKMIKNIDSYKNIDSKSINHKVHLRYEVLYYEGLEKVLEL
                     LDENKIDYLSIMDHSPGQGQYTNPTFYKEYATKVWGVTENYVDTWLDDLVNLHDNLDW
                     NKIANIIGIAKTKNINVASHDDDTLEKMDFIKNIGINVSEFPITLEVAKHSKKLGVLT
                     CLGAPNIVRGKSHNNNLKAMDAILEDCCDIVCSDYLPSAMIKSMCIVAERINNLNKAV
                     SLFTSNPAKAVGIYDERGSIKENKKADLVLVDIDSEYPKVVNTIVNGKTVYKREV"
     gene            complement(88503..89216)
                     /locus_tag="ACOIAF_000251"
     CDS             complement(88503..89216)
                     /locus_tag="ACOIAF_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HGZ2667287.1"
                     /translation="MKETILEVNNFSKDFELHALNKTITACSNISFEVSKGEFLGIIG
                     KSGAGKSTILKSIYKTYIPTTGEIIFNSEIYGNVDLAKIGDREMINLRKKEIGYVSQF
                     LKTLPRITAIELVVHSLIESGFEKDCSYDMAKDILNQFEIKENLWDAYPNNFSGGEKL
                     RLNLAQAMVKKPRLLLLDEPTASLDNQSKIYVKEKMLELKSQGTTMIGIFHDIEFMET
                     VIDKTFTMTKGTISESGVA"
     gene            complement(89257..90123)
                     /locus_tag="ACOIAF_000252"
     CDS             complement(89257..90123)
                     /locus_tag="ACOIAF_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGZ2667288.1"
                     /translation="MSKINDSKRLENIDKQVLIGRNINLIYGDGCEKCFESTGYESNN
                     ICKYCGSIVACNNVNLELYEGEVLGIVGESGSGKSTLLKILFFQEHANSGEVYISHYN
                     KDTNILELSDQKKRYIKNHFMGIVYQNPHLGLNLDFSSGGNIAEKLLMANLYNVEKIR
                     DRGKELLIKTNIPVERIDHKPKYFSGGMQQRVQIAKALSNNPPILLLDEVTTGLDVSV
                     QAEVLDLIREIQRELKISMIVVSHDFDVIKMLADRTIVMKNGSVIESGLTDQIMEDPQ
                     HPYTQQLINSLL"
     gene            complement(90116..90985)
                     /locus_tag="ACOIAF_000253"
     CDS             complement(90116..90985)
                     /locus_tag="ACOIAF_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HGZ2667289.1"
                     /translation="MIYNYGFLDENTKKEIRRKILKAISIPGYQVPFGSRELPIAKGW
                     GTGGLQLTLSLIGKNDVVKVIDQGSDDSTNACNIRSFIASVSNVETTKDTLEATLIQT
                     RHRIPEEKLKSNQILIFQVPIPETLRIVEPSEVETRRMHSEEDYSRMWVYLYEDIVRF
                     NDISIAVEYPCKVNDRYLMNPSPIPRFDIKKLNMSDNLFLFGAGREKRIYAIPPYTKV
                     EPLEFEDYKFEEEKFEGKYCSLCNSTNTFLDEVYDSDTNEKYYSCSDTSYCEKVRLKN
                     NSIDVTIGGTWNE"
     gene            complement(91005..92090)
                     /locus_tag="ACOIAF_000254"
     CDS             complement(91005..92090)
                     /locus_tag="ACOIAF_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-phosphorus lyase complex subunit PhnI"
                     /protein_id="HGZ2667290.1"
                     /translation="MAYVAVKGGEEAILQAKNILEFYRVKGDSLPIDVKQIEEQMSYA
                     VDKVMSEGSLYNKELSALAIKQAQGDILEASFYVRALRSTMPRITYSEPINTEEMRIK
                     RRISATFKDIPGGQYLGATRDYENRILNVELLEEDEKKNRHFRDSFINELETVDEIPE
                     FTKISNIFRKENLLQEEVCENKLEEVIDDITRNSLRFPVSRSAKLQALTRGESGVVSG
                     FAYAIVRGFGDEHPYISELRTGNVFIKVKHPIYEDEEIIIGDMLVTECEIVSKASQNK
                     TENSKTNSEDKNKMSLGYGLCMGNNENKAISMAILDKSLEPNSEKLAQDEEFVLLHID
                     GIDSLGFISHFKLPHYVDFKADVERIK"
     gene            complement(92093..92668)
                     /gene="phnH"
                     /locus_tag="ACOIAF_000255"
     CDS             complement(92093..92668)
                     /gene="phnH"
                     /locus_tag="ACOIAF_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HGZ2667291.1"
                     /translation="MVNYTQEIYRKMLDSASFPGKLNNIDNINIENNTKLLNGAIGIA
                     ITLLDQEVTFYIENYTQKDIKNIRALTMSNQIDYRKSDYIFLDINSEVDILQIKIGSF
                     EYPDESATIVHQVNDLSTYSQTKYIKLDLSGPGIKSKNSIYINGIREEFIERLSSINK
                     DYPIGIDLILVDKKGEIAFIPRSSKLSWEVL"
     gene            complement(92683..93114)
                     /gene="phnG"
                     /locus_tag="ACOIAF_000256"
     CDS             complement(92683..93114)
                     /gene="phnG"
                     /locus_tag="ACOIAF_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HGZ2667292.1"
                     /translation="MYTVDSTKILINMNLEKLEKIYSMIKDTSSISIIKEPNLATVMV
                     RANESVKNTTFNLGEILVTECSVKVDESLGYGIVSENNNKKAIYLAVIDAVLHSNNSK
                     FDELKNYINKSVYEENLRYEQEIIDEFSLINKTKVQFNAMD"
     gene            complement(93452..94222)
                     /locus_tag="ACOIAF_000257"
     CDS             complement(93452..94222)
                     /locus_tag="ACOIAF_000257"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HGZ2667293.1"
                     /translation="MLFDSHAHLNDESFDEDRDELIGSLKDKGVDLVVNPGADIETSI
                     TAIELAKKYDFIYSAVGVHPHDVSKLDDTAIETLRKLATENEKVVAIGEIGLDYYYDY
                     SPREEQKEWFKKQIELANELKLPIIIHDRDAHGDTFEIIKNTKNPEIGCVLHCYSGNV
                     ELAREYVKMGCYISIPGTVTFKNNKKTREVVREIPLERLFIETDSPYMSPEPHRGKRN
                     NPSQVSFVADKIAQEKGISYEEVCRVTKENAKKFFNIK"
     gene            complement(94252..96189)
                     /gene="metG"
                     /locus_tag="ACOIAF_000258"
     CDS             complement(94252..96189)
                     /gene="metG"
                     /locus_tag="ACOIAF_000258"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HGZ2667294.1"
                     /translation="MSKPSFYVTTPIYYPSGGLHIGHTYSTVAADTIARFKRFCGYDV
                     KFLTGTDEHGEKIQKKAIEQGMSEIEYLDGMIKDIKALWNTMDISYDDFIRTTEKRHT
                     DIIQKIFTKLYEQGDIYKGEYEGRYCTPCESFWTESQLLEGNKCPDCGRETYLVKEES
                     YFFRLSKYEDRLKELFKDDSFCFPAARKNEMVANFLDKGLEDLSVTRTTFDWGIKVPF
                     DEKHVIYVWVDALCNYITALGYMTDNDEEFKKYWPANVQIVGKEIVRFHTIIWPALLM
                     ALGLEVPKQVFGHGWILFADDKMSKSKGNVVYPEPIIERYGIDTLKYFLLREFAFGQD
                     GSYTHRNFVTRINYDLANDLGNLISRTVAMVEKYNNGIIPTAKVSTNFDADLKEQAVS
                     TRENFEAEMDKMQFHEALESVWKLVRRTNKYIDETMPWALAKDETKKDELDTVLYNLC
                     ESIRIIATLINPIMNETANKIYEHIGIKGQDDITTWESTKTFGLIGENVKVFKGEPLF
                     PRLDVEKEIEELTKMFSGKPPVEEKPLEHKEEITIDDLDKIELRVGKIISCEKHPKAN
                     KLLVSQVKIGPETRQIVSGIAEYYKPEDLVGKEVTVVCNLKPVKLRGVESQGMILAAG
                     DDGDPYVLPFTQGAKDGCEVR"
     misc_binding    complement(96273..96519)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(96585..97106)
                     /locus_tag="ACOIAF_000259"
     CDS             complement(96585..97106)
                     /locus_tag="ACOIAF_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein"
                     /protein_id="HGZ2667295.1"
                     /translation="MEIFSNLLIPIIILYIVVYGKYKKIDVYDSFVKGAIDGLKAAWD
                     ILPYIIGIFLAIGIFKTGKGLDMLEWLFTPIANIMSIPKELIGLISVKPLSGSGALGM
                     YSELANRVGIGSLVEKMGATIVGSSETIFYTMAIYYGSLKIKNTRHTLSCAMISHVAG
                     VIAAVFICYVIFV"
     gene            complement(97122..97706)
                     /locus_tag="ACOIAF_000260"
     CDS             complement(97122..97706)
                     /locus_tag="ACOIAF_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein A"
                     /protein_id="HGZ2667296.1"
                     /translation="MIIIGKIWFYMVSIGIIGSLISKNMGELNKVILSETSKGIEFAI
                     SLAGIMALWMGIMNIAKESGLIEKIGQKLNPIMRRLFPSIPKGHKAMSYIVMNIALNM
                     LGAGNGATAFGLKAMSELQTLNNKKDTATNDMIMFMVINISSIQIIPFTIIKLRMDMG
                     AQNPSEIIFTTLFATIISTAVAIITCKFFQRRYR"
     gene            97838..99268
                     /locus_tag="ACOIAF_000261"
     CDS             97838..99268
                     /locus_tag="ACOIAF_000261"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HGZ2667297.1"
                     /translation="MRNLTLLTDLYQLTMLNGYFEKNIHEDIVVFDMFFRKNACDGGY
                     TIVCGIDQVVEYIDNLHFSDEDLEYLKNLNLFSDKFLKFLKEFKFTGDIYAVEEGTIM
                     FPNEPLITVKAPLYQAQLIETALLTIVNFQSLIATKASRVCFAAQGDPVFEFGLRRAQ
                     GPDAGIYGARAAVVGGCAGTANVLAGKMFDIPIIGTQAHSWVQKFDNELEAFQAYADV
                     YPDKCLLLVDTYDVLNSGVPNAIKVFKNISEKGYKPMGIRLDSGDLAYLSKEAKKQLD
                     NAGFSDISITASNDLDEYTITSLKAEGATINSWGVGTKLITSFDSPSLGGVYKLAASC
                     EKGVLEPKIKISENPEKINNPGYKKVIRIYNEDNKAEADLIMLHDEVIDESKPLEIFH
                     PTYTWKTKVFTNYKVKELLKPLYIKGRCKYNKKAVLEIKNHVQYELSTIWEQYKRLSK
                     PHIYKVDLSRNLWYLKTQMIDSKKVL"
     gene            99305..99733
                     /locus_tag="ACOIAF_000262"
     CDS             99305..99733
                     /locus_tag="ACOIAF_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007287587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HGZ2667298.1"
                     /translation="MDVTDYRIIEILQDDGRISMKDLGKIVGLTSPAVSERVKRLEES
                     GVIEGYKAIVNPDSLGRVIKAFIHISLPSNGYTEFIESAAKDPRIVECHHITGDDCLL
                     LKVIVKDMYELENVIDTIKKIGSTKTSVILSTPIQAKSIL"
     gene            complement(99921..100625)
                     /locus_tag="ACOIAF_000263"
     CDS             complement(99921..100625)
                     /locus_tag="ACOIAF_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HGZ2667299.1"
                     /translation="MEIGKIIIESLKREDTHKIFIELDNKHELEKIIPKISCMKSVGE
                     CKYHVVNCFEHSINALKELEIVLNDKDFFPIHLRKHVSNYLKTYIEEGINKLNVLKLG
                     TFLHDIGKPDSMTLDETGRVHFTNHEKIGAQIIDNMGIKLELSTETYRLISKYVRYHM
                     ILLSLYKKNDLSRKELINVFNLVDEDTIGVIMLGYADIVSTVKLLGKSGEVSVLKTYM
                     EYILTNYLYKSNYTHV"
     gene            complement(100648..101481)
                     /gene="rsmI"
                     /locus_tag="ACOIAF_000264"
     CDS             complement(100648..101481)
                     /gene="rsmI"
                     /locus_tag="ACOIAF_000264"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HGZ2667300.1"
                     /translation="MSGKLYICPTPIGNLEDITYRTLRTLKEVDLIAAEDTRHSIKLL
                     NHFEISKPLTSYHEHNKDSKGDYLINKLIDGENIALISDAGMPGISDPGEEIIKQAIQ
                     NNIEIEVLPGATAFVTALVGSGMDTHRFIFEGFLDRDKKVRKAQLEELKEESRTIIFY
                     ESPHRLKDTLKDMLKILGNRNISINRELTKKYQEIIRENIETTIKIFEEKEVRGEFVL
                     IVEGFHGEKSKKENYDGLTDREYVLKLIESGMNKKDAIKIVCKDRKLKKDVVYKQVLD
                     L"
     gene            complement(101482..102228)
                     /locus_tag="ACOIAF_000265"
     CDS             complement(101482..102228)
                     /locus_tag="ACOIAF_000265"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HGZ2667301.1"
                     /translation="MQLKDSERIDDLQLKGLKIIQDTNGFCFGIDAVLLANFTKIKKD
                     AKVVDLGTGTGIIPILIAGKSEAKKIIGVEIQEDVYEMATRSIKLNGLEERVEIINED
                     IKYIDKVLDINGYHVVTSNPPYMHIDGIKNPNDKKAISRHEVRCNLEDVIRAASRLVM
                     PRGKFFMIHRPTRLVDIITLGRKYKLEPKVIQFVHPRPQKAPNLVLVQFVKDGRPELK
                     ILDPLYVYGEDGNYTKELKAIYNNKDIGEL"
     gene            complement(102306..103199)
                     /locus_tag="ACOIAF_000266"
     CDS             complement(102306..103199)
                     /locus_tag="ACOIAF_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HGZ2667302.1"
                     /translation="MIKIVGIRFKSAGKIYYFDPVDFNIEQDMDVVVETARGLEYGKV
                     VVGPKDMDESELVSPLKPIIRIATEEDKKVYLENKEKAKETFELCQQKIKEHELTMFL
                     IDCEYTFDRNKLIFYFTAEGRIDFRELVKDLAAIFKTRIELRQIGVRDEAKSIGGLGP
                     CGRKLCCSSWLGDFQPVSIKMAKDQSLSLNPTKISGICGRLFCCLKYEHDVYSEAIEK
                     MPVVGALVQSDDGKGKVVEVNPLLEQIKVEFQDKTVKIYTREDIKILREPKKCDGCGG
                     KCKDNDGLDEATLRELKKLED"
     gene            complement(103196..104131)
                     /locus_tag="ACOIAF_000267"
     CDS             complement(103196..104131)
                     /locus_tag="ACOIAF_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGZ2667303.1"
                     /translation="MYFENIIGQDFAKKYLTNSIKKNKLNNAYMFEGMDGIGKKKFAD
                     ELSKLLLDYENLENSPDYVLIKPDGNSIKIAQIRNLQSDIVIRPHKDYKIYIINNAEK
                     MTVEAQNALLKTLEEPPNYAIIILVTNNKESLLETIKSRCDIIKFSPIPIEDLKRYLI
                     NTGIEEERAQLLAIFSRGSIENALNLSQSSEFSMMREDIQQYIQIMLDKNIVEILNIP
                     NNMEKYRGKIIALLDMMINYFRDIILLKENVNKNMLINVDKLVFIQNMSGKISYSQLS
                     KIIDIIEDAKSKIKSNCNFNISIQVMSLNIYEVIK"
     gene            complement(104134..104823)
                     /locus_tag="ACOIAF_000268"
     CDS             complement(104134..104823)
                     /locus_tag="ACOIAF_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HGZ2667304.1"
                     /translation="MKGKLIIIESGSDASGKATQTEILYERLKKEGYMIKKVEYPNYE
                     SPSSALVKMYLKGDFGKNASDVDPYIASTFFTADRYASFKTDWEEFYNEGGIIIADRY
                     TTSNMVHQASKMDICDREEYLNWLVDYEFNLFKIPQPDCVIFLDVPIEFSKKLMENRK
                     NKITGEEKKDIHESDIEYLEKSYNNALYIADKYDWKKINCVLDNKLRSIESIHNEIYK
                     IVLDSIKPMER"
     gene            complement(104954..106363)
                     /locus_tag="ACOIAF_000269"
     CDS             complement(104954..106363)
                     /locus_tag="ACOIAF_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HGZ2667305.1"
                     /translation="MFIINELNKIVNEEVISFHMPGHKKGQVYKKLGYKDILKNLYKM
                     DTTEILGTDNLHFPEGIIKNSQKKASRVFKSDYTCYLVNGSSCGIQSAIMSVCSPKSK
                     IITNRDCHQSVINGCILGDIDIEYVPCEISEETNVLKGISISNVIDIIDKNLDAKAVL
                     LTYPTYYGMTYDLEYICNYAHSKKMVVIVDEAHGAHLGLSERLPKTALEQGADIVVQS
                     THKTLPAFTQSSMIHIQGDRVDIDKILNMLRITESSSPSYLLLSSLELAVDIYENKGK
                     ELMEELLNNIITFKNKVDSNINIYDTNDKTKIFISSKNMGLTGYELENILRNKYNIQV
                     ELSNYYGVLLICTIGNTAEDFSSLETALNNVVKEDFKFEKLENKKYPVVIPKKILTPR
                     EAFYKTKKSVKIYDSIGKVCGECIVPYPPGISVISPGEVISKEIIDYLMFCHSKGMIV
                     SGLKDVDLNFIQVIDLEDI"
     gene            complement(106518..106709)
                     /locus_tag="ACOIAF_000270"
     CDS             complement(106518..106709)
                     /locus_tag="ACOIAF_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HGZ2667306.1"
                     /translation="MKKVALILAIIGALNWGAIGILGTDLIGSIFGGTYEMVSRIIYF
                     IVGLAGLYLIPSLMSDDRE"
BASE COUNT        33278 a        19264 c        11678 g        42604 t
ORIGIN      
        1 taatataaat ttttcatata acaaatatcc taaacttatc taaaataaat ttaggatatt
       61 ttatttttat actctattta cagacccaca cactttaatc ttttctttca caaagtcctt
      121 tactaattga agccctgcta atccatactt tttagggtct ataacctcag aatcttcatc
      181 taaaactttt ttaacccctt tactatatgc aattcttaat tcagttgcat agttaacttt
      241 tgcaatacct ctttttatag cttcttttat aatatcatca tgtattcctg aacctccatg
      301 caatactaaa ggtattgaaa ctacattttt aatttctgat aacctatcta aatctatttt
      361 aggttcaccc ttatacaatc catgtgcagt acctatagca atagccaatg aagatattcc
      421 tgttctatcg acaaactctt ttgcctcttt tggatttgta taatcattac tattcccgcc
      481 atctaaatca tcttcttttc caccaacttt cccaagttct gcttccactg gtataagaga
      541 aggactacat gcttttacta ctgatttaga tatagatata ttattttcaa agctttcatg
      601 agaaccatct atcattattg atgtatatcc acttcttaaa gctttcattg caaggtcaaa
      661 acttgaacca tggtccagat gcattgctac aggtatattt actctttctg cagcaacctt
      721 tgcatttgct agataataat caagacctgc atattttaca gtagatggag tagtttgtaa
      781 tattactgga gaatttaact cctcagcagc ttctataaca gcaattacca tttccatatt
      841 ttctacatta aatgctccta ctgcataccc ttcatcttgt gcctttttaa gcattgatgt
      901 tgttgttact aatgccacat tgataccctc ccaaattatt tagttataat attttaatct
      961 tatcttttaa tattcatata ttgttacacc ttttactact cgatttactg ttcctgtagc
     1021 tgatggagta tctggcttat ttccaacctt taccgataca aacaatgcta tagtttgtgc
     1081 aaacaatatg tatggcattg caagatacac atctggtaat ttattatact tttctttaaa
     1141 ctcaaacttt gttcctgaga agttattttc attttctact gacactacac aagtgaattt
     1201 agcaatttta tcccttttta tctcctctaa aacatctaaa tcatattttc ttgtatataa
     1261 acagttagat acaaattcaa atacaagtgt attttcatca ataaatgatt ttggtccgtg
     1321 tctaaacccc ataggagaat catacactgt agatattttt cctgctgtaa gttctaaaag
     1381 ttttagttgt gcttcttgtg tcaaaccacc taatcctcct gaacctaaat atacaactct
     1441 atcaaaatct ttatttatta actcatgtat ttcatctttt ctatctataa catttcttcc
     1501 catttcaatt attgcattta tatatgactt ttctacatca tcttctaaac tatcgaatat
     1561 taacatagca gataacatca tacacgaaaa acttcctgtc atagcaaaac cttcatcatt
     1621 tgattttgat ggcattaata acaagtaatt attttcgtcg tttttagtca tcttagctag
     1681 ttctccttct ggattacaag ttatagctaa atgataaaaa ttatctacaa tcttattccc
     1741 taaatttaaa gcagccaaac tttcaggact atttccactt cttgcaaatg aaattaataa
     1801 tgttggtata tcttttttta agtaatcata tgggtttgaa actatatccg tagtaggtat
     1861 tgcttcaaat atgtattttc ttatgtcatt tttattctta aggtaaggaa gtattgaatt
     1921 tcctatgtaa gctgaagttc cagcacctgt aaatataact ctaaactgcc catgattatt
     1981 acttattgca tctatgaatc tacttaattt ttctttgtta gatttatata tttcataagt
     2041 ctctaaccat aattcaggtt gttgttttat ttcattagtt gttattattg cacctaaatc
     2101 ttttagtttt tgttcttcta atttaaacat tttcatgctc ctttcatact tgtaacgttt
     2161 acatttggta atgaggttat aaccacttta aactataaaa aaatatactc ttttataatt
     2221 ttttgcttag attttttagt actacttaaa ctcactataa aatatacact atagatttta
     2281 attaatttta tagtttatct ttaaaaattt aataaaatca tactctatat actttagtat
     2341 ttttatttta cgataaaaac ttgttcagac caaagtggta attaccacta atccaaatat
     2401 aaattaattt gaattttttg tcaatacata aaatatttat atcaatcaat tttatggata
     2461 tcctacaaaa ttactttatc aatatttctt atttcgctaa tttacttata taagctttaa
     2521 atttattttt tgatacaaag tttacaaatt tatgtgtctg aattataatc aattctgtaa
     2581 atataaaaac cttaggagaa agtattatga aaaatataaa aatgggaatg ataggtcttg
     2641 gctccatagc tcaaaaggca tatttaccta tattaactaa gtctgagaga tttgagtttg
     2701 taggagcttt tactccaaat aaagttaaaa gggaaaaagt ctgtagtgat tatagaatca
     2761 tgccattcga ttctattgaa tccctagcta aaaaatgtga ttgtattttt ctacatagct
     2821 ctacagaaac acactacgaa attataaaaa tattattaaa tttaggtgtt catgtatatg
     2881 tagacaaacc tcttgcttca acagtaagtc aaggagaaga acttatcgaa ttaagtacga
     2941 aaaaaaactt aaatttaatg gttggattca accgtagatt ctgtcctatg tacaaagaaa
     3001 ttaaaaataa tgctacagaa attgtatcta tcaatatatg taaacatgga ttaaattcct
     3061 taagaaatgt taaattcgac agtactctta tagatgacta tatacatgtt attgacactg
     3121 ctttatggtt agcaaatgaa gatgttgaaa ttagtggaga ggatttattt ttaacagaca
     3181 ataagaatct tatatttgta agtcataaac ttaaaggtaa aaatttctct ataaacacct
     3241 ctatgcatag agattctgga acaaagttag aacaagttga aattctttca aagggtaata
     3301 ttcaaagagt taagaatctt aatgtactgg aaatagaaga aggtggaaac ctaactttaa
     3361 agcaaagtgg tgcatgggta aacatattaa aacaaaaggg ttttgaggat atatcaaatc
     3421 attttattga ttgtatagaa aataatataa aaccagctat taatggagaa gagtgtatca
     3481 aggctcaaag attactagag aaaattataa attcagtaaa atagatattt attataaaaa
     3541 taggttaaaa atgaattttt ctaacctatt tttatataag actatatctt ttatattaaa
     3601 attaaaatct attacaacaa cattaaattt attatattcc cattaaatgt atttaactta
     3661 acttttttac ttcaactaaa tcttgatatt tatcaatttc taccaaatct acaaatccag
     3721 tttccatatt taaagcatta cttataccac aagcatttgc aaatttaagt aaattttcta
     3781 ttttataacc ccttaaaata cctacactaa atccagccac tgtgctatct ccactaccta
     3841 cagtatttat acactttaca actggtactt ttacatcata tacattatct tcgtttaaat
     3901 acaccatacc atcttttcct aaagatatac atacattctc tgcacccatt tcaataagtt
     3961 tttttcctga caagataatt tcttctcttg attctatatt cattcctaat aaatgtctta
     4021 tttcatctat atttggcttt attaaatatg gttttgattt taaagctatt tttaatgctt
     4081 ctcctgatgt atctaatata aactttatat tatttgctct acatatattt cctatctttt
     4141 cataatagtc tatcggcata ttctcacaat aactaccaga agctaccagt attttagtat
     4201 tttttagtac ttcctctata tcattttcaa atctctctaa atcgcttttt tctactattg
     4261 gtcctttttc taaaaattct gtactaactt tattgtcctc aattatgttt aagcaatttc
     4321 tagtttcctg ctctattttt gtaaatttat tttgtatatc tagttttcta agttcatttt
     4381 ttataaattc tccattaaat ccgcctagaa atcccatagc taaaggattt tcttttaaaa
     4441 ttttacatac cttagctacg tttaatcctt tacctccagc agttgcattc ttacttacta
     4501 ccctttgcac ctcacctatg tttatattat tcactctata cattctatct atagatgggt
     4561 tgaatgttat tactgtaatc atactagatt ctcctcatta ttaattttta cttttctaaa
     4621 caataaatta atctgtaaaa taaaaaatac ctcatataat atatccaaag tggttattac
     4681 caatttgaat ataaattatt ttacttgttt tgtcaatgta ccataatttt ataataaatt
     4741 aatagcctta cttattgaat tttgattaaa ctttattcat tgacataaat aattctatat
     4801 tctataatca aattgtggta ataaccactt agaaaggact atatttttat gataaaaaga
     4861 aatgataaaa gaccaatata tgaccagctt gttgagatat taagaactaa gattgaaaat
     4921 gagatggaac caaacgatag aatgctttcc gaaagaaaaa tttgtgatga atatggagta
     4981 agtagaacca cagttagact agctatggct gagttagagc atatgggata tatatacaaa
     5041 agacatggaa aaggaacttt tgttgctgca ctaagtcaaa attctcaaaa cttaatggaa
     5101 agttatagct ttacagatta tatgagaaca caaggaaaaa agccaggtac aaaggtatta
     5161 tcatttgagg tagtagaaag tacaaagtat ttctctgaaa aattaggtat aactcctggt
     5221 gaaaaaatga taaaaataat gcgtttaaga cttgctgatg accttcctat gatgcttgaa
     5281 agaacatatc ttcctatgaa agaatttata ggattgacaa aagaaaaaat cgaacaaaaa
     5341 cctttatatg aaatatttag agaagattat ggcgagatta ttaaagttgc tgatgaagaa
     5401 ttttcagctg gaataatctc tgataaggaa gctaagttat tggaagtacc aggagattca
     5461 ccttgtctga aattacttcg taccacatat aatgatgaca atatagttat tgaatttact
     5521 ttgagtgtcg ctagaagcga taaatttgta tatagagtaa gacatgtaag atagtaagtt
     5581 aaatagcttt caagaaaata gaatgatatt ataagcgtca atgccacaat gatagtataa
     5641 ttataaggag gttattttga aaagttttat ttatgcagat aaatttttta tgaaacatgg
     5701 aatcaaagaa aaaggttacc ttagtataat taatggcaaa ttcggtgaat ttcaacaaga
     5761 aagacctaat gttactgata atgttataga tttttcaggt aaatatattg ctcctggctt
     5821 agttgatact cacattcacg gtcttttagg tgcagatgtc atggacaata catttgaagc
     5881 aataaacaca atttcaaaag gcttattaaa atatggagta acatctttcc tacctacaac
     5941 tttaactgat tctgtagata cactaagtga ttctgtagaa aatataagca atagcttcaa
     6001 tcgagttgag ggtgcaaaaa tacaagggat ttatttagaa ggtccatttt ttacagaaaa
     6061 atataagggc gctcaaaatg aaaaatattt taaagaccct gacatcaata tattaaaatt
     6121 atggcaaaaa aaatcgaaaa atttaataag aaaaatagca attgctccag aacgtaaagg
     6181 ttcaatagaa ttaacaagat atgcaactaa aaataatata tcagtttctc ttggtcatag
     6241 ttcagctaca tttgagcaag ttaaacaagt cgttaattca ggtgctaaag tgtttgtaca
     6301 tacatataat gggatgagtc ctttaaatca tagagaacct ggaatggttg gagctgctat
     6361 ggtactaaaa gatacttatg ctgaattaat ttgtgatggt catcatgtaa gcccaatagc
     6421 tgttaaaata atgatggatg cgaagtctag taataacata gctttaatta cagattgtat
     6481 gagagctggt gctatggagg atggaaaata tactcttggt aagtttaatg taaatgtaaa
     6541 aaatagtgtt gcaagattaa atagtgggag tttagctgga agtgtattaa ctatggacaa
     6601 ggctgtgaga aatatagttg attggaacat atcaacttta gaagatgcaa taaaaatggc
     6661 tacatatgtc cctgctttaa gttgtaatat tgataatgtc tgtggctcta tatatcaagg
     6721 tatggatgca gactttatag taacagataa tgactttaat atacacgaaa cttatataaa
     6781 tggtatctgt aagtacaaag ttaataaata ggttcaatat aaggttatgg aggctgtcta
     6841 aaaagagtct ggaaatattt aaagttattt ctccaaaatc tgatttaaca cttttatttc
     6901 catatcgtta ataaactatt gcgatagagg ttattttaaa caaatatata ttataagtat
     6961 ttttgtgata atattttcga taatatgtac atttaaagta acttttctat tattatttgt
     7021 aagtttttat cttgagcaaa catataaatt tttaataaag catgagataa accctatatt
     7081 tcaattacta atgatgagat tttgattgat aacagatata ttttcaaatg taaaaatact
     7141 aaaaatcata tcaattttaa agatattgta ggtgtaaata aacaagataa tactatagaa
     7201 atttttacaa atgaaagcac ttattgtata tatctaaaag ttttaagcat tgagagcgaa
     7261 gaatatctaa tagatagact aaaatcattt tgttgatttt atatatttta tgttaaatat
     7321 aaagcagagt agttaaaact accctgcttt tgtaattaaa atttaaaaat aataactcat
     7381 aatacattga atttgagctt aacagtaact attgtaataa tagtatcaat cttttgtgtt
     7441 gaatataatt tatttacact atagttaagc atgatttcta atgttatttt ctctaattta
     7501 caattatgat taaaattcat taatatatat atttataagt tgtattgttt atattgttta
     7561 ttactttttt aaatccacta gctgtaataa taaatgtctt ttctggtatc cctggtgaaa
     7621 agattatttt atcatggtta aatatatctt gatctattac aagagagata tttttctcat
     7681 atccaaaagg agatacacat ccaggcaaac attgtgtttt ttgtgttaat tcttcttttg
     7741 aacatataga tacttttcca cctaaaacat tttttatagt ttttggatct aattttttcc
     7801 cttctgtggt tacaaatata tagtaatttc cagatctatc ttttataaat aaacttttac
     7861 tttctactcc agtatattgt aatttttctc ttatttcttt agctttttca aatgataaaa
     7921 taggttcatg gtcatattct tcaaacgtta tatgattttc ttttaaaata tttttgatat
     7981 tgttgtacat atattttcct ttctatattt aaattaattt atatataata ttcaattata
     8041 aagtgatttt ataatttctt caaactatca taaaaaacat tcacatgttt taggaataaa
     8101 ttatataaaa taggttataa gataatgaac atttatatta taacctattt ttatatcgat
     8161 aagtatgaaa ttgtttaacc aagtcaaaat gatatataaa tttatattta aatgtgttta
     8221 gagtttaatt tattttcaat gtttaaaaaa gttattctat gcatctaatt taattagtaa
     8281 actgaggttc tagttagata aattatatta taatgaatat atagtatgca gtttacgata
     8341 ttttaaaagt aaatgatata atatttggaa caactgggtt gcaatgtgtt tttaaaaaat
     8401 atcaaaataa ggctatgatt tttaaaacca tagccctgtc tgtaaaaatt taattatcac
     8461 ttaaaaaata aatttttatt taaagaatac acataaatat ttccagactc cctcatgaac
     8521 taaaaagttc attttgagac atctctttta taatgttctt tattttatag tataagttta
     8581 taaaaattaa atcctattta tcaatctgtt ctctaagcca cttagttgct acatctaact
     8641 gtttattttc atctttctct tcaatagtag gctttatacc tttttcattg atataatctc
     8701 cattaggtgt aaaatattga gcagtagtta acttgtatcc tgttccatcc ttcaatctta
     8761 caactgattg taccaaccct ttaccaaaag ttgtagtccc tacagaaata ccttctttat
     8821 tatcaacgat tgcagctgtt aatatttcag cagcagaagc actttctcca ttagttaaca
     8881 ctactattgg aagtcccaat ttttctttat cagatttcaa gtactcagta tttcccttat
     8941 tatcttttgt atatactata gttccttcac caattaactc atcagcaact tctttacata
     9001 tatctagcat tccaccagga ttatttctaa ggtctattac taatcccttg atattttttg
     9061 cttttaattc ttttaaagct tcatcaaagt cagtatatgt acttgatata aattgggtaa
     9121 tttctatata tcctaaatca ttatcaatta ctttactttc tatactcttc tcaattatct
     9181 ggtctgtttt tactttaaag ttcaattgtt tatcttctct taatatagtc agttcaacta
     9241 ttttcccttt tttacccttc atcatacgaa cagcttcatc tgaattttta tatgagacag
     9301 attttccatt gacttttact aatttatccc ctgattttac acctgctttc tctgctggag
     9361 aatctttcat gggaactact actaagccac catcttttga tgcccctata tacataccta
     9421 caccaacata agaaccttca ctatcattaa ttagattctc catttcatct ttagtataat
     9481 aaccagaata cttatcattt gttcctagaa atagaccttt tgatgctcca tttaccaaat
     9541 cagtatctga tactttttta taaaaatctt gttttatgat agattctagt cctaacaact
     9601 tatcatattt tttatagtct tcatagagtt ctttcgatat tacaacttta ttccccaatg
     9661 tcaactgaaa tgctgatgta accatagctg ttataattac taaaatcacc cctaaaaaaa
     9721 tagctttctt ctttgaaatc acaagataca tctcctttta cataaactat tattattata
     9781 tgactagctg ttatttttat tacatttacc caaatatatt gctttattac aataaattat
     9841 actaaaattt atattaaata caattgctgt acacattata acatattaag ggaggaatta
     9901 ttttatgaaa aaagaattta gaaaaaaagt tattgaatca agaaaaaata agagtgcaaa
     9961 cttcgtaatt agtaattcaa atttaataac tgaaaaatta cttcaaatgg aagatataag
    10021 aaatgcaact aatataatgc tttatttaaa ttttaacaat gaagttcaaa cagataattt
    10081 aatcaaaaaa cttttgtctc taaagaaaat agtatcaagt cccatcacaa taaaagaaaa
    10141 tcatacatta atccctactc aaataacaga tttaaaagag ggattgagaa ttggagaata
    10201 tggaataaga gaacctaacg aaaaatcacc tacagttgat attaaggcct tagatgttgt
    10261 aattgtacca gctgtagcat atgatataca ttgctataga ttaggatatg gtggaggatt
    10321 ttatgataga tttttagaaa acttaagaaa agatgctatt actatcggca tagcatttga
    10381 cttccagata ttcgattcta ttccaaaaga aactcatgat gcacaattag actacatagt
    10441 tactgaaact agaattatca ctccaaatag ttagaatatt aagtatttcc agaccccatt
    10501 taatgtagta ataaatacta ctgttaatat cttactttat aagcatttta agtcatggct
    10561 tttacaacaa taagaaccat atacaattta cttacattaa taaaaaccat gactttattg
    10621 tacaatatgc agttatttaa ttataaaata ccttcttatt tcgttgcatt taattctatt
    10681 ctatctggaa gttcactcat atttgtttca acaactatgt atggaaaaga atcttcctta
    10741 tctttatact ttatcttgac cctcaaaaca tcctttcctt cttcaaccac actttctatt
    10801 ttgtccattt ctataccata tttattattt cctctagtca ctataatgta tattttgtta
    10861 tcgattttag ccgctaatgc tctttcttca tttgtatact tatccatcac atctagtatc
    10921 ttttctggta ttgaatttct ttgtaatact gtataatcta tgggtttaga tttctctaca
    10981 aacatacttg gtatgaatat aaatgcccct ataacgaata caagagctat aactccataa
    11041 attatccctt taatattaaa ctttacattc aatcttatca ctcctcccat atattctaaa
    11101 tatatgattt ataaacttat cttagactct aaattattga ttagtatata tttatacata
    11161 attataaata tatgtaatgt aactaaaaaa catgtttttt ctatttcata ataaacaagc
    11221 taaaaataaa aagagctata taaaaacatt ttcattgttt tcatatagct cttgagttta
    11281 caatattaaa aaatatttat ttgtcactga ttccttcttg atatccagat gtttttgtaa
    11341 tctgtctata aagaacacaa accagtatta tgacgcccat atatccagta tatggatata
    11401 aaataccaac cagcttatca aatggtagga atccacctat aagacctaat atagctgcta
    11461 ttaaagttat taatttaaac ttaggatgat tatcatctac aaatctatta gttactgacc
    11521 ataataaagg tacagctgtt gtatatatac ctaatataag aattattgag aataaaactc
    11581 caattattgg tgaaatctta tctgctaaga ataatgatgg tattgctttt gaatataagt
    11641 ttcctatatc agaaagaata gctaagtgca tcattatagc tgcagccata agtgctactc
    11701 cacctaatat accaccacaa atagcttctt ttttgttagc agcacttgct cctaatccag
    11761 ttaagaatgc cataacaaca attgtgttat aagtagtata tattacacca gatagatagg
    11821 catttggaac tggtttagct atctttacag tgctaagaac ttgtgcagct tctcctatat
    11881 tacctatatt tttagctaaa cttataccac caactaaaag tgtaaatatt attattatag
    11941 gtcctattcc acctagtatc tttgataatc tatcaagacc taatgaaaca gttaataatg
    12001 ctactactgc catacctact ctacctacaa atggatttaa tccatagtat tcagttaaag
    12061 ttgctcctgc tcctgaaatc attactacga aaacaccaaa taagaataaa ggtccaaacc
    12121 attcaaagaa tattcctaaa tacttaccac aataaacacg ataaattttg ataggttctt
    12181 taagttgaag ctctttacct tttgacataa ctgaagcacc catccatgag aataatacca
    12241 ttgaaattat cccaccaata atacctgcaa aaccataaaa agagaagaat tgcattattt
    12301 cctgtcctgt tgcaaatcca gaaccgatta ctgttgctac atacgcacct gcgaaactga
    12361 tgactgtttt aacatttact ttttctgaca tatctcagtt acctccccat gtgaaatttt
    12421 ttcttctgct taaacaattc gataaactac ctacaatttc ctttaacata aaatacatga
    12481 taactgtttt atagcttttt agtataataa cttaaaactt ataatctaat tttataccct
    12541 agtcctcctt tcataatttt tttagattat tttatctaag tagtcattat atataaaatt
    12601 aatttctcta taaattatat attagtcata tttttctgaa tagtcaatat atatttgaat
    12661 attttctcta tttttgtaaa aatttgaata ttttattata tttttgatat attgatatca
    12721 tataaaaaaa agagagatat aaattaatat aacactttat acctctcttt taaatatatt
    12781 atattattaa caaacatgaa taatttatat ctcttacatt ctttttgcaa taatagcttc
    12841 tttaacagtc tttactatgt catcacaagt taattcatat ttttttaaca attcatttgg
    12901 ggtaccagat tctccaaatg tatctttaat tccaactttc tttacaatgg ttggacaaga
    12961 ctctcccact acctcactta cagcagaacc taatccacca attatgctat gttcttctac
    13021 agttacaata gcattcgttt cttttgctga ttctaatatt aaatctttgt ctataggttt
    13081 tatcgaggac atgtttataa ctctagcact tataccttct tcttgcaact tttcttgtgc
    13141 taatagagct tcatttacca taattccaca agctataatt gatacatcct ttccttctct
    13201 taagactgta ccttttccta tttcaaattt ataatcttca tcaaataaaa ccggtactga
    13261 acttctccct aatctaacat aaacaggtcc attatactta gctatttcaa aaataatttt
    13321 ttctgtttca actccatcag ctggaacaag aactgtcata tttggtatag ctctcattat
    13381 agcaatatct tctacacttt catgagatgc tccatcctct ccaacagtaa gacctgcatg
    13441 agtagcacat atttttacat ttagctttgg ataacaaact gaatttctta taatctcaaa
    13501 ggcccttccc gtagcaaaca tagcgaaagt acttgcaaaa ggtattttac cagcagttga
    13561 taatccacaa gcagcgccta ttaaattttg ttcagctata cccatattaa aaaatctatc
    13621 tggaaatgtc ttgtaaaaat catgtgtttt tgtagacttt gataaatctg catctaatac
    13681 tacaacattg tcatttattt gacctaattt tactaatgcc ttaccatatg cttctctcgt
    13741 cgccatttta ctcattgtca gcacctccta attcaagcaa ggctttttct agctcctcat
    13801 cacttggagc tgtcccatgc catcctgctt gattttccat aaaagatatt cctttgcctt
    13861 ttatagtttt tgcaactatc attgtaggtt ttttaactgt tgatttagct atgtcaagag
    13921 ccgcaaatat ttggtcaaaa tcatgtccat ctatctcaat tacattccaa ccaaaagctt
    13981 taaatttatc aactattgaa cctaaactca ttacgctttc tatatctcca tctatttgaa
    14041 gtccattatt atctaaaaaa gcaattaaat tatcaagttt ataatgtgca gcactcatag
    14101 cagcttccca aacaattcct tcttgaacct caccatcacc aagcaaagta tacacattcc
    14161 acggagcatt atccaacttt gatgccatag ccataccaca tgcaactgaa aaaccttgac
    14221 ctaaagaacc agtagatatt tccacacctg gcgtgccttt catgtctgga tgaccttgta
    14281 acatccttcc tatcttcctt aatccattca tttcttcctt atcaaaataa cctttttcag
    14341 ccaatgtagc atataatgct ggtgcagcat gtccttttga taatacaaat ctatctctat
    14401 cctccatttt aggatttgaa gaatccacat tcatttcatc aaagtataac gcagtaagta
    14461 tttcaactac tgataatgac cctcctggat gtccagattt ggctctatgt atcattgaaa
    14521 ctatatctct tcttatgata cacgcaatct catttaatcc tttatggtct ctcatgcaat
    14581 tacctccctt gagttcgtaa ttttatatca attccatatt attatatcac ttttatgcta
    14641 tttttttgcg ttatttttaa ttttattaac atagtacatt aaaattaaac cttaagagat
    14701 atttaaaaga aaaaagggat tgtctaataa aagacaaatc cctctttaaa aagtaaacaa
    14761 tcctaaactt atttgaagtc catcatctac ttttatcatc atattttcat ctagacaacc
    14821 aattttttcc ctcagtctct ttttatcgat agttctaatt tgctctaaaa gaattactga
    14881 gtctttatta agcccatatt cattggaact tatctctaca tgagtaggta atttggcttt
    14941 attaatctga gatgttatag ctgatactat aactgtagga ctatatttat tacctacatc
    15001 attttgtatt atcagaacag gtctaacgcc tccctgctct gaaccaacaa ctggacttaa
    15061 atcagcgtaa tataaatcac cacgctttat ttcacaatta gtactcacct tgggcaatct
    15121 tcccttcatt tttatcatca gaagatactt cttcaactgt catacctaat tcagctagtg
    15181 acagattaat ttgacccatt tctctataac ccgttttcat agattcttta atatgaattt
    15241 tctttttctg agttatatag aactgaaaag cagcttttgc aaaatcttct ctgttactgt
    15301 tctccatctg aactatacta tcaacttcag aaatcagaga gtcaggtaaa gtaacttcta
    15361 ttttttcttt actcttatcg tacacaaaat cgcctcccag attataatat catggtttta
    15421 ttttagcaaa taactgttta tttgtacaag ctcattattt tccatataaa cacggtcaac
    15481 tcttcttgat atcatacata acacttcata gtttatagtt cctaagtctt tagctatacg
    15541 ctcagctgta acttcgcctt ctccaaatag tataacctca tctccaactt ttatgtctat
    15601 atctttgtca attctaacca ttatttgatc catacatatt ctaccaacta catcaaacac
    15661 ttctccctta ataagaacct ttggattttt ttggattcta gtaaatccat ctgcgtatcc
    15721 tattggaact gtagctattg tttctttacc tgtagttgtg tattttagtc cataacttat
    15781 tcctacacct ggttcaactt gttttatatg tccgattttt gattttaatt tcatggctgg
    15841 tcttaattct aatctatctt taaatacatc atcagatgga taatgaccat ataatattat
    15901 tcctgctctt accatattta atcttaaatc agggcaatcc ataatagctg cactgtttga
    15961 tacatgtttt atagctattt ttacaccagc ctcatctaat ttatcagaca taaatttata
    16021 attattagct tgtttataag tgtactcttt acttacttca tcagctgtag caaaatgagt
    16081 aaacatacct tctaaatcta tatattctaa tttatttaat tctattattt cctgtactga
    16141 ctcctcatta ggttggaaac ctattctagt catccctgag tctattttaa catgaacaca
    16201 ggctttttct cctaaagatt ttgcaatttc atttatcttt tgtgctgttt ctaaagaata
    16261 aactgtcata gttattttat tttttataga atcttcaaaa gcttcgtctg gagtatatcc
    16321 caagttcaaa ataggaagtg ttatgccatt ttgtctaagt tcaattcctt cagcagttct
    16381 tgctactgct aagtaatcta ctttttcttt ttctagcaat tttgcaactt ctactgcacc
    16441 atgtccatat gcatcagctt ttattactcc acaaatctta atatcttctt ctaataaatt
    16501 tttaatatta tttaagttaa atcttaagtt atctagattt atctctgccc atgtaggcac
    16561 tgttattttt tgcatgtctt tagtccccct tagtttctgt attcaaaatc tttgtatttt
    16621 actgtaaatc ttggcactcc ctctttatct aacacttcca ttttttcagg attttttgtg
    16681 tcagcattta catataatac ttgcttgtta aaatatttat tatccccagg aataaaagtc
    16741 tctaaaacca aatgtccttt ggataacttt actttcatct cctcattttg aagataattt
    16801 tttataaagt ctcctacaaa aagatattga ttattttttc ctgtattagg taactccact
    16861 acatcactaa tctttggatt tttaactaat attttatcac cttgatattc tatacttttc
    16921 ccttttaaat gttttgggga aattacttct aatttatagt tatcggtttt tttataagta
    16981 tgaattaaaa cataattgtg tgggctttta tttccaacta cttctacttc agctttacaa
    17041 ctgtaataat tcatatcaga aatctgcttt tgaaaatctt tgtatacttc ttcttttgtg
    17101 gactgtcgtt tttggcatcc tattacaatt ataaccagtg ccaagaacat aatacatact
    17161 atggtccact tttttctcac atcacacctc ctacttcagt ttattcattg tataaagctt
    17221 ttattatatc atatctacaa attatacctt taagtttgtt gtcatcatct actacaggaa
    17281 ctctgtttat tgatttttta atcattacat tagctacatc atcaaactta tcatcttcat
    17341 gaacctttat tatatcttta gacataagtt ctccaacctt ataagcagct acttgtttta
    17401 tatctttttc aacttttttt aagtcatcta agaaaatcaa tccttgtaat agatttatat
    17461 agagaggtgc ttctatatat ttttcttttt taagaatatc tgtttcagaa attattccaa
    17521 caactctatt ctctgaatct acaactggca gacctcctat tttttctgca attagcatat
    17581 tagctacatc tgctatacta tcatcttgtt ttgctactat tacatctgtt gtcattattt
    17641 cttttgctgt tttatccatc ataatgtaat cactccttag tccttaatta tggttatcgc
    17701 atttgctata gcataatctt ttgaatgtga tattgaaact tttatttcta aaacattata
    17761 atctatgcac atttttgcta gattattata agtttttact attggttttc ccatttcatc
    17821 tctgagaact tctatatctt taaaattaaa cactctaatt cctgtaccta tagatttact
    17881 tatagcttcc ttggctgcga aattacctgc tatactttct gctttaaaat tcttagaatt
    17941 aaaatactct atttctctat ctgtaaatat cttctccaaa aacctattat ttttgtctac
    18001 agcctttctt attctatcaa tttcaattat atctacgcca atatcaaata tattcattgt
    18061 tcctccagaa aatttataat acttctttca ataattaata atgaatttat aaatttacta
    18121 tcgataatac acattattat ttagtttatg ttacttatct taatttaata atcattataa
    18181 taaaactgta acttttaaaa ttatttgtaa tgaatattat atacaaatct attatattat
    18241 ttattatact acacaactaa gcaaattttt taaattttat aaaaatttta tatgtatttc
    18301 ttatatgaag actttttcac ttacataaaa gctatctaaa tcttctttat cttattaatt
    18361 acaacataat ttggcagtaa atttaaacat tttgtgaatt tattatcact aaaaacttac
    18421 aattttatcg ttattttatt aacaccaaaa cacaaaaaag ccattttacc caaaagtttt
    18481 tttggtcaaa atgacttttt atactctaaa ttaatttatt aaatccactc agcagattct
    18541 gtcaatatga cagttttatc ttgagttaca ttaatgaatc cttctgctat tttcgcctct
    18601 ttaaaacttc cctctttttt tatctttaaa gttcctacag gtatacctga aacaaatggt
    18661 atatgacctt tcaacacccc tttgtctcct tttgtagttc taactactat catttcaaca
    18721 tctccatcat aaaaagtatt acttggagtt acaactttca atgaaaattc actggccatg
    18781 actacatact tcccttcgct tttctaacag cgtcttctat tgttcctaca aataagaatg
    18841 ctgactctgg taagtcatca tgtttccctt caagaatttc tttaaatcct cttacagtct
    18901 ctttaacagg aacatattga cctggatttc ctgtaaattg ctcagcaact gtgaatgatt
    18961 gagataagaa tctttgtatc tttctagcac gtgcaactat taacttatct tcatcagata
    19021 attcatccat accaagtata gcaattatat cttgaagttc tttatatctt tgaagtattg
    19081 attgtacacc tctagctact tcataatgtt cttttcctac tatactaggg tcaagaatcc
    19141 ttgatgtaga ttcaagaggg tcaactgctg gatatatacc cagtgatgat atttgtcttg
    19201 ataaaactgt ttttgcatct aagtgagaga atgttgtagc tggtgctggg tcagttaagt
    19261 catctgctgg tacatatact gcttgaacag atgttattga acctttctta gttgatgtta
    19321 ttctctcttg aagtgcaccc atttcagtag ctaatgttgg ctgataacca acagctgatg
    19381 gcatacgtcc tagaagtgct gaaacttctg aaccagcttg tgtgaaacgg aatatattat
    19441 caacgaaaag taaaacgtct tgtccttgct catctctaaa atgttctgcc attgtaagtc
    19501 cagttaaagc aactctcatt ctcgctccag gtggttcatt catttgacca aatactagag
    19561 ctgttttatt tataactcca gactcactca tttcgccata aaggtcgtta ccttctcttg
    19621 ttctttctcc tactcctgaa aacacagaaa taccaccatg ttgcttagca atattgttta
    19681 taagctcttg tattagaact gtctttccta ctcctgctcc tccaaataga cctatttttc
    19741 caccttttag atatggagct agtaagtcaa ctaccttaat accagtttct agtatctctg
    19801 ctgtagtttc tagttcatca tatgctggtg ctggtctatg tataggtaat ttaggtgcag
    19861 attttggagc tggtttccca tcaactggtt ttccaagaac attgaatatt ctacctaaag
    19921 tttcatctcc aacaggaaca cttattggcc ctcctgtatc tacaacttcc atacctctta
    19981 caagtccgtc tgttgcactc atagctatac atcttacagt atcatcacct atatgttgag
    20041 caacttctgc aactatttct ttatctccta actttattac taacgcattt aataagttag
    20101 gtaggctttg ttcactatca aacttaacat ctagtacagc tcctacaatc tgtactactt
    20161 tacctacatt tgccatttcc taacctccct attttagagc ttcaactcca ccaactatct
    20221 ctgttatttc ttgagttaca gctgactgtc ttgctctgtt ataacttaac tctaaattag
    20281 atagcatttc attcgcattg tctgtggctg attccattgc agttcttcta gcaccttgct
    20341 ctgatgcaaa agattccaaa attccaccat atactgtacc tgatataaac tgaggtacaa
    20401 tataacttaa aactgcttct gctgatggta tatattgtac ttttgatcta ttttgttttt
    20461 cttcttttac tgccattcct tgttcgtctg tatctttttc aaagactaat ggaagcaatt
    20521 tcaaaatttc aacttcttgt gataaagcag atttaaattt tgtataagca atataaactt
    20581 catctacttc acctttttta tacaggtcaa taatagtttg tgaaatttct gtcgcatctt
    20641 catatccaca agcttcaaca ctatttatat cttttgctat attgtagttt cttttagaaa
    20701 aaaattcatg tgctttcttt ccaacagtaa tcactgtttc ctttttacct ttcatatgag
    20761 agacagattc ctttaataca ttagaattgt atccacctgc aagtcctctg tctccagcta
    20821 ctactacata gcatttattt tttacttctc tttcttttaa aaattcattt ctaattccac
    20881 gtgttgtttg ggcaatatct tttactgtat tgtataaagt attaaaatat tctcttgaat
    20941 cttcagcctt ttcttttgct tttctaagct tagaagatgc aactaattcc attgctttag
    21001 ttatttgctt tgtactttca acagttccca tacgtgtttt gattgctttg attccagctc
    21061 ctgccaatta atttacctcc ttttttaaaa aggattatac ttcaacaaca aacttttctt
    21121 taaattcatt tattgcatct gttaaatcat gagtgaagtc ttctccacct aatatttttc
    21181 ttgatatttc cggataatta ttatctacaa atgcatacaa ttctgattca aatctagcta
    21241 tattatctat cggaatatcc tctaaatgat tatttataac agcatatatt atcataactt
    21301 gattttcaac ttttattggt tgatgctcac cttgttttaa tatctcaacg attctttcac
    21361 cttgagcaag tctcttttta gtatcttcat ctaagtcaga accaaactgt gagaatgctg
    21421 caagttctct atattgtgaa tatgcaagtt ttaatgttcc tgcaactttt ttcattgctt
    21481 taatttgcgc agaaccacca acccttgata ctgatatacc agggtcaact gctggtctta
    21541 cacctgaata gaataactct ggttgtaagt atatttgacc atctgttata gatataacat
    21601 ttgttggtat atatgcagaa acgtcaccag cttgagtttc tattataggc aaggcagtca
    21661 ttgaacctcc acccaattca tcagataact tagctgctct ttcaagtaat cttgaatgta
    21721 aatagaatac atctccagga tatgcctcac gtcctggtgg tcttctaagt aacaatgaca
    21781 tttctctata tgcaacagct tgtttactta aatcatcata cactataagt acatgtttcc
    21841 cattgtacat aaattcttca cccatagctg ctccagcata tggtgctata tattgaagtg
    21901 gtgctgattc agatgctgtt gctgaaacta ctattgtata atccaaagct ccaccttttt
    21961 ctaaagagct aactaattga gctatagtag aacgtttttg tccaattgct acatatatac
    22021 agataacatc ttttcctttt tgatttagta tagtgtcaat aactatagat gttttaccag
    22081 tttgtctgtc acctattatt aactctcttt gacctctacc aattggtatc attgaatcta
    22141 ttgatttgat tcctgtttgt aatggttcat aaacagacct tctatctatt ataccaggtg
    22201 cctcagattc aactggtcta gtttttgtgt acgctatagg tccttttcca tctattggct
    22261 gacctagtga gtttacaact ctacctatta aagcttctcc aactggaact tcaactattc
    22321 ttccagttct cttaactata tcgccttctt ttatttcact gtcatctcca aggataacag
    22381 ctccaactac ttcttcttcc aagttaagtg ccataccata tatttcacct gggaactcta
    22441 gcaactcacc agacatagct ttttctaatc catatacact cgctatacca tccccaactg
    22501 ttaaaacact acctgtatct gtcaactcaa ctttattctc ataatttttt atttgctgtt
    22561 taattataga acttatttct tcaggtttta agttcatggg ttcaccgcca ttcttaagat
    22621 attacttcag atagcttttc tttcatctta tcaagacgag ttttcacagt gccatctatt
    22681 tgttcatttc caattctaac tagaactccc cctagtatag tcttgtcaac aacattttct
    22741 atagtaacat ttttgttgta tttatttgaa agtttgactt caagttcttt aatatctgtt
    22801 tcactcattg gaatagcact tattgctact ccatcaatta cattgttttt cttatttaat
    22861 aattctttat aagctaattc gatatcttct aaatatgata ttctatcttt atctactaaa
    22921 acttttaaaa agttttttat gttagaactt acttctttac taaaaataat ttctaccaag
    22981 tttttctttt ctgattttga aactaatggt gattttaaaa ctttgtagaa atctttacta
    23041 actttagtca tcatatctac tactttttct agttctttga ataagacatc agttgaattt
    23101 tcttcttctc ctaattgaaa tagggcttct gcatatctat ttgctattac atttatcatt
    23161 tagcttcccc tacctctttt ataaagtttt ctatcaattc ttcatgttta gatttatcta
    23221 aatctttttc aatgacttta gatgctgcaa gtagtgctat gttagatata tcatttttga
    23281 tttcatttat aaccttttgt ctttcttgtt ctatatcttt gtttgctttt tcttttatat
    23341 caagagcatc tttctttgca gtatttacta tatcatctga tttctgctct gctcttatag
    23401 ttgcttgttt tatgatttct tgaccttcat ccttggcaaa attgattttt gactcatatt
    23461 cttttttcag agctaaacct tcattttttg ctttttcgcc ttctgccaaa tcacttttaa
    23521 tatcattttc tcttgattca attatattta atactggctt gaataaaagc tttctcagaa
    23581 gtaaaaatat tataaaagta ttaacaattt ggaacacaaa ttcccacgtt atacctacta
    23641 gtgctttttc cataattatc ctcctttcca taaatcataa ctacttataa tcacgagatg
    23701 ataaagttca tctcatgatt ataagtttga gatgatttac ctataaacct atctcaaaat
    23761 atttaaggac taaatctaca ttcctaagta tttaaagaat ggatttacga atagaagtat
    23821 tattgatata accaaaccat atattgctga agactctgct atagcaactc ctagtaataa
    23881 agttgatgtt atatcacctt tagcttctgg ttggtttcct actgcttctg cagctttagc
    23941 tgctgctata ccttgaccta tacctgcacc tatacctgtt gcaactgcta tccctgctcc
    24001 tattgctgat gctgccgcta ctattgctgt ttccataatt atttcctcct taaaacttta
    24061 attttttaaa ttattttatt tttatcttaa tataagttga tttcttaatc taactctcct
    24121 gcacttgata taaataccat tgttaacatt ataaatatat atgcttgtaa caatcctgca
    24181 aataagtcaa agtacaagtg taatgggact ggtattaata tttctccaat tggtattgat
    24241 aatcctggta ttaaactaga tataaaacct aaaagttgat aaaacagacc cattattatt
    24301 gttccaccta aaatattccc aaatggacgg aaacttaatg atattggtct agctaactca
    24361 cctataatat tgattggtaa cattatggca agtggttgag ccaattcttt aaagtatgat
    24421 aaccccttaa atttaactcc tgcaaaataa atcataaaaa atgtaatcat tgcccatgca
    24481 gcagttgtgt ctaagtcggt tgttggtgac cttaatccaa gtaatgggct taaatttgaa
    24541 catgcgaaaa ataaaaacaa gcttcctatg tatggtatca tatatggcat tctttttact
    24601 gtatttttac ccataacatc tgtaaccatt ttagcaagac cacctactgc aaattctaag
    24661 aatatttgaa ctttactagt tggaactttt tttaagtttc tagtcaaaaa ataagatgct
    24721 atcgctaaaa aggccattat tccccaactt actagtattg tttcactaat cttaaagttt
    24781 cctatgagat aataatgcgc ttgattcata cttatgcctc cttcctcttt aatttattta
    24841 taaagttttt tgataatatg acaaactttg tgctcaataa tcccaatgct gttccgatta
    24901 tatttaaatg tggtactgta gctgatgcaa ttagaacaac tgccattata aataatctaa
    24961 cagcatattg tgctgatgca taagcttgtg ccttcccagc aggaaatgtc actgattttt
    25021 ccagtgatat cgccaataat ctaaaattta acatagctat tatagagcca aatataagtc
    25081 ctaataatac tggtttactt aatgttgatg ttatcaacat tagcacaatt aaaactacat
    25141 caaatattac tatacctttt gttactgacc ttacctggtt aagtaatttt atatccaata
    25201 tatcacttcc ttcgtgtata tactattaga gttttataaa caccactaaa agcacctcca
    25261 acacctaaca ataaaaatat tattgtcaga actggagtag tattcaattt actatctaaa
    25321 aattttccta agaaagtaca tcctaatatt gatattacaa tcatcaatcc tatttgactt
    25381 attaaactaa acaactttgc aatttctgta tgtgtatttc ttttactcat aaatagtatc
    25441 cctcattatt ataaaataaa taacatgtaa ttaatctatc tcataaattt atttaattat
    25501 aaaatatatc ttactactaa tctgatgatt tttcaaatac tttcttttaa tcatttgata
    25561 ccattgtatt tcagtgtcat aatttgacat ttattgtcat ataaatttta tccactaaaa
    25621 ttttatgagt tctattcctg cttcttttaa taattctgta gacatatcat ctggataatc
    25681 accttcataa acaattttca atatacctgc atttatgcac atttttgcac acaaaacaca
    25741 aggtcttgta gtaacataca aagttgcccc atctacacta ataccattat gagcagcttg
    25801 tattatcgca ttttgttcag catgtaatgc tctacataat tcatgccttt ctcctgaggg
    25861 aatatttaat tcctgcctct tacatccaat attttcacag tgttcaagat ttctcggaga
    25921 accattatat ccagttgtta atatttgttt gtctcttaca attacagctc ctacttgtct
    25981 tctaatacaa gttgaacgct ttttcactac ttctgcaatt tccatgaagt attcatccca
    26041 tgatggtctc ataatttact tttctcctct ttgcttatgt actggtatta tatatttatt
    26101 agatttcaac ctaaataaat acttattttt atattatata cacatgttaa ttgaatgtcc
    26161 aataaaaaaa gagcattttt accatttaaa ttttaaatac ccgtcatatg ctctctttaa
    26221 tctattaaat ttattatatc atcttaataa aatattaata gctttatcta atataaatta
    26281 attttgggga taaatcccat tgtttagtat ggcttctaaa acccctccag ctatacatct
    26341 agctttatct gatattgtaa aagcattctt aacttcttct tttctagggt ctatgtctag
    26401 tatttttaaa tttttgttta cctttgctcc atcctttaat aaacctctaa ggactccaga
    26461 tattgttgct ctaacttcat gttttttttc ataattatta atatagcaaa gtaggtctcc
    26521 cttttcaact atatcaccta tcttacatac attttccatt atgccatcat actgtgaatg
    26581 cataactctc tctttagaaa aaccttttat tgctcctggt actcctgtat ttttttctgc
    26641 tctaccatta gttattattc ttcctagatt atgacctctc attgtctcta taacaacatc
    26701 tacatcttct cctgcacaaa atccaggacc taaagctatt gtaagtggtg ccatatcttt
    26761 agttgtacct aagtttttct tagcgagtat tccatcaacc aaaacgattg gatttaattt
    26821 agataatttt tctccttttg ggtctataat caaggctact ttatgttgag ataaaacttc
    26881 ctctatttct ttttcagtat tgcaaaatat acattctaca ccttcaacag ttactttttt
    26941 ttcataaaca gcctcacaaa aagaaatttg ccttcttata gctaatggct cttctatttc
    27001 taacgcaact actttaaaac cactgttgtg taatttatga ataactgctg ttgctaaatc
    27061 tccagcccct cttactacta tacaattctt catgtattac cactcccaat aatattatta
    27121 atattcaatt ctcaatttaa ctaatacact taatacctat aatcgtaagc aactaacatg
    27181 ccacattaaa atagactact attctataaa accttgtaaa ataagcaatc ttttttaatt
    27241 ggtaaaaact ttttattctc aattcgagaa atttatcaat tcaataattc ctattttgag
    27301 aaaaaatata taaaaaagaa ccttcttaca taaaattata tacactatat aagaaagtcc
    27361 ctaataaata tttatagtta tttattttta tttagttcca aacaatctat ctccagcatc
    27421 accaagacca ggaattatat atccatgttc atttaatctt tcgtcaactg atgcaacata
    27481 tatatcaaca tcatcatgat atttttgcac ttctgctata ccttctggtg cagctactaa
    27541 attagctaat tttatacttt tagctccttt agacttaagt aaatctatag ctgctacagc
    27601 agaaccccct gttgcaagca tagggtcaac tactattata tctctttctc ctatgtcttg
    27661 aggaagctta caataatatt caactggttt taatgtttct gggtctctat atagtcctac
    27721 atgtccaact tttgctgctg gcattaagct cactaatcca tctaccattc caagacctgc
    27781 tcttaaaatt ggaattattg ctaatttttt acctgcaact actttagatg atgttttttg
    27841 aataggagtt tctatttcta catcttttaa tggtatatct tttgttattt cataccccat
    27901 taacattgca atttcagtta aaagctctct aaagtcttta gaaccagtat ttttatctct
    27961 cattaaagtt aatttatgtt gtattaatgg atggtttgtc tctaccactt tgctcatttt
    28021 gataattcct cctgataaaa ctgtaatttt tattatttat tatgtttttc ttctattgat
    28081 ttaatcatgt ttactcttct agcatgtcta tcaccttcaa aatccgtatt aatccatgct
    28141 tcaactatct caagtgctag accttttcct aaaactcttt cgcctaatga taacatattt
    28201 gcatcattat gtgcttttga catttttgca gaaaatacat ctgatacaac tgcacatctt
    28261 atcccaggaa ccttattagc agctaaagat attcctatcc ctgtaccaca acataatatt
    28321 ccataatcta cttctttatt tataactgaa tttgcaacta tttctccata aacaggataa
    28381 tcaacagaat ctgtagcatt attagttcca taatctacac attctatacc tttactttct
    28441 aaatagctta ttatttcttt ttttaaatta tatcctccat ggtcacagcc taatcctatc
    28501 ttcatttgtt tatcctccgt ttcatatttt ttttactatt tcgttgacag aatacattat
    28561 ttctctcata gtgcttctat aaatatccaa atttccacca aatgggtcag atatatcttc
    28621 ctcattttta tttgcaaact ctttaaaagt atacactttt tctttgcatt ttggaactgc
    28681 ttgtactaaa atttctttat gagatttagt cattgttagt attatatctg attcatcaat
    28741 taatttttta gtaattactt tacttctatg atttgataag tctataccga tttcttttag
    28801 tacttctata gaattttcag aagcacccat tccatttgct gtagatatac ctgctgatga
    28861 tatagaatat tcttcaatac ttcttccaga ttctttaatt gctttcctta gtatagcctc
    28921 agccataggg cttctacaag tattacctgt acacacaata agtatattca tattttacaa
    28981 ctccctctta agcctttatc actttgtatc ctgctgactt cagtagtcta ttcataattg
    29041 cttgtccaac tccagtcttg gggaactctt ctgaatatat gacatcaaca ccctttttgt
    29101 ccatttctat aagagcatca aacagattac ttgcaacttc ttcaagagag tttcctaaat
    29161 cgattacttc tccatcataa aactctcggt ttctactaat acacattaca cctgtcttta
    29221 gacctttgtc tctattagat tttatcatat cattaatctt tgttgctaca ttttctcttt
    29281 ttccagatat tatatataca tctgcatttg gagaataatg tttatatttc attccaggag
    29341 ctttagcttt ttgattatct tcttttttac ttaatgatgg gtcaagcttt acttctccaa
    29401 gaagttcctc taaaacttct ttagttatac ttcctggtct cagtatcata ggagtctctt
    29461 ctgttaaatc caatacagtt gactccaatc caaaattact gtctcctcct aaaactattc
    29521 catccactct tccattcatc tcttcatata catgttttgc ttttgttgga gatgggcgac
    29581 ctgagatatt tgcagaagga gctgcaatag gtataccagc ttcttttatt attgccctgg
    29641 caataacatt agaaggcatc cttattgcaa ctgtatcaag ccctccacta gttttgtatg
    29701 gaattatatc ttttttattt aatataattg ttataggtcc tggccaaagc ttttctataa
    29761 caagtttagc tttatcactt atatctttgg ctaagtcata tacttcttct ttttcatata
    29821 tatggactat caatggattg tcactaggtc ttcctttagc ttcataaatc ttctttactg
    29881 cattttcatc taatgcattt gcacccaacc catatactgt ctcagttgga aaaataactg
    29941 ttttaccttc tctaagaagt tttgcttgta tttttatttc ctcataatca atattattta
    30001 tatcaatatt acttataata gttttcatct tttgtatgcc tgctttcatt ttaattttat
    30061 atataattta caatcattgt acctaacaag aaaccaatta tacaatacac actttttgaa
    30121 tgtggaaaaa tttcttccaa tacaacatac atcatagtac ctcctgctgt agcaagaaat
    30181 actcctataa gagaagtaaa tactcctcca aaataaacac ctaaaaaact tcctagaccc
    30241 ataggtaatc cagctattac agtaaacaaa ataactttac ttagtttcat tttattgcaa
    30301 actaagccta atgccatagc taacccttct ggtatattat gtagacctat aactatagca
    30361 agtgtaatac ctaaactttc agttgacata aaagaagaac caatggctag tccttctggt
    30421 aaattatgta gtaatatact tatgaatata agatatccag atgccatatt tccagacaca
    30481 tctaattttg tttttatgta catagttatg agagctccaa caaacgtaaa tataactgta
    30541 ttgattatcc ccattttgtc catggattct ttcattaaat caaacaccac tacagccaac
    30601 attattccac cagataatcc cataaaaaaa cttagatatt tatcgacctc tcttttaaaa
    30661 atggctgata ttactcctcc taatcctgtc cctataacac ctgccaaaag cccaattatt
    30721 gttactttta gtatcatata accctcctat acttgtatat aacaattata tacaatagaa
    30781 tatgataaca tgacctaaaa tgcaaaatag ctgtttcaaa atagaaaata atttctagga
    30841 agaaacagct tctttaaaac attacttata ttgcagtcat tttttctgtt tggtcaacag
    30901 taattaaagc atctatcatt tcatctatat ctccatctaa gaatgcatct aacttatata
    30961 aagttaaatt tattctatgg tcacttattc taccttgagg gaaattatat gttcttattc
    31021 tttctgacct gtctcctgtt ccgacttggc tttttctttc tgctgctata tctttgtgtt
    31081 gttctgccaa tgctttatcg taaagtctag cttttaatat ttttaaagct tgctctttat
    31141 tttttaattg tgattttcca tcttgacaag atactacttc acctgtaggt atatgtgtta
    31201 ccctaacagc agagtcagta gtattgacac tttgtcctcc attacctgaa gaacggaaaa
    31261 catctattct taaatcattt ggatttattt caacctctac atcttcaact tctggtaata
    31321 ctgcaacagt agaagttgat gtgtgtattc ttccaccaga ttcagttgat ggtattcttt
    31381 gaactctatg aactccagat tcatacttta atcttgaata agcaccttta ccttttatca
    31441 taaaagatac ttctttatat ccaccaactc cagtatcact tgcactcaat aattctattt
    31501 tccatcttct tctttcagca taccttgagt acattctaaa taaatcacca gcaaatagag
    31561 ctgcttcatc tcctccagct ccacctctaa tttcaactat aacgttctta tcatcattag
    31621 ggtctttagg caataatagt atttttattt cttcctccag aggtgctact ttttcttcca
    31681 tctcagctaa ctccatttta gctaattctc ttaattcttc atctgactct tcttgtaaaa
    31741 tttcttttga ttcttttata ctatctaaaa cgcttttata ctctctgtat ttcataacaa
    31801 taggttccaa gtcagcatgt tcttttatat atttctgcca aactttttga tcatttatta
    31861 catccggatc acctattttt tcacttaagt ctttatatgt atcttccaat acctctaatt
    31921 tttttaacat actttcacct cgattaaatg taacatatca attgttgatt atatcatata
    31981 ttgtaaccta taacaactct atcaatgcct tgaatatcct tctttgtgta tattttttta
    32041 tatccattac ttttcataat atttattaca tcttcagctt gattatgacc aacttcatat
    32101 gctaatattc cacacttatt taaatatttt ttaccttgtt ctgttattct tctataaaag
    32161 tctaatccat cttctccacc ttcaagagca ttatatggtt cataatcttt aacttgtttg
    32221 tgcagagttt ctatatcttg ttttttaata tatggtggat ttgaaactat gatatcaaat
    32281 tttatgttac tatcacttat tgctgtaaat aaatcagaat taatatactt tattttttca
    32341 ccaacttcat ttataatagc atttttttta gctatttcta atgctgtctc tgatatatca
    32401 aaagacatga ttttagaatt ttcaatgtac tttgctaaac ttattgtaat agctccagaa
    32461 ccagttccta tatctaatat acttacatct tttttttctc tacatatttc aattatttct
    32521 tctactaaag tttctgtatc tggtcttgga atcaatacgc cttccttaac aaaaaaatct
    32581 aatcccataa actccctatt ttcaactata tatgctattg gtcttccatt taatctttct
    32641 tctgcaaaac ccataaattt tgttttttgt tcttcagtta gctctttatt cagatttaag
    32701 tgaatatata atctatcaac acccaatgtt ttttgtaaca ataactctgt atctaatctt
    32761 ggagtatcac ttatatcttt tagtttatct gaatatttaa taattatatc ttttatcgtc
    32821 ataacaaata tccttttctc taatctacta ctgctttcaa tgcttccaaa gcaacctcta
    32881 attgctcatc atcaggctct tttgttgtaa aatattgcaa catcattcct ggatatgcaa
    32941 taattttagt aaatccatta tcatatttcc caagaactct aattacctca taagataaac
    33001 ctgctactat aggcacacat ataattctca taaatattct aagaattgga tttggccaac
    33061 caaaaaatgc aaacaatatt attgatacag ccataactat aaataggaaa tttgtaccac
    33121 atctaggatg taatctttta aattttctag cattctctac tgtaagttct aaatcatttt
    33181 catagcaata tatagattta tgttctgctc catgatattg aaagactctt tctatatcct
    33241 tacttcgtga aatcaatact atatatgcaa ataatatagc tattcttatg attccttcta
    33301 ttaaattaag cataaaatca ttaggcatta ctttagaaaa agctcctcct accaatgtag
    33361 gtataaaaat gaataatcca gcagaaagta acattgctat gaccaatgaa aatattataa
    33421 ttatatcatt tgccttatct ttaaaaattt tctttataaa taaatcaaat ttatcctctt
    33481 cagcttcttc cataaaaaac tctgaagaaa agtttaagct ttttatgcct tgaatcattg
    33541 tatctatcat aacaaaagaa cctctaacaa aaggtatctt atctatattc ttatctctta
    33601 cccaactttt tattctatct tcttttagtg ctatttctcc gtcacttttt ctaacagcta
    33661 ccgctctttt gtcttttgac tgcatcatta caccttcaat aacagcttgc ccacctacag
    33721 cttgttttgc catccactca cctcatttta ttgtatttag aatactcaat tttacatatt
    33781 tattattata atacaaaaag acaatttaaa gtagaatatg aatgtttttt tgtaataaaa
    33841 taggagttgg cttcctccaa ctccactttt ataaaaatta gtccattttg aatcttttct
    33901 tgaatttgtc gattcttcca cctttttcta tgttcttttg ttttcctgta tagaatggat
    33961 ggcactctga acatatttca acttttattt cgtccttagt tgaaccagca acaaatgtgt
    34021 tcccacaagc acaacgcact tcaactggat tgtattttgg ttgtatttcc ttttgcatta
    34081 tagtcacctc ttctctttat ttaaaaaaat taattatatt aactttcttt ttacattatc
    34141 aactacatta ttatagcaca acttattatg ccattcaagt gatttttcta ttagtttata
    34201 taatccatat ttgcatttgt attattgatt ttacaaattc tttattgatt ttacaaactc
    34261 tttattgtcc ttagttctct ttataagttc aataacttta tctgtaattt ccattactga
    34321 attattactc atttctcttc ttaatctcca aagagcagtc ttttcttcat catcaagcaa
    34381 taaatcatct cttcttgtac cagacttgta aatatctatt gcagggaata ttcttttttc
    34441 tgctaatttt ctatctaaat gtagctccat atttcctgta cctttaaatt cctcaaatat
    34501 tacatcatcc atcctagaac cagtttcaac caatgctgtt cctaatattg tcaaagaacc
    34561 accctgtcta atattccttg ctgcaccaaa gaacttttta ggtccatgaa gtgcaccagg
    34621 gtcaatacct ccagataaag ttctacctgt aggagatatt gtaagattat atgcccttgc
    34681 caatctagtt atactatcta gtaaaattac tacatcttta ccatgttcaa ctaatctttg
    34741 agctctattt aacaccatct ctgcaacctt gacatgatga cttgatactt ggtcaaaagt
    34801 agaatatata acgtctcctt caatagattc tttcatatca gtaacttcct ctggtctttc
    34861 atctataagg agtactataa gttcaacatt tggatgattt ttagctatgc tatttgcaac
    34921 gctttttaaa agaactgttt tcccagcttt tggaggtgct actatcaatc ctctttgtcc
    34981 ttttcctata ggtgaaatta aatctataat tcttgtagct atttcatttc tattagtttc
    35041 cagtgtaagt cgttcttctg gaaatattgg tgttaatgtc tcaaacgcat ttctttgtat
    35101 agctgtgtca gggttttcat catttatttt ttgaacatat aaaagtgctc taaatttctc
    35161 tccacttttt ggatgtctag ttattccctt aatcttgtct cctgttttca tattaaatct
    35221 tcttatttga gatggtgaaa cgtaaacatc tccttcagta gacaaataat ttgaacctct
    35281 taaaaaacca aatccatctg gcagaatttc aagtacacct actacttcat cctcttttga
    35341 tgtgttaaat tcatctacta tcttagattc atcaacttgt ttaggcatat aatagttttt
    35401 attattattt ctgaccattc tattagatat atttgatgac ttattgctat catttacact
    35461 cgttgtctta ttatagtcat ttctttgatt tctatgttct tggttttggg aaatattttg
    35521 ggtatctatt tctttcttgt tgtatgattt ggtagggctc ttatattcaa cttctttttc
    35581 tatactttca cgctctatat tttcttttct attctttatg tcttctttaa tatcttcatt
    35641 tttttcttta gtctcttcgt ttactttttc ctcattttta cttttggaat ttataatctc
    35701 aattaactca tttttcttat atgtagtaat tgattttatt tttaattctt tagctatctc
    35761 tcgtagctca actaaggtct tactacctaa tccttctaat actttatcat ctaaaatcaa
    35821 aaaaatcctc ctcatctata tattcattta tatacatact tattttggaa attgaaggca
    35881 tgaaaaagaa aagttttatt taaacagtta agtctaataa tatcattatt tatatattat
    35941 ggtcaataaa taaaatttaa aacattatta cctactatat tactgtattt caagtccaat
    36001 cttagcacgt ttttataaat ttatcactat atttatatct tataataata ttttccttta
    36061 aaatatgcaa ctattataaa aatttttttt tacatttata aattctaaaa accatatata
    36121 actaaaatat tgtaagtttt tgactacatc aagttatata tggtttttat atttatgtat
    36181 atcaactgct gtcatttatc ttattacata gttgcaatca tactcaaaac agctgttata
    36241 ctcgttatta cgaggattcc cacaatccca acagctatta ttctattaat tcttttctga
    36301 cttgattttg ccaccataca tcacctttca ttaattattt ttgaatttca agttctctag
    36361 cttcattgac ttcaattaaa tctatttttt catctgtatt attgtataac acattgttat
    36421 ttttatcaaa ttcactacat acatgtttca aatattttat aaaatcttcc ttaaggtctt
    36481 ctttctttag agcaaagtca acagtagctt ctaaaaatcc tagcttgtcc ccaacatcat
    36541 atctcttgcc ttcaaaattg tatgcataca tagcttcttt cttagataat atttttaaag
    36601 catctgtaag ttgtacctct ccacctttac ctggtggtaa atccttaaga atatcaaaga
    36661 tttcaggagt tattatatat ctacctaaaa tagcaatatt tgaaggagct tttcctgact
    36721 ctggcttttc taccatgtct tttaccttgt aaactctacc ttctatattt tttgcttcta
    36781 ttatcccata tttattagta tcttcttgat ttaccttttg aacacccaat attgtagttc
    36841 tatattcctc atatgcatct gttagttgtt ttagacaagg tacatcatta tctacaatat
    36901 catcccctaa cattactgca aacggctcat cacctataaa atgcctcgca caataaattg
    36961 catctcccaa cccttttggc tctttttgtc ttatataatg tatgttaatc atgttagata
    37021 tgttttgtac tacctccaac aattctttct tacctttttt ctctaaatca agttccaatt
    37081 ctacagattt atcaaagtgg tcttcaatag attttttatt tctacctgtt attattagta
    37141 tttcctctat tcctgaggcc acagcttctt ctattatgta ttgtagagtt ggcttatcaa
    37201 ctattggtaa catctctttt ggttgtgcct ttgtagctgg caaaaatcta gttcctaaac
    37261 ctgctgctgg tattacagcc tttttaactt taacttgcat agaatcatct cccactaatt
    37321 aataaataaa aagtcctatc ttgataggac tttaaacttt tagtatattt tatcattaat
    37381 tcgatatatt taccatgtgg attacaataa tgtttataat agcttttcaa gttctgctac
    37441 taaactatca aattttttga ttgtattttc tatgggtttt gtattagtta aatttatgcc
    37501 acttgatttt aacaagttta ttggataatc tgaacttccc tttttaagaa agctcatata
    37561 gttatctgaa ccattcttta ggatgtcttg ggcaagactt actcctgcac tacaacctgt
    37621 agcatattta tagacataga aactattata aaaatgtggg attctagccc atccgacctt
    37681 agataattgg tctaattcat agctttgtcc ataatatttt ttaagaagtt gtccccaaac
    37741 atcatttaat actaatgcgt ttacactttt tcctttttct actaactcgt gtatagtttt
    37801 ttcaaattca gcatacatag tttgtgtata aagagtatct tttatcaagt ctaaatactg
    37861 tgttatataa taagcttttt catcattgcc tttagcttct tttataagca tctcgtaaag
    37921 taatgcttca ttcgttgtag atgcaacttc gtgagtaaat atagacggat ttgaattaaa
    37981 ataattctga ttcttagaac ttaagtattc atatacagca tgtccaagct catgtgataa
    38041 agtcgaaact gaacctaatg aattattata atttaaaagt atatatggat gattttcata
    38101 aacagataag cagtatccac cacttacttt attttcatgt gaataaacat ctatccattt
    38161 ttcattgaaa gctttatata ctacatcatt gtattctttt cctaaaggtg acaacgctga
    38221 atatactata ctttgtgcct tttcataatt aatattatta tcaacagcct taaccatagg
    38281 cacaaacata tcatatgaat gaaccttatc taattttaat actttttttc ttaaatctat
    38341 atatttgtgt agactgtcca tattactatt tacagtcctt atcaaattat tgtatacttt
    38401 ttcatctaca ttatcagctt ccaggtacat ttctaaagaa gactcataac ctctttcctg
    38461 agaataaaaa atattctttt taacttgacc agtaattaaa cctgcaattg tatttatatt
    38521 atcattgtaa gttataagtt catcttgata agcttttttt ctattttctc tatctgatga
    38581 ctctaattca ctcgcatatt gagctggtgt taaattagtt tttctatcca tatttctaaa
    38641 taattcatac acttgacttg gaagactact tattgaagat actttgctta gaatatcttc
    38701 tgatttatca tccaaataat gtactttatt ccttcttata tctcttaaat atgcaccata
    38761 tttcctattt atatttttat ttgaaataat tttcttgtaa tctttgtttg ataattttag
    38821 tatttctaac tctaaatctg aacaaatccc catataattc ccataggatt tactcatact
    38881 atcattcata tctaagtact tatacgaatt tttattagta tcttgtttta attttacata
    38941 ggcagaaagt ttatttagtc ttatatcaag tttttcttta atgttcaagg caaaagacaa
    39001 atgtgttggc gattttgtta ctttacctat gtaatttttc aactctttag tatctttatc
    39061 aaaactctta agttcttttt tccattggtc atcactttta aacaaagatt gcaaattcca
    39121 acatgttttt tcatagtcta tttcttgttt tttttcagca tcatcttcag tatcattatt
    39181 attcatgaca gtaattgctc tgtcattatt cttttgatat ccttggtcag cttttatact
    39241 tattattaac atgcaaaaaa tagaaataaa taaaggtatt attgctttgc acaattttct
    39301 attcataaaa agccatcctt tcaataagtt tacttaataa taggatggct ttttttagat
    39361 atattattca attattcata ctatataaat aaatatcgaa ttaatagtta tccacataat
    39421 aatcaacata ttcacaggtt ttctttactt agaaatttag ttatttcaac atgaatctct
    39481 tttgataata gcttttgttt tttctttttt tacattttat taacatttct gccatactta
    39541 tcaacaaatt ttgttgattt atatgttatt tttgttaata actatacttt tttcaaaagt
    39601 ctattaacat attatacaca agttatccac atcttataca cataatcttg taaaccttat
    39661 tttgtacata ttttcgtaac taaaacagtc atgtcatctt cgatttgtaa cgcttgtaac
    39721 tctaatgctc tatccaatat taaatttgaa atttcctttg gatttgtagt ctctatattc
    39781 tttaagaagt atattaacca attgtctcct aaattgttat ttcttcctgc atctacaatt
    39841 ccatcagaaa ccattataac atagtctcct tccttaactt ttacattttt tctatcaatt
    39901 tttacatctg ataatatacc cacaggtaat gatgatgatg atattaagtc aacttctcca
    39961 tcctctcttt ttatataagt agaacacgct cccatcttta tagtttctaa acaacctcgt
    40021 tttaaatcta aaattcccaa atccagagtt gaaaacatct cttctgaaga ttttaacaac
    40081 aacatattgt ttatagtatc aatgactatc tcatctttaa tttttgcatc tatcattttt
    40141 tccagtatat caatagtggc agaagattct tcatatgctt ttttaccttt tcccatacca
    40201 tcacttatag ccatcatgta tttaccatca tttatctcca tgtatgtgta attgtctcca
    40261 caaaggatat gcccatccct tgacattgct gaaacttctg taacagcctt atatttttga
    40321 gatttaacga atgttgcctt gcatttacct cctaaacagt gacatcctat ttttctaaca
    40381 gacatatttt cacctactag gtctgaaatt atatttacaa ttttatcttc acacatacat
    40441 ccatcatggc aagttttctt ttctattgat atttcaaact cactgttact ttttgttaag
    40501 taacttactt tatcaactgt tatatcatat ctttctaact gttcatatat atttttttcc
    40561 ttttctatat ctagcattat gtcaccttct aaatcttgag ataaggattt aatggatttt
    40621 gaaatacttc ttatctgatt tgctattaac tttctacttt ctgaaaactt cacactccaa
    40681 tcataatcta atacaaacat tttgtaatag tgatttgata ttttcactat tgactctggt
    40741 ttcatacatt cctttctaaa atttttaggt atatcgttta gacttaactc ccctttctcc
    40801 tcaatttttt caagaatgtt atatatcata gtgtatgtat gattaaatct tgactcccag
    40861 cacattcttc tcatactaca acttttacat tcatcattat gaatcatatc tattacatta
    40921 gctatgtctc tttggtctag cactttgtct ttttctctta tcttatcaaa tgtatctgca
    40981 aggtcatcat aagttttata aatactgttc aatctgctat tagttaggtt tttgcttctc
    41041 ataatataat cgtatacgat ttcatttgaa gccacattac tttttattaa tttttcaatc
    41101 ttattaaata atcgctctgg caatactagt actatcaaac aacctaatag tatatctctt
    41161 aattgcatca tattagaagt aataccagaa gtatataggt atattattgt ccaacttaga
    41221 atatatccta gtatacagaa atatttattt attttattaa atgctccact tattaatcct
    41281 gaaaaagaat atatccccat ataaacagct gatgtcatat tatttaaaat ggatgctaca
    41341 ccaataatta caccactaga tgctcccatg gtagctccac ctactattga ggtcagcaat
    41401 atcaatacgg tcgataaaat ggtccttatt gatattccaa acagagatat atctcctatt
    41461 cccattatgc taaaggttag cagtaaactt attgatatgg cttcttctgt ccgtacagac
    41521 atcctattgt tgacattggc cattaaacct atcccataag aaaacacata tatagatata
    41581 aacattatta ttgcttctac tcctgctata agaagaccat atacatacct atttgaaaag
    41641 aaagcttgtc ccatggagat tggaagtagt attattgcac ctataaatgc aaccttagca
    41701 attgaatctg ataattttaa ttttttattc atagccataa atattgtaag gcacacagca
    41761 tattttgcta catttccaaa atcattagat gataacaata ttcctaataa tgttgatatg
    41821 aatacaggta tcttgtactt atcaatcttt gatgcacata taaaaaaggc aattcctaaa
    41881 ggagctacag agtctactat aatagaccta ctaagtaaaa atcccatcac agcgaatgcc
    41941 gccgtagtaa aatcaatata tccgcttatt aattttatga catttggttt tacagtctta
    42001 ttcttcacaa ctgctacact tctttgcata aaaaaacctc ctcttttgtt tatattaaga
    42061 attgtaacaa agaggagtaa ttttttttgt cataatcggt tttaaaaatc aaaaaatatt
    42121 aatacatttt ttttgacttt tgacatatta agccttccaa attatcgtat tcacttacgg
    42181 ttatttcttc tatttctaat ttttccatta ttatattcaa tatttttaca ccagcagtta
    42241 taatatccgc acgtttttct tgtagccctt ttaagttctt tttatcactt aatgtcatct
    42301 ttattaattt ttgtagaatt tcttctattt cttttttgtg tattttacta ttatgtattt
    42361 tttccataga gtaaatctct aattcttgat tcattgctga taatgatgta atcgtacctc
    42421 ctatacctat aagagttttt aatcctctgt cttttatatg atttaacgtt ttgtcaatct
    42481 catcatatat aaatgaactc atagcttcaa actcttcatc ttcaataata tcgtgcttca
    42541 aaaacctctc tgtcatccta agagcaccta cattctcact tttattaaat ttaattcctt
    42601 ctgaatcacc tacaataaat tctgttgaac ctcctccaat atctataaca ataatagtat
    42661 catcaccttg tattccttct aaaacaccct taaagcccaa atttgattct tctattcccg
    42721 taactatatc aacatctatt ccagtttctt ctttagctaa tttaacaaag taatctccat
    42781 ttttactgtc tctaagagca gatgtcccca tacaataaat tttttcacac ttttcatcat
    42841 tacacttatc agaaaattct tttaatgctt ttatatttct tttgatagca tcttctttta
    42901 tatatccttt actatccaca ccataaccaa ttctagttgt attaacaaat ttttttctgt
    42961 ttataaaatt cccatctcta taatctgcta tcagtaatct cattgagttt gtcccaatat
    43021 ctatagctcc tactttcatt atgaaattcc ctcctcaata aaaacaaaaa gccatcctta
    43081 actgaatgac ttttttatta aatcttaaat tttaaaatca atacatctgt aaacttatta
    43141 gcaagtttta atcaacgatt tatgtctaca taaactgttt catttggttt taccatattc
    43201 aatctttttc ttgctatatc ctctaagtct ccttcatatg atttttcatc tttctttaag
    43261 ctgtttattt gtatttcagt cttttttaat tgtttattta gacttgctat ttctttttta
    43321 tattccttag cttttgtata ctcaaataca aatcctgtca tcatactaaa aattgatatt
    43381 cctaaaaata aactgataac aataatctgt cctgaaaatt tttttcttaa gttcattttt
    43441 cctacaccct ttttttagtt ttattttttt tcttcttagg ttttgatttt ttcttgttct
    43501 ttaatttttt atttgatttt ctttttatac tttttcttgt tgctataaat atgtttggta
    43561 tataaaatat aatatcaaat agtaggtgta gtgaatagat tataacgtaa tacaaattat
    43621 ttaaaaaact gactatgtaa tgtgtaactt ttttaaaaga atttctcaca aaacgaatta
    43681 ttttattgat aattgataaa acaattttac ttatggtgtt atagtataaa ataaaaccaa
    43741 taaacaaagc cacaaaatga taatatctta aatcaaaaaa ttctgtaagg tttattgtta
    43801 cgaatattat aaccgtagct agaaaccaaa acaatacatc aaagattata gcaaaataat
    43861 tgatgaattt aaaattccct cttagccctc tataaaaatc aaacaacacc cctatgagta
    43921 ttccaccata aatagtagca taaaaaatgc ttacatcttg agtaaaaggt atcatctatc
    43981 taaatacctt tttcaagaaa ctctcttgtg gctttttagg ctttgcatat accatagaat
    44041 ttatagttcc atctatgcta attatatttt cgtctatact tagcttgctc atatctaggt
    44101 tttcgccttc aattaccatc tcgcctacag aagttttgag ctctactctt ttgtcattaa
    44161 aagagtatat gtgctcgact cccgaaataa ccagctttga tctatctttc aaagttatgt
    44221 tctgttccat aatatccccc cagtaaatat ttcaataata ctactattaa tatactagag
    44281 gactattaat tttattctaa aatttcgtac atttcttttg catcattttt taatacatgc
    44341 tcttttactt catttatctt aaacttcatc ttcttctctc caaattgtat ttctaatgta
    44401 tctccaatat tcacctctga agaagattta gcaacttttc catttatagt gacaatccct
    44461 ttatcacatg catctttagc aacagttctt ctttttataa tcctagatac ttttaaaaat
    44521 ttatctaatc tcataattat caccttcctt ggttctatag aaaaagtgtg gcaaatttat
    44581 ccacactttt tcacaagatt tctaattatc catttattat atcctttaat ccttttccag
    44641 ctttgaaaac tggtgcttta gaagctggta tatctataac ttgctctgga tctcttggat
    44701 ttcttccttg tctagcagct ctttctcttg tctcaaatgt tccaaatcca actaattgaa
    44761 ctttttcatt atttactagt gcatcttgaa cagaactcat aaatgcgttt aacgcagctt
    44821 ctgcttcctt ctttgttaat ccactttttt ctgccatctt tgatactaat tcagctttat
    44881 tcacgatatt tacctcctaa aaatgtttat aatatagact tctatctttg tgataaaagt
    44941 tctagatttt tgacaaattt ccttccacaa ttttattgtt ttttagcctg attccaaaac
    45001 tcatccatct cttcaagtgt catgtcttct aattttttat tcaagttcat tgcacttttt
    45061 tctataaagt caaatctatt tatgaatttt tctgtagtac agttcaaggc ttctcctgag
    45121 tctatgtcta aaaatctagc tagatttact atcgaaaata ataaatcgcc tagctcttcc
    45181 tttatgtatt ttatattccc gaccctgtgt tcgtcaagta attcctgata ttcttcttca
    45241 atttttttaa atacatcttc tacattgtcc caatcaaagc cgacaagtgc tgctttatgt
    45301 tgaactttac tagccttcat aagagctggt aaatgttttg gtatccttct aaggccatct
    45361 gttatagtac tttcgccttt ctcttctttc tttaattctt cccaagtttt ctcaaactta
    45421 gacatatcta attttgcacc actaaatata tgaggatgtc tatgaattaa ttttttacat
    45481 atgccattta ctacttcttt taaatcaaag tagccctcct catttcctat ctgacaatgg
    45541 aatataacct gtaaaagaac atcacctaac tcttctacca tttcatctat atcattatta
    45601 tcaattgcat tacaaagttc atatgcttcc tctataacag aattttttaa tgattgatgt
    45661 gtttgttttt tatcccattc acatccagat ggacttctaa gtgtattaac tattatttct
    45721 aagtcatgta ctgtattata catcttttta gaacttttgg gtatatataa gctagttaaa
    45781 tagtcaaaaa agttttcatt tctgtctaat tcacaaagct ttataaactt tttactttct
    45841 aaatctttga caccagctcc atttactata catacttctt gattatagtc ataatgttcc
    45901 ataagtttta actttacatt agatgctata aatttatcat atacctgagt tattatcata
    45961 cttgtatcca agtctatata tgaattttct atttcaaaag catctaccag tttaaatccc
    46021 tcagatgggt ctatagccaa atagttaaac atagcgtcta caaagctcat agaaggcaat
    46081 atttctactg ctatgtgctt ttcattggct aatctctcta ttatacttac agtcttctct
    46141 gcaactcttg gatgaccagg aacagcatac actaggtcac cctttgctga ttcttctatg
    46201 ataaattcag atattttcaa ataaacatta tcaaaatttt cctctttttc ataaaagtag
    46261 tctaaagaag tgtattgtat agtttcttta agtttatcaa ttattggatg tttctctgtt
    46321 ctaaaaaaaa ctttagggct atttattaaa gattctaaca caccttggct tataagagaa
    46381 tagtctcctg gtccaagacc tacaatactt atcttaccca taacatcact cctattagca
    46441 aatttcactt tatttttata ttattataca aataaggaga tttactattt gtatagtatt
    46501 tcctttattt gcatatatat tcctatatta tcttataagt ttaacttttt ttaacaattt
    46561 atataattta tcaccttttg gcatagttag aatctcttct tttttaattc cacctattcc
    46621 aagtattact actacatata caattccacc aacacatatt gcaattattg tagatatgct
    46681 gtttcctaag aatccaacta aagcaccata actcaactta accattataa acattgtcat
    46741 aactgttata aatggcttta ttataaattc ttttttagaa aagtttacat tcatgtgttt
    46801 tttaacatat agtaagttaa tcatggctgc cacaaagtat gctgtaactg taccaaatgc
    46861 tgaacccagt acattaatat ctggtatacc tgttaaagta taacttatta caattttaca
    46921 tagcatacca acacataaag caattacagg tactattggt tttcccattc cctgaattat
    46981 accagtcaat gtctgtatta atcctaaaaa taatacacat ggagtaagag taaacaaaat
    47041 agttcctaca gaagatggtt catgtggaaa taaaagaccc attattggta ttgctaaaga
    47101 agccatacca aacgcacaag gcaatactat aagtaaagtt accttaactg cactcttagt
    47161 atcttttcta gcttttgact tatttcctaa tgcatatgct ttagaaattg ctggaactaa
    47221 gctcatactc attgcagttg ttatgactgc tggtaaattt attgtggcca ttgccatacc
    47281 tgttaattga ccaaacatgg aatttgccac tttgtatgta aagcctgctt ccataagtct
    47341 tcttattaca ataacattat ctatcatatt tacaagtggc attactgatg ctcctattgt
    47401 tataggaatg gcaacagtta aaagcttttt aaaaatataa gacactcttt catctttaaa
    47461 tctttggctt gcatctatct ctgctctacg ctcttttctc cctagcatat aagcaaacac
    47521 caaataaagc atagatgcta ttgaacctat tgtagcccct gatatagcac ctgctgcacc
    47581 aaaagtctga cctagactct tcataagcaa gtaagccaaa gtaagaccta atacaactct
    47641 aaaaaattgc tctgcaactt gagacacagc aatcttagtc atatcttgtc tcccttgaaa
    47701 atatcctcta tatgcagaca ttgcaggaac aaatagtaat gctggagcaa tggctttcat
    47761 agagtataac gcacctggat ttttcattac gtttgttact atgtagtcgg ctccaaaaaa
    47821 taatatacaa aatgatatta taccggtaat gaataaaact gtatgtgata ccttaaatat
    47881 cttatttgca cctttaaaat ttccaattgc aactttttcc gaaaccaatt ttgccacagc
    47941 tgttggaaat ccagcagtag ctaatgttaa aaatagagta tacacaggat aagcagcctg
    48001 atagtatccc attccttctg aaccaatcat gtttccaagg ggaatcctaa aaaatccacc
    48061 cattattttt acgattacac ctgctagccc aagtataagg gctcctttta gaaaggagtc
    48121 tttatttttg ttattgttca taacataacc tccatactgt ttatataaca aactttaata
    48181 taccatattt ttaatgctat attcaatatt ttacacaaaa acttcaaaag accttcatgt
    48241 aaaaggtctt tatgataaaa ttattgaact tgttttccta aaaagttagc agcaattttt
    48301 aaagattttt cttcactttc tgcaattgta tctttatcct ttgaaacaac tacaattgaa
    48361 cctatacagt caccagatgc actaattata ggcatgataa cttgactagt atattctttt
    48421 tggtcttcct tgaataatgg taaaatgttt ttatcttttg aatattttac tgttctttca
    48481 cttattattg cttctaactc atcacttata cttttttctt tataatcttt tttaggaagc
    48541 ttagacacag ctattataga atctaagtct gttatcaaca ctccatatcc tagtacttca
    48601 gctaatgttt cagcatattc ttgcgaaaat tcatttaatt cacctattgg agagtatttt
    48661 ttaagtatga cttctccatc ttttgctgtg aatatctcta aagggtctcc ttctctaatt
    48721 cttaatgtct ttcttatctc ttttgggata actacccttc caagatcatc tattcttcta
    48781 actatacctg ttgctttcat attttttaat ccctccataa attactttaa gtttattttt
    48841 accattgtta agaaaaatat acaaaaaaag ctatatctta tccagatata gcttttttaa
    48901 gcatattact ctttttttaa gtcttagatt gtgattttat ttattacttt ttcatctttt
    48961 tctaccttag cttctttagt taatttttct atttgttctt gatatttatt tttcaacagt
    49021 tggtctttta tagtttcttt agcatcttca aatgatgttt gctcatttat tctgtctgtc
    49081 actttaatta tgtggtatcc atattgagtt tcaactaaat cagatacttc accctttttc
    49141 atagaaaaag cagcatcttc aaattcagca accatttgac cccttgaaaa gaatcctaat
    49201 ttaccaccat cacttgctga agtatcttgt gaatattttt ttgctacttt tgcaaaatcc
    49261 tcacctgatt taacttcttt aagagcctct tcagcctttt tcttagcttc agctttttcc
    49321 ttatctgaaa gtggcttgtt attatcatct actgttttca ataaaatatg agaagcttct
    49381 acctcatctt ttttaaattc atctttatgt gtatcatagt attttttcat ttcttcatct
    49441 gatattttag tctttttagc aaagttactt tgatagtttt gcattgctaa atctttctct
    49501 tgttggtctc ttaaaaactc atcatctata cctaatttct taagttgttc tttatatttt
    49561 tcatctttac ccatagcgtc ttttaattct ttaaagcttt tttcaacatc ctctttttta
    49621 ggaagtaagt tatctttttt agcttgagaa tatattactt ctgtagttat aagttggtca
    49681 agaattaaat ctttaaactt gtccttgtat tttacaccct tttcaacttc cttatcccat
    49741 atatcttttc cataagtttg ttccatagaa tctttataaa gagctatagt ttttttaaat
    49801 tcatctgatg atatctttgt accttcaact gttgccacag tttctccttt actagaactg
    49861 caagcagtca cagatactac caaaatcata gctattacca atgttattac ctttttcaaa
    49921 tttaaaacat cccttcttaa atttacaagt tattttacct tgcacatctt ttctaataat
    49981 tcttctaatt ctgtaactat attataacct attttctgtt tagttctata ctttgttatt
    50041 ggaactaata atatttcatc ttttaattgt cttatctttt ctatttttaa tatcttacat
    50101 aacgatttta tataagctat tgtaagaagt gtctggactg gtttaggtat atcagagaat
    50161 ctatcctcaa gttcttcttg tatatccaac atgtcttctt tgttttcaat agaagctatt
    50221 ttcttataca tttcaatttt aatcaactca tctttgatat aattatctgg aatgtatgca
    50281 tttacactta aatctatttc aacatcaatt tcctccacaa taggttcacc tttgactttt
    50341 ttaattgcat cattaagcat tttaacgtac aaatcataac ctataactgc catatgacca
    50401 tgttgttgag aacctaaaat atttcctgct cctcttattt caaggtctct cattgctatt
    50461 ttaaaaccag aaccaaactc tgtaaattct ctgatagctt ttaatctctt ctccgctatc
    50521 tcacttaaag ttttatcttt ttcatacaaa agatatgcat atccttgtct tgaacttctt
    50581 ccaaccctac ctctcaattg gtaaagttga gccaatccca ttttatctgc atcatatata
    50641 atcattgtat ttgcatttga tatgtccata ccagtttcaa ttattgtagt acagactagt
    50701 acatcataat ctttatttaa aaatcctagg attatatttt ctaatgattt agaagtcatt
    50761 cttccatgtg caacagccac tctagcatct ggtacgagtt tttgaatcat acttgccatt
    50821 tcttcaatgt gttcaactct attgtataca aaaaatacct gtccacctct acttaattct
    50881 ctctctatct catcttgaat tacgctttcc ttactctctg taacatatgt tattacagga
    50941 tgtctttctt gtggaggttc ctctataaca ctcatgtctc ttattccact taaagacata
    51001 tgtaatgttc ttggtatagg agttgctgaa agtgtaagta catctactgt agatttaatt
    51061 ttctttaaag cttctttatg ttttactcca aatctttgtt cttcatcaat gactacaaga
    51121 cctaagtttg gtaaatttat atctttggat attattctat gtgttccaat taaaatatct
    51181 actaatcctt ttttagcatc ttcaataatc tgtttttgtt gttttggtgt tttaaaccta
    51241 cttaaaactt ctactctcaa aggataattt tcaaacctct ctttgaatgt attataatgt
    51301 tgttgggcca atattgttgt tggaactaat acagcaactt gtttttggtc catacaagct
    51361 ttaaaaatac ttctaatagc tacctctgtt tttccataac caacatctcc acatactagc
    51421 ctatccatta ccttacttga ttccatatct tttttagttt cttctatagc ctttaattgg
    51481 tcttctgttt cttgatatgg aaacaacgat tcaaactctg cctgccaagg tgtatctttt
    51541 gaaaacttat acccttgtat tttttcacgt tttgcataaa gctcaatcaa gtcttttgtc
    51601 atatcttcaa tttctttttt aacttttgct tttgctttcg tccactcatt tgttccaagc
    51661 ttatttaact taactttttc tacttctgca ccaatatatt tttgtacttt gtccatctgg
    51721 tctattggca catatagatt atctccacct tgatatacta ttttcatata atctttttta
    51781 atagcattga ctgtaatttg ttcaattcct gtgtatctac ctacaccact attttcatga
    51841 actacatagt ctccaacact taaatctaaa aaggattcta ttttctttcc ctttttaact
    51901 tttttattgc tagtcttaga cgctctttta taaacaccta tcatttcgtt atctgttata
    51961 acaacaaact ttatagattt atactgaaaa ccactgttta tgtgtgcagg tacaattata
    52021 acttgtgaag atttaatttc aatatctcta tctttagaaa tggtcgtttc taagcctttg
    52081 tccaataaat cttttcctaa tttatttgct ctttctaatg tatttgttgc taaaattatt
    52141 ttgtatccat tatatttcag ccgatttaat tcttctacaa gcaaatctac ctttccatta
    52201 aatgtaggaa cttctctact ttcaaaattt attatagatt ttatagaaaa gttatttata
    52261 gactttggca atagcgagtt taataccaca gacttgtctt ttactaagta ctctaaatct
    52321 gtatagtgat ataacaattt accttggttt ttaatagcca gacctctttc aagatttaat
    52381 ttataatttt ctctaaactc atttatataa ttttcgcatc tttctttaag tctcgttata
    52441 tcattgataa atactattgc atcatctgct aaataagtaa atatactttt gttttcttct
    52501 ggataaatat aatctatata gttctctaca ccttcaaaat aagtcttact tgatatataa
    52561 tctatatttt gaaatgtacc ttcatcagta tttttagttg tttctttttt taatttaact
    52621 aatgcatctg tagttttttc gggataaata aattccctag atggagttat agagaattgt
    52681 tgcaatttat caattgattt ttgtgaatat acatcaaaag ttcgtatgga atctatctca
    52741 tcatcaaata attccatacg aattggattt gtatattcaa gtgaaaatac atcaataatt
    52801 ccacctctta tactaaattg accaaacccc tctatttttg aaactctttc atatcccaat
    52861 gaaactaatt tctcagttaa cttttctaag tctaagctat ctcccacctt ataatgagat
    52921 acactatcga gcaaaacttg ttttggtatg tattttctta aaactgcttc aatagaagtt
    52981 actaaaatga ttttttcttt atttgctagt tttaacaata cttttaattt ctttgcttct
    53041 tcgtttctgt cctttgcatc caagtgataa aaataaatat cttgaaaacc taaatattca
    53101 actttatctt ccatataaaa acttaaatct tcgtatactt tctttgcttc taaatcacta
    53161 ctcgttataa aaatcatttg tcgtgataaa tcattaaata tagaatacga tatatgaggt
    53221 ttttgtactg gtaaaagtcc attaaccaat aatgagcctt ttgtattttt tatacagttt
    53281 attatatctt tatactcttt tgaattttgc aaaggatata aaaaaacatc attcatttaa
    53341 atctccctct cactatccat tatatttgtt catagacata tctatatttt ctcttattat
    53401 tgagtcaata gcatctgccg ctttttctaa tgcttcattt atgttatcag attcttcttt
    53461 tctaaatcta gaaagtacaa agtctgctaa gtcttgacca gcttctggtt tagatacacc
    53521 tactctcact cttggaaagt catttgaacc taggcactta gttatagatt tcatgccatt
    53581 atgagttcct ccacttcctt tttttcttat acgtatcttt ccaacttcta aatctatatc
    53641 atcatatact actactatat tacttaaatc aaccttataa tatttatata tatctattaa
    53701 tgtttctcca cttaaattca tataagtttg tggtttaact aataatactt tttcagtacc
    53761 tactctccct tcaccaatca aagctttatg ctttatttta gtaacactaa tattgtattc
    53821 tttagcaaga atatctataa catcaaatcc aacgttatgt ctagtcttct catacttctt
    53881 tcctggatta cctaacccta ctactacgta catttcttac ctcctgttat ataaatttgt
    53941 ataacaaatt ataataaaaa ttaaatatat tatactctgt aaatattatt aaaattgttg
    54001 acaaggaaat aaaaggtgta aaaggaattt gctttcttat atctacatct ttaaatattt
    54061 tagttatgta taaaataata aaaaacaaaa acgctaaaat aaatgataaa actatcacat
    54121 aaaacccata attatttccc aatgcaaagc aacataagct atagagacct acatctccat
    54181 agcctaatcc ttttgttagt ttagcaataa tataaggtat aataaagcct atcaatagtc
    54241 caaatatatg atttatataa tcaaattgaa ctatattgaa aaagaccaaa aaaattatac
    54301 cttgaactat tataccacta actattgtta tatcatatac atatattgtg tagtaatcta
    54361 caatggatat aattattaaa aatggaatta aaaccatata ttgtacacat tctacactaa
    54421 tcccaaattt tagatatatt aatacagata aaactacagt tattatgtaa ataaaaaacg
    54481 tattgtataa atttttcttt tctctatcac aaaaaacaca tagagattta tttatcaaat
    54541 gtgcaaaaaa taaggaaatt attattatat actccatagt ttgtagcact ttagtaatag
    54601 ggttaataat aatattgtcc ttattaaata ctttaattca tttttttaaa ttaaaacttt
    54661 atttaacttg actatcatct atataaccat agaaaaataa agttcaagta attgctttcc
    54721 acataagatt gttattaatg ctccaataga tataaaaggt ccatatggta taacagaatt
    54781 atattccttt ctttttattc tacttataat tattacacag attccataga ctgctcccac
    54841 ataaacgctt agtaaggctg tcaaaagacc atttttaaca cccatgtaaa aacctagcat
    54901 tgcaaatagt ttgatatctc ctccacccat acactctttt tttattacaa attcaatcaa
    54961 aagaactata atccagacac ttcctccaca aataaaaaca cctagaattg aatccttaac
    55021 tggaatttct ttaaataaaa ggttaaaaat catagaaaat atagctccaa aaaccaatat
    55081 tttatctggt attataaagt ggtcaatatc tataaatgta attataatca gtaaagacac
    55141 aagaaccaaa taataaatag ttgatgtaga tattccatat aacataaata ctgttaaaaa
    55201 taaaagtcct gtaattaatt ccactatagc atatctacta gaaattttct cataacaata
    55261 tctacacttt ctgccactca ttaaatagct taacactggt attaagtcta ttggctttag
    55321 tctagtatta caactcatac aatgagaagg aggtctagaa atagatatac cttctggaat
    55381 cctaaaaata cagacgttaa agaaacttcc aaacactata cccactataa atacatatag
    55441 attaacgatt ataatatcca ttgtagttta ctccacctat cattcttgtc ataaactctt
    55501 ggtcagtaca cctactaagt gccatatctt ttgtaatctt tccattttta taaagttcca
    55561 ttaaatcttg gtccatagtt ttcataccta ctttaactcc agtttgtatc atgtttggaa
    55621 tttgatatgt tttgccttcc cttatcaggt tttttattgc tggagtagca aacatcaatt
    55681 caattgctgc tattctgcct ttcccatctg ctgtaggaag taactgctgt gataccacac
    55741 cttcacaaac tgtagataac tgtgttttta tttgttgctg ttgggatggt tgaaacatgt
    55801 ctactattct atcaattgtc ttagctgccc ccactgtatg tagtgttgaa aatactaaat
    55861 gacctgtttc tgctgctgtt aatgctattg aaacagtttc agggtctctc atctctccta
    55921 ttagtattac atctgggtcc tgacgcaaaa tagctctaag ggctgaatga aaactttctg
    55981 tatcagttcc tacttctctt tgatttacta aactttgttt atgatgatgt acatattcta
    56041 tcgggtcttc aagtgtaatt atatgttttt gttgtttttc atttattata tttatcatac
    56101 ttgcaagtgt tgtactttta ccacttcctg ttggacctgt aactaaaact aatcctttat
    56161 gcttttcagt aaaagatttt atactctctg gaaggcctaa tttttcaaat gttggaattt
    56221 ctgctgttat agtccttatt gctattgcgt aatttccttt ttgcctatat gcatttaccc
    56281 taaatctttc tcctgttttt agagaagctg aaaaatcagc ttctccatgt tcttctatta
    56341 ctttaaacat agattccccc gctatgtctt ttgccatttg cattgttact tctgatgtca
    56401 atatttgttc acttagtttt acaaatccac cttttactct agctacagga tttgttccaa
    56461 cggttatgtg tatatctgat gcgtttagtc ttataccttg ctctaataaa tcataaatat
    56521 acataatatt tactctccat atcttctatt agtttactta tttttttagt agtaaataat
    56581 aaaattattt tattatttat ttatactaag ttgcttctta tatttagttt acttttttgt
    56641 atgaacttat atcatgatta atcaaaattt tatcttttat atcataattt ccaactacat
    56701 ttgtatctat atttagatta aagtttacaa tggcgtcaac cttatacttt gaatttgaat
    56761 tttcttcaac acctaaacca ataatatttt ttttaccatc tgtaacttct acttctttta
    56821 ttccatcttt atcaccagtt accattaagc ttaactccgc ccctgaacca ttttctttta
    56881 atttaggcca tccttctttt gtcatgtcgt agtcagcctt ataaaaaggt atttttatag
    56941 taataccttc tgttttatca atttcttgtg gttttaattc ttttaatctg cattttatct
    57001 ctgctaaaag ctcattttgt atatagtcaa aagttgttat caaagccatg ttatcatatt
    57061 taacaggtat tttctcataa gacatacttt ttagttgatt acattggtct gatatttcat
    57121 acaataactc tttatacttt aagaaatttt ttgctgtatt ttttttacca ctagatatat
    57181 tatcgcttaa cttctcaaat tttcttacat tatttctcaa atgaactctt aaagaattaa
    57241 atccttgagt ttgatctggt aaaatttcca ataggttttc taatgtatcc catttactat
    57301 tccagctata ctccatacta tttattatag gattttgtat tagttcataa tattgttgag
    57361 agtctggaat cccattagat acttctactt ttggctcatc tatatgaaac gtatgtctat
    57421 actcaatcat attttgcatc gccaaagaaa tgttatctaa tgccttataa gtatgtgaaa
    57481 aggctgaatt taaatgttca ttttcaattt tattttcttc taaaaacatt ttcaaaagaa
    57541 tatcattatt cactaacttt gcctcaatca atgtagttga gtctatatct tctttcagaa
    57601 tatattcatt tattttacta atatctccag aagtataatt cttgcaaatt tctaagatag
    57661 aatctctatg tgaaataata tttgcatcta catattcaat gtctctatag tccaacatat
    57721 ctaaatcata tagctttttc aattccatac tagctttttc caagtctgaa gaagctttat
    57781 ttaaatagaa atttatatta ttcatatacc caagtctttc tgcttcaata ttttcctcat
    57841 cttgattatt aaaatcttca tctgatatat ttcttaattt tgacctagat atggattttt
    57901 cttgattttc acttttaaac tcattatcca ttttctcaag ctcatcatac aatttttcat
    57961 atgctttatc aatactattt tccacacatg tctctgctaa atactttgct tgtatacctt
    58021 cattagtagt ctttgtagat tttattctat tagctatctg atttgaaaat atcaatccaa
    58081 caatagaaag caatgctact acaaccaaaa ctaaaactaa agctgcccct ctttcacttt
    58141 taaatttatt ccacttcctc aaaatctcac ccccaagtaa aattatttat ctttaaatat
    58201 ttcagttaca attttaacct cttttttact tgaatagtca ttcatgcttt tcccagttac
    58261 agctatttta tatatctgac cacttttatc ttctacaata gtctttatct tatttgtaat
    58321 atttcctttg tcgattaaat taccatcttc atcttttatt gttgtaatct tttcatatgg
    58381 agtatcatat aagctcacat caccttcact aagaacttta tcatatacaa ctttttttga
    58441 tttatctatt aatttttcat atacgacagt tccatcttct agtttatcta aactatcaaa
    58501 atcaaattct ccacttttta tcttttctct tagattttct atttcacttt ggacatagtt
    58561 taaggaagtt atatctgtgt catttttagt gtttgctttg ttattagtat tgataatatt
    58621 aaaaaatgca aatagtacaa ttcccataat agccatagct actaacactt caattaagga
    58681 aaatcctctt tcactatttt ttttactcat attttatcaa cctacttatt ttttaataaa
    58741 ttcttattat cctattcttg acataacatc aaatgtataa gttttgttat ttaaattgct
    58801 atttttatga ctttcttgca ctgattcatt atagtatata cttacagtat aaatagattt
    58861 accagtttgc ttttctattt taaatggtgt ttccttcatt tctttattgt tttgaactag
    58921 tggttgatta tagattatct cttctctaac ttcattttct gtatcaattg tattgttttg
    58981 gattctcgtt acagaatata cctctgtatt ttttttagtt tctatagaaa cttcatattt
    59041 tacaacatta tctggcatct ctatattgta ttcataatta ttacctgaac cagttttagt
    59101 tagctcttta cagttttcca aatctctatt tagatactta ttaattagat taatatttac
    59161 ttgagcatct gtaataatct gttgcttttt tactgctgat gttataccat tgaacacttt
    59221 ataagaaata cttaaaacta ttgtcagtat aaaaacagct attattactt ctaataaagt
    59281 aagacctttt tcattatttt tgtacaaact catcacctat ctttaaagct tacttagctt
    59341 tatttcgcac tagacttaaa tggattatat tttcctggtc tccatgaaga tggattatct
    59401 gtatcaagta tcattgtagt attctcttta gtgtacatta taaatttaaa tgtagcatct
    59461 actacacctt ctgcctttgg agctacgact tctttttttg cttctttttc tgttgcagct
    59521 ctaagtacac aagtattaca tagataatta gtagacctat ctaaaagttt gtcatttttt
    59581 agaatctctc cctcttttat caatggacaa tccttatata aatgccactt attttcgcct
    59641 tccacccagt atatttttgt atctttattt atgttcacat ttttaattaa atccttattg
    59701 tttgttgttt cttcctcttc tttttctggt tctatatatg aagtcattgt ataatctagt
    59761 atttgagtaa catttttttg ttcttctaaa taagccatta attttttgac atttctataa
    59821 tcacctctaa cacttattgt tgttgatata ccataaaaat gttcgtattc tttaacattt
    59881 ccaacgtcat aactggttaa gtcaatatta taagccttca tttttttatc taaacctatt
    59941 aaaaaaagac catcttgcat atcatataaa agatttttgc ttttttcttt taattcactt
    60001 tcaacttctt caagttgttt ttcatatttt ggcataagtg cagttttctc tttatatata
    60061 tttaattttt cagtatcctg ttttacagaa ttttttgtag caatatattt ctgatattga
    60121 gggtaaagtc caaaataacc atatgataaa atagctaata aaattataac ctttataaga
    60181 atccctttta gactaacttc cattttaaat atatctttta tatctttttt aaatatatct
    60241 ttattcatta gttaatacac cttctttcag gtataatgtc atactaaatg tatagttaga
    60301 atcttctgca ttacttttta tgctatgtaa tttaacatcc ttgaaatagt ataatccctt
    60361 taatttatat aaaaattgac ctacataaga ggaatcttta gcatttcctt ctatatttat
    60421 aacatttggt atcttatcat acaatggagt tttatcatct ttatctgtgt tattactatt
    60481 agtctcacta tttccagtta atgcttctga cacatttgta gtcttatctt catttaaatc
    60541 attatctgtt ttatctccat tactattact tttcgtactt gattttgttt cattgttact
    60601 tgatttatct gttgtagtct tattactttt tgaattatca gctttattat tagttccaag
    60661 attttgataa gttgttgata aacttgttag ttcaacttta cttggagtat ttttatctat
    60721 atcatccaat aaattttccc actttacctt tttcccaatt aacatatcat atacctttat
    60781 ttcttcgctt ttactctgta tcattttttc tatatttcca atctgataat atgcagattc
    60841 aatatttctt atttcaccat taacattttc aagttcctta tttgctaaat tatttgaaaa
    60901 aattaaacat aaataaggta tagcaagaat caaaataact attggagcaa ttttcagtat
    60961 gtttttcatt tgtttagctt ttatctgttt tctaagtata tccattggta aaaagtttat
    61021 attttcaaaa tctcttttcc ctttaggttc tatcatactt cctaaaagct ctacaattga
    61081 atttatatca tcttcatcta tatctccagt tatcatatca gatatatcca ttatctcagt
    61141 aaggccttga gaaacatagc ttgaaaatgc agagctaaaa gtttcataaa ttaatttatt
    61201 ggaatatctt tttcctaata ttaatatagt atccgtaacc ttaccaaagt ttcttgacga
    61261 atagtaattc ataagacctt ttgattcttc tactacattt aataattgca tgtccatatc
    61321 tttagcagtt atctttatag gtatattatc actaataaat aaagtatctt tcttatatat
    61381 atttataatt gtgctattgt cacttatatt ggttaccatt atatcattgt agtctaaatt
    61441 tttaagcatt cttaagtaag ctgttgataa tgtatctatt ctatctaata ctaaatcaaa
    61501 ttcatgcatt atctctacaa ttgcatttac ctcttccttt tgtatagctc caataataag
    61561 atttaaactt gattcatcgc catctgaatt atcaggtgtt tcactagata cttcatacga
    61621 aaatacatat tcatctgact ccaaagccat catttcgtcc atctctagct tcataaatcc
    61681 gtctaaatcc tttttgtcca tattaggaag tttttggggt tttaaaatta catctcttgt
    61741 atttaaacaa aaaactacat gtcttgattt taacttaaca ttttctaatg cgtatctcaa
    61801 tagagcatac ttttccttag aaaaatcttg tgcaatatcc tctattgaaa acacattaaa
    61861 attttttaca taaaacttat ccttatcgtg tttatatctg ccctcaacta tggaaataga
    61921 atcgccataa tatgatacat aaaccttgtt catttgtcct ttccctccta catggcatca
    61981 tatatactaa gcattggcat aaatattgat acaaccagta tcgcaactat aataccaact
    62041 gcaattatta aaacaggctc tatcattgag gccatcctat ttaaataaat ctcggattca
    62101 tactcataaa attctgccat ctgtctactt atattctcta attcccctgt ctgctcccct
    62161 atagctatca tctgtaccaa cacagagtca aaatacgtat atctctctaa attttctgct
    62221 aagccttctc ctcttgttat tccatcttta gctgatgcta aaatcttctc tgccatggtg
    62281 ttatttacac tatctataat ataatcaagc ccttgaacta atgggtatcc tgttgataca
    62341 aatagataca atgctcttga aaatcgtgta gctaccatac aatttattcc ttttcctaaa
    62401 acaggtatct tgtttataac tctatcttta tgagcccttc caactgacgt ttttaaatat
    62461 tttttaaact gatagataaa taatataata actaaaagta ctattatcca aaacttcttc
    62521 ataaacatac caaaccccat aacaattctt gttataagtg gaagagttgc tcctactgtt
    62581 gtaattgaat taatcatctt aggcaccata aatgttgtaa atataaaaat cattataaat
    62641 gaagaaatca aaactattaa tggatatgtt gcagcatttc taagcttttg tttaaccctg
    62701 tgttcacttg cataaaatga agccatactt tttaatactt catctaattt acctgtagct
    62761 tcacctgtag ctaccatagc accaagtaac tttggaaact ttgattttga tgaaagcatt
    62821 gaatctgcaa tagtagaacc tacttttatt tctgccacta tacgattaat ttcttctctt
    62881 aatgtaatgt tagttgacct tctagctaat gtttctaatc cagaaatact atttatacca
    62941 gaagatacta taatagcaaa ctgtctacaa aagtggctta tctggtcagc tcctaagtct
    63001 ttttttctct ttctactaga taatcttata tttttatttt tgctttcttt gatttcaata
    63061 actctaagat ttctctttct aagaagattt ctagcttcat taaaatcgtt ggcttcaatc
    63121 acatcagttt gcttttttcc gtcctctttt attactgtgt atttaaaact tttcataata
    63181 tcatccttta atcagtcatc aaaattattc taatcatttc ttctactgtt gtctttccat
    63241 ttaaaacctt tttaactata tcctctttta agctaaccat accttgtttt ctagccatct
    63301 ttaaaatttc tctttgattt gcagatgaat ctattaactc ttttatttct ggagtaattt
    63361 caagcatttc aaatatacct agacgtcctt tatatccagt attattacac ctttcacaac
    63421 cttttcctct ataaatagtt actgattcat ttacatcaat accaagagcc tttttttctc
    63481 caatatctgc ttcatatgaa accttacaat gctcacatat ttttcgtgta agtctttgag
    63541 atattacacc taaaacagat gaagttatta aaaaactttc cacatccata tctaaaagac
    63601 gtgaaattgc tccaactgct gtatttgcat gtaatgtact taaaactaaa tgtcctgtaa
    63661 gagaagctct aattgctatt tctgctgtct cagtatctct tatctctcca accattatta
    63721 tatctgggtc ttgacgcata aaagctctaa gtccgctggc gaaagtaagc cctgcctttt
    63781 catttatttg agattgattt acaccaggta attcatactc tatagggtct tctattgtta
    63841 atagatttct attttctgta tttagttgat ttaacattga atacaatgta gtggttttcc
    63901 cacttccagt agggccaaca accaatataa gaccataagg agtgtttatc aattcatcat
    63961 atatcttaga gccatgttcg tctattccta gtacttcttt gctaaccata aaattactct
    64021 tatcaagaac cctcatagct atcttttcat ccctgttagt tggcattgta gaaaccctaa
    64081 aatctatggg tttattatcc acttttaaat agattctacc atcctgtggt attctctttt
    64141 cagatatatt tatatctgac attatcttga tacggctgat tactgcctta tatggtgctg
    64201 agttcatcct catatattct cttaacattc catctatcct aaacctaaca cgcatgtaat
    64261 tttcactttg ttctatatgt atatcacttg cttccattct aacagcattt tctagtatgt
    64321 tatttactaa tcgtactatt ggagcagcat ttacatcttc ttcaagtatt actgtttctc
    64381 ttactccacc agttctattt ttgttgtact cagtcattgc cttatttatt tctgaatgtg
    64441 cataagcatg acctattgct ttgtttatat cttctgtact tgatataaat atctctacgt
    64501 ctttacctga catacgcctt acatcttgaa ctgcttctct gtcttgtggg tctgacatgg
    64561 ctagacatat tttatttcca tcaattttaa atggaaatac agtatatctt ttgcatatat
    64621 tttctggaac catttttgta gctaaagtgt ctatttctgt cccttctaaa aatatacttt
    64681 ctatatccaa gagttctttt aatacactta ttagtaagtt actatctata agcccttctt
    64741 gtactagaaa ttctcctagt ctttttccac tgtttttttg ttctgatagt gcccatttaa
    64801 gctgttcttc tgttatatat cctttttcga ccagtttgtc tcctattcta acttttttag
    64861 ccacaggttt cactccttta ctatctcaat tatatcatca ttcataattg aacattaaag
    64921 aaaatagtat aaaaaaacag gaagattttc ttcctgtctt ttatattata tttttcatta
    64981 aatttatact atcttaccta atgaacataa ggtcttacgc agtagctgaa tccatatcag
    65041 cattgtctat aagtactata tatagattct tattatttat ttcatcatcc ttatttagtt
    65101 cattttcatc ttttaaatca gcatttttat atattttatt ttcacctaaa tcactcaata
    65161 attttgctga ttgtgctttt gttaatgtag ctccaccatc tttatcacct atttgaagta
    65221 ctagtttctt atcaaccaaa agcaattgat attttcctcc tatatctgct ttatcaggaa
    65281 gtgtatccat atatgttttt aattcactta aatcactagc agatgttgta gctggcatct
    65341 tattagtatc tgaataataa gacaatgctg cactcttaac tgaactataa tcagactcaa
    65401 cacttgctac cttagccttg tttatattac taaataaagc tggaactgcc actactgcta
    65461 atatacctat aattgcaatt actaccaata attccactaa agtgaaacct tttttattct
    65521 tctttaactt catttttatt cccccttaaa ttttttaatt aatacttact tataatatat
    65581 ctggttaaaa atctaagtaa aattatttaa acgtactaac caaatttatt tctaccatat
    65641 aaccattttt taaataaaaa aagctatgaa aataatagta tttataaata aatacaattt
    65701 catagccggt tgcaccacta actcaatatg ctccaaggtg cccacattac agaacataaa
    65761 tcaacgtttc tattttatta aattcaaatt atataactca taatgtatat acatatccac
    65821 ttatattata taatatctac catattttag caacactgtt tatactattt atatatacca
    65881 actttttaca tattgaaaca gaatttctag aaaaattata ctctacaaaa aaagaaacat
    65941 ctccctacaa ttattatata tcgtaaggag atatttataa attatcattt agttttatca
    66001 ttgtcatttc catcctttgc taatatttat ttgaaagcta aaagctatta caatatagta
    66061 ttttataaaa tcatctatta taaacattct atctattata gttatgattc agccacaatc
    66121 attatataaa cgaaactctt catttacaga tatgcttatt gtgcaaaatt actctttaca
    66181 gtaatcttat ataatccaga accctctcac aaacactaaa gatagaatag atactatttt
    66241 caattcttat aaataaactt tcaatagatt tttatattca ctttagattg tgactgaatc
    66301 atttaattat taggggttca tcttctaacc ttcccattgg aaacacctct ttctttattt
    66361 atatttacat aatactacat aatctgaatt ttttcaatac ttttttgtaa tattttttat
    66421 attcataata ttttgaataa tatgcccttt attaagttta ttttacttaa atatacataa
    66481 ttttaaacta taatctcttt ttacaataat atataccttt ctaacaagaa aaaaataaca
    66541 ttccataatt ataaaatcaa aaaagagttg ctcaaaaggt ttatatcctt ttaaacaact
    66601 ctcataaata caataaatta gaataattta cttactgatt cattagagaa tatcattctt
    66661 atagcatctc caaataaagg agctactgtt tttatcttta tcttatctat tcttttctct
    66721 tcaggaagtt gtatagtatc aagaactatt aactcactta tttctgaatt agcaattctt
    66781 tcaatagctg gacctgataa cacaccatga gtacaacaag cataaacatc ttttgctcca
    66841 aactctttaa gtgcatttgc agcattaact attgttcctg ctgtatctat catatcgtct
    66901 aatagtataa catttttacc tttaacatca cctattaagt tcataacttc gcatacatta
    66961 gctttcggtc ttcttttatc tattatagct attggaactt ctccatttaa agtattagca
    67021 aattttcttg atctagtaac acttcctaag tcaggagata ctactactaa atcttctatt
    67081 ttcttttcat taaaataatt tgctaatatt gttccaccta gtaagtggtc taatggtata
    67141 tcaaaatatc cttgtatttg agctgcatgt aaatccattg ttaatactct atctgctcca
    67201 gcagcagtta ttaaatttgc aactaattta gcagtgattg gatctcttgc cttagccttt
    67261 ctgtcttgtc ttgcatatcc atagtaagga ataactgctg ttattctacc tgctgatgct
    67321 ctctttaacg catcaattaa gattaataac tccattaagt tatcatttac tgggctgtta
    67381 gtagattgca ctacaaacac atcacaacct cttactgtct cattcatgtt tacacatatt
    67441 tcaccatcac taaatgtacc tacttcacaa tctagtactg atacacctat atagtcagct
    67501 attttttgtg ctaactcttt cgatgaatta cctgcaataa ttttaatctc gcttccgcta
    67561 gtattcattt atgtaaccct cctgaaaatt ttaaaatact aatgaatata atttataata
    67621 atttcgagct aaatttttta gccaaaataa taaactacat aattacttac tttggtcgtc
    67681 tttttgattc tttttttcta cccatccttc tttgataact tgtctttctc ttgctattgc
    67741 caaagcacca tctgggacat catgtgttat agttgaacct gttgctatat atcctttttc
    67801 ttctacaact actggtgcaa ctaaattaga atttgaacct ataaacgcat tgtcttttac
    67861 tattgattta aatttatttt taccatcata gtttacaaat actactccac atccaatatt
    67921 tacattttta ccgacatgtg catctcctat ataagaaagg tgagatgctt ttgagccatc
    67981 ttctataata gcatttttta cttctacaaa atcgcctatt tttacattat ttcctaagtc
    68041 actctttggt cttaaatatg catatggccc aacagtagaa ttttctccaa ccttactatc
    68101 aattatagtt gaatttttta tttctgtacc atcacctatt tctgaatttg ttatagatga
    68161 attcattcca attatacaat ctgaacctat ccttgttttt ccttgtaaca taacacctgg
    68221 atatattata gtatcattgc ctatcataac atcagattct atataggtag agttagtgtc
    68281 tattatagtt actccattta ccatatgaga ttcatttatt cttcgtctca taatttcttc
    68341 tgcctttgat aattctactc ttgagttaac gcccataagt tcttcaatag ttgaacctgc
    68401 aaatgcacca acttttaatc ctttatctct cattatttta gcagtatcag ttaaataata
    68461 ttcaccttgt gaattgttat tatttagcaa atctagtgct tctctaagac tctttccatt
    68521 aaaacaataa attccagagt ttatctcact tatcatcttt tcttctgaat ttgcatcttt
    68581 ttgttccact attttaagaa ggtcaccctt cttatctctt attattctac cataacctgt
    68641 tggatttcca actcttgttg taagaactgt agcatgatat ttattttcta tatgatattc
    68701 aaatagtctc tttagtgtct cttcttttat aagtggagta tctccacaaa gtacaactat
    68761 tgtatcttca tcattaatat attctttagc catctttaca gcatgtcctg tcccaagttg
    68821 ttctgtttgc atggctatta taatttcttc tgcaagcttt tctttaacta cctcagcttc
    68881 atgtcctaaa atcacgacag tatcttttac ccctgatttt tttgaaacat ctataatatg
    68941 gttaaccatt tcttttccac atactttatg tataactttt gggtatttag acttcattct
    69001 tgttcctttg ccagcagcaa ggattatagc tttaaagttc attgatacac tccattcaat
    69061 ctaatttaat tttattcgta ttattctctt aatcaatata cattataata catttatcta
    69121 acaatttgta tacaacatat acattatcac taataatata gcactttttt actaaaaagt
    69181 acatatttta atagaaattc tacatttaaa aagagcctaa atgttcgggc tctctccatt
    69241 ttatatattt attattattc agcagctact tcccattgaa ctaatgcttc atgatacgct
    69301 tgtaaaactg catcttctaa aacttgtctc atttctgcat ttattggatg agctatatct
    69361 ctgaaatttc cttcgcctac ttttctgcta ggcattgcta taaatagccc attatggcct
    69421 tctataactt ttatatcatg cactacaaac aaattatcga aagttattga aactatacat
    69481 ttcatcttac cttcttctgt taattttctt actcttacgt cagttatctt cataccaaaa
    69541 tatccccctt tgattttgac agaaattaat ttatattaaa attattatcg ctggaataaa
    69601 ttaatataaa ctgtttttta aataattcta tgaacaacta gggatatcct ctttttattt
    69661 ttacatattt ttctaaatat tttttttatt aatcttcatc tatctcaaat tcaaattctg
    69721 aatcttcttc ttcgtctaaa tcttcatttc tatattcatc tttaaaagtc tttaagtttg
    69781 gttctactac tattttatct ccattaacat ccaattgaat caaagaaata taatctttta
    69841 ccattttttc tgaaggattc gttgtagata tgaatactcc agtacctata acctctgcac
    69901 caaattcatt cataaggtct accattcctt ttatagtacc tccacccctc atgaaatcat
    69961 ctataattat aactttacta tctggtttaa gagcttttct tggcaaactc atactttcaa
    70021 ctttagaact gtttccactc acatatgtca tgctaagtgt aggaccttct gataccttta
    70081 tatcttttct tattataact agaggtaaat tcatagcttt tgcagtcata agtgccatag
    70141 gtataccttt tgtttccatt gtcacaacat aatctgcatc tacatactca atatttgatg
    70201 cgaatatctt tcctatctta gcagctattg tagggtcgta aattaggtca atcaagtata
    70261 aaaaaccacc agatagtatt ctagacttgt cacatattct ctcgcataat tccattaaga
    70321 actctgcatt ttctgctata gaagttttag gtatatattt tactcctcct gctgctcctg
    70381 aaatagttat aactttacct aaatgtagct tctcaaatac attttttaca actaataaat
    70441 cttcacttat tgttgatttt gctgcattaa attgatttgt aaaataactt aaagtatata
    70501 ttttatttgg attatcagac agtattttaa ctattgctcc aattctctct gttcttttaa
    70561 atttcattat tatttcctcc tacaagtacc tttatataac gttaaactaa ctaatgcata
    70621 acaattctta ttgtggtata atattactta tgttttatta tattcaaaaa taaaatacat
    70681 ataatattat atgcatatta attataaaat attgaacatt atattattat accacagaaa
    70741 tagttcatat tgtaggactt aattaaaaag aaaggtggtt ttattatgaa tatacacttt
    70801 ataggaattg gcggtatcag catgagtgca ttagctgaaa tatgtatcaa taaaggttat
    70861 caagtttcag gttcggattc aaacgaatca tacctattag acaaacttag ggaccaaggg
    70921 gcaactattt atattggtca gaaaaaagat aatatatcag atgatgtaaa tatggttgtt
    70981 tatacagcag cagttcatcc agacaatgaa gaacttgtgg ctgctaaaga aaaaaataaa
    71041 ttggtaatga acagagcgac ttttttaggc caaatcatga gggaatataa aaattctata
    71101 gcagtttctg gaacacatgg aaaaacttct acaacatcta tgttatctac aatctttgaa
    71161 tatgcagact tagacccaac tatactagtt ggtggtaact taagtatgat aggtggtaat
    71221 gttaagatag gtaattccaa ccactttata actgaagcat gtgaatatgt agatagtttc
    71281 cttaatttta acccaaagat atctatagtt ttaaatgttg aagaagacca tcttgattat
    71341 ttctctggaa tagatgaaat caaggcttct tttaacaaat ttggaaaatt attaccccca
    71401 gaaggctatt tcataataaa tggcgatgat gaaaatattg atgacatatt gtatgatgtt
    71461 aaagctacta ttataaaata cggcagagat tctgacaatg atgctgtaat aaaagatatt
    71521 cactttgaca atagtggtca tggaatattt agaatagaat atgaaggaag agatttagga
    71581 gaatttgaac tttctgtata tggtcttcat aacatataca atgcttcttc tgccataatg
    71641 gcagctcttg tttctggtat tgacttagag actataagaa aaaatataaa aatttataaa
    71701 ggtgtcggaa gaagatttga aactaaaggg tactataaga atgctttagt agtagatgat
    71761 tatgcacatc atccaactga gttaaaagca actttagctg ctgccaaaaa attaaaaaaa
    71821 tcaactttat ggtgtatctt ccagccacat acttatacta gaacaaaatc tcttttaggt
    71881 gaattttcag aggcattcta tgctgctgat aaagttatca taacagatat atatgctgct
    71941 agggaaaaag accctggaga tatacattct aaggacttgg ttgagaaatt atatcaaaac
    72001 aatgtagatg ccatctatat aaaagaattt gaagatatag taaaatactt acgtgaaaat
    72061 gttaaggata atgatttagt aataactgct ggtgctggtc caatttataa ggttgcagac
    72121 ttgttggttg aaaaataata tataaaataa tttaggctat caaatatcta atttgatagc
    72181 cttcttaaag tcttatttct ttccaatttc aaatatctca tttttaaggt aatcaaataa
    72241 acttgtatga acctcatagt aagatacatt tttcttagaa gtaactttta cataatcttt
    72301 tcccattgta tcaactttta catcaaaatt atcacctttt atatttatag taaaattata
    72361 gtcacttctt tccttacttt tctcaggttg aacttttcct acaaccctct ttgaaatcat
    72421 cttatgtgct aaatcatcaa atttccatct atgaactttt ttaatttctt ttccatgagt
    72481 tatagtaacc tctttccaat ctttataatg acttaaaaga tcaaacgaag tatagatact
    72541 ctcatctaaa agtttcttaa aagcatcctt agaactaact ggtactttat aatattcttc
    72601 tttatttaca gtatatactc taaaaaagtt aaggtcagta gaaaatttaa ctccatcatc
    72661 agaataacct tcataaactg cttcgtaaga agaagtagta tttcttactt tcttaaaatc
    72721 tgagaaaaat aactttattt catcccacat atcttcttca atttttatgt tttctatatt
    72781 tttatctgat atataaacct tcttcttctc ttttatttga gtccttaaag acctattatc
    72841 ttcttcttct ttttctacct tctttggcac ttcactttta tccttttcca tatttattcc
    72901 tattctaaag gatataaagt atattataaa gaaaacaact actcctatta atattgcagc
    72961 ttttttcttt agttttcttt tcttgctcat ttaaccaccc ctagagttta taatcataaa
    73021 acaattatat aactttttac actttttttt aagttttaat taattattac acttatttcc
    73081 ttggcaatta gtacatggtt caacgtgaac tgttatgcct tttacaaatt ccatatgttt
    73141 tattatatgt ttttcaagct tggtagcaat atcatgacct tgcttaactg tcaaattttc
    73201 atccacacat atagtcaaat ctacatatgc catagctcca tgttttctag tctttatatc
    73261 accaagattt ttaacacctt ctgtatctgc tacaataaat tttaattctt ttatctcttc
    73321 ttcatcaact gaaacatcca tcaattcatt aacagaactt ataagtatac taattcctac
    73381 tttagctaca aatatagcca ctacaactga cgcaattggg tctaatggtt taaaccctaa
    73441 aatagaacca cctataccta taaaagcagc tactgaagat aaagcatctg acctatgatg
    73501 ccaagcatca gcttttaggg ctggtgaatt tatcttttta gcaactttta ttgttattct
    73561 atattgatac tcttttatca ggattgaaat aactgatact actaatggta atattgatgg
    73621 aacacttaat gcatctaagt taaataaaga tttaattgcc tctataccta ttgttataga
    73681 aacgaaaata agcaatattg ataacacaaa tgatactaat gtctctgctt tctcatgacc
    73741 ataattatgc tctctatctc caggtctaga agatacataa ttgcctatta gtactcctat
    73801 agaacttata atatctgatg ctgaatgtaa tccatccgca atcatagcgc tagaattacc
    73861 aataacacca gctataacct taattattgt taatactaca ttccaaagta ttgattgtat
    73921 tgttatttta tttgcttctt cataccttgt ttccatattt attcctccct tatgaaacaa
    73981 tctaaatatt attaagaacc tacatttgaa cataaattaa acactctctt atatgcaaat
    74041 gtatactctt tgctttcaat agagattatc aatcttctat aaaatgaaaa atcacctagc
    74101 ttaagctagg cgatatatat attatacact attatttaaa tttatgcttc ctttattatc
    74161 tccactacca ttttagcaga atcatataag tcttttatag ctatatgttc acttagtgta
    74221 tgagcatttt tcataccaat tcctaaagtt atagcttcga taccattctt acttaatata
    74281 tttgtatcac tacctcctcc tgtagattca gtatgacctt ctatacctaa gtttgaaaaa
    74341 gccttctttg caagttttat tatttcagaa tcctcactaa catctattgg tgtatatgct
    74401 ctattgacat ctatctcaat ttgagctcca aaatcttttg cagctttttc aaaagtctct
    74461 accatgtgtt tagtttgaac atctaattta ttttctttta gacttctggc ttctgcaatt
    74521 atattaactt ctcctgttac tatatttgta gctgttccac cgtttataat tcctatgtta
    74581 gcagttgttt cttcgtctat tctaagtaaa ttcatatttt ctattgctct ggcagctacc
    74641 attatagcac ttattccagc ttcaggttca agacctgcat gagcagtctt tcctaatatc
    74701 ttaacattta ttacatcttg tgcaggagct tttactatta tttcacctgg agaaccacca
    74761 ctatctaata taaatgcata ttcagaatct atttttccat agtctaaatt tttagctcca
    74821 actaatccac attcctcaca tatagagaat acaacttgta tatcagtatg ttctatgtta
    74881 ttttcattta tatatcttaa gccttctaat atagctgcta tacctgcttt atcatcagaa
    74941 cctaatattg tagtaccatc actttttatt attccatttg cttcatccac tataggtttt
    75001 acccctatac ctggagttac tgtatccata tgtgaactaa ataatatttt ctttccttct
    75061 ttatttcctt ttaatgtagc tattatatta ccagtttctc catttgcttt ttcaccagca
    75121 ttgtctatta ctactgaaca acctatttct tctaattttt taactaatac tttagcaaca
    75181 ttcccctctt ttaaagataa actatctatc tgtactaatt caagaaactc atttaaaatt
    75241 ctttgctcgt ttatcataat tttcctccat aatataatag gtatttcaag atatattgta
    75301 gtgtaatatt taaatattat caatagtttt taaagttttg tactacattt cattaatttt
    75361 ttcagtaaaa taagaattga gtctaaattt tttcattttt attattttta cctaactata
    75421 tttctttgtc tagaatctat aaaacatagc caatatccta aactatttgt tctattatca
    75481 caagcttgat atcttaaaaa tcctgtgctt ccattgtctg gctgttcatt tctatttcct
    75541 gggatagcat aaatataatg ttttctctta ttatattcat catactgaac tccaaataaa
    75601 tagtgtctgt atttatatga atgtaaaact gcatcactat acatggtgta attcaacacg
    75661 tttaaatatc ctaaataagg cattgtatat ccacacaatg ttgtaggatt tatctctatt
    75721 ttccaccatc ttgtcttatc tatataatct gttacaaaag gttttacttc tttaaataat
    75781 ttcaaacctt tttttatttg tcttggcata agtaatgtac tggtttgttt atttccactc
    75841 tctccttgac ttactccatt agctcttttt attttttcaa tattattctg atctttttga
    75901 actttctttg atttagtagg ttttgaggaa acaaacctat cttcttctct atttacttca
    75961 aaaacttctt cttcctcttc ttcttcatca tattctatgt ctatatattc ttcctgtact
    76021 ttaaccctat tataattttc tatttcctta taattattga actcttctat ttcctcatca
    76081 tcagaatctt ctacttccgt tcttgctttt aactctcctt cttcaatatc ttcatcatct
    76141 tcccaatagt cttcatactc atcttcatca tattcttcta taatatattc ttcactatct
    76201 tcgtctctat cactttcctc attctcctct atctcttcgt cttcttcttc caactcttca
    76261 tattcttcat attcttcttc ctctacctct tgaaataata tgtcctcata attttcaagc
    76321 ttatttcctt taaatccaat taaaggtata tttctttcat acaataatgc aatacctttt
    76381 atctcatcat cataatcttc taaatctaaa acaaattcac ctcttccttg ttcatttaaa
    76441 attatatttc ctaagtctat taatccatgg tcatctttta ttgctactgc tttataccca
    76501 tctttaacat attttagatt ttctacatat aatgcaataa ttgctttttc atcatttact
    76561 tcaacttttg caaaagcttt tggcagtact cgttctttgt atctaaagtt tatgtcttta
    76621 gcttctagta ttatatagtc tcttttaaac tttcttttag aacccactgc aatcccccct
    76681 taacacagtt tctctaaaca atatatgtta acaaaattaa aaaaagtacc ttctatttag
    76741 aagatacttt tgtatttatt atatttgaaa gaactgaaaa ctcttctata ctaagagttt
    76801 caccccttct tttttcgtct atattagctt cttttaatac ttctcttatt tcatctttat
    76861 taagaaaacc aagacctccc aacgaattaa gaagagtttt tcttctttgt ccaaaggatg
    76921 cttttactgt tttaaaaaat atgtcttcat tatgaacatc atattttctt ttatctctta
    76981 catgtaaacc tataactgtg gaatctacat ttggttgtgg tataaacata tgtcttggag
    77041 cttttgccac aatctctgta tcacaatagt attgaacagc tatagataat gccccataat
    77101 ctttagtacc cggaatagca ttcattctat ctgcaacttc tttttgaacc atgactacta
    77161 tgtctgttac aggtatgtct tcctctaaaa atttcataac tattggagta gttatataat
    77221 atggaagatt tgctacaagc ttaactggcc caccatttaa tttatctttt actaattcct
    77281 gtatgtctac ttttagtata tcttgattta caacctctgt attatctaaa tctgacaagg
    77341 tatctttcaa tataggaatt agatttctat cgatttctat agccactact ttttctgcta
    77401 ttttacccat ctcacgagta agagttccta taccaggtcc tacttctatg atattatctc
    77461 ctcttgtgat tctagctcca gataagattt tatcaattat attactatct atcaagaaat
    77521 tttgacctaa agactttgaa aatttaaaat tatatttttg tactacttcc tttgttgcat
    77581 tatgtgaaga tagtctatcc attatttact cctttttaac ttattttgat atttaatttt
    77641 attaattata cttatttata tgtatttaca ttgatttaaa ataaggtgta tattataaat
    77701 aattcatctt aaattaaccg taattccaca atttatttta tatacatatt tttcatacat
    77761 taaacattac gtttagattt gttttaatgc atctataaat tcttctcttt caactccata
    77821 attattcaat ctatttaaaa attgttttgc atttccatat ccaattccaa gtatttttcc
    77881 aagtgcatct cttctaagtg atgcttcttc attacctata agaccatttc taattaagtc
    77941 aacttgctta aattcatctc ttttttctgt attttcagtt ctaactttat tcagagctgc
    78001 tattatactt tgaggagtag cattctcaat acctatatct ccatcctttt tagcttcctc
    78061 tctcggtaga aatgcatgtt tacatcctgg aacttctgat gctatttttt ttcttatttt
    78121 ttcacctgca aaatctgggt ctgtaaatat tataacacca cgtctttcat tagcagcttt
    78181 tatacgctcc attacacctt ttggaaatcc aaatccacct gtagttataa gttctgcatc
    78241 tattgctctc ttaactgctg taacgtcatc tctaccctct actactatta tttcttttat
    78301 catataaatt caccttttat tcgtttttag tttttcttta catctcttta ttttaaggtt
    78361 tttatttaat ttaatcaagg tactaaacta tttattttta tatagaaata tgcattttaa
    78421 ataaatatat caaaagagcc tagatataaa acttatattc taggcgcttt ttgtactaaa
    78481 ttttattgca aggcttagct ctctttttct gtctttccat catatttttc tatccattta
    78541 tttagtatag cctctctatt tcttgctgct gattttaaat cattatctat catctgttct
    78601 aatggattct ttggaaatcc ttctggcacc ggatttcccg tgtcaattgt agtaactgca
    78661 aaattcttag aatactcatt cattgcatct ttacttatag cccaatctaa aaatactttt
    78721 gatgcttctt ttatattatc ttttttcaca agtgcatttg attctatctc ccatccagaa
    78781 ccttcttttg gaaatactac ttctactgga taaccttcac cttttaactt tatacctcta
    78841 tttccaaaag atataccaat gggatattct cctgaagctg ctaatatagc tggagctgaa
    78901 ccagactgag tgtatacacc aatattttta tgcaatttat ccatatactg ccatgctttt
    78961 tcttcaccca taatttgtat aagttctgat actgttaaat atcctgttcc agaagaagct
    79021 ggattaggca tagatataag acctttatat tctggcttta ttaaatcttc ataagaacta
    79081 ggaactggta tgttcttatc acttaattct ttagtattta ctgttatacc agtcatccaa
    79141 gcatctattc caacccaaga aggaactttc ttatcatctt taaatacagg atttacctta
    79201 tcaaagcctt tcggagcgta tggctctagc atttctttgt catctaaaat tatcaaactt
    79261 gtagctgcaa ccccccataa cacatctgct ttaggattat ctttttctgc taataatctt
    79321 gctgttatat ctcctactga taatctaagt acattcaatt taatatctgg atatttctct
    79381 ttaaatgtag ttaaatatgt atctatacta tcttcttcaa ttgctgtata aactgttaac
    79441 tctcctttta tcttattatt agactttaaa tttgaacctg atgaagcatt attacatcca
    79501 gttattacac tcaataaaag tgttgataat attcctaaag agactaattt cctaaatttc
    79561 atttttacct cctctatgca acttcttttt gttgccattt ttgtgtctta gtttttaatt
    79621 tctttgttgt caattcatac aaaagtttta ctattatatt tgttaaaaac actaatactg
    79681 ccatagcagc tgatggccct atatacccta tctcttctaa ctttagtatc gatattgatg
    79741 cagtttgagt ttgaggtata tataaaaata ttaatgctga aacagtaacc attgaattta
    79801 cgaagtaata tacagccact tcaagtattg atgttatgga cattggaagt gttacataaa
    79861 aaaacacctt ataaaatgga atatttatag attttgcaac taattcatac tctctatcca
    79921 acatttttaa agatgaattt gaagttataa agcaaactga ataaaaatga actatattgg
    79981 ctataaccat tattgttgta cttccatata aaaaatttaa tggattactt gctgaattga
    80041 aaaaagttat atacgatata cctaaagcca aaccaggtaa tgccattggt atcattgata
    80101 aaaaataaga tatttgtctc agtctaggaa acttatccac tttttctatc atataagcac
    80161 taaaaaatac aaatatagtt ccaaatattg ctgttaaaat tgatattttt aaactgctta
    80221 aatatgtttt gattccattt cctgatatac ctttaaacgt atagtgttcc attgtaaagc
    80281 ttaaatcata tggccataat tttataactg agcctataac tgaaacaaat atcaaaataa
    80341 agattaataa agttataaaa ctacatgcta ttaaaaatac tctatctcta aacttatttt
    80401 ccttaatctt atatggcata gactttgcag ttatagtcat cccttgcttt ttacttgcta
    80461 tcttatctac tgtaaatgct attagtacag gaattaacat taccatagag acagtagctc
    80521 ccattggtat gttaaattga cctaccactt gtttatatat gtcagtagcc aatacattgt
    80581 agtttcctcc aactatcttt gctgctccaa aatctgtaaa tgcaagtata aaagatacaa
    80641 agaacgaact aactaatgaa tactttatac caggaattgt tattttgaag aacttcttaa
    80701 attcactagc ccctaagcta tctgctgctt cataaagtct ataatcagcc atcgaaagtg
    80761 ctacagataa tattaaaaag gcctgtggaa atgtaaacag tatttgacaa ataattattc
    80821 ctaatggacc atatagtggt acttctatgc ctataattgt aaagattccc ttgtttccaa
    80881 aaaggtatat aagtgaaata ccatatagca tagctggtgc gaaaagcgga aatatgccca
    80941 tagttttaaa aaagttttta aaccttatat tcgttcttgt tattgcatat gaatatataa
    81001 acgctaaaca tagtgatatt aaggatgaaa ttgtagatac aaaaattgag tttttaaatg
    81061 atgctataag agtgggattt tcaatatatt tactaaagtt tgaaattcct acaaaacttc
    81121 cacttgtatc cataaatgca ttttttaata gtgatattaa tggaaataat ataaatacta
    81181 ctaatgcaat cactaaaaaa actaaaaaac atttttgtat cttactatca cttattcttc
    81241 ttttattttt tattgttatc atttatccac ctctagctat taagcacatc cctctgattt
    81301 atattgtatg gttctttctt taggtatctt gaagtttatt ttagaaccta cacatagttt
    81361 catttgttcc actacctttg aaactacgtc caactttatt tgcttagaac aatattcaac
    81421 tgtaattcta tagttgaatc ctctaaattc tatatccaaa atctctccta cttttacatc
    81481 aatttcttta aagtctctat taaattctac ctttatatct tctggtctaa ttgctatcat
    81541 attcacatca tcatctataa aatttattgt tcctataaaa tcagctacaa aaggtgtttt
    81601 tggactttta tagacttctt taggagttcc aatctgtatt acctctccat tattcataac
    81661 aaccatttta tcagctaaac aaagagcttc ctcttggtca tgtgttacca ttatagttgt
    81721 gatgcctagt ttgctatgta aacttttaat ctgttcccta agtttaagtc ttacttttgc
    81781 atctaatgca gataaaggtt catcaagaag caaaaagtct ggctctaaag caatcgcacg
    81841 agctagtgca accctttgtt gttgtccacc tgataattgt gatggatatt ttttatcata
    81901 tccacttaat tctacaactt ctaaaatatc tctaaccttc ttatctatat cagatttact
    81961 tatattttta ttttctaatc caaaggctat atttttatat acattcatat ttggaaatag
    82021 tgcatatgat tggaaaacaa taccaaagtt tctactttca ggtggtgagt tagtcatatc
    82081 cttatcattt attattattt ttccatcatg tttatcctca agtcctgcaa ttattctaag
    82141 caatgtactt ttaccacaac cacttggacc taataagcaa ataaactccc ctttttcaat
    82201 atcaatagat atgtctttta gcaccttaac ttgtccataa cttttaccta cattttttac
    82261 ttgtaaaaaa gacatttata attcctcctc ctaaagctga cttaaactta aataattctt
    82321 ctctagttaa atattctcac ttccaatttc aagttatcaa gaaacaattt accttttatt
    82381 ttttctcttt tttcatgata tataacacaa gttcccaaat taaaatttat acatatctta
    82441 ttaataatcg ctcaataatt tatttacttt atatataata tctatacttt aataatgaaa
    82501 ttaaaatata ctattttaaa ctgtagtata tctatttcta aattaatctc taaaaataaa
    82561 aaatatacca ttcaaactga atggtatact tactctatac atttttatag aatattcact
    82621 tttcttgacc tatattattc tcaacttata ttattataat aaaatctact taaaaaaaaa
    82681 ttttcttttt actccatttg tatagtttta cttaactctc tttttctgtc ttaccatcat
    82741 attttgatat ccatttattt agtatatctt ctctattttt tgctgctgat tttaaatcat
    82801 tatctatcat ttgctctaat ggctttttag ggaatccttc tggtattgga tttcctgtag
    82861 aaatagtagt aactgcatag ttcttagagt attcgttcat tgcatcttta cttatagccc
    82921 aatctaaaaa tacttttgat gcttctttta tattatcttt tttcacaaga gcatttgctt
    82981 ctatatccca tccagaacct tcttttggaa atactacttc tactggatag ccttcctctt
    83041 ttaactttat acctctatat ccaaaagata taccaatagg atattctcct gaagctgctg
    83101 atgtagctgg agctgaacca gattgagtgt atacaccaat attctcatgt aacttatcca
    83161 tatactgcca tgctttttct tcccccataa tttgtataag ggctgatact gttaagtacc
    83221 ctgtaccaga agaagatgga ttaggcatag atataagacc tttatattct ggctttatta
    83281 aatcttcgta agaactagga actgggatgt tcttatcact taattcttta gtgtttactg
    83341 ttatacctgt catccaagca tctatcccaa cccaagaagg aacttcttta tcatctttaa
    83401 atgcagactc tatttcttca gaaccttttg gagcatatgg ttctaacatc ccctggtcat
    83461 ctgcaaccaa taagcttgtg gctgcaactc cccatacaac atctgcttga ggattatcct
    83521 tttctgctaa taatcttgct gttatatctc ctgttgatgc cctaacaata tttaatttaa
    83581 tatctggata tttctcttta aacgtagcta aatatggctc tatactatct tcttcaattg
    83641 ctgtgtaaac tgttaaatcc ccttttgtct tactatcaga ctttgaatct gaacctgatg
    83701 aatcagtact acatcctgtt attacactta ataaaagtgt tgataatact cctaaagaaa
    83761 ctagcttctt aaatttcatt tttacctccc ctatgcaaca tctttttgct gccatttttg
    83821 tgtgttattt tttaatttct ttgttacaaa ttcgtacaaa agtcttacta ttatatttgt
    83881 taaaagcact aatactgcca tagcagctga tggtcctata tatcctatct catctaactt
    83941 tagtattgat attgatgcag tctgagtttg aggtgtatat aaaaatatta atgctgaaac
    84001 agtaaccatt gaatttacaa aataatatac tactatttca agtattgatg ttatagacat
    84061 tggaactgtt atattaaaaa atactttata gaatggaata tttatggatt ttgcaactaa
    84121 ttcatactct ttatccaaca tttttaaaga tgaatttgag gttataaaac aaactgaata
    84181 aaaatgaact acatttacta taactaatat tgctgtactt ccatataaaa aatttaatgg
    84241 attacttgct gagttaaaaa aagttatata tgatatacct aaaactaagc caggtaaggc
    84301 cattggtatc attgataaaa ggtaagctgt ttgtcttaat ttaggaaact tatccacctt
    84361 ttctatcata taagcactaa aaaatacaaa tatagttcca aatattgctg ttaaagttga
    84421 tattttcaaa ctgcttaaat atgtttttat tccacttccc aaaatacctt taaatgtata
    84481 gtgttccatt gtaaagctta aattatatgg ccataacttt ataactgaac ctataactga
    84541 aacgaatatt aaaataaaaa ttagcaatgt tataaaacta catactatta aaaatactct
    84601 atctctaaac ttattttcct taatcttata tggcatagac tttgcagtta tggtcatacc
    84661 ttgtttctta cttgccatct tatctaatgt aaatgctatt agtacaggaa ttaacattac
    84721 catagaaaca gtagctccca ttggtatgtt aaattgtcca actacttgtt tatatatgtc
    84781 agtagccagt acattgtagt ttcctccgac taccttagct gctccaaaat ctgtaaatgc
    84841 aagtataaag gatacaaaga atgaactaag taatgagtac tttataccag gaattgttat
    84901 tttgcaaaat ttcttaaatt cactagcacc caagctatct gctgcttcat aaagtctgta
    84961 atcagccatc gaaagtgcta cagataatat taaaaaagct tgtggaaatg taaatagaat
    85021 ctgagaaata attattccta atggaccata tagtggtact tccagaccca tggttgtaaa
    85081 gattccctta tttccaaaaa ggtatataag tgaaatacca tataacatag ttggtgcaaa
    85141 aagtggaaac attcccatag ttttaaaaaa atctttaaac tttatgtttg ttcttgttat
    85201 tgcgtatgaa tatataaatg ctaaacatag tgatattaaa gatgagactg tagatacaaa
    85261 aattgaattt ttaaatgatg ctataagact tggattttca acatatttac taaagtttga
    85321 aattcctata aaatttccat ttgtatccat aaatgcattt tttaataatg atactaatgg
    85381 aaataatata aatactatta gtacaattac cgaaaaaatc aagaaatatt tttgtattgt
    85441 gctatcactt attcttcttt tattttttga tgttatcatt tattcacccc tagctattaa
    85501 gcacatccct ctgatttata ttgtacaatt ccttctttag gtatcttaaa gtttatttta
    85561 gaacctatgc acagtttcat ttgttctgca actttggaaa ctacatctaa tttcatttgc
    85621 ttagaacgat actcaactgt aattctatag ttaaatcctc taaattcgat atctaaaatt
    85681 tctcccactt ttatatcctt atctttagaa tctctattag actctacttt tatatcttct
    85741 ggtctaatag ctatcttatt aataccatca tctataaaat ttattgttcc tataaaatca
    85801 gctacaaaag gtgtttctgg atttttatat acctcctttg gcgttccaac ttgtattatt
    85861 tctcctctat tcataacaac catcttatca gctaaacaaa gagcttcttc ttggtcatgc
    85921 gttaccatta tagttgtaat gcctagtttt ctatgtaaac ttctaatctg ctccctaagt
    85981 ttaagtctta cttttgcatc taatgcagat aaaggttcat caagaagtaa aaagtctggc
    86041 tctaaagcaa tcgcacgagc taatgcaacc ctttgttgtt gtccacctga taattgtgat
    86101 ggatattttt tctcatatcc acttaattct acaacttcta gaacttcttt aactttttta
    86161 tcaatattag atttacttat gtttttattt tctagtccaa aggctatgtt tttgtaaaca
    86221 ttcatatttg gaaataatgc atatgattga aaaactatac caaaatttct actttcaggt
    86281 ggtgaattag tcatatcctt atcatttatt attatttttc caccatgttt atcctcaagc
    86341 cctgcaatta ttctaagtag tgtactcttt ccacaaccac ttggacctag taaacaaata
    86401 aactctcctt tttcaatatc aatagatatg tcttttaaca ccttaacttg tccataactt
    86461 ttacctacat tttttacttg taaaaaagac atttataata cccctcctaa agctaaccta
    86521 aacttaaata aatcttcttt atttaagctc ttgcttccaa gttcataatt atgttgagaa
    86581 taaaatccgt ggtaatctga acctcctgtg accactagat tatacttctt agcataagaa
    86641 agcgactctt tttctagttc tttgttgtga gatggatgat atacttctat tccatctaat
    86701 cctactttta ccatctcttc aatagcgcta aaattatcca tttgacccgg gtgagctaac
    86761 acacaaattc caccagaatt ttttatagct tttattgcat ctctgtaatc cacatatttt
    86821 aaagatacat ataccttgcc gtttcctcta tgaaacaatt ctttatatag atttgaatat
    86881 atattatctg tatatccttt atctataaga gctttcatta tatgttgttt aaatattcca
    86941 gtctttgatg aatatttctt tacatcttcc agagatattt tgtatcctaa atctataatt
    87001 ttatttacca tcttaacaga tgctttatgt ctgtcacata ccattggatt gcacaaattt
    87061 tttatttctt gactgtttaa atctatgtta tatccaagaa tatgaactct ttttctattt
    87121 ttataatcat aggctgaaat ttctatacct ggtattattg ttattccata atgctctcca
    87181 agcacaagag cttcatccaa tccagataat gtgtcatgat ttgttattgc taagatattt
    87241 atattctttt ctgctgcttt ttttattatt tcttcagtag taaaattatt atctgatata
    87301 cttgtatgac aatgtaactc tgttagcaat ctttatacct cccttttata aactgttttt
    87361 ccatttacaa tagtatttac aacctttgga tattcactat caatatctac taaaactaaa
    87421 tctgcttttt tattttcttt tatactacct ctttcatcat atataccaac tgctttagca
    87481 ggatttgagg taaataaact gactgcttta tttaaattat ttattctttc tgcaactata
    87541 cacatagact ttatcatagc tgatggaaga taatccgaac acacaatgtc acaacaatct
    87601 tccaatatag catccattgc ttttaaattg ttattatgag atttacctct tacaatattc
    87661 ggtgcaccta aacaagttag aacacccaat ttctttgaat gttttgcaac ttcaagagtt
    87721 ataggaaatt cagatacatt tatgcctata ttttttatga aatccatttt ttctaatgta
    87781 tcatcatcat gtgatgcaac atttatattt ttagttttgg ctatacctat aatattagct
    87841 attttattcc aatctaaatt atcatgcaga tttactaaat catctaacca tgtatctaca
    87901 taattttctg taactcccca cactttagta gcatactctt tataaaatgt tggatttgta
    87961 tactgacctt gtcctggaga atggtccatt attgataagt agtctatttt attttcatct
    88021 aatagttcta aaaccttttc taaaccttca taatacaaca cttcatatct taaatgaact
    88081 ttatgattta tgctcttact atctatattt ttatatgaat caatattttt aatcatcttt
    88141 aaagagttat caatacttct aactcctact ccatccccta aagatataga atgataaact
    88201 gtagttattc catttgcagg tagtttttta tctagttcat aaaatgccat attaaatggt
    88261 aataatgtac ttggtctcgg ctctatttcc ttttcaatgg catcactgtg aatatctata
    88321 ataccaggta ttacatataa gttttttgca tctattattt catctatctc attaaagtca
    88381 aatgcgtaat caatcttatc atttgatact ttttttattg tatctccctc tatgattaaa
    88441 tgaccatttt ctaaaatcat atcttctaat acaatatttg cattatgaat tagttttatc
    88501 atctaggcta ccccactttc acttatagtc ccttttgtca tagtaaaggt cttatctata
    88561 acagtttcca taaactctat atcatgaaaa attcctatca ttgtagttcc ttgactttta
    88621 agctccaaca ttttttcttt tacatatatt ttagattgat tatcaagtga tgcagttggt
    88681 tcatcaagta acaaaagtct aggcttttta accatagctt gagctaaatt taatcttaat
    88741 ttttctccac cagaaaaatt atttggatac gcatcccata aattttcctt tatttcaaat
    88801 tggttaagta tatcttttgc catatcatac gaacaatctt tttcaaaccc actctcaatt
    88861 aaagaatgta ctaccagttc aattgcagtt attcgtggaa gtgtctttaa aaactgtgat
    88921 acatatccta tttctttctt tctaagattt atcatttctc ggtctccaat tttagccagg
    88981 tctacattac catatatttc tgaattaaaa attatttctc cagttgttgg tatatatgtt
    89041 ttatagatac tttttaatat ggtagactta cctgctccag atttacctat tattcctaaa
    89101 aactcacctt ttgaaacttc aaaacttata ttactacatg ccgttatagt cttgttcaat
    89161 gcatgtaact caaagtcttt actaaaatta tttacttcaa gtatagtttc tttcatttta
    89221 ttaaaaaccc ccattttgct tatttacaaa cgatatttat agcaatgaat ttataagttg
    89281 ctgagtatat gggtgttgtg ggtcctccat tatttggtct gtaagaccag actcaattac
    89341 acttccattt ttcattacaa ttgtcctatc agcaagcatc tttataacat caaagtcatg
    89401 ggatacaact atcatagaaa tcttaagttc tctttggatt tctcttatta aatccaatac
    89461 ttccgcttgc acagaaacat ctaaacctgt tgtaacctca tctaatagca atattggagg
    89521 gttatttgaa agagcttttg ctatttgaac tcgttgttgc attcccccac taaagtattt
    89581 tggtttatgg tctattcttt ctacaggtat attagttttt attaaaagtt ctttgcctct
    89641 atctcttatt ttttcaacat tatataaatt tgccatcaat aatttttctg ctatattacc
    89701 accactacta aagtctaaat ttaaaccaag gtggggattt tgatacacaa tacccataaa
    89761 atgattcttt atgtatctct ttttttggtc tgaaagctct agtatatttg tgtctttatt
    89821 gtaatgggat atatatactt ctccactatt tgcatgttct tggaaaaaca gtatttttaa
    89881 taaagtgctt tttcctgaac cagattcacc tacaattcca aggacttctc cttcataaag
    89941 ttctaaattt acattattac atgcaactat agaaccacaa tacttacata tattattgct
    90001 ttcatatcct gtgctttcaa aacacttttc acacccatct ccataaataa gattgatatt
    90061 tcttcctatt aatacttgct tgtctatatt ttctaatctt ttagagtcat ttattttact
    90121 cattccaagt acctcctatt gtcacatcta tactgttatt ttttaatcta actttttcac
    90181 aataacttgt atctgagcaa ctatagtact tttcattagt gtcactgtca tatacttcat
    90241 ctaaaaatgt atttgtactg ttacataaac tacagtattt tccttcaaac ttttcctctt
    90301 caaacttata atcctcaaac tctaaaggtt ctacctttgt atatggagga atagcataaa
    90361 ttcttttttc tctacctgca ccaaacaaga ataaattgtc tgacatattt aattttttta
    90421 tatcaaatct aggtataggt gatggattca ttaaatatct atcatttact ttacatggat
    90481 attcaactgc tatagatata tcattaaatc taactatatc ttcataaaga tatacccaca
    90541 tcctactata atcttcttct gaatgcattc ttcttgtttc tacttcacta ggttctacta
    90601 ttctaagggt ttctggtatt ggtacttgaa atattagtat ttgattagat ttaagttttt
    90661 cttctggtat tctatgtctc gtttgaatca atgtagcttc taatgtatct tttgtggttt
    90721 ctacattact tacacttgca ataaagctcc ttatattaca agcatttgta gaatcatcac
    90781 ttccttggtc aattactttt actacatcat ttttaccaat taatgatagt gttaattgta
    90841 aacctccagt accccatccc ttagctatag gcaactctct tgaaccaaag ggaacttgat
    90901 aaccaggtat tgatattgct tttaatatct ttcttcttat ttcttttttt gtgttttcgt
    90961 ctaaaaaacc atagttatat atcacctaaa cttacctcct caaactattt tattctttct
    91021 acatccgctt taaaatctac atagtgagga agcttaaagt gagatataaa tcctaatgaa
    91081 tctattccat ctatatgaag caatacaaat tcttcatctt gtgctagttt ttcactattt
    91141 ggctccaaag atttgtctag tatggccata gatatagcct tattctcatt attccccata
    91201 caaagaccat atcctaaaga catcttattt ttatcttccg aattagtttt agaattttca
    91261 gtcttatttt gtgatgcttt tgaaactatt tcacattcag taaccagcat atcccctatt
    91321 attatttctt catcttcata gatgggatgt tttactttta taaatacatt accagttcta
    91381 agttcactta tatatggatg ttcatcacca aaacctctta caatagcata agcaaaacct
    91441 gacactactc cactttcacc tctagttaat gcctgtaact ttgctgacct agaaactgga
    91501 aatcttaaac tatttcttgt aatatcatca ataacttctt ccaatttgtt ttcacaaact
    91561 tcttcttgta ataaattttc ttttctaaat atattagata tttttgtaaa ttcagggatt
    91621 tcatctactg tttctaattc atttataaaa ctatctctaa aatgtctatt ctttttttca
    91681 tcttcttcta ataattctac attcaatatt ctattttcat agtctctagt tgccccaaga
    91741 tactgacctc ctggtatatc tttaaaagta gctgatattc ttctttttat tctcatttct
    91801 tctgtattta tgggttctga atatgtaatt ctaggcattg ttgaccttaa agctcttaca
    91861 tagaaagagg cttctaatat atcaccttga gcttgtttta tagccaatgc tgatagttcc
    91921 ttgttgtata aacttccttc agacattact ttgtctacag cataagacat ttgttcctca
    91981 atctgcttta catctatagg caagctatct ccttttaccc tataaaactc aagtatattt
    92041 ttagcctgta atatggcttc ttctccacct ttaactgcta cataagccat ttctataata
    92101 cctcccaact taattttgat gaccttggta taaatgcaat ttctcctttt ttatctacta
    92161 aaatcaaatc tattcctatt ggataatctt tatttatgct agataatctt tctataaatt
    92221 cttctctaat tccatttata tatatactat ttttagattt tataccagga ccactcaaat
    92281 caagctttat atacttagtt tgagagtacg ttgataaatc atttacttga tgtactatag
    92341 ttgcactttc atcaggatat tcaaaagaac ctattttaat ctgtaatata tccacttctg
    92401 aattaatatc taaaaatata taatctgatt ttctataatc tatttgattt gacattgtaa
    92461 gtgctcttat attttttata tctttttgag tataattttc tatatagaag gtaacttctt
    92521 ggtctaataa agtgattgct ataccaattg ctccattcaa tagttttgta ttattttcta
    92581 tatttatatt atctatatta tttaatttcc caggaaatga agcactatct agcatctttc
    92641 tatatatttc ttgtgtataa tttaccatga ttctcctcct atttaatcca tagcattgaa
    92701 ttgaactttc gttttattaa tcaagctaaa ttcatcaata atctcttgtt cataccttaa
    92761 gttctcttca tatacacttt tatttatgta attctttaac tcatcaaatt tactgttatt
    92821 gctatgtaaa actgcatcta taactgctaa ataaatggct ttcttattat tgttttctga
    92881 aactattccg taacccaagc tttcatctac ctttacacta cattcagtaa ctaatatctc
    92941 tcctaaatta aatgttgtgt tttttacact ttcattagct cttaccatta ctgtagccaa
    93001 gttaggttct ttaattatag aaatacttga tgtatctttt atcattgagt atatcttttc
    93061 tagtttctct aagttcatat ttatcaatat cttagtacta tcaactgtat acaaaattca
    93121 acctccttaa tctacctttc gtttacttct ctttaataat ataataaaca acttaagtta
    93181 agtgaatatt agagttgagt taattctaat gaatatcttg taaattcttt tttaacaatg
    93241 ttaagaaaat atattttaaa atatgaatta aataaataaa aacaaactat ctcaaaatat
    93301 gataaacttc tttttcattg ttgaaatgat gaaaatatat ttgatttcat aaaaaaaaga
    93361 gtgcctcatg aactaaaaaa ctcattttga ggcactctca tcctatactt ataatgactt
    93421 attatatata tcattataag atttaatttg tctattttat attaaaaaat ttctttgcat
    93481 tttcttttgt aactctacat acttcttcat aagatatccc tttttcctgg gctatcttat
    93541 cagcaacaaa agatacttgg gaaggattgt ttctctttcc tctatgtggc tctggtgaca
    93601 tatatggtga atcagtctct atgaataatc tttctagagg tatttctctt actacttctc
    93661 tagttttttt attattctta aaagtaaccg tgcctggaat tgatatataa caacccatct
    93721 taacatattc tctagctaac tctacattcc cactataaca atgaagtaca catcctattt
    93781 caggattttt agtattcttt attatctcaa aagtatctcc atgtgcatct ctatcatgta
    93841 ttattattgg aagttttagc tcattagcta attcaatttg cttcttaaac cactcttttt
    93901 gttcttctct tggagaataa tcataataat aatctaatcc aatctctcca atagctacaa
    93961 ctttttcatt ctcagttgca agttttctta gagtttctat tgctgtatca tctagttttg
    94021 atacatcatg tggatgtacc ccaactgctg aatatataaa gtcgtatttt ttcgcaagtt
    94081 caattgctgt tatcgacgtc tctatatctg ctcctggatt tacaaccaaa tctacacctt
    94141 tatcttttaa tgaaccaata agttcatctc tatcttcatc aaaactttca tcatttaaat
    94201 gtgcatgtga gtcaaataac atctttaatc tccttactat ttaattacct actaacgaac
    94261 ctcgcatcca tctttagccc cttgtgtaaa tggtaaaaca tatgggtctc catcatcacc
    94321 tgctgctaat atcatacctt gagattctac acctcttaat ttaactggtt ttaagttaca
    94381 taccactgta acctcttttc ctactaaatc ctctggctta tagtattcag ctattcctga
    94441 tactatttgt cttgtttctg gtccaatctt aacttgtgat actaataatt tatttgcttt
    94501 tggatgtttt tcacagctta ttattttccc tactctaagc tctattttgt ctaaatcatc
    94561 aatagtaatt tcttctttat gctctaaagg tttttcttct actggaggtt tcccagagaa
    94621 cattttagtt aattcttcta tctctttttc tacatctaat cttgggaata aaggttcacc
    94681 tttaaatact tttacatttt ctccaataag accaaatgtt tttgtacttt cccatgtagt
    94741 tatatcatct tgacctttta ttcctatatg ttcatatatt ttattagcag tctcattcat
    94801 tataggattt attagtgttg cgattattct tatagattca cataaattat ataaaactgt
    94861 gtctaattca tctttcttcg tttcatcttt agctagtgcc caaggcatag tttcatctat
    94921 atatttgtta gttcttctaa ctaatttcca tacactctct aatgcttcat ggaattgcat
    94981 tttatccatt tcagcttcaa agttttctct agttgaaaca gcttgttctt ttaagtcagc
    95041 atcaaaatta gttgaaactt ttgctgttgg tattatacca ttattgtatt tttcaaccat
    95101 agctacagtt ctacttatta agtttcctaa atcatttgct aaatcataat ttattctcgt
    95161 tacaaaattt ctatgagtat aacttccatc ttgcccaaat gcaaactctc ttaataagaa
    95221 gtacttaagt gtgtctattc catatctttc tattataggt tctggataaa ccacattacc
    95281 ctttgattta ctcattttat catcagcaaa tagtatccat ccatgtccaa atacttgttt
    95341 tggaacttct agacctaaag ccattaaaag cgctggccat attattgtat ggaatctcac
    95401 tatttcttta cctactattt gaacattggc aggccaatat tttttaaatt cttcatcatt
    95461 atcagtcata taacctaatg ctgttatata gttacataaa gcatctaccc acacataaat
    95521 tacatgtttt tcatcaaaag gaacttttat accccagtca aaagtcgttc tagttacgct
    95581 taaatcttct aatcctttgt ctaaaaagtt tgccaccatt tcattttttc tagcagcagg
    95641 aaaacagaaa ctatcatctt taaataactc ttttaatctg tcttcatatt tagataatct
    95701 aaagaaataa gattcttctt ttactaaata tgtttctctt ccacagtcag ggcatttatt
    95761 tccttctagt aattgagatt ctgtccagaa actctcacaa ggagtacagt atctaccttc
    95821 atattcacct ttatatatat caccttgctc atatagttta gtaaatatct tttgtattat
    95881 gtctgtatgt cttttttctg tagttcttat aaaatcatca taagatatat ccatagtatt
    95941 ccataaggcc tttatatctt ttatcattcc atcaagatac tctatctctg acattccttg
    96001 ttctatggct ttcttttgaa ttttttcacc atgctcatct gttcctgtta agaactttac
    96061 atcatatcca cagaaacgct tgaatctagc tattgtatct gcagctactg ttgaataagt
    96121 atgtcctatg tgtaatcctc cacttggata gtatattggt gttgttacat aaaaactcgg
    96181 tttgctcatg ttatttacct cctaattttt tatcgttcgt aatcatatga ttctttcatt
    96241 taaataataa aaaatcgcct cctatgagtt aacatagggg cgagattatt cacggtacca
    96301 ccctaattca tctataatag ttatgtttca catgaaatgt agaaacattt tatagatacc
    96361 ttagtagact ataacgtgtc caaccgtttt accctagcac acttgctcag ataaaaagct
    96421 ccaagaccat cttcaataaa gcttttgaca ctagctttca cctaacctag ctctctgata
    96481 gaaaaaacct ttatttactc ttcttttcat agcttgtaat ttgtattact atttgtttct
    96541 atataatcac tatattataa aatttttccc atattgtcaa caaattatac aaatattaca
    96601 tagcaaataa aaacagctgc tatgacacct gcaacatggc taatcatagc acatgataat
    96661 gtatgtctag tattttttat cttcagactt ccatagtata ttgccattgt gtaaaaaata
    96721 gtctcagaag aacctacaat cgtagcaccc attttttcta ctaaacttcc tattccaact
    96781 ctatttgcaa gttcactgta catccctaaa gcaccacttc cagataaagg ttttacactt
    96841 attagaccta ttaattcttt tggtatactc ataatatttg ctattggtgt aaatagccat
    96901 tctaacatat caagaccttt accagttttg aatattccta ttgccaaaaa tatacctatt
    96961 atatatggta ggatatccca tgctgctttt aatccatcaa ttgctccttt tacaaaacta
    97021 tcatacacat ctattttttt atatttacca taaactacta tgtacagaat tattattggt
    97081 ataagtaagt ttgaaaatat ctccatgtat ctacatcccc tttatctata tcttctttga
    97141 aaaaatttac atgttattat tgctactgct gtagatatta tggttgcaaa taaagtcgta
    97201 aaaattattt cacttggatt ttgagccccc atatccatcc ttaatttgat tatagtaaat
    97261 ggaattattt gtatagatga tatattgatt accataaaca taatcatatc attagtggct
    97321 gtatctttct tgttattcaa agtttgaagc tcactcattg cttttaaacc aaatgctgtt
    97381 gcaccatttc cagcacccaa catatttaaa gcaatattca taactatata agacattgct
    97441 ttatgacctt ttggaataga aggaaataat cttctcatta taggatttag tttttgacct
    97501 atcttttcaa ttaaacctga ttcttttgcg atattcatta tccccatcca gagtgccatg
    97561 ataccagcta gacttattgc aaactctata ccttttgatg tttcacttaa tataacttta
    97621 tttaattcac ccatattttt agaaatcaag cttcctatta ttcctatcga taccatataa
    97681 aaccatattt ttcctattat aatcatcctc ctctttaaat aattttatga tagttaggta
    97741 atactttata cttgaaagtt gtactatatt atgttttagt ataagtgtgg gagtttttac
    97801 aatttttaat atttaataat aattaaggag agaaataatg agaaatctaa cacttcttac
    97861 tgatttatat cagcttacta tgttaaatgg ctattttgaa aagaatattc atgaagacat
    97921 tgttgtattt gatatgtttt ttagaaaaaa cgcttgtgat ggagggtata ctatagtttg
    97981 tggtattgat caggttgttg aatacataga taatcttcat ttttcagatg aagatttaga
    98041 gtacttaaaa aacttaaatt tattttctga caagttctta aaatttttaa aagaatttaa
    98101 attcacagga gatatatatg ctgttgagga aggaactatc atgttcccta atgagccatt
    98161 aattactgtt aaggcacctt tatatcaagc tcaacttatt gaaacagcat tgttaacaat
    98221 agttaatttt cagtctctta ttgctactaa agcatctaga gtttgctttg cagctcaagg
    98281 agaccctgta tttgaatttg gattacgtcg tgcacaaggt ccagatgcag ggatatatgg
    98341 ggctagggct gccgtagttg gtggttgtgc tggaactgca aacgtactag cagggaaaat
    98401 gtttgatata cctataattg gtacacaagc tcacagttgg gtacaaaaat ttgataatga
    98461 acttgaagca ttccaagcat atgctgatgt ttatccagat aaatgcctac tacttgtaga
    98521 tacttatgat gtattaaaca gtggagttcc taatgcaata aaagtattca aaaatatttc
    98581 tgaaaaagga tacaaaccta tgggtataag acttgactct ggagatttag cttatttatc
    98641 taaagaagct aaaaaacaat tagataatgc tggcttttca gatatcagca tcactgcatc
    98701 taatgaccta gacgaatata ccataacatc acttaaagca gaaggagcta ctataaattc
    98761 atggggagta ggtacaaagc tcattacatc tttcgattca ccttctcttg gaggagttta
    98821 taaattagct gcatcttgtg aaaaaggagt tcttgaacct aagataaaga tttctgagaa
    98881 tccagaaaaa ataaataatc ctggttacaa gaaagttata agaatatata acgaagacaa
    98941 taaggcagag gcagatttga ttatgttaca tgatgaagtt atagatgagt ctaaaccttt
    99001 agaaatcttc catcctactt atacatggaa aacgaaggta tttacaaatt acaaagttaa
    99061 agagcttctt aaaccattgt atataaaagg cagatgcaaa tataataaaa aagctgttct
    99121 tgaaataaaa aatcatgtac aatatgaatt aagtactatc tgggaacaat acaaaagact
    99181 ttcaaaacca cacatttata aggttgattt atctagaaat ctttggtatt taaaaacaca
    99241 aatgatagat tctaaaaaag ttttatagca ttaattattt ttatttaagg gggactgttt
    99301 atttatggat gttacagatt acagaatcat agaaatttta caggatgatg ggagaatttc
    99361 tatgaaggac ttaggaaaaa tagttggttt aacttctcct gcagtttcag aaagagtcaa
    99421 aagattagaa gagtctggtg ttatagaagg atataaagct attgtcaacc cagattcatt
    99481 aggcagagtt ataaaggcat ttattcatat ttctcttcca agcaatggat atacagaatt
    99541 tattgagtca gctgcaaagg accctaggat tgtagaatgt caccatataa caggtgatga
    99601 ttgcttactt ttaaaagtaa ttgtaaaaga tatgtacgag ctagaaaatg tgattgatac
    99661 tataaagaaa attggttcta ctaagacctc tgttatacta tcaacgccaa tacaagcaaa
    99721 atcaatattg taatataaat taaatactaa tggtttatta attaatagac cattaagtat
    99781 ccttaataaa atttcaattt ttgatataaa aaaagttgtc tttcataatt ttacaacaaa
    99841 gacaacttct tttatgtttt tgaaatccgt atatttcaaa cttcaattaa tcatctaaaa
    99901 ttcaattata ttaactaaat ttatacatgt gtataattac tcttatacaa gtaatttgtt
    99961 aaaatatatt ccatatatgt ctttaacaca ctgacttctc cactcttgcc caataatttt
   100021 actgtagaca ctatatctgc atacccaagc attattacac ctatagtatc ctcatctact
   100081 aagttaaata cattaattaa ttctttccta ctcaaatcat ttttcttata tagagacaaa
   100141 agtatcatat gatatcttac atatttactt ataagtctat atgtctctgt tgataactct
   100201 aactttattc ccatattgtc aattatttgg gcacctattt tttcatgatt tgtgaaatga
   100261 actcttccgg tttcatctaa ggtcatactg tcaggcttac ctatatcatg taaaaatgtt
   100321 cccagcttta atacatttaa cttatttatt ccttcttcaa tatatgtttt taaatagttt
   100381 gacacatgtt ttcttagatg tattggaaaa aaatccttat catttaatac tatttctaat
   100441 tcttttaatg catttataga atgttcaaaa caatttacta catgatattt acattcacct
   100501 acagacttca tacagcttat tttaggaatt attttttcta attcatgctt attatctaat
   100561 tctataaata ttttatgtgt atcttccctc tttaaactct ctattataat ttttcctatt
   100621 tccataaaat cacctcagat tcaggtttta taaatctaaa acttgtttat aaaccacatc
   100681 tttctttaac tttctatctt tacaaactat ttttattgca tcttttttgt tcataccact
   100741 ttcaattagt tttaaaacat attccctatc tgtaagccca tcataatttt ctttctttga
   100801 cttctcacca tgaaaaccct ctactattaa tacaaactct cctcttactt ctttttcctc
   100861 aaatattttt atggtagttt ctatattttc tcttattatt tcttgatatt tttttgtaag
   100921 ttctctattt atagaaatat tcctattacc aagaatttta agcatatctt ttagtgtatc
   100981 cttcaatcta tgtggtgatt catagaaaat tatagttcta ctttcttcct ttaattcttc
   101041 caattgtgct ttcctaactt ttttatctct atctaaaaag ccctcaaaaa taaatctatg
   101101 tgtgtccata ccagagccaa ctaaagccgt tacaaaggct gttgcacctg gcaatacttc
   101161 tatctctatg ttattttgta tagcttgttt tattatctct tctcctgggt cagatatacc
   101221 aggcatacct gcatcactta tcagagctat attttcacca tcaattagtt tatttatcaa
   101281 ataatctccc tttgaatcct tattatgttc atgataacta gttagtggtt ttgagatttc
   101341 aaaatgattt aaaagtttta tactatgtct tgtatcttct gctgcaatca agtctacttc
   101401 ttttaatgtt cttaaagttc tatatgttat atcttctagg tttcctattg gtgttggaca
   101461 tatgtacaat ttcccgctca tttacaactc tcctatatct ttattattat atattgcttt
   101521 taattctttt gtataattcc catcttctcc ataaacatac aatgggtcca atattttaag
   101581 ctctggcctt ccatctttta caaattgaac caaaactaag ttcggtgctt tttgaggtct
   101641 tggatgcaca aattgaataa cctttggttc taatttatat tttctcccta aagttattat
   101701 atcaaccaat cttgttggtc tatgtatcat gaagaatttt cctctaggca taaccaatct
   101761 ggatgcagct ctaattacat cttccaaatt acaccttact tcatgtcttg atattgcttt
   101821 tttatcattt ggatttttta ttccatcaat atgcatatat ggaggatttg atgtgactac
   101881 atggtaacca tttatgtcta agactttgtc tatgtatttt atatcttcat ttataatctc
   101941 aactctttct tctaatccat ttaattttat agaacgagtt gccatttcat atacatcttc
   102001 ttgtatttcc acgccaataa tttttttagc ttcacttttt ccagctatca aaataggtat
   102061 aattccagtt ccagtaccca aatctactac tttggcatct ttttttattt tagtaaaatt
   102121 ggctaataaa actgcatcta taccaaaaca aaaaccatta gtatcttgta taattttaag
   102181 gccttttaat tgtaaatcat ctattctttc tgaatctttt aattgcatat ataaattctc
   102241 ctcaaatatt aataacttta agcattaatt gctttaaaaa aggtaggaaa agccctacct
   102301 taaatttaat cttctaattt ctttagctct cttagtgtgg cttcatctaa tccatcatta
   102361 tccttacatt taccaccaca gccatcacat tttttaggct ctctaagtat ttttatatct
   102421 tctcttgtgt atatctttac tgtcttgtct tgaaattcca ctttaatttg ttctaataaa
   102481 ggattcactt ctacaacttt tccttttcca tcatctgatt gcaccaatgc acctacaaca
   102541 ggcatttttt ctattgcttc actatataca tcatgctcat attttaagca acagaataat
   102601 ctcccacata ttccagatat ttttgttgga tttagagata gactttggtc ttttgccatt
   102661 tttattgaaa ctggttgaaa atcacctaac caagatgaac aacaaagttt tctaccacaa
   102721 ggccctagac ctcctataga tttagcttca tctctgacac ctatttgtct aagttctatt
   102781 ctagttttaa atatagcagc taagtctttt accaattctc taaaatctat tcttccttct
   102841 gctgtaaagt aaaaaatcaa cttattccta tcaaatgtat actcacaatc tattaaaaac
   102901 attgttaact catgttcttt tattttttgt tggcacaact caaaagtttc ttttgccttt
   102961 tccttatttt ctagatatac ttttttatct tcttctgtag ctattcttat aattggcttc
   103021 aaaggagaaa ctaattcgct ttcatccata tcttttggac caacgactac tttcccatac
   103081 tccaagcctc ttgcagtttc tacaactaca tccatatctt gctctatatt aaaatcaaca
   103141 gggtcaaaat aatatatttt cccagcactt ttaaacctta tacctactat ctttatcatt
   103201 taatcacctc atatatattt aaagacataa cttgtatgct tatattgaaa ttgcaattgc
   103261 tttttatttt actctttgca tcttctatta tatcaataat cttggacaat tgagaataac
   103321 taatttttcc actcatattt tgaatgaaaa ctagcttatc tacatttatt aacatgttct
   103381 tgtttacatt ttcttttaat aggatgatat ctcgaaaata gtttatcatc atatctaata
   103441 aagctataat cttacctcta tatttttcca tattatttgg tatatttaaa atctccacta
   103501 tgtttttatc taacattatc tgaatatact gttgtatatc ttctctcatc attgaaaact
   103561 cagaggactg agatagattt agagcattct caatacttcc tctcgaaaaa atagccaata
   103621 attgagctct ttcttcttct atacctgtat ttatcaaata acgttttaag tcttcaattg
   103681 gtataggtga gaactttatt atatcacatc tagattttat agtttctaat aaagattcct
   103741 tgttgtttgt gactaagatt attatagcat agtttggagg ttcctctagc gtctttaaaa
   103801 gtgcattttg agcttccaca gtcatttttt cagcattatt gattatatat attttatagt
   103861 ctttatgagg tcttattact atatctgatt gtaaatttct gatttgagct atttttatac
   103921 tatttccatc tggttttatg agtacatagt ctggactatt ttcgagattt tcataatcaa
   103981 gtaatagttt tgataattca tctgcaaatt tcttttttcc tattccatcc attccttcaa
   104041 acatatacgc attattaagt ttatttttct ttatagaatt tgttaaatat ttctttgcaa
   104101 aatcttgccc tataatattt tcaaaataca tatttatctc tccataggtt ttattgaatc
   104161 aagaactatt ttataaattt cattatgtat actttcaata cttcttaatt tgttatctaa
   104221 aacacaattt atttttttcc aatcatattt atctgctata tataaagcat tgttgtaaga
   104281 tttctctaaa tactctatgt cactttcatg aatatccttt ttttcttccc ctgtaatttt
   104341 attttttcta ttctccataa gctttttact aaattcaata ggcacatcta aaaatataac
   104401 acaatcaggt tgtggtatct taaataaatt aaattcataa tcaactaacc aatttaaata
   104461 ctcttctcta tcacaaatat ccattttaga ggcctgatgt accatattag aggttgtata
   104521 tctatctgcg ataattattc caccttcatt gtaaaactcc tcccaatcgg ttttaaatga
   104581 tgcataccta tcagctgtaa aaaaagttga tgctatatat gggtctacat ctgatgcatt
   104641 tttaccaaag tctcctttta ggtacatttt caccaaggca gatgatggag attcataatt
   104701 tggatattct acctttttaa tcatatagcc ttcttttttt aatctttcat ataatatttc
   104761 tgtctgagta gcctttccac ttgcatctga accactctct ataattatta acttaccttt
   104821 cattttcata tcctccatac aaacactttt tactttttta gtacttttat tagatagctc
   104881 tatataagca gtctatttat ttctcttcac aattttaaac tatctacttc tatctacttt
   104941 tatatcataa tttttaaatg tcttctaaat ctattacttg tataaaattt aaatctacat
   105001 ctttcaatcc actcacaatc atgcctttag aatgacaaaa cattagatag tctattattt
   105061 ctttacttat tacttcacca ggagatatta cacttattcc aggtgggtat ggaactatac
   105121 attcaccaca aactttacct atgctgtcat atattttaac acttttttta gttttataaa
   105181 atgcttctct aggtgttaat attttctttg gaataacaac agggtatttt ttattttcta
   105241 acttttcaaa tttaaagtct tcctttacaa cattgtttaa tgctgtttct aaactagaga
   105301 aatcctcagc agtatttcct attgtacata ttaaaagaac tccataataa ttagataact
   105361 ccacttgtat gttatattta tttctcaaaa tattttctag ttcataacct gttaaaccca
   105421 tgtttttaga tgatataaat atttttgttt tatcatttgt gtcgtatata ttaatatttg
   105481 aatcaacttt atttttgaaa gttataatat tatttaaaag ctcttccatt aattcttttc
   105541 ctttattttc atatatatct actgctaatt ccagagaaga taataataaa tatgaagggc
   105601 ttgaagattc tgtaattctt aacatgttca atattttatc tatatcaact cggtctccct
   105661 gtatatgtat cattgacgat tgtgtaaatg ctggaagagt tttatgtgta ctctgtacaa
   105721 ctatatctgc accttgctca agagctgttt taggaagcct ctcgctaagc cctaaatgcg
   105781 caccatgtgc ttcatctact atcactacca tctttttgct atgtgcataa ttacatatgt
   105841 attctaaatc atatgtcatt ccataatatg taggataagt aagcagtact gcttttgcat
   105901 ccaagttttt atctattata tcaatcacat tacttataga tatccctttt aagacatttg
   105961 tctcttcact tatctcacaa ggcacatact ctatatctat atctcctaaa atacatccat
   106021 ttataacaga ctgatgacag tctctatttg ttattatttt acttttagga ctacatactg
   106081 acattattgc agattgtatt ccacagctac taccatttac taaatagcaa gtataatcac
   106141 ttttaaatac tcttgatgct tttttttgtg aattttttat aattccttcc ggaaagtgta
   106201 aattatctgt tcctaatatc tcagttgtat ccattttata caaatttttt agtatatctt
   106261 tatatcctaa ctttttataa acctgtccct ttttatgacc aggcatatga aaagatatta
   106321 cttcttcatt tacaatctta tttaattcat taataataaa catatattaa tagtactcct
   106381 tttttacata atacttcttt ttaaaacttc aattcttatc tattttacca ttaaaaaaga
   106441 aacaaattaa tatttttata aaattaaata cctcattcta aacatttaga atgaggtatt
   106501 taacataaaa gcataaatta ttctctatca tcagacatta aacttggtat taagtataat
   106561 ccagctaaac ctacaatgaa gtatattatt ctacttacca tttcatatgt tccaccaaag
   106621 atactaccta ttaagtctgt acctagaata ccaattgcac cccagttaag cgcacctatt
   106681 atagctaaaa ttaaagcaac ttttttcact tatatcactc ctcaagaatt aaatttttat
   106741 aaagtccata attcctgtgt ttagttttcc cgaaatgaaa aaagaaatac gtattttttg
   106801 ttttttttga caaaaaataa aaaa
//
LOCUS       DBGJFF010000003        95418 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.003, whole genome shotgun sequence.
ACCESSION   DBGJFF010000003 DBGJFF010000000
VERSION     DBGJFF010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 95418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 95418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..95418
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.003"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(<1..1338)
                     /locus_tag="ACOIAF_000271"
     CDS             complement(<1..1338)
                     /locus_tag="ACOIAF_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HGZ2667307.1"
                     /translation="MEKAKKLGLFSMILLGINSIIGSGIFLLPGKVYNLAGQNSMFIY
                     IFATLLVLSILLCFAEVGSMFDKNGGAYLYSKKAFGDFIGFEVGTMSWVIRIISWSTL
                     AVGFATALGSFWPESATEYKGYIAAILVTLLSINSLFGIKSTKIMNNVITIAKLVPLI
                     VFIIVGIFFIKFVNIVPSGNVVNSSMGPAIILVFYAFTGFESFIVASGEMENPKKNLP
                     VALITTIFICAIIYILIQIVCMGILGDRLFENSIPIADASSVFVGNYGKVFISVATLI
                     SIFGINIGSSIVTPKCGSSLAEEGSLPAFIGKTNKYGAPYVAIIISLICCIPLVLTGS
                     FEQLAVMSVIARFAQYIPTCLSVIVLRKRTDVKASFKIPFGPVIPIVAILGSLWLLQQ
                     AWAEDISKPITQNRVLIGLGAMLLIAPLYIFMKKNKEIEHKNKSEDSKLYISRE"
     gene            complement(1375..2595)
                     /locus_tag="ACOIAF_000272"
     CDS             complement(1375..2595)
                     /locus_tag="ACOIAF_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HGZ2667308.1"
                     /translation="MQQALKLKYQTKELGKDFVIESCNSIKPWLINIRRELHKIPELA
                     LEENLTKQKVISYLKEIGIDYMEFTKHNGIMAYILKESADKTICIRADMDALPIEEEN
                     NIPYKSIHSGKMHACGHDAHTTMLLGACKVLHSIKDKLNVNVKFLFQPAEEGFGGAKF
                     LVEDGCLENPKADYIFGLHVMPHIETGLIETKYDTLNASVDTIKISIKGKRAHGAYPE
                     NGIDAIVTASQIVTSLQTIISRNLEPNNAAVLTIGKIYGGDAHNVICEDVKLEGTLRT
                     LNSKTRNFMIDKIAKIVGDTASAFGCVGTLHVSDENYPAVINEKELVDTVISNTKELL
                     GEEKFILRPNPSLGGEDFSFYTEHCKGAFFHLGCKNEEKGLISPLHTSSFNIDEDCLP
                     IGVMMHVMNTLYFN"
     gene            complement(2964..4976)
                     /locus_tag="ACOIAF_000273"
     CDS             complement(2964..4976)
                     /locus_tag="ACOIAF_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HGZ2667309.1"
                     /translation="MKKSVALVNDSRKDLIDFLENNLKLVFGDSININRYFINEINDN
                     DIINDDVILVMSVERLDKIINNILDKKKVIVVRRTFREDKIYNLLSLPQGTNVLIVND
                     SDETTLETISLFYKIGVTNIRPIPYMNDNNYKNIKIAITPGVPEKVPSFISDIFDLGH
                     RYIDISTFIEIINLLQIDSKEIQSNLVKYSEEIISLDTGIKDKYKELFLKIEELDTIL
                     NLSKDGILFTSKDGEINTYNSKVKDILDINEDIYGKYIEDIFVDSLKVLLSEKEILDK
                     VVVFNKKYINVNKKNIYNRDEKMGTYYSLQEITYIKKLEQNLTKKLREKGQIAKYTFK
                     DIKTNSPKMFECIDLAKKVSKSDLSILIRGESGTGKELIAQSIHNNSNRKNQPFIAVN
                     CAAVPENLLESQLFGYDKGTFTGGLKDGKQGLFELANNGTIFLDEIGDMPLELQTKLL
                     RVLQEKQIMPVGSHNVINIDVRIISATNKNLEQMIDNSQFREDLYYRLNTIPINIPPL
                     RERKEDILIIMEDLINKKLVITPEAKKLIQNYMWKGNIRELQNVTSYLNIMCEDIVLE
                     KDLPPNLRSSDNKNTSLKLKYSKNDILNILEILILNKESDVGIGRGLILKALLDKNLQ
                     ITEGKIKKIFEYLKKEELIICSSGRYGSKITQKGEDFYNKLKYKGL"
     gene            5447..6097
                     /locus_tag="ACOIAF_000274"
     CDS             5447..6097
                     /locus_tag="ACOIAF_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HGZ2667310.1"
                     /translation="MKAEKTSIYPIIKNNLNKISIVEYKKGQRFVASDKDLQEVFFII
                     EGVALVECTTRGGNKFLVDIVPENEFIGKISYIYEHNLKCDIFAKTNIKLFRFEKNVF
                     EEFYLKPDFIALFHRKCTRRIYELYKTRMVRELFSCTEVIAYCISNKQENNVCNIKAI
                     RNFSEIYSISRKSRYHSLLKLTEKEIVKKMGNTYEILNYDELHKLAFEVKEFLEDE"
     gene            complement(6237..7613)
                     /locus_tag="ACOIAF_000275"
     CDS             complement(6237..7613)
                     /locus_tag="ACOIAF_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HGZ2667311.1"
                     /translation="MQELFSLKDENKRFYKILLSLCIPIIIQNLISTSVNVIDTIMIS
                     SLGETSVASVGVANQFFFLFNMSLSGITGGAGVFISQFYGKKDVSNIRKVTGLTCVLA
                     IVLSFVFVIPALLTPKPIIHIFSYDSEVVKLCIDYFSIAVFSYPLIAVSTVFSTGSRG
                     VRNPKLGMICSAFALVTNVILNYGFIFGNFGLPALGVKGAALATVIARICELILMITY
                     VYLYKKDYILKFGLKNLKAIDKIFIKSFSSKSFPIFVNDSVWAIGTVLYSVAYARAGT
                     SAIAASQIATSTGNFFIMTAVCIASGASIMLGNELGADHIKRAIEYAKKFSILVFSAG
                     LILGIILILNIPLLLKMFSVSDSLASDITKIFFIMGILMALKSFNTLVIIGILRSGGD
                     TKYALFLELGCMWLASIPLTFIAAFKGAPIFVLVLLTYSEEVVKFIFGVPRALSKKWA
                     INIVKEID"
     gene            complement(7629..9542)
                     /locus_tag="ACOIAF_000276"
     CDS             complement(7629..9542)
                     /locus_tag="ACOIAF_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HGZ2667312.1"
                     /translation="MIVLSCNNLNKSFGIDSILENISFTVNEGDKIGIIGVNGTGKTT
                     LFKIISGIYGYDSGDIYTSKDCEIGYLEQNTNFYSDNTILEEVLEVFKNLIEMESYLR
                     ELEVKISEESTKTNSPIIEKIMDEYSHKLELFSDLNGYGYKSEAKGVLKGLGFSDNDM
                     DKPISILSGGEKTRVLLGKLLLKKPTLLLLDEPTNHLDSEAIEWLEVFLKQYKGTVML
                     ISHDRYFLDQSVNRIFEVHNKKLKVYNGNYSKFVELSKIEKELELKKFEDQQKEIKKQ
                     EESIERLKAYGREKHLKRARSKEKALDKVDVLDKPEAYRKKARIQFTPSVQSGNDVLQ
                     IRDVSMGYGERILFKDLDLDIYRGEKVALIGANGVGKSTLFKIITNELQPLSGNIKFG
                     TNVHVSYFHQEQKTLNLDNTIIDEIWENNTHLTQTTLRNMLGAFLFVDEEVFKKISTL
                     SGGERARVAILKLILSNANLLLLDEPTNHLDIDSKEVLEEALTNYDGTIFTISHDRYF
                     LNTVVDKILVLDENGITEYLGNYDYYIDKKRQIQEMSIIEEKEEKTRTQIKDEKRKER
                     EQREIEKKNRIKRQNIEKEIEKLEIEIEKLDILLCQEEVYSNPDKAKEVSQEKINLEN
                     NLASLYDEWEEFM"
     gene            9678..10310
                     /locus_tag="ACOIAF_000277"
     CDS             9678..10310
                     /locus_tag="ACOIAF_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HGZ2667313.1"
                     /translation="MLGNKNISMAVIRRLPKYHRYLGDLLDRDIQRISSKELSDIIGF
                     TASQIRQDLNNFGGFGQQGYGYNVEALHTEIGKILGLDRPYNAVLVGAGNLGQAIANY
                     AGFRKAGFEIKALFDANPRMIGLKIREFEVLDSDTLEDFIKNNNIDIAVLCIPKNGAQ
                     EVINRVVKAGIKGVWNFAPLDLEVPKGVIVENVNLTESLFTLSYLMKEGK"
     gene            10449..10988
                     /locus_tag="ACOIAF_000278"
     CDS             10449..10988
                     /locus_tag="ACOIAF_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HGZ2667314.1"
                     /translation="MVFVEELWKKAIEFHGHECPGLAIGFKAALAAKKYLGCKQSGDE
                     EIVCISENDACGIDGIQVVLSCTMGKGNLLLRMTGKSVYHFFNRENGKSIRIYVKSKP
                     FEMDREGYKRYLLESDFEEIFEITDTKLRLPEKARIFKSLKCEVCGEFASEQYIRIEE
                     GKKVCLDCFDKYERFYEEY"
     gene            complement(11164..11334)
                     /locus_tag="ACOIAF_000279"
     CDS             complement(11164..11334)
                     /locus_tag="ACOIAF_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HGZ2667315.1"
                     /translation="MAYKITDACISCGACEAECPVSCISAGDDAYVIDAGSCIDCGSC
                     AGACPVDAPQPE"
     gene            11619..12686
                     /locus_tag="ACOIAF_000280"
     CDS             11619..12686
                     /locus_tag="ACOIAF_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HGZ2667316.1"
                     /translation="MKNKLVYICIMSLLLFVVGCSNNPNKESTIKKDSKNNQSQVKEE
                     KREPVKKDITISFAGDVTMGNYKGSSYYGSFDHEFKRQGEDYNYFLKNVKPVFEKDDL
                     TVVNLEGPLTTASNAKEKKFAFKGKPGYVNILKSGSVEAVSIANNHSEDYFEEGMKDT
                     QFILDENKINYFGLGKDAVVDVKGIKVGLLGYNGLSTEYNEKNLKQMEDDIKSLKKKS
                     DVVVVYFHWGIELDYYPDKKQKDFAHYAIDKGADLVMGSHPHVIQGIEKYKNKYIAYS
                     LGNFCFGGNKNPSDTDSYIYQQTFTFSDNKLTSIKEPNIIPTSITSSNSRNNYQPKIL
                     DGSEKDRVLNKLEKISKDLNK"
     gene            12856..14757
                     /gene="cooS"
                     /locus_tag="ACOIAF_000281"
     CDS             12856..14757
                     /gene="cooS"
                     /locus_tag="ACOIAF_000281"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_function: GO:0043885 - anaerobic carbon-monoxide
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006091 - generation of precursor
                     metabolites and energy [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HGZ2667317.1"
                     /translation="MACNTCKMCESADKKLESFVASKDVETAFHRTEDQKVKCGFGLQ
                     GVCCRLCSNGPCRVTPKSPRGICGADADTIVARNFLRAVASGAACYLHVVENTAKNLK
                     NVGITKGVVKGEKTLNQLAEMFGIESDEKYDKCIKVADKVINDLYKSRDDKMELVEKI
                     AYAPRVKKWKELGIMPGGAKSEVFDAIVKSSTNLNSDPVDMLVNCLNLGISTGLYGLT
                     LTNLLNDVMLGEPVIRMAPVGFNVIDPDYINIMITGHQHSTFANFQDRLKDEDVIKLA
                     QSVGAKGFKLVGCTCVGQDLQLRGEHYQEVFAGHAGNNFTSEAVLSTGAIDIVLSEFN
                     CTIPGLEPIADKYKVKMVCLDDVAKKANAEYIGLDRSKLDELSNTLIEKALESYKERR
                     GSIEIDIPKDHGFEQSLTGVSEKNLKAFLGDSWKPLINLIAEGKIKGVAAVVGCSNMT
                     AGGHDVNTVELTKELIKKDIIVLSAGCSTGGLENVGLMSPGAEELAGENLKEVCKTLG
                     IPPVLNFGPCLAIGRLEIVATELAAELGIDLPQLPLVLSAPQWLEEQALADGAFGLAL
                     GLPLHLALPPFITGGKLVTEVLTEKLKDLTGGHVIVNPDPKSSANQLEEIIIDRRKNL
                     GLKDVECNA"
     gene            14750..15223
                     /locus_tag="ACOIAF_000282"
     CDS             14750..15223
                     /locus_tag="ACOIAF_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HGZ2667318.1"
                     /translation="MHRIFINKDLCTGCKSCVLACMLKHNKDYDMYTLDLENIDNDSR
                     GHIELDSRHNNPVPILCRHCDEPECVLACMSGAMHKDSESGIVSYDEEKCGSCYMCVM
                     SCPYGLLKPDDRSKQNILKCDLCKDEEYPRCVANCPSGAIELQKEENDELCSVRG"
     gene            15198..16424
                     /locus_tag="ACOIAF_000283"
     CDS             15198..16424
                     /locus_tag="ACOIAF_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HGZ2667319.1"
                     /translation="MNYVVLGASAAGINAVKTLRELDKDSNIVVISKDENVYSRCMLH
                     HVISEHRTLKQINFVDEDFMEQNNVKWIAGKTVKGIDINKKVVQTEDITVNYDKLLIA
                     TGASSAIPPIKNLRDGNFVYSVRNIDDIYKIKEKAQNSKNVVIIGAGLVGIDALVGLF
                     KYEQLNISVAFMEKYILDRQLDEYTASVYENKFKEKGVKFYPSASIQEIVLDNSKNVT
                     GVAFSNGEVLDADMVIVATGVKPNADFLDGTGIEYDRGIIIDDMCQTTQKDIYAAGDV
                     VGKNAIWPLAVKQGIVAAYNMVGKDKKIEDEFAFKNSMNFMDIPTISIGMNTPVDDSY
                     KVLTRHGINDYKKFVFKDNVIYGAVIQGDISYVGVLTYLIKNKVEIYDLENRIFDICY
                     ADFFNIKENGEFCYSV"
     gene            complement(16563..17750)
                     /locus_tag="ACOIAF_000284"
     CDS             complement(16563..17750)
                     /locus_tag="ACOIAF_000284"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGZ2667320.1"
                     /translation="MNITKNFLKDYLEKFGETPFLVRLKDEEEFLIGEGFPQFKVIVN
                     NDISKSELLKSTSLALGEAYINRYIEVEGDLFLTLNLLLKQIDKFNIDNSAVKHLLHS
                     SNSMRHQKKEIHAHYDIGNDFYSLWLDKTLSYSCAYFRNDNDTLYDAQVNKVHHILTK
                     LNLEEGMSLLDIGCGWGFLLIEAAKKYKIHGVGITLSEEQFKEFNARIKEENLQDYLE
                     VRLMDYRELKKSDLMFDRVVSVGMLEHVGRENYELFIKNVNAVLKDGGLFLLHYISGL
                     QESYGDPWIKKYIFPGGVIPSLREMIHICSDYKYHTIDVESLRLHYVKTLLYWQKNFS
                     ENLPKIKEMFDDKFIRMWDLYLCSCAAAFNTGIIDIHQILFTKGIKNDLPLTREYMYN
                     CSK"
     gene            18023..18847
                     /locus_tag="ACOIAF_000285"
     CDS             18023..18847
                     /locus_tag="ACOIAF_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HGZ2667321.1"
                     /translation="MEVYALVGSSGTGKSFKALEFAYENDIEYIIDDGILIYKNKVLA
                     GISAKQANTTIEAVKRAIFYNLEHRQEVREAIKKENIRKILIIGTSKKMVNQIVERLS
                     IGRVCKFINIQDISTKYEIEIAKQARKEGNHVIPVPAVEIKSMASGLSINSLKRLFRK
                     GNNRNMVVLEKTIIRPTFSYIGKFYISADVIKQIIEYEVYNFGSIDRINKINIENCNN
                     FMNIFVSININDLETIKKIEDIQQTIKKSIEKMTLVNVQKIDINIHKIKKINFLQC"
     gene            18976..20241
                     /gene="gluD"
                     /locus_tag="ACOIAF_000286"
     CDS             18976..20241
                     /gene="gluD"
                     /locus_tag="ACOIAF_000286"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-specific glutamate dehydrogenase"
                     /protein_id="HGZ2667322.1"
                     /translation="MSGKDVNVFEMAQSQVKNACDKLGMEPAVYELLKEPMRVIEVSI
                     PVKMDDGSIKTFKGFRSQHNDAVGPTKGGIRFHQNVSRDEVKALSIWMTFKCSVTGIP
                     YGGGKGGIIVDPSTLSQGELERLSRGYIDGIYKLIGEKVDVPAPDVNTNGQIMSWMVD
                     EYNKLTGQSSIGVITGKPVEFGGSLGRTAATGFGVAVTAREAAAKLGIDMKKAKIAVQ
                     GIGNVGSYTVLNCEKLGGTVVAMAEWCKSEGSYAIYNENGLDGQAMLDYMKEHGNLLN
                     FPGAKRISLEEFWASDVDIVIPAALENSITKEVAESIKAKLVCEAANGPTTPEADEVF
                     AERGIVLTPDILTNAGGVTVSYFEWVQNLYGYYWSEEEVEQKEEIAMVKAFESIWKIK
                     EEYNVTMREAAYMHSIKKVAEAMKLRGWY"
     gene            20454..21044
                     /locus_tag="ACOIAF_000287"
     CDS             20454..21044
                     /locus_tag="ACOIAF_000287"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HGZ2667323.1"
                     /translation="MHFKSKDLNLKYLVLFLKIVYWFNPFICIMEKYMDLDCELYCDE
                     RVLKNCTVQQKQEYALTIVNSMKRSTNFSNKFVAGLNKQSDIRKRVINMFSEKHKNGI
                     LRALIISLFSSITFFKFDSTSIIEENDFLERSRYTFEGRQEDMFVTIDFIYADVPIKL
                     RKEHEENCRVNGIVPKDSDSIEIIPRFYKDCMDLKK"
     gene            21374..22507
                     /locus_tag="ACOIAF_000288"
     CDS             21374..22507
                     /locus_tag="ACOIAF_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HGZ2667324.1"
                     /translation="MSNFILNGIFRTVIVSSLSILLILIFKKNVFKRFSKKFNYYIWM
                     IVVIKLFLPFTYYTFTINILRSKKHININNINLEGFNNVSTISNNIILYTWIITVVVY
                     LIYTIFKYIKLKNLINDLSYDVDDREIVNLYKNILEEFNITKDIKLKYSYEVETPAFF
                     NSCVLLPPHEYKLKELDWIFRHELMHFKSRDLYLKYVILFLKTVYWFNPFIYIMDKHI
                     DLDCELYCDERVLKNRNSEEKKDYALTILNAMRKGSNTSNKFIAGLHKQSDIKKRVMN
                     MFNEKYKNGILMALTLCLLSSITFLKVNSTSIVNLNVLPKGFEDLRTVSSIRFSVIGF
                     TYADAPKNMREEYKINCESLGLIPKDSDKIEVGIEYYDSLLND"
     gene            23423..24445
                     /locus_tag="ACOIAF_000289"
     CDS             23423..24445
                     /locus_tag="ACOIAF_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HGZ2667325.1"
                     /translation="MKKKILIPVFASVMALSVSSIVNADEVNDSSQNKDDKTNTELNL
                     GEYKEVKYKVAKIKDGVAIKIREEGQVQNIAYSGDEFTVLGTQGEWVKVKVEDGEGWL
                     ATRYVDISEGVGYTNADKVNLRKDKSESSEVIEELEKGSSLLVLEDNGDWLKVKDGET
                     EGYVKSSYISDKAPVIEEPQVNPSINQNVDSTNTNQQSNANTNQQNNNSNVPTANSNA
                     VQAVLNLAYSKQGCPYVWGAEGPNTFDCSGFTQYVYRNAVGKNIPRTSKAQSKYGQTV
                     SKANLQPGDLVFFTTNGSGSVSHVGIYVGGGNMIHSPSTGKTVSVTSINSSYYTARFV
                     TAKRIL"
     gene            24742..25662
                     /gene="pyrB"
                     /locus_tag="ACOIAF_000290"
     CDS             24742..25662
                     /gene="pyrB"
                     /locus_tag="ACOIAF_000290"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HGZ2667326.1"
                     /translation="MLKSRNLIQPEDFSIEEIDEILELAQKIIDNPSKYSRICEGKLL
                     ATLFYEPSTRTRLSFESAMNRLGGRVVGFSEPNSSSASKGETLGDTMRIVSGYVDIIA
                     MRHPQSGAASEAARYTEVPFINAGDGKNQHPTQTLTDLLTIKSLKGTLESHTIGLCGD
                     LKYGRTVHSLVKAMARYKNTKFVFIAPEELKMPDYIKDAIKGHAYYETNNLDDVIGSL
                     DVLYMTRVQQERFEDKSEYERLKNYYILNKVKLEKASKDMLVMHPLPRVNEIDIDVDS
                     DDRAVYFKQAKYGMYVRMALIIKLLGISED"
     gene            25701..26393
                     /locus_tag="ACOIAF_000291"
     CDS             25701..26393
                     /locus_tag="ACOIAF_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HGZ2667327.1"
                     /translation="MYKILENIYIGEDMYRMKVKGNFEGKMGQFYMLRAWDTYPVLSR
                     PISIHDIDEEGITFLYKVVGEGTQILSNLKVNDTIKLEGPYGNGYAKVDGKVALVGGG
                     IGVAPLYLVAKNIKNCDAYLGFREDVILENEYKQVCNKVYTTVGNTFVTDIIDVEKYD
                     YILTCGPTPMMEKLVKMVEGTKTRIMVSLENHMACGVGACLVCTCKTNEGNKKTCKDG
                     PVFWGEDVIFNG"
     gene            26386..27288
                     /locus_tag="ACOIAF_000292"
     CDS             26386..27288
                     /locus_tag="ACOIAF_000292"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HGZ2667328.1"
                     /translation="MGNLSVKFGSVEFKNPVIMASGTFGFGKEYNEIYDIQKLGGISS
                     KGITLNKKPGNNGMRVHETPSGMMNSVGLENPGVQGFIDNELPFFSKLDLVRIANVGG
                     GTLEDYLLGVQMLNDKPIDIIELNISCPNVKAGGMAFGIKNKVAREVVREVRNITKLP
                     LVIKLSPNAEDIVGMAKVCEEEGADGVSLVNTFKAMAIDIKNRRPVFENVYAGLSGPA
                     IKPIALRMVHEVCKNVNIPVMGMGGITKATDAIEFIMAGATCIQVGTANFMNPRIGIE
                     IIDGINEFMDREGIKSLDEIRGII"
     gene            27373..27957
                     /gene="pyrE"
                     /locus_tag="ACOIAF_000293"
     CDS             27373..27957
                     /gene="pyrE"
                     /locus_tag="ACOIAF_000293"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HGZ2667329.1"
                     /translation="MSKVDVVDILKKSDALLEGHFLLSSGKHSNRYVQCAKVLRFPEY
                     AAEVLSTVVEQIKDLDIDLVVGPAMGGVIVSYELGRQLGKEAVFTERKDNTMELRRGF
                     EVKKGAKIIIAEDVVTTGKSTMETKRVLEALGGEVVGVACIADRTNHDIGMPIYSAIK
                     LDIQVYESDECPLCKEGKLPVVKPGSREFKELGM"
     gene            28173..29099
                     /locus_tag="ACOIAF_000294"
     CDS             28173..29099
                     /locus_tag="ACOIAF_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HGZ2667330.1"
                     /translation="MKNQLNENHKKVVFFGVGAVGATFAEQFFNSKYDFKILCDNERK
                     KRYLEEGFIINGKRYDFDYVTKDEYKQEADFIIIGLKYNNLKENIKELDGLVGKNTVI
                     MSLLNGVDSEEIIGERFGIEKMVYSYVTNIDAKKINNNIIHTTNGVIVFGNKDNSEDR
                     KTNIITEVFDDVNIEYILSKDIQRDMWWKYMVNIGVNQTSAILGAPYGVFQSSEHLRE
                     LAKSAMREVVAIAQAKDISLTEDDVENSLHRILEHSKEGRTSMLQDVEAHRLTEVDMF
                     SKNICKLGKKYNIPTPINQTFFYMIKVIESRF"
     gene            complement(29151..30041)
                     /locus_tag="ACOIAF_000295"
     CDS             complement(29151..30041)
                     /locus_tag="ACOIAF_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGZ2667331.1"
                     /translation="MFEELKTFVAVVEYKNFTKAGEYLNLSQPSVSKHIKNLESYFKV
                     VLINRSIKQKTIFITESGQILYKRAKEILNLLNITYHDVSQVSDAITGHLKIGASLTI
                     GEYILPNFLALFSKKYPDIDVEVFIKNTSIVSSHVKDYILDIGLIEGTCSSPSFIQEY
                     FFEDKMVLALPYKSHLLKDFSFDKLQNQKWIVREDGSGTRDYLDMFLSVKEIIPKSMM
                     VFGSNYAVKESVRNNLGITIVSNLVTSLPVLNNELSVIELGSSYNRHFSYIFPKDITL
                     SKAATIFIEELKIFSNLNSI"
     gene            30229..31260
                     /locus_tag="ACOIAF_000296"
     CDS             30229..31260
                     /locus_tag="ACOIAF_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HGZ2667332.1"
                     /translation="MLKKKSSLTLKTIREILPGLFVSVLVGYISIFISILIPKVGAAS
                     ISIFLGMFVGNLFLNQKVFQKGYKFSETDLLSYSIVLLGATLSVSTLIDLKVSGILFI
                     ILQMTITIIAALYIGKKLGFEENFRFLMASGNAVCGSSAIGATVPVVNATDKEKGIAV
                     TIVNVTGIFLMFLLPIISQFLYSHELVKTSAMIGGTLQSIGQVVASGAIVGENVKDLA
                     TIFKIVRVIFLVVVVLVFGHMKNQSNREILEEEKDEIKKKKVTIPWYVIGFFITCALF
                     SMNIIPKEVSVLCKEISNKLEIIALAAIGLKVNVKDLVKQGKEVSLYGLFVGTVQVVS
                     AVVLIKIFI"
     gene            31554..32429
                     /locus_tag="ACOIAF_000297"
     CDS             31554..32429
                     /locus_tag="ACOIAF_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HGZ2667333.1"
                     /translation="MNVYEKGNGVILEGVTDFDPVHIFECGQCFRWHKQKDGSYTGVA
                     KGRILNVKKENDKIYLNNTNLKEFNSIWYNYFDLGTDYTEIKNKLKNMDEYLNKATEF
                     GWGIRILRQDGWEMLISFIISSNNRIPMIQRAIENLSRKFGKYIGEYEGNEYYAFPTP
                     EELNKASQEEIRACQTGFRDKYIKSTTQEVIENNDKVSEYTNLSTEDCRKDLLKFNGV
                     GPKVCDCIALFGMQKYDSFPVDVWVKRVMQEFYIDEDMSLPKMRTYGIDKFKEMSGFA
                     QQYLFYYARELGIGK"
     gene            32447..33937
                     /gene="cls"
                     /locus_tag="ACOIAF_000298"
     CDS             32447..33937
                     /gene="cls"
                     /locus_tag="ACOIAF_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HGZ2667334.1"
                     /translation="MGVIGTIFLFYLIISYLAGAIISVIILLENRDPAKTMSWLLMFI
                     IFPGVGLMIYAISGRNIRKRKLFKTQKLANNIKEKKLFDTLEKITEIVELEKESIKQN
                     KLLRDEEDGSYRKRVINMLLKTGMFPFTKNNKVDVFVDGNEKFKRLIEDIREAKDHIH
                     LEYFIIKDSEIGRLLKEELIKKAKEGIKIRILYDDVGCWRFWFNRKFFREMREVGIEI
                     AAFLPTKFPIIGGKLNYRNHRKIVVIDGIIGYTGGINIGDEYLGKNDKFGYWRDTHIR
                     IKGISVYMLQMTFLIDWYYTTKEVLVTKNYFPSVGNIGESMIQVVASGPDSDWEDIHY
                     AYFSAICQARKNVYIETPYFIPDESLLKAIKSAALSGVDVRIIFPKIADHKIVNIASY
                     SYFEEILRAGGKVYLYNKGFIHSKVVIIDDKIASAGTANMDLRSFMLNFEVNAFIYDE
                     EVIRVMTDDFFEDLSHCEELNLEVFKNRNIIQKIKESVARLFSPIL"
     gene            34130..34414
                     /gene="groES"
                     /locus_tag="ACOIAF_000299"
     CDS             34130..34414
                     /gene="groES"
                     /locus_tag="ACOIAF_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HGZ2667335.1"
                     /translation="MKIRPLADRVVIKKVEAEEKTASGIVLPGAAKEQPQIAEVVEVG
                     PGGIVEGKEIKMELTIGDKVIFQKYSGTEVKIEGQEYTILRQSDVLAVIE"
     gene            34447..36075
                     /gene="groL"
                     /locus_tag="ACOIAF_000300"
     CDS             34447..36075
                     /gene="groL"
                     /locus_tag="ACOIAF_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729227.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HGZ2667336.1"
                     /translation="MAKEIKFSEETRRALEAGVNKLADTVKVTLGPKGRNVILDKKFG
                     SPLITNDGVTIAKEIELEDRFENMGAQLVKEVATKTNDVAGDGTTTATVLAQAIIREG
                     LKNVTAGANPILLRKGIQKAVTVAVEELKNQSRIVETQEAISQVASISAGDEEVGKLI
                     AEAMEIVGKDGVITVEESQTMNTELDAVEGMQFDRGFVSAYMVTDVDKMEAVLNDPYI
                     LITDKKISNIQELLPVLEQIVQQGKKLLIIAEDVEGEALSTLVVNKLRGTFDVVAVKA
                     PGFGDRRKEMLQDIAILTGAQVISEELGYDLKEADLSMLGRASSVKVTKENTTIVDGS
                     GDKKSIEDRVTQIKHQVEQTTSDFDREKLMERLAKLAGGVAVVKVGAATEVELKERKL
                     RIEDALNATRAAVEEGIVAGGGTAFVSVIPAIGTLIESLEGEVKLGAQIVKKALEEPL
                     RQIAINAGLEGAVIVQNVVNSEAETGFDALNEKYVNMIEAGIVDPTKVSRSALQNAAS
                     IASTFLTTEAAVADLPEKEDAGMPGMGGGMPGMM"
     gene            36337..37077
                     /gene="larB"
                     /locus_tag="ACOIAF_000301"
     CDS             36337..37077
                     /gene="larB"
                     /locus_tag="ACOIAF_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HGZ2667337.1"
                     /translation="MDLRKLLEEVKNENIDIDFALEKLKDLPYEDIGYANIDHHRELR
                     NGYPEVIYCEGKSDEHILGIIDKMNQKSSNILGTRCRKETFLKIKEIYNHAEYEELSK
                     ILKIQNHDIENIGKGKIVIATGGTSDIPVADEAYHTAKFLGNDVDRIYDVGVAGIHRL
                     LNKRHKIDGARVIVAVAGMEGALASVIGGLVDVPVIAVPTSVGYGANFGGLAALLAML
                     NSCASGISVVNIDNGFGAGYLAAMINKL"
     gene            37212..38344
                     /gene="larC"
                     /locus_tag="ACOIAF_000302"
                     /pseudo
     CDS             37212..38344
                     /gene="larC"
                     /locus_tag="ACOIAF_000302"
                     /inference="COORDINATES: protein motif:HMM:NF014072.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
     regulatory      38404..38505
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            38726..40261
                     /gene="guaA"
                     /locus_tag="ACOIAF_000303"
     CDS             38726..40261
                     /gene="guaA"
                     /locus_tag="ACOIAF_000303"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HGZ2667338.1"
                     /translation="MKHELVLVIDFGGQYNQLIARRVRENNVYCEILPCTASIERIKE
                     KNPKGIIFTGGPNSAYLEDSPTISKEIFELGVPILGICYGIQIMSHVLGGVVRKGNKQ
                     EKEYGKTAITYGKSSLFEGITTNSVWMSHTDLIEKVPEGFTIVANTNDCPVAAMENVE
                     RNLYGVQFHPEVEHCLEGDKILTNFLYNICKVKGDWTTDSFIEDKIKELKEKIGDKKA
                     LCALSGGVDSSVAAVLIHKAIGDNLTCIFVDHGLLRKNEGNDVERIFREKFDINLIRV
                     NAEDRFLSKLKGVSEPEAKRKIIGEEFIRVFEEESNKLGKMDFLVQGTIYPDVIESGH
                     GNAATIKSHHNVGGIPEDVDFQEIVEPLRELFKDEVRKIGLELGIEEGLIFRHPFPGP
                     GLGIRVIGDVTKEKCDILREADAVYMDELRKAGLYREIWQAFATLPDVKTVGVMGDER
                     TYAYLVGLRAVTSSDGMTSDWYKMPYDVLERISNRIINEVDGVNRVVYDITSKPPGTI
                     EWE"
     gene            40839..42230
                     /locus_tag="ACOIAF_000304"
     CDS             40839..42230
                     /locus_tag="ACOIAF_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HGZ2667339.1"
                     /translation="MRNKIIIGITFSLVLLMSGCASKQTAEINVLATTDLHGVIPYEL
                     TSYVKEERKKDKNLTLVDAGDFFDSGEVFGSSMDSYFSARYKDGDNNTESYIETPISK
                     DMKEVGYDVAVLGNHEFISNNKFYLDNMISDFEKQKINILSANTYKKNGDSYTKPYII
                     KNINTPEGNVKLGILGLTIKEVGERKQWKDGKLVDSKSLELKDQEGYNGELYMNDLVE
                     DAKKWVGIMEKEKPDIIVAVAHTGEKPKKPKNPGNRIQDLAQQVDGIDAIVAGHNHVQ
                     IEQHDYKNKSGENVIVTEPGKHGECISKINFKLEKNNDKWNIVDKSSKLIKFEKNQES
                     DKFGEFISSINKVSKDKKEIRLSEVKTPEWDKIYVFKSGTSADDIYKTIGYKWRNISS
                     PKGDDMVQIVVMNNNKPVSYVYGEAEVMPINIKCDESEYKDNVVTIYPNKNDKFKIEK
                     GKQEYSINLKHIQ"
     gene            complement(42378..42686)
                     /locus_tag="ACOIAF_000305"
     CDS             complement(42378..42686)
                     /locus_tag="ACOIAF_000305"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HGZ2667340.1"
                     /translation="MLREQGSAIRNTLDSVLSLSNQKAYPVWESVNSFALIKAAQAGL
                     GITILPENLLLDSLLHKKLRLIELDGIDMENKMLAILHKDKNITQPLKIILDNISNVL
                     "
     gene            42723..43244
                     /locus_tag="ACOIAF_000306"
     CDS             42723..43244
                     /locus_tag="ACOIAF_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HGZ2667341.1"
                     /translation="MFSIGGGYAIILLIQEKVVNQTGWISEKMFTDIITISQMTPGPL
                     AVNTSTFVGLQIGGFWGAVAATLGCVLCGVVISLFLYNFFQKHQNSTYIFEILNGLKS
                     ASLGLIISAAATIIILALFGTNKVYFNSIFEMLNWTALIIFFVTLLIVRKWKINPIVI
                     IIISGILGIFFYL"
     gene            complement(43373..44206)
                     /locus_tag="ACOIAF_000307"
     CDS             complement(43373..44206)
                     /locus_tag="ACOIAF_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGZ2667342.1"
                     /translation="MSWKHIKIPNGDTMIFCNSFITRKSSMNIVVTHTPIVTTLEMQK
                     AYEPLAKHNVNIFAFDFSGTGKSDGYEKDFSRNSIVKDLDSVITYIEKNYSSNIHLYG
                     NTGIGGMFAQYYACSDNKLKSFAQFACVDYKNTTGVGYTYLTVKLLNFFFKFLPNFHI
                     TVKPPKYKGYNEVYDNEFYKIFEKKNPHIWKNSTKIINTMLECFIAKDSKIKDGVNIP
                     TLVFKTLHDRYFKTQYFDSYYNSLKCKKKLVEINNVHNSYYIDSEKFCQEAYDWFVEN
                     Q"
     gene            complement(44391..47819)
                     /gene="uvrA"
                     /locus_tag="ACOIAF_000308"
     CDS             complement(44391..47819)
                     /gene="uvrA"
                     /locus_tag="ACOIAF_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HGZ2667343.1"
                     /translation="MKINNYKNQSIITNPKKFENKYQDLPKTPIELLKVVQSLVIHGD
                     QGKLYGISFNKQQSDEELLRTIPQMLKRIFEINSNPLTIPRNPKQRLVGMCRDYSLLL
                     VSLLRYRGFEARMRAGFANYFESELTYEDHWLVEYYDTLTKRWIRIDAQIDDIQKNYF
                     QINFDTHDVGKTDGFLTGSEAWIRCRQGHAHPDDFGYNKNWKGWHSVKGNLLHDFNNM
                     IGLELLPWDLWTELSSKKYNQLTRAEKNLLDEMAEILSIGNIKIEDLNLLIEKLPEDY
                     LKSIFSQLKILGISEIKELGNPLELEKKFKFTKSINKSIKNSLCHNKSSIYLKGGRQN
                     NLKDVEVTIPKNQITVITGVSGSGKSSLAFDTIYEEGKRRYFENLSNGAKLSEQLQKP
                     EFDLLQGLTPTIAIEQKKGSQNPRSTVGTLTSIWDYLRMLFVSIGKSYCPYCKIPLEK
                     KNNTKNYCPHCQTIFSKINTSTFNANSHTGACHDCNGLGFTYQVNPQLIVKDPTISIL
                     DGATYYFGKLRGKKPNGNWMVGELYAIAKDKNIDLDIPWNELPRDFIDAILYGTDDKI
                     YEFSFESKGRESKIRRPASGAINHIQRLFRESSSENNTLHQYMNKIPCNTCGGELLCI
                     EARFTTIKGYRFPELTKMTIEQLWNWLCELPNQLQKNELSLVNDILTELKIRVSYLLK
                     VGLSYISTDRTAPTLSGGELQRVRLSSQLGSELVGLTYILDEPSIGLHPRDHNLIIKM
                     IEELRDKGNTVIVVEHDKDTILSADYIIDVGPSAGTKGGFIIAEGTTQEIIKNPNSIT
                     GKYLSTYNKTGSQNKIIPSKWLSLKGCHANNLKNIDVEIPLNCMCSITGVSGSGKSSL
                     VFHSLLPALEEKLKQKSIPDKNYTEFTGFDAIDDFILMDQTPIGKSSRSTPATYINIF
                     DEIRSLFAETPQAKQKLLDESYFSFNSKKGQCPNCQGLGKTKIILQYMADQWVTCSKC
                     QGKRYQKEILSIQYKGKTIADILDMEVAEAKTFFSDCSDIYRKLSLLDEVGLGYLKLG
                     QNTLGLSGGESQRIKLAKELGTKTKKRMLYILDEPTTGLHFKDIENLLITFRKLVNEQ
                     HSLLIIEHNTEVIRASDWIIDIGPDSGINGGEIVASGTPDEIKINPNSITGHFI"
     gene            48138..50372
                     /locus_tag="ACOIAF_000309"
     CDS             48138..50372
                     /locus_tag="ACOIAF_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HGZ2667344.1"
                     /translation="MKLSRYKKNSLVLSIILCSLIISILVITVFNTVKLHNISNKQSK
                     NYLNDVSTQIVMNVDSKIKFILSDLRIMADFIKQYEGDSRYEYLSKRKTSYKYYDVGI
                     IDLEGKAEFLSGKKFDLKNTVSYKKGIEGKEYSEVIESMGFVLYSVPIFNDNHEVSSI
                     LIGVSNKESMNEILNIDNFNGEGTIEIINSKGKPLFIGKNTELIKDLSHKYKDSTDEP
                     WAQKMLNDFKNDKSGNITITSSKGVQCLLTYHPITRGLNDWHFLLIVPEDAVLGELNK
                     LNSFTITMTFIITCIIGIITLILYIIRRKYVNQIENIAYLDSITNGINSTKFSMLVKP
                     LISESPDSTYMMIAMNIKDFKLINDCFGSEKGNMTLKHLYNILYKNIDENEEFVCRHD
                     ADLFYLLIKNRPVLEALELLYKIEADTNYFNKNRENPYFLRLSVGIYTIENHNEDLIT
                     IQDHANTARKSFNKTHQSDFSFYSDIERKRLISEKEISNLMEKALENKEFFMCLQPKI
                     DIKSGKISGAESLVRWKNSEKGIIYPSDFIPLFEKSGFICKLDLYILEETCKLISKWI
                     KEGKEVLRISVNVSRQHLNDKLFLEKYKHICNKYNVPTCLIDLELTESIFLENPEAID
                     IIEDIHSNGFKCSIDDFGFGYSSLGILKDFKVDIIKLDRSFFVSKNNIDRGKVVIKSI
                     IELSKRLGMKVTAEGIEELEQVEFLKNVGCDYIQGYVFSKPLLISDFENFAYENNKIK
                     NIHY"
     gene            50628..52658
                     /locus_tag="ACOIAF_000310"
     CDS             50628..52658
                     /locus_tag="ACOIAF_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HGZ2667345.1"
                     /translation="MISGRMLLIMNFLKGKNKTSYKDISKQLSIEERKVRYDINNLNI
                     VLRSLNKSVIQKMNKGVLIIPDDFQVVCINNNEYVFSLTERVSIMKIISMFKIDNLNL
                     EKLSKEFQVSRSSIKNDLNVLSSELERNQITITYDKSFKISGEEDIIYKQRLNILKSY
                     SYLFGKEKSDLSVFEKYLVNVIEKIFKGIYLIDIYNWSMNLLLQMGWTLNDESFTWYV
                     SNIFLFTWYIHSDEKHPLESASLSEPCFENKFDQEFETIIQKKLTKEQIFLLKSFVFF
                     TNKYASLNEDMDLISTEIIVQDLIYNMSEILEISFKEDMILYKGLLNHIAPLIERIKQ
                     NIQIYEELLHVIPDKYQYVLEATKVSIQKNRVLSQIKNENETILLAIHFLGSIHRNER
                     NNYKNILLVCGFGYGVIAMLKDSLVNDYQINIVESIPSYKLQDYKEWESIDFIITTSK
                     IVIDLPKTIIEINPFLQEEDYLKFEEKGIKRKNVLTNYMSIKKRLDFLEEDAQKRVLS
                     IICQELGYSDERILFRQLNLSDLIGIDTIRIIDGDMKWEDAVKISSNILANCDFVSAQ
                     YAENIIDILKNVGFYAVKDNEFALLHGNDSSLVKVSSISLLICKEAVSFGDKKVKIIF
                     CLASRDKKEQIPAIINLTKMVYKTNFIAMLESAKTKEEAEVLIHKYEKEVTV"
     gene            52655..53107
                     /locus_tag="ACOIAF_000311"
     CDS             52655..53107
                     /locus_tag="ACOIAF_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGZ2667346.1"
                     /translation="MNKLVNVKCSFKIHVNSKEEAIISLVDVVSKEGYLIDKNQFLKD
                     VLKREETLSTYIGHGIGLPHSQSVGVKNSCITIGKLDTPIEWTEEGEKVDLIFLISVT
                     KDNENNLHLKILSKLARLLMHESFRNQIRCSDEQTVYNLIKEKIEEED"
     gene            53109..54164
                     /locus_tag="ACOIAF_000312"
     CDS             53109..54164
                     /locus_tag="ACOIAF_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HGZ2667347.1"
                     /translation="MRILKFLQKHLMTATSYMIPFVVAGGILFALSVTLSGQAAVPET
                     GWLAKLNQIGAAGLALFIPILGGYIAFSMADKPGLAPGMIGAYLAKEVNAGFIGGIIA
                     GFIAGFVVLQLKKIKLAPTMRTLGSIFIYPLIGTLITGGIIVFLIGEPIASFMTWMTN
                     WLNGMSGVSKIPLGGILGGMIASDMGGPINKVAATFAQTQVDTLPYLMGGVGVAICIP
                     PIGLGLATLLFPKKFSKEERDSGKASLLMGCVGITEGAIPFATSDPVRVIPCIMAGSI
                     IGNIMAFLLGCLNHAPWGGLIVLPVVDNRLGYIASVLTGAVVVAVLMKLVKKDVKEDE
                     EIEEDLDESIELIFEEL"
     gene            54231..54539
                     /locus_tag="ACOIAF_000313"
     CDS             54231..54539
                     /locus_tag="ACOIAF_000313"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HGZ2667348.1"
                     /translation="MKIVAVTSCPSGVAHTYMSAESLELSAKKFGIEIKVETQGSSGI
                     DNELSLKDIDEATCVILTNDVEIRNMERFKGKKVVRMSVSEIIKKSDALIKKIKDTFQ
                     "
     gene            54643..55908
                     /locus_tag="ACOIAF_000314"
     CDS             54643..55908
                     /locus_tag="ACOIAF_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase,
                     non-catalytic subunit"
                     /protein_id="HGZ2667349.1"
                     /translation="MKKLPIKTVVQRLLNLQEEGKSATLLGIGPMSPNLLQASFELAK
                     DDDFPLMFIASRNQVDADELGGGYVNGWNQEIFTKDIKKVADKVGFDGLYYLCRDHGG
                     PWQRDKERNDHLPVDEAMELGKKSYLADIEAGFDLLMIDPTKDPFEIGKVIPLDVVLE
                     RTVELIEYCENERKRLNLPDIGYEVGTEETNGGLTSTDTYETFITRLKVELDNRGLPM
                     PTFIVGQTGTLTRKTEQVGTFNFRNAYDLAQMAKKYGVGLKEHNGDYLDDVTLLEHIP
                     SQIIATNVAPQYGTEETRAYLKLAEVECKLEKEGLVEKTSNIRHVLLVNAIECGRWRK
                     WVVGEQKNLTTEEIFKDEVLSNEILDIAGHYTFNNDDVKKEIEVLYDNLSKNNIDGQR
                     FVVDHIKRPLRDYAECYNLKGVTTRILNK"
     gene            55996..56712
                     /locus_tag="ACOIAF_000315"
     CDS             55996..56712
                     /locus_tag="ACOIAF_000315"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HGZ2667350.1"
                     /translation="MINLIFDVDDTLYNQLTPFYTAYNKVFSSIKDISIEDLYMSSRK
                     YSDEVFHMTENGEMPIKEMHIYRIMKAFEELGNSITEKDAQSFQDEYIYQQSQITLIP
                     EVERILNFSKERNINLGIITNGPSNHQRMKLKQLNIENWVDKSNIFISSEVGFSKPDT
                     NIFRVAENVMNLDRENTYYVGDSYRNDVLGAKKAGWKSIWLNHRGHEVEELFYKPDFV
                     ILEHKDLISLFIKICSYKNM"
     gene            complement(56907..57200)
                     /locus_tag="ACOIAF_000316"
     CDS             complement(56907..57200)
                     /locus_tag="ACOIAF_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HGZ2667351.1"
                     /translation="MRNMIENLIKNNTYIDDKVIMLSMLSSAKASANMYLNSALTSST
                     PELRAIYSASLTQMVEGHTALTELSINKGWVKPYDKPIQQLTCSYKESQNVID"
     gene            complement(57206..57421)
                     /locus_tag="ACOIAF_000317"
     CDS             complement(57206..57421)
                     /locus_tag="ACOIAF_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667352.1"
                     /translation="MRILSETEKISLAAIIKMESDGLLMQRAINMLISDEDLKRQSES
                     SILATEGRIKAIQQFIVENEILISEEV"
     gene            57869..58267
                     /gene="tnpA"
                     /locus_tag="ACOIAF_000318"
     CDS             57869..58267
                     /gene="tnpA"
                     /locus_tag="ACOIAF_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HGZ2667353.1"
                     /translation="MYKSNKNIVYDCKYHIVFCPKYRKPVLVGEVEKMLKEILPYKAD
                     ELGAEIIEMETDKEHVHLLISCDPQYGIHKVVKGLKGFSSRVLRENFPHLKSSMPSMW
                     TNSYFVGTVGSVSLEVVKQYIENQQVRTKK"
     gene            58264..59427
                     /locus_tag="ACOIAF_000319"
     CDS             58264..59427
                     /locus_tag="ACOIAF_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HGZ2667354.1"
                     /translation="MIKTHRVKLNLTRTQFELVREKQMESANCWNYIVNLSKEYYFEH
                     KQWIKKNDIQKLIKGKYNLHSQTIQAISDKFDANRKTISELRKKGNTKAKYPYKTKKF
                     YIIPFKASAINRNAKGNLKLSMSKGRYLELDFNVENIKTAEIVWRNGYYLYYTFDNEL
                     NGVVAKGTNTVGVDLGEIHSIASVTNKGVGLILSNREGRSIKQFRNKMYAYISKRLSK
                     CKKGSRQSKKLWRLKNKIRSKTDNQLMNLYHQTTRKFIDFCVEQKVCEIVLGDIKGVE
                     KDTKKKKRLNRVNRQKISQMEYGRIKDYIKYKAKEQGIEVKLVKENYTSQTCPKCSKK
                     HKPTGRTYSCTCGYETHRDIVGAWNILNKKHKYGLVDFRINHKQPINLKVSTV"
     gene            complement(59811..60323)
                     /locus_tag="ACOIAF_000320"
     CDS             complement(59811..60323)
                     /locus_tag="ACOIAF_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HGZ2667355.1"
                     /translation="MLEVIKNRRSIRTYIDKNIEEDKITEILKSAMQAPSSKNAQPWE
                     FIIVDDKELLKELSKSQHRAKHIEFAPLCIVVLGNRDKFLKPGKWIQDLGACTQNLLL
                     EVTNQGLAACWAGVFPKNKVVNKVRQTLDLPLKLVPYALISIGYSEEKNEFIDRFDEN
                     KIHRNVYKNR"
     gene            60773..61249
                     /gene="purE"
                     /locus_tag="ACOIAF_000321"
     CDS             60773..61249
                     /gene="purE"
                     /locus_tag="ACOIAF_000321"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HGZ2667356.1"
                     /translation="MKVAVVMGSKSDYPKLEEGIKLLEKYGIEVVARALSAHRTPEQL
                     SIFLKEIEDDTDVIIAAAGKAAHLPGVIASQTLIPVIGLPIKSSTMDGLDSLLSIVQM
                     PKGIPVATVTIDLGLNAALLALQIMTLKYPKLKEDLKSYREEMAQKVLEDDKNLRG"
     gene            61255..61953
                     /gene="purC"
                     /locus_tag="ACOIAF_000322"
     CDS             61255..61953
                     /gene="purC"
                     /locus_tag="ACOIAF_000322"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006772094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HGZ2667357.1"
                     /translation="MLLYEGKAKQVYSTDNENEYVVYYKDDATAFNGEKKAEISSKGI
                     LNNKISTIIFEMLKENNINTHFIKSLSDREMLVKKVEILPLEVIVRNIAAGSICKRVG
                     LEEGVVFDEPIFEISYKNDAYGDPMLNDDYAVAMKLATREELKFLREETLKINELLKA
                     FFLKLNLKLVDFKIEFGKDSEGNIILADEVSPDTCRLWDVNTNEKLDKDRFRKDLGDL
                     VEGYTEVLSRMNNK"
     gene            61965..63332
                     /gene="purF"
                     /locus_tag="ACOIAF_000323"
     CDS             61965..63332
                     /gene="purF"
                     /locus_tag="ACOIAF_000323"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HGZ2667358.1"
                     /translation="MCGVLGIYSNKDVTKELYYSLYSMQHRGQESCGLALLDDGEIKY
                     KKDMGLVGDAFKENELSKLKGNIGIGHVRYSTAGGSHVSNCQPLVGSCRKRQLAIAHN
                     GNLVNANYLKDMLEEDGYMFQTNSDTEVILYILARYYKGDIVESLKVTMDYIKGAYAL
                     VIMSQEELVAVRDPHGFRPLVLGKKGDEYIFASENCAIDILGGEVIRDVEPGEIIVVK
                     DGELKSYFYSENYKPVKKSCIFEHIYFARNDATIDNVNAYEFRIKCGERLAQNETVKA
                     DMVVPVPDSGWPGAIGYANASGLKISEGLVKNRYVGRTFIKPTQEEREIAVKIKLNPL
                     STIIKGKSIILVDDSIVRGTTSKQLVKSLREAGAKEIHLRITSPPVAYSCYYGIDTPN
                     RSKLIASSNNVEEMREYIGCDSLKFLDIEGMLDATEHKSTFCKACFDGEYPVKKIDKE
                     ELLSC"
     gene            63326..64402
                     /gene="purM"
                     /locus_tag="ACOIAF_000324"
     CDS             63326..64402
                     /gene="purM"
                     /locus_tag="ACOIAF_000324"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HGZ2667359.1"
                     /translation="MLTYKESGVDIDEGNRAVDLIKGKIKGTYDGNVVGDLGNFSGLY
                     SLKDFVGMKEPVLLASTDGVGTKLKIAQMMDKHDTVGIDLVAMCVNDLICQGAKPLFF
                     LDYIALGKLVPEHIEKIVGGIADGCKMSGCALIGGETAEMPGMYGENDYDLAGFSVGI
                     ADKEKIVSGNNVKSGDVLVGISSSGVHSNGFSFIRKIFLETYNYKMEQYVEELGMTVG
                     EALLTPTKIYVKLALDVLAKHDIKAIAHITGGGLIENITRVIPKGLGLDINKKSWEKP
                     PIFKMIEGFNAVDERELHKSFNMGIGLVLIVDKENADDVVNFINNRENDNADYVDKKY
                     SELLEDKAYIIGEVVDSHEGVELC"
     gene            64396..64989
                     /gene="purN"
                     /locus_tag="ACOIAF_000325"
     CDS             64396..64989
                     /gene="purN"
                     /locus_tag="ACOIAF_000325"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HGZ2667360.1"
                     /translation="MLNIGVLISGGGTNLQAVIDGTESGEIKGQVKVVISSKQGAYGL
                     ERAKNHNIKAICETDEDKIIEILKENKIDLVVLAGYLKIISPKLVNEFRNKMINIHPS
                     LIPSFCGAGFYGEKVHQGVIDYGAKVTGATVHFVDEGADTGPIIMQDVVKVNQDDDAK
                     TLAKRVLEVEHRILKESISLFCENKIKLQGRRVFINE"
     gene            64982..66514
                     /gene="purH"
                     /locus_tag="ACOIAF_000326"
     CDS             64982..66514
                     /gene="purH"
                     /locus_tag="ACOIAF_000326"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HGZ2667361.1"
                     /translation="MSKRALISVTDKTGVVEFAKELNKLDYEIISTGNTFKTLKENGV
                     NVMQVEDVTNFPEILDGRVKTLNPYIHGGILYKRDKESHVETVNEHKIHSIDLVAVNL
                     YDFEGTLKAGKSHDEIIENIDIGGPSMIRSAAKNYKDVIVVVDIKDYDSIIEKLKTDT
                     MTLEDRKKLSYKAFSTTGRYDALISSYFAGEVGDTYPDILNLTFQKEQTLRYGENPHQ
                     NGFLYSQSNAKNPILNYEQLGGKELSFNNLNDLHGCLEVMREFKDSEEVVSVAIKHAN
                     SCGVGLGKDAFEAYTKCYEADKVSIFGGIVGITSTIDKATAEKLNEIFLEIVVAYDFE
                     PEALEILKQKKNLRILKLAKIENSLQPYEMKYLDGKLLIQDRNNILAEKSENVTKEKP
                     TDAQLKDMEFGMRVVKNMKSNAIAIVKNGQTLALGCGQTSRIWALKNALENNKDKDFT
                     GAVLASDAFFPFDDCVTLAHEYGISAVVQPGGSIKDKDSIEACDKYDMVMVFTGIRHF
                     KH"
     gene            66525..67775
                     /gene="purD"
                     /locus_tag="ACOIAF_000327"
     CDS             66525..67775
                     /gene="purD"
                     /locus_tag="ACOIAF_000327"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HGZ2667362.1"
                     /translation="MKILVVGGGGREHAICWKLSKEKNVEKIYCAPGNAGIANVAECV
                     NIGDTNIEELLKFAKENEIGLTIVGPEVPLVMGIVDEFEKEGLRVFGPNKKCAQLEGS
                     KAFSKEFMIKHNIPTAKYKEYTNLEEAISEIDSFGYPVVIKADGLAAGKGVVIPENRE
                     DAIATLKEMMSDKKFGAAGDKIVIEEFLKGIETSILAFVDNDTIVPMASAKDHKKVNN
                     YEQGPNTGGMGTFSPSEIYTEELANKVKETVLEKTLEGFKKDGLNFKGILFVGLMITE
                     DGEKVLEYNVRFGDPETQSVLFRLETDLHEIMEAILDNKLKDIEINYSDDEAVCVMLT
                     SGGYPDSYEKGKIITGLENLDDDIVVFHSGTKMFDGNLVTNGGRVIGITAKSTTVKDA
                     AEKVYENIKKINFEGMHYRTDIGR"
     gene            67800..71606
                     /locus_tag="ACOIAF_000328"
     CDS             67800..71606
                     /locus_tag="ACOIAF_000328"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HGZ2667363.1"
                     /translation="MLNTENKDSMVRRVLVEKREGFDLEAKALKKDLVESLHIDNIEN
                     LRILNRYDVEGISEEVYENAAKTIFSEPNLDVVYYEEIPKLNDERVFAIEFLPGQYDQ
                     RGDWAAQCVQIVNQGIRPAINTAKVYILSGKITDEEFSKIKDYCINPVDSREASLEKP
                     ETLKMETEIPTTVEVLDGFIDLDENGLKTFVSEKGLAMTLGDLQHVQKYFKDTEKRNP
                     TITEIKVLDTYWSDHCRHTTFMTEIENVKIEDGKFNDIVKEAYQMYLNSRDNVYVNRH
                     KDICLMDIATVAVKELKKNGKLNDLDESEEINACSINVDVEVDGKMEKYLVMFKNETH
                     NHPTEIEPFGGAATCLGGAIRDPLSGRSYVYQAMRVTGSADPRTTLEDTLPGKLMQRK
                     ITTEAAHGYSSYGNQIGLTTGQVAEVYDENFVAKRMEIGAVIAAAPKENVVRERPEAG
                     DVIVLLGGKTGRDGCGGATGSSKEHSEESILTCSAEVQKGDAPNERKIQRFFRNKEVA
                     QMIKRCNDFGAGGVCVAIGEIADSLDINLDLVPKKYDGLDGTELAISESQERMAVAIK
                     KENKDKFIQLAVEENLEATHVATVTDTGYLRMFWNGKAIVDINREFLDTNGVKQTTDV
                     HVTKVDEENTFFSSNEIVKDVKCASMKDKFTKVLSDLNVCSQKGLVEMFDNTIGGNTV
                     LMPFGGKYQATPTQGMVAKIPVLGGETNTSTIMTYGYNPKVGKWSPFHGALYAVVESV
                     CKLVAIGGNYSTTRLTFQEYFEKLGNNPEKWGKPFSALLGAFYAQSKFEIPAIGGKDS
                     MSGTFKDIEVPPTLVSFAVDTVDAKKVVSPEFKKADSKVVMLCVNKAENDVVDFEELK
                     RNLDKVRELIHGNKVLSTYALGFAGVGEAISKMAFGNKIGFKFSEEAEKAFTDDKLFE
                     ASYGNIVLELANDDLSMLEGYNYVVLGSTVKEASIFIKGEELALDELYKAHCSTLEPI
                     FPTKTEEVKSKIETISYISQGEAKKSSLSIATPRVFIPAFPGTNCEYDSARAFERAGA
                     NASIRVFKNLTYKDIEDSIDTIVNEIKSSQIIMLPGGFSAGDEPDGSGKFIATVFRNP
                     RVQEAINEFLTQKDGLMLGICNGFQVLIKLGLVPYGEIRVPSESAPTLTYNNIGRHQA
                     KIARTRISSNKSPWLAQTNVGDIHNIAISHGEGKFVASEDVMRELIANGQVATQYVDF
                     NNEATYDIEFNPNGSFYAVEGITSADGRVFGKMGHSERIGEEVYKNIIGEKDQKIFES
                     GVKYFR"
     gene            71812..72684
                     /gene="rfbD"
                     /locus_tag="ACOIAF_000329"
     CDS             71812..72684
                     /gene="rfbD"
                     /locus_tag="ACOIAF_000329"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HGZ2667364.1"
                     /translation="MKILITGSNGQLGKELVNQLEAINQSINQPKYVILATTRSDLDI
                     SNQTNVDNFILHNKPDVVVNCAAYTKVDACEDNIEIAYKINALGVRNLAIASEKVNAK
                     LIHISTDYVFNGFSKYPYREDNKTEPNSVYGKSKLMGEKFVEQFSHKYFILRTAWLYG
                     NGNNFVKTMIKLSLENTEVNVVNDQFGTPTSTVDLAKIIIKIMETEHYGVYHATCEGE
                     CSWYDFAKKIFKLKNIDIKVNPIKSNEFKCKAPRPLYSVLDNFMLKLIGLNSFRKWEE
                     SIEEYLERYDINEK"
     gene            72793..73662
                     /gene="rfbA"
                     /locus_tag="ACOIAF_000330"
     CDS             72793..73662
                     /gene="rfbA"
                     /locus_tag="ACOIAF_000330"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HGZ2667365.1"
                     /translation="MKGIILAGGSGTRLYPITKCISKQTLPIYDKPMIYYPMSVLMLA
                     GIQDILIISTPRDIKAFEELFGDGSSLGLNITYAIQEKANGIAEAFIIGEEFIGNDRV
                     SLVLGDNIFYGYGFSERLENAVKRKGATIFGYHVSNPNSFGVVEFDKDFNVLSIEEKP
                     TVPKSNYAIPGLYFYDNDVVEIAKLVEPSDRGELEITSINSEYLKRGKLKVELLGRGM
                     AWLDTGTHKGLLDASNYVEVVQTRQGLYIACLEEIAYTKGYISREQLMKLAKPLIKTE
                     YGQYLLKLASEDM"
     gene            73711..74268
                     /gene="rfbC"
                     /locus_tag="ACOIAF_000331"
     CDS             73711..74268
                     /gene="rfbC"
                     /locus_tag="ACOIAF_000331"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HGZ2667366.1"
                     /translation="MGKINVIKTKIEGLYIVEPNVFYDERGYFMESYNKKDFIEQGLD
                     MCFIQDNESSSRKGVLRGMHFQVKYPQGKLVRVTEGEVFDVAVDLRKDSQTFGKWEGV
                     ILSDKNKKQFYIPEGFAHGFLVLSEKAVFSYKCTNYYYPEFENGFIWNDKQISIEWPL
                     KGIEEIFLSEKDKKQKSFKDICTRR"
     gene            74306..75289
                     /gene="rfbB"
                     /locus_tag="ACOIAF_000332"
     CDS             74306..75289
                     /gene="rfbB"
                     /locus_tag="ACOIAF_000332"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HGZ2667367.1"
                     /translation="MKKILITGGAGFIGSNFIHYMLNKYVDYFIVNLDLLTYAGNLET
                     LIEIQDNSNYKFVKGDISNREQVYKLFEEERFDIVVNFAAESHVDRSVENPDLFIKTN
                     IIGTQVLLDASIKYGVKRYHQISTDEVYGDLPIDRKDLFFTEQSSINPSSPYSASKAS
                     ADLLVSSYYRTYGLLTTISRCSNNYGPYHFPEKLIPLMILNALENKQLPVYGNGENVR
                     DWLHVYDHCTAIDLIIHKGNIGEIYNIGGHNERSNLEVVKMILNLLGKSEELISYVND
                     RPGHDLRYAIDATKIENELGWKAKYDFDLGIKETVKWYIENESWWKAVLSV"
     gene            75319..75987
                     /locus_tag="ACOIAF_000333"
     CDS             75319..75987
                     /locus_tag="ACOIAF_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HGZ2667368.1"
                     /translation="MNEISSLINILALTQLSGSNTTNQCNCTYNNSSGFDMIMMTLLK
                     ALSQNNQHTSNSYNLLSNSENLFNELDNVVNNTTSRFIDVDTKDKNVKLRIENAVDQA
                     SKKYNVDSNLIKAIIKVESDFNPNTVSSAGAKGLMQLMPENCRDLGVTDPFNIEQNID
                     AGTRHIKEYIDMFGGSIEMGLMAYNGGPGRMKSRGVESISDLYKMPKETQNYIPKVMK
                     YYRG"
     gene            75996..76424
                     /locus_tag="ACOIAF_000334"
     CDS             75996..76424
                     /locus_tag="ACOIAF_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaR family protein"
                     /protein_id="HGZ2667369.1"
                     /translation="MGINIDKLNISPSINMEVKKLKESNKFSDKLNSLKQSSSLEDIK
                     RNLESVKKAGERLVLTQNYGDISIYKNAVKEYLKSVVDNMYSLDKNSSFWEHQYYKNV
                     KIVDSKLEDMTSKLISEEKENIDIVSTVDMIQGLLIDMYR"
     gene            76448..76762
                     /locus_tag="ACOIAF_000335"
     CDS             76448..76762
                     /locus_tag="ACOIAF_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HGZ2667370.1"
                     /translation="MNSKKEDIYELNFGQLIEEKSGVIEENQNVFNAKVNVAVSIGNT
                     TETIKNILDLKEGSIIKLSKNVDEKLDIYANDRLFAYGESIITNERLSVRLSKIEDPD
                     VN"
     gene            76914..77189
                     /gene="flgM"
                     /locus_tag="ACOIAF_000336"
     CDS             76914..77189
                     /gene="flgM"
                     /locus_tag="ACOIAF_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HGZ2667371.1"
                     /translation="MDIKNVSSNIVVNNYLKNTSGIKKVEEVKKINKTDKIELSKFAK
                     EMASVSNMSDENIRNKKVDEIKNALENGTYKIKPKEIAKKMIEDMRG"
     gene            77194..77610
                     /locus_tag="ACOIAF_000337"
     CDS             77194..77610
                     /locus_tag="ACOIAF_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HGZ2667372.1"
                     /translation="MITDLKVIIYEEKNIISKMMELLKEQFDYIVEKDLENLNMLNPK
                     LEDISRELASIEIKRRQLFGDDISMSEVVENSNDGYLKEIYTDLKHILALAKNQQESN
                     DSLIKKELIFTKKMINFIKPADKKTTTYNSYGNIRK"
     gene            77628..78938
                     /gene="flgK"
                     /locus_tag="ACOIAF_000338"
     CDS             77628..78938
                     /gene="flgK"
                     /locus_tag="ACOIAF_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HGZ2667373.1"
                     /translation="MAGLFSILNTANSGMNAHQKAIQTISHNISNLDTDGYSRQRTEF
                     ATNSPMYMPSLSNSIARGQLGTGVHVTDVTRARNSFYDYQFRAEAHKYGKTVSKYDYY
                     NTIETILNEPSDYGISAGIDDFFKGWESLSKDINSDSKKSLVVENASNLATLISESYK
                     KLDKLKEDIDSNIDTEIKQINDMLKSLEDLNKSIDIISGSGSTPNDLLDERDRILDNL
                     SFKLDFENSDVKNMLADGKLELNELKNPDGTWKTGISGTLQGLFEMHGKIDTYKSDLK
                     DVSDGLAKQINDVYNTSAGITVTDFFITSNVDGEDIIKINPAIKSNPNELKLTADEAS
                     KIAKLKDEKIDIGVAGGKVSTISDHYKAFAESIGLDSQKVNQDEANQRKIINNIDNSR
                     MSVSGVSLDEEMTELIKIQRSYQASAKVMSTAVQLLDVVINGII"
     gene            78957..79889
                     /gene="flgL"
                     /locus_tag="ACOIAF_000339"
     CDS             78957..79889
                     /gene="flgL"
                     /locus_tag="ACOIAF_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HGZ2667374.1"
                     /translation="MRVSTGMMSSSYLNSLQDNLQRLDKVNRQVNTTKEINKLSDNPY
                     KAIKILNSKSEIKTMETYVENCKDTGNWLETTDTALDQLGKLLSDIKKTLVASGDGAY
                     SDDELKTLSNETNEKMKEIANALNATHEGKYIFSGSNTGVPPVECIDNADGSVSLKFN
                     SSLNLNKLNDSLSMDIAQGISVDYNVKLSSLGFDPTKTDPFEELNNISKKLANPDADA
                     IKELTTTCLGDMEQLIENTVNVRSIYGTKANTVEAMKEKNDDSLIQLKDILSQNEEID
                     YGEKLVQLKAAELTYQASLQTGGKLFNVSILDYI"
     gene            79905..80297
                     /gene="fliW"
                     /locus_tag="ACOIAF_000340"
     CDS             79905..80297
                     /gene="fliW"
                     /locus_tag="ACOIAF_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HGZ2667375.1"
                     /translation="MMKVTLKKGILGFENLKEYELLDIEDNDYLKEFNSTEEEGIGFV
                     VVSPFEIINEYEIVLNQETIEKLEVKSPKDVVLFNIITLGQTFEESTVNMKAPIVINI
                     KTNFGMQIILQDDKYSIRHPLLRGDNGC"
     gene            80291..80503
                     /gene="csrA"
                     /locus_tag="ACOIAF_000341"
     CDS             80291..80503
                     /gene="csrA"
                     /locus_tag="ACOIAF_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HGZ2667376.1"
                     /translation="MLVISRKKDEAVLIGESIEVKIVGVDGNNVKLAISAPNNISILR
                     KEIYEKVKSENIKATNKNIKILKSLK"
     gene            80525..80920
                     /gene="fliS"
                     /locus_tag="ACOIAF_000342"
     CDS             80525..80920
                     /gene="fliS"
                     /locus_tag="ACOIAF_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HGZ2667377.1"
                     /translation="MYGENPYNSYKQNAVFMASKEQLLLMLVDGAVKYTKIAKTAILD
                     KNTQKAHRELIRVQDIFTELMVTLDQNAGQWAKDMYRVYDFVRYELSRANIRKDIQII
                     DNVLPVIEQIKDTWHEADKKSKEERSRYK"
     gene            80920..81276
                     /gene="fliS"
                     /locus_tag="ACOIAF_000343"
     CDS             80920..81276
                     /gene="fliS"
                     /locus_tag="ACOIAF_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HGZ2667378.1"
                     /translation="MELNLAELDKLSKEELLLMLVDGTVKYTNISKEALLNNDYLKAH
                     NELVRVQNIFTELMTTLDQDAGQWAKDMYKVYEFIKSELAIADENKDIKIIDDILPIV
                     KQIRDTWHEVYNQLKI"
     gene            81297..82820
                     /gene="fliD"
                     /locus_tag="ACOIAF_000344"
     CDS             81297..82820
                     /gene="fliD"
                     /locus_tag="ACOIAF_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HGZ2667379.1"
                     /translation="MSSISPIRVTGLSGNFDMEGIIEASMIRDKEKVDKAKQEQQIVK
                     WKQEIYRNVIQESKDLYDKYLSVNSPNSIVSEKAYSSTRITSSDESIIVAKGSAGAEK
                     INYQFAVSQMAEPAKFTIKLNSSEPIVQQFPPNASGASSLTIGDVNIPISEQDTTSTI
                     VSKINSLCADNDIKASYSEMTGELIISRKQTGSSSDINLRVIGNDNLAQQIANDNGIT
                     FINDAGGNKVANVYGKNLEADVTDEHGRVTHISKEQNSFNIDNIDYNVNSKGTAKLTS
                     VTDTEEAVKNMQAFVDDYNKLMDKVYGLVTTKKPKDYPPLTDAQKEDMTTEEIEKWEK
                     KAKEGILRNDDELRGFVEDIQSAFFGDGKNIIALRKLGINESENYNKKGQISFNADTF
                     SKALIDDSDKVYKTLAGYSSNYDDKGMFEKLKDIVYEYSGSSTSKLPKKAGIEKTASA
                     SENVYSKQIAEQERNISRLVEKMNDKEKRLYAKYSALESLLNQYSSQMNYFSQAQGN"
     gene            82823..83158
                     /locus_tag="ACOIAF_000345"
     CDS             82823..83158
                     /locus_tag="ACOIAF_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HGZ2667380.1"
                     /translation="MDKLKEKLNLYKDISLQIINLIEKEEYIKISIKLGERQEIINSV
                     SEIDRNDFIQLYNRMELIEIDSRIRDILQGQLLEVKKELHEYKLTKQVNTMYYNLNRE
                     KVNIFNKKV"
     gene            83260..84132
                     /locus_tag="ACOIAF_000346"
     CDS             83260..84132
                     /locus_tag="ACOIAF_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HGZ2667381.1"
                     /translation="MRVNTNVSALIANNQMGRNVNAQSKSMEKLSSGVRIKRAADDAA
                     GLAISEKMRAQIKGLDQAGRNVQDGISVVQTAEGALEETGNILQRMRTLSVQSSNETN
                     TAEERQKIADELLQLKDEVERISSSIEFNGKKLLDGSSTEIRLQVGANFGTNVAGTSN
                     NNNEIKVALVNTSSIMSKAGITSSTIASLNADGTSGTNAAKQMVSSLDVALKELNTSR
                     AKLGAQQNRLESTQNNLNNTIENVTAAESRIRDTDVASEMVNLSKMNILVQASQSMLA
                     QANQQPQGVLQLLG"
     gene            84225..86366
                     /locus_tag="ACOIAF_000347"
     CDS             84225..86366
                     /locus_tag="ACOIAF_000347"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HGZ2667382.1"
                     /translation="MLLSIVMIVKNEEKILEKTLKSLTTLRNSIESELIIVDTGSTDD
                     TIKISKKYTEKVYFHNWNNDFSSMRNISISYAKGEWLLILDADEILIDSSTIVKFFKD
                     GLDKKFNSASIQLKNLYSYDKKLYGYCSVLRLFKNVDFRYHGKVHEQPIYKNPIFNNV
                     ADFEHYGYLFEDEEIRLNKVKRNEKLLFQELKENDNSPYTNYQLGKNFIILGKYQEAL
                     YYLEKSYELYSKLELAPGYLITSLVKTYLYLGKHKKCERLCLKYIKKDMNNIDIYYYI
                     AQAQVDLGKYENSIDSYKRYIYLLDNYEISTQANSLFSDTDTVGLRDEAIITLIKIYY
                     KLEKYEFVISEYNNIEDVEKKKNVYFSLFMSLYKLNRFEDIKNYYKEVSNSKIEKNSF
                     YRNIEEVINNIKEDEKEYVYKILCDIEGNYGILNQIRINKCVSIDKCKEILNEEKEII
                     YAYIIKIAFEEKIDLLDIFYEMEYVWIEKYLKYLLALERSFNLVLYKYIISRPNTSDM
                     EKIRVYKAISKVVLESISLSDEKYKEIFYLYIMYSYKYIRYIYSNLSDFELIKFVCND
                     LDKFTIEFKHLLDIKNKVNNANEKLEYIHSMRKLLNEYPFYNKIIKILIGELEENIKE
                     SEEFKDLKDSFIISIENMIETGKINDAKLLVDDYSKTFVDDIKILNIKGILYMFENKL
                     DKADFMLKKALSLDVENEDTIYNIEYLKSLR"
     gene            86390..88267
                     /locus_tag="ACOIAF_000348"
     CDS             86390..88267
                     /locus_tag="ACOIAF_000348"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HGZ2667383.1"
                     /translation="MNIPIVVNEKSKDIIKDWQYTCTNEDIKFTSIIILTYNQIEYTK
                     LCIESIRKFTPKNKYEIIIIDNNSSDGTVEWLKGQSDIKSVYNNKNLGFPKGCNQGIE
                     LATGENILLLNNDVIVTPNWLYNLNKALWSQDNIGAVGPITNACSYYQQIDVDYKSIE
                     DMFIFAHKINKSNINLWKYRLKLVGFCMLIKKTVLDNVGVLDELFTPGNFEDDDLSFR
                     MIESGYKLLLCQDTFIHHFGSVSFKENRNKYTDIMYINRRKFETKWGFNPEYSGMIRT
                     DLISKIDDSLEANINVLEIGCGIGATLLEIKNIYKNASIYGIEISESAGKLAKYSADV
                     LIANIEKVKLDYKKDFFDYIILGDVLEHLIDPWKIVNELKQYLKKDGYLVSSIPNIMH
                     VSVLRNLVLGNFTYEDSGILDKTHLRFFTLNEITKMFNDSNYIINSITATSMPLSEID
                     NSFIDTICKESCKEMRQQYESYQYIVKASKILDTKRYSSISMINLKYALMRIDNELDV
                     DSNLSYIFDNYNITEPCFVDDIEYLIDTFVINKATLLNRLGVEAFNRGLGDFSINMFM
                     TVLEMDRDDIDSIYNITSVLCELEEFDTAYNLIKNSSEKVQSESDIQEILCFIEGKKY
                     E"
     gene            88260..90374
                     /locus_tag="ACOIAF_000349"
     CDS             88260..90374
                     /locus_tag="ACOIAF_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HGZ2667384.1"
                     /translation="MNNDKICFITCVNDERLYEECLVYINSLDIPKNFSIETVALRGA
                     DSIASAYNKAMRETDSKYKVYLHQDTFIINKNFIHDILEIFKKNTDVGMIGVAGVKDM
                     PVNGIWWDSDKKAGKVYDSHTGKMDIYEFSNFEECFNEVMAIDGLIMITQYDVFWRED
                     KFDGWHFYDLSQSMEFRQNKYKVIVPKQNKPWCMHDSGVVNINNGYDEYRIKFVKEYI
                     NLDLYRENFYEFDKKKASTILKSNKHVMDCMKEELTEQLRLKNYKDVLGIVSKIAHFS
                     VFNHTGLYSCEECERALLECANNLPEIENTKLFTKKDLKRRKVLHVLSEGYSTGGHTR
                     LVKNWIEIDKDSVHSVVTTWQSDTLPDWLLKAINDSGGEVISLDLISDDYLKRAVALR
                     KIAYDWADIVILYVHMWDTIPVVAFGVDGKIPILHVNHYDSTFWLGTSIADIVIDFSE
                     TSQLITLDKRGIDKSEILPMPLKEKEVFSKSEVRKRYNLEDDVTIILTIASERKYNSV
                     NDINYFKIAKTLIENTDNTIIIVVGVDTENLYWKTLSNVTDNRVICFGTTANIDDFYK
                     ISDIYLESFMVGSHTSKLDAILYDLIPVKFKNEVSPIFTNMWNFIDKFDYKNIKEVID
                     MIRRYNTSEFDRDKIKAKQSEIKHFVIDKHCIEIKNYIDKIYNEVNHHKVKYDLYKND
                     EIEDYQLFVALYGYMVKNKNMS"
     gene            90383..91378
                     /locus_tag="ACOIAF_000350"
     CDS             90383..91378
                     /locus_tag="ACOIAF_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HGZ2667385.1"
                     /translation="MNNIHINKFEKSCEKNWDYFVENKSINGTFLQTRNFLNYHEEGK
                     FTDHSLIICKGEEILAVVPACETYEDGERIFFSHLGSTFGGIIIRSGFNDIRHVNAIT
                     NKLEIYLQEQNFDKVYLKNTSRIFCKEDTSLLEYFLFKNNYMFYDELSMFLELSKYKE
                     DILANFKYSKRRDFKYSLKNNLTFKRLYSDEDIVCFFNLLSNCLDSKGAKVIHSVEEL
                     LEFKNNRLKDIVEFYAVYIDDIMICGSMVFNFQERVYHTQYLASNSTYNKYYPMIFLY
                     YNLIKDALERNFKYLSFGISTLNRGKYLDEGLATFKEGFGSSAICNRTYYKNINK"
     gene            91470..92702
                     /locus_tag="ACOIAF_000351"
     CDS             91470..92702
                     /locus_tag="ACOIAF_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HGZ2667386.1"
                     /translation="MKRLMILGSIKYFENIVMSAKREGIYTIVCDNRIDTPAKKICDE
                     AIDVDVFDFDKIKNIAIDKKIDGILTGFTDSLMKPYVYIANELNLPCVISCNQLESVT
                     NKAVMKNYFKNNGIPTTDYCIIESLKDLEKIQGLNFPLVIKPVDGYGSRGVYFVSNIE
                     ELIEKFEYSKEASQDGQVIIEEFYESEEIQGLAWVHNGESHVFYIGDRELVNIHTGRA
                     GKPDRLIYPSKYCFQYEEEIKSIYQNITQAFGIKNGPLYVQMLVGAYGIKVSEVMPRL
                     PGGCDYMAISQITDLDIGSLFANFSVNNEVNFDEIKKYNMHLSKCVYALPIYIRPGTI
                     KKINNIDKIENLDYVTQFLLLVKEGDTIGLYEDERQDCGRVYGVANNVFDANIKKKCI
                     HEMIEILDENNENMIENF"
     gene            92722..93666
                     /locus_tag="ACOIAF_000352"
     CDS             92722..93666
                     /locus_tag="ACOIAF_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase"
                     /protein_id="HGZ2667387.1"
                     /translation="MKLITHEQIMSLNISQKQGYEWIKESFLTKKDCQLPPKISMVEE
                     GHVFYNVMPSLNHNHKIGGIKMVTRLPGRNPSLNSEIFLYNMEDAKLKAILDGNYITA
                     FRTAAVAALAIDTFAKKDFNTIAIMGLGVTASAIMNMLSYTLKDKNINIKLLKYKDQA
                     ESFVKKYEKNEQFSFEICDTHEELIVDSDVVISTVTYTDEYLSEFKWFKKGALLLPVH
                     VMGFGNVDYLFDKVYLDDYNHLKHLENIDRYKYSCEFYDVLSKQAKGRENDDERIISY
                     NIGLSIHDILFANKIYELCETLSCEEIELKEPKEKYWI"
     gene            93760..94167
                     /locus_tag="ACOIAF_000353"
     CDS             93760..94167
                     /locus_tag="ACOIAF_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FdtA/QdtA family cupin domain-containing
                     protein"
                     /protein_id="HGZ2667388.1"
                     /translation="MDKYNLIRFVSIGNEYIGNLVALESNKAIPFDIKRVYYIYDVPN
                     NVKRGFHAHKNLEQVLVCLNGSVKIKCYDGVKETIFNLHKNDVGLYIGTGVWHEMFDF
                     DEKTVLISIASEHYNEEDYIRDYKEFLKYLNNM"
     gene            94245..95345
                     /locus_tag="ACOIAF_000354"
     CDS             94245..95345
                     /locus_tag="ACOIAF_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012815981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HGZ2667389.1"
                     /translation="MNLLLVKFNNLLKQFQTHKEEYEQKIKDVMESGYYILGNEVKHF
                     EEEFANYVGSKYCVGLASGLDALIIAFRALGISEGDEVIVQSNTYIASVMGISINGAT
                     PVFVEPNEYYNIDSSKIEEKINSKTKAILVVHLYGQASNMKDIQELCDKYNLYLIEDC
                     AQSHGAKYDGKMTGTFGDIGCFSFYPSKNLGAFGDAGAIVTSNEEIADKVKILRNYGS
                     EKRYYNKFVGYNSRLDEIQAGLLRVKLKYLNEIEIEKSKIVDRYLNEINNPKVILPKI
                     ENNCTHVWHLFVLQVEKRDTFMEYLKKNGIESIIHYPIPPHLSEAYAYLGYKKGSFNI
                     AENYSDKVVSIPLFIGMEDEEVSYIIKIINEY"
BASE COUNT        37285 a        10489 c        16377 g        31267 t
ORIGIN      
        1 ttctctcgaa atatataatt tagaatcttc tgatttattt ttatgctcga tttccttatt
       61 ttttttcata aatatatata gaggagctat taacaacatt gcccctaatc ctattaaaac
      121 tctattttga gttataggtt ttgaaatatc ttctgcccaa gcttgttgta acaaccataa
      181 gctacctaaa atagctacta ttggtataac agggccaaat ggaatcttaa aagaggcctt
      241 aacatctgtt ctttttctta aaactattac tgataaacaa gttggtatgt actgagcaaa
      301 tcttgcaatt acgctcatta ctgcaagttg ttcaaaactt ccagttagca caagtggtat
      361 acaacaaatt aaagatatta ttattgctac atatggagca ccatatttat ttgtttttcc
      421 tataaatgca ggaagagaac cttcttctgc tagagaagaa ccacatttag gagttactat
      481 tgatgaacct atatttatac caaatataga tattaatgtt gctacagata tgaaaacttt
      541 accataattg cctacaaata cacttgaagc atctgcaatt ggaatactgt tttcaaatag
      601 tctatctcca agtataccca tacagacaat ttgaattaat atataaatta ttgcacatat
      661 aaatattgtg gttattaatg cgactggaag atttttttta ggattttcca tttctccact
      721 tgccacaata aatgattcaa agccggtaaa tgcgtaaaat actaatataa tggctggacc
      781 catactagag ttaactacat ttcctgaagg aacaatattt acaaatttta taaagaatat
      841 tccaactatt ataaaaacta ttaatggtac taatttagct atagttatta cattattcat
      901 tatctttgtg cttttaattc caaataagct atttattgaa agtagtgtta cgagtattgc
      961 agctatataa cctttgtatt cagtggcaga ttctggccaa aatgaaccta aagctgttgc
     1021 aaatcctact gctagagtag accatgatat aattctaata acccaagaca ttgtaccaac
     1081 ttcaaatcct ataaaatctc caaaagcttt tttggagtat agatatgctc ctccattctt
     1141 atcaaacatg cttccaactt ctgcaaaaca taaaagtatt gatagaacta agagtgtagc
     1201 aaaaatgtat ataaacatac tgttttgacc tgctagatta taaactttac ctggtagtaa
     1261 gaatatacct gaacctatta tgctattaat ccctaataat atcatactaa atagacctaa
     1321 tttcttagct ttttccatat taatttatct cctattcttt tattaattac tgtactaatt
     1381 aaaatacaaa gtattcatta catgcatcat aacacctatt ggtaaacaat cttcatctat
     1441 attaaagcta gaggtatgaa gaggtgaaat taagcccttt tcctcatttt tacatcctaa
     1501 atggaagaat gctcctttac aatgttctgt gtaaaaagaa aagtcttcac cacctaaaga
     1561 cgggtttggt ctcaatataa atttttcctc tccaagtaat tctttagtat ttgatataac
     1621 tgtatctact aattcttttt catttataac agcaggatag ttttcatcac ttacatgtaa
     1681 agtcccaaca caaccaaatg cagatgcagt atctccaaca attttagcta ttttatcaat
     1741 cataaagttt cttgttttgc tatttaaggt tcttaaagtg ccttctaatt ttacatcttc
     1801 acatattaca ttgtgtgcat caccaccata tattttacct atagtcaaaa cagcagcatt
     1861 atttggttct aagtttctac ttattatagt ttgtaatgat gtaacaattt gtgaagctgt
     1921 aactattgca tcaataccgt tttcaggata tgcaccatga gctcttttac ctttgatact
     1981 tatttttata gtgtctacac tagcatttaa tgtatcatat tttgtttcga taaggcctgt
     2041 ttctatatgt ggcataacat gaagtccgaa tatataatcg gccttggggt tttctaaaca
     2101 cccatcctca actaaaaatt ttgctcctcc aaaaccttct tctgctggtt gaaataagaa
     2161 ttttacattt acattaagtt tatcttttat tgaatgtaaa actttacatg ctccaagtag
     2221 catggttgta tgtgcatcat ggccacaagc atgcattttt ccagaatgta ttgatttata
     2281 aggtatgttg ttttcttctt caataggaag agcatccata tctgctctta tacaaatcgt
     2341 tttatctgca ctttctttta aaatatatgc cataattcca ttgtgttttg taaattccat
     2401 ataatcaatt ccgatttctt ttaaataaga aattaccttc tgtttagtaa gattttcttc
     2461 aagagctaat tctggaattt tatgtaattc tcttcttatg tttataagcc atggttttat
     2521 agaattacaa ctttctatta caaaatcttt tcctaattct ttggtttgat attttaattt
     2581 caaagcctgt tgcattttta ttcctccaat tgctttttat tgttttatta atataataag
     2641 caattattat gccataattt tcgatggaga attttgtttt ttataagaat attgaataaa
     2701 tataatcatt tatctatatg tatagtataa aaaatgattg catagtttca attttcagta
     2761 aaaaatatta ttttgaagaa aatatatttt atatgctagt ttatataaaa aaaataggaa
     2821 ataaaaaaat tatcctattc ctattaattc ctattattta tgtacaaaat aaattataat
     2881 tttagggtac ttgaaaaata aaaaaaccta ttgaaggagt actttaatcc tgaatagatt
     2941 tctttgtttg ataaattata attttataga cctttatatt ttaatttatt gtaaaaatct
     3001 tctccttttt gagttatttt acttccatat cttcctgaac tacaaattat aagttcttct
     3061 ttttttagat attcaaatat ttttttaatt tttccttctg taatttgaag gttcttatcg
     3121 agtagtgctt ttaaaattaa tcctctacca ataccaacat ctgattcctt atttaaaatt
     3181 agtatctcta atatattcaa tatatcattc ttactatatt ttagttttaa tgatgtattt
     3241 ttgttatctg aagaccttaa gtttggaggt aaatctttct ctaatacaat atcttcacac
     3301 ataatgttta aataggatgt aacattttgg agttctctta tattaccttt ccacatatag
     3361 ttttgtataa gtttttttgc ttctggtgtg attactaatt tcttattaat taaatcttcc
     3421 ataataatta atatgtcttc ttttctttct cttagaggag gtatatttat tggaattgta
     3481 tttaatcgat aatataaatc ctctctaaat tggctattat caatcatttg ttctaaattc
     3541 ttatttgtag cagatatgat tctaacatct atattaatta cattgtgaga tccaacaggc
     3601 attatctgct tttcttgaag aactctaagt agttttgttt gtaattctaa aggcatatcg
     3661 cctatttcat ctaagaatat tgttccatta ttagcaagtt caaataatcc ttgttttcca
     3721 tcttttagac ctccagtaaa agttcccttg tcatatccga atagctggct ttctaataaa
     3781 ttttcaggaa cggctgcaca atttactgct ataaagggtt gattttttct atttgagttg
     3841 ttatgtatag actgagctat gagttcttta ccagttccac tttcacctct gataagtatg
     3901 cttaaatcag atttagaaac cttcttagct aaatctatac attcaaacat tttaggacta
     3961 tttgttttta tatccttaaa tgtatatttt gctatttgtc ctttttctct taatttttta
     4021 gtgagatttt gttctaattt ttttatgtat gttatttctt gtaaactata atatgtgccc
     4081 attttttcat ctctattgta tatgtttttt ttatttacat ttatatattt cttattgaat
     4141 acgacaactt tatctagtat ctctttctca ctcaataaaa cttttaaact atctacaaat
     4201 atatcctcaa tgtattttcc atatatatcc tcatttatgt ctaaaatatc ttttacttta
     4261 ctattatatg tatttatttc accatctttt gaagtaaata atataccatc ttttgataaa
     4321 ttcaagattg tatctaattc ttctattttt aaaaataatt ccttatattt atcttttata
     4381 ccagtatcta aacttattat ttcttctgag tatttaacta gattagattg tatttccttt
     4441 gaatcaattt gtaataaatt tattatttct ataaatgtag agatatctat atatctgtga
     4501 cctaaatcaa atatatctga gataaagcta ggtacttttt ctggaactcc tggagttata
     4561 gctattttta tatttttata gttattatca ttcatatatg gaattggtct aatattagtc
     4621 actccaattt tataaaataa acttatagtt tctagagtag tttcatcaga atcatttacg
     4681 attagaacat ttgtaccctg tggtaaagat agtagattat aaattttatc ttccctaaaa
     4741 gttcttctta ccacaatcac ttttttctta tctagtatat tatttataat tttgtctaat
     4801 ctttctactg acattactaa tatcacatca tcatttataa tatcattatc atttatttca
     4861 tttatgaagt atctattaat atttatagaa tctccaaaaa ctaatttcaa attattttct
     4921 aaaaagtcta ttaaatcttt tctactatca tttaccagag ctactgattt tttcatttat
     4981 ttctccttat ataataaata gtttccaaca aaatagttgt ataaagcaat ataattttga
     5041 taatttaaat ttatcaaata aaaaagaaag tgtctataat gactttataa aagttgcatt
     5101 aagacacttt ctttcttata attaaaaatc ttgtcaccaa atccccctaa ttgttgctta
     5161 aaacttaaac tgtatgtata tttttcattt ctattcgatt aaattataat atacacccaa
     5221 aaaaatttat tttaataaat tctattgata atataatttt gtaaatatag aattatatta
     5281 aaataaatta tatatgaaaa tatatatgta taagtttatt ctgcgtgagt agaatgaatt
     5341 tgtcaagttt aatttagttg taattttatc ttttattaaa aataatataa attaaaacat
     5401 ataaataaaa tattttatac aatatattgt aaaggaggta actttgatga aagcagaaaa
     5461 aacaagtata tatccaatta ttaaaaataa tttaaacaaa atatcaattg ttgaatataa
     5521 aaagggtcaa agatttgtag catcagataa agacttacaa gaagtgttct ttataataga
     5581 gggagttgca ttagtggagt gtactactag aggaggaaat aaatttcttg ttgacattgt
     5641 acctgaaaat gaatttatag gcaaaattag ttacatatat gaacataatt tgaaatgtga
     5701 tatatttgca aagactaata taaaattatt tcgatttgaa aaaaatgtat ttgaagagtt
     5761 ttatctcaaa ccagatttta ttgcactatt tcacagaaaa tgtacaagaa gaatatatga
     5821 attatataaa accagaatgg taagagaact attttcttgc acagaagtta ttgcctattg
     5881 tatatcaaat aaacaagaaa ataatgtctg caatataaaa gctattcgta atttcagtga
     5941 aatctattca attagtagaa aaagtagata ccattctctg ctaaagttaa ctgaaaagga
     6001 gatagtaaaa aaaatgggta atacatatga aattttaaac tatgatgaat tacataaatt
     6061 ggcatttgaa gtaaaagaat ttttggaaga tgagtaaaaa ttgttactta atcaaaataa
     6121 agtataataa tttctgtaaa aaatagaaat agtctcactg aaaatcaaaa atactttagt
     6181 tattttcaat gagactgttt agaaatattg tatacttata atgtatagta taattattaa
     6241 tcaatttcct ttactatatt aatagcccat tttttagata atgctctagg aacaccaaat
     6301 ataaacttaa ccacttcttc actataagtg agcaaaacga gtacaaatat aggtgcacct
     6361 ttaaatgcag ctataaaagt tagaggaata gaagctaacc acatacatcc taattctaaa
     6421 aatagagcat atttagtatc tcctccactt cttaaaatac caattataac caatgtatta
     6481 aaagatttaa gagccattag tattcccatt ataaagaata tcttagttat atcagaagct
     6541 aagctatcag aaacactaaa catttttaat aataatggta tatttaaaat taaaataatt
     6601 ccaagtatta aaccagctga aaatacaagt atagaaaact ttttagcata ttcaatggct
     6661 cttttaatat ggtcagctcc aagttcattt ccaagcataa tagatgcacc agatgcaata
     6721 catacagctg tcattatgaa gaaattacct gtactggttg ctatctggct agctgctata
     6781 gcagaagtac cagctcttgc atatgcaaca gaatacagga cagttccaat tgcccataca
     6841 gagtcattta caaatatagg aaagcttttt gatgaaaaag attttataaa aattttatca
     6901 atagctttta gattttttag accaaacttt aatatatagt cttttttata aaggtaaaca
     6961 taagtaatca ttaaaattaa ttcacatatt ctagcaataa cagtagctag agcagcacct
     7021 tttacaccca atgcaggaag accgaagtta ccaaatataa atccataatt taaaataaca
     7081 tttgttacaa gtgcaaatgc actacaaatc atacctaact taggatttct aacacctctt
     7141 gaaccagtac taaatacagt acttacagca attaatggat aactaaatac tgcaatacta
     7201 aagtaatcta tacaaagttt taccacctca gaatcatacg aaaatatgtg tataattggc
     7261 tttggtgtta gtaaagcagg aataacaaat acaaaactta aaacaattgc caatacacaa
     7321 gtaagacctg tgacttttct tatattactc acatccttct ttccataaaa ctgtgaaata
     7381 aaaactccag cacctcctgt gataccagat aaggacatat taaaaagaaa gaaaaattgg
     7441 tttgcaactc caactgaagc tacagatgtt tcccccaaac tacttatcat aattgtatct
     7501 attacattta cagaagtaga aattaagttc tgtatgataa taggtataca taaagataga
     7561 agtattttat aaaatctttt attttcgtct tttaaagaaa atagttcttg caaataatcc
     7621 tctccttttt acataaattc ttcccactca tcatatagag aggcaaggtt attttctaaa
     7681 ttaatttttt cttggctaac ttctttagct ttatctggat tagagtatac ttcttcttga
     7741 cataaaagta tatctaattt ttcaatttca atctctaact tttcaatttc tttttctata
     7801 ttttgtcttt ttattctatt tttcttttct atttctcttt gttctctctc ttttcttttt
     7861 tcatctttta tttgagttct agttttttct tctttttctt ctataatact catctcttgg
     7921 atttgccttt ttttatctat ataataatca taatttccca gatattcagt aattccattt
     7981 tcatctaaaa ctaaaatttt atcaacaaca gtatttagaa agtatctatc atgtgatatt
     8041 gtaaatatag tcccatcata attagttaag gcctcttcaa ggacttcctt agaatcaata
     8101 tctaggtgat tagtaggctc atctaataac aataagtttg catttgatag tataagttta
     8161 agtatggcaa ctctagctct ttctccacca ctcaatgtag aaatcttctt aaaaacttct
     8221 tcatctacaa ataaaaatgc ccctaacata tttctcaaag tagtttgagt aagatgagta
     8281 ttattttccc aaatctcatc aattattgtg ttatctaaat taagagtttt ttgttcctga
     8341 tggaagtagg aaacatgtac attagttcca aatttaatat tgccactaag aggttgtagt
     8401 tcattagtta ttattttaaa taatgtagat ttgccaacac catttgcacc tataagtgca
     8461 actttttccc ctctatatat atctaaatcc aagtctttaa aaagtattct ttctccataa
     8521 cccatagata catctctaat ttgaagaaca tcattaccac tctggacaga aggcgtaaat
     8581 tgtatacgag cttttttcct ataagcttct ggtttatcta aaacatctac tttgtctaat
     8641 gctttttctt tacttcttgc acgctttagg tgtttttctc taccataagc ttttagtctt
     8701 tcaattgatt cttcttgctt ttttatttct ttttgttggt cttcaaattt cttaagctct
     8761 agttcttttt ctattttaga taactcaaca aatttagagt agttaccatt atatacttta
     8821 agctttttat tgtgaacttc aaaaattcta tttacggatt ggtctaagaa atatctatca
     8881 tgagatatta acatgacagt ccctttatat tgttttaaaa atacttctaa ccattctatt
     8941 gcttcagagt ctaagtggtt agtaggttca tcaagtaaca atagagttgg tttcttaaga
     9001 agtaattttc ctaaaagaac tctagttttt tcaccaccag aaagtatgct tattggctta
     9061 tccatatcat tgtcactaaa tcctaatccc tttagaactc ctttggcttc agatttatat
     9121 ccatatccat ttaaatctga aaataattct agtttatgtg aatattcatc catgattttt
     9181 tctataattg gagagtttgt ttttgtactt tcttcagata ttttaacctc caattctctt
     9241 aaataggatt ccatttctat caaattttta aaaacttcta gtacttcttc caaaatagta
     9301 ttatccgaat aaaaattggt attttgttct aaatatccga tttcacaatc tttagaagtg
     9361 taaatatctc cactatcata tccataaatt cctgaaatta ttttaaataa agtagttttt
     9421 ccagtaccat taacaccgat tataccaatt ttatcgcctt catttactgt aaaactaata
     9481 ttttctaata tagaatctat accaaaactt ttatttaagt tattacatga caaaacaatc
     9541 ataaatttct acctccttga actatctaaa attataatgt tttgatataa tataatcaag
     9601 gttatatgta ttagttttga aaaagaaata ttttattgtg aatcaaacaa aaaagaggac
     9661 acttttagga ggtgaagatg ttgggaaata aaaatatatc aatggcagtt ataagaaggc
     9721 tcccaaaata tcatagatat cttggagact tattagatag ggatatacaa agaatatctt
     9781 ctaaagaatt gagtgatata atagggttta ccgcttctca aataagacaa gatttaaaca
     9841 actttggtgg atttggacaa caaggatatg gttataatgt agaagctctt catactgaga
     9901 taggtaaaat tcttgggttg gatcgaccat acaacgcagt tcttgtagga gcaggtaact
     9961 taggacaagc tatagccaat tatgcaggat ttagaaaagc tggattcgag ataaaagctt
    10021 tatttgatgc aaatcctaga atgataggtt taaagataag agagtttgaa gtattagatt
    10081 cagatacttt agaagacttt ataaaaaaca ataatataga tattgctgta ttatgtatac
    10141 ctaaaaatgg agcacaagaa gttattaata gagttgtaaa agctggaatc aaaggtgtat
    10201 ggaattttgc acctttagat ttagaagttc cgaaaggtgt tatagttgaa aatgtaaact
    10261 taacagaaag tttatttacc ttatcgtatt taatgaaaga aggaaagtag acaacttaaa
    10321 catattaata atgagcagtt gtaaagagta ttgagaaagt cagaagattt aatattaaat
    10381 cctctggctt tttgaaatat taggataaaa atttaaataa tgtatggaaa taaacaatat
    10441 taaggagagt ggtttttgtg gaggaattat ggaaaaaggc aattgaattt catggtcatg
    10501 aatgtccagg gttagctata ggatttaaag cagcacttgc agcaaaaaaa tatttaggat
    10561 gtaaacagtc aggagatgag gaaattgtat gtatttctga aaatgatgct tgtggaatag
    10621 atggtataca agtagtgcta agttgcacaa tgggtaaggg aaatttacta cttaggatga
    10681 ctggaaaatc agtatatcat ttctttaata gagaaaatgg aaaatctata aggatatatg
    10741 taaagtcaaa accatttgag atggatagag aaggatacaa aagatattta ttagaaagtg
    10801 attttgaaga aatatttgag attacagata ctaagttaag acttcctgaa aaagcaagaa
    10861 tatttaaatc ccttaaatgt gaagtgtgtg gtgaatttgc atctgagcaa tatataagga
    10921 ttgaagaagg aaagaaagta tgtcttgatt gttttgataa atatgaaaga ttttatgaag
    10981 agtattaaaa ttataaatta gattaaagtt aaaagtatta gatatgtgct aacagtattt
    11041 taagatgaat taggtagcaa cataataaag tatataaata atattaattg aatcatacat
    11101 aattaaaaag aggctgatta gtatttataa aataaaatca gtctctttat gaataacgaa
    11161 ttattattct ggttgaggtg catctacagg acaagctcct gcacaagaac cacagtctat
    11221 acaagaacca gcatctataa catatgcgtc gtctccagct gatatacagc taacagggca
    11281 ttcagcctca caagcaccac agcttataca agcgtcagta attttatatg ccattttgaa
    11341 atcctccctt ttatatgtat tgtaattcaa taacattata acaaatatta ttgaatataa
    11401 caattaaatt aattctacaa caaaaacttt attatgatat tcttgtttta aagttttaca
    11461 ataaagttta atctgcttat atttttattt attttaaaga agtttatttt aaaaataaaa
    11521 ttaaatttaa aaagttatgt attgattata tttaaaaatg ttatactcta aaaaagtata
    11581 ttttatttaa aaatttatta atacaaagga gatttactat gaaaaataaa ttagtttaca
    11641 tatgtattat gagtctattg ctatttgtag taggatgttc taataatcca aataaggaaa
    11701 gtaccattaa aaaagactct aagaataacc aaagccaagt taaagaagaa aaaagagaac
    11761 ctgtgaaaaa agatataact ataagtttcg ctggtgatgt aactatgggg aactataaag
    11821 gttcatcata ttatggcagt tttgaccatg aatttaaaag acaaggagaa gattacaatt
    11881 attttcttaa aaatgtaaaa cctgtatttg aaaaagatga cttaacagtg gttaatttag
    11941 aaggtccact tactacagcg tcaaatgcaa aagaaaaaaa atttgcattt aaaggaaagc
    12001 ctggatatgt aaatatttta aaatctggaa gtgtagaggc agtaagtatt gcaaataatc
    12061 attcagaaga ttattttgaa gaaggaatga aagatacaca gtttatattg gacgagaata
    12121 aaataaatta ttttggatta gggaaagatg ctgttgtaga tgtcaaagga attaaagtgg
    12181 gtctattagg gtataatgga ttatcaactg aatataatga aaaaaattta aaacaaatgg
    12241 aagatgatat taagtcctta aaaaagaaat cggatgttgt tgtagtgtac tttcattggg
    12301 ggatagaatt agattattat ccagataaaa aacaaaaaga ttttgctcat tatgctatag
    12361 acaagggagc agatttagtt atggggtctc acccacatgt gattcaagga atagaaaagt
    12421 ataaaaataa gtatatagct tatagtctag ggaatttttg ctttggagga aataagaatc
    12481 cttctgatac agatagttat atatatcaac aaacctttac tttttcagat aataaattaa
    12541 cttcaattaa agagccaaat ataataccaa cttcaataac atctagtaac tccagaaata
    12601 attatcaacc aaaaatattg gatggaagtg agaaggatag agttttaaat aaacttgaaa
    12661 aaataagcaa agatttaaat aaataaaatg taaaaaaaaa taaatatata tatttatttt
    12721 aataatttat attttgttta gggtatggta aatcgtggta ttaaataagt agattttaat
    12781 agttaaatta tagtcaatta taataaagct atttaatcta cttttttaat ataaaaatat
    12841 aagggggaat ttgtaatggc ttgtaacact tgtaaaatgt gcgaaagtgc agacaagaaa
    12901 ttagaaagtt ttgtagcttc aaaggatgtt gaaacagcat ttcatagaac agaagatcag
    12961 aaagttaagt gtggttttgg acttcagggg gtttgttgta gactctgttc aaatggacca
    13021 tgtagagtaa ctcctaaatc accaagaggt atatgtggag cagatgcaga cactatagta
    13081 gcaagaaact ttttaagagc agtagcatca ggagcagctt gttatttaca tgtggtagaa
    13141 aatacagcta agaatttaaa aaatgttgga ataactaaag gtgttgttaa aggagaaaaa
    13201 acattaaatc aattagcaga gatgtttgga atagaaagtg atgaaaagta tgataaatgt
    13261 ataaaggttg cagacaaagt tatcaacgat ttgtataaat caagagatga taaaatggaa
    13321 ttagtagaaa aaatagctta tgcacctaga gtgaaaaagt ggaaagagtt aggtataatg
    13381 cctggtggtg caaagtcaga agtttttgat gcaatagtaa aatcatcaac aaatttaaat
    13441 agtgacccag tagacatgct agttaattgt ttgaacttag gtatatcaac aggattatat
    13501 ggtcttacac taactaatct tttaaatgat gttatgttgg gagaaccagt tataagaatg
    13561 gctcctgtag gatttaatgt aatagaccca gactacataa atataatgat aacaggccat
    13621 caacattcaa catttgcaaa tttccaagat agattaaaag atgaagatgt tataaaatta
    13681 gctcaatctg ttggtgcaaa aggctttaag ctagtaggat gtacatgtgt tggtcaagat
    13741 ttacaattaa gaggagaaca ttatcaagaa gtatttgctg gacatgcagg taacaatttt
    13801 acaagtgagg ctgttttatc aactggagca atagatatag ttctttcaga atttaactgt
    13861 actataccag ggcttgaacc aatagcagat aaatataaag taaaaatggt ttgtttagat
    13921 gatgttgcta aaaaagcgaa tgcagaatac ataggtttag acagaagtaa attagatgaa
    13981 cttagtaaca ctttaataga aaaagcattg gagtcttata aggagagaag aggaagtatt
    14041 gaaatagata taccaaaaga ccatggattt gaacaatctt taactggtgt aagtgaaaag
    14101 aacctgaaag cgtttttagg agattcatgg aaacctctta ttaatttaat tgcagaagga
    14161 aaaattaaag gtgttgcagc tgttgtagga tgttcaaata tgacagcagg aggacatgat
    14221 gtaaatactg ttgaacttac taaagaattg attaaaaaag atataatagt actatctgca
    14281 ggatgttcta ctggaggatt agaaaatgtt ggtcttatgt cacctggagc agaagaatta
    14341 gcaggagaaa acttaaaaga agtatgtaag actttaggaa taccaccagt attaaacttt
    14401 ggaccatgtc tagctatagg aagactagaa atagtagcaa cagaattagc agctgaactt
    14461 ggaattgacc taccacaatt accattagtg ctttcagctc cacaatggtt ggaagaacaa
    14521 gcattagctg atggagcgtt tggtcttgcc cttggcttac ctcttcattt agctttacct
    14581 ccatttataa caggaggaaa attggtgaca gaagtattaa cagaaaaatt aaaagactta
    14641 acaggaggac atgttatagt gaatccagac cctaaatctt cagctaatca attagaagaa
    14701 ataataatag atagaagaaa aaatttaggg ttaaaggatg tggaatgcaa tgcatagaat
    14761 ttttataaat aaagatttat gtacaggatg taagagctgt gtactagcat gtatgctaaa
    14821 acacaataaa gattatgaca tgtacactct tgatttagaa aatatagata atgatagtag
    14881 agggcatata gaattagata gtagacataa taatccagtt ccaattcttt gtagacactg
    14941 tgatgaacct gaatgtgtat tagcatgtat gagtggtgca atgcataaag atagtgaaag
    15001 tggaatcgtt tcatatgatg aagagaaatg tggttcatgt tatatgtgtg taatgtcttg
    15061 tccatatgga ttattaaagc cagatgatag aagcaagcag aacatactta aatgtgattt
    15121 atgtaaagat gaggagtatc ctagatgtgt agctaattgc ccaagtggag caattgaatt
    15181 acagaaggag gaaaatgatg aattatgtag tgttaggggc tagtgcagct ggtataaatg
    15241 cagtaaaaac attaagagaa ttagataaag attcaaatat agtagtaatt tctaaagatg
    15301 aaaacgtata ttcaagatgt atgttgcatc acgttatatc tgaacacaga actttaaaac
    15361 aaataaattt tgttgatgaa gattttatgg agcaaaataa tgtaaaatgg attgcaggta
    15421 agactgttaa aggtatagac ataaataaaa aggtagttca gacagaagac attactgtaa
    15481 attatgataa acttttaata gctacaggtg catcttctgc tattcctcct ataaaaaatt
    15541 taagagatgg aaattttgta tattctgtga gaaacataga tgatatttat aaaataaaag
    15601 aaaaagcaca aaattctaaa aatgtagtta taataggtgc tggtttagtt ggtatagatg
    15661 cattagtggg attatttaaa tatgagcagt taaatatttc tgtagctttt atggaaaagt
    15721 acattttaga tagacaattg gatgaatata cagcatcagt gtatgaaaat aagttcaaag
    15781 aaaaaggtgt aaaattttac ccaagtgcat caatacaaga aattgtattg gataacagta
    15841 aaaatgttac aggtgttgca ttttcaaatg gtgaagtttt agatgcagac atggtaattg
    15901 tagctacagg ggttaaacca aatgctgact ttttagatgg aacaggaata gaatatgata
    15961 gaggtataat aatagatgat atgtgccaaa caacacaaaa agacatatat gcagcaggag
    16021 atgttgtagg taaaaatgct atttggcctc tggcagtaaa gcaaggaatt gtagctgcct
    16081 ataatatggt tggaaaagat aagaagatag aagatgaatt tgcttttaaa aatagtatga
    16141 atttcatgga tataccaact atttctatag gaatgaatac acctgtagat gatagctata
    16201 aagtattaac cagacatgga attaatgatt ataaaaagtt tgtttttaaa gataatgtaa
    16261 tctatggagc agttatacaa ggagatattt catatgtagg tgtattaact tatttaataa
    16321 aaaataaagt tgaaatatat gatttagaaa ataggatatt tgatatatgt tatgcagact
    16381 tttttaatat aaaagaaaat ggagaatttt gttatagtgt atagtatata cgtggagtaa
    16441 ggtttataaa tacagtaaat caataaatag agttattcta aaataagaaa aatgacaaat
    16501 cttatttaga aatagctcta ttttttgtaa ttaaatttaa attatattca atttgtaaaa
    16561 tattattttg aacaattata catatactct ctagttagag gtaaatcatt ttttatacct
    16621 ttagtgaaaa gtatttgatg tatatctata ataccagtgt tgaaagcagc agcacaagaa
    16681 caaaggtata aatcccacat tcttataaac ttatcatcaa acatttcttt aatctttgga
    16741 agattctcag agaaattttt ttgccagtat agtaaggttt ttacataatg taatcttaaa
    16801 ctttctacgt ctatagtatg atatttgtaa tcactacaaa tatgtatcat ttctcttagg
    16861 ctaggaataa ctcctcctgg gaaaatatat ttttttatcc aagggtctcc ataagattcc
    16921 tgcaatccac ttatatagtg aagtaaaaac aatccaccat cttttaaaac agcatttacg
    16981 tttttaatga aaagttcata gttttctcta ccaacatgtt caagcatccc aacgcttaca
    17041 actctatcaa acataaggtc tgattttttt aattctctat agtccataag tcttacctca
    17101 agatagtctt gaaggttctc ttctttaatt cttgcgttaa attccttaaa ttgctcttcg
    17161 ctaagtgtaa ttccaacgcc gtgaatttta tatttttttg cagcttctat tagtaaaaat
    17221 ccccaaccac aaccaatatc gagaaggctc ataccttcct ctaaatttag tttagttaat
    17281 atgtggtgaa ctttattgac ttgagcatca taaagagtat cattatcatt tctaaagtat
    17341 gcacaagaat aactaagtgt cttatctaac cataagctat aaaaatcgtt gccaatgtca
    17401 tagtgggcat gtatttcttt cttttgatgt ctcattgaat ttgatgagtg caatagatgt
    17461 tttacagctg aattatcaat attgaatttg tctatttgtt ttaaaagtag atttaaagtt
    17521 aagaataaat ctccctcaac ttcaatgtat ctatttatgt aggcttctcc aagtgctaaa
    17581 gatgtacttt ttaatagttc agacttgcta atatcattat ttactataac tttgaactga
    17641 gggaagccct ctccaattag aaattcttcc tcatctttaa gcctaactag aaatggtgtt
    17701 tcaccaaatt tctctaaata gtcttttaaa aaattttttg taatattcat attaccagct
    17761 ccttctaatg taaatccagt aagtttccct gcttaaaata tattatagtc ctttttttaa
    17821 aaaagtaaaa ttttttcaat aaatttttta tttaaaggaa atttattttt tgtaaagaaa
    17881 tttcatgttg tagtatattt tttgcttata aattttggta ctggatataa tattaaactg
    17941 ttaatgatat aataaattta atctataaag taaataaaat tttgtaataa atagttaaag
    18001 ctattttata aggagatata tgatggaagt atatgctttg gttgggtcaa gtggcacagg
    18061 gaagagcttt aaggctttag agttcgcata tgaaaatgat atagaatata taattgatga
    18121 tgggatttta atatacaaaa acaaggtttt agctgggata tcggcaaaac aagcaaatac
    18181 tactatagaa gcagttaaaa gggctatttt ttataatctg gagcatagac aagaagttag
    18241 agaagcaatt aaaaaagaaa atataagaaa aattcttatt attgggacat caaaaaaaat
    18301 ggttaatcaa attgtagaga gattgtcaat tggaagggtt tgcaagttta taaatataca
    18361 agatataagt actaaatatg aaatagaaat agcaaaacaa gctagaaaag aaggaaatca
    18421 tgtaattcct gttccagcag ttgagataaa aagtatggca agtggtctta gtataaattc
    18481 tttgaagaga ctttttagaa aaggaaataa taggaatatg gtagttttag aaaaaacaat
    18541 tataagacca acttttagtt atataggaaa gttttatatt agtgctgatg taattaaaca
    18601 gattattgaa tatgaagtat acaactttgg aagcattgat agaataaata aaatcaatat
    18661 agagaattgc aataatttta tgaatatttt tgtaagtata aatatcaatg atttagaaac
    18721 tataaaaaaa attgaagaca tacaacagac aataaaaaag tctattgaga aaatgacttt
    18781 agttaatgta caaaaaatag atattaacat tcataaaatt aaaaaaataa acttcctcca
    18841 atgttaaaaa ataaactgag aaaatgatat actaatttgc aataaagtgt gctataatta
    18901 aactgtaaat ttaggaaatg gttttttcta aatggagtat acataattat aacgtattta
    18961 taagggggct ttcaaatgtc aggaaaagat gtaaatgtct tcgagatggc gcaatctcaa
    19021 gtaaaaaatg catgtgataa attaggtatg gaaccagcag tttatgaatt attaaaagaa
    19081 cctatgagag ttatagaggt ttcaattcca gtaaaaatgg atgatggttc tataaaaact
    19141 tttaaaggat ttagatcaca acataatgat gcagtagggc caacaaaagg tggaataaga
    19201 ttccatcaaa atgtttcaag agacgaagta aaagctttat ctatatggat gactttcaag
    19261 tgttctgtaa caggtatacc atatggtgga ggtaaaggtg gaataatagt agatccatca
    19321 actttatctc aaggtgaatt agaaagatta agtagaggat acatagatgg aatatataaa
    19381 ttaataggtg agaaagttga cgttcctgca ccagacgtaa atactaatgg tcaaataatg
    19441 tcttggatgg ttgatgagta caataaatta actggacaaa gttctatagg tgttataact
    19501 ggtaagccag ttgaatttgg tggttcttta ggaagaacag ctgctactgg atttggtgtt
    19561 gctgttactg caagagaagc tgctgctaaa ttaggaatag atatgaaaaa agctaaaata
    19621 gctgttcaag gtataggaaa cgttggttct tatacagttc ttaactgtga aaaacttggt
    19681 ggtactgttg tagctatggc tgaatggtgt aaatcagaag gttcttatgc tatatacaat
    19741 gaaaatggtt tagatggtca agctatgtta gattatatga aagaacatgg taacttatta
    19801 aacttcccag gagctaagag aatatcttta gaagagttct gggcttcaga tgttgatata
    19861 gtaataccag ctgcattaga aaactctata actaaagaag ttgctgaatc tataaaagct
    19921 aaattagttt gtgaggctgc taatggacca actactccag aggctgatga agtatttgct
    19981 gaaagaggaa tagttcttac tccagatata ttaactaatg ctggtggagt tacagtttct
    20041 tacttcgagt gggtacaaaa cttatatgga tactactggt cagaagaaga agtagagcaa
    20101 aaagaagaaa tagctatggt taaagcattt gaatctattt ggaaaatcaa agaagagtac
    20161 aatgttacaa tgagagaagc tgcttacatg cattcaatta agaaagttgc tgaagctatg
    20221 aaattaagag gatggtacta agatttactc ttaataaata ataggatata attatttaag
    20281 atataaaggg gctgtacata gtacagccct taaattatgt ggcttacaat gaagtaagct
    20341 atttttgttt taacttataa ataacttgat aaattatgta atttagaatg tattttttac
    20401 caccacatga ttacaaatta gaggagttag attgaatttt tagacatgaa cttatgcatt
    20461 ttaaaagcaa agaccttaat ttaaagtatc tagtattatt tttaaaaata gtatattggt
    20521 ttaatccatt tatatgtata atggaaaaat atatggattt ggattgtgaa ctttattgtg
    20581 atgagagagt tttaaaaaat tgtactgtac aacaaaagca agaatatgct ctaactattg
    20641 taaattcaat gaaaagaagt acaaattttt caaataaatt tgtagctgga ttaaataaac
    20701 aatctgatat tagaaaaaga gtaataaaca tgttcagtga aaaacataaa aatggtatct
    20761 taagggcttt gattataagc ttgttttctt ctattacatt ttttaaattc gattcaacta
    20821 gtattattga agagaatgat ttcttagaga ggtcaagata tacgtttgaa ggaaggcaag
    20881 aagacatgtt tgtaacaata gattttatat atgcagatgt acctataaaa cttagaaaag
    20941 agcatgaaga aaactgtaga gtcaatggaa tagtaccaaa agattcagat tcaatagaaa
    21001 ttattcctag attttataaa gattgtatgg atttaaaaaa ataagttaga cttaatattg
    21061 atgttgatat tatgaatttt ttatatttaa gcaattgaaa attttattat agattcaatt
    21121 ttatatgtgt ttaagtatga aatttataga tagttgaaat aattaaatat gattgatgca
    21181 taggaatcat tactaagcta taattaatta taggggagtt taaataaatt atgagttaca
    21241 caattataaa atgataaaaa tagttatctg ttacaaaaaa tgtatctttt taattaataa
    21301 agataatatg aaaacttaaa aggcaaataa acttaaatat aaaatactct taaataagat
    21361 aggagaaaaa tgtatgtcta actttattct aaatgggatt tttagaacag ttatagtaag
    21421 tagtctaagc attcttctga tattgatttt caagaaaaat gtatttaaaa gatttagcaa
    21481 aaaatttaat tattatatat ggatgatagt tgttattaaa ttatttttac catttacata
    21541 ttatactttt acaataaata tcttgagaag taaaaaacac attaatatta ataacataaa
    21601 tttagaggga tttaataatg tttcaactat aagtaataat attatattgt acacttggat
    21661 tataactgta gtagtgtatt taatttacac tatatttaaa tatataaaac ttaaaaattt
    21721 aataaatgac ttatcttatg atgttgatga tagagagatt gtaaatcttt ataaaaatat
    21781 attagaagaa tttaatataa caaaagatat aaaactcaaa tactcttatg aagttgaaac
    21841 accagcattt tttaattcat gtgtactttt gccaccacat gagtataaat tgaaagagtt
    21901 agactggatt tttagacatg agcttatgca ttttaagagt agggaccttt atttaaaata
    21961 tgtaatccta tttttaaaaa cagtatattg gtttaatcca tttatatata taatggataa
    22021 acatatagat ttagattgtg aactttattg tgatgaaaga gttttaaaaa atcgaaattc
    22081 agaggaaaaa aaggattatg ctttaacaat tctaaatgca atgagaaaag gttcaaatac
    22141 ttcaaataaa tttatagctg gacttcataa gcaatctgat attaaaaaga gagttatgaa
    22201 tatgtttaat gaaaagtaca agaatggtat attaatggca ttaactttat gtttattatc
    22261 ttctattact tttttgaagg ttaattcaac tagtattgtt aatcttaatg tattacctaa
    22321 aggatttgaa gacttacgaa ctgttagcag tatacgtttt tcagttatag gatttacata
    22381 tgcagatgct cctaaaaata tgagggaaga atataaaata aattgtgaat cattaggttt
    22441 gatacctaaa gactctgata aaatagaagt tggtatagaa tattatgaca gtctattgaa
    22501 tgattaaata aatattattt taacagatat taattataaa gttttgaata gtaatttgtt
    22561 gagaaataaa tatatcctta taaaaataga tacttatata ccaagaataa aaaatagtca
    22621 agatagtaca caaggatata cttataaaga acttaaataa gtgaaataaa ttattaatta
    22681 taaggtttat aattttaaaa aattagataa attcaggtgt aaaatactat aaaacacaat
    22741 tcggaaaagt gtatgttgaa cttcatttaa aatggaattt caaagttgct atagtaagta
    22801 gtttaagcat tcttcttata ttaatctttg agaataatgt atttaaaaaa gttagtaaaa
    22861 aatttaacta ttgtatatta atgatagttg ctattgatat attatctaaa aaaattaagt
    22921 atgaaaagat tactgctagg acaatctttt catacttaat ttttaatatt tatattatcc
    22981 aaaattttca tctttttaac ttagaatttg agaatcacta ttaggaaata ggctttgatt
    23041 tatgtagggt taaaaatttc acagaatatc taactttata ctaaatccat caaaaaatat
    23101 acgccataca aaagcgcata aaaaaacaca taaaacaata tttatgcgca ctttaacctc
    23161 gaatggtaca aaattcttta cttttacaga cccatattat ataattaagt aaataatgta
    23221 tatttaggtg acatattgga taagctaatt tatttatttt attaatataa taatactata
    23281 aaagttatat aaagtggttt acaaaaaagt tacagataga gcttttatta atgtattgtg
    23341 tattagtatt ttatccttag gtacacttca cctacaaaat agaatcataa gataattatg
    23401 ggaaaatttt aggaggttta tcgtgaagaa aaagatatta ataccagtat ttgcatcagt
    23461 tatggcatta tcagtaagtt caattgtaaa tgcagatgaa gtaaatgatt catctcaaaa
    23521 taaggatgat aaaacaaata ctgaattaaa tttaggtgaa tataaagaag taaaatataa
    23581 agttgcaaaa ataaaagatg gagtagctat aaagataaga gaagaaggtc aagttcaaaa
    23641 tatagcttat tctggagatg aatttactgt tttaggaact caaggagaat gggttaaggt
    23701 taaagttgaa gatggtgaag gttggttagc tactagatat gtagatatat ctgaaggtgt
    23761 aggatatact aacgctgata aagtaaactt aagaaaagat aagagtgaaa gttcagaagt
    23821 aattgaagag ttggaaaaag gaagttcttt attggttttg gaagataatg gagattggtt
    23881 aaaagttaaa gatggggaaa cagaaggata tgtaaaatca tcttatatat ctgataaagc
    23941 tcctgtaata gaagagccac aagtgaaccc aagtattaac caaaatgtag attctacaaa
    24001 tactaatcaa caaagcaatg caaatactaa tcaacaaaat aataattcaa atgtgccaac
    24061 agctaactct aatgcagttc aagctgtact taacttggca tattctaagc aaggttgccc
    24121 atatgtttgg ggagcagaag gaccaaacac ttttgactgt tctggattta cacaatatgt
    24181 gtatagaaat gcagtaggaa agaatatacc aagaacatct aaagcacaat ctaaatatgg
    24241 acaaactgta agtaaagcaa atttacaacc aggagactta gtattcttca caactaatgg
    24301 ttctggttct gttagtcatg ttggaatata tgtaggtggg ggtaatatga tacactcacc
    24361 atcaacagga aaaacagtta gtgtaactag tataaattca tcatactata cagcaagatt
    24421 tgttacagca aaaagaatat tataatcggt ttgtaatatg acaaattgat aaatacataa
    24481 atatatataa acgctctata ttatatttaa tttaatcatg gaaacaaaat aatgattata
    24541 ttttaaatgt actatagagc gtttttgtat cactttaaat ctaatcaaga ttcaagttaa
    24601 tatgactgtt aaataggagc cttttcatat gatgattatt tgtgcaatcc cttgacagga
    24661 tagggtaaag gtggtaagct actaacagta caaaaatact tttaatttta gttcagagag
    24721 gctaaaaaag gaggacgaaa gatgttaaaa tcaagaaatt taatccaacc agaagacttt
    24781 tcaatagaag aaatagatga aatattagaa ttagctcaaa agataattga caatccgtca
    24841 aaatattctc gtatatgtga aggaaaacta ttagctacat tattttatga accatcaaca
    24901 agaacaagat taagctttga atctgctatg aacagattag gaggacgtgt agtaggcttt
    24961 tcagagccaa attcatcctc tgcatcaaaa ggtgaaactc taggagatac aatgaggata
    25021 gtatctggtt atgttgatat aatagctatg agacatcctc aatcaggtgc agcttcagaa
    25081 gcagctagat atacggaagt gccatttata aatgcaggag atggcaaaaa ccagcatcca
    25141 actcaaacac ttacagactt acttactata aaatcattaa aaggaacttt agaaagccat
    25201 acaataggat tatgtggtga tttaaaatat ggaagaactg tgcattcttt ggttaaggct
    25261 atggcaagat ataaaaatac aaagtttgta tttatagcgc ctgaagagtt aaaaatgcca
    25321 gattatataa aagacgctat aaagggacat gcatattatg aaactaataa tttggatgat
    25381 gttataggaa gcttggatgt tttatatatg actagagttc aacaagaaag atttgaagat
    25441 aaatcagagt atgaaagact taaaaactat tatattttaa ataaagttaa attagaaaag
    25501 gcgtctaaag atatgttagt aatgcatcca ttaccaagag taaatgaaat agacatagat
    25561 gtagatagtg atgatagagc tgtatatttt aaacaagcta aatatggaat gtatgttaga
    25621 atggctttaa taataaaact tttaggtata agtgaagact aatagttatc atataatagt
    25681 aaaacaaagg agattttggg atgtataaaa ttctggaaaa tatatatata ggcgaagata
    25741 tgtatagaat gaaagtaaaa ggtaattttg aaggtaaaat gggacaattc tatatgctaa
    25801 gagcatggga tacttatcca gtactttcaa gacctataag tatacatgat atagatgaag
    25861 aagggataac ttttctttat aaagtagtag gagaaggaac tcaaattttg agtaacctaa
    25921 aggtaaatga tactataaaa ttagaaggcc cttatggtaa tggatatgct aaggttgatg
    25981 gaaaggtagc tctagtagga ggaggaatcg gtgtagctcc actatatttg gttgctaaaa
    26041 atataaaaaa ttgtgacgca tatcttgggt ttagagaaga tgttatatta gaaaatgaat
    26101 ataagcaagt atgtaataaa gtctatacaa cagtaggaaa tacttttgta acagatataa
    26161 ttgatgttga gaaatatgat tatatattaa catgtggtcc aactccaatg atggaaaaat
    26221 tagttaagat ggtagaaggt actaaaacga gaattatggt atctcttgaa aaccatatgg
    26281 cttgtggagt aggtgcctgt ttagtgtgta cgtgtaaaac taatgaagga aataaaaaga
    26341 cttgtaaaga tggtccagtg ttctggggag aggacgtgat ttttaatggg taatttaagt
    26401 gtgaaatttg gaagtgtaga atttaaaaat ccagtaataa tggcgtctgg aacttttgga
    26461 tttggaaaag aatataatga gatttatgat atacaaaaac ttggtggtat aagtagcaaa
    26521 ggcattactt taaataaaaa acctggtaac aatggtatga gagttcatga gacaccatct
    26581 ggaatgatga atagtgttgg gcttgaaaac cctggagttc aaggatttat agataatgaa
    26641 ctaccttttt ttagtaaact agacttagtc agaatagcta atgttggagg tgggacatta
    26701 gaagactact tattaggggt tcaaatgtta aatgataaac ctatagacat tattgaatta
    26761 aatatatctt gcccaaatgt caaagctggt ggaatggctt ttggtataaa aaataaagta
    26821 gcaagagaag ttgttcgtga ggtaagaaac ataacgaaac tgcctttagt aataaaatta
    26881 tcacctaatg cagaagacat tgtaggaatg gcaaaagttt gtgaagaaga aggcgctgat
    26941 ggagtttcat tagttaatac ttttaaagca atggcaattg atataaaaaa tagaagacca
    27001 gtatttgaaa atgtatatgc tgggttatct ggaccagcta taaaacctat agctttaaga
    27061 atggtacatg aagtgtgtaa aaatgtaaat atacctgtaa tgggaatggg cggaataact
    27121 aaagctacag atgctataga atttataatg gcaggagcta cttgtatcca agttggaact
    27181 gcaaacttta tgaatcctag aataggtatt gaaataatag atggaattaa tgaatttatg
    27241 gatagagaag gaataaaaag tttagacgaa ataagaggaa ttatataagg cagaataaat
    27301 agtattttaa agataaaaaa tatagattaa ataaacaata attttataac attaacatgg
    27361 aggaaaaata atatgagtaa agtagatgta gtagatatat taaagaaaag tgatgcattg
    27421 ttggagggac attttttatt atcttcagga aaacacagta atagatacgt acaatgtgca
    27481 aaagtattaa gattcccaga atatgctgct gaggtattaa gtacagttgt tgaacaaata
    27541 aaagacttag atatagactt agtagtagga ccagctatgg gcggagtaat agtttcttat
    27601 gagttaggaa gacaattagg aaaagaagct gtatttactg agagaaaaga caatacaatg
    27661 gagttaagaa gaggatttga agttaaaaaa ggagcaaaga taataattgc tgaagacgtt
    27721 gtaactactg gtaaatcaac tatggagaca aaaagagtat tagaagcctt aggtggagaa
    27781 gttgtaggtg ttgcatgtat agcagataga actaatcatg atataggtat gcctatatat
    27841 agtgctataa aacttgatat tcaagtttat gaatctgatg agtgtccttt atgtaaggaa
    27901 ggaaaattac cagttgttaa gcctggaagt agagagttca aggaattagg aatgtaataa
    27961 aaacttaatt attcatagtg ttacttaaaa aaggtaaatt ttttagagta aaaacttgta
    28021 atataacata taacttaaaa ataaaaagca gttagcctta taaatgaagg ttagctgttt
    28081 tttttgttaa aaatatattt acaaaataag agatagtagc agtacaatat aagtataaga
    28141 taaaaagtag tatatattag ggaggggtaa gtatgaaaaa tcaattaaat gaaaatcata
    28201 aaaaggttgt attttttgga gtaggcgctg taggagctac ttttgcagaa caatttttta
    28261 actctaaata tgattttaaa attctttgtg ataatgaaag aaagaaaaga tatttagaag
    28321 aaggatttat aataaatgga aaaaggtatg attttgatta tgtaactaaa gatgagtata
    28381 agcaagaggc tgattttata attataggtt taaaatataa taatttaaaa gaaaatataa
    28441 aagaattaga tggattagtt ggaaaaaata cagttataat gtctctgcta aatggagttg
    28501 atagcgaaga gataatagga gaaagatttg gaattgaaaa aatggtatat tcatatgtta
    28561 ccaatataga tgcaaagaaa atcaataata atattataca cactactaat ggggtaattg
    28621 tatttggtaa caaagataat agtgaagata gaaaaactaa tataataacc gaagtctttg
    28681 atgatgtaaa tatagaatat attttatcaa aagatattca acgagatatg tggtggaagt
    28741 acatggttaa tattggtgta aatcaaactt cagctatact tggtgcacct tatggagttt
    28801 ttcagagttc tgagcattta agagaattag caaaatctgc aatgagggaa gttgttgcta
    28861 tagcacaagc aaaagacata tctcttacag aagatgatgt ggaaaattca ttacatagaa
    28921 tactagaaca ttcaaaagaa ggaagaacat caatgcttca agatgtggaa gctcatagac
    28981 ttacagaagt agatatgttt tctaagaata tctgcaaact tggaaaaaaa tataacatac
    29041 ctactcctat aaatcagact ttcttttata tgataaaagt aattgaaagt agattttaaa
    29101 gggtctttgt aagaaatatt gataattatt gattttaaat tttattaatg ttatatacta
    29161 tttaagttac taaatatttt taactcttca atgaaaatag ttgctgcttt tgataaagtt
    29221 atatcttttg gaaatatata tgagaaatgt ctgttgtagc tgcttccaag ctctatgaca
    29281 gacagctcat tgtttagaac aggtaagctt gtcactagat tagagactat tgtaattcct
    29341 aaattatttc taacagattc tttgacagca taattacttc caaaaaccat catacttttt
    29401 ggaataattt ctttaacact taaaaacata tctaaataat ctctggttcc agaaccatcc
    29461 tccctgacta tccatttttg gttttgaagt ttatcaaaac taaaatcttt taataaatga
    29521 ctcttgtatg gaagtgctaa aaccatcttg tcttcaaaaa aatattcttg aataaatgaa
    29581 ggggaagagc aagtgccttc aataagacca atatctaaaa tataatcctt aacatgagaa
    29641 gatacgattg atgtattttt tataaaaact tctacatcta tgtcaggata ctttttagaa
    29701 aataatgcta gaaaatttgg tagaatatac tctccaatag ttaaacttgc accaatcttt
    29761 aagtgaccag ttatagcatc tgaaacttgg cttacatcat gataagtaat atttaaaaga
    29821 tttaatattt ctttagctct tttgtacaat atttgtccac tttctgttat aaaaatggtt
    29881 ttttgtttaa tagacctgtt tataagcaca actttaaaat aactttctag attttttatg
    29941 tgtttactta cacttggttg tgatagattt aagtattccc cagctttagt aaagtttttg
    30001 tattcaacaa cagctacaaa tgtttttaat tcttcaaaca attttttgtc tcctttacct
    30061 aatgaaattt tacatttttt atatactttc tacagataat tatactatat ataataacca
    30121 ttaaaaacat taatcattac gatattatat atttatttta cttatggtta ttacaattat
    30181 atactattgc tatgaagaaa actatattaa ttataaggta ggtatattat gttaaagaaa
    30241 aaaagcagtt tgacattaaa aactataagg gaaatattac caggactgtt tgtatcagta
    30301 ttagtaggtt atattagtat atttatttct attttaattc ctaaggtagg ggcagcatca
    30361 atttctatat ttttaggtat gtttgtagga aacctatttt taaatcaaaa ggtatttcaa
    30421 aaaggctata aattttcaga gacagatttg ttatcatatt caatagtact attaggagca
    30481 acactcagtg tatctacctt aatagactta aaagtttcag gaattttatt tataatattg
    30541 caaatgacaa taacaataat agctgcactt tatataggta aaaaactagg atttgaagaa
    30601 aactttagat ttctaatggc aagtggaaat gcagtatgtg gttcttctgc tataggagca
    30661 acagttccag tagttaatgc gacagataaa gaaaagggaa ttgcagtaac tattgtaaat
    30721 gttactggta tattccttat gtttttactt ccaataatat ctcaattttt atatagccat
    30781 gaacttgtaa aaacgtcagc aatgataggt ggaactttgc aatcaattgg tcaggttgta
    30841 gcaagtggag caattgttgg agaaaatgta aaagatttgg ctactatttt taagatagta
    30901 agagtaatat ttctagttgt tgttgtatta gtatttggac atatgaaaaa tcagtctaat
    30961 agggaaatcc ttgaagaaga aaaggatgag ataaaaaaga aaaaagtaac tataccatgg
    31021 tatgtaattg gattctttat aacttgtgca cttttttcaa tgaatattat accaaaagaa
    31081 gtatctgtgt tgtgcaaaga aataagtaac aaactagaaa taatagcttt agcagcaatt
    31141 ggattaaagg ttaatgtaaa agatttagtg aaacaaggga aagaagtttc tttatatggt
    31201 ttatttgtag gtacagtaca agttgtatca gctgtagttt taataaaaat atttatatag
    31261 cataaaaata gtatttgtaa tattaatttg attaattaaa ataatatgat actaataatt
    31321 tataatataa ataaaaaatg aatttcgata agtttaagtg ggtataaact tatagaaatt
    31381 cattttttat ttaaggtaaa aatttaaagt aaaatattat tttacataat aataacctta
    31441 taaattttga aattacttat ctttgtgtta aaataagtaa aatgcacata ttagagctaa
    31501 gtttttaagc aaataaacaa aatgaaaata taaatattaa ggagatttgg tatatgaatg
    31561 tttatgaaaa aggtaatgga gttatactag aaggagtaac agattttgac ccagtacata
    31621 tatttgagtg tggtcaatgt tttagatggc acaaacaaaa agatggctca tacactggag
    31681 ttgcaaaggg gagaatatta aatgtcaaaa aagagaatga taaaatatat ttaaataata
    31741 ctaatttaaa ggagtttaac agcatttggt ataattactt tgatttagga actgattata
    31801 cagaaataaa gaataagcta aaaaatatgg atgaatattt aaataaggct actgaatttg
    31861 gatggggaat aagaatatta agacaagatg gatgggaaat gcttatatca tttataattt
    31921 cctctaataa taggattcct atgatacaaa gagctataga aaatttatca agaaaatttg
    31981 gtaagtatat aggtgaatat gaaggtaatg aatactatgc attcccaaca ccagaagaac
    32041 taaataaggc ctcacaagag gaaataagag catgtcaaac aggttttaga gataagtaca
    32101 taaaaagtac tacacaggaa gttatagaga ataatgataa ggtatctgaa tatacaaatt
    32161 taagtacaga ggattgtaga aaagatcttt taaaatttaa tggagtagga ccaaaagtat
    32221 gtgattgtat tgcattattt ggtatgcaaa aatatgattc ttttccagtt gatgtttggg
    32281 ttaagagagt tatgcaagaa ttttatatag atgaagacat gagtttacct aaaatgagaa
    32341 cttatggaat cgataaattt aaagaaatgt ctggatttgc tcaacagtac ttattttact
    32401 atgcaagaga actcggtata ggaaaataaa ttaaggggga gaaattatgg gagtaatagg
    32461 aacaatattt ctgttctatt taattatatc atatctagct ggagccataa tttctgtgat
    32521 tatattactt gaaaatagag accctgcaaa aacaatgtct tggttattga tgtttattat
    32581 atttccaggt gtaggtttaa tgatatatgc tatttcaggt agaaatatta gaaaaagaaa
    32641 attatttaag actcaaaaac tagctaataa tataaaagaa aagaaactat ttgatacatt
    32701 agaaaagata acagaaatag ttgaattaga aaaagaatct ataaaacaaa ataagctttt
    32761 aagagatgaa gaggatggta gttatagaaa aagggtaata aatatgttat taaagactgg
    32821 tatgtttccc tttactaaaa ataataaagt ggatgtgttt gtagatggaa atgaaaagtt
    32881 taaaagactt atagaagata taagagaagc taaagaccat attcatttag agtactttat
    32941 catcaaagat agtgaaatag gtaggttgtt aaaagaagaa cttataaaaa aggctaaaga
    33001 aggcattaag ataagaattt tatatgatga tgtaggatgc tggagatttt ggtttaatag
    33061 aaaatttttc cgagaaatga gggaagtagg tatagaaatt gcagcatttt tacctacaaa
    33121 atttcctata ataggaggga aattaaacta tagaaatcac agaaaaattg ttgttattga
    33181 tggaataatt ggatatacag gtggaataaa tataggagat gaatatctag gcaaaaatga
    33241 taagtttgga tattggagag atactcatat taggattaag ggtatttctg tatacatgct
    33301 tcaaatgaca tttctgattg attggtatta tacaacaaaa gaagtattag taactaaaaa
    33361 ttattttcca agtgtaggga atattggtga aagtatgata caagttgttg cgagtggtcc
    33421 agacagtgat tgggaagata ttcattatgc ttatttttct gcaatatgtc aggctagaaa
    33481 aaatgtttat atagaaactc catattttat tccagatgaa agtctactaa aagctataaa
    33541 gagtgctgct cttagtggag ttgatgtgag aataatattt cctaaaattg cagaccacaa
    33601 gatagtaaat atagcatctt attcatattt tgaagaaata ttgagagcag gtggtaaagt
    33661 ttatttatat aataaaggat ttatacattc taaagtcgta ataatagatg ataaaattgc
    33721 ttctgcaggt acagctaata tggatttaag aagttttatg cttaactttg aagtaaatgc
    33781 atttatctat gatgaagaag ttattagagt aatgacagat gatttctttg aggatttaag
    33841 tcactgtgaa gaacttaatt tggaggtatt taagaataga aatatcatac aaaaaatcaa
    33901 agaatctgta gcaagattat tttcaccaat attataaaaa attattaaaa aaggtattga
    33961 aaattatgtg tgattggtat attataataa ttgttagcac tctattaggt agagtgataa
    34021 taaataaaaa gtatatatat aaatctacat atataagtag tattaattta aaaataacta
    34081 aaaacatagg cgaactagtc tatacttaat attttaggag gcgtgaataa tgaaaataag
    34141 accattagct gacagagtag taattaaaaa agtagaagca gaagaaaaaa ctgcaagtgg
    34201 aatagtttta ccaggagcag ctaaagagca acctcaaata gctgaagttg tagaagttgg
    34261 accaggtgga atagttgaag ggaaagaaat aaaaatggaa ttgacaatag gagataaggt
    34321 tatattccaa aaatattctg gaacagaagt taagatagaa ggacaagaat acacaatact
    34381 aagacagagt gatgtattag ctgttattga ataaatatag aataaattta ttaggagggg
    34441 tttaaaatgg ctaaagaaat taaattttca gaggaaacaa gaagagcttt agaagctggt
    34501 gtaaataaat tagcagatac agtaaaagta acattaggac ctaaaggaag aaatgttata
    34561 ttagataaaa aatttggttc tccacttata actaatgatg gagtaactat agcaaaagag
    34621 atagagttag aagatagatt tgaaaatatg ggtgctcaat tagttaaaga agtagcaact
    34681 aaaactaatg atgtagctgg agatggtact acaactgcta cagttttagc acaagctata
    34741 ataagagaag gtttaaagaa tgtaacagca ggggctaacc caatactttt aagaaaagga
    34801 atacaaaaag cagtaacagt agcagtagaa gaattaaaaa atcaatcaag aatagtagaa
    34861 acacaagagg ctatatctca agttgcttct atatctgctg gtgatgaaga agttggaaaa
    34921 ttaatagcag aagctatgga aatcgtaggt aaagatggag ttataactgt tgaagagtct
    34981 caaactatga atactgaatt agatgctgtt gaaggtatgc agtttgatag aggatttgtt
    35041 tctgcatata tggttacaga tgtagataaa atggaagcag ttttaaatga cccatatata
    35101 ttaattactg ataaaaaaat atctaacata caagaattat taccagttct tgaacaaata
    35161 gttcaacaag gtaaaaagtt attgataata gctgaagatg tagaaggtga agcattatct
    35221 acattagtag taaataaatt aagaggaaca tttgatgtag tagcagttaa agctccaggc
    35281 tttggagata gaagaaaaga aatgcttcaa gatatagcaa tacttacagg tgctcaagta
    35341 atatcagaag aattaggtta tgatttaaaa gaagctgatt tatctatgtt aggtagagct
    35401 tcatctgtta aggtaactaa agaaaatact acaatagtag atggttctgg agataaaaaa
    35461 tctatagaag atagagtaac tcaaattaaa catcaagtag agcaaactac ttcagatttt
    35521 gatagagaaa aattaatgga aagattagct aaacttgctg gaggagtagc tgttgtaaaa
    35581 gttggagctg ctacagaagt agagttaaaa gaaagaaagt taagaataga agatgccctt
    35641 aatgcaacta gagcagctgt agaagaagga atagtagctg gtggaggaac tgcttttgtt
    35701 agtgttatac cagcaatagg aacactaatt gagagtttag aaggcgaagt taaactaggt
    35761 gctcaaatag ttaaaaaagc gttagaagag ccattaagac aaatagctat aaatgctggt
    35821 cttgaaggtg ctgtaattgt gcaaaatgtt gttaattctg aagcagaaac tggatttgac
    35881 gcattgaatg aaaaatatgt aaatatgata gaagctggta tagttgaccc aactaaagtt
    35941 agtagaagtg ctttacaaaa tgctgcatca atagcaagta ctttcttaac tactgaagct
    36001 gctgttgctg accttcctga aaaagaagat gcaggaatgc caggaatggg tggcggaatg
    36061 ccaggtatga tgtaatttaa aaaatttaaa atataaattt taatataaag aactatttcg
    36121 acttagattt taaactctaa gttgaaatag ttcttttgta tatatctaat ttttgtatat
    36181 ttataaattc aatacacata tttagataat cagtatctag tttgtaataa tattttagag
    36241 tagtattgtt attttttagg ttatgatttt agatatatat taattaaggt ataattgttg
    36301 atagagaata aatcaaattt aaaaggatgc gataatatgg acttaagaaa attgcttgaa
    36361 gaagttaaaa atgaaaatat tgatatagat tttgcgttag aaaagttaaa agatttacct
    36421 tatgaagaca ttggatatgc aaatatagac catcatagag aattaagaaa tgggtatccg
    36481 gaagttattt attgtgaagg taagagtgat gagcatattt tagggattat tgataaaatg
    36541 aatcaaaaaa gttctaacat attggggaca aggtgtagaa aagaaacttt tctgaaaatt
    36601 aaagaaatct ataatcatgc tgagtacgaa gagttgtcta aaattcttaa gattcaaaat
    36661 catgatatag agaatatagg aaaaggtaaa attgtgattg ctacaggggg aacttctgat
    36721 ataccagtag cagacgaagc ctaccataca gctaagtttt taggcaatga tgttgataga
    36781 atatatgatg ttggagtggc aggtatacat aggcttttaa ataaaagaca taaaatagat
    36841 ggtgctagag taatagtagc tgttgcaggt atggaaggtg ctcttgcaag tgtaattggt
    36901 ggactggttg atgttccagt aatagctgtt ccaacttctg ttggatatgg agcaaatttt
    36961 ggaggattag cagcattact agctatgtta aatagctgtg catctggaat atctgttgta
    37021 aatattgata atggatttgg agcaggatat ttagcagcaa tgataaataa attgtaaata
    37081 cattgatata aattaaacaa aattatataa taaaatttaa tttatattat atttttacta
    37141 ttaaaattga ggagacatat atgagtgaaa aaatacttta ttttgatata ataagcggta
    37201 tatcagggga tatgaccctt gcaagcttac taaatttagg tgtacctaag gaaatttttt
    37261 tggaggaaat tcataaacta agaatgagcg atgaatttaa tataaatatc agctcgaaaa
    37321 ctgaaaatgg aatagtaggt acaaaagtag aagtaattac aaaagaaaaa catacgcata
    37381 gaaatttagt ggatatattt gaggttatag ataaaagcac tctaaacgaa aatgtaaaat
    37441 ttaaagcaaa aaaaatattt atggtaattg gagaagcaga agcaaaagtt catggaacta
    37501 caatagacaa gatacatttt catgaggtag gggctataga ttcaattgta gatatagtag
    37561 gagcttctat attagttgac ttactttgtg tagataaagt ttgtgctagt acagtgtctg
    37621 ttggttcagg atttgtaaaa tgtgaacatg gaattatacc tatacctgca cctgcaacta
    37681 tagaaatttt aaaaggtgtg ccaattaaat taaataatgt aaatggagaa tgtacaactc
    37741 caacaggagc tgcaatcata aaagtcttgt gtgatgagtt tgtagatgag tttgaatttt
    37801 ctccaaagca aattggatat ggtataggtc ataagaagtt tgaagtgcca aatatactca
    37861 gaactgtatt aggtgaaaaa aaaaagaaga attaatttat gaaattagtg caaacattga
    37921 tgatatgtct tcagaaatct attcttattt gtatgaaaaa atattagatg agaaagcatt
    37981 agatatttat acagagagta tttatatgaa gaagaataga ccagcaacaa aagtatcaat
    38041 tttatgtaat gaatcagatt tagaaaaatt tattaaaata ttacttttag aaactagtac
    38101 ttttggagtg agatataatg catataatag agaaattcta gagagaaaat ttgtaaaatt
    38161 agatacaaaa tatggagtgg ttacagtaaa actagggtat tataaaggaa aactagtcaa
    38221 ggctactcca gaatataatg aatgtaaaac tatagcaaaa aatgaagata ttcctataaa
    38281 aaaagtgttt gctgatataa attttagaat aaaagaagaa tttagtgaaa acttattgac
    38341 ataagtaata aattttgata aattaatatt cagccttatt aaataaaagt aaacagaaaa
    38401 aaatatataa aatataaact cactcgtata tgctcagaaa tatggtctga gagtctctac
    38461 caagataccg taaatatctt gactatgagt gaaattatta taccaaaaga cctagttaag
    38521 taaatgtgta ggctgagtta aactccctat gtgttggcta taagataaag gtattttgga
    38581 agcctgtata gtaatttcac ttttgctaga aattatggaa gttactatac aggttttttg
    38641 tgtaaaaaga tttaatataa tttagactgt attgtgttaa aataaatata taaaaactat
    38701 attatacaag gagagatgta aatttatgaa acatgaatta gtacttgtaa tagattttgg
    38761 aggacaatat aatcagttga tagctagaag agttagagaa aacaacgtat attgtgaaat
    38821 acttccatgt actgcaagca tagaaagaat aaaagaaaaa aatcctaaag ggataatatt
    38881 tacaggcggt ccaaatagtg cgtatttaga agattctcca acaatatcaa aagaaatatt
    38941 tgaactagga gtaccaatac ttggaatatg ctatggtatt caaataatgt ctcatgtatt
    39001 gggtggagtt gtaagaaaag gtaataaaca agaaaaagaa tatggaaaaa cagctataac
    39061 ttatggaaaa tcatcattat ttgaaggaat aactacaaat agtgtatgga tgagtcatac
    39121 agatttaata gaaaaagttc cagaaggatt tactatagtt gcaaatacta atgattgtcc
    39181 agtagcagct atggaaaatg tagaacgaaa cttatatgga gttcaattcc atccagaggt
    39241 agaacactgt ttggaagggg acaagatact tacaaacttc ttatataata tttgtaaagt
    39301 aaaaggtgac tggactacag attcattcat agaagataaa ataaaagaat taaaagaaaa
    39361 aataggcgat aaaaaagctt tatgtgcttt aagtggagga gttgattctt ctgttgctgc
    39421 agttcttatt cataaagcaa ttggggataa tttaacttgt atatttgttg accatggttt
    39481 acttagaaaa aatgaaggaa atgatgtaga aagaatattt agagagaaat ttgatataaa
    39541 tttaataaga gttaatgcag aagatagatt cttatctaaa ttaaaaggag tttctgaacc
    39601 agaagctaag agaaaaataa taggtgaaga atttataaga gtatttgaag aagaatcaaa
    39661 taaattagga aaaatggatt tcttagtaca aggaacaatc tatccagatg ttatagaaag
    39721 tggacatgga aatgctgcta ctataaagtc tcatcataat gttggtggaa tcccagaaga
    39781 tgtagacttc caagaaattg tagaaccatt aagagagtta tttaaggatg aagttagaaa
    39841 gataggatta gagcttggta tagaagaagg tttaatattt agacatcctt tcccaggacc
    39901 aggtctagga ataagagtta taggtgatgt aactaaagaa aaatgtgata tattaagaga
    39961 agctgatgct gtttatatgg acgagcttag aaaagcaggt ttatacagag aaatatggca
    40021 agcttttgca acattaccag atgttaagac tgttggagtt atgggtgatg aaagaacata
    40081 tgcttattta gttggtttaa gagcagttac ttcttctgat ggaatgactt ctgactggta
    40141 taagatgcct tatgatgtat tagaaagaat ttctaataga ataataaatg aagttgatgg
    40201 agtaaataga gtagtttatg atataacttc taaacctcct ggaactattg aatgggaata
    40261 aaatatagaa tgccaaaaat ccacataaat acaggatttg aggtctctct tacttctatg
    40321 ttatactggt ttgatactat caatatttgc cagtatctta tggattaata agcaaaaaac
    40381 atcctgctga atgataaatt cagcggggtg tttttatttt gttttagaac tcctttgctc
    40441 tgaaactcga agtatttaac actgatgatt ttgttatctt ttagttcctt ttgtgtcatg
    40501 ctctgaaaga caaattgata caataaaaaa gtaatttctt aatcattgat ggagatatgt
    40561 gtttatgtta ctcttgatgg taatttgagt aatattgttc atagcttttt atattcactt
    40621 aattagaaaa ccatttggta taatcataaa taaacttgat gatataatag aaaagattta
    40681 taattattag aagtatataa aagtatttta tgtctatgaa atgtatgtct ataaaacata
    40741 tagatgactt ttgtatatta atgtatgaat attgatatta tagaagaatt gagttgtcaa
    40801 tgatactaaa tcaaatattt atagaagctg gagggattat gagaaataaa ataattatag
    40861 gaattacttt ttcacttgta ttactaatgt ctggatgtgc ttcaaaacaa accgcagaaa
    40921 taaacgtatt agcaactact gatttacatg gagttatacc ttatgagtta acttcttatg
    40981 taaaagaaga aagaaaaaaa gataaaaatc taactttagt agatgcagga gatttttttg
    41041 atagtggaga agtgtttggt tctagcatgg atagttattt tagtgctaga tataaagatg
    41101 gtgataataa cactgaaagt tatattgaga cgcctatttc aaaagatatg aaagaggtag
    41161 gttatgatgt tgcagttctt gggaatcatg aatttatatc caacaataaa ttttatctag
    41221 ataatatgat atctgatttt gagaagcaaa aaataaatat tttatcggca aatacgtata
    41281 aaaaaaatgg agatagttat acaaaaccat acataataaa aaatattaat acacctgaag
    41341 gaaacgtaaa actaggaatt ttagggctta ctataaaaga agttggtgag aggaaacagt
    41401 ggaaagatgg aaaacttgtt gactctaagt ctttagaact aaaagaccaa gagggttaca
    41461 atggtgaatt gtatatgaat gatttagtag aagatgctaa aaagtgggtt ggtataatgg
    41521 aaaaagagaa gcctgacatt atagttgctg tggcacatac aggtgagaaa cctaagaaac
    41581 ctaaaaaccc aggaaataga atacaagact tagctcaaca agttgatgga attgatgcta
    41641 tagttgcagg tcataatcat gttcaaattg agcaacatga ttataaaaat aaatcaggag
    41701 aaaatgttat agttactgag ccaggaaagc atggggaatg tatttctaag attaatttta
    41761 aattagaaaa aaacaatgat aaatggaata tagttgacaa atctagtaaa ttaattaaat
    41821 ttgaaaaaaa tcaagaaagt gataaatttg gagaatttat ttcttcaatt aataaagtaa
    41881 gtaaagataa gaaagaaata agattatcag aagtaaagac tcctgaatgg gataaaatat
    41941 atgtttttaa atctggaaca tctgcagatg atatatataa aacaatagga tataaatgga
    42001 gaaatatatc atctccaaag ggtgatgaca tggtgcagat tgttgtaatg aataataata
    42061 agcctgttag ctatgtatat ggtgaagctg aagttatgcc aattaatata aaatgtgatg
    42121 aatcagagta taaagataat gttgtaacta tttatcctaa taaaaatgat aaatttaaaa
    42181 ttgaaaaagg taaacaagaa tatagtatta atttaaaaca tatacaatag aaacttttat
    42241 cttatttaag tttagattgt acttaaaata aagtgtgtat tattttaaaa attgatgata
    42301 attaaaatca caacattaag gaatagtaca cactttttat gctaaaaaat agaatgttaa
    42361 aaaattaaac ctttaaatta aagtacattg cttatattat ccaatattat tttcaaaggt
    42421 tgtgtaatat ttttatcctt atgtaatatt gctaacattt tattttccat atctatccca
    42481 tctaattcaa tcaatcgcaa ttttttatgt aagagtgaat ctaataataa attttctggc
    42541 aaaatggtta tacctaaccc tgcttgtgca gctttaataa gtgcaaaaga attaacactt
    42601 tcccaaacag gataagcttt ttggtttgaa agagataata cactgtccaa agtattgcga
    42661 atggcacttc cttgctcacg taacaataac ggaaacgtgc ataactgtcg tagagataag
    42721 gcttgtttag tattggtggt ggttatgcaa tcatactttt aatccaagag aaagtagtaa
    42781 atcaaactgg atggataagt gagaaaatgt ttacagatat tataactatt tcacaaatga
    42841 caccagggcc actggctgtt aatacttcta cttttgttgg acttcaaatt ggagggtttt
    42901 ggggagcagt tgcagccact ttaggttgtg tattatgtgg tgtagtaata tctctatttt
    42961 tatataattt ttttcaaaag catcagaact ctacttatat atttgaaata ttaaatggtc
    43021 taaaatcagc ttcattaggt ttaattatct cagctgcggc tacaattata atccttgcat
    43081 tatttgggac gaataaagtg tacttcaatt ctatatttga gatgctaaac tggactgcac
    43141 taataatctt ttttgttaca ctactgatag tacgtaagtg gaaaattaat cctattgtga
    43201 ttataataat aagtggtata ctgggaatat ttttttattt ataagttttt ggtaagtaga
    43261 ggatatctat ttttttatat cttaaatcca aatagccaat aaagtaaggc catttggatt
    43321 tttaataaat tctgaaaaga taatcagaaa cccatataac atttaaatta tatcactggt
    43381 tttctacaaa ccaatcataa gcttcttggc aaaatttctc actatctata tagtaactat
    43441 tatgaacatt attaatttca acaagtttct ttttacattt tagtgaatta taatagctat
    43501 caaaatattg ggttttaaaa tatctatcat gaagtgtttt aaagacaaga gtaggtatgt
    43561 tgactccatc ttttatctta ctatcttttg caataaagca ttctaacatt gtattaatta
    43621 tctttgtact atttttccaa atatgaggat ttttcttttc aaatatttta taaaattcat
    43681 tatcatagac ttcattataa cctttatatt taggtggttt tactgttatg tgaaaattag
    43741 gaagaaactt aaagaaaaag tttaataatt taacagtgag ataggtatag cctactcctg
    43801 ttgtgttttt ataatcaaca caagcaaact gagcaaaact ttttaactta ttgtctgaac
    43861 atgcataata ttgtgcaaac ataccaccaa ttcccgtatt cccatataga tgtatgttgg
    43921 aagaataatt tttttctatg tatgtaatga cagaatctaa gtcttttaca attgaattgc
    43981 gagaaaaatc tttttcatat ccatcacttt tccctgttcc tgaaaaatca aaagcaaata
    44041 tatttacatt atgtttggca agtggttcat atgctttttg catctcaagt gtagtgacaa
    44101 taggagtatg tgtaacaaca atattcatgg atgattttct ggtaataaat gagttacaaa
    44161 aaatcattgt atctccattt ggtattttta tatgtttcca actcatttga ttttcctttc
    44221 tctattataa aatataataa tttttatgtt ctgttttata tattattcat attttatcac
    44281 agataatata ctaaagttaa tttttttatt gtacacctat tataataatt cttctataat
    44341 ataagacatt ctataatttt atatttaagt tcatgtgtat ttaattgttt ttatataaaa
    44401 tgtccagtaa tactatttgg atttattttt atttcatcag gagttccaga agcaacaatt
    44461 tcccctccat ttattccact gtctggacct atatcgataa tccaatcaga agcacgtata
    44521 acctctgtat tgtgttcaat aattaaaagt gaatgctgtt catttacaag ttttctaaaa
    44581 gtaattaaca aattttctat atctttaaaa tgaagtccag tagtaggctc atccaatatg
    44641 tacagcattc gttttttagt ttttgtccct aattcttttg ctaattttat cctttgagat
    44701 tctcctccac ttaatcctaa tgtattttgt ccaagcttta gatatccaag tcccacttca
    44761 tctaataagg agagttttct atatatatca gagcaatcgc taaaaaatgt ttttgcttct
    44821 gctacttcca tgtcaagtat atctgcaatt gtctttccct tgtattgtat agacagtatt
    44881 tctttttgat atctcttacc ttgacattta ctacaagtta cccattggtc tgccatatat
    44941 tgcagaataa tttttgtttt acctaatcct tgacagtttg ggcattgccc ctttttgcta
    45001 ttaaagctaa aatagctttc atccaataat ttttgttttg cttgtggagt ttcagcaaat
    45061 agagaacgaa tttcatcaaa aatattgata tatgtagcag gtgtagaacg gctagatttt
    45121 ccaataggtg tttggtccat taagataaaa tcatcaatag catcaaatcc agtaaattca
    45181 gtataatttt tatctgggat agatttttgt ttcaattttt cttctagagc aggaagtaaa
    45241 ctgtgaaata caagagagct cttaccagaa ccactaacac ctgtaataga gcacatacag
    45301 tttaagggaa tttctacatc aatatttttt aagttatttg catggcatcc ttttaagctt
    45361 aaccatttac ttgggataat tttattttgt gaacctgttt tattgtatgt acttaaatat
    45421 ttacctgtta tagagtttgg attttttata atttcttggg ttgtaccttc tgcaataatg
    45481 aacccccctt ttgtaccagc acttgggcct acatcaataa tataatcagc agacagtatt
    45541 gtatctttat cgtgttccac aactataaca gtattaccct tatctcttag ctcttctatc
    45601 attttgatga ttaaattatg gtctcttggg tgtaaaccaa tagatggttc atctaaaatg
    45661 taggttaagc ctactaattc acttcctagc tgacttgata atcgtactct ctgcagttct
    45721 cctccagata aagttggagc tgttctatct gttgatatat agctaagacc tacctttaat
    45781 agataactaa ctcgaatttt taattctgtt aagatatcat ttaccaaaga gagctcattt
    45841 ttttgaagtt gatttggtaa ttcacacaac caattccata actgttctat cgtcattttt
    45901 gttagttcag ggaagcgata acctttgatt gtagtgaatc tagcctcaat gcacagcaat
    45961 tcgccaccac atgtattgca aggaattttg ttcatatatt gatgcaaggt attattttca
    46021 gaactagatt ctctaaaaag tctttgaatg tggttgattg ctcctgaagc aggtcttcta
    46081 atctttgatt ctcgtccctt tgattcaaag ctaaattcat atatcttatc gtctgtacca
    46141 tataagattg catctataaa atctcttggt aattcattcc aaggtatatc taggtctata
    46201 tttttatctt ttgctattgc atataattca cctaccatcc aattaccatt tggctttttc
    46261 cccctcaatt ttccaaaata gtaagtggct ccatctaata ttgaaatagt tgggtctttt
    46321 acaatcaatt gaggattaac ttgataggta aaacctaatc cattacaatc gtgacaagct
    46381 ccagtatggg agttagcatt gaaggttgaa gtatttatct tagaaaaaat agtttgacaa
    46441 tgtggacaat aattttttgt attattcttt ttttctaatg gaattttgca atatggacag
    46501 taactttttc cgattgatac aaataacatt cttaaataat cccaaatgct tgtaagagta
    46561 ccaacagtag agcgaggatt ttggcttcct tttttctgtt cgatagctat agtaggggtt
    46621 aatccctgaa gtaaatcaaa ttccggtttt tgaagctgtt ctgaaagttt tgctccatta
    46681 cttagatttt caaaataacg tctttttcct tcttcataaa tcgtatcgaa agctaaagaa
    46741 gattttccac taccacttac acctgtaata acagttattt gattttttgg aattgtaacc
    46801 tcaacatcct ttaaattgtt ttgccttcct ccttttaaat agatagagct tttattatga
    46861 caaagtgaat ttttaattga tttatttatt gattttgtaa atttgaattt cttttcgagt
    46921 tcaagaggat tgcctaattc ttttatttcc gaaatgccta atatttttaa ctgagaaaag
    46981 attgatttca aataatcttc aggcagtttt tctattaaca aattaaggtc ttctattttt
    47041 atatttccga ttgataatat ttctgccatt tcatctaata aatttttttc tgctcttgtt
    47101 aattgattgt attttttgga acttaattct gtccataaat cccatggaag caattctaaa
    47161 ccaatcatat tattaaagtc atgtagaaga ttacccttta cagaatgcca acctttccaa
    47221 tttttattat atccaaagtc atctggatga gcatgtcctt gccgacatct tatccaggct
    47281 tctgaaccag ttaaaaaacc atctgttttt ccgacatcat gagtgtcaaa attaatttga
    47341 aaataatttt tttgtatgtc atctatttgt gcatctattc ttatccatct ctttgttaga
    47401 gtatcatagt attctacaag ccagtggtct tcataagtaa gctcactttc aaaataatta
    47461 gcaaaacctg ctctcattct agcctcaaaa cctcgatatc ttaggagcga tacaagaagt
    47521 aaagaataat ctctgcacat tccaacaagc ctttgttttg ggtttcttgg aatagttagt
    47581 gggttagaat ttatttcaaa tattcttttt aacatttgag gaattgtacg aagcaattcc
    47641 tcatcagatt gttgtttatt aaaggatata ccatataatt ttccttggtc tccatgaatt
    47701 actaggcttt gaaccacttt taataactca attggagtct ttggtaaatc ttgatattta
    47761 ttttcaaatt ttttaggatt tgtgatgatg ctttgatttt tatagttatt tattttcatt
    47821 tatatttcac ctctctaaat ggatgatata ccctgacatg gtgtcatatg caagggtaat
    47881 ttaaattttt tggccaaaat ttaaaaataa ataaaaaatg atacagtgat aaattgtaat
    47941 acaaatatag tttcaaattg catataatat aattatttaa taaaaccatt atctatggaa
    48001 tgttaatgaa atgttaagat ggcattcttg aatattaaga aatagaatac tataatatag
    48061 tgtgaaggat ttttagcaaa aaaatttgta agtgttaata tacattaaaa aataggttta
    48121 taatttggag gaaaaaaatg aaattatcaa ggtataagaa aaatagttta gttctgtcta
    48181 taattttatg ttcacttatt atttcaatcc ttgtaattac tgtttttaat accgtaaaac
    48241 tacacaatat atctaataaa caatctaaaa actatttaaa tgatgtatct acacagattg
    48301 taatgaatgt tgattctaaa atcaaattta ttttatccga cttaagaata atggcagatt
    48361 ttattaagca atatgaaggt gatagtcgat atgagtattt atctaaaaga aagactagtt
    48421 ataagtatta tgatgttgga ataatagatt tagaaggtaa agctgagttt ttaagtggaa
    48481 aaaagtttga cttaaagaat actgtttcct ataaaaaagg aattgaaggt aaagaatact
    48541 cagaagtaat agagtcaatg ggttttgttc tttatagtgt acctatattt aatgataatc
    48601 atgaagtatc tagtatttta ataggtgtat ccaataaaga aagtatgaat gagattctta
    48661 atatagataa ctttaatgga gaaggaacta ttgaaattat taatagtaag ggaaaacctc
    48721 ttttcatagg aaaaaatact gaattaataa aagaccttag ccataaatat aaggattcta
    48781 cagatgaacc ttgggcacaa aagatgctta atgattttaa aaatgataaa tcagggaata
    48841 ttacaataac ctcatcaaaa ggtgtacaat gcttattgac ttatcatcca attacaagag
    48901 gtttaaatga ttggcatttt cttctcatag tacctgaaga tgctgtactt ggtgaactta
    48961 ataagttaaa ttcttttaca ataacaatga catttataat tacatgtatt attggaatta
    49021 taacattaat tttatatatt ataagaagaa aatatgtaaa tcaaatagaa aatattgcat
    49081 atttagatag tataacaaat ggcataaatt caactaagtt tagtatgcta gtcaaacctt
    49141 taatttcaga atcacctgat agtacttata tgatgatagc aatgaatata aaagatttta
    49201 agctaataaa tgattgtttc ggtagtgaaa agggaaatat gacactaaaa catctttaca
    49261 atattttgta taaaaacatt gatgaaaatg aagaatttgt gtgccgtcat gatgcagacc
    49321 ttttctattt gcttattaag aatagaccag tgttagaagc cctggagtta ttatataaaa
    49381 tagaagctga cacaaattat tttaataaaa atagggaaaa tccatacttt ttaagactat
    49441 cagtaggtat ttacacaatt gaaaatcata atgaagattt aattacaata caagaccatg
    49501 caaatacagc aagaaaaagc tttaataaaa ctcatcaaag tgatttttct ttttatagtg
    49561 atatagaaag aaaacgatta atatctgaaa aggaaatttc aaatttaatg gaaaaagccc
    49621 ttgaaaataa agaatttttt atgtgcttac aacccaaaat tgatataaaa agtggaaaaa
    49681 ttagtggagc agagtctctt gtgcgttgga aaaattcaga aaaaggcata atttatccaa
    49741 gtgattttat tcctcttttc gaaaaaagtg gatttatatg taagttagat ttatatatac
    49801 tagaagaaac atgcaaattg atttctaagt ggataaaaga aggtaaagaa gtgttgagaa
    49861 tttctgttaa tgtatcacgt caacatctga atgataaatt gtttttagaa aaatataagc
    49921 atatatgcaa taaatacaat gtaccaacat gtctgataga tttagaactt actgaatcta
    49981 ttttcttaga aaatccagaa gctatagata taatagagga tattcattct aatggattta
    50041 aatgttctat agacgatttt ggatttgggt actcatcatt agggatatta aaggatttta
    50101 aagttgatat aattaagtta gaccgttcat tctttgttag caaaaataat atagaccgtg
    50161 gtaaggtagt tattaagtct attattgaac ttagcaaaag gctaggtatg aaagtaactg
    50221 ctgaaggaat tgaagaatta gaacaagttg agtttctaaa aaatgtggga tgtgattata
    50281 ttcaaggtta tgttttttca aaaccattat taatttctga ctttgaaaat tttgcatatg
    50341 aaaataataa aattaaaaat atccattatt aaatgtacaa gataaaaaat ttaggcaagt
    50401 tatataatta aaaatataac ttgtcttttt tatttatata tttaataaaa atatatttgc
    50461 agattttgta aaaataattt tcaaatgttg taagttgtgg ctaaagcttt attgtattat
    50521 tctgaatata gaaagatagt tactgtctta gataaaaact gaaacataca taataatata
    50581 aaatttgcta tattaataac tctaattatt ttggaaggag ggaaaatatg ataagtggga
    50641 gaatgttact catcatgaat tttctaaaag gaaaaaataa gacaagttac aaggatatta
    50701 gcaaacaact tagtatagaa gaaagaaaag ttagatatga tataaataat ttaaatattg
    50761 ttttgcgttc attaaacaag tcagtaatac aaaagatgaa caaaggtgtg ttaattattc
    50821 ctgatgactt ccaagtagta tgtataaata ataacgaata tgtattttcc ttaactgagc
    50881 gagtatctat tatgaaaatt atatcaatgt ttaaaataga taatctaaac ttagaaaaat
    50941 taagtaaaga atttcaggtt tctagaagtt caattaaaaa tgaccttaat gttttgtcaa
    51001 gtgaattaga aagaaaccaa ataactatta cttatgataa atcttttaaa ataagtggag
    51061 aagaagacat tatatataag caaagattga acatattaaa atcgtattct tatctttttg
    51121 gtaaggaaaa atctgattta agtgtatttg agaagtattt agtgaatgta attgaaaaaa
    51181 tattcaaagg gatttatttg attgatatat ataattggtc tatgaattta ttattgcaaa
    51241 tgggatggac tttaaatgat gaatctttta catggtatgt gtcaaacatt ttccttttta
    51301 catggtatat acattcagat gaaaaacatc ctttagaatc tgcttcatta agtgagccgt
    51361 gttttgaaaa taagtttgac caagaatttg aaacaattat tcaaaaaaag ttgactaagg
    51421 aacaaatttt tctgttaaag agttttgtat tttttacaaa taaatatgca agtttaaacg
    51481 aggatatgga tttgattagt acagaaatta tagttcaaga cctaatttac aatatgtcag
    51541 aaatattaga gattagcttt aaagaagata tgattcttta taaaggatta ctcaatcata
    51601 ttgccccatt gatagagaga atcaaacaga atatacagat ttatgaggaa ttacttcatg
    51661 taataccaga taaatatcaa tatgtacttg aagctactaa ggtttctatt caaaaaaaca
    51721 gagttttaag tcaaataaag aatgaaaatg aaacaatact tttggcaata catttcttag
    51781 gaagtataca tagaaatgag aggaataact acaaaaatat attgttagtt tgtggatttg
    51841 gatatggtgt aattgctatg ttaaaggata gtttagtaaa tgattaccag ataaatatag
    51901 tagagagtat tccatcatat aagttacaag attataaaga atgggaaagt attgacttca
    51961 ttataacaac atcaaagata gtaatagact tgcctaaaac aattattgag ataaatccat
    52021 ttttacaaga agaggattat ttgaagtttg aagaaaaagg gataaaaaga aagaacgtct
    52081 taactaatta tatgtcaata aaaaaaaggt tggacttttt agaagaagat gcccaaaaaa
    52141 gagtattaag tattatatgt caagaacttg gatacagtga tgagagaata ttatttagac
    52201 aacttaattt aagtgattta ataggaattg ataccataag gataattgat ggagatatga
    52261 aatgggaaga tgctgtaaaa attagttcaa acatacttgc taattgtgat tttgtatcag
    52321 cacaatatgc tgagaatatt atagatattt taaaaaatgt gggtttttat gctgtaaaag
    52381 ataacgaatt tgctttacta catggcaatg atagttcatt agtcaaagta agttcgataa
    52441 gtttattaat atgtaaagaa gcagttagtt ttggagataa aaaggttaaa atcatatttt
    52501 gcctagcaag cagagataaa aaagagcaaa ttcctgcaat aattaatcta actaaaatgg
    52561 tttataagac taattttatt gctatgttag aaagtgcaaa gacaaaagaa gaagcagaag
    52621 ttttaattca taaatatgag aaggaggtca ctgtatgaat aaattagtta atgtaaaatg
    52681 ttcatttaaa atacatgtta attcaaaaga agaagccatt atatcgttag ttgatgtagt
    52741 gtcaaaagaa ggatatttaa tagataaaaa ccaattttta aaagatgttt taaagagaga
    52801 agaaacattg tcgacttata ttggacatgg tataggtctt cctcatagtc aaagtgtagg
    52861 tgttaaaaac tcttgtatta caataggaaa gcttgatact cctattgaat ggacagaaga
    52921 aggagaaaaa gtggatttaa tatttttgat atcagtaaca aaagacaatg aaaataatct
    52981 tcatttaaag attttatcaa aattagcacg tttattgatg catgaaagtt ttagaaatca
    53041 aattagatgt tcagatgaac aaactgttta taatcttata aaagaaaaaa tagaggagga
    53101 agattaaaat gagaatttta aagtttttgc aaaaacatct tatgacagca acatcttata
    53161 tgattccatt tgtggttgca ggaggaatat tatttgcttt atcagtaact ttaagtggac
    53221 aagctgctgt tcctgaaaca ggctggttgg ctaaactgaa tcaaattgga gctgctggct
    53281 tggctttatt cattcctata ttaggtggat atatagcatt tagtatggca gataaaccag
    53341 gattagctcc aggtatgata ggtgcatatt tagcaaaaga agtaaatgca ggttttatag
    53401 gtggtatcat agcaggattt atagcaggat ttgttgtttt acaattaaag aaaattaaat
    53461 tagctcctac aatgagaact ttaggttcta tatttattta tcctttgatt ggtacattga
    53521 ttacaggtgg tataattgta tttttaattg gagaacctat agcttctttt atgacatgga
    53581 tgacaaactg gttaaatgga atgtctggtg tttctaaaat tcctttaggt ggaatcttag
    53641 gaggaatgat agcctcagat atgggtggtc ctataaataa ggttgctgct acttttgccc
    53701 aaactcaagt tgacacactt ccatatttaa tgggtggagt tggcgttgca atttgtatac
    53761 ctccaattgg tttaggatta gctacattat tgtttccaaa aaaatttagc aaggaagaaa
    53821 gagattcagg taaagcttca ctattaatgg gatgtgttgg aattacagaa ggagctattc
    53881 catttgcaac atcagatcca gttagagtaa taccttgtat tatggctggt tcgattatag
    53941 gaaatattat ggctttttta ttaggatgtt taaatcatgc accctgggga ggcctgatag
    54001 tattacctgt agttgacaat aggttggggt atattgcatc agtattaact ggagcagtag
    54061 ttgttgcagt attaatgaaa ttagtaaaaa aagacgtgaa agaagatgaa gaaattgaag
    54121 aagatttaga tgaatcaatt gaattaatat ttgaagaatt ataaatataa atggaaggaa
    54181 tttgtataaa aaagtaaata taaaactata aatttaaagg agataagaat atgaaaattg
    54241 tagcagtaac atcttgtcct agtggagtag cacatactta catgtcggct gaatcacttg
    54301 aattatcagc caaaaaattt ggtatagaaa taaaggttga gactcaagga agctctggaa
    54361 tagataatga attgtcactt aaagatattg atgaagcaac ttgtgtaata ttgactaatg
    54421 atgttgagat tagaaatatg gaacgtttta agggaaagaa ggtagtaaga atgagtgtat
    54481 ctgagataat taagaaatct gatgcattaa ttaagaaaat aaaagatact tttcaataaa
    54541 aattgattta ttttacatat aactcaaaga tgtttttaaa gaaattttta ttaaaagcat
    54601 ctttgaaaat atcttaaaaa tagaataaaa ggagatatag tcatgaaaaa gttaccaata
    54661 aagactgtcg tacaaagatt actaaactta caagaagagg ggaaaagtgc aactctttta
    54721 ggaatcggtc ctatgtcacc aaacttgtta caagcaagtt ttgaactagc aaaagatgat
    54781 gattttcctt taatgtttat cgcaagtaga aatcaagtag atgcagatga actaggtggc
    54841 ggttatgtaa atggttggaa tcaggaaatt tttacaaaag atattaagaa ggttgccgac
    54901 aaagttggat ttgatggttt atattattta tgtagagacc atggtgggcc atggcaaaga
    54961 gataaagaga gaaatgacca tttgcctgtt gatgaagcaa tggagttagg aaaaaaatct
    55021 tatttagcgg acattgaagc aggttttgac ctattgatga ttgaccctac aaaagaccca
    55081 tttgaaattg gaaaagtaat tccactagat gtagtactag aaagaactgt agaattaatt
    55141 gagtattgtg aaaacgagag aaaaagactt aatctaccag acataggtta tgaggttggt
    55201 acagaggaaa ccaatggagg tctgacttca actgatacgt atgaaacatt tataactaga
    55261 ctaaaggttg aactcgataa tagaggtttg ccaatgccaa catttatagt tggtcaaact
    55321 ggtactttaa ctagaaagac agaacaagta ggaactttta attttagaaa tgcttatgat
    55381 ttagctcaaa tggctaagaa atatggagtt ggtttaaaag agcataatgg cgattactta
    55441 gatgatgtaa ctttattaga acatatacca tctcaaatta ttgctacaaa tgtagctcct
    55501 caatatggta ctgaagaaac tagggcatat ttaaaattag cagaagttga gtgtaaatta
    55561 gaaaaagagg gcttagttga gaaaacttct aatatcagac atgttcttct tgttaatgca
    55621 attgaatgtg gacgttggag aaaatgggta gtaggagaac aaaaaaatct aactacggaa
    55681 gagatattta aggatgaagt tttatctaat gaaatactag atattgcagg acattataca
    55741 tttaataatg atgatgttaa aaaagaaata gaagtgcttt atgataattt aagtaagaat
    55801 aatattgatg gtcaaagatt tgttgtagac catattaaac gtcctttgcg tgattatgca
    55861 gaatgctata acttaaaagg tgttactacg aggattttaa ataaataata ggacttatag
    55921 tggtgagtat tgttatacta ggtatttata gttataatta tacttaccac tattaaattt
    55981 aggaggacta atattttgat caatttaatt tttgatgtag atgatacttt atataatcaa
    56041 ttaacgcctt tttacactgc atataataaa gtgttttctt caataaagga tatttctatt
    56101 gaagatttat atatgagtag tagaaagtat agtgatgagg tatttcatat gactgaaaat
    56161 ggagaaatgc caatcaaaga aatgcatatt tataggataa tgaaagcttt tgaggagttg
    56221 ggaaattcta ttacagaaaa agatgcacaa agttttcaag atgagtatat atatcaacaa
    56281 tcacaaatta cactaatacc agaagttgag aggattttaa atttttctaa agaaagaaat
    56341 attaatctag gaattataac taatggccca tcaaaccatc aaagaatgaa gttaaagcag
    56401 ttaaatattg aaaactgggt tgataaaagt aatattttta tttctagtga ggttggattt
    56461 tctaagccag atactaatat ttttagagta gctgagaatg ttatgaattt agatagagaa
    56521 aatacatatt atgtgggtga ttcttataga aatgatgtat taggtgctaa aaaagctggt
    56581 tggaagagta tatggttgaa ccatagagga catgaggtag aagagttgtt ttataaacct
    56641 gattttgtaa ttttggaaca taaggatttg atatctttat ttataaaaat atgtagttat
    56701 aaaaatatgt agtaaaaaaa tgttaataat agaattgtat aaaataatta ataataacta
    56761 tataaaatta tatgtttaga ttgtgagcaa tctattttat gttaaaaata gattatttag
    56821 aagaagggga aatcttaaaa aaatttgaat atcccctttt tattatttaa ttaaaatgaa
    56881 atagttagaa atatatttta tttagtttag tcaataacat tttgggattc tttataacta
    56941 catgttaact gttgaatagg tttatcatat ggtttaaccc atcctttatt aatacttaat
    57001 tctgtaagtg ctgtgtgacc ttctaccatt tgagttaatg aagctgaata tatagctctt
    57061 aattcaggtg tactacttgt caatgcagaa ttaagataca tattggcaga tgctttagca
    57121 gatgaaagca tacttaacat tattacttta tcgtctatat aagtattgtt cttaattaga
    57181 ttttctatca tatttctcat gttatctata cctcctcaga aattagtatt tcattttcaa
    57241 ctataaattg ttgtatagct ttaattcttc cttctgttgc taatatacta gattctgatt
    57301 gtctttttag atcttcatct gatattaaca tatttattgc tctttgcata agtaatccgt
    57361 cagactccat ttttataatt gctgctaaag atattttttc agtttctgaa agtattctca
    57421 tttaatcatc tcctatattg taatttatgc tttaatagta tgtctttctt ttcaaggaaa
    57481 taaccattac acttttttac agtattttat attctcgtta tatcaaaatt tagtcgactt
    57541 aatatattaa tataattttg taggatattt atcattaaat atctataaaa taaattgttt
    57601 gaatattcat aaagtaaaat ttatttgtat gtacttcttc aagagcaaat atgagttttt
    57661 tcaaaaaatt aatttttatg ataattatta agggctatat ttttcttaaa gttgtgatta
    57721 tcattattaa tttacaataa aattgccgaa aagccctctc ctttaggtgt agggatgaaa
    57781 ggtaaggtct taatataaaa aaagttagaa taatgcttgt aatctaatac cgctactgat
    57841 accctatatt taggaggtgt tagtttttat gtataaatct aacaaaaata tagtttatga
    57901 ttgcaaatat catattgtat tttgccctaa gtatcgtaaa cctgttttag ttggcgaagt
    57961 tgaaaagatg ttaaaagaaa tactaccata taaagcagat gaacttggag cagaaataat
    58021 agaaatggaa actgataaag aacatgtgca cttattaata tcttgtgacc ctcaatatgg
    58081 aattcataaa gtagtaaaag gtttaaaagg ctttagttcg agagtattaa gagagaactt
    58141 tccacattta aagtctagta tgcctagcat gtggactaat agctattttg taggtactgt
    58201 tggttctgtg agtttagaag tagttaaaca atatatagaa aatcaacaag ttaggacaaa
    58261 gaaatgataa aaactcatag agtgaaatta aacctaacta gaacacaatt tgaattagta
    58321 agggaaaagc aaatggaaag tgctaactgt tggaactaca tagttaactt atctaaagaa
    58381 tactattttg aacataaaca atggattaaa aagaatgata tacaaaagtt aatcaaaggt
    58441 aagtacaatc ttcatagtca aactattcaa gctatatcag ataaatttga tgcaaataga
    58501 aagactatat cagaattaag aaaaaaaggt aatactaaag caaaatatcc atacaagact
    58561 aagaaatttt atataatacc ttttaaagct agtgctatta atagaaatgc taaaggcaac
    58621 ttaaaattat caatgtcaaa aggcaggtat ttagaattag attttaatgt tgagaatata
    58681 aaaactgctg aaatagtatg gcgaaatgga tattatttat actacacttt tgacaatgaa
    58741 ttaaatggag ttgtagccaa aggcactaat accgtaggtg tagacttagg ggaaatacat
    58801 tcgatagcta gtgtaacaaa taaaggtgta gggcttattc tatctaatag agaaggcaga
    58861 agtattaaac aatttagaaa taagatgtat gcttatatat ctaaaagatt aagtaaatgt
    58921 aaaaaaggta gtagacaatc aaagaagtta tggagattaa aaaataagat aagaagtaaa
    58981 acagataatc agttaatgaa tttatatcat caaacaacta gaaagtttat agacttttgt
    59041 gtagagcaga aggtatgtga aatagttctt ggggatataa aaggggttga aaaagatact
    59101 aagaaaaaga aaagattaaa cagagttaat agacagaaaa tatctcaaat ggagtatggt
    59161 cgaataaagg actatataaa atataaagca aaagagcaag gtattgaagt taagttagtt
    59221 aaagaaaact acacttctca aacttgccca aagtgttcta aaaaacacaa accaactggt
    59281 agaacttata gttgtacttg tggttatgaa actcatagag atatagttgg tgcttggaat
    59341 attttaaata aaaagcataa atatggttta gtagatttta gaataaatca taagcaacca
    59401 ataaatttaa aagtatctac cgtttgagcc atgtgtgggc gaataacttt aaggtagtag
    59461 tggcgaagac accgtccgaa gtagcttggc tttgtaacca cctctgttgt actgtttaag
    59521 tataaataag tgtgttagcc accaagaatc cccttgcttt agctatgggg agtgtgtcaa
    59581 taaaatactt taatgaaaaa tagctaactt taattgtagg atattcttaa tatatttgaa
    59641 ataaataaaa atattgaagg atataatatt caattggtat ataaataata aatgatagtt
    59701 aaattttacc ttagaggcta attaggtgtt tagtcaaagt taatgccttt agaatacaaa
    59761 tgtattctaa aggcattttt actatgtatt ttcatttata ttactaaaca ttatctattt
    59821 ttatatacat ttctatgtat tttattttca tcaaatcggt ctataaactc atttttttct
    59881 tcactatatc caatagatat aagtgcataa ggaactaatt ttaatggtaa gtctaaagtt
    59941 tgtctaactt tatttaccac tttattttta ggaaatactc ctgcccaaca agcagctaga
    60001 ccttgattag taacttctaa taataaattt tgtgtgcaag cacctaaatc ttgtatccat
    60061 ttaccaggtt ttaagaattt atctctattt ccaagcacta ctatacataa tggtgcaaat
    60121 tctatatgct ttgctctatg ctgtgacttt gagagctctt tcagcagttc cttatcatct
    60181 actataataa attcccaagg ttgagcattt tttgatgaag gtgcttgcat agctgatttt
    60241 agtatttcag ttattttatc ttcttctata tttttatcta tatatgttct tattgaacgt
    60301 ctatttttta taacttctaa catacagatt ctcctttaaa taaaattatt tctatttata
    60361 taatatcatt actttaaatt ttagctatta cttatataaa gaataaactt tgtgttggtt
    60421 ttatctaagt aaaagtaaca ttcagaacta aaaaacaaga ctataaatcc aaagtattaa
    60481 ttaaaaaaga agtaataaaa aataatatac aagatttaaa aaagagtatt gggcttaaaa
    60541 tttaattaga aaaccaattt tgagataatc tgttttttat tttaacaaaa aaacataaat
    60601 aaagttcgat acatagcgaa tgaaaaaatt acaagaaata ttttttttaa aaaatttcaa
    60661 aaaagagttg ataatatatg tatattaata taaaatttaa ggtgtataat tcgtataata
    60721 atacgaatga tttgtagtgt aaaataactt ataaacggag aggggaagtc taatgaaagt
    60781 agcagtagtt atgggttcaa aatcagatta tccaaaatta gaagaaggga taaaactgct
    60841 tgaaaaatat ggaatagaag ttgtagcaag agcattatca gctcatagaa caccagaaca
    60901 actttcaata ttcctaaaag aaatagaaga tgatactgat gtaataatag cagcagcagg
    60961 aaaagcagca cacttaccag gtgtaatagc ttcacaaaca ttaattcctg taataggatt
    61021 acctattaaa tcatcaacta tggatggact agactcactt ttatcaatag ttcaaatgcc
    61081 aaaaggaatc ccagttgcaa cagttacaat agacttaggt ttaaacgcag ctttattggc
    61141 attacaaata atgactttaa aatacccaaa attaaaagag gatttgaaat catatagaga
    61201 agagatggca caaaaggtgt tagaagacga caagaattta agggggtagc agaaatgtta
    61261 ttatatgaag gaaaagcaaa acaagtatat tctacagata atgaaaatga atatgttgtt
    61321 tattataaag atgatgcaac agcatttaat ggagaaaaaa aagcagaaat atcttcaaaa
    61381 ggtatactaa ataataagat atcaacaata atctttgaaa tgttaaaaga aaataatata
    61441 aatactcact ttataaaaag tttatcagat agagaaatgt tagttaaaaa agtagagatt
    61501 ttaccattag aagtaatagt aagaaatatt gctgctggtt ctatctgtaa aagagttggt
    61561 cttgaagaag gtgtagtttt cgatgagcca atttttgaaa taagctacaa aaatgatgct
    61621 tatggtgacc caatgctaaa tgatgattat gcagtggcaa tgaagttagc aactagagaa
    61681 gagttaaaat tcttaagaga agaaacttta aaaataaacg agttactaaa agcatttttc
    61741 ttaaaattaa acctaaagtt agttgacttt aaaatagaat ttggtaaaga ttctgaagga
    61801 aatataatat tggcagatga agtatcacca gacacttgta gactttggga tgtaaataca
    61861 aatgaaaagt tagataagga cagatttaga aaagaccttg gagaccttgt agaaggttat
    61921 acggaagtac tttcaagaat gaataataaa taggaggcaa atgtatgtgt ggagttttag
    61981 ggatttactc taataaagat gtaactaaag aactatatta ttctttatat tctatgcaac
    62041 acagggggca agaaagctgt ggtttagctc ttttagatga tggagaaatt aagtacaaaa
    62101 aagatatggg attagttgga gatgctttta aggaaaatga attatctaag ttaaaaggaa
    62161 atatagggat tggacatgtt agatattcaa cagctggagg aagtcatgtt tctaattgtc
    62221 aacctttagt tggaagttgt agaaaaagac aattagccat agctcataat ggaaatctag
    62281 ttaatgcaaa ttatttaaaa gatatgctag aagaagatgg atatatgttc caaactaatt
    62341 cagatacaga agtgatttta tatatacttg ctagatatta caaaggggat atagtagaaa
    62401 gtttaaaagt gacaatggat tatataaaag gtgcatatgc tcttgtaata atgagtcaag
    62461 aagaattggt tgcagttaga gacccacatg gattcagacc tcttgtactt ggtaaaaagg
    62521 gtgatgaata tatatttgca tcagaaaatt gtgcaataga tatacttggc ggagaagtta
    62581 taagagatgt agagccagga gagataatag tagtcaaaga tggagaacta aaatcatatt
    62641 tttattcaga aaattataaa ccagttaaga aaagttgtat atttgaacac atatactttg
    62701 caagaaatga tgcaactata gacaatgtaa atgcatatga atttagaatt aaatgtggtg
    62761 agagattagc tcagaatgaa actgtaaagg cagatatggt agttccagtt ccagattcag
    62821 gttggccagg agctattggt tatgcaaatg ctagtggatt aaaaataagt gaaggcttag
    62881 ttaaaaatag atatgtagga agaacattta taaaacctac acaagaagaa agagaaatag
    62941 cagtaaaaat aaaattaaat cctctttcaa caataataaa aggaaaatct ataattttag
    63001 tagacgactc aattgtaaga ggaactacat ctaaacaatt agtaaaatct ttaagagaag
    63061 caggagcaaa agaaattcat cttagaataa cttctcctcc agtagcttac tcttgttact
    63121 atggaataga tactccaaat cgctctaaat taattgcatc aagtaacaac gtagaagaaa
    63181 tgagagaata tattggatgt gatagtttaa aattcctaga tatagaagga atgttagatg
    63241 caacagaaca taagtctact ttctgtaaag cttgttttga tggagaatac ccagttaaga
    63301 aaattgataa ggaggaacta ctttcatgtt aacatataaa gaatcaggtg tagatataga
    63361 tgaaggaaat agagcagtag accttataaa aggtaaaata aaaggaactt atgatggaaa
    63421 tgtagttgga gatttaggta acttcagtgg attatacagt ttaaaagatt ttgtaggtat
    63481 gaaagaacca gttttgcttg catctacaga tggtgttggg actaagttaa aaatagctca
    63541 gatgatggac aagcatgata ctgttggaat agatttagtt gcaatgtgcg tgaatgattt
    63601 aatttgtcaa ggagcaaaac cattattctt ccttgactat atagctcttg ggaaattagt
    63661 gcctgagcat atcgaaaaaa tagttggtgg aattgcagat ggttgtaaaa tgtctggttg
    63721 tgcactaatt ggtggagaaa ctgctgaaat gcctggaatg tatggagaaa atgactatga
    63781 tttagcagga ttttctgtag gaatagcaga caaagaaaag attgtttctg gaaacaatgt
    63841 taaatcagga gatgtattgg ttggtatatc ttcaagtgga gtacacagca atggattttc
    63901 ttttataaga aagatatttt tagaaactta caattataaa atggaacaat atgttgaaga
    63961 actaggaatg acagttggag aagcactttt aacaccaact aaaatatatg ttaaattagc
    64021 tttagatgta ttagctaaac atgacataaa agctatagct cacataactg gtggagggct
    64081 tatagaaaat ataacaagag ttatacctaa agggttagga ctagatatca ataaaaaatc
    64141 ttgggagaag cctcctatat ttaaaatgat agaaggtttt aatgctgttg atgagagaga
    64201 acttcataag agttttaaca tgggtattgg gctagtttta atagtagata aagaaaatgc
    64261 tgatgatgtt gtaaacttta taaataatag agaaaatgac aatgctgatt atgtagataa
    64321 aaaatatagt gaattattag aagataaagc ttatataatt ggtgaagtag tagatagtca
    64381 tgaaggtgta gaattatgct aaatatagga gttttaatat ctggtggggg aacaaattta
    64441 caagcagtta tagatggtac tgagtctgga gaaataaaag gtcaggttaa agtagttatc
    64501 tcaagtaagc aaggtgctta tgggctagag agagctaaaa atcataacat aaaagcaatt
    64561 tgtgaaactg atgaagacaa gataatagaa attcttaagg aaaataaaat agatttagta
    64621 gttttggctg gttatttaaa aataatcagc ccaaagctgg ttaatgagtt tagaaacaaa
    64681 atgataaaca tacatccatc attaattcct tcattttgtg gagctggatt ctatggagaa
    64741 aaagttcatc aaggtgttat agattacgga gctaaagtaa ctggtgctac agttcatttt
    64801 gttgatgaag gtgctgatac tggaccaatc ataatgcaag atgtagttaa ggtaaatcaa
    64861 gatgatgatg caaagacact tgcaaaaaga gtgttagaag ttgaacatag gattttaaaa
    64921 gaaagtataa gtcttttttg tgaaaataaa ataaaacttc aaggtaggag agtgtttata
    64981 aatgagtaag agagctttaa taagtgtaac tgacaagact ggtgtagtgg aatttgctaa
    65041 agagttaaat aaattagatt atgaaattat atctacagga aatacattta aaacattaaa
    65101 agaaaatggt gttaatgtaa tgcaagttga agatgttaca aattttccag aaatattaga
    65161 tggaagagta aaaacattaa atccatatat acatggtggt atactttata aaagagataa
    65221 agaatctcat gtagaaactg taaatgaaca caaaatacat tcaatagatt tagtagctgt
    65281 aaatctatat gattttgaag gaactttaaa agctggaaaa agtcatgatg aaataattga
    65341 aaatatagat attggtggac catctatgat acgttctgct gccaaaaact ataaggatgt
    65401 aatagtagta gtagatataa aagattatga ttctataata gaaaaattaa aaacagatac
    65461 aatgacatta gaagacagaa aaaaattatc atataaagct ttctcaacta caggaagata
    65521 tgatgcgtta atatctagtt attttgctgg agaagttgga gatacgtatc ctgacatatt
    65581 aaatttaact ttccaaaaag aacaaacttt aagatatgga gaaaatccac atcaaaatgg
    65641 tttcttatat tcacagtcaa atgcaaaaaa tccaatctta aattatgagc aattaggcgg
    65701 taaagaactt tcttttaaca acttaaatga tttacatgga tgtttagaag taatgagaga
    65761 atttaaagat agtgaagaag tagtttcagt agctataaaa catgctaatt catgtggggt
    65821 agggttagga aaagatgctt ttgaagcata tacaaaatgt tatgaagcgg ataaggtttc
    65881 aatatttgga ggaatagtag gaataacttc tactatagat aaagctacag cagagaaatt
    65941 aaatgaaata ttcttagaaa tagtagtagc atatgatttt gaaccagaag ctttagagat
    66001 acttaaacaa aagaaaaatc taagaatatt aaaattagct aaaatagaaa atagtttaca
    66061 accatatgaa atgaaatatt tagatggaaa attattgata caagatagaa acaatatatt
    66121 agcagaaaaa agtgaaaatg taacaaagga aaaaccaaca gatgcacaac ttaaggacat
    66181 ggaatttggt atgagagtag taaaaaatat gaaatcaaat gcaattgcaa tagtaaaaaa
    66241 tggacaaact ctagctttag gttgtggaca aacatcaaga atttgggcat taaaaaatgc
    66301 tcttgaaaat aacaaagata aagattttac aggagctgtt ttagcgtcag acgcattttt
    66361 cccatttgat gattgtgtaa cattagctca tgaatatgga atatcagcag tagttcaacc
    66421 aggtggctct ataaaagata aagattcaat agaagcttgt gacaagtatg atatggttat
    66481 ggtatttaca ggaataagac acttcaaaca ctaaggagga tattatgaag atattggttg
    66541 taggcggtgg cggaagagag catgctatat gctggaaact aagcaaagaa aaaaatgtag
    66601 aaaaaattta ttgtgcacca ggtaatgcag gaattgcaaa cgtagcagaa tgtgtaaata
    66661 taggtgatac taatatagaa gaacttttaa aatttgccaa agaaaatgaa ataggtctga
    66721 caatagtagg accagaagta ccactagtta tgggaatagt agatgagttt gaaaaagaag
    66781 gattaagagt atttggacct aataaaaaat gtgctcaact tgaaggaagt aaagcttttt
    66841 caaaagaatt tatgataaaa cataacatac ctactgcaaa atataaagaa tatacaaact
    66901 tagaagaagc tatatcagaa atagatagtt ttggatatcc agtagtaata aaagcggacg
    66961 gattagctgc tggaaaaggt gttgtaatac ctgaaaatag agaagatgct atagcaactt
    67021 taaaagaaat gatgtcagat aagaagtttg gagctgctgg agataaaata gtaattgaag
    67081 agtttttaaa aggaatagaa acttcaattc tagcatttgt agataatgat acaatagttc
    67141 ctatggcaag tgcaaaagac cataaaaaag taaacaacta tgagcaaggt ccaaacactg
    67201 ggggaatggg aactttttca cctagtgaaa tatatacaga agaattagca aataaagtaa
    67261 aagagacagt attagaaaaa actttagaag gcttcaaaaa agatggactt aattttaaag
    67321 gtattttatt tgttggtctt atgataactg aagatggaga aaaagtactt gaatataatg
    67381 taagatttgg agaccctgag acacaatctg ttttatttag attagaaaca gatttacatg
    67441 agataatgga agctatacta gataataagt taaaagatat agaaataaac tatagtgatg
    67501 atgaagctgt gtgtgttatg cttacatcag gagggtatcc agacagttat gaaaaaggga
    67561 aaattataac tggtcttgaa aacttggatg atgatatagt agtattccat agtggcacta
    67621 agatgtttga tggtaatttg gttaccaatg gaggaagagt aattggtata actgcaaagt
    67681 caactacagt taaggatgca gcagaaaaag tttatgaaaa cataaagaag ataaactttg
    67741 aaggaatgca ctatagaaca gacataggaa gataattttt ctaaaatgga ggataaggta
    67801 tgttgaatac tgaaaataaa gactcaatgg ttagaagagt attagttgaa aaaagagaag
    67861 gttttgacct tgaggctaaa gctttaaaaa aagacttagt agaaagttta cacatagata
    67921 atatagaaaa tttaagaata ttaaatagat atgatgtaga aggaatatca gaagaagttt
    67981 atgaaaatgc agcaaagaca atattctctg aaccaaattt agatgtagta tattatgaag
    68041 aaataccaaa attaaatgat gaaagagtat ttgcaataga atttcttcct ggtcaatacg
    68101 accaaagagg tgattgggca gctcaatgtg tacaaattgt aaatcaaggg ataagacctg
    68161 ctattaatac agcaaaagtt tatatattat caggcaaaat aactgatgaa gagttttcta
    68221 aaataaaaga ttactgtata aatccagtag atagtagaga agcatcttta gaaaaaccag
    68281 aaacgttaaa aatggaaact gaaataccaa caacagttga agtattagat ggatttatag
    68341 atttagatga aaatggatta aaaacatttg taagtgaaaa aggattagca atgactttag
    68401 gagacttaca acacgttcaa aaatacttta aagatacaga aaagagaaat ccaactataa
    68461 cagaaataaa agtattagat acttattggt ctgatcattg tagacatact acttttatga
    68521 ctgaaataga aaatgtaaag atagaagatg gtaagtttaa tgatatagta aaagaagcgt
    68581 atcaaatgta tcttaactca agagacaatg tatatgtgaa cagacataaa gatatatgtt
    68641 taatggatat agccactgtt gcagtaaaag aattaaagaa aaatgggaaa ttaaatgacc
    68701 tagatgaaag tgaagaaata aatgcatgta gtataaacgt agatgttgaa gttgatggta
    68761 agatggaaaa atatctagta atgtttaaaa atgaaactca caaccatcca actgaaatag
    68821 aaccatttgg tggagcagct acttgtttag gaggagctat aagagaccca ttatcaggaa
    68881 gaagttatgt ttatcaagca atgcgtgtta caggaagtgc tgacccaaga acgactttag
    68941 aagatacttt acctggaaaa cttatgcaaa gaaaaataac tacagaagct gctcatggat
    69001 atagttcata tggtaaccaa ataggtctta caactgggca agttgcagaa gtatatgatg
    69061 aaaactttgt tgcaaagaga atggaaatag gtgcagttat agcagcagca ccaaaagaga
    69121 atgtagtaag agaaagacct gaagcaggag atgtaatcgt tcttcttgga ggaaaaacag
    69181 gaagagatgg ctgtggagga gcaactggtt catctaaaga acatagtgaa gagtcaatct
    69241 taacttgtag tgcagaagtt caaaaaggag atgctcctaa cgaaagaaaa atacaaagat
    69301 tctttagaaa taaagaagtt gctcaaatga taaaaagatg taatgatttt ggagcaggtg
    69361 gagtttgtgt tgctatagga gagattgctg atagcttaga tataaactta gacttagttc
    69421 ctaaaaaata tgatggactt gatggaactg agcttgcaat ttcagagtca caagagcgta
    69481 tggcagtagc cataaaaaaa gaaaataaag ataaatttat acaattagca gtagaagaaa
    69541 acttagaagc tacacatgta gctacagtaa cagatacagg atatttaaga atgttctgga
    69601 atggaaaagc aattgtagat ataaatagag agtttttaga tacaaacgga gtaaaacaaa
    69661 ctactgatgt tcatgttact aaagtagatg aagaaaatac attctttagc tctaatgaaa
    69721 tagtaaaaga tgttaagtgt gcaagtatga aagacaagtt tacaaaagta ctatctgacc
    69781 ttaatgtttg ttctcaaaaa ggattagtag agatgtttga taatacaatt ggtggtaata
    69841 cagtccttat gccatttgga ggaaagtatc aagcgactcc aacacaaggt atggttgcta
    69901 agataccagt acttggagga gaaactaata cttctacaat aatgacttat ggatacaatc
    69961 caaaggtagg aaaatggagc ccattccatg gtgccttata tgcagtagta gaatctgtgt
    70021 gtaaattggt tgctattgga ggaaattata gtacaacaag acttacattc caagagtatt
    70081 ttgaaaaact tggtaacaat ccagaaaaat ggggtaaacc attctcagca cttttaggag
    70141 cattttatgc acaaagtaaa tttgagatac ctgctatagg tggaaaagac agtatgtctg
    70201 gtacatttaa agatattgaa gttccaccaa cattagtttc atttgctgtt gacacagtag
    70261 atgcgaagaa agtagtatct ccagaattta aaaaagcaga ctcaaaagta gtaatgttat
    70321 gtgtaaacaa ggcagaaaat gatgttgtag attttgagga attaaagaga aacttagata
    70381 aagtaagaga acttatacat ggaaataaag ttttatcaac atatgcatta ggatttgcag
    70441 gtgtaggtga agcaataagt aaaatggctt ttggtaataa aataggattt aagtttagtg
    70501 aagaggctga aaaagcattt acagatgata aattatttga ggctagttat ggaaatatag
    70561 tacttgaatt agcaaatgat gatttaagta tgttagaagg atacaattat gttgtgcttg
    70621 gaagtacagt taaagaagct agtatattta tcaaaggtga agaacttgca ttagatgaat
    70681 tatataaagc acattgtagt actttagagc ctatattccc aactaaaaca gaagaagtaa
    70741 aatcaaagat agaaactata agttatatat cacaaggaga agctaaaaaa tcatcattaa
    70801 gtatagctac tccaagagta tttataccag cattccctgg aacaaactgt gaatatgact
    70861 ctgcaagagc atttgaaaga gcaggagcta atgcaagtat aagagtattt aagaatttaa
    70921 cttataaaga catagaagat tctatagata caatagtaaa cgaaataaaa tcttctcaaa
    70981 taataatgtt accaggtgga tttagtgctg gtgatgagcc agatggttct ggtaaattta
    71041 tagctactgt atttagaaat cctagagtgc aagaagctat aaatgaattt ttaactcaaa
    71101 aagatggact aatgttaggt atctgtaatg gattccaagt attaattaaa ttagggttag
    71161 ttccatatgg agaaataaga gttccaagtg aaagtgctcc aacacttact tacaataata
    71221 ttggtcgcca tcaagctaag attgctagaa ctagaatttc atctaataaa tctccttggt
    71281 tagctcaaac taatgttgga gatattcata atatagcaat ttctcatgga gaaggtaagt
    71341 ttgttgccag tgaagatgtt atgagagaat taattgctaa tggacaagta gcaactcaat
    71401 atgttgactt taataatgaa gctacttatg atattgagtt taatccaaat ggttcattct
    71461 atgctgttga aggtataact agtgctgatg gtagagtgtt tggtaaaatg ggacattctg
    71521 aaagaattgg tgaggaagta tataagaata ttataggtga aaaagaccaa aagatatttg
    71581 aatctggagt taaatatttt agataaagct ttgatgttta attgatagct agaatatata
    71641 atttagatat ttagatttaa agagatgtaa taatatgatg ataatcattt aatattacat
    71701 ctctttttta tttatctaag aaataataag ttcaacctaa gttaaaatac aagctgtgtt
    71761 ataattaatt attaagtaaa attgaatatt tattggaggg caacataatt aatgaaaata
    71821 ttaataacag ggtctaatgg tcaattagga aaagaattgg ttaaccaatt agaagcaatc
    71881 aatcaaagta taaaccaacc aaaatatgtt atattagcta caacaagaag tgatttggac
    71941 atatccaatc agactaatgt agataatttt attttgcata ataaacctga tgtagttgta
    72001 aactgtgcag catatacaaa ggttgatgca tgtgaagata atatcgaaat tgcatacaaa
    72061 ataaatgctt taggagtgag aaatctagca atagcatcag aaaaagttaa tgctaagctt
    72121 attcacatat caactgacta tgtattcaat ggtttttcta aatatccata tagggaagac
    72181 aataaaactg aacccaattc tgtatatgga aaaagtaaac ttatgggaga aaaatttgtt
    72241 gaacaattct cacataagta ctttatattg agaacagcat ggttatatgg taatgggaat
    72301 aattttgtaa agaccatgat aaaactttct ttagaaaata cagaggttaa tgtggttaat
    72361 gaccagtttg gaactccaac aagtacagta gacttagcaa aaattataat aaaaatcatg
    72421 gaaacagaac attatggagt ttatcatgct acatgtgaag gtgagtgtag ttggtatgat
    72481 tttgccaaaa agatatttaa actaaaaaat atagatataa aagtaaatcc aattaagagt
    72541 aacgaattta aatgtaaggc tccaagacca ctatattctg tattggataa ttttatgttg
    72601 aagttaattg gtttaaattc ttttagaaag tgggaagaat ccattgaaga atatttagaa
    72661 agatatgata taaatgaaaa gtaaatgtat tattttgaga aaagaaaata agatattata
    72721 aaaaatataa atatttcttt tatttatccg ataatcataa tatgacaaaa acaaatttaa
    72781 ggatggaaaa agatgaaagg aattatttta gcaggagggt ctggtacaag actatatcct
    72841 ataacaaaat gtatatcaaa acaaacactt ccaatatacg ataaaccaat gatatattac
    72901 cctatgtcgg ttcttatgtt ggcagggata caagatatat taataatatc tacaccaaga
    72961 gatataaaag catttgaaga attatttgga gatggaagtt cactagggct taacataaca
    73021 tatgcaattc aggagaaagc aaatggaatt gcagaagcgt ttataatagg agaagaattt
    73081 ataggaaatg acagagtgtc tcttgtatta ggggataata tattttatgg atatggattt
    73141 agtgaaaggt tggagaatgc agttaaaaga aaaggagcaa ctatatttgg gtatcatgta
    73201 agtaatccaa attcgtttgg agttgtagaa tttgataagg attttaatgt attgtcaata
    73261 gaagaaaaac ctacagttcc taaatctaat tatgctatac caggattata tttttatgat
    73321 aatgatgttg tagagatagc aaagctagtt gaaccgtcag atagaggtga attagaaata
    73381 acctctataa acagtgaata tctaaaacga ggaaaactaa aagtggaact tctaggaaga
    73441 ggtatggctt ggcttgatac tggtacacat aaaggtctat tggatgcttc taattatgtt
    73501 gaagtggttc aaacaagaca gggactgtat atagcatgtt tagaagagat tgcatataca
    73561 aaaggatata taagtagaga acaattaatg aaactagcca agccactgat aaaaactgaa
    73621 tatggtcaat atctattaaa attggctagt gaagatatgt aatatggtag ataaaattta
    73681 ttataaattt tattaaggga gtttattata atgggaaaaa tcaatgttat aaagactaaa
    73741 atagaaggat tatatatagt cgaaccaaat gtattttatg atgaaagagg atattttatg
    73801 gaaagctata ataaaaagga ttttatagag cagggtcttg atatgtgttt tattcaagat
    73861 aatgagtcta gctcaagaaa aggcgtttta agaggaatgc attttcaggt taaatatcct
    73921 caaggaaaat tagtcagagt tacagaggga gaagttttcg atgttgcagt tgatttaagg
    73981 aaagattcac aaacatttgg gaaatgggaa ggtgtaatat tgtctgataa gaataaaaaa
    74041 caattttata ttccagaagg gtttgcacat ggatttttag ttttatcaga aaaagcagta
    74101 tttagttaca aatgtacaaa ttattattac ccagaatttg agaatggctt tatatggaat
    74161 gacaagcaaa tatctattga gtggccttta aaaggaattg aagaaatatt tttatcagaa
    74221 aaggacaaaa aacaaaaaag ttttaaagat atttgtacaa gacgttaata aaaatttatt
    74281 agaattaaca tttaggagaa aaaatatgaa aaaaatactg ataactggag gagcaggatt
    74341 tattgggagt aattttatac attatatgct taacaagtat gtagattatt ttattgttaa
    74401 cctagacttg ttgacttatg ctggtaattt agagacatta atagaaatac aagacaattc
    74461 caattataaa tttgttaaag gagatatttc aaatagggag caggtgtata agctatttga
    74521 agaagaaagg tttgatatag ttgttaactt tgccgctgaa tctcatgtag ataggtcagt
    74581 tgagaatcct gatttattta ttaaaacaaa cattatagga acacaagtac ttttagatgc
    74641 atctataaaa tatggagtta aaaggtatca tcaaatatct acagacgaag tgtatggtga
    74701 tttaccaatt gatagaaagg atttattctt tacagaacaa tcttctatca atccaagttc
    74761 tccatattca gcttctaaag catcagcaga tttattggta agttcttatt atagaacata
    74821 tggattgtta acaactatat ctaggtgttc caacaattat gggccatatc attttcctga
    74881 aaaattgatt cctcttatga ttttaaatgc tttggaaaac aaacaattgc cagtatatgg
    74941 gaatggggaa aatgtaagag attggttaca tgtatatgac cattgtactg ctatagactt
    75001 aataatacat aaaggaaata taggggagat ttacaatata ggaggtcata atgaaagaag
    75061 taatctagaa gtagtaaaaa tgatattaaa tcttttaggt aaatctgaag agttaatatc
    75121 ctatgtaaat gatagacctg gtcatgattt aagatatgct attgatgcaa ctaaaataga
    75181 gaatgagtta ggttggaagg caaaatatga ctttgatttg ggtataaaag aaactgttaa
    75241 gtggtatata gaaaatgaat cttggtggaa agctgtttta tcagtataac tttaaaattt
    75301 agaaaggggc gttttatatt gaatgagata agttcactaa ttaatatatt agcattgact
    75361 caattatctg gtagtaatac tacaaatcaa tgtaattgta catataataa ttcatctgga
    75421 tttgatatga ttatgatgac cttgttaaaa gcattatcac aaaataatca acatacttct
    75481 aatagctaca atttattgtc taattctgag aatttattta atgaacttga taatgtagta
    75541 aataatacca ctagtagatt tatagatgtt gatacaaaag ataaaaatgt aaagttgaga
    75601 attgaaaatg ctgtagatca agcatcaaaa aaatataacg tagattcaaa tcttataaaa
    75661 gcaataatta aagttgaatc agattttaat ccaaataccg tttcttctgc tggtgctaag
    75721 ggtcttatgc aacttatgcc agaaaactgc agagacttag gagttacaga cccttttaat
    75781 atagaacaga atatagatgc tggaactaga catattaagg aatacattga tatgtttgga
    75841 gggagcatag aaatgggatt gatggcatat aatggtggtc ctggaagaat gaaaagtaga
    75901 ggagtagaat caatctctga cctgtataaa atgccaaaag aaacacaaaa ttatattcct
    75961 aaggttatga aatattatag agggtaggct ttattatggg aataaatata gataagctaa
    76021 acataagtcc aagtataaat atggaggtta aaaaactaaa agagagtaat aaattttctg
    76081 acaaattaaa ctctttaaaa caatcttctt cattggaaga tataaaacga aatttggaaa
    76141 gtgtaaaaaa ggcaggagaa aggcttgtac ttactcaaaa ctatggcgat attagtatat
    76201 ataaaaatgc agtaaaagaa tacctaaaat ctgttgtaga taatatgtat tcacttgata
    76261 aaaatagtag cttttgggag catcagtatt ataagaatgt aaagattgtt gatagtaaac
    76321 ttgaggatat gactagtaag ttaattagtg aagaaaaaga aaacatagat attgtttcta
    76381 ctgttgacat gatacaaggc ttattgatag atatgtatag ataaggttat acaaggagaa
    76441 aaattttatg aatagcaaaa aagaggatat atatgaattg aattttgggc aattaataga
    76501 agaaaaatct ggtgttatag aagaaaatca aaatgttttt aatgcaaaag taaatgtagc
    76561 tgtttctata ggaaatacaa cagaaacaat aaaaaatata ttagatttaa aagaaggctc
    76621 tataataaaa ttaagtaaga atgtggatga aaaattagat atatatgcaa acgatagatt
    76681 gtttgcttat ggagaaagca ttattacaaa tgagaggtta tctgtaagat tatctaaaat
    76741 agaagatcca gatgtaaatt agcattttaa atagtatccc tttaggctat tctgtatgag
    76801 tttagctaag gggatatttt tataaaaaat ttaaaatatg tgtgaattat ataaatattt
    76861 ttattttatg agcgataact aaattataag tatgtaattt tggaggtgaa actatggata
    76921 ttaaaaatgt aagttcaaac attgtagtga ataattattt aaagaataca tctggtataa
    76981 aaaaagtgga agaggttaaa aaaattaata aaacagacaa gatagagtta tctaaatttg
    77041 caaaggaaat ggcaagtgtg tcaaatatga gtgatgagaa tattagaaat aaaaaagtcg
    77101 atgaaataaa aaatgcatta gaaaatggaa cttacaaaat aaaaccaaaa gaaattgcga
    77161 aaaaaatgat agaggatatg cgaggataaa gtcatgatta cagacttaaa agttataata
    77221 tatgaagaaa aaaatataat tagtaagatg atggaattat taaaagaaca atttgattat
    77281 atagttgaaa aagacttaga aaatttaaat atgttaaatc ctaaactaga agatattagc
    77341 agggagcttg catccataga aataaagaga agacaacttt ttggtgatga tatttcaatg
    77401 agtgaagttg ttgaaaatag taatgatgga tacttaaaag aaatatatac agacttgaaa
    77461 cacattcttg ctttagctaa aaatcaacaa gaatcaaatg attctctaat aaagaaagaa
    77521 cttattttta cgaaaaaaat gattaatttc ataaaaccag ctgataagaa aactacaact
    77581 tataattctt atggaaatat tagaaaataa atattaggag gagaaatatg gcaggattat
    77641 tttccatttt aaatacagct aattcaggaa tgaatgcgca ccaaaaagca atacagacaa
    77701 tttctcataa tataagtaat ctagatacag acggttattc taggcaaagg accgagtttg
    77761 ccacaaatag tccaatgtat atgcctagtt tgagtaattc aattgcaaga ggacaattag
    77821 ggactggagt acatgtaacg gatgtaacaa gagctagaaa tagtttttat gattaccaat
    77881 ttagggcaga agctcataag tatggtaaga cagtttcaaa atatgattat tacaatacta
    77941 tagaaactat actaaatgaa ccatcagact atggaatatc tgcaggtata gatgattttt
    78001 ttaaaggatg ggagtcatta tcaaaggata taaatagtga cagtaaaaaa agtctagtgg
    78061 tagaaaacgc atctaattta gcaactttaa taagtgaaag ttataagaag cttgataagt
    78121 taaaagaaga tatagattct aatatagaca ctgagataaa acaaataaat gacatgttaa
    78181 aaagtcttga agatttaaat aaatctattg atattatatc tggttctggc tctacaccaa
    78241 atgatttact tgatgaaaga gatagaattt tagataactt gagttttaaa ctagactttg
    78301 aaaatagtga tgttaaaaat atgcttgcag atggtaaatt agaacttaat gaattaaaaa
    78361 atccagatgg aacttggaaa acaggtatat cagggacctt acaaggcttg tttgaaatgc
    78421 atggtaagat tgatacatat aaaagcgatt taaaagatgt atctgatgga ttagctaaac
    78481 agataaatga tgtttataat acgtctgcag gaataacagt aacagatttt tttataactt
    78541 caaatgtaga tggtgaagat attataaaga taaatccagc tataaagagt aacccaaatg
    78601 aattaaagct tactgctgat gaagcttcaa aaattgctaa gttaaaggat gaaaaaatag
    78661 atattggggt ggctggtgga aaagtaagta caataagtga ccactataaa gcctttgcag
    78721 aatcaatcgg tttagattct caaaaagtaa atcaagatga agcaaatcaa cgtaaaatta
    78781 taaataatat tgataactca agaatgagtg tttcaggtgt atctttggat gaagaaatga
    78841 cagagcttat aaaaatacaa cgttcatatc aagctagtgc aaaagttatg tcaacagctg
    78901 tgcaactact ggatgttgta ataaatggaa taatttaata gaagggggaa tttataatga
    78961 gagtcagtac aggtatgatg tctagcagct atctaaactc actacaagat aatctacaaa
    79021 gattggataa ggttaataga caagttaata caacaaaaga aataaacaag ctatcagata
    79081 atccatataa agcaataaag atattaaact caaaaagtga aataaagact atggagactt
    79141 atgttgagaa ttgtaaagat acagggaatt ggttagaaac tactgatact gcattagatc
    79201 agcttggaaa attattatca gacataaaga aaactttggt ggcaagtggt gatggtgcat
    79261 actcagatga tgaattaaag actcttagta atgaaactaa tgaaaaaatg aaagaaattg
    79321 caaatgcttt aaatgcaaca catgaaggga agtacatatt ttcaggttca aatacggggg
    79381 taccacctgt tgaatgtata gataatgctg atggttcagt gagtttaaag tttaattcta
    79441 gtctaaatct taataaatta aatgactctt tgagtatgga tatagctcaa ggaataagtg
    79501 tagattataa cgttaaattg agtagccttg gatttgaccc aacaaaaact gacccatttg
    79561 aagagttaaa taacatatca aaaaaattag caaatccaga tgctgatgct ataaaagagc
    79621 ttacaacaac ttgtttgggc gatatggaac aactaataga aaatacagta aatgttaggt
    79681 ctatatatgg gacaaaagca aatacagtag aggctatgaa ggaaaaaaac gatgatagtt
    79741 tgatacagtt aaaggatatt ttgtcacaaa atgaagaaat agattatggt gaaaaacttg
    79801 ttcaattaaa ggcagcagag ttaacttatc aagcttcact acaaacagga ggaaagttat
    79861 ttaatgtatc tatattagat tacatataat aaaataacgc agatatgatg aaggttacat
    79921 taaaaaaagg tatattaggt tttgaaaatt taaaagaata tgagctttta gatatagaag
    79981 ataatgatta tttaaaagaa tttaattcta ctgaagaaga aggtatagga tttgttgttg
    80041 tatctccatt tgagattatc aatgaatatg aaatagtttt aaatcaggaa acaatagaaa
    80101 aactagaagt gaaatctcca aaggatgttg tattatttaa tataattact cttggtcaga
    80161 catttgaaga atcaactgtg aacatgaaag ctcctatagt gataaatata aaaactaatt
    80221 ttggaatgca aataatttta caggatgata aatattcaat aaggcatcca ttattgagag
    80281 gtgataatgg atgctagtaa tttcaagaaa aaaagatgaa gcagtactta taggagaaag
    80341 catagaagta aaaattgtag gagtagatgg aaacaatgta aaactagcta tatcagcgcc
    80401 taacaatata agtatattaa gaaaagaaat atacgaaaaa gtaaagagtg agaatataaa
    80461 agctacaaat aaaaatataa aaattttaaa gtcattaaaa taataagctt ggaggaagta
    80521 atatatgtat ggagaaaatc cttataattc ttataaacaa aatgctgttt ttatggcatc
    80581 aaaggaacaa ttgttactta tgcttgttga tggagcagta aaatatacca aaatagccaa
    80641 aacagctatc ttagataaaa atacacaaaa agcgcataga gagcttataa gagttcagga
    80701 tatatttaca gaactaatgg ttacattaga ccaaaatgca ggtcaatggg caaaggatat
    80761 gtacagagtt tatgattttg taagatatga actttcaaga gctaatatta gaaaagacat
    80821 tcaaataata gataatgtgt tgcctgtaat agaacaaatt aaagatactt ggcatgaagc
    80881 tgataaaaaa agtaaagaag aaagaagtag gtacaaataa tggaattaaa tttagctgaa
    80941 ttagataagt tatcaaaaga ggaattacta ttgatgcttg tagatggaac agtaaaatat
    81001 accaatatat ccaaagaagc tcttttaaat aacgactatt tgaaagcaca taatgaactt
    81061 gtaagagttc aaaatatatt tacagaacta atgactacat tagaccaaga tgcaggtcaa
    81121 tgggcaaagg atatgtataa agtttatgaa tttataaaat ctgaattagc aatagctgat
    81181 gaaaataaag atattaaaat aattgatgat atattgccaa tagtaaaaca aattagagat
    81241 acatggcatg aagtttacaa tcaactaaaa atataattgg atggagggat aaaggtatgt
    81301 caagtataag tcctataaga gttacaggtc tttcaggaaa ttttgatatg gaaggcataa
    81361 tcgaagctag tatgattaga gacaaggaaa aagttgataa agcaaaacaa gaacaacaaa
    81421 tcgttaaatg gaagcaagaa atatatagaa atgttataca agaatcaaaa gatctttatg
    81481 ataaatatct aagcgtaaat tctcctaata gtatagtaag tgaaaaagca tactcttcta
    81541 caagaataac cagttctgat gaaagtatta tagtagcaaa aggctcagct ggtgcagaaa
    81601 aaataaatta tcaatttgca gtttctcaaa tggctgaacc agcaaaattt actattaaat
    81661 taaattcaag tgaacctatt gttcaacagt ttcctccaaa tgctagtgga gcgagttctt
    81721 taactatagg agatgtaaat ataccaatat ctgaacaaga tactacaagt actattgtaa
    81781 gtaagataaa ctccctttgc gcagataatg atataaaggc ttcttatagt gagatgacag
    81841 gtgaattgat tatttcaaga aaacagactg gttcgtcatc agacattaat ttaagagtaa
    81901 ttggaaatga caatttagct cagcaaattg ctaatgataa tggtatcaca tttataaatg
    81961 atgctggtgg aaacaaagtg gcaaatgtat atggaaagaa tctagaagct gatgtaactg
    82021 atgaacatgg aagagtaact catataagta aagaacaaaa ttcatttaat atagataata
    82081 ttgactataa tgtaaattca aaaggaactg caaagttgac ttctgtcact gatactgaag
    82141 aagctgttaa aaatatgcaa gcatttgtgg atgattataa taaactgatg gacaaggtct
    82201 atggtttagt tactactaaa aaaccaaaag attatccgcc tcttacagat gcccaaaaag
    82261 aagatatgac aactgaagaa atagaaaaat gggaaaagaa agctaaagaa ggtatactta
    82321 gaaatgatga tgagttaaga ggttttgttg aagatattca atctgcattt tttggagatg
    82381 gaaaaaatat tattgcatta agaaaactag gtatcaatga aagtgaaaat tacaataaaa
    82441 aaggtcaaat atcatttaat gcagatactt tttcaaaggc tcttatagat gatagtgata
    82501 aggtatacaa aacactagca ggttattctt cgaattatga tgataaggga atgtttgaaa
    82561 agctaaaaga tattgtatat gaatattctg gaagttcaac ttctaaactt cctaaaaaag
    82621 caggtataga aaaaactgct tctgctagtg aaaatgtata ttcaaaacaa attgcagagc
    82681 aagaaagaaa tataagcagg ttagttgaaa aaatgaatga taaagagaaa agactttatg
    82741 ctaaatattc agccttagaa tctttgttga atcagtattc ttcccaaatg aattatttct
    82801 cacaagcaca gggtaattaa ttatggataa attaaaagaa aaattaaatt tatataaaga
    82861 tatatcttta caaattataa atttgattga aaaggaagaa tatataaaga taagtattaa
    82921 gttaggtgaa agacaagaga ttataaatag tgtaagtgaa attgatagaa atgattttat
    82981 acagctttat aatagaatgg aattaattga aattgatagc aggataagag atattttaca
    83041 aggacaatta ttagaagtaa aaaaagaatt acatgagtat aagcttacta aacaagtgaa
    83101 cacaatgtat tataacttaa atagagaaaa agtaaatatt tttaataaaa aagtataaag
    83161 ttatgtacca tcttgtccga ttatataaat gaagaattaa taaaaaagat taaaagtaga
    83221 aatgacaagg atgtcaacta tactaaggag ggtaaaataa tgagagttaa tacaaatgta
    83281 agtgctttga tagcaaataa ccaaatggga agaaacgtaa atgcacaaag taagtctatg
    83341 gagaagctat cttctggtgt tagaattaag agagctgctg acgatgctgc tgggcttgct
    83401 atatctgaga aaatgagagc tcaaataaaa ggtttagacc aagcaggaag aaacgttcaa
    83461 gatggtatat ctgttgtaca aacagcagaa ggtgcattag aagaaactgg taacatacta
    83521 caaagaatga gaactttatc agtacaatct tcaaatgaaa ctaatactgc tgaagaaaga
    83581 caaaagatag ctgatgagtt actacaatta aaagatgaag ttgaaagaat atcaagctca
    83641 attgagttta atggtaagaa attacttgat ggaagttcta cagaaataag attacaagtt
    83701 ggagcaaact ttggaacaaa tgttgcagga acatctaaca acaacaatga aataaaagtt
    83761 gctctagtaa atacttcaag tataatgagt aaagcaggta taacaagttc tacaatagca
    83821 agcttaaatg cagatggaac ttctggaaca aatgcagcta aacaaatggt atctagctta
    83881 gatgtagctc ttaaagaatt aaatacatca agagcaaaat taggggcaca acaaaataga
    83941 ctagaatcaa ctcaaaacaa cttaaataat actatagaaa atgttacagc agctgaatca
    84001 agaataagag atacagatgt tgcttcagaa atggttaact tatctaaaat gaatatatta
    84061 gttcaagcat cacagtcaat gcttgctcaa gctaatcaac aaccacaagg agttttacaa
    84121 ttattaggat aaaataattt tgtatattgg atatagaaga aagggtaagg ctttgcttta
    84181 ctcctttctt tttttacaaa tatacttttg aaaggaaaat ttatatgtta cttagtatag
    84241 ttatgattgt taaaaatgaa gaaaaaatac ttgaaaaaac tttaaaatca ttaaccacat
    84301 taagaaattc tatagaaagt gaacttataa tagtcgatac aggttctaca gatgatacta
    84361 taaaaatatc taagaagtac acggaaaaag tgtattttca taattggaat aatgattttt
    84421 ctagtatgag aaatatatca atatcctatg ctaaaggtga atggttactt atattagatg
    84481 cagatgaaat actaattgat tcttcaacta ttgtaaaatt ttttaaagat ggtcttgata
    84541 aaaaatttaa ttctgcttca atacaattaa aaaatcttta ctcatatgat aaaaaattat
    84601 atggatattg ttcggtgctt agacttttta aaaacgttga ttttagatac catggtaaag
    84661 tacatgaaca accaatatac aaaaacccta tatttaataa tgttgctgat tttgaacatt
    84721 atggatattt gtttgaagat gaagaaataa gattaaataa ggtgaaaaga aatgaaaaac
    84781 tattatttca agagttaaaa gagaatgata attctccata tacaaactat caattaggga
    84841 aaaattttat tatattagga aaatatcagg aggctttata ctatcttgag aaatcttatg
    84901 aattatattc taaactagaa ttggcacctg gttacttgat aacaagcctt gtaaaaacat
    84961 atttatattt aggaaaacat aagaagtgtg aaagattgtg cttaaagtat attaagaaag
    85021 acatgaataa tattgatata tattattata ttgcacaagc acaagtagat ttaggaaagt
    85081 atgaaaatag tatagattcg tataagagat atatttactt gttagataat tatgaaataa
    85141 gtacacaagc aaatagttta ttttcagaca ctgatactgt agggcttaga gatgaggcta
    85201 ttattacact gataaagatt tattataagc ttgaaaaata tgagtttgta atttcagagt
    85261 ataataacat tgaagatgtg gaaaagaaaa agaatgtgta ctttagctta tttatgtcac
    85321 tatataaatt gaatagattt gaagatataa aaaattatta taaagaagtt tcaaattcta
    85381 aaattgaaaa aaatagtttt tatagaaata tagaagaagt tataaataat attaaagagg
    85441 atgaaaaaga gtatgtatat aaaatccttt gtgacattga aggtaattat ggcattttaa
    85501 atcaaataag gataaataaa tgtgtatcta tagataaatg taaagaaata ctcaatgaag
    85561 aaaaggaaat aatttatgca tatattataa aaatagcttt tgaagagaag attgatttac
    85621 tagatatttt ttatgaaatg gaatatgtat ggatagagaa atatctaaaa tatcttttag
    85681 cattagaaag aagttttaat ttagtactat ataagtacat aattagtcgt ccaaatacat
    85741 cagatatgga aaagataaga gtatataaag ctatatctaa agtagtttta gaaagtataa
    85801 gcttaagtga tgaaaaatat aaggagattt tttatcttta tataatgtat tcttataagt
    85861 acataagata catatatagc aatttaagtg attttgaatt aataaaattt gtttgtaatg
    85921 atttagataa gttcactata gaatttaagc atctacttga tattaaaaat aaagtaaata
    85981 atgctaatga aaaattagag tatatacata gtatgaggaa attactaaat gagtatcctt
    86041 tttataataa aataataaaa atacttatag gtgaattaga agaaaatata aaagaaagtg
    86101 aagaatttaa ggatttaaaa gatagtttta ttattagtat agaaaatatg attgaaactg
    86161 gcaaaataaa tgatgcgaaa ttattggtag atgactattc aaaaacattt gtggatgata
    86221 taaagatttt aaatataaag ggaattttat acatgtttga aaataagctt gataaagctg
    86281 attttatgtt aaaaaaggca ttatcattag atgtagaaaa tgaagatact atatataata
    86341 tagaatactt aaaaagttta agataagtca aaaataggag aattgaagaa tgaatatacc
    86401 aatagtggtt aatgaaaaat ctaaagacat aataaaagat tggcaatata catgtacaaa
    86461 tgaagatata aaatttacaa gcataataat attgacatat aatcaaatag aatatactaa
    86521 attatgtatt gaaagtatta ggaaatttac cccaaagaat aaatatgaaa taataataat
    86581 tgataataac tcttctgatg gaacagttga gtggttaaag ggacaatctg atataaaatc
    86641 tgtgtataat aacaaaaact taggatttcc aaaaggatgc aatcaaggta ttgagttagc
    86701 tactggtgaa aacatattgt tgttaaataa tgatgtaata gttactccaa attggctcta
    86761 taatttgaat aaagctttat ggtctcaaga taatataggt gctgtaggac ctattacgaa
    86821 tgcatgttca tattatcagc aaattgatgt tgattataag tcaattgagg acatgtttat
    86881 atttgcacac aaaataaata aatctaatat taacctatgg aaatatagat tgaagttagt
    86941 tggattttgc atgttaataa aaaaaactgt tctggataat gtaggggttt tagatgaatt
    87001 attcacacct ggaaattttg aagatgatga tttatctttt aggatgatag agtcaggata
    87061 taagttatta ttatgccagg atacttttat acaccatttt ggaagtgtgt cttttaaaga
    87121 aaatcgtaat aaatatacag atataatgta tattaataga agaaaatttg aaactaagtg
    87181 gggatttaat ccagaatata gtggaatgat aagaacagat ttgatttcta aaatagatga
    87241 tagtttggag gcaaatatta atgttttaga aataggatgc ggtatagggg ctactctttt
    87301 agaaataaaa aatatatata aaaatgcatc aatatatgga atagaaatta gtgagtcagc
    87361 aggtaaactt gcaaagtata gtgcagatgt acttatagca aatatagaaa aagtaaaatt
    87421 ggattataaa aaagattttt ttgattatat aatattggga gatgtacttg aacatttgat
    87481 tgacccatgg aagatagtca atgaattgaa acaatatcta aagaaggatg gttatctggt
    87541 atctagtata ccaaatatta tgcatgtttc tgttttaaga aatcttgtgt taggtaattt
    87601 tacatatgaa gattcaggaa ttttggacaa gactcattta aggtttttta cattaaatga
    87661 gataacaaaa atgtttaatg atagtaatta cataattaat agtattacag ctactagtat
    87721 gccactttcg gaaattgata atagcttcat tgatacaata tgtaaggaaa gctgtaaaga
    87781 aatgagacaa cagtacgaat cttatcaata tatagtaaaa gcctctaaaa tcctagatac
    87841 taaaagatat tctagtatta gtatgattaa tttaaaatac gctttaatga gaattgataa
    87901 tgaattagat gtagatagta atttaagtta tatatttgat aattataata taactgaacc
    87961 atgttttgtt gatgatattg aatatctaat tgatacattt gtgataaata aagcaactct
    88021 attaaataga ttaggagttg aggcatttaa cagaggtcta ggagatttta gtataaacat
    88081 gtttatgaca gtattagaga tggatagaga tgatattgac tcaatatata atataacaag
    88141 tgtattatgt gaattagaag aatttgatac tgcttataat ttaattaaaa atagtagcga
    88201 aaaagtacag agtgaaagtg atatacaaga gatactttgt tttattgagg ggaagaaata
    88261 tgaataatga taagatatgt tttataacct gtgtaaatga tgaaaggctt tatgaagaat
    88321 gtcttgtata tataaatagc ctagacattc ctaaaaactt tagtatagaa acagtagctt
    88381 taagaggagc cgattctatt gcctctgctt ataataaagc tatgagagaa acagattcta
    88441 agtataaggt atacttacat caagatactt ttataattaa taaaaatttc atacatgata
    88501 ttttagagat atttaaaaaa aatacagatg tcggaatgat tggtgtagct ggagtaaaag
    88561 atatgcctgt gaatggaatt tggtgggatt ctgataaaaa agcagggaaa gtatatgata
    88621 gtcatacagg taaaatggat atttatgaat ttagtaactt tgaagaatgt tttaatgaag
    88681 ttatggctat agatggatta attatgatta ctcaatatga tgtattttgg agagaggata
    88741 agtttgatgg gtggcatttt tatgatttgt cacagagtat ggagtttaga caaaataaat
    88801 acaaagttat tgtaccaaaa caaaataagc cttggtgtat gcatgattct ggagtagtaa
    88861 atataaataa tggatatgat gaatatagga taaaatttgt aaaagagtac ataaatcttg
    88921 atttatatag agaaaatttt tatgaatttg ataagaagaa agcaagtacc atacttaaga
    88981 gtaataagca tgtaatggat tgtatgaaag aggagcttac agaacaatta agattaaaga
    89041 attacaagga tgttttaggt attgtttcta aaattgctca tttttctgtt tttaatcata
    89101 ctggtttgta ttcttgtgaa gaatgtgaga gagcattgct agaatgtgca aataatttac
    89161 cagaaataga aaatacgaaa ttatttacaa aaaaagattt aaaaagaaga aaagtattac
    89221 atgttttaag tgaaggatat tcaacaggag gtcatacaag actagttaaa aattggattg
    89281 aaattgacaa agactctgtt cattcagttg taactacatg gcaatcagat acactgccag
    89341 actggttatt aaaagcaata aatgatagtg gtggcgaagt tatatctttg gatttaattt
    89401 cagatgatta tttaaaaaga gctgtagctt tgaggaaaat agcttatgac tgggctgata
    89461 tagttatttt atatgttcat atgtgggata caatacctgt tgttgctttt ggagttgatg
    89521 ggaaaatacc tattttacat gttaatcact atgatagtac tttttggtta gggacaagta
    89581 ttgcagatat tgtcattgat tttagcgaaa ctagtcaatt aattacatta gataaaagag
    89641 gcatagataa atctgaaata ttaccaatgc cattaaaaga aaaagaagta ttttcaaaat
    89701 ctgaagttag aaaacgatat aatttagagg atgatgtgac aataatattg actattgcta
    89761 gtgaaagaaa atacaatagt gtaaatgata ttaattactt taaaattgct aaaacattga
    89821 ttgaaaatac agataataca attattattg ttgtaggagt tgatacagag aatctctatt
    89881 ggaaaacttt aagtaatgtt acagataata gagttatatg ttttggaaca acagcaaata
    89941 tagatgattt ttataagatt tcggatatat acctagagtc tttcatggta ggaagtcata
    90001 cttcaaaatt agatgcaatt ttatatgatt taattcctgt taagtttaaa aatgaagttt
    90061 caccaatttt tacaaatatg tggaatttta tagataagtt tgattataag aatataaaag
    90121 aggttataga catgataaga aggtacaata ctagtgaatt tgacagggat aagataaaag
    90181 caaaacaatc ggaaataaaa cattttgtta tagataaaca ttgtattgaa ataaaaaact
    90241 atatagacaa aatatataat gaagttaatc atcataaagt taagtatgat ttatataaaa
    90301 atgatgaaat cgaggactat cagctttttg tagcattata tggctatatg gtaaaaaata
    90361 aaaacatgtc ataggtggta aaatgaacaa tattcatatc aataaatttg aaaaaagctg
    90421 tgaaaaaaac tgggattatt ttgtagaaaa taaatctata aatggtacat ttctgcaaac
    90481 tagaaatttc cttaattatc atgaggaggg aaaattcact gaccactcat taataatatg
    90541 taaaggtgaa gaaatactag cagtcgtacc tgcatgtgaa acttatgaag atggtgaaag
    90601 aatatttttt tctcatttag ggagtacttt tggtggcatt ataataagaa gtggttttaa
    90661 tgatattagg catgtaaatg ccattacaaa taagttagaa atatatttac aggaacaaaa
    90721 tttcgataaa gtatatttaa aaaatacatc aagaatattt tgtaaagagg atacttcatt
    90781 actagaatat tttttattta agaataatta catgttttat gatgaattaa gtatgttttt
    90841 agaattaagt aagtataaag aagatatatt agccaatttt aaatacagta aaagaagaga
    90901 ttttaaatat tctttgaaga ataatcttac ctttaaaaga ctgtatagtg atgaagatat
    90961 agtttgtttt tttaatttac tttctaattg tttagatagt aaaggagcta aagtaataca
    91021 ttcagtagaa gaacttttgg agtttaaaaa taatagatta aaagatattg tagaatttta
    91081 tgcagtatat attgatgaca ttatgatttg tgggagcatg gtatttaact ttcaagaaag
    91141 agtttatcat actcaatatc tagcatcaaa ttcgacatat aataaatatt atccaatgat
    91201 atttttatac tataatctaa taaaagatgc tttagagaga aattttaagt atctttcttt
    91261 tggaattagc actctaaata gaggcaaata cttagatgag ggtttagcta catttaaaga
    91321 aggttttggt tcaagtgcaa tttgtaacag aacatactat aaaaatataa ataagtgaga
    91381 atcatgtttt atagagtttt tagagtatag taaatatcta gtactataat attacataaa
    91441 aataattagt ttataaagaa ggtttgtata tgaaaagatt aatgatttta ggctcaatta
    91501 agtattttga gaatattgtt atgtcagcta aaagagaggg aatatacaca attgtttgtg
    91561 ataatagaat tgatactcct gcgaaaaaga tttgtgatga agcaatagat gttgatgtat
    91621 ttgattttga taaaataaag aatatagcta tagataaaaa gatagatgga attttaactg
    91681 gatttacaga ttctttaatg aagccatatg tttatattgc aaatgaacta aatttgccat
    91741 gtgtgatttc ttgcaatcaa cttgaatctg taactaataa ggctgttatg aaaaattatt
    91801 ttaaaaataa tggaatacct acaactgatt attgtattat tgaaagtttg aaagatttag
    91861 aaaaaataca aggattaaat tttccacttg taataaaacc tgttgatggt tatggttcaa
    91921 gaggtgtata ttttgtatca aacatagaag aattaataga aaaatttgaa tattctaagg
    91981 aagcctcgca agatggacaa gtaataattg aggaatttta tgaaagtgag gaaatacaag
    92041 gactagcttg ggttcataat ggagagtcac atgtatttta tattggagat agagagcttg
    92101 taaatataca tacaggaaga gctggaaaac cagatagatt aatttatcca tcaaagtatt
    92161 gttttcaata tgaagaagaa ataaaatcca tatatcaaaa tataacgcag gcttttggca
    92221 taaaaaatgg acctctttat gtacaaatgt tagtgggagc atatggaata aaggtatctg
    92281 aagtaatgcc aagattacct ggaggttgtg actatatggc tatttctcaa ataacagatt
    92341 tagacatagg aagtttattt gcaaacttta gtgtgaataa tgaagtaaat tttgatgaaa
    92401 taaagaaata taatatgcat ctatcaaaat gtgtatatgc attaccaata tacataagac
    92461 ctggcacaat aaaaaagata aataatatag ataaaataga aaacttagat tatgtcactc
    92521 aatttctatt actagtaaaa gaaggagata caataggatt atatgaagat gagagacagg
    92581 attgtggaag agtttatgga gtagcaaata atgtatttga tgcaaatata aagaagaaat
    92641 gtattcatga aatgatagaa atacttgatg aaaataatga aaatatgata gaaaattttt
    92701 aaataaggga gtatgataga tatgaagcta ataactcatg aacaaattat gtcattaaac
    92761 atttctcaga aacaaggata cgagtggata aaagaatcct ttttaacaaa aaaagattgt
    92821 caattaccac caaaaataag catggtagaa gaagggcatg tattttacaa tgtaatgccg
    92881 tccctaaatc ataatcataa gattggtgga ataaaaatgg ttacaagatt gccagggaga
    92941 aatccaagtt taaattcaga gatatttcta tataatatgg aagatgctaa attaaaggct
    93001 attttagatg gcaattatat tacagctttt aggactgctg ctgttgctgc cttggctatc
    93061 gatacatttg ctaaaaaaga ttttaatact attgctatta tgggtttagg tgtaactgct
    93121 tctgctataa tgaatatgct ttcatatact cttaaagata aaaatataaa tataaagtta
    93181 cttaaatata aagaccaagc agagtcgttt gtaaaaaagt atgaaaaaaa tgaacaattt
    93241 agttttgaaa tatgtgacac acatgaagaa cttattgtag attcagatgt agttatttct
    93301 actgtgacat atacagatga atatttatca gaatttaaat ggtttaaaaa aggtgcttta
    93361 ttattacctg tacatgttat gggatttgga aatgttgatt atctatttga taaagtatat
    93421 ttggatgatt ataatcattt gaaacatcta gaaaatatag atagatataa atattcttgt
    93481 gaattttatg atgttttaag taaacaagct aaaggaagag aaaatgatga tgagagaata
    93541 atatcttaca atataggttt gtcaatacat gatatattat ttgcaaataa aatttacgaa
    93601 ttgtgtgaaa cactaagctg tgaagaaata gaattaaaag aaccaaaaga aaaatattgg
    93661 atatagttta tttcattcat atttacaata gtttattgta aatatgaata tgaagtaata
    93721 tattgttaga aaattagcat gaataggata gataaattta tggataaata taatttaatt
    93781 aggttcgtat ctattggaaa cgaatatata ggaaatttag ttgcattaga atcaaataaa
    93841 gcaatacctt ttgatataaa gagagtttat tacatatatg atgtaccaaa caatgtaaag
    93901 agaggatttc atgcacacaa aaacttggaa caagtactag tttgcctaaa tggaagtgtg
    93961 aagataaaat gttatgatgg tgtaaaagag actatattta atttacataa aaatgatgtt
    94021 ggattatata taggaacggg agtgtggcat gagatgtttg attttgatga aaaaactgtt
    94081 ttaatttcta tagcatctga acattataat gaagaagatt atattagaga ctataaagaa
    94141 tttttaaagt atttaaataa tatgtagtta ttatacagtt agtgatgtat tttcttaatt
    94201 aaataaattt aagatagcca catagagaat agatagatta ggatgtgaat ttattgttgg
    94261 ttaaatttaa taatttatta aaacagttcc aaacccataa agaagaatat gaacagaaga
    94321 ttaaggatgt aatggaaagt ggatattata tccttggaaa tgaagtaaag cattttgaag
    94381 aagaatttgc taactatgta ggtagcaaat attgtgttgg tttagccagt ggtttagatg
    94441 cactgataat tgcttttaga gcattaggaa taagcgaagg tgatgaagta atcgtgcaat
    94501 caaatacata tattgctagt gttatgggaa tcagtataaa tggagcaaca ccagtatttg
    94561 tagaaccaaa tgaatattat aatatagatt catctaagat agaagaaaag ataaatagca
    94621 aaacaaaagc tatattagta gtgcatttat atggtcaagc aagtaatatg aaagatatac
    94681 aagaattatg tgataagtat aacttgtatt taattgaaga ttgtgctcaa tctcatggag
    94741 ctaaatatga tggaaaaatg acaggaacat ttggtgatat aggttgtttt agtttttatc
    94801 caagtaaaaa tttgggtgca tttggagatg caggagctat agtaactagt aatgaggaaa
    94861 tagctgataa agtgaaaatt cttagaaact atggctcaga aaagcgatat tataataaat
    94921 ttgtcggata taattcaaga ttggacgaaa ttcaggcagg tttattaaga gtaaaactta
    94981 aatatctaaa tgaaattgaa attgaaaaat caaaaatcgt agatagatat cttaatgaaa
    95041 ttaataatcc caaagtcata cttccaaaga tagaaaataa ttgtactcat gtatggcatt
    95101 tatttgtttt acaagttgaa aaacgagata catttatgga atacttaaag aaaaatggta
    95161 tagagtctat aatacattat ccgataccac cacatctatc tgaagcatat gcttatttgg
    95221 gatataaaaa ggggagtttt aatatagctg agaactattc agataaagtt gttagtatac
    95281 ctttatttat cggtatggaa gatgaagaag ttagttatat aattaaaatt ataaatgaat
    95341 attaaactac tcataataat atcaaaaata aattttttat atatcattta tttaagtttc
    95401 gttaattgac atttttaa
//
LOCUS       DBGJFF010000004        61083 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.004, whole genome shotgun sequence.
ACCESSION   DBGJFF010000004 DBGJFF010000000
VERSION     DBGJFF010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 61083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61083
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.004"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            81..2009
                     /locus_tag="ACOIAF_000355"
     CDS             81..2009
                     /locus_tag="ACOIAF_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HGZ2667390.1"
                     /translation="MHKRLLTLFKLLNESDDKITCKTLSNHLKVSERTIRNDITSING
                     TLEKNGAIIKIKKGEGYYIDILNLALYQQYLALISDDIMDSSEIPDSPIERNQYILKY
                     ILYNNTYIKLEDLANSLYVSKFTILNDIKRIKPILSKYNLILVSKPYYGVKVEGKEID
                     IRRCISNNMINRNFENYIIGITDREIELFNNVDLIELQRVVLSEINKFNINFLDFNLK
                     NFIIHLAITISRILDGYCLDNVLDVVLTDFQSNTTVENIFNYIESKYTIIISKADRVY
                     LYNHFITKSSLLDNVSNRVDTKIIEYVEEILEVINNQYTFDLRNDSVLFDDLVLHFKS
                     ILNSKSYNLNKVNPLINTIKSNYPLAFEITLNAIEKVFKNSIYSLTEDEIGYVSLHIG
                     AGIERFFQNNIKCKNVVLVCGSGYGSSRLLEVQLNKVFHDKINILQCLSFNQFLASEL
                     SDVDIIISTIPLNHDSIPIVLVDLKLLKKDIENISKSITNNSHIYSNLLDNFFDKNLF
                     IVNPKIKDKDELIKLMCNKLTQSEIVFPSFAESVFYRESLSSTNIDDFLAIPHPMELS
                     SIRTKICISILNEPIYWSEDSTVKLIMMLAINKDDYIKINSIYDILLKIIHDNDIRDS
                     ISNCNDFDNFLSIIKSIV"
     gene            complement(2269..3030)
                     /locus_tag="ACOIAF_000356"
     CDS             complement(2269..3030)
                     /locus_tag="ACOIAF_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HGZ2667391.1"
                     /translation="MEYTVQKLSKLAGISTRTLRYYDEIGLLKPLKINSSGYRIYGQN
                     EVNKLQQILFYRELGINLENIKNIINSPNFDSLSALKEHHNKLLAKRKQIDLLIENVT
                     KTIALKEGKYTMTDIEKFEGFKEKMIDENEKNYGTEIREKYGKDIIDTSNKKLKNMSK
                     QDYENWQNLNIEIISKLKKAFKTGDASSELAQEVAKLHHKWLSYTWNTYSKEAHSSLA
                     QMYVYDERFTSYYDREQPGLAKFLRDAIVIYTRDK"
     gene            3249..3725
                     /locus_tag="ACOIAF_000357"
     CDS             3249..3725
                     /locus_tag="ACOIAF_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HGZ2667392.1"
                     /translation="MNLKRSNEKREEYRRMYSDKESFLSLIQNFTSVSIAKELTLKNI
                     ELETSFICEYKGKEVDIIYKVFSKSGKVSHYIVLEFQTEMDTEIVPRLKSYREQIWKS
                     FIMKKSLEEIEDKNFKLPKVIPVVLYSGPERLSRKKGILDIVESISEFSNNSSMKN"
     gene            3823..3975
                     /locus_tag="ACOIAF_000358"
     CDS             3823..3975
                     /locus_tag="ACOIAF_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate translocator"
                     /protein_id="HGZ2667393.1"
                     /translation="MRNNKSKVLLGILCLATGMAVILSMLLPGWIWSALTALILIGCG
                     ALLFFC"
     gene            complement(4135..4323)
                     /gene="rpmB"
                     /locus_tag="ACOIAF_000359"
     CDS             complement(4135..4323)
                     /gene="rpmB"
                     /locus_tag="ACOIAF_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HGZ2667394.1"
                     /translation="MAKVCSVCGKGKVSGNQVSHSNKHNKRTWSANLRSVRAIIDGAP
                     KRVKVCTRCLRSGKIERA"
     gene            4684..5034
                     /locus_tag="ACOIAF_000360"
     CDS             4684..5034
                     /locus_tag="ACOIAF_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HGZ2667395.1"
                     /translation="MSAKVTNQYGNIEIDNQVIAQVVYRAAMESYGLVGLAYKSKGIV
                     ELLKGENATKGVRVEEVEEDAIAIELYVIIQYGTNISTVANNIIDRVKYVVEKMTGVR
                     VTRIDINVQGIRVK"
     gene            5053..6696
                     /locus_tag="ACOIAF_000361"
     CDS             5053..6696
                     /locus_tag="ACOIAF_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HGZ2667396.1"
                     /translation="MIQYIDGKRLREMFISGANNLQNNKELVDKLNVFPVPDGDTGTN
                     MSLTISYALKELAKVENDNISDIGKALSKGSLMGARGNSGVILSQIIRGIAKSIEGKS
                     KLSTEDLAKAFKNGSDTAYKAVIKPIEGTILTVVRESGEFAIKTAKKEKDVVKFLSML
                     VKESNSSLERTPDLLKNLKEAGVVDSGGKGLVLIYEGMLASIKGDNIEIKNVDLDTNI
                     STSMDFAKSTTSTDDIKYCYCTEFILESSKVEDTKIRDIMMAYGDSLAVVGDDGVIKV
                     HVHTNDPGNVLQEALKYGQLLTIKIENMKLQHENTLLDVEEKKENDSEPLEEEKEFGF
                     IATSMGEGLANIFKDFGVDHIIEGGQTMNPSTEDFMNAIKHINAKNIFIFPNNSNIIM
                     AANQAKELSDKNIIVIPTKNTPQGFAALVTFNGELSEDENKEAMMNALNSVKSGQVTF
                     AVRDTVMNEIDVKEGNIIGIAEGNLLSAGDYVDEVTSNLIEKLVDEDTAIITLFFGED
                     VTESQANELRTSLEEKFEDVDVELYYGGQPLYYYLISVE"
     gene            6777..8834
                     /gene="recG"
                     /locus_tag="ACOIAF_000362"
     CDS             6777..8834
                     /gene="recG"
                     /locus_tag="ACOIAF_000362"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HGZ2667397.1"
                     /translation="MDLYKDVQYVKGIGPKKADKLNKLGIFTLKDLLYYFPRQFEDRN
                     NLKKIAQLEDGEKVTIKAVISSINTFSPKEGMTLTKIDVKDETGSAKLVFFNKSYIKN
                     TFRPGDSILVFGKVKKKFNNLELTSCELEYLTNSPKNTCRFMPVYQLTYGVTNKEIMS
                     IIRTVLEDKELIIQEYMPQRIIEKYRLCSIDFAVRNIHSPSSKESLKIALYRIVFEEL
                     LILQLGLFVFKSGRNKEDGIKFETSKDLKKIISALPFKLTKAQNRALDEIIQDMNLEK
                     IMNRLVQGDVGSGKTVVALLALANCVLNGYQGALMAPTEILAGQHYISLTESLKDFGI
                     NVGLLIGSLTKKQKDTVLEQIKNNEIDILIGTHALIEDKVEFNNIGLVITDEQHRFGV
                     MQRSKLSLKGANPDILVMTATPIPRTLALILYGDLDISIIDELPPGRQPIETIAIEKS
                     KRDRAYNNLVRREVESGRQVYIVCPLVEESEAIEAKSAVELVEELRAEYFHDLRLGLL
                     HGKMKSSEKDEVMRLFKDKEIDILVSTTVIEVGVNVPNATLMIIENAERFGLAQLHQL
                     RGRVGRGSHKSYCVLIYDSKTDVCRQRMAIMEETNDGFKISEKDLEIRGPGEFFGTRQ
                     HGLPELKVANLFKHIKILKLAQQEARYILGEDNNLQLKENMALKKEIIDKFKDTLKEI
                     SLN"
     gene            8893..9447
                     /gene="rsmD"
                     /locus_tag="ACOIAF_000363"
     CDS             8893..9447
                     /gene="rsmD"
                     /locus_tag="ACOIAF_000363"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HGZ2667398.1"
                     /translation="MRVISGKARGLKLNTPKNEDVRPTTDRVKESLFNMINSYIMESE
                     VLDLFAGTGSLGIECLSRGAKSCTFVDISKESIDIVKSNIKKARVESESIILNLDFKT
                     AIDKLKLQNSKFDIIFMDPPYYKNMFIEAIEKIDNSNLLNEDGIIVVEHDTNDLFPDK
                     ICKLEKTRDKKYGNTTLTFYKMEA"
     gene            9449..9946
                     /gene="coaD"
                     /locus_tag="ACOIAF_000364"
     CDS             9449..9946
                     /gene="coaD"
                     /locus_tag="ACOIAF_000364"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HGZ2667399.1"
                     /translation="MENKPRKAIFAGSFDPITNGHLDIICRASKLFDELQIGVLNNPN
                     KKGLFSFDERVKLIEKSTSHLNNIKVVTFDGLLINYCQENGIGALVRGVRSGADVDYE
                     LQMAHMNRELNPDIETIILPSCTKYSFISSSLIKEVLLFDADIKNLVPKIVLEELKKK
                     TSGGN"
     gene            9950..10474
                     /locus_tag="ACOIAF_000365"
     CDS             9950..10474
                     /locus_tag="ACOIAF_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667400.1"
                     /translation="MKIDLEMIELFEQLEEIFRSASTIPFSHKCTVDKDEVLAIINDI
                     RTLIPEEVTQAVWINKERNKIISQAKQDATNIVEQAKKEAERIQQEYQDNIEELKKNS
                     EDVVKAYVESSEPVVQADQRAKDIVDRAERIANEIRIGSIEYAEDVLSSVEYNLREIL
                     EEVKRDKAELRPKK"
     gene            10818..12737
                     /locus_tag="ACOIAF_000366"
     CDS             10818..12737
                     /locus_tag="ACOIAF_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HGZ2667401.1"
                     /translation="MKERAKKILDFIIKNNNVTSQELQEKFNVSKRTIYYDILAINEQ
                     LGKSGNIKNVKHKFIFEGNLCDARKIISTEEDKFLDSDYRKTFILNKILLGEKISIEK
                     LTNEMLLSKNTVVQTITDVKKYLQTMGLRLEYKGKYKIIGDEYVIRELFLIIVQENVL
                     EINSISEEVSSFDTKGHIKLTDYSLLNLTKFVEFLNKRIRDGKTLYSYKYLNEAKKIS
                     YFSNCKELLCEEANENEQAYICTYISSLPSLNSEVKEDVVEEYVDKLIDKFEVNTAIK
                     LESKHEFKKNILRHLHSSYNRIRFKFPIRNPMLDETKYKHESLYKIIKSIIENEEEFP
                     VFEGIREEEIGFIAAYFGGYLRGSRDNGLRRNKVLLVCPNGLMVSKSLEIQLYKYIPT
                     IEIVGIVSIKQLKEVNVYYDYIITTIDIQNVNNVIVVNPLLTSSDVQLLMNKLISVKE
                     NEKYFNLELIIQAIRKNGVINNEEALKADLLNIIHKIDEGEMYQPMLKELINAERVNI
                     IKNVRDWKEAIKIASKPLLEDNSIEELYIENMIKSVEKYGPYIVLADRFALPHASSKE
                     GVNKLAMSLLIVEDEVDLLGKPVNIFMVLAAVDNTTHIRALASLSEMMYEEENVKLII
                     NGDKSSILELINKQN"
     gene            12750..13037
                     /locus_tag="ACOIAF_000367"
     CDS             12750..13037
                     /locus_tag="ACOIAF_000367"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGZ2667402.1"
                     /translation="MKILAVCGFGVGSSMVLKMTIQKALKQLGIECEVENTDINSARG
                     ADCDCIFTSQELLNELKGTCNVDVYPVKKYMDLAEVTEVVQTFLSDKNDKK"
     gene            13170..14429
                     /locus_tag="ACOIAF_000368"
     CDS             13170..14429
                     /locus_tag="ACOIAF_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HGZ2667403.1"
                     /translation="MLDFISNNILRNPSMLIGLIAAFGLVLQKKKASDVIKGSLLAAF
                     GIIIMETGTGMLVSSIAPINGAFQSISGGAVTKGLSDVTFTASYGGIIGLAMFAGLAI
                     HLMIARFTPIKTIFLTGHMLYWFPFIFVASGVEAGLNGGVLIAFAAVLSALYWSIMPW
                     LLRKYVFDVIGDDSFTLGHPSGCLALISGFVAKLVGNKEKSTEDINIPQSLSFFREIS
                     ISGGMAVFLMFIVVGIVIPGAFKDSNEFIGTYAIKQGLSFGAGLIIMLHGVRMLINQI
                     MPAFQGISEKVVPNALPAFDCPILFNYKPNAVLIGFLVAMLTSTAMILICNYFNIFNL
                     LLIPLVITSFFECGAAAVIGESQGGIRGAIIGTAIAAIFMVVLVGISAVIYSGTIQNW
                     ILIFGGNDLSLFGIISRFIGSLFGGIL"
     gene            14432..15172
                     /locus_tag="ACOIAF_000369"
     CDS             14432..15172
                     /locus_tag="ACOIAF_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HGZ2667404.1"
                     /translation="MKFKYIDEIRRLIDVIENEEKVNMEKAVQILVEAIESKNAIFSF
                     GASHAGILTEELFYRAGGCVLINPVFAKSLMLDTAPITHTSKMERLEGYGTALADKTP
                     IKKGDVVIVHSVSGRNPVSIEFAMKAKENKAIVICITNLSYSKSVSSRHSSGKKLYEV
                     CDLVIDNHGQKGDACVEIDGLNQKVSPTSTVIATTVMNSIIAQATQELVNKGLKNPPI
                     FYSANIDGGDELNKKIFDEYKSVIHYEY"
     gene            15495..16805
                     /locus_tag="ACOIAF_000370"
     CDS             15495..16805
                     /locus_tag="ACOIAF_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HGZ2667405.1"
                     /translation="MKKENKMEGIKVGMLKKLCSLAMVAIMTTTLITGCSSGNGKDSK
                     KDGEKEKLVLWMPPVENNMKEVWDPILDKFEEKNNCEVDLQIIPWDNYSEKFATAISA
                     GEGPDVGYMYAEMYPQFIESGAVEDLTNYATKEDKEQSIYIKTSEMMGGMYGMPFQAA
                     NPGVLYYNKDILEKLGEKPPKTWEDFKRICKKATQDTDGDGKIDQWGLAQGWGAKTFG
                     NLNWNWYPYLWQAGGDIFNDDLKSVKFNDKSGLEAANFLKELQAYVPEDSLSKDSNEM
                     IESVFGPGKAAFTIMLSSAATSVFDESFPDLNWGFVTGLEDKKAATFGAVDHLSLMSS
                     AKNKELAYKLIQHMLSVESMTEFHKAIPRAPITKGEPYQGDERFKEMVENDKNVYRPL
                     VVGPHGVEIYEYLWKELQTMISGDKTPKQALDDAAKYSNDLLAQ"
     gene            16903..17820
                     /locus_tag="ACOIAF_000371"
     CDS             16903..17820
                     /locus_tag="ACOIAF_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGZ2667406.1"
                     /translation="MENLVREFEENSKRKKTKLNIKPYMYILPLGIILVAFYVIPIIM
                     SIYFSFTKYNIISPATFIGLENYKKLFTDETLKVSIINTIKFTVVVVPCQTILSLILA
                     VWITGKGNSKIASFAKGAIFIPVLSSMVLIGMVWRALLNGEGSIIYQVLGAFGIESSK
                     LLGDSKTALPTLMFISMWKNIGYFMVIYISAIMNLPKHCYEVAKVDGATKLQEFIKIT
                     VPLLKPTTIMVVFLGSIWSLQVFDLIYTVTGGGPGISTMSIVMHAFNLNFKNFNSGYA
                     MTVANVLFLLIAVVSILQNKLVRRDNSDF"
     gene            17836..18645
                     /locus_tag="ACOIAF_000372"
     CDS             17836..18645
                     /locus_tag="ACOIAF_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGZ2667407.1"
                     /translation="MKTARRLLSDGLLLFIACASLVPFIYMLIISLKITYNSYSLDIS
                     FSTVTLQNYIDIFTKKGFAQYFFNTAIVSFSGVLLNLVFSTLAGYSFAKMDFKGSDKL
                     FLFMIMTLIIPSQVTMIPLYIIMKHLGWINSYLALIMPIPTAFGVFIMRQAILGVPKE
                     LLESAKIDGCSDFRILIQIVLPLIKPALITLAIFTFMGAWNEFMWPLIATTKDAMRTL
                     TVGLSTLKTFQITNYGQMMAGATITFLPPFIFYLILQSKFVEGVSLSGIKG"
     gene            18703..20451
                     /locus_tag="ACOIAF_000373"
     CDS             18703..20451
                     /locus_tag="ACOIAF_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HGZ2667408.1"
                     /translation="MEISYVLKDCTFKHNKYSVIEAETWKDKDLDIVVAKGEKFAFQI
                     MLKATEEFNCTIDKSSAISWKGLGNRVRLALNVPNSLENNFSINILGYVQDDTKAFVN
                     DAILRDKDVLVEQYLPQTFWIEGQVPEDFEENNLDIGIDIFKSFGYEDEEKVCTIDVP
                     VKVRNVVLKPLDESKFFLDLWQHPSCLARMYKVELWSDIHFEIVDNYLKELASLGEKV
                     ATVIVSDYPWAGQSCYKVYKNPSNLYEYNMVSVSKGLDGKIKCNFESMDRYISIADKY
                     KMAKEIDLFGLLGNWCAGEFGNPVEGYKDPIRVRYFDEKDKVFKFINNTNDLKEYIGL
                     VLNHLIECGLWDRVRIIADEPNNPEVVKECIEFINSTVGTHQVKYKSATHDQNFLDRA
                     KDEIDDMSINLKLTIQNYKDIESLKKKINDKGGILTWFVCCFPEKPNSFLSSPFVENR
                     IIGWYTYYFGLDGFLRWDYNLWTEDPWKDSSYKFPIWKAGDMFFVYPGKDLKPVRSVR
                     MENLRFGIQDFELFTMLEKEKGREYIEVELMQELLNKKENAEIKGFGDIELGYSLDNW
                     GYDKVKKYVLELLDRV"
     gene            20755..21735
                     /locus_tag="ACOIAF_000374"
     CDS             20755..21735
                     /locus_tag="ACOIAF_000374"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - obsolete organic substance
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HGZ2667409.1"
                     /translation="MFFLSISFIGFYLVIRDNSDTVKMQRGINIGNALESPKDFPWDV
                     KMSNKFFDDIKDAGFDTVRIPVRFSDYTSDSDNFKIDEEFFKKIDKYVDYALDKDLIV
                     VLDLHHFEEIMKEPRVHKEKFLKIWQQIANRYQKYDKKLVFELLNEPKENLYSQLLNE
                     YIEEAIKIIRKTNPKRTIIVGPYNFYQIDYLNELNIPKDSNIVVSFHYYEPNDFAFQG
                     NIYHKGFEHLSNITWEGTNEQMDYLKKRFDTVENWANKNKVKIFLGEFGVTKEAPETS
                     RRAWVKAVREEAEKRNFSWAYWELASGFGIYNQIEGTWDREILSALIEKR"
     gene            21740..24046
                     /locus_tag="ACOIAF_000375"
     CDS             21740..24046
                     /locus_tag="ACOIAF_000375"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HGZ2667410.1"
                     /translation="MKKNSKKILITLTIITNIVYILWRIFYTVPKEEGMFALICAIIL
                     LFVEIMGMMEMFVHYYGMSNIEYPEKPIISEELYPHVDVFIATYNESVDLVRKTVNGC
                     IHMQYPDKKKVHIYICDDGNREEMCILAEKMGVNYITRTEREGAKAGNLNNAMQHTNS
                     PLIATFDADMIPMHDFLIATVPYFLKNEQAKKDGEKEEYEKVGFVQTPQSFYNPDLFQ
                     FNLHSEGRIPNEQDYFYRDIQLARNRTNSVIYGGSNTVISREALEEVDGFYTYSITED
                     FATGILIQSKGYRCYAIPEVHASGLSPTDLKSLIKQRERWARGCIQTGRRLNILFRRG
                     LGFWQKISYISSITYWYASIKRFVYIMAPILFSVFNVIVVKCTLLQVLVFWLPMYILS
                     SLSLKIFSQNIRNTRWTNIYETIMFQSLMPAVILETFAISKNKFSVTNKSKLEENRMY
                     KFLQGIPYFIYMVLSIIGILKMFVAIFKMSSMTYSVVLFWLIGNLFNLVMATLFISGR
                     QQLRKSERYIAEIDFKLKQNSYVLSSKTIDISENGFAFLLENPEYISPEEEFEVEFRE
                     KSGNEMYIANMKAKIVNVVEVNSKWKYAAYITHIEDSEIDNWMCIVHDRIPTLPMTIS
                     NQLGFFDDLQINVKKRIEKTRTLSRRSPRINMNFQMDIKNTGKLRIVNFNYQYVLLNF
                     ENKSIYPKEIALEINENIVLECDLCEGKIDERGILYKVNNIDSIMQNFFLRDEMMDWI
                     LQNKTILVSKPSEKKEKSIDEFEPMEYI"
     gene            24057..25133
                     /locus_tag="ACOIAF_000376"
     CDS             24057..25133
                     /locus_tag="ACOIAF_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667411.1"
                     /translation="MKTLKVFFCSTKYLILVFIFFINTTFISSATSDKILDLSFKKIE
                     TDLSSKITYEDTGVRIETDSSKSDKERYLYIYQNIKENWSMYNNFYIEIQNKNKSSQR
                     INLSIQSKNMLEFRLKEGSEVFLEGKNIIYSDKIKEGCIEVPGEFEGKIYVNFNSLIN
                     EESNVVLDSNMLSNIVSWGITFIPSDEEHNIVIIKKISLLSEEKLRFLNNIKIIGDEE
                     VQIPVLGQSISQYEVLGLKSDSKIKYSLMGKQDNVSISQKGKLTLSNKAKPGQIILQV
                     NVDDKFKIGKKITLTESWSVNKKDKDGVPYTLVYPEQSPTVQDMKKINFMNNIITFVR
                     ILFVSLVIICFGIYLYWKKCSKTK"
     gene            25151..26569
                     /locus_tag="ACOIAF_000377"
     CDS             25151..26569
                     /locus_tag="ACOIAF_000377"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HGZ2667412.1"
                     /translation="MLFSSLIFLFYFLPITLVLYYVFRFNRTIQNMILLAVSLFFYAW
                     GEPKFVVIMIVSIIMNYIFGLLVDRYRESKIKVKVFLVLMCAYNIGVLFIFKYLAFAL
                     RNISELISTELTIPSIVLPIGISFFTFQGMSYVIDVYRRHGEVQKNPFYVGLYIAFFP
                     QLIAGPIVRYESVAEQILNRKETWNKFSIGTCRFITGLGKKVLISNNMAIVADYIYTM
                     NSQGEIAASLAWLGSIAYTLQIFFDFSAYSDMAIGLGLMFGFKFEENFNYPYISKSIS
                     EFWRRWHISLGMWFKSYIYFPLGGSRVANKDIMIRNMFIVWLFTGIWHGAEWTFVIWG
                     ILNFVFLIIERFIMFEKIENHNFIKHIYALLVVNFGWVLFRSPNLKEAYNYFKAMFGG
                     NGAIWSDYTYMFLKEYWVFFIFAFIFSVPIAKKINKFVVEQAKYSMIFNVFYPISAIL
                     LFFISVTYLVTGSYNPFIYFNF"
     gene            26584..27792
                     /locus_tag="ACOIAF_000378"
     CDS             26584..27792
                     /locus_tag="ACOIAF_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667413.1"
                     /translation="MNRFFKVRLITAIMFLIILFAFSIFNVCYSGKDTIQEIKNSIAE
                     KSSLKDTISSIDNTVNDKVIYKNTFIETYGYLQKLMGKNEFSKFSVVKDTSGKMHYTY
                     FAKAPNPVGVIADRVRKFSDEMEKQNTKLVYLMTPDKYIKGVTKFPKGIPYSYSNETA
                     DNFLNQLKKYNVDYIDFRENILKSGIPKDDLFFKTDHHWKVETSFWAFGELVEQLNKK
                     YNMNLDENHYYRDKDNYNSIVYPKSFLGSMGRKTGILYGGIDDFTLIYPKFKTNYTYY
                     TDSKSQKFELKGRFEESLILSYPFNADLNLMDGQSDKYFTYLLGNRPLVKIKNIENPD
                     GLKVLFVKDSLIVPTAAFFSSVCSSIDMIDPRYYDGDILEYAKSHDYDFVFISVYPQN
                     LTKEFFPFCE"
     gene            complement(27866..29254)
                     /locus_tag="ACOIAF_000379"
     CDS             complement(27866..29254)
                     /locus_tag="ACOIAF_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HGZ2667414.1"
                     /translation="MESSFLSQFLMITDFKTIFFIILLLGTFFIVRQFEKKKIKFSLR
                     TIYATVLGLILGIIIQAFAGFPNDTTQVIWLQEVSKWYSLFGSGFMDLLKMLVVPLVF
                     LSIIKVIINMKDNNLGTLTFKSLGMLLATTAIAAIVGIIVANIMKLGVGANMPTLVEN
                     QELKEINSLVDTLRGLLPSNPVMAMANGNIVATIIFATFIATSIKRLSKKHFETIKPF
                     IDFIEASYKIIVSVSMTVIKFMPYAVVALLANTITSQGISSIVSVLQFILALYISVAI
                     LFIIHLIFITLNGLNPITYIKNSTEALLLAFTSRSSLGTLPVTIESLVQNHGVDEGVS
                     SFTASLGANMGMNGCAGIYPALMAVTLANMAGVNMDMSFYVMLLIVITISSFGIAGLP
                     GTATMAVSVVISGVGLGSYFPLVGGILAIDPILDMGRTMINVNGAMVSSITVGKSLGK
                     IDKEVFSKKNIS"
     gene            29595..30929
                     /locus_tag="ACOIAF_000380"
     CDS             29595..30929
                     /locus_tag="ACOIAF_000380"
                     /EC_number="1.8.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HGZ2667415.1"
                     /translation="MRVIIIGGVAAGMSAAAKLKRIKPEYEVVVYEKTEIVSFGACGL
                     PYFVGGFFNDADELLARTPEKLREAGIDLNIFREVVEVDSESKKIKVKNIKTDEIYED
                     YYDKLMIATGARSIMPSIKNIKLKNVSTLKSLYDGEYLKKLLSNEDNKRVTIIGAGFI
                     GLEAVEACKKLGKDVDVIQLEDRILPQVFDKEMTDVLEEEINRHNVNLHLDEMVVELC
                     GEDKVEKVITNKGEIDTDVVIIATGVRPNTEFLSNTNIKMLKNGAIVVDEYGRTSVED
                     IYSAGDCATIKNIVSNENVYVPLATGANKLGRIVGENLAGREVSYQGSLSSSCIKIMD
                     MEAASTGITERQAKDLGINVKSKFISDYNQTHYYPGRNKIYVKLIYDADTKVILGGQV
                     AGFKDAVQRANVLAAAIFGKMTTSQLGMLDLCYAPPFARTWDVLNVAGNISK"
     gene            complement(31071..31988)
                     /locus_tag="ACOIAF_000381"
     CDS             complement(31071..31988)
                     /locus_tag="ACOIAF_000381"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HGZ2667416.1"
                     /translation="MIDLTLLGCGGNVPMPNRFLSSVFINYKGRKILIDCGEGTQVSM
                     KLKKCGFKDIDLICITHLHGDHIFGLLGLLSTIGNSGRTSDLTIVGPVGIVDCIRSMR
                     NLVEYVPYTLKIIENPQGNFSLDNKVLRNLEISTISLEHSIECLGYSFNFRRNPKFDI
                     DKATKNEVPKILWNKLQEGQNIVLDSKQYTPDMVLGELRKGVKISLTTDTRPIESIPD
                     FIKDSDLFICEAMYGDDLDISKAVRNKHMTFREAANLAKLGNVKQLLLTHFSPSLDIP
                     SMYLENATNVFENTILGEDRLSLRLNFDE"
     gene            32196..33080
                     /locus_tag="ACOIAF_000382"
     CDS             32196..33080
                     /locus_tag="ACOIAF_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4846 domain-containing protein"
                     /protein_id="HGZ2667417.1"
                     /translation="MKVLKKITILLLITLTLVGCSNGEEKKVVEGSKKTQNSKKDEQP
                     IIVHKDANILKDRYEPPKGFKRVDVEKGSFESFLRNTKLKNYGEKVKYYDGRTKESKG
                     IYDSVFDVDIGNRDLHQCADAIMLMRAEYLYQNKLYDEISFDFVDGFKAKYSKWAQGY
                     RISVKDSGSSWYKATEYSTSYESFRKFMDIVFAYSGTLSLEKELEPVKVEDMKIGDVF
                     IVGGSPGHASIIMDMAENEKTGEKVFMIAQSYMPAQQTQLLINRKNPNLSPWYSLDFE
                     GDLTIPQWTFKRDQLKRF"
     gene            33293..35134
                     /locus_tag="ACOIAF_000383"
     CDS             33293..35134
                     /locus_tag="ACOIAF_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HGZ2667418.1"
                     /translation="MKIRRYLILVIIILITTSQSFISYGLERFEEITIFHTNDIHGRY
                     AEGDDHIQIGNLATLKKETPNSILVDAGDCLHGLPIVNMDRGKSAIELIKCAGYDYIT
                     PGNHDFNYGKDRLFELSKSANLGENTLKFLSSNIFENGKKVFDSNDIKEIDGIKIGFF
                     GLSTQETKSKTGFKNVEGLEFKNPIESATEQVNELKGKGANVIVAICHIGTNSLSKPN
                     SIDIANEVQGIDLIIDGHSHTKFENGKQVGDTLIVSAGQYLENIGEIKLTLDTNDVEN
                     IKIENKSVRLIGKEEALKYKLDSAVSKKVDEIKEQQEKILDKVIGVTTNTLDGSYENV
                     RTKETNLGNLISDILLDKTKADISLFNGGNIRDTIEKGNITRRDIVDVFPFSNTIVTK
                     ELTGAQIKDVLEHGVKLYPEKSSAFLQVGGISYYFNPKQKEGEKITNIQKEGKPLDLN
                     KKYVVATNDYIASGGDEFPCFSKEPVLKEFGNLESAVIEYIEYKKEISKNVDGRIGIK
                     SKDELISKNHESDLILSENEKNINDNEENLSNNIKSSDSDKNKSLDKTSARESSNLGI
                     SKREAKEKSPKTGDLGFSNSIIIFIVSSTLICLLNFNQKELKDKKSK"
     gene            35439..36146
                     /locus_tag="ACOIAF_000384"
     CDS             35439..36146
                     /locus_tag="ACOIAF_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGZ2667419.1"
                     /translation="MNNILLLEDDKSLNRGISFKLKKEGYNVFSAFGIEEAKNIFAKE
                     EICLIISDIGLPDGSGFDFCEEIRKKSDVYIIMLTALDEEVDIVTGYDLGADDYITKP
                     FSLMVLISKVNALMKRVNTVKNYTLLVCDDLFFYYIENKLIIRTDDKEEEIILSKTET
                     KLLKYLMENSMQTLTKEQLLESLWDSSGNFVDDNTIAVNIRRLRQKVEKNPSEPKYIK
                     TVRGVGYMWGERSIKKC"
     gene            36140..37450
                     /locus_tag="ACOIAF_000385"
     CDS             36140..37450
                     /locus_tag="ACOIAF_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HGZ2667420.1"
                     /translation="MLKNMNLKDGLSVKDDYIMRRVIVYSVLSVLIVTLVFILFEYSR
                     LHKLYEHQIKNTQNIAGILIEKYPNDEIDIVNSIYNSDYSKVSTGEKAFKKFGYGLEN
                     KMSNDKNFSLYLKSFFKESFFIFLMMFIVVIGIIYYFIRYINKRLSKIYFIVDKMSQE
                     NYLKDTTESEETGYLVEYNEYFKEGIFSKINNCLYELNRSLKIKFIKLEKEKESVKSL
                     VTDISHQLKTPLASLKLYNTLLIEEELEEDEANEFLLTNKASINKLENLINSLVNISR
                     LEISMINIKKEDNDIKNTILNAVKSVTPKAKSKSITLNLNEFNSRIIPHDKKWTEESI
                     FNVLDNAVKYTQVNGEINISVEETANFFKIIIEDNGIGIEKNEFNNIFKRFYRGSADE
                     IESVEGSGVGLYLSRKILEEQGGNIIVSSKVGIGSKFSLFLTTM"
     gene            37567..38250
                     /locus_tag="ACOIAF_000386"
     CDS             37567..38250
                     /locus_tag="ACOIAF_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2667421.1"
                     /translation="MKILYTENLSKHYGKGESLVRALDNVDLEINEGEFVAIIGKSGS
                     GKSTLLHMIGGLDIPTSGKVYIDNKNIFTLKEEELAVFRRRKIGFIFQSYNLIPSLNV
                     WENVVLPIGLDGREVDESFIKELLKSLGLENKHDVLPNTLSGGQQQRVAIARALATRP
                     AIILADEPTGNLDSKTSDEVMSILKSMSKKYSQTLVMITHDDSIAQMADRVIFIEDGR
                     VSKVGDKND"
     gene            38243..40630
                     /locus_tag="ACOIAF_000387"
     CDS             38243..40630
                     /locus_tag="ACOIAF_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HGZ2667422.1"
                     /translation="MINTTKIAKSNLKQNKSKSILIIITIILSTLMLSSIGIYIVDAG
                     AYQKENTIKYSGNYQGILANVDEKQADILSNHADVELTGEMNGVGVEKLEDDSNISLA
                     YMNEDALKLNSFEFIKGKLPSKENEIVLDSGALKALGYKNKDLGEKIKISYDDYKNDK
                     KIEKEFIISGILKTSEISEAGKYYYAIISESYMRNTRDMSQEDFNIYVKFNDKSNLSI
                     EQAKEKLDKIANDIGLDTINTAVNENYINALKPDMETIMGGVFVGLVIVLSSILVIYN
                     IFYISIVTKVQEFGKLRAIGATKKQIKNIVFKEGFILAGIAIPIGIILGYVLANIIIK
                     SFMDIDVKSSRLPVILSVVVISFISVVLSLLKPMKVASKVSIVDAVRYTGNKISNKSK
                     RKGYKNINLKRLSHANLERNKKRTYMTLASLILSGTIFITVSTALESFDAEKMAREHF
                     PYDIEVRLSGYEMNSDKNPKDNLNILQMDNPLSKDFFNQIKNMEGIKRIESARSVKIG
                     MEDYDVEFKYDLLQSINENDVKSLNKNIIDGKINLERLQTGDEIVITHVDTAKEMGVK
                     AGDKIRLTLYDGDKKIKKEFKVQAIAMGVPSFGIGKDFIDRTLKYDSTSSLGIYTEEG
                     KYQEIKDSIKKIAKNNGFLETDFIDSRIESNKATISFIKIMGYTLTGIIGVIGFMNLV
                     NTMITSIVTRKKELGMLQAIGLTNKQLVKMLNSEAMYYTSGMMIGSILFGGILGYIAV
                     IFLKKTGLSYATYSLPIVPILLMIVCILIAQFITTYLIGRSFNKESLIDRVRYSE"
     gene            40910..42052
                     /locus_tag="ACOIAF_000388"
     CDS             40910..42052
                     /locus_tag="ACOIAF_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HGZ2667423.1"
                     /translation="MNKKINYAPGPTETRENVRLARAEKTTNPDIDIDFVEFYKKTCE
                     KFGNIIGTKNDVYILSGEGILGLEAACASLTEKGDRVLVIDNGIYGEGFKDFVTMYGG
                     EYVLFSSEYTKSIDIDELKAFLDEDSNFKYATVVHCDTPTGVLNDVSKICPLLKKYGI
                     LTVVDSVAGMVGERLSVDESKIDIILGGSQKAISAPAGLTIVGISQDAKNCIKNRKTD
                     VIGFYCNLNIWEGYYEKKYFPYTMPISDIMGLDKALDNILEEGIENVLNRHEKIAYSV
                     RKSIEEYGLELFLEEGYSNTVTAIKVPESIGALELTDYMLKNYNTLVATSLNQYMDTI
                     LRIGHMGENANLNKIEHVLNVLDKSLSALGFKGNGNLLNLFNKYYF"
     gene            42071..42595
                     /locus_tag="ACOIAF_000389"
     CDS             42071..42595
                     /locus_tag="ACOIAF_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HGZ2667424.1"
                     /translation="MEVTAKQKFTTKDMVETSLLIALVFIATKFINIRLPISINGGLV
                     HLGTAMLFISAIVFGGKKGALSGAIGMSLFDLISGWTLWAPFTFIVRGVMGYLLGKIA
                     WANGNNGNNFLINVIGICVSSIWMLFGYYVTEVILYGNFIVPLTSIPGNLMQVLIGLI
                     IALPISKVLKNCIK"
     gene            42695..43513
                     /locus_tag="ACOIAF_000390"
     CDS             42695..43513
                     /locus_tag="ACOIAF_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6597 domain-containing transcriptional
                     factor"
                     /protein_id="HGZ2667425.1"
                     /translation="MLSVTRYSIKNVRLKPWVKFIWYFETKSNILLNNKLLPTDSIDI
                     ILNLSDVMEYKIENQDYTASNMHFNGIRDKHGFIIQHGNIRVIGISFYPFGLYPFLKI
                     PISEFNRQIVDLEVVSQLFAKKLEESLSPTQSVEKIVLYLEEVLLSILEEDLISNKYV
                     KLLNSFIYVNRYSNIKAFCDDTNINIKTLERICLKYTGYTPKILKRIYRFKMASNQLI
                     YNYKDDELFDFIYENEYYDQAHFIKEFKKFSGTSPIKFIGENKTIKENIKYSYL"
     gene            43591..43902
                     /locus_tag="ACOIAF_000391"
     CDS             43591..43902
                     /locus_tag="ACOIAF_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HGZ2667426.1"
                     /translation="MKIITEIVEFNVEENLSKEEFIEIVNDLELKFHSIQSGFIDTEL
                     LYDEKSQKWIMIQHWESIEDLKSASSKMFKDSSTEKFRKALKNQTVKMNIIPQIKSWK
                     L"
     gene            complement(44029..46032)
                     /locus_tag="ACOIAF_000392"
     CDS             complement(44029..46032)
                     /locus_tag="ACOIAF_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HGZ2667427.1"
                     /translation="MKSQKRFKILDIDVYLQPFESDIKKRMKHYEDMKSKTLKNYKDF
                     SSFANAHLYYGFHQTNDGFFYREWAPNADSLSLIGDFNNWNRKSHPLRKIGSGNWEIF
                     IPGQNSLPHKSEVKVQVTANGKTFDRIPLYIKRVIQKDFGGFNGQIWQPKTPFIWTDN
                     NFDLKNITSPLIYECHIGMSTESESIGTYNEFTEKILPKIKKAGYNTIQLMAIMEHPY
                     YASFGYQVSNFYAISSRFGTPEDLKNLINTAHSMGIAVLLDLVHSHAVKNTLEGINEF
                     DGSEHQFFHSGSKGNHPAWGTKLFNYGKPEVIHFLLSNIKFWLNEYHFDGFRFDGVTS
                     MLYHNHGLGVSFDSYEKYFSMNTDIEAITYLQFANELIKEIKPNSISIAEDMSGMPGM
                     CIPIKDGGIGFDYRLAMGVPDFWIKTISNLSDEDWDLGKMWYELTTRRPGEKNIGYCE
                     SHDQALVGDKTIIFWLADKEMYWNMEINSNNHVINRAISLIKLIKLITFSLAGEGYLN
                     FMGNEFGHPEWIDFPREGNNWSYKYARRQWSLSEDNNLKYKQLLNFDKSMLELTKYSD
                     IFIQNSHELIHMDNDKKILVYSKGKYLFIFNFHPNLIQSVDISKFQNTNYKTILNTDM
                     VEFGGNTKKENLRDYDVYFSSTPPSSKMPIASRTAIVLLDSSI"
     gene            complement(46185..46823)
                     /gene="recX"
                     /locus_tag="ACOIAF_000393"
     CDS             complement(46185..46823)
                     /gene="recX"
                     /locus_tag="ACOIAF_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HGZ2667428.1"
                     /translation="MGIITKIEQQKRNDDRVNIYVDDKFFIAIFKELVYTFNLKKGNE
                     INEEELKPILDDEMYIKAKNKALNILSHADQSEKKLKEKLSSEFDENIIERVIDFLKS
                     YNLVNDSVLAQKIVNTNVNLNKCGKNRIKQNLYNKGINRSTINEAVSELDKDTEFENA
                     MYLAKKRYERVKKEDKKKIYQKISQHLSYKGFDYDIIKRVLNKLLNFDEYDI"
     gene            47099..47788
                     /gene="nadD"
                     /locus_tag="ACOIAF_000394"
     CDS             47099..47788
                     /gene="nadD"
                     /locus_tag="ACOIAF_000394"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HGZ2667429.1"
                     /translation="MRSENLVKMAELKNTEKLEKFNRHKGKIKIGILGGTFDPIHYAH
                     LATAEFIRDKYDIDKIIFIPSGNPPHKLCITTDKYDRYNMTLLATESNEDFLVSKVEI
                     ERKKRTYTIDTLKYLKKKYKNADIYFITGADAICSVEEWKDVKKNFELATFIAATRPG
                     ISLLRSQETIEKLTKKYNADIITVYVPSLDISSTYIREQLNEGKSIRYLVPENVENYL
                     YENKLYQYGDD"
     gene            47789..48361
                     /gene="yqeK"
                     /locus_tag="ACOIAF_000395"
     CDS             47789..48361
                     /gene="yqeK"
                     /locus_tag="ACOIAF_000395"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HGZ2667430.1"
                     /translation="MNLENINQRLNQMLPVGRLSHSKNVAKCAEKLCEIYGCDKEKAY
                     LAGMIHDCAKYLSDKEIEDYVNKYEIYLDPIEDGNRSLSHSVIGAYICEYEFEVEDED
                     IINAIKYHTTGREDMSLLEKIIYIADLIEEGRKFPVVDTLRELAYGGKLDEALLTSFN
                     NTLMFVINKKEEIHPRTVMARNYLIKEKLL"
     gene            48478..48828
                     /gene="rsfS"
                     /locus_tag="ACOIAF_000396"
     CDS             48478..48828
                     /gene="rsfS"
                     /locus_tag="ACOIAF_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HGZ2667431.1"
                     /translation="MTVEQMTKIAYDAIEDKLGQDTVIINIGKVSSLCDYFIITTASS
                     QRQVKAIADNVEDELAKLGLEPRGKEGQGTQTWVLLDYGDIMVHVFNEENRGFYNLEK
                     LWKDAPYIDIDTLA"
     gene            49182..51602
                     /gene="leuS"
                     /locus_tag="ACOIAF_000397"
     CDS             49182..51602
                     /gene="leuS"
                     /locus_tag="ACOIAF_000397"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HGZ2667432.1"
                     /translation="MSVYNFKEVESKWQKIWKDNNQYKMDTAQTEKPNYYTLEMFPYP
                     SGKIHMGHVRNYSIGDVVARFKKMEGYNVLHPMGWDSFGLPAENAAIKHGIHPHKWTM
                     ENIEEMKEQLNLLGISYDWDKEVATSTPEYYRFTQEIFLKFLEHGLAYKKKSYVNWCP
                     SCETVLANEQVVQGACERCKATVLKKDLEQWYFKTTEFAEELLNDLDTLDGWPEKVKT
                     MQKNWIGKSTGADLVFDIDGTDKSMTVFTTRPDTTYGVTYMVLAPEHELVKELVAGTE
                     YEADVEAFVQKMHTMTEIERTAADVEKEGMFIGRYVINPLNGKKVPLWIANYVLVEYG
                     TGAIMAVPAHDERDRDFAEKYNLDIIDVITEDNKMINSEEFNGLDASEGFEGIIDKLE
                     KEGRGKRTINYRLRDWLVSRQRYWGCPIPVVYCDECGIVPVKKEDLPVLLPTDVEFTG
                     KGESPLTTSKQFMSTTCPHCGKPARREVDTMDTFVDSSWYFLRYVDSNNENEPFSKEL
                     VNRWHPVDQYIGGVEHAIMHLLYARWFVKAFKSMGMVDFNEPFKNLLTQGMVLMDGSK
                     MSKSKGNTVSPMDIIDEYGADTARLFVLFAAPPERDLDWSEQGVDGCFRFLNRVYRLV
                     DELADVVKKDVEFGELNSQDKDMRYTIHSTLKKVTADLSEKFGFNTAISALMELINDM
                     YKYKELDNINEAVIKEGVQTIVTIIAPFAPHLGEELWTMIGKEGSVFDIDWPKYDEKA
                     LVKDEIEVVVQVNGKVRGKLTVNSNISKDEMEKVALEDEKIKGLVEGKTIVKVVAVPK
                     KLVNIVVK"
     gene            52438..53106
                     /locus_tag="ACOIAF_000398"
     CDS             52438..53106
                     /locus_tag="ACOIAF_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HGZ2667433.1"
                     /translation="METVTNNQSGVEIQLSLFEAIKDLLLINPIFLLGIILIGIFLVV
                     IFKKNSDIPKVKTSILSLAMYYYLCLTLSHIVGIPTFGEYIRLSQLGETFFNPNINLI
                     PFSDGFSLSFILNILLFVPLGFLCPLISKTFERLRNTFLIGFGLSLFIETIQLFTLHR
                     ATDIDDLLTNVIGTIIGYFCFKLIAKLRIVKLHSDDDSMEQDYTVCIPIAIIIVALIL
                     GFFS"
     gene            complement(53424..53696)
                     /locus_tag="ACOIAF_000399"
     CDS             complement(53424..53696)
                     /locus_tag="ACOIAF_000399"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGZ2667434.1"
                     /translation="MSKHQFKSVSTFFTKKNVSQFYKKNNLKKIRIHDLRYTSATLLL
                     LGGTNMKVVSERLGHTDIKITMNRYSHVLKEMDKEASDNLSELLFK"
     gene            complement(54226..54348)
                     /locus_tag="ACOIAF_000400"
     CDS             complement(54226..54348)
                     /locus_tag="ACOIAF_000400"
                     /inference="COORDINATES: protein motif:HMM:NF026010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667435.1"
                     /translation="MTFVERCYKYIDMNKNNWSPYTVTNTKSWVKNYIEPFLRI"
     gene            54748..56118
                     /locus_tag="ACOIAF_000401"
     CDS             54748..56118
                     /locus_tag="ACOIAF_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp29"
                     /protein_id="HGZ2667436.1"
                     /translation="MKLKKLLSLGLAVSMFAAGPISANALDTIYTIQGKDKYETAALI
                     ADEQNYTTAILINADSTMADGLSASGLAGATNAPIFLTKKNNIPNATLKRVEKAKKVY
                     IIGGESSIDKATETLLKDKGIEAKRLQGSDRIKTSYNVAKEINSINKVNKVILTNAFK
                     GEPDAMSAAPVAVRDKAAIVLTDGKSVPFNTTGVESYVIGGTSSMSDKLVNDTNSTRL
                     GGVDRYDTNKKIVNKFYNGAKEFYIASGTDLVYALVASPMAKNNPIVLVDIGSNKDIL
                     KNAAKVTGIGNLSDKIMLECENTINNISLPSSNKNIICGLKIDFDSKHIIWQDKGSYL
                     ECYDDNLEYYTEKYKDPTVPINIENTSTQNLDVTKLKVYGEANSTLYMGQYAKIKMDF
                     NSFGVLKPGEKCTIYLDIISLDPSRSMTLTFSYVGDKENYKYEQGLASRILFYNPDGE
                     DFFEYK"
     gene            56477..56635
                     /locus_tag="ACOIAF_000402"
     CDS             56477..56635
                     /locus_tag="ACOIAF_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667437.1"
                     /translation="MDNFLQGILASLSASLIVYIASKLFRKRKKPLKAATKSGWEFDL
                     KIRFHKTK"
     regulatory      complement(57157..57254)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            57579..57863
                     /locus_tag="ACOIAF_000403"
     CDS             57579..57863
                     /locus_tag="ACOIAF_000403"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HGZ2667438.1"
                     /translation="MEKTYAWKHTTTITLLARLTKKGFFISQRIGKHVHYRVLIKEKE
                     YLKIEGKRIFGGLHNNPLSELISKLHDGEEITEEKIIEIGNWIKSWKDED"
     gene            complement(58052..59047)
                     /locus_tag="ACOIAF_000404"
     CDS             complement(58052..59047)
                     /locus_tag="ACOIAF_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HGZ2667439.1"
                     /translation="MSIKKKVAIVFTGGTISMTVDDKVGAAIPTLSGEQILSMVTNID
                     KVADVEVFNFDEIPGPHITPYRMMELKNYVNDLLARKDITGVVITHGTDSLEETAYFL
                     DLTIDNIKPVIVTGAMRSSSELGYDGSSNLSASVCTAVSKDAMGKGVLVVLNNEVLLA
                     SEATKTNTLSLNTFKSLTSGPLGIIDCNELVLTRDIVNRTIIDTDKVESKVALFKAYV
                     GENADFIRFAVDSGYKGIVIEAMGRGNIPPQMLSGVEYAREKGLPVVIVSRCHSGRVF
                     DSYGYFGSGRDLKNLGCIFGGDLPGQKARIKLILALGKTDNLDEIKDFFEKGIYC"
     gene            complement(59168..60796)
                     /gene="aspD"
                     /locus_tag="ACOIAF_000405"
     CDS             complement(59168..60796)
                     /gene="aspD"
                     /locus_tag="ACOIAF_000405"
                     /EC_number="4.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047688 - aspartate 4-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006531 - aspartate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 4-decarboxylase"
                     /protein_id="HGZ2667440.1"
                     /translation="MKNHTYKEIKNIYGKISPFEFKDKLIDIAKYTAKENNRELLDAG
                     RGNPNWTCSTAREAFFTFGHFAITETRSNWDLGHLAGMPQKKGIKERFFKFINENIDM
                     PGAYLARDIINFGINELGFDGDEFVHELADGIIGDNYPLPDRMLPHMEKIVHDYLVQE
                     MKYDISGKYGDVEIFAVEGATAAMCYIFDSLMANELLKKGDTIALMTPIFTPYLEIPN
                     LPRYDFKVVNINANEVDEKGAHTWQYTKKELEKLRDKSIKALFVVNPNNPASIAMDET
                     SCNNLIDVIENYNKDLMIISDDVYGTFVEDFSSLMSKLPYNTVGVYSYSKYFGVTGWR
                     LGTFALHKKNVFDKKINDLTGELKKSVDKRYSDMSLNPSSLSFMERVVADSRLVALNH
                     TAGLSTPQQVQMAFFSAFALIDKVDAYKKLNMSICHRRQKLLFEALELPINENKNNAA
                     YYTQFDIEEWAKLNYGEGLFKFISKNASPVDVLYKLANDYSVVLLSGNGFYGPEWSIR
                     ISLANLYDEAYTKIGKAIREILNGYIVEWQKIKK"
BASE COUNT        23620 a         6837 c        10244 g        20382 t
ORIGIN      
        1 aatacaattt tattagagta attgtattat atgagtgtaa atagtataat ttagaagaaa
       61 ttctataagg ggggatattt atgcacaaga gattgctcac gttgtttaaa ttattaaatg
      121 aaagtgatga taaaattact tgtaaaactt taagtaatca tttaaaagtg agtgagagaa
      181 ctataagaaa tgatattact tctataaatg gaactttaga gaaaaatggt gctattatta
      241 aaataaaaaa aggagaagga tattatatag atattcttaa tttagcatta taccaacagt
      301 atctagcatt aatatctgat gatattatgg attcaagtga aataccagat tctcctatag
      361 aaagaaatca atatatttta aaatatattc tctataacaa tacatatata aaactagaag
      421 atttagctaa tagcttgtat gtgagtaaat ttacaatact aaatgacata aaaagaataa
      481 agcctatttt gtcaaagtat aatttaatat tagtttctaa gccatattat ggagttaaag
      541 ttgaaggtaa ggaaatagat attcgaagat gtatttctaa taatatgata aataggaatt
      601 ttgaaaacta tataattgga attacagata gagaaataga attatttaat aatgtagatt
      661 taatagagct tcaaagggta gtgttaagtg aaattaataa atttaatatc aatttcctag
      721 attttaatct aaaaaacttt attatccatt tagctattac tataagtaga atacttgatg
      781 gttactgctt agataatgtt ttagatgttg ttttaacaga ttttcaatcg aatacaactg
      841 ttgaaaatat atttaattat attgagagta aatatactat aattatttca aaggcagaca
      901 gagtttatct atataatcat tttataacta aaagttctct tttggataac gtttccaata
      961 gggtagatac taaaattata gagtatgttg aagaaatttt agaagtaatc aacaatcaat
     1021 atacttttga ccttagaaat gatagtgtat tatttgatga tttagtactg cactttaaat
     1081 caattctcaa ttctaaatct tataatttaa ataaggtgaa tccccttata aatacaataa
     1141 agagtaatta tcctttagca tttgaaataa ctttaaatgc tatagaaaaa gtttttaaaa
     1201 atagtattta ctctttaaca gaagatgaaa ttggatatgt atccctgcat ataggtgctg
     1261 gaatagagag attttttcaa aataatatta agtgtaaaaa cgtagtatta gtatgtggtt
     1321 cagggtatgg ttcaagtagg ttattagagg ttcaattaaa caaagttttt catgataaaa
     1381 taaatatact tcaatgtcta tcatttaatc aatttttagc tagtgaattg tcagatgtag
     1441 atataataat atcgacaatt cctttaaatc atgatagtat tcctattgta ttagtggatt
     1501 taaaactatt aaaaaaagat atagagaata tatctaagtc aatcactaat aattcccata
     1561 tttatagtaa tcttttggac aatttctttg ataaaaattt atttattgtt aatcctaaaa
     1621 tcaaagataa agatgagctt ataaagttga tgtgcaacaa attaactcaa agtgaaattg
     1681 tatttccatc atttgctgaa tctgtatttt acagagaaag tttatcttct accaatatag
     1741 atgatttttt agctatacct catcctatgg aattaagctc tataagaact aagatttgta
     1801 tttccatatt gaatgaacca atatattgga gtgaagattc tactgttaaa ttaatcatga
     1861 tgttggctat aaataaagat gattatataa aaataaactc catttatgat atccttttaa
     1921 agataattca tgacaatgat attagagata gtatatctaa ttgtaatgat tttgataatt
     1981 ttctttccat aatcaagtct atagtataga tttttatatt acataagttt taaaaacttt
     2041 aataaaatat tttttgtttt tagcataaat agtataagta agttttatat aatttgttat
     2101 tattttgtat tgattataat tattatacaa taaagattct gtctcaaaat agagagagaa
     2161 acctgttttt cattgataaa atgataaaaa tatatttgat ttcatacaaa aaagagtgtc
     2221 tcatgaacta aaaagttcat tttgagacac cacctttatt attttatatt atttatctct
     2281 tgtatatatt actatagcat cccttaaaaa cttagctaat ccaggttgtt ctctatcata
     2341 atatgatgta aaacgttcat catatacata catctgtgct agagaggaat gagcttcttt
     2401 agagtatgtg ttccaagtgt aactcaacca tttatgatgt aattttgcaa cttcctgagc
     2461 cagttcacta gaggcatcac cagttttaaa agctttttta agtttactaa ttatttcgat
     2521 atttaggttt tgccaatttt cataatcttg cttagacata tttttaagtt ttttatttga
     2581 tgtatctata atatctttac catatttctc tctaatttca gttccataat ttttttcatt
     2641 ttcatcaatc atcttttctt taaaaccttc aaatttttct atatctgtca tagtgtattt
     2701 tccctccttc agagctatag ttttagttac attttctatt agtaaatcaa tttgctttct
     2761 tttggctagt aatttattat ggtgttcttt taacgcactt aaactatcga agtttggtga
     2821 atttataatg ttttttatat tttctaagtt aattcctaat tctctataaa aaagtatttg
     2881 ttgaagttta ttaacttcat tttgaccata tatacgataa cctgaagaat ttattttaag
     2941 tggcttaaga agacctattt catcataata tctaagtgtc ctagtactta taccagctag
     3001 tttacttaat ttttgaactg tatattccat aagtagtacc tcctataaat taaatataca
     3061 cttttacgca acgtaaatgt caatacatta aaatataaaa atttatatct tttaataaaa
     3121 atataaaaaa atatctttac tggatatttt acacacaact gtcaatactc actagtgaaa
     3181 attaaaaaat aaaatttata gactatcttt actaaaaaat agaaggtcta tagtctagga
     3241 ggaactgtat gaatttaaaa agaagcaatg aaaaacgaga agagtacaga agaatgtatt
     3301 cagataaaga aagtttttta agtttgattc aaaattttac aagtgtctca atagcaaaag
     3361 aattaactct taaaaatata gagttagaaa catcttttat atgtgaatat aaaggaaaag
     3421 aagttgatat aatttataag gtattttcta aaagcggcaa agtatcacat tatatagtgt
     3481 tagaatttca aacagaaatg gatactgaaa ttgtcccaag attgaaatcg tatagagaac
     3541 aaatttggaa aagttttatt atgaaaaaaa gtcttgaaga aatagaagat aaaaatttta
     3601 aacttcctaa agttatacca gttgttttat atagtggacc tgaaaggtta agtagaaaaa
     3661 aaggaatttt ggatattgta gaatctatat cggagttcag taataattcc agtatgaaaa
     3721 attaaaatta cataatgtgt ttattggtct aaaatggaaa actgtcgcat aaacttgaat
     3781 atgaaaatat ttatagatac aggttctatg tggaggtgtt ccatgagaaa taacaagtct
     3841 aaggtattgc taggaatact ttgtctagca acaggaatgg ctgtaattct gtcaatgttg
     3901 cttcctggct ggatttggtc agcacttacc gcattaatac ttattggatg tggagcatta
     3961 ttattttttt gttaaaatag ttttaactat aaagtgtggg gtgagtattg atgaaagtag
     4021 taacaataaa attaccaaaa tggatttcaa gtataatcct aaaatttatg agaaagagta
     4081 aaaatgatta aattaaaaag cctacttacg gaagtaggct tttaaacgtg gttactaagc
     4141 tctttcaatt ttaccagaac gtaaacatct agtacaaact tttactctct taggagctcc
     4201 atctataatc gctcttacgc ttcttaagtt agctgaccat gttcttttat tatgtttatt
     4261 tgagtgtgat acttggtttc cagaaacctt acctttacca catacgctac atacttttgc
     4321 catctacgca cacctcctta aaatgataac tatttaaaga ctaaaaacat atttatatta
     4381 tcataaataa caaaaatttg caatagaaat atatatttgc cttacacata ttgaagaatt
     4441 tcttattatc atataaaatt atatatataa ttttatataa agtaaatata tttggaaaat
     4501 aattattatt tttctttgaa tatatatact atagaagaaa ttttatccaa aaacttttct
     4561 aggtttaaat tataccatat ttatagtatg atatgattat aattttatat aataaataag
     4621 taaaaatagt aaaaaataaa ttaagaaatg tttttaataa aaataagctt aagggggtca
     4681 accatgagtg caaaggtaac gaatcaatat ggaaatatag aaatagataa tcaagtaata
     4741 gctcaagtcg tatatagagc tgcaatggaa agttatggct tagtaggttt agcatataag
     4801 tctaaaggaa tagtagagct attaaaaggc gagaacgcaa ctaaaggtgt aagagttgag
     4861 gaagtagaag aagatgccat agcaatcgaa ctttatgtta taatacaata tggtacaaat
     4921 atatctactg ttgccaataa tataatagat agagtaaaat atgtagttga aaaaatgact
     4981 ggcgtaagag ttactagaat tgatattaat gtacaaggaa taagagttaa gtaatttcat
     5041 aggaggaaac aaatgattca atatatagat ggtaaaaggt taagggagat gtttatttca
     5101 ggtgcaaaca atctccaaaa caataaagag ttagttgaca agttaaatgt attccctgta
     5161 ccagatggag atacaggaac taatatgtca ttaacaatat cttatgcctt aaaagaatta
     5221 gcaaaagttg aaaatgataa tatatcagat atcggaaaag cattatctaa aggttcttta
     5281 atgggagcta gaggaaactc aggagttata ctttcacaaa taatcagagg tatagctaaa
     5341 tctatagaag gaaaaagtaa attatcaaca gaagatttag caaaagcttt taaaaatggt
     5401 tcagacacag catataaagc tgtaataaaa ccaattgaag gaactatact tactgtagtt
     5461 agagaaagtg gagagtttgc aataaaaact gcgaaaaaag agaaagatgt agtaaagttt
     5521 ttaagtatgc ttgtgaagga atcaaattct tcattagaaa gaactccaga ccttctaaag
     5581 aatctaaaag aagctggtgt ggttgactct ggtggaaagg gtcttgtact tatatatgaa
     5641 ggaatgttag cttctataaa aggcgataac atagaaataa aaaatgttga cctagatact
     5701 aatatttcaa caagtatgga ttttgcaaag agtactacta gcacagatga tataaaatat
     5761 tgctattgta cagaatttat tttagaaagt tctaaggtag aagatactaa aataagagac
     5821 ataatgatgg cttatggtga tagcctagca gtagttggag atgatggagt cataaaggta
     5881 catgtacaca ctaatgaccc aggaaatgta cttcaagaag ctctaaagta cggtcaatta
     5941 ttaactataa aaatagaaaa tatgaagtta caacatgaaa atacattact ggatgtagaa
     6001 gaaaagaaag aaaatgatag tgaaccttta gaagaagaaa aagagtttgg gtttatagct
     6061 acttctatgg gtgaaggttt ggcaaatata tttaaagatt tcggagttga ccatataata
     6121 gaaggtggtc aaactatgaa tccaagtact gaagatttta tgaatgctat aaagcatata
     6181 aatgctaaaa atatatttat tttcccaaat aacagcaata taataatggc tgctaatcag
     6241 gcaaaagaat tgagtgataa aaatataata gttataccta caaaaaatac gcctcaagga
     6301 tttgctgcat tagtaacttt taatggagaa ttaagcgaag atgaaaataa agaagctatg
     6361 atgaatgctt taaactcagt taaatcaggt caagtaactt ttgctgttag ggatacagtg
     6421 atgaacgaaa ttgatgttaa agaaggtaat ataattggaa ttgcagaagg gaatttatta
     6481 tcagcaggag attatgtgga tgaagtaaca tcaaacctta ttgaaaagtt ggttgatgaa
     6541 gatactgcta taataacatt attctttggt gaagatgtta cagaatctca agcaaatgaa
     6601 ctacgtactt ctttagaaga aaagtttgaa gatgtagatg tagagttata ttatggagga
     6661 caacctcttt attattactt aatttcagtt gaataatgat tataagaacc tgtatgacca
     6721 agtagctttt ataaaattgg ttgtacagga tttttgtgta gtaggtgata tatttgttgg
     6781 atttatataa agatgttcaa tatgttaagg gaataggacc taaaaaagca gataaattaa
     6841 ataaactggg tatatttaca ctaaaagacc tattgtatta ttttccaaga caatttgaag
     6901 atagaaataa tctaaagaaa atagcacaat tagaagatgg tgagaaagtt acgataaaag
     6961 cagtaatatc aagtataaat acatttagcc caaaagaagg tatgacttta acaaagattg
     7021 atgtaaaaga tgagacaggc tcggctaaat tagtgttttt taataagagt tatataaaaa
     7081 atacttttcg tccaggtgat tcaatattag tttttgggaa ggttaagaaa aaatttaata
     7141 acttagaact tacatcatgt gaattagaat atcttactaa ttcacctaag aatacatgta
     7201 gatttatgcc tgtgtaccag cttacatacg gtgttacaaa taaagaaatt atgagtataa
     7261 ttagaacagt acttgaagat aaagaattaa ttatacaaga atatatgcca caaagaatta
     7321 tagaaaaata taggctatgt agtatagatt ttgcagttag aaacatacat tcaccaagta
     7381 gtaaggaatc attaaaaata gctttatata gaattgtatt tgaagaatta cttatactac
     7441 aactagggtt gtttgtattt aaaagtggca gaaataaaga agatggaata aagtttgaaa
     7501 caagtaaaga cttaaaaaaa ataataagtg ctttaccatt taaactgact aaagctcaaa
     7561 acagggcatt agatgaaatt atacaagata tgaatttaga gaaaataatg aatagattgg
     7621 tacaaggtga tgtagggagt ggtaaaacag tagtagctct cttagcactt gcaaattgtg
     7681 ttttaaatgg ttatcagggc gctttgatgg cacctacaga gatattagca gggcaacatt
     7741 atatttcact tactgagtct ctaaaagatt ttggaataaa tgtaggtcta ttaataggta
     7801 gtcttacaaa aaaacaaaag gatacagtat tggaacaaat aaagaataat gagattgata
     7861 tattaatagg tacacatgca ctaattgaag ataaggttga atttaataat attggtttag
     7921 ttataacaga tgaacaacat agatttggtg ttatgcaaag gagcaaactt tcattgaaag
     7981 gagcaaatcc tgatatatta gtaatgacag caacaccaat accaagaact ttagcactta
     8041 tactctatgg agatttagat atttctatta tagatgaact tcctccagga agacagccaa
     8101 tagaaacaat tgctattgaa aaaagtaagc gagatagagc atataataac cttgtaagaa
     8161 gagaggttga atcaggaaga caagtgtaca ttgtatgtcc cttggtagaa gaaagtgaag
     8221 ccatagaagc caaatcagca gttgaacttg ttgaggagtt aagagcggaa tattttcacg
     8281 acttaaggct tgggttgctt catggtaaaa tgaagtcaag tgaaaaagat gaagtaatga
     8341 gactttttaa agataaagaa atagatattt tagtttcaac aacagtaata gaagttggtg
     8401 tcaatgtgcc aaatgcaaca ttaatgataa tagaaaatgc agaaagattt ggacttgcac
     8461 aattacatca gcttagaggg cgagttggaa gaggtagtca taaatcgtat tgtgtactaa
     8521 tttatgattc taaaacagat gtatgtagac aaagaatggc tataatggaa gagaccaatg
     8581 atggttttaa gatttcagag aaggatttag aaataagagg acctggggaa ttttttggga
     8641 caaggcagca tggtcttcca gaacttaagg tagcaaactt atttaagcat ataaaaatac
     8701 tgaagttagc acagcaagaa gctcgatata tattgggtga agataataat ctacaattaa
     8761 aagaaaacat ggcactcaaa aaagaaatta tagataaatt taaagataca ttgaaagaaa
     8821 tttcattaaa ttaattttaa attgtgttaa aatattacta atgtaataat gtgctgaagg
     8881 gagattgtca tattgagagt aatttcagga aaagcgagag gattaaaact aaatactcca
     8941 aaaaatgaag atgtaaggcc aactacagat agagtaaaag agtctctatt taatatgata
     9001 aattcatata taatggaaag tgaagttctg gatttatttg ctgggactgg ttctttagga
     9061 atagagtgct tatctagggg tgccaagtca tgtacttttg tagatatcag taaggaaagc
     9121 atagatattg ttaagtccaa tataaaaaag gcaagagttg aaagtgaaag tattatttta
     9181 aaccttgatt ttaagactgc aatagataaa cttaaattac aaaatagtaa atttgacata
     9241 atttttatgg accctccgta ttataaaaat atgtttatag aagctattga aaaaattgat
     9301 aattctaatc tattgaacga agatggaata atagttgtag aacatgatac gaatgattta
     9361 tttccagata agatatgtaa attggagaaa actagagata agaaatatgg aaatactaca
     9421 ttaacttttt ataagatgga ggcataatat ggaaaataaa cctagaaaag ctatatttgc
     9481 aggaagtttt gacccaatta ctaatggaca tttagatatt atttgcaggg cttcgaagtt
     9541 atttgacgaa ttacaaatag gagtattaaa taatcctaat aaaaagggtc tatttagctt
     9601 tgatgaaaga gtaaaactta tagaaaaaag tacaagccat ttaaataata taaaagttgt
     9661 gacttttgat ggtttattaa taaattactg tcaggaaaat gggattggag cattagttag
     9721 aggtgtgaga agtggtgctg atgttgatta tgaacttcag atggcgcata tgaatagaga
     9781 gcttaatcca gatatagaga ctataatttt accaagttgt acaaaatatt catttataag
     9841 ttcatcttta ataaaagagg ttttgctctt tgatgctgat ataaaaaatt tggttccaaa
     9901 gattgttttg gaagaattaa agaaaaaaac tagtgggggt aattgatata tgaagataga
     9961 tttagaaatg attgaactat ttgagcagtt agaggagata tttagaagtg cttctacaat
    10021 ccctttctca cataaatgca cagtggataa agatgaggtt ttagcaataa ttaatgatat
    10081 aagaacttta ataccagaag aagtaacaca ggcagtatgg attaataaag aaagaaataa
    10141 aataataagt caagctaagc aagatgctac aaatatagtt gaacaagcga aaaaagaagc
    10201 tgagagaata caacaagaat atcaagataa tatagaggaa ttaaagaaaa attctgaaga
    10261 tgtagtaaag gcatatgtag aatccagtga acctgttgtt caagcagacc aaagagcaaa
    10321 agatatagtt gatagagctg agagaatagc caatgagata agaattgggt caattgaata
    10381 tgcagaggat gtgctttcta gtgttgagta taatttaaga gaaatattag aagaagtaaa
    10441 aagagataaa gctgaactta gacctaaaaa ataaatttta aataaataaa aatggagcta
    10501 tcacacaaaa aattccaatt tatgtgtgat agctctcttt taatatagca attttttgtt
    10561 atataattgt gctttttaga ttacttatat cttattttga tattataatg tagtttaatc
    10621 ttttatttgt tgaaatcaac ttatttattt gtataaataa tataggttat tttcaaattt
    10681 ttttaataat tttaccactg tcttctttta gattagagtt agactgtgca aaattataaa
    10741 ataatactaa atcatgtgca attatttggt atggcactac atgtagaaaa tatagataat
    10801 aaaattaagg aggtagtatg aaggaaaggg ctaaaaaaat attagatttt attattaaaa
    10861 ataacaatgt aacatcacaa gaactacaag aaaagtttaa tgtttcaaag agaactattt
    10921 attacgatat tctagctata aatgaacaac taggaaaaag tggtaatata aaaaatgtaa
    10981 aacacaaatt tatatttgaa ggtaatttat gtgatgcaag gaaaataatt tcaactgaag
    11041 aagataaatt tttagatagt gactacagaa aaacttttat attaaacaag atattacttg
    11101 gcgaaaaaat atcaatagaa aaactaacaa atgaaatgtt actttcaaaa aacacagtag
    11161 tacaaacaat aactgatgta aaaaaatacc ttcaaacaat ggggttaaga ttagagtata
    11221 agggaaagta caaaattata ggtgatgaat acgttattag agagctgttt ctgataattg
    11281 ttcaagaaaa tgtactggaa attaattcta taagtgagga agtaagtagt ttcgatacaa
    11341 aaggacatat aaaacttact gattattctt tattaaatct aactaagttt gtggaatttc
    11401 taaataaaag gataagagat gggaaaacat tatactcata taaatatcta aatgaagcta
    11461 aaaaaattag ttactttagt aattgtaaag aacttttatg tgaagaagct aatgaaaatg
    11521 aacaagccta tatatgcact tacataagct cacttcccag cttaaatagt gaagttaagg
    11581 aagatgttgt tgaagaatat gtagataaac ttatagataa gtttgaagta aatacagcaa
    11641 taaaattaga aagtaagcat gagtttaaga aaaatatttt aaggcacctc cactcttctt
    11701 acaacagaat acgatttaag tttcctatta gaaatccaat gttagatgag actaagtata
    11761 aacatgagtc actttataaa attataaaat caattataga aaatgaagaa gagtttcctg
    11821 tatttgaggg gataagagaa gaagaaatag gatttatagc agcgtatttt ggaggatatt
    11881 taagagggtc tagggataat ggtctaagaa gaaataaagt cttactagtt tgtcctaatg
    11941 gtcttatggt atcaaagagt ttggaaatac aactatataa atacatacca accatagaaa
    12001 tagtaggtat agtatccatt aagcaactaa aagaagtaaa tgtatactac gattacataa
    12061 ttacaactat agatatacaa aatgtaaaca acgtaattgt tgtaaatcct cttttaacat
    12121 catcagatgt tcaattactt atgaacaaac taataagtgt aaaagaaaac gagaagtatt
    12181 tcaatcttga attaataata caagctataa gaaaaaatgg agttatcaat aatgaagaag
    12241 cactaaaagc tgatttatta aacataatac acaagataga tgaaggagag atgtatcaac
    12301 caatgttaaa ggaattaatt aatgcagaaa gggtcaatat aataaaaaat gttagagatt
    12361 ggaaagaagc aataaaaata gcctcaaagc cattgttaga agataattct atagaagagt
    12421 tatatataga aaatatgata aagtcagttg agaaatatgg accatatatt gttttagctg
    12481 atagatttgc tctaccacat gcaagttcta aagaaggagt aaacaagctt gctatgtctt
    12541 tactaatagt agaagatgaa gttgatttat taggaaaacc agtaaatata tttatggtat
    12601 tagctgctgt agataacact actcacatta gagcattagc atcactatca gagatgatgt
    12661 acgaggaaga aaatgtaaaa ctaataatta atggtgataa aagttcaata ctagaactta
    12721 taaataagca aaattaggag gaatgaaaaa tgaagatatt agcagtatgt ggttttggtg
    12781 taggttcatc aatggtatta aaaatgacaa tccaaaaggc tctaaagcag ttaggtatag
    12841 aatgtgaagt agaaaataca gatataaatt cagctagagg tgctgattgt gactgtatat
    12901 tcacaagtca agaattgtta aatgaattaa aaggaacttg taatgtagat gtatatccag
    12961 ttaaaaagta catggattta gcagaagtca cagaagttgt tcaaactttt ttaagtgata
    13021 aaaatgataa gaaatagtat aagttttaaa aagtaaattt aagttaatca gaataaaata
    13081 aaaacttact aagttcaaaa atagtaagaa agagaggagg gatttagtgg tagaaaactt
    13141 aaacagttga cctatcacta aaaaatataa tgcttgattt tatctcaaat aatattttac
    13201 gtaatccatc tatgttaatt ggattaatag cagcatttgg tcttgtacta cagaagaaga
    13261 aagcatctga tgttataaag gggtcactac ttgcagcttt cggaattata attatggaga
    13321 ctggaacagg tatgttagtt agttcaatag caccaattaa tggagctttc caaagtataa
    13381 gtggtggtgc agtaacaaaa ggtttaagtg atgttacttt tactgcaagt tatggtggaa
    13441 ttataggttt agcaatgttt gcgggtttag ctattcacct aatgatagca agatttactc
    13501 ctattaagac aatcttctta acaggtcaca tgctttattg gttcccattc atatttgtag
    13561 cttcaggagt tgaagctgga ttaaatggtg gagtattaat agcttttgca gcagtattat
    13621 cagcactcta ttggtcaata atgccttggt tattaagaaa atatgtgttt gatgttatag
    13681 gagatgattc ttttacatta ggtcatccat ctggttgttt agcacttata tcaggatttg
    13741 ttgctaaatt agtaggaaat aaagaaaaat ctacagaaga tataaacata cctcaaagtt
    13801 tatcattttt tagagaaata tctataagtg gtggaatggc agtgttttta atgtttatag
    13861 ttgttgggat agtaatacca ggtgcgttta aagatagtaa tgagtttatt ggaacgtatg
    13921 caataaagca aggtctttct tttggagcag gtcttataat aatgcttcat ggggtaagaa
    13981 tgctaataaa tcaaataatg ccagcatttc aaggtatatc tgaaaaagtt gtgccaaatg
    14041 cactacctgc ttttgactgt ccaatattat ttaattataa accaaatgct gtattaatag
    14101 gattcttagt tgctatgcta acatctacag ctatgatttt aatatgtaac tactttaata
    14161 tatttaattt gctacttata ccacttgtta taacttcatt ctttgagtgt ggagcagcag
    14221 cagtaatagg agaatcacaa ggtggtatac gtggagctat aataggtaca gctatagctg
    14281 caatatttat ggtagtttta gtaggaatat ctgctgtaat ttactcagga actattcaaa
    14341 attggatact aatatttgga ggtaatgacc tttcattatt tggtataatc agtaggttta
    14401 taggttcact atttggagga atattataga tatgaaattt aaatacatag atgagataag
    14461 aagattaatt gatgtaatag aaaacgaaga aaaagtcaat atggaaaaag cagtacaaat
    14521 ccttgttgaa gcaatagaaa gtaaaaatgc tatattttca tttggagctt cacatgcagg
    14581 aatattaaca gaagaattat tctatagagc aggtggctgt gttcttataa atccagtatt
    14641 tgcaaaatct ctaatgttag atacagcacc tataactcac acaagtaaga tggaaagatt
    14701 agaaggatat ggtacagctc tagctgataa aactccaatt aaaaaaggag atgtagttat
    14761 agttcattca gtatctggta gaaatccagt aagtatagag tttgctatga aagctaagga
    14821 aaataaggca atagtaatat gtataactaa tttaagttac tcaaaaagtg tatcttcaag
    14881 acattcttca ggaaaaaaac tttatgaagt atgcgattta gtgatagata atcatggtca
    14941 aaagggagat gcctgtgtag agatagatgg attaaaccaa aaagttagtc caacttctac
    15001 tgtaatagca actactgtga tgaactctat tatagctcaa gctactcagg aattagttaa
    15061 taaagggcta aagaatcctc cgattttcta tagtgccaat attgatggtg gagatgagtt
    15121 aaataagaag atttttgatg aatataaaag tgtaattcat tatgaatatt aaggttaaaa
    15181 aaatatgtta atgaactttg aagtttatca acaaagaata ttaataatat ataataaatg
    15241 ctatcattta aaagctgtag gtaaaaatct acagcttttt attattgtag attaaaaaga
    15301 tgcgtaatta aattttaaat tggagaaaat aaattggaag gtatcaatta taaatattag
    15361 aaacgttgtc atagaaaaaa actatattaa aaaaaagtac atagaaaaaa ctgattagac
    15421 aataaataca tgctttgata atattttaaa tggagtgaaa atgtgtagaa ttataatgaa
    15481 aaaagaaaat aaaaatgaaa aaagaaaata aaatggaggg aataaaagtg ggtatgttaa
    15541 aaaaattatg ttcactagct atggtagcaa ttatgactac aacacttata acagggtgtt
    15601 caagtggaaa tggaaaagat tcaaaaaagg atggcgaaaa agaaaaacta gttctttgga
    15661 tgccaccagt agaaaataat atgaaggaag tttgggaccc tattttagat aaatttgagg
    15721 aaaaaaataa ctgtgaagta gatttacaga taattccttg ggataattat tctgaaaaat
    15781 ttgcaacagc tataagtgct ggcgaaggcc cagatgtagg atatatgtat gcagaaatgt
    15841 atccacagtt tatagaaagt ggagcagtag aagatttgac taactatgca actaaagagg
    15901 ataaagagca atctatttat ataaaaacat ctgaaatgat gggtggaatg tatggtatgc
    15961 cattccaagc agcaaatcca ggggtattat actataacaa ggatatttta gagaaattag
    16021 gagaaaaacc tcctaaaaca tgggaagatt ttaaaagaat ttgtaaaaaa gcaacacaag
    16081 atactgatgg tgatggaaag atagaccaat ggggattagc tcagggatgg ggagctaaaa
    16141 cttttggtaa cttaaactgg aactggtatc cttatttatg gcaagctggt ggagatatct
    16201 ttaatgatga tttaaaatcg gttaaattta atgataagag tggtttagaa gcagctaatt
    16261 tcttaaaaga attacaagca tatgtgcctg aagattcttt atctaaagat agtaatgaaa
    16321 tgatagaaag tgtatttgga ccagggaaag cagcatttac aataatgtta tcttctgctg
    16381 caacaagtgt atttgatgaa tcattcccgg atttaaattg gggatttgta actggactag
    16441 aagataagaa agcagcaact tttggagcag tagaccattt atctttaatg tcatctgcta
    16501 aaaataaaga acttgcatac aagttaatac aacatatgtt aagtgttgaa tcaatgacag
    16561 aatttcataa agcaatacca agagcaccaa taacaaaagg tgaaccatat caaggtgatg
    16621 aaagattcaa agaaatggtg gaaaatgaca aaaatgtata tagaccatta gtagtaggac
    16681 ctcatggagt tgaaatatat gaatatttat ggaaagaact tcaaactatg atttcaggag
    16741 ataaaactcc aaaacaagcg ttagatgatg ctgcaaaata ttcaaatgat ttattagcac
    16801 aataaaagtt taaagctgta atatgttgta aaggacttta cagattaaaa ttagcattgt
    16861 atttataaat acaatgctaa tatttatatt ggagaggata aaatggaaaa cttagttaga
    16921 gagtttgaag aaaattcaaa aagaaaaaaa acaaaattaa atattaaacc atatatgtat
    16981 atattgccat tgggaataat tttagtggcg ttttatgtga taccaataat aatgtcaata
    17041 tactttagtt ttacaaagta taatattatt agtccagcta cttttatagg tttagaaaat
    17101 tataaaaaat tatttacaga tgaaacatta aaagtatcta ttataaatac tattaaattt
    17161 actgttgttg tagtaccttg ccaaactata ttatcattga ttttggctgt ttggattaca
    17221 ggaaaaggaa atagtaaaat agcttcattt gctaaaggtg ctatatttat accagtatta
    17281 tcatctatgg ttttgatagg tatggtatgg agggctttat tgaatggaga aggttctatt
    17341 atatatcagg tattaggtgc ctttggaata gaatcatcta aacttttagg agatagtaag
    17401 acagcattac ctacacttat gtttatatct atgtggaaaa atatagggta tttcatggta
    17461 atttatattt cagctataat gaatttacca aaacactgct atgaagtagc gaaggttgat
    17521 ggggctacaa aacttcaaga atttataaaa ataactgtac ctttattgaa accaactaca
    17581 ataatggttg tatttttagg gtcgatatgg tcattacaag tatttgactt aatttatact
    17641 gtgacaggtg gaggtccagg tatatctact atgagtatag ttatgcatgc atttaactta
    17701 aactttaaaa actttaattc tggatatgca atgactgtag caaacgtatt atttttgtta
    17761 atagcagtag tttcgatttt acaaaataag cttgtaagac gagacaattc agatttttag
    17821 aaaggtaggt ataatatgaa aacagcaaga agattattaa gtgatggttt acttttattt
    17881 atagcgtgtg caagtttggt accttttata tacatgttga taatatcatt gaagataaca
    17941 tataactcat atagtttgga tatatctttt tcaacagtaa cccttcaaaa ctatatagat
    18001 atttttacta aaaaaggatt tgcacaatat ttttttaata cagcaatagt atctttttca
    18061 ggtgtattat taaatttagt atttagtaca ttagcaggat attcttttgc gaagatggat
    18121 tttaaaggta gtgataagtt atttttattt atgataatga ctttaattat accttcccaa
    18181 gttacaatga taccactata tataataatg aaacacttag gatggataaa tagttatctt
    18241 gcacttatta tgccaatacc aactgcattt ggcgtattta taatgagaca agctatttta
    18301 ggagtaccaa aagagttatt agaatcagca aagatagatg gttgctcgga ttttagaatt
    18361 ttaatacaaa tagtgttgcc tttaattaaa ccagcattaa ttacactagc aatatttact
    18421 tttatgggag catggaacga gtttatgtgg cctcttatag ctacaactaa agatgcaatg
    18481 agaacattaa cagttggatt atctactctt aagacattcc agataactaa ttatggacag
    18541 atgatggcag gggctacaat aacattttta cctccattta ttttctattt gattttacag
    18601 agtaaatttg ttgaaggtgt gtctttgtct ggtataaaag gttaaatagt ctaatttaat
    18661 aatatataaa gaaacttata taaatatgga ggaatttaaa ctatggaaat ttcatatgta
    18721 ctgaaagatt gcacattcaa acacaataaa tactcagtta tagaagctga aacttggaaa
    18781 gataaggatt tggatattgt agtagctaaa ggtgaaaaat ttgcatttca aattatgtta
    18841 aaagcaacag aagaatttaa ttgtacaatt gataaaagta gtgctatatc atggaaaggc
    18901 ctgggaaata gagtaaggtt agcattaaat gtgcctaata gtttagaaaa taattttagt
    18961 ataaatatac taggatatgt tcaagatgat acaaaggcat ttgtgaatga tgctatttta
    19021 agggataaag atgtgttagt agagcaatat ttgcctcaaa cattttggat tgaaggtcaa
    19081 gtaccagaag attttgaaga aaataacctt gatataggta ttgatatatt taaaagcttt
    19141 ggatatgaag atgaagaaaa agtttgtaca attgatgttc ctgtaaaagt aagaaatgtt
    19201 gtgctaaagc ctttagatga aagcaagttc tttttagatt tatggcaaca tccaagctgt
    19261 ttagctagaa tgtataaagt tgagttatgg tctgatatac attttgaaat agtcgataac
    19321 tatttaaaag aattagcatc tttaggagaa aaagttgcaa cagttatagt ttcagattat
    19381 ccatgggcag ggcaaagttg ttataaagtt tataaaaatc catcaaactt atatgaatat
    19441 aacatggtaa gtgtatcaaa aggattggat ggaaagatta aatgtaactt tgagagtatg
    19501 gataggtata taagcatagc tgataagtat aaaatggcta aggagataga tttatttggt
    19561 ctattaggta attggtgtgc tggtgagttt ggaaatcctg tagaaggtta taaggaccct
    19621 ataagagtaa gatattttga tgaaaaggat aaagtattta aatttataaa caatacaaat
    19681 gatttaaaag aatatatagg attagtatta aatcatttaa tagagtgtgg attatgggac
    19741 agagtaagga ttatagctga tgaaccaaat aatccagaag tagtcaaaga gtgtatagaa
    19801 tttataaatt ctactgtagg tactcatcaa gttaaatata aatcagcaac tcacgaccaa
    19861 aactttttag acagagctaa agatgaaatt gatgatatgt caataaattt aaaacttact
    19921 atacaaaatt ataaagatat agaaagtctt aagaaaaaaa tcaatgacaa gggtgggata
    19981 ttgacttggt ttgtatgctg ttttccagag aaaccaaata gttttttaag ttctcctttt
    20041 gtagaaaata gaataatagg atggtatact tattactttg gtcttgatgg atttttaaga
    20101 tgggactata atttatggac agaagaccct tggaaagatt caagttacaa attccctatt
    20161 tggaaagctg gagatatgtt ctttgtttat cctggaaaag atttaaagcc agttagaagt
    20221 gtcagaatgg agaatttaag atttggaata caggactttg agttatttac aatgttagaa
    20281 aaagaaaagg gaagagagta tatagaagtt gagcttatgc aagaattatt aaataaaaaa
    20341 gaaaatgctg aaattaaagg ttttggtgac atagaactag gatattcact tgataattgg
    20401 ggatatgata aagttaaaaa atatgtatta gagttgttag acagagtata aaaaatataa
    20461 gagtgtctca aaatgaactt tttagttcat gaggcactct ttttgtatga aatcaatata
    20521 gtttcattgt ttcaacaatg aaaaatagga ttatttctat tctaagacag ccctttagtt
    20581 ttttagttgt attcaatgtt ttaaaactaa ctataaacgc tataaagttt ataaatgaca
    20641 ataattatat ataacatgat gtactttaat ttgctataat agggattgaa taatattata
    20701 tggaagataa cattgtttgg ggggattaat ataaaaagaa taggtattat tataatgttt
    20761 tttttaagta tatcttttat tggattttat cttgtaataa gagataacag tgatacagtt
    20821 aagatgcaac gagggataaa tataggtaat gcactagaat cacctaagga ttttccgtgg
    20881 gatgtaaaaa tgtcaaataa gttttttgat gatataaaag atgcaggttt tgacacagta
    20941 cgtataccag taaggttttc tgattatacg tcagattctg ataattttaa aattgatgaa
    21001 gaatttttta aaaagattga taaatatgta gattatgcat tggataaaga cttaatagtt
    21061 gtgttggatt tacaccattt tgaagaaatt atgaaagaac caagagttca taaagaaaag
    21121 tttttaaaaa tatggcaaca gatagctaat aggtatcaaa aatatgataa aaaattggta
    21181 tttgaattat taaatgaacc taaagaaaat ttatattcac agttactaaa cgaatacatt
    21241 gaagaagcaa taaagataat tagaaaaact aaccctaaaa gaactattat agtaggacca
    21301 tataatttct atcaaattga ttatttgaat gaattaaata ttcctaaaga ttctaatata
    21361 gtagtttcct ttcactatta cgagccaaat gattttgcat ttcaaggaaa tatctatcat
    21421 aagggatttg agcacttaag caatattact tgggaaggaa caaatgaaca aatggattat
    21481 ttaaaaaaaa ggtttgatac tgtggaaaat tgggcaaata aaaataaggt aaaaatcttc
    21541 ttgggtgaat ttggagtaac aaaagaagca ccagaaactt caaggagagc ttgggtaaaa
    21601 gctgtaagag aagaagctga aaaacgaaac ttttcatggg cttattggga gcttgcttct
    21661 ggatttggta tatacaatca aattgaaggg acttgggata gagaaatatt aagtgcatta
    21721 atagaaaaga ggtgatttta tgaagaaaaa ttcaaaaaag atacttataa ctttaactat
    21781 tattactaat atagtatata tattgtggag aattttttat acagtaccta aagaagaagg
    21841 gatgtttgcc ctaatctgtg ccataatact tctttttgta gagataatgg gtatgatgga
    21901 aatgtttgtg cattattatg gtatgtcaaa tattgagtac cctgaaaaac caatcataag
    21961 tgaagaactt tatccacatg tagatgtatt cattgcaaca tataatgaat ctgttgattt
    22021 ggtgagaaaa actgtaaatg ggtgtattca tatgcagtat ccagataaga aaaaggtaca
    22081 tatatacata tgtgatgatg gaaatcgtga agaaatgtgt attctagcag aaaaaatggg
    22141 tgtaaattac ataactagaa ctgaaagaga aggagcaaaa gctggaaatt taaataatgc
    22201 aatgcaacat acaaattccc ctttgattgc tacatttgat gcagatatga taccaatgca
    22261 tgacttttta attgctacag taccatactt tttgaaaaat gagcaagcta aaaaagatgg
    22321 agaaaaagag gagtatgaaa aggttggttt tgtgcaaact ccacagagtt tttataatcc
    22381 agacttgttt caatttaatc tgcactctga aggaaggatt ccaaatgagc aagattattt
    22441 ttacagagac attcaattgg ctagaaatag gacaaattct gtaatttatg gtggtagtaa
    22501 tacagttatt tcaagggaag cacttgaaga agttgatgga ttttatactt attctattac
    22561 agaagatttt gcaacaggta tcttaattca aagtaagggg tatagatgtt atgctattcc
    22621 agaagtacat gcatcaggtt tatctccgac agatttaaaa agtcttatta agcaacgtga
    22681 aagatgggca cgtggatgta tacaaactgg tagacgtcta aatattttgt ttaggagagg
    22741 tttaggtttt tggcagaaaa taagttatat ttcttcaatt acttattggt atgcaagtat
    22801 aaaacgcttt gtatatataa tggcacctat tttattttct gtgtttaatg tgattgtagt
    22861 aaaatgtacg ttacttcaag tattggtatt ttggttgcca atgtatattt taagtagttt
    22921 atctcttaaa atattttctc aaaatataag aaatactaga tggaccaata tatatgaaac
    22981 tataatgttt cagtctttaa tgccagcagt aatacttgaa acgtttgcaa tatctaaaaa
    23041 taaattttca gtaacaaata agagtaaatt agaagaaaat agaatgtata agtttttaca
    23101 gggaatacct tattttattt acatggtctt atctatcata ggaattttaa aaatgtttgt
    23161 agcaatattt aaaatgagtt caatgacata ttcagttgta ttattttggt tgattggaaa
    23221 cctttttaac cttgttatgg ctacattatt tatatcaggg agacagcagt taagaaagtc
    23281 tgagcgttat attgctgaaa ttgattttaa gttaaagcaa aattcttatg ttctttcttc
    23341 aaaaacaatt gatatatcag aaaatgggtt tgcatttttg ctagaaaatc cagaatatat
    23401 ttccccagaa gaagagtttg aggtagagtt tagagaaaaa tctggaaatg aaatgtatat
    23461 agctaatatg aaagcaaaga ttgttaatgt tgtggaagta aattcaaagt ggaaatatgc
    23521 agcttatatt acacatattg aggatagtga aatagataat tggatgtgta ttgttcacga
    23581 tagaatacct actttaccaa tgacaatatc taatcaacta ggtttctttg atgatttgca
    23641 aattaatgta aaaaaacgta ttgaaaaaac tagaacatta tcacgaagaa gtccaagaat
    23701 caatatgaat tttcagatgg atataaaaaa tacaggtaaa ttaagaatag tgaattttaa
    23761 ttatcaatat gtattattaa attttgagaa taaaagtatt tatcctaaag aaatagcatt
    23821 ggaaattaat gaaaatattg tgttagaatg tgatttgtgt gagggtaaaa ttgatgaaag
    23881 aggtatctta tataaagtaa ataacattga cagcataatg caaaatttct ttttaagaga
    23941 tgaaatgatg gattggatat tacaaaataa gacaattctt gtttcaaagc ctagtgaaaa
    24001 gaaggaaaaa agtatagatg agtttgaacc catggaatat atttaggaga gatgaaatga
    24061 agactttaaa agtgtttttt tgttctacaa aatatcttat tttggtgttt atttttttta
    24121 taaatactac ttttataagt tcagcaacaa gtgataaaat tttagattta tcctttaaaa
    24181 agatagagac tgacttaagt tctaagatta catatgagga tacaggtgta agaatagaaa
    24241 cggatagttc taaaagcgat aaggaacgtt atttatatat ttaccaaaat ataaaagaaa
    24301 attggagcat gtataataat ttttatattg aaattcaaaa taaaaataaa agttctcaga
    24361 gaataaattt aagcatacaa agtaaaaata tgcttgaatt tagattaaaa gaaggtagtg
    24421 aagtattttt agaaggtaaa aatataatat attcagataa aataaaagaa ggatgcatag
    24481 aagtaccagg tgaatttgaa ggcaaaattt atgtgaactt taatagcctt ataaatgagg
    24541 aaagtaatgt tgtgctggac tcaaatatgc tttcaaacat tgttagttgg ggaattacgt
    24601 ttattccatc tgatgaagaa cataatattg ttattataaa aaagatatct ttattgtctg
    24661 aagagaaatt aagattttta aataatatta aaataattgg cgatgaagaa gtacaaatac
    24721 cagtattggg tcaaagtata agtcagtatg aagtattagg tcttaaaagt gattctaaaa
    24781 ttaagtactc tcttatggga aaacaagata atgtgagcat atctcaaaaa ggtaaattaa
    24841 cattaagtaa taaagccaaa cctggacaaa ttattttaca agttaatgtt gatgataagt
    24901 ttaaaattgg aaagaaaata acattaactg agtcttggag tgttaataaa aaagataagg
    24961 atggtgttcc atatacatta gtatatcctg agcaatcacc aacagtacaa gatatgaaaa
    25021 aaataaactt tatgaataac atcataacgt ttgttcgaat tttatttgta tctctagtaa
    25081 taatatgctt tggaatatat ttatattgga aaaaatgttc aaaaactaag taaagagagg
    25141 aggttagatt gtgttatttt caagtttaat ttttttattt tattttttac ctataacttt
    25201 ggttttatat tatgtattta ggtttaaccg aacgatacag aacatgatat tgcttgctgt
    25261 tagtttattt ttttatgctt ggggagagcc aaaatttgtt gttatcatga tagtttccat
    25321 aataatgaac tatatatttg gtttactggt agatagatat agggaaagta aaataaaagt
    25381 aaaagttttt ttagttttga tgtgtgcata caatataggt gttttattta tatttaaata
    25441 tctagctttt gcgctaagaa atattagtga acttattagt actgaattga ctatacctag
    25501 tattgtttta cccataggaa tttcattttt tacatttcag ggaatgtctt atgttataga
    25561 tgtatatcgt agacatggag aagtccaaaa gaaccctttt tatgtgggat tgtatatagc
    25621 atttttccca caattaatag caggtcctat agttagatat gagagtgtgg cagaacaaat
    25681 tctcaataga aaagaaactt ggaataaatt ttctattggt acttgtaggt ttataacagg
    25741 tctaggtaaa aaagtattaa tttcaaataa catggctata gtagcagact atatttacac
    25801 tatgaatagt caaggagaaa tagctgcatc acttgcatgg cttggttcaa tagcctatac
    25861 tctgcaaata ttctttgatt tttctgctta ttcagatatg gctataggct taggtcttat
    25921 gtttggtttt aaatttgaag agaactttaa ttatccatat atatcaaaat caattagtga
    25981 gttttggaga agatggcaca tttctttggg aatgtggttt aagagctata tatactttcc
    26041 gcttggaggt tctagagtag caaataaaga tattatgatt agaaatatgt ttatagtgtg
    26101 gttattcact ggaatatggc atggagcaga atggacattt gtaatttggg ggattttaaa
    26161 ctttgttttc ctaattatag agcgatttat aatgtttgaa aagattgaaa atcacaattt
    26221 tataaaacat atttatgctt tattagtagt taattttgga tgggtattgt ttaggtcacc
    26281 taatttaaaa gaagcatata attattttaa agctatgttt ggaggcaatg gagctatatg
    26341 gagtgattat acatatatgt ttttaaaaga atactgggtt ttctttatat ttgcatttat
    26401 ttttagtgta cctattgcaa agaaaatcaa taagtttgtt gttgaacaag ctaaatatag
    26461 tatgatattt aacgtatttt atccaatctc agctatttta ttgtttttta tttctgtgac
    26521 atatttagta actggaagct ataatccatt tatttatttt aatttttagt tgggaggaaa
    26581 agtatgaata gattttttaa agtaagatta ataactgcaa ttatgttctt aattatttta
    26641 ttcgctttct ctatctttaa tgtttgttat tctggaaaag atacgataca ggaaataaaa
    26701 aattcaatag ctgaaaaaag tagtttaaaa gatacaatat catctattga taatacagtg
    26761 aatgacaaag taatatacaa aaatacgttc attgaaactt atggctattt acaaaaatta
    26821 atgggtaaaa atgagttttc aaaatttagt gttgtaaaag atacttctgg aaagatgcat
    26881 tatacatatt ttgctaaagc tccaaatcca gttggtgtga tagcagatag ggtaagaaaa
    26941 ttttctgatg aaatggaaaa gcaaaacact aaattggttt acctaatgac tccagataag
    27001 tatataaaag gagtgacaaa atttcccaaa ggaatacctt attcttatag taatgagaca
    27061 gcagataatt ttttaaatca actaaaaaag tataatgtgg actatattga ttttagagaa
    27121 aatattttaa agagtggtat tccaaaagat gatttatttt ttaaaacaga ccatcactgg
    27181 aaagtagaaa cttctttttg ggcatttgga gaattagttg agcaacttaa taaaaaatac
    27241 aatatgaatt tagatgaaaa tcattattat agagataagg acaattataa ttcaattgta
    27301 tatcctaagt catttcttgg ttctatggga agaaagactg gtatcttata tggtggtata
    27361 gatgatttta cattaattta tcctaaattt aagactaatt atacttatta tacagattct
    27421 aaatctcaaa aatttgaatt aaaaggaagg tttgaagaga gtttaatatt atcatatcct
    27481 tttaatgcag accttaattt aatggatggt caatcagata agtattttac atatttattg
    27541 gggaacagac cattagttaa aataaaaaat atagaaaatc cagatggatt aaaagtgctg
    27601 tttgttaaag attctctaat tgtaccaaca gcagcatttt tttcctctgt ttgttcatct
    27661 atagatatga tagaccctag atattatgat ggagatatac tagaatatgc aaaatcccat
    27721 gactatgact ttgtttttat atctgtatat ccccaaaatc ttacaaaaga attttttccg
    27781 ttttgtgagt aaatttctaa aaaagttttc tgataaaata aatggctaac tggtaaaaag
    27841 ttagccattt attttgagtg ttattctaag aaatattttt tttactaaat acttctttat
    27901 ctatttttcc aagtgatttt cctacagtaa ttgatgatac catagcacca ttaacattta
    27961 tcatagtacg tcccatatct agtatagggt ctattgcaag tataccacca actaatggaa
    28021 aataagaacc aagaccaact ccagagataa caactgaaac ggccattgta gcagttcctg
    28081 gaagaccagc tattccaaac gaactaattg ttataacaat taacaacata acataaaagc
    28141 tcatatccat gtttactcca gccatattgg caagtgttac agccattaaa gcaggatata
    28201 tacctgcaca accattcata cccatatttg cacctaaact tgctgtaaag cttgatacac
    28261 cctcatcaac tccgtgattt tgtactagac tttctatcgt tacaggcaat gttcctaaac
    28321 tagaacgtga agtaaatgcc agaagtaaag cttcagttga attttttata taagtaatgg
    28381 gatttagacc atttaaagtt ataaaaatta aatgtatgat aaataatatt gcaactgata
    28441 tataaagagc taatataaac tgtaaaacag atactattga actaatacct tgtgaagtta
    28501 ttgtatttgc aagcagagca accacagcat aaggcataaa tttaataaca gtcatagaca
    28561 cacttacaat tattttgtaa gatgcttcta taaaatctat aaatggtttt attgtctcaa
    28621 aatgtttttt acttaatctc tttattgagg tagctataaa tgtagcaaat attattgtag
    28681 ctacaatatt tccatttgcc atagccatta caggattaga tggtagtaat cctcttaaag
    28741 tatccacaag agagtttatt tctttaagtt cttgattttc aactaaagta ggcatattag
    28801 ctcctacacc taacttcatt atatttgcaa cgataattcc aacaattgca gctatagcag
    28861 tagtagcaag aagcatacct aaagatttaa atgtcagagt tccaagattg ttatctttca
    28921 tatttataat tacttttata atggataaga aaactaaagg tactactaac atttttaata
    28981 agtccataaa tccagaacca aataggctat accacttact tacttcttgt agccaaatga
    29041 cttgagttgt atcattaggg aaaccagcaa aagcttgtat tatgattccc aatatgagtc
    29101 ctagtactgt agcatatatt gttctcaatg aaaattttat ttttttcttt tcaaattgtc
    29161 taacaataaa gaacgtacct agaagtaata ttatgaaaaa aattgtttta aaatctgtaa
    29221 tcataagaaa ttgtgataaa aatgaacttt ccaatatttt tctcctttta tttaaaaata
    29281 aatttttcta agtttaaatg atatcaatat attaaaaaaa tgtcaatatt atatcacaat
    29341 ttacttaaat agagtaaaac aagttgattt tgaaaaatgt gattaagtac agaaaaatag
    29401 ggtataataa atgtttaaag aatttgaatt gtggtaagaa aataaaaaat agcttttata
    29461 agatttgatt tcaaattaaa atgtgttaaa taaataacta aaaattatta taaaaaatgt
    29521 tttcatagaa tatatatcaa atgaaaagct taaaataagt gaagaaatta actagacatc
    29581 aaaggagaga taaaatgaga gtaataataa ttggtggtgt tgcagctggt atgagtgctg
    29641 cagcaaaatt aaaaagaata aaacctgagt atgaagttgt tgtttatgaa aaaactgaaa
    29701 tagtatcttt tggtgcttgt ggattaccat actttgttgg tggatttttt aatgatgctg
    29761 atgaattatt agcaagaaca ccagagaaac ttagagaagc tggaattgat ttaaatatat
    29821 ttagagaagt cgttgaggtt gatagtgaat ctaagaaaat aaaagtaaaa aacataaaaa
    29881 cagatgaaat atatgaagat tactatgata aattgatgat agcaactgga gcaagaagta
    29941 ttatgccttc tataaaaaac ataaaattga agaatgtttc tacattaaaa agtttatatg
    30001 atggagaata tctaaaaaaa cttttaagta atgaagataa taaaagagtg acaataatag
    30061 gagctggttt tatagggtta gaagcagttg aggcatgtaa gaaattaggt aaagatgtag
    30121 atgtaattca gttagaagat agaatattgc cacaagtatt tgataaggag atgacggatg
    30181 tattagaaga agaaataaat agacataatg ttaatttgca cttagatgaa atggttgtag
    30241 aactttgtgg agaagataaa gtagaaaaag ttataacaaa caaaggggaa atagatacag
    30301 atgtagtaat aattgctact ggcgtgagac ctaataccga gtttttgtca aatactaata
    30361 taaaaatgct aaaaaatgga gctatagtag ttgatgagta tggaagaact tctgtagaag
    30421 atatatactc tgctggagat tgtgcaacta taaagaatat agtaagtaat gaaaatgtat
    30481 atgttccttt agctactgga gctaataaat tgggtaggat agtaggagaa aacctagcag
    30541 gaagagaagt atcttatcaa ggttcactat cttcaagctg tataaaaatt atggatatgg
    30601 aggcagcatc aactggaatt acagaaaggc aagctaaaga tttaggcata aatgtcaaat
    30661 ctaaatttat ttctgattat aatcaaactc attattatcc aggaagaaat aaaatatatg
    30721 taaagcttat ttatgatgca gatacaaaag ttattttagg cggacaagtg gctgggttta
    30781 aggatgctgt ccaaagagca aatgtattgg cagctgctat ctttggaaaa atgaccacaa
    30841 gtcaacttgg aatgttagat ttatgttatg ctccaccatt tgcaagaaca tgggatgttt
    30901 taaatgtagc tggaaatata tctaaataat atttagatta tagctaatat agttgacaag
    30961 caattaaggg aatgaattta cctaaactat ttttatcatg gtaaaattca ttcctaaatt
    31021 tttattccaa atttttattc caaattttta gaaataagga ttccattaaa ttattcatca
    31081 aaatttaaac gcaagcttaa tctatcttct ccaagaatag tattttcaaa aacattagta
    31141 gcattttcta aatacatact aggtatgtct aaagaaggac taaaatgagt cagtaaaagt
    31201 tgtttgacat ttccaagttt tgcaagatta gcagcttctc taaaagtcat atgtttgttt
    31261 ctaacagctt ttgatatatc taaatcatca ccatacatgg cttcacatat aaataaatca
    31321 ctatctttta tgaaatctgg tattgattct ataggtcttg tatcggttgt aagacttatc
    31381 ttaactccct ttcttaattc tcctaagacc atatctggag tgtattgttt tgaatctaaa
    31441 actatatttt gaccttcttg gagtttgttc cataatattt taggaacttc gtttttagtt
    31501 gccttatcta tatcaaattt agggtttctt ctaaaattaa aactgtatcc taaacactct
    31561 atagagtgtt ctaaactgat tgttgaaatt tctaaatttc ttaaaacttt attatctaaa
    31621 gaaaaattgc cttgagggtt ttctataatt tttaaagtgt atggtacata ttctactaaa
    31681 tttctcatag aacgtataca atcgactatt ccaactggac caactatagt taaatcactt
    31741 gtacgaccac tattcccaat tgttgataaa agtcctaaaa gtccaaatat atggtcacca
    31801 tgtaaatggg ttatacaaat caagtctata tctttaaagc cacatttttt tagcttcata
    31861 gatacctgag taccttcacc acagtcaatt agtatttttc tacctttata gtttataaat
    31921 acagaggata gaaatctgtt tggcataggt acatttccac cacatccaag taaagttaaa
    31981 tctatcattt tttccccttt ctaaaattaa cacaaatctg ttgtttgttt ttattatgat
    32041 atcctattat aattatttta acattaaata taacaataaa tgtgtatttg agtcaagaaa
    32101 tattgtcaaa taaagttttt atataacaat gtataaaaat ttggaggaca aaaagtgaaa
    32161 attatattta gaagtattta aaggaggaaa atttaatgaa agtattaaag aaaattacta
    32221 ttttgttatt gattacatta actttagttg gttgtagtaa cggagaagag aaaaaagtgg
    32281 ttgaaggttc taagaaaact caaaattcta aaaaagatga acagcctata atagtgcata
    32341 aagatgcaaa tatattgaaa gataggtatg agccaccaaa aggatttaaa agagttgatg
    32401 tagaaaaagg tagttttgaa tcatttctta ggaatactaa gctaaaaaat tatggagaga
    32461 aagtaaagta ttatgatgga agaactaagg aatcaaaagg tatttatgat agtgtatttg
    32521 atgttgatat tggaaataga gatttacatc aatgtgcaga tgcaattatg cttatgagag
    32581 cagaatactt gtatcagaat aaactatatg atgaaataag ttttgatttt gttgatggtt
    32641 ttaaggcgaa gtattcaaaa tgggcacaag gatatagaat ttcagtaaaa gatagtggtt
    32701 caagctggta taaagcaact gaatattcaa catcttatga gagtttcaga aagtttatgg
    32761 atatagtgtt tgcatattca ggaacgttat ctcttgaaaa agaattggag cctgtaaaag
    32821 ttgaggatat gaaaatagga gatgtgttta tagttggtgg aagtccagga catgcaagta
    32881 taattatgga tatggcagaa aatgaaaaga ctggtgaaaa ggtatttatg atagcccaaa
    32941 gctatatgcc tgcacaacaa acgcaacttc ttattaatcg taaaaatcca aatttgagtc
    33001 cttggtatag tttagatttt gaaggtgatt tgacaatacc tcaatggact tttaaaagag
    33061 accagttgaa aagattttaa aataataatt ttatataata aaagaaaaat atagatgtga
    33121 aaaaatgtaa ttagattaaa actaattgga tatggacaaa attaaattga tagataaata
    33181 ttaaaaatta cactaattaa tcttactata gtttaatatt tatctaaaaa gttttatgta
    33241 aaatataaat tttatattaa agctaaaact aatttgagga ggctaattta tattgaagat
    33301 tagaaggtat ttaattttgg taattataat tctaattaca acaagtcaga gttttatttc
    33361 ttatgggcta gagcgatttg aagaaataac catttttcac actaatgata tacatggaag
    33421 atatgctgaa ggtgatgacc atatacaaat aggaaatcta gccacattga aaaaagaaac
    33481 tccaaactca attttagtag atgcaggaga ttgtttgcat ggacttccaa tagtaaatat
    33541 ggatagaggg aaaagtgcaa ttgaattaat taaatgtgct ggatatgact atattactcc
    33601 tggaaatcat gactttaatt atggaaaaga tagactgttt gagttatcta aaagtgctaa
    33661 tttaggagag aatactttaa aatttttatc atcaaatatt tttgaaaatg gaaaaaaagt
    33721 ttttgatagt aatgatataa aagaaataga tggaataaaa ataggatttt ttggactctc
    33781 aacccaggaa acaaagagca aaactggatt taaaaatgtg gaaggattag agtttaaaaa
    33841 tcctatagaa agtgcaacag aacaagtaaa tgaattaaaa ggaaagggtg ccaatgtaat
    33901 agttgcaata tgtcatattg gtacaaatag tttaagtaag ccaaatagta tagatatagc
    33961 aaatgaggtt caaggaattg atttgattat tgatggacac agccacacta agtttgaaaa
    34021 tggtaaacaa gtgggtgata cattgattgt aagtgcagga caatatttag aaaatattgg
    34081 cgagataaaa ctaactttgg atactaatga tgtagaaaat ataaaaatag aaaataaatc
    34141 agttagatta attggaaaag aagaagcatt aaagtataag cttgactccg ctgtaagtaa
    34201 aaaggttgat gaaataaaag aacaacaaga aaaaatatta gataaagtta taggggtaac
    34261 tacaaatact ctagatggtt catatgaaaa tgtaaggaca aaagaaacta atttgggtaa
    34321 tttaataagt gacattttac tagataaaac taaggctgat atttctttat ttaatggagg
    34381 gaatattcga gatacgatag aaaaaggcaa tataacaaga agagatattg tagatgtatt
    34441 tccttttagc aatactatag ttaccaaaga acttacagga gcacagatta aagatgtatt
    34501 ggagcatgga gtaaaacttt atccagaaaa aagttcagcc tttttgcaag tgggaggaat
    34561 ttcatattac tttaatccta aacaaaaaga aggcgaaaaa ataacaaata ttcaaaaaga
    34621 aggaaagcca ttagatttaa ataaaaagta tgtagttgca acgaatgatt atatagcatc
    34681 aggtggagat gaatttcctt gtttttcaaa agagccagta ctaaaggaat ttggaaattt
    34741 agagagtgca gtcattgaat atatagagta caaaaaagaa atatcaaaaa atgtagatgg
    34801 aagaattggt attaaatcta aagatgagtt aataagcaag aatcatgaga gtgatttgat
    34861 attaagtgaa aatgaaaaaa atatcaatga taatgaagag aatctatcta ataatataaa
    34921 aagctctgat agtgataaaa ataaaagctt agataaaaca agtgctagag aaagttctaa
    34981 tctaggaata tctaaaagag aagctaaaga aaaatctcct aaaacaggag atttaggttt
    35041 ttcaaatagt ataattatat ttatagtttc aagtacattg atatgtttat tgaattttaa
    35101 tcaaaaagag ttaaaggata aaaagtcaaa ataaaaatta atgagcaaaa attagtagat
    35161 agtaatttaa ttttagataa tagtaaacag caaaggaaat tagttatata aatactaatt
    35221 tcctttgttt tatgttttaa catgattact tttaaataaa attgtatttt atgaaaaata
    35281 attacactaa atggcatagt attatgggga tttttagttt ttattcaagt aaatgaaatc
    35341 atattaacaa cttctgatat atttacaaat tgataaaaaa atgattttat tagtatgata
    35401 taatttagct agtatatatg atgatggagg atatagggat gaataatata ttacttttgg
    35461 aagatgacaa atctttaaat agaggaatta gttttaaatt aaaaaaagaa ggatacaatg
    35521 tttttagtgc atttggtata gaagaagcaa aaaatatttt cgcaaaagaa gagatatgct
    35581 taattatttc cgatataggt ctacctgatg gtagtggttt tgacttttgt gaggagatta
    35641 gaaagaaaag tgatgtttac ataataatgt tgacagctct tgatgaagag gtggatattg
    35701 tcacaggata tgatttaggt gcagatgatt atatcacaaa gccttttagc ttgatggtat
    35761 taatatcaaa agtgaatgcg cttatgaaaa gagtaaatac agtaaaaaat tatactctat
    35821 tagtttgtga tgatttattc ttttattata tagaaaataa attaattata agaacagatg
    35881 ataaggaaga agagataatt ttaagtaaga cagagacaaa actattaaaa tatttgatgg
    35941 aaaattctat gcagacattg actaaagagc aactgttaga atctttatgg gatagtagtg
    36001 gtaattttgt agatgataat acgattgcag taaatataag aagacttaga caaaaggttg
    36061 aaaaaaatcc atctgaacct aaatatataa aaacagtaag aggggttgga tatatgtggg
    36121 gagaaaggag tataaaaaaa tgctaaaaaa tatgaacctc aaagatggtt taagtgtgaa
    36181 agatgattat ataatgagaa gagttattgt atattctgtt ttatctgtac taatagtaac
    36241 ccttgtattt atactttttg aatatagcag attacataaa ttgtatgaac atcaaataaa
    36301 aaatactcaa aatattgctg gaatattgat tgaaaaatat cctaatgatg aaatagatat
    36361 agtaaattct atttataatt ctgattatag taaggtgtct actggagaaa aggcatttaa
    36421 gaaatttggc tatggattgg aaaataaaat gtcaaatgat aaaaatttta gcttatactt
    36481 aaaatctttt tttaaggaat cattttttat ttttttaatg atgtttatag tagttatagg
    36541 tataatatat tattttataa gatacataaa taaaagattg agtaaaattt attttatagt
    36601 agataagatg tcacaagaaa actacttgaa ggacactact gagtcagaag aaactggtta
    36661 tctagtagaa tataatgaat actttaaaga aggcatattt agtaagataa ataactgtct
    36721 atatgagctt aatagaagtt taaagataaa gtttattaaa ttagaaaaag aaaaagaaag
    36781 tgtaaaatca ttagtaacag atatttccca tcaattaaaa actcctttag catccttaaa
    36841 attatacaat acattactta tagaagaaga attagaagaa gatgaagcca acgagttctt
    36901 attaaccaat aaggcttcta taaataaatt agaaaattta ataaattctt tagtgaacat
    36961 atcaaggtta gaaattagca tgataaacat aaaaaaagaa gataatgata taaaaaatac
    37021 tattctaaat gcagtaaaaa gtgttacccc taaggctaag agcaaaagta taacattaaa
    37081 tttaaatgaa tttaacagta gaattatacc acatgataaa aagtggactg aggagagtat
    37141 ttttaatgta ttagacaatg ctgtaaagta tacacaggta aatggagaaa taaatatatc
    37201 tgtagaagaa actgctaatt tctttaaaat tataatagag gataatggca tagggattga
    37261 aaaaaatgag tttaataata tatttaaacg tttttataga ggttctgctg atgaaattga
    37321 atctgtagaa ggtagtgggg taggattgta tttaagtaga aagattttag aagaacaagg
    37381 tggaaatatt atagtttctt caaaagtagg aatagggagc aagttttctt tgtttcttac
    37441 aactatgtaa tattaaatga aaggaaaatg taagcctcta catttattat ataagtataa
    37501 acagctccag aaataaaaaa ttaataaaat atggagtaat ataacttaga atgagaggat
    37561 aattttatga aaatactata tactgaaaat ttatcaaagc attatggaaa aggagaaagc
    37621 ttagtaagag ctttggacaa tgttgactta gaaataaatg aaggtgaatt tgttgcaata
    37681 ataggaaaat caggttctgg aaaaagtaca cttttgcata tgattggagg tcttgatata
    37741 cctacatctg ggaaggtgta tatagataat aaaaatatat ttacattaaa agaagaagaa
    37801 cttgctgtat ttagaagaag aaaaataggg tttatatttc aaagctataa tttaattcca
    37861 tctcttaatg tatgggaaaa tgtagtgttg ccaatcggtt tagatggtag agaagtagat
    37921 gaatctttta taaaagaact tttaaaatca ttaggacttg aaaataaaca cgacgtttta
    37981 ccaaatacat tatcaggtgg tcaacaacaa agggtggcaa tagcaagagc attagctaca
    38041 agaccagcta taatacttgc tgatgaacca actggaaacc tagattctaa aacatctgat
    38101 gaggttatga gtatattaaa atctatgtct aagaagtaca gtcaaactct agttatgata
    38161 actcatgatg acagtatagc ccaaatggct gatagagtta tattcataga agatggcagg
    38221 gtgtcaaagg ttggtgataa aaatgattaa tactactaaa atagcaaaaa gtaatttaaa
    38281 gcaaaataaa tcaaaaagta ttctaataat aattactata atactttcaa ccttaatgct
    38341 ttcttctatt ggaatttata ttgttgatgc aggagcatat caaaaagaaa acactataaa
    38401 gtattcaggt aattatcaag gtatattagc aaatgtagat gaaaaacagg ctgatatatt
    38461 gagtaatcat gcagatgttg aattaactgg tgaaatgaat ggagtaggtg ttgaaaaact
    38521 ggaagatgat tcaaatattt ctctagcata catgaatgaa gatgctttga aattaaatag
    38581 ttttgaattt atcaaaggaa aattgccatc aaaagagaat gaaattgtgt tagatagtgg
    38641 agctttaaag gcacttggtt ataaaaataa agatttaggt gaaaagataa aaatatctta
    38701 tgatgactat aaaaatgata aaaaaattga aaaagagttc attataagtg gaatcttaaa
    38761 aactagcgaa atatctgaag caggtaaata ttattatgct attatatcag aatcatatat
    38821 gagaaatact agagatatgt cacaagaaga ttttaatatt tatgtgaaat ttaatgataa
    38881 aagtaattta tcaatagagc aagcaaaaga aaaattagat aaaattgcta atgatatagg
    38941 actggatact attaatactg ctgtaaatga aaattatata aatgctttaa aaccagatat
    39001 ggaaacgatt atgggtggag tttttgtagg gttagtaatt gtattatcta gtattttagt
    39061 aatatacaat atattttata tttcaatagt tacaaaggtt caagaatttg gaaaacttag
    39121 agcaattggt gcaactaaga aacaaataaa aaatatagtg tttaaagaag ggttcatatt
    39181 agccggaata gcaataccaa taggaataat tttaggctat gtacttgcaa atattataat
    39241 aaaatcattt atggatatag atgtaaaatc atcacgatta ccagttatat tatcggttgt
    39301 ggtaataagc tttatatctg ttgttttatc acttttaaaa ccaatgaaag tagcatcaaa
    39361 agtatccatt gttgacgcag taagatatac aggaaataaa ataagtaata agagtaagag
    39421 aaaaggatat aaaaatataa atctaaagag attatcacat gccaatttag aaagaaataa
    39481 aaaacgtact tacatgacat tggcatcact tatacttagt ggaactatat ttataacagt
    39541 atcaactgcc ttagaaagtt ttgatgctga gaaaatggca agagaacact ttccttatga
    39601 tatagaagtt agactatctg gttatgaaat gaacagtgat aaaaatccta aagataattt
    39661 gaatatttta caaatggata atcctctaag taaagatttt tttaatcaaa taaagaacat
    39721 ggaaggaatt aaaagaatag aatctgcaag aagtgtaaaa attggtatgg aggactatga
    39781 tgttgaattt aagtatgacc ttttacaaag tataaatgaa aatgatgtaa aatcattaaa
    39841 caagaatata atagatggaa aaataaatct tgaaagactt caaactggag atgaaatagt
    39901 aattactcat gttgatacag caaaagaaat gggagtaaaa gcaggagata aaataagatt
    39961 aactttatat gatggagata aaaagataaa aaaagaattt aaagttcaag caatagctat
    40021 gggggttcca agttttggta tagggaaaga ttttatagat agaactttga aatacgattc
    40081 aactagtagc ttgggaatat atacagaaga aggaaagtat caagaaatca aagatagtat
    40141 caagaagatt gctaaaaata atggattttt agagactgac tttatagata gtagaattga
    40201 gtcaaataag gctactattt catttataaa gataatggga tatacgctaa caggcataat
    40261 aggtgtgatt ggatttatga atcttgtcaa cacaatgata actagcatag ttactagaaa
    40321 aaaagaactt ggtatgcttc aagctattgg tcttactaat aaacaattag tgaaaatgct
    40381 taattcagag gcaatgtatt acacttctgg aatgatgata ggaagtattc tatttggagg
    40441 tatattaggg tatattgctg ttatatttct aaaaaagact ggattatctt atgcaacata
    40501 ttctttgcca atagtaccaa tcttgttgat gattgtgtgt attcttatag ctcaatttat
    40561 aacaacttat ttgataggac gaagttttaa taaagaatct cttattgata gagtaagata
    40621 cagtgaataa aatgcaaata tttatggtta gtttaactag caaaaaacat ataaaaaaat
    40681 atagttaaag aaattcataa ttaaataggt taaagcatag tacagagcaa acaatttaaa
    40741 cagatgtttg ctctgtattt tattatgtaa tcaataatat atctaatata ctgatattat
    40801 tcatgttaga aaatgtaccg gtacaattct aaaaaagtat aaaattgtac catataaaat
    40861 gaaaattttg tataataatc gtatagaaaa taaataggag ctgatatata tgaataagaa
    40921 gattaattat gcaccaggac caactgaaac tagggaaaat gtaagattag caagagcaga
    40981 aaaaacaaca aatccagata tagatattga ttttgttgaa ttttataaaa agacatgcga
    41041 gaagtttggt aatataattg gaacaaaaaa cgatgtttac atattgagtg gtgaaggaat
    41101 attaggtctt gaagcagcat gtgcatcact tacagaaaaa ggagatagag tacttgtaat
    41161 tgataatggt atatatggag aaggttttaa agattttgta acaatgtatg gtggggagta
    41221 tgtgcttttt tcaagtgaat atactaaaag tatagatatt gatgaattga aagcattttt
    41281 agatgaggat agtaatttta aatatgcaac agtagtgcat tgtgatactc caactggcgt
    41341 attaaatgat gtaagcaaaa tatgtccatt acttaaaaaa tatggaatat taactgtagt
    41401 tgattctgta gcaggtatgg taggagaaag attaagtgta gatgaatcta aaatagatat
    41461 aatattaggt ggttcacaaa aagctatttc tgcaccagca ggacttacta tagtcggaat
    41521 aagtcaagat gcaaaaaatt gtataaaaaa tagaaaaact gatgtaatag gtttttattg
    41581 taatttaaat atatgggaag gctattatga aaaaaaatac ttcccttata caatgccaat
    41641 aagtgacata atggggcttg ataaagcact tgataatata ttagaagaag gaatagaaaa
    41701 tgtattaaat agacatgaaa agatagctta tagtgttaga aaatctattg aggaatatgg
    41761 tctagaacta tttttagaag aaggatattc taatacagtc acagctataa aagttcctga
    41821 aagtatagga gctttagaac ttactgatta tatgcttaaa aattataata cacttgttgc
    41881 aacatcacta aatcaatata tggatactat tttaagaata ggtcatatgg gagagaatgc
    41941 aaatctaaat aaaatagaac atgtactaaa tgttttggat aaaagtttaa gtgcactagg
    42001 attcaaagga aatggaaatt tgttaaattt atttaataag tattattttt aatagatagg
    42061 gggatatagt atggaagtta cagctaagca aaaatttaca acgaaagaca tggtggaaac
    42121 ctcactatta atagcattag tatttattgc aactaaattt ataaacataa gattaccaat
    42181 atctataaat ggtgggcttg tgcatttagg aactgcaatg ttgttcatat cagcaatagt
    42241 atttggaggt aaaaaaggtg ctttatcagg ggctattggt atgtcacttt ttgatttgat
    42301 atctgggtgg actttatggg caccgtttac ctttatagtt agaggagtta tgggttattt
    42361 acttggaaag attgcatggg caaatggtaa taatgggaat aattttttaa tcaatgtaat
    42421 aggaatatgt gtctcatcaa tttggatgct atttggatat tatgtaacag aagttatact
    42481 ttatggtaat tttatagtac ctcttacttc aataccaggt aatttaatgc aagtattgat
    42541 aggtcttata atagcgcttc ctatttctaa ggtattgaaa aactgtataa aataaatata
    42601 aagtgaacta aaatttatga tagcataaat tatactgtat aatattagga tggagtacat
    42661 aattaaaaaa ttttagtata gaggttagtt cacaatgtta tcagttacaa gatattcaat
    42721 caaaaatgtt cgtttaaagc cgtgggtaaa gtttatttgg tattttgaga caaaatcaaa
    42781 tatactgtta aataataagt tacttccgac agacagtata gatattattc tcaatctttc
    42841 tgatgttatg gaatataaaa ttgaaaatca agactatact gctagtaata tgcattttaa
    42901 tggaataagg gataaacatg gatttattat tcaacatgga aatatacgtg ttatagggat
    42961 ttctttttat ccatttggtt tatatccatt tttaaagata ccaatttcag agtttaacag
    43021 acaaattgtt gacttagaag tagtatcaca gctatttgca aaaaaattgg aagagtcatt
    43081 aagtccaaca caatcagtag aaaaaattgt tttatattta gaagaagtat tattatccat
    43141 attagaagaa gatttaattt caaataaata tgtaaagctt ctaaattcat ttatatatgt
    43201 taatagatat agcaatataa aggcattttg tgatgataca aatataaata ttaaaacatt
    43261 ggaaagaatc tgtttaaaat atacagggta tacacctaag attttaaaga gaatttacag
    43321 atttaaaatg gcaagtaatc aacttattta taattataaa gatgatgaac tttttgattt
    43381 tatttatgaa aatgagtact atgaccaagc acactttata aaagaattta aaaaattttc
    43441 agggacgtca ccaattaaat ttattggtga gaataagacg attaaagaaa atataaagta
    43501 cagctattta taattatact gtcgattttt tacaatacct ccaatttatt ttttgatatg
    43561 atataggaaa ataaattgga ggttttacat atgaaaatta ttacagaaat agttgagttt
    43621 aatgtggaag aaaatttatc aaaagaagag tttatagaaa ttgtaaatga cttagaatta
    43681 aaatttcatt ctattcaatc tggttttatt gatactgagc ttttatatga tgaaaagtct
    43741 caaaagtgga ttatgataca acattgggaa tctatagaag acctcaagag tgcttctagt
    43801 aaaatgttta aagattcttc aactgagaaa tttagaaaag ctttaaagaa tcaaactgtt
    43861 aagatgaata taataccaca aattaaaagt tggaaattat agaacttaat gtaaaagata
    43921 gttaaatagt atcaaactaa attagccaaa taacattttg tatttaaatt gttatttggc
    43981 ttttagtgtt tattttaata tgagtgttat atactagaaa aaccaacctt aaatactact
    44041 atctaaaagc actattgctg tacgagaggc aataggcatt ttagaagaag gaggagttga
    44101 gctaaagtat acatcataat ctcttaagtt ttcttttttt gtgtttcctc caaactctac
    44161 catatcagta tttaatatcg ttttgtagtt tgtattttga aactttgaaa tatcaactga
    44221 ttgtattaga ttagggtgga aattaaatat aaataaatat ttgcccttac tgtacaccaa
    44281 aatcttttta tcattatcca tatgtataag ttcatgacta ttttggataa aaatatcact
    44341 gtattttgta agttcgagca tagatttatc aaaatttaat aattgtttat actttaagtt
    44401 gttatcttct gaaaggctcc attgtcttct ggcatactta tagctccaat tgttgccttc
    44461 tcgtggaaaa tcaatccact caggatgtcc aaattcattt cccataaaat ttagatatcc
    44521 ttcacctgct agagaaaaag ttattaattt aattaattta ataagagata ttgctctatt
    44581 gataacatgg ttatttgagt ttatttccat attccagtac atttccttgt cagctagcca
    44641 aaaaataatt gttttatcgc caactaatgc ttggtcatgg gattcacaat atcctatatt
    44701 tttttctcca ggtcgtctag tagtaagttc ataccacatt tttcccaaat cccagtcttc
    44761 atcagataag ttagagatag tttttatcca aaaatcaggt actcccattg caagtctata
    44821 gtcaaatcca attccgccgt cttttatagg gatacacata ccaggcattc cactcatatc
    44881 ttctgcaata cttatcgaat taggtttaat ttcttttatt aattcatttg caaattggag
    44941 atatgttata gcttcaatat ctgtattcat actaaaatat ttttcataac tatcaaatga
    45001 aactccaaga ccatggttat gatataacat tgaagttaca ccatcaaacc taaagccatc
    45061 aaaatggtac tcatttaacc aaaattttat atttgaaagc aaaaaatgta ttacttcagg
    45121 tttaccatag ttaaaaagtt ttgtacccca agcaggatga tttccctttg aacctgagtg
    45181 gaaaaattga tgttcacttc catcaaactc atttattcct tctaaagtat tctttacagc
    45241 atgagaatga actaaatcta ataaaacagc aatccccatt gaatgagcag tgtttatgag
    45301 gttttttaag tcttcaggag taccaaacct agatgagatt gcataaaagt ttgaaacttg
    45361 gtatccaaaa gatgcataat atggatgttc cataatagcc ataagctgaa tggtattgta
    45421 tccagctttt tttatcttag gaagtatttt ttcagtaaat tcattataag ttccaataga
    45481 ttcgctttca gtagacatac ctatatgaca ttcatagatt agtggagaag ttatgttttt
    45541 taagtcaaaa ttattgtcag tccagataaa tggagttttt ggttgccata tttgtccatt
    45601 aaatccacca aaatcttttt gaattactct ttttatatat aatggtatac ggtcaaatgt
    45661 ttttccattt gcagtaactt gtacctttac ctctgatttg tgaggtaagc tattttgacc
    45721 aggaataaat atttcccaat taccagaccc aatttttctt agaggatgag attttctatt
    45781 ccaattatta aagtcaccaa ttagtgacaa actatctgca tttggagccc attccctata
    45841 gaagaatcca tcatttgtct gatgaaatcc atagtaaaga tgagcatttg caaaagaact
    45901 aaaatcttta tagtttttca atgtttttga tttcatatcc tcataatgtt tcattcgctt
    45961 ttttatatca gattcaaatg gttgtaggta tacatcaata tctaaaattt taaatctttt
    46021 ttgacttttc attgtatttc tcctccataa taaatattac atctaaaaaa taatatttat
    46081 ctcatactat cttctctgta taattgatat atattatata atcctaataa aaagcttaag
    46141 ccaccaatga ttaatttcat aatttacctc cctagtattt atacttatat atcgtattca
    46201 tcaaaattta aaagtttgtt taaaactctt ttaataatat cataatcaaa gcctttatag
    46261 gaaagatgtt gagaaatttt ttgatatatt ttctttttat cttctttctt tactctttca
    46321 tacctttttt ttgctagata catagcattt tcaaattcag tgtccttgtc taattcagat
    46381 actgcttcat tgattgtact tctattgata cctttattat ataaattctg cttaattctg
    46441 tttttgccac atttatttag gttaacattt gtgttaacaa ttttttgagc taatacactg
    46501 tcatttacga gattataact tttaagaaaa tctatgaccc tctcaattat attttcatca
    46561 aattctgatg atagtttttc ttttaatttt ttttcagatt ggtcagcatg agataaaata
    46621 tttaatgctt tatttttagc ttttatatac atttcatcat ctaaaatagg ttttaattct
    46681 tcttcattta tttcattccc cttttttaaa ttaaatgtat aaacaagttc tttaaatata
    46741 gcaataaaaa atttatcatc tacataaata ttaactctat catcatttct tttttgttgt
    46801 tctatttttg taattatacc cataagtcct ccttttttat atgtaattat attaacaaaa
    46861 gcaactaata aaatttaaca aaaaacttat atttgtatgt tatattttat caaaattaag
    46921 acaaaatagt aaaaagttat ttttattaaa aagtaaagta gaatcatgtt aaataattat
    46981 ttttttaagt gttttttagc atataaatat aattatatac ccaaaaaaac aaattataag
    47041 ctttaagaaa taaaaaataa tggtacaata gtattataac attaaaagga gacaaaatat
    47101 gagaagtgaa aatctagtta agatggcaga gttgaaaaat actgaaaaac tagagaaatt
    47161 taatcgccat aaaggcaaaa tcaaaatagg tatattggga ggtacttttg accctataca
    47221 ctatgctcat ttagcaacag cagaatttat tagagataag tatgacattg acaaaataat
    47281 ctttatacca tcaggaaatc caccacataa attatgtatc actacagata agtatgatag
    47341 atataatatg acacttctag caactgaaag caacgaagat tttttagttt caaaagtaga
    47401 aatcgaaaga aagaaaagga cttatacaat agatacatta aaatacttaa agaaaaaata
    47461 caaaaatgca gacatttatt ttataacagg tgcagatgca atttgtagtg ttgaagagtg
    47521 gaaagatgta aagaaaaact ttgagttagc tacttttata gccgcaacta gaccaggaat
    47581 tagtttgtta aggtcacaag agactataga aaaactaact aagaagtata atgctgacat
    47641 tataacagtt tatgttccat cactggatat atcgtccaca tatataaggg aacagctaaa
    47701 tgaaggcaag tcaatacgtt acttagtacc cgaaaatgta gaaaattatt tatatgaaaa
    47761 taaactatat caatatggag atgattaaat gaacttagaa aatattaacc aaagactaaa
    47821 tcaaatgcta ccagtgggga ggcttagcca ttcaaaaaat gttgcaaaat gtgctgaaaa
    47881 attatgtgag atttatggat gtgacaaaga aaaagcttac ttagcaggaa tgattcatga
    47941 ttgtgcaaag tatttaagtg acaaggaaat agaggattat gtaaataagt atgaaatata
    48001 tttagaccca atagaggatg gaaatcgttc cttgtctcat agtgtaattg gggcgtacat
    48061 atgcgagtat gaatttgaag tagaagatga agatattata aatgcaatca aatatcatac
    48121 aacagggaga gaggacatgt ctcttttaga aaaaataatt tacatagctg atttaattga
    48181 agaaggaaga aaattcccag ttgtagatac attaagagaa ttagcatatg gagggaagct
    48241 tgatgaagca cttttaactt cttttaataa tactttaatg tttgtcatca ataaaaaaga
    48301 agagatacat ccaagaacag ttatggcaag aaactatcta attaaagaaa agttattata
    48361 aaaaagctat ataaaggcaa acatattaat aaatttaaat ttgactatgt tacaaaatcg
    48421 tattaatatg ttacaataac taagtgtatt ttaattagga aagtgaggaa tttttatatg
    48481 acagtggaac aaatgacaaa aatagcatat gatgccatag aagataaact gggtcaagat
    48541 acagttataa taaatatagg aaaagtttct agtttgtgtg attattttat tattactaca
    48601 gcatcttctc agagacaggt aaaagctatt gctgacaatg tggaagatga attagcaaaa
    48661 ctaggattag agccaagagg taaagaagga caaggaactc aaacttgggt actccttgat
    48721 tatggagaca taatggttca tgtatttaat gaggaaaata gagggtttta taatttagaa
    48781 aaattatgga aagatgcacc ttatatagat attgacacat tagcataaac atgataatat
    48841 atgttattat aattagaaaa tttcaaaaat tatattaaat tgaatagact agagcagtaa
    48901 aagattatat tttttcagag agttagtgga aggtgagaac taatattata taactttgaa
    48961 cttactagat taaaatctat tctccatggc tggattaaaa gctatctaag agagtctata
    49021 tatgtagtag gctaattagg gtggtaccgc gaagatttat cctcgtccct aaacgtaagt
    49081 ttagtgacga ggatttttta ttttcaaaga attatttgga aattataagt aaataatact
    49141 aaataaggta gttatacaca attaaaaata ggaggcaaaa tatgagcgta tataatttta
    49201 aagaagtaga atcgaaatgg caaaaaatct ggaaagataa taatcagtat aaaatggata
    49261 ctgcacaaac tgaaaaacct aattattaca cattagaaat gttcccttat ccatctggaa
    49321 aaatacacat gggacatgtt agaaattatt ctataggtga tgttgtcgca agattcaaaa
    49381 aaatggaagg atataatgta cttcatccaa tgggatggga ttcttttgga ttaccagctg
    49441 aaaatgcagc tataaaacat ggaatacatc cgcataaatg gacaatggaa aatatagaag
    49501 aaatgaaaga gcaattaaat ttattaggga ttagttatga ttgggataaa gaagttgcaa
    49561 cttcaactcc agaatactat agatttacac aagaaatatt tttgaagttt ttagagcatg
    49621 ggcttgcata taagaaaaaa tcatatgtta actggtgtcc ttcttgtgag actgttcttg
    49681 caaatgagca agttgttcaa ggagcatgtg agagatgtaa agcaacagta ttaaagaaag
    49741 atttagaaca atggtatttt aagactactg aatttgcaga agaattactt aatgacttag
    49801 atacattaga tggatggcca gaaaaagtta aaacaatgca aaaaaattgg ataggtaaaa
    49861 gtacaggagc agatttagtt tttgatatag atggaactga taaatctatg acagtattta
    49921 caactagacc agacacaact tatggagtta cttatatggt acttgctcca gagcatgaat
    49981 tagttaaaga attggttgca gggactgaat atgaagcaga tgttgaagct tttgttcaaa
    50041 aaatgcatac tatgacagaa atagaaagaa cagcagctga tgtagaaaaa gaaggaatgt
    50101 ttataggtag atatgttata aatcctttaa atggcaagaa agttcctcta tggatagcta
    50161 actatgtatt agttgagtat ggaacgggtg ctataatggc agttccagca catgatgaaa
    50221 gagatagaga ttttgctgaa aaatataatt tagacataat tgatgtaata acagaagata
    50281 acaaaatgat aaattcagaa gaatttaatg gattagatgc ttcagaagga tttgaaggaa
    50341 taatcgataa gttagaaaaa gaaggtagag gaaagagaac aattaattat agacttagag
    50401 actggttagt ttcaagacaa agatactggg gttgtcctat acctgtggtt tattgtgatg
    50461 aatgtggaat agttccagtt aaaaaggaag atttaccagt tcttttacca acagatgttg
    50521 aatttactgg aaaaggggaa tctccactta cgacttcaaa gcaatttatg tcaacaactt
    50581 gtcctcactg tggaaaacca gctagaaggg aagttgatac tatggataca ttcgttgatt
    50641 cttcttggta tttcttaaga tatgtggata gcaataatga aaatgaacca tttagtaagg
    50701 aattagttaa tagatggcat ccagtagacc aatatatagg tggagtagaa catgctataa
    50761 tgcatttact ttatgcaaga tggtttgtaa aggcatttaa gagtatggga atggtagact
    50821 tcaatgagcc atttaaaaac ttacttactc aaggaatggt acttatggat ggttctaaaa
    50881 tgagtaagtc taaagggaat acagtatctc ctatggatat aatagatgag tatggagcag
    50941 atactgcaag acttttcgta ttatttgcag caccaccaga aagagatttg gactggtcag
    51001 agcaaggtgt tgatggatgc tttagattct taaatagggt ttatagattg gttgacgagt
    51061 tagcagatgt ggttaaaaag gatgtagagt ttggagaatt aaattcccaa gataaggata
    51121 tgagatatac tatacactct acacttaaaa aagttactgc tgatttaagt gaaaaattcg
    51181 gatttaatac tgctatatca gctcttatgg aattaataaa tgatatgtat aaatataaag
    51241 agttagataa tataaacgaa gcagttataa aagaaggtgt acaaactata gtaactataa
    51301 ttgcaccatt tgcccctcat ttaggtgaag aactatggac tatgataggt aaagaaggaa
    51361 gtgtatttga tatagactgg cctaaatatg acgaaaaagc tttagtaaaa gatgaaatag
    51421 aagttgttgt acaagttaat ggaaaagtta gaggaaaatt aactgttaac tcaaatatat
    51481 ctaaagatga gatggaaaag gtagctttag aagatgaaaa aataaaagga ttagttgaag
    51541 gtaaaactat agttaaagtt gtagctgtac ctaaaaaatt agtaaatata gttgttaaat
    51601 aactcaaaaa tatgaacaaa aatataaatt tataaaataa atgaggctgt ctcaaaatag
    51661 agataagcct tttttcattg ttgaaattat aaaaatagat ttgattttat acaaaaaaat
    51721 gattaaagaa ctaaaaagtt cattcttata taccctagtt gttgttttaa atattattat
    51781 aattaatgtt aaatcgagtt aatgtattaa gtggtatgta aatcattcag tttccaaagt
    51841 gttgttcatc aaggaactgc tctaagctta aacgaaaaaa atataattat tgtattttga
    51901 aatttttttc tatataatga aaacaggcaa aaaaggtgca tatctatgct ttatatgaaa
    51961 cacccagaca acgaaattca gattcatata atagctacca tgtttctaaa aaggaacttg
    52021 taaaaaagat agcgtttcaa cttcccagtt gattaaaatt atcagcagga aaacaagtat
    52081 ggtcagcagt ggtattctaa tgggtgttgt cgatatatcc aagttgggat tgtctgagga
    52141 tgttatcaaa taacttgtga taggtgcagt agatgtggag attcctgata cggatttatt
    52201 ctcaggggat ggttgcttcg ggagatatgg agcgtaacca gtttattgat atggtaacca
    52261 ttttttgtct aatttgatga aagaatccca agaataccat acagaagtaa gacaggaatt
    52321 aactggtggt ataaactgat aaaggcaaat ttaaaaattt tgataggatg attttaagat
    52381 ttatcttcta ttttgttttt tagaaataat tgaaatgtta tgaggaggta tgtacaaatg
    52441 gagactgtaa caaataatca atcaggtgta gaaattcaac tcagtctgtt tgaagctatc
    52501 aaagatttat tgttaattaa tcctattttc ttgttgggaa ttatcttgat tggtattttc
    52561 ttagttgtaa tattcaagaa aaatagtgat attccaaagg taaaaacatc tattttatca
    52621 ctggcaatgt attactactt atgtttaaca ctatcacata ttgttggtat acctacattc
    52681 ggtgaatata taagactgtc acagttaggg gaaacgttct ttaatccaaa cataaatctt
    52741 attcctttta gtgatggatt tagtttgagt tttattttga atattttact ttttgtccca
    52801 ttaggttttt tatgtccgct tataagcaaa acctttgaac gtttgagaaa tacattcttg
    52861 attggttttg gattatctct ttttatcgaa actattcagc tttttacgtt acacagagca
    52921 accgatatag acgacttact cacgaatgta attggaacaa taataggcta tttctgcttc
    52981 aaactgattg ctaaattgcg aattgtaaaa ctacattcag atgatgactc tatggaacag
    53041 gattacacag tatgtattcc gattgccatt ataatagttg cccttatatt aggatttttt
    53101 agttaagttc caaatagcta atttgggaat ccagacaaca taacagatag tatgttacct
    53161 tttacaggag catactatct gttatattgt caacatccta ttttatggtt tgtgtgtagt
    53221 ttcctgtaaa ctgaactaaa taattaaata gaagctatta gaattaataa taacagatag
    53281 atattaataa gcataaacaa atagtaaaat gtgtagatat agacgttttt aagtgaaact
    53341 attataaaat aattgactga taatcaactg acaaaaccta tttttttagg tttaactgac
    53401 atttaactga cagtcagttt ttactattta aatagaagtt cgcttagatt gtcggaagct
    53461 tctttgtcca tttctttaag tacatgagaa tatctattca tagttatttt tatatctgta
    53521 tgacccaatc tttcagaaac aactttcata ttagttcccc ctaaaagcaa cagagtggca
    53581 cttgtatatc ttaaatcatg tattctaatc ttttttaaat tatttttttt gtaaaattgt
    53641 gaaacgtttt tcttagtgaa gaaggtagaa acggatttaa attggtgttt agacaaacta
    53701 agttatttct gttttccagg ataccttaca attttaattt gctttgtctt agtttttcaa
    53761 ttttcaattt ttcaattaat tcttttggag ctgataaagc tctttttgat tttggtgttt
    53821 ttggctcttt aaaagatatt ttgccattag aatagacaag aatttgatta acatttatca
    53881 gattgttatc taagtcaaca tctgaccacc taagcccagt agcttctcac attcttaaac
    53941 ctagaagtaa cattaaaaga ataggtattt ctatattatt accaagtaac ttttctatta
    54001 gcaataatac ttcttctcta ttgtatattt catttgtaga atatttttct ttagaaggaa
    54061 gttttataaa atcacatgaa ttttcttgta tttctcttaa tctatacgct tcccttatta
    54121 cagaacttag aaagctatac cttactttag cactagtaaa ggtagaatta ttaaagttat
    54181 aatttacaaa agcttgtact gaactaggat ttacttctgt gagttttaga ttcttaaaaa
    54241 aggttctatg taatttttaa cccaagattt tgtatttgta acagtatatg gagaccaatt
    54301 atttttattc atgtctatat atttataaca tctttctaca aatgtcatac cttttggagc
    54361 aacaaactta ttattattta tagtagactt tatttcaatt aaatgttttt cagcatcctt
    54421 tttcttttta tagcttccat aacttttttg tttctttttg ccagtttctt attccacata
    54481 tacatagaat ttttcccatc tttttcttat aaaagctgat ttgatattca tatatgtacg
    54541 ctccctttat aagttagttt tataaaagaa cagttggact gctctaatta tgtcattatc
    54601 tttttaattc tttacagtta cattataaca cgtatttgta tacacttaaa ttaacaggaa
    54661 gataaatatc attaaaggag gaaaagaaaa tttaatgtag aattatgtaa gatatatagt
    54721 atttcatata taaaaggggg agatattgtg aaattaaaga aattattatc actagggtta
    54781 gccgtgtcaa tgtttgctgc tggtcctata tcagctaatg cacttgatac aatttatact
    54841 attcaaggaa aagataaata tgaaacggct gctcttatag ctgatgaaca aaattatact
    54901 acagctatat taattaatgc agacagcaca atggctgatg gattaagtgc gagtggactt
    54961 gcaggagcaa caaatgcacc aattttttta actaagaaaa ataacatacc taatgcaact
    55021 cttaaaagag tagagaaagc taaaaaggta tacataatag gtggagaaag ttctattgat
    55081 aaagcaacag aaactcttct aaaagataaa ggaatagagg ctaaaagact tcaaggtagt
    55141 gatagaatta agacaagcta taatgttgca aaagaaataa actcaattaa caaagtaaat
    55201 aaagttatat tgactaatgc ttttaaagga gagcctgatg caatgagtgc tgcacctgta
    55261 gcagttagag ataaagcagc tatagtatta actgatggaa aaagtgttcc atttaataca
    55321 actggtgtag aaagttatgt aataggtggt acttcatcaa tgagtgacaa actagttaat
    55381 gatactaatt caactagatt aggtggagta gatagatatg atactaataa aaaaatagta
    55441 aataagttct ataatggcgc aaaagaattt tatatagcta gtggtactga tttagtttat
    55501 gctttagttg cttcaccaat ggctaaaaat aatcctattg tactagttga tattggaagt
    55561 aataaagata tacttaaaaa tgctgcaaaa gtgactggga taggaaatct aagtgataaa
    55621 attatgctag agtgtgaaaa taccatcaat aatattagtc ttccgtcttc aaataaaaat
    55681 ataatatgtg gattaaagat tgattttgat tcaaagcaca taatttggca agataaagga
    55741 agttatcttg aatgttatga tgataacctt gaatattata ctgaaaaata taaagaccca
    55801 acagttccta taaatataga aaatacaagt acacaaaatt tagatgtaac aaaattaaaa
    55861 gtttatggag aagctaattc aacattatat atggggcaat atgctaaaat aaaaatggat
    55921 tttaatagtt tcggtgtatt aaagccagga gaaaaatgta ctatttattt agatataatt
    55981 tcattagatc catcaagaag catgacatta acattttctt atgtaggtga taaagaaaat
    56041 tataaatatg aacaaggatt ggcaagtaga atattatttt ataacccaga tggagaagat
    56101 ttttttgaat ataaataact agtgggtatg taaattaata agggttttat aagtttaatt
    56161 tttttatttt cgttttatat taactatgtg gtatgtaaat gtctcgatat tgctcatatg
    56221 ttgagttatt atatttagtt tcatatgaac tatatggatt ttaaaattaa aaatacttaa
    56281 aaacatttac tttaatagta ggtgtttttt tgattaaaag aatattatta taatgtaaaa
    56341 agtaataaat aggtaaaata tgtaagaatt atatgttata atatttttag caagaagatg
    56401 taatctacaa tttatagagt ggagttcatg tatcaaaaaa ttatcctccc gacgctaaga
    56461 agggaggtga atatacatgg ataatttttt acaaggcata ctagcaagtc tatctgctag
    56521 cttaatagtt tatatagcta gcaaactatt tagaaagcgt aaaaaaccac tcaaagcggc
    56581 aactaagagt ggttgggaat ttgatttaaa aatcagattc cataaaacta agtaatttct
    56641 aaattatgaa ctccactcta cagcaaaata gattgtagtt cttcttgctt ttattatatc
    56701 acaaattggt acagatattc aaaaataata tttttatgat ataataaagt catagaaatt
    56761 ttgcagtgtt cgattttttg aagaaattaa ggcttaacag ttgaaatata aggcattgag
    56821 gacttgtgat aagtgttatc aattgcacta ctcatggttc actgcaaatt tgagagattt
    56881 gtatatgtgt aggcattgga aatactcaat ttattttggg gttttatatt aactatatgg
    56941 aatgtaaatg agttctgcca attcggatta tatttttgaa gtaattgttt tatattaact
    57001 atatggaatg taaattgtat ccaaaaaatt acaataatca tacgaactgc taatataagc
    57061 agtcaatata tgtgtacatt tatccatttt atctctaaac atataaatta ttatcccctt
    57121 aaaaaacttt cataaaaaca aaaagaacaa tatctctttg gcaactggct aacataactc
    57181 ataagatatt tatataagtc ttagtcccta tagctttgcg tcactagatt tctctagttt
    57241 tgccgattta gttttattct ataactaaat aatacaatag aattagttat tattcaacaa
    57301 gattgtttga aaattgaata aatttatagg tatgaacgta atttcacact taaaaacaga
    57361 ctaaatacgt agaatatata gaaaaattta cctgtagttt ttgtgataaa aagctcctat
    57421 tattaattta agctagataa gtttgaggaa gtgacataaa ttgaaagaag gaatatatta
    57481 tgttgaataa aaaattacca gatgcagaat taaaaattat gaaatacata tggaatactg
    57541 gttataaaac tgtaatatca aaagatgttg cagattaaat agaaaaaaca tatgcttgga
    57601 agcatacaac tacaattaca cttttagcta gattaactaa aaaaggtttt ttcatatcgc
    57661 agagaattgg gaaacatgta cactatagag tattaataaa agagaaagaa tatctcaaaa
    57721 tagagggaaa aagaattttt ggaggtctac ataacaatcc attatcagaa ctaatttcaa
    57781 aattacatga tggagaagaa ataactgaag aaaaaataat agaaatagga aattggataa
    57841 aaagctggaa agatgaagat taataaataa aaggtagcta ctaatataag tgctaccttt
    57901 tttcttgtgg ataatattgg ggataagttg tggaaaattt gtgtaaaaat tgtggataaa
    57961 atattttgct gacatttaac tgacataagt attagatttt gatttaaaaa tgttgaaata
    58021 tcaataaata attaaataga agttattaga attaacaata gattcctttt tcaaagaaat
    58081 ctttgatttc atcaaggtta tcagtttttc caagtgctaa gataagtttt attctagctt
    58141 tttgacctgg taagtcccca ccaaatatac aaccaagatt ttttaaatct ctaccagaac
    58201 caaagtaacc ataactatcg aatactctac ctgaatgaca tctagaaact ataactacag
    58261 gaagtccttt ttctctagca tattcaactc cagaaagcat ttgaggagga atattacctc
    58321 tgcccattgc ctcaataact atacctttat aaccactatc aacagcaaat cttataaagt
    58381 cagcattttc tcctacatat gctttaaata aggcaacttt agattctacc ttatcagtat
    58441 ctataattgt tctatttaca atatctcttg taaggacaag ttcgttgcag tcaattattc
    58501 ccaatgggcc actagttaga gatttaaatg tatttagaga aagagtattt gttttagtag
    58561 cttcagaagc taataatact tcattgttta aaactacaag gactcctttt cccatagcat
    58621 ctttggatac tgcagtacaa actgatgcag ataaattact tgaaccatca tatccaagtt
    58681 cagaactact tctcatagca cctgtgacta ttacaggttt gatgttgtca attgtaaggt
    58741 ctagaaaata agctgtttcc tctaaactat ctgtaccatg tgttattaca acacctgtaa
    58801 tatcttttct tgctaataaa tcatttacat agtttttcaa ttccatcatt ctataaggtg
    58861 ttatgtgagg tcctggaatt tcatcaaaat taaaaacttc aacatctgca actttatcaa
    58921 tgttggtaac cattgatagt atttgttcac ctgataaagt tggtatagct gctccgactt
    58981 tatcatctac tgtcatagat atagttccac cagtaaaaac tatggcaact tttttcttaa
    59041 tactcatact attctctgcc tttcataaat aaaatttata attccgatag gattatatca
    59101 aaaaaagtag ttaaggtcta cataaactct tttattcata taatttagat atttcagtgt
    59161 aatataatta tttcttaatt ttttgccatt caacgatata accatttaat atctctctta
    59221 tggcttttcc tatcttagta taagcttcat catatagatt tgctagagat attctaattg
    59281 accattcagg accatagaat ccatttccac taagtaaaac tactgaatag tcattagcta
    59341 atttataaag aacatctact ggagatgcat ttttacttat aaatttaaat aagccttctc
    59401 cgtagtttaa tttagcccat tcttcaatat caaattgagt gtaatatgca gcattgtttt
    59461 tgttttcatt aattggaagc tctaaagctt caaataatag tttttgacgt ctatggcaga
    59521 tactcatatt gagttttttg taggcatcta ctttgtcaat caatgcaaaa gcagagaaaa
    59581 acgccatttg tacttgctgt ggagtagaaa gaccagcagt atggttgaga gcaacaagtc
    59641 tgctatcagc aacaactctt tccataaatg aaaggctaga agggttaaga ctcatatcac
    59701 tgtaacgctt atctactgat ttttttaatt ctccagttaa atcatttatt ttcttatcaa
    59761 aaacattttt cttatgaagg gcaaatgtac caagtctcca tcctgtaact ccaaaatatt
    59821 ttgaataaga atatactcca acagtgttgt aaggtagttt tgacattaag gatgaaaaat
    59881 cttctacaaa agttccatat acatcatctg atattatcat taaatcttta ttataatttt
    59941 caattacatc tattagatta ttacaagatg tttcatccat agctatagaa gcaggattat
    60001 taggattaac aacgaataaa gctttaatag atttgtcacg aagtttctct aattcttttt
    60061 ttgtatattg ccatgtgtga gcaccttttt catcaacttc attagcattt atatttacca
    60121 ctttaaagtc gtaacgagga aggttaggta tttcaagata aggtgtaaat attggagtca
    60181 ttaaggctat tgtatctcct ttttttagaa gttcatttgc cattaatgaa tcaaatatat
    60241 agcacatagc agctgtagca ccttcaacag caaaaatttc tacatcacca tatttacctg
    60301 atatatcata tttcatttct tgaactaggt aatcgtgtac tattttttcc atatgaggta
    60361 acattctatc tggaagtgga tagttatcac caataattcc atcagctagt tcatgaacaa
    60421 attcatctcc atcaaatcca agttcattta taccaaagtt tataatatct ctagctaaat
    60481 atgctccagg catatctata ttttcattta taaatttaaa aaatctttct ttaataccct
    60541 ttttttgagg cattccagcc aaatgaccta aatcccaatt agaacgagtt tcagtaatag
    60601 cgaaatgacc aaaagtaaaa aaggcttctc tagcagttga acaagtccag tttggatttc
    60661 cacgacctgc atcaagtaat tctctgttat tttcttttgc agtgtatttt gcaatatcta
    60721 ttaatttgtc cttaaattca aaagggctta tttttccata tatgtttttg atttctttat
    60781 aagtatgatt tttcatgtta aattcctcct tatgtaataa gtttcacatt tttataataa
    60841 aataaacaag ttgcaaaaat gattatactt taattataat aggtttatag aaaaagtaaa
    60901 gtatgaaatt aaaataataa ctctatttag ccttattaaa tatatgttaa aataaaaaat
    60961 accttttaat aaaaaatgat tgttatataa aataaatatt caatttaatt atcaccttag
    61021 tttttttatt gtattttatt atatatgtat agttttctaa ttttgaataa aaataaagta
    61081 aaa
//
LOCUS       DBGJFF010000005        59364 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.005, whole genome shotgun sequence.
ACCESSION   DBGJFF010000005 DBGJFF010000000
VERSION     DBGJFF010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 59364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59364
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.005"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(90..863)
                     /locus_tag="ACOIAF_000406"
     CDS             complement(90..863)
                     /locus_tag="ACOIAF_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HGZ2667441.1"
                     /translation="MKNVANATHYLNMDTKELFNLYNKNKNVDIRNILIERHLYLARL
                     LAKKYINKGVDFEDIYQVASLALIYAIDRYDVEKGFEFSSFATPTIVGEIKKYFRDKV
                     WTLRVPRRIQELSKKISDAKIKLEQENKKHPKVKDIADYIGVSEEDVLEAMEASYGYQ
                     PMSLDSSSNDDSEDKDITLIDKIGKEEGNFGSIEYEDFINKFIETLNELEVKIFKDRF
                     FFDKTQSSIAKELGISQMTVSRLEKKVVEKLKKEYEKNL"
     gene            complement(863..1270)
                     /locus_tag="ACOIAF_000407"
     CDS             complement(863..1270)
                     /locus_tag="ACOIAF_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGZ2667442.1"
                     /translation="MACETIKMEITTNPDYVSIIRLTASGIANKMGFPIDDIEDIKVA
                     VSEACTNAIKHSKDDVFNIVFNILDNAINIEIQDNGKGYDVSSISTPDLENPKESGLG
                     LFIIKTLMDDVDIESEHNQGTKIKMTKYLGVDI"
     gene            complement(1274..1591)
                     /locus_tag="ACOIAF_000408"
     CDS             complement(1274..1591)
                     /locus_tag="ACOIAF_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HGZ2667443.1"
                     /translation="MSMNIDSNLDSQNKFWNVCLDGELDVSTADKLKEHLHALIEKNM
                     LDVKINLKDLDYIDSTGLGAMIGVLKKLKINEKEIYIVNPKSNVRKIFTITGLDKIFK
                     VEG"
     gene            complement(1603..1863)
                     /locus_tag="ACOIAF_000409"
     CDS             complement(1603..1863)
                     /locus_tag="ACOIAF_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HGZ2667444.1"
                     /translation="MNAWGWQIGAVLVGSSALIVAIYLAKTLNSINKVVEKAYKIVDY
                     NERHIQDIVENASSISKGIDDIVYVINKILSIGNIFKIIKRK"
     gene            complement(1863..2159)
                     /locus_tag="ACOIAF_000410"
     CDS             complement(1863..2159)
                     /locus_tag="ACOIAF_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HGZ2667445.1"
                     /translation="MCNKRSCKKGGFLLLGAILGFIFGMFFAPKKGSELRKETKEKFN
                     DVKENPKEVLHETFNDVKERIINLVDDDNNEEDIKISEEDIVISKSFDDEGDVN"
     gene            complement(2228..4654)
                     /gene="gyrA"
                     /locus_tag="ACOIAF_000411"
     CDS             complement(2228..4654)
                     /gene="gyrA"
                     /locus_tag="ACOIAF_000411"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HGZ2667446.1"
                     /translation="MEENNKILPIEIAEEMKKSYIDYSMSVIAGRALPDVRDGLKPVH
                     RRILYSMSELNLTPDKPYRKSARIVGDVLGKYHPHGDTAVYYAMVRMAQDFSTRALLV
                     DGHGNFGSVDGDSPAAMRYTEAKMSKLSLELLRDIEKETVDFKPNFDESLKEPSVLPA
                     RYPNLLVNGSNGIAVGMATSIPPHNLAEVIDATVYLIDNPECSVDDLIKFVQGPDFPT
                     AAIIMGKESIAEAYRTGRGKVKVRSRAFIEELPKGKQQIIVTEIPYQVNKAKLVERIA
                     ELVKEKRIEGISDLRDESNRNGMRIVIELKRDANANIVLNNLYKHSQMEDTFSIIMLA
                     LVDGQPRVLNLKQILYHYIKHQEDVVTRRTKFELNKAEARAHILEGLKIALDNIDAVI
                     SLIRASKTGQEAKLGLIEKFKLTEIQAQAILDMRLQRLTGLERDKIEAEYEDLIKKIN
                     RLKEILADERLLLNVIKDEITIIKENYSDERRTEIRHAEGEIDMRDLISDEEIAITLT
                     HFGYIKRLPSDTYKSQKRGGRGISALTTREEDFVRHLVTTTTHSRLLFFTNKGRVFKL
                     NAYEIPEGKRQAKGTAIVNLLQLSADEKIATLIPIDGNDENEYLLLATKKGIVKKTKR
                     EEFKNINKSGLIAIGLRDDDELIGVELTDGKQEVLLVTKEGMSIRFDENDIRYMGRTA
                     MGVKGITLSKEDFVVSMNLCSKGTDVLVVSKNGFGKRTNIEEYRSQIRAGKGIKTYNI
                     SEKTGTIVGADMVNEDDEIMIINSDGVLIRIRVNEISLFGRVTSGVKLMKTNDEVNVV
                     SIAKINIEEE"
     gene            complement(4674..6575)
                     /gene="gyrB"
                     /locus_tag="ACOIAF_000412"
     CDS             complement(4674..6575)
                     /gene="gyrB"
                     /locus_tag="ACOIAF_000412"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HGZ2667447.1"
                     /translation="MKQEYGASQIQVLEGLEAVRKRPGMYIGSTSPRGLHHLVYEVVD
                     NSIDEALQGYCSDIYVSINEDGSVLVKDNGRGIPVEIHPKTGKSTLETVLTNLHAGGK
                     FGGGGYKVSGGLHGVGVSVVNALSKWMVAEVYLNGKIYKQTYEKGLPTSKLEVVGESQ
                     DKGTMIQFMPDETIFDEIEFKYETLEYRLRELSFLNKGIKIVFEDKREGQEKRKEFHY
                     TGGLVEYIKYLNKSRTGIHDDIVYIDKKVDDCFVELAMQYTDGYTENIYSFANNINTH
                     EGGSHLSGFKAALTKTVNDYAKRNKFLKENDVNLLGEDIREGLTAVVSVKLPEPQFEG
                     QTKTKLGNSFMRGIVDSVTVDELGSFLEENPSTARIIVDKALRAQRAREAAKKARELT
                     RRKSVLESTSLPGKLADCAEKDPSKSEIFLVEGDSAGGSAKQGRDRNSQAILPLRGKI
                     LNVEKSRLDRILSSDEIKNMITAYGCGIGEDFDIDKARYHKIIIMTDADVDGAHIRTL
                     LLTFFFRYMRPLIDEGYVYAAQPPLYKVTKQKKEHYVYSDKELNILLDEIGRNGVELQ
                     RYKGLGEMNAEQLWETTMNPETRTLLQVTVEDAAIADEVFSMLMGDKVAPRKEFIEEN
                     ARFVRNLDI"
     gene            complement(6576..7691)
                     /gene="recF"
                     /locus_tag="ACOIAF_000413"
     CDS             complement(6576..7691)
                     /gene="recF"
                     /locus_tag="ACOIAF_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HGZ2667448.1"
                     /translation="MKLKSLQLVNFRNYKKLHLEFNGKVNLLVGKNGQGKTNIVESIY
                     MLSFGKSFRTNKDKEMVRFNSENLYIGGSFSKYNKYSLIELIIGKDKKGIRINKVPLQ
                     KIQELLGNLNVVIFSPEDLRLVKEGPKERRAFIDKEISQIIPKYYKYLTNYNKTLSQR
                     SRVLKNIHVDEALLDVYDDTLAKYGSYIYILRRDFIKKIANISENMHMNLTNGVERLS
                     IRYKNQINITDEDTIDTVYNKFLAKLSSNRPNDIESKTTRYGIHKDDLNIFINDLDAR
                     LFGSQGQQRTASISLKLSEIELIKNEVEEYPVLILDDVFSELDEARQKLLVNNLSNVQ
                     MFITSAEVSHKKIFDEKNVTIFNIENGDVISIENGGN"
     gene            complement(7709..7915)
                     /gene="yaaA"
                     /locus_tag="ACOIAF_000414"
     CDS             complement(7709..7915)
                     /gene="yaaA"
                     /locus_tag="ACOIAF_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HGZ2667449.1"
                     /translation="MTEITIESEYIKLDQFLKLAEIASTGGHAKFLIQEGLVTVNDEI
                     ELRRGKKIKSGDIVEIEGTKIKVL"
     gene            complement(8052..9158)
                     /gene="dnaN"
                     /locus_tag="ACOIAF_000415"
     CDS             complement(8052..9158)
                     /gene="dnaN"
                     /locus_tag="ACOIAF_000415"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HGZ2667450.1"
                     /translation="MKIICNQKILANRIGIAQKAINGKTTIELLKGILISTEEGQLKL
                     TGYDAEIGIETYVQAEIIEKGDVVVDARLFGDIIRKLPDSFVEIETDSENNIYINCVN
                     SRFKIKGYAAKEFPKLPELNEEDLYSIPQEILKNMIKQTVFAISQDQTKPVLMGELLE
                     IVDRNLNLVAIDGYRLAVKSCSVDSLTENIKVIIPGKTLIDVNSLLSGEDNVKVGFNE
                     KNAIFIINDTKIITRLLEGDFIDYKKLLPREHNSRVKLNTKELLNSIERASLLSQSEK
                     NNLIKLSIRDKVMAITSNTEKGNVYEEVEIDLDGDYLDIAFNSRYFIEGLKNIDNEEI
                     FIEFTTNVNPCIIKPTDDVNYIYLLLPVRISSNI"
     gene            complement(9400..10719)
                     /gene="dnaA"
                     /locus_tag="ACOIAF_000416"
     CDS             complement(9400..10719)
                     /gene="dnaA"
                     /locus_tag="ACOIAF_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HGZ2667451.1"
                     /translation="MDIVSLWDKTLQLIKGDLTSVSFNTFFKNIVPLKIHLNDLILLA
                     PSDFNKDILENRYLHLIEDAISQLSLKKYNIKFVLSEKEVADLNSDSTDLNYRVLYPN
                     LNPKYTFDTFVIGNSNRFAHAACVAVAESPAKAYNPLFLYGGVGLGKTHLMHAIGHHI
                     VSQKKDSKVVYVSSEKFTNELINSIKDDKNEEFRNKYRNVDVLLIDDIQFIAGKERTQ
                     EEFFHTFNTLHEANKQIIISSDRPPKDIPTLEDRLRSRFEMGLITDIQAPDFETRIAI
                     LRKKAQLERIDVPNEVMSYIAKNIKSNIRELEGALTRVVAYSSLSNRVISFDLATEAL
                     KDIITTSKNEEINVLRIKEKVSSVFNLKMEDFNSKKRTRSIAYPRQIAMYLTRELTDL
                     SLPKIGEEFGGRDHTTVIHAHDKVSKDIEESEEIKTKIDKIISDLKG"
     gene            11378..11515
                     /gene="rpmH"
                     /locus_tag="ACOIAF_000417"
     CDS             11378..11515
                     /gene="rpmH"
                     /locus_tag="ACOIAF_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HGZ2667452.1"
                     /translation="MSKRTYQPKKRQRSKEHGFRKRMKTSNGRNVLKRRRAKGRNRLT
                     H"
     gene            11593..11937
                     /gene="rnpA"
                     /locus_tag="ACOIAF_000418"
     CDS             11593..11937
                     /gene="rnpA"
                     /locus_tag="ACOIAF_000418"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HGZ2667453.1"
                     /translation="MDFNRTKGLKKDSDFRKVYKHGKSFANKYLVIYILKNKSDYSRV
                     GISVSKKVGKAITRNRVRRLIKEAYRLNIDEKIKPGYDIVFIARVSSKDATFKDIDKS
                     IKNLVKRTDISI"
     gene            11969..12220
                     /gene="yidD"
                     /locus_tag="ACOIAF_000419"
     CDS             11969..12220
                     /gene="yidD"
                     /locus_tag="ACOIAF_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HGZ2667454.1"
                     /translation="MNKIIYILKEISKYLSNLCIYLVRFYQKYISPLKGPTCRFYPTC
                     SQYSIEAFKKYGFVKGMYLTIRRILKCHPFHPGGYDPLK"
     gene            12252..12959
                     /locus_tag="ACOIAF_000420"
     CDS             12252..12959
                     /locus_tag="ACOIAF_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HGZ2667455.1"
                     /translation="MNIIGNFLGLILKVIFEFVNHYGISIILFTILVKIILLPLTIKQ
                     TKSTKAMQDIQPRIKEIQEKYKNKPEKQNEEIVKLYGEAKINPLSGCLPLLIQFPILI
                     GLFSVLREPVAHGVFANKAAFLAADNGFLWIKSLTSPDYVLAVFSGASAYVMQKVMTP
                     KDQLQGSMKVMTYVMAGMSFYWGFIFPAGLTLYWTVSNLFSIAQYYLIMNPLKAKLAA
                     NSKEEKVNEKKPKINKK"
     gene            12928..13557
                     /gene="jag"
                     /locus_tag="ACOIAF_000421"
     CDS             12928..13557
                     /gene="jag"
                     /locus_tag="ACOIAF_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HGZ2667456.1"
                     /translation="MRKSLKLIKSKSKEEAISKAIAELNLKAEDIEVEILENPSKGFL
                     GFIGAKDGTYEIFVIEREVDVAKNFIEVMLKNANVDAKVNVSQKDNLIMVDIEGKEAA
                     SLIGRRGETLDSIQFLTGLALNKINKDSHTRVLVDIENYRSKREESLIRYANKVAREV
                     AKTRKTKKLDYMNPYERRIIHSALQNDKYVITYSEGTDPYRRLVIECKR"
     gene            13669..15048
                     /gene="mnmE"
                     /locus_tag="ACOIAF_000422"
     CDS             13669..15048
                     /gene="mnmE"
                     /locus_tag="ACOIAF_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HGZ2667457.1"
                     /translation="MFIDDTIAAIATAPGEGGIGILRISGEKALKVAEEIFKSMSGKS
                     IEEYNKRTLIYGNIVDNENIIDEVLLAYMKGPNSYTGEDVIEINCHGGFISVKKILEL
                     ILSKDVRLAEAGEFTKRAFLNGRIDLSQAEAVIDVIKAKTDIAHEVAQNQLEGSLSKK
                     IRELRDKVTEILAHVEVAIDYPEEDIEHITYQTLKEKTDELKKDIKKLYDTAESGKIL
                     REGLKTVIVGKPNVGKSSLLNSILGENRAIVTDIPGTTRDVIEEFVNIKGIPLKIVDT
                     AGIRDTDDIVEKIGVEKSKESFTSADLIVMVLDASRKLSEEDIEILEKLKDKQTIVLL
                     NKNDLKQEIEEEKILKYVENNSIIKISALQQEGIEELQDKIESMVYKGSIKNNSSLVV
                     TNSRHKDALSKAYKSATDALIALEQSMPFDFVEVDLKNIWDYLGYINGDTVTEDLLDN
                     IFHNFCIGK"
     gene            15143..17038
                     /gene="mnmG"
                     /locus_tag="ACOIAF_000423"
     CDS             15143..17038
                     /gene="mnmG"
                     /locus_tag="ACOIAF_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HGZ2667458.1"
                     /translation="MIKFEAGKYDVIVVGAGHAGCEAALATARMGYKTLIITMSLDSI
                     ALMPCNPSIGGTGKGQLVKEIDALGGQMGLNIDKTYIQSRMLNTAKGPAVHSLRAQAD
                     KFKYHEEMKKTLEDEPNLDIAMDEVVEILHEGNVVIGVGTKLGCSFKSKAVILATGVY
                     LNSKIYMGEVAFYEGPNALGYAKYLTDSLVELGLRMRRFKTGTPARVHRDSIDFSVMS
                     LQEGDEKVTPFSFMNENIEKKQEPCYLTRTTEETQKVILDNLKRSAMYSGVIESTGPR
                     YCPSIEDKVVRFSDKTSHQLFIEPEGLNTKEMYIQGISTSLPFEVQLDMYKTIKGLEN
                     CKIMRPAYAIEYDCVDPTQLKISLEIKGVENLFSAGQFNGTSGYEEAAAQGLMAGINA
                     VRKIEGKEPFVLDRSEAYIGVLLDDLVTKGTNEPYRMMTSRAEYRLYLRQDNADMRLT
                     QKGYDIGLVKKDRYERFLNKKAAVEKEFERLKNERVTPKEVNSLLEEKGATPIKVGIS
                     LYEFLKRPEVTYELLEELGKGAGDDVSREVKEQCVIITKYEGYIEKQLKQIDQFKKLE
                     NKKLDEKINYSSIEGLRLEARQKLDDIKPISIGQASRISGVSPADISVLLIYLEQIRR
                     TRGGKGE"
     gene            17031..17750
                     /gene="rsmG"
                     /locus_tag="ACOIAF_000424"
     CDS             17031..17750
                     /gene="rsmG"
                     /locus_tag="ACOIAF_000424"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HGZ2667459.1"
                     /translation="MNNIELLKNGIEGFNIDTNDSMLEKFKIYREILVEWNKKMNLTG
                     IEDEKEVYIKHFLDSISAVKNGYIKNGMSIIDVGTGAGFPGIPLKICLDSLELTLLDS
                     LNKRINFLEEVSRVLELDNITFIHGRAEDFGKNEDYREKYDVATARAVAGLPILMEFC
                     VPFVKVGGYFICLKGPNANLELEESQKAIDVLGLEYIEKIDVELPEVDLNHNILVFKK
                     VMETPVKYPRKAGKPAKNPIK"
     gene            17913..18698
                     /gene="noc"
                     /locus_tag="ACOIAF_000425"
     CDS             17913..18698
                     /gene="noc"
                     /locus_tag="ACOIAF_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0032466 - negative regulation of
                     cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HGZ2667460.1"
                     /translation="MEDKRVMEIPIEDVVPNPYQPRKIFSQVSLEELSNSIKVYGIIQ
                     PITVRAKDGKYELIAGERRLRAAKLAELKTIPAIINNMNDESSAVLALLENLQREDLN
                     FIEEAIGYENLIKEHAFTQQQLAEKLGKNQSTVANKLRILKLPNDIKMKLIENNLTER
                     HARAFLKLPSEDLMQSVLDKVIKNELTVKKTEKLIQDVLEETKVQEEPDKKQNIKGAM
                     SIRIYINTIKQAFDAISNTGIDAKYNEIDKGDYMEVVVKIPKK"
     gene            18833..19606
                     /locus_tag="ACOIAF_000426"
     CDS             18833..19606
                     /locus_tag="ACOIAF_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HGZ2667461.1"
                     /translation="MGKVIAVFNQKGGVGKTTTNVNLSASLGTLGKKILVLDLDPQGN
                     TTSGYGINKNEVENTIYEIMLDGLHIKEAIISTEFENVDVVPSATELSGAEIELTSKT
                     NREYILKNSIKAVIDEYDYIFLDCPPSLGMLTINCLTAVDSVLIPIQCEYYALEGVSQ
                     LMETIKLVKSRLNADIEIQGVVLSMFDGRANLSIQVVEEVKKYFKGSVYTTLIPRNVR
                     LAEAPSHGKPVIYYDKRCRGSVAYLELAEEFIDLEEEEW"
     gene            19608..20471
                     /locus_tag="ACOIAF_000427"
     CDS             19608..20471
                     /locus_tag="ACOIAF_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HGZ2667462.1"
                     /translation="MENKSKRSNRLGRGLSALIPEIKGETSEKEIVNIDIDKIYPNEV
                     QPRKQFDEEKIKVLSDSIKNYGVLQPIVVKMDENNKYMIIAGERRFRASKLANKNQIP
                     AIIKDIDMKDIMEIALIENLQREDLNSIEEALAYKSLIEHYNVTQEEISEAVGKSRPH
                     ITNTLRLLNLGQDVIEMIDSGRITAGHGKALLRIADKDLQLQIAKKIEEEELSVREVE
                     NIAKKISENKQEEPKKSKPKDVFILDVEDKLRNIFGTKVNISKGKKKGKIEIEYYNDD
                     DLNSIVSMLLE"
     gene            20504..21646
                     /locus_tag="ACOIAF_000428"
     CDS             20504..21646
                     /locus_tag="ACOIAF_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HGZ2667463.1"
                     /translation="MIYLDNAATTYPKPERVYNAVLDCMKNYCANPGRAGHKLAMRAA
                     REIYDTRENIAKLFNVSNPMNIVFTSNATDSLNLAIKGVLQEGDHVITTSMEHNSVIR
                     PIKALEKRGIENTVVKCDYEGFLDYEDLEKSIKSNTKLIVTTHASNVCGTLIDIKKVG
                     EIAKKHNILFLVDASQTAGVYDIDVNECNIDMLAMPGHKCLFGPQGTGILYVREGLNL
                     NILKEGGTGSKSEEIVQPELFPDKYESGTHNTPGIAGLNQGILFIFERGINNIRQHEE
                     ELCQYMIDKLEEVPDIKIYGPKDSKKRASVIALNIGDMDSGEVTFLLDSDYNIATRSG
                     IHCSPLAHTTLGTLKQGAVRFSIGYFNTKDEIDKAVEALKKISKNK"
     gene            complement(21780..22355)
                     /locus_tag="ACOIAF_000429"
     CDS             complement(21780..22355)
                     /locus_tag="ACOIAF_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HGZ2667464.1"
                     /translation="MKNKKIMLVLSILSISIFAVGCTNAQNGSDSSKKETKSNTNVEQ
                     PKEENNTEKEVPNNKAKPTPKEETKTQSATIYSFDVDKTDLIENKVDLNKIDENTLFE
                     ELQKLKVVPESAKLNSFTTKDIDGVKTGILDVSSDFTKSNLGSDAETLMLDSVARTYI
                     KNMNVEQIKITVDGSNYESGHIVLEEGDYLK"
     gene            22615..23907
                     /gene="rstA"
                     /locus_tag="ACOIAF_000430"
     CDS             22615..23907
                     /gene="rstA"
                     /locus_tag="ACOIAF_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin production/motility transcriptional
                     repressor RstA"
                     /protein_id="HGZ2667465.1"
                     /translation="MEILSLGEKIKKLRKEKNMTLKELAGDRITAAQISHIERDKSHT
                     SYELLEYLGDKLDVSVEYLLETKEMQSKKITDNLILQSEIYIKENELNKAEDQIKEVL
                     DICKRYDLMENYGRCNFLMANINLKKSNYNDAIENFEKALYFFIKNSDSENILKCYLN
                     IGKIYMQEDFYKGAISHFNFAEEILSENKFEDVNIHKELYSKMAYCYIKLDNPNMSLK
                     YIEKINEIDNRNDRKEDVDILVLKANNMLEIGKYEESRDYFKKALKILESEDNKTGLA
                     NVYLTICDVYRKIGETDRVLEYSQKVYDIKKNDEDEYMMSGLFKIIEAYIDKEDYDLA
                     RKYCKIALASSIKNKNKFNEYKALRFYANMYKNQNEVTLAIEYLIKCIKIVSDLGNNR
                     ILANLYIDLGQLYSNISKEKELEYYQKGVFMYKNLEIM"
     gene            24012..24614
                     /gene="yedF"
                     /locus_tag="ACOIAF_000431"
     CDS             24012..24614
                     /gene="yedF"
                     /locus_tag="ACOIAF_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HGZ2667466.1"
                     /translation="MSIKIDARGLACPKPVINTKRELDNLEEGVVVTIVDNETAKENI
                     LKLAKSLSCEANIVDEKENFISIEIRKGKNILDKQNVVDNKESELDNTCIFISSDKMG
                     LGNDELGKVLIKGFIYTLTESKPYPKYVLLVNGGVKLSAENEETIENLKILENMGVEV
                     LSCGTCLDYYNLKDKLQVGTVTNMYTIVETLKNASNTISI"
     gene            24687..24950
                     /locus_tag="ACOIAF_000432"
     CDS             24687..24950
                     /locus_tag="ACOIAF_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HGZ2667467.1"
                     /translation="MNQMYIVSFNSTHHAIRSEKLFGENSLKVMALPTPREITASCGI
                     SIKFSFEDMEKIKTILVENNVDIKGIYCISKLENGSKEVEKLD"
     gene            24965..25150
                     /locus_tag="ACOIAF_000433"
     CDS             24965..25150
                     /locus_tag="ACOIAF_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HGZ2667468.1"
                     /translation="MPMDLKIGDIVELKKQHACGCKEFEIIRTGMDIKIKCTKCSRLI
                     MLDRETLEKRVKKLIKK"
     gene            25253..26449
                     /locus_tag="ACOIAF_000434"
     CDS             25253..26449
                     /locus_tag="ACOIAF_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HGZ2667469.1"
                     /translation="MAVKYAKRMQGLQGSEIRELLKLTQQPQIISFAGGMPAPELFPV
                     EEMKKVSVAVLEENGRSAMQYTTTEGYEPLREKIAARMNDKNKTNVNKDDILVTSGSQ
                     QGLDFAGKVFIDEGDVILCESPSYIGAINAFKSYQPKFIDVPTDSDGMIMEELEKILE
                     TTDRIKMIYVIPDFQNPTGRTWPLERRKKFMEIVNKFEIPVIEDNPYGDLRFEGETLP
                     SLKSMDTKGLVIFLGTFSKIFCPGYRLGWTCASPEILSKFNFAKQGADLQASTISQME
                     VSKFMDMYDLDAHVDKIKAVYVKRRDVMLKTMEEEFPEGLVFTHPEGGLFTWVELPSN
                     LNAKELMPKCLDKNVAYVAGGGFFPNGGRENTFRLNYSNMPEEKIIEGIKNIAAVLKE
                     AMGVEA"
     gene            26662..26940
                     /gene="rpsF"
                     /locus_tag="ACOIAF_000435"
     CDS             26662..26940
                     /gene="rpsF"
                     /locus_tag="ACOIAF_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HGZ2667470.1"
                     /translation="MRNYELVYVVKPNSDEEVREAILNKVKEVVATDGEIVKVDTWGT
                     KKLAYPIAKFTEGFYVLVNFKSAVDVPKEIDRNLKINENVIRHMIVVA"
     gene            26977..27411
                     /locus_tag="ACOIAF_000436"
     CDS             26977..27411
                     /locus_tag="ACOIAF_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HGZ2667471.1"
                     /translation="MNQVVLVGRLTRDPELRYIPGTGTPVASFTIAIDRDYVKKDGSK
                     ETDFIPVEVMGKSAEFCANYITKGRLVALQGSIRVDNYQTQSGEKRTFTKVSTRSVQA
                     LDSKNKSENSYRESAPAFEPSFEPQGLDPQGFQAIDDDDIPF"
     gene            27430..27657
                     /gene="rpsR"
                     /locus_tag="ACOIAF_000437"
     CDS             27430..27657
                     /gene="rpsR"
                     /locus_tag="ACOIAF_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HGZ2667472.1"
                     /translation="MMNKKRRKKKRVCQFCADKNAKIDYKSTQRLQKYITERGKILPR
                     RISGTCAKHQRELTVAIKRARNIALLPYTLD"
     gene            27800..28120
                     /locus_tag="ACOIAF_000438"
     CDS             27800..28120
                     /locus_tag="ACOIAF_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HGZ2667473.1"
                     /translation="MKIDKGSEITRNIKIIEWMKTEILMSVSDLFNLLFKGVKPLDEA
                     IQDTLANIIMITYLLAKRLGISFRDVDYKVKEKIKIGIDEDHSVERWYGDLSNLKKHM
                     DNRE"
     gene            28128..29084
                     /locus_tag="ACOIAF_000439"
     CDS             28128..29084
                     /locus_tag="ACOIAF_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HGZ2667474.1"
                     /translation="MNNKIRLSKAMSIIVLAIIIALVIAYIPMLGIFSMLAAVPYVII
                     GAITDRKYSLISILVTFCVLILFADISYALNICIMYSLPGIVIGKMLKRSFEQEDSNK
                     FEPIYGGTIIFVLSMLVYFFVLKTFLNVNLLDEVSKMISEIVNIQKNSFSATELKVFD
                     KMKPDEIVSYFTNMLPMMLFLQGLLSAFVTYYLSVFFIKRITKMNIRFPKFADFYLPG
                     NAILTTFLLYLLVLFIQIIGSKLYTELIMTNLQLVFNLMFVIQGIAVCIYFLKKWIRQ
                     GPNKIMFFSGIILCLFGFMGISFVGMVDSIIDFRKVRSYKST"
     gene            29095..31089
                     /locus_tag="ACOIAF_000440"
     CDS             29095..31089
                     /locus_tag="ACOIAF_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HGZ2667475.1"
                     /translation="MSNKQTFKLNMPEINLYIIVIGISSIILLYYNLYVGCLFFCIFV
                     YMVFHNWRTTNIRRHEWTEYIQNLSLDIDETTKKAIINLPIPLCILEFDGNISWYNGK
                     FYDMIDQKDLLDKNIEDIVKNLNLRKVLNENKEMYTEINYKEKEYTIIYNVIKNDQEK
                     NPKYLMILYWIDKTEYLKVKQNYDDEKNAMMLIQVDGYDEVLKSAAEDKRALINVEVE
                     KILSALELNSNGALRRTSKDKFFLVMHKKELKKLEAEKFSILDTIRHIDYGNNLPVTI
                     SIGIGIDGDTLNENLKLATGALDLALGRGGDQAVVKTKDKFVFYGGKSKAVEKKTKVK
                     SRLIGHALREVIQQSDQVYIMGHKYPDMDAMGAAVGVYDICKSCNKTANIVLQSVNES
                     IEIFINKINENNYYKKLFIGKEEAIDNCTKNTLVVVVDTHRPNYTECEELLKLSEKVV
                     VIDHHRRGVEFINDAVLLFHEIYVSSTCEMVTELVQYMDEDVTINKLTAEGLLAGISL
                     DTKNFAFKTGVRTFEAASYLRKVGADTIEVKKFFNSDVKDFIIKAEIIQSTKIINNRI
                     CLAYSSTEIDSINVIIAQTADELLNIKEVEASFVLGEKDDTIFISARSLGQINVHVLM
                     EKLGGGGHIDIAGAQLKNVSLKEAYKMVNKIIEEYLEEEE"
     gene            31090..31539
                     /gene="rplI"
                     /locus_tag="ACOIAF_000441"
     CDS             31090..31539
                     /gene="rplI"
                     /locus_tag="ACOIAF_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HGZ2667476.1"
                     /translation="MKVILLKDVKGTGKKGEMKEVSDGYARNFLFPKKMAVQADSVAI
                     KELNEKNKSKEIKAQKEYEEAVLLGKQMEEINIEIYSKAGEGGRLFGSITSKEIAEQL
                     KKQKDIDVDKRKILLDEPIRSLGSTFVEIKIHQKVTTKIRVDVKEKQ"
     gene            31558..32886
                     /gene="dnaB"
                     /locus_tag="ACOIAF_000442"
     CDS             31558..32886
                     /gene="dnaB"
                     /locus_tag="ACOIAF_000442"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HGZ2667477.1"
                     /translation="MEDMTRIPPHSVESEQSILGSILLDKDAIITVTETIKPDDFYKE
                     AHKIIYECMITLSNKGEPIDLITLTEELRKQGHLNDIGGISYITSLSTIVPTTSNVKY
                     YADIVKEKSVLRKLIKASNEIINLGYSGATKIEDVLEQAEKSIFDISQEKTSDDFKSI
                     NLVLMDAYDMIEKLYTNKSDVTGITTGFKDLNKKINGLQRTDLILIAARPAMGKTAFS
                     LNLVQNAALKGDASVAVFSLEMSKEQLVQRMLSSQSSVELKKIKTGTLNDNDWPRIID
                     AMAVLSDAKIHIDDTPGIKISELRSKCRKLKIEKGLDLVLIDYLQLMEGEGNNESRQQ
                     EISKISRSLKILAKELNCPVVALSQLSRAPEQRADHRPMLSDLRESGAIEQDADIVMF
                     LYRDEYYHADSESKNIGEVIIAKNRHGENGSVELVWLGEVQRFGDKLRDL"
     gene            33091..33399
                     /locus_tag="ACOIAF_000443"
     CDS             33091..33399
                     /locus_tag="ACOIAF_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667478.1"
                     /translation="MEKISLKYIYPNIIKVLDEINLFRVIDNNLRESIVVYANNVDNQ
                     YHINMTNTNFGNIINICKLEKLLDVDKFMEKVIKYEKEIIEKEEFSKIEEYMLNIGEY
                     "
     gene            33481..34770
                     /locus_tag="ACOIAF_000444"
     CDS             33481..34770
                     /locus_tag="ACOIAF_000444"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HGZ2667479.1"
                     /translation="MKTVAIVGSQWGDEGKGKVIDYLATQADVVVRGQGGNNAGHTLV
                     VEGKKYALHLIPSGVLNPNTVNIIGNGIVFDPKGFLEELEMFKTDNISTENIKISDRA
                     HVIFPYHKELDALSEEARGDLKIGTTKKGIGPCYMDKTERSGIRICDLMDKDKFAIKL
                     KAQIDAKNEIVKNIYGKEELFDFETIYNEYLGYAEQIRKYVADTSVIVYDAVRAGKKV
                     LFEGAQGTLLDLDLGTYPFVTSSHPTSGGFAIGAGIGPNMIKDVVGIVKAYTTRVGEG
                     PFVTEQINETGDKIREQGHEFGVTTGRPRRCGWFDAVIVKYAARVNGLTSISFMLLDV
                     LTGFDKIKVCTSYKMGDKIITDFPASLDDLAKCEPVYEELDGWNEDITQIDNFDDLPE
                     NAKKYVAKIEELVGVSVDMVSVGPNRAQTIIRRNIFA"
     gene            35096..35171
                     /locus_tag="ACOIAF_000445"
     tRNA            35096..35171
                     /locus_tag="ACOIAF_000445"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35129..35131,aa:Lys,seq:ttt)
     gene            35178..35252
                     /locus_tag="ACOIAF_000446"
     tRNA            35178..35252
                     /locus_tag="ACOIAF_000446"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35211..35213,aa:Glu,seq:ttc)
     gene            35257..35332
                     /locus_tag="ACOIAF_000447"
     tRNA            35257..35332
                     /locus_tag="ACOIAF_000447"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35290..35292,aa:Val,seq:tac)
     gene            35366..35440
                     /locus_tag="ACOIAF_000448"
     tRNA            35366..35440
                     /locus_tag="ACOIAF_000448"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35398..35400,aa:Thr,seq:tgt)
     gene            complement(35823..36815)
                     /locus_tag="ACOIAF_000449"
     CDS             complement(35823..36815)
                     /locus_tag="ACOIAF_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGZ2667480.1"
                     /translation="MNEDKIRKILAKYAKRRDDNELLLEHRKNEVYNRIPEIKSIDDE
                     ISKIGLSLAKIVLLNPKSKDEIVKKTKENIESLKVKKERLLAESNIPLDYLEIKFQCI
                     SCKDKGFLPNGEKCSCLKQEIVNEAYKMSNLDRILSQENFSNFNLNIFSPKKGSDGEI
                     SPRENMLNNLSICENFVHDFKKDNSENLLFYGSTGLGKTYMCNCIAKELLDKGNVVIY
                     QTSFRILDILEDYKFRRDTNNQISEDNYKNLFDCDLLIIDDLGTELNNSFTSGEIFNI
                     VNTRLVAGKKIIISTNLTPSQIGNTYTQRTLSRILDKFRILEFTGDDLRWERFK"
     gene            complement(36824..37882)
                     /locus_tag="ACOIAF_000450"
     CDS             complement(36824..37882)
                     /locus_tag="ACOIAF_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HGZ2667481.1"
                     /translation="MFFKESNEIDLGEITIPNIFIDIFMPMADGLYVKVYLLGYRQAC
                     DITSNPKFDNNSIAKNLNIPLSDVLSAWKFWEEKKIIKIHDNGEYDNFNYSIEFLDLK
                     NFYIENILSNNSSIKSNTDKVVSTSENPSIRKMFNSINKIVGRYLDPSEKMSIMDIMN
                     KYNMSPDMILCAYEYVKDKTGTSKPVKYIEGIIRNWYDSNLYTPKDVEESFLVRSERY
                     ILYKTIFNELGFSRQPSKSEKELMDTWFDKFNMDIDLIINACSKSKNISNPSISYING
                     IIKNWNEKNIKNLNDLKQKEEERIVKENINKKQINTTQNNNTYKKTKFHNFNETFTQY
                     TSDELDEIIKKSQKEKFK"
     gene            38007..39470
                     /locus_tag="ACOIAF_000451"
     CDS             38007..39470
                     /locus_tag="ACOIAF_000451"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HGZ2667482.1"
                     /translation="MVIKFNQKVRRTLSVAILFMVLIIGCFIEKDDSLETYKEKTNSK
                     INQYNMDVIFDDETKRLMCNQNVDYINNTKSNIDKIYFHIYPNAFSKKDFAPFEKSEM
                     TRAYPNGFNEGYIDIKNILNNNSKMEYKIKGDKNDILEIELGKELKPNERMSLDIKYN
                     VKIPNSIGRFGYGENTINVTNWFPIACVNDERGWNLKSYETIGDPFYSETSDFHVKLL
                     IPNKYKIAHTGKLIDDKHDNKKMLYEIEAENVRDFAFILSSKFDVNKVDSKGVAINTY
                     NLNKKLSKKATEVAKDSINIFSELFGKYPYKEFSVVASDFFIGGMEYPMLVMIDQSLY
                     NEKNEFLLEYVIAHETAHQWWYSAVGNDEISEPWLDEALTEYSTIVYFEQKYGKDMAN
                     KLIKTMEIQTKSYLSENIFKPANEYKNSTEYSLNVYTKGAIAFNEIRKEVGDKVFFET
                     LNEYYNKYKHKNANGRAFVELWNSKGVDINKIISECK"
     gene            39572..40369
                     /locus_tag="ACOIAF_000452"
     CDS             39572..40369
                     /locus_tag="ACOIAF_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HGZ2667483.1"
                     /translation="MLKYCSVGSGSSGNCHYIGYKNTNILVDAGLSGKRITTGLKDIG
                     VDADKLKGIFITHEHVDHIKGAGILSRKFDIPVFANIKTWCSMKDKLGDIKDKNMKVF
                     ENDKTYSLGDIIVRPFSIEHDASDPVGYNFYTESDEKMSIATDIGCITQNIKKHLYKS
                     KLVVLESNYDPNMLMMGSYTYALKKRVMSSTGHLSNEDAANFCVELINEGTESILLAH
                     LSKENNFPELAYETSKGVLASNDIVVGQDVKLDVLSRNDVSNVYEMK"
     gene            40382..42484
                     /locus_tag="ACOIAF_000453"
     CDS             40382..42484
                     /locus_tag="ACOIAF_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HGZ2667484.1"
                     /translation="MLKRNFENVEDNKILYSIKQGMILAIPAIMTGSTALVILNLPIK
                     AYQEYLSGLFNGEVTNILNFINDSTLGIISLIILLTISYSYGKIYGSKYTVLVPIVAM
                     CSFLVFSHGNELNSYIEIFKTKWLFTSIIVSMTSSVLFVKLTESFVTSVKFHTEGADA
                     DFNMVVSAIVPFIIVVFLFSIFRVVMISLIGSSNFQDIFYNLFSNVFNKMGTNLMSAL
                     LFIFLMHFMWFFGIHGSNVLDTVAKNLFENSMAININLVNNNQLPTEIFTKTFFDTMV
                     LLGGCGSLLCLVIAIFLSEKRINVRKLAKIASIPALFNINEMLVFGIPIVFNYIMFVP
                     FVITPIILTITSYVAMSTGIVPCTVSAVEWTSPIFLSGYMSTGSIRGSLLQVFNLCVG
                     VMIYIPFIKMSQNRYTYMFKNNIENLTNLLKRYELTGEQPNLLNHNGKIGSISKMLAS
                     DLKFAMKRGEIELFYQPQVNYNGNVVGAEGLLRWKHSIGGFIYPPLVIILAKEEGFLD
                     ELGEYIINKACIDLKKSEKLLRNPVKFSVNISPDQLDDPKLPEQIKNIISSNDINPNM
                     LGIEITEQIALSGSQIIIERINAIHNLGIKLIMDDFGMGHSSLIYLQNNNFDIVKLDG
                     SLVKEILTNKRSSEIIMTIVNLSKNLDFDIVVEYVENIDQRDKLYGLGCEIYQGYYYS
                     KAIPFEEFVEYANQEREI"
     gene            42552..43031
                     /gene="rlmH"
                     /locus_tag="ACOIAF_000454"
     CDS             42552..43031
                     /gene="rlmH"
                     /locus_tag="ACOIAF_000454"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HGZ2667485.1"
                     /translation="MNISIVVVGKIKEKYLKLGIDEFKKRLSKYCKLEIIELDDEKAP
                     ENLSVKEMEIIKDKEGKKILGKIKHNSYVIALAIDGKGLSSEELAKTMSDLAVRGNSS
                     LCFIIGGSLGLSDEVLGRADYRLSFSKMTFPHQMMRLILLEQIYRAYRINNGEPYHK"
     gene            43180..43491
                     /locus_tag="ACOIAF_000455"
     CDS             43180..43491
                     /locus_tag="ACOIAF_000455"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGZ2667486.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMESAAKENGIESQIWAIPESTIQN
                     EIEKCDVLLLGPQVRYVLPKAQEIAKPYNIPVEVINMMHYGTVNGEAVLNRAIELNNT
                     K"
     gene            43539..43856
                     /locus_tag="ACOIAF_000456"
     CDS             43539..43856
                     /locus_tag="ACOIAF_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HGZ2667487.1"
                     /translation="MDEKIIEISFNIIGYAGEGKGLAFEAIKEAKNGNIEKAKELLKE
                     SREVINKAHRYQTELIQNEASGNRTEISVILIHAQDHLMNGMNFQQLAEEIVDLHLKL
                     QDK"
     gene            44069..44770
                     /locus_tag="ACOIAF_000457"
     CDS             44069..44770
                     /locus_tag="ACOIAF_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGZ2667488.1"
                     /translation="MLRNQPLYIQVVDILKQKILNNEYKIDENIPTERELEQEFGVSK
                     ITIRKAIEILESEGYVEKKSGKGTKVISNSLFNKLSKAESFSSILLSKGHKLSKETIS
                     IEKIINDTNSQVFSIFGEKCSKITRMYYLDSKPYIFFTHYIPFDENLTVESFNNESSL
                     YMYLYRRNINITRFEDEFFIEESELEVSKALNLAKDILLGRKRKTYDENDNIVELSFA
                     RYNTEVQNYVIKYEI"
     gene            44994..46295
                     /locus_tag="ACOIAF_000458"
     CDS             44994..46295
                     /locus_tag="ACOIAF_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HGZ2667489.1"
                     /translation="MEKFASFLERKIMPVAAKISNQRHMRAVRAGIIATLPLTIVGSF
                     FTILLNIPIDAYMDFIAPFKAILDIPFRYTVGFLSLYATFGIASSLAKSYDLDTTGVG
                     MLAVMAFLVSTIVPTQVVEPVGGVIEAGRWMDMAKLNSPSLFGAIVTSLITVEIYRFM
                     KEKNITIKMPAGVPVEVSNSFTALLPTAVILMLFWVIRHILGFDISVALSAMLSPLKG
                     FLSGNSLGGGLLTVFLITLFWVLGIHGPAIMGPVIRPIWDMSIAENITAFTEGVSAHA
                     MPNIFTEQFLQWYVWIGGAGATLALVVMFMFSKSQYLKSLGRLGFLPGLFNINEPIIF
                     GAPIVMNPILGIPFIIGPIIMTILSYVLTITGVIPMMVARLPFAMPAPIATVMSTNWS
                     ILAGILVLINFVISFAVYYPFFKVFEKQQLQREQEEMSENL"
     gene            46432..47526
                     /locus_tag="ACOIAF_000459"
     CDS             46432..47526
                     /locus_tag="ACOIAF_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HGZ2667490.1"
                     /translation="MERRLGISVYPEHSNMEDDKAYIKMASKYGFKRIFMCMLSITRP
                     KEEVKKHFKEIITYARELGFEVILDIAPNIFDILQISYDDLTFFSELNASGIRLDYGY
                     DGSKEAMISFNPYDLKVELNMSNDVAYLDNIITYKPNVNNIYGCHNFYPQRGTGLPYD
                     FFVRCSNRFKKYGIRTAAFVNSKYATIGPWNINDGLCTLEMHRNLDIATATKHLFATG
                     LIDDVIVGNAYASEDELKRMSEVNEYQVVFNVVAEEKTSEVEKIIMVNEQHFRRGDIT
                     NQVARSTEVRKKYAKENFPKHDNKNLFKRGDVVIGNNDFGKYKGELQLILQDCTDERK
                     NKVGRIKEDEIILLDFIEPWSKFKIILEGE"
     gene            47528..48661
                     /locus_tag="ACOIAF_000460"
     CDS             47528..48661
                     /locus_tag="ACOIAF_000460"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HGZ2667491.1"
                     /translation="MKIDILIKCGKTVDKSIIDIAILDDKIIEVKNQIDDSKYQAKQI
                     INLDGEKFISAGWIDIHTHCYERLDLYKDYPDEVGIKSGVTTVVDAGTTGALDIGKFY
                     EDTKKYKTNVYALINIAKQGITSQDELSSMMNIDEYELKRAVKKYKDFVVGIKARMSK
                     SVVISNDVEPLKVAKRIKNELNLPMMVHFGSSPPTIEDIFDYMEKGDILTHIYNGKPN
                     GILRGNEVKKEIIEARERGIILDVGHGTESFSMDIAMKSKDAGIFPDTISTDIYIKNR
                     INGPVYNLSTTMEKFIYMGYSLEDIIDKVTKNAADAISLKNKGLIKEGYDADLTIFDV
                     VNEEKELQDSLNKSVITSTSIKPRAVVVNGEYLNIGKSIGENE"
     gene            48679..49788
                     /locus_tag="ACOIAF_000461"
     CDS             48679..49788
                     /locus_tag="ACOIAF_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HGZ2667492.1"
                     /translation="MSSIFEKYSVRRVINASGKMTILGVSTVDKEVIEAIGEGCKNFF
                     IIEELINKTGEYIANLLGCESALIVSSASAGIAQSVGAMIAKDDMSLVYNVNNPKKQV
                     KREIIIPKGHNVDYGVPVEVMIRLGGGEVVEAGYANMCNKEHIIREINENTAAIMYIK
                     SHHCVQKSMLSVLDAVQVAKEYNLPLIVDAAAEEDLNVYYELGADLVIYSGAKAIEGP
                     SSGLVIGKKKYIDNVKLQSKGIGRAMKIGKENIVGLTCAIERYISNDKVTLKEMEDKL
                     NPFIDKINTIKGVSASITRDSAGREILRGEIDFNEDIINKSTQQIINELRSGEIAIYT
                     RDYKANEGKIEIDIRSVTGSDLDTIYNRIKNIVES"
     gene            49853..50593
                     /locus_tag="ACOIAF_000462"
     CDS             49853..50593
                     /locus_tag="ACOIAF_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HGZ2667493.1"
                     /translation="MNCIPQYYKNRVCLNVLAGSIKNAKEVYDACDGHVLIGVLSKNY
                     STVEVAIEDMKKYSKEIDNAISVGLGAGDPNQSNMVSQISKELQPQHINQVFTGVATS
                     RALLGQNDSIINGLVSPTGKPGFVKISTGPLSSKEKDGIVPIETAIAMMKDMGGSSIK
                     FFPMGGLKTKDEYIEVAKACAKHNFYLEPTGGIDLDNFKEIVQIALDAGVEKVIPHVY
                     TSIIDKETGETKVEDIGVLYKIMKELLG"
     gene            50598..51590
                     /locus_tag="ACOIAF_000463"
     CDS             50598..51590
                     /locus_tag="ACOIAF_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HGZ2667494.1"
                     /translation="MNILAFGEIMMRLSVPDYKFLTQTNELNYIITGTGLNILSGLKN
                     FGYNTYMLTKLPNNNVGKASSANIRKLGVKDDFITYGGNHIGVYFLENGYGERPSEVT
                     YLNRLNSSFCESKLQDYDIDKCLEEMDIVHICGIAMQLTDSVKEIALELARKSYEKGI
                     KVFFDFNFRQSLNPERTYDDLVNDYKKILPYCHGLFASYRDINKILNIDSLNIKSSSE
                     LIKKEYDIDIVAGTMRRFDDMNNRFIKGYISNDDGYFESREYKLEIYDRVGAGDGYVA
                     GIIYSYLENMKSEDIVEFGVASSVLAHTTYGDNSLVSTGHVLRLMNNEKIDILR"
     gene            complement(51778..52659)
                     /locus_tag="ACOIAF_000464"
     CDS             complement(51778..52659)
                     /locus_tag="ACOIAF_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGZ2667495.1"
                     /translation="MNTKQLEYFISVAENLSFTKTAEKFYISQTAVTQQIKALEEQIN
                     VTLFTRSKRHVELTPAGKVFLSEARTIIKNINDAIAKTQQFAHGFFGTLSIGTLIGYE
                     KNKLQQYLKEFSNTYPNISMDICTNEITELLNLVKNNSMDLAFVINPENQPLKDFEYK
                     TVERYSLVALLPPGHPLYNEDSIDLIELQHDKFIFVKETGDEYGQKSMIQNRYREAGF
                     VPNVVQRSNNLNTIESLVSSNMGISILPSFCVTDTMIERDIAIVPIKEVENRIEVVVV
                     WNKKNTNPALEKFISII"
     gene            52933..54321
                     /locus_tag="ACOIAF_000465"
     CDS             52933..54321
                     /locus_tag="ACOIAF_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LVIVD repeat-containing protein"
                     /protein_id="HGZ2667496.1"
                     /translation="MEDKFYAKGNGNNGYIKNLEVCSFNNLDGTCGMFQMALYKRDEK
                     YYLYGCCFGGNKKNGVMISDITDPYNPQFIKHFQMLDPKEYPTTTTPKIQIADDLMIV
                     AMSCGSGPGALVDQAKLANIKCEAGIRIYSLKEDPLNPKFLGYWDCGLKHVMGVHRFM
                     YNGGRYVHLSSDCVGFEGLIYRVIDIINPTNPVEIGKWWRPDQYADGYPNRTFDAGAP
                     HCPEFMDKGWLHGPPFVRDGKAYCGYGGAGLVVLDVEDLTRPRCLGELPFTPAFSSRL
                     AGARTHTALPLPGRDLVVVQNEGERFQFFKPDNITDVQAMNNIHMVDVSDPTKPTLIA
                     QFPYPEVPKDFPYPNFNVAGLGKPGPFGPHNLHEPMDNKPWLEQRGDRVYCCYFHAGL
                     RVYDVSDPYYIKELAYFIPPNPNKTPEESYFPGFPGPRLAVTEDLIVDDRGYIIIDAL
                     DDGFYILKMKED"
     gene            54349..55302
                     /locus_tag="ACOIAF_000466"
     CDS             54349..55302
                     /locus_tag="ACOIAF_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HGZ2667497.1"
                     /translation="MKILGISFGTKNGNNDTMCKVALKGAKEAGAEVEFIQMSSINIK
                     HCTGCCACVKTLLSGKGSMCVLKDDFEWLLDKMKDADGIVVSDPIFEEGASGLFHTIM
                     DRFGPRADRGNNIIGTKVAEVIGGKIPDSRMLNDKVISFMGIGGSDWGTRVQCEHAML
                     ALIPMWKVIDNAWFPWAKELVMDDTRLVQVHQIGLNIVEAAKDFEKASYQGEEGVCPH
                     CHNRLFYLEPGTKKAICALCGIVGELDTISGKTIFSFPEEQLGHAHDTLPGKFIHGDD
                     IKKMEGHYAEVRKTQEFKERKTSYNFIEPLTKEKQAGVSLG"
     gene            55362..55586
                     /locus_tag="ACOIAF_000467"
     CDS             55362..55586
                     /locus_tag="ACOIAF_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667498.1"
                     /translation="MNEKPSFKQIEVILAVIFLVLTYYKVRLTPAFIVIFIPLYLSYF
                     AVSMIVNQIAIKLLEIRQAREATNYNIGNG"
     gene            55579..56091
                     /locus_tag="ACOIAF_000468"
     CDS             55579..56091
                     /locus_tag="ACOIAF_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HGZ2667499.1"
                     /translation="MDRKKKEILTLSMGIALLPPLWAVLAPYIGIKTGAVALICAGLY
                     VTNGNKQKDGLKIMFGFWCGDLWAVLAILIMGYMNFNQNLELFLTLSILGFFAVVIAS
                     LFEKIIFLPSWLCGWAIGLTVMTGENIINLQDICIQIAVAMAVGVWYVGAGVDLFTIY
                     ILSKDRKKGN"
     gene            56123..56977
                     /locus_tag="ACOIAF_000469"
     CDS             56123..56977
                     /locus_tag="ACOIAF_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase"
                     /protein_id="HGZ2667500.1"
                     /translation="MAMGFEFKIMRSLIYVGLAKEEYRPKLMDWLYRHHIPDSISTFG
                     PYCTKYAFYQAYPTPNEGERFGARKMQLTEHYWLVDEHMPEMANRIMTEYMPMDVLRW
                     QGCIPDVENKRVHENVESGDAGRAVGGDNGCPPFIFAFVPINWEEDFRGKGRTVQDGP
                     NYRWQFMIKYPDGISKEEGEKWFYDEVVPYFTNCCYVNRFVSSKIMINYGATAFDRVS
                     ELWFEGEEEWYKAVVEETKSFIKKPEWAQEEEFPYLKPQFNIASVFLGDIATMDAYSQ
                     YRGYIPMR"
     gene            57511..58305
                     /locus_tag="ACOIAF_000470"
     CDS             57511..58305
                     /locus_tag="ACOIAF_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HGZ2667501.1"
                     /translation="MYTIGQVSKFLGISRDTLKFYEDKGLVNPKKNDENGYRIYNQVD
                     IYDIATINFYREIDIEIKKIQEIRKSKSINNLELLLEEKEQIILKEIEYKKLLLKKVQ
                     LVKEDCDKIKTYFGKYTIKEMKPLEVKGKIKHVTNYEEYEALKENTNNLKKAVTLTDL
                     RRVINFNEEGIIEEKFIIVREVDDYNKIIEGEVLSHPNCIYTIIEDGRWSTGGENIDN
                     KVEDRLREIAIENECELLGIVYVNILLTTYEEGIERIFLEIYAPIK"
     gene            58409..59233
                     /locus_tag="ACOIAF_000471"
     CDS             58409..59233
                     /locus_tag="ACOIAF_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HGZ2667502.1"
                     /translation="MRTEKSLKRPIASFIILTNIIFLPLFLLVVVTIMLKLPTVISDI
                     ALCISAWSSTFAFMILFKKIYPDKKFLVHVKDRFRNKLKFSTVSIIISIQVLIAVVVI
                     FILASKNHGIMPNFTVSSWGAFIYLFLKNLFAGSLGEELGWRGFVQNHLQKRHSPLKA
                     SIIVGFWWGMWHLPIWFTTGFKGLDLIKYIIFFMIAIISISIIMTTFYNLNKTLIIPI
                     VIHQLFNFLIGIINGDLIELINYYAMLYLIVAIVLVAINPGNALYKRFSKNTTQIL"
BASE COUNT        22543 a         7212 c         9855 g        19754 t
ORIGIN      
        1 gttcgtgttt atttcttgtt ccttttttca aggacaagta atactatacc atctttaaaa
       61 cagtaggtca atactttttt tatatttttt tataaatttt tttcatattc ttttttcagc
      121 ttctctacta cctttttctc tagtctagaa acagtcatct gtgatatccc tagttcttta
      181 gctatactag attgtgtttt atcaaagaaa aatctatctt taaaaatttt aacttctaat
      241 tcatttaaag tttcaataaa cttattaata aaatcttcat actctatact tccaaagtta
      301 ccttcttctt ttccaatctt atcaataaga gttatatctt tatcctctga atcatcatta
      361 cttgatgaat ccaaagacat tggttgatat ccataagaag cctccatagc ctctaaaaca
      421 tcttcttcac taacgcctat ataatcagct atatctttta cctttggatg cttcttattt
      481 tcttgttcta atttaatctt cgcatcactt atctttttgc ttaattcctg tattcttctt
      541 gggactctaa gtgtccatac cttatctcta aaatactttt ttatttctcc tactatagtt
      601 ggtgttgcaa agctggagaa ctcaaatcct ttctcaacat catatctatc aatagcataa
      661 attaatgcca atgaagcaac ttgatatata tcttcaaaat caacaccttt gtttatatac
      721 ttcttagcta acaatcttgc taaatacaga tgtctttcaa taagaatatt tctaatgtct
      781 acatttttat ttttattata taaattaaat agctcttttg tatccatatt taggtaatgt
      841 gtagcattag ctacattttt cattaaatat caactcctaa atatttagtc atttttattt
      901 ttgtcccttg attatgttct gattcaatat ctacatcatc cattaaagtt ttaattataa
      961 acaatccaag accactctct tttggattct ctaaatctgg tgtacttatt gagctaacat
     1021 catatccttt tccattatct tgaatttcta tattaattgc attatctaaa atattaaata
     1081 ctatattaaa cacatcatct ttactgtgtt ttattgcatt tgtacatgct tcagatactg
     1141 ctacctttat atcttctata tcatctattg gaaaacccat cttattggca attccagatg
     1201 ctgttaatct tattatacta acataatctg gatttgtagt aatttccatc tttatagtct
     1261 cacaagccaa agtttatccc tccactttaa atattttatc tagacctgtt atagtaaaaa
     1321 tctttcttac gttactctta ggatttacta tgtatatctc tttttcattt atttttaatt
     1381 tttttaatac acctatcata gctcctaagc ctgttgagtc tatataatct aaatctttaa
     1441 gattaatctt tacatctaac atattttttt ctattaaagc atgtaaatgt tcctttagtt
     1501 tatctgctgt agaaacatca agttctccat caagacaaac attccaaaat ttattttgtg
     1561 aatctaaatt tgaatcaata ttcatagaca tgtaaaaacc tcctatttcc ttttaataat
     1621 tttaaatata tttcctatac tcaatatttt attaattaca taaactatat catctattcc
     1681 ctttgatatt gatgatgcat tttcaacaat atcttgtata tgtctttcat tataatctac
     1741 aatcttatat gccttttcaa caaccttatt tatgctgttt agagttttag ctaaataaat
     1801 tgctactata agagcagagc ttcctactaa cactgctcca atttgccacc cccatgcatt
     1861 cattagttaa catctccctc atcatcaaag cttttactta ttactatatc ttcttctgaa
     1921 atctttatat cttcttcatt attatcatca tccactaaat ttattattct ctctttaaca
     1981 tcattaaatg tttcatgtag aacttctttt ggattttctt taacatcatt aaacttctct
     2041 tttgtttctt ttcttagttc agaacctttt ttaggtgcaa aaaacatacc aaatataaac
     2101 cctaaaatag ctcctaataa taaaaatcca ccttttttac aacttctttt attgcacata
     2161 acaattactc ctttcaattt taaattttta tctaactaca ttatacaaaa agaataggat
     2221 atttatccta ttcttcttca atatttattt tggcaatcga aactacattg acttcatcat
     2281 tcgtcttcat taatttaaca ccacttgtaa ctcttccaaa cagtgatatt tcattgactc
     2341 ttattctaat aagaactcca tcagaattta taatcattat ctcatcatct tcgttgacca
     2401 tatctgcacc tacaattgta ccagtttttt cagatatatt ataagtttta attcctttac
     2461 cagctcttat ttgacttcta tactcttcta tatttgttct ctttccaaaa ccattcttac
     2521 ttacaactaa cacatctgta cctttactgc aaaggttcat agacacaaca aaatcttctt
     2581 tacttaaagt tatacctttt acacccattg ctgttctacc catatatctt atatcatttt
     2641 catcaaatct tatagacata ccttctttag ttactaaaag tacttcttgt tttccatctg
     2701 taagttctac tccaataagc tcatcatcat ctcttaaacc tattgcaata agaccagatt
     2761 tatttatatt tttgaactct tctctcttag tctttttaac aatacctttt tttgttgcaa
     2821 gtaataaata ttcattttca tcattaccat caataggtat tagagtagca attttctcat
     2881 ctgctgacaa ttgaagtaaa ttcactatag cagtaccttt agcctgtctc ttaccttctg
     2941 gtatttcata tgcatttaat ttaaatactc tacctttatt tgtaaagaat aatagtctac
     3001 tatgagtagt tgtagttacc aagtgcctta caaaatcttc ttctctagtt gtaagtgctg
     3061 aaatacctct tccacccctt ttttgactct tataagtatc agatggaagc ctttttatat
     3121 atccaaagtg agtaagagtt attgctattt cttcatcact tataagatct ctcatatcta
     3181 tttcgccttc agcatgtctt atttctgttc ttctctcatc agagtaattt tcttttatta
     3241 ttgtaatttc atcctttatt acatttaaaa gtaatctttc atcagctaaa atctctttta
     3301 atctatttat cttcttgatt aaatcttcat attcagcttc tatcttatct ctttctaaac
     3361 ctgtaagtct ttgaagtctc atatctaata tagcttgtgc ttggatttca gttaatttga
     3421 atttttctat taaacctagc ttagcttctt gcccagtctt tgaagctctt atcaagctta
     3481 taacagcatc tatattatct aaagcaatct ttaatccttc taaaatatgt gctcttgctt
     3541 cagctttatt tagttcaaat ttagttcttc ttgtaacaac atcttcttga tgtttaatat
     3601 aatgatataa tatttgttta agatttaaaa ctcttggctg accatctaca agtgcaagca
     3661 ttattatact aaaagtatct tccatttgag aatgtttata caaattattt aatactatat
     3721 tagcattagc atccctcttt aattctataa caattctcat accatttcta ttactttcgt
     3781 ctcttaggtc tgatatacct tctattctct tttctttaac taactctgct attctttcaa
     3841 ccagtttagc cttatttact tgataaggta tttctgtaac tattatttgc tgttttcctt
     3901 ttggtagctc ttctataaaa gctctagacc taactttaac ttttcctctt ccagttctgt
     3961 atgcttctgc tatactttct tttcccatta taattgcagc tgtagggaaa tctggtcctt
     4021 gaacaaattt tattaaatca tctacactac actctggatt atctatcaaa tatacagttg
     4081 catcaattac ttctgctaaa ttatgtggag gtattgaagt tgccatacca acagctatac
     4141 catttgagcc atttactaat aaattaggat atctagctgg caatactgaa ggctctttta
     4201 acgactcatc aaagtttggt ttaaagtcta cagtttcctt ttcaatatct ctcagtagtt
     4261 ctaatgataa tttactcatt ttagcttctg tataacgcat agcagctggt gaatccccat
     4321 caacagaacc aaagttacca tgaccatcta ctaaaagtgc tctagttgaa aaatcttgtg
     4381 ccattcttac catagcataa taaacagcag tatctccatg aggatggtac ttacctaaaa
     4441 cgtccccaac aatacgagct gacttcctgt atggtttatc tggagttaaa tttaactcac
     4501 tcattgaata taatattctt ctatgaactg gctttaaacc atctctaaca tcaggaagag
     4561 cacgtccagc tataacactc attgaataat caatatacga ttttttcatt tcttccgcta
     4621 tttcaatagg gagtattttg ttattttctt ccatgtatac taatcccctc tctctatata
     4681 tctaagtttc taacaaatct tgcattttct tctataaatt cttttcttgg agcaacctta
     4741 tcacccataa gcattgaaaa tacttcatct gcaattgcag catcttctac agtaacttgt
     4801 aataaagttc ttgtctcagg attcatagtt gtttcccata attgctctgc attcatctct
     4861 ccaagacctt tgtacctttg aagttcaaca ccatttcttc caatttcatc taataagata
     4921 tttaactctt tatctgaata aacataatgt tccttctttt gctttgtcac tttatataaa
     4981 ggtggctgtg ctgcataaac atacccctca tctataagag gtctcatata tctaaagaag
     5041 aatgttaata ataaagttct tatatgggct ccgtctacat cagcatcggt cataattata
     5101 attttatgat acctagcctt atctatatca aaatcttctc caataccaca accataagct
     5161 gttatcatat tttttatttc atctgaagat agtatcctat ctagtcttga tttctcaaca
     5221 ttaagtattt tccctcttaa tggaagtata gcttgactat ttctatctct accttgttta
     5281 gctgaacctc ccgctgaatc cccttcgact aagaatattt cacttttaga tggatctttt
     5341 tctgcacaat ctgcaagttt tccaggtaaa gatgtacttt ctaatacact ttttctcctt
     5401 gttaattctc ttgctttttt tgcagcttct ctagctcttt gtgctcttaa agctttatca
     5461 actatgattc ttgctgttga tgggttttct tctaggaaag accccagttc atcaactgtt
     5521 acactatcaa ctatacctct cataaagcta ttacctagct ttgttttagt ttgaccttca
     5581 aattgaggtt ctggtaattt aactgatact actgctgtaa gaccttctct tatgtcttca
     5641 ccaagtaggt taacatcatt ttcctttaaa aatttatttc tcttagcata atcatttaca
     5701 gttttagtaa gagctgcttt aaatccactt aaatgagaac caccttcgtg tgtatttata
     5761 ttgtttgcaa aagaatatat gttctctgta taaccatctg tatattgcat agctagttca
     5821 acaaaacaat catcaacctt tttatctata taaactatgt catcatgtat accagttcta
     5881 gatttattta aatacttaat atattctacc aaaccacctg tatagtgaaa ttcttttctt
     5941 ttttcttgtc cttctctttt atcttcaaat actattttta ttcctttatt caaaaaagaa
     6001 agttctctaa gcctatactc caatgtttca tatttaaact ctatttcatc aaatattgtc
     6061 tcatctggca taaattgaat cattgttcct ttatcttgag attctccaac tacttcaagc
     6121 ttagatgttg gcaatccttt ctcatatgtc tgcttatata tctttccgtt taaataaacc
     6181 tctgctacca tccattttga caatgcatta actacagaaa caccaactcc atgaagacct
     6241 ccagatactt tatatcctcc gcctccaaac tttcctcctg catgaagatt agttaatact
     6301 gtttctaaag ttgatttacc agttttagga tgtatttcaa caggtatacc tcttccgtta
     6361 tcttttacta aaacactgcc atcttcatta atagatacat atatgtctga acaatatcct
     6421 tgtaaagcct catctatact attatctaca acttcataaa ctaagtgatg taaacctcta
     6481 gggcttgtac taccaatata catacctggt ctttttctaa cggcttctaa tccctctaat
     6541 acttgtatct gacttgcacc gtattcttgt ttcatttaat ttcctccatt ctctatgcta
     6601 ataacatcgc cattttcaat attaaatatt gtaacatttt tttcatcaaa tattttttta
     6661 tgtgaaactt cagcacttgt tataaacatt tgaacattgc ttaaattatt tactaaaagt
     6721 ttttgtcttg cttcatctaa ctcactaaaa acatcatcta aaattaatac tggatattct
     6781 tcaacttcat tttttattaa ttctatctct gataacttta atgatattga agcagttctt
     6841 tgttgacctt gtgaaccaaa aagtcttgca tctaaatcat ttatgaatat atttaagtca
     6901 tctttgtgta taccatatct ggtagtctta gattctatat cattaggtct attcgatgac
     6961 aacttggcta aaaatttatt atatactgta tctatagtat cttcatcagt tatatttatt
     7021 tgatttttat atcgaattga taatctttca actccattag tcaaattcat atgcatattt
     7081 tcagatatat tagctatctt ttttataaag tctctacgca aaatataaat ataacttcca
     7141 tactttgcta acgtatcatc atatacatct aataaagctt catcaacatg tatattttta
     7201 agcaccctac tcctttgaga aagtgtttta ttataattgg taagatattt ataatattta
     7261 ggtataatct gactaatctc tttgtctata aatgcccttc tttctttagg accctcttta
     7321 accaacctta aatcctcagg agaaaatata acaacattta agttgccaag tagttcttgt
     7381 atcttttgta aaggaacttt atttattctt atcccttttt tatcttttcc aataattagc
     7441 tctattagac tatatttatt atattttgaa aaacttcctc caatatataa gttttcacta
     7501 ttaaatctaa ccatttcttt atctttgtta gtcctaaatg attttccaaa agaaagcata
     7561 taaatagatt ctactatatt tgtctttcct tgaccatttt taccaacaag tagatttact
     7621 ttcccattaa attccagatg caacttttta taatttctaa aattaacaag ttgcaaactt
     7681 ttaagtttca caaaaagttc tccttttttt acaatacttt tattttagtt ccttctattt
     7741 caactatatc tcctgatttt atcttttttc ctcttcttaa ttctatttca tcatttactg
     7801 tcactaaacc ttcttgaatt aaaaattttg catgacctcc tgttgatgca atctctgcta
     7861 atttaagaaa ttgatccaac tttatatatt ctgattctat agttatttca gtcataatag
     7921 ttcctccttg tcactatttt aaacaaattc ctattgatac actaaaagta tattatacca
     7981 taaatatgct tataaaagga gttgtctaag attagaaaaa ttttctacaa aagacaactc
     8041 cttcatgtct gctatatatt tgatgatatt cttactggaa gcagtaagta aatataatta
     8101 acatcatctg ttggtttaat tatacaagga tttacatttg tagtaaattc tataaatatt
     8161 tcctcattat ctatattttt caaaccttcg ataaaatatc tagaattgaa agctatatct
     8221 aggtaatctc catctaaatc tatttcaact tcttcataca catttccttt ttctgtatta
     8281 gaagtaattg ccattacttt atctcttata gataacttta taagattatt tttttctgat
     8341 tgagacaata aagacgctct ctctatacta tttaaaagtt ccttagtatt taatttaact
     8401 ctgctgttat gttctcttgg caataatttt ttataatcaa taaaatctcc ttcaagcaat
     8461 cttgtaataa ttttcgtatc attaattata aaaatagcat ttttttcgtt aaatccaact
     8521 ttaacattat cctctccaga aagcaaacta tttacatcta taagtgtctt tccaggaatt
     8581 atgactttta tattttcagt taaactatca accgaacaac tttttacagc taatctatat
     8641 ccatcaattg ctactaaatt taagtttcta tctacaattt ctaaaagttc tcccattaaa
     8701 acaggttttg tttggtcctg tgagatagca aatacagttt gtttaatcat attttttagg
     8761 atttcttgag gtatgctata taaatcttct tcatttaatt ctggtaattt aggaaattct
     8821 tttgctgcat aacctttaat tttaaatctt gaatttacac aatttatgta aatattattt
     8881 tctgaatcag tttctatctc aacaaaggaa tctggtaatt ttctaattat atctccaaat
     8941 agccttgcat ctacaacaac atcacctttt tctatgattt ctgcttgtac ataagtttct
     9001 attcctattt cagcatcata tccagtaagt tttaattgac cttcttctgt agatataagt
     9061 atacctttta ataattctat tgttgttttt ccattaatag ctttttgagc aattccaatt
     9121 ctatttgcta ggattttttg attacaaatt attttcaatg tggataacct cctctggctt
     9181 tttatatata aaaataagtt aaaatcatag tagtaatagt agtaggtgtt gttgataatg
     9241 ttaataagta tgtataacat tataaataca aagatattaa catgtgtata agatgttaat
     9301 atctttgtta ttttagactt gataatttgt taataatttt tgttcaaaaa aagtattaac
     9361 aggttattca agagatataa acatacaatt gtgtataaat tatcccttca aatctgatat
     9421 aattttgtct attttagtct taatttcttc actttcttct atatccttag atactttgtc
     9481 atgagcatga attactgttg tatggtctct acccccaaac tcttcaccta ttttaggaag
     9541 ggataaatca gtaagctcgc gcgttaaata catagctatc tgtcttggat aagctattga
     9601 acgtgttctt tttttagaat taaaatcttc catctttaaa ttgaatacag aagatacttt
     9661 ttcttttatt ctgagaacat ttatttcttc atttttagaa gttgttataa tatcttttaa
     9721 agcttctgta gcaagatcaa aagatattac cctattgcta agagaagaat aagcaaccac
     9781 tctagttaat gcaccttcta gttcccttat gtttgactta atattttttg ctatatatga
     9841 cataacctca tttggcacat ctatacgttc tagttgagcc ttttttctaa gtatagcaat
     9901 tcttgtctca aagtcaggtg cttgaatatc agttataagt cccatttcaa atctcgatct
     9961 tagcctatct tctagtgtag gtatgtcttt tggcggtcta tcactagata taattatttg
    10021 tttgtttgct tcatgaagag tattgaatgt atggaaaaac tcttcttgag ttctttcttt
    10081 tcctgctata aattgaatat cgtcgattag taatacatct acgtttctat atttattcct
    10141 aaattcttcg tttttatcgt cttttataga gtttataagt tcattagtaa atttttctga
    10201 agaaacgtaa actacttttg agtctttttt ttgactaaca atatgatgac ctatagcatg
    10261 cattaggtga gtcttaccta atccaactcc tccatacaaa aaaagtgggt tataagcttt
    10321 agctggagat tctgcaactg caacgcatgc agcatgtgca aatctattgc tattaccaat
    10381 aacgaatgta tcaaatgtgt attttggatt tagattgggg taaagaactc tataatttaa
    10441 atctgtgcta tcagaattta aatcagcaac ctctttttcg ctcaatacaa atttaatatt
    10501 atattttttt aatgacagtt gactaatagc atcttcaatt aaatgtaaat atctattctc
    10561 taagatatct ttattaaaat cactaggagc cagtaagatt aaatcattaa gatgtatttt
    10621 taatggtacg atgtttttaa aaaaggtatt aaaacttact gaagttaagt caccttttat
    10681 taattgtagg gttttgtccc ataaagaaac tatatccata atataaacct ccgaacagaa
    10741 taattaatat tttccacaaa gttattaaca gaaaagcaaa aatatatata aattttacag
    10801 aataactaaa tttatggata atggtgttga taaattaata atagtataaa ttattaaaaa
    10861 aatatgaaaa gatataaaca atatgtagat aaagttataa ataacataaa catacaataa
    10921 atggtgatta taactagagt attgtcagta atcttaaaag ttatacacaa aagtatccac
    10981 aagctgtgaa taatttctaa taagttgata acatttatgc acattatcaa caagtatatt
    11041 tataatatca aaatatatgt caaacttcaa tgatttgtaa ctaatttatc cacaatatta
    11101 actaattgtt gataaaaata taaacatgtt aaaattactt tatttgtgta tataattgaa
    11161 aaaaataatt tagaataaaa ttaaatagat ttttgctagt attttcaata aaaaaaatac
    11221 ataatatata tatggtagat tttgtgatat aagcgtccca aaaactcgtt gacaacagta
    11281 gtaaaagtat ttataattat atgtgttatg tttactttga gggtaaagtt attattttta
    11341 taaacggaaa ataaaatctg aaaggcggtg caaaataatg agtaaaagaa cttatcaacc
    11401 aaaaaagaga caaagaagta aagaacatgg tttcagaaaa agaatgaaga cgtctaacgg
    11461 aagaaacgtg ttaaagagaa gaagagctaa aggaagaaat agattaactc attaattaca
    11521 aaagggccgc tgcaaaggtg gcctttttgt gtaaataatt acaattcata gctgtgttta
    11581 tggaggttaa ctatggactt taataggact aaagggttga aaaaagactc tgattttaga
    11641 aaagtatata aacacggcaa atcttttgca aacaaatatt tagtaatata tatacttaaa
    11701 aataaatcag attatagtag agtaggtatt tctgtttcaa aaaaagtagg aaaagctatt
    11761 actaggaata gagtaagaag attaataaaa gaagcctaca gattaaatat tgatgaaaaa
    11821 attaagcctg gatatgatat agtatttata gcaagggtat ctagtaagga tgctactttt
    11881 aaagacatag acaagtctat aaaaaattta gttaaaagaa cggatatttc tatttaattg
    11941 tatgctataa atatggaggt taatgacttt gaataaaata atttatatat taaaggaaat
    12001 aagtaagtat ttatcgaatt tatgtattta tttagtgaga ttttatcaaa agtatatatc
    12061 tcccttaaag ggacctacct gtagatttta tccaacttgc tcacaatatt caatagaggc
    12121 gtttaagaaa tatggatttg taaaaggtat gtatttaacg ataagaagaa ttttaaaatg
    12181 tcatcctttt catccaggag gttacgaccc tctaaaataa aagagagtaa ctattaggag
    12241 gtataaaaaa attgaatata ataggaaatt tcttaggatt gattcttaag gtaatatttg
    12301 aatttgttaa tcattatggt atttctataa tactttttac gatacttgta aagattattt
    12361 tattaccatt aacaattaag cagactaagt caacaaaggc aatgcaagat atacaacctc
    12421 gtataaaaga gatacaagaa aaatataaaa ataagccaga aaaacaaaat gaagaaattg
    12481 ttaagttata tggagaagct aaaattaatc cactatctgg ttgtttacct ttattaatac
    12541 agtttcctat attaatagga ttatttagcg ttttgagaga accagttgct catggcgttt
    12601 ttgcaaacaa agctgcattt ttagcagctg acaatgggtt tttatggata aaaagcttaa
    12661 catcaccaga ttatgtatta gcagtatttt caggagcaag tgcatatgtc atgcaaaaag
    12721 taatgacacc aaaagatcag ttacaaggct ctatgaaagt aatgacatat gtaatggcag
    12781 gtatgtcttt ctattgggga tttatattcc cagcaggact tacattgtac tggacagtaa
    12841 gtaacttatt ctcaatagct caatattact taataatgaa tcctttaaaa gctaaactgg
    12901 ctgctaactc taaggaggaa aaagttaatg agaaaaagcc taaaattaat aaaaagtaag
    12961 agtaaagagg aagctataag taaagctata gcagagttga atttaaaagc agaggatata
    13021 gaagtagaaa tacttgaaaa tccaagtaaa gggtttttag gttttatagg tgctaaagat
    13081 ggtacttatg aaatttttgt tatcgagagg gaagtagatg tagctaaaaa ttttatagag
    13141 gtaatgttga aaaatgcaaa tgttgatgcc aaagtcaatg tatctcaaaa agataattta
    13201 atcatggttg atattgaagg caaagaagct gcaagtttaa ttggtagaag aggggaaact
    13261 ttagattcta ttcaattttt gactggatta gctcttaata aaataaataa agattctcat
    13321 acaagagtac ttgtagatat agaaaattat agatcaaaac gagaagaatc tttaataaga
    13381 tatgctaata aagttgctag agaagtagcg aaaacaagaa aaactaaaaa attagattat
    13441 atgaatccat atgagagaag aataatacat tcagctcttc aaaatgacaa atatgtaatt
    13501 acatatagtg aaggaaccga tccatataga agattagtaa ttgagtgtaa aagataaagt
    13561 caacctccta aacattaggg ggttttattt tttaatatta ttaaatcatt aatgattggt
    13621 taaatgtaaa tctatatgta aaaaattatg aattaagagg tgataaattt gtttatagat
    13681 gatacaattg cagcaatagc tacagcacct ggagaaggtg gtataggtat acttagaata
    13741 agtggagaaa aagctttgaa ggttgcagaa gaaatcttta aatctatgtc tggaaagagt
    13801 attgaagaat acaataagag aacattaata tatggaaata tagtggataa tgaaaatata
    13861 attgatgaag ttttattggc atatatgaaa ggaccaaatt cctatacagg agaagatgtt
    13921 atagaaatta attgccatgg tggatttatt tcggtaaaga agatattaga attaatacta
    13981 tcaaaagatg ttagattagc agaggctggt gagtttacta aaagagcatt cttaaatgga
    14041 agaatagatt tatctcaagc agaggctgta attgatgtta taaaagccaa aactgatata
    14101 gctcatgaag ttgctcaaaa tcaattagaa ggttctttat caaagaagat aagagaattg
    14161 agagataaag tcactgagat attagctcat gtagaagttg ctatagatta tcctgaggaa
    14221 gatattgagc atataacata tcaaactctt aaagaaaaaa ctgatgaact taaaaaagac
    14281 attaaaaaat tatatgatac agctgaaagt ggaaaaatac ttagagaagg cttaaagacg
    14341 gtaattgttg ggaaaccaaa tgtaggtaaa tcttcactac ttaattctat tttaggagag
    14401 aatagagcaa tagttactga tataccagga actactagag atgttataga agagtttgta
    14461 aacataaaag gtataccttt aaaaattgtt gacacagcag gtataagaga tactgatgat
    14521 attgttgaaa aaataggtgt tgaaaagtct aaagaatcat ttactagtgc agatttgata
    14581 gtaatggttt tagatgcatc tagaaagttg tcagaagaag atatagagat attagaaaaa
    14641 cttaaggata aacaaacaat agtgctgtta aataagaatg atttgaaaca agagattgaa
    14701 gaagaaaaaa tacttaaata tgtagaaaat aatagtataa taaaaatatc agctttacaa
    14761 caagaaggaa tagaagaatt gcaagataaa atagagtcta tggtatataa aggtagtatc
    14821 aaaaataatt ctagtttagt agtaacaaat tcaagacata aagatgcact tagtaaagca
    14881 tataaatctg caacagatgc tttaattgct ttggaacaaa gtatgccatt tgactttgta
    14941 gaagtcgatt taaaaaatat atgggactat cttggatata ttaatggtga tacagtaaca
    15001 gaggatttac tagataatat attccataat ttctgtattg gtaagtaatg tacaaaagta
    15061 aacttgtttt tattatagag attttaaaaa aatttgaagt gtttctcatg aaacattaat
    15121 tttgaaaaga aaggaattat aaatgataaa gtttgaagca ggaaaatatg atgttatagt
    15181 agtcggagca ggacatgctg gatgtgaagc tgctttggct actgcaagaa tgggatataa
    15241 aactttgata ataacaatgt ctttggattc catagcatta atgccttgta atccatcaat
    15301 tggagggact ggtaaaggtc aattagttaa agaaatagat gccttaggcg gtcagatggg
    15361 attaaatata gacaagacat atatacaaag tagaatgtta aatactgcca aaggtccagc
    15421 ggtacactct ttaagagcac aagcagataa atttaaatat catgaagaaa tgaaaaagac
    15481 attagaagat gaaccaaatc ttgatatagc tatggatgaa gttgtagaaa tacttcatga
    15541 aggaaatgtt gtaataggag ttggaactaa actaggatgt tcatttaaat ctaaagctgt
    15601 aatattggcc actggagttt atctgaactc aaagatatat atgggtgaag ttgcatttta
    15661 tgaaggtcct aatgcattgg ggtatgcaaa atatcttaca gatagcttag ttgagcttgg
    15721 ccttagaatg agaagattta agacgggaac tccagcaaga gttcatagag atagtataga
    15781 cttctctgta atgtctttac aagagggcga tgaaaaagta actccattct catttatgaa
    15841 tgaaaatata gaaaaaaaac aagaaccttg ttatttaaca agaactacag aagaaactca
    15901 aaaagttata ctggataatt taaaaaggtc tgctatgtat agtggagtaa tagaaagtac
    15961 tggtccaaga tactgtcctt ctatagaaga taaagtagta agatttagtg ataaaacatc
    16021 acatcaatta tttatagaac cagaagggtt aaatactaaa gaaatgtata tacaaggaat
    16081 ttcaactagt ttaccttttg aagttcaatt agatatgtac aaaactataa aaggacttga
    16141 aaattgtaag ataatgagac ctgcatatgc tattgaatat gattgtgttg acccaactca
    16201 actaaaaata agtttggaaa taaagggtgt tgagaactta tttagtgcag ggcaatttaa
    16261 tggaacttct ggatacgagg aagcagcagc tcaagggctt atggctggta taaatgcagt
    16321 tagaaagata gaaggaaaag agccatttgt attagaccgt tcagaagctt atataggtgt
    16381 cttattagat gaccttgtta ctaaaggaac taacgagcct tacagaatga tgacatcaag
    16441 agcagaatat cgactatatt taagacaaga taatgcagat atgagattaa ctcaaaaagg
    16501 ttatgatata ggattagtta aaaaagatag atatgaaagg tttctaaata aaaaggctgc
    16561 tgtagagaaa gaatttgaaa gactaaaaaa tgaaagagtt acaccaaaag aagtaaatag
    16621 tttgttggaa gaaaaaggag caacaccaat aaaggttgga atatctctat atgaattttt
    16681 aaagagacca gaggttactt acgaattgct tgaagaatta gggaaaggcg ctggagatga
    16741 tgtttcaaga gaagttaaag aacagtgtgt aataataact aaatatgaag gttatataga
    16801 gaaacaattg aaacaaatag accaatttaa aaaacttgag aataaaaagc tggatgagaa
    16861 aataaattat tcatctatag aaggtcttag attagaagct agacagaaat tagatgatat
    16921 aaaacctatt tcaatagggc aagcatctcg tatatctggt gtttctccag ctgatatatc
    16981 agttttactt atatacctag aacaaatcag aagaactaga ggtggcaaag gtgaataata
    17041 tagaacttct gaaaaacggg attgaaggtt ttaatataga tacgaatgac agtatgttag
    17101 aaaaatttaa aatctataga gagatattag ttgaatggaa taaaaagatg aatcttactg
    17161 gaatagaaga tgaaaaagaa gtgtatataa aacatttttt agactctatt tcagctgtaa
    17221 aaaatggata tataaaaaat gggatgtcta taatagatgt tggtacaggt gctggttttc
    17281 ctggaatacc attgaaaata tgtttagata gcttagaact tactttgtta gattcgttaa
    17341 ataaaagaat aaacttttta gaggaagtta gtagagtact agagttagat aatattacat
    17401 ttatacatgg aagagctgaa gattttggta aaaatgaaga ctatagggaa aaatatgatg
    17461 ttgctacggc aagagctgtt gctggattac cgattcttat ggaattttgt gtaccatttg
    17521 taaaagttgg agggtatttt atttgcttaa aaggtccgaa tgctaattta gagctagaag
    17581 aatcacaaaa ggctattgat gttttaggat tggagtatat tgaaaaaata gatgtagagt
    17641 taccagaggt agatttaaat cataatatat tagtttttaa aaaagtgatg gaaacaccag
    17701 ttaagtaccc tagaaaagct ggtaaaccag ccaaaaatcc tattaagtaa tgaaaaattt
    17761 attattggta agcttaatta tttctcagga aataattaag cttactgtaa aaaaacaaaa
    17821 ataaaggaat tattaaagta gatgcagaaa taaatttgta tgtacatata gagaacaaaa
    17881 ataatttata tctaaaataa gagaggtatg ttatggaaga taaaagagtt atggagatac
    17941 ctatagaaga tgtagttcca aatccatatc aaccaagaaa aatattttca caagtttcat
    18001 tggaggaatt gagcaattct ataaaggtat atggtataat tcaacctata acagttagag
    18061 caaaagatgg caaatatgaa ctaattgcag gagaaagaag attaagagca gctaaattag
    18121 cagagcttaa aaccatacca gcaattataa ataatatgaa cgatgaatct tctgctgttc
    18181 tagcgttatt agaaaatttg caacgagaag acttaaattt cattgaagaa gcaattggtt
    18241 atgaaaactt aataaaagaa catgctttta ctcaacagca attagcagaa aaattgggca
    18301 aaaatcaatc tactgtagca aataagctta gaatattaaa gcttccaaac gatataaaaa
    18361 tgaagctaat agagaataat ttaacagaaa gacatgctag agcatttctt aaactaccaa
    18421 gtgaagattt aatgcaaagt gtgttagaca aagtcataaa aaatgaattg actgttaaaa
    18481 aaacagaaaa attgatacaa gatgtattag aagaaactaa agttcaagaa gagcctgata
    18541 aaaaacagaa tataaagggt gctatgagta taagaattta cataaatact ataaagcagg
    18601 cctttgatgc tatatcaaat acaggaattg atgctaagta taatgaaata gataagggag
    18661 attatatgga agttgttgta aaaatcccta aaaaatagag attactgctt accagtaatc
    18721 tttttttgta atataagcct tgtaatataa attgatttat agacattatt tatacttaaa
    18781 aaacacctat tcaacattta aaattaattt aattcttaaa ggggggaaca tcatgggcaa
    18841 agttatagct gtattcaatc aaaagggtgg agtcgggaaa actacaacaa atgtaaattt
    18901 aagtgctagc ttaggaactt tgggaaagaa aatattagta ctagatttag acccacaagg
    18961 aaatacaact agtggatatg gtattaataa aaacgaagta gaaaatacaa tctatgaaat
    19021 tatgttagat gggttacata ttaaagaagc aataataagt actgaatttg agaatgtaga
    19081 tgtagttcca tcagctactg aactatcagg tgcagagata gagcttacat ctaaaactaa
    19141 tagagagtat attttaaaaa attccataaa agcggtaata gatgaatatg attatatatt
    19201 tttagattgt ccaccctcac tggggatgct tacaataaat tgtttaactg ctgtagatag
    19261 tgttttaatt ccaatacaat gtgaatatta tgctttagaa ggtgttagtc aattaatgga
    19321 gactataaaa ttagtaaaat caagattaaa tgctgatata gaaattcaag gtgttgtttt
    19381 aagtatgttt gatggaagag ctaatttatc aatacaagta gttgaagaag ttaagaaata
    19441 ctttaaaggt agtgtatata caactttaat tccaagaaat gtaagacttg cagaagctcc
    19501 aagtcatgga aaacctgtaa tttattatga taaaagatgt agagggtctg tagcttattt
    19561 ggagctagca gaagaattta tagatttaga agaggaggaa tggtagtatg gagaataagt
    19621 ctaaaagaag taatagatta ggtagaggtc ttagtgcatt gataccagaa attaaaggag
    19681 aaacttcaga aaaagaaata gtcaacatag atatagacaa aatatatcca aacgaagttc
    19741 aacctagaaa gcaatttgat gaagaaaaga taaaagtatt gtcggattct ataaaaaatt
    19801 atggtgtttt acagccaata gtggttaaaa tggatgaaaa taataaatat atgataatag
    19861 ctggagaaag aagatttaga gcatcaaaac ttgcaaataa aaatcaaata cctgctataa
    19921 ttaaagatat agatatgaaa gatataatgg agatagcctt aattgaaaac ttacagaggg
    19981 aagatttaaa ttcaatagaa gaggctctag catataagag tttaatagag cattacaatg
    20041 taactcagga agaaatatca gaagctgttg ggaagagtag acctcatata actaatacac
    20101 taagactttt aaatcttgga caagatgtta tagaaatgat agacagtggt agaattacag
    20161 caggccatgg taaggcttta ttgagaattg cagataaaga tttgcaactt caaatagcga
    20221 aaaaaataga agaagaagaa ctttctgtaa gagaagtcga aaatatagct aaaaaaatat
    20281 ctgaaaataa acaagaagaa cctaaaaaat ctaaacctaa ggatgtattt attttagatg
    20341 ttgaagataa acttagaaat atatttggaa caaaggtgaa tatttctaaa ggaaagaaaa
    20401 aaggtaaaat agagatagaa tactataatg atgatgattt aaatagtatt gtatcaatgc
    20461 ttcttgaata gaacaaaaat ataaaacaga gaggaagatt gttatgattt atttagataa
    20521 tgctgcaact acatatccaa aaccagaaag agtgtataat gctgttttag attgtatgaa
    20581 aaattattgt gcaaatccag gtagagcagg ccataaactt gctatgagag ctgctaggga
    20641 gatatatgat acaagagaga atatagctaa gttgttcaat gtaagtaatc ctatgaatat
    20701 agtatttaca tctaatgcaa cagattcttt aaatttagcg ataaaaggtg tattacaaga
    20761 aggagaccat gtaataacaa cgtctatgga gcataattca gtaataagac ctataaaagc
    20821 attggaaaaa aggggtattg aaaatactgt agtaaaatgc gattatgagg gatttttaga
    20881 ttatgaagat ttagaaaaat caataaagag taatactaaa ctgattgtaa caacacatgc
    20941 gtctaatgta tgtggaactt taatagatat aaaaaaagtt ggagaaatag ctaaaaaaca
    21001 taatatacta tttttagtag atgcatcaca aacagcaggt gtttatgata tagacgtaaa
    21061 tgaatgtaat atagatatgt tggcaatgcc aggtcataaa tgcttatttg gacctcaagg
    21121 aacaggtata ttatatgtaa gagaagggtt gaacttaaat atattaaaag aaggtggaac
    21181 aggtagtaaa tcagaagaaa tagttcaacc agagttattc ccagataaat atgaatcagg
    21241 aactcataat actccaggta tagcaggtct taaccaaggt atactattta tatttgaaag
    21301 aggaatcaac aatataagac aacatgaaga agaattatgt caatatatga tagacaaatt
    21361 agaggaagta ccagatataa agatatatgg accaaaggac agtaaaaaaa gagcatcagt
    21421 aatagctcta aatataggag atatggattc tggagaggtt actttcttat tagatagtga
    21481 ttataatata gcgactagat caggaataca ttgttcacca ctagcacata ccactttagg
    21541 tactttaaaa caaggtgcag taaggtttag tataggatat ttcaacacaa aagatgagat
    21601 agataaagca gtggaagctt tgaaaaaaat ttcaaaaaat aaatagcatg agatttaata
    21661 atatagaaat aaaaaaaggg gatatttcaa aataaaaatt tgaatcatct ccttttttgt
    21721 acaaaaaaga aaaggggtcc ctaagaacct ctaatctatt tgataattgt tttaagaagt
    21781 tactttaagt aatctccttc ttcaagaact atatgaccac tttcgtaatt agaaccatca
    21841 acagtaattt ttatttgttc aacattcata ttttttatgt aagtacgagc aactgaatca
    21901 agcattaaag tttctgcatc actacctaag tttgactttg taaagtcaga gctaacatct
    21961 aatataccag tttttacacc atcaatgtct tttgtagtaa atgaatttaa ttttgcagat
    22021 tctggaacaa ctttaagttt ttgaagttct tcaaataaag tgttctcatc aattttattt
    22081 aaatcaactt tattttcaat taaatctgtc ttatctacat caaaagagta aatagtagct
    22141 gattgagttt tagtttcttc ttttggagta ggtttagctt tgttattagg aacctctttt
    22201 tcagtattgt tttcttcttt tggttgttca acatttgtat tgctcttagt ttctttttta
    22261 gatgaatcac ttccattttg agcattagta caaccaactg cgaatataga tatagataat
    22321 atacttaaca ctaacattat tttcttattt ttcataaaaa tttctcctct cttaaaaact
    22381 attcttatat ataattatag tcgttatgag caaatatatg aatacaaaat agtaacaaat
    22441 ttaaaaaatg atttatttaa attaataaaa ctcaaaataa actaaatttt agtcattttt
    22501 atgggaaatc atttaatgta cagtgaaaat attgtaaaat aaaagtagtg tttttaaaat
    22561 aaatataagt tataattata ttataaaaaa atgtcttcaa aaggtgggaa tagtatggaa
    22621 attttgagct taggagaaaa gattaaaaaa ttaagaaaag aaaaaaacat gacattaaaa
    22681 gaattagctg gagataggat aacagcagca caaataagcc atatagagag ggataaatct
    22741 catactagtt atgaactgtt ggaatatcta ggtgataagc tagatgtaag tgtagaatac
    22801 ttattagaga ccaaggaaat gcaatcaaaa aagataactg ataatttaat actacagagc
    22861 gaaatttata taaaagaaaa tgaattaaac aaggctgagg accaaataaa agaagtttta
    22921 gatatatgta agagatacga tttaatggag aactatggca gatgtaattt tttaatggct
    22981 aatattaatt tgaaaaaatc taattataat gatgctatag aaaactttga aaaggcattg
    23041 tacttcttta taaaaaatag tgatagtgaa aatatactta agtgttattt aaacataggg
    23101 aaaatatata tgcaggaaga cttttataaa ggtgctataa gccactttaa ttttgcagag
    23161 gaaattttgt cagagaataa atttgaggat gttaacatac acaaggaact atatagtaag
    23221 atggcatatt gttacataaa actagataat ccaaatatgt cattgaagta catagaaaag
    23281 ataaatgaaa tagataatag aaatgataga aaagaagatg tcgatatttt ggtgttaaaa
    23341 gcaaataata tgcttgagat tggaaaatat gaagaatcaa gagattactt taaaaaagca
    23401 ctaaaaatac ttgagagtga ggataataaa actggattag caaatgtgta tttaacaatt
    23461 tgtgatgttt acagaaaaat aggtgaaaca gatagagtac ttgaatattc acaaaaggta
    23521 tatgacataa agaaaaatga tgaagatgaa tacatgatgt caggattatt taagataata
    23581 gaagcatata tagataaaga agattatgat ttagctagaa aatattgtaa gatagcctta
    23641 gcttcatcaa taaaaaataa aaataaattc aatgaatata aagcattgag attctatgct
    23701 aatatgtata aaaatcagaa tgaagtaact cttgcaattg aatatctgat taaatgcata
    23761 aaaatagtat cagatttagg caacaataga atattggcca atttatatat agacttaggt
    23821 caattatatt caaatatatc aaaagaaaaa gagttagaat attatcaaaa aggtgtattt
    23881 atgtacaaaa atttagaaat aatgtagggt aaataaacct ataagggtat agaaacaatc
    23941 tataacaagc aagataatat ctatgataaa atataaattg tatgaatatt tttaaggaga
    24001 tgaatcagac aatgagtata aaaatagatg cgagagggct tgcttgtcct aaaccagtta
    24061 taaatacaaa aagagaattg gataacctag aagaaggtgt tgtagttaca atagttgata
    24121 atgaaacagc taaggagaat atattaaaac ttgctaaatc tttaagttgt gaagcaaata
    24181 ttgttgacga aaaagagaat tttatatcta tagaaattag aaaaggtaaa aatatactag
    24241 ataaacaaaa tgttgtagat aataaagaaa gtgaattaga caatacatgt atatttatat
    24301 cttcagataa gatgggactt ggaaatgacg aattaggaaa agtgttaata aaaggattta
    24361 tatatacatt aacagaatct aaaccttatc ctaagtatgt acttttagtt aatggaggag
    24421 taaaattaag tgctgaaaat gaagaaacca tagaaaatct aaaaatactt gagaatatgg
    24481 gtgtagaagt tttatcttgt ggtacttgtt tagattatta taatttaaaa gacaaattac
    24541 aagtaggtac agttacaaat atgtatacta tagttgagac attaaagaat gcatcaaata
    24601 ccatttctat ataatgtatg tatgtttaat aatataaaac taacaataaa gattaactca
    24661 aatagatagt cagaaaggat taagtaatga atcaaatgta tatagtgtca tttaattcga
    24721 cacatcatgc gataagatca gaaaagttat ttggtgaaaa tagtctaaaa gtaatggctc
    24781 ttccaacacc aagagaaata acagcaagtt gtggaatatc aattaaattt tcttttgaag
    24841 atatggaaaa gataaaaact attttggttg aaaataatgt agatataaaa ggaatttatt
    24901 gtataagtaa attagaaaat ggttcaaaag aagtagagaa gttagattag gaggctaatt
    24961 atttatgcca atggatttaa aaataggtga tattgttgaa ttaaaaaagc aacatgcatg
    25021 tgggtgtaaa gagtttgaaa tcataagaac gggaatggat ataaagataa aatgtactaa
    25081 atgttcaagg ctaataatgc ttgatagaga gaccttagaa aagagagtaa aaaagctaat
    25141 aaaaaagtga taaaaattta acacttaaaa aatgtcaaat accgtatatt aatatccaaa
    25201 attttggtat aataagaatt gaagaaaaca ttttcatggg ggtgctttaa ttatggcagt
    25261 taaatatgca aaaagaatgc aaggattaca aggatcagaa atacgtgagc ttttaaaact
    25321 tactcaacaa ccacaaataa tatcttttgc tggtggaatg ccggctccag agttatttcc
    25381 agttgaggaa atgaaaaaag tatcagttgc agtgcttgaa gaaaatggaa gatcagctat
    25441 gcaatatact acaacagaag gatatgaacc attaagagaa aaaatagcag ctagaatgaa
    25501 tgataaaaac aaaactaatg taaacaaaga tgatatatta gttacaagtg gttctcaaca
    25561 aggtctagat tttgcaggaa aagtattcat tgatgagggt gatgtaattt tatgtgaaag
    25621 tccatcttac ataggagcta taaacgcatt taaatcttat caacctaaat tcatagatgt
    25681 tccaacagat tctgatggaa tgataatgga agaattagaa aagatactag aaacaactga
    25741 tagaataaaa atgatttacg taatacctga cttccaaaat ccaacaggaa gaacttggcc
    25801 acttgaaaga cgtaaaaaat tcatggaaat agtaaataaa tttgaaatac cagtaataga
    25861 agataatcca tatggagatt taagatttga aggagagact ttaccatctt taaaatcaat
    25921 ggatacaaaa ggattagtaa tattcttagg aactttctct aaaatattct gcccaggata
    25981 cagattagga tggacttgtg cttctccaga aattttatct aagtttaact ttgcaaaaca
    26041 aggtgcagac ttacaagcat caactatatc tcaaatggaa gtaagcaaat ttatggatat
    26101 gtatgaccta gatgcacatg ttgacaaaat aaaagcagtt tatgttaaac gtagagatgt
    26161 aatgcttaag actatggaag aagagttccc agaaggatta gtatttactc atccagaagg
    26221 tggattattt acatgggtag agcttccaag taatttaaat gctaaagaat taatgccaaa
    26281 atgtttagat aagaatgttg cttatgttgc tggaggagga ttcttcccta atggtggtag
    26341 agaaaatact ttcagactta actattcaaa tatgccagaa gaaaaaataa tagaaggtat
    26401 aaaaaatata gcagctgtat taaaagaagc tatgggtgta gaagcttaat taaaaatata
    26461 acttatatat aatacaaggg atggttgttg ccatcccttg tttgtttgaa aagtatataa
    26521 ttcaccttat actaaacttt gtccaaaatt taaattgaca gaaataagaa taaatgataa
    26581 aatgtgtagg tatgagattt tagtctcatt tctctgctct aagagtgtag agccgttaag
    26641 tccaaaggga ggtgaattat agtgagaaat tatgaattag tttatgtggt aaagccaaac
    26701 tctgatgaag aagtaagaga ggctatacta aacaaagtta aggaagttgt tgcaactgac
    26761 ggagaaatag ttaaagttga tacttgggga actaaaaaat tagcttatcc aatagctaag
    26821 tttacagaag gtttctatgt attagttaac ttcaaatcag cagtagacgt tcctaaagag
    26881 atagacagaa acttaaagat aaatgaaaac gtaataagac acatgatagt tgttgcttaa
    26941 taactggttt tattataaaa aataggtggt gtttttatga atcaagttgt attagtggga
    27001 aggttgacta gagaccctga attaagatac atcccaggta caggaacacc agtggcatcc
    27061 tttacaatag ccattgatag agactatgta aaaaaagatg gatctaaaga aactgatttt
    27121 atacctgttg aagtaatggg taaatctgct gaattttgtg ctaactacat aactaaaggt
    27181 agattagtag cacttcaagg atctataaga gtagacaatt atcagacaca atcaggtgaa
    27241 aagagaactt ttacaaaagt aagtactaga tcagtacaag ctttggatag taagaataaa
    27301 tcggaaaatt catatagaga gagtgctcca gcttttgagc caagctttga acctcaaggt
    27361 ttagacccac aaggtttcca agctatagat gatgacgata taccatttta aaaaggaggg
    27421 aaaaaagtca tgatgaataa gaaaagacgt aagaaaaaaa gagtttgtca attttgtgct
    27481 gacaaaaacg ctaaaataga ttacaaaagt actcaaagat tacaaaagta tataactgaa
    27541 agaggaaaga tattaccaag aagaatatct ggaacttgtg ctaagcatca aagagaatta
    27601 acagttgcta taaaaagagc gagaaatata gcacttttac catatacatt agactaaaaa
    27661 aatgagcctt gtgctcattt tttattatat gtatttatat atctaaatga ggcgagctat
    27721 aaagctcgtt tttttgttta tatacttaaa tatattatta tttatgatat aatattatat
    27781 attaaggagg gttgtaaacg tgaaaataga taaaggttct gagataacga gaaacataaa
    27841 gataatagaa tggatgaaaa cagaaatatt aatgagtgta agtgatttgt ttaatttatt
    27901 gtttaaaggg gtaaaaccat tagatgaagc aattcaagat actttagcaa atataataat
    27961 gataacatat ctcttagcaa aaagattagg aattagtttt agagatgtgg attataaagt
    28021 caaggaaaaa ataaaaattg gtatagatga ggaccatagc gttgaaagat ggtatggaga
    28081 tttatctaat ttaaaaaaac atatggataa tagggagtga tattaaattg aataataaaa
    28141 ttagactatc taaggcaatg tctataatag ttttagctat aataatcgct ttagtaatag
    28201 cctatattcc aatgctagga atatttagta tgttagcagc tgttccatat gtaattatag
    28261 gtgctattac tgatagaaag tattctctta tatctatttt ggttacattt tgtgtactga
    28321 ttctatttgc agacatttct tatgcattaa atatatgtat aatgtattct ttaccaggta
    28381 tagtaatagg aaagatgcta aagagaagct ttgaacaaga ggacagtaat aaattcgaac
    28441 cgatatatgg tggaactata atatttgtac tttcaatgct tgtatatttt tttgtattaa
    28501 agacattttt aaatgtgaat ctattagatg aagtttctaa gatgatatca gaaattgtaa
    28561 atattcaaaa aaatagtttt tctgcaactg aacttaaagt gtttgataaa atgaagccag
    28621 atgagattgt aagttatttt acaaatatgc taccaatgat gttattttta caagggttgt
    28681 tatcagcgtt tgtcacatat tatttgagtg tattttttat aaaaaggatt acaaagatga
    28741 atataagatt tcctaagttt gctgattttt atttaccggg aaatgctata ctcacaactt
    28801 tcttacttta tttattagtc ttatttattc aaattattgg ttcaaaatta tatacagaat
    28861 taataatgac taatttacag ctagttttta atttaatgtt tgtaattcaa ggtatagcag
    28921 tttgcattta tttcttaaag aaatggataa gacagggacc aaataaaatt atgttttttt
    28981 cagggataat tttatgttta tttggattta tgggaatatc ttttgttggt atggtagata
    29041 gtataattga ctttagaaag gtaagaagct ataaatccac ttaggaggat agaaatgagt
    29101 aataaacaaa cctttaaatt aaatatgcca gaaataaatt tatatataat tgttattggt
    29161 atttctagta taattttgct ttattacaac ttgtatgtag ggtgtttatt tttctgtatt
    29221 tttgtatata tggtttttca caattggagg actactaata ttagacgtca tgaatggaca
    29281 gagtatattc aaaatttatc tttagatata gatgaaacaa ctaaaaaagc cataataaat
    29341 cttccaatac cattatgtat tttagaattt gatggaaata tatcctggta taatggaaag
    29401 ttttatgata tgattgatca aaaagattta cttgataaaa atatagagga tatagttaaa
    29461 aatcttaatt taagaaaagt attaaatgaa aataaagaga tgtacacaga aataaactat
    29521 aaagaaaaag aatatacaat aatttataat gttataaaaa atgatcaaga aaaaaatcct
    29581 aaatatttga tgatattata ctggatagac aagactgaat atttaaaagt aaaacaaaat
    29641 tatgatgatg aaaaaaatgc aatgatgcta atacaagtag atggatatga tgaagtatta
    29701 aaaagtgcag cagaagataa gagagcttta attaatgttg aagtagagaa aatactttct
    29761 gctttagaat taaattcaaa tggtgcattg agaagaactt caaaagacaa attcttcttg
    29821 gtaatgcata aaaaagagct aaaaaaattg gaagctgaga agttttctat tttagataca
    29881 ataagacata ttgattatgg aaataatctt cctgttacta taagtatagg aattggtata
    29941 gatggagata cattaaatga gaacctaaaa cttgctacgg gtgctctcga tttagcactt
    30001 ggtagaggtg gtgaccaggc tgttgtaaaa acaaaagata aatttgtgtt ttatggagga
    30061 aaatctaaag ctgtagaaaa gaaaaccaaa gtaaaatcca gattaatagg tcatgcttta
    30121 agagaggtta tacaacaaag tgaccaagtt tatataatgg gacataaata tcctgatatg
    30181 gatgctatgg gagcagcagt tggtgtttat gatatatgta aatcctgtaa caaaactgca
    30241 aacatagttt tacaatctgt aaatgaatct atagaaatat ttataaataa aataaatgaa
    30301 aataattact ataaaaagtt gtttattgga aaagaggaag ctattgacaa ctgcactaaa
    30361 aatacattag ttgtagtagt tgatactcat agaccaaatt atactgaatg tgaagaactg
    30421 ttaaagctat cagagaaggt agtagttata gaccatcata gaagaggtgt agagtttata
    30481 aatgatgcag tgcttttatt ccatgagata tatgtatcct caacgtgtga aatggttaca
    30541 gagttagttc agtatatgga tgaagatgta acaataaata aacttacagc tgaagggctt
    30601 ttagcaggta taagtctaga tactaaaaac tttgcattca aaacaggtgt tagaactttt
    30661 gaagctgctt cttaccttag aaaagttgga gcagatacta tagaggtaaa gaaattcttc
    30721 aattctgatg taaaagattt cataataaaa gctgaaataa ttcaaagtac taaaataatt
    30781 aataatagaa tatgtttagc atattcaagt actgagatag atagtataaa tgttataata
    30841 gctcaaactg ctgatgagtt attaaatatc aaagaagtag aagcttcctt tgtattaggt
    30901 gaaaaagatg atactatatt tataagtgcg aggtctttag gacaaataaa tgtacatgta
    30961 cttatggaaa aattaggagg cgggggacac atagatatag ctggagcaca gttaaaaaat
    31021 gtttctctaa aagaagctta taagatggta aataaaataa tagaggagta tttggaggag
    31081 gaagaataga tgaaagttat attattaaaa gacgtaaaag gaactggtaa aaagggagaa
    31141 atgaaagaag taagtgatgg atatgcaaga aatttcttat ttcctaaaaa gatggctgtg
    31201 caagcagata gtgtagcaat aaaagaatta aatgaaaaga ataaatctaa agaaataaaa
    31261 gcacaaaaag agtatgaaga agcagtttta ttaggtaagc aaatggaaga aattaatata
    31321 gaaatatatt caaaagcagg agaaggtgga agattatttg gttcaataac ttctaaagaa
    31381 attgctgagc aattaaagaa acaaaaagac atagatgtag ataaaagaaa aatattatta
    31441 gatgaaccaa taagatcttt aggttcaact tttgtagaaa taaaaataca tcaaaaagta
    31501 actactaaaa taagagtgga tgtaaaagaa aaacagtaat aactagaggt gattaagatg
    31561 gaagatatga cgagaattcc tcctcatagt gtagaatcag aacaatcaat attgggttct
    31621 atacttctag ataaagatgc tataataacg gttacagaaa ctataaagcc tgatgatttc
    31681 tataaagagg ctcacaaaat aatatatgaa tgtatgataa ctttaagtaa taaaggtgag
    31741 cctatagact tgataacatt aacagaagaa ctaagaaaac aagggcatct taatgatatt
    31801 ggaggaatca gttatattac aagtctttct actatagtac cgacgacttc aaatgtaaaa
    31861 tactatgctg atatagtaaa agaaaagtct gtacttagaa agcttataaa agcttctaat
    31921 gaaataataa atctaggata tagtggagca actaagattg aagatgtttt agaacaagct
    31981 gaaaagagta tatttgatat atcacaggag aaaactagtg atgattttaa atctataaac
    32041 ttagttttga tggatgccta tgatatgata gaaaaattat acactaacaa gagtgatgtc
    32101 actggtataa ctactggttt taaagattta aacaaaaaaa taaatggtct tcaaaggact
    32161 gatttaatat taattgcagc tagaccagca atgggtaaaa ctgccttttc tttaaacttg
    32221 gttcaaaatg cagccttaaa aggtgatgcc tctgtagctg tatttagttt agagatgtct
    32281 aaggaacaat tagtacaacg tatgttatct tcacaatcta gtgtagaatt aaaaaaaata
    32341 aagactggaa cacttaatga taatgattgg cctagaatta tagatgctat ggcagtatta
    32401 tcagatgcca aaatacacat agatgatact cctggtataa aaatatcaga actaaggtca
    32461 aagtgtagaa agttaaaaat agaaaaagga ctagatttag tgcttattga ttatcttcaa
    32521 cttatggaag gtgaaggtaa taatgaaagc agacaacaag agatatctaa gatatcaagg
    32581 tcactaaaaa tattagcaaa agaattaaac tgtcctgtag tagcactatc tcagttatca
    32641 cgtgcaccag aacaaagagc agaccataga cctatgttat ctgatttaag agagtctgga
    32701 gcaatagaac aggatgcaga tatagttatg ttcttataca gggacgaata ttatcatgct
    32761 gattctgaga gtaaaaatat aggagaagta ataatagcaa aaaatagaca cggtgaaaat
    32821 ggttctgtgg aacttgtttg gcttggtgaa gttcaaagat ttggggataa gttgagagac
    32881 ctataatcta acgaatatcc acagaacgtc ctttctttct acaggaaaaa tgtggataca
    32941 tgtcactaaa tatgtccaaa cattgaaatt tagctaaaaa actatccaca ctatccacag
    33001 atggcctgtt gataagtgtg aataagtaga taaagcaagc ttttggcttg ctttatctgt
    33061 gtataaatat taattattag gaggaataat atggaaaaaa taagcttaaa atatatatat
    33121 cccaatataa ttaaagtact tgatgaaata aacttattta gagtaattga caataattta
    33181 agagagtcta ttgttgttta tgctaataat gtggataatc agtatcatat aaacatgact
    33241 aatacaaact ttggaaacat aattaacata tgtaaattag aaaaactttt agatgtggat
    33301 aaatttatgg aaaaagttat aaaatatgaa aaagaaataa tagagaaaga agagttttca
    33361 aaaatagaag aatatatgtt aaatattgga gaatactaaa aaagttgaaa aaaatacgaa
    33421 tgtttgatag aataagtaag taaagtgttc gagagtatat aaaatataga ggtgaattac
    33481 atgaaaacag ttgcaattgt tggttctcaa tggggagatg aaggtaaagg taaagttata
    33541 gattatcttg ctactcaagc agatgtagta gtaagagggc aaggtggaaa taatgcagga
    33601 catacattag ttgtagaagg taaaaaatac gcattacatc taattccatc tggagtttta
    33661 aatccaaata cagtgaatat aatcggaaat ggaatagtgt ttgaccctaa aggattttta
    33721 gaagaattgg aaatgtttaa aacggataat ataagtacag aaaatataaa aataagtgat
    33781 agagcacatg tcatattccc atatcataaa gaactagatg cattgtcaga agaagccaga
    33841 ggcgatttaa agataggtac aacaaaaaaa ggtattggac cttgttatat ggataaaaca
    33901 gagagatctg gtattagaat atgtgactta atggataaag ataaatttgc tataaaatta
    33961 aaagctcaaa tagatgctaa aaatgaaata gttaaaaata tatatggaaa agaagaatta
    34021 tttgattttg aaactatata taacgaatat ctaggatatg cagaacaaat aagaaaatat
    34081 gtggcagata cgtctgttat tgtatatgat gcagtaagag caggtaaaaa agtattgttt
    34141 gaaggagcac aagggacttt attagattta gatttaggaa cttatccatt tgttacatct
    34201 tctcatccaa catctggtgg atttgcaatt ggtgcaggta taggaccaaa tatgataaaa
    34261 gacgttgttg gtatagtaaa agcatatact acaagagttg gagaaggacc gtttgttaca
    34321 gagcaaataa atgaaactgg agataagata agagaacaag gacatgaatt tggggtaact
    34381 actggtagac caagaagatg tgggtggttt gatgcagtta tagttaagta tgcagctaga
    34441 gttaatggat tgactagtat ttcatttatg ttacttgatg tattaactgg atttgataag
    34501 ataaaagttt gtacgtctta taaaatgggc gataagataa taacggattt cccagcttca
    34561 ttagatgatt tagcaaaatg tgaacctgta tatgaagaat tagatggatg gaatgaagat
    34621 ataactcaaa tagataactt tgatgacctt ccagaaaatg caaagaaata tgtagcaaaa
    34681 atagaagaac tagttggagt aagtgtagat atggtttctg ttggtccaaa tagagctcaa
    34741 actataataa gaagaaatat atttgcttaa tattttatca attaaataat ttaaaatagt
    34801 tgaagaagag tatctcaaga taatgtttat agttattaga gatgctcttt tatatttgac
    34861 ttacattgat tttttattaa gttaaagcta cttatacata ttctagctga aattaaagtt
    34921 gaatttatgt agatacatat aggtttttta aaaagaattt tgttgaaaat aaatggctaa
    34981 agatgatttt aattatattc taaaaaaagt aaaaaaaact attgactaaa aattatatag
    35041 acgctataat ataacttgtg agtgaaagaa acgacgaaaa aagtcgaaaa aatgtggtct
    35101 attagctcag tcggtagagc acctgacttt taatcagggt gtcccgcgtt cgagtcgcgg
    35161 atagatcacc aaaatatggc tcgttggtca agaggttaag acaccgccct ttcacggcgg
    35221 taacaggggt tcaattcccc tacgagtcac cagtatggga ctatagctca gctgggagag
    35281 cacctgcctt acaagcaggg ggtcacaggt tcgagccctg ttagtcccac caaattttga
    35341 attagagata aatactagat tatatgctgg tgtggctcaa cggtagagca gctgacttgt
    35401 aatcagcagg ttgtaggttc gattcctatc accagctcca caaagtacat aaattcaaat
    35461 taatataatt ttctatgtta caggatactt ttaagtatct ttttttaatt tcatttttat
    35521 tgtattaaaa atgaaattaa acatacaaca aaattttgaa ttacttgcaa tgtgatagtt
    35581 ttaaatataa aatttagaat cattctagca tagtattatg taatatagca aatttaattt
    35641 atagtatcaa taaatataga ctactaagaa tgtaaatgat ttgttagatt aagatacatt
    35701 agatatgtaa atagaaaaat aaaaagtatg ttttaattta tgtgatataa gttttaaaca
    35761 gagaagcaag tttgctctta cctctctatt taaaaataaa aaaatgtttt ttaagggcta
    35821 ttttatttaa atctttccca tctcaaatca tctccagtaa attctaaaat cctaaactta
    35881 tctaatattc tagagagagt tctttgagtg taagtatttc ctatttgaga tggtgttaag
    35941 ttggttgaaa taatgatttt tttaccagca acaagtcttg tatttacaat attgaagatt
    36001 tctccactag taaaagaatt attaagttca gttccaaggt catctattat taataaatca
    36061 caatcaaaca agtttttata attatcttca cttatttgat tatttgtgtc tcttctaaat
    36121 ttataatctt caagaatatc taaaattcta aaagaagttt gatagattac aacattgcct
    36181 ttatctaaca attcctttgc aatacaattg cacatatagg tctttcctag tccagtagaa
    36241 ccataaaaca gtagattttc actattatcc ttcttaaaat catgaacaaa attttcgcag
    36301 atacttaaat tatttagcat attttctcta ggagatattt caccatcaga gcctttctta
    36361 ggagaaaata tattcaagtt aaaattcgaa aaattttcct gacttaaaat cctatctaaa
    36421 ttagacattt tataagcttc atttacaatt tcttgtttga ggcaagaaca tttttctcca
    36481 ttaggtaaaa atcctttatc tttacaagaa atacattgaa actttatttc caagtaatca
    36541 agaggtatgt tactttcagc taaaagtctt tctttcttta cttttaaaga ctctatattc
    36601 tctttagtct tctttacaat ttcatcttta gacttaggat ttaaaagtac tatcttagct
    36661 aaactgagac ctattttaga aatttcatca tctatagatt ttatctcagg gattctatta
    36721 tatacctcat tttttctatg ttctaataag agttcattat catctcttct cttagcatat
    36781 ttagcaagta tttttcttat tttatcctca ttcatttaat cacctactta aacttttctt
    36841 tttgactttt tttaataatc tcatcgagct catcagaggt atactgagtg aatgtttcgt
    36901 tgaaattatg aaacttagtt tttttgtaag tattgttgtt ttgagtagta ttaatctgct
    36961 tcttattaat attctcctta actatacgtt cttcctcttt ttgctttaaa tcatttaaat
    37021 tttttatatt tttttcattc caatttttaa ttataccatt tatataagat atacttgggt
    37081 ttgaaatatt cttagactta gaacatgcat ttattatcaa atctatatcc atattaaatt
    37141 tatcaaacca ggtgtccata agttcttttt ctgattttga tggttgtctt gaaaatccca
    37201 attcattgaa aatcgtttta tataaaatat atcgttcact tcttaccaaa aagctttctt
    37261 caacatcttt tggcgtatat aaatttgaat cataccaatt tctaataatg ccttcaatgt
    37321 atttaactgg ctttgatgta ccagttttat ctttgacgta ttcatatgcg caaagtatca
    37381 tatcggggct catattgtat ttattcatta tatccataat actcatcttt tcacttgggt
    37441 cgagatatcg cccaactatt ttatttatag aattaaacat ttttctaata cttggatttt
    37501 cagaagtaga tacaacttta tctgtattag attttataga tgaattattg cttaatatat
    37561 tctcaatata gaaatttttt aaatctaaaa attctattga ataattaaaa ttatcatatt
    37621 ctccattgtc atgtattttt ataatcttct tttcttccca gaatttccaa gcagataaca
    37681 catcagacaa tggtatattt aggtttttgg ctattgaatt attgtcgaac ttaggattag
    37741 aagtaatatc acatgcttgt ctatatccta aaagataaac ttttacgtat aacccgtccg
    37801 ccattggcat aaatatatct ataaagatat tgggaatagt gatttcacct aaatctattt
    37861 cattgctttc tttaaaaaac attttatcac ctcagattaa attatatcat tttttatatt
    37921 atatttgtct gtaaacaaat aaaaggtcac aagattagca tgtaaatatt ttctaaaagt
    37981 attcattttg aaaaggagga gatattttgg ttataaaatt caaccaaaaa gtaagaagaa
    38041 cattatcagt agcaattcta tttatggtat taataatagg ttgctttata gaaaaggatg
    38101 acagtttaga aacatacaaa gaaaaaacta attcaaaaat aaatcagtat aatatggatg
    38161 ttatatttga tgatgagact aagaggttaa tgtgcaatca gaatgttgac tacataaata
    38221 atacaaaatc aaacatagat aaaatatatt tccatatata tccgaatgct ttttccaaga
    38281 aagattttgc tccttttgaa aaaagtgaaa tgacaagggc ttatcctaat ggatttaacg
    38341 aaggatatat agatataaag aatatattaa ataataatag taaaatggag tataaaatta
    38401 aaggagacaa aaatgacata ttagaaatag aattagggaa agagttaaaa ccaaatgaaa
    38461 gaatgtcatt agatataaaa tataacgtaa aaataccaaa ttcaattgga agatttggat
    38521 atggagaaaa tactataaat gttactaatt ggtttcctat agcttgtgta aatgatgaaa
    38581 ggggatggaa tttaaagagt tatgaaacta ttggcgaccc attttatagt gagactagcg
    38641 attttcatgt gaaactattg attccaaata aatataaaat tgctcataca ggaaaactta
    38701 ttgatgataa acatgataat aagaaaatgc tatatgaaat agaagctgaa aacgttagag
    38761 attttgcatt tatactaagt agtaaatttg atgtaaacaa agtagattca aagggagtag
    38821 ctatcaatac atataattta aataaaaagc tttctaaaaa agcaactgaa gtggcaaaag
    38881 attcaataaa tatatttagt gaattatttg gaaagtatcc ttacaaagaa ttttctgttg
    38941 tagcaagtga cttttttata ggggggatgg aataccctat gcttgttatg atagaccaaa
    39001 gtttatataa tgaaaaaaat gaatttttac tagaatatgt tattgcccat gagacagcac
    39061 atcaatggtg gtattctgct gttggaaatg atgaaataag tgaaccctgg ttagatgaag
    39121 cacttactga atattctaca atagtttatt ttgaacaaaa gtatggtaaa gatatggcaa
    39181 acaagcttat aaaaactatg gagatacaga caaaaagtta tttgagcgaa aatatattta
    39241 agccagcaaa tgagtataaa aattctactg aatacagctt gaatgtttat acaaaaggcg
    39301 caatagcatt taatgaaata agaaaagaag ttggagataa agtattcttt gagactttaa
    39361 atgagtacta taataagtat aagcataaaa atgctaatgg aagagctttt gtggaactat
    39421 ggaatagtaa aggtgtagat ataaataaga ttataagtga atgtaagtaa atagtgaatg
    39481 taagtaaatt ttgtgcagag tattaaattt tatttaatac tctgtttttt tggtataata
    39541 taatttataa ggcagaaaaa gaggtgaagg aatgctaaag tattgttcag tagggagtgg
    39601 tagtagtggt aattgccact atatagggta taaaaatact aatatactag tagatgcagg
    39661 attaagtggc aagagaataa ctacaggtct taaagacatt ggtgtagatg cagataaatt
    39721 aaaaggaata tttataacac atgagcatgt tgaccatata aaaggagcag gtatattatc
    39781 aagaaagttt gatataccag tatttgctaa tataaaaaca tggtgttcta tgaaggacaa
    39841 attaggtgac atcaaagata aaaatatgaa agtatttgaa aatgataaaa cctactcatt
    39901 aggtgatatc atagtaagac ctttttcaat agaacatgat gcgtctgacc cagttggata
    39961 taatttttat acagagagtg atgaaaaaat gtctatagct actgatatag gatgtataac
    40021 tcaaaatata aaaaaacatc tatataaatc taaattagta gtattggaat caaattatga
    40081 ccctaatatg cttatgatgg gttcatatac atatgcttta aagaaaagag taatgtcaag
    40141 tactggtcat ttatccaatg aagatgcagc taatttctgc gttgaactta ttaatgaagg
    40201 tacagaatct atattattag cacatttaag taaagaaaat aattttcctg agttagcgta
    40261 tgagacttca aaaggagtat tagcatcaaa tgatatagta gttggacaag atgtaaaact
    40321 agatgtacta tcacgaaatg atgtttctaa tgtttatgag atgaagtaga ggtgaataac
    40381 tttgctaaag agaaattttg aaaatgttga ggataataaa attttatact ctataaagca
    40441 gggtatgatt ttagcgatac ctgctattat gactggttca actgctcttg taattttgaa
    40501 tcttccaata aaggcttatc aagaatattt atcaggcttg tttaatggag aggttacaaa
    40561 tattcttaac tttataaatg attctacttt aggaattatt tcattaataa tattattaac
    40621 aataagctat agttatggaa aaatatatgg gagcaagtat acagtacttg ttcctatagt
    40681 tgcaatgtgc tcttttttag tattttcaca tggtaatgag cttaattctt atatagaaat
    40741 atttaaaaca aagtggttat ttacatctat aattgtttct atgacatctt ctgtattgtt
    40801 tgtaaaactt acagaatctt ttgttacaag tgtaaagttt catacagaag gggctgatgc
    40861 agactttaat atggtagttt cggctatagt tcctttcata atagtagtat ttttgttttc
    40921 tatttttaga gtggtaatga taagtctaat tggcagttca aactttcaag atatatttta
    40981 taatttattt tcaaatgttt ttaataaaat gggaacaaac cttatgagtg ctctgttgtt
    41041 tattttttta atgcacttta tgtggttttt tgggattcat ggtagtaatg tattagatac
    41101 tgtcgcaaaa aatttatttg aaaatagtat ggcaattaac ataaatttag ttaataataa
    41161 tcaattacca acagaaattt ttactaagac cttttttgat acaatggttt tattgggagg
    41221 atgtggctct ttactatgtt tagtaattgc aatttttctt agtgaaaaac gtataaatgt
    41281 aagaaagtta gctaaaattg cttcaatacc agctcttttt aatatcaatg agatgctagt
    41341 tttcgggata ccaattgttt ttaactatat tatgtttgta ccttttgtaa ttactcctat
    41401 aatcttaacc atcacaagtt atgtggctat gtctacaggt attgtaccat gtacagtttc
    41461 agctgtagaa tggacctcac ctatattttt aagtggatat atgtctacag gttcaataag
    41521 aggtagtttg ttgcaagtat ttaatttatg tgttggtgtt atgatttata ttccgtttat
    41581 aaaaatgtcg caaaatagat atacatatat gtttaaaaat aatatagaaa atttaacaaa
    41641 cttattaaaa aggtatgaat taacaggtga acagcctaat cttctaaacc ataatggtaa
    41701 aataggttca atttcaaaaa tgttggcatc agacttgaag tttgctatga aaagaggaga
    41761 aatagagctt ttttatcaac cacaagtcaa ttataatgga aatgttgtgg gagcagaggg
    41821 attacttaga tggaaacatt caataggagg gttcatttat ccaccactag ttataatttt
    41881 ggctaaagaa gaagggttct tagatgagct tggagagtat attattaata aagcatgtat
    41941 tgatttaaag aagagtgaaa agctattgag aaatcctgtt aagttctctg taaatatatc
    42001 tccagaccaa cttgatgacc ctaagttacc agagcaaata aaaaatatca ttagtagtaa
    42061 tgatataaat cctaatatgt taggtataga aataacagag caaattgcat tatcaggttc
    42121 acaaataatt attgaacgta tcaatgcgat acataacttg ggaataaagt taattatgga
    42181 tgattttggg atggggcata gctctcttat atatcttcaa aataataact ttgatatagt
    42241 taaattggat ggttcattag ttaaagagat acttacaaat aaaagaagta gtgaaattat
    42301 aatgactatt gtaaatcttt caaaaaattt agattttgat atagttgttg agtatgttga
    42361 aaatatagac caaagggata agttatatgg tttaggatgt gaaatatatc aaggatatta
    42421 ttatagcaaa gcaatacctt ttgaagagtt tgtagaatat gcaaatcaag aaagagagat
    42481 ataattttat aagagcttgt aaattctagt aatgcataga tttgtaataa aattaagttt
    42541 aggagaaata tatgaatata agtatagtag tagtaggtaa aattaaagaa aaatatttaa
    42601 aattaggtat tgatgagttt aagaaaaggc tttctaagta ttgtaagtta gaaattatag
    42661 agttggatga tgaaaaagct ccagagaatt tgagtgttaa ggaaatggaa attataaaag
    42721 acaaagaagg aaagaaaatt ttagggaaga taaagcataa tagttatgtt atagccttgg
    42781 ctattgatgg taagggttta tcctctgaag agcttgctaa gactatgagt gatttagcag
    42841 ttagaggtaa tagtagtctc tgttttataa taggaggttc tttagggctt tctgatgagg
    42901 ttttaggaag ggctgattat aggttatcct tttctaagat gacttttcct catcagatga
    42961 tgagattgat attgttagaa cagatttata gggcttatag gataaataat ggagaaccgt
    43021 atcataaata aatgatacgg ttttttttag tttgcaactt aataatatta aaataacaaa
    43081 aaagtattga aagtatattg catttaaggt agaattaaat aaaatattat aacattataa
    43141 tgtttatata gttgttaata aaaatttgga ggaaaatata tgataaaaat aatgttggcc
    43201 tgttcagcag ggatgtctac aagtttatta gttactaaaa tggaaagtgc agctaaagaa
    43261 aatggtatag agtcgcaaat ttgggcaata ccagaatcaa ctatacaaaa tgaaatagaa
    43321 aaatgtgatg tattattact aggaccacaa gttagatatg tattacctaa ggctcaagaa
    43381 attgctaagc catataatat accagtagaa gttataaata tgatgcacta tggaacagtt
    43441 aatggagaag ctgttttaaa tagagcgata gaacttaaca acactaagta atataaatta
    43501 taatgtattt ttaatatata aattgggaga atcaaaggat ggatgaaaag attatagaaa
    43561 tatcttttaa tataataggt tatgcaggtg aaggtaaagg attagctttt gaagcaataa
    43621 aagaggctaa gaatggaaat atagaaaaag caaaagaatt attaaaagag tctagggaag
    43681 ttataaataa agcacataga tatcaaacag aattaataca aaacgaagct agtggaaaca
    43741 ggactgaaat aagtgtcata cttatacatg cacaggacca cctaatgaat ggtatgaatt
    43801 ttcaacagtt agctgaagaa atcgttgatt tacatttgaa attacaagat aaataaaaat
    43861 taaagataat aaattaagag agttcttaaa agatacatta tgtgtagcac actaattgtt
    43921 agattattga ttcaatattt agtgagtaat acattaggat tattttgagg actcttttta
    43981 tatttagttt aaagttaata aatatttatt attaataata taagattata tgttataata
    44041 ttataacata taatttaaga ggtgaatcat gttaagaaat caaccattat atatacaagt
    44101 tgtagatata ttaaagcaaa aaatattaaa taacgaatat aaaattgatg aaaatattcc
    44161 aacagaaagg gagttagagc aagagtttgg agtcagtaaa attactatta gaaaagctat
    44221 tgagatatta gaatcagaag ggtatgtaga gaaaaaaagt ggaaaaggga caaaagtaat
    44281 aagtaatagc ctttttaata agctatcaaa agcagagtct ttttcaagta tacttttatc
    44341 taaaggtcat aagttatcta aagagactat ttcaattgaa aaaataataa atgatacaaa
    44401 ttcacaagta tttagtatat ttggagaaaa atgtagcaag attactagaa tgtattattt
    44461 agatagtaag ccatatatat tttttactca ttatattcct tttgatgaaa atctaactgt
    44521 tgaaagtttt aataatgaaa gttctttata tatgtactta tataggagaa atataaatat
    44581 aactagattt gaagatgaat tttttataga agaatcagaa ctagaggtat caaaggcttt
    44641 aaatctagct aaagacatat tattaggaag aaaaagaaag acctatgatg agaatgataa
    44701 tatagttgag ctatcatttg caagatataa tacagaagta caaaactatg taattaaata
    44761 cgaaatataa taagaatcct gagtttattg aaatgataat atgctcagga tttttgttta
    44821 gttgtaaaaa taaaaaatat tataacatta taatgtttac aaccaaacat tataatgtta
    44881 taatattttt aataaacaag tgatattatc aattacataa aaattagttt atgttatgaa
    44941 atcgttattg cattataaat aaaggataat atcaataata cagggggata actatggaaa
    45001 aattcgcaag ttttttagag cgcaagatta tgcctgtagc agcgaagata agtaatcaga
    45061 gacatatgag agctgtaaga gcaggtataa tagcaacatt accactaaca atagtggggt
    45121 catttttcac aatattatta aacataccta ttgatgctta tatggatttt atagctccat
    45181 ttaaagcaat attagatata ccatttagat atactgtagg atttttatct ctatatgcta
    45241 cttttgggat agcatcatca cttgctaaaa gctacgattt agatacaaca ggtgttggta
    45301 tgcttgcagt tatggcattt ttagtttcta caatagttcc aactcaagta gttgaaccag
    45361 taggtggagt tatagaagct ggtagatgga tggatatggc aaaacttaat tcgccatcat
    45421 tatttggagc tatcgtaaca tcgttaatta cagttgaaat atatcgtttt atgaaggaaa
    45481 agaatattac aattaagatg ccagcaggag ttccagtaga ggtttcaaat tcatttactg
    45541 cattattacc gacagcagta attttaatgt tattctgggt aataagacat attttaggat
    45601 ttgatataag tgttgcacta agcgctatgt tatcaccttt aaaaggattc ttatcaggaa
    45661 atagtttagg tggtgggtta ctgacagtat tcctaataac tttattctgg gtacttggaa
    45721 tacatggtcc agctataatg gggccagtaa taagacctat atgggacatg tctatagcag
    45781 aaaatataac tgcattcaca gaaggagtat cagctcatgc aatgccaaac atatttacag
    45841 aacaattttt acagtggtat gtatggatag ggggagcagg ggctactctt gcattagttg
    45901 taatgtttat gttttctaag tcacaatatc taaaaagttt aggtagatta ggatttttac
    45961 cagggttatt caacataaat gaacctataa tatttggagc accaatagtt atgaatccaa
    46021 tactagggat accatttata attggaccta taataatgac aattctatca tatgttctta
    46081 ctattacagg ggttatacct atgatggtag caagattacc atttgcaatg ccagcaccaa
    46141 tagcaacagt aatgagtact aactggagta tactagcagg tatcttagta ttaatcaact
    46201 ttgtgatatc atttgctgtt tattatccat tcttcaaagt ttttgaaaaa caacaattac
    46261 aaagagaaca agaagaaatg agtgaaaatt tataaaaata catagatata gaggttttgt
    46321 actatataaa atatagaaaa agcttataag ttattaaggg tgaaggtagg aggaatgacc
    46381 tttgccttta attttatata ttttaaataa tgtaatttga ggaggagaaa gatggaaaga
    46441 agattaggaa tatcagtata tccagagcat agtaacatgg aagatgataa ggcatatata
    46501 aaaatggctt caaaatacgg atttaaaaga atatttatgt gcatgctttc aattacaaga
    46561 cctaaggaag aagttaaaaa acattttaaa gaaattataa cctatgcaag agaattagga
    46621 tttgaagtaa tccttgatat agctccaaac atatttgata tattacaaat atcttatgat
    46681 gatttaacat tttttagtga gttaaatgct agtggaataa ggcttgacta tggatatgat
    46741 ggaagtaaag aagctatgat ttctttcaat ccatatgatt taaaagtgga actaaatatg
    46801 agtaatgatg tagcatactt ggataatata attacttaca agccaaatgt aaataatata
    46861 tatggttgtc acaattttta tcctcaaagg ggtacaggct tgccatatga tttttttgta
    46921 agatgttcaa atagatttaa aaagtatgga ataagaactg ctgcttttgt taattcaaaa
    46981 tacgcaacaa tagggccttg gaatataaat gatgggctgt gtacgcttga aatgcataga
    47041 aacttagata ttgctacagc aactaagcac ttattcgcaa caggattgat tgatgatgta
    47101 attgtaggga atgcatatgc aagtgaagat gaattaaaaa gaatgtctga agtaaatgaa
    47161 taccaagttg tatttaatgt agtagctgaa gaaaaaactt ctgaagtaga aaagataata
    47221 atggtaaatg agcaacactt tagaagagga gatataacta atcaggtagc aagatctaca
    47281 gaagtaagaa aaaaatatgc taaagaaaat ttccctaaac atgataataa gaacctcttt
    47341 aaaagaggag atgtagtgat tggtaataat gactttggaa agtataaagg ggaattacag
    47401 ttgatacttc aagattgtac agatgaaaga aaaaataagg tgggtagaat aaaggaagac
    47461 gaaataatac ttttagactt tattgaacct tggagtaaat ttaagattat tttagaggga
    47521 gagtaaagtg aagatagaca tattaataaa atgtggaaaa acagtagata aaagtataat
    47581 tgatatagct atattagacg ataaaataat agaagtaaaa aatcagatag atgattctaa
    47641 gtatcaggca aaacaaatta taaacttaga tggagagaaa tttattagtg ctggttggat
    47701 agatatacat acgcattgct acgaaagact agacctttat aaggattatc cagatgaggt
    47761 tggaataaaa agtggagtta ctactgtagt agatgcagga acaacaggag ctttagatat
    47821 aggtaaattt tatgaagata ctaaaaaata taaaacaaat gtatatgctt taataaatat
    47881 tgctaagcag ggaataacat ctcaagatga actttcaagt atgatgaata ttgatgaata
    47941 tgaacttaaa agagcagtta aaaaatacaa agattttgtt gttggaatca aagctagaat
    48001 gagtaaaagt gttgtaattt caaatgatgt tgaaccttta aaagtagcta aaagaataaa
    48061 aaatgaactt aatctaccta tgatggttca ctttgggagt tctcctccaa cgatagagga
    48121 tatatttgac tatatggaaa aaggagatat attaacccat atatataatg gaaagccaaa
    48181 tggaatactt agaggcaatg aggttaaaaa ggaaatcata gaagctagag aaagaggtat
    48241 aattcttgat gtaggtcatg gaaccgaaag ttttagtatg gatattgcaa tgaaatctaa
    48301 ggatgcagga atttttccag acactataag tacagatata tatattaaaa atagaataaa
    48361 tggtccagtg tataatctat ctacaacaat ggaaaagttt atatatatgg gctattcact
    48421 tgaagatata atagataaag ttactaaaaa tgcagcagat gcaataagtt taaaaaataa
    48481 aggtttaata aaagaaggat atgatgctga tttaactata tttgatgtgg ttaatgaaga
    48541 aaaagaactt caagattctt tgaataagtc agtaatcaca agtacgtcta taaagccaag
    48601 agcagtagta gtcaatggag aatatttaaa tataggtaag tctataggag agaatgagta
    48661 gaagggagaa actattgtat gagtagtata tttgaaaaat actctgtaag acgtgttata
    48721 aatgcttcag gcaaaatgac tatactagga gtatcaacag tagataagga agttattgaa
    48781 gcaataggtg aagggtgtaa aaatttcttt ataatagaag aacttataaa taaaacaggt
    48841 gaatatatag caaatttatt agggtgtgaa agtgcactaa tagtttctag tgcatctgct
    48901 gggattgcac agagtgtggg agcaatgata gcaaaagatg atatgagtct agtttacaat
    48961 gtaaataatc ctaagaaaca agttaaaagg gaaataataa ttccaaaggg acataatgtt
    49021 gattatggag ttccagtaga agtaatgata agacttggag gaggagaagt tgttgaggcc
    49081 ggttatgcta atatgtgtaa caaggaacat ataataagag agataaatga aaatacagca
    49141 gcaattatgt atattaaatc tcatcattgt gttcaaaaga gtatgctaag tgtattagat
    49201 gcagtacaag ttgctaaaga atataattta cctttgatag tagatgcagc agcagaagaa
    49261 gatttaaatg tatattatga attgggagca gacctagtga tatacagtgg tgctaaggct
    49321 atagagggtc ctagctcagg gttagtaata ggtaagaaaa aatacataga taatgtaaag
    49381 cttcaatcaa agggtatagg aagagctatg aaaataggaa aagaaaatat tgtaggtcta
    49441 acatgtgcaa ttgagagata tataagtaat gataaagtaa ctttaaaaga aatggaggac
    49501 aagctaaatc catttataga caagataaat actataaagg gagtgagtgc tagtataact
    49561 agggattcag caggtagaga aatacttcgt ggagaaattg actttaatga agatataata
    49621 aataagtcaa cacaacaaat tataaatgaa cttagaagtg gagagatagc tatatacact
    49681 agagactata aggctaatga aggcaagatt gagattgata taagaagtgt aactggaagt
    49741 gatttagata ctatttataa taggataaaa aatatagtag aaagttagag tgtagagtaa
    49801 gtgacattat aatgatttaa taaattaaat tttaccaatg gagaggataa atatgaattg
    49861 tataccacaa tattataaaa atagagtttg tttaaatgtt cttgcaggaa gcataaaaaa
    49921 tgctaaagaa gtatatgatg cttgtgacgg gcatgtattg ataggagtac tatcaaagaa
    49981 ttacagcact gtagaagtag ctattgaaga tatgaaaaaa tactcaaagg aaatagataa
    50041 tgcaatatct gtggggcttg gagctggaga cccaaatcag tcaaatatgg tatctcagat
    50101 ttctaaagaa cttcagccac aacatataaa tcaagtgttt acaggtgtgg ctacgtctag
    50161 agcattgctt ggtcaaaatg atagtataat aaatggatta gtttctccaa ctggtaaacc
    50221 aggatttgta aaaatatcca caggtccatt aagttcaaaa gaaaaagatg gtatagttcc
    50281 tatagaaaca gcaatagcta tgatgaaaga tatgggagga agctcaatta agttttttcc
    50341 aatgggaggg cttaaaacaa aggatgaata tatagaagtt gcaaaagctt gtgcaaagca
    50401 taatttttat ttagaaccaa caggaggtat agacttagat aacttcaaag agatagttca
    50461 aattgcactt gatgctggtg tagaaaaagt tatacctcat gtttatacat caataattga
    50521 taaagaaaca ggtgaaacga aagtggaaga tataggagta ctttataaaa taatgaaaga
    50581 attattggga tagatttatg aatatattag cttttggtga aataatgatg agactttcag
    50641 tacctgacta caagttctta acacaaacca atgaactaaa ttatataatt acaggcacgg
    50701 gattaaatat attatctgga cttaaaaact ttggatacaa tacctatatg ttgacaaagt
    50761 tgcctaataa taatgtaggt aaagcatcaa gtgcaaatat aagaaagctt ggagtaaaag
    50821 atgactttat cacgtatgga gggaatcata taggagtgta ttttcttgaa aatgggtatg
    50881 gagaaagacc ttctgaagtg acttatttga ataggttaaa tagttcattt tgtgagagta
    50941 aattacaaga ttatgatatt gataaatgtt tagaagaaat ggatatagtc catatttgtg
    51001 gtattgctat gcaacttaca gattcagtta aagagattgc tttagaacta gctagaaaat
    51061 cttatgaaaa ggggataaaa gtattctttg attttaattt tagacaatca ttaaatccag
    51121 aaaggactta tgatgattta gtaaatgatt ataagaaaat actaccatat tgtcatgggc
    51181 tatttgctag ttatagagat ataaataaga tacttaatat agatagtcta aatataaaat
    51241 ctagttcaga attgattaaa aaagagtatg atatagatat tgtagctgga actatgagaa
    51301 gatttgatga tatgaataat agatttataa agggatatat atcaaatgat gatggttatt
    51361 ttgaaagtag ggagtataag ttagaaatat atgacagagt tggtgctggg gatggatatg
    51421 tggcaggaat tatatattca tatttggaaa atatgaagag tgaagatata gttgagtttg
    51481 gggttgctag ttcggtgtta gctcatacaa cttatgggga taattcttta gtaagcacag
    51541 gtcatgtact tagactgatg aacaatgaaa aaatagatat attgagataa tacatttgta
    51601 tataataaaa tttttatctt ttttagacac atttttgact tgcttatatt tgttaaacag
    51661 agttattttt aacgattata aatattaaat tagaagttaa ataataaaat agtagaaaat
    51721 agcattaact atagaaaaaa tggttaatgc tatttttatc gttaatattg attaatttta
    51781 tattatagaa atgaactttt ctaatgctgg atttgtattt tttttattcc atacaactac
    51841 aacttcaatt ctattttcta cttcttttat aggtactatt gcaatatcac gttctatcat
    51901 tgtatctgta acacaaaatg aaggtaaaat ggatattccc atgttagaag ataccaatga
    51961 ttcaatagta ttaagattat ttgaacgttg taccacatta ggtacaaacc ctgcttcacg
    52021 atatctattc tgtatcatag atttttgtcc atactcatcc cctgtttcct ttacaaaaat
    52081 aaatttatca tgttgtaatt caattaaatc gatagaatcc tcattgtaaa gtggatgacc
    52141 aggaggtaaa agagctacca aagaatatct ttctacagtt ttatattcaa aatcttttaa
    52201 tggttgattt tctggattaa taacaaaagc gaggtccata gaattatttt ttactaagtt
    52261 taaaagttcg gttatttcat tagtacaaat atccatagat atattagggt atgtattaga
    52321 aaattctttt aaatattgtt gaagtttgtt tttttcatat cctattaagg ttccaatgct
    52381 caaagttcca aaaaaaccat gtgcaaattg ttgtgttttt gcaatagcat cattgatatt
    52441 ttttattata gtacgtgcct cagataaaaa aacttttcca gctggtgtta gttcaacatg
    52501 acgtttactt cttgtaaata gagttacatt tatttgttct tctaaagctt ttatttgctg
    52561 tgtaacagca gtttgagaaa tataaaattt ttctgctgtt ttggtaaaac ttagattttc
    52621 tgctacagat ataaaatatt ctaattgttt agtattcaaa taatcacccc tatttaaact
    52681 tgattgtttg taaaccttat ttatctataa ttaaagctta ttatagtata agaaaattta
    52741 cttttaaaga atattagaaa atatttgtat aaaaatatat gttttaagta tatctttaca
    52801 ttagatttta taaatttatt aataagaata acttatatat tccaatgaaa acgatattgg
    52861 atttaagtaa gtttgtctca taaaatgaaa gtatatcaat tgataatact ttaaaataag
    52921 aagggagaaa aaatggaaga taaattttat gcaaaaggca acggaaataa cggatatatt
    52981 aaaaatcttg aagtttgttc ctttaataac ttagatggaa cttgtggaat gtttcaaatg
    53041 gctctgtaca aaagagatga aaaatactat ttatatggat gctgttttgg aggaaataaa
    53101 aaaaatggag taatgattag cgatattaca gacccttata atccacaatt tataaaacat
    53161 tttcaaatgt tagaccctaa agagtatcct acaacaacaa ctcccaaaat tcaaatagca
    53221 gatgatttaa tgatagtagc aatgagttgt ggaagtggac caggagcact tgttgaccaa
    53281 gctaaattag caaatattaa gtgtgaagca ggaattagaa tatacagttt aaaagaagac
    53341 cctttaaatc ctaagttttt aggatattgg gattgtggct taaagcatgt aatgggtgtt
    53401 catagattca tgtacaatgg tggaagatat gtacatttat caagtgattg tgttggcttt
    53461 gaaggtctga tttatagggt catagatata ataaatccta ctaatccagt ggaaataggt
    53521 aaatggtgga gaccagacca atatgcagat ggatatccaa atagaacttt tgatgcagga
    53581 gcacctcatt gcccagaatt tatggataaa ggatggcttc atggacctcc atttgtaaga
    53641 gacggaaaag catattgtgg ttatggagga gctggtttag ttgtattaga tgttgaagat
    53701 ttaacaagac caagatgctt aggtgaattg ccatttacgc ctgcattttc tagtagactt
    53761 gcaggtgcaa gaactcatac agcattacca ttgccaggaa gagatttagt cgttgttcaa
    53821 aatgagggag aaagattcca gttctttaaa ccagataaca ttacagatgt tcaagctatg
    53881 aataatatac atatggttga tgttagtgac ccaacaaaac caacattaat tgctcaattt
    53941 ccatatcctg aagttccaaa agatttccct tatcctaact ttaatgttgc aggattagga
    54001 aaaccagggc catttggtcc acataatctt catgaaccaa tggataataa gccatggtta
    54061 gagcaaagag gagatagagt atattgctgt tatttccatg cagggctaag ggtttatgat
    54121 gtatcagacc catattatat caaagagcta gcatatttta taccaccaaa tccaaataaa
    54181 acaccagaag aatcttattt cccaggattc ccaggaccac gcttggcagt aacagaagat
    54241 cttatcgttg atgatagagg ctacatcatc atagatgctt tagatgatgg attctatata
    54301 ttaaaaatga aagaagatta aataaatact aaagagggag agttgaaaat gaaaatacta
    54361 ggtatttctt ttggaacaaa aaatggaaat aatgacacaa tgtgtaaagt tgcacttaaa
    54421 ggtgccaaag aagcaggggc tgaagtagaa tttattcaaa tgtcatctat taacattaaa
    54481 cattgtactg gatgttgtgc gtgtgttaaa actttattat ctgggaaagg tagtatgtgt
    54541 gtattaaaag atgattttga atggttgctt gacaaaatga aagatgcaga tggtatagta
    54601 gtttcagacc ctatttttga agaaggggca tcaggattat tccatactat aatggaccgt
    54661 tttggaccca gagcagatag aggaaataac attataggaa caaaagttgc agaagttata
    54721 ggaggaaaaa ttccagattc aagaatgctt aatgataaag taatatcatt tatgggaata
    54781 ggaggttctg actggggtac aagagttcaa tgtgaacatg ctatgctagc tctcatacca
    54841 atgtggaaag ttatagataa tgcttggttc ccatgggcaa aggaattagt aatggatgat
    54901 acaaggcttg ttcaagttca tcaaattggt ttaaatatag ttgaagcagc aaaagatttt
    54961 gaaaaagctt catatcaagg agaagaaggt gtatgtccac attgccacaa tagattattc
    55021 taccttgagc caggtactaa aaaggcaatt tgtgctctat gtggtatagt tggagagtta
    55081 gatactataa gtggaaaaac tatatttagt tttccagaag agcaattagg tcatgctcat
    55141 gatactttac caggtaagtt tatacatggt gatgatatta agaaaatgga aggtcattat
    55201 gcagaagttc gtaaaacaca agaatttaaa gaaagaaaaa catcttataa ttttatagag
    55261 ccattaacta aagaaaagca agctggagtt tcattaggat aataattaaa atactaagac
    55321 tcttttttaa gggatgttag atgttatagg aggcttattg tatgaatgaa aagccaagtt
    55381 ttaaacaaat tgaagttatt ttagcagtta tttttttggt acttacatat tacaaggtta
    55441 gattaactcc agcatttatt gttatattta taccactata tttaagttat tttgcagtat
    55501 ctatgattgt aaatcaaata gcaattaagc tattggagat aaggcaagct agggaagcta
    55561 ctaattacaa tataggaaat ggatagaaag aaaaaagaaa tattaacact gtctatgggg
    55621 attgctttac tacctccttt atgggcagta ttagcacctt atataggaat aaaaactgga
    55681 gctgtagcac taatctgtgc aggattatat gtaactaatg gtaataaaca aaaagatgga
    55741 ttaaaaataa tgtttggttt ttggtgtgga gatttatggg cagtgttagc cattttaatt
    55801 atgggatata tgaatttcaa tcaaaaccta gaattatttt taacacttag catattaggt
    55861 ttttttgcag ttgtaattgc atccttattt gaaaaaataa ttttcctacc atcatggctt
    55921 tgtggttggg ctataggtct aactgttatg acaggtgaaa atattattaa tttacaagat
    55981 atttgtattc aaattgctgt agctatggca gtaggagtat ggtacgtggg tgcaggtgta
    56041 gatttgttta caatatatat tttgagtaaa gataggaaaa agggaaattg aaagcatgtg
    56101 aatatacttt gattatagga gaatggctat gggttttgaa tttaaaataa tgagaagttt
    56161 aatatatgta ggacttgcca aggaagaata tagacctaag ctaatggact ggttatatcg
    56221 tcaccatatt ccagatagta ttagcacttt tggaccatat tgtactaaat atgcctttta
    56281 tcaagcatat cctacaccaa atgaaggtga gcgttttggt gcacgtaaga tgcaactaac
    56341 agaacattat tggcttgtag atgaacatat gcctgagatg gcaaatagaa ttatgacaga
    56401 atatatgcct atggatgttc tacgttggca agggtgtata ccagatgtag aaaataaaag
    56461 ggttcatgaa aatgtagaaa gtggagatgc aggacgtgca gtaggtggag ataatggatg
    56521 tccaccattt atatttgcct ttgttccaat aaactgggaa gaagacttta gaggaaaagg
    56581 acgtactgta caagatggac caaactatcg ttggcaattt atgattaagt atccagatgg
    56641 tatctctaaa gaagaaggag aaaaatggtt ctatgatgag gtagtgccat actttacaaa
    56701 ctgttgctat gttaatcgtt ttgtcagtag taaaataatg attaattatg gagcaactgc
    56761 ttttgaccgt gtatcagaac tatggtttga aggggaagaa gaatggtata aagctgtggt
    56821 tgaagagaca aagtcgttta ttaaaaaacc agaatgggca caagaagagg agttcccata
    56881 tttaaaacca caattcaata tcgcatcagt attcttaggt gatatagcaa ctatggatgc
    56941 atactcacag tatcgtggat atataccaat gagataaaaa ttatagtttg tgataagtag
    57001 aggaggcatt tactcttact ggtagaagaa gacttattgt atccttaaaa gtaggtgaat
    57061 atatagtttt atatttaaat ttatataaaa ctaagactaa taaatatgaa acaatagaat
    57121 tagaataatt tgataatatt tgttaaaaga gcatttagaa tgtgtttaaa gattaagata
    57181 ttccttttat ggtagcagat agttgaaata ataaaactgt tatcatgaaa ggaatatttt
    57241 tatgtatagg aaatttttaa aaataaaagt taaatatgtt taagaacatc ttaaagtgaa
    57301 agaatcattg ttagaaatat gtagacaata tagtctttat aacgtatcat attaacaagt
    57361 ttacacttgg attatgaaat aaaaagagta attccaaagg ttgtaaatac tatgctgact
    57421 ttgatacttc aaagtttagg tgattttgta attgttaata gaaatacgtt taatgagcat
    57481 aaagaattta tctaataata ggagaatata atgtatacaa ttggacaagt atctaaattt
    57541 ttaggaatat ctagagatac actaaaattt tatgaggaca aaggattagt aaatcctaag
    57601 aaaaatgatg aaaatggata tagaatatat aatcaggttg atatctatga tattgcaaca
    57661 ataaactttt atagagaaat tgatatagaa attaaaaaga ttcaagaaat aagaaaaagt
    57721 aagagtataa acaatttaga actcttacta gaagagaagg aacaaatcat actaaaagag
    57781 atagaatata aaaagcttct cttaaaaaag gttcagttag tcaaagaaga ttgtgacaaa
    57841 ataaaaacat attttggaaa atacacaata aaagaaatga agcctttgga agtaaaaggt
    57901 aaaataaaac atgttactaa ctatgaggaa tatgaagccc taaaagagaa tacaaacaac
    57961 ttaaaaaaag cagttacatt aactgattta agaagagtaa taaattttaa tgaagaaggt
    58021 ataatagaag aaaaatttat aattgtaagg gaagtagatg actacaacaa aatcatagaa
    58081 ggtgaagttc tatctcatcc caattgtatt tatactatta ttgaagatgg cagatggtca
    58141 actggaggag aaaatattga taataaagtg gaagacagat taagagaaat agcaatagaa
    58201 aatgaatgtg aattacttgg aatagtctat gttaatatat tgcttactac ttatgaagaa
    58261 gggattgagc gtatattttt agagatttat gcacctataa aataatataa aagcattgac
    58321 ctgggactta ctcataggtt taaactcatg atataagcag ttataactta tatagcaatt
    58381 aaatattaat ttgaaatgag gattacttat gagaacagag aaatcattaa aaaggcctat
    58441 agccagtttt attattctaa caaatataat ttttttacca ctttttttat tggtagtagt
    58501 gactataatg ttaaaacttc caacagtgat atctgacatt gcgttgtgta tatcagcgtg
    58561 gtcatccact tttgcattta tgatattatt taaaaaaatt tatccagata agaaattttt
    58621 agtacacgta aaagatagat ttagaaataa acttaaattt tcaacagtat ctataataat
    58681 tagtatacaa gtcctaatag ctgtagtagt tatatttata ttagcatcta aaaatcatgg
    58741 aataatgcct aactttacag tttcatcatg gggagcattt atatatttat ttttaaaaaa
    58801 tttatttgca ggatcattag gtgaggaatt aggttggaga ggatttgttc aaaatcatct
    58861 tcagaaaaga cattctcctt taaaagcatc cataatagta ggtttttggt gggggatgtg
    58921 gcatttacca atatggttta caacaggatt caaaggactt gatttaataa aatatattat
    58981 attttttatg attgcaatta tatcaatcag tattataatg acaacttttt ataatctaaa
    59041 taaaactttg ataataccta ttgtaataca tcaactattt aattttttaa tagggataat
    59101 taatggagat ttaatagagc ttataaatta ctatgcaatg ttatatttaa tagttgcaat
    59161 tgtattggta gctataaatc caggaaatgc cttatataaa agattttcta aaaacacaac
    59221 acaaatactt taataatata agtgagaatc atatcacaaa tgagagaaat aattctcctt
    59281 attaattaat tttaattaat aaatattaca atagatttat ctggaatgga aggtatgcag
    59341 agtgtatttc attagatgaa acac
//
LOCUS       DBGJFF010000006        56856 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.006, whole genome shotgun sequence.
ACCESSION   DBGJFF010000006 DBGJFF010000000
VERSION     DBGJFF010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 56856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56856
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.006"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            <1..408
                     /locus_tag="ACOIAF_000472"
     CDS             <1..408
                     /locus_tag="ACOIAF_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002578432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HGZ2667503.1"
                     /translation="VNQYLTVPQVSGELAQKIMNEALKYQGWKYVYGGSNPNTSFDCS
                     GLVQWCYGKAGISLPRTAQAQYDATQHIPLSQAKAGDLVFFHSTYNAGSYVTHVGIYV
                     GNNQMYHAGDPIGYADLNSSYWQQHLIGAGRVK"
     gene            428..1330
                     /locus_tag="ACOIAF_000473"
     CDS             428..1330
                     /locus_tag="ACOIAF_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HGZ2667504.1"
                     /translation="MFKKSKKQTENVKEKKVRTVKVGTHKKTVIAFWVVLIVSVSFGV
                     YKNFTAIDMHTVHETETIQLRLTDTNGIENFVKNFAKSYYTWNNSKEAIEARTQAISG
                     YLTKELQDLNVDTIRTYIPTSSTVTDVIVWNIEQSGTDTFSATYEVDQQIKEGEQTTA
                     VKATYTVKVHVDVDGDMVIIQNPTLAPTIEKSDYELKTPEADASVDADTINDATSFLE
                     TFFKLYPTATEKELAYYVSGNVLEPIGKDYLYSELINPVFTKDGDNVKVKVAVKFLDN
                     QTKATQVSQYELVLHKDSNWKIVK"
     gene            1474..2085
                     /locus_tag="ACOIAF_000474"
     CDS             1474..2085
                     /locus_tag="ACOIAF_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HGZ2667505.1"
                     /translation="MPKGIMLTQEQQEERREEIISVALQLIEKNGFQKTSMREIAILA
                     NMGKSSLYDFFKTKDEIFVYAVEKEIEETIKKVHRIIADESSPEQCLRKIMLNHLGVP
                     KQYRTVLMWLNTESDYLEEDYRKRLKTARYAYQDIIKSVIENGMESGVFRKTNANLMT
                     RLLINSIISIIYTSRPSASPEKMLDETMDIFLHGIMNDGGELL"
     gene            2082..4649
                     /locus_tag="ACOIAF_000475"
     CDS             2082..4649
                     /locus_tag="ACOIAF_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HGZ2667506.1"
                     /translation="MIYYTLPLLHKQATLEMVGGKGMSLSKLLTAGIPVPEGFHVTTT
                     SYKIFVEKNHIQPHINKLLDGIDTNNTSQLENVSTQIGMLFHNGEMPQEVSDAIKTAY
                     ARLGNIAVAVRSSATAEDLPDASFAGQQETYLNIQGENEVLDAVKRCWASLWTARAIA
                     YRVKNDIKQEIVALAVVVQKLAFSDASGVMFTLNPINGRRSEMIVNAAWGLGESVVSS
                     LVTPDTIVVDKNAERIVSYEVANKEIMTVRNSDGTEEIPTPEQLRKKHALTRNQVMRL
                     TQLGKKIEKYYEMPMDVEWALEKDKLYIVQARPITVLPPEWTLPEKDVIYTRGSLAEH
                     LPNPVTPLFATLGLEIVNRASALLWIDMFGKSAKKLLPENGAYTIINGYVYLSANSKP
                     LLIAVKSLSPRSLRRALTNSVARWETARKEFEDVIKQWEEKPMHMLNAHQIMEGIQTV
                     FYGACIYFTRIQLTLPAASISETLFTKFFQGAARRAGMTDTSVFLLGFDTIALQAEKN
                     LWNLSEWVKQNNTLNLYLQSNPTAKIAENFMSSVPPDEVSLEVWIEWKNRINQYFKEF
                     GRTAYEFDFAYSTPQETLTPTFESIKTFVEGKGESPFLRQSKFEKHRKQAEEEILQHI
                     GSSRKKLFLKLLHWAQETAPMRENAIYCMGMGHPLIRQMFHEISARLIRGGATSHIDD
                     IYWLTKLELEKLIVQLDKNIPLSDRRNSILERKAELKKYQGYMPPAQLPEKKVKSISH
                     APQKQKDGKTVLRGIGTSSGVVTARACVLNSPADFKNFQPGSVLVAVTTTPAWTPLFA
                     SASGIVTDIGGPLSHSSIVARECGIPAVMATHTATRSIKNGQMITVDGSEGTVTIDE"
     gene            4642..5802
                     /locus_tag="ACOIAF_000476"
     CDS             4642..5802
                     /locus_tag="ACOIAF_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGZ2667507.1"
                     /translation="MNKKPIFALNLATLVIALGYSLILPVMPFYMENLGAGGRELGWL
                     TAVYALAQTVCAPFWGALSDRIGRKPIISIGIIGYSISLFLFGLASSFWTLFIARSLS
                     GILSSATSVASLAYVGDSSSEEERSKNMSQLGASMSVGVMMGPLLGGILAGYSLALPF
                     FTGAGISFIAFLLILTLLPESIERSGQTEKRKPFDFSDLKSIIFGSAGMVLILIFVVH
                     FCQTGLQGITGLYVVDKFGFSTKQVGVIWMVLAGILIVVQGFLTGPLAKKIGEKPLIL
                     IGMFCKALVAFAMVLTTGFVSVLVVFGLFAVSSAITVPTLNATLSKTDNQHKGTLMGF
                     ASTAGNLSKVIAPLLTGYLYESNIELPYITTGGIALIGVVICYIWIKNQKNK"
     gene            complement(5989..6182)
                     /locus_tag="ACOIAF_000477"
                     /pseudo
     CDS             complement(5989..6182)
                     /locus_tag="ACOIAF_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            6284..6790
                     /locus_tag="ACOIAF_000478"
     CDS             6284..6790
                     /locus_tag="ACOIAF_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HGZ2667508.1"
                     /translation="MKNEKKITVALLIVYLLILSWIILFKLQFSFSALDHIRQINLIP
                     FGGSVIANGKIDFDEIINNAIVFIPVGAYFSLLFKNKSVLKAIGSVLGISFVYEIIQF
                     IFAIGASDITDLISNTLGGIIGIALVYVLSIVLKDKTHKILNRIAIVCTVLVVAFLFM
                     LLAVNNIF"
     gene            6795..7043
                     /locus_tag="ACOIAF_000479"
     CDS             6795..7043
                     /locus_tag="ACOIAF_000479"
                     /inference="COORDINATES: protein motif:HMM:NF027483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4865 family protein"
                     /protein_id="HGZ2667509.1"
                     /translation="MLITCCFGKDFNLSKFAFEIEHEITPMEQMKAINFSLEKDDCTS
                     KVLIYNPDKWKYVEYYFYKDRPIKKTIGKIYSILHLSQ"
     gene            7187..7912
                     /locus_tag="ACOIAF_000480"
                     /pseudo
     CDS             7187..7912
                     /locus_tag="ACOIAF_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860758.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            8015..8830
                     /locus_tag="ACOIAF_000481"
     CDS             8015..8830
                     /locus_tag="ACOIAF_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HGZ2667510.1"
                     /translation="MFKIGEFSKLTQVSTRMLRYYDETGLLKPAKIDSLTGYRMYSAE
                     QISTLNRIIYLRDSGFNVAEIALALDCSNDTIIEQLDKKSIEIMDNIQKEQEKLKKIE
                     IAKKELLHGKSELYFNITIKEIPSYCVLSLRKEIPNYYYEGELWKELSAFAKENKINI
                     SDETFSIYHNEEYKEKDVDVELCAIVNKIGKATGDFKFRFTEPVSMMACTMVYGDFSN
                     ISYAYRSFAKWLQENSQYKMKGSSRQIVHRGPWNDKNPENYLTEIQIPLENLL"
     gene            8911..9387
                     /gene="tsaA"
                     /locus_tag="ACOIAF_000482"
     CDS             8911..9387
                     /gene="tsaA"
                     /locus_tag="ACOIAF_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HGZ2667511.1"
                     /translation="MTTYKVNSIGTIYNNEKGAFIQLEAPYILALKELDGFSHINVLW
                     WFSDFDNADFRSVLETDQPYKKAPEKMGIFATRSPVRPNPIALSVVEIINIDYMNGMI
                     QIAYIDANDKTPILDIKPYTPSLDRVENPNVPDWCSHWPHSLEESASFAWENEFNF"
     gene            9401..9952
                     /locus_tag="ACOIAF_000483"
     CDS             9401..9952
                     /locus_tag="ACOIAF_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HGZ2667512.1"
                     /translation="MSLQDYIIEKPVIDTEHLILRTMDKNDIDDLKEWISDISLYEYW
                     GKRPSKSEKNPEVLFSKPEKATKSFHWGIVHKQDSKVIGEIWIYLIENNRMAKVAFRL
                     SKQYQGNGLMSEALKSIIEFCFSKTELQKIWTDVHILNHASYKTLEKVGFQREGHIRG
                     GKMVNTYCDYYLYGILKSDYITS"
     gene            10219..11130
                     /locus_tag="ACOIAF_000484"
     CDS             10219..11130
                     /locus_tag="ACOIAF_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HGZ2667513.1"
                     /translation="MYAYENIEMSLNYIEQHLSEKIETEKLAEIACLSTFYYQRLFKK
                     LVKKSVQEYIKLRRLAMVIAELNNSEERILDIALKYGFSDHANFTRAFKEVYHITPDE
                     YRKNVPMLNTFDKPELSSRYIIIDENVPLIVGNIVLEIQRKTLRTKETYFGFEKTVNI
                     AEQIPAGESTGIDVPGQLWHEFHAKKLQTENAIFNNDIELGVSHLASPDKGTFLYFAG
                     GMVSSTDVCPKNMVQHTLPAGEYIVCKIEAESFEELVTTALNQANKYLFETWLPNHNL
                     VTKPFMAEKYYKGNAEINYMEIWIIPV"
     gene            11208..11642
                     /locus_tag="ACOIAF_000485"
     CDS             11208..11642
                     /locus_tag="ACOIAF_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HGZ2667514.1"
                     /translation="MINIKDINYVPNISEISVYIENSAFDELYQHMNNEYKAVCKIEY
                     SKDVWARGWNVKFKKSGKSLCVVYPKKNYFTVLVVVGNKEKEDVENLLPRLSEYVQEI
                     YRNTREGNGQRWLMIDLKSKNNVYQDVLRLIEIRYAIKCVKP"
     gene            12074..12808
                     /locus_tag="ACOIAF_000486"
                     /pseudo
     CDS             12074..12808
                     /locus_tag="ACOIAF_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
     gene            12801..13411
                     /locus_tag="ACOIAF_000487"
                     /pseudo
     CDS             12801..13411
                     /locus_tag="ACOIAF_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860758.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            13624..14481
                     /locus_tag="ACOIAF_000488"
     CDS             13624..14481
                     /locus_tag="ACOIAF_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGZ2667515.1"
                     /translation="MYKEVGISSNNVPITLSIWYTERSSPTIVFLPGTMSHPLMYENF
                     LCGLSERGFNIIGVHYLSHGKSPKIKKNYIMEDMLHNVYDATTYGIENFGENIAVMGS
                     SQGGILAAMAAGRDTRLKAVFPHNIMLTTLKETMSLTKYPTWSHRFMGLIQWGFRVGG
                     KLLPNYKVPGDAYLDYDRVFYSEQAKQDCLNDPMLLPYYPLRFVASLFNADLSCLENG
                     AIQCPVILITAKGDPLFSFDYTNKVFDLIHVPQKELLALELNRHMIFNEDTDTTINAL
                     EATLHKYLG"
     gene            14559..15140
                     /locus_tag="ACOIAF_000489"
     CDS             14559..15140
                     /locus_tag="ACOIAF_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HGZ2667516.1"
                     /translation="MPRFTEQEKEIINSKLLIEGEKLFALHGLRKVTVDDLVAAVNIS
                     KGSFYAFYPSKEHLYVEINFRLQKELFTSIETTIKKKKYKNHRDLAKDVIMLSLTGVI
                     TSPILSQIDLSLMDYLQRKISSDIFENHMYSDIRILEILEDLGVVFLVPHTVIIKSLY
                     SVLSCLEQFKEDEELNMIQNLLVNGIIQQVVAE"
     gene            15244..16089
                     /locus_tag="ACOIAF_000490"
     CDS             15244..16089
                     /locus_tag="ACOIAF_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2667517.1"
                     /translation="MIDVKKLYFSYTDKPFVENVSFHVGRGEIFGFLGPSGAGKSTIQ
                     KVLTGLNTRYKGSVKVAGTEIREHTNQFYENIGVDFEFSTCYEKFTARQNLAYFASLY
                     EKQPRSIDELLHMVGLENDGDKKVADFSKGMRSRLNFIKALVHDPDILFLDEPTSGLD
                     PTNSRLMKDIILAEKKRGKTIIITTHNMFDATELCDQVAFIVAGKVSALDSPHNLIMS
                     RGAAKIQYTYYDKGEKTGECLLDRTTEDKLLKSLISENRLLSIHSSEPTLNDIFVDIT
                     GRTLQ"
     gene            16086..16769
                     /locus_tag="ACOIAF_000491"
     CDS             16086..16769
                     /locus_tag="ACOIAF_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2667518.1"
                     /translation="MRLGRLICGDIHFQWKYGFYFIYFILTVLYVCGIAALPEHWKTD
                     IASIMIYSDPAAMGLFFMGAIVLLEKSQKVLNAMVVSPVKVSEYILSKTVALIAISTV
                     IAMILGFVSGSNQLLSIAVGTALTSAIFTMLGIIAATKISNLNQFLIVIMPIEIVCFV
                     PPIVGLFVKLPYLFRFFPFTACMNLITGKSVLLSFDMVLVIATLIILYIVARHTVEHM
                     WKSLGGVKL"
     gene            16766..17464
                     /locus_tag="ACOIAF_000492"
     CDS             16766..17464
                     /locus_tag="ACOIAF_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2667519.1"
                     /translation="MKKILSSTIQIFKQIKSDPMMFAACFSPFIMGTLIKFGIPFLER
                     ITKFSLQAYYPIFDLLLSIMAPVLLCFAFAMITLEEIDDKVSRYFSITPLGKSGYLFT
                     RLGVPSIISAVIAFIVLLLFSLEKLSIGMTICLALLGSVQAIIVSLMIITLSSNKLEG
                     MAVTKLAALTLLGIPAPFFIDSYYQFAVGFLPSFWVAKAMQNEAVLYFSIGLVVALVW
                     YYFLTKRLFRKLAG"
     gene            17476..18387
                     /locus_tag="ACOIAF_000493"
     CDS             17476..18387
                     /locus_tag="ACOIAF_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HGZ2667520.1"
                     /translation="MEKGRLLQNKNMNKAIQVSKITLIINLSLSLLKFAAGYIGKSSA
                     MLSDAVHSASDVVSTIVVMVGIKISEKQPDREHPYGHERMECVASIILSVALAITGAG
                     IGYSGIKKIFSGQYNTLSVPSGIALTAAVLSIVIKEWMYWFTRSAAKHTNSDALMADA
                     WHHRSDALSSVGSLIGILGARLGCAILDPIASVVICGCILKAALNIFKESINKMVDHS
                     CDNATETRIREVVLQQQGVDGIDELKTRMFGAKMYVDIEILADGNLALYDAHRIAEGV
                     HQTIENNFPQCKHCMVHVNTNELLYSA"
     gene            18439..18579
                     /locus_tag="ACOIAF_000494"
     CDS             18439..18579
                     /locus_tag="ACOIAF_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667521.1"
                     /translation="MEQYWWIIIVVVLIVVGLLKIVIMKKYNQRRNSNNRSKDSEDDK
                     MY"
     gene            18869..19495
                     /locus_tag="ACOIAF_000495"
     CDS             18869..19495
                     /locus_tag="ACOIAF_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HGZ2667522.1"
                     /translation="MSDTKENILKIALHLFAVNGYEAVSVSMIAEALGMTKGALYKHY
                     KNKQDIFDCIVERMYQIDNKRAKDYQVPESTIEVTPNAYRNTTIGSLITFTLAQYDFW
                     VTDEFASNFRKMLILEQYRNLKMNMLYQKCLVNGPISYIEDIFNEMIEKGVLQECDTK
                     QLAIEYYAPLFLLISVADAVENCWDLKSQLTTHVNQFVKKYITIEKVK"
     gene            19615..20331
                     /locus_tag="ACOIAF_000496"
     CDS             19615..20331
                     /locus_tag="ACOIAF_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HGZ2667523.1"
                     /translation="MEYIRSKKYFTPDLQTKIMGPNPIKLQEELLQNHKIPSGSYVCD
                     LGSGQGLTSVFLAKEYGFVVFAVDLWSEPKENEEFFKKMGLSSAQIKSVKADANELKF
                     EKEFFDAVVSTDSYNYFGRDPIYLDEKLLPFVKKGGFVYITVPGMKKDCHEKLPEELL
                     LSWTPEELDYIHDITYWSNIISHCKKADLISIYEMESNSEVWSDWIKQNNEYAINDRK
                     AIEAGGGDYLNFIAIILQKK"
     gene            complement(21148..22428)
                     /locus_tag="ACOIAF_000497"
     CDS             complement(21148..22428)
                     /locus_tag="ACOIAF_000497"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HGZ2667524.1"
                     /translation="MKEPIDYLSTLFVGIDIAARTHVVSALDFNQEFFIKMKSVPNTQ
                     QGAQLLENMVADVLAENHQFKYVVISMESTGFYGVHLANFLSSSDLLAPFSVRVYCLN
                     PKEVKNYKKSFNDIGKNDGIDSFVIADFARVGRISIKPWRGSQYLALQRLTRHRMHIT
                     ECIAREKTYMLNNIFLKFSEYALLRNGEHPFSNKYGATAEAILTEYTTNDDIVTSSIE
                     ELVAFINSKSRGRIADPQETAKIVQAAARNSYRLDKCLYEPLTISIASSFNCISSFEK
                     ELKAIDKAILQTVQGLNPGEYTVLNSIPGIGKVYSAGILAELGSIKAFQNANALAKYC
                     GIVWNDNDSGDFEAEDKRMSKAGNRYLRYYIIEAAGSVINHCPEYKAFYEKKYAETRT
                     HQHKRALALTSRKFIRLLFGLLDKGQLYSPEKSR"
     gene            22869..23060
                     /locus_tag="ACOIAF_000498"
     CDS             22869..23060
                     /locus_tag="ACOIAF_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003064779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HGZ2667525.1"
                     /translation="MKKYEYVEINYNAKGVVFLCTDKHKEIIDSYAKTGYHYAGFVPT
                     EIDAKGCMRKIDLIFEKED"
     gene            23084..23314
                     /locus_tag="ACOIAF_000499"
     CDS             23084..23314
                     /locus_tag="ACOIAF_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667526.1"
                     /translation="MIGLKRRDMNYIMKVGAKAQGVSVSEFRAMLQDTIDKTMESTDP
                     DVQANFKRYFGNKRPTPEEYIYTITKKTKVKV"
     gene            23581..24000
                     /locus_tag="ACOIAF_000500"
     CDS             23581..24000
                     /locus_tag="ACOIAF_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009261829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HGZ2667527.1"
                     /translation="MKPSDFQKTIQCQFECKLKKVVKGIIWNYRKELKRRKNKEISFS
                     ELPDITVESLATWDDYETNYTIFNVCGIDIKVLDDELAEALKQLPEKKRNNVLMYYFL
                     EMSDTEIADIMNYTRGAVFKHRHRALETMKEILKEKK"
     gene            24003..24257
                     /locus_tag="ACOIAF_000501"
     CDS             24003..24257
                     /locus_tag="ACOIAF_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGZ2667528.1"
                     /translation="MKKKYKHPSYYLISSAMDGDETAIEKLLAFYDPYISKCCLRPLY
                     DEYGNVYIVVDMELKGRIREALIKMILDFEIDIDIETEEE"
     gene            24632..24835
                     /locus_tag="ACOIAF_000502"
     CDS             24632..24835
                     /locus_tag="ACOIAF_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HGZ2667529.1"
                     /translation="MNNTDVPIWEKYTLSIEEASRYFRIGEKKLRKLAEENLDSGWVI
                     VNGNRIQIKRKQFEKIIDTLDEI"
     gene            24913..26103
                     /locus_tag="ACOIAF_000503"
     CDS             24913..26103
                     /locus_tag="ACOIAF_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006354938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGZ2667530.1"
                     /translation="MSKMKRQDNKGRNLRLGESQRKDGRYVYKYTDVFGKPQFVYAWK
                     LVPTDKTPTGKRDDKSLREKERQIKKDLDDGIDTIGGKMTVCQLYAKKNNQKKNIKRN
                     TEIGRQYLMNALENDPLGMRAIDTVKQSDAKEWAIRMNDKGYSYKTIDNYKRSLKASF
                     YMAIQDDCIRKNPFHFKLSDVLEDDTEPKVILTPEQEEKLLSFMETDNVYSKYRDEVI
                     LLLETGLRISELCGLTTHIDMLNRVINIDHQLLRDTEVGYYISTPKTKNGKRELPLTE
                     RAYQALERILKNRGKAQPLVVDGYSNFLFLNREGLPKVAGNYESMVRGLIKKYNKTHE
                     DKLPNITPHSFRHTYCTNMANKGMNPNTLQYIMGHANITMTLGYYAHGTFQSAKAELE
                     RLAS"
     gene            complement(26291..26887)
                     /locus_tag="ACOIAF_000504"
     CDS             complement(26291..26887)
                     /locus_tag="ACOIAF_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheB methylesterase domain-containing protein"
                     /protein_id="HGZ2667531.1"
                     /translation="MERFNNMVIAIGASVGGTEAILEIIKDLPKSTPGIVVVQHMPAI
                     FTYMYAQRLDKQCIMNVREAKNNDRVEQGNVLIAPGGYQMKLCTDKQGYYVTCEKGER
                     ISGHCPSVDVLFDSVAEVAGKNSIGIILTGMGYDGANGLLKMKENGSFTIGQDENSCI
                     VYGMPMVAFDRGSVMLQLPLGEISNCLIRHINILKQKD"
     gene            complement(26927..27730)
                     /locus_tag="ACOIAF_000505"
     CDS             complement(26927..27730)
                     /locus_tag="ACOIAF_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HGZ2667532.1"
                     /translation="MIKLTDEEFIVLVNHVKKEYGIDLSKKRALIEGRLYNTMIEKKL
                     SSFSQYMNLLFKDKTGNEAINLINRLSTNHTFFMREPQHFEFIQNSILPFWEENNKIR
                     NINIWSAGCSSGEEAYSIAMTLDDYFGYNKELWNIKIIATDISTNSLEKAKRGIYIES
                     NVNNVPELWKKKYFIDNKDGTFKICDKIRKSVIFKPFNLMNDFSYQHFDLIFCRNVMI
                     YFDLKTREELINKFYNATKKDSFLFVGHAEVINRSSTKYKYVKPAIYKR"
     gene            complement(27766..28206)
                     /locus_tag="ACOIAF_000506"
     CDS             complement(27766..28206)
                     /locus_tag="ACOIAF_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HGZ2667533.1"
                     /translation="MAKDEIIKQVLTFYVNDVIYGIELENVIETIRFQSITYVPCLPT
                     YISGVINLRGRIIPVINMHIKYNLPKADYNERTCIIITKVDEYQVGIIVDKVVDVIHI
                     ENLNLLETTNSNNTNINKDIKEIAKVEDNSILILDIRKFLINSI"
     gene            complement(28219..30321)
                     /locus_tag="ACOIAF_000507"
     CDS             complement(28219..30321)
                     /locus_tag="ACOIAF_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HGZ2667534.1"
                     /translation="MRQMLDMFIFETSLLLEQLDEIMIQIEKEKVFTHNNINETFRIM
                     HTIKGSASMMGLDNISALAHSVEDLFYAIREGKIEVLDNDLLFDLVFQSLDFIRRDID
                     ELQSRGCISSDYSNFIEGIEEYVSTLELSSSMKSKDNANLESKDEKEVIDSNFEYKED
                     SDVVKIIFEQGCMMENVRAFMLVFELRDYCEFIDFYPKDVETNAESTEYIKSYGFYIQ
                     FVAKEDKAIVYKTIEDNLYVKEYINISKKEIEMKSTSNNDAVENKLKDNNKVSKSYQN
                     NYTEDEIQEDVLNEKTDMEKDSLSEENEHLPISKQSIMSVNLEKLDLLQNIVGEIVIM
                     ESMVVNSVNSTGVDIDNFNKATRQLHKLTDELQDIVMSMRMIQIAGVFQKMHRIVRDM
                     NKKLDKNVELITIGAETEVDKSVIDNLAEPFMHLIRNAMDHAIEPIEERIAKGKEEVG
                     KIVLSAENLGGEAVISISDDGRGIMRDKILAKAKEQGLLKHQSNEYTDDEVNSLIMTP
                     GFSTKESITEFSGRGVGMDIVRKSIEKVGGSIEVKSKQDEGTTFTIKIPLTLSIVEGM
                     KFKVGDSLFTLPISSIRRTFKLTDLNQIITEGNKTELITIYGACIPIIRLHKIYNIQT
                     KVTNLMNGNMIIIENEHKAVCIFVDEIIGNQQVVVKPFPVYFNRFNIKNKGLSGCTIL
                     GDGSISLIIDADSIIEIY"
     gene            complement(30534..32237)
                     /locus_tag="ACOIAF_000508"
     CDS             complement(30534..32237)
                     /locus_tag="ACOIAF_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HGZ2667535.1"
                     /translation="MKREVKLSKLAIVLLIIIVLAVLAGIFSSISSYKAFEKVEKSIN
                     DMNNLRTQSQYIKDTTREISDVLRRYVFTGEKKNKDIYEKELYGKTRESALNKLEKIG
                     LTKSESNKILEAKEISDSLVPTEQSAIKAVESNDKSKAQSIIFGSDYINSQEKMDSLI
                     NEFQEDIVTRSNSKIVEVKKELQSLILRTIFILSLLIILTIIEYTVIKLKIVIPINKV
                     EKHFSRIASGDLRTSIDVEESKSEIGLLVSSVKKMQSMLLNYIDLIDSTLTSIAKGDL
                     RVEIDQEFIGDFKSIKISLESIINSFNGDFSEINLSAEQVASASEQVAAGAQALSQGA
                     TEQASSVEELASTINEISDGIKKCADSASLACEVSDQSASQVGFGSRCMDDMMTSMGE
                     ISEKSSEISKIIKTIDEIAFQTNILALNAAVEAARAGQYGRGFAVVADEVRSLAGKSA
                     KAAQNTAILIEEAIKTVENGVRIAKETANALDLVVNGVEKTTNYINGISSAVHSQEVA
                     ITQILSGIEQISIVVQMNSATAEESAAASEEMSAQAQILKELVEKFHLKESEVRESSW
                     V"
     gene            complement(32263..33222)
                     /locus_tag="ACOIAF_000509"
     CDS             complement(32263..33222)
                     /locus_tag="ACOIAF_000509"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HGZ2667536.1"
                     /translation="MKNKQQGTILVVDDSVLMCDLIDKTLTKDNFKVKKAFNAIEAKE
                     FIEEFIPDIILLDIILPDNSGFEFCKEVKSNARTADIPIIFITSKNTDTDIVKGFGVG
                     AIDYMVKPFSMTELKARVIAHLEVKKSQDKLKKINNELESSLEKLNRLVVRDYLTGLY
                     NRRHIINQLMEQRRINKYSETSISLILGDIDDFKVINDTYGHEAGDFVLTVISSIIKK
                     NCRQIDTVSRWGGEEFLIMLTNTPIEGAKILSERIRKEIKEFDFNYKEHKIKCTITLG
                     VVEVNSNISLEENISLADKAMYEGKNLGKDCSVVSTMKGLKKI"
     gene            complement(33358..33876)
                     /locus_tag="ACOIAF_000510"
     CDS             complement(33358..33876)
                     /locus_tag="ACOIAF_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HGZ2667537.1"
                     /translation="MRFPDDPYLDYGLIDEGIKYLKFKVDEQDFGVELEKIIEITGNQ
                     EIIFIPELPTYSKGIINLRGKIIPIIDMRLRLKKDELMTTNCMYIVVTEIKDLVVGFV
                     VDRVDDIVSLEKSKISPPPRVTIEYSDYFVSGVGELEGKIITLLDLEKLLTDKDEVLI
                     DELINGFDCTIM"
     gene            complement(33919..34404)
                     /locus_tag="ACOIAF_000511"
     CDS             complement(33919..34404)
                     /locus_tag="ACOIAF_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HGZ2667538.1"
                     /translation="MNREIVVNIADMKIAYRPNVLVTYALGSCVGVCLIDKVAGVGGM
                     LHVMLPYSKDAINIENKCKFADTGINELIKSMENIGANRIYIKAKIAGGAQMFLGSSN
                     SVIASIGHRNVEAVKKTLLGLNIPILAEDTGMNYGRTIRFYTETGELLVDSINKGKKK
                     L"
     gene            complement(34401..35048)
                     /locus_tag="ACOIAF_000512"
     CDS             complement(34401..35048)
                     /locus_tag="ACOIAF_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HGZ2667539.1"
                     /translation="MNAIIKGGVYIINYLELDDVHIDALREIGNIGSGNAITALASMI
                     NSNVEVLIPMVKILEYNEATNLLGGPENKVVCILLDMKGDINGMFMFLLDESITQLML
                     SSLFNKEEAFLDEIEAIEISAIKEIGNIMASSYVNAIASMLNMTISVSIPDICIDMVG
                     AVLNVPMIRFSDVGDKVLFIENKFKMSDNYFTSHILMIPEMSSLKEILVRLGLEV"
     gene            complement(35083..35445)
                     /locus_tag="ACOIAF_000513"
     CDS             complement(35083..35445)
                     /locus_tag="ACOIAF_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HGZ2667540.1"
                     /translation="MENKILIVDDAIFMRMIIKEALSKSGYNNLIEANDGEEACNIFK
                     YEKPDLMLLDITMPKKDGLEVLREIKKLDSSAKVVMCSAIGQENMIVEAIKLGALDFI
                     VKPFKTETLVKVVDNALK"
     gene            complement(36189..36686)
                     /locus_tag="ACOIAF_000514"
     CDS             complement(36189..36686)
                     /locus_tag="ACOIAF_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HGZ2667541.1"
                     /translation="MNYEIVEINEKIVIGISKETTNKDGQAVNDIGELWKKFMGKGIY
                     NAIKGKKNDKTIGLYTDYQGDFTSPYSFVACCEVNSNSDKEKNLEELNTNNTVGESII
                     SKVIPAGKYAKFVIVGGQKEVGDFWFEFWQMDFDRTYISDFEEYQCNTFDTKKQEIHI
                     YIGIK"
     gene            complement(36861..37772)
                     /locus_tag="ACOIAF_000515"
     CDS             complement(36861..37772)
                     /locus_tag="ACOIAF_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGZ2667542.1"
                     /translation="MKINRLTEIIVILLNKKLVTAKELADRFEVSTRTIYRDIETLSM
                     SGVPVYMTKGKGGGISLIEEYSIDKAILSKKDKESLIVALKTLQATKYPEINSVVNKI
                     GSIFGEQNFSNWIEIDFTEWGSNFNEDDKFTKIKEAILRRNTINFNYVNSLSSQTNRT
                     VEPLKLMYKSKTWYLYGFCKLKDDFRIFRISRIRNLSIKDEVFSRKIIEEVCLNDSKV
                     IKENTITLKLRFKEKMLFRVFDDFNKDLITKNEDDTYDVITEFPIGEWIYGYILSFGD
                     NVEVLEPKDVRDNVITRLRELSKIYSL"
     gene            38756..39607
                     /locus_tag="ACOIAF_000516"
     CDS             38756..39607
                     /locus_tag="ACOIAF_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HGZ2667543.1"
                     /translation="MEINKKHFIQVFLTCLILVVICEFIGQRTIAIGKASIVLFPMLY
                     AVIIGLIITPDLLGKKIKALKKVVGEKEINIAGEVVGIALVMLGIKYGTTVGPNIDKI
                     IQAGPAFIAQEFGHILAPIVALPLALMFGMKREAVGATASISREPSLGVISEKYGINS
                     PEGSGVLGTYLMGTVLGTIYFSVLGSISIYSGLHPYALGMACGVGSGSMMTAAAGSLS
                     TMVPPEMADTILAYAATSNMMSSITGIIFLVFVSLPFTNFMYKILEPKFSRNKKTKEI
                     KSTEGEC"
     gene            39611..40081
                     /locus_tag="ACOIAF_000517"
     CDS             39611..40081
                     /locus_tag="ACOIAF_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667544.1"
                     /translation="MFANLNLKKSSYQLLVMSVIGVIILIGQRISVGTSIVTALPGMV
                     MLILAAMAAMIIKDLFPKSIFPAFGFATIIGLLLSMPYSPTSEVFLTNTNNINFMAIT
                     TPLLAFAGISVGNKIEALKEMSWKIVIISLIVFTTIFFACASIAHIVLSIQGKI"
     gene            40094..41404
                     /locus_tag="ACOIAF_000518"
     CDS             40094..41404
                     /locus_tag="ACOIAF_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HGZ2667545.1"
                     /translation="MNKEQLKSLVIKTIDENREKILKIGQGIYKNPEYGYKEFKTTEA
                     VSNFFEQELNLSVEKNIAVTGCKADANSSKKGPHISILGELDGISCKEHKDANEIGAS
                     HTCGHNIQIAGMLGAAVGLVKSGVLEHLDGKVSFMATPAEEFIELEYREQLKKEGKIT
                     YFGGKQELIKRGYFDDIDISMMFHASDLGEDKALVGPESNGFVGKKVKFIGKESHAGS
                     APHDGINALNAAMLAINNVNALRETFKESERVRFHPIITKGGDIVNVVPADVHMESYV
                     RARTINGMIDANERINKALMAGGMAVGADVEITEIPGYLPILRYKSLDNLFKNNLEDL
                     GLKGKVIDGGDFTGSFDFGDVSHIMPTLHPMIGGVKGALHTRDFEIVDEDLAYIIPAK
                     AMALTVVDLLFDNAKDAKDILDTFTPVMTKEEYLTFLEKHDKTL"
     gene            41616..42362
                     /locus_tag="ACOIAF_000519"
     CDS             41616..42362
                     /locus_tag="ACOIAF_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HGZ2667546.1"
                     /translation="MFNAGNRIINTWLYPIQDGFVLIDTGYENGFKHFKKRISKFNIK
                     IKDIRYIFLTHAHDDHVGFLNQILDESPNTKIVMHDKALEILYKGQNSFIGGCTSRTA
                     FLFCQIMKLFGKGEHSFSPLKQEFEKRCILVSDKNRKEIEKELNGKIIDTPGHTADSI
                     SLLIDDGVLFCGDSAMNGFPSTHKITIFAESKLEFIQSWKTIINTKPKMIYPGHGKPF
                     EYSKLEQNLIYVNKINLYPLHNSKDIHRFL"
     gene            42724..44265
                     /locus_tag="ACOIAF_000520"
     CDS             42724..44265
                     /locus_tag="ACOIAF_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HGZ2667547.1"
                     /translation="MNDNVVKKKNFFDRTLNRIETVGNKLPDPVTIFLGLCVLLLILS
                     SLVGSMGISVVHPGTGETITAVNLLTVEQLQILLGNIVSNFQGFAPLGLVLVTMIGAG
                     VCDKTGLMTATIKASVSKIPETRVTLVVMTIGMLANIASDAGTILFPPLAALVYLGVG
                     RHPLIGLFSGYAAVCLGFAANIMISVNDILAASFTVPAAQMIDSGFQANATMNLFFMI
                     ASTFVLILLATWVTEKIVAPRFGKYEGNAELDVDGSLSDVEKKGLKKAGISILIYVAI
                     IVALSVIGKKPFLADPETGDLLSNNAPLMKGMVPIIALAFFIPGVVYGKTIGKIKKDK
                     DVVSIMASAMSDMGGYIILAFAASQFLQLFTNSNLGLILAVKGGEFLKSAGINGPLLL
                     IGFIILSCFINLFIGSASAKWAILAPIFVPMFMMVGYNPALTQMAYRIGDCITNPLSP
                     LFPYFPIILAFTRKYDKDAGMGTVIANMIPYSISFLIVWTILLILFMVFNIPLGPGIL
                     PSYSM"
     gene            44300..45406
                     /locus_tag="ACOIAF_000521"
     CDS             44300..45406
                     /locus_tag="ACOIAF_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HGZ2667548.1"
                     /translation="MLDKNRLINNFMDMVRIDSPSNQELEMSKWLVNYLKERNIDAII
                     DDAGKKYGGNTGNVIAYIKGEEGSRPLCLCAHMDQVQPCLGVKPILDGNVVRSDGTTT
                     LGADDKAGIAAILEALEHVITEKIPHRDIYLCFTICEEAGMHGVKNFNPDNLPCKDMV
                     ILDSGGAIGSIAYKAPAQQSIKISFHGKKAHAGIEPEKGLNAILVASHAISNMHIGRI
                     DSLTTSNIGKIEGGGATNIVTDKVTLTAEIRSHIPETLEYELNHMEKCCKDAASKFNT
                     TYTFEHNMSYPSFELSRDSHVFKLSEEAIRQVGVVPNPMVIGGGSDANILANLGYNCA
                     ILSLGMYDVHTVNEYVNIDELYDTAKIVYHMIKL"
     gene            complement(45616..46902)
                     /locus_tag="ACOIAF_000522"
     CDS             complement(45616..46902)
                     /locus_tag="ACOIAF_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGZ2667549.1"
                     /translation="MIVGIIGPSDSGFKIKDDLKQIDSDLKTKIYVREKVVDTIEVIS
                     KCEDECDAIIFTGCAVYEFIKSKYEISKPHSFVPRSGTSIMKAFWAIKSANIKLDKFS
                     IDVVDRYVVEDALKEFDIKATSVFCNPFSLEVGESELVEWHIKLFEENKTDIMLTGFG
                     AVYNELKERGYPVFRLQATIQLIKESYDKVKSEYALSKARFSQIAVEILSLIDYKEKI
                     DNYYSDMIKKSDMDKLVVNYVRSIQGSLFLLGRNDYVVFVHKGVADNEYNYDKLFNLK
                     REIKDMGFSLSIGIGTGVTAYQAENNAHKALRHSIDSKEIGIYLVDEDENIRGPLASE
                     NELNYSLMLSDEKVIEISKKTGMSCESVAKIIAINENRKSKIYDSKELAEYLNVSERS
                     ARRILNKIVNAGLGRICAKETSIGGGRPKNITEILF"
     gene            complement(47025..50123)
                     /locus_tag="ACOIAF_000523"
     CDS             complement(47025..50123)
                     /locus_tag="ACOIAF_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HGZ2667550.1"
                     /translation="MDVINLIDELDEIVYVSSLDTYELLYINEIGKKAMGLNDISHLK
                     CYKALQGKDSPCEFCTNSILKEDEYYIWENTNTRTNRHFILKDKLIKWEGKHARLEIA
                     LDITEKEDISKSIAEKLEIKELLVGCIKHLITESNFSDAVNLVLTNIGKFHKADRAYV
                     FEVSEDRTKVTNTYEWCNEGVDEQKQALTNLDINELKNWFLLFEQKGFVIIDDIENIK
                     EEQPIEYEILKPQKIESLMTSSLKQDDKVVGFIGVDNPHKAVGDTSLLTSLSYFLLTE
                     MKKRKVENKLNYMSYYDFLTGAYNRNKYIKYIDNFQKGKVKSIGVVFVDINGLKDIND
                     NFGHANGDSAIVSACVIIKKYFHSYNVYRIGGDEFVVLCEDISKQLFEELVSWLSEEF
                     KDEMIYSVAVGSIWSDDNIDIHKLIKVADERMYQDKKRYYNKNKLRNNEMKVISDLAD
                     STNNILFKHKELVDKVYFDDLMIKGYSKAYEKLAYNTYDIILSICIDTNLITPIYQSE
                     SNIFSFPIKENLEKTFSYIKKHLIHPDDIILFNDNHLLKSLDSLNNSKQDDLELNFEY
                     RRLGIDKKYYWALIYMLKISSKNKLGEKQTHILVAIKNIDYLMQLHKNQKDTLTGLYN
                     YESFCEQISLMLKRYPDKRYAIIMMDIDKFKVINDIYGVRGGDKALIYVAELIQKNIG
                     SNGICCRMYADVFCIFYESYSDRDIHKVIYKIKNSLNRKKFEYMLVPCFGVYRILDNH
                     IPITNACEMAGYAHKKVKKQSINNYIFYDDTIRNDALEEKQMEREMEYALENGQFHVY
                     LQPKYNLNANKIVGAEALVRWVHPEKGIIPPIKFISLFEKNGFIIRLDMYVWEQVCSL
                     IRKWIDLGEEPVPISVNVSRLHLHNPHFKEIVLSLINKYSIPKSFLELELTESLFIKN
                     IKLFTKIIADLRMDGIVLNMDDFGSAYSSLNMLKNINIDTLKIDCGFFNEEGITDREK
                     IVLKHTIAMAKDLDMDVVAEGVETKEQAEFLVSLDCVVIQGYYYCKPIPSADFEKLLY
                     QK"
     gene            50827..50979
                     /locus_tag="ACOIAF_000524"
     CDS             50827..50979
                     /locus_tag="ACOIAF_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667551.1"
                     /translation="MKIFSFLLFVIGICLCIAGFIGHYPVFVGTTLSFLAIAMIAVRQ
                     VKTKKK"
     gene            complement(51271..51975)
                     /locus_tag="ACOIAF_000525"
     CDS             complement(51271..51975)
                     /locus_tag="ACOIAF_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGZ2667552.1"
                     /translation="MEIKPLVLIVEDDKPICKFIKVSLETQNYRCVETDNGGTAISLI
                     HSLDPDLIILDLGLPDIDGIEVIGRVRACAKTNKIIVVSAREHERDKVEALDGGADDY
                     LTKPFSVTELLARVRVALRNKAQQDNINNDAPKSFEVKNLKIDYENHIVSINGEEIHL
                     TPIEYKIIELMSKYSGRVLTHKFIIDKVWGNYYESENQSLRVFMASIRRKIEKNPAQP
                     EYILTEVGVGYRMADE"
     gene            complement(51966..53573)
                     /locus_tag="ACOIAF_000526"
     CDS             complement(51966..53573)
                     /locus_tag="ACOIAF_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HGZ2667553.1"
                     /translation="MKKERLQHEDLKEYDYDEDGYLSKKIIIKDFIKTILVMTIATGI
                     SVVFEKVGFNESNIILVYILGVLFATSITYGYFFGILSSVIGVLSFNFFFTHPLYTFL
                     AYRKDYPMTFCIMLMAAIITSTLTSRIKREVRLSHKREKMIRLLYENNKTLLKAKNKN
                     QIIEFCGSNLVNILNRTVIVTIADENNNLGKPSIYICNNDGSENIFHSVLEKEAIKEA
                     FRTGNPVGIGTKYCKNSSAYYFPIKGQESNVLGVIGVACFKIDIITENEKILLEAVST
                     QVALAIDRENLFEKNKKANLEAERERLRGNLLRSISHDLRTPLTSILGSSSTILENDD
                     VIDKETRVELLKNIYEDTSWLTHSVENILSMTRIDEGKLDIEKRPEVVDEIIAESILR
                     VKKFANSRDIKTNIPDEIIIVHVDVLLIEQVLVNLIDNAIRHTSKNSKIELTVKKESN
                     QVLFEVADNGKGIPNEDIGNIFNRFYTKNKSRNLERRGIGLGLEICKSIVEAHGGEIV
                     AFNNPSGGATFRFSIPMYDEEGKDEWK"
     gene            complement(53827..55005)
                     /locus_tag="ACOIAF_000527"
     CDS             complement(53827..55005)
                     /locus_tag="ACOIAF_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667554.1"
                     /translation="MEKLSDKRRVKAVSMETFVFIVLLAVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLDTVFLIMAMAVLAGALSALLSEFGVISLVNKIFKGLMRPIWGLPGASIA
                     GVVATYLSDNPAIIPFAKDKTFTQYFKKYQVPALCNLGTAFGMGLIVTTFMIAQGKEY
                     VLPAIIGNVGAIIGSIISVRIMLTFTKKYYNYDPKNDTEKQINDKGAKLEEFREIRDG
                     NVFQRTLDAILEGGKLGVEMGMAIIPGVLVVCTLVMLLTFGPSTDPATGQAVYTGAAY
                     EGIKLLPAIGDKISFIIEPLFGFTSPEAIAFPVTALGAVGAAISLVPEFIKSGAITPN
                     DIAVFTAMGMCWSGYLSTHIGMMDALDARPLAGKAILSHTIGGLCAGICAHFIFMLVG
                     "
     gene            complement(55231..55725)
                     /locus_tag="ACOIAF_000528"
     CDS             complement(55231..55725)
                     /locus_tag="ACOIAF_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HGZ2667555.1"
                     /translation="MHLFIEVIAAFFTALSFGVLFNMKGKNLILAGIGGSIAWFSYKF
                     LLNMGVTENLCFFIATVCFAIYCELCARIYMTPATTLSVCCLIILVPGYGIYNTMYSV
                     LTNNYIKAVEYGVSTLSCASSIALGLVFITTVFRKVNLYGVLTKIKENEKYKKSINKI
                     KQQK"
     gene            complement(55726..56487)
                     /locus_tag="ACOIAF_000529"
     CDS             complement(55726..56487)
                     /locus_tag="ACOIAF_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HGZ2667556.1"
                     /translation="MDIDRILNFSSNAGKAMLQSGGETYRVEETIARICQSFGIDHVD
                     VFATPTAVMASVFVDGKLHSAIKRISSRRVDLNLVHEVNSLSRAISINNLDIELCEKI
                     LDEISEDNYYSDLITIFFAGIAAATFSILFGGNVEEFIAAFTVGILTKFVIMYLSKSS
                     LNDFFVNAIGAVIIASCSILILKLGFIKTLDHLIAGAIMLLVPGLALTNALRDLLDGQ
                     LISGLAKLAEVFFVGVSIAVGMFLVLGLYFKLGGI"
BASE COUNT        18896 a         9548 c         8938 g        19474 t
ORIGIN      
        1 gtcaatcaat atctgactgt accgcaggta tcgggagaac tggcacaaaa gataatgaat
       61 gaagcgttga agtatcaagg ttggaagtat gtctatggtg gcagtaatcc gaacacttcc
      121 tttgactgtt cgggacttgt gcaatggtgt tatggaaaag cgggtatctc tttaccgaga
      181 acggcacaag cccagtatga cgcaacccag catatcccct tgtcgcaggc aaaggctggc
      241 gatttggtgt ttttccattc aacctataac gctggttcgt atgtaaccca tgtcggtatc
      301 tatgtcggga ataatcagat gtaccatgca ggcgacccga taggatatgc agacctcaat
      361 agtagctact ggcaacagca cttaatcggt gcaggacgag taaaataaaa gaaaggactt
      421 agaaaatatg tttaagaaaa gtaagaaaca gacagaaaat gtcaaagaaa aaaaggtgcg
      481 tactgtcaag gtaggcacac ataagaaaac tgtaattgcg ttttgggtgg tgcttatcgt
      541 aagtgtaagt tttggggtat ataagaattt taccgccatt gatatgcaca ccgtacatga
      601 aacagaaacc atacaattaa ggcttaccga tactaacggg attgaaaatt tcgtgaagaa
      661 ctttgcgaag tcctattaca catggaacaa cagcaaagaa gcgattgaag caaggacgca
      721 ggcaatcagc ggttatctga caaaggaatt gcaggacttg aatgttgata ccattagaac
      781 ctatataccg accagttcca cggttacaga tgtgattgtt tggaatatcg aacagtcagg
      841 aacggacact ttttctgcta cctacgaagt agatcagcag ataaaagagg gagaacagac
      901 aacagcagtc aaggcaacct atacggtaaa agtccatgtg gacgttgacg gggatatggt
      961 aatcatacag aaccctacgc ttgcaccaac aatcgaaaaa tcggactatg agctaaagac
     1021 accagaagca gacgcaagtg tggacgctga taccataaat gacgctactt catttttgga
     1081 aacattcttt aagctgtacc cgacagcgac ggaaaaagag cttgcctatt atgtatcggg
     1141 aaatgtgctt gaacctatcg gcaaggacta tctttattct gaattgataa acccagtctt
     1201 tacaaaagac ggggacaatg tgaaagtcaa agttgccgtg aaattccttg ataatcaaac
     1261 aaaggcgacg caggtatcgc agtatgaact tgtgctacat aaggatagta actggaaaat
     1321 tgtgaaataa taatttaaag cgtgcatttc ttataagatt tgtacgcttt acttttatgg
     1381 taaaaattag ccttgacaag cgaccgttgg tcgctatata atgaaagcga aacaagaaac
     1441 gaccaatggt cgctataagg aggtatcata aatatgccaa aaggaattat gcttacacaa
     1501 gaacaacaag aagaacgccg agaagaaata atcagtgttg ctttgcagct catagagaaa
     1561 aatggattcc aaaaaacatc aatgagggaa attgcgattt tagcaaacat gggaaagtcc
     1621 agtctgtatg attttttcaa aacgaaagac gagatttttg tatatgcagt tgagaaagaa
     1681 atagaagaaa caattaaaaa ggttcatagg attattgccg atgaatcctc gccggaacag
     1741 tgtcttagaa aaatcatgct gaatcatctt ggagtaccaa agcagtatcg aacggtgctg
     1801 atgtggttaa atacagaatc tgattattta gaagaagatt atcgaaagcg gctgaaaact
     1861 gcgcgttacg catatcagga tattataaaa tctgtaattg aaaatggaat ggaatcaggc
     1921 gtcttccgta agacgaatgc caatcttatg acgcgtttgt taattaactc cattatatca
     1981 atcatttaca catcccgacc atcagccagt ccggagaaaa tgcttgatga aacaatggat
     2041 atatttctac acggaattat gaatgatgga ggtgaattat tatgatttat tatactctac
     2101 ccttgttgca taaacaggca acccttgaaa tggtcggcgg aaaaggtatg tctttatcaa
     2161 aacttttgac agcgggtatt cctgtgccgg agggctttca tgttacgact acttcataca
     2221 agatttttgt tgaaaagaac catattcagc ctcacattaa taagttgctg gacggtattg
     2281 acactaacaa taccagccag cttgaaaatg tatctacgca gatagggatg cttttccata
     2341 atggagaaat gccgcaggaa gtatcagatg caattaaaac cgcatatgcc agattgggaa
     2401 atatagcggt ggctgtccgt tcctccgcaa ccgccgagga tttgcccgat gcttcttttg
     2461 caggccagca ggaaacctat cttaacatac aaggtgagaa tgaagttctt gacgctgtaa
     2521 agaggtgctg ggcttcccta tggacagctc gcgctattgc ttaccgcgtg aagaatgata
     2581 taaagcagga aatcgtcgca cttgctgttg tagtacaaaa gcttgcgttt tccgatgcgt
     2641 ccggcgttat gtttacgcta aaccctatca atggcagacg tagcgaaatg attgttaacg
     2701 ccgcgtgggg acttggcgaa tcggtggttt ctagcttagt cacccctgat acaatcgttg
     2761 tagataaaaa tgctgaacga attgtatcgt atgaagtagc aaacaaagag attatgacag
     2821 tacgcaactc agatggaaca gaagaaatcc caactcctga acagttgagg aaaaaacatg
     2881 ctcttactcg caatcaagtt atgcgattga cacagcttgg aaaaaaaatt gagaagtatt
     2941 atgaaatgcc tatggatgta gagtgggcac tggaaaaaga taagttgtat attgttcaag
     3001 ctcgccccat tactgtccta ccgccggaat ggacgttacc agaaaaggat gttatatata
     3061 caagaggcag tctagcggaa cacttgccaa atcctgttac gcccttattc gccacacttg
     3121 ggcttgaaat agtcaaccgc gcgtcggcgc ttttatggat agatatgttt ggtaaaagtg
     3181 cgaaaaagct actgccagaa aacggagcat atacgattat taacggatat gtttatcttt
     3241 ccgctaattc aaagcctctt ttgattgctg tcaaatccct ttcaccccga tctttacgcc
     3301 gtgcgctcac gaacagtgtt gcacgctggg aaacagcacg aaaagaattt gaggatgtga
     3361 ttaaacaatg ggaagaaaag cccatgcata tgctgaatgc tcatcaaata atggagggga
     3421 ttcagactgt attttatgga gcgtgtattt atttcacgag gattcagctt acattgccag
     3481 ccgcctctat cagcgaaaca ttatttacaa aattttttca gggggcggct cgtcgcgctg
     3541 gtatgacaga tacttctgtt ttcctgcttg gatttgatac gattgctctg caagccgaaa
     3601 agaacctgtg gaatctctcg gaatgggtaa agcaaaataa tactctcaac ctttatctgc
     3661 aaagtaatcc aacagcaaag atagcggaaa attttatgtc ctcagttccg cctgacgaag
     3721 tttcactgga agtatggata gaatggaaaa atcgaatcaa tcagtatttt aaagagtttg
     3781 gtcgtaccgc ttatgaattt gattttgcat attccacacc acaggaaaca cttacaccaa
     3841 cctttgaatc gataaaaacc tttgtagaag gcaaaggtga aagtcctttt ttacgtcaat
     3901 ccaaatttga aaagcatagg aaacaggcag aagaagaaat tttacaacat ataggtagct
     3961 cacgcaaaaa actgtttttg aagttgctcc attgggcgca ggaaactgct cctatgcgtg
     4021 aaaatgctat ttattgcatg ggaatggggc atcctctgat tcgccagatg tttcatgaaa
     4081 tttcagcacg ccttatacgt ggcggagcta cttcgcatat agatgatatt tattggctca
     4141 caaaattgga attggaaaaa ctcatagtac agttagacaa aaatatacct ttatccgata
     4201 gaagaaattc gatacttgag cgaaaagcgg aactcaaaaa gtatcagggc tatatgccac
     4261 cagctcagtt acctgagaag aaagtaaaaa gcatatcaca tgcaccacag aaacaaaaag
     4321 atggaaaaac cgtgttgagg ggtataggaa ccagttctgg tgtcgtgaca gctcgtgcct
     4381 gcgtactaaa tagtccagca gattttaaaa atttccagcc aggaagtgtt ttggttgccg
     4441 ttactacaac tccggcttgg acacccttat ttgcctctgc aagtggaatt gtaacggata
     4501 ttggaggtcc tctcagtcat tcaagtattg ttgcacggga atgtggtatc cctgccgtta
     4561 tggcgacaca cacagccacg agaagtatta aaaatggaca aatgataacg gtggacggct
     4621 cggaggggac ggtgacgatt gatgaataaa aagccgattt tcgctttaaa tcttgctact
     4681 ttggtgattg cgttaggata tagcttaatt cttccggtca tgccatttta catggaaaat
     4741 ctaggtgctg gcggtcgtga gcttggatgg ctcactgccg tatatgccct ggcacaaaca
     4801 gtatgcgcac ctttttgggg tgcactgtcc gaccgaattg gtagaaagcc gattatcagc
     4861 attggtatta taggatattc catcagtttg ttcctgttcg gtttggcttc atccttttgg
     4921 acactattta tagctcgcag tttatcgggt attttgtcat ctgctacgtc cgttgcatct
     4981 ttggcatatg ttggagattc cagctcggag gaagaaagaa gcaagaatat gagtcaactt
     5041 ggagcttcaa tgagcgttgg cgtaatgatg ggaccattat tgggaggcat tcttgcaggc
     5101 tattcgcttg cgctcccatt ttttacagga gctggcattt catttattgc gtttctgcta
     5161 attctaacac ttttgccaga gtcaattgag cgttcaggac agacggaaaa aagaaaaccc
     5221 tttgattttt cagacctgaa aagtataatt tttggctcag caggaatggt tttgatactt
     5281 attttcgtcg tgcatttctg tcaaacaggc ttgcaaggaa ttacggggct gtatgtcgta
     5341 gataagtttg gattttccac taagcaagtt ggtgttattt ggatggtgct agcaggtatt
     5401 ttgattgtag tacaaggctt tttgactggc cctttagcaa aaaaaattgg tgagaagcca
     5461 ctaatactga taggtatgtt ttgcaaagcc ttagtagcgt ttgccatggt attgacaact
     5521 gggtttgtta gtgtgttggt ggtttttggc ttatttgcgg tatcttctgc tattacagta
     5581 cccacattaa acgcaaccct ttcaaaaact gacaatcagc ataaaggaac actgatggga
     5641 tttgccagta ccgccggaaa tcttagcaaa gttattgctc ctttattaac tgggtatctg
     5701 tatgaaagca atattgaatt accatatata accacgggag ggatagcctt aatcggagtg
     5761 gttatctgct atatctggat aaaaaatcaa aagaacaaat gatggaagag gacgtttata
     5821 tggaggttat acacagtaac taatctggta tagaaattca attgaggctt ttctgcttta
     5881 ttaaagtctt gctactactt aaacctataa tatccttaat tcattatgac aaattccaat
     5941 tcaacagttt tgatacacat aaggaggtaa caatactatg gaaaattatc aatgaagtgc
     6001 taattcttta tcagtgaagt tatccataag tttttctacc tgtaatcgtc tggtgctttt
     6061 tgccagatca ttggtaggta agaaaaattc atcaacagac acatgaagta atgatacaag
     6121 gtcacttgta tgcttggatg ttgtccttta ttttcaatat tagttaagta acgtgggtca
     6181 atatttgtaa ttcgatttta gggaaatatt taaagaaata ttttcgtttt atttctttaa
     6241 atcttcaaga atattgatag ttttttgaaa gtaggtgtgt attatgaaga atgagaaaaa
     6301 aattacagtc gcattgctca ttgtttattt attgatttta tcatggatta ttttatttaa
     6361 gctacaattt tcattttctg cgttagacca cataagacaa ataaacttaa ttccttttgg
     6421 tggttcagtt attgcaaatg gaaaaattga cttcgatgaa attataaaca atgccattgt
     6481 ttttatccct gtaggtgcat atttcagttt gctttttaaa aataaatctg ttctaaaagc
     6541 aataggttct gttttaggaa ttagctttgt atatgagata attcaattta tatttgctat
     6601 cggagcaagt gatattacag acttaattag caacacatta gggggtataa ttggaattgc
     6661 attagtatat gtattatcta ttgttctaaa ggataaaaca cataagatat taaatcgaat
     6721 agcaatagta tgtactgtat tagtagttgc cttcttattt atgcttcttg ctgtaaataa
     6781 tatattttaa tgccatgtta attacatgtt gttttggaaa agactttaat ctgtcaaagt
     6841 ttgcttttga aattgaacat gaaattacac caatggaaca aatgaaagct ataaattttt
     6901 cattggagaa agatgactgt acaagtaaag tattaatata caatccagac aaatggaaat
     6961 atgtagaata ctatttctat aaagatagac ctattaaaaa aactattgga aaaatttatt
     7021 caattttgca tttgtcacaa taaaatatgt tttgaaatat tgaatactta aatatgcaaa
     7081 aaaacaaatg taactggcta tggggctata tctactaaaa agctatgtga taggttggga
     7141 ttaaatttaa acatcacttc tagaaggcat gaatacacaa ggctgacaaa ctatacagtt
     7201 atgattaaac gataccaacg ggattgaaaa tttcgtgaag aactttgaga agtcctatta
     7261 cacatgagca acagcaacga agctattgaa gcaaggacgc aggcaatcta gcggttatct
     7321 gacaaaggag ttgtaggact tgaatgtaga tactatcaga acggatatac cgaccagctc
     7381 tgcggttaca gatgtgattg tgtggaatat cgagcagtcg ggaacggaca ctttatctgc
     7441 tacctacgaa gtagatcagc agataaaaga gagagaacag acaacagcgg tcaaggcaac
     7501 ctataccgta aaagtccatg tggacgctga cggggatatg gtaatcgttc agaaccttac
     7561 tcttgcacca gcaatcgaaa aatcagacta tgagccaaag atatcagaaa cagacgcaag
     7621 cgtggacgct gataccgtta atgacgctac cgcatttctg gaaacattct ttgaggtgta
     7681 cccgacagcc acagaaaaag aacttgccta ttatgtatcg gaaaatgtga ttgaacctat
     7741 cggcaaggac tacctttatt ctgaattggt aaatcctatc tttactaagg acggggacaa
     7801 tgtgaaagtc aaggttgctg tgaagttcct tgataatcag acaaaggcga cgcaggtatc
     7861 gcagtatgag cttgtgttac ataaggatag taactggaaa attgtaggat aacatggaaa
     7921 tgggcttgta tcataattat ggtatgcaag ccctttttat attgaagtat aaattaacaa
     7981 taattataat taggtacatg aatggaggga taaaatgttc aaaattggtg agttttcaaa
     8041 attgacacaa gtttctactc gtatgttgcg ttattatgat gaaacaggtt tattaaaacc
     8101 tgctaaaatt gattcattaa ctggatatcg tatgtattca gcagaacaaa tctcaacttt
     8161 aaatagaatt atatatttac gtgatagtgg tttcaatgtc gctgaaattg cattagccct
     8221 cgattgcagt aatgatacca ttatagaaca gcttgataaa aagtctattg aaattatgga
     8281 taatatacaa aaagaacaag aaaaactaaa gaaaattgag attgctaaaa aagaattgtt
     8341 gcatggcaaa agtgaattgt attttaacat aaccataaaa gagataccga gttattgcgt
     8401 tttatcttta agaaaagaaa taccaaatta ttattatgag ggcgaactct ggaaagaact
     8461 ctctgctttt gctaaagaaa ataaaataaa tatttctgat gaaacattct caatatatca
     8521 caatgaagaa tataaagaaa aagatgttga tgtagaatta tgtgccattg taaataaaat
     8581 aggaaaagct acaggagatt ttaagtttcg ttttaccgaa ccagtttcta tgatggcttg
     8641 tactatggta tatggagatt tttctaatat ttcttatgct tatcgttcat ttgctaaatg
     8701 gcttcaagaa aacagtcaat ataagatgaa aggttcaagc cgtcaaatag ttcatcgtgg
     8761 accgtggaac gataaaaatc cagagaacta tctaacagaa atccaaattc ctttagaaaa
     8821 tctcttgtaa aaaatgataa tctgccttga ctttaacatg gtgttaaggt ttatcgttta
     8881 tttatcaaga agaaaacgga ggaaaatata atgacaactt ataaagtaaa ttcaataggt
     8941 actatctaca acaatgaaaa aggtgctttc attcaactag aagcaccata tattctagca
     9001 ttaaaggaat tagacggatt cagtcatatc aatgttttat ggtggttcag cgattttgac
     9061 aatgcggatt ttagaagcgt attggaaaca gaccaacctt ataaaaaagc tccagagaaa
     9121 atggggattt ttgcaacaag gtcgccagta cgcccaaatc cgattgcttt atcagtagta
     9181 gaaattatca atatcgatta tatgaatgga atgatacaaa ttgcttacat tgatgctaat
     9241 gacaaaacgc ctatcttgga tattaaacca tacacaccga gtttggatag ggtggaaaat
     9301 ccaaatgttc cagattggtg tagtcattgg cctcatagtt tagaagaatc cgcttctttt
     9361 gcatgggaaa acgaatttaa tttttaagga gtgatgaacc atgagtttgc aggattacat
     9421 tattgaaaaa ccagtaattg atacagaaca ccttatttta agaacaatgg ataaaaatga
     9481 catagatgat ttgaaagaat ggattagtga tatttcttta tacgagtatt gggggaaacg
     9541 tccttcaaaa agcgaaaaaa atccagaagt actctttagt aagccagaaa aagctactaa
     9601 aagttttcat tggggaattg tccataaaca agatagcaaa gtgattggtg aaatatggat
     9661 ataccttatt gaaaataata gaatggcaaa agtggctttc agactttcta agcaatatca
     9721 aggtaatgga ctaatgagtg aagcgttgaa aagcataatt gaattttgtt tttcaaaaac
     9781 agaattacaa aaaatatgga cagatgtgca tattttaaat catgcttctt acaagacatt
     9841 ggagaaagtc ggttttcaaa gggaaggaca tattcgaggg ggaaaaatgg taaataccta
     9901 ttgcgactac tacctatatg gaattttaaa atctgattat atcactagct gatgttatgg
     9961 ctaatattga agcgacgatt ggcgaagcaa tgaacaatac agttccaaag gtctatgata
    10021 cgaaaagcat taaaagatat gtgatgaatt tattagatat ggctcattaa aaagtaccaa
    10081 agaaataaaa gttaccatat agacaaaatt ggttttttat atcttttgat ttcttataag
    10141 ataatggcat tgtgcgacaa gctatacaaa aaattttcat gtatactttg attataaatt
    10201 atacaaggag gtactgatat gtacgcatat gaaaatattg aaatgtcatt aaactatatt
    10261 gaacaacatt taagtgagaa aatcgaaaca gagaaactgg cggaaattgc ctgtctttct
    10321 acattttatt accaacgctt gtttaaaaag ttagttaaga aatcagttca agagtatatc
    10381 aaacttcgcc gtttagcaat ggtaatagca gaattgaata attcagagga aagaatatta
    10441 gacatagctt taaaatatgg attttcagac catgctaact tcacaagagc atttaaagaa
    10501 gtttaccata ttactcccga tgaatacaga aagaatgttc ctatgttaaa tacatttgac
    10561 aagccagaac tttcttcacg ttatatcatt attgatgaaa atgtcccatt gattgtagga
    10621 aatattgttt tggaaattca aagaaaaaca ttaagaacaa aggaaacata ttttggtttt
    10681 gaaaaaacag taaatattgc tgaacaaatt cctgctggag aaagtacagg aattgatgtt
    10741 ccaggtcagc tatggcacga attccatgca aagaaactgc aaaccgaaaa tgccattttt
    10801 aacaatgata ttgaacttgg tgtatctcac ttagcaagtc cagataaagg aacattcctt
    10861 tactttgcag gaggtatggt ttcatcaaca gatgtatgtc ctaaaaatat ggtacaacat
    10921 acattaccag caggtgagta tattgtatgc aaaattgagg cagaatcttt tgaagaactt
    10981 gttactactg ctttaaatca agcaaataag tatctgtttg aaacttggct tccaaatcat
    11041 aatttggtta caaagccatt catggcagaa aaatattaca aaggaaatgc agagattaat
    11101 tatatggaaa tatggataat tcctgtgtaa acagtaaatg ctaaatagga attatctgac
    11161 gtaataaaaa ggaaatagaa agcttataaa ggagggcata gtccgttgtg ataaacataa
    11221 aagatataaa ttatgtacct aatattagtg aaataagcgt ttatattgaa aactctgcat
    11281 ttgatgagtt gtatcagcat atgaacaatg aatataaagc agtttgcaaa atcgaataca
    11341 gtaaagatgt ctgggcacga ggttggaacg taaagtttaa aaaatctggg aaatcattgt
    11401 gtgttgtcta tccgaagaag aactacttta ctgtattggt ggttgtaggt aataaagaga
    11461 aagaagatgt tgaaaacttg ttacctaggc tttctgaata tgtacaggaa atttatcgta
    11521 acactagaga gggaaatgga caaaggtggc ttatgataga cttaaaatct aaaaataatg
    11581 tatatcaaga tgtattaagg cttattgaaa ttcgttatgc cataaaatgt gtaaaaccgt
    11641 aataaaaata tttttcacat acgtaatctc aaggtattat cgttgagaca agtaacctat
    11701 ataattatgg gaaatgagat ttttagatac ttaagaaagt actttagggg gcttgcgtta
    11761 tggaaagatt taagtacaat tccacacaat caaatacaat ctaataaagc tttactaaaa
    11821 attgaatgca atacagcttt aaaggctcgt acaaactata tgttttgtgc gggctttttt
    11881 catttaatag gaatttggat tcgtcagaga gtatattata aaaataaaac gataagggta
    11941 ccttaaaaat aaggtacctc aggctgtaga caaagtcccg gactctcgtc tgggcttttc
    12001 ttatatttga gttatccaca attcaacttt ttaagagtta tcttattaag aaaaaagtgt
    12061 atgttttcca cccgatagtt ttacttctgt gatatttgca gatgtcactc aagtagaaag
    12121 tggtggtacg gcagaagatg tcatgcagag ttcagaaagt ctgggactac cgcctaattc
    12181 cttagacacg gaaagctcaa tcaagcaggg gtgtaagtat tttgcgtccc tgctttcttc
    12241 ctgcaaaaat caaggtatcg acgatttgaa tgtagcggta caatcttaca actatggcgg
    12301 gggttatgtg ggctatgtgg caggaaaagg gaaaaagcac acttttaatc ttgcagagag
    12361 cttcgcccgt gagaaatcgg gtggaaagaa agtaacctac gccaacccga tagccgttgc
    12421 aaagaacggg ggctggcggt atggctatgg aaatatgttt tatgtggaat tagtcaatca
    12481 gtatttaact gttccgcagg tgtcggggga actggcacaa aaggtaatga atgaagcgtt
    12541 gaagtatcaa gggtggaaat atgtgtttgg gggaagcaat cccaacactt cctttgattg
    12601 tagcggactt gtgcaatggt gctatggaaa agctggtatc tacttaccga gaacggcaca
    12661 aacacagtat gacgcaaccc agcatatccc tttatcgcag gcaaaagctg gggagttggt
    12721 ctttttccat tcaacctata acgctggttc gtatgtaacg cacgtcggta tctatgtggg
    12781 aaataatcag atgtaccatg caggcaatca gcggttatct aacaaagaaa ttgcaggact
    12841 tgaatgttga tactattaga accgatatac tgactagccc cacagttaca gatgtaattg
    12901 tgtggaatat cgaacagtcg ggaacggaca ctttttctgc tacctacgaa gtagatcagc
    12961 agataaaaga gggagaacag acaacaacag tcaaggcaac ctatacggta aaagtccatg
    13021 tagacgctga cagggatatg gtaattattc agaacctacc cttgcaccag caatcgaaaa
    13081 atcggactat gagccaaaga caccagaagc agacgcaagt gtagacgctg ataccgtgaa
    13141 tgacgctacc gcatttctgg aaacattctt taagctgtac ccgacagcca cagcaaaaga
    13201 gcttgcctat tatgtatttg gaaatgtgct tgaacctatc ggcagggact acctttattc
    13261 agaattggta aacccagtac ttacaaagga cggggataat atgaaagtca aggtagctgt
    13321 aaaattcctt gataatcaga caaaggcgac gcaggtattg cagtatgagc ttattctaca
    13381 taaggatagt aactggaaaa ttattatgta agacattttg ttggcaggat ctattttcgg
    13441 atatagtctg ccttttctgt agcaaatgta ctgcactgac aagctatttc attgtatttt
    13501 aagcaatgaa atctttcttg acaattttac tctaaatcat atatttatag ctattgactt
    13561 cctgattgca ataagatata atatgactat aaaatcaaat cagtcatata ggaggtatga
    13621 tgtatgtata aagaagttgg catttcttca aataatgttc cgataacact ttctatttgg
    13681 tatacagaaa gaagtagtcc taccattgtt tttttgccgg gtactatgtc acaccctctc
    13741 atgtatgaga attttctatg tggattgagc gaaagagggt ttaatataat aggtgttcac
    13801 tatctttcac acggaaaaag ccctaaaatc aaaaaaaatt atataatgga agatatgttg
    13861 cataatgtat atgacgcaac aacttacgga atagaaaatt tcggtgaaaa catagctgtc
    13921 atgggttcca gtcaaggcgg gattttagct gcaatggcgg caggtaggga cacaaggtta
    13981 aaagccgttt ttccacataa tatcatgctg actacattaa aagaaactat gagcttaaca
    14041 aaatatccca catggtcgca ccgttttatg gggctgatac agtggggctt tcgtgtaggt
    14101 ggaaaattgc tgcctaacta caaggttcca ggagacgctt atttagacta tgacagagta
    14161 ttttatagtg aacaggcaaa acaagattgc ttaaatgacc ctatgctttt accctattat
    14221 cctttaagat ttgtcgccag cttatttaat gctgacttat cgtgcctgga gaatggggct
    14281 atacaatgtc ctgttatttt aattactgcc aaaggcgacc cgctttttag ctttgattat
    14341 accaacaaag tatttgattt aattcatgta ccgcaaaagg aattacttgc tttagagctt
    14401 aatcgtcaca tgatttttaa tgaggacacg gacactacaa taaacgcatt ggaagctacc
    14461 ttgcataaat atcttggata gcatattgat tatataatca gtaaatgtta tacttttcat
    14521 gacgatataa atattttagt cataaggagg ttatgagttt gccacgattt acggaacaag
    14581 aaaaagaaat cataaacagt aaacttttga tagagggaga aaagctgttt gcattacacg
    14641 gcttaagaaa agtaacagtt gatgatttag tagctgctgt aaacatatca aaaggttctt
    14701 tctatgcttt ttatcccagt aaagaacatc tatatgttga aatcaatttt cgcttacaaa
    14761 aagagttatt tacaagtata gaaacaacca ttaaaaagaa aaagtataag aaccatagag
    14821 atttagcaaa ggacgttatc atgctaagtt taacgggggt gattacttcg cctattcttt
    14881 cacagattga tttatcttta atggactatc tgcaaagaaa aatatcgtca gatatttttg
    14941 aaaatcatat gtacagtgat attcgtatat tggagatatt ggaagattta ggggttgtat
    15001 ttttggttcc gcatacggtt attataaaat ccctgtattc tgttttatcc tgtttggaac
    15061 aattcaaaga ggacgaagaa ctgaatatga tacaaaatct tttagtgaat ggtattattc
    15121 agcaagtagt agcagagtaa caattttgtt tggttatttg acaggcaggt agccaaacaa
    15181 attttttaca gtttatatga ctatatatct ataaacgtca taaagttaaa ggaggaatga
    15241 ataatgattg atgtaaaaaa actgtatttt agctatactg acaaaccgtt tgtggagaat
    15301 gtaagtttcc atgttgggag aggagaaatt tttggttttt taggaccctc tggtgctgga
    15361 aaatcaacta ttcaaaaagt tttaactggg cttaatacaa gatataaagg cagcgtaaaa
    15421 gttgcaggta cagaaataag ggagcataca aatcagtttt atgaaaatat tggggtagac
    15481 tttgaatttt caacctgcta cgagaaattc acagcacgac aaaatctcgc ctattttgct
    15541 tctctatatg aaaagcaacc tcggtctatt gatgaattat tgcatatggt aggcttggaa
    15601 aatgacgggg acaaaaaagt tgctgacttt tccaaaggta tgcgttctcg cttgaatttc
    15661 attaaagcgt tagtccatga ccctgatata ttattccttg atgaacctac aagcggtctt
    15721 gaccctacaa atagtcgttt aatgaaagat attattcttg cggaaaagaa acgcggaaaa
    15781 acgattatca ttactactca caatatgttt gacgcaacag agctatgtga ccaagtagcc
    15841 tttatcgttg ccggaaaagt cagtgcattg gatagcccgc ataatttgat tatgtcaagg
    15901 ggagctgcca aaatacaata tacctactat gataaaggcg agaaaaccgg agaatgtctg
    15961 cttgatagaa ccacagaaga taagttgtta aaaagtttaa tttccgaaaa ccgtttgctt
    16021 tctattcata gtagtgagcc tacattaaat gatatttttg ttgatattac agggaggaca
    16081 cttcaatgag attgggacgt ttaatttgtg gtgatataca tttccaatgg aaatatggtt
    16141 tctattttat ctattttata ttaacagttt tatatgtgtg cgggattgcc gctttgccag
    16201 aacattggaa aacggatatt gcttctatta tgatctactc tgacccagca gcaatgggat
    16261 tgtttttcat gggagcgatt gttcttttgg aaaaaagtca aaaggtatta aatgctatgg
    16321 tagtatcgcc tgtaaaagta tcggagtaca tactttcaaa aacagttgcg ttaattgcca
    16381 tatctacggt aattgcaatg atattaggat tcgtttccgg tagcaatcag ttacttagta
    16441 tcgcagttgg tacagctttg acctcagcaa tctttactat gcttggaatt attgcagcta
    16501 caaaaatttc aaacttgaac caatttctca ttgtgattat gccgatagaa attgtttgct
    16561 ttgtaccacc tattgttggt ctgtttgtga agttaccata tctgttccgc ttttttccat
    16621 tcacagcttg catgaacctt ataactggaa aaagcgtttt actatcgttt gatatggtac
    16681 ttgtaattgc tacgttgatt atcctctaca tagttgctcg acatacagtt gagcatatgt
    16741 ggaaaagttt gggaggtgta aagctatgaa aaagatatta tccagcacca tacagatatt
    16801 taagcaaatc aaaagtgacc ctatgatgtt tgcggcttgc ttctcccctt ttattatggg
    16861 gactttaatt aagtttggta ttccatttct tgaaagaata acgaagtttt ctttacaagc
    16921 gtattatccg atttttgatt tattactttc tatcatggct cctgtattgc tctgctttgc
    16981 atttgcaatg attacattgg aggaaatcga cgataaagtg tcacggtact tttcgattac
    17041 ccctcttggt aagtcggggt atctctttac aaggttggga gtaccctcaa ttatttcggc
    17101 ggtcatcgct tttattgtac tgttactttt ttcattagaa aagttgtcca ttggaatgac
    17161 aatatgtttg gcgcttctcg gctcagtaca ggcaattatt gtttcactta tgattatcac
    17221 attatcaagc aataaattag agggtatggc agttacaaaa ctagctgcac ttacattgct
    17281 cggaatacca gccccctttt ttattgatag ttactaccag ttcgcggttg gcttccttcc
    17341 atcattttgg gtagcgaaag ccatgcagaa tgaagcggtt ctttatttct ccataggatt
    17401 ggtggtagct ttagtctggt actacttcct tacaaaacgt ctgtttcgga agctggcagg
    17461 ataaggaggc atattatgga aaaaggtaga cttctgcaaa acaaaaatat gaataaggct
    17521 attcaggttt ccaaaattac actaatcatc aatctgagtt tatcgctact taaatttgct
    17581 gccggatata taggaaagtc aagtgccatg ctgtcggacg ctgtacattc ggcttctgat
    17641 gtagtaagca caatcgttgt gatggttgga attaaaatat cagaaaaaca gcctgataga
    17701 gaacatcctt atgggcatga acgtatggaa tgtgtggctt ccattattct ctctgttgca
    17761 ttggctataa cgggtgctgg aataggatat tcaggtatca agaaaatctt ttcaggacag
    17821 tacaatacgc tttctgtccc tagtggaatc gcacttacgg cagcagtctt atctattgtt
    17881 ataaaagagt ggatgtactg gtttacaaga agtgctgcaa agcatacaaa ttctgacgca
    17941 cttatggctg acgcatggca ccaccgctct gatgcactct cgtcggttgg ttccttaatt
    18001 ggcattttag gagcgcgttt aggctgtgct attctcgatc ctattgcaag cgttgtgatt
    18061 tgcggttgta tcttaaaagc tgctttgaac attttcaaag aaagcattaa caaaatggta
    18121 gaccatagtt gtgacaatgc tactgaaaca aggattaggg aagttgtgct tcagcagcaa
    18181 ggggttgatg gtattgatga attaaaaacc cgcatgtttg gagcgaaaat gtatgtagat
    18241 attgaaatat tagctgatgg taatttagct ctttatgacg ctcatagaat tgctgaggga
    18301 gtccatcaaa cgattgaaaa caactttcca caatgtaaac attgtatggt acatgttaat
    18361 acgaatgaac ttctctattc tgcataggac agggaagatg atttaagact attgaaaggg
    18421 aaagggggga agtaggttat ggaacaatac tggtggatta tcattgttgt ggttttgatt
    18481 gtcgttggct tacttaaaat tgtaattatg aagaagtata accaaaggag aaattctaac
    18541 aatcgttcaa aagatagcga ggacgacaag atgtattaag gtttattgaa atctgttatg
    18601 ttgtagaatg atgtttttag atactagaaa gtgctttaag ggggctaacg ttaaggaaag
    18661 ctttgagtac aattctttac aatcaacagt cttttgcata gagaaatgta tgtaggaaat
    18721 atggtgcaag gcataaagca ccagcgatcg agagatcaac ctatgaacca aaggcacact
    18781 acattttata ctacttgaca agtaaacgag cgtttactat aatggtttac agaagtaaac
    18841 gttcgtttac taaagaagga ggttgtaaat gtctgacaca aaagaaaata tattaaaaat
    18901 tgcactacac ttatttgcag tgaatggtta tgaggctgtt tctgttagta tgattgcgga
    18961 agccttggga atgacaaaag gagcattata taaacattat aaaaataagc aagatatttt
    19021 tgactgtatc gtagaacgta tgtatcaaat tgataataag cgagctaagg attatcaagt
    19081 accagaaagc acaatagagg taacccctaa tgcatacaga aatacaacta ttggaagtct
    19141 aataacattt actcttgcac aatacgattt ttgggtgact gatgaatttg cgtctaattt
    19201 tcgcaaaatg ttaatactag agcaatatag aaatttaaaa atgaatatgc tttatcaaaa
    19261 atgtcttgta aatggtccta tcagttatat agaagatata tttaatgaaa tgattgaaaa
    19321 aggtgttctt caagagtgtg atacaaaaca gcttgctatt gaatattatg cgccattatt
    19381 tctattgata agtgtagcag acgcagttga aaattgttgg gatttaaaat ctcaattaac
    19441 cacacatgtt aatcaatttg tcaagaaata tattacaatt gaaaaagtaa aataatcata
    19501 ttctgtcctg aatgcaaaca taacttttta atttcagcag aggaactcaa aatgaatatt
    19561 gttataagtc acaaatatta actattaaaa tattacaata atggagatga gaaaatggaa
    19621 tatattcgca gtaaaaaata ttttacaccc gacttacaga caaaaattat gggaccgaat
    19681 cctataaaat tacaagagga actattacaa aatcataaaa tcccgtctgg cagctatgtc
    19741 tgtgaccttg gaagtggaca aggtttaact tctgtatttc ttgcaaaaga atatggattt
    19801 gttgtttttg cagttgactt atggagtgag ccaaaagaaa atgaagaatt tttcaaaaaa
    19861 atgggattat cttctgcaca aattaaatct gtaaaagctg acgccaatga acttaaattt
    19921 gagaaagagt tttttgatgc tgtagtaagt acagattcat ataattattt tggacgtgac
    19981 cctatatatt tagatgaaaa attgcttcca tttgttaaga aaggtgggtt tgtttatatc
    20041 acagttcctg gcatgaagaa agattgccat gagaaacttc cagaagagtt attgttatca
    20101 tggacaccag aagaacttga ttatatacat gatataacat attggtctaa cataattagt
    20161 cattgtaaaa aggcagattt aatttcaata tatgaaatgg aaagtaattc ggaagtttgg
    20221 tcggattgga taaagcaaaa taatgaatat gcaataaatg accgtaaggc aatagaagct
    20281 ggcggtggag attatctgaa ttttattgct ataattctac aaaaaaaata aattctttag
    20341 aaccaaacac tataatttgc gttctaatat gtttaccgat tgacatcaat aattttatct
    20401 ttgagggtaa tgatttgaaa atagaagaat acaagaagat acgaaattga taaattagaa
    20461 ttttatggag ggaaaaggta atggcaaaag aaaagggtta gttcaaagag tttctatcgg
    20521 cagttgcacc agaatatcaa gcatttgtcg agaaacttaa taacaaactt attgaacaag
    20581 gctgtgacct tgtaatcaag gaagcaaaga gcggttatgc tgcttcatac caactagaga
    20641 agaaaactgt aatgaattgg gtgtttcgga agtcgggggt tttggcacgt atttacggag
    20701 acaatgctgg caagtatgag gatataatcg cttctctgcc tgctgaaatg caaaagaaaa
    20761 tgactacttc tcgtgactgt aagagactta ttgaccctac tgcttgtagc gatacctgtg
    20821 ttaaaggttt tgtttataca ttaaatggcg atacacataa aaaatgccgt aatgacggaa
    20881 tgttcttcct actgacaaac gaaacagcgg agcatatagc taggttggtc tgtgcagagg
    20941 ttactgtgcg taagtcggct tcatagcgac agaatagtta agttcgataa attccagttt
    21001 gtaaaagtca ataaatccaa agataaattt gtatttgaaa gcaatttggt aatatgtcaa
    21061 gaggaaaatg aaaaagattt tctgagaaaa ggataacttt aatagaccta cgattcaaaa
    21121 gaaacgtaag ttcgattcta ttatggatta cctactcttt tccggagagt agagttggcc
    21181 cttgtccagc agaccaaaga gcagacgtat aaatttacgg gaagtcaacg cgagtgctcg
    21241 tttatgctga tgggttcttg tttcagcata tttcttttca tagaaggctt tatactccgg
    21301 gcaatggtta attacactgc cggcagcttc tatgatgtaa tagcgcaggt aacggttgcc
    21361 ggccttgctc atacgtttat cttcggcctc aaaatcgcca gaatcgttat cgttccaaac
    21421 gatgccgcaa tacttggcaa gagcgtttgc attctggaaa gccttgatgg aaccaagttc
    21481 agcaagaata ccagcggaat aaaccttacc aatcccaggt atggaattaa gcactgtata
    21541 ttccccaggg tttaacccct ggacggtttg aaggattgcc ttgtcgattg cttttaattc
    21601 cttttcaaaa gaactgatgc agttgaagga agaagcaatc gagatcgtaa ggggttcata
    21661 aaggcactta tcaagcctgt atgaattacg tgctgcagcc tgtactattt ttgctgtttc
    21721 ttgcgggtca gcaatacgcc cccggctttt ggagttaatg aatgcaacaa gttcttcaat
    21781 ggaagaagtc acaatgtcat cgtttgttgt gtattccgta aggatcgctt cggcagtggc
    21841 gccatatttg tttgaaaacg ggtgctcccc attccgaagc agggcatact cactgaattt
    21901 caggaagatg ttgttcaaca tataagtctt ctcccttgca atacattcag tgatatgcat
    21961 acgatgtctg gtcagccgct gcagggcaag atactgggag ccgcgccagg gcttgatgct
    22021 gatgcggcct acgcgtgcaa aatcagcgat gacaaaggaa tcaataccat cattctttcc
    22081 aatgtcgtta aaggacttct tatagttctt cacttccttt ggattgaggc agtaaacacg
    22141 tacagagaat ggggccaaca ggtcgctgga agataaaaag ttggccagat ggaccccata
    22201 aaaaccggta gattccatgc tgattacaac gtatttgaac tgatggtttt cagccaggac
    22261 atcagcgacc atgttttcaa gaagttgtgc accttgctgt gtgttaggaa cggacttcat
    22321 ttttatgaag aactcctggt tgaaatcaag agcagaaacg acatgggtac gggctgcaat
    22381 gtcaatccca acaaaaagcg ttgagaggta atcaataggt tctttcacgg atcatcacca
    22441 cctttcatag caggaatcaa gaatgtaatc atggcttgtc ccagatcact atgccgccct
    22501 aaacctcgcg ttatcagcat tgtctggcag gaaatccctg ctggtggcta gatgccagaa
    22561 tgcagcattt gtgtaataag tcttggcata gcattagggc tgcaggcttt cttggcaata
    22621 gcataagctt tgctggagtt ttgaagcagt gaccatagct acagttctta tgaaccttat
    22681 tgtaacatct ggggccatga ctataaagta acttattaac caatagacag agaatggatg
    22741 agccagcatt atggaagaag aatcttcgga aaggatgttt agaattatcc ttctgtctaa
    22801 caaaaaaaga ataagtctgt aagctgtttt tcttgcttac aaacttatta tacgaggagt
    22861 tttgaagaat gaaaaaatat gagtatgtag aaattaacta taatgcgaaa ggagttgtat
    22921 tcctttgtac tgataaacac aaagaaatta ttgacagtta cgcaaaaacg gggtatcact
    22981 acgcagggtt tgtacccaca gaaattgacg caaaaggctg tatgcgaaag atagatttaa
    23041 tttttgaaaa ggaagattaa cttattttag ggagttggtt ctaatgatag gacttaaaag
    23101 acgagatatg aattatatta tgaaagttgg tgctaaagct caaggtgttt ctgtatctga
    23161 atttagggct atgctacaag ataccataga caaaactatg gaaagtacag acccagacgt
    23221 gcaggcaaat tttaaacgat actttggaaa taagcgccca acaccagagg aatatattta
    23281 tacgattacc aaaaagacaa aggttaaggt ttaaatgttg taaagggggc ttgccgtatg
    23341 aaacgcttta tgtacaattc agtatattca agcccaattt aacagcatac tatttacaat
    23401 taaatacgct atatcagtag ctcgtacaaa atttatattt tgtgcgggct tttttaatac
    23461 ccgaaaagtt tttttaatta ttttcatttt tttggaagca aatcacacct tgctgtacag
    23521 atatggtgcg gaaagaggga taaacacttt tcaattcata gctggaaagg aggtgagatt
    23581 atgaaaccgt ctgacttcca aaaaacaatt cagtgtcagt ttgaatgtaa gctgaaaaaa
    23641 gttgtcaaag gtattatctg gaactaccgt aaagaattaa aacgaagaaa aaacaaggaa
    23701 atatctttta gcgaattacc agacattacg gtagaaagtc tggcaacatg ggacgactac
    23761 gaaacaaact acacaatttt taatgtgtgt ggcatagata ttaaagtcct tgatgatgaa
    23821 cttgcagaag ctctcaaaca gctacccgaa aaaaaacgta acaatgtatt gatgtattac
    23881 ttcttggaaa tgagtgatac agagattgca gatattatga attataccag aggggctgtg
    23941 ttcaaacaca gacaccgtgc actagaaact atgaaagaga tactaaagga gaagaaatag
    24001 aaatgaagaa aaagtacaag cacccgtctt attatttgat ttcatcagct atggatgggg
    24061 atgaaactgc gattgagaaa ttactggcat tctatgaccc gtacatatca aagtgttgtt
    24121 tacgtccttt atatgatgaa tacggcaatg tttatattgt tgttgatatg gaattaaagg
    24181 gacgtattcg agaagcctta atcaaaatga ttttagactt tgagattgat atagatatag
    24241 aaaccgaaga agaataagca gataccagca gggcgtgatt ggttaaattc ccttctttcc
    24301 tgccctgctc ctgcttttat acatggaagc ctacggcttt cgccgattgt tctttgacaa
    24361 ctgaataaag cagacagata cgtttgtgat acagcgagcc acggggactg tacgccatga
    24421 cctttccgac aagaaagcga gcgacccacg cagttgatcc gagagcgact tgtggaatgg
    24481 tcgttttgcc atgacctgct atcacagaat aatgatacgt ccgcatggtg cggttctgcc
    24541 cacaagaatg ggaatagttg agatactaac ggagctttcc aaagccgtct gtctgcttat
    24601 gataagacac acagaaagga gttgttgcaa catgaacaat actgatgtgc cgatttggga
    24661 aaaatacacc cttagtatag aagaagcgtc ccgatatttt cgtatcgggg aaaagaagtt
    24721 gcgtaaactt gccgaggaaa atctggactc tggttgggtt atcgttaatg gaaaccgtat
    24781 tcagataaag cgaaaacagt ttgaaaaaat tattgataca ctggacgaaa tctgatgtct
    24841 tagagccgta gatatggtat aatattagta gcgtatcacg gctcattcca tgacggaaag
    24901 gagctttaca ctatgtcaaa aatgaaaaga caagacaata aaggacgcaa tttgcgtctt
    24961 ggagagagcc agagaaaaga cggaagatac gtttataaat ataccgatgt attcgggaag
    25021 ccacagtttg tatatgcttg gaaacttgta ccgacagaca aaacacctac tggaaaacgt
    25081 gatgataaat cattgaggga aaaagaaagg cagataaaaa aagaccttga cgacggtatc
    25141 gacactatcg gcggtaaaat gacagtctgc cagctttatg caaagaagaa caaccagaaa
    25201 aagaacatca agagaaacac ggaaatagga cgacagtatc ttatgaacgc actagaaaat
    25261 gacccattag gtatgagggc gattgataca gtcaaacagt ccgatgctaa agaatgggct
    25321 atcagaatga atgacaaagg ttattcctat aagacaatag ataactataa gcgttcctta
    25381 aaggcttcat tctatatggc gatacaagac gactgtatca gaaaaaatcc ttttcatttc
    25441 aagctaagtg atgttctgga agatgataca gagcctaaag ttatcttgac accagagcag
    25501 gaagaaaaac ttctttcctt tatggaaacg gataatgttt acagcaagta tcgtgatgaa
    25561 gttattcttc tgctggaaac gggacttcgt atctctgaat tatgcggact gacaacacac
    25621 attgatatgc ttaatcgtgt gattaacata gaccaccagc tattacgtga tacagaggtg
    25681 ggctactata tttccacacc taagacaaaa aacggcaagc gagagcttcc attgacagaa
    25741 agagcatatc aggcacttga acgaatttta aagaaccgag gaaaagctca accgcttgtt
    25801 gtagacggtt atagcaattt cttgttcttg aaccgtgagg gcttgccaaa ggtagcagga
    25861 aattatgaaa gtatggtgcg tgggctgatt aagaagtaca acaagaccca tgaggataag
    25921 ttgccgaaca tcacgcccca ctcattcaga catacctact gtacgaacat ggcaaataag
    25981 ggaatgaacc ccaacaccct gcaatacatc atgggacacg caaacataac catgacatta
    26041 gggtattatg ctcacggtac tttccagtct gcaaaggcag agttggaaag gctggcttct
    26101 taatatcgaa aatgtatctt actacttatt tactactttt ggttgcattt tcatgctggt
    26161 aaatgccagc ttatgcgagg tatcttccaa aaagaaaatg cccatacagc ccgaaaatac
    26221 gggatatatc ggcatttacc aacttatgaa aagataatca gaaattagta agttttttat
    26281 ttataaaaaa ttaatctttt tgttttaaaa tattaatatg tcttatcaaa cagtttgaaa
    26341 tttcaccaag aggtaattgt aacataacag aacctctatc aaaagctacc attggcattc
    26401 catatactat acaagaattt tcatcctgtc caattgtaaa tgaaccattt tctttcattt
    26461 ttaaaagtcc atttgctcca tcataaccca tacctgtaag tattattcct attgaatttt
    26521 tacctgctac ttctgctact gaatcaaaca aaacgtctac tgatggacag tgaccactta
    26581 ttctttctcc cttttcacaa gttacataat atccttgttt atccgtacac aatttcatct
    26641 ggtaaccccc aggtgcaatt aacacatttc cttgttctac tctatcatta ttcttagctt
    26701 ctcttacatt cataatacac tgcttgtcaa gccgttgtgc atacatataa gtaaatatag
    26761 ctggcatatg ttgaactaca acaatccctg gtgttgattt tggtaggtct tttataattt
    26821 caagtattgc ttctgtacca cctacagatg cgcctatagc aataaccata ttattaaaac
    26881 gttccacatt accgcctcca aaaaatcata tatcaaaaat acctttttat cgtttatata
    26941 tagcaggttt tacatatttg tatttagtac tacttctgtt gattacttct gcatgtccta
    27001 caaacaaaaa actatctttt ttggtagcat tataaaattt atttataagt tcttctcttg
    27061 tttttaaatc aaaataaatc ataacatttc tacaaaaaat caaatcaaaa tgttgatatg
    27121 agaaatcatt cattaaatta aacggcttaa aaattacact ttttctaatc ttatcacaaa
    27181 ttttaaatgt accatccttg ttgtctataa aatacttctt cttccataac tcaggaacat
    27241 tgttaacatt tgattctatg tatatacctc ttttagcttt ttcaagtgag ttagttgaaa
    27301 tatctgtggc tatgatttta atgttccaca actctttatt atatccaaaa taatcatcca
    27361 aagtcatagc tattgaatac gcttcttctc cactagaaca tcctgcactc catatattta
    27421 tgtttcttat tttattattt tcttcccaaa aaggaagtat actattctgt ataaactcaa
    27481 aatgttgagg ttctctcata aaaaatgtgt ggttagtact caatctattt attaggttta
    27541 tagcttcatt tcctgtctta tctttaaata atagattcat atattgacta aaagaactaa
    27601 gttttttctc tatcattgta ttgtataagc gaccttcaat taacgctctt tttttactta
    27661 aatcaatacc atattctttt tttacatggt ttacaagaac tataaattct tcatctgtga
    27721 gttttatcat gcaaaaactt cctcctgcta atttatcaga actaactata tactattaat
    27781 caaaaacttt cgaatatcta aaattaatat agaattatct tcaactttcg caatctcttt
    27841 tatatcttta ttaatattag tgttgttaga attagtcgtc tctagtaaat ttagattttc
    27901 tatatgaata acatcaacaa ctttatctac aataattcca acttgatact catcaacctt
    27961 tgtaattatt atacaagttc tctcattata atcagcttta ggaagattgt actttatatg
    28021 catattaata actggtatta ttcttcctct caaattaata actccactta tatatgttgg
    28081 aagacatggt acatacgtta ttgattgaaa tcttatggtt tcaatcacat tttctaattc
    28141 tattccataa ataacatcat tgacataaaa tgttaacact tgtttaataa tttcatcttt
    28201 agccatactt taaatcccct aatatatttc tataatgcta tctgcatcta taattaaact
    28261 tatacttcca tccccaagta ttgtacatcc cgataaaccc ttatttttta tattaaatcg
    28321 gttaaaataa actggaaatg gtttcacaac aacttgctga ttacctatta tttcatcaac
    28381 aaaaatgcat actgctttat gctcattttc aataattatc atattcccat tcatcagatt
    28441 ggttactttt gtttgaatat tatatatttt atgtaacctt ataatgggaa tacaggctcc
    28501 atatattgta attaactctg tcttatttcc ttctgtaatt atttgattta agtctgtaag
    28561 tttaaatgtt cttcttatag atgatattgg taaagtaaat agtgaatctc caactttaaa
    28621 cttcataccc tctactatag aaagagttaa tggtattttt atagtaaagg ttgtcccttc
    28681 atcctgttta ctttttactt caattgaacc tccaactttt tcaatacttt tacgaacaat
    28741 gtccatgcct actcctcttc cagaaaattc agttatagat tcttttgtag aaaagcctgg
    28801 agtcataatc aaagaattga cctcgtcatc agtatactca ttactttgat gtttaagaag
    28861 accttgttcc ttagcttttg ccaaaatctt atctctcata atgcctctac catcatctga
    28921 aatacttatc acagcttctc cgcctagatt ttcagcagat aaaactatct ttcccacttc
    28981 ttctttacct ttcgctatac gctcctcaat aggctctatt gcatggtcca tagcatttct
    29041 tataaggtgc ataaaaggtt ctgctaaatt atcaattaca ctcttatcta cctctgtctc
    29101 tgctcctata gttattaact ctacattttt atctaatttt ttattcatat ctcgtacaat
    29161 tctatgcatt ttttgaaata caccagcaat ttgaatcata cgcatagaca ttactatatc
    29221 ttgcaattca tctgtaagct tgtgtaattg tcttgttgcc ttattgaaat tatctatatc
    29281 aacacctgta gaatttacgc tatttacaac cattgattcc ataattacaa tttctccaac
    29341 tatattttga agtaagtcca atttttctaa atttacactc ataatactct gcttacttat
    29401 tggtaaatgt tcattttctt cacttagact atctttttcc atatcagttt tttcatttaa
    29461 aacatcttct tgtatctcat cttctgtata gttattttga taacttttac ttactttatt
    29521 attatctttt aatttatttt ctactgcatc attgtttgat gtacttttca tttctatttc
    29581 ttttttacta atatttatgt attcttttac atagagatta tcttctatag ttttataaac
    29641 tattgcctta tcctctttag ctacaaattg tatataaaac ccataacttt taatatactc
    29701 tgtagactct gcatttgttt ctacatcttt tggataaaaa tctataaatt cacagtaatc
    29761 tctaagttca aatacaagca tgaaagctct cacattttcc atcatgcaac cttgttcaaa
    29821 gattattttt actacatcag agtcttcttt gtattcaaaa tttgaatcga ttacttcttt
    29881 ttcgtcttta ctttctaaat ttgcattatc ctttgatttc atagaacttg ataattccaa
    29941 agtactcaca tactcttcaa ttccttcaat aaaattagag taatcagaag atatacaacc
    30001 tcttgattgt aactcatcta tatctcttct tataaaatct aatgattgaa agactaaatc
    30061 aaataatagg tcattgtcta aaacttctat tttaccttca cgtatagcat aaaacaagtc
    30121 ttctacacta tgtgctaaag cagatatatt gtctaacccc atcatagatg ctgaaccttt
    30181 tatcgtatgc attattctaa aagtttcatt aatgttgtta tgagtaaata ctttctcttt
    30241 ttctatttga atcataattt catctaattg ttcgagtaat aaacttgtct caaatataaa
    30301 catatctaac atttgtctca tactcggttc aatataattc acatttatca cctacgacct
    30361 acaaataatt ttctattata tatgcatctc ataatttaaa tccttgatga ttctcttatc
    30421 tcactatatg tacctcataa tttaaataca caattattct cttacttcaa tatatataca
    30481 tctcataatt taaatacacg ataattctct tacttcaata ttcacctcat aatttaaacc
    30541 catgatgatt ctcttacttc actttccttt agatgaaatt tttctactaa ttcttttaaa
    30601 atttgagctt gagcagacat ctcttctgaa gctgctgcac tttcctctgc tgttgctgag
    30661 ttcatttgga ctactatgga aatttgctct attccagata aaatctgtgt aattgctact
    30721 tcttgactat gtacagccga agaaatacca ttaatataat tagtagtttt ttctacacca
    30781 tttactacca agtccaatgc atttgcagtc tctttagcaa ttctgacacc attttctact
    30841 gttttaatag cttcttctat gagaatagct gtattttgag ctgcttttgc acttttacca
    30901 gccaaagacc taacttcatc tgctacaact gcaaatcctc taccatattg acctgctctt
    30961 gctgcttcaa cagctgcatt taatgcaaga atattagttt ggaatgctat ctcatctatt
    31021 gttttgatta tttttgatat ttcactcgat ttttcactta tttcacccat tgatgtcatc
    31081 atatcatcca tacatctact tccgaaacca acttgtgatg cagattggtc agatacctca
    31141 catgcaagac tagcactatc tgcacacttc ttaattccat ctgatatctc attaatcgtg
    31201 gaagctaatt cttctactga actagcttgt tcagtagcac cctgtgaaag agcttgtgca
    31261 cctgctgcaa cttgttctga agcacttgca acttgctctg ctgataaatt tatttcagaa
    31321 aaatcaccat taaaagagtt tattatactt tctaaagata ttttaattga tttaaaatca
    31381 cctataaatt cttggtctat ctctactctt aaatctcctt ttgcaatact agttaatgta
    31441 ctgtctataa ggtctatgta atttaaaagc atactttgca tctttttgac agaactcact
    31501 aatagtccta tttcagattt actttcttca acgtctatac ttgttcttaa gtccccagat
    31561 gcaattcttg aaaagtgttt ttctacttta ttaataggaa tcactatttt taattttatt
    31621 actgtatatt ctattattgt caatattatt aaaagggaaa gtataaatat tgttcttaat
    31681 attagacttt gtaattcttt ttttacttct acaattttgg aatttgaacg ggtaactata
    31741 tcctcttgaa attcatttat taaagaatcc attttctctt gggagtttat atagtcacta
    31801 ccaaaaatta tactttgagc tttactctta tcattacttt cgactgcctt tattgcactt
    31861 tgttctgttg gtactaaaga atctgatatt tcttttgctt ctaagatttt atttgactcg
    31921 ctctttgtaa gaccaatttt ttctaatttg tttaaagctg attctcttgt ttttccgtat
    31981 aattcttttt cataaatatc tttattcttt ttttctcctg taaatacata tcttcttaat
    32041 acatcactta tctctctagt tgtatctttt atatactgac tttgagtcct caaattattc
    32101 atatcattaa tacttttttc tactttttca aatgctttat aagatgaaat agaagaaaaa
    32161 atccctgcta gaacagccaa tactataata atcagcaata caattgctaa tttagaaagc
    32221 tttacttctc ttttcatatc tgtactcctt cataaagtaa gtttatatct tttttaatcc
    32281 tttcattgta ctcactacgc tacaatcttt tcctaagttt ttaccctcat acattgcttt
    32341 atctgctaag gatatgtttt cttctaatga tatatttgag tttacttcta ctactcctaa
    32401 tgtaatagtg cactttattt tatgttcttt atagttgaaa tcaaactcct ttatctcttt
    32461 acggattctt tctgaaagta tcttagcacc ttctattgga gtatttgtga gcattatcaa
    32521 aaactcttct ccaccccatc ttgatacagt atcaatttgt ctacaattct tttttattat
    32581 acttgatatt actgttaaca cgaaatcccc tgcttcatgc ccataagtat cattgataac
    32641 cttaaagtca tcaatatcac caaggataag ggatattgat gtttcactat atttattaat
    32701 tcttctttgc tccataagct gattaattat atgtcgccta ttgtaaagac ctgtaagata
    32761 atctcttaca acgagtctat tcaatttttc aagagaagat tccagttcat tatttatctt
    32821 ttttagttta tcttgagatt tctttacttc aagatgagct ataacacgag cttttaattc
    32881 tgtcatacta aaaggtttca ccatatagtc aattgctcct acaccaaatc cttttactat
    32941 gtctgtatct gtattttttg aagttataaa aattataggt atatctgctg tacgagcatt
    33001 agattttact tccttacaaa actcaaaacc agaattatct ggtaaaatta tatcaagcaa
    33061 aataatgtct ggtataaatt cttcaataaa ttcttttgcc tctattgcat taaatgcttt
    33121 tttaacctta aaattatctt ttgtaagtgt tttatctatt aagtcacaca tcagaacact
    33181 atcatcaacg actaaaattg ttccctgttg cttatttttc atatacaaac ctctcttaaa
    33241 cataatatat tcaaatttta atgatttaaa atttttatac ttaataatta taccactata
    33301 ttcttaaatg ttctcatcac aatcttttta taataacact ccaactacat ccctatatta
    33361 catgatagtg caatcaaacc cattgattaa ttcatctatt aaaacttcat ctttatctgt
    33421 caaaagtttc tctaaatcta gtaaagtaat tattttacct tctaattctc caactccact
    33481 tacaaaataa tccgaatatt ctatagtaac tcttggtgga ggtgatattt tacttttttc
    33541 taagcttact atatcatcaa ctctatcaac cacaaatcca actaccaagt cctttatttc
    33601 agttactact atatacatgc aatttgtagt cattaattca tcttttttaa gtctcaaacg
    33661 catatctatt atcggaatga tttttcctct tagatttatt atgcctttag aatatgtagg
    33721 tagttctgga ataaatataa tttcttgatt tccagtaatt tctattatct tttcaagttc
    33781 aaccccaaaa tcttgctcat ctactttaaa ctttagatac ttaattcctt catcaattag
    33841 tccataatct aaatatgggt catctggaaa tctcatttaa tacctccctc ctaaaataca
    33901 ctttctatga aatatatatt ataatttctt cttaccttta tttattgaat ctacaagtaa
    33961 ttcacccgtc tcagtgtaaa aacgtattgt tctaccataa ttcatacctg tatcctcagc
    34021 taatattgga atatttaatc ctaaaagagt tttttttact gcctcaacat tcctatgtcc
    34081 tatacttgca attactgaat tgctacttcc taaaaacatc tgagcacctc cagcaatttt
    34141 tgcttttata tatattctat ttgcacctat attctccata gatttaatga gctcatttat
    34201 tcctgtgtca gcaaatttac acttattttc aatgttaata gcatctttac tatatggcaa
    34261 cataacgtgt aacattccac caactccagc tactttatct atcaaacata ctcctacaca
    34321 agaaccaagt gcataagtaa ctaaaacatt tggtctatag gctatcttca tatctgcaat
    34381 gttcactact atctctctat tcatacctct aatcccaacc tcacgagtat ctctttaagt
    34441 gagctcattt ctggaatcat caaaatgtga ctagtaaaat aattatcact cattttaaat
    34501 ttattttcta taaataagac tttgtcacca acatctgaaa atcttatcat aggtacattt
    34561 agaacagctc caaccatatc aatacatata tctggaattg aaacacttat agtcatgttt
    34621 aacatagatg caatagcatt tacatatgaa cttgccataa tatttcctat ttccttaatt
    34681 gctgaaattt caattgcctc aatctcatct aaaaaagctt cttctttatt aaaaagagaa
    34741 cttagcataa gttgagttat tgattcatcc aacaaaaaca taaacattcc attgatatca
    34801 cctttcatat cgagtaaaat acatactact ttgttttctg gaccaccaag caaatttgtc
    34861 gcttcattat attcaagtat cttgaccata ggtattaata cttctacatt gctatttatc
    34921 atggatgcaa gtgcagtaat tgcattccca gagcctatat taccaatttc tctaagagca
    34981 tctatatgaa catcatcaag ttctaaataa tttataatat aaacccctcc ctttattatt
    35041 gcatttattt gcttttcatt gtattagtgt atatttacac ttttatttta aagcattatc
    35101 tacaacttta actaatgttt ctgttttgaa gggcttcact ataaaatcta acgctccaag
    35161 ttttatggct tctacaatca tattctcttg tcctattgca gagcacatta caaccttagc
    35221 acttgaatcc aactttttta tttctcttag cacctctaaa ccatcttttt ttggcattgt
    35281 aatatcaagc aacatcaaat caggcttttc atatttaaat atattacacg cttcttctcc
    35341 atcatttgct tctattaaat tattatagcc acttttactc agagcttctt taattatcat
    35401 tctcataaaa atagcatcat ctactatcaa aattttattt tccataaaac taactcctcc
    35461 atatgtacaa caataaacgc ttttatcttc agttattgtc tatactatct gactattttt
    35521 cgctacacaa taaatacatt tcattgataa ataaaagtaa ttatttatgc catattatac
    35581 ttaattctct taacataatt tgtcaacaaa aaaacttata tatctattgt aatatatgtt
    35641 tggcgttttg acgagttatt aaaaatataa ttgtataaaa cataaaaagt tatcaaaagt
    35701 aattaaatta taataaaaca actttatacc ttttttatgt ttgtataaaa aaaattttca
    35761 gattcattaa aatcatttct gtagattcat ataggatttg actttctcaa tatctaatag
    35821 atagttgttt atttacttaa ttatatttct ctatatgtat tatcgattaa attagattta
    35881 gctttataca ttatttttta tatttttcaa attgtatact cataaatcca gaagtattct
    35941 aatgtgttta taatactttt tttcatattt taaattaaat aacaaatcat tatatttaca
    36001 agtatcattt atcaataaac aatattaaat gaagaatatg tctgaagatt caaagtgata
    36061 ctgatactat taattactaa aaaagcagta aatcttttac agatttactg cttatagtta
    36121 taaataataa aactatctat aataaatgta ctatactcaa tatttcattc aatatagtaa
    36181 taacaaatct actttattcc tatgtaaata tgaatttctt gtttctttgt atcaaaagta
    36241 ttacattgat actcttcaaa atcacttata taagtcctat caaaatccat ttgccaaaac
    36301 tcaaaccaaa aatctcctac ttctttttga cctcctacta taacaaactt tgcatactta
    36361 cctgctggta taactttact aataatactt tctccaacag tattattagt gtttaattct
    36421 tctaaattct tctctttatc agaattacta tttacttcac aacatgcaac aaagctatac
    36481 ggacttgtaa aatcaccctg gtaatctgta tatagaccta ttgtcttgtc attcttttta
    36541 ccctttatag cattatatat ccctttgccc ataaattttt tccacaattc acctatatca
    36601 tttaccgctt gtccatcttt atttgtagtc tccttcgata ttcctataac tattttctca
    36661 tttatctcta ctatttcata attcatactt tatacctcac tttgttttta ttaatctatt
    36721 ttattttgaa ctaattcaat tataatttat aatatacgac acctttatgt catattttta
    36781 atataattaa actcagtgag atattatata ctaaatgcaa gatattatat atttaataaa
    36841 atctttaatc tagcattata ttataatgaa taaattttag acaactccct tagtctagtt
    36901 attacattat ccctaacatc tttaggttca agtacttcaa cattgtcacc aaatgataaa
    36961 atatatccat aaatccattc accaatagga aattctgtaa ttacatcata tgtatcatct
    37021 tcatttttag ttattaaatc cttattaaaa tcatcaaata ctctaaaaag catcttttct
    37081 ttaaacctca atttcaacgt tatagtattt tccttaatta ctttagaatc attcaaacat
    37141 acttcttcta ttatctttct tgaaaatact tcatccttta tagataaatt ccttatccta
    37201 ctaattctaa atatcctaaa atcatctttt agtttacaaa atccatacaa gtaccaagtc
    37261 ttactcttat acataagctt cagtggttcg acagttctgt ttgtctggct tgacaaacta
    37321 tttacataat taaaatttat agtattacgc cttaaaatag cttcttttat ttttgtaaat
    37381 ttatcatctt cattaaaatt acttccccat tcagtaaagt ctatttcaat ccagtttgaa
    37441 aaattctgct ctccaaatat tgagccaatt ttatttacta ctgaatttat ttctggatac
    37501 ttagtcgcct gtaatgtttt cagtgcaact attaaactct ctttatcttt ttttgacaat
    37561 atagccttgt ctatagaata ttcttctatt aaagatatcc ctcctccttt gcctttcgtc
    37621 atatatactg gtactcccga catagacaga gtttctatat ctctatatat ggttcttgta
    37681 gaaacttcaa atctatctgc caactcttta gctgttacta attttttatt taatagaatt
    37741 actattattt ctgttaatct atttattttc attcattacc acctactaaa attattaaat
    37801 ttatttaatc taaattaatc atactaattt ttaaataaat aaaatcttag aatcttatct
    37861 aaattatttt tacatgattt tatttacttt ctattatctt tctcactttg gatattatca
    37921 tcttaattta tttattacta tatcaattta cttattatca tctcatttgt tatcgtctta
    37981 cttaatttac ttatttctat atcaattcac ttattattac ctcacttgtt atcatcttac
    38041 ttaatttact tatttctata tcaatttact tattatcatc tcatttgtta tcgtcttact
    38101 taatttactt attgctatgt caattcattt attattatct cacttgttat cgtcttactt
    38161 aatttactta ttgctatgtc aattcattta ttattatctc acttgttatc gtcttactta
    38221 atttacttat ttctatatca attcacttat tatcatctta attcatttga ttattatcat
    38281 ttactttatt caatttctat atcaaaaatt agtatttgta cttaatactt aaaattaatt
    38341 taaagtcttt acatattatt tattttttat tgtaatattc atttccatac tttactaagc
    38401 tacttccact catttatttt aaaatttcaa tttactattt ctcaattcta tattattatt
    38461 tttcaatttt atattactaa ttttaaagct ttttagctat tattcctaca atatttaaac
    38521 aattttttca taatgaagct atattacagt atattcaaaa tatgtaatat tttagatatt
    38581 tttcgttgac aatgttaaat ttttgtttta tccttaatta aggacatgtt attgacattt
    38641 ctgaaactta tttgaaatag ttttttcatt ttatttagga aaacgtatta tacttaagaa
    38701 aacgtatttt ctcatttcag ataagtaaaa tatttattct tttgaggagg aaatcatgga
    38761 aattaataaa aaacatttta ttcaagtatt tttaacttgc ttaatcttgg tagtaatatg
    38821 tgaatttata gggcaaagaa ctatagctat tggaaaagcc tctatagtac tgttccctat
    38881 gctttatgca gttataattg gacttataat aacaccagat ttattaggta aaaaaattaa
    38941 agcgcttaaa aaagtggttg gagaaaaaga aataaatatt gctggtgaag ttgttggaat
    39001 tgcactagta atgcttggta taaaatatgg tactacagtt ggaccaaaca ttgacaaaat
    39061 aattcaagct ggaccagcgt ttatcgctca agaatttggt catatacttg caccaatcgt
    39121 tgcattacct cttgcattaa tgtttggaat gaaaagagaa gctgttggag ctactgcaag
    39181 tataagtcgt gaaccttctt taggagttat atcagaaaaa tatgggataa actcaccaga
    39241 aggtagtggt gtacttggta catatttaat gggaacagtt cttggtacta tatactttag
    39301 tgtactaggt tcaatatcta tttatagtgg tcttcaccca tatgctttgg gaatggcctg
    39361 tggtgttggt agtggaagta tgatgacagc agctgctggt tcattatcta ccatggtacc
    39421 acctgaaatg gcagatacaa tacttgccta tgctgcaaca agcaatatga tgtctagtat
    39481 aacaggaatt atattcttag tttttgtgtc cttaccattt actaatttta tgtataagat
    39541 tttagaacca aaattttcga gaaataaaaa aactaaagaa attaaatcta cagaaggaga
    39601 gtgctaataa atgtttgcta atttaaattt aaagaaaagt tcatatcaat tattggttat
    39661 gtctgttatt ggagttataa ttcttatagg acaaagaatt agcgttggta catctatagt
    39721 tacagctctt cctggtatgg tgatgctaat attagctgct atggcagcta tgattataaa
    39781 agatttattt cctaaatcaa tctttccagc ttttggattt gcaactataa tcggcctttt
    39841 acttagtatg ccatatagtc caacatctga agtattctta actaacacta ataatattaa
    39901 ctttatggct ataactactc cactactagc ttttgcagga atttctgttg gaaacaaaat
    39961 agaagcttta aaagaaatgt catggaaaat agttataatt tcacttattg tatttacaac
    40021 tatattcttt gcatgtgcga gtatagctca tatagtttta agtattcaag gtaaaatata
    40081 ggaggaattt attatgaata aagaacaatt aaaatctcta gtaataaaaa ctatagatga
    40141 aaatagagaa aaaatattaa aaataggtca aggaatatac aaaaatccag aatatggata
    40201 taaagaattt aaaactacag aagctgtttc aaactttttt gagcaagaat taaatttatc
    40261 tgtagaaaaa aatatagcag ttacaggatg caaagcagat gcaaattcta gcaagaaagg
    40321 tcctcatata tctatacttg gagaattgga tggtatctca tgtaaagaac ataaagatgc
    40381 taatgaaata ggtgcttctc atacttgtgg acataatatt cagatagctg gaatgttagg
    40441 tgctgctgtt ggtttagtaa aaagtggcgt tttagaacat ttagatggta aagtatcttt
    40501 tatggctact cctgctgaag agtttattga gttggaatac agagagcaat taaaaaaaga
    40561 aggaaaaata acttattttg gtggaaaaca agaattaata aaaagaggtt attttgatga
    40621 tatagacata tctatgatgt ttcatgcttc tgatttagga gaagataagg ctctagttgg
    40681 tcctgagagt aatggatttg ttggaaaaaa ggttaaattt ataggtaaag aatctcatgc
    40741 tggttctgct ccacatgatg gaataaatgc tttaaatgca gcaatgcttg ctataaacaa
    40801 cgtgaatgca cttagagaaa cttttaaaga gtctgaaaga gttagattcc accctatcat
    40861 cacaaaaggt ggagatatag taaatgttgt tcctgctgat gttcatatgg aatcatatgt
    40921 tagagctaga actataaatg gtatgattga tgctaatgaa agaataaata aggctttgat
    40981 ggctggtgga atggcagttg gagctgatgt tgagataact gaaattcctg gatatcttcc
    41041 aatattacga tacaaaagtc ttgataattt atttaaaaac aatcttgaag atttaggtct
    41101 aaaaggaaaa gttattgatg gtggagattt tacaggctct tttgattttg gagatgtgtc
    41161 tcatataatg cctactcttc accctatgat tggtggtgta aaaggagctc ttcatacaag
    41221 agattttgaa atagttgatg aagatttagc ttacataatt cctgctaaag caatggcact
    41281 tacagttgta gaccttcttt ttgataatgc taaggatgct aaagatatat tagatacctt
    41341 tactccagtt atgactaaag aagaatatct aacattctta gaaaaacacg ataaaacttt
    41401 ataagtctat ttttataata gggtggtatc tactgtaaaa gttataccac tctttgtttt
    41461 tgtaacagta tacttctttt cttttagttt ttagattaaa ctaagatata ttatcctcct
    41521 taaaactttt agagctacgt tgaaatatca gtagaactat gctatattct ttctaattaa
    41581 tataaatttc tattctaatt caaggtggtg aaaaaatgtt taatgcagga aatcgaatta
    41641 taaatacatg gttgtaccct attcaagatg gctttgtctt gattgatact ggatatgaaa
    41701 atggttttaa acatttcaaa aaacgaattt ctaaattcaa cataaaaatc aaagatattc
    41761 gatacatatt tctaactcat gctcatgatg accatgttgg ttttctaaat cagattctag
    41821 acgaatctcc taatacaaag attgttatgc atgataaagc tttagaaatt ctatataaag
    41881 gtcaaaactc ttttattggt ggttgtacca gccgtactgc ctttctcttt tgccaaatta
    41941 tgaaactatt tggaaaaggt gagcatagtt tttctccact taaacaagaa tttgaaaaaa
    42001 ggtgtattct tgtgtctgac aagaatcgta aagaaattga aaaagaacta aatggaaaaa
    42061 ttatagatac accaggacat actgctgatt caatttcact actaattgat gatggtgttt
    42121 tattttgtgg agattctgca atgaatggat ttccaagtac tcacaaaatt accatatttg
    42181 cagaaagcaa actagaattt attcaatctt ggaaaacaat catcaataca aaacctaaaa
    42241 tgatttatcc tggacatgga aaaccatttg aatacagtaa attagaacaa aatctaatct
    42301 atgtaaataa aataaattta tatcctctac ataattcaaa agatattcac agatttttgt
    42361 agaaaataca aagtaaattt atttgattaa ttaaaaaatc taacacttta ctacgctact
    42421 tattttaata acttaatctt ccactagctt ttcataaaat tttgttttaa aattaatttt
    42481 aaagtgcata tgttatttaa atacattaga taaaattcgt cattaatcta cttaaagcat
    42541 atttaactaa caccactcaa ccagaaaatt cagaaaactg cattgacaca ggaaaaagtt
    42601 tattttatac tttttttagg acatattgtg gacacgtctt aaatcgacaa gcagaattta
    42661 aattgtaaga ttaaccacaa tacttatttt ttaattatat ttttaaacat tagggaggat
    42721 attatgaatg ataacgtagt taagaagaaa aacttttttg acaggacatt aaacagaata
    42781 gaaactgttg gtaataaact accagaccca gttacaatat ttttagggtt atgtgtactt
    42841 ttacttatac tatctagtct agttggttct atgggtatat cagtagttca tcctggtact
    42901 ggagaaacta taaccgctgt aaatttattg actgtagaac agcttcaaat tcttttagga
    42961 aacatagtca gcaatttcca agggtttgct cctttgggtc tagttctagt aactatgata
    43021 ggtgctggtg tatgtgacaa aacaggactt atgacagcaa caatcaaagc atcagtaagc
    43081 aagattcctg aaacaagagt aactcttgtt gtaatgacta taggtatgct tgccaatata
    43141 gcttcagatg caggtacaat attatttcca ccattagctg cacttgtata tcttggtgtt
    43201 ggtagacatc cacttatagg tttattttct ggatatgcag cagtctgtct aggatttgcc
    43261 gcaaacatta tgataagtgt caatgatata cttgctgcaa gttttacagt tccagcagct
    43321 caaatgattg attctggatt tcaagccaat gcaacaatga acttattttt tatgatagca
    43381 tcaacttttg ttctaatact tttggcaaca tgggtaacag aaaaaattgt agctcctaga
    43441 tttggtaaat atgaaggaaa tgcagaacta gatgttgatg gaagtcttag tgatgtagaa
    43501 aagaaagggc ttaaaaaagc tggtataagt atattaatat atgtggctat tatagtagct
    43561 cttagtgtaa tcggtaaaaa accattctta gctgacccag aaacaggaga tttactatca
    43621 aacaatgctc ctcttatgaa aggtatggta cctataatag cactagcatt tttcatacct
    43681 ggtgttgtat acggaaaaac tataggtaaa ataaagaagg ataaggatgt agtctctata
    43741 atggctagtg caatgagtga tatgggtgga tatataatac tagcatttgc tgcttcacaa
    43801 tttttgcaat tattcacaaa tagtaattta ggtctgatat tagctgtaaa gggcggagaa
    43861 tttttaaaat cagcaggaat aaatggacct ttattgttga taggatttat tatattatca
    43921 tgttttataa atttatttat aggtagtgca tctgcaaagt gggctattct tgcacctata
    43981 tttgtaccaa tgtttatgat ggttggatac aatccagcac ttactcagat ggcttatcgt
    44041 ataggtgatt gtataacaaa tccacttagc ccattatttc cgtacttccc tattatacta
    44101 gcattcacaa gaaaatacga taaagatgct ggtatgggta ctgtaattgc taatatgata
    44161 ccatactcta tatcattctt aatagtgtgg actatactat taatattgtt tatggtattt
    44221 aatattccac taggacctgg aatacttcct tcatatagca tgtaatttat ttaaatcttt
    44281 ctaaaaggag aaataattta tgttagataa aaatagactt ataaataatt ttatggatat
    44341 ggttagaatt gatagcccat ctaatcaaga attagaaatg tctaagtggt tggtaaacta
    44401 cttaaaagaa agaaatatag acgccattat agatgatgca ggtaaaaaat atggtggtaa
    44461 tacaggtaat gtcattgcct atataaaagg tgaagaagga agtagaccac tttgtttatg
    44521 tgcacacatg gaccaagttc aaccttgttt aggtgtaaaa ccaatactag atggtaatgt
    44581 agttagaagt gatggaacta ctacacttgg tgcagatgat aaagctggaa tagctgctat
    44641 acttgaagct ttagaacatg ttattacaga aaaaatacca catagagata tttatctttg
    44701 ttttacaatc tgtgaggaag ctggtatgca tggcgtcaaa aactttaatc ctgacaactt
    44761 gccttgtaaa gatatggtga ttttagattc tggtggagct attggttcga tagcatacaa
    44821 agctcctgct caacaatcta taaaaatctc ttttcatgga aagaaagcac atgctggtat
    44881 agaacctgaa aaagggttaa atgctatatt agtagcctct catgcaataa gcaacatgca
    44941 cataggcaga atagattcat tgactacttc aaatataggt aaaatagaag gtggtggagc
    45001 tacaaacata gtaactgata aggttacatt gactgctgaa attaggtctc atatacctga
    45061 aactttagag tatgagctta atcatatgga aaaatgttgt aaggatgctg catctaaatt
    45121 taatactact tatacttttg aacataatat gtcttaccct agttttgaac ttagtagaga
    45181 tagtcacgtt ttcaagttaa gtgaagaagc aataagacaa gttggtgttg ttccaaatcc
    45241 aatggtaatt ggtggtggaa gtgacgcaaa tatattagct aatcttggat ataattgtgc
    45301 tatattaagt ctaggtatgt atgatgtaca cacagttaat gaatatgtaa atatagacga
    45361 attatatgat actgccaaaa tagtttatca tatgattaaa ttataaatat cataatagaa
    45421 ctctttaaat tataaaaata aaaacctatg tatcttcaac tttataagtt atatattaga
    45481 gttgttttac ataggtttta gtatttttat aataatttgt tctaattttt ttcataataa
    45541 gctttaatac tttatatagt aatttgttgt agaattttat acaatataat gatttataca
    45601 gttttataaa atatcttaaa acagtatttc agtaatattt tttggtcttc ctccaccaat
    45661 actagtctct tttgcacata ttcgacctaa acctgcattt actattttat ttaatatacg
    45721 tcttgcactt ctttcactta catttagata ctctgctaac tcttttgaat cataaatctt
    45781 actttttcta ttctcattta tagcaataat ttttgcaact gactcacaac tcattccagt
    45841 ctttttagat atttcgatta ccttttcatc agatagcatc aaagaataat ttaattcatt
    45901 ttcggaagct aaaggtcctc ttatgttttc atcttcatct accaaatata taccaatttc
    45961 ttttgaatct atggagtgtc ttaatgcttt atgtgcatta ttttcagcct gatacgcagt
    46021 tactccagta ccaattccta tactaagaga aaagcccatg tcttttatct ctctctttag
    46081 attgaatagc ttatcataat tatactcatt atcagctacc cctttatgaa caaatacaac
    46141 ataatcattt cttccaagta aaaataagga accttgaatg cttcttacat agtttactac
    46201 aagcttatcc atatctgatt tttttatcat gtcagaataa tagttatcta tcttctcttt
    46261 ataatctatt aaactcaata tttcaacagc aatttgagaa aatcttgcct tacttagagc
    46321 atattcactc tttaccttgt cataactttc tttgattaat tgtatagtag cttgtaaacg
    46381 aaatacagga tatcctcttt cttttaattc attatatact gcaccaaaac cagtcaacat
    46441 tatatccgtc ttattttctt caaataattt tatatgccat tctacaagtt cagattctcc
    46501 tacttccaaa gaaaatggat tacaaaatac actcgttgct ttaatgtcaa attctttaag
    46561 tgcatcttcc acaacatatc tatcaactac atctatactg aatttatcta attttatatt
    46621 tgcacttttt attgcccaaa atgccttcat tatactcgtc ccacttctcg gtacaaatga
    46681 atgtggctta cttatctcat acttagattt tataaattca taaactgcac atcctgtaaa
    46741 tataatagca tcacattcgt cttcacattt agaaataact tctattgtat ctactacttt
    46801 ttctcttaca tatattttag tctttaaatc actgtctatc tgttttaaat catcctttat
    46861 cttaaaccca gagtctgaag gacctataat acccactatc ataaacttcg ctcctaatcc
    46921 ataaatatta tactttttaa aaattatatc ctcactattt tctacttcta tttaattatt
    46981 ttgaactact ccatcctacc tacatttaga atggaataat tagcttattt ttgataaagt
    47041 aacttctcaa aatcagcact tggaataggc ttacaataat aataaccttg aattaccaca
    47101 caatctaaac tcactaaaaa ttcagcttgt tcttttgtct ctacaccttc tgccactaca
    47161 tccatatcta aatcttttgc catggctata gtgtgcttta aaactatttt ttctctgtct
    47221 gtgattcctt cctcattaaa gaaaccacaa tctatcttta atgtatcaat attaatattt
    47281 tttaacatat ttaatgaaga atatgctgaa ccaaagtcat ccatatttaa aacaatacca
    47341 tccattctaa ggtcggctat tatcttagta aataatttta tatttttaat aaataaactt
    47401 tctgtaagct ctaattctaa aaaagatttt ggtatactat atttatttat taaacttaaa
    47461 acaatttctt taaaatgtgg attatgcaaa tgtaatcttg atacatttac agagatagga
    47521 actggctctt cacctaaatc tatccatttt ctaatcaaag aacatacttg ttcccataca
    47581 tacatatcta atctgataat aaacccattc ttctcaaata gtgaaataaa tttaattgga
    47641 ggtattatac ctttttctgg atgaacccat ctaaccaatg cttctgcacc aacaatttta
    47701 tttgcattaa gattatactt aggttgtaaa tatacatgga attgcccatt ttcaagtgcg
    47761 tattccattt ccctttccat ctgtttttct tcaagtgcat catttcttat agtatcatca
    47821 taaaatatat agttgttgat actttgtttc tttacttttt tgtgagcata tcctgccatt
    47881 tcacacgcat ttgtaattgg aatatgatta tccaaaattc tataaactcc aaaacatggt
    47941 actaacatat attcaaattt ttttctattt aagctatttt taatcttata aattacctta
    48001 tgaatatctc tatcagagta actctcataa aaaatgcaaa atacatctgc atacatacga
    48061 caacatatac cattagaacc aatgtttttt tgaatcaact ctgcaacata tattaaagct
    48121 ttatctcctc ctctaactcc ataaatgtca ttaattacct tgaacttatc tatatccatc
    48181 attataattg cataacgctt gtcaggatat cttttaagca ttaaacttat ttgttcacaa
    48241 aaactttcat aattgtatag tcctgttaat gtatcttttt ggtttttatg tagttgcata
    48301 agataatcta tattttttat agcaactaaa atatgtgttt gtttctcacc taacttattt
    48361 ttactactaa tctttaacat ataaattaat gcccaatagt actttttatc tattcctaaa
    48421 cgtctatact caaaatttag ttctaaatcg tcttgcttgc tattattaag actatcaaga
    48481 gattttagaa ggtggttatc attaaataat attatatcat caggatgaat taaatgtttt
    48541 tttatgtatg aaaaagtttt ttcaagattt tcctttatag gaaaactaaa aatattgctc
    48601 tctgattgat aaattggagt aattaaattc gtatcaatgc aaatagataa aattatatca
    48661 tatgtattat aagctaattt ttcataagcc ttagaatacc cttttatcat taaatcatca
    48721 aaataaacct tatcaactaa ctctttatgt ttaaataaaa tattattcgt cgaatcagct
    48781 aagtcactaa ttactttcat ttcattattt ctaagtttgt tcttattata atacctcttc
    48841 ttatcttggt acattctctc atcagcaact ttaattagct tatgtatatc aatattatcg
    48901 tcactccaaa tactgcccac tgctacacta tatatcatct catccttaaa ttcttcactt
    48961 aaccaactca ctaattcttc aaatagttgt tttgatatat cttcacataa tacaacaaac
    49021 tcatctcctc caatacgata aacattataa gaatgaaagt atttttttat tattacacat
    49081 gctgacacaa tagcagaatc gccattagca tgtccaaagt tatcattaat atcttttaac
    49141 ccatttatat ctacaaatac aactcctatg gatttaactt ttcctttttg aaaattatct
    49201 atatacttaa tatatttatt tctattatat gcaccagtca agaagtcata gtagctcata
    49261 taatttaatt tattttctac ttttcttttt ttcatctcag ttaataaaaa ataagatagg
    49321 gatgtcaaaa gagaggtatc tccaacagct ttatgtggat tatcaactcc aataaaccct
    49381 actactttat catcttgttt tagtgaagat gtcattaaac tttcaatttt ttgaggtttt
    49441 aaaatttcat attctattgg ctgttcttct tttatatttt ctatatcatc aattattaca
    49501 aaaccttttt gttcgaataa taaaaaccaa ttctttaatt cattaatatc aagatttgtt
    49561 agtgcctgtt tttgttcatc aacaccttca ttgcaccatt catatgtatt agttactttt
    49621 gttctatctt ctgatacttc aaaaacatat gctctatcag ctttatgaaa tttaccaata
    49681 ttggttaata caagatttac agcatcagaa aaattagatt ctgttataag atgtttaata
    49741 catccaacta ataactcctt tatctctaat ttttctgcta ttgacttact tatatcttcc
    49801 ttttctgtaa tatcaagagc tatttctaat cgtgcatgtt tgccctccca tttaattaac
    49861 ttgtccttta atataaaatg tctatttgtt cgagtattcg tattttccca tatgtaatat
    49921 tcatcttctt ttaaaataga gtttgtacaa aattcgcagg gagaatcttt cccctgtaat
    49981 gctttataac attttaaatg ggatatatca ttaagtccca ttgccttttt cccaatttca
    50041 ttaatatata aaagttcata tgtatctaaa gaagaaacat atacaatttc atccaactca
    50101 tctattaaat ttattacatc catttatata tatctccttt tattcatttt ccagattgaa
    50161 tttttaaagc ttttaaaatt ttgctatata tttggcaatg atattatact tctttatgtt
    50221 ttaaaatagt accttaatga ggttctattt tttagtaaat agaattatta ttgtgtcaat
    50281 cgtacaatag agttttgtaa tacctaattt aatatattta taattaatta tctcattatg
    50341 tgtttataat taattctcat ataaatgaca aatctccacc tcaattaagt attccaatca
    50401 tatatgaaga taatacttca ttcatttaaa aatataaaac tattatatat taaaatttct
    50461 tcaataataa attatctaga ttgttacttt gatatattat ataatatcac aaaggctgat
    50521 ataaaaagac aaaaagggca atttttttat atcaaaaaaa atgttaaatt gatattatcc
    50581 atatagatgc tatatttaac aattctactt acttccctta cacttattag catatagtta
    50641 ttaagagttt gttgcaaaac aacacttagc acactttaaa aagtgtggtc taataagtaa
    50701 tcaacgaaga agacataata aagcataaaa tatgatatac ttttccacgc catcaaaact
    50761 gaggatttta ttattaatat atgctaaaat atgcttaata aattaaattt taatgggaga
    50821 atagttatga aaatatttag tttcttgttg tttgtgatag gaatatgttt atgtattgct
    50881 ggatttatag gacattaccc agtatttgtt ggtacaacat tgagttttct tgcaattgca
    50941 atgatagctg taagacaagt caaaactaag aaaaaatgaa ataggcaaat cctaacttta
    51001 caagttaata ttacacaagt aattagtcta aaattggata ctttttctat tacagaaata
    51061 catcatccac tttgtatttt cttaaaaaaa taaaatagct atgtaaattc aagattagat
    51121 agattaactc tctatccatt tcattgtaaa atatacatag ctatttgtat tcgatttaaa
    51181 atacttagat tttcttacat aacaaataat ttctatctga ttattactca tattcattta
    51241 gtaaccactc agcaactaat catttagtat ctattcatcc gccatacggt aacctacccc
    51301 aacctcagtc aaaatatatt ctggctgagc aggattcttt tcaattttcc ttcttatact
    51361 tgccataaaa actctaagcg actgattttc actttcatag taatttcccc aaactttatc
    51421 tattataaac ttatgggtta agactcttcc tgaatactta gacatcaact caataatctt
    51481 atattcgatt ggagttaaat gtatttcttc accattaatt gacacaatat gattttcata
    51541 atctatcttt aggtttttaa cttcaaaaga ctttggagca tcattattta tattatcctg
    51601 ttgagcttta tttctaagag ccacacgtac tctagccaaa agctctgtta cactaaaagg
    51661 tttagttaaa taatcgtctg ctcctccatc taaagcttcc accttatccc tctcatgctc
    51721 tctcgctgaa acaacaatta ttttattagt ctttgcacat gccctaactc gtcctattac
    51781 ctcaatccca tcaatatctg gtagtcctaa atccaaaatt atcaaatctg ggtcaagaga
    51841 atgtataagt gatatagctg ttcctccatt atctgtctct acacacctat aattttgagt
    51901 ttcaagagat acttttataa atttacaaat tggcttatca tcttctacaa ttagaactaa
    51961 tggctttatt tccactcgtc tttcccctcc tcatcataca taggtatgct aaatctaaat
    52021 gtagctcctc cagaaggatt attaaaggcg acaatttctc caccatgagc ttctactata
    52081 gatttacata tctccaatcc aagaccaata cctcttcttt caagatttct agacttattc
    52141 ttagtataaa acctattaaa aatattcccg atatcttcat ttggtattcc ttttccatta
    52201 tctgcaactt caaatagtac ttgatttgac tcttttttta cagttagttc tatcttagaa
    52261 tttttagatg tatgccttat agcattatca attagattta ctaacacttg ttctattaat
    52321 aatacatcca catgaacgat tattatctca tctggaatat ttgtttttat gtctctacta
    52381 ttggcaaact ttttgactct taatatagat tcagctatta tttcatcaac aacctctggt
    52441 cgtttttcaa tatccaattt tccttcatct attctggtca tactaagtat attctcaaca
    52501 gaatgagtta gccaactcgt atcttcatat atatttttca ataattctac tctagtttct
    52561 ttatctatca catcatcatt ttcaagtatt gttgaagatg aacctaatat acttgtaagt
    52621 ggagttctta aatcatgaga aattgaccta agcaagtttc ctcttaatct ctctctctct
    52681 gcctcaagat ttgctttttt atttttctca aataaatttt ctctatcaat tgccaacgca
    52741 acctgtgttg aaactgcttc taatagtatc ttttcatttt ctgtaataat atctatctta
    52801 aaacacgcaa cacctataac accaagaaca tttgattctt gaccttttat agggaaataa
    52861 tatgcactgc tgtttttaca atattttgtc ccaattccaa ctgggttacc tgtcctaaaa
    52921 gcttctttta tagcttcctt ttctaatact gaatggaata tattctcact tccatcattg
    52981 ttacaaatat aaatacttgg cttacctaga ttattattct catctgcaat agtcactatt
    53041 acagttctat ttaatatatt tactaaatta gacccacaaa attcaataat ttgattttta
    53101 ttttttgctt taagcaacgt tttgttattc tcatagagca atcttatcat tttttctctc
    53161 ttatgagaaa gtctcacttc tctttttatc ctagaagtta aagtacttgt tataattgct
    53221 gccattagca taatacaaaa ggtcatagga taatcttttc tataagctaa aaaagtatat
    53281 aatggatgcg taaagaaaaa attaaatgat agcactccta ttactgaaga aagtattcca
    53341 aaaaaatatc cataggttat acttgtagca aatagtacac ctaatatgta tactagtata
    53401 atattagatt cattaaaacc taccttttca aaaactactg atattccagt agcaattgtc
    53461 ataaccaata tagtttttat gaagtcctta ataattatct ttttactgag atagccatct
    53521 tcatcataat catactcttt taagtcttca tgttgtagtc gttccttttt catttactac
    53581 aattcctcct tattgttact gtttaaattg gctctatttt atataataca attttatcat
    53641 aataaattca atcaatacat ttaattaatg tacttaaatt actaaaaggt gctgtcttaa
    53701 aatgaaaata aatctctatt tcaaaacagc acctatatat ttaaattact aatttagttt
    53761 ttacatactt aaatcaatag ttttattcaa tttcataaat tataatactt caattataaa
    53821 acatttctat ccaacaagca taaatataaa gtgggcacat atacctgcac ataaaccacc
    53881 tatagtatgc gaaagtatcg ctttaccagc caatggtctt gcatctaaag catccatcat
    53941 acctatgtgt gtacttaaat atccactcca acacataccc attgctgtga atactgctat
    54001 atcattagga gttatagcgc ctgattttat aaactctggt actaaagata ttgctgctcc
    54061 aacagctcct aatgcagtta cagggaatgc tattgcctct ggtgatgtga atccaaataa
    54121 tggttctatt atgaagctaa tcttatcacc aatagcaggt aataatttta taccttcata
    54181 tgcagctcct gtgtaaacag cttgacctgt agcagggtct gtagatggtc caaaagttaa
    54241 taacataact aatgtacaaa ctactaaaac tcctggtatt atagccatac ccatttcaac
    54301 acctaactta ccaccttcaa gtatagcatc caatgttctt tggaatacat taccatctct
    54361 tatttctcta aactcttcaa gttttgcacc tttatcattt atttgctttt cagtatcatt
    54421 tttagggtca taattataat actttttagt aaaagtcaac ataattctaa cactaataat
    54481 actacctatt attgccccaa cattacctat tatagctggt agtacatatt ctttaccttg
    54541 agctatcata aaagttgtta caataagtcc cataccaaag gctgttccta aattacataa
    54601 agcaggtact tgatacttct taaaatattg tgtaaatgtt ttgtctttag caaatggtat
    54661 aatagctgga ttatcagata aatatgttgc aactactcct gctatactag caccaggtaa
    54721 gccccatata ggtctcataa gacccttaaa tatcttatta actaaagata ttactccaaa
    54781 ctctgaaagc aacgcactaa gtgcacctgc aagaactgcc atagccatta ttaaaaatac
    54841 tgtgtctaat aaaagcgcat gtgctgtact cattattact ttaaacatca ttcctgcacc
    54901 catgatgctt cctacatatc caaatcctac cgccaatagt acaataaata cgaaggtttc
    54961 catacttact gccttgactc ttctcttatc cgacagcttt tccatcttga aatcttcccc
    55021 cttttaattt taaattaagg ttcctaacat tggtacaaat tttttggtac aaactgagtt
    55081 ctaaaactta cactcgtaat gcaagttgtc agttttttga aataaaaaaa aattacgttt
    55141 atagaataag aatatcctct ttaatttgga tactcttatt ctattttatg ttattatatt
    55201 atcatccttt tgtttaaaag tcaatttttt ctatttttgt tgtttaattt tatttatact
    55261 ctttttatat ttttcatttt cttttatctt tgtaagtact ccatataagt tgacttttct
    55321 aaatacagta gttataaata ccaatcctaa tgcaattgaa cttgcacacg ataatgtact
    55381 caccccatat tctacagctt ttatataatt attggtcaaa acagaataca tagtattata
    55441 tattccataa cctggtacta atattattaa acagcaaaca cttaatgtag ttgctggagt
    55501 catataaatt cttgcacata actcacaata tatcgcaaaa caaactgtag ctataaaaaa
    55561 acataagttt tctgttactc ccatatttaa aagaaattta tacgaaaacc aagctatact
    55621 tccaccgatt cctgctagta ttaagttttt tcctttcata ttaaatagca ctccaaagct
    55681 taaggcagta aaaaaagctg ctattacttc tataaacaaa tgcacttata tcccccctaa
    55741 cttgaagtac aatccaagta ccaagaacat tcctactgct atagatacac ctacaaaaaa
    55801 tacttctgct aattttgcaa gaccagaaat caactgtcca tctaacaaat ctcttaatgc
    55861 attagtcaaa gcaagcccgg gaactaagag cattattgct cctgctatta aatggtctaa
    55921 cgtctttatg aatccgagct ttaaaattaa aattgaacaa gaagctataa taactgctcc
    55981 aattgcattt acaaaaaagt cattaaggga agatttactt aaatacataa taacaaactt
    56041 agtcagaatc ccaacagtaa atgcagctat aaattcttcc acatttcctc caaataatat
    56101 cgaaaaagtt gctgctgcaa tacctgcaaa aaatattgtt attaaatccg agtaatagtt
    56161 atcttcactt atttcatcta aaattttctc acataattca atatctaaat tatttatact
    56221 tattgctctt gataatgagt taacttcatg aaccaagttt aggtctactc ttcttgagct
    56281 tattctttta attgctgaat gtaatttccc atctacaaat acagatgcca ttacagctgt
    56341 tggagttgca aatacatcta cgtggtctat tccaaaagat tgacatattc tagctatcgt
    56401 ttcttcaact ctataggttt ctcctccact ttgcaacata gctttacctg catttgagga
    56461 aaagtttaaa attctatcta tatccacaaa acatatctcc cattctttta tagtattatt
    56521 catattttaa tacaagatat tatttattta aactatttta aataattcat atccaatatt
    56581 tacattttat gaaaaaactt gttttttatg cttgtcaaaa tcaaatttta agcttgttta
    56641 caccataaaa tggctgaatt aatatttgac aaacactttc aaccatttat ataaccttct
    56701 aaattgtata actttttact acaaataaaa ttatatctct tttaattctt tctcttgagt
    56761 atttagctta tagtaacttc atatttatat atgtacaata taaaacatta tttttatgat
    56821 acaaaatata atttgtaaat gtatatatgt attaac
//
LOCUS       DBGJFF010000007        56287 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.007, whole genome shotgun sequence.
ACCESSION   DBGJFF010000007 DBGJFF010000000
VERSION     DBGJFF010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 56287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56287
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.007"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(255..1064)
                     /locus_tag="ACOIAF_000530"
     CDS             complement(255..1064)
                     /locus_tag="ACOIAF_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGZ2667557.1"
                     /translation="MYYVYVNDINIAVYDLNPSAKKTVLFIHGWPLGHKIFEYQTNIL
                     PKLGYRTVSIDLRGFGKSDATSGGYTYSQLADDIYKVVHAIGLKDFTLVGFSMGGAIV
                     LRYMSLFNGYGVSKLVLAAAAAPSFVQRPPEFPYGMTREDVNKLIAQACTDRPEMVTD
                     FGEKVFASNPPESFRRWFNDIGFSASGIGTIGTAVSLRDEELFNDLKCVRVPTGIFHG
                     KLDEICPYEFAVFMNEKIEDSILYTFEYSGHAIFYDELKLFNQEFLQFLEE"
     gene            complement(1148..1681)
                     /locus_tag="ACOIAF_000531"
     CDS             complement(1148..1681)
                     /locus_tag="ACOIAF_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HGZ2667558.1"
                     /translation="MSDAHYDMHKRNGYSSDEPYPEIKVLGPNKYYAELLMDDYAGVS
                     SEFTSVNQYLYHNFDLDETHRELSEMWINISITEMLHMEILAKTIRLLGGNPVYRGST
                     SSCGAYWNGGFVCYGNSICNRLKLDLHLEHVAINNYYKDISLIEDPYIKAILNRIILD
                     EKLHVSLFEKAIEKYCK"
     gene            complement(1831..3180)
                     /locus_tag="ACOIAF_000532"
     CDS             complement(1831..3180)
                     /locus_tag="ACOIAF_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HGZ2667559.1"
                     /translation="MELKNFKDILFKKALSEGFEECEIYYYTGENLSINIYEGEVEKY
                     NLDKSFGLSFRGKVNGKIGYSYTEILDDKAVDMLIKNVKDGVNTIENEDVQFIYEGDK
                     HYNDVKTYSKELENLEADKLIDLALEMERETKAYSDKVVNLSKCTISYSVSSNGISNT
                     KGLNLSNKTNMLIGFVVPIIEDNGQKYDGIGYNIANSIEEIKPCEIAKLGVDNALSKV
                     GGKSIPSGKYKTILLNEAMVSLLSTFSGIFNADSAQKGLSLLKNREGDMIASPIVTIV
                     DDPLLENGMASTPFDDEGVATFKKEVVLNGKLITLLHNLKTANKAGVKTTGNGIKSSY
                     SSPISIYPTNFYIEKGDKSLDEIIKDIDEGLMVTSFAGLHSGANSVTGDFSLAAKGFY
                     IKEGKKVFPVEQITVAGNYFDLLKDIEAIGADLEFPMSSIGSPSVVIKELSVAGKDE"
     gene            complement(3192..4574)
                     /locus_tag="ACOIAF_000533"
     CDS             complement(3192..4574)
                     /locus_tag="ACOIAF_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HGZ2667560.1"
                     /translation="MLSKNIASKVLEKCLITGGDFAEIFEEDTINNSISLIDNKVENA
                     IGGRNYGIGIRIFKGLKSIYAYTSDNSLNSLLDVAYKAAVALGKLEDGKSVILNDSIK
                     INNIHNIKIYPNSIGNRDKVSVMKVAYKSAKEFSNDISQVSVSYLDKDQKILIANTEG
                     IYVEDRRIRTRLGISSIASKGNENQTGFEGPGGCKGFEIFEEIDPEYYAKESARVAHT
                     MLHAKNCPAGNMMVAIDNGFGGVIFHEACGHSLEATSVAKGNSVFADKLGQQIASTKI
                     TAIDDGTIPNYWGSMNIDDEGNPTQKNILIENGILKSYMIDKLNGRRMGMNPTGSSRR
                     QNYKFAPTSRMTNTYIAAGEDRPEDIIKSIPDGLYAKKMGGGSVNPVTGEFNFAVSEG
                     YLIKNGEIQEPVRGASLIGKGSDILMNIDMVGNNVKQAQGMCGSSSGSIPTNVGQPMI
                     RVKEITVGGR"
     gene            4818..6032
                     /locus_tag="ACOIAF_000534"
     CDS             4818..6032
                     /locus_tag="ACOIAF_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HGZ2667561.1"
                     /translation="MNQEYVKGNPIFTNINKVPRQFPYLTDDIDTDVIIVGGGVTGCI
                     CAYYLAKNNIKSVILEKGRIAHGSTSVTTSLLQYELDDNLIDLTEVMTLKDALKAYNL
                     CTSALEELDTFIELYGNKCDYAKRDTLLYTANKLEVKAIKEEYNLRKENGFDVEYIDE
                     STNPFSFDLKSGLIAKNGGRELDPYKYSHHLIDVSLKNGLQVYENTEVKKVDLSNDKV
                     TAEVSYGYKVHGKKLIVATGYNTSLFTKRNFATKSNTFNIATKPLKNIASWKNNILIR
                     DNCDPYNYLRTTKDNRLIIGGEDVSFDDIENETLANEKYDILEQRLKSMFKDIKDIEV
                     EYKYCGCFASTLDNLGFIGPDNKNNNLWYCLGYGANGILFAILGGIMLSELYLGKQNK
                     NMKLFKVDRFDK"
     gene            6209..8122
                     /locus_tag="ACOIAF_000535"
     CDS             6209..8122
                     /locus_tag="ACOIAF_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HGZ2667562.1"
                     /translation="MKYGGIIMKEILVIAPTKGTYEKSIHIVKKNKYTNIDVVFGNLK
                     EGIPLAEKSINHGTRIIISRGGTYNMLKATYNIPIVEIKVDAYDIIKSYKEVKNSNEP
                     FGIIGFNNVIYGFDIIEEILNKKITMIEIEKEEEIYDAIEKYRKKGINTYIGDTTVAH
                     IVKRLNCKGILIESREENILRAIQQAEQILEATKDEQKRRLQIEAMTDFVHDGIITVD
                     KNFKITLFNKSAEKIFGIKKENALYHNVVDVIPNTVLPQVVETMEPQIGEIQNIGSTK
                     IITNRVPIIVDGQIQGAVSTFQDSKEISSFEHTIRRSLLSKGLSAKYTFNDIIHASEK
                     TKDCIELAKKYACYDTPMIITGESGVGKELFCQSIHNYSKRKNAPFVAVNCAAIPPSL
                     IESEFFGYEEGSFTGARKKGKAGVFELAHEGTIFLDEISEIPLELQGRLLRVLQEKQV
                     MRIGGDKVIPIDVKIICASNKDLKNMMRNGLFRQDLYFRINILTLYLPSLKERKEDII
                     KLSEFFIHKYSSKYNKTPLIITPNIKKALLEREYEGNIRELEGMMERAVIVESFDSIL
                     LEKLSNEYTNNIVDKDNSDFECSNLDLKTLEKKYIYKIYNENNRNTTKTCEILKITRS
                     TLWRKLKEIEDDV"
     gene            8393..9367
                     /locus_tag="ACOIAF_000536"
     CDS             8393..9367
                     /locus_tag="ACOIAF_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HGZ2667563.1"
                     /translation="MHILKSVKKIPGGLMVCPLLFGALFNTVCPQALEIGGFTTSLFK
                     TGAMSILALFCLCNGAQINIRQAGKPLTKGIVLTATKFIIGAVLGIIVSKFIGPKGII
                     GITPLAIVATMTNSNGGLFAALAGEYGDSTDVGAISILSLNDGPFFTMLAFGVTGLAN
                     VPIVALFAALIPILLGFILGNLDEDIREFLAPGTTLLIPFFAFPLGAALNFNQIITAG
                     LPGVVLGLGVTIITGMGGYFVMKLIRAEHPAVGAATGTTAGNAAGTPEALAAIDPTLA
                     TIAAVSTVQVAAAIIVTAICCPMLVSYLDKREKKKKSIFKTNKNIEVN"
     gene            9388..9663
                     /locus_tag="ACOIAF_000537"
     CDS             9388..9663
                     /locus_tag="ACOIAF_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HGZ2667564.1"
                     /translation="MNAIIHANEKDNLVTCVRPLVKGEKVSIDGKSYIVNDNIPVFHK
                     MATEDVKKGDVVYKYGEIIGIATVDIKTGDYVHVHNIESTRGRGDKK"
     gene            9676..10839
                     /locus_tag="ACOIAF_000538"
     CDS             9676..10839
                     /locus_tag="ACOIAF_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HGZ2667565.1"
                     /translation="MKLMGYLRENGQFGIRNHVLIIPTSVCSSETATRIASLVPGAIA
                     IPHQHGCCQIGSDIKLTAKTLIGFGKNPNVAAVLVVGLGCDGIQAKELASEIATTGKK
                     VDYVVIQECGGTLKTVSKGAEIVSKMAREVSKEVRVEFGMSEITLALECGGSDPTSGI
                     ASNPSIGVASNLLVDEGGSSILSETTEVIGAEHLLATRFEDEEMKDKFLKFVSDVEKR
                     AIAMGEDLRSGQPTPGNKAGGLSTIEEKSLGCMYKAGNKPFKGALEYADIVPPDKKGL
                     YFMDTPGQDIDSITGMVAGGAQIVIFSTGRGTPTGSPISPVIKITGNSDTYNKMPDNI
                     DINAGRIITDGAKIADIGQEIFNEIIEVCNGKHTKAESLGHREFGIYRISSTF"
     gene            11176..13455
                     /locus_tag="ACOIAF_000539"
     CDS             11176..13455
                     /locus_tag="ACOIAF_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HGZ2667566.1"
                     /translation="MNFENTKNKKIIATLLLTLLLIVSLTCVTFIMNLNKVTHNETER
                     YLVEVSQSISTTINVKCKSIFRTLYSMGDTYFEINDSEKEVNRYLSKKLKLFEFDWLG
                     IIDSNGNFVSALKDTGNVKGQDFYRKALQGKETITPVKSQSGTYGILYSVPLYNGDKI
                     VGAVAAWNTIDTMRNLLSVESFHGEGFSQIIDQDGNFIVSSANKNAPKNVVNFFDMIK
                     HQGKQVDKNKLEEMKINLKKNKTGSMYYTLNDGISKAMHYLPLQEENLYLLSIVPTKT
                     ASAQFDLLLKKAIFINILIVILFLLLIILIMFINQKNRAQLIKIAYVDSVTKGYSKAR
                     FDIEAKKIIQSSSSESYSLVSINIQKFKLINDSFGSEAGDKTLKYIHDVISNYLEPGE
                     LLCRVSNDQFNVLIKTIPQKEILKYIENITNDINSFNKNEECKYFIALSIGVYKIDDP
                     NLPLVSIQDRANVARKNNKGNIGNHPLYTCVFYSDLERLKMIKEKEMENRMEEALNNN
                     EFIVYLQPKISLKDNTIVGAEALVRWQDPNKGLIPPDEFIPFFEKNGFITKLDHYVFE
                     KVCSMIRKWIDNGETPIPVSVNFSRANLNNIDFLEKYRMLRNKYNIPAKLLEIELTET
                     LVFENLQKLINIIDQIHEEGFQCSLDDFGSGYSSLNMLKEIKVDTLKLDRAFFSSPNA
                     DNIAENHVIESIVNLAKKLNMNSISEGVETILQMDYLKKINCDMIQGYVFSKPLPQEI
                     FEKMTFGKKLEETSMDSSV"
     gene            complement(13550..14890)
                     /locus_tag="ACOIAF_000540"
     CDS             complement(13550..14890)
                     /locus_tag="ACOIAF_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HGZ2667567.1"
                     /translation="MENQEALRHEKIWILLFRYSIPAIIAMMVTSLYNVVDRAFIGSM
                     EGIGSIAIAGLGVTMPVFTLIIAFGMLVSVGASTRLSIKLGERNREEAEKILGNALTL
                     SIIISLIITILGLVFLEDILFILGASKDSIFYAKDYMSVILVGSIFNLVAFSLNNAIR
                     AEGNPKLAARTMIVGCVLNLILDPIFIFVFNLGIKGAAIATVLCQIVVFFWVIHYFIR
                     GKSNLKLKKCNLKLNKDIVKKVFAIGMTPFAMEVAISITHMFTNNSLKVYGGDLAIGA
                     MTALTSILLMFMMPVFGLNQGMQTIISYNYGAKQYERAKKTLILSIIVSVVILSFGFL
                     VVQVFPEVFVGIFNKDSDLMDIAVRGISLNLITLPIMGISIVGPVYFQCISKVKHSMF
                     LTLLRQFILFIPLIIVLPIQFNLDGVWLAQPVADFIAMIIVLLFLKREFKTSRV"
     gene            complement(14902..15684)
                     /locus_tag="ACOIAF_000541"
     CDS             complement(14902..15684)
                     /locus_tag="ACOIAF_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2667568.1"
                     /translation="MNCINTKNLNVSYGNTDIIKNLNLNIPKGKITTIIGANGCGKST
                     ILKTIGRIISPNSGKIYINGEDIYKKKSKEIAKDMAVLPQSPQAPSGLTVSELISYGR
                     FPHKSGFGNSSKEDREIVNWSLEVTGIAEFKDRNMEDLSGGQRQRAWIAMALAQETDI
                     LLLDEPTTYLDLAHQLEILKLLDELNKKQGRTIVMVIHELNNAARFADHMIGIKKGKI
                     VCEGDAYDVMTKDNLKELFNIDAEIATDPRTNKPICITYDMV"
     gene            complement(15699..16721)
                     /locus_tag="ACOIAF_000542"
     CDS             complement(15699..16721)
                     /locus_tag="ACOIAF_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HGZ2667569.1"
                     /translation="MKNIIKINSKTLLVISILMVLIFITFLLSLNMGSFSIEPMDVIK
                     TFLGQGQRNHEIAIFKLRLPRIVIALLVGSALSTAGVILQGVTKNDLADSGILGINSG
                     AALFVVVYIYLMNGNVYEGMSNLTVFTMPIVALIGALFGAFLIYSLAWKGGINSSRLL
                     LIGIGVNIAFTSILTIFQLKFTTQEFNRVMAWTSGSIWGTSWKYVIAVLPFIVIFMGI
                     TIYKSKYIDVLNLGDEVSISLGVDVEKQRRRLIIYAVILSGVSTAVAGSISFLGLIAP
                     HIGRKIIGPKHKRLIPVSALIGTLLLLIADTISRNLLAPIEIPVGIVVSIIGVPYFIY
                     LMLVND"
     gene            complement(16727..17740)
                     /locus_tag="ACOIAF_000543"
     CDS             complement(16727..17740)
                     /locus_tag="ACOIAF_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HGZ2667570.1"
                     /translation="MTKLSKKNITYLLILVSIVVLVFGIVLSITIGAKDMNLSTVIDS
                     LIKMEDGINMRIVKDVRLPRAIAAALVGGFLAVSGAIMQGITRNPIAEPSVIGITQGA
                     TFAISISLVLQKKLPQLIHGSFSVMMFAFIGASISGLFIYFISSKSRGRVNNVKLALA
                     GVALGTLLISLASAISMYFNLSQQLSFWISGGLVGVKWEGIKLLFVAGGIGFILAIIM
                     APRITILSLGEEVAIGLGQKTNFVRFICIVLVILMTGASVSVSGNIIFIGLIVPQIAK
                     GIVGSDYKYIIPSSLVLGAVLLVYTDILSRMINPPYETPVGSITALIGVPIFIYLVRK
                     GEK"
     gene            complement(17868..18806)
                     /locus_tag="ACOIAF_000544"
     CDS             complement(17868..18806)
                     /locus_tag="ACOIAF_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGZ2667571.1"
                     /translation="MKKIKSLAIFISIITTLVLVTACSDKNTEDKDKSETRVVQSVKG
                     EVKIPSNPKKIVDISGSSEELLLAGYKPVATANVDSYETDKLPSYIREELKGVKIVGH
                     SMMDTMDMEAILEVNPDLIIMSQRQEKIYDQLKEIAPVVMMKDYANDWRSKLTDVSKL
                     FDKEEEAKSWLQKYDEKATKLGKEVIEKNGEKTYLPVLASSGQFMVFSDGGIGTLIND
                     DMKLARPKNMPKQDGITLPMVSMEGLTDIDADHIVVIATEADKKDLENSAIWAQIRAV
                     KDGNVTILDAAPFFSQSYNPIGKELLLESVKNELTK"
     gene            19076..19540
                     /locus_tag="ACOIAF_000545"
     CDS             19076..19540
                     /locus_tag="ACOIAF_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016208838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667572.1"
                     /translation="MVHLVYCDNAGKKGEKVLDKILAGTKTMVIRGAAGRKIPHSRVF
                     EDELLYFMEKGTAKISAKATVKKVENYVKLTEDEISKILNENQPKLNLSEKQKVRWHK
                     KCLCLVEFENVEEITPLDFDHQGNMDDWLIVEKIEDVVVGTSIPYNYEKSKF"
     gene            complement(19652..19969)
                     /locus_tag="ACOIAF_000546"
     CDS             complement(19652..19969)
                     /locus_tag="ACOIAF_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HGZ2667573.1"
                     /translation="MDEKMIEISFGIIGFAGDAKGLAFEAIKEAKSGNIDKARECIEK
                     SKESIHKAHKFQTELIQNEANGNKTEISVILIHAQDHLMTAMNFQQLAEEFIDVYERL
                     ESK"
     gene            complement(19982..20713)
                     /gene="chbG"
                     /locus_tag="ACOIAF_000547"
     CDS             complement(19982..20713)
                     /gene="chbG"
                     /locus_tag="ACOIAF_000547"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HGZ2667574.1"
                     /translation="MTKIIINADDFGYCEAVNYGIISTHNNGIVRSTSMMANMPGVEH
                     GVGLLKENKTLNCGVHMTLSCGRPLLSNLKTIVDKDGFFIRRITDEIIEKMDCDEIYR
                     ELCAQIDRVKGLGIDISHLDSHHHIHTLVSLKPVIEKIVTKYNLPIRGGFEYNLEYSK
                     VVPLIDSFYKENVSEEYFIKNIDEIMKYDVVDIMSHPAFLDDYILNSTSYAIDRTKEH
                     KILTSKKVKEFLEKNGLVISSYRDI"
     gene            complement(20739..22049)
                     /locus_tag="ACOIAF_000548"
     CDS             complement(20739..22049)
                     /locus_tag="ACOIAF_000548"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HGZ2667575.1"
                     /translation="MKKGLKIVTIGGGSSYTPELVEGFINRYEELPVKELWLVDIEAG
                     KEKLEIVGNLAKRMVKKAGVDMKINLTLDRREALRDADFVTTQLRVGLLDARIKDETI
                     PLSHGVMGQETNGAGGLFKALRTIPVIFDIIKDVEELCPNAWMVNFTNPTGIITEAVF
                     KYTNFRRYIGLCNVPIHLKNDVAKLFNVESDRISMDFAGLNHMVYGLNVSLDGEDVTK
                     EAIDKFVKADISMQNIKAIDFNAEFVKSLGAIPCPYHRYYYKTKEMLEDELREFKEGK
                     ARGQVVKELEEQLFELYKDEKLDVKPPQLEKRGGAYYSDAACNLISSIYNDKKDIQVV
                     NTLNKGSIRDFKDEQAVEVSSVITKNGPKPISIGYLPDSVHGLVSQIKSFEVLAAKAA
                     VYGDYESALLALCINPLIPSDDLAKTILDEMLEAHKDYLPRFNR"
     gene            complement(22126..23439)
                     /locus_tag="ACOIAF_000549"
     CDS             complement(22126..23439)
                     /locus_tag="ACOIAF_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HGZ2667576.1"
                     /translation="MEKFMSFMDKYIVPVAAKIGAQRHLVAVRDAFIVMIPITMVGAL
                     GTLINNLPLEAYKNLMASIFGENWTTFGGDLWWGAIGTMAVFLVIGVAYFLAKSYESD
                     GLQSGLIALSIFFIMAPQIGKIVPEGGTTVVEGWGMIQQTYLGTAALFSSILIGLLST
                     EIFVRLSKVKKLTIKMPDGVPPAVSRSFAKLIPGMLTIMIFTVIGIFIKMLSNGSFLT
                     DILNTYLGAPLSNVADSLGSTMLIAFIIHILWTVGLHGANIALPFTETILMKLGGENA
                     ALAQAGATEGYHVLAGAFFDAFVYLGGSGMVLGLIVALLIAGRRRKEMIVLGGPPAIF
                     NIGEPLIFGLPIVLNPIFMIPFVLAPVICSAVSYLAIDFGLVAPVILPKIPWVTPPIL
                     GGAMATGDWTGGALALFNLILSILIYIPFVIASEKMEANKLKINN"
     gene            complement(23461..23772)
                     /locus_tag="ACOIAF_000550"
     CDS             complement(23461..23772)
                     /locus_tag="ACOIAF_000550"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGZ2667577.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMEDAAKENGIEAKIWAISEVNLKN
                     EIENCDVLLLGPQVRYVLGKATEMAKPYNIPVEVINMMDYGRCNGKAVLDRAVELNSS
                     K"
     gene            complement(23971..24675)
                     /locus_tag="ACOIAF_000551"
     CDS             complement(23971..24675)
                     /locus_tag="ACOIAF_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGZ2667578.1"
                     /translation="MKEPIYKVIENHVRELINSDSLKEGDLIPSEKQLSEEFNVTRMT
                     VRSALNNLVKEGYITRQRGVGSIVIANNIYDNISSVSGFTKEMESKGYKVSNILVSLE
                     IVQADENLSSKLNISLEENVWEIKRVRLANDARVSYMITYMPVKLFPNLNKTHCENSL
                     YNFVEEVCGYKIAMSEREVQAVISNEECMDNLKLEEPEPLLYISQICKLQNSEIFEYS
                     HTYHYGYTLTLNAVVE"
     gene            complement(24766..26127)
                     /locus_tag="ACOIAF_000552"
     CDS             complement(24766..26127)
                     /locus_tag="ACOIAF_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HGZ2667579.1"
                     /translation="MKDRLNLTEGRITEKLLKLSLPIMGTSFIQMGYNMIDMIWVGKA
                     GSKAVAAVGTAGFFPWLAMAFIMISKVGGEIKVAQSIGANNISTTKSYMKSAIEINII
                     LAIIYTISLIVLNKQLIGFFRLGDLEVITMSRQYLIIVALGMVFYFINPVFTAIFNGL
                     GNSKTPFRINTVGLITNIILDPILIFGWGFAPKLGVAGAAIATVFAQIVVTTCFIYII
                     VKSKEEYFKLRLFKEIETKYYKVLYKLGLPIAIQSGMFTVFSMLLGVIVASWGPVAVA
                     VQKVGSQIEAISWMTAEGLSVALGSFVGQNYGAKKYSRINKGCKIAITVSSVLGIITT
                     LVLVFAGESIFSIFIDESEAIEKGAVYLKILGYSQFFMCLEIITTGAFKGLGRTYIPS
                     IIITILTGARVPLAYFISRPEMLGLNGVWWSITLSSILKGILMISLFISLLKLGKLYK
                     ISE"
     gene            26626..27780
                     /locus_tag="ACOIAF_000553"
     CDS             26626..27780
                     /locus_tag="ACOIAF_000553"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HGZ2667580.1"
                     /translation="MKINKKFNRVDMQVSILTAIIVIISSSCVYFFNYTLTYKSMIYS
                     LSERSKHIYEYVERNIDKNTFTEINSKEDQSKKSYKSSKILLESVKNTTGVMYLYTAK
                     KTKDGSLVYVVDGLNSSRSDFRNAGDLIEKEIWGDLNKALDNHILLPKKIKSTSWGYI
                     FISYFPIHNDVGKVVGVIGIEFEAKHQYNTFKFISIVTPIIALLTCLISALIAVILFK
                     RISNPTYTDFANTDYLTGLKNRNAFEIDMNNLNNSNLKNLISIISIDLDGLKKINDKF
                     GHQTGDLYIKQASKIIQYVINKKYIVYRIGGDEYIIILKNLSHKEINNIINNINLKIV
                     EENSTNELKLSMSIGYAIFDEKVDVSLFDTYRRADSQMYDNKRKINKQYQ"
     gene            complement(27862..29580)
                     /locus_tag="ACOIAF_000554"
     CDS             complement(27862..29580)
                     /locus_tag="ACOIAF_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033225 - ATPase-coupled
                     2-aminoethylphosphonate transporter activity [Evidence
                     IEA];
                     GO_process: GO:0033223 - 2-aminoethylphosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HGZ2667581.1"
                     /translation="MNNNAISSKSISLKSKMNLDIKKCMLTVLVMSLVIFLLAPIIYL
                     FVQAFLDKDGVFVGLQNFQEYLASESLLNSLKNTVFVSVISTVISLTLALVYAYALTR
                     TKIKFKGFFKSLAMFPMFAPTMMHAIGLVYLFGNQGLVTNLLGVHVPIYGAVGIILAQ
                     IIYVFPQSFQILYISLSSTDYRLYEASNTMGISKIREFISITIPSIKYSLISSAFVSF
                     TLCFTDFGAPKVIGGEFNVLSIDVYKQIIGQQNMPMGATVSILLLIPAIISFIVTKKI
                     EKNQNSYITSKSIPYKVKTNKLRDSILGGVVGIIAFGIIAVMLTVILSSLISVWPYDL
                     RLTLEHFSMKNSIDGIKPFINSIKMSLFTAILGTSFVFLFAYLNEKTEKAPYLKSFGY
                     FLSTLPMALPGLVLGIAYVLFFNKLEFNFMDTIYIKNLFNGLYGTLLIMVICNIVHFF
                     SVAFITATTAIKKVDKELDMVAKSMGINSASILKNVTIPLCLPAVLENFMYFFLNSMV
                     TISALVFIYTASSKVAAISIVQLDDKGSTAQAAALAVLILVTNILVKVIYEFIKNRLE
                     KRTNFN"
     gene            complement(29585..30574)
                     /locus_tag="ACOIAF_000555"
     CDS             complement(29585..30574)
                     /locus_tag="ACOIAF_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2667582.1"
                     /translation="MSYLKINNVFKSYDQKKVLNNISLDIEEGEFLCLLGPSGCGKTT
                     LLRIIAGLEDVNSGTIILQDKDITNLEPSKRGFGIVFQSYALFPNMTAYNNIAFPLKE
                     RKVSKEKIDNKVKEVLETVGLTNEAHKYPKALSGGQQQRIAIARALALEPKFLLLDEP
                     MSALDAKVRHKLRMDIKRLQKELNITTIMVTHDQEEAITMADKIAILNGGDIMQIGTP
                     EEIYQNPQNLFTAQFIGDTNCFDNGDSILTVRPEYVQIEKSTKENYQGIISNIEFRGN
                     LLRVEIKDKLNENFIISDVSIKEWVNLNLVEGDLVKISIDEKYYLKYPKVKGA"
     gene            complement(30670..31719)
                     /locus_tag="ACOIAF_000556"
     CDS             complement(30670..31719)
                     /locus_tag="ACOIAF_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033225 - ATPase-coupled
                     2-aminoethylphosphonate transporter activity [Evidence
                     IEA];
                     GO_function: GO:0033226 - 2-aminoethylphosphonate binding
                     [Evidence IEA];
                     GO_process: GO:0033223 - 2-aminoethylphosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HGZ2667583.1"
                     /translation="MKEFIFTCLCIVVITFMTSCISTTPQTTQGKGNNKLTIYAALEE
                     EQIAYYMEGFREEYPNIDVEIVMDSHGVIASKLLAEKDNPQADIIWGLSAMNMIELKK
                     DGALEAYKPKNYDKVDKKFKDTEDDVSWVGMSVPETAIVVNYKELKKLGIDIPKSYED
                     LIKPEYKGLITMPNPASSGTGYLTVSGLIQMMGEKDAYNYMDKLHKNIATYTHSGSKP
                     AKLAGSGEYPIGITLGYRATQQLSSGEPIEVVFPKEGSGWDLEANALVKKDNIKKEAK
                     LFLDWAISDSAMDRYAHEVAVTSIKTDNPVPKGYSKQPFEQLIKEVDLNSAAKNRSTV
                     LKTWESKYGSKSESK"
     gene            complement(31996..32664)
                     /locus_tag="ACOIAF_000557"
     CDS             complement(31996..32664)
                     /locus_tag="ACOIAF_000557"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HGZ2667584.1"
                     /translation="MARLNINPTRMEMTRLKKLLKTATRGHKLLKDKLDELMKQFLEI
                     VRENKRLREEAENALDTAYKNFIIARAVMSQEYLGSALMMPKQSVSVDVSTRNIMSVD
                     VPVFDFKTENNQSDIYPYGLAFTSGELDSAMEAFSDAMQPLLRLAESEKSAQLLAQEI
                     EKTRRRVNALENVMIPNYIETIKYIAMKLEENERASTTRLMKVKDMVLKKALEEKKKN
                     DLVV"
     gene            complement(32670..34043)
                     /locus_tag="ACOIAF_000558"
     CDS             complement(32670..34043)
                     /locus_tag="ACOIAF_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HGZ2667585.1"
                     /translation="MIKEYKSIQEVAGPLMLINGVEGVKFDELGEIELSNGEIRRCKV
                     LEVNGDTALVQLFESSTGINLAESKVRFLGKSLDFGVSPDILGRVFSGMGRPIDGGPE
                     IIPDKRLDINGAPINPAARDYPAEFIQTGVSAIDGLNTLVRGQKLPIFSGSGLPHANL
                     AVQIARQAKVRGTDSKFAVVFAAVGITFEDAEFFISDFKATGAIDRSVVFVNLANDPA
                     VERVSTPRMALTAAEYLAFEQDMHVLVIITDITNYAEALREVSAAKKEVPGRRGYPGY
                     LYTDLATMYERAGKMKGHEGSITMIPILTMPEDDKTHPIPDLTGYITEGQIILSRELY
                     KKDIQPPIDVLPSLSRLKDKGIGKGKTREDHADTMNQLFAAYSRGKDAKELMAILGES
                     ALSEIDLMYAKFADEFEKEYVSQGFRTDRTIEQTLEIGWKLLSMLPKSELKRIRDEYF
                     EKYMPKE"
     gene            complement(34040..35818)
                     /locus_tag="ACOIAF_000559"
     CDS             complement(34040..35818)
                     /locus_tag="ACOIAF_000559"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HGZ2667586.1"
                     /translation="MKTGTIVKVSGPLVVAEGMRDANMFDVVRVSDKHLIGEIIEMHG
                     DKASIQVYEETSGLGPGEEVVSLGMPMSVELGPGLISTIYDGIQRPLEKMYDISGTNI
                     TKGVEVSSLDRTTKWEFKPKKSVGDKVVAGDIIGGVQETAVVECKIMVPYGVKGTIKE
                     IYSGEFTVEDTVCVITDEKGNDIPVTMMQKWPVRKERPYREKKTPDSLLVTGQRVIDT
                     FFPITKGGVAAIPGPFGSGKTVTQHQLAKWADADIVVYIGCGERGNEMTDVLLEFPEL
                     KDPRTGESLMQRTVLIANTSDMPVAAREASIYTGITIAEYFRDMGYSVALMADSTSRW
                     AEALREMSGRLEEMPGEEGYPAYLGSRLAQFYERAGKVNCLGMDEREGTLTAIGAVSP
                     PGGDTSEPVSQATLRIVKVFWGLDASLAYKRHFPAINWLTSYSLYQEKVDVWADKNVN
                     DNFSAQRAQAMKLLQVESELQEIVQLVGVDSLSDGDRLILEVTRSIREDFLHQNSFHE
                     VDTYTSLHKQYRMMGLILKFYKSAQDAIKQNVTINDIIKLSVLEKIGRAKYVEEGDID
                     AAYDSIEDEIDNELADLIKKRGAEEL"
     gene            complement(35935..36252)
                     /locus_tag="ACOIAF_000560"
     CDS             complement(35935..36252)
                     /locus_tag="ACOIAF_000560"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HGZ2667587.1"
                     /translation="MYKVGVVGDKDSIMGFLALGIDIFPAYDSDEIKKSIHKLVEDEY
                     AIIYITEQASLLAKESIAKYKDYQLPAIIVIPGIGGSMGLGMNEVRESSKRAIGADIL
                     FKE"
     gene            complement(36245..37222)
                     /locus_tag="ACOIAF_000561"
     CDS             complement(36245..37222)
                     /locus_tag="ACOIAF_000561"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit C"
                     /protein_id="HGZ2667588.1"
                     /translation="MAEEKTYPYAVARIRVLEKQLLNRQMFIQMAEAKSPEDSLRIIA
                     EAGYADTSNNNIHNFENILTQELSKTYEILKSLAPEEKFVDVFLYKNDYHNLKVLIKE
                     EISGVDGEKYLIDGGTIPLIKLRESLANRSFSDLPKIMSSAILEAFDVYNKTQNGQMV
                     DIVLDKATFTSMKETAKESQNKFVIDYVMKVCDLTNLKSFIRVKNMKKDFDMFMNVFV
                     SGGSLDKEKFLEAFSSDTPASCFKSTSYSDVCKNGMDSGFTVFEKLCDDYLMEYVRGA
                     KFKPLTLEPLIAYLYAKESEIKTIRIILTSKLNNIDADTIKERLRDAYV"
     gene            complement(37237..37800)
                     /locus_tag="ACOIAF_000562"
     CDS             complement(37237..37800)
                     /locus_tag="ACOIAF_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HGZ2667589.1"
                     /translation="MGNEQKMIDRIIADAKQEAQEILDKAKSEADLKVNSANEKAEKE
                     MASYTKLAEAEAEKAASKEISGAYMEAKKQILSKKQEILEEVILEAKNKLLNLKDNEY
                     EEIILNMIEKSNCTDDSEIVLSKKDKKTLKDVLSKKGIKVSDETRDITGGFIVKKGDI
                     EYNYSFEAIIAVEHEYIEQIAAEILFN"
     gene            complement(37852..38343)
                     /locus_tag="ACOIAF_000563"
     CDS             complement(37852..38343)
                     /locus_tag="ACOIAF_000563"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HGZ2667590.1"
                     /translation="MEFLNALGNGQFLAISGAAIAALFAGMGSAKGTSIAGQAAAGVV
                     TEDPSKFGQLLVLQLLPGTQGLYGFIIAFLILSKAGIIGGDAIPTSAQGLQLLMAGLP
                     IGLVGLVSGIAQGKAAAAGVGIVAKRPEELGKAITFAAIVETYALLGLLVSFLAYNGI
                     AIG"
     gene            complement(38346..40271)
                     /locus_tag="ACOIAF_000564"
     CDS             complement(38346..40271)
                     /locus_tag="ACOIAF_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HGZ2667591.1"
                     /translation="MAIVNMNKISIVGLEAQKSQILKLLMNRGFVQIDDSAFLTEEEE
                     LKNYLVKDSEESEVIMLEQKMSHVNQAINIVSSLVKQKKSLLAPKREFNKIDSEKEGQ
                     VYAEALELNNLHKEIGTIRSNINLLNNNKSMLEPWVNFDIPIENMETKYTKTILGTVP
                     ISANIDALRVKLEDEIPESVIGIVNSDKLMNYVYLITLKETFDDVLEVLKEFNFSVVS
                     LPNEEGTPLQAIKKYNEMIIRQENVSKDKTEEIKRHADSIHMLENLYDFYTIERDQKK
                     IIERLVKTKTTFCLNGWLPSKRADELIKEITEKFDCCVQTEEGSKEEGFPILLENNKL
                     VTPFESVTNMYSYPSTKDIDPNTILTFFFVIFFGMMLSDAAYGLIIAVVCGFVVYKLK
                     IQKGEGNLIKLIGICGVSTTVWGLIFGGILGDLIPIKALINPLEDVMTLMGMSLLFGI
                     IHIYVGLGIKAYTLIRSGKVIDAIFDIGFWYLCITGVCLLIIPFVAGDIGVFSEVGKY
                     LAIIGAIGLVLTQGRSFKGIPVKLFKGFSSLYGITSYFADILSYTRIMALCLTTGVIA
                     QVINLLGAIAGPILAVVIGVVGHTINLLINALGAYVHTSRLQYVEFFNKFYEGGGVPF
                     VPFKYKTKYTSINKKEM"
     gene            complement(40273..40584)
                     /locus_tag="ACOIAF_000565"
     CDS             complement(40273..40584)
                     /locus_tag="ACOIAF_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667592.1"
                     /translation="MAFEIIKSIVEAEQTADSIKVKAVTDAESIRADAVNKCESIFAD
                     VKKQAKLMEETLIEKAVTDSRAEVDKILANAKSECLKIEKTAEERKSKAIEAVIGKVV
                     R"
     gene            complement(41214..41489)
                     /locus_tag="ACOIAF_000566"
     CDS             complement(41214..41489)
                     /locus_tag="ACOIAF_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif11-like leader peptide family natural product
                     precursor"
                     /protein_id="HGZ2667593.1"
                     /translation="MNEKLTKILEEAKNDDEFRKELIKTREQRDVMDSFCKVVTSRGY
                     DMTVGELFSMGEEYTSNLLKSVNGGAAYPIEDWSDWYEDFFIMLSNI"
     gene            complement(41604..43013)
                     /locus_tag="ACOIAF_000567"
     CDS             complement(41604..43013)
                     /locus_tag="ACOIAF_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HGZ2667594.1"
                     /translation="MIDGKEVKQESGQKNLKFKIAIVVGVLFVAYIGMAIFFRNHFYF
                     GTTINGVKVSGKTVEQVNDLLTSSTNSYTLNLEERNNKKEQIKAKDIDLKFTPDDKVQ
                     KIKDSQSAIGWIGGIFKSKEYNNMVNLSYNKDLLEKRFNALSCFDSKNVVEPKSAYPK
                     YDSTKNAYVIVPEVLGNKVKKDDFYKLVQNSINSGNTSISLDESKVYEAPNNTKDSEK
                     LKQAIKTLDKYVGVVVTYDFSDRKETLDGSEINKWLKVDSEKNYDITFDNDAMVEYTR
                     GLSKKYSTFGDSRPFKTATGKDITISGGIYGWLIDKKKEAEALVDVVKAGKNVTREPI
                     YIQKAVSRNKDDIGNTYVEISLSGQHMWFFKNGEMLVSTDVVTGDLARGFATPSGIYP
                     LNYKARNVSLTGQGYSSPVSYWLPFNGNIGIHDATWRNSFGGSIYKSSGSHGCVNTPF
                     SKAKKIYENIEPGTPIILY"
     gene            complement(43384..44046)
                     /locus_tag="ACOIAF_000568"
     CDS             complement(43384..44046)
                     /locus_tag="ACOIAF_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HGZ2667595.1"
                     /translation="MIDVSIDDKLKERWPNAKLGCIQAKVAVNKSSEKLINEINEFCD
                     TINQSLKVEDITKLDKIKDARNAYKELGKNPSRYRTSSEALVRRIIQGKGLYTINNIV
                     EINNLISIKSLYPVGTYNVSKLHSPVCFTVGDEGEQYKGIGKELINIENLPILSDSVG
                     KFGSPTSDSERAMITEDANEILICIFSFSQESDIEEYFGYAKELLKEYADGKDIETKI
                     IK"
     gene            complement(44300..46519)
                     /locus_tag="ACOIAF_000569"
     CDS             complement(44300..46519)
                     /locus_tag="ACOIAF_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HGZ2667596.1"
                     /translation="MTGRNKSFTLITILFLTSLFLTMGYIYIEDTKHLLMSEAKIHIK
                     EVAVQGSQLAQRQIEKDLDVLNIFSNYYASNPDIPNEEKMKNLLDEMENQKFYTMAIV
                     DINGNAENTKGDKFSVKDREFFKNSIKGKKYVSSPYVDEVNKSIKKIAISVPLLNNDK
                     VVGVLYCTYNINTLMKLINISFYENNSISYVVKNDGTIILHPQGDSLSKNIYKLLEQD
                     NDIQEVNRLKKELQENKTGATVLNMLEERRYLGYATMDNGNSENNYIKDWNLIFSIPE
                     TVVFSNSKQIINRAVYAVLSIVLIFIAIIFYIIYIKKSNEKEILSLAYEDKVTYIGNQ
                     NKFYRECSKYLLDKPSLNYIIVYFDINNFKMINDTFGYEFGDNLLITIAKALKEELTE
                     GEVYARLSSDYFAIFCDYKNGRNKIIRKLDNIRSNIESNLSIVFEISLCVGIYFVEEG
                     EVDIQKAVNKANMARSVAKGKNINYAIYNEDVRNKLSEESMILDDIKIALVKNQFEVY
                     YQPKFSLVTGEMIGSEALIRWNHPEHGFISPAVFIPIAEKSKLILKIGRFVFERVCND
                     LSEWKKQGKKIVPVSVNLSRVELYQPDIVKFINKTIQMYNLSSDFIEIEITETVAINE
                     LNILKNVLNELRKHGFSISMDDFGTGYSSISCLRDMPIDILKLDKSFLGGIEHDERSR
                     NIAKSIVSLAKSLDLVVIIEGVESKEQAELMKQFGCDLVQGFYFARPMPAKNFLDLL"
     gene            complement(46795..49449)
                     /gene="adhE"
                     /locus_tag="ACOIAF_000570"
                     /gene_synonym="adhC"
     CDS             complement(46795..49449)
                     /gene="adhE"
                     /locus_tag="ACOIAF_000570"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HGZ2667597.1"
                     /translation="MAHKEQVIIENKKENEVMSVGLDTVNNVESLVKKLAKIREAQKI
                     FATYSQEQVDKIFLAASLAANKQRVPLAIMAQKETGMGIAEDKVIKNHYASEYIYNAY
                     KETKTCGVIEKDEAFGMTKIAEPIGVIAAVVPTTNPTSTAIFKALLALKTRNGIIFSP
                     HPRAKNSTIEAAKVVLEAAVLAGAPEGIIGWIDEPSLELTTTVMKEVDLTLATGGPGM
                     VKSAYSSGKPAIGVGAGNTPAIIDDSADIKTAVNSILVSKTFDNGMICASEQSVIVLE
                     NIYNEVKKEFKERGAYLLDKDETEKIRNIILVNGGLNSKIVGQTACTIAKLAGFEVPV
                     DTKVLIGEVESVEIEEAFAHEKLSPVLAMYKASDFDDAVRKAEKLVEDGGFGHTSSLY
                     IDDVNQREKLDKFTSAMKTCRILINTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVS
                     ENVGVKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVALKELKDVMNKKRAFIVTD
                     TFLYNNGYTKAVTDLLDEMNIKHTTFFEVEPDPTLECAKIGAKAMREFNPDVIISIGG
                     GSAMDAGKIMWTLYEHPDVDFQDLAMRFMDIRKRVYTFPKMGEKANFVAIPTSAGTGS
                     EVTPFAVITDQDTGVKYPLADYELMPNMAIVDSDMMMNMPKSLTSASGIDALTHALEA
                     YVSMLATDYTNGLALQAIKSIFEYLPRAYDNGAKDPEAREKMANASTMAGMAFANAFL
                     GVCHSMAHKLGAFHHVPHGVANALLITEVMKFNSSDAPKKMGAFSQYKYPEALKRYAE
                     IASFLGLKGNSDEEKFQSLLVAIEDLKLKVGIPKSIKEFGVEESKFMDSIDEMVIQAF
                     DDQCTGANPRYPLMNEIKDMYLNSYYGR"
     gene            49953..50153
                     /locus_tag="ACOIAF_000571"
     CDS             49953..50153
                     /locus_tag="ACOIAF_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667598.1"
                     /translation="MKKLKTISIFSLIISVILTIGGIGIVTYYVDNLFIRGLSVFVLI
                     MSSSFVSTTVRLIFEESKRYKF"
     gene            complement(50360..50950)
                     /locus_tag="ACOIAF_000572"
     CDS             complement(50360..50950)
                     /locus_tag="ACOIAF_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit B"
                     /protein_id="HGZ2667599.1"
                     /translation="MRLEGLTIGVGFTGSFCTYDKIFIELENLVKEGANVHTIFSDVS
                     QNIDCRFGNSEEFMKKAYELTGNKPIVTIEEAEPFGPKGIADIIIIAPCTGNTAAKLA
                     NGITDSPVLMAAKGHLRNDKPLVISISTNDALSFNFKNIGILLNSKNIYFVPFGQDNC
                     KAKPNSMIAHTELIIPTIELALENKQLQPVIKSPHQ"
     gene            complement(50953..51828)
                     /gene="dpsA"
                     /locus_tag="ACOIAF_000573"
     CDS             complement(50953..51828)
                     /gene="dpsA"
                     /locus_tag="ACOIAF_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit DpsA"
                     /protein_id="HGZ2667600.1"
                     /translation="MSNQYDITVIGGDLRQVYMVKKLINKGYSVVTYGISNEIINGIV
                     GKSTSLAEALSKSSIILCPIPFTKNQVNIENTDDCLDATIDNLLINLSPNQILFGGNI
                     PTKVCKFCEQHSIQIYDFMKMNDVAILNAVATAEGAIAEAIKRSIINIHQSNCLVLGY
                     GRCAQILAKKLYALDANVCVGARNNDARSTANAFGYSSISLSQLDEKLLQFDYIFNTI
                     PECILTKERLEKVSQEVTIIDIASSPGGVDYSVVKEAGINATLCLGLPGKYSPKTSGE
                     ILVNAIENIINERRD"
     gene            complement(51904..53295)
                     /locus_tag="ACOIAF_000574"
     CDS             complement(51904..53295)
                     /locus_tag="ACOIAF_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HGZ2667601.1"
                     /translation="MNYYELLRIKKERHKNKNFLIIDGEKYTYENILSYSEELGKQIS
                     LGENIPILIYSKNIMFQLISFFAINYSKNIPIICHYNLSKKVLDDILLKNNISIIISD
                     ESMDVIDLYDDNNNEVAKKVNFSIFNPKFNIYMYQYKNLINYLDKSICMGALSSGSTS
                     VPKVLYRTYKSWAGFFPIQNDIFNISGESILFVNGTLSFTGNLNSIMSVLYEGGSIVI
                     SSGLNCKSWIRTIKQYNVTNIYLIPTKLQLLVRHLKEPVLKVVSIFTGSQLLFEDTAR
                     NLKKYMPKSEIILYYGASELNYITYLCYDELIEKPLSVGRPFPGIDIYIREGKIFVNT
                     EYAVYNVTKPYSVNDIGYYDNDGYLIFEGRSDDIINIGGFKVSSTKIENEVKKIPQIE
                     DAIVLPYSDTIRGNQIVLFVITIDKITKKDLLIKMKDNLMKNEIPKKIIFLNSFPYTS
                     SEKIDRLALLKML"
     gene            complement(53299..54435)
                     /locus_tag="ACOIAF_000575"
     CDS             complement(53299..54435)
                     /locus_tag="ACOIAF_000575"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HGZ2667602.1"
                     /translation="MKKVFILGGLRSHIGLKNGIFQFVQPELLGASVLKDLIKKYEID
                     KIDEIICGNAVGTGGNIARLMTLTAGVSNEVPAFTVDMQCASAMMSIDIAFSKVKSGQ
                     CDLIIAGGFESSSLQPMRTYHKNDKRYNTNNPNYTVAQFSPDDNSQNSMLEGAERVAE
                     LYQIEKADLDFWVKESHKRAKEAREEKILEDIISPINNSTCDEGIRDKMSQRLLDRMP
                     SILGKETITNAANSCLINDGASFIIICSKKYLEHVKKKPKAKIINTCTIGTDATLSPT
                     SAIQAMDKLLEIESINYLDVSAVEFNEAFAVIDVLFQRKYPELIDRYNIFGGALAYGH
                     PYGASGAIIALHLLKALEKTKGRYGICSIAAAGGLGSALLLERV"
     gene            complement(54467..55012)
                     /locus_tag="ACOIAF_000576"
     CDS             complement(54467..55012)
                     /locus_tag="ACOIAF_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HGZ2667603.1"
                     /translation="MKNTKVNIQDLTKMSICVALLCISSYIYIPLPFTPAGVTAQTIM
                     INLIALILTPRQAFSTVGVYIMIGLIGIPVFGGGTSGIGKLLSPTGGFYFGFLFAALI
                     ISLLKGRNNDIKRYILITIFVGMPIIYFMGTVFMCYFNQMTVEAALFAAVVPFLIGDT
                     IKSVIASFLGTKLNNVLRRNL"
     gene            55336..56115
                     /locus_tag="ACOIAF_000577"
     CDS             55336..56115
                     /locus_tag="ACOIAF_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667604.1"
                     /translation="MKVNENNINNIDEELDELSRKVYLESPDFWISISDKIIDKVFDE
                     MLLFFAVKYNYFNIVCFAIENNYLDLNMPSKNKKYTNIMEHLLDTSKQNEDKKIYNYL
                     LSLNKGNNLDERPILDELNCNISSDTFEKKDIYLPKYLCPSCNSNIFDTGYKVSNDIT
                     YKFSSKEAEPIEMCSEVSSIRCCNCDELLEDITLDKLYNLCKIQNCNNCGSNLTKVGI
                     IAKKKMNFDENTEKFKDVSTSYCCGNCDSEINLSQREHFKI"
BASE COUNT        18970 a         9596 c         6896 g        20825 t
ORIGIN      
        1 aatttctaaa acggttagtt tgacattatt ggaatataaa caaaaattta atataaattc
       61 gttaatatat taatacattt ttaagaatga caaggaataa attataacaa attttaggaa
      121 tatataataa tagggtctca gaacttagtt acaagttcta aaaccctatt tgtttagtgt
      181 aaaaaacatc ataaaattta gcttaaatct tttcacataa tattactctt tttctaagtg
      241 ataataaaat atatctattc ctctaaaaac tgtaaaaatt cctgattaaa tagtttaagc
      301 tcatcataga atatagcatg accactatac tcaaaagtat ataaaattga atcttctatt
      361 ttttcattca taaaaactgc aaattcatac ggacatattt catctaattt cccatgaaat
      421 ataccagtag gcactcttac acattttaaa tcattaaata attcttcatc tcttagagat
      481 acagcagttc caatagtgcc aatacctgat gcactaaaac caatatcatt aaaccatctt
      541 ctaaagcttt ctggtggatt tgatgcaaat actttttcgc caaaatcggt taccatttct
      601 ggtctatcag tacatgcttg tgctatcaat ttatttacat cttcacgtgt cataccatat
      661 ggaaattctg gaggtctttg tacaaatgat ggtgcagcag ctgcagctaa taccaatttt
      721 gacactccat atccattaaa taaactcata tacctaagta cgattgctcc tcccattgaa
      781 aaaccaacaa gggtaaagtc ttttaatcca atagcatgta ccaccttata aatatcgtct
      841 gctaattggc tataagtata tcctcctgat gttgcatcag atttaccaaa acctcttaaa
      901 tcaattgaaa ctgttctata tccgagtttt ggcaatatat ttgtttgata ttcaaatatc
      961 ttatgtccta agggccaacc atgtataaaa agtacagttt ttttagcact cggattcaaa
     1021 tcatatactg ctatgtttat atcattcaca tatacataat acatatcaac agcaactccc
     1081 ttcaagaaaa tgtatgatgg ttattttata tatatgaata aaatgatgaa taactaaatt
     1141 ttcaaattta tttacaatac ttctctattg ccttttcaaa taaacttaca tgaagttttt
     1201 catctaggat tattctattt aatatagctt ttatataagg gtcttcaata agacttatat
     1261 ctttataata attattaata gccacatgct ccaaatgcaa atctagtttt aatctgttac
     1321 aaatagaatt tccataacat acaaaaccac cattccaata tgcaccacaa gaacttgtag
     1381 aacctctata tacaggattt ccacccaata atcttatagt ctttgctaat atctccatat
     1441 gtaacatttc tgttatagat atatttatcc acatttcact taactctcta tgcgtttcat
     1501 ctaaatcaaa attatggtat agatattgat ttacactcgt aaattcactt gatactccgg
     1561 catagtcatc catcaataat tctgcatagt acttatttgg acctaaaact tttatctctg
     1621 gatatggctc atcacttgaa tatccatttc gcttgtgcat gtcataatgt gcatcactca
     1681 taatatctcc tcctttaaat ttaatgtatg atattaattg tatgcatcac aactttttca
     1741 tataacttag ataataaaat ctacttaaat aataaaaagc catattaatt aaaattaaca
     1801 tgacctttta tccacaaaat tactttttaa ttattcatct tttccagcta ctgatagctc
     1861 tttaataaca actgaaggcg aacctatact actcattgga aattctaaat ctgctcctat
     1921 agcttcaata tcttttaaca ggtcaaaata gtttcctgct actgtaattt gctctactgg
     1981 aaatactttt tttccttctt tgatataaaa gcctttggca gctaaagaaa agtctcctgt
     2041 tactgaattt gcacctgaat gaagtcctgc aaagctagta accatcaatc cttcatctat
     2101 atcctttata atttcatcta aagatttatc tcctttctct atataaaaat tagttggata
     2161 tatgcttata ggtgatgaat atgaagattt tattccattt cctgtagtct ttacacctgc
     2221 cttattagct gtttttaagt tatgaagtaa agttataagc tttccattta aaacaacttc
     2281 ttttttaaat gtagctactc cttcatcatc aaaaggagta gatgccatac cattttctaa
     2341 aagtgggtca tcaactattg taactatagg agatgctatc atgtctcctt ctctattttt
     2401 caataaagat aatccttttt gagctgaatc tgcattaaat atcccagaaa atgtgctaag
     2461 caaagatacc atagcttcat taagcaatat agttttatat ttacctgatg gaatactctt
     2521 accacctact ttagataaag cattatcaac accaagtttt gctatctcac aaggttttat
     2581 ttcttcaata gaatttgcta tattatatcc tattccatca tatttttgtc cattgtcttc
     2641 tatgatagga actacaaacc ctataagcat attagtttta ttgcttaaat taagaccttt
     2701 agtattagat ataccattac ttgatacaga ataagatata gtacatttag ataaatttac
     2761 caccttatct gaataagctt tagtctctct ttccatttct aaagctaaat caatcaactt
     2821 atctgcctct aaattttcta gctctttaga ataagtcttt acatcattat agtgcttatc
     2881 tccttcatat ataaattgta catcttcatt ttcaatagtg tttactccat cttttacatt
     2941 ttttatcaac atatcaacag ctttatcatc taaaatttct gtataagaat aacctatctt
     3001 tccatttacc ttacctctaa aagataaacc aaaagattta tcaagattgt atttctcaac
     3061 ttctccttca tatatattta tactcaaatt ttctcctgta taataatata tttcacactc
     3121 ttcaaaacct tcacttagag cctttttaaa taatatatct ttaaaatttt taagttccac
     3181 aattatccct cctatcttcc acctacagta atttctttta cccttatcat aggttggcct
     3241 acattagttg gtatacttcc tgatgatgag ccacacatcc cctgagcttg ttttacgtta
     3301 ttcccaacca tatctatatt cataagtata tcgctacctt ttccaattaa actagcacct
     3361 ctaactggct cttgtatttc tccatttttt attaagtaac cttctgaaac tgcaaaatta
     3421 aactctcctg ttacaggatt tacagaacca ccacccattt ttttagcata aagtccatct
     3481 ggtattgact ttataatatc ttctggtcta tcctctccag cagcaatata agtgttagtc
     3541 atccttgaag ttggtgcaaa tttgtagttc tgtctacgag aacttcctgt aggattcatt
     3601 cccattcgtc taccattaag tttatctatc atataagact tcaaaattcc attttcaatc
     3661 agtatattct tttgagttgg atttccctca tcatctatat tcatagaacc ccagtagttt
     3721 ggtattgtac catcatctat agctgtaatc tttgtggaag caatctgttg acctaattta
     3781 tctgcaaata cagagttccc ctttgcaact gatgtagctt ccaaagaatg tccacaagct
     3841 tcatgaaata ttactccacc aaatccatta tctattgcta ccatcatatt acctgcagga
     3901 caatttttag catgcaacat agtatgagct actctagctg attcttttgc ataatattct
     3961 gggtctattt cttcaaatat ttcaaaccct ttacaacctc caggaccttc aaatccagtt
     4021 tgattttcat tacccttaga tgcaattgaa cttatcccta gtctggttct tattcttcta
     4081 tcctcaacat atataccttc agtgtttgca attaatattt tttggtcttt atccaaatag
     4141 ctaacactta cttgagatat atcattacta aattcttttg cactcttata tgcaaccttc
     4201 ataacactta ctttatctct atttcctatt gaatttggat aaatttttat attatgtatg
     4261 ttatttattt taatactgtc atttaatatt acactttttc catcttccaa tttccctaaa
     4321 gcaacagcag ctttgtatgc tacatccaac agactattta atgaattgtc acttgtataa
     4381 gcatatatac ttttcaatcc tttaaatatt cttattccta tgccataatt tcttccacct
     4441 attgcatttt ctactttatt atctattaaa ctaatagaat tatttatagt gtcttcttca
     4501 aaaatctcag caaaatctcc tccagtaatt agacactttt ctaatacctt agaagctata
     4561 ttttttgaaa gcataaattt acctcctact ttaaattttt aattaacttt tattcaatct
     4621 ttttacttgt taacaaacat tatatactat attacttaaa ataaggtaat tatgaatata
     4681 ttatacatat gttttatact aaaatgttat cacattttat ctatacatac ccatataact
     4741 agataattaa aatgtataat attatttgct atagaaaata atacactcat acataataaa
     4801 ttttaggagt gaagtatatg aatcaagaat atgtaaaagg aaatcctatt tttactaata
     4861 ttaataaagt tccaagacaa tttccatatc ttactgatga tattgatact gatgttataa
     4921 ttgttggtgg tggcgtaact ggatgtatct gtgcttacta tctagctaaa aacaatataa
     4981 agtctgtcat tcttgaaaaa ggaaggattg cacatggaag cactagcgtg acaacttctc
     5041 tattacagta tgaattagat gacaatttga tagatttaac agaggttatg actctaaaag
     5101 atgctctaaa agcatataac ctttgtactt cagcacttga agaattagat acatttatag
     5161 agctatatgg aaataagtgt gattatgcca aaagagatac tctactttat actgccaaca
     5221 aacttgaagt taaggctatc aaagaagaat ataacttaag aaaagaaaat ggatttgatg
     5281 ttgaatatat tgatgaatcc acaaaccctt ttagctttga tttaaaatca ggattgatag
     5341 caaagaatgg aggacgagaa ttagacccat ataaatatag tcaccatttg attgatgtaa
     5401 gtttaaaaaa tggtcttcaa gtatatgaaa atacagaagt aaaaaaagta gatttatcaa
     5461 atgataaggt tactgctgaa gtatcgtatg gatacaaagt tcatggtaaa aaattaattg
     5521 tagctacagg gtacaataca agtttattta caaaaagaaa ttttgcaact aagtcaaata
     5581 cttttaatat agcaacgaaa ccacttaaaa acatagcttc ttggaaaaac aacattttaa
     5641 ttagagataa ctgtgaccca tataattatc taagaactac taaagacaat cgattaatta
     5701 taggtggtga agatgtaagt tttgatgata tagaaaatga aactttagcc aatgaaaagt
     5761 acgatatttt agaacaaaga ttgaaaagta tgtttaagga tataaaagac attgaagttg
     5821 aatataaata ttgtggttgt tttgcttcaa ctttagataa tttaggtttt attggtccag
     5881 ataataaaaa taacaatcta tggtattgct taggttatgg agcaaatggt atactctttg
     5941 caatattagg aggtataatg ttaagtgaat tatatcttgg caaacaaaat aaaaatatga
     6001 aattatttaa agtagataga tttgataaat agatattata tattaaaaga ctgtaaaaag
     6061 atgaaacttt ataattctaa aagttaactt tgatacagtc ttttataatg ttccttttaa
     6121 aaatacttta tttataatta aatataaagt aaaatgttaa aatataactg ataaaattta
     6181 gtcataagat aatatttaag tttatttgat gaaatatgga ggaattatta tgaaggaaat
     6241 actcgttatt gctcccacta aaggaaccta tgaaaaatca attcacatag ttaaaaaaaa
     6301 caaatataca aatattgatg ttgtatttgg caatctaaaa gaaggtatac ctcttgctga
     6361 aaaaagtatt aatcatggta ctagaattat cataagccga ggtggaactt acaatatgtt
     6421 aaaagctact tacaatatac ctatagttga aattaaagta gatgcttatg atattataaa
     6481 aagttacaaa gaggtaaaaa attccaacga accatttgga ataattggat ttaataatgt
     6541 catctacggt ttcgatatta tagaagaaat attgaataag aaaataacta tgattgaaat
     6601 tgaaaaagaa gaagaaattt atgatgctat agaaaagtac agaaagaaag gtataaacac
     6661 atatatagga gataccactg ttgcacatat tgttaagaga cttaattgta agggaatatt
     6721 aatcgaatct agagaagaaa acatacttag agccattcaa caagcagaac aaatactcga
     6781 agcaaccaag gatgaacaaa aaagaagatt acagatagaa gctatgacag attttgtaca
     6841 tgatggaata ataacagttg ataaaaattt taaaataaca ttatttaata agagtgctga
     6901 aaaaatattt ggtataaaaa aagaaaatgc actttatcat aatgttgtag atgttatccc
     6961 aaacacagtt cttccacaag tagttgagac tatggaacct caaattggtg aaatacaaaa
     7021 tatagggagt acaaaaatta ttacaaatag agttccaatc attgttgatg gtcagattca
     7081 aggagctgtt tcaacatttc aagatagcaa ggagataagt tcttttgaac atacaataag
     7141 aaggagctta ttaagcaaag gtctatctgc taaatacact tttaatgata taattcatgc
     7201 tagtgaaaag acaaaagatt gtatagaact tgcaaaaaaa tatgcttgtt atgatactcc
     7261 tatgattatt actggagaat ctggagttgg taaagaacta ttttgtcaaa gtatacataa
     7321 ctatagtaag cgtaaaaatg caccttttgt tgctgtaaat tgtgctgcca tacctccatc
     7381 tctaatagag agtgagtttt ttggttatga ggaaggttca tttacaggtg ctagaaaaaa
     7441 aggtaaagca ggtgtattcg aactagcaca tgaaggcaca atcttcctag atgaaataag
     7501 tgaaatccct cttgaacttc aaggtcgatt attgagggta ttgcaggaaa aacaagttat
     7561 gagaattggt ggtgataaag ttattcctat agatgtaaaa attatctgtg cttcaaataa
     7621 agacttaaaa aatatgatgc gcaatggttt atttagacaa gacctttatt ttagaataaa
     7681 tatactaaca ttatatcttc cttcattaaa agaaagaaaa gaagatatta taaaattatc
     7741 agaatttttt atacataaat attcaagcaa atacaataaa acgcctttaa ttatcactcc
     7801 aaatattaaa aaggcacttc ttgaacgaga atatgaaggt aatataaggg aattggaagg
     7861 tatgatggaa agggctgtta ttgtggaatc atttgactcg attttattag aaaagcttag
     7921 caatgaatat acaaataata tagttgataa agataatagt gattttgaat gcagtaattt
     7981 agatttaaaa actttagaaa aaaaatatat ctataaaatt tataacgaaa ataatagaaa
     8041 tactacaaaa acctgtgaaa ttctaaagat aactcgctcc actctatggc gtaagttaaa
     8101 agaaatagaa gatgatgttt aaaaataata catatctata tatatcaaaa cgttttatgt
     8161 ttaattttaa tacataagac gtttatttgt tttcaataaa aaatacaatt atatgataaa
     8221 aagaatcttt gtaagtgaaa ttctcgtcaa tcatactttt aattataagt aatctacagc
     8281 atttcaatcg attattaaaa ataatcataa taactaaaga tacaaactct atatattggc
     8341 atagtaattg cttttataat acctatataa aaatgaaagg gagtgatact agatgcacat
     8401 cttaaaatca gtgaaaaaga taccaggagg acttatggtc tgtcccttgt tatttggagc
     8461 actatttaat acagtatgtc cacaagctct tgaaattgga ggattcacaa catccttatt
     8521 taaaactggt gcaatgagta tacttgctct attttgtcta tgcaatggag ctcaaatcaa
     8581 tatcagacaa gctggtaaac cattgactaa aggtatagta cttacagcaa caaaatttat
     8641 aattggtgca gtattaggta ttattgttag taaatttatt ggacctaaag gtataattgg
     8701 tattacacct cttgccatag ttgctaccat gactaattca aatggaggtc tatttgctgc
     8761 tcttgcaggt gaatatggag actccacaga tgttggagca atatctatct tatctctaaa
     8821 tgatggtcct ttttttacaa tgcttgcttt tggagtaact ggacttgcaa atgtacctat
     8881 agtagcacta tttgcagcac ttataccaat actacttgga tttatacttg gtaaccttga
     8941 tgaagatata cgagaatttc ttgcacctgg tacaacatta ttaattcctt tcttcgcttt
     9001 tcctttagga gcagcattaa actttaatca aattataact gctggactac ctggagttgt
     9061 acttggttta ggagttacta taataacagg tatgggtggt tactttgtaa tgaaacttat
     9121 acgtgcagaa catccagcag taggtgcagc tacaggtaca acagccggaa atgcagctgg
     9181 tactcctgaa gctttagcag ctatagaccc tactctagct acaatagcag ctgtttcaac
     9241 agtacaggtt gctgctgcta ttattgttac agcaatatgt tgtccaatgt tagtttccta
     9301 tctagataaa agagaaaaaa agaaaaagtc tatttttaaa acaaataaaa atattgaagt
     9361 aaattaatac ttaaggaggt aaaattaatg aatgcaatta ttcatgcaaa tgaaaaagat
     9421 aatctagtta catgtgtcag acctttagtt aaaggtgaaa aagtttcaat tgatggaaag
     9481 tcttacatag taaatgataa tatacctgtc tttcataaga tggctacaga agatgtaaaa
     9541 aaaggtgatg ttgtatacaa atacggtgaa attataggaa ttgcaacagt tgacataaaa
     9601 actggtgact atgttcatgt acataacatt gaaagtacac gtggtcgtgg agataaaaaa
     9661 taggaggaag ttaaaatgaa attaatgggt tatttaagag aaaatggtca gtttggtatt
     9721 cgtaatcatg ttttaattat tccaacatct gtatgttcaa gtgagacagc tacaagaata
     9781 gcatcacttg tgcctggagc aatagcaatc cctcatcaac atggatgttg tcaaattggc
     9841 tctgatataa agcttacagc aaagacatta ataggatttg gaaaaaatcc taatgttgcc
     9901 gcagttttag tagttggtct tggatgtgat ggtattcaag caaaagaatt agcatctgaa
     9961 atagctacaa caggaaaaaa ggtagattac gttgttatac aagaatgtgg tggaacatta
    10021 aaaacagttt ctaagggagc tgaaatagta agtaaaatgg ctcgtgaagt atcaaaagaa
    10081 gtaagagttg aatttggtat gagtgaaatt acattagcac ttgaatgtgg tggttctgac
    10141 ccaacaagtg gaatagcttc aaacccttca ataggtgttg catcaaacct tcttgtagat
    10201 gaaggtggaa gtagcatact aagtgaaact acagaagtaa ttggtgcaga acatttgttg
    10261 gctacccgtt ttgaagatga agaaatgaaa gataagtttc ttaaatttgt aagtgatgta
    10321 gaaaaaagag caattgctat gggagaagat ttacgtagtg gtcaacctac tccaggcaat
    10381 aaagctggtg gactttcaac aattgaagaa aagtcacttg ggtgtatgta taaggcaggc
    10441 aataagccat tcaaaggtgc tcttgaatat gctgatattg tacctcctga taaaaaagga
    10501 ttatatttta tggatactcc aggtcaagat atagattcta tcactggtat ggtagcaggt
    10561 ggtgcacaaa ttgtaatctt ttcaactggt agaggtactc caacaggaag ccctatttca
    10621 ccagttatta aaatcactgg gaatagtgat acatacaaca aaatgccaga caatatcgat
    10681 ataaatgcag gtagaattat aacagatggt gctaaaatag cagatatagg tcaagaaata
    10741 tttaatgaaa taatagaagt ctgtaatggt aaacatacca aagctgaaag tcttggtcat
    10801 agagaatttg gcatttacag aatttcatct acattttaga ttgtaacaaa atatctttta
    10861 taaatataat acttatgtag atacataagc taaaagttct ttctaatact ataaaaattt
    10921 tctatactga aaaggaactg tatcaaaatt attttgaaaa cagttccttt tttaacctct
    10981 tttactcaaa atattataaa ataagattta aacaataaac gtatcttgat taatctctaa
    11041 gaagacataa ataaagctaa atgtaaagtg ggataaataa aatttaatat ctttgtatga
    11101 aaagatattt ggtataatat gtctgagaga attcttattt gtaaacaaat aaaggatgga
    11161 ggatgaaaca aaagaatgaa ctttgaaaac acaaaaaata aaaaaataat agctacttta
    11221 ctattgacac tccttttaat cgtgtcatta acatgtgtca cttttattat gaatctaaat
    11281 aaagtaacac ataatgaaac agaacgatat cttgtagaag tttcacaaag tatttccact
    11341 acaattaatg tgaaatgtaa aagtatattt aggacactat attctatggg agatacttat
    11401 tttgagatta atgatagtga aaaagaagtc aatagatact tatcaaaaaa attaaaatta
    11461 tttgaatttg attggttagg tattatagat tctaatggaa attttgtaag tgcccttaaa
    11521 gatacaggta atgttaaagg acaagacttt tatagaaaag cccttcaagg caaagaaaca
    11581 attactcctg ttaaatcaca atctggtaca tatggaattt tatattctgt accactatac
    11641 aatggcgata aaattgttgg agctgttgct gcttggaata ctattgatac tatgagaaat
    11701 ttattaagtg tagaaagctt tcatggtgaa ggattttccc aaattatcga ccaagatgga
    11761 aattttattg taagttcagc aaacaaaaat gctccaaaaa atgtggtaaa tttttttgac
    11821 atgattaaac accaaggaaa acaggttgat aaaaataaac ttgaagaaat gaaaataaac
    11881 ttaaaaaaga acaaaactgg ttctatgtat tatacattaa atgatggtat ttctaaggca
    11941 atgcattatc ttccattaca agaagaaaac ttatacctcc tttcaattgt tccaacaaaa
    12001 acagcaagtg cacagtttga tttacttcta aaaaaagcaa tcttcatcaa tattttaatt
    12061 gttatactat ttttattgct tattatattg attatgttta taaatcaaaa gaacagggca
    12121 caacttataa aaatcgccta tgtagactct gtaactaaag gttacagtaa agcacgtttt
    12181 gatattgaag ctaaaaaaat tatacagtca tcttcatcag aaagctattc attggtatct
    12241 ataaatattc agaaattcaa acttattaat gattcttttg gtagtgaagc aggagataaa
    12301 acattaaagt acatacatga tgtcattagt aattatctag aaccaggaga attgctttgt
    12361 cgagtttcta atgaccagtt taatgtactt attaaaacta ttcctcaaaa agaaattctt
    12421 aagtacatag aaaatatcac caatgatatt aatagtttca ataaaaatga agaatgtaaa
    12481 tattttatag ctctatctat aggtgtatat aaaattgatg acccaaatct acctttagtt
    12541 tcaattcaag accgtgcaaa tgttgcacga aaaaataata aaggaaacat tggaaatcat
    12601 ccactttata catgtgtttt ttatagtgat ttagagcgtt taaaaatgat taaagaaaaa
    12661 gaaatggaaa atagaatgga ggaagcatta aataataacg aatttatagt atatcttcag
    12721 cctaaaatca gcctcaaaga taatactatt gtaggtgcag aagcattagt aagatggcaa
    12781 gacccaaata aaggccttat accaccagac gagtttattc ctttctttga aaaaaatggt
    12841 tttattacaa aactcgacca ctatgttttt gaaaaagtat gtagtatgat aagaaaatgg
    12901 atagataatg gagaaactcc aatacctgtt tcagttaatt tttcacgtgc taatttaaat
    12961 aatatagatt ttttagaaaa atacagaatg cttcgtaata aatacaacat tcctgctaag
    13021 ctcttagaaa ttgaattaac tgaaacactt gtatttgaaa atctccaaaa attaattaat
    13081 ataatagacc aaatccatga agaaggtttt caatgttcac tagatgactt tggaagtgga
    13141 tactcttcac tcaatatgtt aaaagaaata aaagttgaca cattaaaact tgaccgtgct
    13201 ttttttagct cacctaatgc agataatatt gctgaaaatc atgtaataga atctatagtt
    13261 aatttggcaa aaaaattaaa tatgaatagt atatcagaag gtgttgaaac aatcttgcaa
    13321 atggattatc ttaaaaaaat taattgtgat atgatacaag gatatgtttt ttcaaaacct
    13381 ctacctcaag aaatatttga gaaaatgact tttggaaaaa aactagaaga aaccagtatg
    13441 gatagttccg tttaaataaa ataaaaaact tgtctctata aaataatttt aatcatttta
    13501 taaagacaag tctttttatt ttccaattta attttatttt ctaaatttct tacactcggc
    13561 ttgttttaaa ttcccttttt aagaataata atacgattat catagcaata aaatctgcaa
    13621 ctggctgtgc caaccaaaca ccatctaaat taaattgaat tggaagtaca atgattaatg
    13681 gtataaataa aataaattgt cttaaaagag ttaaaaacat tgaatgttta actttactta
    13741 tacactgaaa atacacaggt cctacaatag aaatccccat tataggcaat gtaattagat
    13801 ttaggcttat acctcttact gctatatcca tcaaatctga atctttatta aatataccta
    13861 caaatacttc tgggaaaact tgtactacta aaaatccaaa tgacaatatg acaactgaca
    13921 ctatgataga taatattaaa gttttctttg cacgttcata ttgttttgca ccataattgt
    13981 atgatattat agtttgcata ccttgattca atccaaacac aggcatcata aacatcaata
    14041 aaatagatgt aagtgctgtc atagctccaa tagctaaatc tccaccataa acctttagag
    14101 aattatttgt aaacatgtga gttatactta tagctacttc catagcaaaa ggtgtcattc
    14161 ctattgcaaa taccttcttt actatatctt tattcaattt taagttacat ttttttagtt
    14221 ttaaattgga cttccctctt ataaaataat gtattaccca aaagaatact actatttgac
    14281 agagtactgt tgctatagct gctcctttta tacctaaatt aaatacaaat ataaatatag
    14341 ggtccaatat caggtttaat acacatccta caatcattgt ccttgctgct aattttggat
    14401 taccttctgc cctaattgca ttattaagag aaaatgcaac taaattaaat atacttccaa
    14461 ccaatatgac actcatatag tcttttgcat aaaatattga atctttactt gcacccaaaa
    14521 taaaaagtat atcttctaag aaaactaatc ctaatatagt tataattaaa gagataatta
    14581 tagataatgt caaagcatta cctaatatct tctctgcttc ttctctattt ctttccccta
    14641 actttataga caatcttgtg ctagcaccta ctgataccaa cattccaaaa gctattatta
    14701 gagtaaatac tggcatagtt acaccaagac ctgcaatagc tatagagcca ataccttcca
    14761 tagaaccaat aaaagctcta tctactacat tataaagtga agttaccatc attgctatta
    14821 ttgcaggtat cgaataccta aataataaaa tccatatttt ttcatgtctt aatgcttctt
    14881 gattctccat gttgtcacca tttacaccat gtcataagtt atacaaattg gtttatttgt
    14941 tcttgggtct gttgctattt ctgcatcaat attaaataat tcttttagat tatctttcgt
    15001 cataacatca tatgcatcac cttcacaaac tattttacct ttcttaatac ctatcatgtg
    15061 gtcagcaaat cttgctgcat tattaagttc atgtattacc atgacaatag ttcttccttg
    15121 ttttttattt agttcatcta ataattttag tatctccaat tgatgtgcca aatctaaata
    15181 tgttgtaggc tcatccagta ataatatatc agtttcttgt gccaaagcca tagctatcca
    15241 tgccctttgt ctttgtcctc ctgataaatc ttccatattt ctatccttaa attctgcaat
    15301 tccagtaact tctaaactcc aatttacaat ctctctgtct tctttactag aattaccaaa
    15361 tccactttta tgaggaaatc ttccataaga aattaattct gaaacggtaa gaccactagg
    15421 agcttgaggg ctttgtggaa gtacagccat atcttttgct atttctttag acttcttttt
    15481 gtagatgtcc tctccattta tatatatttt cccactatta gggctaataa ttctacctat
    15541 agttttaagt attgtcgatt ttccacaacc attagcccct atgatggtag ttatttttcc
    15601 ttttggtata tttaaattta aattttttat aatatctgtg tttccatatg atacatttag
    15661 attcttagta tttatacagt tcataacaac accctttcct aatcatttac caacattaaa
    15721 taaataaagt acggcactcc tattatagaa actactatac ctactggtat ttctataggt
    15781 gctagaagat ttcttgatat agtatctgct attaaaagta ataatgttcc tatcaatgct
    15841 gatactggta ttaatctttt atgctttggt cctattattt ttctacctat atgtggagct
    15901 attaaaccta aaaatgaaat actacctgct actgctgtcg atacaccaga taaaattact
    15961 gcatatatta taagtcttct tctttgtttt tctacatcta caccaagact tatagatact
    16021 tcatcaccca aatttaaaac atctatatat ttagatttat aaatagttat acccataaaa
    16081 atcactataa atggaagcac agctattaca tatttccaac tggttcccca aatgcttcct
    16141 gatgtccatg ccataaccct attaaattct tgtgttgtaa atttcaattg gaatatcgtc
    16201 aaaattgaag taaaagctat gtttacacct ataccaataa gtagtaatct tgacgaattt
    16261 ataccacctt tccatgcaag agaatatata agaaatgctc caaataaagc tcctataagt
    16321 gcaacaatag gcattgtaaa tacagtcaag ttactcatac cttcatatac attaccattc
    16381 ataaggtata tataaactac tacaaacaat gcagcacctg aatttattcc caaaattcca
    16441 gaatcagcta aatcattctt tgtaactcct tgaagtatta cacctgctgt tgataatgca
    16501 gaaccaacca aaagtgctat tactattctt ggtaatctta atttaaatat tgcaatttca
    16561 tgattacgct gaccctgccc taaaaaagtc tttataacat ccattggttc tatagaaaat
    16621 gagcccatat ttaaactcaa taaaaaagtg ataaaaataa gcaccattaa aattgatata
    16681 acaagtaatg ttttagaatt tatttttata atatttttca tagtacctat ttctccccct
    16741 ttctaacaag gtatataaat attggcactc ctataagtgc tgttatagaa cctactggtg
    16801 tctcatatgg aggatttatc atcctactta atatatctgt atatacaagc aatacagctc
    16861 ctaatactaa agagctcggt attatatact tataatctga acctactatt cctttagcta
    16921 tttgaggaac tataagacct ataaaaataa tattaccaga cactgacaca gatgctccag
    16981 tcattaatat tacaagtact atacatataa atctaacaaa gttagttttt tgtcctaatc
    17041 ctattgccac ttcttctcca agacttaata tagttattct tggagccata ataatagcta
    17101 aaataaatcc aataccacca gctacaaata gcaattttat accttcccac ttcactccta
    17161 caagccctcc tgatatccag aaacttaatt gttgagatag gttaaaatac atagatattg
    17221 ctgatgccaa agaaattagc aatgtaccaa gagcgactcc tgcaagagct aattttacat
    17281 tattaactct tcctcttgat ttagaactta taaaatatat aaataatcca cttatacttg
    17341 ctccaataaa tgcaaacatc attacactaa aacttccatg tataagttga ggaagttttt
    17401 tttgaagtac cagtgatatc gaaattgcga atgtagctcc ttgtgtaatt cctataacag
    17461 atggttcagc tataggattt ctcgttattc cttgcattat tgccccagaa actgctaaaa
    17521 atcctccaac aagtgcagct gctattgccc ttggaagtct aacatccttt actattctca
    17581 tatttattcc atcttccatt tttatcaaac tatctataac agttgataga ttcatatctt
    17641 ttgccccaat agttatggat aatacaattc caaatacaag tacaactata cttactaata
    17701 tcaacaaata agttatattt tttttactca gcttagtcaa ttatcctacc tccttttccc
    17761 ttttaatcgt cagtataata tcatctatta agtattttaa ttttaagatt tattaaaaat
    17821 aggtgtgaaa aatgaatatc attatctcac acctactaag ttaagaatta ctttgttaat
    17881 tcatttttaa ctgattctaa taataactct tttccaattg gattgtatga ttgactgaaa
    17941 aatggagcag catcaagtat tgttacatta ccatctttta ctgctcttat ttgagcccag
    18001 atagcactat tttctaaatc ttttttatct gcttctgtag caattacaac tatatggtct
    18061 gcatctatat ctgttaaacc ttccatactt accattggta gtgttattcc atcctgtttt
    18121 ggcatattct taggtctagc taatttcata tcatcattga ttagagtacc tattccacca
    18181 tcactaaata ccataaattg acctgaactt gccaacactg gtaaataagt tttttctcca
    18241 ttcttttcta taacttcctt acctaacttt gtagcttttt catcatactt ctgtagccaa
    18301 cttttagctt cttcttcttt gtcaaataac tttgaaacat cagttaactt gcttctccag
    18361 tcatttgcat aatctttcat cataactaca ggagctattt cttttaattg gtcatatatt
    18421 ttttcttgtc tttgactcat tattattaaa tcaggattta cttctaatat agcttccata
    18481 tccatagtat ccatcattga gtgcccaact atctttacac ctttaagctc ttctcttatg
    18541 taacttggta atttatcagt ttcatatgag tctacatttg ctgttgcaac aggcttataa
    18601 cctgctaata atagttcttc actacttcct gatatatcaa ctattttttt aggatttgat
    18661 ggtatcttaa cctctccttt aacagattgt acaactcttg tctctgattt atctttatcc
    18721 tctgtattct tatctgaaca tgctgtaaca agtactaacg tagttataat agatataaat
    18781 attgcaagtg attttatttt tttcatgatt acttctccta cttgtttatt atacttacat
    18841 gatattgatt atcataagca catatttatt atagcatttt tctattttta gtcaatatta
    18901 ttttgtaaaa ggataaaaat tcctaattaa tcttaattat atatagctta gataaggttt
    18961 tcttatatta aacagtattt ttttaaagaa ttggacacaa atacaatgac ttttcagtta
    19021 aacaatgata aaatgcaatt tatagataat gaatttagta aggaggattc aaaacatggt
    19081 acatttagta tattgtgata atgcaggaaa aaaaggagaa aaggtcttag ataaaatctt
    19141 ggctggaaca aaaacaatgg ttattagagg tgctgctggt agaaaaatac cacatagtag
    19201 agtatttgaa gatgagttac tttattttat ggaaaaagga actgcaaaaa tatctgcaaa
    19261 agccactgtt aaaaaggtag aaaactatgt taaattaaca gaagatgaaa tttctaaaat
    19321 tttaaatgaa aatcaaccaa aattaaattt atctgaaaag caaaaagttc gttggcataa
    19381 aaaatgttta tgcttagttg aatttgaaaa tgttgaagaa ataacacctc ttgattttga
    19441 ccatcaagga aatatggatg attggcttat tgttgaaaaa attgaagatg tagttgttgg
    19501 tactagtata ccttacaatt atgagaagtc taaattttaa agtatcaata acataatact
    19561 atattgctaa actctactta aataaaaaaa gagtgcattt aataattata aaaattattt
    19621 ttatacactc ttttttaata ataaatttaa attacttact ttctaatctt tcatatacat
    19681 ctataaattc ttctgctaat tgttgaaaat tcatagctgt cattaaatgg tcttgtgcat
    19741 gaataagtat aacacttatt tcagttttat taccattagc ttcattttgt atcaattctg
    19801 tttggaattt atgagcttta tgaattgatt ctttcgactt ttcaatacat tctctagctt
    19861 tatctatatt cccgctcttt gcttctttta ttgcttcaaa tgctagccct ttcgcatcac
    19921 ctgcaaatcc aattattcca aaagatatct ctatcatctt ctcatccatt tattcgacct
    19981 cctaaatatc tctatagctt gatatgacta atccattctt ttctaaaaat tctttgactt
    20041 ttttagatgt aagtatcttg tgctctttcg ttctatcaat agcataagat gtagaattta
    20101 aaatataatc atctaaaaaa gctgggtgag acataatatc cactacatca tacttcatta
    20161 tttcgtctat attttttata aaatattctt cacttacatt ttccttataa aaactatcta
    20221 ttaaaggaac tactttagaa tactctaaat tatattcaaa tccacctctt attggaagat
    20281 tatacttggt tacaatcttc tctattactg gctttaaact tactaaagta tggatatgat
    20341 ggtgactatc taaatgagat atatctattc caagcccttt taccctatct atttgagcac
    20401 aaagttctct ataaatctca tcacaatcca ttttttcaat aatttcgtct gtaatcctcc
    20461 taataaaaaa accgtcttta tcaacaatag tttttaagtt agataataat ggtctaccac
    20521 aacttaaagt catatgaact ccacaattta aagttttatt ttctttaagt aagcctactc
    20581 catgttctac cccaggcata tttgccatca tagaagtaga cctaactata ccattattat
    20641 gagttgaaat tattccatag ttcacagctt cacaatatcc aaaatcatct gcatttatta
    20701 taattttagt cataccataa cctactttct atagacaact atctattaaa tctaggaaga
    20761 taatctttat gagcttccaa catttcgtct aatatagttt ttgctaagtc atcagatgga
    20821 atcaatgggt ttatacaaag tgccaaaaga gcagattcat aatcgccata tacagctgct
    20881 ttagctgcca atacttcaaa tgatttgatt tgacttacta atccatgcac agaatctggt
    20941 aaatatccta tacttattgg ttttggacca ttttttgtta ttacactact tacttctacc
    21001 gcttgttcat ctttaaaatc tcttatagaa cccttattta atgtattaac tacttggata
    21061 tcttttttgt cgttgtatat agaacttatt aaattacaag cagcatcact atagtaagcc
    21121 cctcctcttt tttcaagttg aggtggtttt acatctaatt tttcatcctt atataattca
    21181 aataattgtt cttccaattc tttaactact tgacctctag cttttccctc tttaaactct
    21241 ctcaactcat cctcaagcat ttctttagtt ttatagtagt atctatggta tggacaaggt
    21301 attgcaccca aagattttac aaattcagca ttaaaatcaa ttgcctttat attttgcata
    21361 cttatatctg ctttgacaaa tttatcaatt gcttcttttg taacatcttc tccatctaaa
    21421 gatacattta gaccataaac catatgatta agacctgcaa aatccatact tattctgtca
    21481 ctttctacat taaatagttt ggcaacatca ttttttaaat gaattggcac gttgcataga
    21541 cctatatatc ttctaaaatt agtatatttg aatacagcct ctgttattat tcctgttgga
    21601 tttgtaaaat tcaccatcca tgcatttgga cataattctt ccacatcttt tattatatca
    21661 aatataactg gtattgtgcg aagggcttta aataaacctc cagcaccatt agtttcctgc
    21721 cccataactc catgagataa aggaatagtt tcatctttta ttctcgcatc taaaagacct
    21781 actctaagtt gtgttgtcac aaagtctgca tctcttagag cttctcttct atctaaagta
    21841 agatttattt tcatatctac tccagctttt tttaccatcc tcttagctag atttccaact
    21901 atctctaatt tttcttttcc agcttctata tctacaagcc ataattcctt tacaggtaac
    21961 tcttcatatc tatttataaa accctctaca agctcaggag tataacttga acctcctcca
    22021 attgtaacta tttttagtcc ctttttcatc tcattatacc atccttacct ataatttaat
    22081 atactctaca aaaccctata catttattta ccgaaataaa actacctagt tgttaatttt
    22141 caatttatta gcttccattt tttcagacgc tatgacaaat ggaatatata ttaatattga
    22201 taatattaaa ttaaatagtg ctaaagctcc gccagtccag tcacctgtag ccattgctcc
    22261 acctaatata ggaggtgtta cccaaggtat tttaggtaaa attactggtg ctactaatcc
    22321 aaaatctatt gctaagtatg atacagcact acatattact ggtgcaagta caaatggaat
    22381 cataaatata ggatttaaaa ctattggaag tccaaatatc aatggttctc ctatgttaaa
    22441 tattgcaggt ggaccaccta atactatcat ctctttacgt cttcttccag ctattaataa
    22501 agctactatt aatcccaata ccattccaga accacctaaa tatacaaatg catcaaagaa
    22561 tgcacctgca agtacatggt atccttccgt tgctcctgct tgagccaagg cagcattttc
    22621 tccacctaat ttcataagta tagtttctgt aaaaggaagt gctatatttg caccatgaag
    22681 tccaacagtc caaagtatat gaattataaa tgcaataagc attgtagaac ctaaactatc
    22741 agctacattt gatagtggtg ctcctaaata agtatttaaa atatctgtta agaaacttcc
    22801 attagaaagc atttttataa atattcctat tacagtaaat atcattatag ttaacatccc
    22861 tggtattaac tttgcaaatg atcttgaaac tgctggtgga actccatctg gcatttttat
    22921 agttaatttc tttaccttag ataatcttac aaatatctca gttgataata atcctattaa
    22981 tatagaagag aataaagctg ctgtacctaa gtaggtttgt tgtatcattc cccatccttc
    23041 aactactgta gttcctccct caggaactat tttaccaatt tgtggagcca ttataaaaaa
    23101 gatagataaa gctataagcc cagattgtaa cccatcactc tcataagatt ttgctagaaa
    23161 ataagcaaca cctattacta aaaatactgc cattgtacct attgctcccc accaaaggtc
    23221 gcctccaaat gtggtccaat tttctccaaa tatacttgcc atcaaatttt tgtatgcttc
    23281 caatggtaag ttatttatca atgttcccaa tgcacctacc atagttatag gtatcattac
    23341 tataaatgca tctcttactg ctactaagtg cctttgagct cctattttag cagctacagg
    23401 cactatatat ttatccataa atgacatgaa tttttccatt ttacatcctc cagtacaact
    23461 ttattttgaa ctatttaact ctacagctct atctaacaca gcttttccat tacatcttcc
    23521 ataatccatc atatttataa cttctactgg aatattatat ggttttgcca tttcagttgc
    23581 ttttcctaat acatatctaa cttgaggccc taaaagtaag acatcacaat tttctatttc
    23641 attttttaag ttcacttctg atatagccca tatttttgct tctataccgt tttcctttgc
    23701 tgcatcctcc atttttgtaa ctaataaact agttgacatt cctgctgaac aagctaacat
    23761 tatttttatc attttttccc ctccaaatta gattcattaa atatttttta agtcataaat
    23821 ttttaaacta tacatttaaa atatgctttt ctattaattt tttcttaatg tatatatctt
    23881 atttttaaat gtatatacat ttaattcttg atattattat ataccaaatg tatatacatt
    23941 tcaagaaaac tttttcattt tatttaaatt ttattcgaca acagcattca aagttaatgt
    24001 atatccatag tgatatgtgt gtgagtactc aaaaatctca ctattttgta acttacaaat
    24061 ttggcttata tataaaagtg gctctggttc ttctaatttt agattatcca tacattcttc
    24121 attcgatatt acagcttgta cttctctctc tgacatggct atcttgtatc cacacacttc
    24181 ttcaacaaaa ttataaagag aattttcaca atgagtctta tttaaatttg gaaataactt
    24241 aactggcata taagtaatca tatatgaaac tcttgcatca ttagctaatc ttactctttt
    24301 tatttcccaa acattttctt ctaaacttat attcaactta cttgataaat tttcatctgc
    24361 ctgaactatt tcaagagata ccaatatatt agatacttta tagcctttac tttccatctc
    24421 tttagtaaat ccacttacag acgatatatt atcatatata ttatttgcaa taactatact
    24481 acctacacct ctttgtctag ttatatagcc ttctttaact agattattaa gtgcagacct
    24541 tacagtcatt ctagttacat taaattcttc actcagctgt ttttcagaag ggattaaatc
    24601 cccttctttc aaactgtctg aatttattaa ctctctaaca tgattttcta taactttata
    24661 aattggctct ttcatttaat tcactcctta ttaaatcttt tatataataa gataatttta
    24721 tatacaaaca aagaaaatgt ctatacattt aagataattt aaattttatt cagaaatctt
    24781 gtacaacttt ccgagtttta ataaagatat aaatagactt atcattaata taccttttaa
    24841 tatacttgat aaggttatac tccaccatac accatttaga cccaacattt ctggtctaga
    24901 tataaagtaa gccaatggta ctcttgcacc agtcagtata gtaattataa tagaaggtat
    24961 ataggttctc cctaatcctt tgaaagctcc tgttgttatt atttctaaac acataaaaaa
    25021 ttgagaatag cctaatattt taagataaac tgctcctttt tctattgcct cactttcatc
    25081 tataaaaata gaaaatatac tctctcctgc aaacactaat actaaagtag ttattatacc
    25141 aagtacagaa gatacagtta tagctatttt gcaaccttta tttattctag aatacttctt
    25201 ggcaccataa ttttgtccaa caaaactgcc taatgcaact gatagccctt cagccgtcat
    25261 ccaggaaatt gcttctattt gtgagcctac tttttgtact gcaacagcca caggccccca
    25321 tgatgctact attactccta atagcattga gaatactgta aacataccac tttgtattgc
    25381 tataggtaat cccaatttat acagaacttt ataatattta gtctctattt ctttaaatag
    25441 tcttaattta aaatattctt ctttactttt aactattata tatataaaac atgtagtaac
    25501 tactatctga gcaaatacag ttgcaatagc agctcctgca acaccaagtt ttggagcaaa
    25561 tccccatcca aaaattaata tagggtcaag tataatatta gttattaatc ctactgtatt
    25621 aatacgaaaa ggagttttac tatttccaag accattgaaa attgcagtaa atactggatt
    25681 aataaaataa aataccatac ctagagctac tattatcaaa tactgtctag acatggttat
    25741 aacttctaag tctcctaatc taaaaaaacc tatcaattgt ttattaagta ctattaaaga
    25801 aattgtatat attattgcta gtataatatt tatttctatt gctgacttca tatatgattt
    25861 agtagtgctt atattatttg caccaatact ttgtgctact tttatctctc cacctacttt
    25921 tgaaatcata ataaatgcca ttgcaagcca tggaaaaaaa ccagctgttc caactgcagc
    25981 tactgcttta cttccggctt taccaaccca tatcatatct atcatgttat accccatctg
    26041 tataaatgaa gtccccatta ttggaagtga tagcttaagt aatttttctg taattcttcc
    26101 ttctgttaaa ttaagtctgt ctttcatttt ccacctcttt tatattaaat ttatttgctt
    26161 atatagctag attaatcatt ttatttgatt gagttcacta tccttcaatt tatattatca
    26221 tatctcagga ttgcatctca atcatactta tgtttaagat acagtatttt acctatctat
    26281 gataaaatta atacttatga atataaatct agccatttca tcaatacata ttctaaccct
    26341 taacttaact tcaattaagt atacatatcg taaccaatta gaatatttta tacggtatct
    26401 tccattaata aacaaccaat atcaatgatt tatatataat tttatttata cataaaatag
    26461 tatactataa taataaaaaa actatatttg aatgctttaa gcatgtttac taattgtaat
    26521 ttccacacaa atttgataat atatgaatat attatggtga aatttataga tatatattaa
    26581 ttatggggag attgtgggag gttcttttca caagacattt aagctatgaa aataaataaa
    26641 aaattcaata gagttgatat gcaggtttca atacttactg caattattgt aataatatct
    26701 tcctcatgtg tatatttttt taattataca ctaacttata agagcatgat atactcttta
    26761 agtgagaggt caaaacatat ctatgaatat gtagaaagaa atattgataa aaataccttt
    26821 actgaaatta attctaaaga agaccaatca aaaaagtctt ataaaagttc taaaatcctt
    26881 ttagaatctg taaaaaatac tactggagtc atgtatctat atactgctaa aaaaacaaaa
    26941 gatggatcac tagtatatgt tgttgatggt ttaaattcat ctaggagtga ttttagaaat
    27001 gctggtgatt tgatagaaaa agagatttgg ggagacctta acaaagcatt agataatcat
    27061 atattgttac ctaaaaagat taaatcaaca tcttggggat acatatttat atcgtatttt
    27121 cctattcaca atgatgttgg taaggttgtt ggagtaatag gaattgaatt tgaagcaaaa
    27181 catcaataca atacatttaa atttataagt atagttacac caataatagc attattaact
    27241 tgtctaatat cagcattgat tgcagtgatt ctatttaaaa gaatatctaa tcctacatat
    27301 actgattttg ctaatacaga ttatttaaca ggcctaaaaa atagaaatgc ttttgaaata
    27361 gatatgaata acttaaacaa ttcaaatcta aagaatctaa tatctatcat atcaatagat
    27421 ttagacggtt taaaaaagat taatgataaa tttggccatc agacaggaga cttatatatt
    27481 aagcaagcaa gtaaaattat acaatatgta ataaataaaa aatatatagt ttatcgtatt
    27541 ggaggtgatg aatacataat tattctaaaa aatttatctc acaaagaaat aaataatatt
    27601 ataaataata ttaatttaaa aatagttgaa gaaaactcaa ctaatgaatt aaaattatca
    27661 atgtctattg gatatgctat ttttgatgaa aaagtagatg tttctttatt tgatacatat
    27721 cgtcgtgcag attctcaaat gtatgataac aaaaggaaaa ttaataaaca atatcaataa
    27781 taaatttaaa aagtctattc tttaaataat aaatcacaag aatagacttt tttaaatttg
    27841 tgtttttata agataaatta tttaattaaa gtttgttctt ttttctaaac gattctttat
    27901 aaattcatat attactttca ctaatatatt tgtgactaat attaacacag ctagtgctgc
    27961 tgcctgtgca gtactcccct tatcatctag ttgtacaatt gatattgcag ctacttttga
    28021 acttgcagta tatataaaaa ctaatgctga tattgtcacc atggagttta ggaaaaagta
    28081 cataaagttc tctaaaactg ctggcaaaca aagtggtata gttacgttct taagaattga
    28141 tgcgctgttt attcccattg atttagctac catatctaat tctttatcta ctttcttaat
    28201 agcagtagta gctgttataa atgctactga aaagaaatga actatattac atataaccat
    28261 tataagtaat gttccatata atccattaaa caaattctta atgtatattg tatccataaa
    28321 attaaactct aatttattaa aaaataatac ataagctata cctaaaacta atcctggcaa
    28381 tgccataggc aatgtactta aaaaataccc aaatgatttt aaatatggag ctttttctgt
    28441 tttttcattc aaatatgcaa ataagaaaac aaagctcgtc cctagaatag ctgtaaatag
    28501 tgacattttt attgaattta taaatggttt aatcccatca atactgtttt tcatagaaaa
    28561 atgttccagt gtcagtctta aatcatatgg ccaaactgat attaatgaag ataagattac
    28621 agttaacatt acagctatta taccaaatgc aataattcct acaacccctc ctaaaatact
    28681 atctcttaac ttattagttt taaccttata tggaattgac tttgaagtta tatatgaatt
    28741 ttgatttttt tcaatttttt tagttactat aaacgatatt attgcaggta ttaaaagtaa
    28801 tattgataca gttgctccca ttggcatatt ctgttgccct ataatttgct tatatacatc
    28861 tattgataat acattaaact ctcctcctat cacctttgga gctccaaaat ctgtaaaaca
    28921 taatgtaaac gatacgaatg cagaacttat taaagagtat tttatacttg ggattgttat
    28981 gcttataaat tctcttatct tgcttatccc catagtatta ctagcttcat atagtctata
    29041 atcggttgaa cttagagata tataaagtat ttgaaatgat tgaggaaata catatataat
    29101 ttgtgccaat attattccca ctgctccata tattggaaca tgcactccaa gtaaattagt
    29161 taccaaacct tgatttccaa ataaatatac tagacctatt gcatgcatca ttgttggagc
    29221 aaacatagga aacatagcta atgatttaaa aaaaccttta aatttaatct ttgttctagt
    29281 taatgcatat gcatatacta atgcaagtgt tagtgatatt acagttgata taactgatac
    29341 aaatactgta ttttttagag aatttaatag agattcagaa gccaagtatt cttgaaagtt
    29401 ttgtaatcct acaaagactc catctttatc taaaaaagcc tgtacaaata gatatattat
    29461 aggtgctaat aaaaatatta ctaaagacat aactaatact gtaagcatac atttttttat
    29521 atctaaattc atttttgatt taagacttat acttttagaa cttatagcat tattattcat
    29581 acacttatgc tcctttaact tttggatact ttaaatagta tttttcgtct atacttattt
    29641 ttacaaggtc accttctact aaatttaaat taacccactc ttttatagaa acatcactta
    29701 ttatgaaatt ttcatttaat ttatctttta tttcaactct caataaattc cctctaaact
    29761 ctatatttga tattatccct tggtagtttt cttttgtcga tttctctatt tgaacatatt
    29821 ctggtcttac agttaatata gaatctccat tatcaaaaca gtttgtatct cctataaatt
    29881 gagctgtaaa taggttttga ggattttggt atatttcttc tggagtccct atttgcatta
    29941 tatctcctcc atttagaata gctattttat ctgccatagt tatagcttct tcttggtcat
    30001 gtgttaccat tattgtcgtt atattaagtt ctttttgtaa tcttttaatg tccattctaa
    30061 gcttgtgtct aacctttgca tctaatgcag acattggttc atctaaaagt aaaaactttg
    30121 gttcaagagc taatgctctt gctattgcaa tcctctgttg ttgtccacct gataatgctt
    30181 tagggtattt atgagcttca ttagtcaatc caactgtttc aagtacttcc ttaactttat
    30241 tatctatttt ttcttttgaa acttttctct cttttagtgg aaaagctata ttgttataag
    30301 cagtcatatt cggaaataat gcatatgatt gaaatactat accaaaacct ctctttgatg
    30361 gctctaaatt tgtaatatct ttatcctgga gaattatagt tccactattt acatcttcaa
    30421 gacctgctat tattctaagt aatgtagttt taccacatcc actaggtccg agtaaacata
    30481 gaaattcccc ttcttcaata tccaaactaa tattatttaa aacctttttt tggtcataac
    30541 ttttaaacac attatttatt tttaagtaac tcatatctta tctctcctta tatagttatt
    30601 tttttatttt aaattttata tcctactttt tcttctaaaa ttctttcatc taaattatta
    30661 atttaaactt tacttacttt cagattttga accgtatttt gattcccaag ttttaagtac
    30721 tgtacttctg tttttggcag cagagtttaa atcaacttct tttataagct gttcaaatgg
    30781 ttgtttagaa taaccttttg gaactggatt atctgtcttt atggaagtaa ctgctacttc
    30841 atgtgcatat ctatccatag ctgaatcact tattgcccaa tctaaaaata gctttgcttc
    30901 tttttttata ttatcttttt taactaaggc atttgcttct aagtcccaac ctgaaccttc
    30961 ctttggaaat acaacctcta taggttctcc actagataat tgttgtgtag ctctatatcc
    31021 aagtgtaata cctattggat actctccaga gccagctaac ttcgcaggtt ttgaacccga
    31081 gtgtgtatat gtggctatat ttttatgtaa tttatccatg taattgtaag catctttttc
    31141 acccatcatt tgaataagcc ctgatactgt aagatacccc gttccagatg atgctggatt
    31201 tggcattgtt ataagtcctt tatattctgg ttttattaaa tcttcataac tttttggaat
    31261 atctatacct agtttcttaa gttccttata attaactact attgctgtct ctgggacact
    31321 cattcctacc caagatacat catcttcagt atctttaaat tttttatcaa ctttatcata
    31381 atttttaggc ttatatgctt caagtgcacc atcttttttt agctctatca tgttcattgc
    31441 tgataatccc catataatat ctgcttgtgg gttatctttt tctgctaata gttttgaagc
    31501 tattacacca tgagaatcca taactatctc tacatctata ttgggatact cttctctaaa
    31561 accttccata taataagcta tctgctcttc ctctagtgct gcatatattg taagtttatt
    31621 atttcctttt ccttgagtag tttgtggtgt tgttgatatg caacttgtca taaatgtaat
    31681 cactacaatg cacagacaag taaatataaa ttctttcata tcttccccct ttgtgttttt
    31741 cccttttttt atttgttttt tattgttttt gcttatgttt atataataca cctaatttgt
    31801 taatataaag ttaagtgtaa attaacagtt cgttaatttt tagaaatttt tgttaaatat
    31861 attattttta gttaaataca gtctgttttt aaacaatata ttaattttta tagtatctac
    31921 attttaattt aaacataaaa aataagtcta acctctataa agtagaatgt tagacttatt
    31981 tttataagta tttaattata caaccaaatc attttttttc ttttcttcaa gtgctttttt
    32041 aagcaccata tccttaacct tcataagtct agtagtactt gctctctcat tttcttcaag
    32101 cttcatagct atatacttaa tagtctcaat gtaatttggt atcattacat tttcaagagc
    32161 attaacacgt ctacgtgtct tttctatctc ttgtgcaaga agctgtgcac ttttttcact
    32221 ttctgcaagt cttaaaagtg gttgcatagc atctgagaaa gcttccatag cagaatctaa
    32281 ttcacctgat gtaaaagcaa gaccataagg ataaatatca ctttgattgt tttcagtttt
    32341 aaagtcaaac acaggaacat caacactcat tatatttctc gtagaaacat ctactgaaac
    32401 tgattgcttt ggcatcataa gtgctgaacc cagatactcc tgactcataa ctgctcttgc
    32461 gattataaaa tttttataag cagtatcaag agcattttct gcttcttcac gcaatcgctt
    32521 attttctctt acaatctcaa gaaattgttt cataagttca tcaagtttat ctttaagaag
    32581 cttatgacct cttgtagcag ttttaaggag tttcttaagt ctagtcattt ccatacgtgt
    32641 tggatttata tttaatctag ccacagatat cactccttag gcatatattt ttcaaaatat
    32701 tcatcacgga tacgtttaag ttcacttttt ggtaacatac taagaagctt ccatccgatt
    32761 tctaatgtct gctcaatagt tctatcagtt ctaaatcctt gtgaaacata ttctttctca
    32821 aattcatctg caaacttagc atacattaaa tcaatctcag aaagtgcaga ttcaccaagt
    32881 atagccataa gttcttttgc atccttacct cttgaataag ctgcaaaaag ctggttcatt
    32941 gtatctgcat ggtcttcacg agtcttacct ttaccaatac ctttatcttt tagacgagaa
    33001 agtgatggta aaacatctat aggaggctgt atatcttttt tataaagttc acggctaagt
    33061 ataatttgac cttctgtaat gtatcctgta aggtcaggaa ttggatgtgt cttatcatcc
    33121 tcaggcattg taagtattgg aatcatagtt atagaccctt catgcccttt catttttcct
    33181 gctctttcat acatagtagc aaggtcagta taaagatatc ctggatatcc acgacgtcct
    33241 ggaacttctt ttttagctgc tgaaacttca cgcaatgcct ctgcatagtt tgtaatatcc
    33301 gttattataa caagtacatg catatcttgc tcgaaagcaa ggtattctgc tgctgtaagt
    33361 gccattctag gtgtagatac acgctctaca gctgggtcat ttgcaaggtt tacaaacact
    33421 acagaacggt cgattgcacc agttgcttta aaatcgctta taaaaaattc agcatcttca
    33481 aaagttatac caactgcagc aaatacaacg gcgaacttag aatcagttcc acgtactttt
    33541 gcctgtcttg caatctgaac tgcaagattg gcatgtggca aaccagaccc tgagaatata
    33601 ggaagttttt gccctctaac caatgtatta agtccatcta tagcagagac tccagtttgt
    33661 ataaactcgg ccggataatc acgtgctgct ggatttattg gagctccatt tatatcaagt
    33721 cttttatctg gtataatttc tggacctcca tcaataggtc tacccattcc actaaaaaca
    33781 cgacctaata tatctggtga aactccaaag tcaagactct ttcctaaaaa acgaacttta
    33841 ctttcagcaa ggtttatacc tgttgagctt tcgaaaagct gaacaagtgc agtatctcca
    33901 tttacctcta gaactttgca acggcgaatt tctccatttg aaagttcaat ttctccaagt
    33961 tcatcaaatt ttacaccttc tacgccgttt attaacataa gaggaccggc tacctcttgt
    34021 attgacttat attcctttat catagttctt cagcccccct ctttttaata aggtctgcaa
    34081 gctcgttgtc tatttcatct tcaattgaat cgtaagctgc atctatatcg ccttcttcta
    34141 catacttagc acgaccaatc ttttcaagaa ctgaaagctt gataatatcg tttatagtaa
    34201 cattttgctt gatagcatct tgtgctgact tatagaattt taatataagt cccatcattc
    34261 tatattgttt atgaagtgat gtataagtat caacttcatg gaaagagttt tgatgaagga
    34321 agtcttctct tatagaacga gtaacctcta gtatcaatct atctccatct gaaagtgaat
    34381 caaccccaac caattgaact atttcttgaa gttcagattc aacttgtaaa agtttcatag
    34441 cttgagctct ttgagctgag aaattatcat ttacattttt atctgcccat acatcaactt
    34501 tttcctgata gagtgaataa ctagtcaacc agttaattgc tgggaaatga cgtttatatg
    34561 ctaaagatgc atcaagaccc cagaatactt ttactatacg aagcgttgct tggctgacag
    34621 gttctgatgt atcaccacca ggaggagaaa ctgcaccaat tgctgtaagt gttccttctc
    34681 tttcatccat acctagacaa tttacctttc ctgctctctc atagaattga gcaagacgtg
    34741 aacctaagta tgcaggataa ccttcttcac caggcatctc ctctaaacga ccactcatct
    34801 ctctaagagc ctcagcccat cttgatgtag agtctgccat aagtgcaaca ctatatccca
    34861 tatctctaaa atattcagct attgtaatac cagtgtatat agaagcttca cgtgcagcaa
    34921 ccggcatatc tgatgtgttt gctataagca cagttctttg cataagtgac tcgcctgttc
    34981 ttgggtcttt taattcagga aattcaagaa gaacatctgt catttcattt ccacgctcgc
    35041 cacagcctat atatacaact atatctgcat ctgcccattt agcaagctga tgctgtgtaa
    35101 cagtttttcc acttccaaaa ggtcccggta tagctgctac cccacctttt gtgattggga
    35161 aaaatgtatc tataactctt tgacctgtaa caagaagtga atctggtgtt ttcttttctc
    35221 tatatggtct ttcttttcta actggccatt tttgcatcat agttacaggt atatcattac
    35281 ctttttcatc tgtaataaca caaactgtat cttcaacagt aaattcacca gaatatattt
    35341 cttttatagt acccttaact ccataaggaa ccataatttt acattctact actgctgttt
    35401 cttgaacgcc accaattata tctcctgcta caaccttatc accaacagat tttttaggtt
    35461 taaattccca cttagttgtt ctatcaagtg aagatacttc aacaccttta gttatattag
    35521 tacctgaaat atcatacatt ttttcaagtg gacgttgaat accatcataa atagttgaaa
    35581 taagacctgg tccaagttca acactcatag gcattccaag agaaactact tcttcccctg
    35641 gtcctagacc agaagtttcc tcataaacct gtatagaagc tttatctcca tgcatttcta
    35701 taatctcgcc tataagatgt ttatctgaaa ctcttacgac gtcaaacata ttcgcatctc
    35761 tcatgccctc cgctacaaca agcggtcctg acacctttac aattgtgcct gttttcatat
    35821 tctctcacct gcaatcatat actttatcaa aatttaaata ttccctccta tattttatta
    35881 gcacatatat ttagcaccaa taaaatttga gattgtcttt tattcaactt tcatctattc
    35941 tttaaataat atatctgcac ctatagcacg tttactagat tctcttactt cattcatacc
    36001 taatcccata cttcctccaa tacctggaat aactataatg gcaggaagtt ggtaatcttt
    36061 gtattttgct attgattctt ttgcaagcaa tgatgcctgt tctgtaatat aaattatcgc
    36121 atattcatct tcaaccaact tatgtatact ttttttaatt tcgtcactat cataggctgg
    36181 gaagatatct attccaagag ctaaaaatcc catgatgcta tctttatctc caactactcc
    36241 taccttatac ataagcatcc cttagccttt cttttatagt atctgcgtct atattattaa
    36301 gcttactagt caaaataatt ctaattgttt ttatctctga ttctttagca tataaatatg
    36361 caattagtgg ttctaatgta agaggcttaa attttgctcc tctaacatat tccataagat
    36421 agtcatcaca aagtttttca aagacagtaa aaccactatc catgccattt ttacagacat
    36481 cagagtaaga agtagattta aaacatgatg ctggagtatc tgaggaaaaa gcttctaaga
    36541 atttttcctt atcaagtgag ccacctgaaa caaacacatt cataaacata tcaaaatctt
    36601 tcttcatatt tttgactctt ataaaactct ttagatttgt taaatcacaa actttcataa
    36661 cataatctat tacaaatttg ttttggcttt ctttagcagt ttctttcata ctggtaaagg
    36721 ttgctttatc tagaacaata tcaaccatct ggccattttg tgtcttatta taaacatcaa
    36781 atgcctctaa tatagctgat gacattatct taggtaaatc actaaaacta cgatttgcta
    36841 aagattctct aagcttaata agaggaattg tacctccatc aattaaatat ttctcaccat
    36901 ctacaccaga aatttcttct ttaatcagca cttttaaatt gtgatagtca tttttataaa
    36961 gaaatacatc aacaaatttt tcttcaggag ctagactttt aagaatttca tatgtctttg
    37021 ataattcttg agtcaaaata ttctcaaaat tatgtatatt attatttgat gtatcagcat
    37081 aacctgcctc agctataatt cttaaagagt cttcagggct tttggcttct gccatttgaa
    37141 taaacatttg tctgttaaga agctgttttt caagcactct tattcgtgca acggcatagg
    37201 gataagtttt ttcttctgcc attttttaac ccctccttaa ttaaacaaaa tttctgctgc
    37261 aatttgttca atatattcat gctctactgc aattatggct tcaaatgaat aattgtactc
    37321 aatgtcacct tttttaacta tgaaacctcc agtaatatct ctagtttcat cagacacttt
    37381 aatgcctttt tttgatagca catcttttaa tgttttttta tcttttttag ataatactat
    37441 ctctgaatca tctgtacaat ttgatttttc aatcatgttt agtataatct cttcatattc
    37501 attatcttta agatttaaaa gtttgttttt agcttctaaa attacctctt ctaaaatctc
    37561 ttgttttttt gataatattt gctttttagc ctccatataa gctcctgata tttccttaga
    37621 agccgctttt tctgcttctg cctctgcaag ctttgtatag gaagccattt ccttttcagc
    37681 tttttcatta gctgaattta cttttaaatc tgcttcactt ttagctttat ccaatatttc
    37741 ttgtgcttct tgtttggcat cagctataat tctgtcaatc attttttgtt cattacccat
    37801 aggagtgctt cctccttttc atttttttta cttatgaatt ataatttata attaaccaat
    37861 tgcaattcca ttgtaagcta agaaagaaac aagtagtcct aaaagagcat atgtttcaac
    37921 tattgctgca aatgttatag ctttaccaag ttcttcaggt ctttttgcaa ctattccaac
    37981 tcctgctgct gctgctttac cttgtgctat acctgatact aaaccaacta aaccaattgg
    38041 aagtcctgcc ataagaagtt gtaaaccttg agctgatgtt ggtattgcat ctccaccaat
    38101 aattcctgct tttgataaaa taaggaaagc tatgataaat ccatacagac cttgtgtacc
    38161 aggaagaagt tgtaaaacta gaagttgacc gaatttacta gggtcttctg ttacaacacc
    38221 tgctgcggct tgacctgcta ttgaagtacc tttagcagaa cccatacctg caaataatgc
    38281 tgctatagca gctcctgata ttgccaaaaa ttgaccattt cctaacgcgt ttaaaaattc
    38341 catatttaca tctccttttt attaatgctt gtatattttg ttttatactt aaacggaaca
    38401 aaaggaactc ctccgccttc ataaaactta ttgaaaaatt ctacatactg taacctactt
    38461 gtatgaacat atgcacctag tgcatttata agaaggttta ttgtgtgtcc tacaacacca
    38521 attacaactg caagtatcgg accagctata gcccctaaaa ggttgataac ttgtgctata
    38581 actcctgttg ttaaacaaag cgccataatt cttgtataag ataatatatc agcaaaataa
    38641 cttgttatac catagagact tgaaaatcct ttaaaaagct taacaggaat acctttaaag
    38701 cttcttcctt gagtaagtac aagacctata gcacctataa ttgctaaata ttttcctact
    38761 tctgaaaata cacctatgtc ccctgcaaca aaaggtatta taagtagaca tacacctgtt
    38821 atacacaaat accaaaaacc tatgtcaaag attgcatcta ttacctttcc acttcttatt
    38881 aatgtatatg cttttattcc caaacctaca tatatatgga taataccgaa cagtaacgac
    38941 attcccataa gtgtcataac atcctctaaa ggattaatta atgcttttat aggaattaaa
    39001 tcccccaaaa tacccccgaa tataagaccc catacagtag ttgaaactcc acagatacca
    39061 ataagtttaa ttaaatttcc ttcacctttt tgtatcttta atttatatac aacaaatcca
    39121 caaactactg caattattaa cccatatgcg gcgtcactta gcatcattcc aaagaatata
    39181 acaaagaaga aagttaaaat tgtatttggg tcaatatctt ttgtacttgg atagctgtac
    39241 atgttagtta cactttcaaa aggtgttact aatttattgt tttcaagcag aattgggaag
    39301 ccttcttctt tacttccttc ttcagtctga acacaacaat caaacttttc tgtaatttct
    39361 ttaattaatt catctgccct tttgcttggt aaccaaccat taagacagaa tgttgttttt
    39421 gttttgacaa gtctctctat aatctttttt tggtctcttt ctatagtgta aaaatcatat
    39481 aggttttcaa gcatgtgtat actatctgca tgtcttttaa tttcctctgt tttgtcctta
    39541 gaaacgtttt cctgtctaat tatcatttca ttgtatttct tgatagcttg taacggtgta
    39601 ccttcttcat ttggtaatga aaccactgaa aaattaaatt cttttaaaac ttctaaaaca
    39661 tcatcaaaag tttcttttaa agtaattaaa taaacatagt tcattaactt gtcagaattg
    39721 acaatcccaa taacactttc tggtatttca tcttctaatt ttactcttaa agcatcaatg
    39781 tttgcgctta tgggcacagt tccaagtata gtttttgtat acttagtttc catattttca
    39841 ataggaatat caaagttcac ccatggttct aacatacttt tattgttgtt aagtaagttt
    39901 atgttagacc ttattgtacc tatctccttg tgcaaattgt ttaactccag agcttcagca
    39961 taaacttgcc cttctttttc actatcaatt ttgttaaact ctctttttgg tgcaagtagg
    40021 gatttttttt gtttcactag tgaagaaaca atattaattg cttggtttac atgagacatt
    40081 ttctgctcta gcattataac ttcagattct tcactatctt ttacaagata attttttaat
    40141 tcctcctcct cagtaagaaa ggcactatca tcaatctgaa caaacccacg gttcataagt
    40201 agctttaaaa tctggctttt ttgagcttct aagccaacaa tagatatttt gttcatatta
    40261 actattgcca tattatctca ctacctttcc aataaccgct tcaatagcct tactttttct
    40321 ttcttcagca gttttttcaa ttttcagaca ttcactttta gcattagcta agattttatc
    40381 aacctctgct ctactgtcgg tgactgcctt ctcaataaga gtttcttcca taagctttgc
    40441 ttgttttttt acatcagcaa atatgctttc acatttgttt acagcatcag ctctgataga
    40501 ctctgcatca gtaacagctt ttactttgat actgtctgct gtctgctctg cctcaacaat
    40561 gctttttatt atttcaaaag ccatccaaat cacctctctt acctttttac tatgtatata
    40621 accaattaga tttttttatt ctaattagat tactacctca ttgttaaagt ataaacagca
    40681 tttttatata ataattaata tttttcaacg tttatttctt aataaaacat attttagaat
    40741 agtacttgcc tagatttaca tccttttttg acttactttt agactatgac atgagtatgg
    40801 tttttaaata ccattatttt caataattat aatttaaagc gatatagttt gtcaatagcg
    40861 ttattttgtt ttaaaattaa cgttatgtcg acatttcaaa tttttatttt atttttttta
    40921 taattttaat ttaatttatt acatataaaa gttttttata ttctttttca acctctaacc
    40981 attgaagttt attgtttttt ttgaattttt agaaatatct cgcactattt tatctaactt
    41041 ttataaatca tcaagctata tcgatttttg tcaccttatt tttaatctaa tttttttata
    41101 tctagttaga aataaatcaa tatttttaaa gattgataaa acttacacct agttctaata
    41161 gttttatttt aaggcaatgt attaatctta agtagataat atcattagtt actctatata
    41221 ttcgacaaca ttataaaaaa atcctcgtac caatcactcc aatcttcaat aggatatgct
    41281 gctcctccat ttacactttt cagtagattg gatgtatatt cttctcccat agaaaaaagt
    41341 tcccctactg tcatatcata acctctagat gtaacaacct tacaaaatga atccataaca
    41401 tcacgttgtt ctcttgtttt tataagttcc tttctaaatt catcatcatt cttagcttct
    41461 tctaaaattt tagttaattt ttcattcatt atgtatttct ccttttaagt agtctttctg
    41521 taaacttcaa taagctttat ataaaaagga agccatccta aataaactaa gatgacttcg
    41581 cttaactgtt agcaacacat ttactagtat aaaataattg gtgtacctgg ttctatattt
    41641 tcatatattt tctttgcctt agaaaaaggt gtatttacac atccatgtga acctgaagac
    41701 ttatatatac ttccaccaaa agaatttctc caagtagcat catgaatgcc tatgttccca
    41761 ttaaatggaa gccaataact tacaggtgaa ctgtaacctt gaccagttaa tgaaacattt
    41821 ctagccttat aattaagagg atatatacct gatggtgtag caaatcctct agctaaatca
    41881 cctgttacaa cgtctgttga tacaagcatt tctccatttt taaagaacca catatgttgg
    41941 ccagaaagac taatctctac atatgtattt ccaatatcat ctttattacg agatacagct
    42001 ttttgtatgt aaataggttc tcttgtaaca tttttaccag cttttacaac atcaactaat
    42061 gcttcagctt ctttcttttt atctatcaac caaccataaa ttccacctga aattgtaata
    42121 tctttacctg tggcagtttt aaatggtctt gagtcaccaa aagtactgta ttttttactt
    42181 aaacctcttg tatattctac catagcatca ttatcaaaag ttatgtcata attcttttct
    42241 gagtctactt ttaaccattt attaatttca gaaccatcta aagtttcctt acggtcacta
    42301 aaatcatatg taacgactac accaacatac ttatctaatg tcttaatagc ttgtttcaac
    42361 ttttctgaat cctttgtgtt gtttggtgct tcataaactt tactttcatc cagagaaata
    42421 gaagtattcc cagaattaat tgagttttgt actaacttat aaaaatcatc ttttttcact
    42481 ttgtttccaa gtacctctgg tactataaca taagcgtttt tagtgctatc atacttagga
    42541 taagcactct taggttctac aacattttta ctatcaaagc aagatagagc gttaaatctt
    42601 ttttctaata aatctttatt atatgaaaga ttcaccatat tattatactc tttagactta
    42661 aatattccac caatccatcc aatagcgctc tgactatctt ttatcttttg aactttatca
    42721 tctggtgtaa attttaaatc aatatctttt gcttttattt gttctttctt attgtttctt
    42781 tcctctaagt ttaaagtata ggaatttgta ctagatgtta acaaatcatt tacttgctca
    42841 actgtttttc ctgaaacctt tacgccatta atagtcgttc caaagtaaaa atgatttcta
    42901 aaaaagattg ccattcctat ataagctaca aacaaaacac ctactactat agctatctta
    42961 aattttagat ttttttgtcc actttcttgt tttacctctt ttccatcaat cataattaaa
    43021 tccttcctta cattgtaatt acctatgtac cttattggtt tttaaacaaa accatgtatg
    43081 ccttatttta tatacccaaa ttactgtatc attatttata tttttaaata tatagttatt
    43141 tccataaaac cattttatct tttagataaa atgattatta taaatccccc ctttcaaaat
    43201 cccttaaaca caatctctgc tatattaata tattaccata cttttaataa tcgtgggact
    43261 atctatctat ttttgtaaca ctaaactact cttttaaaaa agaaactaat tttgtgttag
    43321 tttctttatc ttaacttatt tttatattga attttagctt aacataacgt tgttatatag
    43381 attctattta attattttag tttctatatc ttttccatct gcatattctt taagtaattc
    43441 cttagcatat ccaaaatatt cttcaatatc agattcttga ctaaaagaaa atatacaaat
    43501 taatatttca tttgcatctt ctgttatcat tgctctttca gaatcactag ttggactccc
    43561 aaatttaccc actgaatcag acaatatagg aagattttct atatttatta attctttccc
    43621 aataccctta tactgttctc cttcatctcc tacagtaaaa catactggtg aatgtaattt
    43681 gcttacatta tatgtaccta ctggatataa tgattttatt gatattaaat tgtttatttc
    43741 tactatatta tttattgtat aaagaccctt cccttgaata attcttctaa ccaatgcttc
    43801 tgaagatgtt ctatatctac ttggattttt tccaagctct ttataagcat ttcttgcatc
    43861 tttaatttta tctagtttcg taatgtcttc aacttttaga ctttgattta tagtatcaca
    43921 aaactcattt atttcattaa ttaatttttc tgagctttta tttactgcta cttttgcttg
    43981 aatgcaaccc agcttagcat taggccatct ttccttaagt ttgtcatcaa tacttacatc
    44041 tatcatactt aaacaactcc tatacactaa atttttatta aagtttttat catctataca
    44101 ttatagtact attcttttgt taataacatg tttattagca aaataaattt atttttaata
    44161 taatttttat aaaatacaat tattttttgc tttatattag acaatacatt gataaaataa
    44221 aaaaacacta attatttagc aatgatttat taaaatcaag cattcataat agtgctttat
    44281 ataaaaatca aattttgttt taaagtaaat ctaaaaaatt cttagctggc attggtcgtg
    44341 caaaataaaa tccttgaact aaatcacatc caaattgttt cattaactca gcttgttctt
    44401 tactctcaac accttctata attacaacta aatctagtga ttttgctaga gacactattg
    44461 attttgctat attgcgactt ctctcatcat gttcaatgcc accaagaaaa gacttatcta
    44521 gtttcaatat atcaattggc atatctctta aacaactaat agaagaatat cctgtgccaa
    44581 agtcatccat agaaatagaa aatccatgct tcctcaattc atttaaaaca ttctttaaaa
    44641 tatttaactc attaatagcc acagtttctg ttatctctat ttcaatgaaa tctgaactta
    44701 gattatacat ttgtattgtt ttattgataa attttacaat atctggttga tacaactcga
    44761 ctctagataa atttactgaa actggaacta tctttttacc ttgctttttc cattcagata
    44821 aatcattgca aaccctttca aatacaaatc tcccaatttt taaaatcaac ttacttttct
    44881 ctgctattgg aataaatact gcgggactta taaaaccatg ttctggatga ttccatctta
    44941 ttagtgcttc actccctatc atttctccag ttacaagtga aaacttaggt tgataataca
    45001 cttcaaattg attttttacc aatgctattt ttatatcatc taaaatcatt gattcttcac
    45061 taagcttatt cctaacatct tcattataaa tagcataatt tatattttta cctttagcaa
    45121 cagacctagc catatttgct ttatttacag ccttttgaat atctacttca ccttcttcaa
    45181 caaaataaat acccacacac agagagattt caaagactat actcaaatta ctttcaatat
    45241 tacttcgtat attatcaagt tttcttataa ttttatttct tccattttta taatcacaga
    45301 aaattgcaaa ataatcactt gaaagtcgtg catatacctc accttctgtt aattcttctt
    45361 ttaaagcttt tgctattgta attaataaat tgtcaccaaa ttcatatcca aaagtatcat
    45421 taatcatctt aaagttatta atatcaaaat atactataat ataatttaaa gatggtttat
    45481 ctaaaagata cttactacac tctctataaa atttattttg atttcctata tatgtaactt
    45541 tatcttcata agccaaactt aatatttctt tttcattaga tttttttata taaattatat
    45601 aaaaaataat tgcaataaat attaatacaa tagataacac tgcatataca gctctgttga
    45661 ttatttgttt tgaattacta aatacaacag tttctggaat tgaaaaaatt aaattccagt
    45721 cttttatata attattttca ctattcccat tatccatagt ggcatatcct aaatatcttc
    45781 tttcttccaa catatttaat acagttgcac ctgttttatt ttcttgcaat tcttttttta
    45841 atctatttac ttcttgaata tcattatcct gttctaacaa tttataaata ttttttgata
    45901 ggctatcccc ttgtggatga aggataattg taccatcatt ttttacaaca taagatatac
    45961 tattattttc ataaaaagat atattaataa gtttcatcag agtattgata ttgtaagtac
    46021 aatataaaac tccaactact ttatcattat ttaataatgg tactgaaata gcaatcttct
    46081 ttatagactt atttacttca tcaacatatg gacttgaaac atacttctta cctttaattg
    46141 agtttttgaa aaattctcta tctttcactg aaaacttatc acctttggta ttttcagcat
    46201 taccatttat atcaactatt gccatagtgt aaaatttttg attttccatt tcatctaaaa
    46261 gatttttcat tttttcctca ttaggtatat ctggatttga agcataatag ttagaaaaaa
    46321 tatttaaaac atctaaatct ttttcaattt gtctttgtgc tagttgagag ccttgtactg
    46381 caacttcttt tatatgtatt tttgcctctg acataagaag atgttttgta tcttctatat
    46441 agatataccc cattgtcaaa aataatgatg ttaaaaaaag aattgtaatt aatgtaaaac
    46501 ttttatttct ccctgtcatt cgactcctcc cctaattagt caatacttca tatagataat
    46561 atatcctttt tataccaatt catcatttat attgtatata actattctta aatgttacac
    46621 ataaaatcaa cataataacc ataaaaacat tcttttatta ttataataac actatttaat
    46681 tgaattttaa attaaagaat aaaaactatc cattttctca aacaaaaaaa ctcttataca
    46741 ataaaacttt gttttatgta taagagtttt tattttatct attttatagc aaacttatct
    46801 tccataatat gaatttaaat acatatcttt tatttcattc ataagtggat atcttggatt
    46861 tgctcctgta cattggtcat caaatgcttg aattaccatt tcatctattg aatccataaa
    46921 cttagattct tcaactccaa actcttttat agatttagga ataccaactt taagttttaa
    46981 atcttcaata gctaccaata aactttggaa tttctcttca tcagaatttc cttttaatcc
    47041 taaaaatgaa gctatttcag catatctctt taatgcttct ggatatttat attgagagaa
    47101 tgctcccatt ttctttggtg catctgaaga attaaatttc ataacttctg ttatcaaaag
    47161 agcatttgca acaccatgtg gtacatggtg gaaagcacct aatttatgag ccatagagtg
    47221 acaaacaccc aaaaatgcat ttgcaaaagc catacctgcc attgtagaag catttgccat
    47281 tttttctcta gcttctgggt cttttgcacc attatcatat gcacgtggaa gatattcaaa
    47341 tatactctta atagcttgta aagctaatcc atttgtataa tcggttgcaa gcatagatac
    47401 atatgcttct aatgcatgag ttaaagcatc tataccagaa gcagatgtta agctttttgg
    47461 catattcatc atcatatcag aatcaactat agccatattt ggcatcagtt cataatctgc
    47521 taatggatat tttactcctg tatcttggtc agttataaca gcaaatggtg ttacttctga
    47581 accagtacct gctgatgttg gtatagctac aaaattagct ttttcaccca ttttagggaa
    47641 tgtatatacc ctttttctta tatccataaa tctcatagct aggtcttgga aatctacatc
    47701 tggatgctca tacaatgtcc acattatctt tcctgcatcc attgcacttc ctccacctat
    47761 agatattatt acatctggat taaattctct catagcttta gcgcctatct tagcacattc
    47821 caaagttggg tctggttcta cttcaaagaa tgtagtgtgc ttaatattca tttcatctaa
    47881 taaatcagta actgctttag tatatccatt attataaagg aatgtatctg ttacaataaa
    47941 agctctcttc ttattcatta catctttaag ttcttttaat gctactccta aacaaccttt
    48001 tttgaaataa actttttctg gtgctctaaa ccaaagcata ttttctctcc tctcagctac
    48061 tgttttaata ttaagtaaat gcttaactcc tacattttca gatactgaat ttccacccca
    48121 agacccacat cctaaagtaa gagaaggagc tagtttaaaa ttatataagt cccctatacc
    48181 accttgagaa gaaggagtat taattaaaat tctacaagtt ttcatggctg aagtaaactt
    48241 atcaagtttt tctctttgat ttacatcatc aatatataat gaagatgtat gcccaaatcc
    48301 accatcttct actaattttt ctgcttttct aactgcatca tcaaaatcac ttgccttgta
    48361 cattgcaagt actggtgaaa gtttctcatg agcaaaagct tcttctattt caactgattc
    48421 tacttcacct attaaaacct tagtatctac tgggacttca aagcctgcaa gttttgctat
    48481 agtacaagct gtttgcccaa ctattttaga atttaatcct ccatttacta aaattatatt
    48541 tcttattttt tctgtttcat ctttatctaa taaataagca ccacgctctt taaattcttt
    48601 tttgacttca ttgtatatat tttctaatac tataaccgat tgttcagaag cacatatcat
    48661 accattgtca aaagtcttag atacaagaat tgagtttaca gcagttttta tatctgcact
    48721 atcatcaatt attgctggag tattacctgc accaactcca atagctggtt ttccagatga
    48781 atacgctgat ttaaccattc caggacctcc tgtagcaagg gtcaaatcca cttctttcat
    48841 aacagttgta gttaattcta aagatggctc atctatccat cctattatac cttcaggagc
    48901 tccagctaaa acagcagcct caagaacaac ttttgcagcc tctatagtag aattttttgc
    48961 tcttggatgt ggagagaaaa ttattccatt tctagttttt aaagctaaaa gtgctttaaa
    49021 gatagcagta gaagttggat tagttgtagg tacaactgct gcaataacac ctattggctc
    49081 agcaatttta gtcataccaa atgcttcgtc tttctctata acaccacaag ttttagtttc
    49141 tttatatgca ttatatatat actctgaggc ataatgattc tttataacct tatcttcagc
    49201 aattcccata ccagtttctt tttgtgccat tatagctaga ggaactcttt gcttattagc
    49261 tgccaaagat gctgctaaaa aaattttatc tacctgctct tgactatatg tagcaaatat
    49321 tttttgagct tctctgattt ttgctagttt tttaactaag ctttcaacgt tatttacagt
    49381 atctaaacca actgacataa cttcattttc ttttttattt tctattatta cttgttcttt
    49441 atgagccatt ttaacaacct cctgagatag ttttttattt attatgttaa tttattaaca
    49501 ttatagcatt tactttttct attttcaagt tattttttta acattttatt ttttatctaa
    49561 tataacacca tttctactaa taatatctag ctttacaaat acttaattta actagatatc
    49621 actttatttt ttacgcaaat cactaatgaa atcatctaaa aacttaagga taacgttatt
    49681 tttttaacgt ttatattttt gtatttttta caaataaaac tttataaaaa gaatttttaa
    49741 tatgatactc tatcaagttt aaaaataaaa atacttgaaa atgaaaataa aaacttaatt
    49801 aacaagttaa agtaaatata gttaaatgta caaacattta caacatatag tataattaaa
    49861 aaaatatata ttcatgattt ttatatacta tgaaataaaa tatatttaat attatataga
    49921 taaatttgaa gtttataaga aggaggataa gcatgaaaaa attaaaaaca attagcattt
    49981 tttctttgat tataagtgta attttaacga ttggaggtat tggaatagta acttattatg
    50041 tagataattt gtttattaga ggactatcgg tatttgtttt aattatgtcc tcttcttttg
    50101 tatcaacaac agttagattg atatttgaag agtcaaaacg atataaattc taatttaaaa
    50161 attggattaa gtaaataatt ttaatatacc aaaaattacg gagattagct caaaatagac
    50221 tttttagtcc atgaggcatt tttttgtatg aaattaaata tattttcatc atttcaacaa
    50281 gaaaaagagg cttctctcta ttttgagaca gcctctttgc tagtattgta tctaatacat
    50341 aatattataa gaaccaattc tattgatgtg gactttttat aacaggctgt agctgtttgt
    50401 tctctagagc aagttctata gttggaatta ttagctcagt atgtgcaatc atagaattag
    50461 gctttgcttt gcaattatct tgaccaaaag gtacaaaata tatattttta gaattaagga
    50521 gaatgccaat atttttgaaa ttaaagctta gagcatcatt cgttgatatt gatattacta
    50581 gtggtttatc attacgaaga tgaccttttg ctgccattaa tactggtgaa tctgtaatac
    50641 catttgctaa ttttgcagca gtatttccag tacaaggagc tattattata atatcagcaa
    50701 ttcctttagg tccaaatggc tctgcctctt caatcgtaac tataggttta tttcctgtaa
    50761 gttcatatgc ttttttcata aactcttcag aatttccaaa tctacaatcg atattttgtg
    50821 atacatctga aaatatagtg tgtacattag ctccttcttt aaccaaattc tctaactcta
    50881 taaatatttt atcataagta caaaacgaac cagtaaatcc tactccaatt gtaagtcctt
    50941 caagtctcat ttctaatccc ttctttcatt tataatattt tctatagcgt taacaagaat
    51001 ttcaccagat gtctttggtg aatatttacc aggaagccca aggcaaagag tcgcattaat
    51061 ccctgcttct ttaacaacag aataatctac tcctccagga gaagaagcta tatctataat
    51121 agttacttct tgtgatactt tttctaacct ttctttcgtt aaaatacact ctggtatggt
    51181 gttaaaaata taatcaaatt gtaaaagttt ttcatcaagt tgacttaaag aaatactgga
    51241 atatccaaat gcattagccg tacttcttgc atcattattt cttgcgccaa cacatacatt
    51301 tgcatccaaa gcgtataatt tttttgcaag tatttgtgca catcttccat aacctaatac
    51361 taaacagtta ctttgatgta tattgattat acttctttta atagcttctg ctatagcccc
    51421 ttcagctgta gcaactgcat ttaaaatagc aacatcattc attttcataa aatcataaat
    51481 ctgaatagag tgttgctcgc aaaatttaca aactttagtt ggtatgttac ctccaaataa
    51541 tatttgatta ggagataaat taattagcaa gttatcaata gtagcatcaa gacaatcatc
    51601 cgtattttca atattaactt gattctttgt aaatggaatt ggacataaaa taatggaact
    51661 tttggatagt gcttcagcta gtgaagttga ttttccaacg attccattta taatttcatt
    51721 tgatatccca taagtgacta cagaataccc tttattaatt agttttttta ccatgtacac
    51781 ttgtcgcagg tctcccccaa taacagttat atcgtattga tttgacatat tgtaagcctc
    51841 ctttactata tacaatatgt ttttatcaat acattggttc actaaaatta ctaaatatta
    51901 tacttataac atttttaata atgctaatct atctatcttt tcagaagagg tatatggaaa
    51961 tgaatttaaa aagataattt tctttgggat ttcatttttc attagattat ctttcatttt
    52021 aatcaacaaa tctttcttcg taattttgtc tatagttatt acaaataata caatttgatt
    52081 tcctcttata gtgtctgagt acggcaatac aattgcatct tcaatttgag gaattttttt
    52141 aacttcattt tcaatcttag tagaactaac tttaaatcct ccaatattta taatgtcatc
    52201 acttcttcct tcaaatatta agtatccatc attatcataa tatccaatat cattgacaga
    52261 ataaggtttg gttacattat atactgcata ttctgtattc acaaaaattt ttccttctct
    52321 aatatatata tctattcctg gaaatggtct gcctacactt agaggttttt caattagttc
    52381 atcatagcat aaatatgtaa tataatttaa ttcacttgcc ccatagtata aaataatttc
    52441 agactttggc atatattttt ttagatttct tgcagtatct tcaaataata attgagaccc
    52501 tgtaaaaata cttacaactt tgagtactgg ttcttttaag tgtctaacca aaagttgaag
    52561 ttttgtaggt ataaggtata tgttagttac attatattgt tttattgtcc ttatccatga
    52621 tttacaatta agacctgaac ttattacaat actacctcct tcatataata ctgacattat
    52681 agaatttaga ttccctgtaa aactaagtgt tccattaaca aacaagattg attccccaga
    52741 gatattaaaa atatcatttt gtataggaaa aaatccagcc caactcttat atgttctata
    52801 taataccttt ggaacacttg tagaacctga agataaagct cccatacaaa tacttttatc
    52861 taaataattt ataaggttct tatattgata catatatata ttaaatttag gattaaaaat
    52921 agaaaaatta actttttttg ctacttcatt attgttatca tcatataaat caataacgtc
    52981 catagattca tcagatataa tgattgaaat attatttttt aataaaatat catctaatac
    53041 ttttttagaa agattataat gacaaataat aggaatattt tttgaataat taatagcaaa
    53101 aaaactaatc aactgaaaca ttatattttt agaatatatt aaaatcggga tattttcacc
    53161 taaagaaatt tgctttccta gttcctcact ataactcaat atattttcat aagtatattt
    53221 ttctccatct ataattaaaa aatttttatt tttatgcctc tcttttttta ttcttaaaag
    53281 ttcataatag ttcataatct acaccctttc aagcagtaaa gctgaaccta gaccaccagc
    53341 tgcagctata gagcaaattc catatctacc cttagtcttt tctaatgctt ttaaaagatg
    53401 taatgcaata attgcacctg aagcaccata tggatgtcca tacgctaaag ctccaccaaa
    53461 tatattatat ctatctatta actctggata ctttctttga aacaacacat ctataactgc
    53521 aaatgcttca ttaaattcaa cagcagatac atctaaataa ttaatgcttt ctatctctaa
    53581 aagtttatcc attgcttgta tcgctgatgt tggacttaag gttgcatctg ttcctattgt
    53641 gcatgtatta ataatttttg ctttaggctt cttttttaca tgttctaaat acttcttaga
    53701 acatattata ataaatgaag ctccatcatt aataagacat gaatttgctg catttgtaat
    53761 agtttctttt cctaaaattg atggcatcct atctaataac ctttgactca tcttatcacg
    53821 tataccttca tcacaggtac tattatttat aggagatata atatcctcaa gtattttttc
    53881 ttctcttgct tcttttgctc ttttatgact ttcctttacc caaaaatcca agtctgcttt
    53941 ctcaatttga tataactctg ctactctttc cgccccttcg agcatactat tttggctatt
    54001 atcatcagga gaaaattgag caactgtata atttggatta tttgtattat atcgcttatc
    54061 attcttatga tatgtgcgca ttggttgtag agaactactt tcaaaaccac ctgcaataat
    54121 taaatcacac tgaccagatt taacctttga aaaagctatg tctatactca tcatagcaga
    54181 agcacactgc atatccacag taaaagcagg tacttcattt gacactcctg ctgttaaagt
    54241 cattaatcta gctatattcc ctcctgtccc aactgcattt ccacatataa tctcatcaat
    54301 tttgtcaatt tcatactttt taattaaatc ttttaaaaca cttgctccta gtaattcagg
    54361 ttgaacaaat tgaaaaattc cattttttag acctatatga cttctcaatc ctcctaatat
    54421 aaaaactttc ttcatacatt acacccttcc ttatattata gatttatcat agatttcttc
    54481 tgagtacatt gttcagttta gtacctaaaa aagatgcaat aaccgacttt attgtatctc
    54541 caattaaaaa tggtactact gctgcaaaaa gtgctgcttc tactgtcatt tgattaaaat
    54601 agcacataaa tactgttccc ataaaatata taataggcat tccaacaaaa attgttatta
    54661 gtatatatct cttaatatca ttatttcgtc cttttaataa actaataatt agtgctgcaa
    54721 acaaaaaccc aaaataaaaa ccacctgttg ggcttaacaa ctttccaata ccagatgtac
    54781 ctccaccaaa tactggaatc ccaattaaac ctatcattat atatacacca actgtactaa
    54841 atgcttgtct tggtgttaaa attaaagcta ttaaatttat cattattgtt tgtgctgtta
    54901 ctcctgctgg agtaaatggc aatggtatat atatgtaaga tgatatacac aataatgcta
    54961 cacatattga cattttagtt aaatcttgaa tatttacttt agtatttttc atttttataa
    55021 ttcccttcat atgtcttatt tcatttagtt tatatgtaca tatacaaagc ttctattaaa
    55081 gctttgtttt agtataaaat aaagacgttt ttttgtcaac tattataagt atttaggttt
    55141 acatttaatt tataagtaaa ctttatactg ttactttata ttttaatagt catttataaa
    55201 actttgctta aaaacagctc tatttagact atacttgtaa attataatat gatatatttt
    55261 tatataagat tttattttat ataaaaaata gtaacaaata gtttatacac aagatattta
    55321 aaaggaagtg atgatatgaa agttaatgaa aataatataa ataatataga tgaagagtta
    55381 gatgaactta gtaggaaagt ttacttagag tcaccagatt tttggataag tatttctgac
    55441 aaaataatag ataaagtatt tgatgaaatg ttattatttt ttgcagttaa atataactat
    55501 ttcaatatag tatgctttgc tattgaaaac aattatctgg atttaaacat gccttcaaaa
    55561 aacaaaaaat acactaatat aatggagcat ttgttagata catcaaaaca gaatgaagat
    55621 aaaaaaattt ataattatct tctaagctta aataaaggta ataacctaga tgaaagacca
    55681 attttagatg aattaaactg taatattagt agtgatactt ttgaaaaaaa agatatatat
    55741 cttccaaagt acttatgtcc aagttgtaat tctaatatat ttgatacagg ttataaagta
    55801 tcaaatgaca taacatataa attttcttct aaagaagctg aacctattga aatgtgtagt
    55861 gaagtttctt ctatcagatg ttgtaattgt gatgaattat tagaagatat tacacttgat
    55921 aaactatata acctatgcaa aattcaaaac tgtaataatt gtggttctaa tttaactaag
    55981 gtaggaatta ttgcaaagaa aaaaatgaac tttgatgaga atactgaaaa atttaaagat
    56041 gtctcaacat cttactgttg tggaaattgt gactctgaaa taaacttatc tcaaagagaa
    56101 cattttaaga tttaaatatt tataggctaa agtaataaga ttacattatt agatactgta
    56161 taagtttttt aaatctgtaa aaataaatac acttataaga attaaaattg ctatgaaact
    56221 agttcttgat gaactaattt catggcaatt ttctataatc taataattat taaattttaa
    56281 caaacct
//
LOCUS       DBGJFF010000008        55452 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.008, whole genome shotgun sequence.
ACCESSION   DBGJFF010000008 DBGJFF010000000
VERSION     DBGJFF010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 55452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55452
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.008"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            312..1145
                     /locus_tag="ACOIAF_000578"
     CDS             312..1145
                     /locus_tag="ACOIAF_000578"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HGZ2667605.1"
                     /translation="MRNKKKKINRKKLYLLLSAIVVCLAFIVVGVRVSSLNMKENEIS
                     RNAKLSSSTITDIVSNTSIEVSKGPSKSSGKIAYITIDDGPSKFTDQMIKTLNKHNVK
                     ATFFMIDGNMKEYPQQVKNIIKNGNTAGFHSVSHDIHKLYVTSTSAKEEFDTNDQTFY
                     KITGKHSKVIRIPYGSKPYTPQASYQALVDAGYKIWDWDLDTEDWRSNSSQIVQNVKN
                     HIKNRKGEDKDQLVVLMHEKKQSAEALDSVLKFLSDEGYEFAPVDQNQIPKNYWLRNL
                     E"
     gene            1214..2170
                     /locus_tag="ACOIAF_000579"
     CDS             1214..2170
                     /locus_tag="ACOIAF_000579"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HGZ2667606.1"
                     /translation="MNKKLYIGMVGILSLMMVGCNKSLAKVNDVEITKEQYKKTKAVL
                     SATNNYINGQSLDELEKTLDKKGRNKLENVIISFMVDNELLYQEAKDKGLTPSKSEVD
                     SKYQELEDKMNLNTSYKEKMDKAGVDKDYLKQEISRDLAIDKNKKAFEDRINISDNDM
                     EAYYTSHKKDFNVEEVSASQILISTLDKNKKEVSKDKKEALKKKADNILTKIKNGESF
                     ESLAKKYSDDKATGKNGGQLGYFTKDDKNAEFTKEVFKLKKNEVSNVFETSYGYHIVK
                     VTDKRERQKSFNECQSLIRESILNEKYIEHIKKLNEDAKIDR"
     gene            2222..2704
                     /locus_tag="ACOIAF_000580"
     CDS             2222..2704
                     /locus_tag="ACOIAF_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HGZ2667607.1"
                     /translation="MFKSLKRTKVEKYIIDNKDSFYRIAYSYTKNEEDALDVVQEAMY
                     KALYSVENIKEVNYIKTWFYKILVRTSIDFIRKNRKYNNMTDIDLIDETGEYDKYTDL
                     DLRRALEELPIEYKSIIILRFFEDLKIEEVAIILDENVNTVKTRLYTALKKLKLKIEE
                     "
     gene            2716..3585
                     /locus_tag="ACOIAF_000581"
     CDS             2716..3585
                     /locus_tag="ACOIAF_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HGZ2667608.1"
                     /translation="MTSKERLNKLKDEYHKIPIPKKLDTIINHEKIENIYREKNKKVN
                     RLRFKVAIAFACIFTVLVNISPVFADNFSKIPVIGAIVEVITIKNYSLKSENYEAEID
                     IPKIRGLKDKNLEQRLNSSFMEDGKRLYHQFQERMEKIQSSKNKGYKSLSLSYSVKNN
                     SKKFLSIEMTKNEIEASSYVSKVHYTIDKKRQIVLTLPMLFKDDKYIKVISDNIKEQM
                     REQMKKDSTKSYFIDQKKDLPVEDFKTINKYQDFYFNKNEDLVICFDEYEVAPGYMGA
                     VEFVIPYKVIKDL"
     gene            3628..4383
                     /locus_tag="ACOIAF_000582"
     CDS             3628..4383
                     /locus_tag="ACOIAF_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HGZ2667609.1"
                     /translation="MTTYKKLKKSIFKIAIFSICLLVSLVDTNPIFAGSSFNETSNSD
                     TIKVLNAKSINFKQDNYEVNVSIPKIKGLKDKTLENKLNNEFLQDGKKIYSEFQNKMK
                     KEDNKGHRYLNVIHNVKNNSKDFLSIEMTKEEIEASSSITKKHYTVDKNRRILLNLPI
                     FFKDSRYIRAISDNIKEQMRNQIKKDDTKSYFIDQDKDSPVKDFESINKYQDFYFNKN
                     GNLVISFDEYEVAPGYMGAVEFVIPNKVIKRLR"
     gene            4425..5246
                     /locus_tag="ACOIAF_000583"
     CDS             4425..5246
                     /locus_tag="ACOIAF_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HGZ2667610.1"
                     /translation="MNIYEQAIRLIENNEDFAFATITSHSGSTPRETGAMMIVKNDST
                     IFGSVGGGSVEAECIKHAINVIKNRESMLYKFTLNKSDVAKLGMICGGTGEIQIDFID
                     SKLKSNIEKFNKRLKENTSKAYIFGAGHISRDVAVILSLLEFRTVVIDDREEFANHER
                     FPESEVVVLDSFENIPDFPTDENSYIIILTRGHLYDSSALEWALKREAGYIGMIGSRT
                     KIGLTYEKLMKKGFKKEELSKVHAPIGIKLNAQTPAEIAVCIAAELINCRANKEK"
     gene            5251..6012
                     /locus_tag="ACOIAF_000584"
     CDS             5251..6012
                     /locus_tag="ACOIAF_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HGZ2667611.1"
                     /translation="MNTGSKDIDTLKVNFRGIEIKAVLVYKNRKNISIKIDPFGKITV
                     MSPPKISKKIIKDIIIEKGDWILKKSEKCKGREEVYKQRMFITGEKFLYLGEEYHLVI
                     KELLRDSVKKSNCRITINEYQIVVQTNDTSTDFIKKSLKSWYKMESERIVLERIDFLK
                     LNCEIMKQLVPASVKVKEQNKRWGSCTAQKNIYINSKISMARLDVIDYVIIHEFSHLV
                     HMNHSKKFYDLVKSIMPDYKDKENWLKINGYKIII"
     misc_binding    6173..6430
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6543..6782
                     /locus_tag="ACOIAF_000585"
     CDS             6543..6782
                     /locus_tag="ACOIAF_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HGZ2667612.1"
                     /translation="MENTVYAKLNQGIDSFLRVAMTLRRREVDIQSISMTVDNMNNSG
                     IKLIVNEEKTSLDSVLNHMKKLHDIREITVERMRH"
     gene            7035..8081
                     /gene="ilvC"
                     /locus_tag="ACOIAF_000586"
     CDS             7035..8081
                     /gene="ilvC"
                     /locus_tag="ACOIAF_000586"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HGZ2667613.1"
                     /translation="MSNKNKYHKNKKKYWEEMKMARMYYEKDVDLEVLKNKKVAVLGY
                     GSQGHAHAQNLRDNGVHVMIGLYDGSKSAQKAKEDGFEVKSVAEATKESDLTMMLMPD
                     EKQKKVYEESVKDNLKEGQTLAFAHGFNIHYNQVQPPEFVDVVMVAPKGPGHLVRNVF
                     TKGSGVPALFAVYQDHTKKATETVLAYAKGIGATRAGVLETTFKEETETDLFGEQSVL
                     CGGISELIKLGYKTLVDAGYQKEVAYFECLHEMKLIVDLIYEGGFERMRYSISDTAEY
                     GDYVSGKRVITDAAKQGMQNVLEDIQNGKFAKAWIKENEEGRENFLKTREEEYNTEIA
                     EVGRNLRSMMSFLK"
     gene            8134..9822
                     /gene="ilvB"
                     /locus_tag="ACOIAF_000587"
     CDS             8134..9822
                     /gene="ilvB"
                     /locus_tag="ACOIAF_000587"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HGZ2667614.1"
                     /translation="MNGAKVILECLKKEGIDTIFGYPGGAVIPLYDALYDYSDDFKHI
                     RTSHEQGLVHAADGYARSTNTVGVCFTTSGPGATNAITGIATAFMDSSPMVVISGQVP
                     TSLLGKDSFQEIDITGATLSMTKHNYLVRNTKELVPTIKEAFRVANSGRKGPVLVDVP
                     KDLFLAEMDFSGEDYDLCQIDDYMDYKSDFDLDDETNIKLLNEAIDIIKESKKPVIYA
                     GGGVKSSDSEEILEKFATKIDTPVLNTLMGLGNIDRKNELSLGMVGMHGSRESNLALS
                     NSDLVIAIGARFSDRVISKSSEFAKNAKIIHIDIDPSEISKNIESNVSLVGDVKLVLS
                     LLIERVESKNNSNWKEEIKRFRKSEGVQTYEFHPQNILKKINEKYETLKKPTVVVTDV
                     GQHQMWAAKYWNFKGNKSFITSAGLGTMGFGLGAAIGTKVGNVDKNVVLVTGDGSFRM
                     NCNELATVANYNVPMLILLLNNRTLGMVRQWQKLFSNQRYSQTDINENVDYVKLVNAY
                     NIDGYKVSSMEELGKALDMIDFNKPVFLQCDIDKDYDVYPIVAPNDALENLICN"
     gene            10033..10710
                     /locus_tag="ACOIAF_000588"
     CDS             10033..10710
                     /locus_tag="ACOIAF_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HGZ2667615.1"
                     /translation="MFKHDKALLKEVKVERPNPQYAVLMQEQLGGANGELKAAMQYLS
                     QSFRIKDPQIKDLFLDIAAEELSHMEMVAQTINLLNGHDVDYNSVNTGEIETHVLTGL
                     SPVLINSSGAPWTANYVTVTGDLVADLLSNIASEQRAKVVYEYLYRQIDDKYVKETID
                     FLLNREEAHNALFRDALNKVKDTGSNRDFGVTEDSKLYFDLSTPGPNHDTKIDINPPS
                     FEKPLKK"
     gene            10884..12920
                     /locus_tag="ACOIAF_000589"
     CDS             10884..12920
                     /locus_tag="ACOIAF_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HGZ2667616.1"
                     /translation="MDSNRKPNNLINEKSPYLLQHAYNPINWYSWNDEAFKKAKEEDK
                     PIFLSVGYSTCHWCHVMEKESFEDEEVAEIMNRNFVAIKVDKEERPDVDSVYMTVCQA
                     MTGSGGWPMTIIMTPDKKPFFAGTYFPKYSRYNRPGVIDLLENVSEKWNTSRDILIKS
                     GDEIIEALKDDFGVKNTEGDLSKEMLSSSVRVFKAIYDENYGGFGNAPKFPSPQNLMF
                     LMKYYSIEKDKDVLKMVEKTLDGMYRGGLFDHIGFGFSRYSTDKKWLAPHFEKMLYDN
                     AMLTIAFLDAYKITKKELYKEIAIKTIDYVVREMKDKEGGFYSAQDADSEGEEGKFYT
                     FNPLEIIEVLGEEDGIFFNNYFDITSSGNFEGKSIPNLIKNKEYERHNEKIADLSKKV
                     FEYRKERTSLHKDDKILTSWNALMIVALTKAYSTLKNDIYLEYSNKCLNFINNNLVNE
                     SGRLLARYRDGSSDYLAYLDDYAFLIWAYIELYESTFNMKYLEKALNLNESCINLFWD
                     YEKSGFYIYGKDSENLIARPKDLYDGAIPSGNSVQLYNLIRLAKITGDNRLEEMSYKQ
                     LKLYVDNVKSSPTGYSFYMLSLMFELYSTKEIICIFKEDSDLIAFKELISENFIPNAT
                     FLAKKYNEENTIIGFLNNYILKDDKTSYYVCQSNSCSQPINDLQKLKDMILGVE"
     gene            complement(13111..13677)
                     /locus_tag="ACOIAF_000590"
     CDS             complement(13111..13677)
                     /locus_tag="ACOIAF_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HGZ2667617.1"
                     /translation="MDYSNELKELFLMNQTYATLFTLTNKIQIEGDKYFGILTSRQYM
                     TILSILHLPEEETTLNNIARKMGTSKQNINRLVANLEKNGYVDVIPSPHDKRAINVKV
                     TDLGKKVMVTCSRTGINFMADVFHEFTKDELETLWSLLKKMYRFNGEEQDGFEEDANF
                     MEYEEIDKIKSEALEEFAKRRKRVNKND"
     gene            13965..14900
                     /locus_tag="ACOIAF_000591"
     CDS             13965..14900
                     /locus_tag="ACOIAF_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HGZ2667618.1"
                     /translation="MKCTNFTFKGEEGLDIYTYKWEDENIKKPKAVIQIAHGMAETAQ
                     RYETFAKVLTKNGYIVYINDHRGHGKTAKIIENVGHLAEKEGFRCLVEDMYTLTNIIK
                     KENEDLPIYLFGHSMGSFASQRYIMDYSNNLSGLILCGSNGKQGIILNLAHLIINREI
                     KKYGRRSKSNKINNLIFGGEIIRRNEKTKFDWLSRDKEQVEKYINDPFCGVVCSCGFF
                     YDLVQGLKEIEDKENLKKVPLDIPIYIISGDKDPIGKNGKGVLRLRDRYIKLGVKDVT
                     CKLYKDGRHELLNEINREEVFEDIICWLNNKIEIL"
     gene            15373..16587
                     /locus_tag="ACOIAF_000592"
     CDS             15373..16587
                     /locus_tag="ACOIAF_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGZ2667619.1"
                     /translation="MQIKIGKVIQRLRKERNLTQEQLAKFIGVSTPAVSKWESGNSYP
                     DIELLPLLADFFNVSIDKLLNYKIDLSEEEVMKIYKELESGFARIEIDLSTEEPKEEF
                     RQDLESVKKLSNMYIEKYPKSYLLKLRICSLYQMYSYKFGKSELNDRVKETTNILEDI
                     VRNTDDIQIKETALIILSNAYCMLEDYEKAELYLNMIHKSIGDTSVNLAMIYLKQNRL
                     EEAEILLQNKLFSNVFNISMDCKGIINVYKNQYKELKKKLENRNFNKNAIEREMEYIK
                     NKLLGYANLSLEIKKMLSEDKGAFFSMYMDYMELSLIFLFFNMKEEAKKALYSLKEIL
                     EKYPIHESLDVSQMRFFDKVESKNLYTFNIYTNLLIVLNDDSYNELREEPIFKDVIEK
                     ILDMEKMLKNKE"
     gene            complement(16936..17457)
                     /locus_tag="ACOIAF_000593"
     CDS             complement(16936..17457)
                     /locus_tag="ACOIAF_000593"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGZ2667620.1"
                     /translation="MKFERLRTSENKLYYKAMELYKISFPFHEQRKSSLQAEILKNKE
                     YQFNLIYDKNQFVGIILCWETDDFIYVEHFCIFPEMRNNRYGQRALELLNKKGKTIIL
                     EIDLPIDEISIYRKAFYERVKYKTNNYEHIHPPYHEKFNGHNLIVMSYPEKLSKMEYD
                     KFNHYLRNNVMSF"
     gene            17813..18610
                     /locus_tag="ACOIAF_000594"
     CDS             17813..18610
                     /locus_tag="ACOIAF_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HGZ2667621.1"
                     /translation="MEKREKRIIISSLLSVSILMGLASIYSILNKEDISLTVKGQEQK
                     VSSFKKTVEELLDEQGVKYNSEDKINPSLDTELKDDMKIKVVKVTKSKKEEIKKIPFD
                     TKHVNDSNLLKGKSKVYQEGQEGEKKLVYNLTYHDGKLVKKVLSKEVISKEPTTKIIK
                     YGTKEKVLIASRGANIRGGKHMKVVATAYAGDTITSTGTTPRWGVIAVDPRVIPYGTK
                     VYIPKLGMTFVAEDCGGAIKGNRIDIFMNSEGKASNWGRKSIDIYLH"
     gene            18720..19310
                     /locus_tag="ACOIAF_000595"
     CDS             18720..19310
                     /locus_tag="ACOIAF_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HGZ2667622.1"
                     /translation="MKIVCLGDSLTYGFGVSRSNSWTNIVNKETRLEIVNKGINGDTT
                     SGMLARFNEDVVKNSPDIVFIMGGTNDFIAGAGNEIINSNIMAMVHQSYSKNIIPIIG
                     IPLKPDIPNVRKDWSCFTDFNIVSQNLESYSYWIKKFCMTFNTNFVDFYSEYNKNMEL
                     EGYKKLYFDGLHPTKEGHRIMADIFINSINKYIEEL"
     gene            19376..21073
                     /locus_tag="ACOIAF_000596"
     CDS             19376..21073
                     /locus_tag="ACOIAF_000596"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HGZ2667623.1"
                     /translation="MDYTKTYEEWIKGSYFDEDTKLELENIKNNEKEIEDRFYKDLEF
                     GTAGLRGIIEAGTNRINKYTVRRATFGLANYILENTTKEETSRGVVIAHDNRHKSRQF
                     CIESANTLAACGIKAYIFDSLRTTPELSFAVRSLNAIAGIVITASHNPPEYNGYKVYW
                     EDGAQVMPEIANAITEKVNSIHDYSTIPTLTEANKNLVVLLDESQDTKFIEAVKSQII
                     RKDLVKNVGKSFKIVYTPLCGTGNVPIRRALKEVGFENIIVVPEEENPDPNFAGLDYP
                     NPEEKKALNRGILLAKEKGADLVIATDPDCDRVGVAVKTTTGEYALLTGNQIGGMLTH
                     YIIEGLKENNKLKENPTIIKTIVTSEFGADIAKANNVDVLNVLTGFKFIGEKIKLFEQ
                     NKNRSYVFGYEESYGYLVGTHARDKDGVVSSLLISEMAAFYYSKGMSLYDGLIELYKK
                     YGFFKEQTISLTLKGIEGVEKIKEIISYFRENQIDYINSIKVVDKKDYKNGIDNLPKS
                     DVLKYFLEDESWVAIRPSGTEPKLKFYIAVKGASDIEADKKIQGLKKYIDDMVEKLK"
     gene            21287..22126
                     /locus_tag="ACOIAF_000597"
     CDS             21287..22126
                     /locus_tag="ACOIAF_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HGZ2667624.1"
                     /translation="MFGIDKKLIEYIIAKSNYSELSSKSAEVSFFLMLSIFPFLIFTI
                     SSIAYIPILHLNKYIALFRNMMPEGAFAVLSSIIVSAIDNRNLKFLAVSFVLTMWTFS
                     RAVKALIKGMNRAYKVKETRSFFKILSISFLFTIMLLVLIFLSMIFLVYGEKIGYFIF
                     NLVGLDEIFIKIWDILRYTVGIITIIVIFTLLYKYTPNKKLTIKESAPGAIFATFAWF
                     LVSFLYSYYTNYYANYEVIYGSIAEIIVLMTWMYFSSWSIVIGYEVNSRLYFRKIRHE
                     MLK"
     gene            22643..24499
                     /locus_tag="ACOIAF_000598"
     CDS             22643..24499
                     /locus_tag="ACOIAF_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGZ2667625.1"
                     /translation="MDTHNKYVNFIKNIPVPFLYCRIVKRQEDIEYRVEYISKGMGKV
                     LQLEEGICDKNILDVLPVFKSKKYFKELFSNEVDCIKRYIPTLKNWINIKKQIIGDSY
                     IILYFGKIVFDYRQIIDSFDKKEKVAYIKDEEGIYIDCSENLIPILNNNIKTTKDIFG
                     KNDIEVWGENTGKLFRDDYREGVSSKKRFLQNLFEYEETFFMVEKYFLYDEDELLGTI
                     GIVDNIIYSGYSNRNYNSKDLMKMIEHSIPENMFYKDVYGNYIGFNSGFLNLACMNKE
                     ELLGKNSYKISEEEALIDKIFESDKGVVENKKVVTFELNISMNDENKCIEITKRPFFD
                     SYGSVIGIIGTARDISRRKRLEEEMDKTRMEFFANLSHELRTPINLISSSLQVIEKKE
                     ADLIESNDTLKRNLGIIKQNGNRILRLVNNVIDFTKMQSGYLDFKPEESDIIAFIEEI
                     CMSVADFASQNNIQLTFDTEIEEFSMLFDSEKLERIILNLLSNGIKYNKKDGKINIFL
                     YVKDNVFNMKISDSGIGIPKEKIDKIFNRFEQIDNELSYRVKGSGIGLSLVKSLVELH
                     EGSISLKSQLGIGSEFIVSLPVRSKNNIEKYNHKREISNELSKKLEIEFSDL"
     misc_binding    24582..24822
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    24888..25123
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            25208..26425
                     /locus_tag="ACOIAF_000599"
     CDS             25208..26425
                     /locus_tag="ACOIAF_000599"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HGZ2667626.1"
                     /translation="MENKVKIGVLGYGVVGSGLIDIIDNNKEKRNIEIVGILVNNLEK
                     HKDKKYSNIITNNIDDIFNKDIDILVEVMGGLEPSLSYIKKALNNKIHVVTANKDLLA
                     ECGDELAKLASENKVSIKFEASVAGGIPVLKPIIESLEGNNIDSINAILNGTTNFILS
                     KMYDENLSYDMALRQAQELGFAEANPESDVLGYDAARKLSILSTLAYDNRVYWKDVYL
                     EGITDIDEKDIEYAKKLNCKIKLIGQSKYENDKVSAFVRPVLVEKDNILARIDNEFNA
                     VIVNGDSVGEVSFVGKGAGSLATGSAVYSDVIDIIDNRVSSIDSFTKDKIQVNKIVRE
                     KCGALLRFKKCNKDEILNIVENCLVKFDILNDDDELAIMVYADSEYEINNSLCLIKDK
                     GYCEKMNKMLKIS"
     gene            26542..27024
                     /gene="cdeM"
                     /locus_tag="ACOIAF_000600"
     CDS             26542..27024
                     /gene="cdeM"
                     /locus_tag="ACOIAF_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeM"
                     /protein_id="HGZ2667627.1"
                     /translation="MENKKCYSEDWYERGESTAKWFQNDREEYEREAYDEDRERRGSN
                     CGCSDSGENRPRNCERFRREAEIREREAREAFCESSEKKKEALAYECEARKLWEEAEK
                     YWDEYSKYNYKGIEYLAEAARLFDEGMECEARRNGNNGGNNNNCCHKCHKCNCNCCRK
                     "
     gene            27289..28554
                     /gene="hisD"
                     /locus_tag="ACOIAF_000601"
     CDS             27289..28554
                     /gene="hisD"
                     /locus_tag="ACOIAF_000601"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HGZ2667628.1"
                     /translation="MKSIKTSSMKEKVDEKILREQVSSIIEDIRNNKDVALKKYNEKF
                     DRNTRDEFRITKEEIKEAYKHVDDEFINNLKIAAKNIREFAKAQKLSFENPFEKEIYP
                     GVILGQTNIPIESCLAYVPGGQYPLFSTALMLIIPAKVAGVKRIVACSPTMKNTEKIN
                     PKTLVAMDIAGADEIYATGGVQAIAAFTYGTEKINPVDIIVGPGNKFVTEAKRQCYGQ
                     VGIDFVAGPSEVLIIADETSNPVYIAADLLAQCEHDLNARGILLTNSLEVAQKVEKNI
                     ESMLKDLPTKDIAYSSWENNGEIILVDDMEEAIKISNFYAPEHLEVAVNEYNDICDRL
                     TNYGSLFIGNLSAEVFGDYVSGTNHTLPTLKASRYTGGVWVGTFIKTCTKQIFNEKAI
                     QSLAPVAEKLAKEEGLYAHAKAAEVRFKK"
     gene            complement(28757..29509)
                     /locus_tag="ACOIAF_000602"
     CDS             complement(28757..29509)
                     /locus_tag="ACOIAF_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGZ2667629.1"
                     /translation="MSYFQYQGNSCFYKEYGQGKPIIFLHGNTGSSNMFKALVPLYVE
                     NFRCILIDFLGNGQSDRVSQFSPDIWHDEALQTIALIEHLNCGKVGLIGTSGGAWAAV
                     NTGLERPDLVEAIIADSFDGRTLNDNFIDNLITGREKSKQDIQARKFYEWCHGKDWEN
                     VVNLDTKALLQCANEKRPLFHKELCKLEMPILFTGSKEDEMCRHNLEEEYKQMATFIS
                     KASIYIFSQGGHPAILTNADEFAQLAKVFFKY"
     gene            complement(29627..29896)
                     /locus_tag="ACOIAF_000603"
     CDS             complement(29627..29896)
                     /locus_tag="ACOIAF_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3324 family protein"
                     /protein_id="HGZ2667630.1"
                     /translation="MSYQNSIDLLPKELIEQVQEYIDGKVIYIPKKQEHKKHWGENTN
                     TKQVLASRNSQICINFQNGMSIKQLSEKYFLTEKSIQRILKQQNL"
     gene            30371..31465
                     /locus_tag="ACOIAF_000604"
     CDS             30371..31465
                     /locus_tag="ACOIAF_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HGZ2667631.1"
                     /translation="MKNIVGKPQTIKKVNEDLIKNIIKNEGPITKPEISNITELSLAT
                     VNKIVEQLALKNEVKVSGLSESTGGRRAQLFEINANLEHIIALYYYRNCCIGVVANLL
                     GEIIYQEEFNIRMDTYENVNQDTFFVIDTLIKKEKGNNIRAIGIGVPGVVKKGVVKNI
                     PTIKSWEGINLENLIKSKYNTKVFIENDTNITTIGIYQKQYKDKYKNMALMYLEDGIG
                     SGIVINGELYLGSTNFAGELSYLNIEFGKYNSNNLNLEDYIMSLRKRYLESKDNKFKK
                     EILSIISNFIRTLVCVLNPQAMIIQCSILTKSDMEFIEKDVSNNVGEENFPKLIKVDS
                     LREGSINGVISMCLKETDYQYSLSNKKGGY"
     gene            31467..32417
                     /locus_tag="ACOIAF_000605"
     CDS             31467..32417
                     /locus_tag="ACOIAF_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HGZ2667632.1"
                     /translation="MKYLVSIDGGGTKTKFCVSDLDGNILKEHTTGSTNYKSVGIKKT
                     YENINNGFKKILKDLYIDYDDIEYTVFGISGCDSPNDYKIIMDEILKIGINKEKIYLA
                     NDAVLAFYAQADSPGLVIVAGTGSIILGIKEDGEIYRVGGWGYNFSDLGSGYDIGRKL
                     LKKVLLYCDECHEYSDLFSCVLDFFGANSFEQLAYMITDINDNVEIANLASLVIDCAE
                     QGDKLAIEILRESSTELSKLAQVILRKISNPMKMDKSEINIVLSGGTLNKTVYAQMLM
                     NNLKTENTDKNLKFISQENQPVYGGIRLAMALANRRVKHG"
     gene            32410..33594
                     /locus_tag="ACOIAF_000606"
     CDS             32410..33594
                     /locus_tag="ACOIAF_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667633.1"
                     /translation="MDRKKVIVQIFTGGFKAKEIKFNDIKCKLLELLNCIDIEKVIMG
                     WSVDEELYVETKKLLNKYNVELYLWLPCFSEIGLLEKSNLLIDYMGKEVKGYALQEDE
                     NFEFYCPNNKENIKNIKKIYSTYFSDIKFDGIFLDKIRYGSFSNKLNGVFNCFCKDCL
                     NIYEKNNIDKESLMREMNNVSKGVIDNEDTPFGITEYINGKYKFKNKIWEDFFETKNQ
                     NIYKALLEISNYFKSKGLKIGIDTFSPFISYFTGQDLEKLQEVADFIKPMMYRTTKAP
                     AGLPFEFERFIDESIHKNKEIGKRNFLDILGIKEYEGDVYPIEFVLEEIDFMTRNLNK
                     DIYVGVEINKKEIISPVTPKYISENISNINKTTVKGYVLSWDLLSAPKENIDEIIKWF
                     RN"
     gene            33607..35103
                     /locus_tag="ACOIAF_000607"
     CDS             33607..35103
                     /locus_tag="ACOIAF_000607"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HGZ2667634.1"
                     /translation="MSKILEMQQIYKSFGKVDVLKGINFELEKGEVRALLGANGAGKS
                     TLIKILGGVHSQTKGKVILTGNEIMFKNPHHSKENGISIIYQELSLIPTLTVLENIFL
                     GREILKGSVFLNKKDMLEEYMKICEDFDFNIDPHMVVSNLSVANQQMVEIMKAVSCNS
                     EIIIMDEPTTSLTNNEKDGLFKIISRLKSKGKTIVYISHILEEVFKVCDSTSIMKNGI
                     MIGTYDVEDLTKLKITQLMTGNDNHKLIEKKDYTFAKYEEPPVLEVKNISWSNKIKNV
                     SFKVYRGEVVGLAGLVGSRRTEIINLIFGIDNKDSGDIFINGKKVDITSPKVAIKNKI
                     GLIPEDRKNLGLILEQAIYTNSSSVQIDKFKKFLFIDRNKEIEFSKKGVKELGIKVSD
                     VKQEVKELSGGNQQKVVVSKWLEKDLDLIIYDEPTKGIDIAAKEDIFNTIKEFSSKGI
                     GVIFISSDLEEVIRVSDRILVTREGSIVDDLKNENIQVQDIMNSIFNV"
     gene            35126..36076
                     /locus_tag="ACOIAF_000608"
     CDS             35126..36076
                     /locus_tag="ACOIAF_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2667635.1"
                     /translation="MKKAFEKLNLRDYGVLLGFLALCIAISIATPSFLGKQNILNLLR
                     QSSIIGIISAGMTFVIISGNFDISVGAVAALAGAITMKFATGGTNLFASMFLGVAICA
                     VIGLINGIVVAKINVPSLIATMAMVTIVRGLLLMLTGGYPITENIPILDYIGNGYLFN
                     IPIPVVIFFIVVLVSFIVLNKTKFGRYVYSVGGNQEASKLNGINVDSHKVKVFIINAV
                     LAGIAGIVLTGRLGTATAIAGEGYDMDAIASVVIGGTSVAGGSGSVLKTVIGVLLMSV
                     INNSFNLLGIDVYFQYIFKGLIILAAVGFDSYSKKKLASR"
     gene            36097..37059
                     /locus_tag="ACOIAF_000609"
     CDS             36097..37059
                     /locus_tag="ACOIAF_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HGZ2667636.1"
                     /translation="MKKLMKHLALLLSLVMIFGLVGCSNGGDKEKNGEKKIAVLLPGS
                     TGYFVATKQGIDAKSKELGVSVEYADAQWDASKQLSQAEDFMAKGVDMIALCGVDSAV
                     SERIVKAANDSDVPIVAFTNSIGSNPTGEFKGLVTYIGQNEEETGALTGKIAKNLLGE
                     TGGKAVLIEGVPGTTPQVLRKKGLEKELKDSNIEIVYNQTSNWEKEQALKVTEDLIQK
                     KTDFNVIICQDDNSATGAGQALKDAGLKDKVKVIGLGGSKDGLKAITDGLIDGTTYMS
                     AVEEGGLVIEKASKFLKGEKIEPVTQIKQVEVNKDNISEFKGEW"
     gene            37431..38063
                     /locus_tag="ACOIAF_000610"
     CDS             37431..38063
                     /locus_tag="ACOIAF_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HGZ2667637.1"
                     /translation="MARRLFLERIGLIFLGSAILAFGVYNFYYLNNITEGGVLGILLL
                     LKNLFNIQPAIANVVIDGLLLLVGYKFFGKKFLIYSIVASITFSVLYDLFEAIGPLVP
                     QSQNMLLSTILAGVTVGTGVGIVVKAGCASGGDDALALVISKTTSLNIGQVYLATDVI
                     VLLLSLFYLSAFDIFYSLIAVTISGKVIDFIYYHGKSLDMDISNDIVPEC"
     gene            38489..38566
                     /gene="kdpF"
                     /locus_tag="ACOIAF_000611"
     CDS             38489..38566
                     /gene="kdpF"
                     /locus_tag="ACOIAF_000611"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0043462 - regulation of ATP-dependent
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HGZ2667638.1"
                     /translation="MWFLAVVIIMLIIYLVYALINPEKF"
     gene            38613..40289
                     /gene="kdpA"
                     /locus_tag="ACOIAF_000612"
     CDS             38613..40289
                     /gene="kdpA"
                     /locus_tag="ACOIAF_000612"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HGZ2667639.1"
                     /translation="MVQIVIVLAIFMILVIPMGKYLYHIATNQKTFGDRLFDKVDNFI
                     YKVCSIDKKKEMNWKQYALALLFTNAVMVFIGYIILRTQSMHIFNPSGIKSMEQGLSF
                     NTIISFMTNTNLQHYSGESGLSYFSQMTVIIYMMFTSAATGYAAAMAFVRGLVGKKKT
                     LGNFYVDLIRITTRVLLPGALIIGLILVTQGVPQTFAGTETVTTIEGKLQDIARGPVA
                     ALESIKHLGTNGGGFFGSNSSHPFENPTIISNIVEILSMMILPGACVVAFGHMIKNKK
                     QGWVVFGAMSIIFLIGLVVCFKAESAGNPILSQLGLNQSMGSMEGKEVRFGIAQSSLF
                     TTVTTSFTTGTVNNMHDTLTPLGGLVPLLNMMLNVVFGGKGVGLMNMLMYAIIAVFLC
                     GLMVGRTPEFLTKKIEGKEMKLIALLIILHPLLILMFSGLSVAIPAGLEGISNPGFHG
                     LSQVLYEFASSAANNGSGFEGLGDNTMFWNITTGIVMFFGRYVSIIVLLAISSLLASK
                     KAVNESIGTLRTDNFTFTIVLVLVVLIVGALTFFPALALGPISEHLVLWH"
     gene            40314..42371
                     /gene="kdpB"
                     /locus_tag="ACOIAF_000613"
     CDS             40314..42371
                     /gene="kdpB"
                     /locus_tag="ACOIAF_000613"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HGZ2667640.1"
                     /translation="METKKTKFITKDIFKTSIIEAFKKLNPKYMMKNPVMFVVEIGFI
                     VTLILTIVPSLFGDEGNNLRAYNGIVTIILFITVLFANFAEAVAEGRGKAQADTLKKT
                     RKDTVARVIDTLGNEKMISASELKLGDIVLVNAGEVIPNDGEVIEGIASVDESAITGE
                     SAPVMRESGGDFASVTGGTTVVSDWLKIRITTKPGESFLDKMIALVEGASRQKTPNEI
                     ALNTLLVGLTLIFMVVLISLYPMANYAGVKIPISTMIALLVCLIPTTIGGLLSAIGIA
                     GMDRVTRFNVIAMSGKAVEACGDVDTMILDKTGTITYGNRLASEFIAVGNADSKDLIN
                     YSVMCSLKDDTPEGKSIVDLGMKLGSTGKTKEAEEAEFVEFSAQTRMSGINLADGRAI
                     RKGAYDSIIKRVKESGGTVPEDLEENVNRVAKLGGTPLVVCVDNEIYGIIYLKDTVKS
                     GLVERFARLREIGIKTIMCTGDNPLTAATIAREAGVDGFIAECKPEDKIDAIKKEQLE
                     GKIVAMTGDGTNDAPALAQADVGLAMNSGTTSAKEAANMVDLDSDPTKILEVVEIGKQ
                     LLITRGALTTFSIANDVAKYFAIIPAIFTLAIPQMEILNVMKLSTSFSAILSALIFNA
                     IIIPCLIPLAMKGVKYRPMKSQAMLMRNMLIFGLGGVIVPFLGIKLIDLLITPLVMVL
                     NLG"
     gene            42390..43016
                     /locus_tag="ACOIAF_000614"
     CDS             42390..43016
                     /locus_tag="ACOIAF_000614"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HGZ2667641.1"
                     /translation="MKTLKPALLVSIVLLVVCGLVYPLVLTGVSQVAFKDKANGSMIE
                     VNGVKVGSELIGQSFTDARFFKGRVSSVNYNTYTKEDLVPDKDGNTSYGGVSSGSFNY
                     GATNPELHDRVQKDIEKFLKDNPTVKREDIPTDLLTASGSGLDPNISPASAKIQIPAI
                     AKASGISESKLQKIVDDNTSKKLFGVLGEDRVNVLKVNVEVAKILGLI"
     gene            43253..44161
                     /gene="cysK"
                     /locus_tag="ACOIAF_000615"
     CDS             43253..44161
                     /gene="cysK"
                     /locus_tag="ACOIAF_000615"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HGZ2667642.1"
                     /translation="MLYNNALELIGNTPVVRLNNLGCTNGCSNIYVKLEKVNPAGSIK
                     DRAVYEMLEGLEQRGELKKGDVLVEATSGNTGIALSMIGKLKGYEVVIVMPETMSVER
                     RDLMKAYGANLVLTDGKAGMAGSIEKANELLKENPNYKSLKQFENKDNPNAHYKTTAV
                     EIMNDVKDLDIFVCGVGTGGTLIGTARYLKEQNPNIRVIALEPKKSPAISENKTGPHK
                     IQGIGANFVPENYDSSVVDEVILVDDEDAFDTVKLLAAKEGILVGISSGANIFGAIEM
                     AKKYPDKKIVTVAPDGVDKYMSMGIF"
     gene            44188..44778
                     /gene="epsC"
                     /locus_tag="ACOIAF_000616"
     CDS             44188..44778
                     /gene="epsC"
                     /locus_tag="ACOIAF_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HGZ2667643.1"
                     /translation="MFKKINKDIEYIMKNDPAARSKIEVFLLYPSVHAMIMHRMAHAL
                     YKKKKLFTARLISQISRFMTGIEIHPGAKMGEGILIDHGMGVVIGETAEVGNRVTIYQ
                     GATLGATGKDTGKRHPTVGDDVLIGAGTKILGPLNIGSNSKIGANSVVVKDVPNGATV
                     VGIPAKIVKIRNLEPVKKNKKEVSYEYDELDNVYYI"
     gene            44938..45126
                     /locus_tag="ACOIAF_000617"
     CDS             44938..45126
                     /locus_tag="ACOIAF_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HGZ2667644.1"
                     /translation="MKANVNQDTCIGCGLCPSICPEVFDMKDDGKSHVIVDEVPSDAE
                     ESAAEARESCPVDAIDVH"
     gene            complement(45180..46049)
                     /locus_tag="ACOIAF_000618"
     CDS             complement(45180..46049)
                     /locus_tag="ACOIAF_000618"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HGZ2667645.1"
                     /translation="MKLNRNDSCWCGSTKKYKKCHMDMDNKIDSFAKLGHIVPTHDII
                     KNESQIEEIKKSAVINTKILDYISDKIEIGMSTEDINKLVHDFTLQNGAIPAPLDYNG
                     FPKSVCTSVNNEVCHGIPSKDIILKDGDIINVDVSTIFNGYYSDASRMFMIGNVNENA
                     KKLVQVTKECVQKGLEVAKPWGFLGDIGDAINSYAKKNGYSVVREIGGHGVGLSFHED
                     PFVSYVSKKGTEMLLVPGMIFTIEPMVNEGTHEIFVDEENNWTIYTKDGKLSAQWECM
                     VLVTETGVEILTE"
     gene            complement(46246..47490)
                     /locus_tag="ACOIAF_000619"
     CDS             complement(46246..47490)
                     /locus_tag="ACOIAF_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667646.1"
                     /translation="MENNLNTAKRNNTELLQNDSLKVNLQIGVDFTLLSKSLKAFYKK
                     EKDFYCIALAPCNVSSEKANRKISIYDMIKEINILIESITDKNELLSEELFTNNLSEF
                     YDESLGEIYIDLKQAYLYIKKNTDSLNIEDSIEYVFDVDISNDIKAKENPLINFNSLS
                     LVVWSGETKHILQQMNILDLEDIHSYSIENNTNITIYENNTLQYKFDEESENIKDSSI
                     DIIEQMLILPDDLNYDKDEVENMKSRLAKINIKYLQTLKEKDIKIKLINSNLTDEPEF
                     SDLKYQLPPCWVRSGKTWKDVPGIYRNNSIVAKIGYSNPSYANVHSSKNLELHETAHA
                     IDKNVLNKKSNSEEFMEVFAQERYKLYDPKQVAHAYISKFIEEFFAESFVHYYLDEDS
                     KNTLKENCPLTYDFLEKLELNY"
     gene            47781..48092
                     /locus_tag="ACOIAF_000620"
     CDS             47781..48092
                     /locus_tag="ACOIAF_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HGZ2667647.1"
                     /translation="MYFTYIIRCKDDSLYTGYTSNIVRRMNEHKLGINSKYTRAKGFE
                     KLEVYFVTNTKSNAMKLEYYIKKLTRNKKLSIIKNPSILINLIDNKEDYIIGKKIEQL
                     T"
     regulatory      48185..48277
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            48377..49171
                     /gene="thiD"
                     /locus_tag="ACOIAF_000621"
     CDS             48377..49171
                     /gene="thiD"
                     /locus_tag="ACOIAF_000621"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HGZ2667648.1"
                     /translation="MSNYKIPTLTIAGSDSSGGAGIQADLKTFSAIGTYGMSVITAIT
                     AQNTKGVFAVEDLNKKIIKKQIEAVFEDIPPRAVKIGMVSSPEIILEIVENLKKYNPK
                     YLVVDPVMISKSGYYLLKPEAKENLIKYLIPLAYIITPNIPEAEEITGIKIHNVDDMK
                     RVGEEILQLGPKFVLMKGGHLDGEAVDILVGKNIFKVYKSERIDKKNTHGTGCTLSSA
                     ITSYLALGYEITEAVNLSKIYITEAIKRSFDIGHGVGPVHHFYKFE"
     gene            49195..49983
                     /gene="thiM"
                     /locus_tag="ACOIAF_000622"
     CDS             49195..49983
                     /gene="thiM"
                     /locus_tag="ACOIAF_000622"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HGZ2667649.1"
                     /translation="MYNLIKDVKKLNPLVIHYTNNVTINDCANVTLAVGASPLMSFSY
                     EEVEEMVSVANSVVINIGTMNSNMLDLFLLAGKAANKYNKPVVLDPVGVFASKARAEL
                     TSRLLNEVKFSVVKGNVSEIKFIGGFNVKGKGVDSFDEEEDSTEIIRKIAEKLECVVV
                     ATGKIDIITNGKGTYKINNGTDKLKGITGTGCMTASLIASFMAVTENILEAATMGVLT
                     MSLSGELANLNNPPIGTFKENLMNAIYQMDIDTLSKNSNIEFLN"
     gene            50006..50638
                     /gene="thiE"
                     /locus_tag="ACOIAF_000623"
     CDS             50006..50638
                     /gene="thiE"
                     /locus_tag="ACOIAF_000623"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HGZ2667650.1"
                     /translation="MIDKESLKKCLKLYLVTDSEMLKGRDFYKCLEDAISSGITTVQL
                     REKNASGREFLRKAMKLREITKRYGVKFIINDRVDIALICDADGVHVGQSDIDVREVR
                     KLIGNNKILGVSARTLEEAICAKNDGADYLGVGSIFTTSTKLDAKSASFETVKEIKEK
                     VDMPFVLIGGINLDNIDKLKCLESDGYAIISAILKAEDISKEVEKWTLKI"
     gene            50777..51079
                     /locus_tag="ACOIAF_000624"
     CDS             50777..51079
                     /locus_tag="ACOIAF_000624"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGZ2667651.1"
                     /translation="MIRILLVCVGGMSSTLLVNKMEKDAKKRNIDCKIWAVGEGDIKS
                     ELDNFDILLLGPQLRFMLDDVKSIVGDRAPVSIIDMVNYGTCNGHAVLNSVLEILK"
     gene            complement(51145..51894)
                     /locus_tag="ACOIAF_000625"
     CDS             complement(51145..51894)
                     /locus_tag="ACOIAF_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2667652.1"
                     /translation="MLNILWRSMKWRFKNPISFVVTILQPFLWLVLYSSIANQTMNNI
                     NINNYTAFILPGIIVLVVFSSCSSGGIINFIMKNSGSFYRVLIAPISRYSIVLGQLLE
                     AILVSFIEVTILCIVSIFFSVRIESGIGGILLMIVLIFMTAFFLSSLAYSISLLLPNE
                     IVYETIMTAIVLPIFFLSSALFPIESLSGGLKVAVMLNPFTHVINALRSLIFGETILI
                     GDILPFILLFLILCCSSFSLAMWRLKKEMVS"
     gene            complement(51888..52850)
                     /locus_tag="ACOIAF_000626"
     CDS             complement(51888..52850)
                     /locus_tag="ACOIAF_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGZ2667653.1"
                     /translation="MLAIEIDNLVKEYKNGVKALNGLSFNVNAGEIFSLLGPNGAGKS
                     SLINILTTFYKPTSGNVKMFGKDLVDNSSWIRTQIACVAQQISIDEHLSLMENMVFQS
                     KMYKVEPQIAKQRIDSLIDKFDLSSYLKYPTSSYSGGVKRRLDIAMNMVSSPKILFLD
                     EPTVGMDVDSRKSMWDMLLKIRDEYGTTIFLTTHYLEEAEQLSDNICIMKNGKDLAQG
                     TPSSLRSYIRQNILRITFHNTEDIKKYKDSIKSTGLVKFMSVRENSIFISVNDSRTAF
                     TLINKWLLEHDIEFDAIEIVEPSLEDVFLALTSSKKSLKEEWEC"
     gene            complement(52854..53312)
                     /locus_tag="ACOIAF_000627"
     CDS             complement(52854..53312)
                     /locus_tag="ACOIAF_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HGZ2667654.1"
                     /translation="MNCKIEIKNIESVTVATMRYNGPMTEATKYFPNVFKAIKGKSNG
                     APFFCYYDVDQKTGVAEMELCVPTAETPNRMGITTKEFPKTKALFFTHTGSYSNLPKT
                     YEMIFKYIHENNIKIQTPWREVFIKGPGMLIKGNPDNYITEIIFPLKEEE"
     gene            53668..54024
                     /locus_tag="ACOIAF_000628"
     CDS             53668..54024
                     /locus_tag="ACOIAF_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGZ2667655.1"
                     /translation="MNFELDNTTPIYLQIVKYIKRQIVTGELKPGETIPSRREMALNL
                     KVNLNTVQRAYKEMGDMNIINTFKNYQSSVTVDENILKNLKLELINESLSVFIEDMKA
                     INVSKEEVLKIIEDKY"
     gene            54096..54812
                     /locus_tag="ACOIAF_000629"
     CDS             54096..54812
                     /locus_tag="ACOIAF_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2667656.1"
                     /translation="MIEIKNVSKTYKRMQGLKIRKIEALKNVSFNIEKGKITALLGIN
                     GVGKSTMLKAIAGLINIDSGEIRIDGEKINEKVYNKLAFVPDVQSHFSNTTIKETFEF
                     MEIFYSKWNKEKSKEMMDIFKLDEDEIIDNLSKGNIARVKLILGFCQDPEYILLDEPF
                     TGIDLFKREEFIGVIAQYMEENQAIIITTHEIVEIESLVDEVVILDEGQIITSFNAEE
                     LREREGKSILDKMREVYKNE"
     gene            54805..>55452
                     /locus_tag="ACOIAF_000630"
     CDS             54805..>55452
                     /locus_tag="ACOIAF_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2667657.1"
                     /translation="MNKLLYLINVDLRRSNKFYIAYISFFSLITLSLNLFEINKFKNS
                     KFIMNLAIEDFGGIFYGLGILSNLGFIQSILFFGILGLFIYYIFMWKREFFSNNQSIY
                     TLMMLPQNKFKICISKSIALITMIYGFLISQTATLFICKYIFNFIFRNMPIINMNFAK
                     DLYYLNLKSIPIDFISFMAIYVFLLLIAISIVNCCILFIFSYINKFYVILITLLII"
BASE COUNT        21910 a         5800 c         9637 g        18105 t
ORIGIN      
        1 atagttgttt tgctatttat tatttgttta atatttctaa tttataaaaa aaaaattata
       61 aattttaaaa attataaatg acattttaac tagaattaat tgtaaaaaac actatataat
      121 actcaatttt caacattcta tacaattata cagaaattat acagcataat aatcaccgaa
      181 ataacaaatt ttaaaaatta agcaaaactt tttgtcgaag ttcaacgtct atatatatgt
      241 agtgtaaatt tttgggataa aatactacaa aaatataaat gtaaaaatat agtaatattg
      301 gaggtcgaac gatgagaaat aaaaagaaaa aaataaatag aaagaagttg tatttgcttt
      361 taagtgctat tgtagtgtgt ctggctttta tagtagttgg tgttcgcgtc agctctttaa
      421 atatgaagga aaatgaaatt agtagaaatg caaaactgtc aagttctact attacagata
      481 tagtatctaa tacatcaata gaagttagta aagggccaag taaaagttct ggaaaaatag
      541 catacataac tatagatgat ggtccatcta aatttactga ccagatgata aaaactttaa
      601 ataaacacaa tgttaaagca acttttttca tgatagatgg gaatatgaag gaatatccac
      661 agcaagttaa aaatataatt aaaaatggga acacagcagg ttttcatagt gtatcacatg
      721 atattcacaa gctttatgtt actagtacat cagcaaaaga ggaatttgat acaaatgatc
      781 aaacttttta taagataact ggtaaacatt caaaagtaat aagaatacct tatggaagca
      841 aaccatatac accacaagcc tcttatcagg ctttagttga tgctggatat aaaatttggg
      901 actgggactt agatacagaa gactggagat ctaattcttc tcaaattgta caaaatgtaa
      961 aaaatcatat taagaataga aaaggtgaag ataaagacca acttgtagtt ttaatgcatg
     1021 agaaaaaaca gagtgcagaa gctttagatt cagttttaaa attcttatca gatgaaggtt
     1081 atgaatttgc gccagtagat caaaaccaaa taccaaaaaa ttattggtta cgtaatttag
     1141 agtagttaaa atataagaat attgattgat atttttagaa attgagaata aaaatgagtg
     1201 aggtgtaggt atattgaata aaaaacttta tataggaatg gtcggaatat tgtctttgat
     1261 gatggttggt tgtaataaaa gtttagcaaa agtaaatgat gtggaaatca caaaagaaca
     1321 gtacaaaaaa acaaaagcag ttttatctgc tactaataat tatataaatg ggcagtcttt
     1381 agatgaatta gaaaaaactt tggataaaaa aggtagaaat aaattagaaa atgttataat
     1441 atcgtttatg gtggataatg aacttttata tcaagaggct aaggataaag gactaactcc
     1501 aagtaaaagt gaagtagact caaaatatca agaactagaa gataagatga atttgaatac
     1561 aagctataag gaaaaaatgg acaaagcagg tgttgataaa gattatttaa aacaagaaat
     1621 atctagagat ttagcaatag ataaaaataa aaaggccttt gaagatagaa taaacataag
     1681 tgataatgat atggaagcct actatacaag tcataaaaaa gattttaatg tagaagaagt
     1741 aagtgcttct cagatactta tatcgacttt agataaaaat aaaaaagaag taagcaaaga
     1801 taaaaaagaa gctttgaaga agaaggcaga taatatattg actaaaatca aaaatggaga
     1861 aagttttgaa agtttggcta aaaaatattc ggatgataaa gcaactggaa aaaatggagg
     1921 acagctagga tattttacaa aggatgataa aaatgctgag tttacaaagg aagtgttcaa
     1981 attaaagaaa aatgaagttt caaatgtgtt tgaaactagt tatggatatc atatagtcaa
     2041 agttactgat aaaagagaaa gacaaaaaag ttttaatgag tgtcagagtt taataagaga
     2101 atctatttta aatgaaaagt atattgagca tataaaaaaa ctgaatgaag atgctaaaat
     2161 agacagatag aatttctaac agctcaatcc tagcctgtaa tcggagggag attttacagg
     2221 aatgtttaag agtttaaaga gaacgaaagt tgagaaatac attatcgata ataaagactc
     2281 tttttataga atagcatata gttatacgaa gaatgaagaa gatgccttag atgtggttca
     2341 agaagccatg tataaggcac tatactcagt ggaaaatatt aaagaagtaa attatataaa
     2401 gacatggttt tataaaattt tagttagaac gagtatagat tttattagaa aaaaccgtaa
     2461 atataataat atgacagata ttgaccttat agatgagact ggagagtatg ataaatatac
     2521 tgatttggat ttaagaagag ctttggaaga attacctatt gaatacaaat ctattataat
     2581 attaagattt tttgaagatt taaagattga agaagtagca ataatattgg atgaaaatgt
     2641 gaatactgtt aaaactaggt tatatactgc tttaaaaaaa ctaaaattaa aaattgaaga
     2701 gtagggataa aaagaatgac aagcaaagaa aggttaaata agttaaaaga tgagtatcat
     2761 aaaattccaa tacccaagaa attagatact ataataaatc atgaaaaaat agaaaacata
     2821 tatcgagaga aaaataaaaa agtaaataga ttaagattta aagttgcaat agcctttgca
     2881 tgtatattta cagtattagt aaatataagt cctgtatttg cagacaattt ttctaagata
     2941 cctgtaattg gagcaatagt agaagtaata actataaaaa attatagttt aaaaagtgaa
     3001 aattatgaag ctgagattga tattcctaaa atacgaggtt taaaagataa aaacttagaa
     3061 caaagattaa acagtagttt tatggaagat ggaaaaaggc tttatcatca attccaagag
     3121 agaatggaga agatacaatc atcaaagaat aaaggatata aatctttaag tttgagttat
     3181 agtgtaaaaa ataattctaa aaaatttcta tcaatagaga tgacaaaaaa tgagatagag
     3241 gcttcatcat atgtgagtaa agtacattat actattgata aaaaaagaca aatagttttg
     3301 actttgccaa tgctttttaa agatgataag tatataaaag ttataagtga taatataaaa
     3361 gaacaaatga gagaacaaat gaaaaaggat agtacaaaat cctattttat agatcaaaaa
     3421 aaagacttgc ctgttgaaga ttttaaaaca attaataagt atcaggattt ttattttaat
     3481 aaaaatgaag accttgtaat ttgttttgat gaatatgaag tagcacctgg ttatatgggc
     3541 gcagttgagt ttgtaatacc atataaagta ataaaagatt tgtaaattga atagtagttt
     3601 atatataaat cttttaagga ggacaatatg actacatata aaaaacttaa aaaatctata
     3661 tttaaaattg ctatattttc aatatgctta ttagtttcat tagtagatac aaaccctata
     3721 tttgcaggta gttcctttaa tgaaacttca aatagtgata cgataaaggt gttaaatgct
     3781 aagagtatca actttaaaca agacaattat gaagtgaatg tgagtattcc taaaataaaa
     3841 ggtttaaaag ataaaacttt agagaataaa ttaaacaatg agtttttgca agatgggaaa
     3901 aaaatttaca gtgaatttca aaataaaatg aaaaaggaag ataataaggg tcatagatac
     3961 ctaaatgtaa tccataatgt caaaaataat tctaaggact ttttatcaat agaaatgaca
     4021 aaagaagaga tagaagcttc ctcatctata actaagaagc attacactgt tgataaaaat
     4081 agaagaatac tattaaattt accaatattt tttaaagata gtagatatat tagagctata
     4141 agtgataata taaaagagca gatgagaaat caaattaaga aagatgatac aaaatcttat
     4201 tttatagacc aagataaaga ctcgccagtg aaagattttg aatctataaa taagtatcaa
     4261 gatttttatt ttaataaaaa tggaaatctt gtgattagtt ttgatgaata tgaagtagct
     4321 cctggttata tgggagcagt agaatttgtg ataccaaata aagtaataaa aagactaaga
     4381 tagatatata gaagattttt agatagactg gtaaggagag ttatatgaat atatatgaac
     4441 aagcaataag attaatagaa aataatgagg atttcgcatt tgctacaata acgtctcatt
     4501 caggttcaac accacgtgaa actggagcaa tgatgattgt gaaaaatgat tctactatat
     4561 ttggctcagt aggaggaggt agtgtagaag cagaatgtat aaaacatgca attaatgtta
     4621 taaaaaacag agaatcaatg ttgtataaat ttactctaaa caaatctgat gttgcaaaac
     4681 taggaatgat atgtggaggt actggagaaa tacaaataga ttttatagat agtaaattaa
     4741 aaagtaatat agaaaaattt aataagagac taaaggaaaa tacaagtaaa gcatatatat
     4801 ttggtgcagg tcatatttca agagatgttg ctgtgatact atctctttta gaatttagaa
     4861 cagtagtaat agatgataga gaagaatttg ctaatcatga aaggtttcca gaatcagaag
     4921 tagtagtttt agattctttt gaaaacatac cagattttcc aactgacgaa aatagttata
     4981 taattatttt aacaagaggt catctatatg attcaagtgc tttagaatgg gctcttaaaa
     5041 gagaggctgg ttatataggt atgattggaa gtagaacaaa aattggattg acttacgaaa
     5101 aactcatgaa aaaaggtttt aagaaggaag aattatcaaa agtacatgca ccaataggca
     5161 taaaattgaa tgctcaaaca ccagctgaaa tagctgtatg tatagctgct gaacttataa
     5221 attgtagagc taataaggaa aaataaacta atgaacacag ggagtaaaga tattgatact
     5281 ttaaaggtta attttagagg gattgaaata aaagcagttt tagtatataa aaatagaaaa
     5341 aatatttcta taaaaataga tccatttggc aaaataactg taatgagtcc tcctaaaata
     5401 tccaaaaaaa taataaaaga tataattatt gaaaaaggag actggatact taaaaagtca
     5461 gaaaaatgta aaggaagaga ggaagtatat aaacaaagaa tgtttataac tggcgaaaaa
     5521 tttctttatt taggtgaaga gtaccattta gttataaaag aactgttgag agattctgtc
     5581 aaaaaaagta actgtagaat aactattaac gaatatcaaa tagttgttca gacaaatgat
     5641 acaagtacag attttattaa aaaatcttta aaatcttggt ataagatgga aagtgaaaga
     5701 atagtattag agagaataga ttttcttaaa ttaaattgtg aaataatgaa gcaattagtt
     5761 ccagcgagtg ttaaggtaaa agaacaaaat aagagatggg gaagttgtac agctcaaaaa
     5821 aatatttata taaactcaaa aatatctatg gcaagattag atgtaattga ttatgtaata
     5881 atacatgagt ttagccattt agtacatatg aatcactcta aaaaattcta tgatttagta
     5941 aaatccatta tgcccgatta taaagataaa gaaaattggc taaaaataaa tggatataaa
     6001 ataataatct gaaaacgatt attccaaaat ataggcgtaa agtataaaag aagtattatt
     6061 gtgaaaaaaa taaataatct gaaaatttta aaaaaagtca ttgacattgt aaatgatagg
     6121 gaatataatc tatattaaca acaaaacaga attaaaatat aaaattaaaa aattaaaagc
     6181 aatgacagag acaacaatac taatagtgtt atccacagag aaccaatgtt gctgagaatt
     6241 ggtgttaaca gtagtataat gatcactctg gagcttttgt actgaatatt tttagtaagt
     6301 acaaacgtat gtcaacgtta catgactaag gttaaggtaa ttaattaccc gacctgaaaa
     6361 aagaggggta gatgtatttt ttatatctac aaataaggtg gtaccgcgga atataacttt
     6421 cgtccttata agtaatattt atattgctta taaagatgaa agttttttta ttttaagcga
     6481 tataaataaa tttaatccat ataaaattaa taaaaaaata ataaaaataa ggagggattt
     6541 ttatggaaaa tactgtatat gctaaattaa accaaggaat agattctttt ttaagagtgg
     6601 ctatgactct aagaagaaga gaggttgata tacaatcaat aagtatgact gtagataata
     6661 tgaataattc aggtataaaa ttaatagtaa atgaagaaaa gacatcttta gattctgtct
     6721 taaaccatat gaaaaaacta catgatataa gagagataac tgttgaaaga atgagacatt
     6781 aattaaatat attttataaa actaaagata aatatgctta ttttaaaata aaaaactaaa
     6841 gataaatatt aaataagaaa aatcttagat taaaatataa gtgtcattga atttattagt
     6901 ttatcacagt taataaattt attagtttgt tgtagctaat aaaaactgaa aattctaaaa
     6961 ttataattgt aagattatat aaatttagaa tttaaatctg ttgaataata ttaaaacaat
     7021 gataatgagt atgaatttct aataaaaata aatatcataa aaataagaaa aagtattggg
     7081 aggaaatgaa aatggcaaga atgtattatg aaaaagatgt ggatttagag gttttaaaaa
     7141 ataaaaaggt tgcagtttta ggttatggaa gtcaaggaca tgcacatgca caaaatctta
     7201 gagataacgg tgtacatgta atgataggtc tttatgacgg aagtaaatca gcacaaaaag
     7261 caaaagaaga tggatttgaa gttaagagtg tagcagaagc tacaaaagaa agtgatttga
     7321 caatgatgtt aatgcctgat gaaaaacaaa agaaagttta tgaagagagt gtaaaggata
     7381 acttaaaaga aggtcagaca ttagcttttg ctcatggatt caatatacat tataatcaag
     7441 tacaaccacc agaatttgtt gatgttgtaa tggtagctcc taaaggacct ggtcacttag
     7501 ttagaaatgt atttacaaag ggaagtggag ttcctgcact atttgcagta tatcaagacc
     7561 acactaaaaa agctacagag acagttttag catatgcaaa agggatagga gcaacaagag
     7621 caggtgtttt agaaactact tttaaagaag aaacagaaac agatttattt ggagaacaat
     7681 cagtactttg tggtggaata agtgaactta taaaattggg atataaaacg cttgtagatg
     7741 caggttatca aaaagaagta gcatattttg aatgtttaca tgaaatgaaa ctaatagtcg
     7801 accttatata tgaaggtgga tttgaaagaa tgagatatag tataagtgac acagcagaat
     7861 atggagatta tgtatctggt aaaagagtaa taactgatgc tgcaaaacaa ggtatgcaaa
     7921 atgttttaga ggatatacaa aatggaaaat ttgcaaaagc gtggattaaa gaaaatgaag
     7981 aaggacgtga aaacttccta aaaactagag aagaagaata taatacagaa attgctgaag
     8041 ttggtagaaa tctaagaagt atgatgtcat tcttaaaata aatatatatt aaatggatta
     8101 gtttgaggga aagagtggtg tatagtagtg cgtatgaatg gagcaaaggt aattttagaa
     8161 tgtttaaaga aagagggaat tgacacaata tttggatatc ctggtggagc cgtgatacct
     8221 ctttatgatg ctctatacga ttattcagat gattttaaac atataaggac atcccatgaa
     8281 caaggtttag ttcatgctgc agatgggtac gcaagaagta ctaatacagt aggagtttgt
     8341 tttacaacat ctggtccagg agcaacaaat gccataactg gaatagcaac agcttttatg
     8401 gattcttcac ctatggttgt aatttcaggg caggtgccaa ctagtttatt aggaaaagat
     8461 tcttttcaag aaattgacat aactggagca acattatcta tgacaaaaca taattattta
     8521 gttagaaaca ctaaagaatt agttcctaca ataaaagaag cttttagagt tgcaaattca
     8581 ggtagaaaag gacctgtact tgtagatgtt ccaaaagatt tatttttagc agaaatggat
     8641 tttagtggtg aggactatga tttatgtcaa atagatgatt atatggatta taagagtgat
     8701 tttgatctag atgatgaaac taatattaag cttttaaatg aagcaataga tatcataaaa
     8761 gaatctaaaa agcctgtaat atatgcaggt ggaggtgtta aatcttcaga cagtgaggaa
     8821 attcttgaaa aatttgcaac taagatagat acacctgtac taaatacact tatggggctt
     8881 ggaaatatag atagaaagaa tgaactatct cttggaatgg ttggaatgca tggaagtaga
     8941 gaaagtaatt tagcactttc taattcagat ttagtgatag cgataggtgc gagatttagt
     9001 gatagagtga taagtaaaag ttctgaattt gctaaaaatg caaaaataat acacatagac
     9061 atagacccat ctgaaataag taaaaatata gaatctaatg tatctttggt tggagatgta
     9121 aagctagtcc ttagtttatt aattgaaagg gttgaaagta agaataatag caattggaaa
     9181 gaagagataa agagatttag aaagagtgag ggtgtacaaa catatgaatt tcatccacaa
     9241 aatatactta aaaaaataaa tgaaaaatat gaaacattaa agaaacccac tgtggtagta
     9301 actgatgtcg gtcaacatca aatgtgggct gctaagtact ggaattttaa aggtaataaa
     9361 agttttataa catcagcagg gttaggaact atgggatttg gtttaggtgc agctattgga
     9421 actaaagttg gtaatgtaga taaaaatgta gttttagtca ctggtgatgg aagttttaga
     9481 atgaactgta atgaactagc tacagttgca aattataatg tacctatgct tattttacta
     9541 ctcaataata gaacattagg aatggtaagg caatggcaaa agttattttc caatcaaaga
     9601 tactctcaaa ctgacattaa tgaaaatgtg gattatgtta aactcgtaaa tgcatataat
     9661 attgatggat ataaggtatc cagtatggaa gaattaggaa aggcgctaga tatgatagat
     9721 tttaataaac ctgtattttt gcaatgtgat atagataagg attatgatgt ttatcctatt
     9781 gtagcaccta atgatgcatt ggaaaatttg atatgtaact aaaacttata ctgaatattg
     9841 ataaaatttt ttattaaatt tttattggta ttcagttttt gttgtttaga gatagattgg
     9901 cattttttat tttttcaaat aaatcaatga ctatataatt gagaacttat taaattttaa
     9961 ttatacaatt tggaatagta ttacaactta agtaaataat aattttgaat aataatttac
    10021 ggaggtattc atatgtttaa acacgataaa gcattattaa aagaagtaaa agtagaaaga
    10081 ccaaatcctc aatatgctgt tttaatgcaa gaacagttgg gtggtgcaaa tggagagtta
    10141 aaagcagcta tgcaatactt gtcccaaagt tttagaataa aagaccctca aataaaagat
    10201 ttatttcttg atatagcagc agaagagctt agccatatgg aaatggtagc acaaactata
    10261 aatttattaa atggacatga tgttgattat aattcagtta atacaggcga aatagaaaca
    10321 catgtattaa ctggtctatc accagtctta ataaactcat caggagctcc ttggactgct
    10381 aattacgtta cagttacagg ggatttagta gctgacttac tttcaaatat agcttctgaa
    10441 caaagagcta aggtagttta tgagtacttg tatagacaaa tagatgataa atatgtaaaa
    10501 gaaacaatag atttcttact taatagagaa gaagcacata atgctctatt tagagatgct
    10561 ttaaataaag ttaaagatac aggttcaaat agagattttg gagttacaga agattctaaa
    10621 ttatatttcg atttgtctac tccagggcca aatcacgata ctaagataga tattaatcct
    10681 ccttcttttg aaaaaccttt aaaaaaataa tttgatattt tttatgttgt tttgttttag
    10741 tagttataaa aattatttat tagaagaatt aattatatga aaaattaaat atgtatagtt
    10801 aaaaatgggt atacacttat tataagtgta tatctatttg tttattgcaa aacatataat
    10861 aaaataacaa atgaggtggt attttggaca gtaatagaaa acctaataat ctaataaatg
    10921 aaaaatctcc atatctttta cagcatgctt ataatcctat aaactggtat agttggaatg
    10981 atgaggcttt taaaaaagca aaagaagagg ataaaccaat atttttaagt gtaggatatt
    11041 caacatgtca ttggtgccat gttatggaaa aagaatcttt tgaagatgaa gaagttgctg
    11101 aaataatgaa tagaaacttt gtggcaataa aagtagataa agaggaaaga cctgatgtag
    11161 atagcgtgta tatgacagtt tgccaagcta tgacaggtag tggtggatgg ccaatgacca
    11221 taataatgac accagataaa aaaccttttt ttgcgggaac atatttccca aaatattcaa
    11281 ggtataatag acctggagtt atagatttat tagagaatgt atctgaaaaa tggaatacaa
    11341 gcagggatat attaataaaa tctggagatg aaataataga agctcttaag gatgattttg
    11401 gtgtaaaaaa tactgagggt gatttatcaa aagagatgtt gagttcatca gttagagtat
    11461 ttaaggctat ttatgatgaa aattatggtg gttttggtaa tgctcctaaa tttccaagtc
    11521 ctcagaatct tatgttttta atgaaatatt atagtataga aaaagataaa gatgtgttaa
    11581 aaatggtgga aaaaacttta gatggaatgt atagaggagg cctgtttgat catattggat
    11641 ttgggttttc aagatactct actgataaaa agtggttggc tcctcatttt gaaaaaatgc
    11701 tatatgataa tgctatgctt actatagctt ttttagatgc gtataaaata acaaagaaag
    11761 agttatataa agaaattgct ataaaaacta tagattatgt agttagagag atgaaggata
    11821 aagaaggtgg gttttattca gcacaggatg ctgatagtga aggtgaagaa ggtaaatttt
    11881 atacatttaa tcctcttgag ataattgaag ttttgggtga agaagatgga attttcttta
    11941 ataattattt tgatataact agtagtggaa attttgaagg caaaagtatt cctaatctta
    12001 taaaaaataa agaatatgaa agacataatg aaaagatagc tgaccttagt aaaaaggtat
    12061 ttgaatatag aaaagaaaga acatctttgc ataaagatga caagatactt acatcatgga
    12121 atgcattgat gatagttgct ttaacaaagg catatagtac tttaaaaaat gacatatatt
    12181 tagagtattc aaataagtgt cttaacttta taaataataa tcttgtaaat gagtcaggta
    12241 gattattagc tagatataga gatggaagtt ccgactattt agcatattta gatgattatg
    12301 cttttttaat ctgggcgtat atagagcttt atgagtcaac atttaatatg aaatatttag
    12361 aaaaggcttt aaatttgaat gaaagctgta tcaacctttt ttgggattat gaaaaaagtg
    12421 gattttatat atatggtaaa gatagtgaaa atttaatagc aagaccaaaa gatttgtatg
    12481 atggagctat tccatcagga aattctgttc aattatacaa tcttataaga cttgcgaaaa
    12541 ttactggaga taatagactt gaagaaatgt catataaaca attaaaactg tatgtggata
    12601 atgtaaagag ttctccaact ggctacagct tttatatgct ttcattgatg tttgaacttt
    12661 attctactaa ggaaattata tgtattttta aggaagattc tgatttaatt gcatttaaag
    12721 aattgataag tgaaaatttt ataccaaatg caactttttt agcaaagaaa tacaatgaag
    12781 aaaacactat tataggtttt ttaaataact atatattgaa ggatgataag actagttatt
    12841 acgtgtgtca aagtaatagt tgtagtcaac caattaatga tttacaaaaa ttaaaagata
    12901 tgattttagg agtagaataa tcataatagc gaaaaaggag atgctttaaa ataaaagtct
    12961 aatcatctcc tttttttgta caaaaaacag aaaaagagtt cctaagaaca cctaatctat
    13021 gtaatgattg ttttgttaaa aaataaaaat gacattatag ttatataaaa tattgtacta
    13081 caaaaaagta gtattataga tgcgatagta ttaatcgttt ttatttactc tctttcttct
    13141 ttttgcaaat tcttcaagtg cttcagattt aattttatct atttcttcat attccataaa
    13201 attagcatct tcttcaaagc catcttgttc ttcaccatta aaacgataca ttttttttag
    13261 taaactccat aaggtctcta attcatcttt ggtaaattcg tgaaatacat ctgccataaa
    13321 gtttattcca gtcctactac atgtcaccat aacctttttt cctaagtctg taactttaac
    13381 attaattgct ctcttatcat gaggacttgg aattacatca acatatccat ttttttcaag
    13441 attagcaact agtctattta tattttgttt acttgtaccc atttttcttg caatattatt
    13501 taatgttgtt tcttcctctg gtaaatgaag aattgataaa atagtcatat actgtcttga
    13561 agtaagtata ccaaaatatt tatctccttc tatttgtatt ttgtttgtaa gggtaaaaag
    13621 agttgcatat gtttgattca ttaaaaagag ctcttttagt tcatttgaat aatccatgaa
    13681 atatatctcc tttattttta gaagttgttt tgtcgaaaac agaatatcat gaattaattc
    13741 taatgtcaat atggtgtcaa aatatattaa agtgttactt ttttatgtaa ctattaatta
    13801 attaaattat tgtataattt tatgtaaaaa cttctatgta ccaatgataa cttctatgag
    13861 gaattaataa ttcttaaatt ttttagaaag attttgactt gaaaatattt gtaatataat
    13921 atataaatta atatactgaa taaaatcaat ctggagggat aattatgaag tgtacaaact
    13981 ttactttcaa aggagaggaa ggtttagata tatatacata taaatgggaa gatgaaaata
    14041 taaaaaaacc aaaggcagtt atccaaattg cacatggaat ggctgaaact gcacaaaggt
    14101 atgagacttt tgccaaggta cttactaaaa atggttatat agtatatatc aatgaccata
    14161 gaggtcatgg aaaaactgca aagataatag aaaatgttgg acatttagct gaaaaagaag
    14221 gatttagatg tcttgtagag gacatgtata ctttaacaaa tattataaag aaagaaaatg
    14281 aagatctgcc aatttatcta tttggacaca gcatgggttc atttgcaagt caaagatata
    14341 taatggatta cagtaataat ttatctggtc ttatactatg tggttcaaat ggaaagcaag
    14401 gaatcattct aaatcttgct catctgataa tcaatcgtga gattaaaaag tatggaagac
    14461 gttctaaaag taataaaata aataatttga tatttggtgg cgaaataata agaagaaatg
    14521 aaaagactaa gtttgactgg ttaagtagag ataaagaaca agttgaaaaa tatataaatg
    14581 acccattttg tggagtagtg tgtagttgtg gcttttttta tgatttagtt caagggttaa
    14641 aagaaattga ggataaagaa aacttaaaaa aagtgccact tgatatacct atttacataa
    14701 tatctggaga taaagacccc ataggaaaaa atggtaaagg agtcttaaga ctaagggata
    14761 gatatataaa attaggagta aaagatgtaa cttgcaaact ttacaaagat ggaaggcatg
    14821 aattgttgaa tgaaataaat agagaagaag tttttgagga tataatttgt tggttaaata
    14881 ataaaataga aatattgtaa taaaaaaata taatgatata tttgctttaa atttatttat
    14941 atataatatt tgaaaagtgg attatgtata tgcaaagtag aaaagagaga gcccagaagt
    15001 gattttttgc tataatgcca tttattgaat aggtgggtgt tattataaat aaattattaa
    15061 cactcagtat tattatcatt gtatatggaa tttattttat ttaaaattcc ttgaaagttt
    15121 aaaagtttaa atatagtaag ttgtaataaa gagaaaatct aaatatttaa atgctattta
    15181 atgtgaatct attaattagg ttatacttaa acttaattaa tagattttat atttcaagac
    15241 aatgagatag ggttttagat taaaatataa ccaatggtta aattacagac attttgttaa
    15301 ctttccctcg atttactagt aaatattttt ttaatatact taaattacaa cagatgtact
    15361 agggaggtat atatgcaaat taaaataggt aaagtcatac agcgtcttag aaaagaacga
    15421 aatttaactc aagaacaatt agcaaagttt ataggtgtgt caacaccagc agtatctaag
    15481 tgggagagtg gaaattctta cccagatata gaattattgc ctttattagc agactttttt
    15541 aatgtgtcta ttgataagct tttaaattac aagattgatt taagtgaaga agaagtaatg
    15601 aaaatttata aagaattaga atcaggcttt gctagaattg aaattgattt atcaacagaa
    15661 gaaccaaaag aagaatttag acaagactta gaatctgtaa aaaagctttc caatatgtat
    15721 atagagaagt atcctaaaag ctatttattg aagctaagga tatgttcgct gtatcagatg
    15781 tattcatata aatttggtaa gagtgaactt aatgacagag taaaagaaac aacgaatatt
    15841 cttgaagata tagtgagaaa tacagatgat atccaaataa aagaaacagc tcttattata
    15901 ttatcaaacg catattgtat gttggaggat tatgaaaagg cagagttgta tttaaatatg
    15961 atacataagt caatagggga tacaagtgtt aatttagcta tgatatattt aaagcaaaat
    16021 agacttgaag aagcagaaat attgcttcaa aataaattgt ttagtaatgt atttaatata
    16081 agcatggatt gtaagggaat aattaatgta tacaaaaatc aatacaagga attaaagaaa
    16141 aaattggaga atagaaattt taataaaaat gcaattgaga gagaaatgga gtatataaaa
    16201 aataagttgc tggggtatgc aaatctttct ttagaaataa aaaaaatgct cagtgaagat
    16261 aaaggtgcat tttttagtat gtatatggat tatatggaat tatctctaat ttttttattt
    16321 tttaacatga aagaagaagc aaaaaaagcc ttatatagtc taaaagaaat attagaaaaa
    16381 tatcctatac atgaaagttt ggatgtaagt caaatgagat tttttgataa ggtagagtct
    16441 aaaaatttat atacttttaa tatatataca aacttgttaa ttgtacttaa tgatgatagt
    16501 tataatgagc ttagagaaga accaattttt aaggatgtaa ttgaaaagat tttagatatg
    16561 gaaaaaatgt tgaaaaataa agaataagta aaaatttaat ataggattta aagattaatc
    16621 aaattaaaag aaagtgtttc aaaataaaaa tttgaattac cttttttgtc ggtcttaaac
    16681 agttatttag aaaaatagaa taaagtttgt tatataaaat gtaattttgt ctataaaatc
    16741 tcacctgttt tcttaacttt aagcgtgaaa aagaggatgt atcatgaatt ttttagttca
    16801 tgatgcatcc ttctttgtat gaaatccaat atatctttat tatttaaaca atggaaaaga
    16861 gtcttatttc tattttgaga catctctttt atttaattat gaagttttat acagttcaat
    16921 aaataggaat tatatttaga aactcatgac attatttctt agatagtgat taaatttatc
    16981 atattccatt ttagataact tttctgggta agacattaca atcaggttat gcccattaaa
    17041 tttttcatgg tatggtggat gaatgtgttc ataattattt gtcttatatt ttactctttc
    17101 atagaacgcc ttgcgataaa ttgaaatctc atcaattgga aggtcaattt ctaaaataat
    17161 agtttttcct ttcttattta aaagctctaa cgctctttgc ccatatctat tattgcgcat
    17221 ttctggaaaa atgcagaaat gttctacata gataaagtca tctgtttccc aacataaaat
    17281 tatgccgaca aactggtttt tatcataaat taaattgaat tgatattctt tatttttcaa
    17341 aatttctgct tgcaaagaag attttctttg ttcatgaaat gggaaactaa ttttatataa
    17401 ttccatagcc ttgtaatata atttattttc actggttctt aaccgttcaa atttcacttt
    17461 actcctccta atcacgaaaa ttccaattta tttatgagtt cattttacca tagtcagtat
    17521 ttgcatacaa tgcgttttaa gttgagtgtc atttctttct tttatttatg cttatatctc
    17581 atagtctaag agataattgc ttaagacata actaaaaact attatattaa gctttgataa
    17641 tctaaaaaca gtatatttat ggacttttct aggtctaaaa gtaatcttaa taattttaga
    17701 agttacaaaa aagttacata aatttggaaa gctataatat acatatataa tataaggtta
    17761 aaactgtaat gaataatatt aatttaaaat ttgaacatgg aaggagatta ttatggagaa
    17821 aagagaaaag aggataataa tatcatcttt attaagtgta tcaattttaa tgggtttggc
    17881 aagtatatat tctatattaa ataaagaaga cataagttta accgttaaag gtcaagagca
    17941 aaaagtatct tcctttaaga aaacagttga ggaacttttg gatgaacagg gtgtaaagta
    18001 taattctgaa gataagatta atccaagttt agatacagag ttaaaagatg atatgaaaat
    18061 aaaagttgtt aaggtaacta aaagtaaaaa agaagagatt aaaaaaattc catttgatac
    18121 aaagcatgta aatgatagta atttgttaaa aggaaaatct aaagtttatc aagaaggtca
    18181 agaaggagaa aaaaaactag tctataattt aacttaccat gatggaaagt tagtcaaaaa
    18241 agtcttatca aaagaagtaa tatctaagga gccaactaca aaaattataa aatatggaac
    18301 taaagaaaaa gtactaatag catcaagagg agcaaatata agaggaggca aacatatgaa
    18361 agtggttgcc actgcatatg caggagatac aataacatct actggtacaa ctccaagatg
    18421 gggtgttatt gcagttgacc cacgtgtaat accatatgga acaaaagtat atatacctaa
    18481 actgggtatg acttttgtag cagaagattg tggaggtgcg attaaaggta atagaataga
    18541 catttttatg aacagtgagg gaaaagcttc aaattgggga aggaaaagta tagatatata
    18601 tctacattaa attagtgaat agaataactt tacttgaagg acaaaaaagt ataaaatatg
    18661 aattaactaa actgttagat tattcttaaa acataaaaat atatctaagg ggggataaga
    18721 tgaaaatagt ctgcttagga gacagtttaa cgtatggatt tggagtatca cgaagtaatt
    18781 catggacaaa cattgtaaat aaagagactc gattagaaat agtaaataag ggtataaatg
    18841 gtgatacaac aagtggtatg ttagctaggt ttaatgaaga tgttgtaaaa aattctcctg
    18901 atatagtttt tataatgggt ggcaccaatg attttattgc tggtgcggga aatgaaatta
    18961 ttaattctaa tataatggct atggtgcatc agtcatattc aaaaaatatt atacctataa
    19021 taggtatacc attaaaacca gatattccaa atgttaggaa agattggagt tgttttactg
    19081 attttaatat agtatctcaa aacttagaat catatagtta ttggataaaa aaattttgta
    19141 tgacatttaa tactaatttc gtagattttt attcagaata taataaaaat atggaactag
    19201 aaggatataa aaaattatat tttgatgggt tacatccaac caaagagggt catagaataa
    19261 tggcagacat ttttattaat tcaattaata agtatataga agagttatag attacattaa
    19321 aatgcttgtt tatgcgtcag ttgcaataaa tacatacagt agagaggggt ttattatgga
    19381 ttatactaaa acttatgaag agtggataaa gggttcatat tttgatgaag atacaaagct
    19441 agagttggag aatataaaaa ataatgaaaa agaaatagag gatagattct ataaggattt
    19501 agaatttggg actgcgggtc ttagaggaat aattgaagca ggaacgaata gaataaataa
    19561 gtacacagtt agaagagcta cttttggatt ggcaaactat atattagaaa acacaacaaa
    19621 agaagaaaca agtagagggg tagtaatagc tcatgataat agacacaagt ctagacaatt
    19681 ctgtatagaa tctgcaaata ctttggcagc atgtggtata aaagcatata tatttgatag
    19741 tttgagaact actccagaat tatcttttgc tgttagaagc ttaaatgcaa tcgctggtat
    19801 agtcataaca gcaagccata atcctccaga atataatgga tataaggtat actgggaaga
    19861 tggagcacaa gttatgccag agattgcaaa tgcaataaca gagaaagtaa atagtataca
    19921 tgattatagt acaataccaa ccttaacgga agctaataaa aatttggtag ttttgttgga
    19981 cgaatcacaa gatactaaat ttatagaagc agtaaaaagc caaattataa gaaaagattt
    20041 agttaaaaat gtaggtaaaa gctttaagat agtttataca cctctttgtg gaacagggaa
    20101 tgttccaata agaagagcat tgaaagaagt tggttttgaa aatataattg tagttccaga
    20161 agaagaaaat ccagatccaa attttgcagg gcttgattat ccaaatccag aagagaaaaa
    20221 agctttaaat agaggtatat tactcgcaaa agaaaaagga gcagaccttg taatagcaac
    20281 tgacccagat tgtgatagag taggtgtagc agtaaaaact actacaggtg agtatgcttt
    20341 acttacagga aatcaaattg gtggtatgct gacacattat ataatagaag gattaaaaga
    20401 aaataacaaa ttaaaagaaa atcctacaat aataaaaacc atagttacat cagaatttgg
    20461 agcagatata gctaaagcaa ataatgttga tgtattaaat gttcttacag gttttaaatt
    20521 tattggtgaa aaaataaaat tatttgaaca aaataaaaat agaagttatg tatttggata
    20581 tgaagaaagt tatggttact tagttgggac tcatgctaga gataaagatg gtgtagtttc
    20641 ttcattgtta atatctgaaa tggcagcatt ttactattca aaaggtatga gtttatatga
    20701 tggtttaata gaactttata aaaagtatgg atttttcaaa gaacagacta tatctttgac
    20761 tttaaaaggt atagaaggtg tagaaaagat aaaagaaata atatcttatt ttagagaaaa
    20821 tcaaattgat tatataaata gtattaaagt agttgataaa aaagattata aaaatggtat
    20881 agataatctt cctaaatctg atgttttaaa atactttttg gaagatgaat cttgggtagc
    20941 tataagacca tctggaacag aaccaaaact taaattttac atagctgtaa aaggagctag
    21001 tgatattgaa gcagataaaa agattcaagg tttgaaaaag tatatagatg acatggtcga
    21061 aaaattaaaa taaaatatta ttattttata agataatttt gtaataatat ttatttagct
    21121 gaaaattata atttactttc attgagtaga atatggaaat gttagtagaa ttgttatttt
    21181 cagcttttat ttgtgttatt tttacttaaa taattatatt atagaatgaa tctgagtata
    21241 attaatattg tataatgaaa ttaatttgat agaaaagggg atatttatgt ttggtataga
    21301 taagaaattg attgaatata taattgcaaa atccaattac agtgaattaa gttcaaagtc
    21361 agcagaagta tctttcttct taatgttgtc catatttcct tttttaatat ttacaataag
    21421 ttctatagct tatataccga ttcttcattt aaataagtat atagctttgt ttagaaatat
    21481 gatgccagaa ggtgcttttg ctgtgttatc ttcaatcata gtttctgcaa tagacaatag
    21541 aaacctgaaa tttttagcag tcagttttgt acttactatg tggacatttt caagagcagt
    21601 aaaagctctt attaaaggaa tgaatagagc ctataaagtt aaagaaacta ggtctttttt
    21661 taaaatttta tctatatcat ttttatttac aataatgctt ttggtcctta tttttttatc
    21721 tatgatattt ttagtttatg gggaaaaaat aggttatttt atttttaatc ttgtaggatt
    21781 ggatgagata tttataaaaa tatgggatat tctaagatac actgtaggta taataacaat
    21841 aatagtgata tttacacttt tatataaata cactccaaat aaaaagttaa ctattaaaga
    21901 atctgcgcca ggtgctattt ttgctacttt tgcttggttt ttagtatcat ttttatattc
    21961 ttattataca aattactatg ctaactatga agtaatctat ggaagtatag ctgaaataat
    22021 tgtacttatg acctggatgt attttagtag ttggtctatc gttattggct atgaggtaaa
    22081 ttcaagattg tattttagaa agataagaca tgaaatgtta aagtaaagta tttaaaataa
    22141 aagagagtat ttagtattat aaatgtaatt gaattaataa agaaatattg acatttaagt
    22201 taaaaagtct agaatttttc tgggcttttt ttatttttag tataacttga caaattttat
    22261 ttaatgggat agttttaatt tataatcatc tttaaaaaga tgactattga taagtaagag
    22321 ataaaattgg taagtatcaa aaaatatact aaataaaaat tatagtccta atgagttaat
    22381 ttctgtattt tcaaataatg cagtatttag tcatgtgttt ttatatgcgc ctgtatttct
    22441 aattagcaat atattagaat aagctactgt aattagattt agaaaaaagc gacataaata
    22501 attataaaca aataataaat tacataaaat taaatttatt cacaagtata tcttataaaa
    22561 tatgttataa ttaatagaaa attgtcatat aattaattaa gtagtattat tataaataac
    22621 attgaaaggt agtggttaga atatggatac ccataataaa tatgtaaatt ttataaaaaa
    22681 tatacctgta ccttttttat attgtagaat tgtaaagaga caagaagata tagaatatcg
    22741 agtagaatat ataagtaagg gtatgggcaa agtattacaa ttagaagaag gtatttgtga
    22801 taaaaatata ctagatgtat tgcctgtatt taagtctaaa aaatatttca aagagttgtt
    22861 ctcaaatgaa gtggattgta taaaaaggta cattccgaca cttaaaaact ggataaatat
    22921 aaaaaaacag ataatagggg attcatacat aattttgtat tttggtaaaa ttgtatttga
    22981 ttatagacag ataatcgatt cctttgataa aaaggaaaaa gtagcttata ttaaagacga
    23041 agaaggcata tatatagatt gtagtgaaaa tttaatacca atattaaata acaacattaa
    23101 aacaacaaaa gatatctttg gtaaaaatga tatagaagta tggggagaga atacaggaaa
    23161 attatttaga gatgattata gagaaggagt atctagtaaa aaaagatttt tacaaaatct
    23221 ttttgaatat gaagaaacat tttttatggt tgaaaaatat tttttgtatg atgaagacga
    23281 acttttaggt actataggga tagtagacaa tataatatat agtggttaca gtaatagaaa
    23341 ttataactca aaagatttaa tgaagatgat agaacactca attccagaga atatgtttta
    23401 taaagatgtg tatggaaatt atattggatt taattcaggt tttttgaatt tagcttgtat
    23461 gaataaagaa gaattattag ggaagaatag ttataaaata tcagaagaag aagctttaat
    23521 agataagata tttgaaagtg ataagggtgt agttgaaaat aaaaaagtag ttacatttga
    23581 gttaaatata agtatgaatg atgaaaacaa gtgtattgag attacaaaaa ggccgttttt
    23641 tgatagctat ggaagtgtta ttggaataat tggaacagca agggatataa gtagaagaaa
    23701 aagattagaa gaggaaatgg ataaaactag gatggaattt tttgcaaatt tatcccatga
    23761 acttagaacg cctataaatt taatatcatc ttcattacaa gtgatagaaa aaaaagaagc
    23821 agatttaata gaatcaaatg atactttaaa aagaaatcta ggtataataa aacaaaatgg
    23881 aaatagaata ttgaggttag taaataatgt tatagacttt acaaaaatgc aatcaggata
    23941 tttagatttt aaaccagaag aatctgatat aattgcattt atagaggaga tatgtatgtc
    24001 tgtagctgat tttgcaagcc aaaataatat acaacttaca tttgatacag aaatagaaga
    24061 attttcaatg ttatttgatt cagaaaaatt ggaaagaata atattaaatt tactttctaa
    24121 tggaattaag tacaataaga aagatggaaa aataaatata tttttatatg tgaaagataa
    24181 tgtatttaat atgaaaatat ctgatagtgg tataggtata ccaaaagaaa aaatagataa
    24241 aatatttaat aggtttgaac agattgacaa tgaactttct tacagagtta aaggaagtgg
    24301 tattggactt tcattagtga aatctttggt tgagttgcat gagggaagta tatctttaaa
    24361 aagtcaactt ggtattggaa gtgaatttat agtttcattg cctgttagaa gtaaaaataa
    24421 tattgaaaaa tataatcata aaagagaaat cagcaatgaa ctaagtaaaa aattagaaat
    24481 agagttctcg gatttataag tttaaatata aaaaataaaa ttttaaaaaa gtattgacaa
    24541 ttgatttaga agggtttaaa ataaattaca acataataaa taaaaaggcg atgaatagga
    24601 agagtaaatc taattcgatt accagagaga agagtccgtt ggctggaaga ctttttaaat
    24661 tagagggatt gaaaactacc taggagctac tttctgaagt catctgcatt agtagatttt
    24721 agtaagaata gtcgttagct gcgttaaagt gtaaagtgag ttttcatgac atatgaaaac
    24781 taaattaggt ggaaccgcgg gaataatact ctcgtcctta tgttataagg atgagagttt
    24841 tttgttattt aaaaatataa acagtaatca taataattat agaaaatata aaagcaatga
    24901 ataggaagag taaatttaat tcgactatca gagaggaaag ttcacaggct gaaagacttt
    24961 ctaagtttga gaaattgaaa actacctagg agctgtggtc tgaagttact ttaaaaagtt
    25021 ttagtaagac taaccgttaa ctgcgttaaa gtgtaaagtg gattttatca ataaatgata
    25081 aaatcaaatt gggtggaacc gcgagtatag actcgtccct ttatataggg atgagtctat
    25141 ttttattgca ataaaagatt gaaaataaaa aaaaatcgga aaattataaa tttaggagga
    25201 aaataaaatg gagaataaag tgaaaattgg agtactagga tatggagttg taggaagtgg
    25261 acttatagat ataatagata ataataaaga aaaaagaaat atagaaatag ttggaatttt
    25321 agttaataat ttggaaaaac ataaggacaa aaaatattct aatataataa ctaataatat
    25381 agatgatata tttaataaag atatagatat acttgttgaa gtcatgggtg gtttagaacc
    25441 atcattatct tatataaaaa aagcattaaa caataaaatt catgttgtta cagcaaataa
    25501 agatttgtta gctgaatgtg gagatgagct tgcaaaatta gcaagtgaaa ataaagtaag
    25561 tattaaattt gaagcttcag ttgcaggagg tataccagta ttaaaaccaa taatagaatc
    25621 actagaagga aataatatag atagtataaa tgcaatttta aatggaacta ctaattttat
    25681 attatctaaa atgtatgatg agaatctatc ttatgatatg gcattaagac aagcacaaga
    25741 attaggtttt gctgaagcaa atccagagtc agatgtatta gggtatgatg ctgctagaaa
    25801 actatcaata ttatcaactc tagcttatga caatagagta tattggaaag atgtgtattt
    25861 agaaggaatt acagatatag atgaaaaaga tattgaatat gcaaaaaaat taaattgtaa
    25921 aataaaattg attggtcaaa gtaagtatga aaatgataaa gtaagtgctt ttgttagacc
    25981 tgttttagta gaaaaagata atatccttgc tagaatagac aatgagttca atgctgtcat
    26041 agtgaatgga gattcagtag gagaagtttc ttttgtaggt aaaggagctg gaagtttagc
    26101 aacaggaagt gcggtttatt cagatgttat agatataata gataatagag tttcaagtat
    26161 agattctttt actaaagata aaatacaagt taataaaata gttagagaaa agtgtggagc
    26221 acttcttaga tttaaaaaat gtaataaaga tgaaattcta aatatagttg agaattgttt
    26281 agtaaaattc gatattttga atgatgatga tgaacttgct attatggttt atgctgattc
    26341 tgaatatgaa ataaacaaca gtttatgtct aataaaagat aaaggatact gcgaaaaaat
    26401 gaataaaatg ttgaaaataa gttaaaaaag aaaaaatata agtttatatg taacatattt
    26461 tgacgatttt aatactatat agtatgagat gaaacaaaac atctcataaa aaaattaaaa
    26521 ttaaagggag agatatgtta tatggaaaat aaaaaatgtt attcagaaga ttggtatgaa
    26581 agaggagaat ctacagctaa atggttccaa aatgatagag aagaatatga aagagaagca
    26641 tatgatgaag atagagaaag aagaggttca aactgtggat gttcagattc aggagaaaat
    26701 agacctagaa actgtgaaag atttagaaga gaagctgaga taagagaaag agaagcaaga
    26761 gaagcattct gtgaatcttc agagaaaaag aaagaggcat tagcatatga atgtgaagct
    26821 agaaaattat gggaagaagc agaaaaatac tgggatgaat attcaaaata caactataaa
    26881 ggaatcgaat atttagcaga agctgctaga ttatttgatg aaggtatgga atgtgaagct
    26941 agaagaaatg gaaataatgg aggaaacaat aataattgtt gccataaatg ccataaatgt
    27001 aattgtaact gctgtagaaa ataaaaaaat taattgacaa atcaatagtt aaaatatatc
    27061 atattatgta taataaagag aaaattcaga aaaaaataat aattagaaga aatataagta
    27121 tatttattag acaaatagtg ctcagttagt gaggtgagta caatatgatt aaactcccat
    27181 cctctagctt ttgatagagg gtgggagttt ttatttattt atatatctta aatgaggata
    27241 atattgtaaa ataaatgagt atgaaattgt aaaattagga ggtaaaaaat gaaaagtatt
    27301 aaaacatcaa gtatgaaaga aaaggtagat gaaaagatat taagagagca agtaagttct
    27361 ataatagaag atataagaaa taataaggat gttgcactta aaaaatataa tgagaagttt
    27421 gacagaaata caagagatga attcagaatt actaaggaag aaataaagga agcttataag
    27481 catgtagatg acgaatttat aaataactta aagattgctg ccaaaaatat aagagaattt
    27541 gctaaagctc aaaaactaag ttttgaaaac ccttttgaaa aagaaattta tccaggtgta
    27601 atcttaggtc aaactaatat tccaatagag tcatgtcttg catatgttcc aggaggtcag
    27661 tatccattgt tttcaacagc tcttatgctt ataatacctg caaaagtagc aggagtaaaa
    27721 agaatagtgg catgttcacc aactatgaaa aatactgaaa agataaatcc taagacattg
    27781 gtagctatgg atatagcagg tgctgatgaa atatatgcaa ctggaggagt tcaagctata
    27841 gcagcattta catatggaac agaaaagatt aatccagttg acataattgt aggaccagga
    27901 aataagtttg taactgaagc taaaagacaa tgttatggac aagtagggat tgattttgtc
    27961 gctgggccaa gtgaagttct tataatagcg gatgaaactt caaatccagt ttatattgca
    28021 gctgatttat tagcacaatg tgagcatgat ttaaatgcaa gaggaatatt attgacaaat
    28081 agcttggaag tagcacaaaa agttgaaaaa aatatagaaa gtatgctaaa agatttgcca
    28141 acaaaggaca tagcatatag ttcatgggaa aataatggag agataatatt ggttgatgat
    28201 atggaagaag ccattaaaat aagtaatttt tatgcaccag aacatttaga agttgcagta
    28261 aatgagtata atgatatttg tgatagatta actaactatg gttctttatt tattggaaat
    28321 ctttctgctg aggtgtttgg tgactatgtt tcaggtacca atcatacatt accaacactt
    28381 aaagcatcaa gatatactgg tggtgtttgg gttggaactt ttatcaaaac ttgtacaaag
    28441 cagatattta atgaaaaagc tatacaatca ttagcacctg tagctgaaaa gctggcaaaa
    28501 gaagagggat tatatgcaca tgcaaaggct gctgaggtta ggtttaaaaa gtagtaaaaa
    28561 attttatttt ggcagtcgag acttagtatt agactgagat aaggaaaacg ctgagaacct
    28621 gaataaatac tatattttgt gggtctcggc gcttctttta tatctgtata taatagaata
    28681 atactatctg tgtgtttaaa tactaactta atatttcttt tctaaataat gtttatgaaa
    28741 tagttaatat ttttagttaa tacttgaaaa aaacttttgc taactgtgca aattcatctg
    28801 catttgtcaa tatagcaggg tgtcctccct gactaaaaat gtaaatactt gcttttgaaa
    28861 taaaagttgc catttgcttg tactcttctt caagattatg acgacacatt tcgtcttcct
    28921 tacttcctgt aaataaaatt ggcatttcta atttacacaa ttctttatga aacaaagggc
    28981 gtttctcgtt tgcacattgc aatagagctt ttgtatcaag attaacaaca ttctcccaat
    29041 cttttccatg acaccattca tagaattttc gtgcttgaat atcttgcttt gatttttccc
    29101 ttccagtgat tagattatct atgaagttat cattaagagt acgtccatca aagctatctg
    29161 ctataattgc ttcaactaaa tcgggacgtt ctaatccagt attaacagct gcccatgctc
    29221 ctccacttgt tccaattaag ccaactttac cacaattcaa atgttcaatc agtgctattg
    29281 tctgtaaggc ttcatcatgc catatgtcag gagaaaattg agaaactcta tccgactgtc
    29341 cattacctaa aaaatcaatt aagatacatc taaaattttc tacatataat ggcacaagtg
    29401 ctttaaacat attagaagaa ccagtattac catgcaggaa aattatcggt ttaccttgtc
    29461 catattcttt ataaaaacaa gaattacctt gatactgaaa atatgacata tattttcctc
    29521 ctttttattt gttaagttaa ttataacaat ataggtatga atgaaaaacc tcatgttctt
    29581 tcttagaata aaaatgtgcc acgcataaaa cgtgacacat ttctaattat aaattttgct
    29641 gtttaagtat tctttgtatg cttttctctg tcaaaaagta tttctctgat aactgcttga
    29701 tactcattcc attttggaaa tttatacaga tttgagaatt acgagaagct aagacttgtt
    29761 tggtgtttgt gttttcaccc caatgctttt tgtgctcttg ctttttggga atataaatca
    29821 ctttaccatc tatatattct tgcacttgct caatcagttc tttaggcagt aagtcaatcg
    29881 aattttgata gctcattttg cacctcctaa ataattattc tttaggatta gcaaaggcta
    29941 ctgccactca caattaaaaa taaaattttt tattatgtac aatagccttt gctatgcaca
    30001 agttccaatt cccataaact aaatactcct ttctggtttg aaaaattcat ctgaacattg
    30061 atttcgcttt taagatagag ttcaatgaca aatttacatt tttattatat atattcagat
    30121 agataattgc aactgtcttt gtatgaggat attttattat agtcaatact ataatatgta
    30181 tttatatcat caaactaata tgaacagttg aaaggaatat gaacaataaa acttagtcag
    30241 gaagaataat tatgaagcag acaatagaga aatttgattt taaagtttta ttgaaaattt
    30301 attgacaaat ttaacttgta aatattatac taaacttaat aaataaagtt tataaagtaa
    30361 ggtgttgtct atgaaaaata tagttggaaa accgcaaacg ataaaaaaag taaatgaaga
    30421 tttaataaaa aatattataa aaaatgaagg tcctataaca aaaccagaaa tttcaaacat
    30481 tactgaattg agtttagcta cagttaataa gattgtagag caattagctt tgaaaaatga
    30541 agttaaagta agtggactaa gtgaatctac tggaggaaga agagctcaat tatttgaaat
    30601 aaatgcaaac ttagaacaca taatagcttt atattattat agaaattgtt gtataggggt
    30661 agttgccaat ttattaggtg aaataattta tcaagaagaa tttaatataa gaatggatac
    30721 gtatgaaaac gttaaccaag atactttttt tgttatagat actctaataa aaaaggaaaa
    30781 aggaaacaat ataagggcta ttggaattgg tgtcccagga gttgtaaaaa aaggtgtagt
    30841 taaaaatata ccaactataa aaagttggga aggtataaat ttagaaaatt taataaagag
    30901 taaatacaat actaaagtgt ttatagaaaa tgatacaaat attactacta ttgggattta
    30961 tcaaaaacag tataaagaca aatataaaaa tatggctctt atgtatttag aagatggtat
    31021 tggttcaggt atagttataa atggtgaatt gtatttaggt tctacaaatt ttgctggaga
    31081 gttaagttat ctaaatatag aatttggtaa gtataactct aacaacttaa atcttgaaga
    31141 ttatattatg agtttaagaa aaagatattt agaaagtaaa gataataaat ttaagaaaga
    31201 aattttatct ataatatcaa actttataag gactcttgta tgtgtcctaa atccacaagc
    31261 tatgattata cagtgtagta tattaaccaa gagtgacatg gagtttatag aaaaagatgt
    31321 aagcaataat gttggagaag aaaattttcc taaattaata aaagtagact cattgagaga
    31381 aggaagtata aatggagtaa ttagtatgtg tttaaaagaa actgactatc aatattcctt
    31441 atctaataaa aaaggaggat attaatgtga aatatttggt ttcaatagat ggagggggaa
    31501 caaaaactaa attttgtgta agtgacttag atggaaacat acttaaagaa cataccactg
    31561 gttcaactaa ttataagtca gttggaatta aaaaaacata tgaaaatatt aataatggat
    31621 ttaaaaaaat attaaaagat ttatatatcg attatgacga tattgagtac actgtatttg
    31681 gtatatctgg ttgtgattct cctaatgatt ataagattat aatggatgaa atattaaaaa
    31741 ttgggattaa caaagaaaaa atatatctgg caaatgatgc agttttagct ttttatgcac
    31801 aagctgattc ccctggttta gtaattgtgg ctgggacagg ttcaattata ctgggaatta
    31861 aagaagatgg agaaatatat agagttggtg gatgggggta taattttagt gatttgggtt
    31921 ctggatatga tatagggaga aaattattaa agaaagtatt gctttattgt gatgaatgtc
    31981 atgagtatag tgatttattt agttgtgtct tggatttctt tggagcgaat agttttgaac
    32041 aacttgcata tatgattact gatataaacg ataatgttga aatagcaaac ctagcttcat
    32101 tagtgattga ttgtgccgaa caaggtgata aattggctat tgaaatatta agagaatcat
    32161 caactgaact atctaaatta gctcaagtaa tattgagaaa aatatctaat ccaatgaaaa
    32221 tggataaaag cgaaataaat atagttttgt ctggtggtac attaaataaa actgtatatg
    32281 ctcaaatgtt aatgaataat ttaaaaacag aaaatacaga taaaaattta aaatttatat
    32341 ctcaagaaaa tcagccagta tatgggggga taagacttgc tatggcatta gctaatagga
    32401 gggtcaagca tggatagaaa aaaggtaata gtacaaattt ttactggagg ttttaaggct
    32461 aaagaaataa agtttaatga tataaaatgt aaactactag aattattgaa ctgtattgat
    32521 atagaaaagg ttatcatggg atggagtgta gatgaagaat tatatgtaga aactaaaaaa
    32581 ctattaaata aatataatgt tgaactatat ctttggttgc catgtttttc tgaaatagga
    32641 ttacttgaaa aaagtaattt gctaattgat tatatgggga aggaagtcaa gggctatgct
    32701 ttacaagagg atgaaaattt tgagttttat tgcccaaata ataaggaaaa tataaaaaat
    32761 ataaagaaaa tatatagtac ttattttagt gatattaaat ttgatggaat ttttttagat
    32821 aaaataaggt atggttcttt ttcgaataaa ctaaatggtg tttttaattg tttttgtaaa
    32881 gattgtttaa atatatatga aaaaaataat attgataaag agtcgttaat gagagaaatg
    32941 aataatgtgt ctaagggagt tatagataat gaagatactc catttggtat aacagaatac
    33001 attaatggaa aatacaaatt taaaaataaa atatgggaag atttttttga aacaaaaaat
    33061 caaaatatat acaaggcatt attagagata agtaattatt ttaaaagtaa gggattgaaa
    33121 ataggtatag atactttttc tccttttatt tcttacttta caggtcaaga tttagaaaaa
    33181 ttacaagaag tagcagattt tataaaacca atgatgtata gaactacaaa agctccagct
    33241 ggtttaccat ttgaatttga aaggtttatt gatgaatcca tacataagaa taaagagatt
    33301 ggaaaaagaa atttcttaga tattttagga attaaggagt atgaaggaga tgtatatcct
    33361 atagaatttg tactagagga aatcgatttt atgacaagaa atcttaataa agatatatat
    33421 gtaggagttg aaataaataa aaaggaaatt atatctcctg ttacacctaa gtacataagt
    33481 gaaaatattt caaatataaa taagacaaca gtaaaaggat atgttttatc ttgggatttg
    33541 ttgtcagcac caaaagaaaa tattgatgaa attataaaat ggtttcggaa ttagtgaggt
    33601 gataaagtga gtaagatatt ggaaatgcaa caaatatata aaagttttgg aaaggtagat
    33661 gttttaaaag ggataaattt tgagttagaa aagggagaag taagagctct tttgggagct
    33721 aatggagcag gaaaatctac acttattaaa attttaggag gagttcattc tcaaactaaa
    33781 ggtaaagtaa ttttgactgg taatgaaata atgtttaaaa atccacatca ttctaaagaa
    33841 aatggtatta gtataattta tcaagaatta agcttgatac caacgcttac agtacttgaa
    33901 aatatttttc ttggtagaga aattttaaaa ggaagtgtgt ttttaaacaa gaaagacatg
    33961 cttgaggaat atatgaaaat ttgtgaggat tttgatttta atatagaccc acatatggta
    34021 gtttctaatt taagtgtagc aaaccaacaa atggttgaga taatgaaagc tgtttcatgt
    34081 aattctgaaa ttatcataat ggatgaacct acaacatcac ttactaataa tgaaaaagat
    34141 gggctattta aaataataag tagactaaag tctaaaggta aaacaatagt ctatatttct
    34201 cacatattag aggaagtttt taaagtatgt gatagtacaa gtataatgaa aaatggaatt
    34261 atgattggaa cttatgatgt agaggattta actaaattaa agataactca acttatgaca
    34321 ggaaatgata atcacaaact tattgagaag aaggattata catttgcaaa atatgaagag
    34381 ccaccagtac tagaagtaaa gaatataagc tggtctaaca aaataaaaaa tgtttcattt
    34441 aaagtttata gaggcgaagt tgtaggtctt gcaggtcttg taggttcaag aagaactgaa
    34501 attataaatt tgatatttgg tatagataat aaagatagtg gagacatttt tatcaatggt
    34561 aaaaaagtag acataacttc tcctaaggtt gctataaaaa acaaaatagg attgattcct
    34621 gaagatagaa aaaatctagg attaatttta gaacaagcta tatacacaaa ctcatcatca
    34681 gtacaaattg ataaattcaa gaaattcttg tttatagata gaaataaaga aattgagttt
    34741 tctaaaaaag gtgttaagga attaggaata aaagttagtg atgtaaagca agaagtaaaa
    34801 gagcttagtg gtggaaatca acaaaaagta gtagtttcta agtggttaga gaaagattta
    34861 gatttaataa tatatgatga gccaacaaag ggaatagata ttgctgcaaa agaagatatt
    34921 ttcaatacaa tcaaagaatt ttcaagtaaa ggaataggag taatatttat atcttctgat
    34981 ttagaagaag taataagagt aagtgataga attttagtaa ctagagaagg ttctattgta
    35041 gatgatttaa aaaatgagaa tatacaggtt caagatataa tgaacagtat atttaatgta
    35101 taaaaaatga aaatggagga ctgagatgaa gaaagctttt gagaagttaa atttaagaga
    35161 ttatggagta ttattaggtt ttttagcttt atgtatagct ataagtattg caactccatc
    35221 atttttaggt aaacaaaata tacttaatct tttacgacaa tcttctataa ttggaataat
    35281 ttcagcaggt atgacttttg ttataatatc tggaaacttt gatatatcag ttggagcagt
    35341 agcagctctt gcaggtgcta taacaatgaa atttgcgact ggtggaacta atttgtttgc
    35401 atccatgttc ttaggtgtag cgatttgtgc agtaatagga ttaatcaatg gtatagtagt
    35461 tgcaaagata aatgtaccat cattaatagc cactatggct atggtcacta ttgtaagagg
    35521 attgctgttg atgcttacag gaggatatcc aattactgaa aatataccaa tacttgatta
    35581 cattggaaat ggatatttgt ttaatatacc aataccagta gtaattttct ttatagtggt
    35641 gttagtttcg tttattgtac ttaataaaac taagtttggt agatatgttt attctgtagg
    35701 agggaatcaa gaagcaagta aacttaatgg tataaatgta gattctcaca aagtgaaagt
    35761 gtttattatt aatgctgtac ttgcaggaat agctggaatc gtactaacag gaagacttgg
    35821 gactgcaact gcgattgcag gtgaaggata tgatatggat gcaattgcat ctgtagtaat
    35881 aggaggtaca tcagtggcag gaggttctgg ttctgtactt aaaactgtta taggagtatt
    35941 acttatgagt gttataaaca acagttttaa tcttcttgga atagatgtat atttccagta
    36001 catatttaaa ggattaataa ttttagcagc agttggattt gattcttata gcaagaaaaa
    36061 acttgcttca agataaaaaa taagggggat ttttttatga aaaaattaat gaaacattta
    36121 gcattgttgc tatcacttgt tatgatattt ggtttagttg gttgttcaaa tggtggtgac
    36181 aaagaaaaaa atggagaaaa gaaaatagca gtattactac cgggttcaac aggttacttt
    36241 gtagcaacta aacaaggtat agatgcaaaa tctaaagaat taggtgtaag cgtggaatat
    36301 gcagatgctc aatgggatgc tagtaaacaa ttatcacaag ctgaagattt tatggctaaa
    36361 ggggtagaca tgattgcatt atgtggagtt gattctgctg taagtgagag aatagttaaa
    36421 gctgctaatg attcagacgt tcctatagta gcatttacaa attctatagg aagtaaccca
    36481 actggagaat ttaaaggttt agttacatat ataggacaaa atgaggaaga aacaggagca
    36541 ttaactggaa aaattgctaa aaacttatta ggagaaactg gtgggaaagc tgtattaata
    36601 gaaggagtac caggaactac tccacaagta ttaagaaaaa aaggtcttga aaaagaatta
    36661 aaagatagta atatagaaat agtttataat caaacttcaa attgggagaa agagcaagct
    36721 ttaaaagtaa cagaagattt aattcaaaag aaaactgatt ttaatgttat aatatgtcaa
    36781 gatgataatt ctgctacagg tgcaggtcaa gctttaaaag atgctggtct taaggataaa
    36841 gttaaagtca taggtttggg tggaagtaaa gatggattaa aagctataac tgatgggtta
    36901 atagatggta caacttatat gtctgctgta gaagaaggtg gattagtcat agaaaaggca
    36961 tctaaattcc ttaaaggtga aaaaatagaa cctgttacac aaattaaaca agttgaagtt
    37021 aacaaagata atatttcaga gttcaagggt gaatggtaaa ttgtcataga taataaataa
    37081 gatttttaga gttactttag aaactatata aagtaactct ttttttaatt taatttagtg
    37141 ataaattatt gaatagtatt tctaaacaaa taaaaaatta atataaacaa agacttaatt
    37201 atactaaatt ataagtaaaa ctaatataaa cttatggaaa aaaggaaaac gtctaaagta
    37261 ttggaattac tatagtgtag gtgtggtatt aaaaaaacat aaaaaaacat aaaaaaattg
    37321 ttgaaaaaaa tatataaatt ttgtataatt aaagatttga aaagtgcggt atacaaagcc
    37381 aattcttttg tatataatat atataaattc agaaaaagga agggatatgt atggccaggc
    37441 ggttattttt agaaaggatt ggacttatat ttttaggttc agcaatatta gcatttggag
    37501 tttataattt ttattattta aacaacataa cagaaggtgg agtgttaggt attttactac
    37561 tgttaaaaaa tttattcaat atacaaccag caatagcaaa tgttgtaata gatggtttac
    37621 tacttttagt aggatataaa tttttcggta aaaaattcct tatatattca atagtagctt
    37681 ctataacttt ttcagtgtta tatgacttat ttgaagctat aggacctcta gtaccacaat
    37741 cacaaaatat gcttttatca actattttgg caggtgtaac tgttggaact ggtgtaggta
    37801 tagtagtaaa agcaggttgt gcatcaggag gagacgatgc tttagcatta gtaatttcta
    37861 agactacatc tttgaacata gggcaagttt atttagcaac agatgttatt gtattgttgt
    37921 tatcattatt ttatctatca gcgtttgata tcttttattc tttgattgct gttacaatta
    37981 gtggcaaggt aattgatttt atttattatc atggtaaaag tttggatatg gatataagta
    38041 atgatatagt tccagaatgt taatataaat taaataactt tagattttat taggggtggc
    38101 agatttgcca ccttttttgt gtctaaatct ttatagaaag tataatccca tctaggtttt
    38161 tgtaaatctc aaatatagaa ttatgttgga atgactggga atacgattgc tagatgtgca
    38221 ttttaaaaac caaattttat atttatgaaa tactaatcat taatggatgt ttcttaatcc
    38281 tatctttata cctaaaatga taagataaat gaagtagcaa tacaaaacta aaatttgtaa
    38341 aagagattaa attaaatgga ggagctaaaa tgttggatat agttatgata gcattgttat
    38401 tcataggatt tgtttccatg aagttacttg ctgattggtg tgggaaacaa actgaaaaaa
    38461 aataatgtaa tgtatggagg gattacctat gtggtttctg gcagttgtaa ttataatgtt
    38521 aataatatat ttggtatatg cgcttattaa tcctgaaaaa ttttaaacag tataaggcta
    38581 ataagtaagt tgaattgggg ggaacaattt ttatggtgca gatagtaata gtgttagcga
    38641 tttttatgat tttagtaata cctatgggca agtatttata tcatatagct actaatcaaa
    38701 aaacatttgg agatagacta tttgataaag tggacaattt tatatataag gtatgtagta
    38761 tagataagaa aaaagaaatg aactggaaac agtatgcatt agctttatta tttacaaatg
    38821 cagttatggt atttatagga tatatcatac ttagaactca aagtatgcat atttttaatc
    38881 caagtggaat taaaagtatg gaacaaggac tatcatttaa tacaataata agttttatga
    38941 caaatacaaa tcttcaacat tattcaggtg aatcaggact ttcatacttt agtcagatga
    39001 cagtgataat atatatgatg tttacatcag ctgcaacagg atatgcggct gctatggcat
    39061 ttgtaagagg tctagtcggt aaaaagaaaa cattgggaaa tttttatgta gatttaatta
    39121 ggataacaac aagagtactt ttaccaggag ctttaattat tgggcttata ttagttacac
    39181 aaggagtacc acaaactttt gctgggacag aaacagtgac tactatagaa ggaaagcttc
    39241 aagatatagc tagaggtcct gtggcagctc ttgaatcaat aaaacattta ggaacaaatg
    39301 gtggtggatt ctttggttca aattcatcac acccatttga aaatccaaca ataatatcaa
    39361 atatagtaga aatattatct atgatgatat taccaggagc ttgtgttgtt gcatttggtc
    39421 acatgataaa gaataaaaaa caaggttggg ttgtgtttgg cgcaatgtca atcatatttt
    39481 taataggtct agtagtgtgc tttaaagcag aaagtgcagg aaaccctatt ttatcacaat
    39541 tagggcttaa tcaaagtatg ggaagtatgg aaggtaagga agttagattt ggtatagcac
    39601 aatcatcatt attcacaaca gtgactactt cctttacgac aggtacagtt aataatatgc
    39661 atgatacact gactccttta ggaggattag taccactttt aaatatgatg ctaaatgtag
    39721 tatttggtgg taaaggcgta ggtctgatga atatgttgat gtacgctata atagcagtat
    39781 tcttatgtgg attaatggta ggaagaacac cagagttttt aactaagaaa atcgaaggaa
    39841 aagaaatgaa gcttattgct ttacttatta tattacatcc acttttaata ctaatgttct
    39901 ctggtctttc agtagcaata ccagcaggac ttgaaggaat ttcaaatcct ggattccatg
    39961 gattatcaca ggtattgtat gagtttgcat cttcagcagc taataatggt tcaggatttg
    40021 aaggtttagg agataacact atgttttgga atataacaac aggtatagtt atgttctttg
    40081 gaagatatgt gtctattata gtactactag caatatcaag tttgttagca tctaaaaaag
    40141 ctgtaaatga aagtatagga acacttagaa cggataattt tacatttaca atagttctag
    40201 tacttgttgt tttaattgtt ggtgctttaa catttttccc agccttagcg cttggaccta
    40261 tttcggagca tctagtgttg tggcactaaa aaattgggag ggagtaaagt tttatggaaa
    40321 ctaaaaaaac aaagtttatt acaaaagata ttttcaaaac ttccattata gaagctttta
    40381 aaaaattaaa tcctaaatat atgatgaaaa atccagttat gtttgttgta gaaattggat
    40441 ttatagtaac attgatactt acaatagtac ctagtttgtt tggtgatgaa ggcaataact
    40501 taagagctta taatgggatt gtaacaataa tcttatttat aacagtcctt tttgcaaatt
    40561 ttgctgaagc tgtagctgag ggaagaggta aagctcaagc agatacactt aagaaaacta
    40621 gaaaagatac agtagcaagg gttatagata cgttaggaaa tgaaaaaatg ataagtgcaa
    40681 gtgagctaaa acttggtgat attgttttag taaatgctgg agaagttatt ccaaatgatg
    40741 gagaagttat agagggcata gcttctgtag atgagtcagc aataactgga gaatctgcac
    40801 ctgtcatgag agaatcaggt ggagatttcg catctgttac aggtggtact acagttgtaa
    40861 gtgactggtt aaagataaga ataacaacaa aaccaggaga atcgttctta gataaaatga
    40921 tagctcttgt agaaggtgct tcaagacaaa aaacgccaaa tgagattgct ttaaatactt
    40981 tgttagtagg gttaacactt atatttatgg ttgttttaat atctctttat ccaatggcaa
    41041 actatgcagg tgtaaagatt ccaatttcta ctatgatagc tcttttggta tgtttaatac
    41101 caactactat aggtggattg ttgtcagcta ttggtattgc aggtatggat agagtaacta
    41161 gatttaatgt aattgcaatg tcaggaaaag cagtagaagc ttgtggagac gttgatacta
    41221 tgattttgga taagacaggt acgattactt atgggaatag acttgcatca gaatttatag
    41281 cagttggaaa tgcagattca aaagatttaa taaattattc tgttatgtgt tcattaaaag
    41341 atgatacacc agaaggaaaa tctatagttg atttaggtat gaaattaggt tcaacaggta
    41401 agacaaaaga ggcagaggaa gctgagtttg tagaattttc tgcacaaact agaatgagtg
    41461 gtattaactt agctgatggt agagctataa gaaaaggtgc ttatgatagc ataataaaaa
    41521 gagtaaaaga atctggtggt acagtgccag aagatttaga agaaaatgta aatcgagtag
    41581 ctaaactagg aggaacacct ttagtagtgt gtgtagataa tgagatatat ggaattatct
    41641 acttaaaaga tacagtaaaa tcagggcttg tagaaagatt tgctagactt cgagagatag
    41701 gaataaagac aataatgtgt acaggggata atcccttgac agcagctaca attgcaagag
    41761 aagctggagt agatggattt attgcagaat gtaaaccaga agataaaata gatgcaataa
    41821 aaaaagaaca attagagggt aaaatagttg caatgacagg tgatggaaca aatgatgctc
    41881 ctgcacttgc tcaggcagat gtaggtcttg caatgaatag tggtacaaca tctgcaaaag
    41941 aagcagcaaa tatggtagat ttagattcag acccaactaa gatattggaa gttgttgaaa
    42001 taggaaaaca gcttttaata actcgtgggg ctttgacaac atttagtata gcaaatgatg
    42061 ttgcaaaata ttttgcgatt ataccagcta tatttactct tgcaatacct caaatggaga
    42121 tattaaatgt aatgaaactt tctacgtcat ttagtgcgat attatcagct ttaatattta
    42181 atgctataat tatcccatgt ttaatacctc ttgcaatgaa gggagtaaaa tatagaccta
    42241 tgaaatcaca agctatgtta atgagaaata tgcttatatt tggtcttggt ggagttatag
    42301 taccattttt aggaataaaa ttaattgatt tattgattac acctttagta atggttctta
    42361 atttaggtta ataaatggag gatataaaaa tgaaaacatt aaagccagca ttacttgtaa
    42421 gtattgtatt gttagttgtt tgtggacttg tatatccact tgttttgaca ggtgtaagtc
    42481 aagttgcatt taaagataaa gccaatggaa gtatgataga agtaaatggt gtaaaagtag
    42541 gttcagaact tattggtcaa agctttacag atgcgagatt ctttaaggga agagtatcat
    42601 cagtaaacta taatacttat acaaaagaag atttagtacc agataaagat ggaaatacat
    42661 cttatggagg tgtatcatca gggtctttta attatggagc tacaaatcca gaacttcatg
    42721 atagagtaca aaaagatata gaaaagtttt taaaggataa tccaactgtc aaaagagagg
    42781 atataccaac agatttactt acagcatcag gttcaggact tgaccctaat ataagtccag
    42841 cctctgcaaa aattcagata ccagctatag caaaagcgtc tggaatatca gaaagtaaat
    42901 tacaaaaaat tgtagatgat aatacttcca aaaaactttt tggagtttta ggtgaagata
    42961 gagttaatgt attaaaagtt aatgtagagg tcgcaaaaat attaggtctt atatagtatt
    43021 ttgtggaatt ttagcaacaa aatattactc aagatttaat ttaaaggtta aattttaaag
    43081 agtgaaaatc gataatgatt ttcacttttt tatttgaata ttactaatat ttggaataaa
    43141 gtgattgtaa gtataaaatc caaaaaaagg ttgcattatc aaaatgataa tactataata
    43201 tatttatcgt tatcaatttg ataatagatt tggacatata ggaggaaata gtatgttata
    43261 taataacgca ttagagttga taggaaacac acctgtagta aggctgaata atttaggctg
    43321 tactaatggg tgttctaaca tatatgtaaa attagaaaaa gtaaatccag caggaagtat
    43381 aaaagataga gctgtttatg aaatgttaga gggcttagag caaagaggag aattaaaaaa
    43441 gggagatgta ctagttgaag caactagtgg aaatactggt attgcattat ctatgatagg
    43501 aaaattaaaa ggatatgaag tagtaatcgt aatgcccgaa acaatgagcg ttgagagaag
    43561 agatttaatg aaggcttatg gagcaaattt ggtgcttaca gatggtaaag ctggaatggc
    43621 aggttccata gaaaaagcaa atgaactttt aaaagaaaat ccaaactata aaagcttaaa
    43681 acaatttgaa aataaagata atccaaatgc acattataaa acaacagcag ttgaaattat
    43741 gaatgatgtc aaagatttag atatattcgt atgtggagtt ggaacaggtg gtactttaat
    43801 aggaactgca agatatttaa aagaacaaaa tccaaatata agagtaattg cacttgaacc
    43861 aaaaaaatct ccagctatat cagaaaacaa gactggacct cataaaatac aaggaatagg
    43921 tgctaacttt gttcctgaaa attatgattc tagtgtagtt gatgaagtaa tattagtaga
    43981 tgatgaagat gcatttgata cagttaaatt acttgcagca aaagagggaa ttttagtagg
    44041 gatatcttct ggagcaaata tatttggtgc tatagagatg gcaaaaaagt atccagataa
    44101 aaagatagtt acagtagctc cagatggagt tgataagtat atgtctatgg gaatatttta
    44161 ataattttat ttgagggagt gaatactttg tttaagaaaa taaataaaga tattgaatat
    44221 ataatgaaga atgatccagc agctaggtct aagatagaag tttttttact ttatccatca
    44281 gttcatgcaa tgataatgca taggatggct catgctcttt ataagaagaa gaagcttttt
    44341 acagcaagat taatatccca gatatccaga tttatgactg gcatagaaat acatcctggt
    44401 gctaaaatgg gggaaggtat attgatagac catggaatgg gtgttgtaat tggagagaca
    44461 gcggaagtag gtaatagggt gacaatatat caaggagcta ctttaggagc tacaggaaaa
    44521 gatactggaa agagacatcc aactgttggt gatgatgtat taattggtgc tggaacaaaa
    44581 atactaggac ctttaaatat aggttcaaat tcaaaaattg gtgcaaattc tgttgttgtt
    44641 aaggatgtac ctaatggtgc aacagttgtt ggtataccag caaaaatagt aaaaataaga
    44701 aacctagaac ctgtaaaaaa gaataaaaaa gaagtgtcct atgagtatga tgaattagat
    44761 aatgtatatt atatatagga tttatataac tataaaataa actttataaa aattatttta
    44821 aaaggtattt ttttcaaatt tgaggacata ataaaaaaga actgaatgtt acagttttgg
    44881 ttcatcaatc tttgataaat aagattttaa atttgtagcg tctaggagga ttttattatg
    44941 aaagcaaatg ttaatcaaga tacttgtata ggttgtggat tatgtccatc aatatgtcca
    45001 gaagtatttg atatgaagga tgatgggaaa tcacatgtta tagttgatga agtaccaagt
    45061 gatgctgaag aatcagcagc agaagctaga gaaagctgtc cagttgatgc aatagatgtg
    45121 cattaataaa ataaatgcct ataaaaattg aattttcaat ttttataggc atttgtcttt
    45181 tattcagtta gtatttcaac acctgtttct gtaactaaaa ccatacattc ccattgagca
    45241 gataatttac catcttttgt ataaatagtc caattatttt cttcatcaac aaaaatttcg
    45301 tgagttcctt catttaccat aggttctatt gtgaatatca tcccaggaac caatagcatc
    45361 tcagtccctt ttttagatac ataacttaca aaagggtctt catgaaagct taatccaact
    45421 ccatgaccac ctatttctct aacaacagag tatccatttt tctttgcata agagttgata
    45481 gcatcaccta tatctcctaa aaatccccat ggtttggcaa cttcaagacc tttttggaca
    45541 cattcctttg taacttgaac taattttttt gcattttcat taacattacc tatcataaac
    45601 attcttgaag catctgaata gtatccatta aaaatagtag aaacatctac atttataata
    45661 tctccatctt ttaagataat gtccttactt ggaataccat gacaaacttc attatttact
    45721 gatgtacata cacttttagg aaatccatta tagtctagtg gagcagggat agctccattt
    45781 tgtagtgtaa agtcatgaac caacttattt atatcttctg tagacattcc aatttcgatt
    45841 ttatctgaaa tataatccaa aatttttgtg tttattacag cacttttttt tatttcctca
    45901 atttgacttt cattttttat aatatcatga gttggtacaa tatgacctaa cttagcaaat
    45961 gaatctattt tattatccat atccatatga cattttttat attttttagt acttccacac
    46021 caacagctat catttctatt taacttcata acttttcatc cttcttttat tatttattta
    46081 aattatgggt tacataaact attttaaaac ataaccatta taaatattat aacataaata
    46141 ctaagaaatc ctttatagat agtgatttat tcaaagtata aattattata tggttattgt
    46201 tgatgaaaag aggatagtta aatgtattat tggatattag cgcacttaat agtttagttc
    46261 aagtttttca agaaaatcat atgttagagg acaattttcc tttaaagtat tttttgaatc
    46321 ttcatcaaga taataatgta caaatgattc tgcaaaaaat tcttctataa acttgcttat
    46381 atatgcatgt gcaacttgct tagggtcata aagcttatat ctttcctgag caaatacttc
    46441 cataaattct tcagaatttg attttttatt taaaacattt ttatctatag catgtgcagt
    46501 ttcatgtaat tctaaatttt ttgatgaatg tacatttgca tagctaggat tactataacc
    46561 tattttagcc actatagaat tatttctata tataccaggt acatctttcc aagtcttgcc
    46621 tgatcttacc caacatggag gtaattgata ttttaaatca ctaaattctg gttcatcagt
    46681 tagatttgaa tttattaatt ttatttttat atccttttct ttaagtgtct gtaaatattt
    46741 aatatttatt tttgcaagtc tgcttttcat attttcaact tcatctttat cataattcaa
    46801 gtcatcagga agtataagca tttgttcaat tatatctata ctactgtctt ttatattttc
    46861 agattcttca tcaaatttat attgaagagt attattttca tagatagtta tatttgtatt
    46921 attttctata gaataagagt gaatatcctc taaatctaaa atattcattt gttgtaaaat
    46981 atgttttgtt tcgccactcc atactactaa agaaagtgaa ttaaaattaa ttagtggatt
    47041 ttcttttgct tttatatcat tgcttatatc tacatcaaat acatattcaa ttgagtcttc
    47101 tatgttaaga ctatctgtat tctttttaat atataaatac gcttgtttta agtctatata
    47161 aatctcacct aaagattcgt cataaaattc tgataaatta ttagtaaaga gttcttcact
    47221 taaaagttca tttttatcag ttatactttc aattaaaata ttaatttctt ttatcatatc
    47281 gtaaatactt atttttctat ttgctttttc actagataca ttgcatggcg caagagcaat
    47341 gcaataaaaa tctttttctt ttttataaaa ggctttaagt gattttgata aaagtgtaaa
    47401 gtctacacct atctgtaagt tgaccttaag tgagtcattt tgtaataatt cagtattatt
    47461 tcttttagct gtatttaaat tgttttccat aatcattgcc ccctttttat tagggtatca
    47521 taattttttt atatgtgtca atttgttttt ttgaaaatta taattttttt gaaaatttta
    47581 ttttttataa tttgtgaatt ttttattata gaaataaata tgttattatt atgtcaaaat
    47641 ttagcattag attcatgtag agtatgtttt atacatatat tataattaat tctttgaaga
    47701 ttgcatattc aaaatacaca ccattttttt taattctata atataatatt tatatattta
    47761 atgttaggat gtgatttaag gtgtatttta catatattat aagatgtaaa gatgattctc
    47821 tatacactgg atataccagt aatatagtta gaagaatgaa tgaacataag ttaggaatta
    47881 attcaaaata cacaagagca aagggatttg aaaaactaga agtttatttt gtgaccaaca
    47941 caaaaagcaa tgctatgaag ttagaatact atataaaaaa attaactaga aataaaaaat
    48001 tgtctataat aaaaaaccca agtatactta taaatttaat tgataataaa gaagattata
    48061 ttattggtaa gaaaatagaa caattaacat aaatatttat agtaacatta taatgggtgc
    48121 atatggtgta aaaacttgac aataaatact attgtaatat aatgaagtta taaatatagt
    48181 aaattaaaat agctagggga gccaagagct gagaggtata ccgaccctta cacctgatct
    48241 ggataatacc agcgtaggaa agcttagtaa aaattcaagt atgtattatt tgtgatatat
    48301 gctatttcta agctatacct attgtaggta tagctttttt aattaagtta tttaaattaa
    48361 actttaggag gcaacaatga gtaattataa aataccaaca cttacaatag ccggctctga
    48421 ttcatctgga ggagctggga ttcaggctga tttgaaaacg tttagtgcaa ttggtactta
    48481 tggtatgagt gtaataacag ctataacagc acagaatact aaaggagttt ttgctgtaga
    48541 ggatttaaat aagaaaataa taaaaaaaca aatagaggca gtctttgaag atatacctcc
    48601 aagagctgta aaaataggaa tggtttcaag tcctgagatt atacttgaaa tagttgagaa
    48661 tctaaagaaa tacaatccaa aatatttggt tgtggaccca gtaatgatat ctaaaagtgg
    48721 atattatcta ttaaagccag aggcaaaaga aaacttaatt aagtatttga taccacttgc
    48781 atacataatt acaccaaata taccagaggc agaagaaata acaggaataa aaatacataa
    48841 tgtagatgat atgaagagag taggagaaga aatactacaa cttggaccta aatttgtact
    48901 tatgaaagga ggtcatttag atggagaagc agtggatatt ttagttggta aaaatatatt
    48961 taaagtttat aaaagtgaaa gaattgataa aaaaaataca catggaacag ggtgcacatt
    49021 atcttctgca ataacatctt atctagcatt aggatatgaa ataacagaag cagtaaatct
    49081 aagtaaaatc tatataacag aagcaataaa aagaagtttt gatataggtc atggagtagg
    49141 accagtacat catttttata aatttgagta ataaaaaaat attggaggta aaatatgtat
    49201 aatttaatta aagatgtaaa aaaattaaat ccattggtaa ttcattatac taataatgta
    49261 actataaatg attgtgctaa tgtaacttta gctgtaggag caagtcctct tatgagtttt
    49321 tcatatgagg aagtagaaga aatggtaagt gtagcaaact ctgtagtaat aaatatagga
    49381 actatgaact caaatatgtt ggatttattc ctattagctg gaaaagcagc aaataagtac
    49441 aataaacctg ttgttttgga cccagttgga gtatttgcaa gcaaagcgag agcagaactt
    49501 acaagtagac ttttaaatga agtcaaattt agtgttgtta aagggaatgt atcagaaata
    49561 aagtttatag gtggctttaa tgtaaagggt aaaggtgtag attcatttga tgaagaagaa
    49621 gattctactg agataataag aaagattgct gaaaagcttg aatgtgtagt tgtagcaact
    49681 ggaaaaatag atataataac taatggtaaa ggtacatata aaataaacaa tggaactgat
    49741 aaattaaaag gaattacagg aactggatgt atgactgcta gcttgattgc aagttttatg
    49801 gctgttactg agaatatatt agaagcagca acaatgggtg ttcttacaat gtctttaagt
    49861 ggggaattag ctaatttaaa taatccacca ataggtacat ttaaagaaaa tcttatgaat
    49921 gctatttatc aaatggatat agatacttta agtaaaaatt ctaatattga atttttaaat
    49981 taataaagag taagggagac ttaaaatgat tgataaagaa tctttaaaaa aatgtttaaa
    50041 gttgtattta gtaactgact ctgaaatgct taaaggtaga gacttttata aatgtcttga
    50101 agatgcaata tcttctggaa taacaactgt acaacttaga gaaaaaaatg cttctggaag
    50161 agagttttta agaaaagcta tgaaacttag agaaataact aaaagatatg gtgttaagtt
    50221 tataataaat gatagagtgg atatagcact tatttgtgat gcagatggag tacatgtagg
    50281 acagagtgac attgatgtta gagaggttag aaagcttata ggaaacaata aaattctagg
    50341 agtatcagca agaactttag aagaagctat ttgtgcaaaa aatgatggcg cagactattt
    50401 aggtgtaggg tctatattca caacatctac taaattagat gcaaaatcag cttcgtttga
    50461 aactgttaaa gaaattaaag aaaaagtaga catgccattt gtactaattg gaggtataaa
    50521 tttagacaat atagataaac ttaaatgttt agaaagtgac gggtatgcta taatatcagc
    50581 tatattaaaa gctgaagata tctctaaaga agtggaaaag tggacattaa aaatataaaa
    50641 ttggctctgg tagtgaaaaa aatggaaaaa tttgatataa tctaagagta aaatttaagc
    50701 aattataaat gataaacttt tctacatata ctttttaatc aagatatatg tagatttggt
    50761 taaaaaggag ctataaatga ttaggatatt gttagtgtgt gttggaggca tgtcttctac
    50821 cctattggta aataaaatgg agaaggatgc aaaaaaaaga aatatagact gtaaaatatg
    50881 ggcagttgga gaaggagata taaaatcgga gcttgataac tttgatattt tacttcttgg
    50941 accacaactt agatttatgc ttgatgacgt aaagtctata gttggagata gagctccagt
    51001 atctataata gatatggtaa actatggcac ttgtaatggt catgctgtgt taaattcggt
    51061 tttagaaata ttaaaataaa taataaaagt ggattctata taatagagtc catttttatt
    51121 atttgttatt aataggctta aaacttagct taccatttct ttttttaatc tccacatagc
    51181 aagtgaaaaa cttgaacagc acaatattaa aaatagtagg ataaaaggca gtatatctcc
    51241 aataagaata gtctcaccaa atattaagct acgtaaagcg ttaataacat gggtaaatgg
    51301 attgagcatc actgctactt ttaatcctcc agataaactt tctataggga aaagagctga
    51361 acttaaaaag aaaataggaa gtacaattgc agtcataatt gtttcataaa ctatttcatt
    51421 tggcaataat aaacttatag aataagctag acttgataaa aagaaggctg tcataaatat
    51481 cagtactatc ataagtagga ttccaccaat gccagattct atccttactg agaagaatat
    51541 acttactata cacaaaatag ttacttctat gaaagaaact aagatagctt ctaataattg
    51601 accaaggacg attgaatacc tgctgatagg tgcaattaag actctataga aacttccact
    51661 gttcttcatt ataaagttta taataccacc actactacaa gaagaaaaca caaccagtac
    51721 aatgatacct ggtagtataa aagcagtata attattgata tttatattat tcatagtttg
    51781 gtttgcaata gaactataaa gcacaagcca aagaaaaggt tgtaatatag taactacaaa
    51841 tgagattgga tttttaaaac gccacttcat actacgccat aagatattta acattcccat
    51901 tcctccttta agcttttttt agaacttgta agtgctaaaa atacatcttc gagacttggc
    51961 tctacaattt ctattgcatc aaattctata tcatgttcta aaagccattt atttattaaa
    52021 gtaaaagcag ttctactatc gtttacactt ataaaaattg aattttctcg tacgctcata
    52081 aactttacta aaccagtact tttaattgaa tctttatatt tttttatatc ttcagtatta
    52141 tgaaaagtta tacgtagtat attttgtcta atgtaactac gtaaacttga tggtgttcct
    52201 tgtgctaaat cctttccatt cttcataata caaatattat cactcaattg ttcagcttct
    52261 tcaagataat gagtagttag aaatatagtt gtgccatact catctctaat ttttaataac
    52321 atatcccaca tagattttct tgaatcgaca tccataccaa cagttggttc atctaaaaat
    52381 aggattttag gagatgatac catattcata gctatgtcca atctacgttt tacaccccct
    52441 gaatatgatg aagtaggata ttttaagtaa ctagataagt caaacttatc aattaaagag
    52501 tctattcttt gttttgcaat ctgtggttct actttataca ttttactttg aaataccata
    52561 ttttccataa gggataaatg ttcatcaata gagatttgct gtgctacaca agcaatttga
    52621 gtacgtatcc aagaagaatt atctactaaa tcttttccaa acatttttac atttccagat
    52681 gtaggtttgt aaaaggtagt caaaatattt ataagagaag atttaccagc accattagga
    52741 ccaagtaaag aaaaaatttc tccagcattt acattaaagc ttagaccatt taaagctttc
    52801 acaccatttt tatattcttt tactaaattg tctatttcaa tcgctaacat atattattcc
    52861 tcctctttaa gtggaaatat tatctctgta atatagttat ctgggtttcc ttttatcaac
    52921 atacctggtc ctttaataaa tacttctctc caaggagttt gtatttttat attattttca
    52981 tgaatatatt taaatatcat ttcatacgtt tttggcaaat tactataaga gcctgtgtga
    53041 gtaaaaaata aagcttttgt tttaggaaat tcttttgtag ttatacccat acgatttgga
    53101 gtttcagcag ttggaacaca taattccatt tctgcaacac cagttttttg gtcaacatca
    53161 tagtagcaaa agaatggtgc accgttagac tttcctttta tagctttaaa tacatttgga
    53221 aaatactttg ttgcctcagt cataggtcca ttataacgca tggtagccac tgtgactgat
    53281 tcgatatttt tgatttcaat tttgcaattc atttgtttac ctcctaatgt gtgttcatat
    53341 tcttactaga tttttaagca ttaatacata taagattttg attagttgaa aacatataat
    53401 cttatctcgt ttgcttgttt tcaagtctaa cataacataa aatatatatc ctctcatttg
    53461 atgctccatt ttgtaaagtt attatttaag ctttacttta gattttttag caaattaaaa
    53521 taaagcaatt tataaaataa gtatttacaa attaagtgta ttaatcgtat aatacactta
    53581 ataggatact agataaagtc tgaaataaag gcgtattttt ttaaagtaag tgtactatcg
    53641 tattaataca ctagaatagg aggaatcatg aattttgaat tagataatac aactcccatt
    53701 tatttgcaaa tagtaaagta tataaagaga caaatagtaa caggagagtt gaaaccagga
    53761 gagacaatac catcaagaag agaaatggca cttaatctaa aagtaaactt aaatacagtt
    53821 caaagagcat ataaggagat gggtgatatg aatataataa atacttttaa aaattatcaa
    53881 agcagtgtaa cagttgatga aaatatatta aaaaatttaa aattagagct tataaatgaa
    53941 tcactatctg tttttataga agatatgaaa gcaataaatg ttagcaaaga agaagtttta
    54001 aaaataatag aagataagta ttagtttttt aacaaataga aaacttagtt tagaaagttt
    54061 atttataagc atattggatg tggaggatag taaatatgat agaaataaag aatgtcagca
    54121 aaacatataa aaggatgcaa gggcttaaaa taagaaagat agaagcttta aaaaatgtaa
    54181 gttttaatat agaaaaggga aaaataactg cattacttgg tataaatggt gttggtaaat
    54241 caaccatgtt aaaagcaata gcaggactta taaacattga tagtggagaa attcgtatag
    54301 atggagaaaa aataaatgaa aaagtataca ataaattggc ttttgttcca gatgtacaat
    54361 ctcactttag caatacaaca ataaaagaaa catttgaatt tatggaaatt ttttattcaa
    54421 agtggaataa agaaaaatca aaagaaatga tggacatatt taaattggat gaagatgaaa
    54481 taattgataa cttgtcaaaa ggaaacattg caagagtaaa attaatactt ggattttgcc
    54541 aagacccaga gtacatactt cttgatgaac catttacagg aattgatttg tttaagaggg
    54601 aagagtttat aggagtaata gcgcaatata tggaggaaaa tcaagccatt atcataacta
    54661 ctcatgagat agtagagata gaaagtttag ttgatgaggt tgtaattctt gatgaagggc
    54721 agattataac ttcatttaat gcagaagaat taagagaaag agaaggtaaa tctatattag
    54781 ataagatgag ggaggtatat aagaatgaat aaacttctat atctaataaa tgtagatttg
    54841 agaagaagca ataagtttta tatagcttat atatcatttt tttcattaat tacacttagt
    54901 ttaaatttat tcgaaattaa taaatttaaa aattctaaat ttattatgaa cttagcaata
    54961 gaagattttg gaggtatatt ttatggacta gggatactta gtaatcttgg ttttatccaa
    55021 agtatattgt tctttgggat attgggactt tttatatact atatttttat gtggaaaaga
    55081 gagttctttt caaataatca aagtatttat acacttatga tgttacctca aaataagttt
    55141 aagatttgta tatctaaatc aatagccttg ataacaatga tatatggttt tttgataagt
    55201 cagacagcaa cattatttat atgtaaatat atatttaatt tcatttttag aaatatgcct
    55261 ataatcaata tgaactttgc caaagattta tattatttaa acttgaagag tataccaatt
    55321 gattttatct cttttatggc tatttatgta tttttactac ttatagctat ttctattgta
    55381 aattgctgta tattatttat cttttcctat ataaataaat tttatgtaat tttaatcact
    55441 ttgctgatta ta
//
LOCUS       DBGJFF010000009        51059 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.009, whole genome shotgun sequence.
ACCESSION   DBGJFF010000009 DBGJFF010000000
VERSION     DBGJFF010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 51059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51059
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.009"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(<1..1382)
                     /locus_tag="ACOIAF_000631"
     CDS             complement(<1..1382)
                     /locus_tag="ACOIAF_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HGZ2667658.1"
                     /translation="MYKLIDKKDIDFLIDTCGEENVLVGSDINEDFSHDELGGIEKYP
                     EVLVNVLETEQVSKIMKYAYKNNIPVTPRGQGTGLVGAAVAINGGIMINLCKMNKILE
                     VDYENLTLTVEPGVLLMTIGQYVQDRDLFYPPDPGEKSATIAGNINTNAGGMRAVKYG
                     VTRDYVRGLEVVLPNGEIINVGGKVVKNSSGYSIKDLLVGSEGTLGIVTKAILKLLPL
                     PKKSISLLIPFPDLSMAIETVPKIIKSKSIPTAIEFMERDVILAAEEFLGKKFPDNTS
                     DAYLLLTFDGNSTEDIEKEYEKVANLCLENGALDVFISDTQERNDSIWSARGAFLEAI
                     KASTTQMDECDVVVPRDKIAEFIRYTHELQDKLKIRIKSFGHAGDGNLHIYILKDGMD
                     DNTWKIRLKETFDYMYKKSRELSGQVSGEHGIGYAKKEYLHESNSDAYMMLIKNIKLA
                     FDPKNILNPG"
     gene            complement(1393..2613)
                     /locus_tag="ACOIAF_000632"
     CDS             complement(1393..2613)
                     /locus_tag="ACOIAF_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HGZ2667659.1"
                     /translation="MSKIVVNQDKITDLKRILEICPFGAIEEKSGIVEISAGCKMCKL
                     CVKSGPKGAFEFIESSKVQINKDEWRGIAVYVEHHNGNIHPVTYELIGKAREMASKIK
                     QPVYCVFVGKDIKDKCSNLISYGVDEVFVYDEDEFKDFRIEPYSKAIENFIDKIKPTI
                     VLVGGTTLGRSLAPRLAARFRTGLTADCTILDIQSNTDLDQIRPAFGGNIMAHINTPN
                     NRPQFATVRYKIFSAPEKIENTTGKITLCKVEKKELKSKIKVLSVKEKNKEVGLEEAE
                     VIVVASRAIKKQEDMEMIYKLADKLNAQVAGTRPVIEAGWIDAKKQIGLSGRTVKPKL
                     IITCGVSGAVQFVAGMQGADYIVAINKDDKAPILDVAHLALIGDIYDIIPKLIEKIEN
                     NKDNNQKYMASAAK"
     gene            complement(2627..3415)
                     /locus_tag="ACOIAF_000633"
     CDS             complement(2627..3415)
                     /locus_tag="ACOIAF_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HGZ2667660.1"
                     /translation="MEILTCIKQVPGTTSVEVDETTGVLKRDGVDSKMNPYDLYALET
                     ALRIKEDKKANLKVLSMGPPQAKKVIEESFMMGADEGALISDRRFGGADVLATSYTIS
                     QGIKKMGKVDLIICGKQTTDGDTAQVGPEVAEFLDIPHVTNVTKLIEVKDESIVVEID
                     MPNDLQVCEIEYPCLITVEKDIFQPRLPSFKLKLNTKDREIPVYSLDDFEDKNENNYG
                     LNGSPTQVVRIFPPKPNTDKNIVRGNADELSFALVNKLEELKLV"
     gene            complement(3432..4706)
                     /gene="larA"
                     /locus_tag="ACOIAF_000634"
     CDS             complement(3432..4706)
                     /gene="larA"
                     /locus_tag="ACOIAF_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HGZ2667661.1"
                     /translation="MVKLKVPYSKQGMILEIPDERFLGVLESKSDNYIEHKTQEEIVE
                     ESMNNPIGSNSLEELVKGKKNVVIITSDHTRPVPSRITMPIILRRIRNVNPEIDVKII
                     VATGFHRPSTREELIYKMGEEIVDNEDIVMHISTDDKSMCKVGILPSGGDLYINKLAY
                     EAELLIAEGFIEPHFFAGFSGGRKSVLPGIASAKTIMYNHCSDFIDSDNSRTGKLNNN
                     PIHEDMVYAAKVAKLAFILNVVIDKDKKIIASFAGDVEKAHTKGCRFVTELSKVNSIK
                     SDIVVTTNGGYPLDQNIYQSVKGMTAAEATCKDGGVIIMISACNDGHGGQSFYENVAN
                     ADSPKQLLDKIRSVDRVDTIPDQWEFQILARILSKYTVIMVTDKCNPEMIKKMHMKHA
                     FNFKEALEMANSIVGKESKVVVIPDGVSVIVK"
     gene            complement(4824..5537)
                     /locus_tag="ACOIAF_000635"
     CDS             complement(4824..5537)
                     /locus_tag="ACOIAF_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HGZ2667662.1"
                     /translation="MFNSIASKKVYEQVIEQIQYKILNGELKKGDKLLSERELSEQMN
                     VSRTSIREAIRVLETMGVIESRQGEGNFICTNIEKTLIEPLSMIFKLNNGTLEDILEL
                     RIILEIEIAKLASKRITSSEVIELKHIIDEMRVETNKKDNNRVLVLLDQKFHSKLATL
                     SKNYLIQSLFMTASKLFDGFIEDAREKIIAEPFNENILLKQHEAIYNAVVENDVELAC
                     EKAKEHMDFISKNYRKNEN"
     gene            5662..6921
                     /locus_tag="ACOIAF_000636"
     CDS             5662..6921
                     /locus_tag="ACOIAF_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YlbJ"
                     /protein_id="HGZ2667663.1"
                     /translation="MKKNKIIAFFAPSIIVGVLVLGIILYPNEAIKSAREGFSIWSNV
                     LVPSLLPFIIGANLIVDLKIVDIIGFIINPITRFVFNVSGKSALAFAISTVSGYPVGV
                     SLASEFRSNGQISKYEAQRLVSFCSTSGPLFIIGSVGTGMFQNSNLGYLMILCHYLGT
                     ILVGLFFRNYGNENLPKTKLDLKTNIKNVINIRNSSGNGFFVLFGNAVFNGVNTLLTV
                     GGFVIVFSVVFKILSLFNVISLIASVIYLPLSFLGVSKELCQAFVSGLFEITIGCNKV
                     SSITSSPEILRASLASFLIGFSGLSILAQCCTFLSKTDIDLKLYILSKFAHGVLAAIF
                     IFILYPIANSAVLVSSFADTYNVIYNNLIWFYYLSYYDTILQIVVIIYLISAVFIAKK
                     HMKTLSTNGKLMRYKNTFFTKKFSRKK"
     gene            7201..7767
                     /locus_tag="ACOIAF_000637"
     CDS             7201..7767
                     /locus_tag="ACOIAF_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGZ2667664.1"
                     /translation="MATRPIEIDEYKKIMELLHTGFTYSENGVEKRFRKNPKVALALM
                     LEANLGLRISDILRLKIGNFKGNMLEINEKKTGKLQYRPVNKNIIESINEHARKYNLK
                     SNDYLVNIKTKAIQKQLRIICKHLNLYNISTHSFRKLYATTQFEKSNNNLELVKELLN
                     HSSVATTQRYIRVTQQAINEASENFFIG"
     gene            complement(7968..9173)
                     /locus_tag="ACOIAF_000638"
     CDS             complement(7968..9173)
                     /locus_tag="ACOIAF_000638"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HGZ2667665.1"
                     /translation="MKIAILGYGTVGSGLIDLIENNTSKRDIEVVGILVKNKEKHKYK
                     KYFDKITTDIEDIFDKDIDILVEVIGGLNPAFDYVTRALNKKIHVVTANKDLLAEKGS
                     DLIELANLNNVSIKFEASVAGGIPVLKPLIESLEGNNIKSINAILNGTCNFILSKMYD
                     ENLPYDVALKQAQELGFAEVNPDADVLGYDSARKLSILSTLSYGKIVYWKDLLLEGIT
                     SIDEKDIEYAKKLNCKIKLVARSKYESGEVSGFVRPALVDNNNMLSKIDNEFNVVILV
                     GDSVGELSFVGKGAGRGATGSAVYADIIDIIDNRSSNIKSFSKGKLDLSGLIEDECSA
                     VVRFGGYNNKDGILECLDKYVDSYDIIDDEELAIFVSAKSEHEIDRVLEIVKRNNYSE
                     SAKKLLKID"
     regulatory      complement(9276..9382)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(9474..9842)
                     /locus_tag="ACOIAF_000639"
     CDS             complement(9474..9842)
                     /locus_tag="ACOIAF_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HGZ2667666.1"
                     /translation="MLKGKQRAYLRSLANTLKPTTQIGKEGVTESFLEQLDGMLRTRE
                     LVKVTILENAGLDTKETANAVCEALRAEFVQAIGFKFTIYKRNIEEPKILFPGHEQAK
                     AKTKNNNVTKKGKPTKRAVR"
     gene            complement(9894..11171)
                     /gene="obgE"
                     /locus_tag="ACOIAF_000640"
     CDS             complement(9894..11171)
                     /gene="obgE"
                     /locus_tag="ACOIAF_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HGZ2667667.1"
                     /translation="MFIDKARIFVKAGNGGNGSVAFRREKYVPAGGPDGGDGGRGASI
                     IFEVDLGLRTLMDFKYQKKYQAQNGGDGSKGKRAGKNGENLVLKVPAGTVIRDEATGL
                     VLADLKKEGDTAIVAKGGIGGKGNQHFANAVRQAPAFAKSGTDGEERWITLELKMIAD
                     VGLLGFPNVGKSTFLSVVTKAKPKIANYHFTTLTPNLGVVQTKFGDSFVLADIPGIIE
                     GASEGIGLGHEFLRHVERTKVLIHIVDISGLEGRDPIEDFDKINDELKLYNEKLSKRP
                     QVVVANKFDILEDESKFEKFKSELEGRGYTVFKMSAATRQGIDEVIAYVSKMLKEVED
                     VELVSEEEMYRPELDIGTEEELSIDIEDGVYVVTGKALRRIMYSVNFDDMESLQYFQK
                     AMESQGVFDRLREMGIEDGDVVKIYELEFEFYN"
     gene            complement(11282..11572)
                     /gene="rpmA"
                     /locus_tag="ACOIAF_000641"
     CDS             complement(11282..11572)
                     /gene="rpmA"
                     /locus_tag="ACOIAF_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HGZ2667668.1"
                     /translation="MLNMNLQLLASKKGVGSSKNGRDSISKRLGVKRFDGQLVTAGSI
                     IVRQRGTKIHPGTNVGKGSDDTLFALVDGTVKFERKDKKRKKVSIYPVAIAE"
     gene            complement(11586..11903)
                     /locus_tag="ACOIAF_000642"
     CDS             complement(11586..11903)
                     /locus_tag="ACOIAF_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HGZ2667669.1"
                     /translation="MIKVKYYYNDDFLLQGFCLKGHADFAEIGYDIVCAAVTSNAIAV
                     INSLDKLQKIEFEKVVGEEGHIECIVKDTHVKDAQLLLNHFQLAVGEIKREYPKNIKI
                     LKK"
     gene            complement(11918..12229)
                     /gene="rplU"
                     /locus_tag="ACOIAF_000643"
     CDS             complement(11918..12229)
                     /gene="rplU"
                     /locus_tag="ACOIAF_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000270907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HGZ2667670.1"
                     /translation="MYAIVKTGGKQYKVSEGDVLFVEKLEANAGDVVTLNEVLACSKD
                     GELKLGSPVVEGASVQAKVVEQGKAKKVIVFKYKAKKDYRRKQGHRQSYTKIVVEKIN
                     A"
     gene            complement(12330..13751)
                     /locus_tag="ACOIAF_000644"
     CDS             complement(12330..13751)
                     /locus_tag="ACOIAF_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HGZ2667671.1"
                     /translation="MKKIVIESLIGSQKTAVLEDERLTELFVEDNLNKKTVSNIYRGI
                     VKKVIPGIEACFVDIGFKKLAYLQLKKGSTIKSGQDILVQINKEEIGTKGAKLNTEIS
                     ISGRYIVYIPSNDRTTISNKITDEKERFRLKKITKAVNKENLGLIIRTEAQGCTHDEI
                     KKDIEELKLKYENILKEYKLGIGPKLLYKSLDFATKYVKDNVNDDIESIITNSYDKYS
                     ELKSILRGIDKTYVDKLCLEENRDVFDLYRIESKIEKLLNKKVWLKSGGYLIIEKTEA
                     LTVIDVNTGKFIGTGKLDETVYKTNLEAAKEIVRQLRIRDIAGIIIIDFIDMHKKKHQ
                     KEILHILEEEFNKDKRKAEVLGMTKLGLVEVARRREKESIDKYYLMSCPCCDGEQTIR
                     SVHYILDSIEKEIMRISEHTVYKNIMVEFNDFIFEQIKEYYMDIIDKIGEKYNIKISL
                     NANSTLKHNKTNVIFDKIVDNKM"
     gene            complement(13769..14470)
                     /locus_tag="ACOIAF_000645"
     CDS             complement(13769..14470)
                     /locus_tag="ACOIAF_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HGZ2667672.1"
                     /translation="MSKIIRVKFKKEGDMIYISHLDLQRLLQRAFRRAEINLSHSQGF
                     NPHPKMSYGNALALGTESQGEYVDIEIEEDDLSVEEFLNKVSIQLPDGIDFIKAKEID
                     RQTPSLSSVIDYGEYLFNIDLKRPMTKEFVKRKVIDFMNNKEIIITKKNKKGKMVEVD
                     IRPMIRTFDVLNLEDEHITLTATIATGSKTNLNTNILIPKILEMFELDIDPLDVDILR
                     RDLYVLEDGELVTPM"
     gene            complement(14472..16316)
                     /locus_tag="ACOIAF_000646"
     CDS             complement(14472..16316)
                     /locus_tag="ACOIAF_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HGZ2667673.1"
                     /translation="MNKVDLKKILKKVEKPARYLGNEINSIHKDTSNSELIRYAHCFP
                     DLYEVGMSHLGSHILYDVINKDEDVFCERVYSPAVDMENIMREKSIPLFALESREPIT
                     NFDFVTFTLQYELSYTNILNILDLANIPILKEERTLEDPFIMVGGPCAYNSEPLADFV
                     DVVILGEGEEVNLEVVNEYKEWKKNKTTREDFLYKISSIEGVYIPSFYDVKYNEDGTV
                     QSVTPNREGITKNPTKRIIKDVETVDCPEKLIVPYIDTVHDRIVLELFRGCTRGCRFC
                     QAGMIYRPIREKSVKRLKEILDKLVKNTGYDEISLSSLSTSDYSKLSELTDYLVDEYA
                     SNNIGISLPSLRLDNFSMEIADKIQQVRKSGLTFAPEAGTQRLRDVINKGVTEEDLEN
                     ATERAFEMGWNSVKLYFMIGLPTETYEDLDGIAKLAYKVIDIYRKVNGGKLKRSFSVT
                     VSTSTFVPKPFTPFQWHGQDTTEEVINKQRHLVNKLRNNNIKYNYHDSKTSLMEAVVA
                     RGDRRIGKVIYDAFRLGAKFDGWAEHFNLDIWKEAMEKNNLSIDFYAHRNRNYEEVFP
                     WDHIDVGISKKFLIREDENAKKEKITSDCRHNCNGCGINIHDIGRGLC"
     gene            16635..17828
                     /locus_tag="ACOIAF_000647"
     CDS             16635..17828
                     /locus_tag="ACOIAF_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HGZ2667674.1"
                     /translation="MSKVFEVKKDIYFTGVVDEGLKVFDIIMETEFGTTYNSYLIKDE
                     KTVLFDTVKANFKDEFLSNLSEVTDIAKIDYVVIHHTEPDHAGSLKYLLDINPNIEVY
                     CTKAAKLYLDGQINRPFNCHVIKDGEILNIGKRNLRFITAPFLHWVDTMFTYIEEDKT
                     LLTCDAFGCHFASVDAEVVNSEDYLKSAKHYYDCIVKPFAKHVLSAVDKVVGLNIEFD
                     TILTSHGPMLTKDPMAAVKRYVEWSTEAVNTTNQNQVSIFYLSAYSNTLEMAKKIKEG
                     LDKEGAKAELYDLEDMTLTEMHDTLVVSKVILLGSPTINKTMVKPMWDLFSVIDPMAN
                     QGKIAGVFGSFGWSGEGITMAETLLKSMSFKMPVESLKKKFFPSEETLKECMAFGAEF
                     AKLVK"
     gene            complement(18040..19122)
                     /locus_tag="ACOIAF_000648"
     CDS             complement(18040..19122)
                     /locus_tag="ACOIAF_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HGZ2667675.1"
                     /translation="MKKIRKLATKLLIATMVLTSCMSVTVYGMTAKDVTAKTPKSYVA
                     GTNSIYGPKLSQAQLNSVAQATADFMNKKITKNMTTDAKIRVAYNHIKNNTTYVNWNA
                     AEGANTAYTLVTKKGACSGMCRSLKALCDAMGIECYYVHSTTNDHQWNLIRFEDGRLY
                     HVDIDANKSAGADIIYKSLTHPLPFDKKAYPAVGEVVKTQNPTPTVKKPTPAKPSAPV
                     GPGNHTMIADQQGAILDEVYGNNLRPKIEMYEGVPCSLINSSINTIKFTSKNSNLDPS
                     KHTVTVIGFMNTPYGMVALAPDGVPDEYPFEYDKPFSLDFTGKNLSAAELSKKCKENN
                     VIIKVEIGETASNSGRGMVAYARYVN"
     gene            complement(19686..20477)
                     /locus_tag="ACOIAF_000649"
     CDS             complement(19686..20477)
                     /locus_tag="ACOIAF_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HGZ2667676.1"
                     /translation="MKLIDEYLDKLYKKCDNKSTIELKQEMRCHLIESANEFKLEGLD
                     EEEACKKAIERFDDGDEMQYELCNIIKELSLSLDRHKSIVMGFKKVLGYISIIAFLIS
                     GFMWYYNNSLQHNMYNLGKELDGEIKQLAERHDMTKIGEYKLELEKILDKDKYSKVKA
                     LRLYVIDMKDGNTNLSSSGLNANMVYEREADYNNISNFIQHLGYNGKDFLDKNGNIVN
                     PDIFLEYFFYFESEMLIPVAFAFGLLCIIAYFILRFKISLIKNNN"
     gene            complement(20481..20810)
                     /locus_tag="ACOIAF_000650"
     CDS             complement(20481..20810)
                     /locus_tag="ACOIAF_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HGZ2667677.1"
                     /translation="MQLNKEVLKGYIDILIVSILEKKDCYGYEIAKQVRERSEFELKE
                     GTMYLALKRMESKNLIKSYYSNEQSSGGRRKYYNLTNEGKDFLEIKKQEWRFIKKVMN
                     QFLGEVD"
     gene            complement(21294..21707)
                     /locus_tag="ACOIAF_000651"
     CDS             complement(21294..21707)
                     /locus_tag="ACOIAF_000651"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HGZ2667678.1"
                     /translation="MNIALVAHDQMKNTMVGFCIGYESILKKYGLYATGTTGKRIMDE
                     TELNINRLASGPLGGDQQIGSLIVTQEIDLVIFLRDPLTSQAHETDIQALIRLCDVYH
                     VPIATNLASAEIFIKALDRGELSWREVRKSKSQRI"
     gene            complement(21748..22878)
                     /gene="rodA"
                     /locus_tag="ACOIAF_000652"
     CDS             complement(21748..22878)
                     /gene="rodA"
                     /locus_tag="ACOIAF_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HGZ2667679.1"
                     /translation="MKKININYKSTIKLIKQLDWKLIVTVLAIFIFGLVILSSATHAN
                     STGSYNQLIKQGLAFVLGIGMIIVILFFDYNLLGRYYKALYIISLILLAIVLLPGIGT
                     VKGGARSWINLGPLDLQTSEIVKLTFVLSYAKILESKKDKLNTLKEVMPVVVYSLPFI
                     GLLIAQPDLGTGIVFCCMIFAMLFTAGLSSKLIKRGIIILLVSMPLMYLMMADHQKVR
                     IEAFLNPEDVTLKGNYQVMQSLIAIGSGGVTGKGLYNGSQNQEDFLPVQDSDFIFAVV
                     GEELGVIGMAVLIILFMIFLLRLLAIARDAKDFYGTLIVVGVMGMFGYQIIQNIGMTV
                     ALIPVTGVTLPFVSYGGSSLLTSLANLGLVLNVCMRRKKINF"
     gene            complement(22963..23247)
                     /gene="minE"
                     /locus_tag="ACOIAF_000653"
     CDS             complement(22963..23247)
                     /gene="minE"
                     /locus_tag="ACOIAF_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HGZ2667680.1"
                     /translation="MLDLFRVFSNEAKTSKSVAKERLKLVLVHDRVDCSPQLLEMIKT
                     DILKVIANYAEIEDDGLEIKMSKCRGEHDDKPVSALVANIPLKNIKDRCM"
     gene            complement(23265..24062)
                     /gene="minD"
                     /locus_tag="ACOIAF_000654"
     CDS             complement(23265..24062)
                     /gene="minD"
                     /locus_tag="ACOIAF_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HGZ2667681.1"
                     /translation="MSEVIVITSGKGGVGKTTTAANLGTALSLENKKTVVVDADIGLR
                     NLDVVMGLENRIVYDIVDVVEGTCRLKQALIKDKRFDNLYLLPAAQTRDKNAVSVEQM
                     IDLCEKLKESFEYIIIDCPAGIEQGFKNAVAGADRAIVVTNPEISAVRDADRIIGLLE
                     ANEIKEIRLVINRIRNDMVKRGDMMDKQDIIEILAIDLLGLVPDDESIIISTNKGEPA
                     ILDSKSLAGQAYKNIAKRILNEEVPLLDLEVEDGFFGRLKKMFSMAK"
     gene            complement(24086..24769)
                     /gene="minC"
                     /locus_tag="ACOIAF_000655"
     CDS             complement(24086..24769)
                     /gene="minC"
                     /locus_tag="ACOIAF_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HGZ2667682.1"
                     /translation="MSLREICSQELVEFKGNKRGIIVNIKREAPFEEIQEKIINKLEA
                     YVGFFNGAKISKINSDCLTDMEILELKEGITSRFDVEFVEDQKIEENSNFPTKYVNTL
                     RSGENIEFEGDVVILNDMKPGSKVLSKSNTVVMGDINAGAKVVAGGNVFVMGKIEGFV
                     HAGAEGNEFAYVVAGNLNPKILQIADNIAEAPDDEENYESESEISPEIAFVSNGRIVI
                     ESYLSKLDK"
     gene            complement(24867..27845)
                     /locus_tag="ACOIAF_000656"
     CDS             complement(24867..27845)
                     /locus_tag="ACOIAF_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HGZ2667683.1"
                     /translation="MEENKQVDRLKIIKIVMGVIFLAILVKIIYMTTFKYEYYNELAE
                     NKTYKKLAIEAPRGEIKDRYGRLLAGNKNLFTVQVSGNDINKKDANKHSRANEISLKL
                     INLLERNGEEYVDEFPIYVENGKYYYTYDRDIREYKSENGIPNDYNAKESFYYLVDKL
                     ISAGILSQEDKRLDATRLQAKLNENGYYPPILVSKWMFTAERDKRDWLASYKIKETKL
                     SAKEAFEKVRNSDALEIDKSLSDEDARKIMVVRDLIKSKGYSQYNPVTIAKDVGETTI
                     AQIEESAMDLVGVSIAVEPVRYYPNGSLASHMLGYVGKMPSTQIESYLQKGYETGDMV
                     GLAGVEKSNESRLRGTDGYKMVKVDALGRISKEIESKKPKSGDTVYLTLDKDLQEVSD
                     NALKQIIEVASKGGTFKSKFGDKPISAYAGKAQSAALIAIDVKNGEVLASSSYPNYDP
                     NKFAKGISTEDYKALQPKNPNDLLAGSPLLNLVTQGEFQPGSSFKMVTSMAALENGLD
                     PNFTINDPGVIMLGKKSFGDYVWNHGRGNHGMTNLYKAIQESCNIYMATIGTGKTWPD
                     GKSIGIDMNANKILEYAKLFGLDQNTGLQDEVEERAGKVPSTEDKLKSTQALLKSNLE
                     REMANDFVDITREKNPKEYEKRINEIVSWAAEKKTPGRVETMNRLKKMNVKEDRIEEV
                     ADLAVFSYFNFAKWSTADTFNLAIGQGENAYTPAQISRYVAAIANGGNLVELSVVDRA
                     VSSDYSSVKINDQKKVEKIPFKNPDNLKELTKGMKLVARQGTAKSAFADFPIDVAAKT
                     GTAEKSGKIPTDNEYEYLKSHMSSYNVNLNDAIKLADKMKAEKEKELSLAKEKEIKKK
                     LENKDLKDEERKKLEEELEDGVKVRLEDTDKVNSSYLRKAIKELNPKITDDQIDRFKQ
                     DYGSFTWTVAFAPADDPEIAVVCVIPQGDSSVFSLLPTREVIGTYMGLEPTNSKNDNK
                     TNDVNNSSDENINFESQINR"
     gene            complement(27857..28345)
                     /gene="mreD"
                     /locus_tag="ACOIAF_000657"
     CDS             complement(27857..28345)
                     /gene="mreD"
                     /locus_tag="ACOIAF_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HGZ2667684.1"
                     /translation="MKKVLLCLLGIVLMTIENSITNYIDIFGISFNLVLIYVTIISLY
                     LDELEGGIIAAIIGLIKDITVGGIFGVNALILFIIAYAIGYMRDQLYKGSYIITFVLI
                     FIGSLIDSIVNIGTSSIIYQSYNISTLFVKGLLVAPIVNSLIGLVIYRLSKRAVLKLK
                     ED"
     gene            complement(28360..29253)
                     /gene="mreC"
                     /locus_tag="ACOIAF_000658"
     CDS             complement(28360..29253)
                     /gene="mreC"
                     /locus_tag="ACOIAF_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HGZ2667685.1"
                     /translation="MALRFDKNKNEKKRINVKVIATGVVAITLIGIVGISIGKFSSGS
                     PVNLGVASDAITSVGKGINDGFSFIKNGFKDVANFKDNSKKVKKLEEENEKLKKNMIA
                     LNAKLDKTESLEELKKTLNFVQEEYKATSISASVVGKNDGNWYESFVIGAGKNSGVKK
                     ESIVMNGSGLVGIVYEVSNNYSKAISLLDSKASVSFKLAKDANAKGTITQNTTLDNKD
                     SYNSKGYLQGYMFDSSYNVIQGDIITTSGLGFFPDGIPIGEVEKVVDDKDKSLKYVVV
                     KPYVDFKNINDVVVIEPRNIG"
     gene            complement(29263..30324)
                     /locus_tag="ACOIAF_000659"
     CDS             complement(29263..30324)
                     /locus_tag="ACOIAF_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HGZ2667686.1"
                     /translation="MAKEKKKEKKGFFSFNKMTKDMGIDLGTANTLVYIKGQGIVVRE
                     PSVVAIRDDSKEVLAVGEEAKKMIGRTPGNIVAIRPMKDGVIADFDITQSMISYFIQK
                     AADKKGVVSPRIAICVPFGVTEVEKRAIEEAARQAGAKDAFLIEEPMAAAIGAGLKVE
                     EPEGNMVVDIGGGTSEIAVISLGGIVTAKSIRIGGDEFDESIVAYVKKEYNLMIGERT
                     AENVKINIGSTFKDDEEINMQIRGRDLISGLPKTIEICSTEVREALKEPVSSIVDAIK
                     STLERTPPELASDIMENGIMLTGGGALLRGLDKLITQETGMSVQIAETPLDCVALGTG
                     KSVEDQEIFEKVLMMNTKN"
     gene            complement(30394..31053)
                     /gene="radC"
                     /locus_tag="ACOIAF_000660"
     CDS             complement(30394..31053)
                     /gene="radC"
                     /locus_tag="ACOIAF_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HGZ2667687.1"
                     /translation="MAPEERPREKMLAKGVKSLSNAELLAILLRTGNKNKNAIELANY
                     IINRDIQGIRHLEDMTIEELCNIDGIGLSKSTQIKAALELGSRVASFKPIKYKIRNPW
                     DIQRYYMDSLRYLKKEVFKAVLLNTKNEIISDVDVSIGTLSSSLVHPREVFKEAIRRS
                     ASKIIVMHNHPSGSVEPSREDKNITSRLIKCGEIIGIEIIDHIIIGDGLYFSFKENMI
                     I"
     gene            complement(31098..31682)
                     /locus_tag="ACOIAF_000661"
     CDS             complement(31098..31682)
                     /locus_tag="ACOIAF_000661"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HGZ2667688.1"
                     /translation="MNIILASASPRRKEILENTNVRFDIIKNEIDEIILEGETPKHLV
                     MRLAFEKSMSVASEHNEDVVIGADTVVVLDNAILGKPKDESCARDMLKRLSGREHQVI
                     TGISLINLCEDKKVIDYVISNVKFKTLSEQDIEDYLKTNESFDKAGAYGIQGYGALLV
                     EEIRGDYFNIVGLPISRLGDLLKKYFSINLFYGV"
     gene            complement(31902..32873)
                     /locus_tag="ACOIAF_000662"
     CDS             complement(31902..32873)
                     /locus_tag="ACOIAF_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HGZ2667689.1"
                     /translation="MVILTAVLVLGIMGLIFGIVLDFASKKFAVEVDERVEAILGVLP
                     GANCGGCGFPGCGGLANAIVEGNAPVNGCPVGGADVGAKVGEIMGISAEAGEKQVAKV
                     ICKGTCSSAKDKYEYEGISDCRAANVLNSGAKMCKFGCLGLGTCKDACKFDAISIVDG
                     IAVIDEEKCVNCGKCKEVCPKGIIITKPESQEVVVECNSKEFGKAVKEKCTAGCIGCG
                     MCVKACKFDAIIFEDKIAKIDPNKCVGCMQCVAKCPTKVISGDITKKKKVTIDQELCV
                     GCTVCKKQCKFDAIEGELKEKHKVDADKCVGCHLCMEKCPKKAIKIL"
     gene            complement(32889..33464)
                     /gene="rsxA"
                     /locus_tag="ACOIAF_000663"
     CDS             complement(32889..33464)
                     /gene="rsxA"
                     /locus_tag="ACOIAF_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HGZ2667690.1"
                     /translation="MNLILLFLSIVLVNNVITSQFLGICPFLGVSKKVDTAVGMGVAV
                     TFVLTLASIITYFIQILLVKTGTGFLQTIAFILVIASIVQFVEMVIQKMSPSLYQALG
                     VYLPLITTNCAVLGIALVNVQKSYNLVETIINGFGAGAGFTLAIVIFAGIRERLELAD
                     IPEAFKGFPITLISAGLMSIAFLGFTGLIKL"
     gene            complement(33479..34072)
                     /locus_tag="ACOIAF_000664"
     CDS             complement(33479..34072)
                     /locus_tag="ACOIAF_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit E"
                     /protein_id="HGZ2667691.1"
                     /translation="MNLAKVFKNGLIDENPTFVQVIGMCPTLAVTTSAINGIGMGLST
                     AAVLICANLVISLIRKITPDKIRIPIFIVVIATFVTIVGMLLKAYVPALDKALGIYIP
                     LIVVNCLILARAESFAFKTGAMPSIVDGVGQGLGFTVALTIIGAVRELLGNGSLFGMT
                     LFGASFQPVLIFILPPGAFLTLGFLFAGFNKLRSKKA"
     gene            complement(34085..34654)
                     /locus_tag="ACOIAF_000665"
     CDS             complement(34085..34654)
                     /locus_tag="ACOIAF_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HGZ2667692.1"
                     /translation="MNSMVRLGGTLLAISAIAALALGATNQVTAPVIEQRNIQANNEL
                     RKAVLPEAKEFKEMKEDTYKGLGDDLIAEVYEGLDGSEVVGYTLKAKPSGYGGEIEVM
                     VGISSEGQVTGVDIGNMSETAGLGAKAKDDAFKGQYKGKTAEPLEVAKGSTTADNQIL
                     AISGATITSTAVTTGVNAAIDVFNSALNK"
     gene            complement(34654..35631)
                     /locus_tag="ACOIAF_000666"
     CDS             complement(34654..35631)
                     /locus_tag="ACOIAF_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HGZ2667693.1"
                     /translation="MENKLIVSSSPHVRSNEDTSYIMKQVIIALLPAAVAGVYFFRLN
                     ALSAMFFCILGTVGTEFLYQKFTKQKSTIGDFSAVVTGLLLAFNVPASLPWWMCLVGG
                     IFAILVVKMVFGGIGCNFVNPALAARAFLLASFPVAMTAWTQPGVNWIGKNLDAVTTA
                     TPLSFLKNGAAGLADLSSNGISLADMMIGNIGGCIGETSAILLLLGGVYLMYKGIINY
                     VIPVFYIATVFILTFLLGGFNITFAIYQLFAGGLMLGGFFMLTDYTTSPMTKKGQIIY
                     AVLAGLITTVIRMYGGYPEGVSYSILLVNCLAPLIDKFVRNRVFGEVAK"
     gene            complement(35654..36991)
                     /gene="rsxC"
                     /locus_tag="ACOIAF_000667"
     CDS             complement(35654..36991)
                     /gene="rsxC"
                     /locus_tag="ACOIAF_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HGZ2667694.1"
                     /translation="MKLLTFKGGIHPPYRKEYSNTKALEKAQAPKIVYIPLQQHIGAP
                     AKPIVEVGDEVKFGQKIGEQQGFVSCNVHSSVSGKVIAIEQHEVPGGSAQCVVIENDF
                     KEELHESVQPKGQLEDLSKEDIVGIIKEAGIVGMGGATFPNHVKVSPPPDSKAEVVIL
                     NGAECEPYLTADHRLMVENPEDVVFGLRALMKVLDVKKGFIGIETNKPDAIEAIQNVA
                     KDYSEIEVVGLQVKYPQGAEKQLIYACTGKEVPSGGLPIAAGAVVDNVATAAQIAKSI
                     KTGMPLVERITTITGSCIKEPKNLITKVGTLVSEIIEQCGGFKEDKKIGKVIMGGPMM
                     GIAQYTTEIATNKGSSGILCLDEEESRTPDIQNCLRCGRCTDVCPSFLQPLFISAYSL
                     KDDYDTAEYHRAMDCIECGSCSFICPARRPLLQSIRSAKREIGAKRRKQAAQK"
     gene            complement(37207..38151)
                     /locus_tag="ACOIAF_000668"
     CDS             complement(37207..38151)
                     /locus_tag="ACOIAF_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667695.1"
                     /translation="MNRRKTIYRGFNNNRHKIDLRKYVITIACLCLIGYYSYTKIKDS
                     KILEYVSAKIPFLNNSSDITYKDISDELNSIKKGKKSKSQTNSDDKQETNPEKAVNNT
                     KEPEEVKLATIEGWDMYTIQVAAIDNNDDLKKIQTSLVNNDIPFSVMEKDGVKKIQTY
                     SSFDENDVRKQISSVRKVFPDAFLSHLDAPMLSLEYTSNYAYIESISKELNKLITNFK
                     EESSFWSNAENNVDMEKYNTILTNRKAISQNISKEAEKIDYSEMRLFKDNLIEYVKNV
                     NEKIDTASKAANEEKYSVSKSLLLSSMQEYSMFINSIK"
     gene            complement(38262..39536)
                     /locus_tag="ACOIAF_000669"
     CDS             complement(38262..39536)
                     /locus_tag="ACOIAF_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HGZ2667696.1"
                     /translation="MKKAIAALGIGAVAVSVSSINASALEKGTVTASALNIRSGPSSD
                     CDKVAKLYKGKTVEILEKSNGWYKVRVSSSVVGWGSAKYISTSGSSEGTSSQNNSTSS
                     GTTISGNGKVNVSSRLNVRSGAGTNYSLVGKANNGDVVKLLEQSNGWYKIKLSNGVTG
                     WASSQYISKTSEDVGTNNSSNSNSTNNSDKKPSSEESIEGKNGKVTSAVSLNVRSGPG
                     TSYSIIGKLNGGDVVELKAKSNGWYKVKLSSGTIGWVSASYISETNEDTKEKPNSSSN
                     QNSQSNSNSKPSFTGNSDKSTAKGSTIVDFAYTLIGIPYQWGASGPDKFDCSGFTQYV
                     FKHSVGVSIPRVSREQANFGSAISMGNYAPGDLVYFDTDGDGTTNHVGIYVGNSKFIH
                     CSGTQTNPNKVKVDNLTSSYWSKVLLGARRFV"
     gene            complement(39668..40039)
                     /locus_tag="ACOIAF_000670"
     CDS             complement(39668..40039)
                     /locus_tag="ACOIAF_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGZ2667697.1"
                     /translation="MKFNFCHNNFNVTNLEKSLDFYKKALGLKEVKRKEAEDGSFILV
                     YLGDGITSHTLELTWLRDWDRPYNLGDNEFHLALEVEEFDEAKKLHKDLDCICFENES
                     MGIYFIADPDNYWIEILPKNH"
     gene            complement(40267..40866)
                     /locus_tag="ACOIAF_000671"
     CDS             complement(40267..40866)
                     /locus_tag="ACOIAF_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667698.1"
                     /translation="MESRGYKEDLKKMILDMAHKELESYVSQKGAKKYFEGNLDKIIN
                     NNIKKFNQSIGLNRVYLKMLKKCAYQESVTSISRTIKKEALFKSKKELFAFSEYLKLD
                     INKRLSYNQILRKISRYIYYNRSSYAQKYVVFKRGDEEYLLEPEKIKDELISSYRSKT
                     REDMRSIAKLLDIEVEDDYNAEDIRKKVINYIIKEKLKS"
     gene            complement(41067..41279)
                     /locus_tag="ACOIAF_000672"
     CDS             complement(41067..41279)
                     /locus_tag="ACOIAF_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HGZ2667699.1"
                     /translation="MKKKLLVEGMSCGHCVNHLKTALTEDIDGIEVLDVDLENKCASV
                     DMKDDVSIEKLKEVIAELGFELKGIE"
     gene            complement(41352..43067)
                     /locus_tag="ACOIAF_000673"
     CDS             complement(41352..43067)
                     /locus_tag="ACOIAF_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGZ2667700.1"
                     /translation="MKRIKVLTVVLAITLMVAGCSNTKTKQSSSDSSLQSQSSIDAKY
                     INLTMVTPKTINPITNTDKSVGYIMNLVYDSLFTIDENYNVIPQLVKEYNIAQDGMSI
                     DIKLKDAKWHDGKNVTSNDVNYTIGLIQKSADSPYNEFTKNIASVNIKSDKDFTIKFK
                     ARYAFSIDSLIFPIVSQNHLDSKDVNDKNNNMIGNGKYKIESYTEREGMVLSVNKDYY
                     EEVPKTMKNIKVGMVPNEDARTSMVMALDSDITNVTLNDLSKFQEKEFNITKYQGRDY
                     ECVLFNYNNPFFKDVNFRKAIAHSIDKDRIISEGYMDDATPVNFPLNSKSKYYNSEMK
                     DLEFNKDKAIECLAKVEYANVNSVNQNDNKVKNQKKSAKKNSKKLTPEEEAKAKKAEE
                     DRIKKEAEEKKNREKEEVKKSLSEMNLKIIVNRDNSERIKTANIINENLKAIGINSTV
                     NQLSDKDMENALNSKNYDLALVGWKLSIIPDASSIIASSGYTDDKLNGYMSALTSSTS
                     EIETKKAYKDVQTYIKDNATFISLAVRNNYIVSNSRLKGKITPNDFDVYEGISNLDIK
                     SNESN"
     gene            complement(43067..43621)
                     /locus_tag="ACOIAF_000674"
     CDS             complement(43067..43621)
                     /locus_tag="ACOIAF_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HGZ2667701.1"
                     /translation="MNSKKVLILSIFIILFGALLMESKVIHKFLYPKKYSEYVEKYSK
                     EFNLDENIVYSVIKAESKFNSSAVSKKEAKGLMQILDITRDWGAEELNLKNVDIFDPE
                     TNIRLGCWYLSKLYKEFGKLDLVIAAYNGGSGNVKKWLENNEYSKDGENLHDIPFKQT
                     SKYVEKVKNNYEHYNKIYGKKGKN"
     gene            complement(43614..44216)
                     /gene="coaE"
                     /locus_tag="ACOIAF_000675"
     CDS             complement(43614..44216)
                     /gene="coaE"
                     /locus_tag="ACOIAF_000675"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419019.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HGZ2667702.1"
                     /translation="MLILGLTGGIGCGKSSLSNIFRNLNIPIVDADIISRKIFEDKLL
                     LEKVFVHFGQSIKNDDGTLNRKALGKIVFSDEEKLKELNNLTHPRIREKIISEIEKLR
                     KKGENIVVLDAAILVESGFLDMVDKLLVVTCKQEVQISRIQKRDNCSEQEALSRINSQ
                     MSQEEKSKYGDYIIDNSGTITELESKAHKFIEYMKENWRE"
     gene            complement(44225..46873)
                     /gene="polA"
                     /locus_tag="ACOIAF_000676"
     CDS             complement(44225..46873)
                     /gene="polA"
                     /locus_tag="ACOIAF_000676"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HGZ2667703.1"
                     /translation="MEKKLIIIDGNSIINRAFYALPEMNNKEGLKTNAIYGFTTMLFK
                     MIDIYKPTHISVAFDRKAPTFRHLEYKEYKAGRKGMPDELAEQLQPLKDLLDKFKINR
                     LEIDGYEADDIIGTVSKKAENNGYKVYIVTGDKDAIQLASDNTTTLITKKGVGEVEEY
                     NFNSVIEKYEMTPTQFIDLKGLMGDKSDNIPGVPGIGEKTGIKLIKEFSSIENLIENT
                     DKLKGSVKKKIEENKEIAIQSKRLATIIRDVPIEVDLDSMIFGDYDKDELLDKFRYFG
                     FTSLLSRLVDLVDSDDTEHVEEKIEILKLKDIEKFIDEVNKKGQVILKTVRGQGNILE
                     KNIIYIFISVDGEKIYYVDSNEVSKLDSIFSNNEIKKYGYNLKDDYISLKPYNINLEN
                     MYFDITIAEYLIDSTSSTSYDCSAIAMKYLSKKVKSKEELLGKGVKSKNYEDLEFEDL
                     AEYMGQIICTVKETVLMMERSLMDMSMDGLFYHVEMPLVEVLGHMEYEGIKVDKSILD
                     ELSVEFKEIIATLEKEIYELSGEPFNINSPKQLGVILFEKLELPIIKKTKTGYSTNAD
                     VLEKLRDKHPIIDKITEYRQIVKLNSTYVEGLLNIINPESNRIHSSFNQTITTTGRIS
                     STEPNLQNIPIKMEMGRKIRKVFIADDNCKLVDADYSQVELRVLAHMSQDENMIEAFK
                     HHEDIHTKTASQVFNVPMEDVTSELRGAAKAVNFGIIYGKSDFGLADDLNIPVPKAKE
                     YIESYFAKYHKIKEFMDTTVEKASEDGYAVTILNRRRYIPEIKSSNFMERNRGKRFAM
                     NAPIQGSAADIIKIAMINVHKKLEENNLKSKLILQVHDELIVEAIDDEIDIVKKIVKE
                     EMENAVNMDVHLDVDLNVGSSWYETK"
     gene            47025..47112
                     /locus_tag="ACOIAF_000677"
     tRNA            47025..47112
                     /locus_tag="ACOIAF_000677"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:47059..47061,aa:Leu,seq:caa)
     gene            complement(47299..47742)
                     /locus_tag="ACOIAF_000678"
     CDS             complement(47299..47742)
                     /locus_tag="ACOIAF_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667704.1"
                     /translation="MKKNMLYVGIIYFICGILLVLLATFTEFSFEAFIWGLSGAALGP
                     GVCMILQYMYWSKPERAVDYEEKIKNQRIEMNDERRIMLRDKAGRITNQIMCYVLVIL
                     IFIVSILSVFSVMVISKWVLVVLGLLILFQFICSVVVYNKLDKRL"
     gene            complement(47745..47942)
                     /locus_tag="ACOIAF_000679"
     CDS             complement(47745..47942)
                     /locus_tag="ACOIAF_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGZ2667705.1"
                     /translation="MKNRLEEIRKERGIKQEELASILQVSRQTIGSLENGRYNPSIIL
                     AFKIARYFDMSIEEIFIYEEE"
     gene            complement(48134..49000)
                     /locus_tag="ACOIAF_000680"
     CDS             complement(48134..49000)
                     /locus_tag="ACOIAF_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HGZ2667706.1"
                     /translation="MEWINKLNQAITYIEENLESGVDYAEAAKIACCSTFHFQRMFSY
                     IAEVPLSEYIRRRRMTKAAFELQNSNIKILELSEKYGYDSPTSFNRAFQNIHNISPSA
                     ARAKGVVLKAYPKMTLSIYVKGNIEMQYRIVEKKGFRIVGIKESMNMIVEECFEKVPK
                     LWAKCIKNGTIDKLSELINNEPCGLLGVSVCTNSKGLDYYIAAPTDKPVLEDTHEYLI
                     PSGTWVVFECVSPMPNALAIQELQKRIITEWLPSSGYVYANLPDIELYPNGDINAPDY
                     TTEVWIPIQKPS"
     gene            complement(49226..50095)
                     /locus_tag="ACOIAF_000681"
     CDS             complement(49226..50095)
                     /locus_tag="ACOIAF_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HGZ2667707.1"
                     /translation="MDYKTIILNRKSVREYKNTDIKEEYLSEIKKYSESCKKLVPEID
                     VDVRIMNRSEVFNNLDKVAGYKGNMIDSPSYIIILSDIKDNYIENSGYIGESIIFKVT
                     ELGIGSCWVTFEDGKKVKEKLGINSDKEVTGIIAIGYGENVSNAKVLNATKTGQNYSK
                     SDMQVVTDKGSTRLGVEDVVYMNEWGNVASIEILEERALLDAFHYARLAPSTLNRQPW
                     RFIVDGGTVVLAVRKDGHTNLYEEKIDIGIVMLYFATIISATMFDLKWNLGTPDKDYK
                     VPEDYKIVGYCNI"
     gene            complement(50601..50822)
                     /locus_tag="ACOIAF_000682"
     CDS             complement(50601..50822)
                     /locus_tag="ACOIAF_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667708.1"
                     /translation="MNVNNHKCPECGEHSKKFEIVEENEDCVIFECYTCGTISILKYC
                     ENCKEFVFVNIPNNVETYEYCCGKCNNKI"
BASE COUNT        15786 a         9573 c         5579 g        20121 t
ORIGIN      
        1 tttcctggat ttaaaatatt tttagggtca aaagctaact ttatattttt aattaacatc
       61 atataagcat ctgaatttga ttcatgtaag tactcttttt tagcatatcc tatgccatgt
      121 tcaccagaaa cttgaccact aagttctcta gattttttat acatatagtc aaaagtttct
      181 tttaatctta ttttccatgt attatcatcc ataccatctt ttaaaatata tatgtgtaga
      241 tttccatctc ctgcatgacc aaaacttttt attcttattt ttaatttgtc ttgaagctcg
      301 tgagtgtatc ttataaactc tgctatctta tctcttggaa caacgacatc acattcatcc
      361 atttgtgtcg tagatgcctt tatagcttct aaaaatgcac ctctggctga ccatatagaa
      421 tcgtttcttt cttgtgtatc tgatataaat acatctaatg caccattttc taaacataaa
      481 ttagctactt tttcatattc tttttctata tcttcagtgc tatttccatc aaatgtaagc
      541 aaaagataag catctgatgt attatctgga aactttttac ctaaaaattc ttctgctgcc
      601 aatattacat ctctttccat aaattctata gccgttggta ttgattttga ttttattatt
      661 ttaggaacag tttctattgc cattgataaa tctggaaatg gtataagcaa acttatactt
      721 ttctttggta aaggaagcaa ttttaatata gctttagtaa ctattcctaa agtaccttct
      781 gagcccacta ataaatcctt tatactatat ccagaactat ttttaactac ctttccacct
      841 acatttataa tttctccatt tggtagtaca acttcaagac ctcttacata atcccttgta
      901 acgccatatt ttactgctct cataccacct gcattagtgt ttatattacc tgcaattgta
      961 gcactttttt cacctgggtc aggtgggtaa aataaatctc tatcttgaac atattgacct
     1021 attgtcatca ataaaacacc tggttctact gtcaatgtta aattttcata atcaacttca
     1081 agtattttat tcattttaca tagattaatc attattccac catttatggc aacagcagca
     1141 cctacaagac cagtaccttg acctcttggt gtgactggta tattattttt atatgcgtat
     1201 ttcattattt tagatacttg ttcagtttca agtacattta ctaaaacttc agggtacttc
     1261 tcaatccctc caagttcatc atgtgaaaaa tcttcattta tatcacttcc aactaataca
     1321 ttttcttctc cacaagtatc tattaaaaaa tctatatctt ttttatcaat taatttatac
     1381 atttctatcc ccctatttag ctgctgatgc catatatttt tgattattat ctttgttatt
     1441 ttctattttt tcaatcaatt taggaataat atcataaata tcacctatta aagctaaatg
     1501 tgctacatca agtataggcg ctttatcatc tttatttatt gctactatat agtcagcacc
     1561 ctgcatccct gcaacaaatt gaacagctcc agatacacca caagttatta tcaatttagg
     1621 ttttacagtt ctaccactaa gacctatttg ttttttagca tctatccaac ctgcttctat
     1681 tacaggtctt gttcctgcca cttgagcgtt taacttatca gctaatttat atatcatctc
     1741 catatcttct tgttttttta ttgctctact tgccacaact atcacttcag cttcttccag
     1801 accaacttct ttattttttt cttttacact cagtacctta attttagatt ttagttcttt
     1861 tttttctact ttacataaag ttatctttcc tgtagtattc tctatttttt ctggtgctga
     1921 aaatatttta tatcttacag tggcaaattg tggtctatta ttaggtgtgt ttatatgagc
     1981 cattatattt ccaccaaatg ctggtcttat ttggtctaaa tcagtatttg attgaatatc
     2041 aagtattgta caatctgctg taagacctgt tctaaatcta gcggcaagtc ttggagctaa
     2101 tgacctacct aatgtagttc caccaactaa tactatcgtt ggttttattt tatctataaa
     2161 gttttctatt gcttttgaat atggttctat cctaaaatct ttaaattcat cttcatcata
     2221 tacaaatact tcatccaccc cataagaaat caaattactg cacttatctt ttatgtcttt
     2281 tcctacaaat acacaataca caggttgttt tatttttgat gccatctcac gagctttacc
     2341 tataagctcg tacgttacag gatgtatatt tccattatga tgttcaacat atactgcaat
     2401 tcccctccat tcatctttat taatttgtac tttactactc tctataaatt caaatgcgcc
     2461 ttttggtcca cttttgacac acaacttgca catcttacag ccagcactta tttctactat
     2521 acctgacttt tcttctatag ccccaaaagg acatatttct aaaattctct ttaagtcagt
     2581 tattttatct tgatttacaa ctatctttga catttattac tcccccttac accaacttta
     2641 attcctctaa tttatttaca agtgcaaaac ttagttcatc tgcatttcct cttacaatat
     2701 ttttatctgt atttggcttt ggaggaaata ttcttacaac ttgagtagga gaaccattaa
     2761 gtccatagtt attttcattt ttatcttcaa agtcatctaa gctatataca ggtatttctc
     2821 tatctttagt attaagtttt aatttaaatg aaggtagtct tggttgaaaa atgtcttttt
     2881 ctacagttat taaacatgga tattcaatct cacatacttg taaatcattt ggcatatcta
     2941 tttcaacaac tatactttca tctttaactt caattaattt tgtaacgtta gtaacatgag
     3001 gtatatctaa aaattcagct acttcaggtc ccacctgtgc tgtatcacca tcagtagttt
     3061 gttttccaca gattattagg tctacctttc ccatcttctt aataccttgt gatatggtat
     3121 aggaagtagc caatacatct gctcctccaa atctcctatc acttattaat gctccttcat
     3181 cagctcccat cataaaactt tcttcaatta ccttctttgc ctgaggagga cccatgctaa
     3241 gtacttttag attcgctttc ttatcttcct ttattctaag agctgtttct aaagcatata
     3301 ggtcataggg attcattttt gaatctactc catctctttt tagtactcct gtagtttcat
     3361 caacttctac tgaagtcgtt ccaggaactt gtttaataca tgtcaaaatt tccataaagt
     3421 acactccttt tttatttaac tataactgat actccatcag gtataactac tactttgctc
     3481 tccttaccaa ctatactatt cgccatttct aaagcttctt taaagttaaa tgcatgtttc
     3541 atatgcattt tttttatcat ttcaggatta cacttatctg ttaccattat cacagtatat
     3601 ttagataata ttctcgctag tatctgaaat tcccattggt ctggaatcgt atctactcta
     3661 tcgacacttc taattttatc taaaagttgt tttggagaat ctgcatttgc tacattttca
     3721 taaaaagatt gacctccatg accatcatta catgctgaaa tcattattat tactccaccg
     3781 tctttacaag tagcttcagc tgctgtcatt cctttaacag attgataaat attttggtct
     3841 agtggatatc caccatttgt agttacaaca atatcagatt ttatagaatt tacttttgat
     3901 aactctgtta caaatctaca tcctttagta tgtgcttttt cgacatcacc agcaaaggaa
     3961 gcaattattt ttttatcttt atctattaca acattcaata tgaatgctaa ttttgcaact
     4021 ttagctgcat ataccatatc ctcatgaata ggattattat tcaattttcc agttcttgaa
     4081 ttatcactat ctataaagtc actacagtga ttgtacatta tagtctttgc agatgcgatt
     4141 cctggaagta cgctttttct gccacctgaa aaacctgcaa agaaatgagg ttctataaaa
     4201 ccttctgcta tcaaaagttc tgcctcatat gctagtttgt ttatatataa atctccacct
     4261 gaaggcaata tccctacttt acacatagat ttatcatcag tagatatatg cataactata
     4321 tcttcattat ctactatttc ttctcccatt ttataaatta gctcttccct tgtacttggc
     4381 ctatgaaatc cagttgccac tataatcttc acatcaattt ctggattaac attcctaatc
     4441 cttcttaata taattggcat agttattcta cttggtacag gtcttgtatg gtcacttgta
     4501 attatgacta cattcttttt ccccttaacc agttcttcca gagaattaga gcctatagga
     4561 ttattcatag attcttcaac tatctcttct tgagtcttat gttctatata attatccgat
     4621 tttgactcca aaacaccgag aaaacgttca tcaggtattt ctaaaatcat tccttgtttt
     4681 gaatacggta cttttaactt taccattttt aatctcccat tataataaac tataaaattg
     4741 gtatgtggtc ataccagttt tacatttata tgatagtacc attatctacc atttgcaaga
     4801 ataatttatt ttataacaaa cttttaattt tcattttttc tataattttt acttataaaa
     4861 tccatatgtt cctttgcttt ctcacatgca agttctacat cattttctac aactgcattg
     4921 tatatagctt catgctgttt aagcaatata ttttcattaa aaggttctgc gattattttt
     4981 tctcttgcat cttctataaa tccatcaaat aattttgatg ctgtcataaa aagactttga
     5041 attaaataat ttttacttaa tgttgctagt ttactgtgaa atttttggtc taataacacc
     5101 agtactcgat tattatcttt tttattagtt tctaccctca tttcatctat tatatgtttc
     5161 aattctataa cttcagaaga agttattctc tttgaagcaa gttttgcaat ctcaatttct
     5221 aggattattc taagctctaa tatatcttca agtgtaccat tattaagttt aaaaatcata
     5281 gataaaggtt ctattaaagt tttttctata tttgtacata taaaattacc ttcaccctgt
     5341 ctgctttcta tcactcccat agtctctaat accctaatag cttcccttat ggaagttcta
     5401 ctcacattca tttgttcaga cagttctctt tctgatagta atttatcccc cttttttaac
     5461 tctccattta gtattttata ttgtatttgc tcaattactt gttcataaac ttttttacta
     5521 gctatactat taaacatttt ttcctccaat atgataatcg ttgttgtaat atatatctat
     5581 tatatatctt aagccattac attcatatat ttttattact tttaatataa tatcttataa
     5641 ctcaaattta caggtgatat tatgaaaaaa aataaaatta ttgctttttt tgctccatct
     5701 atcatagttg gagttctagt tttagggatt atcctatacc caaatgaagc tataaaatct
     5761 gcaagagaag gcttttctat atggtctaat gttttagttc catcactact accatttata
     5821 attggagcaa acttaattgt tgatttaaaa atagtggata ttatagggtt tatcatcaat
     5881 cccataacta gatttgtatt taatgtttca ggaaagagcg ctttagcttt tgctatatct
     5941 acagtatctg gttatccagt aggagtaagt ttagcttctg aatttaggag taatggacaa
     6001 atttctaagt atgaagcaca aagacttgtg tctttttgtt ctacatcagg acctttattc
     6061 ataataggtt cggttggtac aggtatgttc caaaattcaa acttaggtta tttaatgatt
     6121 ctctgccatt atctaggaac tatattggtt ggtcttttct ttagaaatta tggtaatgaa
     6181 aatcttccaa agacaaaatt ggatttaaaa acaaatataa aaaatgtaat caacattaga
     6241 aattcaagtg ggaatggttt ttttgtttta tttggaaatg ctgtatttaa tggagtaaat
     6301 acattactta cagttggtgg atttgtaatt gttttttcag tagtatttaa aatactatct
     6361 ctttttaatg taatctcatt aatagcatca gttatatatt tgcctctttc atttttaggt
     6421 gttagtaaag aactctgtca agcatttgta agtggtctat ttgaaattac tataggatgc
     6481 aataaagtat ctagtataac aagttctcca gaaattttaa gagcatcttt agctagtttt
     6541 ttgataggat ttagtggatt atccatccta gctcaatgtt gtacatttct atcaaaaacg
     6601 gacatagatt taaaattata catactaagt aaatttgcgc atggagtttt agctgctatc
     6661 tttatattta tactttatcc aattgcaaac tctgctgtgt tggtttctag ctttgctgat
     6721 acatacaacg ttatatataa taatttaatc tggttttatt accttagcta ttatgataca
     6781 attcttcaaa ttgtagtaat aatttatcta atctcagccg tatttattgc aaaaaaacat
     6841 atgaaaactt tatctacaaa tgggaaacta atgcgatata aaaatacttt tttcactaag
     6901 aaattttcac gaaaaaaata aaactaaaaa taaaactaat aaaacttaac taaatacttc
     6961 cattttttta ttatatttgt cataatttta ataacctttt taattagttt aacagcatag
     7021 ttatttcatc acaatttaat aattttatta gaaatatttt tcaaaaaatt ctccttaatc
     7081 acatatatca cacttgtata agtttacttg taaatttttc cattatataa tatttcattt
     7141 atattatata ataaataccg acctaaactt accaaatcaa actaagagga gtggttgaaa
     7201 atggcaacaa gacctataga aatagacgaa tataaaaaga taatggaact attacatact
     7261 ggctttactt atagtgaaaa tggcgttgaa aaaagattta gaaaaaatcc taaagttgca
     7321 ttggcactaa tgttagaagc taatttaggt ctaagaatat cagatatttt aagattaaag
     7381 ataggtaatt ttaaaggtaa tatgttggaa ataaatgaaa aaaagacggg caaattacag
     7441 tatagaccag ttaataaaaa tataattgag tccataaatg aacatgcaag aaagtataat
     7501 cttaaatcaa atgactactt agtaaatata aagacaaagg ctattcaaaa acagttgagg
     7561 ataatttgta aacacttaaa tctgtataat ataagtacac atagctttag aaagctctat
     7621 gctactactc aatttgaaaa gagtaacaat aatttagagt tggttaaaga acttttaaat
     7681 cacagctctg tagccactac acagcgatat ataagggtaa ctcagcaagc tattaatgaa
     7741 gctagtgaaa atttctttat tggttaatta taacgtatac atttttctat tatcttatta
     7801 attcaccaga tattaaaaaa gagtgtctta aaatgaactt tttagttcat gagacactct
     7861 ttttttataa aatcaaatat atttttatta tttcaacact ggaaaaaagg cttatctctc
     7921 tattttgaga caccctatta aattttcata attcttgcat aaacatatta atctatcttc
     7981 aataattttt tagctgattc actgtaatta tttcttttta caatttccaa aactctatca
     8041 atctcatgtt ctgactttgc acttacaaaa atcgccaatt cttcatcatc tataatatca
     8101 taactatcta catatttatc taaacattct aaaattccat ctttattatt atacccacca
     8161 aatctaacta ctgcactaca ctcatcttct ataagtccac ttaaatccaa tttccctttt
     8221 gaaaaagact ttatatttga acttctatta tctattatat ctataatatc tgcataaaca
     8281 gcacttcctg ttgcacctct tccagctccc ttacctacaa aagaaagttc acctactgaa
     8341 tcacctacta aaataactac gttgaactca ttatctatct ttgaaagcat attattattg
     8401 tccactaatg ctggtctaac aaatccactt acttctcctg attcatactt acttctagca
     8461 accaatttta tcttacaatt taattttttt gcatattcta tatctttttc atctattgaa
     8521 gttattcctt ctaataataa atctttccaa tatactattt ttccataaga taaagtagac
     8581 aatatagata actttctagc actatcatat cctaaaacat ctgcatctgg attaacttct
     8641 gcaaatccca actcttgtgc ctgttttaaa gcaacatcat atggtaaatt ttcatcatac
     8701 atctttgata atataaaatt acaagtccca tttaaaatag cgtttatgct ctttatattg
     8761 ttgccttcca gtgattctat caatggtttt aaaactggaa taccacctgc cacagatgct
     8821 tcaaacttga tgctaacatt atttaagtta gctaattcta taaggtcact acctttttca
     8881 gccagtaaat ctttattagc tgtcactaca tgtatttttt tatttaacgc tcttgttaca
     8941 taatcaaaag ctggatttaa tcctccaatt acttcaacta gtatatcaat atccttgtca
     9001 aatatatctt ctatatcagt agttatctta tcaaaatact tcttatactt gtgtttttct
     9061 ttatttttaa ctaaaattcc aacaacttct atatctcttt ttgaagtatt attttctatt
     9121 aaatcaatta atccacttcc tactgttcca taccctaata ttgctatctt cactgtaaca
     9181 tacccccatc attttgatta tattaaatta tttatatata taaatatacc aataaaaaaa
     9241 gcttgagtat aaactcaagc catatgacgg gtttatactc atctttcagc ataaatgctg
     9301 caggatttag caccaatgta taattaaata tactggttgc cgggcttcac agggccagtc
     9361 cctctgccac tcttgataag attgctaatt taaatttatg tttaatagta taatacaatt
     9421 tcttccaatt gtcactattt ttttaactta tttccctaaa aggatataaa ctattatcta
     9481 acagctcttt tagttggttt accctttttt gtaacattat tatttttagt ctttgccttc
     9541 gcctgttcat gtcctggaaa aagtatttta ggctcttcaa tattcctctt gtaaatagta
     9601 aatttaaaac caatagcctg cacaaattca gctcttaaag cttcacatac tgcattagca
     9661 gtttctttag tatcaagacc tgcattttct aatatagtaa cttttactaa ttctctagtt
     9721 cttaacatac catctaattg ctctaagaaa ctctctgtaa caccttcttt accaatttgt
     9781 gtagtaggct ttaaagtatt tgccaaagat cttaaatacg ccctttgttt tccttttaac
     9841 atattctcac ctctataatt ctatacttaa attatctata atctatattt aaattaattg
     9901 taaaactcaa attctaattc ataaatttta accacatctc catcttctat tcccatttct
     9961 ctaagcctat caaaaacacc ttgactctcc atagcttttt ggaaatattg aagggattcc
    10021 atatcatcaa aatttacaga atacattatt cttctaagtg ctttacctgt aactacatac
    10081 acaccatctt caatatctat acttaactct tcttcagtac caatatcaag ttctggtcta
    10141 tacatttctt cttctgaaac tagctccaca tcttctactt cttttagcat tttagaaaca
    10201 taggctatta cttcatctat gccttgacga gttgctgctg acattttaaa tactgtatat
    10261 cctctgcctt ctaattcact cttaaacttc tcaaacttac tctcatcttc taatatatca
    10321 aatttatttg caactacaac ttgtggtctc tttgaaagct tttcattgta caatttaagc
    10381 tcatcattta tcttgtcaaa atcttcaata gggtctcttc cctctaatcc agatatatct
    10441 acaatatgta tcaatacctt tgttctctca acatgtctta agaactcatg acctaatcct
    10501 atgccttcag aagcaccctc tataattcca ggtatatctg caagtacaaa actatctcca
    10561 aacttagttt gaacaacacc tagatttggt gtaagtgttg taaaatgata gtttgcaatt
    10621 ttgggtttag ctttagttac aactgataga aatgttgatt tacctacatt tggaaatcct
    10681 aaaagaccaa catctgctat cattttaagt tcaagagtta tccatctttc ttctccatct
    10741 gtaccagatt ttgcaaaagc tggagcttgt cttactgcat ttgcaaagtg ttggttacct
    10801 ttaccaccta taccaccttt tgcaactata gctgtatctc cttctttttt taaatctgct
    10861 aatacaagac ctgtagcctc atctcttatt actgtacctg ctggtacttt taatactaaa
    10921 ttttctccat ttttaccagc tctttttcct ttgcttccgt cgccaccatt ttgagcttga
    10981 tattttttct ggtacttaaa gtccattaga gttctaagcc ccaaatcaac ttcaaatatg
    11041 atgcttgctc cacgaccacc atctccacca tctggaccac cagctggaac gtatttttct
    11101 cttctaaatg ctaccgaacc atttccacca tttccagcct taacaaatat tcttgcctta
    11161 tctataaaca aattcttcac ctacttaatt aaaaactttt aattagcatt accaaaaata
    11221 ccattataaa ttcatcagta ttataagaaa aaggagtgcc tcaacactcc ttttatgtat
    11281 attactcagc tattgctact ggatatatac taactttttt tctcttcttg tcttttcttt
    11341 cgaatttaac agtaccatca actaatgcaa ataaagtatc atcactacct tttccaacat
    11401 tagttcctgg atgtatttta gttcctcttt gtctaactat tatacttcca gcagttacta
    11461 attgaccatc gaatctctta acacctaatc ttttagatat agaatctcta ccatttttag
    11521 aagaacctac ccctttttta gatgctaata attgtaaatt catgtttaac atatggagtc
    11581 accccctact tttttaaaat ttttatattt tttgggtatt cccgtttaat ttctccaacc
    11641 gctaattgaa agtgatttag taataattgt gcgtctttta catgtgtatc tttgactata
    11701 cactctatat gaccttcttc tcctactact ttttcaaatt caatcttttg taatttgtct
    11761 aaagaattta taactgcaat agcattagat gtaactgcag cacatactat atcatagcct
    11821 atttcagcaa aatcagcatg tccctttaaa caaaaacctt gcaacaagaa atcatcatta
    11881 taataatatt taacttttat cataacttaa ccaataacta agcgtttatt ttttctacaa
    11941 ctatcttagt gtatgattga cgatgtcctt gttttcttct gtaatctttt tttgctttat
    12001 acttgaaaac tataactttc ttagctttac cttgttcaac aactttagct tgaacagatg
    12061 ctccttctac tactggagaa cctaatttta attctccatc tttagagcaa gctaatactt
    12121 cgtttaaagt aacaacatca cctgcgttag cttctaattt ttcaacgaat aatacatcac
    12181 cttcagaaac tttatattgc tttccaccag tctttactat agcgtacatt aaatacacct
    12241 cctcggcctc aaactcgcca gtacaaggta cttcatttga agatttaaac ctttactgtg
    12301 cggcattaca attattaatt ataaaatatt tacattttat tgtcaactat tttatcgaaa
    12361 ataacattgg ttttattgtg ctttaatgta ctatttgcgt ttaaagaaat ctttatatta
    12421 tatttttcac caattttatc aattatatcc atataatatt cttttatttg ttcaaaaata
    12481 aaatcattaa attctaccat tatgtttttg taaacagtat gttcacttat tctcataatt
    12541 tctttttcaa tactatctag tatataatgt acagatctta ttgtttgttc accatcacaa
    12601 catggacaag acattaaata atacttatca atggattctt tttctctacg cctagcaact
    12661 tctactaaac caagttttgt catacctaat acttcagctt ttcttttatc tttattaaat
    12721 tcttcttcta ggatgtgtaa aatctccttt tgatgcttct ttttatgcat atcaataaaa
    12781 tcgattatta ttatcccagc tatgtctcta attctaagct gtcttacaat ttcttttgca
    12841 gcttcaagat tagttttata aacggtttca tcaagtttac cagttccaat aaacttaccc
    12901 gtatttacat ctataaccgt aagagcttct gttttttcaa ttattaaata acctccactc
    12961 tttaaccata cttttttatt taataatttt tctattttag attctattct gtataaatca
    13021 aatacatctc tattttcctc aagacacagt ttatcaacat aagtcttatc tatacctctt
    13081 aaaatagact taagttcaga gtacttatca taactattgg taattataga ttcaatatca
    13141 tcattcacat tatcttttac atatttagtt gcaaaatcta aagacttata taataatttc
    13201 ggacctatac caagtttata ttcttttaaa atgttttcat actttaattt caactcttct
    13261 atatctttct ttatttcatc atgagtgcaa ccttgagcct cagtccttat tattaatcct
    13321 agattttcct tatttactgc cttagtaatt ttttttaatc taaatcgttc tttttcatca
    13381 gtaattttat ttgatattgt agttctatca ttggaaggta tatagacaat ataccttcca
    13441 gaaatactaa tctcagtgtt taattttgcg cctttagttc ctatttcttc tttatttatc
    13501 tgaaccaaaa tatcctgacc agattttata gtacttccct tttttaattg taaataagca
    13561 agtttcttaa atccaatatc tacaaaacaa gcttctattc ctggtataac tttttttacg
    13621 attccacgat atatattgga tacagtcttc ttatttaaat tgtcttcaac aaaaagttct
    13681 gttaatcttt catcttcaag tactgctgtt ttctgggacc ctattaggga ctcaataact
    13741 atctttttca caacttcacc tctcgagctt acataggagt cactaattcc ccatcttcta
    13801 atacatataa atctcttctc aatatatcaa catctaatgg gtcaatatca agttcaaaca
    13861 tctctaatat ctttggtata agtatattag tatttaaatt agtttttgat cctgtagcta
    13921 tagtagcagt aagagtaata tgttcatctt ctaaatttaa aacatcaaag gttcttatca
    13981 taggtcttat atctacttca accatcttac cttttttatt cttttttgtt atgattattt
    14041 ctttattatt cataaagtca attacttttc tttttacaaa ctctttcgtc ataggtcttt
    14101 tcaaatctat attaaacaaa tattctccat agtctatcac agaagaaaga gaaggtgttt
    14161 gtctatcaat ttcttttgct ttgataaaat ctatcccatc tggaagttga attgatactt
    14221 tatttaaaaa ttcctctact gataaatcat cttcttctat ttcaatatct acatactcac
    14281 cttgactttc tgtccctaaa gccaaggcat tgccataact cattttaggg tgaggattaa
    14341 atccttgaga atgtgataag tttatttcag ctcttctaaa cgctctttgt aacaatctct
    14401 gaaggtcaag atgtgatata tatatcatgt cgccttcctt tttaaatttt actcttatta
    14461 tcttactcat attagcaaag cccccttcct atatcatgta tatttattcc acaaccattg
    14521 cagttatgtc tacaatcaga agtgattttt tcttttttag cattttcgtc ttctctaatt
    14581 aagaattttt tactaatacc tacatctata tggtcccaag gaaatacctc ttcatagttt
    14641 ctatttctat gtgcataaaa atctatagaa agattattct tttccatagc ttctttccat
    14701 atatctaaat taaaatgttc tgcccatcca tcaaatttag cacctagtct aaatgcatca
    14761 tatataactt ttcctattct tctatcgcct ctagctacta ctgcttccat caaactagtc
    14821 tttgaatcat gataattgta ttttatattg ttgtttctta atttatttac taaatgtctc
    14881 tgcttattaa taacttcttc tgtagtatct tgaccatgcc attggaaagg tgtaaatggc
    14941 tttggtacaa atgtagaagt actcactgta acactaaaac ttctctttag ctttcctcca
    15001 tttacttttc tataaatatc aataacttta taagctaatt tagctatacc atctaaatct
    15061 tcataagtct cagttggtaa accaatcata aaatacaact taacactatt ccaccccatc
    15121 tcaaaagctc tttctgttgc attttccaaa tcttcttctg taacaccttt atttataaca
    15181 tctctaagtc tttgagttcc tgcttctgga gcaaaagtaa gtccagattt tcttacttgt
    15241 tgtattttat cagcaatttc cattgaaaaa ttatctaatc taagggatgg aagcgaaatc
    15301 ccaatattat tagatgcata ttcatctacc aaataatcag tcaactctga taacttacta
    15361 taatcactcg tactaagtga tgataatgat atttcatcat atccagtatt cttaactaat
    15421 ttatctaata tttctttaag tcttttaaca ctcttttctc ttattggtct gtatatcata
    15481 cctgcttgac agaatctaca tcctctagta catcctctaa ataattctag tactattcta
    15541 tcatgaacag tatctatata aggtactatt agtttttctg gacaatctac agtctcaaca
    15601 tcttttatta tccttttagt aggattttta gtaatccctt ctctattagg agttacactt
    15661 tgaactgtac catcttcatt gtacttgaca tcataaaaac ttggtatgta tactccttct
    15721 atgctagata ttttatataa aaaatcttct ctagtcgttt tatttttctt ccattcttta
    15781 tactcattta ctacttctaa attaacctct tcaccttcac ctaatattac tacatctaca
    15841 aaatctgcta atggttcgga attataagca caaggaccac caaccattat aaatgggtct
    15901 tccaaagttc tttcttcttt taatattggt atattggcta aatctaatat attcaagata
    15961 ttagtgtatg aaagttcata ttgaagtgta aaagtgacaa aatcaaagtt tgttataggc
    16021 tctcttgact ctaatgcaaa taatggtata cttttttctc tcattatatt ttccatatca
    16081 actgcaggag aatacactct ctcacaaaat acatcttcat ccttatttat tacatcatat
    16141 aatatatgac tccctaagtg actcatacca acttcatata aatctggaaa gcaatgtgca
    16201 tatcttatca attcactatt actagtatct ttatgaattg aatttatttc atttccaaga
    16261 tatctagcag gcttttctac tttttttaag atttttttca agtctacttt attcatttat
    16321 taaaattcct ccaaacaagc tctttatttt tattcttatt tataagtaat attatacacg
    16381 ataaaatatt ttctatcaaa aatataacta aaatattgaa tttaaaatac atattattca
    16441 caaaatagaa tagctaaaaa tatttaagaa tattttctat taaattttta ttgaaataca
    16501 atttttttac aaagtatgat atattataaa cataaaacta ttgttttaca tgacttatca
    16561 tgacatcttt tgtttcattc acctcaaaat ggggtatgtt acttatataa tgataaattt
    16621 taggagggtt aaaaatgagt aaagtttttg aagtaaaaaa agatatctac tttactggag
    16681 tagtagatga aggtttaaaa gtattcgaca taataatgga aactgagttt gggacaactt
    16741 acaactctta tttaatcaag gacgaaaaaa cggttttatt tgacactgta aaggcaaatt
    16801 ttaaagatga gtttttaagt aatttatcag aggttactga tatagctaaa attgactatg
    16861 ttgtaattca tcacacagaa ccagaccatg caggtagttt aaaatactta ttagatataa
    16921 atcctaatat agaagtttat tgtactaaag ctgctaaact atacttagat ggtcaaataa
    16981 atagaccttt taactgtcat gtaataaaag atggtgaaat tttaaatatc ggtaaaagaa
    17041 atcttagatt tataactgct ccattcttac attgggttga tacaatgttt acttacattg
    17101 aggaggacaa gactctttta acttgtgatg catttggttg tcactttgca agtgttgatg
    17161 cagaggtagt gaatagtgaa gattatctaa aatcagctaa acactactat gattgtatag
    17221 ttaagccatt tgctaaacat gttttaagtg ctgtagataa ggtggtagga ctaaacatag
    17281 aatttgatac tatattgact tctcatggtc caatgcttac taaagaccca atggctgctg
    17341 ttaaaagata tgttgagtgg tctacagaag ctgttaatac aactaatcaa aatcaagttt
    17401 ctatattcta cttatcagct tatagtaata ctttagagat ggctaaaaag ataaaagaag
    17461 gacttgataa agaaggtgca aaggctgaat tatatgattt agaagatatg acattaacag
    17521 aaatgcacga tacattagta gtgtcaaaag taatattact aggctctcca actataaaca
    17581 aaactatggt taaacctatg tgggatttat tctctgttat agacccaatg gctaaccaag
    17641 gcaagatagc aggagtattt ggttcatttg gttggagtgg tgaaggtata actatggctg
    17701 agactttact taaatctatg tcattcaaaa tgcctgtaga atctcttaag aaaaaattct
    17761 tccctagtga agagacatta aaagagtgta tggcatttgg tgcagaattt gcaaaattag
    17821 ttaaatagcc attaatcaca cataatatta tttaaaataa atgattttat atttaaaaat
    17881 aaattaaaat ggagttgtct caaaattaat ttttctaatc taattttaag ttcaactcct
    17941 tttttatttt ctaaaatgac tgatataatg ataaatgcac cctttgtgcg agggtgcatt
    18001 atttataata tgttctattt gtacactagt atatttgtat taattcacat atctagcgta
    18061 tgctaccatc cctcttccag aattagatgc cgtttcacca atttccactt taataattac
    18121 attattttct ttacactttt ttgataattc tgctgcagaa aggttcttac cagtaaaatc
    18181 taaagaaaat ggcttatcat attcaaaagg atactcatca ggtacaccat ctggagctaa
    18241 tgcaaccata ccatatggtg tattcataaa ccctattaca gtaactgtat gctttgatgg
    18301 gtctaaattt gaatttttgc ttgtaaactt aattgtattt attgatgagt tgattaaact
    18361 acacggaact ccctcataca tttcaatttt tggacgtaga ttatttccat aaacttcatc
    18421 aagtattgcc ccttgttgat ctgctatcat agtgtgattc ccaggtccaa ctggcgcaga
    18481 aggcttagct ggcgtaggtt tcttaacagt tggcgttgga ttctgtgtct ttactacttc
    18541 gccaacagct ggatatgctt tcttgtcaaa tggtaatggg tgagtaagac tcttgtatat
    18601 gatgtctgct cctgctgatt tgttggcatc aatatctaca tgatacaaac gtccatcctc
    18661 aaaacgaata agattccact gatggtcatt tgttgtacta tgtacataat aacattcaat
    18721 tcccatagca tcacataaag ctttcaagct cctacacatt ccagaacaag cacctttttt
    18781 agtaaccaat gtataagcag tattggctcc ctctgctgcg ttccagttaa catatgttgt
    18841 gttgttttta atgtgattat aagcaaccct aatcttagca tctgtggtca tgttctttgt
    18901 aattttcttg ttcataaagt ctgctgttgc ttgtgctacc gaattaagtt gagcttgtga
    18961 taactttgga ccatatatac tgttggttcc agctacataa cttttcggtg tctttgcagt
    19021 aacatcttta gctgtcatac cataaactgt tacactcata catgaggtta aaaccatcgt
    19081 tgcaatcaat agtttagtag ctaatttcct gatttttttc atattagcat tctcctttat
    19141 aatatatttt actttcaatt ccctaccatt aattaagttc attttagatt taaaatatgg
    19201 attatcaaca tgtatgcata gcaattcacg tggtcataga atatttagat ttcttggaaa
    19261 atctgttcta tatttcctat atagttcgta tacaatccaa ctcacaaacc tgcatttttg
    19321 atatcatcac aataaaatga taataattaa tatattcaaa aatgttggtc tgtaacaggt
    19381 atgtcaattt tccaattatt ggtaatggcg agagtgagga gaattgtcaa taatacattt
    19441 aaaattaaaa ttgtatacac ttcatacatt ctcccctaac aacattatac agattataat
    19501 ttgtatattc aatacatttt ttggaaagta taattatatc aaaatatgtg attatgtata
    19561 gatacaatcc actatggaga aatataaaag aaaagagtat aacaaacaat aaaaaataag
    19621 atgattcaaa ttttattttg aaacatctta tttactctac aaattaagat tatttggtga
    19681 attatttaat tattattttt tattaaagaa attttaaatc ttaatataaa atacgctatt
    19741 atacataata atccaaatgc aaatgctact ggaattaaca tctccgattc aaaataaaag
    19801 aaatattcta agaaaatatc tggatttact atattcccat tcttgtctaa aaaatctttt
    19861 ccattatatc ctaaatgctg tataaaatta cttatgttat tataatctgc ttccctctca
    19921 tataccatat tagcattcaa tccagaagaa cttaaattag tgtttccatc tttcatatct
    19981 attacatata gtctaagagc tttgacttta ctatacttat ctttatcaag tattttttca
    20041 agttctaact tatattcacc aatctttgtc atatcatgtc tttctgccaa ttgctttatt
    20101 tctccatcaa gttcttttcc taaattatac atattatgtt gtaaactatt attgtaatac
    20161 cacataaatc cagaaattaa aaatgcaatt atacttatgt aacctaatac ttttttaaac
    20221 cccattacaa ttgatttatg cctatccaat gataaagata gttcttttat tatattacat
    20281 aattcatact gcatttcatc accatcatca aatctttcaa ttgctttttt acatgcttct
    20341 tcttcatcta atccttctaa tttaaattca tttgcagatt ctataagatg gcatctcatt
    20401 tcctgtttta attctatagt acttttattg tcacattttt tatatagttt atctaaatat
    20461 tcatctatta gtttcataaa ttaatccacc tctcccaaaa attgattcat aaccttttta
    20521 ataaatctcc attcttgttt ctttatttct aaaaaatctt taccttcatt agttaaatta
    20581 taatattttc ttcttccacc tgaactttgt tcattgctat aataagattt aattaaattt
    20641 ttagattcca ttcttttaag tgcaagatac attgttcctt cttttaattc aaactcactt
    20701 ctttctctta cttgctttgc aatttcatat ccataacaat ctttcttttc taatatagaa
    20761 acaattaata tatcgatata accttttaac acttctttat ttaactgcat tatgtaccac
    20821 cgccttttca cctactactc tatattgtat agtacttata tttactataa taatacatag
    20881 tactctataa tgcaaggttt atattctaca atagacttaa aaataataaa tagctgtata
    20941 ttaatattta atctataaat actaatatac agctatttat caaattttaa aaataaattt
    21001 ttgttttatt aatgttgtca taacaacatt tacttggttg aaatagtatt acctataagt
    21061 ttaaatatat ataataatag acttgattct atttcatatt attaaaatag agtaatagtt
    21121 aaattctact actctatcta taatctattt cctaattatt taatctcaaa tttagctctt
    21181 catcatttaa atctttcata agatttctac aggtactaga tttattccaa cagaaaaact
    21241 agggcattaa acacttatat atctgtttct ttagactgta atattaatat tttttaaata
    21301 cgttgacttt tgctttttct aacttctctc catgatagtt ctcctctatc aagagctttt
    21361 ataaatattt ctgctgaagc tagatttgta gctattggaa catgataaac atcacaaagt
    21421 cttattaacg cttgtatatc tgtttcatga gcctgagatg tcaatgggtc tcttaaaaaa
    21481 ataactaaat ctatttcttg tgttactatt aaagaaccta tttgttggtc tccacctaaa
    21541 ggaccagatg ctaatctatt tatatttaat tcagtttcat ccattattct ttttcctgta
    21601 gttccagttg catatagtcc atactttttt aaaattgatt cataacctat acaaaatccg
    21661 accatagtgt ttttcatttg gtcatgtgct actaatgcta tattcatata tctaatctcc
    21721 tcaatttatg tttagatgta tttaaattta gaaatttatt ttctttcttc tcatacacac
    21781 atttagtact aatcctaagt ttgctaaaga tgtcaataac gaacttcctc cataacttac
    21841 aaaaggtaat gttactccag ttactggtat taacgcaacc gtcataccta tattttggat
    21901 tatctggtaa ccaaacattc ccataacacc aaccactatg agggttccat aaaaatcttt
    21961 tgcatctctt gcaattgcta aaagccttaa taggaatatc ataaataaga ttatcaacac
    22021 tgccattcct attacaccaa gttcttcacc tacaacagca aatataaaat cactatcttg
    22081 tacaggtaaa aaatcttctt gattttgact tccattgtaa agtcctttac cagttacgcc
    22141 tccagaacca attgcaatca gagattgcat aacttgatag tttcctttta gagttacatc
    22201 ttctggattt agaaaagctt ctattcttac cttttgatgg tctgccatca ttaaatacat
    22261 cagtggcatt gacactaata gtatgattat tcctcttttt atcaactttg agctaagacc
    22321 tgctgtgaac agcattgcaa atatcataca acaaaataca attcctgttc ccaagtctgg
    22381 ttgtgcaatc aataatccga taaatggaag agaataaact acaacaggca taacttcctt
    22441 taacgtattt aatttatcct tcttggattc aagtatcttt gcataactta gtacaaatgt
    22501 taatttgact atttcagatg tctgcaagtc aagtggtcct aagttaatcc acgacctagc
    22561 tccacctttt actgtcccaa ttccaggtaa taaaactatg gctaacaaaa taagacttat
    22621 tatataaagc gctttataat acctgcctag aaggttatag tcaaaaaata atatgactat
    22681 aatcattcct ataccaagta caaaagctaa tccttgttta atcaattgat tatatgaacc
    22741 tgttgaatta gcatgtgttg cactactaag tataacaagc ccaaaaataa agatagctaa
    22801 tactgtaact ataagtttcc agtctaactg ttttatcaac ttaattgtcg acttataatt
    22861 aatattgatt tttttcaaag aatcaccgtc tttctatctt taaacatata attaaataaa
    22921 aggggcatat accaataagg atatccccct tttaagcact aattacatac atctatcttt
    22981 tatattcttt agtggtatat tagcaactaa tgcagataca ggtttgtcat catgctcacc
    23041 tctgcattta gacattttta tttcaagacc atcatcttct atttctgcat aattagctat
    23101 tactttcaaa atgtctgttt ttatcatctc taaaagttgt ggtgaacaat ctactctatc
    23161 atgaactaaa actagcttta acctctcttt agcaacagat ttactagttt tagcctcgtt
    23221 agaaaaaact ctaaataaat ctaacacaga tgtatcctcc tttgctactt agccatgcta
    23281 aacatttttt taagtctacc aaagaatcca tcttcaactt caagatcaag tagaggaact
    23341 tcttcattta gtatcctttt tgcgatattt ttgtatgctt gaccagcaag tgacttagaa
    23401 tcaagtatag ctggctctcc tttatttgtt gatataatta tgctttcatc atcaggaaca
    23461 agacctaaca aatctattgc taatatttct attatatctt gtttatccat catgtctcca
    23521 cgcttaacca tatcatttct aattctattt ataactaatc ttatctcttt tatttcattt
    23581 gcttctaata gacctattat tctatcagca tctcttactg ctgatatttc tggattagta
    23641 actacaatag ctctatctgc cccagctact gcatttttaa agccttgctc aatacctgct
    23701 ggacaatcta ttattatata ctcgaaagat tcttttagtt tctcacacag gtcaatcatc
    23761 tgctcaactg agacagcatt tttatctcta gtttgtgctg ctggtaataa atatagatta
    23821 tcaaatcttt tgtcttttat tagagcttgc ttaagcctac aagttccttc aactacatct
    23881 actatgtcat aaactattcg attttcaaga cccattacta catctaagtt tctaagtcca
    23941 atatctgcat ccacaactac tgttttttta ttttctaggc ttagcgcagt tcctaagttt
    24001 gctgcggtag tagtttttcc aaccccacct ttaccagatg ttataactat aacttcgctc
    24061 ataatactac cccctatttt tctttctatt tgtctaactt tgacaaatag ctttcgatta
    24121 caattcttcc attacttaca aaggctattt ctgggctaat ttctgattca ctttcataat
    24181 tttcttcatc atctggagct tctgcaatat tatctgctat ttgtaatatt ttaggattaa
    24241 gatttccagc aacaacatat gcaaattcat tgccttctgc acctgcatgt acaaaacctt
    24301 ctattttacc cattacaaaa acattacctc ctgctacaac cttagcacca gcatttatat
    24361 ctcccataac aactgtattt gattttgaaa ggacttttga accaggtttc atatcattca
    24421 atataactac atcaccttca aattcaatat tttcaccaga tcttagggta ttgacatatt
    24481 ttgttggaaa attactattt tcttcaattt tttgatcttc aacaaattcc acatcaaatc
    24541 tagaagtaat tccttcttta agttctagaa tctccatgtc agttaaacaa tcactattaa
    24601 ttttactaat tttagcccca ttaaaaaagc caacatatgc ttccaatttg tttattattt
    24661 tttcctgtat ttcctcaaat ggagcttctc tttttatatt tactattatc cctcttttat
    24721 tacccttgaa ctctactagt tcttgactac aaatttctct taaagacata acaacctcca
    24781 gagtgatata tcatttatat tcttcaaata cgctttaaac ccttttattt tttctatata
    24841 aagctaaagt ttatacatag attgagctat ctattaattt gtgactcgaa atttatattt
    24901 tcgtcagaag aattgttaac atcattcgtc ttgttgtcat ttttactatt agttggctct
    24961 aatcccatat aagttccaat aacttctcta gttggtaaaa gtgagaatac acttgaatca
    25021 ccttgaggaa ttacacatac aacagctatt tctgggtcat ctgctggagc aaaagcaaca
    25081 gtccacgtaa aagaaccata atcttgttta aatctatcta tttggtcatc tgttattttt
    25141 ggatttaatt cttttatagc ttttcttaga tatgaagaat tcaccttatc agtgtcctct
    25201 aatctaactt ttactccatc ttctaattct tcctctaatt tctttctttc ttcgtctttc
    25261 aaatctttat tttctaattt cttttttatt tccttttcct tagctaatga aagctctttt
    25321 tctttttctg ctttcatctt atcagctagt ttaatcgcat catttaggtt gacattataa
    25381 gatgacatat gactttttaa atattcatac tcgttatctg taggtatttt cccacttttc
    25441 tcagctgtac cagtttttgc tgctacatct attggaaaat cagcaaaagc actcttcgct
    25501 gtaccttgtc ttgcaaccaa tttcatacct tttgttaatt cttttaagtt atctggattt
    25561 ttaaaaggta ttttttcgac tttcttctgg tcattaattt taactgatga ataatcactt
    25621 gaaacagctc tatcaactac agataattct actaaattac ctccatttgc tatagcagct
    25681 acatatcttg atatttgagc tggtgtatag gcattttcac cttgacctat agctaagtta
    25741 aatgtatctg cagtactcca ttttgcaaag ttaaaataac tgaatactgc taaatctgct
    25801 acttcttcta ttctatcttc tttaacattc attttcttta gcctattcat tgtttcgact
    25861 ctaccaggag ttttcttttc tgctgcccag cttacaatct cattgattct tttctcatat
    25921 tctttaggat ttttttctct agttatatca acaaaatcat ttgccatttc tctttctaaa
    25981 tttgacttta gaagagcttg tgttgatttc aatttatctt ctgtgctagg aactttacct
    26041 gctctttctt ctacttcatc ttgaagccct gtattttggt caagcccaaa taattttgca
    26101 tactcaagta ttttatttgc attcatatct ataccaatgc tttttccatc tggccatgtt
    26161 ttacctgtac caattgttgc catatatata ttacaagact cttgtattgc tttatataag
    26221 tttgtcatac catggtttcc tctaccatgg ttccatacat agtcaccaaa agatttttta
    26281 cctaacatta taaccccagg gtcatttata gtaaagtttg gatccagacc attttctaac
    26341 gctgccattg aagttaccat cttaaatgaa gaacctggct gaaattcccc ttgtgtaacc
    26401 aagtttaaaa gtggacttcc tgctagtaag tcatttggat tttttggttg aagtgcttta
    26461 taatcctctg tagaaatacc ttttgcaaat ttatttgggt cataattagg ataacttgat
    26521 gaagctaata cttctccatt tttaacatca atagctataa gagctgctga ctgtgctttt
    26581 cctgcatatg cactaattgg tttgtcccca aatttacttt taaaagtacc tcctttagaa
    26641 gcaacttcga ttatctgttt taatgcattg tctgaaactt cttgtaaatc tttatctagt
    26701 gtaagataca cagtatctcc tgatttaggt tttttagatt caatttcttt actgattcta
    26761 cccaatgcat caactttaac cattttataa ccatcagttc ctcttaatct actttcatta
    26821 ctcttttcta ctcctgcaag acctaccata tctccagttt cataaccttt ttgaaggtat
    26881 gattctattt gagtggatgg catttttcct acatatccta acatatgaga agctaatgac
    26941 ccattaggat aatatcttac tggttctact gctattgaaa caccaacaag gtccatagca
    27001 ctttcttcaa tttgagcaat cgtagtttct cccacatcct tagcaatagt tactggatta
    27061 tattgagaat atccttttga ttttatcaaa tcccttacga ccattatttt tcttgcatct
    27121 tcatcactca aacttttatc aatttctaat gcatcactat ttcttacttt ttcaaacgct
    27181 tctttggcac tcagtttagt ttcttttatc ttataactag ccaaccaatc tctcttatct
    27241 ctttctgctg tgaacatcca tttactaact aaaataggtg gataatatcc attttcattt
    27301 agttttgcct gtaatcttgt cgcatccaat cttttatctt cttgagataa tattccagct
    27361 gatataagtt tatccaccaa gtaataaaaa ctttccttag cattataatc atttggaata
    27421 ccattttctg atttatattc cctaatatct ctgtcatatg tataatagta ctttccattt
    27481 tctacatata taggaaactc atctacatat tcttcaccat ttctctccag caaatttata
    27541 agttttaatg atatttcatt tgctcttgaa tgcttatttg catctttctt attaatatcg
    27601 ttacctgaaa cttgaacagt aaataagttt ttatttcctg ccaacagtct tccatatcta
    27661 tcttttattt ctcctcttgg agcttctatt gccaactttt tatatgtttt attctcagct
    27721 aactcattgt aatattcata tttaaatgta gtcatataga ttatttttac taaaatagct
    27781 aaaaatatca cgcccatgac tatcttaatt atttttagtc tatcaacttg tttattttct
    27841 tccatgaaat caccaactaa tcttctttaa gttttaaaac tgctctttta gataagcgat
    27901 atattactaa accaattaaa ctattcacta ttggtgctac tagtagacct tttacaaaaa
    27961 gtgttgatat gttgtagctt tgataaatta tactagatgt acctatattg acaattgaat
    28021 caattaaact gcctataaaa attaaaacga aagtaataat ataactacct ttatatagct
    28081 gatctctcat gtatccaatt gcatatgcaa taataaacaa aattaatgca tttacaccaa
    28141 aaattccacc aacggttatg tctttaataa gccctattat tgctgctata atcccacctt
    28201 ctaattcatc taaataaaga gagattatgg ttacatatat aagaactaaa ttaaagctta
    28261 tgccaaaaat atctatataa tttgttatac tattttctat agtcataagc acaatcccca
    28321 acagacaaag taaaactttt ttcattgttt ttatcccctt tatcctatat ttcttggttc
    28381 tatgactaca acgtcattta tattcttaaa atcaacataa ggttttacaa ctacatattt
    28441 taatgactta tctttatcat caaccacttt ttcaacttcg ccaattggta taccatctgg
    28501 gaagaatcca agacctgatg ttgttattat atctccttga attacattat atgatgagtc
    28561 aaacatatat ccttgcaaat atcctttact attataacta tctttattat ctaaagtagt
    28621 attttgagta attgtacctt tagcattggc atcctttgct agtttaaagc ttacagatgc
    28681 cttcgaatcc aatagtgaaa ttgccttact ataattgttg gaaacctcat aaactatgcc
    28741 aactaatcca ctaccattca taactatact ttcttttttt acacctgaat tttttcctgc
    28801 acctatgaca aaactctcat accagtttcc atcattttta ccaacaacac ttgctgaaat
    28861 acttgtcgcc ttatactctt cttgtacaaa gttcagtgtc ttttttaact cttctagcga
    28921 ttctgtttta tctagtttgg catttaaagc tatcatattt tttttaagtt tttcattttc
    28981 ttcttctaat ttttttacct ttttagaatt atctttaaaa tttgctacat ctttaaaacc
    29041 atttttgata aaagaaaatc catcatttat tcccttacct acacttgtaa tagcatctga
    29101 agctactcct aaatttactg gactaccact agaaaattta ccaattgata tacccacaat
    29161 tccaattaaa gtgatagcaa ctacgcccgt tgctatcact ttaacattaa tccttttttt
    29221 ctcatttttg ttcttgtcaa atctcaaggc catcaccaac ttctaattct tagtattcat
    29281 cattaatact ttttcaaaga tttcttggtc ttcaacagat ttacctgttc caagtgcaac
    29341 acaatcaaga ggtgtttctg ctatttgtac agacatacca gtttcctgtg taataagctt
    29401 atcaagacct ctaagcaaag cgcctcctcc tgttagcatt attccgtttt ccattatatc
    29461 tgaggctaat tctggtggag ttctttctag tgtagatttt attgcatcta ctattgaact
    29521 tacaggttct tttaatgctt ctctaacttc tgtagagcat atttctattg ttttaggtaa
    29581 tcctgagatt aaatctctac ctcttatttg catatttatt tcttcatcat ctttaaatgt
    29641 agaacctatg ttaattttta cattttcagc agttctttca cctatcataa gattatattc
    29701 tttttttaca taagccacta tagactcgtc aaattcgtct ccacctattc ttatagattt
    29761 agcagtaacg attcctccta aagatattac agctatttct gaagtacctc caccaatatc
    29821 aactaccata ttaccttctg gttcttcaac ttttaatcca gcccctatag ccgctgccat
    29881 tggctcttct ataaggaatg catctttagc tcctgcttgt cttgcagcct cttctatagc
    29941 tctcttttct acttctgtaa ctccaaaagg aacacatata gctattcttg gacttacaac
    30001 acctttttta tcagccgctt tttgtataaa atagctaatc attgattgag ttatgtcaaa
    30061 atcagctatt actccatctt tcataggtct tatagctact atatttcctg gtgttctacc
    30121 tatcattttt ttggcttctt cacctactgc taatacctct ttactatcat ctcttattgc
    30181 gacaactgat ggttctctaa caacaatacc ttgtcctttt atgtaaacta atgtatttgc
    30241 agtccctaaa tctattccca tatcctttgt catcttatta aaactaaaaa aacctttttt
    30301 ttctttcttt ttttccttag ccatgatttt actccttcct actattaaga atcatgttta
    30361 aaacattctc ctattatttt atcacatata aagtcaaatt atcatatttt ctttaaagct
    30421 aaagtataat ccatctccaa taattatatg gtctattatt tctattccaa ttatttctcc
    30481 acatttaata agtcttgatg tgatattttt atcttctctt gatggttcta cacttcctga
    30541 agggtgatta tgcatcacta ttatcttact tgcacttctt cttatagctt ctttaaaaac
    30601 ctctcttgga tggactaatg acgagcttaa agtccctata gatacatcta catcagatat
    30661 tatctcattt tttgtgttta aaagaaccgc tttaaaaacc tcttttttta aataccttag
    30721 gctatccata taatacctct gtatatccca aggattcctt attttatact ttataggttt
    30781 aaaactagct actctagagc ctagttctaa agctgcttta atttgggttg attttgataa
    30841 cccaattcca tctatattac acagctcctc tattgtcata tcttctaaat gtctaatacc
    30901 ctgaatatct ctatttatta tatagttggc taattcaata gcatttttat tcttgttgcc
    30961 agttcttaaa agtatagcca acaactcagc atttgataag ctttttacgc ctttggcaag
    31021 catcttttct ctgggtcttt cctctggtgc catttctttt accttaatgg ttgaattaaa
    31081 tgactttttc agataaatca cactccataa aaaagattta tactaaaata tttttttaat
    31141 aggtcgccta accttgaaat tggaagacca actatattaa aataatctcc tctaatttct
    31201 tcaactaaca aagctccata accctgtata ccataggctc ctgctttgtc aaatgactca
    31261 ttagttttca gataatcctc aatatcctgc tctgataacg ttttaaattt cacattgctg
    31321 attacatagt caattacctt tttatcttcg cacaagttta ttaagctaat acctgttatg
    31381 acttgatgct ctcttccaga tagtcttttt aacatatctc ttgcacaaga ctcatctttt
    31441 ggttttccta aaatagcatt gtctaaaaca acaactgtat cagctccaat aacaacatct
    31501 tcattatgtt cagatgctac agacatactt ttttcaaaag ctaatctcat aactaaatgt
    31561 tttggtgttt caccttccaa tataatctca tcaatttcat ttttaattat atcaaatctt
    31621 acattcgtat tctctagaat ttctttcctt cttggagagg ctgatgccaa gattatattc
    31681 atttattcac ctcatttgta cgtaatttgt acctatttag tctatcacta tattgtaaaa
    31741 aattcaacag aaatgtcgaa ccttttttgt tacactattg ttatatttat taccattaga
    31801 ataaatatac ataaaataga ggtatggaaa ttattccata cctctgtttt atgtactcat
    31861 tagagatatg gaaattattc catatcctta ttttgtccat attacaaaat tttaatagct
    31921 ttctttggac atttttccat acataaatgg catcctacac atttatctgc atctacctta
    31981 tgtttttctt ttaattctcc ctctattgca tcaaacttac attgtttttt gcacactgta
    32041 catccaacac aaagttcttg gtcaatagta actttctttt tcttagttat gtcacctgat
    32101 attacttttg tagggcattt tgctacacat tgcatacacc ctacacactt attagggtct
    32161 atcttagcta ttttgtcttc aaatattatt gcatcaaatt tacaagcttt tacacacatt
    32221 ccacagccaa tacatccagc agtacatttt tccttaacag ctttaccaaa ctctttgcta
    32281 ttacactcta caactacttc ttgactttct ggttttgtga ttattatacc cttaggacaa
    32341 acttctttac attttccaca atttacacac ttttcttcat ctattactgc tattccatca
    32401 actatagaaa tagcatcaaa tttacaagca tctttacatg ttccaagacc taaacatcca
    32461 aatttacaca tcttagcacc tgagtttaaa acatttgctg ctctacaatc tgatattcct
    32521 tcatactcat atttatcttt tgcacttgaa caagttcctt tacaaataac ttttgctact
    32581 tgtttctcac ctgcttcagc acttattccc attatttctc caacttttgc accaacatct
    32641 gctccaccta cagggcaacc attaactggt gcattgcctt caactatcgc atttgcaaga
    32701 cctccacaac ctggaaatcc acaacctcca cagttggctc ctggaagtac tcctagtata
    32761 gcttccactc tctcgtctac ttctacagca aactttttag atgcaaaatc tagtactatc
    32821 ccgaagatta agcccataat tcctaaaact aatacagctg taagtatcac cattttcacc
    32881 cctctttttt atagctttat aagtcctgta aatcctaaga aagctattga cattaaacct
    32941 gctgatataa gcgttatagg gaaaccttta aatgcttctg gtatatcagc taattctagt
    33001 ctttctctta tacctgcgaa tattactatt gctaaagtaa atccagctcc ggctccaaat
    33061 ccgtttatta ttgtttctac taaattataa cttttttgaa cattaactag agctatacca
    33121 agaactgcac agtttgtagt tataagaggt aaataaactc ctaatgcttg atataaagat
    33181 ggactcatct tttgaattac catttcaacg aactgaacta tagaagctat aaccaatata
    33241 aaagctattg tttgtaagaa tccagttcca gtttttacta ataaaatctg tataaaataa
    33301 gttataattg aagctagagt taaaacgaac gtaactgcaa ctcccatccc tactgctgta
    33361 tcaaccttct tagaaactcc caagaaagga catataccca agaattgaga tgttataacg
    33421 ttattaacaa gaacgatact taaaaataaa agtattaaat tcatttatta cacctccact
    33481 atgctttttt acttcttaat ttattaaatc ctgcaaataa gaatcctaat gttaagaatg
    33541 cacctggtgg taatataaat attaatactg gttggaaaga tgctccaaat aatgtcattc
    33601 caaataaact accatttcca agtaattctc ttactgcacc tattattgta agtgcaacag
    33661 taaatccaag accttgacct acaccatcaa ctattgaagg catagctcct gttttgaaag
    33721 caaaactttc tgcacgagct aatattaaac agttaacaac tattaatggt atatatatac
    33781 caagagcctt atcaagagca ggaacatatg cttttaaaag catacctact attgtaacaa
    33841 atgtagctat aactactata aatataggta ttcttatctt atctggtgta atttttctta
    33901 ttaaagatat aactaaattt gcacatatta aaactgctgc tgttgaaaga cccataccaa
    33961 ttccatttat tgctgaagtt gtaacggcta gtgttggaca catacctata acttgaacaa
    34021 atgttgggtt ctcatctatt agcccatttt taaatacttt agctaaattc ataattaacc
    34081 ctccttattt attaagagca ctattgaaaa catctatagc agcatttact cctgtagtaa
    34141 ctgctgtaga tgtaatagtt gcacctgata ttgctaatat ttgattatct gctgttgtac
    34201 ttccttttgc tacttcaaga ggctcagcag tttttccttt gtattgaccc ttaaatgcat
    34261 catccttagc ttttgctcca agacctgctg tttctgacat attaccaatg tcaacacctg
    34321 taacttgacc ttcagatgat atacctacca taacttcaat ttctccacca tatccacttg
    34381 gtttagcttt taaagtatat ccaaccactt cagaaccatc taaaccttca tacacttcag
    34441 ctataagatc atctccaagg cctttgtaag tatcttcctt catttcttta aattcttttg
    34501 cttctggaag gactgctttt ctaagttcat tatttgcttg tatatttctt tgttctatta
    34561 ctggagctgt aacctgatta gtagctccaa gagctaatgc agctattgca cttatagcaa
    34621 gtaaagtacc acctaatctc accatactgt tcattattta gccacctccc caaatactct
    34681 atttctaaca aacttatcta taagtggagc aagacagttc actaatagta ttgagtaaga
    34741 tacaccttct ggatatccac catacattct tataacagtt gttataagac cagctaatac
    34801 agcgtatatt atttgacctt ttttagtcat aggcgaagtt gtataatccg taagcatgaa
    34861 gaatccacct aacataagtc ctccagcaaa cagttgatat attgcgaaag ttatattaaa
    34921 tccacctaaa aggaatgtta atataaatac agttgctatg taaaatactg gtataacata
    34981 atttattatg cctttataca ttaggtatac tccacctaat aataataata ttgctgatgt
    35041 ctcacctata catccaccaa tattccctat catcatgtca gcaagactaa ttccattgct
    35101 agaaagatct gccagtcctg ctgctccatt tttcaaaaag cttaatggag ttgctgtagt
    35161 aactgcatct aaatttttac ctatccagtt aacacctggt tgagtccaag cagtcattgc
    35221 tactgggaat gatgctaata aaaatgctct tgcagcaagt gctggattga caaagttaca
    35281 tccaattcca ccaaatacca ttttaactac taatattgca aatattcctc ctactagaca
    35341 catccaccaa ggaagtgatg ctggtacgtt aaatgctaat aataatcctg ttacaacagc
    35401 tgaaaaatct cctatagtac ttttttgctt cgtaaatttt tgatataaaa attcagtacc
    35461 tactgtacca agtatacaaa aaaacatagc actcaaagca ttaagtctaa agaagtatac
    35521 tcctgctact gctgctggaa ggagtgctat aataacttgt ttcattatat atgaagtatc
    35581 ttcattactt ctcacatgag gagaagatga tactatcaac ttattttcca tcgtttttcc
    35641 cccttaactt tctttatttc tgagcagctt gctttcttct ctttgctcct atttctctct
    35701 ttgctgatct tatagattga agtaatggtc ttcttgctgg acaaataaat gagcaagacc
    35761 cacattctat acaatccatt gctctatgat attcagctgt gtcatagtca tcttttaaag
    35821 aatatgcact tatgaaaagt ggttgtaaga atgatggaca tacatctgta catcttccac
    35881 atcttaaaca gttttgaata tctggagtac gtgattcttc ctcatccaaa cataaaattc
    35941 ctgaagaacc tttgttagta gctatttcag tagtatattg agctattccc atcataggtc
    36001 cacccattat aaccttacca atcttcttat cttctttaaa tccaccacat tgttctatta
    36061 tttctgaaac taatgttcca acttttgtta ttaggttctt tggctctttg atgcaactac
    36121 ctgttattgt agtgattctt tcaaccaaag gcatacctgt tttaatagac ttagctatct
    36181 gtgctgctgt ggcaacgttg tcaacaactg cacctgctgc tattggtaat ccaccagatg
    36241 gaacttcttt gcctgtacac gcataaataa gttgtttctc tgctccttgt ggatatttca
    36301 cttgaaggcc aacaacttcg atttcgctat agtctttagc tacattttga attgcttcta
    36361 tagcatctgg tttattcgtt tcaattccta taaatccttt tttgacatct aataccttca
    36421 ttaatgctct taaaccaaac acaacatctt ctggattttc aaccattaat ctgtggtctg
    36481 cagttaagta tggttcacat tctgcaccat ttaatataac tacttctgcc ttgctatctg
    36541 gtggaggaga tacttttaca tggttaggga atgtagctcc acccatacct actattcctg
    36601 cctcttttat gatacctact atatcttctt tgctcaaatc ctctaactga ccttttggtt
    36661 gaacactctc atgtaattct tccttaaagt cattttcgat aactacacat tgagctgaac
    36721 ctcctggtac ttcatgttgt tcaatagcaa taactttacc tgatactgaa gaatgaacgt
    36781 tacatgaaac aaaaccttgt tgttcaccta ttttttgccc aaactttact tcatcaccaa
    36841 cttcaacaat aggtttagct ggtgccccta tatgttgctg aagaggaatg taaactatct
    36901 ttggagcttg agctttttca agtgcctttg tgttagagta ctcttttcta tatggaggat
    36961 gtatccctcc cttaaaagtt aagagtttca ttgttttacc cccttatcta taaaaaaaca
    37021 ttttttctgc cttctataag tatactatat atattaaaaa atcttaacaa ttgttaaaaa
    37081 aatatccgta tttttataat ttattttata aatcaatacc tctatattaa gtcaaaccga
    37141 ggaaaaggat tgcttaatta aacaatcctt ttcatatagc tttctaaagt ccaaattttc
    37201 tgtattttat tttatagaat ttataaacat agagtactcc tgcatggatg ataaaagcag
    37261 acttttactt acactatatt tttcttcatt tgctgctttt gaagctgtat caattttttc
    37321 attcacattc tttacatact ctattaaatt atctttaaat aaacgcattt cagaatagtc
    37381 aatcttttca gcttcttttg atatattttg tgatattgcc tttctatttg tcaaaatagt
    37441 attatatttt tccatatcta cattattttc agcattcgac caaaaacttg attcttcttt
    37501 aaagtttgtt attaatttat ttaattcttt agatatactc tctatataag cgtagttact
    37561 tgtatactct aaagacaaca taggtgcatc taagtgtgat aaaaatgcat ctggaaacac
    37621 ctttcttaca ctgcttatct gctttctaac atcattttca tcaaaagagg aataggtttg
    37681 tatttttttt actccatcct tctccataac tgaaaaaggt atgtcattat taactaatga
    37741 tgtctgtatt tttttcaaat catcattatt atctattgca gcaacttgaa tagtgtacat
    37801 atcccagcct tctatagtag ctaacttgac ttcttctggc tctttagtgt tatttaccgc
    37861 tttttctgga ttagtttctt gtttatcatc tgaattggtt tgtgatttag attttttccc
    37921 tttttttatt gaatttaatt catcagatat atctttataa gttatatctg atgaattatt
    37981 tagaaatggt attttagccg atacatactc caatatctta ctatctttta tttttgtata
    38041 agaataatag ccaattagac acaagcaagc aatcgttata acatatttac gtaaatcaat
    38101 cttatgtcta ttattattaa atcctctgta tatcgttttt cttctattca ttagtttgtc
    38161 cctcccaaaa ttatattatt aataaagtat atttttaaga gtcacaattt atgaatataa
    38221 ataattattt cttctaattt tttaataaga aattgtactt actaaacaaa tcttcttgca
    38281 ccaagtaata ctttagacca atatgaacta gttaaattat ccactttaac cttattagga
    38341 ttagtctgag taccactaca atggataaat ttactattcc ctacatatat acctacatga
    38401 ttagttgttc catcaccatc agtgtcaaaa taaactaaat ctcctggtgc ataattcccc
    38461 atagaaattg cacttccaaa attagcctgt tctctagaaa ctctaggaat agaaacgcct
    38521 acagaatgtt taaaaacata ctgtgtaaat ccagaacagt caaatttatc aggtcctgaa
    38581 gctccccatt gatatggtat tccaataaga gtatatgcaa aatctacgat tgttgaacct
    38641 tttgctgtag atttatctga atttccagta aatgatggct ttgagttact attagattga
    38701 gaattttggt tagatgatga atttggtttt tcttttgtat cctcattagt ctcagaaata
    38761 taactggcac tcacccatcc aattgttcca ctcgataact ttaccttata ccaaccatta
    38821 cttttagctt ttaattcaac aacatctcca ccatttaatt ttcctattat agaatagctt
    38881 gtcccaggac cacttcttac atttaaactc acagcagatg ttaccttacc attcttgcct
    38941 tcaattgatt cttcgctaga tggtttttta tcagaattat tagtactatt tgaattacta
    39001 gaattgttcg ttccaacatc ttctgatgtt ttagaaatat attggctact agcccatcca
    39061 gtaactccat ttgatagttt tattttatac catccattgc tttgttctag taactttact
    39121 acatctccat tatttgcttt accaactaat gaatagttag tacctgcacc acttcttaca
    39181 ttcagtctag aactcacatt tactttacca tttccactaa tagttgtccc acttgaagtt
    39241 gaattatttt gacttgatgt accttcagac gaaccacttg ttgatatgta ttttgcactt
    39301 ccccatccaa caacagaact cgataccctt actttatacc atccattaga cttttctaaa
    39361 atctctacag tttttccctt gtataatttt gctaccttat cgcaatcaga acttggtcca
    39421 cttcttatat tcaaggcact tgctgttact gttccctttt caagagcact tgcatttatg
    39481 gagctaacag atactgctac tgccccaatt cctaaagctg ctatagcttt tttcactact
    39541 atcactcccc ccaacaaatt tttatagtca taaattcata acattattat ccatttttag
    39601 tccctacaat ttaatgataa cttattttct tattaattgt aaagaaatta taaacatatc
    39661 cttaaaatta gtgattttta ggaagtattt caatccaata attatctggg tccgctataa
    39721 aataaattcc catactttca ttttcaaaac atatgcaatc taaatcttta tgaagttttt
    39781 ttgcctcatc aaactcctca acttctaaag ccagatgaaa ttcattgtct cctaaattat
    39841 atggtctatc ccaatctcta agccaagtta attctaatgt atgactagta atgccatctc
    39901 ctaaataaac taatataaaa cttccatctt ctgcttcctt gcgctttacc tctttaagcc
    39961 ctaaagcttt tttataaaaa tctaaacttt tttctaagtt agtaacattg aaattgttat
    40021 gacaaaaatt aaacttcatt ttgaaatcac tctccaattt ttattttttt tatattatat
    40081 cattattata aatttattta aatcatatta agttaaaaat ttgaatgatg atgaccaatt
    40141 accaaatcat ggtaaattat ttaaaaaaat atgtcaaaaa aagaataagc tctttcaaat
    40201 gcataaaaaa tttataccca agttatactt agtcatgcaa attggcagaa cttattcttt
    40261 aaaactctaa cttttaagtt tttctttgat aatataattt attacttttt ttctaatatc
    40321 ttcagcatta taatcatctt ctacttctat atccaaaagt tttgctattg acctcatatc
    40381 ttctctagtc tttgacctat agcttgatat taattcatct tttattttct ctggttctaa
    40441 aagatattct tcatctcctc ttttgaaaac aacgtatttt tgagcatatg aacttctatt
    40501 gtagtatata tatcttgata tttttcttaa aatttgatta tatgaaagtc gcttgttaat
    40561 atcaagtttt aagtactctg aaaaagcaaa tagctctttt tttgatttaa ataatgcttc
    40621 tttctttata gtccttgata ttgaagttac actttcttga tatgcacact tttttaacat
    40681 ttttaaatat actctattaa gaccaatact ttgattgaat ttttttatat tattattaat
    40741 aatcttatcc aagttacctt caaagtattt ttttgctcct ttttgtgata cataactctc
    40801 aagttctttg tgggccatat caagtatcat tttttttaaa tcctctttat atcccctaga
    40861 ttccatgata ctccctccct tagctattta tagtatattc aaaaagctca agaaatgtga
    40921 gtcattacaa aattttttta ctttttatgt atatattaat ttaaaagggg agataatagt
    40981 aatgtaaaat caaacatact caatctcccc catttttaga cctctattac tcccccaata
    41041 gaggtctttt tttagtttat caatttctat tcaattccct ttagttcaaa tcctaattct
    41101 gctatcactt cttttaattt ttcaatagat acatcatctt tcatatctac agatgcacac
    41161 ttattttcta aatctacatc taaaacttca ataccatcta tatcttctgt taatgcagtt
    41221 ttaagatggt ttacacagtg accacaactc ataccttcta ctaatagttt cttcttcatt
    41281 taattacctc cttataccct ataggggtat tctatcatat agtttatact ttttaaatag
    41341 aaattaatca tttaatttga ttcattcgat tttatatcta aattagatat tccctcatat
    41401 acatcaaaat catttggagt aattttacct tttaatctac tattacttac aatataattg
    41461 tttctgacag ctaaacttat aaatgttgca ttatctttta tatatgtctg tacatctttg
    41521 tatgcttttt ttgtttcaat ttcagacgta gaacttgtaa gtgcagacat atatccattt
    41581 aacttatcat cagtatatcc actactcgct ataatactac ttgcatctgg tataattgat
    41641 aatttccatc caactaatgc taaatcataa tttttagaat taagagcatt ttccatatcc
    41701 ttatctgaca gctgatttac tgtactattt attcctatag cttttaagtt ttcatttatt
    41761 atgtttgcag tttttattct ctcactatta tctctgttta ctattatctt taagttcatt
    41821 tcagaaagac ttttctttac ttcctctttt tctctatttt ttttctcttc agcttctttt
    41881 ttgattctat cttcttctgc ttttttagct tttgcttctt cttctggcgt aagcttcttt
    41941 gagttctttt tagctgattt tttctgattt tttactttat tgtcattttg atttactgag
    42001 tttacatttg catattctac cttagcaaga cactctatcg ccttatcttt attaaactct
    42061 aaatccttca tttcactatt atagtatttt gattttgaat taagtggaaa attaactgga
    42121 gtagcatcat ccatataacc ttcactaata attctatctt tatctataga gtgtgcaatt
    42181 gcttttctaa aattaacatc tttgaaaaaa ggattattat aattaaataa tacacactca
    42241 taatctcttc cttgatattt tgttatatta aactcttttt cttgaaactt acttaaatca
    42301 tttaatgtaa cattggttat atcactatca agagccataa ccatagatgt ccttgcatct
    42361 tcattaggca ccatacctac ttttatgttc ttcatggttt ttggaacttc ttcatagtaa
    42421 tctttgttta cagataaaac cattccttct ctttctgtat atgattctat tttatatttc
    42481 ccatttccta tcatattatt attcttgtca tttacatctt ttgaatctaa atgattttga
    42541 gatacaattg gaaaaattaa actatcaatt gaaaaagcat atctagcttt aaattttatt
    42601 gtaaaatctt tatctgattt aatattcaca gaagctatat ttttagtaaa ttcattatat
    42661 ggactatctg cacttttttg aattaaacca atagtatagt ttacatcatt tgaagtaaca
    42721 ttttttccat catgccactt tgcatctttt agctttatat ctatactcat tccatcctga
    42781 gctatattat actccttaac taattgagga attacattgt agttctcatc tatagtaaat
    42841 aaactatcat aaactaaatt cattatataa ccaacagact tatcagtatt agttatagga
    42901 tttatagttt ttggagttac cattgttaag tttatatact tagcatctat agaactttga
    42961 ctttgtaaac ttgaatcact actactttgt tttgttttag tattactaca accagctacc
    43021 ataagagtta tagctaaaac gactgttaag acttttattc tcttcattag ttttttcctt
    43081 tcttgccata tatcttatta taatgttcgt aattattttt tactttttct acatattttg
    43141 aagtttgctt aaaaggtata tcatgtagat tttcgccatc tttactatat tcattatttt
    43201 ctaaccattt tttcacatta cctgaaccac cattatatgc agctatcact aaatctaatt
    43261 taccaaattc tttgtataac ttacttaaat accaacagcc aagtcttata ttagtctctg
    43321 ggtcgaaaat atccacattt tttaaattta gttcctctgc tccccaatct ctagttatgt
    43381 ctaatatttg cattaatcct tttgcttcct tttttgaaac agcagaacta ttaaacttac
    43441 tttcggcttt aataacactg taaactatat tttcatctaa attaaattct ttcgaatact
    43501 tttctacata ctctgaatat tttttaggat ataaaaattt atgtattact ttgctttcca
    43561 ttaatagtgc cccaaataag attataaaaa tagatagaat caatactttc ttactattca
    43621 cgccaattct ccttcatgta ctcaataaat ttatgtgctt tactttctaa ttcagttatt
    43681 gttcccgaat tatctattat ataatcacca tatttggatt tttcttcctg actcatctgt
    43741 gaatttatcc tactaagtgc ttcctgctca ctgcaattat ctctcttttg tattctacta
    43801 atttgtactt cttgtttaca agtaactact aatagcttgt caaccatatc taaaaaacca
    43861 ctttctacca atattgccgc atctaacact actatatttt cgcctttttt tctcaatttt
    43921 tctatttcac ttattatttt ttctcttatt cgtggatgag tcaagttatt taattcctta
    43981 agtttttctt catcactaaa aactattttc ccaagagctt ttctatttag agttccatca
    44041 tcatttttta tactttgccc aaaatggaca aatacttttt ccaataacaa cttatcctca
    44101 aatatcttcc tggatataat atcagcatcc actataggaa tgttaagatt tctaaatata
    44161 tttgataggc tgctttttcc acagcctatt cctcctgtaa gtcctaatat caacataaaa
    44221 tcacctactt tgtctcatac caagaactac ctacatttaa atctacatct aagtgaacat
    44281 ccatatttac agcattttcc atttcttctt ttactatttt cttaactata tctatttcat
    44341 catcaattgc ttcaactatc aactcgtcgt gaacttgtag tataagtttt gatttcaaat
    44401 tgttctcttc caatttttta tgaacattta tcatagcaat ttttattata tctgctgcac
    44461 ttccttgtat tggtgcattc attgcaaatc tctttcctct atttctttcc ataaaattgc
    44521 ttgactttat ttctggtata tatcttcttc tgtttaaaat tgttacagca tatccatcct
    44581 cactagcctt ttctactgtt gtgtccataa actcttttat tttatggtat ttagcaaaat
    44641 aactttcgat atattctttc gcctttggaa caggtatatt taaatcatca gctagaccaa
    44701 agtcactttt accatatata ataccaaagt ttacagcttt agctgcacct ctcaattcac
    44761 tagtaacatc ttccatagga acattaaaaa cttgtgaagc tgttttggta tgaatatctt
    44821 catgatgttt aaatgcttct atcatatttt catcctgact catatgtgca agaactctta
    44881 gctctacttg tgagtaatcg gcatcaacca acttacagtt atcatctgca ataaacactt
    44941 ttcttatttt tctacccatt tccattttta taggtatatt ttgaagattt ggttctgtag
    45001 atgaaattct tcctgtagtc gttatagttt gattaaatga tgaatgtatt ctattacttt
    45061 ctggatttat tatatttaaa agtccttcaa cgtaagtaga atttaacttt actatttgtc
    45121 tatactcagt tatcttatct attattggat gcttatctct taatttttct aaaacatctg
    45181 cattagttga atatccagtt ttagtttttt taattattgg aagctctaat ttttcaaata
    45241 atataacacc caattgtttt ggtgaattta tattgaatgg ctctcctgat aattcataaa
    45301 tttccttttc taatgtagct attatttctt taaactctac actcaattca tccaatatac
    45361 ttttgtctac ttttatacct tcatactcca tatgacctaa gacttctact aaaggcatct
    45421 ctacatgata aaaaagacca tccatactca tatccattag acttctttcc atcataagaa
    45481 ctgtttcttt aactgtgcat attatttgac ccatatactc tgctaaatcc tcaaattcta
    45541 aatcctcata gtttttagat ttaacaccct ttcctaataa ttcttctttt gatttaacct
    45601 tttttgataa atacttcata gctattgcac tacaatcata tgaggtagat gaagttgaat
    45661 ctattaagta ctcagctatt gttatatcaa aatacatatt ttctaagttt atattatatg
    45721 gttttaaaga tatataatca tcttttaaat tatatccata ctttttaatt tcattatttg
    45781 aaaatatact atctaacttt gacacttcat ttgaatctac ataatatatt ttttctccat
    45841 ctacacttat aaaaatatat attatatttt tttcaagtat attaccttgt cctctaacag
    45901 tttttaaaat aacttgtcct tttttgttta cttcatctat aaatttttct atatctttta
    45961 attttaatat ttctattttt tcttctacgt gttctgtgtc atctgaatcg accaaatcaa
    46021 ctaatcttga taagagactt gtaaatccaa agtatctaaa tttatctaaa agttcatctt
    46081 tatcataatc gccaaaaatc atactatcta aatccacttc tattggcaca tctcttataa
    46141 ttgttgcaag tcttttactt tgaatagcta tttctttgtt ttcctctatt ttctttttta
    46201 cactaccttt aagtttatct gtattctcta ttaaattctc tatactagaa aactctttta
    46261 taagctttat acctgttttt tcaccgattc caggtactcc aggtatatta tctgacttat
    46321 ctcccataag accttttaaa tcgataaatt gtgttggagt catctcgtat ttttcaatta
    46381 cagaattaaa gttgtattct tcaacttctc caactccttt ttttgttata agagtagttg
    46441 tattgtctga agcaagttga atagcatctt tgtctccagt tactatgtac accttatacc
    46501 cattattttc tgctttttta gaaacagttc ctataatgtc gtctgcctca tagccatcta
    46561 tttccaatct atttattttg aatttatcta aaaggtcttt taatggctgt aattgttctg
    46621 ccagttcatc tggcattccc tttcttccag ccttatactc tttatattcc aaatgtctaa
    46681 atgtaggtgc ttttctgtca aatgctacac ttatatgtgt tggtttatat atatctatca
    46741 ttttaaatag cattgttgta aatccatata ttgcattggt ctttaaccct tctttattat
    46801 tcatttctgg cagagcataa aaggctctgt ttattattga atttccatct attataatta
    46861 attttttttc caaattatat cactcctaaa aattatctat ttttgtaaca tatatattat
    46921 ataccaatat agtgttattt aatattccca taataataat agtatattaa aattaaatat
    46981 gttgcaaaca atatattttt ttgctataat aatatacatg acatgcccga gtggtggaat
    47041 tggtagacgc agtggactca aaatccaccg gactaatact ccgtgccggt tcgagtccgg
    47101 cctcgggcac cattaaaaac tcctaaagct atttattcaa tagattagga gtttttttat
    47161 ttttgattta aattttaagt tattttttga aattactgtg tctttttaaa atttatataa
    47221 ttatataaat ttatcttcta gcattaattt aaaaactaat ttacttttat ataattttct
    47281 attttatatc ataacttatt acaacctttt atctaattta ttgtatacaa caacactaca
    47341 aataaattga aataaaataa gcaatccaag aacaactaaa acccacttgc ttattaccat
    47401 tacagaaaat acactcaata tagaaacaat aaatataaga attacaagaa catagcacat
    47461 gatttgatta gtaatacgtc cagctttatc acgtaacata attcttcttt catcattcat
    47521 ctctatcctt tgatttttta tcttttcttc ataatctact gctctttcag gtttactcca
    47581 atacatatat tgaagaatca tacatacacc tggacctaaa gcagctccag ataatcccca
    47641 aataaaagcc tcaaatgaaa actcagtaaa tgtagccaat aaaacaagta aaattccaca
    47701 aataaaatat ataattccaa catataacat attctttttc atttttactc ctcctcataa
    47761 ataaatattt cttcaatact catatcaaag tatctagcta tcttaaaggc taagatgata
    47821 gaaggattat acctaccatt ttctaatgaa cctatagttt gtcttgatac ttgcaaaata
    47881 gaagcaagtt cttcttgttt tatgcctctt tcttttctta tttcttctaa tctatttttc
    47941 acatctatcc ccctttaata tctcaactac ggaaagtcta ctttccataa ttatacaact
    48001 atattccatt tatgtaaagc ttactttcca tatttacaaa aaaataacag actattttca
    48061 gcctgtaatt ttagaatttc atattgttta ttaattttaa tgttatttat taattccata
    48121 ttatttatta attttaactt ggtttctgaa ttggaatcca aacttctgta gtataatcag
    48181 gagcattgat atcgccattg ggataaagtt caatatctgg taaattagca tacacatacc
    48241 cagaacttgg taaccactca gtaataattc ttttttgcaa ttcctgtatt gccagagcat
    48301 ttggcattgg agaaacacat tcaaatacta cccatgtgcc tgatggtatt aagtattcat
    48361 gagtatcttc taatacaggc ttatctgttg gagcagcaat ataataatct aaacccttac
    48421 tatttgtaca cacactaact ccaagcaatc cacaaggttc gttatttatt aactctgaaa
    48481 gtttatcaat tgttccattt tttatgcatt tagcccataa cttaggaact ttctcaaaac
    48541 actcttctac aatcatgttc atactttctt ttataccaac aatacgaaac cctttctttt
    48601 ctacaatacg atattgcatt tctatatttc cttttacata aatgcttaat gtcatcttag
    48661 gatatgcttt taaaacaaca ccctttgctc tagctgcaga tggagaaatg ttatgtatat
    48721 tttgaaaagc tctattaaaa gatgttggtg aatcgtaacc atacttctca gatagttcta
    48781 aaattttgat gttactgttc tgaagttcaa atgcagcctt agtcatacgt cttcgtctaa
    48841 tatattcaga taatggtact tctgcaatat atgaaaacat tctttgaaaa tgaaaggttg
    48901 aacaacatgc aatttttgct gcttctgcat aatccactcc actttcgaga ttttcttcaa
    48961 tataagtaat agcttgattt aatttattta tccattccat ttgatattac ctccaagcaa
    49021 attatacagt ttaataaaat aatatacctc tctattcatg cacaaaattg taagcattta
    49081 aatttataag catttttcaa tcaatacaat aaatataaaa attgtattat acaataaaaa
    49141 agttcaataa cattatattg taaaatgtta ttgaactttt tataaaaatt aatttaagtg
    49201 tagttaaatt aagtattaca ctatcttata tattacaata tcctactatc ttatagtctt
    49261 ctggcacttt ataatctttg tctggagtac ctaaattcca ttttaaatca aacatagttg
    49321 cacttattat tgttgcaaaa taaagcataa ctataccaat gtctattttt tcttcatata
    49381 aattagtatg accatctttt ctaacagcta aaactacagt gcctccatca actataaatc
    49441 tccatggttg tctatttaat gtagatggag ctaatcttgc atagtgaaat gcatcaagaa
    49501 gtgctctttc ttctaagatt tctatacttg caacatttcc ccattcgttc atataaacaa
    49561 catcttctac acctagtcta gtagacccct tatctgttac aacttgcata tcagattttg
    49621 aataattttg cccagttttt gtagcattta atactttagc attacttaca ttttctccat
    49681 atccaatagc tataattcct gttacctctt tatctgaatt aattccaagt ttttctttta
    49741 ccttttttcc atcttcaaaa gtaacccaac aagaaccaat tccaagttct gttactttaa
    49801 agattatact ttctcctatg taccctgaat tttcaatgta attatctttt atgtcagata
    49861 atattataat ataacttgga gaatctatca tatttccttt gtaccctgct actttatcta
    49921 aattattaaa aacttcactt ctattcataa ttcttacatc aacatctatt tctggaacta
    49981 gtttcttaca agattctgag tatttcttaa tttcactaag atattcttct ttaatatctg
    50041 tgttcttata ttctctaaca gactttctat ttaatatgat tgttttataa tccataattt
    50101 tatcctcccc aaactagtat tgtcttaatt atatacccat taatatatta ttttactcat
    50161 ttatttaata aataaagatt acaattttgt aattattatt tattaataat attccacttt
    50221 tacattaata atgaccagtt gtgcaaaatc cattgccaac caccaaataa aaacatatta
    50281 taaaggtaat ataaaataac aaacaaatct taacaaaaaa ttaaactttt tttacacgaa
    50341 agaaggcttc ccgttgcctt ctttattttt ataaaaaaat aagctgtttc taaaatgatt
    50401 tcacattttt gaaacagcca agttattcta taatgacaac tataatttga ggatgtgagg
    50461 gagagttgtc attataaatt tagggtaatt atattatgtt ttaagctagt tctttctata
    50521 tttatataat acattttata tatgaatttt atatgagatt ggtataaatg atatatgaaa
    50581 ttacatatgt ttatattata ttaaatttta ttattacatt ttccacaaca atactcatat
    50641 gtctccacat tatttggaat atttacaaat acaaattctt tacagttctc acaatacttt
    50701 aatatagata tagttccaca agtgtaacat tcaaatatta cacaatcctc attttcctct
    50761 actatttcaa actttttact atgttcacca cactctgggc acttgtgatt atttacattc
    50821 ataaatactc ctccttatta gtatagttac atgtcaaaaa cttaattaat gtattctata
    50881 ttacttatta tatattctat aataaatcat attctaatat aataaaacaa attaaataaa
    50941 aaaaaaccat aaattttaaa tttatggatt aattcttgta atgataactc tatttgagga
    51001 tgtgagggag ggttatcatt acaatagggt attatattat ggtttttaag ctagtcttt
//
LOCUS       DBGJFF010000010        49124 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.010, whole genome shotgun sequence.
ACCESSION   DBGJFF010000010 DBGJFF010000000
VERSION     DBGJFF010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 49124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49124
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.010"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(100..1380)
                     /locus_tag="ACOIAF_000683"
     CDS             complement(100..1380)
                     /locus_tag="ACOIAF_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HGZ2667709.1"
                     /translation="MNILGLIVEYNPFHNGHIYHLSKSIEKTNATHTVAIMSGNFLQR
                     GEPALFDKYTRAKIAVENGVDLVIELPTLFACQSAEIFSHGAITILNSLNCINSICFG
                     SEEGNTEILYNIAEILANEPNKFKLLLKKYLDDGLLFPTARANALFDYIHQDKKNNFI
                     QNYSKEQLSKTLNSSNNILGIEYIKNLIHLNSSIKPFTISRVQSEYNSEEIESDICSA
                     TAIRTSLKENTDLFHLKNVIPEKTYNILNEKIDNGFLPMFDDFFFDILKTIILRDMDT
                     LKEYFEVNEGIENRIYKDIFTSEFLHELQLAIKSKRYTLTKIKRTLNNILLGIKKSDM
                     QLIKNIDTIPYIRILAFNDKGREILKEIKNKSEIKIINKFSNISFSLDDTIFKTLISY
                     DIKATNIYNSIYYKNNKDLVKGPMDFYTHPIYVK"
     gene            1843..3039
                     /locus_tag="ACOIAF_000684"
     CDS             1843..3039
                     /locus_tag="ACOIAF_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HGZ2667710.1"
                     /translation="MKILVLNCGSSSLKYQLIDMNNEEVLCIGLVERIGIEGSILKHE
                     KAGRDDKYVVEQPMKDHKDAIALVLEAVAHPEFGAVKEMKEIDAVGHRVVHAGEKFAT
                     SVVITPEVEEALKECIDLAPLHNPANIMGIDACKAILPDVPMVGVFDTAFHQTMPKSS
                     YLYGLPHELYTKYGVRRYGFHGTSHNYVSQRAAEILGKDIKDLKIVTCHLGNGASIAA
                     VDGGKCVDTSMGFTPLEGLIMGTRCGDIDPAILPFLMRKEGLDADGLDKLMNKESGVY
                     GMTGISSDFRDIEDAAKNGDERAQATLEAYVKKVQKYIGAYAAEMNGLDVVVFTAGVG
                     ENGKAIRADIASNMEFLGMKLDKEANDVRGKETVISTADSKVKMLLIPTNEELMIARD
                     TLRLVK"
     gene            3132..3656
                     /locus_tag="ACOIAF_000685"
     CDS             3132..3656
                     /locus_tag="ACOIAF_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HGZ2667711.1"
                     /translation="MKVSIEKINRKETDKIDLNFCEKIDTISYCDEIYKLVSPVNLKG
                     KVSKTNKGLYLDIDVNFTIVDNCSRCLKEVEIPLEYSIQGFLVKEEDYDEDEFEEFDP
                     FIFDGEEIDLIDIIEQTLDFNVPHKVLCSENCKGLCQVCGANLNEEECSCSEITNDEE
                     YIDPRFAKLKDLFN"
     gene            3681..3857
                     /gene="rpmF"
                     /locus_tag="ACOIAF_000686"
     CDS             3681..3857
                     /gene="rpmF"
                     /locus_tag="ACOIAF_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HGZ2667712.1"
                     /translation="MAVPKRKTSKSNTKMRRAANSKMEATGFVSCPQCHEPKLPHRVC
                     PDCGYYKGKEVVSK"
     gene            4064..4621
                     /gene="fapR"
                     /locus_tag="ACOIAF_000687"
     CDS             4064..4621
                     /gene="fapR"
                     /locus_tag="ACOIAF_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045717 - negative regulation of fatty acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HGZ2667713.1"
                     /translation="MKKKSKAQRQKELIDMLKTDPFYTDEELSSLFDVSIQTIRLDRM
                     SLNIPELRERVKSIAETQSSKVKTLGVKEITGEIIDLSVGRLGISMLEVTQDMIYSKT
                     NTLKDTYIFSLADSLAMAIIDAPKVIMRVANVKSFKLIEQQDRLIAKAEVYRNIDKKH
                     YVKVVINNKAQEQIFRGKFIFEELD"
     gene            4632..5654
                     /gene="plsX"
                     /locus_tag="ACOIAF_000688"
     CDS             4632..5654
                     /gene="plsX"
                     /locus_tag="ACOIAF_000688"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HGZ2667714.1"
                     /translation="MKIVIDGMGGDNAPKSNVEGAVNAIKEYQVDLIITGDKDLLEKE
                     FSNYEFDRNKLEIVHTTEIIENEDKPVKAIRSKKDSSMVVALNLVKEGKADAIISAGN
                     TGALLAGGLFVVGRIKGIDRPCLCSAIPNVKRGMTLIADCGANADCKPKNLVEFAAMS
                     NIYSRKVLGLENPKVALANVGLEEGKGNDLVKRSYEEIKKLDLNFIGNVEAREVINAY
                     TDIIICDGFTGNILLKSAEGVALSVMSLIKETFMASTKSKIGALLIKDDLRKLKSFID
                     YSEYGGAPLLGLNGGVIKAHGSSDAKAIKNAINQGIKFSKGKVVEDINQFISKYNEEN
                     KNNEDE"
     gene            5680..6666
                     /locus_tag="ACOIAF_000689"
     CDS             5680..6666
                     /locus_tag="ACOIAF_000689"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HGZ2667715.1"
                     /translation="MNTKAGILGVGSYLPEQSYDNFHFEKIMDTSDEWISTRTGIKER
                     RFAKESEATSDLASKAALKAIECAKLNVEDIELIILATITPDMSLPSTACIVQDAIGA
                     VNATAFDISAACSGFVYGVTIAKQFVETGCYKNVLVIGAETCSKFLNYDDRTTAVLFG
                     DGAGAAVIGPVNEGGILSTHMGSDGKGKDCLKVPAGGSRLKASKETVEANLHTIEMAG
                     SDVFKFAVRKMAETSLRALEKANLNTTDIDYLVPHQANIRIIQASSKRLELDMKKVYV
                     NIDKYGNMSAASIPVALDEAYREGKIKKGDNVVLVGFGGGLTWGASVVKWTL"
     gene            6775..7704
                     /gene="fabK"
                     /locus_tag="ACOIAF_000690"
     CDS             6775..7704
                     /gene="fabK"
                     /locus_tag="ACOIAF_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HGZ2667716.1"
                     /translation="MNKICKILNIKYPVIQGGMAWVATASLASAVSNAGGLGIIAAGN
                     APKEAIKKEIVECKKLTDKPFGVNVMLMSPFVDDIIDLIIEEKVQVITTGAGNPAKYM
                     DRLKEAGTKVIPVVPTIALAQRMEKLGATAVIAEGTEGGGHIGELTTMVLVPQVADAV
                     NIPVIAAGGIVDGRGIAASFALGASAVQVGTRFICSEECSVHSNYKNLVLKAKDRDAI
                     VTGRSTGHPVRTLKNKLSKEFLKMEQNGATPEELDKKGTGALRFATVDGDIEKGSFMA
                     GQSAAMVKEITPCKEIIEAMVNQAREIMPAIEL"
     gene            7720..8670
                     /gene="fabD"
                     /locus_tag="ACOIAF_000691"
     CDS             7720..8670
                     /gene="fabD"
                     /locus_tag="ACOIAF_000691"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HGZ2667717.1"
                     /translation="MGKVALVFPGQGAQYVGMAKDLYENNAVAKSVIDEASDALNMDL
                     RNLMFNGNEEELSKTENTQPAMVTHSVAVLKAVQAQVDLKYSACLGLSLGEYSALVAA
                     DAIDFKDAVCLVKKRGKFMQDTVPTGVGAMAAILGLDRSILENVVKDIQGGIVEVANY
                     NSPGQIVISGENEKIEEAMVKCKEAGAKRAVKLNVSGPFHSSMLREAGVKLGSELEKV
                     NITKPKIDVVANVNADYYKSEGKELLIKQVSSSVLWEDSIERLLNDGYDTFIEMGPGK
                     TLKAFIKKIASNKKANVNIYNIDGIDSLNEFVQNYRNGEI"
     gene            8670..9419
                     /gene="fabG"
                     /locus_tag="ACOIAF_000692"
     CDS             8670..9419
                     /gene="fabG"
                     /locus_tag="ACOIAF_000692"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HGZ2667718.1"
                     /translation="MINLTGQVAVVTGGSRGIGKEIAKKLASFGADVVINYTSKEDEA
                     LKTKNEIESMGVKCTSIKCDVSKFDEVNQMIDSVVSEFGKIDILVNNAGITKDGLLMR
                     MKEEDFDRVIDINLKGVFNCTKAVTKPMMKKKYGRIINMTSVVGIMGNAGQTNYCASK
                     AGVIGFTKASARELASRNININAVAPGFIETDMTKVLSDDVKESTLANIPKKSYGKPE
                     DVANAVAFLVSDMSSYITGQVINVDGGMVMQ"
     gene            9473..9697
                     /gene="acpP"
                     /locus_tag="ACOIAF_000693"
     CDS             9473..9697
                     /gene="acpP"
                     /locus_tag="ACOIAF_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HGZ2667719.1"
                     /translation="MFNKVVEIIIEQLGVEDKEITMETSLMKDLEADSLDAVEIIMAL
                     EDEFGIEIPDTEAENFKSIGDIVKYIEANK"
     gene            9726..10964
                     /gene="fabF"
                     /locus_tag="ACOIAF_000694"
     CDS             9726..10964
                     /gene="fabF"
                     /locus_tag="ACOIAF_000694"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HGZ2667720.1"
                     /translation="MNKRVVITGLGCVTPLGTGKEEFWSNIKSGVSGIDKITNFDAST
                     YQTQIAGEVKNFHPEEYISKKELKRLDKFAQFAIVSAKLAVEDANLDLDKVDRERFGV
                     IIGSGIGGVEAIETQHKILLEKGNKRVSSLFVPMMIGNMAAGQVSIFLGAKGPNTNVC
                     TACASGTHSIGDAFKVIQRGDADIMVAGGSEAAVTGLAFAGFCNMKAMSTRNDDPKTA
                     SRPFDKDRDGFVMGEGAGIVILEDLEHALARGAKIYAEVVGYGLTADAYHMTTPAENG
                     EGAARSMNMALKDGNVPLEEVDYINAHGTSTYYNDLYETMAIKTVFGEKAYDLCVSST
                     KSMTGHLLGASGAIEAVVCAMSIEDSFVPPTINIQEVGEDLDLDYVPNQGKEKNIRYA
                     LSNSLGFGGHNATIVLKKYV"
     gene            11190..12908
                     /locus_tag="ACOIAF_000695"
     CDS             11190..12908
                     /locus_tag="ACOIAF_000695"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HGZ2667721.1"
                     /translation="MNFRLPKKRLPKKIIFICIILISIILFIKYDQNGLKEREIINKG
                     KYMSESLVADKEYDEAKKVVKTSFESIPEKKYNKVYNEVWDMLKTISYVPDGTKIVID
                     SLEKMRESDVKLSDECRFNIEKRLASVYIMDNNYSKAVDLTVKSIKLAEKLGNNYEKA
                     RLDVDLASIFLKIGGYETGEKLIKDSFKIDIDEGEKNGMVRLYAIINLAEIYVEEGEY
                     DKAIEISSRIKDYKKFVNTDNYNDFDIMASIMQSNAYVGKNNIQKAKTFLEKADYLIK
                     SDRTVYILDKDMYYYMAMGNLEYKSENYNSAIDNYKKSLEISTKRKLTQEKIKNLNKI
                     LNIYEKQGNIESLNEYQKMLINEYKENKSIRDSETSFYIIDKVSNESKLFEKTKKEIK
                     YYKILFTVILIAILGSTFLYNRLKYFKAQNLHDGLTNVYNRKSFDIMYEKYREKDDNF
                     ALVMIDIDNFKLINDNYGHKFGDTVIKGITNTICVMLEQDDKVFRYGGEEFSVLIRNK
                     SGEEVADIIDSIRVAIANKKWNDDVTVTISAGVAHISDSKDVLEEADKNLYKAKQLGR
                     NKVVYK"
     gene            12939..14939
                     /locus_tag="ACOIAF_000696"
     CDS             12939..14939
                     /locus_tag="ACOIAF_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HGZ2667722.1"
                     /translation="MKQSIAIVTDKITKLSSFLEENIRLVLGNYIDINHYYLEVLREN
                     DKIQGDFVLVMNDDRLNSMRKHLVQDSKIIVIRRTLKENEIYGLFSIPNGTEVLVVND
                     TKETTLETISLFYKIGVKNLKFTPYIEGKIYKNIDIAITPGVKGKVPSYINKVIDLGN
                     RYIDISTFIEIINGLRIDVKEIRKNLMAYSESLISLDNGVKDNYRQLFLKIEEQDVIL
                     NLSKDGIIFTSADGIINTFNKEALKILGINENPQGKNIEDIISEDLNILLEDKEIIDE
                     VVFTNKKYINVNKKNIFSMGNKAGIYYNLQEITYIKKLEQNLTNKLREQGQIARYTFK
                     DIKTKNKNMVKCVELAKKISKSDLSVLIIGESGTGKELLSQSIHNASNRSNQPFIAVN
                     CAAVPDSLLESQLFGYEKGSFTGALREGKKGLFELANNGTIFLDEIGDMPPLLQTKLL
                     RVLQEKQVMPIGSHNVINIDVRVIAATNKNLLKMIREGKFREDLYYRLNVLPIDVPSL
                     RNRKEDIITLMNFFLKNNIRLSPEVITILEKYNWPGNIRELQNVASYVSLMCDSMVFR
                     DDLPPYIRVECANNYEDGFGYTRKEFEEILRILYERRESSSGIGRGFIIEKLIQEGIE
                     ISESKVRKVLSYLSECGYISSKSGRGGSQITSEGIKLYNNIN"
     gene            15290..15535
                     /locus_tag="ACOIAF_000697"
     CDS             15290..15535
                     /locus_tag="ACOIAF_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HGZ2667723.1"
                     /translation="MKYKTTGTCATEIEFEVKENKVTNVNFIGGCDGNLKGLKVLVEG
                     MNIEDVIQKLKGIECKTKPTSCPDQLSLALENYMNIK"
     gene            15570..16295
                     /locus_tag="ACOIAF_000698"
     CDS             15570..16295
                     /locus_tag="ACOIAF_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HGZ2667724.1"
                     /translation="MKPIIGILGNLIIMENGMFPGLERSYVNNDYINAVLKGGGSPVI
                     IPVNTDKEVIKKQIEMVDGVLISGGWDINPQLYGEETREETTFIYPEVDEFDLIAISI
                     ALELKKPILGVCRGLQILNVSLGGTLYQDNNLIEGSYIKHTQSSKRHVATHKVDVKEG
                     SILEGILGKQLLTNSYHHQSVNQLGKGLKAIAYSKDGIIEAIEKEDENFVVGVQWHPE
                     MMVDYCDKMEKLFKYFINICSNL"
     gene            16317..17630
                     /locus_tag="ACOIAF_000699"
     CDS             16317..17630
                     /locus_tag="ACOIAF_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HGZ2667725.1"
                     /translation="MNEKNKMGLISIILLGINAVVGAGVFLLPGDAMKSFGVASIFVY
                     IFDMLLVLSMAFCFAEVAGKFNKNGAAYVYTKEAFGDFCGFEVGLMKWVIGCISWGAL
                     IVGFPTSLSAVWAPAGEPHIQKIIIVAMIVGLTIINLLGVSLSKIVQNVITVGKLIPL
                     ILFIGIGIFFIKGVNFTSSTMVPPGAGATEFGAAALLMFYSFTGFESIAVAAEDMENP
                     QKNIPIAIISVIVIASIIYILNQVVCVGILGDSLSSTSTPVADAARICFGNMGAGLVT
                     FGTLVSVGGICMCGAFVNPRSCVALADDKMLPRIFARKDKKGTPYVAIIATMLITIPI
                     ALSGSFAELAAISAVARFIQYIPTSLSVLVFRKKRPELVGTFKTPFGAVIPLIAVCVG
                     TWLLFQASMHQLIMGLGALAIGVPLYFIMKSYNRKVYGENLKKIV"
     gene            17836..18375
                     /locus_tag="ACOIAF_000700"
     CDS             17836..18375
                     /locus_tag="ACOIAF_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HGZ2667726.1"
                     /translation="MPTITLKNGVDVLIREGVREDAQSIIDFYNEVGGETHFLSFGKD
                     EYKISLEEQENAIESAKASDNSVKLIAFIDGEIVGIATIDSNQKAKGKHVGVLGIVVK
                     EKYWGIGLGKRLMLDLIEWCKSNGITKKITLVTNEENYNAIGLYKKVGFEVESILKKE
                     CYYNGVYTDLIGMSLLLGI"
     gene            18464..20449
                     /locus_tag="ACOIAF_000701"
     CDS             18464..20449
                     /locus_tag="ACOIAF_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HGZ2667727.1"
                     /translation="MKNLCKISDDYLNSKLKYLKLLSKQYPSISKASTEIINLEAILN
                     LPKGTEHFITDVHGEYEPFVHVLKNGSGVIKRKIEELFSNTIRDSEKKMLATLVYYPE
                     QKLDLIIKQEENIDDFYRINIYRLIELCKYASSKYTRSKVRKLLPENFKYIIEELLHE
                     HVKSEHKEEYYKSIVETIVDIGIAKEFIIAISTVIQKLVVDRLHVIGDIYDRGPRPDI
                     IVDKLIEHHCVDIQWGNHDILWMGAASGEKTCIANALRISARYANLDIVEDIYGINLL
                     PLATFAIEMYKDDPCKEFIPKVNDQSVTTTEKSLMAKMHKAISIIQFKLEGEVIRRRP
                     EFEMEHRLLLNMINYDEGTINLKGKTYKLKDTYLPTIDKKDPYKLTMEERNVIDKLVS
                     SFRGSEKLQKHVSFLFSKGSIYLKANSNLLIHGCVPLNEDGSFMSMNIMGKEYKGKAL
                     MDKMESLAREGFFFKDKAEEKLYGMDIMWYLWTGKCSSLFGKDDMTTFERYFIAEKET
                     HKENKNPYFKLRENEMACKRLFEEFDLELDESHIINGHVPVESKNGESPIKANGKILV
                     IDGGFSRAYQKTTGIAGYTLIYNSRTLQLVSHEPFNSAEEAIANESDILSTTVVVEHK
                     AKRKMVRDTDEGIKIQEEIEDLKLLLMAYKKGLIKEM"
     gene            20673..21641
                     /gene="spoIIIAA"
                     /locus_tag="ACOIAF_000702"
     CDS             20673..21641
                     /gene="spoIIIAA"
                     /locus_tag="ACOIAF_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AA"
                     /protein_id="HGZ2667728.1"
                     /translation="MNKLSDEIINSLSTTIREKVEKVSNNNLNIEEIRLRSQKPLILN
                     ANSKDYFYNQKTMTLDLNQQNSYVVTREDVEQTFQIICKYSIHSFMDDIKKGFITLRG
                     GHRVGLVGKAIVEDGQVKNIKHISSLNIRVSREIIGCSDKILSHIIKGKNQINNTLII
                     SPPQCGKTTLIRDIVRNLSNGNEDYGFKGLKVALVDERNEIAGAYLGVPQMDVGIRTD
                     IIETCPKDLGITMLLRSMSPNVIVTDEIGSEKEIKALYTALNGGIGLITTVHGDSIED
                     IQNRKELNRLLDKELFKKVIILSAKRGAGTIEKIYDLEEKRWYFAN"
     gene            21631..22152
                     /locus_tag="ACOIAF_000703"
     CDS             21631..22152
                     /locus_tag="ACOIAF_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AB"
                     /protein_id="HGZ2667729.1"
                     /translation="MQIKIIIIAFLIGSSYLIGEQIYKTYTRRHKQLNDLIRVLEILR
                     MDLSFGLYTLEEIFNRIGGNKEFCFWKFFYQISEGLHNEQSKTLEIIISENIDVLSKE
                     TYLGNKEIEELKNLILTLGKSDIESQQRMIDLSIENLKKQTYETKEDINKKGVLYKKL
                     VTFIGIGICIILI"
     gene            22169..22366
                     /gene="spoIIIAC"
                     /locus_tag="ACOIAF_000704"
     CDS             22169..22366
                     /gene="spoIIIAC"
                     /locus_tag="ACOIAF_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AC"
                     /protein_id="HGZ2667730.1"
                     /translation="MEISLILKVAGVGILISVLNMILEKTDRKDWAGLTTLAGVIIVL
                     GMVITEISDLFNTVRTMFQLY"
     gene            22391..22768
                     /gene="spoIIIAD"
                     /locus_tag="ACOIAF_000705"
     CDS             22391..22768
                     /gene="spoIIIAD"
                     /locus_tag="ACOIAF_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AD"
                     /protein_id="HGZ2667731.1"
                     /translation="MQLIGIAIISTTLCLVIKKDRPEIANFIAIITGVIILLSVMFKL
                     NFIVDSIQDLANKANIPTMYISLIIKLIGIAYLMEFAIQLCKDCGEGNIASKLEFGGK
                     IIVMSMSFPILLSIVEMVVNIIP"
     gene            22853..24058
                     /gene="spoIIIAE"
                     /locus_tag="ACOIAF_000706"
     CDS             22853..24058
                     /gene="spoIIIAE"
                     /locus_tag="ACOIAF_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AE"
                     /protein_id="HGZ2667732.1"
                     /translation="MKKRFLPMIIGFLFTFFFVNIFAIMIFANEVPSSEDKEYGETKN
                     SIDKYIDGQLDKLDINEIQDYINKEIVINDVNLKSFVKDLISGEKNILDLFNKDGLKI
                     LMFDEFKASLKVVAVILVLALLSSILKSLENSFSSGAVSQIATYIIFITMVSLTLIGF
                     KDVLQICYDAIDHTVGLMQVIMPILITFLLLIGFPITSTTLNPIFIGGVTFINVFFKN
                     FLFVSITVAFGILIINNLSKNIRLKRFFSFVKQINYVSIGAMFTVYLGLVSIQGLYVT
                     SFDKFSVKTAKFAIGNFIPVVGGFVSDSVDILLSSSQLIKNIFGGIGLILLVGICLLP
                     VIKILSVIVVYKLAAIIVEPVGEDGISNFLNEVANLMIIMLASVIAITVMFFVTVAIL
                     TSISVVSQG"
     gene            24118..24735
                     /locus_tag="ACOIAF_000707"
     CDS             24118..24735
                     /locus_tag="ACOIAF_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AF"
                     /protein_id="HGZ2667733.1"
                     /translation="MLEGIKAWIVSVLIGAFIVNIVDMILPSSKIKPYVNLVLNFMFV
                     FIVITPVVGFFSKDMSLEDRILKSMGNYNKQYVDSTNALAKETGNNSLSKGYEDGLKE
                     VLKLKLDEYGYDLEDIELNGANINNIKIKEKNNSAKSNNSSNEENKNNSTKREEENIN
                     SNDKENSKQVFKKGTEYGLNLNEEKLKNDLIKVLDVSIEDIQIDK"
     gene            24747..25301
                     /locus_tag="ACOIAF_000708"
     CDS             24747..25301
                     /locus_tag="ACOIAF_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AG"
                     /protein_id="HGZ2667734.1"
                     /translation="MLKNLNDKDKRKVYSLITIAGICVVSLVLLSCFPSSKSDKQVGK
                     TETNKNAEKQVTKEQEKDDLESKLTAILSKIDGAGDVDVMVTFESSEEIQPAFNSNNT
                     TETTEEKDAQGGERTVTTSSENKTMITSNSSDPVVIKTTEPKIKGVIVVASGASDPSV
                     KETLYSAVQTSLQVAGHQVEIYSK"
     gene            25328..26017
                     /locus_tag="ACOIAF_000709"
     CDS             25328..26017
                     /locus_tag="ACOIAF_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAH-like family protein"
                     /protein_id="HGZ2667735.1"
                     /translation="MKFNYKGRGFVIITLTAMLVVVGTVNYQLSKKSLLETSKEFKAY
                     EEAQLQKNTDDSDSSKTEDSSNVDKQGGKESADIDIVDSKASKVKEKATETSKEIKAQ
                     LSSEKNMKKASYILDMKMNREKQRNELVQDLNEMINNPSTTEESRKEASNMKLNIVKI
                     QEKELQIENLLSTKGYEEALVYISDNKVNVVVNEAKLEKKDAAKIFDLVAEQANVKYE
                     NIKLTNNNSNK"
     gene            26127..26489
                     /locus_tag="ACOIAF_000710"
     CDS             26127..26489
                     /locus_tag="ACOIAF_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HGZ2667736.1"
                     /translation="MEDNKFGQVKISNDVIATIAGLAALEVEGIETTATLTDKLLKNN
                     GVKIQIEEEDVNLDVMVTIKYGMSIPDTAFKVQENVKNTVETMTGLKVSQVNIHIQGI
                     SFKKDKVDKEEAKATKKN"
     gene            26574..27080
                     /gene="nusB"
                     /locus_tag="ACOIAF_000711"
     CDS             26574..27080
                     /gene="nusB"
                     /locus_tag="ACOIAF_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HGZ2667737.1"
                     /translation="MKKDRAQKSTTREYIMKLIYQININKEDFETLEDKVDNFLKDNS
                     EHIINRYKELALQYSKNTNLKLEDTEIEDVIDKKYINTVCKALKENHDKIDELINKHA
                     KNWTVDRMPKVDVSILRLSVCEILYLDTPNKVSINEAVELAKIYCDDKSPKFINGILG
                     SVVDEIGK"
     gene            27064..28134
                     /locus_tag="ACOIAF_000712"
     CDS             27064..28134
                     /locus_tag="ACOIAF_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-sialoglycoprotein endopeptidase"
                     /protein_id="HGZ2667738.1"
                     /translation="MKLGNNIIIGIDTSCYTTSIAAISLDKKVIFNEKIMLEVRDNSK
                     GLRQSEAVFQHINNLGILSDRIKSFKDKFNVEGVCSSKKPRPVENSYMPVFNVGHNFG
                     KLLSSIYGCRFYETTHQENHIEASLLNSKLKNNNKFISVHMSGGTTEILLTSKQDSHH
                     NVCDTNLGKIAKISIKKDDKSKLYNNFGYNIDIIGGSKDISFGQLIDRVGIKLGYKFP
                     SGKYLDENALNCNLKIESGLKTSVRDGYMNLSGLENQVNKIINDNGDNTNQKEYISKL
                     VLDSVVRNMFKSLVYLCETYNVNEVIFAGGVSASKYILRELSMKLRKKHIEAYFTEPQ
                     YSTDNAVGCAIIGLNNFLGERV"
     gene            28131..29336
                     /gene="xseA"
                     /locus_tag="ACOIAF_000713"
     CDS             28131..29336
                     /gene="xseA"
                     /locus_tag="ACOIAF_000713"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HGZ2667739.1"
                     /translation="MKLRALDISEANSYIKRILINDPILSNLKVKGEISNFKVHSSGN
                     VYLSLKDETSKLNCVIFKSNFNRNLKLDNGVKIIANGYISVYERDGAYQLYINEIEIE
                     GIGNLHIEFNRLKEKLNKEGLFDPKYKIPIPKMPNSIGVITSPTGAVIRDIINVIKRR
                     YPKVNIKLYPVTVQGDKSAEEICEAIRFFNHMKNVDTLIVGRGGGSIEELWSFNDEMV
                     AREVFNSQIPIISAVGHETDFTICDFVSDMRAPTPSAAAEIATPSLDDINYKLGNIKS
                     RMSKSLTNQIELDQYRLETVFNKINNYLDSYTIKDKVIQLDKIYDKIIFGIENNLKLE
                     DEKLVKIGALLHNLSPLATMDRGYSITQKNGKVINSIKGLKIKDSIDIVLKDGNLECM
                     IDKIENKEG"
     gene            29338..29553
                     /gene="xseB"
                     /locus_tag="ACOIAF_000714"
     CDS             29338..29553
                     /gene="xseB"
                     /locus_tag="ACOIAF_000714"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HGZ2667740.1"
                     /translation="MNLTYEEAYKRLESILSELESKNASLDESLSLYEEGISLYKHCN
                     KLLDDAKLKISKFNQLGIEEDFKIEEE"
     gene            29556..30443
                     /locus_tag="ACOIAF_000715"
     CDS             29556..30443
                     /locus_tag="ACOIAF_000715"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HGZ2667741.1"
                     /translation="MEFKQCLKEKASFVEKVLKEYMPKEEGYQKTVIEAMNYSLSAGG
                     KRLRPILTLEACKIVGGNEDEAIPFAIAIEMIHTYSLIHDDLPALDNDDLRRGRPTNH
                     KVYGEAMGILAGDALLNYAFEVMLAGSINKENPEKYLKAINEIAKGAGIYGMIGGQVV
                     DVESENKQIEKEKLDYIHMNKTAAMMVGCMRAGATIGGANSEQMEEITKYAKNIGLSF
                     QIVDDILDIVGDEAKLGKKVGSDIENHKSTYPSLLGLDKSKEIAHNLIDEAKKSIEKL
                     SDDVDFLKGLAEYIIDREY"
     gene            30473..30910
                     /locus_tag="ACOIAF_000716"
     CDS             30473..30910
                     /locus_tag="ACOIAF_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HGZ2667742.1"
                     /translation="MDFFSEIFNNGALGISLIACFLAQFIKIFTGKEKRIELSRILIS
                     GGMPSSHSSFVTSLATVVGIEKGFNSTDFAIITVLALIIMYDAAGVRRAVGKQATILN
                     QMVADIQHGKHIEQKKLKELIGHTPLEVWFGALLGIVTALILM"
     gene            30944..32809
                     /gene="dxs"
                     /locus_tag="ACOIAF_000717"
     CDS             30944..32809
                     /gene="dxs"
                     /locus_tag="ACOIAF_000717"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HGZ2667743.1"
                     /translation="MYKYLDKVNSPKDIKNMSIEEMDLLAKDIRKFLVKSVSKTGGHL
                     ASNLGVVELTLALHKVFDSPKDKIVWDVGHQSYVHKIVTGRKDCFVSLRQFNGLSGFP
                     KENESPHDIFDTGHSSTSISIATGIACARDIKKENYSVISVIGDGSITGGMALEALNQ
                     LGYIDTNMIVILNDNEMSIDKNVGGMSKYLSSIIRNSTVEKMTDEVDKILNVTQTGEI
                     LSKTAHRFKDKLMYSFSPQDCSFFDSLGIRYYGPIDGHNTKELIDILRKAKHKKGPVL
                     LHVITKKGKGYRFAEEQPDKYHGVSKFDIKTGVTSAKVKSMSISVGEKLVDMANSNEN
                     IVAITAAMPSGTGLNLFESAYPKRYYDVGIAEQHATGFAAGLAKNGMKPYFAVYSSFL
                     QRAYDQVIHDVCITKKPVTFLIDRAGLVGNDGETHHGMFDLSYLNSIPNIVVMAPKDT
                     REMELMMDLSLKLDCPLAIRYPRGSSYYLDKGEYGEIVLGKYEVLDDGQDTVILCIGS
                     MVKHALEAKEILSREGINPTIVNARFLKPIDEGMLKALLKNHKNVVTIEDNIVTGGFG
                     SRINKFIIDNEYNVNILNIAIPEEFVKHGNIDELYDFVGLSPKSIADKIRKLVIE"
     gene            32942..33754
                     /locus_tag="ACOIAF_000718"
     CDS             32942..33754
                     /locus_tag="ACOIAF_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family rRNA
                     (cytidine-2'-O)-methyltransferase"
                     /protein_id="HGZ2667744.1"
                     /translation="MKKRIDLLLVEQGYFESRERAKKAIMAGLVFVDNQRCDKAGTEV
                     KEDCSIEVKGNPIPYVSRGGLKLEKAMKNFDLTIDGKVCMDIGASTGGFTDCMLKNGA
                     IKVFSIDVGYGQLAWKLRQDDRVVCMERTNIRNVTIEDTKQFADFASIDVSFISLKLV
                     LPKAKELVRHDGEVVALIKPQFEAGREKVGKKGVVREKSTHIEVIKMISDFSVENGFE
                     ILGLDFSPIKGPEGNIEYLIHLRNGNEGYEFDGETYNNKIVEVVEASHNLDK"
     gene            33774..35453
                     /gene="recN"
                     /locus_tag="ACOIAF_000719"
     CDS             33774..35453
                     /gene="recN"
                     /locus_tag="ACOIAF_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HGZ2667745.1"
                     /translation="MILELYMKNCALVEELRLNIDKNLNILTGETGSGKSIIIDALGL
                     CLGDKYDRSFLRKGTDKGLVEAVFFSDNRYLKKILEENDISMEDDNLLVITRLIYSDG
                     KSTARVNGRTVKVSLLKEIASTLIDIHGQHQNQALFNKDTHLKFLDLFGENELEEFKI
                     AYKKVYHKYSEVKKALNCLTENKDEMQIQREIDLLRFQINEIEAANLNKNEYEDLLKQ
                     REVYRNSEKIYNNLNSSYSKLHNGEYNVIDLIGLASKELNDISKYDSVLSEYSDTVER
                     IMYELQDISGEIRNYKDNIDFEPYELEQIELRIDEINNLRRKYGDSIDDIFEYYGKIK
                     DRLDEILNRDERVEQLRSKLMNIEEDLKIKASKLTKARIEVATTLEKILLDELKSLNM
                     KNVMFKVNFEKGPFTLDGVDDIEFMISFNLGEDIKPIYKVASGGEMSRFMLAFKTILA
                     DIDDIDTLVFDEIDTGISGIAAQIVGEKLSDIAKKKQIICITHLPQIAANADTHYCIE
                     KDTSNNRTFTNVSKLNESQRKNEIARLIAGNNITEKTIEHASEIIELAKKC"
     gene            35667..36533
                     /locus_tag="ACOIAF_000720"
     CDS             35667..36533
                     /locus_tag="ACOIAF_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667746.1"
                     /translation="MNKKDTMHLIFTIVIFILLWYIVVAVSEINKNIESCSNKLDYLS
                     QEIMSTKSDISNTINEEMSKSYITKSIDLKVKKIEKKECVIDVDVQLSKLGKNGKVFF
                     MYKEDSDKWNETEMTKHGELSYACEIKINTGSEYDYKVVTSGAISESSDVQKLTKSDY
                     MPEQPIFNSGIRDNREYFIEIVENSNLFNHESEKSKNKVYDNIRLEKVDIIVNEGKKD
                     TIYKAKLAEGLDDGKTNNSNRNQVNYEVSFPKRDIDDIIYIKAKLTYNNGVEYTKDIT
                     EDITMGLQDLEK"
     gene            complement(36626..37117)
                     /locus_tag="ACOIAF_000721"
     CDS             complement(36626..37117)
                     /locus_tag="ACOIAF_000721"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGZ2667747.1"
                     /translation="MADKMAQNITLQFVEEKDLENLENVKLLFTEYSNSLNIDLCFQD
                     FNNELKTLPGKYKKPSGSLILAFVDENLAGCVALKKLEGKICELKRLYVRNQFRGLKI
                     GKILLEEIIEEAKKFGYTHMRLDTLPSMKSAQGLYEKFGFYDIEPYTYNPIEGARYME
                     LKL"
     gene            complement(37178..37618)
                     /locus_tag="ACOIAF_000722"
     CDS             complement(37178..37618)
                     /locus_tag="ACOIAF_000722"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGZ2667748.1"
                     /translation="MKLNVRIADINNWHDLVSLSVDKSQLDYIESNALSIAESKFITA
                     WVPVGIYDENSLIGFAMYGRLEDDRIWLDRFMIDSKYQGKGYGKASLDFLVNHLKNEY
                     NCDELYLSIFEDNKMAIKLYKDFGFEFNGELDYGGEKVMVLKSN"
     gene            37779..38837
                     /locus_tag="ACOIAF_000723"
     CDS             37779..38837
                     /locus_tag="ACOIAF_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase S55 domain-containing protein"
                     /protein_id="HGZ2667749.1"
                     /translation="MNKINKNFKNIWIILGAVSIFTMVIIFNKNCNVNKETQIEAFTI
                     KNHDKKFVYPMGNIIGVKANTDGVLVLGYEEEDVDYIGGIQIGDNIVKIDNKRIKNSQ
                     DVSEILNKIKKSKVEVTFERKNKYKTEVIETKKENGKYKLGLWVRDKISGIGTMTFYD
                     PSMEKFKAIGHAIKDSDTNELLKIKQGYIYKPEQLKIVKASNEKVGKIKGDFNDSNLM
                     GNFSNNSELGISGNITENHNKEFNVANKEKQLIEVGRPQDVKIGDAVILFEDKNKNIT
                     SYDIKIESIVYDKGNYRDMVIKVVDDKLLEYTGGIVQGMSGAPIIQNNKIIGAITHVF
                     RDNPKKGYGIFIDEMIKL"
     gene            38979..39803
                     /gene="spo0A"
                     /locus_tag="ACOIAF_000724"
     CDS             38979..39803
                     /gene="spo0A"
                     /locus_tag="ACOIAF_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0042173 - regulation of sporulation
                     resulting in formation of a cellular spore [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA];
                     GO_process: GO:0051606 - detection of stimulus [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HGZ2667750.1"
                     /translation="MGGFLVEKIKIVLADDNKDFCQVLKEYLSNEDDIDILGIAKDGI
                     EALDLVKKTQPDLLILDVIMPHLDGLGVIEKLNTMDIPKMPKIIVLSAVGQDKITQSA
                     INLGADYYIVKPFDFVVFINRIRELVSNRVTQVEPKPRPVQETQMTRSDFVKNVGNIE
                     TEITNIIHEIGVPAHIKGYLYLREAIKMVIDNVELLGAVTKELYPSIAKKFNTTPSRV
                     ERAIRHAIEVAWSRGKVDTINQLFGYTVHNTKGKPTNSEFIAMIADKLRLEHSMVK"
     gene            40216..41337
                     /gene="steA"
                     /locus_tag="ACOIAF_000725"
     CDS             40216..41337
                     /gene="steA"
                     /locus_tag="ACOIAF_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cytokinetic ring protein SteA"
                     /protein_id="HGZ2667751.1"
                     /translation="MRVEAPIKVDRKTKKLAKRVESGEIAVINHIDIDEVAANSLVEA
                     KIKLVINAAPSISGRYPNKGPGILTENNILIIDNVGEELFEELKEGETIEVVDGKIYR
                     KGKFLGAGEVLDKYEVSHQIKAAYENLAVELDRFIDNTIDYAKKEKGFILGEVEIPKV
                     KTNYANKHVLIVVRGQDYKEDLSTMLSYIEEVKPVLVGVDGGADALIEFGYTPDVIVG
                     DMDSVSDEALKKASEIVVHAYTDGRAPGLKRVEELGLDAVVFPAPGTSEDIAMLIAYE
                     YKAELIVAVGTHSNMIDFLEKGRKGMASTFLVRLKIGSKLIDAKGVNLLYRSKLKIKY
                     IWALIATALFPVLVVASLSPGVQQFIQLMQLKFRVLLQM"
     gene            41355..42230
                     /locus_tag="ACOIAF_000726"
     CDS             41355..42230
                     /locus_tag="ACOIAF_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HGZ2667752.1"
                     /translation="MHINMKYYIVTIGAIFIALGIGMLVGFNLNNNQQLSEQQANIIN
                     DLDDKFNILKEKNDKLDSDLASVNRDYEEAVNFINKNVDKILAGSLNGKSIGIISANE
                     NDDYTKNIEDIINKSNGSIAFNIILKENITNPEKLKEMSTKLGTDVKNANDAVNYIID
                     TLKKEDASDILTYLQELDVIKFNFIGDTYLKYDSVVIAGGNDAKDSTKQFEKIEKFVV
                     SKLKSENKYLVEVQNTGVKTSYVELYSKNKVATIDNIDEGIGSISLAILLQQGNIVGN
                     FGRLDTATSLLPSIK"
     gene            42254..42946
                     /locus_tag="ACOIAF_000727"
     CDS             42254..42946
                     /locus_tag="ACOIAF_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HGZ2667753.1"
                     /translation="MDSYISIIIPAYNEESRIKYTLESILDIKQINEIIVVDDGSIDN
                     TAKVVSELENEKIKFFKLDKNRGKGYALNYGIKLAMKNADIIGFLDGDLGSSAKEVEK
                     LIVPILNGEADVTIAKFPPAKRKGGLGFVKGLAKNSVFEMTGVELDATLSGQRIFKRE
                     VLEKFDEIPFGYGVEVGMTIDILKYGFKIKEVLVNMTHNETGRNLKGFIHRGKQYYHI
                     KKVLGQKKKEWR"
     gene            42954..43778
                     /locus_tag="ACOIAF_000728"
     CDS             42954..43778
                     /locus_tag="ACOIAF_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HGZ2667754.1"
                     /translation="MKDYILYTILLLTGLFGTYAVIPLFKNLLINGNVLRPNYKKDMI
                     PVSMGIVFLPMIVINGIILAFFTTEFKDLAYIFMFMFGMISMFFAGILDDVIGNRDVS
                     GLKGHFKSLFKGSLTTGGFKALFGGFVGLIVSVAISKDIIDIIVNTLIIALSTNLMNL
                     LDLRPGRAIKAYLFIMVIIFLTLTGFVQVLPLLIVPNVLAYFNYDLKARAMMGDTGSN
                     VLGISIGMLISFGYPFNIRLGWLIFVVIMHIFTEKYSLTKIIEKNKLLNFIDKLGR"
     gene            43783..44850
                     /locus_tag="ACOIAF_000729"
     CDS             43783..44850
                     /locus_tag="ACOIAF_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HGZ2667755.1"
                     /translation="MISKRVGIVESIVSQTETLDDIRVNINGEIQRAYNYPKISGTIN
                     IGDEVVLNTTAVELSLGTGGYHFVITNLNNIESTLTEGGHIMKLRYTPLQIKVDSVEE
                     QESIYHDKFANFKGLEGLSVVVGTLHSMLTPFAASFKRNNPNKKLVYIMTDGASLPIY
                     LSKNVDTLKEKKLIDSTITIGNAFGGDYECINIYTALITAKEILKADAVFVSMGPGIA
                     GTGTKYGFTGIEQGSILDAVKKLEGRAIAIPRISFADKRERHQGISHHSMTVFKEIVN
                     VNVDIPITIYDDEKLNFIKEQINLNGLDKKHNIIYIDNNKTKEDLDYFNLKVKSMGRN
                     YEQDEAFFKAASTAAYYLMEV"
     gene            44852..45388
                     /locus_tag="ACOIAF_000730"
     CDS             44852..45388
                     /locus_tag="ACOIAF_000730"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HGZ2667756.1"
                     /translation="MVLEEKTISSDRVYTGKVITLKVDTVEIPGQGYQKRELVEVGGA
                     VGIVAITDDNKVVLVKQFRKPIEKPIFEIPAGKLEKNESPKECAERELKEETGYSAKN
                     IKLIHKFFTSAGFSNEIMFVYLATGLTPGENNLDADEFLDVYEIELEEAYNMVLKNDV
                     EDAKTSIGLLLVKDMFKN"
     gene            45456..46061
                     /locus_tag="ACOIAF_000731"
     CDS             45456..46061
                     /locus_tag="ACOIAF_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HGZ2667757.1"
                     /translation="MRRTYRKNDFRELNRQIIIIGLLFMMSIVVGSYINKILPGSSNN
                     ILNNINPAVEYYNLNISIKDTVIQNLKSDAIFMGSIALLSLFVVTIPAVLVAFVLKGM
                     SIGYTINSCILALKFKSIKMILIILFKNLIIIPCAIILALISLSYFKEMVYEFKKKNR
                     KNMQFLIKRYILNIIIIIALSLGLQLILNTASIGIIKFLAK"
     gene            46073..46987
                     /locus_tag="ACOIAF_000732"
     CDS             46073..46987
                     /locus_tag="ACOIAF_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGZ2667758.1"
                     /translation="MDIIEGYIDYIKDKKKLSENTVASYFMDIKKYMEYINQKSIKLV
                     DIVENDIISYLIKLEKDNVSIATIARMISSIKSFHDYLFLNHICTNNPAKDIKKPKIK
                     KENINILTEEEIERLLNFPKLTTPKLIRDKAIFEVLYGTGIKVSELVEMNIEDIDLDI
                     DYIYCNSCCKNQRVIPLCDITKLYLEKYLKEARPKMAVEGEKSLFVSSLGQRFTRQGL
                     WKVIKKYSNLANIDKNINPTMLRHSFAIHLLNEGANIAVVSKILGNVNLSSLQVYLNH
                     IDKNVRREIKEKHPRNDVDVELQKAEAK"
     gene            47029..48201
                     /locus_tag="ACOIAF_000733"
     CDS             47029..48201
                     /locus_tag="ACOIAF_000733"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HGZ2667759.1"
                     /translation="MSRVIWIVIDSVGIGALPDAEKFGDSKDVSTLGNIFKEYPDIQI
                     PNMRNLGIGNIDGIDFFESIKEPIGCFGKCKEMSQGKDTTTGHWEMTGIIVDKPFKTF
                     EHGFSKEIIEEFEKKTGRKVVGNKPASGTVIIDEYGEHQIKTGDVIVYTSADSVFQIA
                     ANEEVIPLEELYNMCKIAREIMMGDNAVARVIARPFIGKKKGEFVRTSNRRDYSLDPF
                     EPTVLDNIKESGLDVLAVGKIEDIFNGKGITDAIHTKSNMDGVDETLNYMKQDNKGLI
                     YSNLVDFDSKYGHRRDPEGYKKALEEFDSRLPEIMANMREDDILIINADHGNDPTYKG
                     TDHTREYIPVMIYGNKIKKGFNLGVKDTFADIGATVADILNVKLPKHGSSFKEDLF"
     gene            48248..49060
                     /locus_tag="ACOIAF_000734"
     CDS             48248..49060
                     /locus_tag="ACOIAF_000734"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HGZ2667760.1"
                     /translation="MFEKIAQSSKFINSKSNIKPKIGLILGSGLGDLANDIEDPVTIK
                     YSEIPNFPVSTVAGHAGQLVIGKLEGKEVIAMQGRFHYYEGYSQKEATFPIRVMKELG
                     VEILIVTNAAGGVNKEFKAGDLMLIRDHINFSGSNPLVGKNDDRFGARFPDMSDAYSS
                     KYVDVVKNCAKECNIDVKEGVYMFFSGPNYETPAEVRMAQILGADAVGMSTVPEVIVA
                     SHSNIGVIGISCITNMAAGILNQPLSHEEVIETTQKVKSEFMNLVKTTVKNL"
BASE COUNT        19809 a         4968 c         9094 g        15253 t
ORIGIN      
        1 catcctaaaa gcatgtgtac ataaataaag taagactatt ttatcttata taataaagag
       61 ttaataaaat gatttttata aatatagtta ttttaaaaac tactttacat atattggatg
      121 agtataaaaa tccatagggc cttttactaa atctttattg tttttataat atattgaatt
      181 atatatattt gtagctttta tatcataact tatgagtgtt ttaaaaatcg tatcatctaa
      241 tgaaaatgat atatttgaaa acttgtttat tatttttatt tcagatttat tttttatctc
      301 ttttagaatt tctctacctt tatcattaaa agcaagtatt cttatataag gtatagtatc
      361 tatgtttttt ataagttgca tgtcgctttt tttaatacct aacaatatat tgtttagagt
      421 tcgtttgatt ttggttaaag tgtatcgctt ggatttaatt gctaattgta gttcatgtag
      481 aaattcagat gtaaagatat ctttatagat tctgttttct atgccttcat ttacttcaaa
      541 atactctttt aaagtgtcca tatctctaag tataattgtt tttaaaatat caaagaagaa
      601 gtcatcaaac ataggtaaaa aaccattatc tattttttca tttaggatgt tataagtttt
      661 ttctggaata acatttttta aatgaaaaag gtctgtattt tctttaagtg aagttcttat
      721 agcagtagca gaacaaatat cactctcaat ttcttcggaa ttatattctg attggaccct
      781 ggagatagta aatggtttta tactactgtt taaatggatg aggtttttta tgtattctat
      841 acccagtatg ttgtttgatg aatttaatgt ttttgataat tgttcctttg aataattttg
      901 tataaaatta ttttttttgt cttgatgtat ataatcaaaa agtgcattag ctctagctgt
      961 tggaaataac agaccatcat ctaaatattt ttttaaaagt aacttaaatt tatttggttc
     1021 atttgctaaa atttcagcta tattataaag aatttctgta tttccttctt cactaccaaa
     1081 acatatagaa tttatgcagt ttagtgaatt taaaattgtt atagcaccat gagaaaaaat
     1141 ttcagcactt tgacaagcaa ataaagttgg taattctata actaagtcta caccattttc
     1201 gacagcaatt tttgctctag tgtatttatc aaataaagct ggttcgcctc tttgtagaaa
     1261 atttccactc attattgcta cagtatgtgt tgcatttgtt ttttctatag atttagataa
     1321 atgatatatg tgtccattat gaaatgggtt atattcaact attaatccaa gtatgttcat
     1381 aaaatactcc ttttatcttt aattatttta tatatatcac aatttattat aatatgtata
     1441 aaatttaaat ttttaattat aaaattaaaa aacgtaaaaa aatttataaa aatgtagaaa
     1501 tgttacctac ataaaaaact ggttaaatgt tattaaaata actttattag ctctttatat
     1561 tatattatta taacaaatgg gaaagaataa tctacattaa gttaacttgt taatatatta
     1621 ttagtgaaat acacaattta ccatactaga aatatgatat aatataaaaa aaaagtatat
     1681 acttttatat taaaaggtga tatattattt atgaaaatcg tttaatgtat acaataatat
     1741 atctataaca aaataataaa agatagagtt ttatttagac atttattaca aatcaaaatt
     1801 tattacatat tatttataaa aaaatctaag gaggaaataa taatgaaaat attagtatta
     1861 aactgtggga gttcttcatt aaaatatcaa ttaatagata tgaataatga agaagtttta
     1921 tgtataggat tggttgaaag aataggaata gaaggttcta tattaaaaca tgaaaaagca
     1981 ggtagagatg ataaatacgt tgttgaacaa ccaatgaagg accacaaaga tgcaatagca
     2041 ttagttttag aagctgttgc tcatccagaa tttggtgcag ttaaagaaat gaaagaaata
     2101 gatgcagtag gacatagagt tgtacatgct ggagaaaaat ttgcaacttc agtagtaata
     2161 actccagaag tagaagaagc tttaaaagaa tgtatagatt tagctccact tcataatcca
     2221 gcaaacataa tgggaataga tgcttgtaaa gctatacttc cagatgtacc aatggtaggt
     2281 gtatttgata ctgctttcca tcaaacaatg cctaaatctt catacttata tggtttacct
     2341 catgagctat atactaaata tggggtaaga agatatggat tccacggaac ttctcataat
     2401 tatgtatctc aaagagcagc agagatatta ggaaaagata taaaagattt aaaaatagta
     2461 acttgtcact taggaaatgg tgcttctata gctgctgttg atggtggaaa atgtgtagat
     2521 acatctatgg gattcactcc attagaagga ttaataatgg gaactagatg tggagatata
     2581 gacccagcta tattaccatt cttaatgaga aaagaaggtt tagatgctga tggattggat
     2641 aaattaatga ataaagaatc tggagtatat ggaatgactg gaatttctag tgacttcaga
     2701 gatatagaag atgcagctaa aaatggagac gaaagagctc aagctacatt agaagcgtat
     2761 gttaaaaaag tacaaaaata tataggcgct tatgctgctg aaatgaatgg attggatgtt
     2821 gtagtattta ctgcaggcgt tggtgaaaat ggaaaggcta taagagcaga tattgcttct
     2881 aacatggagt ttttaggaat gaaattagat aaagaagcta atgatgttag aggaaaagaa
     2941 acagtaatat ctactgctga ttctaaagta aaaatgcttt taataccaac taatgaagaa
     3001 ttaatgatag ctagagatac attaagattg gtaaaataat taaattaaac ctatgcctat
     3061 caagtaaaaa tacttgacaa acaaatgcta gatttgtata ataaatttgg tgatatgttg
     3121 aggtgaactt aatgaaagtt agcatagaaa aaataaatag aaaagaaact gacaaaatag
     3181 acttgaattt ttgtgaaaaa attgatacta ttagttattg tgatgagatt tacaaattag
     3241 tatcacctgt aaatttaaaa gggaaagtgt caaagactaa taaaggtttg tatctagata
     3301 tagatgtcaa ttttacaatt gttgataatt gttcaagatg tcttaaagaa gtagaaatac
     3361 cactagagta ttctatacaa ggttttttag taaaagaaga agattatgat gaagatgagt
     3421 ttgaggaatt tgatcctttt atattcgatg gtgaagaaat cgaccttatt gatataatag
     3481 aacagacatt agattttaat gtacctcata aagttctttg tagtgaaaac tgtaaaggtc
     3541 tttgtcaagt atgcggggca aacttaaatg aagaagagtg ttcttgcagt gaaattacaa
     3601 acgatgagga atacatagat cctcgttttg ctaaattaaa agatttattt aattaaattt
     3661 tgactaagga ggtggcgtaa atggcagtac caaagcgtaa aacgtctaaa tcaaacacaa
     3721 aaatgagaag agcagctaac tcaaagatgg aagcaacagg atttgtaagt tgcccacaat
     3781 gtcatgagcc aaaattacca catagagtgt gtccagactg tggatattat aaaggtaaag
     3841 aagttgtgtc taagtaatag cttctaaaaa gcatgtagag tatactctac atgttttttt
     3901 tgtacatata aaaaaataag atgatttact agagaaaata agtggatttt atgcatatta
     3961 aaaaatagaa aaataataat ataagtattg aaaatttttg tgtgttacta tatactacta
     4021 ttagtagctg gtactaatta cagctataaa atttggaggg aacatgaaaa agaaaagtaa
     4081 agcccaaaga caaaaagagc ttatagatat gctaaagaca gatccttttt atactgatga
     4141 agaattatca agtctgtttg atgtaagtat acagaccata agattagata gaatgagtct
     4201 aaacatacca gagctaagag aaagagtcaa atcaatagca gaaactcaga gttcaaaagt
     4261 aaagacttta ggagtaaagg aaattacagg agaaatcatt gatttatctg tagggcgatt
     4321 aggtatatct atgttagaag ttacacagga catgatttac tctaagacta atactttaaa
     4381 agatacgtat attttttctc tagcagattc attggcaatg gctataatag atgcacctaa
     4441 ggttattatg agagttgcaa atgtaaaaag ttttaagttg atagaacaac aagataggct
     4501 tatagcaaaa gcagaagtgt atagaaatat agataaaaag cattatgtaa aagttgttat
     4561 caacaataaa gcccaagagc aaatatttag aggaaagttt atctttgaag aattagatta
     4621 ggagggaaat tatgaaaata gtaattgatg gaatgggtgg agataatgca cctaaatcta
     4681 atgtagaagg cgcagtaaat gctataaaag aatatcaggt tgatttaata atcacaggag
     4741 ataaagattt acttgaaaag gaattttcaa attatgaatt tgatagaaat aaattagaga
     4801 tagttcatac tactgagatt atagaaaacg aagataaacc agtaaaggct ataagaagta
     4861 aaaaagattc atctatggta gtagcattaa atcttgtaaa agaaggaaag gcagatgcta
     4921 taatatcagc tggaaataca ggagccttac ttgctggtgg attatttgtt gttggtagaa
     4981 taaaaggaat cgatagacct tgcttatgtt cagcaatacc aaatgtaaag cgaggaatga
     5041 cacttatagc agactgtggc gcaaatgcag attgtaaacc taagaatcta gttgaatttg
     5101 cagctatgag taatatttat tcaagaaaag ttttaggtct tgaaaatcct aaggtagcat
     5161 tagcaaatgt tggactagaa gaaggcaaag gcaatgattt agtcaaaagg tcatatgaag
     5221 aaattaaaaa attagattta aattttatag ggaatgtaga agctagagaa gttataaatg
     5281 catacacaga tataatcata tgtgatggat ttacaggtaa tatattactt aaatctgctg
     5341 aaggtgtagc actttcagtt atgagtctta taaaggaaac ttttatggcc agtacaaaaa
     5401 gtaaaatagg agcattactt ataaaggatg atttaagaaa attaaaaagt tttatagatt
     5461 attctgagta tgggggagct ccacttttag gtctaaatgg aggagttata aaagctcatg
     5521 gtagttctga tgctaaagct ataaaaaatg ctataaatca aggaataaaa ttttcaaagg
     5581 gaaaagtcgt tgaagatatt aaccaattta taagcaaata caatgaagaa aataaaaata
     5641 acgaagatga ataaactcgt tttggaggtt taatatataa tgaatacaaa ggctggaata
     5701 ttaggagtag gaagttatct acctgagcaa tcatatgata attttcactt cgaaaaaata
     5761 atggatacat cagatgagtg gataagtact agaactggta ttaaggaaag aagatttgcc
     5821 aaagagtctg aagcaacttc tgacttagca tctaaagctg ccttaaaagc tatagaatgt
     5881 gcaaagttaa acgtagagga tatagagctg attatattag ctacaattac tcctgatatg
     5941 tctttgcctt caactgcatg tatagtacaa gatgcaatag gagcagtgaa tgctactgca
     6001 tttgatatat cagcagcttg ttctggattt gtatatggag taactattgc aaagcaattt
     6061 gtggaaacag gttgctataa aaatgtgctt gtaataggag cagaaacttg ttctaagttt
     6121 ttaaattatg atgatagaac aacagcagta ttgtttggag atggagctgg agcagctgtt
     6181 attggccctg taaatgaggg tggaatactg tctacacata tgggctctga tggtaaagga
     6241 aaagattgtt taaaagtacc tgctggaggt tcaagactta aggcaagtaa agagactgta
     6301 gaagctaatc tacatacaat agaaatggca ggtagtgatg tgtttaaatt tgcagttaga
     6361 aagatggcag aaacttcttt aagagctctt gaaaaagcaa atctaaatac aactgatata
     6421 gactacttag tgccacatca agctaatata agaatcatac aagcttcttc taagagatta
     6481 gagcttgata tgaaaaaagt ttatgtaaat atagacaaat atggaaatat gtctgctgca
     6541 tctataccag tagcattaga tgaagcttat agagaaggta aaattaaaaa gggtgataat
     6601 gtagttttag ttggctttgg tggaggtcta acttgggggg cttcagttgt caagtggact
     6661 ttatagtata aaatatctaa aatgatttaa ttaacaaaat taaacttata cacaaaataa
     6721 agttgattaa taataaaaag tttactttta tgatttcaag tgaaggagaa ttgtatgaat
     6781 aaaatttgca aaatattaaa tataaaatac ccagttatcc aagggggaat ggcatgggta
     6841 gctactgcat cattagcaag tgctgtatct aatgcaggag gacttggcat aatagcagca
     6901 ggaaacgcac caaaagaagc tataaagaaa gaaattgttg agtgtaaaaa attaacagat
     6961 aaaccttttg gagtaaatgt aatgcttatg tcgccatttg ttgatgatat aattgatttg
     7021 attatagaag aaaaagttca agttattact actggtgctg gaaatcctgc aaagtatatg
     7081 gatagattaa aggaagctgg aacaaaggtt attcctgtag tacctacaat agctttggca
     7141 caaagaatgg aaaagctagg agctacagca gtaatagcag aaggtactga aggtggagga
     7201 catataggag aacttactac tatggtctta gttccacaag ttgctgatgc tgtaaacata
     7261 cctgtaatag ctgctggagg aattgtagat ggtagaggaa ttgcagcatc atttgcatta
     7321 ggtgccagtg cagttcaagt aggaactaga tttatttgca gtgaagagtg ttctgtccat
     7381 tcaaactata aaaacttagt actaaaagca aaagatagag atgcaattgt aacaggaaga
     7441 agtactggtc atccagtaag aacattaaaa aataaactat caaaagaatt tttaaagatg
     7501 gaacaaaatg gagctactcc tgaagaattg gataaaaaag gtacaggagc tttaagattt
     7561 gcaacagtag atggagacat agaaaaaggt tcatttatgg caggtcaaag tgctgctatg
     7621 gtaaaagaaa taacaccttg taaggaaatt atagaggcta tggtaaatca agcaagagag
     7681 attatgccag caatagaact gtaaatcagg aggagcaaga tgggaaaagt tgcattagta
     7741 ttccctggtc aaggagctca atatgtaggt atggccaagg atttatatga aaataatgct
     7801 gttgctaaaa gtgtaataga tgaggctagt gatgccttaa atatggattt aagaaacctt
     7861 atgtttaatg gaaatgaaga agaactttct aaaactgaaa acactcaacc tgctatggtt
     7921 acacatagtg tagctgtatt aaaagcagta caagcccaag tagatttaaa atatagtgca
     7981 tgtttaggtt tatctttagg agagtatagt gcattggttg cagcagatgc aattgacttt
     8041 aaagatgctg tatgcttggt caaaaaaaga ggtaagttta tgcaggatac agttccaaca
     8101 ggtgttggag ctatggcagc aattttaggt ctagatagaa gtatattaga aaatgtagtt
     8161 aaagatatac aaggaggtat tgtggaagta gcaaattata attctcctgg tcaaatagtt
     8221 atatctggtg aaaatgaaaa gatagaagaa gcaatggtga aatgcaaaga agcaggagca
     8281 aaaagagctg ttaaattaaa tgtaagtgga ccttttcact catcaatgct aagagaagct
     8341 ggagtaaaat taggtagtga gctagaaaaa gtcaacataa ctaaacctaa aattgatgta
     8401 gtagcaaatg taaatgcaga ttactataaa agtgaaggga aagaactgct tataaaacaa
     8461 gtaagttcat ctgttctttg ggaagattct atagaaagac ttttaaatga tggatatgat
     8521 acttttatag aaatgggacc tggaaaaact ttaaaagcat ttattaagaa gatagcttcc
     8581 aataaaaaag caaatgtcaa tatttataat atagatggta tagattctct taatgaattt
     8641 gtacaaaatt atagaaatgg agagatataa tgataaatct tacaggacaa gtagcagttg
     8701 ttactggtgg ctctaggggt ataggaaaag aaatagcaaa aaaactagca tcttttgggg
     8761 ctgatgtagt aatcaattat acttctaaag aagatgaagc actaaaaact aaaaatgaaa
     8821 tagaaagtat gggggtaaag tgtacctcta taaaatgtga tgtgtctaaa tttgatgaag
     8881 tgaatcaaat gatagattct gttgtaagcg aatttggaaa aattgatata ttggttaata
     8941 atgcaggcat aactaaagat ggtctgctta tgagaatgaa agaagaagat tttgatagag
     9001 ttatagatat aaacttaaaa ggtgtcttta attgtacaaa agcagttact aaacctatga
     9061 tgaaaaagaa gtatggaaga ataataaata tgacttcagt agttggaatt atgggtaatg
     9121 cagggcaaac taattattgt gcatcaaaag caggtgtaat tggatttaca aaagcttctg
     9181 caagagagtt agcatcaaga aacataaata taaatgcagt agcacctgga tttatagaaa
     9241 cagatatgac taaagtacta agtgatgatg taaaagaatc aacactagca aacataccaa
     9301 agaaatctta tggtaaacca gaagatgtag ccaatgccgt agcattttta gttagtgaca
     9361 tgtcaagtta tataacagga caagtaataa atgtagatgg tggaatggta atgcaataat
     9421 aatttaaaaa gtagagaaat ctattaaaat aaaaaatttt aggaggtaca caatgtttaa
     9481 taaagttgta gaaataataa tagagcaatt aggagtagaa gataaagaaa taactatgga
     9541 aacatcatta atgaaggatt tagaagcaga ttcattagat gcagtagaaa taataatggc
     9601 acttgaagat gaatttggta tagaaatacc agatacagaa gcagaaaact tcaaatctat
     9661 aggagatata gttaagtata tagaagctaa taaatagtcg agctttaaaa aagtggaggt
     9721 atccgatgaa taaaagagta gtaataacag gacttggatg tgttacgccg ttgggaacag
     9781 gaaaagaaga gttctggagt aatataaaat ctggagtatc tggtatagat aaaataacta
     9841 attttgatgc tagcacatat caaactcaga tagcaggaga agttaagaat tttcatccag
     9901 aagaatatat aagtaaaaaa gaattgaaga gattagataa atttgctcaa tttgcaatag
     9961 tatctgctaa attagcagtt gaagatgcaa atcttgattt agataaagtt gatagagaac
    10021 gttttggtgt tataataggt tctggaatag gtggagttga agctatagaa actcaacata
    10081 aaattctttt ggaaaaagga aataaaagag taagttcact ttttgtacct atgatgatag
    10141 gaaatatggc agctggtcaa gtttcaatat ttttaggagc aaaagggcct aacactaatg
    10201 tatgtacagc ttgtgcatca ggaactcatt caataggtga tgcttttaag gtaattcaaa
    10261 gaggtgatgc agacatcatg gtagcagggg gctctgaagc tgctgtaaca ggtcttgctt
    10321 ttgctggatt ctgtaacatg aaggctatgt caactagaaa tgatgaccct aaaactgcat
    10381 caagaccatt tgataaagac agagatggtt ttgtaatggg agaaggagca ggtattgtta
    10441 tcttagaaga cttagaacat gctttagcaa gaggcgctaa aatatatgca gaagtagttg
    10501 gatatggttt aactgctgat gcataccata tgacaactcc agctgaaaat ggagagggag
    10561 cagctaggtc tatgaatatg gctttgaaag atggaaatgt tccattagaa gaagtagatt
    10621 atataaatgc tcatggaact tcaacttatt acaatgattt atatgaaact atggctataa
    10681 aaactgtatt tggagaaaag gcatatgact tatgtgtgtc atcaacaaaa tctatgactg
    10741 gtcacttatt aggagcatca ggtgctatag aggctgttgt ttgtgctatg agtatagaag
    10801 atagttttgt gcctccaact ataaacatac aagaagttgg tgaagatttg gatttagatt
    10861 atgttccaaa tcaaggtaaa gaaaaaaata ttagatatgc actatcaaat tctttaggat
    10921 ttggtgggca taatgctaca atagtactaa aaaaatatgt ataaataaat attaaatata
    10981 aaactaaaaa acaagcactt gttttaactg tataaattta tattagctta aatttaaagt
    11041 ctcctaaatt attttaggag acttttttat taagatttaa gtctaggata tgttgtaaat
    11101 cattttgata tattgtatta ttaattatta agaggttatt tatatatgca agttttcatt
    11161 ctacaatata caaaaggagg actaaaattt tgaattttag actaccaaag aaaaggttac
    11221 ccaaaaagat aatttttata tgtataatac ttattagtat tattttgttt ataaagtatg
    11281 accaaaatgg attgaaagaa agagaaatta taaataaggg aaaatatatg tctgaaagcc
    11341 ttgtggcaga taaggagtat gatgaagcaa aaaaagtggt taagacttct tttgaatcta
    11401 ttccagaaaa aaaatacaat aaagtatata atgaagtttg ggatatgtta aagactattt
    11461 catatgttcc agatggaacg aagatagtca ttgattcttt agaaaagatg agagaatcag
    11521 atgtgaaatt atctgatgaa tgtagattta atatagaaaa aagattagca tcagtgtata
    11581 taatggataa taattattct aaggcagtag atttaactgt aaagtctata aagttggctg
    11641 agaaattggg gaataattat gaaaaagcaa gactagatgt tgatttggcg agcatatttt
    11701 taaagatagg tggctatgaa acaggagaga aattaataaa ggattctttt aaaatagata
    11761 ttgatgaagg tgaaaaaaat ggaatggtta gattatatgc tattataaat ctagcagaaa
    11821 tatatgtaga agagggagaa tatgataaag ctattgaaat aagtagcagg ataaaagatt
    11881 ataaaaagtt tgtaaatacg gataattaca atgattttga tataatggcc tctataatgc
    11941 agtcaaatgc gtatgtgggc aaaaataata tacaaaaggc aaagactttt ttagaaaaag
    12001 ctgattatct tataaaatca gatagaacag tttatatatt agataaagat atgtactatt
    12061 atatggcaat gggaaactta gaatataaaa gcgaaaatta taattctgca atagataatt
    12121 ataaaaaaag tttagagata tctaccaaaa gaaagttaac ccaggagaaa ataaaaaatt
    12181 taaataagat attaaatata tatgaaaaac aaggaaatat agaatcttta aatgagtatc
    12241 aaaagatgtt aataaatgaa tataaagaga acaaaagtat aagagattca gagactagtt
    12301 tctatataat tgataaagta tcaaacgaaa gtaagctatt tgaaaaaact aaaaaagaaa
    12361 taaaatatta taaaatatta tttaccgtaa ttttaattgc tatattaggg tctacttttt
    12421 tatacaaccg attgaagtat tttaaagcac aaaatttaca tgatggtctt acaaatgttt
    12481 ataatagaaa aagttttgat attatgtatg aaaagtacag agagaaagat gataattttg
    12541 cacttgtgat gatagatatt gataatttca agctcataaa tgataattat ggacataaat
    12601 ttggtgacac tgttataaag ggaataacaa ataccatttg tgttatgtta gaacaagatg
    12661 ataaagtctt taggtacgga ggagaagaat tttctgtatt aataagaaat aaatctggtg
    12721 aggaagtagc agatataatt gacagtataa gggttgcaat tgcaaataaa aagtggaatg
    12781 atgatgtgac tgtgactata agtgcaggtg tagctcatat ttcagatagc aaagatgtac
    12841 tagaagaagc tgataaaaat ttatataaag caaaacaact tggtagaaat aaagtagtat
    12901 ataagtaatt atatactata taaataagag gtataaatat gaaacaatca atagctatag
    12961 taactgataa aataactaaa ctatcatctt tcttagaaga aaatatacgt ttggttttag
    13021 gtaattatat tgatataaac cactattatc tggaagtttt aagagagaat gataaaatac
    13081 aaggtgattt tgtactagta atgaatgacg atagattaaa cagtatgaga aagcatttag
    13141 tacaagatag taagattata gtgataagga gaacacttaa agaaaatgaa atatatggat
    13201 tattttcaat accaaatgga actgaagtat tggttgtaaa tgatacaaaa gaaactacct
    13261 tagaaacgat aagcttattt tacaaaatag gagtaaaaaa tttaaagttt actccatata
    13321 ttgaaggaaa aatttataaa aatatagaca tagctataac gcctggagta aagggaaaag
    13381 taccaagtta tataaataaa gtaatcgacc ttggaaatag atatattgat atatctacct
    13441 ttatagaaat aataaatggt cttagaatag atgttaaaga aatacgtaaa aatttaatgg
    13501 catattctga gagtttaata agtttagata atggagttaa agataattat aggcaactgt
    13561 ttttaaaaat agaagaacaa gatgttatat tgaatctatc aaaagatgga ataatattta
    13621 cttcagcaga tggtataata aacacattta ataaagaagc tttaaaaatt ttaggtataa
    13681 atgaaaatcc acaaggcaaa aacatagaag atatcatttc tgaagattta aatattttac
    13741 ttgaagacaa agaaataatt gatgaagtgg tatttacaaa taaaaaatat ataaatgtaa
    13801 ataaaaaaaa tattttttct atggggaata aagctgggat atattataac ttacaagaaa
    13861 taacgtatat aaaaaaatta gaacaaaatc ttactaataa attaagagaa caaggtcaga
    13921 tagcaagata tacatttaaa gatataaaaa ctaaaaataa aaatatggta aagtgtgtag
    13981 aattggctaa aaaaatatct aaatcagacc tttctgtatt aataatagga gaaagtggta
    14041 ctggtaaaga attattatca caatcaattc ataatgcttc caatagaagt aatcaaccgt
    14101 ttatagcagt taattgtgca gcagttccag atagtctatt agaaagtcaa ctgtttggat
    14161 atgaaaaagg ctcttttaca ggagctttaa gagaaggaaa aaagggtcta tttgaacttg
    14221 ctaataatgg aactatattt ctggatgaaa ttggagatat gcctccattg ctacagacaa
    14281 agttactaag ggtattacaa gaaaagcagg ttatgcctat aggttcccat aatgttataa
    14341 acatagatgt tagggtcata gcagcaacaa acaaaaactt actaaagatg attagagaag
    14401 gaaaatttag agaagacctg tattatagac taaatgtatt gccaatagat gtaccatcat
    14461 tgaggaatag aaaagaagat ataataacac ttatgaattt ctttttgaaa aacaatataa
    14521 gactttcgcc agaagttata actattttag aaaaatataa ttggccagga aatataagag
    14581 agttacaaaa tgttgcttct tatgtaagtc taatgtgtga tagtatggtt tttagagatg
    14641 acttaccacc atatataagg gtagaatgtg ctaacaatta tgaagatgga tttggttata
    14701 caagaaaaga atttgaggaa atactgagga ttctatatga aagaagagag tcatcctctg
    14761 gaattggaag agggtttata atcgaaaagc ttatacagga aggtattgag atttctgaaa
    14821 gcaaagttag aaaggtgcta tcttatttaa gtgaatgtgg atacatatca tctaaatctg
    14881 gtagaggagg aagtcaaata acttcagagg ggataaaact ttataataat ataaattaag
    14941 ctaagtattt gatattgaaa tcacatattt tgaatttaga tatttaaata ataataaact
    15001 tttatattta tagagattaa tttatattat gtcagaaaga atattgtaat ttataattaa
    15061 atattaatag gtttttaaaa taggattaga taggtaatag gttaagttag aataacccat
    15121 tacctatttt tatttgtaag aaaagtgtac atagaattag aagtaaaaaa tacaactaat
    15181 aaaactattg aaaatactag ataaataata aataaaaata attatagatt ttggcataca
    15241 tattgctaat tatagagata agacgacaaa ataattggga ggtttaatga tgaaatacaa
    15301 aacaacaggg acttgtgcta cagaaattga atttgaagta aaagaaaata aagttacaaa
    15361 tgtaaacttt ataggtggat gtgatggaaa tttaaaaggg ctaaaagtat tagttgaagg
    15421 catgaatata gaagatgtaa tacaaaagtt aaaaggaata gagtgtaaaa caaagccaac
    15481 atcttgccca gaccaactgt ctctagcatt ggaaaattat atgaatataa agtaatacat
    15541 aaaacaatcg attagttagg agtttaatta tgaaaccaat aatagggatt ttaggaaatt
    15601 taataataat ggaaaatggt atgtttccag gtcttgaaag gtcatatgta aataatgatt
    15661 atataaatgc agtgctaaaa ggtggcggta gtccagtaat aattcctgta aatacagata
    15721 aagaagttat aaaaaaacag attgaaatgg ttgatggagt cttaatatct gggggatggg
    15781 atatcaatcc tcaactgtat ggagaagaaa caagagaaga aacgacattt atatatccag
    15841 aagtggatga atttgattta attgcaataa gtatagcatt agaacttaaa aagcctatat
    15901 taggtgtatg taggggacta caaattttaa atgtaagttt aggaggaact ttatatcaag
    15961 ataataatct tatagaagga agttatataa aacatacaca atcttctaaa agacatgtag
    16021 ctactcataa agttgatgtt aaagaaggaa gtattttaga gggaatttta ggaaaacaat
    16081 tacttacaaa tagttatcat catcaaagtg tcaatcagct tggaaaagga ttaaaagcca
    16141 tagcatactc aaaagatggt ataatagaag ctattgagaa agaggatgaa aattttgtag
    16201 taggagtaca gtggcatcca gaaatgatgg tagattactg tgataaaatg gaaaaactat
    16261 ttaaatattt tataaatatt tgttcgaatt tataatatac cttgggggga ttgagcatga
    16321 acgaaaagaa caaaatggga ttaataagta ttatactttt aggtattaat gcagtagtag
    16381 gagctggagt ttttctatta ccaggagatg caatgaaatc atttggtgta gcaagtatat
    16441 ttgtatatat atttgatatg ttgttagttt tatctatggc attctgtttt gctgaggttg
    16501 caggtaaatt taataaaaat ggtgctgcct atgtatatac aaaagaagct tttggagatt
    16561 tttgtggttt tgaagtaggt cttatgaaat gggtaatagg atgtatttct tggggagctt
    16621 taatagtagg ttttcctaca tcattatcag cagtatgggc tccagcaggt gaaccacata
    16681 tacaaaaaat tataattgta gccatgatag ttgggcttac aattataaac ttattaggtg
    16741 tatctctatc aaaaattgta caaaatgtta taactgtagg taaactaatt ccattaatac
    16801 tatttatagg aataggtata ttttttataa aaggagttaa ttttacttct tcaacaatgg
    16861 tacctccagg agctggagca acagagtttg gagcagcagc acttttaatg ttttattcat
    16921 ttacaggatt tgaatctata gcagttgcag cagaagatat ggagaatcca caaaaaaata
    16981 taccaatagc aattataagt gtaatagtaa ttgcatcaat aatatatata ttaaatcaag
    17041 ttgtatgtgt tggtatacta ggagattcgt tatctagtac atctacacca gtggcagatg
    17101 ctgcaagaat atgctttgga aatatgggag ctggattggt tacctttgga accttggttt
    17161 ctgttggtgg aatttgtatg tgtggtgctt ttgtaaatcc aagaagttgt gtagctttag
    17221 ctgatgataa aatgttaccg agaatatttg caagaaaaga taaaaaaggt acaccatatg
    17281 tagcgataat agctactatg ctaatcacta taccaatagc tctatctgga agttttgctg
    17341 aattagcagc aataagtgca gttgctagat tcatacaata cataccaaca tctttatccg
    17401 tattagtgtt tagaaagaaa agaccagaat tagtaggtac ttttaaaact ccttttggag
    17461 cagtgattcc tctaattgct gtatgtgttg gaacatggtt attattccaa gcatctatgc
    17521 atcaattaat tatgggatta ggagctttgg caattggagt accattatac tttataatga
    17581 agtcttataa tagaaaagtc tatggtgaaa atttaaagaa aatagtttaa ttatgttata
    17641 acagtttatt gagttaaagt tagacattgg cagaaaatta aatacaaata agtatctata
    17701 aggctatatt aaaatattat taatttttta ttttaatata gccttaaatt gttatcaata
    17761 agtgaaaaat attgtaacaa tagaagaaca atagtattat gtttatagac aaaaaactta
    17821 ttaggaggta aatttatgcc aacaataaca ttaaaaaatg gtgtagacgt acttattaga
    17881 gaaggtgtaa gagaagatgc tcaaagcatt atagattttt ataatgaagt gggtggagaa
    17941 actcattttc tgtcatttgg aaaggatgaa tataaaatat ctttggagga acaagaaaat
    18001 gctatagaat cagcaaaagc atctgataat tctgttaaac tgattgcatt tatagatggt
    18061 gaaatagttg ggattgcaac gattgattct aatcaaaaag caaaaggaaa gcatgttgga
    18121 gtattaggaa ttgttgtaaa agaaaaatat tggggaattg gattgggtaa aaggcttatg
    18181 ttagacctta tagaatggtg taaatcaaat ggaataacca agaagataac tcttgtcact
    18241 aatgaagaaa attataatgc tataggatta tacaaaaaag ttggatttga agtagaaagc
    18301 atactaaaga aagaatgtta ttataatggt gtttatactg atttaatagg aatgtcactt
    18361 ttattgggaa tataattagt gtacatttta tctcgaaaaa agtatattaa gtatggtata
    18421 attaaaaaag aatataactt aatataattt tggaggacga attatgaaaa atctgtgtaa
    18481 aatatcagat gattatttaa acagcaagct taagtattta aagttattat caaaacaata
    18541 tccaagtata tcaaaagcaa gtacagaaat aataaattta gaggctatat taaatcttcc
    18601 aaagggaaca gagcatttta taacagatgt acatggtgaa tatgaaccat ttgtacatgt
    18661 attaaaaaat ggttctggtg taataaagag aaaaatagaa gaattgtttt caaatacaat
    18721 aagagacagc gaaaaaaaaa tgttagcaac tcttgtatat tatcctgaac aaaagttaga
    18781 cctaataatt aagcaagagg aaaatataga tgatttttat agaataaata tttatagact
    18841 tatagaactt tgtaaatatg cttctagcaa atatactagg tctaaagtta gaaaactgtt
    18901 accagaaaat tttaaataca taatagaaga acttttgcat gagcatgtta aaagtgaaca
    18961 taaagaagag tattataaaa gtatagttga aacaatagtt gacataggaa tagctaagga
    19021 gtttataatt gctatttcta cagttataca aaaattggtt gtagatagac ttcatgtaat
    19081 aggagatatt tatgatagag gtccaagacc agatataatt gtcgataagc ttattgaaca
    19141 tcattgtgta gatattcaat ggggaaatca tgatatattg tggatgggtg cagcctcagg
    19201 tgaaaaaact tgtatagcaa atgcactgag gatatcagct agatatgcga atttagacat
    19261 tgtagaagac atatatggaa taaatctatt gcctttagcc acttttgcta tagaaatgta
    19321 taaagatgac ccttgtaaag aatttattcc aaaggttaat gaccaaagtg taacaacaac
    19381 agaaaagtct ttaatggcaa aaatgcacaa agcaataagt ataattcaat ttaagcttga
    19441 aggtgaggtt ataagacgaa gacctgaatt tgaaatggaa catagactct tgcttaatat
    19501 gataaactat gatgaaggta cgattaattt aaaaggtaaa acatataaat taaaagatac
    19561 ttatttacct acaatagaca aaaaagaccc atataaatta actatggaag aaagaaatgt
    19621 tattgataaa ttagtatctt catttagagg tagtgaaaaa ttacaaaaac atgtttcatt
    19681 tttgttttct aaaggaagta tatatttaaa ggctaactca aatttactga ttcatggatg
    19741 tgtaccgtta aatgaagatg gaagctttat gtctatgaat ataatgggaa aagagtataa
    19801 aggtaaggct cttatggata agatggagtc tctagcaaga gaaggatttt tctttaaaga
    19861 taaggcagaa gaaaaactgt atggcatgga tattatgtgg tatttatgga ctgggaaatg
    19921 ttcatcatta tttggtaaag atgatatgac tacatttgaa agatacttta tagctgaaaa
    19981 agagactcat aaagaaaata agaacccata ttttaaactt agagaaaatg agatggcttg
    20041 taaaagatta tttgaagaat ttgatttaga acttgatgaa tctcatataa taaatggtca
    20101 tgttcctgtg gagagtaaaa atggagagag cccaataaaa gctaatggta aaattctcgt
    20161 tatagatggt ggtttttcta gggcttacca aaaaactact ggtatagctg gatacacttt
    20221 gatatacaat tctagaactt tgcaattagt atcgcatgaa ccatttaatt ctgctgaaga
    20281 agccattgca aatgaaagtg atatcttatc gacaactgtt gtagtagagc ataaggcaaa
    20341 gagaaaaatg gtgagagata ctgatgaagg aataaagata caagaggaaa tagaagattt
    20401 aaaactactt ttaatggctt ataagaaagg gctaataaaa gaaatgtaga tgttatagag
    20461 gcacttcatg attgaagtgt ctttttgtta atacttatta aatatattat aaagattaat
    20521 aatagtagtt tatattatac ttactaagtg aatccaaaat aaatgggttc acttttttta
    20581 tttataaaca ttattaaaaa aatatagatg ttttggttat attccttgtc ctttataaat
    20641 agaattatat aaacaaagga ggaggacaag agatgaataa actttctgat gaaataataa
    20701 actctctttc tacaactata agagaaaagg tagaaaaagt ctcaaacaat aatctgaaca
    20761 tagaagaaat taggttacgc tcacaaaaac cattgatact aaatgcaaac tctaaagatt
    20821 atttttacaa tcaaaaaact atgacacttg atttaaatca acaaaactca tatgtagtaa
    20881 caagagaaga tgtagagcaa acttttcaaa taatatgtaa atattcaata cattcattta
    20941 tggatgacat aaaaaaagga ttcataactt tgagaggagg tcatagagta gggttagttg
    21001 gaaaagcaat agtagaagat ggacaagtca aaaatataaa gcatatatca tctttaaata
    21061 taagggtatc aagggaaatt attggatgct cggataagat tttaagtcat atcataaagg
    21121 gaaaaaatca gattaataat actttgataa tatcaccacc tcaatgtgga aaaacaactt
    21181 taataagaga tatagtaaga aatttaagta atggtaatga agattatgga tttaaaggtt
    21241 taaaagtggc tttagtagat gagcgtaatg agatagcagg agcttatcta ggtgttccac
    21301 aaatggatgt gggaataagg actgatataa tagaaacttg cccaaaagac ttgggtatta
    21361 caatgctttt aaggtctatg tcaccaaatg ttattgtgac agatgaaatt ggaagtgaaa
    21421 aagaaataaa ggcattatac acagcattaa atggaggtat aggattaata actactgtgc
    21481 atggagattc aatagaggat atacaaaata gaaaagaatt aaatagactt ctggataaag
    21541 agttatttaa aaaggttata attctttctg caaaaagagg agcaggaaca attgaaaaaa
    21601 tttatgattt agaagaaaag agatggtatt ttgcaaatta aaataattat tatagctttc
    21661 ttgataggaa gtagttattt gataggtgaa caaatataca aaacttatac tcgtagacat
    21721 aaacaattaa acgatttaat aagagtttta gaaattttga ggatggattt atcttttgga
    21781 ctttatacat tagaagaaat ctttaatagg ataggtggaa ataaagagtt ttgtttttgg
    21841 aaatttttct atcaaatatc agaaggactt cacaatgaac aaagtaaaac tttggaaata
    21901 ataatatcag aaaatattga tgtcttaagt aaagaaactt atctaggcaa taaagaaatt
    21961 gaggaactga aaaatctaat acttacttta gggaaaagcg atatagagtc acaacaaaga
    22021 atgatagatt tatctataga aaatttgaaa aaacaaacct atgagacaaa ggaagatata
    22081 aacaaaaaag gcgtattgta taaaaaatta gtgacattca taggtatagg tatatgtatt
    22141 atattgattt aatatgggga ggtttattat ggaaatatca ttgatattaa aggttgcagg
    22201 tgtcggtatt ctaatatcag tattgaatat gatattggaa aaaacagata gaaaagattg
    22261 ggcaggtctt actacattag caggtgtaat aatcgtattg ggtatggtaa taacagaaat
    22321 aagtgacttg tttaatactg taagaacaat gtttcaactt tactaaagag gagggagtaa
    22381 tttggaaata atgcaattaa taggaattgc aatcatttca actacactct gtttagtaat
    22441 taaaaaagat agaccagaga tagcaaattt catagctata ataactggag taataatact
    22501 attgtcagtt atgtttaagc taaattttat agtagatagt atacaagatt tggctaacaa
    22561 ggctaacatc ccgactatgt atatatcatt aataataaaa ttaattggga tagcatactt
    22621 aatggaattt gcgatacaac tttgtaaaga ttgtggggaa ggaaacattg cttcaaaact
    22681 agaatttgga ggtaaaataa tagttatgtc aatgtctttc cctatacttt tgtccattgt
    22741 agaaatggtg gtgaatataa ttccatagat gtgtatcttg gaatgaatat aagtatatta
    22801 gagagctttc gttgtattga ggtttataga gtgaagagag gcatggttgc ttatgaaaaa
    22861 aagattttta ccaatgatta taggtttttt atttactttc ttttttgtaa atatctttgc
    22921 tattatgata tttgctaatg aagtaccaag tagtgaagat aaagaatatg gtgaaacaaa
    22981 aaatagcata gataagtata tagatggtca attagacaag ctagatataa atgagattca
    23041 agactatata aataaagaaa tagttattaa tgatgttaat ttaaaatcat ttgtaaaaga
    23101 tttaattagt ggagaaaaaa atatcttaga tttattcaat aaagatggat tgaagatatt
    23161 gatgtttgat gaatttaaag caagtttaaa ggtagtagca gtaattttgg tactggctct
    23221 gttatcatct attttaaaga gtctggaaaa ctctttttca tcaggtgcag taagtcaaat
    23281 agcaacatat ataatattta tcacaatggt atcacttaca cttattggtt ttaaagatgt
    23341 attacagatt tgctatgatg caatagacca tactgtaggt cttatgcaag ttataatgcc
    23401 aatattaata acatttttac ttttaattgg attcccaata acgtcgacta ctttgaatcc
    23461 tatcttcata ggaggagtaa catttataaa tgtatttttt aaaaattttc tatttgtatc
    23521 tattactgtt gcatttggaa ttttaataat aaacaattta tcaaaaaata taaggctaaa
    23581 gagatttttc tcctttgtaa aacaaataaa ttatgtttct attggagcta tgtttactgt
    23641 ttacttaggt cttgtatcca tacaaggact gtatgtaacc agttttgata aattcagtgt
    23701 aaagactgct aaatttgcta taggtaattt tattcctgtt gtaggtggat ttgtatctga
    23761 ctctgtggat attttattat cttcatctca gcttataaaa aatatatttg gaggaatagg
    23821 gcttatctta cttgttggaa tttgcttact tccagtaata aaaattttat cagtaatagt
    23881 agtgtacaaa ttagcagcaa taattgtaga gcctgttgga gaggatggaa tatctaattt
    23941 tttaaatgaa gtggcaaatt taatgataat aatgctagca tcagtaattg ctataacagt
    24001 aatgttcttt gtaactgtag ctatcttgac ctcgataagt gtcgtaagtc aagggtaatt
    24061 tatatatcag tgaaaaataa ttacatttaa tctaatgttt ttagagagga ggatgctatg
    24121 ttagagggca taaaggcatg gatagttagt gttttgatag gagcatttat agtaaatata
    24181 gtagatatga ttttaccaag ttctaagata aaaccttatg tcaacctagt tttaaacttt
    24241 atgtttgtat ttattgtaat aactccagta gtaggatttt tttcaaaaga tatgagttta
    24301 gaagatagaa tattaaaatc tatgggaaac tacaacaagc agtatgtaga tagtacaaat
    24361 gctttggcaa aagagactgg aaataatagt ctatccaagg gatatgagga tgggttaaaa
    24421 gaagtactta aattaaaact tgatgaatat ggttatgatt tagaagatat agagcttaat
    24481 ggggcaaata taaataatat aaaaataaaa gaaaagaata atagtgcaaa aagtaataac
    24541 agttccaatg aagaaaataa aaataatagt actaaaagag aggaagaaaa cataaactct
    24601 aatgacaaag aaaattctaa acaagtcttc aaaaaaggta cagagtatgg gttgaaccta
    24661 aatgaagaaa aattaaaaaa cgacttaata aaggtgctgg atgtttctat agaagatata
    24721 caaattgata aataggagtt gggagaatgt tgaaaaactt aaatgacaaa gataagagaa
    24781 aggtatattc actaataact atagcaggaa tatgtgtggt ttcattagta ttattatctt
    24841 gttttcctag tagcaaaagc gataaacaag ttggaaaaac agagactaat aaaaatgctg
    24901 aaaagcaagt cactaaagaa caagaaaaag atgatttaga gtcaaaatta acagcaatac
    24961 tatctaaaat tgatggtgca ggagacgttg atgtaatggt tacatttgaa tcaagtgaag
    25021 aaatacaacc tgcttttaat tcaaataata caacagaaac aacagaggaa aaagatgcac
    25081 aaggtggaga aagaacagtt acgacttcta gtgaaaataa aacaatgata acttcaaatt
    25141 caagtgaccc tgtagttata aaaactacag agccaaagat aaagggagtg atagtggtag
    25201 caagtggagc aagtgaccca agtgttaaag agacacttta tagtgcagta caaacatcat
    25261 tacaagtagc tggtcatcaa gtagaaatat attctaaata aatacatata accgggggga
    25321 taaatgaatg aagtttaatt ataagggaag aggatttgta ataataactt taactgcaat
    25381 gttagtggta gtaggaacag tgaattatca attaagtaag aaatctttat tagaaacatc
    25441 aaaagagttt aaggcatatg aagaagcaca gttgcaaaaa aatactgatg atagtgatag
    25501 tagtaagaca gaggattcta gtaatgtgga taaacaaggt ggtaaggaaa gtgctgatat
    25561 tgatatagtc gatagcaaag ctagtaaagt aaaagaaaag gcaactgaga caagtaagga
    25621 aataaaagct caattatcat cagagaaaaa tatgaaaaag gcatcttata ttttagatat
    25681 gaaaatgaat agagaaaaac aaagaaatga gttagttcaa gatttaaatg aaatgataaa
    25741 taatccatct acaacagaag aatctagaaa agaagcttct aatatgaaat taaatatagt
    25801 taaaattcaa gaaaaagaac ttcaaataga aaaccttttg agtacaaaag ggtatgaaga
    25861 agcgttagtt tatataagcg ataacaaagt taatgtagtt gtaaatgaag ctaaattaga
    25921 aaagaaagat gcagcaaaaa tatttgattt agttgcagaa caagccaatg tgaagtatga
    25981 aaatattaaa cttacaaata ataatagtaa taagtaaagt ataaacctca gaattaactg
    26041 aggttatttt cttgcaaagt agacttgtat ttggtattat tatacttata aaaatagttc
    26101 cttaaaatca ggaggtaagt tttaatatgg aagataataa atttggacaa gtaaaaatat
    26161 ctaatgatgt aatagctaca atagctggtt tagcagcatt agaagttgaa ggcatagaaa
    26221 ctacagcaac attaacagat aagttgttaa aaaataatgg agtaaaaata caaatcgaag
    26281 aagaggatgt taatttagat gtaatggtta cgataaagta tggaatgtca ataccagata
    26341 cagcttttaa agttcaagaa aatgtaaaaa atactgttga gacaatgaca ggactgaagg
    26401 tttctcaagt aaatatacac attcaaggaa tcagctttaa gaaggataaa gttgataaag
    26461 aagaagcaaa agcaactaag aaaaattaat attagccctc tgaaaagtca gagggttttt
    26521 tgctggtaat tgattttata tggataatta aacttaaagg agaattttga ttgatgaaaa
    26581 aagatagggc acaaaagagt acaacaagag aatatataat gaaacttata taccaaataa
    26641 atattaataa agaagatttt gaaactttag aagataaagt agataatttt ttaaaagata
    26701 attcagaaca tattataaat agatataaag aacttgcact tcaatactca aaaaatacaa
    26761 atttaaagtt agaagatact gaaattgaag atgttataga caaaaaatat ataaatacag
    26821 tatgcaaagc attgaaagaa aatcatgaca aaatagatga gttaataaat aagcatgcta
    26881 aaaactggac agtagataga atgccaaagg tagatgtttc tatacttaga ttgtctgtgt
    26941 gtgaaatact ttatttagac actcctaata aagtatcaat aaatgaagca gtagaacttg
    27001 caaaaatata ttgtgatgat aagtctccta aatttataaa tggtatattg ggaagtgttg
    27061 ttgatgaaat tgggaaataa tataataatt ggaattgata ctagctgcta tactacttct
    27121 atagcagcta tctctttaga taagaaagtt atattcaatg aaaaaataat gttagaagta
    27181 agggataatt caaaaggttt aaggcaaagt gaagctgtat ttcaacatat caataattta
    27241 gggattctta gtgataggat aaaatctttt aaagataagt ttaatgtaga aggagtttgc
    27301 tcatcaaaaa aaccgagacc tgttgaaaac tcatatatgc ctgtattcaa tgtaggtcat
    27361 aattttggaa aattattgtc tagtatatat ggttgtaggt tttatgagac tactcatcaa
    27421 gagaatcata ttgaagcaag tttattaaat agtaaattaa aaaataataa taaatttatt
    27481 tctgttcata tgtctggtgg tactacagag atacttttaa caagcaaaca agatagtcat
    27541 cacaatgtat gtgatactaa tttaggtaaa attgccaaaa taagtattaa aaaagatgat
    27601 aaaagtaagt tatacaataa ttttggatat aatatagata taattggagg aagtaaagat
    27661 attagctttg gtcaacttat agatagagta ggaattaagc ttggatataa atttccttca
    27721 ggaaagtatt tggatgaaaa tgctttaaat tgtaatctta aaatagaaag tggtctgaaa
    27781 acttctgtaa gagatgggta tatgaattta tcaggattag aaaatcaggt aaataagatt
    27841 ataaatgata atggagataa tactaatcaa aaagaatata tatctaaatt agtattagat
    27901 tcagtagtta gaaatatgtt taaatctcta gtgtatttat gtgaaactta taatgtaaat
    27961 gaagtaatat ttgctggtgg agtttctgca agtaagtata ttcttagaga attaagcatg
    28021 aagttaagaa aaaagcatat agaagcttat tttacagaac cacaatattc aactgataat
    28081 gctgtcgggt gtgctataat aggattgaat aactttttag gagaaagagt atgaaactaa
    28141 gagctttaga tataagtgaa gcaaactcat atattaaaag aattttaata aacgacccaa
    28201 tactttctaa tcttaaagtt aaaggagaaa tttctaattt taaggttcat agtagtggga
    28261 atgtatattt atcacttaaa gatgaaacat caaagttaaa ctgtgtaatt tttaaaagta
    28321 attttaatcg caatttaaaa ttagataatg gtgtaaaaat tatagctaat ggatatatat
    28381 cagtctatga aagagatggt gcgtatcaat tatacattaa tgaaattgaa atagagggaa
    28441 taggaaattt acacatagag tttaatagat taaaggaaaa acttaataaa gaaggattat
    28501 ttgaccctaa atacaagata cctataccta aaatgccaaa ctcaattggc gttataacat
    28561 ctccaacagg tgctgttata agagatataa taaatgtaat aaaaagaaga tatccgaaag
    28621 ttaatataaa gctttatcca gtaacggttc aaggagataa atcagctgaa gaaatctgtg
    28681 aagcaattag atttttcaat catatgaaga atgttgatac acttatagtt ggtagaggtg
    28741 ggggttcaat agaagaactt tggtctttta atgatgaaat ggtagcaaga gaggttttta
    28801 attctcaaat tccgataata tcagcagttg ggcatgaaac ggattttact atttgtgatt
    28861 ttgtgtctga tatgagagcc ccaacgccat cagcagcagc tgagattgct actccatcat
    28921 tggatgatat aaactataaa cttggaaaca taaaaagtag gatgagtaag tctcttacaa
    28981 atcaaataga gctagaccaa tataggttag aaacagtatt taataagata aataactatt
    29041 tagattcgta cacaataaaa gataaagtta tacaattaga caaaatatat gataaaataa
    29101 tttttggaat agaaaataat ttaaaattag aagatgaaaa attagtaaaa attggagcat
    29161 tactccataa tttaagtcca ttagctacta tggatagagg ttatagtata actcaaaaaa
    29221 atggaaaagt aataaatagt ataaaaggat taaaaataaa ggatagtata gatatagtat
    29281 taaaagatgg aaatttagaa tgtatgatag ataaaattga aaataaagag ggatagtatg
    29341 aatttaacct acgaagaagc ttataaaaga ttagaaagta ttttaagtga attagaatct
    29401 aaaaatgcta gcttggatga atctttaagc ctatatgaag aaggtataag cctttataaa
    29461 cattgtaata agcttttaga tgatgctaaa ttgaaaataa gtaaatttaa tcaattggga
    29521 atagaagaag actttaaaat agaggaggaa taaaattgga atttaaacaa tgtctaaaag
    29581 aaaaagcaag ttttgtagaa aaagtactta aagaatatat gcctaaagaa gaagggtatc
    29641 aaaaaactgt tattgaagct atgaactata gcttaagtgc tggaggtaaa agattaagac
    29701 caatacttac attggaggct tgtaaaatag ttggaggtaa tgaagatgaa gctattcctt
    29761 ttgctattgc aattgaaatg atacatacat attcactaat acatgatgat ttgccagctc
    29821 ttgacaatga tgatttaaga agaggaagac caacaaatca taaagtatat ggagaagcta
    29881 tggggatttt agcaggagat gctttgttaa actatgcttt tgaagtaatg cttgcaggct
    29941 caatcaataa agaaaatcca gaaaagtatc taaaagctat aaatgaaatt gccaaaggtg
    30001 ctggtatata tggaatgata ggtggtcaag tagtagatgt agaaagcgaa aacaaacaaa
    30061 ttgaaaaaga aaaattagat tatatacata tgaataaaac tgctgcaatg atggtaggct
    30121 gtatgagagc aggagctact ataggtggag caaattctga acaaatggaa gaaataacta
    30181 aatatgcaaa aaatatcgga ttatcattcc agatagtaga tgatatacta gacatagtag
    30241 gagatgaagc taagttaggt aaaaaggttg gaagtgatat agaaaatcat aagtcaacat
    30301 acccatctct tttaggttta gataaatcta aagaaattgc acataatttg attgatgaag
    30361 caaagaaaag tatagaaaaa ttatctgatg atgtagattt tttaaaggga ttggctgaat
    30421 atataataga tagagaatac taaaatatac agctaaaatc gaggagggaa aaatggattt
    30481 tttttcggag atttttaaca atggggcttt gggaataagt ctgattgctt gtttcttagc
    30541 acaatttata aaaatattta ctggtaagga aaaaagaatt gagttatcaa gaatacttat
    30601 ttcaggaggt atgcctagtt cccatagttc ttttgttaca agtttagcta cagtggtagg
    30661 tattgaaaaa gggtttaatt ctactgattt tgctataatt accgtgcttg ccttaattat
    30721 aatgtatgat gcagcaggtg ttagacgggc tgttggtaaa caagctacta tattaaatca
    30781 aatggtggca gatatacagc atggaaagca tatagagcag aagaaattaa aagaattaat
    30841 aggtcataca ccactagaag tttggtttgg agctttactt ggaatagtaa cagctttaat
    30901 attgatgtaa attgaaaatt tacagaataa aggtgaggat aatatgtata aatatttaga
    30961 taaagtaaat tctccaaagg atataaaaaa tatgagtata gaagaaatgg atttacttgc
    31021 aaaggatata agaaagttct tagtaaaatc tgtttcaaag acaggaggac acttagcttc
    31081 taatctagga gtggttgagt taacattagc acttcataaa gtatttgata gccctaaaga
    31141 taagattgta tgggatgtag ggcatcaatc ttatgttcat aaaatagtta ctggaagaaa
    31201 agattgtttt gtatctttga gacagtttaa tggactaagt ggttttccaa aggagaatga
    31261 aagtcctcat gatatttttg atacaggaca tagcagtaca tctatttcta tagctacagg
    31321 tattgcctgt gctagggata taaaaaaaga aaactatagt gtgatatctg tgataggaga
    31381 tggttctata acaggaggta tggcacttga agctttaaat caattaggat atatagatac
    31441 caatatgata gtaatcctta atgataatga gatgtccata gataaaaatg ttggaggaat
    31501 gtctaaatat ctgtcaagta ttataagaaa ctcaacagtt gagaaaatga cagatgaagt
    31561 tgataaaatt ttaaatgtta cacaaacagg tgaaatttta tctaagactg cacatagatt
    31621 taaagataaa ctaatgtata gcttttctcc tcaggactgt tcattttttg attcattagg
    31681 tataagatat tatggaccta tagatggcca taatactaaa gaactaatag atatattgag
    31741 aaaagctaaa cacaaaaaag ggcctgttct tttacatgta attacgaaaa aaggaaaagg
    31801 gtacagattt gcagaagaac aacccgataa atatcatgga gtatcaaagt ttgatataaa
    31861 gacaggtgtt acatctgcaa aagttaagtc tatgtcaatt agtgttggag aaaaacttgt
    31921 tgatatggct aatagtaatg aaaatatagt agcaataaca gctgcaatgc catctggtac
    31981 agggctaaac ctgtttgaaa gtgcatatcc aaaaagatat tatgatgttg gtatagctga
    32041 acaacatgca acaggatttg cagcaggtct tgcaaaaaat ggtatgaaac cttactttgc
    32101 tgtgtactca tcatttttgc aaagggcata tgaccaagtt attcatgatg tatgcataac
    32161 aaaaaagcca gttacttttc ttatagatag agctggatta gttggaaatg atggagaaac
    32221 tcatcatggt atgtttgatt taagttactt aaattctatt ccaaatattg tagtaatggc
    32281 tccaaaagac acaagagaga tggagcttat gatggatttg tcattaaaat tagattgtcc
    32341 actagcaatt agatacccaa gaggaagtag ttattaccta gataagggag agtatggaga
    32401 gattgtactg ggtaaatatg aggtattaga tgacggtcaa gatacagtca tactttgtat
    32461 tggaagtatg gtaaaacatg ctttagaggc taaagaaata ttatcaagag aaggaataaa
    32521 tccgacaata gttaatgcta gattcttaaa gccaattgat gaaggtatgt taaaagcatt
    32581 attaaaaaat cataaaaatg tagttactat agaggataat attgtaactg gaggatttgg
    32641 aagtagaata aataagttta ttatagataa tgaatataac gtaaatatac ttaatatagc
    32701 aataccagaa gaatttgtaa aacatggcaa tatagatgaa ttatatgatt ttgtaggatt
    32761 atcgccaaaa agtattgctg ataaaataag gaaattagtt attgagtaaa aatctaatgt
    32821 agatattgta aattaaaatt tatataaaat atagaattta tcaattagaa taaatatcaa
    32881 gtgtgttact tcaagctact ttagttatca ttaaagtagc ttttaacaga aaggtaattg
    32941 tatgaaaaaa agaatagatt tattattagt agaacaaggg tactttgaaa gtagagaaag
    33001 agccaaaaaa gcaataatgg ctggattggt ttttgttgat aatcaaaggt gtgataaggc
    33061 tggtacagaa gtaaaagaag attgtagtat agaagtaaaa ggaaatccaa taccatatgt
    33121 cagtagaggt ggtttaaagc ttgaaaaagc aatgaaaaac tttgatttga ctattgatgg
    33181 aaaggtatgt atggatatag gggcatccac aggtggtttt acagattgta tgctcaaaaa
    33241 tggagcaata aaagtatttt ctatagatgt tggttatggt caacttgcat ggaaattgag
    33301 acaagatgat agggttgtgt gtatggaaag aaccaatata agaaatgtga caatagaaga
    33361 tacaaagcag tttgctgatt ttgcgtcaat tgatgtgtct tttatatcgt taaagttagt
    33421 acttccaaag gcaaaggagt tagtacgtca tgatggggaa gttgtcgctt taattaaacc
    33481 tcaatttgaa gcaggaagag aaaaagtagg taaaaaagga gtagttagag aaaaatctac
    33541 acatatagaa gttataaaaa tgatttctga tttctcagtc gaaaatggat ttgagatttt
    33601 agggttagat ttttctccta ttaaaggtcc tgaaggtaat atagaatatc taattcattt
    33661 gagaaatgga aatgaaggat atgaatttga tggtgagaca tacaataaca aaatagtaga
    33721 agttgttgaa gcttctcata atttagacaa ataggttaaa agggggctta gatttgatcc
    33781 tcgaattata tatgaaaaat tgtgccttag ttgaagaact aaggttgaat attgataaaa
    33841 acttaaatat acttacaggt gaaactggtt caggaaaatc tataataata gatgcacttg
    33901 gactttgttt aggtgataaa tatgataggt cttttttaag aaaaggaaca gataagggac
    33961 ttgttgaggc tgtatttttt tcagacaata gatatcttaa aaaaatatta gaagaaaatg
    34021 atataagtat ggaagatgat aatttattgg taataactag gctgatatac tcagatggta
    34081 agagtactgc tagagtaaat ggaaggactg ttaaagtgtc tcttcttaaa gaaattgcat
    34141 ctacacttat agatatacat ggtcaacatc aaaatcaagc tttgtttaat aaagatactc
    34201 atttaaaatt tctagacctt tttggagaaa atgaattgga agagtttaaa attgcttata
    34261 aaaaggtgta ccataagtat agtgaagtaa aaaaagcttt aaattgcttg acagaaaata
    34321 aagatgagat gcaaattcaa cgagaaatag atttattaag atttcaaata aatgaaattg
    34381 aagctgcaaa tctaaataag aatgaatatg aagatttatt aaaacaaaga gaagtttata
    34441 gaaatagtga aaagatatat aataatctaa attcgagtta tagcaagtta cataatggtg
    34501 agtataatgt tattgaccta ataggtcttg catctaagga acttaatgac atatctaaat
    34561 atgacagtgt tttaagtgaa tatagtgata ctgtagaaag aatcatgtat gagttacaag
    34621 acatatctgg tgagataaga aattacaaag ataatataga ctttgaacct tatgagctag
    34681 agcagattga gcttagaatc gacgagatta ataacttaag aagaaaatat ggagacagta
    34741 tagatgatat atttgagtat tatggaaaaa ttaaagatag attagatgaa atattaaata
    34801 gagatgaaag agttgaacag ttaagaagca aactgatgaa tatagaagaa gatttaaaaa
    34861 taaaagccag taaattgact aaagcaagaa tagaagtagc aactacttta gaaaaaatac
    34921 ttcttgatga attaaaaagc ttaaatatga aaaatgtaat gttcaaggtt aattttgaaa
    34981 aaggtccatt tactttagat ggagtagatg atattgaatt tatgatatct tttaacttag
    35041 gagaagatat taagcctata tataaagttg catcaggtgg tgagatgtcc agatttatgt
    35101 tagcgtttaa aaccatattg gctgatatag atgatattga tacattagtg tttgacgaga
    35161 tagatactgg aataagtggg atagcagcac agatagttgg tgagaaattg agtgatatag
    35221 caaagaaaaa acagataata tgtataactc atttacctca aattgctgca aatgcagaca
    35281 ctcattattg tatagagaaa gatacttcaa ataataggac gtttaccaat gttagtaagt
    35341 taaatgaatc tcaacgaaaa aatgaaattg ccagacttat agcaggtaac aatataacag
    35401 aaaaaactat tgaacatgca agtgaaatta ttgagttggc taaaaaatgt tagtgaaaaa
    35461 caagcatttt aataggtgta aagaattttt atagaaatgc tttacatcta ttttttatga
    35521 tgaaattatt ttaaaatatc tagtgcctat attgaagaaa agtagattta aaaatagaag
    35581 aaaatttgta taaatttgta actttgaaat ttctaaatgc ttaagtataa tattatttat
    35641 taaggtagta gtgaggggga cataaaatga ataaaaaaga tactatgcac ttaattttta
    35701 caattgtaat ttttatttta ttatggtata ttgtggtagc agtaagtgag attaataaaa
    35761 atattgaaag ttgtagtaac aaattagatt atttgtcaca agagattatg agtactaaat
    35821 cggatattag taatactata aatgaagaaa tgtcaaaaag ttatataaca aagagtattg
    35881 acttaaaagt aaaaaaaatc gagaaaaaag agtgtgttat tgatgtagat gttcaattaa
    35941 gtaaacttgg aaaaaatgga aaagtatttt ttatgtataa agaagatagt gataaatgga
    36001 atgaaactga gatgacaaaa catggagaat tatcgtatgc atgtgaaatt aagataaata
    36061 caggaagtga atatgactat aaagtagtta caagtggagc tatatctgaa agttctgatg
    36121 tgcaaaaatt gactaaatct gattatatgc ctgaacaacc catatttaat tctgggataa
    36181 gagataatag agaatacttt attgaaatag tagaaaatag taatcttttc aatcatgaga
    36241 gtgaaaagtc aaaaaataaa gtttatgata atattagatt agaaaaagta gatattattg
    36301 taaatgaagg aaaaaaagac actatataca aagccaaatt agctgaaggc ttagatgatg
    36361 gcaaaactaa taattcaaat agaaatcaag tgaattatga agtaagtttt cctaaaaggg
    36421 atattgatga cataatttat attaaagcta aacttacata caataatgga gtagaatata
    36481 caaaggatat aactgaagat ataactatgg gattacaaga tttagaaaaa taaatacata
    36541 tttaaataaa tattcaaata gatttgttca ataaaataga gtataaataa aactgtagta
    36601 gttttactta tactctatta aaaaattata attttaattc catatatcta gccccttcaa
    36661 ttggattata agtataaggt tctatatcat aaaaaccaaa cttttcatat aatccttgtg
    36721 cacttttcat gcttggaaga gtatctaatc tcatgtgtgt atatccaaat tttttagctt
    36781 cttcaatgat ttcttccaat agtatttttc ctatttttaa tcctctaaat tgatttctta
    36841 catataatct ttttaattca catattttac cttcaagttt ttttaaagca acacaacctg
    36901 ccaaattttc atcaacaaat gccaatatta atgagcctga aggtttttta tattttcctg
    36961 gaagtgtttt taattcatta ttaaagtctt gaaaacataa gtctatattt agagaatttg
    37021 aatattcagt aaatagaagt tttacgtttt caaggttttc taaatctttt tcttcaacaa
    37081 attgtaaagt tatattttga gccattttat cagccattat atacacatgt ttatataatg
    37141 acttcacctc cttgatatat gtatattttt cttaatatca attagatttt aataccatta
    37201 ctttttcacc accatagtca agctctccat taaactcaaa tccaaaatct ttataaagct
    37261 taatagccat tttattgtct tcgaatatac taagatatag ttcatcacag ttatattcat
    37321 ttttaagatg atttacaaga aaatctaatg atgcttttcc atatccttta ccttgatatt
    37381 ttgaatcaat cataaatcta tcaagccaaa ttctatcatc ttctaaacga ccatacatag
    37441 caaatcctat taaagaattt tcatcatata ttccaacagg tacccaagct gttataaatt
    37501 tagattcagc aatagaaagt gcattgcttt ctatataatc taactgactt ttatctacag
    37561 acaaactaac taaatcatgc caattattta tgtctgcaat tcttacattt aatttcaaga
    37621 tattaccgcc cttcaaactt tttgttacaa tctaatacta tcatattatg tattaaaaat
    37681 cacttattaa gattttaaat aaacttttta aatgaataaa ttctcaggaa aaaggaatta
    37741 taaatacata ataatacagc ataggagtag atggatatat gaacaaaata aataaaaatt
    37801 tcaaaaatat ttggataata ttaggagctg tatcgatttt taccatggtt ataattttta
    37861 ataaaaattg taatgttaat aaagagactc aaatagaagc ttttacaatt aagaatcatg
    37921 ataaaaaatt tgtatatcct atgggaaata taattggagt aaaagcaaat acagatggtg
    37981 tattggtctt aggatatgag gaagaggatg tagactatat tggaggcata caaatcggtg
    38041 ataatatagt aaaaatagat aataaaagga taaaaaatag tcaagatgta tcagagattt
    38101 tgaataagat taaaaaaagt aaagttgaag tgacttttga gagaaaaaat aagtataaaa
    38161 cagaagtaat agagactaaa aaagaaaatg gcaagtataa acttggatta tgggtaagag
    38221 ataaaatatc tggaattgga actatgactt tttacgaccc tagtatggaa aagttcaaag
    38281 caatcggtca tgccataaaa gattcggata ctaatgaact attaaagata aagcaaggat
    38341 atatatataa gccagaacaa ttaaaaatag taaaagctag taatgaaaaa gtaggtaaaa
    38401 taaaaggaga ttttaatgac agcaatttaa tgggtaattt ctcaaataat tcagagctag
    38461 gtataagtgg taatattaca gaaaaccata ataaagagtt taatgtagca aataaagaaa
    38521 agcaattaat agaagttgga aggccgcaag atgtaaaaat aggagatgca gtaattcttt
    38581 ttgaggataa aaacaaaaat ataacaagct atgatataaa aatagaaagt atagtatatg
    38641 ataaaggaaa ttatagagat atggtaataa aagtagtaga tgacaagtta ttggaataca
    38701 caggaggtat cgtacagggg atgagtggag ctccaataat acaaaataat aaaattattg
    38761 gtgcaataac tcatgttttt agagataatc cgaaaaaagg atatggtatt tttatagatg
    38821 aaatgataaa attgtaggtg aggcattaaa aaattttatt attttatcaa ttatctagga
    38881 ggaatataat tttggagtgt cgaatatgct ttagagtaga taattaggaa gcaattgtgt
    38941 aaaaagttta gttttctgta ataagaagat gttttttaat ggggggattt ttagtggaaa
    39001 aaatcaaaat agttttagca gatgacaata aggatttttg tcaggtatta aaagagtatt
    39061 tgtctaatga agatgatatc gatatattag gcatagctaa ggatggaatt gaagcattag
    39121 acttagtaaa aaagacacaa ccggacttat taatactaga tgtaataatg ccacatctag
    39181 atggattagg tgtaattgaa aaattgaata ctatggatat acctaaaatg ccaaaaataa
    39241 tagtactatc agcagtaggt caagataaga taactcaaag cgcaataaat ctaggagcag
    39301 attactacat agtaaagcca tttgattttg ttgtgtttat aaacagaatt agagagctag
    39361 tttctaatag agttacacaa gttgagccaa aacctagacc agttcaagag actcaaatga
    39421 ctagaagtga ttttgttaag aatgtaggaa atatagagac agaaatcaca aatataatac
    39481 atgaaatagg agtaccagct catataaaag gatatttata tttaagagaa gcaataaaaa
    39541 tggttattga taatgttgag cttttaggtg cagtaacaaa agagttatat ccaagtatag
    39601 ctaaaaaatt caatacaaca ccaagtagag ttgaaagagc aataagacat gcaatagagg
    39661 ttgcatggag tagaggaaaa gttgacacaa taaatcaatt atttggatat acggtacaca
    39721 atactaaagg aaaaccaact aattcagaat ttatagcaat gattgctgat aaattaagac
    39781 tagaacatag tatggttaaa taaacaagac ataaaaagta aggcttttta attaaggcat
    39841 tggctataaa tgcgtattac aagcagcgaa acggtataac cactagggtt ataccgtttc
    39901 gctattttaa taaatataaa aattttcttt attatttgct tactatatca atataataat
    39961 tttattatac tatggatata gtatgtgtct ttacaagttg taaactgaca gtggtttatt
    40021 ttttaatata aatattgact ttgatgcagg taaactttgt atttttaagc gtattgtgga
    40081 atatgttaaa taaaaaaatg atgaaatata gtattgtaaa tgccaaagat gcaaaacaaa
    40141 cttaaaacat ttattttatt gttaagtaat gctataatat aatgtgattt taataatgat
    40201 agtggaggtt taaatatgag agtcgaggcc cctataaaag tagatcgaaa aaccaaaaaa
    40261 cttgctaaaa gagttgaaag tggggaaata gcagttataa atcatataga catagatgaa
    40321 gttgctgcaa actctttagt agaagctaaa ataaaacttg tcataaatgc ggctccttct
    40381 ataagtggta ggtatcccaa taaaggtcca gggatattaa ctgaaaataa tatactaata
    40441 attgataatg ttggagaaga gttatttgaa gaattaaaag aaggagaaac tatagaagtt
    40501 gtagatggaa agatatatag aaaaggaaaa tttttaggtg caggagaagt tttagacaag
    40561 tatgaggttt cacaccaaat aaaggctgcc tatgagaatt tagctgtaga attggataga
    40621 tttatagata atactataga ttatgctaaa aaagaaaaag gatttatact tggagaagta
    40681 gaaattccaa aagtgaaaac taattatgct aataaacatg tattgatagt tgtaagaggt
    40741 caggactaca aagaagattt aagtactatg ctttcttata tagaagaggt aaagccagta
    40801 ttggttggtg ttgatggagg agcagacgct ttaattgagt ttggatatac tccagatgtg
    40861 attgtaggtg atatggatag tgtaagtgat gaagctttaa aaaaggctag tgaaatagta
    40921 gtacatgctt atacagatgg aagagcacca ggtcttaaga gggttgagga attaggctta
    40981 gatgcagttg tatttcctgc accaggaact agtgaagata tagctatgct tatagcctat
    41041 gagtataagg ctgagttaat agtagcagtt ggtacacatt caaatatgat tgattttctt
    41101 gaaaaaggaa gaaaaggtat ggcaagtact tttttagtta gactaaaaat aggttcaaag
    41161 cttatagatg ctaaaggtgt taatttgtta tacagaagta aattaaaaat aaagtacata
    41221 tgggcattaa tagcaacagc attatttcct gtgttggtag ttgcatcatt gtcaccagga
    41281 gttcaacagt tcatacaact aatgcaatta aagtttagag tattattaca gatgtaaatt
    41341 ttaggaggat aaatatgcat attaatatga aatattatat agtcactata ggggctatat
    41401 ttatagcttt gggtataggc atgttagtag gatttaattt aaataataat caacaattaa
    41461 gtgaacaaca agcaaacata ataaatgatt tggatgataa gtttaatatc ttaaaagaaa
    41521 aaaatgataa acttgacagt gacttagcaa gtgtaaatag agattatgaa gaggcagtta
    41581 attttataaa taaaaatgta gataagattt tagctggttc gctgaatggt aaaagtatag
    41641 gaataatatc tgctaatgaa aatgacgatt atacaaaaaa tatagaagac ataataaata
    41701 aatctaatgg aagcattgca tttaatataa tattaaaaga aaacataaca aatccagaaa
    41761 agttaaagga aatgtcaact aaactaggta cagatgtaaa aaatgcaaat gatgcagtta
    41821 attatataat tgacactctt aaaaaggaag atgcaagtga cattttaaca tatcttcaag
    41881 aactagatgt aataaagttt aattttattg gagatacata tctaaaatat gattccgttg
    41941 taatagctgg tggaaatgat gcaaaagaca gtacaaaaca gtttgaaaaa atagaaaaat
    42001 ttgtagtttc aaaacttaaa tcagagaata aatatttagt agaagttcaa aatacaggag
    42061 ttaaaacttc ttatgtagaa ctatattcta aaaacaaagt tgcaactata gacaatattg
    42121 atgaaggtat aggaagtata tctctagcga ttcttctaca acaaggtaat atagttggta
    42181 attttggtag attggatact gcaacttcat tgttgccatc tattaaatag aaataatttt
    42241 aaggagagaa aaaatggatt cttatataag tataataata ccagcttata atgaagaaag
    42301 tagaataaaa tatacattag aaagcatatt ggacattaag caaataaatg aaataatagt
    42361 tgtggatgat ggttcaattg acaatactgc taaggttgta tctgagctag aaaatgaaaa
    42421 gattaaattt tttaagttag ataaaaatag aggtaaagga tatgctttaa attatggtat
    42481 caaattagca atgaaaaatg cagatattat aggttttctg gatggagatt taggaagctc
    42541 tgctaaagaa gttgaaaaat taatagtacc tatattaaat ggtgaggcgg atgttacaat
    42601 agccaagttt cctcctgcaa agagaaaagg aggtttggga tttgtaaaag gtttagctaa
    42661 aaattctgtg tttgagatga ctggtgttga gttagatgca actttgtcag gtcaaagaat
    42721 atttaaaaga gaagttttag aaaagtttga tgaaatacct tttggatatg gagtagaagt
    42781 tggaatgacc attgatatat taaagtatgg atttaaaatt aaagaagtat tagtaaatat
    42841 gactcataat gaaacaggaa gaaatttaaa aggttttatt catagaggga aacagtatta
    42901 tcacataaaa aaggttttag gacaaaaaaa gaaagaatgg aggtaatatc ttagtgaaag
    42961 attatatact ttataccatt ttattattaa ctggattatt tggaacctat gctgtaattc
    43021 cactatttaa aaatttattg ataaatggaa atgtacttag accaaactat aaaaaagata
    43081 tgataccagt aagtatggga attgtatttt tgcctatgat tgtaatcaat ggaataatac
    43141 ttgctttctt tactacagaa tttaaggatt tagcgtatat atttatgttt atgtttggta
    43201 tgatatccat gttttttgca gggatactag atgatgtcat tggaaataga gatgtaagtg
    43261 gtttaaaggg gcattttaaa agtttattta aaggaagtct tacaacagga ggttttaaag
    43321 ctttatttgg tggatttgta gggcttatag tttctgttgc tatatcaaaa gacataattg
    43381 atataatagt aaatacacta ataatagctc tatcaactaa tttaatgaat cttttggatt
    43441 taagaccagg aagggcaata aaagcatatt tattcataat ggtaattata tttttaacat
    43501 tgacaggatt tgttcaagtt cttccattac ttatagtacc aaatgtactt gcatacttta
    43561 attatgattt aaaagcaagg gctatgatgg gagatactgg ttcaaacgtc cttggaatat
    43621 ctattggaat gctcatttct tttgggtatc catttaacat tagattagga tggttaatat
    43681 ttgtagttat tatgcatata ttcactgaga aatactcatt aactaagata atagaaaaaa
    43741 ataaactatt aaattttata gataaattgg gaaggtagta ttatgataag caaaagagtg
    43801 gggattgttg aatcaatagt aagtcaaact gaaactctag atgatataag agtaaatata
    43861 aatggagaaa tacaaagggc atataattat ccaaaaatat ctggaactat aaatataggt
    43921 gacgaagtgg ttttaaatac aacagcagta gaactgagct taggtacagg tggataccat
    43981 tttgtaataa ctaatttaaa taatatagaa tctactttaa ctgaaggtgg acatataatg
    44041 aaacttagat atacgcctct tcaaataaaa gtggattctg ttgaggaaca agaaagtatt
    44101 tatcatgata aatttgcgaa ttttaaagga ttagaaggtc tttctgttgt agtagggact
    44161 cttcatagta tgttaactcc atttgcagct tcatttaaaa gaaataatcc aaataaaaaa
    44221 ttagtgtata taatgactga tggagcatct cttccaattt atttaagtaa aaatgtggat
    44281 actttaaaag aaaaaaaatt aatagatagt acaataacta taggaaatgc gtttggtgga
    44341 gattatgaat gtataaatat atatactgca ttaattacag caaaagaaat actaaaagcg
    44401 gatgcagtat ttgtcagtat gggacctgga attgctggga caggaacaaa atatggattt
    44461 acagggattg aacaaggctc tattcttgat gccgtaaaaa aacttgaagg aagagcaatt
    44521 gcaattccaa gaattagttt tgctgacaaa cgagaaagac atcaaggtat ttcacatcat
    44581 agtatgactg tatttaaaga aattgtaaat gtaaatgtag acataccaat aactatatat
    44641 gatgatgaaa aattgaactt tataaaagag caaatcaatt taaatggttt agacaaaaag
    44701 cataatataa tttatataga taataataaa acgaaagagg atttagacta ttttaatcta
    44761 aaagtaaaga gtatgggaag aaattatgaa caagatgagg catttttcaa agctgcaagc
    44821 acagcagcat attacttaat ggaggtttaa gatggtatta gaagaaaaaa ctattagtag
    44881 tgatagggtt tatacaggta aagtaattac tttaaaggtt gatactgttg aaattccagg
    44941 gcaaggatat caaaaaaggg agttagtaga agttggtgga gcagtaggaa tagttgcaat
    45001 cacagatgat aataaagttg tgttagttaa gcaatttaga aaacctatag aaaagccaat
    45061 ctttgaaatt ccagctggta aactagaaaa aaatgaaagt ccaaaagaat gtgctgaaag
    45121 agaattaaaa gaagaaactg ggtatagtgc aaaaaatatt aagctaatac ataaattttt
    45181 cacttcagca ggtttttcta atgagataat gtttgtatat ttagcaacag gtcttactcc
    45241 aggagagaat aatttagatg cagatgagtt tttagatgtc tatgaaattg agcttgaaga
    45301 agcatataac atggtattaa aaaatgacgt tgaagatgca aaaacatcta ttggattatt
    45361 attagtaaaa gatatgttta aaaattaata tttataaaat gaataaccct ttgtcctaaa
    45421 gcatactatt ttataaagga caagggggaa ttaaattgag aagaacatat agaaaaaatg
    45481 attttagaga attaaataga caaataatta ttataggatt gttgtttatg atgtctattg
    45541 ttgttgggtc ttacataaat aaaatattgc caggttctag taacaatata ttaaacaata
    45601 taaatcctgc tgtagagtat tataatctaa atataagcat aaaagataca gtgatacaaa
    45661 atttaaaatc agatgcaatc tttatgggct caatagctct attaagtcta tttgtagtaa
    45721 caattccagc agtgttggta gcttttgtat taaaagggat gtcaattggt tatacaataa
    45781 atagctgtat attggcatta aaatttaaaa gtataaaaat gattttaata atattattta
    45841 aaaatttgat tattattcca tgtgcaatca tattagcatt aattagttta agttatttta
    45901 aagaaatggt ttatgaattt aaaaagaaaa atagaaaaaa tatgcaattt ttaattaaga
    45961 ggtatatact aaatattata attataatag cactatcatt ggggttgcaa ttaatattaa
    46021 atactgcaag tattggtatt attaagttcc tagctaaata aaggagttta taatggatat
    46081 tatagaggga tatatagatt acataaaaga taaaaaaaaa ttatcagaga acacagtggc
    46141 atcatacttt atggatataa aaaaatatat ggaatatata aatcaaaaaa gcattaaact
    46201 agtagatata gtagaaaatg atattataag ttacttaata aagttggaaa aagataatgt
    46261 atcaatagct acaatagcga ggatgatatc ctctataaaa tcttttcatg attatttatt
    46321 tttaaatcat atatgtacaa ataatcctgc aaaggatata aaaaaaccta aaataaaaaa
    46381 agaaaatata aatatactta cagaagaaga aattgaaaga ctattaaatt ttccaaagtt
    46441 aacaacgcct aaattgatta gggacaaggc tatttttgaa gtattatatg gtactggtat
    46501 aaaagtatct gaattagtag agatgaatat agaggatatt gacctagata ttgattatat
    46561 atattgtaat tcatgttgta aaaatcaaag agtgatacca ctttgtgata ttacaaaact
    46621 ttacttagaa aagtatctaa aagaagcaag acctaaaatg gctgtagaag gtgaaaaatc
    46681 attattcgta agttcattag gtcaaaggtt tacacgacaa ggtctttgga aagtcataaa
    46741 aaaatattct aatctagcca atatagataa aaatataaat cctacaatgc ttagacattc
    46801 atttgctatt catttgttaa atgaaggtgc taacattgct gttgtaagta aaattttagg
    46861 aaatgttaat ttatctagtt tgcaagtata tttaaatcat atagataaaa acgtgagaag
    46921 agaaataaag gagaaacatc ctagaaatga tgttgatgta gaattacaaa aagcagaagc
    46981 taaatagact atatagcaaa ttttataatt tagaaggagc atatttatat gagcagagta
    47041 atttggattg taattgatag tgttgggata ggagcattac ctgatgctga aaaatttggt
    47101 gatagtaagg atgtaagtac ccttggaaat atatttaagg aatatccaga tattcagatt
    47161 cctaatatga gaaatttagg tataggaaac atagatggta tagatttttt tgaaagtata
    47221 aaagaaccaa taggttgttt tggaaagtgt aaggaaatgt ctcagggaaa agatactact
    47281 acaggtcatt gggaaatgac tggtataata gttgacaaac catttaagac atttgaacat
    47341 ggattctcta aggagataat agaagagttt gagaaaaaaa ctggaagaaa agtagtagga
    47401 aataaaccag cttcaggaac tgtaataata gatgagtatg gagagcatca aataaaaact
    47461 ggagatgtaa tagtgtatac atcagctgat agtgtatttc agattgctgc taatgaagaa
    47521 gtaattcccc tagaagaact ctataatatg tgcaaaatcg ctagggaaat aatgatgggg
    47581 gataatgcag tagctagagt aattgctaga ccattcatag gtaagaaaaa aggtgagttt
    47641 gtgaggactt caaatagaag agattattca ctagacccat ttgaaccaac agttcttgat
    47701 aatataaaag aatcaggact tgatgtttta gcagttggaa aaatagaaga tatttttaat
    47761 ggaaaaggaa ttacagatgc tatccacact aaaagtaata tggatggagt ggatgaaact
    47821 ttaaattata tgaaacaaga taataaaggt ttaatatatt caaatctcgt tgattttgat
    47881 tctaaatatg gtcatagaag agacccagaa ggatataaga aagctcttga agagtttgat
    47941 agtagacttc ctgaaataat ggctaatatg agagaagatg atattttaat aatcaatgca
    48001 gaccatggaa atgacccaac atataaaggt acagaccata caagagaata tatacctgtt
    48061 atgatttatg gcaataagat taaaaaaggt tttaatttag gagtaaaaga tacttttgct
    48121 gatattggag caactgttgc agacatacta aatgtaaagt tgcctaaaca tgggtcaagc
    48181 tttaaagaag atttatttta aattataaaa tgataaaaac ttagcttaat aggatggaga
    48241 gattggaatg tttgaaaaga tagcacaaag tagtaaattt ataaattcta aaagtaatat
    48301 aaaacctaag attggtttga tattaggttc tggactaggt gatttagcaa atgatataga
    48361 agaccctgtc acaattaaat atagtgaaat tccaaacttc cctgtatcaa cagtagcagg
    48421 acatgctgga caacttgtaa ttggaaaact tgaaggaaaa gaagttattg ctatgcaagg
    48481 tagatttcat tactatgaag gatattctca gaaagaagct acttttccaa taagggttat
    48541 gaaagaatta ggagtcgaga ttcttatagt aactaacgct gctggaggcg taaataaaga
    48601 atttaaagca ggagatttga tgcttataag agaccatata aactttagtg gaagtaatcc
    48661 attagtagga aaaaatgatg acagatttgg tgctagattt ccagatatgt cggatgcata
    48721 ttcttctaaa tatgtagatg tagttaaaaa ctgcgctaaa gaatgtaata tagatgtcaa
    48781 agaaggcgtt tatatgtttt ttagtggtcc aaactatgaa acaccagcag aagtgagaat
    48841 ggcacaaatt ttgggagctg atgctgtagg tatgtctact gttccagaag tcatagtagc
    48901 ttctcactct aatataggag ttattggtat atcttgtata acaaatatgg ctgctggcat
    48961 cttaaatcaa ccactaagtc atgaggaagt aatagaaact actcaaaaag ttaaatcaga
    49021 atttatgaat ttagttaaaa caacggttaa aaatttataa gttaaagctt attatagatt
    49081 ttttgaggaa gtttataata aaataggtaa tgtttaattt gaaa
//
LOCUS       DBGJFF010000011        49012 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.011, whole genome shotgun sequence.
ACCESSION   DBGJFF010000011 DBGJFF010000000
VERSION     DBGJFF010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 49012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49012
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.011"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            62..406
                     /locus_tag="ACOIAF_000735"
     CDS             62..406
                     /locus_tag="ACOIAF_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HGZ2667761.1"
                     /translation="MEIKRYEGTGRMSRAVVHNNTVYLCGQTHAEGGVIEQTTEVLAK
                     IEDLLNKYGSDKQHLLSVTIYLRDMKDFEAMNSVWDAWVEKGFEPARACVEARLAREH
                     LLVEMSVVAATK"
     gene            complement(565..2661)
                     /locus_tag="ACOIAF_000736"
     CDS             complement(565..2661)
                     /locus_tag="ACOIAF_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HGZ2667762.1"
                     /translation="MIKFKLYISGLYSGNVIFDGDLLIEKLNPFTNKIESLKPISKEE
                     NTYYLNLTKIDLKSLFNNFDVYTKSLVNTDKDTVINNLGETHSKLNEYIWVQRNKKFP
                     LDIIIVDNKIVGFICLSRETCTILIMDGYEEYTVLKEWEKTHKNEEIYSIRFGGNYMI
                     DMKDGIKLSTDVYLPDFVDSAKKAPTILMRTPYGKENDKEIYYKYVQRGYAVVIQDVR
                     GRNESEGKWEPMIHEREDGDSTINWIVSQEWSSGIVGMLGASYLGYVQWAAASSGNKH
                     LKALVSIVTSGSPFIDIPRKGGAFVSGMLAWAFMVSRNKVDRSKMVRDDWDDVLNIRP
                     IENIPVEALGYRIEFLEEWLKRVEKDEYWDLMDWHLQKDKINVPALVVSGWYDDNSMG
                     TTEALDVIKDYEKGKRKAILGPWMHNSNTLRDINGISLGNSSLRYDLDYNYLLWFDKY
                     LKGIENNIDTTAPVEYYSVGSNKWKTEENWPIINKIDKSMYLISDGNANTSLGNGRLV
                     FDNDLEEKYDSYIYNPKDPSVQLIDMSENEVGVPNNYKDLEKRSDMLCYTSDAFSEEF
                     TVTGDIKLEFFASSSAKDTDWVIKIMDVDLDGNSIKLADGILSARFRNSFYKSEFMEE
                     GEIYKFTIITSKISNTFKVGHKIRLDITSSAKNFIFQNSNTTEGYNSIEYIEAKNTIY
                     HGGKYPSKLILPIENK"
     gene            complement(2674..3834)
                     /locus_tag="ACOIAF_000737"
     CDS             complement(2674..3834)
                     /locus_tag="ACOIAF_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HGZ2667763.1"
                     /translation="MNTLFSNPGSILAVMTSMIALGFYLQRYKAIKSLGPALTIIIMG
                     IILSNLKVVPVSTELYGTISTYAIPVSMTIMLMSVDLKEMTKLSREPLIAIFVAVLTV
                     SIMAFLFGLVFAEKISEGWKVAGMFVGTYTGGSANLTAIGTGLNVSRQTLAAANAADY
                     VIGVPTLIFMFALPAILKNSKKFKKLWPYHVEESELEDCQNEEFMESKEWSIKDIAWM
                     LAIGFVVTEVSTILAGYFNSSFSSAARILLVTTISIIIAQLKPVKKLKGNLDLGLFVA
                     LFFLCTIGFSVDIKEFLGSTFTITLYCFSIIFASFVFHLGITRLLKIKYQYVILSIVG
                     AIADGPTSALVAASAKWNSLVSVAVVMGVIGGVLGNYAGISVAYAIKMSLGL"
     gene            complement(3853..4920)
                     /locus_tag="ACOIAF_000738"
     CDS             complement(3853..4920)
                     /locus_tag="ACOIAF_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HGZ2667764.1"
                     /translation="MKITDIKFEKLRIKLKKPVVVSFGVIEYGESIILKIETDEGYCG
                     FGEAAPLAAVTGEVLDNVLSVLLMFKKELIGKDPLDIETIHTIMDGIIIGNTSAKAAI
                     DIALYDIKGKIMNAPLYKVLGGFDSKVQTDITISIDKPEKMAVEALERVKEGFRILKL
                     KAGINPEDDIEAVKLIREAVGESIRIRIDANQGWNVNSSINTIKKLEEFNVDAIEQAL
                     PHWDLDGTAYIRNKSNTKIMIDESLHSPIDAIKAIKKNAVDTFNIKLMKSSGIYPAIK
                     INNIAEASGVNCMLGCMLETRIGITAAANLIASKKNITEADLDSFMFCEELEGISGGF
                     VMDGDIMNLVNKPGLGIEVNL"
     gene            5271..5837
                     /locus_tag="ACOIAF_000739"
     CDS             5271..5837
                     /locus_tag="ACOIAF_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGZ2667765.1"
                     /translation="MLGEKMRNIRKSKKKTLSDVSKLTDLSISYISQIERDAIEPSLS
                     SLRKIAEVLDTPLYMFMDDNNTDDLVIRKEDRVMMKFPKSEMFYEIVSPMPTTEFTPS
                     ILFVEFELKPESEDAKDYIYHASEEIMVVTSGVVDICTGEKVVRLNPGDSTFIKANVP
                     HKIINPSKDTIARGYGVISPPIWPIKSK"
     gene            complement(6056..7042)
                     /locus_tag="ACOIAF_000740"
     CDS             complement(6056..7042)
                     /locus_tag="ACOIAF_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HGZ2667766.1"
                     /translation="MGFKVELGGFQTLIQDRGRVGYGQYGVSGCGAMDEYAHRVGNIL
                     VGNSEDEASLEVLMLGPTITFDEYTQIAVTGGDLGAKINGKEIQNWRSYQINPGDVLS
                     FRGVKSGARAYVSIAGGIDVPLAMGSKSTYTRAKIGGFEGRALKKGDYINTFIQEKDF
                     TINKKLSSKYIPTYSSEIVLRIVKGPQFDAFSNGEVEKFLSNKYKVTNEIDRMGCRLD
                     GESIKHLNGADIISDGISYGAIQVPGHGKPIIMLSDRQTSGGYTKIGNVISVDLYKLA
                     QAKPNDVVKFELVDIYEAHRLLREQEDKIQDIYKSMKNIRVVKAKVLNDIAV"
     gene            complement(7072..7767)
                     /gene="pxpB"
                     /locus_tag="ACOIAF_000741"
     CDS             complement(7072..7767)
                     /gene="pxpB"
                     /locus_tag="ACOIAF_000741"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HGZ2667767.1"
                     /translation="METRYLISGDKAVVAEFGNEISEDINKKVISFMRAIEISNLKGI
                     VTEIVPTYRSLMISYNPLEIDFDSLIENLKKIEDNLESIVLPKPKIHEIPVCYDEVFG
                     IDIKNVASYNNLTVDEVIKIHTSREYLIYMLGFTPGFPYLGGMDERIATPRLEVPRTK
                     IYGGSVGIAGSQTGVYPIDSPGGWQIIGRTPLKLYDENREEQILLRAGDFIKFVPITL
                     DEFIEIEKNNLSI"
     gene            complement(7787..9007)
                     /locus_tag="ACOIAF_000742"
     CDS             complement(7787..9007)
                     /locus_tag="ACOIAF_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NRAMP family divalent metal transporter"
                     /protein_id="HGZ2667768.1"
                     /translation="MSTKNITDKTKNKKDVGALIGAAFIMATSAIGPGFLTQTAQFTQ
                     DFGPNFSFVILITTILFIGAQVNVWRVIGVSGLRGQDIANKIIPGLGYLVAFLVALGG
                     LAFNIGNVGGAALGMNVMFNMNMTLGTVLSGLIAIFVFMSKNSNSLVDKITKFLALGM
                     IIIVGYVAISNHPPVGEAVSRMVKPENPKGLIFPIITLLGGSVGGYITFAGGHRLIDG
                     GITGEENIKEITKSSLLGILVATMMRVLLFLAILAVVSKGLQLDPENPAASAFKFSAG
                     AIGYKFFGLVLWSAAITSVIGAAYTSVSFLKTLNPFIDKYEKYFIIAFIAISTLIMAF
                     IGKPATLLILAGALNGLILPITLGIMLIASKRKDIVGDYKHPTWLLIFGLIVVLISAY
                     TGITSLSSLGALFA"
     gene            complement(9036..9827)
                     /locus_tag="ACOIAF_000743"
     CDS             complement(9036..9827)
                     /locus_tag="ACOIAF_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HGZ2667769.1"
                     /translation="MYKVDLNSDLGESFGTYKIGLDEEVLKYISSANIACGFHAGDPS
                     HMEKTVQLAKKNGVKIGAHPGFLDLIGFGRREMKITKQEAKDYTKYQLGALMAFASSN
                     GCNIQHVKPHGALYNMAAKDKELAMGICEAIYEVDKDIILLGLYNSEMINSAKEIGLR
                     FANEVFADRAYDNNGFLVPRSVEGAVIHDTKHAIDRVVRMVKEGTVETLTGEVIHIKA
                     DSICVHGDNPKAIEFVKEIRKRFELESIEVCPLENIEVCSSENIV"
     gene            10415..11044
                     /locus_tag="ACOIAF_000744"
     CDS             10415..11044
                     /locus_tag="ACOIAF_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HGZ2667770.1"
                     /translation="MDKNYTMPIYQKIALDIANKIYTGEIQEDSVLFGRSVLAGKYNV
                     SPETIRRAVKILEDIGVVKSIKGKGVIVLSPDKASSFIKKYRDITNISSYKSTLYNLI
                     DTKSNLENEILDTINKILDYSNRLEIINPLVPVQFTINSNCKYIGQTAAQTKFWQNTG
                     ATIVAIKRGEELIISPGPYIEFLEGDILLVVGDQHIYNSIPMFLYENEK"
     gene            complement(11158..12672)
                     /gene="glpK"
                     /locus_tag="ACOIAF_000745"
     CDS             complement(11158..12672)
                     /gene="glpK"
                     /locus_tag="ACOIAF_000745"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HGZ2667771.1"
                     /translation="MEKKYVMALDQGTTSSRAILFNKKGEIVKIAQKEFNQIYPKAGW
                     VEHDPMEIWGSQSGVMREVIETAGIRPEEIASIGITNQRETTVVWSRYTGKPIYNAIV
                     WQCRRTSEICDELKNKGLEESIKEKTGLLIDAYFSATKVKWILDNVEGAREKAEQGEL
                     LFGTIDTWLIWNLTRGKVHVTDYSNASRTMMYNINDLEWDEDILRELNIPISMLPLVK
                     PSSYVYGHTDERMLSGAKIPIAGCAGDQQAALFGQNCVEEGTAKNTYGTGCFLLMNTG
                     SNIVKSKHGLLTTIAWGVDGKVTYALEGSIFIGGASVQWLRDELKIIESAKDSEMYAN
                     RVEDTNGVYVVPAFTGLGAPYWDMYARGSILGLTRGAKKEHIVRATLESIAYQTKDVL
                     EAMQNDSKLKLKSLKVDGGASNNNFLMQFQSDILNVDIDRPKIVETTALGAAYLAGLS
                     VGFYIGRNEITSKWSVEKEFNPNMSEEKRCKLYKGWKKAVSRALSWEKEDELDI"
     gene            12916..13458
                     /locus_tag="ACOIAF_000746"
     CDS             12916..13458
                     /locus_tag="ACOIAF_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HGZ2667772.1"
                     /translation="MPKILENVKEDILKVSRDMILEEDYSNISIRKIAQRCGISAGTV
                     YNYFNSKQEIIEYITKSEWDLLIRRIEYSNKNTEDYIKKLNIIFAEIRNFINKVHNIQ
                     YNDFLNTFETERFFEMKKHKDDFHEQLSNKVYEALEKESFFNNDKLVCNIIIKMFFSY
                     STYLHIEFEDLKPYIEKLIH"
     gene            13463..15964
                     /locus_tag="ACOIAF_000747"
     CDS             13463..15964
                     /locus_tag="ACOIAF_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGZ2667773.1"
                     /translation="MFTLNHNTSIKNKDSNPKIFLVLTLYLLGIFMGAIDTGIVSPAR
                     TIIQNSLGVNEKTGIWMITIYTLTYASVIPISGKLADKIGRKYVYLVSIFLFGSGSLI
                     CGLSSLFSNFYILLIGRVIQAIGGGGIMPIATAEFGTTFPENKRGMALGLVGATYGIA
                     NILGSSIGSTILSIFGTQNWKWLFFVNLPICLIILIGGVFCIKNNKSESKEKIDKLGT
                     LMLICIIVSLLYGLMNIDFFNFKNSIQDISVYPYLLTFIVLIPIFIFIENRAKDPILS
                     FEYFLNPRILIVLILSLIVGIGMMGMVFVPQYAENALKINAGSGGYFVAILGLFAGIA
                     APLSGRLIDKFGAKKILLLGFSVSMIGSLYLVLIALKTNTVFSVCVSLIFMGLGMGFT
                     MGTPLNYMMLSNTRLEESNSALATLSLIRSIGTSISPAIMIGFIAHAGLSVQDNIMDL
                     VGKPSTPKIVQLEELENMIDDLKSDPEMAKNLKNVSIPNMNDTSNINMDMTGGKLPKS
                     LLNKVQSADTTNITNITKEISTTMFDEKVPSIITKIQKNVQKGIDGTQSGINGIEDGE
                     NKLNSGIKGVQTGIDNMVKARAGINQGIDGVKKGIAGVDKGIKGMEQGLEKQDEAIKE
                     LTTAYNKIPTTEKTPTDNENNSNEQNQSNTSNSESDDTKNDSTENKNNNSSENKPNVN
                     EQKESLNAQIQQLKKTRDDLNTKLQKNKTQKNELSKKLRSMESQKKELQSKLNNSISQ
                     KKDMEKSLDTMQQQKKSLQAVLAKTQEVKTEIPKAFDKSKLDYINSIENNRTKIENTL
                     QSTLNSGFKQMYITVFCVNLLAFIILLFYKENKTR"
     gene            16385..16783
                     /locus_tag="ACOIAF_000748"
     CDS             16385..16783
                     /locus_tag="ACOIAF_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGZ2667774.1"
                     /translation="MDTLGKRIAYLRNSKKLTQRKLMDILKFENLGKYETGDRKPNCD
                     ILMSIADYFNVTTDWLLYGKEKVNVNSSVKEDKEDYLHVTNDEMTILNLYRQLNERDK
                     IKIEGILELKISEYKDLKKHSSNNNEDKMV"
     gene            complement(17000..17209)
                     /locus_tag="ACOIAF_000749"
     CDS             complement(17000..17209)
                     /locus_tag="ACOIAF_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667775.1"
                     /translation="MCKLDSDGVIECCRAIDDFITALSNIKSLNMERLNTLTKYSSTC
                     SILLKEGNYEGCTIVYRKMLEELKT"
     gene            complement(17241..17450)
                     /locus_tag="ACOIAF_000750"
     CDS             complement(17241..17450)
                     /locus_tag="ACOIAF_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGZ2667776.1"
                     /translation="MDARKKWIPFLGVQVKQRLIELNMTQRELAKKIGVNENYLSAIL
                     NGRRTGKKYKSSIYQLLNIEYSEDD"
     gene            17749..18168
                     /locus_tag="ACOIAF_000751"
     CDS             17749..18168
                     /locus_tag="ACOIAF_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGZ2667777.1"
                     /translation="MDTLGERIVYLRKAKNLKQYELEEMLGCDNLSKFERNIRKPNYE
                     ILKSIAEIFNVSVDWLLNGDNLSHKSDLICDSSSNYPLNSINSNEIKLLNNFRKLNDY
                     DKAKIEGMIELKLHEYEKEKDLGKIEYNKNKDEKIDK"
     gene            18396..18851
                     /locus_tag="ACOIAF_000752"
     CDS             18396..18851
                     /locus_tag="ACOIAF_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667778.1"
                     /translation="MGLRDKFAQSFARSKTMSGPEKKANEIMGKLLLKKAILPIVLMF
                     VIIIAGAMLKINSWVTLGINLVIAVGAFFYIRNSSKKYQNFKPYVGNLISLEKKGKKE
                     YVAIIKQGKLPVKLQIAYGGEDLEHVKKNQMVQISYNPDAKIAILVNRQ"
     gene            complement(19059..19319)
                     /locus_tag="ACOIAF_000753"
     CDS             complement(19059..19319)
                     /locus_tag="ACOIAF_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667779.1"
                     /translation="MDKLITELSSLGAIGILCALLFKNTMQEKKEDRDMYKKTVENFI
                     ELSTQQQEINKNILVQMGIMKTDVEEIKEDVTDIKGMLQNGV"
     gene            complement(19582..19935)
                     /locus_tag="ACOIAF_000754"
     CDS             complement(19582..19935)
                     /locus_tag="ACOIAF_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1375 family protein"
                     /protein_id="HGZ2667780.1"
                     /translation="MFKFDKNKIEQIKQGRKVEMQYKDISDISIGQVKQDDDITNNFI
                     ANAEIYEMLLNQSSVNEASNISTFSVRKSGGESGMVEVYVALILRGKKTIEEVPAVIR
                     EQVRIRCKELEIPVE"
     gene            complement(19952..25183)
                     /locus_tag="ACOIAF_000755"
     CDS             complement(19952..25183)
                     /locus_tag="ACOIAF_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome condensation regulator"
                     /protein_id="HGZ2667781.1"
                     /translation="MKQNKLLQRGAYFNDKNILIDDFDKRYNDYDFVEFFTGISNSTF
                     GLKSDGNLYACGDNTGFQLGLGKDSSERRMFSKVKIDNVKYVSCGSKHSVAVTKDGFA
                     YGAGTSNVGQLGVIESTVYYEFTKLPIDDVKTVACGYDFTFVLKNDGTLYSAGLNSSG
                     QLGLGDTNNRVTFTKVNIDSVKDVVTYNQSVFIIKMDGTAHACGLNSNGQLGINSTLN
                     KSVFNKIEGMDNVKQIACGSSHTILIKNDGTMYTTGSNGYGQLGTGNNNNSIVFTLSS
                     INNVKYASCGNNHTMILKYDNTLFSTGQNNYGQLANANKDVASRNTFVKVNVENIKDI
                     KCGSQFNFLINGSKEIFVSGCNLAGQLGSFFHTTFLYEFSKVQSSNLDNYSGLLVNDD
                     YLYVTKDNSEFLNVKLSDNFQDYKKIELTDNNMFIVMNDGTLYACGLNNYGQLGLGDT
                     VNRSVMTKVDIDNVLDIKGNGNSTFVLKNNGTLYSCGYNSSGILGLKDNTNRNIFTKI
                     EIENIKEFCVESNYIVALNHSKELYGWGNQSYIVYGDNRNYPYKDTRVSNVEKIATWS
                     DTLYILDSTGATKTIGYSYNGSGGYPAPSSSSTYREGGYINKNTSYRTLEFYNTSKTK
                     LVNLFAFYNGCVFVDENGLAYCIGENNINFRGGSTTNENNSLRFINNSGVYYTNTDGT
                     DYTCYQWTYKLIRCSIFDSPQNIIGNSKNILYLSKNNSTFKCTGNCITYGINSQNWYS
                     YFSDSSNGAIALGNEFILKNYSGECLLKGYGKATNGEFGNSTNISSISNYDTGLKDIK
                     DIIVKNNTVVVVDKNNNIYVTGANQFNKLGIGEYNNQPIRKFTNITEQSNSFIFMDDI
                     KEITTSRNTMFIVKNDGTAYATGNNSSGQLGLGDTINRNKFTQINLDNIKKISTSIDG
                     NTTFAIRNDGTLYSTGLNTKGQLGLGDIVNRNTFTKVNIQNVRDVVLGTTHSHAIKDD
                     NTLYSCGENTHGQLGLGSESNHPDVLTFTVNNITNVRDVYCSDTTTFIVKDTNIAYCC
                     GYNNNSQLGMGNTTDQYSFIKCMENVKEVIPNEINTYIITIYNTAYSTGLNTDYCLGL
                     NSNSNQSSFSEIPISNVVKVAPNRNNAVLLLTSEGDVYTAGKCSNGSGTGSETPEKIK
                     KIASKAKDIGMNYRCGHYVSDNGDLYGTGFNDCGQLGVGNVTKRDTFIKTNTRVKKIL
                     PLEYANIAIKDTNDIYICGLNNYGQLGVGNRYDSRNNDNRIFNYKHMNFVMGDLTSIK
                     NRHNFILLNNKIVIPTTKDIDYGLVLGNLYKGDLYTELPYEDIKEVSISKTHIIILLN
                     DGTMYGCGTNYHGELLQDLSINQVDEFVQINVSDVKHVSCGDNFTYFIKSDDSLWSIG
                     KNSEYQLGIGHNNPVTELQRITTISSCKEVHCGKNYTLVVTTSNELFVQGYNDKGALG
                     LGSDSENTIIKFFTKALTDIREIKSYGSDHILVLKNDNSVWVTGKNRDVYKIEQPVEF
                     LKEFTIVPISEDVNTVKDVLATDNTLYIISEVGTTNAAIEITEKSISSIKIKIQDPNK
                     DISRIEMLINGESVKSVSDLITEKISFEVPPDKIKIGENKILFRAYCKGDDLYASLFI
                     FKESTGNSIIKDSYVMIGNRMYKVVNTTSNEQDITITLDRGLEEDLNLGDPIYQLINK
                     TKVQVKINKSDLFKDMKLVEIKKSDSSYQEIYELEEANIKSAQPKIIVEKGDKWTAIK
                     RPSMIFRYDAENNEPQA"
     gene            complement(25205..26191)
                     /locus_tag="ACOIAF_000756"
     CDS             complement(25205..26191)
                     /locus_tag="ACOIAF_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGZ2667782.1"
                     /translation="MEEKFYIILTKIGREKIANATALGELVGLTKFQVGDSNGEYYEP
                     TEEQTALKNVVWEGNINSLRIDEKNPNWIVIETILPGTVGGFMIREAAVLDNENNIIA
                     IGKYPETYKPRAEDGSIKDLVVKMILQLSNTSNVTLEVDPTLVFVTQKDIQDLDDKFD
                     KNIKEIKVKIGDTDILTTDSKDLSGAINEVVKKIENISFDDVISGQIQTDISVLKNSY
                     NKLSEKVLDILIYLELESEVTVDEAGYWYDTLANGNNIVAIEGLKLDLNRKCITGEIG
                     NVIFRDVVLPFSANRVRYIHDMDNNFVETKSSNTYLKEQKDITLNKYSYEIR"
     gene            complement(26192..26890)
                     /locus_tag="ACOIAF_000757"
     CDS             complement(26192..26890)
                     /locus_tag="ACOIAF_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail protein"
                     /protein_id="HGZ2667783.1"
                     /translation="MKLIDKLPSFDRNYIVEEIQGAYDTELNILKEDIDDTFNQLFVD
                     TATWGLDMWEDILCIEKKELDFDTRRSNIKAKMRSRGTSTIEVIKSICEAYTKSETDI
                     KVYSDEFTFVLSFIANNCDYKTLLDCSDMIERVKPAHLLHYLEPIILDKSMVYCGGGM
                     VCSEEVKVHPYFEPIIKCSAVVNCGAGMLSREEIKVYPLSIKCIENNCKINIAIANDT
                     GVENVVVYPKSEVV"
     gene            complement(26887..27939)
                     /locus_tag="ACOIAF_000758"
     CDS             complement(26887..27939)
                     /locus_tag="ACOIAF_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HGZ2667784.1"
                     /translation="MYSDQTYEVIKNRTLENINLDIYKGEGSFLNNMVSGNNLELSKI
                     YLELSKMHKMAFIQDTYNQFLDKRVNEFGVYRKLGTESNGEVEFIGEKGTVINNGTII
                     SYRDLLFVVIKDVTIGSEEGDNSPVQALEVGKKYNLPTNCEFKLVDNISGVTKITNTR
                     SFEGGTDIETDEELKERFYKIQRNQATSGNKAHYEEWALEVDGVYNVKVYPRWDGPGT
                     VKVLIFGKNNQAVDTETIERCQQHIDEEKPIGPTITVVTPLPIEISISAVMKLEDGYT
                     LDNVKESFLESINTYFRDIRGEIIYTKVMGILINTTGVHDLSNLLINGSTDNITINED
                     KIPSVTTVNFSEVENQ"
     gene            complement(27932..28360)
                     /locus_tag="ACOIAF_000759"
     CDS             complement(27932..28360)
                     /locus_tag="ACOIAF_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HGZ2667785.1"
                     /translation="MSTIFPFIGVPEDYILPKTEELPIFREVAWDFEKDEPILEKGDF
                     KIIEKKEALKVWIYKCIKTNRYEHEIYSLEYGTELSELIGQKYTKGLTESEASRFIKE
                     ALLINPYILEVNVKSANFNRDILSANVKVSTIYGEVEINV"
     gene            complement(28360..28686)
                     /locus_tag="ACOIAF_000760"
     CDS             complement(28360..28686)
                     /locus_tag="ACOIAF_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 domain-containing protein"
                     /protein_id="HGZ2667786.1"
                     /translation="MANPINEFIGIIREEGKYHNQPSFFIGKIKSKLPDLKIETNNII
                     LEKEDILIDSWMIDRQLETFDTETNQEHQHEVKNPFIDNFESGDMVIMFRIGEKFAVV
                     SKLVSL"
     gene            complement(28702..30231)
                     /locus_tag="ACOIAF_000761"
     CDS             complement(28702..30231)
                     /locus_tag="ACOIAF_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HGZ2667787.1"
                     /translation="MIINRSKDSSSNEISFVSKDMGFLLTQSEVSYNFKDKLVEDIAK
                     QVFAENRLSVGTIAKTNVKYTKMFIGVNGYDTIMSAYTEASKKTKKKYMIEANLDKFN
                     VIEKGTVTLSVMFEEGFNIINTTFSESMENVKNKVIVVDQYGSKISEKIDNEIFKEVN
                     VIMQKVIQQQENQDVDIDSEFNGIEKSCSLKGYGDVSCITGRGVKVKDSYTKLVGLFY
                     IDTDKHTWQNGEYQIELELNFQNLMDEKSAGQDEPKEESNLGGEDYAGGKEFTAEFTA
                     YCPRKEEGGDTDCRKKKLDPSKKTCAAPMVGKYEQTYYTKEFLNKHPLLNYGDEIQVI
                     TGVSGRDGVYKVNDVGPAITIEKNGTYHIDILFGNVEEASKFGRRKGKIIIGGYSGNV
                     SDKAKIVISEAKKHLGKPYKWGGNGPSSFDCSGLMVYCFKKVNVSLPRTSNQQSKKGK
                     KVEQKNLQAGDLVFFHNPVSHVGLYIGNGEFLHAPQKGDVVKISKLSSRRDFNTARRV
                     L"
     gene            complement(30513..30935)
                     /locus_tag="ACOIAF_000762"
     CDS             complement(30513..30935)
                     /locus_tag="ACOIAF_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HGZ2667788.1"
                     /translation="MEMWLRQAEDRFRFPVFPSSFSINGKAAVNSSSILKIGEIATFG
                     GVALKSISISSFFPNKDYTFCDYTGFPSPYDCVNKIEKWMKEGFILRFTITETNINME
                     VIIEGFSYEERDGTRDVYFTLDLKEYKRIKIPKVTPKQ"
     gene            complement(30935..33388)
                     /locus_tag="ACOIAF_000763"
     CDS             complement(30935..33388)
                     /locus_tag="ACOIAF_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667789.1"
                     /translation="MGNVREEGINMYLTDNYTPKMNQIISVTDNFRRATVAVSLSTNV
                     MASSIKNSIGSASNRVNSLNSSLRKVQTTASSVSSTMTKLSSSINAVSGVIGSLNGSI
                     MRLAITIAMIIDYFNKLIQKKNEFNSNIMIILIFKAKSDEVEKTKNKLLGNLKKIGGK
                     IWNIVIKAKDMTKRVISSILGKLKQVEKRPYQGSINLKDMVSSAMGRILPKLMLFKNT
                     FWSGVIAIKDMASGIISKVFPKLRLFAGKVWSGAIAVKDMASGILGSIKGKISDLTNG
                     ATIGVAVKKGVDLLGQEQNQKVVLESVMKRNTGKVNQIDVDDYYGSLVRMANDTPFDP
                     EDVVAMGTKAKMISNITGGKKEKDITQAMVDVRALNMNTSSEQDVSAAFLSAAKGNME
                     SLNTLVGENYKTFDEALEGISVKQMGLAKEMSNTIPGIISGAQTSINNGLKSIVKPFD
                     DILGQGLKKIKTFIESGLGNLAGLSEKMAGKIGNVMNGKIIIGNKYDQMQSRSVKNGK
                     EFSDSTQYRISNEAEKRKMMVENKQERFENHAATMIGNAPKAIVNAGSTLLQNIDFTA
                     LIDSLLPVVNLVNNLLDSINNKSPIAQGLISIFGTIVTTAFQLIGPVVEAVSPIITRI
                     FTFLGEYAPQINNFIETLGVIWKTVWETLGPLLETGWKIIEPILGAFFNILDKVCKIV
                     KDICKWWQTMINKIKNGSITGTVLNLVEKSKKNYKDNPYAGTKAGDSGKAYSGKKGNN
                     AFGLNYVPYNDYQTRLHEGEMVLTKQEANQYRSRKNGGNINIAKLADTIVIREEADIE
                     KITSKLVASIQLAQLGGVL"
     gene            complement(33388..33555)
                     /locus_tag="ACOIAF_000764"
     CDS             complement(33388..33555)
                     /locus_tag="ACOIAF_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667790.1"
                     /translation="MYYYWKKKGIRPSLFYAMDKGELKLIEAFFALEIEEEVEKMKHG
                     YGVCPLTGGGM"
     gene            complement(33582..34028)
                     /locus_tag="ACOIAF_000765"
     CDS             complement(33582..34028)
                     /locus_tag="ACOIAF_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGZ2667791.1"
                     /translation="MSENGLLKNINIVDLLLNADTENLERPSTIVELKRLSTIFGQEF
                     KVMCRALTISKDEEIQNTCLKIDENMKTDIDLPEMQMLTIIEGVCDLDGKLLFKNKEL
                     MDKFKAPTPKELARKLLLPGEITNLYRILQDVMGYGKNAVIEEVKN"
     gene            complement(34154..34582)
                     /locus_tag="ACOIAF_000766"
     CDS             complement(34154..34582)
                     /locus_tag="ACOIAF_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HGZ2667792.1"
                     /translation="MANMEARNVMSGTWGELWLDGNKVAEVKKFQAKMEFTKEDIIIA
                     GQMGTDTKYMGYKGKGSITLYHVSSRMHKLIGEKIKRGSEPRFVAISKLNDPDSYGAE
                     RIAVKNIAFDDLTLADWEVGVKGEIEAPFTFTEYDFLDII"
     gene            complement(34596..35660)
                     /locus_tag="ACOIAF_000767"
     CDS             complement(34596..35660)
                     /locus_tag="ACOIAF_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HGZ2667793.1"
                     /translation="MAIGLPSINISFKELATTVKERSARGIIAMVLKDAKALGLNEIH
                     EKEDIPVDLSAENKEYINLALMGNVNTPNKLLVYVIEGEADIQTALDFLETKEFNYLC
                     MPKAVEADKTAIKNWIIKLRDIDKVKVKAVLGKVVGNHEGIINFTTEDVLVGEKKYSV
                     DEFTSRVAGLIAGTPLSQSVTYTKLSDVVDIPKMTKVDAESKVNKGELILIKEAGAIR
                     IARGVNSLTELTAEKGEMFQKIKIVDTLDIIHSDIRKVIIDDYIGKVTNSYDNKCLLI
                     VAIKSYLEELEKSALIESDSTVEIDFEAQKSYLKSKGVDLSYMTLQEIKEANTGSKVF
                     LKAKIKVLDAMEDIDLSIEI"
     gene            complement(35665..36108)
                     /locus_tag="ACOIAF_000768"
     CDS             complement(35665..36108)
                     /locus_tag="ACOIAF_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6838 family protein"
                     /protein_id="HGZ2667794.1"
                     /translation="MLKYKEILETIIEILKKNFTESIFIDDESVQGSEGSCFFVSILS
                     VICTPVMLNTNNKDIVISIKYLPKPQSKSIRMYEVSDELNKLFNRNIKVTDRKLNITK
                     LEQSIKKEESIYVLNFTLTLNYLDSVYEEDVVYENMEEINLNLGE"
     gene            complement(36357..36797)
                     /locus_tag="ACOIAF_000769"
     CDS             complement(36357..36797)
                     /locus_tag="ACOIAF_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667795.1"
                     /translation="MENKKDILFKETDKRLHNYKYLDIKIKNINLDIKRCENEYSGCG
                     AMVYTEKTSNTYNISSSVENEVLKREERLRKLKMEKEDIEIEKEKIENALTCLNDIEM
                     EFFNLFYNSKTKNNMTYISMKLHLDRTSCYNLKKKMIFKLSEIL"
     gene            complement(36902..37099)
                     /locus_tag="ACOIAF_000770"
     CDS             complement(36902..37099)
                     /locus_tag="ACOIAF_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGZ2667796.1"
                     /translation="MFKNNLKYYRKCKGMTQIQLARKAGITNDYISQIERGIKNPGLL
                     MAKKISSILEQNIEEVFFIQS"
     gene            37268..37588
                     /locus_tag="ACOIAF_000771"
     CDS             37268..37588
                     /locus_tag="ACOIAF_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGZ2667797.1"
                     /translation="MFAKRLRELRKEFGLTQRELGEKVGVSQRVLGYYETENRFPDEH
                     ILNKLADVFNVSVDYLLGRTLVKENIDTVAAHRKNPHEELPEEAQEQLNDYIEFLLNK
                     YKKK"
     gene            37670..38176
                     /locus_tag="ACOIAF_000772"
     CDS             37670..38176
                     /locus_tag="ACOIAF_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HGZ2667798.1"
                     /translation="MNNLDKLFELASQEGIIIHYTTYIAGDLEGLYINKHGIKIISLL
                     SNLKQNSKKLTSILAEELGHHFTSLGYYVSSYNDYYTKIIIDKCENKALKWACEFLIT
                     EEDIINIINSGITCVYEMADILNVDITFFQKRLEFLSLKKQSLQLGNNKYLILTNLPY
                     FYIFDPIS"
     gene            38877..39632
                     /locus_tag="ACOIAF_000773"
     CDS             38877..39632
                     /locus_tag="ACOIAF_000773"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HGZ2667799.1"
                     /translation="MEKKVLATVGEKEITNIDIENALKSLDPYQAMQFKTEEGKKHLL
                     NDLVNQELFFLDAKEEKLDEEEIFKLEMKKIEENVLKQFAINKVLSSVNVTEDEKVKF
                     FEANKSSFSKPESATAKHILVDSDEKAKEILAQIKSEEISFEDAALKHSSCPSKDMGG
                     DLGTFGRGQMVPEFEEAVFSMAKGEVSEPVKTQFGYHIIKLEDLQESTESTFDEVKAE
                     VEKSLLYQKQNEVYGNKINALNAKYGNLVKYND"
     gene            complement(39775..40203)
                     /locus_tag="ACOIAF_000774"
     CDS             complement(39775..40203)
                     /locus_tag="ACOIAF_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HGZ2667800.1"
                     /translation="MNILFFLTPKSEVAYIYEDYTIRQALEKMEYHKYSAIPIISKDG
                     KYVGTITEGDFLWTLKNDLNLDLKGLEDVPVTDINRKMDNSPVSINADIEDLVIKSLN
                     QNFIPVIDDQDTFIGIIKRRDVIGYCYEIIRGYKNLANDN"
     gene            complement(40624..40824)
                     /locus_tag="ACOIAF_000775"
     CDS             complement(40624..40824)
                     /locus_tag="ACOIAF_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HGZ2667801.1"
                     /translation="MKTGVVKWFNNEKGFGFISVEGEGDVFVHFSAITGDGYKTLEEG
                     QSVEFEVVDGAKGPQAANVVRL"
     gene            complement(41007..42161)
                     /locus_tag="ACOIAF_000776"
     CDS             complement(41007..42161)
                     /locus_tag="ACOIAF_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HGZ2667802.1"
                     /translation="MKILKNKNKYIKGIMAFVFLSLATTAGVFLINDLSMKNMEEKVM
                     ESANVEGDDVKDQYKLLLENLFDYRNKAILEKNENILKGLYETDKKFGLWAYENEVKK
                     MKYLENWSSKQGVKFKDIKTRVKIKKIKEKEKDLYGIICSVSTEYKYSYENQKDKENA
                     FRIGTYHYLNVKIIDNQYVITKEWYTDPFADSLNLENIKSDDIRNYIGQQDGVELQLT
                     DEQKKAIDYAHKYCGAAADEEHGMKFNPNYRDYNPEGGDCANFASQIMFESGRFKKNS
                     IWNYSERAGTKAWVNAQAFKNYALYSGRGSLIAKGSYEEVYKEAYNLRPGDFVGYEKK
                     GRITHVSTVTGLDSRGYPLVTCHNTDRMLVPWDLGWSDKSIRFHIIKINY"
     gene            42412..43233
                     /locus_tag="ACOIAF_000777"
     CDS             42412..43233
                     /locus_tag="ACOIAF_000777"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HGZ2667803.1"
                     /translation="MQKKVAAINDLSGIGKCSLSVAIPILSALKVQCCPFPTAILSSQ
                     TGYPEFTFLDFTDEMVKYSNVWKNLKVNFDSIYSGFLGSKHQIEIVANFINDYPNAFI
                     VVDPVMGDNGVMYPIFTEEMRQEIKELVKHSDLTTPNLTEACFLTGNDYTKSDYNRDE
                     LIYIAKSVSDLGPSKVVITGILEDDNILNLAYDRDNDHVFFTSVKYNNCSYSGTGDIF
                     TSILCGMLVNKHDLGVAVNTATDFIYKTINYTSQFDTDRNDGVMFENFLSDLTNI"
     gene            complement(43309..44697)
                     /locus_tag="ACOIAF_000778"
     CDS             complement(43309..44697)
                     /locus_tag="ACOIAF_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase"
                     /protein_id="HGZ2667804.1"
                     /translation="MLGGKKSNNKKVEKLRTIFIKYLSLFFIMTISIVLFLMLSFSVL
                     LSSGVILPANYAEKQFNKYKEQIISSEKVTEDIIPSIYEYGVYTLDGNLISGTFNKKE
                     SKEVWNLMRDIEERHAYSESYIKFFKKDEVFIIKYKIVSEYSSPILRAYLPKPETLGM
                     IIFSIIFFIEIVILSKVFGKKFNIEMELLKNTTEKIEQQDLDFVVESSKIYEINNVLF
                     SMDKMKLALKDSLEKQWMLEENRKEQISALAHDIKTPLTIIHGNTDLLIEINENPELL
                     EYMEYISKGVTQIEKYINTLIDISKTENGYILNKEAISMSEFIKDVLVQIEALASTKN
                     LDVEFYKQDNLPKNITIDRELLFRAIMNIISNAIDYSPSQSKLYISVSVCNKYLKFVV
                     TDCGSGFSKADLSKATEQFYTGDLSRNSKSHYGMGLYIVNNIVQKHNGILYIENSVKT
                     GGAMVTIEIPIV"
     gene            complement(44832..45590)
                     /locus_tag="ACOIAF_000779"
     CDS             complement(44832..45590)
                     /locus_tag="ACOIAF_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HGZ2667805.1"
                     /translation="MRQLIRIFCSDLIKLKRNFIILMHICIALMGMGLFLWYYKVSGA
                     DNILKIVGYLQVIAIAFPLLSGVVCSMFVEQEYYAGNYKHMLTTSNPKYLTLISKYII
                     LVCLGFVATLVSVLGFKFGISKAYFTSSFYMISILILIACNLFEYILHLFLSLRFGKG
                     TSIGVGIVEMLLSALLLTGLGSGIWQYFPCAWGVRFITIWSSFSSSKTVEYIKVESIK
                     SYQSTGLICGFVTILAFVILCIWFSKWEGKKSEE"
     gene            complement(45592..46308)
                     /locus_tag="ACOIAF_000780"
     CDS             complement(45592..46308)
                     /locus_tag="ACOIAF_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HGZ2667806.1"
                     /translation="MERFIRVEFQKMKGTFGMKLIWIVPVFCILLCGLGGGQNAAFNW
                     WYTTFLPGALTLISSLIVLKDEKSKYRGILPLPIEMKDVWTGKMIYCIICCFIASIIS
                     AIGVVFVGIIYKETISIGQNIIATILIILLFMWQIPLCMFLASKFGIFVTVLINMFFT
                     IVGVAVFSTTEMWYMFPYSIPSRLMAVVLHILPNGLPVPEGSEFLNNAVILPGVLISI
                     VLFVLFALATRVWFNNREVK"
     gene            complement(46310..47017)
                     /locus_tag="ACOIAF_000781"
     CDS             complement(46310..47017)
                     /locus_tag="ACOIAF_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HGZ2667807.1"
                     /translation="MSNYILQVKGVSKKFKEQMAVDNISLAIKSNSIYGLLGPNGAGK
                     STLLKMITGILNPTSGKIMFEGHRWTRKDLSNIGSLIESPAIYENLTARENLKVHTTL
                     LGLPDSRIEEVLETVDLRDTFKKRSGQFSLGMKQRLGIAIALLNHPKLLILDEPTNGL
                     DPIGIEELRELIRSFPKQGITVILSSHILSEVEQVVDEIGIISNGVMGYQGEVSKEQN
                     LEKLFMKVVAENRKRGE"
     gene            complement(47133..47669)
                     /locus_tag="ACOIAF_000782"
     CDS             complement(47133..47669)
                     /locus_tag="ACOIAF_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HGZ2667808.1"
                     /translation="MNKIAVSFLIIATTLLSTACMDYSISAVELVDSKESAVVKKDED
                     AKEETTSKMINSKKTTKIPIEIISKDEKIVKYLQIDEESSLKDKLRLILDTLSNEYFN
                     GLDMEVEVKEKDNLVKINLIEPDKKSRVSWKDDYLNEQNIIYTINNIIKNVIQEEDNS
                     IWIEEVEIYYNGKLIELR"
     gene            complement(47771..48841)
                     /locus_tag="ACOIAF_000783"
     CDS             complement(47771..48841)
                     /locus_tag="ACOIAF_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HGZ2667809.1"
                     /translation="MNKKLAIFAAVLLVIGIIGTTWSGILVMPSLINFGLEKEAEFKK
                     ENKLYQEKVNIDKLDIAVDNINVTIKKSSSEDVRVTTRGNNEFYKYNVTLKDKTLVVK
                     GERKYENKIKKIKNFDQLLNTSINSMFSHDYREIIIYVPNNVDINASSISSHLFVYDN
                     VASNTITYKTSYGGFSRAITENKVNRLENLNLISNNNLHLSTKSILGVKNVNIESESL
                     YISSENEDVFINNIEEYIPENVNIKEKIGRNSNYESEFYLYSDMPIAKFLDIEVPNSK
                     VRLDIPVNKYKFNCDIKSKEAIEEFNNDEEYDNDSYDYEHNEEHSNKYRNTREIKGLL
                     NKNLSNLEKEYTIKINSNSLEL"
BASE COUNT        16067 a         8351 c         5478 g        19116 t
ORIGIN      
        1 ctttttttaa tttattattt agatttttaa tagatttttt aactatggag gtatttaaaa
       61 tatggaaata aagagatatg aaggaactgg aagaatgagt agagctgtag ttcataacaa
      121 tacagtttat ttatgtggac aaactcatgc tgaaggagga gtaattgaac aaacaactga
      181 agttttagct aaaatagaag atttattaaa caaatatggt tcagataagc aacatttgct
      241 atctgttact atttatttaa gagatatgaa agactttgaa gctatgaact ctgtttggga
      301 tgcttgggtt gaaaaaggat ttgaacctgc tagagcttgt gttgaagcta gacttgctag
      361 agagcatttg ttagttgaaa tgagcgttgt tgcagctaca aaataatcaa tttagcttat
      421 aggaggctat ctcaaaacag agagataagc ctctttttca ttgttgaaat gatgaaaata
      481 tatttgattt cgtataaaag aaagtgtctc acgaactaaa aaattcattt tgagacacct
      541 cctcactatt taatctatat aaatttactt attctctatt ggcaatatca atttagatgg
      601 atatttacct ccatggtaaa tcgtattctt tgcttctata tattcaatac tattatatcc
      661 ctctgtcgtg ttgctatttt ggaaaataaa gtttttagca ctagacgtta tatctagtct
      721 aattttatga ccaactttaa acgtgtttga tatttttgaa gttataatcg taaacttata
      781 aatctctcct tcttccataa actctgactt ataaaaacta tttctaaatc tagcactaag
      841 tataccatca gctaatttaa ttgaatttcc atctaaatcc acgtccataa tttttataac
      901 ccagtcagta tctttggcag aacttgatgc aaaaaactct aactttatat cacctgttac
      961 agtaaattct tcactaaaag catctgatgt ataacacaac atatcacttc ttttttctaa
     1021 atctttgtag ttatttggaa ctccaacttc attctctgac atatcaatta gttgtactga
     1081 tgggtctttt ggattatata tataactatc atacttttct tctaaatcat tatcaaatac
     1141 taatctacca tttcctaaag atgtgttagc atttccatca cttattagat acatactttt
     1201 atctatctta tttattattg gccaattttc ttctgtcttc cacttattag aacctacaga
     1261 gtaatactct actggagcag tcgtatctat gttattttct atacctttta agtatttatc
     1321 aaaccataat agataattat aatccaaatc atatcttaat gaactatttc ctaaagaaat
     1381 accatttata tctcttaaag tatttgaatt atgcatccat ggacctagaa tagcctttct
     1441 ctttcctttt tcatagtcct ttataacatc taaggcctct gtagttccca tactattatc
     1501 atcataccac ccagatacaa ccaatgcagg tacatttatt ttatcttttt gtaaatgcca
     1561 gtccatcaaa tcccaatact catctttttc tactctcttt aaccattctt ccagaaattc
     1621 aattctatat ccaagagctt caacaggtat attttctatt ggtcgtatgt ttaaaacatc
     1681 atcccaatca tctcttacca ttttgcttct atcaacttta tttcttgata ccataaatgc
     1741 ccatgcaagc ataccagata caaaagctcc tccttttctt ggtatatcaa taaaagggct
     1801 tcctgatgtt actatactaa ctaaagcttt aagatgctta ttgcctgaag atgcagctgc
     1861 ccattggaca taaccaagat atgatgctcc aagcattcca actattccac ttgaccattc
     1921 ctgtgatact atccaattta tggtactatc tccatcttcc ctctcatgaa tcataggttc
     1981 ccacttacct tctgattcat ttcttcccct aacatcttgt attacaacag catatcctct
     2041 ttgtacatat ttataatata tttctttatc attttctttt ccatatggag ttctcataag
     2101 aattgtcggt gcttttttgg cagaatctac aaaatcaggg agatatacat ctgtagaaag
     2161 ttttatacca tctttcatat caatcatata atttccacca aatcttatag aatatatctc
     2221 ttcattttta tgagtttttt cccattcttt taatacagta tactcttcat atccatccat
     2281 aattaatata gtacaagttt ctctagataa acagataaaa cctactattt tattatctac
     2341 aataattata tctagtggga actttttgtt tctttgaacc catatatatt cattaagttt
     2401 agaatgcgtt tctcctaagt tatttataac tgtgtcttta tctgtgttta ctaagctttt
     2461 agtatataca tcaaaattat taaataaaga ctttaaatct atttttgtaa gatttaaata
     2521 atatgtattt tcttctttgc ttattggttt taaactttct attttatttg taaaaggatt
     2581 caacttttca attaataaat ctccatcaaa gattacattt ccactgtata gtccagatat
     2641 gtataattta aattttatca attaaactcc cccctataat cctagagaca tctttattgc
     2701 atatgccact gatatacctg cataatttcc taatactcca ccaattactc ccattacgac
     2761 tgctacactt acaagcgaat tccactttgc agaagcagct actaaagctg aagtaggacc
     2821 atcagcaatt gctcctacta tagaaagtat tacatattga tactttatct ttaaaagtct
     2881 agttatgcct aaatggaaaa caaatgaagc aaaaattata ctaaaacagt aaagtgttat
     2941 agtaaaagtt gaacctaaaa actcttttat atctactgaa aaacctattg tacataaaaa
     3001 gaataatgca acaaataatc ctaaatctaa atttcccttt agtttcttta ctggctttaa
     3061 ttgagcaatt ataattgaaa ttgttgtaac taaaagtatt cttgctgcac tactaaaact
     3121 tgaattgaaa tatccagcaa gtattgttga aacctctgta acaacaaaac ctatagccaa
     3181 catccaagct atatctttaa tactccattc ttttgactcc ataaattctt cattttgaca
     3241 atcctctaat tctgattctt ctacatgata tggccaaagt tttttaaact tctttgaatt
     3301 ttttaatatt gctggtagag caaacataaa tatcaatgtt ggtactccaa ttacatagtc
     3361 tgctgcattt gctgctgcaa gagtttgtct acttacatta agacctgttc caatagctgt
     3421 aagattagca cttccaccag tgtatgtacc tacaaacatt ccagcaacct tccaaccttc
     3481 tgatattttt tctgcaaata ctaatccaaa taaaaatgcc atgatactta cagttaatac
     3541 agctacaaaa attgcaatta aaggttctct tgataatttt gtcatttctt ttaagtcaac
     3601 actcatcaac attatagtca tagaaactgg gatagcatat gttgaaatag ttccatataa
     3661 ctcagtactt actggaacta cttttaagtt tgaaagtatt attcccatta tgattattgt
     3721 aagtgctggt cctaagcttt taatggcttt gtatctttgt aagtaaaaac ccaatgcaat
     3781 cattgatgtc attacagcaa gtatactacc tggatttgaa aaaagtgtat tcatatttaa
     3841 cctccaaatt tatcataagt ttacttctat tcctaaacct ggtttattga caagattcat
     3901 aatatctcca tccattacaa atcctccaga tataccttct aattcttcac aaaacataaa
     3961 actatcaagg tcagcttctg ttatgttctt tttagatgct attaaatttg ctgctgctgt
     4021 tataccaatt ctcgtttcca gcatacatcc taacatacaa tttactccac ttgcctcagc
     4081 tatgttgttg atttttatag caggatatat tccacttgat ttcatcagct taatattaaa
     4141 tgtatcaact gcattctttt ttatagcctt aattgcatct attggtgaat gtaatgattc
     4201 atctatcata atttttgtgt tacttttatt tcttatgtaa gcagtaccat ctaaatccca
     4261 atgtggaagt gcttgttcaa ttgcatcaac attaaattct tctaactttt tgatagtatt
     4321 tatactgcta tttacgttcc atccttgatt tgcatcaatt ctaattctta tactctctcc
     4381 aacggcttct ctaattaact ttacagcttc aatatcgtct tcaggattta tacctgcttt
     4441 aagcttcaat atcctaaaac cttcttttac tctttctaaa gcttctactg ccattttttc
     4501 tggcttatct atacttatag taatatccgt ttggacttta ctatcaaatc ctccaagaac
     4561 tttataaagg ggtgcattca taatttttcc tttaatatca tataaagcta tgtcaatagc
     4621 tgcttttgca gaagtattac ctataataat tccatccatg attgtatgta tggtttcaat
     4681 atctagtggg tctttgccta ttaactcttt tttaaacata agtaatactg aaagaacatt
     4741 atccaaaact tcacctgtaa ctgctgcaag aggtgctgct tcgccaaatc cacaataccc
     4801 ttcatcagtt tcaattttta aaattatact ttctccatac tcaattactc caaatgaaac
     4861 tactacaggt ttttttaatt taattctaag cttttcaaat ttgatatctg ttatcttcat
     4921 attaccctcc ttattttttg aagttataat tcaattttta aaattttata ctttaaatta
     4981 tattattact tcaatatata tgtcaagata atttactgaa aatttactct tttccaaaat
     5041 ataatgttat aatcttaatt caattaatac tcttctatta aattttttat tattattttc
     5101 tttatattaa tttaacaata attcattttc atagactttt atttggattt aattaaattt
     5161 taattgtcaa aatctactat gtattcttat tgccactact tctatgttgt gctataatta
     5221 ctttgtttta agcgaatatt ttatattaat tttattattt ggaggttctt atgctaggtg
     5281 aaaagatgag aaatataaga aaaagcaaga aaaaaacatt aagtgatgtt tccaaattga
     5341 cagatttatc tataagttat atctctcaaa tagaaagaga tgctattgaa ccatccttgt
     5401 cttcattgag aaaaatcgct gaagttttag acactccttt atatatgttt atggatgaca
     5461 ataatactga tgatttagtc attagaaaag aagatagagt tatgatgaaa tttccaaaga
     5521 gtgaaatgtt ttacgagata gtatctccaa tgcctacaac tgagtttaca ccttcaattt
     5581 tatttgtaga gtttgaactt aaaccagaaa gtgaggatgc caaagattat atatatcatg
     5641 catcagaaga aattatggtg gtaacgagtg gtgtggttga tatttgtacg ggtgaaaagg
     5701 tagttagatt aaatccaggt gactcaacat ttataaaagc taatgtacca cataaaatca
     5761 tcaatccaag taaagataca atagctcgtg gatatggagt tatatcacct ccaatttggc
     5821 caataaaatc taaataaact caatatgaca cacctacttt ttgtagataa tacaataaat
     5881 ttaaatatcg gttttatttt aaaattgaag tatttattta aatttaaaat atttactcaa
     5941 actaaaaaaa tagctatttg actaatctaa accatcttaa ttatggtaaa tagtaaatag
     6001 ctatttgtaa gttatattat aaattataat aaaaattgta tttgttaaaa ttaaattaaa
     6061 ctgctatatc atttaatact tttgccttta caactcttat atttttcata ctcttgtaga
     6121 tatcttgaat cttatcttct tgctctctca atagtctatg tgcttcatat atatctacaa
     6181 gttcaaattt tactacatca tttggctttg cttgtgctaa cttatacaaa tctacactta
     6241 taacgttccc tatcttagta tagccaccac tagtctgtct atctgataac attataatcg
     6301 gttttccatg accaggtact tgtatagctc cataagaaat accatcagat ataatatctg
     6361 ctccattaag gtgctttata ctctctccat ctagtctaca acccattcta tctatttcat
     6421 tcgtaacttt gtatttattt gataaaaatt tctctacttc accgttagaa aaagcatcaa
     6481 attgaggacc ttttacaatt cttagtacga tttctgaact ataagttgga atatattttg
     6541 aacttaattt tttatttatt gtaaaatctt tttcctgaat aaaagtattt atataatctc
     6601 cttttttaag tgctctacct tcaaatccac caattttagc tcttgtataa gttgacttac
     6661 ttcccattgc aagtggaaca tctattccac cagctattga cacataggct ctagcacctg
     6721 atttcacacc tctaaatgat aatacatcac ctggatttat ttgatatgac ctccagtttt
     6781 gaatttcttt tccatttatt ttagccccta aatctccccc agttactgct atttgcgtat
     6841 attcatcaaa ggtaattgta ggacctaaca ttaaaacttc taaggaagcc tcatcttcag
     6901 aatttcctac taatatattt cctactctat gtgcatactc atccattgct ccacatccag
     6961 atacaccata ttgaccatag ccaactctac ctctgtcttg tattaatgtt tggaaacctc
     7021 ctaattcaac tttgaatccc atatatacca ctccctgtac tataacgata tttaaatact
     7081 taagttattt ttttcgattt ctataaattc gtctaatgtt attggaacaa attttataaa
     7141 atctccagct ctaagcaaaa tctgttcttc tctattttca tcatataatt ttaaaggtgt
     7201 cctaccaatt atttgccatc caccaggact atcaatagga tatacaccag tctgggaacc
     7261 tgctattcca acactcccac cataaatttt agtccttggc acttctaatc taggtgtagc
     7321 tattctttca tccataccac ctagatatgg aaatccagga gtaaacccta gcatatatat
     7381 caagtactct ctgcttgtgt gtatttttat aacttcatct acagtgagat tattatagct
     7441 tgcaacattt ttaatatcta taccaaaaac ctcatcataa catactggaa tctcatgtat
     7501 tttaggtttt ggtaatacta tactttccaa attatcttct atttttttta aattctcaat
     7561 caaactatca aaatctattt ctaatggatt ataacttatc ataagtgacc tatatgttgg
     7621 tactatttct gttacaatac ctttaagatt cgatatttct attgctctca taaatgaaat
     7681 tacttttttg tttatatctt ctgatatctc atttccaaat tcagcaacca ctgccttatc
     7741 ccctgatatc aagtatctag tttccataaa tgtcacctct tctaaattaa gcaaataaag
     7801 cacctaggct gctaagagaa gttattcctg tatatgcaga tattaaaact actattaatc
     7861 caaatattaa aagccatgta ggatgcttgt aatctccaac aatatctttt cttttagatg
     7921 ctataagcat aattcctaat gtgattggaa gtatcaatcc atttaaagct cctgctaaaa
     7981 ttaataaagt agctggtttt cctataaatg ccattataag tgttgatatt gctataaatg
     8041 ctattataaa atatttttca tatttatcta taaaaggatt tagtgtcttt aagaaagaaa
     8101 ctgatgtata agcagctcct ataactgatg taattgcagc agaccataat acaagtccaa
     8161 agaatttata tcctattgct cctgcactaa atttaaaagc tgaagcagct ggattttctg
     8221 ggtcaagttg aagacctttt gaaaccactg ccagtattgc taaaaataaa agaactctca
     8281 tcattgtggc aactaatata cccaaaagag aagatttagt tatttctttt atattttctt
     8341 ctcctgttat tccaccgtct attaatctat gtcctccagc aaaagttata taacctccaa
     8401 cacttcctcc aagtaaagtt attattggga atatcagtcc ttttggattt tctggtttta
     8461 ccattcttga tactgcctct ccaacaggag gatgatttga tattgctaca tatccaacta
     8521 taattatcat tcctaatgct aaaaatttag ttatcttatc gactagagaa tttgaatttt
     8581 ttgacataaa tacaaatata gctattaatc cacttaatac agttcctaat gtcatattca
     8641 tattaaacat tacattcata ccaagtgcag ctccaccaac atttcctata ttaaaagcta
     8701 atcctccaag agctactaaa aaagctacta aatagcctaa tccaggtatt attttatttg
     8761 ctatatcctg acctctaagt cctgatacac caattactct ccatacattt acttgagccc
     8821 ctataaaaag gattgttgta attaaaatta caaatgaaaa attcggtcca aaatcttggg
     8881 taaattgtgc agtctgagtt aaaaaccctg gtccaattgc tgatgttgcc attataaatg
     8941 cagcccctat aagtgctccg acatcttttt tatttttagt tttatctgta atattttttg
     9001 tactcatatt ttatctcccc ctcatgttat cagtcttata ctatgttctc tgacgaacaa
     9061 acttctatgt tttctaatgg acaaacttct atactttcta attcaaaacg ctttcttatt
     9121 tctttgacaa actctattgc ttttggattg tccccatgta cacatataga atctgctttg
     9181 atatgtataa cttcacctgt taaagtttct acagttcctt cttttaccat cctaacaacc
     9241 ctatctattg catgttttgt atcatgtata actgctcctt ctacacttct tggaaccaaa
     9301 aaaccattat tatcataagc tctatctgca aatacttcat ttgcaaacct aagtccaatt
     9361 tctttagcag aatttatcat ttcactatta tatagcccca acagtataat atctttatcg
     9421 acttcatata tagcttcaca aatccccata gctaactctt tatctttagc tgccatattg
     9481 tataatgctc catgtggttt tacatgttgt atattacatc catttgaact tgcaaatgcc
     9541 attaaagcac ccaattgata ttttgtataa tctttagctt cctgctttgt tattttcatc
     9601 tcacgtcttc caaatcctat taaatctagg aaacctggat gtgcgcctat tttcactcca
     9661 ttttttttag ctagctgtac tgttttttcc atatgactag ggtcacctgc atgaaaccca
     9721 caagctatat tagctgatga aatatatttt aaaacttctt catctaaacc aatcttatac
     9781 gttccaaaac tttctcctaa atcactattt aaatctactt tatacaattt atgacccctc
     9841 ctcgctaaca ttttgataga ataagtagtt ttaattttta aattttgttt attttatata
     9901 ttgcatgaaa tattttatca cttgtattca attttatttt tgcaactatt ctattgcatt
     9961 taattaatat aattttacca tttaaggaat atttttgcaa ttattttaat tgaacattca
    10021 tttgtatttc tttttcaaaa aatcttttta ccatttttta gttaataatt aattattttc
    10081 acttaaaata agggctttaa ccttcatttt taaagtattt ttcattttta atcactttat
    10141 atgcaagaca gaatcatttt tcttcattca tttgtttctt tcatctatat aattatcatt
    10201 ctttgtaagt aaaatatctt tatattttta taattaattc tttatatagt tttaagtata
    10261 ataaaatcat gtatactaaa tgcatgttaa aatattattt taaaacatgc acaatatttg
    10321 tacatattgt agacatataa acctataaat tatacaatag tattgttaag attatatatt
    10381 tttttaacat aaattttgta ttgggggaaa aacaatggat aaaaactata cgatgccaat
    10441 ataccaaaaa atagcgttgg atattgcaaa taaaatatac acgggtgaaa tacaagagga
    10501 ttcagtacta tttggacgct ctgttttggc tggtaaatac aatgtatctc cagaaacgat
    10561 aagaagagct gtcaaaattt tagaggacat tggcgttgtt aaaagcataa agggtaaagg
    10621 tgttattgta ctatctccag acaaagcttc ttcttttata aaaaaatata gagatattac
    10681 aaatatatca tcatataaat caacacttta taatttaata gatactaaat ctaatttaga
    10741 aaatgaaata ttagatacta taaataaaat tttagactat tctaacagac ttgaaataat
    10801 caatccttta gtgcccgttc agttcaccat aaattctaat tgcaaatata ttggtcaaac
    10861 agctgcacaa actaaatttt ggcaaaatac aggtgctaca atagttgcaa ttaaaagagg
    10921 tgaagaactt ataatatctc ctggaccata tattgaattt ttagaaggag acattctctt
    10981 agtagttggt gaccaacaca tttacaattc tataccaatg tttttatatg aaaacgaaaa
    11041 ataaattata taatttagtg tgattatata aaaatgcaaa attaacaaca aaaaaagagc
    11101 ttcatatttt acataaagct ctttttaaat tcaaataaat ttaactttga ttaattttta
    11161 aatatccaat tcatcttctt tttcccagct taaagctctc gatacagctt ttttccatcc
    11221 tttatataat ttacatctct tctcttcact catatttgga ttaaactctt tttctactga
    11281 ccactttgaa gtaatttcat ttcttccaat ataaaatcca acagacaacc ctgctaaata
    11341 agcagctcct aaagctgtag tttctactat tttgggtcta tctatatcta catttaatat
    11401 atctgattga aattgcatta aaaaattatt attacttgct ccaccatcaa cttttaatga
    11461 ttttagtttt aattttgaat cgttttgcat tgcttctaaa acatcctttg tctgataagc
    11521 aattgattcc aatgtggctc ttactatatg ttcttttttt gctcctcttg taagtcctaa
    11581 aattgaaccc ctagcataca tatcccaata tggagctcca agacctgtaa atgctggcac
    11641 tacatataca ccattagtat cctctaccct atttgcatac atttcactat cttttgcaga
    11701 ttcaattatc tttaattcat ctctaagcca ttgaacagat gctcctccaa taaatatact
    11761 tccttctaat gcatatgtta cttttccatc aactccccaa gcaattgttg taagtaaccc
    11821 atgcttagat ttaactatgt tactgcctgt attcataagc aagaaacaac ccgttccata
    11881 agtatttttt gcagtacctt cttcaacaca attttgacca aataatgctg cttgttggtc
    11941 tccagcacat cccgcaatag gtattttagc tcctgataac atgcgctcat ctgtatgacc
    12001 ataaacatag ctagaaggtt ttactaatgg caacatgctt attggtatgt tcaattctcg
    12061 taatatatcc tcatcccatt ctaaatcatt tatattgtac atcattgttc tagatgcatt
    12121 tgagtaatct gttacatgta cttttccacg agttaaattc catattaacc atgtatctat
    12181 agttccaaat aataactctc cttgttctgc tttttcccta gctccttcta cattatctaa
    12241 tatccatttt acttttgttg ctgaaaagta tgcatctatt aaaagacctg ttttttcttt
    12301 tattgattct tccaatcctt tatttttaag ttcatcacat atttctgacg ttctcctaca
    12361 ctgccatact attgcgttat aaataggttt accagtatac ctactccaaa caaccgtagt
    12421 ctctctttga ttagttatcc ctattgaagc aatttcttct ggtcttatac ctgctgtttc
    12481 tataacttct ctcattaccc cactttgtga cccccatatt tccatagggt catgctctac
    12541 ccatccagct tttggataaa tttgattgaa ctctttttgt gcaattttca ctatttcgcc
    12601 ttttttatta aatagtatag ctcttgaact ggttgttcct tggtctaatg ccattacata
    12661 cttcttttcc atcaccatat tctccttatc atataatatt taatttatta aaaagaaaaa
    12721 tttaatatca catatttcaa taaataatca tacttaaata caaatttaat ataatagata
    12781 tttagtaaga agacacttat tttctatcat atctatataa taatattata cattatagct
    12841 tacatattga ttaataaatt ttaaaggagt ataataaaca gtgttcataa taattttaat
    12901 aaaagaggtg gtaatatgcc aaagatttta gaaaatgtaa aagaagatat attaaaagtg
    12961 tctcgggata tgattttaga agaagattac tcgaatatta gcataaggaa aatagctcaa
    13021 aggtgtggta taagtgcagg gacagtgtat aattacttta attcaaaaca agaaataata
    13081 gaatatatca caaaatctga atgggattta ttgataaggc gaattgaata ctccaataaa
    13141 aacactgaag attacataaa aaaactaaac attatttttg ctgaaataag aaattttata
    13201 aacaaagttc ataatatcca atacaatgac tttttaaaca cttttgaaac agagagattt
    13261 tttgaaatga aaaaacacaa agacgacttt catgaacagc tttcaaataa agtatatgaa
    13321 gctttagaaa aagaaagttt ttttaataat gacaaattag tatgcaatat cataataaaa
    13381 atgttttttt catattcaac atatcttcat atagaatttg aagatttaaa accctatatt
    13441 gaaaaattaa tacattaagg tggtgtttac cttgaatcat aatactagta ttaagaacaa
    13501 ggacagtaat ccaaaaatat ttttagtact tacactttat ctattaggta tatttatggg
    13561 agctattgat actggaatag tatcaccagc aagaacgatt atacaaaata gccttggagt
    13621 aaatgaaaaa acgggaattt ggatgattac catatacaca ttaacatacg cttctgtaat
    13681 tcctatctct ggaaaattag cagataaaat aggaagaaaa tatgtgtatc tagttagtat
    13741 atttctattt ggtagtggtt cattaatatg tggattatca tcactatttt ccaatttcta
    13801 tattttatta attggtcgtg ttattcaagc aattggtggt ggtggaatca tgccaattgc
    13861 tacagcagaa tttggaacta ccttcccaga aaataaaaga ggcatggctt taggtttagt
    13921 tggggcaaca tatggtattg ccaatatttt aggttcaagc attggtagca caattctaag
    13981 tatctttgga actcaaaatt ggaagtggct attttttgta aatcttccaa tttgtttaat
    14041 catacttatt ggtggagttt tctgtattaa aaacaataag tctgaatcta aagaaaaaat
    14101 agataagctt ggtacattga tgttgatatg tataatcgta tcactattat atggtcttat
    14161 gaatatagac ttttttaatt ttaaaaattc tatacaagat atatcagttt atccatattt
    14221 attgacattt attgtactca tacctatttt catatttatt gaaaatagag ctaaagaccc
    14281 tattttaagc tttgaatatt ttttaaatcc gagaatattg atagtactta ttttatcttt
    14341 aattgttggt attggaatga tgggtatggt attcgttcca cagtatgctg aaaatgcatt
    14401 aaagataaat gctggttctg gtggatattt tgtagctata ttgggattat ttgcaggtat
    14461 tgctgctcct ttaagcggaa gacttattga caagtttggg gcaaaaaaaa tactattact
    14521 aggattttct gtatctatga taggttctct ttacctagtt ttaattgctc taaaaaccaa
    14581 tactgtattt agtgtatgtg taagtttaat atttatggga ttaggaatgg gatttactat
    14641 gggaactcct ctaaactata tgatgttatc aaatacaaga ttagaagaat ctaattcagc
    14701 acttgcaaca ttatccttga tacgctcaat tggtacttca atttctccag ctataatgat
    14761 tggatttata gcacatgcag gattatctgt gcaagataat attatggact tagttggaaa
    14821 gccctccact cctaaaatag ttcaacttga agaacttgaa aacatgatag atgacttaaa
    14881 atcagaccct gaaatggcaa aaaatttaaa aaatgtctct atcccaaata tgaatgatac
    14941 ttcaaatata aatatggaca tgacaggtgg caaacttcca aaatccttac ttaataaagt
    15001 tcagtcagca gatacaacca acattaccaa tataacaaaa gaaatatcaa caacaatgtt
    15061 tgatgaaaaa gttccatcta taattactaa aatacagaaa aatgtacaaa aaggaataga
    15121 tgggacacaa agtggaataa acggtattga agatggtgaa aataaactaa atagtggtat
    15181 taaaggagtt caaactggta ttgacaatat ggtaaaagct agagctggta ttaatcaagg
    15241 aatagatggt gtaaaaaaag gtattgctgg tgttgataaa ggtataaaag gtatggaaca
    15301 aggtcttgaa aaacaagatg aggccatcaa ggaacttaca actgcttata ataaaatacc
    15361 tactacagaa aaaactccta ctgataacga aaataactca aatgaacaaa accagagtaa
    15421 taccagtaat tctgaatcag acgacactaa gaatgattct acagaaaata agaataacaa
    15481 ttccagcgag aataaaccaa atgtaaatga acaaaaagaa agtttaaatg ctcaaataca
    15541 gcaactaaaa aagacaagag atgacttaaa tacaaagtta caaaaaaata aaactcaaaa
    15601 gaacgaatta agtaaaaaac taagatcaat ggaatctcaa aagaaagagc tacaaagcaa
    15661 gttaaataat tctataagcc aaaagaaaga catggaaaaa agccttgata caatgcaaca
    15721 gcaaaaaaaa tcactccaag cagttttagc aaagacacaa gaggtaaaaa ctgaaatacc
    15781 aaaggctttt gataaatcaa aattggatta tataaattcc atagaaaata atagaacaaa
    15841 aatagaaaac acactacaat ctactctaaa ctcaggattt aaacagatgt atataacagt
    15901 attctgtgta aatctactag catttataat attattgttc tataaagaaa ataaaactag
    15961 ataaaagagt gttatagtat tatattgtaa atttatctta ttcttaaata ttttattcta
    16021 aagaataaat tcataaaatt tttgtaattg attaatataa attacaatat aatactattt
    16081 ttctcttaat ctttttctct tttacagaca atcaaaaagc aatcttagtt tatcgaaata
    16141 aatttataca atctattcat acaccccttt acatttttat aacaattaaa cctattgtta
    16201 tatatacaaa tcaaaataaa ccatatattt tcaactataa tatgttaaaa tcatattatg
    16261 agcatattaa tttaagcaaa atgtcattta gactttaatt tagtagtttt atattatgaa
    16321 tttatatatt tttaatttta aatgtcattt agacattact aataaaaaga aaggagtgta
    16381 ttttgtggat actttaggaa aaagaattgc ttatttgaga aactccaaaa aacttactca
    16441 gcgcaaactt atggatattt taaaatttga aaacttaggc aagtatgaaa caggagatag
    16501 aaaacctaat tgtgatatac taatgtcaat agctgattat tttaatgtta cgactgactg
    16561 gcttctctat ggtaaagaga aggtaaatgt caatagtagt gtaaaagaag ataaagaaga
    16621 ttacttacat gttactaatg atgaaatgac gatattaaat ctatacagac aactaaacga
    16681 acgtgataaa ataaaaatag aaggaatact tgaacttaag atatctgaat ataaagactt
    16741 aaaaaagcat tcctctaata ataatgaaga taaaatggtt taaacataca taatttgata
    16801 cgattttaca tcatctatta accttctttt tttgtagtat attactaaaa tatggatatt
    16861 ggattattta aaaacaagta tagtctaatt tcaagcacat ttataatatt tagattatct
    16921 tatccatatt ttagtttaag tactttgaaa aatataatac atctctatgt ataacttata
    16981 ttcctaagaa atgcattact catgttttta attcttccaa catctttcta tacacaattg
    17041 tacatccttc ataattcccc tctttaagaa ggattgaaca tgtactagaa tatttagtta
    17101 aagtgtttaa tctttccata tttaagcttt ttatattact gagtgctgta ataaaatcat
    17161 caattgctct acaacactct ataaccccat ctgaatccaa tttacacaca agaatattca
    17221 cctactttat atactattta ttaatcatct tctgaatatt ctatattaag taattgataa
    17281 attgatgatt tatatttttt acctgttctt cttccattta aaatagctga caaatagttt
    17341 tcattaacac ctattttctt cgctaattcc ctttgagtca tatttaattc aataagtctt
    17401 tgcttgactt gcactcccaa aaaaggtatc catttttttc gagcatccat ttatcaactt
    17461 cctttctaag tgatttatat ttacttttat attattttag cttacacacc ccctctattt
    17521 agtatatatt tttaatatgt acacatatta agtatataaa tttatttaat actaaataag
    17581 attttttgat tactttgtgc taaaattagg atgtaaattc gtatcattaa gttaattata
    17641 aagtcatttt gaccttaagt caatggtttt tatgtcattt tgacctttaa tttgaaacta
    17701 tttacatctt aaaaagtctt tttgacatta attgaatgga gagtgtttat ggatacttta
    17761 ggagaaagaa ttgtttattt gagaaaagca aaaaacctta aacaatatga actagaagaa
    17821 atgttgggct gtgacaactt aagtaaattt gagagaaaca ttagaaaacc aaattatgaa
    17881 atattaaaat ctatagctga gatatttaat gtttcagtag actggctatt aaatggagat
    17941 aatctatcac ataaatcaga tttaatttgc gattcctcct caaactatcc acttaatagc
    18001 ataaactcta atgaaataaa gcttttaaac aactttagaa agttaaatga ttatgataaa
    18061 gctaaaatag aaggtatgat tgaattaaaa cttcatgaat atgaaaaaga gaaggattta
    18121 ggaaaaattg agtataacaa aaataaagat gagaaaatag ataaataatt tttatgttgc
    18181 ttttgatacg attttacatc atttctttgt tctaatattt ttatttcatt tattttcttt
    18241 tttataattt tatgataaaa taattagtat attttgcttt acttgaatgt aattatttat
    18301 acaaaagtca aacataatat aaatgtagta aatttttagt ttcaagtaat atctcgttat
    18361 tataactaaa tatttattaa aattaggagg ataaaatggg cttaagagac aaattcgcgc
    18421 aatcatttgc tagatcaaaa actatgagtg gccctgagaa aaaggctaat gaaataatgg
    18481 gtaaattgct tcttaagaaa gctatattac ctatcgtttt aatgttcgtg ataataatcg
    18541 ctggagctat gcttaaaatc aattcttggg ttacacttgg cataaattta gttattgctg
    18601 ttggtgcttt cttctacata agaaattcta gtaaaaagta tcagaacttt aaaccttatg
    18661 ttggcaattt gattagttta gagaaaaaag gtaaaaaaga gtacgttgct attataaaac
    18721 aaggtaagtt accagttaag ttacaaatag cttatggtgg tgaagacctt gaacatgtta
    18781 agaaaaatca aatggttcag ataagttata atccagatgc taaaatagct attttggtta
    18841 atagacaata atttaataaa aaaaaggagt tgccttaaaa ttgatttttt ctaatcaaat
    18901 ttaagctcaa ctcctttttt aatttttata tattattttt attacttttt ttaattaata
    18961 tttttaaata tcaaatcctt ctcaataaaa tagtgaacta tctacaatac tactttatct
    19021 tttcctaaca atatataatc tggtgctact ctttcatgtt atacaccatt ttgtaacata
    19081 ccttttatat cagtaacatc ttccttaatt tcctctacat ctgttttcat tatacccatt
    19141 tgaacaagta tatttttgtt tatttcttgt tgttgtgtag ataattctat aaaattttct
    19201 acagtttttt tatacatgtc tctatcttct tttttctcct gcatagtatt tttaaataat
    19261 agagcacata gtatacctat tgcccctaga ctactcaatt cggttattaa tttatccatt
    19321 atagcctcct attgtctaaa ttttttatac ttatagcttt aatttttgtt gtattcttac
    19381 atcatgcaat attatatttt caggattatc aatttaatct tctgtacttt aaattgtagg
    19441 tgtaatttat agattgaata aataataaat atatatacaa gttctaatgt aacaaaaaat
    19501 aagtacaaag cttacttatt aaagttaaga ccttgtactt tacataaaaa aaataactaa
    19561 tacatagtta ttctaaattt actattcaac tggtatttct aattctttac atctaattct
    19621 aacttgctct ctaattactg ctggtacttc ttctattgtt tttttgcctc ttaaaattaa
    19681 agctacatat acttctacca ttccactctc acctccagat tttcttacac taaaagtgct
    19741 tatattactt gcttcattga cagaactttg atttaacaac atctcatata tttctgcatt
    19801 tgctataaaa ttatttgtta tatcatcatc ttgctttact tgacctatac ttatgtctga
    19861 aatgtcttta tactgcattt ctacttttct accttgtttg atttgttcta ttttattttt
    19921 atcgaattta aacaattttc atacctccat tttaagcttg tggctcgttg ttttcagcat
    19981 catatctaaa aatcatagat ggacgtttta tagctgtcca tttatctcct ttttctacta
    20041 tgattttagg ctgagcactt tttatgttgg cttcttctaa ttcatagatt tcttggtaac
    20101 ttgagtctga ttttttgatt tcaactagtt tcatgtcttt gaataagtca gatttattta
    20161 tttttacttg aactttagtt ttatttatta attgatatat agggtctcca agatttaaat
    20221 cttcttcaag ccctctatct agtgtaattg taatatcttg ttcattagat gttgtattaa
    20281 ctaccttgta cattctatta cctatcataa cataagaatc tttaattata gaatttccag
    20341 tactctcttt aaaaataaat aaagatgcat ataaatcatc acctttacaa taagctctaa
    20401 atagtatctt attctctcct attttaattt tatctggtgg tacttcaaat gatatttttt
    20461 cagtaattaa atcacttaca gattttacac tttcaccatt tataagcatt tctattctac
    20521 ttatatcttt attagggtct tgtattttta tcttaattga tgaaattgat ttttcagtaa
    20581 tttctatagc agcatttgtc gttcctactt ctgatataat atataatgta ttgtctgttg
    20641 caagtacatc ctttactgta tttacatctt cagaaatagg tactatagta aattctttta
    20701 aaaattctac tggttgttca attttatata catccctatt ttttccagta acccatactg
    20761 aattatcatt tttaagtact aatatatggt cacttccata agattttatt tctcttatgt
    20821 ctgttagtgc ttttgtaaag aacttaatta tagtattttc actatcgctt cctaatccta
    20881 aagctccctt atcattatat ccttgtacaa ataattcatt acttgtagtt actactaatg
    20941 tatagttttt accacaatgt acttctttac agctagatat agttgtaatt ctttgtaatt
    21001 cagtaactgg attattgtga cctataccta attgatattc ggaattttta ccaatagacc
    21061 aaagactatc atcagatttt ataaagtaag taaagttatc tccacatgaa acatgcttta
    21121 catctgatac attaatctgc acaaattcat ccacttgatt tatagacaag tcttgcaata
    21181 attctccatg gtagtttgta ccacatccat acattgttcc atcattaagt aatataataa
    21241 tatgagtctt agaaatagat acttctttta tatcttcata tggaagctca gtataaaggt
    21301 ctcctttgta taaatttcct aatactaaac cataatctat gtcttttgtg gtaggtataa
    21361 ctatcttatt gtttagaagt ataaagttat gtctgttttt aatagatgtc aaatctccca
    21421 ttacaaaatt catatgctta taattaaata ttctattatc attatttcta ctatcgtatc
    21481 tatttccaac acctaattgt ccatagttat ttaatccaca aatatatata tcattagtat
    21541 cttttattgc tatatttgca tattctaaag gaagtatttt ctttactctt gtattggttt
    21601 ttataaatgt atctcttttt gttacattac caacacctaa ttgcccacaa tcattaaaac
    21661 ctgtaccata taggtctcca ttatcactta cataatgtcc acatctataa ttcattccaa
    21721 tgtcctttgc ctttgatgct atttttttaa tcttctctgg agtctcactt cctgtacctg
    21781 aaccattact acatttacct gcagtatata catccccttc acttgtaagt aaaagtactg
    21841 cattatttct gtttggagct acttttacta catttgaaat tggaatttca gaaaatgaac
    21901 tttgattgct attactattt agacctaagc aataatcagt atttaaacct gtactatatg
    21961 cagtattata gattgttatt atataggtat ttatttcatt tggtataact tcttttacat
    22021 tttccataca ctttataaaa ctatactggt cagtagtatt tcccatacct aattgtgaat
    22081 tattattgta tccacaacaa tatgcaatgt ttgtgtcctt tacaataaat gttgttgtat
    22141 ctgagcagta cacatctctt acattagtta tattgtttac agtaaatgtc aatacgtctg
    22201 gatggttgct ttcgcttcct aagcccagtt gcccatgagt gttttctcca catgaatata
    22261 atgtgttatc atctttgatt gcatgcgagt gagtagtccc taaaacaaca tctcttacat
    22321 tttggatgtt tactttggta aatgtatttc tatttactat atcacctaat cccagttgtc
    22381 ctttggtatt taatcctgtg gagtatagtg ttccatcatt tctaattgca aatgttgtgt
    22441 taccatctat acttgttgat attttcttta tattatcaag gtttatctga gtgaacttat
    22501 ttctatttat tgtgtcacct aatcctaatt gtccagaact attatttcct gtggcatagg
    22561 ctgttccatc attttttact ataaacattg tatttcttga tgttgtaatt tcttttatat
    22621 catccataaa tataaatgag tttgattgtt cagttatatt tgtgaatttt cttattggtt
    22681 gattgttata ttctcctata ccaagtttgt taaattgatt tgctcctgtt acatatatat
    22741 tgttgttttt atctactact actacagtgt tattttttac aattatatct tttatatctt
    22801 ttaatcctgt atcataatta ctaatactac ttatattagt tgaatttcca aattctccat
    22861 tagtagcttt accataccct tttaacaaac attccccaga ataatttttt agtataaact
    22921 cattacctaa tgctattgca ccattactgc tatcactaaa ataactatac caattttgac
    22981 tatttattcc ataagttata caatttcctg tacatttaaa tgtagaatta ttttttgata
    23041 agtataatat gtttttacta tttcctataa tattttgggg actatcaaaa atagaacaac
    23101 gaattaattt atatgtccat tgatagcaag tatagtctgt tccgtctgta ttagtataat
    23161 atacacctga attattgata aatctgagac tattgttttc atttgttgtt gaaccgcctc
    23221 tgaaattaat attattttcc cctatacaat aagctaatcc attttcatca acaaatacac
    23281 agccattata aaaagcaaat agattcacta atttagtttt acttgtatta taaaattcca
    23341 aagttctgta tgatgtattc ttattaatat acccaccttc cctatatgtt gaactagaac
    23401 taggagctgg gtatcctcca ctaccattat aagagtatcc tatagtctta gtagctccag
    23461 ttgaatctaa tatatacaaa gtatcactcc acgtagctat tttttctaca ttactaactc
    23521 tagtatcttt gtaagggtag tttctattat caccataaac tatataagat tgattacccc
    23581 acccatataa ttcctttgaa tgatttaatg ctacaatata gttactttct acacaaaatt
    23641 ccttaatatt ttctatctca atttttgtaa atatatttct atttgtatta tcttttaatc
    23701 ccaatatacc tgaactatta tatccacaag aatataaagt tccattattc ttaagtacaa
    23761 aagttgagtt tccatttccc tttatatcca aaacattatc tatatccacc ttagtcataa
    23821 ctgacctgtt aacagtatcc cccagtccta actgaccata attatttaaa ccacaagcat
    23881 acaatgtacc atcattcata acaataaaca tattattatc tgttaactct atcttcttat
    23941 aatcctgaaa attatcactt aactttacat ttaaaaattc actattatcc tttgtaacat
    24001 acaaataatc atcattaacc aataaacctg aataattatc taaatttgaa gattgcacct
    24061 ttgaaaactc atacagaaaa gttgtatgaa aaaatgaacc aagttgacct gctaaattac
    24121 agccagatac aaatatctcc tttgaaccat ttattaaaaa attaaattga gaaccacact
    24181 taatatcttt tatattttct acattaacct taacaaaagt atttcttgat gctacatctt
    24241 tattggcatt ggctagttga ccataattgt tttgtcctgt actaaatagt gtattatcgt
    24301 attttaatat catagtatga ttatttccac aagaagcata cttaacatta tttatactag
    24361 aaagagtaaa tacaattgaa ttattattat ttcctgtacc aagttgacca taaccattag
    24421 aacctgtagt atacatagtt ccatcattct taataagaat tgtatgacta ctaccacacg
    24481 ctatctgttt tacattatcc ataccttcta ttttattaaa tacactttta tttaaagtac
    24541 tattaattcc caactgccca tttgaattta atccacatgc atgtgctgtc ccatccattt
    24601 ttatgatgaa tacagattga ttataagtca ctacatcttt tacactatct atatttactt
    24661 tagtgaaagt aactctattg ttagtatcac ctaatccaag ttgaccactt gagtttaaac
    24721 ctgctgaata taatgttcca tcatttttaa gtacaaatgt aaaatcataa ccacatgcaa
    24781 cagtttttac atcatctatt ggtagcttag taaattcata atatactgta gactcaatta
    24841 cacctaattg acctacatta cttgttcctg ctccatatgc aaatccatct ttagttactg
    24901 ctacactgtg ttttgaacca caagatacat attttacatt atcaattttt actttactaa
    24961 acatccttct ctctgacgaa tcttttccaa gtcctagttg aaaacctgta ttatcgccac
    25021 aagcatataa attaccatct gattttaaac caaaggtact attacttata ccagtaaaaa
    25081 attctacaaa atcataatca ttatatcttt tatcaaaatc atcaatcaat atgttcttat
    25141 cattaaaata agcaccacgc tgtaaaagtt tattttgctt cattattagt actaccccct
    25201 ttatttatct tatttcatac gaatacttat ttagagttat atctttttgt tcttttaaat
    25261 aagtgttgct agattttgtc tcaacaaagt tattatccat atcatgtata tatctaactc
    25321 tatttgcact aaatggtaat actacatctc taaaaatcac attaccaatt tcacctgtta
    25381 tacattttct atttaaatct aacttaagcc cttctatagc tactatgtta tttccatttg
    25441 ctaatgtatc ataccaataa ccagcctcat ctacagttac ttctgactct aattctaggt
    25501 atattaatat atctagcact ttttcagata atttgttata gctatttttt aatactgata
    25561 tatcagtttg tatttgacca cttataacat catcaaaaga tatattttct atttttttaa
    25621 ctacctcatt tatagctcct gataaatctt tagaatctgt agttaatata tctgtatctc
    25681 caatttttac ttttatttct tttatatttt tatcaaactt atcatctaaa tcttgaatat
    25741 ccttttgagt tacaaaaacc aacgtcgggt ctacttctaa tgtaacattt gaagtattgg
    25801 ataattgtaa aatcattttt acaaccaaat ctttaatact gccatcttca gcacgtggct
    25861 tatacgtctc tggatactta cctatagcta ttatattatt ctcattatcc agaacagcag
    25921 cttctcttat cataaatcca ccaactgttc ctggtaaaat agtctctata actatccaat
    25981 taggattttt ttcatcaatt cttagagaat ttatatttcc ttcccaaact acattcttta
    26041 aagcagtttg ttcctctgtt ggctcataat attctccatt actatctcca acttgaaact
    26101 tggttaatcc aacaagctct cctagtgcag ttgcatttgc tattttttct ctaccaattt
    26161 tggttaatat tatataaaat ttttcttcca attataccac ctccgattta ggataaacta
    26221 ctacattttc tacacctgta tcatttgcaa tagctatatt aatcttacaa ttattttcaa
    26281 tgcatttaat gcttaaagga taaaccttta tttcttctct acttaacatt ccagctccac
    26341 agtttacaac agcactacat tttataattg gttcaaagta tggatgaact tttacctctt
    26401 cactacatac catacctcca ccacaataga ccatactttt atctagtatt attggttcta
    26461 aatagtgtaa taagtgagca ggttttactc tttcaatcat atcactacaa tctaaaagag
    26521 ttttatagtc acagttattt gctataaaac tcaatacgaa tgtaaattca tcactataaa
    26581 cttttatatc tgtttctgat tttgtatatg cctcacatat actttttata acttcaatag
    26641 tactagtacc tctgcttctc attttagctt ttatattgct acgtcttgtg tcaaaatcaa
    26701 gttctttttt ttcaatgcag agtatgtctt cccacatatc taatccccat gttgcagtgt
    26761 caacaaataa ttggttaaag gtatcatcaa tatcttcttt aagaatattt aattctgtat
    26821 cgtatgcacc ttgtatctcc tctacaatgt aatttctatc aaatgatggt agtttatcaa
    26881 ttagcttcat tgattttcca cctcactaaa attaacagtt gttacactag gtattttatc
    26941 ttcattaata gttatattat ctgtacttcc atttataagt aggttactta aatcgtgtac
    27001 accagtagta tttataagta ttcccattac ttttgtatag attatctctc ctctaatatc
    27061 tctaaagtat gtatttatac tttctaggaa agattctttg acattgtcta atgtatatcc
    27121 atcttctagt ttcattactg cacttatact tatttctatt ggtaatggtg tcacaactgt
    27181 tatagttggt ccaataggct tctcttcatc tatatgttgc tgacaccttt caatagtttc
    27241 tgtatcaaca gcttgattat ttttcccaaa tatcaagacc ttaactgttc ccggaccatc
    27301 ccatcttgga taaaccttaa cattatagac tccatctact tccaaagccc attcttcata
    27361 gtgagcttta tttccacttg tagcttgatt tctttggatt ttataaaatc tttcttttag
    27421 ctcttcatct gtctctatat ctgtaccacc ttcaaaactt cttgtgttag taatctttgt
    27481 tactccagat atattatcaa ctagtttaaa ttcacaattt gtaggtaaat tatatttctt
    27541 accaacttcc agagcttgaa ctgggctatt gtcaccttct tcactaccaa tagttacatc
    27601 ttttattact acaaatagta aatctctata tgatattatt gtgccattat ttattacagt
    27661 acctttctct ccaataaatt caacttctcc atttgactct gtacctaact ttctatatac
    27721 accaaattca ttgactcttt tatcaagaaa ctggttatat gtgtcttgta taaaagccat
    27781 tttatgcatc tttgaaagtt ctagatatat cttcgaaagt tctagattat ttccagatac
    27841 catgttgttt agaaaagaac cttctccttt ataaatatca agattaatat tttcaagagt
    27901 tctatttttt attacttcat atgtctggtc actatacatt tatttccacc tccccataga
    27961 tagtggatac ttttacattt gcactcaata tgtctctgtt aaagttagca ctttttacgt
    28021 ttacttctaa tatatatgga tttattagaa gggcctcttt tatgaatcta ctagcttcac
    28081 tttctgtaag accttttgta tatttttgtc ctattagttc tgaaagctct gtcccatatt
    28141 ctaaagagta tatctcatgt tcatatctat ttgtctttat acacttgtat atccaaactt
    28201 ttaaggcttc ttttttttca attattttaa agtcaccttt ttctaaaata ggttcatctt
    28261 tttcaaaatc ccatgccact tcacgaaaga ttggcaattc ttctgtttta ggtaagatat
    28321 aatcctctgg gacacctata aaaggaaata ttgtactcat tataagctca ccaacttact
    28381 tacaacagca aatttttcgc ctattctaaa cattattacc atatccccag attcaaagtt
    28441 atctataaaa ggatttttta cttcatgctg gtgttcttga tttgtttctg tgtcaaatgt
    28501 ttctagctgt ctatcaatca tccaactatc tatcaaaata tcttcttttt ctaatatgat
    28561 gttatttgtc tctattttta aatctggtaa tttactttta atttttccaa taaaaaaaga
    28621 aggttgatta tgatactttc cttcttctct tattattcct ataaattcat ttattggatt
    28681 agccattata tcaccatcct tttataatac tctcctagct gtgttaaaat ctcttctact
    28741 acttaactta cttattttaa ctacatcacc tttttgtgga gcatgtaaaa attctccatt
    28801 acctatatat aatccaacat ggctgactgg attatgaaaa aatactaaat ctcctgcttg
    28861 aagatttttt tgttctactt tcttgccttt tttagattgt tgatttgacg ttcttggcaa
    28921 actaacatta acttttttaa aacaatagac cattaaacca gaacagtcaa aactacttgg
    28981 tccatttcca ccccatttat aaggtttacc tagatgtttt tttgcttctg atattactat
    29041 tttagcttta tcagatacat taccagaata accaccaata ataatttttc cttttcttct
    29101 tccaaattta ctagcttctt caacatttcc aaataaaata tctatatggt atgttccatt
    29161 cttttctata gttattgcag gtcctacgtc atttacttta tagactccat cacgaccaga
    29221 aactcctgta attacctgta tttcatctcc atagtttaat aaaggatgtt tatttaaaaa
    29281 ctcttttgta taataagttt gctcatattt accaaccata ggagcagcgc aagttttttt
    29341 agatgggtca agttttttct ttctacaatc tgtatctcca ccttcttctt ttctaggaca
    29401 gtaagctgta aattctgctg taaactcttt tcctcctgca taatcttctc cccctaaatt
    29461 actttcttcc ttaggttcat cctgtcctgc tgacttttca tccataagat tttgaaaatt
    29521 aagttcaagc tcaatttgat attctccatt ttgccaagta tgtttgtctg tatctatata
    29581 aaatagtcct acaagctttg tataagaatc tttaactttt actcctctac cagttataca
    29641 acttacatct ccataacctt taagagaaca gcttttttct atcccattaa actcgctatc
    29701 aatatctaca tcttgatttt cttgttgctg aattactttt tgcattatta catttacttc
    29761 cttaaaaatt tcattatcta ttttttcgct aatcttgctt ccatactggt ctaccactat
    29821 tactttattt tttacatttt ccatgctctc cgaaaaggtg gtatttataa tattaaatcc
    29881 ctcttcaaac ataacactta atgtaacagt tcctttttca ataacattaa acttatctaa
    29941 attggcctct atcatatact ttttctttgt ctttttactt gcttctgtat atgcactcat
    30001 tattgtgtca taaccattta ctcctataaa catctttgta tacttgacat tggtctttgc
    30061 tattgttcca actgaaagcc tattttcagc aaatacttgc tttgctatgt cttcaactaa
    30121 cttatcttta aaattgtatg acacttcact ttgtgtaagt aaaaatccca tatctttaga
    30181 tacaaaacta atttcattac tgcttgaatc tttagaccta ttaattatca ttcctctaaa
    30241 gagttctttc tcatctacat agaaacagac tgtactagct attggtatat cgatttgttg
    30301 gaaatttaca tcaaatgatg attgtattat tgaaaactct agtgtccttg atggagattt
    30361 ataatcacct gaccatgata ccttgtctac tatgtcagtt atgtcatata tacttccgtt
    30421 ttttatgtgt acccaaatct taataataaa aaatcacctg ccttttcatt taattctatc
    30481 agcagttatt tataacttat tatctataat agttattgtt ttggagttac ttttggtatc
    30541 tttattcttt tatactcttt taaatctaat gtaaaatata catctcgagt cccatctctt
    30601 tcttcataac taaacccttc aattatgact tccatattta tatttgtttc cgtaattgta
    30661 aatcttaata taaaaccttc cttcatccat ttttctatct tatttacaca atcatatggt
    30721 gatggaaaac ctgtatagtc acagaaagta tagtctttat ttggaaaaaa acttgatatt
    30781 gaaatgcttt taagagctac accaccaaaa gttgctattt cacctatttt gagtatacta
    30841 gaagagttta cagcagcttt tccattaata ctaaaggaag atggaaatac tggaaatcta
    30901 aatctatctt cagcttgtct aagccacatt tccattataa gacaccccct aactgtgcca
    30961 attggatact tgcaactaat tttgatgtta tcttttctat atcagcttct tctctaatta
    31021 ctattgtatc agctaactta gctatgttta tatttccacc attttttctg cttctatatt
    31081 gatttgcttc ttgtttagtt aaaaccattt caccttcatg gagtctggtt tgatagtcat
    31141 tataaggaac atagttcaat ccaaatgcat tattaccttt cttacctgaa taagctttac
    31201 cagaatcacc agcctttgtt ccagcatatg gattatcttt gtaatttttt ttactctttt
    31261 ccactagatt taaaactgtt cctgtgatgc ttccattttt tatcttatta atcatagttt
    31321 gccaccactt gcatatatct ttaactattt tacatacttt atctaatatg ttaaaaaaag
    31381 ctcccaatat tggctctata attttccatc cagtttccaa cagaggtcct aaggtctccc
    31441 atacagtttt ccaaataaca cccagtgtct ctataaaatt gtttatttga ggtgcatatt
    31501 cacctaaaaa agtaaaaatt cttgtgataa taggactaac agcttcaact acaggtccga
    31561 ttagttggaa tgctgtagtt actattgtac caaatatact tattaatcct tgtgcaattg
    31621 gtgatttatt gtttatacta tctagtaaat tatttactaa gtttactact ggaagtaatg
    31681 aatctattaa tgctgtaaaa tcgatatttt gtaatagtgt acttcctgcg ttaacaattg
    31741 cttttggtgc attccctatc attgttgctg catgattttc aaaacgttct tgcttatttt
    31801 caaccatcat tttacgcttt tcagcctcat tagaaattcg atattgagta gaatcagaaa
    31861 actcttttcc attttttaca cttctagatt gcatctggtc atatttgttg ccaataatta
    31921 tcttaccatt cattacattg cctattttac cagccatttt ttcagataag ccagctaaat
    31981 tgcctaatcc actttctata aaagttttta ttttctttag tccttgacct aaaatatcat
    32041 caaaaggttt aacaatactc ttcaagccat tgttaatgct tgtttgagct cctgatatta
    32101 tacctggtat tgtattactc atttctttag ctaaccccat ctgctttaca cttatgcctt
    32161 ccaatgcttc atcaaaagtt ttataatttt ctcctaccag agtattaaga gattccatgt
    32221 ttccttttgc tgcacttaag aaagctgctg atacatcttg ttcactactt gtattcatat
    32281 ttaaagctct aacatctacc atagcttgag ttatatcttt ttcttttttg ccaccagtaa
    32341 tattactaat cattttagct ttagttccca ttgcaacaac atcttcaggg tcaaaaggcg
    32401 tatcatttgc cattcttact aaactgccat aataatcatc aacatctatt tgattaactt
    32461 ttccagtatt tcttttcatt acactttcta gaacaacttt ctgattttgt tcctgaccaa
    32521 gtaaatcaac accctttttc acagcgacac ctatagtagc accatttgtc aaatcagata
    32581 tcttcccttt tatcgaacca agtattccac ttgccatatc ctttacagct attgcaccac
    32641 tccatacctt acctgcaaat aatctcaatt tgggaaatac tttacttata atgccacttg
    32701 ccatatcttt tatagctatt acaccactcc aaaaagtatt tttaaacaac attaacttag
    32761 gcaaaattct acccatagca ctactcacca tatctttaag attaatactt ccttgataag
    32821 gacgtttctc tacttgtttt aattttccca agatactact tatcactctc ttagtcatat
    32881 cttttgcttt tattacgata ttccaaatct tgccaccaat cttttttaaa tttccaagta
    32941 atttattttt agttttttct acttcatcac ttttagcttt aaatattaat ataatcataa
    33001 tatttgaatt aaactcattt ttcttttgaa tcaacttatt aaaataatca ataatcatag
    33061 ctatagttat tgctagtctc ataatacttc catttaaact tccaataact cctgaaacag
    33121 catttatgct agaacttaat tttgtcatag ttgaacttac actactagca gtagtttgaa
    33181 cttttcttaa cgaggaattt aaactgttta ctctattact tgcacttcca atagaatttt
    33241 ttatgctact agccattaca ttagtggaaa gtgaaacagc cacagttgct ctcctaaaat
    33301 tatcagttac tgatataatt tgattcattt ttggtgtgta attatctgta aggtacatat
    33361 ttataccttc ttctctcaca tttcccatta cataccacct cctgtcaaag gacacactcc
    33421 atatccatgt ttcatttttt caacttcttc ctcaatttct aaggcgaaaa aagcttcaat
    33481 aagctttaat tcgcctttat ccattgcata aaaaagggac ggtcttatac cttttttctt
    33541 ccaataatag tacattattg tagtcctggt atccgtccct attagttttt tacctcttct
    33601 atcactgcat ttttaccata acccataaca tcttgaagta ttctatataa gttggtaatt
    33661 tcacctggta ataacaattt tcttgctaat tcttttggtg ttggagcctt aaatttatcc
    33721 attagctcct tatttttaaa taaaagcttt ccatccaaat cacaaacacc ttctataatt
    33781 gtaagcatct gcatctccgg taagtctata tccgttttca tattttcatc aattttaaga
    33841 caagtatttt gtatctcttc atctttactt attgttaaag ctctacacat tactttaaat
    33901 tcctgcccaa atatagttga taatctctta agttcaacta tagtacttgg tctttctaag
    33961 ttttctgtat ctgcatttaa aagtaaatct actatgttta tattttttaa taatccattt
    34021 tcactcatta ttatttgctc ctttatcaat caaattcaat caactaactt ttttacttat
    34081 taactatctg aatttattaa gtatatattt actaaatatc agtataaaac caaatataaa
    34141 actaaatata aaactaaatt atatcaagaa aatcatactc agtaaatgtg aaaggtgctt
    34201 ctatctctcc ttttacgcca acctcccaat cagctaaagt taaatcatca aatgctatat
    34261 tctttactgc tattctttct gctccataag aatctgggtc atttaattta gatatagcaa
    34321 caaatctagg ttcagaacct ctctttatct tttctccaat taacttgtgc attcttgaac
    34381 taacatggta tagagttatt gagccttttc ctttatatcc catatacttt gtatcagtac
    34441 ccatttgacc tgctattata atatcttctt ttgtaaattc catctttgct tgaaacttct
    34501 ttacttctgc tactttgttt ccatcaagcc aaagttctcc ccaagtacca ctcattacat
    34561 ttctagcttc catatttgcc atattaataa tcctcctata tttctattga taaatctata
    34621 tcttccatag catcaagtac ttttattttt gcttttaaaa atactttaga acctgtgtta
    34681 gcttctttta tttcttgtaa tgtcatataa gataaatcta ctccttttga ttttaaatac
    34741 gatttttgtg cttcaaaatc tatttcaaca gtagaatcag attctataag tgctgatttt
    34801 tctaattctt ctaaataact ttttatagct actatcaata aacatttgtt gtcataactg
    34861 ttagtaacct ttcctatata gtcatctatt atcacctttc ttatgtcact atgtataata
    34921 tctaaagtgt caactatttt tattttctgg aacatttctc ctttttctgc tgttaattca
    34981 gttaaagaat ttactcctct agcaattctt atagctcctg cttctttaat aagtataagc
    35041 tctcctttat taacctttga ttctgcatca actttcgtca tcttaggtat atcgactaca
    35101 tcactaagtt tagtataagt tactgattga cttaaaggtg tacctgctat aagtccagcc
    35161 accctacttg taaactcatc aacactgtat ttcttttctc caactaacac atcttctgta
    35221 gtaaaattaa ttatcccttc atgatttcct acaacttttc ctaatacagc tttaacctta
    35281 accttatcta tatctctaag tttaattatc caatttttta tagcagtctt atcagcttct
    35341 actgcttttg gcatacatag ataattaaat tccttagtct ctaaaaaatc taatgcagtt
    35401 tgaatatctg cttctccttc tattacataa actaataatt tatttggagt gttaacattt
    35461 cccatcaagg ctaaatttat atattcttta ttttcagcag ataaatcaac tggtatatcc
    35521 tctttttcat gtatttcatt aagacctagt gccttagcat ctttaagcac cattgcaatt
    35581 attcctctag ctgaacgttc tttaacagtt gtagctagct ccttaaatga tatgttgata
    35641 cttggtaatc ctatagccat actatcactc tcctaaattt aaattgattt cttccatatt
    35701 ttcatatact acatcttctt catatacact atccagatag tttagtgtaa gtgtaaagtt
    35761 caatacataa attgactctt cttttttaat actttgttct agctttgtta tatttaattt
    35821 tctgtctgtt acctttatat ttctgttaaa tagcttattt aattcatctg aaacttcata
    35881 cattctaata ctctttgact gtggttttgg taagtatttt atagagataa caatatcttt
    35941 gttattcgta tttaacatta caggtgtaca aataactgat agtatactta caaaaaaaca
    36001 agacccttca gagccttgca cactttcatc atcaataaaa atactttcag taaagttttt
    36061 tttgagaatc tcaataattg tttctaatat ttccttgtat ttaagcaaat aatcaccaca
    36121 ctatcactaa atattcttgc ttaatctgat aatcttacta taaatgatat aagtcatttt
    36181 taaatatata tctcatttat tcctgtcaag tatcttctat atttttaggt aatattattc
    36241 ttttcaatct atcacctcct tcaatatgga tttctttcac aataacatta tctcacatat
    36301 tttattgcct attgttgcaa tggtgtatat aaagtgttgt aaaattgtcc tattttttat
    36361 aatatctcac tcaatttaaa tatcatcttt ttctttaaat tgtagcaaga tgttctatct
    36421 aagtgtagtt tcatagaaat atatgtcata ttgttttttg tcttactatt ataaaaaaga
    36481 ttaaaaaatt ccatttctat atcatttaga cacgttagag cattttctat cttctctttt
    36541 tctatttcta tatcttcttt ttccattttt aattttctta atctttcctc tctttttaac
    36601 acctcatttt ccacagaaga gcttatgtta tatgtgttac tagtcttttc tgtatatacc
    36661 attgctccac atccagagta ttcattctca catcttttta tgtccaagtt aatattcttt
    36721 atctttatat ccaaatactt ataattatgt aatcttttat ctgtttcttt aaataatata
    36781 tcttttttgt tttccatagt acttctcctt ttttatatac ttattattag cttcaaattt
    36841 acaataatct ctttagtgca cagtttttta ctaatctcac aactttcaag aacatattgt
    36901 tctatgactg tataaaaaaa acttcttcta tattttgttc taaaatacta gaaatcttct
    36961 tagccataag aagtccagga ttttttatgc ctctttctat ttgagatata taatcatttg
    37021 taattccagc ctttctggca agttgaattt gtgtcatacc tttgcatttt ctataatatt
    37081 tcaagttatt tttaaacatt gttttatacc tccattacta ttaagaatat tttgttcttc
    37141 ctcacttaga ttatatagaa ttatttgttc tttatcaata tattttagaa aaaaatattc
    37201 ttttatattt tattagtttt attttttaga acaatttgtt ataatctata cataaggtgg
    37261 tgttataatg tttgccaaaa gattaagaga acttagaaag gaatttggat tgactcaaag
    37321 agaacttgga gaaaaagtag gcgtttccca aagagtctta gggtactatg aaacagaaaa
    37381 cagatttcct gatgaacaca ttttaaataa attggctgat gtatttaatg tatcagttga
    37441 ttaccttctt ggaagaacct tagttaagga aaatattgat acagtagctg cacatagaaa
    37501 aaatccacat gaagagttac ctgaagaagc acaagaacaa cttaatgatt atatagaatt
    37561 tttactaaat aaatataaaa aaaaataaac catatgatga gcagtctatt tttctgctct
    37621 tttaatataa tttaaaggca aacatacatt ctattttagg gggattttta tgaataattt
    37681 ggataagctt tttgaattag cttctcaaga agggataata attcattata ccacttatat
    37741 tgcaggtgat ttagaaggac tatatataaa caaacatggt attaaaatta tatcattact
    37801 cagtaattta aaacaaaact caaaaaaatt aacatccatc ttagctgaag aacttggaca
    37861 tcatttcact agccttggat actatgtatc ctcttataac gattattata caaaaattat
    37921 tatagataag tgcgaaaata aagccttaaa atgggcttgt gagttcttaa ttactgaaga
    37981 agatataata aacataatta actctggcat cacctgcgtt tatgaaatgg cagacatact
    38041 taatgtggat attactttct ttcaaaaaag attagaattt ttatcattaa aaaaacagtc
    38101 tttacaactt ggaaataata aatatttaat attaactaat ttgccgtatt tctacatatt
    38161 tgatccaatt tcataataaa aatagtagta attttaactg gcttaacagt aaaatctata
    38221 tatttatttt aacaaatttt ggtttctata aattcaatat taatatataa taatgatatc
    38281 atattattta aaattctaaa aacaaatata tgcttttgtt aagaaaggtt gtttgatgaa
    38341 tgtataaaga aaagttgttt gataattatt ttaagttctt agcactatta ttttggccaa
    38401 ttatgtggta taagtggata gtaatttcaa atggtactct tgaaaatatg ttatttacca
    38461 tatatgcaat cgtcgcaatt gttttcatca ttttatattc ggtgtttatg ataaaatata
    38521 aagacattac acaaatagat ttcttttaca ggataagtac tctacttgct tttatattta
    38581 cactttttag ctttttaata tacccaaaga gtttattttt cttgtattta aagattattt
    38641 ttactggaat atacctttat tattcaatag taaaaactct taaatttaaa gatgacgaag
    38701 gtgtagtggg tattatgagc tcacttttat taattgtaat aactttgttc tactaaattt
    38761 aattctattt gacttaatat ggctttaaat attagacatc tacattattt tctgatatta
    38821 tttaagatgg tagtcttata ttaataatga aatacactat gaaggagtta actattatgg
    38881 aaaaaaaagt tttagcaaca gttggagaaa aagagataac taatattgat attgaaaatg
    38941 cattaaaatc attagaccct tatcaagcta tgcaatttaa aactgaagaa ggaaaaaaac
    39001 acttattaaa tgatttagtg aatcaagaat tattcttctt agatgctaaa gaagaaaaat
    39061 tagatgaaga agaaatattt aaattagaaa tgaaaaagat agaagaaaat gtattaaagc
    39121 aatttgctat aaataaagtt ttatctagtg ttaatgtaac agaagatgaa aaagtaaaat
    39181 tctttgaagc taataagagc tctttctcta aaccagaaag tgcaactgct aaacatatat
    39241 tagtagatag tgatgaaaaa gctaaagaaa tacttgctca aataaaatca gaagaaatat
    39301 cttttgaaga tgcagctctt aaacattctt cttgcccatc taaagatatg ggtggtgatt
    39361 taggaacatt cggtagagga caaatggttc ctgaatttga agaagctgtg ttctctatgg
    39421 ctaaaggtga agtaagtgaa ccagttaaaa ctcaatttgg ataccatata ataaaattag
    39481 aagaccttca agaaagtaca gagtctacat ttgatgaagt taaggctgaa gttgaaaaaa
    39541 gtttattata tcaaaaacaa aatgaagttt atggaaataa gataaatgct cttaatgcta
    39601 agtacggaaa ccttgtaaaa tacaacgatt aatcataagg tcatatataa aaaagctgtc
    39661 ttttcacaat atttattaga aaagacagct ttttatatta aagtttaaat attcagtttc
    39721 atattatcat tcttttattg taaagaactc agcttaaaag tttaaataag atttttaatt
    39781 atcattagct aaatttttat atccacgtat tatctcataa cagtaaccta ttacatctct
    39841 tctttttatt atcccaataa atgtgtcttg gtcatctata acaggaatga aattttgatt
    39901 taaagatttt ataactaaat cttcaatatc tgcgttgatg gaaacaggag aattatccat
    39961 ttttctattt atatctgtca ctggaacatc ttccaatccc tttaagtcca gatttaaatc
    40021 attttttaat gtccataaaa aatcaccttc tgttatagtt ccaacatatt taccatcctt
    40081 acttattatt ggaattgcag agtatttgtg atattccatt ttttctaggg cttgccttat
    40141 agtgtagtct tcatatatat atgctacttc actttttggt gttagaaaga ataatatgtt
    40201 cattacacta cccaccgtta ctgaaaactt ataaatatta ctcaatattt ataatgttac
    40261 ttttttgcag taactttctc ctttcttatt ttctaagtat tcttaatact atacgctgta
    40321 acaagcgttt ttcctttgct cattttaatc ttattatgct ttattaatat ttttatatgg
    40381 tctatcagta tcacttattc tctttttaga aaatttacta aaaattaatt gacttaaact
    40441 tagccataat aattttactc tacactatta attttaacat atttatctca ttattaatga
    40501 ttttatttta ataattttaa tttattttgc tattaatttt atttacaaaa actgaaaaaa
    40561 ccgctaaatg attagcggct tatctctcat attactagaa cactaatata tatgaatttt
    40621 taattataat ctaactacgt tagcagcttg aggaccttta gctccatcta ctacttcgaa
    40681 ctcaacgctt tgtccttctt ctaatgtttt gtatccatct ccagttatag ctgagaaatg
    40741 tacgaaaaca tctccttcac cttctacaga tatgaatcca aatccttttt cgttgttaaa
    40801 ccatttaact acaccagttt tcattaataa atcctcctaa aaaaacacaa atatttagct
    40861 actcttttct gtaaccttat tactactcta tcatatatag ctatgtttgt caattattat
    40921 tatttttata cattaataat caattttttt actttataat tttaaatgat tattatcttt
    40981 tacactttat ttttctttat aattttttaa taatttattt ttattatatg gaatcttatg
    41041 gacttatcac tccagcctaa atcccatggc accaacattc tatcagtatt atggcaagtt
    41101 accaaaggat accctcttga gtctaatcct gtcactgtcg aaacatgtgt tattctgcct
    41161 ttcttctcat aaccaacaaa atcccctgga cgtaaattat aagcttcttt gtatacttct
    41221 tcataggaac ctttagcaat taatgaacct cttccactat acagtgcata atttttaaaa
    41281 gcttgtgcat ttacccatgc ttttgtacct gctctctcgc tataattcca aattgagttc
    41341 tttttgaacc ttccactttc aaacattatc tgtgatgcaa agtttgcaca atctccacct
    41401 tctggattat aatccctata gtttggatta aatttcatac catgttcttc atcagctgcg
    41461 gctccacagt atttatgtgc ataatctata gccttttttt gttcatctgt aagctgtaac
    41521 tctacaccat cttgttgacc aatatagttt cttatatcat cagattttat attttctaaa
    41581 tttaaagagt ctgcaaaagg gtctgtatac cactctttag ttatgacata ttgattatct
    41641 ataattttta catttagata atgatatgta cctattctaa aggcattttc tttgtccttt
    41701 tgattttcat atgaatactt atattcagtc gatactgaac agattatacc atagaggtct
    41761 ttttcttttt cttttatttt tttaatctta acccttgtct ttatgtcttt aaattttaca
    41821 ccttgcttac tagaccagtt ttctaaatac ttcatctttt ttacttcatt ttcataagcc
    41881 cacagaccaa attttttatc cgtctcataa agacctttta aaatgttttc atttttctct
    41941 aatattgcct tatttctgta gtcaaataag ttttcaagca atagtttata ttggtctttt
    42001 acatcatcac cttcaacatt tgcagattcc atgacttttt cttccatatt tttcatactt
    42061 aaatcattga ttaaaaatac acctgctgta gtagctaaag acaaaaatac aaatgccatt
    42121 atacctttga tgtacttatt cttatttttc agtattttca aagctacacc tcccaaatag
    42181 tacaccttac aaatcaaatt acaaagaata ttctgtcaca tatagtaatt ttcatattta
    42241 ataattctct attttattat gtccaatatt aaaaattaat tgtatcaata cactgttttt
    42301 tttacaattt tttattttat aatctacttt tttctttttt ttatagtaat atatatgtat
    42361 ataaatattt tggtatcatt ataattaata catactaatg gagggttttt tatgcaaaaa
    42421 aaagtcgcag caattaatga cttatctgga ataggaaaat gctcattatc ggttgctata
    42481 cctatacttt cagcattaaa ggttcaatgt tgtccatttc caacggccat actttcaagt
    42541 caaactggtt atcctgaatt tactttctta gactttactg atgaaatggt aaaatattca
    42601 aatgtttgga aaaatttaaa agtcaatttt gatagtatat atagtggttt tttgggttca
    42661 aagcatcaaa ttgagatagt ggctaatttt ataaacgatt atcccaatgc ttttatcgta
    42721 gttgaccctg tgatgggtga taatggagtt atgtatccaa tattcacaga agaaatgaga
    42781 caggaaatta aagaattagt taaacattca gatttaacta ctcctaactt aacagaggct
    42841 tgtttcttga ctggaaatga ttatactaaa agcgattata atagagatga gcttatatat
    42901 attgcaaaat ctgtatctga tttgggacct agcaaagtcg tgattactgg tatattagaa
    42961 gatgataata tattaaattt ggcttacgat agagataatg accatgtatt ctttacatca
    43021 gtcaaatata ataattgttc ttacagtgga actggagata tattcacatc tatactttgt
    43081 ggtatgttag taaataaaca tgatttggga gtagcggtta atactgcaac agattttata
    43141 tataaaacta taaattacac atctcaattt gatactgata gaaatgatgg agttatgttt
    43201 gaaaactttt tatcagattt aactaatata taaagtcaaa aaaactatcc attttataaa
    43261 tacctataaa tcataaaatt taggtattta caaaatggat attatttact aaactattgg
    43321 tatttcaatt gttaccatag caccaccagt tttcacagaa ttttcaatat ataaaatccc
    43381 attatgtttt tgtacaatat tatttacaat atagagtccc attccataat gagatttaga
    43441 atttctactc aaatctcctg tataaaactg ttctgtagct tttgacaaat ctgctttaga
    43501 aaaaccactt ccacaatctg taactacaaa ttttaagtat ttattacata ctgatacact
    43561 tatgtatagt ttactctggc ttggtgaata atctatagca tttgaaatta tattcataat
    43621 agctctgaat aacagttctc tatctatagt tatattttta ggcaaattat cttgtttata
    43681 aaactccaca tctaagtttt tagtactagc taatgcttct atttgcacta agacatcttt
    43741 tataaactca ctcatactga ttgcttcttt atttaatata tagccattct ctgtttttga
    43801 tatatctatt aaagtattaa tatatttttc tatttgagtg acaccctttg agatatattc
    43861 catgtattct aataactcag gattttcatt tatttcaatt aataagtcag tatttccatg
    43921 tataattgtt agtggtgttt ttatatcatg tgccagtgca gatatctgtt cctttctatt
    43981 ttcttctagc atccattgtt tttctaaaga atcttttaac gccaacttca ttttatccat
    44041 agaaaaaagg acattattaa tttcataaat tttactagat tctacaacaa aatctaaatc
    44101 ctgttgttct attttttctg tagtattttt taaaagctcc atttcaatat taaatttctt
    44161 gccaaatact tttgataata taacaatttc aataaaaaat attatactaa aaataatcat
    44221 acccaatgtc tctggttttg gtaaatatgc tcttaaaatt ggagaactat actctgatac
    44281 tattttgtat tttattataa aaacttcatc tttcttaaaa aactttatat aactttctga
    44341 atatgcatgc ctttcttcaa tatctctcat taaattccat acttcttttg attctttttt
    44401 attaaaggta cctgaaatta aattcccatc taaagtataa accccatact catatatact
    44461 tggaattata tcttcagtta ctttttcact tgaaataatc tgttctttat atttattaaa
    44521 ttgtttttct gcataattag ctggtaaaat tacacctgat gataataata cagaaaatga
    44581 taacattaaa aagagtacaa ttgaaattgt cataataaag aataaagata aatattttat
    44641 aaaaatagtt cttaactttt caactttttt gttgttagat tttttacctc caagcatttg
    44701 taccctgtcc tccaatagtt taattaattc ataattaata cccaaccagt tttttatttg
    44761 attattttat atctttttat tgctaagtta tattttaaca taataatata aataaacagt
    44821 caatttttac tctattcttc tgatttttta ccttcccatt tagaaaacca aatacataaa
    44881 attacaaatg ctaaaattgt tacaaaacca caaattaaac ctgttgattg atatgatttt
    44941 attgattcaa cctttatata ttcaaccgtt ttagaacttg aaaaagacga ccaaatagtt
    45001 ataaatctaa caccccatgc acaaggaaaa tactgccaaa taccagaacc caatccagtt
    45061 aataataatg ctgaaagtaa catttcaact atacctactc caattgatgt tccttttccg
    45121 aaccttaaac ttaagaataa atgtaatatg tattcaaata aattacacgc aattaaaatt
    45181 agaattgaaa tcatataaaa gcttgatgta aagtaagcct tagatatacc aaatttaaaa
    45241 cctaatacag acactaatgt tgctacaaaa ccaagacata ctaagattat atatttactt
    45301 ataagagtaa gatattttgg attagatgtt gttaacatat gtttataatt ccctgcataa
    45361 tattcttgtt caacaaacat tgaacacaca acacctgaca ataaaggaaa agctattgct
    45421 ataacttgta aatatcctac tatctttagt atattatcag ctcctgaaac cttataatac
    45481 cataaaaaca atcccatccc cattaaagca atgcaaatgt gcattagtat tataaaattt
    45541 ctctttaatt ttattaaatc agaacaaaat attcttatta actgtctcat attacttcac
    45601 ctctctatta ttgaaccaaa cccttgtagc taaagcaaat aatacaaata aaacaataga
    45661 tattaatact cctggcaaaa taacagcatt atttaaaaat tcacttccct ctggtacagg
    45721 aagaccattt ggcagaatat gcaatacaac agccattaat cttgaaggaa tcgaatatgg
    45781 aaacatatac cacatttctg tagttgagaa aacagcaact ccaacaatag tgaagaacat
    45841 atttattaga actgtaacaa atataccaaa ttttgaagct aaaaacatac aaagaggaat
    45901 ctgccacata aatagtaaaa taattaatat agttgcaatg atattttgac ctattgaaat
    45961 agtttcttta tagattatcc caacaaaaac tacacctatt gcagaaataa ttgatgctat
    46021 aaaacaacaa ataatacagt aaatcatttt tccagtccaa acgtctttca tttcaattgg
    46081 caagggtaaa ataccacgat atttactttt ttcatctttt aatactatta aagaagatat
    46141 taatgttaat gctcctggaa gaaatgtcgt ataccaccag ttgaaagcag cattttgccc
    46201 tccacctagt ccacacaaaa gtatacaaaa cactggaact atccatatca acttcattcc
    46261 aaaagttcct ttcatctttt gaaattccac tctaataaat ctttccatat tactcccctc
    46321 tctttctatt ttcagctaca actttcataa aaagtttttc taagttttgt tcttttgata
    46381 cttctccttg ataacccatt actccattag aaattattcc tatttcatct acaacttgtt
    46441 caacttcaga caagatatgg cttgataaaa taactgtaat tccttgtttt ggaaaagaac
    46501 gaatcaattc tctaagttct tcaataccaa tagggtcaag accatttgta ggctcatcta
    46561 agattaaaag ttttgggtga tttaataatg caatagcaat accaaggcgt tgcttcatgc
    46621 ctagagagaa ttgacctgaa cgctttttaa aggtatctct taaatctaca gtctctaaaa
    46681 cttcttcaat gcgagagtca ggaagaccaa gtaaagtagt atggactttt aaattttctc
    46741 gtgctgttaa attctcatat atagcaggag actcaataag agaaccaata tttgataaat
    46801 cctttctagt ccatctatgt ccctcaaaca ttatttttcc tgaagttgga tttaaaatcc
    46861 cagttatcat ttttaataaa gtagatttcc cagctccatt tggtcctaat aatccatata
    46921 tagaattaga tttaattgct agtgaaatgt tatcaactgc catctgctcc ttaaattttt
    46981 tagaaactcc ttttacctgc aaaatataat tactcataat tttcatcctt tcttttgtca
    47041 aacttattta caagaatcat tctaaatcat aaatataagg attttataag aattaaaaaa
    47101 caaaaaaaga tgtgttaaaa acacatcttt ttttatctta attctattaa ttttccattg
    47161 tagtatatct ctacttcttc aatccaaatg ctattatctt cttcttggat gacattttta
    47221 ataatattat ttatagtata tattatattt tgttcattca aataatcatc tttccaagaa
    47281 actctacttt ttttatctgg ttctatcaag tttattttaa caagattatc tttttcctta
    47341 acttctactt ccatgtctaa accattaaaa tactcatttg ataatgtatc taagattaat
    47401 cgcagtttat ccttaagtga actttcttca tcaatctgta agtactttac tattttctca
    47461 tcctttgaaa ttatctctat aggtatttta gtagtttttt tagaattaat cattttacta
    47521 gttgtttctt cttttgcatc ttcatccttc tttacaactg ctgattcttt agaatcaact
    47581 agctcaactg cactgatgct ataatccata catgccgtac tcaataaagt agtagcaatt
    47641 attaaaaaac ttactgctat tttattcata agtatccctc tcctaattct ctttcgatac
    47701 ttattatacc atagtataga ttatattttt atacttttaa atcaatttat gaattattat
    47761 agtaaattat ttacaattct aagctatttg aatttatttt tatagtatat tctttctcta
    47821 aattcgacag atttttattc aaaagccctt ttatttccct cgtatttcta tacttgttgc
    47881 tatgttcttc attatgttca taatcatatg aatcattgtc atattcttca tcattattaa
    47941 attcttcaat tgcttctttt gactttatat cacaattaaa tttatactta tttactggta
    48001 tatcaagtct taccttagaa tttggaactt ctatgtccag gaacttagca attggcatat
    48061 cagagtacaa gtaaaattca ctttcataat ttgaatttct acctattttc tcttttatat
    48121 ttacattttc aggtatatac tcttctatat tattgataaa tacatcttca ttctctgaag
    48181 atatatacaa tgactctgat tctatgttta catttttaac gcctaaaatt gattttgttg
    48241 acaagtgaag attgttatta gatattaagt ttaggttttc taatctattt actttgttct
    48301 ctgttatagc tctactaaaa cctccataag aagttttata tgttatagta tttgaagcta
    48361 cattatcgta tacaaataag tgtgagctta tacttgatgc atttatgtct acattatttg
    48421 gtacatatat tataatttct ctataatcat gtgaaaacat actatttata gatgtattta
    48481 ataattggtc gaaattttta atctttttaa ttttattttc atattttctt tcacctttta
    48541 caactaaagt cttatctttt aaagttacat tgtatttgta aaactcatta tttcctcttg
    48601 ttgttacacg cacatcttca cttgaacttt tctttattgt aacgtttata ttgtcaacag
    48661 ctatatctaa cttgtcaata tttacttttt cttgataaag tttattttct tttttaaatt
    48721 cagcttcctt ttctaaacca aagtttatta ggcttggcat aactaaaatc cctgaccatg
    48781 ttgtccctat tatacctatt accaataaaa ctgcagcaaa tatagcaagt tttttattca
    48841 tgtttatctt ctcctttctt attttctata tcttcaaggt ccttatatat cgcctcaaaa
    48901 tttatactat catcattata ttcatcagtt tcttttatgc tttcttcttc tctattgtag
    48961 taatcattat tatatgaatc ttcattaatg tctttattca aatcttcatt aa
//
LOCUS       DBGJFF010000012        48116 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.012, whole genome shotgun sequence.
ACCESSION   DBGJFF010000012 DBGJFF010000000
VERSION     DBGJFF010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 48116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48116
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.012"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(<1..1079)
                     /locus_tag="ACOIAF_000784"
     CDS             complement(<1..1079)
                     /locus_tag="ACOIAF_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HGZ2667810.1"
                     /translation="MKKMLKRLCTGFLALATVVTALPTTPVHAESKQYWTESKERVGI
                     VEKVMNDGSIGSTFNEGHLTVEGEDAYCIDINTDFRNGYKTRADVSTRMSADQISDVA
                     LSIEYVKQYTDSHSGISNNHAYLLRQLVVWQRLSVHLGWQCDNVRASYDEIPKATQDE
                     VFSGAKAFVKENKGRYECGGYIYSGEGQELGQFWAKLNVGNAKLQKTSSNTSITDDNG
                     NYSVAGATYGVFADKDCTKQLATLTTDENGNTDVVEVKAGTVYIKELSAPAGYKVDKT
                     VYSLKVEAGRTATLKVSDTPKVTDTLIKLFKIDMETQKDNPQGNASLAGAEFTWKYYA
                     GFYNKDNLPAEATRTWVTKTIAETD"
     gene            complement(1416..1733)
                     /locus_tag="ACOIAF_000785"
     CDS             complement(1416..1733)
                     /locus_tag="ACOIAF_000785"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGZ2667811.1"
                     /translation="MKNIKVLLACGSGASTGFMATSMRKSAKKKGIEADIKARSESEI
                     DKYLPEIDCLLLGPHLDYMKDEIQKKADEYNVPVACISKTAYGRLDGVKALEEALELI
                     SNK"
     gene            1981..3894
                     /locus_tag="ACOIAF_000786"
     CDS             1981..3894
                     /locus_tag="ACOIAF_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGZ2667812.1"
                     /translation="MKTSKKELFLNFIKTQHDWIDSSTLANYLNVSTRSIRKYVNEIN
                     STGEFILSSKKGYKVNLNNNCQTKVDSSENISPDNRLNLILKELIVNSNGINIFDLSE
                     DLFVSPATIEGDIVKANKFIGSYNLKIKQSKFLLKLIGNESAKRKLMSSIIFKETGSD
                     FLSLFDVQKIYQEYNLTKLKENIIYILKKYNLFINEYAINNILLHLMITIDRIKKNNY
                     IESVEVVNYIDNNNDISISRDISNFLESEYNITLTSAELYYLVFQLTNKTTVLNYHEM
                     DTKSLPNYIDEHFVKLTKKIIKNVYDLYFIDLSDDEFVVKFTLHVKNLISRAKNNQVL
                     RNQIPQKLKDSYPLIYDISVYICNQIQILENIEIDEDEISYISLHVGSFFDRQKLLED
                     KILCALITPNYYDLQFKIVRDLEKRFNESIEIIQIFSDTHNLDFDNKVDMVITTLPIS
                     DRCPIPFVYVNPYLNRKDYDNIQSKFIQIKDRKNILTVQNHLEMYFSESLFMKNIYLD
                     SAKDYIKFMGNILYENKYVKPNYIDDVLIREKMSSTAFNNNVAIPHSMKMDALKTGVC
                     LIVNDKPVKWGEEKVQIIAMIPINEKEKEKFNYIFESFIEILSEWNNVKELTKADNYS
                     SFMNRIAYLIQNI"
     gene            complement(4017..5171)
                     /locus_tag="ACOIAF_000787"
     CDS             complement(4017..5171)
                     /locus_tag="ACOIAF_000787"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HGZ2667813.1"
                     /translation="MKILISIDSLKGSLSSIEAGNAIKKGILKVKEDAQVKILPLADG
                     GEGTVDALVQGMNGKKETIEVTGPIAKKVDATYGLLKNTSTAIIEIAQASGLTLVPTE
                     LRNPLYTTTYGVGEIIKEAINKGYRNFIVGIGGSATNDAGIGMLQALGFEFYDENNKL
                     VGLGGKVLNEIRHIKIENRLKELDECKFKIACDVNNPLFGKNGAAYIYGSQKGATSEI
                     IEELDNGLRNFSKVVKNYLSKDVANVEGAGAAGGLGFAFLAFLNSKLESGIKIILEEI
                     KLEEELKDADFVITGEGRLDNQTAMGKAPIGVAKLAKKYGVKVIGLAGATTEDAVKCN
                     EEGIDAYFSIVNRAMTIEEAMDKATASENMTATTTQIFNLITSIQQSKNM"
     gene            complement(5431..8046)
                     /gene="mngB"
                     /locus_tag="ACOIAF_000788"
     CDS             complement(5431..8046)
                     /gene="mngB"
                     /locus_tag="ACOIAF_000788"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HGZ2667814.1"
                     /translation="MKKNVHIVPHMHWDREWYFSTEESRILLVNNMEEIMDMLENNHN
                     YPYYVMDGQTAILEDYLAVKPECKERIKKLVQEGRLIIGPWYTQTDEMVVGGESILRN
                     LLYGIKDCDEFGEYMKIGYLPDSFGQSAQMPQILNGFDIKYSMFWRGCSERKGTNKTE
                     FNWKSDEGSSVLVQILPLGYAIGKYLPMNEDELRTRMDKYLPVLDKGATTDHIILPNG
                     HDQMPIQKNIFEVIYKLKECYPERKFFLSRYENIFKELEKNEDIDTIKGEFLDGKYMR
                     VHRSIFSTRMDIKSANARIESKITNILEPLASIAYSLGFEYHHGLIELIWKEIMKNHA
                     HDSIGCCCSDKVHHEIMNRFFLAEEKVDQLITFYKRRIVDAMSCEMALDKLVAFNLMP
                     YDRNEIVSAQIITKMKAFEIFDKDNNKLDFEVVHKEVIDAGLIDRQIVHYGNYDSFMV
                     YTINFKDKIPAMGYKAYMIKEVEHMLEKAYEVCDMVDNDFYTIQVNENGTLKIFDKKL
                     NKTFENVLLMENGGDEGDEYDFSPLTNEKLIFNDNIVANSTIKKNRFNNEIKINYRLK
                     VPKDIQARKNNRADSFIDFDIVINVPNDKAIIDVVFNINNQARDCRVRTYIPTNIASK
                     FSVSDNQFGHIKRSVYDDAMEVWQQEEWSERPDSIYPMLTFVGLSDKEHGVAVLTNST
                     REFEIVGEKFDTIAITLFRSVGFLGKEEMARRPGRPSGIKLPTPDSQMIGDIKIDLAI
                     VTHEKSTLEANVANMAKEYLTKIQTYNKMPYNAMKLNQSEIEVDYSYSLLKETNKDLV
                     LSVVKKAEKQEALVVRFYNSKEETKSANIVFNKNIKTAKWINLNEKELESISIDNNSV
                     NVECLMNQVKTILVK"
     gene            complement(8190..10115)
                     /gene="mngA"
                     /locus_tag="ACOIAF_000789"
     CDS             complement(8190..10115)
                     /gene="mngA"
                     /locus_tag="ACOIAF_000789"
                     /EC_number="2.7.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HGZ2667815.1"
                     /translation="MEFKKITNENLVFLKQKIKTKEDAIKFLARKLYDEGKLTSYEEY
                     LEAVMYRETLSPTGFERGLAIPHGKSKSVKEAAFAAMTLETPIENWESVDPNNKVEII
                     FLIAVPENEGGNTHIQILSELVTRLSNEDYMNMLLNSKTKKELFNNLDIKEKKEQIKE
                     EIRIQNESKKVILAITACPAGIAHTYMSAEALIKAGKEIGVDVYVEKQGANGMVDPHT
                     PDIIKRADAIIYATDVAPKNTERFEHLPNIKTSVAAPLKRAKEIIYEALEVAQKQGKG
                     DYIEKSSDISYCEKSSWKKDVKSAILTGVSHIIPLIVAGGMILAFAVLASQAFGLQEL
                     YNQEGSWLWQLRKLSGGMLGTLMVPVLSAYMSYSLAEKPGLGPGFAAGLAADLIGSGF
                     LGGMLGGLLAGYIVKFLKKNIPAKGTLAGFVSFWVYPVVGSLVVGLLMLFVVGKPVAA
                     LNNGLINWLNGMTGVNAILLGAIIGAMVSFDLGGPINKAAYTFAIGAIASGNFIPYAI
                     FASVKMVSAFGVTIATVLGRDLFSESEKEIGKQTWILGLAGITEGAIPFMIEDPIRVI
                     PSLVAGSAITGSIVAFFNIGLQVPGAGIFSLALLEGSMSGFIIASIWFFAAVLGAIIS
                     AILLIITRKQKNQRKIH"
     gene            complement(10245..10970)
                     /locus_tag="ACOIAF_000790"
     CDS             complement(10245..10970)
                     /locus_tag="ACOIAF_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HGZ2667816.1"
                     /translation="MEFYERAKKYEYKFTDTEDMIIEYMRKNKEEIVKLSIQSLADKF
                     FTVPNTIVRLCKKMEYDGFSQLKNNLKEELKNENKEPKNGFESDIKKTLELIDYEKIN
                     KVADIIQKSRNIIVYGIGQNIPLCEILVKEIRGSHNNITYYSQRHEVLHDIGKLGEQD
                     MFFALSLSGETKEVIEAAKIAVDKGCKVVALTHICENQLQQIADISLYCYSTIKQVNG
                     YNISDKVPIMMIMRAIIEQYWLR"
     gene            complement(11233..12003)
                     /locus_tag="ACOIAF_000791"
     CDS             complement(11233..12003)
                     /locus_tag="ACOIAF_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HGZ2667817.1"
                     /translation="MKKLVVRADDIGYSEGVTLGIISSYKNGIVTSAGIMVNMPFSRQ
                     AIEMVKNDDVCFGLHVNLVIGEPCCKEHIKIKNLLDEGGKFVSSKIRRTQLLTGKEVF
                     NYEEVYEEVCAQIETYKLYMSKLPEYIDIHGIEEDVSISAMCDAAKEYGIPSCPYYLD
                     SCISMPSKYPQNYFYKLNKSFVKLFEEKYINLDKDITLMVTHPGFIDYSLIKSSSLIQ
                     ERVYEHAMLCDKEVKMWLKRNNIELVDFKDIFKSNNYI"
     gene            complement(12005..13453)
                     /locus_tag="ACOIAF_000792"
     CDS             complement(12005..13453)
                     /locus_tag="ACOIAF_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HGZ2667818.1"
                     /translation="MKRFLNKMMQQLSLLGKTMLVPIAVMPAAGILGLLFGPNMLNVP
                     AISNISNIVFSNVDYLFLLGAASAYSKSKDKTSVMVAAVVAYMIFKSNLELLNDTLNA
                     GVFGGIIVGACIAWTYNKTYKVKVPAFLSFFAGEKCVITLSPVVALILSYIFSLLWIY
                     PQAAMNQFGFWLGGAGAIGIFLFLFCNRALIPTGLHQVLNAYILFEMGEYTTSTGEVI
                     RGEIPRFMAGDPNAGFFWTGFYIIMMFSIPAISYAIYKTAQDKKKQEVKGAMTAGALT
                     SFMATVTEPIEFSFLFASPKLYIIHSFYSGLGGVVLYLLGARLGSFNGASIIDYVLSF
                     SYGDKAWLVIPVGVVFFVLYYVTFKYIIIKDNVQTPGREVEIEIGDKVSEKEKNLKLS
                     HGNFSYMAKQIIKNCGGYENIVTLNNCMTRLRLEVKDATILNDDNIKKTGAKGVIKLS
                     NTSVQIIIGTDVVKVKDEMEMQLDELRKQADA"
     gene            13823..14545
                     /locus_tag="ACOIAF_000793"
     CDS             13823..14545
                     /locus_tag="ACOIAF_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGZ2667819.1"
                     /translation="MAKNKFSEIYEVLKEEILDGKYTSNMMLPTELQLIERFSCSRNT
                     VRRAISQLNTEGYVQSIKGKGVVVLENSCSNDFFLNMHNFKGVESIVEDKKVNTATSV
                     LHFSKILIDNKLSKKTGFKVGSEVYYLHRLRYIDNIPKILDINYFLCSIVKDLDVSIA
                     QGSIYKYIEECLGTKIVSSRKIFKIEKATELELKTLPLNDYNCVGVIKNSVYTDDGKL
                     FEYTESKHTPETFVFMDVTQRY"
     gene            14742..16409
                     /locus_tag="ACOIAF_000794"
     CDS             14742..16409
                     /locus_tag="ACOIAF_000794"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HGZ2667820.1"
                     /translation="MKNWWKKATVYQIYPKSFKDSNNDGIGDINGIIEKLDYLYYLGV
                     DLLWLTPMYVSPQRDNGYDIEDYYNIDPKYGTMSDFEKLLKEAHKRDIKIMMDMVLNH
                     TSTEHKWFKESKKSKDNPYRDYYFWKDAKPDGSVPNNWISRFSGTAWKYDETTNQYYL
                     HLFEETQADLNWENEKIREECYKILEFWADKGIDGFRLDVVNLLSKTPGLPDDPITGP
                     KGDGRTHYADGPRIHEYLHNMNQKVFKPKNIVTVGEMSSTTPEECINYTRENREELSM
                     VFSFHHMKTDYKGGAKWTNEMFSLDKLKKAQSDFQYKMYEGKGWNALFYSNHDQPRAL
                     SRFGDDRFFRQESAKMLAITLFGLQGTTYIYQGEEIGMTNAYFTKIDEYDDAESKNAY
                     YHMIKSGIDEKEALLILQQKSRDNARTPMQWDNTIYKGFSNHKPWLKVNNDNISVENE
                     LNDSRSVFYTYQRLIKYRKQYDIFTEGTYRLLDENHKNLFVYEREYKNQNLLVISNFT
                     HDNVVYKLPETYLNKLNYTILETNYTRDIIENKMEIKPYESIMIYYK"
     gene            complement(16505..17824)
                     /locus_tag="ACOIAF_000795"
     CDS             complement(16505..17824)
                     /locus_tag="ACOIAF_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HGZ2667821.1"
                     /translation="MEKGIKLGFWSIVLLGINAIIGSGIFGLPGDAYTDIGPASILVL
                     VFCMLLAVSIALCFAEAGSWFDTDGGPYLYAKEAFGDFVGFEVGFMKWIVSMIAWATM
                     ANFFAVTLSSVWPQATEPLIKNIIIGILVVGLGIINFMGMKQSKHLNNIMTIGKLLPI
                     VLFIAVGLFFIKGSNFTPFVIIQKGQSASSAFVAVSITLFYAFTGFESLAVAAKDMEN
                     PKKNVPKALVMVMFVVSVIYMLILGISIGVLGNGLAGSATPVADAAIKMLGPIGGYII
                     TIGTIVSVGGINIASSIFTPRSAAALVEQGLMPKSIRKTNKNGSPYIAIIVSVIGTLL
                     IAWSGSFTTLSQISVVSRFIQYIPTCLAVLILRKKYAGKDVNFRIPGGAIIPIFAVII
                     SILLLIKAGIDEPMKIVWGLGGMIIVVPIYFYMTKVYSKKYNDVEVK"
     gene            complement(17865..18611)
                     /locus_tag="ACOIAF_000796"
     CDS             complement(17865..18611)
                     /locus_tag="ACOIAF_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HGZ2667822.1"
                     /translation="MKKLAKKKYPIIGISGNLLIDEGGMFPGYERAYVNNDYIQSVVM
                     CKAIPYIVPIVYDDEIIKEQVSNIDALILSGGQDVNPLIWKEEPHNKLGAISPKRDSF
                     DMKLLKHALDMKKPVLGICRGEQIINVTEGGSLYQDLSLIEGAYIKHNQQHLSNVPTH
                     TVQIKEGTKLYEILGEKEVLVNSFHHLVVNKVAPGYIVSATSKDGLIEAIEKEGSEFV
                     IGIQWHPEMMTRDYDNMKKIFMAIVKEASK"
     gene            complement(18855..20864)
                     /locus_tag="ACOIAF_000797"
     CDS             complement(18855..20864)
                     /locus_tag="ACOIAF_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HGZ2667823.1"
                     /translation="MKTIAVVTDGLSKLECFLNKNLEMIFKDKVMIKNYYLNKITEKE
                     LIKDDIILVMIDDRVLKIKKYVEDVSKIITINRSIKQKDIYKLFSLPEGIDVLVVNDN
                     KHTVLETISNLYNIGINHLNFIPYNPNEEYRNIKIAITPGESSLVPKYIEEVIDLGHR
                     YIDSSTFIQIIAKLKLDDKEITERLIKYTDEIISLYSGINTKYKELTIKADELNSIIN
                     LSNLGMAMVSDKGNIIICNNSLRDILDIQSNIIGKNINELENENIKKIFSLSKAHDEV
                     IKFNNKYLNVNKYNIESFGRVTGYYFCIQEITYIRKLEQNLSKKLREKGQVARYTFDD
                     IKTVSTKMERTKKLGMKIAESDYTILITGESGTGKELMAQSIHNASPRHSQPFIAINC
                     AAMPENLLESELFGYEEGAFTGALKGGKKGLFEQANNGTIFLDEIGDMPIYLQSKLLR
                     VLQENQVMRVGGENVIDIDVRVIAATNKNLLEMIKGDRFRADLYYRINVLPINIPPLR
                     ERKEDIEVMLKHFMKRKRAISQDVQDIINAYEWPGNIRELKNTAMYINIMSSEDVILI
                     NDLPHNLLNITEDFSKEISILKDRTSIEKVKLVMECLKNANNLNKNIGRNGIVNSINE
                     LGYNITEGEVRGILNMLKELDLIICESGRKGSELNRRGKNILNSI"
     gene            complement(21336..23441)
                     /locus_tag="ACOIAF_000798"
     CDS             complement(21336..23441)
                     /locus_tag="ACOIAF_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HGZ2667824.1"
                     /translation="MALRKDFLWGGATAANQCEGGYKEDGRGLANVDVTPQGKDRFPV
                     LAGKMKMFECDDEHYYPAHEAIDMYHNYKEDIKLFAEMGFKCYRMSIAWTRIFPTGVE
                     DVPNEEGLKFYEDLFDECLKYGIEPVVTICHFDVPMYLVKKYGAWRNRKLVDLYVKYC
                     EVIFTRFKGKVKYWLTFNEINMILHLPFIGAGLYIEEGENEEQIKYQAAHHQLVASAL
                     VTKLAHNINQEFKIGCMLAAANQCEGAWNLDGKGPSCSDMCTGGSQKVSKRITRVIEK
                     GTFYPSHEAIDFYHRYKEDIALFAEMGFKVFRFSIAWTRIFPTGMEKEPNEAGLKFYE
                     NVIDECLKYNIEPLITISHYEIPFAITEKYNGWVSRDVIDLYMNYCETIFKRYKGKVK
                     YWLTFNEINSATMPMGGYLSQGILNEGTTDFINQVDIPQLRFQGLHHQFVASAKAVKL
                     AHEIDSNYQVGCMQIFATMYPYTCNPDDAVKTQRDSRVMNYFCGDVQVRGEYPTYMNR
                     YFGENNIEIKMEKGDLEILKEGCVDFYTFSYYMSTCVSSDSKEDNTSGNILGGVKNPY
                     LKSSEWGWQIDPEGLRYALNEIYDRYRIPMMVVENGLGAYDKKESNGVINDDYRIEYL
                     KAHIEQMKEAVEDGVDLFGYTPWGCIDLVSASTGEMAKRYGFIYVDKYDDGSGDLSRI
                     KKKSFDWYKQVIESNGERL"
     gene            complement(23528..25444)
                     /locus_tag="ACOIAF_000799"
     CDS             complement(23528..25444)
                     /locus_tag="ACOIAF_000799"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HGZ2667825.1"
                     /translation="MRKYEQLSKEIIANVGGKDNISSLSHCITRLRFKLKDESIANDD
                     VLKKMDGIVTVMKSAGQYQVVIGNHVPDVYEVVCDLAGLGGDTIDNSDSDAPKGLLNK
                     FIDIISGVFQPILGVLCAAGMIKGFNAVFTALGLYGETDGAYIMLNAIGDAMFQYMPI
                     ILGYTAAKKFKLKPFIGMLIGAVLCYSGIQLDTLASTGQPLYNLFTGTIFESPVYITF
                     LGLPVIAMNYTSTVVPVILIVLLAAKLEKVFAKIIPDVVKNFFVPMAVLLVSLPLGFM
                     VIGPIATYASNIVGNGFLALFNFSPILCGALTGLLWQVLVIFGLHWGLVPIAMSNLMT
                     MGFDTILVGEFVPSFAQTAVVAAMYFKLKDKKIKELCIPAVISGICGVTEPAIYGITL
                     PRKTPFIYSCVGGAVAGAVMGIMNVKSYVMGGLGIFGIPNYINPTTGDMKGVYATIIA
                     IIVAIVVGFVLTLFFWKDDAIVEISKDDNGQGVIEKNKREVVYSPIKGEVKALEQIQD
                     AAFSTGILGKGVAITPKEGKVVAPFDGTVMTLFPTKHAIGIISDNGCELLIHIGLNTI
                     QLEGKYFKSFVNQGDKIKKGQTLITFDIESISKEGYCLETPVVVTNYSDYVDIVENTQ
                     KHVNNSQELITVLA"
     gene            complement(25518..26378)
                     /gene="licT"
                     /locus_tag="ACOIAF_000800"
     CDS             complement(25518..26378)
                     /gene="licT"
                     /locus_tag="ACOIAF_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HGZ2667826.1"
                     /translation="MIIEKILNNNVIITTDENHKEIVVMGRGLAYKKRTGEHISKDKI
                     DKIFKLSDPNISDKFKELIADIPIRYMELSDEIILYAKEKLGKRLNDSIYISLTDHMY
                     TAIERAREGVSVKNVLLWDIKRFYKSEFKIGLEALDYIEKKFEIRLSEDEAGFIALHI
                     VNAQMDQSIKTIYEITQIIQEISNMVKYHYRIVFDEDSVYYHRFITHLKYFAERIVSN
                     NLHENNEDDLLNVIKVKYKNAYKFIEKLDEFIHKKYNYDLTDEEKLYLTIHVERVVSK
                     SIKSDINPNN"
     gene            complement(26729..27916)
                     /locus_tag="ACOIAF_000801"
     CDS             complement(26729..27916)
                     /locus_tag="ACOIAF_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HGZ2667827.1"
                     /translation="MIESIKKRAYEIENELGKEIEEVCNFIFNNPELGEEEYISSKYL
                     VEKMKEYGFDTVYPYCNMETAFRAELGDNDGPTIAFLAEYDALPGYGENKEPAHACGH
                     NWIAASTLGACIVLSKLKENFKGKIVLIGTPAEETTGGKCDLVKAGAFDDVDVSYQMH
                     IEAFNNINCKALAIDSLEFSFEGVSAHAASHPHMGVNALDAVQLTFAGINALRQHVKS
                     DVRIHGIVSNGGEAPNIVPEKAACKFFVRAAERSYLDEVTKKVINCAKGAELMTGAKL
                     SYRYFENSFDNIINNKVLQKITKNNLIEAGITDILEGKDGPVGSTDIGNVSQVCPTMY
                     TEIALDISPMVYVHEKEFLNYANSEEAYDKLHKAVKAMVGCALEIYLEDGLLDEIKKN
                     HLN"
     gene            complement(27930..29321)
                     /locus_tag="ACOIAF_000802"
     CDS             complement(27930..29321)
                     /locus_tag="ACOIAF_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HGZ2667828.1"
                     /translation="MSSLKKKKFTMPQTLTIIFLLIVLMAILTWIVPSGNFERVDIDG
                     RSVVVAGTYEKAPSNPQGITDVFTAPINGFIDAAEVVGFVLIVGGAFGIVNKTGAIEA
                     VIAHTVNKMKKFQFLIIPISMILFGLGGTTFGMSEETLPFYMIFIPLMTSMGYDSLTA
                     VATVFIGATAGFGAATTNPFSVGIAQALSQIAPGSGIEFRVVMFIIYMAISIGFVMMY
                     ANKVKKDPKKSLVHDISLNQESMVNSDTNIKEFTKREAMVIAIFTIGMAIMIYGVLKL
                     GWYITEIAMIFTAIGIISGIASGLKQDDIVNSFISGAGDLMTAGLCIAFARGIVIIAE
                     NGFIIDTILNSAANLLNGLPKTIFINLTFLIEGLIAFLIPSASGLASLTIPVLAPLGD
                     LVDVSRQMIVTAYQFGIGVTNLITPTSGVLMGALAVANIPWSKWVRFVIPLMVVLTIL
                     VMAFLTIGLYLGF"
     regulatory      complement(29465..29644)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            complement(29916..31568)
                     /locus_tag="ACOIAF_000803"
     CDS             complement(29916..31568)
                     /locus_tag="ACOIAF_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HGZ2667829.1"
                     /translation="MKDKLQVLSGAMMIPVILLVVAGVFIGLGSAFANMENVSALGLS
                     GLIKEGSFINVFFKVINDLGFMVMRYLPIFFVTGIAFGLSKKEKGWGALGGIVLFIGM
                     HTVISTLLAANGINSDTVTAEAFMASGLGEAEAYSRSSLYGSFLGIFSYDCSIFGAII
                     AGFVASAVHNKFVDIELPSALSFFSGPRFSMIMMFIASIVLGTAMYFIWPPVGLALSK
                     LGNWIGSSGLLGTFTFGAMDKALLPFGIHHLIAFPIEYTRVGGTMEVGGAIYEGVNNI
                     RLAQMGDPDTLSYITRNFTTGRLLIHFAILPAAALAMYKLADNANKKKAISILIPAIV
                     TAMLVGVTEPIEYTFLFVAPLLYFVAYVPLAGLTYVFTEMFNVSIMGESFRNMFPNLL
                     QPQKVDALPLLFLIPIFFIVTYIIFTWAIKKFDIKTPGRSSEEVKLYSKKEYRERQSV
                     DAGKTAYNNAVEEEVDDKDTKLVHSIIEGLGGSNNIKNVTNCATRLRVELNSIDGFYE
                     DGFWVNELGASGVVKKKNSVQVIFGPRVITIASKLKAVLGVD"
     gene            complement(31588..32991)
                     /locus_tag="ACOIAF_000804"
     CDS             complement(31588..32991)
                     /locus_tag="ACOIAF_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HGZ2667830.1"
                     /translation="MIVNKELIIDYLKSNQENIINDIRSLVEIPSIRDESTTDINQPF
                     GIEIRNAFDKLIQIAKDKDFVVKDFDGYAIHIEYGEGEEVVGVLNHIDVVPIYNKELW
                     KSKPFKVCQKDNYLYGRGVNDNKGPLIGILYALLFLRELNEKPKRKIRLIVGGAEETT
                     WECMEHYFSVNEQPKFAFSPDGNFPIVNGEKGILYFNLRKKIDKDKFRNHNLVDIKSN
                     KEDGFVCNKIEAVFKTNDKKDLVESLAYYTEIEELEEGKVLVRYTGERALSRNPHRSY
                     NCAFSLAKDLEKIKKLNDKGVIIRDILNSYFTDDNHGKKLGIYKEDVDMGVSTICIMS
                     IFLEKNELNMKIDFRYPKGISWEFITNRINEIGKKENLIVDIYKDLKLLYVKPDSELI
                     NKLSSAYKQGFGKEAELFTKGAASYARVLKNGVAFGPTIEGDNPNSHQANENISIDTL
                     YKAIEVYIYALYSLAFQ"
     gene            complement(33255..33746)
                     /locus_tag="ACOIAF_000805"
     CDS             complement(33255..33746)
                     /locus_tag="ACOIAF_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HGZ2667831.1"
                     /translation="MFNIFKKKENKIEKIYSPFKGNLVNLEDVPDAVFAQKMMGDGVA
                     IIPSEGKLYSPVDGEIVNVFDTKHAIMIKSNDNTNILIHVGLETMSLEGKPFDVKVKA
                     GDKVKAKDLIMEADLDFITSNDLNIITPVVIMDDADSVKRTVKDRIEPGNVDINSIIM
                     EVE"
     gene            complement(33772..34584)
                     /locus_tag="ACOIAF_000806"
     CDS             complement(33772..34584)
                     /locus_tag="ACOIAF_000806"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HGZ2667832.1"
                     /translation="MRYKLICTDMDGTLMGKGFEVSEENIKALKEAMEKGIKVALVTG
                     RPYNAMKYFTSVLGDDIYIISTNGTYFKLLGYEYKKVLSKEALKKIYTIGEKYNLNKH
                     FKGCKIVISNNEIGEEHPYRLINSKNKEEDRIEIIENASCETLLEKADNEILKCILFS
                     ENVDSLREAKEEFKKQEDLEVVSSGKINFEVMSKGTSKGIAVKKFCDILGIDSEEVIC
                     IGDNENDISMIKFAGLGIAMGNATDEVKSMADFVTDTNVNDGVAKALRKILS"
     gene            complement(34597..35748)
                     /locus_tag="ACOIAF_000807"
     CDS             complement(34597..35748)
                     /locus_tag="ACOIAF_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HGZ2667833.1"
                     /translation="MINFYQPSRINFGQGTLSELPRIIGKYGKKCLLVTTPNVEPLDK
                     LYKRVTLNLIEKGIEVVHFDRVEPNPTVELINEGFELAKKESVDVVLAVGGGSSIDTA
                     KVLALTFGLEKIDWDYMFSTYTNPFKIYDKVSEKELPLIAVSTTSGTGSQVTQAAVIS
                     RGSEKNTIFHQNCFSKECIVDPELMLTLPERITASTAFDAFTHAFESYINPNANDFTE
                     IMSEKSMELVINYLPKVMEDKNNIEYRTKLAVADTLGGSSLANAGAAAPHPLSEIIGG
                     VTHIPHGEALAIVFPKFAEKFYKDNIEKFAKVARMLNQELEKVEDDEAAKTLGNEIES
                     FLKRVGLKTKMSEFNVTKEQLEQITSCPVLGFLPFGKKEDLVEILEKSY"
     gene            36018..36659
                     /locus_tag="ACOIAF_000808"
     CDS             36018..36659
                     /locus_tag="ACOIAF_000808"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HGZ2667834.1"
                     /translation="MKYLIDSANLDEIQKCLKYGFKGITANPSMYLKEKVNFHEFIET
                     CANLNPEILTAEVIGNNLYELINSCDKILNINKNTIIKINFSEVGLELINILNSKGVK
                     TACTLIFNVNQASLAINAGADYLFPFIGRNDENGNDGIKNLTEICNLISINGYNTKVV
                     AASIKNVFHLTQASLCGCDYAAVTYDLFKKASFNQLTIDGANTFERDWSKVNK"
     gene            36685..38229
                     /locus_tag="ACOIAF_000809"
     CDS             36685..38229
                     /locus_tag="ACOIAF_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HGZ2667835.1"
                     /translation="MNKRLLDIAELLLNSSDYITVDTIAEELKVSNKTIRNDLVILDE
                     WLLEFNLSLDKKTGSGVIILGNEDIKLKVIRDINDKSNCIYAYSPEDRKRYILSKLFI
                     KNSKFRIRDICNELHVSRATVHKDLVVIQNFLQNFKVTLVRKTNNGVYLEGKEKDIRK
                     AIFELATVNKSYTELKEILFSKSNECKDTPSTKIFKELFNYDFEKLSQITLHTLQVEK
                     SNLSDEYYINFLIHIAICIKRISIKRYINLSEGFFRELLEHEYFEKSKILCANLSEAF
                     DVEFIDEEVCYILLHIEGLLKSIKKTTTLEPLKNEEITEYNLSEAIAVHWGSILNLNL
                     KDDLILIKSLANHLKSVLHRINYGFTITNPILNDIKRTFPYTYKAAKESNTVIKQLMN
                     YEVNDDEIGYLALYLISAIDRSKSPLNTILICHCSDGVSNLLVQKLSFEFNQINIVKS
                     IPLSSISFTNFDDVDLILTTAPVDFEHNAELININALLSKNDISRLSTIIKKLYSEKN
                     KILSYK"
     gene            complement(38429..39055)
                     /locus_tag="ACOIAF_000810"
     CDS             complement(38429..39055)
                     /locus_tag="ACOIAF_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HGZ2667836.1"
                     /translation="MKKEMIKDYLLITISAFLMALAVNFFFAEHTLAPGGITGLSVVL
                     STFLSMPVENISLGISMPLLVMGLLFLGKGFGIKTLYITLMGPLFLKVIPQTHITDNL
                     LVAGVIGGLLVGTAIGIAIIRGCSTGGTDLVAMLINKVIKFLKLPVILFMLDGFIIVA
                     SGIISKNFMISIYSLISLLLIIKTIGFVTTKFGKNTEEINNINNACAQ"
     gene            complement(39190..39906)
                     /locus_tag="ACOIAF_000811"
     CDS             complement(39190..39906)
                     /locus_tag="ACOIAF_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HGZ2667837.1"
                     /translation="MKHIKIAAGLAHVNYGHISAVVKEAMDAGVDYVHSDAADMHDLK
                     NMQLMGGHQIIEAIRPVTDKPIECHIYTRDCDRLFIEKIAAAGCNLLIIPAEHFLGAP
                     LAYIINYCREFGMKVGLTIGCYTPLCFVDEAIYDIDRLQIVVHGVDETDGKDNWGWRK
                     SAVDLVKRARKMIDEKNPKCELAIDGGLRADNMEPLIECNPDVVILSSAIFKDKDGIT
                     AGVKNCRKAIDEAATKFGLE"
     gene            40332..41546
                     /locus_tag="ACOIAF_000812"
     CDS             40332..41546
                     /locus_tag="ACOIAF_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HGZ2667838.1"
                     /translation="MRNCIIAQSGGPTAVINASAVGIYEKNLETKYFDKVYFGINGIE
                     GILNKNIIDSDSLNKDNVLNLKQTPSSGLGSCRYKLADYKINSYDYDRVINILDEYEI
                     DTLFYIGGNDSMDTVMKFSQYCKNTTCKIKFIGIPKTIDNDLLGTDNCPGFGSAAKLI
                     STLIMETYLDFSIYKNNGIFIVETMGRDTGWLAASSSLARLNDTPLVDLIYLPEIDFY
                     EDKFIEDVRKIFKEKNKAYIVASEGIRDKYGNFICEQKTSAHDKFGHSQLGGVSNYLK
                     NLIVSSSITTRVKTLELGVIQRCAMHLASDTDLKQAAEVGAYGIELSKKGYTGVMAII
                     NRTCNNPYKYEVSHTDISKIANKTKYFPKEWINEECNYITDEALNYLYPLIQGKPDIK
                     YKNGLPYFSIIY"
     gene            42160..43485
                     /locus_tag="ACOIAF_000813"
     CDS             42160..43485
                     /locus_tag="ACOIAF_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667839.1"
                     /translation="MKIAVITTEFLKEFVDNSIKKLNINAEIEIYIYRDFSHVGDLYL
                     EIEDRFDGFAVSGPIPKKAITKKAGTIKKPLVDFGTDLQSYYDMFLKLIFKYNTLDFS
                     RAYFDLTDWADNPKDISYYLSNGTFGDLMDSIDENASKMSLSDIAKLEKKIAKKHINL
                     WNEGKIDFSTTRFSSIVPMLQNAGVNFHFIYPDIDLLKNTFNNLIKDIHLHRMKGNQP
                     SVIYITVKDTNYDDSTLDQVYKILNSIKKDKLTDFIIEKNSNSFKIFTKCETIKSLTK
                     NFTACYIKESIENSTNIKVCVGYGIGNDIKQAMSSAISANKESKINHLNYSFLVNENY
                     ETIGPLLCDKLLTVSNKVTPHINTIAKSSKLSTLTIQKIISVTEILQRDEVTPKELSS
                     CLNVTVRTANRFLSSLLKSGNATILYEKQNSTKGRPERVYKLFISENKY"
     gene            complement(43613..44803)
                     /locus_tag="ACOIAF_000814"
     CDS             complement(43613..44803)
                     /locus_tag="ACOIAF_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HGZ2667840.1"
                     /translation="MKNFFKEAQSIEGEIIRWRRYLHENPEIGCNLPKTVTFVTSTLE
                     KMGYSPKLTSNNGIVAVLDSENGGKTLLLRADMDALPIMEETDLPFKSKNKYASHSCG
                     HDNHTAMLLGAAKILMDNKHLLKGRVKFVFEPDEETTRGAKSMIDDGILENPQVDAAM
                     AFHSMVGKFLNTGQVAYSRGPAMASADIFEITVEGKATHGASPEFGVDPINIMSHIQI
                     ALNTILSREKPQKEPVVLTIGMFNAGNVPNAIPNKASMSGTLRTFDQFVREKLKNRII
                     EISTGIAKSFGGNAKVEFKMGTPAVINDLDVGDEIVSYVKEVIGNENVIDFPSAMGSE
                     DFSEILLRVPGVFLWIGMGSISQGYNFGMHNPKAIFNEEGLVYGSALFAHCAVKWLEN
                     NFSR"
     gene            complement(44909..46288)
                     /locus_tag="ACOIAF_000815"
     CDS             complement(44909..46288)
                     /locus_tag="ACOIAF_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HGZ2667841.1"
                     /translation="MIGSISINEIDKKRFKLDDLSKKIWENPEKAFKEFKACENTANF
                     LRSEGFDVEVGVGGLATAIRASFGSGKPVIGFMGEFDALPGLNQKVSTKQEAFELGAY
                     GHGCGHNLLCTAHVGAVVGLKREMLENNLSGTIVFYACPGEEQLTGKGFMARGGAFEG
                     LDLAINFHPNKISEATIGTSTAVNSVKFHFKGKTAHAGSDPQNGRSALDAVELTNVGA
                     NYLREHVTSDVRIHYTITDGGVAPNIVPDKASVWYYTRALSREAVESTYERLVKVAKG
                     AAMMTETEVEVEFLGGCYNTLNNHVLANLVSECMNEIKQEPWTREELDFAAELDRQSP
                     QQYKAMISKYNLPEGTHLYYGGGNVTNFNSYGSTDIGDVMHIVPTAYFFTGCTNLGAP
                     GHSWQFTSCAGSSIGEKGMIYASKIMAMFGAKILNNPEIAKKAKEEFDKSMNGKTYKC
                     PIPDDIPTP"
     gene            complement(46319..47527)
                     /locus_tag="ACOIAF_000816"
     CDS             complement(46319..47527)
                     /locus_tag="ACOIAF_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667842.1"
                     /translation="MENFWNPLTCFTVCVMIYAFSEFLSKKTKGAISTLLFVCIIFLL
                     GFWTNILPKDITGASGILAAMSNFGIALLITNLGTLINLEDLCHEWKTVVIALVGIVG
                     IGVGCLTVGSMLFGREYALIAAPPIAGSTVAGIIVTTVAEAANRPELAAFVILVLSFQ
                     KFFGIPITTYCINKELKFKRDNGDFLKDESKSNFKLPSMRIFKEDLGKKSSTLVYLAK
                     LSFVAMIGNFVGLATLIPGFTPANYYLNPNIACLLVGLIFTRIGFLEKGILDKSQSNG
                     IIMFGCMLMLPGGLAKVTPTALFGMIVPTMGILVVGSVFIILFTAIVGKLLGYSPRMS
                     AAIGVTCMLAYPATQIISNEGIDAMEGTEEEKQRATDYLLPKMIIGGFVTVTIASVAF
                     ASIIGPIIFG"
BASE COUNT        15816 a         8491 c         5574 g        18235 t
ORIGIN      
        1 ctgtctgttt cagcgattgt ctttgtaacc catgtacgag tagcttcggc agggagattg
       61 tctttgttat agaagccagc ataatacttc catgtaaatt ctgcacctgc tagagaagcg
      121 tttccctgcg gattgtcttt ctgtgtttcc atatcaatct tgaaaagctt aatcaaagtg
      181 tctgttactt tcggtgtatc tgatactttc aaggtcgctg tccttccagc ttcaaccttt
      241 aaggaatata cagttttatc tactttatat cctgctggtg cagataattc cttgatataa
      301 actgtgcctg cttttacctc tacaacatct gtatttccgt tttcatcagt cgtaagggtg
      361 gcaagctgtt tcgtgcagtc cttatcagca aagacaccgt atgttgcacc agcaacagag
      421 taattcccgt tatcgtctgt aatgctggta ttactggaag tcttttgcag ttttgcattt
      481 ccaacattca gcttcgccca gaattgtcct aattcctgcc cctcgccaga gtagatataa
      541 ccgccacatt catagcgtcc tttattctct ttgacaaagg cttttgcacc agagaaaact
      601 tcgtcctgcg ttgcctttgg aatttcatca taagaagctc gcacattatc acattgccag
      661 ccaagatgta cgctcaatct ctgccagaca acaagctgtc ttaaaaggta ggcgtgattg
      721 ttgcttattc cgctgtggct gtctgtgtac tgttttacat attcgataga taaggcaacg
      781 tcgcttatct ggtcggcact catacgggtg cttacgtcag ctctggtttt gtagccgttt
      841 ctaaaatctg tgttaatgtc gatacagtaa gcgtcctcgc cctcaacggt caaatgcccc
      901 tcgttaaaag tagaaccaat cgaaccgtca ttcattactt tttcaacgat accgacacgc
      961 tcctttgact ccgtccagta ttgcttgctt tctgcatgga cgggtgtcgt cggtaaagca
     1021 gtaacgacag ttgcaagagc taagaagccc gtacacaatc gttttaacat ctttttcata
     1081 aatctaatgc tcctttcatt ttgggtaata aaatagccgt ccaataagaa cggctgaaaa
     1141 tagaaaagga acgtcatttt aggcgttcca tagtctataa aatattcaat ttttttcttg
     1201 tttcaatact gatgtgctgt accatatctt tgcatatcta ggaaaactgg cgtatcaagg
     1261 ctcattcaat ggtagtaaat aagtagtaaa ttgatgtatt tgttcccttg aaaatgctga
     1321 tagatactgt gttttagcgg ataatcagat ttttattgtg tttttcactt aaaaaatcta
     1381 taaatttatt cataaacact ctccccataa atattttatt tgtttgatat taattctaat
     1441 gcttcttcta aagcctttac accatctaat cttccatatg ctgttttaga tatacatgct
     1501 actggaacat tatattcatc agcttttttt tgtatttcat ctttcatata atctaaatgt
     1561 ggtcctaata ataaacaatc tatttctggt aaatatttat ctatctctga ctcacttcta
     1621 gcttttatat ctgcttctat gccttttttc tttgcactct ttctcatact agttgccata
     1681 aaacctgtac ttgctcctga gccacatgcc aacaacactt ttatattctt cataacaata
     1741 cctccagtat ttttatccat taatttattt cttaatgaat ttttatttga tttgattgta
     1801 tttatattgt acttcctaaa taatttcctt tcacataaat atatttccta ataataggaa
     1861 atcatttact cttaaccaga cacataaata tatagtataa tgtacattat aatacgtaca
     1921 ttcaattata taaatatcat atcatacaga ttttatttta aaactatgag agggaatcta
     1981 atgaaaacat ctaaaaaaga gttattttta aacttcataa aaacgcaaca tgattggata
     2041 gattcatcca cattagctaa ttatcttaat gtatcaacac gttcgattcg aaaatatgta
     2101 aatgaaatta attctactgg tgaatttata ttatcctcta aaaaaggata taaagtaaat
     2161 ctcaataata attgtcaaac caaggttgat agttcagaaa atatatcacc tgataacagg
     2221 ttaaatttaa ttttaaaaga acttatagtt aatagtaatg gaattaatat atttgattta
     2281 agtgaagact tgtttgttag tccagctact attgaaggag atatagtcaa agctaataaa
     2341 tttataggaa gttataattt aaagataaag caatcgaaat ttttattgaa actaattgga
     2401 aatgagtctg caaagcgcaa attaatgagc tctataatat ttaaagaaac gggaagtgat
     2461 tttttaagct tatttgatgt acaaaaaata tatcaagagt ataatttgac caaactgaaa
     2521 gaaaatataa tatatatatt aaaaaaatac aatttgttta taaatgagta tgctataaat
     2581 aacatacttc ttcatttaat gataactata gacagaataa aaaagaataa ctacatagaa
     2641 tctgtcgaag tagtaaacta catagataat aataatgata taagcatttc aagagatata
     2701 tcaaattttt tagagtctga atacaatata actttaacta gtgcagaatt atactactta
     2761 gtatttcagt taacaaacaa aaccacagtc ttaaattatc atgaaatgga tactaagtca
     2821 ttgccaaatt atatagatga acattttgtt aaattaacta aaaaaataat taaaaatgta
     2881 tatgacttat actttataga tttatctgat gatgaatttg ttgtaaaatt tactcttcat
     2941 gtaaaaaatt taatcagtag ggctaaaaat aatcaagttt tgagaaatca aatacctcag
     3001 aagttaaaag attcctaccc tctgatatat gatatatctg tttatatttg taatcagatt
     3061 caaattttag aaaacataga aatagatgaa gatgaaatat cgtatatatc tcttcatgta
     3121 ggttcatttt ttgatagaca aaaattacta gaagataaaa ttctctgtgc attaattact
     3181 ccaaattact atgatttaca attcaaaata gtaagagacc ttgagaagag atttaatgaa
     3241 tccatagaaa ttattcagat tttttcagat actcataatc ttgattttga caataaagta
     3301 gacatggtta ttactacttt accaattagc gatagatgcc ctataccttt tgtttatgta
     3361 aatccttacc taaacagaaa ggattatgat aatattcaaa gtaaatttat tcagattaaa
     3421 gatagaaaaa atatacttac tgtacaaaat catttagaaa tgtactttag tgaatctttg
     3481 tttatgaaaa atatatattt agatagtgct aaagattata taaaatttat gggaaatatt
     3541 ctgtacgaaa ataaatatgt caaacccaat tatatagatg atgttcttat aagagaaaaa
     3601 atgtcttcaa ctgcatttaa taacaatgta gcaattcctc atagcatgaa aatggatgct
     3661 ttaaaaactg gagtatgttt aattgttaac gataaaccag ttaaatgggg tgaggaaaaa
     3721 gttcagatta tagctatgat acctataaat gaaaaagaaa aagaaaaatt caattacata
     3781 tttgaaagct ttattgaaat attatctgag tggaataatg ttaaagaact tactaaggca
     3841 gataactata gtagttttat gaatagaata gcttatctta ttcaaaatat ctaaatccat
     3901 taatttattt ccaatatatg tttcttaagt tattaacata aaatagataa gctgtcacac
     3961 taaaaagtct aaattaatat gtgacagctt tttacctaat ataccttgtt ttaatactac
     4021 atattttttg actgttgtat actagttata agattgaata tttgtgttgt tgtagctgtc
     4081 atattctcac tagcagttgc tttatccatt gcttcttcta ttgtcatagc tctatttact
     4141 atagaaaaat aagcatctat accctcttca ttacatttaa ctgcatcttc tgttgtggct
     4201 ccagccaaac ctattacttt taccccatat ttttttgcca gttttgcaac tcctattgga
     4261 gccttgccca tagcagtttg attgtccaat ctaccttcac cagttatgac aaaatctgca
     4321 tcttttaact cttcctctaa tttaatttct tctaatatta tttttattcc agattctaat
     4381 tttgaattta aaaatgctaa aaaagcaaaa cctaatcctc ctgctgctcc tgctccttct
     4441 acattagcta catctttact taagtaattt ttaactactt tggaaaaatt tctaagccca
     4501 ttatctaact cctcaattat ttctgatgtt gcaccttttt gagacccgta tatgtatgca
     4561 gctccatttt tcccaaacag cggattattt acatcacagg ctattttaaa cttacattca
     4621 tctaattcct ttaatctatt ttctattttt atatgtctta tttcatttaa aacttttcca
     4681 cctaatccta ctaacttgtt attttcatca taaaactcaa accctaaggc ttgaagcata
     4741 cctatcccag catcattagt agcacttcca cctatcccaa ctataaaatt tctatagcct
     4801 ttatttattg cttcctttat tatttcccca acaccataag ttgtagtgta taaaggattt
     4861 ctaagttcag tcggaaccaa tgttagtcct gatgcttgag ctatttctat tattgctgta
     4921 cttgtatttt ttaataatcc atatgttgca tccacttttt tagctattgg tccagtaact
     4981 tctatagtct cttttttacc attcatccct tgaactaagg catctactgt cccttcacca
     5041 ccatcagcaa gtggcaatat ctttacttgc gcatcttctt taaccttcaa tatccccttt
     5101 tttattgcat ttcctgcctc tattgaagat aagcttcctt tcaaagaatc tatagaaatt
     5161 aatattttca tacttatcta ctccttttta ttactcctta ttttaaacat ataattttat
     5221 aagcttaatt tatcttttat tttatactct acatctctct ttgtatacaa cttataccat
     5281 taatattctt ttatcttata aaaagggagt gtctcaaaat aaatttctta gttcatgaga
     5341 cactcttttt gtataaaatc aaatatatgt ttattatttc aacaatgata aggaggatta
     5401 tctctatttt aagacaattc ctttttagca ctatttaacc aatatagttt taacttggtt
     5461 cattaagcac tctacattta cagagttatt atctattgat atagattcta attctttttc
     5521 atttaggtta atccatttag ctgttttaat atttttgtta aatactatgt tagctgattt
     5581 tgtttcttct ttagaattat aaaatctaac tactaatgcc tcttgttttt cagccttttt
     5641 tacaacactt aacacaaggt ccttattagt ttccttaagt aaactatatg aataatctac
     5701 ttctatttct gattgattca atttcatagc attatatggc attttattat aagtttgtat
     5761 ttttgttaaa tactctttag ccatatttgc aacatttgct tctagtgtag atttttcatg
     5821 agtaactatt gctaagtcta tttttatatc tcctatcatt tgagaatctg gagtaggcaa
     5881 ctttattcct gatggacgac ctgggcgtct tgccatttct tctttaccta ggaatcctac
     5941 acttctaaat aatgttatag ctattgtgtc aaatttttcc cctactattt caaactctct
     6001 tgtactgttt gtaagaacag ctactccatg ctctttgtca gacaatccaa cgaatgttag
     6061 cattggatat atagaatctg gtctttcact ccattcttcc tgttgccaaa cttccatagc
     6121 atcatcatac acacttctct ttatatgtcc aaactgatta tcagacacag aaaactttga
     6181 agctatattt gttggtatat aagttctaac cctacaatct cttgcttggt tatttatgtt
     6241 gaatactaca tctattatag ctttatcatt tggaacattt ataactatat caaagtcaat
     6301 aaatgaatct gctctattat tttttctagc ttgtatatct tttggtactt ttaatctata
     6361 atttatcttt atctcattat taaatctatt tttctttata gtggaatttg ctactatatt
     6421 atcgttaaat attaatttct catttgtaag tggtgaaaaa tcatactcat ctccttcatc
     6481 tcctccattt tccatcaata atacattttc aaaagtttta tttaattttt tatcaaatat
     6541 ctttaatgtc ccgttttcat taacttgaat agtataaaag tcattatcta ccatgtcaca
     6601 tacttcataa gctttttcta acatatgttc aacttctttt atcatatacg ccttataccc
     6661 cattgcaggt attttatctt taaagtttat tgtatatacc ataaatgaat catagtttcc
     6721 ataatgaaca atttgacggt ctattaatcc tgcatctatt acttctttat gtaccacttc
     6781 aaaatccaac ttattattat ctttatcaaa tatttcaaat gctttcattt tagttataat
     6841 ttgggcgctc actatttcat tcctatcgta aggcattaag ttaaatgcta ctaacttatc
     6901 taaagccatt tcacaactca ttgcatcaac tattcttctt ttgtaaaatg ttattaattg
     6961 gtctactttt tcctcagcta agaaaaatct attcattatt tcatggtgaa ctttatctga
     7021 acaacaacat ccaatgctgt catgagcatg atttttcatt atttctttcc aaattaactc
     7081 tattaatcca tgatgatatt caaatcctag agaatatgca atagaagcta gtggctccaa
     7141 tatgttggtt atctttgatt ctattctagc atttgctgat tttatatcca ttcttgttga
     7201 gaatatactt ctatgaaccc tcatatattt cccatctaaa aattcccctt ttattgtatc
     7261 tatatcctca tttttttcta gctctttaaa tatgttttca tatctactta agaaaaattt
     7321 tctctctgga taacattctt ttaatttata tataacttca aatatattct tttgaattgg
     7381 catttggtca tgtccatttg gaagtattat atggtcagta gttgcaccct tatcaagaac
     7441 cggtaaatat ttatccattc ttgttcttag ctcatcttca ttcattggta gatattttcc
     7501 tattgcataa cctaatggaa gtatttgaac caaaacagaa gacccctcat cactcttcca
     7561 attaaactct gttttattag tcccttttct ttcagaacat cctctccaaa acattgagta
     7621 ttttatatca aacccattta gtatttgtgg catttgagca ctttgaccaa atgaatctgg
     7681 taaatatcct attttcatat attctccaaa ttcatcacaa tcttttattc catatagtaa
     7741 atttctaagt atggattctc caccaactac catttcatca gtttgagtat accaaggacc
     7801 tattattagc cttccttctt gaactaattt ttttattctc tccttacatt caggcttaac
     7861 tgctaaataa tcttctaata tagcagtttg accatccatc acataatatg gatagttatg
     7921 attattttct aacatatcca ttatttcttc catattattt acaagtaata ttctagattc
     7981 ctcagttgag aagtaccatt ctctatccca atgcatatga ggaactatgt gtacattttt
     8041 tttcattgct tatcctccaa aacttatctg ataatatttt ctgtctaatt aaaaatccaa
     8101 taaaaatact actgagttta caaaattttt taatatttat tttaccacta taattaaacc
     8161 tttgatttaa atatctaata acgtatcttt taatgtatct ttctttgatt tttttgtttc
     8221 ctagttatta ttaataatat tgctgatatt atagctccaa gtactgccgc aaagaaccat
     8281 attgatgcta taataaatcc tgacatactt ccctctaaaa gcgccaatga gaatatcccc
     8341 gctccaggca cttgaagacc tatattgaag aatgctacta tagacccagt tatggctgaa
     8401 ccagctacca atgatggtat aacacggatt gggtcctcta tcataaatgg tattgcacct
     8461 tcagttattc cagccaatcc taatatccaa gtttgctttc ctatttcctt ttctgattct
     8521 gaaaataaat ctctgcctaa aacagtagct attgtaactc caaaagctga aaccatctta
     8581 actgatgcaa atattgcata aggtataaaa tttccacttg ctattgctcc tattgcaaat
     8641 gtataggctg ctttatttat tggtccacct aagtcaaatg aaaccatagc tcctattatt
     8701 gcacctagta gtattgcatt tactccagtc atcccattta accaatttat aagaccattg
     8761 ttaagtgctg ctacaggctt tcctactaca aataacatca ataaacctac taccaaagaa
     8821 ccaactactg gatataccca aaaacttacg aatccagcta atgtgccttt ggctggtata
     8881 ttcttcttta aaaattttac tatatagcca gcaagtaaac ctcctaacat acctccaaga
     8941 aatccactac ctattaaatc agctgctaga cctgctgcaa atcctggtcc aagacctggt
     9001 ttttcagcta gtgagtaaga catatatgct gataatactg gaaccatcaa tgtacctaac
     9061 atacctccag aaagctttct taattgccat aaccaagaac cttcttggtt gtatagttct
     9121 tgtagaccaa atgcttgaga tgctaatact gcaaatgcaa gtatcatacc tcctgccaca
     9181 ataagtggta ttatatgaga tactcctgta agtattgctg attttacatc ttttttccat
     9241 gatgattttt cacaatatga tatatcagat gatttttcta tataatcacc tttaccttgt
     9301 ttttgtgcta cttctaaagc ttcgtatatt atttcttttg ctctttttag tggagctgct
     9361 actgacgttt ttatgtttgg caaatgttca aatctttctg tatttttagg tgctacatct
     9421 gttgcatata ttattgcatc tgcccttttt attatatctg gtgtatgagg gtccaccata
     9481 ccattagctc cttgcttttc tacatacaca tccacaccga tttctttacc agctttaatt
     9541 aatgcttctg ctgacatata tgtatgtgct atacctgcag ggcatgctgt tattgctaat
     9601 ataacttttt ttgattcatt ttgaatccta atctcttctt ttatttgttc tttcttttct
     9661 tttatatcta agttattaaa taactctttc ttagtttttg aatttaacaa catattcata
     9721 taatcttcat ttgacagtct agtaactaac tcagaaagta tttgtatgtg tgtatttcca
     9781 ccttcatttt ctggtactgc tattaaaaat attatctcta ctttattatt tgggtctacg
     9841 ctttcccaat tttctattgg tgtctctaag gtcatagctg caaatgcagc ttctttaact
     9901 gacttagatt ttccatgagg aattgcaagc cctctttcaa aacctgttgg agataaagtt
     9961 tctctgtaca ttacagcttc taaatattcc tcataagatg ttaacttgcc ttcatcatat
    10021 agctttcttg ctaagaactt tattgcgtct tcctttgttt ttattttttg ttttaaaaaa
    10081 acaaggtttt catttgtgat tttcttaaat tccaaaatta cgccctcctt aaatcattta
    10141 atgctatgaa tatagcataa aataaattat gaaaatgttt ttttgaaatt atacacacaa
    10201 tttcttctta aaaaataaag atttgtgtat ttatacacaa atctctatct taaccagtat
    10261 tgctctatta ttgctctcat aatcatcata attggaacct tatctgatat attgtaccca
    10321 tttacttgtt taattgttga atagcaatat aaacttatat ctgcaatttg ttgcaattga
    10381 ttttcacata tatgtgttag agctactacc ttacatccct tatcaactgc tatctttgct
    10441 gcttctatca cttctttcgt ttctccactt aaactaaggg caaaaaacat atcttgttca
    10501 cctaattttc ctatatcatg aagtacttca tgtctttgac tatagtaggt tatattatta
    10561 tggctacctc taatttcttt tactaatatc tcacataaag gaatgttttg acctattcca
    10621 taaactataa tattcctaga tttttgaatt atatctgcta ctttatttat tttttcataa
    10681 tctattagct ctaatgtctt ttttatatct gattcaaaac cattttttgg ctctttattc
    10741 tcatttttta actcttcttt taaattattt tttagttgag agaaaccatc atactccatt
    10801 tttttacata gcctaactat ggtatttggg acagtaaaaa atttatctgc taaagattgt
    10861 attgataatt ttactatttc ttctttgttt tttctcatat attcaattat catgtcttct
    10921 gtatctgtaa atttatattc atattttttt gctctttcat aaaattccat agtttctcct
    10981 ccatctattt tattattcat aagtaaaatt caaaaatatt agctaaaatt ttcgttaata
    11041 taattataat ttaatatttt taatttatta atgcatacta ctctttttat tatacataaa
    11101 aaatgtgtac ttcctatatt gatagagatg attcaagttt ttattttgat acatctctat
    11161 caatattttt ttagaaaata cacatttttt tcaaatactt atttttaatt aaaactttag
    11221 gtaatctatt ctttatatat aattattaga cttgaaaata tctttaaaat caaccaattc
    11281 aatattattt ctttttaacc acattttaac ttccttatca catagcattg catgttcata
    11341 tactctctct tgaataagag aagatgattt tataagacta taatctataa atcctggatg
    11401 agtaaccatc aatgttatat ctttatctaa atttatatat ttttcttcaa ataatttaac
    11461 aaaagattta tttaacttgt aaaaataatt ctgaggatat ttacttggca tgctaataca
    11521 tgaatccaaa taatagggac aacttggtat accgtattct tttgcagcat cacacattgc
    11581 tgatattgat acatcttctt ctataccatg tatatcaata tactcaggca atttactcat
    11641 atacaacttg taagtttcaa tttgagcaca aacttcttca tatacttctt cataattaaa
    11701 aacctcttta cctgtcagta gttgcgttct tcttattttg ctagaaacaa attttccacc
    11761 ttcatctaac aaattcttta tcttaatatg ttctttgcaa caaggttctc ctattactaa
    11821 attaacatgt aaaccaaaac atacatcatc attttttacc atttctatag cttgtcttga
    11881 aaaaggcata ttcaccatga ttccagcact tgtaacaata ccgttcttat aagaagatat
    11941 tatccctagt gtaacacctt ctgaatatcc tatatcatct gccctaacta ctaatttctt
    12001 cattttatgc atctgcctgc tttctcaact catcaagttg catttccatc tcatccttta
    12061 ctttaacaac atccgttcct atgatgattt gaactgatgt attactcaat ttaattaccc
    12121 ctttggctcc tgttttttta atattatcat cattcaaaat agtagcatcc ttcacttcta
    12181 aacgtaatct cgtcatacaa ttattaagag tcactatatt ttcataacca ccacaatttt
    12241 taattatctg tttagccata tatgaaaaat taccatgaga aagttttaaa ttcttttctt
    12301 tttcactaac tttatcacct atctctatct caacttcacg tcctggtgtt tgcacattat
    12361 cttttattat tatatattta aatgttacat aatataatac aaagaataca actcctacag
    12421 gtataactaa ccacgcttta tctccataac taaaacttaa gacatagtca attatacttg
    12481 ctccattaaa tgaacccaag cgagcaccca gtaaatatag tacaactcca cctagaccag
    12541 aataaaaaga atgaattata tataactttg gagatgcaaa taaaaacgaa aattctattg
    12601 gttcagttac tgttgccata aaagaagtca atgctccagc ggtcattgca ccttttactt
    12661 cttgcttctt tttatcttgt gctgttttat atatagcata agaaatagca ggtattgaaa
    12721 acatcataat tatgtaaaat cctgtccaga agaatcctgc atttgggtct cctgccataa
    12781 atctaggaat ttctcctctt ataacttctc ctgttgatgt tgtatattca cccatttcaa
    12841 ataatatgta agcatttaaa acttgatgta acccagttgg tattagtgca cggttacaga
    12901 ataaaaacaa gaatattcct attgcaccag ctcctcccaa ccaaaaacca aattgattca
    12961 ttgcagcttg tggatatatc cataataaac taaatatata tgataaaatt aaagcaacca
    13021 ctggtgatag tgtaattaca catttttctc ctgcaaagaa gcttaaaaat gctggaactt
    13081 taactttata tgttttattg taagtccatg ctatacaagc tccaacaatt atcccaccaa
    13141 atacacctgc atttaatgta tcattcaata actctaaatt tgacttaaat atcatatatg
    13201 caactacggc tgcaaccatt acacttgtct tgtctttaga ctttgagtag gcactagctg
    13261 ctcctaataa aaataagtag tccacatttg aaaatactat attagatata ttagatatag
    13321 ctggcacatt gagcatattt ggaccaaata ataatcctaa tatacctgct gctggcatta
    13381 cagctattgg cactagcata gtctttccta aaagtgacag ttgttgcatc attttgttta
    13441 agaacctctt catatccaat cccctttcta tttcggttta attttttgtg aaaacgattt
    13501 gttcacattt tatattttaa atattatatt tgtttaaaca aattgtcaat attttttatt
    13561 cattttattt ttatcattta atttaacata taaatccttt taagtgtatt gctatatcta
    13621 gtctattttt ttaattcatt ttttttattt taaaaatatg tttaaccatg tatttgaaac
    13681 aacccacaaa ataattctat tttgtccttt ttatggttga tatgattcta ttttcactat
    13741 acatgtctaa acacatttga tataatttgt tcatatcagt tcaataatat tactaaagac
    13801 ctttaaggag ggataggggt atatggctaa aaataaattt tcagagatat atgaagtatt
    13861 gaaagaggaa atacttgatg gtaaatatac aagtaatatg atgctgccta ctgaacttca
    13921 acttattgaa aggttttcat gtagcagaaa cactgtacgt agagcaattt ctcaattaaa
    13981 cacagaagga tatgttcaaa gtataaaagg caagggtgtt gtagtgttag aaaatagttg
    14041 ttctaatgat ttttttctaa atatgcacaa tttcaaaggt gtagaatcga ttgtagaaga
    14101 taaaaaggtt aatactgcta ctagtgttct ccacttcagc aaaatcttaa ttgataataa
    14161 gttatctaaa aagacaggtt ttaaagttgg tagtgaagta tattaccttc acagactaag
    14221 atatattgat aacataccta aaattctcga cattaactat tttctatgta gtatagttaa
    14281 agaccttgat gtttctatag cacaaggctc tatttataaa tatatagaag agtgtctagg
    14341 tacaaaaatt gtttcatcca gaaagatatt taaaattgaa aaagctacag aacttgagtt
    14401 aaaaacgcta cctctaaatg attataattg tgttggtgtt attaaaaata gtgtatatac
    14461 tgatgatgga aagctttttg aatatactga atcaaagcat acacctgaaa catttgtatt
    14521 catggatgtt actcaaagat attaaaaaaa ttatgtttaa ttaaacataa tttttttacg
    14581 tttagacact ttatcccata ctaacctcac tattctatat acaattatat gtttatacaa
    14641 ataattattt attgtatatt ttagctttaa tattagttta acgcttgaca atttgtttaa
    14701 acatattata attaacttat aaattacagg aggtatcaag tatgaaaaat tggtggaaaa
    14761 aagcaactgt ctatcaaata tatccaaaaa gtttcaaaga tagcaacaat gatggtatag
    14821 gtgatataaa tggtattata gaaaaattag attatctgta ttatttaggt gttgatttat
    14881 tatggttgac tcctatgtac gtttctcctc aaagagataa tggatatgat atagaagatt
    14941 attataatat agacccaaaa tatgggacaa tgagtgactt tgaaaaactc ttaaaagaag
    15001 ctcataaaag agacataaaa ataatgatgg atatggtcct aaaccataca tctactgaac
    15061 ataaatggtt taaagaatct aaaaaaagca aagacaatcc atatcgtgat tattatttct
    15121 ggaaagatgc taaacctgat ggttctgttc ctaataactg gatatccaga tttagtggaa
    15181 ctgcttggaa atatgatgaa accactaacc aatattattt acatttattt gaagaaactc
    15241 aagctgattt aaattgggaa aatgaaaaga ttcgtgaaga atgttacaaa atactagaat
    15301 tttgggctga taaaggtatt gatggttttc gtttagatgt agtcaaccta ctctccaaaa
    15361 cacctggatt acctgatgac cctataacag gaccaaaagg agatggaaga actcactacg
    15421 ctgatggtcc tcgtatacac gaatatttac ataatatgaa tcaaaaagtt ttcaaaccta
    15481 aaaatatagt tactgtaggt gaaatgtcat ctactacacc tgaagagtgt atcaactata
    15541 ctagagaaaa tcgagaagaa ctatctatgg tttttagctt ccatcatatg aaaacagatt
    15601 ataaaggagg cgcaaaatgg actaatgaaa tgttctctct ggataaatta aaaaaagcac
    15661 aatctgactt tcaatataaa atgtatgaag gaaagggatg gaatgctcta ttttactcta
    15721 atcacgacca acctagagct ttatctcgat ttggagatga tagatttttt cgtcaagaaa
    15781 gtgctaagat gttagccata acactatttg gtctacaagg tactacatac atatatcaag
    15841 gtgaagaaat aggtatgaca aatgcctact ttactaaaat agatgaatat gatgatgctg
    15901 aaagtaagaa tgcatactat catatgatta aatctggtat tgatgaaaag gaggcattgc
    15961 ttatacttca acaaaaatcc agagataatg cccgtactcc tatgcaatgg gataatacta
    16021 tttataaggg gttttctaat cataaaccgt ggttaaaagt taataatgac aatattagtg
    16081 tagaaaatga gcttaatgat tctcgttcag tattttatac ttatcaaaga ttaatcaaat
    16141 atcgtaaaca atatgatatt tttacagaag gtacttatag attgttagat gaaaatcata
    16201 aaaatctatt tgtttatgaa agagagtata aaaaccaaaa tcttcttgtt ataagtaatt
    16261 ttactcatga taatgtagta tataaattac ctgaaactta tttgaataaa ttaaactaca
    16321 caatacttga aaccaattac actagagata taattgaaaa taaaatggaa ataaaacctt
    16381 acgaaagtat aatgatatat tataaataaa gttatttatg taaaaatcgg tataacaccc
    16441 tggtgttata ccaattttac taaattcaaa gtaatattta tagaatatct atagattaca
    16501 ataattattt gacttctaca tcattatatt tctttgaata tactttagtc atatagaaat
    16561 atataggaac tacaattatc atcccaccta atccccaaac tatcttcata ggttcatcaa
    16621 tacctgcttt tatcagtaat aatatactta taataactgc aaatattggt ataattgctc
    16681 ctccaggtat tctaaaattt acatctttac cagcatactt ctttctcaat attagtacag
    16741 ctaaacatgt aggaatatat tgaataaatc tagatacaac acttatttga gataatgttg
    16801 tgaatgaacc cgaccatgct attagtaaag ttcctatgac agatactata attgctatat
    16861 aaggagatcc atttttatta gtttttctta tagacttagg cattaatcct tgttcaacca
    16921 aagcagcagc agaccttggt gtaaatatgg atgatgcaat atttatacct ccaacagata
    16981 ctatagttcc tattgtaatt atatacccac ctataggacc taacatctta atggcagcat
    17041 ctgcaacagg agtagctgac cctgccaatc cattacccaa cacccctata cttattccca
    17101 gtataagcat atatattact gatactacaa acattaccat taccaatgct ttaggtacat
    17161 ttttcttagg gttctccata tcttttgctg caacagctaa agattcaaat cctgtaaaag
    17221 cataaaataa agttatagat actgctacaa aagctgatga cgcactttga cctttttgta
    17281 ttattacaaa tggggtaaaa ttagaaccct ttataaagaa taatcctaca gctataaata
    17341 aaactattgg taataatttt cctatagtca tgatattatt taagtgctta gattgtttca
    17401 tccccataaa atttataatc ccaagtccta caactaatat accaattatt atatttttaa
    17461 taagtggttc tgttgcttgt ggccatacag atgatagtgt tacagcaaag aaatttgcca
    17521 tagtagccca agcaatcata ctaactatcc acttcataaa tccaacttca aatccaacaa
    17581 aatctccaaa tgcctctttt gcataaagat agggccctcc atcagtatca aaccaactac
    17641 ctgcttcagc aaaacataaa gcaatagaaa cagctaaaag catacaaaaa actaatacta
    17701 atatacttgc tggacctata tctgtataag catcaccagg taacccgaat atacctgacc
    17761 ctattattgc attgattcct aatagtacaa tactccaaaa acctaactta atgccttttt
    17821 ccattgttat tccccccaaa gccaacttaa tatatttata ttttttattt agatgcttct
    17881 ttaactattg ccataaatat ttttttcata ttatcataat ccctagtcat catttctgga
    17941 tgccattgta tccctattac aaactcactt ccttcctttt ctatagcttc aattaaaccg
    18001 tctttagatg tagctgaaac aatatatccc ggtgctactt tgttgacaac taagtgatga
    18061 aaactattaa ctaaaacttc tttttctcct aatatttcat ataattttgt cccctccttt
    18121 atctgtactg tatgtgtagg tacattagat agatgttgct gattgtgttt aatataagca
    18181 ccttcaatta aagataaatc ttgatacaaa ctacctcctt cagttacatt tataatttgt
    18241 tctcctctac atatacctaa aactggtttt ttcatatcta aagcatgttt taataatttc
    18301 atatcaaaac tatctctctt tggtgatatt gcccccaact tattatgagg ttcttctttc
    18361 catatgagtg gatttacatc ttgtccccca gatagtataa gcgcatctat attagatacc
    18421 tgctccttta taatctcatc atcatataca atcggtacaa tatatggtat agctttacac
    18481 ataactactg attgtatata atcattattt acataagccc tttcatagcc aggaaacatt
    18541 cctccttcat caattagtaa attacctgat attcctatta ttggatattt ctttttagcc
    18601 aatttcttca ccccttaact tatttgtgaa caataaaaaa tgctacttct cttaaagagc
    18661 aagtagcata ccaagatttt atttatttga acattataat ttattttaga aatacttttt
    18721 ttgaatacga gtaatataaa taactttatt tacttatatt ttattttaat cctgtttagt
    18781 ttgacttttt taaattgttt taagtacaac taataggtaa aataggcatt tagattttcc
    18841 tttttatcaa cctattaaat actgtttaat atattttttc cccttctatt taattcactt
    18901 ccctttctac cagattcaca tattatcaaa tccagctctt ttaacatatt aagtattccc
    18961 cttacctcac cttctgttat attgtagcct aattcattta ttgaattaac aataccattt
    19021 ctacctatat ttttattaag attattggca ttttttaaac actccattac taactttact
    19081 ttctctatag atgttctatc ctttaatata cttatttctt tactaaaatc ttcagttata
    19141 tttaataagt tatgaggtaa atcatttatt aaaataacat cctcagaact cataatgtta
    19201 atatacatag cagtattttt taattctctt atattacctg gccattcata tgcattaata
    19261 atgtcttgaa catcttgact aattgctctc ttccttttca taaaatgctt taacataact
    19321 tctatatctt cttttcgctc tcttaaaggt ggtatattta taggcaatac atttatccta
    19381 tagtataaat ctgctctaaa tctatctcct tttatcattt ctaataaatt tttatttgta
    19441 gccgcaatta ctctaacatc tatatctatt acattttctc ctccaaccct cattacttga
    19501 ttctcttgta atactctaag taatttgctt tgtaagtata taggcatgtc tcctatttca
    19561 tccaaaaata tagttccatt gttagcctgt tcaaataatc cttttttacc tccttttaaa
    19621 gctcctgtaa aagcaccttc ttcatatcca aaaagttcac tttcaagtaa attttctggc
    19681 atagctgcac agtttattgc tataaatggc tgactatgtc ttggtgatgc attatgaata
    19741 gactgagcca tgagttcttt tcccgttcca ctttctcccg ttataagaat tgtataatca
    19801 gattcagcta ttttcatacc taatttctta gttctttcca tcttcgttga aactgttttt
    19861 atatcatcaa aagtatatct agcaacttgt cccttttctc taagcttctt tgataggttt
    19921 tgctccaatt ttcttatgta agttatttct tgaatacaaa aataatatcc agtaaccctt
    19981 ccaaaactct ctatattata cttatttacg tttaagtact tattattaaa ttttataact
    20041 tcatcatgtg ccttactcaa actaaaaatc tttttaatat tctcattctc taattcattt
    20101 atatttttcc ctataatatt actttgaata tctagtatat cccttaaact attattgcaa
    20161 ataattatgt ttcccttatc tgataccatt gccataccta agtttgataa atttattatg
    20221 gaatttagtt catctgcttt tatagtaagt tctttgtatt tagtatttat tcctgaatat
    20281 aaactaatta tttcatctgt atacttaatt agcctctcag taatttcttt atcatccagt
    20341 ttgagttttg ctattatttg aataaacgtt gaactatcaa tatacctatg gcctaaatca
    20401 ataacttctt ctatatattt tggaactaaa cttgactctc ctggtgttat agctatctta
    20461 atatttctat attcttcatt gggattgtat ggtataaaat tcaaatggtt tattcctata
    20521 ttgtataaat tagatattgt ttccaataca gtatgcttat tatcgtttac tactaataca
    20581 tctattcctt ctggcaatga aaataactta tatatatcct tttgctttat gctcctattt
    20641 atagttatta tctttgagac atcttctaca tattttttta tttttaatac tctatcatct
    20701 atcattacca atattatgtc atcttttatt aattccttct ctgttatttt atttaaataa
    20761 taatttttta tcattacttt atctttaaat atcatttcta gatttttatt taagaaacat
    20821 tctaatttag acaacccatc agttactact gctattgttt tcatttgatt actcctttta
    20881 aaatatactc tactctttta taatatacat ctgattttca ctttaatagc acaaaaaatt
    20941 gtacatttag tatactaaca tattccttta ttagttttat gtccctaaat atcatagttg
    21001 caatatctat catatttctt aaacttctat aaagcaaaaa ttgtgccaaa ttaataattt
    21061 tcttaatttt attctaaagt tcctcaatag tatttacaga cttagcactt atagcttata
    21121 agtctttata ttattaatct atttttatag gttaattgta caaatcatat atcactttta
    21181 cccattaaat gcattataaa aaagatggaa taacgattcg tcactccacc ttttttataa
    21241 taaaaaattg tatatataaa cactatttac tactcttcta agtacaaaca gtatctacat
    21301 actataacct ttcacctaca aacagtatct acacactata acctttcacc attagattct
    21361 attacttgtt tataccagtc aaaactcttt ttcttaatcc tagacaagtc tccacttcca
    21421 tcatcatact tatccacgta tataaaccca tatctcttgg ccatctcacc agtagatgca
    21481 cttactaaat ctatacatcc ccatggagta tatccaaata aatcaacacc atcttctaca
    21541 gcttccttca tttgctctat atgtgctttt aaatactcta tacgatagtc atcatttatc
    21601 actccattag attctttttt atcataagct cctaaaccat tttcaactac catcataggt
    21661 attctatatc tatcataaat ttcatttagt gcatatctaa gcccttcagg gtctatttgc
    21721 catccccatt cactagactt aagatatgga tttttcactc cacctaatat gttgccactt
    21781 gtattatctt ctttagaatc actacttaca catgtagaca tataataact aaatgtataa
    21841 aaatctacac aaccttcttt taaaatttct aaatctcctt tttccatttt tatttcaata
    21901 ttattttctc caaaatatct attcatataa gttggatatt cgccacgcac ttgtacatct
    21961 ccacagaaat aattcattac tctactatct ctttgagttt taactgcatc atctggatta
    22021 caagtataag gatacattgt tgcaaatatt tgcatacatc caacttgata atttgaatct
    22081 atttcatgag ctaacttaac agccttagca cttgctacaa attgatgatg taacccttga
    22141 aaacgaagtt gaggtatatc tacttggttt ataaaatctg tagtcccttc atttaatatt
    22201 ccttgtgata agtagcctcc cataggcatt gtagctgaat ttatttcatt aaatgtcagc
    22261 caatatttta cttttccttt atatctttta aatattgttt cacagtaatt catatacaag
    22321 tctattacat ctcttgaaac ccatccattg tatttctctg ttattgcaaa tggtatttca
    22381 tagtgagata ttgttattaa tggttctata ttatatttta aacattcatc tatcacattt
    22441 tcataaaatt taagtcctgc ttcatttggt tctttctcca ttccagttgg aaatattcta
    22501 gtccatgcta ttgaaaatct aaatacctta aatcccattt ctgcaaataa agctatatct
    22561 tctttgtatc tgtgataaaa atctattgct tcatgacttg gataaaatgt tcctttttct
    22621 ataactctcg ttattctttt acttactttt tgagaacctc cagtacacat atctgaacaa
    22681 gaaggacctt tcccatctaa attccatgct ccttcacatt gatttgctgc tgctaacata
    22741 caccctattt taaattcttg atttatgtta tgtgctagtt tagtaactaa cgcactcgca
    22801 actaattgat gatgtgcagc ttggtatttt atttgttctt cattttctcc ctcttcaata
    22861 tagagacctg cacctataaa tggtaaatga agtatcatgt ttatttcatt aaatgttagc
    22921 caatacttta ccttaccttt aaaccttgta aaaataactt cacagtattt tacatataag
    22981 tctactagct tacgatttct ccatgctcca tattttttta ccaaatacat gggaacatca
    23041 aaatgacaga ttgttactac tggttctatt ccatacttta aacactcatc aaataaatct
    23101 tcatagaatt ttagtccctc ttcgtttgga acatcctcaa ctcctgttgg aaatatccta
    23161 gtccatgcta ttgacatacg atagcattta aatcccatct cagcaaataa ttttatatct
    23221 tctttataat tgtgatacat atctattgct tcatgagctg ggtaatagtg ttcatcatca
    23281 cattcaaaca ttttcatttt cccagccaaa actggaaatc tatcttttcc ttgcggtgta
    23341 acgtcaacat tagctagacc tctaccatct tctttatatc caccttcaca ttgatttgct
    23401 gctgttgctc caccccataa aaaatctttt ctaagtgcca taattctacc tccaataatt
    23461 tatttatagt aactttattt taaaataaaa atacatatat ataattctta attaatagaa
    23521 ctctctttta cgctaaaaca gttatcaatt cctgactatt atttacatgc ttttgtgtat
    23581 tttctacaat atccacataa tcagaataat tagtaacaac tactggtgtt tctaagcaat
    23641 atccttcttt tgaaatactt tctatgtcaa aagttattaa tgtctgacct tttttaatct
    23701 tatccccttg attaacaaaa gatttgaaat atttaccttc caactgaata gtatttaacc
    23761 ctatatgtat caatagttca caaccattat cagatataat tcctattgca tgttttgttg
    23821 gaaataaagt cattacagtc ccatcaaatg gtgccactac ttttccttct ttaggtgtta
    23881 ttgctacacc ttttcccaat atgcctgttg aaaacgctgc atcttgtatc tgctccaaag
    23941 cctttacttc accttttatt gggctatata ctacttctct tttgtttttt tctataacgc
    24001 cttgcccatt atcatcttta gaaatttcta ctatagcatc atctttccaa aaaaataatg
    24061 ttaaaacaaa tcctactaca atagctacta ttattgctat tatagttgca taaacacctt
    24121 tcatatctcc agttgttgga tttatataat taggtatacc aaatattcca agtccaccca
    24181 ttacataact ttttacattc attatcccca ttacagctcc tgccacagca ccacctacac
    24241 aactatatat gaatggtgtt tttcttggta aagttattcc atatattgca ggctctgtta
    24301 caccacatat ccctgaaata actgcaggta tacacagctc ttttatcttc ttatctttta
    24361 atttaaagta cattgctgct actactgctg tttgtgcaaa agatggtaca aattctccca
    24421 ctaatattgt gtcaaacccc atagtcataa ggtttgacat agctattggt actaatcccc
    24481 aatgtagacc aaatattaca agcacttgcc acaataaacc agttaatgca ccacataaaa
    24541 ttggtgaaaa gttaaataaa gctaaaaatc catttccaac tatattagaa gcataggtag
    24601 ctataggccc tataaccata aatcccaatg gtaaagaaac taataaaact gccattggta
    24661 caaagaaatt ctttactaca tctggtatta ttttagcaaa cactttctcc aacttagctg
    24721 ctaataatac tattaaaatt actggtacta ctgttgaagt gtagttcatt gctattactg
    24781 gtaatcctaa gaaagttata tatactggtg attcaaatat tgttccagta aatagattgt
    24841 ataaaggttg acctgtagat gctagagtat ctaattgaat acctgaatag cataaaactg
    24901 cacctataag cattccaata aatggtttta atttgaattt cttagcagct gtatatccaa
    24961 gaattattgg catatactgg aacattgcat ctccaattgc atttaacata atatatgcac
    25021 catcagtttc tccatacaaa cccaatgctg taaatactgc attaaatcct tttatcatac
    25081 cagcagcaca cagaactcct aatattggtt gaaaaactcc tgatattatg tctataaact
    25141 tattaagtaa tccttttggc gcatctgaat ctgaattatc tattgtatct cctcctaatc
    25201 cagccaaatc gcacacaact tcataaacat ctggaacatg attaccaata actacttggt
    25261 attgacctgc acttttcatt acagtaacta taccatccat tttttttagc acatcatcat
    25321 ttgctattga ctcatctttt aatttaaaac gtagcctcgt tatacagtga gataaactgc
    25381 taatattatc ctttcctcct acattcgcaa tgatttcttt tgatagttgt tcatactttc
    25441 tcattttttc tcctccattt tctttttgaa atatgaaggt ttggccaact taatgtcacc
    25501 acccaatata tataaaccta attattagga tttatatctg acttaatact tttacttacc
    25561 actctttcaa cgtgaatagt taagtataat ttttcctcat ctgttaaatc gtagttgtat
    25621 tttttatgta taaattcatc caacttttct atgaatttat aagcattttt atatttaact
    25681 tttattacat ttaataaatc atcttcatta ttttcatgta aattattact aacaattcgt
    25741 tctgcaaaat actttaagtg agtaataaat ctatgataat aaactgaatc ttcatcaaat
    25801 actattctgt aatggtattt aaccatattt gaaatttctt gtattatttg tgtaatctca
    25861 tatatagttt ttatactttg atccatttgt gcattaacta tatgcagtgc tataaatcca
    25921 gcttcatctt ctgatagtct aatttcaaat tttttttcta tataatccaa tgcctctaac
    25981 cctattttaa attcactctt ataaaatctt tttatatccc aaagtaatac attttttaca
    26041 cttactcctt cacgagctct ttctattgca gtatacatat ggtctgttaa agaaatataa
    26101 atactatcat ttaatctttt ccctagtttt tctttagcat ataaaattat ttcatctgat
    26161 aattccatat atcttatagg aatatctgct attagttctt taaatttatc agatatattt
    26221 gggtctgaaa gtttgaatat cttatcaatt ttatcctttg atatatgctc tcctgtacgt
    26281 tttttataag ctaaacctct ccccataact actatttcct tatgattttc atcagttgta
    26341 ataattacat tattattgag tattttttca ataatcattg tgatgccccc tttattagca
    26401 ttaagttaaa ataaatcttt tttaattatt tagaatattt aaaatgtaat ttattacata
    26461 aaaaaaaacc tatttattac acattaaata gactcccatg agtcctgagt aataaatagg
    26521 tttagcttgt atttaaacaa tcactatccg tttgtatttt attttaatta ttatttatta
    26581 tatgttagca aatttatttt ttagtgtcaa ctttttctca atattttaat tttaaataaa
    26641 ttatataaat tgaatcttca aagtactatt tttatcaata aacaaaataa aaaaggtatt
    26701 actttcttta agtaataccc atttattttt aatttaagtg gtttttctta atctcatcaa
    26761 gtagtccatc ttctaaataa atttctaatg cacatcctac cattgccttt actgctttgt
    26821 gaagtttatc ataagcttcc tcagaatttg cataatttag aaattctttt tcatgaacat
    26881 ataccattgg acttatatct aaggctattt ctgtatacat agtagggcaa acttgactta
    26941 cattacctat atctgtagaa cctactggac catctttacc ttctaatata tctgttatac
    27001 ctgcctcaat caaattattc tttgttatct tttgaagtac tttgttattt attatgttgt
    27061 caaaagaatt ttcaaagtat ctataagaaa gcttagctcc tgtcattaac tctgcacctt
    27121 ttgcacagtt tataactttt ttagttactt catctaaata acttctttca gcagctctta
    27181 cgaaaaactt acaggctgct ttttctggaa ctatatttgg agcctctcct ccatttgaaa
    27241 ctataccatg tattctaaca tctgatttta catgttgtct tagtgcattt atcccagcaa
    27301 aagtaagttg tactgcatct agtgcattga ctcccatatg agggtgacta gctgcatgtg
    27361 ctgatacccc ttcaaaacta aactctaatg aatctatcgc caaagcctta cagtttatat
    27421 tattaaaagc ttctatatgc atttgataac ttacatcaac atcatcaaaa gctcctgcct
    27481 taactaaatc acattttcct cctgttgttt cttcagctgg tgttcctatt agtacaattt
    27541 ttcctttaaa attttctttt aacttactta atactataca agctcctagt gtagatgctg
    27601 caatccagtt atgtccacaa gcatgagctg gttctttatt ttctccatat ccaggaagtg
    27661 catcatactc agctaaaaat gctatggtag gtccatcatt atctcctaat tcagctctaa
    27721 aagcagtctc catattacaa tatggataaa ctgtatcaaa tccatattcc ttcatttttt
    27781 ctactaagta ttttgatgat atgtattctt cttcccctaa ttcaggattg ttaaatataa
    27841 aattacaaac ttcttcaatt tcttttccaa gttcattctc tatctcataa gctcttttct
    27901 ttatactctc tatcataatt tacctcctac tagaatccta gatataatcc tattgtcaaa
    27961 aatgccatta caagaattgt taatactacc attaaaggaa ttacgaatct aacccacttt
    28021 gaccaaggaa tattggctac tgctagtgct cccattaaaa cacctgatgt aggtgttatt
    28081 aagttagtta cacctatacc aaattgataa gctgtaacta tcatttgtct tgatacatct
    28141 actaagtcgc caagtggtgc aagtacaggt attgtaagtg atgcaagccc tgatgctgat
    28201 ggtattaaaa atgctattaa tccttctatt aagaaagtta aatttataaa tatagttttt
    28261 ggtaatccat ttaataaatt tgctgcagaa tttaaaattg tatctattat aaatccattt
    28321 tctgctatta ttacaattcc acgtgcaaat gctatacaca atcctgctgt cattaaatct
    28381 cctgctccac ttataaatga attaactata tcatcttgtt taagacctga tgctatacct
    28441 gatattatac ctatagctgt aaatatcatt gcaatttcag ttatatacca tcccaacttt
    28501 aaaactccat atatcataat cgccatacct attgtgaaaa ttgctattac catggcttct
    28561 ctttttgtaa attcctttat atttgtatca ctattaacca ttgattcttg atttagtgat
    28621 atatcatgta ctaaagattt tttagggtct tttttaactt tattggcata catcataaca
    28681 aatcctatag atatagccat atatattata aacataacaa ctctaaactc tattccagaa
    28741 cctggtgcta tttgtgataa tgcttgtgct attcctacag agaaaggatt cgtagtagct
    28801 gctccaaatc cagctgttgc tcctataaat actgtggcca ctgctgtcaa tgaatcatat
    28861 cccatactgg tcataagtgg aataaatatc atataaaaag gtagtgtctc ttcactcatt
    28921 ccaaacgtcg tcccacctag tccaaataga atcattgaaa tcggtattat taaaaattga
    28981 aacttcttca ttttatttac agtatgagct ataacagctt ctatagctcc tgttttatta
    29041 actatcccaa aagctcctcc aactattaaa acaaatccaa ctacttcagc tgcatctata
    29101 aatccgttta taggtgctgt aaatacatca gtaattcctt gtggatttga tggtgctttt
    29161 tcataagttc cagctacaac aactgacctt ccgtctatat ctactctttc aaaatttcca
    29221 cttggcacta tccaagtaag aattgccatt aacactatta gcaaaaatat tattgtaagt
    29281 gtctgtggca tagtaaactt tttctttttt aaactgctca tttttaatct ccccactttt
    29341 taaattaaat attttattat tgcatccctt tatcctattt tgtatagatg taaaaaagct
    29401 caacatggat acatgttgag ctaaatgtgc taaatacaac atacctgcat cttcccttta
    29461 tcttgtttaa gatagctctc catcaaaatt cgggataatt ttgatgacag tactgtacct
    29521 attaaataca gtcccagcac tagcttcgga cactagcact tcggcactta ctccttttat
    29581 attgcctcat tggtttccaa cctcttcaat attaatctta gtaaatgcaa cctctacctc
    29641 aatctgtttg ttagatgagg taattcttat taatttttat taattcaaag taattgtagc
    29701 ataatatgtc gagattgtaa atattttcag tataataatt cttactttat gatttcattc
    29761 catttttcac aaataatttt ttctccatat aatttaaata ctgtagtata ttttaaatac
    29821 acacaaactt tccacaaatt ttaaattatt ataatacctt tataataaaa aataaaagct
    29881 atgtaaactt atcaatttac acagctttat atacattaat ctactccaag tacagcttta
    29941 agctttgatg ctattgtaat aactcgtgga ccaaatataa cttgaactga gtttttcttt
    30001 ttaacaactc cacttgcacc caattcattt acccagaagc catcttcata gaatccatca
    30061 attgaattta gctctaccct aagtctagtt gcacagtttg taacattttt tatattatta
    30121 cttcctccta aaccttctat tatagaatgt acaagctttg tatctttatc atctacttcc
    30181 tcttctactg cattgttgta tgctgtttta ccagcatcta cagattgtct ttctctatat
    30241 tctttcttac tataaagctt aacttcttct gaacttcttc ctggtgtttt tatatcaaac
    30301 ttcttgattg cccatgtaaa tattatataa gtaactataa aaaatattgg tatcaaaaat
    30361 aataatggta acgcatccac tttttgtggt tgtaataaat taggaaacat atttctaaat
    30421 gattctccca ttatagaaac attaaacatt tctgtaaata catacgtcaa tcctgccagt
    30481 ggtacatatg ctacaaaata taataatggt gcaacaaata ggaatgtata ctctattggt
    30541 tccgttaccc ctaccaacat ggctgttact attgctggta tcaatatact tattgctttt
    30601 ttcttattag cattatcagc caacttatac atagctaaag ctgctgctgg taatattgca
    30661 aaatgtatta ataaacgacc tgttgtgaag ttacgagtta tataagatag tgtatctggg
    30721 tctcccattt gagctaatct tatattatta acaccctcgt aaattgctcc acctacttcc
    30781 attgttccac ctactcttgt atattctata gggaatgcaa ttaagtgatg tataccaaat
    30841 ggtaataatg ctttatccat tgctccaaaa gtaaatgttc ctaataatcc actagaacct
    30901 atccaattac caagtttaga taatgccaac cctactggtg gccatataaa gtacattgct
    30961 gtacccaata ctattgatgc tataaacatc ataatcattg aaaatcttgg acctgagaaa
    31021 aaagataatg cactaggaag ttctatgtca acaaatttgt tatgtactgc actagctaca
    31081 aatccagcta ttatagctcc aaatatacta caatcatatg aaaagattcc taagaaagaa
    31141 ccatataaag aacttcgact atatgcttct gcctctccta atccagatgc cataaacgct
    31201 tctgctgtaa ctgtatctga atttatacca tttgctgcta acaatgtact tataactgtg
    31261 tgcattccta taaataaaac aattcctcct aatgcacccc atcctttttc ctttttagat
    31321 aatccaaagg ctatacctgt aacaaagaat attggtaagt atctcattac cataaatcct
    31381 aaatcgttta ttactttaaa gaatacattt ataaatgacc cttcttttat tagtccgcta
    31441 agccctaatg cagaaacgtt ttccatattt gcaaaagctg accctaatcc tataaatact
    31501 cctgctacaa caagtaatat aacaggtatc atcattgctc cagaaagtac ttgtaattta
    31561 tccttcatag tattatttcc tccattttta ttgaaatgcc aggctatata atgcatatat
    31621 atatacttct attgctttgt ataatgtatc tatacttata ttttcatttg cctgatgtga
    31681 atttggatta tccccctcta ttgtgggtcc aaatgcaact ccatttttta acactcttgc
    31741 ataagatgca gctccttttg taaaaagttc ggcttctttt ccaaaacctt gtttatatgc
    31801 acttgataat ttatttatta attcactatc tggttttaca tacagtaatt ttaaatcctt
    31861 atatatatca actattaaat tttctttttt tccaatttca ttaattctat tagttataaa
    31921 ctcccaactt atacccttag gatatctaaa gtctattttc atattcaatt catttttctc
    31981 taagaagata ctcattatac atattgtaga aactcccata tctacatctt ctttatatat
    32041 ccctaatttt tttccatgat tatcatctgt aaagtatgaa tttaaaatat cccttattat
    32101 aacgccttta tcatttagtt ttttaatttt ctctaaatct tttgctaaac taaatgcaca
    32161 gttatatgac ctgtgtggat ttctcgataa agccctttct cctgtatatc ttactaatac
    32221 tttaccttct tctagttctt ctatctctgt ataataagct aatgattcta ctaaatcttt
    32281 cttatcatta gttttaaaaa cagcctcaat tttgttacat acaaatccat cttctttgtt
    32341 tgactttata tcaactaaat tgtgatttct aaatttatct ttatctattt tctttcttag
    32401 attgaagtat aaaatacctt tttctccatt tactattggg aagtttccat ctggagaaaa
    32461 tgcaaatttt ggctgttcat tgacactaaa gtaatgctcc atacattccc aagtcgtctc
    32521 ttcagctcca cccactatca atctaatctt tctcttcggc ttttcattaa gttctcttaa
    32581 gaaaagtaat gcatataaaa tccctatcaa aggtcctttg ttgtcattaa ctcctctacc
    32641 ataaagatag ttatcttttt gacatacttt aaaaggttta ctcttccaaa gttctttatt
    32701 atatattggc accacatcta tatgattaag cacccctact acttcttctc cttctccata
    32761 ttctatatga attgcatatc catcaaagtc ttttacaaca aaatctttgt cttttgctat
    32821 ttgaataagc ttatcgaagg catttcttat ttctatccca aatggttgat ttatatctgt
    32881 tgtactttca tctctaattg aaggtatttc aactagactt ctaatatcat ttatgatatt
    32941 ttcttgattg gattttaaat aatcaataat tagttctttg tttactatca tcttaattat
    33001 ttatcacccc tttatattaa ttttacaaag catttcagtt catatctaca ttataaattt
    33061 tgaatattat tttttcaata tttcaaatac ttctctaagt taccataatg atacggtaaa
    33121 caatcaacag attatgacaa aatcatacta tacctattac taatatttta acttatctat
    33181 tacgtctttt ttaatgtaag tttattccaa aaatacataa aaaaagatgt agccttagct
    33241 acatcttctt atatctattc aacttccatt attatgctat taatatctac atttccaggt
    33301 tctatcctat cttttactgt tctcttaaca gaatcagcat catccattat aactacagga
    33361 gttattatat ttaaatcatt tgacgttata aaatctaaat cggcttccat tattaaatct
    33421 ttagctttta ccttgtctcc tgcctttacc ttaacatcaa acggctttcc ttctagtgac
    33481 attgtctcta aacctacatg tattaatata tttgtattat cattactctt aatcattatt
    33541 gcatgctttg tatcaaatac attgactatt tcaccatcaa caggagaata taacttacct
    33601 tcacttggta ttattgctac tccatctccc atcatctttt gggcaaaaac tgcatctgga
    33661 acatcttcta agttcactaa atttccctta aaagggctat atattttctc aatcttattt
    33721 tcttttttct taaatatatt aaacataaca cactctccac attataaaat tttatgataa
    33781 tatttttctt aaagcttttg ctacaccatc atttacatta gtatcagtta caaaatctgc
    33841 catagatttt acttcatctg tagcatttcc catagcaata ccaagacctg caaatttaat
    33901 catggatata tcattttcat tatcacctat acaaataact tcttcgctat ctatacctaa
    33961 tatatcacaa aattttttaa ctgcaatacc ttttgaagta cctttactca taacttcaaa
    34021 gtttatcttt ccagaactta ctacttctaa atcttcttgt tttttaaatt cttcctttgc
    34081 ttctctcaaa gaatctacat tttctgagaa aagtatacat tttaaaattt cattatcagc
    34141 tttttctaat aaagtttcac aacttgcatt ttctattatt tcaattctgt cttcttcttt
    34201 atttttacta tttattaatc tatatggatg ttcttcacct atttcattgt tggatataac
    34261 tattttacag cctttaaaat gtttgtttaa attatacttt tcacctattg tatagatttt
    34321 ctttagagct tctttgctca aaaccttctt atactcatat cccagtaatt taaaatatgt
    34381 cccattagtt gatataatat atatatcatc ccctaaaaca cttgtaaaat atttcatagc
    34441 attataaggt cttcccgtaa ctaaggctac cttgataccc ttttccatgg cttcttttag
    34501 tgctttaata ttttcttctg atacttcaaa tcctttaccc atcaaagtac catccatatc
    34561 agtacaaatt aatttatatc gcatttgtat cacatcctag taagattttt ctaatatttc
    34621 aacaaggtct tctttctttc caaaaggtaa aaatcctaaa actggacagc ttgttatttg
    34681 ttctaattgt tcctttgtta cattaaactc agacattttt gtcttaagtc ccactctctt
    34741 taaaaaactt tctatttcat ttcctaaagt tttagctgct tcatcatctt ccactttttc
    34801 taattcttgg tttagcattc tcgctacttt ggcaaatttc tctatattat ccttatagaa
    34861 cttttctgca aattttggga acactatagc caaagcctca ccatgaggaa tatgtgtaac
    34921 tcctcctatt atttcactta gtggatgtgg tgctgctgct ccagcatttg ccagtgaact
    34981 tcctcctaat gtatctgcta ctgctaactt tgttctgtat tctatattat ttttatcttc
    35041 cattacttta ggtagatagt taatgactaa ttccatagat ttttcagaca ttatctctgt
    35101 aaaatcatta gcatttggat ttatataact ttcaaatgca tgggtaaaag catcaaatgc
    35161 tgttgatgca gtaattctct ctggcagagt taacataagc tctgggtcaa ctatacattc
    35221 ttttgagaaa caattttgat gaaatattgt atttttctcg cttcctctcg atattactgc
    35281 tgcttgtgta acttggcttc ctgttccaga agtagttgat acagctatta acggtaattc
    35341 tttttcactt accttatcgt atattttaaa tggatttgta tatgtactaa acatataatc
    35401 ccaatctatc ttttctaaac caaaagtcaa tgctaatact tttgcagtat ctatacttga
    35461 acctcctcca acagctaaaa caacatctac agattctttt ttagctaatt caaacccttc
    35521 atttattaat tctacagttg ggtttggctc tactctatca aagtgtacaa cttctatacc
    35581 tttttctatt aaattcaatg taactctttt atataacttg tctaatggct ctacatttgg
    35641 agtagtaact aacaaacatt tctttccata ttttcctatt attctaggta attcacttaa
    35701 agttccttgt ccaaaattta ttcttgatgg ttgataaaag ttaatcatat ttttcctccc
    35761 ttaaattatt gccttcatat tcaaattata catatcatag gatttttaag gcaatacctt
    35821 cactgttaac tcttatttac taaagcttat ggcaattatt agacaatcaa tgttttatta
    35881 ttaccatatc attatgtaaa ttttattaag tacccatgtt ataattttat aatcaatctt
    35941 attgtataat tgacttaata ataaaaatag agtatataca cttttttatg acaaatagct
    36001 taaaggagaa ttaaattatg aaatatttga tagacagtgc taatcttgat gaaattcaaa
    36061 agtgtttaaa gtatggattt aagggaataa cagctaatcc atctatgtat cttaaagaga
    36121 aagtaaactt ccatgaattt attgagacat gtgctaattt aaatccagaa atattaactg
    36181 ctgaagtcat tggaaataat ttatacgaat taattaattc atgtgataag atattaaata
    36241 taaataaaaa cactataata aaaataaatt tctctgaagt tggcttagaa cttataaaca
    36301 tattaaatag taaaggtgta aaaactgcat gtaccttgat tttcaatgta aatcaagcat
    36361 cattagctat aaatgcaggt gctgattacc tattcccttt tataggtaga aatgatgaaa
    36421 atgggaatga tggtataaaa aaccttacag aaatatgtaa ccttatatca ataaatggat
    36481 ataatactaa agttgttgca gcttctatta aaaatgtatt ccacttaact caagcatctc
    36541 tttgtggatg tgattatgca gctgtaacat atgatttatt taaaaaagct tcttttaacc
    36601 agcttacaat agatggtgcc aatacttttg aaagagattg gtccaaggtt aataaatagt
    36661 aactaagatt taggagggtt tgcattgaac aaaagattat tagatattgc tgaattatta
    36721 ctcaacagct cagactatat tactgtagat actattgccg aagaattaaa agttagtaat
    36781 aaaactattc gtaatgacct tgttatttta gatgaatggc ttttagagtt taatttatcc
    36841 ctagataaaa aaactggttc aggggttatt attctaggta atgaagatat aaaacttaaa
    36901 gttattagag atatcaatga taaatcaaat tgtatatatg catactctcc tgaagataga
    36961 aaaaggtata ttctaagtaa attatttatt aaaaattcta agtttaggat tcgtgatatt
    37021 tgcaatgaac tacatgttag tagagctact gtgcataaag acttggtagt tattcaaaat
    37081 tttttgcaaa actttaaagt gactctagtt agaaaaacca ataatggagt atatttagag
    37141 ggaaaagaga aagatattcg aaaagcaatt tttgaacttg ctactgttaa taaaagttat
    37201 actgaactta aagaaatatt attctcgaaa tctaatgaat gcaaagatac cccatcaaca
    37261 aaaatattta aggagctatt taactatgat tttgagaaat tatctcaaat cactctacac
    37321 accttacaag tagaaaaaag taaccttagt gatgagtatt atataaactt cttaatacat
    37381 atagctatat gtattaaaag aatttcaatt aaaagatata ttaacttatc tgaaggtttt
    37441 tttagagaat tattagaaca tgaatacttt gaaaaatcaa aaattttatg tgctaaccta
    37501 agtgaagctt ttgacgtaga atttattgat gaagaggtct gttatatcct tcttcatata
    37561 gaaggattac ttaaatcaat aaagaaaact actactcttg aacctcttaa aaatgaagaa
    37621 attacagagt ataatctatc tgaagcaata gctgttcatt ggggaagtat actaaattta
    37681 aacttaaagg acgatttaat attaataaag tctcttgcta atcaccttaa atcagttcta
    37741 cacagaataa actatgggtt tactattaca aatcccatat taaatgatat aaagagaaca
    37801 tttccttata cttataaggc tgctaaagag tctaatactg ttatcaaaca gttgatgaat
    37861 tatgaagtca atgatgacga aataggatat cttgctttat atttaatatc agcaatagat
    37921 agaagcaaat ctcccctaaa tactatttta atatgtcact gtagtgatgg tgtatctaat
    37981 ttattagtcc aaaagttaag ttttgaattt aatcagatta atatagttaa atctatacct
    38041 ctatcttcta tttcatttac gaactttgat gatgtggact taatactaac cactgctcct
    38101 gtagactttg aacataatgc tgaactgata aatataaatg ctcttttaag taagaatgat
    38161 atttctagac ttagtacaat tataaaaaaa ctatattctg aaaaaaataa aatattatct
    38221 tataaataaa caaaaatagg cttgtctcaa catagagata aacctatttt tcattgtcca
    38281 aatgataaaa atatatttga tttcatagaa aaagtgcctc atgaactaaa aaattcattt
    38341 tgagacacct tcatttttta tatttaatct ttttatttaa gcttttatat tttttaaact
    38401 tttatattta aagtaaattt tattattttt attgagcaca agcattattt atattattaa
    38461 tttcttcagt attcttacca aactttgtag ttacaaatcc tattgttttt attatgagta
    38521 ataaggatat caatgaatat atagatatca taaaattctt acttattatt ccagacgcta
    38581 ctattataaa tccatctagc ataaataata taactggcaa cttcagaaac ttaattacct
    38641 tattaataag cattgcaact aaatcagttc caccagtact acagcccctt attattgcta
    38701 tccctatagc agttcctacc aataacccac ctatcactcc tgctactaat agattgtcag
    38761 ttatgtgagt ttgtggtatt acttttaaga ataatgggcc cataagagtt atatataatg
    38821 tttttattcc aaaacctttc cctaaaaata ataatcccat tactaaaagt ggcatagata
    38881 ttcctaatga tatattctca acaggcattg ataagaatgt gctaagtacg actgataatc
    38941 ctgttatacc tcctggagct aatgtatgtt ctgcaaagaa aaaattaact gctaatgcca
    39001 ttaaaaatgc tgatattgtt atcagtaaat aatcttttat catttctttt ttcatttaat
    39061 aacctcctat gattatattg tttcagtata aaaaatcaat taaattagtc tagcttcctg
    39121 tagtttttca taaaattaat gcttccttaa aacattaaga aagcatctct tttatcatag
    39181 tttattatct tattctaaac caaactttgt agctgcttca tctatagctt ttctacaatt
    39241 cttaacacct gctgttatac catctttatc cttgaatatt gctgaagata atattacaac
    39301 gtctgggtta cattctatta atggctccat attgtctgct cttagtccac catctattgc
    39361 taattcacac ttaggattct tttcgtctat catttttcta gctcttttta ctaaatcaac
    39421 agcactcttt ctccatcccc agttatcttt tccatcagtt tcatcaacac catgaacgac
    39481 tatttgcaat ctatctatat catatatagc ttcatcaaca aaacataatg gtgtgtaaca
    39541 tccaattgtt aatcctactt tcataccaaa ttctctacag taatttatta tgtatgcaag
    39601 aggagcacct aaaaaatgtt ctgctggtat tatcaacaag ttacatcctg ctgctgctat
    39661 tttttctata aataatctat cacaatctct tgtgtatata tggcattcta ttggtttatc
    39721 tgtcactggt cttattgctt ctattatttg atgaccaccc ataagttgca tattttttaa
    39781 gtcatgcata tctgctgcat ctgaatgtac gtaatctaca cctgcatcca ttgcttcttt
    39841 aactacggct gatatatgac cgtaatttac atgtgctaat cctgctgcta tttttatgtg
    39901 tttcatattt gtatacctcc atattttact ttaattagta ttaaatatta gtttttgata
    39961 tttttttaat attttttaat ccagtattac ttttatttag cattactttt attgctgtat
    40021 atatttcttt tatgaactta ttctgctttt gttaactgtg aacttatttt gctttgtaaa
    40081 ttaatattac actgatatat tttttttcac aacactttga ctactcctta aaagtacctt
    40141 tatattgtgg taaattatag acattaattg tatataaaaa gttagttttt taccacattc
    40201 ttatggcatt tattgtctat actaactcaa ttgatatagc tagttcattt aatatataat
    40261 cagactataa atataaattt actcgataaa aagtaccatt cataacaata ttaatgaaag
    40321 ggagatgtaa aatgagaaac tgtattattg cccaatctgg aggccctaca gctgttatca
    40381 atgcttctgc agttggtatt tatgaaaaaa atttagaaac taaatacttt gataaagtat
    40441 attttggtat caatgggatt gaaggtatat taaacaaaaa tataatagat tcagactcct
    40501 taaacaaaga taatgtatta aatttaaagc aaacaccttc gtctggtctt ggttcttgta
    40561 gatacaaact ggctgattat aaaataaatt cttatgatta tgatagagtt ataaatatac
    40621 tagatgaata tgaaatagat acattatttt atataggcgg aaatgattct atggacacag
    40681 tgatgaaatt ttctcaatac tgcaaaaaca ctacttgtaa aataaaattt ataggtattc
    40741 ctaaaacaat agataatgat ttattaggaa ctgacaactg tcctggattt ggttctgctg
    40801 caaagcttat ttctacacta ataatggaaa catacttaga tttttctata tacaaaaata
    40861 atggtatatt tatagtagaa actatgggta gagatactgg atggttagca gcttcttctt
    40921 cactagctag acttaatgat actccacttg ttgatttaat ataccttcca gaaattgatt
    40981 tctatgaaga taagtttata gaagatgtta gaaaaatatt taaagaaaaa aataaggctt
    41041 atatagttgc atcagaaggt ataagggata aatacggcaa ctttatatgc gaacaaaaaa
    41101 cttctgctca tgataaattt ggtcattccc aattaggtgg agtaagcaac tatctaaaga
    41161 atcttatagt atcatcatca attacaacaa gagtaaaaac cttagaactt ggtgtaatac
    41221 aaagatgtgc tatgcattta gcttcagata ctgatttgaa acaagcagct gaagttggtg
    41281 catatgggat agaactatct aaaaaaggat atacaggagt tatggctatc attaatagaa
    41341 catgcaataa tccttataag tatgaagtta gtcatactga tatttctaaa atagctaaca
    41401 agacaaaata ttttcctaaa gagtggataa atgaagaatg taattatata actgatgaag
    41461 ccctaaatta tctatatcca cttattcaag gtaaaccaga tatcaaatat aaaaatggac
    41521 taccttattt ttcaattatt tactaggaaa ttattataat ttgatataca ctcaattcta
    41581 tcgctcttaa atacctaagt ggctgttaaa taaaattata aattgttata tgacagtcac
    41641 ttaatcgttt aattatttaa ctgtttagtt ttttaatttt gtttaatact aatacaattt
    41701 gttgtaataa tatctcacac ccattttcat tttggataga ttataattag ttagacaata
    41761 aatattttgt catgatatta ttatatatag tgatacaaat agaaatactt ttatcatggc
    41821 tagaaaaaca aagattacac aaaggattta aaagtcaaac aacttcaatt aaaataaatg
    41881 gttaaaataa cttctaaatc aactacataa acaataaaag aacataaagt agttaaatct
    41941 acaattataa aattagtaag actataataa cttaaataaa attaaagtta acaaagacta
    42001 ttaaagttag caaatattcc tctacctaaa taattgcttt ttataaagag ttatttattt
    42061 taattgaatt taattgaaat acgattttaa ctagaacaat agattaatgt aatatttaat
    42121 ataaaataat tataataatt gaaaaggaag taatctatga tgaaaatagc agtcataaca
    42181 accgaatttt taaaagagtt tgtagataat tctataaaaa agttaaatat taatgcagaa
    42241 attgaaatat atatctatag agatttttct catgttggag atttatattt ggaaatagaa
    42301 gataggtttg atggatttgc agttagtggc cccatcccca aaaaagctat tacaaaaaag
    42361 gctggaacta ttaaaaaacc tctagttgat tttggtactg acttacaaag ttactatgat
    42421 atgtttctaa agcttatctt taaatataat acattagatt tttctagagc atactttgac
    42481 ttaacagact gggcagataa tccaaaagat atatcatatt atttatcaaa tggcacattt
    42541 ggagacttaa tggatagtat tgatgaaaat gcatctaaaa tgagtttatc tgacatagca
    42601 aaacttgaaa aaaagattgc aaaaaaacat attaatctat ggaatgaagg gaaaatcgat
    42661 ttttctacta ctcgttttag tagtattgtt cctatgttac aaaatgcagg agtaaacttt
    42721 catttcatat accctgatat agatttatta aaaaatactt ttaataactt aataaaagat
    42781 attcatttac atagaatgaa gggaaatcaa ccaagtgtaa tatatataac agttaaagat
    42841 acaaattatg atgactctac tctagaccaa gtttataaaa tattaaatag tattaaaaaa
    42901 gataaattaa ccgattttat aattgaaaaa aatagtaatt cttttaaaat tttcactaaa
    42961 tgtgaaacga ttaaatcatt aactaaaaat tttactgcat gttatataaa agaatctatt
    43021 gaaaatagta caaatattaa agtatgtgtt ggatatggaa ttggtaatga tataaagcaa
    43081 gctatgtcta gtgccataag tgctaataag gaatctaaaa taaatcattt aaattacagt
    43141 ttcttagtca acgaaaatta tgagacaata ggacctttac tgtgtgataa gttacttact
    43201 gtttcaaata aagtaactcc acatataaat acaattgcca aaagttctaa attgtctact
    43261 cttactattc aaaagattat atctgttact gaaattctac aaagagatga agttactcct
    43321 aaggaattat cttcatgttt aaatgtaaca gttagaactg ctaatagatt tctaagtagt
    43381 ctcttaaagt ctggaaacgc tactatatta tatgaaaaac aaaactcaac aaaaggtaga
    43441 cctgaaaggg tatacaaatt atttatatca gaaaataaat attaaaatcc aaaatgcata
    43501 aaaatatttt atcatctaat attaagtaaa attatatatg actttctaaa aggtatatct
    43561 ttgcatcttt ttaagatata ccttttatga tatttaataa tattttatgt tattatctac
    43621 taaaattatt ttctaaccat tttactgcac aatgagcaaa taaagctgaa ccataaacta
    43681 atccctcttc attaaaaatt gctttaggat tatgcatacc aaaattatat ccctgactaa
    43741 tacttcccat tccaatccat aaaaatactc ctggaaccct tagtaaaatc tctgaaaaat
    43801 cttctgaacc cattgcagat ggaaagtcta ttacattttc atttcctata acttctttaa
    43861 cataagatac aatctcatct cctacatcca aatcatttat aactgcagga gtccccattt
    43921 taaattccac tttagcattt cctccaaatg atttagctat tcctgtagat atttcaataa
    43981 ttctattttt taatttttct ctaacaaatt ggtcaaatgt tcttaaagtt cctgacatag
    44041 atgctttatt tggtatagca tttggtacgt ttcctgcatt aaacatacca atagttaaaa
    44101 ctacaggttc tttctgaggt ttttctcttg aaagtatagt gtttaatgct atttgtatat
    44161 gcgacattat gtttattggg tccaccccaa attcagggct tgctccatga gttgcttttc
    44221 cttctactgt tatttcaaat atatccgcag atgccatagc tggaccacgt gaataagcta
    44281 cttgacctgt gttaagaaat ttacccacca tactgtgaaa tgccatagct gcatcgacct
    44341 gaggattttc taatatacca tcatcaatca ttgattttgc acctctggta gtctcttcat
    44401 ctggttcaaa tacaaacttt actctacctt taagtaagtg cttattatcc ataagtattt
    44461 tagctgctcc taagagcatt gctgtgtgat tatcgtgtcc acaactatga gatgcatatt
    44521 tattcttact tttaaaaggt aaatccgtct cctccataat aggaagtgca tccatatctg
    44581 cccttaataa taaggtcttt ccaccatttt cactgtctaa aacagctact attccattat
    44641 tagacgtcaa tttcggagaa tatcccattt tttctaaagt actggtaaca aaggttactg
    44701 ttttcggtag attgcaacca atttcgggat tttcatgtaa atatcttctc catctaataa
    44761 tttccccttc tattgactgc gcttctttaa aaaaattttt catattcctt cctcctaatc
    44821 agatagtcag caaaatctca ctttctttta gtaatgaaat gatgctgata taaaaattat
    44881 aattttatat attaaatttc atgtcttttt aaggagttgg aatatcatct ggaataggac
    44941 atttatatgt tttaccattc atagatttat caaattcttc ttttgccttt ttagctatct
    45001 ctggattatt taatatttta gctccaaaca tagccataat ctttgatgca tatatcatac
    45061 ctttttctcc tatagaactt cctgcacaag atgtaaactg ccagctatga cctggtgctc
    45121 ctagattcgt acaccctgta aaaaagtatg ctgtcggaac tatatgcatt acatctccaa
    45181 tatcagtaga gccataacta ttaaagtttg taacatttcc tccaccataa tatagatgag
    45241 ttccttctgg caaattatat ttacttatca tagccttata ttgttgtggg ctttgtctat
    45301 ctaattcagc tgcaaaatcc aattcctctc gagtccaagg ctcttgttta atctcattca
    45361 tacattctga tacaagattt gctaaaacat gattgtttaa agtattataa catcctccta
    45421 aaaattctac ttcaacttca gtttcagtca tcattgcagc tccttttgct acctttacaa
    45481 gtctttcata tgtactttct acagcttctc tacttaaagc tcttgtataa taccatactg
    45541 atgctttatc aggaactatg ttgggtgcta ctcctccatc tgtaattgta taatgaatcc
    45601 ttacatcaga agtaacatgt tctctcaaat aattagctcc cacattagta agttccacag
    45661 catctagtgc acttcttcca ttttgagggt ctgagcctgc atgtgcagtc tttcccttaa
    45721 aatgaaattt tacagaatta acagctgttg aagtaccaat tgtagcttca ctaattttat
    45781 ttggatgaaa atttatagct aagtctaacc cttcaaatgc tccacctcta gccataaatc
    45841 ctttacctgt taattgttct tctcctggac atgcataaaa tactatagtc ccacttaagt
    45901 tattttctag catctctctt ttaagtccta ctactgcccc tacatgtgct gtacataata
    45961 aattatgccc acatccatga ccatatgcac ccaattcaaa tgcttcttgc tttgttgaaa
    46021 ctttttgatt taaaccaggt aatgcatcaa attctcccat aaaacctata actggtttac
    46081 ctgaaccaaa actagctcta atagccgtgg ctaaacctcc tacacctact tccacatcaa
    46141 atccttcact tcttagaaaa tttgctgtat tttcacatgc tttaaattct ttaaatgctt
    46201 tttctgggtt ttcccatatt ttcttactta aatcatcaag tttgaatctt tttttatcta
    46261 tctcatttat agatatactt cctatcattt cctattcctc ctataagtat atttactatc
    46321 aaccaaatat aattggacct attatactag caaatgccac tgatgcaatg gttacagtaa
    46381 caaatcctcc tataatcatc tttggtaata aataatctgt tgccctttgt ttttcttctt
    46441 cagttccttc catagcatca ataccttcgt tggaaattat ctgtgttgca ggataagcta
    46501 gcatacaagt tacaccaatt gcagctgaca ttcttggaga atatcccaat agttttccta
    46561 caattgcagt aaatagtatt ataaatacag agcctacaac aagtattccc atagttggaa
    46621 caatcatccc aaacaatgct gttggagtta ccttagcaag tcctccaggt aacatcaaca
    46681 tacaaccaaa cattataatt ccattagatt gagatttatc taaaattcct ttttctaaaa
    46741 aacctattcg tgtaaaaatc aaccctacta acaaacatgc tatgttggga tttaaataat
    46801 agtttgcagg ggtaaatcct ggtattaaag tagccaatcc tacaaaatta ccaatcatag
    46861 caacaaatga aagctttgca agatacacta aagtagatga ttttttacct aaatcttctt
    46921 taaaaattct catacttgga agtttaaaat tactttttga ctcatctttc aaaaaatccc
    46981 cattatctct tttaaatttt aattctttat ttatacaata tgtagttatc ggaataccaa
    47041 agaacttttg aaatgataat actagaatta caaatgcagc aagttcaggt ctatttgcag
    47101 cttctgcaac agttgttacg ataatcccag caacagtaga acctgctatt ggaggtgctg
    47161 ctattaaagc atactctctg ccaaataaca tactacccac tgttaaacag ccaacaccta
    47221 ttcctacaat acctactaat gcaattacaa ccgttttcca ttcgtgacat aagtcttcaa
    47281 gatttataag tgttcctaag ttagttatta ataaagctat accaaagttg ctcattgcag
    47341 ctaatatccc tgatgctcct gtaatgtctt ttggtaaaat atttgtccaa aatcctagta
    47401 aaaatataat acatacaaat aacaatgttg aaattgcacc ttttgttttc ttagataaaa
    47461 actcactaaa agcataaatc attacacata cagtaaagca agtaagtgga ttccaaaaat
    47521 tttccattgt tgtttcctcc taaatataat aattttttat caataaaaca ataattataa
    47581 aatgtatatt atgcagttat tcttaactac atatggtata gcttgatatt ttttgatgat
    47641 attatatacg tcctaaatta ttcctaaaat atctaaataa atatagctag agttaactct
    47701 ctaaatatta actatagtta tcaacttact agatttcttt ttgattttta ctttctacat
    47761 aattttacga gttagaattg tacttcaact actctctata ttagtataca tgttggaaga
    47821 cttatattta tttaaatatt agatatagaa tacattttta taaacgtctt aaattattct
    47881 taaaatatct aaaacgaaat aataatatac ccattaaacc aatattatca gaatatactt
    47941 cactttaagt cttctttata tagaaatttt atctattagt gcatcccctc ctttgtaaat
    48001 tagactattt ctaaacgaat ttaaatatct tattcctagg aactattact aaaaacaaaa
    48061 attatttatt atattaaatc aatgataagc catactttaa aattctttct atacaa
//
LOCUS       DBGJFF010000013        47967 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.013, whole genome shotgun sequence.
ACCESSION   DBGJFF010000013 DBGJFF010000000
VERSION     DBGJFF010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 47967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47967
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.013"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(382..558)
                     /locus_tag="ACOIAF_000817"
     CDS             complement(382..558)
                     /locus_tag="ACOIAF_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667843.1"
                     /translation="MFFTFIGVISAILLLCTIICGLWIKLNNRNNDSNSHFTFSILSI
                     AFSLLIVVAFLVRN"
     gene            complement(662..1243)
                     /locus_tag="ACOIAF_000818"
     CDS             complement(662..1243)
                     /locus_tag="ACOIAF_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HGZ2667844.1"
                     /translation="MKADSTKEQLINVTIQLLSSHRDVSSITAREIVAKANVNLAMIN
                     YYFKSKEELINISIKKFLKRHMDEYKATHDTKNLAPKQQLREILIAFFDFIGEHSQFI
                     KISVPYILIQEEIVYPFEILPLIKAHYENQNNKKTEAEYRTIAYQITSFIQLLFLRSN
                     AFFKYSGIDILNIEQRNGLIDFQLDLLLVDITK"
     gene            complement(1369..2193)
                     /locus_tag="ACOIAF_000819"
     CDS             complement(1369..2193)
                     /locus_tag="ACOIAF_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HGZ2667845.1"
                     /translation="MFKIGEVSKLTQISIRMLRYYDELGILKPAKTDKYTGHRLYSVE
                     QISILQRIVLLRDSKFSVAEIANIVHNWNDEFVIKELNRKKNEIQKEIKQEQQRINKI
                     DKFIEAINCDKDEIHYNVVFKKIPSYKIISLREIIPDYQSEGILWEKLSKFIKEEHIE
                     VSRQSNNNIAFYHDEEVKDNGVDIEVGMVVKKIGKNKSGFIYRETEEIDMMACTMVYG
                     PYENIAGAYESFCYWLDKNSDYQISGINRQIGHKGEHNEINPENYLTEIQIPLIKV"
     gene            complement(2371..3264)
                     /locus_tag="ACOIAF_000820"
     CDS             complement(2371..3264)
                     /locus_tag="ACOIAF_000820"
                     /EC_number="3.4.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HGZ2667846.1"
                     /translation="MQLYNTPFNTNKHLIYFFTFTLVLNWIFSLTPALLGITNTILGE
                     IIFYLAYTSPAIVGLFFISTIYPQSAKQDFIYRYLSFKEIRIKWILLTILFFALTFIT
                     SLIIGNYFNVKTSDIDWIHIAVFRPSKIAYMLLASFILSILSQESGWRGYAIDKLLVR
                     FGFIGSSIILGLVCGIWYLGSYFTPNQIPYNLAQYSLFDAFLFIPNIVLLNFIINFVY
                     INTSRSILSAGLVHMMYYFFNIQLLLYYPIKLCVIAQYVQIIFGLIFLVYMISSNKFK
                     QKVNSEIEQIKSDDFEFELDW"
     gene            complement(3521..3958)
                     /locus_tag="ACOIAF_000821"
     CDS             complement(3521..3958)
                     /locus_tag="ACOIAF_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HGZ2667847.1"
                     /translation="MKIHVEQGINCDDIEITIKCSALDNNIEKIISLLSTPLLDIKGK
                     KSGEIYKLNVCDILYFETVGNKTFAYYEDDVFEIDLKIYKLSKLLANTSFSRISKSMI
                     LNMDYIKSIKVISHNRMQATLKNNEKVIINRRYIKNIKEKLNI"
     gene            complement(4215..4364)
                     /locus_tag="ACOIAF_000822"
     CDS             complement(4215..4364)
                     /locus_tag="ACOIAF_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvrJ family protein"
                     /protein_id="HGZ2667848.1"
                     /translation="MDDSLQLLITNVGFPIALSMYLLVRIEGKLNTLSNSIDTLSKNI
                     LIFKK"
     gene            complement(4377..4607)
                     /locus_tag="ACOIAF_000823"
     CDS             complement(4377..4607)
                     /locus_tag="ACOIAF_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HGZ2667849.1"
                     /translation="MEIKKKLVMSFKNVKDKQVSFSIENPKDDLTEENIKSVMDLIVS
                     KNIFSVGGFDLASVVEAKVVETNTTPYDLVIE"
     gene            complement(5179..6372)
                     /locus_tag="ACOIAF_000824"
     CDS             complement(5179..6372)
                     /locus_tag="ACOIAF_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005343085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGZ2667850.1"
                     /translation="MSEKRRDNKNRILRTGESQRKDGRYAYKYIDTFGKPQFVYSWKL
                     VPTDKTPAGKRDDIALREKEKEIQKDLDDGIDHIGKKMTVCQLYAKQIRHRANVRHGT
                     KQGRKQLMRILQEDKLGACRIENVKLSDAKEWALRMKEKGYGFKTINNHKRSLKAAFY
                     TAIQDDCIRKNPFDFQLNTVLEDDTEPKEPLSPTQEAAFLSFVQHDKVYQKYYDEIII
                     LLGTGLRISELCGLTEADIDLDKQLINVDHQLLKIADVGYYVETPKTKSGNRIIPMSE
                     KVLEAFQRVLNKRKYTQPVILEGYTKFLFLNRNGLPKVAVNYESMFRGLVRKYNKTQK
                     VALPKVMTPHTLRHTFCTTLANAGMNPKALQYIMGHSNINMTLNYYAHTTSETSITEM
                     KRLIA"
     gene            complement(6452..6655)
                     /locus_tag="ACOIAF_000825"
     CDS             complement(6452..6655)
                     /locus_tag="ACOIAF_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HGZ2667851.1"
                     /translation="MNNNDVPIWEKYTLTIEEAAKYFRIGESKLRKLAEENPVPDWVM
                     MNGNRIQIKRKQFEKMIDTVDAI"
     gene            complement(7077..7334)
                     /locus_tag="ACOIAF_000826"
     CDS             complement(7077..7334)
                     /locus_tag="ACOIAF_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGZ2667852.1"
                     /translation="MNGTNGNQNDYPENALVPYPVILAASKGDPDAMKMVLQHFSGYI
                     ASLSMRKLYDERGNVYYGVDEDIRERLQARLMRAILTFRAE"
     gene            complement(7315..7755)
                     /locus_tag="ACOIAF_000827"
     CDS             complement(7315..7755)
                     /locus_tag="ACOIAF_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009255547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HGZ2667853.1"
                     /translation="MEPNRREFQKQCAFQSFCKRVLHNEACNAHDEIRRRRKREVSFC
                     DLALHEERQLYTTDKYFQAETAEPTYQMAGKEITPKLLLEAIRTLPEEKRTAILLYYF
                     EGMTDVEIGKMFNTSRSTIQYRRTSSFELLKKYLEEHADEWNEW"
     gene            complement(8228..9001)
                     /locus_tag="ACOIAF_000828"
     CDS             complement(8228..9001)
                     /locus_tag="ACOIAF_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2667854.1"
                     /translation="MRNIIKTEFLKLKRYSVIKAGIIMTTLSPLLSLFYSTANGGQTW
                     TFDYFMQQVMISNCTLFFPIIIALIAGYIITREYTDDTMKNILTIPIPYKQLLSGKLL
                     ILLLLTISFSLIGCVIALVINIIVGFPGVHFGNLLNMFIRVTGANIGIYISVLPIILI
                     FCCSANNFLGGVALAFVYGYFGTFEGTLLNYYPIKASMILVDPTCGTEYGYTYHIFPA
                     FITIVLTFLISMIILANKKKEPGTLTVGKKKKAVRKKGW"
     gene            complement(9007..9816)
                     /locus_tag="ACOIAF_000829"
     CDS             complement(9007..9816)
                     /locus_tag="ACOIAF_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2667855.1"
                     /translation="MLKLIKCEFWKLKRKRLLYALMSLSLFFPLILAYMAKAGTGADT
                     TEHYLQTRFDYVYTMMLGYGLVFLLPCLIGIIAAILFFIERDCDTSKNLRTIPVTNTQ
                     LIMAKISMLFIFSVAFCLISTLSVALFCKLFHVGMVYGMTYKIFMSLIFGVLIVAASL
                     PIVFLIIYFNKSFLLSILLAFFYSIFNWGILGTIGTSISAAKIAFLNYFPVICVMNWT
                     SGLMMDNLQKDNLLPEAYAIVPTTCHTIFIMAITVILSLWLIIRFYKKWTR"
     gene            complement(9809..10735)
                     /locus_tag="ACOIAF_000830"
     CDS             complement(9809..10735)
                     /locus_tag="ACOIAF_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2667856.1"
                     /translation="MDKNYIIETKNLTKQYGSQKSVADLNIHVQKGRIYGLLGRNGAG
                     KTTTMKMLLGLTKPTSGEVKIWGKSLQGNEKKLLPRIGSLIESPGFYPNLTATENLQI
                     FATLRGIPNRHSIKNTLDLVGLPYKDKKLFSQYSLGMKQRLAIALAVMHDPELLILDE
                     PINGLDPIGIAEVRSFIRELCDTKGKTILISSHILSEISLLADDIGIIDHGTLLEEES
                     LAELEQKSSKHIRFTLSDTAQAARILERTFHESHFSIQDDHNLRLHNLDLSVGKIVTA
                     FVENGLEVSEAHTCEESLEDYFKRVTGGEGIA"
     gene            complement(10872..11789)
                     /locus_tag="ACOIAF_000831"
     CDS             complement(10872..11789)
                     /locus_tag="ACOIAF_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HGZ2667857.1"
                     /translation="MEAVIFLSIIIALFSILVSCFAIRRVKKQIAEITDALIDVKNGN
                     GNRRILSATNELVAPLAYEINEIVVSYESRLSTVRQTEETNRQLMTSLSHDVRTPLTT
                     LIGYLDAAHKGIVTGKDRDDYIETARRKAHDLKEYIDVLFDWFKLNSNEFAMDINIVE
                     AAELTRNILIDWIPIFEDKQIDYNIDIPEQPFRVKLDTDGYMRILNNLIQNVISHSHA
                     DKIEIILSKQEKNMQIRLADNGIGIEKEDLKHIFERLYKCDKGRSEKGSGLGLSIAHQ
                     LVEKMNGTITANSIQGTGTEFTLLFPLAN"
     gene            complement(11796..12488)
                     /locus_tag="ACOIAF_000832"
     CDS             complement(11796..12488)
                     /locus_tag="ACOIAF_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGZ2667858.1"
                     /translation="MNKVLIIDDDKELCALIKRSVLSEDIEADFCNTGKEGLQKLKER
                     EYQLVILDIMMPGMDGFETLEEIRKENSLPILMFTSKNDSASKVRGLRAGADDYLTKP
                     FDMDELIARIISLIRRYTRFNQQDGISQQLEFDGLKIDIENRSVTTENGTFELPPKEF
                     DLLLYCAKNQGKILTKQQIYEEVWREEYFYDDSNIMAIISRLRKKLEVNPGSPKYIQT
                     IKGIGYRFNKEV"
     gene            complement(12481..12669)
                     /locus_tag="ACOIAF_000833"
     CDS             complement(12481..12669)
                     /locus_tag="ACOIAF_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGZ2667859.1"
                     /translation="MEYMSCPEAAKKWGISERRVQILCREKRIQGVSKLGYMWLIPKD
                     AEKPIDGRTKQGKELYHE"
     gene            12831..13184
                     /locus_tag="ACOIAF_000834"
     CDS             12831..13184
                     /locus_tag="ACOIAF_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGZ2667860.1"
                     /translation="MAKRPVEKYDFKAFGEAIKAARTGRKESRKQVSDEMYISPRYLA
                     NIENKGQHPSLQIFFELMLRYNISVDQFLLDTPAPKDTKRRQLDALLDNMSDNGIRIV
                     TATAKEISEVEKEDK"
     gene            13433..13762
                     /gene="mobC"
                     /locus_tag="ACOIAF_000835"
     CDS             13433..13762
                     /gene="mobC"
                     /locus_tag="ACOIAF_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002610348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HGZ2667861.1"
                     /translation="MNGRKRKIQIKFYVTEEERALIEQKMKLVPTRNMAAYLRKIAID
                     GYIIQTDHSDIKAMTAEIQKIGVNINQIAKRVNATGSVYQEDIEEIKGVLAEIWRLQR
                     LSLLKAR"
     gene            13723..15072
                     /locus_tag="ACOIAF_000836"
     CDS             13723..15072
                     /locus_tag="ACOIAF_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005928627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HGZ2667862.1"
                     /translation="MAVTKIKPIKSTLSKALDYIQNPDKTDGKMLVSSFGCSYETADI
                     EFGFTLAQAIEKGNNLAHHLIQSFEPGEVDYEKAHEIGKQLADAVTKGQHEYVLTTHI
                     DKGHIHNHIIFCAVNFVDYHKYNSNKRSYYGIRNMSDKLCRENGLSVVVPGKGSKGKS
                     YAEYQAEKVGASWKGKLKITVDTLIPQVASFEELLQRLQAAGYEIKPGKYISCRAPGQ
                     ERFTRLKTLGADYTEEAIKERIEGKRTKAAKAPKEQRGVSLLIDIENSIKAAQSRGYE
                     QWAKIHNLKLAAKTMNFLTEHKIEQYADLVSRIEEVNAENEKTADALKSVEKRLANMA
                     VLMKHVTTYQKTKPVYEAYRRAKDKDAYRAKQESSLILHEAAAKALKTAGVSKLPNVA
                     ALQVDYEKLREQKEALYADYGKLKKQVKEYDVIKQNIDSILRQPREPEREKGKERGE"
     gene            15165..15395
                     /locus_tag="ACOIAF_000837"
     CDS             15165..15395
                     /locus_tag="ACOIAF_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGZ2667863.1"
                     /translation="MEQEMPDYETICAAVVGEKWALEKVLDCYGDEINRLAMVKKCQP
                     DGTIKEEIDEDLRQTLIMKLLEAIPQFPMEKE"
     gene            15400..15786
                     /locus_tag="ACOIAF_000838"
                     /pseudo
     CDS             15400..15786
                     /locus_tag="ACOIAF_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728512.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6050 family protein"
     gene            15779..16198
                     /locus_tag="ACOIAF_000839"
     CDS             15779..16198
                     /locus_tag="ACOIAF_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HGZ2667864.1"
                     /translation="MNERERIIHLWFDMWLKQQDLGIDSIFTEDVIYTESWSPKYESR
                     KTVKHWFDEWNTRGKVLVWDIKQFFHKENQTIVEWYFKNKMNNGNIEEFDGISLIVWT
                     ENNKIMELKEFGCNLNTYNPYAQNDMPQFRDEKTKWF"
     gene            complement(16376..16741)
                     /locus_tag="ACOIAF_000840"
     CDS             complement(16376..16741)
                     /locus_tag="ACOIAF_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HGZ2667865.1"
                     /translation="MTGLKRLTPPQSRKVNSLVKKECCNCDNGHCILLDDGEECVCPQ
                     LISYSLLCKWFQTAVLPLDKLLYAELFKTEDKKRCTECGASFASTSNNVKYCPDCRKR
                     ITRRQAAERMRKRRALVTQ"
     gene            complement(16738..16944)
                     /locus_tag="ACOIAF_000841"
     CDS             complement(16738..16944)
                     /locus_tag="ACOIAF_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HGZ2667866.1"
                     /translation="MIRLTVEETNLLSIYNEGGKRGLMENINAALPFMDEDMRELAKR
                     TLAKIAPLTENEYAELAIFAADEV"
     gene            complement(16947..20978)
                     /locus_tag="ACOIAF_000842"
     CDS             complement(16947..20978)
                     /locus_tag="ACOIAF_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008727275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HGZ2667867.1"
                     /translation="MASLRDTVKDYQEELRDGIAWVAFWKTGRSWNAEHFSLEMGDIL
                     YPEDRSRMEEIKQADPAAVVVNGYYSGYLGEDMSLDELTAGVRRHYENGYSNIGEFIE
                     AHDNRLPPELIEEARAAAHAAGLPFSEKAYRDGEEPDPYIFDGSMSMEDYELMHRMIE
                     NERSERMEETILSGYLSNLGKYTEGRPAGEWVSFPTTAEHLKEAFDRIGIDFKNYGEW
                     YFTEFQSSVPGLTTHLNEFSHPDELNYLSELLKMQFDDDREKFVAAMEYGDHTRDLQD
                     IINLAQNLDCYWLYPSIQCEEDYGHYLIEELDELELPEEAKKYFMYEEYGRDAAINDG
                     GSFTEQGYIYNNRNTFTEWYDGCEVPEEYRVTPQPPVQEKEQADLDTSVAIQTTATEQ
                     PPVLPIILSSEKPADKMKEITDRLEQGILGLYESDRYADYLRTMSKFHDYSLNNTILI
                     AMQGGNLVKGYKQWEKEFDRHVKPGEKAIKILAPSPFTVKKQVEKIDPDTQKPVFDKD
                     GKPVTEEREIKIPAFRVVSVFDISQTEGKELPALTYELTGNVEQYKDFFAALEKTFPF
                     AMGFEALSGGVKGRCNYEEKRIIINEGMDELQNIKTAIHEIAHATLHDTALAMPERPD
                     RRTREVQAESVAYAVCQHYGLDTSDYSFGYIAGWSSGKELAELKGSLETIRSTAASLI
                     DTIDGHFAEIQKTQNKEQTTEQAQPAQETAKQPETEIAAPELPEEKAPAKEQDGQPEQ
                     TTQTAAPYYTINEAAAKRAKDMNSFSDYKQGSATAEYRHYVDEAVQIAERQKQRVDPM
                     YHEKIDSLLDTYARKLAANMNKGYEIDARVPSILIAGGSNFPTRKKEKQNAARDSNYR
                     EWQDIQGLLDKIRSTGMGGISADDPQAVQKLEKKLESLEKSQETMKAVNAYYRKHKTL
                     DGCPHLPPEELEKLKADMASSWHLEDKPFATWALSNNSAEIRRVKDRIKSLSQQKEIG
                     FVGWEFDGGKVEANTEANRLQIFFEDKLDETTREALKSNGFRWSPKAGAWQRQLTSNA
                     YYAADYVKAIAPLTGEKPTELQWAHIRAQKVAAQEQPAQEQPEQEAPQDKDTFSIYQI
                     KGEEETRDLRFEPYDRLTATGHRVDPKNYALVYSAELTPGTSLEDIYTRFNIDHPKDF
                     KGHSLSVSDVVVLHQNGQDTAHYVDSFGYKEVLEFFKEQEKALIPDDLETGETIKTPR
                     GTFYVTAMSREQMEAAGYGLHHQSDDGKYLIMGNGTRAFAVPVQPENYLKAAEQTTEQ
                     NYNMIDGQINNTPTTAELEAKVKAGETISLVDLAEAVKADKERGKAAKTEKKPSIRAQ
                     LRADKEKTAQKKAAKSKNHELEV"
     gene            complement(21111..23201)
                     /locus_tag="ACOIAF_000843"
     CDS             complement(21111..23201)
                     /locus_tag="ACOIAF_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HGZ2667868.1"
                     /translation="MILVIAEKPSVAQTIAAVLGVKEKKDGFLTGSGYIVSWCVGHLV
                     GLAEAAAYGEQYKKWNYDSLPILPQEWKYTVAADKEKQFKTLKELMHRADVSEVVNAC
                     DAGREGELIFRFVYEMAGCKKPMRRLWISSMEDSAIKEGFSRLKNGKEYDALFASALC
                     RAKADWLIGINATRLFSVLYNHTLNVGRVQTPTLKMLVDRDAAITTFKKEKYYHVRLA
                     LSGAEAASERISNKAEADTLKTTCEASKAVCTSLLKEKKTAAPPKLFDLTSLQREANR
                     LFGYTAKQTLDLAQALYEKRLLTYPRTDSSYLTDDMGDTAAGIIKLLCGKFPFMAGKD
                     FTPELAKVLNSKKVSDHHAIIPTMELAKTDLAALPESERNILTLAGARLIMATAAPHT
                     FEAVTAVFECAGQSFTARGKTVLSDGWKEIDRKYRAILKSKPETDDADSDTEKTLPPF
                     TEGQNFDNPAAKVTEHDTTPPKPHNEASLLSAMERAGSEDTDPDAERKGLGTPATRAA
                     VIEKLVKGGFVERKGKQLLPTKDGINLVCVLPDTLTSPQLTAEWENNLTQIAKGKADP
                     AAFMKGIEDMARELVKTYPFLSEADKGRFKEEKPELGKCPRCGSPVYEGKKNYYCSNK
                     ECSFTMWKNDRFFEERKVTFTPKIAAALLKSGKVNVKKLYSPKTGKTYDGTIALADTG
                     EKYVNYRIELSKKK"
     gene            complement(23218..23406)
                     /locus_tag="ACOIAF_000844"
     CDS             complement(23218..23406)
                     /locus_tag="ACOIAF_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667869.1"
                     /translation="MTLFTDNPFEKMMIQKPNGRRDNAPPVPYPPACASCPYKGQSPC
                     VGYCLKQVQKKKETEPER"
     gene            complement(23403..24131)
                     /locus_tag="ACOIAF_000845"
     CDS             complement(23403..24131)
                     /locus_tag="ACOIAF_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HGZ2667870.1"
                     /translation="MKNKLKKTMTALCAALILIGGFSVPAYAQGAATEQPAGDATNDS
                     NVVVEEKEETPPLTPKGNATLVDDYGGNKQLITVTTKSGNYFYILIDRAAEGEQTVHF
                     LNQVDEADLMALTENGEAAKKPEICNCTEKCEAGKVNTSCLVCVTSMTSCTGKEPTPT
                     PTEQPEEPKEKGGNPGVVLALVLFALLGGGAAFYYFKFMKPKQNVKGDTDLEDFEFED
                     YDEDEPEADTGETPEDEETEEETL"
     gene            complement(24121..24390)
                     /locus_tag="ACOIAF_000846"
     CDS             complement(24121..24390)
                     /locus_tag="ACOIAF_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HGZ2667871.1"
                     /translation="MANNKIERINKEIAKTREKITEYQNKLKGLEAQKTEAENLEIVQ
                     LVRAMRLTPQELNAMLSGGGIPGMNAAPATETADYEEQEENADEE"
     gene            complement(24422..26362)
                     /locus_tag="ACOIAF_000847"
     CDS             complement(24422..26362)
                     /locus_tag="ACOIAF_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002590569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HGZ2667872.1"
                     /translation="MTRDGVVEVNETQQTAERISSREADGDFSQPDSAAAERVIEHLD
                     AAHTRKANKKAVKKEQEATALRTSTSRLQFTDEERATPELEPYIKKSEKAADKLDAAK
                     AAIPKQKKLVKERTFEEATGKAKTRLHFEEQEKPIPGGKKGNPLSRPAQEAGIFVHNK
                     IHSVEKDNSGVEGAHKSEELAERGAKYGARKLKQGYRSHKLKPYREAAKAEKAAFKAN
                     VNFQYHKTLHDNPQLTSNPLSRFMQKQQIKCQYAKAAKKGGAKAAAEATRKTAKKTAE
                     ETRKAAAFAARHPAGVLIAVAALLLFIMISVGLSSCGAMFSGLTNGVLGTSYTSEDSD
                     LVAVENNYAALENGLQSEIDAIESTHPGYDEYRYDLDSIGHNPHELASYLTALLQSYT
                     PQSAQAELERIFEKQYTLTLTEEVEIRYRTETSTDPETGETTTEEVPYEYYILNVKLE
                     NKPISSFVSELLTPQQLEMFHVYLETSGNKPLIFGGGSPDGSPSEDLSGVEFVNGTRP
                     GNQELMELAKQQVGNVGGYPYWSWYGFNSRVEWCACFVSWCYNQAGKSEPRFAGCEWQ
                     GVPWFQSRGQWGARGYENIAPGDAIFFDWDLDGVADHVGLVLGRDGSRVYTVEGNSGD
                     ACKIKSYDLNYQCIKGYGLMNW"
     gene            complement(26389..27348)
                     /locus_tag="ACOIAF_000848"
     CDS             complement(26389..27348)
                     /locus_tag="ACOIAF_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HGZ2667873.1"
                     /translation="MKTDVIINRDALAALKELPSESVHCCVTSPPYYALRDYGLDAQI
                     GQEDTPKQYIDRLTAVFGELYRVLRKDGTLWLNIADTYCSTGNKGGYADPKNPKGRTG
                     QRVARNSRVTGCKQKDLIGIPWLLAFSLREQGWYLRSDIIWQKQNPMPESCKDRPTRC
                     YEHIFLLSKEKKYYYDAAAIAEPIAPTTAERYRRARSTNSKYTQEISGQGKVQGLNRP
                     RDGGYYDDALMPTTRNKRDVWLINTVPYKGAHFAAFPPKLAETCILAGCPKGGIVIDP
                     FFGSGTTGFAAKSLDRHYIGIELNAEYCALARVRIGGAGLSSN"
     gene            complement(27345..29774)
                     /locus_tag="ACOIAF_000849"
     CDS             complement(27345..29774)
                     /locus_tag="ACOIAF_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4-like conjugal transfer ATPase, CD1110
                     family"
                     /protein_id="HGZ2667874.1"
                     /translation="MSKGQTKKTREAAGNRRKLTRAEKKQIAEAIRKAKGDGKARTAQ
                     QTIPYLAMYPDGICKVTEKRYSKCVMFEDINYQLAQADDKTAIFENWCDFLNYFDASV
                     SVQLSFINQGTQREQAEKAISIPAQEDAFNSIRTEYSDMLKNQLSKGNNGLVKHKYIT
                     FTVEADNKAAAKSRLSRIETDVLNNFKVLGVTARPLSGYEHLKVLHGVFHPEGEPFSF
                     SFDWLTPSGLTTKDFIAPSSFHFGEGRYFRMGKKIGAASFLEILAPELNDRMLADILD
                     LETGVIVNLHIRSIDQSEAIKTIKRKITDLDKMKIEEQKKAVRSGYDMDIIPSDLATF
                     GSEAKNLLQDLQSRNERMFLLTFLVVNMADTKRKLENDIFAAAGIAQKYNCRLTRLDY
                     QQEAGLLSSVPIGENLIPIQRGLTTSSTAIFIPFITQELFQTGQALYYGLNALSNNMI
                     LCDRKQLKNPNGLILGTPGSGKSFAAKREMTNAFLITDDDIIICDPEAEYFSLVQRLN
                     GQVIRLSPTGRGIDGKPQYVNPMDINLNYSEDDNLLALKSDFILSLCELVIGGKEGLQ
                     PVDKTVIDRAVRNVYRPFLADPDPAKMPILGDLYDELLRQPEPEAARIAAALELYVSG
                     SLNVFNHRTNVELSNRLVCFDIKQLGKQLKKLGMLIVQDQTWNRVTINRAEKKSTRYY
                     MDEFHLLLKEEQTAAYSVEIWKRFRKWGGIPTAITQNVKDLLASREVENIFENSDFVL
                     MLNQAQGDRTILAKQLNISPQQMKYVIHTEAGEGLIFYGNVVLPFVDRFPKDTELYRV
                     MTTKPEEVSSL"
     gene            complement(29722..30126)
                     /locus_tag="ACOIAF_000850"
     CDS             complement(29722..30126)
                     /locus_tag="ACOIAF_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HGZ2667875.1"
                     /translation="MAYVPVPKDLSKVKTKVAFNLTKRQIVCFAAALLIGLPLFFLLK
                     GSAGTNLAAMAMIVVMLPCFLLAMYEKHGQPLEVVVRNIIQTKFTRPKERPYRTENLY
                     AVLEKQRKLEKEVSAIVKRTNKKDAGSRRKQA"
     gene            complement(30148..31017)
                     /locus_tag="ACOIAF_000851"
     CDS             complement(30148..31017)
                     /locus_tag="ACOIAF_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002595174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB6/TrbL-like conjugal transfer protein,
                     CD1112 family"
                     /protein_id="HGZ2667876.1"
                     /translation="MQSILDAINEWIKEILIGAINGNLSTMFGDVNEKVGTIANEVGQ
                     TPQGWNANIFSMIQTLSENVIVPIAGLVITYVLCYELISMVTEKNNMHDIETFMFFKW
                     IFKAFVAVFIVTNTFNITMAVFDMAQHIVSGAAGVIGGDTNIDVAEALAAMQEGLEDM
                     EIPELLLLVLETSLVSLCMKIMSVLITVILYGRMIEIYLYCSVSPIPFATMTNREWGQ
                     IGNNYLKGLFALGFQGFLIMICVGIYAVLVNDMIIADNLHSAVFSLAAYTVILCFSLF
                     KSGTLAKSIFNAH"
     gene            complement(31034..31117)
                     /locus_tag="ACOIAF_000852"
                     /pseudo
     CDS             complement(31034..31117)
                     /locus_tag="ACOIAF_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147847.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family mobile element protein"
     gene            complement(31191..32843)
                     /locus_tag="ACOIAF_000853"
     CDS             complement(31191..32843)
                     /locus_tag="ACOIAF_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009249964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HGZ2667877.1"
                     /translation="MNPNTVYAEHYPLEVRAAVYCRLSKDDDQDGTSASIQNQKELLH
                     RYCEEQGWRVAATFEDDGYTGLNMERPGLKRLLAGVEGGRFDVILTKDLSRLGRNYLQ
                     TGQLLEEFFPRNHVRYIALNDAVDTDIENDITPFRNILNEMYSRDVSKKVHSSYLTKA
                     KSGKFTGCLAPFGYRKDPEDKNHLLPDEETAWIVRKIYDYARNGHGPNYIRRRLEDEE
                     IPAPVWWNRKKGLRDKRSKFEIADPEHGRFIWDFTTIKEILSNPVYIGVIASQKVVYR
                     FKVGWLSDKKREDWIAVEGMHEPLIDRETYDIVQQKIKDRKRPDAWGNFSLFAGLVKC
                     GQCGSALNIRTANAKSKEKIFTCSKYNKYGVAHCSQHRISYDALYSIVLEQIRECARA
                     ALADEQDAADQLKESCRAEEESERAAILQSVAEDEERIATLERVIGRVYEDMAEGRIT
                     EDNFNRILERSQGEQAVLKNRVTLNRQRLDEQAREQEDNTRWLEIIKDYADVQELDAV
                     TLNRLIQKIVIHEDKDGSTVRQTVEIHFNFKKQADKRRLDRE"
     gene            complement(32833..33042)
                     /locus_tag="ACOIAF_000854"
     CDS             complement(32833..33042)
                     /locus_tag="ACOIAF_000854"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667878.1"
                     /translation="MAKPKDPVITVSAVFAGKQSDRQAFIDLILQKQEIVKSQSIIDN
                     SKPMEYNEDTPKRGIHSGMEDINEP"
     gene            complement(33055..33894)
                     /locus_tag="ACOIAF_000855"
     CDS             complement(33055..33894)
                     /locus_tag="ACOIAF_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGZ2667879.1"
                     /translation="MRDELERMMDGIAAAATPAPEDYTDPATGLLFCGKCHTAKQCRV
                     TLFERERLMPCLCQCEQERREAAEAARREQERLDRIRRLKATALQDRALLAYTFAGDD
                     GQNPAMKYARRYVEHWAAMKERGQGLLLWGGVGTGKTFAAACIAHVLTEHAVPVLMTN
                     FSKILNSLSGMFSENRNKYLASFHHFSLLIIDDLGIERNSEYALEQVYNVVDSRYLSR
                     LPFIITTNLPLAELQAPKDLAHARIYDRVLERCTPICFNGPNYRKGNAAANKDEASRL
                     LND"
     gene            complement(33891..34634)
                     /locus_tag="ACOIAF_000856"
     CDS             complement(33891..34634)
                     /locus_tag="ACOIAF_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HGZ2667880.1"
                     /translation="MSDNRKYYYLKLKESYFDEDSIVLLESMQDGVLYSNILLKLYLK
                     SLKHGGRLQLDENIPYTAQMIATITRQQIGTVERALQIFLKLGLVELLDGGAYYMSNI
                     ELLIGQSSTEGERKRAARQKNRALLPSRTNGGHLSDVRPPEIEIELEIEKEIERELKK
                     GQPARTYGRYQNVLLTEAELADLQAEFPTVWEKYVEKLSAYMESTGRKYKSHAATIRR
                     WASEDSQKAASGTKGGGLPDYTYKEGESL"
     gene            complement(34749..35144)
                     /locus_tag="ACOIAF_000857"
     CDS             complement(34749..35144)
                     /locus_tag="ACOIAF_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HGZ2667881.1"
                     /translation="MNRQREAIPIMDYRQYRRARRLVHNCCNYDGGNCLALDDGEECI
                     CVQSISYSLLCRWFRAAVLPLDRELETALLYRAEAKRCAVCGAMFRPASNRAKYCPEC
                     AGRMKRIGAAQRKRKQRLKCHALGIEKGP"
     gene            complement(35128..35262)
                     /locus_tag="ACOIAF_000858"
     CDS             complement(35128..35262)
                     /locus_tag="ACOIAF_000858"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667882.1"
                     /translation="MKETILNITITMTIRLPLPPTPPAPAAAAMRPEEQERPADEQAA
                     "
     gene            complement(35259..36731)
                     /gene="mobQ"
                     /locus_tag="ACOIAF_000859"
     CDS             complement(35259..36731)
                     /gene="mobQ"
                     /locus_tag="ACOIAF_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="HGZ2667883.1"
                     /translation="MRPDYPPPERRRATIAIYHCTIKIIKRSEGRSAVAAAAYRSGQK
                     LTNEWDGQTHDYTRKGGVIHSEILLPTHAPPAFHDRSTLWNSVEEIEKSGKAQLAREI
                     EIALPAELDREAQLALVRAYVRDNFVAAGMCADFALHDKGDGNPHAHIMLTMRPLEQS
                     SEWGAKCRKEYDLDPRGQRIPDGKGGWKNHRADTTDWNEKDKAEQWRAAWAEYANRAL
                     EQTGRPERIDHRSYKRQGVEQLPTVHMGVAATRMERRGIATDKGSVNRQIAADNKLLK
                     EIKARITRLYNWTKEKAEGPQGKESVMAQLWQARQEMSQPATRYGKVKALKESAALFN
                     FLQQNGIISMQQLYEKVSAMNGAYYDLRGDIRTTERRLATLTERLEMWVQYEQHKGTR
                     RQLDGMKPGKREKFAERHGAELTLYESAARFLDGLKATGEAVTPKQWRAEADTLTAKK
                     DVLYQEMRAMREEVKAVEQLRKAAEQLAKTEQSRRKELDR"
     gene            complement(36885..37154)
                     /locus_tag="ACOIAF_000860"
     CDS             complement(36885..37154)
                     /locus_tag="ACOIAF_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3847 domain-containing protein"
                     /protein_id="HGZ2667884.1"
                     /translation="MNLLEEKRKIETELHQLQNRQKILLNKKANAERKARTHRLIERG
                     AILESVFPAVAAMTGEEVGAFLLVLSRLPGAGEVAAKSAKTGDAG"
     gene            complement(37151..37618)
                     /locus_tag="ACOIAF_000861"
     CDS             complement(37151..37618)
                     /locus_tag="ACOIAF_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002596397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HGZ2667885.1"
                     /translation="MTVINLKRYYYPLVKTDVFVEVPDEIADALLHLRREENNRTSKI
                     WYHKAYFSLDCEDGIENDAIGWAQPSPEDYMIQAEDEAAQELLLEHLWEAIGQLTPTQ
                     ARRLHMRYMLEMKYREIAEAEGISPTQASDSVRRAVKKLQKYFKKRKWTREGL"
     gene            complement(37700..38128)
                     /locus_tag="ACOIAF_000862"
     CDS             complement(37700..38128)
                     /locus_tag="ACOIAF_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HGZ2667886.1"
                     /translation="MEVTIKINGQALSVEVSVEVYECLDRADHKEENLAHEKRRHWDI
                     REFDEYIVATEGRPPHQETPEEYVCRKETRDELLSVLESCTEAQRRRFLLYALDGLTY
                     EQIARLDGCSKVSVYESIAAVRKKYQKLFKDHPNETRFSG"
     gene            complement(38628..39374)
                     /locus_tag="ACOIAF_000863"
     CDS             complement(38628..39374)
                     /locus_tag="ACOIAF_000863"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HGZ2667887.1"
                     /translation="MKFALITGATSGIGYELAKLFAKNEYGLILVSSSQDHLDNVKKK
                     LQGHYSTPIYVIEQDLTQIGAAYEVYQKVTKEQVPVTVLVNNAGFGLVGATNEIDLKQ
                     DEQMIIINTLNLVILTKLFIAEMYKCGQGKILNVASTGAFQPGPYTSTYFASKAFVLS
                     YSRAIRYEAKSKGVQISTLCPGATRTNFFVREGTVTPTNSMTAEKVAQIAFSGLMKNK
                     EIIIPGLKNRLLQLFPTKIKMISVARMKYK"
     gene            complement(39440..40024)
                     /locus_tag="ACOIAF_000864"
     CDS             complement(39440..40024)
                     /locus_tag="ACOIAF_000864"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HGZ2667888.1"
                     /translation="MIIQPSEYLKNCLKIRLDKDISVVIDNKASVVTYEKGYHPIQMG
                     DEVSSFYFIIYGIVRGYYIDDQGKEATKCFSFENGFFGSECFRTNSCSSFYVECIEEC
                     KCIKLPYTVIRKIISMDKQVEQYVQNLYLEELGKLESRAKYLLLLSADERYISFCKEY
                     PNLQRRIPLKFIASFIGIKAGSMSRIRKKLKNQI"
     gene            complement(40051..40263)
                     /locus_tag="ACOIAF_000865"
     CDS             complement(40051..40263)
                     /locus_tag="ACOIAF_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006573934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGZ2667889.1"
                     /translation="MKISYKPLWKTLIDKNMSKRDLRLQAHLTTNHIANMGKGEHISM
                     ATLVKICETLNCDIADVIALIPDDEP"
     gene            complement(40436..40585)
                     /locus_tag="ACOIAF_000866"
                     /pseudo
     CDS             complement(40436..40585)
                     /locus_tag="ACOIAF_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147847.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family mobile element protein"
     gene            complement(40785..40952)
                     /locus_tag="ACOIAF_000867"
     CDS             complement(40785..40952)
                     /locus_tag="ACOIAF_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667890.1"
                     /translation="MNRRIRKKKDKKITEAINGLVSIAEWQEKQRQAAIRQFVKRCEA
                     LYEQQRKERRN"
     gene            complement(40945..42732)
                     /locus_tag="ACOIAF_000868"
     CDS             complement(40945..42732)
                     /locus_tag="ACOIAF_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015529212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HGZ2667891.1"
                     /translation="MKPELKKLLILNAPYLLFVYLFDKVGQAVRLSPGADLSGKVLSL
                     ADGFSAAFANPLPSFFPADLLIGIVGAVLIRLMVYFKGKNAKKYRKGIEYGSARWGNA
                     EDIKPYTDPVFQNNVLLTQTERLTMNSRPKQPKYARNKNILVIGGSGSGKTRFFVKPN
                     LMQMHSSYVVTDPKGTVLVECGKLLQRGGYRIKVLNTINFKKSMRYNPFAYIRSEKDI
                     LKLVNTLIANTKGDGEKAGEDFWVKSERLFYCALIGYIWYEAPEEEKNFTTLLEMINA
                     SEAREDDPEFQSPVDLMFERLEEKDPEHFAVRQYKKFLLSAGKTRSSILISCGARLAP
                     FDIKELRDLMETDEMELDTIGDRKTALFVIISDTDDTFNFVVSILYTQLFNLLCDKAD
                     DEYGGRLPVHVRCLLDEFANIGQIPKFEKLIATIRSREISASIILQSQSQLKAIYKDN
                     ADTIVGNCDTTLFLGGKEKTTLKETSEILGKETIDSFNTSENRGREVSHGLNYQKLGK
                     QLMTEDEIAVMDGGKCILQLRGVRPFFSDKYDITKHPNYKYLSDYDKKNTFDMEKHLR
                     RRPALVKPDEVFDYYEISESDLQEDTDHE"
     gene            complement(42729..43205)
                     /locus_tag="ACOIAF_000869"
     CDS             complement(42729..43205)
                     /locus_tag="ACOIAF_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HGZ2667892.1"
                     /translation="MQDEVNEKVVSLAIKTSKLTAEVLQKAMKALLAKGKGQLTKAPH
                     GKITMRQLMKPGEKVSNIEITDQNIKAFDPIARKNGLDYNVKKIENGKPPTYLVSFRG
                     KDIDVMTEAFREFTAKKLGRNKKPSIRKLLSTLKDKAAALNAQRDKVKKKDREVSL"
     gene            complement(43288..43506)
                     /locus_tag="ACOIAF_000870"
     CDS             complement(43288..43506)
                     /locus_tag="ACOIAF_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HGZ2667893.1"
                     /translation="MTQKTGALIFDETADRYDIRFDLNDYYGGLHCGDCLEVFVRGKW
                     KPTRMEYGDNWYLVGVRASDLNGLRVRI"
     gene            complement(43527..44501)
                     /locus_tag="ACOIAF_000871"
     CDS             complement(43527..44501)
                     /locus_tag="ACOIAF_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6017 domain-containing protein"
                     /protein_id="HGZ2667894.1"
                     /translation="MAVFRVEKNKGYTVMSNHHLRNKELSLKAKGLLSQMLSLPEDWD
                     YTLAGLSLINRESIDAIRTAVWELEKAGYITRRQGRDEKGKMTAIEYTIYEQPQPPTL
                     DCPVLENPTADKPILENPTPDNPTSENPTQLNKELLKTNLPKKEKLNTDISNTHSIPF
                     HSLNPSPLEDAAQPPERKRKETTDAYRVYEEIIKDNIEYEHFIKHTDFEKERLDEIVS
                     LILETVCTKRKTIRIAGDDYPAELVKAKFMKLNSSHIEFVFACMSENTTKIRNIKQYL
                     KAVLFNAPNTIDSYYTALVNHDMYGGRTIQSARSKGIPDYTYNEGESL"
     gene            complement(45072..45293)
                     /locus_tag="ACOIAF_000872"
     CDS             complement(45072..45293)
                     /locus_tag="ACOIAF_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1659 domain-containing protein"
                     /protein_id="HGZ2667895.1"
                     /translation="MEVVSTKNLSSIKLKLDAGFDDKGKAVVKSKSFANVKAEALNED
                     VYAVAEAIASLQENPIVDILKLDSTSLSK"
     gene            45873..46130
                     /locus_tag="ACOIAF_000873"
     CDS             45873..46130
                     /locus_tag="ACOIAF_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HGZ2667896.1"
                     /translation="MKIKVEDIPVQFHAMVEIIGIDKFIEVAKLYGGTNTYIPTYKGL
                     FRYARNRDIVKQFNGVNHNELAIRYNMCVSNIKRILNENSV"
     gene            46599..47837
                     /locus_tag="ACOIAF_000874"
     CDS             46599..47837
                     /locus_tag="ACOIAF_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HGZ2667897.1"
                     /translation="MNKGFYKLNSVVLNFDIKNDVNKIRFNHYIMRKENIQRLQGTLP
                     NGQFYMTVRRAATDLQLSISTISRLVKEFLDLGIIRLVSKGVKGNRSVYSYVSIEFNG
                     SLFDDSKYMELDCVCNDEFIDNSGIEFDCDSKDNSFNGDINVETVHCINEYFSDDDVE
                     HNYNNFHNKDTIKDKAISNISTIKKYIDESFDTNKSENSNLQQESINFNFNDTCNEID
                     KRTNNSIENNQNKRPMRLSDISVLSDKGMTNKKNYFNHNHGIQKGTKSVTKKKESLNK
                     FNKKNIQQSIINMLNKKSGKNFKIDSPISIKLINERLKEGYVLEDFYKVIEVKVAKWK
                     NTIMEMYIRPETLFSYKFESYVNENISMKSQSNVHPCKEGNSNDSSNYIHSDYTTYDG
                     SVRERKKYENVRYWNPICEI"
BASE COUNT        12259 a        10689 c        10480 g        14539 t
ORIGIN      
        1 caatgtaata gaaatgctgt aataataaag gtctgtagta taataaagaa ctgtaataaa
       61 taagacttta caatataata gaaatgttgc aataacaaaa caccataata aataagagtt
      121 tgcaatataa tagaaacact gcaataaata agagatttca atataataaa agtctgtaat
      181 ataacaaaat cctataataa ataagatttt acaatataat aaaaatccta taataaatag
      241 gactctgtaa tataataaaa tgctgtaaca acaaaacact ataataaata agaccctgta
      301 atataataaa aatactataa tatagtaaaa atcatgcaat aataaaaatc tataataata
      361 aacctcagca taacaaagac attaatttct tactaaaaag gccaccacaa ttaacagtga
      421 aaaagcaatt gaaagtattg aaaaagtaaa gtgagaatta gagtcattat tacgattatt
      481 aagttttatc cataaaccac atataattgt acacaacaat aaaattgctg atattactcc
      541 tataaatgta aaaaacatta aaattcctcc cccttattta atgcaagtat ttttagtcaa
      601 tgagaatatt ttcaaacgtt accataaaat aaaaaaatgt acataaaaaa ttaatatagt
      661 attattttgt aatatctacc aataataaat ctaattgaaa atctattaaa ccatttctct
      721 gttcaatatt aagaatatca ataccactat acttgaaaaa agcattagaa cgaagaaaaa
      781 gcaactgaat aaatgaagtt atttgatacg caatagttct gtattcagct tcagtttttt
      841 tgttattctg attttcatag tgagccttaa ttaaaggtag tatctcaaat ggataaacaa
      901 tttcttcctg tatcaatata tagggtacag aaatctttat aaattgacta tgttcaccta
      961 taaaatcaaa aaatgcaatt agtatttccc ttaattgctg tttaggtgct aaatttttag
     1021 tatcatgtgt agccttatat tcatccatat gacgctttaa aaatttttta atagaaatat
     1081 taattaactc ctctttagat ttaaaataat aattaatcat agctaaattg acatttgctt
     1141 tagctacaat ttctctagcc gtaatgcttg aaacatctct atgtgaagat agtaactgga
     1201 ttgtgacatt aattaattgt tcctttgttg aatctgcttt cataattttt ccccttaaaa
     1261 gtttctccat aaaaaaatta aacatgttta atacacgttt agatactatc atgaaaattt
     1321 tgggatgtca atgggtgatt agtacttaag tatttaagta ctaatcgatt agacttttat
     1381 aagggggatt tgaatttctg taagataatt ttcaggattt atttcattat gttcaccttt
     1441 atgaccgatt tgtctattta tacctgatat ttgataatca ctatttttat ctaaccaata
     1501 acagaacgat tcatatgcac cagctatatt ttcatatgga ccataaacca tagtacatgc
     1561 catcatatca atttcttctg tctcacgata tataaatcca gatttgttct ttccaatctt
     1621 ctttacaacc attccaactt caatatctac accattatct ttgacttctt catcatggta
     1681 aaaagctatg ttattgttgg attgacgtga tacttctata tgttcttctt tgataaattt
     1741 agatagcttt tcccagagta taccttcaga ttggtagtct ggaattatct ctctaagtga
     1801 tatgatttta taacttggaa tctttttaaa gacaacattg taatgaattt catctttatc
     1861 acagtttata gcttctataa atttatcaat tttatttatt ctttgttgtt cctgctttat
     1921 ttccttttgt atttcattct tttttctatt caattcctta attacaaatt catcattcca
     1981 attatgtacg atatttgcta tttctgcaac gctaaatttt gaatctctca ataatacaat
     2041 tctttgtaat attgaaatct gttcaactga gtataatcta tgacctgtat atttgtctgt
     2101 ttttgcaggt tttaaaatac caagttcatc ataataacgc aacatacgaa tagaaatttg
     2161 tgttagtttt gaaacctctc ctattttaaa catgaaaata tgacctcctt ttcacttata
     2221 atcagtatat attttgtttt tatatacttc aagcgtttgt ggaaatacaa ttattttcca
     2281 gatgttgcaa taaattatag agagtaaatt atatttaagt agtaatcgta tcaaatcata
     2341 gattatctta atttataatt catattaaaa ttaccagtct aactcaaatt caaaatcatc
     2401 cgactttatc tgttcaattt cagaatttac cttctgttta aatttattag aagaaatcat
     2461 atatacaaga aatattaaac caaatattat ttgtacatat tgagctatta cacataattt
     2521 aatagggtag tatagaagta attgaatgtt gaaaaaatag tacatcatat gaactaatcc
     2581 agctgataaa atgcttctac ttgtattgat atatacaaaa ttaattataa agtttaaaag
     2641 tacaatgttt ggtataaata aaaaagcatc aaatagggag tattgtgcca aattatatgg
     2701 tatttgatta ggtgtaaaat atgaccctag ataccagatt ccacaaacaa gccctaaaat
     2761 aattgaagag cctataaatc caaatctaac taataatttg tcaatggcat atcctctcca
     2821 tccagattct tgactaagta tactcaatat aaaacttgct aatagcatat aagctatttt
     2881 agaaggtcta aacactgcta tgtgtatcca atctatatca gaagttttta cattaaagta
     2941 atttccgatt attaacgaag taataaaagt taaagcaaaa aataatattg ttaatagtat
     3001 ccattttatt ctaatttctt taaagcttag gtatctataa ataaaatctt gttttgcact
     3061 ttgtggatag attgtagaaa taaaaaatag acctacaata gctggtgatg tatatgcaag
     3121 ataaaaaatg atttctccaa gtattgtatt tgtaattccg agcaacgcag gtgtaagact
     3181 gaatatccag ttcaatacta gcgtaaatgt aaagaaatag attaagtgtt tgtttgtatt
     3241 aaagggggtg ttatatagtt gcattttttc actatccttc cgttttataa tgaagtttta
     3301 atttttatta attgtttatt tatttttata tgtattaagt tatcgcttga tgttagttat
     3361 tgcctataat aatatcctat tttttatatt ttgattaatt atttttacac taaatataat
     3421 taattagtat ttaatattga atatattata ttattattaa ttattaaata ggtcaataaa
     3481 tttgcaatta gtgatattaa taaattactt tagattttta ttaaatgttt agcttttctt
     3541 taatattttt tatataacga cgatttataa taactttttc gttgtttttt aaagtagctt
     3601 gcatgcgatt atgtgatatt acttttatac tttttatgta gtccatattt aaaatcatgg
     3661 atttagatat tcgtgagaag gatgtattag ctaataattt tgataattta tatattttta
     3721 aatcaatttc aaatacatca tcttcataat aagcaaatgt tttatttcca actgtctcaa
     3781 agtataaaat atcacagaca ttgagtttat aaatctctcc tgatttttta ccttttatat
     3841 caagtaacgg tgtactcaat aatgaaatta ttttttcaat gttattatct agagcagaac
     3901 attttatggt tatttcaata tcatcacaat taataccttg ttcgacatgt attttcatta
     3961 atcatcacct cattttatta ctgtcattag aatagcatta tatcttattg agtccaacta
     4021 gatgcgttct aaatttcaaa ttaagcatag tgaaattcaa aaataaaaca gtgaaattca
     4081 aatttgaggt ggtaaaattc aaaaaaatac tatggatatt tggataaatt atccaaatat
     4141 cctagtattt tacatagaat atgcctaatt attttacaaa tagacctatt ttatgactta
     4201 catatattgt caaattactt cttaaatatc aaaatatttt tggataatgt atcaatactg
     4261 tttgataaag tatttaattt accctcaatt ctaaccaaca agtacatgga tagagctata
     4321 ggaaaaccaa cattggttat taacagttgt aaactatcat ccataaaatt agccccctat
     4381 tctataacta agtcataagg agtggtattt gtttccacaa ccttggcttc aaccacagaa
     4441 gctaggtcaa atcctccaac cgaaaaaata tttttagata ctatcaaatc catgacagat
     4501 tttatatttt cttcagttaa atcatccttt ggattttcta tagaaaaaga aacttgttta
     4561 tcctttacat ttttaaagct catgactaat tttttcttaa tttccatata attacctccg
     4621 atataataat ttatatttta attgtttgct tgttaatatt ggtattttaa ttatcaatat
     4681 tagtgttttg cttgttagta ttaatatttc aattgtcaat attagtgttt tgcttgttag
     4741 tattaatatt tcaattgtca atattagtgt tttgtttgtt agtatcagtg tttcaattgt
     4801 caatattagt gttttgtttg ttagtattaa tatttcaatt gtcaatatta gtgttttgct
     4861 tgttagtatc agtgtttcaa ttgtcaatat tagtatttta attgttgact ctagttattg
     4921 ctgatataga aaatatttaa tttatttcaa ttaattagat taaagtaggg tagcatctaa
     4981 aatagctaac atgtttaatt ataaatcaca aagtaatttt tgactatctt ttcaaaagtt
     5041 cttatatgcc ggtatagcct attgttacag gcttttccgg catttttcgt catggaagat
     5101 attctcaaat cttcttatat ccccgtatgt tttggtgttc aaaagtagta tgtggagtag
     5161 taaattctcc atttactact atgcaatcag ccgcttcatt tctgttattg acgtttcgga
     5221 agttgtgtgt gcgtaatagt tcagcgtcat attgatgttg gaatgtccca tgatgtactg
     5281 caatgctttg gggttcattc ccgcatttgc taacgtggtg cagaatgtgt ggcgtaaggt
     5341 gtgcggtgtc attacctttg gcaatgctac cttttgcgtt ttgttgtatt tccttacaag
     5401 cccccggaac atactttcat agttgactgc cacttttggc aaaccgttcc ggttaaggaa
     5461 cagaaacttt gtgtagccct ctaaaataac aggctgtgta tatttccgtt tgttcagcac
     5521 ccgctgaaat gcttccaaca ctttttcact cataggaata atccggttgc cgcttttagt
     5581 cttaggcgtt tctacatagt agcccacgtc tgcaatcttc aaaagttggt ggtctacatt
     5641 gataagctgt ttatctaagt caatgtcagc ttccgtcaat ccgcagagtt ccgaaatgcg
     5701 aagccctgtc cctaacagta tgataatctc gtcgtagtat ttctgataga ctttatcatg
     5761 ctgcacaaag gacaggaaag ccgcttcctg cgtaggggat agaggttcct ttggttccgt
     5821 atcatcttca aggactgtgt tcaactggaa gtcaaacgga tttttacgaa tacaatcgtc
     5881 ctgtatggct gtgtaaaagg cagcctttaa ggaacgcttg tggttgttga tagtcttgaa
     5941 gccataacct ttttctttca tgcgtaatgc ccattccttt gcgtcggaga gctttacatt
     6001 ttcaatccgg caggctccaa gtttatcctc ctgcaaaatc cgcatgagct gttttcgccc
     6061 ctgctttgta ccatgcctca catttgcccg gtggcgtatc tgctttgcgt aaagctggca
     6121 gactgtcatt ttctttccga tatggtcgat accgtcgtca aggtcttttt ggatttcttt
     6181 ctctttttcc ctcaacgcta tatcgtcgcg ctttcctgcc ggggttttgt ccgtaggtac
     6241 taacttccaa gaatacacaa actgcggttt tccaaaggta tctatatatt tgtaggcata
     6301 tcttccgtct tttctctggc tctctccggt gcggagaata cgatttttat tatcccgtct
     6361 tttttccgac atcgttatgc tcctttccat aatggaaaga gccttgatat gcttgactac
     6421 attgtaccac attcaaggct cgatttcact attagattgc gtccaccgta tcaatcattt
     6481 tttcaaattg ctttcgctta atctgaatac ggtttccgtt catcattacc cagtccggga
     6541 cgggattttc ttctgccagt ttacgcagct tactttctcc gatacggaaa tattttgcag
     6601 cttcttctat ggtaagggtg tatttttccc aaatcggcac gtcgttattg ttcattttgt
     6661 cgtccccctt tctcaatggg tcaaaagtca atcagtaaca taggctttaa tcggacggct
     6721 gttagcgcaa cctcatggga cttgcacccc tccggcggtc tgccgaagcc ctacccattg
     6781 cctgcgacgc ttctaacgct cggactatgg ctgtaaggaa gtatcattat gtattttgtg
     6841 cgttatcgcc cgcagtctgc tatatctgct ttgcggcgtg gtggttctcg ctcgcttttc
     6901 cagtaggggg aaaggtcatg gcgcacccgc cgtatggctc ggcacaaaag gaacgtatcc
     6961 gatttgcctt atgctgcgtc gccgttttct tgcgccgctt tctttgtcaa agagcagggg
     7021 gctgtcttgc ggtatggaac ggaaaagggg aaagaagcgt tccaatatcg cataggttat
     7081 tcagcccgaa aagtgaggat agccctcatc agtcttgctt gcagtcgttc ccgaatatcc
     7141 tcgtcaacgc cataatagac attgccgcgc tcgtcgtaga gcttccgcat ggaaagactt
     7201 gctatgtagc cgctgaaatg ctgcaagacc attttcatag cgtccgggtc gcctttgctc
     7261 gccgccaaaa taacgggata gggaacaagg gcattttccg ggtagtcgtt ttgattacca
     7321 ttcgttccat tcatcagcgt gttcctccaa atatttcttt aacagttcaa aagagcttgt
     7381 ccgtctgtac tgtatcgtgc tgcgcgatgt attgaacatc tttccgattt ccacatcagt
     7441 catgccctcg aaatagtaca gaagtatggc agttctcttt tcttccggca gggtgcgtat
     7501 tgcttcaagc aataacttcg gggttatttc ttttcctgcc atttgataag tcggctcggc
     7561 tgtttcagct tgaaaatact tatccgtagt gtaaagctgc cgttcctcat gcaaggcaag
     7621 gtcacaaaaa gaaacttccc gtttcctgcg tcgccggatt tcatcatggg cgttgcaggc
     7681 ttcgttgtgc agcacccgtt tacagaaact ttgaaaggca cattgcttct gaaattctct
     7741 gcgattaggt tccatgtaat cacctccttt ctgccgaaag gcgggtagta cctccccttt
     7801 tcgcgggata atacagacga ctttttcaaa cgccggacga actggaaaat ttatttgaaa
     7861 aatcttctgt aaagtgcaaa atgcgcctgc ccgcaaaaag gcgcggacag gcgcaaagga
     7921 atagtaaaca gtatttagat gtattgatag ttcatattca tagccagact tacccggtgg
     7981 gggaactgta ctttttttaa tgaatgtaac ctatgtatgc tgtatagcaa caaagaaatc
     8041 cccatagcgg cagccctcct aaatgccgct aatttatgtt ataggttgtc gtgatttagc
     8101 agtagacaaa aaaagcggcg gctctaaaaa tcaaagccac cgcttcaaag gcgcgtgaaa
     8161 gtgtatctgc tctgcgacag ctttttttct tttgccgtcg ttcggcaggt actatcaatc
     8221 aaattattta ccaacccttt tttctaactg cttttttctt cttaccaaca gtcaatgtac
     8281 cgggttcttt ctttttattt gcaagaataa tcatagaaat caagaaagtc aaaactattg
     8341 taatgaaagc ggggaaaatg tgataagtat aaccatattc cgtaccacaa gtagggtcta
     8401 ctaaaatcat gcttgcttta atcggataat aatttaacag tgttccttca aaagttccaa
     8461 aatatccata cacaaaagct agtgcaactc cacccaaaaa attatttgcc gaacaacaga
     8521 atatcagaat aatcggcaag actgaaatat aaattcctat attcgcccca gtaacacgaa
     8581 taaacatatt gagtagattg ccaaaatgaa ctcccggaaa tccgacaata atattgatta
     8641 ctaatgctat cacacaacct ataagactaa agctgattgt caacagcaat agaataagta
     8701 attttccaga aagtaactgt ttatatggaa taggaattgt taatatattc ttcatagtat
     8761 cgtctgtata ttcccttgtt atgatataac ctgcaatcag cgcaataata atcgggaaaa
     8821 ataaggtaca gttactaatc ataacctgtt gcataaaata gtcaaacgtc catgtctgtc
     8881 caccgttggc tgtggaataa aacagtgaca gcaacggaga aagtgttgtc ataataattc
     8941 cggcttttat tacagaatat cgttttaatt ttaaaaattc agtttttatt atatttctca
     9001 tatcttttac ctcgtccatt ttttataaaa gcgaattatc agccacaaag aaagtataac
     9061 tgtaattgcc ataataaaaa ttgtatggca agtggttggt acaatcgcat aggcttccgg
     9121 caaaagatta tctttttgca aattatccat cattaagccg cttgtccagt tcataacaca
     9181 tataaccgga aaataattta agaaagcaat tttggcagca cttatagatg tcccaatcgt
     9241 tcccagtatg ccccagttaa aaatggaata aaaaaatgca agcaggatag aaagtaaaaa
     9301 acttttgtta aagtaaataa ttaaaaatac aatcggtaaa gaagctgcta cgataagaac
     9361 tccaaaaata aggctcataa atatcttata agtcataccg taaaccattc ctacatgaaa
     9421 caatttacaa aacaacgcaa cagataatgt gcttatcaga caaaatgcaa cgctgaaaat
     9481 aaagagcata gaaatttttg ccataatgag ttgtgtattt gtaactggaa ttgttctaag
     9541 gtttttggaa gtatcacaat ctctttctat aaaaaacaaa atagcggcaa tgataccaat
     9601 tagacatgga agcaaaaaaa ctaaaccata tcccaacatc attgtataaa cataatcaaa
     9661 tcgggtttga agatagtgtt ccgttgtatc tgcaccggtt cccgcctttg ccatatatgc
     9721 caaaatcaac ggaaaaaata acgacaatga catcagtgca tacaacagtc tttttcgctt
     9781 taatttccaa aattcgcact taatcagttt aagcaattcc ttcgcctcct gtcacacgct
     9841 tgaagtaatc ttcaagactt tcttcacaag tatgggcttc tgatacctcc aatccgtttt
     9901 ctacaaaagc agtcacgatt tttcctacgg acaaatcaag attatgcaag cgtaaattat
     9961 ggtcgtcctg tatagagaaa tggctctcat ggaaagtacg ctctaaaatt cttgctgcct
    10021 gtgcggtatc agaaagagta aatcggatat gtttgctgct tttttgttcc agttccgcaa
    10081 gactttcttc ttccaatagt gtgccatggt cgataattcc aatatcatct gctagcagag
    10141 aaatctccga aaggatatga ctggaaatta aaattgtttt tccctttgta tcgcaaagct
    10201 cgcgaataaa agaacgtact tctgcaattc caatcgggtc aagcccattg ataggttcat
    10261 ctaaaatcag aagttcaggg tcgtgcataa ctgcaagtgc aatcgcaagc cgttgtttca
    10321 taccaagaga atactgggaa aacagttttt tgtctttata aggcaatcct accaaatcca
    10381 atgtgttttt aattgaatga cggttgggta tgccccgtaa ggtagcaaaa atttgtaagt
    10441 tttccgtagc agtcaaatta gggtaaaaac cgggcgactc aatcagacta ccgatacggg
    10501 gcaacaattt cttttcattc ccctgcaaag actttcccca tattttaact tcgccggaag
    10561 ttggctttgt caatccaagt agcattttca tagtggtcgt ttttccggct ccgtttcttc
    10621 caagcagtcc gtaaattctc cctttctgca catgaatatt taagtcggcg acactctttt
    10681 gtgagccgta ctgctttgta agattttttg tttcaatgat ataatttttg tccatattca
    10741 aacctcctgt tctgtaaagt attctatcat accaaccttg cattaacctt gccgcaacct
    10801 tgcattaacc ttgcaatttg aaatattgtc ccaaaaataa ttaaggctcc cgtcaaaagg
    10861 acgggagaac cttaatttgc taagggaaaa agcaaagtaa attccgttcc tgttccctgt
    10921 atgctatttg ctgttattgt gccgttcatt ttttctacaa gttggtgtgc gatagaaagc
    10981 ccaagaccgc tgcctttttc agaccgtccc ttatcgcatt tatagagccg ttcaaaaata
    11041 tgcttcaaat cttctttttc aatccctatt ccattatctg ctaatcggat ttgcatattt
    11101 ttttcctgct ttgacaggat tatttctatc ttatccgcat gactgtgaga aattacattc
    11161 tgaataagat tgttcaatat ccgcatatag ccgtccgtat caagttttac ccggaaaggc
    11221 tgttcgggaa tatcaatatt gtaatctatc tgcttatctt caaatatggg tatccagtca
    11281 atcaagatat ttcgtgtcag ctccgcagct tcaacaatat ttatatccat agcaaactca
    11341 ttagaattta atttgaacca gtcaaaaaga acatcaatat attctttcaa atcgtgagct
    11401 ttgcggcggg ctgtttcgat atagtcgtcc ctgtcttttc ctgtaacaat tcctttatgt
    11461 gcagcgtcaa gatacccaat aagcgttgta agcggtgtcc gaacatcatg ggaaagactt
    11521 gtcataagct gtcggtttgt ttcttctgtc tgccggacag tagaaagcct gctctcataa
    11581 gacacaacaa tctcatttat ttcataggca agaggggcta ccagttcatt tgttgcagat
    11641 aaaatacgcc tgtttccatt tccgtttttt acatcaatca atgcgtctgt tatctcggct
    11701 atctgttttt taacacgtcg aatagcaaag caggaaacaa gaatagagaa aagtgcaatt
    11761 attatggata gaaaaatgac tgcttccata gaaaatcaaa cctccttgtt aaaacgataa
    11821 ccgatacctt taatcgtttg aatatatttt ggacttccgg gatttacctc taatttcttg
    11881 cgaagtcggc tgataatcgc cataatgttg ctgtcatcat agaaatattc ctctctccac
    11941 acttcttcat aaatctgttg ctttgtcaga attttccctt gatttttcgc gcagtataag
    12001 agtaagtcaa attcttttgg aggcaattca aaagttccat tttccgtagt aacagaacga
    12061 ttttcaatat caattttcaa cccgtcaaat tcaagttgct gtgatattcc gtcctgttga
    12121 ttgaaacggg tataacggcg tataagggaa atgatacggg ctatcagttc gtccatgtca
    12181 aaaggttttg tcaaataatc atcagctcct gcccttaaac cgcgtacttt agaagcactg
    12241 tcattttttg atgtaaacat caaaatcggc aggctgtttt cttttctaat ttcttccaat
    12301 gtttcaaatc cgtccatacc gggcatcata atgtccagta tcacaagttg atattctctc
    12361 tcttttaatt tttgtaatcc ctcttttccc gtattacaaa aatcagcttc tatatcttct
    12421 gatagtacgc tgcgtttaat cagcgcacaa agttccttat catcatctat aatcaaaact
    12481 ttattcatgg tatagctcct ttccttgttt ggttctaccg tcaatcggtt tttccgcgtc
    12541 ttttggtatc aaccacatat acccaagttt tgaaacaccc tgtatgcgtt tctccctgca
    12601 aagtatctgt acccgccttt cagaaattcc ccacttcttt gctgcttcgg ggcaagacat
    12661 atattccata acaacacccg tcctttctga aaatcttatc ataataatta tagtcggaca
    12721 cacgaacaat agcaagtata taaatatgaa ttattatggc tatttagtgg aactgtataa
    12781 acatatccaa ggagaaaggg gaacagtaaa atgtaactgg gtgaataaat atggcaaaaa
    12841 gaccggtaga aaaatatgac ttcaaggcat ttggagaagc tataaaagct gcgcggacgg
    12901 ggcgcaaaga aagccgcaaa caggtaagtg atgaaatgta tatttctcct cgctatcttg
    12961 caaatatcga aaacaaagga cagcacccca gcctgcaaat cttttttgag cttatgcttc
    13021 gttacaatat atccgtagac cagtttcttt tagatacgcc cgccccaaaa gatacaaagc
    13081 ggcggcagct cgacgctctc cttgataata tgagcgataa tggcatacgg attgtaacgg
    13141 caactgcaaa agaaatttca gaagtcgaaa aagaggacaa ataaatgtgt ccggcaaatt
    13201 gaatatgatt taggaagcgt tgtaatcttt acagattgca gcgtttttct tttgctcttt
    13261 tttggcatat agagcattgc gttgtagtaa ccagtgagga aaaaactttc gtagcaagca
    13321 taggaaaaga gtaccatttt ccgtattttc gccctcccgc cctgcggtgc gtcggttgaa
    13381 aattgcttgt tgggaagtgt cccaaaccct caaagcggaa aggagcgatt gcatgaacgg
    13441 acgcaaaaga aaaatacaaa tcaaattcta tgtaacagag gaagaaaggg cattgattga
    13501 gcagaaaatg aagctcgtcc ccacccggaa catggcggcg tatcttcgca agattgccat
    13561 tgacgggtat atcattcaga cagaccatag cgacataaaa gccatgacag cggagataca
    13621 gaaaatcggg gtcaatatca atcagattgc aaagcgtgtc aacgcgacag gcagcgtcta
    13681 ccaagaggac atagaggaaa taaagggggt gcttgcggag atatggcggt tacaaagatt
    13741 aagcctatta aaagcacgtt gagcaaagcc cttgactata tccaaaaccc ggataaaaca
    13801 gacgggaaaa tgcttgtgtc ctcttttggg tgcagctatg aaacggcaga tattgagttt
    13861 ggatttacgc ttgcacaagc catagagaaa gggaacaatc tcgcccacca tttgatacag
    13921 tcctttgagc cgggggaagt ggattatgag aaagcccacg aaatcgggaa acagcttgcc
    13981 gacgcggtaa caaagggaca gcatgaatac gtcttaacta cccacataga caaagggcat
    14041 atccataacc atatcatttt ttgtgcggtc aattttgttg actaccacaa atacaattcc
    14101 aataaacgca gctactatgg catacggaac atgagcgata agctgtgccg ggaaaacggg
    14161 ctgtccgttg tcgttccggg taagggcagc aagggaaaaa gctatgcaga atatcaagcg
    14221 gaaaaggtcg gtgcaagttg gaaaggcaag ctgaaaatta ccgttgatac gcttatcccc
    14281 caagtggcaa gttttgaaga attgttgcag cggttacagg cagcgggcta tgagataaag
    14341 ccgggaaaat atatctcctg ccgcgctccg ggacaggaac ggtttacccg cttaaaaacc
    14401 ctcggtgcag attatacaga ggaagcaatc aaagaacgga tagagggcaa gcgcacaaag
    14461 gcggcgaaag ctcccaaaga gcagcgcggc gtatcgctgc ttattgacat tgaaaacagt
    14521 atcaaggcag cgcagagccg gggctatgaa cagtgggcga aaatccataa tctgaaactg
    14581 gctgcaaaga ctatgaattt cttgacagaa cataagattg agcagtacgc agatttagtc
    14641 agccggattg aggaagtaaa tgcggaaaat gaaaagacag cagacgcatt aaagagcgtg
    14701 gagaaacgtc ttgcaaacat ggcggtgctg atgaagcacg ttaccaccta ccaaaagaca
    14761 aaacctgtct atgaagcata ccgcagggca aaggataagg acgcataccg ggcaaagcag
    14821 gaaagcagtc tgatactcca tgaagcagcg gcaaaggcac taaaaacggc gggcgtttcc
    14881 aagctcccga atgtcgccgc gctacaagta gactatgaaa agctccggga acagaaagaa
    14941 gccctttacg cggactatgg caaactgaaa aaacaggtca aagagtacga cgttatcaaa
    15001 cagaacatag acagcatttt acggcagcca agagagccgg aacgggaaaa agggaaagaa
    15061 cgcggggagt agctttgttc atacttgtgt gatataatag ttcctatcaa agacagtagc
    15121 cacatactaa agtgatacat tttcagcaaa ggaagtgtca gaaaatggaa caggaaatgc
    15181 cggactatga aacgatatgc gctgccgttg tgggcgaaaa atgggcgttg gaaaaggtgc
    15241 ttgattgtta cggcgacgaa atcaaccgcc ttgcaatggt aaaaaagtgt cagccggacg
    15301 ggactatcaa agaggaaatc gacgaagatt tacggcaaac actcataatg aaactgttag
    15361 aagctatccc acaatttcca atggaaaagg agtgatacca tgacacgcgg agaaatttgg
    15421 aaagactttt tcaagagaaa cgttttgccc gccgttattg cattgttttt attcttcatg
    15481 ttcaagaaca tctttacaga ggacgggtag accaattact tttatgtatg gctgtgctgc
    15541 ggtatcccct ttggcattcg ccgtatgttc gtatggcttg taccgcatgg atatgacata
    15601 gctggaacgg tgggtatcgt tgccattaac ttcattttgg acggcattat cggcgggggc
    15661 attttaatat ggcggcttgt cgttgctacg tggtatatcc cccttacgat ataccgttta
    15721 cttaccgcag ataagagtgc aacccctcaa atcaatatag agaaaggatt taaaagttat
    15781 gaatgaaaga gaaagaatta ttcatttatg gtttgatatg tggctaaaac aacaagattt
    15841 agggattgat agtattttta cagaagatgt gatttacact gaaagttgga gtcctaagta
    15901 tgaaagtcga aaaacagtaa aacattggtt tgatgaatgg aatactcgtg gaaaagtctt
    15961 agtgtgggat attaaacaat tttttcataa ggaaaatcaa actattgtag aatggtattt
    16021 caaaaacaaa atgaataatg gtaatattga agaatttgac ggaatttctt taatagtatg
    16081 gacggaaaat aacaaaataa tggagttgaa agaatttggt tgtaatctca atacctataa
    16141 tccatacgcc caaaatgata tgccacaatt cagagatgaa aaaacaaagt ggttttgaag
    16201 ttttaaaagc cgggacgcag acagtcggta aagactatcc gcgccccggc tttcttatgg
    16261 gtacagcgat aaaatgttac gcagtagaac gccatttttg agggaaaagg tataaaaccc
    16321 ttgccgcgtc attttcacac ccggaaagcc ctgtaaatca aggctttttt tgcccctact
    16381 gcgtaacaag ggcgcgtcgt ttcctcatgc gctccgctgc ctgtctgcgg gtaatacgct
    16441 tccggcaatc cgggcagtat ttgacgttgt tagaggtgga cgcaaaagaa gctccgcact
    16501 cggtacaacg ctttttatcc tcggtcttga aaagctctgc atatagcagc ttatcaaggg
    16561 gaagtacggc ggtctgaaac catttgcaaa gaagcgaata ggaaataagc tgcgggcaga
    16621 cacattcctc cccgtcgtcc agtaaaatac agtgtccgtt atcgcagttg cagcactctt
    16681 tttttacaag gctgttgact ttccggcttt ggggcggcgt aagccgcttt agcccggtca
    16741 tacctcatca gcggcgaaaa tggcaagctc cgcatactca ttttctgtca gaggtgcaat
    16801 tttcgcaagg gtgcgcttcg ccagttcccg catatcctcg tccataaacg gcagcgcagc
    16861 gttgatgttc tccataagcc cgcgcttacc gccctcattg taaatgctca acaggtttgt
    16921 ttcttcaacg gtcagtctaa tcatgctcat acctccaatt cgtgattttt tgattttgcc
    16981 gcttttttct gtgcggtttt ttctttgtct gctctaagct gcgcccggat agagggcttc
    17041 ttttccgttt ttgctgcctt gccgcgttcc ttgtcagcct tgacagcttc cgcaaggtca
    17101 acaagggaaa tggtttctcc tgcctttacc tttgcttcca gttctgcggt agtcggggtg
    17161 ttgtttatct gcccgtcaat catgttgtag ttttgctctg tggtctgctc ggcagctttc
    17221 agatagttct ccggctgaac gggaacagca aaagccctag tgccgttccc cataatcaga
    17281 tattttccgt cgtcggactg gtggtgcaat ccatatccgg cagcttccat ttgctcgcgg
    17341 ctcatggcgg ttacatagaa agtccctctc ggtgttttga ttgtttcgcc cgtttccaag
    17401 tcgtcgggaa tgagggcttt ttcctgctcc ttgaaaaact ccagcacttc cttataaccg
    17461 aagctgtcta catagtgggc ggtgtcctgc ccgttctgat gaagcaccac cacgtcggaa
    17521 acggaaaggc tgtgtccctt aaaatctttc gggtggtcga tattgaagcg ggtgtaaata
    17581 tcttcaaggg aagtccccgg cgtaagctct gcggaataga caagcgcata gtttttcggg
    17641 tctacccggt gtcctgtggc agtcagacgg tcgtaaggtt caaagcgcaa gtcccttgtt
    17701 tcttcctcgc cttttatctg ataaatggaa aaggtgtctt tgtcctgtgg ggcttcctgt
    17761 tccggctgct cctgtgcggg ttgttcttgt gcagctacct tttgcgcccg gatatgcgcc
    17821 cactgtaact cggtaggctt ttccccggta agaggtgcaa tcgccttgac ataatcagcc
    17881 gcatagtagg cattactggt aagctgccgc tgccatgccc ctgcttttgg cgaccaacgg
    17941 aagccattac tttttaaggc ttcccgcgtg gtttcgtcca gcttatcttc aaagaagatt
    18001 tgcagtcggt tcgcttcggt gtttgcttcc accttaccgc cgtcaaattc ccaacccaca
    18061 aaaccgattt ctttttgctg tgaaagggat ttgatacggt ctttcacgcg tcggatttct
    18121 gcgctgttgt tggataacgc ccaagtcgca aagggcttgt cctccaaatg ccaactgctc
    18181 gccatgtctg ctttcagttt ttccagttcc tccggcggta aatgcgggca gccgtcaagg
    18241 gtcttatgct tacggtaata ggcgtttacg gctttcatgg tttcctgtga tttttcaagg
    18301 ctttccagtt tcttttctaa cttctgtact gcctgtgggt cgtctgcgct gataccgccc
    18361 atgccggtgc tgcggatttt atcaagaagt ccttgtatgt cctgccattc ccggtaattg
    18421 ctgtcgcggg ctgcgttctg cttttctttc ttccttgtgg ggaagttaga gccgcccgca
    18481 atcaggatag agggaacacg cgcgtcaatc tcatagcctt tgttcatgtt cgccgccagt
    18541 ttccgggcgt aggtgtcaag caggctgtcg attttctcat ggtacatcgg gtcaacccgt
    18601 tgtttctgcc tttctgcgat ttgtacggct tcatcaacat aatgcctgta ttcagccgtc
    18661 gcgctgcctt gcttataatc ggaaaaactg ttcatatcct ttgcacgttt ggcggcagct
    18721 tcattgatag tgtaataagg ggcggcggtc tgcgtggtct gctccggctg tccgtcttgc
    18781 tcctttgcag gggctttttc ttcgggaagc tccggcgcgg ctatttctgt ttcgggctgt
    18841 ttggcggttt cctgtgcggg ctgcgcctgt tcggtggtct gttccttatt ctgtgtcttt
    18901 tggatttctg caaaatgtcc gtctatggta tcaatcagac ttgcggcggt actgcggatt
    18961 gtttcaagag agcctttcag ttctgcaagt tcttttccgc tgctccaccc ggcgatatat
    19021 ccaaaagaat aatccgacgt atcaagcccg taatgttggc agacagcata ggcgacgctt
    19081 tccgcctgta cctcgcgggt gcggcggtcg gggcgttccg gcatagcaag ggcggtatca
    19141 tgcagcgtgg cgtgggcgat ttcatggatt gcagtcttga tattctgcaa ttcgtccatg
    19201 ccctcgttga tgatgatacg cttttcctca taattgcagc gtccttttac gccgccgctt
    19261 agtgcttcaa atcccatagc aaaggggaaa gtcttttcaa gggcagcgaa aaaatccttg
    19321 tattgttcca cgttgcctgt cagctcatag gtaagggcag gaagctcctt tccctcggtc
    19381 tgtgaaatat caaagacaga taccacacga aaagcgggta tcttgatttc cctttcctcg
    19441 gtaacaggtt tcccgtcctt atcgaaaaca ggcttttggg tgtccgggtc gattttttcc
    19501 acctgtttct taacggtaaa cggggacggc gcaagtatct tgatagcttt ttctccgggc
    19561 tttacatggc ggtcaaattc cttttcccat tgtttatagc ctttcacaag attgccgccc
    19621 tgcatggcga taaggattgt attgttaagg ctgtaatcat ggaattttga catggtacgc
    19681 agataatcgg cgtaacggtc gctttcatac agtcctaaaa ttccttgttc cagtcggtcg
    19741 gtaatctctt tcattttgtc agcgggcttt tcagaagata ggataatcgg gagaaccggg
    19801 ggctgctctg tggcggtggt ttgtatggct acggaagtgt caaggtctgc ctgttccttt
    19861 tcctgtactg gcggctgcgg cgttacccgg tattcctccg gcacttcgca cccgtcgtac
    19921 cattctgtaa aggtgttgcg gttgttgtag atatagccct gttcggtaaa gctgcccccg
    19981 tcattgatag cagcgtcccg cccgtattcc tcatacatga aatatttttt tgcttcttcg
    20041 ggcaactcca actcgtccag ttcttcaatc agataatgcc cgtaatcttc ctcacactgt
    20101 atggacggat aaagccagta acaatcaagg ttttgtgcaa ggttgatgat gtcctgcaag
    20161 tcccttgtat ggtcgccgta ttccatagct gcaacaaatt tttcccggtc gtcgtcaaac
    20221 tgcattttca aaagttcgct caaatagttc agttcgtcgg ggtgggaaaa ttcgttaagg
    20281 tgtgttgtta aacccggaac ggaagattga aattctgtaa aataccattc tccatagttc
    20341 ttgaagtcta tcccgatacg gtcaaaggct tctttcaaat gctcggcagt cgtggggaat
    20401 gatacccatt cgcccgcagg tctgccctcg gtgtactttc caagattaga aagatacccg
    20461 cttaaaatcg tttcttccat gcgttcactc ctttcgtttt caatcatacg gtgcataagt
    20521 tcgtagtcct ccatgctcat gctcccgtca aaaatatagg ggtcgggttc ttcgccgtcc
    20581 cggtaggctt tttcagagaa aggcagcccc gcagcgtggg cggcggctct tgcttcctcg
    20641 ataagctccg gcggcagcct gttgtcgtgg gcttcgataa attccccgat attgctatat
    20701 ccgttttcgt agtggcggcg taccccggcg gtcagctcgt caaggctcat atcctcgcca
    20761 agatagccgg aataatagcc gtttaccaca acggcggcag ggtcagcctg cttgatttct
    20821 tccatgcggc ttctgtcctc cgggtagaga atatcgccca tttcaaggga aaaatgctcg
    20881 gcgttccaag aacgtcccgt tttccaaaac gccacccaag cgataccgtc ccttaattct
    20941 tcttgatagt cctttacggt gtcccgtaaa cttgccatgt gtaacctcct ttcttcactg
    21001 tcttagaggg tttgggaaac ttcccaacaa gcaaaaatcc ccgccgttct cggtagagaa
    21061 agcagggatt ttacggaaaa gggtactctt ttcctatgct tgctattcag ttatttcttc
    21121 ttcgacagtt caatgcggta gttgacatat ttctcgcctg tatcagccaa tgcgatagtt
    21181 ccgtcgtaag ttttgcctgt ctttggggaa tagagcttct tcacatttac cttgccggat
    21241 tttaagagtg cagcggcaat cttcggggta aaggttactt ttcgttcttc aaagaaacgg
    21301 tcatttttcc acatggtaaa gctacattct ttgttgctgc aatagtagtt tttctttccc
    21361 tcatagacag gagaaccaca gcggggacat ttcccaagct ccggcttttc ctctttgaaa
    21421 cgccccttgt cagcttcgga aagaaacgga taggtcttta ccagttctcg cgccatatcc
    21481 tcgataccct tcataaatgc ggcggggtct gccttgcctt tggcaatctg cgtcagattg
    21541 ttttcccatt ctgcggtaag ctgcgggctt gtcaaggtgt ccggcaggac gcacacaagg
    21601 ttgataccgt cttttgtggg aagtagctgc ttgccttttc gctccacaaa gccgcccttt
    21661 accagttttt caatgacggc ggcgcgggtg gcgggagttc caagcccttt gcgttctgcg
    21721 tccgggtcgg tgtcctcgct tccggcgcgc tccatagcgg aaaggagcga cgcttcattg
    21781 tggggctttg gcggtgttgt gtcatgctcc gttacctttg ccgctggatt gtcaaagttc
    21841 tgtccctcgg taaacggcgg cagggtcttt tctgtgtcgc tgtctgcgtc gtccgtttca
    21901 ggcttactct ttaagattgc cctgtatttt cggtcaatct ctttccaccc gtcggacagt
    21961 accgtttttc cccttgcggt aaaggactgt ccggcacact caaaaactgc cgtaaccgct
    22021 tcaaaggtat gcggtgcggc agtcgccata atcagacgcg ccccggcaag ggtaaggata
    22081 ttcctttcgc tttccggcag cgcggcaagg tcggtctttg caagctccat agtcgggata
    22141 atggcatggt ggtctgatac ctttttactg ttcagcacct ttgcaagctc cggcgtgaag
    22201 tcttttcccg ccataaaagg aaatttcccg caaagcagct tgataatgcc cgctgctgtg
    22261 tcgcccatgt cgtcagtcag atagctgctg tctgttctcg gataagtaag aagtcttttt
    22321 tcatacaggg cttgtgcaag gtcaagggtc tgctttgccg tatatccgaa caggcggttt
    22381 gcttccctct gtaaagaagt aaggtcaaag agcttcggcg gggctgcggt ctttttctct
    22441 ttcaaaaggg aagtacagac tgcttttgac gcttcgcaag tcgtttttaa tgtgtcggct
    22501 tctgccttat ttgaaattct ttcacttgca gcttccgcgc cggataaggc aaggcgcaca
    22561 tggtagtatt tttctttctt gaaagtggta atcgctgcgt cccggtcaac aagcattttt
    22621 aaggtcgggg tctgtacacg ccccacgttc aaggtatgat tgtaaaggac agagaaaagg
    22681 cgggtggcat tgataccgat tagccagtca gcctttgccc tgcatagtgc ggaagcaaag
    22741 agcgcgtcgt attccttgcc gttcttcaga cgggaaaagc cctctttgat tgcgctgtcc
    22801 tccatagaag aaatccaaag gcggcgcata ggtttcttgc agcccgccat ttcatagaca
    22861 aagcggaaaa tgagttctcc ctcgcgccct gcgtcgcagg cattgacgac ttcggaaaca
    22921 tctgcccggt gcataagctc ttttagggtt ttaaattgct tttccttgtc ggcagcaacg
    22981 gtgtatttcc attcctgcgg caaaatcggt aagctgtcat agttccactt tttatactgt
    23041 tccccgtagg cggcagcttc cgcaagccct accaaatgtc ccacgcacca agaaacgata
    23101 tagccgcttc ctgtgagaaa tccgtctttc ttttccttta ccccaagtac ggcggcgatt
    23161 gtctgcgcca cactgggttt ttctgcgata actaaaatca aatataagtt cctcctttca
    23221 gcgttccggc tcggtttctt ttttcttctg tacctgtttc agacagtacc ccacacaagg
    23281 ggactgtccc ttgtaagggc aggaagcgca agccggggga taaggaacag gcggcgcatt
    23341 atcacgccgc ccgttcggtt tctgtatcat catcttttca aagggattgt cggtaaacag
    23401 ggtcataggg tttcttcctc cgtttcttca tcttcgggag tttccccggt atctgcttcc
    23461 ggctcgtcct cgtcgtagtc ctcaaattca aaatcttcaa ggtcggtatc gccctttaca
    23521 ttctgcttcg gcttcataaa cttaaagtag tagaacgctg cgccgccacc aagcagggca
    23581 aagaggacta aggcaagcac cacaccggga ttgccgcctt tttctttcgg ttcttccggc
    23641 tgctcggttg gtgttggggt aggttctttg cctgtgcagc ttgtcatact ggtaacacat
    23701 accaggcagg acgtgtttac ctttccggct tcgcatttct ccgtacagtt gcagatttcc
    23761 ggctttttgg cagcttcgcc gttttcggtc agtgccataa ggtcggcttc gtccacttga
    23821 tttaggaaat gtacggtctg ttcgccctcg gcggcgcggt caatgaggat atagaaatag
    23881 ttgccgcttt tggtggtaac agtgataagc tgcttattgc cgccgtaatc gtcaaccagt
    23941 gttgcgtttc cctttggggt aaggggcggc gtttcttctt tttcctccac aactacgttg
    24001 ctgtcgttgg tcgcgtcccc ggcgggctgc tctgtggctg cgccctgtgc ataggcaggg
    24061 acagaaaaac cgcctataag gataagggcg gcacaaaggg cggtcatggt ctttttcagt
    24121 ttattcttca tctgcatttt cctcctgttc ttcgtagtct gccgtttcgg tagcgggtgc
    24181 ggcgttcatg ccgggaatac cgccgccgga cagcatagcg ttaagttcct gcggggtcag
    24241 tcgcatagct cgtacaagct gtacgatttc aaggttttcc gcttcggttt tctgcgcttc
    24301 cagtcctttt aacttgttct gatactctgt gattttctcg cgggtctttg cgatttcctt
    24361 attgatacgt tcgattttat tgtttgccat aggtcatttc tcctttcttt ttggggctgc
    24421 tttaccagtt catcagcccg tagcctttga tacattgata gttaaggtca tagcttttta
    24481 tcttgcaggc gtcgccggaa ttgccctcaa cggtatagac gcggctgccg tccctgccaa
    24541 gcacaagccc cacatggtcg gcaacgccgt ctaaatccca atcgaaaaag attgcgtcgc
    24601 ccggtgcgat attctcatag ccgcgtgcgc cccattgtcc ccttgactga aaccaaggga
    24661 caccctgcca ttcgcagccc gcaaagcggg gttcgctttt tccggcttga ttgtagcacc
    24721 atgatacaaa acaggcgcac cattccacgc ggctgttaaa gccgtaccaa gaccagtaag
    24781 ggtagccgcc cacatttccg acttgctgtt ttgcaagctc cataagctct tgatttccgg
    24841 ggcgcgtacc gtttacaaac tccacgccgc ttaaatcctc ggacgggctt ccgtcgggag
    24901 aaccgccgcc aaaaatcagc ggtttgttgc cgcttgtttc caagtacaca tggaacattt
    24961 caagctgctg tggcgttaaa agctccgaca caaaagagga aatcggcttg ttttccagtt
    25021 tgacattcaa gatgtaatac tcataaggga cttcctcggt ggtggtttcc ccggtttccg
    25081 ggtcggtgct tgtttcagtg cggtagcgta tttccacttc ctcggtcagc gtcaaggtgt
    25141 actgtttttc aaagatacgt tcaagctctg cctgtgcgct ctgcggggtg tagctctgca
    25201 aaagggcggt caagtaagac gcaagttcat gtgggttatg cccgatactg tcaaggtcat
    25261 agcggtattc atcatagccg gggtgggtgc tttcgatagc gtcaatctca ctttgcagcc
    25321 cgttttccaa tgcggcataa ttgttttcaa ctgccacaag gtcgctgtcc tcggacgtgt
    25381 aggaagtgcc aagcaccccg ttagtcaagc ccgaaaacat agccccgcaa gaggacagcc
    25441 ctacggaaat cataataaag agaagcagcg cggcaactgc aatcaaaaca cccgccgggt
    25501 gtcttgccgc aaatgcggcg gctttcctcg tttcttctgc ggttttcttc gctgtctttc
    25561 ttgtggcttc cgctgcggct tttgcccctc ctttctttgc cgcctttgca tactggcatt
    25621 ttatctgctg tttctgcatg aaacgggaaa gagggttaga agtaagctgc gggttgtcgt
    25681 gcagggtctt gtgatactgg aagttgacgt ttgccttaaa tgctgccttt tctgcctttg
    25741 ctgcttcccg gtaaggtttc agcttatggc tgcggtagcc ctgttttaac ttccgcgccc
    25801 cgtatttcgc gccgcgctcg gcaagttcct ccgatttatg cgccccctcc acgccggaat
    25861 tgtccttttc aacagaatgt atcttgttgt ggacgaaaat tcctgcttcc tgtgcggggc
    25921 gggacagcgg gtttcccttt ttgccgccgg gtatcggttt ttcctgttct tcaaagtgaa
    25981 gtcgggtttt tgcctttcct gtggcttcct caaaggtacg ctccttaacc agtttctttt
    26041 gcttcgggat agctgccttt gccgcgtcca gtttatcggc tgctttttct gattttttga
    26101 tgtagggttc cagttccggc gttgcccgtt cctcgtcggt aaactgcaag cgggaagtgg
    26161 aagtacgcaa agcagtagct tcctgctctt ttttgactgc ctttttgttt gcctttcggg
    26221 tatgtgctgc gtcaagatgt tctatgacgc gttccgctgc ggcactgtcc ggctgtgaaa
    26281 agtcgccgtc tgcttcccgg ctgctgatac gctcggcggt ctgctgtgtt tcgttgactt
    26341 ccaccacgcc gtcgcgggtc attttctgtg tgattttatc gcgtggtttt aattgcttga
    26401 tagtcctgca cctccaatcc gcaccctcgc aagggcgcaa tactctgcat ttaattcaat
    26461 cccgatataa tggcggtcaa ggctttttgc tgcaaatcct gtggttccgc ttccgaaaaa
    26521 cgggtctatg acaatcccac ctttgggaca gcctgccaag atacaggttt ccgctaattt
    26581 cgggggaaaa gctgcaaagt gcgccccttt atatgggacg gtgttaatca gccacacgtc
    26641 ccgcttgttc cgcgtagtcg gcatgagcgc gtcgtcatag tagccgccgt cccgcggtcg
    26701 gtttagtccc tgtacctttc cctgtccgga gatttcctgt gtgtatttac tgttagtgct
    26761 gcgcgctctg cgataccgtt ctgcggtggt gggggctatg ggttctgcaa tagcggcagc
    26821 gtcatagtaa tatttctttt cctttgaaag caggaagata tgttcatagc agcgggtcgg
    26881 gcggtctttg cagctttccg gcataggatt ttgtttctgc catataatat cgctccgcag
    26941 ataccacccc tgttcccgca aggaaaacgc taaaagccag ggaataccga ttaagtcctt
    27001 ttgcttgcag ccggtaacgc ggctgttcct tgcaacccgc tgtccggtgc gcccttttgg
    27061 gtttttcggg tcagcatagc cgcctttatt tcctgtgctg caataggtgt ctgcgatatt
    27121 cagccataac gtaccgtcct ttctaagcac ccggtaaagc tcgccaaaaa cagcggtcag
    27181 cctgtcaata tactgtttcg gcgtgtcctc ctgtccgatt tgtgcgtcaa gcccatagtc
    27241 ccgtagcgca tagtacggtg ggcttgtaac gcagcaatgg acgctttcag agggcagttc
    27301 ttttaaggcg gcaagtgcgt ccctgttgat aatcacatct gttttcatag gctgctcact
    27361 tcctccggct tcgtcgtcat tacccggtaa agctcggtat ctttcgggaa gcggtctaca
    27421 aaaggcagca ccacatttcc ataaaagata agcccctcgc ccgcttcggt gtggataaca
    27481 tacttcatct gctgcgggga aatattaagc tgctttgcaa ggatagtacg gtcgccctgc
    27541 gcttgattga gcataaggac aaaatcgctg ttctcaaaga tattttccac ctcgcggctt
    27601 gccaaaaggt ctttgacgtt ctgcgtaatg gctgtgggta tgccgcccca tttacggaaa
    27661 cgcttccaaa tctccacgct gtaagctgcc gtctgttcct cttttaagag caagtgaaac
    27721 tcgtccatat agtagcgggt ggatttcttt tctgcccggt ttatcgttac cctgttccat
    27781 gtctggtctt gcacaatgag catacctaac tttttaagct gcttcccaag ctgctttata
    27841 tcaaagcaga caaggcggtt agaaagttcc acgttggttc tgtggttgaa tacgttaagg
    27901 ctgccggaaa catacagctc caatgccgcc gcgatacggg cagcttccgg ctccggctgc
    27961 cttaacagct cgtcgtaaag gtcgcccaag ataggcattt ttgccgggtc ggggtctgca
    28021 aggaacgggc ggtacacatt tcttacagcc ctgtcaatga cggtcttatc gacaggctgc
    28081 aagccctcct tgccgccaat aacaagctcg cagagggaaa ggataaaatc ggactttaag
    28141 gctaacaggt tgtcgtcctc gctgtaatta aggtttatat ccatagggtt cacatactgg
    28201 ggcttgccgt caatgcccct gcccgtaggg gaaagacgta tcacttgccc attaagacgc
    28261 tgcacaaggg aaaaatactc ggcttccggg tcgcagatga taatgtcgtc gtcggtaatg
    28321 agaaaagcgt ttgtcatttc cctttttgcc gcaaaggatt ttccgcttcc cggcgttccc
    28381 aagataagcc cgtttgggtt tttgagctgc tttctgtcgc agagtatcat gttgttagaa
    28441 agggcgttca agccatagta aagggcttgc cccgtctgaa aaagctcctg cgtgataaag
    28501 ggaatgaaaa tagcggtgct gctcgttgtc aaacctctct gaatagggat aaggttttcc
    28561 ccaatcggca cactggataa aagccccgct tcctgctgat agtcaaggcg tgtcaggcgg
    28621 cagttgtatt tctgtgcgat acccgccgcc gcgaaaatgt cattttccag tttccgcttc
    28681 gtgtctgcca tgtttaccac aaggaacgta aggagaaaca ttcgctcgtt gcggctctgt
    28741 aagtcctgca acaggttctt tgcttcgctg ccaaaggtgg caaggtcgga cgggattata
    28801 tccatgtcat agccgcttct aaccgctttt ttctgttcct caatcttcat cttgtcaagg
    28861 tctgtgattt tgcgcttaat ggtcttgatt gcttccgact ggtcgatact gcggatatgt
    28921 aagttgacga ttacgcctgt ttccaagtcc agtatgtcgg caagcatacg gtcgttgagt
    28981 tccggcgcaa ggatttcaag gaagctcgcc gcgccgattt tcttccccat gcggaaatat
    29041 ctgccctcgc caaaatggaa agaggacggg gcgataaagt cctttgtagt aagccccgac
    29101 ggggtaagcc agtcaaagga aaaggagaac ggctcgccct ccgggtggaa taccccatgc
    29161 agcaccttta agtgttcata gccggataag gggcgggcgg ttacgccaag caccttgaaa
    29221 ttgttaagta cgtcggtttc gatacgggaa agccttgact ttgcagccgc cttgttatca
    29281 gcttcaacgg taaaggtaat gtatttgtgc ttcacaagcc cgttgttgcc tttactaagc
    29341 tgatttttca gcatatcgga atactcggtg cggatagagt tgaaagcgtc ctcctgtgcc
    29401 gggatagaga tagctttttc tgcctgttcc cgctgcgtcc cttgattgat gaaagaaagc
    29461 tgcacgctca cgctcgcgtc aaagtaattg aggaaatcgc accagttttc aaaaatggcg
    29521 gtcttatcgt cagcctgtgc aagctgataa ttgatgtcct cgaacatgac gcactttgaa
    29581 tatctctttt ccgtaacctt gcagataccg tccgggtaca tggcaaggta gggaatggtc
    29641 tgctgtgcgg tgcgggcttt gccgtcgcct tttgcttttc ggatagcttc ggcaatctgt
    29701 ttcttttcgg cgcgggtcag cttacgcctg tttcctgcgg cttcccgcgt cttttttgtt
    29761 tgtccttttg acaatcgctg atacctcctt ttcaagtttt cgctgctttt ccaagacagc
    29821 gtataggttt tcggttctgt aaggtcgctc ctttgggcgg gtaaactttg tctgaatgat
    29881 gtttcttacc accacttcaa ggggctgtcc atgcttttca tacatggcaa gcaggaagca
    29941 ggggagcatg acgacaatca tcgccatagc cgcaaggttt gtccctgcgc tgcctttgag
    30001 caaaaaaaac agcggcagtc cgattaagag agccgccgca aaacaaacga tttgccgctt
    30061 tgtcagattg aaagcgactt ttgttttgac tttggaaagg tctttgggta caggtacata
    30121 cgccaagaaa aaacctcctt tctgccctta gtgggcgttg aatatggatt ttgccagtgt
    30181 gccggatttg aacagggaaa aacagaggat aaccgtatag gcggcaaggg aaaataccgc
    30241 gctgtgcaga ttgtctgcga ttatcatatc gtttaccagt acagcgtaaa taccgacaca
    30301 tatcataatg aggaagcctt gaaaaccaag agcgaacagc ccttttaggt agttgttccc
    30361 aatctgtccc cattctctgt tagtcatggt tgcaaatgga ataggcgata ccgaacagta
    30421 aaggtaaatc tctatcattc tgccgtagag gataacggtt atcagtacgg acatgatttt
    30481 catgcaaagg ctcacaaggc ttgtttcaag cacaagtaag agcagttcgg ggatttccat
    30541 atcttcaagc ccctcctgca tggcggcaag ggcttcggca acgtcgatat tcgtatcgcc
    30601 gccgataacc cccgctgcgc cggaaacgat atgctgcgcc atatcaaaga ctgccattgt
    30661 gatgttgaac gtgttggtta cgataaacac cgcgacaaag gctttgaata tccacttaaa
    30721 gaacatgaat gtttctatgt cgtgcatatt gttcttttcc gttaccatgc tgattagctc
    30781 gtagcatagg acataggtaa tgacaagccc cgcaatcggg acgattacat tttcagaaag
    30841 ggtctgtatc atgctgaaaa tgtttgcgtt ccaaccctgc ggggtctgtc ctacctcgtt
    30901 tgcgatagtc ccgacttttt cgtttacgtc cccgaacata gttgacagat taccgtttat
    30961 cgctccgata aggatttcct ttatccattc gttaatcgcg tcaagtatgc tctgcatagg
    31021 cagctacccg cgcttaaccg aacaagccgg aaagcagagg tacaaggata gtgccgataa
    31081 gggcaacgcc gccgcccgcc atgagctgtt tcatacctaa atagtagaaa cggcaaacgg
    31141 cgggcggcgg cgtgatgttt ccggctgctt tccgggccta ttcggttgta tcattcgcgg
    31201 tcaagtctgc gcttgtccgc ttgcttcttg aaattgaaat ggatttccac ggtctggcga
    31261 acggtgctgc cgtccttgtc ctcgtggata acgatttttt ggataagccg gttgagcgtc
    31321 accgcgtcca attcctgcac gtccgcatag tccttgatga tttccagcca gcgggtgttg
    31381 tcctcctgct cccgtgcctg ctcgtcaagg cgctgccggt tcagcgtgac gcggttttta
    31441 agtacggcct gttcgccttg gctgcgttcc agaatccggt tgaaattgtc ctcggttatc
    31501 cggccctccg ccatatcctc atatacccgg cctatgacac gttccagcgt ggcgatacgt
    31561 tcctcgtcct ccgccacgct ttgcaggatt gcggcgcgct cggattcttc ctcggcccgg
    31621 cagctttctt tgagctggtc ggctgcgtcc tgctcgtccg caagggcggc tctggcacat
    31681 tcccgtatct gttccagaac gatactgtaa agcgcgtcat agctgatacg gtgctgggaa
    31741 caatgggcca cgccgtactt gttgtacttg gagcaggtga agattttttc cttgcttttg
    31801 gcgttggcgg tgcggatatt aagggcgctg ccgcattgtc cgcatttgac aagcccggca
    31861 aacaagctga aattgcccca tgcgtccggc cttttcctgt ccttgatttt ctgctgcaca
    31921 atgtcatagg tttcccggtc aatcagcggc tcgtgcatac cctcgacggc aatccagtcc
    31981 tcccgctttt tatcggacag ccagcccacc ttgaagcggt aaaccacctt ttgagaagct
    32041 atcacgccaa tatagacggg gttggaaaga atctccttga tggtggtaaa gtcccagata
    32101 aaccgcccat gctccgggtc ggcaatttca aacttactgc gcttgtcccg caagcccttt
    32161 ttccgattcc accagacggg ggcggggatt tcctcgtcct ccagcctgcg ccggatatag
    32221 ttggggccgt gtccgttgcg ggcgtagtcg tagatttttc gcacaatcca cgcggtttcc
    32281 tcgtcgggca gaagatggtt tttgtcctcc gggtctttcc ggtagccgaa cggggccaga
    32341 cagccggtaa acttgccgga tttcgctttc gtcaagtagg acgaatgtac tttcttgctc
    32401 acgtcccggc tatacatttc gttcaggata ttgcggaacg gggttatgtc gttctcaatg
    32461 tctgtgtcta ccgcgtcatt gagggcgata taccgtacat ggttgcgcgg gaaaaattcc
    32521 tccaatagct ggccggtctg caaatagttc cggcccaaac gggagaggtc tttcgtcaag
    32581 ataacgtcaa acctgccgcc ctccacgcct gcaagcaggc gtttcaggcc cggacgctcc
    32641 atgttcagcc cggtatatcc gtcgtcctca aacgtcgcgg ccacacgcca gccctgttcc
    32701 tcgcagtaac ggtgtaaaag ttctttctgg ttttgaatac ttgcgcttgt tccgtcttgg
    32761 tcgtcgtcct tggaaagacg gcagtaaacg gcggctctca cctccaaagg gtagtgttca
    32821 gcatacacgg tgttagggtt catttatgtc ctccattccg ctgtgtatgc cgcgtttagg
    32881 agtatcttca ttatactcca tcggtttaga attgtcaatt atagattgcg atttaacaat
    32941 ctcctgtttt tgcagaatca ggtcaataaa cgcctgccgg tcgctctgct ttccggcaaa
    33001 aacggcgctc actgtgataa cggggtcttt cggttttgcc ataggcggct cctttcagtc
    33061 gttgagaagc cgggaggctt cgtccttgtt ggcggcggcg ttgcccttgc ggtaattcgg
    33121 cccgttgaag catatcgggg tgcagcgttc cagaacgcgg tcatagattc gggcgtgtgc
    33181 taaatccttg ggggcttgca gctcggcaag cggtagattt gtcgtgatga tgaacggcag
    33241 gcggcttaaa tatcggctgt caacgacgtt gtaaacctgt tccagcgcgt attctgaatt
    33301 gcgctctatc cctaaatcgt cgatgattaa caggctgaaa tggtgaaagc tggcaagata
    33361 cttgtttctg ttctcgctga acattcctga taggctgttg agtatctttg agaagtttgt
    33421 cataagcacg gggacggcat gctcggtgag tacgtgggcg atacaggcgg cggcaaaggt
    33481 ctttccagtg ccaacgcctc cccaaagcaa aagcccctgt ccgcgttctt tcatggcggc
    33541 ccaatgttcc acatagcgcc gggcgtactt catagccgga ttctggccgt cgtccccggc
    33601 gaaggtatag gccaatagcg cccggtcttg tagggccgtt gctttcagcc tgcggattcg
    33661 gtcaaggcgt tcctgctccc ggcgggctgc ctccgctgcc tcccggcgct cctgttcgca
    33721 ttggcagagg cagggcatta gccgctccct ctcgaaaagc gtcacgcggc attgtttggc
    33781 cgtgtggcac ttgccgcaaa ataacaggcc ggttgccggg tcggtgtagt cctccggcgc
    33841 tggcgttgct gccgccgcga taccgtccat cattctttcc agctcgtccc tcataagctc
    33901 tcgccctcct tgtaggtgta gtcgggcagg ccgccgccct tggttccgct ggcggccttt
    33961 tggctgtcct cgctggccca gcgccggatt gtggcggcat ggctcttgta cttcctgccg
    34021 gtggattcca tgtaagcgga cagcttttcg acgtacttct cccagacggt gggaaactcc
    34081 gcttgtaggt cggcaagctc ggcctctgtc aaaagcacat tctggtatct gccataggtg
    34141 cgggcgggct gtcccttttt taactctctc tctatctctt tctctatctc taactctatc
    34201 tctatctctg gtggacgaac gtcggacaaa tgtccgccat ttgtccgcga cggtaaaagc
    34261 gcccggtttt tctgcctcgc ggcccgcttg cgctccccct cggtggagga ctggccgatt
    34321 agcagctcaa tgttgctcat gtagtacgcg ccgccgtcca gcagctccac aaggcccagc
    34381 ttcaagaaga tttgcagggc gcgttccacg gttccgattt gctggcgggt gatggtggct
    34441 atcatctgcg ccgtataggg gatattctcg tcaagctgaa gcctgccgcc gtgcttcagc
    34501 gatttcagat acagcttcag cagaatgttg gagtagagaa cgccgtcctg catactttcc
    34561 agcaggacga tggaatcctc gtcaaaatag ctctctttca gtttcaggta gtagtatttc
    34621 cgattgtcgg acatggggtg tccctccttt gggttctgat ggggtctgta tgcctttaga
    34681 agccgttttc cgggggcaaa ggtaaaatgt atcacgcccc ctttgacaag cgcccggaaa
    34741 gccgctgttt acgggccttt ttcaatgcct aaagcgtgac atttcaaccg ttgtttccgc
    34801 ttgcgctggg cggcccctat ccgcttcatg cgtccggcgc actccgggca atacttggcc
    34861 cggttggaag cgggccggaa catggccccg cagacggcgc agcgtttggc ctcggcacgg
    34921 taaagcaggg ccgtttcaag ctctctatcc agcggcagga cggcggcccg aaaccaacga
    34981 cacaacaggg agtaggaaat gctttggacg cagatacatt cctcgccgtc gtccagcgca
    35041 aggcagttcc cgccgtcata gttgcagcaa ttatgcacga gccgccgcgc ccggcgatac
    35101 tggcggtagt ccatgatggg gattgcttca cgctgcctgt tcatcggcgg gccgctcctg
    35161 ttcctccggc cgcatagccg ccgctgctgg cgcgggcggg gttggcggca agggcagccg
    35221 gatagtcatg gtgatggtga tattcaaaat cgtttctttc atcggtcaag ttcctttcgt
    35281 ctggattgtt cggttttagc aagctgttcg gcggccttgc gtagctgctc cacggccttg
    35341 acttcctcgc gcatggcccg catttcctga tatagcacgt cctttttcgc ggtgagagtg
    35401 tcggcctcgg cccgccactg tttgggcgtg acggcctcgc cggtggcctt taggccgtct
    35461 aggaaacggg cggcgctctc gtatagggta agctccgcgc cgtggcgctc cgcaaacttt
    35521 tcacgcttgc cgggcttcat gccgtcaagc tggcggcggg ttcccttgtg ctgctcgtac
    35581 tgcacccaca tttccagccg ttccgtaagg gtggctagtc ggcgctcggt ggtgcgtatg
    35641 tcgccccgta agtcatagta agcgccattc atggccgaca ctttttcata gagctgttgc
    35701 atggagataa tgccgttctg ttgtaggaaa ttgaataggg cggcgctttc tttgagggct
    35761 ttcactttgc cgtaccgggt cgccggttgg ctcatttcct gccgggcctg ccatagctgc
    35821 gccatgacgc tttccttgcc ctgcggcccc tcggcctttt ctttcgtcca gttgtagagg
    35881 cgcgttatac gggctttaat ctctttcaag agtttgttgt cggcggcgat ctgccggttg
    35941 acgctgccct tgtcggtggc aatcccgcgc cgctccatac gggtagcggc tactcccatg
    36001 tgaacggtgg gtaactgctc cacgccctgc cgcttgtagc tgcggtggtc gattcgttcc
    36061 ggcctgcccg tctgctctaa agctcggttg gcgtactcgg cccatgccgc ccgccactgt
    36121 tcggccttgt ccttttcgtt ccagtcggtg gtgtcggcgc ggtggttctt ccagccgcct
    36181 ttgccgtccg ggattcgctg gccccgtggg tctaggtcgt attccttgcg gcacttcgcg
    36241 ccccattcgc tggattgctc taacggtcgc atggtgagca tgatgtgggc gtgtgggttg
    36301 ccgtcaccct tgtcgtggag cgcaaagtcg gcgcacattc cagcggcgac aaagttatcc
    36361 cgaacgtagg cccgaacaag ggcaagctgg gcctcccggt caagctcggc gggcagggcg
    36421 atttcaattt cccgtgcaag ctgcgccttg ccggattttt cgatttcctc cacgctgttc
    36481 cagagggtgg aacggtcatg aaacgccggg ggtgcgtggg tcggtagcag gatttccgaa
    36541 tggataacac cgcccttgcg ggtatagtcg tgggtctggc cgtcccactc gttggttagc
    36601 ttctggccgc ttcggtaggc ggctgcggcg acggctgaac ggccctcgct gcgcttgata
    36661 atcttgatgg tacagtgata gatggctatg gtcgcccgcc tcctttcggg tgggggataa
    36721 tcaggccgca tgggacggaa tagccgcaac gcgggtgttc cgtcccgtgt ggctcactgg
    36781 gggtttgggg cgagtagcgc cccatcgcgg ccaaaggccg caacgccccc ggcagggcgc
    36841 acacaccggc aacggtgcat aagtgcgccc ttgtcccaag ggagctatcc cgcgtccccg
    36901 gttttcgctg attttgcggc gacttctccg gctcccggca gacgggagag gacaagcagg
    36961 aacgccccca cttcctcgcc ggtcatggcg gccacggcgg gaaagacgct ttccagaatg
    37021 gccccgcgct caatgaggcg gtgcgtccgg gccttgcgct cggcgttggc tttcttgttg
    37081 agtagaatct tttggcggtt ttggagctgg tgcagctccg tttcaatctt tcgtttttct
    37141 tccagcaggt tcatagtccc tccctcgtcc acttcctttt tttgaaatac ttttgcagct
    37201 tcttcacggc ccggcgcacg gaatcgctgg cctgcgttgg gctgattccc tcggcctcgg
    37261 caatctcccg gtatttcatt tccagcatat agcgcatatg caggcggcgg gcctgcgtgg
    37321 gggtaagctg gccgatggcc tcccacaaat gctccaacaa tagctcttgc gccgcctcgt
    37381 cctcggcctg tatcatgtag tcctccgggg acggctgtgc ccagccgata gcgtcattct
    37441 caataccgtc ctcgcaatca agggagaaat atgccttgtg ataccatatc ttgctggtgc
    37501 ggttgttttc ctcccggcgt agatggagca gggcgtccgc tatctcgtcc ggcacttcca
    37561 cgaaaacgtc cgtcttgaca aggggatagt agtagcgttt cagattgatt actgtcatgg
    37621 gctgacctcc taaaatggca agaggggcag gcggtgaagc ctgtccctct cggtgatggg
    37681 gcgaatgtcc ctcacttggt tagcccgaaa atcgcgtttc gttagggtgg tctttgaaaa
    37741 gtttttgata tttttttctg actgccgcaa tgctctcata gacagatact ttcgagcagc
    37801 cgtccagccg ggcaatctgt tcataggtca aaccgtccag cgcatagagc aggaaccgcc
    37861 gccgctgtgc ctcggtgcag ctctccaaca cggaaagcag ctcgtcccgc gtttccttgc
    37921 ggcagacata ttcctccggt gtttcctgat ggggcgggcg gccctcggtg gcaactatgt
    37981 actcgtcaaa ctcccgtatg tcccaatggc ggcgcttctc gtgcgctaga ttttcctctt
    38041 tgtggtctgc ccggtctaag cactcgtaga cctcgacgct gacctccacg gacagggctt
    38101 gtccgtttat tttgatggtg acttccatct gcctatcctc cgtttcgatt tgggtttgtg
    38161 cgaatcgaaa cgggggcggc ggggaacggc agcggggccg aacatcgggc caacttggcc
    38221 cgaacctgac cggctggaaa aaggaattgt ccggccctcc tgtgccgaat tttattatct
    38281 tttcataaac tagcggcccg acaaaaacag aggcgcggaa tctctgcaaa tatccccggc
    38341 aatcggctgc aatcgcagac gaaccacgac ggaatagctc tggtcttgtc gcataaaaag
    38401 agggcgcaag tgattggttt ttcacttgcg ccctgttgga cgtttccact attctgttgt
    38461 ggtttgtccc ccaattaggg ggtggggcaa aagggatatt tgctcatggc ggccctccac
    38521 tggccgcaaa aaagcggcct tgatttctca aagccgccta aaaatcccat agaactctgc
    38581 tgctgtacga cgactttttt tattagccgt cgtgcggcag gttactatta cttatatttc
    38641 attctagcaa cactaatcat tttaatctta gttgggaaaa gttgtagtaa tctgtttttc
    38701 aaaccgggaa taattatctc cttatttttc attaaacccg aaaaggcaat ttgagcaacc
    38761 ttttcagcgg tcatagaatt tgtgggagta accgttccct ctcgaacgaa aaagtttgtc
    38821 ctagttgctc cggggcaaag tgttgaaatt tggacacctt ttgattttgc ctcatatctt
    38881 atagctctgg aatagctcaa tacaaacgct ttacttgcaa aataggttga agtatatgga
    38941 ccgggttgaa atgcacctgt cgaagcaaca tttagtattt tcccttgacc gcatttatac
    39001 atctccgcga taaacaattt agttaatatt acaagattca acgtgttaat tattatcatc
    39061 tgttcgtctt gcttcaagtc gatttcattt gttgcgccaa caagcccaaa acctgcattg
    39121 ttcactagaa ctgtaacagg tacttgttct tttgttactt tctgataaac ctcatatgct
    39181 gccccaattt gagttaaatc ctgttcaata acatagatag gtgtagaata atgtccttgc
    39241 agttttttct tgacattatc taaatgatct tgacttgatg atactaggat taggccatat
    39301 tcgtttttgg caaataactt tgccaactca tatccaattc cgcttgtcgc tccggtaatt
    39361 aaagcgaatt tcataaaaat tcctcctttt gagaatatta tagctctgca aaaggaggtt
    39421 gtaattcaca tgggttaatt tatatctggt tttttagttt ttttctaatt ctactcatag
    39481 aacctgcttt tatgccaata aacgacgcaa taaatttcaa cggtattcta cgttggagat
    39541 taggatactc tttacaaaaa cttatgtatc tttcatcagc agaaaggagc agtaagtatt
    39601 tggctctgct ttccaatttc cccaattcct ctaagtagag attttgaaca tattgctcca
    39661 cttgtttatc catagaaata atttttctta tcaccgtata tggtaacttt atacacttac
    39721 attcttcaat acattctaca taaaatgacg aacaagaatt ggttctaaaa cattctgaac
    39781 caaaaaaacc gttttcaaaa ctaaaacact ttgtagcctc ttttccttgg tcatctatgt
    39841 aatagcctct aacaattcca taaattataa aatagaatga tgacacttca tcacccattt
    39901 gtatcggatg gtagcccttt tcatatgtaa caacggaggc tttattatct atcacaacac
    39961 taatatcttt atccagtcta atcttcaaac aattttttag atattcactt ggctgtataa
    40021 tcataacggc cctccattat attttccttg ttacggctca tcatctggga taagtgcaat
    40081 tacatcggca atgtcacaat tcagagtttc acagatttta accagtgtag ccatagagat
    40141 atgttcaccc ttgcccatgt tggcgatatg gtttgtagtc aaatgcgctt gcaggcgtaa
    40201 atctcgcttg ctcatattct tgtctatcag tgttttccat agcggcttat aactgatttt
    40261 catagtggtt cctcaccttt ccggccagaa tatccgtaaa tccggccctg cctcctgtca
    40321 ttacagacat tataccacct taattgacaa aacacaatgt tagcttgcaa tcatactcct
    40381 taaactgcgg catacacaac tgttttattt gtgttttctt gtacctctat tttggtatca
    40441 agtgtttaat gccctgtgat tttgcacccg gattgtcgtt tccatatccc tctaagagat
    40501 tgataacgcc ccaaatgcca agtcctgctc caagagcgat aaccaaagtc tgtaatacgc
    40561 cgattgcact gttgaaaaat tccataaaac tcctttctgc cgcaaatgcg gcttgccccg
    40621 taaaagggca taaaaaacgg cggtcagttt tcaaactgcc gcggtcataa gtccccgcgc
    40681 tgcgtaaagt tctgccgcgc ggcggtattc agttgtcagt gtgggcgata ggaatttttg
    40741 taggggcgcg tatcatgcaa gttgtctttt tctttgttac tgcgtcaatt cctcctttct
    40801 tttcgctgct gctcatacag ggcttcacac cgtttcacaa actgccggat agcagcctgc
    40861 cgctgttttt cctgccactc ggcaatcgaa accagtccgt ttatggcttc tgtgattttc
    40921 ttgtcctttt tctttctgat acgtctattc atggtcggtg tcctcctgca aatcgctttc
    40981 gctgatttcg tagtagtcaa atacttcgtc cggcttcaca agggcgggtc tgcgccttaa
    41041 atgcttctcc atatcaaaag tatttttctt gtcatagtcg gaaagatatt tatagttggg
    41101 gtgctttgta atgtcgtact tatcagagaa gaacgggcgc acccctcgta gctgtaaaat
    41161 acatttcccg ccgtccataa ccgctatttc atcttccgtc ataagctgct tgcctaactt
    41221 ctgatagttc agcccatgcg atacctcgcg cccccggttc tcggaagtgt tgaagctgtc
    41281 aatggtttct ttccccaaga tttccgacgt ttctttgagg gtggttttct ccttgccgcc
    41341 caagaaaagg gtggtgtcgc agttgccgac tatggtatcg gcgttgtcct tatagattgc
    41401 ctttaactgg ctttgcgact gtaagataat cgacgcggag atttccctgc ttcgtatggt
    41461 ggctatgagc ttttcaaact tcggtatctg tccgatattc gcaaactcgt ctaacagaca
    41521 gcgcacatgg acgggcagcc tgccgccgta ttcatcatct gccttgtcgc aaagcagatt
    41581 gaaaagctgc gtgtaaagga ttgatacgac aaagttaaag gtatcgtcgg tgtcggaaat
    41641 gataacaaac agggcagtct ttcggtcgcc tatggtgtca agctccattt cgtcggtttc
    41701 cataaggtcg cgcagctcct taatgtcaaa aggggcaagc cgcgcaccgc aggaaatgag
    41761 gatagagctt cgtgtttttc ccgcagataa caagaatttc ttatactgcc ggacggcaaa
    41821 gtgttccggg tctttttcct ccaaccgttc aaacatgagg tcaacggggg actgaaattc
    41881 cgggtcgtcc tcgcgggctt cactggcatt tatcatttca agcagcgtcg taaagttctt
    41941 ttcttcctcc ggggcttcgt accaaatgta gccgataagg gcgcagtaaa agagccgttc
    42001 cgattttacc caaaaatcct ccccggcttt ttccccgtcc cctttggtgt tggcaatcaa
    42061 ggtatttacc agtttcaaaa tgtctttttc gctgcggata taggcaaagg gattgtagcg
    42121 catggatttc ttgaaattga ttgtattcag cacctttatc cgatacccgc cgcgctgtaa
    42181 gagcttcccg cactcgacta aaaccgtccc tttcgggtcg gttacaacgt agctgctgtg
    42241 catttgcatt aagttgggct tcacaaaaaa tcttgtcttg ccgctgccgc ttcccccgat
    42301 aacaaggata tttttattcc ttgcatactt cggctgcttc gggcggctgt tcatggtaag
    42361 ccgttccgtc tgtgtcagca gcacattatt ttgaaatacc gggtcggtgt agggttttat
    42421 atcttcggcg ttgccccaac gggcagaacc gtattctatg ccttttcgat atttcttcgc
    42481 gttcttgcct ttgaagtaca ccataagccg gataaggaca gcccccacaa taccgataag
    42541 caaatcggca ggaaaaaaac tcggaagcgg gtttgcaaag gcagcggaaa agccgtctgc
    42601 aagggaaagc accttgccgg aaaggtcagc ccccggagag agccgcaccg cctgtccgac
    42661 tttatcaaag agatagacaa agagcagata gggggcattt aggataagca gctttttaag
    42721 ttccggcttc atagagatac ctccctgtcc tttttcttca ctttgtccct ctgtgcgttc
    42781 agagctgccg ccttgtcttt cagagtggaa agcagcttgc ggatagaggg ctttttattc
    42841 ctgcctaatt tctttgcggt aaattcccgg aacgcttcgg tcataacgtc aatatccttg
    42901 cccctaaaag acacaaggta ggtcggcggc ttgccgttct caatcttctt gacgttgtaa
    42961 tcaagcccgt ttttccttgc gatagggtca aaggctttga tattctggtc ggtaatctca
    43021 atgttggaaa ccttttctcc cggcttcata agctgccgca tagtgatttt tccatgcggg
    43081 gctttggtta gctgcccttt gcctttggca agcagggctt tcatagcctt ttgcagcact
    43141 tcggcggtca gcttagatgt tttgattgct aaagacacaa ctttttcgtt gacttcatct
    43201 tgcatgaaat ccctcctttc gtggttttgt tagggtggtg tgccgcttcg gggcataaaa
    43261 ataaggcgta catttttcat gccaccttta gatacgcacc cgcagcccgt tcaaatccga
    43321 agccctcaca cccacaagat accagttgtc cccgtactcc atgcgggtcg gcttccattt
    43381 gccgcgtaca aacacttcaa ggcaatcccc acaatgcaat cctccgtagt agtcgttaag
    43441 gtcaaagcga atgtcgtaac ggtctgctgt ttcatcaaaa atcaaagctc ctgttttctg
    43501 tgtcataata aaatcctcct ttggttttac aggctttcgc cctcattgta ggtgtaatcg
    43561 ggtatgccct tgcttcttgc cgactggata gtacgcccgc cgtacatatc gtgattgact
    43621 agggcggtgt aatagctatc aatggtgttt ggggcattga acagtaccgc tttcagatat
    43681 tgcttgatgt tgcggatttt ggtggtgttc tcactcatac aggcaaaaac aaactcaata
    43741 tgactgctgt tcagcttcat aaactttgct ttcacaagct ctgccggata atcgtccccg
    43801 gcgatacgga tagtctttcg tttggtgcag accgtttcaa ggataaggga cacaatctcg
    43861 tctaagcgtt ctttctcaaa atcagtgtgc ttgatgaaat gctcatactc gatattgtcc
    43921 ttgataattt cctcatacac tcggtatgcg tctgtcgttt cctttcgctt ccgttccggc
    43981 ggctgtgccg cgtcctctaa aggagaggga tttagggaat ggaaaggaat ggaatgggta
    44041 tttgatatat ctgtatttaa tttttctttt tttggtaagt tagtctttaa tagttcttta
    44101 tttaattgcg ttggattttc cgatgtcggg ttatccggtg tcggattttc caatatcggc
    44161 ttatccgctg ttggattttc caataccgga caatccaatg tcgggggctg tggctgctcg
    44221 taaatggtgt actcgatagc ggtcattttg cctttctcgt cgcgtccctg ccgccttgtg
    44281 atatatccgg ctttttcaag ctcccataca gcggtgcgga tagcgtcgat actttcccgg
    44341 ttgataaggg acagccctgc aagggtatag tcccaatctt cgggaagtga aagcatttgc
    44401 gacaacaagc cctttgcttt taaggaaagc tccttgtttc gtaaatggtg gttgctcata
    44461 accgtataac ctttattctt ttccacgcgg aaaactgcca tagcttcatc actcctttgg
    44521 ttggatttgt tacgcagtag aaacgcggtt ttgagggaaa aggtatcaac ccttgccttt
    44581 ctcattttca cgtcctaaga gccgcataaa tcaaggcttt tttgcccctt actgcgtaac
    44641 atgagggcat aaaaatagcg gcgttcctgt tctcctgtgt aggagtgaga aacgccgcct
    44701 tataggtggt gcttatttta ttttttcttt tcgacgtgcc gcgtaaggtg gtgtagcggt
    44761 ctatccggca gctccgcagg tgcttttgca tatcaaggcg caatccgtta aaagtagtaa
    44821 atgagtagta aaatcaatct gcaaacctgc gaatatgccc gtaaatcaag catttttcaa
    44881 gacaatcaac taatttaagt tgaaatacgt ttaatatatt aatttttcaa agcattaata
    44941 tattaaagtt ataaccaaaa ttgaagttat aatctgccaa atctatagcg tattaagtgc
    45001 gttatataac ttaatctttt atataaagag ttaatttttt aatttgtaaa gcagagtacg
    45061 tttaaaagat actatttaga tagtgatgta ctatctaatt ttaaaatatc tacaataggg
    45121 ttctcttgaa gacttgctat tgcttctgca acagcataaa catcttcatt taatgcctct
    45181 gcttttacat ttgcaaatga tttactttta acaactgcct tacccttatc atcaaaacca
    45241 gcatccaatt ttagttttat actagataaa ttttttgttg aaacaacttc cataaaatta
    45301 cctcctaaaa atttaatttc aaagatttgt cgactttctt tgttagctat aatactatca
    45361 tctaagataa tatcggagtt aaacagtaat ttctaaagat tttttgtgta aatttttata
    45421 ggaaaaattt tacttttttg atgatgaaaa agtttacaag ttatttgtga agatatgttc
    45481 ttagtgtttt gaaatagtat ttgtatatat tcatgcttag ttgagatagt tttgtatgga
    45541 ttttcatatg tgtagagtta aatgttaggt actaaagttt atgttaaatt aaatgttaaa
    45601 gcttatgtta aaaattttat taaatttaat ctgtaaataa aaaagttaaa agtccaatat
    45661 taacatttta cttttaaacc attaaaatct ttattaaagc taacaaagtt ataagttatg
    45721 agaaatttta ttaaagttaa tacaaaaatt aattttaaag ctaagaaatt ttttattaga
    45781 ttcaataaaa ctaataaata tagtttagtc aataatgtat taaataaaaa ctttgttaag
    45841 tctataaaaa tatcttaaag gaggtgtaac ttatgaagat aaaagtggag gatataccag
    45901 ttcaatttca tgcaatggta gaaataatcg gaattgataa gttcatagag gttgccaagt
    45961 tgtatggagg aacaaatact tatataccta cgtataaggg gctttttaga tatgctagaa
    46021 atagggatat agtaaaacaa tttaatggag tcaaccacaa tgaattggct attagatata
    46081 atatgtgtgt aagcaatatt aagcgaatac tcaatgaaaa tagtgtttaa atataataaa
    46141 ataatgttta aacataatga aaatagcctt gttaaacgta agagtaaaga caaagaagat
    46201 gttttataaa gtttttttag acaaattaac taaatttact acttattaat ttaaatacat
    46261 tgtatattct ataaaaaaat ttgttaaata ctggagatat attgagaata caaagaaaat
    46321 ggtcgaaaat atttgttggc taaaaaatag ataaaatgat acaattgctt gtataaaggg
    46381 gaaaaataaa taaataaact atataaataa agaaatagca attaatatga cataatatat
    46441 ttataaaatt aagaatataa atagatattt taaggtatta gttgttaaat taggaaatat
    46501 taaaaaaaat atatatttat atttaaacta ttttgttatt tatataaaaa tacttatcta
    46561 gttactagca attattagta gaaatggggg aaatagagat gaataaggga ttttacaaat
    46621 taaattcagt ggtattgaac tttgatatta agaatgatgt taacaagata agatttaatc
    46681 attatataat gagaaaagaa aacatacaaa gacttcaagg aactctgcca aacggacaat
    46741 tttatatgac agtgagaaga gctgctacag atttacaact ttctatttct actataagta
    46801 ggcttgtaaa agagtttttg gatttaggga taataagatt agtgtctaag ggtgtaaaag
    46861 gtaatcgttc tgtatatagt tatgtaagta tagagtttaa tggaagttta tttgatgata
    46921 gtaaatatat ggaacttgac tgtgtatgta atgatgaatt tattgataat agtggcatag
    46981 agtttgattg tgatagcaaa gataattctt ttaatggaga tataaatgta gaaactgtgc
    47041 attgcattaa tgagtatttc tctgatgatg atgtagagca taattataac aatttccata
    47101 acaaagatac tataaaggat aaagccattt ctaatattag cactatcaaa aaatatattg
    47161 atgaatcttt tgatacaaat aaatcagaaa attctaactt acaacaggag tcaataaatt
    47221 tcaactttaa tgatacatgc aatgaaatag ataaaagaac gaataatagt atagaaaata
    47281 atcaaaataa aaggcctatg agattgagtg acatcagtgt tttaagtgat aaaggtatga
    47341 caaataaaaa gaattacttt aatcataatc atggtataca aaaagggact aaatctgtaa
    47401 caaagaaaaa agaatcctta aataaattta ataaaaaaaa tatacagcaa agcattataa
    47461 atatgctaaa taaaaaatca ggtaagaatt ttaaaatcga ttctccaata agcataaaac
    47521 ttatcaatga aaggctaaag gaaggctatg tcttagaaga tttctacaaa gttattgagg
    47581 taaaagtggc taagtggaaa aataccataa tggaaatgta tataagacca gaaaccttgt
    47641 ttagctataa gtttgaatcc tatgtaaatg aaaacatatc tatgaaaagt caaagtaatg
    47701 ttcacccatg taaagaaggc aatagtaatg atagcagtaa ttatatacat tctgattata
    47761 caacatatga tggcagtgtg cgtgagagaa aaaaatatga gaatgtaaga tactggaatc
    47821 ctatatgtga gatttaatta gtttaagaat gtatttatga taaataagat attaatagag
    47881 aatagtgagc tatagcaaat gatatatcaa tagttggtag taagttatat taagcaagat
    47941 attactatca ggtagtgaat tgtattg
//
LOCUS       DBGJFF010000014        45672 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.014, whole genome shotgun sequence.
ACCESSION   DBGJFF010000014 DBGJFF010000000
VERSION     DBGJFF010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 45672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45672
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.014"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            263..607
                     /locus_tag="ACOIAF_000875"
     CDS             263..607
                     /locus_tag="ACOIAF_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HGZ2667898.1"
                     /translation="MNNKEKGDFGEKVAVNYLLSKGAKILEKNYRLKIGEIDIIAKME
                     DEIIFVEVKSRSNIKFGYPCESVSFKKRKKIIGVASYYIIKNNLNNTPIRFDVIEVYL
                     LEKRINHIMNAF"
     gene            638..2239
                     /locus_tag="ACOIAF_000876"
     CDS             638..2239
                     /locus_tag="ACOIAF_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HGZ2667899.1"
                     /translation="MLSIINSSNLVGIDSFLVKVEVDVSNGIPSFNIVGLPGKEIKEA
                     RERVKSAILNSGYKFPSTRIVVNLSPADIKKEGAFLDLSISIGLLRELIKKDENYIRE
                     SMFIGELSLDGKIRKVRGILPIIMGAKTQNIKRIFIPIENIKESLFVDEIDIIPIKSL
                     KECVDFLNEEIKVDKVRIMSFLDDKSRKENEELEKDNSYIDCKYTKINNEESKYDEDF
                     KDVKGNYFVKRSAEIAAAGNHNMFMIGPPGSGKTMIAKRVRTILPDISVEEMIEVSKV
                     YSILGMINETKGIIDKRPFRAPHHTTTKQSLIGGGMDARPGEIALAHRGILFLDEIAE
                     FDRKILETLRQPIEDGYVNISRVKYSAKYPCRVLLVAAMNPCPCGYYMSETECRCRSN
                     EIDRYINKISGPLLDRFDIFVEVNSIKYSDFNSLKQEESSQKIKRRVENARKIQINRF
                     KKDNIKNNSEIKAYNLFKYCKLEKEASKTAEMIFNKYNLSSRSYTKLLKMARTIADLE
                     ERDLINSQCIIEAFSFRKAYYSYFK"
     gene            2258..3358
                     /gene="dprA"
                     /locus_tag="ACOIAF_000877"
     CDS             2258..3358
                     /gene="dprA"
                     /locus_tag="ACOIAF_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HGZ2667900.1"
                     /translation="MEKRDTYLWLKSIGGITTKTIEIIENEIVNIEDIFDFSEKEIYN
                     LKNISLNIRKNIVKYRGHAYLENIKELLYKKAIKYICKYDKEYPENLKNIYNAPNLLF
                     YKGDIGLVNNNFNIAIVGSRKPTAYGINCAKTISCQLSQYGVNIVSGLAIGIDAYSHI
                     GCMSGKSKTIAVLGSGVDNPLPKQNLHLSNKILENGGLLLSEYNINSTVAPYHFSNRN
                     RIISGLSDGVVVVEAAIKSGALITVDFALEHGKNVFAIPGNINSQMSRGCHKIIKEGA
                     KLIENIDDILNEYNIFNIIDKKINQKYDNISLNAKSKQIIEAIKREGNLHIDSICDYT
                     GIEIKYVNSIINELVLNELVVEMNNKTYSLNV"
     gene            3391..5478
                     /gene="topA"
                     /locus_tag="ACOIAF_000878"
     CDS             3391..5478
                     /gene="topA"
                     /locus_tag="ACOIAF_000878"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HGZ2667901.1"
                     /translation="MAKTLVIVESPAKAKTIEKFLGKSHYTVKASVGHVRDLPKSKLG
                     VDIENNFEPQYINIRGKGDVIKELKKEAKKSKQVYLATDPDREGEAISWHLAHILNLD
                     ESEDCRIEFNEITKDAIKKAIKHPRNIDINLVDAQQARRVLDRLLGYQISPILWQKVR
                     KGLSAGRVQSVTTKLICDREKEIKAFIPKEYWTIDVEAKTVKDKDITLKFYGKNDEKI
                     ELENEEAVNEILKEMEGKDLKVEKIESKSRRKSAPKPFTTSMLQQEGANKLFFTTKKT
                     MMIAQELYEGIDIEGEGTVGLISYIRTDSKRISDEAKEKAKGYIVNNLGENYYKNTGD
                     KKEKKDTKKVQDAHEAIRPTSVDRTPESIKASLSKDQYKLYNLIWRRFVASQMEDSVF
                     DILNVECKIGDIVFKATGSKMKFDGYTKVYNFSEREDKILPSIEKGDILNIENILPDQ
                     HFTQPPARYTEASLVKTLEELGIGRPSTYAPTIATILNREYVEKQGTSLCPTELGIIV
                     TDILENNFHKFIDVDFTADMESKLDVIEEGNTDWKKIVSESYAPLKEAIEEALENIEK
                     VNMDEETDEICENCGSNMVIKYGRFGKFMACKNYPDCKTTRPIMNKIGVKCPKCEEGD
                     IILRKSKRGKAFYGCSNYPNCDFVAWNKPTGEFCEKCGSYMVEKVTKSETKTVCSNKE
                     CNKETKEHNDGKE"
     gene            5643..6428
                     /gene="codY"
                     /locus_tag="ACOIAF_000879"
     CDS             5643..6428
                     /gene="codY"
                     /locus_tag="ACOIAF_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HGZ2667902.1"
                     /translation="MASEVLQKTRKINKTLQTSGGSSVSFDLLAGALGDVLSSNVYVV
                     SAKGKVLGLHLNDVQDSSVIEDEYTKQKKFSDEYTQNVLKIDETLENLNGEKILEIFP
                     EEHGRLQKYTTVVPILGSGQRLGTLVLSRYSNSFNDDDLVIAEYSATVVGLEILRAIG
                     EELEEEMRKKAVVQMAIGTLSYSELEAVEHIFAELDGKEGLLVASKIADRVGITRSVI
                     VNALRKFESAGVIESRSLGMKGTHIRILNDKLTDELKKLKNNQ"
     gene            6535..7800
                     /locus_tag="ACOIAF_000880"
     CDS             6535..7800
                     /locus_tag="ACOIAF_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HGZ2667903.1"
                     /translation="MPLADKIRPKTMNDVIGQSHIIGNGKILSKILQSNFLPNMIFFG
                     PPGVGKTTVAEIIAERSNKSFYKINATNSSLEDIKKVISELGSINNVNGVLLYIDEIQ
                     SFNKKQQQSILEFMENGSITLIASTTENPYHYVYKALLSRSTVFEFKPLEKLDVEKGL
                     KRAVEVLNEDSYMDIECNNDAIEDIAILSDGDMRRALNILEVVVYSTKPNKDNITYID
                     SDVIRASTFNKIINYDKNGDSHYDILSAFQKSIRGSDPQASIHYLARLIKGGDLISIC
                     RRLLVIASEDIGLAYPNAIVIVKACVDSAMQLGFPEAKIPLAEATILLATSPKSNSAC
                     IAIMKAMDELDKEFVKDIPNSIKDAHYSGSKEIGRGCGYKYPHNFKNHYVKQQYLPDS
                     IKDSIYYIPQENKTEKNIKKYLEYLNSDF"
     gene            7831..8601
                     /locus_tag="ACOIAF_000881"
     CDS             7831..8601
                     /locus_tag="ACOIAF_000881"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGZ2667904.1"
                     /translation="MIIKLNSTYHSRVMKYLKKEPEYNLFIIGDIERYGYGNNFLNIW
                     ADVGEHGEIKAILLKYFEFMMFYSDGEYDVEGFYNLLRNTNYEEISGKICAVDALAKR
                     LGLNNLKVVDFCKLQTKKFLIDNNCNAKVKRIRLGNLKKTVKLYDLIDEFHSTTLENL
                     KNGLRTGRGYCIEINKQVVSMAKSTSENRTHAMIIGVGTHPKYRAKGLATKCLIKLCS
                     ELLRENKIPCLFYDNEEAGKIYKKLGFENIGKWGIYSK"
     gene            8710..9156
                     /locus_tag="ACOIAF_000882"
     CDS             8710..9156
                     /locus_tag="ACOIAF_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HGZ2667905.1"
                     /translation="MKLSTKGRYGLKAMFELALNQDNGPVSLKFIAKKQKISDQYLEQ
                     IFSSLKKSGLVKSVRGAQGGYLLSKNAEDITVGDILVVLEGPVALSDCVLDEDVCENS
                     NMCVTKIVWEKMKKGIEDVIDSITLKDMINDYNKNKLENDITNIKK"
     gene            9174..10367
                     /gene="nifS"
                     /locus_tag="ACOIAF_000883"
     CDS             9174..10367
                     /gene="nifS"
                     /locus_tag="ACOIAF_000883"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HGZ2667906.1"
                     /translation="MEKRRLYMDYSATTPIKKEVLDAMMPYLTDYFGNASSFHTFGRE
                     AKDALDKAREQVAALINAEPSEIYFTAGGSESDNWTLEGVAYANKNKGNHIITSKIEH
                     HAILHTCEYLAKHHGFEITYLDVDSEGKVDLKQLEESIKDTTILISIMFANNEIGTIQ
                     PIKEISEIAKKHKILFHTDAVQATGNIPVDVKELGIDLMSMSSHKIYGPKGVGALYIR
                     KGVRLHNFVHGGAQEKSKRAGTENIPAIVGYGKAAELAKENMQNHVETLTRLRNKLID
                     GVLERIPYTRVNGSLENRLPGNANFAFQFIEGEGILLLLDMLGIAGSSGSACTSGSLD
                     PSHVLLAIGLPHEIAHGSLRLTVGDFTTDDDIDYILENLPKVIERLRSMSPLYDDAKK
                     QGLVK"
     gene            10421..10861
                     /gene="nifU"
                     /locus_tag="ACOIAF_000884"
     CDS             10421..10861
                     /gene="nifU"
                     /locus_tag="ACOIAF_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HGZ2667907.1"
                     /translation="MQYSDKVMEHFMNPRNMGEIDNASGVGEVGNPTCGDIMKIFLDI
                     DGDVIKDVKFKTFGCGSAIASSSMATEMIKGKTIKDALELTNKAVAEALDGLPPVKMH
                     CSVLAEQAVKAALIDYAQKNNIHIPELDGYVIDDAHDHDVEEEE"
     gene            11150..12229
                     /gene="mnmA"
                     /locus_tag="ACOIAF_000885"
     CDS             11150..12229
                     /gene="mnmA"
                     /locus_tag="ACOIAF_000885"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HGZ2667908.1"
                     /translation="MNKKVMIGMSGGVDSSVAAYLLKQQGYDVIGVTMKLWQDDDVVE
                     IEGGCCSLSAVEDARRVANKIGIPFYVLNFREVFKEKVIDYFIDEYLEGKTPNPCIAC
                     NKHIKFDDFYKKARQIGCDYVATGHYAKIEKDESTGRYLLKKSVTDKKDQTYALYNLT
                     QEQLEHTLLPIGDYEKDRVREIAKEMGMAVHNKPDSQEICFVKDNDYANYVKKHSKKR
                     IEEGFFVDTKGNILGKHKGILYYTIGQRKGLGITFGKPMFVIDINPINNTIVLGDNED
                     LFKKELIAKDVNFISIDTLEEPLRVQAKIRYSAKPSPATIHRVGEDTIKIVFDEAQRA
                     ITKGQSVVMYDGDIVVGGGIIEKSL"
     misc_binding    12372..12610
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            12678..15317
                     /gene="alaS"
                     /locus_tag="ACOIAF_000886"
     CDS             12678..15317
                     /gene="alaS"
                     /locus_tag="ACOIAF_000886"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HGZ2667909.1"
                     /translation="MEKMGLNEIRGKFLEFFESKGHYVANSYSLVPNNDKSLLLINSG
                     MAPLKNYFSGVEVPPSVRMCTSQKCIRTGDIENVGITARHATFFEMMGNFSFGDYFKR
                     ESIKWGWEFVTEWLNIPEDKIWVTVYEEDDDSYDIWAKEMNFPEERMVRLGKDDNFWE
                     IGTGPCGPCSEIYFDRGEEYGCDNPDCKPGCDCDRYLEFWNHVFTQFDRDEEGNYSLL
                     ENKNIDTGMGLERMGCIMQGVDTIFEVDTIKSILEAVEKLTGVKYGENPKNDISIRII
                     TDHIRAVTFLVSDGVLPSNEGRGYVLRRLLRRAARHGKLLGVKELFLQKLIDEVIKVN
                     DKAYPVLVEKESYIKKVVGIEEEKFNETIDQGTEILNSYIEVLKNEGKTVLSGQEAFK
                     LYDTYGFPIDLTKEILEEEHLSVDEEAFNEEMEKQKERARNARGNMDGESWKEDPLSK
                     LESTVDSIFNGYSEIYGEGTIEAIVKDDELVQSAEEGDKVSIVLDNTTFYPEGGGQVG
                     DCGLITNENLVLEVLNTKKGANNSIKHIGIIKSGRISNGDKVKTLVDRETRMSAARNH
                     SATHLLHKALREVLGEHVNQAGSLVTPERLRFDITHFEAISNEELKVIEEKVNNVILS
                     SLDIKCDIMNIKEAKEKGATALFGEKYGDEVRVVSMGDYSTELCGGTHLTNTSQVGMF
                     KILSEGGVAAGVRRIEAITGKAVYEYLKERDGIISEVCVNLKSKEDNLIQRISSLLEE
                     NKNLSKELHDMKAKMSLQSVDSIFDSKVEVNGVNLITNKFEGMDMDTLRETADNLRDK
                     LGSGVVVLANVVDDKVNFVVTATKDVLDKGIHSGNIVREVAKIAGGKGGGRPNMAQAG
                     ASDVSKVDQALSYASEVIKTQVK"
     gene            15342..15599
                     /locus_tag="ACOIAF_000887"
     CDS             15342..15599
                     /locus_tag="ACOIAF_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HGZ2667910.1"
                     /translation="MEKDLDYTMKFEGIPEDRMSVGDTIDFVYKALVEKGYNPINQII
                     GYLLSGDSSYITSHKNARAIIKKFERDEILEEVITHYLNRK"
     gene            15609..17780
                     /locus_tag="ACOIAF_000888"
     CDS             15609..17780
                     /locus_tag="ACOIAF_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667911.1"
                     /translation="MRKKIMSLFAIVIMIFVSTASYGFAEEKGKVIFIDMNRTNLGSM
                     MNIPILKNEIEKRGYVALMNIRGDQGTDDRRSYASMGAGTRASVTTEDYINFQKVDQD
                     TAKAFEVATGQKAKEINDLGINRSINENLEYGEYGANLGSLGKTLSENGLKTSVIGNA
                     DIIENNQLVKNRNLCLVAMDQYGRIDSGNIDNINKKDSKMPFGISTDYDKLLDETKKS
                     YKNSDAIFVELGDTYRLDMYRLNLNDNTYDTMKKNIVKNINKYLEQVFSMVGENDTVY
                     IASAFPSDLDYKNKRRLSPIIKFSGEGKGLLESATTRKAGIVANLDVGVDILGTFGLE
                     NKEMVGRSYTLVNKEDNISYLKSLYERIVSISSIRSTIINGFVGVISASWVIAMFAIF
                     FRNYMPHKERVFKVLKEFIKLGIIMPLSFMLAPLMNFKTPISISLGIFATTLILYLLG
                     RKLFKDNDIKHMGFFATLTILVIVIDSVFGTYLMQNNIMSYDAVIGARYYGVGNEYEG
                     VTIASAVFGLAVLLHYKKVSKLLVVIFSLIILITSAYPSMGANVGGAISECVAYLLFI
                     MLIYDIKIDFKKAVLLAVSAVVVVFAFAALDILSGSESHLGMFVQRIFLNGPGEILQT
                     FGRKIQMNMQLAQTSVWVNILLVGIGVIAVLIFKPSRHFRKIMNNFPILFKGFIASMV
                     GCIITLLVNDSGIVAASTASIYILIPLIIISINMIIFEKNN"
     gene            17986..18411
                     /gene="ruvX"
                     /locus_tag="ACOIAF_000889"
     CDS             17986..18411
                     /gene="ruvX"
                     /locus_tag="ACOIAF_000889"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HGZ2667912.1"
                     /translation="MLDGRIMGLDVGDKTIGVAVSDLMGLTAQGVKTIKRVGKKKDIE
                     ELKAIIKEKQVNKIVSGLPKNMNGTLGPQGEKVIKFCELVKTETGIDVEFWDERLSTV
                     AAERSLLEADVSRQKRKKVIDMLAAVIILQGYLDFKINS"
     gene            18489..18776
                     /locus_tag="ACOIAF_000890"
     CDS             18489..18776
                     /locus_tag="ACOIAF_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HGZ2667913.1"
                     /translation="MEENIINLIDENGVESQFEIILTLEAEGKEYAILMPLDDEEAEE
                     ALIFRIDEDEEGEILIPLESDEEYETVVAVYTAIMEEEGLNYDEDESNGLN"
     gene            19058..19516
                     /locus_tag="ACOIAF_000891"
     CDS             19058..19516
                     /locus_tag="ACOIAF_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HGZ2667914.1"
                     /translation="MANTMDLLKDKLKETGFKITPQRRAIVEILLKHDHSHLSSEEIY
                     DLVRVDCPEIGLATVYRTMQLLDEIGLISKLNLDDGCIRYEISLHKEDCHNHHHLICK
                     NCGKIMEAKEDLLDNIEKEIQSLYKFKILDHDVKFYGLCDECNGVSDSEE"
     gene            complement(19633..20370)
                     /locus_tag="ACOIAF_000892"
     CDS             complement(19633..20370)
                     /locus_tag="ACOIAF_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HGZ2667915.1"
                     /translation="MRGKTHCAIGILTAIQTSLIFKIPISLVDILVSATFAVLPDLDK
                     SNSMVSNFILKNNVSKYIYRIFIYAVNIIIFFISININDNFYLSAIITFVAIIIIEAK
                     LTHTFLRKVFLSLIFILLAICLYIIEVEIYFTIFCLMLSIFPWLKHRSFSHSIFATII
                     VYFLLKQIELITNINNLSFYGTIGYASHMFLGDLFTKQGIPIFYPLSEKKISLGFLTV
                     GGPFSNFIEKSFIFVLIGLIIFSILKL"
     gene            20587..22263
                     /locus_tag="ACOIAF_000893"
     CDS             20587..22263
                     /locus_tag="ACOIAF_000893"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HGZ2667916.1"
                     /translation="MQLFKKNTNKIKVMALGGLNEVGKNMTVVEYKDEIIVIDAGLSF
                     PEDEMLGVDIVIPDITYLVKNRDKIKGIFITHGHEDHIGALPYILKKINVPVYGARLS
                     IGLIQVKLKEHKMNNVKLNVIGPRQVIKLDNMEVEFLKNNHSIPDAYSIAIHTDQGII
                     YHTGDFKIDLTPIDGDVMDMHRICELSKKGVLLMLADSTNAEKPGFTMSEKTVGVGLD
                     ELFAKGNGRRIIVATFASNIHRLQQIINTAEKFNRKVAISGRSMVNVVGVAKELGYLD
                     ISDDMLIDLNDICKYEDSELVIITTGSQGEPMSALARMAFSEHKKVEIKSGDLVIISA
                     HPIPGNEKLISRVINFLFEKGAEVVYSDIADIHVSGHACQEELKLIHALVRPKFFMPA
                     HGEYRMLKRHAEIAEQLGMDKENIFVMQTGDVLELDKNSAKVANRIQTGNILVDGLGV
                     GDVGNIVLRDRKHLSEDGLMIVVVTISKDEGKVLAGPDIISRGFVYVRESEDLMDGAK
                     DIIKNVLNECEEKNIKEWAYLKNNIKENLKEYLYQKTKRNPMILPIIMEV"
     gene            22469..22669
                     /locus_tag="ACOIAF_000894"
     CDS             22469..22669
                     /locus_tag="ACOIAF_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1290 family small acid-soluble spore protein"
                     /protein_id="HGZ2667917.1"
                     /translation="MDDVSRQNAIKALKQTKMEIAGEYGMNYEDAFEIIENASNKGVL
                     EGYFKKLEKKKNLGQGISRHLE"
     gene            22723..23886
                     /locus_tag="ACOIAF_000895"
     CDS             22723..23886
                     /locus_tag="ACOIAF_000895"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HGZ2667918.1"
                     /translation="MKKLASTALAILIALTPLSFSFANNKENADANQLNISSKSAILM
                     DVGSGQILYEKDAHKKLPPASVTKVMTMLLIVEALDSGKIKLDDEVQVSETASSMGGS
                     QIFLEPGETQKVDTLLKGIAVASANDACVAMAEHLAGSVEGFVDRMNAKAKELNMNDT
                     HFANTNGLPVANHYTSAHDIALMSRELLKHEMISKYLTTWMDKVVVGKKQVTVGLANT
                     NKLIKHYQGATGVKTGFTQEAKYCLSASAKRGNTHLVAATLGAETSPERFNDASSLLN
                     YGFANYESVKLCSKGDNIATLTLDKADENKVKLVAKEDLNALIKKGSSKEFEKKIEIV
                     KNPKMPIKKGTVLGKIKICKDKKVIGEVELINTKDINKASYLQMLQRIIDNMI"
     gene            complement(24097..24519)
                     /locus_tag="ACOIAF_000896"
     CDS             complement(24097..24519)
                     /locus_tag="ACOIAF_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2000 domain-containing protein"
                     /protein_id="HGZ2667919.1"
                     /translation="MENKNSKCVMVIDENLPMGIISNTAAIMGITLGKHAPETVGPDV
                     IDKTGNSHLGIIDIPVPILKGNKEIIKDLRKKLYTLEFNDLTVVDFSDVAQSCNLYEE
                     FTQKIASVPEDELQYFGIAIYGNKKKVNKLTGSMPLLR"
     gene            complement(24540..25376)
                     /locus_tag="ACOIAF_000897"
     CDS             complement(24540..25376)
                     /locus_tag="ACOIAF_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HGZ2667920.1"
                     /translation="MRQEERTVCFDRELKIEAYRFKGIMQKFPNHFHEHYVIGFIEKG
                     QRYLSCKNKEYTTSTGDLLLFNPFDSHTCEQIDDKVLDYRCINIKPEIMKKTVFEITG
                     KNYLPKFNQPVIFRSELVPLLQELHYIIMEEELDFKKEELFFFLIEQLIEEHTEPNLQ
                     SNLENTNIEIQAVCDYLENNYAEHIVLDELSTIAGMNKYSLLRNFTKLKGITPYRYLE
                     NIRVNKAKKLLEKGVEPIDAAIQTGFVDQSHFTNFFKNFIGLTPKQYQNIFINDDSNN
                     FK"
     gene            25674..26270
                     /locus_tag="ACOIAF_000898"
     CDS             25674..26270
                     /locus_tag="ACOIAF_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HGZ2667921.1"
                     /translation="MLSMNIGEIVASLVGIAMAISIHEFGHAYSAHLLGDDTAKAYGR
                     MTLNPVRHVDPIGLLAMFILKIGWAKPVPVNPNNFKNYKIGNLIVSLAGVFCNVLTAI
                     LCVIINKYVHMYAINTIAGYVLLYSMGFAAFNLLPIPPLDGWGVISTFVPYKWNEYLY
                     KFESMSYIILLIALFTGIYQIILSPIYTVLLKIVYLFV"
     gene            26300..27019
                     /locus_tag="ACOIAF_000899"
     CDS             26300..27019
                     /locus_tag="ACOIAF_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HGZ2667922.1"
                     /translation="MKYNIQLQVYEGPLDLLYDLITKHKIDIKDISIIDITKQYLNYL
                     KMLDKMDLEITSEFITMASKLLEIKSKYLLYKQKDEEEDPRIELMEKLEEYRKFKVAS
                     QDIKENITYVNERFYRNKEEIIIDDNVDLEDISIEAIKNILPYIFKVKTSQIENANDE
                     KLDKIVRKKIISVEEKILYIRDIIKDKIEVTFTNIIKSYENDEIIATFLSILELIKEK
                     EIVVVQDIFFDDILIRKSSEC"
     gene            27021..27560
                     /gene="scpB"
                     /locus_tag="ACOIAF_000900"
     CDS             27021..27560
                     /gene="scpB"
                     /locus_tag="ACOIAF_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HGZ2667923.1"
                     /translation="MKREDIKYIIESVMFAYGEPISIKELNYIINKELSSKEIEIMLN
                     LLIEEYREQNRGIQIIKLENKYQMCTNKDYAEYIKKIIEPKKKKSLSQATLETLTIIA
                     YKQPITKVEIEDIRGVKCDKVLQTLFENELIREAGRLNKIGKPIIYKTTDEFLKLLNI
                     ESLEELPPIENYQEVATNE"
     gene            27722..28351
                     /locus_tag="ACOIAF_000901"
     CDS             27722..28351
                     /locus_tag="ACOIAF_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HGZ2667924.1"
                     /translation="MELNELFNFKYLIIAVIFIISVTMLILISHINILIIADIKNKDI
                     CLKLNIKYMFNLININRQLYPAENSKNNDKKEGMKNNIDSSILLADDLLSIYRLLKKI
                     KIHELYSNINFGTGNIGLTSSVYVLINTLYGNLFNMIDAEKMYLNVNPDFTKDYVLGN
                     IRIHIRPRIKALFNIIIMINKIMNKNKGNKEGDSNESNRFDTESYGNNS"
     gene            28305..28733
                     /gene="ytfJ"
                     /locus_tag="ACOIAF_000902"
     CDS             28305..28733
                     /gene="ytfJ"
                     /locus_tag="ACOIAF_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GerW family sporulation protein"
                     /protein_id="HGZ2667925.1"
                     /translation="MRATGSIQSLMETTLETIKGSIDANTIIGDPIKTDTTVVVPISK
                     VTIGFGIGGGEYSKGYDDKDREIELKNEKSDTNFAGGSAGAISVQPVAFVVVESGETR
                     IMSLDSNINLVDNILSITPRVLEKIQNISQSNKNQDKNNM"
     gene            28981..29421
                     /locus_tag="ACOIAF_000903"
     CDS             28981..29421
                     /locus_tag="ACOIAF_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HGZ2667926.1"
                     /translation="MSDKELSILLLESMNNFHNLIKIINNERYKKDKVLTERQFFALV
                     KIRKHDKIELKNLSRDLHVSTSSLCILLNKLVEQEYVYREEDSRDRRNTFYGITKNGE
                     KILDNEILKFVSIISDKMDCLDIDNKDKLFTSLEESKNIIEQLF"
     gene            29437..30888
                     /locus_tag="ACOIAF_000904"
     CDS             29437..30888
                     /locus_tag="ACOIAF_000904"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HGZ2667927.1"
                     /translation="MNIKYSKEILKSLENAITVVALFEGEDVSTTFDKINMISRNFNS
                     KENSIDTVTLIENNNLVNVCLVGFGKRELLNREKIRIIGGNLCKKLKNILKNEAYKSS
                     DVNVLNLNLNSDEIGCFVEGILLGNYKFDKYKTKSKDFERKEVDTITIFTEKEVECQI
                     EKAKILANSTIIARNLVNEPSNVIYPKTLAVETVKLGAEFGFNVDVYEEEKIKALGMD
                     AFFAVSRGSINKPRFIVMRYFGDKESTDILGLVGKGLTYDTGGYSLKSNASMLDMKTD
                     MAGAASVIGAMCAISQSKLKKNVIAVVAACENALSGGSYKPGDIISSMAKKTIEVLNT
                     DAEGRLTLADAIYYIINNEKVTKVVDVATLTGAALTLLGNVATPIVTNNDDFYCELEK
                     AATLSGERVWKMPIYDEFKDMIKGEEADLKNTGGKNAGCITAGAFIGEFVGNTPWIHM
                     DIAGTSTSSKSIGYKAKGATGEPVRTLYYLAEI"
     gene            complement(30979..31647)
                     /locus_tag="ACOIAF_000905"
     CDS             complement(30979..31647)
                     /locus_tag="ACOIAF_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HGZ2667928.1"
                     /translation="MFKIFLFSSEQFVSLFIFGLFLYYCPKLTKNILPYSYTVEKIIC
                     TLLVIIMALEQLLLISSGNYSTLNSLPIGINYICIYLCIAILIFKQYHLFNIFFSWSL
                     VCSVGELIFSKNLGYEFPSLIYFIFILSKCLIIYADIYMVDVRKFRVNRYALRDNLAI
                     CFIYFSFIFLLNTFTNSRYYYGFLSHSTTAIFTFIFVTSIMYIPALLFNRDTFILEKK
                     KKSK"
     gene            31916..32056
                     /locus_tag="ACOIAF_000906"
     CDS             31916..32056
                     /locus_tag="ACOIAF_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667929.1"
                     /translation="MGIIESASKLAEMVHLLAVEKGITDIEAWDEAVKEYSKIYEERR
                     NE"
     gene            complement(32231..32551)
                     /locus_tag="ACOIAF_000907"
     CDS             complement(32231..32551)
                     /locus_tag="ACOIAF_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HGZ2667930.1"
                     /translation="MKLKTISLPELNNLDPTLESTFIKMGEEQGELAECIGKFRNLSG
                     ENNDLDEVDIIKKTAKELMDVAQTCVTMMFKLEEQYGINLDEIRKEHIKKLEKRGYIK
                     NMDK"
     gene            complement(32671..33408)
                     /locus_tag="ACOIAF_000908"
     CDS             complement(32671..33408)
                     /locus_tag="ACOIAF_000908"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HGZ2667931.1"
                     /translation="MDANSLKDLIANHNNIVFFGGAGVSTESNIPDFRSSTGLFSQKL
                     NKQFTAEQLVSHTFFVRYPEEFFEFYKDKLIYPNAKPNNAHIALAKLEEMGKLKAVIT
                     QNIDGLHQMAGSKNVLELHGSVHRNYCTKCGKFFDLESMLNLGGNIPYCDNCGSIVKP
                     DVVLYEEALDSDVITKTISAISNADLLIIGGTSLAVYPAASFIDYYKGDYIALINKAN
                     TVYDKSASLVINKPIGEVLYEAVLRQI"
     gene            33623..35446
                     /gene="acd"
                     /locus_tag="ACOIAF_000909"
     CDS             33623..35446
                     /gene="acd"
                     /locus_tag="ACOIAF_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HGZ2667932.1"
                     /translation="MKKKAALATLAMLPLGVVNAHADGDIGIVTINYLNVRNEPTAES
                     SIAFVAKKDDKVLIKDSSNGWYKIKAESGQEGWASSKYIAKSNSDSLRTSTNKEKQVI
                     SNSLNMRNGAGTSYRVITVLKKGQKVEVISESNGWSKIKYDGRLGYVSSSYLGDVSNS
                     TNKSKTKQVNTTSLNVRSGPNTSYGLLGKLPKGSKVEVISESNGWSKIKYNGKDAYVS
                     SMYLSDVSQSNSDNSSQSNDKKNTDKVVNTASLNVRSGPGSTYSKLGKVYKGSKVTVL
                     SESSGWAKINFNNKEAFVVGNYLSTSADTSNNNSNSNSDNSSNSNGNNSSSSGQVNGM
                     SGISGAKIDYKSLSYTLESHISKQVEKAASGGNVIAPSNRKSTPSPEFSTFSAQRTSS
                     FVNASSSDIEYYLNPKNFTNTTKGMMQFLKINSYRDGISESSLNSYLNGLSSSVFKNQ
                     GAAFINAAKKYNIDVVYLVSHAMWETAYGKSTLAQGQTLTSYKGQPLSKPVKVYNFFG
                     IGAIDKSANVSGAEAAYSNGWTSVEATIDGSAKWISQNYVNSSKYNQNTIYKMKWNYD
                     YTWHQYATDVNWANGISGIMENLIGLYGGGSSLVFEVPQYK"
     gene            35661..39959
                     /locus_tag="ACOIAF_000910"
     CDS             35661..39959
                     /locus_tag="ACOIAF_000910"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HGZ2667933.1"
                     /translation="MESIKEYLDKLEINNSGLGKQLKEVYINRVVYFKEDKIVYFYLT
                     SKDIVSHELLDKFKEELMYKLDYFKDMRMKIRFTGLERKSNKDVIKKYWNNILYILKY
                     LCPSIAGWYKQVEFLCLEEELKIKLPKGIFYERLMKKNVTYVLKTVLSEELGLDLNIT
                     IEKAVDEKVNKERLIRINDREMEEKIRALEIGKVNNCEENEEESYVIKSDVDENLIYG
                     DNANAMIENIVELNASSGTVAVVGDIFDVDTKELKNGKILMIASITDYTSSISCKLFL
                     TDTNKDGVLENVKKGAYLKIKGDIVYDTYQREISMMISGIRKETRIEREDKSEEKRVE
                     LHAHTQMSSMDAICSVKKLVERAAKWGHSAIAITDHGVVQGFPDAMNAGKSNNIKILY
                     GVEGYLVEDDSLIIEDANDKELSQTFVVFDIETTGFSNTNDKITEIGAVKIENFKIVD
                     RFSELINPEKDISYKIQELTGITNELIKDKPTIEEVLPKFMEFVGDSVLVAHNAEFDT
                     GFISQKCREQGLLYNNKKVDTLMLARVMLPNLKRHRLNVVAKELGIPLLNHHRAVDDA
                     EATALIFNKFLQMLTEKGAKTLSDVNNILGKIDYTKLSTNHITLIAKNSIGIKNLYKI
                     ISDAHVNHFFRAPRILRSVLNEYKEGLIIGSACEAGVVFQAVKKNVSDEEMKKIIDLY
                     DYIEVMPIDNNRFMIDKGEVKDEEELRELNRKLIDTAKKFDKIPVATGDVHFLDKHEA
                     VLRKVLKYSQGFKVDEEETYLHFRTTDEMLEEFSYLGEELAYEVVVENSNLIADMVED
                     IKPIPDDTYPPIIEGSDVELREMCYKKAKRIYGDPIPEIVQKRLDRELNSIISNGYAV
                     MYIIAQKLVAKSLSDGYLVGSRGSVGSSFAATMSDITEVNPLPAHYICENEDCKYSFF
                     YEIGEWGSGVDLPDKDCPKCGRKLAKNGHDIPFEVFLGFEGDKEPDIDLNFSGNYQPT
                     IHKYTEELFGEGYVYRAGTIGTVAEKTAFGYARKYVEENNISVPNAEVLRLSNGCTGV
                     KRTSGQHPGGVMVIPDYKDVYDFTPIQYPANDTSCGVITTHFDYHSISGRILKLDILG
                     HDGPTIIRMLEDMTGINITEIPLDDKETMSLFTSTEALGVTPEEINCPIGCLAVPEFG
                     TKFVRQMLLDTKPTTFAELVRISGLSHGTDVWVNNAQDLVREDIVGLKDVISTRDDIM
                     NYLIFKGLPPKMSFTIMESVRKGKGLKPEHIEEMKKNNVPEWYIGSCKKIKYMFPKAH
                     AVAYVMTSFRIAYCKVHYPEAFYATYFTTKVEDFDADLIVKGLDAIKSKINEIESLGN
                     DATTKEKGMLTVLEVALEMYARGIKLLPVDIYKSDATEFIVVGEKTLLPPMAAIQGLG
                     GNAAINIQNERKNGEFISKEDLRKRTKISKTVIETLTNHGSLENMSERNQLSLF"
     gene            40163..40624
                     /gene="rimP"
                     /locus_tag="ACOIAF_000911"
     CDS             40163..40624
                     /gene="rimP"
                     /locus_tag="ACOIAF_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HGZ2667934.1"
                     /translation="MKKNLEATIEEIVTKITDEHGFEMVDVEYVKEAGEYYLRVYIDK
                     EEGISLNECELVSRELSPILDEKDPIKENYFLEVSSPGLDRALKKDRDFVRYQGRDVD
                     LKLYKPLNGCKQFEGELVGLTEDNNIKIIANGKEMEFNRKDVAIVRLAIKF"
     gene            40654..41808
                     /gene="nusA"
                     /locus_tag="ACOIAF_000912"
     CDS             40654..41808
                     /gene="nusA"
                     /locus_tag="ACOIAF_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HGZ2667935.1"
                     /translation="MNHEFMEALDELVVDRGIDKEILIDTIEQALLTAYKKNFGSAQN
                     VRVEFDRDKGDIKVFSQRVVVDESDLYDTFLEIELADAREINPNYELGDIIEHEVTPK
                     NFGRIAAQTAKQVVVQRIREAEREIVYNEFIEKENEIVTGEVARANKNVVYVNLGRIE
                     GIMTQSEQIPFENYKAGQKIKFYILEVKKTNKGPQIVVSRSHPGLVKRLFEEEVPEIY
                     EGIVQIKSVAREAGSRTKMAVRSIDEKIDPIGACVGPKGLRVKNIVDELGDEKIDIIK
                     YSEDPAEFISAALSPSKVVRVDIDEDEKSALVIVPDYQLSLAIGKEGQNARLAAKLTN
                     WKIDIKSESQAEQEMLNLENSKEIEAEDVEAEFIDQEELDTSEDNFSEEE"
     gene            41815..42096
                     /locus_tag="ACOIAF_000913"
     CDS             41815..42096
                     /locus_tag="ACOIAF_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HGZ2667936.1"
                     /translation="MILKKVKKVPQRKCIACQDRDSKKELIRIVKNKEGKIFLDPTGR
                     ANGRGAYVCKSSECLKKAIKSKALNKAFKIDVPDEVYDNLLMELEEYEK"
     gene            42086..42397
                     /locus_tag="ACOIAF_000914"
     CDS             42086..42397
                     /locus_tag="ACOIAF_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L7Ae/L30e/S12e/Gadd45 family ribosomal protein"
                     /protein_id="HGZ2667937.1"
                     /translation="MKNNKEKVLSFLGLATRAGKIVSGDDSTLLDLKKGKVNLILIAE
                     DASNNTKKLFKDKSTFRNIPYLFFSTKEEIGFAIGKSPRAVVGIKDENFSKKIIELIE
                     I"
     gene            42419..44359
                     /gene="infB"
                     /locus_tag="ACOIAF_000915"
     CDS             42419..44359
                     /gene="infB"
                     /locus_tag="ACOIAF_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HGZ2667938.1"
                     /translation="MSKTRVYQIAEELNISNEELINKLAELDINVTDKDSVLEGEELE
                     LALEMLGEDLSQENGNVIEIDGKLTVQVLATKLDKSPSEIIMKLMKMGTMATINQEIS
                     FEIAALAAKDYGFELTVAESDDTEALEIEALMEIEEDKEEDLKPRPPVVTVMGHVDHG
                     KTSLLDAIRKTDVISGEAGGITQHIGASEVKINGHKIVFLDTPGHEAFTSMRARGAQV
                     TDIAILVVAADDGIMPQTVEAINHAKAAGVPLIVAINKIDKPGANPDKVKQELADQGL
                     LVEDWGGEVIAVPVSAKKKEGIDTLLEMVLLVAEMEELRANPNKRAVGTVIEAELDKG
                     RGPVATVLVQGGTLTVGDPIVAGVACGKVRAMINAKGKRVKTAGPSTAVEILGLSEVP
                     QGGDQFVEVPTDKIARSVAARRQQIVRDEMLKSTQRLSLDALFSQMSEGSIKDLNIVI
                     KADVQGSVQAVKQSLEKLSNEEVQVKVIHGGVGAVTESDILLAAASNAIIIGFNVRPV
                     PGAESLGEKENVDIRTYTIIYKAIEDIQAAMTGMLDPEYVDEETGKAEIREIYKISGV
                     GTVAGCYVTNGKIFRNCKVRLVRDSIIIHEGELAALKRFKDDVKEVNSGYECGMSFVN
                     YNDIKEGDIVEAYITKEVERKL"
     gene            44402..44782
                     /gene="rbfA"
                     /locus_tag="ACOIAF_000916"
     CDS             44402..44782
                     /gene="rbfA"
                     /locus_tag="ACOIAF_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HGZ2667939.1"
                     /translation="MASYNRTRRIAEEIRKVVSTMLINGVKDPRITSMVSVTDVEVTN
                     DLRYAYVYVSILGGDEESTLTGLKSAGGYIRREVGKNIKLRYIPEIVFKLDDSIEKGM
                     YMDSLIKRVNEKNAQQNGDENYDE"
     gene            44763..45440
                     /locus_tag="ACOIAF_000917"
                     /pseudo
     CDS             44763..45440
                     /locus_tag="ACOIAF_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438308.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
BASE COUNT        18475 a         4849 c         8295 g        14053 t
ORIGIN      
        1 caacattttt caaatagctt acaactaata taaataaaga taaatatcgt ataaaagcaa
       61 ctaatttttc tctgttcatt aaaggcactc ctccaaaaaa ataaactttt cctatgaaaa
      121 ctagaataat atcataaaag aataaagtca atagaagtat ttattaatat tgatggaaat
      181 aaaatcaatc caaaaacttg aatagtaaaa ttgtatactg accataattt aaaagtgaaa
      241 atttaatgta ttggagtgaa taatgaataa taaagaaaaa ggtgattttg gagaaaaagt
      301 agcagttaat tatttgctat caaaaggagc aaagatacta gaaaaaaatt acagattaaa
      361 gataggggaa atagatataa tagcaaaaat ggaagatgaa attatatttg tggaggtgaa
      421 atcaaggtct aacataaagt ttggctaccc ttgtgaatct gtaagcttca aaaaaagaaa
      481 aaagataatt ggagttgcaa gttattatat tataaaaaat aacttaaata acactcccat
      541 aagatttgat gttatagagg tatatttatt agaaaaaaga ataaatcaca taatgaatgc
      601 tttttaaaaa agataatgtt tttggaaagg attaaatatg ttaagtatta ttaattcaag
      661 caacttagta ggaatcgaca gctttttagt taaggttgaa gtagatgtaa gcaatggaat
      721 acctagtttt aatattgttg gtcttccagg caaagaaatt aaagaagcaa gagaaagagt
      781 aaagtcagca attcttaata gtggatataa atttccaagc acgagaatag ttgtaaattt
      841 atcaccagca gatataaaaa aagaaggagc ttttttagat ttatctataa gtataggcct
      901 tttaagagag ttaataaaaa aagatgaaaa ttacataaga gaaagtatgt ttataggaga
      961 attatcatta gatgggaaaa taagaaaagt aagaggtata ttgcccataa ttatgggtgc
     1021 aaaaactcag aacataaaac gtatttttat accaattgag aacataaaag aaagtctatt
     1081 tgtagatgaa attgatataa ttcctattaa aagtttaaaa gaatgtgtcg attttttaaa
     1141 cgaagagata aaagtcgata aagtaaggat aatgtctttt ttggatgata agtcaagaaa
     1201 agagaacgaa gaattagaaa aagataatag ttatatagat tgcaaatata ctaagattaa
     1261 taatgaagaa agtaaatatg atgaagactt taaagatgta aaaggaaatt attttgtaaa
     1321 gaggagtgca gaaattgcag ctgctggaaa tcataatatg tttatgattg gacctccagg
     1381 ctcaggaaaa actatgattg caaaaagagt aaggacaatt ttaccagata taagtgtaga
     1441 agaaatgata gaagtaagta aggtttatag tatattgggt atgataaatg aaactaaagg
     1501 aataattgat aaaagacctt ttagagcacc tcatcataca actacaaagc aatctttaat
     1561 tggaggagga atggatgcaa gacctggaga aattgcttta gcacatagag gtatcttatt
     1621 tttagatgag atagccgaat ttgatagaaa aatacttgag actttaagac aacctataga
     1681 agatggatat gtaaatatat ccagagtaaa gtacagtgca aaatatcctt gtagagtact
     1741 gttagttgca gctatgaatc catgtccatg tggttattat atgtcagaga cagagtgtag
     1801 atgtagaagt aacgaaattg atagatacat aaataaaata tcaggtccat tgttagatag
     1861 gtttgatatt tttgtagaag ttaattctat aaaatattca gattttaatt ctttaaaaca
     1921 agaagaaagc tctcaaaaaa taaagagaag agtagaaaat gctagaaaaa tacagataaa
     1981 tagatttaag aaggataata taaaaaataa tagtgaaatt aaagcttaca atttatttaa
     2041 atattgtaaa ctggaaaaag aagcttcaaa aacggctgag atgatattta ataaatacaa
     2101 cctaagcagt agaagttata caaaactttt aaaaatggca agaacaatag cagatttaga
     2161 agaaagagac ttgataaatt cacagtgtat tatagaagct ttttcattta gaaaggctta
     2221 ttatagttat tttaaatagt tttttgaggt ggattttatg gaaaaaagag atacttattt
     2281 atggttaaaa tcaattggtg gaataacaac aaaaacaatt gaaataatag aaaatgaaat
     2341 agtaaatatt gaagatatat ttgatttttc tgaaaaagaa atatataatt taaaaaatat
     2401 aagcttgaat attagaaaaa atatagtaaa atatagaggt cacgcttatt tagaaaatat
     2461 aaaagaacta ctttacaaaa aagcgattaa atatatttgt aaatatgata aagaatatcc
     2521 tgaaaattta aagaatatat acaacgctcc aaatttatta ttctacaaag gtgacatagg
     2581 tttagttaat aataatttta atatagctat agtaggttca agaaaaccta cagcctatgg
     2641 aattaattgt gcaaaaacaa taagttgtca actatcccaa tatggagtta atatagtaag
     2701 tggattagct ataggaatag atgcatactc gcacataggt tgtatgagtg gaaaatctaa
     2761 aactatagca gtacttggtt caggagtaga caatcctctt ccaaaacaaa atttacattt
     2821 atcaaataaa atattagaaa atgggggatt attattatca gagtataata ttaactcgac
     2881 agtagccccg taccattttt ctaatagaaa tagaattatc agtggtttaa gtgatggagt
     2941 agttgttgta gaagcagcaa taaaaagtgg tgcactaatc acagtagatt ttgctcttga
     3001 acatggaaaa aatgtttttg caatacctgg taatataaat tctcaaatga gtagaggttg
     3061 tcataaaatt ataaaagaag gtgcaaaatt aattgaaaat atagatgata ttttaaatga
     3121 atataacata tttaatatta ttgataaaaa aataaatcaa aaatatgata atataagtct
     3181 aaatgccaag tctaagcaaa taattgaagc tataaaaaga gaaggaaact tgcatataga
     3241 tagtatttgt gattatactg gtatagaaat aaagtatgta aattcaataa tcaatgaatt
     3301 agtactaaat gagttagtag tagaaatgaa taataaaacg tacagtttaa atgtataaat
     3361 taagattagg tagtgggggt gtatggtttt atggccaaaa cattagtcat cgtagaatcg
     3421 cctgcaaaag ctaaaactat agaaaaattt ttagggaaaa gtcattacac agttaaagca
     3481 tcagttggtc atgtcagaga tttacctaaa agtaaattag gtgtagatat agaaaataat
     3541 tttgaacctc aatatataaa tataagagga aaaggcgatg taataaaaga attaaaaaaa
     3601 gaagctaaaa aatctaaaca agtatatcta gcaactgacc ctgatagaga aggtgaagct
     3661 atatcatggc atttagctca tatactaaat ttagatgaat ctgaggattg tagaatagag
     3721 tttaatgaga taactaagga tgcaattaaa aaagcaatta aacatcctag aaatattgat
     3781 ataaatttgg ttgatgcaca acaggcaaga agagtgctag acagactttt aggttatcaa
     3841 ataagtccta tattatggca aaaggttaga aaaggtctta gtgcaggtag agtgcaatct
     3901 gttacaacaa agttaatttg tgacagagaa aaagaaataa aggcatttat tccaaaagaa
     3961 tactggacaa tagatgttga ggctaagaca gtaaaagaca aagatataac tttgaaattt
     4021 tatggtaaaa atgatgaaaa gattgaatta gaaaatgaag aagctgtaaa tgaaatacta
     4081 aaagaaatgg aaggtaaaga tttaaaggtt gagaagatag aatctaaatc aagacgtaaa
     4141 tcagctccaa aaccatttac aacaagtatg cttcaacaag aaggagcaaa taagttattt
     4201 tttactacta aaaaaacaat gatgatagca caagaattat atgaaggtat cgacatagaa
     4261 ggtgaaggta ctgttggtct tatatcatat ataagaactg actctaaaag aatatctgat
     4321 gaagcaaaag aaaaagctaa gggctatatc gtaaacaatt taggagaaaa ctactataaa
     4381 aacactggtg ataaaaaaga aaaaaaggat actaaaaagg ttcaagatgc ccatgaggca
     4441 ataagaccaa cctcagttga tagaactcct gagagtataa aagcctcatt gagcaaagac
     4501 cagtacaagt tatacaattt aatttggaga agatttgtag ctagtcaaat ggaagattca
     4561 gtgtttgata tactaaatgt agaatgtaaa ataggcgata ttgtatttaa ggcaactggt
     4621 tcaaaaatga aatttgatgg ttataccaag gtatataatt tttcagaaag agaagacaaa
     4681 attttaccaa gtatagaaaa aggagatata ttaaatatag aaaatatatt gccagaccaa
     4741 cattttacac aaccaccagc aagatataca gaagctagtt tagtaaaaac actagaagaa
     4801 ctaggtatag gtagaccaag tacgtatgct ccaacaatag ctactatact gaatagagag
     4861 tatgttgaaa agcaaggaac aagtctatgt ccaacagaac ttggaataat agttactgat
     4921 attttggaaa ataatttcca taaatttata gatgtagatt ttactgctga tatggaaagt
     4981 aaacttgatg ttatagaaga aggcaataca gattggaaaa agattgtatc agaatcatat
     5041 gcaccactta aagaggctat tgaagaagct cttgaaaata tagaaaaagt aaatatggat
     5101 gaagaaactg atgagatttg cgagaactgt gggtctaata tggttatcaa atatggtaga
     5161 tttggaaagt ttatggcttg taaaaactat cccgattgta aaacaactag accaattatg
     5221 aataaaattg gtgttaaatg tccaaaatgt gaagaaggag atattatact tagaaaatct
     5281 aaaagaggta aagcttttta tgggtgttct aattatccaa actgtgactt tgtagcttgg
     5341 aataagccaa caggagagtt ttgtgagaag tgtggttcat atatggttga aaaagtgact
     5401 aaatctgaaa caaaaacagt gtgttctaat aaagaatgta ataaagagac aaaagagcat
     5461 aatgacggaa aagagtgaaa attgaaaaaa atcacataat tctcttgaaa aaggttccaa
     5521 tctatgataa tctttcaagt gtaaacaact aatagaaaaa taaattttca ttaaatgtga
     5581 aaataatatt agaaaatata aaaggttatt aaaaggccta aatttcatga ggagatgatt
     5641 aaatggcaag tgaagtgtta caaaaaacaa ggaaaataaa taaaacatta caaacaagtg
     5701 gtggaagcag tgtctctttt gatttactgg ccggagcatt gggcgacgtt ttaagttcta
     5761 atgtttatgt agtaagtgca aaaggtaaag tactaggtct tcatttaaat gatgttcaag
     5821 acagttcagt tatagaagat gagtatacta agcaaaagaa attttcagat gaatatactc
     5881 aaaatgtgtt aaaaattgat gaaacattag aaaatttaaa tggtgagaag atattagaaa
     5941 tctttcctga agaacatgga agattacaaa aatatactac agtagttcca atattaggaa
     6001 gcggtcaaag attaggaaca ttggtacttt caagatattc aaattcattc aatgatgatg
     6061 atttagtaat agctgaatac agtgcaactg ttgttggtct tgaaatatta agagcaatag
     6121 gtgaagaatt ggaagaagaa atgagaaaga aagctgtagt tcaaatggca ataggcactc
     6181 tgtcctactc cgagcttgaa gcagttgaac atatttttgc tgaattggat ggaaaagaag
     6241 gtctacttgt agcaagtaag atagctgata gagttggtat aactaggtct gtaatagtaa
     6301 atgcacttag aaaatttgag agtgcaggtg tgatagaatc aagatcatta ggtatgaaag
     6361 gtactcatat aagaatactt aatgacaaac ttacagatga attaaaaaaa ttaaaaaaca
     6421 atcaataatc tatattttat aggtttagat tagaaaaggt atctatttca aatagatacc
     6481 ttttttagac gaattattaa ggtaatgata agtatattaa aaaggagtga gaaaatgcct
     6541 ttagcagata aaataagacc aaaaacaatg aatgatgtaa taggtcaaag tcatataata
     6601 ggtaatggaa aaatattgtc taagatatta caatctaatt ttttaccaaa tatgatattt
     6661 tttggtcctc ctggtgttgg taaaactaca gtagcggaaa taatagcaga aagaagcaat
     6721 aaaagcttct ataaaataaa tgctactaat tcatctttag aagatataaa aaaggtgata
     6781 tcagagcttg gaagtataaa taatgtaaat ggagtcctat tatatataga tgaaatacaa
     6841 agttttaata aaaaacagca acaatcaata ttagaattta tggaaaatgg gagtataact
     6901 ttgattgcta gcactacaga aaatccatat cattatgtat ataaagctct tttaagtagg
     6961 tctactgtat ttgaatttaa acctttagaa aaacttgatg tagaaaaagg actgaaaaga
     7021 gctgttgaag tattaaatga agatagctat atggatattg agtgcaataa tgatgctatt
     7081 gaagatatag caattctttc tgatggagat atgagaagag ccttaaatat tttggaagtg
     7141 gtagtctatt caactaaacc aaataaggat aatataactt atatagattc agatgtaata
     7201 agggcatcta catttaataa aattataaac tatgacaaaa atggtgatag tcattatgat
     7261 attttaagtg catttcaaaa atctataaga ggaagtgacc cacaggcatc tatacactat
     7321 ctagcaagac ttataaaagg tggggatttg ataagtatat gtaggagact tttagtaata
     7381 gctagtgaag atataggttt agcttatcca aatgctattg taattgtaaa ggcatgtgta
     7441 gactcagcta tgcaacttgg atttccagaa gcgaaaatac cattagcaga agctactatt
     7501 cttttggcta catctccaaa gtctaattct gcatgtatag caataatgaa agctatggat
     7561 gaactggata aagagtttgt taaggatata ccaaatagta taaaggatgc tcactattct
     7621 ggttctaaag aaataggtag gggttgtgga tacaaatatc ctcataattt taaaaatcat
     7681 tatgtaaagc aacagtattt accagatagc ataaaagata gtatttatta cataccacaa
     7741 gaaaataaaa ctgagaaaaa tataaaaaaa tatttagaat atctaaatag tgatttttag
     7801 actgaacttt atgtatcggg ggtagatatt atgattataa aacttaactc aacttatcac
     7861 agtagagtta tgaaatactt aaaaaaagaa cctgagtaca atttgtttat aataggggat
     7921 attgaaagat atggatatgg aaataatttt ttaaatatat gggcagatgt gggtgaacat
     7981 ggtgaaataa aggcaatatt acttaaatat tttgaattta tgatgtttta ttctgatgga
     8041 gagtatgatg ttgaaggttt ttataatcta cttcgtaata ctaattacga agaaatatct
     8101 gggaaaatat gtgctgtaga tgctttagca aagagattag gacttaataa tttaaaggta
     8161 gttgattttt gcaaacttca aactaaaaag tttttgatag ataataattg taatgcaaag
     8221 gttaagagaa ttaggcttgg aaatctaaaa aagacagtga aactttatga cttaatagat
     8281 gaatttcaca gtacaacttt agagaattta aaaaatggac taaggacagg tagaggatat
     8341 tgtatagaga ttaacaaaca ggtggtgtca atggcaaaga gtacttcaga aaataggact
     8401 catgcaatga taattggagt aggaacacat cctaaatata gagctaaagg acttgctaca
     8461 aaatgcttaa ttaaactatg ttcagagtta ctgagagaaa acaaaattcc ttgtttgttt
     8521 tatgataatg aagaggctgg aaagatatat aaaaaactgg gatttgaaaa cattggaaaa
     8581 tggggaattt attcaaaata aaaaattaaa attaatcttg acaaaattag tagggtataa
     8641 taaaattatc ttgaaagtaa ttcctactaa aacagtatga attagatttc tatatagagg
     8701 tgataatata tgaagttgtc tactaaaggt agatatggac taaaagcaat gtttgaatta
     8761 gcattaaatc aagacaatgg accagtatca ttaaaattta ttgctaagaa gcaaaaaata
     8821 tcagatcagt acttagaaca aatattttca tctttaaaaa aatcgggtct agtaaaaagt
     8881 gtgagaggtg ctcaaggagg ttatttgctt tcaaagaatg ctgaagacat aacagtagga
     8941 gatatacttg tagtattaga aggaccagtt gcattatctg actgtgtact agatgaagat
     9001 gtatgtgaaa actcaaacat gtgtgtaaca aaaatagttt gggaaaagat gaaaaagggt
     9061 atagaagatg ttatagattc aattactctt aaagatatga taaatgacta taacaaaaat
     9121 aaattagaaa atgatataac aaatattaaa aaatagattt ggagatgaat gtaatggaaa
     9181 aaagaagatt gtatatggat tattctgcaa caacacctat taaaaaagaa gtattggatg
     9241 caatgatgcc atacctaaca gattattttg gtaatgcatc tagttttcac acattcggaa
     9301 gagaagcaaa agatgctttg gataaagcaa gagaacaagt agcagccctt ataaatgctg
     9361 aacctagtga aatatatttt acagcaggtg gttcagaaag tgataactgg acactagagg
     9421 gtgtagctta tgctaataaa aacaaaggta atcatataat aacttcaaaa atagagcacc
     9481 atgcaatact tcatacatgt gaatacttag caaaacatca tggatttgaa ataacttatt
     9541 tagatgtcga tagcgaagga aaagtagact taaaacaact agaagagtca ataaaagata
     9601 ctactatact tataagtata atgtttgcaa ataatgaaat aggaactata cagcctatta
     9661 aagaaatatc cgaaatagct aaaaaacata agatattatt ccacactgat gcagtacaag
     9721 ctacaggtaa tattccagta gatgttaaag agttaggaat agatttgatg agtatgtctt
     9781 cacataaaat atatggacca aaaggtgtag gagctttata cataagaaaa ggtgtaagac
     9841 ttcataactt tgtccatggt ggagcacaag aaaaaagcaa aagagctggt acagaaaata
     9901 tacctgcaat agttggatat ggaaaagcag cagaattagc taaggaaaat atgcaaaatc
     9961 atgtagaaac tttaactcgt cttagaaata agttaataga tggtgtgtta gaaagaatac
    10021 cttacacaag agtaaatggt agcttagaaa atagattgcc aggaaatgca aactttgcat
    10081 tccaatttat agaaggtgaa ggaatacttt tattattaga tatgttagga attgctggtt
    10141 caagtggttc tgcatgtact tctggttcat tagacccttc acatgtactt cttgcaatag
    10201 gtttaccaca tgaaatagca catggttcat taagacttac agttggagat tttacaacag
    10261 atgatgatat agattatata ctagagaact taccaaaagt aatagaaaga cttagaagca
    10321 tgtcaccact ttatgatgat gcgaagaaac aaggtttagt aaaatagaca taaagataaa
    10381 gcataaaaaa tagacataaa ttgagtagga ggaaaagata atgcaatata gtgataaagt
    10441 tatggaacat tttatgaatc caagaaatat gggagaaatt gataatgcaa gtggtgtagg
    10501 tgaagttgga aaccctacat gtggagatat aatgaagata tttttagaca tagatggaga
    10561 tgtaataaag gatgttaagt ttaaaacatt tggctgtggt tcagctatag caagttcttc
    10621 tatggcaact gaaatgataa aaggaaaaac tataaaagat gcattagaac ttacaaataa
    10681 agctgttgca gaagctttgg atggtttacc accagtaaag atgcattgtt cagttttagc
    10741 tgaacaagct gtaaaagcag ctcttataga ttatgctcaa aagaacaata tccatatacc
    10801 agaattggat ggatatgtta tagatgatgc tcatgaccat gatgttgaag aagaagaata
    10861 aaaataagtg gttgtgttat gagaagttta caatgattaa aaagaagtac taatgatata
    10921 attaggacat aaaaaatagc tgtttttaaa atggctattt tttattgcct aaattaagta
    10981 ttttaacttg atttagaggt taattagtta acaaaataat ccataagatt caaataataa
    11041 agatgattat ttttttagaa aatagaacaa gctatatttt aaataagtac aatacttgct
    11101 ctatatggta taataataag taatgcaaaa gatgaggtga aatttgttta tgaataaaaa
    11161 agtaatgata ggtatgagtg gaggagttga tagctctgta gctgcatatc ttttaaaaca
    11221 acaaggatat gatgtcattg gtgttactat gaagttgtgg caagatgatg atgttgttga
    11281 aattgaaggt ggttgttgtt ctttatctgc tgttgaagat gctagaaggg tagcaaataa
    11341 aataggtata ccattttatg ttttaaattt tagagaagtg tttaaagaaa aagtaataga
    11401 ttattttata gatgaatatt tggaaggaaa aactcctaat ccctgtatag cttgtaataa
    11461 acatataaaa tttgatgatt tttataagaa ggctagacaa ataggttgtg attatgtagc
    11521 tacaggtcac tatgcaaaga tagaaaagga tgaaagtaca ggaagatatt tgctaaaaaa
    11581 atctgttaca gataaaaaag accaaactta tgcactttat aatcttacac aagagcaact
    11641 agagcatact ttattgccaa taggtgacta tgaaaaagat agagtaagag aaattgctaa
    11701 ggaaatgggt atggcagtac acaataagcc tgatagtcaa gaaatttgtt ttgttaaaga
    11761 taatgactat gcaaattatg taaaaaaaca ttctaaaaag cgtattgaag aaggtttttt
    11821 tgtagatact aaaggcaata tacttggaaa acataagggt atattatatt atactatagg
    11881 ccaaagaaaa gggcttggaa ttacttttgg aaaaccaatg tttgtaatag acataaatcc
    11941 aataaataat accatagttc ttggagacaa tgaagattta tttaaaaagg aacttattgc
    12001 taaagatgtt aactttatat caatagatac gttagaagaa cccttaagag ttcaagccaa
    12061 aataagatat tcagcaaaac catcaccagc tactattcat agagtaggag aagatactat
    12121 taaaatagtt tttgatgaag ctcaaagagc tattactaaa ggtcaatctg tagtaatgta
    12181 tgatggagat attgtagtag gtggaggtat aatcgagaaa agtttataaa aacatctaaa
    12241 aaattaaaaa aataagcaat aaagtatatt aagagtaaat aaagtttata aaataaattt
    12301 attgtatatg aaaatatagt attgattaat tttgtaaaat ttagtaatat aattagtata
    12361 ttaattaaaa aacaaataca gtgataagga atagtaaaat atttgatgtc ttacagagag
    12421 agggaaatgc tggaagccct tagaatatga tatttgaagc agccttttag tagcaatctt
    12481 gaacaattta tcttttataa agaagattta agtagagatt gacggtagtt acgttatagc
    12541 taaggttttc cgaagatatc atactttgta tgataattag ggtggtaccg cgattgtcct
    12601 cgcccctata aatttattta tagggacgag gttttttatt taaacgaaaa aataaaattt
    12661 attaggagga acatgatatg gaaaagatgg gattaaatga aataagaggt aagtttttag
    12721 aattttttga gtcaaaaggt cattatgtag caaatagtta ttctttagta ccaaacaatg
    12781 ataagagttt gttacttata aactcaggta tggcaccttt gaaaaactac ttttcaggtg
    12841 tagaagtacc tccaagtgtg agaatgtgta catctcaaaa atgtataaga acaggagata
    12901 tagaaaatgt aggtataact gcaagacatg ctacattctt tgaaatgatg ggtaatttct
    12961 catttggaga ttactttaaa agagagtcaa tcaaatgggg atgggaattt gttacagaat
    13021 ggctaaatat accagaagat aagatatggg tgactgtata tgaagaagat gatgattctt
    13081 atgatatatg ggcaaaagaa atgaatttcc cagaagaaag aatggttaga cttggaaaag
    13141 atgataattt ctgggaaata ggtacaggtc cttgtggtcc ttgttcagaa atatattttg
    13201 atagaggaga agaatatgga tgtgacaatc cagactgtaa accaggctgt gattgtgata
    13261 ggtatttaga attttggaac catgtattta ctcaatttga tagagatgaa gaaggaaatt
    13321 atagtctatt agaaaacaag aatatagata caggtatggg tcttgaaaga atgggatgta
    13381 ttatgcaagg cgtagacact atctttgaag ttgatacaat taaatctata ctagaagcag
    13441 ttgagaagtt aacaggagta aaatatggag agaatccaaa aaatgatata tcaataagaa
    13501 taataactga ccacataaga gctgtaacat tcttagttag tgatggagtg cttccatcaa
    13561 atgaaggaag aggatatgtt cttagaagac tccttagacg tgctgctcgt catggtaagt
    13621 tattaggagt aaaagaatta tttttacaga aactaataga tgaagttata aaagtaaatg
    13681 ataaagcata tccagtttta gttgaaaaag aaagttatat taaaaaagta gttggaatag
    13741 aagaagaaaa attcaatgaa actatagacc aaggaacaga aatattgaac tcttatatag
    13801 aagtattaaa aaatgaagga aagactgttc taagtggtca agaagctttt aaactatatg
    13861 atacttatgg cttccctata gacttaacta aagaaatatt agaagaagaa catctatctg
    13921 tagatgaaga agcttttaat gaagaaatgg aaaagcaaaa agaaagagct agaaatgcta
    13981 gaggaaatat ggatggagaa agttggaagg aagaccctct atcaaaatta gaatctacag
    14041 ttgacagtat atttaatggg tatagtgaaa tttatggtga aggaactatt gaagctatag
    14101 ttaaagatga tgagctagtt caaagtgcag aagaaggcga taaggtatct atagtactag
    14161 ataacactac tttctatcct gaaggtggag gacaagttgg agattgtgga ttgataacta
    14221 atgaaaactt agttttagaa gtattaaata ctaaaaaagg tgctaataat agtataaaac
    14281 atataggtat aataaaatca ggaagaataa gtaatgggga caaagtaaag acactcgtag
    14341 atagagaaac tagaatgtca gctgcaagaa atcacagtgc tacacattta cttcataagg
    14401 cattaagaga agtattagga gaacatgtta atcaagctgg ttctttagta actccagaaa
    14461 gacttagatt tgatattact cactttgaag caatatcaaa tgaagaatta aaagtaatag
    14521 aagaaaaagt aaataatgtt attttatcat ctttagacat aaaatgtgat attatgaata
    14581 taaaggaagc taaagaaaaa ggagctacag cactatttgg tgaaaaatat ggtgatgagg
    14641 taagagtagt ttctatggga gattattcta cagaactttg tggaggaact cacttgacaa
    14701 atacttctca agtaggaatg ttcaaaatat tatctgaagg tggagtagca gcaggtgtaa
    14761 gaagaataga agcaataaca ggaaaagctg tttatgaata cctaaaagaa agagatggaa
    14821 ttatctctga agtttgtgta aacttaaaat ctaaagaaga taatttaatt caaagaataa
    14881 gttctttatt agaagaaaat aaaaacttat ctaaagaatt gcatgatatg aaagctaaga
    14941 tgagtttaca atcagttgat tctatttttg attctaaagt tgaagtaaat ggagttaatt
    15001 tgataactaa taaatttgaa ggtatggata tggatacact aagagaaaca gcagataatc
    15061 taagagataa gcttggttct ggtgtagtag tacttgcaaa tgtagtagat gataaagtta
    15121 actttgtagt tactgccact aaagatgtat tagataaggg aatacattca ggtaatattg
    15181 ttagagaagt tgcaaaaata gctggaggaa aaggtggagg aagacctaat atggcacaag
    15241 caggagctag tgatgtatct aaggttgacc aagctttaag ctatgcaagt gaggttatta
    15301 agacacaagt taaataacat tcactcaagg aggtttttag tttggaaaaa gatttagact
    15361 acactatgaa atttgaagga ataccagaag ataggatgag tgtaggagat actattgatt
    15421 ttgtatataa agcattagtt gaaaaaggat ataaccctat aaatcaaatt atagggtatc
    15481 tcctttctgg tgattctagc tatataacta gtcataaaaa tgcaagagct ataattaaaa
    15541 aatttgaaag agatgagata cttgaagaag taatcacgca ctatctgaat agaaagtagg
    15601 tcaaataaat gagaaaaaag attatgtcgt tgtttgcaat agtcattatg atttttgtat
    15661 caactgctag ctatggtttt gctgaagaaa aaggcaaggt catatttatt gatatgaata
    15721 ggaccaatct tggaagtatg atgaatattc caatcttgaa aaatgagata gaaaagagag
    15781 gctatgtagc tctcatgaat ataagagggg atcaaggtac tgatgataga aggtcttatg
    15841 caagtatggg tgcaggaaca agagctagtg taactactga agactatatc aattttcaaa
    15901 aagtagacca agatactgct aaagcttttg aagtcgcaac aggtcaaaaa gctaaagaaa
    15961 taaatgattt aggcattaat agatctataa atgaaaatct tgagtatgga gaatatggag
    16021 caaatttagg gtcattagga aaaactttat cagaaaatgg tttaaagaca tctgtaattg
    16081 gaaatgctga tataattgaa aataatcaat tagttaaaaa cagaaactta tgtttagtag
    16141 ctatggacca atatggaagg attgattcag gtaatattga taatattaat aagaaagatt
    16201 ctaaaatgcc atttggaata tctacagatt atgataaatt actagatgaa actaaaaaat
    16261 cctataaaaa tagtgatgct atatttgtag aacttggaga tacatataga ttagatatgt
    16321 atagattaaa tctaaatgat aatacttatg atacaatgaa gaaaaatatt gtaaaaaata
    16381 taaataaata tcttgaacaa gtattttcta tggttggaga aaatgacact gtttatatag
    16441 caagtgcatt tccaagtgac ctagattata agaataagag aagactttct ccaataataa
    16501 aatttagtgg agaagggaaa ggtctactgg aatctgccac aacaagaaag gctggaattg
    16561 ttgccaattt agatgttgga gtagatatac taggtacatt tggtcttgaa aataaagaga
    16621 tggtaggtag aagttatact ttagtaaata aagaggacaa tattagctat ctaaaatcac
    16681 tttatgaaag gatagtgtct atatcttcaa taaggtcaac tattataaat ggctttgtag
    16741 gcgtaatatc tgcttcttgg gtaattgcca tgtttgcgat tttctttaga aattatatgc
    16801 cacataaaga gcgtgtattc aaagtactta aggagtttat aaaattaggc ataataatgc
    16861 cactatcatt tatgttagct cctttgatga attttaaaac tccaatttca atatctttgg
    16921 gaatatttgc gactacgcta atattatact tgttaggtag aaaattattt aaggacaatg
    16981 atataaagca catgggattt tttgcaacac ttactatttt agtcatagtt atagattctg
    17041 tatttggaac atacttaatg caaaataaca taatgagtta tgatgctgtc ataggagcta
    17101 ggtactatgg tgtaggaaat gaatatgaag gtgtaactat agcaagtgca gtatttggct
    17161 tagcagtatt attacattat aagaaggtat ctaaattact tgttgtaatt ttttcattaa
    17221 taatacttat aactagtgca tatccttcaa tgggtgcaaa tgttggtgga gctatatcag
    17281 aatgtgtagc atatttgttg tttataatgc ttatatatga cattaaaata gattttaaga
    17341 aagctgtatt attagctgtt tcagctgtag ttgtcgtttt tgcatttgca gctttagata
    17401 tactttcagg tagtgaatct catttaggta tgtttgtaca gagaattttc ctaaatggac
    17461 caggagagat attacaaaca tttggaagaa aaattcaaat gaatatgcaa ctagcacaga
    17521 caagtgtatg ggtaaatatt ttacttgtag gaattggagt aattgcagtg cttatattta
    17581 aaccatctag acactttaga aagataatga ataattttcc aatcttgttt aaagggttta
    17641 ttgcatctat ggttggttgt ataattactc ttttagttaa tgattctgga atagttgctg
    17701 catctacagc atctatatat atacttatac cactaataat aataagtata aatatgataa
    17761 tatttgaaaa aaataactaa aaaaattttt actgttcata taggatttaa ataaaaaatg
    17821 caatatgatt attaatgtaa actggatgct tacataaaaa atcatattgc aatttttatg
    17881 attgattatg taaaattata tgaagcagta agcctgtgac cttagttatg agaggttcag
    17941 aatatgtatt taatctatag aatataaata gataaaggag gaactatgtt agatggaaga
    18001 ataatgggtc ttgatgttgg tgataaaact attggagttg ctgtgagtga tttgatggga
    18061 ttaactgcac aaggtgtaaa aacaataaaa agagttggca agaaaaaaga tattgaagag
    18121 ctaaaagcaa taataaaaga aaaacaagta aataaaattg tatcaggatt accaaaaaat
    18181 atgaatggaa ctttaggtcc acaaggagaa aaagtaatca agttttgtga attggtaaaa
    18241 acagaaacag gtattgatgt agagttttgg gatgagaggc tttctacagt agcggcagag
    18301 aggtctttat tagaagcaga tgttagtaga caaaaaagaa aaaaggttat agatatgttg
    18361 gcagcagtaa taattttgca aggatattta gattttaaaa taaattcata aaagaactat
    18421 aaaattgaat acttatgata tataatataa ataataaaaa taaaaataaa tttgaggtga
    18481 atgacattat ggaagaaaat ataataaatt taatagacga aaatggcgtt gaaagccaat
    18541 ttgaaataat attaacatta gaagcagaag gaaaagagta tgcaatatta atgcctttag
    18601 atgatgaaga agctgaagaa gctttaatct ttagaattga tgaggatgaa gaaggtgaaa
    18661 tcttaatacc tttggaaagt gatgaagagt atgaaactgt tgtagctgtg tatactgcta
    18721 taatggaaga agaaggttta aattacgacg aagatgaatc aaatggactt aactagataa
    18781 gtaattaagt ttgtatataa aaatcagata tttattatat ttgctttaat taaaccttat
    18841 taattgtaaa agcatttata gtatatatct gtttttttgt tttttattaa aaataccaaa
    18901 aaatttatga attttggtga ataatatatc ttttatgggt atatatttat attatgagaa
    18961 taaattaaat ttatttaaat gtcttgacaa aaaatgaatt atcaattaca atattattta
    19021 ataaagatta tcaattgaaa atagtaaggg tgatgttatg gcaaatacaa tggatttatt
    19081 aaaagacaag ttaaaagaaa ctggttttaa gattacgcca caaagaagag caatagttga
    19141 aatattgctt aaacatgacc attcacatct aagtagtgag gaaatctatg atttagttag
    19201 ggttgactgt cctgaaatag gcttagctac tgtatataga acaatgcagc tattggatga
    19261 aataggatta atatcaaaac taaacttaga cgatggatgt ataagatatg agattagtct
    19321 acataaagaa gactgtcata atcatcatca tcttatttgt aaaaattgtg gaaagataat
    19381 ggaagctaaa gaagaccttt tagacaatat agaaaaagag atacaatcat tgtacaaatt
    19441 taaaatactt gatcatgatg ttaaatttta tggactatgt gacgagtgta atggagtaag
    19501 cgattcagaa gaataaactt ctattgctta atgtcgatgg aagaatagta tagaactttt
    19561 atattctatg ctattcttcc attttttatt ataaaccatc tacaaaatgc aaatatacag
    19621 ttttcaagat gcttatagtt ttaatattga aaatataatt aatcctataa gtacaaaaat
    19681 gaatgatttt tctatgaaat tactaaatgg tcctccaaca gttaaaaaac ctaaagaaat
    19741 tttcttttcg cttagcggat aaaaaattgg tattccttgc tttgtaaata aatctcctaa
    19801 aaacatatga cttgcataac caatcgtacc ataaaaagat aaattattaa tattagtgat
    19861 taattctatc tgttttagta aaaaatatac tattatagtt gcaaatatgc tatgagaaaa
    19921 acttctgtgc tttaaccagg gaaaaattga caacattaga caaaaaattg taaaatatat
    19981 ttcaacttct atgatgtata agcatatagc taatagtata aatattaatg atagaaaaac
    20041 ttttcttaga aatgtgtggg ttagttttgc ttcaataatt ataattgcaa caaatgtaat
    20101 tattgcgctt aaataaaaat tatcatttat atttattgaa ataaagaaaa taataatatt
    20161 aacagcatat ataaaaattc tatatatgta tttggataca ttgtttttta gaatgaaatt
    20221 tgagaccata gaattagatt tatccaaatc gggtagtaca gcaaaagtag ctgaaactaa
    20281 aatatcaact aaagatatag gaattttaaa tattaaagat gtttggatag ctgttaagat
    20341 tcctattgca caatgagttt taccacgcat aaatcctcct aaattatccg atattaaatc
    20401 atataatatt tagaggatat caaaataaaa taggtttgtc aaaataatac gttaaatgta
    20461 ttttaaatat agcttaaaat atgatacgaa ttatggtaca ataagaataa ctggtagcga
    20521 gtgcgtgatt tgatattatt tatgattgta gaacgtgtta aaaaattaaa tagacagaag
    20581 gagatgatgc aattgttcaa aaagaacacc aacaagataa aggtaatggc cttgggtgga
    20641 cttaacgaag ttggaaaaaa tatgactgtt gttgagtata aagatgaaat aattgttata
    20701 gatgctggac ttagttttcc agaggatgaa atgttaggag tagatatagt tataccagat
    20761 ataacttatt tagtaaaaaa tagggataaa ataaagggta tatttattac acatggacat
    20821 gaagaccata taggagctct tccatatata ttaaagaaaa taaatgtacc tgtatatggg
    20881 gcaagactta gtataggtct tatacaagta aaacttaaag aacataaaat gaataatgtt
    20941 aagttaaatg ttataggtcc tagacaagtt ataaagcttg ataatatgga agtagagttt
    21001 ttgaaaaata accatagtat accagatgct tactctattg caatacacac agaccaaggt
    21061 ataatatacc acacaggaga ttttaagatt gatttgactc ctattgatgg agatgttatg
    21121 gatatgcata gaatctgtga gttgagcaaa aaaggagttc ttttaatgct ggcagatagt
    21181 actaatgcag aaaaaccagg atttactatg tctgaaaaga ctgttggggt aggtcttgac
    21241 gaattatttg caaaaggaaa tggcagaaga attatagttg ctacctttgc atccaacata
    21301 cacagattac aacaaataat aaatactgct gaaaagttta atagaaaagt tgctatatca
    21361 ggtcgctcaa tggtcaatgt agttggagtt gcaaaagaat taggatactt agatatttct
    21421 gatgacatgc ttatagattt aaatgatata tgtaagtatg aagatagtga attagtgata
    21481 ataacaactg gttcacaagg tgaacctatg tcagctcttg ctagaatggc tttttcagag
    21541 cacaaaaaag tagaaataaa gagtggcgat ttagttataa tatctgctca tccaatacca
    21601 ggaaatgaaa aattaatatc aagagtaatc aatttcttat ttgaaaaagg agcagaagta
    21661 gtatacagtg atatagctga tatacacgtt tctggacatg cctgtcaaga agaattaaaa
    21721 cttatacatg cattagttag accaaagttt tttatgccag ctcatggtga atatagaatg
    21781 ttaaaaagac atgctgaaat agctgaacag cttggtatgg ataaagaaaa tatttttgtc
    21841 atgcaaacag gagatgtttt agaattagat aaaaactctg ctaaggtagc aaaccgtata
    21901 caaacaggaa acatattagt tgatggtcta ggagtaggcg atgtaggtaa tatagtttta
    21961 agggatagaa agcatttatc agaagatggt cttatgatag ttgtagttac tatttccaag
    22021 gatgagggta aggtcttagc aggtccagat ataatttcca gaggattcgt atatgtaaga
    22081 gagtcagaag atttaatgga tggagcaaag gatataatta agaatgttct aaatgaatgt
    22141 gaagagaaaa atattaaaga atgggcttat ttaaagaata atattaaaga aaacctaaaa
    22201 gaatatttat atcaaaaaac taaacgaaat ccaatgatac ttcctatcat aatggaagta
    22261 taatataaaa aaactataaa tttgaaaatt agacctcttg taatttttaa agtaatatca
    22321 aaaatccaat tgatattatc ttaagaatta caagaggtct ttttaatact cattttaaaa
    22381 aaatatatat tacataaaat accaattata acaatctttt attagtaaaa actacttata
    22441 tataaatttt actgatagga ggattcttat ggatgatgtt tcaagacaaa atgctataaa
    22501 agctttaaaa caaacaaaaa tggaaatagc aggagagtat ggaatgaatt atgaagatgc
    22561 ttttgaaata atagaaaatg cgtctaataa aggtgtttta gaaggttact ttaaaaagct
    22621 tgaaaagaaa aaaaatttag gtcaaggtat atctaggcat ttagaataaa aaaagttaaa
    22681 tgtgagaaaa taagtttaaa taaactttaa gaggtgacat cgatgaaaaa attagctagt
    22741 actgctttgg ctatattaat tgcgcttact cctttaagct tttcttttgc aaataataag
    22801 gaaaatgctg atgccaacca actaaatata tcttcaaaat cagcaatttt gatggatgtt
    22861 ggtagtggac aaattttata tgaaaaggat gctcataaaa agcttcctcc tgctagtgta
    22921 actaaggtaa tgactatgct acttatagta gaagctttgg attcaggcaa gattaagctt
    22981 gatgatgaag ttcaagtaag tgaaactgca tctagtatgg gtggtagtca aatattccta
    23041 gagccaggag aaactcaaaa ggttgataca ttattaaaag gtatagctgt tgcttctgca
    23101 aatgatgctt gtgttgctat ggctgaacac ttagctggaa gtgttgaagg atttgttgat
    23161 agaatgaatg ctaaagcaaa agagttaaat atgaatgata ctcattttgc aaatacaaat
    23221 ggacttccag ttgcaaacca ctatacatct gctcatgata tagctttaat gtctagagaa
    23281 ctattaaaac acgaaatgat aagtaaatac ttaactacat ggatggataa ggttgtagta
    23341 gggaagaaac aagttacagt aggtcttgca aatactaata aacttataaa gcattatcaa
    23401 ggagcaactg gtgttaaaac tggttttact caagaagcaa aatactgttt atcagcatct
    23461 gcaaagagag gtaatactca tctagttgct gcaactttag gagcagagac ttctccagaa
    23521 agatttaatg atgcctctag ccttttaaac tatggatttg ctaattatga aagtgttaag
    23581 ttatgtagta aaggtgataa tatagcaaca cttacattgg ataaggctga tgaaaacaaa
    23641 gttaaattgg ttgctaaaga agatttaaat gctcttatta aaaaaggaag ttctaaagaa
    23701 tttgagaaga aaattgaaat agtaaaaaat cctaagatgc caattaaaaa gggaactgtt
    23761 ttaggaaaaa taaagatatg taaagataaa aaagtaatag gcgaagtgga attgataaat
    23821 actaaggata taaataaggc aagctatcta caaatgcttc aaagaataat tgataatatg
    23881 atataaataa ttatcagatg aaatataatt ataatacaca aagatatgat tataatacac
    23941 ataaaaaata cccttatgta attatttgcc ataggggtat ttttatttac catatagaag
    24001 tattttataa tcttatgatt tttatagaaa tatagaaaag tttatgtatc ttaaaatatc
    24061 atttgattta atatcatata tagggttgtt taacaattat ctaagtaaag gcatactacc
    24121 agtaagttta ttcactttct ttttattccc ataaattgct atcccaaaat actgtaactc
    24181 atcttctgga acactagcaa tcttttgtgt aaattcttca taaagattgc aactttgtgc
    24241 tacatctgaa aaatctacaa cagttaaatc gttaaattcc aaagtatata attttttacg
    24301 taaatctttt attatttctt tgtttccttt taaaataggt acaggaatgt caataattcc
    24361 taagtggcta ttacctgttt tgtcaattac atctgggcca acagtttctg gggcatgttt
    24421 tcctagtgta attcccatta tagctgctgt attggatata attcccattg gtaaattttc
    24481 gtctattacc atgacacatt ttgaattttt attttccatt tttatttctc ctttttacct
    24541 tatttaaagt tgtttgaatc atcatttata aatatatttt gatactgctt tggtgtaaga
    24601 ccaataaaat tcttaaaaaa gtttgtaaag tgactttggt ctacaaatcc tgtttgtatc
    24661 gcagcatcta taggttctac gcctttttct aaaagctttt tagctttgtt tacccgtata
    24721 ttttctaggt atctatatgg tgtaatacct tttaatttcg taaaattacg aagtagagag
    24781 tacttattca tacctgctat agtgcttaat tcgtcaagta ctatatgttc agcataatta
    24841 ttttctagat aatcacatac tgcttgaatt tctatatttg tattttccaa gttagattgc
    24901 aaatttggct ctgtatgttc ttcaattaat tgttcaatta ggaagaaaaa aagctcttcc
    24961 tttttaaaat ccaattcttc ttccatgatt atatagtgta attcttgtag tagaggtact
    25021 agctcactgc gaaatataac aggttgatta aattttggta aataattttt acctgtaatt
    25081 tcaaagacgg tttttttcat tatttctggc ttaatattaa tacatctgta atcaagtact
    25141 ttatcatcta tttgttcaca agtatgacta tcaaaagggt taaaaagtag caaatctcca
    25201 gtacttgtgg tatactcttt atttttacaa gataaatatc tttgaccttt ttctataaaa
    25261 ccaattacat aatgttcatg aaaatggttt gggaattttt gcattatgcc tttaaatcta
    25321 tatgcttcaa tttttaattc cctatcaaag catactgtgc gttcttcttg tctcacttaa
    25381 gtaacctcct ttcatgttat acaaagtgta acacatattt ttttatactt cttgtataat
    25441 attgactaag agacaataat gtcaattcat gtcaatataa tattatactt acagtaggtg
    25501 taattattat attttcacaa tgaatatgat ataataataa aagttataaa ataaaaagca
    25561 aattttaatt ttatgcctag aagttaaaat tattataatg taaaataatt ttgattataa
    25621 gctatttaat tgttataata atattaaaaa tttaatttag gagatgtaca ttaatgttaa
    25681 gtatgaatat aggagaaata gtagcaagtt tagttggtat agctatggca atatcaatac
    25741 atgagtttgg ccatgcttat tcagctcact tgttgggaga tgatacagct aaagcttatg
    25801 gtagaatgac attaaatcct gtaagacatg ttgacccaat aggtcttttg gctatgttta
    25861 tccttaagat agggtgggca aagccagtac cagtaaatcc aaataacttt aaaaactata
    25921 agataggaaa cctgatagtt tcacttgcag gcgttttttg taatgttttg actgctatac
    25981 tgtgtgtaat tatcaataag tatgtgcata tgtatgctat aaatacaata gctggatatg
    26041 tgcttttata tagtatggga tttgcagctt ttaacttact tccgatacct ccacttgatg
    26101 gatggggagt tatatctaca tttgtaccat ataaatggaa tgaatatttg tataaatttg
    26161 aaagtatgag ttatataatt ttattaattg ctttatttac tggtatatat caaataatac
    26221 tatcaccaat atatacagtg ttgctaaaaa tagtatattt atttgtttaa tataataaac
    26281 agattaaaga gggattgata tgaagtataa tatacaatta caagtctatg agggaccatt
    26341 agatttactt tatgatttaa taacaaagca taaaatagac ataaaggata tatctataat
    26401 agatataact aagcagtatc taaattattt gaaaatgcta gataaaatgg atttagagat
    26461 aactagtgaa tttataacta tggcatcaaa acttttagag ataaagtcta aatatctgct
    26521 ttataagcaa aaagatgaag aagaagaccc aagaatagaa cttatggaaa agttagaaga
    26581 atatagaaag tttaaggtag catcacaaga tataaaagaa aatataactt atgttaatga
    26641 gagattttat agaaataaag aagagataat tattgatgac aatgtggatt tagaagatat
    26701 atcaatagaa gcaataaaaa atatacttcc atacatattt aaagttaaaa catcacagat
    26761 agaaaatgct aatgatgaga aactagataa aatagtaagg aagaaaataa tatctgtaga
    26821 agaaaaaata ttatatataa gagatattat aaaagataag attgaagtta cttttacaaa
    26881 tattataaaa tcatatgaaa atgatgaaat aatagctact tttttatcta tattagagct
    26941 tataaaagaa aaagaaatag tcgtagtaca agatatattt tttgatgata ttctaataag
    27001 aaaaagttcg gagtgttaaa atgaaaagag aagatataaa gtatattata gaatctgtta
    27061 tgtttgcata tggtgagcca ataagtataa aagaattaaa ctatataata aataaagaac
    27121 tttcatcaaa agaaatagag attatgttaa atttacttat tgaggagtac agagaacaaa
    27181 atagaggtat tcaaataatc aagttagaga ataagtacca aatgtgtaca aataaagact
    27241 atgcagaata tataaagaag attattgaac caaagaaaaa gaaaagtttg agccaagcta
    27301 cattagaaac tcttactata attgcctaca aacaaccaat aacaaaggta gaaatagagg
    27361 atataagagg agtaaaatgt gataaggtat tacaaacttt gtttgaaaat gaacttataa
    27421 gagaagctgg tagattgaat aagataggga aaccaataat ttataaaaca acagatgaat
    27481 ttttaaagtt gttgaatata gaaagtcttg aagaattgcc acctatagaa aattatcaag
    27541 aagtggcaac aaatgaataa aaatataaaa tcacaataat ttagtatgta attttaaatt
    27601 ttaaaagatt taaaatgctc taagttgttg tgtttttttg tataaatata aaattataat
    27661 tctcttaaaa taaatatgga taattaagta taaatagtaa ttttttgatg ataatataaa
    27721 catggagctt aatgaattat ttaattttaa gtatttaata atagctgtaa tttttattat
    27781 aagtgtaact atgttgattt taatatctca tataaacatc ttaataatag ctgatataaa
    27841 aaacaaagat atttgtttaa agcttaatat aaaatacatg tttaatttaa taaatataaa
    27901 tagacaactt tatccagctg aaaatagtaa aaataatgat aaaaaagagg ggatgaagaa
    27961 taatatagat tcaagtatat tattagctga tgaccttttg agcatttata gattattaaa
    28021 aaagataaaa atacatgaac tttattcaaa tattaatttt ggaactggaa atattggctt
    28081 aacatcttct gtatatgtat tgataaatac tttgtatgga aatttattta atatgattga
    28141 tgcagaaaaa atgtatttaa atgtaaatcc agattttaca aaagattatg ttttaggaaa
    28201 tataaggata catataaggc caagaataaa agctttattt aatattataa taatgattaa
    28261 taaaattatg aataaaaata aaggaaataa ggaaggtgat agtaatgaga gcaacaggtt
    28321 cgatacagag tcttatggaa acaactcttg aaacaattaa aggttctata gatgcaaata
    28381 ctattatagg agaccctata aaaacagata ctacagtggt tgtacctata tcaaaggtta
    28441 caattggctt tggtatagga ggaggagaat actcaaaagg atatgatgat aaagataggg
    28501 aaattgaatt aaaaaatgaa aaatcagata cgaattttgc tggaggtagc gcaggggcta
    28561 tatctgtaca accagtagca tttgtagtag ttgaaagtgg agagactaga ataatgagtt
    28621 tagatagtaa tatcaatcta gtggataata tattatctat aactcctaga gtattggaga
    28681 aaattcaaaa tatatcacaa agtaataaga atcaagataa aaacaatatg taagaataaa
    28741 atatttttaa atatgtatat agttttaagt aaagtagtgg ggttaatata aatcccacta
    28801 ttttgttgtg tactttaaac atttatatct aataagattt tagtttaaaa caatttaaat
    28861 agataataaa gttgatatat agtataatta taaaaagaaa agaagtatta tattatagat
    28921 atataaacgc atttgagata taagagggag ttataaatca ttagttttga ggtgaatgtt
    28981 atgagcgata aagaattatc tattttgttg ttagaaagta tgaataattt tcataattta
    29041 ataaagatta ttaataacga aagatataaa aaagataagg ttcttacaga aagacagttc
    29101 tttgccttag taaaaataag aaagcatgat aaaatagagc ttaaaaatct aagtagagat
    29161 ttgcatgtat ctacttctag tctgtgtata ctacttaata aattggttga gcaagagtat
    29221 gtatataggg aagaagatag tagagataga cgaaatactt tttatggaat aactaaaaat
    29281 ggagaaaaga tattggataa tgaaatacta aagtttgtat ctataataag tgataaaatg
    29341 gattgcctag atatagataa taaagataaa ttatttactt ctttggaaga gtctaaaaat
    29401 ataatagaac aattatttta aaaaggagaa tttattatga atataaagta ttctaaagag
    29461 atattaaaaa gtttagaaaa tgctataaca gtagtagctc tatttgaggg agaagatgtt
    29521 tctacgacat ttgataaaat aaatatgata agcagaaatt ttaattctaa agaaaatagt
    29581 atagatactg taacattaat tgaaaataac aatttggtaa atgtttgtct tgttggtttt
    29641 ggtaagagag agttattgaa tagagaaaaa attagaatca taggtgggaa tttgtgtaaa
    29701 aaattaaaaa atatattgaa aaatgaagca tataagagta gtgatgttaa tgtactgaat
    29761 ctcaatttaa atagtgatga aataggttgt tttgtagaag ggatattact tggaaattat
    29821 aagtttgata agtataagac aaaaagtaag gattttgaaa gaaaagaagt tgatactata
    29881 actatattta ctgaaaaaga agttgagtgt caaatagaaa aggctaaaat attagctaat
    29941 tcaactataa tagctagaaa tctagttaat gaaccatcaa acgtaatata tccaaaaaca
    30001 ttagcagtag aaacagttaa acttggtgca gagtttgggt ttaatgttga tgtttatgaa
    30061 gaagaaaaaa taaaggcatt gggaatggat gcttttttcg ctgtttcaag aggttcaatt
    30121 aataaaccaa gattcatagt aatgagatac tttggagata aagaaagtac agatatatta
    30181 ggtctagtag gtaaaggatt gacttatgat actggtggat attctttaaa atcaaatgct
    30241 tctatgctag atatgaagac agatatggct ggtgctgcga gtgttatagg tgctatgtgt
    30301 gctatatctc aaagtaaatt aaagaaaaat gttatagcag tagtggctgc atgtgagaat
    30361 gctttatcag gaggttcata caaacctgga gatataatta gttctatggc aaagaaaact
    30421 attgaagttt taaatacgga tgcagaaggt agacttacat tagctgatgc aatatactac
    30481 attataaaca atgaaaaagt aacaaaggtg gttgatgtag ctacattaac tggtgctgct
    30541 ttaacattgt taggtaatgt tgcaactcct atagtaacaa ataatgatga tttttattgt
    30601 gaattagaaa aagctgccac tttatcaggg gaaagggtat ggaagatgcc aatttatgat
    30661 gaatttaaag atatgataaa aggagaagaa gcagacctta aaaacactgg tggtaaaaat
    30721 gctggatgta taacagcagg agcttttatt ggtgaatttg ttgggaatac tccttggata
    30781 catatggata tagcggggac aagtacatca tcaaaatcaa ttggatacaa agcaaagggg
    30841 gctacaggag aacctgttag aacattgtac tatttggcag aaatctaaaa aacaattata
    30901 aaatataatt tataaagcca cgtggatatt tttgtaattt aagattatct atgtggcttt
    30961 attttttatt taagtaaatt atttactctt tttctttttt tcaagtataa aagtatctct
    31021 attaaataaa agagcaggta tgtacataat actagtaaca aaaataaacg taaaaatagc
    31081 agttgtgcta tgacttaaaa aaccataata gtacctagag ttggtaaatg tgtttagtaa
    31141 aaatatgaat gaaaaataaa taaaacatat agctaagtta tctctaagtg catatctgtt
    31201 aactctaaat tttctaacat caaccatata tatatctgca taaattatca agcacttaga
    31261 taagataaag ataaaatata ttaagctagg aaattcatag cccaagtttt tagaaaaaat
    31321 gagttctcca actgaacaaa ctaaactcca agaaaaaaat atattaaaaa gatgatactg
    31381 cttaaaaatt agtatggcta tacacagata tatacaaata taattgattc caattggtaa
    31441 agagtttaga gtactataat tacctgaaga aataagtaat aattgctcta gagccataat
    31501 tattacaagt aatgtacaaa ttatcttctc aactgtatag ctataaggta aaatattttt
    31561 ggttagtttt ggacaatagt ataaaaataa tccaaatata aagagactta caaattgttc
    31621 actagaaaaa agaaaaattt taaacatata acattctctc ctataataaa tttaatatgt
    31681 atctatataa atatattcat tatatctcaa tttaatatat aaaaaagata ataatagtta
    31741 gattttttat atgaatttta ataggtaaaa tatcctattg ttttgtagat atatcctaat
    31801 tatgtcaaaa aataaaaaaa tttttattta ttagagtttt tggaaataat aataacagat
    31861 attataatta attaacagtt tttgtaagta tttgacaata gagagggtgt aacaaatggg
    31921 aataatagag tcagcatcaa aattggctga gatggttcat ttattggctg ttgaaaaggg
    31981 gataactgat atagaagcat gggatgaagc tgtgaaagaa tacagcaaaa tttatgaaga
    32041 aagaagaaat gaatagaatg ataaaaaatg aaaacttgat gataaaaaat aactttattt
    32101 aaagaaattc aatttttata gaagtattaa aaataatata aataaaaaga tggtatttca
    32161 aatgtaagta aaaaatcatt ttgggatacc atccttatta tttattttaa taatatataa
    32221 gtaataaatc ttacttatcc atatttttta tgtaacctct tttttctaac ttttttatat
    32281 gctccttacg tatttcatca agattaatac catattgttc ttctagctta aacatcatag
    32341 taacacatgt ctgcgctaca tccattagtt ccttagcagt tttctttata atatcaactt
    32401 catctaaatc attattttct ccacttagat ttctgaattt acctatacat tcagctaatt
    32461 caccttgttc ttcacccatt ttgataaatg tagattctag tgttgggtct aaattattaa
    32521 gctctggtaa agatatagtt tttagtttca taaaagctcc tttcaagtta ttcgtattta
    32581 tactaaaaca tcataacaaa aataatacta aaaaaaaact ttaaattcaa cataaaaact
    32641 tacatatata ataaaaatcg tgaaataaat ctatatttgt cttaatacag cctcatataa
    32701 aacttctcca ataggtttat ttattacaag agaggctgac ttatcataga ctgtatttgc
    32761 tttattaata agagcaatat aatcgccttt gtaataatct ataaaacttg ctgctgggta
    32821 tacagctaat gatgtacctc ctataatcaa taaatcagca ttacttatag cagaaattgt
    32881 tttagtaatg acatcactgt caagagcttc ctcataaagt actacatctg gttttactat
    32941 agaaccacag ttgtcacagt aaggaatatt acctcccaga tttaacatag attctaagtc
    33001 aaaaaactta ccacatttag tacaataatt tctatgtaca gaaccatgaa gttctagcac
    33061 atttttactt ccagccattt gatgaagtcc atcaatattt tgagttatga cagctttaag
    33121 cttacccatt tcttcgagtt ttgctaaagc tatatgagca ttattgggtt ttgcattagg
    33181 atatattaat ttatccttat aaaattcaaa aaattcttct ggatatctaa caaaaaaagt
    33241 atgtgaaact aattgttcag cagtaaattg tttattgagt ttttggctaa ataatccagt
    33301 agaacttcta aagtctggaa tattagattc agttgaaaca cctgctccac caaaaaatac
    33361 aatattattg tgattagcta ttaagtcttt taagctattt gcatccatag ttacacctcc
    33421 aaattattat atataaattg tactacatat taggtcgttt atgttttatt aatttaaata
    33481 aatatatagt ttaataagtt aaataaaggg aatataaata aaatgtatac agagtataaa
    33541 tagacaatat ttactaaaat aacaagttgt cgtataatta tattaaaatt gttttagtgt
    33601 ataagaaggg cgggggaagt acgtgaagaa aaaagcagct ttagcaacat tggctatgtt
    33661 acccttaggt gtggtaaatg cacatgctga tggagatata ggtatagtga ctataaatta
    33721 tttaaatgta agaaatgaac caactgccga aagtagcata gcctttgttg ctaaaaaaga
    33781 tgataaagtt ttgattaaag actcttctaa tggatggtat aaaataaaag ctgaatctgg
    33841 acaagaaggt tgggcttcat caaaatatat agcaaaatca aatagtgact ctttaagaac
    33901 gtctacaaat aaagaaaaac aagttatttc aaatagttta aatatgagaa atggagcagg
    33961 tacaagttac agagtaataa ctgtactaaa aaaaggtcaa aaagtagaag taatatcaga
    34021 gagtaatgga tggtctaaaa tcaaatatga tggaagacta ggatatgtat ctagctctta
    34081 tttaggagac gtttcaaact caactaataa atcaaaaact aaacaagtga atacaacttc
    34141 actaaatgta aggagtggac caaatacaag ttatggtttg ttaggcaagt tgccaaaagg
    34201 aagtaaagta gaagtaatat cagaaagtaa tggatggtca aaaataaagt ataatggaaa
    34261 agatgcctat gtatctagta tgtacttatc ggatgtaagt caaagtaatt cagacaattc
    34321 tagtcaaagc aatgataaaa agaatactga taaggtcgta aatacagctt ctttaaatgt
    34381 aagaagtgga ccaggttcta catatagtaa gttgggaaaa gtttataaag gaagtaaagt
    34441 aactgtacta tcagaaagta gtggatgggc taagattaat tttaacaata aagaagcatt
    34501 tgtagtaggc aattatttat ctacttcagc agatacttca aataataact caaatagtaa
    34561 ctctgataac agttctaata gtaatggtaa taattcttca tcatcaggcc aagtaaatgg
    34621 tatgtcaggt ataagtgggg ctaaaattga ttataaatct ttgagttata ctttagaatc
    34681 tcatataagt aaacaagttg agaaagcagc atcaggagga aatgtgatag ctccaagtaa
    34741 taggaaaagc actccaagtc ctgaatttag tactttttca gctcaaagaa caagctcatt
    34801 tgtaaatgca agttcaagtg atatagaata ttatttaaat cctaagaatt ttacaaatac
    34861 tactaaaggt atgatgcagt ttttaaagat taacagttat agagatggta tttcagaatc
    34921 aagtcttaac tcgtatctta atggtttatc ttctagtgta tttaaaaacc agggagcagc
    34981 ctttataaat gctgccaaga agtataatat agatgttgta tatctagtat ctcatgctat
    35041 gtgggaaact gcttatggta agtctacact tgcccaaggt caaacattaa catcttataa
    35101 gggacaacct cttagtaagc cagttaaagt atacaatttt tttggtatag gagcaataga
    35161 taaaagtgct aatgtttcag gtgcagaagc agcgtattcc aatggctgga ctagtgtaga
    35221 agctacaata gatggctctg caaagtggat atcacaaaat tacgtaaata gttctaagta
    35281 caatcagaat actatctaca agatgaagtg gaattatgat tatacatggc atcagtatgc
    35341 aactgatgtg aactgggcta atggaatttc tggaattatg gaaaatttaa ttggtcttta
    35401 tggaggagga agtagtttgg tttttgaagt tcctcaatac aagtaataca taaataaaaa
    35461 atatagacta tgggtagttt tcattttatt taaattatct atagtctttt tgtatataat
    35521 ttataatctg taaatttaat gctaacaaaa cttttatgtt ataatattaa ttctaagtaa
    35581 atataagtga gtaataggta gaaatagata aaaagtggta ataatatata taaagataat
    35641 tttgagaaag gaggatatat atggaaagta ttaaggaata tttggataag ttagaaatta
    35701 ataatagtgg tcttggaaag caacttaaag aagtttatat aaatagagta gtttatttta
    35761 aggaagataa gatagtttat ttttatctaa cttctaaaga tattgtttct catgaacttt
    35821 tagataaatt taaagaagaa ttgatgtata aactcgatta ttttaaagat atgaggatga
    35881 agataagatt tactggtctt gaaagaaaat ccaataaaga tgttataaaa aaatattgga
    35941 ataatatatt gtatatatta aagtatcttt gtccatctat tgcagggtgg tataaacaag
    36001 tagaatttct ttgtttagaa gaagaattaa aaataaaact tccaaaaggg atattttatg
    36061 agcgattaat gaaaaaaaat gtaacttatg tacttaaaac cgtgcttagc gaagagttgg
    36121 gattagattt aaatataaca atagaaaaag ctgttgatga aaaggtaaat aaagaaagac
    36181 tcataagaat caatgataga gaaatggaag aaaaaattag agctttagaa attggaaaag
    36241 ttaataactg tgaagaaaat gaagaggaaa gctatgtcat taagtctgat gtagatgaaa
    36301 atttaatcta tggagataat gcaaatgcta tgattgaaaa tatagttgaa ttaaatgcat
    36361 cttctggaac agttgcagtt gttggagata tatttgatgt tgacactaaa gaacttaaaa
    36421 acggaaaaat acttatgata gcttcaatta cagattatac tagctcaata agctgtaaac
    36481 tttttttaac agatacaaat aaggatggag tacttgaaaa tgttaaaaaa ggagcatact
    36541 taaaaatcaa gggtgacata gtttatgata catatcaaag agaaattagt atgatgatta
    36601 gtgggataag aaaagaaact agaatagaga gagaagataa atcagaggaa aaaagagtag
    36661 aactccatgc acatacacaa atgtcttcta tggatgctat ttgttctgtt aaaaagcttg
    36721 tagagcgtgc agccaaatgg ggtcattctg ctattgctat aacagaccat ggtgttgtac
    36781 aaggatttcc agatgctatg aatgcaggta agtcaaataa tataaaaata ctttatggag
    36841 tagaagggta cttggttgaa gatgattctc tcattataga ggatgcaaat gataaagaac
    36901 tttcgcagac atttgttgta tttgacatag agacaactgg cttttctaat acaaatgata
    36961 aaataactga gattggtgct gtaaaaatag aaaactttaa gattgtagat agatttagtg
    37021 agttaataaa tcctgaaaaa gatatttctt ataagataca ggaattgaca ggaataacta
    37081 atgaattaat taaagacaag ccaaccattg aagaagttct tcctaagttt atggaatttg
    37141 ttggagatag tgtattagta gcacataatg cagagtttga tactggattt atatcgcaaa
    37201 aatgtaggga acaaggcttg ttatataata acaaaaaagt agatacactt atgttagcaa
    37261 gagtaatgtt gccaaactta aaaagacata gattaaatgt cgttgcaaaa gaacttggta
    37321 ttcctctttt aaatcaccat agagcagtgg atgatgctga agctacagca cttatattta
    37381 ataagttttt gcagatgctt acagaaaaag gtgcaaaaac tctaagtgat gtaaacaata
    37441 tacttggaaa gattgattac acaaagctta gcacaaatca tataaccttg atagcaaaaa
    37501 attctatagg tataaaaaat ttatataaaa taatttcaga tgcacatgta aatcattttt
    37561 ttagagcacc aagaatcctt agaagtgtat taaatgaata taaagaagga ttaataattg
    37621 gttcggcttg tgaagctggg gtggtttttc aagctgtaaa gaaaaacgtg tctgatgagg
    37681 aaatgaaaaa aataatagat ttatacgatt atattgaagt aatgcctata gataataata
    37741 gatttatgat tgataaagga gaagtaaagg acgaagaaga gttaagggag ttgaatagga
    37801 aactaattga tacagccaaa aaatttgaca aaattccagt agctactggg gatgttcatt
    37861 ttttagacaa acatgaagct gtcttaagaa aagttcttaa atattctcaa ggttttaaag
    37921 tagatgaaga agaaacttat ttacatttta gaacaacaga tgaaatgttg gaagagttta
    37981 gttatcttgg agaagaattg gcatatgaag ttgtagttga aaatagtaat ctgattgccg
    38041 atatggttga agatataaaa cctataccag atgatactta tcctccaatt atagagggct
    38101 cagatgtgga gttaagagaa atgtgttaca aaaaagccaa acgaatctat ggagacccaa
    38161 taccagaaat agttcaaaaa agactggata gagaattaaa ttcaataata agcaatgggt
    38221 atgcagttat gtatattata gctcaaaaac tggttgcaaa atctctttca gatggatatt
    38281 tagttggttc aagaggctca gttggctcat cttttgcagc tactatgagt gatataacag
    38341 aagttaatcc tcttccagca cattatattt gtgaaaacga agactgtaaa tattcattct
    38401 tttatgaaat aggtgaatgg ggctcaggtg ttgatttacc agacaaggat tgtccaaagt
    38461 gtggaagaaa acttgctaaa aacggtcatg atattccatt tgaggttttc ttaggttttg
    38521 aaggtgataa agaaccagat atagacttaa atttctcagg aaattatcag ccaacaattc
    38581 ataaatacac agaagaacta tttggagagg ggtatgtata tagggctggg actataggta
    38641 ctgtagcaga aaaaacagct tttggatacg caagaaaata tgtggaagaa aataatataa
    38701 gtgtaccaaa tgcagaagta cttaggcttt caaatggatg tactggagta aaaagaacct
    38761 caggacaaca tcctggtgga gttatggtta taccagacta taaagatgta tacgatttta
    38821 cacctataca gtatccagca aatgatactt cttgtggtgt tataactact cactttgatt
    38881 atcattcaat aagtggtaga atacttaaac ttgatatact tggacacgac ggtcctacca
    38941 ttataaggat gcttgaagat atgactggaa taaatataac agaaattcct ttagatgata
    39001 aagagactat gtctctattt acatctacag aagcattagg tgttacacca gaagaaataa
    39061 actgtcctat aggttgcctt gctgtaccag agtttggaac taagtttgta cgacaaatgt
    39121 tacttgacac aaaacctact acatttgcag aacttgtacg tatatctggt ctctctcatg
    39181 ggacagatgt ttgggtaaat aatgcacaag acttagttag agaagatata gttggattaa
    39241 aagatgttat atctacacga gatgacataa tgaactattt aatatttaaa ggattacctc
    39301 ccaaaatgag ctttacaata atggagagtg taagaaaagg taaaggttta aaaccagaac
    39361 acattgaaga aatgaaaaaa aataatgttc cagaatggta tataggctca tgtaaaaaga
    39421 ttaagtacat gttcccaaaa gcacatgctg ttgcttatgt aatgacatca tttagaattg
    39481 catattgtaa agttcactat ccagaagcat tttatgcgac ttattttacg acaaaagttg
    39541 aggattttga tgcagattta attgttaaag gattagatgc tataaaatca aaaataaatg
    39601 aaattgagtc actgggaaat gatgcaacca caaaagaaaa aggtatgttg accgttttag
    39661 aagttgctct tgaaatgtat gctagaggaa taaaattatt accagttgat atatataaat
    39721 cagatgcaac tgagtttata gttgtaggag aaaaaacatt acttccacca atggcagcaa
    39781 tacaaggtct tggaggaaat gcggctataa acatccaaaa tgaaagaaaa aatggtgaat
    39841 ttatatcaaa agaagatttg agaaagagaa caaaaatatc aaaaacggta atagaaactc
    39901 ttactaatca tggttcactt gagaatatga gtgagagaaa tcaattgtca ttgttttaag
    39961 ttagcccaaa aagttgcaaa taaaagaatt ttatgatatc atagtataga aaatagtata
    40021 ctttgcagaa actaagagtg agacagctgt tctcactctt ttattttaaa tatattatta
    40081 aaaagctggt ttatttttat taaatatatt ttatttaaga ttatataaac tggtcattta
    40141 caattgaata ggaggaactt taatgaaaaa aaatttagaa gctacaattg aggagattgt
    40201 aactaaaata acggatgaac atggatttga aatggttgat gttgaatatg taaaagaagc
    40261 aggagaatac tacttaagag tatacataga taaagaagaa ggaatatctt taaatgaatg
    40321 tgaattagtt agtagagaat taagtccaat actagatgaa aaagacccta taaaagaaaa
    40381 ttacttttta gaggtatctt ctccagggct tgatagagca ttaaaaaaag acagagattt
    40441 tgttagatat caaggtagag atgtagattt aaaattatat aaaccattaa atggatgtaa
    40501 gcaatttgaa ggtgaacttg taggattaac agaagataat aatataaaaa tcattgcaaa
    40561 tggtaaggaa atggaattta atagaaaaga tgttgctatt gtcagattag caataaagtt
    40621 ttagtggtat attaaatagg gaggatataa aaaatgaatc atgaatttat ggaagcatta
    40681 gatgaattag tagttgacag aggtatagat aaagaaatac ttatagatac tatagaacaa
    40741 gctcttttaa ctgcatacaa gaaaaacttt ggttcagcac aaaatgttag agttgaattt
    40801 gatagagaca agggagatat aaaagtattt tctcaaagag ttgttgttga tgaatcagat
    40861 ttatatgata ctttcttaga aatagagtta gcagatgcta gagagataaa ccctaattat
    40921 gaattaggtg atattataga acatgaagtt acacctaaaa actttggaag aattgcagct
    40981 caaacagcaa agcaagtagt tgttcaaaga ataagggaag ctgagagaga aatagtgtat
    41041 aatgaattta tagaaaaaga aaatgaaata gttacaggag aggttgctag agcaaataaa
    41101 aatgttgttt atgtaaatct tggaagaata gaaggtataa tgactcaaag tgaacaaata
    41161 ccatttgaga attataaagc aggtcaaaaa ataaaattct atatacttga agttaaaaag
    41221 actaacaaag gacctcaaat agttgtatca agaagtcatc cgggtctagt aaagagattg
    41281 tttgaagaag aagttcctga aatatatgaa ggaatagttc aaataaaatc tgtagcaaga
    41341 gaagcagggt caagaactaa aatggcagta agatctatag atgaaaaaat agacccaatt
    41401 ggagcttgtg ttggaccaaa aggacttaga gtaaaaaata tagtggatga acttggcgat
    41461 gagaaaatag atataataaa atatagtgaa gacccagcag agtttatatc agcagcactt
    41521 agcccatcaa aggttgttag agtagatata gatgaagatg aaaagtctgc cttagtaatc
    41581 gttccagatt atcagttatc gcttgctata ggtaaagaag gtcaaaatgc aagactagca
    41641 gcaaaattga ctaactggaa aatagatatt aagagcgaat ctcaagcaga acaagaaatg
    41701 ttaaatcttg aaaattccaa agaaatagaa gctgaggatg ttgaagctga atttatcgac
    41761 caagaagagt tagatacttc agaggataat tttagtgaag aagagtaagg agggatgatt
    41821 ttgaaaaaag taaaaaaagt gcctcaaaga aaatgcatag catgtcaaga tagagactct
    41881 aaaaaagagc ttataagaat agttaaaaat aaagaaggaa aaatattttt agacccaaca
    41941 ggaagagcca atggaagagg agcatatgta tgtaaaagct ctgaatgtct aaaaaaagct
    42001 ataaagagta aagcattaaa taaagctttt aaaatagatg ttcctgatga agtctatgat
    42061 aatctactta tggagttaga agaatatgaa aaataataaa gaaaaagtac tttcgttctt
    42121 agggctcgca actcgtgcag ggaaaatagt gtctggtgat gattcaactt tgcttgacct
    42181 taaaaaagga aaagtaaatt tgattttaat cgcagaagat gcatcaaata atactaaaaa
    42241 actttttaaa gataaatcaa cttttagaaa tattccatat ctattttttt caacaaaaga
    42301 ggaaatagga tttgcaatag gaaaatctcc aagagcagtt gtaggaataa aagatgaaaa
    42361 tttttctaaa aagataattg aactaataga aatttaaaat aaaatagggg gtgatcttgt
    42421 gtcaaaaaca agagtatacc aaatagcaga agaattaaat atatcaaatg aagagcttat
    42481 aaataagtta gctgaattag atataaatgt aactgacaaa gattcagtat tagaaggtga
    42541 agagttagaa ttagcacttg aaatgttagg tgaagattta tctcaagaaa atggaaatgt
    42601 tatagaaata gatggaaagt tgacagtaca agtattagca acgaaattag ataaatctcc
    42661 atcagaaata attatgaagc ttatgaaaat gggaactatg gcaacaataa atcaagagat
    42721 aagttttgag attgctgctt tggctgccaa agactatgga tttgaattaa cagtagctga
    42781 aagtgatgat acagaagctt tagaaataga agctttaatg gaaattgaag aagataaaga
    42841 agaggattta aaacctagac caccagttgt tacagttatg ggacatgttg accatggtaa
    42901 gacatcttta ctagatgcaa taagaaaaac tgatgtgatt tcaggtgaag ctggaggaat
    42961 aactcagcat ataggtgctt cagaagttaa aatcaatgga cacaaaatag tatttttaga
    43021 tactccagga catgaggctt tcacatcaat gagagcaaga ggagcacaag ttacagatat
    43081 agcaatatta gtagttgcag cagatgatgg tataatgcct caaacagttg aagctataaa
    43141 ccatgctaaa gcagcaggtg tacctttaat agttgctatt aataaaatag ataaaccagg
    43201 tgcaaaccca gataaggtta aacaggaatt agcagaccaa ggattattag ttgaagactg
    43261 gggtggagaa gtaatagcag ttccagtatc agctaagaaa aaagaaggaa tagatacact
    43321 tttagaaatg gtattattgg ttgctgaaat ggaagagtta agagctaatc caaataaaag
    43381 agcagtagga actgttatag aagctgagct tgataaagga agagggccag ttgcaacagt
    43441 acttgttcaa ggtggtactt taacagtagg tgacccaatc gttgcaggtg ttgcatgtgg
    43501 taaagttaga gcaatgataa atgcaaaagg taagagagta aaaacagcag gaccatctac
    43561 agcagtcgag atattaggtc tatcagaagt tccacaagga ggagaccaat ttgtcgaagt
    43621 tccaactgat aagattgcta gaagtgtagc agcaagaaga caacaaatag ttagagatga
    43681 aatgttaaaa tcaactcaaa gattatcact agatgctcta tttagtcaaa tgagtgaagg
    43741 ttctataaaa gaccttaata tagttataaa agcagatgta caaggttcag tacaggcagt
    43801 taaacaatct ttagaaaaat tatcaaatga agaagttcaa gttaaagtaa tacatggtgg
    43861 tgttggagct gtaacagaat cagacatatt acttgcagca gcttctaacg caataataat
    43921 aggattcaat gtaagaccag ttccaggtgc agaatctcta ggagaaaaag aaaatgttga
    43981 tataagaaca tatactataa tatacaaggc aatagaagat atacaagcgg ctatgactgg
    44041 tatgttagac cctgaatatg ttgatgaaga aacaggaaaa gctgaaataa gagaaatata
    44101 taaaatatct ggtgttggaa cagtagctgg atgttatgtg actaatggta agatatttag
    44161 aaattgtaaa gtaagattag ttagagatag cataataata catgaaggtg agcttgcagc
    44221 acttaagaga tttaaagatg atgtaaaaga agttaattct ggatacgaat gtggtatgag
    44281 ttttgtaaat tataacgaca taaaagaagg cgatatagta gaagcttata taactaaaga
    44341 agtagaaaga aaattataaa ttcgtatcaa agaaaatcag ttagtaaaga aggtcgatgg
    44401 tatggcatca tataatagaa caagaaggat agcagaagaa ataagaaaag tcgtaagtac
    44461 tatgcttata aatggagtaa aagaccctag aataacatct atggtgagtg taactgatgt
    44521 agaagttaca aatgacctaa gatatgctta tgtatatgta agtattctag gcggagatga
    44581 agaatccaca ctaacaggtc ttaaaagtgc tggaggatat ataagaagag aagtaggaaa
    44641 aaatataaaa cttagatata ttcctgagat agtatttaaa cttgatgatt caatagaaaa
    44701 aggaatgtat atggatagcc ttataaaaag agttaatgaa aaaaatgctc aacaaaatgg
    44761 ggatgaaaat tatgatgaat aatatggata gtattataaa ctatatttat gagggtgact
    44821 attttatagt tacctctcat atcagtccag atggagacaa tattggttct acactttcaa
    44881 tgtattatac attgaagaat ttaggaaaaa atgtatatta tgtacttgat gatgaagctc
    44941 cattaaattt aaggtttttg gttgaaggtg taaaaatcta taagtctaat gaatttaata
    45001 tgaagaacta ttctctaatt gcacttgatt gtggggataa gatgagaata tgtgtgagtg
    45061 atgaaatcaa aaataatgca tcaaaattaa tctgtattga ccatcatgct agtaatgatt
    45121 catatggaga tttaaattat gtagatattg atgcatcttc tacatgtgaa ttagtttata
    45181 atttattagt tagatttcag caaataaaag atattaatat tataaatgaa gatatagcaa
    45241 catgtttata tacaggtctt gtaactgata ctggaaactt ttcgtattca aatgtacatg
    45301 caagtagctt tgaaatggca aaaaatttac ttgttttagg tgcacaaaaa aatactataa
    45361 tacaaaatat atatcaaagc aattcttctg gttattacaa attattagga gaagctctaa
    45421 agggtttgga gatatttgat taaaaaatag ttacgcacac tcgtgacttt agtcatgagt
    45481 taggagctaa aatagtcagc atatagggaa acttatatgt agagatagga taaaacctat
    45541 ccaatactac ttgaattgct ggaaacccct aaagctaacc aaactaaaac ataaggatga
    45601 aataaaccta agtgtgaaag ttacgaaagt agaaaaaatt ggttagatgg tataaggtta
    45661 aaccctaagt ac
//
LOCUS       DBGJFF010000015        45401 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.015, whole genome shotgun sequence.
ACCESSION   DBGJFF010000015 DBGJFF010000000
VERSION     DBGJFF010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 45401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45401
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.015"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(167..1525)
                     /locus_tag="ACOIAF_000918"
     CDS             complement(167..1525)
                     /locus_tag="ACOIAF_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008624114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HGZ2667940.1"
                     /translation="MVNVGNILETIKMIDEEQLDIRTITMGISLLDCIDPDGDKAREK
                     IYNKIMSSAKDLVKVGKDIEREFGIPIVNKRVSVTPISIIAGATDENDYVKFAQTLDK
                     AAEDLGIDFIGGFSALVQKGYTKGDKILIKSIPKALASTNKVCASVNVGSTRCGINMD
                     AVKEMGEIIKETAELTKDAKGFGCAKLVVFCNAVEDNPFMAGAFHGVGEADRIINVGV
                     SGPGVVKRALEKVKGEPFDVVSETVKKTAFKITRVGQLVAKEASSRLDVPFGIIDLSL
                     APTPAIGDSVANILEEMGLEVVGTHGTTAALALLNDAVKKGGVMACSHVGGLSGAFIP
                     VSEDAGMIDAVIKGALSIDKLEAMTAICSVGLDMIAVPGNTTAGTLGAMIADEAAIGM
                     INNKTTAVRIIPAPGCDVGDMVEFGGLLGRAPVMPINTNSSELFTQRGGRIPAPIHSF
                     KN"
     gene            complement(1548..1817)
                     /locus_tag="ACOIAF_000919"
     CDS             complement(1548..1817)
                     /locus_tag="ACOIAF_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HGZ2667941.1"
                     /translation="MKAILTVIGKDKPGIVAGISDELYKQNINIVDISQKILQDYFTM
                     IMIVDLEKSLNSFEKTVEDLVERGKKLSVDVKIQREEIFNSMHNL"
     gene            complement(2107..4077)
                     /locus_tag="ACOIAF_000920"
     CDS             complement(2107..4077)
                     /locus_tag="ACOIAF_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667942.1"
                     /translation="MKKYEIFIFMSIIVLCFISFPHKVFGQEENCLEDFERWVQENKN
                     NKEEIVYTLSCDMVIDEEFRFYIPYDSNFTIDTNKYKILIKNHGRFIIDDNELNIIGE
                     GGKEGVIHIENGGSISIGINNIIATDGTALYVEEGGDLHIKSSYESVEKIKANGKDAI
                     GIYTENDIRILNKDIEVNGEHAIGVYSKGDVEIEETSIKAYSSNNESGLLRDNNKLAQ
                     SIISESKKVYIIDDYNELIPSIAEDSGYDIVKCCHRGIGVFSDEIKVSKDDKIENIKF
                     PKYMVLETSSGNRLDIDVEWDFTDYYEKLEKGENFNITGKFKTEMLNKEKIIINDDVV
                     PILNVSVIEKKPIDNLELEFQNTKNGYVAVLFYDMPYSASKVFVEYSSDGINWISEEQ
                     EDIRDQAILFFEDFKLRCFRVKVVGGLKEGYSNIVFKPGFIMGGGDNQETPDDEREND
                     DIDGDRGGGGRDDPDRDEDDNNQDGNNGNENNDSNNNQNNNNENNNNENNNNENDNSD
                     EKQPNTPKDDVESSTDSNENKNPHDIPKDGNKENNSDKEKGFYNKNSITENDDNYDLS
                     SKSVFGTNEHSFPYRESISNKNQDTNKNKSNISDYDGIINNNYKLESKEYNLKKDIIF
                     LFCILTPIMIGSILIVNPDTRKSIIKFIKLKK"
     gene            complement(4261..5736)
                     /gene="spoIVA"
                     /locus_tag="ACOIAF_000921"
     CDS             complement(4261..5736)
                     /gene="spoIVA"
                     /locus_tag="ACOIAF_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HGZ2667943.1"
                     /translation="MNNNIYEDISKRTQGDIYIGVVGPVRTGKSTFIRKFMEKLVIPN
                     IDNEFKKDRTRDEIPQSGSGKTIMTVEPKFVPADGVEIKIKDTVSLKVRMVDCVGYIV
                     EGALGHEEGGKQRLVSTPWSQEAMTFEKAAEIGTKKVIKDHSTIGIVVLTDGSVTGID
                     RKSYVEPEERVIQELKNLKKPFAVVLNTLSPKSEETSMLRSELEEKYEVPVLPMNVVE
                     MEEEDIEEVMEAVLYDFPLTEIRINLPKWVEGLERNHWIKSSIITTLKQSIIDIGKIR
                     DIEGIIQGFSELEFLEDTGVDNVELGEGVINIDLQTKQDLFYNVLEEKSGFKIEGDYQ
                     LLSLITRLSKVKNEYDKIESALIDAKIKGYGVVAPSLEELSLEEPEIMKQGKQYGIKL
                     KANAPSLHIIKADISTEVSPIVGNQNQGEEMIKYLMEVFEEQPADLWESNMFGKSLHD
                     LVKEQLQSKLYTMPEEIRVKMQKTLQKIVNEGSSNIITILL"
     gene            complement(5908..7203)
                     /locus_tag="ACOIAF_000922"
                     /pseudo
     CDS             complement(5908..7203)
                     /locus_tag="ACOIAF_000922"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
     gene            complement(7387..8832)
                     /locus_tag="ACOIAF_000923"
     CDS             complement(7387..8832)
                     /locus_tag="ACOIAF_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HGZ2667944.1"
                     /translation="MSLNKFEIGGITVKKKIGFWDLVFMNISALFGIRWIAKSTASSF
                     GLGLGSIPVWLLFAFIFFVPASLICAELAATYPKDGGLGEWVKQAYGEKWGFMVSWLN
                     WTSKIFWYSSFLTFLAINIAYMLGNPDLSNNKMFILALSLIIFWLLSLVSTRGMAFGK
                     FFTNTGALGSTIPAILLIVMAFMSVVILKKAPSASIYTIQNIIPKIDANSLVSISAII
                     FALSGAETTANFITEMDNAKKNFPKAILTMAVLIGGIYILGSVAITMILPPDKIAAST
                     GILDALAKVAQDLGIGSWFIRIVAFGITLSVLGALILYIAGPVKMLFGNVREGVFPKQ
                     LTVTNKYNIPSNAVIVQAIIVSLLLVGTNLLPSVDNIYNVLVTMTALTALFPYVLLFT
                     SYIRLRKTRPNEERPYSIAKSDSVCINIARMVLVVTVVGILFSTAPVMETLKDNIIYE
                     IEMIGGGLFVIITGLMLWKNYEKKISNNSGR"
     gene            complement(8848..9987)
                     /locus_tag="ACOIAF_000924"
     CDS             complement(8848..9987)
                     /locus_tag="ACOIAF_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HGZ2667945.1"
                     /translation="MSNNMRLERLRKYMNEKSIEIALIFEPDNQFYISGFKAITYSRP
                     IVTVVTQDKIELIVPGLEELHAKEVAKVDNVYVYYEIPEMKEHGISHKHYLDIILNKY
                     PKGTTVGIEKDIVSASFSEYITDKSFAIKDVGSKIFEMRYVKDAKEIEFLKIAGYLSD
                     IGIKGSLENVRVGMSELEFDVAGDNALLKYVSENYPDTYIGFANWTCSGIDRTAQPHL
                     DSNTRILQRGDIVIHSRQVWYENYRAENERTFIIGKPTERQKEVFKIAVEAQQAGLDT
                     IKAGIPARMVDEAARAVVAKYGLELYSNHRIGHGLGLSEHEEPYLRFDNELILEEGMV
                     FSMEPGIYIPGVGGFRHSDTAIVGKNGATIITNYPRSVEELILDI"
     gene            10306..10956
                     /locus_tag="ACOIAF_000925"
     CDS             10306..10956
                     /locus_tag="ACOIAF_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HGZ2667946.1"
                     /translation="MTNKELQKKRILMYFIEAAQNIMENEGIENITLRKVADMAGYNS
                     STLYKYFKNLDHLISFASIKYFKDYNLNISRCIENVNDEYKKYIIMWKLFCKHSFDNA
                     QAFYQVFFNLSSDELSYITKQYYDMFPEDLGIHDSDISLMITGESIKERNKILLNNLV
                     DTGYIPAKDLDIINDIIISFYQNLLFLKKNTGKNSDDDELTMKMISSIEYIIKHAK"
     gene            complement(11113..12132)
                     /locus_tag="ACOIAF_000926"
     CDS             complement(11113..12132)
                     /locus_tag="ACOIAF_000926"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HGZ2667947.1"
                     /translation="MEKVCVLGTGSWGSALALGLAKKGNDVSMWTRKEEQAKKINRTK
                     ENTDYLPGVLFPNNITISTDIEKTIKDCKVIVLAVPSQAVRSTCQKIKPFIKEGQVIV
                     NVAKGLEKGTGLRLSQVCEEELPQNPYVILSGPSHAEEVARDIPTTVVVASKDLKIAQ
                     MIQDLFMSPKLRVYTNPDIVGVELGGALKNIIAFGAGICDGLGYGDNAKAALMTRGIS
                     EMSRLGIAMGANMSTFAGLSGIGDLIVTCTSMHSRNRRAGILIGKGMSLEDTLKEVKM
                     VVEGITATEVAHDVSEKLDIDMPITNAIYSVIKKGSNPKEVGIELMMRSKKHEMEEVV
                     LGDDI"
     gene            complement(12163..12777)
                     /gene="plsY"
                     /locus_tag="ACOIAF_000927"
     CDS             complement(12163..12777)
                     /gene="plsY"
                     /locus_tag="ACOIAF_000927"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HGZ2667948.1"
                     /translation="MEIFSYIIIAVVAYLLGNISTSYIVAKRIAGVDIRTQGSGNAGS
                     TNVLRTLGKRAGAMTFLGDVLKGVMAVLISEFAARLVGIDTLLAGYLAVICVVAGHNW
                     PAVLGFRGGKGVATSLGAMLAVNPVITLMCLAVFILVVAITKYVSLGSVVGIGCSPIF
                     MIMVKNKAGLIVALFLTASVIYNHRANIKRLLNGTERKIGQKKE"
     gene            complement(12812..14137)
                     /gene="der"
                     /locus_tag="ACOIAF_000928"
     CDS             complement(12812..14137)
                     /gene="der"
                     /locus_tag="ACOIAF_000928"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HGZ2667949.1"
                     /translation="MSISRPVVAVVGRPNVGKSTIFNKFAGKRISIVENTPGVTRDRI
                     FAEVEWLDKYFTLVDTGGIEPDSEDIILSQMRNQAMLAMDMSHVILFIVDGKAGITAA
                     DKEIAQLLRKTKKPVILVVNKIDSQSQFDNIYDFYELGFGTPFAVSGANSMGFGDLLD
                     EIVENFPAGLDTEYEEDIIRVAITGKPNAGKSSILNKILGEERVIVSPIAGTTRDAID
                     TYFEKNGQKFLLIDTAGLRRKSKIYETIEKYSVIRAMSAVDRADVVLIVIDALEGVTE
                     QDTKVAGIAHDEGKGCIFVINKWDLIEKDNKTMSNYTKDIKEKFPFMMYAPIVFVSAK
                     TNQRMNKILDTVEYVSNEHSKRISTSALNEVIGEAVMLNQPPSDKGRRLKIYYGTQTD
                     IRPPKITLFINDKDLTHFSYQRYLENKIRENFGFEGTSIKFEYRQKNKK"
     gene            complement(14165..15493)
                     /locus_tag="ACOIAF_000929"
     CDS             complement(14165..15493)
                     /locus_tag="ACOIAF_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HGZ2667950.1"
                     /translation="MEVNVKKVNNIISKVYKDSIAEEMGIEVGDLLISVNEQPIHDII
                     EYRFLLSDEYLDVEIQKKDGEVYIYEIEKDYDEDLGVEFTNPIIDQAKSCRNKCMFCF
                     IDQLPEGMRETLYFKDDDSRLSFLQGNFVTLTNMSEEDINNIIKYRISPINISVHTTN
                     PELRQKMISNKFAGKLYGIMKRLADAHIEMNCQIVLCPGINDGKELDRTIKELAQLYP
                     YVNSVAIVPVGITKHRENLVELNIFNDKSASKTIEQIHQIQQKYLEKLGTRFAFLSDE
                     FYILSNSELPGYEEYEGFLQFEDGVGMIRKLKTEIEEYLNILPENILKREKKVSIATG
                     HSAYEFIQSMADAMMDKFKNLQVNVYEIKNKFFGETITVSGLLTAKDLKEQLEDKELG
                     EALYITRSMLKADEEIFLDNIELNQLEELMRIKIIPCLNEGKDFVDKILK"
     gene            complement(15731..15931)
                     /locus_tag="ACOIAF_000930"
     CDS             complement(15731..15931)
                     /locus_tag="ACOIAF_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667951.1"
                     /translation="MGMDIGLNEYTLAIVTLDKNMKNGGGCPIFYANDEKDLQNKALL
                     MSKCVGGMVHDMTNGTLIIVKH"
     gene            complement(15933..16865)
                     /locus_tag="ACOIAF_000931"
     CDS             complement(15933..16865)
                     /locus_tag="ACOIAF_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HGZ2667952.1"
                     /translation="MDRVFFVAVIIGITSRIIMLNLDQKQYPTQPNVLLSQLILAFVA
                     SSLGALLVPALINRSYTSITFLSLAAEQFRQVRANRRNTLQNLEEVQLVKRGAAFIEE
                     IARTYEVRNYMCIVTSFLTIGLYYVILAETKISEGMSLVISSICGLVLAFILKKLLTR
                     KSIGDIADVMPAEISFVDESILKVGDLKGITNIGLKSDREVYINRGVGIQIIPKNKDY
                     ANAGILFDSGQRQAIVYNIYSRLGLYGDYGEPSFVPLPRRNKEDESIMIAYLPVDKNV
                     EKVMEAVKSCPILSSSKGKNLSLKNYKIGKKGSA"
     gene            complement(16910..17842)
                     /locus_tag="ACOIAF_000932"
     CDS             complement(16910..17842)
                     /locus_tag="ACOIAF_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HGZ2667953.1"
                     /translation="MEESLLNANLFRHSFIVALVVGILCRGLVLRVTDKQYPSRPQDY
                     LEQIIISGLSASLGAIALPALIDKEFAALTFFAVAIQQFQGLAEQERITLKNIDNEEL
                     VPKGDAYVEEIASTYESRSYISLFSALVSSIVYIVFARKYGLSFFYCTILAIVSGAIV
                     GLIFRRFLRRNSIADIADIVPAKISFEGPILMVNGVIITNIGLESTREKYRNECLAIE
                     VIPRDLGAFGIVNDIGQRQAIIHNLFIHMGIDRDVDEVDIVAISKTNLEKSTVVIPYM
                     PILKDIDVMIDVVKSTPIIETSKGKQSDFSRKTL"
     gene            complement(17897..19186)
                     /locus_tag="ACOIAF_000933"
     CDS             complement(17897..19186)
                     /locus_tag="ACOIAF_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGZ2667954.1"
                     /translation="MEDLNSIYFFIVMLSIAIVILAVRYTITLRKYLKEFIYVSKKVS
                     NNEYHARLNISAKGELGELARNFNHMIKNMDKTLEEVQYKHLQLRSILKSISHGILAI
                     DVNGNILLINDEAKEMIKSEPNQLVEGHNINVVIKEEEILKQILQFIGSKDNETANIT
                     TKDDIAYKIKVDPVYLQNTNNVIIGSIVNIEDITKKVKLENMRSDFVANVSHELKTPL
                     TSISGFVETLKSNENIDVNTRNRFLEIIESESDRLKRLIDDILLLSFVENKDTLLMEN
                     VVVYDVFKEVYEITQHSASEKNIEVLYKCDDKSISILSNKDYIKQVFLNLVDNAIKYT
                     QDNGIVQVVVLKDEEKIVIKVIDNGVGIPKEDVERIFERFYRVDKARSRDVGGTGLGL
                     AITKHIVKSLNGCIKVKSELGIGSEFIVTILQKNFLK"
     gene            complement(19215..19907)
                     /locus_tag="ACOIAF_000934"
     CDS             complement(19215..19907)
                     /locus_tag="ACOIAF_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGZ2667955.1"
                     /translation="MNTKVLVIDDEMHIVELLKFNLEVSNYEVSYSYDGFDGFIKAKE
                     IKPDLILLDWMLPNISGIEVLRKIRSDKDLKNIPVIMLTAKNMENDKVEGLEIGADDY
                     ITKPFSIKELLARISSVLRRYNLTSLGEENNILTTGNLKLDLSKHEVTKGSEKIELTL
                     KEFELLKLLIQNKGKVLSRNYLLDKIWGYEYYGETRTVDVHIRYLRKKIEDEDKSEKY
                     IETIRGVGYKID"
     gene            complement(19922..20683)
                     /gene="pgeF"
                     /locus_tag="ACOIAF_000935"
     CDS             complement(19922..20683)
                     /gene="pgeF"
                     /locus_tag="ACOIAF_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HGZ2667956.1"
                     /translation="MMKDYINIKNVQDIKEYVNLSGNCEELDEKLNSVNLVITCKNID
                     AKNREDMLKVCKECDLTFENLTSNSQIHSDIVNIVNKDTIGKRRDGDALITNLEKVPL
                     LLFTADCVPISIIDAKNKAIGLAHAGWRGTFSNIGHKTIKLMSECYKTVPEDLVCIIG
                     PSIGPCCYEVSEDLVEKFNTILTNNDEKFYIIKEGSYYLDLWKINEYMLRCSGVKKEN
                     IINLNLCTSCRADKFHSYRKHNKASERIGTVLQIK"
     gene            complement(20680..21141)
                     /gene="nrdR"
                     /locus_tag="ACOIAF_000936"
     CDS             complement(20680..21141)
                     /gene="nrdR"
                     /locus_tag="ACOIAF_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HGZ2667957.1"
                     /translation="MQCPYCNYKESKVIDSRHTDLKSIRRRRECESCKKRFTTYEKIE
                     TTPLMVIKKDNSREYFDREKIKYGLLKACEKRPVSIDEIESIVVHIENEINKCFIEEI
                     ETKKIGEMVMDKLKELDEVAYVRFASVYRQFKDINTFVNELKSILIEKGDK"
     gene            complement(21163..21426)
                     /locus_tag="ACOIAF_000937"
     CDS             complement(21163..21426)
                     /locus_tag="ACOIAF_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HGZ2667958.1"
                     /translation="MIRVSDIMEKEIINVKNGKRMGFIIDIDMDIHEGKVVSFTIFGD
                     GSRSFFSRGGDEEVIFWNDILKIGCDTIIVNIGSELSLDEFEI"
     gene            complement(21530..22303)
                     /gene="sigG"
                     /locus_tag="ACOIAF_000938"
     CDS             complement(21530..22303)
                     /gene="sigG"
                     /locus_tag="ACOIAF_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigG"
                     /protein_id="HGZ2667959.1"
                     /translation="MQVNKVEICGVNTSELPVLKNKQMKELLLQIKNGDEEARQQFVR
                     GNLRLVLSVIKKFNNRGENIDDLFQIGCIGLIKAIDNFDLSQNVRFSTYAVPMIIGEI
                     RRYLRDNNPIRVSRSLKDIAYKALQVRERLIRTNSKEPTVSEIAKELELEVESVVMAL
                     DAIQDPISLFDPVYQDNGDAIFVMDQVQDKKDTDENWLQEISLKEAIKKLNSREKLVL
                     DLRFYKGRTQIEVADEIGISQAQVSRIEKNALKNMRKYV"
     gene            complement(22395..23117)
                     /gene="sigE"
                     /locus_tag="ACOIAF_000939"
     CDS             complement(22395..23117)
                     /gene="sigE"
                     /locus_tag="ACOIAF_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigE"
                     /protein_id="HGZ2667960.1"
                     /translation="MLRLKERIISFITMLGIKLKIIKPKGIYYMGGANILPPPLKPEE
                     EMELLQKLETDESVKSILIERNLRLVVYISRKFENTGIDVEDLISIGTIGLIKAVNTF
                     KLNKNIKLATYASRCIENEILMYLRKNNKKKTEVSFDEPLNIDLDGNELLLSDVLGTE
                     NDEIYKIIEEEIDRDLLVMALDRLSDREKQIMELRFGLIDKGIEKTQKEVAGMLGISQ
                     SYISRLEKKIISRLQKEMKKFV"
     gene            complement(23142..23987)
                     /locus_tag="ACOIAF_000940"
     CDS             complement(23142..23987)
                     /locus_tag="ACOIAF_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E processing peptidase SpoIIGA"
                     /protein_id="HGZ2667961.1"
                     /translation="MVYIEYYVIENLVINYIIISCTSILTKRYNPKKNKLLGASLGGA
                     YSVAYLYPSMNILFTVPFKIIIMSIITLISFRSKSKKDYVHVALVFYLVNIFISGSTY
                     FIIYFTGISHLKISFLIACTYVSCELLKYIYKDLKMLKYIKELTKTININLLEKSFCC
                     KALVDSGNLLKDPISQSDVIIVKSSVLEGFIPEKLLNINYEDIDMKKAQEIIGSLDDK
                     TSGRVRIIPYKHAGSNKTSIIIGLKADYIEVDEQKIGNIILGISNFNDREYGAIMNPN
                     ILPGF"
     gene            complement(24304..25578)
                     /locus_tag="ACOIAF_000941"
     CDS             complement(24304..25578)
                     /locus_tag="ACOIAF_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HGZ2667962.1"
                     /translation="MIKLKKVLSLVMVGILSVSILAGCSKKEDSKGKEGKEKLVVWSA
                     PLTDHDADAWKPIFEKFEKENNCEIEFQIVPWDNYAEKYATAISAGEGPDIGYMYAEM
                     FPQFIEMGAVEDLTPYLEKSGTSDNYLYLDDAKMMGGIYGLPIEAANPGVLYYNKDIL
                     EKLGEKPPKTWDDFKRICEKATKDTDGDGKIDQWGLAQGWGSKVFGSLNWNWYPYLWQ
                     AGGDIYNDDLKSVKFNNAEGLKAANFINDMKKYIPEDALSKDSNEMIESVFGPGKAAF
                     TIMLSSAATSTFDKSFPDLNWGYVTSLEDKTVGSFASVDDLTLMSACKNKELGFKLLE
                     YMLSKESMEAFHKEIPRAPVAKGEKYQGDPRFEEMVTRDKHMYRPLTVGPHGVEIYEY
                     LWKQLQMMIAGDITPEQALNESAKYANGLLAQ"
     gene            complement(25862..27019)
                     /gene="ftsZ"
                     /locus_tag="ACOIAF_000942"
     CDS             complement(25862..27019)
                     /gene="ftsZ"
                     /locus_tag="ACOIAF_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HGZ2667963.1"
                     /translation="MLNFDVELEECAQIKVIGVGGGGNNAVNRMVEAQLKGVEFISVN
                     TDKQALYTSKAEYKVQIGEKLTRGLGAGANPEVGKRAAEESKDEIVKLLQGADMVFVT
                     AGMGGGTGTGAAPVVAGLAKEMGILTVGVVTKPFAFEGKIRMKNAEGGIAELKSKVDT
                     LITIPNDRLLQIVQKNTSMLDAFAVADDVLKQGIQSISDLIAVEGLINLDFADVTTIM
                     KDKGLAHMGIGSASGETRAIDAARQAIQSPLLETSIQGAKGVLLNVTGGPNLGLFEVN
                     EASTLVMESCDPEANVIFGASIKEDLGDEIMITVIATGFEGLQNGALDLDTKPKSSIR
                     SSLNTTVKQAVKEIEEEVIAEEKIEPPKKASIIEEDDDESMEIPTFLRRRR"
     gene            complement(27209..27562)
                     /locus_tag="ACOIAF_000943"
     CDS             complement(27209..27562)
                     /locus_tag="ACOIAF_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HGZ2667964.1"
                     /translation="MLWVLGGLAFGVVVGYYIPFTYSMDYSIYMSVAILAIMDSIFGA
                     IKSNFEDNYDNVIFITGFIGNAILAILLTYIGEKLGVSLYYVAVLVFGMRLFNNLSII
                     RRYIISNIMNKKRKN"
     gene            complement(27661..28335)
                     /locus_tag="ACOIAF_000944"
     CDS             complement(27661..28335)
                     /locus_tag="ACOIAF_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HGZ2667965.1"
                     /translation="MKKKIVILGAFILGVFISTYIKSLDPNRVYITLGQTESIESEVE
                     NTKKEIKNLKETLKKNKKTLNEYKEAKKNGNESIQTLMQKELEEVKELSGYSTVTGPG
                     ITITMRDSERELKDGQNPNDLIIHDIDILRVLNDLKKAGARAISINGERVLATSKIKC
                     SGATITVNDTTYGQPFIIKAIGDIDLLSAAINSPESYAKSLKDIYGINIKVQENNGNK
                     VNLFAK"
     gene            complement(28340..29038)
                     /locus_tag="ACOIAF_000945"
     CDS             complement(28340..29038)
                     /locus_tag="ACOIAF_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HGZ2667966.1"
                     /translation="MKKRFINKSIIIGSIVLGVLVSLQLKIINIENRGMTTFKRGEQL
                     TEELKSLKKEKENLQSEVNSVKKDIETYRGKNNEGEQDILKSEIEKYETLAGYTDVEG
                     TGIKIEINSTKDLGNSDKNDSILYNYDLLLSMINKLNSAQASAISINDQRIVSNTYMY
                     LKEDKLYMNDTVIKEPIIIKAIGDSDTLASALNIKYGIVWEIETYYNAKVKVTKEDNI
                     EINGHSDINKAEGK"
     gene            complement(29061..29801)
                     /locus_tag="ACOIAF_000946"
     CDS             complement(29061..29801)
                     /locus_tag="ACOIAF_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HGZ2667967.1"
                     /translation="MKKRRKLNTNNVMIVLVFILMLSFCICIIVKSDLFDVKKIDVIG
                     NKRVTKSNIMKELNVNLNENIFAYNFKDMKNKLIKNPYIENVEIKRKLPNKIIISLKE
                     KEIFAVLKDEDNYCYIDKKGNLLEELRGSNESKKDLIVDVDYSIDDNKSIKFKNYKTK
                     KNVFKTLNYLKEEGIYRKINYVNLKKESNIEMLTRSNIKILLSNDDNLDYNISRVSKI
                     LIDLQNKNTNGGTINLNYGKLAVYSPEG"
     gene            complement(30071..31297)
                     /gene="murG"
                     /locus_tag="ACOIAF_000947"
     CDS             complement(30071..31297)
                     /gene="murG"
                     /locus_tag="ACOIAF_000947"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HGZ2667968.1"
                     /translation="MKVLLSGGGTGGHVYPAIAIANKIRDEHPDAEIIFVGTEKGIES
                     EIVPKYGFELKTVTVQGFKRKIDFDNVKRVFKLFKGLEQSRKIVKKFKPDIVIGTGGY
                     VSGPVLFNASMGKIPAIIHEQNSFPGVTNKILSKTVTKVLTSFEDSHKRFPEAAEDKL
                     VFTGNPVRKEILLSRKNIARKNLSISDEKRMVLCYGGSGGSRKINDAMRLVIKNMVNE
                     DIAFIFATGKSYYDEFMGSISDINLKPYQKVVPYLEDMANALAASDLVIGSAGAISLA
                     EITALGKPSIIIPKAYTAENHQEYNAKSIEKQGAGIAILEKNLTPESLNTAVFKLLGD
                     RELLVDMANASKTIGKPEAIDLIYDEIMKVYNSTQKSASKKTKKEKVIKEVKEIKKET
                     TPSIEGQAKVIGIKKR"
     gene            complement(31315..32445)
                     /gene="spoVE"
                     /locus_tag="ACOIAF_000948"
     CDS             complement(31315..32445)
                     /gene="spoVE"
                     /locus_tag="ACOIAF_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein E"
                     /protein_id="HGZ2667969.1"
                     /translation="MPKENLKKQIDIRMKTEFDGVVFYTTMLLVFVGIVMVFSASFIQ
                     SSFKHNDAYYFLKKNVIYAVLGFIVMIITSRIDYSFWKKNATAIGAIAVVLLLLVLTP
                     LGIEANGAKRWLGIGALTFQPAEIAKFATIILTAKLIEKNYDKIKSLTKGVVPLLVVP
                     GIFFALIILQPNLSTAGTVILVTFVMIFVAGMDMKIVFAMIGSGAALFAALVIAEPYR
                     LSRVTSFLDPFQDPLGKGYQVIQGLYALGSGGLFGLGLGKSKQKYFYIPEPQNDFIFA
                     IIGEELGLIGCIIVIMLFVVLVYRCVRIALKTSNVFACMVVIGIGAQIGIQAALNIAV
                     ATSSMPATGVALPFISYGGTSLTIFMGAVGIVLNISKHVKIN"
     gene            complement(32497..33852)
                     /gene="murD"
                     /locus_tag="ACOIAF_000949"
     CDS             complement(32497..33852)
                     /gene="murD"
                     /locus_tag="ACOIAF_000949"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HGZ2667970.1"
                     /translation="MDLKGKKVLLVGLAKTGISTIKHLDKLGASIIVNDIKDENKLRN
                     ILDELKSINDIKYILGHHPEDVDDIDMVVVSPGVPLDLPFILKLKNSGKYIIGEVELA
                     FKLSNNPIFIGITGTNGKTTTTSLVGEIFSRAKRDTYVVGNIGNPVIDTIETSSEESV
                     LVTELSSFQLESIDEFRPKVSAILNITEDHLNRHHTMEKYIEAKANIFMNQTVEDFCV
                     LNYDDEIVKSLADKCNAKVIYFSRTKKVNGGVYLENNDIIIDIDDKIKFLNKDDVSLP
                     GGHNLENCMAAIAIAYVCKIDLEVIKDVLMTFKGVEHRQEFVRNLDNVIYVNDSKGTN
                     PDSTIKAIQSYDRPIILIAGGMDKGSNFDELLETAKSYVKSLVLLGETASNIENCAKN
                     KGFNDIHIVKDMEEAVKTSYEISKSGDIVLLSPACASWDMYESFEVRGKDFKDNVNNL
                     K"
     gene            complement(33871..34839)
                     /gene="mraY"
                     /locus_tag="ACOIAF_000950"
     CDS             complement(33871..34839)
                     /gene="mraY"
                     /locus_tag="ACOIAF_000950"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HGZ2667971.1"
                     /translation="MMLGITELTYTALIAFLIVIIIGPIFIPMLRKFKFGQTVRDDGP
                     QTHLAKNGTPTMGGIIMIVAILITGLTRVKVSHDMAVGLICIAGFGFIGFLDDFIKIK
                     LKRSLGLKAYQKIILQVALSFYVAFYQYTSSSSASQLMIPFTDFVINVGILYIPIMMF
                     IIVAIVNAVNLTDGLDGLASGVTLIVSVFFMLFASSIAGNTEVAVLAAATVGACLGFL
                     GFNSYPARVFMGDTGSMALGGAVVAFSVLTNSVLIIPIIGGIYFAEALSVLIQVGYFK
                     ATRKRFFKMAPIHHHFEQCGWPETRVVFIFWIITVVLAWISIIAVF"
     gene            complement(34880..36253)
                     /gene="murF"
                     /locus_tag="ACOIAF_000951"
     CDS             complement(34880..36253)
                     /gene="murF"
                     /locus_tag="ACOIAF_000951"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HGZ2667972.1"
                     /translation="MECLTIKELVLATNGKLIYGDYNDCVSDIVIDSREANAQNAFVA
                     IVGENLDGHTFMKSAYDSGCKTFIKNESNGIKLESSDINLIEVKDTSLALGDISKYYK
                     EKFDIPFIGVTGSVGKTTTRDMIYSAISAKLNILKNEKNLNNHFGVPLTLFNLNKEHE
                     CAVIEMGMSGFNEIKYLVDIVNPKIAVISNIGLSHVEKLGSQEGILKAKMEITSNFDE
                     TNTLIVNGDDKFLSTLKEKEHAYKLKTFGFNKNNDVYCESYTMEEDSLTFICVINGKK
                     EEIFIPTIGEHNIYNAMSAILVGLCLNISLDYIKKGLKNFKGTKMRLDIIKNEKLTII
                     NDSYNASPDSMDAALKILGRYKGRRVAILGDILEMGEISEYGHRLVGKSSMNNTDIII
                     TIGENSVFIGEEAKQLGFNSANIYHFENREDVFNKLNELVKTGDTILVKGSRGMRLEK
                     IVEYLNK"
     gene            complement(36420..38399)
                     /locus_tag="ACOIAF_000952"
     CDS             complement(36420..38399)
                     /locus_tag="ACOIAF_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein D"
                     /protein_id="HGZ2667973.1"
                     /translation="MRKVKRISKKRLVLVLILACALFFCLVIRTGYLQLMKGNWLSTK
                     ALEQQTRDIPIEPKRGTIYDRNMKELAVSVTKYTVWCKPVEVEDKKEAAEKVAEILDE
                     DYKDIYALISKKNMALVKVKRWIDDDKASQIRDAKLSGIWVAEDNQRYYPYGNFAPYV
                     LGHTSSDATGISGVEMQYDKKLKGKPGKLIVSTDASGREIPQGMEKYYEPVQGNGLVL
                     SIDEVIQHYTEKAVQKAYELNNAKKVTAIAMNPKTGDILALASKPDYDPNDSRTPIYP
                     YYQEELEKYNDKDKIKGYYQMWRNPAVSDTYEPGSTFKLITSSSALEEGVIKDGEKFT
                     CTGSVTVGGRKIKCWRHYRPHGTQEFKQAVQNSCNPVFVELGSRLGVGKMYDYIEAFG
                     LMDKTGIDLPGEAKGILYNEKNVGPVELATISFGQSISVTPIQLITAISSIANGGDLM
                     QPRVVKSYTDNKGNITETVKPKKVRSVISKETSKKMLEIAESVVTEGGGKIAYIPGYR
                     LGGKTGTAQKVIDGKYAPGKYICSFVGIAPCDDPQIVVLAIVDEPTGVSAFGSTTAGP
                     IVKEIMNDSLKYLGVKPVYKEEEKAEYEKKQVKVPDVRNLKIGDAVKVLEDAKLKPDL
                     DADIELPEDTKVKDIFPKPGVKVNEDSSITLYFEN"
     gene            complement(38411..38758)
                     /locus_tag="ACOIAF_000953"
     CDS             complement(38411..38758)
                     /locus_tag="ACOIAF_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2667974.1"
                     /translation="MKKNYKIHNLNEYKNKKKNNYKKRNKKKIGAKCILLIATFSVII
                     LNLCGYSKISEMKYDIYYLQKDLRKKEIVLEELKAKETEKTSIKEIELKAKELLNMDY
                     PKDGQIKYIDVDN"
     gene            complement(39244..40179)
                     /gene="rsmH"
                     /locus_tag="ACOIAF_000954"
     CDS             complement(39244..40179)
                     /gene="rsmH"
                     /locus_tag="ACOIAF_000954"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HGZ2667975.1"
                     /translation="MEFHHVSVLLNECIENLNIKPDGVYVDCTMGGAGHSKEIVKKLS
                     DKGLFIGFDQDKNAISTAKERLSEYESRVKFVHSNFENIKEELEKIGVYKIDGVLADL
                     GVSSHQLDEADRGFSYMQDAPLDMRMDVRCEFSAYDVVNTYTEDELTKIIKDYGEDNW
                     AKRIAKFIVEERANKPIETTGELVDVIKKAIPKKARIDGPHPAKRTFQAIRIEVNNEL
                     GVITKMINDASSIMNEGGRICIITFHSLEDRIVKNAFKHLASDCICPQHLPICQCDKE
                     SEVKIITRKPILPSEEEIEVNPRSRSAKLRVAEKI"
     gene            complement(40209..40955)
                     /gene="lgt"
                     /locus_tag="ACOIAF_000955"
     CDS             complement(40209..40955)
                     /gene="lgt"
                     /locus_tag="ACOIAF_000955"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HGZ2667976.1"
                     /translation="MDRVAFTLFGIDIMWYGILMACGMILGTLIAIKEAKRVGIKEDD
                     VLNIAIIAIPVGLICARIYYVVFNWSYYAQNMSQIFNFRGGGLAIHGGLIGGILAGYI
                     YTKIKNINFLKMADTVILGMPLAQAIGRWGNFINGEAHGGATNLPWGIMVDGVKVHPT
                     FLYESIWDFGIFIVLLLFRKNKKYEGQVIVTYITLYSIGRFFIEGLRTDSLMLGPLRM
                     AQVISLIGVIGGIIAHVYLSKKNKNNISEE"
     gene            complement(41349..42635)
                     /locus_tag="ACOIAF_000956"
     CDS             complement(41349..42635)
                     /locus_tag="ACOIAF_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HGZ2667977.1"
                     /translation="MEKIVNDILKEEVYYEKLQNGLDVYFMPKRGFMKKYAILATNYG
                     SNDLEFVPIGEDKKIRVNEGIAHFLEHKMFEQPDGGDAFDKFSKLGVNANAFTNFTMT
                     AYLFSATENFYESLEHLIDYVQTPYFTDENVEKEKGIIAQEIKMYNDDPDWNVYFNCL
                     KAMYVNYPARIDIAGTVDSIYKITKEELYKCYNTFYNPGNMALFVVGDLDVEKVIDVT
                     KKSNNYKVDRLSKSIERFYPEEPESVKEKEVIEKFPISMPMFNIGFKDSNVGLKGKEL
                     LRKEIVTDILVGMLFKKGSKLYEDLYMQGLINENFGAGFSSQVDYAFSIIAGDSKEPK
                     KVKEIILDYIEKSKKEGLSKEEFERTKKKKIGSFIKCFDSINFIGNSFISYVFKDINL
                     LDYLDVIKDITFEEVEERLKEHFKEEYCVISIVEPK"
     gene            complement(42650..43915)
                     /locus_tag="ACOIAF_000957"
     CDS             complement(42650..43915)
                     /locus_tag="ACOIAF_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HGZ2667978.1"
                     /translation="MVNTKKIINLGNNINLTLIKTEKFKSNLISLYVQRLLDEKETTK
                     NALIPSIIASGSAKYPSARAISNKLDDLYGSSMGADAVKRGERQVLSFKVINISEKYL
                     DESIFEEVVEFFNEVINNTLVVDGGFKEEYLNIEKENLREKIQSIINDKKEYAQDKCI
                     EAMCKGERYSVSEFGYEDEIDSITSRELYEHYKNILKTSPIDIVVEGNFDEDKVVDII
                     SKNLKFEREEIINIPRADFIKNVDEVKVIDEQMEITQGKLVMGYRANVDYADIDKYYA
                     LVVGSNVLGGGPHSKLFVNVREKESLCYYIFSSIEKYKSIMFISSGIETKDYDKAVEL
                     IKKQVESVKAGDISDEELENSKLALVNSMKSITDNIGGMSDFVFSQSMAKTNSEVQDI
                     ISSIEKVTKKDIVEAIKNIELDTVYFLRN"
     gene            44231..45328
                     /gene="ychF"
                     /locus_tag="ACOIAF_000958"
     CDS             44231..45328
                     /gene="ychF"
                     /locus_tag="ACOIAF_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HGZ2667979.1"
                     /translation="MKLGIVGLPNVGKSTLFNAITQAGAESANYPFCTIDPNVGVVSV
                     PDERLNKLQELYNSEKIVPTAIEFCDIAGLVRGASKGEGLGNKFLSHIREVDAIVHVV
                     RCFEDENVVHVDGSVDPLRDIETINLELIFSDIEILERRINKTQKAAKADKTLGSELD
                     LLKSIMSTLEESKCVRTMEFTEDEQTFVNSLDLLTSKPVIYASNVSEDDLADNGENNK
                     YVQQVKAFAETEDAEVVVVCAQIEAEISELDSAEEKKEFLETLGLEQSGLDKLIKSSY
                     ALLGLISFLTAGPKEVRAWTIKVGSKAPQAGGKIHSDIERGFIRAETIAFNDLVEHGT
                     MAAAKEKGLVKLEGKEYIVKDGDVILFRFNV"
BASE COUNT        14150 a         8397 c         4745 g        18109 t
ORIGIN      
        1 tttttaaatg tccatgtatc gctttatttt tgccataatc ttaacaacta atttttaaag
       61 tttattatct taacaaagtt cattatctta tataatattt aaatgcatag acttctttaa
      121 tttaaccatc taaatattta attaaatatt attatatgta atataactaa tttttaaatg
      181 aatgtatagg agctggaatc cttcctcctc tttgagtaaa taattcagat gaatttgtat
      241 ttataggcat tactggtgct cgtcctaaaa gtcctccaaa ttctaccata tcacctacat
      301 cacatcctgg ggctggtata attctaacgg ctgtagtctt attattaatc atacctattg
      361 ctgcctcatc agctatcatg gctccaagcg tacctgctgt agtatttcct ggcacagcta
      421 tcatatcaag acctacagaa caaatagcag tcattgcttc aagcttatct atacttaaag
      481 cacctttaat aacagcatca atcatacctg catcttcaga tactggtata aacgctccac
      541 taagtcctcc aacatgtgaa catgccatta ctccaccttt tttaacagca tcatttaaaa
      601 gtgctaatgc tgctgtagtc ccatgagtac caacaacttc aagacccatt tcctctaata
      661 tatttgctac tgaatcacca atagcaggtg taggagctag tgataagtct attatcccaa
      721 atggaacatc caatctacta gaagcttcct ttgccacaag ttgtccaact cttgtaattt
      781 taaatgcagt ctttttaact gtttctgaaa caacatcaaa aggctctccc tttactttct
      841 ctaaagctct cttaacaaca ccagggccac ttacaccaac atttataatt ctatcagcct
      901 caccaacacc atggaaagct cctgccataa atggattatc ttctactgca ttacaaaata
      961 ctactaattt tgcacaacca aaaccttttg catcttttgt taattctgca gtttctttta
     1021 tgatttctcc catttctttc actgcatcca tatttatacc acatcttgta gaacctacat
     1081 ttactgaagc acatacttta ttagtacttg ctagagcttt tggaattgac ttaattagta
     1141 tcttatcacc ttttgtatat cccttttgaa ctaatgctga gaatccacca ataaaatcta
     1201 tacctaaatc ctctgcagcc ttatccaatg tctgtgcaaa ctttacatag tcattttcat
     1261 ctgttgcacc tgcaataatg gaaattggag ttactgatac ccttttattt acaattggga
     1321 taccaaattc acgctctata tcttttccaa ccttcaccaa atctttagca gaactcataa
     1381 tcttattgta tattttttct ctagctttat ctccatcagg gtctatacaa tctaataaag
     1441 aaatccccat agttatagtt cttatatcca actgttcttc atctatcatc tttattgttt
     1501 caagtatatt tcctacattc actattatca cctcccctta ctgtctacta cagattatgc
     1561 attgaattaa atatttcttc tcgctgaatt tttacatcca ctgataattt cttacctctt
     1621 tcaaccaagt cctcaaccgt tttctcaaaa gaatttaatg atttctctaa atcaactatc
     1681 ataatcatag taaaataatc ctgtaatatt ttttgtgata tatctactat gttgatattt
     1741 tgcttgtata attcatcact aattcctgcc actatacctg gtttatcctt tccaattact
     1801 gttaaaattg ctttcataat aatatcctcc cttatttttt tataaatctt atatttttac
     1861 aaattgaact atgtgttaac atatatttca tttatttgtt agtaaaccac tttttatgta
     1921 tatatttaat atattttaat acctatacat tcatttgtca attttttttc aatattctat
     1981 tatttttata ctatttagaa aattcatata ttatatatgt tttctcaaca acaaatacag
     2041 ttttcataat aatgaaaggt attacttagc aatctcatga ttttctaaat aatacctttc
     2101 taaaaattat ttttttaatt ttatgaattt aattatactt tttctagtat caggatttac
     2161 aattaatata gaaccaatca taattggtgt tagtatacaa aataaaaaaa taatatcttt
     2221 ttttagatta tattctttag attctaattt ataattatta tttattattc catcataatc
     2281 acttatattt gacttatttt tatttgtatc ttgatttttg ttagaaatac tttccctata
     2341 aggaaaagaa tgttcattgg taccaaatac tgattttgag cttaaatcat aattatcatc
     2401 attttctgta attgaatttt tattgtaaaa tcctttttct ttatcagaat tattttcttt
     2461 attaccatct ttaggtatat catgaggatt tttattttca ttgctatcag tacttgattc
     2521 tacatcatct ttaggagtat ttggttgttt ctcatcagaa ttatcatttt cattattatt
     2581 attttcatta ttattatttt cattattatt gttttgattg ttattactat cattattttc
     2641 atttccatta ttcccatctt gattgttatc atcctcatct ctatccgggt cgtctctacc
     2701 tcctcctcca cggtcaccat caatatcatc attttcccgt tcatcatctg gtgtctcttg
     2761 gttgtctccg cctcccatta taaatcctgg tttaaaaact atatttgaat acccttcttt
     2821 taatcctcct acaactttta ctctaaaaca acgcaactta aaatcttcaa aaaaaagtat
     2881 agcttgatct ctaatatcct cctgttcttc agatatccaa ttgattccat cgcttgaata
     2941 ttcaacaaaa accttacttg cactatacgg catatcataa aataaaacag ctacatatcc
     3001 attttttgta ttttgaaatt ccagttccaa attgtctatt ggttttttct ctataacaga
     3061 tacattcaaa ataggaacta catcatcatt tattataatt ttttctttat ttaacatttc
     3121 tgttttaaac tttcctgtta tattaaaatt ttctcccttt tccaactttt cataataatc
     3181 agtaaaatcc cattctacat ctatatcaag tcgattacca ctagatgttt ctaataccat
     3241 atatttggga aattttatat tttctatttt atcatcttta cttactttta tttcatcact
     3301 aaatacacca attcccctat gacaacattt cacaatatca tagccagaat cttcagctat
     3361 agagggtata agctcattat aatcatcaat tatataaact ttcttacttt ctgatataat
     3421 tgattgtgcc aacttattat tatctcttag taacccactt tcattgttgc tactatatgc
     3481 ttttattgaa gtttcttcta tctcaacatc acctttagaa taaacaccta tagcatgttc
     3541 tccattcact tcaatatcct tatttagtat tctaatatca ttttcagtat aaattcctat
     3601 tgcatctttt ccatttgcct ttattttttc aacagattcg taggatgatt taatatgtaa
     3661 gtctcctcct tcttcaacgt ataaggctgt cccatcagta gcaattatgt tgtttatacc
     3721 aatacttata cttccaccat tttctatatg tattactcct tcttttcctc cttcacctat
     3781 tatattcaat tcattatcat caataataaa tctcccatga ttttttatca atattttata
     3841 tttgttggta tctattgtaa aattactgtc ataaggaata taaaatctaa actcttcatc
     3901 tattaccata tcacaagaaa gagtataaac tatttcttct ttattatttt tattttcttg
     3961 aacccacctt tcaaaatctt caagacaatt ttcctcttgt ccaaaaactt tatgtggaaa
     4021 acttataaaa cacaaaacta ttatactcat aaaaataaat atctcatact ttttcatctt
     4081 acactcctag aaatttttta gctttgaaac aatcctgtcg aaacatatac ttcacccact
     4141 ataatttttc cataattatc aattattgtc aatttatata agtatcttta ttaattgtta
     4201 taaataaaaa gctacttact aaagtaaaca gacgtttcac tttaataagt agctatattt
     4261 ttataacaaa atagttataa tattagaact tccttcattt acaatttttt gtaaagtctt
     4321 ttgcatttta actcttatct cttctggcat agtatataat ttactttgta attgctcttt
     4381 tactaagtca tgtagtgact taccaaacat atttgattcc cataaatctg ctggttgctc
     4441 ttcaaatact tccataagat attttatcat ctcttcacct tgattttgat ttccaactat
     4501 tggagaaacc tctgtagata tatctgcttt tataatatga agtgatggtg cattggcttt
     4561 taacttgata ccatattgtt ttccttgttt cattatttct ggttcttcca gactaagttc
     4621 ttctaaggaa ggagctacta ctccatatcc ttttatcttg gcatctatta aagcactttc
     4681 tattttatca tactcatttt taactttaga tagtctagtt atcagactaa gcagttgata
     4741 atctccttca attttaaatc cacttttttc ttcaaggaca ttatagaata aatcttgctt
     4801 tgtctgtaaa tcaatattta taacaccttc tcctagctct acattatcaa cacctgtatc
     4861 ttccaaaaac tctaactcag aaaatccttg tataatacct tctatatccc ttattttacc
     4921 tatatctata atactttgtt ttaatgttgt tattatgcta ctttttatcc aatgatttct
     4981 ttcaagacct tcaacccatt ttggtaagtt gattcttatt tctgtaagtg gaaaatcata
     5041 taatacagct tccataacct cctctatatc ttcttcttcc atttcaacca cattcattgg
     5101 aagaactgga acttcatact tttcttccaa ttcacttcta agcatactcg tttcctcact
     5161 ttttggactt aacgtattta aaaccactgc aaatggtttt ttaagatttt tcaattcttg
     5221 tataactctc tcttctggtt ctacatagct ttttctgtca attccagtaa ctgaaccatc
     5281 tgtcaagaca actatcccta ttgtagaatg gtcttttata acttttttag ttcctatctc
     5341 tgctgccttt tcaaatgtca tagcttcttg tgaccaagga gtggatacta atctttgttt
     5401 tccaccttcc tcatgcccta aagctccttc aactatgtat ccaacacaat ctaccattct
     5461 tacttttaaa gatactgtgt cttttatttt tatttctaca ccatctgctg gtacaaattt
     5521 tggttctact gtcattatcg ttttacctga accactttgg ggtatctcat ctcttgttct
     5581 atccttttta aattcattgt ctatattagg tataaccaac ttttccataa attttcttat
     5641 aaaagttgat tttcctgttc ttacaggtcc aactactcca atatatatat ccccctgagt
     5701 ccttttggat atatcttcgt atatgttatt attcatatta ttcctcctat acatctaatc
     5761 ttattaaatt atatttagag ttaagggtta atattcttaa aatagaaaaa taaagaccag
     5821 caatattaat ttaatttaca tatagaaatt tgtaaatttc aatgtaaatt aaattaatat
     5881 tgctggtcca aatagtagtg tattatatta actgacttta gtagtatttt catttgaatt
     5941 attattcatc tcaataagtt ctttacgtct ttcttttgtc atgttcatcc atataaatgt
     6001 aaacaccaat ccaattatgg ctactatagt aagaactatg aagtagtacc tatatccagt
     6061 tacacccgga aatgcatcca atatcttccc tgctgctaaa ggacaaagta cttctggaag
     6121 atatcctaat gtagcaacta ttcctatagc agttcctgat attgataatg aataattacc
     6181 ttcttcaagt aacgaatagt gtaaaccttg aattgcatac atagatacat atattgcaac
     6241 acatccaatc aatagtatat aaatcataga tgattttgtt ggtgtaaata ttacacctaa
     6301 aagacctgca atcataagta taaatccata agatataatc tttgatgaac caactttatc
     6361 tccaagtaat ccagaagcag ctgatgctac tggtctacaa tactgagcta atattgagat
     6421 tgcagatgca aacaccacag tagctccaaa tgcttcagtt gaataaggcg taaaataata
     6481 aaatgacata ttagctgaat atgaacaaaa cattattatt gttattatcc atgtatgagg
     6541 catttttatt actgcaaaaa aggcttcttt atcaaatttt gctgatacct ctttttcatt
     6601 atcctttaat ttgaaaaata ctaatactcc aactattaca tttattgcct agtataatac
     6661 tataatagct gtaagaccag atttatcact tacttttgat gaaatatacc caaatagtat
     6721 caatactaga gatagatgaa ttgcattggt aattccacga ccactttcca taaatccaaa
     6781 agcttttcct tgttcatttt cagatgctaa cattctaaca gcttttacaa gagctggcca
     6841 aaaagtaagt attgttgtta ttccccataa ggcatggatt aaaaacacaa caatatatgg
     6901 aggataaagt gttaatacaa gaccagatat tcctgtaata attaaagaac ctgaaagcaa
     6961 ttttctggct gaaatacggt ctgctaaaaa accaccaaaa aggtatgata ccattgcaag
     7021 tagaccataa atacttccta agcttcccat ttgtgtattt gtaagattaa atgcttgtgc
     7081 aaaagtatca taataataat tctttaaata tggtaaagca tatataaatg ctcctgctac
     7141 aaagagtatt atcaagactt tgaaattttt ccaaatgctt ggtttattta cttcaactga
     7201 catacttcct ccttatgtaa atacctgtaa attttcatga taattaatta ttgcttattg
     7261 tattgtttat tcaatatttc ggaatattta ttaatatatt aggttaatct atcaataatt
     7321 tatggtgagt tatgagctaa aagtaaactc ttttagctca ccataataat tatattttta
     7381 tttatactaa cgtccagagt tattagatat ctttttttca tagttcttcc atagcattaa
     7441 acctgttatt ataacaaaaa gacctcctcc tatcatttca atctcataga ttatattatc
     7501 ttttaaagtc tccataactg gtgccgttga gaaaagtatg ccaactacag ttactacaag
     7561 taccattctg gcaatattga tacaaactga atcacttttt gcaattgaat atggtctttc
     7621 ttcatttggt cttgtctttc taagtctaat ataagatgta aaaagcaaga catatggaaa
     7681 taatgctgtt aatgctgtca tagtaacaag aacattgtat atattatcta ctgatggtaa
     7741 taagtttgtt cctacaagaa gtagactcac tattatagcc tgaactatta ctgcatttga
     7801 tggtatattg tatttatttg taacagtcaa ttgcttagga aatacacctt ctcttacatt
     7861 tccaaaaagc atctttactg gacctgctat ataaagtatt aatgctccta gtactgataa
     7921 agttattcca aatgctacta ttcttataaa ccaacttcct atacctaaat cttgtgcaac
     7981 tttagctaat gcatctagta tccctgtaga tgctgcaatt ttatctggag gaagtatcat
     8041 tgttattgct actgaaccta agatatagat tcctcctatt aaaacagcca tagttaatat
     8101 agctttagga aagtttttct tagcattatc catttcggtt atgaaattag cagtcgtttc
     8161 agctcctgat aatgcaaata ttatagcaga tatagaaact aatgaattgg catctatttt
     8221 tggtattata ttttggattg tatatatcga tgctgatgga gcttttttga gaataactac
     8281 agacataaat gccatcacta taagtaatat agcaggaatt gtagaaccta atgctcctgt
     8341 attcgtaaaa aattttccaa atgccatccc acgagtactt actaaggaca acagccaaaa
     8401 tattatgagt gaaagtgcta aaataaacat cttattgttt gataaatctg gatttccaag
     8461 catatatgca atatttatcg ctaaaaaagt caagaaagaa gaataccaaa atatctttga
     8521 tgtccaattt agccatgaca ccataaatcc ccacttctct ccataagctt gtttaaccca
     8581 ttctccaagt ccaccatctt ttggatatgt tgcagccaac tctgcacata taagtgatgc
     8641 aggaacaaag aatataaaag caaacaataa ccaaactggt atagaaccaa gacccaatcc
     8701 aaaactagag gcagtagatt tagctatcca tctaatccca aatagtgctg atatattcat
     8761 aaatactaaa tcccaaaatc caattttctt tttcactgta attcctccaa tttcaaattt
     8821 attcagactc atacacaatg tattgactta aatatcaagt ataagttctt caacacttct
     8881 tggataatta gttataattg tagcaccatt cttacctact attgctgtat ctgagtgtct
     8941 aaatcctcca actccaggta tatatatccc tggctccata gaaaatacca ttccttcttc
     9001 taatattaat tcattgtcaa atcttaaata tggctcttca tgttctgata aacctaaacc
     9061 atgaccaatt ctatgatttg aatataactc tagaccatac tttgcaacaa cagctcttgc
     9121 tgcttcatcc accattcttg ctggtatacc agcttttatt gtatctaatc cagcttgttg
     9181 agcctctact gcaatcttaa acacttcttt ttgtctttct gttggtttgc ctataataaa
     9241 agttctttca ttttctgctc tatagttttc ataccaaacc tgtctactat gtattactat
     9301 atctcctctt tgaagtattc tagtattgga atctaaatga ggttgtgctg ttctatcaat
     9361 ccctgaacaa gtccagtttg caaatcctat atatgtatca ggataatttt cagaaacata
     9421 ttttagtaaa gcattatccc ctgcaacgtc aaactccaat tcactcatcc caactctaac
     9481 attttctaaa gaccctttta ttcctatatc agataggtaa cctgctatct tcaaaaattc
     9541 tatttctttt gcatctttta catatctcat ttcaaatatt ttacttccaa catctttgat
     9601 agcaaatgac ttatcagtta tatactctga aaagcttgca gatactatat ctttttctat
     9661 tccaacagtt gttcctttag ggtatttatt taaaattata tctaagtaat gtttatgtga
     9721 tattccatgt tctttcattt ctggtatttc ataatacaca tatacattat caacttttgc
     9781 aacttcttta gcatgtaatt cttctaatcc aggtactatt aattctattt tatcttgagt
     9841 tactactgtt acaattggtc tagaataagt tattgcttta aatccactaa tataaaattg
     9901 attgtctggt tcaaatatta aagcaatttc aatgcttttt tcattcatgt actttctcaa
     9961 tctttctagt ctcatattat ttgacataat gcacctcttt cttaatattc agataattta
    10021 ttcaatacaa caaggatgaa tatattattt taaaattctt aatattttta gtacttgtcg
    10081 tatttgtatg taaattaatt ttatttgtga ttattttatc ataatcatga ttattttgtc
    10141 aatcatgatt ttattttaaa aaatttttta tattctagtg attttaaatt atctaaaata
    10201 taacgagtat acattgtatt ttgtaatatt attcttgatt tatggtaagt ttaatgataa
    10261 aatcacgatt atataaacta tattgattat ttgtaggtga ataaaatgac aaataaagaa
    10321 ttacaaaaga aaaggatatt gatgtatttc atagaagctg ctcaaaatat tatggaaaac
    10381 gaaggtatag aaaatattac acttcgtaag gttgcagata tggctggcta taatagttca
    10441 actctatata aatactttaa aaatttagac catttaatat catttgcatc tataaaatat
    10501 tttaaagatt ataacttgaa tatttcaaga tgtatagaaa atgtcaatga tgaatataag
    10561 aaatatataa ttatgtggaa attattttgt aagcattctt ttgataatgc tcaagctttt
    10621 taccaggtat tttttaactt gtctagtgat gaacttagct atataacaaa acaatattat
    10681 gatatgtttc cagaagattt aggtattcat gatagtgaca tatctttaat gattacagga
    10741 gaaagtataa aggaaagaaa taagatacta ttaaacaacc ttgtagatac tggatatata
    10801 ccagctaaag atttagacat aatcaatgat ataattattt ctttttatca aaatctatta
    10861 ttcttaaaaa agaacacagg aaaaaattct gatgatgatg aattaactat gaaaatgatt
    10921 tcttctattg aatacataat aaaacatgct aaataaaatt aaaatatggc tgtctcaaaa
    10981 tagagataag ccttttttat tgttgaaata ataaaaatat atttaatttc atataaaaaa
    11041 gagtgcctcg taaactaaaa agttcatttt gagacacccc taaaataaat ttattattta
    11101 ttatacaata gtttatatat catcacctaa aactacttct tccatttcat gctttttact
    11161 tctcatcatt agttctattc caacttcttt aggatttgaa cctttcttta ttacagaata
    11221 tatagcatta gttataggca tatctatatc taatttttca gaaacatcat gtgctacttc
    11281 agtagcagtt atgccttcta caaccatctt tacttctttt aaagtatctt ctaaactcat
    11341 acctttacct atgagtatac cagctcttct atttctacta tgcatacttg tacaagtaac
    11401 tattaaatct ccaatacctg aaagacctgc aaaggtagac atattagctc ccatagcaat
    11461 tccaagtctg ctcatttcac taatacctct tgtcataagg gcagcttttg cattatcacc
    11521 ataccctaat ccgtcacata tcccagctcc aaatgctata atatttttta atgctcctcc
    11581 aagttctact ccaactatgt ctggattcgt ataaactcta agtttagggc tcataaataa
    11641 atcttgtatc atctgtgcta tttttaaatc tttagaagca actactacgg tagtaggaat
    11701 atcccttgct acttcttcag catgagatgg cccagatagt attacatatg gattttgtgg
    11761 taattcttct tcacagactt gagataatct aagcccagtc cctttttcaa gacctttagc
    11821 tacattaaca ataacttgtc cttcttttat aaatggtttt attttttggc aagtacttct
    11881 tacagcctgt gatggaactg ctaaaactat caccttacaa tcttttatag ttttttctat
    11941 atcagtactt atagttatat tatttggaaa taatactcct ggtaaatagt ctgtattttc
    12001 tttagttctg tttatttttt tagcttgttc ctcttttcga gtccacatgc tcacatcatt
    12061 accttttttt gcaagcccta aagctaatgc acttccccag cttccagtac ctaatacaca
    12121 tactttctcc atgtttaaca ccacctgagt ataatatatt tactattctt tcttttgacc
    12181 aatttttctc tctgtcccat ttaataatcg ctttatattt gctctgtgat tatatattac
    12241 tgatgcagtt aagaacagag ctactattaa accagcttta ttttttacca taatcataaa
    12301 tattggtgaa catcctattc ctacaacaga acccaatgaa acatactttg ttatagctac
    12361 caccaatata aatactgcta gacacattag tgttattact ggatttacag ctagcattgc
    12421 ccctagtgaa gttgcaacac ctttgcctcc tctaaatcct aaaaccgctg gccaattatg
    12481 acctgctaca acacatataa ctgccagata tccagctaaa agagtatcta ttccaaccaa
    12541 tcttgctgca aattctgata ttaaaacagc cattacacct ttaagaacat cacctaaaaa
    12601 tgtcattgct cctgctcttt ttccaagtgt cctaagtaca tttgtcgaac ctgcattacc
    12661 agaaccttga gtccttatat caaccccagc aattcgcttt gcaactatgt atgaggtaga
    12721 tatatttcct aaaagatatg caactacagc aataattata taactaaata tctccactga
    12781 atttcccccc tttagttata ttttaatata tttatttttt atttttttgt ctatactcaa
    12841 actttatgga agttccttca aatccaaaat tttctcttat cttattctca aggtatcttt
    12901 gatatgagaa gtgagttaaa tccttatcat ttataaacaa tgtaatttta ggaggtctaa
    12961 tgtcagtttg agtcccatag taaattttta atcttctacc tttgtcagat ggtggttgat
    13021 ttaacattac agcttctcca attacttcat tcagtgctga agttgatatt ctcttagaat
    13081 gttcatttga cacatattca actgtatcta gtatcttgtt cattctttga ttagtttttg
    13141 cagaaacaaa tacgattgga gcatacatca taaatggaaa tttttctttt atatcttttg
    13201 tgtaattact catagtctta ttatctttct ctattaaatc ccatttattg attacaaata
    13261 tgcatccttt tccttcatca tgtgctatac ctgcaacctt tgtatcttgt tctgttacac
    13321 cttctaaggc atctattact atcaatacta catctgctct atctacagca ctcatagctc
    13381 ttattacact gtatttttct attgtctcat atattttact ttttcttcta agccctgctg
    13441 tatctattag aagaaatttc tgtccatttt tttcaaaata agtatcaatt gcgtctctag
    13501 tagttcctgc aattggactt acaattactc tttcttctcc aagtatctta ttgagtatag
    13561 aactttttcc tgcatttggt ttacctgtta tagctactct tattatatct tcttcatact
    13621 ctgtatctaa tcctgctgga aaattttcaa ctatctcatc taataaatct ccaaatccca
    13681 tactattggc tcctgaaact gcaaaaggcg ttccaaatcc caactcataa aaatcgtata
    13741 tattatcaaa ttgactttga ctatcaatct tattaaccac taagattact ggtttttttg
    13801 tctttctaag caattgtgca atttctttat cagcagctgt tattccagct ttaccatcaa
    13861 ctataaaaag tattacatgt gacatgtcca ttgcaagcat ggcttgattt ctcatttgag
    13921 ataatattat atcttcgcta tcaggttcaa ttcctcctgt atccactaat gtaaaatatt
    13981 tatctaacca ttctacttca gcaaatattc tatctcttgt aactccaggt gtattttcaa
    14041 ctattgatat cctttttcct gcaaatttat taaatattgt agacttacca acgtttggtc
    14101 tcccaactac agctacaact ggcctactta tactcatttt atttctcctt ccatctttta
    14161 ttttctattt taaaatttta tccacaaaat cttttccttc attcaaacaa gggataattt
    14221 ttattctcat aagttcttct aattgattta attcaatatt atctaagaaa atttcttcat
    14281 ctgcttttag catacttctt gttatataaa gagcttctcc taattcttta tcttcaagtt
    14341 gttcttttaa atccttagct gttaaaagac ctgatacagt tattgtctct ccaaaaaact
    14401 tgtttttgat ttcatataca tttacttgaa gatttttaaa cttgtccatc atagcatctg
    14461 ccatactctg tataaattca tatgcagaat gaccagtagc tattgatact ttcttctctc
    14521 tttttagaat attctcaggc aatatattta gatactcttc aatctctgtt tttaacttac
    14581 gtatcatacc tacaccatct tcaaactgta aaaaaccttc atattcttca tatccaggca
    14641 attcactatt agatagtata taaaattcat ctgataaaaa tgcaaatcga gtacctaatt
    14701 tttcaagata tttctgttgt atttgatgta tttgctctat agttttactt gcgctcttat
    14761 cattaaatat atttaattct actagatttt ctctatgctt tgtaatccca actggaacta
    14821 tcgcaacact gtttacatac ggataaagtt gagctagttc ttttattgtt ctatccaatt
    14881 cctttccatc atttatacct ggacataaaa caatttgaca gttcatttct atatgagcat
    14941 ctgccaatct cttcattata ccatataatt ttcctgcaaa tttattgctt atcatttttt
    15001 gtcttaactc tggattagtt gtatgcacag atatatttat aggacttatt ctatatttta
    15061 taatattatt tatatcttct tcactcatat ttgtaagtgt gacaaaattt ccttgtaaaa
    15121 aagataatct cgaatcatca tctttaaaat aaagagtttc tctcatacct tctggcaatt
    15181 ggtctataaa acaaaacata cacttatttc tacagctttt agcttggtca attatcggat
    15241 ttgtaaattc aacacccaaa tcttcatcat agtctttttc tatctcgtat atgtagactt
    15301 cgccatcttt tttttgtatt tctacatcta aatattcatc acttagtaag aatctatact
    15361 ctattatatc atgtattggc tgttcattca cagatataag taaatctcct acctctatac
    15421 ccatttcttc tgcaatacta tctttataaa ccttacttat aatattattt acttttttta
    15481 catttacttc caatattttc accactcttt tgtattcatc aatgcttatt ataatgtatt
    15541 ttaatatgca atttatttat ttattatatt ttatgatagc atttttaagc atttcatgtc
    15601 ataataacac attttaattc taatagtcaa tataagtaaa tagctataaa atagattaga
    15661 acaaataata ttcaaattta agtttcttaa aaatttaaat attatttgtt cttaaaaaaa
    15721 ttttataaat ttaatgtttt actataatta acgtcccatt tgtcatatca tgaaccattc
    15781 cacctacaca ctttgacatc aacaatgcct tattttgtaa gtctttctca tcatttgcat
    15841 aaaatattgg acaacctcca ccatttttca tattcttatc aagtgtaact atagctaggg
    15901 tatattcatt taaacctata tccattccca tcttatgcac ttcctttctt tcctatttta
    15961 taatttttta gtgataagtt ttttccttta gagcttgaaa gtattgggca tgactttacg
    16021 gcttccatta ctttttctac atttttatct actggtaaat aagcaatcat tatactttca
    16081 tcctctttat ttcttctagg taatggtacg aatgaaggtt ctccataatc tccatataaa
    16141 cctaaccttg aatatatatt gtatactatt gcctgtcttt gacctgaatc aaataatatt
    16201 cctgcattag cataatcttt atttttagga attatttgta ttcctacacc tctattaata
    16261 tatacttctc tatcactttt tagacctata tttgttattc cctttaaatc tcctaccttc
    16321 aatatagact catctacaaa actaatctca gcaggcatta catcagctat gtctccaata
    16381 ctttttctag taagaagttt ttttaatata aaggctaaca ctaatccaca aattgaactt
    16441 attaccaaac tcattccttc acttattttt gtttctgcaa gtattacata atacaatcct
    16501 attgttaaaa aagatgtaac tatacacata taatttctca cttcataggt tctagctatc
    16561 tcttctatga atgcagctcc tctctttaca agttgaactt cctctaagtt ttgcaatgta
    16621 tttcttctat ttgctctcac ttgtctaaat tgttctgctg ctagagataa aaatgttatg
    16681 gatgtataag aacgatttat aagagctgga accaataatg ctcctaatga ggaagcgacg
    16741 aatgctaata taagttgaga tagtaaaaca tttggttgcg ttggatactg tttttggtct
    16801 aaattcaaca ttataattct agatgttata ccaataataa cagcaacaaa aaatactcta
    16861 tccattaaat cactcctaaa atatttaatt tgaaatttta catgtaaatc tacaaagtct
    16921 ttctagagaa atcactctgc tttccttttg atgtttctat gataggtgta cttttcacta
    16981 catctatcat gacatctata tctttgagta ttggcatata tggtataacc acagtacttt
    17041 tttctaaatt ggtcttagat atagcaacaa tatcaacctc atctacatct ctatctatcc
    17101 ccatatgaat aaatagatta tgtattatag cttgtctttg ccctatatca tttactattc
    17161 caaatgcacc taaatctctt ggtataactt ctatagccag acattcattt ctatactttt
    17221 ctcttgtact ttctagacct atgttagtta ttattacacc atttaccatt aaaataggtc
    17281 cttcaaaact tatttttgca ggaactatat ctgctatatc tgctatactg ttccttctta
    17341 aaaatcttct aaaaatcaaa ccaacaatag ctcctgacac aatagctaag attgtgcaat
    17401 aaaaaaagct aaggccatac tttcttgcaa aaacgatata aactatcgaa gataccagtg
    17461 cagaaaaaag acttatataa cttctagatt catacgtaga tgctatttct tcaacgtaag
    17521 catctccttt tggtactaat tcttcattat cgatattctt tagtgttatt ctttcttgtt
    17581 ctgcaagacc ttgaaactgt tgtattgcta ctgcaaaaaa tgtcaaggct gcaaactcct
    17641 tatctataag tgcaggtaat gctatagcac ctaacgaagc tgaaagccca gatataatta
    17701 tctgttctaa ataatcttgt ggtcttgatg ggtattgttt gtctgtaact cttaaaacta
    17761 atcctctaca taatattcct acaactaatg caactataaa agagtgtcta aataaatttg
    17821 cattcaataa actttcttcc attttctcag ctccttctct tatatagttt gtgcaaaaaa
    17881 caaaaaaaat tcccttctat ttaaggaaat ttttttgtaa tattgtaact ataaattcgc
    17941 taccaatgcc aagttcactt tttactttta tacatccatt tagtgatttg actatatgct
    18001 ttgttatagc caatcctaaa cccgttcctc ctacatccct acttcttgct ttatctaccc
    18061 tataaaatcg ttcaaatatg cgttctacat cttcttttgg tatccctaca ccattgtcta
    18121 taactttaat tacgattttt tcttcatctt ttaacactac aacttgtaca atcccattat
    18181 cttgggtata ctttatagca ttatcaacta ggtttaagaa cacttgcttt atatagtctt
    18241 tattggatag tatacttatt gatttatcat cacatttata taaaacttct atattttttt
    18301 cactagctga atgttgagtt atttcatata cctctttaaa tacatcatat actacaacat
    18361 tttccataag aagagtatct ttattttcta caaatgataa cagcaatata tcatcaatca
    18421 acctttttaa tctatctgat tcgctttcta ttatttctaa gaatctattt ctagtattca
    18481 catctatatt ttcatttgac tttagtgttt caacaaaccc acttatagat gtaagtggtg
    18541 ttttaagttc atgacttaca tttgcaacaa aatcacttct catattttca agtttaactt
    18601 ttttagtaat atcttctata tttactatcg aacctattat tacgttgtta gtattctgaa
    18661 gataaactgg gtctactttt atcttatatg cgatatcatc tttagtagta atattagcag
    18721 tttcattatc tttagaacca ataaattgaa gtatttgttt taatatttct tcttctttta
    18781 ttacaacatt aatattatga ccttcaacta attgatttgg ttcactcttt atcatttctt
    18841 ttgcttcatc atttatcaga agtatatttc catttacatc tatagctaaa attccatgag
    18901 atatactctt taatatagac cttaactgta aatgtttata ctgtacttct tctaaggttt
    18961 tatccatatt ctttatcata tgattaaaat ttctagctaa ttcgccaagt tctcctttgg
    19021 ctgatatgtt aagcctagca tgatattcat tattacttac tttttttgag acgtatataa
    19081 actcttttaa atatttcctt aatgttattg tgtatcttac agctaatata acaatggcta
    19141 tggacaacat aacaataaaa aaatatatac tatttaaatc ttccacaaga taacactcct
    19201 aaaatataac ctttttaatc tattttgtat cctacacctc ttatagtctc tatatatttt
    19261 tccgatttat cttcatcttc tatttttttt cttaaatatc ttatatgaac atctactgtt
    19321 ctagtttctc catagtattc atacccccat attttatcca aaagataatt tcttgaaagt
    19381 acttttcctt tattttgaat taataatttt aacaattcaa attccttaag agttaactct
    19441 atcttttcag agcctttggt aacttcatgt tttgataaat ctagttttaa attacctgta
    19501 gtaagtatat tgttttcttc acctaagcta gtcaaattat atctccttaa aacagaacta
    19561 atcctagcta acaactcttt tatactaaat ggcttagtta tatagtcatc agcacctatt
    19621 tcaagccctt caaccttgtc attttccata tttttagcag taagcattat aacaggaata
    19681 ttttttaagt ccttatcact tcttattttt ctcaaaactt ctattccact tatatttggt
    19741 aacatccaat ctaaaagtat taaatctggt ttaatttcct tagcctttat aaatccgtca
    19801 aatccatcat atgaataact aacttcgtaa tttgacacct ccaaattgaa ttttaatagc
    19861 tcaactatat gcatttcatc gtctataaca agcactttag tattcattaa aaagctcctc
    19921 tctattttat ctgtagtacc gttcctattc gttctgatgc cttattatgt tttctataag
    19981 agtggaattt atctgctcta caactagtgc ataaatttaa atttattata ttttcttttt
    20041 taactccact acatctaagc atatactcat taattttcca taaatctaaa tagtaacttc
    20101 cctcttttat tatatagaac ttttcatcat tatttgtaag aattgtgtta aatttttcta
    20161 ctaaatcttc agacacttca taacaacatg gtccaattga tggcccaatt atacatacta
    20221 aatcttcagg aactgtttta tagcattcag acattaattt tattgtttta tgacctatat
    20281 tactaaaagt ccctctccaa cctgcatgag ccagtcctat ggctttattt ttagcatcta
    20341 taattgatat aggtacacag tctgctgtaa acagtaaaag tggtactttt tccaagtttg
    20401 ttataagtgc atctccatct cttcttttcc ctatagtatc tttattgact atattaacta
    20461 tatctgaatg tatttgagaa ttactagtta gattttcaaa tgtcaaatca cattctttac
    20521 atacttttaa catatcttct ctattttttg catctatatt cttacatgtt ataactagat
    20581 ttacagaatt tagtttttca tcaagttctt cacaattacc acttaaatta acatactctt
    20641 taatatcttg tacatttttt atatttatat aatctttcat catttgtctc ctttttctat
    20701 cagtatactt ttaagttcat ttacaaaagt atttatatct ttaaattgtc tatacacaga
    20761 agcaaatctt acataggcaa cctcatctaa ctctttcaat ttatccatta ccatctcacc
    20821 gatttttttg gtttcaattt cttctataaa acatttattt atttcatttt caatatgtac
    20881 aacaatactt tcaatctcgt caatagaaac aggtcttttt tcacatgctt ttagcaatcc
    20941 atattttatc ttttctctat caaaatattc tctactatta tcctttttaa tgaccattaa
    21001 aggagttgtt tctattttct catacgtagt aaacctcttt ttacatgatt cacactcacg
    21061 tcttcttctg atagatttta aatctgtatg tctagaatct attacctttg attctttata
    21121 attacaataa ggacattgca tctatattcc tcctttgttt aactatatct caaactcatc
    21181 taagcttaat tctgaaccta tatttactat tattgtatca caacctattt taagtatatc
    21241 attccaaaat ataacttctt catcccctcc tcttgaaaaa aaacttctac ttccatctcc
    21301 aaaaatggta aatgagacta ccttaccttc gtgaatatcc atgtctatgt ctattataaa
    21361 ccccatcctt tttccatttt ttacatttat aatttctttt tccattatat cagaaactct
    21421 aatcatattg tactcaccct ttctttaaaa ataaatatgt atcgaagtaa aaaaaaatga
    21481 cctatatagg tcatttaaat taaaataatg tttacagtcc atggattact tatacatatt
    21541 ttctcatatt ctttaaagca tttttttcaa ttcttgatac ttgtgcttga gatataccga
    21601 tttcatcagc aacttctatc tgagttcttc ctttgtaaaa ccttaaatct aataccaact
    21661 tttctctact atttaatttc ttaatagctt ctttaagtga tatttcttgc aaccaatttt
    21721 catcagtatc ttttttatct tgaacctgat ccataacgaa tattgcatct ccattgtctt
    21781 gatatactgg gtcaaacaga gatattgggt cttgtatggc atcaagtgcc attacaactg
    21841 attctacttc taattctaat tcctttgcta tttctgatac tgtaggttct ttagaatttg
    21901 ttctaattaa tctttctcta acttgtagtg ccttatatgc tatatctttt aatgacctac
    21961 ttactctaat tgggttattg tctcttagat accttctaat ttctcctata atcataggaa
    22021 cagcataagt tgaaaacctt acattttggc ttaaatcaaa gttatctatc gcttttataa
    22081 gaccaataca acctatttga aataaatcgt ctatattttc tcctctgttg ttaaactttt
    22141 ttattacact caagactaat cttaaattcc ctcttacaaa ctgttgtctg gcttcttcat
    22201 caccattttt tatttgtaat aaaagttcct tcatttgctt atttttaaga actggaagtt
    22261 ctgatgtatt aacaccacat atttcaacct tattaacttg catatatttt cagtcctctc
    22321 tcaaaagtat tcatctattg aaattattta catttccaat ttttttatac tttttaagaa
    22381 gaattaattt agcattatac aaattttttc atttcttttt gcaaccttga gattattttc
    22441 ttttcaagtc tagatatgta ggactgtgat attccaagca ttccagcaac ttctttttgt
    22501 gttttttcaa tacctttatc aataagacca aatcttaatt ccataatttg cttttctctg
    22561 tcacttaatc tgtcaagtgc cataaccaac aaatctctat ctatttcttc ttcaattatt
    22621 ttgtatattt catcattttc tgtacccaat acatcagata ataaaagttc gtttccatct
    22681 aaatctatat ttaaaggttc atcaaatgat acttctgttt tctttttatt attttttctt
    22741 aagtacatta atatttcatt ttctatacat ctcgaagcat aagttgccaa ttttatattt
    22801 ttattcagtt taaaagtatt aactgctttt atcaaaccta tagttcctat tgaaatcaaa
    22861 tcttctacat ctatacctgt attttcaaat tttctcgata tgtagactac taatctaaga
    22921 tttctttcta ttaaaattga ctttacactt tcatctgttt ctagcttttg taaaagctcc
    22981 atttcttctt ctggtttcaa tggaggagga agtatatttg cccctcccat atagtatatt
    23041 ccctttggct ttattatttt taatttaata cctaacatag taataaagct tattattctt
    23101 tctttcaatc gtaacattcc ttgtccccct taaattttca cctaaaatcc aggcaatata
    23161 tttggattca ttattgctcc atattctcta tcattaaaat ttgaaatacc aagtattata
    23221 tttcctatct tttgctcatc tacttctata tagtcagctt ttaagcctat tattatgctt
    23281 gtcttattac ttccagcatg tttataaggt attattctaa ctctacctga agttttatca
    23341 tctaaacttc ctataatttc ttgtgctttc ttcatatcta tatcttcata attaatattt
    23401 aatagttttt ctggaataaa tccttctaac acactagact taactattat tacatcactt
    23461 tgacttattg ggtcttttaa caaattacca ctatctacta aagccttaca gcaaaaactt
    23521 ttttctagta agtttatatt tatagtcttt gtaagctcct taatgtactt aagcattttc
    23581 aaatctttat aaatgtattt taataattca caactcacat aagtacatgc aattaagaaa
    23641 gatattttta agtgacttat tcctgtaaaa taaattataa aataagtgct tccagaaata
    23701 aaaatgttta ctagataaaa aactaacgct acatgaacat aatctttttt gctcttactt
    23761 ctaaatgaaa ttaaagttat tatactcatg attattattt taaatggtac agtaaatagt
    23821 atattcattg atggatacaa ataagcaaca gaataggcac ctcctaaact agcccccaat
    23881 aacttatttt ttttaggatt atatctttta gtaagaatag aagtacaact tataataata
    23941 taatttatta ctaggttttc tataacatag tactcaatat acaccatgta tcccccctta
    24001 aaacctttaa acatattata agatagtatg tacaaaattt tatgtcactt ttgatggtcg
    24061 aatccaaaac aaattctatt taaaaagaac aatgatatat gtcttatact ttatgtcgtc
    24121 aaaagatatt tttttaatgc caaacaaata gacttttttg tagaatccaa tatatctagg
    24181 tagaagttat tcgctctact tttggtatat ttacaaaaaa agactacata atttaataat
    24241 atatcaagta aatcttttac ttatataaaa tcaaattata tagtctgtaa attcaaatat
    24301 aaattattgt gctagtaatc catttgcata ttttgcagat tcatttagtg cttgttctgg
    24361 agttatgtct ccagctatca tcatttgaag ttgtttccat aaatattcat atatctctac
    24421 tccatgaggt ccaactgtca atggtctata catatgttta tctcttgtca ccatttcttc
    24481 aaatcttggg tctccttggt atttttcacc tttagcaact ggagctctag gtatttcttt
    24541 gtggaaagct tccattgatt ctttgcttag catatactca agaagtttaa atccaagttc
    24601 tttattttta catgctgaca ttaatgttaa atcatctact gaagcaaaac ttccaactgt
    24661 cttatcttct aatgatgtta catatcccca gtttaaatcc gggaatgatt tatcaaaagt
    24721 acttgtagca gcagaggata gcattatagt aaatgcagct tttcctggtc caaatacact
    24781 ttcaatcatt tcattactat cttttgataa agcatcttct ggaatatact ttttcatatc
    24841 atttataaag tttgcagctt tcaagccttc tgcattatta aatttaactg attttaaatc
    24901 atcattatat atatctccac cagcttgcca taaatacgga taccagttcc agtttaagct
    24961 tccaaatact ttagaacccc aaccttgtgc aagaccccac tggtcgattt ttccatctcc
    25021 atcagtatct tttgttgctt tttcacagat tcttttaaaa tcatcccatg tttttggagg
    25081 tttttctcct aatttttcta gtatatcttt attgtagtac aatacgcctg gattagcagc
    25141 ttctataggt aaaccataaa taccacccat catttttgca tcatctaagt atagataatt
    25201 atctgaagtt cctgattttt ctaaataagg tgttaaatct tctaccgcac ccatttctat
    25261 aaattgagga aacatctctg catacatgta tcctatatct ggaccttcac ctgcacttat
    25321 tgctgttgca tatttttctg cgtagttatc ccatggtact atctgaaatt caatttcaca
    25381 gttattttct ttttcaaatt tttcaaatat cggcttccat gcatctgcat catggtctgt
    25441 taatggtgca gaccatacaa ctaatttttc tttaccctct ttgcctttag aatcttcttt
    25501 ttttgaacat cctgcaagaa ttgatactga tagaatccct accataacta aagaaagaac
    25561 cttttttagt ttaatcatgc ttaatcccct ttcagacttt aatttgtctt tttcctcaaa
    25621 tgtttacatt taattcagat ttaatattaa caaataagat tactcttgtc taatcataaa
    25681 aaactatact aactcctata actatatttt ttttctatac ctattagatt taattcatac
    25741 taaaaactta ctttttaaat tttatcaata tttaaaaata tttttaagtc tagccacaaa
    25801 ctaactatct aaattgaaca aaataaaaag aagtctattg acttctttat aagcttacat
    25861 attatcttct tcttcttagg aatgtaggta tctccatgct ctcatcatca tcttcttcaa
    25921 ttatacttgc ctttttagga ggctctattt tttcctcagc aattacttct tcttcaattt
    25981 cttttacagc ttgttttact gttgtattta atgaagaacg tatgcttgat ttaggtttag
    26041 tatctaaatc taaggcacca ttttgtaatc cttcaaatcc tgtcgcgatt actgtaatca
    26101 ttatttcatc acctaaatct tcttttatag aagctccaaa tataacattt gcttctgggt
    26161 cacaggattc cataactaaa gttgatgcct cattaacttc aaataatcct aagttaggac
    26221 cacctgtaac attaagaagt actcctttag ctccttgtat tgatgtttct agaagtggag
    26281 attgaatagc ttgtcttgca gcatcaattg ctctagtttc accactagca ctacctatac
    26341 ccatatgagc aagtcccttg tctttcatta tagtagtaac atctgcaaag tccaagttta
    26401 ttaacccttc aactgctata aggtctgaaa ttgattgtat accttgtttt aatacatcat
    26461 ctgcaactgc aaaagcatct aacattgatg tatttttttg tactatctgt aaaagtctat
    26521 catttggtat cgttataaga gtatctactt ttgatttaag ttctgctatt ccaccttcag
    26581 cgttcttcat tcttatctta ccttcaaatg caaaaggttt ggtaactaca cctactgtaa
    26641 gtatccccat ttcttttgcc aagcctgcaa ccactggagc agctcctgta ccagtaccac
    26701 cgcccattcc ggctgttaca aagaccatat cagctccttg aagtaacttt actatttcat
    26761 ctttactctc ctcagcagct cttttcccta cttctggatt tgctcctgct ccaagtcctc
    26821 tagttaattt ttctcctatt tgaactttat attcagcttt tgatgtatat aaagcttgtt
    26881 tatctgtatt tactgatata aattcaacgc cttttagttg agcttctacc attctattga
    26941 cagcattgtt tccaccgcca cctacaccta ttactttaat ttgtgcacat tcttctaatt
    27001 ctacgtcaaa gtttagcatc ataaaccccc ttctctcatg attcaaagtt atcgtatgta
    27061 ttaattatat cattttcttc ttttattttg ttaaccttta agtaacatat tccacaaaaa
    27121 cgtaaatttt cctttttaaa tttcttactt atattgtcaa atattactct ttaagtataa
    27181 aataatttac gtatatatta aatgctcttt aatttttacg ttttttgttc ataatattac
    27241 taataatata tcgtctaatt atagataagt tattaaacaa tctcatacca aaaactaata
    27301 cagcaacata gtaaagagaa actcctaact tttctcctat ataagtcaat aatatagcca
    27361 atatagcatt acctatgaaa ccagtaataa aaattacatt atcatagtta tcttcaaaat
    27421 tggattttat cgctccaaaa atagaatcca ttattgcaag aatagctact gacatatata
    27481 tggaataatc catagaatat gtaaatggaa tatagtaacc tactactaca ccaaatgcta
    27541 atcctcctaa aacccataac ataaatcttc acctctttat tttactttga aacacaacta
    27601 cattacaatc ctttttatta atttttttta ctttttcttt atttttttgt atttttcgtt
    27661 ttattttgca aataaattca ctttatttcc attattttcc tgaactttta tattaattcc
    27721 atatatatcc ttaagagatt tagcatatga ttctggagag tttatagctg cactcaataa
    27781 atctatatct cctatagctt ttattataaa tggttgtcca tatgtagtat catttactgt
    27841 aatagtagct cctgaacatt ttatcttact tgttgctagt actctttctc catttataga
    27901 aattgctctt gctccagcct tttttaaatc atttaaaacc ctcaaaatat ctatatcatg
    27961 tataatcaaa tcgtttggat tctgtccgtc ttttaactct ctctcactat ccctcatagt
    28021 aatagttatg cctggccctg taacagttga ataaccactt aactctttaa cttcttcaag
    28081 ctctttttgc ataagtgttt ggatagattc atttccattc ttttttgctt ctttatattc
    28141 attcaaagtt tttttgttct ttttaagagt ttcttttaaa ttttttattt ctttttttgt
    28201 attttctacc tcactttcaa tactttcagt ttgacctaat gttatgtata ctctatttgg
    28261 gtctagtgat tttatataag ttgatatgaa aacgcctaat atgaatgctc ctaatataac
    28321 tattttcttt ttcatactat tatttcccct ctgctttgtt tatatcacta tgaccattta
    28381 tttctatatt gtcttctttg gtcactttta ctttggcatt ataatatgtt tctatttccc
    28441 agacaatacc atactttata ttaagtgccg aagccaatgt atcagaatca ccaattgctt
    28501 ttattataat tggttctttt atgacagtgt cattcatata aagcttgtct tcttttaaat
    28561 acatatatgt attagaaact attctctggt cgtttataga tatggcactt gcttgtgctg
    28621 aatttaattt atttatcatt gataataata aatcataatt atatagtata ctatcatttt
    28681 tatcagagtt tcctaagtct tttgtagaat ttatctcaat ttttatgcct gttccttcaa
    28741 catctgtata tccagctaat gtttcatatt tttctatttc tgacttaagt atgtcttgtt
    28801 ctccttcatt gtttttgcct ctataagtct ctatatcttt cttcacacta ttaacttctg
    28861 attgtagatt ctctttttct ttttttaaag atttaagttc ttctgtaagt tgctctcctc
    28921 ttttaaaagt tgtcatccct ctattttcta tattaataat tttaagttgt aaactaacaa
    28981 gaacacccaa tacaatactt cctatgatta tacttttatt tataaatctt tttttcaata
    29041 tatcacttcc ttacaattgt ctatccttct ggactataca ctgccaactt tccataatta
    29101 agatttatag tccctccatt agtattttta ttttgcaggt caatcagaat tttacttacc
    29161 ctagatatat tataatctaa gttatcatca ttagaaagta agatttttat attacttctt
    29221 gtaagcattt ctatattgct ttcttttttt aagtttacat aatttatttt tctataaatt
    29281 ccttcttctt ttaaataatt taaagtttta aatacattct tcttagtttt ataattttta
    29341 aattttatag atttattatc atcaatgcta taatctacat ctactatcaa gtctttttta
    29401 ctttcattag aacctcttag ttcttccaat aaatttcctt ttttatctat ataacaataa
    29461 ttatcttcat cttttaatac tgcaaatatt tctttttctt ttaaacttat aattatctta
    29521 ttgggaagtt ttctttttat ttccacattt tctatatatg gattttttat aagtttattt
    29581 ttcatatctt taaaattgta tgcaaaaata ttttcattta gatttacatt tagttctttc
    29641 attatatttg atttggtcac cctcttattt ccaattacat ctattttctt tacatcaaat
    29701 aaatcacttt ttacaattat acatatacaa aaagataaca taagtataaa aactaaaact
    29761 atcatgacat tattcgtatt taactttctt ctttttttca aatataatca acccctttgg
    29821 ttaataaata cacctttaga gcttgttttt cctacaaaaa aattataata tttaaagtca
    29881 aatttttgca ttttattaca taacatccaa ataaggtatt aaattttata aataaatcaa
    29941 tatttcatca aaataaacat agcatactac atactattta tatatttaaa tctgtataca
    30001 tacaaataaa cccctaattt ataataattt agaaaatcta atatattata aattaggggt
    30061 aaatatttac ttatcttttc tttattccta ttaccttagc ttgaccttct atacttggag
    30121 tcgtttcttt ttttatttct tttacttcct taataacttt ttcttttttt gtttttttag
    30181 aagctgattt ttgtgttgaa ttgtatactt tcattatctc atcatatata aggtctatgg
    30241 cttcaggctt acctatagtt ttacttgcat ttgccatatc aaccaaaagt tctctatctc
    30301 ctaaaagttt aaatactgct gtatttaggc tctctggagt tagatttttt tctaaaatag
    30361 caattcctgc cccttgtttt tctatactct tggcattata ctcttgatga ttttcagcag
    30421 tataagcttt aggtattatt atagatggct ttccaagagc cgttatctca gctaatgaaa
    30481 ttgctcctgc gctacctata accaaatcac ttgctgcaag tgcatttgcc atatcttcta
    30541 agtaaggcac aactttttgg tatggtttaa gatttatatc gctaatactt cccataaatt
    30601 catcataata gcttttgcct gtagcaaata taaaagctat atcttcattt accatattct
    30661 ttattacaag tctcattgca tcatttattt ttcttgaacc tccactacca ccatagcaaa
    30721 gtaccattct tttttcgtca cttattgata gattctttct tgcaatattt tttcttgata
    30781 ataaaatttc ttttctaact ggatttcctg taaaaactaa tttatcttct gctgcctctg
    30841 gaaatctttt atgcgaatct tcaaaacttg taagtacctt tgtaactgtt ttagataaaa
    30901 tcttattagt tacaccagga aaagaatttt gctcatgtat tatggctggt attttcccca
    30961 tagatgcatt aaataaaaca ggtccactaa catatccacc agttcctata actatatctg
    31021 gtttaaattt cttaacaatc tttcttgatt gctccaaacc tttaaaaagc ttaaaaactc
    31081 tttttacatt atcgaaatct atttttcgct taaaaccttg aactgttaca gtctttaatt
    31141 caaatccata cttaggaact atttctgact ctattccctt ttccgtccca acaaaaatta
    31201 tctcagcgtc tggatgctca tctcttattt tgtttgcaat agctatagct ggatacacgt
    31261 gccctccagt tccaccgcct gataataaaa ctttcatcaa atcatcatct cctgttaatt
    31321 aattttgaca tgctttgaga tgtttaacac tattcctaca gctcccataa agatggtgag
    31381 tgaagttcct ccataactta taaatggtag tgctacacct gtagctggca ttgatgatgt
    31441 cgcaactgct atatttaaag ctgcttgtat tccaatttgt gcacctatac ctataacaac
    31501 catacaagca aatacattag aagtcttcaa agctattctt acacatctgt agactaaaac
    31561 tacaaatagc ataataacta ttatacaacc aatcaatcct aactcttctc ctattatagc
    31621 aaatataaag tcattttgtg gctctggtat ataaaaatac ttttgtttac tctttcctaa
    31681 gcctagacca aacaatccac ctgaacctag tgcatataga ccttgtatta cctgataccc
    31741 cttaccaaga gggtcttgaa aagggtctaa aaatgatgta acacgactaa gtctataggg
    31801 ttcagcaatt actaatgcag caaataaagc agccccactc cctatcatag caaatacaat
    31861 tttcatatcc attccagcaa caaatatcat tacaaaagta actagaatta cagtacctgc
    31921 tgttgacaaa tttggctgaa gtattatcaa cgcaaaaaat atccctggaa ctacgagaag
    31981 tggtacaaca ccttttgtca aagattttat tttatcataa tttttttcaa ttaattttgc
    32041 agttaatatt attgtagcaa actttgcgat ttctgcaggt tgaaatgtta aagctcctat
    32101 tcccaaccat ctttttgctc cgtttgcttc tatccctaaa ggggtcaaaa ctaacaataa
    32161 taatactact gctatagctc caattgctgt agcatttttc ttccaaaaac tataatctat
    32221 tcttgatgta ataatcataa ctataaatcc aagtacagca tatattacat tcttttttag
    32281 aaaatagtac gcatcattgt gtttaaatga agattgtata aaacttgcac taaacaccat
    32341 aactattcct acaaatacta aaagcatagt agtgtaaaac actactccat caaattcagt
    32401 cttcatcctt atgtcaattt gttttttcaa attttcttta ggcatccaac taatctacct
    32461 ccattcttcc atttaagtaa gtttagaggt aagagattat tttaaattgt ttacattatc
    32521 tttgaagtct tttcctctta cttcaaaact ttcatacata tcccaacttg cacaagctgg
    32581 tgaaagaagc actatatctc cactttttga aatttcataa gaagtcttaa ctgcttcttc
    32641 catatctttt actatatgta tgtcattaaa tcctttattt ttagcacaat tttctatatt
    32701 tgatgcagtt tctcctaata agaccaatga tttaacatat gactttgcag tttcaagaag
    32761 ttcatcaaaa ttacttccct tatccatccc ccctgctata agtattatag gtctatcata
    32821 agactgtata gcttttatag tggaatctgg attagttccc tttgaatcat ttacatatat
    32881 tacattgtct aaatttctta caaactcttg tctgtgttcc actcctttaa atgtcataag
    32941 tacatccttt ataacttcta aatcaatttt gcagacatat gctattgcta tagctgccat
    33001 acaattttct aagttatgac ctccaggtaa acttacatca tctttattta gaaatttgat
    33061 tttatcatct atatctatta ttatgtcatt attttctaaa tatactcctc catttacttt
    33121 tttagttctt gaaaagtaaa ttactttggc attgcattta tcagctaaag attttactat
    33181 ctcatcatca tagtttaata cacagaaatc ctcaacagtt tggttcataa atatatttgc
    33241 ctttgcttct atgtattttt ccatagtatg gtgtctattt aagtggtcct ctgtaatatt
    33301 taatattgcg cttactttag gtctaaattc atctatactc tcaagctgaa aactgcttag
    33361 ttcagttact aacacagact cttcacttga agtttctata gtatctatta caggatttcc
    33421 tatattacca actacatatg tatctcgttt tgctcttgaa aaaatttctc ctaccaaact
    33481 agttgtagtt gtctttccat ttgtacctgt aatccctata aaaattggat tattcgatag
    33541 tttaaaagct aattctacct caccaattat atacttgcct gaatttttta atttcaatat
    33601 gaatggtagg tctagtggta cacctggaga cactacaacc atatctatat catctacatc
    33661 ttctgggtga tgtcctaata tgtattttat gtcatttata gatttcaatt catcaagtat
    33721 atttctcaat ttattttcat cttttatatc atttacgatt atacttgcac ccaatttatc
    33781 taaatgttta attgtagata taccagtttt tgcaagtcct actaataaaa ctttttttcc
    33841 ttttaagtcc atttttcatt ccccctagcc ctaaaatact gctattatac ttatccaagc
    33901 taaaacaact gttataatcc aaaaaataaa tacaactctt gtttctggcc aaccacactg
    33961 ttcaaagtgg tggtgtattg gagccatctt aaagaatctc tttcttgttg ccttaaaata
    34021 tccaacttga atcaaaactg atagagcctc cgcaaaataa attcctccaa ttataggtat
    34081 tataagcact gagttagtta gaactgaaaa tgctacaact gcacctccta atgccattga
    34141 acctgtgtcg cccataaaca ctctagccgg atatgaatta aatcctaaga aacctaaaca
    34201 tgctccaaca gttgctgctg ctaaaactgc gacttctgta tttccagcaa tagaacttgc
    34261 aaatagcata aagaatacag aaactattaa tgttactcct gatgctaatc catctaatcc
    34321 atcagtcaag tttacagcat ttactattgc tacgattata aacatcatta ttggtatata
    34381 taatattcca acatttatca caaaatctgt aaatggtatc ataagttgtg aagcacttga
    34441 tgatgatgta tactgataaa aagcaacata aaaagataat gcaacttgaa ggattatttt
    34501 ttgatatgct tttagtccaa gtgatctttt tagctttatt tttataaaat catctaaaaa
    34561 gccaataaaa ccaaaacctg caatacatat aagacctact gccatatcat gactaacttt
    34621 tactcttgta agtccagtta ttagaattgc aacaatcatt ataattccac ccatagtagg
    34681 tgttccgttt ttagctagat gtgtttgggg tccatcatct ctaactgtct gaccaaattt
    34741 aaattttcta agcattggta taaatattgg tcctattata attactatta aaaacgcaat
    34801 caatgcggtg tacgtaagct ctgttattcc taacatcata aaactcctct cccttgtcaa
    34861 ttaactcatt agtttaagct tatttattta gatattcgac tattttttct aatctcatgc
    34921 ctcttgagcc ttttactaaa atagtgtctc ctgtttttac taattcattt aacttgttaa
    34981 acacatcttc tctattctca aagtgatata tattggctga attgaatcct aattgttttg
    35041 cttcttctcc aataaacact gaattttcac ctatagttat tataatatca gtattattca
    35101 tagatgattt tccaactaat ctatgaccat actctgaaat ttcacccatt tctagtatat
    35161 ctccaagtat cgctaccctt ctacctttat atcttccaag tatctttaaa gcagcatcca
    35221 tagaatctgg actagcatta tatgaatcat taattattgt taatttttca ttttttatta
    35281 tgtctagtct catttttgtc cctttaaaat tttttaaacc tttttttata taatctaaag
    35341 aaatatttag acacaaacca actaatatag cagacattgc attatatata ttatgttctc
    35401 ctattgttgg tataaatatt tcctcttttt tcccatttat tacacatata aatgttaaac
    35461 tatcctcttc catagtataa ctttcacaat aaacatcatt gtttttatta aagccaaatg
    35521 tttttaactt atatgcatgt tctttttctt ttaatgtaga taaaaactta tcatctccat
    35581 ttacaattaa agtatttgtt tcatcaaagt ttgaagtaat ctccatcttt gctttcaaaa
    35641 taccctcttg tgaacctagt ttttctacat gtgataatcc tatattcgat atgactgcaa
    35701 ttttaggatt tactatatcc actaaatatt ttatttcatt gaatccagac atacccatct
    35761 ctattacagc acactcatgt tctttattta gattaaataa agtcaatgga actccaaaat
    35821 gattatttaa gtttttttca ttttttagta tatttaactt tgcagatatg gcagagtaaa
    35881 tcatatctct agtagtagtt tttcccacac tacctgtaac acctataaat ggaatatcaa
    35941 atttttcttt atagtatttt gaaatatctc ctagagccaa agatgtatct tttacctcta
    36001 ttaaatttat atccgaactt tctaatttta ttccattact ttcatttttt ataaaagttt
    36061 tacatccact atcatatgca gacttcataa atgtatgccc atctaaattt tctccaacta
    36121 tggctacaaa tgcattttgg gcatttgcct ccctgctatc tataactata tcacttacac
    36181 agtcattata atctccatat attaacttcc cattagttgc aagtactaac tcttttattg
    36241 ttaagcactc cattattaaa ctcctccttt aaaaccttag ccatattctc ttagcaacaa
    36301 aaggcaatgt ataccgttat atatacattg cctacgataa ctcttaactt tataattata
    36361 tactatattt ctgttttttt gaagatgttt atgggtatat gttaattatc tgttactttt
    36421 tagttttcaa aatataaggt tatacttgag tcttcattaa cttttactcc aggttttggg
    36481 aatatatcct ttacctttgt atcttctgga agttctatat ctgcatctaa gtctggtttt
    36541 aacttcgcat cctccaatac ctttacagca tctcctattt ttagatttct tacatctggc
    36601 actttaactt gcttcttttc atactcagct ttctcttctt ctttatatac tggtttaact
    36661 cccaaatatt ttaaagagtc attcattatt tctttcacta ttggtcctgc tgtagtacta
    36721 ccaaatgcac ttactcctgt tggttcgtca acaatagcaa gaactactat ttgtgggtca
    36781 tcacaaggtg ctattccaac aaatgaacat atatactttc ctggagcata cttaccatct
    36841 ataacttttt gagctgtacc tgttttacca ccaagtctat aacctggtat atatgctatc
    36901 tttcctccac cttcagttac aactgattct gcaatttcta acattttttt agatgtttct
    36961 ttagatataa cacttcttac tttttttggt tttactgttt cagttatatt tcctttattg
    37021 tcagtataag attttaccac tcttggttgc attaaatctc caccattagc tattgatgat
    37081 atagctgtta taagttgtat tggagttact gatatagatt gaccaaatga tattgtagcc
    37141 aattcaactg gtcctacatt tttttcgtta tacagtattc ccttagcttc tcctggtaaa
    37201 tctatacctg tcttatccat aagaccaaat gcttctatat aatcatacat ttttccaaca
    37261 cctaatctgc ttcctaactc aacaaatact gggttgcaag agttttgtac tgcttgttta
    37321 aattcttgtg ttccatgagg tctgtaatgt ctccaacatt taatctttct tcctccaaca
    37381 gttacactac ctgtacatgt gaatttttct ccatctttaa ttactccttc ttcaagagca
    37441 ctggaagatg taataagctt aaatgtagaa cctggttcat atgtatcact tactgctgga
    37501 tttctccaca tttgataata gccttttatt ttatctttat cattatattt ttcaagttct
    37561 tcttgataat atggatatat tggcgttctt gaatcattag gatcataatc tggttttgat
    37621 gctaaagcaa gtatatcacc tgttttaggg ttcatagcta ttgcagtaac cttttttgca
    37681 ttatttaact cataagcttt ctgtactgct ttttcagtat agtgttgaat tacttcatct
    37741 atagacaaca caagaccatt accttgtaca ggctcataat atttttccat tccttgtggt
    37801 atttctcttc ctgaagcatc agtacttact ataagttttc caggctttcc ttttaacttt
    37861 ttatcatatt gcatttctac accagatata cctgttgcat ctgaagaagt atgtcctaac
    37921 acatatggtg caaaattccc atatgggtaa tatctttgat tatcttctgc cacccatatt
    37981 ccacttaatt tagcatctct tatttgactt gccttatcat catctatcca tctttttact
    38041 tttacaagag ccatattttt tttgcttatt aaagcgtaaa tatctttata atcttcatcc
    38101 aaaatctcag ctaccttttc ggctgcttct tttttatctt ctacttccac aggtttacac
    38161 caaacagtat acttagttac acttactgct aactctttca tatttctatc atatatagtt
    38221 cctcttttgg gttctattgg tatatctctt gtttgttgtt ctagtgcttt tgtactcaac
    38281 caatttccct tcataagttg tagataccca gtcctaataa caaggcaaaa aaacaatgca
    38341 catgctagaa taagaacgag taccagcctt ttcttactta tcctctttac ttttctcaaa
    38401 tctattcccc ctagttatcc acatctatat actttatttg tccatctttt ggatagtcca
    38461 tattgagcaa ttccttagcc tttagctcaa tttcttttat tgatgttttt tcagtttctt
    38521 ttgcctttag ttcctctaat actatctctt tttttcttaa atctttttgt aaataataga
    38581 tatcgtactt catttccgag atttttgaat aaccacatag attcaatatt attactgaaa
    38641 aagttgcaat cagtaaaatg catttagctc ctattttttt cttatttctc tttttataat
    38701 tattcttttt tttattttta tattcattta gattatgtat tttataattt tttttcaaat
    38761 tctcaccccg atagtatata gtttaaattt tcctctatac tatatttgtt attaatcata
    38821 tttcttcctc taaaatttca tcgctaaatt aatacactat tacacaatct aattgtattt
    38881 ttttctatac acaacaaaaa cctataaatt cacttatact tttatatgaa tctataggtt
    38941 agatattaat ttctatttat ttttatcact tctagtattt ttagataatt tcttccataa
    39001 atataatatg taactaacta tctactctct gattcctaag ttctccatta tattacttaa
    39061 taccatctat atctatattt ttatatctaa attttcatta tatatctcaa taacaaagaa
    39121 catttcttat agtgttaatt gttttttaat tgctttacca gtgcaacaaa atatataata
    39181 ctttttaatt aattagaata atcttatttt atatataaga ttattcttta gccttataac
    39241 tacttatatt ttttctgcta ctcttagctt agcacttctt gaacgtggat ttacttctat
    39301 ttcttcctca ctaggtaata taggttttct agttattatt ttcacctcag attctttatc
    39361 acattggcat ataggtaaat gttgtggaca tatacaatct gatgctaaat gcttaaatgc
    39421 atttttcact attctatctt ctaatgaatg gaatgttatt atacatattc ttccaccttc
    39481 attcattata gaagaagcat catttatcat ctttgtaatt actccaagtt cattatttac
    39541 ttctattcgt attgcctgaa aagttctttt agctgggtga ggtccatcta ttctagcttt
    39601 cttagggata gcttttttaa ttacatctac taattctcct gtagtttcta ttggtttgtt
    39661 tgctctttct tcaactataa atttagctat acgcttagcc cagttatctt ctccataatc
    39721 ttttattatc ttagttaatt catcttctgt ataagtattt acaacatcat atgcagaaaa
    39781 ctcacaccta acatccattc tcatatcaag tggtgcatct tgcatataag aaaatcctct
    39841 atcagcttca tcaagttgat gagatgaaac tcctaaatca gctaatacac catcaatttt
    39901 gtaaactcct attttttcta attcctcttt tatattttca aaattgctgt gaacaaactt
    39961 cactctactt tcatattcac tcaatctctc ttttgctgtg cttatagcat ttttatcttg
    40021 gtcaaaccct ataaataatc ccttatctga cagttttttt actatttctt tagaatgtcc
    40081 tgctcctccc atagtacagt caacatacac gccatctggc tttatattta aattctctat
    40141 acactcattt aaaagaactg atacatgatg aaattccata gttacctccc ttaaattcac
    40201 tttttaaact attcttcact aatattattt ttatttttct ttgataaata aacatgtgca
    40261 attatgccac caataactcc aattagactt ataacttgtg ccattctaag tggtcctaac
    40321 attaaactat ctgttcttaa tccctctata aaaaatcttc ctattgaata taaagttata
    40381 taagtaacta taacttgacc ttcatatttt ttattttttc taaatagtaa taaaactata
    40441 aatattccaa aatcccatat agactcatat aaaaaagttg gatgcacctt tactccatca
    40501 accattattc cccaaggtaa attggtagca cctccatgtg cttctccatt aataaaattt
    40561 ccccatcttc caatagcttg tgctaaaggc attccaagta taactgtatc agccattttt
    40621 aagaagttaa tatttttaat cttagtgtat atataaccag ctaatattcc accaattaac
    40681 cctccatgta tagcaagtcc accacctcta aaattaaaaa tttgagacat attttgagca
    40741 tagtaactcc aattaaatac aacataatag attcttgcac agattagtcc aactggaatt
    40801 gctattatag ctatatttaa tacatcatct tctttaattc ccactctttt tgcttctttt
    40861 attgctatta aagttcctaa aatcattcca catgccataa gtattccata ccacatgatg
    40921 tctatgccaa acagtgtaaa tgctactcta tccataacgt cacctctttg tattaattga
    40981 aaatatttcc aaagcaatat gctataatta caattaattg taattatact aaattatgat
    41041 aacacattaa tgttaattct acaaaaaata ttaaaattta ttctacaact ccctacacta
    41101 tattatatct aattactata aaatttttta aactgaatct ctcatgacta aagttacaag
    41161 tgtacataga tattttttaa tcaaaaagat tgatgcatat atacatcaac ctcttctatt
    41221 ccttatctat agctcttaaa tttttacctc aatttatata aatatatttt ataaatttgt
    41281 tttaccaaaa taaacattta aaaacataaa ctaatactta attagttatt cttattatga
    41341 ttaagtttct atttaggctc tacaatagaa ataacacaat actcttcttt aaaatgctct
    41401 tttaatcttt cttcaacttc ctcaaaagta atatctttaa taacatctaa ataatccaat
    41461 agatttatat ccttaaaaac ataagatata aaactattac ctataaagtt tatagaatca
    41521 aagcacttaa taaatgaccc tatttttttc tttttagttc tctcaaactc ttcttttgat
    41581 aatccttctt tttttgattt ttctatataa tcaagaatta tttcttttac ttttttaggc
    41641 tctttagaat ctccagctat tatgctaaaa gcataatcca cttgagacga aaatccagct
    41701 ccaaaatttt catttattaa accttgcata tacaagtctt cataaagctt acttcctttt
    41761 ttaaataaca tccctacaag tatatcagtt acaatctctt tacgtaaaag ttctttccct
    41821 tttaaaccaa cattgctatc tttaaaacct atgttgaaca taggcataga tatagggaat
    41881 ttttctataa cctctttttc ttttacactt tctggttctt ctggataaaa tctttctata
    41941 ctcttagaga gtctgtctac tttgtaattg tttgactttt tagttacatc tataactttt
    42001 tctacatcta agtcaccaac aacaaataaa gccatatttc ctggattata gaaagtatta
    42061 taacatttat atagctcttc cttagttatc ttatatatac tatcaacagt acctgctata
    42121 tcaattctag caggataatt tacatacatt gccttcaagc aattaaagta tacattccaa
    42181 tcagggtcat cattgtacat ttttatctct tgtgctatta tacctttttc cttttcaaca
    42241 ttttcatctg taaaataagg tgtttgaaca tagtcaatta gatgttctaa actctcataa
    42301 aaattttcag tagcagaaaa taagtaggct gtcattgtaa aattagtaaa tgcatttgca
    42361 tttactccta attttgaaaa tttatcaaat gcatctcctc catcaggttg ctcaaacatc
    42421 ttatgctcta gaaaatgtgc aataccttca ttcactctaa ttttcttatc ttctccaatt
    42481 ggtacaaatt ctaagtcatt tgaaccataa ttagtagcca aaatagcata ctttttcata
    42541 aaaccccttt taggcataaa atatacatct aatccatttt gcaatttctc ataataaact
    42601 tcttctttca atatatcatt aactattttt tccatagaca tctcctccat taatttctca
    42661 agaagtatac agtatcaagc tctatatttt ttatagcttc aactatatct ttttttgtaa
    42721 ccttttctat actagatatt atatcttgta cttcagaatt tgtctttgcc atagattgag
    42781 agaaaacaaa atcagacata ccacctatat tatcagttat agatttcata gaattaacta
    42841 aagcaagttt actgttttct aattcttcat cagatatatc acctgctttt acactttcta
    42901 cttgtttttt tatcaattct acagctttat cataatcttt agtttctata ccagatgata
    42961 taaacatgat acttttatac ttctctattg aagagaatat atagtaacat aaactttctt
    43021 tttctcttac attaacaaat aatttagagt gtggaccacc acctaagaca ttgctcccaa
    43081 ctactaaagc ataatattta tctatgtctg cataatccac atttgctcta taacccataa
    43141 ctaacttacc ttgagttatt tccatttgtt catctataac ttttacctca tccacatttt
    43201 ttataaaatc tgctcttggt atatttataa tttcttctct ttcaaatttt agatttttac
    43261 ttattatatc tacaacttta tcttcatcaa aattaccttc tacaactata tctataggtg
    43321 atgttttaag tatgttttta taatgttcat ataactctct tgaagttata ctatctattt
    43381 catcttcata accaaattca cttacactat atctctctcc tttacacata gcctcaatac
    43441 atttatcttg cgcatattcc tttttatcat ttattattga ttgtatcttt tctcttaaat
    43501 tctctttttc aatatttaaa tattcttctt taaacccacc atctacaaca agcgtattat
    43561 ttattacttc attaaaaaat tctactactt cttcaaatat actctcatct aaatactttt
    43621 cacttatgtt aattacttta aaactcaata cttgacgttc tccacgtttt acagcatcag
    43681 ctcccataga tgaaccatat aaatcatcta atttattgga tattgctcta gcactaggat
    43741 atttagcgct tccacttgct attattgatg gtattagagc attttttgta gtttcttttt
    43801 catccaatag tctctgaaca tataaactta taagattcga tttaaacttt tctgttttta
    43861 tcaaagtaag attaatatta ttacctaaat ttattatttt tttagtatta accatatgta
    43921 cctcctattt ttatttaaac atcaatttta aaattgaaat ttatttatat tttaattaca
    43981 tatactcaca tataaaaacc ttctatttaa ttatatcctt tttattattt tctaaaagca
    44041 atatttttat ttaaattaaa tacaaatatt atttaaatca atatgcttta aagtctagtt
    44101 tacaagcttg atatatatga tgtataatca atatgtatgt ctataattaa aacaattttg
    44161 caatcctata gattgattgt ttaaagttat ataaagaata taaaaaagaa taagagagag
    44221 gacgattaat atgaaattag gtatagttgg tttaccaaat gtaggtaaaa gcacactatt
    44281 taacgcaata actcaagcag gtgcagaatc tgcaaattac cctttttgta caatagaccc
    44341 aaatgtggga gttgtatctg ttccagatga aagattaaac aaactacaag aattatataa
    44401 ttctgaaaaa atagttccta ctgctataga attttgtgat atagcaggtt tagttagagg
    44461 agcttctaaa ggagaaggtt taggaaataa attcttatca catataagag aagttgatgc
    44521 aatagttcat gttgttagat gttttgagga tgaaaatgtt gtacatgtag atggttctgt
    44581 agacccactt cgtgatatag aaactataaa cttagaatta attttctcag atatagagat
    44641 tcttgaaaga agaattaata aaactcaaaa agctgctaag gctgataaaa ctttaggttc
    44701 tgaacttgat ttattaaaat caataatgtc tactttagaa gaaagtaagt gtgtaagaac
    44761 tatggaattt acagaagatg aacaaacatt cgtaaattca ttggatttac ttacatctaa
    44821 gcctgtaata tatgcttcaa atgtatcaga agatgattta gctgataatg gagaaaataa
    44881 taaatatgtt caacaagtta aagcatttgc agaaacagaa gatgccgaag ttgttgtagt
    44941 atgtgcacaa atagaagcag aaatttctga acttgattct gctgaagaaa agaaagagtt
    45001 tttagaaaca ttaggtcttg aacaatcagg gcttgataag cttataaaat cttcttatgc
    45061 tttacttggt ttaatttcat tcttaactgc tggtccaaaa gaagttagag catggactat
    45121 aaaagttgga agtaaagctc ctcaggcagg aggaaaaata cattctgata tagaaagagg
    45181 atttatcaga gcagaaacaa tagcctttaa tgatttggtt gagcatggta ctatggccgc
    45241 tgctaaagaa aaagggttgg ttaaattaga aggtaaagaa tacatagtta aagatggaga
    45301 tgtaatatta tttagattta atgtttaaaa tgattaatcg tatacacttt gactattata
    45361 tataataact aaaaaactaa gattataata atacaatatt a
//
LOCUS       DBGJFF010000016        45141 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.016, whole genome shotgun sequence.
ACCESSION   DBGJFF010000016 DBGJFF010000000
VERSION     DBGJFF010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 45141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45141
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.016"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            88..924
                     /locus_tag="ACOIAF_000959"
     CDS             88..924
                     /locus_tag="ACOIAF_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HGZ2667980.1"
                     /translation="MNIKKIFNNNVVVSSLEDGTEIIVTGAGVGFKKKVGDLIDENLI
                     SKKYFVQDDQRDKYNQILNKTSIEYFKISEEIIEKANEVLNTQVNDSIILALTSHIEF
                     AVQREKQGIKLPNLILNETKQLYREEFEFGLWAIDEIEKRIGIKLPEDEAGYIAIHII
                     NGLENSQKDEGINILEFSKQVIQIIEEVHGFKLDVNSLNYTRLITHLKFFVQRILRKE
                     TYNDSDIEDMYKLVNKNYNKPKVCTEEIAALVLDRFEYKISKEEELYLMIHINKITNS
                     GC"
     gene            1277..2854
                     /locus_tag="ACOIAF_000960"
     CDS             1277..2854
                     /locus_tag="ACOIAF_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HGZ2667981.1"
                     /translation="MKKNKLGLWNFFQMLGKTFMFPIALLSVSGMMLGLGAGFTDPKM
                     IEMVPFLGNEYVNLVLNFMLTIGLFAFNNLGALFAMAIPLGLLKKEKEFGAFSGLVGF
                     IAMHIGTNFYLTRADLLVPAEQMSTNGQAMIMGIQTYNTSVLGGIVAGLIVYAIYDKV
                     SNLKIPESLGFYSGPRLVPIVSLIVMSIVGLLIPIVWPPFFNAFQSLGRWISSAGPLG
                     YFSYAVAERVTIPFGLNHLVTSVFRFTPIGGTAVIDGETYFGTLNMFMAYVENNQIIP
                     LDLAGKMEQGKLMIQYGLAGAALAIYRTAKPENRKALKGLLISGVLTVIIGGISEPIE
                     FLFLFISPALFAFHTFMNGLANMVLPYLGVLMGFTGDLIAFISFGVLRGTATGWPIAV
                     LVAVLYFAIYYFVFKWAIIKFNIKTPGREDKEIKVSEGNGSDFKNLSTYKGQQMISAL
                     GGQSNILSLDNCVTRLRLKLQDVSLINEDAIKEAGGIAVVKLDEHTIQVIIGTQVYSL
                     RKQMDKAMEGHLEACND"
     gene            2847..4031
                     /locus_tag="ACOIAF_000961"
     CDS             2847..4031
                     /locus_tag="ACOIAF_000961"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HGZ2667982.1"
                     /translation="MINFDKKVDRLGTYCTQWDYVEDRFGKKDLLPFTISDMDLEFPF
                     EITEVLQERLNHRVLGYSRWKHDDFRNSVTNWYIKRFNCSVDSNWIYYSPSVMYSISK
                     LIEMLSNEGDGILINTPAYNAFYDVIINNNRNIIKSHLVNRNGFYSIDFNDFEKKCKK
                     SKIFILCNPHNPTGRVWSELELVKMIGICKNNDVKIISDDIHMDIVYDNRMTPILNVA
                     KDYLDSVFICTSASKSFNIPALTGSYVIIPNEGIRNKFENITRYRDFVNSPAILAILA
                     TMVCYDDCEYWLDELLKYLKENLKYTIDYIKDNLEPLKLVMPEGCYFAWIDFSELKIS
                     GEKFQKLLMDIGEVAIMSGSVYGEEGEFYLRLNVACSREKLEDGLNRIKKTMDYIAKR
                     IL"
     gene            4049..4648
                     /locus_tag="ACOIAF_000962"
     CDS             4049..4648
                     /locus_tag="ACOIAF_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HGZ2667983.1"
                     /translation="MGYLKTMVDVMTTEMNAIRCLIDEAGIEYESIVSEIANCEGKVI
                     FMGVGKSAHIGKKLAATFASTGTPSFFVHATEAVHGDLGMIESKDITILISNSGNSME
                     VVNCIKYIKAIGSKTIAFTSNRNSVLAKECDYALIYPAKDEADHLNLAPTTSSTITLV
                     LGDSIACALSKSSNFGSSDFYKYHPGGSLGEKLKTANNG"
     gene            4672..5142
                     /locus_tag="ACOIAF_000963"
     CDS             4672..5142
                     /locus_tag="ACOIAF_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HGZ2667984.1"
                     /translation="MFKMFKKKSIKSPISGEVIELSKIQDGVFSQKIMGEGVAIDSTG
                     DIVYAPINGKIVVVAETKHAFVIELENGMELLIHVGLDTVNLKGEGFEVLVSMGEQVK
                     EGTPMLKINRPLIESKGISLITPVTITNHSEYNMNTCNVGNSVEGGKDTVIEFK"
     gene            5501..6247
                     /locus_tag="ACOIAF_000964"
     CDS             5501..6247
                     /locus_tag="ACOIAF_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HGZ2667985.1"
                     /translation="MKIENLSIDDIKRGYIFNDDLNCYSCIKCTKTYEVGEIYKIGNR
                     FFEASKAIELHMELEHSDYLEQLLNSDSKYNTLTENQKQLFQLFISDLPDKEIAKQVG
                     VSTSTIRHQKFIFREKAKQAKLYLALYESVFEDKSNKSSTIIPIHEHARMVDERYVVT
                     EEERKHILDTSFESMNPLKLKVFSSKEKKKVVILSKILEQFERGKKYSEKEVNQILES
                     IYDDFATIRRYLIEYGFMSRNKECTKYWLT"
     gene            6308..6982
                     /locus_tag="ACOIAF_000965"
     CDS             6308..6982
                     /locus_tag="ACOIAF_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HGZ2667986.1"
                     /translation="MNDELKSILYNKGVDIVRFVDISEFPINQTQGFSKAILFCIGLS
                     KKFITDIYNNLPTDSDEFLEKEEKVEELADWISKYIQNKGYRAYSQSEKNNLEHGYFE
                     KAYINPEMQSGISPLPHKTIANISGIGFMGKNNLFVTEEYGCAFSMCTVLTDAPISVE
                     RYPLIDSKCMDCNVCVENCPAKAIHGNEWTLPGKRESIIDVSKCFCVLKCMMSCPWSL
                     RYANQK"
     gene            7351..8697
                     /locus_tag="ACOIAF_000966"
     CDS             7351..8697
                     /locus_tag="ACOIAF_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HGZ2667987.1"
                     /translation="MEIETKTPDWINVLELNSKIDITGKYDVSNMIQNIVSDKSLEGS
                     IIYFPKGNYLFEKGIKISQQITLQGDSYYGGGNQVSNLDKKQPIIGTTNFITRGVSNM
                     SIITLSGTSQCIKNINFYYDSHDIEKIPPKNVSAITEYGETQGLSHFEHLFISGFSGI
                     GIEIPYYSTGNDITVSSCGLGMRLGEKSMLSSSKIYECKNGMEITTGVSLNNVRIEEI
                     KEIGINNKGFGFNLIMNLMVDQCGYCGFMFEKMYYSQITARITRCGQYYKSVDYNAYE
                     NMEDRKEEAYSIFYGDILENCNIVLMNSNIDTLESDSLNEHKIYVIKANETNNVMLTC
                     YAEADNFIQCAKGNLLLENGENTYKFYNGEICSVGGVQISDIDEKDLIKIIENTLYID
                     DKGKYLMVPRLKKNTVIYSVLDSTEEINKLHGGIWEQIDVKVLAGESMYYYKKIKD"
     gene            8724..10124
                     /locus_tag="ACOIAF_000967"
     CDS             8724..10124
                     /locus_tag="ACOIAF_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HGZ2667988.1"
                     /translation="MEESIIIKDISGWINVLDYGICNDETKDVSFKLQEIMDAVPEDS
                     IIYFPKGKYLFESGVKIKKRLTLCGDSYILANVPKNTAGVTQFTFKGKKNPKSENIAI
                     ITAKSVRHCIKNICFYSDSCDRDMTSNHEPPTDGKPRFHHNVIINYRNISAVICEDKQ
                     GEPGHYENLSFSGFSGIALDMPNNSTANDITIFTSGLGISTGENSTIANSKVWGCVDG
                     MIISTGTFINGMRVEEIKKVGIKNIGRGLNFITNITIDQCGYCGVWFDSISNVQISGN
                     ITRCGQYYYDVSYDNYLMLDNRVKEAYSLFYGNLLESSNIVLSNTNEDNWEDFKEDKH
                     KVYLIEALKTKNVLLTCNIDATDWIVKSEKGNLTYNNMRQTVIFYDGKLSSINGIGIS
                     DEDYGDKVKIKKGNIYINSNDKDIKMHKPEVGDIMSTIIKDKSILSAHYGGTWEKIGE
                     KIEFGITVHYYKLIEK"
     gene            10334..11770
                     /locus_tag="ACOIAF_000968"
     CDS             10334..11770
                     /locus_tag="ACOIAF_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HGZ2667989.1"
                     /translation="MEGRTGIKDGRWINVVERGIKNDGSYNIGPALQDIIDEIETDFC
                     VLYFPKGKYLFTSGVKINKEIVLQGDSNSSTGTTQFITRGVENMSIITLTGKKQCIKN
                     INFYSDSCEIQVNKEPPTKGNPKYHYEMIINKNEKNVEMNNVSAILYDNSKNMIKGLG
                     HYENIYISGFSGTGIRIPYYSIVNDITVSSCGLGIDTGIDTIISNSRISKCQNGMRIR
                     TGTSINNVRIEKIQKVGLISSQVKGYEGYGSYKINNITIDQCGYCGFSFDSMKDSYFS
                     GIIRRCGQYYYNTDYDTYLSIKDRVEEAYSLFYGNYFTECNIDLVSDNKDNWDDGLEY
                     KHKVYVFKTLQIPKLTLRCNIDAYDYIVKSVHGGGNLMLKNNFNNYIFIDSSNPEVIN
                     GINFNYQKNGNLLRLNNGKLSYKNGKEETAIFRPNYGLIVASTDSECEQIETSYGGKW
                     EKLGSEVIGEETVYYYKHHEPDKTNQIQ"
     gene            12114..13058
                     /locus_tag="ACOIAF_000969"
     CDS             12114..13058
                     /locus_tag="ACOIAF_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HGZ2667990.1"
                     /translation="MIVSVSRRTDIPAFYSKWFFNRLEEGFVYVINPMNPKQVSKIEL
                     NPHTVDCFVFWTKDVTPMLYDLNKLKDYKYYFHYTITSYGKEVETCILDKRKVIDSFK
                     ELSRKIGKERVILRYDPIFLSEKYTVDYHIKAFASLCNQLDGFTEKCIISFIDLYKKT
                     KFNTKALHIKPIEITEIETLSKEISKIAHKHGITLEMCSEEYNLSKFGIGKSKCIDDR
                     LISKIIGCEVEVKKDSTQRDICGCVKSVDIGQYNTCRHYCLYCYANFNYAKVEENCRL
                     YKEDNKLLIDTLRDDAKISIRDMKTIKVDNNKQISIFD"
     gene            13227..14159
                     /locus_tag="ACOIAF_000970"
     CDS             13227..14159
                     /locus_tag="ACOIAF_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HGZ2667991.1"
                     /translation="MNLLKKYPIPIASLILSIFTLGNLLQSYSESLRNVIGFIGFILY
                     AIYIVKIVLLRKGVKEQLENPLISSTFPTITMATMIFATYIKPLSLELSIMIWSIGIV
                     GHLVLIVLFSKKFLVKFSIKTVFPSWYIVYVGIVAASVTAPAFNQLLIGKIAFWIGFV
                     SYIVLIPVILKRVWIIKEMPEPSLPSIAILAAPGSLLLSGYINSFSDKNNVVLYLLFI
                     LSIVFYIIVLGYLPKLLRLPFYPSFAAFTFPMAISAMGMKLMNGYLTKSGHFISWISY
                     ASKLEEIIATVIILYVLVLFIIYLSKSETTSKAK"
     gene            14264..14731
                     /locus_tag="ACOIAF_000971"
     CDS             14264..14731
                     /locus_tag="ACOIAF_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HGZ2667992.1"
                     /translation="MKTKEEYLKEVRNISEEYFRKGEFFCSEAVLQTINDALGQPLSP
                     EITKLASGFPIGLGKAQCLCGAISGGEMALGIVYGRVHGEAMNPKMFEHAKGLHDYIK
                     KEYGATCCRAITKKWDGDNFMSPERKEHCIKITGQVTEWVADKLIEDEKLNIK"
     gene            complement(14976..15830)
                     /locus_tag="ACOIAF_000972"
     CDS             complement(14976..15830)
                     /locus_tag="ACOIAF_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HGZ2667993.1"
                     /translation="MLTIIIALLPVMGWGLMPIVANLKKSSPHEQLLGTSISAFIFST
                     IITLVIHPEITFFSFCISMISGIFWSLGQLMQFKAIQIASVSKVMPISNGSQLTFTTL
                     LAVILFNEWTSSKMLLIGSLSILCIILGILLTNYQTKKSTDKNMLKCVGVVLLSSLFL
                     SLYVVTNQIFLIEGYRIILPQSVGMLITSSIIVKYFSKEIVYRENVLFNLITGILWSI
                     ANLGMFITTNTLGVTISFSISQSCVIVATLGGIIFFKEKKNKKEWLAIFIGIILIMSG
                     VFMLSIIK"
     gene            15996..17960
                     /locus_tag="ACOIAF_000973"
     CDS             15996..17960
                     /locus_tag="ACOIAF_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HGZ2667994.1"
                     /translation="MLTDMIQNREIKIIKFLLKEGHTTVKAIADDLDVSEKTVSKSLK
                     EVEAVLKKIGLSLIRKPKIGVYIDGDLKKILPYLDNKGSQIPTTREERVIYIYSVLLK
                     SNDYITIQQLSDELYISRGTIERDLAEVEKLLKNEGISIYKKPSKGIKLNISERDKRQ
                     LTAKFIHKFWSRNWYIKQEQESFYQAFEKIQADVNGIFSEDDLSIIIDILRNFSRERK
                     FEFSDYEFQSLVIHLAIAVERIRKGEYIEESLYIGNIENNQMSNTNYLAKQLEEKFSI
                     LLPKSEIGYINLHLVASDQHRDNNELKNTSTNPCETSTNNISNLREIIKNTLYETAYD
                     KDLIDGLTSHVHSSINRLKYGLSIKNPYVNTIKQNFSLSFEQALDLKKVIEFIYDIRI
                     NDDECAYIALHFEAFRERMKELPQNIRVILVCSTGLGSSRLLSARIKKYFPMIKIEKV
                     LSVQELVSSKIDADLVISTIYLELETIPTIVVSPILNESDIRTLERNIQNVSSCTSKK
                     YKSFRNLIFEENILLNIEASNYEDAINHITDNLIKKGFAKEGIAKSAIERENLSYTSF
                     QSIATPHANPSYIKKSNISVATLKNPITWGNEKVEVIFFISLENDKNLELDEIYEVFY
                     DFIDNKKNINDILSATSTKEVYDLLVEESI"
     gene            17960..18421
                     /locus_tag="ACOIAF_000974"
     CDS             17960..18421
                     /locus_tag="ACOIAF_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGZ2667995.1"
                     /translation="MKISDVLKKEQIVFNLDTNTKEETIKKLSSIFVENGIVDNEEEY
                     VKSVLEREEHSTTGIGNGIAIPHGKSNAVKKSSIIFAKTKHKIEWDALDEKPVDSIFL
                     LAISNIDKDSNHLVLLSKLATKLMDDDNVDALKLANSEQEIINIFSEEGEM"
     gene            18424..18744
                     /locus_tag="ACOIAF_000975"
     CDS             18424..18744
                     /locus_tag="ACOIAF_000975"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HGZ2667996.1"
                     /translation="MKRKIIAVTACATGVAHTYMAAQALKKASKKMGHLIKVETQGAT
                     GIDNELTTKDAQIAEVVIFAVDTKVRNAERFEGKEILEVPVNAPIKDAEKVINDALKL
                     IDTK"
     gene            18760..19860
                     /locus_tag="ACOIAF_000976"
     CDS             18760..19860
                     /locus_tag="ACOIAF_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HGZ2667997.1"
                     /translation="MLKKIVSELKKHVLTGISYMIPLVIAGAMIMAISRVGGSFYNIP
                     DIWDAKYAESASSIVRLLHDLDGFGGTALGLMFPVIAAFIGFSIADKLAIVPGLVGGM
                     IAKDIGAGFLGALAVGLIAGYTCLFIKNHVKLPKSAASIVPIFIVPVFGTLITVVLIN
                     YVIGIPFATLNTGLENWLNGLSGTNQILMAAIIGAMMAVDLGGPVNKAALTTSLALLT
                     SGIYAPNTAAMVGIVIPPLGLGLATILAKQKYNKQLREAGKSSLIMGLIGVSEGAIPF
                     AVESPLKVIPSCVIGTAIASAMAVGLGSVNATPISGFYGWFTVENWPMYVLSIAVGTV
                     IVAGLVIVLRGNPVIEDDEFEDDDFDEAEWES"
     gene            19980..22580
                     /locus_tag="ACOIAF_000977"
     CDS             19980..22580
                     /locus_tag="ACOIAF_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HGZ2667998.1"
                     /translation="MIKAHIVNHTHWDREWYFTSGDALVLSEQLFTDVIDELERNPDV
                     SFVLDGQLSILDDYVQLHKEKIEIIKKLIKEDRLHIGPWFTQTDAFFARGESILRNLM
                     IGIFESKKYGKYMKIGYLPDTFGFNAQMPILLKHVGLDNIFFWRGIHLGKQVQSPYFK
                     WKSLNGESYIYAVNMPHGYGTGMLLEPSLKYVNGRLDPAIDFIKSYTDVDEVLIPSGN
                     DQLNIIGDFKNKIKAINDIGKYSYITSSYQEFLKYIKSIESLESYRGEFREPVLARIH
                     KSIGSSRMDIKLACDKLENKLIKRIEPLLVIAKKSKIEISNQLLINTWKKLLEGQAHD
                     SLAGCVTDTVTDDILHRIREANEICDSIENTIVKKISEGLKLSKNDILVFNTEAKRFN
                     GYKEIQVVSDSKNIYFKDNLDATIIEETYVKPRENVLEETPAGNIFIEEPGYYILKVR
                     INIQLPALGYKVVSFELSDKEMVSLDKSNDTFISNDNYKIIYEDGSLNLQLKDGTYIS
                     NFLTLKDSGNAGDTYDFSPLKNDEDIKLSFDKVYTEKALGYEKMVVKGTTLLPLTLED
                     RKNKYLNGKLNVKVSIVLSKENPLMDVKLCVDNTIYNHRLRVHIKTDIKDNKNIASLP
                     FGYITRKNGVLDNWEDIYSEMPIDLEPLESNITLTNQNRSCTIFTRGIKEYQHIEDEI
                     ALTLLATTDELGKPDLLYRPGRASGDTTKKGHIRIKTDKAQMLKKLEFSFAIYMDSKS
                     FDELEIANLTHNYLRECVNYQLQDYNFFLYRIDNKIQKSIVNKTVSRELEIISLPENY
                     LISACYPSYYSKDKFIIRVENPSSNRMILDEQIFKNRNGKIVNAIEDVEEEQVYEIAP
                     FDVISILLDL"
     gene            23049..23384
                     /locus_tag="ACOIAF_000978"
     CDS             23049..23384
                     /locus_tag="ACOIAF_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HGZ2667999.1"
                     /translation="MYNIDDMIYNCPVEALSNILGKKWVAIIIWKIQEDKKRFGELQR
                     AIPDCSKKMLVQQLDFLIEQGIIVNQKKLVNNVVESTYFLSESGLRLLPVMEKMIMWS
                     NKNLNCDKY"
     gene            23521..24798
                     /locus_tag="ACOIAF_000979"
     CDS             23521..24798
                     /locus_tag="ACOIAF_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein/redoxin"
                     /protein_id="HGZ2668000.1"
                     /translation="MQNVNLFLVFIEGIVSFFSPCILPILPIYLSILSNSSVENLKEG
                     KTSFIGSSLFKNTIFFALGISTTFFILGSSVKVLSMFFNENKDLIMFIGGIIIIIMGL
                     FYMGIIKSSILNREKRFNVKFKEMKAITAFILGFTFSFGWTPCIGPILASVLVMVSSS
                     SNHLSANLLIAVYTIGFILPFIITAMFYSKLFKTIDKIKSNMEIIKKIGGIILIVSGI
                     LMMVNGFGSISKHFNTSQNSKIESKQEENKRENSTDKEENSDGNDSQKDSNNDNNDKG
                     SNDEDRIKSIDFTLTDQYGKTHKLSDYEGKVVFLNFWATWCPPCKEEMPYIEQLYKDY
                     NKNNDDVVILGVASPNLGREGSREHVVNFLKDQGYTFPVVLDEDGALAYQYGINAFPT
                     TFIIDKEGYVTQYIPGAMDKATMASFIENQRNK"
     gene            24919..26307
                     /locus_tag="ACOIAF_000980"
     CDS             24919..26307
                     /locus_tag="ACOIAF_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HGZ2668001.1"
                     /translation="MKKTFDAIIIGFGKGGKTLAGDLANRGLKVALIEKSNKMYGGTC
                     VNVACIPTKSLENSANSVKTKNINSWDEVQAEYEKAIDKKETLITKLREANYNKLNSN
                     ENVTIFTGMGTFIDEKTVQVKTENEIYELVADNIFINTGSRPFIPNIKGIENKNIVYD
                     SESLMNLRVLPKKMTIIGAGFIGLEFAGIYSSFGAEVTILNSNNGILPNEDVEDSEEI
                     IKLLAKRNVKIVNNANIKEIKEVSELAIVEYEVDGKSKELTSNMILVATGRKANTEGL
                     GLENAGIELNERGFIKVSETLKTNKEHIWAIGDINGGPQFTYISLDDYRIVINQLFGD
                     KTRTTNDRKNIPNSIFISPAFSRVGLNVKQAKEKGYEVLVAKMPVEAIPRAKQIGKAD
                     GFIKIVIDKKSNKILGASMICENSSEIIHLIQLAVDLEVEYTYLRDRVYAHPTMTEAL
                     NDILSPNMIKEV"
     gene            26753..27391
                     /locus_tag="ACOIAF_000981"
     CDS             26753..27391
                     /locus_tag="ACOIAF_000981"
                     /inference="COORDINATES: protein motif:HMM:NF037435.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF434 domain-containing protein"
                     /protein_id="HGZ2668002.1"
                     /translation="MKAKEEIEWLLNRDYKIDPVVTFVGDRYQFSIRQRDALKRAVCT
                     DEKNIIRQSKRLSLDKIKEDTVNIDGFNLIISIEVALSGGTLVIGSDGNIRDLAGLRG
                     TYKIIDKTEEALNLIGKFFNKYNAQNLKFYLDAPVSNSGNLKYRILEHAKTWGIETEV
                     ELVKNADVVLEKLDRVVSSDAVIVDKCISYFNVARGIIEEYIKECNIINLNK"
     gene            27509..28378
                     /locus_tag="ACOIAF_000982"
     CDS             27509..28378
                     /locus_tag="ACOIAF_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HGZ2668003.1"
                     /translation="MRGWICVKLTKLNIKKYRAPIYKYALANVNLRSAKSTNSSIITV
                     IPQGAKMEVLDEEDDWIKVMYNSQEGYVYKDLVSVSEYAWSNLNLREDKSTTSNIITV
                     IPEKSRVEVLQVDGDWSKVVYDDKIGYVFNYFLSIDGNKPNELDYKYFYTDMIKFVNE
                     NNIKSTSDYLIVTDLRNKYTYIFKKDNGGWGQLYKWQCTIGKPETPTITGIFYISGRK
                     PSFGTDEYSVKYATRIKGGYYYHSVLYDSTGSYIIDGRLGEALSHGCIRLSTENAKWI
                     YDNIPDTTTVIIH"
     gene            complement(28533..29678)
                     /locus_tag="ACOIAF_000983"
     CDS             complement(28533..29678)
                     /locus_tag="ACOIAF_000983"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HGZ2668004.1"
                     /translation="MSIYYVPPINLLGKGCLNEAKDSIKSLGTKKAFIVSDKFLVSNG
                     TVKKVTDILSQIDIDFVVYDEVKQNPTVTNVNKGLKILKSENCDFVITIGGGSPQDCG
                     KAISILATNGGDIRDYEGINKTSKKCLPIIAITTTAGTSAEVTINYVITDEDRHVKMI
                     MVDTNSLATMTVNDPELMISKPSNLTAATGMDALTHAIEAVVANGAYDVTDSTALYAI
                     KQIFKYLPRAVKNGNDIEAREQMCYACFLNGIAFSNAGLGNVHAMAHQLGGLYDLPHG
                     VCNAMLLPIVEEENAKSAPAKFRPIAEVIGMDVNNKSDKECVDFVIDKIKALSEEVGI
                     PKSLKDVGVDNPNFELLAENSMKDACAGANPVFFDKDKIIELFMKIS"
     gene            complement(30011..30787)
                     /locus_tag="ACOIAF_000984"
     CDS             complement(30011..30787)
                     /locus_tag="ACOIAF_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HGZ2668005.1"
                     /translation="MTSDNHRPTARILDILEALAESENGYTLTEIADVINAPKSSIFP
                     IVHTLNARKYITIDKNTSKYSIGIRSYTLGSSFFEKRTIFNYIIDEMTNIVNSCSETC
                     QLGILDNNEVLYIGKVDSPEPIRLISYVGKKIPANCTGLGKALLCDFDKEKLISLYPD
                     GLKGFTEKSITDFDVLYDQLLEVQRTQIASECEESMEHLKCLAVPIRKNGCIRAAVSV
                     TLPLFRANDEKIELIKKLLLNAQKNIQSMMEERNEDIVIN"
     gene            31369..32343
                     /locus_tag="ACOIAF_000985"
     CDS             31369..32343
                     /locus_tag="ACOIAF_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HGZ2668006.1"
                     /translation="MIMDFIKKIPAGMMIVPMFIAAFINTFCPDIVQIGSFTTAVFSS
                     VGSATIIGVQLVCLGTQLQFREMPKVLKRGGILLGSKFAIGALIGILIGKLFGMDGVL
                     GLTTLAVISAVTNSNGSIYLSLMTSYGDETDCATMSLLTLNDGPFFTLIALGTSGLAN
                     VPLLSLLAAIVPILVGMIIGNFDKKMKEFLEPGCNLLVPFVGFALGSSINLASILKGG
                     LSGVLLGLISVFVGGIFIVFCDKFIGKRPGYAGWAVATTAGNAIAVPAAVALVDPSWQ
                     PYVATATTQVAAAVVVTAILVPLITSWWAKKYGCPKFPKQNEDGTITA"
     gene            32441..32746
                     /locus_tag="ACOIAF_000986"
     CDS             32441..32746
                     /locus_tag="ACOIAF_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HGZ2668007.1"
                     /translation="MINAVIIDEKDNVAVAIEAIAKGSEISFKLKDKTVKTITALDDI
                     TIYHKLAIKDMAKGDKVTKYGEHIGEANQEIKSGQHVHIHNVRSVREDLDKQVMTNI"
     gene            32902..34062
                     /locus_tag="ACOIAF_000987"
     CDS             32902..34062
                     /locus_tag="ACOIAF_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HGZ2668008.1"
                     /translation="MKFYGYKRPDGRVGIRNHILILPTSVCASDTTRIIASQVQGAVT
                     FNNQNGCSQVHSDQQLTMDVMAGYAANPNVYGVIVVSLGCENCQNDLVVDAIKERTNK
                     PIKTLVIQEEHGTLKTIEKAVRYAREMAQEASLLRKEEFPISELILGTECGGSDPTSG
                     LAANPLIGELSDKLVDLGATSILSETTEFIGAEHILARRAVNEEVKEKILHIVHRYEN
                     SLKLVGEEVREGNPSPGNIAGGLTSLEEKSLGCIHKGGHRQISEVYDYAKQIDKKGLV
                     IMDTPGNDASSVAGMVAGGAQIVVFSTGRGTPSGNPIAPVIKITGNKITFANMEDNMD
                     FDASPVIYGPQTMEELTDDLLNMVVDVANGKQSKAESLGYTEMAIARVCNYV"
     gene            34112..35014
                     /locus_tag="ACOIAF_000988"
     CDS             34112..35014
                     /locus_tag="ACOIAF_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HGZ2668009.1"
                     /translation="MKFVSFTEGENECKRTGVFSKDELFIIDVNSLNLSRNFKDLNEL
                     IQNVSSEDIIKLKTIINESTFYNYDVFKRERCTVHVPIEKPIHDILCVGVNYKDHLEE
                     TQTHFDDSFEEPQKTVYFTKRVTKAIGPEDEIDGHFEINNQLDYEVELGVVIGKTGVN
                     IRKEEVEDYIFGYTVINDISARKLQKEHVQWFRGKGLDTFTSLGPCILHKSAVPFPIK
                     LKVCSRVNGEERQSSNTGLFISDVSDIVSELSKGMTLEAGDIIATGTPSGVGMGFSPP
                     RYMSSGDVVECEIESIGILKNYIK"
     gene            35094..35987
                     /locus_tag="ACOIAF_000989"
     CDS             35094..35987
                     /locus_tag="ACOIAF_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HGZ2668010.1"
                     /translation="MKAEFLTPVVTIFDEEGNLDKEGNKKVYDHLIDGGVDGLVIMGS
                     TGEFFNMSMDMQKELIDLVTSHVNKRVKVFIGTSRMCINESIELSNYAHNSGADGVMI
                     ISPYYFSLDDDSIELFYSEIAKNTEAEIYLYNYPDRTGYDLSPELTLRLARKHKNIVG
                     YKDTVTLMGHTRDLINTMKIEFPEFKVYSGYDENFIHNLMSGGAGCIGGLSNLIPEVC
                     SKWAEAYNNRDMEKVIEIQKYINKAMDFYLITNPFIPAMKKAMNIRGLQISDYMTAPF
                     VKPNEKQVCEIKKLMNELNIC"
     regulatory      36040..36221
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            36449..37579
                     /locus_tag="ACOIAF_000990"
     CDS             36449..37579
                     /locus_tag="ACOIAF_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGZ2668011.1"
                     /translation="MKFKSNVLKQFFLIAMVIFMVGGLIGCSKSDKENKQASTGKKLE
                     LKYASGFSVEDLEDGIKKVTDGDNRELILIPKKIKIPEKYKNANIVRTPVDNVLIAST
                     TQACSLRTIDELDSIKAVTTEEQYWTIKEIKDLMKDGKIHYVGSNLAPDYEKIVDLNP
                     DLTIVSSGLSEADTKFIDKLKELGLNYVVDNEYKEQNPFGRMEWTSLIAAFYDKEDMA
                     TSELNKTVQKVEEVSKKIKNKAKPKVVWASVYEGSAYIAPKDSYVDNMIKMAGGINAC
                     ASIDSNEGRVSIEQLHEVAKDADIFVYSSSSDYAPNLDSIKSSAPVLADLDVVKNGNL
                     WVFAPDYYQSVDKTDELIVDLVEMFHPGTTGEKIKHYINYDK"
     gene            37651..38676
                     /locus_tag="ACOIAF_000991"
     CDS             37651..38676
                     /locus_tag="ACOIAF_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HGZ2668012.1"
                     /translation="MDFINKMRIRNTTIFIVLIISMILTFLVCVAFGSVKIEFKEVID
                     VVLRGMDNTEHYKIIYNIRIPRVLATMVGGACLAVSGILLQIFFKNPIVEPYILGVSS
                     GSTLSVALMILGGYTLGFESVNSMSIFVAAFIGALIVMSIVILFANKVKNITTLLIIG
                     IMTGYVCSGITNVLQSFANSDKLKEFTMWSMGSFSGFTWEKVEILIIVGILGLLMTVF
                     IIKPLNAFLLGEEYAKSIGINIRFFRVLIVFISSILAAIVSAFAGPIGFIGLAVPQIS
                     KMIFKTSDNRVLIPVVIFLGSIVTALCDLISRVILSPVELPISTITSLLGAPIVVMLL
                     VKRKDRA"
     gene            38679..39875
                     /locus_tag="ACOIAF_000992"
     CDS             38679..39875
                     /locus_tag="ACOIAF_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2668013.1"
                     /translation="MLKTNNLSVGYNNKVVISNINVEVKNGEILCLLGSNGAGKTTLL
                     RSLSKLISPIKGEIYLNGVNINCISRKALSKKMALVLTNRLLGDLMTVQDIVNIGRYP
                     YTGFFGSLSKKDLIMVDEALESVDALHLKKRYFDELSDGEKQKVLVARALVQEPEIII
                     LDEPTTHLDIKHRLELINILKKLSKEKSISVILSLHEIDIALKSCDKVALIKNNKVIA
                     YGQPEDVVDEDIINSLYELDDKNFNSLLGSVEISNKSKNEVFIIGGGGKATPIYRAFT
                     KKGIGLYSGIIHENDIDYEIGRTMGIKMFTENPFEPISDESFDLAIRNLNDSKIIIDT
                     GFSVGETNKRNIDIIKEALKLDKKVYSFRNRDESKKYYDSLDSKIEHIDKVSQIINSA
                     DINNLL"
     gene            complement(40003..40728)
                     /locus_tag="ACOIAF_000993"
     CDS             complement(40003..40728)
                     /locus_tag="ACOIAF_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HGZ2668014.1"
                     /translation="MKTISKQELEKTLEKHYLWLKNNNAGEKADLKELRLENMDLRGY
                     CLSNIDFSWSDLINLNLEKADLENSIFNNSYLSDCSLKNSILKNADLSGASFRFCDLS
                     NSDIRGANLENSNLEHATLNGVISDESTRFFKLYCPETGAFIGYKKCFNFRMVQLLIP
                     SDAKRVSATSNACRCDKAKVLSITSIDGKESFKSARAYADENFIYRVGETIVAEDFNE
                     NRWKESTTGIHFFLTREEAIGYL"
     gene            41206..43305
                     /gene="nrdE"
                     /locus_tag="ACOIAF_000994"
     CDS             41206..43305
                     /gene="nrdE"
                     /locus_tag="ACOIAF_000994"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HGZ2668015.1"
                     /translation="MNWIELNNQVIIKNEQGKYQLEKDKEALSSYIEAFIQPKLRKFN
                     NLEERLKYLIDEGYYSKEVINQYSIDVIKNIYDMIDKHKFEFQSYMSANKFYQNYALK
                     SNDGKEILETYNDKVLIVALTLGNGDKDLALNLADKIVKQEFQPATPTFLNAGRKRAG
                     EMVSCFLLSVEDSTEGISYAISSSNHLSKIGGGVALNLSKLRASGESIKDIEGAAGGV
                     VGVAKMLEQSFSYFNQMGARQGSGAVYLTVFHPDFELLMDTKKINADEKIRLATLSLG
                     AIIPDKFMELAEKNEVAYAFYPHTVYKKYGVSLDEIEMDEWYDKLVNDSDIRKKEINP
                     RQMLTKIAQMQQESGYPYVVYIDTANREHTLKDVGIIKMSNLCCEIFQYQTPSEIEGY
                     GGKNEWGQDISCNLGSLNIANVMDNKTIESTVETAIRALSFVADSTDIKPVPTVSNSN
                     SKSHSIGLGAMNLHGYLVRENILYTSDDAIDFSNVFFAMVRYYSIKASMKIAIERNQT
                     FEGFDKSEYVKGRNSKVLSKYYEQSYLPKSEKVRALFEGIYIPTKEDWTKLLDEVKEK
                     GIYNAYLMAVAPTQSISYVQNATSSIMPITEPVEVRTYGDSTTIYPMPFLTNDNMLYY
                     QSAYRMDMRKVIDLVATVQNHVDQGISTTLFVTDEKTTRDIARHYIYAYKKGLKSLYY
                     TRTKMTRDTHECLVCSV"
     gene            43318..44289
                     /gene="nrdF"
                     /locus_tag="ACOIAF_000995"
     CDS             43318..44289
                     /gene="nrdF"
                     /locus_tag="ACOIAF_000995"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HGZ2668016.1"
                     /translation="MTFNLHKIHNAVNWNREEDGFTQAFWEQNVKQFWLPEEISVSKD
                     IKVWNELSNKEKELYKKVLGGLTLLDTKQGNNGIPSMMSLTENLQRKAVLSFMGTMEE
                     IHAKSYSSIFMTLLSNLEIDELFEWIETEPTLQRKADLVLAQYENTTNQEGLYLSMVT
                     SVFLESFLFYSGFFYPLYLSGQGKMVASGEIISLILRDESLHGKYIGLLAQEIYDSFD
                     KMDKEMLEEKMYSILYSLMENEIEYTNVIYRESGLEKEVVNFLKYNANRALENLGFEK
                     LYTVDAINPIVLNGLSTETKTHDFFSTKGNGYQKGVYEELEDEDFII"
     gene            44365..44991
                     /locus_tag="ACOIAF_000996"
     CDS             44365..44991
                     /locus_tag="ACOIAF_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HGZ2668017.1"
                     /translation="MKYEWRKRDKELYLPKKNPVIVEIPEQKFIMLKGSGDPNSKEFT
                     EAIGVLYSLAYAIKTMPKKGIVPEGYFDYTVFPLEGIWDKGEKSKGLDVLIKEDLIYT
                     IMIRQPDFVTQDLLDKATDIVKKKKPHNLLEKVRFGSINEGLCVQMLHIGSYDNEPES
                     FDIMNEFCKKNKLIRKSYTHREIYISDARKVSPESLKTVLRFEVEREV"
BASE COUNT        17605 a         4800 c         7950 g        14786 t
ORIGIN      
        1 gaaagtaaaa ttttaaatat tgacattaaa ttaaatatac tatactatgt aagtgtgatg
       61 agagaatttt tttagagggg atttatagtg aatataaaaa agatatttaa taacaatgta
      121 gtagtaagct ccctagaaga tggcacagaa ataattgtca ctggtgctgg agtaggattt
      181 aagaaaaaag taggcgattt gatagatgaa aatttgattt ctaagaaata ttttgttcaa
      241 gacgatcaaa gggacaaata caatcaaata cttaataaga cctctattga atactttaaa
      301 atttcagaag aaattataga aaaggctaat gaagttttaa atactcaagt gaatgattca
      361 ataatattag ccttaactag tcacatagaa tttgctgtcc aaagagaaaa acaaggtata
      421 aaattaccaa acttaatatt gaatgagaca aaacaacttt atagggaaga atttgaattt
      481 ggattatggg caattgatga aattgaaaaa aggataggaa taaaattgcc tgaagatgaa
      541 gcaggatata ttgctattca cataatcaat ggtttggaaa attctcaaaa agatgaaggt
      601 attaatatac tagagttttc aaaacaagtt attcagataa ttgaagaagt acatggattt
      661 aaattggatg taaattcatt aaattatact agacttataa ctcacttaaa gttttttgtt
      721 caaaggattc tccgaaaaga aacttacaat gatagtgata ttgaagatat gtataagtta
      781 gtgaataaaa attacaataa acctaaagtg tgtactgaag aaattgcagc attggtatta
      841 gacagatttg aatataagat tagtaaagaa gaagaattat atttaatgat tcacataaat
      901 aaaataacaa acagtggttg ttaggattgt aactgataaa gcaggcaaaa cctaagtcat
      961 tgagcttaac tattagatta agttttttga tttaggtttt tttattagat acaatcaaaa
     1021 tacagacagt gacttttagg attgtaactg gttaagcagg caaaacctaa atcattgaat
     1081 ttaactatta ggttaagttt tttgatttag gtttttttat tagatacaat caaatacaga
     1141 cagtgacttt taggattgta actggttgag caggcaaaac ctaaatcatt aaacttaatt
     1201 attaaattaa gttttttgat ttaggttttt ctataaattt gattatgttt ttagataaaa
     1261 ataggagggg aaaattatga aaaaaaataa gttaggttta tggaacttct tccaaatgtt
     1321 aggaaaaacg tttatgttcc caatagcact acttagtgtt tctgggatga tgctaggact
     1381 tggggcaggt tttacagacc ctaagatgat tgaaatggtt ccatttttag ggaatgaata
     1441 tgttaactta gttttaaact tcatgttaac aataggatta tttgcattta acaacttggg
     1501 agcattattt gcaatggcaa ttccactagg attattaaaa aaagaaaaag agtttggtgc
     1561 tttttcagga ttagtaggat ttatagcaat gcacataggt acaaattttt atttaacaag
     1621 agctgatttg ttagtaccag cagaacagat gtcaactaat ggtcaagcta tgattatggg
     1681 tatacaaaca tataatacaa gtgttttagg tggtattgta gcaggattaa ttgtttatgc
     1741 aatatatgat aaagttagta atttaaagat accagaatca ttaggattct acagtggacc
     1801 aagattagtt ccaattgtta gtttaatagt tatgagtata gttggattat taatacctat
     1861 tgtttggcca ccatttttca atgcattcca aagtttagga agatggattt cttcagcagg
     1921 accattagga tatttttcat atgcagtggc agaaagagtt acaattccat ttggactaaa
     1981 tcatcttgta acatctgtat tccgttttac accaataggt ggaactgctg ttattgatgg
     2041 agaaacgtac tttggtacat tgaacatgtt tatggcatat gtagaaaata atcagattat
     2101 acctcttgat ttggctggta aaatggaaca aggtaaattg atgattcaat atggattagc
     2161 tggtgctgca ttggctatct accgtacagc aaaaccagaa aatagaaagg cacttaaagg
     2221 acttttaata tcaggagtgt tgacagttat tataggtggt ataagtgaac caatagagtt
     2281 tctattctta ttcatatcac cagcattatt tgctttccat acatttatga atggtctagc
     2341 aaacatggta cttccatact taggcgtgtt gatgggattc acaggagatt taattgcatt
     2401 tataagtttt ggtgtgttaa gaggaacagc tacaggatgg ccaatagcag tattagtagc
     2461 agttttatat tttgcaatat actattttgt atttaagtgg gcaattatta agtttaatat
     2521 aaagacccca ggacgtgagg ataaagaaat aaaagtatct gaaggaaatg gttcagattt
     2581 caaaaatttg tctacatata aaggacaaca aatgatatct gctttaggtg gtcaatctaa
     2641 tatattatca ctagataatt gtgtaactag attgcgttta aaactacaag atgtatcatt
     2701 aataaatgag gatgcaatta aagaggctgg tggtatagca gttgttaaat tagatgagca
     2761 tacaatacaa gtaattatag gaactcaagt gtattcttta agaaagcaga tggataaagc
     2821 tatggaaggt catttggagg catgtaatga ttaattttga taaaaaagta gaccgattgg
     2881 gtacatattg tacccagtgg gactatgtag aagatagatt tggtaagaag gatttgcttc
     2941 cttttacaat ttcagatatg gatttagagt ttccatttga aataacagag gtgttgcagg
     3001 aaaggttaaa tcatagagtt ttgggataca gtagatggaa acatgatgat tttagaaatt
     3061 cagttacgaa ttggtatatt aaaagattta attgtagcgt tgatagtaac tggatatatt
     3121 atagtccaag tgtaatgtat tcaattagca aacttattga gatgttaagt aatgaaggag
     3181 atggaatcct tataaatact cctgcataca atgcttttta tgatgtgata ataaataaca
     3241 atcgtaatat tataaagtca catcttgtaa ataggaatgg attctattca attgatttta
     3301 atgattttga gaaaaagtgt aaaaaatcta agatatttat tttatgtaat cctcacaatc
     3361 caactggtag agtatggagt gagttagaac ttgttaagat gataggaatt tgtaagaata
     3421 atgatgtcaa gataatatca gatgatatac acatggatat tgtatatgac aatagaatga
     3481 cacctatctt aaatgttgca aaggattatt tagattctgt attcatttgt acatcagcgt
     3541 ctaaatcttt caatatacca gcattgactg ggtcatatgt gattatacca aatgaaggta
     3601 ttagaaataa atttgaaaat atcactagat accgtgattt tgtaaattcg ccagcaattc
     3661 tagcaatatt agctacaatg gtctgttatg atgactgcga atattggtta gatgaactat
     3721 taaaatacct taaagagaat cttaaatata cgattgatta tataaaagat aatttagaac
     3781 cattgaaatt ggtcatgcca gaaggctgtt actttgcttg gatagatttt tccgaactta
     3841 aaataagtgg tgagaaattc caaaaattat tgatggatat tggagaagtt gctattatgt
     3901 cggggtctgt atatggtgaa gaaggtgagt tttatttaag gttaaatgtg gcatgttcaa
     3961 gagaaaaact tgaagatggt ttaaatagga ttaaaaaaac tatggattat attgcaaaaa
     4021 gaattttata gtaggaaggg agatagttat gggatattta aaaacaatgg ttgatgttat
     4081 gactactgaa atgaatgcca taagatgttt aatagatgaa gcaggaatag aatatgaaag
     4141 tatagttagt gaaattgcaa attgtgaagg caaagtaatt tttatgggcg taggaaaatc
     4201 tgcacatata ggtaagaaat tggcagctac atttgctagt actggtacac ctagcttttt
     4261 tgtacatgca acagaggcag tgcatggaga tttaggaatg attgaaagta aggatattac
     4321 aattttaata tcaaatagtg gaaattctat ggaagtggta aactgtataa aatatattaa
     4381 agcaataggt tctaagacaa ttgcatttac ttcaaacaga aattcagtac tggcaaaaga
     4441 gtgtgattat gctttaatat atcctgcaaa ggatgaagca gaccatttaa atctagcacc
     4501 aacaacatca tctacaatta ctttggtttt aggagattct attgcatgtg cattatctaa
     4561 gagtagtaat tttggttctt cagattttta taagtaccat ccaggaggaa gtttaggtga
     4621 gaagttaaaa acagctaaca atggataaaa gcaaaaagga gagtagtttt tatgttcaaa
     4681 atgtttaaaa aaaaatcaat aaaatcaccc atatcaggag aagtaataga gttatctaaa
     4741 atacaagatg gtgtattttc tcaaaaaatt atgggagagg gtgtagctat agattctaca
     4801 ggagacattg tatatgctcc aatcaatgga aagatagtag tagtagcaga gactaaacat
     4861 gcatttgtaa tagaattaga aaatgggatg gaacttctta ttcatgttgg tttagatact
     4921 gtaaatttaa aaggcgaagg ttttgaggta ttggtgagta tgggagaaca agtgaaggaa
     4981 ggaacaccaa tgttgaaaat taaccgtcct ttgattgaat caaagggtat atcacttatt
     5041 actccagtta caataactaa tcattcagag tacaatatga atacatgtaa tgtaggcaat
     5101 agtgttgaag gtgggaaaga tactgtaatt gagtttaaat aaagaactaa aatacaaggt
     5161 taatatttat aaatacctat aaacttatgt tttataagat tgattgtata gatttgttgt
     5221 ataaaaaaga actctttaat tataaattaa ggagttcttt tttattttgt aaatattgtt
     5281 tagatttata tagaaaaaca cttagtaata aatagaataa ataagtattt atatagctaa
     5341 aaatctaaat agttttgttt aaatatattt ttatattttg taatttcaaa ttaataaata
     5401 attagatttg ataaacaaaa aataaaaatg ttgattatat taataaactt taatataata
     5461 tatttatgaa aaatgtaaat tgtgaaatgg aggtagtaac atgaaaattg aaaatctttc
     5521 tattgatgat attaaacgag gctatatatt taatgatgat ttaaattgtt atagttgcat
     5581 taaatgtaca aaaacatatg aggtaggaga aatatataaa ataggtaata gattctttga
     5641 agcttcaaaa gctattgaac ttcatatgga attagaacat agtgattatt tagagcaatt
     5701 gcttaatagt gattcaaagt acaacacatt gactgaaaat caaaaacaat tatttcaatt
     5761 atttattagt gatttaccag ataaggagat agcaaaacag gttggagtat ccacatctac
     5821 tatacgccat caaaaattta ttttcagaga aaaagcaaaa caggctaagc tctatttagc
     5881 actatatgaa tctgtatttg aagataaatc taataaaagt agtactataa taccaatcca
     5941 tgaacatgct cgaatggtag atgaaagata tgtagtaact gaagaagaga gaaaacacat
     6001 tttggataca tcatttgaaa gtatgaatcc tttaaaatta aaagtatttt cttcaaaaga
     6061 aaagaaaaag gtcgtaattt tatctaagat tttggaacaa tttgaacgtg ggaagaaata
     6121 ttcagaaaag gaagttaatc agattttaga atctatatat gatgattttg caacaattag
     6181 aagatatcta attgagtatg gttttatgag tagaaataaa gaatgtacaa agtattggtt
     6241 aacataatta aaataataaa ttttactaat gtgtaatgtt atttttatct agggggagga
     6301 gtcaaatatg aatgatgaat taaaatctat tttatacaat aaaggtgtgg acattgttcg
     6361 ctttgttgat atatctgaat ttccaataaa tcaaacgcaa ggtttttcta aagcaatttt
     6421 attttgtata gggttatcaa aaaaatttat tacagatata tacaataact tacctacaga
     6481 tagtgatgag tttttggaaa aagaggagaa agtagaagaa ttagctgact ggatttcaaa
     6541 gtatattcaa aataaaggat atagagcgta ttctcaatcg gagaaaaaca atctagaaca
     6601 tggatatttt gaaaaagcct atattaatcc agaaatgcaa tcagggatta gtcctttacc
     6661 acataagaca atagcaaata ttagtggcat tggatttatg ggaaaaaata atttgtttgt
     6721 aacagaggaa tatggatgtg cttttagcat gtgtacagta cttacagatg ctccaatttc
     6781 tgttgaaaga tatcctttga ttgattcaaa atgtatggat tgtaatgttt gtgtagaaaa
     6841 ctgccctgca aaagctatac atggaaatga gtggactttg ccaggaaaaa gagagtctat
     6901 tattgatgtt tcaaagtgtt tttgtgtatt gaagtgtatg atgagttgcc catggtcatt
     6961 aagatatgca aatcaaaaat aactttacta gagaaggtat taaattgaat attataaaat
     7021 ttttaaaatg taagattata taagatttct caaaataaaa atttgtatag aacactcaga
     7081 aatgagtgtt tttctattgg gattaaatta tattgataca aagcttaact attttaatca
     7141 ttatataaaa tttgtattaa aaaattaaga ataacttggt aatagtgaaa atataatgag
     7201 attataattg atttataata taactataat gatattgtaa aaatgaaaat tgtcctctca
     7261 aaattcccaa agagagtaaa agtcacttca gttatagtca aaaaaatttt tctgtgaaaa
     7321 gaatttagat ttgaaaagga ggaattaatc atggaaattg aaactaaaac tccagattgg
     7381 attaatgtac tagaattaaa cagtaaaata gatattactg ggaaatatga tgtaagtaat
     7441 atgatacaaa atattgtctc tgataagtca ctagaaggtt ccattatata ttttccaaaa
     7501 ggtaattact tatttgagaa aggtattaaa attagtcaac aaataacact tcaaggagat
     7561 tcttattatg gaggaggtaa tcaagtatca aatttagata aaaaacaacc aatcatagga
     7621 acaaccaatt ttataacccg aggagtttct aatatgagta ttattacatt gtcaggaaca
     7681 agtcaatgta tcaaaaatat taatttttat tatgatagtc atgatataga gaaaataccg
     7741 cctaaaaatg tatctgctat tacagaatat ggagaaacac aaggattatc tcattttgaa
     7801 catctgttta tttctggatt ttcagggata ggaattgaaa ttccatacta ttcaactggt
     7861 aatgatatta ctgttagttc atgtggtctt ggtatgaggc ttggagagaa gtcaatgctt
     7921 tctagtagca agatttacga atgtaaaaat ggaatggaga ttactactgg tgttagctta
     7981 aataatgtga gaatagaaga aataaaagaa ataggtatta ataataaagg atttggtttt
     8041 aatctgatta tgaatcttat ggtcgaccaa tgtggatatt gtggatttat gtttgaaaaa
     8101 atgtactata gtcagataac tgccagaatt actagatgcg gtcagtatta taaaagtgtt
     8161 gattataatg cttatgagaa tatggaagat agaaaagaag aagcttattc tattttttat
     8221 ggagatattt tagaaaattg caatattgta ttgatgaata gcaatattga taccttagaa
     8281 agtgattcac ttaatgaaca taagatatat gtaatcaagg caaatgaaac aaacaatgta
     8341 atgctaacat gttatgctga agcagataac tttatacaat gtgcaaaggg caacttgctg
     8401 ttagaaaatg gtgaaaatac ttataaattt tataatggtg agatttgttc tgtaggaggt
     8461 gttcaaattt ctgatataga tgaaaaagat ttaatcaaaa taatagagaa cactttgtat
     8521 atagatgata aaggtaagta tttaatggtt cctagattaa aaaagaatac tgtaatatat
     8581 tcagtactag attctacaga agaaataaat aaattacatg gtggaatatg ggaacagatt
     8641 gatgtaaaag tattagctgg ggaatccatg tactattata aaaagataaa agattaaatt
     8701 atcaaactag gaggtagtga ttaatggaag agtctataat aattaaagat ataagtggct
     8761 ggattaatgt acttgattat ggaatttgta atgatgaaac aaaagatgta agttttaagt
     8821 tgcaagagat tatggatgct gtaccagaag attctattat atattttcca aaaggaaaat
     8881 acttatttga aagtggtgtt aagattaaga aacgattaac cttatgtgga gattcttata
     8941 ttttagcgaa tgttcctaaa aatactgctg gagtaacaca gtttactttt aaaggaaaga
     9001 aaaatcctaa aagtgaaaat atagcaatca taacagcaaa aagtgtaaga cactgtatta
     9061 agaacatttg cttttattca gatagttgtg atagagatat gacatctaat catgaacctc
     9121 caacagatgg aaaacctaga tttcatcata atgtaattat aaattataga aatatatctg
     9181 ctgttatatg tgaagataaa cagggagaac caggtcatta tgaaaatcta tcttttagtg
     9241 gtttttctgg aattgcactt gatatgccta ataattctac agcaaatgat attacaattt
     9301 ttacatccgg acttggaatt agtacagggg aaaattcaac aattgctaac agtaaggttt
     9361 ggggctgtgt tgatggaatg ataatttcta ctggaacttt tataaatggt atgagggtag
     9421 aagaaataaa aaaagttgga attaaaaata ttggtagagg tttaaatttt attacaaata
     9481 tcacaattga ccaatgtgga tattgtggag tttggtttga ttctatatcc aatgttcaga
     9541 tatcaggaaa tatcacaagg tgtgggcagt actattatga tgtaagctat gataattatt
     9601 taatgttgga taatagagtt aaagaagcat attctttatt ctatggtaat ttattggagt
     9661 caagtaatat agttttatca aataccaatg aagataattg ggaagatttc aaagaagata
     9721 agcataaggt gtatcttata gaagcactta aaaccaagaa tgtactatta acatgcaata
     9781 tagatgctac tgactggatt gttaagtcag aaaaaggaaa tttaacgtac aataatatgc
     9841 gacaaactgt tatattttac gatggaaaac taagtagtat caatggaatt ggtatatctg
     9901 atgaagatta tggggataaa gtcaagataa aaaaaggaaa tatatatata aatagtaatg
     9961 ataaggatat taagatgcat aaaccagagg taggtgacat tatgtcaaca attattaaag
    10021 ataaaagtat tctttctgct cattatgggg gaacatggga gaaaattggt gaaaaaatag
    10081 agtttggaat aactgtacac tattataaat tgatagagaa ataagtaaat acgtagtatt
    10141 tgaatagatg tattttattg gagagctagg actatagttt taatataaat ggtttggtaa
    10201 tagcatggat tgaataatat aaaaataata taaaaaatta atttgattat gtaaatttct
    10261 atgaagtagt aaaactgtga ctttagtcat aggaggttca aactaagtaa aagggaaagg
    10321 aatatataat attatggaag gaagaactgg gattaaagat ggaagatgga ttaatgtagt
    10381 agaaagaggt attaaaaatg atggttcgta caacataggt cctgctttgc aggatattat
    10441 tgatgaaata gaaacagatt tttgtgtctt atattttcca aaaggtaaat acctatttac
    10501 aagtggtgtt aaaattaata aagaaattgt attgcaaggt gattctaatt catctacagg
    10561 tactacacaa tttataactc gtggagtaga aaatatgagt attattacat tgacagggaa
    10621 gaaacaatgt attaaaaata taaatttcta ttctgatagt tgtgagatac aggtaaataa
    10681 agaaccaccg acaaaaggta atcctaaata tcattatgag atgattatta ataagaatga
    10741 aaagaatgtt gagatgaata atgtatcagc aatcctgtat gataacagta agaatatgat
    10801 taaaggtcta ggtcattatg aaaatattta tataagtggt ttttcgggta caggaattag
    10861 gataccatat tactcaattg taaatgatat tacagtttca tcttgtgggc tgggtattga
    10921 tacaggaata gacacaatta tttctaatag tagaatatct aagtgtcaaa atggaatgag
    10981 gattagaact ggaactagta taaataatgt aaggattgaa aaaatacaga aagttggtct
    11041 tataagtagt caagttaagg ggtatgaggg gtatggaagt tataaaatta ataatattac
    11101 gattgaccaa tgtgggtatt gtggcttttc atttgattca atgaaagata gttatttttc
    11161 tggtattata agaaggtgtg gtcagtatta ttataataca gattatgaca catatttaag
    11221 tattaaagat agagtagaag aagcatattc tttattttat ggaaattatt ttacagaatg
    11281 taatattgat ttagttagtg ataataagga taactgggat gatggcttag aatataaaca
    11341 taaagtttat gtatttaaaa cccttcaaat accaaaatta acattgagat gcaatataga
    11401 tgcctatgat tatatcgtta aatcggtaca tggaggaggt aatttaatgt taaaaaataa
    11461 ctttaacaat tatatattta ttgattcatc taatccagaa gttattaatg gaattaattt
    11521 taattatcaa aagaatggaa acttgttaag gttaaataat ggtaaattaa gttataaaaa
    11581 tggaaaggaa gaaacagcta tattcagacc taattatggt cttattgtag catcaacaga
    11641 ttcagaatgt gaacaaatag agactagcta tggaggaaaa tgggagaagc ttggttctga
    11701 agtaattgga gaagaaacgg tatattatta taaacatcat gaaccagata aaacaaacca
    11761 aatacaataa aatttgtatt atataatgat ataaagtttt aatatatagt gtttaccttt
    11821 aatgtaatat tggtatattt gtatagtatt aaatttgtta aaaagtaaaa ttgtagggtg
    11881 cttgaaaata gcaccttttt tagtttcatt ataaaattag ttttgaaagt ttaggaataa
    11941 tgtatattta tttttaaaga attataacct tattaaggat attttaaatg ctagaaatga
    12001 atcagtaaat catactggaa ttatcttaaa tgttttgatt aaattaagta tttagagcta
    12061 taattatatt aaaaatatat tagaacataa ttatattact atggaggtta aatatgatag
    12121 taagtgtgag tagaagaact gatatacctg ctttctatag taagtggttt tttaacagac
    12181 ttgaagaagg ttttgtttat gtaattaatc caatgaatcc aaaacaggtt agtaagatag
    12241 agttaaatcc acatactgtt gattgttttg tattttggac aaaggatgta actccaatgt
    12301 tatatgattt aaataaatta aaagactata aatattattt tcactatact ataacatcat
    12361 atggaaaaga agtagagaca tgtattttag ataaaagaaa ggtaatagat tcatttaagg
    12421 aattgtctag aaaaataggc aaagaaagag taattttaag atacgacccg attttcttaa
    12481 gtgaaaagta cacagtagat tatcatatca aagcatttgc gagtttatgt aatcagcttg
    12541 atggatttac agaaaaatgt ataataagtt ttatcgattt atacaagaaa acaaaattta
    12601 atactaaagc attacatata aagccaatag aaattacaga aatagaaaca ttatcaaaag
    12661 agatttctaa gattgctcat aagcatggga taacacttga aatgtgttct gaagaatata
    12721 atttaagtaa atttgggatt ggaaaaagta agtgtattga tgatagatta atatcaaaaa
    12781 ttataggatg tgaagtagag gtaaaaaaag atagtacaca aagagatatt tgtggatgtg
    12841 ttaaaagtgt cgatatagga caatacaata cttgcagaca ttattgtctg tattgttatg
    12901 ctaactttaa ttatgcaaaa gtagaagaaa actgtaggct ttataaggaa gataataaat
    12961 tactcataga tactttaaga gatgatgcaa aaataagcat tagagatatg aagactatta
    13021 aagtagataa taataaacaa attagtatat ttgattaaaa taaatatact aattacctta
    13081 atatattaag aatatagata cttttgataa taaaattaaa cttaatagat attttctata
    13141 taagaaagat ggtcataata tgtcaatttg accatctttt tgatataatt aaaacacaaa
    13201 taatttagta acaaaggagg cagaacttga atttattaaa aaaatatcct atacctattg
    13261 caagtttgat tttatcaatt tttactttag gtaacttatt acaatcttat agtgaatcat
    13321 tacgtaatgt aattggattt ataggattta ttttgtatgc aatttatata gtcaaaatag
    13381 tattacttag aaagggtgtt aaagagcaat tagagaaccc tttaatatca agtacatttc
    13441 caactataac aatggcaaca atgatatttg ctacatatat aaaaccatta tctttagaat
    13501 taagtattat gatttggagc ataggaattg tgggtcatct tgtgttaata gtcttattta
    13561 gtaagaagtt cttagttaaa ttctcaataa aaacggtatt tccatcttgg tatatagtat
    13621 atgttgggat agttgcagct agtgttacgg caccagcatt taatcaactt ttgattggca
    13681 aaattgcttt ttggatagga tttgtatctt atatagttct tataccagta attttaaaga
    13741 gagtatggat aatcaaggaa atgcctgaac catctttacc aagtatagct atattagctg
    13801 caccagggtc actattattg tctggatata ttaattcttt ttcagataaa aataatgtag
    13861 tattatactt gttattcata ttatcaattg tattttatat tatagttttg ggatatttac
    13921 cgaaattatt aaggttgcct ttttatccga gttttgctgc atttacattc cctatggcaa
    13981 taagtgcaat gggtatgaaa ctaatgaatg gttatttaac taaatcaggt cattttataa
    14041 gttggatttc atatgcttct aagttagaag agataattgc tacagtgatt attttatatg
    14101 ttttagtttt gtttattata taccttagta aatctgaaac tacatctaaa gctaaataag
    14161 aattattaaa tatgtgtatt taataggaat attgatataa atatatgaag ataagataaa
    14221 ccatagaaac ttaatttagc ttaattttat tggaggaaaa caaatgaaaa caaaagaaga
    14281 atatttaaaa gaagttagaa acatatcaga agaatacttt agaaaaggag agtttttttg
    14341 ctctgaagca gttctccaga caataaatga tgctttaggc caacctttaa gtccagagat
    14401 aactaagtta gcatcaggat ttccaatagg acttggaaag gcacaatgtc tttgtggagc
    14461 aatatctggg ggagagatgg ctttaggtat agtatatgga agagttcatg gtgaagctat
    14521 gaatccaaag atgtttgagc atgctaaagg tttgcatgat tatattaaaa aagagtatgg
    14581 tgcaacatgt tgtagagcta ttacaaagaa atgggatggt gataacttta tgtctcccga
    14641 aagaaaagag cactgtatta aaataacagg tcaagttaca gagtgggttg cagataagct
    14701 tatagaagat gaaaaactta atatcaaata gtgtaatatt agttggtgaa tttataaagt
    14761 taaatattac ttatagtata agatacttga tattctcgta ttgatagaaa aatttatagc
    14821 atatgtattt aatgaaatat acttatattt agagtgtggt ttaatttcat aaatacatat
    14881 gctattattt taagtatata agcaaattta tttaaataaa tagaaacagt taatttatat
    14941 gtacaaaact atatatgatt agtgtaaata aatacctatt ttataatact caacataaag
    15001 acaccagaca tgattagtat tataccaata aaaatagcaa gccattcttt tttgttcttc
    15061 ttttctttaa aaaatataat ccctcctaaa gtggctacta tcacacaact ctgagagata
    15121 gaaaaactta ttgtaactcc caatgtatta gtcgttatga acatacctaa atttgctatt
    15181 gaccacaata taccagtaat taaattgaat aaaacatttt ccctatatac aatctcttta
    15241 gaaaaatact tgacaataat agatgaagtt atcaacatac caacagattg aggtaatata
    15301 attctatatc cttcaattaa aaatatttga ttagttacaa catataatga taaaaataga
    15361 gatgataata atacaactcc aacacatttt aacatatttt tatctgtaga ttttttagtt
    15421 tgataatttg tgagaagtat accaagtatt atacataata ttgataaaga accaatcagt
    15481 aacattttag aactggtcca ttcattaaac aagattacag ctaataatgt cgtaaaagtt
    15541 aattgtgaac catttgaaat tggcattact ttagatacac tagcaatttg aattgcttta
    15601 aactgcatta attgtcccaa actccagaat atgccagaaa tcatacttat acaaaatgaa
    15661 aaaaatgtaa tttcaggatg tataaccaga gttattatag tggaaaatat aaacgcactt
    15721 atggatgtac ctaaaagctg ttcatgagga gaacttttct ttagatttgc aactatgggc
    15781 atcaatcccc atcccattac aggaagtaaa gcaataatta ttgtcagcat taaactattt
    15841 cctttcatat taaatttaga cagcttttaa tttatcatct aaaattaaag tgttcaatac
    15901 ataaaaatta ccgtatttaa tcaggaaata ttagtagttt tttagaaaaa cattttccat
    15961 taaaataaag acaaagggaa ggagtaaaat tgaaaatgtt aactgatatg attcaaaatc
    16021 gtgaaattaa aataataaag tttttattaa aagaaggtca tacaactgta aaagcaatag
    16081 cagatgattt agatgtttca gaaaaaacag tttcaaaatc tcttaaagaa gtagaagcag
    16141 tgcttaaaaa gataggtctt tctttaataa gaaaacctaa gataggtgtt tatattgatg
    16201 gagatttaaa aaaaatactt ccttacctag ataataaagg aagtcaaatt cctacaacca
    16261 gagaggagag agttatctat atatattcag tacttttaaa gagtaatgat tatattacta
    16321 tacaacaact ttctgatgaa ctatatataa gtcgtggaac tattgaaagg gatttagcag
    16381 aagttgaaaa actgctaaaa aacgaaggga tttccatata taagaaacct agtaagggaa
    16441 taaagttaaa tataagtgaa agagataaaa gacaacttac tgctaagttt atacataaat
    16501 tttggagtag aaactggtat attaagcaag agcaagaaag cttttatcaa gcatttgaaa
    16561 agatacaggc agatgtaaat ggtatttttt cggaagatga tttatcaata attattgata
    16621 ttcttaggaa tttcagcaga gaaagaaaat ttgagttctc tgattatgaa tttcagtcat
    16681 tagttattca tttggcaata gctgtagaga gaattagaaa aggcgaatat atagaagaaa
    16741 gtttgtatat tggtaatata gaaaataatc aaatgtcaaa taccaattat ttagcaaaac
    16801 aattagaaga aaaattttca atattattac caaaatctga aattggatat ataaatttac
    16861 atttagtagc gtctgaccaa catagagata ataatgaact aaaaaatact tcaactaatc
    16921 cttgtgagac aagtacaaat aatatatcaa atcttcggga aattataaaa aatacattgt
    16981 atgagacagc ttatgacaag gatttaattg atggattgac ttcacatgta cattcatcaa
    17041 taaatagact gaaatatgga cttagtatta aaaatccata tgtaaataca atcaaacaaa
    17101 acttttcttt atcatttgag caagctttag atttaaagaa agtaattgaa tttatatatg
    17161 atattaggat aaatgatgat gaatgtgctt atatagcatt acattttgaa gcttttagag
    17221 aaagaatgaa agaacttcca caaaatatta gagtaattct agtatgcagt acaggattag
    17281 gttcatctag gttattgtct gcaagaataa aaaaatactt tccaatgata aagattgaaa
    17341 aagtactttc cgtacaagaa ttagtatcca gtaaaattga tgcagattta gtaataagta
    17401 ctatttattt agagttagaa acaattccaa ccatagtagt tagtcctatt ttaaatgaaa
    17461 gtgatataag aaccttggaa agaaatatac aaaatgtttc aagttgtact tctaagaagt
    17521 ataaatcttt cagaaattta atctttgaag aaaatatatt gttgaatatt gaagcttcaa
    17581 attatgaaga tgcaattaat catataacag ataacctaat aaaaaaaggt tttgcaaaag
    17641 aaggtatagc taaaagtgca attgagagag aaaatttatc ttacacgtct ttccagtcaa
    17701 tagctacacc tcatgctaat cctagttata ttaaaaaatc taatatatct gttgcaacgt
    17761 taaaaaatcc tattacatgg ggaaatgaaa aggtggaagt tattttcttt atttcattag
    17821 aaaatgacaa aaacctagag cttgatgaaa tatatgaagt tttttatgac tttattgaca
    17881 ataaaaaaaa tataaatgac atattaagtg caacttccac aaaggaagtg tacgatttgc
    17941 tagtggagga gagtatatag tgaaaatatc agatgtattg aaaaaagagc aaattgtttt
    18001 taatcttgat acaaatacaa aggaagaaac aataaaaaaa ttaagtagta tatttgttga
    18061 aaatgggatt gtggacaatg aagaagaata tgttaagtct gttttggaaa gagaagaaca
    18121 ttcaactaca ggtattggta atggtattgc tattcctcat gggaaaagta atgctgtcaa
    18181 aaaatcttct ataatatttg caaagactaa gcataagatt gaatgggatg ctttagatga
    18241 aaaaccagta gatagtattt tcttattggc tataagcaat attgataagg atagcaatca
    18301 tcttgtacta ttatctaaat tagcaactaa acttatggat gatgacaatg tagatgcact
    18361 aaaattagcc aattcagaac aagaaataat caatatattt agtgaagaag gagagatgta
    18421 gaaatgaaaa gaaaaattat agcagttaca gcatgtgcaa caggagttgc acatacatat
    18481 atggcagcac aagcattaaa gaaagcttca aaaaagatgg gtcaccttat aaaagttgaa
    18541 acacaaggag ctacaggaat cgataatgag ttaactacaa aggatgcaca aattgcagaa
    18601 gtagtaatat ttgcagtaga tactaaagtc agaaatgctg aacgttttga gggaaaagaa
    18661 atactagaag taccagttaa tgcacctata aaggatgcag agaaagtaat aaatgatgca
    18721 ttaaagttaa ttgacactaa ataaatggag ggaaatgtaa tgttgaaaaa gatagtctct
    18781 gagttaaaaa agcatgtctt aactggaatc tcatatatga ttccattagt aattgcaggt
    18841 gctatgataa tggcgatatc tcgtgtaggt ggttctttct ataatatacc agatatatgg
    18901 gatgctaaat atgcagaaag tgctagtagt attgttagat tattacatga tttagatggg
    18961 tttggaggta ctgcattagg attaatgttt cctgtaattg ctgcatttat aggattttct
    19021 atagctgata aattagctat tgtaccaggt cttgttggag gtatgattgc taaagatata
    19081 ggtgcaggat ttttaggagc tttggcagtt ggtcttatag caggatatac atgtttgttt
    19141 attaaaaatc atgttaaatt accaaaatca gcagcttcaa tagtaccaat atttatagta
    19201 ccagtatttg ggacattaat cactgttgta ttaatcaatt atgtgatagg tatacctttt
    19261 gccacattaa atactggatt agaaaattgg ttaaatggat tatctggtac aaatcagata
    19321 ctaatggcag ctattatagg ggctatgatg gcagttgact taggtggacc agttaataaa
    19381 gcagctttaa caacatcact tgcattatta acaagtggaa tttatgcgcc taacactgct
    19441 gcaatggtag gaatagttat accaccacta ggattgggac ttgctactat actagcaaaa
    19501 caaaaatata ataaacagtt acgtgaagct gggaaatcat ctcttataat gggtttaatt
    19561 ggagtaagtg aaggagcaat tccttttgca gtagaatctc cattaaaagt aataccttca
    19621 tgtgtaattg gaacagcaat tgcttcagct atggcagttg gtttaggttc agtaaatgca
    19681 actccaatta gtggatttta tggatggttt acagtagaga attggccaat gtatgtgtta
    19741 tcaatagcag ttggaacagt aattgtagct ggattagtta tagtattgcg tggaaatcca
    19801 gtgattgagg atgatgaatt tgaagatgat gattttgatg aagcagagtg ggaatcttaa
    19861 aatttaataa ctatttaaga cgtgttataa gctatattca atatgaatct tatactaaat
    19921 aaagtgttaa atattcagtt tagacttaaa tataaaagat aaagaacaga ggagaataaa
    19981 tgataaaagc acatattgtt aatcatacac actgggatag agaatggtat tttacttctg
    20041 gagatgcatt agtacttagt gaacaactat ttacagatgt aattgatgaa ttagaacgta
    20101 atccagatgt aagttttgta cttgatggac aactttctat tttggatgat tatgtacaat
    20161 tacacaaaga aaaaatagaa atcattaaaa agttaataaa agaagataga ctacatattg
    20221 gaccatggtt tactcaaact gatgcattct ttgctagagg agagtctatt ttaagaaact
    20281 taatgattgg aatttttgaa agtaaaaagt atggtaagta tatgaaaatt gggtatttgc
    20341 cagatacatt tggatttaat gcacagatgc ctatactatt aaagcatgta ggtcttgata
    20401 atatattctt ttggagaggt atacatcttg gtaaacaggt tcaatcacca tattttaagt
    20461 ggaaaagctt aaatggagaa agttatatat atgcagtaaa tatgccacat ggatatggaa
    20521 ctggtatgct tttagagcct tcattaaagt atgttaatgg acgcttagac ccagctattg
    20581 attttattaa atcatatact gatgtagatg aggtgttaat accttctggg aatgaccagt
    20641 taaatataat aggtgatttc aaaaataaaa ttaaagcgat taatgatatc ggaaagtatt
    20701 catatataac gagttcatat caagagtttt taaaatatat aaagtccata gaatcactag
    20761 aaagctatag aggtgaattt agagaacctg tattagcaag aatacataaa tcaattggtt
    20821 ctagtagaat ggatataaaa ttagcatgtg ataaactaga aaacaagctg attaaaagaa
    20881 tagagccatt attagtaatt gctaaaaaga gtaaaattga aataagcaat cagttactta
    20941 taaatacatg gaaaaaactt ttggaaggac aagcacacga tagtttagct ggatgtgtaa
    21001 ctgatactgt aacagatgat attcttcacc gtataagaga agcaaatgaa atatgtgaca
    21061 gtattgaaaa tactattgta aaaaagattt cagaagggct taaattatct aaaaatgata
    21121 ttttagtatt taatacagaa gctaagcgtt ttaatggata taaagagata caagttgtaa
    21181 gtgatagtaa aaatatttat tttaaagata atttagatgc tactataatt gaagaaactt
    21241 atgtaaaacc gagagaaaat gtattagaag aaacacctgc tggtaatata tttattgaag
    21301 aaccaggata ttatatatta aaagtaagaa taaatataca gcttcctgca cttggttata
    21361 aagttgttag ttttgaatta agtgataaag aaatggtgtc acttgataaa tctaatgaca
    21421 cttttatatc taatgataat tataagataa tatatgaaga tggaagctta aatttacaac
    21481 ttaaagatgg aacttacata tcaaactttt taacattaaa agatagtggt aatgcaggtg
    21541 atacctatga cttttctcca ctaaaaaatg atgaggatat aaaactttcg tttgataaag
    21601 tgtatacaga aaaagcttta ggatatgaaa aaatggtggt taaaggtact actttactac
    21661 cacttacatt agaagataga aaaaacaaat atcttaatgg gaaattaaat gtaaaagtaa
    21721 gtattgtctt atccaaagaa aatccattga tggatgtaaa actttgtgtt gataacacga
    21781 tatataatca tagactacgt gttcatataa aaactgatat aaaagataat aaaaacatag
    21841 catcattgcc atttggatat ataacaagaa aaaatggagt tttagacaac tgggaagata
    21901 tttacagtga aatgcctata gatttagaac ctttggaaag taatattact ctaacaaacc
    21961 aaaatagaag ctgtactata tttactagag gaataaaaga gtatcaacat attgaagatg
    22021 aaattgcatt gaccttactt gctactacag atgaactagg aaagcctgat ttattatata
    22081 gaccaggacg tgcttctggt gatacaacta aaaaaggtca tataagaatt aaaacagata
    22141 aagctcaaat gcttaagaaa ttagaatttt catttgcaat atatatggat tctaaatcat
    22201 ttgatgaatt ggaaattgcg aatttaaccc acaattatct aagggaatgt gtaaattatc
    22261 aattacagga ttacaatttc tttttatatc gtatagataa taagattcaa aaatcaattg
    22321 tcaataaaac tgtaagtaga gaattagaaa taatatcttt accagaaaac tatcttatat
    22381 cagcatgtta tccttcttat tacagtaaag ataaatttat aataagagtg gaaaatccaa
    22441 gtagtaatag aatgatattg gatgagcaaa tatttaaaaa tagaaatggg aaaattgtaa
    22501 atgcaataga agatgtagaa gaggaacagg tatatgaaat agcacctttt gacgttattt
    22561 caatattatt ggatttataa gtgtaaaagt aagtttattg tttaaagtct gacagatatt
    22621 aatctgccag actttttaat ttgtataaat ttgggtaaat attaatgaat ggttttagta
    22681 tttaagtatg aatttagtat ttaatataga atttattgaa gtagaataat tagtttaaca
    22741 ggataagcag tttaaataaa gaattattat attagagtga ttttatatat aatttaaaat
    22801 aataaaattt taataaaatg agttaaagat tgtatattaa tagagttgat gtagttacaa
    22861 aaaagttacc aattgttatg tatttacaaa actgatatat taattacata aaatctaaaa
    22921 tagtttgtaa taggagtata tataataaaa attttaatta aataaagcta ggtatattta
    22981 taatattaat aaatatattg ctataaaagt ttgtttaata aattaaataa agaaaagagg
    23041 agaataaaat gtacaacata gatgatatga tatataattg tccagtagaa gctttatcaa
    23101 atattttagg taaaaaatgg gtggctataa ttatatggaa aatacaagaa gataaaaaac
    23161 gatttggaga gctacaaaga gctataccag actgtagtaa gaaaatgtta gttcaacaac
    23221 tagatttttt aattgaacag gggattatag tcaaccaaaa aaagttggtt aataatgttg
    23281 tagagtcaac atatttttta agtgaatcag gattacgatt gcttcctgtc atggaaaaaa
    23341 tgattatgtg gagcaataaa aatttaaatt gcgataaata ctaaaattta ctctgtaagt
    23401 aatgaaaatt taattaagct atgagtagaa aaatgtatta aaattacata attttttagt
    23461 tagattttaa tttagaaata ggattgtaat attggaaata aaataaggag gaattttaat
    23521 ttgcaaaatg taaatttatt tttagtattt atagaaggta tagtatcatt cttttcacca
    23581 tgtatactgc caattttgcc tatttatttg agtatattgt caaatagtag tgtagaaaat
    23641 ttaaaagaag gaaagacgag ttttatagga agttctttat ttaaaaatac tatatttttt
    23701 gctcttggaa tctcaacaac attctttata ttgggttcat cagtaaaagt attgagtatg
    23761 ttttttaatg aaaataaaga tttaataatg tttattggtg ggattataat tattatcatg
    23821 ggattatttt acatgggaat aattaaatct tcaatattga atagagaaaa aagatttaat
    23881 gttaagttta aagaaatgaa agcaataaca gcttttatat taggttttac gtttagtttt
    23941 ggatggacac cttgtattgg tccaatattg gcatctgtat tagtcatggt ttcaagttca
    24001 agtaatcatc ttagtgccaa tcttttaata gcagtatata ctattggttt tattttgcca
    24061 ttcataatta cagctatgtt ttacagtaaa ctatttaaaa ctatagataa gataaaatct
    24121 aacatggaaa ttattaaaaa aataggtggg attatactta tagtatcagg aatattaatg
    24181 atggtaaatg gatttggaag tataagcaaa cactttaata cgtcacaaaa tagtaaaatt
    24241 gaaagcaagc aagaagaaaa taagcgagaa aattctactg ataaagaaga aaattctgat
    24301 ggtaatgata gtcaaaaaga ctctaataat gacaataatg ataaagggtc taatgatgaa
    24361 gatagaataa agtctattga ttttacttta acagaccagt atggtaaaac acataaatta
    24421 agtgattatg aaggcaaagt tgtattctta aatttctggg ctacatggtg tcctccatgt
    24481 aaagaagaaa tgccttatat agaacaactt tataaagact ataataaaaa taatgatgat
    24541 gtagtaattt taggagtagc ttcacctaat ttaggtagag aaggttcaag ggaacatgta
    24601 gttaacttct taaaagacca aggttataca ttccctgttg tacttgatga agatggagca
    24661 ttagcatatc aatatggaat taatgctttt ccaactacgt ttataattga caaggaagga
    24721 tatgtaactc agtatattcc aggagctatg gataaggcaa ctatggcatc atttattgaa
    24781 aatcagagaa ataaataaaa gtatttaagt ataagtttat gaatcaattt aaggttacaa
    24841 aaaagtgcct aattgtacca cctaaataat cttgttatag taaaagtata aacaatacaa
    24901 gaaagggttg aattttatat gaaaaaaaca tttgatgcta taataatagg atttggaaaa
    24961 ggtggaaaaa cattagcagg ggatttggca aacaggggat taaaagtagc tttaatagaa
    25021 aagtcaaaca agatgtatgg tggaacatgt gtaaatgtgg catgtatacc aacaaaaagc
    25081 ttagaaaatt cagcaaacag cgtaaaaaca aagaatataa attcttggga tgaagtacaa
    25141 gctgaatatg agaaagctat agataaaaaa gaaactttaa ttacaaaact cagagaagct
    25201 aactataata aattaaattc aaatgagaat gtaactatat tcacaggaat gggaactttc
    25261 attgatgaaa aaacagttca agtcaaaact gaaaatgaaa tttatgagtt agtagcagat
    25321 aatatattta ttaatactgg ttcaagacca tttataccta atattaaggg aattgaaaat
    25381 aagaatatag tatatgatag tgagtctctt atgaatctaa gagttttacc taaaaaaatg
    25441 acaattatag gagctggatt tataggttta gaatttgctg gtatttatag ttcatttgga
    25501 gcagaagtta caatattaaa ctctaataat ggcattttac caaatgaaga tgttgaggat
    25561 tcagaagaaa taataaaatt acttgcaaag agaaatgtaa aaattgttaa taatgcaaat
    25621 ataaaagaga ttaaagaagt ttctgaatta gcaatagtag aatatgaagt agatggtaaa
    25681 tctaaagaat taacaagtaa tatgatactt gtagctacag gaagaaaagc aaacactgaa
    25741 gggctagggc tagaaaatgc aggtatagaa ttaaatgaaa gaggatttat taaagtctca
    25801 gaaactctta agactaataa agagcatata tgggctatag gagatataaa tggaggtcca
    25861 caatttactt atatttcatt ggatgattat cgcatagtta ttaatcagtt atttggagat
    25921 aagactagaa caactaatga tagaaagaat atcccaaatt ctatatttat aagtccagca
    25981 ttttcaagag ttggtcttaa tgttaagcaa gctaaagaaa agggatatga agtattagtg
    26041 gcaaagatgc cagtagaagc aattccaaga gctaaacaga ttggcaaggc agatgggttt
    26101 ataaaaatag tgatagataa aaaatccaat aagatacttg gagctagtat gatatgtgaa
    26161 aattcaagtg agattatcca tcttattcaa ttagctgtag atttagaagt agagtataca
    26221 tatttaagag accgtgttta tgcacatcca actatgacag aagctcttaa tgatattcta
    26281 tctcctaata tgataaaaga agtataacat aagtttaata tataaaccta aaattatcat
    26341 tataagtaga ttttagaaag aggctatctc aaaaaataga gataagtctc ttttttactg
    26401 tttaaatgat aaaaatatat ttgattttat aaaagaagat tatcttatga actaaaaagt
    26461 ttattttaaa acactaccta tttatgttat aaacttataa atataatata gaatctatta
    26521 ttctgtatta ttaagtgggg ttaagcgtta taattaatta taaatattga ataatctaaa
    26581 tggataagat aatggtatta aaaattatct taaaaggtga aataatctgt atttaatgta
    26641 gtacagttat atctttaaag caaggtggtg aaatgatata aataagatat ctaaaagagg
    26701 gtttgatgat agtgataaaa gatggttttc aagtaaagaa ttaacaagac ttataaaggc
    26761 taaagaagaa atagaatggt tattaaatag ggattacaaa atagacccag tcgttacttt
    26821 tgttggtgat agatatcagt tttctataag acaaagagat gcattaaaaa gggcagtctg
    26881 tactgatgaa aaaaatatta taagacaatc aaaaaggctt tctctagata aaatcaaaga
    26941 agataccgta aatatagatg gctttaattt gataatatcc atagaggtag cattatctgg
    27001 gggtactcta gtaataggaa gtgatggcaa tataagagat ttagcaggtc ttaggggaac
    27061 ctataagatt atagataaaa cagaagaagc tttgaatctg ataggtaaat tttttaataa
    27121 atacaatgca cagaatctta aattttattt agatgctcca gtttctaatt caggaaattt
    27181 aaagtatagg atattagagc atgccaaaac ttggggaatt gaaactgaag tagaattagt
    27241 aaaaaatgca gatgttgtat tggaaaaatt agatagagta gttagtagtg atgcagtcat
    27301 agttgacaag tgtataagtt attttaatgt ggctagaggt ataattgaag agtatattaa
    27361 agaatgtaat atcataaatt taaataaata gatagatata ggtgagaata tataagtata
    27421 tgtagcaata ttcttagtaa tacaagtaaa gtttgaaaat aggagaacga atacatatcg
    27481 aatttcataa aatgttgtaa aaagacatat gagaggatgg atatgtgtga aattaactaa
    27541 actaaatatt aaaaaatata gagcacctat atataaatat gctttagcaa atgtaaattt
    27601 gaggtctgct aaatctacta attcaagtat cataacagta atacctcagg gagccaaaat
    27661 ggaagtattg gatgaagaag atgactggat taaggtgatg tacaattctc aagaaggata
    27721 tgtgtataag gatttggtat cagtaagcga atatgcttgg agtaacttaa acttaagaga
    27781 agataaatct acaacttcca atataattac agtaattcct gaaaaatcta gggttgaagt
    27841 ccttcaagta gatggagatt ggagtaaggt tgtatatgat gataaaatag gatatgtttt
    27901 taattatttt ttatcaattg atgggaataa accaaatgaa ttagattata agtattttta
    27961 tactgacatg attaaatttg ttaacgaaaa taatattaaa agtactagcg attatctaat
    28021 tgtaacagac cttagaaata aatatactta tatttttaag aaagataatg gaggttgggg
    28081 acaactttac aagtggcaat gtaccatagg taagcctgag actccaacta taacaggtat
    28141 attttatata agtggaagaa aaccttcttt tggaacggat gagtattcag ttaagtatgc
    28201 tactagaatt aaaggtggat attactatca ttctgtacta tatgattcaa caggaagtta
    28261 tataatagat ggcagacttg gtgaagctct tagtcatgga tgtataagat taagtacgga
    28321 aaatgcaaag tggatatatg ataatatacc agatacaaca acagttataa ttcattaaga
    28381 aaaattttga gttttttatt gggataataa ttcttaaatt aataggatta tatattaact
    28441 tctcataaag agtactattt aaaaagtgta ataagagagt gctattaaca aaacaaagat
    28501 attttgaggc actctcttat aaatatacat aattaagata ttttcataaa caattctata
    28561 atcttatctt tatcaaagaa aacaggatta gcaccagcac aagcatcttt catagagttt
    28621 tctgcaagca attcaaaatt aggattatca acacctacat cttttaaaga ttttgggatt
    28681 ccaacctctt cagaaagagc ttttatctta tctattacaa agtcaacaca ctctttatct
    28741 gatttattat ttacatccat tccaataact tcagctattg gtcggaattt ggcaggggct
    28801 gatttagcat tttcctcctc aactattgga agtagcatgg cattacatac tccatgaggt
    28861 aaatcatata aacctcctaa ttgatgagcc atagcatgta catttccaag acctgcatta
    28921 ctaaaggcaa ttccatttaa aaaacaagca taacacattt gttctctagc ttctatgtca
    28981 ttaccatttt taacagctct tggaaggtat ttaaatatct gctttatagc atataaggca
    29041 gttgaatctg taacatcata agcaccatta gctactacag cttctatagc atgagttagt
    29101 gcatccatac cagttgctgc tgttaaatta gatggtttgc taatcattaa ttctgggtca
    29161 tttactgtca ttgtggctag tgaattggta tcaaccatta tcatctttac atgtctgtct
    29221 tcatcagtta taacatagtt tattgtaact tcagcagatg taccagcagt agtagttata
    29281 gctatgatag gaagacattt tttagaagtt ttgttgatac cttcatagtc tctaatatct
    29341 cctccattag tggctaatat agagatagct ttaccacagt cttgtggaga accacctcct
    29401 atagttataa cgaagtcgca attttctgat tttaatattt taagtccttt attcacatta
    29461 gttacagttg gattttgttt aacttcatca taaacaacaa agtctatatc tatctgagat
    29521 aaaatatctg ttactttttt tacagtacca tttgaaacta gaaatttatc actaacaata
    29581 aatgcttttt tagttcctaa tgatttaatt gaatctttag cttcattaag acatcctttc
    29641 ccaagtaaat ttattggtgg tacataataa atgctcataa ctaaaatctc cttttatttt
    29701 cattgatttt tatgtaaaaa tatatatcta acaattttca ctaatttact cataatatat
    29761 cgtatcttag attgagatag ggttgttggt tcttatataa gaacttgatt cgtaattacg
    29821 attatgatta gaatttaaca tagtatcttt atcatgtcaa taaaaatata aaaaatatta
    29881 taaaaaaaac aaagttactg tagttaaatc agcaactttg tttttatagt ataaatcaaa
    29941 taaatacatt agtttaaaac aaaattttta attattatgt tttgatatct tcaatataca
    30001 gaatattaat ctaatttatt acaatgtctt cattacgttc ttccatcatt gattgaatat
    30061 ttttctgagc atttaagagt aattttttta tcagttctat tttttcgtca ttagctctaa
    30121 ataaaggcag tgttacacta acagcagctc ttatacatcc attttttctt attggtacgg
    30181 caagacattt taggtgttcc attgattcct cacactctga agctatttgt gttctttgaa
    30241 cttcaagaag ctggtcgtat aaaacatcaa aatctgtaat tgatttttct gtaaaacctt
    30301 ttagaccatc tggatataag cttattagtt tttccttgtc aaaatcacat aaaagagctt
    30361 ttccaagacc tgtacaatta gcaggtattt tttttccaac ataagaaata agtctaatcg
    30421 gttctggaga atctactttt ccaatataaa gaacttcatt attgtcaagt ataccaagct
    30481 gacaagtttc agaacaggaa ttaactatat tagtcatttc gtcaattata tagttaaaaa
    30541 tagttctttt ttcaaaaaat gaagacccta atgtgtaaga acgtatccct atagaatatt
    30601 tggatgtgtt tttatcaata gtaatgtatt ttctagcatt aagtgtatga actataggaa
    30661 agatgctact ttttggagca ttgataacat ctgctatttc tgtaagagta tatccatttt
    30721 cggattctgc taatgcttct aaaatatcta atatcctagc tgttgggcga tgattatctg
    30781 aagtcataaa tttccccctc tttttattaa tttaaagtat tagaatatgt tgattttagc
    30841 acactatacg atattttaca atgtagaaaa taaatatgta aatttatata taaataagct
    30901 cttgatttag atacagatag atattttttt agactgtaca ttaaggaata actaatttcc
    30961 aaaatatacc taaagaaagt aagtgatatt tagttatata atttaagtaa atatatgcaa
    31021 tagttatttt tatatgtaaa tatcaaaata ttatgaactt atattttatc tattttttac
    31081 actgtataaa aactttatag ttttgaatac tatataattt taaatacgct gtatttttgc
    31141 atataaggaa gtcatttgat aatatacaag gtattatcag aaagtttgaa aaaattttga
    31201 caaaaattta ttttttttat tgactcataa ataggaaaat gctatcatta attcaaagat
    31261 tcgtaattaa aaacttagtt cttaaatacg aaccgaaacg gccgttagaa ttaaagctaa
    31321 aaacttaata agagtaatta aatatcatgt tttaggagga ttaactttat gataatggat
    31381 tttattaaaa aaattcctgc aggtatgatg atagtgccaa tgtttatagc agctttcatt
    31441 aatacctttt gcccagatat tgtacaaata ggttcgttta cgacagcagt attttcaagt
    31501 gttgggtctg caactataat tggggttcaa ctggtatgct tgggaactca gcttcaattt
    31561 agagaaatgc caaaagtttt aaagagaggt ggtatacttc ttggttctaa atttgctata
    31621 ggtgctttga taggtatact tataggaaag ctatttggaa tggatggtgt ccttggtctt
    31681 acaacattgg ctgttataag tgcagttaca aatagtaatg gtagtatata tctttcattg
    31741 atgacttcat atggagatga aacagattgt gcaactatga gtttacttac tttaaatgat
    31801 ggaccatttt ttacattaat agctcttggg acttcaggtc ttgcaaatgt accattgttg
    31861 tcattattgg ctgcaatagt gccaatactt gttggtatga taataggaaa ttttgacaaa
    31921 aaaatgaaag agtttttaga gccagggtgt aatttactcg taccttttgt agggtttgca
    31981 cttggtagta gcataaatct tgcaagtata ctaaaaggag gtttatcagg agtattactt
    32041 ggattaatat ctgtatttgt aggaggtata tttatagtat tctgtgataa atttatagga
    32101 aaacgtccag gatatgcagg atgggctgta gctacaactg ctggaaatgc aattgctgta
    32161 cctgctgctg ttgcattagt tgacccatca tggcaaccat atgttgcaac agctacaaca
    32221 caagtggcag ccgcagtagt tgtcactgca atattagtac ctcttataac tagttggtgg
    32281 gctaagaagt atggatgtcc taagttccca aaacaaaatg aggatggtac aataacagct
    32341 taatttatat tacagtgagt tgttggtaaa ataataaatt ctttaattag attttaggat
    32401 attaataaaa ttatattatt tataaaggag agcaagaata atgataaatg cagtgataat
    32461 tgatgaaaaa gataatgttg cagtagctat tgaagcaata gctaaaggta gtgaaattag
    32521 ttttaaatta aaagacaaaa cagtaaaaac gattacagca ctagatgata taactattta
    32581 tcataaattg gctataaaag atatggccaa aggtgataaa gttactaaat atggtgaaca
    32641 tataggagaa gcaaatcagg agataaaaag tggtcaacat gtacatattc ataatgttag
    32701 aagtgttaga gaggatttgg ataaacaagt tatgactaat atataaaata atatttatag
    32761 agttataaaa gttgtattca ttgcttttag tgtcaaatag tttttaaata ggtttttagc
    32821 tgtttgatta gtgagtgtaa taaaaattat aatatataaa aattatagaa agtatataga
    32881 ctattttagg aggaaatcag tatgaaattt tatggatata aaagaccaga tggaagagtt
    32941 ggtataagaa accacatatt aatattgcct acgagtgtgt gtgcatcaga tacaactaga
    33001 ataatagcat cacaagttca aggggcagtt acctttaata atcagaatgg atgttcacaa
    33061 gtacattcag accaacaatt aactatggat gttatggctg gttatgcagc aaatccaaat
    33121 gtatatggtg taatagttgt atctttggga tgcgaaaatt gccaaaatga tttggtagtt
    33181 gatgcaataa aagagagaac aaataagcca ataaaaactc ttgtaataca agaagaacat
    33241 ggaactttaa aaactataga aaaagctgtt cgttatgcta gagaaatggc acaagaagct
    33301 tctttgctta gaaaagaaga atttccaata tctgaactta tactaggaac tgaatgtggt
    33361 gggtctgacc caacaagtgg attagcagca aatccattaa taggtgaatt aagtgataaa
    33421 cttgtcgatt taggagcaac atcaatttta agtgaaacta ctgaatttat aggtgcagag
    33481 catatactag ctaggagagc agtaaatgaa gaagtaaaag aaaaaatatt acatatagta
    33541 catagatatg aaaattcatt aaaattggtt ggagaagaag ttagagaagg taatccatct
    33601 cctggaaata tagctggtgg tttaacaagt ttagaagaaa aatcacttgg ttgtatacat
    33661 aaaggtggtc atagacaaat aagtgaagta tatgattatg ccaaacaaat tgataagaaa
    33721 ggtcttgtta taatggatac ccctggaaat gatgcttcat ctgttgctgg tatggtagca
    33781 ggaggagcac aaatagtggt atttagtaca ggaagaggaa caccatcagg aaatccaata
    33841 gcaccagtaa taaaaataac tggaaataaa ataacttttg caaatatgga agataatatg
    33901 gattttgatg ctagtccagt tatctatggt ccacaaacaa tggaagaatt aacagatgat
    33961 ttgttaaata tggttgtaga tgtagcaaat ggaaaacaat caaaagcaga gtcccttgga
    34021 tatactgaaa tggcaatagc tagagtatgc aactatgtat aatctttaag taagaaaata
    34081 aatacataga attttaataa agggtgagat tatgaagttt gtaagtttta ctgaaggaga
    34141 aaatgaatgt aaaagaactg gagttttttc taaagatgaa ttatttatta tagatgttaa
    34201 ctctttaaat ctcagtagaa attttaagga tttaaatgaa ttaattcaaa atgtatctag
    34261 tgaagatatt attaagttga aaactattat aaatgaaagt acattctata attatgatgt
    34321 ttttaaaaga gagagatgta cggtacatgt tcctatagaa aaaccaatac atgatatctt
    34381 atgtgtggga gtgaactata aagaccatct tgaagaaact caaactcact ttgatgatag
    34441 ttttgaagaa ccacaaaaaa cggtttattt tacaaaacgt gtgacaaagg caataggtcc
    34501 agaagatgag atagatggtc attttgagat aaataatcaa ctagactatg aagttgaact
    34561 tggagtagta ataggtaaaa caggtgttaa tattagaaaa gaagaggtag aagattatat
    34621 ctttggttac actgttataa atgatatttc tgctagaaag ttgcaaaaag aacatgttca
    34681 atggtttaga ggaaaaggat tagatacatt tacctcttta ggtccatgta ttttacataa
    34741 aagtgcagtt ccttttccca taaaacttaa agtatgtagt agggtaaatg gtgaagaaag
    34801 acaaagttct aatacaggat tattcatatc agacgtttca gacatagttt cagaactttc
    34861 aaaaggtatg actttagagg ctggagacat tatagctact ggaacacctt ctggagtagg
    34921 gatgggattt tcacctcctc gatatatgag tagtggagat gtagttgaat gtgagattga
    34981 gagtattgga attttaaaaa attacattaa gtaaatgttt taatataatt aatattaaag
    35041 ctaaatctga tgtattaatg aacatattaa aataaatttt aggagtttaa attatgaaag
    35101 cagaattttt gacaccagtt gttacaatat ttgatgaaga aggaaattta gataaagaag
    35161 gtaataaaaa agtgtatgac catcttatag atggaggtgt agatggacta gtcataatgg
    35221 gaagtactgg agagttcttt aatatgtcta tggatatgca aaaagaactt atagatttag
    35281 taactagtca tgtaaataag agagtaaaag tgtttatagg tacaagtcgt atgtgtataa
    35341 atgaatctat agaactttca aactatgcac ataatagtgg agcagatggt gttatgataa
    35401 taagccctta ttatttctct cttgatgatg atagtattga gttattttat agtgaaatag
    35461 ctaagaatac agaggctgaa atatatttgt acaattatcc agatagaact ggttatgatt
    35521 taagtccaga acttacatta agacttgcaa gaaaacataa aaacatagtt ggatataagg
    35581 atacagtaac tttgatggga cataccagag atttaataaa tactatgaaa atagagttcc
    35641 cagaatttaa ggtttatagt ggttatgatg aaaactttat acataatcta atgtctgggg
    35701 gagcaggttg tatagggggg ttatcaaatt taattccaga agtttgctcc aaatgggctg
    35761 aagcttacaa taatagagat atggagaagg ttatagaaat tcaaaaatat attaataaag
    35821 caatggattt ttacttaata accaatccat ttataccagc aatgaaaaaa gctatgaata
    35881 tcagagggtt acaaataagt gattatatga ctgctccatt tgtaaaacca aatgaaaaac
    35941 aagtatgtga aattaaaaaa ttgatgaatg aattaaatat atgttaaatc aaccttgcaa
    36001 agaggtcaac aataaattac aattatctta ataaaaatta aattaaaata atactagggt
    36061 actatattag tttaataggg aaagtgatga aaattcacta cagcccccgc tactgtatac
    36121 ggatacgaaa tcaaaatcca ctgaaattta taaaaatttt gggaagggtg atggaggaaa
    36181 agccgtgagt caggagacct gcccagtatt atataagttc aatttagggt ggagattgta
    36241 atttataata aaatgaagta tcaattcgtt tttgtaaagt tgatatatga acttctcctt
    36301 attaaattag gagaagtttt ttattttatt aaaaggttat acttattaaa gatgacttta
    36361 ggataacttt aataagtata ctttaaaaag catatattat taaagttttt tagccactca
    36421 taaaaaataa gatattggag tgaaagagat gaaatttaaa tcaaatgttt taaagcagtt
    36481 ttttttaatt gctatggtaa tttttatggt aggaggtctt atagggtgta gtaaaagtga
    36541 caaagagaat aaacaagcta gtactggtaa gaaattagaa ttaaaatatg ctagtggatt
    36601 tagtgttgaa gatttagaag atggaattaa aaaagtaact gatggagata atcgagaact
    36661 gattttaatc ccaaagaaga ttaaaattcc tgaaaaatat aaaaatgcta atatagttag
    36721 aacgcctgtg gacaatgtat taatagcatc tacaacacag gcttgttctc tgagaacaat
    36781 agatgaacta gatagtataa aagccgtaac aacagaagag cagtactgga ctattaaaga
    36841 aattaaagat ttaatgaaag atggaaaaat acattacgtt ggttcaaatt tagcaccaga
    36901 ttatgaaaaa atagtagact taaatcctga cttaactata gtgagtagtg gtttatcaga
    36961 ggctgataca aaatttattg acaaattaaa agaattaggg cttaattatg ttgtagacaa
    37021 tgagtataaa gagcaaaatc catttggaag aatggagtgg acaagtttaa tagctgcatt
    37081 ttatgataaa gaagatatgg ctactagtga actaaataaa actgtacaaa aggtagaaga
    37141 agtaagtaaa aaaataaaaa acaaagctaa acctaaagtt gtatgggcat ctgtatatga
    37201 aggaagtgct tatatagctc ctaaagattc atatgtggat aatatgataa aaatggctgg
    37261 tggaataaat gcatgtgctt caatagattc aaatgaaggg cgagtaagta ttgaacaatt
    37321 gcatgaagtt gcaaaagatg cagatatatt tgtatactca tcaagttcag attatgcacc
    37381 aaatttagat tctataaaat ctagtgcacc tgtattggct gatttggatg ttgtaaaaaa
    37441 cgggaaccta tgggtgtttg ctccagatta ttatcaatca gtagataaga cggacgaatt
    37501 aatagtagat ttagttgaga tgttccaccc aggtactaca ggtgaaaaaa tcaaacatta
    37561 tataaattat gataaatagt acagagagga cacaaaaaga taggttttat agattttagt
    37621 taatatagct ttgctaagag gagtatacaa atggatttta tcaataaaat gagaataagg
    37681 aatacaacaa tatttatagt attaattata agtatgatac ttactttttt agtgtgtgta
    37741 gcatttggct ctgtaaaaat tgaatttaaa gaagtaatag atgttgtatt aagaggtatg
    37801 gataatacag aacattataa aataatatac aatataagaa tccctagagt acttgcaacc
    37861 atggtaggtg gagcatgcct agcagtatca ggtattttat tacagatatt ttttaaaaac
    37921 ccaatagttg aaccgtatat attaggagta tcttcaggtt ctactttatc agtagctctc
    37981 atgattttgg gaggatacac attgggattt gagagtgtaa attcaatgag tatatttgta
    38041 gcagctttta taggagcttt aattgtaatg tctatagtca ttttatttgc aaataaggta
    38101 aagaatataa caactctttt aataatagga attatgaccg gatatgtatg tagtggtata
    38161 acaaatgtgt tacaatcatt tgcaaatagt gataaattaa aagaatttac tatgtggtct
    38221 atgggaagtt tttctggatt tacatgggaa aaggtagaaa tacttataat agttggtata
    38281 ttaggtcttt tgatgactgt attcataata aaacctttaa atgctttttt actaggagaa
    38341 gagtatgcaa aaagtattgg cataaatata agatttttta gagttttgat agtatttata
    38401 tctagtatat tagcagcaat agtatcagca tttgcaggac ctatagggtt tattggactt
    38461 gcagtgccac aaatttcaaa aatgatattt aaaacatcag ataatagagt tttgatacca
    38521 gtagtaatat ttttaggcag tatagttaca gctctttgtg atttaatatc aagagtaatt
    38581 ttatcaccag tagaattacc aataagtacg ataacatctt tattaggagc tccaattgta
    38641 gtaatgttat tggtgaaaag gaaggatagg gcttagacat gttgaaaact aataatttaa
    38701 gtgtaggata caataacaaa gttgttataa gtaatataaa tgtagaagta aaaaatggag
    38761 aaatactatg tcttttggga agtaatggag ctggaaaaac taccctatta aggtccttat
    38821 ctaaacttat atcaccaata aaaggtgaaa tatatttgaa tggtgtaaac ataaattgta
    38881 tatcaagaaa agcattatca aaaaaaatgg ctttagtact tacaaataga ttattaggtg
    38941 atttaatgac tgtacaagat atagtcaata taggaaggta tccatataca ggattttttg
    39001 ggagtctatc taagaaagat ttaataatgg tagatgaagc attggagtct gtagatgcct
    39061 tacatttaaa gaaaagatat tttgatgaac taagtgatgg agaaaaacaa aaggtgttgg
    39121 ttgcaagggc tcttgttcaa gaaccagaaa taattatatt agatgagcct acaactcact
    39181 tggatataaa acatagattg gaactcataa acatactgaa aaaattaagt aaagaaaagt
    39241 caatatcagt aatactttca ttgcatgaaa tagacatagc attaaaaagt tgtgataaag
    39301 ttgctcttat aaaaaataat aaagtcatag cttatggtca gcctgaagat gtagtagatg
    39361 aggatattat aaattcgctt tatgaattgg atgataaaaa ctttaatagt cttttaggtt
    39421 cagtagaaat tagtaataaa tctaaaaatg aagtttttat aattggtggt gggggtaaag
    39481 caacaccaat ttatagagct tttactaaaa aaggtatagg tctgtattct ggaataatac
    39541 atgaaaatga tattgattat gaaattggta gaactatggg gataaagatg tttacagaaa
    39601 atccatttga gcctataagt gatgagagtt tcgatttggc tattagaaat ttaaatgatt
    39661 ccaagattat aatagataca ggatttagtg taggagagac aaataagagg aatatagata
    39721 ttataaaaga ggctttaaaa ttggataaaa aagtttattc ttttagaaat agagatgaga
    39781 gtaagaagta ttatgacagt ttggatagta agatagaaca tatagataaa gtttctcaaa
    39841 taatcaatag tgcagatata aataatttat tataatttat gaaataaaat gtacttatgt
    39901 gctatagaaa aaagagtata gattataatt tttttgaaat tacaatctat actcttttgt
    39961 tattttgttt atgtatttat atatttataa aactttagca ttttatagat aaccaatagc
    40021 ttcctctcta gttaaaaaaa agtgaatccc tgtagtcgat tctttccatc tattttcatt
    40081 aaaatcttca gctacaatcg tttctcctac tctatatata aaattttcat cagcatatgc
    40141 acgagcactt ttaaaagatt ctttaccatc tatactagta attgacagta cttttgcctt
    40201 atcacatcta caagcgtttg atgtagcaga cactcttttt gcgtcgctgg gtattaataa
    40261 ttgtaccatt ctaaaattaa aacatttttt atagcctata aatgctcctg tttcaggaca
    40321 atacaattta aaaaaacggg tactttcatc tgatattact ccatttaaag tagcatgttc
    40381 taaatttgaa ttttctaaat tagctcctct aatatctgag ttagataagt cacaaaatct
    40441 aaaacttgca ccacttaagt ctgcattttt taatatagaa ttttttaatg aacaatccga
    40501 aagataagaa ttattaaaaa tagagttttc taaatctgct ttttctaaat ttaaattgat
    40561 aaggtcagac cacgaaaagt caatattact taagcagtat cctcttaagt ccatattttc
    40621 taatcttaat tcttttaaat ctgccttttc tccagcatta ttgtttttaa gccataaata
    40681 atgtttctca agtgtttttt ctaattcttg tttagaaata gttttcatta aaatcacctt
    40741 cataaattta tttttatata tgtagtagca aaatagtcat atatttaatt atataacaat
    40801 aaataaaaaa cttctaaata atatataaaa tatcatcttg aaagtacttt tttcaatata
    40861 tcagaatata tttttaagct atttagagat aatttaaatt tttaaacagg acataataag
    40921 cttgtattgt tttatatttg agttgataaa aatttcttta aataaaaata atgtcaatta
    40981 taaagtaata aaagtttaga tataaaaaaa tttgtgtata tactatatgt agtatataat
    41041 agataaagca ctgactatat gtagtgcttt tgtttttgaa aaatacttat tgcatttcta
    41101 taattataga ttaattttgg gatttcaact gataagtacc aaaagaaata ataagcaaat
    41161 taagtgaaat tatggcacaa gattatgtta aaaggacggt aaataatgaa ttggatagag
    41221 cttaacaatc aagtgataat taaaaatgaa caaggcaagt accaattgga aaaggataag
    41281 gaagccttaa gtagttacat agaagcattt atacagccaa agttgagaaa atttaataac
    41341 ctagaagaaa gattgaagta tttaatagat gaaggttatt attcaaaaga agtaataaat
    41401 cagtatagca tagatgttat taaaaatata tatgatatga ttgataaaca taagtttgaa
    41461 tttcaatcat atatgagtgc aaataagttt tatcaaaatt atgcattaaa gagcaatgat
    41521 ggaaaggaaa tattagaaac atataatgat aaagtgttaa ttgtagcttt gacactagga
    41581 aatggcgata aagatttagc tttaaacttg gcagataaaa tagtaaaaca agaatttcaa
    41641 ccagctacac ctactttttt aaatgctgga agaaaaaggg ctggagaaat ggtatcatgt
    41701 tttttactat ctgtagaaga tagtacagaa ggtatatcgt atgctatatc ttcatcaaat
    41761 cacttatcaa agattggtgg aggagttgca ctaaatttat ctaaacttag agcatctggc
    41821 gaatctatca aagatataga aggtgcagct ggaggtgttg ttggagtagc taaaatgtta
    41881 gaacaatcat ttagttactt taatcaaatg ggagctagac aaggttctgg tgcggtgtac
    41941 cttactgtat tccatcctga ttttgaattg ttgatggata ctaaaaagat aaatgcagat
    42001 gaaaaaataa ggcttgctac attatcatta ggagctataa taccagataa atttatggag
    42061 ttggcagaaa aaaatgaagt agcatatgca ttttatcctc atactgtgta taagaaatat
    42121 ggagtttctc ttgatgagat agaaatggat gagtggtatg ataaattggt taatgatagt
    42181 gatataagaa aaaaagaaat aaatcctagg cagatgttga caaaaatagc acagatgcaa
    42241 caagaaagtg gatatccata tgtagtgtac atagatacag ctaatagaga acatacatta
    42301 aaagatgttg gaattattaa aatgtctaac ttatgttgtg agatatttca atatcaaaca
    42361 ccttcagaaa tagagggata tggaggaaaa aatgaatggg gacaagatat aagttgtaac
    42421 ctaggttctt taaacatagc caatgtaatg gataataaaa ctatagaaag tacagttgaa
    42481 acagcaataa gagcactgtc atttgtagca gatagcacag atataaaacc agtgccaacg
    42541 gtaagcaata gtaacagcaa gtctcattct ataggattag gagctatgaa tttacatgga
    42601 tacttagtaa gagaaaatat tttatatact tcagatgatg ctatagattt ttctaatgta
    42661 ttttttgcaa tggttagata ctattcaatt aaagcttcta tgaaaatagc tatagaaaga
    42721 aatcaaactt ttgaaggatt tgataaatca gaatatgtta agggaagaaa tagcaaggtt
    42781 ttatcaaaat actatgaaca atcttatcta cctaagtcag aaaaggtaag agcattattt
    42841 gaagggattt atataccaac aaaagaagac tggacaaaat tattggatga ggtaaaagaa
    42901 aaagggatat ataatgcata tttaatggca gtagcaccaa ctcaaagcat tagttatgtt
    42961 caaaatgcta catcaagtat aatgccaatt actgagccag tagaagtaag aacatatgga
    43021 gactctacta caatatatcc aatgccattt ttaacaaatg ataatatgtt gtattatcaa
    43081 tcagcataca ggatggatat gagaaaggtc atagatttag tggcaacagt tcaaaaccat
    43141 gttgaccaag gtatatcaac aacactgttt gttacagatg aaaagacaac tagagatata
    43201 gctagacatt acatatatgc ctataaaaaa ggtttgaaaa gtttatatta cacaagaaca
    43261 aagatgacaa gagatactca tgagtgtcta gtgtgttcag tatagggaga gaaatatatg
    43321 acatttaatt tacataaaat acataatgca gttaactgga atagagaaga agatggattt
    43381 acacaagctt tttgggaaca aaatgtaaaa caattttggc ttcctgaaga aatatctgtt
    43441 tctaaagaca taaaggtatg gaatgaacta agtaataaag aaaaggaatt atataaaaaa
    43501 gtattgggtg gtttaacatt actggataca aaacagggaa ataatggaat accatctatg
    43561 atgagtttaa cagaaaacct acaaagaaaa gctgtcttgt cttttatggg gactatggaa
    43621 gaaatacatg caaaaagtta ttcttctata tttatgactc tactttctaa tttagaaata
    43681 gatgaattat ttgaatggat agaaacagaa ccaacattac aaagaaaggc tgatttagtt
    43741 ttagctcaat acgaaaatac tacaaatcaa gagggattat acctatcaat ggttacaagt
    43801 gtatttttag agagtttctt gttttattca ggattctttt atccattgta tctatctggt
    43861 caaggaaaga tggtagctag tggagaaata atatcgctta ttcttagaga tgagtcttta
    43921 catggtaagt atataggatt gctagcacaa gaaatatacg attcatttga taaaatggat
    43981 aaggagatgt tagaagagaa gatgtattct atactttata gccttatgga gaatgagata
    44041 gagtatacaa atgttatata cagagaaagt gggcttgaaa aagaggttgt aaacttttta
    44101 aaatacaatg cgaatagagc tttagaaaat ctagggtttg agaagctata tactgtagat
    44161 gctataaatc caattgtatt aaatggtctt agcactgaaa ccaaaacaca tgatttcttt
    44221 tcaactaagg gtaatggata ccaaaaagga gtgtatgagg aattagagga tgaagatttt
    44281 ataatataat ttataattat gacacactgt tgtcactttt agcgtgataa tatcaatgta
    44341 taaaatatat atggaggtat ttttatgaaa tatgaatgga gaaaaagaga taaagagtta
    44401 tatttaccta aaaagaatcc tgtaatagtt gaaataccag aacagaaatt tatcatgtta
    44461 aaagggagtg gagacccaaa ttctaaagaa tttactgaag caataggagt actttattct
    44521 ttggcatatg caataaaaac catgcctaaa aaaggaattg taccagaagg gtattttgac
    44581 tatacggttt ttcctttaga aggtatatgg gataagggag aaaaaagtaa aggcttagat
    44641 gtacttatta aagaagattt aatttacact ataatgatac gtcagccaga ttttgttaca
    44701 caagacctgt tggataaagc tactgatata gtaaagaaga aaaaacctca taatcttctt
    44761 gaaaaagtta gatttgggtc tattaatgaa gggctatgtg ttcaaatgct tcatataggt
    44821 tcatatgata atgaaccaga aagctttgac attatgaacg aattttgcaa aaaaaataaa
    44881 cttataagaa aatcttacac acatcgtgaa atttatataa gtgatgctag aaaagtatcg
    44941 cctgaatcat tgaagacggt tttacgtttt gaagtagaaa gggaagtata aatattttga
    45001 ataatttata agagttataa aaagtgaagt ctgttgagac ttcatttttt gatgcaattt
    45061 taaaattaaa tttgatgttt cataatattt taaaaacttt ctacttttat tagtgtgaat
    45121 aactgttgct attaaataat a
//
LOCUS       DBGJFF010000017        43905 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.017, whole genome shotgun sequence.
ACCESSION   DBGJFF010000017 DBGJFF010000000
VERSION     DBGJFF010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 43905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43905
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.017"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(255..860)
                     /locus_tag="ACOIAF_000997"
     CDS             complement(255..860)
                     /locus_tag="ACOIAF_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HGZ2668018.1"
                     /translation="MQNTAKRGNMISTRTLVVMSILSAISYLLMFIQAPIPGIFPDFL
                     KIDLSDIPAIFGGMSLGPLVGFGIVVVKNVLQAITATTTGGIGEFANILIGGSYVMIL
                     CYSYKRSGDLKGVLIGFVLGTISMTIIGSLVNYFVMLPLYGQLMGLDAIIGLGSAINP
                     QVHDLFTFVIWMIAPFNVLKAVIISLVTLPLYKKMGNIIKK"
     regulatory      complement(985..1102)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(1162..2328)
                     /locus_tag="ACOIAF_000998"
     CDS             complement(1162..2328)
                     /locus_tag="ACOIAF_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HGZ2668019.1"
                     /translation="MIFIKNGKINTITNGIIHGDILIDEGKIIEIGEDLIAPLDVEVI
                     DASNKLVFPGFIDAHTHLGLWEDGIGFEGADGNEETDPITPQLNPIDGINPMDRTFKE
                     AFEGGITSVCTTPGSANVMGGQCIAIKTCGKRIDKMVIKNPVASKIAFGENPKSCYGQ
                     DDKSPQTRMAIAALLRENLKKAEEYLEDIDMYESHDDEDCEKPEYDIKMESLIPVLRR
                     EIPFKAHAHRADDMFTAIRIAKEFNLKLTLDHCTEGHLIVDELVEEGFPVIVGPSLSE
                     RSKFELRNLTFNTAGILSNAGLDVCIMTDHPVIPVQYLPICAGIAVKHGMKEEKAIES
                     ITINPAKTLGIEDRVGSIEVGKDADLVIWDNSPLEIQSNVLYTIINGKVVYEKK"
     gene            complement(2476..4617)
                     /locus_tag="ACOIAF_000999"
     CDS             complement(2476..4617)
                     /locus_tag="ACOIAF_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HGZ2668020.1"
                     /translation="MDINQILKKEFNLRDEQINNTLKLIDEGNTIPFIARYRKEMTGE
                     MSDVTLREFYEKLMYLRNLQSRKDDVVRLIDEQGKLTDEITQNIEKAKTLQEVEDIYA
                     PYKQKKRTRATIAKEKGLENLALSILENNLDNIEIEAKNYLDEEKEVLSIEDALKGAR
                     DIIAELVSDDAKIRKYIRELALREGMIVSKSATDEKSVYDMYYDYSEAVKSMAPHRVL
                     AINRGEKESFLKVKLEINNDKVLNYIINEYVNDKNFKNKEEIVSSIEDSYKRLIFPSI
                     EREIRNHLTEIAQERAISVFGKNVKSLLLQPPVKDKVVMGFDPAFRTGCKIAVVDKNG
                     KLLDYTTVYPTDPQNDVEGAKKVLKGLIEKYDIDIISIGNGTASRESETFVSEMIKEI
                     DSEVQYVIVSEAGASVYSASELANEEHPDINVSIRGAISIARRLQDPLAELVKIDPKS
                     IGVGQYQHDLNKKRLEEVLDGVVEDSVNSVGVDLNTASYSLLEHVAGISKAIAKNIIA
                     YREENGDFTSRAQLKKVKRLGPQAFTQCAGFMRILEGKNPLDNTGVHPESYDICKKMI
                     EIIGYSLDDVKNKNIGEIDEKIKEIGLRELSEKLEVGQVTLKDIIAEIKKPGRDPREE
                     GIKPILRTDVLKIEDIQEGMTLKGTIRNVVDFGAFVDIGIKNDGLVHKSEMSNSFVKD
                     PMSIVTVGDIVDVKVIGIDLNKKRVALSMKK"
     gene            complement(4761..5868)
                     /gene="prfB"
                     /locus_tag="ACOIAF_001000"
     CDS             complement(join(4761..5792,5794..5868))
                     /gene="prfB"
                     /locus_tag="ACOIAF_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_153309863.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HGZ2668021.1"
                     /translation="MLKLQEYKHSMEQMASNLEELRVSLDIASLMKNIQINEEKMNQQ
                     DFWNDNEVAQRVLQENKSLKETLEEYESLKSLLEDIEVLIEIGLEEDDDSVERDIEKS
                     IESMEEKLSEMKIKTLLNGEYDKNNAILSINAGTGGLDAQDWAQMLLRMYIRWSESKG
                     YKVKLLDIISDPEAGIKTATILVEGTNAYGYLKSEKGVHRLVRISPFDPSGKRHTSFV
                     SIDVTPELDENIEVEINPSDLKIDTYRASGAGGQHVNTTDSAVRITHIPTGVVVQCQN
                     ERSQHLNKDRAMRLLMAKLIELKELEQKEKIEDIQGKYSQITWGSQIRSYVFQPYKLV
                     KDHRTNAEFGNVDSVMNGNIDLFINEYLKMNKIV"
     gene            complement(5883..8558)
                     /gene="secA"
                     /locus_tag="ACOIAF_001001"
     CDS             complement(5883..8558)
                     /gene="secA"
                     /locus_tag="ACOIAF_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HGZ2668022.1"
                     /translation="MSFMDNLFNMADKKELKKFNKTVDIIDSLEPKFESMADSELKNM
                     TNIFKERLANGESIDDILPEAFAVVREVSKRVLGLRHYRVQMIGGIVLHQGRIAEMKT
                     GEGKTLVATAPVYLNALTGKGVHVVTVNDYLAKRDRDQMAKIYEFLGMSVGVIIHGQN
                     PKVRKEQYDCDITYGTNNEYGFDYLKDNMVIHKEQRVQRGLNYAIVDEVDSILIDEAR
                     TPLIISGPGDKSTHLYSDANTFVLTLKPDDYELEEKDKAVSLTASGIQKAEVYFNVDN
                     ITDISHTELYHHINQALRAHVIMKKDVDYVAKDGEIVIVDEFTGRLMFGRRYSEGLHQ
                     AIEAKEGLKIQRESKTLATVTFQNYFRMYKKLSGMTGTAKTEEEEFKAIYKMDVFQVP
                     TNKLMIREDLPDCVYKSEIGKFNAVAQEIIERHKVNQPILVGTVSIEKSELLSQILKK
                     KGIKHEVLNAKHHDKEAEIIAQAGRLGAVTIATNMAGRGTDIVLGGNPDFLTKREMRR
                     NGFKEEIVNRVDTPIEGIPVKGNEILFEAREEYEKLFEKFKQQTQEEQKQVVEAGGLA
                     IIGTERHESRRIDNQLRGRAGRQGDPGSSRFYIGLDDDLMRLFGSDRISGIVDKIGLE
                     EDMPIEHRILSKSIEGAQKKVEGKNFGIRKHVLQYDDVMNKQREIIYAERKRVLEGED
                     LQEQIQSMTHSIIEEAVTLYTQDKGFDEEGFKEHMYNLFLPKGSIEIPEIEKLNPVEI
                     TEKVYEIAMKIYTSKEEQVGYERMREVERVILLQAVDNHWIDHIDAMDQLRQGIGLRA
                     VGQQDPVIAYKMEGFDMFDEMNKHIKEDTVRYLFNITIETPVERKAVVDVENLSSPSD
                     GTLPTSKTVKKDEKVGRNDLCPCGSGKKYKNCCGR"
     gene            complement(8690..9241)
                     /gene="raiA"
                     /locus_tag="ACOIAF_001002"
     CDS             complement(8690..9241)
                     /gene="raiA"
                     /locus_tag="ACOIAF_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HGZ2668023.1"
                     /translation="MNIIISGKQIELTDAIKNKIETKLSKLDNYIHPDTDVKVTVSAR
                     KARQKIEVTIATVNGPIIRAEDMQENLYTAIDVVYDKLSKQLKKYKKRLQDKHKDNKS
                     IRFQDAEINLADEQEDYGDENQELVIQRRKKFSVKPMSEEEAVLQMELIGHDFYMFKN
                     IDSDEIAVVYKRNNGGYGIIEHE"
     gene            complement(9794..10468)
                     /locus_tag="ACOIAF_001003"
     CDS             complement(9794..10468)
                     /locus_tag="ACOIAF_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HGZ2668024.1"
                     /translation="MLEIIGMSVEDAKIIEDCGADRIELVSALTEGGLTPSFGLIESV
                     VNSVKIPVNIMIRHHAKSFVYSKEDISIMQKDIGVVKEIGANGVVFGVLDKNNNIDEK
                     NLNVLLKCCDNLDVTFHRAIDESNTIDSVKILKDYDKITNILTSGGKGSIVDNIQMIK
                     NMMLSSNHIKILLGGGLNFNNIEKIKELTKASNFHFGTAIRINNSPFEDIDRQKLKQL
                     VNIISI"
     gene            complement(10471..11523)
                     /locus_tag="ACOIAF_001004"
     CDS             complement(10471..11523)
                     /locus_tag="ACOIAF_001004"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HGZ2668025.1"
                     /translation="MNIISNKIDELKEDLLSDIIDIVKIPSVKGESENGFPFGEKVGE
                     ALNKALEISEKLGFKVRNLDNYIGYAEHGDSDDYVCVIGHVDVVHEGDGWKHQPYKGE
                     ETNGRIYGRGVLDNKGPIMSALYGLYAIKELNLKLDKRVRIIFGTNEESGFEDIPYYL
                     EKEKAPIMGFTPDCKYPVVYGEKGMAKIKIKSKINYEEDVYLGFIENMSENVLVTYKE
                     LNIENSDTILDIKVKYDFSYKLKDVLDEIKASFPNSIDIEVISNFNPVYFDKESNLVK
                     KLQLAYERVTSLDGTPVTTNGGTYAKVMPNIVPFGPSFPGQKGIAHNPDEYMDIEDII
                     LNAKIFANAIYELAKE"
     gene            complement(11572..12531)
                     /locus_tag="ACOIAF_001005"
     CDS             complement(11572..12531)
                     /locus_tag="ACOIAF_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HGZ2668026.1"
                     /translation="MWGMIATWRMALEGITEASEHLEKGMEAGDAVELAVRRVEDYPF
                     YKSVGYGGLPNENCEVELDAAFMDGNTLDIGAVAGIRDYSNPVSIARKLSYEKVNNFL
                     VGIGAEDYAHKNGFERKNMLTDRAKLHYKKRKKETLDKGLSPYAGHDTVGMISLDKTG
                     KMCAATSTSGLFMKKRGRIGDSALSGSGFYVDSSIGGATATGLGEDLMKGCISYEIVR
                     LMKEGKHPQEACDIAVNELDKQLIERRGKAGDLSLVAMNNKGEWGVATNIADFTFSVV
                     TKEESPTVYISNRVDGKTIHKVISNEWMEAYEARIKAPIEVSE"
     gene            complement(12627..13145)
                     /locus_tag="ACOIAF_001006"
     CDS             complement(12627..13145)
                     /locus_tag="ACOIAF_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrdB-related putative oxidoreductase"
                     /protein_id="HGZ2668027.1"
                     /translation="MKKIVMILDQIQAGAGGKEKSNIPPAGKSSPLGPGVMMEQFLNE
                     SKVIATLFCGDEFFVNNQEEVTSKMIAMVKKLNPDVVICGPSFNYENFSKMSAILSKN
                     INDKTDIPAFAAMSEENIDVINEYKNDICIVKTPKKGGIGLNDSLNNICKLAKAIANK
                     EDITLMKEEFCY"
     gene            complement(13156..14490)
                     /gene="celB"
                     /locus_tag="ACOIAF_001007"
     CDS             complement(13156..14490)
                     /gene="celB"
                     /locus_tag="ACOIAF_001007"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HGZ2668028.1"
                     /translation="MNRLERILMPLAEKIGKNKLLIAIRDGFLVSSPLLIIGSLFLLV
                     ANFPIKGWNEFVGQIFGPDWAVKLQQPTVATFEVMTLLAVLGIGYSYAKQIDVDPIAS
                     AAVAIVAFFIITPFVIPYTPEGTESVYMVTGIPLGWMGSKGMFVGMITAITSVKLFAA
                     VVKKGWTLKMPDGVPPTVSKSFAALIPSAIVMVVFFLVKIAFEATPYGSIHEFIFKFL
                     QMPLLKLGNSLGAMAIAYIFLHLFWFFGINGSSVVGAVYNPILKILSAENLAAFQAGS
                     KIPNIITGQFQDMFATFGGAGSTLSLVIAMLLICKSKRIKSMGKLSILPGIFGINEPL
                     IFGLPIMLNPILLIPFAIVPTINIIIAYFCMSAGLVPLTNGVQLPWTTPIIFSGFLTT
                     GWQGAVLQLVLLILGIFMYIPFIKMLDKQYLREENETVEEEDDDISFDDLVL"
     gene            complement(14529..14855)
                     /locus_tag="ACOIAF_001008"
     CDS             complement(14529..14855)
                     /locus_tag="ACOIAF_001008"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGZ2668029.1"
                     /translation="MKRKVYLFCSFGMSTSLLADKMQKVADEHNLPIEVEAFPIAEID
                     KIVEEKNPDCILLGPQVKYMLKELKPKFEAQGKLIDIINEVDYGTMNGEKVLKLAIKL
                     IKSKQA"
     gene            complement(14879..15187)
                     /locus_tag="ACOIAF_001009"
     CDS             complement(14879..15187)
                     /locus_tag="ACOIAF_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HGZ2668030.1"
                     /translation="MEEIVLKIIIHAGNAKSMLYEALDYAKENDFKKADELIENANEE
                     ILKAHKVQTELIQKEAGGDKSDISILLIHSQDHLMTCMSERNLIKEMIMLRKEIQKLK
                     "
     gene            complement(15328..17241)
                     /locus_tag="ACOIAF_001010"
     CDS             complement(15328..17241)
                     /locus_tag="ACOIAF_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGZ2668031.1"
                     /translation="MICLNKRQENLLTTLLLKEEFINVNKIANDFECSERTIRNDCKY
                     IDEWLSTFCNAYIKRKPNIGIKFSGDGTDKILVNQKLRGEVKRVNSELHKQVEILNLL
                     LCNNKKVTLNDLSNKLYINKNIVREEISKLSVALNNYNLNISTKKGSGIYIEGDEKDI
                     RIMLVSFLFKYIDKYRLNIDEIEYFHIVDIIIVKNIMLTIEEDMDIRLTDISFKQLML
                     FLLINIIRIRLGNSLKVSNTKKELKLKMLILDIEDELKSNLSIVLNYEEKLFIESLIL
                     GMNKQINSNNIKKSLYLNEELASYTKKIINLVSEESGINFNNDILLYEQLICHLNVTM
                     HQMKSNVYLENPLLDNIKTKFCFLFTVISSSIEIELKGKQITEDEIGYLTLHFQTSLE
                     RKYSKRESNKKASIVCPFSFGVSMLLKVKIEKRFDNIKIIETLREEDLKRDNFNKTID
                     FIIAFQEYENIQKPIFITTPLFTDEDENKLKEFTNKIKEKDTSYKLMNRLMMSDYLIR
                     ELEHDDIYEAITYLTNLLIEKHYAEKEYLQSIITREKSYPTNVGKGILFPHGDMKYIK
                     QSVLCFARLKTPIKIRNGKDIKIILLLAYKKDDDRKEFRELFKEISNLTEDENMLDIL
                     SKCDIEKVKDILI"
     gene            complement(17573..18313)
                     /locus_tag="ACOIAF_001011"
     CDS             complement(17573..18313)
                     /locus_tag="ACOIAF_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HGZ2668032.1"
                     /translation="MINKCLDFIYPENISCIICDKSIKKTNTYSICKSCFKEMNFIQD
                     GCMKCGKPIIRHSIEKEFIEECSYCFNKDFYFDKSISCIEYNDVTKKMVLGLKYNQKT
                     FMAKYIAQIMKEKLYLENIKFDYILFVPLHKKRLNKRGFNQAQKIAFNLSKIVNIPLL
                     DCISRKKYTRMLYKLNKKERKEELKNVFVVKENVKLINNKNILLIDDIFTTGLTTNEI
                     SKLLKLSGANKVFVLTLLTKANDNYIME"
     gene            complement(18358..20577)
                     /locus_tag="ACOIAF_001012"
     CDS             complement(18358..20577)
                     /locus_tag="ACOIAF_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HGZ2668033.1"
                     /translation="MEKLEGMISEIVFKNEDNGYTIAHLVNENDEIVVVGCMPTLAIG
                     ESIEVEGKWVNHKIYGTQFEVNSFMPVTPSSLEGIYVYLSSGMIHGIGEKMAKRIIDK
                     FGVDTLEVIQNSPEKLQEVEGIGSKKVKQIVKSYEEDRELRNIIIQLSPFGITPNYCL
                     KIYKKYKSSAIEVINKNPYQLAEDIRGIGFKVADSIASKIGIDKNSKDRICQGILYTL
                     NKSLSNGHTYLPEHVLIQDSEKLLELNGEIIKECVMMLVYNQKIHIEKVNNENLIYLM
                     PYYLAENGVCSQIVKLSQYEFEDLKIDIDNEINVLEEDKKIKLAEKQILAVKESVNSG
                     VLIITGGPGTGKTTTINAIIDIFENNGKSVTLAAPTGRAAKRMSETSNKEAKTIHRLL
                     EMGFSTDDDLTFFKDEEDPINSDVIIVDEVSMVDIILMYNLLRAIKLGTRVILVGDSD
                     QLPSVGAGNVLKDMINSNIINVVKLNEIFRQAQESMIIVNAHKINNGEPLYLNTKGKD
                     FFFIRKSTNEEILNEIIGLVNERLPKFYKVDKLKDIQVLSSMRKGELGVTNLNIELQK
                     YLNKKEKFKVEESFSKRLFRVGDKVMQVKNNYTKKWETEDQKESGEGIYNGDIGYVYH
                     IDKDKKTIYILFDQTKIVSYLYDELDEIDHSFCTTIHKSQGSEFPVVVLPIAWAPPML
                     LSRNLLYTAVTRAKKLVVLVGDVKYLEYMIKNNRVNQRYSNLGYKLNKFKQEGLLIE"
     gene            complement(20841..21938)
                     /locus_tag="ACOIAF_001013"
     CDS             complement(20841..21938)
                     /locus_tag="ACOIAF_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668034.1"
                     /translation="MSGKVNIKTVISFAGAYVATVIGSGFATGQEILQFFTFYGYAGI
                     IGGIISMVLFSWFGAEVIDKGRELKLKEPIKIYQVYCGKYLGTFFEWFGPLFLFGTLV
                     IMIAGAGATLSEYYGLNPYVGRIGMAIVSLITVSLGLTRLSKILGNIGPIIIIFTLLV
                     GAISLFSNIDALSNAGNMVDSLNIKTATSNGYFSGVLYTCYNVIIVITFLTGMGASAV
                     NKKDAVWGGIVGGVALMAAAIMMNLALLSDIGNIYTKEIPALYLADKISPIIGILFSV
                     VLLLGIYTTAVPLLWSVTNRFVEDDHPKFKIITIVVSILACIGGLLPFDKLVGTLYPY
                     TGYMGILILLCMLYRRITKTEGYKENKSEIS"
     gene            complement(22254..23447)
                     /gene="metK"
                     /locus_tag="ACOIAF_001014"
     CDS             complement(22254..23447)
                     /gene="metK"
                     /locus_tag="ACOIAF_001014"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HGZ2668035.1"
                     /translation="MARHLFTSESVTEGHPDKICDQISDSILDALLEKDPQSRVACET
                     TVTTGLVLVAGEISTSAYVDIPKLVRETVREIGYTRAKYGFDCDTCAVITSIDEQSGD
                     IAMGVDEGLESKTGEEIEEEIEKVGAGDQGIMFGFACNETPELMPLPISLAHKLSRRL
                     TEVRKTGLVDYLRPDGKTQVTVEYEGSKAVRVHTVLISAQHCETVSNDKIREDLINHV
                     IKEVIPAELLDEETKIYINPTGRFVIGGPQGDTGLTGRKIIIDTYGGYSRHGGGAFSG
                     KDPTKVDRSAAYAARYVAKNIVAAGLADKCEIELAYAIGIARPLSIFIDTFGTGKVSE
                     EKLVELVNKHFDLRPGAIIRDLDLRKPLYKKVAAYGHFGRTDIDLPWERTDKVEQLRK
                     DALGE"
     regulatory      complement(23584..23687)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            23876..24385
                     /gene="yyaC"
                     /locus_tag="ACOIAF_001015"
     CDS             23876..24385
                     /gene="yyaC"
                     /locus_tag="ACOIAF_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protease YyaC"
                     /protein_id="HGZ2668036.1"
                     /translation="MYLAKVNYSDDDVIQMLSSVLKTIINENTIVVCIGTDRAIGDTL
                     GPLVGTILKNSNFKYPVYGTLDNPIHALNIYESLDTIKNTHIQGNFLAIDACLGSQSN
                     IGNIQIREGPILPGKGVGKKLPQIGNYSIVGIVDKIDENNKLSFNNIRLSFILDLAET
                     IALALLVST"
     gene            complement(24496..24924)
                     /gene="fabZ"
                     /locus_tag="ACOIAF_001016"
     CDS             complement(24496..24924)
                     /gene="fabZ"
                     /locus_tag="ACOIAF_001016"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HGZ2668037.1"
                     /translation="MLNIDEIKKLIPHRYPFLLVDKITELEVGKRAVGIKNVTVNEPF
                     FQGHFPEYPLMPGVLIVEALAQVCGVAMMSVEENKGKLGVFAGIDKVRIKREVRPGDT
                     LTMEVEMTTLRKNIAKADAKAYVGEELVCKGELMFALVEK"
     gene            complement(24940..25959)
                     /locus_tag="ACOIAF_001017"
     CDS             complement(24940..25959)
                     /locus_tag="ACOIAF_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HGZ2668038.1"
                     /translation="MAGADIGIDLGTANVLVYVSGKGIVLEEPSVVAIDKRTDSVLAV
                     GEEAKKMIGRTPGNIVAIRPLRDGVISDYDVTEKMLKSFIDKIVDKKGFGRFFMPRIM
                     VCVPTGVTEVEKRAVEDATRQAGAREVYIIEEPIAAAIGAGVDISQPNGNMIIDIGGG
                     TVDIAVISLGGAVVSESIKVGGDRFDDAIVKYMKKQHNLLIGERTAEKIKFEIGSAFK
                     REEEKYMKITGRNLITGLPSSITINSTEMLEALRECVEQIVVATHAVLEKTPPELAAD
                     IGDVGIIMTGGGSLLYGLDKIIEQRTGITVTIADEPLSCVAKGTGIALGSIDLLETGG
                     SFKRK"
     gene            complement(26156..26416)
                     /gene="spoIIID"
                     /locus_tag="ACOIAF_001018"
     CDS             complement(26156..26416)
                     /gene="spoIIID"
                     /locus_tag="ACOIAF_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcriptional regulator SpoIIID"
                     /protein_id="HGZ2668039.1"
                     /translation="MRSHIEERAIVVAKYILEKNTTVRQTAKTFGVSKSTIHKDVTER
                     LKEINPSLAKEVKNVLDKNKSERHIRGGLATKLKYEKEHKKM"
     gene            complement(26514..27182)
                     /locus_tag="ACOIAF_001019"
     CDS             complement(26514..27182)
                     /locus_tag="ACOIAF_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HGZ2668040.1"
                     /translation="MKKKLLEKDGFYLSLFVCVCLLAVGGVWFTNNNVDKLASNKGIM
                     ENANKDSEEEIHLIEKDKKDAIPTATDSKQNLEKAKSKEENKSSTTKLNYIGDKVIRG
                     YSEKEPSYSKTLDVWETHKGVDISCTKGKEVKSLLNGIVVDVFDDEEYGQSVKIKSDN
                     NIVVVYSNLDKNVSVKKEQKVTEGQSIGTVGSTSQIESEEGIHVHLEAYSGEKSIDPM
                     SLIK"
     gene            complement(27248..28312)
                     /gene="spoIID"
                     /locus_tag="ACOIAF_001020"
     CDS             complement(27248..28312)
                     /gene="spoIID"
                     /locus_tag="ACOIAF_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein D"
                     /protein_id="HGZ2668041.1"
                     /translation="MKNPLVVLLGFVTCSVLVPSLITLVSYKNVELTEKPESPVSINK
                     TIKKSDIEDKGNKEEKNTVNYETVNKKAPIINVYNHITGKTEKMDMENYLCGVLAGEM
                     SSEFDIEALKAQSVAARTYVVYKQEHGKSSKHKNAVVCTDYKHCQEYKSYDTLKKLNG
                     EEWIKNKYSKIQEAVRGTKGQIITYNDKAILPLYFSTSSGKTENSEEVFSAKYPYLKS
                     VESPYDKYSPKFASTLKISNTDFVKSLRRAYSTIVIDVNNLSKQVSITKRSDAGTVEK
                     IKLGNKELTGKDIRTVFKLNSANFDIKFGEGYIDFVVKGYGHGVGMSQWGAEGMAEEG
                     YKYYDILSHYYTDTKIKDIY"
     gene            complement(28399..29652)
                     /gene="murA"
                     /locus_tag="ACOIAF_001021"
     CDS             complement(28399..29652)
                     /gene="murA"
                     /locus_tag="ACOIAF_001021"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HGZ2668042.1"
                     /translation="MAKIIVKKSNPLKGSVKIDGAKNAVLPIIAATLLANGKSTLNGV
                     PNLRDVHVISDLLRHVGAEVEYKENTLTVDASNIKTCEAPYELVRKMRASFLVMGPLL
                     ARFNSTKISMPGGCAIGTRPIDLHLKGFKALGAKIEMDHGFVEAATEKLVGNKLYLDF
                     PSVGATENIMMAASLAEGTTIIENAAEEPEIVDLANFLNEMGADVKGAGTNTIKIKGV
                     KELKGAEHNVIPDRIEAATYMVAAAMTKGDITVENVLMEHLKPVVAKLREAGCEITEM
                     DNSVRVVGPKVLKPIDIKTLPHPGFPTDVQAQFMAMLTVANGTGVVIETVFENRFMHV
                     AEFNRMGANIKIDGRSAVVNGVDELHGAAVNATDLRAGAALILCGLIAEGETQIGEIY
                     HIQRGYVDIDKKITALGGQIEIVED"
     gene            complement(29673..30410)
                     /locus_tag="ACOIAF_001022"
     CDS             complement(29673..30410)
                     /locus_tag="ACOIAF_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwmB family TATA-box binding protein"
                     /protein_id="HGZ2668043.1"
                     /translation="MKKVKLAISFFILMIIGMTISYADTSINTRYNRLSKALEISEAN
                     FKFYNVKINSVINHNLSNKEFHNIFIEIINNLKLDKKGINWNQKGSGDKIQLCATIKG
                     SDKEISIKGINKNNKESYIVVDILDNKVYKHKEVIYSNVENVLNKYSSQVDVSACIVG
                     EYTKRLQNNKYDDILQKILYNMSAKQIDEVKDVNFLSITAYSKVLNDYELEYLGEKIN
                     LNIGIKYNEDDGKTLIYIATPIIKLDY"
     gene            complement(30845..32140)
                     /locus_tag="ACOIAF_001023"
     CDS             complement(30845..32140)
                     /locus_tag="ACOIAF_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HGZ2668044.1"
                     /translation="MTNDFKKEKKEDYFVNLKAISTEVLQEKVQDNAWVIVDTRLNDA
                     YNGWKLDGVKRGGHIKGAVDFSANWLSVYSDRKDEVLEQALKTKRIDLDKNIVLYDAN
                     GKDALVVADYLSKKGYKYLYKYDIKQWADDENLPMERYKNYQMIVPAFIIKDILDGKI
                     PETFEDSKNIKMIEASWGEESYTKGHIPTSVHVNTDIIEPPPTWMLDNDDNLTKFALD
                     YGLTKDDTVIVSSSTPMASYRLAVILRYIGVKDVRVLNGGTNSWLSAGYELEFISNPK
                     HSCTNFGADIPVNSQLIVTTSELRQKLKEKNKFILVDNRTWDEHIGKVSGYTYYDKKG
                     RIPGALYGHSGSDSVSLEEYRNIDNTMRNKYEILEMWDKENIDVNKQLIFMCGSGWRA
                     AEVLTYANVIGVENTSLYSDGWMGWSLDNSNLIEVGEHK"
     gene            complement(32591..34423)
                     /gene="glmS"
                     /locus_tag="ACOIAF_001024"
     CDS             complement(32591..34423)
                     /gene="glmS"
                     /locus_tag="ACOIAF_001024"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HGZ2668045.1"
                     /translation="MCGIVGYLGSRKAAEVIVEGLSKLEYRGYDSAGVAVNSSNEKEL
                     NIRKFKGRLSVLAEDLEKNPIDGNLGIGHTRWATHGEPSDVNSHPHFNQAKTIAVVHN
                     GIIENYMEIKEELISEGVKFESQTDTEVIAHLVDKYYEGNLLDAVYKTISKLRGAYAL
                     GVICKEHGNELVAVRKDSPLVVGVGEGENFIASDIPALLKYTRDVYFLENGEVVHLKD
                     ENVTVYDSNRNLVEKEVFHVTWDVEAASKGGYDYFMSKEIHEQPTGVRETLERRLDDN
                     GNIILDSINISKEDLEKINKVYIVACGTAYNAGLLGKYAIEKFVNIPVITDIASEFRY
                     SDPFVDENSLVILVSQSGETADTLAVLRDSKAKGARILSITNVVGSSIARESDDVFYT
                     WAGPEVAVASTKAYTTQITSLYMIALDFAIKKGTITREFYDSMISKMKEIPSKIQEIL
                     DNEEYIKEVAKTVVSSEHAFYLGRGIDYSLAMEGSLKLKEISYIHAEAFAAGELKHGT
                     IALIEKGTPVIAIATQEKLFEKMVSNMEEVRARGAYVVAIAQSHNKDVEKAADKIIYI
                     PNSDDILSPILAVVPMQLLAYHVSVLRGCDVDKPRNLAKSVTVE"
     gene            complement(34690..36036)
                     /gene="glmM"
                     /locus_tag="ACOIAF_001025"
     CDS             complement(34690..36036)
                     /gene="glmM"
                     /locus_tag="ACOIAF_001025"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HGZ2668046.1"
                     /translation="MRKYFGTDGVRGVANTELTCDLAYKLGRAGGFVLAQGDHRVKVV
                     VGKDTRISGDMLEASLIAGLMSVGCDVITVGIIPTPAVAYLTRKYGADCGVVISASHN
                     PVEYNGIKFFNKNGYKLDDEIELKIEEYIDDIDKIDCLPIGENVGRKLHEHCAQRDYV
                     DYLKSIISTDFKGLKVVLDCANGASYKVAPIVFDELGASVISINSSPDGNNINYKCGS
                     THPEQLQRAVLEHNADLGLAYDGDADRLIAVNEKGQIVDGDHIMILSALNLKKNNKLA
                     QDTLVVTVMSNIGLTIAAKENGINLSTTAVGDRYVLEDMVKNGYNLGGEQSGHMIFLD
                     YNTTGDGVLSSLILANIILQEKKPLSEIASIMSQYPQVLVNATIKNENKNKYMEYPEI
                     KTEIERIESILDGNGRVLIRPSGTEPLVRVMLEGKEEGQIKELATNLANLIQEKLS"
     gene            complement(36206..36763)
                     /locus_tag="ACOIAF_001026"
     CDS             complement(36206..36763)
                     /locus_tag="ACOIAF_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HGZ2668047.1"
                     /translation="MSTARVICAGFGGQGVMSMGQLLTYAGMLEGKEVSWLPSYGPEM
                     RGGTANCAVTVSNEPVGSPLITDDATAAILLNIPAFEKFKDDVIPGGKIIVNSSLIKE
                     KVDRTDVDVYYIPANELAAELGNDKVANMIMLGAYLKVSDTVDIESVLEAFKKVFGPR
                     KEKFVPLNREALQKGMDATCSKATN"
     gene            complement(36764..37516)
                     /locus_tag="ACOIAF_001027"
     CDS             complement(36764..37516)
                     /locus_tag="ACOIAF_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HGZ2668048.1"
                     /translation="MAVVFKKTEGLQDTQTHYCPGCTHGIIHRLVGEVLEELGVLGDA
                     VGVVPVGCSVLGYKYFNCDTQEAAHGRAPAAATGIKRVHPENTVFTYQGDGDLASIGT
                     AEIVHAAARGEKITTIFVNNTTYGMTGGQMAPTTLVGQRATTAQSGRNAETQGYPIRV
                     SEMLATLTGAVFVERVAVDTPAHVRQAKKAIKKAFQVQQAGLGFGIVEVLSTCPTNWG
                     LAPNDALQWLRDNMIPYYPLGNFKNVEVEEVK"
     gene            complement(37516..38595)
                     /locus_tag="ACOIAF_001028"
     CDS             complement(37516..38595)
                     /locus_tag="ACOIAF_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HGZ2668049.1"
                     /translation="MAKILMKGNEAFGKAAIEAGCKYFFGYPITPQSELPEYLSRELP
                     KIGGAFVQAESEVSAINMVYGGAGAGARVMTSSSSPGVALKQEGITYAVGAEVPCVVL
                     NVMRGGPGLGSIQPSQADYFMSTRGGGNGDYRTPVFAPATVQEAVDMIMEAFDVADYY
                     RSPVMVVADGMIGQMMEPVEFRAPEKKRELPPKDWATVGTKGKRKPNVINSLYLEPEV
                     LEDHCWHLQEKFDAMEKNEVQYEMYKTEDAEFVFAAYGTTSRVVKSAIDILREEGIKA
                     GLIRPKVLWPFPFEAFNQIPNARNILTVEMSMGQMVEDVKMAVEGKLPVYFHGRPGGM
                     TPTPAEIVEKAKKIIAGELVAGGAR"
     gene            complement(38624..38839)
                     /locus_tag="ACOIAF_001029"
     CDS             complement(38624..38839)
                     /locus_tag="ACOIAF_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HGZ2668050.1"
                     /translation="MAKGKVSFNQERCKGCGLCVEACPVKIIQLDSNVINKKGYNPAT
                     VFEMEKCIGCASCATMCPDVVITVERD"
     gene            complement(38882..39559)
                     /locus_tag="ACOIAF_001030"
     CDS             complement(38882..39559)
                     /locus_tag="ACOIAF_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGZ2668051.1"
                     /translation="MITNDKRIRIITGHYGSGKSEFAMNYVVKLRDMVSGKVAIADLD
                     VVNVYFRTREKKELMKSLGIQPIDSSINAPTLDLPAVSAEVMSPMVDHSYNSVIDLGG
                     DNVGARVIGRFSHLLKEGDYDMLFVINANREKTQTSEEVIQYIKEIEKSSKLKVTGLI
                     NNTHLIRFTTIDDVLRGQKVAKEVSEKCNIPIRYVACLENLVEQLPKDLEGEIFPIKL
                     YMREDWM"
     gene            complement(39549..40628)
                     /gene="buk"
                     /locus_tag="ACOIAF_001031"
     CDS             complement(39549..40628)
                     /gene="buk"
                     /locus_tag="ACOIAF_001031"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HGZ2668052.1"
                     /translation="MSKIFKILTINPGSTSTKIAVFDNEDLVFEKTLRHSSEEIGKYE
                     KVSDQFEFRKQVIEEALKEGGVKTSELDAVVGRGGLLKPIKGGTYSVSAAMIEDLKVG
                     VLGEHASNLGGIIAKQIGEEVNVPSYIVDPVVVDELEDVARISGMPEISRASVVHALN
                     QKAIARRYAREINKKYEDINLIVAHMGGGVSVGAHKNGKIVDVANALDGEGPFSPERS
                     GGLPVGALVKMCFSGKYTQDEIKKKIKGNGGLVAYLNTNDAREVEERIEAGDEKAKLV
                     YEAMAYQISKEIGASAAVLKGDVKAILLTGGIAYSKMFTEMIADRVKFIADVKVYPGE
                     DEMIALAQGGLRVLTGEEEAQVYDN"
     gene            complement(40650..41552)
                     /gene="ptb"
                     /locus_tag="ACOIAF_001032"
     CDS             complement(40650..41552)
                     /gene="ptb"
                     /locus_tag="ACOIAF_001032"
                     /EC_number="2.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050182 - phosphate butyryltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019605 - butyrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate butyryltransferase"
                     /protein_id="HGZ2668053.1"
                     /translation="MRSFEEVIKFAKERGPKTISVACCQDKEVLMAVEMARKEKIANA
                     ILVGDIEKTKEIAKSIDMDIENYELIDIKDLAEASLKSVELVSQGKADMVMKGLVDTS
                     IILKAVLNKEVGLRTGNVLSHVAVFDVEGYDRLFFVTDAAMNLAPDTNTKKQIIENAC
                     TVAHSLDISEPKVAAICAKEKVNPKMKDTVEAKELEEMYERGEIKGCMVGGPFAIDNA
                     VSLEAAKHKGINHPVAGRADILLAPDIEGGNILYKALVFFSKSKNAGVIVGAKAPIIL
                     TSRADSEETKLNSIALGVLMAAKA"
     gene            complement(41856..43037)
                     /locus_tag="ACOIAF_001033"
     CDS             complement(41856..43037)
                     /locus_tag="ACOIAF_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HGZ2668054.1"
                     /translation="MINRLKNNTKIKIISFLSAIALWMYVMAVVDPEETKGFEDIPVS
                     ISNMSDLKDKNLVIYPDEKLTADVYIKGSLSKVQSVKKEDIHVYGTIEDPIQGQKEVY
                     LKANIPQGVTLEFKHDVLVVNLEKNIKEKRDIKVEVSGNSKRNINKIELSKEFVDVSG
                     PRTLVKEVKSVRANLDVGNETDDFSKKLQLIPLNSDGTKVEGVTLSSSFVTAKIELLK
                     EKIVPIRVSLNDNKDNDVLKNYKLSKEQVTIKGKKDDIDKISYIKTKPIDLADLANGE
                     EKEVSLEIPEGITLEDSSITIKLNDTKQLSAEFLYNAGDIELRNIPTNMDNPNVISDV
                     DIKVVVESTEDLSKLNKKDISLYIDLSQGPEEDSKYKIKYETTYQFKKINIEPNIVEV
                     E"
     gene            complement(43034..43873)
                     /gene="cdaA"
                     /locus_tag="ACOIAF_001034"
     CDS             complement(43034..43873)
                     /gene="cdaA"
                     /locus_tag="ACOIAF_001034"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HGZ2668055.1"
                     /translation="MLDINSFLNGFFNIILRMSIYDLIDISIVAYIFYKIFMFIKDTR
                     AEQVFKGIIFLLLATQLSNTFKLHTVYWISLKALDYGVIAALIIFQPEFRAGLEHIGR
                     AKFNLFGKNVNTSEETLNRNIEEIVEALYSLSRQKIGALIIMERETRISDIINTGTII
                     DAEISRQLLINIFIPNTPLHDGAVVIRDSKVKAAACFLPLTESKDLSKDLGTRHRAGI
                     GVSEVSDCITLIVSEETGGVSIAKAGKLYRDISRERMMNILRSNLKTNTETRSFFKGG
                     IFK"
BASE COUNT        13008 a         8387 c         4707 g        17803 t
ORIGIN      
        1 ataactttat tttttgttta ttataaattc gctctgtata ttgtagatac ctttttcata
       61 ttttaatagt taatcgtttt aattcttcct ttattttaaa atttaaattt gtctaaatat
      121 ttcttatttt atatacattt tttgataaat taataaagcc ataatatatt taatatatca
      181 atcacaatat aggactgaag ttataaataa attatggcta ttcaatacat ttaattttat
      241 attttcttgt ctaattattt ctttattata tttcccattt ttttataaag tggcaatgtt
      301 actaaagata ttataacagc cttcagcaca ttaaatggag ctatcatcca aattacaaaa
      361 gtgaataaat catgaacttg aggatttata gctgaaccta aacctattat agcatctagt
      421 cccataagtt gaccataaag tggcaacatt acaaaataat ttactaatga accaattata
      481 gtcatagata ttgttcctaa tacaaaacca atcagtacac cttttaaatc tccacttctc
      541 ttatatgaat aacaaagtat cattacataa gaaccaccaa ttaatatatt tgcaaattct
      601 cctattcctc cagttgttgt tgctgttatt gcttgtaaca cattttttac tacaactatt
      661 ccaaatccaa ctaatggtcc tagagacatt cctccgaata ttgctggaat atctgataag
      721 tcaattttta agaaatctgg aaatatacct ggtattggtg cttgaataaa cattaataaa
      781 taagatattg cagataaaat tgacattact actaaagttc ttgttgaaat catattgcct
      841 ctttttgctg tgttttgcat aactattttc cccctaatca atatgtttat attttttagg
      901 aaaaacaaaa aactcgaaga ccctatcttc gagtttttat ggatatagtt taaacaaact
      961 aaaataaact tatttatctt ccataaatat cttctctcat ccagacttta ctgtcggctt
     1021 tggaatctca ccaaatcaac cacctaagta gttcgcgggc tataccgccg gtagggaatt
     1081 tcaccctgcc ccgaagatct tctttgattt tttaattttc cttacactct tatattagtt
     1141 aattttttaa taattgtcaa gttatttttt ctcatacact actttgccat ttattatagt
     1201 gtaaagtaca ttactttgaa tctctagtgg agaattatcc caaattacta ggtctgcatc
     1261 tttaccaact tctatagagc ctactctatc ttctattcct aaagtcttag ctggatttat
     1321 cgtaattgat tctattgctt tttcttcttt cataccatgt tttactgcaa tacctgcaca
     1381 tattggaaga tattgaactg gtattactgg atggtctgtc attatacata catcaagacc
     1441 tgcatttgaa agtattcctg ctgtattaaa tgtcaaattt ctaagctcaa atttagacct
     1501 ttctgacaaa gatggcccta ctattacagg aaacccttct tccactaatt catctacaat
     1561 aagatgacct tctgtacaat ggtctaatgt aagctttaaa ttaaattctt ttgcaattct
     1621 tattgctgta aacatatcat ctgctctatg agcatgtgcc ttaaaaggaa tttctcttct
     1681 taaaactggt attaaacttt ccatttttat atcatattca ggtttttcac agtcttcatc
     1741 atcatgactc tcatacatat caatatcctc taaatattct tctgcttttt ttaggttttc
     1801 tctaagtaaa gcagctatag ccattctagt ttgaggagat ttatcatcct gaccataaca
     1861 gctctttgga ttttctccaa aagcaatttt agatgcaact ggatttttta ttaccatttt
     1921 atcaattctt tttccacatg tctttatagc tatacattga ccacccataa catttgcact
     1981 tccaggggtt gtacaaacag aagttattcc accttcaaaa gcttctttaa aagttctatc
     2041 cattggatta attccatcta taggatttaa ttgtggtgtt attgggtcag tctcttcatt
     2101 tccatctgct ccttcaaacc ctataccatc ttcccataag cctaagtgag tatgagcatc
     2161 tataaatcca ggaaacacaa gtttattact agcatctatt acctctacat ctaaaggagc
     2221 tatcaaatct tctccaattt ctattatttt tccttcatct ataagtatat ctccatgaat
     2281 tatcccattt gttatggtat ttattttacc attttttata aaaatcattc attttcctcc
     2341 tgattttcaa gactatcgtt cttattttaa tatattttct tactatatac ttttacaaat
     2401 ttttaacatt tatacactcg agtaatctta tttacaaata tagaatctaa gtactataac
     2461 catacataaa cttatctatt ttttcattga taaggcaact ctttttttgt ttaagtcaat
     2521 tccaatgact tttacatcta ctatatcacc tactgtaaca atactcatag ggtctttcac
     2581 aaaactatta ctcatttctg atttatgaac taatccgtca ttttttattc caatatctac
     2641 aaatgcacca aagtctacaa catttcttat tgtacctttt aaagtcatac cctcttgtat
     2701 atcttcaatt tttaagacat cagttctaag tataggtttt ataccttctt ctcttgggtc
     2761 tcttcctggt tttttgattt cagctatgat atcttttaat gtaacttgtc ctacttccag
     2821 tttttcactt aattccctca agccaatttc ttttattttt tcatctattt cacctatatt
     2881 cttattcttt acatcatcta aagaatatcc aattatctca atcatttttt tacatatatc
     2941 ataactttca ggatgcacac ctgtattatc tagtggattt ttaccctcta agattctcat
     3001 aaatccagca cattgagtaa atgcttgagg tcctagtctt tttacttttt tcaattgtgc
     3061 tcttgacgta aaatctccat tttcttctct atatgctatt atattttttg ctatagcttt
     3121 acttattcct gcaacatgtt ctagtaggga gtatgatgca gtatttaaat ctactccaac
     3181 actatttaca gaatcttcaa caactccatc tagtacttct tctaatctct ttttgtttaa
     3241 atcatgctga tattgaccaa caccaatact tttagggtct atttttacaa gctctgctaa
     3301 tgggtcttgt aatcttctag ctatagatat agctcctctt attgatacat ttatatctgg
     3361 atgttcctca tttgcaagtt cagatgctga ataaacagat gctcctgctt cactaactat
     3421 cacatactgc acttcgctat ctatttcttt tatcatttct gatacaaaag tttctgattc
     3481 tctagatgca gtaccatttc caattgaaat tatatctata tcgtattttt caatcaatcc
     3541 ttttaaaact ttctttgctc cctctacatc attttgaggg tctgttggat atactgttgt
     3601 ataatctagt agtttaccat ttttatctac tacagctatt ttacatccag ttctaaatgc
     3661 agggtcaaat cccataacta ctttgtcttt aactggtggt tgaagcaata aacttttaac
     3721 attctttcca aatacactta tagctctttc ttgagctatt tctgttaaat gatttcttat
     3781 ttctctttct attgatggga aaattaatct tttataagaa tcttctattg aacttacgat
     3841 ttcttcttta tttttgaaat ttttatcatt tacatattcg tttattatgt aatttaaaac
     3901 tttatcatta tttatttcca acttaacctt taggaagctt tctttttctc ctctgtttat
     3961 tgccaaaact ctatgtggag ccatactttt tactgcttcg ctataatcat aatacatatc
     4021 ataaaccgat ttctcatcag tagcactttt gctaactatc ataccttctc ttaaagctaa
     4081 ttctcttata tactttctaa tcttagcatc atctgagact agttctgcaa ttatatctct
     4141 agcacctttt agagcatctt caatagaaag aacttctttc tcttcatcaa gataattctt
     4201 tgcctctatt tctatattat ctaaattatt ctctagtatt gacaatgcta aattttctaa
     4261 tcctttttct tttgctattg ttgctctagt tcttttcttt tgtttatatg gagcatatat
     4321 atcttccact tcttgtaatg tttttgcttt ttctatattt tgtgttattt catctgttaa
     4381 tttcccttgt tcatctatta gtctaactac atcatctttt ctactttgaa gatttctcaa
     4441 atacattagt ttttcataaa attctcttag tgtaacatca ctcatttcgc cagtcatttc
     4501 ttttctatat ctagctataa atggtattgt atttccttca tcaataagtt ttaatgtatt
     4561 atttatttgt tcatctctaa gattaaactc tttttttaag atttgattta tatccaattt
     4621 attcactcct aatgttagtt tttcataagt ataacaaaac agaacgaagg ctgagggtta
     4681 tcgtcctcag cctaagtgct gtaagtgcat attaagcact aaccttctta tacatatatc
     4741 ttaattacag tgcatattta ttaaactatt ttattcattt ttaagtattc attaataaac
     4801 aagtctatat ttccattcat tacactatct acattaccaa actcagcatt tgttctatgg
     4861 tccttaacca atttatatgg ttgaaaaaca tatgacctaa tttgactacc ccaagttatt
     4921 tgtgagtatt tgccttgtat atcttctatt ttttcctttt gctctaactc ttttaattct
     4981 atcaatttcg ccattaaaag tctcatagct ctgtccttat ttagatgctg agacctttca
     5041 ttttggcact gaactactac accagtaggg atatgtgtta ttctaactgc tgaatcagtc
     5101 gtatttacat gttgccctcc agctccagaa gctctatatg tgtctatctt taaatctgat
     5161 ggattaattt ctacttctat attttcatct aactctggtg ttacatctat agatacaaat
     5221 gaagtatgtc tttttcctga tgggtcaaat ggagatattc tcactaatct atgaactcct
     5281 ttttcactct ttaaataccc ataagcattt gttccttcta caagaattgt agctgtcttt
     5341 atccctgctt cagggtctga tattatatct aaaagcttaa ctttataacc cttactttct
     5401 gaccatctta tatacattct caaaagcatt tgagcccaat cttgagcatc taagccacca
     5461 gtaccagcat ttatagataa tatagcatta tttttatcat actctccatt tagaagagtc
     5521 tttattttca tttcagataa tttttcttcc attgattcta tggacttttc tatatctctt
     5581 tctacagaat catcatcttc ttctaaacct atttcaatta atacttctat atcttctaat
     5641 aagcttttta agctttcata ttcttctaat gtctctttta aagatttatt ttcttgaagt
     5701 actctttgag cgacttcatt atcattccaa aagtcttgtt gattcatctt ttcttcatta
     5761 atttgtatat ttttcatcaa tgaagctatg tcaaagagaa accctcaatt cttctaaatt
     5821 tgaagccatc tgctccatac tatgtttata ctcttgtaac tttaacattt aatcctccga
     5881 gattatcttc cacaacagtt tttatatttt ttaccacttc cacaagggca taagtcattt
     5941 cttccaacct tttcatcttt tttaacagtt ttacttgttg gaagtgttcc atctgatgga
     6001 gaagatagat tttcaacatc tacaactgct tttctttcta ctggagtttc tattgttata
     6061 ttaaataaat atcttacagt atcttctttg atatgtttat tcatttcatc aaacatatca
     6121 aaaccttcca ttttataagc aataactggg tcttgctgac ctacagcacg aagaccaata
     6181 ccttggcgta attggtccat agcatctata tggtctatcc aatggttatc aacagcttga
     6241 agtaagatta ctctctcaac ttctctcatt ctctcatagc caacttgttc ttcttttgaa
     6301 gtatatatct tcatagctat ttcataaact ttttctgtta tttcaactgg attaagtttt
     6361 tctatttctg gtatttctat gctacctttt ggcaagaaca gattatacat atgttcttta
     6421 aatccttctt catcaaatcc tttatcttgt gtataaagag taactgcttc ttctattata
     6481 gaatgagtca ttgattgtat ttgttcttgt aaatcttcac cttctaatac acgttttctc
     6541 tctgcatata ttatttctct ttgtttattc ataacatcat catattgaag tacatgcttt
     6601 cttattccaa agtttttacc ttcgactttc ttttgtgctc cttcaataga cttagataat
     6661 attctatgtt ctatcggcat gtcttcttca agacctattt tatctacaat accagatatt
     6721 ctatcactac caaacaatct cataaggtca tcatcaagac ctatatagaa tcttgaacta
     6781 cctgggtcac cttgacgtcc agcacgacct ctcaactggt tatctattct tcgagattcg
     6841 tgtctttctg tacctattat agcaagtcca cctgcttcta caacttgttt ttgttcttct
     6901 tgtgtttgtt gcttaaactt ttcaaatagt ttttcatact cttctctagc ttcaaataga
     6961 atttcattac cttttacagg tataccttct attggagtat ctactctatt tactatttct
     7021 tctttaaatc catttcttct catttctctc ttagttaaaa agtctggatt tccacctaga
     7081 actatatctg taccacgacc agccatatta gtagctattg taacagctcc aagtctacca
     7141 gcttgtgcta ttatttctgc ctctttatca tgatgcttag catttaatac ctcgtgcttt
     7201 atgccctttt tctttagtat ttgtgataat aattctgatt tttctattga tactgtacct
     7261 acaagtattg gttgattgac tttgtgcctt tcaattattt cttgtgctac agcattaaat
     7321 ttacctattt cacttttgta tacacagtct ggtaagtctt cccttatcat aagcttatta
     7381 gtaggtactt ggaaaacatc cattttgtag attgctttaa actcttcttc ttctgttttt
     7441 gctgtaccag tcataccaga aagttttttg tacattctaa agtaattttg gaatgttaca
     7501 gtagctagtg ttttagattc tctttgtatt ttaagacctt ctttagcctc tattgcttga
     7561 tgtaaacctt ctgaatatct tctaccaaac ataagtctac ctgtaaactc atctactatt
     7621 actatttctc catctttagc aacataatca acatctttct tcattattac atgtgctctt
     7681 aaagcttgat ttatatgatg gtataattct gtatgagata tatctgttat attatcaaca
     7741 ttaaaataca cttctgcttt ttgtataccc gatgctgtta gagatacagc cttatctttt
     7801 tcttctaatt cataatcatc tggttttaag gttaaaacaa atgtgtttgc atctgaatat
     7861 aagtgagttg atttgtctcc aggtccagat atgataagtg gtgttctagc ctcatctatt
     7921 agtatagagt caacctcatc cactatagca taatttaatc ctctttgaac tctttgctct
     7981 ttgtgtatta ccatattgtc ctttagatag tcaaatccat attcattatt tgtaccataa
     8041 gttatatcac aatcatattg ttcttttcta acttttggat tttgaccatg tataataacg
     8101 cctacactca ttcctaaaaa ctcatagatt tttgccattt ggtctctatc acgttttgca
     8161 aggtaatcat ttactgttac aacatgtaca ccttttccag taagcgcatt caaataaaca
     8221 ggagcagttg caaccaaagt tttaccttca cctgttttca tttctgctat tcttccttga
     8281 tgtagtacta tacctccaat catttgaact ctgtagtgtc taagtcctaa cactctctta
     8341 gaaacttctc taactactgc aaaagcttct ggaagtatat catctatact ttcaccattt
     8401 gctaatcttt ctttaaatat atttgtcata tttttaagtt ccgagtctgc catagattca
     8461 aatttaggtt ctaatgaatc tattatatca acagttttat tgaacttttt taattctttt
     8521 ttatctgcca tgttgaataa attatccata aagctcatat tttacatctc tcctcgttct
     8581 ttattgtact attattcatt ttaccataaa aactatactt ttaatattaa tttaaaaagc
     8641 tatcgataat ttatttatcg atagctaata tgattaaatc taaattgctt tattcatgtt
     8701 ctattatacc atatccacca ttattacgct tataaactac tgctatttca tctgaatcta
     8761 tgttcttgaa catataaaaa tcatgtccta ttaattccat ctgtaataca gcttcttctt
     8821 cactcattgg cttaacacta aatttcttac gtctttgaat tactaattct tgattttcat
     8881 ccccatagtc ctcttgttca tcagctagat ttatctctgc atcttggaat cttatactct
     8941 tgttgtcttt atgtttatct tgtaatctct ttttatattt ttttaattgt ttacttaatt
     9001 tatcatatac aacatctata gctgtgtata aattttcctg catatcttct gctctaatta
     9061 taggaccatt tacagtagct attgttactt ctattttttg tcttgctttt ctcgcactta
     9121 ctgtaacttt tacatcagtg tctggatgaa tgtaattatc taacttactt agtttagttt
     9181 cgattttatt ttttattgca tcagttaatt cgatttgttt tccagatata attatgttca
     9241 taaatacatc tcccttcaat tatgccagta taagaaaatt tatactttaa taatatattc
     9301 tacacgaact ctcataatcc tctttttatt atttactttt atgatttttt ctattcaatt
     9361 gatagaatta tttatcattt ttagttacat tgatacttat gtggattaat attattacaa
     9421 acacctgtgg ataatgtgga taaaactgtt aataactctt gtttttgttt atttaaagct
     9481 atttttactc ctatttctat ccacaaaaat ctccattaat tgtttattaa tatgtgaatt
     9541 catgtcataa gatggtaatt ttaggaaatt atgtgtatta tatgccaata tacctttata
     9601 attttttaat aaatataaac cctctaaaac ctccataatc tatcttttct ttcattattt
     9661 atatttaaaa ctacttaaaa gttataaagc atactcaaca ataatttcat atttaatacc
     9721 tattaacatt catataccca aattataatt aacttactct ctttatttaa actaagtatt
     9781 ttatattcat atactatatt gaaattatat ttacgagttg tttaagcttt tgtctatcta
     9841 tatcttcaaa aggactgtta tttattctaa tcgctgtacc aaaatgaaaa ttacttgcct
     9901 tagttaattc ctttatcttt tctatattat taaaattaag acctccacct aaaagaattt
     9961 ttatgtgatt agaactaagc atcatattct ttatcatttg tatgttatca actatacttc
    10021 ctttccctcc tgatgttaat atattcgtaa ttttatcata atccttcaaa attttaacac
    10081 tatctattgt atttgattca tctatggctc tatgaaatgt aacatcaaga ttatcacaac
    10141 atttcaataa aacatttaga tttttctcat ctatattatt gtttttatcc aatacaccaa
    10201 atacaactcc attagcacca atctctttaa ccacacctat atctttttgc attatactta
    10261 tatcttcttt actgtataca aaacttttcg catgatgtct tatcattatg ttcacaggta
    10321 tctttacact gttaacaaca ctttctataa gaccaaaact tggtgtcaat ccaccttcag
    10381 ttaaagcact aactaactct attctatctg caccacaatc ttctataatt ttagcgtctt
    10441 ctactgacat tcctattatt tctaacataa ctactccttt gctaattcat atattgcatt
    10501 tgcaaatatc tttgcattta atattatatc ttctatatcc atgtattcat caggattatg
    10561 agctattcct ttttgtcctg gaaatgaggg tccaaacgga actatatttg gcattacttt
    10621 tgcatacgta cctccattag ttgttactgg agttccatct aatgatgtaa ccctttcata
    10681 agctagctga agctttttaa caagattact ctctttatca aaatatacag gattaaaatt
    10741 agaaattacc tctatatcga tactatttgg aaaactagct tttatttcat ctaatacatc
    10801 tttcaattta taactaaaat cgtattttac ctttatatct aatatagtat cactattttc
    10861 tatattcagc tctttatatg taacaagcac attttcagac atattttcta taaaacctag
    10921 gtatacatct tcttcataat tgattttact ttttatttta atctttgcca tgcctttttc
    10981 accatataca acaggatatt tacaatctgg tgtaaatccc ataataggtg ctttttcttt
    11041 ttctaaataa tatggtatat cttcaaatcc actttcttca tttgtaccaa atattattct
    11101 aactctctta tctaatttta agtttaattc tttaatagca tataatccat ataaggcaga
    11161 cattataggt cctttattgt ctagtactcc tcgtccataa attcttccat tagtttcttc
    11221 tcccttataa ggctgatgct tccaaccatc tccttcatgt acaacatcta catgtccaat
    11281 tacacaaaca taatcatcac tatctccatg ctctgcataa ccaatataat tatccaagtt
    11341 tctaacctta aatcccaatt tttctgatat ttctaatgct ttattaagtg cctcccctac
    11401 tttttcgcca aaagggaagc cattttctga ctcccctttg acactaggta tttttacaat
    11461 atctataata tcacttaata aatcttcttt taactcatct attttattac ttatgatatt
    11521 cataaaaatc tccttattat aaaactctat tttaattatg taaccactac tttactcact
    11581 tacttctatt ggtgctttta ttctagcctc atatgcttcc atccactcat ttgatataac
    11641 cttatgtata gtctttccat ctactctatt tgaaatatat acagtaggag attcttcttt
    11701 agttactact gaaaaagtaa aatcagctat atttgtagct actccccatt cacctttatt
    11761 attcatggca actaatgata aatcaccagc ttttcccctt ctttcgataa gctgtttatc
    11821 aagttcattt actgcaatat cacatgcttc ttgtggatgc ttcccttctt tcataagtct
    11881 aactatctca taagatatac atcccttcat taaatcttct cccaaaccag tagctgtagc
    11941 tcctcctata ctactatcaa catagaatcc tgaaccagat agtgcagaat ctcctattct
    12001 acctcttttc ttcataaaca atccacttgt tgatgtagct gcacacattt ttccagtttt
    12061 gtctaaagat atcataccaa cagtatcatg accagcatat gggcttaatc ctttgtctaa
    12121 agtctctttt tttctctttt tataatgtaa cttcgctcta tcagtcaaca tattttttct
    12181 ttcaaatcca tttttatgag cataatcttc agcacctatt ccaactaaaa aattatttac
    12241 cttctcataa cttaacttcc tagctatgct gacaggatta gaataatctc ttataccagc
    12301 aactgctcca atatctaatg tgttaccatc cataaatgca gcgtcaagtt ctacctcaca
    12361 attttcattt ggaagtccac catatccaac cgatttatag aatggatagt cttcaactct
    12421 tcttacagca agctctacag catcacctgc ttccatgcct ttttctaaat gttctgatgc
    12481 ctcagttatt ccttctaggg ccattctcca tgtcgctatc attccccaca tgttaaaatt
    12541 cccctcctta aattatatag attttactaa tgtatgctta ttataaatag tataatattt
    12601 aaacttgcta ataattaata ctcttattaa taacaaaatt cttctttcat caaagttata
    12661 tcttctttat ttgctattgc ttttgccaat ttacatatgt tatttaatga atcattaaga
    12721 ccaattccac cttttttggg agtcttaact atacatatgt catttttata ttcgtttatc
    12781 acatctatat tttcttctga catagctgca aaagctggta tatctgtttt atcatttata
    12841 tttttagaca gtattgctga catcttagaa aagttttcgt aattaaagct tggtccacag
    12901 attactacat cagggtttaa tttttttacc atagctatca tcttactcgt tacttcttct
    12961 tgattattta caaaaaattc atccccacaa aataaggtag ctattacctt agattcattt
    13021 aaaaattgct ccatcattac acctggtcct aatgggcttg attttccagc aggaggtata
    13081 ttacttttct cttttcctcc agctccagct tgtatttggt ccaatatcat aactattttc
    13141 ttcataatct tcctcctata atactaaatc atcaaaagat atatcatcat cttcctcttc
    13201 aacagtttca ttttcttctc tcaagtattg cttatctagc atttttataa atggtatata
    13261 cataaatatt cctagtataa gaagtactaa ttgtaagact gcaccttgcc atcctgttgt
    13321 taaaaaacca gagaatataa ttggagttgt ccacggtaac tgaactccat ttgtaagagg
    13381 cactaatcct gctgacatac agaagtaagc tattattata tttattgttg gaactattgc
    13441 aaatggtatc aatagtattg ggttaagcat tattggtaga ccaaatatca atggctcatt
    13501 tataccaaat attcctggta aaattgatag ttttcccatt gactttatac gtttactttt
    13561 acatattaat aacatagcta tgactaatga tagtgtacta cctgctccac caaatgtcgc
    13621 aaacatatct tggaattgac ctgttattat gtttggtatt tttgaaccag cttgaaaagc
    13681 agctaagttt tcagctgata gaatttttaa tattggatta tacacagccc caacaacact
    13741 acttccattt attccaaaga accagaataa atgtaagaat atataagcga ttgccatagc
    13801 acctaaacta tttcctaatt ttaataatgg catttgtaaa aacttaaata taaattcatg
    13861 tatacttcca tatggtgtag cttcaaaagc tatcttaact aaaaagaaaa ctaccataac
    13921 tattgcactt ggtattaatg ctgcaaatga ttttgataca gttggaggaa ctccatctgg
    13981 cattttcaat gtccatcctt tttttacaac tgcagcaaat aattttacag aggttattgc
    14041 tgttatcata cctacaaaca ttcccttaga acccatccaa cctagtggta tacctgttac
    14101 catgtaaact gattctgttc cttctggtgt atatggtatt acaaatggtg ttattataaa
    14161 aaaggcaact attgcaactg ctgctgatgc tattgggtct acatctattt gctttgcata
    14221 agaatatcca atccctaaaa ctgcaagtag agtcattact tcaaatgttg ctacagttgg
    14281 ttgttgcaat tttacagccc aatctggacc aaaaatttga cctacaaatt cattccatcc
    14341 ttttatagga aaatttgcta ctaataaaaa taatgaacct attattaata atggtgatga
    14401 tactaaaaaa ccatctctaa ttgcaattaa taatttattt ttacctattt tttcagccaa
    14461 tggcataagt attctttcca atctattcat ctatccaccc tcatttccta ttatttttta
    14521 ctataattct acgcttgttt agattttatt agctttatag caagttttaa tactttttcc
    14581 ccattcatag ttccataatc aacttcattg attatatcta taagttttcc ttgagcttca
    14641 aactttggtt ttaactcctt tagcatgtat ttaacttgtg gtcctaataa tatacaatct
    14701 ggattttttt cttcaactat tttgtcaatt tctgctatcg gaaatgcctc tacttctatt
    14761 ggtaaattgt gttcatctgc taccttctgc attttatctg caagtaaact tgttgacata
    14821 ccaaagctac aaaataaata tacttttctt ttcatttttt aaatccccct taattttttt
    14881 attttaattt ttgaatttct tttcttaaca taatcatttc ttttataagg tttctctcac
    14941 tcatacaagt cattaaatgg tcttgagagt gaatcaataa aatagatata tctgatttat
    15001 ctcccccagc ttccttttgt ataagttcag tttgtacttt atgtgctttt aaaatttctt
    15061 catttgcatt ttcaattaat tcatctgctt ttttaaaatc gttttccttt gcatagtcaa
    15121 gtgcttcata aagcatagat tttgcatttc cagcatgaat tataatcttt aaaactattt
    15181 cttccatttt ttcctcctaa gttgcttctc tacttttaga ttatttatac cttacaaaca
    15241 tataatatct tataaaaaca aaaaaacatg cacaactttt tccatcacat ggaaaatttt
    15301 gtgcttatcc atagttcact atgaatttta tataagaata tcttttacct tttcaatgtc
    15361 acatttactt agaatatcta gcatattttc atcctctgtt aaattagata tttctttaaa
    15421 taattctcta aattcttttc tatcatcatc ttttttatat gctaacaaaa gtataatctt
    15481 tatatccttt ccatttctaa tttttattgg tgtttttaac cttgcaaaac ataaaacaga
    15541 ttgttttata tatttcatgt ctccatgagg gaataatatt ccttttccaa cattggttgg
    15601 atatgacttt tctcttgtta taatagattg taaatactct ttttctgcat aatgtttttc
    15661 aattaataaa ttagttaaat atgttatagc ttcataaata tcatcatgtt ctaactctct
    15721 tattaaataa tcagacatca ttaatctgtt cataagttta tatgaagtgt ctttttcttt
    15781 tattttgttt gtgaactctt taagtttatt ctcatcctca tcagtaaata atggtgttgt
    15841 tataaaaatt ggtttttgga tattttcata ctcttgaaat gcaattataa aatctatagt
    15901 cttattaaag ttatctcttt ttaaatcttc ctctcttaat gtttcaataa tttttatgtt
    15961 atcaaatctc ttttctatct ttacttttaa tagcatactt actccaaaac taaatggaca
    16021 tacaatactt gcctttttat ttgattccct tttgctgtat tttctttcta gagaggtttg
    16081 aaagtgaagt gtaagataac caatttcatc ttccgttatt tgttttcctt ttaattcaat
    16141 ttctattgaa cttgatatta ctgtaaataa aaaacaaaat ttagttttta tgttatctaa
    16201 aagtggattt tctaagtaaa cattgctttt catttgatgc attgtaacat tcaaatgaca
    16261 tattaattgt tcataaagta atatatcatt attaaaattt attcctgatt cttctgatac
    16321 taaatttata attttttttg tataacttgc taattcttca tttagatata aagatttctt
    16381 aatattatta ctatttattt gtttgttcat acccaatatt aaagattcta taaacaattt
    16441 ttcttcataa tttaatacta tggataaatt agatttcaat tcatcttcta tatccaatat
    16501 aagcattttt agcttaagct ctttcttagt atttgatact tttaaagaat ttcccagcct
    16561 tattcgtatt atatttataa gcaaaaatag catcaattgc ttgaatgata tatctgtaag
    16621 tcttatatcc atatcctcct ctatagtaag cattatgttt tttacaatta ttatatctac
    16681 tatatggaag tactctattt catctatgtt taatctgtat ttatctatat atttaaataa
    16741 gaatgatact aacattattc ttatgtcttt ctcatcccct tcaatatata taccactgcc
    16801 ttttttagtc gagatattta aattataatt atttaatgcc actgataatt tacttatttc
    16861 ttctctgaca atatttttat ttatgtacaa cttattagat aaatcattta aagtaacttt
    16921 tttattatta caaagtagta aatttaatat ctcaacttgc ttatgaagct cactatttac
    16981 tcttttaact tcacctcgca acttctgatt taccagtatt ttgtctgttc catctccact
    17041 aaatttaatc ccaatattag gttttctttt gatataagca ttacaaaaag tagataacca
    17101 ttcatcaata tatttgcaat catttcttat tgtcctttct gaacactcaa aatcattggc
    17161 aatcttatta acatttataa actcttcttt taatagcaat gttgttaata aattttcttg
    17221 tcttttattc aaacatatca cctctttatg actcaaccta aatttattat atataaaaaa
    17281 gagctataca taaaggtatg ctagctctct tttaatatga tatttagctg acctggtttt
    17341 tgaccccaca ctcgcctact ggagggttcg cttgggttcg ccgcactact actctctctc
    17401 gattttatta tcggtaggtc ttacttctaa gccgcaccat gatcattaac ccaattttta
    17461 gtgttaactt acgtggatcc ctttgcctgc gactggcttg gactttcaac cacagaccta
    17521 aatatcactt attcatttta atattttaaa ctaaaattgt caatatataa gattattcca
    17581 taatataatt atcatttgct tttgtaagta gagtcaatac aaaaacctta ttagcacctg
    17641 ataactttaa aagtttagaa atttcgtttg ttgttagacc agtagtaaat atatcatcta
    17701 ttaaaagtat atttttatta tttattaatt taacattttc tttaacaaca aatacatttt
    17761 ttaattcttc ttttcgttct ttcttattca atttatatag cattcttgta tatttttttc
    17821 tacttatgca atctaataat ggaatattta ctattttact taaattaaat gctatttttt
    17881 gtgcctgatt aaaacctctt ttatttagcc tttttttatg aagtggaaca aataatatat
    17941 aatcaaattt tatgttttct aaatataatt tttctttcat tatttgagct atatacttag
    18001 ccataaaggt tttttggtta tacttcagcc ctaacaccat cttttttgtc acatcattgt
    18061 attcaataca agatattgat ttatcaaaat aaaagtcttt attaaaacaa taactacact
    18121 cttcaataaa ttctttttca attgaatgac gtattattgg ttttccacac ttcatgcaac
    18181 catcttgtat aaaattcatt tctttaaaac aacttttaca tattgaataa gtatttgttt
    18241 tctttatgga tttatcacaa attatgcaac ttatattctc aggatatata aaatccaaac
    18301 atttattaat catattttta aaattcttaa taaaattatc attgcatatt ttcatattta
    18361 ttctatcaac aacccttctt gtttaaattt atttagctta tatcctaaat ttgaatacct
    18421 ttgattaact ctattatttt ttatcatata ttcaagatat ttcacatctc caactaatac
    18481 aactaatttt tttgctcttg ttactgctgt atataataaa ttacggctaa gtaacatagg
    18541 aggagcccac gctattggca gtactacaac tggaaattca cttccctggc ttttatgtat
    18601 tgttgtacaa aaactatggt caatttcatc taattcatca tataggtatg atacaatttt
    18661 agtctggtca aataatatat aaatagtttt cttatcttta tctatgtgat aaacatatcc
    18721 tatatctcca ttatagatac cttctccact ttctttttgg tcttctgttt cccatttttt
    18781 tgtataatta ttttttactt gcattacctt atcgccaact ctaaataatc ttttagaaaa
    18841 actttcttca actttaaatt tttctttttt gttaagatac ttctgtaact ctatattgag
    18901 attagtaact cccaattcac cttttctcat tgaagataaa acttgtatat cttttaattt
    18961 atcaacttta taaaatttag gtaatctttc attaaccaat cctattattt catttaaaat
    19021 ttcttcatta gttgattttc ttataaaaaa gaaatcttta ccttttgtat ttagataaag
    19081 tggttctcca ttgtttatct tatgggcatt tacaattatc atactttctt gcgcttgtct
    19141 aaatatttca tttaatttaa ctacatttat aatattagaa ttaatcatat ccttaagtac
    19201 atttccagca cctacagaag gtaattggtc actatctcca actaaaatta ctcttgttcc
    19261 taactttata gctctaagta aattgtacat taagataatg tctaccattg atacctcatc
    19321 aacaattatt acatctgaat ttattgggtc ttcttcatcc ttaaaaaaag ttaaatcatc
    19381 atctgttgaa aatcccattt ctagcaacct atgtatagtt tttgcttctt tattagaagt
    19441 ttcactcatt ctctttgctg ctcttcccgt tggagctgcc aaagtcacac ttttaccatt
    19501 attttcaaat atatctatga tagcattaat ggtagttgtt ttaccagttc ctggtccacc
    19561 agttataatt aatactccac tattaacaga ttctttgact gctaaaattt gtttttctgc
    19621 taacttaatt ttcttatctt cttctaaaac attaatctcg ttatctatat ctatttttaa
    19681 gtcttcaaat tcatactgtg ataattttac tatttgacta catactccat tttcagctaa
    19741 atagtatggc ataaggtata taagattttc attgtttact ttctctatat gtattttttg
    19801 attataaact agcatcatta cacactcttt tattatttct ccatttagct ccaataattt
    19861 ttccgaatct tgtattagaa catgctctgg taaatatgta tgcccattac ttaaagattt
    19921 atttaaagtg tataagattc cctgacaaat tctatcttta gagtttttat ctataccaat
    19981 tttacttgct atactatcag ctactttaaa tccaattcct cttatatctt cagcaagttg
    20041 gtatgggttt ttatttataa cttctattgc actgctttta tattttttat atatttttag
    20101 gcagtaattt ggtgttattc caaaaggaga tagctgtatt attatatttc tcaattctct
    20161 gtcttcttca taacttttta ctatttgttt tactttttta ctccctatgc cttctacttc
    20221 ttgtagtttt tctggtgaat tttgtatcac ctctaacgta tctacaccaa acttatcaat
    20281 tattcttttt gccatttttt caccaatacc atgtatcatt ccagaagata agtaaacata
    20341 aattccttct aaggatgatg gtgttactgg cataaaacta ttcacttcaa attgagtccc
    20401 ataaatctta tgatttaccc actttccttc aacttctatg ctttctccaa ttgccaaagt
    20461 aggcatacat cctacaacca caatttcatc attttcattt accaggtgag ctattgtata
    20521 accattatct tcgtttttaa agactatttc acttatcatt ccttctaatt tttccatttt
    20581 tatctcctaa aaatactaat aatcgctcca tttaaatgtt agtataattt tatatattaa
    20641 aactatgata atcgtagaaa agttttatat ttttatactt aacgtaattt ccaactatta
    20701 tacattttat cagaaacaaa aaaaatctgc atccataaaa tatggtacca tgaaatacag
    20761 gtaccatatt taaatagcta ttttatttta attactctat atttagagta taaattaaat
    20821 ttcataagta tattacagta ctaacttatt tcagatttat tctccttgta cccctcagtt
    20881 ttagtaattc ttctataaag catacatagt aaaattaaaa ttcccatgta acctgtatat
    20941 ggatatagtg tacctactaa cttatcaaat ggcagtaaac ctcctataca agctaatata
    21001 cttaccacta ttgttataat tttaaactta gggtggtcat cttctacaaa tcggtttgtt
    21061 actgaccata acaatggtac agctgtagtg tatattccta gtaaaagtac tactgagaat
    21121 aaaataccta ttattggaga tatcttatca gctaagtaaa gtgctggtat ctcttttgtg
    21181 tatatatttc ctatgtctga aagtaatgcc aaattcatca ttatagctgc tgccataagt
    21241 gctactccac ctactatacc accccataca gcatctttct tatttactgc agaagctccc
    21301 attcctgtca agaatgttat tactattatt acattgtaac aagtataaag tactcccgag
    21361 aaatatccat ttgatgtagc agttttaata tttaaactat caaccatatt tccagcattt
    21421 gataaagcat caatattgct aaataaacta attgctccta ctaaaagtgt aaatattatt
    21481 attataggtc ctatattacc tagtatttta gaaagccttg tcaaacctaa tgaaacagtt
    21541 attagggaaa cgattgccat accaatccta cctacatatg gatttaatcc atagtactct
    21601 gataatgtag ctcctgctcc tgcaatcatt attactaatg ttccaaataa aaacaatgga
    21661 ccaaaccatt caaaaaatgt tccaagatat tttccacaat atacttgata aatttttatt
    21721 ggctccttaa gtttcaattc tcttccctta tctataactt ctgctccaaa ccatgaaaat
    21781 agtaccatag agattattcc acctataata cccgcatatc cataaaacgt aaaaaattgc
    21841 agtatttctt gaccagttgc aaatccagaa cctattacag tagcaacata agctccagca
    21901 aaacttataa ccgttttaat atttactttg cctgacatac actactcccc cccaattaaa
    21961 attattttta aagtgtaagt ttattaagat tatattagac tcattattgt gatatttcaa
    22021 tatattttta attatttaac attagttaac acattcttaa ccatccttta acatatatcc
    22081 cataatatgt ataaatttag tagagtatta tataaaatgt attgaccaga ttaaatactg
    22141 tttattcatt atatgatgat aaattaacat ttattaaaaa tatttaactc ataataaaaa
    22201 agctgttaga aagtaatcgt aaaattacat tctaacagct ttttagttaa aaattattca
    22261 cctaaagcat cttttcttaa ctgctcaacc ttgtcagttc tttcccaagg taaatctata
    22321 tcagttctac caaagtgacc ataagcagca acctttttat aaagaggctt tcttaaatct
    22381 aagtctctta ttatcgctcc cggtcttaaa tcaaagtgtt tatttactaa ttcaacaagt
    22441 ttttcttctg aaaccttgcc tgtcccaaaa gtatctataa atatagataa tggtctagct
    22501 atacctatag catatgcaag ttctatctca catttgtctg caagaccagc cgcaacaata
    22561 ttttttgcaa catatctagc tgcataagca gcagatctat caacttttgt agggtctttt
    22621 cctgagaaag ctcctccacc atgtctagaa tatccaccat aagtatctat tattattttt
    22681 cttcctgtaa gacctgtatc tccttgtggt cctcctataa cgaatcttcc tgttggattt
    22741 atataaattt tagtctcttc atcaagtaat tctgctggta taacttcctt aataacatga
    22801 ttaattaaat cttctcttat tttatcattt gatactgttt cacaatgttg agctgatata
    22861 agaactgtat gtactcttac agctttactt ccttcatatt caacagtaac ttgagttttt
    22921 ccatctggtc ttaaataatc aactaaacct gtttttctaa cttctgtaag tcttcttgat
    22981 aatttatgtg ccaaagatat cggaagtggc attaattctg gagtctcatt acatgcaaat
    23041 ccaaacatta taccttggtc tccagctcca actttttcta tttcttcttc tatctcttct
    23101 ccagtcttac tttctaggcc ttcatcaact cccatagcta tatctccaga ttgttcatca
    23161 atagaagtta taactgcgca agtatcacaa tcaaatccat attttgctct tgtatatcca
    23221 atttctctta ctgtttctct tactaattta ggaatgtcta cataagcaga tgtacttatt
    23281 tctcctgcta ctaaaactaa accagtagta actgtagttt cacaagctac tctagattgt
    23341 gggtcttttt ctaataatgc atctaatatt gaatctgata tttggtcaca tatcttatca
    23401 ggatgtcctt ctgtaactga ttctgacgta aataaatgtc ttgccatttt tattccccct
    23461 tatttattct taatttttat tataggtttt accttatttt taattttaga aatttactca
    23521 aaataaaaaa acctctcctt tataagaaga ggttaataat cgtaattaca tactaattaa
    23581 ctgacctcat ctttcaagac ttttgtcttg taggaattag caccttttcc catttgggtg
    23641 gttgctgggc ttcacagggc ctatccctca gcctctcttg ataaggtaat caagatttaa
    23701 gttttgtttt actttactag cttactattt ttttaaaatt ttgtcaatat ttttagtcat
    23761 attttttcta ttcctttttt tgtaagacaa ataaaatttt ctatctatct gaaatttaaa
    23821 gttaggtctt tttaatgctt tttattcata tatttttttg aagggggtgt tcctattgta
    23881 tttagctaaa gtaaactaca gcgatgatga tgttatacag atgctaagtt cagttctaaa
    23941 aactataatt aatgaaaata cgattgttgt ttgcatagga acagacaggg ctataggtga
    24001 tactttaggc cctttagttg gaacaatact taagaacagt aactttaaat accccgttta
    24061 cggtacacta gataacccta ttcacgcctt aaatatatat gagtctttag atactataaa
    24121 aaatacacat atacaaggta acttcttggc tatagatgct tgcttggggt cgcagagtaa
    24181 cataggtaat atacagatta gagaaggacc tatcctgccc ggaaaaggtg ttggtaaaaa
    24241 actacctcaa ataggaaatt attctatagt aggaattgta gataaaattg atgaaaataa
    24301 taaactttca tttaataata tacgccttag ctttatatta gatttagctg aaacaatagc
    24361 cttggcttta ttagtatcca cctaatctaa atataaacta aatgataaaa ttctttatat
    24421 aaaaaggtag atattttaga gtatctgatt aaatactcca aattatctac ctttttactt
    24481 ttatatttca agtttttatt tttctactaa agcaaacatt aattcacctt tacagacaag
    24541 ttcttcacct acatatgcct tagcatctgc cttagctata ttttttctaa gagttgtcat
    24601 ttcaacttcc atagttaatg tatctcctgg tcttacttct ctcttgattc taactttatc
    24661 tatacctgca aatacaccta gttttccttt gttttcctct acagacatca ttgccactcc
    24721 acatacttgt gctagagctt ctactattag tactcctggc atcaaaggat attctggaaa
    24781 gtgaccttgg aaaaatggtt cattaactgt tacattcttt ataccaacag ctcttttccc
    24841 aacttctagc tctgtaatct tgtctactaa taaaaatgga tatctatgtg gtattaattt
    24901 ttttatttca tctatattta acattctaaa ctctcctaat tattttcttt tgaaagaccc
    24961 accagtttca agtaagtcta ttgaacctaa agctattcct gtaccttttg caacacaaga
    25021 taatggttca tctgctattg taacagttat tcctgtcctc tgttctatta ttttatctaa
    25081 tccatataat aatgaacctc ctccagtcat tattatacca acatctccta tatctgctgc
    25141 taactcagga ggcgtttttt ccaaaacagc atgagtagca actactattt gctcaacaca
    25201 ttctctaagt gcttccaaca tttctgttga atttatagtt atagaactag gaagtcctgt
    25261 aattaaattt cttcctgtta tcttcatata tttttcttct tctctcttaa atgcagaacc
    25321 tatttcaaac ttgattttct ctgctgttct ttctcctata agaagattgt gttgtttttt
    25381 catatattta actatagcat catcaaatct atctccacca acctttatag attcacttac
    25441 aacagctcct cctagagata ttacagctat atctacagta cctccaccaa tatctataat
    25501 catattacca ttaggctgtg atatgtctac accagctcct atggcagctg ctattggttc
    25561 ttctatgata tatacttctc tagctccagc ttgtctcgta gcatcttcta ctgctctttt
    25621 ttccacttct gtgacacctg ttggtacaca taccataatt cttggcataa aaaatcttcc
    25681 aaatcccttt ttatcaacta tcttatctat aaaagatttt aacatcttct cagttacatc
    25741 ataatctgaa attacaccat ctcttaaagg tcttatagcg actatattac ctggtgttct
    25801 ccctatcatc tttttagctt cttctcccac agctaaaaca gaatctgttc ttttgtctat
    25861 cgccacaaca gatggttctt ctaaaacgat tcccttacca ctaacataaa caagcacatt
    25921 tgcagtacct aaatctatgc ctatatcagc tccagccatt ttttcaactc ctttatttat
    25981 aatattagat tatattataa gcctatttta atactcctat aataaagcat atacaaaaat
    26041 ctcgctttaa tcaagagaaa ataaatcttt tgagagtatt attattttgc tatttataat
    26101 tacttaatat atttcttttt ataatttaat tcaataaaaa aagtaggttt ttaacctaca
    26161 tttttttatg ttccttctca tattttaatt tcgttgctaa tccccctcta atatgtctct
    26221 ctgatttatt tttgtccagc acatttttaa cttctttggc aagggatgga tttatttcct
    26281 tcaatctttc tgtaacatcc ttatgaattg tacttttact tactccaaat gtcttggcgg
    26341 tttgtcttac tgtagtgttt ttttcaagta tatattttgc taccactatg gccctttcct
    26401 ctatatgaga tctcaaagat tacccccctc acataatagc tttaatgata tatatgtaaa
    26461 aacaccaaaa aatataacaa gcttaaccaa atatgattaa acttgttata ctattactta
    26521 attagactca ttgggtctat agatttttct ccactatacg cctctaaatg cacatgtata
    26581 ccttcttcac tttctatttg agaagtacta cctacagtac ctatagattg tccttctgtt
    26641 accttctgtt ctttcttaac actaacattt ttatctagat ttgaatatac tactacaatg
    26701 ttattgtcac tttttatttt gactgattgt ccatattctt catcatcaaa tacatctact
    26761 actattccgt ttaatagaga ttttacttct ttgcctttag tacaactaat atctacacct
    26821 ttatgagttt cccaaacatc aagtgtttta gaataactag gctctttttc tgaatatcct
    26881 cttattactt tatctccaat gtaatttaat tttgttgtac tcgatttatt ttcctctttt
    26941 gacttagctt tttctaagtt ttgttttgaa tccgttgcag tagggatagc atctttcttg
    27001 tctttttcaa ttaaatgtat ttcctcttca ctatctttat tagcattttc cataattcct
    27061 ttattagaag ccaatttatc tacattatta tttgtaaacc aaactccacc tactgctaat
    27121 aaacaaacac atacaaacaa agataaataa aaaccatctt tttctaacag ctttttcttc
    27181 attttgcacc tccaaatata tttctacatt tggattattg acgtgttttt ggcattttat
    27241 acaattatta gtatatatct tttatttttg tatctgtgta gtaatgtgat aatatatcat
    27301 aatatttata gccctcttct gccattcctt ctgctcccca ctggctcata ccaacaccat
    27361 gaccatatcc ttttaccacg aaatctatgt atccttctcc aaattttatg tcaaagtttg
    27421 cagagttcaa tttaaaaaca gttctaatgt cttttccagt caattcttta tttcctaatt
    27481 ttattttttc tacagttccg gcatcacttc ttttagttat tgaaacttgt ttacttaaat
    27541 tattcacatc tatcacaata gtgctatatg cccttctaag acttttaaca aaatctgtgt
    27601 tacttatttt aagtgttgac gcaaatttag gggaatactt atcataagga ctttccacag
    27661 actttaaata aggatatttt gcagaaaata cttcttcact attttctgtt tttcctgaag
    27721 atgtagaaaa ataaagagga agtatcgcct tatcattata agttattatt tgacctttag
    27781 ttcctctaac tgcttcttgg attttggaat atttattctt aatccattct tcaccattta
    27841 attttttaag agtatcataa ctcttatatt cttgacaatg tttataatca gtacatacta
    27901 ctgcattttt atgtttacta gatttaccat gttcctgttt atatactaca tatgttctag
    27961 ctgctacaga ttgagctttt aaagcttcta tatcaaactc tgaagacatc tcaccagcta
    28021 gtactccaca taaatagttc tccatgtcca tcttttcggt tttccctgtt atatgattat
    28081 atacattgat aattggtgct tttttattta cagtttcata gttcactgta tttttttctt
    28141 ctttattacc tttgtcttca atatcacttt ttttaatagt tttgtttata gatactgggc
    28201 tctctggttt ttcagttaat tctacatttt tataagaaac taaagttata agtgatggaa
    28261 ctaacacaga gcaagtcaca aatcctaaca aaacaaccaa tgggttcttc atatactctc
    28321 ctccttagct tttaaaacct tgtatttaag tatatgaaca accctattaa tttataactt
    28381 tcttctataa accaattatt aatcttctac tatttcgatt tgaccaccta gtgctgttat
    28441 cttcttatct atatcaacat aacctctttg gatatggtat atttctccta tttgagtttc
    28501 tccttctgct ataagaccgc ataaaataag ggctgcccct gctcttaaat cagtagcgtt
    28561 tactgctgca ccgtgtagct catctacacc attaacaacc gcacttcttc catctatttt
    28621 tatattagca cccattctat taaattcagc aacatgcata aatctgtttt caaaaacagt
    28681 ttctataact acaccagttc cattggctac agtaagcata gccataaatt gtgcttgaac
    28741 atctgtagga aatcctggat gtggtaaagt ttttatatct attggtttta atacttttgg
    28801 acctacaact cttacagaat tgtccatttc tgttatttca caaccagctt ctcttaattt
    28861 agctacaact ggttttaaat gctccattaa aacattttca acagttatat caccttttgt
    28921 catagctgct gcaaccatat aagttgctgc ttctattctg tctggaatta cattatgttc
    28981 tgctcctttt aactctttaa caccttttat ttttatagta ttagtacctg cacctttaac
    29041 atctgctccc atttcgttca agaagtttgc taaatctact atttctggct cttctgctgc
    29101 attctctatt attgtagtcc cttctgctaa agaagctgcc atcattatgt tttcagttgc
    29161 tccaacagat gggaaatcta aatataattt atttcctact aacttttctg ttgctgcttc
    29221 aacaaaacca tggtccattt ctatctttgc acctaaagct ttaaatcctt ttaaatgtaa
    29281 atctataggt cttgttccta tagcacatcc tcctggcata gaaattttcg tactattaaa
    29341 tctagcaagc aatggtccca taaccaaaaa agaagctctc atctttctta caagctcata
    29401 tggagcttca caagttttta tattgcttgc atcaacagta agtgtatttt ctttatattc
    29461 aacttctgct cctacatgtc ttaataaatc tgatataaca tgaacatctc ttaaatttgg
    29521 tactccattt aatgttgatt ttccgtttgc taataaagtc gctgctatta ttggtaaaac
    29581 tgcatttttt gcaccatcta ttttaacact acctttaagt ggattactct tttttactat
    29641 tatcttagcc atattttttg atctcctcat aattaataat ctaattttat aattggtgtg
    29701 gcaatatata tgagtgtttt tccgtcatcc tcattatatt taattcctat atttaaatta
    29761 attttttccc ctaaatattc taattcataa tcatttaata ctttactata tgctgttata
    29821 gataaaaagt ttacgtcttt aacttcatct atttgcttag cactcatatt atataatatt
    29881 ttttgtaaaa tatcatcata tttgttgttt tgtaaccttt ttgtgtattc tccaacaata
    29941 caagcagaca catctacttg agatgaatat ttatttaaca cattttctac gttactataa
    30001 ataacctctt tatgtttata tactttgtta tccaatatgt caactactat gtaactttct
    30061 ttgttatttt tatttattcc ttttatagaa atttctttat cgctaccttt tatagtagca
    30121 cataattgta ttttatcgcc agaccctttt tgattccaat ttattccttt tttatccaac
    30181 tttagattgt taattatttc tataaaaata ttatgaaatt ctttatttga taaattatga
    30241 tttataacag aattaatttt aacattataa aatttaaaat ttgcttctga tatttcaaga
    30301 gctttactta atctattgta tctagtattt atacttgtat ctgcataaga tattgtcatt
    30361 cctataatca ttaatataaa aaagcttata gctaatttta cttttttcat agtaaaaaat
    30421 cccccttaaa tatgtttttc tgtataatag aattattaac aaatttaaga gagatatact
    30481 ttctcaatat gtatttttat acatttttgt atttttcatc taataatctc tttcaaactc
    30541 ttcaatctca attttctcta tccttctcct ggacttgcca atttcttata tgtatcaaat
    30601 acatcttgca ttaattctta tatattctct tctgtaatat aaattactta gtctatactt
    30661 aaatctataa tctataatct attcatattt taccaacaaa ataaacagat aatacttttt
    30721 tatcaatttc catagctcca aatgaacata cctaagtaca cttcccaata cacaatttac
    30781 acactcttcc taataaatca aaaacacctt tgttttaaac aactctattg cactttcaaa
    30841 aactttattt gtgttctccc acttcaatta aatttgaatt atcaagactc cagcccatcc
    30901 atccatcact ataaagggag gtattttcca ctcctatgac atttgcatat gttaatactt
    30961 cagcagcacg ccatccactt ccacacataa atataagctg tttattcaca tctatatttt
    31021 ccttgtccca catttctaaa atctcatatt tatttctcat tgtattatca atattacgat
    31081 attcttctag tgaaacacta tcactacctg aatgtccata taaagctcct ggtatacgcc
    31141 cttttttatc ataatatgta tatccagata cttttcctat atgttcatcc caagtacggt
    31201 tatctactag tataaactta tttttttctt ttagtttttg tcttaattca gatgtagtaa
    31261 caattaattg tgagttaact ggtatatctg ctccaaaatt agtacatgaa tgttttggat
    31321 tactaataaa ttctaattca tatcctgctg ataaccatga gtttgttcca ccattcaaaa
    31381 ctcttacatc ttttactcct atatacctca atataacagc tagtctataa gatgccatag
    31441 gtgtactact tgaaactatt acagtatcat cctttgttaa tccatagtct aaagcaaact
    31501 tagttaagtt atcatcatta tcaagcatcc atgttggagg aggttctata atatcagtat
    31561 ttacatgaac acttgtaggt atatgccctt ttgtatacga ttcttctccc caactcgctt
    31621 caatcatttt tatattcttt gaatcttcaa atgtctcagg aatttttcca tctaatatat
    31681 cttttattat aaaagcagga actatcattt gataattttt atatctttcc attggaagat
    31741 tttcgtcgtc tgcccattgt ttaatatcat atttatatag atatttatat ccttttttag
    31801 ataaataatc tgctacaacc aaagcatctt ttccatttgc atcatacaat actatatttt
    31861 tatctaaatc aattctttta gttttcaatg cttgttctaa cacttcatct tttctatctg
    31921 agtatacact taaccaatta gcagaaaaat ctactgctcc ttttatatgt ccacctcttt
    31981 ttactccatc taacttccag ccattataag catcatttaa gcgagtatca actataaccc
    32041 aagcgttatc ttgcactttt tcttgtaaaa cttcagtaga tattgcctta agattaacaa
    32101 aataatcttc tttcttttct tttttaaaat catttgtcat aatttatact cccaatttaa
    32161 aaaaattttt atttgaaatt attttactaa aataaagtaa ttttcttata ctctattagc
    32221 ttcattatat tcttttataa atagttcctt atattcttat tttaaactac tcactttatc
    32281 ttatcacaag gttactaata ccttgtattt taatttttct cataagctat tattttttta
    32341 tctgatattg actatatgtt tattaagtac tcactatatt ttatatgaac tatatttatc
    32401 gttccctata cctggtatgg tctatatatt tatcatttta cagatgattt aatatcaatc
    32461 tttttataaa atttattata gcaaaaacaa aacaaattta atatttatct aaaaaactca
    32521 tcctataaaa tacaaataac aggatgagtt cttttcaata aatattatct atttaatttt
    32581 taatctgttt ttattcaact gttactgatt tagctagatt tcttggctta tctacatcgc
    32641 atcctcttag tacagagaca tgataagcaa gtaattgcat tggtacaact gctaatattg
    32701 gtgataaaat atcatctgaa tttggtatat agattatttt atcagcagct ttctcaacat
    32761 ccttattatg gctttgagct attgctacta cataagctcc tcttgctctt acttcttcca
    32821 tattagaaac cattttttca aataactttt cttgagtagc tatggcaatt acaggagttc
    32881 ctttttctat taaagctatt gttccatgct ttagctctcc tgctgcaaaa gcttctgcat
    32941 gtatatatga aatctctttt aatttcaatg aaccttccat tgctaagcta tagtctatac
    33001 ctcttcctaa atagaatgca tgttcagaac taacaactgt tttagcaact tcttttatat
    33061 attcttcatt gtctagtatt tcttgtattt ttgatggtat ttctttcatc ttagaaatca
    33121 tactatcata aaattctcta gttattgttc cttttttgat agcaaagtct aaagctatca
    33181 tgtataaaga agttatttga gtagtatatg ccttagttga tgcaactgct acttcaggtc
    33241 ctgcccaagt atagaataca tcatctgatt ctcttgctat agatgaacct acaacgttag
    33301 ttattgaaag tattcttgct cctttagcct ttgaatctct taatactgct agagtatctg
    33361 ctgtctcacc agattgacta actaatatta ctaatgaatt ttcatctaca aatgggtcgc
    33421 tatatctaaa ttctgatgct atatcagtta ttactggtat atttacaaat ttttctatgg
    33481 catacttacc aagtaatcca gcattatatg ctgttccaca agccactata taaactttat
    33541 ttattttttc taagtcttct ttagaaatat ttatactgtc tagaattata tttccattat
    33601 catcaagtct tctttctaaa gtttctctta ctcctgttgg ttgctcatga atttcttttg
    33661 acataaagta gtcatatcca cctttagacg cagcttctac atcccaagtt acatgaaata
    33721 cttctttttc aacaagattt ctattagagt cataaactgt tacattttcg tcttttagat
    33781 gaactacttc tccattttct aagaaataaa catctctagt atattttaat aatgcaggta
    33841 tatctgatgc tataaagttt tcaccttcac caacaccaac tacaagagga ctatcttttc
    33901 ttactgcaac taattcattt ccatgttctt tacatataac acctaatgca taagctcctc
    33961 ttaattttga tatagttttg tatacagcat ctaataaatt accctcatag tatttatcta
    34021 ctaaatgtgc tattacctct gtatctgttt gagattcaaa cttaacacct tcagaaatta
    34081 attcttcttt tatttccata tagttttcta ttatcccatt atgaacaact gctattgttt
    34141 tggcttgatt aaaatgagga tgtgagttta catcagatgg ttctccatga gttgcccatc
    34201 tcgtatgccc tatacctaaa tttccatcaa taggattctt ttctaagtct tctgcaagta
    34261 cagaaagtct acctttaaac tttctaatat taagttcttt ttcattagaa ctattaactg
    34321 ctactcctgc tgagtcataa cctctgtact ctagcttaga aagcccttct actataactt
    34381 ctgctgcctt tctacttcct aaatatccaa ctattccaca cattatttgt gtcctcctaa
    34441 aaatattatt tatatatttt aagagttagg tcatataagt ttactttggc ttccctttgt
    34501 ccataggatt gctcctatta tttaaagaag tctttcgatg gtaaacacac cgcagagcat
    34561 ccgccgacaa ttcgattaac tctgacctcg tcaacttagt tcctatatta ctcaataatt
    34621 atgtctataa ctaagttctg gcgcttagaa aggtgtcctc agtatttata tctgagaata
    34681 ccctttaaat tacgataatt tttcttgtat aagattagct aagtttgttg ctaattcttt
    34741 tatttgacct tcctctttac cctctaacat aactctaact aatggctcag ttcctgatgg
    34801 tcttatcaaa actctaccat ttccgtctaa aatagattct attctttcta tctcagtttt
    34861 tatttctgga tattccatat atttgttctt attttcattt tttattgttg catttactaa
    34921 tacttgtggg tattggctca ttattgatgc aatttctgat aaaggtttct tttcttgcaa
    34981 gattatattt gcaagtatta gagaactaag cactccatct ccagtagtgt tgtaatctaa
    35041 aaatatcata tgtccagatt gctctccacc aagattatat ccatttttaa ccatatcttc
    35101 taaaacatat ctgtctccaa ctgctgtagt agataaattt attccatttt ccttagcagc
    35161 tattgtcaac cctatattac tcataactgt aactaccaaa gtatcttgtg ccaatttatt
    35221 atttttcttt aaatttaagg cactcaatat cattatatgg tctccatcaa caatttgacc
    35281 cttttcattt actgcaatta atctatcagc atctccatca tatgcaagac ctaagtcagc
    35341 attatgttcc aaaacagccc tttgaagttg ttctggatgt gtagagccac atttatagtt
    35401 tatattattt ccatctggtg aactatttat tgaaattaca cttgctccta gttcatcaaa
    35461 cactattggt gccactttat atgacgcacc attagcacag tccaaaacta cttttaatcc
    35521 cttaaagtca gtgcttatta ttgattttag gtagtctaca tagtctcttt gtgcacaatg
    35581 ttcatgtaat tttcttccta cattttcacc aattggaaga caatcaattt tgtctatatc
    35641 gtctatatat tcttctattt tcaattcaat ttcatcatct aatttatatc cattcttatt
    35701 aaagaatttg attccattat attcaacagg attgtgagat gcagatataa ccacaccaca
    35761 atcagcacca tattttctag ttaaatatgc cactgcaggt gttggtatta ttccaacagt
    35821 aataacatca catccaacag acataagtcc tgctattaaa gatgcttcca acatatcacc
    35881 tgatattcta gtatccttac ctactactac ttttactctg tggtctcctt gagctaagac
    35941 aaatccacca gctctaccaa gtttatatgc taaatcacat gttagttctg tatttgctac
    36001 tcctctaact ccatctgttc caaaatactt tctcactaaa gattctcctt tcataattga
    36061 aaagcttgta atttgtatta tatttttata gttcataaaa tatcattata ggcttaattt
    36121 cagtcataaa aatattttaa tacttatcag caaaaaagac aatagaaata attctattgt
    36181 cttttttttg acatcttttt ctttattagt tagttgcttt agaacatgta gcatccatac
    36241 ctttttgtaa agcttctctg tttaatggaa cgaatttttc ttttctaggt ccgaaaactt
    36301 ttttgaatgc ttctagtaca gattctatat ctacagtatc agatactttt aagtaagcgc
    36361 ctaacattat catgttagca actttatcat ttcctaattc agctgctaat tcatttgctg
    36421 gtatataata aacatctaca tctgttctat caactttttc ttttattaaa gaactattta
    36481 ctattatttt tccaccagga ataacatcat ctttgaattt ttcaaatgct ggtatattta
    36541 ataatatagc tgcagttgca tcatctgtta taagtggaga accaactggc tcattagata
    36601 cagttactgc acagtttgct gtacctccac gcatttctgg tccataagat ggtaaccaag
    36661 aaacttcttt tccttctagc attcctgcat aagtaagtaa ttgtcccata gacataacac
    36721 cttggcctcc gaagcctgca catattactc tagctgtaga catttacttc acctcctcaa
    36781 cttcaacatt tttgaaattt cctaatggat agtatggtat catgttgtct cttaaccatt
    36841 gtaaagcatc gtttggagct agtccccagt ttgtaggaca agtagataat acttctacta
    36901 ttccaaatcc taatcctgct tgttgaactt ggaatgcttt ctttatagct tttttagctt
    36961 gtctaacatg agctggagta tcaactgcta ctctttctac aaatacagct cctgttaatg
    37021 tagctaacat ttcacttact cttattggat aaccttgagt ttcagcattt cttcctgatt
    37081 gagcagtagt agctctttgt ccaactaatg tagttggagc catttgtcca ccagtcatac
    37141 cataagttgt attgtttaca aatatagtag ttattttttc tcctctagca gctgcatgaa
    37201 ctatttcagc agtaccaatt gaagctaagt ctccatcacc ttgataagtg aatactgtat
    37261 tttcaggatg aactcttttt attcctgtag cagcagctgg cgctctacca tgagcagctt
    37321 cttgagtgtc acagttaaaa tatttatatc ctaaaactga acatccaact ggaacaacac
    37381 ctacagcatc acctaatact cctagttctt ctaatacttc accaactaat ctatggatta
    37441 taccatgtgt acatcctgga cagtagtgag tttgagtatc ttgtaatcct tcagttttct
    37501 taaatacaac agccattatc tagcacctcc tgcaaccaat tctccggcaa taattttttt
    37561 agctttttca actatttctg ctggtgttgg agtcattcct cctggtcttc catggaagta
    37621 aactggtaat ttaccttcta ctgccatttt aacatcttcc accatttgtc ccatactcat
    37681 ttcaacagtc aatatgtttc tagcattagg aatttggttg aatgcttcga atgggaatgg
    37741 ccataatact ttaggtctta taagacctgc ttttattcct tcttctctta atatatcaat
    37801 tgcgcttttt actactcttg aagtagttcc ataagctgca aatacaaatt cagcatcttc
    37861 tgttttgtac atttcgtatt gtacttcatt cttttccatt gcatcaaatt tttcttgtaa
    37921 atgccaacaa tggtcttcta gtacttctgg ctctaagtat agagagttta ttacattagg
    37981 ttttcttttt cctttagttc caacagttgc ccaatcttta ggaggcaatt ctcttttctt
    38041 ctcaggagct ctaaattcaa ctggctccat catttgacct atcataccat ctgcaactac
    38101 cataactggt gatctgtaat aatctgcaac gtcaaaagct tccattatca tatcaacagc
    38161 ttcttgaact gtagctggtg cgaatactgg agttctgtag tctccatttc ctccacctct
    38221 tgtagacata aagtagtcag cttgtgaagg ttgtatactt ccaagtcctg gaccacctct
    38281 cattacatta agtacaacac aaggaacttc tgctcctact gcatatgtta taccttcttg
    38341 ctttaaagca actcctggtg aagatgaaga agtcataact cttgctcctg cacctgctcc
    38401 accataaacc atgtttatcg cagaaacttc tgattcagct tgaacgaacg ctccacctat
    38461 tttaggtaac tctcttgata aatattctgg taattcactt tgtggagtta ttgggtaacc
    38521 gaagaaatat ttgcaaccag cttctatagc tgcttttcca aatgcttcgt tacctttcat
    38581 aagtatctta gccattaaaa ttccccctta aatattaatt aaattaatct ctttctactg
    38641 ttataactac atctggacac atagttgcac aacttgcaca tcctatgcat ttttccattt
    38701 caaaaacagt tgctgggtta tacccttttt tgtttattac atttgagtct aattgtatga
    38761 tttttactgg acatgcttca acacacaatc cacaaccttt acaacgttct tgattaaaag
    38821 atacttttcc tttagccatt tacctaagcc tccttaaact ttctatatat ttgagctcaa
    38881 actacatcca gtcttctctc atatataatt ttataggaaa aatttctcct tctaaatctt
    38941 ttggtaactg ttcaacaaga ttttctaaac atgcaacata tcttatcggt atattacatt
    39001 tttcagaaac ttcttttgca actttctgac ctcttaaaac atcatcaata gtagtaaacc
    39061 taataagatg agtattatta attagacctg ttacctttaa tttagaagat ttttctattt
    39121 cttttatata ttggataact tcttcagaag tttgagtttt ctccctattt gcatttatta
    39181 caaaaagcat atcataatcg ccttctttta acaaatgact aaatcttcct ataaccctag
    39241 cacctacatt atctccacct aaatctataa cagaattgta tgaatggtct accataggag
    39301 acattacctc tgctgatact gctggtaaat ctaatgttgg agcattaatt gagctatcta
    39361 ttggttgtat acctaaggat ttcattaact ctttcttttc tctagttcta aagtatacat
    39421 ttaccacatc taaatcggct atagcaactt tgccagaaac catatctctt aacttaacaa
    39481 catagttcat cgcaaattca cttttaccac ttccatagtg accagttatg attcttattc
    39541 ttttatcatt agttatcata aacttgagcc tcttcttcac cagttaaaac tctaagtcca
    39601 ccttgagcta atgcaatcat ttcatcttca cctggataaa cttttacatc tgctataaat
    39661 ttaactctat ctgcaatcat ttctgtaaac atttttgaat atgcgattcc accagttaat
    39721 aatattgctt ttacatctcc cttaagaact gcagcactag ctcctatttc tttagagatt
    39781 tgatatgcca tagcttcata tactaattta gctttttcat cgccagcttc aattctttct
    39841 tcaacttctc tagcatcatt agtgtttaag tatgcaacta atccgccatt accttttatt
    39901 ttctttttaa tttcatcttg agtatatttt ccactaaagc acatttttac taatgcacct
    39961 actggtagtc caccacttct ttctggagag aaaggtcctt ctccatctaa tgcgtttgca
    40021 acatctacta ttttaccatt tttatgagct ccaacagaaa ctcctccacc catgtgtgca
    40081 actataagat ttatatcttc atatttcttg tttatttctc tagcatatct tcttgctatt
    40141 gccttttgat ttaaagcatg tactacactt gctctactta tttcaggcat accagaaatt
    40201 ctagcaacat cttctaattc atctacaaca acagggtcta ctatgtatga aggaacattt
    40261 acttcttcac ctatttgttt tgctattatt ccacctaggt ttgaagcgtg ttctcctaaa
    40321 actcccactt ttaaatcttc aatcatagca gcacttactg aataagtacc accttttata
    40381 ggtttaagaa gtcctcctct acctactaca gcatctaatt cagatgtttt tactccacct
    40441 tcttttagag cttcttctat tacttgttta cgaaattcaa attggtcaga caccttctca
    40501 tattttccta tttcttctga agaatgtctt aaagtttttt caaatactaa atcctcatta
    40561 tcaaatacag ctattttagt tgatgtcgaa ccaggattta ttgttaagat tttaaatatt
    40621 ttgctcattt gcctatttcc cccttaaaat tatgcctttg ctgccattaa aacacctaaa
    40681 gctattgagt ttagtttagt ttcttcactg tctgctctag aagttaatat tattggtgct
    40741 ttagccccaa ctataactcc tgcatttttt gattttgaga agaataccaa agctttatat
    40801 aatatgttac caccttcaat atctggggct aataatatat cagctcttcc tgctacagga
    40861 tgatttatac ctttatgttt agctgcttct aaagatactg cattatcaat tgcaaaaggt
    40921 ccaccaacca tacaaccttt gatttctcct ctttcataca tttcttctag ttctttagct
    40981 tcaactgtat ctttcatttt tggatttact ttttcttttg cgcatattgc agcaactttt
    41041 ggttcactta tatctaatga atgtgctact gtgcaagcat tttctatgat ttgcttttta
    41101 gtatttgtat caggagctaa gttcatagct gcgtcagtta cgaaaaataa tctatcatat
    41161 ccctctacat caaatactgc tacgtgactt aatacatttc cagttctaag acctacttct
    41221 ttatttaaaa ctgcttttag tattattgat gtgtctacta agcctttcat taccatgtcg
    41281 gcttttcctt gtgaaactaa ttcaacagat tttagagatg cttctgctaa atcttttata
    41341 tctatcagtt cataattttc gatatccatg tctatgcttt ttgcaatttc tttagtcttt
    41401 tctatatctc ctactaaaat ggcatttgct attttttctt ttctagccat ttcaactgcc
    41461 attaaaactt ctttatcttg gcaacatgct actgatatag ttttaggtcc tctttctttt
    41521 gcaaacttaa ttacttcttc aaaacttctc atgcatttcc acctcatctt gaattttttg
    41581 aaatagaact ttttatatct caattaaatt ataactcgtt cagcaacgtt ttcctagaga
    41641 taattgaaat atttaattca aaatatttat ttaaatctat ttatcagtct tttttcatat
    41701 atctagcgct ttttagattt aacaaatatc tcattgtcct tattttataa tataactaaa
    41761 ataaacaaga tgcaacaaaa acataaaatt aacaaactta attttatagg tttttttgtc
    41821 gcatcttgta acaactaaat attcgaaata tttgttcatt ccacttcaac aatattaggt
    41881 tctatattta tttttttaaa ttgatatgtt gtttcatatt ttattttata tttactatct
    41941 tcctcaggac cttgtgataa atcaatatat aatgaaatat cttttttatt taacttactc
    42001 aaatcctccg ttgactctac aacaactttt atatctacat cagaaattac atttggatta
    42061 tccatatttg ttgggatatt tcttaattca atatctcccg cattatataa aaattctgca
    42121 ctcaattgtt ttgtatcatt taatttaatt gttatagagc tatcctcaag agttataccc
    42181 tctggaattt ctaagctaac ttctttttct tcaccattag ccaaatcagc taagtcaata
    42241 ggttttgtct taatataact tattttgtct atatcatctt tctttccttt tattgtaact
    42301 tgttccttac ttaatttata gtttttcagt acatcattat ctttattatc atttagactt
    42361 actcttattg ggacaatttt ttccttcaat agctctatct ttgcagtcac aaaggatgaa
    42421 cttaatgtta ctccttcaac cttagttcca tcactattta atggtattaa ttgcaatttt
    42481 ttagaaaaat catctgtctc attaccaaca tctagattag ctcttacact tttgacttct
    42541 ttaactagag ttcttggacc tgatacatcg acaaattcct tactaagttc aattttattt
    42601 atatttcttt ttgaatttcc acttacttct acctttatat ctcttttttc ttttatgttt
    42661 ttttcaagat ttactactaa aacatcatgc ttaaattcta gagtaacacc ttgaggaata
    42721 tttgctttta aatatacttc cttctgtcct tgtattgggt cttcaatggt tccatataca
    42781 tgtatatctt cttttttaac actttgtacc tttgataaac taccttttat atatacatct
    42841 gctgttaatt tttcgtctgg atatataaca agatttttat cctttaaatc actcatatta
    42901 cttatagata caggtatatc ttcaaaccct ttagtttctt cagggtcaac tacagccata
    42961 acatacatcc ataatgcaat tgcacttaaa aatgatatta tttttatttt ggtattattt
    43021 tttagtctat ttatcattta aatataccac ctttgaaaaa acttcttgtt tctgtatttg
    43081 tttttaaatt actacgtaat atattcatca ttctttctct agaaatatct ctatataact
    43141 tacctgcttt agcaattgat acgccacctg tttcctctga tacaatcaga gttatacagt
    43201 cagacacctc acttacccct attcctgctc tatgtctagt gcccaaatct ttacttaaat
    43261 ccttactttc tgtcaatggc aaaaaacatg cagctgcttt taccttagaa tctctaatta
    43321 caacagctcc atcgtgtaat ggtgtatttg gtataaatat atttattaat agttgcctag
    43381 aaatttcagc atcaataata gttcctgtat ttattatatc acttattcta gtttctctct
    43441 ccattatgat aagagctcct attttttgtc tagaaagtga atataaagct tctacaattt
    43501 cttctatatt tctattgaga gtttcttcag aagtatttac attctttcca aataaattaa
    43561 actttgctct tcctatatgt tcaagtcctg ctctaaattc tggttgaaaa attataagag
    43621 cagctataac tccataatct aaagctttaa gagatatcca ataaacggta tgcaatttaa
    43681 aggtgttact aagttgagtt gctaataaaa gaaatattat acctttaaac acttgttctg
    43741 ctcttgtatc ttttataaac ataaatattt tgtaaaaaat atacgctact atagatatgt
    43801 caattaaatc atatatactc attcttaata ttatattaaa aaatccattt aaaaaagaat
    43861 ttatatctaa cacagtttta tcctcctgtt tgataacttc ttaat
//
LOCUS       DBGJFF010000018        43744 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.018, whole genome shotgun sequence.
ACCESSION   DBGJFF010000018 DBGJFF010000000
VERSION     DBGJFF010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 43744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43744
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.018"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            <1..257
                     /locus_tag="ACOIAF_001035"
     CDS             <1..257
                     /locus_tag="ACOIAF_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HGZ2668056.1"
                     /translation="ILTLQAFLYMAISPIPTPGNVGANELAFFTIFKSVFPQPLMGYA
                     VFLYGGFMYYLILIGSGIFTVITHYRMKNRIGRNIVLSEN"
     gene            508..2652
                     /gene="fdhF"
                     /locus_tag="ACOIAF_001036"
     CDS             508..2652
                     /gene="fdhF"
                     /locus_tag="ACOIAF_001036"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:922..924,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HGZ2668057.1"
                     /translation="MEKKILTVCPYCGAGCQLYLVVKDNKILRAEPANGRTNQESLCL
                     KGRYGWDFLNDPKILTPRLKKPMIRKQGVLEEVEWDEAINYTASRLNEIKEKYGPDSI
                     MGTGSARGPGNEANYIMQKFMRATIGTNNVDHCARVUHAPSVAGLAYSLGSGAMSNSI
                     PEIENADVLFIFGYNGADSHPIVANRIVKAKKNGAKLIVTDPRVTESARIADIHLPIK
                     GGTNMVLVNAFGNVLIEEGLYNKEFVQNHTQGFDEYKEIVKPYTAKYAEKITGIPEEL
                     IRKAMREYAKGKKAMILYGMGVCQFGQAVDVVKGLASIALLTGNFGRESVGIGPVRGQ
                     NNVQGACDMGALPNVYPGYQNVTDDKIREKFEKAWGVKLSPNNGYSLTQVPDLVLKEK
                     KLKAYYIFGEDPVQSDPDASEVREALDELEFVIVQDIFMNKTALHADVILPATSWGEH
                     EGVYTCADRGFQLMRKAIEPQGDVKPDWQIISEISTAMGYPMNYKNTKEIWDELRQLC
                     PSFLGATYEKIETQGCVQWPCKSESMEDKGTMYLYEGQKFSTPNGKGNLFAAEWRPPM
                     EVEDDEYPFSLCTVREVGHYSVRTMTGNCRTLSSLEDEPGRVQINSNDAEKLGIEDDE
                     LVRISSRRGSVITRATVTDRVKEGATYMTYQWWVGACNELTIANLDPISKTPEYKYCA
                     VKLEKLEDQELAEKCVREEYQSLKDKMTATNI"
     gene            2701..3534
                     /gene="fdhD"
                     /locus_tag="ACOIAF_001037"
     CDS             2701..3534
                     /gene="fdhD"
                     /locus_tag="ACOIAF_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HGZ2668058.1"
                     /translation="MNNIYSKTKLQENSKDKVFENSYSLVDYKYLNSFNVEVSKINEH
                     EVSIDNEEIIAEYPLSFILNDKYINTFLCTPYNLEELIVGFLKTKGYIENKKSLLKFE
                     INESERFAKVTIQEKQKNQDEQIIFLNSLDYITCAPVKSNIKINYDTIYSIMDKNLNS
                     SELFKNTGGVHSVSVFNFDKKIVTCEDVARHNAMDKAIGFCVLNDITLKDKIVAVSGR
                     ISLEMILKAAMTQIPIVVSKSAPTNLSIELSKKLNITLIGFVRGRRMNIYANPQRVIK
                     K"
     gene            3545..3718
                     /locus_tag="ACOIAF_001038"
     CDS             3545..3718
                     /locus_tag="ACOIAF_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668059.1"
                     /translation="MCTKKEEQRYSKCEICGVLIDDTDKVYNNTYNLCKKCKRNISTE
                     KIVKYINIYDENN"
     gene            3755..4306
                     /locus_tag="ACOIAF_001039"
     CDS             3755..4306
                     /locus_tag="ACOIAF_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HGZ2668060.1"
                     /translation="MKNKIGSFVIADPNKCTGCRACEVACFTMHNQHNNVGYTVGTVD
                     IPVIPRLYLVKGDNFCMPIQCRHCEDAPCLNSCPQKAIVKENNIMSVNEEKCIGCKTC
                     LLACPFGAIDLLPQYEDGKEVEQVILDENKKIAYKCDLCKDNEKIACISACPQEALKL
                     VTPIDDKKAKNRQAALSLLLTNK"
     gene            4331..5707
                     /locus_tag="ACOIAF_001040"
     CDS             4331..5707
                     /locus_tag="ACOIAF_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016695 - oxidoreductase activity, acting
                     on hydrogen as donor [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase, group A"
                     /protein_id="HGZ2668061.1"
                     /translation="MIINIDKDLCTGCRECSKVCPVNAIEGEEGKPQEINLDRCVMCG
                     QCVQTCKSYASVIDEGFEFLQEKKQEREIPESINEPIFAAYNVCDIDKVKKALSDPNV
                     FTMVQCAPAVRVALGEDFGYYLGYLGAGKMAAALRALNFDRVYNTNFAADLTIMEEGS
                     ELIKRVTEGGTLPMFTSCCPAWVKFMEKNYPKLTNHLSSCKSPQQMGGAIFKTYGAEI
                     NNVDASKIFSVSIMPCTCKKYECDREEMDSSGYRDVDVVLTTRELAYLIKDMGIDFKN
                     LREEKFDSPLGSYSGAGTIFGVTGGVMEAAIRTGYELITGESIPDVEVKQVRGENGFR
                     KSTIKIGDLDLRVGVVSGLKNVIPVLEDLEKGKLDVDFIEVMTCPVGCVSGGGQPKIL
                     LEEYKELAYENRTKATYVHDEHLAIRKSHENPDIKKLYDEYLVEPLGEKSHHLLHTTY
                     CIGKEVAK"
     gene            5707..6222
                     /locus_tag="ACOIAF_001041"
     CDS             5707..6222
                     /locus_tag="ACOIAF_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HGZ2668062.1"
                     /translation="MNYFVIADPDKCIGCRTCMIGCVVAHSDEDIFYQNPDEINFNPK
                     LSVIKTKEVSAPIQCRHCEDAPCAKACPNGGIVRVGNTIKINEENCIGCKTCMLACPI
                     GAIDIVTLKDVDEGKLCFRERMTANKCDFCIESPEGPACVNVCPTKAFTIVKDEDIDS
                     KVKNKRKLAIL"
     gene            complement(6300..7583)
                     /locus_tag="ACOIAF_001042"
     CDS             complement(6300..7583)
                     /locus_tag="ACOIAF_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugated bile salt MFS transporter"
                     /protein_id="HGZ2668063.1"
                     /translation="MNTLLNNKFSIKNFSNAWVIVLGCMLIQAVPFSIVASIQPQFIS
                     YVVEGEGFSLASFSLIFTIGTIASAIASPFIGVLFSKVNIKILYLLGCIISSIAFATF
                     SICTELWQFYTVSCIVQIGATIISAIGIPVLISAWFDEESKGKALGFAFAGGSIGSIF
                     LQPLAVRLLATQGYSRSYLIFGIIALIVGIPVILFILRMPKDINEKVKSKGKKEKASD
                     TISPSVWGYTLKEAKKIKYFWIMTIGFIFVGIYVSSLSVQYPTYLRNYLNPILIGTVG
                     STFALFALIGNLFGGLLFDKFGQTKPLIISFVLVLIATLSLLLVDKSSTFAFVFAILK
                     GLSVFAYMIGPAYLTGSFFGQKEFGSIYGIVQLIFAVGFALGSTLFGLLVDNFGYRFS
                     WSIIILSVIIAYTLLIITSIGMKKLNKERLDKLMQ"
     gene            7771..8193
                     /locus_tag="ACOIAF_001043"
     CDS             7771..8193
                     /locus_tag="ACOIAF_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3783 domain-containing protein"
                     /protein_id="HGZ2668064.1"
                     /translation="MSFEKINNNNLDKIHNRKCIMLVNFNKKELSLIKNVSNFAGIRD
                     HIILSDKNGENIIRDILDGNISNECENGIKSKSVIFNNTPSTQVNAFLEGLKKVRINR
                     PFTAMVTETSIDWTLNNLIENLVQERNAMSLGKTIEHK"
     gene            8295..9233
                     /locus_tag="ACOIAF_001044"
     CDS             8295..9233
                     /locus_tag="ACOIAF_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HGZ2668065.1"
                     /translation="MKVLFTINYGKEKFDKIRELGYNVIHYSEDSIENNEDVNTADVL
                     VTYNPFERLDISKMKNLQYIQTTSVGIDQLPKEEVLKRNIKIANNRGGYSVPIGESIV
                     LYILEIYKNSAKFFKQQQNKEWKMDFSVSELYGQRVGFLGTGTISSEAAKRLKAFGVE
                     IWGVNTDGSSKKYFDKCFATEDMNIVFEECDIVVVAMPSTKSTDGIVNKDMFKLMKDG
                     SVFINVGRGNTVNQKDLEECVEKFRGVALDVFESEPLNKDNKLWECENVIVTPHNSWV
                     SDKNKERTFNMVYNNLKHYIMENKPVDNVVDISRGY"
     gene            9419..11443
                     /gene="ligA"
                     /locus_tag="ACOIAF_001045"
     CDS             9419..11443
                     /gene="ligA"
                     /locus_tag="ACOIAF_001045"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HGZ2668066.1"
                     /translation="MSVKDEINKLIETINYHNERYYNQDSPEISDYEYDKLMKELISL
                     EEEHPELKRIDSPTNRVGGKPLDKFNQIVHKTPMLSLSNAFSEQDLRDFDKRVQEYVG
                     ENVEYVVEFKIDGLSVGLTYNNGEFEKGATRGDGVVGEDISQNLMTVKSIPLKINDKK
                     ELIVRGEVYISKDNFRKVNEQQEEQEQPLFANPRNLAAGSLRQLDPKLTAKRPLDIFV
                     FNMENIEEYDLESHSESLEYLEKLGFSVSQSYKVCKSIDEVIEHIEYWTDNRGNLDYE
                     IDGMVIKVNNIKQRDEMGYTAKSPRWAIAYKFPAEKKKTKIVDIIVEVGRTGTITPSA
                     ILEPVRLAGTTVSRATLHNEDYIREKDIKINDTVLVQKAGDIIPQVLEVIKEERTGDE
                     IDFKMPEKCPVCSEPTIRLEGEAAVKCINMSCPAQIRRGIIHFVSRDAMNIDGLGESI
                     ITLLLNEKIIQDVSDLYYIKKEDVVDLERMGEKSADNLINSIEKSKENDLWRLINGLG
                     IKFIGVKAAKILAYNFKDLDEVISATSEQLEELEEFGSIMANSVVEFFKEDKNMNVIN
                     KLKNVGVNTKSLDNTGEKVEKIFEGMKIVLTGTLPTLKRNDAKEMIEKRGGKATSSVS
                     KSTTFVLAGEEAGSKLTKANDLGIKVIDEERFLELLKLSSKDEVKAVLES"
     gene            11603..12952
                     /gene="rph"
                     /locus_tag="ACOIAF_001046"
     CDS             11603..12952
                     /gene="rph"
                     /locus_tag="ACOIAF_001046"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HGZ2668067.1"
                     /translation="MTRSYDRSNEQIRPVKITRDYTKYAEGSVLIEMGETKVICTASV
                     EEKVPPFLRNSGTGWINAEYSMLPRATQQRKIRDASRGKIDGRTQEIQRLIGRAIRSV
                     VDLNKLGERTIWVDCDVIQADGGTRTASITGAFVAVAEAIYKLYKAKTIKEMPITNFV
                     SAISVGIVNGIHMLDLCYEEDSKAQVDMNIIMTDKCEFVEVQGTGEEKPFSRADLNLL
                     LELGEKGNKELIRLQREALGEISDEILGMDYGNEVVIATNNVHKLEEIGEILKDFEYK
                     VYSLKDVDLAGIEIVEDGKTFEHNALIKARTIAKKTKLIAISDDSGLEVDALGKKPGV
                     YSARYAGEHATDEENRKKLLKAMQNVPMSKRNARFVSAIAVVFPDGKEFVVRGICEGM
                     IGFEEKGKNGFGYDSLFIVKGYDKTFGEIPSVIKNSISHRANALKLMKQEFIKRVVK"
     gene            12952..13422
                     /locus_tag="ACOIAF_001047"
     CDS             12952..13422
                     /locus_tag="ACOIAF_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HGZ2668068.1"
                     /translation="MKIGIVSDTHMMIKNMEKTIPYLKECDLIIHAGDNFTDSRYIHS
                     MTNVGIIAVKGNCDFDAVEEEVVFEVANKTIFLCHGDKYGVKYGTNMLEKKATEVDAD
                     IVIFGHTHTPFREIKDGVLYINPGSTSLPRGVSYKSFVIMDIEEDDIKIEEIRI"
     gene            13616..14902
                     /gene="tig"
                     /locus_tag="ACOIAF_001048"
     CDS             13616..14902
                     /gene="tig"
                     /locus_tag="ACOIAF_001048"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HGZ2668069.1"
                     /translation="MKAELIKKEGNKVTLKITVDNNKFEAAVNKAYNKSRNKYNIPGF
                     RKGKAPRIVIETQYGKGIFYNDAIEILFPEVYPEAIKELDIDPIDNPDLDIEEISKDN
                     GLVMVLNVEVKPEFELGNYKGIEIAKVENTVSDENVDAKLQEMVEKNARLVSVEDKAL
                     EDGDTAIIDFEGFENGVAFDGGKGENYNLVIGSNTFIPGFEEQLLGKKAGEEVEVNVT
                     FPEEYHSQDLAGKPVVFNVKINDVKVKELSALDDEFAKDTSEFDSLDELKADVRAKLE
                     EEAKNRADAETRNSVVEKVAENTEIEIPEVMIQHQIDNMLNELNYQLQYQGFGLQQLL
                     EMTGRTMEELREERKEDAKKLVKSSLVLEAITKAEGIEATEEEFKAELEKMASAYNME
                     VEKIEASLRDADKEDIKGQIKIRKTIDLLVDNATIA"
     gene            15052..15636
                     /gene="clpP"
                     /locus_tag="ACOIAF_001049"
     CDS             15052..15636
                     /gene="clpP"
                     /locus_tag="ACOIAF_001049"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HGZ2668070.1"
                     /translation="MALVPVVVEQTGRGERSYDIFSRLLKDRIIFLGDQVNDATAGLI
                     VAQLLFLEAEDPDKDIHLYINSPGGSITSGMAIYDTMQYIKPDVSTICIGMAASMGAF
                     LLAAGAKGKRLALPNSEIMIHQPLGGAQGQATDIEIHAKRILKIKETLNEIFSERTGQ
                     PLEKIKMDTERDNFMSALEAKEYGLIDEVFTKRP"
     gene            15655..16905
                     /gene="clpX"
                     /locus_tag="ACOIAF_001050"
     CDS             15655..16905
                     /gene="clpX"
                     /locus_tag="ACOIAF_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HGZ2668071.1"
                     /translation="MSKYEEKRQLKCSFCGKNQDQVRRLIAGPNVYICDECVELCDEI
                     IQEEIEDTIDEDTTSLPKPKEMMEILNDYVIGQEKAKKALSVAVYNHYKRIYSKKSSS
                     KDIEIQKSNILLLGPTGSGKTLLAQTLARTLNVPFAMADATSLTEAGYVGEDVENILL
                     KLIQAADFDIEKAERGIIYIDEIDKIARKSENPSITRDVSGEGVQQALLKILEGTVAN
                     VPPQGGRKHPHQEFLKIDTTNVLFILGGAFDGLEKIIQKRGGDKTLGFGAKIESKKEL
                     DLGKLYEKVLPEDLLKYGIIPEFIGRIPVLATLELLDEDALMQILQEPKNALVKQYKK
                     LLELDDVELEFEEGALRAIAKKAIERNTGARGLRSIVESVMMETMFEVPSRDNIKKVI
                     VTEKSVNEDSVNPIIVLKDQEESA"
     gene            complement(16976..17503)
                     /locus_tag="ACOIAF_001051"
     CDS             complement(16976..17503)
                     /locus_tag="ACOIAF_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HGZ2668072.1"
                     /translation="MSEIILLFLIFLKIGAFSFGGGYAMLPFIQQEFISKYHFITNQE
                     FLDLLAISQSTPGPVAINSATFVGFKTAGFFGSLAATIGVTIFALIGLTIISKLFSKF
                     KNNKKVENLLTVLRPITLGFILSAAFSSAKEVNWDLYGIIAFIASFYLLYKGKIGSIA
                     IVFVFGILGILLTFL"
     gene            complement(17491..18003)
                     /locus_tag="ACOIAF_001052"
     CDS             complement(17491..18003)
                     /locus_tag="ACOIAF_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HGZ2668073.1"
                     /translation="MLKLFLTFLKIGAFTFGGGYAMVPLIQDELVNKQKLLEQEEFID
                     YLSIAQSYPGVLAVNISVLLGYKLYGIPGVLICTLGSALPSFCIVLVLSYLYFKNSSS
                     KILDGFFKGVAPVVIALILYSFTSMFKKLPKSKTNILLLIIAVLSVGVFNISPIYLII
                     GGGVYSLCQK"
     gene            18183..20546
                     /gene="lon"
                     /locus_tag="ACOIAF_001053"
     CDS             18183..20546
                     /gene="lon"
                     /locus_tag="ACOIAF_001053"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HGZ2668074.1"
                     /translation="MEQNYTKIDHELPLIPLRGLAIFPYMILNFDIGREISLKALDQA
                     MMDEELIFLTSQKEAEVDEPGEEDFYHVGTICKVKQMIKLPGDTVRVLVEGVSRGRVK
                     KIEQEDGYFRAVIEEIVFDSDNLDSETEVEIEAFVRNVFDAFEEYINIGNRVSPEILI
                     SLADIEDVDRFIDTIAANIYLKSSQKQEILEEFDIRKRLELIYSILLEEIDILKIEKK
                     ITLRVKKQMNKVQKEYYLREQLKAIQKELGEEEDINSEADEYREKLKKIKAPKTTKEK
                     IEKEIDKFSKISSMSPDVSVSRNYLDTIFSLPWNKETKDKLDITKAKDILDEDHYGLE
                     KVKERILEYLAIRTLAKSLKGPIICLVGPPGTGKTSIVKSIARALNRKFVRISLGGVR
                     DEAEIRGHRRTYVGSIPGRIINGVKEAQTKNPVFLFDEIDKMAADYKGDPASAMLEVL
                     DPEQNKDFVDHYLEIPFDLSKILFVTTANSLGNIPRPLLDRMEVIEVSGYIEEEKLNI
                     AKKYLLPKQIKEHALKESFIKIDDETLRSIINHYTREAGVRTLERTIGKICRKVAKKY
                     VEDPTLEEVVINKSDLETYLGKDMFKYQLAEVNPQIGLVNGLAWTEVGGVTLEVEVNV
                     LKGKGEIVLTGKLGDVMKESAKTGISYIRSIVDKFDIDPEFYKTNDIHIHIPEGAVPK
                     DGPSAGITMALAVISALTKRPVPGNIAMTGEITLRGRVLAVGGIKEKLLAAHRAGITK
                     VLIPKECEADLDEIPENVKEKMEFVLVEHMDEVLEQALLKSGENNEN"
     gene            20536..21147
                     /gene="yihA"
                     /locus_tag="ACOIAF_001054"
     CDS             20536..21147
                     /gene="yihA"
                     /locus_tag="ACOIAF_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HGZ2668075.1"
                     /translation="MKIRSSEITMSAVNKSQYPAEGIPEIALAGRSNVGKSSIINTLL
                     NRRNFARTSQTPGKTRTINFYLINNEFYFVDLPGYGYAKIAKSEKEKWGGIMERYLES
                     RQELCSIFLLVDIRHEPTADDKLMYEWIKHFGYNCVVIATKADKISRGQYQKHISIIR
                     KKLQMESSEKVIPVSSLKKTGVEELWEEIVNQYNQHGYEITVD"
     gene            complement(21412..22782)
                     /locus_tag="ACOIAF_001055"
     CDS             complement(21412..22782)
                     /locus_tag="ACOIAF_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGZ2668076.1"
                     /translation="MKQKWIVLIIICIGVFMSTLDGSILNIANPTIAADFKINMSQIQ
                     WVVTAYMLVVTATMLFFGKLGDKVGSNRLYTLGFFIFTIGSFLCSMSNNLSTLISSRI
                     FQAVGASILMATGLGIVSNAFPANEKGKAIGITGAVVGIGNMSGPVIGGIILEHFGWP
                     SIFIINIPIGIIAVFLGIKFLPKPVLDEQNKSFDIPGLLLFASCTTLILLAMNEKGNT
                     RLYLGITALIIFLLLALREVKFEQSFIDLPLFKNRNFTVGNIIGVACYFPQMAVSFLL
                     PFYLEQLKNLSPMMAGYVMTVHPLIMVLIAPIAGSLSDKHGAKNILTASFSFMTISLV
                     GMALLKADSPLYLLIVCLVIFGLGLGAFSSPNNSSILADVPPQKQGYGGSFLATIRNL
                     SFALGTAFFSSFFAQSLTYNQKFKSHTSAYVIASNQSYWIAASVCFIGLILTVFFMRK
                     TDKSIS"
     regulatory      complement(22957..23072)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            23360..24439
                     /locus_tag="ACOIAF_001056"
     CDS             23360..24439
                     /locus_tag="ACOIAF_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGZ2668077.1"
                     /translation="MEGKVKKAFPGGNTSQGSYSFFDYIIPENVKRVFCLKGGPGVGK
                     SSLMKKIYKEFLHRGYDIDLYHCPSDPSSLDGLVIKKLGVVLMDATAPHTMDPKLPGA
                     LDEIINLGDYWNTEELEKNKQEISVYDKDISNSFKRAFKFLKSAEPIFRDIEEKYSDC
                     MDYGKVNLVTEEFIKRLFDGVKSTGNLKREKHLFGSAITPVGCLDYTENILKDVKKVY
                     YLDGEIGTGKTTLLNKVYKKAIEKGLNVEVYHYPLIPEKIETVLLTDLDIGITISTTF
                     KEEDTIDLNQFLNREKLLKYEEDIKFDEKVLDDLICWAVLNLKRAKSKHDIIESYYSP
                     NMRFDEVGKLRDKLIKRILKYEENL"
     gene            24830..25510
                     /locus_tag="ACOIAF_001057"
     CDS             24830..25510
                     /locus_tag="ACOIAF_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HGZ2668078.1"
                     /translation="MKKQYVFIILASTILGILVGTIFRDYSNEETVYTSKDKSIRKEI
                     KMTRKSIKELTKEKDKLDKEINSMKQNQKNSEEISEIETLRDSLSYSDVKGSGIILSI
                     DAIDEKTGNIANSIDYNKILVKVINELKVNGGKYIEINGQRINQYSEVVLAGSHININ
                     GVPVAQPYEIKCIGDKEKLSNYVDKGSKYLDSIQTNYPMKISIKTEENISMKKMNIPN
                     KLKYIKGD"
     gene            25503..26882
                     /locus_tag="ACOIAF_001058"
     CDS             25503..26882
                     /locus_tag="ACOIAF_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HGZ2668079.1"
                     /translation="MIKIILEESSLPMLIPEKLAKKYNVFPVEFQDDNLVVEIEDEDI
                     YALQDLRLATGKEIILKKEKKELISEKIEKYYNSFELDEDYAKKLFENLLEKAIKENA
                     SDIHIEPFKEHLIVRMRVDGELKEVSKFLVDVYPSLSTVIKLKASMDITEKRLPQDGR
                     VDIKLGGNLIDIRVSSIPTVYGEKIVLRILNRDTFLKDKLELGFSEEAIKSINRIINK
                     RAGILLVTGPTGSGKTTTVYSLLNDLKGINKNIMTIENPVEYKMDGISQIQVNSKVGL
                     SFDVGLRAILRQDPDIIMVGEIRDAETAKIAVRAAITGHLVISTLHTNDAVSSIARLL
                     EMQIPPYLLNASLIGVISQKLVRKVCNHCSHEIMIKDNFSGDVNTKVAVGCEKCNDKG
                     YFGRTAIYEILEINDDIKTCIRNMEDSSTIKDVAQNNGMITFEDSCKRLINEKITTLE
                     ECLVVNEMI"
     gene            27053..28138
                     /locus_tag="ACOIAF_001059"
     CDS             27053..28138
                     /locus_tag="ACOIAF_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HGZ2668080.1"
                     /translation="MRLSKNIFYNLEKFYLVLNRISDKEIRVICKEIGILLESGCEIT
                     KIFEIIESQSSKKVKNLLSIVSNHIQKGNSIAESFQITGIFSKFFISMIKAGETSGNL
                     DIIMSDLSNYYDKEYKLKMKIITISIYPIILIILSILSMLFIFVFVIPNFQVVFTNNG
                     IEPPLITRVLMGISTVVTNNLAYIIFSFILFSIGSIYFFITNDNIKKLINSLKFKIPF
                     IKKINQLVATTRFSRALSVLISSGIQIVEAIDMSASVIDNEFIYERLKISNNYIKKGN
                     SIGKSLNLANVFPKLFISMANTGEESGKLDKSLNVINKFYENELNNKIEQIMKFIEPM
                     LIVFMGLIIGTFILAMVVPMFDAITNF"
     gene            28192..28551
                     /locus_tag="ACOIAF_001060"
     CDS             28192..28551
                     /locus_tag="ACOIAF_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HGZ2668081.1"
                     /translation="MINLINKKRKGFTLVEMIVVVTILGVISSIALVKYSKVQESAKL
                     NADYTNAANIVTAASMAINDDEKTIDSLSVETLKEKGYLNTVPVPQSTSGKFELVIND
                     SGTDISVNINSKQFYPK"
     gene            28604..29476
                     /locus_tag="ACOIAF_001061"
     CDS             28604..29476
                     /locus_tag="ACOIAF_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HGZ2668082.1"
                     /translation="MAIIKKILNLEINDDYIRLVFLRKGHKKVFIDKSIIKEINFDIK
                     NDSNSIEKIKYIEDVVRFVKEEIGKSKILYKNLYFNIQIQDIVIRNVEVLNTKKKRDI
                     LSMIEFEITQYIPININNYSIKYKVINSKNDKLNIQVILFPKSIIQLIKNISENLNMN
                     PKTININFDILQKLINLNLIENFSEEGVFIECKKSQLIINITKDKKIRETYLLSKEGK
                     SYEFIKRLLRDFKYVYYYGIENCFIEEYFKYTDKSIEVNPLRLKDKVKVENNFDADLE
                     DNRINYISNIGMII"
     gene            29477..29920
                     /locus_tag="ACOIAF_001062"
     CDS             29477..29920
                     /locus_tag="ACOIAF_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668083.1"
                     /translation="MKVDKTINFLNGFKKTKPCNKIYKIIIVLILTCILFKSFMYVKS
                     INELENYISQNKLVDKSKNTLPTTDKTREKFSKIKLNEIKKIYETIGINSITKISIDK
                     NKLEMEGVCSDLDVLNNLRKIKYIKYYYIDSIVRDGEYYRFRIKS"
     gene            29935..30519
                     /locus_tag="ACOIAF_001063"
     CDS             29935..30519
                     /locus_tag="ACOIAF_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668084.1"
                     /translation="MRLNNREKNLLKILLILVIILASDIAVSYLEENLTSLKNEKQNI
                     INKINNQDNYQIKDNKHIDDKYIESVESNKNEELKNNFNNSFLDKKTNKDIVLLVENE
                     LRDIVNVEYINQESSLDNHNKKQVNLEIKVNGELANVLKLEGVMKKLRLNNKLEKIEI
                     NKLKVNNFDVQNIPNEYKQEKDNIVECKMTVRVI"
     gene            30772..31809
                     /locus_tag="ACOIAF_001064"
     CDS             30772..31809
                     /locus_tag="ACOIAF_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HGZ2668085.1"
                     /translation="MLGRNELCPCGSGKKYKKCCLNKDIVSERACRKTELSQKQYTDL
                     YSKLYDYSRQDKFKEECEKAKEMFYIMQNDSVNEKFERFFNTYLIQDHIMENKKVMTV
                     GFLEENGANLSQSEVSILKSMFESYVSIYEVKEVSAGKIVLKDCLSGNELCTEDVKLL
                     RSFKIGSCMIARIVDIEGTNILIDITISISNEVKDIILKDIMNLFNQYQDVYKDMKSF
                     LIYHTHILYKYIQQLLDPSIAEYLKRERDKNDTKVNSENELAEEECKVLDTLKENVEK
                     ENIDACIEFWNEYKKNHENIKGAENGWAAAVEYYVKKEAGQPITQVQISKKYDISSST
                     LGKRYKDLKIS"
     gene            31890..32255
                     /locus_tag="ACOIAF_001065"
     CDS             31890..32255
                     /locus_tag="ACOIAF_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668086.1"
                     /translation="MSLSLGNAIAVSTNFIKSNPAVPVYNGEEIYAVIYHIEIGYNNG
                     VSPDTKQYIIIDYKEFDLSMDKKNELINIAKEYCKSKGIVENENLEINLLDNEEAENF
                     LEKVFNNMKVIRGLITKKG"
     gene            32260..32577
                     /locus_tag="ACOIAF_001066"
     CDS             32260..32577
                     /locus_tag="ACOIAF_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668087.1"
                     /translation="MDYIGIENITPYENTYEFSVYEYDDEITLGSEKLYVCELRVVLI
                     KVNSLYVERLHKSVEAMVLVKNLKKDLDKTLVVNKIKNFVLDEIWVENLVKENIEVIF
                     VES"
     gene            32625..34229
                     /locus_tag="ACOIAF_001067"
     CDS             32625..34229
                     /locus_tag="ACOIAF_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HGZ2668088.1"
                     /translation="MLRVSNVKLGIDKDISLLKDVILKKLKIKEKNLVKYSIYKESID
                     ARKKGKMEFVYSVDVEVKDENKILNSKIKDVTKIKEMRYVNVPVGDKKLKNNPLVIGS
                     GPAGLFAGLLLAQMGYEPIILERGLDVDSRTKDISDFWTTRKLKNNSNVQFGEGGAGT
                     FSDGKLTTRIKDIRCRKVLEELVQFGAPDEILYSHKPHVGTDILKNVVKNIRNEIISL
                     GGQVRFNSKVTDIVVSNDSIQSVIINDTEKIDTDTIILAVGHSARDTYEMLYSRGIKI
                     VQKPFAIGARVEHPQDLINKAQYKDFYNHPRLGAADYRLIEHVSNGRTAYTFCMCPGG
                     SVIASASNDFEIVTNGMSEHARDKINANSAFLVNVTPEDFKSDNPLAGVHFQQKYEKL
                     AFELGGKNYNAPVQLVGDFINNKTSLNLGSVEPSYRPGYTLTNLSECLPSFVTETMRE
                     AFISLDKKLNGFAMYDAVLTGVETRSSSPIRIVRDENTLESVSIKNLYPCGEGAGYAG
                     GIVTAAVDGIKCAEKIIQKYTNNTKI"
     gene            34353..35981
                     /locus_tag="ACOIAF_001068"
     CDS             34353..35981
                     /locus_tag="ACOIAF_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HGZ2668089.1"
                     /translation="MNIIISLMGGLGLFLYGMNLMGEGLQKSAGTKLKKIIKLLTSNL
                     FMGVLVGTGVTAVIQSSSATTVMVVGFVNAGIMTLKQAIGVIMGANIGTTVTAQLVSF
                     DLTGMAPVALGVGIILYLFGNKPRIKNIAEILIGFGILFTGMDFMKMAVEPLRDYQGF
                     TDLLVTFGRYPLLGLLLGFGITAIIQSSSASMGMLVALAAEGLVPLSAALPILYGQNI
                     GTCVTSLLSSIGANKNARRAAMMHLIFNVLGTVIFLIFLNKPVVSMVTSWDPSNVARQ
                     IANTHTLFNIISVLILLPFTNLIIKLAIKLVPDRAGDIDEDETKTIKYIDDRMIETPS
                     IALANTIKEALRMGEKAKESLNASMEALVEHSTEKIDKTYRRERLINDLQKAILNYLL
                     KLSKAPLDDDSREVVDTLFNTVNDIERIGDHAKNIAELSQVAIDSNISFSEEGQSELD
                     VMYNRVVSAYTYALESMRTDNVDLACKVIKIEEQVDIMEKSCRANHMYRLNNNLCSIE
                     NGVIYLDVISNLERISDHAVNIAQQVIAKRLGND"
     gene            36246..37595
                     /locus_tag="ACOIAF_001069"
     CDS             36246..37595
                     /locus_tag="ACOIAF_001069"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HGZ2668090.1"
                     /translation="MGKINFDYSKATEFFCQNEIDVMQPYVDVAHDMLHNKTGLGNTF
                     LGWIDLPKNYDKEEFDRIKKSAEKIKSDSDVLLVIGIGGSYLGSRAAIDMVSHSFRNG
                     LKKEQRKAPEVYFVGHNISSTYIMDLLDIIEGKDISVNVISKSGTTTEPALAFRIFKD
                     YLEKKYGKEEARKRIYATTDASKGALRQLATEEGYETFVIPDDVGGRFSVLTAVGLLP
                     IAAAGLDIDAMMKGANDAREAFQNPDLKSNDSYRYAVARTILHRKGKDVELLVNYEPQ
                     LHYVSEWWKQLYGESEGKENKGLFPASVDFSTDLHSMGQYIQDGKRLLFETVLNVENC
                     KRNITISSEEVDLDGLNYLAGKTVDFVNHKAFEGTLLAHTDGKVPNLVINIPQLDEYN
                     FGYLVYFFEKACGISGYLLGVNPFDQPGVEAYKKNMFALLGKPGYEKEKEELEKRLK"
     gene            37751..38830
                     /locus_tag="ACOIAF_001070"
     CDS             37751..38830
                     /locus_tag="ACOIAF_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HGZ2668091.1"
                     /translation="MSRRKKGISLVILVAIISSILSSFLTIILVKDNLVSKSTGSSTP
                     IVVNDDGKSQNIYQAVAEKATPSVVGITTTSVDTSNMFAIPTETQGVGTGIIVDSNGY
                     ILTNSHVISDGQATSVNVLFNDGSTTSGKVVWFDQQLDLAIVKVDKTGLTPAEFADSD
                     KVKVGDISIAIGNPLGLDFQKTVTQGIISGLDRTIQTEKTNMTGLLQTDASINAGNSG
                     GPLLNQKGQVIGINTAKASQAEGLGFAIPINTAKSIVEEVIKNGKYEKVTLGIKGTDV
                     SNYEAATGTKLSTDKGVYVAEVISGSSAEKAGVKVGDIITKVGDTDITGMNDLNKKLY
                     TFSKGASTKITVNRGGKAVTINVNF"
     gene            39175..40083
                     /locus_tag="ACOIAF_001071"
     CDS             39175..40083
                     /locus_tag="ACOIAF_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019471 - 4-hydroxyproline metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-4-hydroxy-L-proline dehydratase activase"
                     /protein_id="HGZ2668092.1"
                     /translation="MNPLVINLQKCSIHDGPGIRSTVFFKGCPLECVWCHNPESQTYT
                     KQVLYNEERCSKCEACINICPHKAIYKGETKICLDQDKCEFCETCLDYCVNNAREIVG
                     QEYSVRDLVKEIEKDRIFYEESGGGVTLSGGEVMAQDMDFICGVINMCKSKGIHVAID
                     TCGYAKSENYERVAKCADLFLYDIKLIDEDKHIKFTGKSNDLILKNVKILSELGANIN
                     IRIPLIVGVNVDDENLEVKKMIEFLKPLNIQAVSLLPYHNIGKHKYDKIYKKYEGEEL
                     QRPSEEKLEEIKRLFEASNFNTKIGG"
     gene            40088..42457
                     /gene="hypD"
                     /locus_tag="ACOIAF_001072"
     CDS             40088..42457
                     /gene="hypD"
                     /locus_tag="ACOIAF_001072"
                     /EC_number="4.2.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019471 - 4-hydroxyproline metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-4-hydroxy-L-proline dehydratase"
                     /protein_id="HGZ2668093.1"
                     /translation="MARGTFERTKKLREESINAEPHISIERAVLMTEAYKKYEGSVEI
                     PVLRALSFKHYIENRTLSINDGELIVGEKGDSPNGAPTYPEICCHTMEDLEVMHNRDI
                     INFSVSEEARKIHKEEIIPFWKKRQTRDKIINAMTPEWLAAYEAGMFTEFMEQRAPGH
                     TVCGDTIYKKGFLDLKKDIEARLKELDFLNDLDAYNKKADLEAMAIACDAMVILGKRY
                     AEKARQMAEEETDEAKKQDLLLIAETCDVVPAHKPETYHQAIQMYWFVHIGVTTELNI
                     WDAFTPGRLDQHLNPFYERDVENGILDRDRAQELLECLWVKFNNQPAPPKVGITLKES
                     STYTDFANINTGGINPDGQDGVNEVSYIILDVMDEMKLIQPSSNVQISKKTPQKFLKR
                     ACEISRKGWGQPAFYNTEAIVQELMEAGKTIEDARLGGTSGCVETGCFGKEAYVLTGY
                     MNIPKILELTLNNGYDPISKKQIGIETGDPRNFKSYEELFEAFKKQLHYMIDIKIEGN
                     AVIENICAKHMPCPLMSTIVDDCIEKGKDYQRGGARYNTRYIQGVGIGTITDSLTAIK
                     YNVFDKKKFDMDTLLKALDANFEGYEAILNLVSNKTPKYGNDDDYADEIMQEIFNAYY
                     NEVTGRPTVCGGEYRVDMLPTTCHIYFGEIMGASPNGRLCAKPVSEGISPEKGGDTNG
                     PTAVIKSCAKMDHIKTGGTLLNQRFAPSVVQGEKGLDNMANLVRAYFNMDGHHIQFNV
                     FDKNVLLEAQKNPQDYKDLIVRVAGYSDHFNNLSRTLQDEIIGRTEQTF"
     gene            42782..43585
                     /gene="proC"
                     /locus_tag="ACOIAF_001073"
     CDS             42782..43585
                     /gene="proC"
                     /locus_tag="ACOIAF_001073"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HGZ2668094.1"
                     /translation="MKTLGFIGSGNMGSAMIGGIVQSGLVKAEDITVSDLSQVALDKV
                     KEEFGVNTTTDSKVAVTNSDVVIVALKPNICEKALTPLKELIDANKIIVSIAAGKTIE
                     SLESFIGSDKKIVRVMPNTPALVGEGMSALCKNSNVTDEELNMIKALFESFGEAEIVS
                     EYLMDTVTGISGSSPAYVFMFIEAMADAAVLAGMPRQQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPAGTTIEAVKVLEEEGFRAAVIKSIVACIDKSKDMSK"
BASE COUNT        17980 a         4690 c         7956 g        13118 t
ORIGIN      
        1 atatactgac attacaagca tttttatata tggcaatatc tccaatacca acacctggga
       61 atgtaggagc taatgaatta gcctttttca ctatattcaa atcagtattc cctcaacctt
      121 tgatgggata tgcagtattt ttatatggtg gatttatgta ctatttgatt ctgattggaa
      181 gtggaatatt tactgtaatt actcactata gaatgaaaaa tagaattggc agaaatattg
      241 ttttaagtga aaactaaata agtttaaaga atatatattg agtatattaa aaaatatgtc
      301 ataagaacta gaatatttta attaaatggt tgaaatataa agattattat gacatatttt
      361 ttttgaaatt taagaggaat tgtgagaaaa ttgttgaatt taatagatag tattgcaaaa
      421 tatttatgat tttgcatatt ataaaaacac tcattatctt taagataaat tttacttatc
      481 aggggataaa tctaaggggg gaattgtatg gagaaaaaaa ttttaacagt ttgtccatac
      541 tgtggagctg gatgccaatt atatctagta gtaaaagata ataagatatt aagagctgaa
      601 ccggctaatg gtagaacaaa tcaagaaagt ttgtgtttaa aaggtcgtta tggatgggat
      661 tttctaaatg accctaaaat tctaacacct aggctaaaaa agccaatgat aagaaaacaa
      721 ggtgtactag aagaggttga atgggatgag gctataaact atacagcttc aagactaaat
      781 gaaattaaag aaaaatatgg acctgattct attatgggaa caggttctgc aagaggacct
      841 ggtaatgaag caaactatat aatgcaaaaa tttatgagag ctacaatagg aactaataat
      901 gttgaccatt gtgctcgtgt ctgacatgca ccatctgtag ccggtctggc ttactcatta
      961 ggtagtggtg caatgtctaa ttctatacca gagatagaaa atgcagatgt tttatttata
     1021 tttggatata atggagcaga ttcacatcca atagtggcaa atagaatagt aaaagctaaa
     1081 aaaaatggtg ctaaattaat tgttactgac cctagagtaa ctgaatctgc aagaattgct
     1141 gatatacatc tacctataaa aggtggaaca aacatggttt tagtaaatgc ttttggaaat
     1201 gtattaatag aagaaggtct ttacaataaa gaatttgttc aaaatcatac acaaggattt
     1261 gatgagtata aagaaattgt taaaccatat acagctaaat atgctgaaaa gattacagga
     1321 atcccagaag agcttataag aaaagctatg agagaatatg caaaaggtaa aaaagctatg
     1381 atactttatg gaatgggtgt ctgtcaattt ggtcaagctg ttgatgtagt taagggactt
     1441 gcatcaattg ctctacttac aggtaatttt ggtagagaaa gcgttggaat aggacctgta
     1501 cgtggacaaa ataatgttca aggagcttgt gatatgggag ctcttcctaa tgtatatcct
     1561 ggctatcaaa atgtaactga tgataagata agagaaaagt ttgaaaaagc ttggggggta
     1621 aaattatctc ctaataatgg ttatagttta actcaagttc cagacttagt actaaaagag
     1681 aaaaagctta aagcttatta tatatttgga gaagacccag tgcaaagtga cccagatgca
     1741 tcagaagtta gagaagcatt agatgaattg gaatttgtaa ttgttcaaga tatatttatg
     1801 aataagacag cacttcacgc tgatgttata cttccagcta cttcttgggg agaacatgag
     1861 ggagtatata catgtgcaga tagaggattc caattgatga gaaaagctat agagccacaa
     1921 ggagatgtaa aaccagactg gcaaattata agcgaaattt ctacagcaat gggatatcct
     1981 atgaattata aaaatactaa ggaaatatgg gacgaactta gacaattatg tccaagtttc
     2041 ttaggtgcta cttatgaaaa gatagaaact caaggatgcg ttcaatggcc ttgtaagagt
     2101 gaaagtatgg aagataaagg aactatgtat ctgtatgaag gtcaaaagtt ttctactcct
     2161 aatggtaaag gtaatttgtt cgctgctgaa tggagacccc ctatggaagt tgaagatgat
     2221 gaatacccat ttagtttatg tactgtaaga gaagttggac actattcagt aagaacaatg
     2281 actggtaatt gtagaacact tagttcatta gaagatgaac caggacgtgt tcaaataaat
     2341 tctaatgatg ctgaaaaatt aggaattgaa gatgatgaat tagttagaat tagttcaaga
     2401 agaggaagtg taataacaag agcaacagtt actgatagag ttaaggaagg tgcaacatat
     2461 atgacttatc agtggtgggt tggagcttgc aatgaactta caatagcaaa tttagaccct
     2521 atatctaaaa caccagagta taaatattgt gctgttaaac ttgaaaaact tgaagaccaa
     2581 gaattagcag aaaaatgtgt aagagaagag tatcaaagtt tgaaagacaa aatgacagct
     2641 acaaatatct aatgatttat tagatttaag atttataaaa ggcagaaagg ctaatatttt
     2701 atgaacaata tatactcaaa aactaaatta caagaaaata gtaaggataa agtttttgaa
     2761 aatagttata gtctagtgga ctacaaatat ttaaattctt ttaatgtaga agtaagcaaa
     2821 ataaatgaac atgaagtatc aatagataat gaagaaataa ttgcagaata tccacttagt
     2881 tttattttaa atgataaata tataaataca tttttatgta ccccatataa tttagaggaa
     2941 cttatagtag gattcttgaa aacaaaagga tacatagaaa acaaaaaaag cttattaaaa
     3001 tttgagataa atgagagtga acgttttgca aaagtaacta ttcaagagaa acaaaaaaat
     3061 caagatgagc aaattatatt tttaaattca ctagactata taacatgtgc tcctgtaaag
     3121 agcaatataa aaataaatta tgatacgatt tacagtataa tggataaaaa tttaaattca
     3181 tcagaattat ttaaaaatac tggtggggta catagcgtat ctgtatttaa ttttgataaa
     3241 aaaatagtca cttgtgaaga tgtagcaagg cataatgcaa tggataaggc tattggattt
     3301 tgtgttttaa atgacataac attaaaagat aaaattgtgg ctgtaagtgg aagaatatca
     3361 ttggaaatga tattaaaagc agctatgact caaattccta ttgtagtatc aaaatcagca
     3421 ccgactaatt tatcaataga gctttctaaa aaattaaata taacgcttat tggatttgta
     3481 agaggaagac ggatgaatat ttatgcaaat ccacagagag ttatcaaaaa gtgagaatga
     3541 atatatgtgt actaaaaaag aagagcaaag atattcaaag tgtgaaatct gtggagtatt
     3601 aattgatgat actgataagg tatataataa cacatacaat ttgtgcaaaa aatgtaaacg
     3661 aaatataagt actgagaaaa tagtgaaata tataaatata tatgatgaaa ataactagag
     3721 ggttggaaat atacaaattg aaaggtggga taatatgaaa aataaaatag gctcttttgt
     3781 aatagcagac ccaaacaaat gtacaggttg tagagcttgt gaagtagctt gttttacaat
     3841 gcataatcag cataataatg tgggttatac agttggaaca gttgatatac ctgttatacc
     3901 tagactttac ttagtaaaag gtgacaattt ctgtatgcca atacagtgta gacattgtga
     3961 agatgctcct tgtttaaact catgtccaca aaaagctata gttaaagaaa acaatattat
     4021 gtctgtaaat gaagaaaagt gtataggatg taaaacttgt ctgctagcat gtccttttgg
     4081 agctatagac ttacttccac aatatgaaga tggtaaagaa gtagaacaag taattttaga
     4141 tgaaaataag aagatagctt ataaatgtga cttatgtaaa gacaatgaaa aaatagcttg
     4201 tataagtgca tgtcctcagg aagcattaaa acttgttact ccaatagatg acaaaaaagc
     4261 taagaataga caagctgcac ttagtttatt gttaacaaat aaataaagga tacaggggta
     4321 ggtgacgttt atgataataa atatagataa ggatttatgt actggatgta gagaatgttc
     4381 aaaggtctgt ccagtaaatg ctattgaagg tgaagaggga aagccacaag agataaattt
     4441 agatagatgt gttatgtgcg gtcaatgtgt tcaaacttgt aaatcttatg cctcagttat
     4501 agatgaagga tttgagtttt tacaagagaa aaaacaagaa agagagatac cagagtctat
     4561 aaatgaacct atatttgcag catacaatgt atgtgatata gataaggtga agaaagcttt
     4621 aagtgaccca aatgtattca ctatggtcca atgtgcacca gcagttagag ttgctttagg
     4681 agaagatttc ggatattatt taggatactt aggtgctggg aaaatggcag cagcacttag
     4741 agctttaaat tttgatagag tctacaatac taattttgca gcagatttaa ctataatgga
     4801 agaaggtagt gaattaatta aaagagtgac agaaggtgga actcttccaa tgtttacatc
     4861 ttgttgtcca gcttgggtaa aattcatgga aaaaaattat cctaaattaa ctaatcattt
     4921 atcttcttgt aaatcacctc agcagatggg tggtgcaata tttaaaactt atggtgcaga
     4981 gataaataat gtagatgcat caaagatatt tagtgtttct ataatgccat gtacatgtaa
     5041 aaaatatgaa tgtgatagag aagaaatgga ttctagtgga tatagagatg tagatgtagt
     5101 attaactact agagaactag cttatttaat aaaagacatg ggaattgatt ttaaaaattt
     5161 aagagaagaa aaatttgata gtcctttagg tagttatagt ggagctggaa ctatctttgg
     5221 tgttactggt ggggttatgg aagctgctat aagaacagga tatgagctta taactggaga
     5281 aagtattcct gatgtagaag taaaacaagt aagaggagaa aatggtttta gaaagtcaac
     5341 tattaaaata ggagacttag acttaagagt tggagtagta agtggattaa aaaatgtaat
     5401 accagtgctt gaagacttgg aaaaaggaaa attagatgta gattttattg aagttatgac
     5461 ttgtcctgtt gggtgtgtaa gtggtggagg tcaaccaaaa attttactag aagaatataa
     5521 ggaattggct tatgaaaata gaacaaaagc aacatacgtg catgatgaac atttagcaat
     5581 tagaaaatct catgagaatc ctgatattaa aaaactgtat gatgaatatc tggtagaacc
     5641 attaggagaa aaatctcatc atctattaca cactacatat tgtataggaa aggaagtggc
     5701 taagtgatga actattttgt tatagcagac cctgataaat gcataggatg tagaacttgt
     5761 atgataggat gtgtagttgc acattcagat gaagatattt tttaccaaaa tccagatgaa
     5821 ataaatttta atccaaaact ttctgtcata aagacaaaag aagtaagtgc tccaatacag
     5881 tgtagacatt gtgaagatgc tccttgtgca aaggcatgtc caaatggtgg tatagttagg
     5941 gttggtaaca caataaaaat caatgaagaa aattgtatag gatgtaagac ttgtatgtta
     6001 gcatgtccta taggtgcaat agacatagtt acattaaaag atgtagatga aggaaagctt
     6061 tgctttagag aaagaatgac agcaaataag tgtgattttt gtatagaatc gccagaaggg
     6121 cctgcatgtg taaatgtttg tccaactaag gcatttacta tagttaagga tgaggatata
     6181 gatagtaaag ttaaaaataa gagaaaatta gctatattgt aatataaaaa agatggagtt
     6241 ttatttatga ttaaaagctc catctttttt attataatat ttatatatct aaaaatcaat
     6301 tattgcatta gtttatctaa tctttcttta ttcaattttt tcattcctat agaagttata
     6361 ataagtaaag tataggcaat tataactgat aaaattataa tagaccaaga aaatctgtat
     6421 ccaaaattat caactaacaa tccaaataat gttgaaccta atgcaaatcc cactgcaaaa
     6481 attaattgta cgattccata tatgctacca aactcctttt gaccaaagaa agagccagtt
     6541 aaataagcag gaccaatcat ataagcaaat acagaaagac ctttcaaaat ggcaaataca
     6601 aatgcaaaag tagaagattt atctaccaat agtaatgata aggttgctat aagaaccaat
     6661 acaaaagata taataagtgg ctttgtttga ccgaatttat caaacaataa tcctccaaat
     6721 aaattaccaa tgagagcaaa taaagcaaat gtggagccta ctgttcctat tagaatagga
     6781 tttagataat ttctaagata agttggatat tgtacagata atgatgaaac atatatacca
     6841 acaaatataa atccaatagt cataatccaa aaatatttaa tttttttagc ttcttttaat
     6901 gtataacccc atactgaagg agaaattgta tcagatgctt tctctttttt acctttagat
     6961 tttacctttt cattaatatc tttaggcatc ctgagtataa ataatattac aggtatacca
     7021 actattaatg ctataattcc aaatattaga taagaacgac tgtatccttg agttgctaat
     7081 aagcgaactg caagtggttg tagaaatata ctaccaatag aaccaccagc aaaagcaaag
     7141 cccaaagctt ttcctttgct ttcttcatca aaccaagcac ttattaaaac aggtattcct
     7201 atagcagata taattgtagc gccaatttga acaatacatg atacagtgta aaattgccat
     7261 agttcagtac atatagaaaa agtagcaaaa gcaatactag aaattataca acctaataaa
     7321 tatagtatct taatattgac tttagaaaat aatacaccta taaaaggtga tgcaattgct
     7381 gaggcaatag taccaatagt gaatattaga gaaaaagaag ctaaactaaa gccttctcct
     7441 tctacaacat aacttataaa ttgaggctgt atacttgcta caatactaaa tggaacagct
     7501 tgtattaaca tacaaccaag tactataacc caagcatttg aaaagttttt aatgctaaat
     7561 ttattgttta gtaatgtatt catggtatct ctcctttagg ttatgatttg tagatattaa
     7621 tctaattatt ctaattttat atattagttt tggatagcct aactatttaa atataatttg
     7681 ggtattaata taaaaattaa ctataaaaat tgattatttt aaatgtatgg ggtaaattaa
     7741 agtatataaa taaatgaaag gatggataaa atgtcatttg aaaaaataaa taataataac
     7801 ctagataaaa tacacaacag gaagtgtatt atgcttgtaa actttaataa aaaagaatta
     7861 tcattaatta aaaacgtaag taactttgct ggaataagag accatatcat cttaagtgat
     7921 aaaaatggag agaatataat aagagacatt ttagatggaa atatatcaaa tgaatgtgaa
     7981 aatgggatta agagtaagtc tgtaatattt aataacactc caagcacaca agtaaatgca
     8041 tttttagaag ggctaaaaaa agtgagaata aatagacctt ttacagctat ggtaacagag
     8101 acatctattg attggacatt gaataattta atagaaaatt tagttcaaga acgtaatgct
     8161 atgtctttag gaaaaacaat tgaacataag taaatttaag atatcttata gtaattttaa
     8221 ctaatgtaca ttgagtatca tattatattt tagaatataa aattttaaat gtgggtagat
     8281 ggaggagatt tgagatgaaa gttttattta caataaatta tggaaaagaa aaatttgata
     8341 aaataagaga attaggctat aatgtcatac attactctga agatagtatt gaaaataatg
     8401 aggatgtaaa tacagcagat gtactagtta catacaatcc atttgagaga ttagatattt
     8461 ctaagatgaa aaatttacaa tatatacaga caactagtgt aggtatagac caacttccaa
     8521 aagaagaggt attgaagaga aatattaaga tagctaataa tagaggcgga tatagtgtac
     8581 ctattggtga aagtattgta ttgtatattt tagaaatata taaaaatagt gcaaaatttt
     8641 ttaaacaaca acaaaataaa gaatggaaga tggatttttc tgtatcagaa ttgtatggac
     8701 aaagagttgg atttttagga acaggtacta tatcttcaga agcagcaaaa agattaaaag
     8761 cttttggtgt agaaatatgg ggtgtcaata ctgatggaag tagtaaaaaa tactttgata
     8821 agtgttttgc tactgaggat atgaatattg tatttgaaga atgtgatata gtagttgtag
     8881 caatgccttc tacaaaaagt acagatggaa tagtaaacaa agatatgttt aaacttatga
     8941 aagatggttc tgtatttata aatgtaggta gaggaaatac agttaatcag aaagatttag
     9001 aagaatgtgt agagaaattt agaggggtag cattagacgt atttgaaagt gaacctttaa
     9061 acaaggacaa taagttgtgg gagtgcgaaa atgtcatagt aactcctcat aattcttggg
     9121 tgtcagataa aaacaaagag agaacattta atatggttta taataatttg aaacactata
     9181 taatggagaa taaacctgta gataatgttg tagacatatc aagaggctat taaaaataaa
     9241 aaattttgat aaaaataaaa actcttatta gtcgtattaa agatactaat aagagttttt
     9301 tttgtaataa atttaaagcc aaaaaagtga tatactaaaa aatatataaa taggagataa
     9361 ttaattatag ggaaatttca aaataattta attttaaaaa tatgaagagg tgcaaaaagt
     9421 gagcgtaaaa gatgaaatta ataaacttat agagacaata aactaccata atgaaaggta
     9481 ttataatcaa gatagcccag aaatatcaga ttatgaatac gataagttaa tgaaggagtt
     9541 aatttcttta gaagaagaac atccagaact aaagagaatt gattctccaa caaatagggt
     9601 aggaggtaag ccattagata agttcaatca aattgtacat aaaactccta tgttgagttt
     9661 atcaaatgct ttttcagagc aagatttgag agattttgat aagagagttc aagaatatgt
     9721 gggagaaaat gttgaatatg ttgttgaatt taaaatagat ggtctttctg taggtctaac
     9781 ttataacaat ggagagtttg aaaaaggagc aactagagga gatggagttg taggagaaga
     9841 tatttctcaa aacttaatga cagtaaaaag catacctctt aagataaatg ataaaaaaga
     9901 actaatagtt agaggagaag tatatatatc aaaagataat tttagaaaag taaatgaaca
     9961 acaagaagaa caagaacagc cattatttgc taatccaagg aatcttgcag ctggttcatt
    10021 gagacaatta gacccaaaac taacagcaaa gagaccatta gatatatttg tatttaatat
    10081 ggaaaatata gaagaatacg atttagaaag tcacagtgaa tcactagagt acttagaaaa
    10141 actaggattt tcagtaagtc agagttacaa agtttgtaaa agtatagatg aagttattga
    10201 gcatattgaa tactggacag ataacagggg taatttagac tacgaaatag atggtatggt
    10261 aataaaagta aacaatataa aacaaagaga cgaaatggga tatacagcca aaagtcctag
    10321 atgggcaatt gcatataaat tcccagcaga aaagaagaaa actaaaatag tagatataat
    10381 agtagaagtt gggagaactg gtacaataac accttcagca attcttgaac cagtcagact
    10441 tgcaggtact actgtaagta gagctacact tcacaatgaa gattatataa gagaaaaaga
    10501 tataaaaata aatgatacag tattagttca aaaagcaggg gacataatac ctcaagtttt
    10561 agaagttata aaagaagaac gtactggaga tgaaatagat tttaaaatgc cagaaaagtg
    10621 tccagtttgt tcagagccaa caataagatt agaaggagaa gctgctgtaa aatgtatcaa
    10681 tatgtcttgc ccagcccaaa taagaagggg aattatacac tttgtgtcaa gggatgcaat
    10741 gaatatagat ggattgggag aatctataat tacactgcta ttaaatgaaa aaattattca
    10801 agatgtatct gatttatatt atataaaaaa ggaagatgta gtcgacctag agagaatggg
    10861 cgaaaaatct gctgacaatt taataaattc aattgaaaaa tcaaaagaaa atgatttatg
    10921 gagacttata aatggattag gtattaaatt tataggagtt aaagcagcta agatacttgc
    10981 atataacttt aaggatttag atgaagtaat ttctgcaact agtgagcaac tagaagaatt
    11041 agaggaattt ggaagtataa tggcaaacag cgttgtcgag ttttttaagg aagacaaaaa
    11101 tatgaacgtt ataaacaagt taaaaaatgt aggagtaaat acaaaatcat tggataatac
    11161 aggtgaaaaa gtagaaaaaa tatttgaagg gatgaaaatc gtattgactg gtacacttcc
    11221 tacattaaaa agaaatgatg caaaagaaat gatagaaaaa agaggtggaa aagctacatc
    11281 tagtgttagt aaatcgacaa catttgtttt ggcaggtgaa gaagctggtt ctaaacttac
    11341 taaagcaaat gatttaggaa ttaaagttat agatgaagag agatttttag aattattaaa
    11401 gttatcatcg aaagatgaag taaaagctgt attagagagt taaaaagtcg cttaatttag
    11461 tagaaaacta ctataattaa tgatagaata gttcagtaag ctaataagat tacgaaaaat
    11521 gacaataatt aatatttgac aaaattaaaa attataaaaa taaatttata aaaaataaaa
    11581 aatatgagaa aggatataaa aaatgaccag aagttatgat agaagtaatg aacagataag
    11641 acctgtaaag ataactagag attatacaaa gtatgcagaa ggttctgttc ttatagaaat
    11701 gggagagaca aaggttatat gcactgcatc agtggaagaa aaagttcctc cttttttaag
    11761 aaatagtggt actggatgga taaatgctga atattctatg ctaccaagag caacacaaca
    11821 aagaaagata agagatgcat ctaggggaaa aattgatgga agaacacaag aaatacagag
    11881 gttaatagga agagcaataa ggtctgttgt agacttgaat aaattaggag aaagaactat
    11941 ttgggtagat tgtgatgtga ttcaggcaga tggtggaact agaactgcat ctataacagg
    12001 ggcatttgta gcagttgcag aagcaatata taaattatat aaagcaaaga caataaaaga
    12061 gatgccaata acaaattttg tgtctgcaat tagtgttggt attgtaaatg gaatacacat
    12121 gctagattta tgttatgaag aagattctaa agcacaggta gatatgaata taataatgac
    12181 tgataagtgt gagtttgtag aagtacaggg gacaggagaa gagaaacctt tttcgagagc
    12241 agatttaaat ttattgttag agttaggtga aaaagggaat aaagaattaa taaggcttca
    12301 aagagaagct ttaggtgaaa tatcagatga gatattgggt atggattatg gtaatgaagt
    12361 agtgattgct actaataatg ttcataagct agaagaaata ggtgaaatct taaaagattt
    12421 tgaatataaa gtgtactctt taaaagatgt tgatttagca ggtatagaga tagtagaaga
    12481 tggaaaaaca tttgagcata atgcgttgat aaaagctaga acaatagcaa aaaagactaa
    12541 acttatagca atatcagatg attctggctt agaagtagat gctttaggca aaaaacctgg
    12601 tgtttattca gctagatatg caggagaaca tgcaacagat gaagaaaata gaaaaaaact
    12661 gttaaaagct atgcaaaatg taccaatgag caaaagaaat gcaagattcg tctctgcaat
    12721 agcagtagtt tttcctgatg gaaaagaatt tgtagtaaga ggaatttgtg aaggaatgat
    12781 aggatttgaa gaaaaaggta aaaatggttt tggatatgat agcttattta tagtaaaagg
    12841 atatgataag acttttggtg aaatacctag tgttataaaa aattcaatca gtcatagagc
    12901 aaacgcacta aaacttatga aacaagagtt tataaaaagg gtagtgaagt aatgaaaata
    12961 ggcatagtaa gcgacacaca tatgatgatt aaaaatatgg aaaaaacaat accatatttg
    13021 aaagaatgtg acctaataat tcatgcagga gataacttta ctgattcaag atatattcat
    13081 agcatgacta atgtaggtat tattgcagtt aaaggtaatt gtgactttga tgctgttgag
    13141 gaagaagttg tctttgaagt ggcaaataaa accatattct tatgccatgg agataagtat
    13201 ggtgtaaaat atggaactaa tatgttagaa aaaaaggcaa ctgaagttga tgctgatata
    13261 gttatatttg gccataccca taccccattt agagaaatta aagatggtgt actttacata
    13321 aatcctggaa gcacttcact tccaagagga gtatcgtata aaagttttgt cattatggat
    13381 atagaagaag atgacataaa aattgaagaa atacgcatat aaaggtgtat ttatctaaaa
    13441 aaatggcaaa aaacgctcaa aaaattaaat tttatcaaat aagtacaacg atattaatgg
    13501 aacacttgta aataaaaaaa cttatgtgat ataattaagt ggttgaatta taaatagaat
    13561 gaattataat accgttatcg ggtattacat aaatatcact aggaggatta gaaggatgaa
    13621 agcagaacta attaaaaaag agggtaataa agttacttta aaaataacag tagataacaa
    13681 taaatttgaa gcagcagtaa ataaggctta taataaaagc agaaataaat acaatatacc
    13741 tggatttaga aaaggtaaag ctccaagaat tgttatagag actcaatacg gaaaaggtat
    13801 attctacaat gatgctatag aaatattatt cccagaagtt tatccagaag ctatcaagga
    13861 attagacata gacccaatag acaatccaga tttagatata gaagaaataa gtaaagacaa
    13921 tgggttggtt atggttctta atgtagaagt taaaccagag tttgaattag gaaactacaa
    13981 gggtatagaa atagctaagg ttgagaatac tgtaagtgac gaaaatgttg atgctaaact
    14041 acaagaaatg gttgaaaaaa atgccagatt agtaagtgta gaagacaaag ctttagaaga
    14101 tggagataca gctataatag acttcgaagg ttttgaaaat ggtgtagctt ttgatggtgg
    14161 taaaggtgaa aattataact tagttatagg ttcaaacaca tttataccag gatttgaaga
    14221 acaattatta ggtaaaaaag ctggtgaaga agtagaagtt aatgttactt tcccagaaga
    14281 atatcactca caagatttag ctggaaaacc agttgtattt aatgtaaaaa taaatgatgt
    14341 aaaagtaaaa gaattatctg cacttgatga tgaatttgct aaagatacta gtgaatttga
    14401 ttctttagat gaattaaaag cagatgtaag agctaagtta gaagaagaag ctaaaaatag
    14461 agcagatgca gaaactagaa attcagtagt agaaaaagtt gctgaaaata cagaaataga
    14521 aataccagaa gttatgatac aacatcaaat agataatatg ttaaatgaac ttaactacca
    14581 attacaatat caaggatttg gattacaaca attactagag atgactggta gaactatgga
    14641 agaattaaga gaagagagaa aagaagatgc taaaaaatta gttaagtctt ctttagtatt
    14701 agaagcaata actaaagctg aaggaattga agcaacagaa gaagaattta aggctgagtt
    14761 agaaaaaatg gcttctgctt acaatatgga agtagaaaaa atagaagctt ctttaagaga
    14821 tgctgacaaa gaagatataa aaggtcaaat aaaaataaga aaaacaatag atttattagt
    14881 tgataatgca acaatagctt aataaaataa acgctaacta atatagttga cgttatgaaa
    14941 tgtttagtat ataatgatgt gcaaatataa tttagttaaa tgcgtataat aatttacagt
    15001 agcttaaaaa aagctactgt aaattatata gaataaaggg ggtataaaac catggcatta
    15061 gtacctgtag ttgttgaaca aacaggaaga ggagaaagat cttatgatat tttttctaga
    15121 ctattaaaag acagaataat cttcttagga gatcaagtta acgatgccac agcagggctt
    15181 atagtagcac agctattatt tttagaagct gaagacccag acaaagatat acatttatat
    15241 ataaattctc caggaggaag tataacttct ggaatggcta tatatgatac tatgcaatac
    15301 atcaaaccag atgtaagcac tatatgtata ggtatggctg cttctatggg agcattcttg
    15361 ttggcagcag gagcaaaagg aaaaagacta gcactaccaa atagtgaaat aatgatacat
    15421 caaccattag gtggagctca aggtcaagca acagatatag aaattcatgc aaagagaatt
    15481 ttaaaaataa aagagactct aaatgagata ttctctgaaa gaactggtca gccattagaa
    15541 aaaattaaaa tggatactga acgtgataac ttcatgagtg cactagaagc caaagaatat
    15601 ggtttaatag atgaagtatt tacaaagaga ccgtagatag agagaggtgt taatatgtct
    15661 aaatatgaag aaaaacgaca attaaagtgt tcattctgcg gtaaaaatca agatcaagta
    15721 agaagattaa ttgcaggtcc aaatgtatat atttgtgacg agtgtgttga actttgtgac
    15781 gaaattattc aggaagaaat agaagatact atagatgaag atactacatc tttaccaaaa
    15841 ccaaaggaaa tgatggagat tctaaatgat tatgtaatag gtcaagaaaa agctaaaaaa
    15901 gctttatcag ttgcggttta taatcattat aagagaatat acagtaaaaa atctagtagt
    15961 aaggatatag aaattcaaaa gagtaatata cttttattag gacctacagg ttctggaaag
    16021 acattacttg ctcaaactct agcaagaact ctaaatgtac cttttgcaat ggcagatgct
    16081 acatcattga ctgaagctgg atatgttgga gaagacgttg aaaatatact tttaaaactt
    16141 attcaagctg ctgattttga tatagaaaaa gctgaaagag gtataatata tatagatgag
    16201 atagataaga ttgcaagaaa atctgaaaac ccatctataa ctagagatgt tagtggtgaa
    16261 ggtgttcaac aggcacttct aaaaatatta gaaggtactg ttgcaaatgt tccaccacaa
    16321 ggaggaagaa aacatcctca tcaagaattt ttaaagatag atactactaa tgtattattt
    16381 atattaggtg gagcttttga tggattagag aagataattc aaaaacgtgg tggagataaa
    16441 acactaggtt ttggagctaa aatagaaagt aaaaaagaat tagatttagg gaagctatat
    16501 gaaaaagtac ttccagagga tttattaaaa tatggtataa taccagagtt tataggaaga
    16561 ataccagttt tagctacttt agagttgttg gatgaagatg cattaatgca gatattacag
    16621 gaacctaaaa atgctctagt aaaacagtat aaaaagttat tggagttaga tgatgttgag
    16681 ctagaatttg aagaaggtgc tcttcgtgca atcgctaaaa aggccataga aagaaataca
    16741 ggagctagag gacttagaag tatagttgaa agcgtaatga tggaaactat gtttgaagta
    16801 ccatctagag ataatataaa gaaggttata gtaacagaga aatctgtgaa cgaagactca
    16861 gtaaatccta tcatagtact taaggatcaa gaggaaagtg cataagatga tgaaaaggag
    16921 ctttaaaagc tccttttttt atgttaatat gctaaatata aaataagtat cattattata
    16981 aaaatgtgag taatatacct agaataccaa agacgaatac aattgcaatg gaaccaattt
    17041 ttcctttata taaaagataa aatgatgcta taaatgcaat tattccatat aaatcccaat
    17101 taacttcttt ggcagatgaa aaagctgctg ataatatgaa tcctagagta attggtctaa
    17161 gaactgtcaa taagttttct acctttttat tatttttaaa tttagaaaat aactttgata
    17221 taatagttag acctattaat gcaaaaattg taacaccaat agttgcagct agagaaccaa
    17281 agaagccagc agtcttaaat cctacaaatg tagcactatt tattgctaca ggacctggtg
    17341 tagattgtga tatagcaagt aaatccaaaa actcttgatt agttataaag tgatatttag
    17401 atataaattc ttgctggata aatggtagca tggcataacc gccaccaaaa ctaaatgccc
    17461 ctattttaag gaatattaag aagagtagta ttatttctga catagactat atacacctcc
    17521 tccaattatt aaataaatag ggcttatatt gaatacacca acagataaaa ctgctataat
    17581 taatagtagt atgttagttt tagactttgg tagtttcttg aacatgctcg taaaagaata
    17641 caatataaga gctattacaa caggcgcaac tcctttaaaa aatccatcta aaattttaga
    17701 tgatgaattt ttaaagtata gatacgataa gactaagact atacaaaatg atggtaaagc
    17761 agagcctaag gtacatataa gaactcctgg tataccataa agtttatatc ctaataatac
    17821 agatatatta actgccagta ctccaggata actttgagct atggataaat aatcaataaa
    17881 ttcttcctgc tctaaaagtt tttgtttgtt tacaagttca tcttgtataa gaggaaccat
    17941 agcgtatcca ccaccaaatg taaacgcacc aatttttaaa aaggttaaaa acaatttcaa
    18001 cataatagaa acctccaaaa gataaataac atctaagtat actaatatta atattatatc
    18061 ataataaaaa gataatatag tatgatagta ttctaagaat ttatcattaa tgttgaaaat
    18121 taacttgttc aaatatataa tattaaaagc aaagcaatta gactagaaaa ggagtaatgt
    18181 tgatggaaca aaattatact aaaatagatc atgaattacc attaattcct ctaagaggat
    18241 tagctatatt tccatatatg atattaaatt ttgatatagg tagagagata tctcttaaag
    18301 ctttagacca agctatgatg gatgaagaac ttatattttt aacttctcag aaagaagcag
    18361 aggtagatga gccaggagaa gaggattttt atcatgtggg aacaatctgt aaagttaagc
    18421 aaatgataaa attaccagga gatacagttc gtgttttggt tgaaggtgta tcaaggggta
    18481 gagtaaagaa aatagaacaa gaagatgggt atttcagagc agtaatagaa gaaatagtat
    18541 ttgatagtga taatttggat agtgaaacag aagttgaaat tgaagcattt gtaagaaatg
    18601 tatttgatgc ctttgaagag tatataaata ttggaaatag ggtatcacca gaaatattaa
    18661 tatctttagc tgatatagaa gatgtagata gatttataga tacgatagct gcaaacatat
    18721 atctaaaatc aagccaaaaa caagaaatat tagaagaatt tgatattaga aaaagacttg
    18781 aacttattta ttctatatta ttagaggaaa tagatatatt aaaaatagaa aagaaaataa
    18841 ctttaagagt aaaaaaacag atgaataagg ttcaaaaaga atattatctt agagaacaat
    18901 taaaagctat acagaaagaa ttaggtgaag aagaagatat aaactctgaa gcagatgagt
    18961 atagagaaaa gcttaaaaaa ataaaagcac ctaagacaac taaagagaaa atagaaaaag
    19021 agatagataa attttcaaaa atatcttcta tgtctccaga tgtatctgta agtagaaact
    19081 atttagatac tatattctca ttgccatgga ataaagagac aaaagataaa ttagatataa
    19141 ctaaggcaaa agatatatta gatgaagacc attatggatt agaaaaagtt aaggagagaa
    19201 tactagagta tctagctata agaactcttg ctaagtcact taaagggcct ataatatgtc
    19261 tagttggtcc tccaggaaca ggaaagacat ctatagtaaa atcaatcgcc agagcattaa
    19321 atagaaaatt tgtaagaata tctttgggtg gagttagaga tgaagcagaa ataagaggtc
    19381 atagaagaac ttatgtaggt tcaattccag gaagaataat taatggggta aaagaagccc
    19441 aaacaaaaaa tcctgtattc ttatttgatg agatagataa gatggcagca gattataaag
    19501 gtgatccagc ttcagcaatg ctagaagtat tagacccaga gcaaaataag gattttgtgg
    19561 accattactt agaaatacct tttgatttat caaaaatact atttgttaca acagcaaata
    19621 gtctaggtaa tataccaaga ccattattag atagaatgga agtaatagaa gtatctgggt
    19681 atatagaaga agaaaaatta aatatagcaa aaaaatacct acttccaaaa caaattaaag
    19741 aacatgcttt aaaagaaagc tttataaaaa tagatgatga aactttaaga agtataatca
    19801 accattatac aagagaagct ggagtaagaa cactagaaag aactattggt aagatttgta
    19861 gaaaggttgc caaaaaatac gttgaagacc caactcttga agaggttgtt ataaataagt
    19921 ctgacctaga gacatatctt ggaaaagaca tgtttaagta tcaacttgca gaagttaacc
    19981 ctcaaatagg attagtaaat ggtcttgcgt ggacagaagt tggtggagtt acacttgaag
    20041 tagaagtaaa cgtacttaaa ggtaaaggcg aaattgtatt aactggaaaa ttaggagatg
    20101 ttatgaagga atctgcgaag acaggtatct cttatataag gtcaatagtg gacaagtttg
    20161 atatagaccc tgaattttat aagacgaatg atattcatat acacattcca gaaggtgctg
    20221 ttccaaaaga tggaccatca gctggtataa ctatggctct tgcagtaatc tcagcactta
    20281 ctaaaagacc tgtgcctgga aatatagcaa tgacaggaga gataacttta agaggtagag
    20341 tcttagcagt aggaggaatt aaagaaaagt tattagcagc tcatagagct ggtataacaa
    20401 aagtattaat tcctaaagaa tgtgaagctg atttagatga aataccagaa aatgtaaaag
    20461 aaaaaatgga gtttgtgctt gtagaacata tggatgaagt attagaacaa gctttattaa
    20521 agagtggtga aaataatgaa aattagaagt tcagagataa ctatgagtgc tgtgaataaa
    20581 tcacagtatc cagcagaagg aatcccagag atagcattgg ctggtaggtc aaatgttgga
    20641 aaatcatcaa taataaatac attgcttaac agaagaaact ttgctagaac cagtcaaaca
    20701 ccaggaaaaa ctagaactat aaatttttac ctaataaata atgaatttta ttttgtagac
    20761 cttccaggct atggatatgc aaagatagct aagtctgaaa aagaaaagtg gggaggtata
    20821 atggaacgat atctagaaag tagacaagag ttatgttcta tattcctact tgtagatatt
    20881 agacatgagc ctacagcaga tgataagctt atgtatgaat ggataaagca ctttggatat
    20941 aactgtgtag ttattgcaac taaggcagat aaaatatcta gaggacaata tcaaaaacac
    21001 ataagtataa taagaaaaaa attgcaaatg gaaagtagtg aaaaagtaat tcctgtttct
    21061 tcactaaaaa agactggtgt tgaagaatta tgggaagaga ttgtaaatca atacaatcaa
    21121 catggatatg aaataacagt agattaaatt taaaatgtta aatattgaca taagatttgt
    21181 ttgaataata aatgtaataa tagttagttt taattatatt aatattaaag caaaatatag
    21241 gatgccttaa aatagtttta atgaactttt tttggcatcc ttattgttta taaacaataa
    21301 atatcagtaa ttttgctaaa tactaataaa ttttagtaag gtatgggaaa attagtataa
    21361 attagttcaa ctaaaaaatt attgacttat gtagtcagca ataaatagca tttaagaaat
    21421 agatttatct gtttttctca taaagaaaac agttagtata agccctataa aacatactga
    21481 tgctgcaatc caataagact gattgctagc tataacatag gctgatgtgt gacttttaaa
    21541 cttttgattg tatgtaagac tttgtgcaaa aaaactacta aaaaatgctg ttccaagagc
    21601 aaatgataaa ttacgaatag ttgcaagaaa actgcctccg tatccctgtt tttgaggagg
    21661 aacatctgct aaaatactac tattgtttgg tgaactaaat gctcctagcc caagtccaaa
    21721 aataactaaa catacaatta aaagatacaa tggagaatct gcttttaaaa gtgccattcc
    21781 aactagtgag atggtcataa atgaaaaaga tgctgttaga atgttttttg caccgtgttt
    21841 atcagaaagt gaacctgcaa taggtgctat aagtaccata ataagtggat gtacagtcat
    21901 tacataacca gccatcatag gagataaatt ttttaattgt tctaaataaa atggaagtag
    21961 aaaagatact gccatttgag gaaaataaca agctactcca atgatattgc ctacagtaaa
    22021 gtttctattt ttaaatagag gtaggtcaat aaagctttgt tcaaatttta cttctcttaa
    22081 tgccaacaat aagaagatta taagtgctgt tatacctaaa tacaatcttg tattaccctt
    22141 ctcattcatt gcaagtaata taagcgtagt gcaagatgcg aaaagtagaa gacctggaat
    22201 atcaaagctc ttgttttgtt catccaaaac aggtttaggc agaaatttaa tgccaagaaa
    22261 gactgctata ataccaatag gtatattgat aataaaaata gaaggccatc caaaatgttc
    22321 taaaattata cctccaatta caggaccact catatttcct ataccaacta cagctccagt
    22381 gattcctata gcttttcctt tttcatttgc tggaaatgca ttggaaacta taccaagccc
    22441 agtagccatt aaaatacttg ctccaactgc ctgaaagatt cttgaagaaa ttaatgttga
    22501 taaattgttt gacatactac acagaaaaga acctattgta aatataaaaa atcctaatgt
    22561 gtatagacga tttgaaccta ctttatcgcc aagttttccg aaaaataaca tagtagctgt
    22621 tacaacaagc atataagctg ttacaaccca ctgaatttgt gacatgttaa ttttaaagtc
    22681 agcagcaatt gtaggatttg cgatatttag tatacttcca tcaagagtag acataaatac
    22741 accaatacag atgataatca atactatcca tttttgtttc atgataacac tccaatctta
    22801 atagatttta taagtacgat ataattgtaa aataaacaac aaaaaaagca cctgtatatt
    22861 aggtaccttg aatgtgtcta atttcatgaa taaatacggt agttgaagga tttgtataga
    22921 ttactcatgt aaaattacat aaaggtactc atatactatt ttcttctacc atccagattt
    22981 tactgtcggc tttggaatat gaccaaatta tgccataagg ctcgcgggct tttaccgccg
    23041 atagggaatt tcacccgttt atgaagaaaa acagatgtat tttgtaatat tattttaaca
    23101 taaaattata atggaaacaa ttggatttaa gtaatatcca gctgtattac aatacaaata
    23161 tttcaatatg gacacttaaa ttgtatttat agatagaata aggcacagta tgagaatcta
    23221 taataattag ataaaattaa cctgttatta gtattagatg gcaagtttta aatttgtcaa
    23281 aatcagaaag ctgtaataaa attcttataa tatgaatatc ttaattaaga gatattgtaa
    23341 attatgagga ggcgctagta tggaaggaaa ggtaaagaag gcattcccgg gtggaaatac
    23401 atcacaaggc tcatattctt tttttgacta tataattcct gaaaatgtaa aaagagtttt
    23461 ttgtttaaag ggtggacctg gagtaggaaa atcctcactt atgaaaaaaa tttataaaga
    23521 gtttttgcat agaggatatg atattgattt atatcattgt ccttctgacc caagttcgtt
    23581 ggatggttta gttataaaga aattaggcgt ggtacttatg gatgcaactg ccccacatac
    23641 tatggaccca aagctacctg gagctttgga tgagataatt aatttaggtg attactggaa
    23701 tacagaagaa ttagaaaaga ataaacaaga aatatcagtt tatgataaag atattagtaa
    23761 ttcatttaaa agagcattta agtttttaaa atcagcagaa ccaatcttta gagacataga
    23821 agaaaaatat tctgattgta tggattatgg aaaagtaaat ttggtcacag aagaatttat
    23881 aaaaaggtta tttgatggag ttaaaagcac aggaaatcta aaaagagaaa agcatttatt
    23941 tggttcagct ataactcctg tagggtgttt agattataca gaaaacattt taaaagatgt
    24001 aaagaaagtc tattatttag atggagaaat aggaactggg aaaactacat tattaaacaa
    24061 agtatataag aaagctattg aaaaaggttt aaatgtagaa gtttatcatt atccacttat
    24121 tccagagaaa attgaaactg tactattaac tgatttagat ataggcataa ctataagtac
    24181 gacttttaaa gaagaagata caattgattt aaatcagttt ttaaatagag aaaaactact
    24241 taaatatgaa gaagatatta aatttgatga aaaagtttta gatgatttaa tttgttgggc
    24301 agtactaaat cttaagagag ccaagtctaa acatgatatt atagaaagtt actatagtcc
    24361 taatatgaga tttgatgaag tagggaaatt aagagataag ttaattaaaa gaattttaaa
    24421 atatgaggaa aacttataaa cgcatatgtt taaaattaac gttagtaata atataatttt
    24481 tagtaaattg gtgtagcttt gaggctatgc cattttactt tttttgctaa aaaatgatat
    24541 taataatctc caaagccatt atttgttttt cataattatt atttcctttc ctaatttatg
    24601 tttttttata atatcacaac aatgtttatt gaaagtttat ataattttaa gaacatctaa
    24661 aaatgcatac ttgatattgg taaattagtg ataggagttc aatgtatgat attatatact
    24721 aaagatgaat tttaatatat aactttgata taataagcat atgtaaatgt ttaatattta
    24781 attaaagaaa attatcatat aaaaataaat ttgtaaggag caatacagta tgaaaaaaca
    24841 atatgtgttt ataatactag caagtactat tttaggtata cttgttggaa ccatatttag
    24901 agattatagc aatgaagaaa cagtttatac aagcaaagac aagtcaataa gaaaagaaat
    24961 aaaaatgaca agaaaatcta taaaagaatt aaccaaagag aaagacaagt tagataaaga
    25021 aattaattca atgaaacaaa atcaaaaaaa ttctgaagaa atatcagaaa tagaaacact
    25081 tagagatagt ctatcatatt cagatgtaaa gggtagtggg ataatattaa gtatagatgc
    25141 tatagatgaa aaaacaggga atatagctaa tagtatagac tataataaga ttcttgtaaa
    25201 agttataaat gaattaaaag taaacggagg aaaatacata gaaataaatg gtcaaagaat
    25261 taatcaatat tcagaagttg tacttgcagg aagccatata aatataaatg gagttcctgt
    25321 agcacaacca tatgaaataa agtgcattgg agataaggag aaactatcta attatgtaga
    25381 taaaggtagt aaatatttag atagcataca aaccaattat ccaatgaaaa taagcataaa
    25441 aactgaagaa aatatctcta tgaaaaaaat gaatatacct aataaattaa agtatataaa
    25501 gggtgattaa gattatatta gaggaaagca gtttgccaat gttaatacca gaaaaacttg
    25561 caaaaaaata taatgtattt ccagttgagt ttcaagatga taatttagtt gtagaaatag
    25621 aagatgagga tatttatgct ctacaggact tgagattagc tacaggtaaa gaaattatat
    25681 tgaagaagga gaaaaaagaa cttataagtg aaaagattga aaaatattat aatagctttg
    25741 aacttgatga ggattatgca aaaaaattat ttgaaaatct tttagaaaaa gctataaaag
    25801 aaaacgcaag tgatatacat atagaaccat ttaaagaaca tttaatagtg agaatgaggg
    25861 tagatggaga actaaaggaa gtatcaaagt ttttagttga tgtctatcca tcattgtcta
    25921 cggttataaa attaaaggca tcaatggata taacagaaaa aaggcttcct caggatggta
    25981 gggtggatat aaagttaggt ggaaatttaa ttgacattag agtttcatca ataccaactg
    26041 tctatggtga aaaaatagtt ttgaggatac ttaatagaga tactttttta aaagacaaat
    26101 tagaacttgg tttttcagag gaagctataa aatctataaa tcgtataata aataaaagag
    26161 caggaatatt attagtgaca ggtcctacag gtagtggaaa gactacaaca gtatattcct
    26221 tattaaatga tttaaaagga ataaataaaa atattatgac tatagaaaat cctgtagagt
    26281 ataagatgga tggcataagt cagattcaag taaattcaaa agtagggttg agctttgatg
    26341 taggtcttag agcgatatta agacaagacc cagatataat aatggtcgga gaaataagag
    26401 atgcagaaac agctaagata gcagtaagag cagctataac aggtcattta gttataagta
    26461 ctttacatac taatgatgca gtatcttcca tagctagatt actagaaatg caaataccac
    26521 catatctttt aaatgcctct cttataggtg ttatatctca aaagttggtt agaaaagtct
    26581 gcaatcattg tagccatgag attatgataa aagataattt tagtggagat gtaaatacta
    26641 aggtagctgt agggtgtgaa aaatgtaatg ataaaggata ctttggaaga acagcaattt
    26701 atgaaatctt agaaattaat gatgatataa agacatgtat aagaaatatg gaggattcta
    26761 gtactattaa agatgtggca caaaataatg gaatgataac atttgaagat agttgtaaaa
    26821 gactaataaa tgaaaaaata acgacattag aagagtgttt agtagtaaat gaaatgattt
    26881 aactgaattg tatggttaaa cttcgtttgg tcagttgtct ttatttaatc tattttaatt
    26941 aagatgtttg agtataaatt atatttaagt gaaataaaat taaatgcatt aaattagaaa
    27001 atattaagtt tatttaattg taattaatat aatttggaag gtattatagt acatgagatt
    27061 atctaaaaat atattttata atttagaaaa gttttattta gtattaaata gaatcagtga
    27121 taaagaaatt agagtaattt gcaaagagat aggcatattg ctggaatctg gatgtgaaat
    27181 tacaaaaata tttgaaatca ttgagagtca gtcaagtaaa aaagtaaaga atttgcttag
    27241 tatagtatca aatcatattc aaaaaggtaa ctcaatagca gaatcatttc aaataacagg
    27301 tatattttct aaattcttta taagtatgat aaaagcagga gaaactagtg gaaacttaga
    27361 tataataatg agtgatttat ctaattatta tgataaagaa tacaaattaa aaatgaaaat
    27421 aataactatt tcaatatatc caataatact tataatatta tctatcttat caatgttgtt
    27481 tatattcgta tttgtaatac ctaattttca ggtggttttt accaacaatg gaattgaacc
    27541 acctttaata actagagttt taatgggaat atctacagtg gttacaaata acttagctta
    27601 tataatattt agttttatat tgttttcaat aggaagcatt tatttcttta taacaaatga
    27661 taatataaaa aaattgatta attcgttaaa attcaaaata ccttttataa aaaaaattaa
    27721 tcagttagta gctacaacaa gattttcaag ggcattgtcc gttttaatta gcagtggaat
    27781 acaaattgtg gaagctatag acatgtcagc tagtgttata gacaacgaat ttatttatga
    27841 aaggttaaag atttccaata attatataaa aaaaggaaat agcataggta aatctttgaa
    27901 tttagcaaat gtttttccaa aattatttat ttcaatggca aatacaggag aagaaagtgg
    27961 gaaactagat aaaagtctaa atgtaataaa taaattttat gaaaatgaat taaataacaa
    28021 aatagagcaa ataatgaaat ttatagaacc tatgctaatt gtatttatgg gattaattat
    28081 tggtactttt attttagcaa tggttgttcc tatgtttgat gcaattacta atttttagag
    28141 agaaaaataa attttattag atgaaacttt tgaaaaggag agaatattta attgataaat
    28201 ttgataaata aaaaacgaaa aggatttaca cttgttgaaa tgattgtagt agtaactatt
    28261 ttaggcgtta tatctagtat agcattagtt aagtatagta aggttcaaga aagtgccaaa
    28321 ttaaatgcag actatacgaa tgctgctaat atagtaaccg cagctagcat ggcaattaat
    28381 gatgatgaaa agacaataga ctctctaagt gtagagacat tgaaggaaaa gggataccta
    28441 aatactgttc cagttcctca gagtacatca ggtaaattcg aacttgtcat aaatgatagc
    28501 ggaacagata taagcgtaaa tataaattct aagcaatttt atccaaaata atattttata
    28561 ttgattgcta taagttttaa aattattttt atttaagaag gagatggcta taattaaaaa
    28621 aattctaaac ttagaaataa atgatgatta tataaggctt gtatttttaa gaaaaggtca
    28681 taaaaaagtt tttatagata aatcaattat aaaagagatt aactttgata taaaaaatga
    28741 tagtaattcg attgagaaga ttaagtatat agaagatgta gttagatttg taaaagagga
    28801 gattggtaaa tcaaaaatat tatataaaaa tttatacttt aacattcaaa ttcaggatat
    28861 agtcattaga aatgttgaag ttctaaatac taagaaaaaa agagacatat taagcatgat
    28921 tgaatttgaa ataacacaat acattccaat aaacataaat aactattcaa taaaatataa
    28981 agttataaat tctaaaaatg ataaattgaa tatacaagta attcttttcc caaagagtat
    29041 tattcaatta attaagaaca tttcagaaaa tctaaatatg aatccaaaga ctataaatat
    29101 aaattttgat atattacaaa agcttataaa cttaaattta atagagaatt tttcagaaga
    29161 aggggtattt atagaatgta aaaaaagtca gttaattata aatataacaa aagataaaaa
    29221 aataagagaa acatacttgc tatcaaaaga gggaaaatca tatgagttca taaaaagatt
    29281 attaagggat tttaagtatg tttattacta tggtatagaa aattgtttta ttgaagaata
    29341 ctttaaatat acagataaat ctattgaagt aaatccatta agattaaaag ataaagtaaa
    29401 agtagaaaat aattttgatg cagatttaga agacaatagg ataaattata taagtaatat
    29461 aggaatgatt atttaagtga aagtggataa aacaataaat tttcttaatg gatttaaaaa
    29521 aactaaacca tgtaataaaa tctataaaat aataatagtc ttaattttaa cgtgtatttt
    29581 atttaaatca tttatgtatg tgaaatctat aaatgaactt gaaaattata taagtcaaaa
    29641 taagctagtg gataaatcaa agaacacttt accaacaaca gacaaaacta gagagaaatt
    29701 ttctaaaata aaacttaatg aaataaaaaa aatatatgaa acaattggaa taaatagtat
    29761 tactaaaata tcaatagata aaaataagct agagatggaa ggtgtgtgta gtgatttaga
    29821 tgttttaaac aatttaagga aaataaagta cataaaatat tattacatag acagtattgt
    29881 aagagatgga gaatattata gatttagaat aaaaagttag atgggtgatt atttttgaga
    29941 ttaaataata gagaaaaaaa tctacttaaa atattgttaa tattagttat tatattggca
    30001 agtgatatag cagtaagtta tttggaagaa aacctaacta gtttgaagaa tgaaaagcaa
    30061 aatataataa ataaaataaa taatcaagat aattatcaaa taaaggataa taagcatata
    30121 gatgataaat atatagaaag tgttgaatca aataaaaatg aagaattaaa aaataatttt
    30181 aataactcat ttttagataa aaaaacaaat aaagatatag tgttattagt agaaaatgaa
    30241 ctgagagata ttgtgaatgt agaatatatt aatcaagaaa gtagtttaga taatcacaat
    30301 aaaaaacaag ttaatttaga aataaaagtt aatggagaac ttgcaaatgt gttgaaactt
    30361 gaaggtgtaa tgaagaagtt aagactaaat aataaacttg aaaagataga aattaataaa
    30421 ttaaaagtca ataattttga tgttcaaaat attccaaatg aatataaaca agaaaaagac
    30481 aatattgttg aatgtaaaat gacagttaga gtcatataac aaaaaagaat agataaagta
    30541 aaaaactatt atcaattaat aaaattttat agaggaatta gttttacgat aattcatttc
    30601 tacaagaggg attgttgatg attttatcga aatcctaatt aaagttaata tattgtacca
    30661 aaatataagt gaatttaata gaatatttac ttatactata gattgattaa taaaaaaatg
    30721 gtataataac aaattgtact ttaatttaat aataagggag tggaagtcat attgttaggg
    30781 agaaatgagc tatgcccttg tggaagtggc aaaaaataca agaaatgttg tttaaataaa
    30841 gatattgttt cagaaagagc atgcagaaaa actgaacttt cacaaaaaca atatacagat
    30901 ttatattcaa agttatatga ttattcaaga caggataagt ttaaagaaga atgtgaaaaa
    30961 gctaaagaaa tgttttatat aatgcaaaat gatagtgtaa atgaaaaatt tgaaagattt
    31021 tttaatactt atttaataca agaccacata atggaaaata aaaaggttat gacagttggt
    31081 tttcttgaag aaaatggtgc aaacctaagt caatcagaag tatcaatatt aaaaagtatg
    31141 tttgaatcat acgtaagtat ttatgaggta aaagaagttt ctgctggaaa aatagtatta
    31201 aaggactgtt taagtggaaa tgaactttgt acagaagatg taaaacttct gagaagtttt
    31261 aaaatcggaa gttgtatgat agctagaata gttgatattg aaggaactaa catattgata
    31321 gatataacta taagtatatc aaatgaggtt aaagatataa ttttaaagga tattatgaat
    31381 ctattcaatc aatatcaaga tgtatacaaa gatatgaagt cgttcttaat ttatcataca
    31441 catatactat acaagtatat acaacagctt ttagacccaa gtatagctga atatttaaag
    31501 agagaaagag ataaaaacga tacaaaagtt aattcagaaa atgaattagc tgaagaagaa
    31561 tgtaaagttt tagatactct taaagaaaat gtagaaaaag aaaatataga tgcatgtata
    31621 gagttttgga atgaatataa gaaaaatcat gaaaatataa aaggagctga aaatggttgg
    31681 gcagctgctg ttgaatacta tgttaaaaaa gaagcaggac aacctataac tcaagttcaa
    31741 atatcaaaga aatacgatat aagctctagt acactaggaa agagatacaa ggatttaaaa
    31801 atatcttaat taattataaa aagctttaag tcatagataa agaatctata gtataatata
    31861 tgtataatgt ttagaaagag gtattaataa tgagtcttag tttaggaaat gcaattgctg
    31921 tatcaacgaa ctttataaaa tctaacccag ctgtacctgt gtacaacgga gaagaaatat
    31981 atgcagttat ttatcatata gaaataggat ataataatgg tgtatcacct gataccaaac
    32041 aatatataat aatagactat aaagagtttg atttatctat ggataaaaaa aatgaactaa
    32101 taaatattgc taaagagtac tgtaaatcta aaggtattgt agaaaacgaa aaccttgaaa
    32161 taaacctttt agacaatgaa gaagcagaga acttcttaga aaaggtgttt aataatatga
    32221 aggttataag aggtcttata actaaaaaag gataaataaa tggactatat aggaatagaa
    32281 aatataacac cttatgaaaa tacatatgaa tttagtgtat atgaatatga tgatgaaatc
    32341 accttaggta gtgaaaagtt atatgtatgt gaattgaggg ttgtattgat taaagttaat
    32401 tctctgtatg ttgaaagatt gcataaatca gttgaagcaa tggtcttagt aaaaaacttg
    32461 aaaaaagatt tagataaaac acttgttgta aacaaaataa agaattttgt gctagatgag
    32521 atttgggtag aaaatctagt aaaagagaat atagaagtta tatttgtaga aagctaggaa
    32581 taaccctagc ttttttatat tacaatctaa gagaggaaaa caatatgtta agagtatcta
    32641 atgtaaaact tggaattgat aaagatatat ctttattaaa agatgttata cttaagaagc
    32701 taaaaataaa agaaaaaaac ttagtcaaat atagtatata taaagaatct atagatgcta
    32761 gaaaaaaagg aaaaatggaa tttgtttatt ctgtagatgt agaagttaaa gatgaaaata
    32821 aaatacttaa ctcaaaaata aaagatgtaa caaaaataaa ggaaatgaga tacgtaaatg
    32881 tacctgtggg tgataaaaaa ttaaagaata atcctttggt aattggtagt ggaccagctg
    32941 gtttgtttgc aggtctgctt ttagctcaaa tgggttatga accaatcata cttgaaagag
    33001 gtcttgatgt agacagtaga accaaagata taagtgattt ttggacaact agaaaattaa
    33061 aaaataattc aaacgttcaa tttggagaag gtggagcagg aactttttcg gatggtaaac
    33121 ttacaactag aataaaagat atacgatgta gaaaagtatt agaagagtta gttcagtttg
    33181 gagcaccaga tgaaatatta tactcacata agccacatgt tggaacagat atattaaaaa
    33241 atgtagttaa aaatataaga aatgaaataa taagtttagg tggtcaagtt agatttaatt
    33301 caaaagtaac agatatagtt gtatcaaatg attctataca atctgtaata ataaatgata
    33361 ctgaaaaaat agatacagat acaattatat tagctgttgg acatagtgca agagatactt
    33421 atgaaatgtt gtattcaaga ggtataaaaa tagttcaaaa accttttgca ataggagcta
    33481 gagttgaaca tccacaagat ttaataaata aagcacagta caaggatttt tataatcatc
    33541 ctaggttagg agctgcagat tatagactta tagagcatgt ttcaaatgga agaacagcat
    33601 atactttttg tatgtgtcca ggaggtagtg tcatagcatc tgcatcaaat gattttgaaa
    33661 tagttacaaa tggtatgagt gaacatgcaa gagataaaat aaatgcaaat agtgcatttt
    33721 tagtaaatgt aacgccagaa gattttaaga gtgataatcc actagcaggt gttcatttcc
    33781 agcagaagta tgaaaaatta gcttttgaat taggtggaaa aaattataat gcaccagttc
    33841 agcttgttgg agattttata aacaataaga cttctttgaa tttaggaagt gttgagccat
    33901 catatagacc aggatataca cttacaaatt taagtgaatg tttgcctagc ttcgtaacag
    33961 agactatgag agaagcgttt atatctcttg ataaaaaatt gaatgggttt gcaatgtatg
    34021 atgctgtttt gacgggagta gaaactaggt catcatcacc aataagaata gtaagagatg
    34081 aaaatacttt agaatcagtg agtattaaga atctatatcc atgtggtgaa ggtgctggat
    34141 atgcaggtgg tatagttaca gcagcagttg atggtataaa atgtgcagaa aaaataattc
    34201 aaaaatacac caataataca aaaatttaac ataaacttaa tgatttttta atatttaaca
    34261 tgaatttaac attatcactc ctataatttg ataaaataca ataggttata aaatttttag
    34321 atacagtaat ctctttacaa ggaggaaata atttgaacat tataataagt ctaatgggtg
    34381 gattaggtct gttcttatat ggtatgaacc ttatgggaga aggtcttcaa aaatctgctg
    34441 ggacaaagct aaaaaagata ataaaactac ttactagtaa cctatttatg ggagttttgg
    34501 taggtacagg agttacagca gtaatacaaa gttcaagtgc tactactgta atggttgttg
    34561 gtttcgtaaa tgcaggaata atgactttaa aacaggcaat aggagttata atgggtgcta
    34621 atatagggac tactgttaca gcccaattgg tatctttcga cttaactggt atggccccag
    34681 tagcattagg tgtaggtatt atactttatt tatttggaaa taagcctaga ataaaaaata
    34741 tagccgaaat acttataggt tttggtatat tatttacagg tatggatttt atgaagatgg
    34801 cagtggagcc actaagagac tatcaaggat ttactgactt acttgttaca tttggtagat
    34861 atccactact tggattatta ttgggatttg ggattacagc aataatacaa agttcaagtg
    34921 catctatggg tatgttggtt gcactggcag cagaaggtct agtgccatta tcagcagcac
    34981 tgccaattct ttatggccaa aatattggta catgtgtaac ttcactgtta tcaagtattg
    35041 gtgctaacaa aaatgcacga agagcagcta tgatgcatct aatcttcaat gttttaggaa
    35101 cggtgatatt cttaatattc ttgaataaac cagtagtaag catggttaca agttgggacc
    35161 caagcaatgt tgcaagacaa atagccaata cacatacatt gtttaatata atatctgtat
    35221 tgatactttt accatttaca aatcttatta taaaactagc tataaagctt gttcctgata
    35281 gagcaggtga tatagatgaa gatgaaacta aaactattaa atatatagat gatagaatga
    35341 tagaaactcc ttctatagct cttgcaaata cgataaaaga agcacttcgt atgggtgaga
    35401 aggcgaagga aagcctaaat gcatctatgg aagctttagt agaacattca actgaaaaaa
    35461 tagataagac atataggcga gagagattaa tcaatgattt acagaaagct attttaaatt
    35521 atttacttaa actttcaaaa gcacctttag atgatgattc aagagaagtt gtggatactt
    35581 tgtttaatac agtcaatgat atagaaagaa ttggtgacca cgctaaaaat atagctgaat
    35641 tatcgcaagt tgcaatagat tcaaatattt ctttctcaga agaaggacaa tctgaacttg
    35701 atgttatgta taatagagtt gtttcagcat atacttatgc attagaatca atgagaactg
    35761 ataatgttga tttagcttgt aaggttataa aaattgaaga acaagttgat ataatggaga
    35821 aatcttgtag agctaatcac atgtatagat tgaacaataa tctatgtagt atagaaaatg
    35881 gagttattta tttagacgta ataagtaatt tagagagaat atctgaccat gctgtcaata
    35941 tagcacaaca agttatagca aagagattag gaaatgatta aaaataatta atttaaaata
    36001 atataaaatt taaaatagag gataaataaa agagaaactt taaatgatac gtttagagtt
    36061 tctttttttg taaatttatt tgactaaaat ttttaaattt atacatagtt atagcattta
    36121 gaagaatcga tttcctgata ataaatgatg agatatgtat gattttgtgg caaactttta
    36181 aaaaaaacac ctatattttg tgttataatc tctttaaaac gaatatgctg aaaagaggga
    36241 tttaaatggg aaaaataaat tttgattata gtaaagctac agagtttttt tgtcaaaacg
    36301 aaatagatgt catgcaacca tatgtagatg ttgcgcacga catgttacat aataaaactg
    36361 gtttaggaaa tactttttta ggatggatag accttcctaa aaattatgat aaagaagagt
    36421 ttgacagaat aaaaaaatct gctgagaaga taaaatcaga ctcagatgtt cttttggtaa
    36481 taggtatagg aggctcttat ttaggttcga gagctgctat agatatggta agtcattctt
    36541 ttaggaatgg acttaaaaaa gagcaaagaa aggctccaga agtatatttt gtagggcata
    36601 atataagctc aacatatatt atggatttat tagatataat tgaaggaaag gatatttctg
    36661 taaatgtaat atctaaatca ggaactacta cagaaccagc tctggctttt agaatattta
    36721 aagattactt agagaaaaag tatggtaagg aagaagcaag aaagagaatt tatgcaacaa
    36781 cagatgcaag taaaggtgca cttagacagt tagctacaga agaaggatat gagacttttg
    36841 taataccaga tgatgtaggt ggtcgttttt ctgtattgac agcagttggg ttacttccta
    36901 ttgctgctgc tggacttgat attgatgcta tgatgaaagg cgcaaatgat gctagagaag
    36961 catttcaaaa tcctgactta aaaagtaatg atagttatag atatgctgtt gctagaacaa
    37021 ttttacatag aaaaggaaaa gatgtagagt tattagtgaa ctatgaacct caactacatt
    37081 atgtaagtga gtggtggaaa cagctttatg gagaaagtga aggaaaagaa aataaaggtt
    37141 tattccctgc ttctgtagat ttctcaacag atttacattc tatgggtcag tacatacaag
    37201 atggaaaaag gcttttattt gagacagtac ttaatgttga aaactgtaag agaaatataa
    37261 ctataagttc agaggaagta gacttagatg gtttaaacta tttagcaggt aagactgtag
    37321 attttgtaaa tcataaagct tttgaaggaa ctctattggc tcatacagat ggaaaagtac
    37381 ctaatttagt aataaatata cctcaattag atgagtataa ttttggatat ttagtttatt
    37441 tctttgagaa ggcttgtgga ataagtggat atttattggg agttaatcca tttgaccaac
    37501 caggagtaga agcttacaag aaaaatatgt ttgctttact aggcaaacca ggttatgaaa
    37561 aagaaaagga agagctagaa aaaagactta aataatttta aatagttaag gtttaattaa
    37621 gattaaaata atataatgaa atcatcaaaa aggagtttac tcaaaataga tgatttcatt
    37681 ttttttatga aaaattggga aaaatatatt ttgataattt atattatatt aagataggga
    37741 gtggtatgag atgtcaagaa gaaagaaagg gataagttta gtaatattag ttgctataat
    37801 aagttcaata cttagtagtt ttctaacaat aattttagtt aaggacaatt tagtaagtaa
    37861 atctacagga agttctacgc cgatagttgt aaatgatgat ggtaagagtc aaaatattta
    37921 tcaagcagtt gcagaaaaag ctacgccatc tgttgtaggt attacaacaa ctagtgttga
    37981 tactagtaat atgtttgcaa taccaactga aacacaaggt gtaggaactg gaatcatagt
    38041 tgattcaaat ggatatatac ttactaattc tcatgttata tcagatggtc aagctacaag
    38101 tgtaaatgta ttattcaatg atggttcaac aacttcaggt aaagtagtat ggtttgacca
    38161 acaacttgat ttagctatag ttaaggttga taaaactgga ctaacaccag cagaatttgc
    38221 tgatagtgac aaagtaaaag ttggagatat atcaatagca ataggtaatc ctcttggatt
    38281 agattttcaa aaaacagtta ctcaagggat aataagtgga ttagatagaa ctatacaaac
    38341 agaaaaaact aatatgactg gtttattaca aactgatgca agtataaatg caggaaatag
    38401 tggaggacca ttgttaaatc aaaaaggtca agtaattggt ataaacacag caaaagcttc
    38461 tcaagcagaa ggattaggat ttgcaatacc aatcaataca gctaagagta tagttgaaga
    38521 agtaatcaaa aatggaaaat atgaaaaagt aactttaggc ataaaaggta cagatgtatc
    38581 aaattatgaa gcagcaacag gtacaaaatt aagtactgat aaaggtgttt atgttgcaga
    38641 agtaatatct ggttcatcag cagaaaaagc tggagtaaag gtaggggata ttataactaa
    38701 agtaggagat acagatataa caggtatgaa tgatttaaat aaaaagttgt atactttctc
    38761 aaaaggtgct agtacaaaaa taacagtaaa tagaggcgga aaagcagtaa caataaatgt
    38821 gaatttctaa atatgaccag aaagatagag actacatggt aaagtgtagt ctctattttt
    38881 atttaatttt tatagtatat acatctaaat taattaaaat gatgtattgt ttatagttta
    38941 ttgctatatt ttcttataaa aataaattta ataaatattt aaatttaatt tacaaaaaca
    39001 atataaatat cataataaaa taaaattttt taatattact aaaaatatat agacaaaata
    39061 ataacaaata tagtattatg taattgtaga aatgcttttc ataggtatat tctatgaaga
    39121 tgaattgcat aaaaatgaaa aaatggaatg gatattattt aggggtgaaa aatcatgaat
    39181 ccattagtta taaacttaca aaaatgtagc atacatgatg gaccagggat aagaagtaca
    39241 gtatttttta aagggtgtcc acttgaatgt gtttggtgtc ataatccaga gagtcaaaca
    39301 tatacaaaac aagttttgta taatgaagaa agatgttcaa aatgtgaagc atgtataaat
    39361 atatgccctc ataaggcaat ttacaaaggt gaaacaaaaa tatgcttaga ccaagataag
    39421 tgtgagtttt gtgaaacttg cttagactat tgtgtaaaca atgcaagaga gattgttggt
    39481 caagagtatt ctgtaagaga tttggttaaa gagatagaga aggatagaat tttctatgaa
    39541 gaatcaggtg gtggagtaac tctatcaggt ggagaagtaa tggctcaaga tatggacttc
    39601 atttgtggag taataaacat gtgcaaatca aagggaatcc atgtagcaat tgatacatgt
    39661 ggctatgcaa agtcagaaaa ttatgaaaga gttgctaaat gtgcagattt gtttttatat
    39721 gatattaagc ttattgatga agataagcac ataaaattta caggaaaatc aaatgactta
    39781 atactgaaaa atgttaagat tttaagtgaa ttaggggcta acataaatat aagaatacca
    39841 ttaattgtag gagtaaatgt agatgatgaa aatttagagg ttaaaaaaat gatagagttt
    39901 ttaaagcctt tgaatataca agcagtaagt cttcttccat atcataatat tggtaaacac
    39961 aagtatgata aaatttataa aaagtatgaa ggagaagaac tacaaagacc atcagaagaa
    40021 aaattagagg aaataaaaag actgtttgaa gcaagtaatt tcaatactaa gattggaggg
    40081 taataaaatg gcaagaggaa cttttgagag aactaaaaaa ttaagagaag aaagtattaa
    40141 tgcagaacca cacatatcta ttgaaagagc agtacttatg acagaagctt ataaaaaata
    40201 tgagggaagt gtagagattc ctgtacttcg tgctttatca tttaagcatt atattgagaa
    40261 tagaactctt agcataaatg atggagaatt aatagtaggt gaaaaaggtg attctcctaa
    40321 tggagcacca acatatccag aaatttgttg tcatacaatg gaagatttag aagtaatgca
    40381 caatagagat ataataaatt ttagcgtatc agaagaagct agaaaaatac ataaagaaga
    40441 aataattcct ttttggaaaa agagacaaac aagagataag ataataaatg caatgacacc
    40501 tgagtggtta gcagcatatg aagcaggtat gtttacagag tttatggaac aaagagcccc
    40561 aggacataca gtttgtggag atacaatata taaaaaagga tttttagatt taaagaaaga
    40621 catagaagca agattaaaag agctagattt tcttaatgat ttagatgcat ataataaaaa
    40681 agcagattta gaagctatgg caatagcttg tgatgcaatg gttatattag gtaaaagata
    40741 tgctgagaaa gcaagacaaa tggcagaaga agaaacagat gaagctaaga aacaagattt
    40801 attactaatt gctgaaactt gtgatgttgt accagctcac aaaccagaaa cttatcatca
    40861 agctatacaa atgtattggt ttgttcatat aggggttact actgaactta atatatggga
    40921 tgcttttact ccaggaagac ttgaccaaca tctaaatcct ttctatgaaa gagatgtaga
    40981 aaatggaata ctagatagag atagagcaca agaattactt gaatgtttat gggttaaatt
    41041 taacaatcag ccagcaccac caaaagttgg tataacatta aaagaaagta gcacatatac
    41101 agattttgca aatataaaca ctggtggaat aaatccagat ggtcaagatg gtgttaatga
    41161 agttagttat ataatacttg atgttatgga tgaaatgaaa ttgatacaac ctagttcaaa
    41221 tgttcaaata agtaaaaaga ctcctcaaaa attcttaaag agagcttgtg aaatatcaag
    41281 aaaaggatgg ggtcaaccag cattttataa tactgaagca atagttcaag agcttatgga
    41341 agctggaaaa actatagaag atgcaagact tggtggaacc agtggttgtg ttgaaactgg
    41401 atgttttggt aaagaagcat atgttctaac tggatatatg aacattccaa aaatacttga
    41461 attaacatta aataatggat atgacccaat tagtaaaaaa caaataggta ttgaaactgg
    41521 agaccctaga aatttcaaat cttatgagga actatttgaa gcatttaaaa agcaattaca
    41581 ttatatgatt gacataaaaa tagaaggaaa tgctgtgata gaaaatatat gtgcaaagca
    41641 catgccttgt ccacttatgt ctacaatagt tgatgattgt atagaaaaag gcaaggatta
    41701 ccaaagaggt ggagcaagat acaacactag atatatacaa ggtgttggta taggaactat
    41761 aacagatagt ttaacagcaa taaaatataa tgtatttgat aagaaaaaat tcgatatgga
    41821 tacattgctt aaagcacttg atgctaattt tgaaggatat gaagctatac ttaatttagt
    41881 ttcaaataag actcctaaat atggaaatga tgatgattat gctgatgaaa taatgcaaga
    41941 gatattcaat gcatattaca atgaggtaac tggtagacca actgtttgtg gtggagaata
    42001 cagagtagat atgttaccaa caacttgtca tatatacttt ggagaaatta tgggagcaag
    42061 tccaaatggt agactttgtg caaaaccagt ttcagaggga atttcacctg aaaaaggtgg
    42121 agatactaat ggacctacag cagttataaa atcttgtgct aagatggacc acataaaaac
    42181 tggtggaaca ttattaaatc aaagatttgc accatcagta gttcaaggag aaaaagggtt
    42241 agataatatg gctaatctag taagagcata ctttaatatg gatggtcatc atatacaatt
    42301 taatgtattt gataaaaatg tactgttaga ggctcaaaag aatcctcagg attataaaga
    42361 cttaatagtt agagttgcag gatatagtga ccatttcaat aatttaagta gaacactaca
    42421 agatgagata ataggaagaa ctgagcaaac attctaggtt tgcttgtaaa taaaacttct
    42481 tactatataa aatcgtaact gatataataa agtataaatg gtttgattcc atttttaaaa
    42541 ttttagatta attatgagct aactttctaa ataggaagtt agctcgttaa gtttaatgaa
    42601 aaaatcgtta aatttatatt tagtaaatca tacacatatt agtaagtaat acatacaaaa
    42661 gaataattta ttatagttat aataaggtta tattttataa aaataagata attttaagca
    42721 gttaaaataa agtcaattta gctactttaa aataaaacta atagaaaaaa ggagatttga
    42781 tatgaaaact ttaggattta ttggttcagg gaatatggga agtgcaatga taggtggcat
    42841 agtacagtca ggattagtaa aggcagaaga tataactgta tcagatttaa gccaagttgc
    42901 cttagataaa gtaaaagaag aatttggagt aaatactaca actgattcaa aggtagctgt
    42961 tacaaattct gatgtggtaa tagtagcact taaacctaat atatgcgaaa aggctttaac
    43021 tccacttaaa gaattaatag atgcaaataa aataatagta tctatagcag caggtaaaac
    43081 tatagaaagt ctggaaagtt ttataggttc agataaaaaa atagtaagag taatgcctaa
    43141 tactccagcc cttgttggcg aaggaatgtc agctctttgt aaaaatagta atgtaacaga
    43201 tgaagagtta aatatgatta aggctctgtt tgaatctttt ggtgaggcag aaatagtatc
    43261 agaatattta atggatacag ttacaggtat tagtggttct tctcctgcat atgtatttat
    43321 gtttatagaa gctatggcag atgcagcagt attagctgga atgccaagac aacaagctta
    43381 taaatttgca tctcaagcag taatggggtc agctaagatg gttcttgaaa ctggaaaaca
    43441 tccaggtgaa ttaaaagata tggtttgttc tccagcggga actactatag aagctgttaa
    43501 ggtattagaa gaagaaggat ttagagcagc agttataaaa tctattgttg cttgtataga
    43561 taagtctaaa gatatgagta aataacagat gtaattaagt ctactatatg gtttcttgaa
    43621 ttgttttttt agtgcttatt tatatctata aataaatgta tttggtataa gtaaaataag
    43681 agtaatttac tataaaaagg aatttaatga ttgagtgaga atttattact taaatattaa
    43741 taat
//
LOCUS       DBGJFF010000019        43545 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.019, whole genome shotgun sequence.
ACCESSION   DBGJFF010000019 DBGJFF010000000
VERSION     DBGJFF010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 43545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43545
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.019"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            200..1417
                     /gene="ilvA"
                     /locus_tag="ACOIAF_001074"
     CDS             200..1417
                     /gene="ilvA"
                     /locus_tag="ACOIAF_001074"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HGZ2668095.1"
                     /translation="MMNVTLTDVKEARETIKNIVKRTDLLESVKLSEKTGANVFYKCE
                     NLQKTGSFKLRGACNKIASLTDEEKANGVIASSAGNHAQGVALGAKMTGIKSTIVMPA
                     TAPLAKVSATKGYGAEVVLNGAVYDDAYAKAVEIQKETGATFLHPFNDKYVIAGQGTI
                     SLEIFEQLDNKVDTILCPVGGGGIISGVAVAAKALNPNVKIVGVQTANIPSMKESIKN
                     GKVTTAFNDTTIADGIAVKTPGDLTFEIINELVDEIVVVEETEIAESILFMMESQKIV
                     SEGAGAVCTAAILSGKYVPAKDENVVCIISGGNIDINTLYRIIGVALAKEGRRYSFST
                     IMEDKPGNFAELTRIISENGGNILSANQGKLSAGEALGKQSAEFILETIDYDHIARIK
                     KAIEEKGFKIIEL"
     gene            1512..1892
                     /locus_tag="ACOIAF_001075"
     CDS             1512..1892
                     /locus_tag="ACOIAF_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HGZ2668096.1"
                     /translation="MKHEVIHTNDAPAALGPYSQAIKAGNLLFVSGQVPLVPETMEVV
                     EGDVQAQTAQSLKNLKAILAESGADFSNVVKTTVFIKDMNEFGAINEVYAEYFGENKP
                     ARACVEVARLPKDVKVEIEVIAVL"
     gene            2176..2658
                     /locus_tag="ACOIAF_001076"
     CDS             2176..2658
                     /locus_tag="ACOIAF_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HGZ2668097.1"
                     /translation="MLNFFKKNKSYKLHAVVSGNSINIEKVNDSVFSKKLMGDGVAII
                     PNSDVVVAPCNGKVTVLTESKHAFGMVSDEGVEILVHIGIDTVSLQGEGFKNEVSQGD
                     TVKKGSPIISFEREKINSQGIDCTTIIIVLNHSEFSEINCMVENEVVAGQDTVIEIMK
                     "
     gene            2679..3494
                     /locus_tag="ACOIAF_001077"
     CDS             2679..3494
                     /locus_tag="ACOIAF_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HGZ2668098.1"
                     /translation="MRVVKIFNNNALSTVTNDRKEAILLGLGIGFNKRPGDKVNEDKI
                     EKIYYVQDHMQTKFLELLKNVSPEVMDASQQIISLIPGNEGKFNNKGILSLVEHISFA
                     IDRMKNNVFLPNLMLLDIKMMYSKEFELGVKALEIIYRVCHIHLPEDEAGYIALHFVN
                     LQSNDNLAYDTLKFVKGSIDLIKECYGLELDESSLSTLRFRTHLKFLAQRIFKNEICQ
                     DDKMIEMYDYLINNHPKNKEYLEKLNVYIEKEFRYKLDKPEKIYLLLHLTKIL"
     gene            3547..5097
                     /locus_tag="ACOIAF_001078"
     CDS             3547..5097
                     /locus_tag="ACOIAF_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HGZ2668099.1"
                     /translation="MKEKLLGGLETFAKAMVQPLMYLSSAGILMVVGVLLTNDMLLGV
                     LPFFKWEPIQIFGSLIYNCIMAIINNLAVIFAIGIPAALAKKEKHKAALIGFMSYIIY
                     LTASNTMLTSLGQLAKESKMLGLIGTGQATILGIQVVDTSVFGGIILGYIAGYVFNRT
                     SNKTFKGALQIYSGANFSFICMFFVSILIGVVSNFIWPLAQQGIASLANVIKDTGTFG
                     LFLYGFLERILVPTGLHHLIYTPFQFSDLGGVLKVGADTVSGAYAIVMTEMNLPVERF
                     SDSIYYMATGFTKMFGYIGIGAAFIYTAYKQNKKKVKATIIPLIITAVLASVTEPLDF
                     MFVFCAPLLFFVHAVIAGLFIALLKIFDVTALCGGNLLVSVIMNTTVGVEKTHWPVMM
                     ILGLAQIIVYFVVFSFLIKKFNYKTPGREDESTATGEEQSKQLNLDAGIENIIDGLGG
                     KENINTVENCITRLRVNVKDESKINEDIINLTPNSGIVRKGKDIQIIFGLHVHEVRRA
                     VEDFLEAY"
     gene            5194..6546
                     /locus_tag="ACOIAF_001079"
     CDS             5194..6546
                     /locus_tag="ACOIAF_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HGZ2668100.1"
                     /translation="MIITIVGGGSTFTPGIVKSIALREKELELEEIRLFDIDKKRQDK
                     VAVVVNWILKEDVKTDVKLTVTNCEKEAYTDATFIFAQMRVGKYEMREQDEKIPLKYG
                     CVGQETCGCGGMAYGLRTIQPMIKVIDDVEKYAKKDYWILNYSNPAAIVSEACRKLRP
                     NARIINICDMPIAIIDMIAGSLNINDVHNIRYDYFGLNHFGWFTSIDYKHRDLMEEIK
                     EYIKENKILLPKSYIENLNKSRSTGNRHAVSSWIHVWESVYTMLESFPKYLPNTYLTY
                     YLLSKETMEHSNINRTRANEVMESREKDLFEGVRHYLETGEISEKAFYAGSHGDWISD
                     LAVSIKNDTRSRFLVITENRGAIPNMPYDAMVEIPAYIGKDGPEVIARGPIPLFQQGL
                     MMQQLNSEKLIVEAYIENSYEKALQAFTLNKTIPSMNVAKEILDEMIEVNKEYWPELK
                     "
     gene            6647..7435
                     /locus_tag="ACOIAF_001080"
     CDS             6647..7435
                     /locus_tag="ACOIAF_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGZ2668101.1"
                     /translation="MIFLEEWGDAMNIINKIFWQEDRIGMITNNLEADTHSHCMLQLF
                     LGIEDSIEITVNEKLVKCNCIIVDKNISHSFSARKKVYYSAIIEPTSIYAEQLTSKMN
                     DFGYWICDNDGLEKLRQQGTFLIDNSSKEQYLRFMEMLNNYLNIPITLKHYDDRITEL
                     LNLLRTCNCDNHTISSFADKVSLSASRLSHLFKEQIGIPLKSYILFHQMECAFRELLS
                     GKNVTEASMTAGFDTPSHFAGTVKRMMGMPVSLSLKDSEFLKVY"
     gene            7482..8201
                     /locus_tag="ACOIAF_001081"
     CDS             7482..8201
                     /locus_tag="ACOIAF_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HGZ2668102.1"
                     /translation="MNKEYLEETKMLNYNEPQLKLLVSSKNWLELDDFHKIKSIYEFV
                     QNDILFGYNASDMLSATQVLNDGMGQCNTKATLLMALLRAVNIPCRLHAFDVTKDFQR
                     GATSKLISLLAPKYILHTWVEVFYQDRWIALEGVITDKKYLEAIQKKFFNHGGTFKKY
                     AIATNDLKNTSIDWDGKDTFIQKEAIVYDYGIFPSPDVFFSTHSQHMSKLKNFIYVHL
                     IRKIMTKNVCKARNNYIDKNE"
     gene            complement(8325..9725)
                     /locus_tag="ACOIAF_001082"
     CDS             complement(8325..9725)
                     /locus_tag="ACOIAF_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HGZ2668103.1"
                     /translation="METQSNNKKGNLIIAIVMTGAFISSLSQTLLSTALPNIMSDFKI
                     TADVGQWLTTIYLLIAGIIVPTTAYLINRFSTRKLFITSMSIFSIGCIIALFSNNFST
                     MLIARVLQAMGSGSLMPLLQVIILYLCPEEKRGAAMSLVGITVGFAPAIGPTLSGWLV
                     DSFGWHSLFLFLSPIAILDVILSFILLRNVGETQKLKLDIPSIVLSSLGFGGLLIGFT
                     NQGNYGWTNIATYLPILIGIMSLILFTLRQLKSKEPFLELRVFKNKPFLISTILIMIV
                     YASMMSATLMIPLYVQSVRGFSALSSGSLMLPGAILMVVLNPIAGRHLDKYGPHALSI
                     LGTGCLFLGTLSFAFLGRDTSLIHVSLMYCIRMIGISMVLMPLTTWGIKTLDRELISH
                     ATAINNTLRQISGAIGSAILITIMTSATKKAHMSSNMLSNIHGIDVAFSIAATLAFTG
                     LIVSICFIKRHQIIRS"
     gene            complement(9729..10619)
                     /locus_tag="ACOIAF_001083"
     CDS             complement(9729..10619)
                     /locus_tag="ACOIAF_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HGZ2668104.1"
                     /translation="MTEKQRLIITIAQKIFDQKGFQNTSISDVVKECKMSKATFYKHF
                     ETKESFICEIINYYDEKFLEIIHSINENNDIPASEKLNRKIIAVWKNIFSRTTINTYI
                     RENFSEEQRKATSKLQTKSRANLLNEYKLSLFDNYGDKIEKIIFDLVFLLDALIHQFI
                     YIIHVQKREINVYFIAKFTIQILDLVVENMDNLNPLIEKSMFLHKQEDEVYFSLHNKS
                     LFFKTIQDIEELIKTDTSLLENPKLLEAIKKLYVEGKNQQYDSLIMDAMIAYLEKEDT
                     LRPKVLLLNSIKNQLKKETL"
     gene            10898..11545
                     /locus_tag="ACOIAF_001084"
     CDS             10898..11545
                     /locus_tag="ACOIAF_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HGZ2668105.1"
                     /translation="MKKKICVAIIFALFIVFVEMFIDSSSEGSKIMTAELSKYRKGVN
                     VLSINNIIVANKKYSLPKDYSPQESSEARDAFYKMNKDAQKSGLNLKAFSTYRSYEYQ
                     DRLFKSYVKEHGEKEANRFSAKPGESEHQTGLAFDIGGDDQSCWANKKFNNTKEAKWL
                     YENAYKYGFILRYPEGKENITGYMYESWHYRYVGTEHSKNFAMNNLTLEEYLHIN"
     gene            11869..14508
                     /locus_tag="ACOIAF_001085"
     CDS             11869..14508
                     /locus_tag="ACOIAF_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HGZ2668106.1"
                     /translation="MSYKSTYQEVIKNLDSSLSGLSKERAEELLEKNGANELKEADKV
                     PTYKLFLESFKDPLVIILLIAALVQIFLGETVESIIIFAVIIINSVLGVVQTKKAESS
                     LESLKNLSAPNAKVIRDNKKMTIPAKNLVVGDIVFLEAGDYIPADGRLIEAQSLKVVE
                     GMLTGESEPVLKHTEKISEDVSLGDQKNMVFSGAVVVYGRGSFVVTGTGMDTEMGKIA
                     GLLESAENKLTPLQQKIEDFSKKLGVGIVILALLIFIIQVARNYFMAGNGEMTQIILD
                     SFMFSVAVAVAAIPEALSSIVTIVLAVGTNSMAKKQAIIRKLPAVETLGSTSVICTDK
                     TGTLTQNKMTVVDYYMYGTNNDELDKEKVNYSYHAKLLTMVSTLCNDSHITSDGKEVG
                     DPTEVALINYSSKNDLDYDKLRSKYIRINEIPFDSDRKLMSTVNSIYGDYIMFTKGAP
                     DIVFSRCKYALKNGSKVDITDEIIEEYKHKNEEFSNRALRVLAFAIKDVPEEDFIPSI
                     DDEHDMTLVGIMAMIDPPREEVMDAVKEAKSAGIKTVMITGDHKTTAAAIAKEIGIME
                     EGDLALTGKELDALSEEQLYEKLENISVYARVSPENKIRIVKAWQHKNNITAMTGDGV
                     NDAPALKQADIGIGMGSGTDVAKDASAMVLVDDNFASIVNAVEVGRTVYSNIIKSITY
                     LFAGNLGAIVSIVFAVFAGWSNPFTALQLLFINLVNDSLPAIALGFEKSEKNIMNKPP
                     RHPDESILAGGTLKAVAIRGCIIGVVTIIAQYIGLQVSPELGVAMAFSTLILARIMQT
                     LPARSNNQTVIKLGFFSNKYVIGAVLVCLGLYSLTLIPVLRGIFSIPTTFGLYQLGIC
                     LGLALISSILMELNKLINRSEVK"
     gene            complement(14566..15669)
                     /locus_tag="ACOIAF_001086"
     CDS             complement(14566..15669)
                     /locus_tag="ACOIAF_001086"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HGZ2668107.1"
                     /translation="MKLKINKAIEMQLKKSYAIESGHSEDVFEIDCGEGINTVSYSNK
                     AVEAFNALKFDMIRGYPHSIALKDNIVDYWKDFIALDTDRICLADGSIHVIYLLNRLF
                     IEKGDKVLGYSPQFSEYETDIKMHGATYDYVLLKKEDNFKFNEKEFIEKINPEYKVIY
                     IDNPNNPTGQIIPLSSIENIVKEAAKYDIAVMVDEAYGEYMPKENSAVKLLNNYDNVI
                     ALKTFSKGFGLAGLRAGYAVLPEQLVSPIKKISTPYEVSEISRSIAANLLDDVQFIEE
                     LKEKTKDIKNQLLIPWKNLNIAETSDTVSIMTVEHKNKDIDLQQEFAKLKIRVISGSD
                     FTGLDKNFIRFRMPEEKELPEVIKAFQIIDNIE"
     gene            16030..16851
                     /locus_tag="ACOIAF_001087"
     CDS             16030..16851
                     /locus_tag="ACOIAF_001087"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HGZ2668108.1"
                     /translation="MGYKLIVTDMDGTLLGNNHKVTDENKTALQKVIKSGINVTLATG
                     RAFDSAKCNVDFLKEDMPIIACNGSLIREQNGNIIYSNKIDTRTCLNILDVLDKYDIY
                     YQCNSIDSMLSKKIEGREDRLSVFLGSETEVIVKDDLREEIFKKDILKFVIIEEKNPS
                     ILDEIRKELRKVQGIKITSSWPNNIEVMNEGVDKGNAVKILAEKMNIDREDIIAFGDN
                     YNDIEMIKFAGLGVAMGNAEELIKQEADYVTDTNQDSGVAKAIYKFLELKESLSS"
     misc_binding    16930..17141
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            17228..18544
                     /gene="argH"
                     /locus_tag="ACOIAF_001088"
     CDS             17228..18544
                     /gene="argH"
                     /locus_tag="ACOIAF_001088"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HGZ2668109.1"
                     /translation="MKLWGGRFRKAENQLMEEFNKSFGYDCVLYKKDIEGSVAHVHMQ
                     VKCGLLTEEEGKSITEGLKGILEDVENGKLVLDGEYEDIHSFTEINLIQRIGDVGKKL
                     HTARSRNDQVAVDMRLYAKEKANDLVGLISEFKATIKDVADKNPVMMPGYTHLQRAQV
                     VTFKHHLMAYYSMFDRDEKRIKNAIEILDESPLGCGALAGTTHDIDRSITCEQLGFKK
                     VVDNFMDGVSDRDYLLELMSDFSIIMMHLSRLSEELILWSSQEFGFVEIDDLYTTGSS
                     IMPQKKNPDGAELIRGKTGRVYGNLFGLFTVMKGIPLAYNKDMQEDKEGFFDSVHTLE
                     MCIQIMDRMIATLKVNEDKMKQAVKNGFLNATEVADYLVKNNVAFRDAHGIVGSIVIY
                     CEDNKKAIEDLTLEELQKFSDAFKEDIYDFIDYESILNKGIKKNLK"
     gene            18793..19131
                     /locus_tag="ACOIAF_001089"
     CDS             18793..19131
                     /locus_tag="ACOIAF_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HGZ2668110.1"
                     /translation="MNLLVIGGHERMEKDYMVMAKKKGYKTKVYTTMSSKLNNSIGRP
                     DAVVILTSTVSHKMSRTVESQAKKQDILIVRHKNSSKVAFNECLDMVDECLGNCSKCK
                     LKDKQKQKNI"
     gene            complement(19258..20547)
                     /locus_tag="ACOIAF_001090"
     CDS             complement(19258..20547)
                     /locus_tag="ACOIAF_001090"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HGZ2668111.1"
                     /translation="MKRNLSLLLICLLIFTSFLGSSNISFADNKPAIVAKHAVLMDYE
                     SGKILYNKDGNSKLYPASTTKVWTACLVLKEVKDLNQVIEIKDLPQIDGSSMYLKNGE
                     SFTVKQLLDALLVHSANDAAFVLARYVGGGNVQKFIDLMNSEAKKIGATNTHFNNPHG
                     LPDPNHYTTAHDMALIAREAMNNDTFRQIVKTKSLKFEATKAYPYERYFVNTNKFLTS
                     HDKITYKGQPINIKYDIVDGIKTGYTDAAGKCLLSSAVKDGRRVIVAVFNSTNADLYL
                     DSRILIDYGFDNFKCATIVDKEKYTDTKKVLFTKQHELIYEPKNSYRIFLEKDESQGN
                     YDTKTELNKIDLPIKKGAKVGTLNVYNNGKLENSIDLIAKNNLDSSLPFLTENNVLMT
                     FVKIIAGILVLLVLFIIASNIKKKKRKKARSKKNMKK"
     gene            20627..21331
                     /locus_tag="ACOIAF_001091"
     CDS             20627..21331
                     /locus_tag="ACOIAF_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HGZ2668112.1"
                     /translation="MKKKVKFISFFVLFILIISSIIVYKSLIFKDDIYVVSKKNLPNE
                     KTHVVENEIKGEDNNHNKVTKDIDKNFQNNKITIYISGAVNRPGIVTIESDKRLYDAV
                     ELLGGTTKEADLNGVNLSVRLEDEQHYIIPKIGEATSVTSNDDSNKPNQKNESRGEPK
                     NKSNKNISNESKVNINVATIEELDSLPGVGEATANKILQHREENGQFSSIEEIKNVNG
                     IGDKKYENIKDLICVD"
     gene            21423..22469
                     /gene="selD"
                     /locus_tag="ACOIAF_001092"
     CDS             21423..22469
                     /gene="selD"
                     /locus_tag="ACOIAF_001092"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:21471..21473,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HGZ2668113.1"
                     /translation="MKKEQKKLTEMTTAGGUAAKIGPEVLASVLSQLPKNDNIENLLV
                     GLDTADDAAVYKLNDDMALIQTLDFFTPMVDDPYVFGQIAASNSLSDVYAMGGKPLVA
                     MNIVCFPSCHDMDVLAEILKGGFDKVKESGALLVGGHTVDDKEPKYGLSVSGIVSPNK
                     VLSNATAKPGDKLIITKPIGVGVLNTAMKEGMVEQHIADKVIEIMIHLNKYAAMSFEK
                     FDVNSVTDITGFGLLGHTLEMAKASEVSIEIESKHVPIIEGAIEMAQMGIIPAGMYKN
                     MHYVSKDVEVVGNIEVAVQDILYDPQTSGGLLISVKEELAEELVKDMKLNGAIEAKII
                     GSVVPKGEKYITVL"
     gene            22520..23899
                     /gene="selA"
                     /locus_tag="ACOIAF_001093"
     CDS             22520..23899
                     /gene="selA"
                     /locus_tag="ACOIAF_001093"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HGZ2668114.1"
                     /translation="MLPSVDEVLSDNRIVEIINEYPRSLVLESVREVIDLKRQFILRL
                     KEDASNSVTIEFGEIIESAIERVKLNYSLSLKKVINATGTVIHTNLGRSLLSEDIKDE
                     LWCAASRYSNLEYDLDNGERGSRYSHLTSTIKRLTGAEDVLVVNNNAAAVLLVLSTMA
                     KGGEAIVSRGELVEVGGSFRIPSIMALSGAELVEVGSTNKTHLKDYKEAITEDTNVLM
                     KVHTSNYRIMGFTESVSIEELVNLGEKYKLPVIEDLGSGVFIDLSKYGLSYEPTVLDS
                     IRQGADVVTFSGDKMLGGPQAGIIVGKKEYIEKMKKNQLTRALRVDKLTICALEATLR
                     MYLDETKAIENIPTLKMLTYKIEELEVKANKLFEKITALNLNANINIEDGFSQVGGGS
                     MPLETISTKVISITPEHMNVSSLEKKLRLSEAHIIARVYDNKYVLDVRTIFDDEFDVI
                     VAELRKAFN"
     gene            24013..25896
                     /gene="selB"
                     /locus_tag="ACOIAF_001094"
     CDS             24013..25896
                     /gene="selB"
                     /locus_tag="ACOIAF_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HGZ2668115.1"
                     /translation="MKNVVIGTAGHIDHGKTTLIKALTGRETDTLDEEKKRGISINLG
                     FTYFDLPNNRRAGIVDVPGHEKFIKNMLAGASGIDIVLLVVAADEGVMPQTVEHLDIL
                     SFLNIKNGIVVLTKSDTVDDEFRELVKEDICEKIKGTFLEDAEIIEVDSISKRGIDTL
                     IEKIDKMSDDIEDKNENSPARLNIDRVFSIKGFGTVITGTLIEGKISIEDDLVIYPQV
                     LKTKIRSIQVHGENKDTAYAGQRTAINISNVKVDDIKRGDVLAAPNSLEESMMLDVKL
                     SLVNHLDKGLKHWDRLRLYHGTREILCRAVPLDKELIESGESGYVQLRLEESIVSKKG
                     DTFVVRRYSPMETIGGGVIIDPSPKKHKKFDEKVIEALKIKEKGELKDIIEEYLKRNL
                     KNYPNIKEIMSYSGAHEEDVKRALETLISEDKVFIIGNMYMHINQYNKLKENTIKLLS
                     EYHKKYRLRKGILKEEVRSKIESNFKTREMDILLEKLSTENAIKIENNIVSLLDFEVI
                     LNDKQKEIAKKIEKRLKSCGVSSILTIDEVSEGNHNYAEVLESMIGNKVEKLDDLYIM
                     DKDIYENAKNILINYIKENKEITLGEYRDLIDSSRKNCMIILENFDRNKITKRVENKR
                     ILF"
     gene            26001..28739
                     /locus_tag="ACOIAF_001095"
     CDS             26001..28739
                     /locus_tag="ACOIAF_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation-associated two-component system
                     sensor histidine kinase"
                     /protein_id="HGZ2668116.1"
                     /translation="MKNIIPDVKPDIKEREDMSLLFIIMGSVLLLFIIKDIEKYETLL
                     HAIYTIFLVATGVIIFNTTKFKVDSFSTFLGLIFIATGVLECIYLFNSLGIKTKSIME
                     INITISAITDLFPILGVYLSFKFVKDNKQIYSSVILFIATATLTITSLFIISGICDYL
                     GNGKSQYALGVIISIFIIIISIISGIELNSSSNKGKWEYDEKKIFNRIIVIIILSRVP
                     NLLHIVIDNRNVERILSQIIINVALYYLYNYIVSKNIKKTALELHDTNEELTKKTESL
                     KEKNKKLIYETEKIEELKGILMKRESRLQSTLDVAVNSIVVFSKDGEITYANKSFRNT
                     FGDYKEQDKLTDKIKNFNDLIESIHYVFVHEKNAEKLISTSDNKVYQAIFAPLIIASQ
                     TEGVLCVLEDKTKKKEYERKLIEANKRSKDFLESIGDGIVVLEGNNKIYVNNACREIF
                     KNNLESIDFSLVCKSEESIEKRYVIDGEVKYVEMSFSQYTNAGTNKTIIVIRDTTSRK
                     ISQIKLEESQSSYSRFIDILPDGICLLKEDLSINYANKSFLDMLAFTNIDDIKDSNIK
                     LLMNANTEEKMKFTDNMAKVLNENRSILLLEYELINSYDDIVEVELSALPFAIYNTRY
                     IMLIIKDLVHKKYSEQAEKELLERFKTDKIKTEFFANMSHELKTPLNVISSSNQLVDS
                     FYRNEKIKDYNNNIKSHVDLVRQSSYRLQRLINNIIDLTKMESGFYTLKLAKYNIVSV
                     IEDLFMNIEEYALRKDIKILFDTDLEEINVYIDKVEIERIMLNLLSNCIKFTDNGGWI
                     YVSIHYKIDKVIISVKDTGVGIPQDKLELIFEEFSQVDKTLSRNTEGSGIGLAIVKNL
                     VSLHGGDIEVVSEVNKGTEFLISLPMKGFSSEHYTEDKRIYNIQEKIKIEFSDIYY"
     gene            complement(28858..29802)
                     /gene="manA"
                     /locus_tag="ACOIAF_001096"
     CDS             complement(28858..29802)
                     /gene="manA"
                     /locus_tag="ACOIAF_001096"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HGZ2668117.1"
                     /translation="MEPLFLKPIFMDRIWGGTALKDKFNYEIDSPTTGECWAISSHKN
                     GDCLIENGKYKGKKLSELWNKNRELFGNTPGDKFPLLTKILDANDNLSVQVHPNDEYA
                     KKNENGELGKTECWYVIDCSDDAEIIIGHNAKSHKEFVNMVNNNEWDKLLRKVNIKKG
                     DFFYVPSGTIHAICKGTLILETQQNSDTTYRVYDYDRTDNSGNKRELHVQKSIDVTNV
                     PHINFDTDYKIVSTSDFKCTTFVSNEFFSVYKLDVFGKCNFTHNTPFSLYSVLDGNGK
                     LIHNSVEYNLKKGMHFILPNDFGDFSFEGNLEIICSHI"
     gene            29975..31336
                     /locus_tag="ACOIAF_001097"
     CDS             29975..31336
                     /locus_tag="ACOIAF_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HGZ2668118.1"
                     /translation="MELFKAESKKYKKEFLKLLEQWVSIPSFYDRKTVSKDMPFGKGV
                     YDALNWFENLGRENNFKVKNIDNHAVQIEYGNGKEYVDIFGHCDVVNPGEGWDSEPFK
                     LNIIGDKLVARGVSDNKGPMIVNFLALKMIKDLNINLKRKVRLIAGGNEESGFKCIKH
                     YYSKEPYGVCGFTPDAKFPVLNGEKGGAIIKLISNIDDKSLYISGGIEFNTIPDKVYI
                     KNVEKLGKDNICFDINNISINYDNGNYIVYGKGGHSSKPEKSINPILATIKLLSENID
                     EKWTKDLYKLINQDNINGNLFGLNIEGKCGILSMVPTIINIVDGKLEVVLSVRYPEIL
                     TLEDIIKKFNLYMEQNNINKFELIGENLKQANYIDRNSKLVRSLHDIYIKYSGDLKND
                     VRVTSAGSYASEMNNSVIFGCEFPDGSFGNVHSANEFASLDRFITAIGIYAEAIITLC
                     NKI"
     gene            31424..33313
                     /locus_tag="ACOIAF_001098"
     CDS             31424..33313
                     /locus_tag="ACOIAF_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HGZ2668119.1"
                     /translation="MITKKHAIIVKSLSNKDGYMTSNELAIKLDVSTKTIKRYIADLN
                     SVLSKYDLVIASSRGIGYKLSGSKNNIARAIKEANKYIDGFLDDSEESRMSNIICMLI
                     NRNYMSIEAMAEELNLSIAAINKLSSKLKRKLEKYDLVIKSKPYYGSYIHGEEINIRQ
                     LITDYAIKLDEKSKVKVFLDDISENDIHCIESILEKHLREKDTIISDKDFNLLLSKII
                     VSVFRSKRGHSNNINLMDTSYRFHNYYFIENLMKEISDKIGFKLIEDEVIYISNYSGV
                     IAYKGTQGRKNTSEIEERISSVISSALQDIFLISGSDYTKDDEFMVAISDHIKRFLNR
                     ARANVKSNNPLLHQIKEKFPIAFNLAVFLSNKLETEFNLKLDEDELGYIAIHFAASNE
                     RMKKNTSKKICIVCHYGIGTGQLISEKLKQNISDLSVVGVYPVRYLDMAISQDVDLIV
                     STVELKGYEKPVLYIENIFDDSLIENVNKAFYEKEERRKIISNMFDEKAFFSIKANTK
                     EEVIISLSNKLKERDFIEESSIKSIIDRENISSTEIGNLVAIPHTIVKGDKKSIIGVG
                     ILENPIIWDKQEVQLVFMVFFNTKEKHNFSIFKYLYNFIKDEGGVRGIIKICDFNKLM
                     ALIGN"
     gene            33328..33783
                     /locus_tag="ACOIAF_001099"
     CDS             33328..33783
                     /locus_tag="ACOIAF_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGZ2668120.1"
                     /translation="MGTNIFNKEYVFLNVDAKSKVEVLKFISKKAKDLNLAEDESLVY
                     EGLMAREEQFTTNLGESIAIPHTKNDAIENPAVVVLKFNEDVVWNEGEDKVKLAISLL
                     MPGKSKENIHLKLLSSLSRKLINKEFKDSLLKSDNVEEISNLINEALGL"
     gene            33842..34159
                     /locus_tag="ACOIAF_001100"
     CDS             33842..34159
                     /locus_tag="ACOIAF_001100"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: protein motif:HMM:NF014368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HGZ2668121.1"
                     /translation="MNKKLVALCACPMGLAHTFMAAEAIEQAAKALGYEAKVETQGAD
                     GVQNELTRDDILGATMIIHAVAITPEGMERFDGCEVYEVELQEAIKNAEGVIKEIEED
                     LGI"
     gene            34184..35446
                     /locus_tag="ACOIAF_001101"
     CDS             34184..35446
                     /locus_tag="ACOIAF_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HGZ2668122.1"
                     /translation="MAIKKKVINSSGASTTGGNNVGKSTPSSPTQSKGNGKWKEISKH
                     IMTGISYMIPVLVMGGLIGALSQLIPYAILGLDPSVGIVDAMNSGEFTGFKLSLLNIA
                     QLMSNFGFTLFGFAIPLFAAFCANSIGGKTALIAGFIGGYIANKPVGVPQFVDGQWTE
                     VVPVASGFLGAILIAFIIGYFVKWLNKSIKVSHNWLAFKTTFLIPLIASLACMVLMIF
                     IITPFGGLINESMKNFLTAAGAAGEYVYATALAAATAFDLGGPINKAAGFVALGLTTE
                     NVLPITARTIAIVVPPIGLGLTTLLDKRLVGRRVYDRQFYQAGKTSIFLSFMGISEGA
                     IPFALERPGFVIPLNIVGSVIGAITGIILGAIQWFPESAIWAWPLVDNLFGYIIGIAV
                     GAIFIAVGNIFYRNKLIKDGKLVVDYID"
     gene            35551..36615
                     /locus_tag="ACOIAF_001102"
     CDS             35551..36615
                     /locus_tag="ACOIAF_001102"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HGZ2668123.1"
                     /translation="MDKIEKVREYFREYDIDGFLINSPTNKFYVGNLFSSSGYVFITK
                     ESQYIIVDFRYFEEIKRKSSLFNVVLMDKTRTHFDIINDICREQNIKEIGFEGNEVSF
                     DLYRSMSNKLSATLKSVDLSTLRETKNEDEIKYIKKACEIVDATFYHIVDFIKVGMTE
                     KQVENEIVRIIKDLGGQKESFDTIVASGLRGALPHGKASEKVIEYGDFVTFDFGAKYN
                     NYCSDITRTICMGTINKELEEIYNIVRKANEECIRVLRPGMTTGEIDKVARDIIGSYG
                     YAGNFGHNLGHGVGIMVHEYPALAPESNEVLKEGMIVTIEPGIYVPSLGGVRIEDDVL
                     ITQDGCMRLTTSTKDLIVVS"
     gene            36669..37847
                     /locus_tag="ACOIAF_001103"
     CDS             36669..37847
                     /locus_tag="ACOIAF_001103"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HGZ2668124.1"
                     /translation="MYNFDDKPDRVSEKCRKWDLNIIRDKFGDIREDFVPMWIADMDF
                     KIPTEIENKFIEAVRRGVFGYTYCYDEFYDSVIGWQKNMHEVNVEKEWITLTYGTVST
                     LHYVVQAFCKKGDSIILNTPVYDPFESSAKKQGVNVICNTLDVVNDRYYINFDKLEVQ
                     IKENKPKLMMFCTPHNPSGRIWTIEEMTRVATICKENNVILVADEVHAEHIHYGKFNS
                     ILKIGKELLENVILLTSPNKGFNLGGLKTSYSIVINKDIRDKFRNKLKQNSITSPNVF
                     GIIGLITAYNECHEWLSGVNEYIKSNYELLETWVNKYNKIKLMKMESSYLAWMDISGL
                     GISASEFTDKLAIDTGVLLEDGSHFVKDGEKYVRINLGTQKENVIEALNRMDLFLKSL
                     "
     gene            complement(38038..39096)
                     /locus_tag="ACOIAF_001104"
     CDS             complement(38038..39096)
                     /locus_tag="ACOIAF_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HGZ2668125.1"
                     /translation="MNKKKITFIFVILILALILFSFFRSSSQNTKYSKTSYYLGTVNE
                     VTVFNVKESKSDKILNECDSILRYIDNKMSTHIPGSDVSKINDNAGKKFVRVSDDTFF
                     VVKEAIQYSKLSDGYFDITIGPLSNLWAIGTDKAKVPSDSEIQKLLPLIDYKNIILDE
                     KNKSIKLTKENMKIDLGAIAKGYAADKIVAYLKSEDVKKGLVNLGGNIFTLENGKNDK
                     PFKIGIQDPTSKNGESIGNIETTNKSVVTSGIYERFIEKNGKIYHHMLNPFTGYPFEN
                     NLSSVTIISDKSINCDALSTSTFGLGLEKGMDLINKIDNVDAIFITKDKKVYLTKGIK
                     DNFKLTDKSFSIEKLNKQ"
     gene            39163..39534
                     /locus_tag="ACOIAF_001105"
     CDS             39163..39534
                     /locus_tag="ACOIAF_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HGZ2668126.1"
                     /translation="MKKKDILLILGILVVITACYGIINVINSKNAGNIEIYVDNKLYK
                     TVSINAKEEFKIENRGGYNIVKIHDKGVEIVDASCPDKVCVHTGFINKPSQSIVCIPN
                     RVSIKINTNEKNDNQEDIISK"
     gene            39571..40245
                     /locus_tag="ACOIAF_001106"
     CDS             39571..40245
                     /locus_tag="ACOIAF_001106"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HGZ2668127.1"
                     /translation="MVNLGNDWDELLKEEFEKDYYLNLRKFLIEEYKTRQIFPNMHNI
                     YEALKHTSYKDTKVLILGQDPYHGDNQAHGLAFSVQPQVKTPPSLLNMYKELKDDLGC
                     FIPNNGYLMPWADQGVLLLNTALTVRAHEANSHKNKGWEIFTDRVISILSEREDPVIF
                     VLWGSNARKKVELIDTSKHYILEAPHPSPLSASKGFFGCKHFSKINEILKKLGKEPIN
                     WQIENI"
     gene            40494..41333
                     /locus_tag="ACOIAF_001107"
     CDS             40494..41333
                     /locus_tag="ACOIAF_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HGZ2668128.1"
                     /translation="MNFKKLSNAKVSVMAVVLSVGILSGYSALNKQVTLVVRGEERTI
                     STFSSNVGDLLESQNISYDANDIVTLDENTKLSNGDKIEVIDVKEKTVVETKDIPFEV
                     TVVEDGSLLKGDSKVKEEGKSGKSELIYKETYHNGKKVEKEFVKKVVKAEPVNKVVSK
                     GTKVEMQVASSRGESSRRMASSSSSSSSSNNGKNMRVVATAYTGHSITATGTKPKWGT
                     IAVDPKVIPYGTKVYIPQFDMVFTAEDCGGAIKGNKIDIYMNDSKTVYNWGRKTIDIR
                     ILK"
     gene            41577..41673
                     /locus_tag="ACOIAF_001108"
     tRNA            41577..41673
                     /locus_tag="ACOIAF_001108"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:41611..41613,aa:Sec,seq:tca)
     gene            41824..42531
                     /locus_tag="ACOIAF_001109"
     CDS             41824..42531
                     /locus_tag="ACOIAF_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HGZ2668129.1"
                     /translation="MENNFTTRTSFLVGDDGIKKLNNSNIIVFGVGGVGSFTVEALAR
                     AGVGNITIVDFDDVDITNINRQIPALHSTVGRYKVDVMEERILDINPNINIKKIRSLY
                     NKDTSDEILTERYDYVVDAIDMVSSKIHLIETCEKKGLKIISSMGMGNKLDPTKIVVT
                     DIHKTSTCPLAKVMRKELRDRGIKKLKVVYSTEQPIELKKKVMNGRKVTPGSVSFVPS
                     VGGLIIASVIVNELLGQ"
     gene            complement(42673..43509)
                     /locus_tag="ACOIAF_001110"
     CDS             complement(42673..43509)
                     /locus_tag="ACOIAF_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HGZ2668130.1"
                     /translation="MRNKIFLSILIIISLLIGCDKKSLLSIHMIDVGQGDSILVQTPT
                     NKNILIDGGDEDSENIIISYLRQKRIKTIDIIIATHPDSDHIGSLDNIIKKFNVNSIY
                     MPEQSTDSEAYQNLINSCTDKNLSIQHLYKNDVLNIDNNINIYVLSPSYIQEESNLNS
                     IVFKLTFNDNSFLFMGDAEEENEKEILHSFKLNNINFIKIGHHGSNSSSSLEFIKKIS
                     PDIAAISCGYKNQYGHPHREVINNLKQNHVSIYRTDRIGDIVFYSDGEIIFTKYNYEI
                     DT"
BASE COUNT        17024 a         4808 c         7524 g        14189 t
ORIGIN      
        1 atatttttaa attatcattt aatgtaattt aaatgaattg aagatatatg tttattgtat
       61 gcaattgttg tatttttagg ttttttgtga ttaattaaaa aattataaaa atcgtttaca
      121 tgttgatttt taaaaaaaaa agatatagaa ttatatagaa agtaataaac aaaaattgga
      181 tttacgggag tggaatgaaa tgatgaatgt taccttaaca gatgtaaaag aggctagaga
      241 gaccattaag aacatagtaa aaagaactga cttacttgaa agtgttaaac ttagcgaaaa
      301 aacaggagca aatgtgttct ataaatgtga gaatcttcaa aaaacaggtt cttttaaatt
      361 aagaggagct tgtaacaaaa tagcgagttt aacagatgaa gaaaaggcaa atggagttat
      421 agcttcaagt gcagggaatc atgctcaagg agttgcattg ggagcaaaaa tgactggtat
      481 aaaatctaca atagtaatgc cagcaactgc gcctttagca aaagttagtg caactaaagg
      541 ttatggagca gaagtagttt taaatggtgc ggtttatgat gatgcatatg caaaagctgt
      601 tgaaatacaa aaagaaacag gagcaacttt cttacatcca ttcaatgata agtatgttat
      661 agcaggacaa ggaactatat ctcttgaaat tttcgaacaa ttagacaata aagttgatac
      721 tatactatgc ccagttggtg gaggaggaat catctctggt gttgctgttg cagcaaaagc
      781 attaaatcct aatgtaaaaa tagtcggtgt tcaaactgca aatatacctt caatgaaaga
      841 gtcaataaaa aatggaaaag taacaacagc atttaatgat actacaatag ctgatggtat
      901 agctgttaaa actccaggag atttaacttt tgaaataata aatgaattgg ttgatgaaat
      961 agtagtggtt gaagagacag aaattgcaga atcaatatta tttatgatgg aaagtcaaaa
     1021 aatagtttca gaaggtgctg gtgctgtatg tacagctgct atattaagtg gtaagtatgt
     1081 accagcaaaa gatgaaaatg tagtttgtat aatatctggt ggtaatatag acataaatac
     1141 gttatataga ataataggtg ttgctttagc taaagaagga agaagatatt catttagtac
     1201 aattatggaa gataagccag gaaactttgc tgagttaact agaataataa gtgagaatgg
     1261 tggaaacatt ttaagtgcta atcaaggtaa attatcagca ggggaagctc taggaaaaca
     1321 aagtgcggaa tttatattag agacaataga ttatgaccat atagctagaa taaagaaagc
     1381 tatagaagaa aaaggtttta aaattataga attataataa aaagtgtata taatatacaa
     1441 gaaaaatagt ataatagtgt atgaagatac attttatgtg tattagatta acgaattagg
     1501 aggattttaa aatgaaacat gaagtaatac atactaatga tgcaccagca gctttaggac
     1561 catattctca agctataaaa gctggaaact tgttatttgt atctggacaa gttcctcttg
     1621 taccagagac tatggaagta gttgaaggag atgtacaagc tcaaactgct caatctttaa
     1681 aaaatcttaa agctatatta gctgaaagtg gagctgattt ttcaaatgta gttaagacta
     1741 cagtatttat aaaagatatg aatgaatttg gggctataaa cgaagtttat gctgaatatt
     1801 ttggagaaaa taaaccagca agagcttgtg tagaagttgc tagattacca aaagacgtta
     1861 aagttgaaat agaagtaata gctgtattat aaaaatacaa tatttttaat ttgaaaaggc
     1921 tgaaaaacta tattctagtg attcagcctt tttataaaaa aataaatcac aataaagaac
     1981 atgataaata ttgtcgaatt aaaaaataaa attaaaataa atattgcaat atttttgaga
     2041 aggttttata atgagtatat aatttgaaac gtgtaactat taaattaggc atgagttctt
     2101 ggatattttt atcctgagga ttcatgcctt ttttgtattg aaattcaaaa atctatttaa
     2161 taaggaggaa gtactgtgtt aaattttttc aagaaaaata aaagctataa gttacatgca
     2221 gtagtgagtg gtaacagcat taatattgag aaagtaaacg attctgtgtt ttcaaaaaag
     2281 ttaatgggag atggagttgc aattattcct aatagtgatg ttgttgtagc cccttgtaat
     2341 ggtaaagtaa ctgtgcttac agaatcaaag catgcctttg gtatggtctc agatgagggt
     2401 gttgaaatat tagtccacat aggaattgat acagttagtt tacagggtga ggggtttaaa
     2461 aatgaagtgt ctcaaggaga tactgttaaa aaaggtagtc caataataag ttttgaaaga
     2521 gaaaaaataa attctcaggg aattgattgt acaacaataa taattgtatt aaatcatagc
     2581 gaattttcag aaataaattg catggtagaa aatgaagttg ttgcagggca agatactgtt
     2641 attgaaatta tgaaataata ggaagggatt aattcaatat gagagtagta aaaatcttta
     2701 ataataatgc attatctaca gtaactaatg ataggaaaga agctatttta ctaggtcttg
     2761 gtataggttt taataaacgt ccaggagata aagttaatga agataaaata gaaaagattt
     2821 attatgttca agaccacatg caaacaaaat ttttagaatt attaaaaaat gttagtccag
     2881 aagtgatgga tgcttcacaa caaattataa gtcttatacc aggaaatgag ggaaaattta
     2941 ataataaagg gatattatct ttagtggaac atattagttt tgcaatagat agaatgaaaa
     3001 ataatgtttt tttacctaat ttaatgcttt tggatataaa gatgatgtat tcaaaagagt
     3061 ttgaattagg agtaaaagct ttagaaatta tatatagagt atgtcatatc catttgcctg
     3121 aagatgaagc aggttacata gctttacatt ttgtcaattt acaatcaaat gataatttgg
     3181 catatgatac tttaaaattt gtaaaaggta gtattgattt gattaaagaa tgttatggat
     3241 tagaattaga cgaatcgagt ttaagtacat tacgttttag aactcatctt aaatttcttg
     3301 ctcaaagaat ttttaagaat gaaatttgtc aggatgataa aatgattgaa atgtatgatt
     3361 atttaattaa caatcatcct aaaaataaag aatatttaga aaagttaaat gtatatattg
     3421 aaaaggagtt taggtataaa ttagataaac ctgaaaaaat ttatctacta ctacacctaa
     3481 caaaaatctt ataagaatga gatgaattag taatagaaat atatataatt ttaggggggg
     3541 ataagattga aagaaaaact acttggagga ctggaaacat ttgcaaaggc aatggttcag
     3601 ccattaatgt atttatcgag tgcaggaata ttaatggtgg taggtgtact acttacaaat
     3661 gatatgttat taggagtact tccatttttt aaatgggagc caattcaaat atttggaagc
     3721 ctaatttata actgtataat ggctattatt aataatcttg ctgttatatt tgctataggt
     3781 attcctgctg cactagcaaa aaaagagaag cacaaagcag cgttaatagg atttatgagt
     3841 tatataatat accttacagc ttccaacact atgttaacat cactaggtca actagcaaaa
     3901 gaaagcaaga tgcttggact gatagggact ggacaagcta ctattttagg tattcaagtt
     3961 gtagatacaa gtgtatttgg tggaattatt ttagggtata tagctggata tgtatttaat
     4021 cgtacaagca acaaaacatt taaaggagct ttacagatat actcgggagc aaattttagt
     4081 tttatatgca tgttctttgt atccatttta attggagttg tgagtaactt tatatggcca
     4141 ttagctcaac aaggaatagc ttctcttgca aatgtcatca aagatacagg tacatttggt
     4201 ttattcttat atggtttttt agagagaata ttagtaccaa ctggacttca tcatttaatt
     4261 tatacaccat ttcagttttc tgatttagga ggagtgctta aagttggtgc agatacagtt
     4321 tcaggagcct atgctatagt tatgacagaa atgaatttac cagttgagcg tttctcagat
     4381 agtatatact acatggcaac tggatttaca aagatgtttg gttatatagg tataggagct
     4441 gcctttattt atactgcata taagcaaaat aagaagaagg ttaaggcaac gattattcca
     4501 ctaataatta cggcagtatt agcctctgta acagaaccat tagactttat gtttgtattc
     4561 tgtgcaccac ttttattctt tgtacatgca gttatagctg gtctttttat agctttatta
     4621 aaaatatttg atgttactgc attatgtgga gggaacttat tagtatcagt tataatgaat
     4681 actacagtag gcgtagaaaa aacacactgg ccagtaatga tgatattagg acttgcacaa
     4741 attattgtat attttgtagt atttagtttt ttaattaaga aatttaatta taaaactcca
     4801 ggtcgtgaag atgagagtac agctacaggc gaagaacaat caaaacaact taatttagat
     4861 gcagggatag aaaatataat tgatgggtta ggcggaaaag aaaatattaa tacagtagaa
     4921 aattgcatca ctagattacg tgttaatgta aaagatgaaa gtaagattaa tgaagatata
     4981 attaatttaa cgccaaatag cgggattgta aggaaaggaa aagatataca aataattttt
     5041 ggacttcatg tgcatgaggt aagacgtgca gtagaagatt ttttagaagc ttattaaaga
     5101 tatatattta tatagcaagg gtaaataaca gctttaagtg ctgtttacta tttattaaag
     5161 atacatataa tttatagagg aggaataatt attatgatta ttactattgt tggtggagga
     5221 agcacattta ctccaggtat tgttaagtca attgcattaa gagaaaaaga gttagagctt
     5281 gaggaaattc gtttatttga tattgataaa aaacgtcaag ataaagtagc tgtagttgtc
     5341 aactggatac taaaagaaga tgttaaaaca gatgttaaac taactgtaac taattgtgaa
     5401 aaagaagcgt atacagatgc tacatttata tttgcacaaa tgcgtgttgg taaatatgaa
     5461 atgcgtgagc aggatgaaaa aattccattg aaatatggtt gtgtaggtca agaaacttgt
     5521 ggttgtggag ggatggcata cggactgcgt acaatacaac caatgataaa ggttattgat
     5581 gatgtagaga aatatgctaa aaaagattat tggatattaa attactctaa tcctgctgca
     5641 atcgtttctg aagcttgtcg taagttaaga ccaaatgcaa ggattataaa tatctgtgat
     5701 atgccaatag ctattataga tatgatagcg gggtcattaa atatcaatga cgttcataat
     5761 atacgctatg attattttgg tctaaatcat tttggctggt ttacttcaat agattataaa
     5821 catagagact taatggaaga gattaaggaa tacataaaag aaaataaaat attattacca
     5881 aaatcttata tagaaaactt aaataaaagt cgtagtacag gaaatcgcca tgctgtaagt
     5941 agctggatac atgtttggga atctgtttat acaatgttag aaagcttccc aaaatatctg
     6001 ccaaacactt atttaacata ctatctttta agtaaagaaa caatggaaca ttcaaacata
     6061 aatcgtactc gtgcaaatga ggttatggaa agccgtgaaa aagacttatt tgaaggtgtg
     6121 agacattact tagaaactgg agaaatttct gaaaaagcat tttatgcagg ttcacatgga
     6181 gattggatta gtgatttggc agtttcaatt aaaaatgata cacgtagtcg ttttttagtt
     6241 ataactgaaa atcgtggagc tataccaaac atgccatatg atgcaatggt agaaattcca
     6301 gcatatattg gtaaagatgg tccagaagtt attgctcgag gtccaatacc tctattccaa
     6361 caaggattaa tgatgcaaca acttaacagt gaaaaattga tagtagaagc ttatatagaa
     6421 aatagttatg aaaaagcttt acaagcattc acattaaata aaactattcc aagtatgaat
     6481 gttgctaaag aaattttgga tgaaatgata gaagttaata aggaatactg gccagaatta
     6541 aaatagtagg ataaaagaaa gggtttttat ttaataatta gatatgtttt cagagtcttt
     6601 tatcaaaatg tattcacaaa catatggcaa tatattaaaa taaagagtga tatttttgga
     6661 agaatggggt gatgcaatga acattataaa taaaattttt tggcaagaag ataggattgg
     6721 catgattaca aataatttag aggcagatac acatagccac tgtatgttac aactgttctt
     6781 agggatagaa gacagcattg aaataacagt caatgaaaaa ttagttaaat gtaattgtat
     6841 catagtagat aaaaatatct ctcattcatt ttcagcaaga aaaaaagttt attactctgc
     6901 tattattgaa cctacatcta tatatgcaga acagcttacc tcaaaaatga atgattttgg
     6961 ctactggata tgtgacaatg atggattaga gaaattacgt caacaaggaa cttttttgat
     7021 agataactct agtaaagaac aatatttaag atttatggaa atgcttaata actatttgaa
     7081 tatacctata actctcaaac attatgatga cagaattact gaattgctca atttattacg
     7141 tacttgtaat tgtgataatc atactatttc aagttttgca gataaagtgt ccctatcagc
     7201 aagtagatta tcacatctgt ttaaagaaca aatagggata cctttaaaaa gctatatctt
     7261 atttcatcaa atggagtgtg cttttagaga attactttct ggaaaaaatg ttaccgaagc
     7321 ttctatgact gctggattcg atactccatc acactttgca gggacagtta agcgaatgat
     7381 gggaatgcct gtttctttat ctttgaaaga tagcgagttt ttgaaagtat attaaatatt
     7441 ctcgttatat aatgaagttg tactaagaaa ggggctttgt tatgaataaa gaatatttag
     7501 aagaaacaaa aatgttaaat tataatgaac cacaacttaa gcttttggta tcatcaaaga
     7561 attggttgga attagatgat tttcataaaa taaaatctat ttatgagttt gtacagaatg
     7621 atattttatt tggatataat gcttctgata tgttaagcgc aacacaggta ttaaatgatg
     7681 gtatgggaca atgcaataca aaagctactc ttttaatggc attattacgt gcagtgaata
     7741 tcccttgtag gcttcatgct tttgatgtaa caaaagattt tcaacgagga gctacatcca
     7801 aattgatttc actattagca ccaaaatata ttcttcatac ttgggtagag gtattttatc
     7861 aagataggtg gattgctctg gagggtgtca taactgataa aaaatattta gaagcaattc
     7921 agaagaaatt ttttaatcat ggtggaacgt ttaaaaagta tgcaatagct acaaatgatt
     7981 tgaaaaatac atctattgat tgggatggta aagatacttt tatccaaaaa gaagctatcg
     8041 tgtatgatta tggtattttt ccatcaccag acgtattttt ttctactcat tcacaacata
     8101 tgtcaaaatt aaaaaatttc atatatgtac atcttatcag aaaaataatg acaaaaaatg
     8161 tatgtaaggc tagaaacaat tatattgata aaaatgaatg agtgtttaag aggtaactat
     8221 ataggaaata tatagaataa ttaaaactgt actggttaaa atgaataaaa aggagttatc
     8281 acagagcaaa cttaaattgt tcataggata actccttttc tgtattaact tctaataatt
     8341 tgatgtcttt ttataaaaca tatagataca attaaaccag taaatgcaag agtagctgct
     8401 attgaaaatg caacatctat tccatgaatg ttagacagca tgtttgaact catatgagct
     8461 ttcttagtag cacttgtcat tatagtaata agtatagcag aacctatagc tccagatatc
     8521 tgacggagtg tgttattaat tgctgtggca tgagatataa gttctctatc taatgtttta
     8581 attccccatg tagttaatgg cattaaaacc attgaaatac caatcatacg aatacaatac
     8641 atcaatgaca catgaattaa agatgtatct ctgcctaaaa aagcgaagga taaagttcct
     8701 aaaaacaaac atcctgttcc taatattgat agagcatgag gtccatattt atctaaatga
     8761 cgtcctgcaa ttggattgag tacaaccatc aatatagcac caggtaacat taatgaacct
     8821 gaagataatg cagaaaatcc tcttacagat tgaacgtaca gaggaatcat taatgtagca
     8881 gacatcattg aggcatatac aatcataatt aatattgtag aaataagaaa tggtttattt
     8941 ttaaaaaccc tcaattctaa aaaaggttct ttagatttta attgacgcaa tgtaaataaa
     9001 attaatgaca taattcctat taatattggt aaatatgtgg caatattagt ccacccataa
     9061 ttaccttgat tagtaaatcc aattaataaa ccaccaaaac ctagagatga tagtactatt
     9121 gaaggtatat ctaattttag tttttgagtt tccccaacat ttcttaataa aataaatgat
     9181 agtataacat ctaatatagc aataggggaa agaaacagaa ataaactatg ccaaccaaat
     9241 gaatctacta accatcctga taaagttgga cctattgctg gagcaaatcc tactgtaata
     9301 ccaactaaac tcatagctgc accacgtttt tcctcagggc ataaatagag tataataact
     9361 tgtaaaagtg gcattagaga accagaaccc attgcctgca acacacgagc aatcaacata
     9421 gttgaaaaat tattagaaaa aagtgcaata atacatccta tagaaaagat agacattgat
     9481 gtaataaata actttctagt actaaatcga tttattaaat aagctgttgt gggtacaata
     9541 atacctgcaa taagtagata aattgttgtt aaccactgac caacatcagc agtaatttta
     9601 aaatctgaca ttatattagg taatgctgtt gacaatagtg tttgactgag agaacttata
     9661 aaagcaccag tcattacaat tgctattatt aaatttcctt ttttattgtt actttgggtt
     9721 tccataattt ataacgtctc cttttttaat tgatttttaa tgctgttcaa taaaagtact
     9781 tttggtctta aagtgtcttc tttttctaaa tatgcaatca ttgcatccat aattaaagaa
     9841 tcatattgtt gattttttcc ctcgacgtat aattttttta ttgcttctag taatttagga
     9901 ttttcaagga gagaagtatc agttttgatt agttcttcta tatcttgtat cgttttaaaa
     9961 aatagcgatt tattatgaag ggaaaagtaa acttcatctt cttgtttatg taaaaacatt
    10021 gatttttcaa ttaaaggatt tagattatcc atattttcta caactaaatc tagaatttgt
    10081 atagtaaatt tggcaataaa gtatacattt atttctcttt tttgtacatg aataatgtat
    10141 ataaactgat gaattagagc atccaataag aaaactaaat caaatataat tttttctatt
    10201 ttatcaccat agttatcaaa taagcttaat ttgtattcat tcaataagtt agctcgtgat
    10261 tttgtttgca atttacttgt agccttacgt tgctcttcag aaaaattttc tcttatatag
    10321 gtatttatgg tagttctaga aaaaatattt ttccatacag caattatctt tctgtttaac
    10381 ttttctgatg ctggaatgtc attattttca tttatggaat gtataatttc taaaaacttt
    10441 tcatcataat aattgattat ttcgcagata aaactttcct tagtttcaaa atgcttgtaa
    10501 aaagtagctt tagacatttt acactctttt acaacatccg atattgaagt attttgaaaa
    10561 cccttttggt caaaaatttt ttgtgcaata gttataatga gcctttgttt ttctgtcact
    10621 ttattatctc ctttcacgca aaaacgttta gtactataga gtacctacaa gttttaataa
    10681 atatattata catcatttgg aaaaaaaatc aatatataga ctgtaatatg aaatttttga
    10741 aaaatttgtt gttattttta gtcgtataaa aaagaaactt aagagtttct taagaattgt
    10801 ttaaagaagt tgcaataaat tagtggtatg attatagtat gaaagagaat gtcttgaaat
    10861 ttttctgtat caaattccaa ttatggaggt gtagtatatg aaaaagaaaa tttgtgtagc
    10921 aataattttt gctctattta tagtttttgt agaaatgttt attgatagct ctagcgaagg
    10981 aagtaaaatt atgacagctg aattaagtaa atacaggaaa ggcgtaaatg tattatcaat
    11041 aaacaatatt atagtagcga ataaaaaata tagtttgccc aaagattatt ctcctcagga
    11101 aagcagtgaa gcgagagatg ctttttataa aatgaataaa gatgctcaaa agtcaggatt
    11161 aaatttaaaa gcttttagta catatagaag ttatgaatat caagatagat tatttaagtc
    11221 atatgtaaaa gaacatggag aaaaagaagc aaatagattt tcagctaaac ctggagagag
    11281 tgagcatcaa actggacttg cttttgatat tggaggagat gaccagtctt gttgggcaaa
    11341 taaaaagttc aacaatacaa aagaagctaa gtggttgtat gaaaatgcat ataaatatgg
    11401 ttttatactt agatatccag aaggtaaaga gaatataaca ggatatatgt atgaatcttg
    11461 gcactataga tatgttggaa ctgaacatag caaaaacttt gcaatgaata atttgacttt
    11521 agaagaatat ttacatataa attaagagat ggtttgttaa aattaggtta aaataaaaaa
    11581 taacctagac tattagctgt gtaaagtgat ataatgtaaa tacaatttag ataactactc
    11641 gaaggggagt agcttacacg aaagtggtaa ttaatcgtca agacagtaga aatactcggt
    11701 taattagcaa gggattcctt gatagcaaga ccttcaaaat aagcattagg tatgtttgtt
    11761 ttggggtctt tttttattca aggaattttt atttaaaaaa atttaaattt caagtataca
    11821 taagagcccc caaacataaa ataaatttat tgaagaggag ggcaacaaat gtcttacaaa
    11881 agtacttatc aagaagtaat taaaaattta gactcaagtt tatctggttt gagtaaagaa
    11941 agagcagaag agttgttaga aaaaaatgga gctaatgaat taaaagaagc agataaggtt
    12001 cctacttata agcttttcct agaaagtttt aaagacccat tagtaataat tctattgata
    12061 gctgctttag tacaaatatt tttaggtgaa acagtggagt ctataataat ttttgcagtt
    12121 attattataa actctgttct gggtgttgta caaacaaaaa aagcagaaag ttcattagaa
    12181 agtttgaaaa atttatctgc accaaacgca aaggtaataa gagacaataa aaaaatgaca
    12241 attccagcaa aaaatctagt agttggagat attgtattct tagaagctgg cgattacata
    12301 ccagcagatg gaaggcttat agaagcacaa tctctcaagg ttgtagaagg gatgttaact
    12361 ggagaatcag aaccagtttt aaagcataca gaaaaaataa gtgaagatgt aagtttaggt
    12421 gaccagaaaa atatggtatt tagtggtgct gtagttgttt atggtagagg ttcttttgta
    12481 gtgactggaa caggtatgga tactgagatg ggtaaaatag ctgggctttt agaaagtgct
    12541 gaaaataaac ttacaccatt gcaacaaaaa atagaggatt ttagtaaaaa acttggtgta
    12601 ggtattgtaa tacttgctct tttaatattt ataattcaag tagcaagaaa ttactttatg
    12661 gctggaaatg gagaaatgac acaaattatt ttagattcat tcatgttctc tgtagcagta
    12721 gcagtagcag ctataccaga agctctatca tcaatagtca ctatagtatt ggctgttgga
    12781 acaaatagta tggcaaaaaa acaagccata ataagaaaat taccagcagt agaaacttta
    12841 ggttcaacaa gtgtaatatg tactgataaa acaggtactc ttacacaaaa taaaatgaca
    12901 gtagttgatt attatatgta tggaactaat aatgatgagt tagataaaga aaaagtaaac
    12961 tattcttatc atgctaaatt attgactatg gtttctacat tgtgtaatga ctctcatata
    13021 actagtgatg gtaaagaagt tggtgaccca acagaagtgg ctttaataaa ttattcaagt
    13081 aaaaatgatt tagattatga taaattaaga agtaagtata tcagaataaa tgaaatacca
    13141 tttgactcag acagaaagct tatgtctact gtaaatagta tatatggaga ttatataatg
    13201 tttacaaaag gtgctccaga tattgtgttt agtaggtgta aatatgcttt gaaaaatggt
    13261 tcaaaagtag atataacaga tgagataata gaagaatata aacataaaaa tgaagagttt
    13321 tcaaatagag cacttagagt attagctttt gcaataaaag atgtcccaga agaagatttt
    13381 ataccttcaa ttgatgacga acatgacatg acattggttg gaataatggc aatgatagac
    13441 ccaccaagag aagaagtaat ggatgcagta aaagaggcaa agagtgctgg tataaaaact
    13501 gttatgataa ctggtgacca taaaacaaca gcagcagcaa ttgcaaaaga gattggtata
    13561 atggaagaag gagacttggc tcttactgga aaagagcttg atgctttgag tgaagaacaa
    13621 ctatatgaaa aactagaaaa tataagtgta tatgccagag tctcgccaga aaataaaatt
    13681 cgtatagtaa aagcatggca acataagaat aatataacag caatgactgg tgatggtgtt
    13741 aatgatgcac ctgcattaaa acaagcagat ataggaatcg gtatgggaag tggtactgat
    13801 gtagcaaaag atgcttctgc tatggtattg gttgatgata actttgcaag tatagtaaat
    13861 gctgttgaag ttggtagaac agtatacagt aatataataa aatcaattac ttatctattt
    13921 gcaggaaact taggagctat agtatccata gtatttgcag tgtttgcagg ttggtcaaat
    13981 ccatttacag ctttacaact tttgtttata aacttagtaa atgactcttt accagcaatt
    14041 gcattagggt ttgaaaagtc agaaaaaaat ataatgaata aacctcctag acacccggat
    14101 gaaagtatac tagcaggagg aacattgaag gcagttgcta taagaggttg tataataggt
    14161 gtagttacaa taattgcaca atatatagga ttacaagtat ctccagaact tggagtagca
    14221 atggcatttt caacattgat attagctaga ataatgcaaa cattacctgc acgttctaat
    14281 aatcaaacag taataaaatt aggttttttc agcaataaat acgttatagg agcagtttta
    14341 gtctgtcttg ggctttactc tttaacacta ataccagtgc taagaggaat attctctata
    14401 ccaacaacgt ttggattgta ccagttgggg atttgcctag ggttagcatt aatttcatca
    14461 atattaatgg aacttaacaa attaataaat agaagtgaag taaaataatc aaaaaattaa
    14521 agggcatatt gattttaaca atatgccttt tataaatagt ctaatttatt ctatattatc
    14581 gattatttgg aatgctttta taacttcagg taattctttt tcttcaggca ttctaaatct
    14641 tatgaaattt ttatcaagtc cagtaaaatc acttccagat ataactctta tttttaattt
    14701 agcaaactct tgttgtaaat ctatgtcctt gtttttatgt tcaactgtca taattgatac
    14761 agtatcacta gtttcagcta tattaaggtt tttccatgga attaataatt gattttttat
    14821 atcttttgtt ttttctttta attcttctat aaattgaaca tcatctaata aatttgctgc
    14881 aatactacga gatatttcag acacttcgta aggagtagaa atctttttaa tagggctaac
    14941 aagttgttct ggaagaactg catatcctgc tcttagtcct gctaaaccga atccttttga
    15001 aaatgttttt aaagcaatta cattatcata attatttaga agcttgacag ctgaattttc
    15061 ttttggcatg tattcaccat aagcttcatc taccataact gctatatcat atttagctgc
    15121 ttcctttact atattttcaa ttgatgataa aggtataatt tgtcctgtag gattgtttgg
    15181 attgtctatg taaattactt tatattctgg atttattttt tcaataaatt ctttttcatt
    15241 aaacttgaaa ttatcctctt tctttaaaag aacataatca taagttgcac catgcatttt
    15301 tatatctgtc tcatattctg agaactgagg tgaatatcct aaaactttat ctcccttttc
    15361 aataaatagt ctatttaaaa ggtaaataac atgtatagaa ccatctgcca aacaaattct
    15421 gtcagtatct aatgctataa aatctttcca ataatcgact atattatctt ttaaagcaat
    15481 tgaatgagga tatcctctta tcatatcaaa ttttaaagca ttaaaagctt ctacagcttt
    15541 gtttgagtaa gacacggtat ttataccttc accacagtct atctcgaata catcttctga
    15601 atgtccagat tctattgcat aacttttttt caactgcatt tcaatagctt tgttaatttt
    15661 tagtttcata ctttatcact ccttatttag tcaataataa atgttataaa agttgctaga
    15721 tttaaaatat tatgaaaata tataatagta aaaaatgaat ataaatatta taatagctca
    15781 ctttatttaa tatgattata tatcatatat tgtaaaattt ctagcattat aagactataa
    15841 attgaaaata ttcagaaaaa tactacaaga aacgatttat tttgctctta aaataagtta
    15901 aatgaatgtt attaattttg taaattgaaa tagtttaagt ataaagttat gatataatct
    15961 aataagggta attctttaga ttatattaag gaaatatagg tatacaataa aaagttatgg
    16021 agttggatta tgggatataa attaattgta acagatatgg atggaacttt attaggcaat
    16081 aatcataaag ttacagatga aaataaaact gcactacaaa aagttataaa aagcggtatt
    16141 aatgtaactt tagcaacagg aagagccttc gattcagcta aatgcaatgt agattttctt
    16201 aaagaggaca tgccaataat agcttgtaat ggttcattaa taagagagca aaatggaaat
    16261 ataatatatt caaataagat tgatacaaga acatgtttaa acattttaga tgtattagat
    16321 aaatacgata tttactacca atgtaatagt atagattcta tgctttcgaa aaaaatagaa
    16381 ggacgtgaag atagattaag tgtattttta ggctctgaaa cagaagtaat tgtaaaagat
    16441 gatttgagag aagaaatctt taaaaaagat atactaaaat ttgtaatcat agaagaaaaa
    16501 aatccatcta ttttagatga aataagaaaa gaattgagaa aagttcaagg aataaagata
    16561 acttcttcat ggccaaacaa tattgaagtc atgaatgaag gtgttgataa aggtaatgca
    16621 gttaaaattt tagctgaaaa aatgaatata gacagagaag atataattgc atttggagac
    16681 aattacaatg acatagaaat gataaagttt gctggtcttg gggtagctat gggaaatgca
    16741 gaagaattga ttaaacaaga agctgattat gttacagata caaatcaaga cagtggagtt
    16801 gcaaaggcaa tttataaatt cctagagtta aaagaatcac ttagctctta aaacagtata
    16861 taataggaaa aaataggttg actattttgt agatagtaat tataatacaa atagataaaa
    16921 cataaaaatt tagattcagt gaaaaagaga gtagatatag agaaaatctc agcgaattgg
    16981 gggatggtgt gagcctaatg ataacacttt atattgaaaa gaactttgga gaatactact
    17041 tgaaattcag tatggtagta ggtaatctcg ccttaagaga atagagtggg taaacattgt
    17101 ttaccaacta gagtggtaac acggaatatt tcgtctcttg gtctttttga ccaagagatt
    17161 ttttagtttc attgagttac aagacttata attaaaatac aaaaaattaa aatatgaggt
    17221 gaaagttatg aaattatggg gcggacgctt tagaaaagcg gaaaatcaac ttatggaaga
    17281 atttaataaa tcttttggct atgattgtgt actgtacaaa aaagatatcg aaggaagtgt
    17341 agcacatgtt catatgcaag taaaatgtgg acttttaaca gaagaagaag gaaagtctat
    17401 aacagaaggt ttaaaaggga tacttgaaga tgtagaaaat ggaaagttag tattggatgg
    17461 agagtatgaa gatatacata gttttactga gataaattta attcaaagaa ttggagatgt
    17521 tggcaagaag cttcatacag cacgtagtag aaatgaccaa gttgcagttg atatgagatt
    17581 gtatgctaaa gaaaaagcaa atgatttggt gggtttaata tctgaattta aagctactat
    17641 aaaagatgtt gctgacaaaa acccagtgat gatgccgggc tatactcatc ttcaaagggc
    17701 acaagtagtg acatttaagc atcacttaat ggcatattat agtatgtttg atagagatga
    17761 gaaaagaata aaaaatgcta tagaaatatt agatgaatca cctttaggct gtggagcttt
    17821 agcaggaact acgcatgata tagatagaag tataacttgt gaacaattag gatttaaaaa
    17881 agtagtagat aattttatgg atggagtaag tgatagagat tatttactag agctaatgag
    17941 tgatttttca attataatga tgcacttaag tagattaagt gaagagttga tactttggag
    18001 ttctcaagaa tttggatttg ttgagataga tgatttatat acaactggaa gtagtataat
    18061 gcctcagaag aaaaatccag atggtgcaga gcttataaga ggtaagactg gtagagtata
    18121 tggaaactta tttggtcttt ttacagtaat gaaaggaata ccattagcat ataataaaga
    18181 catgcaagaa gataaagaag gttttttcga tagtgtacat actcttgaaa tgtgtataca
    18241 aataatggat agaatgatag ccacactaaa ggtaaatgag gataaaatga aacaagcagt
    18301 aaaaaatggt tttttaaatg caacagaagt tgcggattat ttggttaaaa ataatgttgc
    18361 ttttagagat gcacatggta tagtaggaag tatcgttata tattgtgaag ataataaaaa
    18421 agcaatagaa gatttaacat tagaagaatt acaaaagttt tcagatgctt ttaaggaaga
    18481 tatttatgat tttattgact atgagagtat attaaataag ggaataaaaa agaatttaaa
    18541 ataatgtatt tatttttaaa agctgtaaat ttgaatgtta tcatttaaat ttacagcttt
    18601 ttattgtgta agattttatt atataaaagt aagaaactta aaagtagtag tatttaaaat
    18661 aaatacaaat aaaaaattgt tattaaaatg ataattaata tcaataagta ttgacaaaat
    18721 tatttctaaa tactattata tataatataa ataataatga aaatcaatat caaataaatg
    18781 ggaggaaatc taatgaactt attagtgatt ggtggtcatg aaagaatgga aaaagattat
    18841 atggttatgg ccaagaaaaa aggttataag acaaaggtat atactaccat gtcatctaaa
    18901 ttaaataatt ctataggaag accagatgca gttgtcatac ttacatcaac tgtttcacat
    18961 aagatgtcta gaacggttga gtctcaagct aaaaaacaag atatattaat cgtaaggcat
    19021 aaaaatagta gtaaggtagc atttaatgaa tgtctagata tggttgatga atgtctggga
    19081 aattgttcaa aatgtaagct taaagacaaa caaaaacaaa aaaatatata ggtataaaaa
    19141 atacataaac ataaaattaa aagtactgag ataatagtta taattattat ctcagtactt
    19201 ttagatttat aaggataagt atagtataaa ttttttatta taatttccaa ctacttgtta
    19261 ctttttcata ttttttttac ttctagcttt ttttcttttt ttcttcttta tatttgaagc
    19321 tattataaat aaaactaaaa gcactagaat acctgcaata atcttaacaa aagtcattaa
    19381 aacattattt tcagtcaaaa atggaagact gctatcaaga ttattttttg caattaagtc
    19441 tatactgttt tctagtttac cattattata tacatttaaa gtaccaacct tagctccttt
    19501 ttttataggc aaatcaattt tatttaactc tgttttggta tcataattgc cttgagattc
    19561 atctttttct aagaatatcc tataactatt ttttggttca taaatcaatt catgttgttt
    19621 tgtaaacaat acttttttag tatctgtgta tttttcttta tcaacaatag tagcacactt
    19681 aaagttatca aatccataat caatcagaat acgtgagtct aaatataaat cagcattagt
    19741 tgaattaaat acagcaacga taactcttct gccatctttt acagcactcg ataaaagaca
    19801 tttaccagca gcatcagtat atccagtttt tataccatca actatatcat attttatatt
    19861 tataggttga cctttgtatg taatcttatc atgtgatgtc aaaaacttat tagtgtttac
    19921 aaagtatctc tcataaggat atgcttttgt agcttcaaat ttaagtgact tagtttttac
    19981 aatttgtcta aaagtatcat tattcatagc ttctctagct atgagagcca tatcatgagc
    20041 tgttgtgtag tgatttgggt ctggtaatcc atgaggatta ttaaaatgag tattagtagc
    20101 tccaattttt tttgcctcag agttcatgag gtcaataaat ttttgtacat tgccaccacc
    20161 aacatatcta gctaatacaa aagcagcatc attagcagaa tgaaccaaaa gagcatctag
    20221 taactgcttt acagtaaaag attccccatt ttttagatac atactagagc catctatttg
    20281 aggtaaatct ttaatttcta ttacttgatt taaatcttta acctccttta gaactaaaca
    20341 agctgtccat actttagttg ttgaagctgg ataaagctta gaatttccat ccttattata
    20401 tagtattttg ccagactcat agtccattaa aactgcatgt ttagcaacta tagctggttt
    20461 attatctgca aaagagatat tagagcttcc taaaaatgaa gtaaatatca ataaacagat
    20521 taataaaagt gataaatttc ttttcattgt gagcctccaa attttaaatt catcttttag
    20581 tatagcatat atttgtcaat aattaaaaga aaggagtgat taatatatga aaaaaaaggt
    20641 aaaatttatt tcgttttttg ttttatttat tttaataata tcatcaataa tcgtttataa
    20701 atcgcttatt ttcaaagatg atatttatgt tgtaagtaaa aaaaatttac ctaatgaaaa
    20761 aacacatgtt gtagagaatg aaattaaagg tgaagataat aatcacaata aagtaactaa
    20821 agacatagat aaaaattttc aaaataataa aataactata tacataagtg gagcagtcaa
    20881 tagacctgga attgtaacta tagagtcaga caagagactt tatgatgctg tagaattact
    20941 aggaggaacg acaaaagaag cagatttaaa tggagtcaat ctatcagtaa gattagaaga
    21001 tgaacaacat tacataatac ctaaaatagg tgaagcaaca tctgttacaa gtaatgatga
    21061 ttccaataaa ccaaatcaga agaatgagtc aagaggtgag cctaaaaata aatccaataa
    21121 aaatatatct aacgaatcaa aagtaaatat aaatgtagcg actatagaag agttggattc
    21181 tctacctggg gttggagaag ctacagccaa taaaatttta caacacagag aagaaaatgg
    21241 acagtttagt tcaattgagg aaataaaaaa tgtaaatggt attggagata aaaagtatga
    21301 aaatataaaa gatttaattt gtgtagacta aaatataata caaaacttac atatagatat
    21361 gttattataa atatgtatgt atatgtgtgt acatgtggaa atttagtaaa ggaggcactt
    21421 ttatgaaaaa agaacaaaaa aaacttacag agatgacaac agcaggtggt tgagccgcta
    21481 aaatagggcc agaggttctg gcatcagttt tatctcaatt gcctaaaaat gataatatag
    21541 aaaatttatt agtaggacta gatacagcag atgatgctgc agtttataaa ttaaatgatg
    21601 atatggcact tatccaaact ttagacttct ttacaccaat ggtagatgac ccatacgtat
    21661 ttggtcaaat tgctgcatca aattcactat cagatgtata tgctatggga ggcaaacctt
    21721 tggttgctat gaatattgtt tgttttccat catgccatga tatggatgta cttgcagaaa
    21781 tattaaaagg tgggtttgat aaagtgaaag aaagtggagc acttttggtt ggagggcata
    21841 ctgtagatga caaagagcct aaatatggtc tttcagtttc tgggattgta agtccaaata
    21901 aagttttatc aaatgctact gcaaaacctg gtgataagtt aattataaca aaaccaattg
    21961 gagtgggtgt attaaataca gccatgaaag aaggtatggt agaacaacat atagcagata
    22021 aagtaataga aataatgata catttaaata aatatgcagc aatgagtttt gagaaatttg
    22081 atgtaaattc ggttacagat ataactggtt ttggtctact tggtcatact ttagaaatgg
    22141 ccaaagcttc tgaagtaagt attgaaattg aatcaaaaca tgtaccaatt attgaaggtg
    22201 caatagaaat ggcacaaatg ggaattatac ctgctggtat gtataaaaat atgcattatg
    22261 tatctaaaga tgtagaagtt gtaggaaata tagaagttgc agttcaagat attttatacg
    22321 acccacaaac atcaggaggg ttgttaattt cagtaaaaga agaacttgca gaggaacttg
    22381 taaaagatat gaaattaaat ggagcaatag aagctaaaat tataggttct gtagttccaa
    22441 aaggggaaaa atatattaca gttttataaa atattataga gaaagagtga ttgttttgag
    22501 caaaagagaa ttgtttgcaa tgttgccttc agttgatgaa gtattaagtg ataatagaat
    22561 tgtagaaata ataaatgaat atcctagaag tttagtttta gaatctgtga gagaagtaat
    22621 agatttaaaa agacaattta tattaagatt aaaagaagat gcatcaaact ctgtaacaat
    22681 agagtttggt gaaataatag aatctgcaat agagagagtt aagttaaatt actcattatc
    22741 tttaaaaaaa gttataaatg caactggtac agttattcat acaaatttag gaaggtcttt
    22801 acttagtgaa gatataaaag atgaattatg gtgtgctgct tcaaggtatt caaacttaga
    22861 atacgatttg gataatggag aaagaggttc aagatactct catttaacta gtacaataaa
    22921 aagacttact ggagcagaag atgtactagt tgtaaataat aatgcagcgg cagtacttct
    22981 tgtacttagc acaatggcta aaggaggaga agcaattgta tctcgtggag aattagttga
    23041 agtaggtggt tcatttagga ttccaagtat aatggctctt agtggtgcag aattagttga
    23101 agttggttca actaataaaa ctcatttgaa agattacaag gaggccatta cagaagatac
    23161 aaatgtattg atgaaggttc atacaagtaa ttatagaata atgggattca cagagagtgt
    23221 ttctatagaa gaattggtta atttaggtga aaaatataaa ctacctgtaa tagaagactt
    23281 aggaagtgga gtttttatag atttatcaaa atacggttta tcttatgaac caactgtatt
    23341 ggattctatt cgacaaggtg cagatgtagt aacttttagt ggagataaga tgcttggagg
    23401 tccacaagca ggtattatag ttgggaaaaa agagtatata gaaaaaatga agaaaaatca
    23461 acttactaga gcacttagag ttgataagct tacaatatgt gctcttgaag caacattgag
    23521 aatgtattta gatgagacaa aagctataga aaatatacct actttaaaaa tgttgacata
    23581 taaaattgaa gaattagaag taaaagcaaa taaactattt gaaaaaataa ctgctttaaa
    23641 cttaaatgca aacataaata tagaagatgg attttcacaa gtgggaggag ggtctatgcc
    23701 tcttgaaact ataagtacaa aggtaatatc aataactcct gaacatatga atgtttcatc
    23761 tttagaaaaa aaactaagac ttagtgaagc acatataata gccagagtgt atgataataa
    23821 atatgtacta gatgtaagaa ctatatttga tgatgaattt gatgtaatag ttgcggaatt
    23881 gagaaaagca tttaattaaa taaatttgag ttaataaatg ttaaatttag aagtataagt
    23941 atgaagtttt aattaaaaaa tagctacaca cacttgtgac tttagtcata gcaggtttag
    24001 agggggcaaa aaatgaaaaa tgtagtaatt ggaacagcag gtcatattga ccacggaaaa
    24061 actactctta taaaagcatt gactggaaga gagactgata cgttagatga agaaaaaaag
    24121 agaggtattt caataaactt aggatttact tattttgatt taccaaacaa tagaagagct
    24181 ggaattgttg atgtcccagg acacgaaaaa tttataaaaa acatgttagc tggagcatca
    24241 ggaattgata tagttttact tgttgtagca gcagatgaag gggttatgcc acagacagtt
    24301 gagcatttag atattctaag ctttttaaat atcaaaaatg gaatagtggt tctaactaag
    24361 tctgatactg tagatgatga atttagagag ctagtaaaag aagatatttg tgaaaaaata
    24421 aaaggaactt ttcttgaaga tgctgaaata attgaagttg attctatatc taaaagaggt
    24481 atagacactc ttattgaaaa aatagataaa atgagtgatg atatagaaga taaaaatgaa
    24541 aactctccag caagacttaa tatagacaga gtattttcaa taaaagggtt tggtactgta
    24601 ataacaggca ctttaataga aggaaagata agtatagaag atgatttggt tatatatcca
    24661 caggtattaa aaacaaaaat aagaagtata caagttcatg gagaaaataa agatacagct
    24721 tatgcaggtc aaagaacagc aatcaatata tcaaatgtaa aagtggatga tataaaaaga
    24781 ggagatgttt tagctgcacc aaactcattg gaggaatcaa tgatgttaga tgtaaaatta
    24841 tcattggtaa atcatttaga taaaggatta aagcactggg atagattaag attgtatcat
    24901 ggaactagag agatactttg tagagcagtt ccactagata aagaattaat tgaaagtgga
    24961 gaaagtggct atgttcagtt gagacttgaa gaaagcatag tatctaagaa aggtgatacg
    25021 tttgtagtga gacgttattc tccaatggaa actataggtg gaggtgtaat aattgaccca
    25081 tctcctaaaa aacataagaa atttgatgaa aaagttatag aagcattaaa aataaaagaa
    25141 aaaggcgaac taaaagatat aatagaagag tacttaaaaa ggaatttaaa aaattatcca
    25201 aatataaaag aaataatgag ctatagtggt gctcatgaag aagatgtaaa aagagcgctt
    25261 gaaacactta tatcagaaga taaagtattt attataggaa atatgtatat gcatataaat
    25321 caatacaata aattaaaaga aaatacaata aagcttttaa gtgagtacca taagaagtat
    25381 aggcttagaa aagggatact aaaagaagaa gtaaggtcta agatagagag taattttaag
    25441 actagggaga tggatatttt attagaaaaa ttgtctacag aaaatgctat aaaaatagaa
    25501 aataatatag tatctctatt agattttgag gttattttaa atgataagca aaaagaaata
    25561 gcaaaaaaaa tagaaaaaag attaaaatca tgtggagttt cttctatact gacaatagat
    25621 gaagtttctg aaggaaatca taactatgct gaagtattgg agtcaatgat aggtaataaa
    25681 gtagagaaat tagatgattt gtatataatg gacaaagata tttatgaaaa tgcaaagaac
    25741 attttgataa attatataaa agaaaataaa gaaataacat taggagaata cagagatttg
    25801 attgattcta gcagaaagaa ttgcatgatt atattggaaa acttcgatag aaacaaaata
    25861 actaaaagag tagaaaacaa aagaattttg ttttagcaga aaatgtctgt ttttgcttta
    25921 aggtataaag aaggaattat aatgtcgagt atcgtataat taattagtat aaaatattaa
    25981 ttaagaggaa gtgtaaagta ttgaagaata taatacctga tgtaaaacca gatataaaag
    26041 aaagagaaga catgagcctt ttatttataa taatgggctc agtactttta ttatttatta
    26101 taaaagatat agagaaatat gaaactttat tacatgccat atatacgata tttttagtag
    26161 ctacaggagt aattatattt aatactacaa aatttaaagt agattcattt tctacatttt
    26221 taggattaat ttttatagct actggagtat tggagtgtat atatttattt aattccttgg
    26281 ggattaaaac taagtctata atggagatta acatcacaat atcagcaata acagatttat
    26341 ttcctatact gggtgtatac ttaagtttta aatttgttaa ggataacaaa caaatttatt
    26401 caagtgtaat tttatttata gcaactgcta cattaacaat aacatcttta ttcataatat
    26461 caggaatttg tgactatctg ggaaatggta aatcacaata tgctttagga gttataatat
    26521 ctatattcat aataattatt tctatcatta gtggaattga attaaattca agttcaaata
    26581 aaggtaaatg ggaatatgat gaaaaaaaga tttttaacag aataatagta ataataatct
    26641 tatcaagagt tccaaatcta ttgcatatag ttattgataa tagaaatgta gagaggatat
    26701 tatcacaaat aataataaat gtagccttat attatttata taattacata gtatctaaga
    26761 atattaagaa aacagcactg gaattgcatg atacgaatga agaattaaca aaaaaaacgg
    26821 aaagtttaaa agaaaagaat aaaaaattaa tctatgagac agaaaagata gaggaactta
    26881 agggaatact tatgaaaaga gaatctaggc tacaatctac attagatgta gcagtaaata
    26941 gcatagtagt atttagcaaa gatggagaaa ttacatatgc aaataagagc tttagaaata
    27001 cttttggaga ctataaagaa caagataaat tgactgataa aataaaaaac ttcaatgatt
    27061 tgatagaaag cattcattat gtgtttgttc atgagaagaa tgcagagaaa cttattagta
    27121 caagcgataa taaagtttat caagctatat ttgcaccttt aattattgct tcacaaacag
    27181 aaggagtatt gtgtgtacta gaagataaga ctaagaaaaa ggagtacgaa cgcaaactta
    27241 ttgaagcaaa taagagaagt aaagattttt tagaaagtat tggagatggc atagtagtgc
    27301 ttgaaggtaa taacaaaata tatgttaata atgcatgtag ggaaattttt aaaaataatt
    27361 tagagagtat tgatttttca ttagtatgta aaagtgaaga aagtatagaa aaaagatatg
    27421 taatagatgg tgaagtaaag tatgtagaga tgagtttttc tcagtatact aatgctggta
    27481 ctaataaaac tattattgta atacgtgata caacctctcg aaaaatatct caaataaaac
    27541 ttgaggaaag tcaatcatca tattcaagat ttatagatat attacctgat ggcatatgct
    27601 tattaaaaga agatttaagt ataaactatg caaataagtc gtttttagat atgctagctt
    27661 ttacaaacat agatgatata aaagattcta atattaaact attaatgaat gcgaatactg
    27721 aagaaaaaat gaaatttacg gataatatgg ctaaagtttt aaatgaaaat agaagtatac
    27781 ttttactgga atatgaattg ataaatagtt atgatgatat agtagaagta gaattaagtg
    27841 cattaccttt tgctatatac aatactagat acataatgct tattataaaa gatttagtac
    27901 ataagaaata ttccgagcaa gcagaaaaag aactgcttga aagatttaaa acagataaaa
    27961 tcaaaacgga gttttttgca aatatgtctc atgaattaaa aaccccacta aatgttatat
    28021 ctagttcaaa tcaactggta gattctttct atagaaatga aaagataaaa gattacaata
    28081 acaatattaa atctcatgtg gatttagtta gacaaagttc ttataggctt caaagattaa
    28141 taaataacat tatagattta acaaagatgg agtctgggtt ctatacacta aaacttgcga
    28201 aatacaatat tgtatctgta atagaagact tatttatgaa tatagaagag tatgcactta
    28261 gaaaagacat aaaaattctt tttgatacag accttgaaga aattaatgtt tatatagaca
    28321 aggttgaaat tgagagaatt atgttgaatc tattgtcaaa ctgtattaag tttactgata
    28381 atggaggctg gatatatgta agtattcatt ataaaataga taaagtaatc ataagtgtaa
    28441 aagacactgg agttggaata ccacaagata aattagaatt gatttttgaa gaattttcac
    28501 aagttgataa gactttatcc agaaatactg aaggaagtgg aatagggctt gctatagtta
    28561 agaatctagt aagtcttcat ggtggagata tagaagtagt aagtgaagtg aataaaggca
    28621 cagagttctt aatttcactt ccaatgaagg gatttagtag tgaacattac acagaagata
    28681 agagaatata taatattcaa gaaaaaataa aaatagaatt ttctgatata tactattaaa
    28741 aatgattatg agcaaaaata aaataaaaat actatttgtt taaatattta agttgtaaga
    28801 aataggctat cataaggtaa attattatga tagcctattt ttagctaggt tttatatcta
    28861 tatgtgagaa catataatct ctaaattacc ttcaaaacta aaatctccaa agtcgtttgg
    28921 tagtataaaa tgcataccct tttttaaatt gtattctaca gaattgtgta taagttttcc
    28981 atttccatcc aacacactgt ataaagaaaa aggtgtattg tgtgtaaaat tacattttcc
    29041 aaaaacatct aatttataaa cactaaaaaa ttcgtttgac acaaatgttg tgcatttaaa
    29101 gtctgatgtt gatacgattt tatagtcagt atcgaaattt atatgaggaa cattcgtaac
    29161 atctattgat ttttgaacat gaagttcacg cttattacct gaattgtcag ttctatcata
    29221 atcatagact ctataagtag tatctgagtt ttgttgagtt tctaaaatca atgttccttt
    29281 gcatatagca tgtatagtac cacttggaac atagaaaaag tctccctttt ttatattaac
    29341 ctttctaagt agtttgtccc attcattatt atttaccata tttacaaatt ctttatgaga
    29401 ttttgcatta tgacctatta taatttctgc atcatcagaa caatctatta cataccaaca
    29461 ctctgtctta cctaactctc cattttcatt ttttttagca tattcatcat ttggatgaac
    29521 ttgaactgat aaattatcat ttgcatccaa tatttttgta agtaaaggaa acttatcacc
    29581 aggtgtgttt ccaaataatt ctctattttt attccacaat tcagatagtt ttttaccttt
    29641 atattttcca ttttctatta agcaatctcc atttttatga gagcttatag cccaacattc
    29701 accagttgtt ggcgaatcta tttcataatt aaatttatct ttaagtgcag taccacccca
    29761 tattctatcc ataaaaatag gttttaaaaa taatggttcc ataatcctgt tcctcctatg
    29821 atattaaatt tatcctacaa ataattatat ataaatagat tataaaatca atagtaaaaa
    29881 agttttgtcc cttgaaatga cataaagtat aaggagttat taaataagat aaactatgat
    29941 atataatata aagttctaaa ttggagtgat aattatggag ttgtttaagg cagaaagcaa
    30001 aaaatataaa aaagaatttt taaaattatt ggagcaatgg gttagtatac cttcatttta
    30061 tgatagaaaa actgtatcta aagacatgcc ttttggaaaa ggagtatatg atgcattaaa
    30121 ctggtttgaa aatctaggta gagaaaacaa tttcaaagta aaaaatatag ataatcatgc
    30181 tgttcaaatt gaatatggaa atggtaaaga gtatgtagat atatttggac attgtgatgt
    30241 tgtaaatcca ggtgaaggat gggatagtga accatttaaa ctaaatatta taggtgataa
    30301 attagttgct agaggtgtat ctgataataa agggcctatg atagtgaatt ttttagcact
    30361 aaaaatgata aaggatttaa atattaattt aaaaagaaag gttagattaa tagctggggg
    30421 aaatgaagag agtggattta aatgtataaa gcattattat agcaaagagc catatggtgt
    30481 atgtggattt actccagatg ctaaatttcc tgtattaaat ggagaaaaag gtggagcaat
    30541 aataaagtta atttcaaata tagacgataa atctttatat ataagtggtg gaatagagtt
    30601 taatacaata ccagataaag tttatataaa gaatgtagaa aaattgggta aagataatat
    30661 atgttttgat attaacaata tatcgataaa ttatgataat ggcaattata ttgtgtatgg
    30721 aaaaggtggt cattcatcta aacccgaaaa atcaataaat cctatattag ctacaattaa
    30781 acttttgtca gaaaatattg atgaaaaatg gacaaaagat ttatataagt tgataaatca
    30841 agataatata aatggaaact tatttggttt aaatattgag gggaaatgtg ggattttatc
    30901 aatggtgcca acaataataa atatagtaga tggaaaatta gaagtagttt taagcgttag
    30961 atatccagaa atattaaccc ttgaagatat aataaaaaaa tttaatttgt atatggagca
    31021 aaataatatt aataaatttg agcttattgg ggaaaattta aagcaagcaa attatataga
    31081 tagaaattca aaacttgtaa gaagtttgca tgatatatat ataaagtatt ctggagattt
    31141 aaaaaatgat gttagagtaa ctagtgcagg aagttatgct tctgaaatga ataattcagt
    31201 aatatttggt tgtgaatttc ctgatggtag ctttggcaat gtccatagtg cgaatgaatt
    31261 tgctagctta gatagattta taactgcaat aggtatatat gcagaggcaa taattacatt
    31321 atgtaataaa atataaatac aaaatgtccc ttatatagac atattatata tacaattaaa
    31381 attttacttg atatataatt gaaatcaaag agaaggtgat acaatgataa ctaagaaaca
    31441 tgcaataata gtaaagagtt taagcaataa agatggatat atgacatcaa acgaattagc
    31501 tatcaaattg gatgtgtcta ctaaaactat taaaagatat atagcagatt taaatagtgt
    31561 tttaagcaaa tatgatttgg taatagcttc ttctagaggt ataggataca agttaagcgg
    31621 ttctaaaaac aatatagcca gagctattaa agaagcaaat aaatatattg atggtttttt
    31681 agatgactct gaagagtcaa gaatgtcaaa tataatatgt atgcttatta atagaaatta
    31741 tatgagtata gaagcaatgg cagaagaatt aaatctaagt atagccgcta taaataaatt
    31801 gtctagtaag cttaaaagaa aacttgaaaa gtatgactta gttataaaat ctaaacctta
    31861 ctatggaagc tatatacatg gtgaagaaat taatataagg caattaatta ctgactatgc
    31921 aataaaatta gatgaaaaaa gtaaagtgaa ggtgttttta gatgacatta gtgaaaacga
    31981 tatacactgt atagaaagta ttttagaaaa gcacttaaga gaaaaagata caataatttc
    32041 agataaagat tttaatcttt tgctttcaaa aataatcgtt tctgtattta ggagtaagag
    32101 aggtcatagt aacaatataa acttaatgga tacaagttat agatttcata actactactt
    32161 tatagaaaat ctaatgaaag aaatatctga taaaattgga tttaaactta ttgaagatga
    32221 agtaatttat atttcaaatt attctggtgt aatagcctat aaaggtacac aaggaagaaa
    32281 aaacacaagt gaaattgagg aacgaataag tagtgttata agtagtgcac ttcaagatat
    32341 attccttatt tcaggtagtg attatacaaa agatgatgag tttatggttg caatctctga
    32401 ccatataaag agatttttaa atagagctag agctaatgta aaatcaaata atcctttgct
    32461 tcatcaaata aaagagaagt ttcctatagc ttttaattta gccgtgtttt tatcaaataa
    32521 actagaaact gagtttaatt taaaacttga tgaagatgag cttggatata tagctatcca
    32581 ctttgctgct tcaaatgaaa gaatgaaaaa aaatactagt aagaaaattt gtatagtttg
    32641 tcattatggg ataggaacag gacaacttat ttctgaaaag ctaaaacaga atataagtga
    32701 tttaagtgtt gtgggtgttt atccagttag atatctcgat atggctataa gtcaagatgt
    32761 tgatttgata gtatctaccg ttgaattaaa aggatatgaa aaacctgtct tatatataga
    32821 aaatatattt gatgatagct taattgaaaa cgtaaacaaa gccttttatg aaaaggaaga
    32881 aagaagaaag ataataagca acatgtttga tgaaaaagct tttttcagta taaaggctaa
    32941 tactaaagaa gaggtcataa tttcactttc aaataaacta aaagaaagag attttataga
    33001 ggaaagttct ataaaaagta taatagatag agaaaatatt tcatcaactg aaataggaaa
    33061 tctagtagca ataccccaca caatagtgaa gggagataaa aaatctataa taggagttgg
    33121 aatacttgaa aatccaataa tttgggataa gcaagaggtt cagctagtat ttatggtatt
    33181 ttttaatact aaagaaaaac ataatttctc gatatttaaa tacctttata attttataaa
    33241 agatgaaggc ggagtaagag gaattattaa aatatgtgac tttaataaat taatggcact
    33301 gataggtaat taaggaggaa attaataatg ggaacaaaca tatttaacaa agaatatgta
    33361 tttttaaatg ttgatgctaa gtcaaaggtt gaagttttaa aatttatatc aaaaaaagct
    33421 aaagacttaa acttggcaga agatgagagc ttagtatatg aaggtttaat ggctagagaa
    33481 gaacaattta ctacaaattt aggagaatct atagcaatac ctcacactaa aaatgatgca
    33541 attgaaaatc cagcagtagt agtattgaag tttaatgaag atgttgtttg gaatgaaggt
    33601 gaggataagg ttaaattagc aataagctta cttatgccag gaaaaagtaa agagaatatt
    33661 catcttaaat tattatcatc tctttcaaga aaattaataa ataaagaatt taaagattct
    33721 cttttaaaaa gtgataatgt tgaagaaata tctaacttaa taaatgaagc tttaggttta
    33781 taattaaaca ctaaggttat aaaataaaaa aataattaac taaaaatagg aggaatttga
    33841 aatgaataaa aaattagtag cattatgtgc ttgtccaatg ggactagctc acactttcat
    33901 ggcagcagaa gcaatagagc aagcagcaaa ggcattaggt tatgaagcaa aagttgaaac
    33961 tcaaggtgct gatggtgtac aaaatgagtt aacaagagat gatatattag gagcaactat
    34021 gataatacat gcagtagcaa taacaccaga aggtatggaa agatttgatg gttgtgaagt
    34081 ttatgaagtt gaattacaag aagctataaa aaatgcagaa ggtgttataa aagaaataga
    34141 agaagattta ggaatataat aaatttattg tggaggaaat agaatggcaa ttaagaaaaa
    34201 agttataaac agttcaggtg cttctacaac aggaggaaat aatgtaggaa aatctactcc
    34261 atctagtcca actcaatcaa agggaaatgg aaaatggaaa gaaataagta aacatataat
    34321 gacaggtata tcatatatga taccagtttt agtaatgggt ggacttatag gggccttatc
    34381 tcaattaata ccatatgcta tattaggact tgacccatct gtaggtatag ttgatgctat
    34441 gaactcagga gaatttacag ggttcaagct tagtctttta aatatagcac aattaatgtc
    34501 aaatttcgga tttactttgt ttggatttgc gataccactg tttgcagcat tttgtgcaaa
    34561 ctctataggt ggaaaaacag ctttaattgc aggatttata ggtggatata tagctaataa
    34621 acctgtaggt gtaccacaat tcgttgatgg acaatggact gaagttgttc cagttgcatc
    34681 aggattttta ggtgcaatac taatagcatt tataatagga tattttgtaa aatggttaaa
    34741 taaatctata aaagtttctc ataactggtt agcttttaaa acaacatttt taataccatt
    34801 aatagcgtca ttagcttgta tggtattgat gatatttata ataactccat ttggtggatt
    34861 aattaatgaa agtatgaaaa acttcttaac agcagcagga gcagctggag aatatgtata
    34921 tgcaacagct ttggcagcag ctacagcatt cgacttagga ggaccaataa ataaagcagc
    34981 aggattcgtt gcacttggtt taactacaga gaatgtactt ccaataacag caagaacaat
    35041 agctatagtt gttcctccaa ttggattagg attaacaaca ttattagata aaagattagt
    35101 aggaagaaga gtatatgata gacaattcta tcaagcagga aaaacttcta tattcttatc
    35161 atttatgggt atatctgaag gagcaatacc atttgcactt gagagaccag gttttgttat
    35221 acctttaaac atagttggaa gtgtaatagg agctattaca ggtataattc taggagcaat
    35281 tcaatggttc ccagagtcag ctatatgggc atggcctcta gtagataact tatttggata
    35341 tataataggt atagcagttg gtgctatatt catagcagta ggaaatatat tctatagaaa
    35401 taaattaata aaagatggta aacttgttgt agattatatc gactagtatt taatttttac
    35461 tatctataaa taactttggc tggtaatatg gttttaatca atattaccag ccttacctat
    35521 atgattaatt aaaggcggtg gagataagat atggataaga tagaaaaagt aagagagtat
    35581 tttagagaat atgatataga tggatttctt ataaatagtc ctacaaacaa gttttatgtt
    35641 ggaaatttat ttagctcttc aggttatgtt tttataacga aagaatcaca atatataata
    35701 gtagatttta gatattttga agaaattaaa agaaaatcta gtttgtttaa tgtagtatta
    35761 atggacaaaa ctagaactca ttttgatata attaatgata tttgtagaga gcagaatata
    35821 aaggaaattg gatttgaggg aaatgaagta tcttttgatt tatatagaag tatgagtaat
    35881 aagttaagtg caaccttaaa atcagttgat ttatctacat taagggaaac aaaaaatgaa
    35941 gacgaaataa aatatataaa aaaagcttgt gaaatagttg atgctacatt ttatcatata
    36001 gtagacttta taaaggttgg tatgactgaa aagcaagttg aaaatgaaat tgttagaatt
    36061 ataaaagatt taggtggtca aaaagaatct ttcgatacaa tagttgcatc tgggcttaga
    36121 ggagcattac ctcatggaaa agcaagtgaa aaagttatag agtatggaga ttttgttact
    36181 tttgactttg gtgctaagta taataattat tgttcagata ttaccagaac catttgtatg
    36241 ggtactataa ataaagagtt agaagaaata tataatatag taagaaaagc taatgaagag
    36301 tgtataaggg ttttaagacc gggaatgact actggagaga tagataaagt tgcaagagac
    36361 ataattggtt catatggata tgctggtaat tttggtcata atctgggtca tggtgtagga
    36421 attatggttc atgaatatcc tgcattagct cctgaatcaa atgaagtatt aaaagaagga
    36481 atgattgtaa ctatagagcc tggtatatat gttccaagtc ttggaggcgt aagaatagaa
    36541 gatgatgtac ttataacaca agatggatgt atgagattga ctacatctac taaggattta
    36601 atagttgtaa gctaaaaata aattagataa atattttata acaataagaa aagaagaggt
    36661 aaatttaaat gtataatttt gatgataaac cagatagagt aagtgaaaag tgtagaaaat
    36721 gggatttaaa tattataaga gataaatttg gagatattag agaagatttt gtaccaatgt
    36781 ggattgctga tatggatttt aaaataccaa cagaaataga aaacaaattt attgaagctg
    36841 taagaagagg tgtatttgga tatacatact gttatgatga attttatgat tcagttattg
    36901 gttggcaaaa aaatatgcat gaggtaaatg tagaaaaaga atggattaca ttaacttatg
    36961 gaactgtatc aacacttcat tatgtagtac aagctttttg taagaaaggt gatagtataa
    37021 tattaaacac accagtttat gacccctttg aatcatcagc aaaaaaacag ggtgtaaatg
    37081 taatttgtaa tacactagat gttgtaaatg atagatatta tataaatttt gataaattag
    37141 aagttcaaat taaagaaaat aagcctaaat taatgatgtt ttgtacacca cataatccat
    37201 ctggaaggat atggacaatt gaagaaatga caagggtagc aactatatgt aaagaaaata
    37261 atgttatttt ggttgcagat gaagtacatg cagagcatat tcattatggt aaatttaatt
    37321 caatacttaa gattggaaaa gagcttttag aaaatgttat tctactgaca tcaccaaata
    37381 aaggatttaa tcttggtgga ttaaagactt cttattcaat agttataaat aaggatataa
    37441 gggacaaatt tagaaataaa ttaaagcaaa attctataac ttctccaaat gtgtttggaa
    37501 taataggatt gataaccgct tataatgaat gtcatgagtg gttaagtggt gtaaatgaat
    37561 atataaaatc aaattatgaa cttttagaaa cttgggtaaa taaatataat aagataaaat
    37621 tgatgaaaat ggaatcatct tacttagctt ggatggatat aagtggatta ggtattagtg
    37681 caagtgagtt tacagataaa ttggctatag atacaggagt attattagaa gatgggagtc
    37741 attttgtaaa agatggtgag aagtatgtta gaataaattt aggaactcaa aaggaaaatg
    37801 taattgaagc tttaaataga atggatttat ttttaaagtc tttataaaaa tttggttgaa
    37861 ttagtatttt atataaattt aaactattaa tatggtatat acataagtag aatttgtttt
    37921 agattactta agtatatacc atattttata taaaaattaa atttgcatta gtcatgtgat
    37981 aactatttaa attcatatgt attttgtatg tataatagat ataattatta tttaaactta
    38041 ttgtttattt aatttttcaa tagaaaaaga tttatctgta agtttaaaat tatcttttat
    38101 acccttagtt aggtatactt tcttatcctt agttatgaat atagcatcta cattatcaat
    38161 tttattaatt aaatccatac ctttttcaag accaagtcca aatgtagaag tggataaggc
    38221 atcacaatta atagatttat cagagattat tgtaacacta cttaaattgt tttcaaaagg
    38281 ataccctgta aatggattta acatatgatg atatattttt ccgttttttt cgataaatct
    38341 ttcataaatt ccagaagtaa caacagattt atttgtggtt tctatgtttc ctatggattc
    38401 accattttta ctagttgggt cttgaatgcc tattttaaaa ggtttatcat ttttaccatt
    38461 ttctaaagta aatatatttc ctcctaagtt tactaaacct ttttttacat cctctgattt
    38521 taaataggct acaattttat cagcagcata tcctttagct atggcaccta aatcaatttt
    38581 catattttct ttggttaatt ttatagattt atttttttca tccaatatga tgttcttata
    38641 atcaataaga ggcagaagtt tttgtatctc agaatcactt ggaactttag ctttatctgt
    38701 tccaatagcc cataaatttg ataatggacc aattgttatg tcaaaatatc catctgataa
    38761 tttggaatat tgtatagctt cttttacgac gaaaaatgta tcatcagaaa ctctgacaaa
    38821 ttttttacca gcattatcat ttattttaga aacatcactt cctggtatat gagtgctcat
    38881 tttgttgtca atatatctta aaattgaatc acattcattt aagattttat cacttttgga
    38941 ttcttttaca ttaaatacag taacttcgtt tactgtacca aggtagtagc tagttttact
    39001 atatttagta ttctggcttg aactcctaaa aaatgaaaat aaaattaatg ctaaaatcaa
    39061 tatcacaaaa ataaatgtga tttttttctt attcatggta tattcctcct atgaaattgt
    39121 gttattattt tattataaca taatttcata ggaggacaaa aaatgaagaa aaaagacatt
    39181 cttcttattt taggcatttt ggttgttatt acagcatgtt atgggataat aaatgttata
    39241 aattctaaaa atgctggaaa tattgagatt tatgtagata ataaattgta taaaactgtt
    39301 tcaataaatg ccaaagaaga atttaaaata gagaatagag gggggtataa catagtaaag
    39361 attcatgata aaggggttga gatagtagat gcttcatgtc ctgataaggt ttgcgtacat
    39421 actggattta taaacaagcc aagtcaaagc atagtttgta tacctaatag agtgagcata
    39481 aaaataaata caaatgaaaa gaatgataat caagaagata taatttctaa ataaaataat
    39541 aaaaataatt gaaaggaatt ggtgataatt atggttaact taggaaacga ttgggatgaa
    39601 ttattgaaag aagaatttga aaaagattac tacttaaact taagaaaatt tcttatagaa
    39661 gaatataaaa cacgccaaat tttcccaaat atgcacaata tatatgaagc tttaaaacac
    39721 acttcataca aagacacaaa agtcttaata ttaggacaag acccttatca tggagataat
    39781 caagctcatg gtttagcttt ttcagttcaa ccacaagtga aaactccacc atctttatta
    39841 aatatgtata aagaattaaa agatgattta ggttgtttta tacctaataa tggatattta
    39901 atgccttggg cagaccaagg agtgttactt ttaaatactg ctcttactgt tagagcgcat
    39961 gaagctaatt ctcataagaa caaaggatgg gaaattttca ctgatagagt aatatctatt
    40021 ttaagtgagc gtgaagaccc agttattttt gtactatggg gaagtaatgc acgtaaaaaa
    40081 gtagagttga ttgatacaag taaacattat attttggaag cacctcatcc aagcccattg
    40141 tcagcaagta aaggcttttt tggatgtaaa cacttctcta agataaatga aattttgaaa
    40201 aagctaggaa aagaacctat taattggcag atagaaaata tataaaagta aaaggtaaca
    40261 aaatgataac aaaacaacaa taaacaatga cattgttgta actaatagaa atatgcttga
    40321 atggttgaaa atactatgta tgcatatttc tatttttttg tcaagttgac aactcttgaa
    40381 aaaatagtta caatttagtt acacaaaaaa cacaaataaa aaaagaaact tacaaagcag
    40441 aattttatga agcaaaatat atagtgtaaa aaatcttagg gaggaaattt tatatgaatt
    40501 ttaaaaaatt gagtaatgct aaagtctcgg taatggcagt tgttttatca gttggaatat
    40561 tatcgggata ctctgcgctt aataaacaag taacattagt agtaagggga gaagaaagaa
    40621 ctatatcaac atttagttct aatgtaggag accttttaga atctcaaaac attagctatg
    40681 atgcaaatga tatagtaact ttagatgaaa atacaaagtt atctaatgga gataaaatag
    40741 aagtaattga tgtaaaggaa aaaacagttg tagaaactaa agacattcca tttgaagtta
    40801 cagttgttga agatggctct ttattaaagg gtgattctaa ggttaaagaa gaaggaaagt
    40861 ctggtaaaag tgaattaata tataaagaaa cttatcacaa tggaaagaaa gtagaaaagg
    40921 aatttgtgaa aaaggttgtt aaagctgagc cagtaaataa ggttgtaagc aaagggacaa
    40981 aggtagaaat gcaagtagcc tcatcaaggg gagaaagttc aagaagaatg gcttcatcat
    41041 catctagtag cagtagtagt aacaatggta aaaatatgag agtggtagca actgcatata
    41101 ctggtcacag tataactgca acaggaacaa aacctaagtg gggaacaata gctgtagacc
    41161 ctaaggttat accatatgga actaaagttt atatacctca atttgacatg gtgtttactg
    41221 cagaagactg tggtggagca ataaaaggaa ataaaattga tatctacatg aatgattcaa
    41281 aaacagttta taactgggga agaaaaacta tagatatacg tatattaaaa tagttaaata
    41341 atttatattt ttactggaaa ttatattttg attatataga gaagaaactc tttatagatt
    41401 ctaaaaatat aatttccagt tttttatttt ggatatgtaa taaaacaggt aaattttaac
    41461 catgtataga taaaagctat ttaaattata atttactatc atataaatct ataaaaattg
    41521 ttgcaaagag tcaaaatata tgatattata gatttcgttg agaaggattt cattatggga
    41581 gtggatgggt gccggtgtgc cctctagtct tcaaaactag tatgaggggt taagagcctc
    41641 ttaggtgggt tcgattccca cgcattcccg ccaatatgta aaaggtatct tttaattaaa
    41701 agataccttt ttaaatttat tttaagataa tttattttta ataatatata atgttttgta
    41761 ttatataatg taaattttct aatactatta tatatttaga taaaaatcta ggagtgtgga
    41821 aagatggaaa ataattttac aacgagaact agttttctag ttggagatga tgggataaaa
    41881 aaacttaata attcaaatat aatagtattt ggagttggag gagttggtag ttttactgtt
    41941 gaagctttag caagagctgg agtaggcaat attactattg tagattttga tgatgtagat
    42001 ataaccaata taaatagaca aataccagcc ctacattcaa ctgtaggaag atacaaagtt
    42061 gatgtcatgg aagaaagaat attagatata aatcctaata tcaatataaa aaaaataaga
    42121 agtctttata ataaagatac tagtgacgaa atactaactg aaagatatga ttatgttgtg
    42181 gatgcgatag acatggtaag ttccaaaata catctaattg aaacttgtga aaaaaaagga
    42241 ttgaaaataa taagttctat gggtatggga aataaattag acccaactaa aatagtcgtt
    42301 acagatatac acaaaacaag tacttgtcct ctagctaaag ttatgaggaa agaacttagg
    42361 gatagaggta tcaaaaagtt aaaagtagta tattctactg aacaaccaat agaacttaag
    42421 aagaaagtaa tgaatggtag aaaagtaaca cctggtagtg tatcatttgt cccatctgta
    42481 ggaggtttaa taatcgcatc tgtaatagta aatgaactat taggacaata agttttaaat
    42541 attttaattt ataaatttta tataaaaatt tgaaggctag aagcatgaat acaaagcaat
    42601 tttatattca tacttttagc ctaattattt tatattacct tattttatgt agttgtagtt
    42661 ttatttttta gattatgtat ctatttcata attatatttt gtaaatataa tttctccatc
    42721 actataaaaa acaatatctc ctattctatc tgttctataa attgatacat ggttttgttt
    42781 tagattatta ataacctccc tatgtggatg accatattga tttttatatc cacatgatat
    42841 tgcagctata tctggactaa ttttttttat aaattctaaa gatgaagaag aattacttcc
    42901 atgatgacct atttttataa aattaatatt atttaattta aaagaatgta aaatttcttt
    42961 ttcattttct tcttcagcat cacccataaa taaaaaagaa ttatcattaa aagttaattt
    43021 gaaaactata gaatttaaat tagattcttc ttgtatatat gatggactta atacatatat
    43081 atttatattg ttatctatgt ttaaaacatc atttttatag agatgctgaa tagataagtt
    43141 tttatccgta caagaattta ttagattttg ataagcctca ctatcagttg attgttcagg
    43201 catatatatg gagtttacat taaatttttt tataatatta tcaagacttc ctatgtggtc
    43261 tgaatctgga tgagttgcta ttataatatc aatagttttt attctttttt gtctaagata
    43321 actaataatt atattttcgc tatcttcatc acctccatca attaatatat tcttatttgt
    43381 gggtgtttga actaaaatac tatcaccttg tccaacatca atcatatgta tagatagtag
    43441 agattttttg tcacagccga ttaaaagtga tattataata agtattgata aaaaaatttt
    43501 atttctcaat aaaaaatcac tccattcatt acatgtttat ataaa
//
LOCUS       DBGJFF010000020        42545 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.020, whole genome shotgun sequence.
ACCESSION   DBGJFF010000020 DBGJFF010000000
VERSION     DBGJFF010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 42545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42545
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.020"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            485..1684
                     /gene="hadA"
                     /locus_tag="ACOIAF_001111"
     CDS             485..1684
                     /gene="hadA"
                     /locus_tag="ACOIAF_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocaprenoyl-CoA:2-hydroxyisocaproate
                     CoA-transferase HadA"
                     /protein_id="HGZ2668131.1"
                     /translation="MLLEGVKVVELSSFIAAPCCAKMLGDWGAEVIKIEPIEGDGIRV
                     MGGTFKSPASDDENPMFELENGNKKGVSINVKSKEGVEILHKLLSEADIFVTNVRVQA
                     LEKMGIAYDQIKDKYPGLIFSQILGYGEKGPLKDKPGFDYTAYFARGGVSQSVMEKGT
                     SPANTAAGFGDHYAGLALAAGSLAALHKKAQTGKGERVTVSLFHTAIYGMGTMITTAQ
                     YGNEMPLSRENPNSPLMTTYKCKDGRWIQLALIQYNKWLGKFCKVINREYILEDDRYN
                     NIDSMVNHVEDLVKIVGEAMLEKTLDEWSALLEEADLPFEKIQSCEDLLDDEQAWAND
                     FLFKKTYDSGNTGVLVNTPVMFRNEGIKEYTPAPKVGQHTVEVLKSLGYDEEKINNFK
                     DSKVVRY"
     gene            1723..2523
                     /gene="hadI"
                     /locus_tag="ACOIAF_001112"
     CDS             1723..2523
                     /gene="hadI"
                     /locus_tag="ACOIAF_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyisocaproyl-CoA dehydratase activator
                     HadI"
                     /protein_id="HGZ2668132.1"
                     /translation="MYTMGLDIGSTASKGVILKNGEDIVASETISSGTGTTGPSRVLE
                     KLYGKTGLAREDIKKVVVTGYGRMNYSDADKQISELSCHARGVNFIIPETRTIIDIGG
                     QDAKVLKLDNNGRLLNFLMNDKCAAGTGRFLDVMAKIIEVDVSELGSISMNSQNEVSI
                     SSTCTVFAESEVISHLSENAKIEDIVAGIHTSVAKRVSSLVKRIGVQRNVVMVGGVAR
                     NSGIVRAMAREINTEIIVPDIPQLTGALGAALYAFDEAKESQKEVKNI"
     gene            2538..3764
                     /gene="hadB"
                     /locus_tag="ACOIAF_001113"
     CDS             2538..3764
                     /gene="hadB"
                     /locus_tag="ACOIAF_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     HadB"
                     /protein_id="HGZ2668133.1"
                     /translation="MSEKKEARVVINDLLAEQYANAFKAKEEGRPVGWSTSVFPQELA
                     EVFDLNVLYPENQAAGVAAKKGSLELCEIAESKGYSIDLCAYARTNFGLLENGGCEAL
                     DMPAPDFLLCCNNICNQVIKWYENISRELDIPLIMIDTTFNNEDEVTQSRIDYIKAQF
                     EEAIKQLEIISGKKFDPKKFEEVMKISAENGRLWKYSMSLPADSSPSPMNGFDLFTYM
                     AVIVCARGKKETTEAFKLLIEELEDNMKTGKSSFRGEEKYRIMMEGIPCWPYIGYKMK
                     TLAKFGVNMTGSVYPHAWALQYEVNDLDGMAVAYSTMFNNVNLDRMTKYRVDSLVEGK
                     CDGAFYHMNRSCKLMSLIQYEMQRRAAEETGLPYAGFDGDQADPRAFTNAQFETRIQG
                     LVEVMEERKKLNRGEI"
     gene            3764..4891
                     /gene="hadC"
                     /locus_tag="ACOIAF_001114"
     CDS             3764..4891
                     /gene="hadC"
                     /locus_tag="ACOIAF_001114"
                     /EC_number="4.2.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     beta"
                     /protein_id="HGZ2668134.1"
                     /translation="MEAILSKMKEVVENPNAAVKKYKSETGKKAIGCFPVYCPEEIIH
                     AAGMLPVGIWGGQTELDLAKQYFPAFACSIMQSCLEYGLKGAYDELSGVIIPGMCDTL
                     ICLGQNWKSAVPHIKYISLVHPQNRKLEAGVKYLISEYKGVKRELEEICGYEIEEAKI
                     HESIEVYNEHRKTMRDFVEVAYKHSNTIKPSIRSLVIKSGFFMRKEEHTELVKDLIAK
                     LNAMPEEVCSGKKVLLTGILADSKDILDILEDNNISVVADDLAQETRQFRTDVPAGDD
                     ALERLARQWSNIEGCSLAYDPKKKRGSLIVDEVKKKDIDGVIFCMMKFCDPEEYDYPL
                     VRKDIEDSGIPTLYVEIDQQTQNNEQARTRIQTFAEMMSLA"
     gene            4939..6072
                     /gene="acdB"
                     /locus_tag="ACOIAF_001115"
     CDS             4939..6072
                     /gene="acdB"
                     /locus_tag="ACOIAF_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative isocaproyl-CoA dehydrogenase AcdB"
                     /protein_id="HGZ2668135.1"
                     /translation="MLYNKEQELLRKAVRDFVSKELDTLPAEMDKTGVMPKELIKKLA
                     DAKFISSNIPEEYGGGGAGYVSYAIVMEEIARRCASTATFVTAGSSLASLPILYNGTE
                     EQKQKYLKGIATGELIGAFGLTEPGAGSDAGGQQTTAELVGDHYILNGRKTFITNGPF
                     CDVAIVIAVTDRSKGLRGTSAFIVESKWDGFSTGAHEDKMGIRGTETSDLIFENVKVP
                     KENLLGKEGQGFKIAMGTLEVGRIGVAALALGIAQGALDEAVKYTKQRVQFGKPIAKF
                     QNTQFTIADMETKVCAARGLVYDAAQKRDAGMRVAQESAMAKYYASEIANEVAYKALQ
                     LHGGYGFIKDYEIERMYRDARIVSIYEGTSEVQKMVISSNVLK"
     gene            6105..6890
                     /gene="etfB"
                     /locus_tag="ACOIAF_001116"
     CDS             6105..6890
                     /gene="etfB"
                     /locus_tag="ACOIAF_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta"
                     /protein_id="HGZ2668136.1"
                     /translation="MKILVCVKQVPDTNEVRINKETGTLIRDGVPSILNPDDANALEE
                     ALKIKDEHDDVTITVITMGPPQADFMLRECLAMGADDAILLSDRAFGGADTWATSNTI
                     AAGISKVGDYDIIFAGRQAIDGDTAQVGPQIAEKLDIPQVTYVQDFKIEGKDIIVQRQ
                     LEDGYELIKVSTPVLLTAVKELNTPRYMSVDKIVKAYKHDVKVWTIDDLDVNKEEVGL
                     KASPTKVFRSFTPEPRGKGEILEGKADEIASKIIIGLKQKHII"
     gene            6909..7946
                     /locus_tag="ACOIAF_001117"
     CDS             6909..7946
                     /locus_tag="ACOIAF_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HGZ2668137.1"
                     /translation="MNDIKDLSSYKNVWIFAEQREGKIAPVVIELLGEGRKLAKEVDA
                     ELCAILLGKDVDGLAKELITFGADKVYVADDALLEKYTTDAYTKVIKDAIDEIKPEIM
                     LFGATHIGRDLAPRIASRVGTGLTADCTKLEIDPEDKKIKQTRPAFGGNIMATIICPN
                     HRPQMSTVRPGVMDKAEKDETRTGEVIALDYKITQDDIRTTVLETVKTKKDLVSLTDA
                     NVIVSGGLGLGGPEGFEMLKKLADKLGGVVGSSRAAVDAGWIDHSHQVGQTGTTVKPN
                     LYIACGISGAIQHLAGMQSSDFIIAINKNPAAPILEIADYGVVGDLHEIVPMLIEKLD
                     SVDDLLEAIKA"
     gene            8429..10060
                     /locus_tag="ACOIAF_001118"
     CDS             8429..10060
                     /locus_tag="ACOIAF_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HGZ2668138.1"
                     /translation="MDNNLIGLIEHVENPAILCKESGEIIYCNHLIDSIFSFLDIKKP
                     RNINELDSNFDKTEILTDSKKKIAFRELRMTAHIYNMKDNNNENNIVYLFEKSLISDK
                     VIEDIIEHIDEVVVVFNKDGVIEKMNTVSDEILPFKRTEVLGRNITDLVRQGLVEEPI
                     ILNMLKVKKKIYRNIVYPDGKLIAYTAVPRWDSKGKLTGGVLTGRDISRVIKLESQIK
                     YSDISEDTEYISQSKIMDNIKKVVKRAAASDSSIFINGESGVGKEIIARTIYKYSSRR
                     DKPFIAINCGAIPNELLESEFFGYEEGSFTGAKKKGKKGLFEEANGGTIFLDEIGELP
                     MQMQKKLLRVIQENTITRIGGSKPIKIDVRYISATNISHEDLRNNLKFRQDLYYRLSV
                     IPVKIPPLRERKEDIVPLVNYFLKLYNEKYNREVEVSPKVIELLEEYSWPGNIRELKN
                     IIERFVVLSAKNVIGEDEFNMLINLDMIDNETDDLSPIVVNGIMNLNDAYKIVDQIMI
                     SKAINKYGSITKAAEVIGIYPSTIHRKIKSGHIHV"
     gene            complement(10195..11121)
                     /gene="fba"
                     /locus_tag="ACOIAF_001119"
     CDS             complement(10195..11121)
                     /gene="fba"
                     /locus_tag="ACOIAF_001119"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0030388 - fructose 1,6-bisphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HGZ2668139.1"
                     /translation="MALITTKEMFKKAYEGGFAIGAFNISDLEQLQGVLKAAKAKNSY
                     VMIQASMSAVKYAGPHTLVEMVKAASDEIGVDVALHLDHGPNMDAIKTCIDAGFSSVM
                     IDGSHFDFEENVRITKEAVEYAHSKGVVVEAELGVLAGTEDDVTSDVHKYTQPAEAVE
                     FVERTGVDSLAIAIGTSHGAFKFKGEAKLRFDILEEIQSKLPGFPIVLHGASAVDQNA
                     VATCNEFGGNIAGAKGVPVDMLRKASSMAVCKINMDTDLRLAMTAAIRKFLAENPKEF
                     DPRKYIGAGRDAIQAVVESKIDDVLGSANSIN"
     gene            11451..11780
                     /locus_tag="ACOIAF_001120"
     CDS             11451..11780
                     /locus_tag="ACOIAF_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1904 domain-containing protein"
                     /protein_id="HGZ2668140.1"
                     /translation="MPQIKIRGINENDICKISEKMINDLVEAVKCPRDYFEIECIKSV
                     AIRDGKIADVYPFVEVAWFDRGQEVQDIVARIITDSIRNNLDVESMDLAFTVFEKEKY
                     YENGEHF"
     gene            11780..12502
                     /locus_tag="ACOIAF_001121"
     CDS             11780..12502
                     /locus_tag="ACOIAF_001121"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HGZ2668141.1"
                     /translation="MAKKQRIDKILSNLGYGSRSEIKKYCKQGSVVVNGSEVSNPGTQ
                     VDTENDEILFNGEEVIYREYIYLMMNKPAGYISATTDKYDPTVLDLIDLSYLVFEPFP
                     VGRLDKDTEGLLVLTNDGKLSHRVLSPKKHVPKTYYAKIDGVVTEEDVEAFLEGVVLD
                     DGYKTMPSQLNILKSDDESEIELTIHEGKFHQVKRMFESVGKKVVYLKRLSMGNLKLD
                     ESLELGEYRELTDEEVKLIEER"
     gene            12529..13890
                     /gene="rlmD"
                     /locus_tag="ACOIAF_001122"
     CDS             12529..13890
                     /gene="rlmD"
                     /locus_tag="ACOIAF_001122"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HGZ2668142.1"
                     /translation="MSVKKKDVIEFEVDKMEFGGTSLSQVGDRVIHMKGGISGQKVRA
                     GVKKVRSKKAEVKMIELLENSPLETEETCKHFRECGGCTLLSVPYEKQLEIKEKQVMD
                     LFLKQDLFGFQFLGIEGSPENKYYRNKMEYTFGDEVKNGPLTLGLHKKGKHIDIQTVE
                     ECMLVDEDFSKILVSSVEFFNEKKLPYYRTMNHKGYLRHLVVRKGIHTNEIMVNIVTS
                     SQEDFDMYEFKDMLLGIDLKSELVGVLHTINDGLADAVQCDELRVLYGRDYIQEEILG
                     LKFKISPFSFFQTNTKGAEVLYSIARDFIGDYNDKVVFDLYSGTGSIGQVMAGAAKKV
                     YGIEIVEEAVVAANENAKLNGLTNCEFIAGDVAKVVKDLKDKPDLIIVDPPRPGIHKD
                     AIRDICGFGAKEIVYISCNPKSLVVDLVDFKGYGYEIKMVKCMDMFPNTPHCETVVKL
                     IRE"
     gene            14401..15435
                     /locus_tag="ACOIAF_001123"
     CDS             14401..15435
                     /locus_tag="ACOIAF_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHYH domain-containing protein"
                     /protein_id="HGZ2668143.1"
                     /translation="MFKKKLIAVAMSITMISVGSFSYAHSGRTDSSGGHRDNKNKSGL
                     GSYHYHCGGYPAHLHNNGGCPYTGGGSSSGTTTSVNNEEKQKRSVGEKGYNQGYEDGY
                     KGNYSSSNYSGDYSDTYESKYSEGYEKGKAKLEEEEKVAKETGYNLGLTGAKSNNTYE
                     KEVLKNAYDTGYSTGYNEYKTKKIEEYKAKGIEDSNKDKEKMTFEENIDSEFIDAYNN
                     AYDEIQEQLKNDYTTQGFESAIKGERFDTSTIGNVKYANWFKEGYDNGKVKLPKAKES
                     VYNQGYNEEDFSIPDEMKSIETKLKGVYDEGLEKREEEKSRNATYGVGAGTAAVVAGV
                     VYKKKKRKKI"
     gene            15522..15725
                     /locus_tag="ACOIAF_001124"
     CDS             15522..15725
                     /locus_tag="ACOIAF_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HGZ2668144.1"
                     /translation="MGFNFRKSINLGGGLKLNLGKKSVGISAGVKGARVSVNSKGRKS
                     ATLSIPGTGISYTKTSTKKRKPK"
     gene            15896..17056
                     /locus_tag="ACOIAF_001125"
     CDS             15896..17056
                     /locus_tag="ACOIAF_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp27"
                     /protein_id="HGZ2668145.1"
                     /translation="MKLSKTLLAIGMSVSLLIASCPTSNALSSINSIKGTNKYETAGL
                     IADKQNYDIAILINSENGLSDGLSASGLSGAKNAPILLTKKDTIPSETLKRLEKVKEV
                     YLIGGKNSIGASVQKILSDKKISINRISGTDRIDTSHKVAKEIGRVSTVKKVILTNAF
                     KGEPDAMSVASIAVRDKSPIVLTDGNTTSFVTDGLETYAIGGKVSMSDKLINETKATR
                     IGGVDRYDTNKQIINKFYNGSKEFYIASGTDLVYSLVGSTIAQKTPIVLVGNGSNKSI
                     LKNASKITAIGNIDDVVIKQCLNITNNVGDSNTGIVKDTNSNNTNKPSNEPSSNNPGL
                     TDKSTVYVTPNGKSYHLTKNCSTLKRSKTINKVTLKQAKSQGKIDPCNVCVK"
     gene            17316..17456
                     /locus_tag="ACOIAF_001126"
     CDS             17316..17456
                     /locus_tag="ACOIAF_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668146.1"
                     /translation="MENLMMSIITGVIASYIYDKLKSHSERPTKSGWELNIKFQKSKS
                     SK"
     gene            18404..19804
                     /locus_tag="ACOIAF_001127"
     CDS             18404..19804
                     /locus_tag="ACOIAF_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 interaction domain-containing protein"
                     /protein_id="HGZ2668147.1"
                     /translation="MNQEIFFKIMNEILENIDEGVHFVNKDGKTIIYNNSVQKMEKMN
                     RHDILTNSFFDIVNKMKINRSTLLEVLEKREVIKDNIQKYLDKNGKEITAINTTIPID
                     IKGEFVGALEISKNMTTIENLLNRNYKKNLVNKVSIRQKKGYEFDDILGESNLIKEAI
                     AKSKKACKSDASVFIYGETGTGKELFSQSIHYGSNRRNYPFIAQNCAALPESLFEGIL
                     FGTSKGGFTGAIDRPGLFEQANKGTLLLDEINSMPIMLQAKLLRVLQEGYVRRVGGTK
                     DIPIDVRIIATTNESPKVILNEHKMRKDLYYRLNIIHIKIPPLRDRKEDIPLLCKKFI
                     SKYNKKLNKKVVGISDEAIKILKNYDWQGNIRELENIIYCTMSMMEDEVQIETSMINL
                     NDYYTLKENKESNNYSLNDLEDKTLSKIISEIEEKYIYESLEKASYNITKAASILGMN
                     RQNLQYKIKKYNIKIL"
     gene            20060..21127
                     /gene="ord"
                     /locus_tag="ACOIAF_001128"
     CDS             20060..21127
                     /gene="ord"
                     /locus_tag="ACOIAF_001128"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,4-diaminopentanoate dehydrogenase"
                     /protein_id="HGZ2668148.1"
                     /translation="MRKVRVGIWGFGAMGIGMANMILKKEGIEIVSVCSRSTSGKSMY
                     DVLGIERGERPEVIINKNYEEVFREKSVDVVLLATDSFTKKAFDKIIFLLNRKINVIS
                     TAEQMAYPQADDADLAKKMDEVAKENGVSILGTGINPGFVLDLLVLALSGTCEEVTSI
                     KAKRVNDLSPFGKSVMVEQGVGVTREEFIKGVEDKTIAGHVGFVESINMIADGLGWKL
                     DKIEQTKEPIMTTVDRKSKYGEALAGNVAGCRQCGYGYVNGEVLIEMEHPQQIIPEAE
                     GIKTGDYVSIKGIPNIDLQINPEIPGGVGTYAMIVNSIPLIINARPGLKTMLDIPVPR
                     AIMGDIRNQIEVELEEESKAN"
     gene            21204..21512
                     /gene="ortA"
                     /locus_tag="ACOIAF_001129"
     CDS             21204..21512
                     /gene="ortA"
                     /locus_tag="ACOIAF_001129"
                     /EC_number="2.3.1.263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-oxopentanoate thiolase subunit OrtA"
                     /protein_id="HGZ2668149.1"
                     /translation="MDAKINDWVIIHNIVLTPEERAPQVPEDTKKVSLEMWVKGFIQS
                     DASIGDLVEVKTITGRLVKGNLLKINPYYTHDYGKCIPELLQIGIQAKEILFGGVYNE
                     "
     gene            21505..22935
                     /gene="ortB"
                     /locus_tag="ACOIAF_001130"
     CDS             21505..22935
                     /gene="ortB"
                     /locus_tag="ACOIAF_001130"
                     /EC_number="2.3.1.263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-oxopentanoate thiolase subunit OrtB"
                     /protein_id="HGZ2668150.1"
                     /translation="MNNSTSMKDMSYQAVMGRNNEIMKNAIGLDYSSFEQEGIGFDYE
                     KMMSETGYTLQDIEAIQSQYAVGNTPLIELKNLTKLARKCAKEGKGARIFVKDEAMNA
                     SGSFKARRAATAVYHAKQMGYKGVIAATSGNYGAAVASQAAMQGLKCIIVQECYDSNG
                     VGQPEIIEKARKCEAYGAEVVQLTVGPELFYTFLVLLEETGYFNASLYSPFGIAGVET
                     LGYELAIQFREKYKKDPDIVVCTNAGGGNLTGTARGLIKAGSINTKVVGASVDLKGLH
                     MASDNQFNKKSFTTGHTGFGIPYCTWPDRSDVPRSAARPLRYMDRYVLVKQGEVFYTT
                     ELLAQLEGIERGPAGNTSLAAAFSLAQELDEDKAIVVQETEYTGAGKHICPQLTFARE
                     NGIDIKFGNPREEIAGVNLILPERPELLKCVDIDMNKIRKSFIKNCISNNHIDNIDKL
                     SNRDIEFLMEEVKSSRDFVVDVLNNL"
     gene            23013..23387
                     /locus_tag="ACOIAF_001131"
     CDS             23013..23387
                     /locus_tag="ACOIAF_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine aminomutase subunit alpha"
                     /protein_id="HGZ2668151.1"
                     /translation="MKKREDDFEVRRKHLQELSDDELKERFWSLATQIVEPMIELGKK
                     NTTPSIERSVLLRMGFSSLEVKPILEGVMERGLIGKGAGHVVYKLAKSKNITVREAGL
                     LLVKGEYWDEVTCLFREGVESC"
     gene            23381..25585
                     /gene="oraE"
                     /locus_tag="ACOIAF_001132"
     CDS             23381..25585
                     /gene="oraE"
                     /locus_tag="ACOIAF_001132"
                     /EC_number="5.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ornithine 4,5-aminomutase subunit OraE"
                     /protein_id="HGZ2668152.1"
                     /translation="MLKENKKLDIDYILKDLDKYKPKRRGWVWREHLENLEMGPFKYK
                     DCTKPLKKSVGLPSSKYFNNIDPQPSPVITTEIASGRFEDDIRRMRMAAHHGADHLMV
                     IRTAGQSHFDGLIEGTPQGIGGVPITRKQVRAQRKALDFIEEEVGRPINYHSYVSGVA
                     GPEVAVMFAEEGVNGAHQDPQYNVLYRNINMVRSFVDACEAKKIMAFANIAQIDGAHN
                     ANATAREAWKVMPELMVQHALNSIFSEKIGIDKSNICLSTVPPTAPPAPCLKIDLPYA
                     VALRELFSDYKMRAQMNTKYMESSTREATVTHVLNLLTSVLTRADIQSTITPDEGRNV
                     PWHIYNIEACDTAKQALVGMDGLMDMIELKDVGELRDKARELKERAVLYMEEISEVGG
                     YFESVEQGFFVDSGNYPERNGDGISRKIKGGVGEGTVYEREEDYLAPVTAHFGYNNVA
                     QYDENAIDNPSMLIDGCTFENPDKIIYIDELDDFDNVENRLKESYEYRNGTKIKPEME
                     WCADGIVMITMMLPTDKRTAEFAALEFVKKMNLQEIEVISREVMHESEGTRIEVKGRV
                     PFDIDLNNLVIPEEPKVLTDEEIRADIEEKPMKIVSATVGEDEHSVGLREIIDIKHGG
                     IEKYGIECHYLGTSVPVEKLVDAAIELNADAILASTIISHDDIHYKNMKKLHDYCVEK
                     GIRDKVMIACGGTQVTPEIAVEQGIDAGFGRNSKGIHVATFLVEKRREMSNK"
     gene            25585..26994
                     /locus_tag="ACOIAF_001133"
     CDS             25585..26994
                     /locus_tag="ACOIAF_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlmL-related ornithine degradation protein"
                     /protein_id="HGZ2668153.1"
                     /translation="MENSKVSLKIDVLVAEIGSTTTVVNAFHNINTNNPIFLGQGQAP
                     TTVFEGGDVRNGLSGAIKDLANKLSVDDIKYNDMFATSSAAGGLKMTVHGLVYDMTVK
                     AAKEAALGAGAVIRQITSGRIKRTDLNKIKEINPNIILIAGGVDYGERDTAIYNAEMI
                     ASMNLGIPVIYAGNVENQEEIRLIFEDTNYKLYITENVYPKIDLLNIEPTRRIIQSVF
                     EEHITTAPGMKYIKEMVNENITPTPGAVMEASKLLYKNIGDLLTLDVGGATTDVHSVT
                     DGSDYINKILVNPEPTAKRSVEGDLGVYVNMKNIVEVIGKENLQSELSIDIDAVIENY
                     PPIPKSKEEILFVERLTKEAVVKAMLRHSGKIRNIYGTTGKVKIAEGKDLTEVRYIVG
                     TGGALTRLPSRIEILDNMLKYNKNNELLFPKEKTKILIDNDYIMASMGVLSKKYEEAS
                     LKLLLKSLNFEEERLCILG"
     gene            26979..28043
                     /gene="orr"
                     /locus_tag="ACOIAF_001134"
     CDS             26979..28043
                     /gene="orr"
                     /locus_tag="ACOIAF_001134"
                     /EC_number="5.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine racemase Orr"
                     /protein_id="HGZ2668154.1"
                     /translation="MYPRLEIDIEKLKHNAKLISQMCHERNIKISFVTKSFCAQKEIV
                     EEICSEGIDHIADSRIQNLKNLQDINLPKILIRIPMLSELEEVINYCDISFNSELGTI
                     KKLNELCESKNIIHKIVLMFDLGDLREGYFYEEDFFNNVREIVRLKNIEIIGIATNLT
                     CYGAIIPSRENIGRLVSIAKRMEEKFGINLEIVSGGNSSSIHLLINNNMPEGITNLRI
                     GESILLGRETAYGENINGTYQDAFKLICQVVECKEKPSVPIGEIGVDAFRNKPVYEDK
                     GILKRAIIAIGKQDINIDSLIPIDTDIKILGASSDHMILDVSNTKYDYKLGDNLEFLL
                     TYGGIMSSSTSKYVAKKIIV"
     gene            28073..29494
                     /gene="nhaC"
                     /locus_tag="ACOIAF_001135"
     CDS             28073..29494
                     /gene="nhaC"
                     /locus_tag="ACOIAF_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HGZ2668155.1"
                     /translation="MKERECRKASLFDAMIPITCLILFLSLGVLVYGSSPHVPLIGAA
                     AVAGLVAVYRLGFKWKELELSMFNSIKMAMQAILIIIIIGVLIGTWIVSGVVPCMIYW
                     GLKILSPNIFLVASTLVCAIVSLATGSSWSTMGTVGVALLGIGQSLGMPIGLIVGSII
                     SGAYFGDKLSPLSETTNLAPAMAGTDLFTHIKYMLYSTIPSLLICLVIYGVIGMKYSG
                     QALDIKQIELIRSTLDSTFNTLSPVLLLAPAIVIGLVVFKVPAIPGLIVGAVLGVLFA
                     VVFQGESMNTILDAAQNGYVSSTQIQEVDALLSKGGIMNMMSTVSLTICALSLGGILE
                     KTGMLEVVASSLLRLAKGVFGTVLCTMVTCTITNIIAGEQYLSIVIPGRMYNKEYKKR
                     GIHPKMLSRALEDSGTLTSPLVPWNTCGAYITATLGVSALTYGPYALLNIINPIVSLV
                     LIACKFKISRIEDEPETCLNFDV"
     gene            29758..30222
                     /locus_tag="ACOIAF_001136"
     CDS             29758..30222
                     /locus_tag="ACOIAF_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HGZ2668156.1"
                     /translation="MTRISNITIAEQSEYCFLAIRKTIDFMVEFSEFSKQSFEKISKY
                     LEERGILSSGAPIVCFHNMDLVKLDVEVGFPVATYIDGKNEILQKIVPAQKIITAIDL
                     GPYELQDPTLEDLFSWANSNGYKLHGDIYYQYLNDINRPASEYLTKMMLPII"
     gene            30520..30882
                     /locus_tag="ACOIAF_001137"
     CDS             30520..30882
                     /locus_tag="ACOIAF_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGZ2668157.1"
                     /translation="MKIYDILTRFYIQDIEKAIPFYENLLKEKCSLRFSYKEVGLELA
                     QIGNVLLLSGSDDALKPFIETKSTFMVDSVDEWRSYLLDNGAVVVRDKKKVPTGYNMT
                     LRHPDGTIIEYVQHTKQD"
     gene            31477..32313
                     /locus_tag="ACOIAF_001138"
     CDS             31477..32313
                     /locus_tag="ACOIAF_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGZ2668158.1"
                     /translation="MSYKVINVITAIDYIEEHLSEKLDLDIVANAVHYSKYHLHRTFT
                     TSVGLTMHDYIKRRKLTEAAKLLVFSKKPIIEIALIAGYESQQAFTSIFKAMYKKSPN
                     KYRKEQEFYPLQLRFVLKKDNLFSKNVLELEKEIKLASMSDIPLWMNLVRLVIDGFPN
                     LQEEEYIYQLEQYILEERALILKLNNVAIANMVFNRETRSIDFFGIHPQYRNSDIAQV
                     FLKKVIEDFLIDTDISITTFREGDKADTGHRDMIKKLGFAEAELLVEFGYPTQKFILP
                     LS"
     gene            32397..34511
                     /locus_tag="ACOIAF_001139"
     CDS             32397..34511
                     /locus_tag="ACOIAF_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HGZ2668159.1"
                     /translation="MSENKQVVNCDIIASNSKHNMCEYDAELLTFFPDEIAHLIEINN
                     KLDNAFKKAENLVDKLDKDYMDAKMYMVKNRGEIDPHEMFQNEQGLKQIDNYGAFMVK
                     VRDKINKIKDSPYFARIDFRLKDMDDESKYYIGRFAFDYEDELIILDWRSPIASMFYD
                     YEIGKAGYDAPIGWVDGEITRKRQFKIKNGKLEYALESSINIQDDILQKELSHTSDEK
                     MKSIISTIQKEQNQIIRNDKADTLIIQGVAGSGKTSIALHRIAFLLYRFKDKISANNV
                     IILSPNKVFGDYISNVLPELGEEPLCELSFENIAEVQLDRVINFESEKDPLEINDAKW
                     SERVRFKSTLDFVKLIDDYIKQMPNKIFIPKDYTFGSFTAKSDWIQSRFEAYNRYPVK
                     KRLEKVAEDIHYKFESDNIMEEDLPKVKSILKSLNGMLTIKNTLTLYKDFFKQMNISN
                     MFVMATKKTLEWSDVYPFIYIHAAYEGIQEDKIIRHVVIDEMQDYTPIQYAVINLLFK
                     CKKTILGDFGQLVNPNHTHTLDDMKQLYNDGELVMLNKSYRSTFEIINFAKKIQDISS
                     LEPIERHGEEPALLKCNNEQDEINKIKIEIEEFKKSDNATLGIILKTDNDAKAVYDAL
                     REKYSVHLISSESSSFTKGVSITSIKMSKGLEFDEVIVPSVNNKTYYSDYDRSLLYIA
                     CTRAMHKLKLTYTGELTQLIDM"
     gene            35052..35990
                     /gene="blaCDD"
                     /locus_tag="ACOIAF_001140"
     CDS             35052..35990
                     /gene="blaCDD"
                     /locus_tag="ACOIAF_001140"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HGZ2668160.1"
                     /translation="MKRKKNFIWIAILLVGVVMVMYYVGKKHNDINQKTDKNYIKSEL
                     NKSKIENNNKNKKEKINMVDYSDCFEGISGGAIFYNTKNKEYNIYNKELIETRRSPCS
                     TFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYYKKLID
                     KVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQTMAKI
                     FEGDTNFKKEHINILRDIMKIDVNDKNINVYGKTGTGFDEKNKRVDAWFVGMLEREGD
                     TYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
     gene            36334..37017
                     /locus_tag="ACOIAF_001141"
     CDS             36334..37017
                     /locus_tag="ACOIAF_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2668161.1"
                     /translation="MEILKCENLTKIYGSNQTRVTALNNVNLSVQKGDFVSIVGASGS
                     GKSTLLHLLGGVDRPTSGKIYVEDTEISSLKEEALAVFRRRKVGLIYQFYNLIPTLDV
                     RKNILLPMLLDKRKVDEDRFSEIVSILGLSDRLNHLPSQLSGGQQQRVSIARSLIYRP
                     AILLADEPTGNLDRKNSEEIVDLLNLSNKRFNQTILLITHDEKIALEANRIVTMEDGV
                     IVSEKVVKK"
     gene            37014..39674
                     /locus_tag="ACOIAF_001142"
     CDS             37014..39674
                     /locus_tag="ACOIAF_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HGZ2668162.1"
                     /translation="MNILQEYTLDFVRRNKRSSIAIMLAILLTTTMMSSLCGLLYTMW
                     TDFIRLSIEKEGNWHGELFDYTYGSDLPLIENFSSVDDVMVKGQWYVGKINDEKRDYI
                     IYRNATSEYWNYMPEKDTIIEGRRPNNANEIALSKQYFENNPSVKIGDKITLPIGDRI
                     LNGKPLEPIAPKSKGESFKKISETTLTIVGKVDVSTSSVIPAYTALGYLDKTSIKPND
                     SITVYLRFKNIRDTYKELPKLAKSLGWKTNEYGKYNLKYNSNYLLKMLVFSPEQKASM
                     SSISKFSTPIMYLTIAIFTVAVFVMVIYNAFSLSANARLTQLGILSSVGASPKQIKRS
                     VVFEGFLLTIIPLPIGLFLGWLLCNRLIVYINSVNYHTDVPEVVFTYGIPAFLPAVLL
                     TIVTVWISALIPARKVSKISPIEAIRQGDSVKIKKSHKYSLGKIFGIEGELASNALRA
                     RKKSYRTATISLTLSFLLLTCFLHIIANQEGAKAVYGSDNYKDQRDIAVYLENSEPVE
                     SAFIEKLDNTEGVKSSLYYQKMSASTWLTEKDASKELAKNGGFKSIVSSKKYSPIERD
                     GKFRIISNIIGLDDKSFTEYCRQISVDPKLFYDTKNPTSIVYNKEEDINRSTRRNKVY
                     IDYLNLSIGDNIKLNEKVYDEDKGDYTYNMKVGAITSTLPKIVDTSSKYTLMQIVPMS
                     MVQEISKNYDEVSRLRSNRLIGVFKVNNREDIPRVREKLESIYNKDYGSGDYSIEDVL
                     ESEKQKASGIKTMNLIVGFITGLLAMIGLSNVLATVSGNIRSRRQEFAMLRSVGLSPE
                     GIKKMLILEGLFLGITPLLLSIPVQIGIVYAFLRINEIYFIEYLPFAPISTIIGFTIL
                     ILFIVIASYMTGYKQLKNENIVESIKNETI"
     gene            complement(39855..41036)
                     /locus_tag="ACOIAF_001143"
     CDS             complement(39855..41036)
                     /locus_tag="ACOIAF_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HGZ2668163.1"
                     /translation="MKTPRNINSTVVFIIFCLIVTFLLTVILTTITSFNEYNVLSKAL
                     GASYEYNPKSTESILKSLKNSSDKDFKTGNEILSKHGYSKSTFWSKNFFYFLVISFGI
                     ISTMATVIYIIKHRTNKQKLSRINNLTQYLEEVNLGKESVLLRCEDEFSHLEDEIYKT
                     VGELRCSKEFALKERQTLSDNLADIAHQLKTPITSMSLMAQLLSENCPDKEIDYINRL
                     TNQISRLERLVSSLLTLSKLDASTLIFESKLIDVHSLLTYAIEPIEGSLREKHQTFTI
                     EDSPSVEFEIDINWTLEALLNILKNCSEHIEDGGYIVAYYSQNPLYVEIIIEDNGKGF
                     AQEELPYIFNRFYRGKNASKDSIGIGLALSKSIIERQNGTLHAENCHNGGARFTIKFY
                     T"
     gene            complement(41036..41698)
                     /locus_tag="ACOIAF_001144"
     CDS             complement(41036..41698)
                     /locus_tag="ACOIAF_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGZ2668164.1"
                     /translation="MAKILLLEDDDTITFGIKTSLEKKNHTVCCYNTIAEAKKNFDKT
                     IDLILLDLNLPDGIGYEFCQYVKSIEDTPVIFLTVRDDEKDIIRGLDMGADDYVVKPF
                     LLSVLQSRINAVLRRTHSNTNNTITCENISLNKVEVKAYLDNVEVPLTAGEYKLLLSL
                     MENKNRAMTRTKLLEMLWDVDGNFVNDNTLTVTIKRLREKLKNPPCIKTLRGIGYRME
                     DN"
     gene            complement(41830..42468)
                     /locus_tag="ACOIAF_001145"
     CDS             complement(41830..42468)
                     /locus_tag="ACOIAF_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HGZ2668165.1"
                     /translation="MSELSKRDKEKIQRENEIIDKAEKLFCLNGFDNTTMNELAKEVE
                     YTKRTIYKYFSCKEDLFFAVVLRGYKRLWDNVKIESAKGKTGFEKIKLSYFAFHKFYS
                     VEPSLLCLMGMIGIVNSEKSDTDMLFKEKFFSFNKFMFDEIQGMFEIGKNDRSIRHDI
                     EIPTLMYSSIFTLTGFFNLLSVTGKSYLNNFNIDEEKFIETTLALLIDSIKA"
BASE COUNT        16729 a         4872 c         7675 g        13269 t
ORIGIN      
        1 agaaattgtt attttttaga ttaaatatta ttaaaaaatt atctaagttt gaaattagtt
       61 aactaacttg ctgattaatt acaatgataa catgtgtttt tgagtttagc aatgaaaact
      121 ttttttgaaa tgtcaagctt tttcataaaa aaatatataa aaaaaacttt tataagcaga
      181 gaaagaatag caaaatgcaa tggatagtgg caagatgcca cgatatgaaa aagattattc
      241 aattgcatat tgttaaaaaa cattcaaatc aagcgatatt ttgattaaat ataaagttgg
      301 cacgataaat gcttaacaat ataggtgtaa gacaataaaa caataaaaaa taattttaat
      361 aatttttaag ttattttaga taagattagg aactttgaca tcagacagtt aatatcagaa
      421 aaaagtattt tatgagtgat ttaatcgaaa ttttataatt gatactatta aggaggaaaa
      481 cgttatgctt ttagaaggag ttaaagtagt agaactttca agtttcatcg cagcaccatg
      541 ttgtgcaaaa atgttaggtg actggggtgc agaggttatt aagattgaac ctatagaagg
      601 tgatggaata agagttatgg gtggaacatt taaatctcca gcatcagatg atgaaaaccc
      661 tatgtttgaa ttagaaaatg gaaataaaaa gggtgtaagt attaatgtaa aatcaaaaga
      721 aggagtagaa atattacata aattattatc agaagcagac atatttgtaa ctaatgttag
      781 agttcaagca ttagaaaaaa tgggtatagc ttatgaccaa ataaaagata agtatccagg
      841 attaatattc tctcaaatat taggatatgg tgaaaaagga cctttaaaag ataaaccagg
      901 atttgactat actgcatact tcgcaagagg aggagttagc caatctgtta tggaaaaagg
      961 aacatctcca gcaaatacag cagcaggatt tggtgaccac tatgcaggtc tagcactagc
     1021 agcaggaagt ttagcagcat tacataaaaa agctcaaact ggtaaaggtg agagagtaac
     1081 agtaagtctt ttccatacag ctatatatgg aatgggaaca atgataacaa cagcacaata
     1141 cggaaatgaa atgcctttat caagagaaaa tccaaacagc ccattaatga ctacatataa
     1201 atgtaaagat ggaagatgga ttcaattagc tttaatacaa tacaacaagt ggttaggcaa
     1261 attctgtaag gttataaata gagaatatat attagaagac gatagatata ataacataga
     1321 ttcaatggtt aatcatgttg aagatttagt taagatagtt ggagaagcta tgttagaaaa
     1381 aacattagac gagtggtcag ctttattaga agaagcagac ttaccatttg aaaaaattca
     1441 aagctgtgaa gatttattag atgacgaaca agcttgggca aatgacttct tatttaagaa
     1501 aacatacgat agcggaaata caggtgtctt agttaatact ccagttatgt ttagaaatga
     1561 aggaattaaa gaatatacac cagcaccaaa agtaggtcaa catactgtag aagtattaaa
     1621 atctttaggc tacgatgaag agaaaataaa taactttaaa gatagtaaag ttgtaagata
     1681 ttaattagta attctagatt ttttaaaagg agaatgctaa agatgtacac aatgggatta
     1741 gatataggtt caactgcatc aaagggagta atcttaaaga atggggaaga tattgtagct
     1801 tctgaaacaa tatcctctgg tactgggact actggaccat caagagtttt agaaaaatta
     1861 tatggcaaga caggtcttgc aagagaagat attaaaaaag ttgtagttac aggatatgga
     1921 agaatgaact attcagatgc tgataagcaa ataagtgaat taagctgtca tgctagaggg
     1981 gtaaatttca taattccaga gacaagaacc attattgaca taggtggtca agatgcaaag
     2041 gtattaaaat tagataataa tggaagacta ttaaactttc ttatgaatga caaatgtgct
     2101 gcaggtacag gaagattttt agatgtaatg gcaaaaataa tagaggttga tgtatctgaa
     2161 ctcggaagta tatctatgaa ttctcaaaat gaagtatcaa taagcagtac atgtacagta
     2221 tttgcagagt ctgaggttat atcacattta tctgaaaatg caaaaattga agatatagtg
     2281 gcaggtattc atacttcagt agcaaagaga gtttctagcc tagtaaaaag aataggagta
     2341 caaagaaatg tagttatggt tggtggagtt gctagaaata gtggtattgt aagagctatg
     2401 gcaagagaaa tcaacacaga aattattgta cctgatatac ctcaattaac tggtgcttta
     2461 ggagcagcgt tatatgcttt tgatgaagca aaagaatcac aaaaagaagt gaaaaatata
     2521 taaaaagagg gatgaaaatg tctgaaaaaa aagaagctag agtagtaatt aatgatttat
     2581 tagctgaaca atatgcaaat gcatttaaag ctaaagaaga aggaagacct gtaggttggt
     2641 caacatcagt atttcctcaa gagttagcag aagtatttga cttaaacgta ttatatccag
     2701 aaaaccaagc agctggagta gcagctaaaa aaggttcttt agaattatgt gaaatagctg
     2761 aatctaaagg atattctatt gacctatgtg catatgcaag aacaaatttt ggtcttttag
     2821 aaaatggtgg atgtgaagct ttggatatgc cagctccaga tttcctactt tgctgtaaca
     2881 atatatgtaa ccaagttata aaatggtatg aaaatatttc aagagaatta gatatacctt
     2941 taataatgat tgatacaact ttcaataatg aagacgaagt tactcaatca agaatagatt
     3001 atattaaagc tcaatttgaa gaagctataa aacaactaga aattatatca ggaaagaaat
     3061 ttgaccctaa gaagtttgaa gaagtaatga aaatatcagc tgaaaacgga agactatgga
     3121 agtattctat gagtttacca gcagattctt ctccttctcc aatgaatgga tttgacttat
     3181 ttacttacat ggctgtaata gtttgtgcta gaggtaaaaa agaaactaca gaagcattta
     3241 agttacttat agaagaatta gaggacaaca tgaaaactgg taaatcttct ttcagagggg
     3301 aagaaaaata cagaataatg atggaaggta taccttgttg gccatatata ggatacaaga
     3361 tgaaaacatt agctaaattt ggagttaaca tgacaggtag tgtttaccca catgcttggg
     3421 cattacaata tgaagttaat gatttagatg gaatggcagt agcatatagt actatgttta
     3481 acaatgtaaa cctagaccgt atgacaaaat atagagttga ttctttagta gagggtaaat
     3541 gtgatggagc attctatcat atgaacagaa gctgtaaact tatgagttta atacaatatg
     3601 aaatgcaaag aagagcagct gaagaaactg gattaccata tgctggattt gatggtgacc
     3661 aagcagaccc tagagctttc actaatgctc aatttgaaac aagaattcaa ggtttagttg
     3721 aagtaatgga agaaagaaaa aaacttaata gaggtgagat ataatggaag ctattttatc
     3781 taaaatgaaa gaagtagttg aaaatccaaa tgcggctgta aaaaaatata aaagtgaaac
     3841 tggtaaaaaa gctataggtt gtttcccagt ttattgccca gaagaaatta tacatgcagc
     3901 tggaatgctt ccagttggta tatggggagg acaaacagaa ttagatttag ctaaacaata
     3961 tttccctgca tttgcatgtt caataatgca atcatgttta gaatatggat taaaaggtgc
     4021 ttatgatgaa ttatctggag ttattatacc aggtatgtgt gatacactaa tttgtttagg
     4081 acaaaactgg aaatcagcag tacctcatat aaaatatata tcattagtac acccacaaaa
     4141 tagaaaactt gaagctggtg taaaatactt aatcagtgag tacaaaggcg taaaaagaga
     4201 acttgaagaa atttgtggat atgaaataga agaagcaaaa attcatgaaa gtatagaagt
     4261 ttacaatgaa catagaaaaa ctatgagaga ctttgttgaa gtagcttata aacattctaa
     4321 tactataaaa ccatcaataa gaagcttagt aattaagagt gggttcttta tgagaaaaga
     4381 agaacatact gagctagtga aagatttaat agcaaaatta aatgctatgc cagaagaagt
     4441 ctgttctgga aagaaagttt tattaacagg tatattagct gattctaaag atatattaga
     4501 cattttagaa gacaacaata tatcagttgt agctgacgac ttagcacaag aaacaagaca
     4561 attcagaaca gatgtaccag caggtgatga tgcgttagag agattagcaa gacaatggtc
     4621 aaacatagaa ggatgttcat tagcttatga ccctaagaaa aaacgtgggt cacttatagt
     4681 agatgaagtt aaaaagaaag atatagatgg tgttatcttc tgtatgatga aattctgtga
     4741 cccagaagaa tacgattatc ctttagttag aaaagatata gaagatagtg gaatacctac
     4801 tttatatgtt gaaatcgacc aacaaactca gaataatgaa caagccagaa ctcgtattca
     4861 aacttttgct gagatgatga gtttagcgta agttcattaa aataaattta ctataaaata
     4921 tgaaaattgg aggaagctat gttatataac aaagaacaag aacttttaag aaaagcagta
     4981 agagattttg ttagtaaaga attagatact ttaccagcag aaatggataa aacaggtgta
     5041 atgcctaagg aattaatcaa aaaattagct gatgcaaaat ttataagcag taatatccca
     5101 gaagaatatg gtggtggggg agcaggatat gtttcttacg ctatagtaat ggaagaaata
     5161 gctagaagat gtgcttcaac agctactttt gttacagctg gttcttctct tgcttcatta
     5221 ccaatacttt acaatggtac tgaagagcaa aaacaaaaat acttaaaagg tatagcaaca
     5281 ggtgaattaa taggagcatt tggtttaact gagccaggag ctggttctga tgcaggtgga
     5341 caacaaacaa cagcagaatt agttggagac cactatatat taaatggtag aaaaactttc
     5401 ataactaatg gaccattctg tgatgtagct atagtaatag cagttacaga tagaagtaaa
     5461 ggacttagag gaacatcagc gtttatagta gaaagtaaat gggatggttt ctcaacagga
     5521 gctcatgaag ataagatggg tataagagga actgaaactt ctgacttaat atttgaaaac
     5581 gttaaagttc caaaagaaaa cttacttgga aaagaaggtc aaggatttaa gatagcaatg
     5641 ggtactctag aagttggtag aataggtgtt gctgccttag ctctaggaat agctcaaggt
     5701 gctttagatg aagctgttaa atatacaaaa caaagagttc aatttggtaa gcctatagct
     5761 aaattccaaa atactcaatt tactatagct gacatggaaa caaaagtttg tgcagctaga
     5821 ggattagttt atgatgcagc acaaaagaga gatgcaggaa tgagagttgc tcaagaatct
     5881 gctatggcta aatactatgc atcagaaatt gcaaatgaag ttgcttataa agcattacaa
     5941 cttcacggtg gatatggatt tataaaagat tatgaaatcg aaagaatgta cagagatgct
     6001 agaatcgtat caatatacga aggaacatca gaagttcaaa aaatggtaat ttcatcaaac
     6061 gtattaaaat aataaataat ctaatgaaat tggaggtata gactttgaaa atattagttt
     6121 gcgtaaaaca agttccagat actaacgaag taagaataaa taaagaaaca ggtactctta
     6181 taagagatgg agttccaagt atattaaacc cagatgatgc aaatgcatta gaggaagctt
     6241 taaaaattaa agatgagcat gatgatgtaa ctataacagt tataacaatg ggacctcctc
     6301 aagcagactt tatgctaaga gagtgtttag ctatgggtgc tgatgatgca atattattaa
     6361 gtgatagagc atttggagga gctgatactt gggctacatc aaatactatt gcagcgggaa
     6421 tatctaaagt aggtgactat gacataatat ttgcaggaag acaagcaata gatggagata
     6481 ctgcacaagt tggaccacaa attgcagaga aattagacat tcctcaagtt acatatgtac
     6541 aagatttcaa aatagaagga aaagatataa tagttcaaag acaattagaa gatggatacg
     6601 aattaataaa agtaagtaca cctgtacttt taacagcagt aaaagagtta aatacaccta
     6661 gatacatgtc tgttgataaa atagtaaaag catataaaca tgatgtaaaa gtttggacaa
     6721 ttgatgattt agatgtaaat aaagaagaag ttggtttaaa agcatcacca actaaagtat
     6781 ttagatcatt tactcctgaa ccaagaggaa aaggagaaat cttagaaggt aaagctgatg
     6841 aaatagctag taaaataata atcggtctaa aacaaaagca cattatataa gttggaggag
     6901 tacaaactat gaatgatata aaagatttaa gttcttataa aaacgtatgg atatttgcag
     6961 aacaaagaga aggaaaaata gctccagtag ttatagaatt attaggagaa ggaagaaaat
     7021 tagctaaaga agtagatgca gaactttgtg caatattatt aggaaaagat gttgatggat
     7081 tagctaaaga attaatcact tttggagctg acaaagttta tgttgcagat gatgctcttt
     7141 tagaaaaata tacaactgat gcatatacaa aagtaataaa agatgcaata gatgaaataa
     7201 aaccagaaat aatgcttttc ggagcaactc atataggtag agacttagca cctagaatag
     7261 cttcaagagt tggaactgga ttaacagctg actgtactaa gttagaaata gacccagaag
     7321 ataagaaaat aaaacaaact cgtccagcat ttggtggaaa cataatggct acaatcattt
     7381 gtccaaacca tagacctcaa atgtctacag ttagacctgg tgttatggat aaagctgaaa
     7441 aagacgaaac aagaactggt gaagttatag cattagacta caaaataact caagatgata
     7501 taagaactac tgttttagaa acagttaaaa ctaagaaaga tttagtatct cttacagatg
     7561 caaatgttat agtatcaggt ggtttaggat taggtggacc agaaggattt gaaatgctta
     7621 agaaattagc tgacaaatta ggtggagtag ttggttcttc tcgtgcggct gttgatgctg
     7681 gatggataga ccattctcac caagtaggtc aaacaggaac tactgttaaa ccaaacctat
     7741 atatagcttg tggtatatca ggagcaatac aacatttagc aggtatgcaa tcatcagatt
     7801 tcataattgc tataaacaaa aacccagcag ctccaatttt agaaatcgct gactatggag
     7861 tagttggaga cttacatgaa atagttccaa tgcttataga aaaattagat agtgttgatg
     7921 atttattaga agctataaaa gcttaataga tacaaactat ataaatatta ctgattgaat
     7981 aagtcttatt agttagtaac ataattatta accctttgat aagttattgc tagtgtgtat
     8041 ttgtcagtta ttaaaaagtc tgtttgaata aaattttata atacaagata atctggataa
     8101 tccttatatg tatattgctt acaaattgtg agttatattc tgtaaggatt atctttgttt
     8161 ttttaaataa atcttaaatt taattaaatt gtattaaata gacaaataat tactaaatat
     8221 gtgataaaat acaattaaaa gcaaaatgaa atttttaaca aactataatc attaaaatta
     8281 tatcttagtt acgttttaat gaaataaact acttgaaaag acgaatatta taaggagttg
     8341 attatatgtg ctgatggtat ttattgtaag caaacaataa attttgtatg tagttcaata
     8401 aattacttaa tgaaaggtgc gaatattaat ggataataat ttaatagggc ttatagaaca
     8461 tgtagaaaat ccagccattt tatgtaaaga atcaggcgaa ataatatact gtaatcatct
     8521 aatagatagt atttttagtt ttctagatat aaaaaagcct agaaatataa atgaattaga
     8581 ttcaaatttt gacaaaacag aaatactgac agatagtaaa aagaaaatag ctttcagaga
     8641 gttaaggatg actgcacata tatacaatat gaaagataat aataatgaaa ataatatagt
     8701 ttatttattt gagaagtctc taatatctga taaagtaatt gaagatataa tagagcatat
     8761 agatgaagtt gttgttgtgt ttaacaaaga tggggtaatt gaaaagatga acactgtcag
     8821 tgatgagata ttacctttta aaagaactga ggtacttggt agaaatataa cagaccttgt
     8881 gagacaagga ttagttgaag aaccaataat attaaacatg cttaaagtga agaaaaagat
     8941 ttatagaaat atcgtttacc cagatgggaa gcttattgca tatacggcag ttccaagatg
     9001 ggattctaag ggaaagctta caggaggagt actaacaggg agagatattt caagggttat
     9061 taagcttgaa tctcagataa aatatagtga tatatcagaa gacacagagt atataagtca
     9121 aagtaagatt atggacaata taaaaaaagt tgttaagaga gctgcagcct cagattcttc
     9181 tatatttata aatggagaat caggtgtagg aaaagaaata atagcaagaa caatatataa
     9241 gtatagttca aggagagaca aaccttttat agctataaac tgtggagcta taccaaatga
     9301 attattagag tcagagtttt ttggatatga agaaggttct tttactggag ctaagaaaaa
     9361 aggtaaaaag ggactttttg aagaggcgaa tggtggaact attttcttgg atgagatagg
     9421 tgagttacca atgcagatgc aaaaaaaatt acttagggtt atacaagaaa atactattac
     9481 tagaataggt ggtagtaagc ctataaaaat agatgttaga tatataagtg ctactaatat
     9541 ttctcatgaa gaccttagaa ataaccttaa atttagacaa gatttgtact atagattaag
     9601 tgttattcca gtaaaaatac ctcctcttag agaaagaaaa gaggatatag taccattagt
     9661 aaattatttc ttaaaacttt acaatgaaaa gtacaataga gaagtagaag tttcgcctaa
     9721 agttattgag ttattagaag aatattcttg gccaggaaac ataagagaat taaaaaacat
     9781 cattgaaaga tttgtagtat tatcagctaa aaatgtaatt ggagaagatg aatttaacat
     9841 gctgataaat ttagatatga ttgataatga aacagatgat ttatcaccaa tagtggtaaa
     9901 tggaattatg aatcttaatg atgcatataa aatagtggac caaataatga taagtaaggc
     9961 tataaataag tatggttcaa taacaaaggc tgctgaagtt ataggtatat atccttctac
    10021 catccataga aagataaaga gtggacatat tcacgtttag ctgtcagttt aaaaagacaa
    10081 tataattaaa atattataag atacataatt tatacaactt aaaagagatg gcttaaaaat
    10141 aaatttaatt actttaagtc atctcttttc ttttataaat atttaaaagt tatattagtt
    10201 tatagagtta gcagaaccta aaacatcatc tatcttgctt tcaactacag cttgtatagc
    10261 atctctacca gcaccaatgt attttcttgg gtcaaattct tttggatttt cagctaagaa
    10321 ttttctgata gcagcagtca tagctagtct taagtcagta tccatgttta ttttacaaac
    10381 agccatagaa gatgcttttc ttaacatatc tacaggtaca cctttagcac cagctatatt
    10441 tccaccaaat tcattacatg tagcaacagc attttggtca acagcagatg caccatgaag
    10501 aactattggg aaacctggta atttgctttg gatttcttct agtatatcga atcttaattt
    10561 agcttctcct ttgaatttga aagcaccatg agaagttcca atagctattg ctaatgaatc
    10621 aacacctgtt ctttcaacga actcaactgc ctcagctggt tgagtatact tgtgaacatc
    10681 agaagtaaca tcatcttctg ttccagctag aacaccaagt tctgcttcaa caacaacacc
    10741 tttagagtgt gcatattcaa cagcttcttt agttattcta acattttctt caaagtcaaa
    10801 atgagaacca tctatcataa ctgaagagaa accagcatca atacaagttt taatagcatc
    10861 catatttgga ccatggtcta agtgaagagc tacatctaca cctatttcat cagaagcagc
    10921 cttaaccatt tcaactaatg tatgaggtcc agcatattta acagctgaca tagaagcttg
    10981 tatcataaca taagaatttt tagctttagc agcttttaaa acaccttgta attgttctaa
    11041 gtcacttatg ttaaatgcac ctatagcaaa tccaccttcg taagcttttt taaacatttc
    11101 ttttgtagta attaatgcca ttttaaactc ctcctattaa cttttaaaat ttacccatca
    11161 cttatattat aacgttaaat caaaaaaagt acatagtaaa ttgtaataac tataaattac
    11221 aagtataatg ttaaatagaa gacaatagga ttaaataaaa taataaagaa tataaacaat
    11281 ttaggaaaat tagctatata atgctaacta taagggtagg aactacctta ttagtttgga
    11341 gatactgata acaatagttg tcttgaccaa gaagttgcca cttcaaacgt tatcgaaggg
    11401 aattaagtgg atgataattc actaaaaatt taaaataaag gagataaact atgccacaga
    11461 taaaaataag aggaattaat gaaaatgata tatgtaaaat aagtgaaaaa atgataaatg
    11521 atttagttga agctgtaaaa tgtccaagag actactttga aatagaatgt ataaagtcag
    11581 ttgcaataag agatggtaaa atagcagatg tatatccttt tgttgaagta gcttggtttg
    11641 atagaggaca agaagtacaa gatatagtag caagaataat aactgatagt ataagaaata
    11701 atttagatgt agaaagtatg gatttagcat ttacagtatt tgaaaaagaa aaatattatg
    11761 agaatggaga gcatttttaa tggctaaaaa gcaacgtata gataaaattc tttcaaattt
    11821 aggttatgga agcagaagtg aaataaagaa gtattgtaaa caagggtctg tagtagtaaa
    11881 tggaagtgaa gtttcaaatc caggaactca agtagacact gaaaatgatg agatattatt
    11941 taatggagaa gaagttatat atagagaata tatatatttg atgatgaata aacctgctgg
    12001 atatatatca gcaactacag ataaatatga cccaacagta ttagatttaa ttgatttatc
    12061 atacttagtt tttgaaccat ttcctgttgg aaggttagat aaagacacag agggattatt
    12121 agttttgact aatgatggaa agctttctca tagagtttta tctcctaaga agcatgtgcc
    12181 taagacttat tatgcaaaaa ttgatggagt agttactgaa gaagatgtag aagctttttt
    12241 agaaggtgta gtgcttgatg acggatataa aactatgcca tcacagttaa atatattaaa
    12301 aagtgatgat gaatcagaaa tagagcttac aatacatgaa ggtaagtttc accaagtaaa
    12361 aagaatgttt gaaagcgtag gcaaaaaagt agtatactta aaaagattgt ctatgggcaa
    12421 tttaaaattg gatgaaagtt tagagttagg tgagtataga gagttaacag atgaagaagt
    12481 aaaattgatt gaagaaagat aataaagata ctatagtaaa ttggaggaat gtctgtgaag
    12541 aaaaaagatg ttatagaatt tgaagtagat aaaatggaat ttggtggtac atctttaagc
    12601 caagttggag atagagttat ccatatgaag ggtggaataa gtggtcaaaa ggtgagagct
    12661 ggagttaaaa aagttagaag taagaaagct gaagttaaga tgatagaatt attagagaac
    12721 tcaccacttg aaactgaaga aacttgcaag cactttagag agtgtggagg atgtacacta
    12781 ttgtctgtgc catatgaaaa acaattagaa ataaaagaaa aacaagttat ggatttattt
    12841 ttaaagcaag atttatttgg atttcagttt ttagggatag aaggaagtcc agaaaacaaa
    12901 tattatagaa ataagatgga atatactttt ggagatgaag taaaaaatgg acctttgaca
    12961 ttaggtcttc ataaaaaagg aaagcatata gatatacaaa ctgttgagga gtgtatgttg
    13021 gttgatgaag acttctcaaa gatattagtt tctagtgtag agtttttcaa tgaaaaaaag
    13081 cttccttatt atagaacaat gaatcacaaa ggatatttga gacatcttgt agttagaaaa
    13141 gggattcaca ctaatgagat tatggttaat atagtaactt cttctcaaga agattttgat
    13201 atgtatgagt ttaaagatat gcttttaggg attgatttaa aatctgagtt ggtgggagta
    13261 cttcatacta ttaatgatgg tttagcagat gcagtccaat gtgatgagtt aagagtattg
    13321 tatggaagag attatataca agaagaaatt ttaggtctta agtttaagat ttcaccattt
    13381 tcgtttttcc aaaccaatac taagggagct gaggttcttt atagtattgc tagggatttt
    13441 attggagatt acaatgataa agttgtattt gacttgtata gtggtactgg gtctattgga
    13501 caggttatgg ctggtgctgc taagaaagtg tatggtattg agatagttga ggaagctgtt
    13561 gttgctgcta atgagaacgc taagttgaat ggacttacta attgtgagtt tattgctggg
    13621 gatgtggcta aggttgttaa ggatttaaag gacaagcctg atttgattat tgttgaccct
    13681 cctagaccag gcattcataa ggatgctatt agggatattt gtggatttgg tgctaaggag
    13741 attgtttata tttcttgtaa tcctaagtct ttggttgttg atttggttga ttttaagggg
    13801 tatggttatg agattaagat ggttaagtgt atggatatgt tcccaaatac gccacattgt
    13861 gagactgtag ttaaacttat tagagaataa cagggtttca gacttttata gtgtgtctta
    13921 aaagtagatt tgctaccata attatttttg ggtggcaaat ttattctaca atgatttcaa
    13981 aaatatttac cgtctggttt ctcatgttat tagttacatg taaatatgta tccatagttg
    14041 ttgaagtttt agcatgtccg agtatatgct ggatatcttt tatattagta ccattttcta
    14101 ataacatagt atcagggttt ttaaaataaa gtgtgtattc ttctaaagta gtgataatta
    14161 aaatatcaaa tatcactata caaaggaaag tgcacgcttt ttatgtttaa caaaaacgaa
    14221 atattaaaaa acttattgaa gaatatgatg tcaaaacaac tacagatatt caagatatgc
    14281 taaaagattt atttacaaat acaattcatc aaatatttac attgtcgaat tttaacggtt
    14341 taaacataat atattgactg atataataaa atcatagaat attttagggg gaaatagatt
    14401 atgtttaaga aaaagttaat agctgttgca atgagtataa ctatgattag tgtagggagc
    14461 ttttcgtatg ctcattcagg aagaactgat tcatcaggag gtcatcgtga taataaaaat
    14521 aaatctggat taggaagtta tcactatcat tgtggtggtt atccagctca tttacataat
    14581 aatggtggtt gtccttatac tggaggagga agcagttctg gaacaacaac ttcggttaat
    14641 aatgaagaaa agcaaaagag gtctgttgga gaaaaaggat ataatcaagg gtatgaagat
    14701 gggtataaag gaaattatag ttcatcaaat tatagtggag attatagtga tacatatgaa
    14761 tcaaagtact cagaaggata tgaaaaaggt aaggctaaat tagaagagga agaaaaggtg
    14821 gctaaagaga ctggatataa tttagggctt acaggtgcta aatctaataa tacatatgaa
    14881 aaagaagttt taaaaaatgc ttatgatacc ggatattcaa ctggatacaa tgaatataag
    14941 actaaaaaga tagaagaata taaagctaaa gggatagagg atagcaataa agataaggaa
    15001 aaaatgacat ttgaagaaaa catagattct gaatttatag atgcttataa taatgcttat
    15061 gacgaaatac aagaacaatt aaaaaatgat tacactactc aaggatttga gagtgctatc
    15121 aaaggagaaa gatttgacac atcaactata ggtaatgtta aatatgctaa ttggtttaaa
    15181 gaaggatatg ataatggtaa ggttaagtta cctaaagcaa aagaaagtgt atataatcaa
    15241 ggttacaatg aagaagattt tagtatacca gatgaaatga agtctattga aactaagtta
    15301 aaaggtgtat atgatgaagg tttagaaaaa agagaagaag agaaaagtag aaatgctact
    15361 tatggggttg gagctggaac agcagctgta gttgctggag tagtatataa gaagaaaaaa
    15421 cgtaaaaaga tatagatgct agatatacca atgatgacaa gtgtataaca aatataattt
    15481 taagtataaa tatttaaaat atagaaggga aggttatatc tatgggattt aactttagga
    15541 aaagcataaa tctaggagga ggactcaaat taaatttagg taagaagagt gtaggtataa
    15601 gtgctggtgt taaaggtgct agagtgtcag ttaattcaaa aggaagaaaa tcagctacat
    15661 tgagtatacc tggaactgga atatcatata caaaaactag tacaaagaaa cgtaaaccta
    15721 aataaatatt tgatatacta agaaatagac tgttacaaat agtctatttt ttagtatata
    15781 gatttaaaaa gtgacaaaat tttgaaatat aaactaattt cataaaggaa aatattcttt
    15841 tatgtagaat tataatattt aagtaatatt ataaaattta taggaggatt tcattatgaa
    15901 attaagcaaa acactattgg caatagggat gtctgtttca ttattgattg ctagttgtcc
    15961 aacatcaaac gctcttagtt ctattaattc aataaaaggt acaaataagt atgaaacagc
    16021 aggtttaata gcagataaac aaaattatga tattgcaata cttattaatt ctgaaaatgg
    16081 attatctgat ggattgtcag caagtggatt gtcaggagct aaaaatgccc caatattgtt
    16141 aactaagaaa gatacaatcc cttcagaaac attaaaaaga ttagaaaagg taaaagaagt
    16201 atatctaatt ggaggaaaaa attcgatagg agcatcagtt cagaaaatat taagtgataa
    16261 aaaaataagc ataaatagaa taagtggaac tgatagaata gatacaagtc ataaagttgc
    16321 aaaagaaata ggaagagtaa gtacggttaa aaaagtaata ttaactaatg cttttaaagg
    16381 cgaaccagat gctatgagtg ttgcttctat agcagttaga gataaatctc caatagtatt
    16441 aactgatgga aatactacaa gttttgttac agatggatta gaaacttatg caattggtgg
    16501 aaaagtcagt atgagtgata agttaataaa tgaaactaaa gcaacaagaa taggtggagt
    16561 agatagatac gatacaaata aacaaataat aaataaattt tataatggct caaaagaatt
    16621 ttacatagca agtgggactg atttagttta ttctttagtt ggttctacaa ttgcacaaaa
    16681 aacacctata gtgttagttg gaaatggaag taataagagt atattaaaaa atgcaagtaa
    16741 aataactgct attggaaata ttgatgatgt tgttataaaa caatgtttaa atataactaa
    16801 taatgttggg gattcaaata caggtattgt aaaagatact aattcaaata atacgaacaa
    16861 accaagtaat gagcctagct ctaataatcc tggattaact gataaaagta cagtatatgt
    16921 aactcctaat ggaaaatcat atcatctaac aaaaaattgt tctacactaa aaagaagtaa
    16981 aacaataaat aaagtaacat taaaacaagc taagtcacaa ggtaaaatag acccttgtaa
    17041 tgtttgtgta aaataatgta ttagccaaat atatattaaa aaagacaatt aattaagatt
    17101 gtctttttta ttgaagaaat ataaatttat attatgtatt attaaaaaga aaattattaa
    17161 ttataaaaaa taataataaa gacgataagt attaaaattt tatgtttcca atatttatga
    17221 tataattaat atggcaagga ataaatattt gaaaaagcta tgagtggtgt ttccatggaa
    17281 gtttcttcac tttcttatga gaggaggtga aaactatgga aaatctaatg atgagtatta
    17341 taactggtgt tatagccagt tacatttacg ataaattaaa gagccactct gagcgaccaa
    17401 ctaagagtgg ctgggaactt aatataaagt tccagaaaag taaatcttca aaataatttg
    17461 ttgaaggaaa catcactcaa agttaagtaa aatatgtttc cttgctttta ttatatcaca
    17521 aattaaaaat attaacacaa aattttgtga atatatactt attattgttc taaaaaaata
    17581 tgtaaataat tgatttgatt gcatggaatt attgttgtaa tgcttagtta aatatacaca
    17641 atctcaatta aagaagataa gtactataaa ttcatatcaa aaaaatacat aaacttttaa
    17701 gttataattt tataaaaagt cttttagtat cattatttca agaggaatta acatatgaaa
    17761 aaataacttc attagaaaag tggataaaaa actggtaaga agatgactaa agattacata
    17821 tactattact taaattaaaa atttaatata atttataatg tatacaattt attataaaaa
    17881 atttaatttt aattttctat ttttgatact ataatatata atcttgataa tttaattaaa
    17941 aatctagatt ttacatacca aactggtttc ttatttcgct atctttactt atgaactcat
    18001 atattcctat aaaggttatt ttagaaaaag ttggttattt aaacattaat accacaattt
    18061 ctatagattg tttggtgaat aatctataat tatattatgg actaaaaaat ccaatgagct
    18121 aatttattgt ttttttatat ttgagagatt acaacttatt agtttgtata tctctttttt
    18181 taataaaatc aataatatat aaaactattg gcatatttaa tgacaattca aatattgtat
    18241 ataaccataa tgaaagatat ataaataaaa ttttaaatat acttaaaaat atattaatat
    18301 aatgtacata aaaaatatca taaatatatg ataagataaa tacaatagca acattttatt
    18361 aattttttgg gtttttatat atttaggtac tggggtgata tagttgaacc aagagatatt
    18421 ttttaaaata atgaatgaaa tattggaaaa tattgatgaa ggggtacatt ttgttaataa
    18481 agacgggaaa actataatct acaataattc tgtacaaaaa atggaaaaaa tgaacagaca
    18541 cgatatttta accaattcat tttttgatat agtcaacaaa atgaaaataa acaggagtac
    18601 actattagaa gttttggaaa aaagggaagt tatcaaagac aatattcaaa aatatctgga
    18661 taaaaatgga aaagagataa cagcaattaa tactacaata ccaattgata ttaaaggcga
    18721 gtttgtaggg gcacttgaaa tatcaaagaa tatgacaacg atagaaaatt tactaaatag
    18781 aaattataaa aaaaatttag tcaataaagt tagtattaga caaaaaaagg gttatgaatt
    18841 tgatgatatt ttaggggaaa gtaatttgat taaagaagca atagctaaat ctaagaaagc
    18901 ttgcaagtct gatgcttcag tatttatata cggtgaaact ggaacgggta aagagttatt
    18961 tagccagtcc attcactatg gaagtaatag gagaaactat ccatttatag cacaaaattg
    19021 tgcagctttg ccggaatcac tattcgaagg tattcttttt ggaactagca agggaggatt
    19081 tacaggagca atagataggc caggcttatt tgaacaagct aacaaaggga cattgttatt
    19141 ggatgagata aattccatgc ctattatgtt acaggctaaa ctattaaggg ttttacaaga
    19201 aggttatgta agaagggttg gaggaactaa ggatatacct atagatgtaa gaattattgc
    19261 aacgacaaat gaaagtccaa aggtaatttt aaacgagcat aaaatgcgaa aagatttata
    19321 ttataggctt aacataattc atataaaaat tcctcctctt agagatagaa aagaggacat
    19381 acctctattg tgcaaaaaat ttatttcaaa gtataataaa aagttaaata aaaaagtggt
    19441 aggaatttca gatgaagcta ttaaaatttt aaaaaattac gattggcaag gtaacataag
    19501 agagctagaa aacataattt attgtactat gagtatgatg gaagatgagg tacaaataga
    19561 aacaagtatg attaatctaa acgactatta cactcttaaa gagaataaag agagtaacaa
    19621 ttattcttta aacgatttgg aggacaaaac acttagtaag attatcagtg agatagaaga
    19681 aaagtatata tatgaatcat tagaaaaggc atcttacaat ataactaaag ctgcatcaat
    19741 tttaggtatg aataggcaaa atttacagta taaaataaaa aaatataata tcaaaatatt
    19801 ataaataagc aagataaaaa aacaaaatca aagtaaaata aaaaagcaaa atagaggagc
    19861 aaaattatta taaaagagca aaatattttg ctcttttttt tatttctcat aagaaatatt
    19921 attttttatt tatgaaaata cataataaaa taattatagt ttcaacgttc caataaatat
    19981 ttactttaat tttaaagttg gcacgattta tgctttaact aattattgag aatatataaa
    20041 tttttaggag ggtgtatata tgagaaaagt aagagtagga atatggggat ttggagctat
    20101 gggaataggt atggctaata tgattctaaa gaaagaaggt atagaaatag tatctgtttg
    20161 tagtagaagt acttcaggga aaagtatgta tgatgtttta ggaattgaaa gaggagaaag
    20221 accagaagtt attatcaata aaaattatga agaagttttt agagaaaaaa gtgtagatgt
    20281 tgttttgtta gcaactgatt catttactaa aaaggctttt gataaaataa ttttcttatt
    20341 aaatagaaaa ataaatgtta tatcaacagc agaacaaatg gcatatccac aagctgatga
    20401 tgcagattta gctaaaaaaa tggatgaagt tgcaaaggaa aatggagtaa gtatacttgg
    20461 aacaggaata aatccaggat ttgtattaga tttactagta ttggcacttt caggtacttg
    20521 tgaagaagtt actagtataa aagctaagag agtaaatgat ttatctccat ttggaaagtc
    20581 tgttatggtg gaacaaggtg ttggagtaac aagggaagaa tttataaaag gtgtagaaga
    20641 taagactata gcaggtcatg taggttttgt agaatcaata aatatgatag ctgatggatt
    20701 aggttggaaa ttagataaga tagaacaaac aaaagagcca ataatgacta cagtagatag
    20761 aaaatctaag tacggagagg cattggctgg aaatgttgct ggttgtagac aatgtggata
    20821 tggatatgta aatggagaag ttttaattga aatggagcat ccacaacaga taataccaga
    20881 agcagaaggt attaaaacag gagactatgt atcaataaaa ggtataccaa acattgattt
    20941 acaaataaat cctgaaatac ctggaggagt aggtacttat gcaatgatag taaactctat
    21001 tccattaata ataaatgcta gaccaggtct taaaactatg ttggatatac cagtacctag
    21061 agcaataatg ggagatataa gaaatcagat agaagttgaa ttggaagaag aatcaaaggc
    21121 taattagtaa aataatagtt aattaataaa atagcagtaa actaattagt aaaataatag
    21181 tcaattaata aggggatgaa aaaatggatg ctaaaattaa tgattgggtt attatacaca
    21241 acatagtgct gactccagaa gaaagagccc cacaagttcc agaggatact aagaaagtat
    21301 ccttagaaat gtgggttaaa ggttttatac aaagtgatgc ttctataggc gacttagtag
    21361 aagttaaaac aattacaggt agacttgtaa aaggtaattt acttaaaata aatccatatt
    21421 atactcatga ttatggtaaa tgtataccag agttactcca aataggaatc caagctaaag
    21481 aaatattatt tggaggtgta tataatgaat aattcaacat caatgaaaga tatgagttat
    21541 caagctgtta tgggaagaaa taacgaaata atgaaaaatg caatcggatt ggattattct
    21601 tcttttgaac aagagggaat agggtttgat tatgagaaaa tgatgagtga aacaggatat
    21661 acattacaag atattgaagc aattcaatca cagtatgctg ttggaaatac tcctttaata
    21721 gagcttaaaa atttgactaa attagctaga aaatgtgcta aagaaggtaa aggagctaga
    21781 atatttgtaa aagatgaagc aatgaatgca tctggtagtt ttaaagcaag aagagcagct
    21841 actgcagtat atcatgccaa acaaatggga tataaaggtg taatagcagc tactagtggt
    21901 aactatggtg cagctgtagc atcacaagcg gcaatgcagg gattaaaatg tataatagtt
    21961 caagaatgtt atgattctaa tggtgtaggt caacctgaaa ttatagaaaa agcacgaaaa
    22021 tgtgaggcat atggagcaga agttgttcag ttaactgttg gaccagagtt attttatacg
    22081 ttcttagttt tattagaaga aacaggctat tttaacgcat cattatattc accatttgga
    22141 attgcaggtg tagaaacttt aggttatgag ttagctatcc aatttagaga aaagtataaa
    22201 aaagaccctg atatagttgt ttgtacaaat gcaggtggtg gtaatcttac tggaactgca
    22261 agaggattga taaaagctgg ttctattaat actaaagtag taggagcaag tgtagattta
    22321 aaaggattac acatggctag tgataatcaa tttaataaaa aatcatttac aacaggacat
    22381 acaggttttg ggataccata ctgtacatgg cctgataggt cagatgtacc taggtctgca
    22441 gctagaccac ttagatatat ggatagatat gtattagtaa aacaaggaga agttttttat
    22501 acaactgaat tattggcaca gctagaaggt atagaaagag gtccagcagg aaatacatct
    22561 ttagcagcag cattctcttt agctcaagag ctagatgagg ataaagctat agtggtacaa
    22621 gaaacagaat atacgggtgc aggtaaacat atatgtccac aacttacctt tgcaagagaa
    22681 aatggaatag atataaaatt tggaaatcca agagaagaaa ttgcaggagt aaacttaata
    22741 ttaccagaaa gaccagaatt attgaaatgt gttgatattg atatgaataa aataagaaag
    22801 tcatttataa aaaattgtat atctaataat cacattgata atattgataa gctaagcaat
    22861 cgagatatag aatttttaat ggaagaagta aaaagttcaa gagattttgt tgttgatgta
    22921 ttaaataatt tatagtaatt attgtttttt aatttaatat aaaataaaaa ttcagaaaat
    22981 tcgattaaat cgatacggat ggaggaatgt tcatgaaaaa aagagaggat gattttgaag
    23041 ttagaagaaa acatcttcaa gaactgagtg atgatgagtt aaaagaaagg ttttggagtt
    23101 tagctaccca gatagtagaa ccaatgatag aacttggtaa aaagaataca acaccatcaa
    23161 tagaaaggtc tgtcttgttg agaatgggat tttcttctct tgaagtaaaa cctattttag
    23221 aaggggttat ggaaagaggt cttataggta aaggagcagg gcatgtagtt tataaattgg
    23281 ctaaatctaa aaatataacg gttagagaag caggtttgtt attagttaag ggtgaatatt
    23341 gggatgaagt tacatgttta tttagagaag gggtagaatc atgttaaagg aaaataaaaa
    23401 attggatata gactatatat taaaagattt ggataaatat aaaccaaaac gaagaggttg
    23461 ggtttggaga gaacacttgg aaaatcttga aatgggacct tttaaatata aagactgtac
    23521 aaaaccttta aagaagagtg tagggctacc atcatctaag tatttcaata atatagaccc
    23581 tcaaccaagt ccagttatta ctactgaaat agcttctggt agatttgagg atgatataag
    23641 aagaatgaga atggcagctc atcatggagc tgaccatcta atggttatca gaactgctgg
    23701 acaatcacat tttgatgggc ttatagaagg aactccacaa ggtataggtg gagttccaat
    23761 aacaagaaaa caagtaagag ctcaaaggaa ggctcttgat tttatagaag aagaagttgg
    23821 tagacctatt aattaccatt catatgtaag tggtgttgct ggtcctgaag tagcagtaat
    23881 gtttgctgag gaaggagtca atggagcaca tcaagaccca caatataatg ttctgtatag
    23941 gaatataaac atggtaaggt cttttgtaga tgcttgtgag gcaaagaaaa taatggcttt
    24001 tgccaatata gctcaaattg atggagcaca taatgctaat gctactgcta gagaagcatg
    24061 gaaggtaatg cctgaactta tggttcaaca cgctttaaac tctatattct cagaaaaaat
    24121 aggtatagat aaaagtaata tatgcttatc aacagttcca ccaacagcac ctccagcacc
    24181 atgtttaaaa atagacttac cttacgctgt tgcacttaga gagttattta gtgattataa
    24241 gatgagagct caaatgaata ctaaatatat ggaatcatca actagagaag caacagttac
    24301 tcatgtatta aacttattaa catcagtatt aactagagct gacatacaat caactataac
    24361 acctgatgaa ggtagaaatg taccttggca tatatacaat atagaagcat gtgatacagc
    24421 aaagcaagct ttagttggta tggatgggct tatggatatg atagaactta aagatgtggg
    24481 tgaattaaga gacaaagcaa gagaacttaa agaaagagct gtattatata tggaagaaat
    24541 atctgaagtt ggtggatatt ttgaatctgt ggaacaagga ttttttgttg attctggaaa
    24601 ttatccagaa agaaatggtg atggaatatc tagaaaaata aaaggtggag ttggtgaagg
    24661 tactgtttat gaaagagaag aagattattt agcacctgta actgctcact ttggatacaa
    24721 taatgtagct caatatgatg aaaatgctat agataatcca tcaatgttaa tagatggatg
    24781 tacatttgaa aatccagaca aaataatata tattgatgag cttgacgatt ttgataatgt
    24841 agaaaataga ctaaaagaat catatgagta cagaaatgga actaaaatta agcctgaaat
    24901 ggaatggtgt gcagatggaa ttgtaatgat aacaatgatg cttcctactg ataaaagaac
    24961 agcagagttt gcagctttgg aatttgtcaa aaaaatgaac ttacaagaaa ttgaggtcat
    25021 aagtagagaa gttatgcatg aatctgaagg gactagaata gaagtgaaag gaagagttcc
    25081 gtttgatata gatttaaaca atttagtaat accagaagaa cctaaggtac taactgatga
    25141 agaaataaga gcagatatag aagaaaaacc tatgaaaata gtttcagcta cggttggaga
    25201 agatgaacat tcagttggtt taagagaaat aatagatata aaacatggag gtattgaaaa
    25261 atatggcata gaatgccact atctagggac ttctgtacct gtagaaaaat tggtagatgc
    25321 agcgattgaa ctgaatgcag atgcaatact tgcatctaca ataataagtc atgatgatat
    25381 tcactataaa aacatgaaaa aattacacga ttattgtgta gaaaaaggta taagagataa
    25441 agtaatgatt gcgtgtggag gaacacaagt aacacctgaa atagctgttg aacaaggcat
    25501 agatgctgga tttggaagaa acagcaaagg catacatgta gccactttct tagttgaaaa
    25561 aagaagagaa atgagtaata aataatggaa aatagcaaag tttctttaaa aatagatgtg
    25621 ttggtagctg aaattggaag tactactaca gtggtaaacg ctttccacaa tattaatact
    25681 aacaatccta tatttttggg tcaaggtcaa gctccaacaa cagtgtttga aggtggcgat
    25741 gtaagaaatg gcttatcagg tgctattaaa gatttagcta ataaattatc tgtagatgat
    25801 ataaagtata atgatatgtt tgcaacttct agtgcagctg gtggattaaa aatgactgtt
    25861 catggattag tctatgatat gacagtaaaa gcagctaaag aagcagcttt aggggctgga
    25921 gctgttattc gtcaaataac atcaggtaga attaaaagaa ctgatttaaa taaaataaaa
    25981 gaaataaacc caaacataat tttaatagct ggtggagtcg attatggaga aagagataca
    26041 gctatttata atgcagaaat gatagcttct atgaatctag gcataccagt tatatatgct
    26101 ggtaatgttg aaaatcaaga ggaaataagg ttaatatttg aagatactaa ttataagctt
    26161 tatataactg aaaatgtata ccctaaaata gatttattga atatagaacc tacaagaaga
    26221 atcattcaaa gtgtatttga agaacacata actacagcac caggaatgaa atacattaaa
    26281 gaaatggtta atgaaaatat tactccaacc ccaggagctg taatggaagc ttctaaactt
    26341 ttatataaaa atattggaga tttattaact ctggatgtag gtggagcaac tactgatgta
    26401 cattctgtta cagatggaag tgactatatt aataaaattt tggttaatcc tgaacctact
    26461 gcaaagagaa gtgttgaggg ggatttaggt gtatatgtaa atatgaagaa tatagtagaa
    26521 gttataggta aagaaaactt acaaagtgaa ctatctatag atattgatgc agtaatagag
    26581 aattatcctc caataccaaa atcaaaagaa gaaatattat ttgttgaaag attgacaaaa
    26641 gaagctgttg ttaaagctat gctaagacat tcaggaaaaa taagaaatat ctatggaaca
    26701 actggaaaag tcaaaattgc agaagggaaa gatttaacag aagtaagata tatagttggt
    26761 acagggggag cacttactag gctccctagt agaattgaaa tattagataa tatgctcaaa
    26821 tacaataaaa ataatgaatt actatttcca aaggaaaaaa caaagatatt aatagataat
    26881 gactatatta tggcatctat gggtgtttta tctaaaaaat atgaagaagc atctcttaag
    26941 ctcctgttaa aaagtttgaa ttttgaggag gaaagattat gtatcctagg ttagaaatag
    27001 atatagaaaa gttaaagcat aatgcaaaat taatttcgca gatgtgtcat gaaagaaata
    27061 ttaagatatc ttttgttact aaatcatttt gtgcacaaaa agaaatagta gaagaaatat
    27121 gtagtgaagg aatagaccat atagcagatt caagaataca aaacttaaaa aatttacaag
    27181 atataaatct acctaagata ttgattagaa tcccaatgtt aagtgaatta gaagaagtta
    27241 tcaattattg cgatattagt tttaactcag aattaggaac aataaagaaa ttaaatgaat
    27301 tatgtgaaag taaaaatatt attcataaga tagttcttat gtttgactta ggtgatttac
    27361 gagaaggata cttttatgaa gaagactttt ttaataatgt aagagagata gtaagattaa
    27421 aaaatattga aataattggt atagctacaa acttaacctg ttatggagct attataccat
    27481 ctagggaaaa tataggcaga ctggtaagta tagcaaaaag aatggaagaa aagtttggaa
    27541 taaatctaga aatagtatct ggaggtaatt caagttctat tcatctgttg ataaataata
    27601 atatgccaga aggtattact aatttgagaa taggcgagtc cattctactt ggtagagaaa
    27661 cggcatacgg tgaaaatata aatggaacat atcaagatgc atttaaatta atatgccaag
    27721 tagttgaatg taaagaaaaa ccatcagttc ctattggaga aataggagtt gatgccttta
    27781 gaaataaacc tgtttatgaa gataagggaa tcttaaaaag ggcaatcata gctataggta
    27841 aacaagatat aaatattgac tctttaattc caatagacac tgatataaaa atattagggg
    27901 caagttcaga ccacatgata ctagatgtaa gtaacacaaa atatgattat aaattaggtg
    27961 acaatttaga atttttatta acttatgggg gtatcatgtc atctagtacg agcaaatatg
    28021 ttgcaaaaaa aattatagtg tagtgtttaa ataattagga ggaaactatt ttatgaaaga
    28081 gcgagaatgt agaaaagcaa gtctgtttga tgcaatgata ccaattactt gtttgatatt
    28141 atttttaagt ctaggagtat tagtgtatgg ttcatcacct catgtaccac taataggagc
    28201 agcagcagta gcaggacttg tggctgtata tagacttgga tttaaatgga aagagttaga
    28261 attaagtatg tttaatagta taaagatggc aatgcaagca atcttaatca taattataat
    28321 aggcgtactt ataggaactt ggatagtaag tggagtagtt ccatgtatga tttactgggg
    28381 actaaaaatt ttatcaccaa atattttttt agtagcatca acattagtct gtgcaatagt
    28441 atctcttgcg acaggttcat cttggagcac aatgggtaca gtaggagtag ctcttttagg
    28501 aattggtcaa agtttaggta tgccaattgg attaatcgta ggttctataa tatctggagc
    28561 ttactttgga gataaactat cacctttatc tgaaacgact aacttagctc cagcaatggc
    28621 aggaacagat ttgtttacac atataaaata tatgctatat tcgactatac catcattatt
    28681 aatttgttta gtaatttatg gtgtaattgg aatgaagtat tcaggtcaag cactagatat
    28741 aaaacaaata gaattaatta ggtctacatt agattctaca tttaatactt tatcaccagt
    28801 attattatta gctccagcaa tagtaattgg attagttgta tttaaagttc ccgctatacc
    28861 agggcttata gtaggtgctg tattgggagt attgtttgca gtagtttttc aaggagaatc
    28921 tatgaatact atattagatg cagcacaaaa tggatatgtt tcgtcaacac aaatacagga
    28981 ggtagatgct ttattatcta aaggtgggat tatgaatatg atgtccactg tatccttaac
    29041 aatatgcgct ttatctcttg gaggtatttt agaaaaaaca ggtatgcttg aagtagtagc
    29101 atcttcttta ctaagattag caaagggtgt atttggaaca gtattatgta ctatggtaac
    29161 ttgtacaatt accaatataa tagcaggtga acaatatcta tctatagtta taccaggaag
    29221 aatgtataat aaggaatata aaaagagagg tatacatcct aagatgttgt caagagcttt
    29281 agaagatagt ggaactttaa cttctccatt agtaccatgg aatacatgcg gggcgtatat
    29341 aacagcaaca ttaggtgtat ctgcactaac atatggtcca tacgctttat taaatattat
    29401 aaatccaata gtatctttag tattaattgc atgtaagttt aaaatatcaa gaatagaaga
    29461 tgaacctgaa acttgtttaa attttgatgt ttgatatttt aattgtttgt ataaattttt
    29521 tatagaaaaa gatttctttt agacttgcct aaaaattagg caagtctttt aagtatgttc
    29581 tgattataag agcactgtgt gacaagaatg atgagattat ttttgctata ctttcgatat
    29641 aagaaatgga aggaggttat gttttatatt catatgaaat aattagttac acagtgaatt
    29701 ttcgtataat ttggtataaa aagattagaa atgaaaataa agtaatggag gaataaaatg
    29761 actagaatat ccaatataac aatagctgaa caatctgagt actgtttttt ggcaatacga
    29821 aaaacaattg actttatggt agaattttca gagttttcaa aacaaagttt tgaaaaaatt
    29881 tctaaatatc tggaggagag aggaatttta tcttcaggag caccgattgt atgttttcat
    29941 aacatggatt tagtaaaatt ggatgttgaa gtagggtttc ctgtagccac ttatatagat
    30001 gggaaaaatg agattctaca aaagattgtt cctgcacaga aaataataac tgcaattgat
    30061 ttaggacctt atgaattaca agaccctact ttagaagact tattttcatg ggcaaatagc
    30121 aatggatata aacttcatgg cgatatatat taccagtatt taaatgatat caatcgtcca
    30181 gctagtgaat atcttacgaa aatgatgtta cccattatat agaaagttaa agattatcgt
    30241 ttgttagttg ataattagaa gaacatttac tgctactgca taaattttat cctaaataat
    30301 atcaaggtga tttaatttat gtattctaat gtaactgtag ataatcaatt tataactaac
    30361 ttagtgtaat ttagatttaa tttattattc taatgtaacc ccaatatatt gttaaggtct
    30421 acttaagatg ctcaaaaggc tatgcttaat ttattgttat ataatttgta gagtggtata
    30481 atttagaggt aatattatat agatatatag ggggaaaata tgaaaatata tgatatatta
    30541 acacgttttt atattcaaga tatagaaaaa gccatacctt tttatgagaa tcttttaaaa
    30601 gaaaaatgtt ctttgcgttt ttcctataaa gaagttgggt tggaattagc gcaaatagga
    30661 aatgttttac tattatctgg ttctgatgat gctcttaaac catttataga aacaaaatct
    30721 acttttatgg ttgattctgt tgatgaatgg agaagttatc tattggataa tggagcagtt
    30781 gtagtaagag ataagaaaaa agttcctaca ggatataata tgacattgag acatccagat
    30841 ggtacaatta tagaatatgt tcaacataca aagcaggatt agttaaaaac agattttact
    30901 actgagcaac taattagaat ctattcttag acgtacaatt tattattttt ggtattaaca
    30961 gattataagt caaattataa tatttcaaaa tagaatagaa atttatttac ataaaagaga
    31021 gtgtttaaaa atgaactttt tagttcatga agcactcttt tttgtatgaa atcaaataca
    31081 tcaatatata aaataaactg atgaagagct taaattaaaa taaaaagatt cataacagga
    31141 ccccattaaa ctttaagata gggtaagttt gtaatcatat taaataattg taaaaagctg
    31201 tttttagtcg tataatataa atatttatca tttaattaag gaatctaaaa ggaatctaat
    31261 aagaagaaaa taataaataa ctttttagat ttactaaatg aaaagttatt gaaaattaaa
    31321 ggattagagt ctttaatcag aaagtaaaaa gtaatatatt aaatttatga attgaaaact
    31381 taaacaacaa atttgtttat aaaatagcaa gattcattaa atataactaa gtaataattg
    31441 atataatgtt gcatggctaa aaggagggaa gctgcaatgt cttacaaagt aattaatgtc
    31501 ataactgcta ttgactatat agaggagcat ttatcagaaa agctggattt agatatagtt
    31561 gctaatgctg ttcattactc aaaatatcac ctgcatagaa ctttcacaac ttctgttggc
    31621 ctaacaatgc atgattacat aaagcggaga aagttgacag aagcagcaaa attattggta
    31681 ttttcaaaga aaccaattat tgaaattgca ttgatagctg gatatgaaag tcaacaggca
    31741 ttcacttcta tatttaaagc tatgtataaa aaatctccaa ataaatatag gaaagaacaa
    31801 gaattttatc ctttgcagct aagatttgtt ttgaaaaaag ataacctatt ttcaaagaat
    31861 gtactagagt tggaaaaaga aattaaactt gcaagtatgt ctgacatacc attatggatg
    31921 aatttagtga gattagtcat agatggattt cccaatttgc aagaggaaga atatatatat
    31981 caattagaac agtatatttt agaagaaaga gctttaatat taaaacttaa taatgtcgct
    32041 attgcaaata tggtgtttaa tagagaaact agaagtattg atttttttgg aatacatcca
    32101 cagtatcgta attcagacat tgcacaagtg tttttgaaga aagttattga agatttcttg
    32161 atagatacag atatttcaat tactaccttt cgagagggtg acaaagctga tacaggacat
    32221 agagatatga taaagaagtt ggggtttgca gaagcagagt tgcttgtgga atttggatat
    32281 cctactcaaa aatttattct tcctttaagt taaatagcaa gaatgattaa aaactccaaa
    32341 acacaattaa gtataatgat tcagtgtaag tacttaatta acaaaggagg atttaaatgt
    32401 ctgaaaataa acaagtagta aattgcgata ttattgcatc taattctaaa cataatatgt
    32461 gtgaatatga tgcagagtta ttaacttttt ttccagatga gattgcacat ctaatagaga
    32521 taaataacaa gctagacaat gcttttaaga aagctgaaaa tcttgttgac aagcttgata
    32581 aggattatat ggatgctaaa atgtatatgg taaaaaatcg tggtgaaata gacccacatg
    32641 aaatgtttca aaatgaacaa ggactaaaac aaatagataa ctatggggct tttatggtaa
    32701 aagttcgaga taagattaat aaaatcaaag attctccata ctttgctaga atagatttta
    32761 ggctgaaaga tatggatgat gaatctaagt attatattgg tagatttgca tttgattatg
    32821 aagatgagct tataatactt gactggcgtt ctccaattgc cagtatgttt tatgattatg
    32881 aaatcggaaa agctggatat gatgctccaa taggatgggt ggatggagaa ataacaagaa
    32941 aacgacaatt taaaattaaa aatggaaaat tagaatatgc actagaaagt tccattaata
    33001 tacaagatga tattcttcaa aaagaactta gtcacacttc agatgaaaaa atgaagtcaa
    33061 tcatttcaac aatacaaaag gaacaaaatc aaattattag aaatgataaa gctgatacac
    33121 tgattattca aggggtagct ggttctggga aaacatctat agcattacac agaattgctt
    33181 ttcttcttta tcgatttaaa gataaaatat cagcaaataa tgtaattatc ctatcaccta
    33241 ataaagtctt tggagactat atttccaatg tccttcctga gcttggagaa gagccattat
    33301 gtgaattaag ttttgaaaat atagcagagg tacaattaga tagagttatt aattttgaaa
    33361 gtgagaaaga cccattagaa ataaatgatg ctaaatggtc tgaaagagtg cgttttaaat
    33421 ctacacttga ttttgtaaaa cttattgatg attatataaa acaaatgcca aataagattt
    33481 ttattccaaa agactacact tttggaagtt ttacagctaa aagtgactgg atacagagta
    33541 gatttgaggc atataacaga tatccagtta agaagagatt agagaaggta gctgaagaca
    33601 ttcattataa atttgaatca gataatatta tggaagaaga tttaccaaag gtaaaaagta
    33661 tacttaaaag tttgaatgga atgttgacaa taaaaaatac tcttacacta tataaggatt
    33721 tctttaaaca aatgaatata tcaaacatgt ttgtgatggc aacaaaaaaa actcttgaat
    33781 ggtcagatgt atacccattt atatatattc atgctgcata tgaaggtata caagaagata
    33841 agattataag acatgttgtt attgatgaaa tgcaggatta cacacctatt caatatgctg
    33901 taataaactt actttttaag tgtaaaaaga ctattcttgg agattttgga caattggtta
    33961 atcctaatca cactcataca ctagatgata tgaaacagct atataatgat ggggaactag
    34021 ttatgttgaa taagagttac cgttctactt ttgagattat taatttcgcc aaaaagattc
    34081 aagacatatc ttcattagag cctattgaac gacatggtga agaaccagct ttattaaaat
    34141 gtaataacga gcaagatgaa ataaataaga taaaaataga aattgaagaa tttaaaaaga
    34201 gtgataatgc gactcttggt attatattaa agacagataa tgacgctaaa gctgtatatg
    34261 atgcgttaag agaaaaatat agtgttcact taatatcttc tgaaagttct agctttacaa
    34321 aaggagtatc cataacttct attaaaatgt caaaaggttt agagtttgat gaagttattg
    34381 taccttcagt aaataataaa acatattata gtgattatga ccgcagtttg ttatatattg
    34441 cttgtacacg agctatgcat aaacttaagt tgacatatac tggtgaattg actcaactta
    34501 tagatatgta gattatatac ttattatgta agtgtttact tatataaata taacctgtca
    34561 ataaaattca ttaataaatc aataaaaaca atatttgtaa aagtaaaagc tataaattat
    34621 atattaaatt ttataattta tagcttttat aatactgctt atcttaatcc tcccaatagc
    34681 aaaataattt taaacatgcc atattttata aatgtaataa caattataca attaaaataa
    34741 ctttcccaaa ctaacttgac atttaaaact tactattgta atatttaaat attacaatag
    34801 taagttttaa atgtggaatc aattattata tcacaaatta aaaggaggac aggatgttat
    34861 taatagttac ctaattatct tcgcaatagg tttaatcaac ataattgtac taggtgaaga
    34921 aaatataaga gatacagcta ttcatttagt cttgtttctt ataagtagaa cctagattgg
    34981 agtatattac aaataatgtc tactatttta atagtaggtt ataaatttaa atatgggaga
    35041 tgatataaat tatgaaaaga aaaaagaatt ttatttggat agctatctta ttggttggtg
    35101 tagttatggt aatgtattat gtagggaaaa aacataacga tataaatcaa aaaactgata
    35161 agaattatat aaaatctgaa ttaaataaat ccaaaattga aaacaataat aaaaacaaaa
    35221 aagaaaaaat taatatggtt gactatagtg attgttttga aggtattagt ggaggagcta
    35281 ttttttataa tactaaaaat aaagaatata atatatataa caaagaatta attgaaacaa
    35341 gaaggtcacc atgttctact tttaaaatag tttcaacttt aattggttta gaaaaagggg
    35401 taataaactc aaaagaatca gttatgggat atgatggtac agagtatcca aataaaaatt
    35461 ggaataaaaa tcttagttta gaagaggcat ttaaggagtc ttgtgtttgg tactacaaaa
    35521 aattgataga taaagttgat gctaaatctg tccaaaatat tttagatgat ttaaagtatg
    35581 ggaattgcga tattagcgaa tgggaaggag atttaaaaaa tggaaaggga catttaaatg
    35641 gattttggtt agaatcttca ttacaaatat ctccaaaaga acaagtgcaa acaatggcaa
    35701 aaatatttga gggagataca aactttaaaa aagagcatat taatatttta agagatatta
    35761 tgaaaataga tgtaaatgat aaaaacataa atgtatatgg aaaaactgga acaggatttg
    35821 atgaaaaaaa taaacgtgtg gatgcatggt ttgttggtat gctagagcgt gaaggtgaca
    35881 cctattattt tgctattaaa tcagatgatt ccaataaaga aattacaggg ccaaaggtaa
    35941 aggaaattgc aattaatata attaagaaat attatagtgt aagggaataa acttaatttt
    36001 atttgtaaat attatttacc tatactctgt aatatttatt aaataatttt actaaattga
    36061 gtctatatag agcaaaactc ctttagtaca taaaatactt gctattgatt attaaattaa
    36121 atacctatag atttaaagtt aatacctttt aaagactatg taatatatta aaagtataaa
    36181 gaaaatatat tgcatagttt ttgttttata ttataatttt aaataatgtg caaaaactaa
    36241 acaatatttt taatagtaat ttatatttgt attcatgtca ctataatgtc attttaggtt
    36301 gctaaaattt aaccataaag gagatgattg tatatggaaa tattaaaatg tgaaaatctc
    36361 acaaaaatat atggttcaaa tcaaaccaga gtgacagccc ttaataatgt aaatttatca
    36421 gttcaaaaag gtgattttgt atccattgta ggagcatctg gttctggcaa atctacattg
    36481 ttacatctgc ttgggggagt agatagacct acatctggaa agatttatgt tgaagataca
    36541 gaaatatcaa gtttaaagga agaagctctt gcagttttta gacgtagaaa agtaggtctt
    36601 atttatcaat tttataatct tatacctacc cttgatgtta gaaaaaatat attattaccc
    36661 atgttacttg ataaaagaaa ggtggatgag gatagattct ctgagattgt atctatattg
    36721 ggattatctg atagattaaa ccatcttcca agtcaacttt caggtggaca acaacaaagg
    36781 gtatctatag ctagaagttt aatctataga ccagctattt tattagcaga tgagccaact
    36841 ggtaatcttg atagaaaaaa ttcagaagaa attgtagact tgttaaactt atcaaataaa
    36901 cgatttaatc agacaatact tcttattact catgatgaga aaatagcact agaggcaaat
    36961 cgtattgtaa ctatggaaga tggagtgatt gtctctgaaa aggtggtgaa gaaatgaata
    37021 ttcttcaaga atacacctta gactttgtac gacgcaataa acgtagtagt atagctataa
    37081 tgttagctat attacttact acaaccatga tgtcttcttt gtgtggactg ttgtatacca
    37141 tgtggacaga ttttataaga ctttcaattg agaaagaagg taactggcat ggtgaattgt
    37201 ttgactatac ttatggaagt gacttaccat taattgaaaa tttttcttca gttgatgatg
    37261 taatggtaaa aggacaatgg tatgtaggca aaattaatga tgagaaaaga gattatataa
    37321 tttatagaaa tgctacatca gagtattgga attatatgcc agaaaaggat actataatag
    37381 agggtcgtag accaaataat gctaatgaaa ttgcattatc aaaacaatat tttgagaata
    37441 atccaagtgt aaaaattggt gataaaatta ctctcccaat aggagataga atcctaaatg
    37501 gaaagcctct tgaacccata gccccaaaat cgaaagggga aagttttaaa aaaatttcag
    37561 agactacatt gacaatagta ggaaaggtag atgtttctac ttcatcagta atacctgcat
    37621 atacagctct tggctatctt gataaaacaa gtataaaacc taatgatagt ataactgtgt
    37681 acttgcgatt taaaaatata agagacacgt ataaggaact tccaaaactt gctaaatcat
    37741 tgggatggaa gactaatgaa tatggaaaat acaatcttaa atacaatagt aattatcttt
    37801 taaagatgtt agtcttttca ccagaacaga aagcatctat gagtagtata tcaaaatttt
    37861 caactcctat tatgtatctt acaatagcaa tttttacagt agccgtgttt gtaatggtga
    37921 tttacaacgc gttttcactg tctgcaaatg ctagacttac tcagttaggg atattatcta
    37981 gtgttggtgc atcaccaaaa caaattaaaa ggtctgtagt gtttgaaggc tttttactga
    38041 caattatacc attaccaata ggtttatttt taggatggtt attgtgtaat aggcttatag
    38101 tttatattaa ttcagttaat tatcatactg atgttccaga agtggtattt acttatggta
    38161 ttcctgcttt tctgccagca gttttactta caatagtaac agtctggatt tctgcactta
    38221 taccagcacg aaaagtatca aagatatctc ctatagaagc aatacgacaa ggagatagtg
    38281 ttaaaatcaa aaagtcgcat aaatattcat taggaaagat atttggaata gaaggagaac
    38341 ttgcatcaaa cgcactacgt gctcgtaaaa aatcttatcg tactgcaaca atatctttaa
    38401 cattatcttt tttattactt acatgttttt tgcatataat tgcaaatcaa gaaggagcaa
    38461 aagctgttta tggaagtgat aattataagg accaaagaga tatagcagtt tatttggaaa
    38521 atagtgaacc tgtagagtct gctttcattg aaaaattaga taatacagaa ggtgtaaaaa
    38581 gttcactata ttatcaaaaa atgtctgctt ccacttggct gactgaaaaa gatgcatcaa
    38641 aagaacttgc gaaaaatgga ggatttaaaa gtatagtttc atctaaaaaa tactctccta
    38701 tagaacgtga tggcaagttt cgtataattt caaatataat tgggcttgat gataagagtt
    38761 ttacagaata ttgtaggcaa atttctgtag acccaaagtt attttatgat acaaaaaatc
    38821 caacctcaat tgtatataat aaggaggaag atattaatcg tagtacacgt agaaataaag
    38881 tatatataga ttatttaaat ctttcaatag gagataacat taaattaaat gaaaaagtat
    38941 atgacgaaga taaaggggat tatacttaca acatgaaagt gggagctatt acaagcacac
    39001 ttcctaaaat agtagatact agttcaaaat atacattaat gcaaatagtg ccaatgtcta
    39061 tggtgcagga aatatctaaa aattatgatg aagtaagtag gttacgttca aataggctga
    39121 taggagtatt taaagtaaat aatcgtgagg acattcctag agtaagagaa aaattagagt
    39181 ccatttataa taaagactat ggttcaggag attatagtat tgaggatgtt ttagaaagtg
    39241 aaaaacaaaa ggctagtgga ataaagacca tgaatttaat agtaggcttt attacaggtt
    39301 tattggctat gattggatta tcaaatgtac ttgctacagt gtcaggaaat attagaagta
    39361 gacgacaaga gtttgctatg ttgcgttctg ttggattatc tccagaaggc ataaaaaaga
    39421 tgcttatttt ggaaggttta ttcttaggaa ttacaccatt actacttagt attcctgtgc
    39481 aaataggaat agtgtatgct tttctacgta taaatgaaat atattttata gagtatttac
    39541 catttgcacc tatatctaca ataataggtt ttactatatt gatacttttt attgtaatag
    39601 catcatatat gacaggatac aagcagttga agaatgaaaa tatagtagaa tctataaaaa
    39661 atgaaacaat ataaacaaaa tctatttgaa cttatttaaa ggtttcgatt taagactact
    39721 tacaagtata gggatagtta agttgtttgt ttaataaaac cttaaatgta aatagaagta
    39781 tattactaaa acaatagata acatagaata tagatatagc tggattatag gtattttacc
    39841 tataatccag cattttatgt gtaaaattta attgtgaatc ttgcaccacc attatggcaa
    39901 ttttctgcat gtagagtacc attttgacgt tctataatag actttgatag agcaagccct
    39961 ataccaatac tgtccttact agcattttta ccacgataaa atctattaaa aatatatgga
    40021 agttcttcct gagcaaaccc cttaccatta tcttctatga taatctcaac atatagagga
    40081 ttttgactat aataagcaac tatatatcca ccatcttcta tatgctcact acaattttta
    40141 agaatattta aaagagcttc taaagtccaa ttaatatcga tttcaaattc taccgaagga
    40201 gaatcctcga tagtaaatgt ctgatgtttt tctcttaaag aaccttctat aggctcaatt
    40261 gcataagtaa gaagagaatg tacatctata agtttgcttt caaatattaa agtacttgca
    40321 tccaattttg ataatgtaag taaagatgaa actagtctct caagtcttga gatttgattt
    40381 gtaagtctgt taatataatc tatttcttta tctggacaat tttctgaaag taattgtgcc
    40441 ataagtgaca tagaagtaat tggagtctta agttgatgag caatatctgc aagattatct
    40501 gaaagagttt gtctttcttt taaagcaaat tcttttgaac aacgaagttc cccaactgtt
    40561 ttatatatct catcttcaag gtgagaaaat tcatcttcac atcgtaaaag tacagactct
    40621 tttcctaaat tcacttcttc taagtattga gtaagattgt ttattctact cagtttttgc
    40681 ttatttgttc tatgtttgat tatatatata actgtagcca ttgttgaaat tataccaaac
    40741 gatatcacta gaaaataaaa aaagttttta ctccaaaatg tacttttaga atatccatgt
    40801 tttgataaaa tttcatttcc tgttttaaaa tccttatcac tagaattttt cagtgatttt
    40861 agtatacttt ctgtagactt aggattgtac tcatatgatg caccaagagc cttagacagt
    40921 acattgtatt cattaaaaga agttatagtt gttagaatta cagtcaataa aaaagttaca
    40981 attaagcaga atattataaa aacaactgta ctgttgatat ttcttggagt tttcactagt
    41041 tgtcctccat tctatatcct atgcctctta atgtttttat acaaggtgga ttttttaatt
    41101 tttctctaag acgttttatt gtaacagtaa gagtgttatc atttacgaaa ttaccatcta
    41161 catcccagag catttctagt aactttgtac gagtcatagc tctattttta ttttccataa
    41221 gacttagaag aagtttatat tctcctgctg tcaaaggtac ttcaacatta tctaaatatg
    41281 ctttgacttc aaccttattt aagcttatat tttcacatgt aatagtattg ttagtatttg
    41341 aatgagttct tctaagaact gcattaattc tggattgaag tactgagagc aaaaacggtt
    41401 taaccacata gtcatctgca cccatatcta atccacgtat aatgtctttt tcatcatcac
    41461 ggactgtcaa aaatatcact ggagtatctt ctatactttt aacatattgg caaaactcgt
    41521 atccaatacc atcaggtaga tttaaatcaa gcagaattaa atcaatagtt ttatcaaaat
    41581 tttttttagc ttcagcaatc gtattataac aacatacagt gtgatttttc ttttcaaggg
    41641 aagtttttat tccaaatgta attgtatcat catcttctaa aagaagtatt ttagccattt
    41701 acttttaatc ctttcagtaa tttagttatt ctattaacta agttattagt tttatgttta
    41761 atatttttta tattagagat agaaaaaatt atcatttaag tatataaact ttaatttata
    41821 aacttaggtt tatgctttaa ttgaatctat taaaagagca agagttgttt caataaattt
    41881 ttcctcatct atattaaagt tgttcaggta tgattttcct gttacagata gtaaattaaa
    41941 aaatcctgta agtgtaaata ttgatgaata cataagagta ggtatttcaa tatcatgcct
    42001 aatacttcta tcattttttc caatttcaaa cattccttgt atttcatcaa acataaattt
    42061 gttaaaagaa aaaaactttt ctttaaacag catatcagta tccgattttt cagagtttac
    42121 aataccaatc attcccataa ggcataatag agaaggttct acagagtaaa atttatgaaa
    42181 agcaaagtat gaaagtttta ttttttcaaa ccctgttttg ccttttgcac tttctatttt
    42241 aacattatcc caaagtcttt tatatcctct taagacaaca gcaaagaata aatcttcttt
    42301 acatgaaaag tacttgtata tggtgcgttt agtatattca acttcttttg ctaattcatt
    42361 catagttgta ttatcaaatc cattaagaca aaataatttt tctgccttat caataatctc
    42421 attttctctt tgtatttttt ctttatctct ttttgataat tctgacatat aaattattcc
    42481 tttctattga taaaaaagta tactgatggt atacttatgt aaatataata tttgtatacc
    42541 tatag
//
LOCUS       DBGJFF010000021        42230 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.021, whole genome shotgun sequence.
ACCESSION   DBGJFF010000021 DBGJFF010000000
VERSION     DBGJFF010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 42230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42230
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.021"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            <1..577
                     /locus_tag="ACOIAF_001146"
     CDS             <1..577
                     /locus_tag="ACOIAF_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HGZ2668166.1"
                     /translation="NVYHTKEEMIDCVNAFYEGMVQRSEEMKRHPNYKTGENYAYLGL
                     PPCFLIFDEYVAFFEMLGTKESVGLLSQLKKIVMLGRQAGYFLIVACQRPDAKYFSDG
                     IRDNFNFRVGLGRISELGYGMLFGSDVKKQFFQKRIKGRGYCDVGTSVISEFYTPLVP
                     KGHDFLQTIGSLAQARQDGTATCEAKGDGTD"
     gene            760..1956
                     /gene="mobT"
                     /locus_tag="ACOIAF_001147"
     CDS             760..1956
                     /gene="mobT"
                     /locus_tag="ACOIAF_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HGZ2668167.1"
                     /translation="MVLNEEQWIKELREKRIAYGISQGRLAVASDITREYLNKIESGK
                     MKPSKELLETLHKELARFNPEAPLTMLFDYVKIRFPTLDIQHIIKDILKLNINYMLHE
                     DYGHYSYTEHYSLGDIFIYTSADEEKGVLLELKGRGCRQFESYLLAQQRSWYDFLMDA
                     LVDGGVMKRIDLAINDHTGILDIPELAEKCRKREYIGKSRSYKFYQSGELIKHREDDR
                     EYMGRTLYLGSLKSDVYFCIYEKDYEQYVKLGTPLEKAEIINRFEIRLRNERAYYAVR
                     DLLTYYDAEQTAFSIINQYVRFVDEEPDKRKNDWKLNERWAWFIGDNRQSLKLTTKPE
                     PYTLDRTLRWVQRQVAPTLKMLKRIDKGNGTDYMKTIEQQAKLTEKHEMIIKQQTTPA
                     KDLVES"
     gene            1970..2104
                     /locus_tag="ACOIAF_001148"
     CDS             1970..2104
                     /locus_tag="ACOIAF_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HGZ2668168.1"
                     /translation="MWELLKDFLLVSVGTGIGVALMCILSVGKEADYEMKKLKESEDK
                     "
     gene            2106..2327
                     /locus_tag="ACOIAF_001149"
     CDS             2106..2327
                     /locus_tag="ACOIAF_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668169.1"
                     /translation="MNFGQNLYQWFLSNAQSLVLMAIVVIGIYLGFKREFSKLIGFLV
                     VALIAVGLVFNAGGVKDVLLELFNKIIGA"
     gene            2362..3003
                     /locus_tag="ACOIAF_001150"
     CDS             2362..3003
                     /locus_tag="ACOIAF_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGZ2668170.1"
                     /translation="MWIYKILIYGGILLKKAMIYVHGKGGSYLEAEQFKNICLDFDVF
                     GVDYNDYFPWIVKNEIMEVYDKISEKYDYIYVLANSIGAYFTMHTLQNFKIEKALFIS
                     PILDMEQLIVDMMSWANVSEKELSERKEIPTDFGETLSWEYLCFVRENPINWKTPTEI
                     LYAEHDNLTSRQTVDKFIDRHCANLTVMNDGEHWFHTNEQIFFRDNWLRKVIQ"
     gene            3092..3388
                     /locus_tag="ACOIAF_001151"
     CDS             3092..3388
                     /locus_tag="ACOIAF_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005339688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HGZ2668171.1"
                     /translation="MSDIFKDMQANVGCDYISNLPSYKRKVWHEMKRLPLTNYDIKQL
                     EDFSKYVFGMSYQSLKDVMKQQKGREEQCRKQGCWWKREEQLAKKQHHTGSTYR"
     gene            3307..3810
                     /locus_tag="ACOIAF_001152"
     CDS             3307..3810
                     /locus_tag="ACOIAF_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HGZ2668172.1"
                     /translation="MQETRVLVETRGTTGKETTSYWFDLPIDVAEFEEKLGVGAESQD
                     YRIIEKVLPYADEVHEQTSVYQLNELDFMYRQLPADMQEEYIALLTVFENLEALYICR
                     NVITVYPDCKSMIDVAKQKLMNDPTFKHLSEDCQAYYFDFEAYASHLLENRRYLVTEH
                     SVFELPA"
     gene            3828..4331
                     /locus_tag="ACOIAF_001153"
     CDS             3828..4331
                     /locus_tag="ACOIAF_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HGZ2668173.1"
                     /translation="MIDDMAVYIANLGKYNEVYLVGAWFTFPIDEEDVKEKIGLNEQY
                     EEYAIHDTDNFPIAIGEYVSIDELNEMYEMLEELPDYIVECLDEFIGHYGTLEEVVEH
                     KDDIYYYPDCETMTDVAYYYIDELQALGDIPPSLQNYIDYEAYGRDLDIGGCFIETSR
                     GMCEIPY"
     gene            4449..4847
                     /locus_tag="ACOIAF_001154"
     CDS             4449..4847
                     /locus_tag="ACOIAF_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HGZ2668174.1"
                     /translation="MKKIKSYTGIWNVEKVLYAINDFNLPFPVTFTQITWFVITEFII
                     ILFGDIPPLSMIEGAFLKYFGIPVALTWFMSQKTFDGKKPYSFLKSQITYALRPKITY
                     AGKAVKLHKQILNETITAVRSVNYVPDKIY"
     gene            4825..7275
                     /locus_tag="ACOIAF_001155"
     CDS             4825..7275
                     /locus_tag="ACOIAF_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGZ2668175.1"
                     /translation="MFPIKYIDNNLVWNKDNEVFAYYELIPYNYSFLSAEQKFIVHDS
                     FRQLIAQSREGKIHALQIATESSIRSQQEQSKKLVTGKLKEVAYQKIDEQTEALVSMI
                     GDNQVDYRFFLGFKLMVTEEQLNLKNIKKSAWLTFTEFLHEVNHSLMNDFVSMPNDEI
                     NRYMKMEKLLENKISRRFKVRRLEINDFGYLMEHLYGRDGIAYEDYEYQLPKKKLQKE
                     TLIKYYDLIRPTRCVIEENQRYLRLEHEDKESYVSYFTVNAIVGELDFPSSEIFYFQQ
                     QQFTFPVDTSMNVEIVENRKALTTVRNKKKELKDLDNHAYQAGSETSSNVVDALDSVD
                     ELETDLDQSKESMYKLSYVVRVSADDLDELKRRCDEVKDFYDDLNVKLVRPAGDMLGL
                     HSEFLLASKRYINDYVQYVKSDFLAGLGFGATQQLGETTGIYMGYSVDTGRNVYLQPS
                     LASQGVKGTVTNALASAFVGSLGGGKSFCNNLLVYYAVLFGGQAVILDPKAERGNWKE
                     TLPEIAHEINIVNLTSDKDNAGLLDPFVIMKDKEDGATLAKEILTFLTGISTRDGDKF
                     PVLISAISKVSESEHRGLLNVITELRKENTTIANHIANHIASFTNYDFAHLLFSDGTV
                     ENAISLDNQLNIIQVADLVLPDKDTTFNEYTTIELLSVAMLIVISTFALDFIHSDRSI
                     FKIVDLDEAWAFLNVAQGETLSNKLVRAGRAMQAGVYFVTQSAYDVSKESLKNNIGLK
                     FAFRSTDINEIKQTLEFFGIDKDDENNQKRLRDLENGQCLLQDLYGRVGVVQIHPVFE
                     ELLHAFDTRPPVQRNEVE"
     gene            7275..9473
                     /locus_tag="ACOIAF_001156"
     CDS             7275..9473
                     /locus_tag="ACOIAF_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HGZ2668176.1"
                     /translation="MKERIKGAFTKKKIFHFLKMALFVVALSLILLSLLGTVAHATGL
                     VDDTINAGNLYSKYPLSNYQLDFFVDNSWSWLPWNWLDGIGKSVQYGLYCITNFVWTI
                     SLYLSNATGYVVQEAYKLDFINDMADSIGKSIQTLAGVTQNGFSSTGFYVGFLLLIIL
                     VVGIYVAYTGLIKRETSKALHAVINFVVVFVLSASFIAYAPDYIKKINEFSSDISTAS
                     LDLGTKIMLPNSDSEGKDSVDLIRDSLFSIQVEQPWLLLQFGNSNAEEIGTDRVEALV
                     SASPEDEDGKTREEVVKTEIEDNDNNNLTIPQVVNRLGMVFFLLFFNLGITIFVFLLT
                     GMMLFSQILFIIFAMFLPISFLLSMIPSYESMAKQAIVRVFNTIMTRAGITLIVTVAF
                     SISSMFYNISTDYPFFMVAFLQIVCFAGIYMKLGDLMSMFSLNAGDSQSMGRRIFRRP
                     YLFMRHRARRMEHRIARAVSAGGVAGAAAGSAVAGKRAERKNTASKENRSNTTSSMGQ
                     RAGSKVGAVLDTKNKVKDKANAVKENIKDMPTQTAYAVYSAKEKAKSSVSDFKRGMVQ
                     EQQSRQTGRLEKQEQRKKNIADKRMELQKAQEVRQAQRKADGSATTGATCPHERPATA
                     STIPKPSAEKMQEVKRPATALTSKASEPVKTNVIKERPLSSGASDRKATQSAQPVHRQ
                     NVEKVVSQETRQNDTKDRRIKVQQTQSVQKNQQTTEKSRNLVTKKGQKKK"
     gene            9470..10477
                     /locus_tag="ACOIAF_001157"
     CDS             9470..10477
                     /locus_tag="ACOIAF_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HGZ2668177.1"
                     /translation="MKLKHIALIGSLFPILFSLVLFFGVLISADSDDENSNFSSGITG
                     MNLSAEVLKHQPMVEKYARENGISEYVNVLLAIIQVESGGTAEDVMQSSESLGLPPNS
                     LDTESSIKQGCKYFASLLSSCKNQGMDDLNVAIQSYNYGGGYVGYVAGNGKKHTYNLA
                     ESFAREKSGGKKVTYTNPIAVAKNGGWRYQYGNQFYVELVNQYLTVPQVSGELTQKVM
                     NEALKYQGWKYVYGGSNPNTSFDCSGLTQWCYGKAGISLPRTAQAQYDATQHLPLSQA
                     KAGDLVFFHSTYNAGTYVTHVGILVSPTQMYHAGNPIGYADLSSSYWQQHLIGAGRIK
                     Q"
     gene            10494..11405
                     /locus_tag="ACOIAF_001158"
     CDS             10494..11405
                     /locus_tag="ACOIAF_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HGZ2668178.1"
                     /translation="MFKKNKKQTETIKEPKEKKVRTVKVGTHKKTVIALWVVLIASVS
                     FGVYKNFTAIDQHTTHEKEIIELRLQDTNGIKNFVKNFAKSYYTWNNSKEVIEARTQA
                     INGYLTKELQDLNVDTIRTDIPTSSTVTDVIVWNIEQSGTDTFSATYEVDQQIKEGEQ
                     TTAIKATYTVKVHVDADGDMVIVQNPTLAPAIEKSDYEPKTPEADRNLDADTVNDATS
                     FLETFFKLYPTATEKELAYYVSGNVLEPIGRDYFYSELVNPIFTKDGDNVKVKVAVKF
                     LDNQTKATQVSQYELVLHKDNNWKIVG"
     gene            11550..11783
                     /locus_tag="ACOIAF_001159"
     CDS             11550..11783
                     /locus_tag="ACOIAF_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HGZ2668179.1"
                     /translation="MKKGQHIFGTVKVGERGQIVIPKEAREIFDINAGDTLLVLGDEE
                     QGIAIVKADVMKELAVKILKGLGSLSGGFNDEK"
     gene            11773..12390
                     /locus_tag="ACOIAF_001160"
     CDS             11773..12390
                     /locus_tag="ACOIAF_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HGZ2668180.1"
                     /translation="MKNSISLFLPLALLLDLTIPFFLAPFYKGYSHSMQVMSVLGNNK
                     SPLHFIYNLWLVLCGIILLMSGFKVYEIVSQKSKNIAVILFIVILIYAIGGCILSGIF
                     SVGETKSLNTIPEKIHGYGAVLRFICLTFAPLLISLYWFRIEEIPLALFSMICFLLAF
                     IFFTIFVMADKPNYKDTIIAFEGLWQRLSLLFMYLPLGCLILLEK"
     gene            12410..12646
                     /locus_tag="ACOIAF_001161"
     CDS             12410..12646
                     /locus_tag="ACOIAF_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668181.1"
                     /translation="MIGLKKRDIKKALKAGAKAGGMSLADVRADIEATIDEAMNSTDP
                     EVQANFKKNFGNKRPAPEEYIYIITKKTVKGLKV"
     gene            12643..13557
                     /locus_tag="ACOIAF_001162"
     CDS             12643..13557
                     /locus_tag="ACOIAF_001162"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGZ2668182.1"
                     /translation="MKIERLIGIIFYLINRECVTTNELAQKFEVSRRTILRDIDTLTL
                     AGIPIYSELGVNGGYIINKDFKVDEKIIDSNNKEYILLALNSLRTVYGNKKIIETYEK
                     MKHLYNDVNINALPDVDFSVVTESPQIIRCVDLINKATKEQRTIKFIYTNSAGHTKKV
                     FLNPLHTYYRWYSWYVFGYDTSKNDYRMFKVVRMKNISLTKELFQTKDNIADLLSDFE
                     KRRNQTNITVTLSYKQEIDTLVNEYFKGKIVETVNDCFIREIEIKENDFMTFSILLGL
                     CDKVKILSPQKYKEKVLTHIRKIKKNFT"
     gene            13628..14242
                     /locus_tag="ACOIAF_001163"
     CDS             13628..14242
                     /locus_tag="ACOIAF_001163"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HGZ2668183.1"
                     /translation="MKKVYVYLLDDMAECEIGYILQAFSMEKLLKNGTKEFEVKTVGF
                     NKEPVLTLGGLTIVPDYSISEVDFNASTALLLPGSSTWGSKENQEILEKAQECLNKNI
                     LVGAICGATLALADYGILDKFKHTSNSLEYLNFFSRNYAGQSLYVCSNSVLDRNLVTA
                     NSAGSLEWTKNILNYLNVYSDNKITAFYNYYSTGNAKYYDELLK"
     gene            14276..14443
                     /locus_tag="ACOIAF_001164"
     CDS             14276..14443
                     /locus_tag="ACOIAF_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HGZ2668184.1"
                     /translation="MMPICNNKTRTKIRVDTELISFPLFCPKCKHETLINIKHGNVTT
                     IKEPDAKTQSR"
     gene            complement(14679..14930)
                     /locus_tag="ACOIAF_001165"
     CDS             complement(14679..14930)
                     /locus_tag="ACOIAF_001165"
                     /inference="COORDINATES: protein motif:HMM:NF024835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGZ2668185.1"
                     /translation="MKLFIGDIIQKLRKIKGLTQEQVANALGITTSSVSKWETNNTYP
                     DISLLSPLARLLGTAVDNVFNFKQKLSEEEIDTLLNRSK"
     gene            15198..15899
                     /locus_tag="ACOIAF_001166"
     CDS             15198..15899
                     /locus_tag="ACOIAF_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005359230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HGZ2668186.1"
                     /translation="MKAILKTNNLCKDFKKQKAVNNVSITVRENSIYGLLGPNGAGKS
                     TTLKMITGMLRPTSGKVLFNGHEWNRKDLEQIGALIETPPLYENLSAVENLEVRAKLL
                     NIPKTRIDKVLKMVDLQNTGRKKAGQFSMGMKQRLGIAIALLNSPKLLILDEPTNGLD
                     PIGIQELRSLIRSFPSKGITVILSSHILSEVQLIADDIGIISNGILGYEGQMNKDENL
                     ENLFVEVVRKSQEER"
     gene            15901..16644
                     /locus_tag="ACOIAF_001167"
     CDS             15901..16644
                     /locus_tag="ACOIAF_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015527239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HGZ2668187.1"
                     /translation="MKNYISSEYLKHKNTFAKKLVWLAPILTLCLNILAPMWYQQNSY
                     NWWYVFLYPGCLTLLSILIIQRDNGKLKFRAILSLPVDLKKVWYAKIITCIIYIVLAN
                     TILMLCNVAGGFIIQHIFDIPMTISPMQALFGTLCIILASIWNIPLCLWFTKKVGVFA
                     TLILNVGMSFMLGTLGANTSFWFICPYSWVTRLMVPTLGILPNGESVTGSALSTPLPL
                     VIMTICLSLLLLYTISKSTAKSFIKQEVL"
     gene            16644..17387
                     /locus_tag="ACOIAF_001168"
     CDS             16644..17387
                     /locus_tag="ACOIAF_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HGZ2668188.1"
                     /translation="MFSIIRCFQAEWLKSRHTPLLLVHVLFPLIGAGVFAGYFHISGW
                     SEMTNVTTFLEVLAIIFPFVIGIVVGMIVELENGAGHFQLILGTIRSRIAVYIGKLVY
                     LIVLATFATILCIFLFAVLYPVMPFSFYIKPLIMLILSVIPLYLISIPVGFSLGKSVS
                     MGIGIVGSLLSALLVTGLGDFIWKFLPWGWGVRFMDYCVLRYVNEGQFHSNLPEFQLG
                     FILMLVFTVVLFIISLVWFQRWEGVKENE"
     gene            17380..18189
                     /locus_tag="ACOIAF_001169"
     CDS             17380..18189
                     /locus_tag="ACOIAF_001169"
                     /inference="COORDINATES: protein motif:HMM:NF033433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NisI/SpaI family lantibiotic immunity
                     lipoprotein"
                     /protein_id="HGZ2668189.1"
                     /translation="MSKKMMILLFAVIICLSLAGCSLQDKIGDYSREQCYLNKENVTQ
                     FSYKGNDYTILADTVSNSGLGEWIGYIRQLAAVDESGKILLQENLKTATFQTLADLAD
                     LVDKAPNDAYIIPFLNVYAAPNADDYLIVDINGGYHKAVIDENIKGTDTVFDFKDIEQ
                     SMSGKFEINPQNATQLLCDGTVYQVTSDTVSNNELGSYIGILAENVIFNAETKIPLSK
                     EELRKIDWYGKNAGQHREQWIYKDIYEIHGTEKTEAVAVQINDRYYIAKRQ"
     gene            18252..18911
                     /locus_tag="ACOIAF_001170"
     CDS             18252..18911
                     /locus_tag="ACOIAF_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGZ2668190.1"
                     /translation="MATILMIDDERPILELVKNGLQKDGHLVTVYTSAAQVPLDKLNR
                     YDLIILDIMMPDIDGFSYCDKIRSLVDCPILFLTAKTMENDITFGLGLGADDYLTKPF
                     RITELRARVNAHLRREHRERHIALTFDRIKIDLSAKELRVDNIPVPLTKSEYLICEYL
                     ASNKGQVFSKEQIYEATFSLYGESDSSTISTHIKNIRSKFEKTGVQPIVTVWGIGYRW
                     E"
     gene            18977..20266
                     /locus_tag="ACOIAF_001171"
     CDS             18977..20266
                     /locus_tag="ACOIAF_001171"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HGZ2668191.1"
                     /translation="MSVVVPFACMMFASSIGLVTLADNNERQTKALVPILTATPNVSD
                     IHFPTGTKFLRLTKNYGYIDTNMSEEEKEKALMFAKTGFMDTSGKTQFIFITRDDEYI
                     VLQYVIGSQYLNAQFNQYLPSPEIMMISLITISSLGVCVYLTVHFAKELRKELTPILE
                     ATKEIENNNLDFTIGHSKILEFENVIISFDSMRNSLKGSLEKQWRSEKAQREQIASLA
                     HDLKTPLTVMQGNIDLLDETELDEEQKLYLSYAMSSSEQMKQYIKILIDISKASAGYQ
                     LQMEDVNFPEFWKHVLSQTEIICKDKGIVIQQTQHNFPQNITGDRMLLERALMNLVSN
                     SLEYSPENSTLYIDVDSKDNYLNICITDCGCGFSQEALDHAKERFYMADQSRSSKLHY
                     GMGLYIVDIIIKQHKGKLLLDNSTETKGAKVTIKLPL"
     gene            20627..21046
                     /locus_tag="ACOIAF_001172"
     CDS             20627..21046
                     /locus_tag="ACOIAF_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HGZ2668192.1"
                     /translation="MKPSDFQKTIQCQFECKLKKVTKGIVRNYQKELKRRKNKEIPFC
                     ELPELVIEKLSVLDDYDSDYTLFNVCGNDISILDDELAEALKKLSDRNRENLLMYYFL
                     EMSDTEIAKLQNISRSGVFQNRYNSLELMKKILKGEK"
     gene            21050..21298
                     /locus_tag="ACOIAF_001173"
     CDS             21050..21298
                     /locus_tag="ACOIAF_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGZ2668193.1"
                     /translation="MKHIFKKPSFYLISSATDGDEKAIEKILAIYDPYISKCCLRSLY
                     DEYGNVYIAVDMELKGRIREALIKMILDFDIPLETEEE"
     gene            21674..21877
                     /locus_tag="ACOIAF_001174"
     CDS             21674..21877
                     /locus_tag="ACOIAF_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HGZ2668194.1"
                     /translation="MNNTDVPIWEKYTLTIEEASKYFRIGEKKLRKLAEENIDAGWVI
                     VNGNRIQIKRKQFEKIIDTLDEI"
     gene            21956..23146
                     /locus_tag="ACOIAF_001175"
     CDS             21956..23146
                     /locus_tag="ACOIAF_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009271880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGZ2668195.1"
                     /translation="MSNVKRKDSKNRNLRNGESQRKDGRYVYKYTDIYGKPQFIYSWK
                     LVPTDKTPAGKRDDISLREKEAQIKKDLNDGIDTVGGKMTVCQLYEKKNSQRKNIKRA
                     TEKGRQYLMNALKNDLLGMRAIDTVKQSDAKEWAIRMSEKGYAYKTIDNYKRSLKASF
                     YMAIQDDCIRKNPFEFKLSDVLEDDTEQKVILTPEQEKHLLAFMETDKIYSKYYDEVV
                     LLLETGLRISEFCGLTTHIDMQNRILNIDHQLLKDSEMGYYIETPKTKNGKRELPLTE
                     RAYQAIQRILENRGKAQPLIVGGYSNFLFLNREGLPKVAGNYEGMVRGLIKKYNKYHT
                     DKLPNITPHSFRHTYCTNMANRGMNPNTLQYLMGHANITMTLGYYAHGTFQSAKAELE
                     RLAC"
     gene            23353..24531
                     /locus_tag="ACOIAF_001176"
     CDS             23353..24531
                     /locus_tag="ACOIAF_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HGZ2668196.1"
                     /translation="MTKNIWVQSVQSTIMKVLPMVFVGSLVTIVSVLKNYISFLPDLS
                     PINQYTFGLLGLFIAFLLPMEIMKNKKFESMSVVAGLAGAGLMLMMIRPEITNEGAIF
                     NFNRFGGEGMLVALVAGLFSGLIMSLFGSFSFFGEDSALPDFVSKWFDSMLPIAVIMF
                     IGWGLIYGLNFDMFEIIVRIFKPVSDIAQTLPGFVLLNFLVVFLYSLGISGWVLSPIT
                     YAIQLSAIAANAAAVASGGLATNIYTYEVVQTGWITLGGWGATLPLVLMMCLSRAGRI
                     KAIGRACIVPSIMNINEPVMFGAPVAWNPFLMLPMWIGTVVISITTYLSMSMGFVTIP
                     SKVFNLWYVPFGISTFMVNQDVRGLIVLAINIVLLFMIWYPFFKAYEKEELKLESQEA
                     "
     gene            24560..24913
                     /locus_tag="ACOIAF_001177"
     CDS             24560..24913
                     /locus_tag="ACOIAF_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HGZ2668197.1"
                     /translation="MTTEEHVIDEELVEVAMQIILRAGEARTEIKHALNDLERFDYKN
                     ADLKLAKAKEFMTEAHRAQTNIIQGEASGEKRAHSLLFAHAQDTLMTIFSELNITMSL
                     VGIVKSIEYKVNNSK"
     gene            24935..26380
                     /locus_tag="ACOIAF_001178"
                     /pseudo
     CDS             24935..26380
                     /locus_tag="ACOIAF_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427245.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            26479..27096
                     /locus_tag="ACOIAF_001179"
     CDS             26479..27096
                     /locus_tag="ACOIAF_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HGZ2668198.1"
                     /translation="MNVIKKFIFVILIINILGCGISLILQSSLGSDAITLLNEGIHIK
                     LGISYTIAGLIYNGTLLLIALLLNKKSLGLGSCVYVLVAGIFIDLYIYLLLDFNISSL
                     GIIIRIMSFIIGHILMCSGFAMLIKFDIGMSPLDAILIYIEGKFKYPYKVLKTIVDVI
                     FLILGVYLGGNLGFGTIFSILCTGTTISIVSKMISVNKKRYYSNI"
     gene            27392..28144
                     /locus_tag="ACOIAF_001180"
     CDS             27392..28144
                     /locus_tag="ACOIAF_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HGZ2668199.1"
                     /translation="MIIELNKSNSENLTKTELQIIKFINENESILPELSIVEIAHETY
                     SSPATVSRAIRKCGLNGFNELRYRLTVKDENDDIQNMGDIINKSFIEAQRVIEQISLT
                     TLINIIKTINKSSKIYILARGLTEYVAKEFSLKLQLLDFNVVFINDPNIMKIKSKSIK
                     KDELIIIFSLNGNTIELVESAQNANLVGAKVITCCCSESAKLIKYSDLYIVGYKHEHI
                     SISEYEVSSRLPLYIISRIIIDYMVRYNNSEN"
     gene            28246..29166
                     /locus_tag="ACOIAF_001181"
     CDS             28246..29166
                     /locus_tag="ACOIAF_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HGZ2668200.1"
                     /translation="MIYTLTTNPAIDMNISTNGIKRKLVNRTSNAVYSPNGKGLNVTF
                     VLGHYGIESKILGFFGGFSGKYIVEESEKRGFDVLPTWVEDTTRINIFLNDGSDEFKF
                     VNSGSYVNEKQKLDMLDKIQSLADISYLSISGSLPPGIDDNYYEDIFKICKNKSIKTI
                     LDISSPKLKELLQYNPYLIKPNDEEIKDIFGIIVRDEEDIKDVLKLLHLKGAQNILLT
                     LGEKGSYFYNGKSIYYASAQPVTVVSSACAGDSALAAFLSIWLENPEHIEEALKRSAA
                     TGASVAESNGIGNLENVEDYIKNIRVRKVV"
     gene            29169..31085
                     /locus_tag="ACOIAF_001182"
     CDS             29169..31085
                     /locus_tag="ACOIAF_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HGZ2668201.1"
                     /translation="MGKKMILGVTGCPTGIAHTFMAEEALKNSARELGCDIKVETNGA
                     IGVENKLTAKDIEMADAIIVACDKNVDMDRFNGKPVIEVPVKEGIHKANELIQRCIDG
                     KVAIRNGNLSSSKIAEEGNLSFGQKLYKDLMNGVSHMLPLVVAGGVLTAISFLWGIYS
                     FDPNSEQYNQIAATLKSVGGYSMNLMVPVLAAFIAQSISGRAGMLAGLVGGMISFDTG
                     SGFLGGIISGFLAGYLVKLFVHLLRKLPRQLEGLKSIFIIPIVSVGLVGVAMLLLGGP
                     CSALNNAMMNFLSGLQNSSPIILGIVIGCMSAFDMGGPVNKAAYVTGTMLLGQGNYLF
                     MAGVSAACITPPLVIAIASTLFKNRFTEEDRAAGLINYILGSTHITEGAIPFAAKNPL
                     KVLPVLMIASSISSILTYIMKIEVPAPHGGFLVLGLVNKPLLWVGCIFVGSLVGAVLY
                     MMVTPKVVNEKNTIENTNATKDEHKNESAESENVSNNISLYSEETVILDVKGKNKTDV
                     IDEMIGILDKSGVLLDKKKFKEEILKREEISSTGFGMGIAIPHAKTDAVKVPRVAVGV
                     SKEGFDFESEDGSLAHIIFMIAATDNGDNLHLKTLSQLSSKLMNEEFLNELLNSKTSR
                     EIVSKLNNEEIKSV"
     gene            31105..31974
                     /gene="gatY"
                     /locus_tag="ACOIAF_001183"
     CDS             31105..31974
                     /gene="gatY"
                     /locus_tag="ACOIAF_001183"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HGZ2668202.1"
                     /translation="MALVTTKQILLDAQEGHYAVGAFNVENMEMVMAVIEAAEELKSP
                     VILQTTPSTIKYAGLDYYLANVKVASEKASVPVAMHLDHGSSFGLAMQALRVGYTSIM
                     IDGSHESYEDNISISKSVVDACMPSDIPVEAELGKVGGKEDDLDGGDGGAYTDPLEAK
                     EFVERTGVSSLAVAIGTAHGLYKGEPKLDLDRLSEIREVVSVPLVLHGGSGIPDEVIK
                     ESIKRGICKVNYATELRIAYSNGVKDVLNSDPEVIDPKKYGKKGLESVKDFVKSRMEV
                     CGCVGKAIEIVKC"
     gene            32484..33947
                     /locus_tag="ACOIAF_001184"
     CDS             32484..33947
                     /locus_tag="ACOIAF_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HGZ2668203.1"
                     /translation="MITGELRSKVDKIWETFWTGGLTNPLEVIEQFTYLLFIKGLDEV
                     ETKNEAEATLLGVSFERIFDDEHQHLRWSKFSNEGNSEKMYEIVQNEVFPFIKKLHGN
                     KDSAYAKYMGDAIFKIPTPLMLSKIVDGISNLELSKDRDTKGDLYEYLLSKVATAGTN
                     GQFRTPRHIIDMIVRLIKPTPEDIIVDPAAGSAGFLVSSQQYLRDNHSSLFLVQGLKE
                     HFNNGMFYGFDMDRTMLRIGAMNMMLHGVDNPNIEYKDSLSEVNTDKDKFTLVLANPP
                     FKGSLDYEAVSADLLKVTKTKKTELLFLALFLRVLKIGGRCACIVPDGVLFGSTGGHK
                     SIRKEIVDNHKLEAIISMPSGVFKPYAGVSTAIMIFTKTGTGGTDKVWFYDMKADGYS
                     LDDKRNEIKDSDIEDIIKRFDNLDGEVDRKRTEQSFFVDVEEIRENGYDLSINKYKEV
                     VYEEVVYDAPSVIIGRVRELEKEIVDGLEELERMLEV"
     gene            33951..35123
                     /locus_tag="ACOIAF_001185"
     CDS             33951..35123
                     /locus_tag="ACOIAF_001185"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HGZ2668204.1"
                     /translation="MEYRLNEVFEFIRNGASIKQFDTKFGYPITRIETIANSYIDTNK
                     MGYADIKELGKLEDYLLKNGDILMSHINSEKHLGKSAIYEDINKKIIHGMNLLCLRPD
                     KEVCYPKYLNYFFNSNEFRRQLPRIIKKSVNQASFSVNDLKNLKVDLPEMEAQKDISK
                     VLDKAQELIDKRKEQIEALDELVKSRFIEMFGNFKEQKFVLLKDCTNFIDYRGKTPIL
                     SENGIRIINAKSVGNGVFKYINEYISENTYNSWMKRGFPVAGDVLFVTEGHTFGNVCR
                     IPKDLKKFAMGQRIITIQGDKKILNNAFLAQYMQTKAFRVEIDKYKTGSSAQGIRSKE
                     LQKILIPVPSIELQNKFEEFVNQVDKLKVGIKTSLKELEDNFNSLMQKAFKGELFN"
     gene            35156..35464
                     /locus_tag="ACOIAF_001186"
     CDS             35156..35464
                     /locus_tag="ACOIAF_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668205.1"
                     /translation="MNQIELDLYQNMVNIYKEADLQCNYKPTRFLQLINNKGALLAAK
                     ELINKEGATEGFTRLWECKRLDLSLEVLVLKEKYKELFTDEEREICINRLKDYGYKFN
                     "
     gene            35497..35877
                     /locus_tag="ACOIAF_001187"
     CDS             35497..35877
                     /locus_tag="ACOIAF_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HGZ2668206.1"
                     /translation="MRNIPNEIKNSIINVFPKENTLAIIIFGSYGTDKHTSESDIDIA
                     WIPIKKVPITELATKTEALRNILDIDVDLKIVTDNYTIALKKAILEGEVIYESKDFKD
                     YINKFYFENSDVIDILDWRDMYGN"
     gene            35867..36295
                     /locus_tag="ACOIAF_001188"
     CDS             35867..36295
                     /locus_tag="ACOIAF_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668207.1"
                     /translation="MVIRNKLSWNSIEEVNRDFGSCLYDLQNFKILYDKEEMPELWAR
                     YIKEGFKSYMVSFLELTKAMLYYKSIDLNIKSKNFYDHLLTCEYHNLLPKNSSIVIET
                     LRKLRNDDSHGYDIPQFEDMYELFTENEEVFVSIRNSCKK"
     gene            36345..39680
                     /locus_tag="ACOIAF_001189"
     CDS             36345..39680
                     /locus_tag="ACOIAF_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HGZ2668208.1"
                     /translation="MGNFDFLQKKKNFNSFNTACLEAEKSILVSPSTCATITRRALEL
                     AVKWLYANDSDLVLPYQDNLSTLIHNNSFIELIDYDMLPLLKYIVKLGNLSVHTSANI
                     ERGEAILSLNNLHQFVSWIDYCYSDEYTAEDFNEDLLLHGEEKRTRPDELKNLYERLS
                     SKDKKLEEVIKENEELRKSLTEKRKVNTVNYDFKIDEVSEFETRKRYINIELKLAGWE
                     FGKDIGEEIEVQGMPNESGVGYVDYVLYGENGKPLAVVEAKRTSKDPKIGQQQAKLYA
                     DCLEKRYSQRPVIFLTNGFDMYIWDDYSDRKVYGFYKKSELQLMIDRRKSKKSLSSVT
                     INDEISNRYYQKEAIRAVCEALENKQRKTLLVCATGTGKTRIAISIVDVLSRHNWIKN
                     ILFLADRKALVKQAKKSFTKLLPNLALCNLLDSKDSPEDARMIFSTYPTMMNAIDDTK
                     SKDGKRLFTPGHFDLIIIDESHRSIYKKYKSIFDYFDSYLIGLTATPKDEIDKNTYSV
                     FDMENGVPTYAYEYNKAVEDGYLVDYTSIEFKTKIMEDGIKYDELSDEEKEEYENTFN
                     DDESIGDEIGNNAVNEWLFNSDTIDLVLNKLMTEGLKIEGEEKIGKTIIFAKNTKHAR
                     AIVERFNKLYPKYGGNFVKAVDYSINYVDSIIDDFSDKNKLPQIAVSVDMLDTGIDIP
                     EILNLVFFKKVRSKTKFWQMIGRGTRLCPDLLGVDMDKERFLIFDFCNNFEFFKFNPK
                     GFEGNKAETLTEKLFNIKVSMVKELQDIKYIEGEYAELRKELLEELITSVKALNEDSY
                     IVRMNLSYVHKYKNENVWSNIGAVAQNEIREYISPLITSYSDDELAKRFDVVMYNIQL
                     AYLQNNNASKGIRHVMATAEKLSKLGTIPQIQQQKYTIEKAMTEDFWEDSDIFEVEEV
                     RISLRELIKYLEKSSQKIYYTSFEDMIVAEDRNDSVYNANNLKNYKKKVEYYLNSHKD
                     ELAIFKLRNNKKITKQDVETLEEILLKQLGNCDDYKKEFGDTPVSQLVRKLVGLDREA
                     ANEAFSEFLNNKSFNTKQIHFVKLIVDYVVKNGFIEDNKVLMEDPFRTVGSIIDLFEN
                     HIEERNKLIKTINKIKENASEIG"
     gene            40061..42124
                     /locus_tag="ACOIAF_001190"
     CDS             40061..42124
                     /locus_tag="ACOIAF_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HGZ2668209.1"
                     /translation="MKAGEIEFLSYLEGSNKSFVVPVYQRNYNWKKEQCKRLFDDLED
                     IIDSGFRTHFLGSVVSIYSMGKEYLIIDGQQRVTTVSILLIAMCQIIKEFGESKGIIT
                     TEEQIKECYLINKHSRDKDGKIKLKPIAEDRKAYYELFEEEMNTDRNSNIVSNYLYFY
                     NRIKEDNISIDDLFDAIQKLTIVEIELKNGEDDPQLIFESLNSTGLDLSEADRVRNFV
                     LMGRSSKLQEEFYNNYWYKIEKNTSSNVSSFIRDYLTIKERSIPNKNKVYFSFKRYVS
                     DNGIDIEILLKDLLKFSNYYREISYSCTDDNDVNKVLKRINKLEIFVSYPFILELFDD
                     YNEGIINKKVLVESLNLVECFILRRIVCDVPPNALNKVFMNLGREIKKFSDYKENYSN
                     ILRYIFINKKSSQRFPTDSEFSNAFETKDIYNTKSKNKLYILERLENYNNKEIVDLEN
                     LINNNMLNIEHIMPQTLTNKWKESLGDNYKEIHDKYLHTIGNLTLTGYNSKLSNKTFE
                     EKKEMEDGFKDSRLYLNKYISSIDKWNEEEIKNRTKILLDRAVEIWKYPSVTYEPPSN
                     SENIFTLEDDDVNFTNESIVSFSILGESYKAKNWTEFYEQVALTLYDLDSVRFNSIVD
                     KTYKRDFIDNIKSRDAYKLRKGIKISENIYLETNLNTEAKLNIIRFLLNEFDIELNEV
                     IFSIK"
BASE COUNT        15242 a         5850 c         8513 g        12625 t
ORIGIN      
        1 aaatgtgtat cacaccaaag aagaaatgat tgattgcgtc aatgcctttt atgagggcat
       61 ggtacagcga agtgaggaaa tgaagcgaca cccgaactat aagacgggcg aaaactatgc
      121 ctatctggga ctgccaccct gctttcttat ctttgatgaa tatgtggcat tttttgaaat
      181 gctggggacg aaagaaagcg tgggcttact tagccagtta aagaaaatcg ttatgttagg
      241 acgacaagca ggttatttcc ttatcgttgc ctgccagcgt ccagacgcaa agtatttctc
      301 ggacggtatc agagataact tcaatttccg tgtgggactt gggcgtatca gcgaattagg
      361 ttacggtatg ctttttggtt cagatgtgaa aaagcagttt ttccagaaac gtatcaaggg
      421 gcgtggctat tgtgatgtgg gaacaagcgt gataagcgaa ttttacacgc ctttagtccc
      481 gaaaggacat gattttttgc agactatcgg ctctcttgct caagcaaggc aggacgggac
      541 ggcgacgtgc gaagcgaaag gcgacggcac ggactagccg ttgctggtgt ggcgaagcca
      601 cgccagcagg taaaacccct cggtatctaa cagaggggta cgtttgcaaa tagacaatag
      661 acgaaaaaca caggaaacat aaggcttatc gcatggtttc ctagcaaaaa tcggctgtga
      721 agtcgcctta aaaaacttca cacttgacag attgggggta tggttctgaa tgaagaacaa
      781 tggataaaag aattacggga aaaacggatt gcttacggta tctcacaagg cagactggcg
      841 gtggcttctg atatcacaag ggaatatctc aacaagatag aaagcggaaa aatgaagccg
      901 tcaaaggaac ttctggaaac tctgcataaa gaactggcaa ggttcaatcc agaagcaccg
      961 cttaccatgc tgtttgatta tgtaaaaatt cgttttccca cgctggatat acagcacatc
     1021 atcaaagata tattaaaact gaatatcaat tatatgctcc atgaagatta cggacattac
     1081 agctatacgg agcattattc tcttggggac atctttatct atacgtcggc tgacgaagaa
     1141 aaaggtgtcc ttttagagtt aaaagggcgt ggttgccgac agtttgaaag ttacctgctg
     1201 gcacagcaaa gaagctggta tgactttctc atggacgcac tcgtagacgg tggcgtgatg
     1261 aagcgtatcg accttgctat caacgaccat acgggcattt tggatatacc agagcttgcg
     1321 gaaaaatgca ggaaacggga atatatcgga aagtccagaa gctataagtt ttatcagtcg
     1381 ggcgagctta tcaagcatag agaggacgac agagaatata tgggacgcac cctttatctt
     1441 ggttcgctga aatcagatgt gtatttctgt atctatgaaa aagactatga gcagtatgtc
     1501 aagttaggga cacctttgga aaaagccgaa attatcaacc gttttgagat acggcttcgc
     1561 aatgaacgtg cttactatgc agtacgggat ttgctgacct attatgacgc agagcagacc
     1621 gccttttcta tcatcaacca gtatgtgcgg tttgttgatg aagaacccga caagcgaaaa
     1681 aatgactgga aactcaacga gcgctgggct tggtttatcg gggataatcg gcaaagtctg
     1741 aaactgacga caaagccaga gccttatacc ttagaccgca cgcttcgttg ggtacaaagg
     1801 caggtagcac cgaccttgaa aatgctgaaa aggattgata agggaaacgg aacagactac
     1861 atgaaaacta tcgaacagca ggcaaagctc acagaaaagc atgaaatgat aatcaaacag
     1921 cagacgaccc ctgcaaaaga tttagtagaa agttaggagt gatgaaaaaa tgtgggaatt
     1981 attgaaagac ttcctgctgg tatctgttgg aacgggtatc ggcgtagcct tgatgtgtat
     2041 tttaagtgtt ggcaaagaag ctgactatga aatgaagaaa ttaaaagaaa gcgaggacaa
     2101 ataacatgaa tttcgggcaa aatttgtatc aatggttttt atcaaacgca cagagcttag
     2161 tgcttatggc gattgtggtt atcggtatct acttagggtt caagcgtgag ttttcaaagc
     2221 tcatcggctt cttggtagtt gccttgattg ctgtcggttt ggtgttcaat gctggcggtg
     2281 tgaaagatgt actgttagaa ctgttcaaca agattatcgg tgcataaatt tttattgtgg
     2341 tgctaatatg cgaatgttat aatatggata tataaaattt tgatttatgg gggtattctt
     2401 ttgaagaaag caatgatata tgttcatggt aaaggtggaa gttatcttga agccgaacag
     2461 ttcaaaaata tttgtttaga ttttgatgtt tttggagtag attataatga ctatttcccg
     2521 tggattgtaa aaaatgaaat tatggaagtc tacgataaga tatctgaaaa atatgactat
     2581 atatatgtac ttgctaatag tatcggagct tattttacaa tgcacacatt acagaatttc
     2641 aagattgaaa aagcattatt catatcgcct atacttgata tggaacaact tatagtagat
     2701 atgatgagct gggcgaatgt gtctgaaaag gaactttctg aaagaaaaga aataccaact
     2761 gattttggcg aaacattatc gtgggaatat ttatgttttg ttcgggaaaa cccgataaat
     2821 tggaaaacgc ctaccgaaat tttgtatgct gaacatgata atcttacatc acgtcaaacg
     2881 gtagataaat ttattgatag acattgtgca aatcttaccg ttatgaatga cggagaacat
     2941 tggtttcata ctaacgaaca gattttcttt agagataatt ggctaagaaa agttatacaa
     3001 tagaatttag attttatatc catacacaca aagcagttag tcttaggatt gactgctttt
     3061 ttcttaccta aaatcagatt ggagtgatga aatgagcgat atttttaagg atatgcaggc
     3121 aaacgtcggc tgtgattata tttctaacct gccctcttac aagcgtaaag tgtggcatga
     3181 aatgaagcga ttaccgctta cgaattatga tataaaacag ttagaagatt tttcaaagta
     3241 tgtgtttggt atgtcgtacc agagcttaaa agatgtgatg aaacaacaga aaggacgtga
     3301 ggaacaatgc aggaaacaag ggtgctggtg gaaacgagag gaacaactgg caaagaaaca
     3361 acatcatact ggttcgacct accgatagat gttgccgagt ttgaagaaaa gttaggtgtc
     3421 ggtgcagaaa gtcaagatta ccgcattatc gaaaaggtgt tgccttatgc tgatgaagtc
     3481 cacgaacaaa caagcgtgta ccagctgaat gaattagatt ttatgtaccg ccagctacca
     3541 gccgatatgc aggaagaata tatagcactt cttaccgtgt ttgaaaactt agaagcactt
     3601 tatatttgca ggaatgtaat tacagtttat ccagactgta aaagcatgat agatgttgca
     3661 aaacaaaagc tgatgaatga cccgacattc aaacatctgt ccgaggactg tcaagcatac
     3721 tattttgact ttgaagccta cgcttctcac ttgctggaaa atagacgcta tcttgtaacg
     3781 gaacatagtg tctttgaact gccagcgtag gaaatgaggt ggtgcttatg attgatgata
     3841 tggcagttta tattgctaat cttggcaaat ataacgaggt ctatttagtg ggtgcttggt
     3901 ttacatttcc cattgacgag gaagatgtga aagaaaaaat cggcttgaat gaacagtatg
     3961 aggaatacgc aatccatgat accgacaact tccccattgc gattggcgag tatgtttcca
     4021 ttgatgaact caatgagatg tatgaaatgc tagaggaact tcccgactat attgtagagt
     4081 gtctggatga atttatcggt cattacggga cgctggaaga agtcgtggaa cacaaggacg
     4141 atatttacta ttatcccgac tgtgaaacca tgacagacgt tgcctattat tacatagacg
     4201 aattgcaggc actaggcgac attccaccca gcttgcagaa ctacattgac tatgaagcct
     4261 acgggcgaga tttggatatt ggcggttgct tcattgaaac aagccgaggt atgtgcgaga
     4321 taccatatta acgctggaag atcagcgaca agcattgttg ctactgacag cgtatttctc
     4381 tttaaaggac agtctgaaaa tggctgtcct tttctctttg aagaacttac gaaaggagct
     4441 gaaagaactt gaaaaagatt aaatcttaca cgggtatctg gaacgtggaa aaagtcttat
     4501 atgcaatcaa tgattttaac ttgccctttc ccgttacttt tacacagatt acatggtttg
     4561 tgattacgga atttatcatc attctgtttg gggatattcc cccactttcc atgattgagg
     4621 gagcatttct caaatacttc ggtattcccg ttgctctcac ttggtttatg tcgcagaaaa
     4681 cctttgacgg aaagaagccg tacagctttt tgaaatcaca gataacctat gccctgcgac
     4741 caaaaatcac ttatgcagga aaagccgtaa aactgcataa gcagatactc aatgaaacaa
     4801 tcacggcagt aaggagtgtg aactatgttc ccgataaaat atattgacaa taaccttgtc
     4861 tggaacaagg acaatgaggt attcgcctac tatgagctga taccgtacaa ctattctttc
     4921 ttatccgcag agcagaaatt tatcgtgcat gacagctttc gacagcttat cgcacagtcc
     4981 cgtgagggta aaattcatgc cttgcagatt gccacagaaa gctcaatccg aagtcagcag
     5041 gaacagtcaa agaaactggt aactggaaaa ttaaaggaag ttgcttacca gaaaatagac
     5101 gaacagaccg aagcgttagt atctatgatt ggggacaatc aagtggacta ccgctttttt
     5161 cttggcttca agctcatggt tacggaagaa cagctcaatc tgaagaacat caaaaaatcg
     5221 gcttggctga cgttcacaga atttctccat gaagtgaacc actcgctgat gaatgatttt
     5281 gtttccatgc cgaatgatga aatcaaccgt tacatgaaaa tggaaaagtt actggaaaat
     5341 aaaatctccc gtcgctttaa ggtgcgtcgc ttggaaatca atgattttgg gtatctcatg
     5401 gaacatcttt acggcaggga cggtatcgcc tatgaagatt atgagtacca gctaccaaag
     5461 aagaaattac agaaagaaac gctgataaag tattacgacc ttatccgtcc gacaaggtgt
     5521 gtgattgaag aaaaccagcg atatttacgc ttggaacatg aggacaagga aagctatgtg
     5581 tcctacttta ctgtcaatgc gattgtaggc gagcttgatt ttccgtcgtc tgaaatcttc
     5641 tatttccagc aacagcaatt tacttttccc gttgatactt ctatgaatgt agaaatcgtg
     5701 gagaaccgaa aagcattaac gacagtaaga aacaagaaaa aggaattaaa agaccttgac
     5761 aatcacgcct atcaagcagg aagtgaaacc agctcaaatg tggtggacgc attagacagc
     5821 gtggacgagc tggaaacgga cttagaccag agcaaagaaa gtatgtataa gctctcctat
     5881 gtagtgcgtg tatcggctga tgatttggac gaattaaaac gccgttgtga tgaagtcaag
     5941 gacttttacg acgacctcaa tgtcaagctg gtgcgtcctg ctggggatat gctggggctt
     6001 cattctgaat ttcttcttgc cagcaagcga tatatcaatg attatgtgca gtatgtaaaa
     6061 tcagattttt tagctgggct tggctttggt gcgactcagc agttagggga aactacgggt
     6121 atctatatgg gctattccgt tgatacggga agaaatgtgt atctgcaacc gtctttagct
     6181 tcgcagggcg taaaaggcac agttaccaac gcccttgctt ctgcttttgt cggaagtctt
     6241 ggcggtggaa agtcgttctg caacaatctt ctggtgtatt atgcggtgct gtttggcggt
     6301 caagcagtca ttttagaccc aaaagccgag cgtggtaact ggaaagaaac gctcccagag
     6361 atagcccatg aaatcaatat cgtaaacctt accagcgaca aagataatgc aggacttctt
     6421 gacccgtttg tgattatgaa agataaagag gacggagcaa cgctggcaaa agaaatctta
     6481 acctttttaa cgggtatttc tacccgtgac ggagataaat tccctgtgct tattagtgca
     6541 atcagcaagg tatctgaaag cgaacacaga ggactgttaa atgttatcac agaattacgc
     6601 aaagagaata cgacgattgc aaaccatatc gcaaaccata ttgccagttt tacaaattat
     6661 gattttgcac acctgctgtt ttcggacggt acggtagaaa atgctatcag tctggataac
     6721 cagctcaaca tcattcaagt agccgacctt gtgttgccag ataaagatac cacttttaac
     6781 gaatacacga ccattgaatt attatcggtg gcaatgctga ttgtgattag tacctttgcc
     6841 cttgatttta tccattcaga cagaagcatt tttaagattg tggatttaga cgaagcatgg
     6901 gcgttcttaa atgtggcaca aggcgaaacc ttatcaaata agctggttcg tgctggacga
     6961 gctatgcagg caggcgttta ttttgttacc cagtctgcct atgatgtttc aaaagaaagt
     7021 ctgaaaaaca atatcggctt gaagttcgca tttagaagca cggatataaa cgagataaaa
     7081 cagaccttag aatttttcgg tatcgacaag gacgacgaaa acaaccagaa acggcttcgt
     7141 gatttggaga acggacaatg cttattgcag gacttatacg ggcgtgtcgg tgtagtacag
     7201 atacaccccg tctttgaaga actgctacac gcctttgata ccagaccgcc cgtacagaga
     7261 aatgaggtgg agtgatgaaa gaaaggataa aaggtgcgtt cacaaaaaag aagattttcc
     7321 actttctcaa aatggctctg tttgtcgtag cactctccct tatcctgctt tcacttctgg
     7381 ggacggtggc tcatgcaacg gggcttgtgg acgataccat aaatgcagga aatctttatt
     7441 caaaatatcc cctttccaac taccagcttg attttttcgt cgataatagc tggtcgtggc
     7501 ttccgtggaa ctggctcgac ggtatcggaa aatcggtaca atacgggctg tactgcatta
     7561 ccaactttgt ctggactatc agtttgtatt tgagtaatgc aacgggctat gtggtgcagg
     7621 aagcctataa gctggacttc atcaacgata tggcagacag tatcgggaaa agcatacaga
     7681 cccttgcagg cgtaacacag aacggctttt cttctacggg cttctatgtt ggtttcctgc
     7741 ttctcattat cttagtagta ggaatatatg tagcctatac gggactgata aaaagagaaa
     7801 ccagtaaggc acttcacgcc gttatcaact ttgtggtggt gtttgtgctg tctgcttcgt
     7861 ttattgccta tgctcccgac tacatcaaga agataaatga attttcatct gacatcagta
     7921 ccgcttcttt ggacttggga acaaaaatca tgctccccaa ctctgacagc gagggtaagg
     7981 acagcgtaga cttgatacgg gacagcctat tttctattca agtggaacag ccgtggttac
     8041 tcttacaatt cggaaacagc aacgcagagg aaatcgggac agaccgtgtg gaagctctcg
     8101 tatcggcaag cccagaggac gaggacggca agaccagaga ggaagtcgtg aaaacagaaa
     8161 tcgaggacaa cgacaacaac aatctgacga taccgcaggt cgtgaaccgt ttaggcatgg
     8221 tgttcttcct attgtttttt aacctaggga taacgatatt tgtattcttg cttacgggca
     8281 tgatgttatt tagccagata ctttttatta tcttcgctat gtttctgcct atcagttttc
     8341 tactctccat gataccgagc tatgaaagca tggcaaagca ggcaatcgtg agggtattta
     8401 ataccataat gacacgggca ggaattacgc tcattgtaac ggtggcgttc agcatttcca
     8461 gtatgtttta taacatttcc acagattacc cgtttttcat ggtagcgttc ttgcagatag
     8521 tgtgtttcgc tggtatctat atgaagctgg gcgatttaat gagtatgttc tctcttaatg
     8581 ctggcgacag tcaaagcatg ggacgcagga tattccgcag accatatctg tttatgcgac
     8641 atcgggctag acgtatggaa caccgtattg caagggctgt aagtgctggc ggtgttgctg
     8701 gtgcggcggc tggaagtgcc gttgctggca aaagagccga aagaaaaaat acggcttcta
     8761 aagaaaatcg gagcaatact acttccagta tgggacagcg tgcaggctca aaggtgggtg
     8821 ctgtcttaga cacgaaaaat aaagtgaaag acaaggcaaa cgctgtcaaa gaaaatatca
     8881 aggatatgcc gacacaaaca gcttatgcgg tatattccgc aaaggaaaag gcaaagtcca
     8941 gcgtatcaga cttcaagcgt ggcatggtgc aggaacagca gtccagacag acgggacgtt
     9001 tggaaaagca ggaacagcgt aagaaaaata ttgctgacaa gcgtatggag cttcaaaagg
     9061 cacaagaagt aaggcaggca cagcgaaagg ctgacggatc agcgacaacg ggagctacct
     9121 gtccccatga gagaccagcc acagcttcaa ccattccaaa accgagtgct gaaaaaatgc
     9181 aggaagtcaa acgccctgcc acagctctga cttctaaagc aagtgagcca gtcaagacaa
     9241 atgttatcaa agagcgtccg ctatcttcgg gtgcttctga tagaaaagcg acccagtcgg
     9301 cacagccagt acataggcag aatgtagaaa aagtggtatc gcaggaaaca cgccagaatg
     9361 acaccaaaga ccgcagaata aaggttcagc agacgcagag cgtccagaag aaccagcaga
     9421 ccacagagaa aagccgtaac cttgtaacaa agaaaggaca gaagaaaaaa tgaaattgaa
     9481 acatatcgct cttattggaa gtctgtttcc tattctcttt tctctggtgc ttttctttgg
     9541 tgtcttgatt agtgcggaca gcgacgacga gaacagcaac ttttcttctg gcattacggg
     9601 tatgaaccta tccgcagaag tcttaaaaca tcagcctatg gtagaaaaat acgccagaga
     9661 aaacggtatc tccgagtatg tcaatgtgtt gctggctatc attcaagtag aaagtggcgg
     9721 tacggcagaa gatgttatgc agagttcgga aagtcttggt ttaccgccca actctttaga
     9781 tacggaaagc tcaatcaagc aggggtgtaa gtattttgcg tccctgcttt cttcctgcaa
     9841 aaatcaaggt atggacgact tgaatgtagc gatacagtcc tataactatg gcggtggcta
     9901 tgtgggatat gtggcaggaa acggaaagaa acatacctac aatcttgcag agagcttcgc
     9961 ccgtgagaaa tcgggtggaa agaaagtaac ttacaccaac ccgatagccg ttgcgaaaaa
    10021 tggtggctgg cgttatcagt atggcaatca attttatgtg gaattagtca atcagtattt
    10081 aactgttccg caggtatcgg gagagctgac acaaaaggtc atgaatgaag cgttgaaata
    10141 tcaaggctgg aagtatgtgt atggcggtag caatcccaac acttcctttg actgttcggg
    10201 acttacgcaa tggtgctatg gaaaagctgg tatctcctta ccgagaacgg cacaagccca
    10261 gtatgacgca acccaacatc tgccactctc gcaggcaaag gctggcgatt tggtattttt
    10321 ccattctacc tataacgctg gtacttatgt aacgcacgtt ggtattcttg tttcgccgac
    10381 gcagatgtac catgcaggaa atccgatagg atatgcagac ctaagtagta gttactggca
    10441 acagcactta atcggtgcag gacgaataaa acaatagaaa ggacttgaaa aatatgttta
    10501 agaagaataa gaaacagaca gaaactatca aagaaccaaa agaaaaaaag gtgcgtactg
    10561 tcaaggtagg cacacataag aaaaccgtga ttgcgttgtg ggtggtgctt atcgcaagcg
    10621 tgagcttcgg ggtgtataag aattttacgg ctatcgacca gcacaccacc catgaaaaag
    10681 aaatcattga gcttcgcttg caggacacca acgggataaa aaatttcgtg aagaattttg
    10741 cgaagtccta ttacacatgg aataacagca aagaagtgat tgaagcaaga acgcaggcaa
    10801 tcaacggtta tctgacaaag gaattgcagg acttgaatgt ggataccatt agaaccgata
    10861 taccgaccag ttccacggtt acagatgtga ttgtgtggaa tatcgaacag tcgggaacgg
    10921 acactttttc tgccacctac gaagtagatc agcagataaa agagggagaa cagacaacag
    10981 caattaaagc aacctatacc gtgaaagtcc atgtggacgc tgacggggat atggtaatcg
    11041 ttcagaaccc tacccttgca ccagcaatcg aaaaatcaga ctatgagcct aaaacaccag
    11101 aagcagacag aaatctggac gctgatactg tcaatgacgc tacctcattt ttggaaacat
    11161 tctttaagct atacccgaca gctacagaaa aagaacttgc ttactatgta tcgggaaatg
    11221 tgcttgaacc tatcggcagg gactactttt attctgaatt ggtaaaccct atctttacaa
    11281 aggacgggga caatgtaaaa gtcaaggttg ccgtgaagtt ccttgataat cagacaaaag
    11341 cgacgcaggt atcgcagtat gaacttgtgc tacataagga taataactgg aagattgtag
    11401 gataagcaaa tatggcgtgc atttctataa gagatttgca cgctatccat gttataagga
    11461 attgacaatt cttatttttc gcattatact atttatgtaa atatgaaaaa tatgattcgt
    11521 aaataaatat gataagaagg agaaaaatga tgaaaaaagg acaacatatt tttggaacgg
    11581 taaaagttgg agaacgggga caaatagtta tcccaaaaga agcccgtgaa atttttgata
    11641 ttaacgctgg cgatacactt ttagttcttg gcgacgaaga acaaggaata gcgattgtca
    11701 aggcggatgt gatgaaagaa ctggcagtaa aaattttaaa agggcttggt agtttgagtg
    11761 gtgggtttaa tgatgaaaaa tagcatttct ctattcttac cattagcctt attgctggat
    11821 ttaactattc cttttttcct cgctccgttt tataaaggtt atagtcattc tatgcaggta
    11881 atgagcgtgc ttggaaataa caaatctcca ctacatttta tatacaactt atggctagtt
    11941 ttatgcggta tcattctttt aatgagcggt tttaaggttt atgaaattgt ttcgcagaaa
    12001 tcaaaaaaca tagctgttat tttgtttata gtcatattga tatatgcgat aggtggctgt
    12061 attctctcag ggattttttc ggtaggagaa acaaaatctc tcaatactat ccctgaaaaa
    12121 atacatggtt acggggcggt tcttagattt atatgtttaa cttttgctcc gcttttgatt
    12181 agcttatatt ggtttcggat tgaggaaata ccacttgcac tattttcaat gatttgtttt
    12241 cttctagcat ttatcttttt tacgattttt gttatggcag ataaaccaaa ctacaaagat
    12301 acaatcattg cttttgaggg cttatggcaa cgattatcac tactgtttat gtatttacct
    12361 ttggggtgtt taatattact ggaaaaataa attattagga gtgaactcta tgataggact
    12421 aaaaaaacga gatattaaaa aagccttgaa agctggtgca aaagctggcg gtatgtcatt
    12481 ggctgatgtt cgggcggata ttgaagcgac aattgatgaa gctatgaaca gcaccgaccc
    12541 agaggtgcag gcaaatttca aaaaaaattt tggaaataaa cgccctgcac cagaagaata
    12601 tatttatata attacaaaga aaacagttaa agggctgaaa gtatgaaaat agaacgtctg
    12661 ataggaataa tcttttattt aataaataga gagtgtgtaa caacaaatga attagcacaa
    12721 aagtttgaag tatctcgcag aacgatttta cgagatattg atacactaac tcttgctggt
    12781 ataccgattt attctgagtt aggtgtaaat ggtggctata taataaataa agattttaag
    12841 gttgatgaaa aaattattga cagcaacaat aaagaatata ttttgcttgc tttaaatagt
    12901 ctaagaactg tttacggaaa taaaaagatc attgaaactt acgaaaaaat gaaacatctt
    12961 tataacgatg tcaatattaa tgcacttcca gatgttgatt tctctgttgt aactgaaagt
    13021 ccacaaatta taaggtgcgt agacttaatc aataaagcaa caaaagaaca gcgaactata
    13081 aaatttatat atactaattc agcaggacat acaaaaaaag tttttctaaa cccattacat
    13141 acatattata ggtggtactc ctggtatgtt tttggatatg acacctctaa aaatgattat
    13201 cgcatgttca aagtggtaag aatgaaaaat atttctttaa caaaagaact ttttcaaacc
    13261 aaagataata tagctgacct tttatctgat tttgaaaaaa gacgcaacca gacaaatatt
    13321 acggttactc tatcttataa acaagaaata gacaccttag ttaatgaata ctttaaagga
    13381 aaaattgttg aaactgtcaa tgactgtttt ataagagaaa ttgagattaa agaaaatgat
    13441 tttatgacat tctcaatcct tttaggactt tgcgataaag taaaaatatt atcccctcaa
    13501 aaatacaaag aaaaggtatt aactcatatc agaaaaataa agaaaaattt cacttaatag
    13561 tgacatacat ttgtcactat tcctgctata taattttctt atatcgaaaa tatgaggaga
    13621 tgtgaaaatg aaaaaagtat atgtatattt attagacgac atggcagaat gcgaaattgg
    13681 ttatatttta caagcattca gtatggaaaa attattaaaa aatggaacta aagaatttga
    13741 agtaaagaca gttggtttta ataaagaacc cgtcttaact ttaggaggat taacaattgt
    13801 tccagattat agtatatcag aagttgactt caatgctagt acagctctgc ttttaccggg
    13861 ttcttccact tggggaagta aagaaaatca agaaatattg gaaaaagcac aagaatgttt
    13921 aaacaaaaat attttagtag gtgctatttg tggggcaaca ctcgcacttg ctgattatgg
    13981 tatattagat aaatttaaac acacaagtaa ttctctagaa tatctaaatt tcttttcaag
    14041 aaactatgca ggacagagct tgtacgtttg ttctaattcc gttttagaca gaaatttagt
    14101 aacagcaaat tcagcaggtt cattagaatg gacgaaaaat attctaaatt accttaatgt
    14161 gtattctgac aataaaatta ctgcttttta caattattac tcgacaggaa acgcaaaata
    14221 ctatgatgaa cttttaaaat aaaggtggtg tttctgtaat gaaacaatca tgggtattat
    14281 gcccatctgc aataacaaga cacgaacaaa aatacgggta gatacggaac tgataagttt
    14341 ccctttgttc tgcccaaagt gcaagcatga aactttaatc aatataaagc atggaaatgt
    14401 aacgactatt aaagagccag acgctaagac gcagagccga tagtttatag gttattgtaa
    14461 cttatagatt gttggctctt atttgttacc tgcggagcgg atcagcaaat aagttttgga
    14521 atataatgga aaaacattta tataaagaaa agaagaaaca tgaaaatatt agtaagtgcc
    14581 tgccttttac aatataaata tactaaaaaa atcgtaaaaa gatcctcaat aattcatata
    14641 tttttactaa tgaaagatca atatgacata ccaaagcgtt atttgcttct attcaaaaga
    14701 gtgtcaattt cttcttctga taatttttgt ttgaaattaa acacattatc tacagctgtt
    14761 cctaaaagtc ttgccaaagg gcttagtaat gaaatatcag gatatgtatt gtttgtttcc
    14821 cattttgaca ccgaagaagt agttattcca agagcatttg caacttgttc ttgtgttaga
    14881 ccttttattt ttcttagttt ctgaataata tcaccgataa ataatttcat tttgtgacat
    14941 ctcctttact taatgaaaat attatcagat aaaaactttt tttaacaatg caggtatcgc
    15001 agtatgaact tgtgttacat aaggatagta actggaagat tgtaggataa atcaaaaaat
    15061 gccgtagctt tattttgcat ttattgcaag ttacacgcta cggctagatt aaaatataat
    15121 attttgaaat cttcataaaa tctttaattt tgctttttat actttaacta aaaaataagg
    15181 aaagggtatg aacaattatg aaagcaattt taaaaacaaa taacctatgt aaagatttca
    15241 aaaagcaaaa ggcagtgaat aatgtatcta taactgtccg tgaaaattca atatatgggc
    15301 tactcggtcc aaatggtgct ggaaaatcta ccactttgaa aatgattacg ggtatgctcc
    15361 gtcctactag cggtaaagtt ctttttaatg gacatgaatg gaacagaaaa gatttagaac
    15421 agataggagc tttgattgaa actcctccac tttatgaaaa tctttccgca gtagaaaatt
    15481 tagaagtccg tgcaaaatta ctaaatattc ctaaaacccg aatagataaa gttttaaaaa
    15541 tggtagattt gcaaaataca ggaaggaaaa aagctggaca attttcaatg ggaatgaaac
    15601 aacgtttggg aatagctatc gctttgttga atagtcccaa attgttgatt ttagatgagc
    15661 cgacaaatgg attagaccct atcggcatac aagaattacg ttctcttatt cgttcttttc
    15721 cctctaaagg gattactgtt attctatcca gtcatattct atcagaggtt cagcttatcg
    15781 ctgatgatat tggtatcatt tcaaatggta ttttaggata tgaaggacag atgaataaag
    15841 atgaaaattt agaaaatctg tttgtagaag ttgtaaggaa atcacaggag gaacgataaa
    15901 atgaaaaatt atatatcttc tgaatattta aaacataaaa atacattcgc aaaaaaatta
    15961 gtatggttag ctcctatact aactttatgt ctgaatatac ttgctcctat gtggtatcaa
    16021 caaaattcgt ataactggtg gtatgtattc ttatatcctg gttgtttaac gctattatct
    16081 attttgataa ttcaaagaga taacggcaaa ttaaaattta gagcaatttt gtcattgcct
    16141 gttgacttaa aaaaagtatg gtatgcaaaa ataatcactt gtataatata cattgtactt
    16201 gcaaatacaa ttttgatgtt atgcaatgtt gcaggtggtt ttatcattca acacattttt
    16261 gatataccta tgacaattag cccaatgcag gcattgttcg gaacactttg cattattctg
    16321 gcaagtatat ggaatattcc attatgcttg tggtttacaa aaaaagtggg tgtatttgcc
    16381 actctcatat taaatgtcgg aatgagcttt atgttgggaa cattaggggc aaatacaagt
    16441 ttttggttca tatgtcctta tagctgggtt actcgcctta tggttcccac attaggaata
    16501 ttaccgaatg gagaatcagt aacaggaagt gctttatcaa ctcctttacc gttggttatt
    16561 atgacaatct gcttgtcatt gcttttacta tatacaattt caaagtcaac ggcaaaatct
    16621 tttataaagc aggaggttct ttaatgttta gtattatcag atgttttcaa gcagaatggc
    16681 ttaaaagcag acatacacca ttacttttag tccatgtgct ttttccgtta ataggagctg
    16741 gtgtttttgc aggatatttt catatatccg gttggagtga gatgacaaat gtaacgacct
    16801 ttttagaggt gttagcaatt atttttccat ttgttattgg tatcgttgta ggtatgatag
    16861 tcgaactaga aaatggagca ggtcattttc agttaatctt aggtacgatc cgttcacgta
    16921 tagcggtata tatcggaaaa ttagtatatc ttatcgtttt agcaaccttt gcaacaatac
    16981 tttgtatttt cttgtttgcc gtactctatc cagttatgcc attttcgttt tatataaaac
    17041 ctcttattat gttgatattg tctgtaatac cactttatct tatttccata cctgttggtt
    17101 tcagtttagg caaaagtgta tctatgggca taggtattgt aggaagttta ttatctgcat
    17161 tgctagttac aggacttgga gattttatct ggaaattcct gccgtggggc tggggtgtac
    17221 gatttatgga ttattgtgtt ttaagatatg ttaatgaggg gcagtttcat tctaacttac
    17281 ccgaatttca actaggtttt atactcatgc tggtttttac agtagtgctg tttatcataa
    17341 gcttagtgtg gtttcagaga tgggaaggag taaaagaaaa tgagtaaaaa aatgatgata
    17401 ttgctgtttg ctgttattat atgtttatct cttgcaggct gttctttaca ggacaaaatc
    17461 ggggactatt ccagagagca atgctatctg aataaggaaa atgtgacgca attttcctat
    17521 aagggaaatg actacacgat tttagcggat actgtctcta atagtggact cggggaatgg
    17581 attgggtata tccgacaact tgcggctgta gacgaatcag gaaaaatatt acttcaagaa
    17641 aatctcaaaa ctgctacctt tcagacgttg gcagatttag cagatttagt ggataaagcc
    17701 cctaatgacg cctatattat tccattctta aatgtgtatg cagctcctaa cgctgacgac
    17761 tatctgattg tagatataaa tggagggtat cacaaagctg ttatagatga aaatatcaaa
    17821 ggcacagata cagtttttga ctttaaggat atagagcaat ctatgagtgg aaagtttgaa
    17881 atcaatcctc aaaatgcaac gcagcttctt tgtgatggga cggtttatca agtaacttct
    17941 gatactgtat caaataatga attgggaagt tacattggta ttcttgcgga gaatgtcata
    18001 tttaatgcag aaacaaaaat tcctttgtca aaggaggaac taagaaaaat tgactggtat
    18061 gggaaaaacg ccggacaaca tcgggaacaa tggatttaca aagatattta tgaaattcat
    18121 ggtacagaaa agaccgaagc cgttgcagta cagataaatg accgctacta tattgcaaag
    18181 cgtcaatgac cgatttttgt ttttctgcct ttatggatta tgatatacta aaaaagctag
    18241 gaggcgatac tatggcaaca atccttatga ttgatgatga acgacctatt ttagaacttg
    18301 tgaaaaatgg actgcaaaag gacggacatt tagttacagt ctatacgtcc gccgcacagg
    18361 ttcctcttga taaactgaac agatatgact tgattatact ggacattatg atgccggata
    18421 tagacgggtt ttcttattgt gataaaatcc gttcattagt ggactgtcct atcctctttc
    18481 tgactgctaa gacaatggaa aatgatatta cattcggtct tggattagga gcagatgact
    18541 atttgacaaa accattccgt attacagaat tacgggcaag agtaaatgcc cacttacgcc
    18601 gcgaacaccg agaacgacat atagctctta catttgaccg gataaaaatt gatttatctg
    18661 caaaagaatt acgggtagat aatataccag tgcctttaac gaaaagtgaa tatctgattt
    18721 gtgagtatct tgctagtaac aaaggacagg ttttttcaaa ggaacaaata tacgaagcta
    18781 cattttccct ttatggagaa agcgatagtt ctacaatctc gacacatata aaaaatataa
    18841 ggtcaaagtt tgagaaaacg ggtgttcagc caatcgtaac agtttggggg ataggttata
    18901 gatgggaata aaaatgaaaa catcacttac aagaactttt tataaatttc ttgtttgtct
    18961 acttggcgga ctatgcttgt ccgttgtcgt cccttttgcc tgtatgatgt ttgcttccag
    19021 tatagggctt gtaacacttg cagataataa cgaacggcaa acgaaagctt tagtaccgat
    19081 actaactgca acaccaaatg tatcagatat acattttcct actggaacta aatttcttcg
    19141 attaactaaa aactatggat acatagatac gaatatgagt gaagaagaaa aagaaaaggc
    19201 tttaatgttt gctaaaacgg ggttcatgga tacaagtggt aaaacacagt ttattttcat
    19261 tactcgagat gatgaatata ttgttttaca gtatgttata ggttctcaat atttaaatgc
    19321 acaatttaat cagtatttac cgtccccaga aattatgatg atttctttaa tcactataag
    19381 cagcttaggg gtatgtgttt atctgactgt tcattttgct aaagagctga gaaaagaatt
    19441 gacacctatt ttagaagcta ccaaagaaat tgagaataac aacttagatt ttactatcgg
    19501 acattctaaa atattggaat ttgaaaacgt gattatatcc tttgatagta tgcgaaatag
    19561 tttaaaaggg tcattagaaa aacagtggcg aagtgaaaaa gcacaaagag aacagattgc
    19621 atctttagct catgatttga aaactccatt aactgttatg caggggaaca ttgacctatt
    19681 agatgaaaca gaacttgacg aagaacaaaa gctatattta tcgtatgcaa tgagtagctc
    19741 tgagcagatg aaacagtata taaaaatttt gattgatata tctaaagcat ctgctggcta
    19801 tcaattacaa atggaagatg taaattttcc agagttttgg aaacatgtct tatctcaaac
    19861 tgaaatcatc tgcaaagata aaggtattgt tattcagcag acacagcata atttcccaca
    19921 gaatataact ggggatagaa tgttactgga acgagcattg atgaatttgg tttcaaattc
    19981 tttagagtac tcgcctgaaa actctacatt atatatagat gtagatagca aggacaatta
    20041 cttgaatatc tgtattacag actgtggctg tggcttttcg caagaggcat tggatcacgc
    20101 aaaagaacgt ttctatatgg cagaccaaag taggagctct aaactgcatt atggaatggg
    20161 tttatatatt gttgatatca ttataaaaca gcataaagga aaacttctgc tagataattc
    20221 cacagaaacc aaaggtgcca aagttactat aaagttacca ttataagaga acggcttatt
    20281 tgtcgttctt ttttcatatc tttaagaaat cttcaaaatt aggttgtatt ctttgtttga
    20341 aggtaatggt tatatgctat tatacggggg attgtgtatg aagtgtttta agcaatttac
    20401 caaaaactca agaacaactt aacagcaatc catgcgctaa tacaactgaa tattttaata
    20461 tcttagctcg tacagactat atgttttgtg cgggcttttt ttgtacccga aaaaattttc
    20521 taactttttt cggcttcagc ttagcaaatc acaccttgct gtacagatat ggtgcggaaa
    20581 gagggataaa cacttttcaa gtcatagctg gaaagggggt gagattatga agccgtctga
    20641 tttccagaaa acaatacaat gtcaatttga atgtaagtta aagaaagtta caaaaggcat
    20701 tgtaagaaat tatcagaaag aattaaaacg gagaaaaaat aaggaaattc ctttttgtga
    20761 acttccagaa ctagtgattg aaaaattatc cgttttagac gattacgaca gcgactatac
    20821 attattcaat gtatgtggca acgatattag tattcttgat gatgaattgg cagaagcctt
    20881 gaaaaagctg tctgaccgta accgtgagaa cttactcatg tactacttct tagaaatgag
    20941 cgataccgag attgctaaat tgcagaatat ttccagaagt ggagtatttc aaaatcggta
    21001 taactcattg gaacttatga aaaaaatact aaaaggagaa aagtaagaaa tgaagcacat
    21061 ttttaagaaa ccgtcatttt atctgatttc gtcagctacg gacggagacg aaaaagcgat
    21121 tgaaaagata ctggcaattt atgacccgta catatcaaaa tgttgtttgc gttcactcta
    21181 tgatgaatac ggtaatgttt atattgccgt ggatatggaa ttaaaaggac gtatcagaga
    21241 agcccttatc aaaatgattt tggactttga tataccttta gaaaccgagg aagaataaca
    21301 agcagtaacc gcagagcatg atttgttcaa tcccctcttt cctgctctgc tccctgcttt
    21361 tacatgaaag caacacgctt tcgccacagc tctttgacaa ctgaataaag cagacagata
    21421 cgtttgtgag atagcgagcc acggggactg tacgccacga ccttttcaaa agaaagcgag
    21481 cgacccacgc agtgatccga gagcgactgt tggaaaagtc gttttgccac gacctatcct
    21541 cacagaataa tgatacgtcc gcatggtgcg gttctgccca caagaatggg aatagttgag
    21601 atactaacgg agctttccaa agccgtctgc ctgcttgtaa aaaatgacac acagtaaagg
    21661 gggtgcatag attatgaaca atactgatgt gcctatctgg gaaaaatata cgctgacgat
    21721 tgaagaagcg tctaaatact tccgcattgg cgaaaagaaa ttgcgtaaat tagccgaaga
    21781 aaatattgac gctggctggg ttatcgtgaa cggtaatcgt attcagatta agcgaaaaca
    21841 atttgaaaaa atcatagata cattggacga aatctaatgt cattgagccg tagatatggt
    21901 ataataaggt atagcgtatc acggctcatt ccatgacgga aaggagcttt acactatgtc
    21961 taatgtaaaa cgaaaagaca gtaaaaatcg caatttgcgt aatggagaga gccagcgaaa
    22021 agacggaaga tacgtttata aatataccga tatatacgga aagccacagt ttatctattc
    22081 ttggaaactt gtaccgacag acaagacacc tgctggaaag cgtgatgata tatcgttgag
    22141 ggaaaaagaa gcacagataa aaaaagacct taacgacggt atcgacacag tcggcggtaa
    22201 aatgacagtc tgccagctct acgagaaaaa gaacagtcaa agaaagaaca tcaagagggc
    22261 tactgaaaaa ggacgacagt atcttatgaa cgctctaaaa aatgacctgt tgggtatgag
    22321 agcgattgat accgtcaaac agtcagacgc taaagaatgg gctatcagaa tgagtgaaaa
    22381 aggatatgcc tataaaacga ttgataacta taagcgttcc ttgaaagcgt cattttatat
    22441 ggcgatacaa gacgactgta tcagaaagaa cccgtttgaa tttaagctaa gtgatgttct
    22501 ggaagatgat acggagcaga aagttatcct tacaccagag caggaaaaac acctgcttgc
    22561 ctttatggaa acggacaaga tttacagtaa gtattatgat gaggttgtgc ttctgctgga
    22621 aacgggactt cgtatttctg aattttgcgg attgacgacg catattgata tgcagaacag
    22681 aatactcaat atagaccacc agttattgaa agatagcgaa atgggttact atattgaaac
    22741 gccaaagact aaaaacggaa aacgtgaact tccattgaca gaacgggctt atcaagcaat
    22801 ccaaagaata ctagagaaca gaggaaaggc acaaccgttg attgtaggtg gttacagcaa
    22861 tttcctattc ttaaaccgtg agggcttgcc taaagttgca ggaaactatg agggtatggt
    22921 gcgaggattg attaagaaat ataacaagta tcacacggat aagttaccga acatcacacc
    22981 acattcattc agacatactt attgtacgaa tatggcaaat agaggaatga acccaaacac
    23041 cctgcaatat ctcatgggac acgctaacat aaccatgaca cttggctatt acgcacacgg
    23101 tacatttcaa tctgcaaaag cggaactgga aagactggct tgttaatatc ggagcttata
    23161 tttactactc atttactact tttggttgca ttttcatgct ggtaaatgcc agcttatgca
    23221 aggtatcttc caaaaagaaa ataccgataa agccctaaaa tacgggatat atcggcattt
    23281 accaacttat gaaaagataa tcagaaattt agtaagtttt tatctcaaaa atttactcct
    23341 gttgtaaata atatgactaa gaatatatgg gtacaatcag ttcaatcaac tataatgaaa
    23401 gttttgccaa tggtatttgt aggctcatta gtaactatag tttctgtctt gaagaattac
    23461 ataagctttt tacctgattt aagtccaata aatcaatata catttggact tttaggatta
    23521 tttatagcat ttttattgcc tatggaaata atgaagaata agaaatttga aagtatgagt
    23581 gtagtcgctg gtcttgcagg tgctggatta atgttaatga tgattcgtcc agagattacg
    23641 aatgaaggag ctatatttaa cttcaacagg tttggaggag aaggaatgct tgtagcacta
    23701 gttgcaggat tattctccgg attaataatg tctttatttg ggtctttctc attttttggt
    23761 gaagattcag cacttcctga ttttgtaagt aagtggtttg atagtatgtt acctatagca
    23821 gtaataatgt ttataggttg gggactaatt tatggactta actttgatat gtttgaaata
    23881 atagttagga tatttaaacc agtatcagat atagcacaaa cattgcctgg atttgtacta
    23941 ttaaacttct tagtagtatt tttatattct ttaggtataa gtggatgggt attatcccca
    24001 ataacttatg caattcagct tagtgcaatt gcagctaatg cagcagctgt agcatcagga
    24061 gggcttgcaa ctaatatata tacttatgaa gttgttcaaa caggttggat aactttaggt
    24121 gggtggggag caacattacc actagtgcta atgatgtgtt tatctagagc tggaagaata
    24181 aaagcaatcg gaagagcctg tatcgttcct tctataatga acataaatga accagtaatg
    24241 tttggagctc cagtagcatg gaacccattt ttaatgttac ctatgtggat aggaacagta
    24301 gtaatatcaa taactactta cttatcaatg agtatgggat ttgtgacaat tccgagtaag
    24361 gtgtttaatt tatggtatgt tccttttggt atatctactt ttatggtaaa tcaagatgtt
    24421 agagggctta ttgttttagc aataaatata gtattgctat tcatgatatg gtatccattt
    24481 tttaaagctt atgaaaaaga agaactaaaa ttagagagtc aagaagctta aatttagatg
    24541 ttgaaaggaa attataaata tgacaactga agaacatgta atagatgaag aattagtaga
    24601 agtagcaatg caaataatac ttcgtgcagg tgaggctaga acagaaatta aacatgctct
    24661 taatgattta gaaaggtttg attataaaaa tgctgattta aaactagcta aagcaaaaga
    24721 gtttatgaca gaagcacata gagctcaaac taacattata caaggggaag ctagtggaga
    24781 aaaaagagca cattcacttt tatttgcaca tgctcaagat actcttatga ccatattcag
    24841 cgagttaaat ataactatgt ctttagtagg aatagtaaag tctatagaat ataaggtaaa
    24901 taatagtaaa tgatttaaat aaataaaata aggagtgatt aaattgagta agtttcctaa
    24961 agattttttg tgaggtggag ctgtagcagc taatcaatta gaaggtgctt ggaatgaagg
    25021 tggcaaaggg tggtgcttag ctgatgtaca tatgtatgat aaagatagag atatatctaa
    25081 acattacgag tctgatatga ctgtagataa aataaagttt gctatgaaag ataataatgg
    25141 gtattatcct aagcgtaatg gaatagactt ttatcataca tacaaagaag atttaaagct
    25201 tttgaaagaa ctagggttaa agtgttttag attttcaata aattgggcta gaatatttcc
    25261 aaatggagat gatttagagc ctaatgaaga gggattaaag ttttatgata atcttataaa
    25321 tgaagtaata gataatggta tggaacctat tgtaacacta ctccattatg aaactccttt
    25381 gaatttagta ttaaaatata aaggatggtc taatagaaaa gtagtggatt tctttgttag
    25441 atatgcagaa ttaatattaa atagatacaa agataaagtt aaatattgga tagttataaa
    25501 tcaaataaat ttaatgtatc atgaatcatt taattcagtt gcattttgtg tagaccaggt
    25561 agatgatgta gaaaaggcca aatatcaagc tattcatcat caattcatgg ctacagcttt
    25621 aactaagaag cttggaaaag aaataaattc agatatgatg atagggatga tgatggcaga
    25681 ttgtacatct tatccagaag attgcgaccc tgaaaatgtg gtgtttgctt taaaaagaaa
    25741 tagaatacaa tattttttca ctgatgtaag ttttagggga gagtatccag tctatattaa
    25801 gagatttttc aaagaaaata acataaatat agttatggag gaaggcgatg ataaaatcct
    25861 aaaagaaaat actatggatt ttctagctct atcatattat tactctacaa cagtatcagc
    25921 aagtaaaaat accatgaatc caattgataa tgtaaaaaat cgatacttaa aagcaaatcc
    25981 ttgggggtgg gcaattgacc caaaaggatt atacaatact ttatctcaat attatgatag
    26041 ataccaaaag ccaataatga tagctgaaaa tggatttgga atgcatgata aatttgaaga
    26101 tggaaaagtt catgatagtt atagaataga ttatttagga aaacatatag aacaaatagg
    26161 tgaagctata gctgatggaa ccgaaatatt agcttattgt atgtggtcgc caatagatat
    26221 agtaagttgt tcatctcaag aaatggaaaa aagatacgga tttatatatg tagatataga
    26281 taacaatgga aatggaagta aaaaacgtta taaaaaagat agtttttatt ggtatcaaaa
    26341 tgtaataaga actaatggag aagaattaaa agaaaagtaa atagttttta aatataaata
    26401 atccctctta taaatactat gttattaata cagttttata agagggatta taattataaa
    26461 taaggtggtt tattaattat gaatgtcata aaaaaattta tatttgtaat tttaattata
    26521 aatatactgg gatgtggtat atctcttata cttcagtcaa gccttggtag tgatgcaatt
    26581 acacttttaa atgagggtat acatataaag ttaggtataa gttatacaat agcagggcta
    26641 atatataatg gaactctatt acttatagca ttgctattga ataaaaaaag tttgggatta
    26701 ggaagctgtg tttatgtact agtagcagga atatttatag atttgtatat atacttatta
    26761 ttagatttta atataagtag tttagggatt atcattagaa taatgagctt tataattgga
    26821 catatactta tgtgtagtgg atttgcaatg cttataaaat ttgacattgg aatgagccct
    26881 ttagatgcta tattaatcta tattgagggc aaatttaagt atccatataa agttttaaaa
    26941 actatagtag atgtaatatt tttaatttta ggtgtatatt taggcggaaa tttagggttt
    27001 ggaactatat tttcaatatt atgtactgga actacaattt caattgttag taagatgatt
    27061 tctgttaata aaaaaaggta ttactcaaat atataaattg ataagtgaga gtatggataa
    27121 tgtttatgaa tcattttcaa taattcgagt taatttgagg tcgtaaaaat tggagttact
    27181 cagtttcagt aattgagtaa ctccaatttt tattacaaaa tactctaata attacataat
    27241 taattgaagc ataaaattat tatattataa atttacatat atagacattg actaaatctt
    27301 aattgtatat aaaaataagt agttaagagg taatatatta tattactcga taaatttaga
    27361 caaataaaac ttatataagg ggagataaca tatgataatt gagctaaaca aaagtaattc
    27421 tgaaaatcta acgaagacgg aacttcaaat aattaaattt ataaatgaaa atgaatcaat
    27481 attaccagaa ttatctatag tagagattgc tcatgaaaca tattcatctc cagcaaccgt
    27541 ttcaagagca attagaaaat gcggtttaaa tggattcaat gaacttaggt atagattaac
    27601 agtaaaagat gaaaatgatg atatacaaaa tatgggggat ataataaata aatcctttat
    27661 agaagcacag agagttatag aacaaatatc tttaactaca ctaatcaata taataaaaac
    27721 tataaataaa tctagtaaga tatatatttt ggcaagaggg cttactgaat atgtagcaaa
    27781 agagtttagt cttaaattgc aattacttga ttttaatgtt gtttttataa atgaccccaa
    27841 cataatgaaa ataaaatcta aatcaataaa aaaagacgaa cttataatta tattttcatt
    27901 gaatggaaat accatagagc ttgtagaatc agcacagaat gccaatttag ttggagctaa
    27961 ggttataact tgctgttgta gtgaaagtgc aaaacttata aagtattcgg atttatatat
    28021 tgtaggatac aaacatgaac atatatcaat ttctgaatat gaagtatctt ccagattgcc
    28081 cttgtatatt atatctagga taattataga ttatatggtc agatataata attctgaaaa
    28141 ttaaattgaa aagtctatga aaatcgattt tctatttgtg aaaagatttt catagacttt
    28201 tttattataa tgaaaacata cacagcacaa gcaaggggga agacaatgat atatacatta
    28261 actacgaatc ctgcaataga tatgaatata tctactaatg ggataaagag aaaattagta
    28321 aatcgtacat caaatgctgt atattctcct aatggaaagg gattaaatgt aacttttgta
    28381 ctaggtcatt atggtataga atctaagata ttagggtttt ttggaggatt ttcaggaaaa
    28441 tatatagttg aagaaagcga aaagagagga tttgacgtat tacctacttg ggtagaagat
    28501 acaactcgta taaatatatt tttaaatgat ggaagtgatg aatttaaatt tgtaaactct
    28561 gggtcatatg taaatgaaaa acaaaaacta gatatgctag ataagataca gtcattagca
    28621 gatataagtt acttatcaat aagtggaagt ctacctcctg gtattgatga taattactat
    28681 gaggatatat ttaaaatttg taaaaataaa agtataaaga caatactcga tataagttca
    28741 ccaaaattaa aggagttact acagtataat ccatatttga taaaaccaaa tgatgaagaa
    28801 atcaaagata tatttggaat tattgtaaga gatgaagaag atattaagga tgtacttaaa
    28861 ttactgcatt taaaaggtgc tcaaaatata ttgctaactt taggagaaaa aggttcttat
    28921 ttttataatg gaaaatcaat ttattatgct agtgctcaac cagttacagt agtcagctct
    28981 gcatgtgctg gtgattctgc attagctgct tttttaagta tatggttgga aaatccagaa
    29041 catattgaag aagcattaaa aaggtcagca gcaacaggag caagtgtggc tgaaagtaat
    29101 ggtataggta atttggagaa tgtggaagat tatataaaaa atataagagt cagaaaggtg
    29161 gtataaaaat gggaaagaaa atgattctag gggtaacagg ttgtccaact ggaatagctc
    29221 atacttttat ggcagaagaa gcattaaaaa attcagccag ggaattaggg tgtgacataa
    29281 aagttgaaac gaatggagct ataggtgtag aaaataagtt aacagcaaaa gatatcgaaa
    29341 tggcagatgc aataatagtt gcttgtgata aaaatgttga tatggacaga ttcaatggaa
    29401 agccagtaat agaagtacct gtcaaagaag gtatacataa agctaatgaa cttatacaaa
    29461 ggtgtattga tggaaaagta gctattagaa atggaaactt atcaagcagt aaaattgcag
    29521 aagaaggaaa tttatcgttt ggtcaaaagt tgtataaaga tttaatgaat ggtgtatctc
    29581 acatgttacc tttagtagta gcaggtggag tacttactgc tatatcattt ttatggggga
    29641 tttattcatt tgacccaaat tcagaacaat ataatcaaat agcagctaca ttaaaatctg
    29701 ttggtggata ttctatgaac ttgatggttc ctgtattggc agcatttata gctcaatcta
    29761 tatcgggaag agctggtatg ttggcaggac ttgttggagg aatgatatca tttgatacag
    29821 gttcgggttt tttaggtgga atcatatcag gatttttagc aggttactta gttaaactat
    29881 ttgtacattt gttaagaaaa ttaccacgac aattagaagg tcttaaatcg atttttataa
    29941 ttcctattgt cagcgtaggt cttgtgggtg tagcaatgtt gttacttggt gggccatgtt
    30001 cagcacttaa caatgctatg atgaactttc tatcggggtt acaaaattca agccctataa
    30061 ttttgggaat agtaatagga tgtatgtcag catttgatat gggaggtcca gtaaataaag
    30121 cagcatatgt aactggaact atgcttctag gacagggaaa ttatttattt atggcaggtg
    30181 tatctgcagc ttgtataaca ccaccattgg taatagcaat agcatctact ttatttaaaa
    30241 atagatttac agaagaagat agagcagcag gattaatcaa ttatatacta ggtagtactc
    30301 atataacaga gggggcaata ccttttgcag caaaaaatcc attaaaagtg ttgccagtac
    30361 ttatgattgc atcatcaata tcatcaattt taacttatat aatgaaaata gaagtaccag
    30421 ctccacatgg agggtttttg gttctaggat tagtcaataa gccactttta tgggtagggt
    30481 gtatatttgt aggttcatta gtaggtgctg tactttatat gatggttaca cctaaagtag
    30541 taaatgagaa aaatactata gaaaatacaa atgcaacaaa agatgaacat aaaaatgaaa
    30601 gtgcagaatc ggaaaacgta tctaataata tctctttata tagcgaagaa acagtaattt
    30661 tagatgttaa gggaaaaaat aaaacagatg taattgatga aatgattgga atacttgata
    30721 aatctggtgt tcttttagat aaaaagaagt ttaaagaaga aatccttaaa agagaagaaa
    30781 tatcatctac aggttttggt atggggatag cgataccaca tgcaaaaaca gatgcagtaa
    30841 aggtaccacg tgtagcagta ggtgtatcaa aagaagggtt tgactttgaa tcagaagatg
    30901 gaagcttggc acatattata tttatgatag cagctacaga taatggtgat aatttacact
    30961 taaaaacatt atctcaacta tcttcaaaat taatgaatga agaattttta aatgagttat
    31021 taaatagcaa aacaagtaga gaaatagtat caaaattaaa taacgaagaa atcaaatcag
    31081 tataaacata taggaggaga aattatggca ttagtgacaa ctaaacaaat attattagat
    31141 gcacaagaag ggcattatgc agtaggtgca tttaatgttg aaaatatgga gatggttatg
    31201 gcagtaatag aggctgctga agaacttaaa tcaccagtaa tattgcaaac aacaccttca
    31261 acaataaagt atgcaggact agattattat ttagcgaatg taaaggtagc atcagagaag
    31321 gcaagtgttc cagtagcaat gcacttagac catggttcaa gctttggact agctatgcag
    31381 gcacttagag ttggatatac ttctattatg attgatggtt ctcatgaaag ttacgaagat
    31441 aatatttcaa tatctaaatc tgttgtagat gcatgtatgc cttcagatat acctgttgaa
    31501 gctgaattag gtaaagttgg aggtaaggaa gacgatttag atggtggtga tgggggagca
    31561 tatacagacc cattagaagc aaaagagttt gtagagagaa caggggtatc atctttggca
    31621 gtagcaatag gtacggcaca tgggctatat aagggagagc caaagcttga tttagataga
    31681 ttatctgaaa ttagagaggt tgtatcagtt ccattagtat tacatggtgg gtctggaata
    31741 ccagatgagg ttattaagga gtctataaaa agagggattt gcaaggttaa ttatgcaaca
    31801 gaattaagga ttgcttatag caatggggtt aaggatgtgc ttaattctga cccagaggta
    31861 attgacccta agaaatatgg taaaaaaggt ttagagtcag ttaaagattt tgttaagagt
    31921 aggatggaag tttgtggatg tgtaggaaaa gcaatagaga tagtgaagtg ttaataataa
    31981 agggagagaa taagtaagat taaattaagg ggtgtctcaa aatgaacttt ttagttcatg
    32041 aggcactctt ttttgtatga aattaaatat attttcatca ttccgaaaat aaaaaagaag
    32101 attatatcta ttttgagaca gcatcttatt ttattttttg attaagattg taattgagaa
    32161 atttgaaata aggaagatga tataattaaa taaaaaattt ctttctaagt ttaaattaat
    32221 attaaaaagt tataatatgg taagagttat ccaggtagat gactcctatg ctggatatat
    32281 gataaaataa ttttgtatgg gatgtataat aatcataaaa taagagtatt gcattagttt
    32341 tttaactaaa acatgtaaat aggaaaaata atttaataac tgtgtagtaa aattttgaat
    32401 ttaatataag acactaatta ttaaggcgta cgactaaaat atatatttaa attattttat
    32461 gaacactgga gggaaagtca tatatgatta caggagaatt aaggagcaaa gtagacaaga
    32521 tttgggagac attttggact ggtggattaa ctaatccatt agaagttata gaacaattta
    32581 catatctttt atttattaaa ggattggatg aagtagaaac aaaaaatgaa gcagaagcaa
    32641 ctcttttggg agtttctttt gaaaggatat ttgatgatga acatcaacat ttaagatgga
    32701 gtaagttttc taatgaagga aattcagaaa aaatgtatga aatagtacaa aatgaagtgt
    32761 ttccttttat aaaaaaatta catggaaata aagactcagc ttatgcaaag tacatgggag
    32821 atgcaatatt taaaattcct acgcctctta tgttatctaa aatagttgat ggtattagta
    32881 atttagaatt aagtaaggat agagatacaa agggagattt gtatgagtat ttattatcca
    32941 aagtggctac agcaggtact aatggtcaat ttaggactcc aagacacata atagatatga
    33001 tagtaagact tattaaacca acaccagaag atattatagt agacccggca gcaggttcgg
    33061 caggtttttt agtatcatca caacaatact taagggataa tcattcaagt cttttcttag
    33121 tgcaagggct gaaggagcat tttaataatg gtatgttcta tggatttgat atggatagaa
    33181 ctatgcttag aataggtgct atgaatatga tgttgcatgg cgttgacaat cctaatatag
    33241 agtataagga ttctttatcg gaggttaata cagataaaga taagtttaca ttggtgttag
    33301 caaatcctcc ttttaaggga agtttagatt atgaagcagt gtcagcagat ttattaaaag
    33361 tgactaagac aaagaaaaca gagttattat tcttggcact ttttttaaga gttttaaaaa
    33421 ttggtggaag atgcgcatgt atagtgccag atggagtatt gtttggttca accggtggac
    33481 ataaatcgat aagaaaagaa atagttgata atcataaatt ggaagcgata atatcaatgc
    33541 caagtggtgt atttaagcca tatgcaggag tatctacagc tataatgata tttactaaaa
    33601 caggaacagg tggaaccgat aaggtatggt tctatgatat gaaagctgat ggttattctt
    33661 tggatgataa gagaaatgag ataaaggata gtgatataga ggatattatt aagagatttg
    33721 ataatcttga tggtgaagta gatagaaaaa gaacagagca aagctttttt gttgatgtgg
    33781 aagagataag agagaatggg tatgatttat ctattaataa gtataaagag gtagtgtatg
    33841 aggaagtagt ttatgatgct cctagtgtga ttataggtag agttagggag ttagagaagg
    33901 aaatcgtaga tggattagag gaattagaaa gaatgcttga ggtgtagagt atggaatata
    33961 gattgaatga agtttttgaa tttattagaa atggggctag tattaaacaa tttgatacaa
    34021 aatttggata tccaattaca agaatagaaa ctatagcaaa ctcttatatt gatactaata
    34081 aaatgggata tgctgacata aaagagttag gaaaattaga agactattta ttgaaaaatg
    34141 gagatatact aatgtcacat attaatagtg aaaaacattt agggaaaagt gcaatatatg
    34201 aagatataaa taaaaaaatt atacatggaa tgaatctttt atgtttacga ccagataaag
    34261 aagtttgtta tccaaagtat ttaaattatt tttttaatag taatgaattt agaagacagt
    34321 taccaaggat aataaaaaaa tcagttaatc aagcaagttt ttcagttaat gatttaaaaa
    34381 atttaaaagt agatttacct gaaatggaag ctcaaaaaga tatatcaaaa gttttagata
    34441 aagctcaaga gttaatagat aaaagaaaag agcaaataga agcattggat gaattagtta
    34501 aatcaagatt tattgaaatg tttggaaatt ttaaggaaca aaagtttgta ttattgaaag
    34561 attgtacaaa ttttattgat tatcgtggta aaactccaat tctttcagag aatggaatta
    34621 gaataataaa tgcaaaatct gtagggaatg gtgtatttaa atatattaat gaatatatat
    34681 ctgaaaacac ctataattct tggatgaaaa gaggttttcc agtagctgga gatgtacttt
    34741 ttgttacaga aggtcataca tttggaaatg tatgtaggat accaaaggat ttaaaaaagt
    34801 ttgctatggg tcaaaggatt ataacaattc agggagataa aaaaatttta aataatgctt
    34861 ttttagcaca atatatgcaa acaaaagctt ttagggtaga aatagataaa tataaaactg
    34921 gtagctctgc acaaggaatt agaagcaaag aacttcaaaa aatattaata ccagtaccat
    34981 caatagaact tcaaaataag tttgaagagt ttgttaatca agtagacaaa ttgaaagtag
    35041 gaataaaaac aagtctaaaa gaactagaag acaactttaa ctcattaatg caaaaagctt
    35101 ttaaaggaga gttattcaac taaaatacaa ttattaaaat gggggaataa aatctatgaa
    35161 tcaaatagag ctagatttat accagaacat ggtaaatatc tataaagaag ctgatttaca
    35221 atgtaactat aagccaacaa gatttttaca actgataaat aacaaaggag ccttattagc
    35281 tgctaaagaa ttaatcaata aagaaggagc tactgaagga tttacaaggc tatgggagtg
    35341 taagagatta gatttatcat tagaagtatt agtattaaaa gaaaagtata aagagttgtt
    35401 tactgatgaa gaaagagaaa tatgtataaa tagattaaaa gattatggat ataaattcaa
    35461 ttaagcaata ttacctaata aacaagaggt gattaaatga gaaatatacc aaatgagatt
    35521 aaaaactcaa taataaatgt atttccaaaa gaaaatactc tagcaatcat aatatttggt
    35581 agttatggaa cagataaaca tacatcagaa agtgatatag acatagcttg gattcccatc
    35641 aaaaaagtac caattacaga acttgcaact aagacagaag ctctaagaaa tatattagac
    35701 attgatgtag acctaaaaat tgttacagat aattatacaa tagcacttaa aaaagctatt
    35761 ttagaaggtg aagttattta tgaatctaaa gattttaagg attatataaa taaattctat
    35821 tttgaaaaca gtgatgtaat agatatctta gattggaggg acatgtatgg taattagaaa
    35881 caaattatct tggaactcaa tagaagaagt aaatagggat tttggttctt gcttgtatga
    35941 cctgcaaaac ttcaaaatct tatatgataa ggaagaaatg cctgagttat gggctagata
    36001 tataaaagaa ggttttaaaa gctatatggt atcatttcta gaattaacaa aagcaatgtt
    36061 gtattataaa tcaattgatt taaatataaa atctaaaaac ttttatgacc accttttaac
    36121 ttgtgagtat cacaatttat tacctaaaaa ttcttctata gttatagaaa cacttagaaa
    36181 attgagaaat gatgattctc atggatatga tataccacag tttgaagata tgtacgaatt
    36241 gtttacagaa aatgaagaag tatttgttag tataagaaac agctgtaaga aatagttttt
    36301 agagagattt attttaacta agggacaaaa gggggagaaa agccttgggt aattttgatt
    36361 ttttacaaaa gaagaaaaat ttcaatagtt ttaatactgc ttgcttagaa gcagaaaaaa
    36421 gtatactagt tagcccatct acttgtgcaa ctataacaag aagagcttta gagcttgcag
    36481 taaaatggct atatgctaat gatagtgatt tagtattgcc ttatcaagat aatcttagta
    36541 cgttgattca taataatagt tttatagaac ttatagacta tgatatgtta cctttattaa
    36601 aatatatagt taaattaggt aatttatctg tacatactag tgcgaacata gagagaggag
    36661 aagcaatact ttcacttaac aatttgcatc aatttgttag ttggatagac tattgttatt
    36721 cagatgaata tacagcagaa gactttaatg aagatttatt acttcatggg gaagagaaaa
    36781 ggacgagacc agacgaactt aaaaatcttt atgagagatt aagttcaaaa gataaaaagc
    36841 ttgaagaagt aataaaagaa aatgaggaat taagaaagtc actaactgaa aagagaaaag
    36901 ttaatactgt aaactatgat ttcaagatag acgaagtaag cgaatttgag acaagaaaaa
    36961 gatatataaa tatagaatta aagcttgcag gttgggaatt tggaaaagat ataggggagg
    37021 aaatagaagt acaaggtatg cctaatgaaa gtggtgtagg atatgtagat tatgttcttt
    37081 atggtgaaaa tggtaagcca ttagcagtag ttgaggcgaa aagaacgagc aaagacccaa
    37141 aaattggtca acaacaagct aaattatatg cagattgttt ggaaaagaga tatagtcaaa
    37201 gaccagtaat atttttgact aatggttttg atatgtatat ttgggatgac tattcagata
    37261 gaaaagttta tggattttat aaaaaatcag aattacaact tatgatagat agaagaaaga
    37321 gtaagaagtc tttaagttct gtaacaataa atgatgaaat atccaataga tattatcaaa
    37381 aagaagctat tagggctgta tgtgaggctc ttgaaaataa gcaacgcaaa actcttttgg
    37441 tatgtgctac tggtacagga aaaacaagaa ttgctatatc tatagttgat gtactttcaa
    37501 gacataattg gattaaaaat atattatttc tagcagatag aaaagcattg gtaaagcaag
    37561 ctaaaaaaag ttttactaag ctattaccta acttggcgtt atgtaattta ttggatagca
    37621 aagacagccc agaagatgct aggatgatat tttctacata tccaacaatg atgaatgcaa
    37681 ttgatgatac gaagtctaag gatggaaaga gattgtttac acctggtcat tttgatttaa
    37741 ttataataga tgaatcgcat agaagtattt ataaaaagta taagtcaatc tttgattatt
    37801 ttgattctta cttaattggt cttactgcta caccaaagga tgagatagat aaaaatactt
    37861 attcagtttt tgacatggaa aatggagtac ctacatatgc ttatgagtat aataaggcag
    37921 tcgaggatgg atatttagta gattatacta gtattgagtt taagactaag attatggaag
    37981 atggaattaa gtatgatgag ctttctgatg aagagaagga agaatatgaa aatacattta
    38041 atgatgatga gagtattgga gacgaaatag ggaataatgc agtaaatgag tggttattta
    38101 attctgacac aatagattta gttttaaata agctaatgac agaggggctg aaaatagaag
    38161 gagaagaaaa gattggtaag actataattt ttgcaaagaa tacaaagcat gctagggcaa
    38221 tagttgaaag atttaataaa ctgtatccaa agtatggtgg gaactttgta aaagcagttg
    38281 actatagtat aaattatgta gattctataa tagatgattt ttctgataag aataaattac
    38341 ctcaaatagc agtttcagta gatatgcttg atacaggaat tgacatacct gaaatactaa
    38401 atcttgtttt tttcaagaaa gtgagaagta agactaagtt ttggcaaatg ataggtagag
    38461 gaactagatt atgtccagat ttattaggtg ttgatatgga taaggaaaga ttcttaatat
    38521 ttgatttttg taataatttt gaatttttta aatttaatcc taaaggattt gagggaaata
    38581 aagcagaaac acttactgaa aaattattta atataaaggt aagtatggtt aaagaacttc
    38641 aagatattaa atatatagaa ggtgagtatg cagaacttag aaaagaattg ttagaagaac
    38701 ttataacttc tgtaaaagct ttaaatgagg atagttatat agttaggatg aatttaagtt
    38761 atgtacataa gtataaaaat gaaaatgttt ggagcaatat tggagcagta gcacaaaatg
    38821 agataagaga atatatatca ccactcataa cttcatatag tgatgatgaa ttggctaaaa
    38881 gatttgatgt agttatgtat aatatacaac ttgcatactt acaaaataat aatgcaagta
    38941 aaggtataag gcatgtaatg gctacagcag aaaaactttc aaaacttggg actataccac
    39001 agattcagca acaaaaatat actattgaaa aagcaatgac agaggatttt tgggaagatt
    39061 cagatatatt tgaggtagaa gaagtacgta tatcattaag agagcttatt aagtatcttg
    39121 aaaagtctag tcaaaagata tattatacaa gttttgaaga tatgattgta gcagaagata
    39181 gaaatgattc tgtatataat gcaaacaatc ttaagaatta taaaaagaag gtagagtatt
    39241 atttaaatag ccataaagat gaattagcaa tatttaaatt aagaaataat aaaaagataa
    39301 caaagcaaga tgttgaaacg ttggaggaaa tactcttaaa acagttaggt aattgtgatg
    39361 attataagaa ggagtttgga gatactccag taagtcaatt agtgagaaaa ctagtgggat
    39421 tggatagaga agctgcaaat gaagcttttt cagagttttt aaataataag agctttaata
    39481 ctaagcaaat acattttgtt aaacttatag tcgattatgt agtcaagaat ggatttatag
    39541 aggataacaa agtgttgatg gaagacccat ttagaactgt tggaagtata attgatttat
    39601 ttgaaaatca tattgaagag agaaataaat tgattaaaac tataaataaa attaaagaaa
    39661 atgcatcaga gataggctaa tgatgttatt tagtgaaaaa ttattgttaa tgatgtagag
    39721 aataaattta ttagtaggta gtctaatatt gggttgaaat agataactag atatggataa
    39781 tgtaaatata caaagagagg tattgaataa ttatgcaata tctcttttta attgataaat
    39841 aactatgaat tactcatgaa gaagattaaa taataatttt taaaattaag tatttgtata
    39901 ttttatagaa taatcatgta tttactttaa taaaataaaa acaggtataa aaaggtatat
    39961 aataaggctt tcatatacat ttatgagtca aaattttgat ataatatata gatacatgga
    40021 gttatgaatg gaatttattt aacttatagg gggagttaat atgaaagcag gagaaattga
    40081 atttcttagt tatttagagg ggtcaaacaa aagttttgta gtacctgtat atcaaagaaa
    40141 ttataactgg aagaaagaac aatgtaaaag attgtttgat gatttagagg atataataga
    40201 ttctggattt agaactcatt tcttagggtc agttgtatct atatatagta tgggaaaaga
    40261 atatttaata atagatggac aacaaagagt gactactgtg tcaatattat tgattgccat
    40321 gtgtcaaata ataaaagagt ttggggaatc aaaaggaatt attacaacag aagaacagat
    40381 taaggagtgt tatcttataa acaagcactc aagagataaa gatggaaaaa taaagttaaa
    40441 accaatagca gaagatagaa aagcgtacta tgaactattt gaagaagaaa tgaatacaga
    40501 tagaaattct aatatagttt caaattattt atatttttat aatagaataa aagaagataa
    40561 tatatcaata gatgatttgt ttgatgctat acagaaatta actatagtag aaatagagtt
    40621 aaaaaatggt gaagatgacc ctcaattaat ctttgaaagt ctaaattcaa ctggcttaga
    40681 tttaagtgaa gctgatagag taagaaactt tgtgttaatg ggtagaagct ctaagttaca
    40741 agaggaattt tataataatt attggtataa aattgaaaaa aatacaagta gcaacgtaag
    40801 tagttttata agagactatt tgactataaa ggagagaagt ataccgaata aaaataaagt
    40861 atattttagt tttaaaaggt atgtatcaga taatggtata gatatagaaa ttttattaaa
    40921 agatttactt aaattttcta actattatag agagatatct tattcatgta ctgatgataa
    40981 tgatgtaaac aaagttttga aaaggataaa caaacttgaa atatttgtat catatccgtt
    41041 tatattagaa ttatttgatg attataatga aggtataata aataaaaaag tacttgtaga
    41101 atcccttaat ttagtagaat gttttatatt aagaagaata gtatgtgatg tacctcctaa
    41161 tgcactgaat aaagtattta tgaacttagg aagggaaatt aaaaaattta gtgattataa
    41221 agaaaactat tctaatatat taagatatat ttttataaat aagaaatcat ctcaaagatt
    41281 tcctactgac agtgaatttt caaatgcttt tgaaactaaa gatatatata atactaagtc
    41341 aaagaataag ttatatatac ttgagaggtt agagaattat aataataaag aaattgttga
    41401 tttagaaaat ttaatcaata ataatatgct aaatatagag catataatgc cacagacatt
    41461 aacaaataaa tggaaggaat cactaggaga taactataag gagatacatg ataaatattt
    41521 acatacaatt ggaaatttaa ctttgacagg atataattca aaactaagta ataaaacatt
    41581 tgaagaaaag aaagaaatgg aagatggatt taaagatagt agactttatc taaataaata
    41641 tatttcatct atagataaat ggaatgaaga agaaataaaa aatagaacta agatattact
    41701 agatagagct gtagaaattt ggaaatatcc aagtgttaca tatgaacctc caagtaacag
    41761 tgaaaatata tttacgttag aggatgatga tgttaatttt acaaatgaaa gtatagtatc
    41821 atttagtata ttaggagagt catataaggc taaaaattgg acagaatttt atgaacaagt
    41881 tgctcttact ttatatgatt tggattctgt aaggtttaac agtattgtag acaaaactta
    41941 taaaagagat tttatagata atattaagtc tagagatgca tataaattaa gaaaaggtat
    42001 taaaatatca gaaaatatat atttagaaac aaatttaaat acagaggcta aattaaatat
    42061 aattagattt ttacttaatg agtttgatat agagttaaat gaagtaatat tttccataaa
    42121 ataatgaaat atatgcattt tttgcgtaat agaatattaa ttatattaaa aataaaatag
    42181 cacctcttga tatagattta tagctcaaat ttatatcaag aggttacgtt
//
LOCUS       DBGJFF010000022        41074 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.022, whole genome shotgun sequence.
ACCESSION   DBGJFF010000022 DBGJFF010000000
VERSION     DBGJFF010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 41074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41074
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.022"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            855..2525
                     /gene="pdcB"
                     /locus_tag="ACOIAF_001191"
     CDS             855..2525
                     /gene="pdcB"
                     /locus_tag="ACOIAF_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase PdcB"
                     /protein_id="HGZ2668210.1"
                     /translation="MREESNSLSLDHIEIFFELLSQSTEDYIFFWDINRNKFKISSAI
                     FDEFNLSKEIESDLVNCWSKIVYPDDVQIWKDDIQELLHGKKGEHNLEYRLINKYKEI
                     VWISCRGKVYVSDDPKTIFLVGRIKNIGEKNKFDSITGTWNREQFEHRMNYLIKEKIY
                     KNGAMFIMDIDNFKNINEKYGHSYGDKVLRAIATEVLEYLPKDVRLYRLDGDEFAFFY
                     PMCTKETIEKIYEKIQMYTNTQHEIESSKYYCTVTAGVAMYPEDGDNYLDLFKHADIA
                     LDIAKISGKNRIKFFSQELYENKLKVISMQQKLRECVENNFNDFELFFQPQVNAVTKE
                     VIGAEVLLRWHSSTYGEVSPVEFIPILEQSNLIIPVGKWIIKEAVKQCKEWHKINPDF
                     KISVNVSYIQLKEDFFRDFIVECLVEYQLRPEFLILELTENCWIPDINLLNDKFISLK
                     GIGVYIAIDDFGTGYSSLNYLKELSVNIIKIERSFVKNITYNSYEYTFLEYIIKLAHI
                     INLKVCVEGIESYEEYDIVKSLGVDIIQGFLFGRPVSASEFYKFQLSK"
     gene            complement(2647..3381)
                     /gene="pflA"
                     /locus_tag="ACOIAF_001192"
     CDS             complement(2647..3381)
                     /gene="pflA"
                     /locus_tag="ACOIAF_001192"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HGZ2668211.1"
                     /translation="MIKGKIHSIETFGTVDGPGIRYILFFQGCPLRCKYCHNRDTWDI
                     KSGKEYTVDEIITDALKYTSFMKFSGGGITASGGESTLQPEFLSELFKKAKENDIHTC
                     LDTSGFVDIETIDPVLDNTDLVLLDLKHMVEEKSIDLTGVGMDKALKLARHLESRNIP
                     VWIRHVLVPGITDDIDNLEKLGQFVATLKNVERFELLPYHSMGIHKWENLGIDYELKD
                     VPDATKEDIQKASEIISKFGVKVYNS"
     gene            complement(3541..5772)
                     /gene="pflB"
                     /locus_tag="ACOIAF_001193"
     CDS             complement(3541..5772)
                     /gene="pflB"
                     /locus_tag="ACOIAF_001193"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HGZ2668212.1"
                     /translation="MNAWQGFKTGRWTKEINVREFIQLNYSPYEGNDSFLAGATENTK
                     KLWDEAMVLFKKERENGGTLDVDTKTVSGIAAYAPGYLDKELETIVGLQTDAPLKRAV
                     MPYGGIKMVENSCEAFGYELDPEIKDIFTKYRKTHNQGVFDVYTPEMRAARKSGIITG
                     LPDAYGRGRIIGDYRRVALYGVDALIEDKNEQKKSLEVSCMDEEVIRLREEITEQISA
                     LNELKKMAESYGFDISKPATNSKEAVQWLYFGYLGAVKDQNGAAMSLGRTSTFLDIYF
                     ERDLKAGIVTEEELQEYMDHFVMKLRMVKFLRTPDYNNLFSGDPTWVTECIGGMGIDG
                     RTLVTKNSFRMLNTLYTLGPSPEPNLTVLWSTKLPQGFKDFCSKVSIDTSSVQYENDD
                     LMRAYWGDDYGIACCVSAMRIGKQMQFFGARVNLAKTLLYAINGGVDEKSGVQVGPRF
                     EPITSEYLDYDEVMSKFEPFTDWLATLYVNTLNVIHYMHDKYSYEALEMALHDRDIFR
                     TMACGMAGLSVCADSLSAIKHAKVKTIRNEQGIAVDFEIEGDYPKYGNNDDRVDSIAV
                     ELVESFMNKIRKNKTYRNSYPTQSILTITSNVVYGKKTGNTPDGRRAGAPFAPGANPM
                     HGRDTNGALASLSSVAKLPYEHAQDGISNTFSIVPAALGKDMTERINNLSAMMDGYFA
                     QNAHHLNVNVFDRATLEDAMEHPEEYPQLTIRVSGYAVNFIKLTKEQQLDVINRTFHG
                     KMA"
     gene            6131..7126
                     /locus_tag="ACOIAF_001194"
     CDS             6131..7126
                     /locus_tag="ACOIAF_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HGZ2668213.1"
                     /translation="MFITVVLFLVGFLLITKGADIFINCTVEIGKKTNISEIILGATI
                     VSFATTLPEFTVSLLASIDGHTTMSLGNAVGSIICNTGLALGLVVFISPFNVDKKMFF
                     SKSLLLIVSVIVLILLSLDGVITRGDSLLLIIILIFYMVNNYRSVVGKSDTKNRNIKN
                     NTIKDISTKKGKNYRGFSVLEIVKILLLFATGLIMMIIGSQILIESGVIIASFLNIPQ
                     GIVSLTIIALGTSLPEIVSSITAIRKNHHEISVGNILGANILNIVSVIAVSAIPNNIP
                     ILSQNRQLDIPFMILLLLIVIIPTLKSNKLSRIQGILMLFTYFLYISILYFMYII"
     gene            7217..8830
                     /locus_tag="ACOIAF_001195"
     CDS             7217..8830
                     /locus_tag="ACOIAF_001195"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HGZ2668214.1"
                     /translation="MNITKFEDLPISEGIKKAIAEMGFEEPSPIQAQSIPAILSGKDV
                     IGQAQTGTGKTAAFSIPILETIDPNNRSLQAVVLCPTRELAIQVSTEIRKLAKYSHGI
                     KTLPIYGGQPIDRQIKSLKSGVQVVIGTPGRTIDHINRKTLKMDNVKMIILDEADEML
                     DMGFREDIEMILSKIPEERQTTFFSATMPRGILELTKRYQKDPEHIKVVRKELTVSNT
                     KQYYIETRSSNKLEVLCRLVDVYDPKLSVVFCNTKRKADELVGDLQARGYFADALHGD
                     LKQTQRDIVMDKFRNGTIDILVATDVAARGIDVDDVECVFNYDLPQDEEYYVHRIGRT
                     GRAGREGMSFTFVFGKEMRKMKDIERYTKSKLIKHNIPTITDVEEKKVGTFFAQVKQT
                     IEEGHLTKQLQWLEGFCNDEDYAMVDIAAALVKLSLGEEMKEEIIEEKPRRERGDRKG
                     GTGAKDGMIRLFINIGRNQRVQAKDIVGAIAGEVGIPGKVVGTIDIYDKYTFVEIPKK
                     DAKTVIEKMKDIKIKGNKINIEKANKKKK"
     gene            9161..11515
                     /locus_tag="ACOIAF_001196"
     CDS             9161..11515
                     /locus_tag="ACOIAF_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HGZ2668215.1"
                     /translation="MDIIKKLSHGCTLDCHDCCKFNVYTKGNNVVKIEGDKNHPYTKG
                     FICKKGMAHLDRLNHKDRIKTPMLKVDGVWKEISFDKAIEIMAEKLTYYKEKYTSKSV
                     MHYDQYGSGSVLKYIGDIFFNFYGGVSRHKGGPCWSAGMHAQKYDFGVAKSHAIEDML
                     NSKSIFVWGKNPAYTTIHTMQIIKKAKEKGIKIVVIDPIYTKTAQIADKYVQVNPGTD
                     GALAIAMAKIIVEDKLYDEEYINSYVIGFEEYKKYLSSLELSFLIDECGVKENDIREL
                     VDLYTNKYSSINVGYGLQKYKNGGNTIRAIDALGAITGQIGFSGGGVNYANKVYPSVI
                     NSDPYNSQSYGEDREFYVSNISKFIEESLKNTSNKVNYASDELDMALHKVNYISDELD
                     TTSNKVNYVSDELDMALHKVNCVSDELDMALHKVNCVSDELDMALHKVNYVSDELDMT
                     SNKVNYVLDELDITSNKTDYISNELYNLSNKSIKDNIPIKMAVITKSNMLNQLPDLVE
                     LERVFSKIEFKVCFDMFITDTATLCDMFIPCTNTLESEDIIYSSMTNPYITYNERAVK
                     PAHKLMDEYYFFMELAKKMGLNDYPFVEKRTYLEKVIEPLKRFDKNLDIEKLKNNYFT
                     IHNPVAWEDKKFETPSGKYELYSESIKNLGISPTPVYISNKYKEIEDKNISFRLLTNH
                     HADTLFSQHFMDKKSIAQAYINRRMAKKVGIEDKDIVILRSKKAKINVQINIDDGVGN
                     YIVKMYVGWWKKHGNPNSLTDTGISDFGGQVTYNESMVEIIRQN"
     gene            11603..11938
                     /gene="spoIIAA"
                     /locus_tag="ACOIAF_001197"
     CDS             11603..11938
                     /gene="spoIIAA"
                     /locus_tag="ACOIAF_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045152 - antisigma factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor antagonist"
                     /protein_id="HGZ2668216.1"
                     /translation="MVNYSLEHKNLYIEFMCSELDHHVANEIREEIDNLLSVNQVKNV
                     VFNFENINFMDSSGIGVIIGRYKKISNEGGRVSVINISSRVKKIFDLSGLNKIIGIYD
                     TYEEALSSL"
     gene            11952..12380
                     /gene="spoIIAB"
                     /locus_tag="ACOIAF_001198"
     CDS             11952..12380
                     /gene="spoIIAB"
                     /locus_tag="ACOIAF_001198"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA];
                     GO_process: GO:0042174 - negative regulation of
                     sporulation resulting in formation of a cellular spore
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor"
                     /protein_id="HGZ2668217.1"
                     /translation="MNNIMEVKFSAISENESLARVIVASFAAKLDPTVDELVDIKTAV
                     SEAVTNAIIHGYEEDSSKFVFLRCEIEGNTIKIIVEDEGYGIENVEKAMEPLYTSKPE
                     LDRSGMGFTVMKSFMDDVEVSSVKDNGTRIEMTKKINVPK"
     gene            12403..13167
                     /gene="sigF"
                     /locus_tag="ACOIAF_001199"
     CDS             12403..13167
                     /gene="sigF"
                     /locus_tag="ACOIAF_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigF"
                     /protein_id="HGZ2668218.1"
                     /translation="MEVTVAREEKKPLLSHEETLELIEKVQNGDEEAKEILISSNLGL
                     VRSVVSRFLNIGYDREDLFQLGSIGLIKSIYKFDPKFNVKFSTYAVPMILGEIKRYLR
                     DDGMIKVSRSLKQIAVKAKMESEALTKKLGREPSIEELAKAIDVEKEDLVMAMEANFN
                     VEYLQGVIHEEEGSPICLIDKISMKGESEEEKVVDNILLKDILGRLDKRERQIIVLRY
                     FEDMTQSEIGEMLNISQVQVSRIEKKVLSKLKEYIS"
     gene            13447..13884
                     /gene="spoVAC"
                     /locus_tag="ACOIAF_001200"
     CDS             13447..13884
                     /gene="spoVAC"
                     /locus_tag="ACOIAF_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AC"
                     /protein_id="HGZ2668219.1"
                     /translation="MDKNYKKYVDQISPKPTYLKNYTLAFIVGGIICMIGQAINDLYM
                     KVGGLDKLGASSATSITLIFIGAFLTGLGVYDLIGKRAGAGSIIPITGFANSIVSPAM
                     EYKREGYVLGVGANLFKIAGPVLVYGIGSSILCGIIYYIFKMF"
     gene            14051..15064
                     /gene="spoVAD"
                     /locus_tag="ACOIAF_001201"
     CDS             14051..15064
                     /gene="spoVAD"
                     /locus_tag="ACOIAF_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HGZ2668220.1"
                     /translation="MKNKRIGKRTVKLENKPTIISTGTIVGPKEGEGPLKDYFDMIMT
                     DDLYGEKTWELAESKMVETASQQAIQKAGKKLSDVNYMLGGDLINQIVPASFAARELA
                     IPFLGIYGACSTMAEGLCIGSMLIDGGFADLVLSGTSSHYCTAERQFRFPLELGNQKP
                     MTAQWTVTGAGSVLLAPNGDGPKVKYVTVGKVIDEGIDDGNNMGPAMAPAAIDTIYSY
                     FEDTNDDPNSFDIIATGDLGTLGKQIAEDFLKEKGVDISKVYTDCGIEMFNLKEQDVH
                     CGGSGCGCSATVFAGYIYDKLRKKEFNKVMLVSTGALLSPTSTLQKQTIPCVAHAVVI
                     VNE"
     gene            15078..15440
                     /gene="spoVAE"
                     /locus_tag="ACOIAF_001202"
     CDS             15078..15440
                     /gene="spoVAE"
                     /locus_tag="ACOIAF_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AE"
                     /protein_id="HGZ2668221.1"
                     /translation="MLMDYVRVFIVGGIICLIAQVMMDYFKMQTPYVMVTYVTTGVVL
                     TFLGLYEPLVKFAAAGATVPIIGFGYSLATGVIKSIQSDGFLGIFLGGTTATAGGIAA
                     AIFFGYIMSVVFTPKAKP"
     gene            15623..16639
                     /locus_tag="ACOIAF_001203"
     CDS             15623..16639
                     /locus_tag="ACOIAF_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGZ2668222.1"
                     /translation="MVTIKDIARNLGISYSTVSRCLNNNPNVSEKTKKKVVEEANRLG
                     FHFNVNARNLAKKETNRIGVIFSNNFNHQDTRKFFSDIMDSSINSIETNKYDFIIQPN
                     NNISGDSNVYKMVNGQMVDGLVIVSQSIKKEEYDFLKDNNFPHVFIYFKPSFIGEVDN
                     FFWDDNVYGGYIATKHLIDHGHKDIITVTSDDKSSKMHEDRTKGYLNAMEEANLKTEV
                     IKSKMDFESQVEFLEKYIDKIKKASAIFVQQDVPAISIIQELKTTYGINVPEDISIIG
                     YNNIELISYFRSHLSTIDDPREQVIKNGVDSLVNIINKKSTEHIPRKLYPRLIIRNSV
                     KRIK"
     gene            17014..17856
                     /locus_tag="ACOIAF_001204"
     CDS             17014..17856
                     /locus_tag="ACOIAF_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HGZ2668223.1"
                     /translation="MKEKAGIRNAIIAAFIITILAELIGPISFKVMGINVTLLSILWA
                     IFIGMAVSPHLLGRVIPALKKFIGDNEINVSPYLLSLTLYPLGIMFGINAGPKVGILL
                     QAGPALLLQEFGHMGTMLIALPVGVALGLGRSALGGTFSLCRDTALGIIGDKYGLNSP
                     EGIGTLGTYISGSIFGTLLFSFLAPVGVLLGFHPYALAMASGMGSGSMMGAATASLMN
                     TVPQMSEQILAYSATSGLITAVTGIYIELFLSLPVANYYYSKVAPVIERFRGRKTRRT
                     SNKL"
     gene            17871..18335
                     /locus_tag="ACOIAF_001205"
     CDS             17871..18335
                     /locus_tag="ACOIAF_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668224.1"
                     /translation="MEKTFKTIMEQIKIVLFIYIIICFGQMIALKTQAQDLLIGSVSG
                     FLLVIIAIIIKNFVKKPNLPGFAWATLVAFVLSLPISPVGDIIATNVGKINFMSTVTP
                     LLAFAGISVGNQLDILKTLSWKLVVISFIVMTSTYFGSAFISQTVLRLNGML"
     gene            18349..19494
                     /locus_tag="ACOIAF_001206"
     CDS             18349..19494
                     /locus_tag="ACOIAF_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HGZ2668225.1"
                     /translation="MNELKAKIKEEIKLLSEEKKTSFEKVSDYLFSNPELAFEEYKSQ
                     KALCDLLEENEFNVTKGVGGLETSFEAVYSNGTNGKTVAFLAEYDALPGMGHACGHNI
                     IGTSSVGAGIILKEIMKKHNIEGTVKVFGTPAEERVGGKITMIKEGVFNNVDAALILH
                     PSDASMPDDISFAQVNLKFDFTGKASHAAAFPWEGKSALSGVIALFNSVNSMRLHLKD
                     YARVHGIITDGGSIHNIIPEKSTAIFNVRALSIEYLNEICEMLKNCAKGAAISTGTNV
                     EIVQLDEIYKEIKNDSELVNIVRQNFEVLGEDYVERDLSQGIGSTDTGNLTHEIPAIQ
                     AYIKLKENTATHTDEFAVAAGGEEGRVALIKAIKVLAMCGVDILYSK"
     gene            19538..20473
                     /locus_tag="ACOIAF_001207"
     CDS             19538..20473
                     /locus_tag="ACOIAF_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1177 domain-containing protein"
                     /protein_id="HGZ2668226.1"
                     /translation="MILKQVIEVYDILDKANANGEEVKTYLQGYGEVDVTVKELSSSK
                     GSTDLVKLTIPGKNGKLKGGDAPTLGILGRLGGIGARPEVIGFVSDGDGALVAIAVAA
                     KLLDMQRKGDILDGDVVISTHICPDAPTKEHYPTPFMDSPIDMMTMNENEVDSSCDAI
                     LSIDTTKGNRVINTRGFAISPTVKEGYILKTSDNLLDIMQTVTGKSPFVFPLSIQDIT
                     PYGNNLYHLNSILQPAVATDAPIVGVAITTEMPVAGCATGATNFSDLDSAGRFAIEVA
                     KLFGRNKCDFYDKEEWEMLIKRYGKLNRFQTFGIQ"
     gene            complement(20748..23441)
                     /locus_tag="ACOIAF_001208"
     CDS             complement(20748..23441)
                     /locus_tag="ACOIAF_001208"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HGZ2668227.1"
                     /translation="MNNDNDKNGNKIRRKKVSSSGNTNKPINRTSANKTRSTKNKKKS
                     KKSDKFKKLRVFGIVFLVLLVVGTAGTAGLVFASLRDVTPVTEAVLDKQTNQTTTIKY
                     ANGKTLSTAPSVNKKTPVPLDKISPYLQHAVIAIEDERFYEHKGVDIKGLFRSVLKTL
                     TGTKQGGSTIPMQVSKMLLTTEQQTIPRKIKDIYYAHEMSKTVSKDKILETYLNNFFV
                     GRGLAGAEAGARGYFDKSAADLTLAESALLAGSTKNPSRFSAYKTSKLEGNETKEDLE
                     NKLLFFVNTTDDDLDDPTQVDFDMIEKIKSWELISNDTYRQLKAGTLVVRKAVSNPEA
                     KKRQEIVLKKMLELKYIKQSEYDEAIKAKIEIKLPQSSDKVSSSVEDLIESEVINALM
                     EQGHTNDEAQNLFYNGGLIVNTTIDPKMQDALEEEFDRNSNFPGHMVGPDGVSQPQAA
                     MVILDYKNGEIRALAGGRNISGRKTLNRATNPHQPGSSIKPLAIYTPAIDTLKITQAT
                     ALSDSRGGYKFEENNKWNPRTTTAGHGSMSLRKALAKSSNTIAVKTAEMLGDSYDECV
                     DIMMDYLKNFGITTLKNNHSGSSEASDRKFPSLTLGGMANGITPLQMAAAYGTLANHG
                     IYVEPSIFTTITTFDGQLLVKNAPEEHKVVDPEVAYVVTDMLESVITEGTGGVATLPK
                     GMPVAGKTGTTNSAYDAWFVGYTPYYVGATYIGDDAGRKDDSGNTIKRREVPHGSTST
                     AKLWEKIMEKIHANLTVTEFEVPKNVYFTKINLEDGGKQSSGSKAAFIEGTAPTKVSS
                     QPSSEDTKKPDNNQPEENNNNNNNTGDNGGGSTPPDNGGNNGGGSTTPPDNGGNNGGG
                     STTPPDNGGNNGGGSTTPPDGGGNNGGGSTTPPDGGGTPAT"
     gene            23655..24353
                     /gene="yunB"
                     /locus_tag="ACOIAF_001209"
     CDS             23655..24353
                     /gene="yunB"
                     /locus_tag="ACOIAF_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YunB"
                     /protein_id="HGZ2668228.1"
                     /translation="MNRIVDRKTKNEFRKIIAIFLVILFLSVFIGSFIYIDKTLRPTI
                     TVLAETKALELANRSINKAVAEMVEGKINYEDLMDIQLDNNGKITMIQANTIMMNEIA
                     SAIALEIQDELKKDKTASSYIPIGTALGSPILAKYGPKLEVSIEPIGTVSVNFKTEFE
                     SSGINQTRHRIYLEAQTQVKVVIPLITSTKQIKAQIPICETIIVGDVPESYVNIPEKN
                     LGNVLPNTGKNTNK"
     gene            complement(24565..25572)
                     /locus_tag="ACOIAF_001210"
     CDS             complement(24565..25572)
                     /locus_tag="ACOIAF_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase S55 domain-containing protein"
                     /protein_id="HGZ2668229.1"
                     /translation="MQLKNFHFKNNTLSKKTNLAKSKFTIVLTFLFLLYFFSNNLIYA
                     QNLQKNEPEYLIPIGNVLQIDAELKNIIVRNPGEASPFRLGDAIVKVNNAEVDGYSDF
                     ANTLNALPEEKQVSILLNRGGQLITMKTTKHILEKISFNNLLSGFATLTYINPDTSEF
                     GAVGHPISVGNARKIPIKTGSISTTKHLNIEKSYKGSVGCINAKRENIIGSFTENTDF
                     GIRGQINNFDISKLKKYKVASLDEVKLGKAQIILQDNFNKCKKYNIEIIDIENQKHPE
                     SKSFKIKITDKKLLAQTGGIVQGMSGTPIVQDNKIIGAVSHAIENDPAVGYGVFIKWM
                     L"
     gene            25800..26684
                     /locus_tag="ACOIAF_001211"
     CDS             25800..26684
                     /locus_tag="ACOIAF_001211"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HGZ2668230.1"
                     /translation="MKKYRLLVTCIIAMGIIIIGTSNPVSNFYKTTNLISSGNITNEK
                     ADKDENSNKNNKDKNEDKSNSKSNKDKSSEDKSSDNKNEIQKNKKLLICIDPGHQGKG
                     DSNLEPVAPGSSSKKARVSSGTEGIATKKPEYVLNLEASLVLKSILESKGYNVIMTRE
                     THDVNISNSERAILANDKKADMVVRIHADSLNNSSKTGASILIPEKDGKYTAPIYEES
                     NKCAEFIKQNMEQSGIQINGIVQRGDLTGFNWSKVPAVLVEMGFMSNYNEDQMMSNPD
                     YQRKMMQCIADGLDAYFK"
     gene            26886..28436
                     /locus_tag="ACOIAF_001212"
     CDS             26886..28436
                     /locus_tag="ACOIAF_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007788866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HGZ2668231.1"
                     /translation="MLVVENVSHGFGARTILENVSFRLRKGEHIALVGANGEGKSSFL
                     NIITKKLMPDAGNIKWSSRATVGYLDQHTVLSKGKTIREVLREAFKHMFDLEQEMIAM
                     YDKMGEASDDEMSKLLEETAEIQTILENSGFYMIDAKIQEVANGLGLGEIGLDKDVTD
                     LSGGQRTKVLLTKLLLENPTILILDEPTNYLDEEHITWLTKYLQEYENSFVLVSHDIE
                     FINNTCNVIYHMENGELNRYKGNYDEFVRLNDIKKRQEEQAYDKQVEERKRLEDFVAR
                     NKARVATRGMANSRQKQLDKMEILERPKEKIKPTFAFKDARAASKIIFETENLVLGYD
                     EALTKPLNFHLERGKKIALKGMNGIGKSTLLKTLLGIIKPFEGNVKLGDYLEVGYFEQ
                     ESSRENSNTPMDEVWSEFPGLTNFEVRQALAKCGLTNEHITSQMRVLSGGEAAKVRLC
                     KVMLKNINFLVLDEPTNHLDVEAKDELKKAIKEFKGTVLLVCHEPEFYSEIVDDVWNI
                     EDFTTKIV"
     gene            28465..29112
                     /locus_tag="ACOIAF_001213"
     CDS             28465..29112
                     /locus_tag="ACOIAF_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HGZ2668232.1"
                     /translation="MRKFLNVLFSVCISIVIIVGVINFTVGFKQLYYFDIDYLNISEL
                     SGLSKDDIKLNYDYLIDYNLNKNVSEFKLPTLKSSPQGKIHFEEVRNIFQNINKLAKL
                     LLVVSLVGIILSVKNKNIKILKTTSITLIIMPLLLTVPILLNFEKSFIIFHKLLFRND
                     YWIFNPDLDPVINILPEEFFFHSGMMILILILLVSILLFVMYKLCKSLSIKIFQC"
     gene            complement(29254..29514)
                     /locus_tag="ACOIAF_001214"
     CDS             complement(29254..29514)
                     /locus_tag="ACOIAF_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich small domain-containing protein"
                     /protein_id="HGZ2668233.1"
                     /translation="MGENYKFFNHKDCEFFPCHKTNKPEEFNCLFCYCPLYALGENCG
                     GNFKYTDKGIKDCSSCMLPHKKDNYNYIMSKFQDLVKITSKK"
     gene            29731..31014
                     /gene="hflX"
                     /locus_tag="ACOIAF_001215"
     CDS             29731..31014
                     /gene="hflX"
                     /locus_tag="ACOIAF_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HGZ2668234.1"
                     /translation="MKEIQEKALLVGLNLTTIVKKNDDIDTNESMEELKELTKAAGAE
                     VVGSLIQNKHSVDAAYYIGKGKVEEIRAYSDSLDATLVIFNDELSGAQIRNIENVVGR
                     KVIDRTTLILDIFAQRALSKEGKLQVELAQLKYRLPRLYGMGGEMSRTGAGIGTRGPG
                     EQKLEIDKRHILNKAADIRRELKEVKKNRETQRVKRLKSNIPIVALVGYTNAGKSTLL
                     NELIKTHKDYEQEKEVFVKDMLFATLDVTLRKALLPNKKEFLVVDTVGFVSKLPHDLV
                     EAFKATLEEVQYADLILHVIDATNTSYELQKSTTEGVLKELGVNDKKHILVYNKVDKL
                     ELDIYPKSQEDIVYISAKQGINMDKLLNMIEIALMENTYSVSLMLPYERGDIFSRIKD
                     KYNVENFEYGESGITLDVDLDEEDFNIYREYILEK"
     gene            31022..31699
                     /locus_tag="ACOIAF_001216"
     CDS             31022..31699
                     /locus_tag="ACOIAF_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HGZ2668235.1"
                     /translation="MDINWENIDNLEDYFITYLLYKESKTVSQISKIRNISSTEASEQ
                     LIQAKLKIKEMQKDDFEASKDILDKFLELDKIKRLDFMDSLDDEKMVYFKRKVFKRIL
                     VEKNAEDLIVLIWATGELKDDRFLKLLHQLTNHRHSDIRRITYSAIRKIESPSSREVL
                     QKGLYDKNAQTRQYCAKALSKLGDENSLKILQQLKDKNKNFEKEYVLRAYDEAIKSLE
                     NAKNLKK"
     gene            31785..32228
                     /locus_tag="ACOIAF_001217"
     CDS             31785..32228
                     /locus_tag="ACOIAF_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HGZ2668236.1"
                     /translation="MIVRRCAGGVVFYANKVLIVKNDRGEWTLPKGKILGGGLPYESA
                     VQRVKVETGIDAKMIDVAGDTMYEFFSRSRQQEVCNAIMWYVMEACNTDCVLAPEFQE
                     GGFYKVKDALEMLSHHKEQALVEVSYKKFKELKKLVSEGDDVPAN"
     gene            32533..33177
                     /locus_tag="ACOIAF_001218"
     CDS             32533..33177
                     /locus_tag="ACOIAF_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HGZ2668237.1"
                     /translation="MSNYRTLHEFGTDEITIEKSVFIGYAKPIKSEEEALEFINEIKK
                     KHKDATHNVWAYTVGKNMNIQRYSDDGEPQGTAGIPTLEVIKKEDLRDVAVVVTRYFG
                     GTKLGAGGLVRAYTKGAKLGLEAGKIIYKVMYQEVKVKIDYTQLGKVQNELMNLGYFI
                     KDTVYEDNVEIVVYSRLEDVEKLSEKMIDITSGTGKIVLGEEFYLSEQNGEILI"
     gene            complement(33263..33640)
                     /locus_tag="ACOIAF_001219"
     CDS             complement(33263..33640)
                     /locus_tag="ACOIAF_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HGZ2668238.1"
                     /translation="MLFALIGFFAGIIGGMGMGGGTILIPALVLLAGIDTKIAQSVNL
                     LSSIPMTLIALAIHIKNKNVIFKLVIPIAVFGVLGAVFGSLLANYLSSDILKKIFGIF
                     LLLVGLFEVKKGFCAKSDKKPLN"
     gene            complement(33640..34026)
                     /locus_tag="ACOIAF_001220"
     CDS             complement(33640..34026)
                     /locus_tag="ACOIAF_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HGZ2668239.1"
                     /translation="MKKTDDLKKKYFDFNLKNTIIGVFTGFINGVFGSGGGTLLVPIL
                     NDIVKVEEHKSHATALSIIIFLTTASSVLYVSKGTYDVNLTIKVAVGSILGGIIGAKL
                     LNKVTGKFLRISFGIIMIIAALRMVF"
     gene            34205..34960
                     /gene="nadE"
                     /locus_tag="ACOIAF_001221"
     CDS             34205..34960
                     /gene="nadE"
                     /locus_tag="ACOIAF_001221"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HGZ2668240.1"
                     /translation="MSNIKIQIDKTVEWLINKVNEANAKGLIVGVSGGIDSAVVANLI
                     KKAFPENSMGVIMSIKSNPQDREDALKVIEGCDIEYLDLDLIEPQSAILDMVVGNLKD
                     KHLYREEYLKMTDANLRARVRMSTIYTIANNLGYLVVGTDNAAEIHTGYFTKFGDGGV
                     DILPIANLTKGEVYEWAKELGVHEDLINKAPSAGLWEGQTDEDEMGTTYNMIDAVLEG
                     RLDEVPKRDQEIIERLHRLSEHKRKTPAQPPKF"
     gene            35043..35762
                     /locus_tag="ACOIAF_001222"
     CDS             35043..35762
                     /locus_tag="ACOIAF_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGZ2668241.1"
                     /translation="MGRIGNIINRKGKQDAQRAKIFTKHARAIAVAAKEGGADPEYNA
                     ALKTAIEKAKADNMPNDNIDRAIAKGAGAGAGEDYETIVYEGYGPGGVAVIVETLTDN
                     KNRTAGNVRYYFDKNGGNLGTSGCVSFMFDKKGQILVGLGDGVSEEELMDVALEAGAE
                     DFITEEDGYEIITTPEDFSSVRDELKAKGYEFISADVKMIPQTTTVLTEESHLKMMNK
                     LVDMLEEDDDVQDIYHNWEVE"
     gene            complement(35929..36933)
                     /locus_tag="ACOIAF_001223"
     CDS             complement(35929..36933)
                     /locus_tag="ACOIAF_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HGZ2668242.1"
                     /translation="MATIKDIAKDCGLSSATVSYVLSGKGNERRIPLHTQELILNSAD
                     KLGYKRSTPIKHNNIKIAFYWQDEGFELASTNILQAINHIISLEHLNINIIIRPYKVG
                     HLCDDEYFFANNYYDGIVLTGINADDIEYLVKNKPKIPVVVVNREIPGFSSVSIDNEE
                     TGRIAALHAIQNGKNDIAVVFNPTSFTCMTLRGKSFLKTCKEHGIHLSDSIYYCSNKI
                     DSGYDIAQKMLAKNSLKKVIFCNHDVVALGMMNAFHESGIVIGEDIQIFAASNGPSRL
                     MARSIPPLTVIDLKMEETAERSLRMIIDTVTGRINGIQQQVIYPSIIYRQSSPAQNLL
                     "
     gene            37199..38095
                     /locus_tag="ACOIAF_001224"
     CDS             37199..38095
                     /locus_tag="ACOIAF_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HGZ2668243.1"
                     /translation="MSKVNIFEVGPRDGFQNLKEFLETDKKLKIIDGLIASGIKQLEI
                     SSFVSPKAIPQMKDAKEVATYCVEKYPDVKLYALVPNLYGAKVAWECGIRDISYVISL
                     SETHNKKNINRTHEESIDELKKIMNTYPDMNICIGMATVFGCPFEGLPKIEDAIRFAK
                     RLWENGIREINLADTIGIADPKQVRETISALVEALPECRWQVHIHDTRNMGMVNTLVA
                     IESGITTVQSTLGGLGGCPFAPGASGNTATEDLVYMLERMGYDTGVDFVKLLELSKYQ
                     KSFVNGVYSGHHVNIKSDSCNC"
     gene            38233..39495
                     /locus_tag="ACOIAF_001225"
     CDS             38233..39495
                     /locus_tag="ACOIAF_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HGZ2668244.1"
                     /translation="MDIIALLLFLGVIIIAFVRKNNAGILALAIGVIAVRIFGMDDKE
                     LYGAISNSMFTTLVGITLLFAIVNSTGALKLVAEKIVAASGKRVWLIPIAVFIAGFVV
                     AGVGPGAIPALAIIPAMAVPVAITVGYNPVMLALIGECGLMAGRMTPITPEGQIITTV
                     AEGVGIENVMPTILACQTLSTIVFSIVLFIIFKGYKLKKPINVIDSKQIEKFNIKQII
                     SLISIVVMIAFIIIFDMNVGLAAFAVSAVLIFFNIADEGECIKSIPWSTIVMVLGVGA
                     MMTIVDAAGGIDLMSNALSSLMNSKTATPIMSISAGLMSMVSSALAVVYPTMMPMCVD
                     IAKAVGGVDPLALIAAVGVGGSLAGVSPLSTGGALILAAMGSSKKDFNKEEQNKVFVQ
                     LFIMSAVGLLVIAVVSILSFNAITHLMN"
     gene            39571..40764
                     /locus_tag="ACOIAF_001226"
     CDS             39571..40764
                     /locus_tag="ACOIAF_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HGZ2668245.1"
                     /translation="MKKGALSNIKVLDLTRVLAGPYCTMMLADFGADVIKIEMPRKGD
                     DTRGFGPFVNGESLYYANINRNKKGITLNLKSEEGKNIFLEMVKHADVVVENYRPGVM
                     EKLGLGYDELAKVNNQIIYGAVSGFGCYGPYSERPGYDIIAQAMGGLMSITGQEGGEP
                     TRVGNAMGDVLGGMNLTIGILTALNARKIIGKGQRVDVSLVDSVVSSLETGIQRYFDS
                     GQPELMGNRYASAYPYDSFKASDGSFVIGCGNDKLFEKLCKKVLFREDLLIDERFKTN
                     LNRCEHYKALKPEIEKWSVKHTIKEAVDLISEAGVPAAPIYNLDCVTTDEHIAKAREM
                     IVDISHPIIGNMKVNGNPVKLMTTKAKIDMPAPSLGESNEEIYNGWLGFDSDKIKDLK
                     ENGVI"
BASE COUNT        15504 a         4955 c         7092 g        13523 t
ORIGIN      
        1 ttttataaca aatggaaact atggatgtaa taattaaaat aaaaaatatt attttatatt
       61 tataaacatt gacattctaa ttttgtagtg taatcgtatt ttttattaat tcttacaaaa
      121 tcaatgaaaa taagtatatc cattttaaag tacataaatt taaaaatcca gaaaaatata
      181 cttaaattat gtaagtaaaa gaggggttga atcagttgaa aatgtaatat tagtaaatag
      241 tacatcaatc ctatataccc tgtaagcaac tctattttca tgtactaaaa atgtacctat
      301 tttattaact gaaaagaata aattaaatgt aagtacataa gaagatagaa aaaattaaaa
      361 acaaaaaata tttatataaa taaatataaa aaatgatata atccctatta aaaaatttat
      421 agatgatgaa gaaatatcaa aatcttatat tataggaaga gacacttctt tgccaaaatc
      481 tatagaaaag caataccaaa tccaaagaga ataggcgaag aaaaaaataa aataaattca
      541 agagtaatag aaaagtttta tagaataaag aactgataac tctttatata acgaaaaata
      601 gaattttaag agaaaatgat ttaataaatg atttaacatg gtgttttagc aggtaaaaat
      661 aaatcaccag taatgattat agaaaaatta ctttatgaat ctcaaaaaac ttagttaaga
      721 gtaagagatt tgataagata actaaagttg gggaaatgtg tttgatgaga taaaaaatta
      781 gtaaaatagt ataatctaaa taaggatatt ttgttgatta aactacacta atagtaatat
      841 tgtaggggga aaagatgaga gaagaatcga actctttaag tttagaccat atagaaattt
      901 tctttgaact tttatctcaa agtacagaag attatatatt tttttgggat ataaatagga
      961 ataaatttaa aatttcttca gcaatttttg atgaatttaa cttaagtaaa gagatagaat
     1021 ctgatttggt caattgttgg agtaaaattg tttatccaga tgatgttcaa atatggaaag
     1081 atgatataca agaattacta catggaaaaa aaggtgagca caatttagaa tataggttga
     1141 ttaataaata taaagaaata gtatggattt cttgtagagg taaagtttat gtgtcggatg
     1201 acccaaaaac tatatttcta gtcgggagaa taaagaatat tggtgaaaaa aataaatttg
     1261 attctatcac aggaacgtgg aatagagaac aatttgaaca tagaatgaat tatcttataa
     1321 aagagaaaat ttataaaaat ggtgctatgt ttattatgga tattgataat tttaaaaata
     1381 taaatgaaaa atatgggcat tcttatggtg acaaagtatt gcgtgcaatt gctactgaag
     1441 tattagaata tcttccaaaa gatgtaagat tgtataggtt agatggagat gaatttgcat
     1501 ttttttaccc tatgtgtact aaagaaacta tagagaaaat atatgaaaag atacagatgt
     1561 atacaaatac tcagcatgaa attgaatcaa gtaagtacta ttgcacagtg actgcaggcg
     1621 ttgctatgta tccagaagat ggagataatt atttagattt atttaaacat gctgatattg
     1681 cattggatat agcaaaaatt agtggtaaaa atagaattaa gtttttttca caagagctct
     1741 atgaaaataa actaaaggta atttcaatgc aacaaaaact aagagagtgc gttgaaaata
     1801 attttaatga ttttgagttg ttttttcaac cccaggtaaa tgctgttaca aaagaggtaa
     1861 taggggcaga ggtactttta agatggcatt cgagtactta tggagaagta tctcctgttg
     1921 aatttatacc aatactagaa cagagtaatt taattatacc agttgggaag tggattataa
     1981 aagaagctgt aaaacagtgt aaagaatggc ataaaataaa tccagacttt aaaatatctg
     2041 taaatgtatc gtatattcag ttaaaggaag atttttttag agattttata gtagaatgtt
     2101 tagtagagta tcaattaaga cctgaatttt taattttgga attaacagag aattgttgga
     2161 taccagatat aaatttacta aatgataaat ttattagttt aaagggtata ggagtatata
     2221 ttgcaataga tgactttggt acaggatatt catctttaaa ttaccttaaa gaactgtctg
     2281 ttaatataat aaaaattgaa agaagttttg ttaagaatat tacatataat agttatgaat
     2341 atacttttct tgaatatatt attaaattag ctcatattat aaatttaaaa gtttgtgttg
     2401 agggaataga gtcttatgaa gagtatgata tagtaaaaag cttaggtgta gatattattc
     2461 agggtttttt atttggaaga cctgtaagtg cctctgaatt ttataaattt caactatcaa
     2521 agtgatgtat ataaaatgag atatatagta tataaaaata gggcaaagtg gaagttttga
     2581 agtttattaa aaatttacac tttaccctat tttgttatgt cttctataaa ggattaatat
     2641 actacactaa ctattatata ctttcactcc aaatttagag attatttcac tagctttttg
     2701 gatatcttct ttagtggcat caggaacatc ttttagttca taatcgatac ctaaattttc
     2761 ccatttgtgt attcccatag aatggtatgg tagtaattca aatctttcta catttttcaa
     2821 tgtagctaca aattgaccta gtttttctaa attatctata tcatcagtaa tgccaggaac
     2881 taaaacatgt cttatccata caggaatatt tcttgattct aagtgtctag caagttttaa
     2941 tgccttgtcc ataccaacac cagtcaaatc aatagatttt tcctcaacca tatgttttaa
     3001 atcaagtaaa accaaatcag tattatctaa aactgggtct atagtttcta tatctacaaa
     3061 tccagatgta tccaagcaag tgtgtatatc attttctttt gcttttttaa ataattcact
     3121 taaaaattct ggttgaagag tggattcacc accagaggca gttatacctc caccagaaaa
     3181 cttcataaaa gaagtatact taagagcatc cgttattatt tcatcaactg tgtattcttt
     3241 tcctgattta atatcccatg tatccctgtt atgacagtat ttgcatctaa gaggacaccc
     3301 ctgaaaaaat aatatatatc ttattcctgg tccatctaca gtaccaaacg tttctataga
     3361 atgtatcttt cccttaatca tagagttcac ctcacaatat attttaataa ccttatcaat
     3421 agttataata tttctctaaa aaaggccatc ttataccaga aataattcaa atataagata
     3481 gccttttatt tttatttttt acacagtagt aatcttagtt aaagtataaa aatttaaaga
     3541 ctaagccatt ttaccgtgga atgttctgtt tataacatct aattgttgct ctttagttaa
     3601 ttttatgaag ttaactgcat atccagatac tcttatagtt aattgtggat attcttctgg
     3661 atgttccata gcatcttcta aagtagctct atcaaataca tttacattta agtgatgagc
     3721 attttgagcg aagtatccat ccatcattgc agaaagattg tttattcttt cagtcatatc
     3781 ttttcctaaa gcagcaggta ctatagagaa agtattagat ataccatctt gagcatgttc
     3841 atatggtaat ttagcaactg aagataatga agctaaagca ccattagtat ctctaccatg
     3901 cattgggtta gcaccaggag caaatggagc accagctctt ctaccatctg gtgtattacc
     3961 agttttctta ccataaacaa catttgaagt tattgtaagt atagattgag taggataaga
     4021 atttctataa gttttattct ttcttatttt attcataaag ctttcaacta actctacagc
     4081 tatactatca actctatcat cattatttcc gtattttgga taatctcctt ctatttcaaa
     4141 atcaacagct attccttgtt catttctaat agttttaacc ttagcatgtt ttatagcaga
     4201 taatgagtct gcacaaactg ataacccagc cataccacaa gccatagttc taaatatatc
     4261 tctatcatgt aatgccattt ctaatgcttc ataagaatat ttatcatgca tataatgtat
     4321 tacatttaaa gtatttacat ataatgttgc taaccaatca gtgaatggtt caaatttact
     4381 cataacttca tcataatcta agtactcaga agttataggt tcaaatcttg gtccaacttg
     4441 aacaccagat ttctcatcta caccaccatt tatagcgtat aataaagttt tagctaagtt
     4501 aactctagct ccaaagaact gcatttgttt accaattctc attgcagata cacaacaagc
     4561 tataccatag tcatctcccc agtatgctct cattaaatca tcattttcat actgtactga
     4621 acttgtatct atagatactt tagaacagaa atctttgaat ccttgaggta atttagttga
     4681 ccatagaact gttaagtttg gttctggaga aggtcctaat gtatatagag tatttaacat
     4741 tctaaatgag tttttagtaa ctaatgttct accatctatt cccatacctc ctatacactc
     4801 agttacccaa gttgggtctc cagagaatag gttattgtaa tcaggagttc ttaggaactt
     4861 aaccattctt aatttcataa caaaatggtc catatactct tgtaattctt cttctgttac
     4921 gattccagct tttaaatctc tttcaaagta tatatctaag aaagtagatg ttctacctaa
     4981 agacattgca gcaccatttt ggtctttaac agcacctaaa taaccaaagt ataaccattg
     5041 tacagcttct tttgagttag ttgctggttt tgagatatca aatccataag attcagccat
     5101 ttttttaagt tcatttaaag ctgatatttg ctcagttatt tcttctctta atctgataac
     5161 ttcctcatcc atgcaagaaa cttctaatga ttttttttgc tcatttttat cttctattaa
     5221 agcatcaaca ccatatagag caactcttct gtagtcacca attattctac ctctaccata
     5281 agcatctgga agacctgtta ttataccaga ttttctagca gctctcattt ctggagtata
     5341 aacgtcaaaa actccttggt tgtgtgtttt tctgtattta gtaaatatat cttttatttc
     5401 tgggtctaat tcatatccaa aagcttcgca tgaattttca accatcttga taccaccata
     5461 tggcatgaca gctcttttta aaggggcatc cgtttgtaac ccaactattg tttctaattc
     5521 tttatctaaa tatcctggag catatgcagc tattccagaa acagtttttg tatcaacatc
     5581 taaagtacca ccattttctc tttctttttt aaataggacc attgcttcgt cccataattt
     5641 tttagtattt tcagtggcac cagctaagaa agagtcattt ccctcatatg gagaatagtt
     5701 taattgtata aactctctaa catttatttc tttagtccat cttcctgttt taaatccttg
     5761 ccatgcattc attaagtatt tcctccttag atttaaatga taaataatac tattaaaatt
     5821 aatttttata tacttaattt attttatata tttttttaaa aatatatttt ctaatataat
     5881 aatagcatat tattcgtatc ctgtatacat aaaaaataaa aaattgtcgc tttaaagtat
     5941 aaaacagatt atatgttaat aatgtggtga aattataccc atatataaat aaaataaaac
     6001 aaataaatag ctaattaagg agaaaatatt ttttattttt tgtttaggat aaataaattt
     6061 tattatggaa aaaagaaaaa gcatacatct tcacaaactg aatatttatt taataggggg
     6121 aggtgataga ttgtttatca cagtggtatt atttttagta ggtttcctat taattactaa
     6181 aggcgcagat atctttataa attgtactgt agagataggt aaaaaaacga atatatcaga
     6241 aattatactt ggagctacta tagttagttt tgcaacaaca ttacctgagt ttacagtgtc
     6301 tctacttgct tctattgatg gtcatactac tatgagttta ggaaatgcag ttggttctat
     6361 aatatgtaat acagggttag cattagggtt ggttgttttt ataagtccat ttaatgtaga
     6421 taaaaaaatg tttttttcta agtctttact attaatagta tccgttatag ttttaatttt
     6481 attgagttta gatggagtta taacaagagg tgattcatta ttacttataa taatattaat
     6541 cttttatatg gttaataatt atagaagtgt cgttggaaaa tctgatacta aaaatagaaa
     6601 tattaaaaat aacactatta aagacatatc tactaaaaaa gggaaaaatt ataggggttt
     6661 ttccgtatta gaaattgtaa aaatactatt actttttgct acaggattga taatgatgat
     6721 aataggttca caaatcctta tagagagtgg agtaataata gctagttttt taaatatacc
     6781 tcagggtata gtaagtttaa ctataatagc acttggaact tctcttccag agatagtttc
     6841 gtcgattact gctataagaa aaaatcatca tgaaatatca gtaggaaata ttttgggtgc
     6901 aaacatattg aatatagtat cagtaatagc tgtatcagca ataccaaata acataccaat
     6961 actatctcaa aatagacaat tggatatacc attcatgata ttattattat taatagtaat
     7021 aataccaaca cttaaatcta ataaacttag tagaatacaa ggtatattga tgttattcac
     7081 atattttctg tacatatcaa ttttgtattt catgtatatt atttaactat actgtttaaa
     7141 atattaattg cagaatatcc taaaaaagta tataatggat aggttaagat taattgataa
     7201 cggagaggaa taatatatga atataacaaa gtttgaagat ttaccaataa gtgagggaat
     7261 aaaaaaagct atagctgaaa tggggtttga agaaccatct cctatacaag cacagtctat
     7321 acctgcgatt ttgtcaggta aagacgttat aggacaagct caaactggta cgggaaaaac
     7381 agctgctttt agtatcccta tactagaaac aatagaccca aataatagaa gtttacaagc
     7441 tgtagtgctt tgtccaacaa gagaactggc aatacaagta tcaacagaaa taagaaagtt
     7501 agctaagtat tcgcacggaa taaagacatt acctatatat ggtggtcagc caatagatag
     7561 acaaataaaa tctctaaaga gtggtgtgca agttgtaata ggtacaccag gacgtacaat
     7621 agaccatatc aatcgtaaaa cattaaagat ggataatgta aaaatgatta tattagatga
     7681 agcagatgaa atgctagata tgggatttag agaagatata gaaatgattt taagtaagat
     7741 tccagaagaa agacaaacaa ctttcttttc agctactatg ccaagaggaa ttttggaatt
     7801 aactaagaga tatcaaaaag acccagagca tataaaagtt gttagaaaag agcttacagt
     7861 ttcaaataca aagcaatact atatagaaac taggtcatca aataaattag aagtattatg
     7921 tagattagta gatgtatatg accctaaact atctgttgtt ttctgtaata ctaagagaaa
     7981 agcagatgaa ttggtaggag atttacaagc aagaggatat tttgcagatg cattacatgg
     8041 tgacttaaaa cagactcaaa gagatatagt aatggataag tttagaaatg gtacaataga
     8101 tatacttgtt gctactgatg ttgcagctag aggtatagat gttgatgatg ttgaatgtgt
     8161 attcaactat gacttaccac aagatgaaga atattacgtt catagaattg gtagaactgg
     8221 tcgtgctggt agagaaggta tgtcatttac ttttgtattt ggaaaagaaa tgagaaagat
     8281 gaaagatata gaaagatata ctaaatctaa actaataaag cataatatac caacaataac
     8341 tgatgttgaa gagaaaaaag taggtacttt ctttgcacaa gttaaacaaa ctatagaaga
     8401 aggtcactta actaaacaat tacaatggtt agaaggtttc tgtaatgatg aagattatgc
     8461 aatggttgat atagctgctg cacttgtaaa actatctcta ggtgaagaaa tgaaagaaga
     8521 aataatagaa gaaaaaccta gaagagagcg tggagataga aaaggtggta ctggagctaa
     8581 agatggtatg attagattat ttatcaatat tggtagaaat caaagagtac aagcaaaaga
     8641 tatagttggt gctatagctg gagaagttgg aataccaggt aaagtagtag gtactataga
     8701 tatatatgat aagtatactt ttgttgaaat acctaaaaaa gatgcaaaaa cagtaattga
     8761 aaagatgaaa gatattaaga taaaaggtaa taagattaat atagaaaaag caaataagaa
     8821 gaaaaaataa cgtatagtat acaaaactat aattagaaaa tatataaaat aatatattgg
     8881 atatttaaaa agcctgatgt gttttcaaaa cagatagtag ataatctgta agagttaaca
     8941 gcataaggct tttcttgttt tatataagga atctgaataa cttactgtta aatctttgaa
     9001 ttactgcacc tcatttacgt taaggatgga gtattctacc tatttttgat aaaatatatc
     9061 tatgttttat atactatagt tttgaacata gaaagtttaa taaattatag tatatatgtt
     9121 aatttttaat aaggtaagaa agtagaaaga aggatatttt atggatataa taaaaaaatt
     9181 aagtcatggg tgtacattag attgtcatga ctgttgtaaa ttcaatgttt atactaaagg
     9241 aaataatgta gtaaaaattg aaggagataa aaatcatcct tatacaaaag gttttatatg
     9301 caaaaaaggg atggcccatt tagatagatt aaatcataaa gatagaataa agactcctat
     9361 gcttaaagtt gatggagtat ggaaagagat ttcttttgat aaagctatag aaataatggc
     9421 agaaaagctt acatactata aagaaaagta tacatcaaag tctgtcatgc attatgacca
     9481 atatggaagt gggtctgtgc taaaatacat aggagatata ttttttaatt tttatggagg
     9541 tgttagcaga cataagggtg gtccctgttg gagtgcaggg atgcatgctc aaaaatatga
     9601 ttttggagta gctaagagtc atgctataga agatatgtta aatagtaaaa gtatatttgt
     9661 gtggggaaaa aatccagcat atactacaat tcatactatg caaataatta aaaaagctaa
     9721 agaaaaaggg ataaagatag tagtgattga ccccatatat acaaaaacag ctcaaatagc
     9781 agataagtat gtacaagtaa atcctggaac agatggagct ttagctatag ctatggcaaa
     9841 aattatagta gaagataaat tatatgatga agaatatata aactcgtatg ttattggttt
     9901 tgaagaatat aaaaaatatt tatcttcatt ggaattaagt tttttaattg atgaatgtgg
     9961 tgttaaagag aatgatataa gagagttagt ggatttatat actaacaaat attcaagtat
    10021 aaatgttgga tatgggcttc aaaaatataa aaatggtgga aacacaataa gagcaataga
    10081 tgctttaggt gctataactg gtcaaatagg atttagtggt ggaggggtaa actatgctaa
    10141 taaagtttat ccgagtgtta taaattctga cccttacaat agccaatcat atggtgaaga
    10201 tagggagttt tatgtaagta atatcagtaa atttatagaa gaatctttaa aaaatacatc
    10261 aaataaagta aattatgcat cagatgaact tgatatggca ttacataaag taaattacat
    10321 atcagatgaa cttgatacaa catcaaataa agtaaattat gtatcagatg aacttgatat
    10381 ggcattacat aaagtaaatt gtgtatcaga tgaacttgat atggcattac ataaagtaaa
    10441 ttgtgtatca gatgaacttg atatggcatt acataaagta aattatgtat cagatgaact
    10501 tgatatgaca tcaaataaag taaattatgt attagatgaa cttgatataa catcaaataa
    10561 aacagattat atatcaaatg aattgtataa tttatctaat aagtcaatca aagataatat
    10621 tcctataaag atggctgtga taactaaaag taatatgctg aatcaactgc cagatttagt
    10681 agaattggag agggtgtttt caaaaattga atttaaagta tgttttgata tgtttataac
    10741 tgatacagct actttatgtg acatgtttat accgtgtaca aatacactag agagtgaaga
    10801 tataatttat agctcaatga ccaatccata tataacctat aatgaaagag ctgtaaagcc
    10861 agcacataaa ttgatggatg aatattactt ctttatggag ctagctaaaa agatgggatt
    10921 aaatgattat ccttttgtag aaaagagaac ttatttagaa aaagtgattg aaccacttaa
    10981 gcgttttgac aaaaatctgg atatagaaaa acttaaaaac aattacttta ctatacataa
    11041 ccctgttgct tgggaagata agaagtttga gactccatct ggaaaatacg agctttattc
    11101 agaaagtata aaaaacttgg gaataagtcc aacacctgta tatataagca ataaatataa
    11161 agaaatagaa gataaaaata tttcttttag attattaact aatcatcatg cagatacttt
    11221 atttagtcag cattttatgg acaaaaaatc tatagctcaa gcatatataa atcggagaat
    11281 ggctaaaaaa gtaggtattg aggacaaaga tattgtgata ttgaggtcaa aaaaagcaaa
    11341 aataaatgta caaataaata tagatgatgg agtaggtaat tatatagtaa aaatgtatgt
    11401 tggttggtgg aagaaacatg gaaatcctaa tagtttaaca gacactggta tatcagattt
    11461 tgggggtcag gtaacatata atgaaagtat ggtagaaata atacgacaaa actagttaaa
    11521 tttcattgaa ggaataaaaa tataattata gaattgatta aaagttacta tttttacaaa
    11581 actattgaag gagggatttt tgatggtaaa ttattcctta gaacataaga atctatacat
    11641 agaatttatg tgctcagaac tagaccacca tgtagccaat gaaataaggg aagaaataga
    11701 taacttatta agtgtaaatc aagtaaaaaa tgttgtattt aattttgaaa atattaattt
    11761 tatggattct tcaggtatag gcgtcatcat aggtagatat aaaaagattt ctaatgaagg
    11821 gggacgagtt tcagtaatta atataagttc acgagttaag aagatttttg atttatctgg
    11881 gttaaataaa attattggca tttatgatac gtatgaagaa gctctaagtt ctttgtaggg
    11941 aggagtatgt gatgaataat attatggaag ttaaattttc ggcaatatct gaaaatgaaa
    12001 gtttagcaag agttatagta gcatcctttg cagctaagtt agacccaaca gtagatgagt
    12061 tagtagatat aaaaactgct gtctctgagg ctgttacgaa tgctataata catggatatg
    12121 aagaagacag ttctaagttt gttttcttaa gatgtgagat agaaggaaat actataaaga
    12181 ttatagttga agatgaaggg tatggaatag aaaatgtgga gaaggcaatg gaaccacttt
    12241 atacatcaaa accagaactt gatagatcag gaatgggatt cacagtaatg aaaagcttta
    12301 tggacgatgt agaggtaagt tctgtaaaag ataatgggac tagaattgaa atgactaaaa
    12361 aaataaatgt gcctaaataa agtccaaaag tgaggtttta ttatggaagt aactgttgcc
    12421 agagaagaaa aaaaacctct tcttagtcat gaagagactc tagaacttat tgaaaaagtt
    12481 caaaatggag atgaagaggc aaaagaaata ttaatatcaa gcaatttagg tctagttagg
    12541 agcgttgtat ctagattttt aaatatagga tatgatagag aagatttatt tcagttaggt
    12601 tctataggct taataaaatc aatatataaa tttgacccaa aatttaatgt aaaattctct
    12661 acttatgcag taccaatgat tttaggtgaa ataaagagat acttaagaga tgatggtatg
    12721 ataaaagtat caaggtcgtt aaagcaaata gctgtaaagg caaagatgga aagtgaagct
    12781 ttaactaaaa aattaggtag agagccgagt atagaagaat tagctaaagc aatagatgta
    12841 gaaaaagaag atttagtaat ggctatggaa gctaatttca atgttgagta tttgcaagga
    12901 gttattcatg aagaggaagg ttctccaata tgccttattg ataaaataag catgaaagga
    12961 gaaagtgaag aagaaaaagt agtggataat atattgttaa aagacatact tggaagactg
    13021 gataagagag agcgtcaaat aatagttctc agatactttg aagacatgac tcaaagtgaa
    13081 ataggagaga tgctcaatat atcacaagtt caagtatcta ggatagagaa gaaagtattg
    13141 tctaagttaa aagaatatat atcgtgataa tacttatgat atgtagaata acaaaatata
    13201 ataaatatat tactaaaata aaatgtaata actttttatt ttaagttttt aaattattaa
    13261 aagatattaa aataataaaa agcattaaaa aagtaaaaat ataaaaacaa ggtgtcttta
    13321 tggaaaaata gattttttct ttattaggca tcttttttat tttttgacca aactttagat
    13381 agtcaaaata aataataggt ttattttttt aaaattaaca aataatactt tagagaggtg
    13441 tttagtatgg ataaaaatta taaaaaatat gtagaccaaa taagcccaaa acctacatat
    13501 ttaaaaaact atacacttgc atttattgta ggtggaatta tttgtatgat agggcaagca
    13561 ataaatgatt tgtatatgaa agttggaggt cttgataaac taggtgctag ttctgcaaca
    13621 tcaataacat taatatttat aggggcattt ttaactggat taggagttta cgacctaata
    13681 ggaaaaagag ctggagctgg ttctataatt ccaataacag gttttgctaa ctcaatagta
    13741 tctccagcaa tggagtataa aagagaaggc tatgtattag gtgtaggtgc aaacttattt
    13801 aaaattgcag gaccagtttt agtttatggt ataggttctt ctattttatg tggaattatt
    13861 tattatattt ttaagatgtt ttagatgatt tttatttaat ttaaatttta ttaattatga
    13921 agaaggtata aaatctgaaa atagctacta aagtaactaa ttgaaaaata attgtgcaga
    13981 tttaattaat atttttaatt aatattaaaa atttgaatag taaaatgcaa aattaaaaag
    14041 gtgtgaaaat atgaaaaata aaagaattgg aaaaagaaca gtcaagttag aaaataaacc
    14101 aaccataata tcaactggaa ctatagtagg tcctaaggaa ggtgaaggac cactaaagga
    14161 ctattttgat atgattatga ctgacgattt atatggtgaa aagacttggg aattagctga
    14221 aagtaaaatg gtagagactg catctcaaca agctatccaa aaggcaggta aaaaattgtc
    14281 agatgtaaat tatatgctag gtggcgattt aataaatcaa atagttccag catcatttgc
    14341 agcaagagaa ctggctattc catttttagg tatatatgga gcttgttcta caatggcaga
    14401 aggtctttgt ataggttcta tgcttataga tggtggtttt gctgatttag tactgtcagg
    14461 tacatcaagt cactattgta cagcagaaag acaatttaga ttcccacttg aacttggaaa
    14521 tcaaaaacca atgacagctc agtggacagt tacaggagca ggaagtgtat tattagctcc
    14581 taatggagac ggtccaaaag taaagtatgt tactgttggt aaagtaatag atgagggtat
    14641 tgatgatggt aataatatgg gaccagctat ggctccagca gcaatagata ctatctactc
    14701 ttactttgaa gatactaatg atgacccaaa tagctttgat ataattgcta ctggagattt
    14761 aggtactctt ggaaaacaga tagcagaaga ctttttaaaa gaaaagggtg tagatatatc
    14821 aaaagtttac actgactgtg gtatagagat gtttaacctt aaagaacaag atgtccattg
    14881 tggtggaagt ggatgtggat gttcagctac agtgtttgca ggatatatat atgataagtt
    14941 gagaaaaaaa gaatttaaca aagtaatgct tgtatcaaca ggagcactac tttcaccgac
    15001 aagtacacta caaaagcaga caataccatg tgttgcacat gcagtagtaa tagtaaatga
    15061 gtaggaagtg atataatatg ttaatggact atgtaagagt atttattgta ggtggaatta
    15121 tatgtttaat agcccaagta atgatggatt atttcaaaat gcaaactcca tatgttatgg
    15181 taacgtatgt aacaactggt gtagttttaa cttttctagg actttatgag ccattagtta
    15241 agtttgcagc agcaggtgca acagttccta taataggatt tggatattca cttgctacag
    15301 gagtaataaa atcaattcaa agtgatggtt ttttaggtat ttttttagga ggtacaactg
    15361 ctacagctgg tggaatagca gcagctatat tctttggata tattatgtca gtagtattta
    15421 ctccaaaagc aaaaccataa atctttatta aaagaataat aagtttttga aataataact
    15481 ttttataaag caggtggata tcttttagat tccacctgtt ttttatataa agaagcgttt
    15541 acataattga agttgacaat tatttacaac atagtgtagt attatattat caaaataaaa
    15601 tgaagtgaag taggggataa agatggtaac tattaaagat attgcaagga atctaggtat
    15661 cagttattca actgtttcta ggtgcttaaa caataatcct aatgtatctg aaaaaacaaa
    15721 aaagaaagtg gtagaagagg caaatagact agggttccat tttaatgtaa atgcaagaaa
    15781 tttagcaaaa aaagaaacta acaggatagg ggtaatcttt tcaaataatt ttaatcatca
    15841 agatactaga aagtttttta gtgatataat ggatagttct ataaattcta ttgagactaa
    15901 taaatacgat tttataatac agcctaataa taacattagt ggtgatagta atgtatataa
    15961 aatggtaaat ggacaaatgg ttgacggatt agtaattgtg tctcaatcaa taaaaaaaga
    16021 agagtatgat tttttaaaag acaataattt tccacatgta tttatatatt ttaaaccatc
    16081 ttttatagga gaagtagaca atttcttttg ggacgataac gtgtatggag gttatatagc
    16141 taccaagcat ttaattgacc atggtcataa agatataata acagtaactt cagatgataa
    16201 gagttcaaaa atgcatgaag atagaaccaa gggatatttg aatgctatgg aagaggcaaa
    16261 tttaaagaca gaagtcatta aatctaaaat ggattttgaa tctcaagttg aatttttaga
    16321 aaaatacatt gataaaataa aaaaagcttc agctatattt gtacaacaag atgtaccagc
    16381 aatttctata atacaagagt taaaaactac ttatggtata aatgtaccag aagatatttc
    16441 cataataggg tataataata ttgaattaat atcatatttt aggtcgcatt tatcaacaat
    16501 agatgaccca agagagcaag ttataaaaaa tggagtagat agtttagtaa atataataaa
    16561 taaaaagagt acagaacaca ttccaagaaa attatatcca aggttaataa tacgaaatag
    16621 cgttaaaaga ataaagtagt aagcttttaa gtatcaaaag tttattaaaa atattataga
    16681 ttatgtgaag ttttttaaca aataatataa ataaaaatta aaaggagtag gtgtatataa
    16741 gtacattgta ctcctttatt tattgagata gtttttataa tatgaatgaa taattttatt
    16801 caattatttg aaaatccgtt attttaacaa tataaattaa taaattattt gtattttata
    16861 tcatgaatct ataatatttt tttgattcat aactaaaagt atataaaaat gatgaataaa
    16921 tatatattat acattatcaa tgataggtta tataataaaa tcactaacaa ttatattata
    16981 agaaaattat aaaaattaca aggaggtata gaaatgaaag aaaaagcagg gattagaaat
    17041 gccataatag cagcctttat aatcacaata ttggcagaat taattggacc tatttcattt
    17101 aaagttatgg gaattaatgt aactttactt agtatactat gggctatatt cataggaatg
    17161 gctgtttctc ctcatttatt agggagagtt ataccagcct taaaaaagtt tataggggat
    17221 aatgaaataa atgtatctcc atatttatta tctttgacac tatatccatt agggataatg
    17281 tttggaatta atgcaggacc taaagttggt atattacttc aagcaggacc agcactttta
    17341 ttacaagaat ttggtcatat gggtacaatg ttgatagcat tgcctgttgg agtagcatta
    17401 ggtcttggaa gaagtgcatt aggtggtact ttttcattat gtcgtgacac agcactagga
    17461 attattggag ataagtatgg attaaactca ccagaaggta taggaacact aggaacttac
    17521 ataagtggta gtatatttgg aacattatta ttttcatttt tagcaccagt gggtgtactt
    17581 ttaggatttc atccatatgc tttagcaatg gcatcaggaa tgggtagtgg ttcaatgatg
    17641 ggagctgcaa cagcatcatt aatgaatacg gttccacaaa tgtcagagca gattttagca
    17701 tattcagcta ctagtggatt gattacagct gttactggaa tatatataga attattctta
    17761 tctttaccag tagccaacta ctattatagc aaagtagctc cagttattga aagatttaga
    17821 ggaagaaaaa caagaagaac aagtaataaa ttgtaaagga ggatatatat atggaaaaaa
    17881 cttttaagac tattatggag cagataaaaa tagtattgtt tatatatatt attatttgct
    17941 ttggacaaat gatagcactt aagactcagg ctcaagattt gttgattgga agtgtatctg
    18001 gatttttgtt ggtgataatt gctattataa taaagaattt tgttaaaaaa cctaatttac
    18061 caggttttgc atgggctaca ttagttgcat tcgttttatc gcttccaata tctccagtgg
    18121 gggatatcat agcaacaaat gtagggaaaa tcaattttat gtctacagta acaccattat
    18181 tagcttttgc aggaatctct gtaggtaacc aattagatat attaaagact ttgagttgga
    18241 agttagtagt tatatcattt atagttatga catctactta ctttggttct gcttttatat
    18301 ctcaaacagt tttaagatta aatggaatgc tttaaggagg tagtaaatat gaatgaatta
    18361 aaagcaaaaa ttaaagagga aataaagtta ttatctgaag agaaaaagac atcatttgaa
    18421 aaagtttctg actatttatt ttcaaatcca gaattagcat ttgaggagta taagtcacaa
    18481 aaagcattat gtgatttgtt agaagaaaat gaatttaatg taactaaagg tgtaggagga
    18541 ttagaaacat cttttgaagc tgtgtattca aatggaacta atgggaaaac agttgctttt
    18601 ttagctgaat atgatgcact tccaggaatg ggacatgcat gtgggcacaa tatcataggg
    18661 acaagttctg ttggtgcagg tatcatatta aaagaaatca tgaaaaaaca taatatagaa
    18721 gggactgtaa aggtttttgg aacacctgca gaagaaagag taggtggaaa aataactatg
    18781 attaaagaag gtgtatttaa taatgtagat gctgcattaa ttttacatcc aagtgatgct
    18841 tcaatgcctg atgatatatc atttgctcaa gttaatttaa aatttgattt tactggtaag
    18901 gcatcacatg ctgctgcttt tccttgggaa gggaaaagtg ccttaagtgg agtaatagca
    18961 ctatttaaca gtgtaaatag catgagatta catcttaaag attatgcaag agtacatgga
    19021 attataactg atggaggtag catacataat ataattcctg aaaaatctac agctatattt
    19081 aatgtaagag ctttaagcat agaatattta aatgagattt gtgaaatgct taaaaattgt
    19141 gctaaaggag ctgcaatttc tactggaact aatgtagaaa tagttcaatt agatgaaata
    19201 tacaaagaga ttaagaatga ttctgaatta gtgaatatag ttagacaaaa ttttgaagtt
    19261 ttgggtgaag actatgttga aagagattta agtcaaggta taggttcaac tgatacagga
    19321 aatttaactc atgagatacc tgcaatacaa gcatatataa aattaaaaga aaatactgca
    19381 actcatacag atgaatttgc tgttgcagct ggtggtgaag aaggcagagt agcacttata
    19441 aaagcaatta aggtgttagc aatgtgtgga gtagatattt tgtatagtaa ataatatata
    19501 attttaaaat atattaagaa aggaaatcgg attagacatg atattaaaac aagtaataga
    19561 ggtttatgac attttagata aagcaaatgc taatggggaa gaagtaaaaa cttacttaca
    19621 aggttatggt gaagtagatg taactgtaaa agaattgtct tcaagtaaag gttcaacaga
    19681 tttagtaaag cttacaattc ctggaaaaaa tggaaaatta aaaggtggag atgctcctac
    19741 tcttggtatt ttaggtagac taggagggat tggagcaaga ccagaagtta taggttttgt
    19801 ttcagatggt gatggagcat tggtagcaat tgcagtggct gctaaacttt tggatatgca
    19861 acgaaaaggg gatatattag atggtgatgt tgtaatttca actcatattt gcccagatgc
    19921 tccaactaaa gaacactatc ctactccttt tatggattct ccaattgata tgatgactat
    19981 gaatgaaaat gaagttgata gtagttgtga tgcaatactt tctatagata ctactaaagg
    20041 aaatagagta atcaatacta gaggatttgc aatatcgcca actgttaaag aaggctacat
    20101 attaaaaact agtgataacc ttttagatat tatgcagact gtaacaggga aatctccatt
    20161 tgtattccct ttgtctattc aagatataac accatatgga aataatctgt atcacttaaa
    20221 tagtatttta caaccagcag tagctacaga tgcaccaata gtaggagtag caattacaac
    20281 agagatgcct gttgctggat gtgctacagg tgcaactaat tttagtgatt tagattcagc
    20341 tgggagattt gcaattgaag ttgccaagct atttggtaga aataagtgtg atttctatga
    20401 taaagaagag tgggaaatgc ttattaagag atatggcaaa ttaaatagat ttcaaacatt
    20461 tggtatacag taattagaaa aatattaagt ttatataatt tgtgatataa ctatttaaga
    20521 atatataaac aattaggaat aaaagtaaga ataaagcact tagttaaaaa ctttaaaaat
    20581 aaaatattta tttgtttata taaaaaagga ggattctggt tattgggatt ctctttttta
    20641 tgaatacaaa taatatattg taattataaa aaaaggagcg tttcaaagtg atttttttaa
    20701 tcaccttgag acaactcctt atatttttat ttaaattcta ttttttatta agttgctgga
    20761 gttccaccac catcaggagg agtagtagag ccaccaccat tatttccacc accatcagga
    20821 ggagtagtag agccaccacc attgtttccg ccattatcag gaggagtagt agagccacca
    20881 ccattatttc cgccattatc aggaggagta gtagagccac caccattgtt tccgccatta
    20941 tcaggaggtg tagaaccgcc accattatca cctgtgttat tattgttatt attattttct
    21001 tctggttggt tattgtctgg tttttttgta tcttcagatg atggttgaga acttacttta
    21061 gtaggagcag taccttcaat aaatgctgct ttagagccag aagattgctt tcctccatcc
    21121 tctaaattaa tttttgtaaa atatacattt ttaggaactt caaattcagt aaccgttaaa
    21181 ttagcatgta ttttttccat aattttttcc catagttttg cagtactagt acttccatgt
    21241 ggtacttctc tacgttttat agtgttacca gaatcatctt ttctaccagc atcatctcca
    21301 atataagttg caccaacata ataaggagtg taaccgacaa accaagcatc atatgcacta
    21361 tttgtagtac ctgttttacc tgctacaggc attccctttg gaagtgtagc aacaccacca
    21421 gtaccttcag taattacgct ttctaacata tctgtaacaa cataagctac ttcagggtct
    21481 acaactttat gttcttcagg agcattttta actaataatt gaccatcaaa ggtagttata
    21541 gtagtaaaaa tagaaggttc tacataaata ccgtgatttg ccaatgtacc ataagcagca
    21601 gccatttgaa gtggagtaat accattcgcc ataccaccta gtgtcaatga tgggaatttt
    21661 ctatcacttg cttcagatga acctgagtga ttattcttta aagttgttat accaaagttc
    21721 tttaagtaat ccatcattat atctacacat tcatcataag agtctccaag catttcagca
    21781 gttttgacag ctatcgtgtt tgaagattta gcaagtgctt tacgtaaact catagaacca
    21841 tgaccagcag tagtagttct tggattccat ttattatttt cttcaaactt atatcctcct
    21901 cgagaatcac ttagagcagt tgcttgtgtt atttttagag tatcaatagc tggcgtatat
    21961 attgctaaag gttttataga agaacctggc tgatgagggt tagtagcacg atttaaagtt
    22021 tttctaccac ttatatttct tcctccagct agtgctctta tttcaccatt tttataatct
    22081 aagattacca ttgcagcttg cggttggcta actccatcag gaccgaccat atgtcctggg
    22141 aaattgctat ttctatcaaa ttcttcttct aaagcatctt gcattttagg gtctatagtt
    22201 gtgtttacaa ttagaccacc attgtagaat aagttttgag cttcatcatt tgtatgtcct
    22261 tgttccatta aagcatttat aacttcactt tcgataaggt cttctacaga tgaagaaact
    22321 ttatctgatg attgaggcaa ttttatttct attttagctt tgattgcttc atcatattca
    22381 ctttgtttta tatatttcaa ttctaacatt ttctttaaaa caatttcttg tcttttttta
    22441 gcttcaggat tacttacagc ctttctaaca actaatgtac ctgcttttaa ttgtctataa
    22501 gtatcattag atataagctc ccaagatttt attttttcaa tcatatcaaa gtccacttga
    22561 gtagggtcat ctaaatcatc atcagttgtg ttaacaaaaa ataataattt attctctaaa
    22621 tcctccttag tttcatttcc ctctaactta gaagttttat aggctgaaaa tcttgatgga
    22681 tttttagttg aaccagcaag aagtgctgac tctgctagag taaggtcagc tgcagactta
    22741 tcaaaataac cacgtgcacc agcctcagca cctgctagac ctctaccaac gaagaagtta
    22801 tttaaataag tttctagtat tttatcttta cttactgttt tactcatctc atgtgcatag
    22861 taaatatcct ttattttacg aggtatagtt tgctgttcag ttgttaacag cattttggat
    22921 acctgcatag gaatagtact accaccttgc ttagtacctg taagggtctt taatactgac
    22981 ctaaataaac ctttaatatc gacaccctta tgctcataaa aacgctcatc ttctattgca
    23041 attacagcat gttgaagata aggggaaatc ttatctaatg gaacgggtgt ctttttattt
    23101 acacttggag ctgtagataa tgtttttcca ttagcgtatt taatagtagt tgtttggttc
    23161 gtctgtttat ctaaaacagc ttctgtaact ggagttacat ctcttagtga cgcaaatact
    23221 agaccagcag tacctgctgt accaacaact agtaaaacta aaaatactat cccaaatact
    23281 cttagttttt taaatttatc gcttttttta gattttttct tgttttttgt tgaacgagtc
    23341 ttgttagctg aagttctatt aattggttta tttgtattac cagatgagct aacttttttt
    23401 ctcctgattt tatttccatt tttatcatta tcgttattca taagatttta acctccttac
    23461 ttaatgcaat attataacac aaataggagt agttttgtaa ttaatagaaa aaatgaattt
    23521 gctataatat tctgtacatt gtatatgtat aaacgtaata tattaaaaag tctatatttt
    23581 tattttattt atttttgaca aatattataa aaattatgcc aaagctacat ataaataaat
    23641 aggaggggat aattttgaat agaatagtag atagaaaaac aaaaaatgaa tttagaaaaa
    23701 tcatagctat ttttttagta attcttttct tgtctgtatt tataggcagt tttatatata
    23761 tagataaaac tttaagaccg acaataacag ttttagcaga gactaaagct cttgaactag
    23821 caaataggtc gataaataaa gctgttgctg agatggtaga aggaaaaata aattatgaag
    23881 atttaatgga tatacaactt gataataatg gtaaaataac tatgatacaa gcaaacacta
    23941 taatgatgaa tgaaattgca tctgcaatag ctttagaaat acaagatgaa ttaaaaaagg
    24001 ataagacagc aagttcttac atacctatag gaacagcatt aggaagtcct atattagcta
    24061 aatacggtcc taaacttgaa gtatctattg aacctatagg aacagtatca gtaaacttta
    24121 aaacggaatt tgagtcttca ggtataaatc aaactagaca tagaatatat ttagaagctc
    24181 aaacacaagt taaagtagta ataccactta taacttcaac aaaacaaatt aaagctcaaa
    24241 tacctatttg tgaaactata atagtaggtg atgttccaga aagttatgta aatattccag
    24301 aaaaaaatct aggaaatgtt ttacctaaca ctggaaagaa taccaacaag taatataaag
    24361 ttgtatattt ttaaataaat tcaaaataat agtttataaa taaaattttt ggaaataata
    24421 ttaatataat attaataaaa atttatattt tatactatgt aaaatatcta atatatttgg
    24481 tctgtaggtt aaataaaagt acctttagac caaatatatt attaataact taataacatc
    24541 ttaaaactta tttatttata ttatttacaa catccattta ataaatacac cataacctac
    24601 agctgggtcg ttttcaattg catgtgacac tgctccgatt attttattat cttgaactat
    24661 tggagtacca ctcatacctt gtacaatacc accagtttga gctaaaagct ttttatctgt
    24721 aattttaatt ttaaaacttt tactttctgg atgtttttgg ttttctatat ctataatttc
    24781 aatattatat tttttacact tattaaagtt atcttgtaaa attatttgag cttttcctaa
    24841 tttaacttca tctaaagatg ctactttata tttttttaac ttggaaatat caaagttatt
    24901 aatttgacct cttattccaa aatcagtatt ttctgtaaag ctaccaataa tattttctcg
    24961 ttttgcattt atacatccaa cactaccttt ataagatttt tctatattaa ggtgcttagt
    25021 tgtagaaatt gaacctgttt taataggtat ttttcttgca ttaccaacac ttattggatg
    25081 acctacagct ccaaactctg atgtatctgg attgatatac gttaaagtag caaatccaga
    25141 aagtaaattg ttaaaactaa ttttttctaa tatgtgtttg gtagttttca ttgttataag
    25201 ttgtccacct ctatttaata aaatagatac ttgtttttct tctggtaaag catttaaggt
    25261 attggcaaag tcactatatc catcaacttc agcattattt acctttacta tagcatcacc
    25321 taatctaaat ggagatgctt ctccaggatt tctaactatg atatttttta attctgcgtc
    25381 aatttgaagt acgtttccaa ttggtattaa atattcaggt tcattttttt gtaaattttg
    25441 tgcataaatt aaattatttg agaaaaaata taataaaaat aaaaatgtta aaacaattgt
    25501 aaattttgac ttagctaagt tggttttttt acttaaagta ttatttttaa aatgaaaatt
    25561 tttaagttgc ataaaacacc tctttcttat taaattattt taataataga attgaagttt
    25621 tcttgctgct tctatattat gttgtgcaag aatcatgttt tgatgcttgg aaaactagct
    25681 caataacacc ttagttttca taaaaataaa taaaataaat agtgtatatg ataaaataaa
    25741 tacatattca tataaataaa aataaacaat aactgtctag cataagaaag gagcaaaaaa
    25801 tgaaaaaata tagattgtta gttacttgta taattgccat gggaatcata attataggaa
    25861 catcaaatcc agttagcaat ttttacaaga ctacaaattt gattagttca ggaaatatca
    25921 caaatgaaaa agcagataaa gatgaaaatt caaacaaaaa taataaagat aaaaatgaag
    25981 acaaatctaa ttctaaaagt aataaagata agtcatcaga ggacaaatcg tcagataata
    26041 aaaatgaaat tcaaaaaaat aaaaaacttt taatttgtat agacccaggg catcaaggaa
    26101 aaggagatag caatttagaa ccagtagctc caggttcttc ctctaaaaaa gcgagagtat
    26161 cttcaggaac agagggtatt gctacaaaaa aaccagaata tgttttaaac ttagaagcat
    26221 ctcttgtttt aaagagtata ttagaatcta aaggttataa tgtaataatg actagagaga
    26281 cacatgatgt aaatataagt aattcagaaa gagcaatact tgctaatgat aaaaaagcag
    26341 atatggttgt aagaatacat gcagatagtt taaataattc ttcaaaaact ggagcatcta
    26401 tattgattcc tgaaaaagat ggaaaatata cagctcctat atatgaagaa agtaataaat
    26461 gtgctgaatt tatcaaacag aatatggaac aatcaggtat acaaatcaat ggaatcgttc
    26521 aaagaggtga tttgactgga ttcaactggt caaaagtacc agctgtatta gtagaaatgg
    26581 gatttatgag taactataat gaagaccaaa tgatgtcaaa tccagactac caaagaaaaa
    26641 tgatgcagtg tatagctgat ggattggatg cgtattttaa ataagatatg aatagagaaa
    26701 aagttaggtt ggtattattt tcctaatttt tatctctatt ttttattgta cataacacaa
    26761 agaagaagtt tattttaggt taaattgtcg taaatataac taaaaatatt gggaatactt
    26821 gatttaagtt taaaaagaat atattatagt atagaatgtg taaaattttt ttgagaggtg
    26881 ttgaaatgct agtagtagaa aatgtgagtc atggatttgg tgcgagaaca atattagaaa
    26941 atgtatcctt tagacttaga aaaggtgaac atattgcact agttggtgct aatggagaag
    27001 gaaaatcgag ttttttaaat ataataacaa agaagcttat gccagatgct ggaaatataa
    27061 aatggtcttc aagagctaca gtaggttact tagaccaaca tacggtatta agcaagggaa
    27121 aaactataag agaagtgctt agagaggctt ttaagcatat gtttgactta gagcaagaaa
    27181 tgattgctat gtatgacaaa atgggagaag ctagtgatga tgaaatgagt aagcttcttg
    27241 aagaaacagc agagatacaa actatacttg aaaatagtgg tttttatatg atagatgcaa
    27301 agatacaaga agttgcaaat ggtcttggtc ttggagaaat tggtcttgat aaagatgtaa
    27361 ctgacttaag tggagggcaa agaacaaaag tacttcttac taaacttcta cttgaaaatc
    27421 caactattct tatattggat gagcctacaa actatttaga cgaagagcat ataacatggc
    27481 ttactaagta ccttcaagag tatgagaata gttttgtctt agtttctcat gatattgaat
    27541 ttataaataa tacatgtaat gtaatatatc atatggaaaa tggagagtta aatagatata
    27601 aaggaaatta tgatgaattt gtaagactta atgatataaa gaaacgtcaa gaagaacaag
    27661 cttatgataa acaagttgaa gaaagaaaaa gactagagga ttttgtagct agaaataaag
    27721 ctagagtagc aactagagga atggctaaca gtaggcaaaa gcaacttgat aagatggaaa
    27781 tattagaaag accaaaagaa aaaataaaac ctacttttgc ttttaaagat gctagagcag
    27841 ccagtaaaat aatttttgaa acagaaaatt tagttttagg gtatgatgaa gcattgacaa
    27901 aaccacttaa tttccattta gaaagaggaa aaaagattgc cttaaaagga atgaatggta
    27961 ttggaaaatc tactctatta aaaactttac ttggaataat aaaaccattt gaaggaaatg
    28021 ttaaattagg tgactatttg gaagttggat attttgaaca agagagttca agagaaaata
    28081 gtaatacacc tatggatgaa gtttggagtg aatttccagg gcttacaaac tttgaagtta
    28141 gacaggctct tgcaaaatgt ggacttacaa atgaacatat aacgagtcaa atgagagtat
    28201 taagtggtgg agaagctgct aaggttagac tttgcaaagt aatgttaaaa aatataaatt
    28261 tcttagtatt agatgagcct acaaatcatt tagatgttga ggcaaaagat gaattgaaga
    28321 aggctatcaa agagtttaag ggaacagttc ttttagtatg tcatgagcca gaattttatt
    28381 cagaaattgt tgatgatgta tggaatatag aagattttac aactaaaatc gtgtagtgat
    28441 ttaacttatt gctgggggga tttcatgaga aagtttctaa atgtactttt ttcagtttgt
    28501 attagtattg ttataatagt aggtgttatt aattttactg ttggatttaa acaactttat
    28561 tattttgaca ttgattactt aaatatatca gagttatctg gtttatctaa agatgatata
    28621 aaattgaact acgattatct aattgattat aacttaaata agaatgtgtc tgagttcaaa
    28681 ttaccaacat taaaatcttc tccacaaggg aagatacatt ttgaagaagt caggaatata
    28741 tttcaaaata taaataaatt agctaaatta ttattggtag tttctttagt aggaattata
    28801 ttgagtgtaa aaaataaaaa tataaaaatc ttgaaaacta catcaataac acttataatt
    28861 atgccattat tattaacagt tccaatttta ttaaattttg aaaagtcatt tattattttt
    28921 cataaattat tatttagaaa tgattattgg atatttaatc ccgatttaga cccagttata
    28981 aatatattgc cagaggaatt tttttttcac tctggaatga tgattttaat attaattttg
    29041 ttagtaagta tattactgtt tgtaatgtat aaattatgta aatctttatc aataaaaata
    29101 tttcaatgtt aaatagaggg atatttttat ttccaaaata agataataaa aaaggattag
    29161 attaaaagtt atacaataaa aatagctgtg caataatatt tagactatga aagtagttaa
    29221 aaaatattta tgcacagcta tttataatag aatttacttt ttactagtga ttttaactaa
    29281 atcttgaaat ttactcataa tgtaattgta attatctttt ttatgaggta acatacaact
    29341 actgcaatct ttgatacctt tatctgtata cttaaagttt ccaccacagt tttctcctaa
    29401 agcatacaaa ggacaatagc aaaatagaca gttaaattct tctggtttat tagttttatg
    29461 acaagggaaa aattcgcaat ctttgtgatt aaaaaactta taattttcgc ccatattttc
    29521 ctcctaatta aataaaattt aaatttttta tactatataa gattttggtg ataatataat
    29581 tatactaaat aattttatga atagtcgata atatagtatt attaatcata gtattaatgg
    29641 tataattagt caacagaaaa ataatgtaaa aataatgtaa gtgtattttg taattagaat
    29701 atgttttata acaaaaaatg aggtgattaa gtgaaagaaa ttcaagaaaa agcacttctt
    29761 gtaggtctga atttgactac aatagtgaaa aaaaatgatg atatagacac taatgaatct
    29821 atggaagaat taaaagaact aacaaaagct gctggggcag aagttgtagg tagcttaatt
    29881 caaaataaac actctgtaga tgcagcttat tatataggaa aaggtaaagt agaagaaata
    29941 agagcctata gtgactcttt agatgctact ttagtaattt ttaatgatga attatctggt
    30001 gctcaaatca gaaatataga gaatgttgtt ggaagaaaag taatagatag aactacttta
    30061 attttagata tatttgctca aagggcactt agcaaagaag gtaaattaca agttgaatta
    30121 gcacaattaa aatatagatt gccacgactt tatggaatgg gtggtgagat gagtagaaca
    30181 ggtgcaggga ttggaactag aggtcctgga gaacaaaaac tagaaataga taagagacat
    30241 atacttaata aagcagcaga tataagacgt gaacttaaag aagtaaaaaa gaatagagag
    30301 acacagagag ttaagcgttt aaagtcaaat ataccgattg tagcacttgt tgggtataca
    30361 aatgcaggaa aatctaccct gttgaacgaa ttaataaaga cacacaagga ttatgaacaa
    30421 gaaaaagaag tttttgtaaa agatatgctt tttgcaactt tagatgtaac cttgagaaag
    30481 gctcttttgc cgaataaaaa agaattttta gtagtagata ctgttggatt tgtaagtaaa
    30541 cttccccatg atttagtaga agcgtttaag gcaacattgg aagaagtaca gtatgcagat
    30601 ttaatcttac atgtaattga tgcaacaaat actagttatg aattgcaaaa aagtacgact
    30661 gaaggtgtat tgaaagaact tggtgtaaat gataaaaaac atatacttgt atataataaa
    30721 gtggacaagc tagaattgga tatatatcct aaaagtcaag aagatattgt gtatatttct
    30781 gcaaaacaag gcataaatat ggataagtta ttaaatatga tagaaattgc tttaatggaa
    30841 aatacttatt cagtaagttt gatgttacct tatgagagag gtgatatatt tagtagaata
    30901 aaagataaat ataatgtgga gaattttgag tatggggaaa gtggtataac gctagatgta
    30961 gatttagatg aagaagattt taatatctat agagagtaca tactagaaaa ataggttgaa
    31021 tatggatata aactgggaaa atatagataa tcttgaagat tattttataa cctatcttct
    31081 ttataaagaa tctaaaacag tatctcaaat tagtaaaatt agaaatattt caagtactga
    31141 agcaagtgag caacttatac aagcaaagtt aaaaataaag gaaatgcaaa aagatgattt
    31201 tgaggcatct aaggatattt tggataaatt tctagaacta gataaaatta agagacttga
    31261 ttttatggat agcttagatg acgaaaaaat ggtatacttt aagagaaagg ttttcaaaag
    31321 aatccttgta gaaaaaaatg ctgaagattt aattgtttta atttgggcga caggtgagct
    31381 aaaagatgat aggtttttga aattgcttca tcagctcaca aaccacagac actcagatat
    31441 tagaagaata acttattcag caatcagaaa gattgaatca ccaagtagta gagaagtact
    31501 gcaaaaaggt ctctacgaca aaaatgcaca gactaggcag tactgtgcaa aagctctttc
    31561 aaaattggga gatgaaaaca gtttaaagat tttacagcag ttgaaagaca agaataagaa
    31621 ttttgaaaaa gaatatgtgc ttagagctta tgacgaagcc attaagtcat tggaaaatgc
    31681 aaaaaatttg aaaaagtaat tttttattaa agcaatttgt ttacttaatg atgtatttct
    31741 aaagtttaac aaggtaataa ataaattaat gggaggggtt tcaaatgata gtaagacgtt
    31801 gtgcaggtgg agtagtgttt tatgctaata aagtacttat tgtaaaaaat gataggggtg
    31861 aatggacact tccaaaagga aagatactag gagggggact tccatatgag agtgctgttc
    31921 aaagagttaa agtagaaact ggcatagatg ctaaaatgat agatgtggct ggcgatacta
    31981 tgtatgaatt tttctcaaga agtagacaac aagaagtttg taatgcaata atgtggtatg
    32041 ttatggaagc ttgtaacact gattgtgtac ttgctcctga atttcaagaa ggtggatttt
    32101 ataaagtaaa ggatgcttta gaaatgttat cacatcataa agagcaagct ttagtagaag
    32161 tatcttataa aaagtttaag gaattaaaga aattagtttc agagggcgat gatgttccag
    32221 caaattaatt gtgaatttag aggattctga ggaagggata gccccttcct tttttaattg
    32281 aagcagtttt aagcgtaaat tatatagtct ctttaaacta tgatttatca atttagaaat
    32341 ttagtagttt atatttaaaa tcagtaagat atatttaaaa taaaaattaa aaagtataca
    32401 attttattat ataattgtct tatgatgttg attaaagaat tattaaaagt ccatttattt
    32461 ttaaatagaa taattttata aatatatatt tgatgtgtat attagaaaat gattaaaata
    32521 ggaggaaata caatgagcaa ttacaggaca ttacatgaat ttggaacgga tgaaataacg
    32581 attgaaaagt ctgtttttat tggatatgct aagcctataa agagtgaaga agaagcatta
    32641 gagtttataa atgaaataaa gaaaaagcat aaagatgcaa ctcacaatgt gtgggcttat
    32701 actgtaggta aaaatatgaa tatacaaaga tatagtgatg atggagaacc acaaggaaca
    32761 gctgggatac ctacattaga ggttataaaa aaagaagatt tgagagatgt agcggttgtt
    32821 gttactaggt attttggagg aacaaaatta ggtgcaggtg gtctagttag agcttataca
    32881 aaaggtgcta aactgggact tgaagcaggt aagataattt ataaggtaat gtatcaagaa
    32941 gttaaggtta agatagatta tactcaactt ggaaaagttc aaaatgagtt aatgaatttg
    33001 gggtatttta taaaagatac tgtatatgag gataatgttg aaatagtagt atattcaaga
    33061 ttagaagatg tagagaaatt atcagaaaaa atgattgata taacaagtgg aacaggaaaa
    33121 atagttttag gagaggaatt ttatttgtct gaacaaaatg gagaaatttt aatataatct
    33181 aaaaggttat tagaatagtt aatacctcaa atagccaatt agtattttta agtgaaaagc
    33241 cattaaattg atttaatcta aatcaattta atggcttttt atcggatttt gcacagaatc
    33301 ctttttttac ttcaaataaa ccgactaaaa gtaaaaatat cccaaatatt ttttttaata
    33361 tatctgaaga cagataatta gctaatagtg aaccaaatac agctcctaaa accccaaaca
    33421 cagctatagg tataaccaat ttaaaaatta catttttatt ttttatatgt atagcaagtg
    33481 ctataagtgt catagggata gatgaaagta aatttacgct ttgtgctatt ttagtgtcta
    33541 tgccagcaag taaaacgagt gcaggtataa ggattgttcc tccacccatt cccattcctc
    33601 caataattcc tgcaaaaaat ccaattagag caaataacat taaaacacca tccttaatgc
    33661 tgcaattatc ataattattc caaaagaaat tctaagaaac tttccagtaa ccttatttag
    33721 taatttagct cctataatac caccaagaat acttccaact gcaactttaa ttgttaagtt
    33781 aacatcataa gtacctttag atacatataa aacagaactt gctgtcgtta agaatattat
    33841 tatagaaaga gcagttgcat gagatttatg ttcttctacc ttaacgatgt catttaatat
    33901 tggaactaaa agagtacctc ctccagaacc aaaaactcca tttataaatc ctgtaaaaac
    33961 tccaattatc gtatttttta agttaaaatc aaagtatttt ttctttaaat catcagtttt
    34021 tttcataaaa atcaccttct gaaatagttt taccaaaata ggcaaattat atttatataa
    34081 aataagatta aatataatct ttcatgtttg tttaattttg cataaaaaaa aacatattaa
    34141 aacaacatat taatatatgg tataatatat taaaacaaag tagcaaattt gagaggagat
    34201 aacaatgtca aatataaaaa tacaaataga caaaactgta gaatggctta taaataaggt
    34261 taatgaggca aatgcaaaag gattaattgt tggtgtatct ggaggaattg attcggcagt
    34321 agttgctaat ttaattaaaa aggcatttcc agaaaactct atgggagtta taatgagcat
    34381 aaaaagtaat cctcaagata gagaagatgc acttaaggtt atagagggat gtgatataga
    34441 ataccttgat ttagacctta tagaaccaca aagtgctata ttagacatgg tagtaggaaa
    34501 tttaaaagat aaacatttat atagagaaga atatttaaag atgacagatg caaacttaag
    34561 agcaagagta agaatgagta caatttatac aattgctaac aatctaggat atctggttgt
    34621 tggtacagac aatgctgcag aaatacacac aggatatttt actaaatttg gtgatggtgg
    34681 agttgatatt ttacctattg caaatttaac taaaggagag gtgtatgaat gggcaaaaga
    34741 gcttggtgtt catgaagatt taataaacaa agctccttca gcaggtcttt gggaaggtca
    34801 aacagatgaa gatgaaatgg gaactactta caatatgata gatgctgtgt tagaaggtag
    34861 attggatgaa gttcctaaaa gagaccaaga gataattgaa agacttcata gattaagtga
    34921 acacaagaga aaaacgcctg cacaacctcc taagttctaa tttactattt ataagatttg
    34981 tgataaaatt tacaagtgat gaaaattaga ataattaatt gtacgtatta aggagtggaa
    35041 atatgggacg tataggtaat ataattaata gaaaaggtaa acaagatgct caaagagcaa
    35101 agatatttac taaacatgct agagcaatag cagttgcagc taaagaaggt ggagcagacc
    35161 cagagtacaa tgcagcgtta aaaacagcta tagaaaaagc taaggcagat aatatgccaa
    35221 atgataatat agatagagct atcgctaaag gtgctggagc aggtgctgga gaagactatg
    35281 aaactatagt ttatgaagga tatggacctg gtggagttgc tgttatagta gaaactttaa
    35341 ctgacaacaa aaacagaact gctggtaacg taagatacta ttttgacaaa aacggaggaa
    35401 acttaggaac tagtggatgt gtgtctttca tgtttgataa aaaaggacaa atattagttg
    35461 gtttaggaga tggagtttca gaagaagaat tgatggatgt tgcattagaa gcaggagctg
    35521 aagatttcat aacagaagaa gatggttatg aaataataac tacaccagaa gatttttcta
    35581 gtgttcgtga tgagttaaaa gctaaaggat atgagtttat atctgctgat gtgaagatga
    35641 tacctcaaac tacaactgta ttgactgaag aatctcattt aaaaatgatg aataaattag
    35701 ttgatatgct tgaagaagat gatgatgttc aagatatata tcataactgg gaagtagaat
    35761 aaatagtata agcatattga aatttataag tattgaatca attttaacat gtgataaatt
    35821 ttatagaata agaaacacta aaatagacat acgcacctct aacctatatt tgcataataa
    35881 tatgtaagca taggtaaggg gtgtttttat atttataggt taagaaattt aaagtaaatt
    35941 ttgagctgga ctcgattgtc tataaataat tgatgggtat ataacttgtt gctgaatacc
    36001 atttatacgc ccagttactg tatcaataat catcctaaga ctacgttctg ctgtctcttc
    36061 catttttaaa tctataactg tcaaaggagg aatacttctt gccattagtc gtgaaggtcc
    36121 attacttgct gcaaatattt gaatatcctc tccaattaca attcccgatt catgaaatgc
    36181 attcatcata ccaagagcta ccacatcatg attacaaaaa attacttttt ttagtgaatt
    36241 ttttgcaagc attttttgag caatatcata tccactatct attttattag aacaataata
    36301 tatagaatct gatagatgta ttccatgttc tttacatgtt tttaaaaaag atttacccct
    36361 taaagtcata catgtaaagg aagttgggtt gaatacaaca gcaatatcat ttttaccatt
    36421 ttgaatagca tgtaaagctg ctatacgacc agtttcctca ttatcaatag atacacttga
    36481 aaaaccaggt atttcacgat ttacaacaac tacaggaatt ttaggtttat tttttactag
    36541 atattctata tcatcagcat tgatacctgt taaaacaatt ccatcataat aattatttgc
    36601 aaaaaagtat tcatcatcac atagatgacc aactttgtaa ggtcgtatga tgatatttat
    36661 gtttaaatgt tctaaagaaa ttatgtggtt tattgcttga agtatattgg ttgatgccaa
    36721 ctcaaatccc tcatcctgcc aataaaaagc tattttaata ttattatgtt taataggagt
    36781 agagcgttta tatccaagct tatcagctga atttaatata agctcttgtg tatgtagcgg
    36841 aatcctgcgt tcatttcctt taccacttaa gacgtacgag acagttgctg aagaaagacc
    36901 acagtctttt gcaatatctt tgattgttgc catgataaaa tcctccttaa atagttgctt
    36961 aaatttgctt ataccaataa tcttaacata tattcaacta aagaacaata ttctacaagt
    37021 tgcatatttt acaggattag aataaataaa tttaaaaaaa cttcttactt atgttgacat
    37081 aatttagttt ataggattat aatatgctta aataagctta tgattaactt aaattaagca
    37141 aagttaagca ggaacatgca atgctagctt gcaatgtgac tgtaaagggg gaaaaggtat
    37201 gagtaaggta aatatatttg aagttggtcc tagagatgga tttcaaaacc tgaaagagtt
    37261 tttagaaact gataaaaagc ttaagattat tgatggtctt attgcttctg ggattaaaca
    37321 acttgaaatt agttcatttg taagcccaaa agcaatacct cagatgaaag atgcaaaaga
    37381 agtagcaact tattgtgtag aaaaatatcc tgatgtgaaa ctttatgcat tagttccaaa
    37441 tttatacggt gctaaagttg catgggagtg tggtataaga gatatatcct atgttatatc
    37501 tttgagtgaa actcataata aaaaaaatat aaatcgtacc catgaagagt ctattgatga
    37561 gcttaaaaaa ataatgaata cttatccaga catgaatata tgtattggaa tggctactgt
    37621 atttggatgt ccttttgaag gtttaccaaa aattgaagat gccataagat ttgctaaacg
    37681 cctttgggaa aacggtatca gggagataaa tctagcagat acaataggga ttgctgaccc
    37741 taagcaagta agagaaacta ttagtgcatt agttgaagct ttaccagaat gcagatggca
    37801 ggtacatatt catgatacta gaaatatggg aatggttaat actcttgtgg ctattgaatc
    37861 tggaattact actgttcagt ctacacttgg aggattagga ggatgcccat ttgctcctgg
    37921 agcatctggc aatactgcaa ctgaagattt agtatatatg cttgaacgta tggggtatga
    37981 tacaggagtt gattttgtta aacttcttga attatcaaag tatcagaaga gttttgtaaa
    38041 tggagtatat agtggtcatc atgtaaatat aaagtctgat agttgtaatt gttagttcta
    38101 ttgattaatc gattaaaaaa atatgttgat taaaaaaata tgctgattgg taaaatagat
    38161 ttattgataa aaaatataaa ttataaaata cattatctaa taaaacaaat aaaaaaattg
    38221 agggggatta gtatggatat tatagcttta cttttatttt taggagttat tattattgca
    38281 tttgtacgta agaataatgc aggtattttg gctctagcta ttggtgtcat agctgttaga
    38341 atttttggaa tggatgataa agaactttat ggagctataa gcaactcaat gtttactaca
    38401 cttgttggta taacactact ctttgcaata gtgaactcaa caggagcatt aaagcttgtt
    38461 gcagaaaaaa ttgtagctgc ttctggtaaa cgtgtttggc ttatacctat agcagttttt
    38521 atagcaggat ttgttgttgc tggtgttgga cctggggcaa ttccagcact tgcaattatc
    38581 ccagcaatgg ctgttcctgt agccataaca gttggttata accctgtaat gcttgcactt
    38641 ataggtgaat gtggattaat ggcaggtcgt atgactccta ttactccaga aggtcaaatt
    38701 ataacaaccg ttgcagaagg ggttggaatt gaaaatgtta tgcctactat cttagcctgt
    38761 caaactcttt ctacaattgt attttcaata gtattgttta taattttcaa aggatataaa
    38821 cttaaaaaac ctataaatgt tatagatagt aagcaaatag aaaaattcaa tataaagcag
    38881 ataatttctc taataagcat agtagttatg attgctttta tcataatttt tgatatgaat
    38941 gttgggcttg cagcttttgc agtttcagct gtacttattt ttttcaatat tgctgatgaa
    39001 ggagaatgta tcaaatcaat tccatggagc acgatagtaa tggttcttgg agtaggtgca
    39061 atgatgacaa tcgtagatgc agcaggaggt attgacttga tgagtaatgc attatcatca
    39121 ctgatgaata gtaaaacagc tactccaatt atgagtattt cagcaggact tatgtctatg
    39181 gttagttctg ctttagcagt agtttatcca actatgatgc ctatgtgtgt tgatatagct
    39241 aaggcagttg gaggagtaga cccactagca cttattgctg cagttggtgt tggaggttct
    39301 cttgctggtg taagcccact ttctactggt ggggcattga ttttagcagc tatgggttca
    39361 agtaagaaag actttaataa ggaagaacaa aataaggttt ttgtacagct tttcattatg
    39421 agtgcagttg gcttattggt tattgcagta gtatcaatat tatcatttaa tgcaataaca
    39481 catttaatga actaattaga ataactgaaa ataaatcatt aaatttaact tttaatatta
    39541 atcaatataa tataatttgg aggggaagtt atgaaaaaag gagcattaag caatataaag
    39601 gtacttgacc ttacaagagt tcttgcaggt ccatattgta caatgatgtt ggcggatttt
    39661 ggagctgatg ttataaaaat agagatgcca agaaaaggtg atgatacaag aggttttgga
    39721 ccttttgtaa atggagagag tttatactat gcaaatataa atagaaataa aaaaggtatc
    39781 actctaaacc ttaaatcaga agaaggaaaa aatatatttt tagaaatggt aaaacatgct
    39841 gatgttgtag tagaaaatta cagaccaggt gtaatggaaa agttaggtct gggttatgat
    39901 gagttggcta aagttaacaa tcaaataatt tatggagctg tatcaggatt tggatgttat
    39961 ggaccatatt cagaacgtcc aggttatgat attattgcac aggcaatggg tggtcttatg
    40021 agtataacag ggcaagaagg aggggaacca acgagagttg gtaatgctat gggtgatgta
    40081 ctaggtggaa tgaatttaac tattggtata ctcacagcac tcaacgctag aaaaataata
    40141 ggaaaaggac aaagagtaga tgtttccctc gttgattctg ttgtatcgtc tctcgaaact
    40201 ggtatacaga gatactttga ttcaggacaa cctgaactta tgggtaatag atatgcttca
    40261 gcctatcctt atgactcttt taaggcaagt gatggaagtt ttgtaatagg ctgtggtaat
    40321 gacaaacttt ttgaaaagtt atgtaaaaaa gttcttttta gagaagacct tttaatagat
    40381 gagagattta aaacaaatct taatcgttgt gaacattaca aggctcttaa accagaaata
    40441 gaaaaatggt ctgttaagca tacaataaaa gaagcagttg acttaatatc agaggctgga
    40501 gttcctgctg ctcctatcta taatcttgat tgtgttacaa cagatgaaca tatagcaaaa
    40561 gcaagagaaa tgattgtaga tattagtcat cctattatag gaaatatgaa agtcaatgga
    40621 aatccagtta agttgatgac aacaaaagct aaaattgata tgccagcacc atctctaggg
    40681 gaaagtaacg aggagattta taatggttgg cttggttttg atagtgataa aataaaagat
    40741 ttaaaagaaa atggagttat ttgattaaaa aatagctaaa cacacttgtg actttagtca
    40801 tgggaggttc agttagaata atatcaaaaa atattaaatc aatactgtaa atcaaatata
    40861 gaattgtcag aagcatttaa gatgggaaaa gctacctaga cattgagtct ttgtggcttt
    40921 tcctattttc attaagaatt ttatattatc agaatagata tgtactaaac taaagtatta
    40981 acaatttaaa ttaatagaaa ataaactacc taattatgtt gaataaagac tactttagtg
    41041 aaaacaaact tatagagatg ctatttaaat tatt
//
LOCUS       DBGJFF010000023        41037 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.023, whole genome shotgun sequence.
ACCESSION   DBGJFF010000023 DBGJFF010000000
VERSION     DBGJFF010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 41037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41037
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.023"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            <1..1099
                     /locus_tag="ACOIAF_001227"
     CDS             <1..1099
                     /locus_tag="ACOIAF_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="endopeptidase"
                     /protein_id="HGZ2668246.1"
                     /translation="KKKYYSIEVGDLINHLDFLNNKFNNIENKIDTVKKAIDNVKVDD
                     SEFPNTLPEVPILTAEGLFAFINLTWTFTNKSYYKYEVYASQIKDFVPDTTNFTNRIF
                     AGQASAYVHQAEPFQTWYFRVRAVNSHGNVTEFSNQVEAQTTKMSDGATWIQEGAIAD
                     ALIGQLKLDRGWFGQLKGSYINARELVVENDNGVKTMEVDSFGDVHFNPNTFKVSFNE
                     ISPHVLIDDEGLKLISGKGFTRMTESGLYTKFYTGSDVTESWYLQESGYTYFDYKDSK
                     IIIDLPKKFAGKNFKATCAIRSTSTNASCLSYFSTSATVKKSDTNPQLELSASVRGVS
                     YEMSGSQTIGYWLGMNFFSEGFITVQYYVYA"
     gene            1124..1366
                     /locus_tag="ACOIAF_001228"
     CDS             1124..1366
                     /locus_tag="ACOIAF_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668247.1"
                     /translation="MVTIFYSKRTGEIYNCIKSEVEQDYTCFADREEDYRQILDKIVV
                     EDIPEILNPRDYKIENGKFVLKEKEIIKEFTPKMVE"
     gene            1381..3615
                     /locus_tag="ACOIAF_001229"
     CDS             1381..3615
                     /locus_tag="ACOIAF_001229"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668248.1"
                     /translation="MRDRIYTVDINTKSYQVAKYKQYDNGIEFKINLLENNIEKDLTG
                     YTAIANFQRPDRKIVYQSCTIENSIVTTLIENNITAVAGDVIVEFTFYKDDLVVTTFS
                     LKINIEKSIDKNSITEEPKWDYISVTINQVKEVVEGIEEIKETEEARKEAEIERVKEF
                     NSIKETFDSKVTEVTDAKNTMISDVNTTKDTLTSEVTDAKTDLISVVTTAKENMINEV
                     SAVKTELETAEAERVEEFNSIKETFDSKVTEVTDAKNTMISDVNTTKDTLTSEVNTVK
                     AEVITAKENMISEVTTAKETMQTEVTDAINAIPTKEELKGVGIEIKGSLDNISALPVN
                     PTLSDAYFVKSATIENQIDLYVWDNTNWVKVPDIKIKGENGDGLEFNWDGTRLGVRIE
                     GQENYTYTDLKGQKGDKGDSIEFNWNGTRLGIKIEGQENYSYTELKGEQGYTPTIGEN
                     GNWWINNIDTQKPARGASLRILGKLDSIDNLPLDPTIGDCWIIGRNIYIYQTKWEDLG
                     SLAGVDGKNLEFNWDGTQLGVRQQYELDYKYIDLKGDNIEFAWDGTRLGVRIEGQENY
                     TYTDLKGQKGDSIEFSWDGTELGVRIEGQEDYSYTNLKGATGNKLEFNWNGSQLGIRE
                     EGQTEYIYTELRGEQGYTPAIGENGNWFINGEDTGKASKGKVTWNELLEKPKELDYIK
                     KSTTFNSDGSITDILDSVSKTITKFNADGTIVDEKYIDNVLVSKVKTTFKGNQIEEIK
                     EEVS"
     gene            3615..5498
                     /locus_tag="ACOIAF_001230"
     CDS             3615..5498
                     /locus_tag="ACOIAF_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016040100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGZ2668249.1"
                     /translation="MSWAETYKVNSDLQGEPLNFLSYLQDIKLNGLDSYVLFIGNARI
                     WEELYLNSLYLFSDRGIRETVYTAFSETDIDNLFNKSTKLGEQLNAFYRTDIFSLGNA
                     DNVVKEMTIEHYNSLEEKFKAGYDRYVTREQEKSTIGAWFNSTFSLNNTGLESLTTIE
                     EILANVEATNAILNNSNAIVALTMCKTSMDAVAASPNAMDLLGQYILKVTAEPAVIRA
                     IIKNEVIRDAIINSDEAMTQISSNENSVMEIFNDLEATKVLVQNQNSINKILTNNVTV
                     EKIIPNLLEMKYNLQTSLNYINTIKSNIASGKGQIMAITYNEEIFPILKNAAKNYDGM
                     ENTRNILQRDIEEKIKISDAILESSIAMTTFANNSIIVNKVGDRAGIIESILGQTVPL
                     NAFMKSTTAIKVLVNKATAFTKIVNNSTAFNAMLTISGNISSVANSSTAMNIIANNAQ
                     VMATVARNDTVLTVFINASTAITAIAGNVTSMSAIVGYSGAMNKITTSAFALNRIFKS
                     TIGKDILISNNAILQTYKNNIHNTLRSSSGQFRLITSPTDSDSAPTQIINSSYVGVTY
                     CYGYRGGTGKFAIVYHGHKTSAEAGRGDGGKDETKKFITLGGTKFVESGDGYFNYSIY
                     QAI"
     gene            5585..5848
                     /locus_tag="ACOIAF_001231"
     CDS             5585..5848
                     /locus_tag="ACOIAF_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668250.1"
                     /translation="MEQLLTELSSLGAIGILCALLFKNTMQEKKEDRDMYKKTVENFI
                     ELSTQQQEINKNILVEMGAMKTDVEEIKEDVTDIKDMLQKESL"
     gene            5849..6655
                     /locus_tag="ACOIAF_001232"
     CDS             5849..6655
                     /locus_tag="ACOIAF_001232"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HGZ2668251.1"
                     /translation="MKVALTAGHTLTGKGTGATGYINEGTENRILMDLVAKWLKKGGA
                     TVYSGKVDKSNNYLAEQCQIANKQNVDVAVQIHFNADHTTLDKMGTETIYKTNNGKVY
                     AERVNEKLATIFKNRGAKSDARGLYWLSHTKAPAILIEVCFVDSKADTDYYIRHKDIV
                     AKLIAEGILNKTIDNKENSEDKKMYKHTIVYDGEVDKIPATVVGWGYNDGKILICDIK
                     DYVPGQSENLYVVGGGACEKIGSITKEKYTMIKGNDRFDTLYKALDFIDR"
     gene            complement(6724..7161)
                     /locus_tag="ACOIAF_001233"
     CDS             complement(6724..7161)
                     /locus_tag="ACOIAF_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668252.1"
                     /translation="MNAKLNKELYNLGFKIGSENVLSRNKILELNDAIYRYQDRNKAF
                     EIILKAFMKLDIPMPAEIIENLDNYEVIVNFVVGVYNGYIEQLNNFSNFISLSDASKK
                     YNKAESTLKQNIKNGKFVEGVDCRLFGKSWVFNIDSLEREYSK"
     gene            7333..7629
                     /locus_tag="ACOIAF_001234"
     CDS             7333..7629
                     /locus_tag="ACOIAF_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668253.1"
                     /translation="MKKITKKEIIEFVRDVVEEYRDWKLEKCGFYIKDNELNSFVSFE
                     GKGIDINVYKENYDEIIYIEDYIKDYKRKEYNLKEIDSIIYEDVNEMINNYNEK"
     gene            7669..8139
                     /locus_tag="ACOIAF_001235"
     CDS             7669..8139
                     /locus_tag="ACOIAF_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668254.1"
                     /translation="MNKQKARRFLRVIDMNMDKIEEEAIKAFKESCLIKEENDIKIFI
                     DFQGKVEAIAIQAYTKLLDDNKEINIFTSRQIENNLDDMFGEICYVNDYEEFVNWCED
                     KCENLNWDSYKKFNKENFEEIAERNIDDSTPVFLEELREGIESCKQELQNIVEN"
     gene            complement(8375..8656)
                     /locus_tag="ACOIAF_001236"
     CDS             complement(8375..8656)
                     /locus_tag="ACOIAF_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668255.1"
                     /translation="MNVYLQIGKDLSEKMTKSELNKFSQEVVNFLRYAGVDKDMKIKL
                     FQTIIEAVARKKITLPNDNKFTEGILQSSEFEMTENVGFILEGLSYRNN"
     gene            complement(8801..9133)
                     /locus_tag="ACOIAF_001237"
     CDS             complement(8801..9133)
                     /locus_tag="ACOIAF_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016040102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668256.1"
                     /translation="MPSFNRDLLVDKEKPILKNGTIVQNDSTTTDNKTLNIYNLVNKK
                     SKKVTMSATLDEELVNKLKSFSIDMNSDVSKLLSDILTQILSDVTIKEENLNIYNERN
                     RRNKTKKK"
     gene            complement(9134..9943)
                     /locus_tag="ACOIAF_001238"
     CDS             complement(9134..9943)
                     /locus_tag="ACOIAF_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HGZ2668257.1"
                     /translation="MANTKLLTYFNIKGGIYKTTTSIMTAYELAKDKDKKILLWDLDV
                     QANLTQYVYEINHNDNTTLDILKGISANDAIVKSPNENYINVDLIPSDIQMARFEQEL
                     SPLPAREKFLARWYMQNFNTLSEYDYIICDLSPRYDLTAKNVLFLADSIIIPIQDKNI
                     SSLRGAELFKQLWDVDRTYFDKEDNIKSTILVGFEKKKTQISDTFDSYLEGFNDMRDI
                     MLDTYIRKNEFIEKALLKKLSLTDYTKITKEHFSRQEFTNMLEELKRKGVL"
     gene            complement(10254..10436)
                     /locus_tag="ACOIAF_001239"
     CDS             complement(10254..10436)
                     /locus_tag="ACOIAF_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HGZ2668258.1"
                     /translation="MEKEKRDLKFNFHKGGSGSFTPRMSVPKKWADEMGITRENPNVT
                     ATFEGDRIIIEKAKNE"
     gene            complement(10676..10804)
                     /locus_tag="ACOIAF_001240"
     CDS             complement(10676..10804)
                     /locus_tag="ACOIAF_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668259.1"
                     /translation="MGRLERSKKKRENKFNIVKKVFSFILLLLNIILAILRILKEL"
     gene            complement(11257..11409)
                     /locus_tag="ACOIAF_001241"
     CDS             complement(11257..11409)
                     /locus_tag="ACOIAF_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668260.1"
                     /translation="MDNFLLNVLAGVIASLIFYIISKLFRQVKNHSAQKSGWEFDLRI
                     KFKKFK"
     gene            11925..14153
                     /locus_tag="ACOIAF_001242"
     CDS             11925..14153
                     /locus_tag="ACOIAF_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HGZ2668261.1"
                     /translation="MKNVNKNNIDKLFKNDCDLDRAVGEIENYAKFERQETHKIKKYI
                     DEKKSGVYNAIARAGAGETVLLLAETGTGKSYSFINALKKMNTKALFILPNSANVEQA
                     MCEYDIPGAYDLMPAPKALKEGNLSVFTWDKTEQLLNIDLSDYIIVVDEIHQTYTDSY
                     REKAIKNLNNIVAKCKGRIDITATPTKLEFEIYDYIIEYIKEKNTKYDVKLYDNINLD
                     KMIEIINKSKNSAVLYNNKKDLKYINELTTKKGDIVYAKIKAKSELYKRIIQHSDMGD
                     YELLLNTTTILAGVNIKNKDITDIIIAGFKDPATIKQYVARFRELKRVRVHIFNNFND
                     TEASNKVYEVNNIININFKRANKLKALYNELNSKRATGFETVGIDLVPFSIDSNVYYS
                     TKNKCYEIDKLFIISNTYTRYYNSRTIASLKVLLEEYFKDVKIENIEEQDEETKQGIK
                     EYKKVLKEIKTATLKTLEENKKYLVGYEQIKRGSKDFNLMSYMNYNKMSENDYKQAYI
                     KYNIDYLVDKADCYSEIELFSKYVLEHNYSLELSWKLANMGNNKRGNIFKKINNLIYR
                     ELKEEYPNLINDNLIEVVAYNYIDKLFGIGTAYTEYNLQELSNDLAIILGDNWRFATK
                     KLGNVLKNIFIVKAKQHRNFEALENYSYYKNNKPNTDFIDSDRIRIKKIERYITIEDI
                     EKDLKLEKGDLTLRNAIKIRKKKLLDNLDEIEKAFLNKELDKELRIEREIELVEQLEI
                     CI"
     gene            14400..14981
                     /locus_tag="ACOIAF_001243"
     CDS             14400..14981
                     /locus_tag="ACOIAF_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668262.1"
                     /translation="MKKILLCVIIGMLGLTLVACSSTASGDKIDGIEIVAKETSAEQI
                     KGDCNQDGSYNENGEYFQMGLDTAKASDYEYKIVNVSVKNASDEAIKLFQTGWNATAK
                     DGYEFEHIRVTDKLENQQVPANYSFDAQVKILVEKKMNVKEIILKYNLKDYSNLTNAM
                     KDASSGATKEDIQKKYPELYDDNWIEFGTIKFE"
     gene            15238..15381
                     /locus_tag="ACOIAF_001244"
     CDS             15238..15381
                     /locus_tag="ACOIAF_001244"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668263.1"
                     /translation="MKIEYINTEEYTEFIEFIEFIVSAKKTKMKEFIYIKKVLFELEK
                     EDR"
     gene            complement(15399..15767)
                     /locus_tag="ACOIAF_001245"
     CDS             complement(15399..15767)
                     /locus_tag="ACOIAF_001245"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGZ2668264.1"
                     /translation="MKERNSNEGKIPGICERVAEIRSFENISQKAFGEKINLSQSQIA
                     SYEKGYRIPTDRAIDDICRVYKVNKDWLVNGIGKKFIDPLIDLDLDPEIEEMAKLYLK
                     LTPSKREHIKKLMQKSLDSE"
     gene            15940..16092
                     /locus_tag="ACOIAF_001246"
     CDS             15940..16092
                     /locus_tag="ACOIAF_001246"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668265.1"
                     /translation="MEYNIPYDQKDFLEFLKLGETIMGQNPLKFQRLIGRMQGIVDCL
                     EDEEEQ"
     gene            16174..16599
                     /locus_tag="ACOIAF_001247"
     CDS             16174..16599
                     /locus_tag="ACOIAF_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668266.1"
                     /translation="MIKVILCSTIDEYMEYEDVIGAKDSYLMDVIIGGECSEIDAIET
                     LEILKNSKARIVSEAEKTCLKFGIGNQISKAIEQGIKEAPKLKKGIHCDNCGASVGSS
                     EELTSMPVLENYNNNQSVSDQLGSIDVCPSCLLEITDLL"
     gene            16678..17082
                     /locus_tag="ACOIAF_001248"
     CDS             16678..17082
                     /locus_tag="ACOIAF_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HGZ2668267.1"
                     /translation="MINLETLCNGETKEKIENGFMEIFKNIQDPNTPATSTRSLTVKV
                     TLKPGKNRSHVNTQIQVIPKLASVLPSETDIIVEKDFRTGEVNANEYGNQLPGQVKLG
                     DLESKETSVTEETEVKESEEDNIRKFKSLKDL"
     gene            17105..17818
                     /locus_tag="ACOIAF_001249"
     CDS             17105..17818
                     /locus_tag="ACOIAF_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668268.1"
                     /translation="MLTDLFNRVMEVGARKIETREINGVTYTTDRLIPVYPKTVEEIE
                     LNTLDGLIEYIKSEVDKDAKGNRLIVQIKSPKSVRLCSELLEGGKRNTYVDCKALLPH
                     INFDCSLDSEEFNIMLQSSFVDTEDKNLLLKVAGNVKETSVKTVQDDGVSQVVAMSTG
                     VASVEDVILPNRVSLKPYRTFTEVDQPESEFIFRVKEGITFKLIEADGGAWRLEAIKN
                     IKEYLEKKLEGIENIDIIA"
     gene            17876..18040
                     /locus_tag="ACOIAF_001250"
     CDS             17876..18040
                     /locus_tag="ACOIAF_001250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668269.1"
                     /translation="MYKDITKMGAIERGEYVGNVALELREENPHLSKIDLIELALKKI
                     REESEQEAGE"
     gene            18037..18348
                     /locus_tag="ACOIAF_001251"
     CDS             18037..18348
                     /locus_tag="ACOIAF_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668270.1"
                     /translation="MKIGKYLIIPQEQEEARIEDLKNEVRKRDDRIEFLVYVGNKNRE
                     LIKNLEEQLKGYKSELDVYSLFIDCILNKETRKAIAIYNRTKSIRIKQKCLNSIPIKK
                     G"
     gene            18394..18543
                     /locus_tag="ACOIAF_001252"
     CDS             18394..18543
                     /locus_tag="ACOIAF_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668271.1"
                     /translation="MSEFVQCCCCERTINIEENNYVQYEKEALGLVFTLYFCLNCVDE
                     LSEME"
     gene            18574..18747
                     /locus_tag="ACOIAF_001253"
     CDS             18574..18747
                     /locus_tag="ACOIAF_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3797 domain-containing protein"
                     /protein_id="HGZ2668272.1"
                     /translation="MIMKNVLKLLAMYCFCPECGSDELGEGEGSLIVDEYTFHRKCKC
                     GFDVIVDEREDKI"
     gene            18859..19263
                     /locus_tag="ACOIAF_001254"
     CDS             18859..19263
                     /locus_tag="ACOIAF_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HGZ2668273.1"
                     /translation="MNNTTLIGRLTRDPELKYIPGSGTAVSTFTIAVDRDYIKKDGTK
                     ETDFIPIEVMGKLAEVCANNLDKGRLVAVEGSIRVEHYEKDGEKRTYTKVHANKIKFL
                     DYKKDDTGKAYMFEPGGLDPQGFQAIDDGIPF"
     gene            19282..19671
                     /locus_tag="ACOIAF_001255"
     CDS             19282..19671
                     /locus_tag="ACOIAF_001255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668274.1"
                     /translation="MNEKWKQYIENVLESRGYCINRMPYVYSYDFLVNSNIKINVEIS
                     NLYKNEENEYHYFELEKEYHSCDIYILIALDEVGSALKILVIPASNLMGQKQIIINNK
                     SKYDKFDSRFELIKVYDNFYNDLDCVM"
     gene            19683..20045
                     /locus_tag="ACOIAF_001256"
     CDS             19683..20045
                     /locus_tag="ACOIAF_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668275.1"
                     /translation="MFKKVEKKFSGSVNPNFMEDDLTFKNYLALYCCIKPVKIHGNKK
                     VLASSTKACSALKIREHQKKARKKVKLINAKTGEIKEMAIDEAENFLNVKNLYAVIWR
                     GRPTRTGWYVKDVGNEGD"
     gene            20032..20202
                     /locus_tag="ACOIAF_001257"
     CDS             20032..20202
                     /locus_tag="ACOIAF_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668276.1"
                     /translation="MKEIKYNVIYFNSEALKMDKRSFDALKDARAFKKEKEKKYKNVE
                     IIKKTIIEKLIM"
     gene            20214..20786
                     /locus_tag="ACOIAF_001258"
     CDS             20214..20786
                     /locus_tag="ACOIAF_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HGZ2668277.1"
                     /translation="MAKKKRICSWCGKSFFTESKNRFCCKSCERKYKKDKEKNASSCL
                     VEETKEYLIELGNKKRRVDILRKEIDFLKDKEFYREINFNELGFKIHSSPKGIDEMIM
                     NNEDKISFKESEIDFIEYRLRLMDIYISELTEEKQQIIKFMYFYDGASKLSNEEIASK
                     IKCSRSNVHYKHKEALKELAEMLFESGSLL"
     gene            20965..21573
                     /locus_tag="ACOIAF_001259"
     CDS             20965..21573
                     /locus_tag="ACOIAF_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGZ2668278.1"
                     /translation="MGSKKPANPVKDKRMVLNIQEYLKEKNIRDYVLFVLGIGTGYRA
                     GDLVKLQVRDVRNAIDEGYFLIMESKKEKTKNIRKKNKKPRKAPIVPNLERVLKSYIR
                     DKKDYEYMFPSRQKSVTPYIGVERVTVILKEAGRYFGLKHITAHSMRKTYAYTIYEES
                     GFDIIRVKEMLGHSSIEETKAYLGLNEEQYQEYSMFLNDLIG"
     gene            22038..22607
                     /locus_tag="ACOIAF_001260"
     CDS             22038..22607
                     /locus_tag="ACOIAF_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HGZ2668279.1"
                     /translation="MIFGYCRVSSKVQIDNNSLEQQEQEIKRHYSDAKIFREQHTGST
                     THRPIFEEMVNQMRDGDKLVVTKLDRLARNATEGINLIQNLFDSGISVHVLNVGLLEN
                     TTLGKFFITTLLAVAEMERNLILERTFAGKEIAKQNPNFREGRPKKYTKMQMEHALEL
                     LKTNSYTQVEKMTGISKSTLQRAKRKQED"
     gene            22636..23343
                     /gene="terS"
                     /locus_tag="ACOIAF_001261"
     CDS             22636..23343
                     /gene="terS"
                     /locus_tag="ACOIAF_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HGZ2668280.1"
                     /translation="MICISIKNSNREKAFEIYKEKNGRIKLIDISKMLEEKSCNISRW
                     KKIDRWDYRLGINKKVGAPAGNQNALGHEGGAPAGNQNARTHGFFSKLLPARTYEIVK
                     YIEKDGGNSLDILWNSIVVQYAKILDSFKITHVKNKKDHTTDIKKNGNKIKEYEIQHS
                     WDKVTNAMKAEATAFKALSKMIKDYEELLHKNWDLATEEQKSRIENIKARTNKLTGND
                     LEIEDIEEIEAEIYGSN"
     gene            23330..24652
                     /locus_tag="ACOIAF_001262"
     CDS             23330..24652
                     /locus_tag="ACOIAF_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HGZ2668281.1"
                     /translation="MAVTKKKTIPFQFGDKHKEYIKKCAINTYNIAEGAVRAGKTVDN
                     VFAFAHEIKTSKDKIHLATGSTSANAKLNIGDANGFGLEYIFRGQCHWGKFKGNECLY
                     VKGISTKHKQKIVIFAGGAKADSYKKIRGNSYGMWIATEINLHHDNTIKEAFNRIIAS
                     TNRKIFWDLNPDNPNSTIYKEYIDSYMKKDKEGTLLGGYNYQHFTIHDNITVSEERKQ
                     AIISQYDINSIWYKRDILGQRCVAEGLIYEYFANNKEQFKTETVDPLMDVIIGVDFGG
                     NKSYHAFVATGITYNYKKVIALASERPNADTSPNELNALLINFIKKVINLHGKVDYIY
                     CDSAEQVLIKGIKNAVEKEKLNVSVRNALKSAINDRIRLTDLLISQGRFEYTKHSETL
                     VNALCSAVWDSKETNEDVRLDDGSSDIDSLDAFEYTIERYLKKFIRGD"
     gene            24653..26065
                     /locus_tag="ACOIAF_001263"
     CDS             24653..26065
                     /locus_tag="ACOIAF_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGZ2668282.1"
                     /translation="MFEGLKNAIRGVINKLFNKSSIQNELKVDIAVSDKMSRAIDSWM
                     AMYKNKAPWLNETTKSLNLPSAISSEVARLVTLELESEIVGNDFLNEQYQEVLKDIRK
                     YCEYACVAGGLVFKPYIGRNKIEVDYVQASNFFPVEYSSTGDITSAIFSEIKVKGDIK
                     YTRLEYHNWTSTSYTISNYAYKSNTARLIGYSNDLGKRIRLSDVPEWAELSEELVLEG
                     IERPLFAYFKIPQANSLENSSALGVSVFSRAVDLIKEADKQYSRILWEYEATEIAIDA
                     DSSMFRRNANGEYEVPHGKKRLYRMLEMEDGDNKWNVFSPAIRDSSLYAGLNQLLRKI
                     EFNCGLSYGIISDAQEIEKTATEIKTSKQRLYSTVKDIQKSLEDALEGLIYSIDKWSI
                     LAGFTPSLKYETTFNWDDSVIIDKDSELLAMQQDVASGLIRPELYIMKKYGVTEEEAL
                     KMMPDTGELVEDNPFDKDGI"
     gene            26066..27289
                     /locus_tag="ACOIAF_001264"
     CDS             26066..27289
                     /locus_tag="ACOIAF_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor capsid protein"
                     /protein_id="HGZ2668283.1"
                     /translation="MLTPNELQEIPKYFIGLFQSLEDFIIADIARRIAKVGNITDMAE
                     WQLIRAEEIGIADKVIKRKIAETLGISFETVDKLFEESAIKSIESDSALYEHAKLTPI
                     HLNSSEELKNYVSSAKEQTKGKLKNMTGTLGFCTIKKGRVISKKLTDIYIESLDLAQF
                     QVSTGVLDYKTAVKKAVKRLADSGLRFIDYETGWTNRIDVATRRAVLTGVNQMSQNIN
                     NKVIDDLDTDIVEVTAHSGARCEGAGIKNHKKWQGKWYSLSGKSTKYPSLRAVTGWGQ
                     GDGLGGWNCSHQFHAVVPGISVPAYTKEQLKNIDPPDIGYKGRTYTHYQALQYQRKIE
                     TAMRQTKRQLIAYESAGLKDEFTNASIKLQRQKQEYREFSKVAKLRLQNDRHQVLGYN
                     KSISQKAVYAGKKEK"
     gene            27291..27617
                     /locus_tag="ACOIAF_001265"
     CDS             27291..27617
                     /locus_tag="ACOIAF_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668284.1"
                     /translation="MDVDILGAKYTIVKDCTKDKEPLLAKFDGFMDDTVNKIVIAKME
                     HSDESLKDLNFYEKQVLRHEVIHAFLSESGLKSNSDWARNEEMVDYFAIQFPKMLKVF
                     MKLDAL"
     gene            27634..27915
                     /locus_tag="ACOIAF_001266"
     CDS             27634..27915
                     /locus_tag="ACOIAF_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668285.1"
                     /translation="MGKYRKKPVVIEAFKWLGSAEQKEEPTWIVRAIESGNVWIEQSL
                     GELSPRMYIRTLEGIHEANVGDYIIQGIKGELYPCKADIFRETYEEVLY"
     gene            28047..28619
                     /locus_tag="ACOIAF_001267"
     CDS             28047..28619
                     /locus_tag="ACOIAF_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage scaffolding protein"
                     /protein_id="HGZ2668286.1"
                     /translation="MKREFLKDLNLEDEVIEKIMSENGRDIEKYKKEVEKKKEELESK
                     NTELETANNKIKDLEKIDVESIKKEVDDWKSKAEQAQKDKELIENQMSEQTYNLNLDN
                     YLSNFKFSSNLSKEAVKIKMKEKGLEYKDGAFEGADDYIKELQANDPGAFISGNSVPK
                     VVSSSSGDAGESKVSLMEQMIAKNREMLNI"
     gene            28630..29703
                     /locus_tag="ACOIAF_001268"
     CDS             28630..29703
                     /locus_tag="ACOIAF_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage coat protein"
                     /protein_id="HGZ2668287.1"
                     /translation="MSLFDSKIFNGEVFGKYVETVPNLNRDELIKSGAIRRRGDLKAM
                     FSAQSGANYATIPMLGLIDGDPVNYDGQTDIVATTTKTFSQSVIVVGRAKSWAEKDFS
                     QDVTGGVPFMENVGNQVAKYWDNIDQKTLLAVLEGIFAMTGAKNLEFVNNHTFDITKE
                     TEAENQKVKAETLNSATQKACGDNKEKFALVIMHSVISTNLENLNLVARLKYTDPQGI
                     QREMKLGTWGGKLVLIDDNMPTREVAESSEGSNDGYTEYTTYVLGENSIDFEDVGTKV
                     TYEMDRVPGKNGGEDYLYSRQRKVFAPYGISFTKKAVATASPTDAELKNGANWELVND
                     SSNGSKTYINHKAIPIARIISRG"
     gene            29707..30039
                     /locus_tag="ACOIAF_001269"
     CDS             29707..30039
                     /locus_tag="ACOIAF_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668288.1"
                     /translation="MNMYIDYEFYSTLGGEIPQDKFNKYAIRATKYIDYNTFNRIEEV
                     TEEIKIAACEIADLIYKSNLEGDKEIQSESIGSHSVTYSANNKTIEQKAYDILKMYLD
                     SDLLYRGV"
     gene            30041..30475
                     /locus_tag="ACOIAF_001270"
     CDS             30041..30475
                     /locus_tag="ACOIAF_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6751 family protein"
                     /protein_id="HGZ2668289.1"
                     /translation="MFFKDNITLYNKYYDKYSDKTLYKRAYLIGVDYQGSKNIAVTDK
                     GLLSADSVKVIVPFSANARGKKYIDPFKYYELDEIEKEKFYTFKVGDIIVKEVVDFEI
                     TSVKPYTLRELQSKFDDVSIIKSVIKCDFGSIRMRHIELEAE"
     gene            30482..30829
                     /locus_tag="ACOIAF_001271"
     CDS             30482..30829
                     /locus_tag="ACOIAF_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HGZ2668290.1"
                     /translation="MNVHVDIDVARIMRERGLERGGKAQKFFTHEVRRLSDSYVPKQS
                     GTLKNTAREEVDKIKYIQPYAKIHYYNNSGRGSEGMGAGGKRGKYWDKRMLADNKVEL
                     IESVARFIGGSRE"
     gene            30826..31257
                     /locus_tag="ACOIAF_001272"
     CDS             30826..31257
                     /locus_tag="ACOIAF_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol resistance protein"
                     /protein_id="HGZ2668291.1"
                     /translation="MTIVESVREFIKKCPHLDEFAKSINVEFLAEEFTSYTIETVPAE
                     TIVKKFVNGDAIKQFVFIFASRESYGSDVMQNIENSQFYEQFADWIYRENLNGNLPVL
                     DGNKESMSLEVSTTGYPVQTSIDTAQYQIQLKLKYFEKGEI"
     gene            31260..32060
                     /locus_tag="ACOIAF_001273"
     CDS             31260..32060
                     /locus_tag="ACOIAF_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668292.1"
                     /translation="MAGIETIQRYKIADYLGILGDDGQSSWELMGAGFNTLDENPSAQ
                     SDSKTYINDRNSTSTIKSYQTQFPFESDLIKSEKAIMELYKIGRDQATGADAERDYLR
                     VELFLPVEGKDNTFKARKFRVSVEVSGLAGAGGETIIVSGNLNSVGNFVDGEFNTQTK
                     IFTEVGEEAPPAESLKVLTVNSIAGTNTGDTKVTVTPGLESGNSYKYKTGASVSVPAL
                     DADCSVGYTTWDGTEDITANEGDKILIVEVNSGNKAKKAGIATVTVKE"
     gene            32118..32480
                     /locus_tag="ACOIAF_001274"
     CDS             32118..32480
                     /locus_tag="ACOIAF_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6673 family protein"
                     /protein_id="HGZ2668293.1"
                     /translation="MLINNIELKDFDIYDADEHENIEKTIGLTITETGKVEKEATKGY
                     EMIRDICNLIFKCFDDIFGEGTSNKMFGDKRNLKVCSNAFFELIEEVNRQKQEGEEFF
                     ASAVSKYTPNRAQRRAKK"
     gene            32480..33085
                     /locus_tag="ACOIAF_001275"
     CDS             32480..33085
                     /locus_tag="ACOIAF_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage Gp15 family protein"
                     /protein_id="HGZ2668294.1"
                     /translation="MNMLIDVLPESVEIDGEEYKINTDFRISILFEMLIQDNSISDEE
                     KGENALLLFYPVIPKNTATAIDKIIWFYSCGKKENNVDDGCRGGTGSSKSQIYSYDYD
                     DEYIYSAFLGQYGIDLQDIEKLHWWKFKALFKSLKEDTEIVKIMGYRAMDISGDMPKA
                     QKDFYRKMKKIHAIPLPQNEVEKINEIEKALLNGGDISNLL"
     gene            33160..33801
                     /locus_tag="ACOIAF_001276"
     CDS             33160..33801
                     /locus_tag="ACOIAF_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HGZ2668295.1"
                     /translation="MGFKFRKSINLGGGLKLNINKNSVSVSGGVKGARVSVNSKGKAT
                     TTLGVPGSGLYYQETTQLGGGKKKNGKSAGGSESSRQAQQLLKIINDCANIVNTTKDP
                     KTFFYRYNMLLDKSYALVAIEDNLNFSGQSPSQMLDSIIAKKTDTINDFLTRYYNEMS
                     SKLKELKTEKARMNNAYKFSSLAIDYKEHLNNDNKTKLNRLYKQLLNDVKKQD"
     gene            33906..39209
                     /locus_tag="ACOIAF_001277"
     CDS             33906..39209
                     /locus_tag="ACOIAF_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGZ2668296.1"
                     /translation="MADGSIIIDTFLNTEDAEKQLNNLADTMKKKAKSALAIAGIGAS
                     LGAIGKQAIDFGDEYQKAMNGFESATGNAEAKAKGFGEALQQVYANNFGEDMGDISEV
                     MSLISQKLDGIDASNIQEVTESAIVMRDTFDMDVGESLNAVNSMMRQFGISAKESYNL
                     IAQGAQQGLNQNDDLGDQLAEYSTYYAQMGFSAEEMFNMMKNGAESGVYQIDYLNDAL
                     KEFNIRAKDGSNGTKEAFEALGFNADELTKKFANGGSSAKEAFVEVTTALNNLDDNVL
                     KNQIGVQLFGTKFEDLEADAVTALTNIEGSISSSKDKLEEINKIKYNSFGEAITGIGR
                     QMQVNLLLPIAEGLLPVLNDLANKFAKAFQNENTKNAIRSTAESIGALIGTVVKLISL
                     ALTPLINVITFLIQHGSLVAPLITGIGVAFAGLKIAGIARGIQKSFTDAQLAVRLFVT
                     GMAESGTTLTLYETIVGLVTKKISLAQVATNLWKGALTALGGPIGLTIVAVGALIAVF
                     VSLWKNNEGFRDAVINIWNNIKEVGEKIFGQIAKFFTETIPQAMSKFIDFAKENWQGL
                     ALLLVNPFIGGFKLIYDNCEGFRNTINKLFNSIKDALSKTMSFIKSNWQGLLLILVNP
                     FIGAFKLIYDNCEGFRNTVNSIFNKIVGIFKNVLSFIKNNWKSIGSMLLSPFVNGFKA
                     IYNSCVSFKNKVFSFFSSVVKGFVTFGKNIIAGIINGLSSGIGLVVDTVKNIGLTVVN
                     TFKKVLRIHSPSKVTTELGEFAGIGFANGIKNTSKDVVEATKELISAMEKNLETNKSK
                     FEKICEAITTALKNQYESQKDIQIKALDERLKVEEKASNDRLKVYEKEYNEKLKYLDT
                     ETNEKTNAIQEQIDAIDKQIEEEQKAEEEKAYNEKISDLNRKLATAKNQKEREKIQKE
                     IAETQADRQKKLLDEQRQQEKNRLKEQIENIKTEASNKKEQYKEEYDNQKEAESKKLE
                     LIKESNASQKEEIEKYFSELLEETNIQNEARRLLLQKNSEEIIKLLSEYNPHWQDAGQ
                     SLADSLLNGVNSKKQSIQEAVKEAINLKEIIPAQEQELDRLKKKLEEYEKLKENVSAS
                     SAGDGDLDTSVQDSSLDSGSSGLDSKELEEYANAVDDVKTSVEGLDLVSDELVKGTVP
                     KVGDSAGKLTESIKKGFGSIGGFFGGIEKWLRDTDKSINDWLSSASSSISDFFSKLKD
                     KFNAGIENIGGFFSGLGGKIGDSFNGLGDFFNGLEDKAKEGFSSIKNNAIEVFSGLGD
                     WFKETGSKIGDAIGTGLKSGLDFFTNTVPEWFAGLGDKIKEECGKIGGKISNFFNETM
                     PTIISNIVEWFKQIPYNIGFTIGSVAGFFVDLGAKLYAWATETLPEIINSIVEWFKSL
                     PEKIGEFLNITLQNIQTWGENFKVSVSEFFSVIWESITQFFTELPEKISEWFGTVVET
                     VSGWGENLKQLATDIFNNFVENILIPVSQLPGKLWEKFTEVIGKVTEWGSNLLQKGID
                     IASKFLENVMKFFSELPGKIKEKLDDIIKKVTDWGTNLVSKALEIAKNFFNNIYNTVS
                     QLPGKFKEWLDSIINNVISWGTNLVKRAEEAGKNMANAVKDALKDLPSKIMSIGKDVV
                     RGLWEGITGMGGWLKGKVFDFAGDIINGFKDGFGVHSPSIIMRDLIGRNLVKGVGVGI
                     DVETPELKEKIEKNISELTSKLKATVNFETSKIQANIVASTDFKAGKETAIMSSNKDS
                     EVNSNQAGIKLNIENFVNNREQDIENLFNEILFLAKRKGVI"
     gene            39264..40007
                     /locus_tag="ACOIAF_001278"
     CDS             39264..40007
                     /locus_tag="ACOIAF_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="distal tail protein Dit"
                     /protein_id="HGZ2668297.1"
                     /translation="MKIIFFIYDGRDSREFGLKIYNINDLSAPQIEIEKVNVPGKNGD
                     LLLEKGFGNFALTIECDIDARQSNIEEVATEIKKWLQGDISYKKLFLSNSDFYYLASC
                     NNKLDIIRTFKNFASCLLTFDCYPFKYAEEEIISLNVLNLKSTTITNFYRESNPVFYI
                     EATGDINIKINTQNIELRGITENGILSDLIIDSEIMNVYRENKNTNIIVNENSKLFSD
                     FPKLEEGENRISWEGDIKSIKINPRWNIL"
     gene            40103..>41037
                     /locus_tag="ACOIAF_001279"
     CDS             40103..>41037
                     /locus_tag="ACOIAF_001279"
                     /inference="COORDINATES: protein motif:HMM:TIGR01665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HGZ2668298.1"
                     /translation="MGVLTNVINDHVREEENGVFELEFTIYENSFLFKEIKTDRLVKA
                     DASPNFKGQLFRIYYISKNLGGYINVKAQHVKYDLLNNFIESLELNDITCEEALERVF
                     RACEEQNRFRGHSDIKARNTINIEMQSPYSAICEGENSLIQKFEPTAKLFFDNFDAYL
                     NYQRGESKNVLLAYRKNITGLEAEYDTQDIVTKIYPFATYEDEMITLTEKYIVSPNLN
                     KYATQKIVAIDFSSDEVSVEEELREKCKDYFKYNQVDLPKVLYKVNFVDLSTTVNYKD
                     YKMLETVNLGDEVIIRDFNLNINATARVVKTDYSP"
BASE COUNT        16173 a         4829 c         7913 g        12122 t
ORIGIN      
        1 aaaaaaaaaa tactatagta ttgaagttgg cgatttaata aatcatttag actttttaaa
       61 caacaagttt aataatatag aaaataaaat agatacagta aaaaaagcta ttgataatgt
      121 caaagtagac gattcagagt ttccaaacac tttgccagaa gtaccgattt tgacagcaga
      181 ggggttattc gcttttatta atctgacatg gacattcaca aataagtctt attacaaata
      241 tgaagtttat gcgtctcaaa ttaaagactt tgtaccagat acgactaatt ttacaaatcg
      301 aatttttgca gggcaggcaa gtgcttacgt tcaccaagct gaaccttttc aaacttggta
      361 ttttagagta agagcagtaa actcacatgg aaatgttaca gagttttcta atcaagtaga
      421 agcacagact acaaaaatgt cggatggagc tacatggata caagagggag caatcgcgga
      481 tgctcttatt gggcaactaa aacttgacag aggttggttt ggacagttaa aaggttcata
      541 tataaatgcg agagaattgg tggttgaaaa tgataacgga gttaaaacaa tggaagtaga
      601 tagctttgga gatgttcatt tcaacccaaa cacttttaaa gtttctttca atgaaataag
      661 ccctcatgtt cttattgatg atgagggatt gaaacttata agcgggaagg gatttacaag
      721 aatgaccgaa tcggggctat atacaaaatt ttatactggt tcagatgtaa cggagtcgtg
      781 gtatttgcaa gaaagtggat acacatactt tgactataaa gattcaaaaa tcataataga
      841 cttaccaaaa aaatttgcag gtaaaaattt taaagcaact tgtgcgataa ggtctacatc
      901 gacgaatgcc agttgcttaa gttatttcag cacatcggcg acagtaaaaa aaagtgatac
      961 aaatccacaa ttagagcttt ctgcatctgt gcgaggtgtg tcttacgaaa tgtcaggaag
     1021 ccagacaata ggctactggc tcggaatgaa ttttttttca gaaggattta ttacagtaca
     1081 atactatgtt tacgcttaaa attttcaaaa aaggagaaga agtatggtta ctatttttta
     1141 cagtaagaga actggagaaa tatataattg cataaaatca gaggtagagc aggattatac
     1201 ttgttttgca gatagggaag aggattatag acaaatatta gataaaatag ttgttgagga
     1261 tattccagaa attttaaatc ctcgtgatta taaaattgaa aacggaaaat ttgtattaaa
     1321 ggaaaaagaa ataataaaag aatttactcc gaaaatggta gagtaaaagg agtgataaat
     1381 ttgagagata gaatttatac agtagatatt aatactaaaa gttatcaagt tgcaaaatat
     1441 aagcaatatg ataatgggat agagtttaag attaatttat tagaaaataa tatagaaaaa
     1501 gatttaacag gatatacagc tatagcaaat tttcaaagac cagataggaa aatagtttat
     1561 cagagctgta ctatagaaaa ttctattgta acaactctga tagaaaataa cataacagca
     1621 gttgcggggg atgtaatagt agagtttact ttctacaaag atgatttagt tgttactact
     1681 ttttctttaa agattaatat agagaaaagt atagataaaa acagtattac agaagagccg
     1741 aaatgggatt atatcagcgt tacaataaat caagtaaaag aagtcgtcga aggcatagaa
     1801 gaaataaaag aaacagaaga agcaagaaaa gaggctgaga ttgaaagggt caaggagttt
     1861 aatagtataa aagaaacttt cgactctaaa gttacagaag ttacggacgc taaaaatact
     1921 atgattagtg acgttaatac tactaaagat actttaacaa gcgaagttac agatgccaaa
     1981 acagacttaa ttagtgtagt tactacagct aaagaaaata tgattaatga agtaagtgca
     2041 gttaaaacag aacttgaaac agcagaagct gaaagggtcg aagaatttaa tagtataaaa
     2101 gaaactttcg actctaaagt tacagaagtt acggacgcta aaaatactat gattagtgat
     2161 gttaatacta ctaaagatac tttaacaagc gaagttaata cagtcaaagc ggaagtgata
     2221 acagctaaag aaaatatgat tagtgaagta actacagcta aagaaactat gcagacagaa
     2281 gttacagacg ctattaatgc aatccccaca aaagaagagc taaaaggggt aggaatagaa
     2341 ataaaaggaa gtttagataa tatatcagca cttccagtca atccaacttt aagtgatgct
     2401 tactttgtaa aaagcgcaac aatagaaaac caaattgatt tatatgtttg ggacaacact
     2461 aattgggtca aagttcctga cataaaaata aagggtgaaa atggagatgg cttagaattt
     2521 aattgggacg gcacgcgact aggtgtaagg atagaaggac aggagaatta tacttataca
     2581 gatttaaaag gtcaaaaagg cgataaaggt gatagtattg aatttaattg gaatggcaca
     2641 cgattaggta taaagataga aggtcaagaa aattatagtt atacagaatt gaaaggggag
     2701 cagggttata ctccaacaat aggagaaaat ggaaactggt ggattaataa tatagatacc
     2761 caaaaaccag ctagaggggc atctttgagg attttaggaa aattagattc tatagataac
     2821 ttaccattag accctactat cggagattgc tggattatag ggagaaatat ttatatatat
     2881 caaactaaat gggaagactt aggttctcta gcgggcgtgg acggcaagaa cctagaattt
     2941 aattgggatg gcacgcagtt aggcgttagg caacaatacg aattagatta taagtatata
     3001 gacctcaaag gtgacaatat cgaatttgca tgggatggca cacgactagg tgtaaggata
     3061 gaaggacagg agaattatac ttatacagat ttgaaaggtc aaaaaggcga tagcattgaa
     3121 tttagttggg atggtacgga gctaggcgtt agaatagaag gtcaagagga ctatagctat
     3181 acaaatctaa aaggagcgac aggaaataaa ttagaattta attggaatgg aagtcagcta
     3241 ggtattagag aagaaggaca aacggaatat atatatacag agcttagagg ggaacaaggt
     3301 tatactccag caatcggcga aaacggtaat tggtttataa atggagaaga cacagggaaa
     3361 gcgtcaaaag gaaaagttac atggaatgag ttattagaaa aaccgaaaga gttagattac
     3421 attaaaaaaa gcacgacttt taattcagac ggctctataa cagatatttt agactcggtt
     3481 agtaaaacta taactaaatt taatgcagat gggactatag tagacgaaaa gtatatagat
     3541 aatgtattag taagtaaagt aaaaacgact tttaaaggta atcaaataga agaaataaaa
     3601 gaagaggtga gttaatgagt tgggcggaga catacaaagt gaatagtgac ttgcaaggcg
     3661 agccacttaa ttttttaagt tatttgcaag acataaaact aaatggatta gatagttatg
     3721 tgctgtttat tggaaatgct aggatatggg aagaattata tttaaatagt ttatatttat
     3781 tttctgatag aggaataaga gaaacagttt acacagcttt ttcagaaact gatattgata
     3841 atttatttaa taaaagcact aagctagggg aacaattaaa tgccttttat agaacagata
     3901 tatttagctt aggaaatgct gataatgtag taaaagaaat gactatagag cattataatt
     3961 cattagagga aaaatttaaa gcagggtatg atagatatgt tacaagagaa caagaaaagt
     4021 caactatagg agcatggttt aattctactt ttagtttaaa taatactggt ttagaaagtc
     4081 ttacaaccat agaagaaata ttagcaaatg tggaggctac taatgcaata cttaataata
     4141 gtaatgcaat agtagcttta actatgtgta aaacaagtat ggacgcagtt gctgcaagcc
     4201 caaatgcaat ggatttgcta ggacaatata ttttaaaagt tacagcagag ccagcagtta
     4261 taagagcgat aattaaaaat gaagttatta gagatgcaat tataaatagt gatgaagcta
     4321 tgacacaaat ttcaagtaac gaaaactccg taatggaaat atttaatgac ttagaagcta
     4381 caaaagtatt agtgcaaaac caaaatagca taaataaaat attaactaat aatgttactg
     4441 ttgaaaaaat aatccctaat ttattagaaa tgaagtataa tttacaaact tctttaaatt
     4501 atataaatac aataaaatca aatattgctt ctggtaaagg tcaaattatg gcaataactt
     4561 ataatgaaga aatattcccg attttaaaaa atgcagcaaa aaattatgat ggtatggaaa
     4621 acacgcgtaa tatattacaa cgagatattg aggaaaaaat aaaaatttcg gatgcaattt
     4681 tagaaagttc aatcgcaatg acaacttttg caaataattc tattatagta aataaagttg
     4741 gtgacagagc gggaattata gaaagtattc tcggtcaaac agtgccatta aatgctttta
     4801 tgaaaagtac aacagctata aaagttttag taaataaagc tactgcattt actaagatag
     4861 taaataattc aacagctttc aatgctatgc ttactatttc agggaatata agttctgttg
     4921 caaatagttc aacagctatg aatataattg caaacaacgc acaagttatg gcaacagttg
     4981 caagaaacga tacagttttg actgttttta taaatgcttc aacagctata actgcaattg
     5041 cgggaaatgt gacatctatg tcggcaattg taggctattc gggagctatg aataaaataa
     5101 caacatcagc atttgcgctt aatagaatat tcaagagtac tataggcaag gatatattaa
     5161 ttagtaataa tgcaatttta caaacttata aaaataatat acataacaca ttgagaagct
     5221 cctctggtca atttaggtta attacatccc caacggattc ggattctgca ccaactcaaa
     5281 ttataaatag ttcttatgta ggagtaactt attgttatgg atatagaggt ggaactggta
     5341 aatttgctat cgtatatcat ggtcacaaaa caagtgcaga agcaggcaga ggggacggtg
     5401 gcaaagatga aaccaagaaa tttataacac taggtgggac taaattcgtg gaaagtggag
     5461 atggatattt taattattca atatatcaag caatttaatg tttataagtc atctaaaaga
     5521 ttagattaat agtctaatct ttttttatat aaaaaaataa ataatcaaaa ttaggaggaa
     5581 agttatggaa caattattaa cagaattaag tagtttagga gcaataggca tattatgcgc
     5641 tttgctattt aaaaacacta tgcaggagaa aaaagaagat agagatatgt ataaaaagac
     5701 agtagaaaat ttcatagaac tatctacaca gcaacaagag ataaataaaa atatacttgt
     5761 tgaaatggga gcaatgaaaa cagacgtaga agaaatcaaa gaagatgtaa cagacataaa
     5821 agacatgtta cagaaagaaa gtctttaaat gaaagtagca ctaacagcag ggcatacgct
     5881 aacaggaaaa ggaacaggag caacagggta tataaacgaa ggaacagaaa ataggatact
     5941 tatggatttg gttgctaaat ggcttaaaaa aggtggcgct actgtatata gtggtaaagt
     6001 agataagtct aataactact tggcagagca atgtcaaata gccaataagc aaaatgtaga
     6061 tgtagctgta caaatacatt ttaacgctga ccatacaact ttggataaaa tgggtacaga
     6121 aacaatttat aaaactaata atggtaaggt atatgccgaa agagtcaacg agaaactagc
     6181 aacaatattt aaaaatagag gcgcaaaatc ggacgcaaga ggtctttact ggcttagtca
     6241 tacaaaagct ccagcgatat taatagaagt gtgtttcgta gatagtaaag cagatacaga
     6301 ctattatatt agacataaag acatagtcgc taagttaata gcagaaggta ttttaaataa
     6361 gacaatagat aataaagaaa atagtgagga taagaaaatg tataaacata caatcgttta
     6421 tgatggagaa gttgacaaaa tccccgcaac tgtagttggc tggggctata atgatgggaa
     6481 aatattaata tgtgacataa aagattatgt accaggtcag tcagagaatt tatatgtcgt
     6541 tggtggtgga gcatgtgaaa agataggttc tattactaaa gaaaaatata caatgataaa
     6601 aggtaatgat agatttgata cactttacaa agcattagat tttattgata gatagattaa
     6661 aaggtagtaa ctatctctag ttactacctc ttttcatttt tttaaaatta gtaaataaat
     6721 atcttacttg ctgtattctc tttcaagaga atctatatta aaaacccatg actttccgaa
     6781 aagtctacag tcaacaccct caacaaattt accattttta atgttctgct tcaaggtact
     6841 ttcagcctta ttgtactttt tagatgcgtc gcttaaagaa ataaaattac taaaattatt
     6901 tagttgctct atatatccat tataaactcc cacgacaaaa ttaactataa cctcgtaatt
     6961 atctaagttt tcaataattt cagcaggcat aggtatgtct aacttcataa aagcctttaa
     7021 aattatttcg aatgctttgt ttctatcttg gtatctgtaa attgcatcat ttaattctaa
     7081 aattttattt ctacttaata cattctcact tccgatttta aaacctaaat tatatagctc
     7141 tttatttaat tttgcattca taaaattcac tccttgtttt gtttatataa ttattatagt
     7201 cgatacaaac aaaaaaagca ataaaaatag tcttaaaaaa tatttatagt ctttttagac
     7261 tattttttat tgacaaatag tcttttaaga ctataatata attaaagata aacaaaaggg
     7321 ggatttaaaa aaatgaaaaa gataactaaa aaagaaataa tagaatttgt aagagatgta
     7381 gtagaagaat accgtgattg gaaattagaa aaatgtggct tctatataaa ggataatgaa
     7441 ttaaattctt ttgtaagctt tgaaggaaaa ggaattgaca taaatgtata taaagaaaac
     7501 tacgacgaaa taatttacat agaagattat ataaaagact acaagcgtaa ggaatataac
     7561 ttaaaagaaa tagattctat catatacgaa gatgtaaatg aaatgattaa taactataat
     7621 gaaaaataaa aaatgaaaat aaaataaaaa ttactggagg aaattaaaat gaataaacaa
     7681 aaagctagaa gatttttaag agttatagat atgaatatgg ataaaataga agaagaagct
     7741 ataaaagctt ttaaagaaag ttgtttaatc aaagaggaaa atgatataaa aatttttatt
     7801 gattttcaag gaaaagttga agcgatagca atccaggctt atactaaact tttagatgat
     7861 aataaagaaa ttaatatttt cacatcaaga caaatagaaa ataacttaga cgatatgttt
     7921 ggagaaattt gttacgtaaa cgattatgaa gaatttgtaa attggtgcga agataaatgc
     7981 gaaaacttga attgggatag ttataaaaaa ttcaataaag aaaatttcga agaaattgct
     8041 gaaagaaata tagacgatag cacaccagtt tttttagaag aattacgaga aggcattgaa
     8101 agttgtaaac aagaattgca aaatatagtt gaaaattaag tagaagagta ttattattaa
     8161 tgtatatagt atcgctttta agattaacta cgagggattt aaaatatttt ttaatttatt
     8221 tagaaatgat attataataa gaattttcag attaactaca agggatttaa atgtcaattt
     8281 aatttataga gaaatgatat aatatcacat tattttacat taactatgag gaaataaaaa
     8341 cagtgcatcc aataaatgca ctgtttttta atgtttagtt attacgataa cttaatcctt
     8401 caagtataaa ccctacattt tccgtcattt caaattcgct actttgcaat attccttccg
     8461 taaatttgtt gtcatttggt aatgttattt ttttccttgc cactgcttct atgattgttt
     8521 ggaatagttt aattttcata tccttatcaa ctccggcgta tcgcaaaaaa tttacaactt
     8581 cttgtgaaaa cttatttaat tcagatttag tcattttttc tgataaatct tttccaattt
     8641 gcaaataaac attcatcttt tccaacccct tttttagatt atagttaaaa catattattt
     8701 aaacaatatc gatatattgt aataataata ttaaaatatt acttaaatat tatatataca
     8761 atatttaagt aatatctaaa caatataaat ataatattat ttactttttc ttagtcttat
     8821 ttcttctatt tctctcattg tatatattta aattctcttc ttttatagtg acatcagata
     8881 aaatttgagt tagtatatca cttaataatt tactaacgtc gctgttcata tcaatagaaa
     8941 aagattttaa tttgtttaca agctcctcat caagcgttgc actcatagtc acttttttac
     9001 ttttcttatt aactagatta tatatgttta atgtcttatt gtctgtagtt gtagaatcat
     9061 tttgtacgat tgttccattt ttcaaaatcg gtttttcttt atcaactaaa aggtctctgt
     9121 tgaaacttgg catctataac actccttttc ttttcaattc ttctaacata ttagtaaatt
     9181 cctgtctgct gaaatgctct ttagttattt ttgtataatc tgttagagat agttttttta
     9241 atagtgcctt ctctataaac tcattttttc taatgtatgt atctaacata atatccctca
     9301 tatcgttaaa cccttccaga taagaatcga aagtatcgct aatttgagtt ttcttcttct
     9361 caaatccaac taaaatagta ctttttatat tatcctcctt atcaaaatag gttctatcaa
     9421 catcccagag ttgtttaaat aattctgctc ctcttaaaga agaaatgttt ttatcttgaa
     9481 ttggaataat tatactatct gctaagaaaa gcacattttt agctgtcaaa tcgtagcgtg
     9541 gagataaatc acagataata taatcatact cactcaaagt attaaagttt tgcatgtacc
     9601 agcgagctaa aaatttttct ctggctggta aaggtgatag ctcttgttca aatctagcca
     9661 tttgtatgtc agaaggaatt aagtctacat taatgtaatt ttcattaggt gacttgacta
     9721 ttgcatcatt agcactaatc ccttttaaaa tatctaatgt agtattatca ttatgattta
     9781 tttcataaac atattgtgtt agatttgctt gtacatctaa gtcccataag agtatttttt
     9841 tatctttatc tttcgcaagt tcatacgctg tcattataga cgttgttgtc ttatagatac
     9901 ctccttttat gttaaagtac gtaagtagct ttgtattagc cattttctcc acctcattat
     9961 tatatttata atattataac aatattgtag tattgttttt ataatattgc aacaatatat
    10021 taatattgtt aattacaata ttcaaataat ataaatacaa tatttacata ttgcttttac
    10081 aataccaata tattatatta aaaatactaa aatattgtat atacaatact aaaataatat
    10141 ttttataata ttacagtatt atttttataa tattacagca atatttttat attatagtaa
    10201 aatttaaaaa aaagaaggct aagaaaaaaa gcaaccttct tttatagaaa aagtcattca
    10261 ttcttagcct tttcaattat tatcctgtcg ccctcgaatg ttgccgtaac attagggttt
    10321 tctcttgtta tgcccatttc atcagcccat tttttaggaa cactcattct aggagtaaac
    10381 gacccactcc cacctttgtg aaagttaaac tttaaatctc ttttttcttt ttccattcat
    10441 tagctccttt tttgctgtat tacaaagaaa agcaataata caacactaaa aactaaaccg
    10501 agcatgttgc ttgtactaga gtttaatact atagatacta tatttaaaac tatagttatt
    10561 aaaactaaaa caatacaaat cttataaaat ttatcactca tgttttttta gaaatgtgtt
    10621 ataatattag aaagaagtca cctagagaga agggctattc tctctaggct tatccttaca
    10681 actcttttaa aattcttaga attgcaagga ttatgttaag taataataag atgaaggaga
    10741 aaactttttt aactatgtta aatttgtttt ctcttttctt tttacttctt tctaatctcc
    10801 ccattcctgt gcctcctttc ttttattttt ttgctttctc acctccttct ctataaatat
    10861 attatactat gacgggtcgt caaaagcaat agttttctcc tattttttaa acaaattttg
    10921 gtaaaaagta aagattttag tatattagaa atgatgtaaa attgtatctt tttttaaaaa
    10981 atgtgatata ataaaagcaa ggaaataatg atttacttta cacaagagta gctatttcca
    11041 atgttttgaa aaataactta ttttcttaaa ccactcttat tggcgtttga gtggtttttt
    11101 aattttatcg ttcttaaaat aaactttgat aaaaataaaa aaaatagaat actgaaaatg
    11161 atgtaaagtt gtatctttta aagaaaatgt gatataataa aagcaagaga actgtattct
    11221 agtcataaga gtagagtttt agtctaaaat aaaaaattat ttaaactttt tgaatttgat
    11281 tctcaaatca aattcccaac cactcttttg cgcagagtgg ttttttactt gtctaaataa
    11341 tttgctaatt atatagaata ttaaactagc tataacacca gctaatacat taagtaaaaa
    11401 attatccata cttcatcacc tccctttgta gcgttgggag gataatcttt tgtgtataga
    11461 ctccactctt ttttagaata tagattgctt tctcttgcta aaattattat aacatatatt
    11521 tgcattttaa aagacttagt ttgtatacta agtcttttaa aatgcagtat tttagaaaat
    11581 atatttttaa aatataattt attttcaaaa gagtatattt ttttataatt taaatgtgct
    11641 ataattataa tcataacaag cagaaaactt ttttttaatt ggtttaaaaa aatctacaaa
    11701 aaataaacaa ggaacatgta cttgccgtca aacaaaccat gttccttaca ccggaggtta
    11761 gacctctctt aaggataata tatccttgct tttctatact tatatttata ttataagaca
    11821 aagtgtagaa aaacacaagg tttttagggt aattttaata gtaaaattat tacaaaaaat
    11881 caaaaaaaga gcgtcagcct ccaggtgaaa ttggaggggt aaagatgaag aatgttaata
    11941 aaaataatat agataaatta tttaaaaatg attgtgactt agatagagca gtaggagaaa
    12001 tagaaaatta cgcaaagttc gagagacaag aaacgcataa aataaaaaaa tatatagatg
    12061 aaaaaaagag tggagtatat aatgcgatag cgagagcagg agcaggagag acggttcttt
    12121 tacttgctga aacagggaca ggaaaaagtt atagctttat aaatgcactt aaaaaaatga
    12181 atacaaaagc actattcata ttaccaaaca gtgcaaatgt cgagcaagct atgtgtgaat
    12241 atgacatacc aggggcatac gatttgatgc cagcccctaa ggcattgaaa gaaggaaatc
    12301 tatcagtttt tacttgggat aaaacagaac aattactaaa cattgatttg agcgattaca
    12361 taatcgttgt tgatgaaata catcaaacgt atacagattc ttacagagaa aaagcgataa
    12421 aaaatttaaa taatatagtt gcaaaatgca aaggaagaat agatataaca gcaactccaa
    12481 ccaaattaga gtttgaaata tatgactaca taatagaata cataaaagaa aaaaatacta
    12541 aatatgatgt aaaactttat gacaatataa acttagataa aatgatagag attatcaata
    12601 aatcaaaaaa tagtgcagtt ctttataata ataaaaagga tttaaaatat ataaatgaat
    12661 taacaacaaa aaaaggtgac atagtttacg caaaaataaa ggctaaaagt gaattgtaca
    12721 agagaataat acagcactct gatatgggag attatgaact tttactaaac acaacaacaa
    12781 ttttagcagg ggtaaatata aaaaataaag atataacaga tataataatc gcaggtttta
    12841 aagacccagc aacgataaaa cagtatgttg caagatttag agaattaaaa cgtgtaaggg
    12901 ttcatatttt taataatttt aatgatacag aggcaagtaa caaggtttat gaagttaata
    12961 atataattaa tattaatttt aaaagagcaa ataaattaaa agcattatat aacgagttaa
    13021 atagtaaaag agcaacagga tttgaaactg ttgggataga ccttgttcct tttagtatag
    13081 attctaatgt atactattca actaaaaata aatgttatga aatagataag ttatttatta
    13141 taagcaatac ttacacaagg tattataaca gcagaacaat agcgagttta aaagtacttt
    13201 tagaagaata tttcaaagat gtaaaaatag aaaacataga agaacaagat gaggaaacaa
    13261 agcaaggaat aaaagaatat aaaaaagtct taaaagaaat caaaacagca acattaaaaa
    13321 ctttagaaga aaataaaaag tacctagtcg gctatgagca aattaaaagg ggttctaagg
    13381 actttaacct catgagctat atgaattata ataagatgtc tgaaaacgac tacaagcagg
    13441 catacattaa gtataatata gattatttag tcgacaaagc agactgttac agcgaaattg
    13501 aactattttc taaatatgta ctagaacata actatagctt agaattatcg tggaaacttg
    13561 ctaacatggg gaataataaa agaggaaata tatttaaaaa aataaataat cttatataca
    13621 gagagttaaa agaagaatac ccgaacctga taaatgataa cttgatagaa gttgttgcat
    13681 ataactatat agataaactt tttggaattg gaacagctta cacagaatat aacttacaag
    13741 agttaagtaa cgatttagcg ataattttgg gggataattg gagatttgcg acaaaaaaat
    13801 tagggaatgt tctaaagaat atatttatag taaaagccaa acaacacaga aactttgaag
    13861 cattagaaaa ctattcatat tacaaaaata ataagccgaa tactgatttt atagacagtg
    13921 acagaatacg aataaaaaaa attgaacgat acataacaat agaggacata gaaaaggatt
    13981 taaaactaga gaaaggcgat ttaactttaa gaaatgcaat aaaaattaga aagaaaaaat
    14041 tattagacaa cttagatgaa attgaaaaag catttttaaa taaagagtta gataaagaac
    14101 ttagaattga aagagaaata gaattagtag agcaactaga aatttgcatt taagaacaaa
    14161 aagtcaccga agtaggattt aatatatata tatatatata aattcctact tcggtgactt
    14221 tttgataatg agtcatcaat ataattagca tacaatattt aaagaaatgc tataaaacta
    14281 gaaaacaaag gctattgagt aaattggatg aaatttaaaa taggaatgta tacaattaaa
    14341 agaggaaaat tcactttagt gtagaaatat atataaaaaa gcaaatatag ggggatagaa
    14401 tgaaaaaaat attattatgt gtcattattg gaatgttggg attaacctta gttgcctgtt
    14461 cgtcaacagc aagtggagat aagattgatg gaattgaaat tgttgcaaag gaaacatctg
    14521 cggaacaaat caaaggagat tgtaaccagg atgggagcta taatgaaaat ggagagtatt
    14581 tccagatggg attggataca gctaaggcgt cagattatga atataaaata gttaatgtgt
    14641 cagttaaaaa tgctagcgac gaggctatta aactgtttca aacaggctgg aacgctacag
    14701 ctaaagacgg ctacgaattt gaacatataa gggttacaga taagttggaa aatcaacaag
    14761 tacctgcaaa ttattctttt gatgcacaag ttaagatact tgttgaaaag aaaatgaatg
    14821 taaaagagat aatacttaaa tataatttaa aagactatag taacttaact aatgctatga
    14881 aagacgcgag ctctggagct actaaagagg atatacaaaa gaagtatcca gaattgtatg
    14941 acgataattg gatagaattt ggaactataa aatttgaata aaaaaggaaa aggcttaatg
    15001 ttttgcatta ggtcttttaa atatagtaaa cagcattgtt atgttttaat ttttgtggaa
    15061 ttattttcca gtagaatgaa tggcgaaata gtactataat cgtagtataa ggtataattt
    15121 tgtcataaat cgtacatatg ttcttgaaaa taatacaaaa ggggagtata atatttgata
    15181 gttacatact tgatacaatt attatcttat tatttttttc acagggggat tgggtatatg
    15241 aaaatcgaat atattaacac ggaagaatat actgaattta ttgaatttat tgaatttatt
    15301 gttagtgcta aaaaaactaa aatgaaagaa tttatataca taaaaaaagt tctttttgaa
    15361 ttagaaaaag aagatagata gtatctatct tctttttttt attcagagtc taacgatttt
    15421 tgcattaatt tttttatatg ttctcttttg cttggcgtta attttaaata taattttgcc
    15481 atttcctcaa tctctgggtc taaatctaag tcaattagtg ggtctataaa tttcttacct
    15541 attccattca ctagccaatc cttattcacc ttgtaaactc tgcaaatatc atctattgcc
    15601 ctgtccgttg gtattctata ccctttttcg taagaagcaa tctgactttg acttaaattt
    15661 attttttcgc caaaagcctt ttgtgatata ttttcaaaac ttcttatttc tgcaactctt
    15721 tcgcatatgc caggtatttt accctcgtta ctatttcttt ccttcaaagt aaactcctcc
    15781 ttttgcgata ttataacata cataatatca caaagcaatg taatgcgatt taaaatattg
    15841 caaaaatatt atttttatta tttagtgata aatagtattg caaagtaata aaaagtgtat
    15901 tataatcttt ttatagaatt aaaaaaagga ggggcaaata tggaatataa cataccatat
    15961 gaccaaaaag acttcttaga atttcttaaa ttaggtgaga ctatcatggg gcaaaatcct
    16021 ttgaaatttc aaagattaat cggacgcatg caaggaatag ttgattgttt agaagatgaa
    16081 gaggagcaat aaatgctaat taaataaaag cgtactttga cggctacgct aaaccaatta
    16141 aaaaaagtaa aaaataaata aggaggctgg aaaatgataa aagttatttt atgtagtact
    16201 atcgacgaat atatggagta tgaggacgta ataggagcaa aggacagcta tttaatggat
    16261 gttatcatag gtggcgaatg ttcagaaatt gatgctatag aaactttaga aattttaaaa
    16321 aatagtaaag cgagaattgt atcagaagca gaaaaaactt gtttaaaatt tggtattggt
    16381 aatcaaattt caaaggcgat agaacaaggt ataaaagagg ctccaaaact aaaaaagggg
    16441 atacattgtg ataattgtgg ggcaagcgta ggcagttcgg aggaattaac ttcaatgcca
    16501 gttttagaaa attataacaa caatcaatcc gtttctgacc aactaggaag tatagacgta
    16561 tgcccaagtt gtttgctaga aataacagac ttactgtaat tccctgtttt agttgaaaag
    16621 aaacaaaaag taacagcgta agccgtcaat aaaaaaaaga agggaggatt taaaaagatg
    16681 attaatttag aaacattgtg taatggagaa acaaaagaaa agattgaaaa tgggttcatg
    16741 gagatattta aaaacataca agaccctaat acaccagcga catctacaag gtctttgact
    16801 gtaaaggtta cactaaagcc aggcaaaaat cgttctcatg ttaatacgca aatacaagtt
    16861 attccaaagc tcgcaagtgt cttgccatct gaaacggaca ttatagttga aaaggacttt
    16921 aggactggag aagttaatgc aaatgaatat ggaaaccagt tgccggggca agtaaaatta
    16981 ggcgacttgg agagtaaaga aacaagtgta acagaagaaa cagaagtaaa agaaagtgaa
    17041 gaagataata ttcgtaaatt taagagttta aaagacttat aaaaactaaa aaggagtgtt
    17101 taatatgtta acagatttat ttaacagggt aatggaagtt ggagcaagaa aaatagaaac
    17161 aagagaaata aatggagtta catatacgac tgaccgttta attccagtat atcccaaaac
    17221 agttgaggaa attgaattaa atacattaga cggtttaata gagtatataa aatcagaggt
    17281 tgacaaagat gcaaaaggca atagacttat agttcaaatt aaaagcccta aaagtgttag
    17341 attatgttct gaactgctgg aaggtggtaa aaggaatact tatgttgatt gcaaagcgtt
    17401 attacctcat attaattttg attgctcgct tgattcagag gaatttaata ttatgctaca
    17461 atcttctttt gtagatacgg aagataaaaa tttgctttta aaagtagctg ggaatgtaaa
    17521 agaaactagc gtaaaaactg tacaggatga tggagtatca caagtggttg caatgtctac
    17581 aggtgtagca agtgtagagg atgtaatcct gcctaataga gttagtttga agccttacag
    17641 aacttttaca gaagtagacc agccagagag cgagtttatt ttcagagtaa aagagggcat
    17701 tacatttaag ttaatcgaag cagatggtgg agcatggaga cttgaagcaa taaagaatat
    17761 aaaagagtac ttagagaaga aattagaagg tattgaaaat atagatataa tagcataaaa
    17821 aaattgaaga ggctggacta aataaccagc ctcttttaaa aaaggagtgt ttattttgta
    17881 taaagatata acaaaaatgg gggcaattga acgtggggaa tacgttggta atgttgctct
    17941 tgaattaagg gaagaaaatc ctcatcttag taaaatagat ttaattgagt tagctcttaa
    18001 gaaaataaga gaggaaagtg aacaggaggc tggggaatga aaataggtaa atatttaatc
    18061 attccgcagg aacaggaaga ggcaagaatt gaggacttaa aaaatgaagt tagaaaaagg
    18121 gatgatagaa tagagtttct tgtatatgtt ggaaataaaa atcgtgaatt gattaaaaac
    18181 ttagaagaac agttaaaagg gtataaaagt gaattagatg tatatagctt gtttatagat
    18241 tgtattttaa ataaagaaac aaggaaagcg atagcgattt ataacagaac taaaagcatt
    18301 aggattaagc agaaatgtct taattctata cctataaaaa agggttaagt tgaatgaata
    18361 atttccttgt aggaataaat gaggtgctta aatatgagtg aatttgtgca gtgttgttgc
    18421 tgtgaaagaa caataaatat agaagaaaat aactatgtac agtatgaaaa agaggctcta
    18481 ggtttagttt ttacactgta tttctgctta aattgcgtag atgaattaag cgaaatggaa
    18541 taatttttaa attacgaatg gaggtatata gatatgataa tgaaaaatgt tttaaaacta
    18601 ttagcaatgt attgcttttg cccagagtgt ggaagtgatg aacttggaga aggtgaagga
    18661 agtttaatcg ttgatgagta tacatttcat agaaagtgta aatgtggatt tgacgtgata
    18721 gtagatgaaa gggaggacaa aatataatga attttttagg aatgataatt ttgattatat
    18781 ttgcattttt tattggtcgt atatatgaat atagagttaa tttaagagaa tgtgagaatt
    18841 gtaagggaga ggttggaaat gaataacact acactaattg gtagactaac aagagacccc
    18901 gaattgaaat atataccagg ctcgggaacg gctgtatcga cttttacaat agcagtagat
    18961 agagactata ttaaaaaaga tggcactaag gaaactgatt ttatacctat tgaggtgatg
    19021 gggaagttag cagaagtatg tgctaataat ttagataaag gtagattagt agcggttgaa
    19081 gggtctataa gagttgaaca ctatgaaaaa gatggcgaga aaagaactta tacaaaagtt
    19141 catgccaata aaattaaatt tttagattat aaaaaggatg atactggaaa ggcatatatg
    19201 tttgagccgg gaggcttaga cccacaaggc ttccaggcta tagacgacgg tatacctttt
    19261 taagaaggaa gtggttgaac attgaatgaa aagtggaaac aatatataga aaatgtttta
    19321 gaatcgagag gttattgcat aaatagaatg ccatatgtat attcatatga ttttcttgta
    19381 aatagcaata ttaagataaa tgtagaaata tctaatcttt ataaaaatga ggaaaatgaa
    19441 tatcactatt ttgaattaga aaaagaatat catagttgcg atatttatat ccttattgca
    19501 cttgatgaag tcggaagtgc tttgaaaata ttagtaatac cagctagcaa tttaatggga
    19561 caaaagcaaa taattattaa taacaaaagt aagtatgata aatttgattc aaggtttgaa
    19621 cttataaagg tatatgataa tttttataat gaccttgatt gtgttatgta gaggtgtttt
    19681 aggtgtttaa aaaagtagaa aaaaaattta gtggttcagt taatccgaat ttcatggaag
    19741 atgacctaac ttttaaaaat tatttagcgt tatattgttg cataaaaccg gttaaaattc
    19801 atggaaataa aaaggttttg gctagttcca ctaaggcatg cagtgcactg aaaattagag
    19861 aacatcagaa aaaagcgcgt aaaaaggtaa aacttataaa tgcaaaaaca ggtgaaataa
    19921 aagaaatggc tatagatgaa gcagaaaatt ttttaaatgt taaaaattta tacgctgtta
    19981 tttggcgagg gagaccaact cgaactggtt ggtatgtaaa ggatgttgga aatgaaggag
    20041 attaaatata acgttatata tttcaattca gaagctttaa aaatggataa gagaagtttt
    20101 gacgctttaa aagatgctag agcatttaaa aaggaaaaag agaaaaaata caaaaatgtt
    20161 gagattatta aaaagacaat tatagaaaaa ttgataatgt aggaagtgaa tttatggcaa
    20221 aaaagaaaag gatttgttca tggtgtggta agtcgttttt tacagagtcg aaaaatagat
    20281 tttgctgtaa atcatgcgaa agaaagtata aaaaagacaa agaaaaaaac gcaagttctt
    20341 gcttagttga agaaaccaaa gaatatttaa ttgaactagg aaataagaaa agaagagtag
    20401 atattctaag aaaagagata gattttttaa aagataaaga gttttataga gagataaatt
    20461 tcaatgaact agggtttaaa atacattcta gcccgaaggg catagatgaa atgattatga
    20521 ataatgagga taaaatatca tttaaggaaa gtgaaattga ttttatagaa tatagattga
    20581 gactaatgga tatttacata agtgaactta cagaagagaa gcaacaaatt ataaaattta
    20641 tgtattttta tgatggcgca agtaaacttt ctaatgaaga aattgcctca aagattaaat
    20701 gtagtcgcag taatgttcat tataaacaca aggaagcatt gaaagaactt gccgaaatgc
    20761 tttttgaaag tggaagtctt ttgtagaaca aaaaaaagac ggtttttaag acaaagttag
    20821 aacactcttt tacgtttata tagtgtataa tattaatatc aagaagttta aaattaaata
    20881 gatatagatt tgaagcctgg caggaatgtt gggcttttta taatgcaaat taatatttta
    20941 agggggttga gaaggtgaat agaagtgggt agtaagaaac cagctaaccc agtcaaagac
    21001 aaacgtatgg tcttaaatat acaagaatac ttaaaagaaa agaatattag agattatgtt
    21061 ctatttgtgc tgggaatagg tactggctac agggcagggg atttagttaa gctacaggtg
    21121 agggatgtta gaaatgcaat agacgaaggt tatttcttaa taatggagag taagaaagaa
    21181 aagactaaga acataagaaa gaaaaataaa aaacctagaa aagctccaat agttcctaat
    21241 ttagaaagag tactcaaaag ctacatacga gataaaaagg attatgaata tatgttccca
    21301 tccaggcaaa aatctgttac tccgtatata ggtgttgaga gggtaacagt gatactgaaa
    21361 gaagcaggta gatattttgg acttaagcat ataactgcgc atagtatgcg taaaacatac
    21421 gcttatacca tatacgaaga aagtggattt gacattataa gagttaagga aatgcttgga
    21481 cattctagta tagaagaaac caaggcatat ctaggtctta atgaagaaca atatcaagaa
    21541 tatagcatgt ttttaaatga cctaataggg tgattttttt attttgtctt tttgaatgtt
    21601 ggaaaaataa agacctgtac attcaatgta ttttttttgc tatatataaa gtaggaaaag
    21661 taaaaagtga atgtctgatt ctctaagaaa accccatatt cgttaaaata ggcgttagcc
    21721 attataaata ctgggataga agctatttaa cgttaaaatg ggtatggtga agcttaagga
    21781 catacggaca aaacttaaag gacatggaaa aatagagaat gaggttgatg taataaaaac
    21841 tataaaattc acaggcaaac aattcaatta cgccctttta caagagtaat ggcaatattt
    21901 ataagtactt ttgattcatt taaaaaaaat cactcgtatt cataactgta cggtattgac
    21961 tacgatttaa ttatgtagta aaatgaacat atagaaagta gtcataacta atggtcaaaa
    22021 gtaaggggtg tatttttatg atatttgggt attgtcgggt aagtagtaaa gtacaaattg
    22081 ataataatag tttagaacag caggagcagg aaataaaaag acattatagt gatgctaaaa
    22141 tatttaggga acaacataca gggtcaacca cgcacagacc tatttttgag gaaatggtta
    22201 atcaaatgcg tgatggtgat aaattagttg taactaaatt ggatagactt gcaagaaatg
    22261 caacagaagg cattaatctt atacaaaatt tatttgatag tggtatttct gttcatgtgc
    22321 ttaatgttgg gcttttggaa aatactactc ttggcaagtt ttttataaca accttgcttg
    22381 cagttgcaga aatggaaagg aatctaattt tagaacgaac ttttgcaggt aaggagatag
    22441 caaagcaaaa tcctaatttc cgggaaggta gacctaaaaa atatactaag atgcaaatgg
    22501 aacatgcttt agagttatta aaaactaatt cttatacgca agtagagaaa atgacaggga
    22561 ttagtaagag tacattgcaa agagcaaagc gaaaacaaga agactagata cctagtcttt
    22621 ttttattgaa aggagttgat ttgtatttca ataaaaaatt ccaatcgaga aaaagcgttt
    22681 gagatttaca aggagaaaaa tgggagaata aaattaattg atatctctaa gatgttagaa
    22741 gaaaaaagtt gcaatattag tcgttggaaa aaaattgata gatgggatta tagacttgga
    22801 ataaataaga aagttggcgc accagccggc aaccaaaatg ctttagggca tgaaggaggc
    22861 gcaccagccg gcaaccaaaa tgctagaact catggatttt tctctaagct tttgcctgct
    22921 agaacttacg agattgttaa atatatcgag aaggatggtg gcaattcttt agacattctt
    22981 tggaacagta tagtagttca gtatgcgaaa atattagatt cttttaagat tacccatgtc
    23041 aagaataaaa aagaccatac aactgacatt aagaaaaatg gtaataaaat taaagagtat
    23101 gaaattcaac attcttggga taaagttaca aatgctatga aggcagaggc gacagcattt
    23161 aaagctttgt caaaaatgat aaaagactat gaggaactac ttcataaaaa ttgggattta
    23221 gcaacagaag aacaaaaaag cagaatagaa aacataaaag cgagaacaaa taagttgact
    23281 ggtaatgact tagaaataga ggatatagaa gagatagagg cagaaatcta tggcagtaac
    23341 taagaagaaa actatacctt ttcaatttgg agataagcac aaagagtata ttaaaaagtg
    23401 cgctataaat acatacaata tagccgaagg cgctgtaaga gcgggtaaga cggttgacaa
    23461 tgtatttgcc tttgcgcatg agataaagac atctaaagat aagatacatc tagcaacagg
    23521 gtctacaagt gcaaatgcaa aattgaacat tggagatgct aatggttttg gacttgaata
    23581 tattttcaga gggcaatgtc attggggtaa atttaaaggc aatgaatgtt tgtatgtaaa
    23641 aggtatttca actaagcata agcaaaagat tgttattttt gctggaggtg ctaaggctga
    23701 tagttataag aaaataaggg gtaactcgta tggtatgtgg atagcaacag agataaatct
    23761 acatcatgac aatacaataa aagaggcttt taatagaatt atagcatcaa ctaatcgtaa
    23821 aatattttgg gatttgaacc ccgataatcc taattcaaca atttataaag agtatattga
    23881 cagctatatg aaaaaagata aagaagggac tttgctgggt ggatataact atcaacattt
    23941 tactatacat gataatataa ccgtatcaga ggaacgaaag caggcaatta tctcgcaata
    24001 tgatataaat agtatttggt ataagaggga tatattaggt caaagatgcg tagccgaagg
    24061 tcttatatat gaatactttg ctaataataa agaacagttt aagactgaaa cagttgaccc
    24121 gcttatggat gttattattg gagtggattt tgggggtaac aaatcatatc atgcttttgt
    24181 tgcgactgga attacttata attataaaaa agtaatagct cttgcaagtg agagacctaa
    24241 cgcagatacc agtccaaatg agttgaatgc tttacttata aattttataa aaaaagtaat
    24301 aaatctgcat ggtaaagtag attatattta ctgtgatagc gctgaacagg tactaataaa
    24361 aggcattaaa aatgcagtag aaaaagaaaa attaaatgtt agtgttagaa atgctttgaa
    24421 gagtgctatt aacgatagga taagacttac agacttatta attagtcaag gtaggtttga
    24481 gtatacaaag catagtgaaa ctcttgttaa tgctttatgt agtgctgttt gggatagtaa
    24541 agaaactaat gaggatgtaa gacttgatga tgggtcaagt gatatagata gtttagatgc
    24601 ttttgaatat acgatagaga gatacttgaa aaagtttata agaggtgact aaatgtttga
    24661 aggattgaaa aatgctataa ggggggtgat taacaagttg tttaataaga gttctataca
    24721 aaatgaatta aaggtagata tagctgttag tgataagatg tcaagagcaa ttgactcgtg
    24781 gatggctatg tacaaaaaca aagccccgtg gttaaatgaa acaactaaga gtttgaattt
    24841 gccaagtgca atatcaagtg aagttgcacg ccttgttaca ctagaacttg aaagtgaaat
    24901 agttggaaat gactttttaa atgaacaata tcaagaggta ttgaaagaca taagaaagta
    24961 ttgtgaatat gcgtgtgtgg ctggtggatt agtatttaaa ccgtatatag gcaggaataa
    25021 aatagaagtt gattatgtcc aggcaagtaa ttttttccct gttgaatata gttctacagg
    25081 tgatattaca tcagctattt tttctgaaat aaaagttaaa ggtgatatta aatataccag
    25141 attagaatat cataattgga cttctacttc ttatactatc tctaattacg catataagag
    25201 taatacagca agattaattg gttattcaaa tgacttaggg aagagaatta gattaagtga
    25261 tgttcccgaa tgggcagagc tttcagaaga gttagtatta gaaggaattg aaagaccttt
    25321 atttgcatac tttaaaatcc cgcaggctaa tagtttagaa aactcatcag ctctgggcgt
    25381 ttctgttttt tcaagagcag ttgacttaat aaaagaggct gataaacaat attcgaggat
    25441 tttgtgggag tatgaggcta cagagatagc aattgatgca gattctagta tgttcagaag
    25501 aaacgcaaat ggagaatatg aagttccgca tggcaagaaa cgactgtaca ggatgttaga
    25561 aatggaggat ggagacaata aatggaatgt gttttcacca gccataaggg attcgagtct
    25621 ttacgctggg cttaatcaat tactaagaaa aatagagttc aattgtggat tgtcatacgg
    25681 gattatatca gatgcacaag agattgaaaa aacagcaaca gaaataaaaa ctagtaagca
    25741 acgtttatac tctactgtta aggatataca aaagtcttta gaggatgccc tagagggatt
    25801 aatttattct atagataagt ggtctatttt ggcaggattt actcctagtt tgaagtatga
    25861 aacgactttt aattgggacg atagcgtaat aatagacaaa gattctgaac tcttagcaat
    25921 gcaacaggat gtcgcatctg gattaattag acccgaattg tatattatga aaaaatatgg
    25981 agtcacagag gaagaggctt tgaaaatgat gcccgacacg ggggaattag tagaggataa
    26041 tccttttgac aaggatggta tatagatgtt aacacctaat gagttacaag aaattcctaa
    26101 gtattttatt ggcttatttc aatctttaga ggactttata atcgcagata ttgcaagacg
    26161 tatagcaaaa gttgggaaca ttacagatat ggcagagtgg cagttaataa gagcagaaga
    26221 gattggaatt gctgataaag ttattaaaag gaaaattgct gaaacacttg gtatttcttt
    26281 tgagacagtt gacaagttat ttgaagaaag tgctataaaa tctattgaaa gtgatagtgc
    26341 tttatatgaa catgctaaat taacaccgat acacttgaat agttctgaag aacttaagaa
    26401 ctatgtttct tcagctaaag aacaaacgaa gggtaaactg aagaatatga caggtacatt
    26461 aggattttgc actattaaaa aaggtagagt tattagtaag aaacttacag acatatatat
    26521 agagtcttta gacttagcgc aatttcaagt cagtacagga gtcttggatt ataagacagc
    26581 agttaaaaaa gcagttaaga ggcttgcaga tagtggttta aggtttatag actatgagac
    26641 tggttggaca aatagaatag atgttgcaac tagaagggct gtgctgactg gtgttaatca
    26701 gatgtcacag aatattaaca ataaagttat agacgattta gatactgata tagtagaagt
    26761 tacagcgcac tcgggggcaa ggtgcgaggg tgcaggcatt aaaaatcata agaaatggca
    26821 gggcaaatgg tatagtttaa gtggtaaaag tacgaaatac ccttctttaa gggctgttac
    26881 aggctgggga cagggtgatg gattaggagg ttggaactgt tcgcatcaat ttcatgcagt
    26941 tgtacctggt atctctgttc ctgcttacac taaagagcaa ttaaaaaata tagaccctcc
    27001 cgatattgga tataaggggc gaacttatac gcattatcaa gcgttgcagt atcagcgcaa
    27061 aattgaaaca gctatgaggc agactaagag acaactaatt gcttacgagt ctgctggtct
    27121 taaagatgag tttacaaatg caagtataaa gttgcaaaga cagaagcaag aatatagaga
    27181 gtttagtaag gttgctaaat tgaggttgca gaatgataga caccaggtgt taggatataa
    27241 taagagcatc agccaaaagg ctgtttatgc tggcaaaaag gagaagtaaa atggatgttg
    27301 atatattagg tgctaaatat actatagtaa aagattgcac aaaagataaa gagcctttat
    27361 tggctaaatt tgatggtttt atggatgata ctgtaaataa gattgtaatt gctaaaatgg
    27421 agcattcaga tgaaagttta aaggatttaa acttttatga aaaacaagtt cttagacatg
    27481 aagttataca tgctttttta tctgaaagcg ggttaaagag taatagcgat tgggctagaa
    27541 acgaggaaat ggttgactat tttgcaatac aatttcctaa aatgttaaaa gtgtttatga
    27601 aattagacgc tttataaaaa aggagtgatt taaatgggga aatatagaaa gaaaccagtt
    27661 gttattgagg cttttaaatg gctaggaagt gcagaacaaa aggaagaacc gacatggata
    27721 gttagggcta ttgaaagtgg taatgtttgg attgaacaaa gcctagggga attatctcca
    27781 cgcatgtata taaggacact tgaaggtata cacgaggcta atgtaggtga ttacattatc
    27841 caaggaataa agggggaatt gtatccatgc aaggctgata tatttagaga aacatatgaa
    27901 gaagtcttat attaataagg cttattttta tgcttaaaaa tacttaattc atttatatag
    27961 atgtctacta agcgacgtaa aaagttagac aatatgtgat gctaccacgt aaaaagcgta
    28021 attgtaaacg aaagcaggag gtttttatga aaagagagtt tttgaaagat ttaaatttag
    28081 aggacgaggt tatagaaaaa atcatgtccg agaatgggag ggatatagaa aagtataaaa
    28141 aagaagtaga aaagaaaaaa gaagaattgg aaagtaaaaa tactgaactc gaaacagcta
    28201 ataataaaat taaagactta gaaaaaatag atgttgagag tataaaaaaa gaggttgacg
    28261 actggaagag taaagccgaa caggctcaaa aagataagga attgattgaa aatcaaatgt
    28321 cagagcagac ttataattta aacttagata attatttaag taatttcaag ttttctagca
    28381 atctctcaaa agaggctgta aaaataaaaa tgaaagaaaa aggacttgaa tataaggacg
    28441 gggcttttga gggagcagat gattatataa aagaattgca ggcgaatgac ccaggggctt
    28501 ttataagtgg caatagtgtt ccaaaggttg ttagttctag cagtggagat gctggagagt
    28561 ctaaagtaag tttaatggaa caaatgatag caaaaaatag agaaatgttg aacatatagg
    28621 aggtagaaaa tgagtttatt tgattcaaaa atttttaatg gagaagtgtt tggaaagtac
    28681 gttgagactg tacctaatct aaatagggac gaattaataa agtctggagc aataagaaga
    28741 aggggcgatt taaaggctat gttttcagcc caatcgggag caaattatgc aactattcca
    28801 atgcttggct tgattgatgg cgacccagtc aattacgacg gacaaacgga tatagtagca
    28861 actacaacaa agaccttttc gcagtctgta attgttgttg gtagagctaa atcgtgggcg
    28921 gaaaaagact ttagccagga cgtaaccggc ggagttcctt ttatggagaa tgtaggaaat
    28981 caagttgcta aatattggga taatatagac caaaaaactt tgttagctgt tttggaaggt
    29041 attttcgcaa tgacaggggc gaaaaattta gaatttgtta acaatcatac ttttgatata
    29101 acaaaagaaa cagaagcaga aaatcaaaag gttaaagctg aaactcttaa tagtgcaact
    29161 caaaaagcgt gtggagataa taaagaaaaa tttgcacttg ttataatgca cagtgttata
    29221 tcaactaact tagaaaattt aaatcttgtt gctaggttaa aatatactga cccacaaggt
    29281 attcaaagag aaatgaagtt aggaacttgg ggtggcaaat tagtcttaat tgatgacaat
    29341 atgccaacta gagaggtcgc tgaaagttca gagggttcaa atgatggata cacagagtat
    29401 acgacatatg tgcttgggga aaattctatt gacttcgagg atgttggtac aaaagtcaca
    29461 tatgaaatgg atagagttcc aggcaaaaat ggtggagaag attacttata tagtagacaa
    29521 agaaaagtat ttgcgccata tggtattagc tttactaaaa aagcagttgc aacagcatct
    29581 ccaactgatg cagaactgaa aaacggagca aattgggagc ttgtgaatga tagttcgaat
    29641 gggtctaaaa cttatataaa ccataaggct atacctatcg ctagaattat ttcgagaggg
    29701 taggctatga atatgtatat agactatgaa ttttatagca ctcttggggg agaaatccca
    29761 caggacaaat ttaataaata tgcaataaga gcaactaagt atatagatta taatactttt
    29821 aatagaatag aagaagttac agaagaaata aaaatagccg cttgtgagat tgctgacctc
    29881 atatataaaa gtaacctgga aggcgacaaa gaaattcagt ctgaaagtat tggaagtcat
    29941 tcagtgactt attcagctaa caataaaacg atagagcaaa aggcatatga tattttaaaa
    30001 atgtatttag attcagattt gttgtatcga ggtgtttagc gtgtttttta aagataatat
    30061 aactttatat aataaatatt atgataagta ctctgacaaa actttataca agagggctta
    30121 tttaattgga gtagattatc aaggttctaa aaatatagct gttacagata aggggttgtt
    30181 aagtgctgat agtgttaagg ttatagttcc cttttctgca aatgcaagag ggaagaaata
    30241 tatagaccct ttcaaatatt atgaattaga tgaaatcgaa aaggaaaaat tttatacttt
    30301 caaggttggc gatattattg taaaagaggt cgtagacttt gaaataacta gcgttaagcc
    30361 ttatacatta agagaattac aaagtaaatt tgatgatgtc agtattataa aatcagtcat
    30421 taaatgtgat tttgggagca taagaatgcg acatatagag ttggaggcag aatgatatgg
    30481 gatgaatgta cacgtagata ttgacgttgc tagaataatg agagagcggg gtcttgaaag
    30541 aggcgggaag gcacaaaaat tttttaccca tgaagttaga aggctttcag actcttatgt
    30601 ccctaaacaa agtggaacgt tgaaaaatac agctagagaa gaagttgaca agataaagta
    30661 tatacagcct tacgctaaaa tacattacta taataattct gggcgtggtt cagaaggtat
    30721 gggggcagga ggcaaaagag gcaagtattg ggataaaaga atgttagcag acaataaagt
    30781 tgagttgata gagtctgttg ctaggtttat tggaggtagt agagagtgac tatagttgaa
    30841 agcgttagag agtttataaa aaaatgcccc cacttagatg aatttgcaaa aagtataaat
    30901 gttgaatttt tagcggagga atttacttct tatacaattg agactgttcc agctgaaacg
    30961 attgtaaaaa aatttgtaaa tggtgatgca ataaaacaat ttgtttttat atttgcaagt
    31021 agagagtctt atgggtctga tgttatgcag aatatagaaa atagccagtt ttacgaacaa
    31081 tttgcggact ggatatatag agaaaattta aatggcaatt tacctgtttt agatggaaat
    31141 aaagagtcta tgtcactaga ggtaagtact acaggttatc cagttcaaac aagtatagac
    31201 acagcacaat atcaaataca attaaaacta aaatattttg aaaaaggaga aatataaaaa
    31261 tggcaggaat agaaacgata caaagatata aaatagcgga ttatttggga attttagggg
    31321 atgacggaca aagctcttgg gaattgatgg gagcaggatt taatacatta gacgagaacc
    31381 catccgcgca aagtgactct aagacatata taaacgatag aaacagtact agcactataa
    31441 aatcttacca aacgcaattc ccgtttgagt cagatttaat taaaagcgaa aaagctataa
    31501 tggaacttta taagataggt agagaccagg cgactggcgc ggatgccgaa agagattatt
    31561 taagagtaga attattttta ccagttgaag gaaaagataa tacttttaag gctagaaaat
    31621 ttagagtgtc agttgaagtc tctgggcttg caggagcagg aggagaaaca attattgtca
    31681 gcgggaactt aaatagcgtt ggtaattttg tagatggaga atttaacact cagacaaaga
    31741 ttttcacgga ggttggggaa gaagcaccac cagctgaaag tttaaaggtt ttaactgtaa
    31801 attcaattgc gggtactaac acaggggata caaaagtaac cgttactcca ggtcttgaaa
    31861 gtggaaatag ttataaatat aagactgggg caagtgtttc agttccagca ttagatgctg
    31921 attgttcagt tggatatact acttgggatg gtactgagga tataacagca aatgaagggg
    31981 ataaaatatt gatagtagag gttaattcgg gcaataaagc gaaaaaggca ggaatagcta
    32041 ctgttacagt aaaagagtag ctatataatt gaattaaaat atttatataa ataattaaaa
    32101 ataaggagat tcaaaagatg ttaattaata atatagaatt aaaagatttt gatatatatg
    32161 atgctgatga acatgaaaac attgaaaaaa caataggctt gactattact gaaacgggaa
    32221 aagttgagaa agaggcaacc aaaggttatg aaatgattag agatatatgt aatttaattt
    32281 ttaaatgctt tgacgatatt ttcggggaag gtacatcaaa taaaatgttt ggagataaga
    32341 gaaatttaaa ggtatgctca aatgcttttt ttgaattaat agaagaagtt aatagacaga
    32401 aacaagaggg ggaagaattt ttcgcctcag ctgtatcaaa gtatacgcca aaccgagcgc
    32461 aacgcagagc taaaaaataa tgaatatgtt gattgatgtg cttcccgaaa gcgttgaaat
    32521 tgatggggaa gaatataaaa ttaatacaga ttttagaata tcaattttgt ttgaaatgtt
    32581 gatacaagat aattctataa gcgacgagga aaaaggggaa aatgcccttc ttttatttta
    32641 ccctgtaatc cctaaaaata ctgcaacggc tatagataaa attatttggt tttattcgtg
    32701 tgggaaaaaa gaaaacaatg tggatgatgg ttgcagaggc ggtacagggt ctagtaaaag
    32761 tcaaatatat tcatatgact atgacgacga gtatatatat tcagctttct taggtcaata
    32821 tggaattgac ttacaggaca tagaaaaatt gcattggtgg aaatttaaag cattgtttaa
    32881 gtctttgaaa gaggacactg aaatagttaa aatcatgggc tatagggcaa tggatataag
    32941 cggagatatg ccaaaggctc aaaaagattt ttatagaaaa atgaaaaaga tacatgctat
    33001 accgcttcct caaaacgagg ttgaaaaaat aaacgaaatt gaaaaggcgt tgctaaatgg
    33061 tggagatatt agtaatttat tataaaataa tatactctac actttccgaa ttgatatata
    33121 attagtatat aaataatttt aattcggggg gggattatta tggggtttaa atttagaaaa
    33181 agtataaatc taggcggtgg attgaagtta aatattaata aaaatagtgt tagtgttagc
    33241 ggtggcgtta aaggtgctag agtttcagtt aattctaaag ggaaagctac gacgactttg
    33301 ggagttcctg gaagtggctt atattatcaa gaaacaactc aactaggtgg aggtaaaaag
    33361 aaaaatggta agagtgcagg aggctcggag agtagtaggc aggctcaaca gctattaaaa
    33421 ataataaacg attgtgctaa tatagttaat actacaaaag accctaaaac atttttttat
    33481 agatataata tgttgttaga taaatcatat gccttagttg ccatagagga taacttaaac
    33541 ttttctggtc aatctcctag ccagatgcta gacagcataa tagcaaaaaa gactgatact
    33601 ataaatgact ttttgactag atactacaat gaaatgtcta gtaaattgaa agaactaaaa
    33661 actgaaaagg ctcgcatgaa caatgcttat aaattctcta gtttagcaat agattataaa
    33721 gagcatttaa ataatgataa taaaactaag cttaataggc tatataaaca actattaaat
    33781 gatgttaaaa aacaagatta atatttgtaa agagaaaaat atagtagaag atttaaataa
    33841 aaagcactta cttttttaaa aagtaggtgc tttttaatgc aaatttataa aaagggggtg
    33901 aatagatggc agatggttct attattatag ataccttttt aaataccgaa gatgcagaga
    33961 aacaacttaa taacttagct gatactatga agaagaaagc aaaaagcgct cttgcgatag
    34021 ctggaattgg cgcaagtctg ggagctatag gaaaacaggc tattgacttt ggggatgaat
    34081 accaaaaggc aatgaatggt ttcgagtcag caacgggaaa tgcggaagct aaggcaaaag
    34141 gctttggaga agcgctacag caggtctatg caaataattt tggcgaggac atgggcgata
    34201 taagcgaagt tatgagttta atatctcaaa agcttgatgg gatagacgct agcaatattc
    34261 aagaagttac tgaaagtgcg attgtgatgc gggatacttt cgacatggat gttggggaaa
    34321 gtttaaatgc cgtaaactcc atgatgcgcc aatttggtat tagcgcaaaa gagtcgtaca
    34381 acttgattgc acagggagca caacaaggat taaatcagaa tgatgacctc ggagaccagt
    34441 tagcagaata ttcgacatat tacgcacaga tgggtttttc agcagaagaa atgtttaata
    34501 tgatgaaaaa cggggcagag agtggagtct atcaaataga ctatctaaat gacgctttga
    34561 aagaatttaa tattagggct aaagacggtt cgaatggaac taaggaagct tttgaggcgc
    34621 tcggttttaa tgctgacgaa ctaactaaga aatttgcaaa tggtggaagt agtgcaaaag
    34681 aggcttttgt tgaggttaca acagctttaa ataatttaga tgataatgta ttaaaaaatc
    34741 agataggtgt acaactcttt ggtactaagt ttgaggattt ggaagcggat gcagtaacag
    34801 cgctaacaaa tatcgaagga agtataagca gttcaaaaga taaattagaa gaaattaata
    34861 agataaaata caatagtttc ggagaagcga taacaggaat tggaaggcaa atgcaggtta
    34921 atttacttct tccaattgca gagggattgc ttcctgtttt aaatgatttg gcaaataaat
    34981 ttgctaaggc ttttcaaaac gaaaatacaa aaaatgctat aagaagtaca gcagaaagca
    35041 ttggtgcgtt aattggaaca gttgtaaagt taatatcttt agcattaacc cctttaatca
    35101 atgttataac cttcttgatt cagcatggta gccttgtagc tcctttgata acagggattg
    35161 gagttgcttt cgctggatta aaaattgctg gaattgcaag gggtatacaa aaaagcttta
    35221 cagatgcgca acttgctgta aggctgttcg tcacagggat ggcagaatca ggaactacgt
    35281 taacgctgta tgaaactata gttggattag ttacaaaaaa aataagttta gcgcaagtag
    35341 caactaattt gtggaagggt gcattaacgg cgcttggtgg tccgattggt ttaactatag
    35401 tagcagtagg ggctttgatt gcggtattcg tatcgctctg gaaaaacaat gagggtttca
    35461 gagatgccgt tataaatatt tggaataata ttaaagaagt tggggagaaa atattcggtc
    35521 aaattgctaa attctttact gaaacaatac cgcaagctat gagtaaattt atagactttg
    35581 caaaagaaaa ttggcaagga ttggctctat tacttgttaa tcccttcatt ggtggtttta
    35641 aattaatcta tgataattgc gaaggtttta gaaatactat aaataaatta tttaacagca
    35701 ttaaagatgc tttaagcaaa acaatgtctt ttattaagag taactggcaa ggcttattat
    35761 taattttagt aaatccgttc attggagcat ttaaattaat ttacgataat tgtgaaggct
    35821 ttagaaacac tgtaaattct atatttaaca aaattgtagg catctttaaa aatgttttaa
    35881 gttttattaa aaataactgg aaaagtatag gaagtatgtt attaagccct tttgtaaatg
    35941 gatttaaagc tatttataat agttgtgtaa gttttaaaaa taaggttttt agtttctttt
    36001 ctagtgttgt aaaaggcttt gttacctttg gaaaaaacat aatagctggg ataataaatg
    36061 ggctttcgag cggtatcggt ttggttgtag atactgtaaa aaatatcggt ttaactgtag
    36121 ttaatacatt taaaaaagta ctaagaatac attcaccgtc taaagttaca acagaacttg
    36181 gagaatttgc aggcattgga tttgctaatg gtataaaaaa tacaagcaaa gatgtagtag
    36241 aggcaacaaa agaattaata agtgctatgg aaaaaaatct tgagaccaac aagagtaaat
    36301 ttgaaaaaat ttgcgaggct ataactacag cattaaaaaa tcaatatgaa agtcaaaaag
    36361 atatacaaat aaaagcgctt gatgaaagat taaaagttga ggaaaaggca tcgaatgata
    36421 gattaaaggt ctatgaaaaa gaatacaatg aaaaattaaa atatttagat acagaaacaa
    36481 acgaaaaaac taatgctata caagaacaaa ttgacgctat agacaagcaa attgaagaag
    36541 aacaaaaagc agaagaagag aaggcgtaca atgaaaaaat aagcgacctc aataggaaac
    36601 tagcaacagc taaaaatcaa aaagaacgag aaaaaataca aaaagaaata gcagaaacac
    36661 aagcagatag acaaaagaaa ttattagatg aacaaaggca acaagaaaaa aacagattaa
    36721 aagaacagat tgaaaatata aaaacagaag ctagtaacaa aaaagagcag tacaaagaag
    36781 agtacgacaa tcaaaaggaa gcagaaagta aaaaacttga attaataaaa gagtctaatg
    36841 ctagtcaaaa agaagaaatt gaaaaatatt ttagtgaact tttagaagag acaaatattc
    36901 aaaatgaagc gagaaggcta cttttacaaa aaaattcaga ggaaattata aaacttctaa
    36961 gcgagtacaa tccgcattgg caggacgcgg gacaaagttt agcagatagt ctattaaatg
    37021 gagttaactc taaaaagcag tctatacagg aagctgtaaa agaagctatt aatttaaaag
    37081 agattatacc agcacaagag caagaactcg atagattaaa gaaaaaatta gaagagtatg
    37141 agaagctaaa agaaaatgtt agcgcatcta gcgctggtga tggagatttg gacacatccg
    37201 ttcaagattc ttcattagat tcgggttcta gtgggctgga ttctaaggaa cttgaagaat
    37261 atgcaaatgc tgtagatgat gtaaaaactt cggttgaggg tttagacctt gtaagcgatg
    37321 aactggtcaa gggaacagtc ccaaaagttg gagattcagc tggaaaatta actgaaagca
    37381 taaaaaaagg gtttggtagt attggtggat tttttggtgg aattgagaaa tggctaaggg
    37441 acacagataa aagtataaat gactggcttt ctagtgcatc aagttccatt tcggactttt
    37501 tttctaagct aaaagataaa tttaacgcag gaattgaaaa tattggcgga ttttttagtg
    37561 gtcttggtgg caagatagga gatagcttta acggattagg cgactttttt aatggtttgg
    37621 aagataaggc aaaagaaggc ttttcaagta taaaaaataa tgctatagaa gtctttagtg
    37681 ggcttggaga ctggtttaaa gaaactgggt caaaaatagg ggatgcaatt ggaactggtc
    37741 taaaaagtgg tttggatttc tttactaaca cagtacccga gtggtttgca gggcttggcg
    37801 ataaaataaa agaagaatgc gggaaaatag ggggaaaaat atcaaacttt tttaatgaaa
    37861 ccatgccaac cataattagc aacatagtag aatggtttaa gcaaattccg tataacatcg
    37921 gatttacaat agggtctgtt gctggttttt ttgttgattt aggagctaaa ttatacgcat
    37981 gggcaacaga aacattaccc gaaataatta atagtatagt ggaatggttc aaaagtttac
    38041 ccgaaaaaat cggggaattt cttaatatta cactacaaaa tattcagacc tggggtgaaa
    38101 atttcaaggt ttctgtatct gaatttttta gtgtaatttg ggaaagcata acacagtttt
    38161 tcacagagtt acctgaaaaa atttcggaat ggtttggcac ggtagtagag acagtaagtg
    38221 gctggggcga gaatttaaag caactagcaa cagatatttt taataacttt gtagaaaata
    38281 tattaattcc agtatcgcaa ttaccaggaa aattgtggga aaagtttacg gaagttattg
    38341 ggaaagtgac tgaatggggt tctaatctat tgcaaaaagg gatagatata gcgagcaaat
    38401 ttttagagaa tgtaatgaaa ttcttttcag aacttccagg caaaataaaa gaaaaattag
    38461 atgatataat aaaaaaagtt acagactggg gaactaattt agtaagcaaa gcgctcgaaa
    38521 tcgcaaagaa cttttttaat aatatataca atactgttag tcaactacca ggtaagttta
    38581 aagaatggtt ggacagcatt attaataatg tcataagctg gggaactaac ttagtcaagc
    38641 gagccgaaga agctgggaaa aacatggcaa atgccgttaa agatgcttta aaagatttac
    38701 caagcaaaat catgagtatt ggaaaagacg ttgtaagggg cttatgggaa ggtattactg
    38761 gaatgggcgg ttggctaaaa ggcaaggttt tcgatttcgc tggagatatt ataaatggat
    38821 ttaaagatgg atttggagtt cactcgcctt ccataatcat gcgtgatttg attggtagaa
    38881 accttgtaaa aggggttggt gttgggatag atgtagaaac accagagcta aaagaaaaaa
    38941 tagagaaaaa tatatctgaa cttactagta aattaaaagc tacagttaac tttgagacat
    39001 ctaaaataca agctaatata gttgcaagta cggattttaa agctggaaaa gaaacagcta
    39061 taatgagtag taataaagat agcgaagtta actcaaatca agctggaata aaacttaaca
    39121 tagaaaactt tgtaaacaat agagagcagg acatagaaaa cctttttaat gaaatactgt
    39181 tcctagcaaa aagaaaaggg gttatataaa agaccctctt ttctttttat ctttctttta
    39241 aacactatag taaaaatgag ggggtgaaaa ttatattttt tatttatgat ggtcgggata
    39301 gtagggaatt tggtttaaaa atatacaata taaatgacct atcagcccca caaatagaaa
    39361 ttgaaaaagt gaatgtgcca ggcaaaaatg gcgatttatt actagagaaa ggattcggaa
    39421 actttgcttt aacaatagaa tgcgacatag atgcaaggca aagcaatata gaagaagttg
    39481 caacagaaat aaaaaaatgg ttgcaaggtg atatatcata taaaaaactt tttttaagta
    39541 atagcgactt ttattaccta gctagttgca acaataagct agatataatt agaacattta
    39601 aaaactttgc atcatgtctt ttaacattcg actgttaccc attcaaatat gcagaagaag
    39661 aaattataag cttgaatgta ttaaatctaa agagtactac tataacaaat ttttatagag
    39721 aatcaaatcc tgttttttat atagaagcga caggagacat aaatataaaa ataaatactc
    39781 aaaacattga gttaagaggg ataacagaga atggaatttt aagcgactta ataatagatt
    39841 cggaaatcat gaatgtttac agagaaaata aaaacacaaa tataatagtc aatgaaaatt
    39901 ctaaactatt ttctgatttt ccaaaacttg aagaagggga aaatcgaatt agttgggaag
    39961 gagatataaa aagtataaaa ataaatccta gatggaatat tttataaagc ttttatattg
    40021 aaaatggaag ggagggatta atattcaaaa agggaaatta attttatatg aagaaaaaga
    40081 aatagatttt agacatttag ggctgggtgt tcttacaaat gtaataaatg accatgtcag
    40141 agaggaagag aacggagttt tcgagttaga atttactatc tacgaaaact cttttctatt
    40201 taaagaaata aaaactgata gattagtaaa agctgacgca tctccaaatt ttaaagggca
    40261 actttttaga atttattata tatcaaaaaa tttaggtgga tatattaatg taaaagcaca
    40321 gcatgttaaa tatgatttgc ttaataattt catagaaagc ttagaattga atgatattac
    40381 ttgtgaagaa gctttagaaa gagtctttcg tgcttgcgag gaacagaata gatttagagg
    40441 gcattcagat atcaaggcaa ggaatactat taatattgaa atgcaaagcc cttacagcgc
    40501 aatctgtgag ggcgaaaatt ctctaattca aaaatttgaa ccaactgcaa aacttttctt
    40561 tgataatttc gacgcttatt taaattatca aagaggagaa agtaaaaatg tattacttgc
    40621 atatagaaaa aatataacag ggcttgaagc ggaatatgat acacaagata ttgtaactaa
    40681 aatatatcct tttgcaactt acgaggatga aatgattaca ttaacagaaa aatacattgt
    40741 tagtcctaat ctgaataaat atgctactca aaagattgtt gctatagatt ttagttctga
    40801 tgaggtcagc gttgaagaag aactaagaga aaaatgcaaa gattatttta aatacaatca
    40861 agttgattta ccaaaagttt tatataaagt caactttgta gatttgtcta caactgtaaa
    40921 ttataaagat tataagatgc tagaaacagt caatctaggc gatgaagtaa taatacgaga
    40981 ttttaattta aacatcaatg caactgctag agtagttaaa acagactata gcccaat
//
LOCUS       DBGJFF010000024        38917 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.024, whole genome shotgun sequence.
ACCESSION   DBGJFF010000024 DBGJFF010000000
VERSION     DBGJFF010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 38917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38917
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.024"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            247..603
                     /locus_tag="ACOIAF_001280"
     CDS             247..603
                     /locus_tag="ACOIAF_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668299.1"
                     /translation="MKRPLAYLTAAWSGEPDVDMELAAHYCRLAYEAGFSPICPLLYL
                     PLFLNDSVPEEHKAGIDMRRDMLRRSHTLIVCGSVVDEDVKNDIAVAGRLGIAATTLE
                     GVLAVKGHGIPGHAGH"
     gene            590..1675
                     /locus_tag="ACOIAF_001281"
     CDS             590..1675
                     /locus_tag="ACOIAF_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005927707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HGZ2668300.1"
                     /translation="MPDIKLGSLFDGIGVFPLAASRCGIRPVWASEIEKAPISITKRH
                     FPDMVHLGDITKVDGGKIPPVHVITFGSPCQNLSLIGNRSGLAGAKSSLFYQAFRIIQ
                     EMRDATDNLYPAIAVWENVMGAFSTNDRMDFRAVLSAFSDTEVPMPPSGRWGNAGMVR
                     GGTPDVCWRLMDAQYWAGTRRLARRQRIFVVADFGGRRAADILFKPRPMLPLPPPCGE
                     GGRAAAEGDRTASFETGRQIPVIHPFQCFRMRGAAKRQEETAFQNSFGLPTDPFPTLL
                     ASDVTPFAFWYEGDPEGGCIRFLTETESERLMGLPEGWTKYGADGVEIRPLQRYKALG
                     NAIALPCADYIMAGIYEVLADRAGKEE"
     gene            1680..2393
                     /locus_tag="ACOIAF_001282"
     CDS             1680..2393
                     /locus_tag="ACOIAF_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005927704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HGZ2668301.1"
                     /translation="MFEAYITNTALYPLMGIEVGTTVHFPMTTQELQAALAKIGIDGK
                     RYSEVFFISFDSDVLGLYDHLYECEDIDELNELGHALLEVRDKGGLETFEAALVLGNH
                     TRSVKDLINLTQNLDLYRFYPDISDDEGLGRLYADELGTIDIPEHIQNYFDYEAYGRD
                     VRINEGGVFAPGGYVSAVPEGFKEYYHGPQDIPPEHRIFAYPEKAEPVHSILAALKRF
                     QEAPPAPKKDKAGPSHEER"
     gene            2420..3457
                     /locus_tag="ACOIAF_001283"
     CDS             2420..3457
                     /locus_tag="ACOIAF_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668302.1"
                     /translation="MKEVHTINYYPINEGTARRAKEMNSFSDYKEGSATAEYRAMVDK
                     AAAIAEKQKSRVDPMYHEKIDHLLDTYARKLAENMNQGFAIDARVPSMMIAGPANFPV
                     GKKEKQNRARDSNMEEWRYIQGLLDKIRSTGMGGISADDPAAIEKLQKKLDGLERSQL
                     IMKEVNAYYRKHGKLDGCALLSPDQIEKLKASMASSWRSDPRPFESYQLTNNNAEIRR
                     VKARIEQLSKQAQREFSGWEFDGGRVEMNREDNRLQVFFDGKPDADTRAELKSSGFRW
                     APSVGAWQRQLTDNAIRAADRLECIKPLSGEKPSQLQKKPSILQTMREQGEKVQTEPE
                     KKAPSGRDAER"
     gene            3549..4019
                     /locus_tag="ACOIAF_001284"
     CDS             3549..4019
                     /locus_tag="ACOIAF_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007887898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HGZ2668303.1"
                     /translation="MQEEVNQKTVALSIRTTKLTGKVLAAALGKVARALQKHHRKALT
                     PQGRQSVKKLMNHYGTKSAMPYVGAPKDFDRIAKEFHVDYAFHKVSPGHYLLFFKANQ
                     ADAITAAFQKYSAKVLNKEQDKASILGQLRKFTEQIRTQAKEKQRTREAVKDGR"
     gene            4016..4636
                     /locus_tag="ACOIAF_001285"
                     /pseudo
     CDS             4016..4636
                     /locus_tag="ACOIAF_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901619.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
     gene            5330..7198
                     /gene="ltrA"
                     /locus_tag="ACOIAF_001286"
     CDS             5330..7198
                     /gene="ltrA"
                     /locus_tag="ACOIAF_001286"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HGZ2668304.1"
                     /translation="MTQKAKKRSKLRHAEYYDMQNIFDELYAKSKDGEIFNNLVEIIA
                     APNNIMLAFRNIKSNDGSHTAGTDGRTIESLADMSEEDFVSLIQKQFTRYVPKTVRRV
                     EIPKPNGKFRPLGIPCIIDRIVQQCILQVMEPICEAKFYEHSYGFRPCRSAENAVSYA
                     YGLAQRNKLHYVVDVDIKGFFDNVNHRKLLQQIWTLGIRDTKLIQIIKAMLKAPIQMP
                     DGEILYPDRGTPQGGILSPLLANIVLNELDWWIASQWEGMADHMKSPCKVTYYPNGAE
                     KKCNSYTALKKSNLKEMRIVRYADDFKIFCRNRKDADRAYHAVKDWLLKRLKLEISDE
                     KSKVTNLRKRDSEFLGFRMKLIRKRRTWVISSNVCDKAMSHMQKELSKAIIEIQNSKT
                     ASNIKSNIHEYNAKVIGMQNYYCIATRINLNFSRMAHVNNGRFLHRIDGYKKQGELNN
                     KYLKNRYGKSKQMRWIGDTPVVPLAYVQFKIPMRKSRKINPYTPDGRMEIHSNLKLNV
                     EDMLWLMRHPVIGETVQFNDNRISLYAGQEGKCAITGEQLEVIHCICYRKSNECKNGR
                     DSYRNLLLLSPAGYEFISTTDSIKFATLTKKYELNKAQITKVNKLRVTAGLAEMSM"
     gene            7351..8553
                     /locus_tag="ACOIAF_001287"
                     /pseudo
     CDS             7351..8553
                     /locus_tag="ACOIAF_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901619.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
     gene            8643..8858
                     /locus_tag="ACOIAF_001288"
     CDS             8643..8858
                     /locus_tag="ACOIAF_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002575635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family mobile element protein"
                     /protein_id="HGZ2668305.1"
                     /translation="MEFFNQAIDILKILVMALGAGLAVWGVINLLEGYGSDNPAAKSQ
                     GIKQLMAGGGVVLIGLQLIPLLSGLFS"
     gene            8900..9766
                     /locus_tag="ACOIAF_001289"
     CDS             8900..9766
                     /locus_tag="ACOIAF_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB6/TrbL-like conjugal transfer protein,
                     CD1112 family"
                     /protein_id="HGZ2668306.1"
                     /translation="MDFIIDAIVEWLKGLLVDGIMGNLDGLFDNVNQSVGEIATQVGT
                     TPADWNAGVFSMIRQISETAILPIAGVILTFVMTYELIQMLIERNNLHEVDTWMFFKW
                     VFKTFVAVMILTNTFNIVLAVFDVSQHVIQQSAGIIQNGTEITPDVLDSLRTELEVME
                     LGPLFGLWLQSFLIQLTMIALNIVIFVIVYGRMIEIYLLTSLAPIPFATVPNRETGHM
                     GQNYFRSLFAVGFQGLLILVCVAIYAVLIQSIATGGDPIGAIWGCVGYTVLLCFTLFK
                     TGSLAKSIFGCH"
     gene            9808..10347
                     /locus_tag="ACOIAF_001290"
     CDS             9808..10347
                     /locus_tag="ACOIAF_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005361089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HGZ2668307.1"
                     /translation="MPKEKFSIIYADPPWRYEQKNRQGSAELHYPTMSIEEICSLPVA
                     DLAAKDSVLFLWATFPQLPEALKVIKAWGFSYRSVAFVWIKRNRRSYSWFYGLGYWTR
                     GNAEICLLAVKGHPKRKSAGVHQLIVSPIERHSKKPDEARRKIVELMGDIPRVELFAR
                     EKKPGWDVWGNEVESTVNL"
     gene            10390..10812
                     /locus_tag="ACOIAF_001291"
     CDS             10390..10812
                     /locus_tag="ACOIAF_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein SpoVG family protein"
                     /protein_id="HGZ2668308.1"
                     /translation="MQEKTVGGTPAAPIKLDVSVRVIEPVKNLMGFASVKFNDCFVVE
                     NLKIVQGSKGLFLGMPSQPDGKGGYRDMAYPVTKEFREQLNTAVLQAYEAKLEQMAER
                     GSVGRASISDQLKAGKAAAEQAKAERPPKEKPSRSAER"
     gene            10855..11475
                     /locus_tag="ACOIAF_001292"
     CDS             10855..11475
                     /locus_tag="ACOIAF_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668309.1"
                     /translation="MPRKRMGYDAACYYDGKLLGRCTKADSDAYTLLMNACGGDAARV
                     LREYAYFSPELRAILEKAALMQADRNRTGGMFHAPKSSPWGEVQSCETLCPGVFLVST
                     ASHGGTMVANEVAAVLSPAAKKCGFKDKGYICYEEDAQESVVLRELLDKKLWNIPDRI
                     KDKGQFEENLNQSIRQYHPEYWRARQSGREAAEAARSTAPAKEAAR"
     gene            11520..11906
                     /locus_tag="ACOIAF_001293"
     CDS             11520..11906
                     /locus_tag="ACOIAF_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HGZ2668310.1"
                     /translation="MAYVPVPKDLSKIKTKLAFNLTKRQLVCFSGAAAVGLPAYLLSR
                     GSIGNSAAMFLMIGLMLPFFFLAMYERDGLPLEKVLKNIIRTRFLYPRVRPYKTENFY
                     ALLSARKEAQPIAKQQKGRKARRQKA"
     gene            11860..14010
                     /locus_tag="ACOIAF_001294"
     CDS             11860..14010
                     /locus_tag="ACOIAF_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4-like conjugal transfer ATPase, CD1110
                     family"
                     /protein_id="HGZ2668311.1"
                     /translation="MRNSRRAGKPAGRKPEKQGLTGLFTKGKNIPTTAQQTLPYREMY
                     RDGVCRVADHYYTKTIEYEDINYQLAQSEDQAAIFDGWSACLNYFDSSLPFQLSFLNH
                     RSRPGSRYSVNIPMQDDDYNSVRCEYVEMLENQIAKSNNGIVRTKLLTFGVNVDDLPT
                     ARARLERVEADICGNFKKLGVKCRSLSGLERLELLHGQLHPDSGSPFRFSWDMIPKTG
                     LSTKDFIAPDSFDFRFSRLFRVGTTWGAASYLQILASELSDKLLAELLEMDAEMTITL
                     HIQTVDQAAAVKSIKAKVSDIDKMKVEEQKKAARSGYDMDILPPDLVTYSNDAKTLLE
                     DLQSRNERMFLLTFLVVNMAPTRRELDNDLFTVSGIVQKYNCTLKRLDFQQEDGFLSS
                     LPLGHNGIEIKRGMTTSSTAIFVPFMTQELRMDGEAVYYGLNALSHNVIMANRKKLKN
                     PNGLFLGVPGSGKSFAAKRELVNVFLATKDRIIVVDPMGEYSPLIRRLGGQVIEIAPD
                     SPHHINPMDIDLSFDEENPMALKADFILSLMELIVGGKDGLQPVERTVIDRCVRQMYR
                     EHLQDPETSKMPTLQTLYDLLCSQPEGEAVRLATALEIYVSGSLNVFNHETNVDLNRR
                     LVCLDLKKLGAGLRTIAMLIMQDLVNSQVSMNFLRGIATWCYFDEFHVLLRDRLTASY
                     CVAIWKMLRKKGCVPSALTQNVKVRPDRALCKAV"
     gene            14504..16294
                     /locus_tag="ACOIAF_001295"
     CDS             14504..16294
                     /locus_tag="ACOIAF_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HGZ2668312.1"
                     /translation="MKPTSEILERIEKCSSEHKDGVFTRLYRYLLREDIYYAAYQKLY
                     ANKGATTQGIDDDTADGFSDFYIKELIQSLKDGTYKANPVRREYIPKKNGKLRPLGIP
                     SFRDKLLQEVVRMILEAIYEPVFDSHSHGFRPGKSCHTALRQISSDFTGVVWFIEGDI
                     QGCFDNINHEKLIEILSRKIKDSRFLNIIRQFLKAGYIENWKYNATYSGSPQGGICSP
                     ILANIYLNELDKKFREIAELFDKPRSAYQTLEYHAASKELKRLSYWIDHTSDEAARQE
                     LIDQHRAQKKAMRNLPCKPADNKKFTFVRYADDWLAGVCGTKTECEELKAEIAEFLST
                     ELKLTLSEEKTLITHSSEKVRFIGYDICVRRNQEVKGHRMKNGTWRKSRTLHMKVALS
                     IPHTEKIEKFMFAKKVIRQKENGEFQPIHRAGLLNLADYEIVEQYNAEARGLCNYYNL
                     ACDYHTLDYFCYLMEYSCLKTIANKHKTSIRKIIRQYKDGKTWSVPYETKTGTKRVRP
                     VKIADCKRGEASDIIYQRKKFSWKTTIRQRLNARVCELCGCKEADLYEVHVIRNLNEL
                     GNSDWETVMKKKRRKTLVVCSKCHERIHRH"
     gene            16412..16690
                     /locus_tag="ACOIAF_001296"
                     /pseudo
     CDS             16412..16690
                     /locus_tag="ACOIAF_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901641.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4-like conjugal transfer ATPase"
     gene            16940..18607
                     /locus_tag="ACOIAF_001297"
     CDS             16940..18607
                     /locus_tag="ACOIAF_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1108 family mobile element protein"
                     /protein_id="HGZ2668313.1"
                     /translation="MRQEDEAGGPQDTGKAQEPEGSAEKAGSKKDKYQKAQAKAERAG
                     EKLGKAREKLDKVEAKRAAQKPPGLAKKAVRGARTEAWFYVHNKIHEVEHENVGVEGA
                     HKSELVAEAGARKLTRYAKRRWQEHPARKVAKWERKDIKARANVDFQKMASEHPELAS
                     NPLSRVQQKWKLKRRYSKEAKAAAKQGAKAAKKTAAASGTATRRAAQFVTRHPVAVLI
                     LLLLLLLCFLVSAVSSIFPTLGSGLANALSSTSYASEDTDLLGVDEDYTALENELAQT
                     VANIESTHPGYDEYRYSVDEIGHNPYELASYFSAKYHVYFREQVQDELREIFEAQYEL
                     TLTEEVEIRYRTETSTDPETGETTTEEVSYEYYILNVTLTNKTLPAVILPRLNEQQRE
                     IYTVMQQLKGNKPYLWDGIYNGGEDTGPNYEIPGEALDDPAFAALMEEATKYIGWPYV
                     WGGSSPSTSFDCSGFVCWVYTTSGIHNLPRTTAQGIYNQCAIISPSEAKPGDIIFFTG
                     TYDSPGPVSHVGIYVGDGMMLHCGSPIQYANINSSYWQTHFYAFGRL"
     gene            18630..18896
                     /locus_tag="ACOIAF_001298"
     CDS             18630..18896
                     /locus_tag="ACOIAF_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006574806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HGZ2668314.1"
                     /translation="MNKIDKLDKELEKAREKAAEWQAKIRELEKQKQEEENSQIVQAV
                     RSLKLTPAQLMAFLNDPKNSLTASGHTDPKPEVPGKEDTAHEEN"
     gene            18883..19530
                     /locus_tag="ACOIAF_001299"
     CDS             18883..19530
                     /locus_tag="ACOIAF_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015567062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HGZ2668315.1"
                     /translation="MKKIKYRRLAAMAASLLCCLIFTVPAYAQSSEPQPETAPSPAET
                     EAEPETQNPFTPDGTGTVVDNATDEDGKEFYTITTADESVFYLVIDKQKTSENVYFLN
                     TVTTDDLLPLAEQGKEPPKEVTPEPEPKPTEPVEEVPEPEPEKKDSPLLSLLLIGAVV
                     LAGGGIGYYFKIYKPKHEAPDLEDDYCEYEDGELEEIAEEPEDEDTPPWEEDTEE"
     gene            19725..20126
                     /locus_tag="ACOIAF_001300"
     CDS             19725..20126
                     /locus_tag="ACOIAF_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015567060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP protein"
                     /protein_id="HGZ2668316.1"
                     /translation="MDARELTRDEKKKIRTLVTGMCANYDRESGLCLPLDCACYMLHK
                     CWTGAYCRYFREAVLPLDPELQAALTTEGISPELRACAVCGKAFWPEGRQAYCSDACE
                     AEGNRRKSRERMRKMREKRPGGGYDLPPPKA"
     gene            20259..20849
                     /locus_tag="ACOIAF_001301"
     CDS             20259..20849
                     /locus_tag="ACOIAF_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668317.1"
                     /translation="METVQGYVILKAATFETGHGFALGHNPGAPSPFVTWQFTEGENG
                     HRDYYWGRYGTSQAWAQRDFDRRVDDYQQLYHAAVKHTELGPEGVYRYYSTQRPVDIG
                     TYPKLPDNQPLSIVNYDDDRRRPVADSRLMAWGELTYAKPLTEKQMEDYELKPAPGNP
                     DRVRPSITARLKEGTRGQEPPKEPGPKRSHKTREER"
     gene            20862..21044
                     /locus_tag="ACOIAF_001302"
     CDS             20862..21044
                     /locus_tag="ACOIAF_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003500078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4316 domain-containing protein"
                     /protein_id="HGZ2668318.1"
                     /translation="MHEKDNYLKTAELSTEQNCNMIDGVPNNTPIQPTPPELDAKPLD
                     KVKEPKERRKGRELER"
     gene            21044..21385
                     /gene="mobC"
                     /locus_tag="ACOIAF_001303"
     CDS             21044..21385
                     /gene="mobC"
                     /locus_tag="ACOIAF_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HGZ2668319.1"
                     /translation="MENRKRNVHLHVMVTPDELAAIHERMAEAGISNAGAYVRKMALN
                     GYILHIDLAPVKELISLQRRCSNNLNQVAIHAHTYGVYPEEIDGLKRDYEKLWGEVSK
                     VLRELSELVAK"
     gene            21561..21851
                     /locus_tag="ACOIAF_001304"
     CDS             21561..21851
                     /locus_tag="ACOIAF_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005927606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1845 family protein"
                     /protein_id="HGZ2668320.1"
                     /translation="MKILLKILIAPFALALSLLAALLVFLFDICAVLLTIASVILAVL
                     GVALFFTPTPIGGIVFLFLAFLLSPYGLQAAAGSLLWALDGGKSALYRFLAS"
     gene            21931..23346
                     /locus_tag="ACOIAF_001305"
     CDS             21931..23346
                     /locus_tag="ACOIAF_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008401432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HGZ2668321.1"
                     /translation="MATTTLLQRHAGEGETIAEAIRDCLDYGKDPEKTESGKYISAYE
                     CDPATVADEFLLAKASYAAMTGREQKKENNVLCYQIRQSFYPGEITPKEANRIGYELA
                     MRWTKGRHAFIVTTHTDKQHIHCHIYYNSTTLDCTRKFRNFWGSSFALRRLSDRLCLE
                     NGLSIVENPKPRSKGKYRNYGEWQKERKGPLSYQDRLRLAIDTVLAEHPADLDEFLNL
                     MKRAGYEVKTVRGGGISFRLTGQGQERFTRLRASTLGDGYDLQDVLAAIEGKEKRPGH
                     SERKISLAVDIQAKLAAGKGPGYERWAKVFNIKQMAAALAYIQDNGLTDYEQLAQKAT
                     EAADRFHAISEQIKQTEQAMKTNAGLKAATVQYAKTRPVFEQYKATKYSRKFLTEHEA
                     DLELYRAAQAEMRSLLGGAKLPKMDVLKVEGRKLTAKKKQLYGEYQKARRDMQEIVTI
                     KANIDTLLGYTEPGRKHEKER"
     gene            complement(23516..23881)
                     /locus_tag="ACOIAF_001306"
     CDS             complement(23516..23881)
                     /locus_tag="ACOIAF_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005930876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGZ2668322.1"
                     /translation="MRMNQDERRFDFHGLGAALKRAREEKGWTQAYVAELVDRDSRTI
                     MNIENKGQYPSFDLFVKLITMFDVSVDQFIHADGGARSSSCRKQIDVLLNSMNEKELV
                     VIEATAEGIKKARETEVPE"
     gene            complement(23984..25354)
                     /locus_tag="ACOIAF_001307"
     CDS             complement(23984..25354)
                     /locus_tag="ACOIAF_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005927602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HGZ2668323.1"
                     /translation="MNKSERFFRRYIFSIVSIIILFLFVNILLVASFFAAAYLGNVKN
                     SNFPIEDFSNHITETNGQFNADIQAEDMLNNACAWAMILDENGNIIWEMNVPEELPRH
                     YSTTDVAMFSRWYLNSYPVNIWNRQDSLLVVGFPQGYVTNYYTSIISQYVRPIAFGFL
                     AAVCINVLLMLYLFVRNAHRIEKAMEPILNGIRHLSSGHPVHLEENGELAEINAGLNK
                     AGDYLLKKDNTRAEWIRGISHDIRTPLSMILGYASEIEETSSLPETTRKQAEIIRKHS
                     EKLKNLVENLNLTTKLEYSMQPMQKQTLVPVELARQAVSEVLNDGLSDKYELELSEDN
                     PGKAITIIGDQSLLNRMLCNLIRNCIVHNPNGCRIQVSVGSCDRTCTFSVIDNGCGIS
                     EPQLNTLNNDKDISSTQKQTGEIEHGLGLKIVRQIVKIHQGTIQFSDTAPHGLSVEVV
                     IPIEIY"
     gene            complement(25351..26046)
                     /locus_tag="ACOIAF_001308"
     CDS             complement(25351..26046)
                     /locus_tag="ACOIAF_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGZ2668324.1"
                     /translation="MNDKRILVVDDETDLLEMVKSIFTRAGFTQVLTASSGEAALKMC
                     EEQEPDMMILDVMMPGMDGFAALKELRKTSKIPVLMLTARGEAEDKFVGFENGADDYL
                     LKPFLPKELLFRVQAILKRVYPEKERKVFFDAATVDLEKAIVQRSGENISLTAKEFQL
                     FEKLYENQGRIVTTGALCQAICGDFWQGYESTLATHIRHLREKIEENPSKPVALVTIK
                     GIGYRLNIKGVQT"
     gene            26306..27481
                     /locus_tag="ACOIAF_001309"
     CDS             26306..27481
                     /locus_tag="ACOIAF_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HGZ2668325.1"
                     /translation="MIYVGIDVAKDKHDCFITNSDGEVLFKAFTIKNNLDGFDELYQK
                     IESVMEDASKVKVGLEATGHYSYNLLGYLLDKGLATFVINPLHTNLYRKSLSLRQTKT
                     DKVDAHTIASMLMSDVNLKSYSDTSYHNEELKSLTRYRFDKVKERAKLKTSISRLVCI
                     LFPELEKLVPTLHQNSVYELLYEFPGAKQVANAHLTRLSNLLETASKGHYTKETSIAF
                     REAARTSIGSNMPAKSLELKHTIKLIRELDSEIEEIENEIKVIMDEINSPILSIPGIS
                     YRMGAMIIAEIGDFSQFDSPDKILAYAGMSPSTYQSGQLDNCYARMEKRGSRYLRYAL
                     FNATAYVCLWDPTYKAYLAKKRAEGKHYYVAMSHATKKLVRLIYHLERTGQQYQKAI"
     gene            27787..28710
                     /locus_tag="ACOIAF_001310"
     CDS             27787..28710
                     /locus_tag="ACOIAF_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2668326.1"
                     /translation="MNDYIIETHNLTKKYGSQASVSHLNIHVKKGRIYGLLGRNGAGK
                     TTTMRMLLGLTAPTSGEVTIFGKPFQGNEKNILPRIGCLIESPGFYPNLTGTENLKIF
                     AELRGLRSPQYIKNALELVNLPYRDKKLFSQYSLGMKQRLAIALAVMHNPELLILDEP
                     INGLDPIGIAEVRDFIRELCDTSGKTILISSHILSEISLLADDVGIIDHGKLLEEESL
                     KELEAKNAKFIHFCVSDAQKAAKIIAATFHSKDFRLTDANNLYLYDMTLPVERINRSF
                     VESGIEIQEVHLCEDTLEDYFKKITGGEGIA"
     gene            28694..29506
                     /locus_tag="ACOIAF_001311"
     CDS             28694..29506
                     /locus_tag="ACOIAF_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006874368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2668327.1"
                     /translation="MRGLLNLIRCEFWKLKRKMFVQLVLAASFLFPIPLTAIIYYLNT
                     AQNKYATKAAAFDALWQSVIGFGMLLLLPCILGIVAALLFFMERDNDTFKNLRTIPIT
                     SSQMVIAKCIVLLLLSIAFCISSTVASILCGFVFFGVTDILFKVLFSAVYGLLIAISA
                     LPLILLIVFFSRSYIFSIMLCVFYSVFNLLSTFSMTALPKWLVLTLSTPSIMLWGTQQ
                     MSSRTAINDTEDLQNFIQMGLIPSTPQLIITLGVIGAVSLLLAVYLYKKRSE"
     gene            29508..30278
                     /locus_tag="ACOIAF_001312"
     CDS             29508..30278
                     /locus_tag="ACOIAF_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009255077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2668328.1"
                     /translation="MVTIIKTEFMKLKRYFIVWIGVSLMLLTVLLTLFTTMADDRMVW
                     DYQFLFEQVVKHFVTMIFPMCITLISGYMISREYTDDTLKNIETLPISFRKLLAGKLI
                     VSAILSFFLGIVCFAFTVVANFIMGYDGFALIPALTGLVQMALLGFFLYLTMLPIIVL
                     TSRYKGSFLVGVIVAFVYGFIGMFANGTLQSIYPVSAALGLINYRAGAEGVMWNKGLC
                     FISILIMCAIGIALMFVKQKPEKREAKKTQHTAPKKGW"
     gene            30299..30646
                     /locus_tag="ACOIAF_001313"
     CDS             30299..30646
                     /locus_tag="ACOIAF_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005927589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HGZ2668329.1"
                     /translation="MSSKKKIGIVIVVAVLAITFMTVAFLTTQTNTDYYTQIDNRRVT
                     DIAPHGAMNYKYTLVAYDESGNAKDVTFETSKVLTDGAYLCLKVAPIRGVVTWAEMQF
                     DELPSSVQEKYKE"
     gene            31143..31565
                     /locus_tag="ACOIAF_001314"
     CDS             31143..31565
                     /locus_tag="ACOIAF_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HGZ2668330.1"
                     /translation="MAIINLRDYYPFYTSDCFMEVSEEVAEMFKEFDRKEAAYRLRTY
                     RHKAYYSLDRDDGLEHEAVFVALSPHELYERKVTMQELHAAIASLPDKQAKRIYAHFI
                     LGMTKQDIARAEGVHEKVVRVAIERGLRRLEKILKNSL"
     gene            32007..32276
                     /locus_tag="ACOIAF_001315"
     CDS             32007..32276
                     /locus_tag="ACOIAF_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015564338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668331.1"
                     /translation="MLSDDRPTDPAWLPIFSKKEHFMGNAFGMIPGLEPDEWSNDACR
                     GYVIMAMEDCGFSKKDIRRVVGQLYEVFDLNSVEDAKEKYHSSPY"
     gene            32410..32586
                     /locus_tag="ACOIAF_001316"
     CDS             32410..32586
                     /locus_tag="ACOIAF_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005927581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668332.1"
                     /translation="MTQAVTYERETKSVAFQGKIIVLESLTPVLPPKEKEQRKKEIER
                     CLYEVFSKYGDRFP"
     gene            32676..34184
                     /locus_tag="ACOIAF_001317"
     CDS             32676..34184
                     /locus_tag="ACOIAF_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015567049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HGZ2668333.1"
                     /translation="MEFIREDCIYARQSVDRKDSISIESQIDFCKYELKGGSCRIFKD
                     KGYSGKNTDRPEFQKLLGEIRKGKVRRVIVYKLDRISRSILDFATMMELFQEYDVEFV
                     SSTEKFDTSTPMGRAMLNICIVFAQLERETIQKRVTDAYYSRCLKGFHMSGQAPYGYQ
                     LEPTVVEGIRTKKMVADPVAADHVRLMFEMYAEPETSFGDITRYFEEHDIKIYGKSMF
                     RTFLSQLLRNPVYAQADLELYEFFKSQGAAIVNDASDFAGTNGCYLYQGRDVKEDKDR
                     CLKDQILVIAPHEALISSDTWLKCRKKLMANTTFQQGRKPKNTWLAGKIKCGHCGYAL
                     KATHVPNSTGYFRCTKRTENKGCPGCGKIRKEEFEQFIFSAMQEKFKDFQILHGREEK
                     VNPKLTAYQVELAQVEAEIEKLLDTLTGANATLLAYANKKIEELDTRRQTISKAIAEL
                     SVETISPQQIKKLSYYLDNWDSIDFDDKRKAADGLISTIKATSDHVQIEWKI"
     gene            34291..34569
                     /locus_tag="ACOIAF_001318"
     CDS             34291..34569
                     /locus_tag="ACOIAF_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3324 family protein"
                     /protein_id="HGZ2668334.1"
                     /translation="MSYKKAIHILPEELLELIQEYVDGECIYIPRKSNNKKEWGSNTP
                     TREELSIRNMQIYEDYQVGYDLQYLSEKYYLSLKSIQRIVLQEKRKDI"
     gene            34862..35437
                     /locus_tag="ACOIAF_001319"
     CDS             34862..35437
                     /locus_tag="ACOIAF_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1062 domain-containing protein"
                     /protein_id="HGZ2668335.1"
                     /translation="MKKITWEVEYISQLPVIRYCKKCGKKTEYICSGLFRVNAQHKYL
                     DIWLIYKCSNCDSTWNSTIYSRVNPKSLNPEILEQFHTNDYNLVKQYAMNMELLHRNG
                     AEVGIPEYKIVGQEININDLTQVHIISKYPCQLKVSTLLREKLGLSRKVFDEMLDCGI
                     IRSNSGVDLKKGKLNYEIMLDIGKEKQKSSN"
     gene            complement(35543..36220)
                     /locus_tag="ACOIAF_001320"
     CDS             complement(35543..36220)
                     /locus_tag="ACOIAF_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HGZ2668336.1"
                     /translation="MNPDDTLLKECADLIFSTWREDGRFKLYPNGGIYPCHTANAANV
                     LCHMGYASDIRMQKTFQHFLDTQYTDGGWRCNKFSFGRGPETEYSNPLPTLNILNAFR
                     FSNYLNKESKLDKAVDFLLDHWTIKKPIGPCHYGIGTLFMQVEYPFRNYNLFLYVYVL
                     SFYNRAKKDNRFLEALKILESKMIGNKIVVERVVPKLSKLSFCKKGESSEIATTHYYE
                     ILKNLEK"
     gene            complement(36444..37073)
                     /locus_tag="ACOIAF_001321"
     CDS             complement(36444..37073)
                     /locus_tag="ACOIAF_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3786 domain-containing protein"
                     /protein_id="HGZ2668337.1"
                     /translation="MDEKITKNNYNVPFDYVRKKLKELDPHTISNLSNIPFNNDLNRF
                     TLNFFDRQYIIDYPSGEVWNSNGSLCNNFELKILIVRYLINAKGIPRTNKYVTFKEIP
                     GGNVYYPNFLNRTLYRLSEVFTSQIDKYKLVMEKIGAEKVNMGDLAYRFTYMGNTSMV
                     FILWFGDDEFPPNSNILFDSNIVYYFNAEDLAVVPDTAINAILNKINQL"
     gene            37384..38043
                     /gene="cprR"
                     /locus_tag="ACOIAF_001322"
     CDS             37384..38043
                     /gene="cprR"
                     /locus_tag="ACOIAF_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CprR"
                     /protein_id="HGZ2668338.1"
                     /translation="MSKILVVDDELDMLKLIENVLKRDGHEVKVISDVEKAMNMDFNY
                     FNLIILDVMMPKMDGFEVCKTIRNKVDCPILFLTAKNMESDIMYGLGIGADDYITKPF
                     GVGELRARITAHLRRESREKKNLLTISNVQFNFLGKEVSIDGKKINFTKSEYLICEFL
                     AKNKGQVFSKERIYESVYGFDGESDISTITEHIKNIRSKLKQYDIYTIETVWGIGYKW
                     V"
     gene            38254..>38917
                     /locus_tag="ACOIAF_001323"
     CDS             38254..>38917
                     /locus_tag="ACOIAF_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HGZ2668339.1"
                     /translation="MDRLKNIRKSLLQYTFLFLLIGITIYLVFKTLDIKMLSNVMVMV
                     DKKFLFIGALAIMCHIMLEGVVMKIIIESTHKVNIRFIGFKLAIMGFYYNLITPFASG
                     SQPVQIYVLKKCKMPLSKASAVVTNKSIIFQIVVTFYCTILVLMNFAMLEKQMKMIMP
                     LVFLGIAINSFVLIMIILVILNPKKIKYFMRITIKHLSKIKFLKFLDNKIESIESFID
                     DYS"
BASE COUNT        11007 a         8731 c         9604 g         9575 t
ORIGIN      
        1 tggagatata aattattccc cgtcgccagt tattccgtag ctagccggga aaatcagtca
       61 agggcagcgc cgccgcaggc ggtgccggag gcacccttga cggatttctc ccggttatgc
      121 tttttcccgg tcaagcgacg gggatataaa ttctccagag ccgttcccct tcccggggga
      181 aggggcggag gggttgggcg ataccttgct ttttcctaaa ccaaaacaga aatggaggct
      241 caaccaatga aacgaccttt agcgtacctg accgccgcat ggagcggcga gccggatgtt
      301 gatatggagc tggcggccca ctactgccgc cttgcttatg aggcgggctt ttcccctatc
      361 tgccccctct tgtacctgcc gctgtttttg aatgacagcg ttcccgagga acataaggcc
      421 gggattgata tgcgccggga tatgctgcgg cgttcccata ccctgatcgt ctgcggcagc
      481 gttgtggacg aggatgtgaa aaacgatatt gcggttgccg ggcggctggg cattgcggcg
      541 accacactgg aaggggtgct tgccgtgaag ggacatggta tcccgggcca tgccggacat
      601 taagctggga agcctttttg acgggatcgg cgtgttccct ctggcggctt cccgttgcgg
      661 tatccgtccg gtatgggcca gcgagattga aaaagcgccc atctccataa ccaaaaggca
      721 ctttcccgac atggtgcatt tgggggatat tacgaaggtg gatggcggga aaatcccgcc
      781 ggttcatgta attaccttcg gttccccctg tcagaacctt tctctgatcg gcaaccgctc
      841 cggccttgcc ggggcaaaat caagcctgtt ctatcaggcg tttcgtatca tacaggaaat
      901 gagggatgct actgataacc tatatccagc tatcgctgtt tgggaaaacg tcatgggagc
      961 gttttctaca aatgaccgga tggactttag agccgtccta tccgccttct cggacaccga
     1021 agttccaatg cctccttcgg gaagatgggg aaacgccgga atggtgcgag ggggaacgcc
     1081 tgatgtgtgc tggcggctca tggacgccca gtattgggca ggcacccgaa ggctggcacg
     1141 aaggcagcgg attttcgtcg tggcggattt tggaggcaga cgtgccgcag acatactatt
     1201 taagccccgt ccaatgctcc cacttcctcc gccttgcgga gagggcgggc gggccgccgc
     1261 cgaaggagat cgaacagctt cttttgaaac agggcggcag ataccagtca tccacccctt
     1321 tcagtgcttc cgtatgcggg gagcggcaaa aaggcaggaa gaaacggcct tccaaaacag
     1381 cttcggatta ccaactgacc cttttcccac tcttttagcc agtgacgtga cgcccttcgc
     1441 cttctggtat gagggcgacc cggagggcgg ctgtatccgt tttctgacgg aaacggaaag
     1501 cgaacggctg atggggctgc cggagggctg gacaaagtac ggggcggacg gcgtggagat
     1561 ccggcctctg caacgctaca aggcgctggg aaatgcgatt gccctccctt gcgccgatta
     1621 cattatggct gggatttatg aggtgctggc tgaccgggcc ggaaaggagg aatgagccca
     1681 tgtttgaagc ctatataacc aatacagccc tatacccctt aatggggatt gaggtaggga
     1741 caacggtaca tttccccatg acgacacagg agttgcaggc cgcccttgcc aaaatcggga
     1801 tagacgggaa acggtacagc gaagtgttct ttatcagctt tgacagcgat gtgctggggc
     1861 tctacgatca tctctacgaa tgtgaggaca tcgacgagct gaacgagctg ggccacgccc
     1921 tgctggaagt acgggataag ggcggactgg aaacctttga agccgctctt gtcttgggaa
     1981 accacacaag gagcgtgaag gatttgataa acctgacgca gaaccttgac ctgtaccgct
     2041 tttacccgga tatttccgat gatgaagggc tgggccgtct gtacgccgac gagctgggga
     2101 ctatcgacat accggagcac attcagaact acttcgatta tgaagcatac gggcgggatg
     2161 tgcgtatcaa cgaaggcggc gtattcgctc ccggtgggta tgtgtcggca gtcccggagg
     2221 gcttcaagga gtattatcac ggaccgcagg acatcccgcc ggaacaccgg atatttgcct
     2281 atcccgaaaa ggccgagcct gtccactcca ttctcgccgc actcaaacgg tttcaagagg
     2341 ccccgcccgc tccgaaaaag gacaaggcgg ggccttccca tgaagaacgg taagacttcg
     2401 ggccagcatg gcccgaagca tgaaggaggt gcataccatc aactattacc ctatcaatga
     2461 agggaccgcc cgccgggcaa aagaaatgaa cagcttttcc gactacaagg aagggagcgc
     2521 aacggcggaa taccgggcaa tggtggacaa ggccgccgcc atagcggaaa agcagaaatc
     2581 ccgggtggac cccatgtacc atgagaagat cgaccatctg ttagacacct acgcccgcaa
     2641 gctggccgaa aacatgaacc agggctttgc cattgacgca agggttccct ctatgatgat
     2701 cgccggacct gccaatttcc cggtgggaaa gaaagaaaaa cagaaccggg cccgggacag
     2761 caacatggag gaatggcggt atattcaagg gctgctggat aagatacgca gtaccggcat
     2821 gggcgggatc agcgccgatg acccggcagc gattgaaaag ctccagaaga agctggacgg
     2881 gctggaacgc tcccagctca ttatgaagga ggtcaacgcc tattaccgca agcatggcaa
     2941 gctggacggc tgtgcgctgc tgtcgccgga ccagattgaa aagctgaaag caagcatggc
     3001 gtcaagctgg cggagcgacc cgaggccctt tgaaagctac cagcttacca acaacaatgc
     3061 ggagatccgc cgggtaaagg cccgtatcga acagctttcc aaacaggcac agcgggagtt
     3121 ttccggctgg gagttcgatg ggggccgtgt ggaaatgaac cgggaggaca accgcttgca
     3181 ggtattcttt gatggaaagc ctgacgcgga cacccgggcc gagctgaaaa gcagcggctt
     3241 tcgctgggct cccagcgtgg gcgcatggca gaggcagctc acggacaacg ccatccgggc
     3301 ggctgaccgg ctggaatgta tcaagccgct gtccggcgaa aagccctccc agcttcaaaa
     3361 gaagccctct atcttgcaga ccatgcggga acagggcgaa aaagtccaga cggagccgga
     3421 aaagaaagct ccgtccggca gggatgccga gcggtaagcc tcgggccaca ttggcccgag
     3481 gttttctgtt ccccgagatt gtacgggggc ctcccggata gcgggaaccc cgacggaaag
     3541 gaggttttat gcaggaagaa gtaaaccaaa aaacggttgc cctttcgatc aggacaacga
     3601 agctcacggg aaaagtgctg gctgccgctc ttgggaaggt agcgcgggcg ctgcaaaagc
     3661 accaccggaa ggcgctgacc ccgcagggac gccagagcgt gaaaaagctg atgaaccact
     3721 atggcaccaa aagcgccatg ccctatgtgg gagccccaaa ggattttgac cggattgcga
     3781 aggagttcca tgtggactac gctttccata aagtgagccc cggtcattac ctgctgtttt
     3841 tcaaagccaa tcaggcggac gctatcacgg cggccttcca gaagtacagc gcaaaggtgc
     3901 tgaacaaaga gcaggacaag gcttccatcc tcggccagct tcggaaattc acggagcaga
     3961 tcaggacaca ggcaaaggaa aagcagcgga ccagagaggc ggtgaaggac ggacgttgag
     4021 tgacaagatc agaaaatatg tgctcccaaa cctgccgtac ctctttgtgt tctggttctt
     4081 ctccaaaatc gggacggcct accggatcgc ccccggcaca gacttcggga caaagctcat
     4141 ggggatgctc gacaccttcc caaaagcctt tgaaacctac tggccggggc tgggaggtat
     4201 tgacctgctg gtgggccttg ccggtgcggc tggggtgtat ctgctgatac agtcaaagat
     4261 caggcaggcg aaaaaattcc ggcgggatgc ggagtacggc accgcccgct ttggaacaaa
     4321 ggaggatata aagccgtttg ttgaccctaa atttcagaac aatgtcattc tgaccgggac
     4381 ggagttcctt accatgaaca cccgtccgaa gatacccgcc aatgcccgga acctaaacgc
     4441 ctgtgtcatc ggctcgtccg gctcgggaaa gacaaggttc tggctgaccc cgcagctcct
     4501 tcaagcccat tcctcgtatg tggtggtaga cccgaagggc ggaaccctcg accagtgcgg
     4561 gcggtttctg caacgggaga aatacagggt gcgggtgttc aacagtatcg acttttcaaa
     4621 atccatgcac tacaatcgtg cgacttgttc gtcgctgaaa aggcggcgca cagcgctgtt
     4681 tttaatcagt tctgtgaact gataattcga gaggtggaat gatagggtaa cgccttgaaa
     4741 cgcctcccct gatactccgg catgcgtcat cactctgtga tgaggtgtga agcccggtaa
     4801 agtcggcaga aaggtgccgt aacagataac gctgacgaaa cctgtccaga ggtggacagc
     4861 gcaccccata tgccgggggt ctataaaata cctaaggtga gaatggcagc aatgcctgac
     4921 gaaactcccg aatgtaaagg tctaaaagtg ccacatacag aaatgtatgt gtgggttcat
     4981 gcccagacag tgcggtctag taggaatggt tgttacgaac caccgtgatg tgttacaggc
     5041 acatgcaagc tgtcaggctt agagggaaca cctaagggta tatatgcaca gatagaatta
     5101 tcggaacaag gaaaggcaga gagtcctatg gataatccgg cgaagaacaa taagcggatg
     5161 aatctctgct gaaaggtcgt ggtcgaactg tcgatgttgc ctgcgaaaaa gagggcgacg
     5221 gaggaatggc cacaagtcag gcagaaaatc agcagtacac actctctaat catgcagtcg
     5281 agtaagaata agaaattgtc actcgaatca atgaaatggg gtgatgtaca tgacacaaaa
     5341 agctaaaaaa agaagcaaac tgcgccatgc ggaatactac gacatgcaga acatctttga
     5401 tgaactgtat gcaaaaagca aagatggaga aatattcaat aatctcgtgg aaatcattgc
     5461 ggcgccaaat aacatcatgc ttgctttcag aaatattaaa agcaatgatg ggagccatac
     5521 cgccggcaca gatgggagaa cgatagaatc cctggcagat atgtcagaag aagatttcgt
     5581 ctctcttatt caaaaacagt ttacaaggta tgtgccaaaa acagtaagga gggtagagat
     5641 tcccaaacca aacgggaaat ttagaccgtt gggtataccg tgtatcatag acaggattgt
     5701 acagcaatgt atattgcagg tcatggagcc aatctgcgaa gcaaagtttt atgaacattc
     5761 atacggcttc agaccttgca ggagtgctga aaacgctgtc tcctacgctt acggactggc
     5821 tcaaaggaat aagctccact atgtggtaga cgtggatata aagggtttct ttgacaacgt
     5881 aaatcataga aagctgttac aacagatatg gacgctgggt atacgggata ccaaactgat
     5941 tcagataatc aaagcaatgc tgaaagcgcc aattcaaatg ccagatggag aaatcttata
     6001 tccagacagg ggaacccctc agggcggcat actttccccg ctgttagcca acattgtcct
     6061 gaacgaactg gactggtgga tagcgtcaca gtgggagggt atggcagacc atatgaagtc
     6121 tccttgtaag gtcacatatt atccgaatgg ggcagagaaa aagtgcaaca gctacacggc
     6181 gctcaaaaag agcaatctca aggaaatgcg tatagtccgg tatgcagatg attttaaaat
     6241 cttttgcagg aaccgtaagg acgctgacag agcataccat gcggttaagg actggctgct
     6301 aaagcggctc aaactggaaa tttctgatga aaagtcaaag gtcacaaatc tcaggaaaag
     6361 agacagtgaa tttctggggt tccgtatgaa gctgataagg aaacgcagga catgggtaat
     6421 cagttcaaat gtctgtgaca aggcaatgtc gcatatgcag aaagaactct caaaagcaat
     6481 tatagagatt caaaacagca agacagcaag taatataaag agcaacattc acgaatacaa
     6541 cgccaaagtc ataggtatgc agaactatta ttgcattgct acgaggataa atctgaattt
     6601 cagccgaatg gctcatgtga ataacggtcg ttttctccat cggatagatg gatataaaaa
     6661 gcagggcgag ctaaacaaca aatatttaaa aaaccggtat ggaaaatcga agcaaatgcg
     6721 ctggattgga gatacaccag tagtacctct ggcatatgtg cagtttaaaa taccaatgcg
     6781 caagagcagg aaaatcaatc catatactcc ggacggaaga atggaaatcc acagcaatct
     6841 gaaactgaat gttgaagata tgctctggct catgcgccac ccggtaatag gagaaacggt
     6901 acagttcaat gataaccgaa tctctctcta tgccgggcag gaaggtaaat gtgcaatcac
     6961 tggggaacaa ctggaagtga ttcactgtat ttgttacagg aaatcaaatg agtgtaaaaa
     7021 tggcagagac agttaccgta atctgttgct tttatcgcct gccggatatg aatttatctc
     7081 aacaacagac agtataaagt tcgctacact gacaaagaag tatgaattga ataaggcaca
     7141 aattactaag gtaaacaaac tgcgtgttac cgcagggttg gcagaaatgt caatgtaaat
     7201 cgtaacaaac tgatttagct aatgtgaaaa agtgagtgtg tatcactgct gaaactatgc
     7261 ttgatggggc gccggatgcg gtgaaagtcg catgtccggt gctagccggg ggaaaagctg
     7321 gagataacat caaaggctta cctatcggaa ccgctggcct atatcaagac ggaaagcgat
     7381 gttttgaaat tcgttactgc tctgatcgcc aacaccaaag gcgacggcaa ggagggcgac
     7441 gagttctgga ctaaagctga aaccctcttg tactgcgccc ttgtggccta catcgtcttt
     7501 gaggggccgg aggaagaacg caacatgaat acgctggtgg aaatgataaa cagcatggaa
     7561 gtccgggagg atgacgaaac cttcaaaaat gcggtggact atatgtttga cgggttggaa
     7621 cgccgcagcc cccagcactt cgccgtgagg cagtataaga aatacaagct cgccagcggc
     7681 aagacagcca aaagcatctt gatttcctgc ggtgcaagac tggctccctt tgatattccc
     7741 cagcttcggg agatcatgtc ttatgacgaa ctggagctgg ataagctggg ggatgaaaaa
     7801 tcagcgctgt tctttcttat cagcgacacg gacaccacct acaacttttt ggttgccctc
     7861 gctttttcgc agatgttcaa ccttctgtgt gaacgggctg ataacaccta tggcgggcgt
     7921 ctgccctacc atgtgcgggt gctgtgggac gaggctgcca acaccggaca ggtgccgggg
     7981 ctggaaaaga tcgtggccgt catccgctca cgggagatca gcctgacgct cttttatcag
     8041 gctatgagcc agtgcaaggc attgtacaaa gaccattccg aaaccatcat gggcaacatg
     8101 gacagtatcg tgttcctcgg aggccgggag gcttccaccc taaaggatat ttcggaaaac
     8161 tggctgggca aggccaccat ctctatgcag accgagggcc gaagccgtgg acagtctgaa
     8221 agttacagcc agaatatgca gcggcttggc cgggaactga tgaccaccag cgagatcacc
     8281 accatgcccg gagataaatg tatcttgcag cttcgggggc tcccgccctt cctgtcccca
     8341 aagtatgact tgaaaaagca cccgaattac aagtacacag ccgaatttga taaaaagaaa
     8401 aacgccttcc gcttggaaag cctgttccgc caccggccat tgagactaaa gccggaggac
     8461 gaatacacgg tgtacgaagt ggacggctcc gacacggacg aagaagctga cctgttgaac
     8521 ttcgatgatc tggacagcga cgagtttgtg taacttcggg ccatgatgac ccgaagcgcc
     8581 gccgatgtgg gcggcttttt ttgtacccaa aaccatcaaa caaacaaaat ggaggaacat
     8641 atatggaatt tttcaatcag gcaatcgaca ttctcaaaat tctggtcatg gctcttggcg
     8701 ccggtctggc ggtatggggc gtcatcaacc ttctggaagg ttacgggtcg gacaaccctg
     8761 cggcaaaatc acagggcatt aagcagctca tggcaggcgg cggtgtcgtc ctgatcggcc
     8821 ttcagcttat ccctctgctg tccggtctgt tcagctaagt gtcacccgtt gtcccttttt
     8881 ccagaaaagg agctgatgta tggactttat catcgacgca atcgttgaat ggctgaaagg
     8941 tctgctcgtc gatggtatca tgggaaatct ggacggcctt ttcgataacg tcaatcagag
     9001 cgttggcgag atcgccacac aggtaggcac gaccccggcg gactggaacg ccggggtctt
     9061 ttccatgata cgacagatct ctgaaactgc cattctgcca atcgccgggg tcatcctcac
     9121 ttttgtaatg acttatgagc tgatacagat gcttatagaa cgcaacaacc tccatgaagt
     9181 tgacacatgg atgtttttta agtgggtgtt caaaaccttt gtggctgtga tgatcctgac
     9241 gaataccttc aatattgtgc tggcggtctt tgatgtgagc cagcatgtga tacagcagtc
     9301 agcgggcatt atccagaacg ggacagagat cacgccggat gtgctggaca gtctgcggac
     9361 agaacttgag gtgatggagt taggccctct gttcgggctc tggctacaat ccttcctgat
     9421 ccagcttacc atgattgccc ttaatatcgt gatcttcgtc atcgtatatg gccgtatgat
     9481 tgaaatttat ttactcacaa gtttagcgcc tatcccgttt gccaccgtcc ccaacaggga
     9541 aaccggacat atggggcaga actatttccg ctccctcttt gcggtgggct ttcaaggtct
     9601 gttgatctta gtctgtgtcg ccatctatgc ggtgctgatc cagagtatcg ccaccggcgg
     9661 cgaccccatc ggggcgatct ggggctgcgt gggatatacg gtgctgctgt gttttacctt
     9721 attcaaaaca ggcagccttg caaaatccat cttcggctgc cattaagaga cttcgggcca
     9781 tgctgacccg aagcggaaag gaggatcatg ccgaaagaaa aattttcgat aatctatgca
     9841 gatccaccgt ggcggtatga gcagaaaaac aggcagggat cagcagagct tcattatccc
     9901 actatgagta ttgaagaaat ctgttccctt ccagtggccg acttggctgc aaaagacagc
     9961 gttttgtttc tgtgggcaac attcccacaa ctgccggaag ccttaaaggt cattaaagca
    10021 tggggctttt cttatcgttc tgtggctttt gtatggataa agaggaaccg gcgctcctac
    10081 tcatggtttt acggtctggg gtattggacc agaggcaatg cagaaatttg tcttttagca
    10141 gtaaaaggac atccgaagcg taagtctgcc ggtgttcatc agcttattgt ttccccgata
    10201 gagcgccaca gtaaaaagcc ggacgaggcc agacggaaaa ttgtagaact gatgggcgat
    10261 ataccacgag tggaattgtt cgccagagaa aaaaagcccg gatgggacgt gtggggaaac
    10321 gaagttgaaa gcactgtaaa cttgtaacag cttcgggcca tgctgacccg aagcggaaag
    10381 gagaaatcca tgcaggaaaa aactgtgggc ggaacaccag ccgcccctat aaagctggat
    10441 gtaagcgtgc gggtcatcga acctgtgaaa aacctcatgg gctttgccag cgtgaaattc
    10501 aatgactgct ttgtggtgga aaatctgaaa attgtacagg gcagcaaggg tctctttctt
    10561 gggatgccca gccagccgga cggcaaaggc ggctatcggg atatggccta ccctgtcaca
    10621 aaggagttcc gggaacagct caacaccgct gttctgcaag cctatgaggc aaagctggaa
    10681 cagatggcgg agcgcggctc tgtggggcgt gcgtccatca gcgaccagct caaagccgga
    10741 aaagcggctg ccgaacaggc aaaggcggaa cggcctccga aggaaaagcc cagccgcagc
    10801 gcagagcgtt gacctcgggc catgctggcc cgaagcagaa aggaggcggg aaccatgcca
    10861 cgcaaacgga tgggctatga cgcggcctgc tattacgacg gaaaactgct gggccgctgt
    10921 accaaagcgg acagcgacgc ctataccctg ttgatgaacg cctgtggtgg ggacgccgcc
    10981 cgggtcctgc gggaatatgc ctatttttcc ccggaacttc gcgccatatt ggaaaaggcg
    11041 gcgctcatgc aggcggaccg gaaccggacg ggcggaatgt tccatgcgcc gaaaagttcc
    11101 ccgtggggcg aggtgcagag ctgtgaaacc ctctgtccgg gggtgtttct ggtatctacc
    11161 gccagccacg gcggaacgat ggtggcaaat gaggtagccg ccgtcctctc cccagcggcc
    11221 aaaaaatgcg gcttcaagga taagggctat atctgctatg aggaggacgc acaggaaagc
    11281 gtagtgctcc gggaactgct ggacaaaaag ctgtggaaca tccctgaccg cattaaggac
    11341 aaagggcagt ttgaagaaaa cctcaatcag tccatccggc agtatcaccc cgaatactgg
    11401 cgggcaaggc agagcggacg agaagccgcc gaagccgccc gcagtacagc cccggccaaa
    11461 gaggccgcaa gataacctcg ggccatgctg gcccgaagca gtgaaggagg tgtacccaaa
    11521 tggcctatgt gccagtacca aaagaccttt ccaaaatcaa gacaaagctg gccttcaatc
    11581 tgacgaagcg ccagcttgtt tgtttttccg gcgcggcggc ggtggggctc ccggcctacc
    11641 tgctttcccg tggcagtatc gggaacagcg ccgccatgtt cctgatgatc ggcctcatgc
    11701 tcccgttctt ctttctggct atgtatgagc gggacggcct gccgttggaa aaagtgctga
    11761 aaaacatcat ccgcacccgg ttcctctatc cccgtgtgcg gccttacaaa accgaaaact
    11821 tctatgcgct gcttagcgcc agaaaggagg cgcagccgat tgcgaaacag cagaagggcc
    11881 ggaaagcccg caggcagaaa gcctgaaaag cagggcctta ccggtctgtt cacaaaggga
    11941 aagaacattc ccaccaccgc ccagcagacc ctcccttacc gggaaatgta ccgggacggg
    12001 gtgtgccggg tagcggacca ctattacacc aaaaccattg aatacgagga catcaactac
    12061 cagctcgcac agtccgaaga tcaggcggcc atctttgacg ggtggagcgc ctgcctcaac
    12121 tactttgaca gcagccttcc gttccagctt tccttcctca accaccggag ccgcccaggc
    12181 agccggtaca gtgtgaacat ccccatgcag gatgacgatt acaacagcgt ccggtgtgag
    12241 tatgtggaaa tgctggaaaa ccagatcgcc aaaagcaaca acggcattgt ccgcacaaag
    12301 ctcctgacct tcggcgtgaa cgtggacgac cttcccaccg ccagggcaag gctggaacgt
    12361 gtagaggcgg acatttgcgg gaacttcaaa aagctgggcg tcaagtgccg ctccctttcg
    12421 gggctggaac ggctggagct tcttcacggg cagctccacc ccgacagcgg ctcccccttc
    12481 cggttttcat gggatatgat ccccaaaacc gggctttcca ccaaagactt tatcgccccg
    12541 gacagcttcg acttccgttt cagccgactg ttccgggtgg ggacgacttg gggcgctgcc
    12601 tcctacttgc agattttggc ctcggagctc tcggacaagc tgctggcgga gcttttggaa
    12661 atggatgcgg aaatgaccat caccctccat atccaaaccg tcgatcaggc cgccgccgta
    12721 aaatccatca aggccaaagt ctccgacatt gacaagatga aggtggagga acaaaagaag
    12781 gctgcccggt cagggtacga catggacata cttccgcccg acctcgtaac atacagcaac
    12841 gacgcaaaga ccctcttgga agatttacag agccggaatg aaagaatgtt cctgctgacc
    12901 ttccttgtgg taaacatggc cccgacccgc cgggagctgg acaacgacct gttcacggtg
    12961 tccggtatcg tccagaaata caactgcacc ttgaagcggc tggacttcca gcaggaggac
    13021 ggttttcttt ccagccttcc gctgggccat aacggcattg agatcaagcg cggcatgacg
    13081 accagctcca cggccatttt cgttcccttt atgacgcagg agctccgcat ggatggcgaa
    13141 gccgtctatt acgggctcaa cgcactttcc cataacgtca tcatggcaaa ccggaaaaag
    13201 ctcaaaaacc ccaacggcct gttcctcggc gtgccgggct ctggtaaatc cttcgccgca
    13261 aagcgggagc ttgtgaatgt gttccttgcc acaaaagacc gcattatcgt ggtggacccg
    13321 atgggcgaat actcgcccct tatcaggcgg ctgggcggac aggtcatcga gatcgccccg
    13381 gacagccccc accacatcaa cccgatggac attgatttga gcttcgacga ggaaaacccg
    13441 atggcgctga aagccgactt tatcctgtcg ctgatggagc tgatcgtcgg cggcaaggac
    13501 ggcttgcagc cggtggagcg taccgtcatt gaccgatgtg tgcgccagat gtaccgggaa
    13561 cacttgcagg acccggaaac aagcaaaatg ccgaccctcc aaaccctgta tgacctgctc
    13621 tgttcccagc cggagggcga ggcggtgcgg ctggcgacgg ctcttgaaat ttatgtgtcg
    13681 ggttccctta acgtgttcaa ccatgaaacc aatgtggact tgaaccgccg tctggtatgc
    13741 cttgacttaa aaaagctggg ggccgggctt cggacgattg ccatgctcat tatgcaggac
    13801 ttggtaaact cgcaggtgtc catgaatttc ctccgcggta tcgctacatg gtgctacttc
    13861 gacgaatttc atgtgctgct ccgtgaccgt ctgacggcaa gctattgtgt ggcgatctgg
    13921 aaaatgctgc ggaaaaaagg gtgtgttccc agtgctttaa cgcagaacgt gaaggtgcgc
    13981 ccagataggg cgctgtgcaa agcagtttaa ggtactgccg cacacggcag tgtgcgaaac
    14041 aatctgcctt accgactaag gagaaatcct ttacgggaac agcgcatggc agaaaaagga
    14101 atagtcacca aaggacttag gtgcgactgt ttcctaatgt taagaagata tgaggataag
    14161 attgggttta tcgaatgtaa ggttccagtt cctgtttttt gcaggtttgg gaaaagtcct
    14221 gaaacccatg tcataacgct gtcacgtcat ctttcacggc gggataactg ccaacacgtt
    14281 atgaaacagg cgtgagaaca cgtcacaacg aaccgaaagc atatccgaca atcttcacta
    14341 acctatgcac agtgctaact ggggattgcc taagtccggc tcatttgccg gatatggtaa
    14401 cggaggctcc atagtagtcc gagaggggta atgaccctta catggcgaag ggagccagtt
    14461 agtaacatct aaaatgtaag aatgaaaggg aggagaaacc tcaatgaaac caacatctga
    14521 aattttagag cgtattgaaa agtgttccag cgaacacaaa gacggcgtat tcacaagact
    14581 ttacagatac ttgcttcgtg aagatattta ctacgccgcc tatcagaaac tttatgcaaa
    14641 caaaggtgca acaacacagg gtatcgacga cgataccgca gacggtttca gtgacttcta
    14701 tataaaagag ttaatccagt cactaaagga cggaacctac aaggctaatc ctgtaaggcg
    14761 tgagtatatt ccaaagaaaa atggaaaact ccgtccgctg ggtatcccgt ctttccgtga
    14821 caagctcctg caagaagttg tcagaatgat tttggaagct atctatgaac ctgtctttga
    14881 cagtcattcc cacggtttca gaccgggcaa aagctgtcat accgcactgc gtcagatttc
    14941 ttctgacttc acaggcgtgg tctggtttat cgagggcgat atacaaggtt gttttgataa
    15001 catcaatcac gaaaaattga ttgagatttt gagcaggaaa atcaaggaca gtcgttttct
    15061 gaacatcatt cgccagtttt tgaaagctgg gtatatcgag aattggaagt acaacgccac
    15121 ttacagcggt tctccgcagg gtggtatctg ttccccgata cttgcaaata tctatctgaa
    15181 tgagctggac aagaagtttc gggaaatcgc agaactcttt gataagccga ggtctgcata
    15241 tcagacactg gaatatcatg cggcaagcaa agaattaaaa agactgtctt actggattga
    15301 ccatacctct gatgaagcgg caagacaaga actgattgac cagcataggg cgcagaagaa
    15361 agctatgaga aatctgccct gcaaacctgc cgacaacaag aagtttactt ttgtccgtta
    15421 tgcggacgat tggctggcag gcgtgtgtgg cacaaaaacg gaatgtgagg aattgaaagc
    15481 cgaaatcgct gaatttctaa gcacagagct gaaattaact ctgagcgagg aaaagacgct
    15541 gataacgcac agctccgaaa aagtccgctt cattggctat gatatttgtg tccgcagaaa
    15601 tcaggaagtc aaaggtcaca gaatgaaaaa cggaacgtgg agaaaatccc gtaccctgca
    15661 catgaaagtt gccttgtcta ttccccatac ggaaaagatt gaaaaattca tgtttgcaaa
    15721 gaaagtcatt cgccaaaagg aaaacgggga gtttcagccg atacaccgag ccggacttct
    15781 caaccttgcg gattatgaaa ttgtggaaca gtacaatgcc gaagcaagag ggttatgcaa
    15841 ctattacaat ctggcttgtg attatcacac gttggactac ttctgctatc tcatggagta
    15901 cagttgctta aaaacgattg ctaataagca taaaaccagt atccgcaaaa tcattcgcca
    15961 gtataaagac gggaaaacat ggtctgttcc ttatgaaacc aaaacaggga caaagcgtgt
    16021 gcgccccgtt aaaatcgctg actgtaagcg tggcgaagcg agtgacatca tctatcagag
    16081 aaagaaattt agttggaaaa ccactatcag acagcggctc aatgccagag tttgtgaatt
    16141 atgtgggtgt aaagaagccg atttgtatga ggttcacgtt atacggaact taaacgaatt
    16201 gggtaattct gattgggaaa ccgtgatgaa aaagaagcgg agaaaaacgc ttgtcgtttg
    16261 cagcaagtgc catgaaagaa tacacagaca ctaatcaata cgtcaattac taatggggag
    16321 ccggatactt tgagaggagt aagtccggtt ctgagggagg gtgtaggaaa cctaccgcca
    16381 gcggcggcaa ggcgccttta ccctacccta cgattttctg gcaagcccgg agattgagaa
    16441 catctttgaa aactcggatt tccttgtgct gctctcgcag gcacagggcg accggcagat
    16501 tttagccaaa cagcttggga tcagccccca ccagctttcc tatgtgactc acaccaactc
    16561 cggcgaagga ctgctgttct tcgggaacac caccatcccg tttgttgacc gcttcccgca
    16621 gaacacagag ctgtacgcca ttatgaccac ccgcccggag gacaaaaaac aggaaatgaa
    16681 ccgggcataa cacacttcgg gccatgatgg cccgaggttt ggaaaggagg gatacatcat
    16741 cggaaagaaa ccggacaaac ggaattttca gaggccgggg ccgacggaga ataccggggg
    16801 caatcgcccc gcaccgtccc gacgcctgcg gtttgaggac gaggataccg gacaggacag
    16861 cccttcgggc cacaatggcc cgaagtccaa aagcggcaag tttcaagagg acagccgcaa
    16921 aagccgtccc tctgaccgca tgaggcagga ggatgaagcg ggcgggccgc aggataccgg
    16981 caaggcacag gagccggagg gctccgccga gaaggccggg agtaaaaaag acaagtacca
    17041 gaaggcacag gccaaagcgg agcgcgccgg ggaaaagctg ggaaaggctc gggagaagct
    17101 ggataaggtc gaggcgaaac gggcggcgca gaagccgccg gggcttgcga agaaagctgt
    17161 ccggggagcc cgtacagaag catggttcta tgtccacaac aaaatccacg aagttgagca
    17221 tgaaaatgtg ggtgtggaag gagcccataa gtcggaactt gtggccgagg ccggagcccg
    17281 gaaactgacc cggtatgcca aacgccgctg gcaggaacat ccggcccgga aggtggcaaa
    17341 gtgggaacgg aaggacataa aagcccgggc caatgtggat tttcagaaga tggcctccga
    17401 gcacccagag cttgccagca atccgctttc ccgtgtgcag cagaaatgga agctgaaacg
    17461 ccggtattcc aaagaggcaa aagcggctgc aaaacagggc gcaaaggccg caaagaaaac
    17521 cgctgccgct tcgggaactg cgacccggcg ggcggcgcag tttgtgaccc gtcatcccgt
    17581 ggcggtgctg atcctgctcc tgctgttgct gctctgtttt cttgtgtcgg cggtaagctc
    17641 catcttcccc acgcttggca gcggccttgc caatgcgtta tccagcacct cctacgcctc
    17701 ggaggatacg gacctgctgg gggtagacga ggactacaca gcgctggaaa acgagctggc
    17761 gcagacggta gcgaacatcg aaagcaccca tcccggctat gacgagtacc gctattccgt
    17821 ggacgagatc ggccataacc cctatgagct ggcgtcctat ttctcggcaa agtaccatgt
    17881 atatttccgg gaacaggtgc aggacgagct gcgggagatt ttcgaggcac agtatgagct
    17941 gaccttgacg gaggaagtcg aaatacgcta ccgcaccgaa accagcaccg acccggagac
    18001 cggggaaacc accaccgagg aagtctccta tgagtattac atcttaaatg tgaccctcac
    18061 aaacaaaacg ctgcctgccg tgatcctgcc gaggcttaac gaacagcagc gggaaatcta
    18121 caccgttatg cagcagctca agggcaacaa accctatctg tgggacggga tttacaacgg
    18181 cggcgaagat accggcccca actatgagat acccggcgag gcgctggatg acccggcttt
    18241 tgcggcgctc atggaagaag ccacaaagta catcggctgg ccctatgtat ggggcggctc
    18301 cagcccatcc acctcctttg actgttcggg ctttgtctgc tgggtgtaca cgaccagcgg
    18361 catccacaac ctgccccgta ccacggcgca gggcatttat aaccagtgcg ctatcatttc
    18421 cccctccgag gcaaagcccg gcgatattat ctttttcaca ggaacctatg acagccccgg
    18481 ccctgtgtcc catgtgggga tttatgtggg cgacgggatg atgcttcatt gtggttcgcc
    18541 catccaatac gcaaacatca attcaagcta ctggcagaca catttctatg ccttcgggcg
    18601 tttgtgagcc agaggaaagg agtccttgca tgaacaagat cgacaagctc gataaggaac
    18661 tggaaaaagc ccgggagaag gctgccgagt ggcaggccaa aatccgggag ctggaaaagc
    18721 agaaacagga ggaagaaaac agccagatcg tgcaggcggt gcgctccctc aaactgacgc
    18781 ccgcccagct tatggctttt ttgaatgacc ccaaaaacag ccttaccgct tcgggtcata
    18841 ctgacccgaa gccggaggta cccggaaagg aggacacagc ccatgaagaa aattaaatac
    18901 cgcaggctgg cggcgatggc tgccagcctt ttgtgttgcc tgatctttac agtcccggcc
    18961 tatgcacaga gcagcgagcc gcagccggag acagctccct ccccggcaga aaccgaagca
    19021 gagccggaaa cccagaaccc ctttaccccg gacgggacgg gaaccgtggt ggacaacgcc
    19081 accgacgagg atggaaagga gttctacacc atcaccacag cagacgagag cgtgttttat
    19141 ctggtgatcg acaaacagaa aaccagcgag aacgtctatt tcctcaatac cgttaccaca
    19201 gacgaccttc tgcctcttgc tgaacagggt aaggaaccgc ccaaagaagt gaccccggag
    19261 ccagagccaa agcccaccga acctgtggag gaagtaccgg aacccgagcc ggagaaaaag
    19321 gacagccccc ttctctctct gctcctgatc ggtgcggtgg tgctggccgg cggtgggatt
    19381 ggttactatt tcaagattta caagccgaag catgaggccc cggatttgga agatgattac
    19441 tgcgaatatg aggacgggga gctggaggag atcgcagagg aacccgagga cgaagatacc
    19501 ccgccgtggg aggaagatac ggaggaatga gaatgaattt tacaagcagc ccttttgaac
    19561 gaatgatgaa ggaagtaccg caccccggct gggacaatga cgacccttgt aagggatgcc
    19621 gttactacaa ggagtgcaaa ggaaagaaaa aacagtgccg gaagaagttc cgggcgctga
    19681 tcgtggagcc ccgcccttcg ggccatcgtg gcccgaagtc ttaaatggac gcccgggagc
    19741 tgacccgtga cgagaagaaa aaaatccgca ctctggtcac ggggatgtgc gccaactatg
    19801 accgggaaag cggtctgtgc cttcctctgg actgtgcctg ctatatgctg cacaagtgct
    19861 ggacaggggc gtactgccgt tacttccgcg aggctgtcct gccccttgac ccggagcttc
    19921 aagcggcgct gacaacggaa ggcatctccc cggagctgcg ggcctgtgcg gtctgcggaa
    19981 aggcattttg gcccgagggg cgtcaagcct actgttccga cgcctgcgag gctgagggga
    20041 accgccggaa aagccgggaa cgcatgagga aaatgcggga gaaaaggccg ggcggcggtt
    20101 acgatttgcc gcccccaaag gcttgatatt ccgggctttt ttgagggcgt ttccggggtg
    20161 ggaatatctt attacattcc tccccggtct gccctcttat ttcgtaacat ccagcacttc
    20221 gggccatgct ggcccgaggc acagaaagga gggatctctt ggagacagta caaggatatg
    20281 tgattttgaa agctgccacc tttgaaaccg gacacggctt tgcgctgggg cataatccgg
    20341 gagcgccgag cccctttgtg acctggcagt ttaccgaggg ggaaaacggc catcgggatt
    20401 actactgggg ccgctatgga accagtcagg cgtgggcgca gagggacttt gaccgccggg
    20461 tggacgacta tcagcagctt taccatgcgg cggtcaagca taccgagctg ggaccggagg
    20521 gcgtttaccg ctattattcc acccagcgcc ccgtagacat aggcacctac ccgaagctgc
    20581 cggataacca gcctctttcc atcgtcaact acgatgacga caggcggcgt cctgtggcag
    20641 acagcaggct aatggcatgg ggcgaactga cctacgcaaa gccgctgacc gaaaagcaga
    20701 tggaggatta tgaactgaaa ccagcaccgg gcaatcctga ccgggtgcgc ccatccatta
    20761 ccgcccggct caaagaagga accagagggc aggaaccccc gaaggagccc ggcccgaagc
    20821 ggagccataa aacccgtgag gaacgataaa ggaggatcgc catgcacgaa aaagacaact
    20881 atctgaaaac cgccgagctc tccacggagc agaactgcaa catgatcgac ggcgtaccca
    20941 acaacacgcc catccaaccc acgcccccgg agctggacgc aaaaccgctt gataaggtaa
    21001 aggaacccaa agagcgccgg aaaggccggg agctggaacg ctgatggaga accgcaagcg
    21061 gaatgtccat ctgcacgtca tggtaacgcc ggacgagctg gcagccatcc atgaacggat
    21121 ggctgaggcg ggcatttcca acgccggggc ttatgtacgg aaaatggctc tgaatgggta
    21181 tatcctgcac atcgaccttg cgcccgtaaa agagctgatc tctctgcaac ggcgctgttc
    21241 taacaatctc aatcaggttg ccatacacgc acatacctat ggcgtatacc cggaggaaat
    21301 cgacggattg aagcgggact atgaaaagct gtggggcgag gtgtcaaagg tgctgcggga
    21361 gctctccgag ctggtggcaa agtaagacga gggcggcagg cttccgttgc agggcttgcc
    21421 gccctgttct ttttttacca cttcgggcca gtatgacccg aaggtttggg acaggattta
    21481 ctaatctttc gcaacattta tgtccccggg caggcatgat aaaatagagg tagaaaggat
    21541 agataaagga ggttttgaag atgaagatac tgctgaaaat attgatcgct ccctttgctt
    21601 tagcgttgtc ccttctggcg gctctgctgg tgttcctgtt tgatatttgt gccgtcctgc
    21661 tgacgattgc ctctgtgatc ctggcggtgc tgggtgtcgc tctttttttc acgccgacgc
    21721 ccataggagg gattgtattt ctgtttctcg ccttccttct ttcgccgtat ggactgcaag
    21781 cggcggcggg ctcccttctt tgggcgctgg acggaggcaa atccgctctg taccggtttc
    21841 tggcaagtta agcagcctcg ggccatactg gcccgaagtc ggggcggtct gaatgggccg
    21901 cccttttctc atggaaagga gggatgattt ttggctacca caactttgtt acagcgccat
    21961 gcgggcgaag gcgaaacgat tgctgaggct atccgggatt gtctggacta tggcaaggac
    22021 ccggagaaaa cagaaagcgg aaagtatatc tccgcttatg aatgtgatcc ggccaccgtg
    22081 gcggacgagt tccttttggc aaaggccagc tatgccgcta tgacgggccg ggaacagaag
    22141 aaagaaaata atgtgctgtg ctatcagata cgacaatcct tctatccggg cgagatcacc
    22201 ccgaaggagg cgaaccgtat cggctatgag ctggctatgc gctggacaaa ggggcggcac
    22261 gcttttatcg ttaccacgca caccgataag cagcacatcc attgtcacat ttattacaac
    22321 tccaccaccc ttgactgcac ccggaaattc cgaaactttt ggggctccag cttcgccctt
    22381 cggcggctct ctgacaggct gtgccttgaa aacgggctgt ccatcgtgga gaacccgaag
    22441 ccccggagca agggtaagta tcggaattat ggagaatggc agaaagaacg aaaagggccg
    22501 ctttcctatc aggacaggct gcgcctcgcc attgatactg tgctggcgga gcaccccgct
    22561 gatctggacg aatttctcaa tctgatgaag cgggccggat atgaggtcaa gacggttcgg
    22621 ggcggcggta tcagtttccg gctgaccggg cagggacaag aacgctttac ccgtctgcgg
    22681 gcctccacgc tgggggacgg ctacgacttg caagatgttc tggccgccat tgagggcaaa
    22741 gaaaaacgcc ccggacattc ggagcggaaa atcagtctgg cggtggatat tcaagcaaaa
    22801 ctggctgccg gtaagggacc gggatatgaa cgctgggcga aggtatttaa cattaagcag
    22861 atggccgctg cccttgccta tatacaggac aatggactga ccgattatga gcagttggca
    22921 cagaaagcca ccgaggcggc agaccgtttc catgccattt ccgagcagat caagcagacg
    22981 gaacaggcca tgaaaaccaa tgccgggcta aaggccgcaa cggttcagta tgccaaaacc
    23041 cgtccggtct ttgagcagta taaggcgacg aagtatagcc ggaaatttct tacggagcat
    23101 gaagccgacc ttgaactgta ccgggctgca caagcggaaa tgcgctctct gttgggcggg
    23161 gcgaagctcc ccaaaatgga tgtgctgaag gtagaaggcc gtaagctcac agcaaagaaa
    23221 aaacaactct atggagaata tcaaaaggca cgacgggata tgcaggaaat cgtcacaatc
    23281 aaggcgaaca ttgatactct gctgggctac accgagccgg gcagaaagca tgaaaaggag
    23341 cgttgagatt atgaaaacaa ggagcaaagc gacgctgaca cttcgggcca tgttggcccg
    23401 aagatacggg tttggggcga gccccaacaa gccgcctttg tgccacttgt ggccacagag
    23461 gcattgcttg ccacttagcg gcagccccta aaatggcgca aaaaaacaga ggcccttatt
    23521 ctggaacctc cgtttctctg gctttcttaa taccctcggc tgtggcttct atcacgacaa
    23581 gctctttttc attcatggag tttagcagca catcaatttg ctttctgcaa gagcttgatc
    23641 ttgcccctcc gtccgcatga ataaactggt caactgaaac gtcaaacatg gtaatgagtt
    23701 taacaaaaag gtcgaagctg ggatactgac ctttgttctc aatattcata atggtacggg
    23761 agtcacggtc tactaattcc gcaacatagg cttgtgtcca gcccttttct tctctggctc
    23821 gtttgagagc cgccccgagg ccgtgaaagt caaaccttct ttcatcttgg ttcattctca
    23881 tatcacccta tatcattgta catttcgggt tgaattatga gaatgtaatg aaatttgatg
    23941 tgaggtagta ttttatttct tgttttcgtc tgtctattat aagttaatat atttctattg
    24001 gaataacaac ttccacgctt aatccgtgcg gggcagtatc agaaaattga attgtgcctt
    24061 gatgtatttt cacaatctgc cgcacgattt taagacccag cccatgctcg atttctcccg
    24121 tttgcttttg cgtactggaa atatctttgt cgttattcaa tgtgtttagt tgcggctcac
    24181 ttattccaca gccattgtct attacagaaa aggtacatgt tctatcacag cttcctactg
    24241 atacttgtat cctacacccg tttggattat ggacaataca attcctgatt aaattgcata
    24301 acatccggtt tagtaaggat tgatcgccaa taatagtgat agcttttccg ggattatctt
    24361 ctgatagctc taattcatat ttgtctgata ggccatcatt caaaacttca cttacggctt
    24421 gcctcgccaa ttctaccggg acaagtgttt gcttttgcat aggctgcatg gaatattcca
    24481 gttttgtagt caaattcaaa ttctcaacta aattctttag tttttcgcta tgtttgcgga
    24541 taatttcagc ttgctttctg gtggtttccg gcaaagaaga agtttcttca atttcgctgg
    24601 cataccctaa aatcatagaa agcggcgtgc gtatatcgtg agaaataccc ctgatccatt
    24661 cggcgcgggt gttatctttt tttagtaagt aatcaccggc tttattcaaa ccggcattga
    24721 tttctgctaa ctccccgttt tcttccagat gaacgggatg cccggatgaa agatgtcgta
    24781 tgccattcaa aatcggctcc atagcttttt ctatccggtg ggcatttcgg acaaatagat
    24841 acaacattaa caaaacattg atacagacag cagctaaaaa tccaaacgct atcggccgaa
    24901 catattgact tatgatggaa gtgtagtaat tcgttacata tccctgcggg aaaccaacaa
    24961 ctaaaaggct gtcttgtcga ttccaaatat taactggata actgttaaga taccaacggc
    25021 tgaacatagc aacgtccgtt gtggaatagt ggcgcggcaa ttcttccggc acattcattt
    25081 cccaaattat gtttccattt tcatccaaaa tcatggccca tgcgcaagca ttgtttaaca
    25141 tgtcctctgc ctgtatatcg gcattgaatt gcccgttagt ttctgtgata tgatttgaaa
    25201 aatcttcaat cgggaaattt gagtttttta cattccctaa ataggcggct gcaaagaaag
    25261 aggctactaa caaaatattg acgaatagaa agagaatgat gatactgact attgaaaata
    25321 tatagcggcg aaaaaatctc tctgatttgt tcatgtttgc acccccttaa tattcagacg
    25381 atacccaatt cctttaattg ttacaagcgc aactggtttt gaaggatttt cctcgatttt
    25441 ttctcttaaa tgtcggatat gagttgctaa tgtactttcg tatccttgcc agaaatcacc
    25501 gcatattgct tgacataatg cgcctgtggt aacgatcctg ccttgatttt catacagttt
    25561 ttcaaaaagt tgaaactctt tggctgtcag cgaaatattt tctccgcttc tctggacgat
    25621 tgctttttcc agatcaaccg tagcagcgtc aaagaacact tttcgctctt tctccgggta
    25681 aacgcgtttc aatatagcct gtactcgaaa tagtaattct tttggtaaga atggttttaa
    25741 cagatagtcg tctgcaccat tttcaaagcc tacaaattta tcttcggcct cgccacgagc
    25801 tgttagcatt aagacaggaa ttttgctggt ctttcgtagt tcttttaatg cagcaaaccc
    25861 atccatacca ggcatcataa catcgagtat catcatatct ggttcttgtt cctcacacat
    25921 ttttaaggct gcctcgccag acgaagccgt caatacttgt gtaaatcctg ctcttgtaaa
    25981 aatagatttt accatctcca acaaatctgt ttcatcatca actacaagga ttcttttatc
    26041 gttcataagg caccacctac tattatataa agtcatagtc tttctttacc ttttctacag
    26101 gtttgactat gctattttaa gatactgtgg attggttatc acctcctacc acttgatggt
    26161 ttaaaaaagg ctccaaccac agtctctttg tttatttgtt aatcagttag ttaccgcatg
    26221 acggtttatc aagaagtagg ttacgattgc aaggagccct gtggatctta agacagtatc
    26281 aatactttat atcaggaggt cctatatgat ttacgtagga attgatgtcg caaaagataa
    26341 gcatgattgc tttatcacaa actctgatgg cgaagtcctt ttcaaggctt ttaccatcaa
    26401 aaacaatctc gatgggttcg acgagcttta tcagaaaata gaatccgtta tggaagatgc
    26461 ttctaaagta aaagtaggcc tagaagccac tggacactat agttacaatc ttctcggata
    26521 tctgcttgat aaaggtctgg ccacctttgt tatcaacccg ttacatacta atctgtacag
    26581 aaaaagtcta agccttagac agacgaaaac ggataaagtt gatgcccata caattgcttc
    26641 tatgctaatg tctgacgtga acttaaagtc ctactcagac acatcgtatc acaacgaaga
    26701 gcttaagtca cttactcgct atcgttttga taaagttaaa gaacgcgcga agcttaaaac
    26761 atctatatcc cgtcttgtat gtatcctttt ccctgagtta gaaaagcttg ttccaacact
    26821 tcatcagaat tctgtttatg agttactcta cgaatttcct ggtgcaaaac aggtagctaa
    26881 tgcacatctc acaagacttt caaatcttct tgaaaccgca tctaaaggcc actacacaaa
    26941 agaaacctct atcgctttta gagaggctgc aagaacctct atcggttcaa atatgccagc
    27001 taaatcgctt gaattaaagc acaccattaa gctcattaga gagctagatt ctgaaatcga
    27061 agagattgaa aacgagatta aagtcatcat ggatgaaatc aattctccaa tccttagcat
    27121 tcctggaatc agctatcgaa tgggtgccat gattattgct gaaataggtg actttagcca
    27181 attcgactct ccagataaga tccttgctta tgcaggaatg tcgccttcta cctatcaatc
    27241 cggccagtta gataactgtt atgccagaat ggaaaaacgt ggttctagat accttagata
    27301 tgctctgttt aatgcaaccg catatgtttg tctatgggat ccaacctaca aggcttatct
    27361 tgccaagaaa cgagctgaag gcaagcatta ctatgttgca atgtctcacg cgaccaagaa
    27421 actagttcgg ctaatttatc atctcgaacg tactggacag caataccaaa aagcaatcta
    27481 attcttccaa catcatatct tcttgtcaga gcatcatact cgatgctcta tttgtcatgc
    27541 aattttcaag gtactgattg cgatgaataa gtcatcgcca ctatactcaa aacatttctg
    27601 tttttgaaac tatttattga ctttctttaa tagttagtct tttatttgtt gctcttatta
    27661 tacctcaaga taggtaaaat gcctattctg accttgaaat ctcaaagtaa actcaaggtt
    27721 ggctcaatgt tatctcaaag ttgatatgtt atgctgagta cacaaacaaa aaaggaggca
    27781 taagtgatga acgattacat tattgaaaca cataatctga caaaaaagta tggaagtcag
    27841 gccagtgttt cccatctgaa tattcatgtg aaaaagggac gtatctacgg gctattggga
    27901 cgcaacggtg caggtaagac cacgaccatg cggatgttgc tgggactgac ggctcccacg
    27961 tctggcgagg taacgatatt tggaaagccc tttcagggca acgaaaagaa tatcctgccc
    28021 cgtataggct gtctgattga gtcgccgggc ttttacccca atttgacagg aacggaaaac
    28081 ctaaaaatct ttgctgaact tcgaggatta aggagccccc aatatatcaa aaatgcattg
    28141 gaacttgtta atctacctta ccgggacaag aagttgtttt cccaatattc tcttggtatg
    28201 aagcagcggt tggcgattgc tctggccgtc atgcataacc cggaactttt gattttggac
    28261 gaaccgatta acggcctcga ccccatcggc attgcggaag tgcgagattt tatccgagaa
    28321 ttgtgtgata ccagcggtaa aaccatttta atttccagtc atattctttc ggaaatctct
    28381 ctgcttgcag acgatgtggg aattattgac cacggcaagt tgctagaaga agaaagtctg
    28441 aaagaattgg aggcgaaaaa cgccaagttc atccacttct gcgtatcgga tgcacaaaaa
    28501 gctgcgaaaa ttattgctgc aacatttcac agcaaagatt ttcgccttac agatgccaat
    28561 aatctgtatc tctacgatat gactctgcca gtggaaagga ttaaccgttc ttttgtcgaa
    28621 tcaggaattg agattcaaga agtccatctt tgtgaggata ctttagagga ctacttcaaa
    28681 aagataacag ggggtgaggg gattgcttaa cttgattcgg tgcgagtttt ggaaactgaa
    28741 aagaaaaatg ttcgtgcagt tggtattggc ggcatctttt ctgtttccaa tcccgctaac
    28801 tgccattatc tattatctga acacggcgca gaacaaatac gctacaaaag cagcagcttt
    28861 tgacgccctg tggcaatccg taatcggttt tggtatgttg ctacttcttc cctgcatttt
    28921 aggaattgtt gcagcactcc tcttttttat ggagcgagat aacgacacct ttaagaacct
    28981 acggacaatt ccgataacaa gttcacagat ggttatcgca aagtgcattg ttttattatt
    29041 gcttagtatt gcgttttgta tatcttcgac tgtggcctct atcctttgcg gttttgtctt
    29101 tttcggagtg accgatattt tgtttaaggt attgttttcc gctgtttacg gattattgat
    29161 tgcgattagt gcattaccgc tgatattgct gattgtattt ttcagcagat cctatatttt
    29221 ttccattatg ctttgtgtgt tttatagtgt attcaacctg ctctcaacat tttctatgac
    29281 agcacttcca aaatggcttg tacttactct gtctacaccg agcattatgt tgtggggaac
    29341 ccagcaaatg tcttctcgta ctgctataaa cgacacagag gatttgcaga acttcattca
    29401 aatgggattg ataccttcta ctcctcaact aattataact ttgggggtaa ttggggctgt
    29461 ttcacttctt ctggcggtct atctttataa gaaaaggagt gagtgaaatg gtaacaatta
    29521 taaaaaccga gtttatgaag ctgaaaagat attttattgt ctggattggc gtgtccctca
    29581 tgcttcttac agttctgcta accttgttta ctacaatggc cgatgacaga atggtatggg
    29641 actaccaatt tctttttgaa caggtagtta aacattttgt aacaatgatt ttcccaatgt
    29701 gtatcacgct gattagtggg tatatgattt cccgtgaata tacagacgac acattaaaaa
    29761 atattgaaac attacccatt tcatttagga aacttctggc gggtaaattg attgtttctg
    29821 caattctgtc gtttttctta ggaattgtgt gctttgcttt cacagtcgtt gcaaatttta
    29881 tcatgggata tgatggattt gctttgattc ctgcgcttac aggattggtg caaatggcat
    29941 tgttaggatt cttcctttac ttgacaatgc tgcccattat tgttctgaca agcagatata
    30001 aagggagttt ccttgtaggg gttatcgtcg cttttgtata tgggtttata ggaatgtttg
    30061 caaatgggac tttacaatct atatatcctg tctccgctgc gcttggttta atcaattatc
    30121 gagctggcgc agaaggagtt atgtggaata aaggactgtg ttttattagc attctgatta
    30181 tgtgtgctat tggaattgct ttgatgtttg taaaacagaa accagaaaag agagaagcta
    30241 aaaaaacaca gcacacagca cctaaaaagg gctggtaaaa gaaagagagg taacctttat
    30301 gagttctaag aaaaaaatag gaattgtgat tgtcgttgct gtactagcaa ttacttttat
    30361 gactgtagct tttctcacta cgcaaaccaa tacagactac tatacgcaga ttgataaccg
    30421 acgggtaaca gatattgcac cgcatggtgc gatgaactat aaatacacat tggttgctta
    30481 tgatgaaagt ggtaatgcga aagacgttac ttttgaaaca agcaaagttc tcacagatgg
    30541 tgcgtatctg tgcttgaaag tggctcccat cagaggggtt gtaacatggg ctgaaatgca
    30601 gtttgatgaa ttaccttctt ctgttcaaga aaaatataaa gaataacccc gcaggacaat
    30661 aaaggtgcct gtaatgaaat atatttagat cagctgccac acgacggcaa aagaaaaaaa
    30721 gccgtcgtac agcagccctt ttgacacgcc catacgacca gcggcggctt tgattttcag
    30781 agccgccgtt tttttcgttt atcctaaacc aacgacgacc taacaccgtg taaatccacg
    30841 gcggcatata aggaggattg ccgccgtgtc tatttttgcc tcttttcata gtacccatga
    30901 cctacatcaa ttaaaaaaag catagtgccc cctccgggtt attccggcta tgtatgcgaa
    30961 atttgttgga accttaacta ttatgtcatt tcatgcgttc gtgcggcttc atgccgcccg
    31021 ggcgctttta tgttcttatg gggctgcttc gggtcatgtt ggcccgaagt cattccccgg
    31081 tgccgctccc cgccgccttc gtttcggtca ctcgtcaacc aacaaccgaa acggaggtca
    31141 atatggctat tatcaatttg cgggactatt acccattcta tacatcggat tgcttcatgg
    31201 aagtatcgga agaagttgca gaaatgttca aagagtttga tcgtaaagag gctgcctacc
    31261 ggctgcgtac ataccgccac aaagcctact attcccttga tcgggatgac gggctggagc
    31321 atgaagctgt ctttgttgcc ttatctccac acgaactgta tgagcggaaa gtgaccatgc
    31381 aggaacttca cgcagcgatt gccagtctgc cggacaaaca ggcaaaacgg atttacgccc
    31441 atttcattct cggcatgacc aaacaggaca ttgcccgggc agagggcgtc catgaaaaag
    31501 tggttcgtgt cgcaatcgag cgaggtcttc ggcgcttaga aaaaattttg aaaaattctt
    31561 tgtaatgcgt accgatttag gccggaaaat gaaatggttt atgagaggca aaacacttcg
    31621 ggtcacaatg gcccgagttt cagacaagcc tcatgcagat tgaaaattga ataaaaagac
    31681 acccggatac gaagggaaac gcgctgtgtg acagacccgc catgaccttg gatttcagag
    31741 aatacagtct ttgtaaaact gagcgagcga acaggttcat gccataggtg gggcaaggct
    31801 ggctccaccg gaacaggcgc agaacccgga tacttgcgtt tagtcacagt ccgagcgttg
    31861 aagcggcctt gcaagccgtg agccgtcgca ggcaatgaga acgccttgcc atcagaatgg
    31921 ggagagttga aatactatgg ggcatgaagc ctatgtccgt ccggtgtgtc tgaaatgaag
    31981 tgaaaaccta tggggagccc ccggcaatgc tgtctgatga taggccaacc gatccggcgt
    32041 ggctccccat cttttcaaaa aaggagcact ttatgggaaa tgcgtttgga atgatccccg
    32101 gcttggaacc ggacgaatgg agcaatgacg cttgccgtgg ttatgtcatt atggcaatgg
    32161 aggactgcgg attttcaaag aaggatatac ggcgtgttgt ggggcagctt tatgaagtat
    32221 ttgacctcaa cagcgtggag gacgccaaag aaaagtacca ttccagtcct tactgacctc
    32281 gggccacatc gacccgaagt ggagaaagct caaagggcgg cacctgcggg gtgccgtctt
    32341 ttgacatttc cccacgggac aagtgggaaa ggctggcata tacacgcact ttcgcaaagg
    32401 aggttttcaa tgacgcaagc tgtcacttat gaacgagaaa caaagtctgt cgcatttcaa
    32461 gggaagatca ttgtgctgga aagcctcacg ccggtacttc ccccgaagga gaaagaacag
    32521 cgcaaaaaag aaattgagcg ttgtctttac gaggtgttca gcaaatatgg ggacagattt
    32581 ccctaatcat tcgcaacatt gttgtccggg gctgctgatg gtataatata gttgtaaggt
    32641 tggtagctcc attccaacaa ggaaaggagc ccaatatgga atttatcaga gaagattgta
    32701 tttacgcaag acagtcagta gaccgcaagg acagtatcag cattgaaagt cagatcgact
    32761 tttgcaagta tgaattgaaa ggcgggagct gccggatatt caaggacaaa ggctactccg
    32821 gtaagaatac ggacaggccg gagtttcaaa agctgctggg cgagatccgc aagggaaagg
    32881 tccggcgggt tatcgtgtac aagctggacc gtataagccg ctctattctg gactttgcaa
    32941 cgatgatgga gctgtttcaa gagtacgatg tggagtttgt atcctctacg gaaaagtttg
    33001 atacttcgac cccgatgggc cgggccatgc tgaatatctg cattgtattc gcccagcttg
    33061 aacgtgagac aattcagaag cgtgtcacag acgcctacta ttcccggtgc ctgaaaggct
    33121 ttcacatgag cggacaggca ccatacggtt atcagttaga gcctactgtg gtagagggta
    33181 tccgcacaaa gaaaatggtt gccgaccccg tagccgccga ccatgttcgg ctgatgtttg
    33241 aaatgtacgc tgaaccggaa acctccttcg gagatattac ccgatacttc gaggaacatg
    33301 acataaaaat ttatggcaaa tccatgttcc gtacatttct ttcccagctt ttaagaaacc
    33361 ccgtttacgc acaggccgat ttggagctgt acgaattttt caagagtcag ggtgcagcga
    33421 ttgtcaatga cgcttctgac tttgccggaa caaacggctg ctatctctat caggggcggg
    33481 atgtgaagga ggacaaggac aggtgcttaa aagaccagat acttgttatc gctccccatg
    33541 aagcactcat ttcctctgac acatggctga aatgccggaa aaaacttatg gcaaatacca
    33601 ccttccagca gggacggaaa ccgaagaaca cttggctggc cggaaaaatc aaatgcgggc
    33661 attgtgggta tgctctgaaa gccacccatg taccaaacag caccggatat ttccgctgta
    33721 ccaaacggac ggaaaacaaa ggctgcccgg gctgcgggaa aatccgtaaa gaagaatttg
    33781 agcaattcat tttctcggcc atgcaggaga agttcaaaga ctttcagata ctccacggca
    33841 gggaggaaaa agtcaatccg aaactgaccg cctatcaagt ggagctggca caggtggagg
    33901 cagaaattga aaagctgctg gatacgctga ccggagccaa tgcgacctta cttgcctacg
    33961 ccaacaaaaa aattgaagaa ctggacaccc gacgccagac catttcaaag gcaatcgccg
    34021 aattgagcgt tgaaaccata tctccccagc agataaagaa gttatcctat tatcttgaca
    34081 actgggacag catagatttt gacgacaaaa gaaaagccgc cgatggtttg atctctacga
    34141 tcaaggccac cagcgaccat gttcagatag agtggaaaat ctgacatttc cgctctatcg
    34201 cctctcattt ctattttatc ttgtttgtac cccttgtaca ccgatgtttg ctattcctaa
    34261 tgaaaaatta ctatttaata ggagagcaaa atgagctata aaaaagcaat acatatatta
    34321 ccagaagaat tacttgaatt aatacaggaa tatgtagatg gtgaatgtat ctatattcct
    34381 agaaaatcta ataataaaaa agaatgggga agtaatacac ctactcgtga ggagttgtca
    34441 attagaaata tgcagattta cgaagactat caagttggat atgatttgca atatttatcg
    34501 gaaaagtatt atttatcttt gaaaagtatc caaagaattg tattacaaga aaaaagaaaa
    34561 gatatctaag gatgagccag tatagtttta taattatact ggctcatttt cttttgataa
    34621 aagtgtttga ggtatagttt cttttaagtg gatgatataa tttaagtaat ttcaataact
    34681 tatacagtag ctaacaaagc tggttgtaaa gtatctaata agaagtagta gctttttata
    34741 tgtttgaaat taaaatttat taaaattagg aggtcttgtt ttgaaaaaaa tacttgatta
    34801 tattccacct tttcataact ttctattaat ttagtaatct tatagaaagg aaattgcatt
    34861 tatgaaaaaa attacttggg aggtggaata tatctcccag ttacctgtaa ttaggtattg
    34921 taaaaaatgt ggaaaaaaga ctgaatatat ttgttcaggt ttatttaggg ttaatgctca
    34981 acataaatat ttggatatat ggttaatata caagtgttca aattgtgatt caacatggaa
    35041 ctcaactata tattctcgtg ttaatccaaa aagcttaaat ccagaaatac tagaacaatt
    35101 ccatacaaat gattataatc ttgttaagca atatgctatg aatatggaac ttttacatag
    35161 aaatggagca gaagttggta ttccagagta taaaatagta ggacaagaaa ttaatattaa
    35221 tgaccttaca caagttcata ttattagtaa atatccatgt cagttaaagg tatcaacatt
    35281 attacgtgag aaactaggtt tatctaggaa ggtatttgac gaaatgttgg attgtgggat
    35341 tattagaagt aactcaggag ttgatttaaa gaaaggtaaa ttaaattatg aaattatgtt
    35401 agatataggc aaggagaagc aaaagtcttc aaattgatta gaataactaa cttaatatat
    35461 tttcaggaac atcatttaaa aaaatagtga ctttaaaaaa tttaaagcca ctatttttta
    35521 atatgtttta tctatataac tattattttt ctaggttttt taatatttcg taatagtgtg
    35581 ttgtggctat ttcacttgac tcgccttttt tacaaaaaga taatttagat agttttggaa
    35641 ctacacgttc aacgacaatt ttattaccta tcattttaga ttcaagtatc ttcaatgctt
    35701 ctaaaaatct attatccttt tttgcacgat tataaaatga taaaacgtag acataaagga
    35761 ataggttata attacggaaa ggatattcaa cttgcataaa taatgtgcca attccatagt
    35821 gacaagggcc gattggtttt ttaatagtcc aatggtcaag tagaaaatct acagctttgt
    35881 caagttttga ttctttattt agataattac taaaacggaa tgcatttaat atattaagag
    35941 ttggtagtgg atttgagtat tcagtttctg gacctcgacc aaaactaaat ttattgcaac
    36001 gccacccacc atcagtatac tgtgtatcta aaaaatgttg aaacgttttt tgcattctaa
    36061 tgtcagatgc atatcccata tgacagagta cgttagcagc atttgcagta tgacaagggt
    36121 aaatacctcc atttggatat agtttgaaac gtccatcttc tcgccatgtg ctaaatatca
    36181 ggtcagcaca ttctttaagt aaagtatcat cagggttcac acctaattct aatagataaa
    36241 gtacactatc aagagtagag aaaggagaac cttttattaa acgcttatca ggtgtagtcc
    36301 aatagtctgc accattatca tacctgtgtg ataatattat ttcaatatct gataaagatt
    36361 gattttctac attcattttt tactccattt catattatat tctattaaat ctatcaaaaa
    36421 ctttattata atattattta tatttataat tgatttattt tatttaaaat tgcatttata
    36481 gctgtatcag gaacaactgc taaatcttct gcattgaagt aataaactat attagaatca
    36541 aataatatat ttgaatttgg aggaaattca tcatctccaa accaaagtat aaataccata
    36601 gatgtattac ccatatatgt aaacctataa gctaaatctc ccatgtttac tttttcagct
    36661 ccaattttct ccataactaa cttatatttg tctatttgag atgtaaaaac ttctgaaagc
    36721 ctatatagag tcctgtttaa aaaatttgga taataaacat tgcctcctgg tatttcttta
    36781 aaagttacat atttattagt tctaggaata cctttagcat tgattaaata tctaactatc
    36841 aagattttaa gctcaaagtt attgcaaagg ctaccattac tattccaaac ttctccactt
    36901 ggatagtcta taatatattg tctatcaaaa aaattgagtg tgaatctatt taaatcatta
    36961 ttgaaaggta tattacttaa attactaatt gtatgagggt ctaattcttt gagttttttt
    37021 cgaacatagt caaatggaac attataatta ttttttgtta tcttttcatc cataattttt
    37081 tcctccaatt taaacaatta aaagtatatt tataaaaagt tttcttatta ttaattttat
    37141 tatatattca aaatattaca ataataaatt gttaaataaa tttttatatt taagtaaata
    37201 cgtgtaaaat gaatataaag tttttgaaat ataaagtttt tgtatctaaa atattttatt
    37261 tgtaaaatag taattaatta ttgtaagcta taatatgaga gtattatggt taatgtgaat
    37321 tttaaagata ttattaaata tataaattag aatactatta aacggataaa tggaggagtt
    37381 attatgtcta agatattagt agtagatgat gagttagata tgttaaagtt gattgaaaat
    37441 gtgttgaaaa gagatggaca tgaagtaaag gtaattagtg atgtagaaaa agctatgaac
    37501 atggatttta attattttaa tctaataata ttagatgtta tgatgcctaa gatggatggt
    37561 tttgaagtat gtaaaactat aagaaataag gttgattgtc caatactgtt tttgacagca
    37621 aaaaatatgg aaagtgatat tatgtacggt cttggtatag gtgcagatga ctatataacc
    37681 aaaccttttg gtgtaggaga attaagggct aggataactg ctcatttaag gcgagaaagt
    37741 agagaaaaaa agaatttatt aactatctca aatgttcaat ttaacttttt aggcaaagaa
    37801 gtttcaattg atggtaaaaa aattaatttt actaaaagtg aatatctaat atgtgaattt
    37861 ctagcaaaaa ataagggaca agtattttca aaagaaagaa tatatgagtc ggtatatggt
    37921 tttgatggag aaagtgatat aagtactatc acagagcata taaaaaatat acgaagcaaa
    37981 ttaaaacaat atgatattta tacaatagaa actgtatggg ggatagggta taaatgggtg
    38041 taataagtat aagtgttgtt tagtgaaaat attatatata tctacaaaat gtttgtaaaa
    38101 tattaatcta tatattaaaa tttaatttat agattaatat tttttgtaaa tgacaaaatt
    38161 gtaaggtatt ttgttataaa atattaagat tcatagtgta tactaataat atagtattta
    38221 gtataaatct tgagcaaagg atggttattt ctaatggata gactaaagaa tattagaaag
    38281 agtttactac agtatacatt tttatttttg ttaataggta taacaattta cctagtgttt
    38341 aaaacattgg atattaaaat gctatcaaat gttatggtta tggtagataa gaagttttta
    38401 ttcataggtg ctttggcaat aatgtgtcac ataatgcttg aaggagtagt aatgaagata
    38461 attatagaaa gcactcacaa agttaatatt agatttattg gttttaagct agccattatg
    38521 ggattttatt ataatttaat aactcctttt gcttcaggaa gccaaccagt acagatatat
    38581 gtacttaaaa aatgtaaaat gccacttagt aaggcaagtg cagttgttac caataagtct
    38641 attatcttcc aaatagtagt tactttttat tgtacaatac ttgttttaat gaattttgct
    38701 atgttagaaa aacaaatgaa aatgattatg cctctagtgt ttctagggat agccattaat
    38761 tcatttgtct taattatgat aatattagtt attttaaatc ctaaaaaaat aaaatatttt
    38821 atgagaatta ctataaagca tttatcaaaa attaagtttt taaaattttt agataataag
    38881 attgaatcca tagaaagttt tatagatgat tatagta
//
LOCUS       DBGJFF010000025        38668 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.025, whole genome shotgun sequence.
ACCESSION   DBGJFF010000025 DBGJFF010000000
VERSION     DBGJFF010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 38668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38668
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.025"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            <1..848
                     /locus_tag="ACOIAF_001324"
     CDS             <1..848
                     /locus_tag="ACOIAF_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5808 domain-containing protein"
                     /protein_id="HGZ2668340.1"
                     /translation="SYFIGIIGFLLYESILYIHTHKKAKKLKSEIYAKLGHIDIDSKL
                     IVDMDFINKKNKIIKKFKIIYLIPILFTLGMSIFIAFNYNQLPDSIATHWNINGSPDV
                     FIDKSFLNVFKLIGTDFCLMVLLYITSIGSIKSRIKIDTNRIEESRVENIKYLNKIGY
                     LFLILMIIMTTQFFTTLLSIKTKLSTAMNITTLLVIIYLIATYINSPNLKFNSSYSPE
                     EDEKYWIAGIMYNNPNDPSLMVNKRFGIGWTINFGNPLGKILYIAIALLLIFSLFSLI
                     KSLLL"
     gene            968..1444
                     /locus_tag="ACOIAF_001325"
     CDS             968..1444
                     /locus_tag="ACOIAF_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HGZ2668341.1"
                     /translation="MLSKAEIKYFNECASEILSSEKVQLMRTFPHHGNVSCLEHSLSV
                     AYYSYLLCKKLHLSVDIQSVIRGALLHDFFLYDWHYKGDRKGLHGFTHPREALKNATL
                     FFQINEKETDIILKHMWPLTVKPPRYKEAFIVCLLDKFCCLVETLKIHSLLSPYHV"
     gene            1830..2297
                     /locus_tag="ACOIAF_001326"
     CDS             1830..2297
                     /locus_tag="ACOIAF_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HGZ2668342.1"
                     /translation="MFKEMRLKKREMTKEDTVEVLKNGEFGTFSTISENGYPYGVAVN
                     YVYFNDSIYFHCARNGHKLDNISKNNKVSFLVVANESVIPDKFSTTYSSAIVFGKACT
                     VENEEKKNALVEIIKKYSKGFFEEGMKYIEKDMNLTTVVKIEIDHISGKASRL"
     gene            complement(2357..2929)
                     /locus_tag="ACOIAF_001327"
     CDS             complement(2357..2929)
                     /locus_tag="ACOIAF_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HGZ2668343.1"
                     /translation="MKVAVIFHSVCGSTYLLAREYKEALEEMNIEVGIFRVSDEVAKT
                     LPQYYLINSKEYKDEFESINVIKSGKEILDYDAIFMGSPTYYGNVSGPMKMFMDSFSD
                     IWVGAPLSGKIFGCFATAGSQHGGGELALQAMNIFAQHMGMTLLSVPCSVRGGYPAYG
                     ILHIAGDNSDIRPNDDDKIGIRDYLKRLNI"
     gene            complement(2992..4089)
                     /locus_tag="ACOIAF_001328"
     CDS             complement(2992..4089)
                     /locus_tag="ACOIAF_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HGZ2668344.1"
                     /translation="MLLKSLTRKIEKKYPLNLAEDWDNVGLIVGDFDMDVKKVLVSLE
                     ANEDVIDEAISKNIDLIVTHHPFIFGKINKINSGDLKGRLIQKLIKNDISLYSMHTNF
                     DIAFDGLNDYFMEIMEFGNSKVLDITKSENLYKLAVYVPHNYSDELRKVLSNSGAGHI
                     GNYSDCTFSIEGEGQFKPLEGSNPFLGSVNDIETVNEVKIETVVPQKLLGGVISSMLD
                     AHPYEEVAYDLYKLENKGEIFGLGRISKLDKSMTLESLSRKIKEKLNMKHIRVVGNLS
                     TDITKVAVVTGAGSEFVKKAKRQGAEVLITGDVKYHEAQDALDIGMCIVDCGHFDTED
                     IFKNVMKRFLDEFSEIEVIKSNVYLNPFSTI"
     gene            complement(4103..4792)
                     /locus_tag="ACOIAF_001329"
     CDS             complement(4103..4792)
                     /locus_tag="ACOIAF_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HGZ2668345.1"
                     /translation="MKLTDRLLKIASLVSDGKKIADIGTDHGYIPVYLLKEGKVPFAV
                     LADVNKGPLDNAHKEVIQNNLLDKVDLRLGSGIEILEIGEVEEVIIAGMGGILISELL
                     EAKKEVAHNVEKLILQPMQAQEELRYYLLNNGYEILEEVLVREDFRIYEIIVAKYTGK
                     NTIIEDEIYYEVGIKLLENKDSLFNDFIEKKIKTYSSIVNKLEGKNGEAIDKKRKESE
                     VAIKKLENLIK"
     gene            complement(4957..6123)
                     /gene="rpoD"
                     /locus_tag="ACOIAF_001330"
     CDS             complement(4957..6123)
                     /gene="rpoD"
                     /locus_tag="ACOIAF_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HGZ2668346.1"
                     /translation="MENKSNKKELKKVTAKTLIEKGKKQGSLTLAEIMEAFSETELDK
                     DQVENLYETLGNLGIEITETKNYKADIDFSVADDDLSIGHLDEDAEAISHDDSSAIEI
                     ETVDLSLPKGISIDDPVRMYLKEIGKIPLLKPHEEVEFARRMHEGDEIAKQRLVEANL
                     RLVVSIAKRYVGRGMLFLDLIQEGNLGLIKAVEKFDYTKGYKFSTYATWWIRQAITRA
                     IADQARTIRIPVHMVETINKLIRVSRQLLQELGRDPKPEEIAKEMEMTEDKVREIMKI
                     AQDPVSLETPIGEEEDSHLGDFIPDDDAPAPAEAAAYSLLKEQIEDVLGSLNDREQKV
                     LKLRFGLEDGRARTLEEVGKEFDVTRERIRQIEAKALRKLRHPSRSKKLRDYLD"
     gene            complement(6141..7931)
                     /gene="dnaG"
                     /locus_tag="ACOIAF_001331"
     CDS             complement(6141..7931)
                     /gene="dnaG"
                     /locus_tag="ACOIAF_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HGZ2668347.1"
                     /translation="MNDIKDIIEEIKARCDIASIISEYMNIKQSGANYKGLCPFHGEK
                     TPSFYINTSKQIYKCFGCGEGGDVINFVMKMENLDFMDAVKILANKCGIEINTNMNEE
                     TRIKIEKSKKFQDIHTEAARFYLSNLLGSKNLGYEYLRIRGLDDKIIKKFGLGFSLDS
                     WNSLMNTLISKGYKKQDLLECGLIAKNRDGTNCYDKFRNRVMFPIFDYRGNIIGFGGR
                     VLDDSLPKYLNSPDTLIFNKKQNLYGLNFARKNLESKTIVLVEGYMDLISLYQYGIKN
                     VVATLGTALTEQQGILIKRYADTAIISYDSDEAGIKATLRAIDILTKLGINVKVLDLK
                     DAKDPDEFVRKYGLSDYKKAMDVSTHYIKYKIDHLKKEFNIQKDEERVKFAKEASKII
                     KQLTSPVEIDFYTKYLSNQIDINVESIKREVYGKNYNKPYNNKNQKKIEEKVIEKVEV
                     RQDGKQLVEETLIKIMLEDKKIREIALLKVEESDFLLKESKEILNYMIKNQELDKITI
                     DKLKSLNISEEYLKELNSISLNSINLENTKEIEGIITNIRKNSLEEQINSLLREQQEL
                     ENNNDMKEVDGRVMEIALKIVEINKILKSL"
     gene            complement(8186..8971)
                     /gene="aroF"
                     /locus_tag="ACOIAF_001332"
     CDS             complement(8186..8971)
                     /gene="aroF"
                     /locus_tag="ACOIAF_001332"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HGZ2668348.1"
                     /translation="MKENLFNNNSKIEINTNNKKITVKKDKLIIAGPCAIESYEQLLE
                     TAKFVKSQGANILRGGAYKPRTSPNSFQGLKKEGLEILKAVKDEVGMAVITELMDVRD
                     MDELYSISDIIQIGSRNMQNFTLLSEVGKQNKPVMLKRGIASTITEWIGASEYIAIEG
                     NSNIIMCERGIRTYNDYTRNTLDLAAVPIIQKETGLPVVVDPSHATGVRYLVKPMSLA
                     SFACGADGIMVEVHPDPENALSDGAQSLCFNEFEDLMKSINNY"
     gene            complement(8952..9458)
                     /gene="folK"
                     /locus_tag="ACOIAF_001333"
     CDS             complement(8952..9458)
                     /gene="folK"
                     /locus_tag="ACOIAF_001333"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HGZ2668349.1"
                     /translation="MNKAYLGIGTNMGDRFDNLSRACELLKNSDSIYKVKESSLYETK
                     PWGYTEQADFLNMCVEIETEFEPYELLEYCQEIERELHRERIVHWGPRTIDVDILFFN
                     DVVSTDERLTIPHPRIQDRAFVLIPLMDLNEELIINEKTIKEHLNLLSAEEREEVKEL
                     VGYERKPI"
     gene            complement(9451..9816)
                     /gene="folB"
                     /locus_tag="ACOIAF_001334"
     CDS             complement(9451..9816)
                     /gene="folB"
                     /locus_tag="ACOIAF_001334"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HGZ2668350.1"
                     /translation="MDKILLSNLGFYGYHGVLKEENFLGQKFFVDMELYIDSREAGLS
                     DDINKSVSYAEVYNVVKGITENKQFNLLEALAENIAEEVLNKFILINGVMVRVRKPEA
                     PVNGIYDYFGVEIRRTRDE"
     gene            complement(9827..10618)
                     /gene="folP"
                     /locus_tag="ACOIAF_001335"
     CDS             complement(9827..10618)
                     /gene="folP"
                     /locus_tag="ACOIAF_001335"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HGZ2668351.1"
                     /translation="MFDYGKRTYIMGILNVTPDSFSDGGDFNNLDIAIQHAKDMVDQG
                     ADIIDLGGESTRPGHSYVDSDEELRRVIPVIKKLKQELDIPISIDTYKADVAEEALKL
                     GVTMVNDVWGLRKDKNMASVIGKYDAEVCIMHNQDGTNYDKDIMESIKDFFKVSIEMA
                     MSCGVKKEKIVLDPGVGFGKDFEQNIEVLRRLNELKDLGYPILLGTSRKSVIGKVLPV
                     EPKKRLEGTIATTVLGIRDGVDIVRVHDVYENLMAARMTDAIYRK"
     gene            complement(10682..11254)
                     /gene="folE"
                     /locus_tag="ACOIAF_001336"
     CDS             complement(10682..11254)
                     /gene="folE"
                     /locus_tag="ACOIAF_001336"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007287029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HGZ2668352.1"
                     /translation="MNKVDKEKIQHAVREILEAIGEDPDREGLIETPNRVARMYEEIF
                     SGLSEEPRDHLKVLFADEKHEELVLVKDIPFYSCCEHHLVPFFGKAHIAYLPKGGRLT
                     GLSKLARVIDTLAKRPQLQERITKNAADIIMEELQPYGVLVVVEAEHMCMTMRGVKKP
                     GSKTVTSAVRGIFEKDIASRAEAMSLITMK"
     gene            complement(11280..11981)
                     /locus_tag="ACOIAF_001337"
     CDS             complement(11280..11981)
                     /locus_tag="ACOIAF_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668353.1"
                     /translation="MIGCIYEVYNEEITKIIENQNTLAVYLVGSSKDVDFTLYDVEIN
                     DIDVFVFVKEGEKQERILFKKKGIEFDVNYFSKDGVKKLLSNREYFFVKEMKDAKVLF
                     DRENISHIIKDISRNIYLEGPSKMSLEEKSFIKQDIGAKISNLKNKEKFDVFEYHFLT
                     NLYLKDIIIGYFNINDKWVPKDKKLLKVLKKENNELFELVSKVSENYDYQRLLDVYNY
                     IFKEIETKEVIKLIF"
     gene            complement(12015..12758)
                     /locus_tag="ACOIAF_001338"
     CDS             complement(12015..12758)
                     /locus_tag="ACOIAF_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HGZ2668354.1"
                     /translation="MKNNVGFDSSLSKFGIGLFETIRVKNGIAVDLDIHIERMLSSIN
                     CLDLDINYKKDFLINEIVTYIKKENVINKALRITVFDEGYNISIRDIPYNQETYEKGF
                     KLAISPIVRGNSLIHRHKTTNYFENIYTKNIANKTGYNDGIFLNSEGVILECSMSNIF
                     FIKGERVYTPSDRLPILNGIIKKRIIEICDELHIELIENEINISEISSFDFVFVTNSL
                     MGAIKVTEIDKIKFNKENVVFDKIVKLLE"
     gene            complement(12748..14097)
                     /gene="pabB"
                     /locus_tag="ACOIAF_001339"
     CDS             complement(12748..14097)
                     /gene="pabB"
                     /locus_tag="ACOIAF_001339"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HGZ2668355.1"
                     /translation="MIREINTKLNSFEIFTIFRNEHDSFILDSAMDKEKLGRYSFISS
                     QPFKVLKYKDTDENPLEVLKEELHKYRVVNDTNLPFVGGAVGYLSYDLGNYIENLPRT
                     AVDDIEMPDMYFGFYNHVIVIDHLVQKTYIATPNIDIELEEKIIDDIEQRILKEEKKG
                     IDSICYEEKEVTPIRLKSNFTKEEFKNAVQSVREYIRQGDIYQANLTQRFSGETELTS
                     FELYRDLRRFSPAPFGAFLNFEDAHILSNSPERFIRCVNKRIETRPIKGTRPRGKDKE
                     EDLRLQQELRNSEKDRAELLMIVDLERNDIGRISKTGSVKVPELFVIEPYANVNHLVS
                     TVVGELKDDKDATDVIKATFPGGSITGAPKIRAMEIIDELEPTQRNVYTGSIGYIGFN
                     GDMDFNIAIRTIIKNDKKVYFQVGGGMTWDSDPDEEYQETLDKAKSIMKALRGYYEE"
     gene            complement(14099..14683)
                     /locus_tag="ACOIAF_001340"
     CDS             complement(14099..14683)
                     /locus_tag="ACOIAF_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HGZ2668356.1"
                     /translation="MILMIDNYDSFVYNLVQYIEELGETVVVKRNNEIKISDIEELNP
                     EVIVLSPGPCSPKEAGICIDIVEHFKGKKPILGICLGHQTIGHVFGGDIIKAQQPVHG
                     KVYSINHTNKGVFRGLKNPLNVTRYHSLIVDSNTVPKELEITAITDKGEIMGIRHKKY
                     LIEGVQFHPEAILSEYGHEMLKNFITEARERVHM"
     gene            complement(14707..15426)
                     /locus_tag="ACOIAF_001341"
     CDS             complement(14707..15426)
                     /locus_tag="ACOIAF_001341"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HGZ2668357.1"
                     /translation="MTSWKKKTVYKCLIAVVLFCGIVLISNFSKVSALMMDTNGNVLI
                     KHGSREKKLIAITFDDGPHPKETSQVLDVLKKYNVKATFFIAGKHAKWYKEPLVRASK
                     EGHEIGNHTFNHPDISNLSSSQIEEEIVKCEDILKEVTGKKPTLFRPPFGSYREKELI
                     EIAKKHDYKVVLWTGVDVKDWKNPGANSIADKIINKVQNGDIILLHDYATNDTVEALD
                     MFIPKMIEKGFKFVTVSELIK"
     gene            complement(15454..16518)
                     /locus_tag="ACOIAF_001342"
     CDS             complement(15454..16518)
                     /locus_tag="ACOIAF_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HGZ2668358.1"
                     /translation="MKRLIALVLVVLIVLFYSPELLKEGYDLKEKFFQSEIWNEFLDK
                     ININDNSKKDKKEKSQNLQSNNTEGENLGESDIKNFEKISLGDNLSYVLSSIGKPGRI
                     DISEYGFDWYVYNQYGKEFAMVGLENDEVVALYSNSINSCENQDIKLNQDRQTVRTKI
                     TPLKYKRKGNTRYIINSENQYDIISKEGKYITIFYDIHEENRVCSYLIIDKSTEDEFK
                     NLYPDDSEELKKCFELEVIDLVNSVRNQRGLNSLRYSEQATLSSRKHSEDMRDNNFFD
                     HVNKKNETPFDRMKREGIVYTSAGENIAAGQINAIYAHEAWMNSEGHRKNILGNYNNI
                     GVGVIFGGSYKTYYTQNFYK"
     gene            complement(16662..17471)
                     /locus_tag="ACOIAF_001343"
     CDS             complement(16662..17471)
                     /locus_tag="ACOIAF_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MORN repeat-containing protein"
                     /protein_id="HGZ2668359.1"
                     /translation="MNLSKEYVNLYDNYLKYINEVNKEIRSIKNMKKRVLKRGDFAPE
                     NEVLELEGKTIRDIDDVDTKKPKNKIYKFSNGDVYVGKFLEGKMEGKGSYTFFVDEGT
                     VMEYIGEFKEDKKNGKGNFIFSNGNEYIGYFEEDMMSGIGNMLYSSKDEYIGTWKNGK
                     KDGKGIYKWSDGCIYAGEFKGGKMEGYGICYNSKGEVLYEGEWKNNLIHGKGTYIWEK
                     GKKYIGEFMHGKKHGQGTFYLNNELVYEGTWKFDKPSIFDRTLDEIFSLRL"
     gene            complement(17713..18579)
                     /locus_tag="ACOIAF_001344"
     CDS             complement(17713..18579)
                     /locus_tag="ACOIAF_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HGZ2668360.1"
                     /translation="MSEVKNTLQKHPVLRYSTMLFGMLMTSIGINGFLRPAHLLSGGA
                     TGIATSINYLTNINVGLLTFLINIPIFILGFIYLEKEFCISSLVNMIVFSLLLGATQE
                     ISNIIPIHDILLQSVYGGILSGLGVGVVFRTRSSQGGTDIIAAILKIKKNIEMKDTAL
                     AINGLIVLTGSFLFGLDLALYTLIGLFLNAYSMSFIKDAMNYQKSVMVMSNEVDLIAE
                     DIMKSLVRGVTFLDAEGAYTHQKKKIIYTIVSSNEIPKIKDIALKYDKKAFISVNDVT
                     EVKGRGFKAKDL"
     gene            complement(19099..19788)
                     /locus_tag="ACOIAF_001345"
     CDS             complement(19099..19788)
                     /locus_tag="ACOIAF_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HGZ2668361.1"
                     /translation="MYNVLQTPVFGIIVTIVFFNLGRYIQRKTSNPICNPLLIAIVGI
                     ILFLSISKIPYENYKIGADSLNFFLGPVTVVLAVPLYKQFELFKKHMFEIIVGIGCGI
                     VISFVSVLIIGKIANSDVSIINSLIPKSITTPMGISLANSLNGVESITVVAIIFTGIF
                     GGMVASTVFKLGNISHPVAKGIALGTSAHALGTTKAFELGEIEGAMSGVSIGVSGTIT
                     VILIPIIMNFI"
     gene            complement(19781..20152)
                     /locus_tag="ACOIAF_001346"
     CDS             complement(19781..20152)
                     /locus_tag="ACOIAF_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HGZ2668362.1"
                     /translation="MKVFNQLVIILSIWVIGEYISSFIQGIILIPGSIVGMLLLFLLL
                     QFKVLDLSSIENVSGFFLDNMAIFFIPAGVSLIKSLDLIRENVLVLLLVICLSTLIVM
                     YTTGIIVEKMIKKKEEGQKNV"
     gene            complement(20334..21743)
                     /locus_tag="ACOIAF_001347"
     CDS             complement(20334..21743)
                     /locus_tag="ACOIAF_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HGZ2668363.1"
                     /translation="MEVSIILVKDIKQDIESMPRKILDISLIEDNKSDLTFFNIEEED
                     GDETLKALKQSYVTTNYKEETLLNLQGALNSKSDTTSKELKLESYKRYNNALDLAYKN
                     YITSAINMVNKALEINPKDIDILNLRGLLKLLKCDFAKSFESFYTALCYENNHISRKY
                     VNLISSEDFKTFLGRYNHSIRFINEELNYESIHILENIIEEEPELIEPYVILSLLYDK
                     LGNVKKREGYLDKLKELDKDNPVFEKNEEEKEEEKEDTSKNEEIKKVKRKKSILPYII
                     VGCVLIGMGIYLIQSKKRIENLNNQISSKEEKLSETDKKLGETSKKLEKTNKELNEVK
                     NKEPEREITVADEEDLYYQALNLKKNKEYEKAIDNFKSIVSSGKTKKYISESIYQLAI
                     TNKLLGNKDEAIKYYKKYINTYTKNDQYYDDSYYELGMLYYDNGDLKNAQQTFYSLRS
                     EVPDSMYNNSKIKEILSEK"
     gene            complement(21874..22467)
                     /locus_tag="ACOIAF_001348"
     CDS             complement(21874..22467)
                     /locus_tag="ACOIAF_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGZ2668364.1"
                     /translation="MNLNNITNYVQSICENISNVLDVDVTLVTKDLTRIAGTGIFRGK
                     IGETISEKSVYSLVLKDGKSYVINKEIEENCNKCANRENCKELADICTPVKMGEHILG
                     ILGIAAFNEEQKEKILSKDKELTAFVRSMSDLISFKLDELYKQEVKTIDDLEREAIEN
                     AIKKYGSNTEGMKKVAEALNIGIATLYRKVKKFNIKS"
     gene            complement(22532..24001)
                     /locus_tag="ACOIAF_001349"
     CDS             complement(22532..24001)
                     /locus_tag="ACOIAF_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HGZ2668365.1"
                     /translation="MSNVNKKLVIAIGAVALVVIIFIGIMCMQFKGEKIAKNTYVNGV
                     NIGKLTKSQAKQELAKKYKLENVDFNYNDKSWKVKSKDLNLSTDLDKTVENAYNLNRK
                     SAFFGNLSKTISANFGKKSNLVVVINYDKNKLKAEMEKIAKEIDVDVKDATLDISGEK
                     VKVIPDSDGLKMDISKSMENFDNQTKKGNYKNELVVKATPAKVKKEQLANIDTNLGTY
                     STTFKTSQINRSINIKLATDNISNVLLMPGETFSFNKHTGKRSKENGYKSAPVIMEGE
                     MEEDYGGGVCQVSSTLYNSVLYAGLEIVNVKNHTIPSSYVPKGRDATVADSGIDFLFK
                     NNLKHPVYIKNYVSGNQIVCNIYGSAEDKQNITISTKLDGVSQTTMKRVNDPTMPKGK
                     EKVDKSGRNAYSVSTYRTFNDANGKKIKTEKIANSYYPKKEGIILVGTMEPKPEEKPN
                     TDENKNNQNTNNQNPNNQQKPETPPTDNKPNETQPQPQA"
     gene            complement(24112..25503)
                     /locus_tag="ACOIAF_001350"
     CDS             complement(24112..25503)
                     /locus_tag="ACOIAF_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HGZ2668366.1"
                     /translation="MNFRDLFEKAVDFIDEKRKSIVAISLSTLGVIALIIVFFLSSNE
                     FSVGNEANELLKIIEKRQYSIAVDYYTSIEKKFSDSKMERFNKSVSKKINKLLLNSGD
                     KYLDGDISQESFIGLINTVKELNKISIDVDDLLAQADRVREMYKEENTTYDIAINYIS
                     TVSILNGMGSNLDVYKQNIETIKESRDVYDSAVKDQKAYKYYEAIEKYNKVLKEDEKY
                     YSMAQNGKEQCIEEMYDYYISRAEEANNSGDYERALQYIEYLKDDYSDDEKVQSLEKK
                     YKKNLSLYTLTSDDIINVIAKKSGKDKANLSINSLPQMIKNNKYYYAEVYEYDKLINE
                     VLVSAKTKDLYSYKDGKKDYKVDYGDGYFRILEDGSYQFGITKDKAKFVLTNTLDEKE
                     NKYKKIEILDIEKADRYVKSKKSLEELFGKYKNIYYYAVVNKGLFRGKEVYAINIYNE
                     KIYAISENGLNEY"
     gene            complement(25669..25959)
                     /locus_tag="ACOIAF_001351"
     CDS             complement(25669..25959)
                     /locus_tag="ACOIAF_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668367.1"
                     /translation="MSGLKKILRILVISMMICFAMTFALVLINSVMGVHIGLDYYFLM
                     GSVLTVCLTIGIVLCNARKIFDSKAIHKTRTKTVSNNKKQKSHSRDIKRKIS"
     gene            26183..28312
                     /locus_tag="ACOIAF_001352"
     CDS             26183..28312
                     /locus_tag="ACOIAF_001352"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HGZ2668368.1"
                     /translation="MPNLPSLGSKAPDFKANTTNGPIRLSDYKGNWIVLFSHPGDFTP
                     VCTTEFLCFAKYYDEFKKRNTELIGLSVDSNSSHLAWMYNISLLTGVEIPFPIIEDRD
                     MRIAKLYGMISKPMSDTSTVRSVFIIDNNQILRTILYYPLTTGRNIPEILRIVDALQT
                     SDRDNIVTPANWFPGMPVILPYPKNYKELKNRVNSCNKKYSCMDWYLCFVPDNYNDEE
                     VSKKIDNTCSWKKEHTKNIENECNCEHEHHDYLNKALDCKQEHKTDIKDDCNHEKKHT
                     KNTNKVHNSKQDKFKDKSCDEMNFNYDKDESCDKINSSYNKEDSSYEDFYKHNYKNYD
                     YTSEKNTKKIAMKTLKDSKKLVRPQITDPYNPIVENANCPDINPIVAEYVLGNPTNVD
                     AQLLDAVIFAFAEIDQSGNLFIPYPRFLNQLLALKGEKPSLKVIVAIGGWGAEGFSDA
                     ALTPTSRYNFARQVNQMINEYALDGIDIDWEYPGSSASGITSRPQDRENFTLLLTAIR
                     DVIGDDKWLSVAGTGDRGYINSSAEIDKIAPIIDYFNLMSYDFTAGETGPNGRKHQAN
                     LFDSDLSLPGYSVDAMVRNLENAGMPSEKILLGIPFYGRLGATITRTYDELRRDYINK
                     NGYEYRFDNTAQVPYLVKDGDFAMSYDDALSIFLKTQYVLRNCLGGVFSWTSTYDQAN
                     ILARTMSIGINDPEVLKEELEGIYGQF"
     gene            complement(28539..29138)
                     /locus_tag="ACOIAF_001353"
     CDS             complement(28539..29138)
                     /locus_tag="ACOIAF_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viroplasmin family protein"
                     /protein_id="HGZ2668369.1"
                     /translation="MAKQKFYAVKKGKNIGVYNTWDECKKQVNGFSGAEYKSFSTFQE
                     AKEYIDGSEKLSFQEDKEFIEAYVDGSYEHSVKMYGSGVVILKNNEVIKTYSEKGKEK
                     TLVSMRNVAGEIEASKIAMQYCIDNNVQNLILYFDYEGIEKWCTGVWKTNKEGTIAYK
                     NFYDSIKNKLNVKFTKVKAHSGNKYNEEADKLAKKAIGV"
     gene            complement(29156..32275)
                     /locus_tag="ACOIAF_001354"
     CDS             complement(29156..32275)
                     /locus_tag="ACOIAF_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HGZ2668370.1"
                     /translation="MDNIISVLNDVVFLDIEVSGLDCLNSEILEVGAVKVKDWKIYTY
                     ESLIKNKFEVPVEVFSVCKNLDKNDLEIANEIELVEDRLVNFVEDSFIICHDLSLKKK
                     FFEYHMPKLKNKFIDLIELAVILEPYHKDYSLEYLKNTLTNCNSKVENRALSDAIDII
                     NIVNCLLVKFNNYEKTTLEPLSFKINSYLKKFNLPTWEWSKFLEEANYDLSNNINIKK
                     EYNIFDSKEEKKKERETLKILNEEEKNYEELLKYKTIWENKEGFTYEYRPGQYELTKT
                     IRELFRNSEDEEKIACIEAPTGIGKSVGYLLPAILEARINKKRLLISTDTKELQIQLI
                     NKDIPNVLDSLGLNGKVSYGYIKGKNNYICIDRLEAYIDDYESQNPTKGEILSLIFLK
                     RLVEGGKYGDIEEINYLVFDNFKEISTHLRNVSCDPNMCRPKKCKKDCLYKNRIEELK
                     EEHITVVNHSLLAKWPYKDEKPLENIIVDEAHNLTEKGYDFFSSIINSKSLRYLLQEI
                     YPYEFIQNSSFIYKKYSRNMRKIKAFDKFYNVLKIGREDKQKIARSINLIIEEIDSIL
                     NFGNCNEYNNVSNYNLRWELNLQIDEIVGKLKKDGIDTEISYRAYSEKIKLSCEKIIK
                     NLVSIIIIIYRNIDDDSIDKEADIYKFGKAKTRDLEDIKIIFEIFLEYDEKDDYARIV
                     EIDKNYNVFEFRVVPLKIADLFEENILSQLEKGIFLSATLSLSESMSYFKNTLGIDRV
                     KNVEKIIEPIFDYKNRVSVVGFSDICEYRNSEFPNEMSKIISNISKITEGHTLALFNS
                     KDRQEKTYEILKKYLHSFNLEIYADKKGIRHLNDLNRKCVVLGSKGCFEGVDIPGDGL
                     VCVTLDKLPNLNPKDPLYFTIMKKYGIDYYTINYPQMTIKVKQAMGRILRSKYDYGCF
                     VIFDVGTNISVLKRLEKDLHDCKISKVNSNEFYTYIRRHLNKSRSLILKSVIFDTIKA
                     LNVDAKMDNNDVDKDIIKKDINENIRQRAVKGEVYHIDIIKKDMKVKYFDRNYLINLD
                     IFMREEDKN"
     gene            32496..33320
                     /gene="pdaA"
                     /locus_tag="ACOIAF_001355"
     CDS             32496..33320
                     /gene="pdaA"
                     /locus_tag="ACOIAF_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HGZ2668371.1"
                     /translation="MKKDSLKKYIMIGAFALILFGIASINFKSLDKTKTQISSPTLDT
                     HEYDWYFNPREDGKQPSPIKEADFFKKYGAYYVGNPNEKVIYLSFDAGYESGNTPKLL
                     DTLKKHNAKAQFFVVESYIKSNPELIKRMEKEGHLVCNHSKSHPSMAGITDFEKFKEE
                     ITSVEKAYKDVTGKEMPKYFRPPMGKFSEQSLKYTQDLGYKSIFWSFAYVDWYEKKQP
                     THEFAKNKIYSRTHPGAIVLLHPNSSTNTEILDEVLTHWEKEGYKLKTLDYLNNKK"
     gene            complement(33417..34202)
                     /locus_tag="ACOIAF_001356"
     CDS             complement(33417..34202)
                     /locus_tag="ACOIAF_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGZ2668372.1"
                     /translation="MIVKIDNTLEKEFWQYVSHEESLNLFIIGYVENYGFSSQYQDIW
                     SQVEDGNITSIILKNKSTLIIYSFKNNFNIGEMKNHIKDLDVESISGKKCVIDRLISK
                     YKDFYEKLDNKFCVLKEIKEIDFSNMKEYKIENAQEKDIDEIGKLLNRSDYKVSKNYI
                     EERKVHLKEGNVRAYFIRNDDTMISTVSTGMETSFLAMVVSVSTDKRYRGKGLASYMV
                     YNLSKELLLEGKVPCLFYNNDVAGKIYHNIGYKEINEWTILFK"
     gene            complement(34302..34436)
                     /locus_tag="ACOIAF_001357"
     CDS             complement(34302..34436)
                     /locus_tag="ACOIAF_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668373.1"
                     /translation="MNISRKAMKIIELAQKIANKRGISVEEAWSEAVTEYKNKYEHIA
                     "
     gene            34667..35032
                     /locus_tag="ACOIAF_001358"
     CDS             34667..35032
                     /locus_tag="ACOIAF_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HGZ2668374.1"
                     /translation="MKIRAEYTCPLEIVHDIIKGKWKTILIFELRQSNKTFSELQHNI
                     YGINQKMLIQQLKELRDFGIIDKISSAGYPLHVEYFLTNRGKKMLKAVEIMQEIGIEY
                     MLEHGQQEILDNKGICYNK"
     gene            35110..35664
                     /locus_tag="ACOIAF_001359"
     CDS             35110..35664
                     /locus_tag="ACOIAF_001359"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HGZ2668375.1"
                     /translation="MSIALILIDIQNDYFKNGKCELFQSEETAENAKKILLFFRKNNL
                     PVIHIQHISTDKTASFFLPNTHGSEINKIVFPLDNEEIIIKHTPDSFFETDLQSILEK
                     KNITNLVICGMMSHMCIDTSVRTAKKLRYDITLISDACTTKNLSWNNIEINANTVHQV
                     FMASLQNSFADVKNTDKFLSNYHK"
     gene            35884..36558
                     /locus_tag="ACOIAF_001360"
     CDS             35884..36558
                     /locus_tag="ACOIAF_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HGZ2668376.1"
                     /translation="MNHHILIQFEYIARIITAGFCGALIGYERKNRLKEAGIRTHFIV
                     ALASALIIIVSKYGFNDIVGTPGIGLDPARVAAQVVSGIGFLGAGLIFIRNQSVSGLT
                     TAAGIWATAGTGLAIGSGLYLVGIVSSIIIVIVQITLHRDRKWMKLPTAEQVSLKISN
                     STDSIEYIEGKFKKSNIEIINMNIKRLDGDWLEVVIYTKLPKGYKKTNLLKLFSDNPL
                     IETLEI"
     regulatory      36913..37009
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            37063..37239
                     /locus_tag="ACOIAF_001361"
     CDS             37063..37239
                     /locus_tag="ACOIAF_001361"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668377.1"
                     /translation="MFRDEMDKCTHMLTAYISSLYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     gene            complement(37426..37665)
                     /locus_tag="ACOIAF_001362"
     CDS             complement(37426..37665)
                     /locus_tag="ACOIAF_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668378.1"
                     /translation="MKCSNCGSVNIEKGILTTGGIYAETIGLKYRGDRKLDKVINKTF
                     PVQSTIYSDLCLDCGEIVRTYIKDNIDRNWCKDKK"
     gene            complement(37810..38619)
                     /locus_tag="ACOIAF_001363"
     CDS             complement(37810..38619)
                     /locus_tag="ACOIAF_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HGZ2668379.1"
                     /translation="MKDYYKIGEISKIYGIGRDSLMYYEEIGILRPVRDINGYRMYNI
                     SDIWKLNLIKEFRSLNFPMKKIKEYLDDRSIESTKNILNEELDLIDKKIAEFISHKEN
                     IIKRLSSIESVIQNTKIDEIEVVYIEKRKALELNADIKRDEDFDFLIQKLQKEYEDRF
                     NILGNNNIGSAFSTEAINKGIFNEFKSVFCFLESNEKVYNIVFDEGYYVTLNYTGSNS
                     NNKMYMEKVFKFIEENNYKIIADPIEIYKIDIHETGIVEEFVTEIQVPITK"
BASE COUNT        12507 a         6561 c         3974 g        15626 t
ORIGIN      
        1 tttcctattt tattggtatt ataggttttt tattatatga aagtatacta tatattcata
       61 cacataaaaa agctaagaag cttaaatcag aaatttatgc taaattaggt catatagata
      121 tagattcaaa attaatagta gatatggatt ttataaataa gaaaaataaa ataataaaaa
      181 aatttaaaat aatctattta ataccgatac tatttacttt gggaatgagt atttttatag
      241 catttaatta taatcaattg ccagactcaa ttgccactca ttggaacata aatgggtctc
      301 ctgatgtttt tatagataaa tcatttctta atgtatttaa attaatcggt actgactttt
      361 gtttaatggt tttattatat ataacttcca ttggatccat aaagtcaagg attaagatag
      421 atacaaatag aattgaagaa agtagagttg aaaacattaa atatttaaat aaaattggtt
      481 atttgttttt aatattgatg attataatga caacacagtt ttttactact cttttatcaa
      541 taaaaacaaa actatcaact gctatgaata ttacaacgct tttagttata atttatttaa
      601 tagcaacata tattaattcc ccaaatttaa aattcaactc atcttattct ccagaagaag
      661 atgagaagta ttggatagca ggcatcatgt ataataatcc aaatgaccca tcattaatgg
      721 ttaataaaag gtttggtatt ggatggacta taaattttgg caatcctctt ggaaaaatac
      781 tatatattgc tatagcttta cttttaattt tttcattgtt tagcctaata aaatctctct
      841 tactttaaaa taccataaaa ggtatcttac ttgtattatt gggtattata ttttgtagga
      901 taagaaatat ttcatattat cgttgaagta tagtatttat atattactaa gaatggagat
      961 tttcataatg ttgtcaaaag ctgaaatcaa atattttaat gaatgtgctt ctgagatact
     1021 ttcttctgaa aaagttcaac ttatgagaac atttcctcat catggcaatg tttcatgtct
     1081 tgaacatagt ttgtcagtag catattatag ttatctatta tgtaaaaaat tgcacttaag
     1141 tgttgatatt caaagtgtta ttagaggagc tttactacac gacttttttc tttatgattg
     1201 gcattacaaa ggtgatagaa aaggattaca tggatttact caccctagag aagcattaaa
     1261 aaatgcaact ttattttttc aaataaatga aaaagaaacg gacataattt taaaacacat
     1321 gtggccactt actgttaagc cacctagata caaagaagca tttattgtat gtttgttgga
     1381 taaattttgc tgtttagttg agactttaaa gatacattct ttattatctc cttaccatgt
     1441 ataggacaat tcccacaaca cttttataac ttaattttga agaggtatta agtattatat
     1501 tcaacttgat acctctttac tatttattat tttatataat aatattttct ttataatatc
     1561 gttataaata acaacttcta ccctcgataa cttccccttc ttatagacat gcttactatc
     1621 ttaaataaca agaaaccagc tattaaattt attagtattg tttatactac tctaacaata
     1681 atatcataaa taaacctcca aatagcttct ctttcactac cttttatctt aaactatatt
     1741 tttttcttaa aatttgtaaa aaacatagca taatctttat aaatgttcta aaatttaatt
     1801 taagcatcaa aattagaaag gatgatatta tgtttaaaga aatgagattg aaaaaaagag
     1861 aaatgacaaa agaagatact gttgaagtat taaaaaatgg tgaatttggt actttttcca
     1921 ctatatctga aaatggttat ccttatggag ttgctgtaaa ttatgtatac tttaatgatt
     1981 ctatttactt tcattgtgca agaaatgggc ataagttaga taatatatcg aaaaacaaca
     2041 aagtttcttt tttagtagtt gctaatgaaa gtgttattcc agataaattt agtactactt
     2101 attctagtgc tatagttttt ggaaaagctt gcactgtaga aaatgaagaa aagaaaaatg
     2161 ctcttgtaga aataataaaa aaatattcta aaggattctt tgaagaaggc atgaagtaca
     2221 tagagaagga tatgaactta acaactgttg ttaaaataga aattgaccat atttctggta
     2281 aagcttcacg tttataatta agtaaaaata cctaaaagta tatacagtcg cttttaggta
     2341 tttttatatg atattcttat atgttaagtc tttttagata atctcttata cctatcttgt
     2401 catcatcatt tggtcttata tcagaattat ctcctgctat atgaagtatt ccatacgctg
     2461 gataacctcc tcttactgaa catggaactg acaaaagagt cattcccata tgttgggcaa
     2521 atatattcat agcttgcaaa gctaattctc ctcctccatg ttgtgaacct gctgtagcaa
     2581 aacaaccaaa tattttacca ctcaaaggag cacctaccca aatatcagaa aaactatcca
     2641 taaacatctt cattggtcca gatacatttc cataatatgt tggtgaaccc ataaatatag
     2701 catcataatc taatatttct tttccagatt taattacatt tatactttca aattcgtctt
     2761 tgtattcttt ggaatttatc aaataatatt gaggaagtgt ttttgcaact tcgtcactta
     2821 ctctaaatat acctacctcg atattcattt cttcaagcgc ttctttatat tctctagcta
     2881 ataaatatgt actcccacaa acactatgaa aaattactgc tactttcatc attatccccc
     2941 taattaaact caaatttatt ttttatatag tatttttatt atcaaacact gctaaatagt
     3001 actaaatgga ttcaaatata cattactttt tattacttca atttcactaa attcatctaa
     3061 aaatctcttc ataacatttt tgaatatatc ttctgtatca aaatgaccac aatccactat
     3121 acacattcca atatcaagtg catcttgtgc ctcatgatac tttacatcac ccgtaattag
     3181 tacttctgca ccttgcctct tagctttttt tacaaattct gaaccagctc ctgttacaac
     3241 agctactttc gttatgtctg tactaaggtt tccaactact cttatgtgtt tcatgtttaa
     3301 tttctctttt attttccttg aaagactttc taatgtcata cttttatcaa gtttacttat
     3361 tctacctagt ccaaatatct cacctttatt ttctaacttg tacaaatcat atgcaacttc
     3421 ttcataagga tgtgcatcta acatagaact tattacccca cctaaaagct tctgtggtac
     3481 tacagtttca attttaactt catttacagt ttctatatca tttacacttc caagaaacgg
     3541 attagagcct tctaatggct taaattgtcc ttcaccttct attgaaaacg tacaatcact
     3601 ataattccct atatgacctg ctcctgaatt acttaaaacc tttctaagtt catctgaata
     3661 gttatgtgga acgtatactg ctaatttata caaattttcg cttttagtta tatctaaaac
     3721 ctttgaatta ccaaattcca taatttccat aaaatagtca tttagaccat caaatgctat
     3781 atcaaaattc gtatgcatag aataaagtga tatatcattt ttaataagtt tttggataag
     3841 tctgcctttt aaatctccac tattaatttt attaattttc ccaaatataa aaggatgatg
     3901 tgtcacaatt aaatctatat ttttggatat agcttcatca ataacatctt cattagcttc
     3961 taaagatacc aatacctttt ttacatccat atcaaaatct ccaactataa gacctacatt
     4021 atcccaatcc tctgctaagt ttagaggata ctttttttca atttttctag ttagactctt
     4081 taatagcata tatacctcct atttatttta ttaaattttc aagttttttt attgcaactt
     4141 cgctttcctt tctcttctta tctatagctt ccccattttt cccttctaat ttattaacta
     4201 tagaactata tgtctttatc tttttttcta taaaatcatt aaaaagagaa tctttgtttt
     4261 ctaaaagctt tattccaacc tcataatata tctcatcttc tattatagta tttttacctg
     4321 tatatttggc aacaattatc tcatatattc taaaatcttc tctcactaaa acttcttcta
     4381 aaatttcata tccgttgttt aaaaggtaat atctaagttc ttcttgtgct tgcattggct
     4441 gtaatattaa tttttctaca ttatgtgcta cttctttttt agcttcgaga agctcactta
     4501 tcaagattcc acccatacca gcaattatca cttcttctac ttcccctatt tcaagtatct
     4561 ctataccaga acccaatcgt aaatcaactt tatctaaaag attgttctgt atgacttctt
     4621 tatgtgcatt gtcaagaggt cctttattaa catccgctag cactgcaaat ggtacttttc
     4681 cctcttttaa caaatatact ggtatgtatc cgtggtccgt tcctatatca gctatttttt
     4741 taccatctga cactaatgat gctattttca ataatctatc tgttaatttc aaatataatt
     4801 ctcctttcaa ttccctttat ttataggatt taatccaagt tatagatatt agactgaaat
     4861 aactacaaaa ataaaattaa atctttacta caaaatagcc atgagatatc acttatctca
     4921 tggcatttat cacaaaaaca tcaatgatgt atctttttag tctaaataat ctctaagttt
     4981 ttttgatcta cttggatgtc ttagttttct tagtgctttt gcttctatct gtcttattct
     5041 ctctctagtt acatcaaact cttttccaac ttcctcaaga gttctggctc taccatcctc
     5101 aagaccaaat ctaagcttta atactttttg ttctctatca tttaatgaac caagtacatc
     5161 ttctatttgt tcttttaata aagaatatgc tgctgcctct gctggggctg gagcatcatc
     5221 atctggaata aagtcaccta aatggctatc ttcttcttct cctattggtg tttctaaaga
     5281 cacagggtct tgagctattt tcattatttc tcttacttta tcttctgtca tttccatttc
     5341 ttttgcaatt tcttctggtt ttggatctct tccaagttct tgaagtagtt gtcttgatac
     5401 tcttattagc ttatttatag tctctaccat atgaactggt attcttatag ttctagcttg
     5461 gtctgcaata gcacgtgtta tagcttgtct tatccaccat gttgcataag tactaaactt
     5521 atatcctttt gtatagtcaa atttttcgac tgcttttata agacctaaat ttccctcttg
     5581 tattaaatcc aagaaaagca tacctcttcc tacatatctt tttgctatac ttacaactag
     5641 tcttaagttt gcttcaacta acctttgttt tgctatctca tcaccttcgt gcatccttct
     5701 agcaaattct acttcttcat gtggtttaag tagaggaatt tttcctattt cttttaagta
     5761 cattcttaca gggtcatcta tacttatccc ttttggtaaa gataaatcca cagtttctat
     5821 ttctattgca gaagagtcat catgtgaaat tgcctctgca tcttcatcta agtggcctat
     5881 acttaaatcg tcatcagcaa ccgaaaaatc tatatcagct ttatagtttt ttgtttctgt
     5941 tatttctatt cccaaatttc ctaaagtctc ataaagattt tctacttgat ccttatcaag
     6001 ttcagtctcc gaaaaagctt ccattatctc tgcaagtgtc agagaacctt gcttttttcc
     6061 tttttctatt aatgtcttag cagtaacctt ttttaactct tttttattcg atttattttc
     6121 cacactcaaa cctcctttct ctacaagctc tttaaaattt tatttatctc aactattttt
     6181 aaagcaattt ccataactct accatctacc tctttcatat cattattatt ttctaattct
     6241 tgttgttctc tcaacaagct attaatttgc tcttctaatg agttttttct gatatttgtt
     6301 attattcctt ctatttcttt tgtgttctct aagtttatac tattcaaaga aattgaattt
     6361 aattctttta agtattcttc ggatatgttt aaacttttca atttgtcaat agttattttg
     6421 tccaattctt gatttttaat catgtaattt aaaatttctt tactttcttt caataaaaaa
     6481 tcactttctt ctactttaag taatgctatt tctctaattt ttttgtcttc taacattatt
     6541 tttattagag tttcttcaac taattgtttt ccgtcttgtc tgacttcgac tttttcaatt
     6601 actttttctt ctattttctt ttgatttttg ttattatatg gcttattgta atttttacca
     6661 taaacttctc tctttataga ctcgacatta atatctattt gattgcttaa atactttgta
     6721 taaaaatcaa tttcaacagg acttgtcaat tgttttataa ttttagaagc ttcttttgcg
     6781 aacttcactc gttcttcatc tttttgaata ttaaattctt ttttaagatg atctatttta
     6841 tattttatgt agtgagtaga aacatccata gcttttttat aatcactaag tccgtatttt
     6901 cttacaaatt catctgggtc tttagcatct tttaagtcta acacttttac attgatacct
     6961 aatttagtaa gtatatctat agctcttaaa gtcgctttta ttcctgcttc atcagaatca
     7021 taagatataa tagcagtgtc cgcatatctc tttattaaaa tgccttgttg ttcagtcaag
     7081 gctgttccaa gggttgctac tacatttttt ataccatact gataaagtga aattaaatcc
     7141 atatatcctt caactaagac tatagtttta ctttctaaat tttttctagc aaaatttaat
     7201 ccgtataagt tctgtttttt attaaaaatt aatgtgtctg gagaatttaa gtattttggc
     7261 aaagaatcgt ctaatactct tcccccaaat cctattatat ttcctctata atcgaatata
     7321 ggaaacataa ctctatttct aaatttatca tagcaattag ttccatctct attttttgca
     7381 attagaccgc actccaataa gtcttgtttt ttatatcctt tactgattag agtattcata
     7441 agtgaattcc atgaatccaa agaaaatcct aaaccaaatt tcttaataat tttatcatct
     7501 agtcctctta ttcttaaata ttcataccca agatttttgc ttcccaataa gtttgaaaga
     7561 taaaatctag ctgcttccgt atgaatatct tgaaatttct tagatttttc tattttaatc
     7621 ctagtttctt cattcatgtt agtgttaatt tctataccgc atttatttgc taaaattttt
     7681 acagcatcca taaaatccaa attttccatt ttcatgacaa aatttattac gtctccacct
     7741 tcaccacaac caaaacactt ataaatctgt tttgacgtat ttatataaaa agaaggtgtc
     7801 ttttcgccat gaaatggaca taatcctttg taattagctc cagattgttt tatattcata
     7861 tattcagata ttatacttgc tatatcgcat ctagctttta tttcttcaat aatgtctttt
     7921 atatcattca tattatcacc ttctggtgta attttttgtg ttaacatttc tattctacac
     7981 tatattctaa atttgtcaat cactcaatat acacaattcg acttttttta ttgatatcct
     8041 tcttattaat caaaaaattt ttatttttta tattaattct atcaatatta aatctttatt
     8101 tatattaaat aatctaacta tcaaattata acttataaat ttaccttagc aacgattacg
     8161 aataaaaaag aggctcttgc ctcttttaat aattattaat tgatttcatt aaatcttcaa
     8221 attcattaaa gcaaagagat tgagcaccat ctgacaaagc attttctggg tcaggatgta
     8281 cttctaccat tattccatct gctccacatg caaaagatgc cagtgacata ggttttacca
     8341 aatatctaac tccagtggca tggcttgggt ctaccacaac aggaagacct gtttcctttt
     8401 gaataatggg taccgcagct aaatctaatg tatttctagt ataatcatta tacgttctta
     8461 tacctctctc acacattata atattactat ttccttctat tgctatatat tcagaagcac
     8521 ctatccattc agtaattgtg gatgctattc ctctttttaa cataacaggt ttattttgtt
     8581 ttcccacttc acttaaaaga gtaaagtttt gcatatttct tgacccaatt tgaattatat
     8641 cacttatact atacagttcg tccatatctc ttacatccat aagttctgta ataacagcca
     8701 taccaacttc gtctttaacc gcttttaata tttcaagacc ttcttttttt aatccttgaa
     8761 atgaatttgg cgatgttctt ggcttgtatg ctccacctct taaaatgttt gctccctgac
     8821 tttttacaaa tttagcagtc tccaaaagtt gctcataact ctctattgcg catggtccag
     8881 ctattataag tttatctttt tttacagtta tctttttgtt atttgtattt atctctatct
     8941 ttgaattatt attaaatagg ttttctttca taacctacaa gctcctttac ttcttctctt
     9001 tcttcagcag ataggaggtt taaatgttct tttattgttt tttcatttat tataagttct
     9061 tcatttaaat ccataagtgg tatcaaaacg aaagctctat cttgaatcct tggatgaggt
     9121 attgttagtc tttcatcagt ggaaactaca tcattaaaaa atagtatgtc tacatctata
     9181 gttcttggtc cccaatgaac tattctctct ctgtgtagtt ctctctctat ttcttgacaa
     9241 tattctaaca actcataagg ttcaaactct gtttctattt ctacacacat atttagaaaa
     9301 tctgcttgtt ctgtatatcc ccatggtttt gtttcgtata agcttgattc ttttacttta
     9361 tatatagaat cactattttt caaaagttca catgctcttg ataaattatc aaatctatca
     9421 cccatgttag tacctattcc caaataagcc ttattcatct cttgttctcc ttatctcaac
     9481 accaaaatag tcataaatac catttacagg agcctcaggc tttctaactc tgaccataac
     9541 tccatttata agtataaatt tattcaagac ttcttcagca atattttccg ctaaagcctc
     9601 caaaagatta aattgtttat tttcagtaat acctttaaca acattgtaaa cctcggcata
     9661 gcttacagat ttatttatat cgtcactaag acctgcttct cttgaatcaa tataaagttc
     9721 catgtcgaca aagaatttct gacctagaaa attttcttct ttaagaacac catgatatcc
     9781 atagaatcct agattactaa gtaatatttt atccattata ttatacctac tttctatata
     9841 ttgcatcagt cattctagct gccattaaat tttcataaac atcatgtact ctaactatat
     9901 ctacgccatc tcttatacca agaacagtag tagctattgt accttctaat ctttttttag
     9961 gctcaactgg aagtactttt cctattactg attttctgga tgtcccaagc aaaataggat
    10021 accctaaatc ttttaattca tttaatcttc tcaatacttc aatattttgt tcaaaatcct
    10081 tgccaaatcc tacacctggg tcaagtacta ttttttcttt ttttacacca caactcattg
    10141 ccatttctat acttacctta aaaaagtctt ttattgactc cattatatct ttgtcatagt
    10201 ttgtcccatc ttgattatgc attatacaca cttctgcatc atattttcct attacagaag
    10261 ccatattttt atcttttcta agaccccaaa catcatttac catagtgaca cctaatttta
    10321 atgcttcttc tgcaacatct gccttataag tgtctattga tattggtata tccaattcct
    10381 gtttaagttt ttttattaca gggattactc ttcttagctc ttcgtctgag tctacatagc
    10441 tatgacctgg gcgtgtagac tctcctccta ggtctattat atctgcacct tggtcaacca
    10501 tatcttttgc atgttgtatt gctatgtcca agttattaaa atctcctcca tcagaaaaac
    10561 tatctggagt tacatttaat attcccatta tataagttct tttaccataa tcaaacacaa
    10621 ttaatgacac tcccttaaat taaattttat ctcaataaat gattatatct ataataaatt
    10681 gctacttcat agtaatcaaa ctcatagcct cggctctaga cgcaatgtcc ttttcaaata
    10741 ttcctctcac tgctgatgta acagtttttg aacctggttt ttttacgcct ctcattgtca
    10801 tgcacatatg ttcagcttct actactacta agacaccata aggttgtaat tcttccatta
    10861 ttatatctgc agcattttta gtgattcttt cttgtaattg tggtcttttt gccaatgtat
    10921 ctattactct agcaagttta gaaagccctg taagtcttcc tccttttggt aaataagcaa
    10981 tatgagcttt tccaaagaaa ggaactagat gatgctcaca acatgaataa aatggaatat
    11041 cttttaccaa tactaactct tcatgttttt catctgcaaa aagaactttt aaatggtctc
    11101 ttggttcttc actaagacca gagaatatct cttcatacat cctagcaact ctatttggag
    11161 tttcgattag accttctcta tctgggtctt ccccaattgc ctctagtatt tctcttactg
    11221 catgttgtat cttttcttta tcaactttat tcatttaaac tccccccatt gatttgtgac
    11281 tagaagatta gttttataac ctcttttgtc tcaatttctt taaaaatata attataaaca
    11341 tctaaaagtc tttgatagtc ataattttca cttacttttg acactagttc aaaaagctca
    11401 ttattttctt tttttaaaac ttttagcaat tttttgtctt ttggtaccca tttatcattt
    11461 atattaaagt acccaattat tatatctttt aaatataaat tcgtcaaaaa atgatattca
    11521 aaaacatcaa atttttcttt attttttaaa ttcgatattt ttgctccaat atcttgcttt
    11581 ataaaagact tttcctctaa tgacatttta ctgggtcctt ctaagtaaat atttctactt
    11641 atatctttta taatatgtga tatattctcc ctatcaaaca gtactttagc atctttcatt
    11701 tctttcacaa agaaatattc cctatttgat aataattttt ttacaccatc ttttgaaaag
    11761 taatttacat caaattctat tccctttttt ttaaacagta ttctctcttg tttttcccct
    11821 tcttttacaa aaacgaacac atcaatatcg tttatctcta catcataaag agtaaaatct
    11881 acgtctttac tagaacccac taaatataca gccaaagtgt tttggttttc tattatttta
    11941 gttatctctt cattgtatac ttcataaata caacctatca tcttttgtct ccaacaaatt
    12001 gttaatattt taatttattc taataacttc actattttat caaaaacaac attttcttta
    12061 ttaaatttta ttttgtcaat ttctgtaacc tttatagcac ccataagact atttgtgaca
    12121 aaaacaaaat caaaactact aatttctgat atattaattt cattttctat caactctata
    12181 tgtaattcgt cacaaatctc tataatcctt ttttttatta ttccatttaa tatcggtaat
    12241 ctatcactag gagtgtacac tctttctcct tttataaaga aaatattgct catagaacat
    12301 tctaatatta caccttctga atttaaaaat ataccatcat tataaccagt cttatttgcg
    12361 atatttttgg tatatatgtt ttcaaaataa ttagtagtct tatgtctatg aattaaacta
    12421 ttccccctaa ctattggaga tatagctaat ttaaacccct tctcatacgt ctcttgatta
    12481 tatggaatat ccctaataga tatattataa ccttcatcaa aaacagtaat tctaagtgct
    12541 ttattaatga cattttcttt ttttatatat gtaacaattt catttatcaa gaaatctttt
    12601 ttatagttta tatctaaatc caaacaattt attgagctta gcattctttc tatatggata
    12661 tctaaatcta ctgctattcc atttttaact ctaatagttt caaacaaacc aattccaaat
    12721 ttacttaaac tactatcaaa acctacatta ttcttcataa tatcccctca aagccttcat
    12781 tatggattta gctttatcaa gtgtttcttg atattcttca tctgggtcag aatcccaagt
    12841 cattcctcct ccaacttgaa agtagacttt tttgtcattt ttgattattg ttctaattgc
    12901 tatattaaaa tccatatcac cattaaaacc tatataacct attgaaccag tatatacatt
    12961 tctttgcgtt ggctctaatt cgtctataat ttccattgct ctaatcttag gtgctcctgt
    13021 tatagacccc cctggaaaag ttgcttttat tacatcagta gcatctttat catcttttaa
    13081 ctcacctaca actgtggaaa ctaagtgatt tacatttgca tatggttcaa ttacaaatag
    13141 ctctggaact ttaacacttc cagttttaga tattctccca atatcatttc tttctaaatc
    13201 tactatcata agtagttcag ctctatcttt ttcactattt ctaagttctt gttgaagtct
    13261 taaatcttct tctttatctt tccctcttgg acgagtacct tttattggtc tagtttctat
    13321 tctcttatta acacatctta taaatctttc tggagaattt gataatatat gtgcatcttc
    13381 aaagtttaag aatgccccaa atggtgctgg gctaaatctt cttaagtccc tatacaattc
    13441 aaagcttgta agttctgttt ctccactgaa cctttgtgtt aaattagctt gatatatatc
    13501 tccttgtctt atgtactctc taacactttg aactgcattt ttaaattctt cctttgtaaa
    13561 attagattta agtcttatag gtgttacttc tttttcttca taacatatgc tatctatacc
    13621 ttttttctct tcttttaata ttctttgttc tatatcatct attatctttt cttctaattc
    13681 gatatctatg tttggagtag ctatataagt tttttgcaca agatggtcta taactattac
    13741 atgattataa aatccaaaat acatatcagg catctcaata tcatctacag cagttctagg
    13801 taaattttct atgtaattac ctaaatcata agataaatat cccacagcac ctcctacaaa
    13861 tggaagatta gtatcattta caaccctata cttatgtagt tcttctttta aaacttctaa
    13921 aggattttca tcagtatctt tatactttaa taccttaaat ggttggctac ttataaaaga
    13981 atatcttccc aatttttctt tatccatagc actgtctaat ataaaactgt catgttcatt
    14041 tctaaaaata gtaaatatct caaaagaatt taatttagta tttatctctc ttatcatatt
    14101 acatatgtac cctttctcta gcttctgtta taaaattttt aagcatttca tgaccatact
    14161 ctgacaaaat agcttctgga tgaaactgta ctccttctat taaatatttc ttatgcctaa
    14221 tccccattat ttccccttta tcagtaattg ctgtaatttc taattctttt ggaactgtat
    14281 tggagtcaac tattagtgaa tgatacctag tgacatttaa aggattttta agtcccctaa
    14341 aaactccttt attagtatgg ttgatactat atacttttcc atgcacaggc tgttgtgctt
    14401 ttataatatc tcctccaaaa acatgtccaa tagtttggtg acctaaacaa attcctagta
    14461 taggtttttt ccccttaaaa tgctcaacaa tatctatgca aataccagct tcttttggag
    14521 aacacggtcc tggagataac acaattacct ctggatttag ttcttcaatg tcacttattt
    14581 tgatttcatt atttcttttt actacaactg tttctcctaa ttcttctata tattggacta
    14641 ggttgtatac aaaagaatca taattgtcta tcataagaat catactgtaa atctcccccc
    14701 taaaaattat tttatcaact cagaaactgt tacaaattta aaaccttttt caatcatttt
    14761 tggaataaac atatctaaag cttcaactgt atcattagtt gcataatcat gcaaaagtat
    14821 tatatctcca ttttgtactt tattaattat tttatctgca atgctattag ctcctggatt
    14881 tttccaatct ttcacatcta cacctgtcca taaaacaact ttatagtcat gcttttttgc
    14941 aatttctatt aattcttttt ctctataact tccaaatggt ggtctaaata atgtcggttt
    15001 ttttccagtt acttctttca aaatgtcttc acacttaaca atctcctctt ctatctgact
    15061 agaacttaaa ttacttatat caggatgatt gaaagtatga tttcctatct cgtgaccttc
    15121 tttacttgct cttactaatg gttctttata ccattttgca tgttttccag ctataaaaaa
    15181 agtagctttt acattgtatt tctttaatac atctaaaact tgactagttt ctttaggatg
    15241 aggaccatca tcaaaggtaa ttgctattag ctttttttcc cttgaaccat gtttaatcaa
    15301 aacatttcca ttagtatcca tcatcaaagc agaaacttta ctaaaattag atattaacac
    15361 tattccacaa aacaagacta ctgctattaa gcacttatat actgtctttt ttttccatga
    15421 tgtcattttt taacttcctt tcaaatattt tagttattta taaaaatttt gagtgtaata
    15481 tgttttatag cttcctccaa atattactcc aactccaata ttattatagt tccccaaaat
    15541 attttttcta tgtccttcag agttcatcca agcttcatgc gcatatattg cattaatctg
    15601 tcctgctgct atgttttctc cagctgaagt atatactata ccttctcttt tcattctgtc
    15661 aaatggagtt tcattcttct tatttacatg gtcaaaaaaa ttattatctc tcatatcttc
    15721 actatgtttt cttgaactca aagttgcctg ttcactgtat ctcaatgagt ttaaccctct
    15781 ttgatttcta acactattta caaggtctat aacttcaagt tcaaaacatt ttttaagttc
    15841 ttcgctatca tctggatata aatttttaaa ctcatcttca gtgcttttat caattattaa
    15901 atatgaacaa actctatttt cttcatgaat atcgtagaaa attgtaatat acttaccttc
    15961 cttacttatt atatcatatt gattttctga gtttattata tatctggtat ttccttttct
    16021 cttatatttt aatggtgtaa tcttagttct cactgtttgt ctatcttgat ttagttttat
    16081 atcttgattt tcacatgaat taatagaatt actataaagt gctactactt catcattttc
    16141 aagtccaacc attgcaaatt cttttccata ttgattatat acataccaat caaaaccata
    16201 ttcactaata tcaattctac caggtttgcc gatacttgat aaaacatagc ttaaattatc
    16261 tcctaagctt attttttcaa aattctttat atctgactct cctaagttct ccccttcagt
    16321 attatttgat tgtaaatttt gacttttttc ttttttatct ttcttagaat tatcatttat
    16381 atttattttg tctaaaaatt cattccatat ttcactttga aaaaactttt cttttaaatc
    16441 atatccttct tttaataatt caggactata aaataaaaca ataagtacta ctaacactaa
    16501 agcaattaat ctcttcaata tatctctcct tcttaaaatc aatcaaaatt ttttaatatt
    16561 gcatttatta agttaatatt gatagtgtat actaatcaat atcattcata cctatttaca
    16621 gtaagtatta gaaaaaagct ttatctacta attagataaa gctacagtct caaggaaaat
    16681 atttcatcta atgttctatc aaatatagat ggcttatcaa atttccaagt cccttcataa
    16741 accaattcat tatttagata aaaagttcct tgcccatgtt ttttaccatg cataaattca
    16801 cctatatact ttttaccttt ttcccatata taagttccct taccatgaat caaattattc
    16861 ttccattctc cttcataaag tacttcccct ttactattat agcaaatacc gtacccttcc
    16921 atctttccac ctttaaattc acctgcatat atacatccat cactccactt ataaatacct
    16981 tttccatctt tttttccgtt tttccaagtt ccaatatatt catctttact actatataac
    17041 atatttccaa taccgctcat catatcttcc tcaaaataac ctatatattc atttccattt
    17101 gaaaatataa agtttccttt tccatttttt ttatcttctt taaattctcc tatgtactcc
    17161 ataactgttc cttcatctac aaaaaaagta tatgagcctt tcccctccat ctttccctct
    17221 aagaatttcc ctacatatac atccccattt gaaaatttat agattttatt ttttggtttt
    17281 tttgtatcga catcatctat atcgcgtatt gtttttcctt ccaattctaa tacttcattt
    17341 tccggagcaa aatcgcccct ttttaaaaca cgtttcttca tgttctttat acttctaatc
    17401 tctttgttta cttcatttat atatttcaaa taattatcat acaaattaac atattcttta
    17461 gataaattca taatgccatt cctccaacgt tattcctaga tattttacta aatctattaa
    17521 atttattata tcctaatagt ggtcatgttt tctacatatt aagtaatttt ttcaatttta
    17581 attttaaaat gatatctttt aaagtgatac acaaatcatt aataaaacct ttattttcgt
    17641 atactaaatt taaataagct gagagatata atatacaaat ttacattata aactcccagc
    17701 ttgtaaaatt ttctataaat cttttgcttt aaaccctctt ccttttactt ctgtaacatc
    17761 atttacagat ataaaggcct ttttatcata cttcaaagct atatctttta tctttggtat
    17821 ctcatttgat gaaactattg tatatataat cttctttttt tgatgagtgt atgcaccttc
    17881 tgcatctaaa aaagttacac ctctaactaa actcttcatt atatcttctg ctattaaatc
    17941 gacttcattt gacattacca taacagattt ttggtaattc attgcatctt taataaaact
    18001 cattgaatac gcatttagaa atagacctat aagagtgtat aatgctaaat ccaacccaaa
    18061 taaaaaactt cctgtaagta ctatcaagcc atttattgct aaagcagtgt ctttcatttc
    18121 tatattcttc ttaattttta gtattgcagc tattatatct gtaccacctt gagaagacct
    18181 agttctaaat acaactccga ctcctaaacc agataatata ccaccatata cactttgaag
    18241 caatatatca tgtataggta taatattact tatttcctga gttgctccta ataataatga
    18301 aaatacaatc atatttacca aacttgaaat acaaaactct ttttcaaggt atatgaaccc
    18361 taagataaat attggaatat tgattaaaaa tgttaataaa cctacattta tatttgtcaa
    18421 atagtttata gatgttgcta tacctgtagc acctccacta agcagatgtg ctggtcttaa
    18481 gaacccattt attcctatag atgtcatcaa catcccaaat agcattgttg aatatcttaa
    18541 aactggatgt ttttgtaggg tattcttaac ttcactcatt tctttctccc ttcaatcttt
    18601 ctaaatcttt agaattttaa aagcttactc ttacatatat tattcctgat gtaatagata
    18661 atatttcaaa taaatttttt ttataatttc ttgaactgtc atctattaca tcatctatta
    18721 tattatcttt ttcatatatg tctagtgcaa tcttttatat tctaatcttt aaaaacgttc
    18781 aaatcatctc atttatgcct taaatgaaac tttttctagc ataatatgat actgcttatt
    18841 atttaacttg caatatagta aatttatagt atttttttct aaatatctat tttcgcagta
    18901 atttatttta ttttagcaaa ttcaagttta gtaattgtct ctttataatt aattcctaaa
    18961 tgaaattttt ctttttataa tttcagtaca tgattttttt atttcattat ttgcattttt
    19021 ttcgttcagt ttttatttta aatttttttt attttatttt gttttaagta ttcaaggtac
    19081 tcgtcttaat tttatttttt atataaaatt cattataatt ggaattagta ttactgtaat
    19141 tgtaccagag actccaatag aaacaccact catagctcct tctatttctc caagttcaaa
    19201 agcctttgta gttcctaaag catgtgcaga tgttccaaga gcaattcctt tagcaactgg
    19261 atgacttata ttccccagtt tgaatactgt agaagcaacc ataccaccaa aaatacctgt
    19321 aaaaattatg gctacaactg ttattgattc tacaccattt aaagaatttg ctaaagatat
    19381 tcccattggt gttgttatag attttggaat tagtgaattt attatactta catctgaatt
    19441 agctattttg ccaattatca atacagatac aaaagaaatc actataccac aacctattcc
    19501 tacaataatc tcaaacatat gctttttaaa taattcaaat tgtttataaa gtggaactgc
    19561 aagtactact gtgacaggac ctaaaaagaa atttagacta tcagctccta tcttgtaatt
    19621 ctcataaggg attttagata tgcttaaaaa caatattatt cctacgattg caattaataa
    19681 agggttacat ataggattac ttgtttttcg ttgaatgtat cttcctaaat tgaaaaaaac
    19741 tattgttact attataccaa atactggtgt ctgtaataca ttatacattt ttttgtcctt
    19801 cttccttctt ttttatcatt ttctccacaa taattccagt agtatacatt actattagtg
    19861 tacttaaaca tatgactaat aaaagtacta atacattttc ccttattagg tctaatgact
    19921 ttataagtga tacacctgct ggaataaaaa atatagccat attatcgagg aaaaatccac
    19981 ttacattttc tatactactc aagtctaaaa ctttaaattg tagtagtaga aataataata
    20041 gcataccaac tatactccca ggaataagta taattccttg tataaaggaa cttatatact
    20101 ctcctattac ccagatagat aaaattatta ctaattgatt gaaaactttc attttccacc
    20161 tccaaaaatt aaagagattg cttcaaatca tatatttgta gttttatcaa caagcaaata
    20221 tctttcttta aaatagaaag ttataatttg acacaatctc tttttaaatt tatttttatt
    20281 ttaatttaat aaaatacata cttggttgtt tcaaaaaact taactatata aacctatttt
    20341 tcacttaaaa tttcttttat ttttgaattg ttatacatac tgtctggaac ttcacttctc
    20401 aaactataaa aagtctgttg tgcatttttt aaatcgccat tatcataata aagcataccc
    20461 aactcgtaat atgaatcatc ataatactgg tcatttttag tataagtatt tatatatttt
    20521 ttgtaatatt ttatagcttc atccttattt cctaataatt tatttgttat tgctaattga
    20581 tatattgact cagaaatata ttttttagtt ttaccagaag aaactatact tttaaaatta
    20641 tctatggctt tttcatattc tttgtttttc tttaaattta gagcttgata atataaatct
    20701 tcctcatcag ctacagttat ctctctttct ggttctttat ttttaacctc atttaattcc
    20761 ttattcgttt tttctaattt tttactagtt tctcctaact ttttatcagt ttcacttaac
    20821 ttttcctctt tactacttat ttggttattc aagttttcta ttctcttttt gctttgaatc
    20881 aaatatatcc ccatacctat taaaacacag ccaactataa tatatggtaa aatacttttc
    20941 tttcttttta ctttttttat ttcttcattt ttacttgtat cttctttttc ttcttctttt
    21001 tcttcttcat ttttttcaaa tacaggatta tctttatcta gctctttaag tttgtctaaa
    21061 tatccttctc tcttttttac attacctagt ttatcatata acaaagataa aattacatat
    21121 ggctctatta gttcaggttc ttcttcaatt atgttctcta aaatatgtat tgactcataa
    21181 tttaattctt catttataaa tcttatagaa tgattgtatc ttcctaaaaa agttttaaaa
    21241 tcttcagatg aaattagatt tacatatttt ctagatatat gattattttc ataacataaa
    21301 gcagtataaa aactctcaaa tgatttagca aaatcacatt ttaataattt taaaagacct
    21361 ctaagattta gtatatcaat gtctttagga tttatctcca gagctttatt aaccatatta
    21421 atggcactag ttatataatt tttatatgct aaatccaatg cattgttata tcttttatat
    21481 gattctaatt ttaactcctt agacgttgta tctgactttg aatttaaagc tccttgcaag
    21541 tttaatagag tttcttcttt ataatttgta gtcacataac tctgttttaa tgcttttaat
    21601 gtttcatcac cgtcttcttc ttctatatta aaaaaagtca aatcactttt attatcttct
    21661 atcaaagata tatctaaaat ttttctaggc atgctttcta tgtcttgttt tatatcttta
    21721 actaaaataa tactcacctc cactctaaaa tatatattta agataacata tttaatatgt
    21781 caagtcaaaa ctatacatat ttttccacca ttctcataat aaaaaaggta atcctcttct
    21841 tttcataaaa aggttacctc tttttcaata ctattaactt ttaatgttga atttttttac
    21901 ttttctatag agtgtagcta tacctatatt taatgcctca gctacttttt tcataccttc
    21961 tgtattactt ccatatttct ttatggcatt ttctatagct tccctctcaa ggtcatctat
    22021 agtcttaact tcttgtttat aaagttcatc tagtttaaaa gatattaaat cagacatact
    22081 ccttacaaaa gcagttaact ccttatcttt tgaaagtatc ttttcttttt gttcttcatt
    22141 aaacgcagct atacccaaga ttccaagtat atgctctccc attttaacgg gagtacatat
    22201 atcagctaac tctttacaat tttctctatt tgcacattta ttacagttct cttctatttc
    22261 tttatttata acataggatt ttccatcttt taaaacaaga gagtacactg acttctctga
    22321 tattgtctct cctattttac ctctgaatat tcctgtacct gctatcctag ttaaatcttt
    22381 tgtaactaaa gtaacatcta catccaagac attagaaata ttctcgcaaa tactttgaac
    22441 gtaattcgtt atattattta aattcaaagt aatccctcct atttagattt atatttttta
    22501 ttactctaca taaatcctat tcttgagaac tttaagcttg cggttgtggt tgtgtctcat
    22561 taggcttatt atcagttggt ggtgtttctg gtttttgttg attatttgga ttttgattgt
    22621 ttgtattttg attattctta ttttcgtctg tatttggttt ttcctctggt tttggttcca
    22681 tagtcccaac taatataata ccttccttct taggataata tgaattagct attttttcag
    22741 ttttaatttt tttaccattt gcatcattaa atgttctata tgttgataca gaatatgcat
    22801 ttcttccact tttgtctact ttttctttac ctttaggcat tgtagggtca ttaactcttt
    22861 tcattgtagt ttgtgaaacg ccatctaatt ttgttgatat tgttatattt tgtttatctt
    22921 ctgcacttcc atatatattg caaacaattt gatttcctga aacgtaattt tttatgtaaa
    22981 ctggatgctt tagattattt ttaaataaga aatctattcc actatctgca acagttgcat
    23041 ctcttccttt tggaacataa cttgatggta tagtatgatt ttttacatta acaatttcta
    23101 aacctgcgta taacacagaa ttgtataacg tagatgaaac ttgacaaact cctccaccat
    23161 aatcttcttc catctcacct tccattatta caggtgcact tttatatcca ttttccttac
    23221 ttcttttccc tgtatgttta ttaaatgaga aagtttctcc tggcataagt aatacattac
    23281 ttatattatc tgttgctaat tttatgttta tacttctatt tatttgagaa gttttaaatg
    23341 ttgttgaata cgtacctaaa tttgtatcta tattagcaag ttgctctttt tttacttttg
    23401 ctggagtagc ttttactact aactcatttt tatagttacc ttttttagtt tgattatcaa
    23461 aattctccat actcttagaa atatccattt taagaccatc agaatctggt ataaccttga
    23521 ctttttcacc acttatatca agtgtagcat cttttacatc tacatctatt tctttagcta
    23581 tcttttccat ttctgctttt aatttatttt tatcataatt tatcacaact actaagttgc
    23641 tttttttgcc aaaatttgct gaaatagttt ttgatagatt gccaaagaaa gcactctttc
    23701 tatttaaatt atatgcgttt tctactgttt tatccaggtc agtagataaa tttaaatctt
    23761 tgctctttac tttccaactt ttatcattgt aattaaaatc aacattctct aatttgtatt
    23821 ttttagctaa ttcctgtttt gcttgcgatt tagttaattt tccaatattt actccattaa
    23881 catatgtgtt ttttgctatt ttctcccctt taaattgcat gcacattatg cctataaaaa
    23941 ttattactac taatgctaca gctccaatag ctataactag ctttttgttc acattactca
    24001 tcccatactt ctccttacaa taaaattatt tatatatccc tattattata ttactacata
    24061 atgcgcctta aaaccccaaa aactttttaa ttatatatta atttaatatt attaatattc
    24121 atttagtcca ttttctgata tggcatatat tttttcattg taaatattaa tagcataaac
    24181 ttctttgcct ctaaataagc ctttatttac tacagcataa taataaatat ttttatattt
    24241 tccaaacaat tcttctaaac tctttttact ttttacatat ctatctgctt tctctatatc
    24301 taatatctca atctttttat acttattttc tttttcatca agagtattag ttaacacaaa
    24361 cttagcttta tcttttgtaa taccaaattg ataacttcca tcttctaata ttctaaagta
    24421 tccatcacca taatctactt tgtaatcttt tttaccatct ttatatgaat ataaatcttt
    24481 agtttttgca cttaccaaaa cttcatttat taatttatca tactcatata cttcagcata
    24541 atagtattta ttatttttta tcatttgtgg aagtgaattt atagatagat ttgccttgtc
    24601 tttgccactt tttttagcta ttacatttat aatgtcatca gatgttaaag tatacaatga
    24661 taaattcttt ttatattttt tttctaaact ctgtaccttt tcatcatctg aataatcgtc
    24721 ttttaaatac tcaatatatt gtaatgctct ttcataatct ccagaattat ttgcctcttc
    24781 agctctactt atatagtaat catacatttc ttcaatacat tgttctttcc cattttgagc
    24841 catactgtaa tacttttcat cttcttttaa tactttatta tacttttcaa tagcttcata
    24901 atacttatat gctttttggt cttttactgc tgagtcataa acatctctag actctttgat
    24961 tgtttctata ttctgtttgt atacatctag attactaccc ataccattta atattgaaac
    25021 agtacttatg tagttaatgg caatatcata agtagtattt tcttctttat acatttctct
    25081 aactctatca gcttgagcta ataagtcatc cacatctata cttattttgt ttaattcttt
    25141 cacggtattt attaatccaa taaaactttc ttgacttatg tctccatcta aatatttatc
    25201 accactattt aatagcaatt tatttatttt tttagaaaca gacttattaa acctttccat
    25261 ttttgaatca gaaaactttt tttctatact tgtgtagtaa tcaacagcta tagaatactg
    25321 tcttttctct ataatcttta acaactcatt agcttcattt cctacactaa actcgttact
    25381 acttaagaaa aatactatta tcaaagcaat tacacctaat gttgataatg aaatcgcaac
    25441 tatagatttc cttttttcat caataaaatc tacagccttc tcaaataaat ctctaaaatt
    25501 cattatagtt ccctctccat ttaataatct ttatatttta tggtaaacta aatttcttat
    25561 tggttattat atcataattc ttgaaaaaaa ataaaaacac aagttaaatc gtgtttttat
    25621 tttacctatc aatcattatt ttatacataa atatttcctt tacataaatt atgatatctt
    25681 tctttttata tccctagaat gactcttttg ttttttatta ttactgacag ttttcgttct
    25741 tgtcttgtgt atagctttgc tatcaaatat ttttctagca ttgcataaaa caattccaat
    25801 tgttaaacaa actgtaagca cacttcccat caaaaagtaa taatccaatc ctatatgtac
    25861 tcccataact gaatttatta acactaatgc aaaagtcatt gcaaaacata tcatcatgga
    25921 gataactaaa attcttagaa tttttttcaa tccactcacc tcgtataatc actaaaattg
    25981 acactacccc ttatccatta ctataccaaa ttagtaaaaa aatagcaatt actattgtca
    26041 tttatattag gaaaatatta aatcttttta ataaatatct ttttttgaca taaggattac
    26101 ttaactttta ataaaatagt aacactaata tgtattaatt aatagaataa aataaatgat
    26161 aatttattga ggggtgattt acatgccaaa tttgccaagt ttagggtcaa aggctcctga
    26221 ttttaaagcc aatacaacaa atggtcctat tagactctct gactataagg gtaattggat
    26281 tgttttattt tcacatcctg gtgattttac accagtttgt actacagaat ttttatgttt
    26341 tgctaaatat tatgacgaat ttaaaaaaag aaatacagaa ctaattggtc taagtgttga
    26401 tagtaacagt tcacatttag cttggatgta taatatttct ttacttacag gtgtagaaat
    26461 tccatttcct attatagaag atagagatat gagaattgcc aagttatacg gcatgatatc
    26521 aaaaccaatg agtgatacat caactgttcg ctctgtattt attatagata ataatcaaat
    26581 tctaagaacg attctttatt atccactaac tacaggaaga aatattccag aaatacttag
    26641 aatagtagat gcacttcaga ctagtgatag agataatata gttactcctg caaactggtt
    26701 tcctggaatg ccagtgattt taccttatcc taaaaactat aaggaattaa aaaatagagt
    26761 taacagttgt aataagaaat attcatgtat ggactggtac ttatgttttg taccagataa
    26821 ttataatgat gaagaagtga gcaagaaaat tgataatact tgtagctgga aaaaagaaca
    26881 tactaaaaac attgaaaatg aatgtaactg tgaacatgaa catcatgact acctgaacaa
    26941 agctcttgat tgtaaacaag aacacaagac tgatattaaa gatgattgca atcatgagaa
    27001 aaaacatact aaaaatacta acaaagttca caactccaaa caagataagt ttaaagataa
    27061 gtcttgtgat gaaatgaatt ttaactatga caaagatgaa tcttgcgaca aaataaattc
    27121 tagctataac aaagaagata gtagttatga agatttctat aaacataatt ataaaaacta
    27181 cgattatact agcgaaaaaa atactaaaaa aatagctatg aaaactttaa aagattcaaa
    27241 aaaattagtt agaccacaaa taacagaccc atacaatcca atagttgaaa atgcaaactg
    27301 tccagatata aatccaattg tagcagaata tgttcttgga aatccaacta atgtagatgc
    27361 tcaactatta gatgcagtta tatttgcttt tgctgagata gaccagtctg gaaatttgtt
    27421 tattccttat cctagatttt taaaccaatt acttgctctt aaaggtgaaa aacctagctt
    27481 aaaagtaatt gtagctattg gaggttgggg agctgaaggt ttctctgatg cagcattaac
    27541 acctacatct agatataatt ttgcaagaca ggtcaatcag atgataaatg aatatgcttt
    27601 agatggaata gatatagact gggaatatcc tggaagtagt gcatctggaa taacatcaag
    27661 acctcaagat agagaaaact ttacactctt actaactgcc ataagagatg ttatagggga
    27721 tgataaatgg cttagtgtag ctggaacagg agatagagga tatataaatt caagtgctga
    27781 aatagataaa atagctccta taatagatta ttttaatctt atgagttatg attttacagc
    27841 aggtgaaaca ggcccaaatg gtagaaaaca tcaagcaaat ctttttgatt cagacttatc
    27901 tttgccagga tatagtgttg atgcaatggt gagaaatctt gagaatgctg gaatgccttc
    27961 tgaaaaaatc cttctcggta taccatttta tggaagatta ggtgctacta taacaagaac
    28021 ttatgatgag cttagaaggg attatataaa taaaaatgga tatgaatata gatttgataa
    28081 tactgctcaa gttccgtatt tagttaagga tggagatttt gcaatgtcat atgatgatgc
    28141 tttatcaata ttcttaaaaa ctcaatatgt tcttagaaat tgtctaggtg gtgtattctc
    28201 atggacatca acttatgacc aagcaaatat actggctaga accatgtcta ttggtataaa
    28261 tgaccctgaa gtattaaaag aagaacttga aggtatttat gggcaattct aatagaatgt
    28321 agtaaaattt tatagttata taaaaagcag attttattat attaaataag ttgtatctaa
    28381 ttaatactat tattaattgt ttataaatta gatacttatt ttttcataat acaatctgct
    28441 tttttaatca aaatttattt aattataagt tgtttaatta taaagtagga atttagatat
    28501 actcttatac catgtaatta tgtataaata tttttttatt atacaccaat agctttttta
    28561 gctaatttat cagcttcctc attatactta tttccagaat gagctttaac ctttgtaaat
    28621 tttacattaa gcttattctt aatactgtca tagaaatttt tatatgctat agttccttct
    28681 ttatttgttt tccaaactcc agtacaccac ttttcaatcc cttcatagtc aaagtataaa
    28741 atcaagtttt gaacattatt gtcaatacag tactgcattg caattttaga agcctctatc
    28801 tctccagcta catttctcat acttactaaa gttttctctt ttcctttttc actataagtt
    28861 tttataactt cattattttt taaaatcaca acacccgaac catacatttt tactgaatgt
    28921 tcataacttc catctacata tgcttctata aactccttat cttcttgaaa actcaatttt
    28981 tctgacccat caatatattc tttagcttct tgaaaagtag aaaaactctt atattcagct
    29041 cctgagaatc catttacttg ttttttgcac tcatcccatg tattatatac acctatattt
    29101 ttaccttttt tcacagcata aaacttttgt tttgccaaat atctcactcc cactcttaat
    29161 ttttatcttc ttctctcata aatatatcta aatttataag atagtttcta tcaaaatatt
    29221 ttactttcat atcttttttt ataatatcaa tatggtacac ttctcctttt acagctcttt
    29281 gtcttatatt ttcatttata tcttttttta taatatcttt atcaacatca ttgttatcca
    29341 ttttagcatc aacattcaac gcttttatag tatcaaatat aacagatttt agtattaaac
    29401 ttctagattt atttaaatgt cgccttatat aagtataaaa ctcattgcta ttaacctttg
    29461 aaattttaca atcgtgtaaa tctttctcta atctctttaa aacacttata ttagttccta
    29521 catcaaaaat cacaaaacat ccatagtcat atttgcttct taaaattctc cccatagctt
    29581 gctttacctt tatggtcatt tgaggataat ttatagtata gtagtctata ccatacttct
    29641 tcattatagt aaaatacaaa gggtcttttg gatttagatt cggaagcttg tctaaagtta
    29701 cacaaaccaa accatcccct ggaatgtcta cgccttcaaa acatcctttt gagccaagta
    29761 ctacacattt tctatttaaa tcattaagat gtctaatgcc ctttttatca gcataaattt
    29821 ctaagttaaa actatgtaag tattttttca atatttcata agttttttct tgtctatctt
    29881 tactattaaa aagagctaat gtatgacctt ctgtaatctt tgatatatta gatataattt
    29941 tactcatctc atttggaaat tcagaatttc tgtactcaca aatatcagaa aatccaacta
    30001 cagagactct atttttataa tcaaaaattg gttctataat cttttccaca ttcttaactc
    30061 tgtctattcc taatgtattt ttaaaataag acatactttc agataaactc aaagttgctg
    30121 ataaaaatat tcctttttct aattgagata aaatattttc ttcaaataaa tctgctattt
    30181 ttaatggcac tactcgaaat tcaaagacat tataattctt atcaatttca actatccttg
    30241 cataatcatc cttttcatca tactctaaaa agatttcaaa tataatcttt atatcctcta
    30301 aatctcttgt ctttgccttt ccaaatttat atatatctgc ttctttatct atactatcat
    30361 catcaatatt cctataaatt attattatta ttgaaactaa attttttatt attttctcac
    30421 aactcaactt tatcttttca ctataggctc tataacttat ttcagtatct ataccatcct
    30481 tttttagttt tcccacaatt tcatctattt gcaaattcag ttcccatctt aaattataat
    30541 tacttacatt attatattca ttacaattac caaaatttaa tattgaatct atctcttcaa
    30601 taatgagatt tatgcttcta gcaatttttt gtttatcttc tcttccaatt tttaatacat
    30661 tataaaattt atcaaaagcc tttatttttc tcatatttct actatatttt ttatatataa
    30721 agctactatt ttgaataaac tcgtaaggat aaatttcttg taataaatac cttaaagact
    30781 ttgaatttat aatgcttgaa aagaaatcat atcctttttc agtcaaatta tgtgcttcat
    30841 ctactattat attttctaaa ggtttttcat ccttatacgg ccatttagct aatagtgaat
    30901 gatttactac tgttatatgt tcttctttta attcttctat tctgttttta tataaacaat
    30961 cttttttgca ttttttaggt ctgcacatat ttgggtcaca acttacattt cttaagtgag
    31021 tacttatttc cttgaagttg tcaaacacta aataatttat ttcttctata tctccatact
    31081 tgccaccttc aactaatctc ttcaaaaata ttaaagataa aatttctcct ttagtagggt
    31141 tttgagactc ataatcatct atatatgctt ccagtctatc tatacaaata tagttatttt
    31201 ttccctttat atatccgtaa cttacttttc catttagacc caaagaatct aacacatttg
    31261 gaatatcttt atttattaat tgtatctgca attcctttgt gtcagtagaa attaataatc
    31321 tttttttatt aattctagcc tctaatattg caggcaataa atatcctaca cttttaccta
    31381 taccagttgg tgcctctata catgcaattt tttcttcatc ttcgctattt ctaaacaact
    31441 ctcttattgt tttagtaagc tcatattgac caggtctata ttcatatgta aatccttctt
    31501 tattctccca aatagttttg tattttaata attcttcata atttttttct tcttcattta
    31561 aaatttttaa ggtttctctt tccttcttct tttcttcttt tgagtcaaat atattatatt
    31621 ctttttttat atttatatta tttgataaat cataatttgc ctcttctaaa aatttgctcc
    31681 attcccatgt tggaagatta aattttttaa gatatgaatt tattttaaaa ctcaatggct
    31741 ccaaagtagt tttttcatag ttattaaatt ttactaaaag gcaatttaca atatttatta
    31801 tatcaatagc atctgataaa gctctatttt ccactttaga attacaattt gttaaagtat
    31861 tttttaagta ttctaagcta taatctttat gatatggttc taaaataact gctaattcaa
    31921 ttaaatcaat aaatttgttt tttaactttg gcatatgata ctcaaaaaac ttttttttca
    31981 gacttaaatc atgacatata atgaaactat cttctacaaa atttacaagt ctatcttcca
    32041 ccagttctat ttcattagct atttctaaat catttttatc taaattttta catactgaga
    32101 atacttctac aggtacctca aatttgtttt ttattaatga ttcataggta tatattttcc
    32161 agtctttaac cttgacagct ccaacttcta aaatttctga atttaaacaa tctaatccac
    32221 taacttctat atctaaaaaa acaacatcat tcaatacact tattatatta tccattttta
    32281 tatcccttca agttaaacca gcctaactta tttacaatca tttctcttta tattttatca
    32341 tttatataca ctttatttaa gataattaat atcaacttta tatcttacag taaacttctt
    32401 tcatttatta tttgtatatg tgaatatttt taactataaa tattcaaact atattataag
    32461 aaatatattt taaattttaa ataagaggtg aaattgtgaa aaaagatagc ctaaaaaaat
    32521 acattatgat aggagcattt gctctaatac tttttggaat tgctagtata aattttaaat
    32581 cattagataa aacaaaaact caaatttcta gtccaacact cgacacacat gaatatgatt
    32641 ggtattttaa ccctagggag gatggtaaac agccatcacc tataaaagaa gctgattttt
    32701 ttaaaaaata cggagcttat tatgtaggaa atcccaatga aaaagttata tatctatcat
    32761 ttgatgctgg atatgaaagt ggaaacactc caaaattatt agatacgctt aaaaaacaca
    32821 atgctaaagc tcaatttttc gtagttgaaa gttatataaa aagcaatcca gagttaataa
    32881 agcgtatgga aaaagaagga catttagttt gtaatcattc aaaaagccat ccatcaatgg
    32941 caggtattac agattttgaa aaattcaaag aagaaattac aagtgtagaa aaagcataca
    33001 aagatgttac tggaaaagaa atgcctaagt attttagacc tccaatgggt aaatttagcg
    33061 aacaatcttt aaaatataca caagatttag gatataaatc aatattttgg agttttgctt
    33121 atgtagattg gtatgaaaag aaacaaccta ctcatgaatt tgccaaaaac aaaatttatt
    33181 caagaactca tcctggtgca atagttttac tgcatccaaa ttcatcaaca aatactgaga
    33241 tattagatga ggttcttaca cattgggaaa aagaaggtta taaacttaaa acacttgatt
    33301 atttaaataa taaaaaatag tatcataaat ttaaaaaaac tctatcatga attatagtaa
    33361 aaatatagtt atttataaat actttaattc tcgatagagt tttttatttt ttatagctat
    33421 ttaaaaagaa ttgtccattc atttatctct ttgtatccta tattatgata tattttacct
    33481 gctacatcat tattataaaa aagacatgga acttttcctt ctaacaacaa ttctttactc
    33541 aaattgtaaa ccatataact tgcaagtcct tttcccctat atcttttatc tgtacttaca
    33601 gagaccacca ttgccaaaaa actagtttcc ataccagttg acacagtgct aatcatagta
    33661 tcatcatttc taataaaata tgctcttaca tttccctctt ttaaatgaac ttttctttct
    33721 tctatgtaat ttttacttac tttatagtct gacctattta aaagttttcc tatttcatca
    33781 atatcttttt cctgagcatt ttctatttta tactccttca tgttggaaaa atctatttct
    33841 tttatttctt ttaatacaca aaatttatta tctagttttt cataaaaatc tttgtattta
    33901 cttattagtc tatctattac acattttttt ccacttatgc tttctacatc taaatctttt
    33961 atatgattct tcatttctcc tatattaaaa ttatttttaa aactataaat tataagtgtt
    34021 gacttatttt ttaatattat agaggttata tttccatctt ctacctgaga ccatatatct
    34081 tgatattgag agctaaatcc ataattctca acgtatccaa ttatgaataa atttaaactt
    34141 tcttcatgtg atacatattg ccaaaattct ttttctaaag tattatctat ttttacaatc
    34201 aagcagatat cctccttcta tgttaataat gatatacctt ataatttata gtatcattta
    34261 aaatcattat attctaccaa atattctaaa tttttaaata tctatgcaat atgttcgtat
    34321 ttatttttat attctgttac tgcttcactc caagcttctt ctactgaaat acctctctta
    34381 tttgcaattt tttgtgctaa ttctattatt ttcatagctt ttcttgatat attcatatat
    34441 attcctcctc taaattatca taagttattt taaagttaaa tttctatagt catgttatat
    34501 atttatttac ttatatctta ctaattattt atgtactaaa tatgaactac aatagaagaa
    34561 atttagaact taataaaaca taaaatttat atacagttca tatatatttg tcatatcaca
    34621 agacaagtga tataatttat tatcatttta ctgggggtga tatgctatga aaataagagc
    34681 tgaatataca tgtcctcttg aaattgtaca tgacataatc aaaggaaaat ggaaaactat
    34741 ccttatattt gaattaagac aatctaataa aacattttct gaattgcaac ataatattta
    34801 tggaataaat caaaaaatgt tgattcaaca attaaaagag ttacgtgatt ttgggattat
    34861 tgataaaata agttctgctg gatatccatt acatgttgaa tattttctta ctaatcgtgg
    34921 taaaaaaatg ttaaaagcag tggaaataat gcaagaaata ggaatcgaat atatgcttga
    34981 acatggtcaa caagaaattc tagataataa aggaatttgc tacaataagt aatcctatac
    35041 ataccaaaaa gtaagtaatt tacatttttt acaatagtat tataataaaa ataaatagga
    35101 ggtataatca tgtctatagc tttaatattg attgatattc aaaatgatta ttttaaaaat
    35161 ggaaaatgtg aattatttca atcagaagaa actgctgaaa atgctaaaaa aatactactg
    35221 ttttttagaa aaaacaattt accagttatt catatacagc atatatcaac agataaaaca
    35281 gcttcttttt ttcttcctaa tactcatggt tcagaaataa ataaaattgt ttttccacta
    35341 gataatgaag aaattataat taaacacaca ccagatagtt tctttgaaac agatttgcaa
    35401 agtatccttg agaaaaaaaa tattactaat ctagtaatct gtggaatgat gagccatatg
    35461 tgtattgata caagtgtaag aacagcaaag aaacttcgat acgacataac tctaataagc
    35521 gatgcatgta caacgaagaa tttatcttgg aataatatag aaattaatgc aaatactgta
    35581 catcaagtat tcatggcatc actacagaac agttttgcag atgtaaaaaa cacagataaa
    35641 tttttatcta actatcacaa ataaaaacat acactatgtt aaaagagcaa gtttgattta
    35701 tttaagcttg ctctttaatc atttaaaatt agatgctttc tatcattttt atatacataa
    35761 tatacgttta tacattttat attcatatat aactattata taaattttaa actttactat
    35821 attcacataa aaaatgtagt attatagtat atataatatt atatagaagg gggtgctaga
    35881 taaatgaacc atcatatatt gattcaattt gaatatatag caagaatcat tactgctggt
    35941 ttctgtgggg ctttaattgg atatgagaga aagaatagac taaaagaagc tggaatacgc
    36001 actcatttta tagtggcatt agcttcagca cttataatca ttgtttccaa gtatggattt
    36061 aatgatatag tgggtactcc aggtattggt ctagacccag cccgcgttgc agcacaagtt
    36121 gtcagtggta taggtttttt aggtgctgga ttaatattta ttagaaatca atcagtaagt
    36181 ggtcttacta cagctgctgg tatatgggca acagccggta caggtcttgc cattggctct
    36241 ggattatact tagttggtat tgtttcaagt atcattattg ttattgtaca gataacactt
    36301 catagagata ggaaatggat gaaactccct acagcagagc aagtttctct taaaatttct
    36361 aacagcactg attccataga atatattgaa ggtaaattta aaaaatctaa tattgaaatt
    36421 ataaacatga atattaaacg ccttgatggg gattggttag aagtagttat atatactaag
    36481 ttacctaaag gatataaaaa aacaaattta ttaaaattat ttagcgataa tcctcttata
    36541 gaaactttag aaatataata taaaaattga agcttaaaaa taataatata tttaatcaag
    36601 gtcaatatat aaataattac gggatgagtc tatataaaat tcatcccttg ttttttgcca
    36661 ttcatatata ttttttcaag atatttttgt tcatctatta tctagccaag tagctcttgt
    36721 gaactaaact ttttaataca aaaacttcaa tacaaaacta aatataactt ttattaaaca
    36781 ttctacatat atagtttgtt tcttgagtga aaaatagatt tcatattgtt aagatactgt
    36841 taaatatttc aattttctga caagtatgtt gaacaataac ttatcctagt gtattatttt
    36901 agttgtagaa taaaactaaa tcggcaaaac tagagaaatt tagtgacgca aagctatagg
    36961 gactaacgct tataaaataa actatgagtt atgtcagcca gttgccaaaa agatattgtt
    37021 ctttttgttt tatgaaagtt ttttaggggg atagtaatat atatgtttag agatgaaatg
    37081 gataaatgta cacacatgtt aactgcttat attagtagtt tatatgatta ttgtgatttt
    37141 atagatacac agctagatga ttttatacta gagtacggag aaaatgtagt agaatcttgt
    37201 ttacatcaag tgatggtatt ggtaagtaag tataattaaa tagaaattgt tttctagaga
    37261 acccttttta gttttatcta catttaaaaa tgttattata ttaaaaaact aatctcccct
    37321 atatgcaaag tttcttccat ataacaattt cttcccattt atactttttc acttatatct
    37381 ttaataaaat tcaacttcaa atattaatat acttcaaaaa ttattctatt tcttatcttt
    37441 acaccaattc ctatcaatat tatcctttat ataggttcta actatttcgc cacaatctaa
    37501 acataaatct gaatatatag tagattgtac tggaaaagtt ttatttataa ctttatctaa
    37561 ttttctatct cctctatatt ttaacccaat agtttcagca tatatgccac cagttgttaa
    37621 aattcccttt tcaatattta cacttccaca attagaacat ttcacattta taacccccta
    37681 aacaattatt ttttcatgct atcttataat ttaaaatata atttgctacc gattttataa
    37741 cacagtgaca aatcggtggc aaatcaacat tttttcattc tatttaccat ctaatccatt
    37801 gaaatatagc tatttagtta taggaacttg aatttccgtt acaaactcct ctacaattcc
    37861 agtctcatgt atatctatct tgtaaatttc tatagggtca gcaattatct tataattgtt
    37921 ttcttctata aatttaaaca ccttctccat atacatctta ttattagaat tactcccagt
    37981 ataatttaaa gttacataat atccttcatc aaaaactata ttatatactt tctcattact
    38041 ttccagaaaa caaaatacag atttaaactc attaaatata cctttattaa ttgcttcagt
    38101 agaaaaggca gaaccaatat tgttatttcc aagtatatta aatctatctt catattcttt
    38161 ctgaagtttt tgaattaaaa aatcgaagtc ttcatccctc tttatatcag catttaattc
    38221 taaagctttt cttttttcta tatatacaac ctctatctca tctatttttg tattttgaat
    38281 tacactttca attgaactca atctttttat aatattttcc ttatggctta taaattcagc
    38341 tattttttta tcaattaaat cgagttcttc atttaaaata tttttcgtag actctatact
    38401 tctgtcatct aggtattctt ttattttctt cattggaaag ttcaagcttc taaattcttt
    38461 tattaaattt aatttccata tatctgatat attatacatt ctatatccat ttatatctct
    38521 aactggtctt agaatgccta tttcttcata atacattaat gaatctctac ctattccata
    38581 tattttagat atctctccta ttttatagta atctttcatt tatataataa actcctccga
    38641 aatcctattt ttattttaaa tcaacatt
//
LOCUS       DBGJFF010000026        37451 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.026, whole genome shotgun sequence.
ACCESSION   DBGJFF010000026 DBGJFF010000000
VERSION     DBGJFF010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 37451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37451
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.026"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            424..1284
                     /locus_tag="ACOIAF_001364"
     CDS             424..1284
                     /locus_tag="ACOIAF_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HGZ2668380.1"
                     /translation="MKKQKSPILLEMLGLFFGCISMSIGINMFLKPHTIAPGGLSGLS
                     LVLNKVTGLPVSAIMLIIGVPLVILAFRIMGTKNSLKTLFGTVVFSAIVQLTDPLSKL
                     RFTNDLLLSSIAGGILVGIGLGIMFKSDASTGGTDLIALILSKKFPGIKATKFMSCLD
                     GMVVISSGIVNRSLETALYSGIALCVLIKIADMIMEGFDHSKAFFIISDEPESLRQAI
                     TEELDRGLTILDGKGGYTRENKEVLLVVVSKKQELYLKRLVKRTDPTAFVIVTDVHEV
                     LGEGFKNLEN"
     gene            complement(1524..2081)
                     /locus_tag="ACOIAF_001365"
     CDS             complement(1524..2081)
                     /locus_tag="ACOIAF_001365"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGZ2668381.1"
                     /translation="MSDSEYPQMKTLETRSCILRPASLDDVEDFFDCYKNKIVVKHLP
                     FSEHKSLEDTRKFIKSFFLNPYKKGKIGHFAIVYKRDNKVIGNMGFNNINPKALEAEI
                     GICINPSYWGHDFATEITKRVIQYGFRELNLNKIIAITYEENQNSTKSLDLLGFKCTG
                     KYIKKIHTGNTVKNVPCYQYELKKQ"
     gene            complement(2271..3158)
                     /locus_tag="ACOIAF_001366"
     CDS             complement(2271..3158)
                     /locus_tag="ACOIAF_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGZ2668382.1"
                     /translation="MNIKLELYKVFNAVVNNKSFSLAAKELFMSQPAVSQSIKQLEEQ
                     LDTLLFYRNNKGVKLTPEGKILSEHVTTALKLISSGEDRINKFKKLEYGSLKIGVGDT
                     AARFFLLKYLEIFHKKYPHIHVSTINRTSRELISLLKDGNIDIAIINMPIEDDTLNIV
                     ECIEIHDIFVCANDYIEYKGRKISLEELNTLPLIMLENKANSRLYVNEYFLSKGIKLN
                     PDIELGSHELLLEFAYINLGVSCVIEEFSIDYLENEKLFKLDIKEPIPKRNIGYCHLK
                     DISLSLATKEFLSMISNNI"
     gene            3277..4476
                     /locus_tag="ACOIAF_001367"
     CDS             3277..4476
                     /locus_tag="ACOIAF_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003493023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HGZ2668383.1"
                     /translation="MDRLVGTVSRGVRAPIIRQGDDLVKIVVDSVLNASKSENFEVRD
                     KDVIAVTEAVVARAQGNYAHVDNIAKDVKDKFGDDTVGVIFPILSRNRFAICLKGIAK
                     GCRKVVLMLSYPSDEVGNHLISIDELDDKGINPWSDVLTEEKYRELFGYNKHTFTGVD
                     YVDYYKNLIKDCGAEVEIVFANNPKTILNYTTSILTCDIHTRQRTKRILRQNGAKKVY
                     SLDDIMTASVDGSGYNDQYGLLGSNKATEETVKLFPINCDEVVNKIQGNIKEITGKDV
                     EVMVYGDGAFKDPVGKIWELADPVVSPAYTKGLEGTPNEVKLKYLADNDFADLSGDEL
                     KEAISKYIVEKDNKSDDLTGNMVSQGTTPRRLTDLIGSLADLTSGSGDKGTPIIYIQG
                     YFDNYTK"
     gene            complement(4670..7225)
                     /locus_tag="ACOIAF_001368"
     CDS             complement(4670..7225)
                     /locus_tag="ACOIAF_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HGZ2668384.1"
                     /translation="MMSLVKFSIQYIKHYKKQSISIVLSIVLSVALLTGIGSLVHSAD
                     KSRIEKIREDSGDYHYFYKVDKEQLNKIKENKKSKEYTVDRLGITKPKNQIDEPFIIE
                     FLNADSSYLDMTGRKLLKGTLPSKDGEIVLDTYSLNNLNVNKEIGTEINLDGKIYKLC
                     GILSSLSNPETALEGFVNDYFQPKEKDSYIVYVKYDENKNIKKQNYKFMKKFNISKEK
                     TYINWPLSVEFGVKPPKNIEKYNIFEFLKNLELNTNAIILLVGVFGAAAVYSIFHVSI
                     LQRISQYGVLEVLGANNKQLLFLLFLELFLLFIVGFPIGCFLGIGVASTIYEQFPHIF
                     LSSDIVPGAFFISEKSIYLGFLFILLLLILIAVRVVHQLNKYSSIESMKNFKITNKKK
                     RILESVKHSNMTKVLSHKYMTQKKGMFIGILFSLSLGGIIFLCSSYSIQLTKNNNELT
                     MKADDGLNSDYQITMQTTDFDVGIPKDKISRLNNVEGISKVYPVRYLFGAMQIKEEQL
                     FWKNFFKPLEKDYRIKTFFDGICTKQNEGGYLLKTNIYGYNNGMLEGLNSYIIDGEIN
                     THDMVKNNKVIVRLPMDGTGMYDAVDINPGDTIKVKVPKTMKPTDEIVKFKEENKNDY
                     TTKEFVVAATVKRVMANNIYFIGDYGMDIVMTNGQMKNNFDIENYNSVSIKKTEDSNS
                     EELSENIKSVVSDVKRCIVTDYTIAIEKNNTYLNQKLLFIYGIVFVLLAISLFHIINT
                     VSYLIFSRRHEFGILRAMGITDNKFLLMMIREGFLYGFYASIIMVIGSVIGQFMIYFM
                     VKRVYLYINPILKINTPLYIGMIILNITISIVAVIIPVRQILKSDIISEINKN"
     gene            complement(7222..7908)
                     /locus_tag="ACOIAF_001369"
     CDS             complement(7222..7908)
                     /locus_tag="ACOIAF_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2668385.1"
                     /translation="MSILVTNNLVKHYGKGETKVKALNGVSIEIERDTFTAIVGTSGS
                     GKSTLLNIIGGLDNPTSGDVIIKGKNISKIGKKDLTVFRRRNIGFIFQNYSLMPVLNT
                     YDNIALPVTLDKGNHIDHEYIEMLMKTLGIWDKRLKFPSELSGGQQQRVAIARALANK
                     PALILADEPTGNLDSKTTMEVVCLLKESSAKFHQTILMVTHNENIAQVCDSIIHIEDG
                     VVVNTGGEVL"
     gene            complement(7985..8893)
                     /locus_tag="ACOIAF_001370"
     CDS             complement(7985..8893)
                     /locus_tag="ACOIAF_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HGZ2668386.1"
                     /translation="MFILAFIICTVVIFIVSTKINQRRYDELNLMLNQILEGKEVTYP
                     DTKDTRASKISHQVKKIKDMIEIEVEQSKSEKEAIKGLISNMSHQLKTPLSNITIYCE
                     LLENINISTLQKKEFLQKMKNETFKIDWLLQSLFKMTKLEDGVIEFEVEELLIKDTLI
                     QSISTIFNKAEAKNIRINLEPFSDIKLVHNKKWTIEAIVNVLENAIKYSPSDSTITIS
                     VIKMELYTKITIKDEGIGIDSRELNDIFKRFYRSKNVENQNGTGIGLYLTRLILEKEN
                     GNIIVESKLGSGCCFSIFLQNCKSLN"
     gene            complement(8895..9566)
                     /locus_tag="ACOIAF_001371"
     CDS             complement(8895..9566)
                     /locus_tag="ACOIAF_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGZ2668387.1"
                     /translation="MNLLIIEDDINLNEGLFYAFENDGFNVFKAYTKQEGLNIFNSKN
                     IDFIILDCNLPDGDGFDVCQIIREKSDIPIIMLTARDSEIDEVKGLEIGLDDYITKPF
                     SLSVLKARVKVAIRKKSNNKVIYSNGIKLDQKLLKVYKNKECLELSSVEYKLLSYLIE
                     NKGQILLKEQILHHIWDSEENYVDDNIVSVNIRRLRVKVEDDPSNPKYIKTAYGMGYL
                     WNEVE"
     gene            9828..10892
                     /locus_tag="ACOIAF_001372"
     CDS             9828..10892
                     /locus_tag="ACOIAF_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reductive dehalogenase domain-containing
                     protein"
                     /protein_id="HGZ2668388.1"
                     /translation="MKRVDERNTMFARANYKKDSTAYNDYYKKNPDKKEIDDSIRNRP
                     NLCSEGTMTYNELNSPMASSAFDFLSDIKSLCEGKVSDTKVDVDAKSMTKKIKGLAKQ
                     YGASVVGITKLKDYHIYTNRGRHEENYGEEINLTHKYAIVFGCEMDKEMINRAPMICE
                     VIETSKCYVDASIVGMILSYYIRNLGYDARNHMDANYLVMPVFIARDAGLGDIGRNAI
                     LTNKDYGSRLRLGVVTTDIPLLEDEYVDFGLEDFCKVCKKCSFNCPSHSLSNDIKIGD
                     DGKYNWVIEHETCYIKWRYLGTDCGMCISACPFSQNLETIKNTTSFKGNNELIQKALD
                     EYTSKFGKRIFIPGNPSWLK"
     gene            11050..11736
                     /locus_tag="ACOIAF_001373"
     CDS             11050..11736
                     /locus_tag="ACOIAF_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGZ2668389.1"
                     /translation="MEKILVVEDDSILNKTLSYNLIEDGYIITSKFTAKSALKSIFEC
                     EFDLIILDINLPDKSGFELCNEIKENYNIPIIFLTANDMECDMIKGYELGALDYITKP
                     FNINIFKQKVKAFLNHLTIKTKQDYYRDGYLEINFSELSANINGNQIIFTPLEYRTLK
                     LLTENPKSILTRKVLLEKLWDIDANFVDEHTLTSVISRIRSKIEKDNLKYIKTVYGIG
                     YMWLGERNEF"
     gene            11726..12757
                     /locus_tag="ACOIAF_001374"
     CDS             11726..12757
                     /locus_tag="ACOIAF_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HGZ2668390.1"
                     /translation="MNFKKFSFSSIYLIIAVGIIFTAILFSISIYLITDDLKLIALLL
                     IYTIVLCGFIALTIFLLHKKIVIFCSKICHTLDSMMNSEDNIEIELEEETILSRINHR
                     LVRMYEVIQENRNSIAIEKADLQELVSDISHQIKTPIANLKMINTTLLNQTFKTEIQK
                     DFLMDMENQLDKLDFLMQSMVKTSRLETGIITLSKKKNSIYETIATALSGILFDAEKK
                     NIEVTVDCDPNLYIIHDKKWTSEAIFNILDNAVKYTNSNGKIRVATECWVMYTKIDII
                     DNGKGISESHQAEIFKRFYREEDVHDIEGIGIGLYLSRKIISLQGGYIKVTSDIGKGS
                     TFSIFLPNK"
     gene            12839..13504
                     /locus_tag="ACOIAF_001375"
     CDS             12839..13504
                     /locus_tag="ACOIAF_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2668391.1"
                     /translation="MSILQVINLKKYYGQSSNITKALDGVTLSIEKGEFISIVGTSGS
                     GKSTLLNMMGGLDNPTSGKVIIKGKELSTMTDEQLTIFRRRNIGFIFQNYNLVPLLNV
                     YENIILPIGLDGESIDKDFINEIINMLSLEEKLYDMPSNLSGGQQQRVAIARALVTKP
                     AIILADEPTGNLDSRTSSDVLSLLKVTSKKFNQTIVMITHNNEIAQLTDRIIRIEDGK
                     IVN"
     gene            13517..15934
                     /locus_tag="ACOIAF_001376"
     CDS             13517..15934
                     /locus_tag="ACOIAF_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HGZ2668392.1"
                     /translation="MFRNNNRTFIRKIALNDLKINKLKTYLSGIIIMISTCLLLTVTL
                     VSYNASVDLVNASPYHAIYKSVDEKAKNILYKNKNFSGVGTYKLIGSNKKTDCIMSIV
                     YADDTAIKLMNFQPLKGKLPTNKNEIAISEKYLQEFGLDKDIGDSIKLNYYDDITNKE
                     VQCDFIIVGFLENYYQDNAKQYYTVVSNDYFKNIASTPLQNKSNTFDESRPDTVDVLV
                     KLSEENTEKDSASIKTQLKKIGLSLGIKKYNIYLNDNYIESNLIDGEQIITVLVVGIV
                     VLFSSVFVIYSIFYISVVNSVQMYAKLKSLGMTSFQLKKIISLQGNILSIIFIPLGVI
                     ASCIIAYIIQPLAWQMIADLFIILILSLVMFLTVRISLFKPARIISKISAIEAMQYTE
                     TKFRKKAKNFSYINIKNLALKNIESNRKKNLIALISLSISGVLFISIANLANSIDFKK
                     QLSQQFVYNEDYIISIKSDNLYEHITEIQQNNPLTDSLKNEIKSIHGVKKLIESKSIK
                     SNIIEPYIKDRDEKQFITLIKGITPELSKSLEEYIVSGKIDYTHLDSDSLIINKYRTK
                     YYGLNLKVGDTVTFNIFSGNDIIKKKMKIIGIIDNSNTGGMFFASDKTLESLTPYNTN
                     LDFSILIDKKHSKEVEEKLLSIIQKNSNLMLYSYEDDYRMITRAFQYIIIASYIFVGL
                     ISCFGILNMTNTLINSVLIRKKEFALLQAVGMTRKQLRNMLYREGLNISIKAICTSSI
                     LGYFGSNLLCTFIKDVIRLDFINFKFSIFTILIFSFVLIGIQVLVTELLVRNIEKKSL
                     TERLRSQ"
     gene            complement(16115..16906)
                     /locus_tag="ACOIAF_001377"
     CDS             complement(16115..16906)
                     /locus_tag="ACOIAF_001377"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGZ2668393.1"
                     /translation="METKQYLIDFYNTYDEDSRLALKHGMVEFLTTMHYIDKYIKSGD
                     CVLEIGAATGRYSHTLARQGYDVDAVELVEHNIEVFHKNTQSNENISITQGNAMDLSV
                     FPDNKYDITLLLGPLYHLYNKEDKQQALHEAIRVTKPGGVVFAAYVISDGCLIDEGFH
                     RGNINVSEYIEKGLIDPQTFAAKSEPKDLFELVRKENIDDLMSAFNVTRLHYVASDGL
                     ALYMREAVDSMDDDAFALYLKYHLATCEREDLVGVTSHAIDIFRK"
     gene            complement(16939..17526)
                     /locus_tag="ACOIAF_001378"
     CDS             complement(16939..17526)
                     /locus_tag="ACOIAF_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HGZ2668394.1"
                     /translation="MPPKVKITKEMILNTVLDITREVGFEAVNARSIASKLQCSTRPI
                     FTCYENMNELKNEFLAFAYEYYNQYVVNYSNSKSVSPYLLLPLSYIEFAQEETHLFKL
                     LFINDLDLMMKEANDFYKEIDNEKKARLFSESIGIELECAKVIFLDLFLYTHGIAVLT
                     ATKKLTLDRYSAEKMLKNILTAFIRQEKPDWNLSI"
     gene            complement(17703..19094)
                     /locus_tag="ACOIAF_001379"
     CDS             complement(17703..19094)
                     /locus_tag="ACOIAF_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HGZ2668395.1"
                     /translation="MERLARREQPHIKFGMFKIRIPFIHYRFEKPEAIQGIISSMTSL
                     GTIGLSTQILGLPYEVAWSMAIINSILYCLHVFMGDPVVPGWITASITLTTAYLLKFS
                     MGIERIQALTALQIDLGIIFILMGITGVAGKLVSKIPNSIKGGILMGVGISTIISEFN
                     PKGRFDSYPISITVGILVACFVMFSERFDTLKVKNKFLFHLGEYGVVSAILVSLIVGI
                     FSKEIEIPRFSFDNLVYIVDFKNLINTVSPFGIGFPSVMLFIQGIPMAFMIYIIAFGD
                     FITGENLVLSESENRKDEYIDFNSNRSNVISGIRNIFMAIFSPYIPMCGPLAATLTGS
                     VAQRYKVGKEAMQSVFSGMGTLVWVSAIFICFYPIAQIATPLIPLALSVTLLVQGYLC
                     TKLSMELCNTGVEKGLIGLMGGVIAAKGGTWGLAVGFILYFILIDSKKRKEKDINSIE
                     DCVYEILDKKKSI"
     gene            complement(19284..20582)
                     /locus_tag="ACOIAF_001380"
     CDS             complement(19284..20582)
                     /locus_tag="ACOIAF_001380"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase/bile acid-CoA:amino acid
                     N-acyltransferase family protein"
                     /protein_id="HGZ2668396.1"
                     /translation="MKVNVYNVESFVDERLKIMVSDLIPNTKLKISVKMEFPWCKGEE
                     FSSYGVFCSNEKGEVDLDLVEPIEGTYKADNCMGLIYSLEKSKTEGKNFAENISIDKP
                     IIMNMIFETSIERKEVELKRIFKSEDITTKYISDEFIGKLFYKQNSNNKTILMLGGSD
                     GNLDALDLLAAPLASRGFNVLTVAYFALEGLPDKLEEVPLEYFEKVFEWISENEITST
                     KEIFVHGTSKGGELAFLLASRYEQIKKVVVSQPHIYCFQALNGLMSGNDTSSWSYKGE
                     PFPYIKVDNDIFFEEQKKNISKDIPFGFNSTYKKSLKRAENRKEARIKIENSKADILM
                     IAGKEDNIWNSYEACLEALEIFEENNYPYNVELLTYNNMGHPLPIPYIMPLKETLCMS
                     MSRGIFSSGGTVEGNSKGQFESWNRTIEFYKKPLSSDLNN"
     gene            complement(20939..21814)
                     /locus_tag="ACOIAF_001381"
     CDS             complement(20939..21814)
                     /locus_tag="ACOIAF_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HGZ2668397.1"
                     /translation="MITFDVNGAIIRFDDTKNNYNKIRKIFKLYALEISKNFERDCLN
                     SFQNLKQISDRGLSLGEKYIEESFKKGIETIVSFGIITIDIDTFKSVYCEKYLDFKRL
                     FNNLNKTFIIPNKNKKNGHASFLEIESTIKKMSNYLYNDCFKIHYAVVDALIENRITA
                     VHSYIDEESIKKSNALFNNYKDGFIGKADECKVVKQIITLNPYREDVYEFLVKEDGDF
                     SKEIERLTEFLGYDIREYKASLMDIYINELMESDENDVENAKEKIEKYARYIGCSDEN
                     LYVARVNAIYTFANA"
     gene            complement(22007..22693)
                     /locus_tag="ACOIAF_001382"
     CDS             complement(22007..22693)
                     /locus_tag="ACOIAF_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HGZ2668398.1"
                     /translation="MFGVIANSLAIAGGCIVGLIIKGGLPQRVSDTIMNGIALCVLYI
                     GISGALEGKNTLVTIISVAVGALIGELIDIDKWVNKLGAFLQSKFSKGNKKDSIAEGF
                     ISSSLLFCIGAMAVVGSLESGLTGSHDTLFVKSVIDGIASIIFTASLGIGVMFSAISV
                     FLYEGIICVGASFLNGFLSDPVVTEMTAAGSLLIIGLGLNVLKLTNIKVANLLPAIFI
                     PILFGVFGII"
     gene            complement(22742..23275)
                     /locus_tag="ACOIAF_001383"
     CDS             complement(22742..23275)
                     /locus_tag="ACOIAF_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HGZ2668399.1"
                     /translation="MSDLNNKKEQDENESSMNETKIYDILDKLNVEYDVVEHEAVYTA
                     EQLVILDGITKGCQCKNLFLRNAKGNKYYLIVVKGEKQVDLNSLKDKIGSSRLSFASP
                     ERLYNVLKLLPGSVNPFSLINDTERKVELYIDKDVIKEDYLNFHPNINTKTVNISRKG
                     FEEFLEYLKYPIQSIDV"
     gene            complement(23319..23951)
                     /locus_tag="ACOIAF_001384"
     CDS             complement(23319..23951)
                     /locus_tag="ACOIAF_001384"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HGZ2668400.1"
                     /translation="MKVFDLTHVTHNDMPVYAEPNRPDIKKVAIIEENGYQETLISVF
                     SHNGTHMDSPRHMYTKGETLDKLDIENFVGKAYVLELEKGNENIELEYLKKYEDEIKN
                     SDFIIFKSGWSKFWDKKQYYVGYPTLTKEAANYIANTNIKGIGIDMLSVDRYDTSVFE
                     VHHILFEKGKIIIENLTNLENVPEKFLFIAAPFKYNDADGAPVRAIAIVE"
     gene            complement(24041..25120)
                     /locus_tag="ACOIAF_001385"
     CDS             complement(24041..25120)
                     /locus_tag="ACOIAF_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HGZ2668401.1"
                     /translation="MKIAIFLGAGASAAENLPIQNELFSEYFKKLNPTNYNTKMNREL
                     YIFFKQMFNIDIVKDNIDNANFPTFEEVLGLLDLAEQRKEAFRNFGLENLNNRSDSIR
                     FLRQYLILLMAEAIHNTPRTDNKYHKLLVENLLENDLLLDTTFISANYDIHIDNTVTG
                     LRDKNDLPIMLDYGVEFTNFKEELNWRKPKEPLIKLYKIHGSLNWLYCPICNSVTLTP
                     HEGGVMKLIENSSETKCLECGELTEPIIVPPTYFKNMSNIFLSNVWNETEKTLRDTDL
                     LIFCGYSFPEADMHIKYMLKRVQTNRKKPPLKIMVFNNHSQKQRITLKKEEGRYKRFL
                     GEDVIFTDNSFQDFSVNPLRFIKNI"
     gene            complement(25253..26599)
                     /gene="pepV"
                     /locus_tag="ACOIAF_001386"
     CDS             complement(25253..26599)
                     /gene="pepV"
                     /locus_tag="ACOIAF_001386"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HGZ2668402.1"
                     /translation="MKEQIKEKVNSLQDEMISSIQESVKIPSVISEATENCPFGENVD
                     KALRGILDLCKSLGFKTVYKDGYYGYAEIGQGEKMIGILGHVDVVPEGDLESWNYPPF
                     EAVLEDGKLYGRGTQDDKGPTISAIYAVKALMDLNVDFNKRIRFIFGADEENLWRCIN
                     KYKENNEEIPNYGFTPDSRFPITNAEKGLLQVHLTCDSKSDIELSVGKALNAVPGKAI
                     YIGKYSDKLKKELDKLNFEYTVEGNKICIIGKSVHSAASDTGINAVARLCIALNNIGI
                     DSNIIKFLAEVIGEDANGNNIIPNCKDDVSGKLTVNIGRVTIDNEKEFAGIDVRIPVT
                     YKKDDFVKELKKMTDKYNLNYEEYDFLDSIYVPEDTLLVKTLRKVYEEETGLDGTPLS
                     SGGATYARALDNCVAFGAIFPGKPETEHQANEYLIVEDIIKATQIYALSIYELLKI"
     gene            complement(26668..27948)
                     /locus_tag="ACOIAF_001387"
     CDS             complement(26668..27948)
                     /locus_tag="ACOIAF_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HGZ2668403.1"
                     /translation="MLNETKELLKDYYGIDDDTFKLSQEIMEEIKDKFEEIKEIREYN
                     QYKVLKAMQESKLSDMHFNWTTGYGYNDIGREKIEEIYSKVFNTEDALVRPIIVNGTH
                     ALTLCIQGIVRPGDEILSVTGRPYDTLEGVIGIREEKGSLKEYGVTYDDVDFLEDGNL
                     DLEGIKNKINDRTKLVMIQRSKGYSWRKSLSISDIKEAIEVIKSVKPEAIVMVDNCYG
                     EFLDTKEPTDVGADVMAGSLIKNPGGGLALTGGYIAGRKDLIELISYRMTSPGIGKEC
                     GLTFGTTRNVLQGFFLAPYIVSQAVMGAIFCSRAFEKLGYDVLPKYDDLRSDIIQCIR
                     LNNADEVISFCEGIQEAAPVDSYVKPVPWDMPGYESEVIMAAGAFIQGSSIELSADAP
                     IRPPYNVYFQGGLTFDHSKMGTLKAIEFIKKLKK"
     gene            complement(28075..28335)
                     /gene="hfq"
                     /locus_tag="ACOIAF_001388"
     CDS             complement(28075..28335)
                     /gene="hfq"
                     /locus_tag="ACOIAF_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HGZ2668404.1"
                     /translation="MKNTVLNLQDLFLNNARKERIPVTIYLVNGVQVKGLVKGFDSYI
                     ILIEGDNRQQNMIYKHAVSTIQPGKYINLTNQNQNNNNNNNR"
     gene            complement(28449..29384)
                     /gene="miaA"
                     /locus_tag="ACOIAF_001389"
     CDS             complement(28449..29384)
                     /gene="miaA"
                     /locus_tag="ACOIAF_001389"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HGZ2668405.1"
                     /translation="MKKIPLIILTGPTAVGKTDLSIKLAKDMNAEIISADSMQIYEYM
                     DIGSAKVTEEEMQGVKHYLIDEVKPDYPFSVSEFQQRAKKYIHEINEKEKCVLVTGGT
                     GLYLNSLIYNMDFAQSDANNELREELQKQLAEKGIDYMHNKLKELDEESANRIHKNNT
                     KRVIRALEVCLSGKKMNDFSSDLKFNEEYQPIIIVLNRDREHLYQRINMRVDIMIKNG
                     LVEEAKKLLSMGFKKDMISMQGIGYKEILKYLDGEYTYEEAIEIIKRDSRRYAKRQIT
                     WFKRYKTAKWFDLDQYENIDELKNEIILYIKDSIK"
     gene            complement(29422..31389)
                     /gene="mutL"
                     /locus_tag="ACOIAF_001390"
     CDS             complement(29422..31389)
                     /gene="mutL"
                     /locus_tag="ACOIAF_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HGZ2668406.1"
                     /translation="MKNIINILDDLTINKIAAGEVVERPSSVVKELIENSIDAGANKI
                     SIDIIDGGKSLIKITDNGIGIPSSEVEKSFLRHATSKIKKIDDLYDLYSLGFRGEALA
                     SISAVSKLEMTTKTKDEIIGTKIYVEGGKIISKEPIGSTNGTTIIIKDIFFNTPARQK
                     FLKSTHAETINISDLINKLAIGNPNIQFKYTNNNKQMLNTPGDGKLVNTIRSIYGKEI
                     TENIIDVEFKCNHFKMNGYIGNNNIYRSNKNLQHIYINKRFVKSKIIIDAITESYKSI
                     IPIGKHAVCFLNIEVDPSCIDVNIHPNKLEIKFEKEQEVYIELRDFLKVKLIHSNLIG
                     KYATYSDKKTQPRIAINSREKSTDYKLRNNDLLESTPKNSNITKGKDEVIEVVTLSSE
                     KPINEFQSVSEVLNASVEDDVKNINYLSEDSANDNIQEEFQVDGIKNEGNYYLGDSIK
                     DSEEEYLCSSKRKFSLYGYFVIGVVFNTYIILSKDDSMYLLDQHAAHERILYERYMEK
                     FYRQDINMQILLDPVVIEVSNIDMLQIENNLELFMKFGFELEIFGNNHIMVRCVPTIF
                     GVPETEKFILQIIDNIEEITSNYDLKGERFASMACRSAIKANDKIYDIEIKSLLEQLE
                     KCENPFTCPHGRPIMVEISKTEIEKMFKRIM"
     gene            complement(31400..34243)
                     /gene="mutS"
                     /locus_tag="ACOIAF_001391"
     CDS             complement(31400..34243)
                     /gene="mutS"
                     /locus_tag="ACOIAF_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HGZ2668407.1"
                     /translation="MQIDMNKLTPMMKQYLEVKNRYKDCILFFRLGDFYEMFFEDALV
                     ASKALEIALTGKACGLEERAPMCGVPFHSANSYISKLVENGYKVAIGEQMEDPSTAKG
                     IVRREVIRVITPGTVLDGNLLENKKNNYLLSLYKDGTNIGLTYVDISTGETNATCLNE
                     DKVIEEIAKIHPTEIIINDLDFIEKLRDIATVSNIYINESFSDNYLDINILKEYFPDV
                     YLQKLKFDDKGLIKSSLSILLNYIYNTQKQITSNINNINIYNSSEYMVLDMFTRTNLE
                     LTQTIRGNKKKGSLLHVLDKTSTAMGGRLLRKYVEEPLINKSKIENRLDVIEEIKDDF
                     ILREDLNDILKNIYDIERICGKIAFERVTPKELIHLKNSIEKLPNLKDTINLSNAKIL
                     KEYVSEMDKLDDIYNLIDEAILEEPTITIKDGNIIKSDFSDELKELREISKNGAFLVK
                     EIENREREKTGVKSLKIGFNKVFGYYIEITKANFKQAKLDETYIRKQTLSNAERYITP
                     ELKEIEEKILHAEEKIKSLEYEIFVEIRDTIYKNIDRIQKVAKTIANIDVFVSLATVA
                     HINNYVKPAINENNKLDIRNGRHPVVENIVGEENFVPNDTYLNRGENIINIITGPNMS
                     GKSTYMRQTAIIALMAHIGSFVPAESADIPILDRIFTRVGASDDLSQGQSTFMVEMNE
                     VSLILKNATERSLVILDEIGRGTSTYDGISLAWSIVEYIQKNIRCKTLFATHYHELTD
                     LEEEFKEVKNYSIAVKEDGEGIIFLRKIIPQGADKSYGIYVAKLAKLPDEVIERAKYI
                     LKDLEKNHVYNSVAINGDKENNNIINSNLDEELVKVNQSNFKNKYESLKIEHELIVKD
                     YKHIKKDYDKLNSKFKALNDEVALMKQNDDKEKINQEDSVKEVALTQISFDSVNRDIL
                     SEEILNLDILNMTPLDAINSLYNLQRKAKEIKS"
     gene            complement(34404..35138)
                     /locus_tag="ACOIAF_001392"
     CDS             complement(34404..35138)
                     /locus_tag="ACOIAF_001392"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2668408.1"
                     /translation="MLTQMNFYHNKKVKVSIENVSKTYKDIEVLKDISIDVYEGEFVS
                     ILGPSGCGKSTIFNIITKLTDYDSGKVNINGKYSYMYQKDLLLPYKTIIDNVSLPLIL
                     KKEKKSKARSMVKPYFEVFGLSGYEDKYPSELSGGMRQRANFLRTFINSNDIMLLDEP
                     FGALDSLTKTSMQKWLLDVKKKVNSTILLITHDIDEAIMLSNRIYVISKKPSIVKKEF
                     VIDSRKLNEDNLENIIKLKKEIISLL"
     gene            complement(35107..36120)
                     /locus_tag="ACOIAF_001393"
     CDS             complement(35107..36120)
                     /locus_tag="ACOIAF_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGZ2668409.1"
                     /translation="MNFKKLGILGLVSVFTLATITGCSSNNNKDKNNDKDTSSKKVTM
                     VLDWTPNTNHTGLFVALDKGYYKEEGLDVEIVQPPESGAETLVATGKADFGISYQEQV
                     TYAKTSEDPLPIKAVATVIQHNTSGFASPKEKNITTAKDFEGKTYGGWGSPSEEAVFK
                     AVMKKNGADFNKLKIVNTGQDDFFAAMKTVDFAWIFEGWDAVKADLIGYDLNFIPVKD
                     LDERLDYYTPLIISNETVLKDNPELAKKFLKATTKGYEYAIKNPEESAKILVKHAPEV
                     DEKLALKSQEYLASKYKDDAPRWGEMKDSVWNNYTSFLKEYKLIDKDMKASDAYTNEF
                     LPQ"
     gene            complement(36142..36912)
                     /locus_tag="ACOIAF_001394"
     CDS             complement(36142..36912)
                     /locus_tag="ACOIAF_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2668410.1"
                     /translation="MKNLEIIDKVKDSIYPIATFLFILILWQSMVGVLEVPQYILPTP
                     VDIINVFFKDYQNLSMHAVVTIGEAILGFIVAIIISLVIGILMDFVSIIKKCLYPIML
                     VTQMIPTIIIAPLFMIWFGFGTMPKVLMVMLTCFFPILISFVDGIENIDKDYLNLFKT
                     MDSNKVNTFIHLKFPMAMDKFFSGLKISATYAVMAATVAEWLGGTKGLGVYMLRSKSA
                     YALDKVFASTILVVIFSLMFVGIVQAVKKVVIRHRLID"
     gene            complement(36887..37177)
                     /locus_tag="ACOIAF_001395"
     CDS             complement(36887..37177)
                     /locus_tag="ACOIAF_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HGZ2668411.1"
                     /translation="MVIADIAVMPLRPYKGEEEMYKVVDACIAEIEKSGLKCEIGAMS
                     TTVEGDFDEVFELLKKVHKIPFNLGCERVITVARVDEKAGGLTIDEKLRNHR"
     regulatory      complement(37308..37407)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
BASE COUNT        12655 a         5872 c         4338 g        14586 t
ORIGIN      
        1 gtaaaaattt atttaaaaat acaactatct acataaatta tatttatata attatatgtt
       61 atcaagctgt ctgttgtatt taataaatcc ttttgataac atataaaagt aagataaatt
      121 tttccattaa ataccttata attatacaca aaatctctat atacgttaaa cagtatagag
      181 aaaattttca tagaaccatc tttatagggg atactttata aaagaataaa aaaattataa
      241 tatataaaaa acttcatagt agtgtaacaa catcagtaaa aatatgaaaa cactatataa
      301 aaattcaaaa atattaagaa taatgaaata cttgaaattg cttatatcaa gaaattttat
      361 ataatataaa ctggtcaaat atgttataat attaaattgt gacaagttca tggaggtgtt
      421 tatatgaaaa aacaaaagtc gccaatttta cttgaaatgc ttggactatt ttttggttgt
      481 atatcaatga gtattggtat aaatatgttt ttaaaaccac atacaattgc tcctggagga
      541 cttagtggtc tatcattagt actaaacaag gttacaggac ttccagtatc agctatcatg
      601 ttaattatag gtgtcccatt agtaatactt gcttttagaa ttatgggtac aaaaaattca
      661 ttaaaaactt tatttggaac agttgtattt tcagctatag ttcaattaac agacccatta
      721 tcaaaactaa gatttactaa tgacttatta ttgtcttcta ttgcaggagg aatcctagtt
      781 ggtataggtt taggaataat gtttaaatca gatgcttcta ctggaggaac agacttaatt
      841 gcactcatac ttagtaagaa attcccagga ataaaagcta caaaattcat gtcatgccta
      901 gatggaatgg ttgttatatc ttctggtata gtaaatcgta gtttagaaac tgctctttac
      961 tctggtatag cactttgtgt attaataaaa atagcagata tgataatgga aggatttgac
     1021 cattcaaaag cattttttat tatctctgat gagcctgaaa gtttaagaca agctataact
     1081 gaagaacttg atagaggtct tactattctt gatggtaaag gtggatacac tagagaaaat
     1141 aaagaagttc tattagtcgt agtttctaaa aaacaagaac tttatctaaa gagattagtt
     1201 aaaagaactg accctactgc atttgttatt gttacagatg tacatgaggt tcttggagaa
     1261 ggatttaaaa atttagagaa ctaataaaaa ttaatatttt aagtatatat actattttaa
     1321 ttaaaatacg atttattcaa aaagaatgtc tcataaactt tttagttcat gagacactct
     1381 tttttgtata aaatcaaatg cattttcagt attttaacaa taaaaaacag gattatctat
     1441 attttgaggc aaccacttta ttgaaatttt atttagaaaa caatcattac taaataacca
     1501 gcatagatat taaatgatta tatctattgt tttttcaatt catattgata acaaggaaca
     1561 ttttttacag tatttccagt gtgaattttt ttgatatact ttcctgtaca tttaaatcct
     1621 aataaatcta atgattttgt agaattttgg ttttcttcat aggttattgc aattatttta
     1681 tttaagttta gttctctaaa tccatactgt attactcttt ttgttatctc agtagcaaaa
     1741 tcatgtcccc aatagcttgg atttatacat atacctattt ctgcttctaa tgctttagga
     1801 tttatattat taaaccccat atttccaata actttattgt ctcttttata tactatagca
     1861 aagtggccaa ttttcccttt tttatatgga tttagaaaaa atgattttat aaactttctt
     1921 gtatcttcta aagatttatg ctcactaaat ggtaaatgtt ttactactat tttattttta
     1981 taacagtcaa aaaaatcttc aacatcatct aatgatgcag gtcttagtat acaacttcta
     2041 gtttctaagg ttttcatttg aggatattca gaatcagaca tttctaatcc ccctttatac
     2101 attaatctaa atatactgtt catatttaaa atacgatatt aattatataa atgttctcaa
     2161 attaacctta tttttatttt taataaaaac taattactat atgaatcttt caagtttacg
     2221 aatttatctc tatttttata ttaaattact tgtgaaatat gtattctaaa ttatatatta
     2281 ttagatatca tgcttaaaaa ttctttggtt gctaaggata aagatatatc ttttaaatgg
     2341 cagtaaccta tatttctctt aggtattggc tcttttatat ctaatttaaa caatttctca
     2401 ttttccaaat aatctatgct aaattcttct ataacacaag acactccaag atttatatac
     2461 gcaaattcta aaagtaattc atgagaaccc aattctatat ccgggtttag ttttatacct
     2521 tttgataaaa aatattcatt tacataaagt cttgaatttg ctttattttc tagcattatt
     2581 aaaggcaatg tgtttaattc ttccaatgat attttcctac ctttgtattc tatgtaatca
     2641 tttgcacata caaatatatc atgtatttca atacactcaa caatatttag tgtatcatct
     2701 tctattggca tgtttataat tgctatatct atattcccat ctttgagtaa agatataagt
     2761 tctctactcg ttctatttat tgtagataca tgtatatgtg gatacttctt atgaaaaatt
     2821 tctaaatatt ttaacaagaa aaaacgtgca gctgtatctc ctactcctat tttcagagaa
     2881 ccgtattcta attttttaaa tttatttatt ctatcttctc ctgatgatat taactttaat
     2941 gcagtagtga catgttcact tagaattttc ccctctggag ttaattttac tcccttattg
     3001 tttctataaa ataataaagt atctaattgt tcttctaatt gctttattga ctggcttaca
     3061 gctggctgtg acataaacaa ttccttagca gcaagagaaa aactcttatt atttacaact
     3121 gcattaaaaa ccttataaag ttcaagtttt atattcatat ataattactc cttatgtcaa
     3181 atattagtat tatttatttt acttataatt atatttgaat tataatatac atggtactaa
     3241 tatgacaata ttaattcttt aggaggattt tagataatgg atagattagt tggaactgtt
     3301 tcaagagggg ttagagcacc tattataaga caaggagatg acttagttaa aatagttgtt
     3361 gattctgttt taaatgcgtc aaaaagtgaa aactttgaag ttagggacaa agatgtaatt
     3421 gccgttacag aagctgttgt agctagagca cagggaaatt atgctcacgt tgacaatatt
     3481 gcaaaagatg taaaagataa atttggagat gatactgttg gagttatatt cccaatttta
     3541 agtagaaatc gttttgctat atgtttaaaa ggaattgcaa aaggatgtag aaaagtcgta
     3601 ttaatgttaa gctacccttc tgatgaagtt ggaaatcact taataagtat tgatgaactt
     3661 gatgataagg gaataaatcc ttggtcagat gttttaacag aagaaaaata cagagaacta
     3721 tttggatata acaagcatac atttactggt gtagattatg ttgattatta taagaattta
     3781 atcaaggatt gtggtgcaga ggtagaaata gtatttgcaa acaatccaaa aactattctt
     3841 aactatacaa caagtatttt aacttgtgat atacatacaa gacaaagaac aaagagaatt
     3901 ttaagacaaa atggagctaa aaaagtatat tcacttgatg atataatgac agcaagtgta
     3961 gatggtagtg gttacaatga ccagtatgga cttttaggtt caaacaaagc tacagaagaa
     4021 acagtaaaat tattccctat aaattgtgat gaagttgtaa acaaaatcca aggtaatata
     4081 aaagaaataa cagggaaaga tgtagaagta atggtttatg gagatggagc ttttaaagac
     4141 cctgtaggta aaatatggga attagctgac ccagtagtat ctcctgctta tacaaaaggg
     4201 ctagagggaa ctccaaacga agtaaaatta aaatacttag cagataatga ttttgctgac
     4261 ttatctggtg atgagttaaa agaagcaatt tcaaaataca tagttgaaaa agataataaa
     4321 tctgatgatt taacaggaaa catggtttct caaggtacta ctccaagaag attaacagac
     4381 cttataggtt ctttagcaga tttaacatct ggaagtggag ataagggtac tcctattatt
     4441 tacatacaag gatatttcga taactataca aaatagtatg ttttaataag atttaatttt
     4501 tttggattat atatattaac atgaaaattt attaaaacct cttaatagat atgacttttc
     4561 aagagttata tttattaaga ggtttattta atgttcacag ataagatata tttaaataat
     4621 ttaaacaaga ttgtcagaaa gttatataat cttaaaaatt tatataactc taattcttat
     4681 ttatttctga aataatatca ctcttcaaaa tttgtcttac tggaataatt acagcaacta
     4741 tactaatagt gatatttaaa ataatcatac ctatgtatag aggtgtattt attttaagaa
     4801 ttggatttat atataaatat acacgtttta ccataaaata aatcataaat tgtccaataa
     4861 cacttccaat taccattata atacttgcat agaatccata taaaaaacct tctcttatca
     4921 tcataagtaa aaacttatta tccgtaatac ccatagctct taaaattcca aactcatgtc
     4981 ttcttgaaaa aattaagtag cttactgtat tgattatatg gaacaaactt attgcaagaa
     5041 gtacaaatac aataccatat ataaataaga gtttttgatt taagtaggta ttattttttt
     5101 caatagcaat ggtataatct gttacgatac atctttttac atcacttaca acacttttta
     5161 tattctcaga caattcctca ctgttactat cttctgtctt ttttatactt actgaattat
     5221 aattttcgat atcaaagtta tttttcattt gcccattcgt cataactata tccataccat
     5281 aatcaccaat gaaatatatg ttatttgcca tcacacgttt tacagtagca gcaactacaa
     5341 attcttttgt tgtataatca ttcttatttt cttctttaaa tttaacaatt tcatctgttg
     5401 gtttcattgt ctttggcact ttaactttta ttgtatctcc tggatttata tcaactgcat
     5461 catacatccc agttccatcc ataggtagtc taactataac cttattattt ttaaccatat
     5521 catgggtatt aatctcccca tctataatat aagaatttaa tccttctaac ataccattgt
     5581 tataaccata aatatttgtt tttaaaagat agccaccttc attttgcttt gtacaaatac
     5641 catcaaaaaa agtctttatc ctgtaatctt tttcaagagg tttaaagaaa tttttccaaa
     5701 atagttgttc ttctttaatt tgcatagctc caaataaata tcttactgga tagacttttg
     5761 atattccctc aacattgttt aatcttgaaa ttttatcttt tggtatacct acatcaaaat
     5821 ctgttgtttg catagttatt tggtagtcag aatttaaacc atcatcagct ttcattgtaa
     5881 gttcattatt atttttagta agctgtatag agtaactaga acataaaaat ataattccac
     5941 caagtgataa tgaaaacaaa attccaataa acattccctt tttttgagtc atgtatttat
     6001 gagataatac ttttgtcatg ttagagtgtt ttacactttc taatattctc tttttcttat
     6061 tagtaatctt aaagtttttc atagattcta tactagagta tttattcaac tgatgtacaa
     6121 cacgtacagc aattaaaatt aacaacaata agataaataa aaagcctaaa taaatagatt
     6181 tttcacttat aaaaaatgct cctggaacaa tatcactact caaaaatata tgtggaaatt
     6241 gttcatatat tgtagacgct actccaatac ctaagaagca acctatagga aatccaacaa
     6301 taaaaagtaa aaataactct aaaaatagta gaaataaaag ttgtttatta ttagcgccta
     6361 atacttctaa aaccccatat tgtgatattc tctgaagtat tgaaacatgg aaaatactat
     6421 atactgctgc tgcaccaaac actccaacaa gcaatataat tgcatttgta tttagttcta
     6481 aattttttaa gaactcaaat atattatatt tttctatatt ttttggtggt tttactccaa
     6541 attcaactga aagaggccag tttatataag ttttttcttt tgatatattg aattttttca
     6601 taaatttata attttgcttt ttaatatttt tattttcatc atattttaca taaactatat
     6661 aagaatcctt ttctttaggc tgaaaataat cgtttacaaa tccctccaga gcagtttctg
     6721 ggtttgaaag acttgataaa ataccacata acttatagat tttaccatct aaattgattt
     6781 ctgttccaat ttctttgttt acatttaaat tgtttagaga atatgtgtca agtactatct
     6841 ctccatcttt actaggaaga gttcctttta ataattttct acctgtcatg tctaaatatg
     6901 aagaatcagc atttaaaaat tctataataa aaggttcatc tatctgattt ttaggttttg
     6961 taatgccaag tctatctaca gtgtattcct ttgatttttt attttcttta attttattaa
     7021 gttgttcctt atctacttta tagaaataat gataatctcc actatcttcc cttatctttt
     7081 caattctact tttgtctgca ctatgaacta aagaaccaat tcccgtcaat aatgctactg
     7141 atagtactat gcttaaaact atagatatag attgtttttt ataatgcttt atatattgaa
     7201 ttgaaaattt tactagagac atcatagtac ttcaccacca gtattcacca caactccatc
     7261 ttcaatatgg ataattgagt cgcatacttg tgctatattt tcattgtgtg ttaccataag
     7321 tatcgtttga tggaattttg cactactttc ttttaataaa catactactt ccattgtagt
     7381 tttactatcg agatttcctg ttggttcatc tgctaatata agcgcaggtt tatttgccaa
     7441 agctctagca attgctaccc tctgttgttg accaccagat agttcgcttg gaaattttaa
     7501 acgcttatcc caaattccca atgtcttcat cagcatttca atatattcat ggtctatatg
     7561 gtttccttta tctaatgtaa caggaagagc tatattatca taagtgttta atacgggcat
     7621 taaactgtag ttttgaaata taaatcctat atttcttctt ctaaatactg tcaaatcttt
     7681 ttttcctatt ttagatatgt tctttccttt aattattaca tcacctgaag ttggattatc
     7741 taatccacct ataatattta acaatgtgct ttttccagaa ccagaagttc caactatagc
     7801 agtaaatgta tctcgttcaa tttctatact tactccattt aaggctttta ctttagtctc
     7861 accttttcca tagtgtttta ctaaattatt cgtcactaat atactcattt aatttctcct
     7921 ttcaatacag atatatgttc tataaaatat tgtaagtgac gataattaca ctcatcctac
     7981 aaaattagtt taaactctta caattctgta agaagatact aaaacaacat ccacttccta
     8041 gtttagattc tactataatg tttccatttt ctttttctaa gattaatctt gtaagatata
     8101 gaccaatccc tgtgccattt tggttttcca catttttact tctgtaaaat cttttaaaaa
     8161 tatcatttag ttctctacta tcaataccta ttccctcatc tttaatagta atcttcgtat
     8221 atagttccat ttttattaca gaaatagtaa tcgtgctatc tgatggtgag tattttattg
     8281 cattttctaa aacatttaca attgcttcta tggtccactt tttattatga actaacttta
     8341 tgtctgaaaa tggttcgaga tttatcctta tatttttagc ttctgcttta ttaaagatag
     8401 tactgatact ttgaataaga gtatctttaa ttagtagttc ttctacttca aattctataa
     8461 ctccatcctc taatttagtc attttaaata aggattgtaa caaccagtca atcttaaaag
     8521 tttcattttt catcttttgt aaaaactcct tcttctgtaa tgttgaaata tttatattct
     8581 ccagaagttc acaataaatg gttatatttg aaagtggagt ttttaattga tgtgacatat
     8641 ttgatattaa acctttaatc gcttcttttt ctgactttga ctgttctacc tctatttcta
     8701 tcatatcctt aatttttttt acttggtgag aaatttttga cgctcttgta tccttcgtat
     8761 ctggataagt aacttcttta ccttccaata tttgatttaa catcaaattt aattcatcat
     8821 atcttctttg atttatttta gtagatacta taaatataac aactgtacat attataaagg
     8881 ctaatataaa catattattc tacctcattc cataaataac ccattccata agctgttttt
     8941 atatattttg gattagatgg gtcatcctct acttttactc taagtcttct tatatttaca
     9001 gatacaatat tatcatcaac ataattttct tcgctgtccc aaatatggtg taaaatttgt
     9061 tcctttaata atatttgacc tttattttca attagataac ttagaagctt atattctaca
     9121 gagctaagtt ccaaacattc tttattttta tatactttta gtaacttttg gtccaatttt
     9181 attccattgg aatatattac tttattattc gatttttttc ttattgctac tttgactctt
     9241 gcctttaata cagataaaga aaatggtttt gtaatataat catcaagccc tatttctaat
     9301 ccttttactt catcaatttc tgagtctctt gcagtaagca ttataattgg tatatctgac
     9361 ttttccctta ttatttgaca tacatcaaat ccatctccat ctggaagatt gcagtctaag
     9421 attataaaat ctatattttt actattaaat atatttaatc cttcttgttt tgtataagcc
     9481 ttaaatacat taaatccatc attctcaaat gcataaaaaa gaccttcatt taagtttata
     9541 tcatcttcaa taattaataa gttcaatttt actctccttt agatttttta gataaataat
     9601 tattttcttg cattatcata ctaaaaatat tttaatttta tttatttcac taaaaagatt
     9661 gtatttaaat ttgttttcaa aataaaaaca tcataatcaa gcatatagtt atatatttcc
     9721 caatttaatt acgcttaaat tgaaaatata actttattct tatataatta aattatacaa
     9781 taataaagtt atatttttta aatatatttt tactaggggg aatagaattg aaaagggttg
     9841 atgagagaaa tactatgttt gcaagagcaa attacaaaaa agatagtact gcttataatg
     9901 actattataa gaaaaatcct gacaaaaaag aaattgatga tagtataaga aatagaccaa
     9961 atttatgtag tgaaggaaca atgacttaca atgagttaaa ctctccaatg gcaagtagtg
    10021 cattcgattt tttatctgat atcaaatctt tatgcgaagg aaaagtttct gatactaaag
    10081 tagatgtaga tgcaaagtca atgacaaaaa aaattaaggg tcttgcaaag cagtatggag
    10141 cttctgtagt tggtataact aaattaaaag actatcatat ttatacaaat aggggtagac
    10201 atgaagaaaa ttacggtgaa gaaattaatc taactcataa gtatgcaatt gtatttggat
    10261 gtgagatgga taaggaaatg ataaatagag ctccaatgat atgtgaagtt atagaaacat
    10321 caaagtgcta tgtggatgca tctatcgtcg gcatgatttt aagttactat ataagaaatt
    10381 taggatatga tgctagaaat catatggatg ctaattattt ggtaatgcct gtttttatag
    10441 ctcgtgatgc gggtttagga gatataggaa gaaatgctat acttacaaat aaagattatg
    10501 gctctagact tagattaggt gttgttacta cagatattcc tcttttagaa gatgaatatg
    10561 ttgattttgg actagaagat ttttgtaagg tctgtaaaaa atgctctttt aactgccctt
    10621 ctcattcgct ttctaatgat attaaaattg gtgatgatgg aaaatataat tgggtaattg
    10681 aacatgagac atgctatatt aaatggagat atttaggtac agattgtggt atgtgtatat
    10741 ctgcttgccc atttagccaa aatctcgaaa ctataaagaa tactacttca tttaagggta
    10801 ataatgagct tattcaaaaa gctttagatg agtatacttc aaaatttggt aaaagaatat
    10861 ttatacctgg aaatccatct tggttgaaat aattttattt aataaagaat ctgtactaga
    10921 atttatcata ggacaggttc tttattaaat atataaattt ttaaatactt tttctcgttt
    10981 ataacaattc agcaatgttt ttagtctata attgacatta taaaatatta aactaaaagg
    11041 agtaactgta tggaaaaaat attagtagta gaagatgatt ctatattaaa taaaactcta
    11101 tcatataacc taattgaaga tggatatata attacttcaa aatttacagc taaatctgca
    11161 ctaaagtcta tttttgaatg tgaatttgat ttaataattt tggatattaa tttaccagat
    11221 aaaagtgggt ttgaattatg taatgaaata aaggaaaatt acaatatacc aataattttt
    11281 ttaactgcta atgatatgga atgtgatatg attaaaggct atgaacttgg agctttggat
    11341 tatataacaa aaccatttaa tatcaatata tttaaacaga aagttaaagc ttttttaaat
    11401 catcttacaa taaaaacaaa acaagattac tatagggatg gatatcttga aattaacttt
    11461 tctgagttat ctgccaatat aaatggaaat caaattatat ttacaccatt agaatatcgt
    11521 actttaaaac tgcttactga aaatcctaaa agtattttaa caaggaaagt acttttagaa
    11581 aaactctggg atatagatgc taactttgta gatgaacata ctttaacatc tgttataagt
    11641 aggattagaa gcaaaattga aaaagacaat cttaagtata tcaaaacggt atatggtatt
    11701 ggatatatgt ggttaggtga aagaaatgaa ttttaaaaaa ttttctttta gttctattta
    11761 tttaataatt gctgttggta taatttttac agctatttta tttagtatct caatttatct
    11821 aataactgat gatttaaaac taattgctct attattaata tatacaatag tactttgtgg
    11881 atttatagca ttaacaatat ttttacttca taaaaaaata gttatatttt gttctaaaat
    11941 atgtcacact ttagatagta tgatgaatag tgaagataat attgaaattg aattagaaga
    12001 agaaactatt ttatcacgta tcaatcacag attagttaga atgtatgaag ttattcaaga
    12061 aaatcgcaat agtattgcta tagagaaagc tgatttacaa gaactagttt ctgatatatc
    12121 tcatcaaatt aaaactccta ttgcaaattt aaaaatgatt aatactactc tactaaatca
    12181 aacatttaaa acagaaattc aaaaggattt tttaatggat atggaaaacc aattagataa
    12241 attagatttt ttaatgcaat ctatggtaaa aacctccaga ctagagacag gtataatcac
    12301 actatctaaa aagaaaaatt caatttatga aacaattgct acagcactta gtggaatttt
    12361 gtttgatgct gaaaaaaaga atattgaagt tacagttgat tgtgacccaa atttatatat
    12421 aattcatgac aagaaatgga cttctgaagc tatttttaat atattagata atgctgtaaa
    12481 atacacaaat tctaatggta agatacgtgt agctacagaa tgttgggtta tgtatacaaa
    12541 aatagatatt attgataatg gaaaaggaat atcagaaagt caccaagcag aaatctttaa
    12601 aagattttat cgtgaagaag atgtccatga catagaaggt attggaatag gtctctatct
    12661 ttcaagaaaa attatatctc ttcaaggtgg ctatattaaa gttacatcag atattggaaa
    12721 agggtcgact ttttctattt tcttacctaa taaataaatt tttaaatatt tcatttgtaa
    12781 tgattctgca acattttggt tttatactgt atttacaaga tgttaaggag gtattattat
    12841 gagtatttta caagtaatta atttaaagaa atattatgga caaagttcaa acattacaaa
    12901 agctcttgat ggtgtaactc tatctattga aaaaggcgaa tttatttcta ttgtaggtac
    12961 atctggtagt ggcaaatcca cattattaaa tatgatggga ggattagata atcctacatc
    13021 tggaaaggtt attataaagg gtaaggaatt atctactatg actgatgaac agcttacaat
    13081 ctttagacgc agaaatatag gttttatatt tcaaaattat aatcttgttc ctttacttaa
    13141 tgtatatgaa aatattatct taccaatagg attggatgga gagagcatag ataaggattt
    13201 tataaatgaa attataaata tgctttctct tgaagaaaaa ttatatgata tgccaagtaa
    13261 tctttctggt ggtcaacagc aacgtgttgc tatagctaga gcacttgtaa caaaaccagc
    13321 tattatacta gctgatgaac caacaggtaa ccttgatagt agaactagtt cagatgtact
    13381 gagtttactt aaagtgacaa gtaaaaaatt caaccaaaca attgtaatga ttacacacaa
    13441 taatgaaatt gcacaactaa ctgatagaat tattcgtatt gaagatggaa aaattgttaa
    13501 ctaggaggtg tagtatatgt ttagaaataa taatcgcaca tttataagaa aaatagcttt
    13561 aaatgattta aaaattaata aacttaaaac atatctttca ggtatcatta taatgatttc
    13621 tacatgttta ttattaacag tgaccttagt ttcatataat gcctctgtag atttagttaa
    13681 tgcatctcca tatcatgcta tatataaatc tgtagatgaa aaagcaaaaa atatactata
    13741 taaaaataaa aactttagtg gcgtaggaac ttacaaatta attggaagta ataaaaagac
    13801 agattgtatt atgtcgattg tttatgcaga tgatacagca attaaactta tgaattttca
    13861 accattgaaa ggcaaactac ctacaaataa aaatgaaatt gctatttcag aaaaatattt
    13921 acaggaattt ggtttagata aagatattgg agatagcatt aaattaaatt attatgatga
    13981 tattacgaat aaagaagttc aatgtgattt tattattgtt ggatttctag aaaattatta
    14041 tcaagataat gccaagcaat actatacagt cgtttcaaat gattatttta agaatatagc
    14101 ttctacacct ttacaaaata aaagtaatac ttttgacgag tcaagacctg atactgtaga
    14161 tgtattagtt aaactatcag aagaaaatac agagaaagat tcagcttcaa ttaagacaca
    14221 gttaaagaaa attggtttat ctttaggtat taagaaatat aatatatact taaatgataa
    14281 ctatattgaa tcaaatttaa ttgatggtga gcagattatt acagttttag tagttggaat
    14341 agttgttctt ttttctagcg tttttgttat atatagtatt ttttatattt cagttgttaa
    14401 ttcagttcag atgtatgcta agttgaaatc gttaggtatg acttcttttc agttaaaaaa
    14461 gattatttct ctacaaggaa atattttgtc aattattttt attccattag gggttatagc
    14521 ttcttgtata attgcttaca taattcaacc attagcatgg caaatgatag ctgatttatt
    14581 tataatttta attttatcac tggtaatgtt tttaactgtt agaatttctt tatttaaacc
    14641 agctagaatt atttctaaaa tatctgctat tgaagccatg caatacacag aaactaaatt
    14701 tagaaaaaaa gcaaaaaatt ttagttatat aaatataaaa aatctagcat taaagaatat
    14761 cgagtcaaat agaaagaaaa atttgattgc attaatatcg ctaagcatta gtggagtttt
    14821 atttatttca atcgctaatc ttgctaattc aatagatttc aaaaaacaac tatctcaaca
    14881 atttgtctat aacgaagatt atataattag cattaagagt gataacttat atgagcatat
    14941 tacagaaatc caacaaaata atccactaac agattctctt aagaatgaaa ttaaatctat
    15001 tcatggagta aaaaaattaa ttgaatccaa atcaattaaa agtaatatta tagaacctta
    15061 catcaaagat agagacgaaa aacaatttat tactctaatt aaaggtatta cgcctgaact
    15121 ttcaaaatca cttgaagaat atattgtcag tggaaaaata gactatacac atttagattc
    15181 agatagttta attataaata aatatagaac aaaatattat ggattaaatt taaaagttgg
    15241 agatacagtt acatttaata tctttagtgg aaatgatatt attaaaaaga aaatgaaaat
    15301 tataggaatc attgacaatt ccaatacagg aggtatgttt tttgcttcag acaaaacttt
    15361 agaatctcta acaccatata atacaaattt agatttttct attttaatag ataaaaagca
    15421 ttctaaagaa gttgaagaaa agttgttaag tataattcag aaaaattcaa atttaatgct
    15481 ttattcatat gaagatgatt atagaatgat tactagagca tttcaatata taattatagc
    15541 ttcatacatt tttgttggtt taatttcttg ctttggtata ctaaatatga ccaataccct
    15601 aataaacagc gttttaatta gaaaaaaaga gtttgcatta cttcaagccg ttggaatgac
    15661 aagaaagcaa ctgcgaaata tgctttatag agaaggtcta aacataagta tcaaagctat
    15721 atgtacttca agtatactag gttattttgg ttcaaatcta ctttgcactt ttataaaaga
    15781 tgtaattaga ttagacttta ttaattttaa attttctata tttactattt taattttttc
    15841 attcgtatta attggtatac aagttttagt gactgaatta cttgtaagaa atatagaaaa
    15901 aaaatcactt acagaacgct tacgttctca gtaagaatgt atataaaaaa tagccatttt
    15961 ttatacaagt tttaatatga ttttttaaaa gctataggga tagaaaattt tgactttaca
    16021 taattttcta tccctatact tgtttttatt ttatacagat acacttcctt atatctccga
    16081 gcaacatcac cagagacata aacaagtata ttccttattt tctaaatatg tcaattgcat
    16141 gacttgtaac ccctactaaa tcttcacgtt cacaagtagc aaggtgatat tttaaatata
    16201 atgcaaatgc atcatcatcc atagagtcta ctgcttcacg catatataaa gccaaaccat
    16261 ctgatgcgac ataatgcagt ctagttacat taaatgcaga catcaaatca tcaatgtttt
    16321 ctttacgtac aagctcaaac aaatcctttg gttcagactt agcagcaaat gtctgtggat
    16381 caatcaaacc tttttcaata tattcagata cattaatatt tcctctatgg aacccctcgt
    16441 caatgagaca gccatcagat ataacatatg cagcaaaaac cacaccacca ggttttgtta
    16501 cacgaattgc ctcatgtaaa gcttgttgtt tatcttcttt gttataaaga tgatatagtg
    16561 gtcctaacag taacgtaata tcatatttat tatctgggaa aacagataaa tccatagcat
    16621 taccctgagt aatgcttata ttttcatttg attgagtatt tttatggaaa acctctatat
    16681 tatgttcgac taattccact gcgtcaacat catatccctg acgtgccagt gtgtgagaat
    16741 aacgaccagt tgcagcgcca atttcaagca cacaatcacc tgattttatg tacttatcaa
    16801 tgtaatgcat agtagtgaga aattccacca ttccatgttt taatgcaagt cgactatctt
    16861 catcataagt attataaaag tcaattaagt attgttttgt ttccataatt tcacctcttc
    16921 tatattagtt aataaacatc aaatagacaa attccaatct ggtttttctt gtcttataaa
    16981 agctgttaat atatttttca acattttttc agcactatat ctatctaatg ttaatttttt
    17041 tgttgctgtt aagacagcta tgccatgagt ataaagaaat aaatctaaaa atattacttt
    17101 tgcacattcc aactctattc caatgctttc cgaaaaaagt cttgcctttt tttcattgtc
    17161 aatttcttta taaaaatcat ttgcctcttt catcatcaaa tctaaatcat ttataaataa
    17221 caacttaaat aaatgagttt cttcttgagc aaattcaatg tatgaaagcg gaagcagtaa
    17281 ataaggactt acacttttag aattactata attaacaaca tactgattat agtattcata
    17341 tgcaaaagca agaaattcat tttttaattc attcatgttt tcgtaacaag taaaaattgg
    17401 tcgagtggaa cactgtaatt tacttgcaat actcctcgca tttacagctt caaatcccac
    17461 ttctcttgta atatccaaga ctgtattcaa aatcatttct tttgttatct ttactttagg
    17521 tggcatatat gttcacctct ccattaaatt tttataaaac acatatgtta tataactata
    17581 gtaacatatc aagaaattat gtcaagaaaa agctttgcat ttttttgcaa ttcctaaaac
    17641 ttataaataa aacatgagtt gcaattaatc tattaataga taacagcaac tcagattttc
    17701 ctttaaatac tcttcttttt gtctaatatt tcatatacac aatcttctat tgaattaata
    17761 tctttttctt ttcttttttt agaatcaatt aaaatgaaat acagtataaa acctacagca
    17821 agtccccatg ttccgccttt agcagcaatt acacctccca taagtccaat caatcccttc
    17881 tcaacacctg tattacatag ctccattgat agctttgtac ataaatatcc ttgaactaaa
    17941 agtgttactg acaatgctaa tggtataaga ggtgtagcaa tctgcgcaat tggataaaaa
    18001 cagataaaaa ttgctgaaac ccatactaat gtacccatgc cactaaaaac agactgcatg
    18061 gcttcctttc caaccttgta cctttgagca acacttcctg taagtgtcgc agctaatgga
    18121 ccgcacattg gtatatatgg agaaaagatt gccataaaaa tatttcttat cccacttata
    18181 acattagacc tatttgaatt gaaatctata tattcatctt ttctattttc actttcagaa
    18241 agtactaagt tttcacctgt tataaaatca ccaaaggcaa ttatgtatat cataaaagcc
    18301 attggtatcc cttgtataaa taacattaca cttggaaaac ctattccaaa aggagatact
    18361 gtatttatca gatttttaaa atctactatg taaactaaat tgtcaaaact aaatctagga
    18421 atttctattt ctttactaaa tatacctact attaaactca ctaaaatagc tgatactaca
    18481 ccatattctc ctaaatgaaa taaaaattta ttcttaactt ttagagtatc aaatctttca
    18541 gaaaacatta caaaacaagc aaccaaaatt cctactgtta tagatatagg ataagaatca
    18601 aatctccctt taggattaaa ctctgatatt atagtcgata taccgacacc cattagtatt
    18661 ccacctttaa tagaatttgg tatcttacta actaactttc cagctacacc agttataccc
    18721 attaaaataa atattattcc taaatctatt tgtaaagctg ttaatgcttg tattctttca
    18781 atgcccatgc tgaactttaa caaatatgca gttgtaagag ttatagaagc tgtaatccag
    18841 ccaggtacaa ctgggtctcc cataaaaaca tgcaaacagt ataatattga gttaatgata
    18901 gccatactcc atgccacttc atagggtaat cccaaaattt gtgtactaag gccaattgtg
    18961 cctaaacttg tcatacttga tattatacct tgaatagctt ctggtttctc aaatctgtaa
    19021 tgtataaatg gaattcttat cttaaacatt ccaaatttta tatgaggttg ttcccttcta
    19081 gcgagtcttt ccataacttc actccacctt tcaataactc ttataagttt acaactttat
    19141 aattgtttat aaattatcaa tgtcattaaa attagtatat aacatgcaaa ttatataaac
    19201 aataaattaa atctataaaa ttgatagatt ttgtgcatat atagaaagac cgcttcattt
    19261 ttagttactt aaatcactta atattagtta tttaaatcac ttgataaagg ttttttataa
    19321 aattcaatag ttctattcca tgattcaaac tgtccttttg aatttccttc tacagttcca
    19381 cctgaagaaa aaatccctct actcatactc atacataaag tctcctttag aggcataata
    19441 tatggaattg gtaacggatg acccatatta ttgtatgtta ataattctac gttatatgga
    19501 taattatttt cttcaaagat ttctaatgcc tctaaacaag cttcataact attccatatg
    19561 ttatcttctt ttccagcaat cataagtata tctgctttgg aattttctat ttttattcta
    19621 gcctcttttc tattctctgc tcttttaaga cttttcttat aagtactatt aaatccaaat
    19681 ggaatatctt ttgatatatt ttttttctgt tcttcaaaga atatatcatt gtctactttg
    19741 atatatggaa atggctctcc tttatatgac catgatgaag tatcatttcc actcatcaat
    19801 ccattaagag cttggaaaca ataaatgtga ggttgtgata ctacaacctt tttaatttgt
    19861 tcatatcttg aagcaagtaa aaaggccaat tctccaccct ttgatgtccc atgtacaaat
    19921 atttctttag tactggtaat ttcattttca ctaatccact caaatacttt ttcaaaatat
    19981 tctaatggaa cctcttctaa tttatctggt aacccttcta aagcaaagta tgcaactgtt
    20041 agtacattga atcctctaga tgcaagtgga gctgctaaaa ggtctagagc gtctaaattt
    20101 ccatcagaac cacctagcat taaaattgtt ttattattag aattttgttt ataaaataat
    20161 tttcctataa actcatcaga tatgtatttt gtggtaatat cttctgattt aaatattctc
    20221 tttaattcaa cttcttttct ttcaatagaa gtttcaaata tcatattcat aattattggt
    20281 ttatcaatag atatattttc tgcaaaattt ttaccttcag ttttagattt ttccagcgaa
    20341 taaattaacc ccatacaatt atcagcctta taagtacctt ctataggttc aactaaatct
    20401 aaatctactt ctcctttttc atttgaacaa aacactccat aagatgaaaa ttcttctccc
    20461 ttacaccatg gaaattccat tttcacactt attttaagtt tagtatttgg aattaagtct
    20521 gaaaccataa tcttaagtct ttcatccaca aatgattcta cattgtatac atttactttc
    20581 ataatctatt tctccttata ataattattt atgttttata taaaacagct ttatctaata
    20641 caagataaat aaatttttaa attaagacac catattgact atttcatttt atcaacatat
    20701 ttcatctttg tcaacatatt gtctttttta gtaatttaaa attatggtct atatgaaatc
    20761 cagttgattt ttggctaatt aaagatagga atgctattag gtttagaata tatatattca
    20821 aaataaaaaa ccactcattt taagaatggt ttttaatata aactatttgt tattaataag
    20881 gatatttttt tatttaaatt tatataatcg aacatctaat atttaatgtt taataaaatt
    20941 atgcatttgc aaaagtatat atggcattaa ctcttgctac atataaattc tcatcactac
    21001 atccaatata tctagcatat ttttcaatct tttcttttgc attttcaaca tcattttcat
    21061 cgctctccat taactcattt atataaatat ccattaatga tgctttatat tctcttatat
    21121 catagcctaa aaattcagtc aatctctcaa tttccttact aaaatctcca tcttctttta
    21181 ccaaaaattc gtatacatct tccctatatg gatttaaagt tattatttgt tttactactt
    21241 tgcattcgtc agccttccca ataaaaccat ctttataatt attgaataag gcattagatt
    21301 tttttataga ttcttcatca atataagaat gtaccgcagt tatacgattt tctataaggg
    21361 catctacaac agcataatga attttaaaac aatcattgta taaataattg ctcattttct
    21421 ttattgtcga ttctatttct aagaagctag cgtgaccatt ttttttattt ttatttggta
    21481 taataaaagt tttattcaaa ttattaaaaa gtctcttaaa atctaaatat ttttcacaat
    21541 aaacactttt aaaggtatcg atatctatag ttataattcc aaaacttact atagtctcaa
    21601 ttcctttctt aaaagactct tctatatatt tttctcctaa acttaaacct ctatcagata
    21661 tttgctttag attttgaaaa ctatttaaac aatctctctc aaagttttta gatatttcca
    21721 aagcatataa tttaaagatt ttccttatct tattataatt attttttgta tcatcaaatc
    21781 tgataatagc tccatttaca tcaaaagtaa tcattgagtt ttaacctccc cattagtttc
    21841 atcaataaaa taaatttaat ataaacttta taatcataat attaaactta tttatactat
    21901 aattattaat ttgatgataa aagctatttt ccttctaaat aaaaaatttg tctaaagttt
    21961 ctaaaaaatt tagacaaatc ttaggttatt actctttaaa ttatttttaa attattccaa
    22021 atacgccaaa taatattggt ataaatatag ctggtaacaa attggctact tttatattcg
    22081 ttaatttaag tacatttaga ccaagtccta taattaataa acttccagct gctgtcattt
    22141 ctgtgactac aggatcactt aaaaatccat ttaaaaatga tgctcccaca catataatac
    22201 cttcatacaa aaatacagat attgcagaaa acattacacc tatacctaaa gaagcagtaa
    22261 atataattga agctattcca tctataacag attttacaaa taaagtatca tgacttcctg
    22321 taagaccact ttctaatgaa ccaactactg ccatggctcc tatacagaat aataaactag
    22381 atgatataaa accctctgct atagaatctt ttttatttcc tttagaaaat ttactttgta
    22441 aaaaagcacc caacttattt acccatttgt caatatctat taattctcct atcagagcac
    22501 ctacagctac ggatattatt gtcactaatg tgttttttcc ttctagtgct cctgatatac
    22561 caatgtacaa tacacataaa gcaattccat tcattatagt atcgcttact ctttgaggta
    22621 atcctccttt tattataaga cctactatac atccacctgc aattgctaga ctatttgcta
    22681 ttactccaaa cattttttct cccctcgatt agctaattga aatttatcct attattaaaa
    22741 cctaaacatc tatactttgt attggatatt ttaaatattc caaaaattct tcaaaacctt
    22801 ttctagatat atttacagtt ttagtattta tgttaggatg aaaatttaag taatcttctt
    22861 taattacatc tttatctatg taaagctcaa cttttctttc tgtatcattt attaatgaaa
    22921 aaggatttac acttccagga agaagtttaa gtacattgta tagtctttca ggtgaagcaa
    22981 acgataatct agaacttcct atcttatctt taagtgagtt taaatcaact tgtttttctc
    23041 ctttaactac aataagataa tacttatttc ctttggcatt tcttaaaaat aaatttttac
    23101 attgacaccc ctttgttatt ccatctaaaa tgactaattg ctctgctgta tatacagctt
    23161 catgctctac tacatcatat tcaacattta atttatccaa aatatcatat attttagttt
    23221 cattcatgct agattcattt tcatcttgtt cttttttatt atttaaatca ctcatacttt
    23281 tctccttaaa cacttcatca taatatcatt gtgaggtatt attctactat agctattgct
    23341 cttactggag ctccatcagc atcattatac ttaaatggtg ctgctataaa tagaaatttt
    23401 tctggaacat tttctaaatt tgtaagattt tctatgatta tttttccttt ttcaaataaa
    23461 atatgatgca cttcaaatac tgatgtgtca tatctatcca ctgatagcat atctattcct
    23521 atgcctttta tattagtatt agctatatag tttgcagctt cttttgttaa tgtaggataa
    23581 cctacatagt actgcttttt atcccaaaat tttgaccaac ctgatttaaa tattataaaa
    23641 tcactatttt ttatttcatc ttcatatttt tttagatatt ctaattctat attctcattt
    23701 cccttttcaa gttccaatac ataagctttt ccaacaaaat tttctatatc caacttatct
    23761 agggtctcac ctttagtata catatgtctt ggtgaatcca tatgagttcc attgtgagaa
    23821 aatacactaa ttaatgtttc ttgataacca ttttcttcaa taatagctac tttcttgata
    23881 tctggtctat taggctctgc atatacaggc atgtcattat gtgtcacatg agttaaatca
    23941 aaaaccttca tctttatata ccccctatgt ttttattttc tattatatac ctaaattcat
    24001 tttatttcta tcagatttta ttccattata taccatacta ctatatattt tttataaacc
    24061 tcaagggatt taccgaaaaa tcttggaaag aattatcagt aaatataaca tcttcaccca
    24121 aaaaacgttt ataccttcct tcttctttct ttaaagtaat tctctgtttt tgtgaatgat
    24181 tattaaaaac cattatctta agaggaggtt tttttctatt agtctgcact ctttttaaca
    24241 tgtattttat gtgcatatca gcttcaggaa atgaatatcc acaaaatatt aaaaggtcag
    24301 tatctcttaa agttttttct gtttcattcc aaacattact taaaaatatg tttgacatat
    24361 tcttaaaata tgttggtggc actataatag gttctgttaa ctcaccacac tctaaacatt
    24421 ttgtctctga actattttca ataagcttca tgacgccacc ttcatgtggt gtaagagtta
    24481 cactattaca aatggggcaa taaagccaat tcaatgaccc atgtatctta tataatttga
    24541 ttaatggttc ttttggtttt ctccaattta actcctcttt aaaatttgta aactccacac
    24601 cataatcaag cataattggc aaatcattct tatctcttaa tccagtaact gtattgtcta
    24661 tatgtatatc ataatttgca ctaataaaag tagtatcaag aagcaaatca ttttccaaaa
    24721 gattttcaac taaaagttta tgatatttat tatctgttct aggtgtatta tgaattgctt
    24781 cagccattag cagtattaaa tattgtctta aaaaacgtat actatcactt ctgttattta
    24841 gattttctag tccaaaattt ctaaatgctt cttttctttg ttctgctaag tctaaaagac
    24901 ctaaaacttc ttcaaaggta ggaaaatttg cattatctat attatctttt actatatcaa
    24961 tattgaacat ctgtttaaag aaaatataca attctctatt cattttggta ttataatttg
    25021 tgggatttag ttttttaaaa tattcgctaa aaagttcatt ttgaataggt agattctctg
    25081 ctgctgaagc tcctgctcct aaaaatattg ctattttcat atagttcctc ctaatataaa
    25141 tattactcct atttttatat ccattaaata tccatatcat ttattttatg atacaacaat
    25201 aaagctgtct tttactagaa aaaatcctat tacaagacag ccttatatta tattaaatct
    25261 ttaataattc gtaaatagaa agagcatata tttgagtagc ttttataata tcctctacta
    25321 ttaagtattc attagcttgg tgttctgttt ctggttttcc tggaaaaata gctccaaatg
    25381 caacacaatt atctaaagct cttgcgtatg ttgctcctcc agatgaaagt ggtgttccat
    25441 caagaccagt ttcttcttca tatacttttc taagtgtctt aacaagaaga gtatcttctg
    25501 gaacatagat tgaatccaag aaatcatatt cttcataatt taaattgtat ttatctgtca
    25561 ttttctttaa ctcttttaca aaatcatcct ttttgtatgt tactggaatc cttacatcta
    25621 ttcctgcaaa ttctttttcg ttatcaatag ttactctacc tatatttact gttagtttac
    25681 ctgatacatc atccttgcaa ttaggaatta tattgtttcc attagcatct tcaccaataa
    25741 cttctgctaa aaacttaatt atattagaat ctatacctat attattaaga gctatacaaa
    25801 gtctagccac tgcatttata ccagtatcac ttgctgctga atggacactt tttccaatta
    25861 tacagatttt attaccttca acagtatact caaaatttaa tttatcaagt tcttttttta
    25921 atttatcaga gtattttcca atatagattg ctttacctgg aactgcattt aaagcctttc
    25981 caactgacaa ctctatatca cttttgctat cacaagtcaa atgaacttgt aaaagacctt
    26041 tctctgcatt tgttattggg aatctagaat ctggagtaaa tccatagttt ggtatttctt
    26101 cattattttc tttatattta tttatacatc tccaaagatt ttcttcatca gccccaaaaa
    26161 tgaatcttat cctcttatta aagtctacat ttaaatccat aagagctttc actgcataaa
    26221 ttgctgatat tgtaggtcct ttatcatctt gtgttcctct tccatacaat ttaccatctt
    26281 ctaaaacagc ttcaaatgga gggtaattcc agctttctaa atcaccttct ggaaccacat
    26341 caacgtgacc aagtatacct atcatttttt caccttgtcc tatttctgca tatccataat
    26401 atccatcttt gtatactgtc ttaaatccta aagatttaca taagtctaat atacctctta
    26461 aagctttatc tacattttca ccaaatgggc aattttcagt tgcttcagat ataacactag
    26521 gtatttttac actttcttgt attgaagaaa tcatttcatc ttgtagagaa tttacttttt
    26581 ctttaatttg ctctttcata ttatcaccct tcaattttca tcataatata ctaataaata
    26641 ttttgacctt ttaaaaaaaa aatatgctta ttttttcagt ttttttataa attcaatcgc
    26701 ttttaaagtc cccatttttg aatggtcaaa tgtaagtcca ccttgaaaat aaacattata
    26761 aggaggtctt attggagcat ctgcacttaa ttctatagaa gaaccttgta taaatgctcc
    26821 tgctgccatt atgacttcac tttcgtatcc aggcatgtcc caaggaacag gttttacata
    26881 agaatcgact ggtgcagctt cttgtatacc ttcacaaaaa cttattactt catcagcatt
    26941 attaagtcta atacattgaa taatatcact tcttaagtca tcatattttg gtaatacatc
    27001 atatcctaat ttttcaaaag ctcttgagca aaagatagct cccattacag cctgagatac
    27061 tatataaggc gccaagaaaa aaccctgaag tacatttcta gttgttccaa aagtaagacc
    27121 acattctttt cctattccag gagaagtcat tctataagat ataagctcaa tcaaatcttt
    27181 tctaccagct atatatccac ctgtcaatgc aagcccacca ccaggatttt ttattaaaga
    27241 gccagccata acatctgctc caacatcagt aggctctttg gtatctaaaa actcaccata
    27301 acaattatca accattacta tagcttctgg tttaacagac tttattactt ctatagcttc
    27361 ttttatatca ctaattgaaa gtgattttct ccaagaatat ccttttgacc tttgtatcat
    27421 aacgagttta gttctatcat ttattttgtt tttaattcct tctaaatcta agtttccatc
    27481 ctctaaaaaa tctacatcat catatgtaac accatactct ttcaatgaac ctttttcttc
    27541 tcttatgcct ataactccct ctaaagtatc atatggtcta ccagtaacag ataaaatttc
    27601 atctcctggt ctaactatac cttgtataca taaagtaagt gcatgtgtac catttacaat
    27661 tattggtctt acaagagcat cttctgtatt aaatacttta gaataaattt cctctatttt
    27721 ttcacgacca atatcattat atccatatcc tgtagtccag ttaaagtgca tatcacttag
    27781 tttagattct tgcatagctt ttaaaacttt atattgatta tattctctta tttcttttat
    27841 ttcttcaaac ttatctttta tttcttccat tatttcttgc gatagtttaa aagtatcatc
    27901 atctattcca tagtagtctt ttaataattc ttttgtctca ttaagcattt tatcacctgt
    27961 ctctttatta ttttatttaa acatgtaaag gagactattt tactaaaaca aataaattat
    28021 agtttataag ttagtataag tctcctctca atcatcttaa attaaattaa ttatctatct
    28081 gttgttatta ttattgttgt tttggttttg atttgttaga ttaatatact ttccaggttg
    28141 tatagttgat acagcatgtt tataaatcat gttttgttgt ctattatccc cttctattaa
    28201 tattatataa ctatcaaacc cctttacaag ccctttaact tgcactccat taactaaata
    28261 tatagtaaca ggtatccttt ctttccttgc attatttaaa aacaaatctt gtaaatttaa
    28321 aactgtattt ttcattagat ttgtaccctc ccaagattaa attgaacttc taaaatattc
    28381 ctaccttata ttctatataa gttatgacaa attttcaata ttaagttgta tacttaaaaa
    28441 tttatatatt attttataga atctttaata tataagatta tttcattttt taattcgtct
    28501 atattttcat attggtctaa atcaaaccat tttgctgttt tatatctttt aaaccatgtt
    28561 atctgacgtt ttgcatacct tctagaatct cttttaatta tttcaatagc ttcttcgtat
    28621 gtatattctc catccagata ctttaatatc tctttataac ctataccttg cattgatatc
    28681 atatcttttt taaatcccat acttaaaagt ttttttgcct cttcaaccaa tccattttta
    28741 atcataatat caactctcat atttattctt tgatacaaat gttctctatc tctatttaga
    28801 actattatta ttggttgata ttcttcatta aattttaaat cacttgaaaa atcattcatt
    28861 ttcttaccac ttagacaaac ttcaagagct cttatgaccc ttttagtatt gtttttatgg
    28921 attctattag cagattcttc atcaagttct tttaacttat tatgcatgta atctattccc
    28981 ttttcagcaa gctgtttttg aagttcttct cttaattcat tattggcatc agattgtgca
    29041 aaatccatat tatatattaa agagtttaaa taaagtcctg tgcctccagt tactaaaaca
    29101 catttttcct tttcatttat ttcatgtata tatttttttg ctctttgttg aaactctgaa
    29161 actgaaaatg gatagtcagg cttaacttca tctataaggt aatgcttcac tccttgcatc
    29221 tcttcctctg taacttttgc acttccaata tccatatatt catatatttg catagaatca
    29281 gctgaaatta tctctgcatt catatctttt gctaatttta ttgacaaatc tgtttttcca
    29341 acagcagttg gacctgtaag tattataagt ggtatttttt tcataacatt cctttccata
    29401 tattttaaat atccaaatat tttacataat tcttttaaac attttttcta tttctgtctt
    29461 agatatttcg accattatag gtcttccatg aggacatgta aatggattct cacacttttc
    29521 tagctgttcc aataaacttt ttatttcaat atcgtatatt ttatcatttg cttttatagc
    29581 agacctacat gccatagatg caaatctctc tccttttaaa tcataattac ttgtaatttc
    29641 ttctatatta tcaattattt gaagtataaa tttctcagtt tcaggtactc caaatatagt
    29701 aggaacacat cttaccatta tatgattatt gccaaaaatt tctagctcaa aaccaaattt
    29761 cataaataac tccaagttat tttcaatttg aagcatatct atatttgata cttcaataac
    29821 cactgggtct aaaagtattt gcatatttat atcttgtcta tagaacttct ccatatatct
    29881 ttcatatagt attctttcat gtgcagcatg ttggtccaac aaatacatag aatcgtcttt
    29941 gctcaaaatt atataagtat taaatacaac tccaattaca aaatatccgt ataaagaaaa
    30001 cttgcgtttt gaactacaca agtattcttc ttcactgtcc ttaatactat ctcctaagta
    30061 ataatttcct tcatttttaa ttccatcaac ttgaaactct tcttgtatat tatcatttgc
    30121 actatcttca cttagataat taatgttttt tacatcatct tctacacttg catttaggac
    30181 ttcacttact gattgaaatt catttattgg tttttcactt gataaagtaa caacttcaat
    30241 gacttcatcc ttacctttag taatattact atttttagga gtagattcta ataaatcatt
    30301 atttcttaat ttataatctg tacttttttc tctactgtta atagcgattc taggttgtgt
    30361 ttttttatca ctataagtag catattttcc aataagattt gagtgtatta atttaacctt
    30421 taaaaaatct ctaagttcta tatacacttc ttgttctttt tcaaatttaa tttcaagttt
    30481 attggggtgt atatttacat ctatacatga tgggtctact tctatattta agaagcacac
    30541 tgcatgttta cctattggaa ttatagactt ataactttct gttattgcat ctatgattat
    30601 tttacttttt acaaatcttt tatttatata gatatgttgt aagtttttat ttgaacggta
    30661 tatattgtta ttacctatat atccattcat tttaaagtga ttgcacttaa actctacatc
    30721 tattatattt tccgttattt cttttccata tatacttcta atagtgttta caagttttcc
    30781 atcccctgga gtatttagca tctgtttatt gttatttgta tatttaaatt gaatgtttgg
    30841 attacctatt gcaagtttat ttatcaaatc acttatgttt atagtttctg catgagtaga
    30901 ttttaaaaat ttctgtcttg ctggtgtatt aaagaaaata tcttttataa tgatggtagt
    30961 tccattagta gaacctattg gttctttcga tattattttt ccaccttcaa catatatttt
    31021 agttcctatt atttcatctt tagttttagt cgtcatttct aattttgata cagctgaaat
    31081 agatgccaat gcttctcctc taaaaccaag agagtataaa tcatacaaat catcaatttt
    31141 tttaatttta ctagtagcat gtctcaaaaa tgatttttca acttcacttg aaggtattcc
    31201 tattccatta tctgtaatct taattagact ttttcctcca tcaattatat ctatggaaat
    31261 tttatttgca cctgcatcaa tggagttttc aataagttct tttactacag aagaaggtct
    31321 ttctacaact tcacctgctg ctatcttatt tatagtcaaa tcatctagga tgttaattat
    31381 atttttcatt tttacacctc taagatttta tttcttttgc ttttctttga agattataaa
    31441 gagagtttat agcatctaat ggagtcatat ttaaaatatc cagatttaaa atttcttctg
    31501 atagtatatc tctatttaca ctatcaaaag agatttgagt taaggctact tcttttacag
    31561 aatcttcttg attaattttc tctttatcat cattttgctt cattaaagca acttcatcat
    31621 ttaatgcttt aaatttagaa tttaatttat cataatcctt ttttatatgt ttgtagtctt
    31681 ttactataag ttcatgttct atttttagag attcatattt attcttaaaa ttactttgat
    31741 ttaccttaac taattcttcg tccaaattac tattaattat attattgttt tctttatctc
    31801 catttattgc aacactgttg taaacatgat ttttttccaa gtctttaagt atatattttg
    31861 ctctttctat gacttcgtct ggtaatttag ctaatttagc aacatatatt ccataactct
    31921 tgtctgctcc ctgaggaatt atttttctta aaaatattat tccttcccca tcttctttga
    31981 ctgctattga ataattttta acctctttaa actcttcttc taaatcagta agttcatgat
    32041 aatgtgttgc aaataatgtc ttacatctaa tatttttttg aatatattcc actattgacc
    32101 atgctaaact tataccatca taggtactag ttccacgacc tatttcatct aatataacta
    32161 aacttctctc tgttgcattt tttaatataa gggatacttc gttcatctct accataaaag
    32221 tagattgacc ttgagataaa tcgtcactag ctccaactct tgtaaatatt ctatctaata
    32281 ttggaatatc agcagactct gctggtacaa atgagcctat atgtgccata agagctatta
    32341 tagcagtttg cctcatatat gttgatttac ctgacatatt aggaccagtt attatgttaa
    32401 taatgttttc tccacgattt aaataagtat catttggaac gaaattttcc tctcctacta
    32461 tattttcgac aacaggatgt ctaccatttc ttatatcaag tttattattt tcatttattg
    32521 ctggttttac gtagttattt atatgtgcaa ctgttgcaag ggacacaaaa acatctatat
    32581 tagctatagt cttagccact ttttgtattc tatctatgtt tttatatatt gtatctctta
    32641 tctctacaaa tatctcgtat tctaaagatt taatcttttc ctctgcatgt agtatctttt
    32701 cttctatttc ctttaattct ggagttatat atctttctgc attactaagt gtttgctttc
    32761 ttatatatgt ctcatctagc tttgcctgtt taaagttggc ttttgttatt tctatataat
    32821 acccaaaaac tttattaaat cctattttta atgatttaac tcctgttttt tctctttccc
    32881 tgttttctat ttcctttaca agaaaggctc cattcttaga aatctctctt agttccttaa
    32941 gttcatcact aaaatctgat ttaattatgt ttccatcttt tatggttatc gtaggttctt
    33001 ctagaatagc ttcatctatt aaattgtata tatcatctaa tttatccatt tctgaaacat
    33061 actcttttag gatttttgca tttgataaat ttatagtatc ttttaaatta ggtaactttt
    33121 ctatcgaatt ttttaagtga attaactctt ttggtgttac tctctcaaat gctatctttc
    33181 cacatattct ttctatatcg tatatatttt taagtatatc atttaggtct tctcttaata
    33241 taaagtcatc ttttatttct tctattacat ctaatctatt ttcaatttta gatttattta
    33301 tcaaaggttc ttctacatat tttcttaaaa gtctacctcc cattgctgta gaagttttgt
    33361 caagtacatg taataatgaa ccttttttct tattccctct tatagtttga gtaagttcta
    33421 aattagttct agtaaacata tctagaacca tatattctga tgaattgtat atatttatat
    33481 tattaatgtt agatgttatt tgtttttgag tattgtatat ataattcaga agaattgata
    33541 agcttgactt tattagacct ttatcatcaa actttagttt ttgtagataa acatctggaa
    33601 aatattcttt taatatattt atatccaaat agttatcact aaaactttca tttatgtata
    33661 tattacttac agtagctata tctctaagct tttctataaa gtctaaatca tttataatta
    33721 tttcagttgg atggattttt gcaatttctt caattacctt atcttcattt aaacatgttg
    33781 cattagtttc tcctgtactt atatcaacat aagttagacc tatattagta ccatccttat
    33841 acaaagataa taaataatta ttctttttat tttcaagtaa atttccatct aaaactgtac
    33901 caggagtgat gacccttata acctctcttc taacaattcc ttttgctgta gatgggtctt
    33961 ccatttgttc acctattgct actttatatc cattttcaac taattttgat atatatgaat
    34021 tggctgagtg gaaaggaaca ccacacatag gagcacgttc ttctaaacca caagcttttc
    34081 ctgtcaatgc tatttcaaga gcttttgaag ctactaatgc atcttcaaaa aacatttcat
    34141 aaaaatctcc aagtctaaaa aacagtatac agtctttata tctatttttt acttcaagat
    34201 actgtttcat cataggagtt aatttattca tatctatttg cattatcaaa cctccttctt
    34261 ttctaattaa atattatatc atagtagatg attattatta acacaatata taaaattttg
    34321 acatataact aaaaacaatc caaaatgtat actcacattt tggattgcta agtttaatgc
    34381 ttattaaata aaaatatttt tttttacaat aaagatatta tttctttttt aagtttaatg
    34441 atattttcaa ggttatcttc atttaacttt ctactatcta taacaaattc tttctttact
    34501 attgatggtt tttttgaaat aacataaatt ctgtttgata gcattatagc ttcgtcaata
    34561 tcatgagtta taagaagtat tgttgaattt acttttttct taacatctaa aagccatttt
    34621 tgcatggatg ttttagttaa tgaatcaagt gccccaaaag gctcatctaa aagcattatg
    34681 tcattagaat ttataaaagt tctcaagaag ttagctctct gtctcattcc tccagaaagt
    34741 tctgaaggat atttatcttc atatccgctt aatccaaaca cctcaaaata aggttttacc
    34801 attgacctag ctttactctt tttttctttt tttaaaatta gtggtagtga cacattatct
    34861 attatagttt tatatggcaa gagtaaatct ttttgataca tataactata cttaccattt
    34921 atattcactt tgccactatc ataatctgta agtttagtta taatattaaa aatagtactt
    34981 tttccacatc cacttggccc taatatagaa acaaactcac cttcataaac atcaatactt
    35041 atatctttta aaacttcaat atctttataa gtttttgata cattttctat agatactttt
    35101 acttttttat tgtggtaaaa attcatttgt gtaagcatca cttgccttca tatccttatc
    35161 tattaattta tattctttta agaatgatgt atagttgttc catacactat ctttcatttc
    35221 tccccatcgt ggtgcatcat ctttgtactt gcttgctaaa tattcttgac tctttaaagc
    35281 tagtttttca tctacttcag gtgcatgttt tactaaaatt tttgctgatt cttctggatt
    35341 ttttattgca tattcataac cttttgttgt agcttttaag aattttttag caagctctgg
    35401 attatcttta agcacagttt cattagaaat tataagtggt gtataataat ccaatctctc
    35461 atctaaatct tttactggta taaagtttaa gtcatatcca attaaatcag ctttaactgc
    35521 atcccaacct tcaaatatcc atgcaaaatc tacagttttc atagctgcaa agaaatcatc
    35581 ttgaccagta ttaactatct ttaacttatt aaaatcagct ccatttttct tcataactgc
    35641 tttaaaaaca gcttcttctg atggagagcc ccaaccacca taagtttttc cttcaaaatc
    35701 tttagctgtt gttatattct tttcttttgg tgatgcaaac ccagaagtat tatgttgtat
    35761 aacagttgct acagctttta ttggaagtgg atcttcacta gtctttgcat aagtaacttg
    35821 ttcttgatag cttataccaa agtcagcttt tcctgttgcc actaaagttt ctgctccact
    35881 ttcaggtggt tgtactattt caacatccaa accttcttct ttatagtaac ccttatcaag
    35941 tgctacaaat aatccagtat gatttgtatt tggtgtccaa tcaagcacca tagttacttt
    36001 tttagaagaa gtatctttat cattgttttt gtctttattg ttatttgaag aacatccagt
    36061 tattgttgcc aatgtaaaaa cacttactaa acctaatatt cctaactttt taaaattcat
    36121 aattgcctcc ctcaaatacc tttaatctat taatctatgt cttataacaa ctttttttac
    36181 agcctgtaca attcctacaa acattaaact aaatataact actaatatag tagatgcaaa
    36241 tactttatca agtgcatatg cactttttga cctaagcata taaacaccaa gtcctttcgt
    36301 tccacctagc cattctgcaa cagttgctgc cattacagca tacgttgcag atattttaag
    36361 tccagaaaaa aatttatcca ttgccatagg aaatttaagg tgaatgaatg tattaacttt
    36421 atttgagtcc atagtcttaa ataaatttaa ataatcttta tcaatattct caatgccatc
    36481 tacaaaactt attaatatag gaaaaaaaca agtaagcatt accatcaata cttttggcat
    36541 tgttccaaat ccaaaccata tcataaagag tggtgctata attattgtag gtatcatctg
    36601 tgtaaccaac ataataggat aaagacattt ttttattatt gatacaaaat ccatcaatat
    36661 tccaattacc aatgatatta taattgcgac tataaaacca agtattgctt ctcctatagt
    36721 aaccacagca tgcatggata aattttgata atctttaaaa aacacattaa ttatatctac
    36781 tggtgtgggt agtatatact gaggaacttc taaaactcct accatagatt gccaaagaat
    36841 aagtataaac aaaaatgttg ctattggata tatactatct ttgactttat ctatgatttc
    36901 taagtttttc atctatagtc agacctccag ctttttcatc tactctagct actgttatga
    36961 ctctttcaca tccaagatta aaagggattt tatgtacctt ttttaataat tcaaaaactt
    37021 catcaaaatc accttcaaca gtagtgctca ttgctcctat ttcacattta agaccacttt
    37081 tttcaatttc agcaatacaa gcatctacta ctttatacat ctcttcttca cctttgtaag
    37141 gtcttagtgg cattactgct atgtctgcga ttaccattta aatcactcct ttaaaaataa
    37201 ttaataccta ctaggtatat tttgtttttt attaaaaaat tttattacta aaaagatatt
    37261 atatttaaat aaaagaaaag tcttatccaa aataggataa gacttatttt tatcttaact
    37321 ggtctcccta cactggcatt atccaaacag gttcaaaggg tcgagataaa atctcctctc
    37381 agcacattag ctccccgaaa tatattatat cttttctact tgattatact acacttatca
    37441 tattttaaca a
//
LOCUS       DBGJFF010000027        37341 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.027, whole genome shotgun sequence.
ACCESSION   DBGJFF010000027 DBGJFF010000000
VERSION     DBGJFF010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 37341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37341
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.027"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            63..1193
                     /locus_tag="ACOIAF_001396"
     CDS             63..1193
                     /locus_tag="ACOIAF_001396"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0051144 - propanediol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-propanol dehydrogenase PduQ"
                     /protein_id="HGZ2668412.1"
                     /translation="MNNFEIKTRIKFGEGSLKALQELKNKNVLIITDPFMVKSKTIDK
                     ITSNLINSTYQVFEEIVPDPPIEVVVAGIEAFKSINPDVIIALGGGSAIDAAKSIMDF
                     SKKILKINEVEFIAIPTTSGTGSEVTSFAVITDSQKKCKYPLVSDDLLPDMAILDPEL
                     VKTVPNFITADTGMDVLTHAIEAYVSTNSNDFSDALAEKAIKLVFKYLIKAYKDGNDI
                     EAREKMHNASCLAGMAFNQASLGINHSIAHVLGGKFHIPHGRTNSILLPHVIEYNSSI
                     VGYTNTTYSDTAKKYAQIAELVGLGSANIRISVRNLVNEIKKLQKEMNMPTKLTQCKL
                     SLEDITNEEREIAKLAIKDTCTLTNPKVPTEDDIINILKNIK"
     gene            1434..1784
                     /gene="eutS"
                     /locus_tag="ACOIAF_001397"
     CDS             1434..1784
                     /gene="eutS"
                     /locus_tag="ACOIAF_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HGZ2668413.1"
                     /translation="MTEESKQRVIQEYVPGKQVTLAHIIANPNEDIYKKLGLVLDKKD
                     AIGILTITPSEASIIAADVATKASNVSLGFIDRFSGSVVISGDVSSVESALNDVLEVL
                     GNMLNFSSTKITRT"
     gene            1792..2241
                     /locus_tag="ACOIAF_001398"
     CDS             1792..2241
                     /locus_tag="ACOIAF_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HGZ2668414.1"
                     /translation="MKNIIFMGKTGSGKTTLCQKLDQLELKYKKTQSVELYNNAIDTP
                     GEYMENRNLYNALITTSADAKIIAIVYDATQEENYIAPGFASMFCKDVIGIITKINKA
                     TENEVNIAYERLSLAGASQIFKIDTVDNIGLEKLFKYLEKYKNIDEV"
     gene            2343..2918
                     /locus_tag="ACOIAF_001399"
     CDS             2343..2918
                     /locus_tag="ACOIAF_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HGZ2668415.1"
                     /translation="MGKKILLVDDEPLLRMDTKDILESHGYEIIGEASDGFEAVEMCK
                     KHKPDLVIMDIEMPILDGIKAGKIICKENLAGGVLLLTSFEDKEYVEKAKSIGAYGYL
                     VKTASEKSLIPTIEMCLNKVNEFEKMKKDLEKVNSKLNERKLVERAKGILIREFKISE
                     DEAYTRIRKLSMDKRTTMMEIAKMIIIGYDD"
     gene            2911..4320
                     /locus_tag="ACOIAF_001400"
     CDS             2911..4320
                     /locus_tag="ACOIAF_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal domain-containing
                     protein"
                     /protein_id="HGZ2668416.1"
                     /translation="MTNTIKKISTAQTYLNNNEIEKIIEVSKSLDLMASFYEADVFID
                     VLSKFDDEAIVVAHGRPKEKSMYCENVIGKKALIENEPGVIKCLKTGERVRDIKALTQ
                     EYKLVKQNIQPITLDDKVLAVLIVEKDISSEVKNEFNASEDEENKNLHQFIKLIGNND
                     FLVDNLNSAILIFDKNGKLKIKNRIATEIYKNLGFGYDIQDMHYNDLDIETNTFENIL
                     MDENYDETNEVSIGNMFFKIKTIYIKDDEFKVCKIVQDISDLKRKEEELVLKTVAIRE
                     AHHRVKNNLHTVISIIKKQSRLSQNDEVKQCLDNIINRVFAILSTHYLLSKEVDNNIS
                     IREGINLLVSNIQGGYLFSKDIDIRITGEDFKISGEKATAILLVINEILQNCYDHAFS
                     DREHGTIQILINKDNDYRNIAIIDDGIGFDSNNINKNSLGTYIIDSYITQMLKGNIER
                     KSSENGTEVLITIPTQINI"
     gene            4504..5937
                     /gene="eutA"
                     /locus_tag="ACOIAF_001401"
     CDS             4504..5937
                     /gene="eutA"
                     /locus_tag="ACOIAF_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HGZ2668417.1"
                     /translation="MREELLSVGIDIGTSTTQLIFSKLIVENTESNFSVPRISIVDKE
                     IIYKSKVYFTPLISNNEINGQQIKDIVENEYKKANISKEDIHTGAVIITGETARKENA
                     NDVLHTLSGFAGDFVVATAGPDLESIISGKGAGAHIYSKEHSTTIVNIDIGGGTSNIA
                     SFKRGEVTDTGCLDIGGRLVKIDKISKKITYISPKIDKIIKDNNLNLQVGTMATVENT
                     KPIVDIMVDLLMESIGLRKQTPLFDFIITNKSISIDENIKNVSFSGGVADYIYYDGEI
                     KDYFEYGDLGILLGQAIKNCEYLKKVNVVKSMETIRATVVGAGSHTTEVSGSTITYTS
                     EKFPLKNLPVLKLSIDEETGTSKQISEAIKNKLNWFKLENDLQKVAIAMIGKKNPTFL
                     DIQEYAKGFVDGMQQIIENDLPFIVIVENDMAKVLGQAIYALLNYEKEIVCIDSVKVE
                     NGDYIDIGKPIVEGKVLPVVIKTLVFN"
     gene            5955..7319
                     /locus_tag="ACOIAF_001402"
     CDS             5955..7319
                     /locus_tag="ACOIAF_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HGZ2668418.1"
                     /translation="MILNTKLFGRTYQFKGLYDVMAKANEEKSGDKLAGLAAESAEER
                     IAAKVVLSHIKLEDIRNNPAVAYEEDEVTRIIQDAVNEKVYKEIKNWTVSELREWLLD
                     SNTTSLDIRRISRGLTSEMIAAVAKLMSNMDLVYAAKKIKVTAHCNTTIGEQGTLSAR
                     LQPNHPTDDPDGILASLLEGLTFGIGDAVLGLNPVDDSVESVTKVLKRFEEVKQKYQI
                     PTQTCVLAHVTTQMEAIRNGAPADLIFQSIAGSEKGNEAFGFNGDTIEEARQLALKHG
                     TATGPNVMYFETGQGSELSSEAHHGIDQVTMEARCYGFAKKYEPFLVNTVVGFIGPEY
                     LYDAREVVRAGLEDHFMGKLHGLPMGVDVCYTNHMKADQNDMENLAILLTTAGCTYFM
                     GIPHGDDVMLNYQTTGYHETASLREMFGLTAIKEFDAWMEKMGFSKDGKLTELAGDAS
                     VLLG"
     gene            7332..8213
                     /gene="eutC"
                     /locus_tag="ACOIAF_001403"
     CDS             7332..8213
                     /gene="eutC"
                     /locus_tag="ACOIAF_001403"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008851 - ethanolamine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HGZ2668419.1"
                     /translation="MNEKDLKALVEQLVGQMVGELDTNVVSETVKKATEVVVDNNACI
                     DDITEVDIRKQLLVKNPKDAEAYLDMKAKTPARLGIGRAGTRYKTETVLRFRADHAAA
                     QDAVFSYVDEEFIKENNMFAVETLCKDKDEYLTRPDLGRKFSPETINNIKSKFGTNQK
                     VLILVGDGLSSAAIEANLKDCVPAIKQGLKMYGIDSSEILFVKHCRVGAMDHLGEELG
                     CEVICMLVGERPGLVTAESMSAYIAYKPYIGMAEAKRTVISNIHKGGTTAVEAGAHIA
                     ELIKTMLDKKASGIDLK"
     gene            8233..8886
                     /gene="eutL"
                     /locus_tag="ACOIAF_001404"
     CDS             8233..8886
                     /gene="eutL"
                     /locus_tag="ACOIAF_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HGZ2668420.1"
                     /translation="MKNDMIRPNILGIKIISNISPEMAQKLELKSHHKSLGLITADCD
                     DVTYTALDEATKASEVDVVYAKSMYAGAANASTKLAGEVLGIIAGPSPAEVRSGLNAV
                     VDFLEYGATFISANDDDSIAYYAHCVSRTGTYLSEVAGIREGEALAYLVAPPLEAMYA
                     LDAAMKAADVKMCELFAPPTETNFGGALLTGSQSACKAACDAFAEAVKSVADNPTGF"
     gene            8897..9598
                     /locus_tag="ACOIAF_001405"
     CDS             8897..9598
                     /locus_tag="ACOIAF_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HGZ2668421.1"
                     /translation="MDAIGLIEVIGYVAAIEASDACLKSANVNIVRVDKVGAGIVTLT
                     ICGDVGAVKAALEAGEIAASRVGTLRTSHIIPRIHNEVTNALFKTKEPKVCEVSEDKE
                     KNFEITLEIADNVSKNLSEKENVILDVNENSGDTENLYSDIDKTNKKIETVDDSLTEA
                     NEEKIENTNDKEVLEQVKIENSDFDRDDLSKNSVKKLKAMAKKLDSSITYKDLNVLKK
                     EELINLVKKLSRRDR"
     gene            9601..11070
                     /locus_tag="ACOIAF_001406"
     CDS             9601..11070
                     /locus_tag="ACOIAF_001406"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HGZ2668422.1"
                     /translation="MTLLDKDLVSIQEVRTLVRRSKVAQEKLSKMSQEQIDKIVKAVV
                     DAAFNNATKLAKMANEETGFGKWEDKVIKNTFASMGVYETLKNTKTIGIIGEDKEANT
                     IDIATPVGVVAGLIPSTNPTSTVIYKTIISLKAGNTIVFSPHPNAKNCILETVNIISD
                     AAIKAGAPEDSILCITNPSIVGTNELMKHKDTNLILATGGEAMVRAAYSSGTPAIGVG
                     PGNGPAFIDKSANVKLAIKRIIDSKTFDNGVICASEQSIVIEKSMEETVLKELKNQGA
                     YVLNEEESERLAKFILRANGTMNPQIVGKSVEVIANLADLTNVPKNTKVLVARENKVG
                     HNVAYSREKLTPILAFYVEENVEAIMNRCREILLNEGAGHTFSMHVNDEELVKRFALN
                     MPVSRILVNTPSALGGIGASTNLVPALTLGCGAIGGSSTSNNIGPLDLINVKRVAYGI
                     KEIEELGRDKSQVEKSMFDSMDKEELINLLVQKLVKELA"
     gene            11166..11453
                     /gene="eutM"
                     /locus_tag="ACOIAF_001407"
     CDS             11166..11453
                     /gene="eutM"
                     /locus_tag="ACOIAF_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HGZ2668423.1"
                     /translation="MASANALGMIETKGLVGAIEAADAMVKAANVQLVGKEQVGGGLV
                     TVMVRGDVGAVKAATDAGAAAAERVGELISVHVIPRPHFEVDAILPKVSAE"
     gene            11580..12341
                     /locus_tag="ACOIAF_001408"
     CDS             11580..12341
                     /locus_tag="ACOIAF_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin adenosyltransferase"
                     /protein_id="HGZ2668424.1"
                     /translation="MGVLTENEVKRKIGKNKNNITEFYIEKGQIITPSAKSYLAERSI
                     ELKYVDKLSDVNNEPKEVVKEVVKEKKYKYTTVFGAKLDEKPEHMTQLNGNLLVFKDH
                     KRIILRGKIDSLESEILKVQILCQKLDMPKLVKELQEVLDFVRNIMRCEVLDEKLGDF
                     KLLGLTPQELREQSHYPKKYFGIGHEFPSYEMGEVVVAINSIRSNTREVELAAYEAFK
                     GEYGNVHREDLIKALNRLSSVFWIMIYKVRTNKYK"
     gene            12354..12983
                     /gene="eutD"
                     /locus_tag="ACOIAF_001409"
     CDS             12354..12983
                     /gene="eutD"
                     /locus_tag="ACOIAF_001409"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization phosphate
                     acetyltransferase EutD"
                     /protein_id="HGZ2668425.1"
                     /translation="MENLLNEIVEEVITRVKKEAFIEVEASGRHVHLSREDVDKLFGE
                     GYTLTKLKDLSQPGQYACKERVTITGPKGSIKNVVVLGPCRNETQVEISLTDGSTLGL
                     KAPIKQSGDLEGTLSIKISTQYGEVELDKGLMVAKRHIHMTPKDAEKFNVCDKEIVEA
                     KVMGQRPLTFDDVVIRVSDSFKTYMHIDYDEANACGYSKGTVAKIIKKA"
     gene            13025..13750
                     /locus_tag="ACOIAF_001410"
     CDS             13025..13750
                     /locus_tag="ACOIAF_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02536 family ethanolamine utilization
                     protein"
                     /protein_id="HGZ2668426.1"
                     /translation="MNYDNLVDLITEEIYKKINSNEIKISNKPKAVIVFEQDNNKFNL
                     LKDEFEIVEFDKSIRECEIVIVSRLCMRGLSNIALGNSTSDEERFILKMIMKGKKVYV
                     LDEGIEYKKYKDTAPKALYKKFMSFEDEICKFGVEIIKDLNVITKNKLNIKKELESTR
                     GSKKEDTKVLDKDVLNLTSVESVKIDNEFSLDLRNKKLISEADLRKPTINGVKNILVN
                     KKSIITPLAVDFMRIHHLKLKKL"
     gene            13763..14035
                     /locus_tag="ACOIAF_001411"
     CDS             13763..14035
                     /locus_tag="ACOIAF_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HGZ2668427.1"
                     /translation="MDIGKVVGNVWATRKDEKLCGQKLLVVKILKTKDTYKEGFMVAA
                     DNAGAGNGDLVLISKGSSARESIEKSHVPVDATIVGVVDSFEVFDE"
     gene            14028..14579
                     /locus_tag="ACOIAF_001412"
     CDS             14028..14579
                     /locus_tag="ACOIAF_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HGZ2668428.1"
                     /translation="MNKSIGAIEFKSIAKGIEVSNEMIKKSSVDVLYLKSICPGKFLI
                     IVGGETSYINECVDYGIKLGEGYIVDNFVINAISQEILDGFKNKYQKLDSIVSIGVVE
                     SSKVCAGIKMLDKTLKSGDLVLVKLQLSFAIGGKLVYIVAGDLSSLEYALKESENVVR
                     EKEVIYKTVIPSVDSQIIKSLIK"
     gene            14591..15682
                     /gene="eutH"
                     /locus_tag="ACOIAF_001413"
     CDS             14591..15682
                     /gene="eutH"
                     /locus_tag="ACOIAF_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HGZ2668429.1"
                     /translation="MSINEIIIYVMVVFMVLGAIDKCIGNKFGLGEQFEEGIMAMGSL
                     AVAMVGVICLAPVLADVLRPIVVPVFNILGADPAMFAGSLLANDMGGAPLALELAKDP
                     NAGLFGGLIVGAMMGPTIVFIIPVALGIIEKEDQKFLATGILAGIITIPIGAFVGGLV
                     AGFEVMMVLRNLVPIIIFAVVIALGLLKFENAMIKGFTYFGKGVVIVITLGLAAAIVE
                     ALTGIVVIPGMKPISEGIEIVGDIAIVLAGAFPLVFVITKVFNKPLMALGKVLGMNDV
                     SAAGLVASLANCIPMFGMMKDMDNRGKIINVAFSVSAAFVFGDHLGFTAGFNSNMITP
                     MIVAKLVGGITAVLLAMVIANKTLKKENV"
     gene            15684..16157
                     /locus_tag="ACOIAF_001414"
     CDS             15684..16157
                     /locus_tag="ACOIAF_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HGZ2668430.1"
                     /translation="MDISNIDKNLIETLVRQIIEEKISGTKDTVDFVRNKDISGITSI
                     KLPTVKVSESDRLDTGNPSDVVYTKDLFTLEESPRLGCGMMEMKETTFDWTLNYDEID
                     YVIDGTLDIIIDGRKVSASSGELIFIPKGSKIQFSVPDYARFIYVTYPADWASQN"
     gene            complement(16277..16372)
                     /locus_tag="ACOIAF_001415"
     CDS             complement(16277..16372)
                     /locus_tag="ACOIAF_001415"
                     /inference="COORDINATES: protein motif:HMM:NF012392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGZ2668431.1"
                     /translation="MGYPNVEHFIRLFNKKYNMSPSKYRKNKYKI"
     gene            16673..17194
                     /locus_tag="ACOIAF_001416"
     CDS             16673..17194
                     /locus_tag="ACOIAF_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HGZ2668432.1"
                     /translation="MEEIVKKEQASMNDIFLYLKAKSNSLYKFVILYNEFINKPKDYG
                     TGELINMVEVHMLTAIEENPGISPTQLAKIWNRTKGAISQTISKLEKKGYITKEKKEG
                     NAKTILLYPTTSGVELSKAHKLYDTIDVTRTFEYLIKECTLEEIDSFYKVIDLFINLL
                     EESPQENNDSEKV"
     gene            17573..18748
                     /locus_tag="ACOIAF_001417"
     CDS             17573..18748
                     /locus_tag="ACOIAF_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HGZ2668433.1"
                     /translation="MNLKQLSEKYEEYVIQMRREFHKYPELSFKEIRTTQRIREELQK
                     IGVPFVSIEPNIVIATIQGKNEGKTIAIRGDIDALPMQEDSDVEYKSEYDGIMHSCGH
                     DAHGAMLLGLAHMLNDIRKDLKGRVLLVFQAAEEVGGGHKEIIQYFKENGGPDRLIAT
                     HVWSDIEAGKISVEPGPRMAGGSGWRIDITGRGGHGSRPDQSIDPIKPACDIVLKVSS
                     IPVNHISVLEQCVVHACAIHGGTTIGNVIPNNAEVIGGYRYFTEESGKKVFDIIKQMS
                     NGVGQVYGVDVKVTHTGGIGPVINDEEAVKMAKEIVSNIDGLELDSYEPICASDCYGE
                     ILKEYSGFYCYLGVGNKEKGIIYAQHHPKYNIDEDTLKLGCEFMAKYAVEFLNKNNN"
     gene            18782..20320
                     /locus_tag="ACOIAF_001418"
     CDS             18782..20320
                     /locus_tag="ACOIAF_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HGZ2668434.1"
                     /translation="MQGALKENKKKSGGGFLGWIEKVGNKMPHPLALFTYITLIVVAL
                     SAIASFLGFSATSPATGKLIKVMNLISAKGLVLFMQEFVKNFQNFPVLGVVLVMAVAT
                     GVCEKTGFFSTAIKMSLSKVKGNAVVFIIAFIGVFANQAGDAAFVLVPTLAGAIFYGL
                     NRNPLAGIFCGFASVGGGFATAVIPGGWDVTLTPITVSAAQTIQPAFDMTLLASYYAL
                     FVSAFIVATVSTIVTVKFIEPKLGKYTGKPEGIDDNQGFEVTKEQAKAAKLAGFTVLA
                     YVALLIALCIPANSFLRSPEGSLIFGAPLMSCLQFFIVILFFLPGIVYGMRVGKIKTV
                     KDLNDILCQSMAAMAPFIVLAIVIGQFLTLFSVSNLAQVLAIKGGALLNSLPLPPQAI
                     ILLFLVLVAFINIFVISGSTKWMIFGPVFVPMLMQLNIHPAFTQFVYRLGDSVTNHLS
                     PLNAFFIILLATVQKYDKNAGMGTIFSAMIPYTIGYFVVLAVLVVVWMTIGIPVGIGG
                     QVWL"
     gene            20352..21554
                     /locus_tag="ACOIAF_001419"
     CDS             20352..21554
                     /locus_tag="ACOIAF_001419"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HGZ2668435.1"
                     /translation="METTKIRYNFDEVIDRSGTNCLKYDLKSQFMPESPEDALPLWVA
                     DMDFACAPEIINAMHERIDRKIFGYSSQQTDDYYEAVCGWFKRRFDWKIEKENIFFSP
                     GVVPALGFFTQILTQKGDGIIIEKPVYYPFEDKITNNERKVVNSPLKFEDGHYSMDYD
                     GFEELAKDENNKVFILCSPHNPVGRVWKKEELRKIVDICKKYDLWIISDEIHCDIIRK
                     GEKHIPLEVACPDYKDKIITCTAPSKSFNLAGMQLSNIVINNKELQEKWMLETDSKLA
                     IFLPNPFAIVATKVAYNDCEDWMNQVNEYIDGNIKYVEDFVNEYMPKVKIIKSQGTYL
                     VWLDFRGYGLDAKQLEYVMLNKAKVLLDEGYIFGEEGAGFERINVASPRTIIEECMNR
                     IKCAFEGL"
     gene            complement(21721..22320)
                     /locus_tag="ACOIAF_001420"
     CDS             complement(21721..22320)
                     /locus_tag="ACOIAF_001420"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2668436.1"
                     /translation="MNDNIVEVKNINMNFYTKEGELEVLKDVNFNLKEGEILTLLGPS
                     GSGKSTILNILTNLLKPTSGDIKITGNIGYMFQKDNLLEWRNIMDNITIGLEIQGKKD
                     KKSLDRVESLLKTYGLWDFRSMYPKELSGGMRQRVALIRTLSVNPDILLLDEPFSALD
                     YQTRLLVCDDVYSIIKNENKSTILVTHDIGEARCIKVQL"
     gene            22490..23848
                     /locus_tag="ACOIAF_001421"
     CDS             22490..23848
                     /locus_tag="ACOIAF_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668437.1"
                     /translation="MSNIITNLIESIFTNPITILFLLAITVYSVFKIIENNRVYSFVS
                     SKLDEINRDYKTSEFYKKVRDDYYTYSKENPYADVNITSFIEEVVSDLKHNNLPLLEK
                     IRSIKNSSSISILLGVLGTFVGLSTMLLCVDTKDIINSLPSTISSMQTAFTTSIFGVV
                     FSLIIGYFTKIKDCEHVLIQIMLKSENLLTSEITHFKSERMDLKVEEVKNTIKQISKS
                     IEAIEKFDKISKDLNDFNDEFISGIEALKSLLEGSQSSIKTFDQSVRKLDKQFNILNI
                     KFVKLFDKYDNQDNINKEILFDIKESSKNIYNATESQFKIRDYIKNINAGFALYERSA
                     QDLLTKLMTHENKISQNQKILLDEKFTLDDSIKNLSSIIENFSNDLQVKLDMMFENSL
                     DIQDKLDVMFNNSFMNDEVPLDSEELFKDDVNNVFNPFSEEIYEIEDKEIKVIGEDEL
                     NE"
     gene            23841..24473
                     /locus_tag="ACOIAF_001422"
     CDS             23841..24473
                     /locus_tag="ACOIAF_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HGZ2668438.1"
                     /translation="MNNKYRRTVNREFEKSSFWPTFTDLLSTVLMVVILILFSSESIS
                     GSVEQDLAKNVNASVEETFKKSKMPVKVDKSNGQVTFGERTMFDVDSDVLKPEAKEML
                     KMFVPKYIETIYKDYGDYISKIVIEGHTDDVGSYIYNLDLSQRRAYSVAKFIVGDEIG
                     DYKYKDKVTKHIIAIGRSKAELIKNSDNSVNRDASRRVELKYEININQNK"
     gene            24802..25959
                     /locus_tag="ACOIAF_001423"
     CDS             24802..25959
                     /locus_tag="ACOIAF_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HGZ2668439.1"
                     /translation="MIEISIKNKKNSKIKTLILLLIVSISIYLTGCATSDNGNNQSNN
                     DDSLSSSISSDKNVKIHFINTGNSDAILIQDGKTFTLIDGGDNDDEKLMVDYLNNQGV
                     KDIKYLIATHSHADHLGGLDSVVKNFNIENVFVSNGSAETKSYRDFINALANKDLSPS
                     VPLENNKFYLEDSYFEVLNTNGGDSTNEQSLVLVYTNGNDKVLFTGDAEEGTEKEILP
                     KLEKVDLLKVAHHGSRSSSSQEFLDKINPEYAVLLVGKGNSYGHPHQETMNKLKKMGV
                     KVHRSDECSDIIFESTGDGVFTSCKDGSYNKGVREDGNSGKSNSNTSKNNSFKNKQNT
                     SNSEEIVYFTPKGKSYHSTKNCSGLSRSKKILSGTIAESKKNDPCDICYGK"
     gene            complement(26125..26760)
                     /gene="lexA"
                     /locus_tag="ACOIAF_001424"
     CDS             complement(26125..26760)
                     /gene="lexA"
                     /locus_tag="ACOIAF_001424"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HGZ2668440.1"
                     /translation="MYLDLTEKQVLILEFIKSQITLKGYPPAVREICTAVGLRSTSTV
                     HSHLNKLEKLGYIRKDPTKPRAIEVLERSKVNDVTGANQEIIELPLVGQITAGEPILA
                     QQNIEEYIPFPASLVKGSNNFVLRVKGESMINAGILDEDYVVVDKKNTALNSQIVVAL
                     INGESATVKRFFKEGNLIRLQPENDFMEPIMLNDSEVEIVGIVTGVFRVIK"
     gene            complement(26864..27991)
                     /locus_tag="ACOIAF_001425"
     CDS             complement(26864..27991)
                     /locus_tag="ACOIAF_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGZ2668441.1"
                     /translation="MNNDKKIIKVHNKSDKPDNIVIKENELIEKCLLLENKLPAFMKD
                     YFIYLKGSVAVSTRLAYLEDINFFCSYLIETKELTNADCVKDIKEDDFNTIKSRDINL
                     FLGDYCSRYYKNTEKNTLIFENNNRALARKKSSISTLFKFLYRNSQIDNNITDGFNPI
                     KLPKPQPDAIKRLEIDEVAKMLESVETGEGLTEKEKVYWRKTKLRDKAILALFVTYGL
                     RLNELRELNISSFNFSRGEFKIYRKRGKEVLMPINHTCEHVIKDYLQNERTRDELLND
                     EEKDALFLSLQNKRITAKAIRTLVKKYTSIPLDTTRDNGYSPHKLRATAATSLIQTGF
                     SIYDVQNLLDHDNVTTTQLYAAHKKNVKRDIVKNFEWIEDD"
     gene            complement(28312..29157)
                     /locus_tag="ACOIAF_001426"
     CDS             complement(28312..29157)
                     /locus_tag="ACOIAF_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668442.1"
                     /translation="MIYIIEILKISVINIFCFIGIFIVFGLIFSITENLNNKFIFSSF
                     GKTGIIVTGAIGTFVHELSHLIMCLIFMHKINSVKFFRPIESKNDGILGYVSHSYKKD
                     NLYQSIGNFFIGIAPIIGGTIMIILIFKILLPDSYMKVTQNIDLKLYVSMINNLNING
                     FIHIILGDISNFIRIMLLTPSVYSLKYFIFMFLMYSISTHMSLSSADFKNSLNGLSFI
                     VIVIFVISSITYLFGFDNLNISSLIVKYNIFISFFMAIGFIFSIVTLIISYILSLVSP
                     FKNFS"
     gene            complement(29187..29558)
                     /locus_tag="ACOIAF_001427"
     CDS             complement(29187..29558)
                     /locus_tag="ACOIAF_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HGZ2668443.1"
                     /translation="MKLKLLVEEYAVCRLNNDSKIPTWIDTEKFYSITRTDDELSVVC
                     LNNNIPSDVKSEKEWRILKILGPLDFSLVGILSKLSSLLADNKISIFAISTYDTDYIL
                     VKEKDIENACKILSCNGYEVE"
     gene            complement(29711..29971)
                     /locus_tag="ACOIAF_001428"
     CDS             complement(29711..29971)
                     /locus_tag="ACOIAF_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HGZ2668444.1"
                     /translation="MEVLKVSSKSNPNSVAGALAGVLRERGVAEIQAIGAGALNQAIK
                     SIAIARGFVAPSGMDLVCIPAFTDIEIEGDKKTAIKLIIEPR"
     gene            complement(30160..31104)
                     /locus_tag="ACOIAF_001429"
     CDS             complement(30160..31104)
                     /locus_tag="ACOIAF_001429"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HGZ2668445.1"
                     /translation="MIENNSEKIKVLENDIKQLITISKANNIDLSDKINSLNEKLEKL
                     KEDAFSHLSAYEKVTLSRDIKRPTTLEYIEHICSNFLELHGDRLFKDDPSIVGGIGQI
                     GKFNVTIVGHQKGRDTKENIKRNFGMPHPEGYRKALRLMKQAEKFNRPIITFIDTSGA
                     FCGLEAEERGQGEAIARNLLEMSKLSVPVITFVIGEGGSGGALGIGVGNDVCMLEHSV
                     YSVISPEGLSSILFKDSSKAKEACDVMKLTSNDLYDLKIIDKIIKEPLGGAQKDVEAV
                     SKEIKAYILERLNHYKDMDKEEIIAQRYKKFRNIGKCL"
     gene            complement(31125..31979)
                     /gene="accD"
                     /locus_tag="ACOIAF_001430"
     CDS             complement(31125..31979)
                     /gene="accD"
                     /locus_tag="ACOIAF_001430"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HGZ2668446.1"
                     /translation="MIKKFLSSKESKYVTISLDDNFKKNSVDDKFWTYCKGCDSHVFR
                     KDIEENSFVCPKCSRHYGLRARKRINLLIDKGTFMEFNSDVEFQNPLNFPKYKEKVDS
                     YKEKTKESEAVVTGYGRINGIKTVICVMNPDFMMGSMGSIVGEKITYSIEYAAENNLP
                     IIICSASGGARMQEGMVSLMQMAKTSQALSKLEEKSLPYISVLTDPTTGGVTASFAML
                     GDIIISEPNTLIGFAGPRVIEQTINQKLPEGFQTSEFLLEKGFIDMIVDRRKMKEVLY
                     QILAMHKK"
     gene            complement(31994..33355)
                     /gene="accC"
                     /locus_tag="ACOIAF_001431"
     CDS             complement(31994..33355)
                     /gene="accC"
                     /locus_tag="ACOIAF_001431"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HGZ2668447.1"
                     /translation="MIKKILVANRGDIAVRIIRTCKELGIKTVAIYSEIDKDCFHRYI
                     ADESICIGPNNISKSYNNIENIIYLALKLKCDAIHPGFGFLSENPEFAKQCEDNNIIF
                     IGPTREQMILMGDKSRARETMMELNIPVVPGSESVLKTKEEALEVAREIGYPVMIKAS
                     SGGGGKGMRIVRKEEELFSNFDMASSEALAAFSNSDLYMEKFIENPRHIEVQVFGDKH
                     SNAIHLGDRDCSMQRRNQKVIEESLSPYLSDEERQKLHKIAVDIVKGVGYIGAGTIEF
                     IVDKDKNFYFIEMNTRIQVEHPVTEMVTNLDLIKLQISIANGDKIPFKQEDITFRGHA
                     IECRINAEDSSKNFAPSPGKIESLNLPGGFGVRFDTFVYAGYTIPPLYDSMIGKLICW
                     AETREECISRIYRALDEIIVEGINTNVEFQKALVTSEEFREDTHHTKFIEDVFMKKEF
                     ATL"
     gene            complement(33371..33823)
                     /gene="accB"
                     /locus_tag="ACOIAF_001432"
     CDS             complement(33371..33823)
                     /gene="accB"
                     /locus_tag="ACOIAF_001432"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HGZ2668448.1"
                     /translation="MNINEIKELLKAIDSTNLEYVKLESSDLRLEVSKKAQSTSSPVL
                     SVQQESVVDLSLEKPVVNDTPVTSNENLSVVVAPLMGTFYDSPSPDADSFVKVGDVVE
                     EGDTLCILEAMKLMNEITSEIKGEIIEVLVSNEELVEYNQPLFKIKPL"
     gene            complement(34093..34539)
                     /locus_tag="ACOIAF_001433"
     CDS             complement(34093..34539)
                     /locus_tag="ACOIAF_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668449.1"
                     /translation="MESKFFNWYTQSLGGTFGIISCICAYLNGYMFVYTNSASKYFES
                     IGFLGIISSYTLLPLCMLTLIFGVIYSYTPETSDKSIFNIPFFTLNKRLILITVVFGF
                     IGARVYFIIPAILITFNLYSNVLIAKLKYILCKNKENDTNLKESEV"
     gene            34814..35092
                     /locus_tag="ACOIAF_001434"
     CDS             34814..35092
                     /locus_tag="ACOIAF_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668450.1"
                     /translation="MVDTLEFGLKILFFILSIIWMGKIMILRTDKQIVINPLLIGISA
                     VLVMLHTSQSNIEFFGLDVQYIRIVLYIIYSLIILIGIWATNRRNGIF"
     gene            complement(35221..35952)
                     /locus_tag="ACOIAF_001435"
     CDS             complement(35221..35952)
                     /locus_tag="ACOIAF_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668451.1"
                     /translation="MNLANLIPFIISAAALSPINSNDKVLSENQLKDIFPDANLRAVV
                     KRYINPDEMTISNIKALDGEFYATGESISNLKGISYLENVDNFIFWNNNIKEVPKEAL
                     SLKDMDSINLANNYLIDDDVVNSLSHNGVDVNCDLNFIDTKDNQYKLDSKYHNVDIAK
                     GESIDLRKIITKKIDSYYKYWEVTDNLPKDLNFIVSVSDKSVLSCEDMIIKGNKTGQA
                     VVKVILSDKNDLNTSQEVLITVNVK"
     gene            complement(36096..36740)
                     /gene="trmB"
                     /locus_tag="ACOIAF_001436"
     CDS             complement(36096..36740)
                     /gene="trmB"
                     /locus_tag="ACOIAF_001436"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HGZ2668452.1"
                     /translation="MRRRRKKGADEKLLSYTKYVLRDDIDKLKGKWNLKFRNDNPIHV
                     EFGTGKGKFITTLAKQNPDINYIAMELKEEVLLKAVEKADASNLNNILFLWGDVSNIL
                     DYFEAKELSRIYINFCDPWPKNRWSKRRLTHSGFLEMYNRVLEDDGEIHFKTDNEKLF
                     EFSLNEIAANNWLLKNISLDLGNSEYENNVTTEYEDKFMSQGMRIFRCEAKKRN"
     gene            complement(36878..37198)
                     /locus_tag="ACOIAF_001437"
     CDS             complement(36878..37198)
                     /locus_tag="ACOIAF_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein HesB"
                     /protein_id="HGZ2668453.1"
                     /translation="MKITLPETAIDTLKSILKDNQDKPNNIRVYFAGVGCGGPSFGLA
                     LDEKKEDDLTYEVGELQFVMSSDEYSQYGDIIIEDTGFGFRVIPENMKDQGGGGCSGC
                     SGCH"
BASE COUNT        14286 a         4844 c         5921 g        12290 t
ORIGIN      
        1 ggagctccat agataaagtc tatggagctt ttttgcgtat ttaacttaag gaggatagga
       61 ctttgaataa ctttgagatt aaaactagaa taaaatttgg agaaggctca ctaaaagctt
      121 tgcaagaatt aaaaaacaaa aatgtactaa taataacaga cccttttatg gtgaaatcaa
      181 aaacaataga taaaataaca tctaatttaa taaactcaac atatcaagtt tttgaagaaa
      241 tagtaccaga cccaccaata gaagtagtag tagcaggaat agaagcattt aaatctataa
      301 atccagacgt aataatagca ttaggtggtg gttctgcaat agatgcagca aaatctataa
      361 tggacttttc taaaaaaata ttaaaaatta acgaagtaga gtttatagca ataccaacaa
      421 caagtggaac aggttcagaa gtaacatcat ttgctgtaat aacagatagt caaaaaaaat
      481 gtaagtatcc attagtgagt gatgatttac ttccagatat ggcaatacta gacccagagc
      541 ttgtaaaaac tgtaccaaat tttataacag cagatacagg gatggatgta ttgacacatg
      601 ctatagaagc ttacgtatct acaaattcta atgacttttc agatgcacta gctgaaaaag
      661 ctataaagtt agtatttaaa tacttaataa aagcatataa agatggaaat gacatagaag
      721 ctagagaaaa aatgcacaat gcatcttgtt tagcaggtat ggcatttaac caagcatcac
      781 ttggcataaa tcattctatt gcacatgtat taggtggaaa attccatata ccacatggaa
      841 gaacaaactc tatattactt cctcatgtaa tagaatataa ttcatcaata gtaggatata
      901 ctaacacaac ttactctgat acagctaaaa aatatgcaca aatagcagaa ttagtaggac
      961 ttgggagtgc taatataaga atatctgtta gaaatttagt aaatgaaata aagaaactac
     1021 aaaaagaaat gaatatgcca actaaattga cacaatgcaa attaagttta gaagatataa
     1081 ctaatgaaga aagagaaata gcaaaacttg ctataaaaga tacttgtaca ttaactaatc
     1141 ctaaggtacc aactgaagat gatataataa acatattaaa gaacattaaa taactacatt
     1201 ttattacatg taaatataat attatacaaa tagggaaaat gtttttaaaa atataaatta
     1261 tatattgaca aatataaaac ataaaactat aatgaaagta tcacaaaggc gtgatacttt
     1321 agtagaaagc aatggagctt cttaggaata ttaatattat tcttatggag ctccttttat
     1381 tttaatcaaa tagcttttta ataaactata ttcaaagaaa gcagggaata aatttgactg
     1441 aagaatctaa gcaaagagtt attcaagaat atgttcctgg aaaacaggtt acattagcac
     1501 atatcatagc taaccctaat gaagacatat ataagaagtt aggattagta cttgataaaa
     1561 aagatgcaat aggaatactt acaataacac caagtgaagc gtcaataata gcagcagatg
     1621 ttgctacaaa ggcttcaaat gtatctttag gttttataga tagatttagt ggttcagtag
     1681 taatatcagg tgatgtaagt tctgtagaat cagcgttaaa tgatgtttta gaagtcttag
     1741 gaaatatgtt aaacttctca tctacaaaaa tcacaaggac ataataatac aatgaagaat
     1801 ataatattta tgggaaagac aggtagtggt aaaactacct tatgtcaaaa actagaccag
     1861 ttagaattaa agtataaaaa aactcaatca gtggaacttt ataataatgc aatagacact
     1921 cctggtgagt atatggaaaa tagaaaccta tataatgcct taataacaac atcagcagat
     1981 gctaaaatta tagcaatagt gtatgatgcc acacaagaag aaaattatat agcaccaggt
     2041 tttgccagta tgttttgcaa agacgtaata ggaattataa ctaaaataaa taaagctact
     2101 gaaaacgaag tcaatatagc ttatgaaaga ctatcactag caggagcaag tcaaatcttt
     2161 aaaatagaca cagttgataa tattggactt gaaaaattat ttaaatatct agaaaaatat
     2221 aaaaatatag atgaagttta aggggaaata tttttttatt caaaaatatt attaaagtat
     2281 gataaaataa aaaaatgtat aaactatttt aagaagattt aatctttaag cgaggtagga
     2341 acatggggaa aaaaatacta ttggttgatg atgaaccact gttaaggatg gatacaaaag
     2401 atatattaga aagtcatggc tatgaaataa taggcgaagc aagtgatgga tttgaagctg
     2461 ttgaaatgtg taaaaaacac aaacctgatt tagttataat ggatatagag atgccaatac
     2521 ttgatggtat aaaggcaggc aaaataatat gtaaagaaaa tctagctggt ggagtacttt
     2581 tattaacttc atttgaagat aaagaatacg tagaaaaggc taaaagtata ggtgcttatg
     2641 gatatttagt aaaaacagca agtgaaaaat cacttatccc aaccatagaa atgtgtttaa
     2701 ataaggtaaa tgaatttgaa aaaatgaaaa aagatttaga aaaagtaaat tctaaattga
     2761 atgaaagaaa attagtagaa agagcaaaag gtatacttat aagagagttt aagatttctg
     2821 aggatgaagc atacactaga ataagaaaat taagtatgga taaaagaact acaatgatgg
     2881 aaattgccaa aatgattata ataggatacg atgactaata cgataaaaaa aatatcaact
     2941 gcacaaacat acttaaataa taacgaaatt gaaaaaatta tagaagtatc aaaatcctta
     3001 gatttaatgg cttcttttta tgaagctgat gtttttatag atgtactaag taaatttgat
     3061 gatgaagcaa ttgttgtggc acatggtaga cctaaagaaa agtcaatgta ctgtgaaaat
     3121 gttataggta aaaaggcttt aattgaaaat gaaccaggag ttataaagtg cttaaaaaca
     3181 ggagaaagag taagagatat taaagcttta acacaggaat ataaactggt caaacaaaat
     3241 attcagccaa taacactaga tgacaaagtt ttagcagtac taatagttga aaaagatata
     3301 agtagtgaag ttaagaatga gtttaatgct agtgaagacg aagaaaataa aaatttacat
     3361 cagtttataa aacttatagg aaataatgac tttttagttg ataatttaaa ttcagccata
     3421 ttaatatttg ataaaaatgg aaaattaaaa attaaaaatc gtattgcaac tgagatatac
     3481 aaaaatcttg ggtttggata tgatatacaa gatatgcatt acaatgattt agatatagaa
     3541 accaacacct ttgaaaatat attaatggat gaaaattatg atgagacaaa tgaagtttca
     3601 ataggtaata tgttttttaa aataaagacc atatacatca aagatgatga atttaaggtt
     3661 tgcaaaatag tacaagatat aagtgattta aagagaaaag aagaagaatt agttttgaaa
     3721 acagtggcta taagagaagc tcatcataga gtaaaaaata atcttcatac agtaatatct
     3781 ataataaaaa aacaaagtag attatctcaa aatgatgaag taaagcagtg tttagataac
     3841 ataataaata gggtatttgc aatactttcg actcattatt tactatctaa agaagttgat
     3901 aataatatat ctatacgaga aggtatcaat ttattagtgt caaatattca aggtggctat
     3961 ttatttagta aggatataga tatacgtata actggtgaag attttaaaat tagtggagaa
     4021 aaagcaacag caatactttt ggttataaat gaaatactac aaaattgtta tgaccatgca
     4081 ttttcggata gagaacatgg tactatacaa atattaataa ataaagataa cgactataga
     4141 aatattgcta ttatagatga tggaattgga tttgatagta ataatataaa taaaaatagc
     4201 ttagggacat atataataga tagttatata acacagatgt taaaaggaaa tatagagaga
     4261 aaaagtagtg agaatggtac agaagtatta attactatac ctacacaaat taatatttaa
     4321 ataataactc atctacaaag tagtagtttt aattgagcaa agaggcttaa ggttattaat
     4381 agattaagtt aattgaatgt atttataaaa attataagta tatattcaat ttaacaggta
     4441 tttattataa ctttaagcct ctttgcttgt ttaagacata cttaaatttt aggaggtgag
     4501 aaaatgagag aagagttatt aagtgttggt attgacattg gaacaagtac tactcagtta
     4561 attttttcta aattaatagt agaaaatact gaatcaaatt tttcggtacc tagaataagt
     4621 atagtagaca aagaaattat ttataaaagc aaagtttatt tcacccctct tatttcaaat
     4681 aatgaaataa atggtcaaca aataaaagat atagttgaaa atgaatataa aaaagcaaat
     4741 atatctaaag aagatataca tacaggagca gtaataataa ctggtgaaac agctagaaaa
     4801 gaaaatgcaa atgatgtact tcatactttg agtggatttg ctggggactt cgttgttgca
     4861 acagcaggac ctgatttaga aagtataata tcaggaaaag gagctggagc tcatatttat
     4921 tcaaaagaac attcaactac aattgtaaat atagacatag gtgggggaac tagtaacata
     4981 gcctcattta aaaggggaga agtaaccgat actgggtgtt tggatatagg tggaaggctt
     5041 gtaaagatag ataaaataag taaaaaaata acttatatat cacctaaaat agataaaatc
     5101 ataaaagata ataatttaaa tcttcaagta ggtacaatgg ctactgtaga aaatacaaag
     5161 ccaatagtgg atataatggt tgacttatta atggaaagca taggactaag gaaacagaca
     5221 cctttgtttg attttataat aaccaataaa tcaataagta tagatgaaaa tattaaaaat
     5281 gtttcattct ctggtggagt agctgattat atttactatg atggagaaat aaaagattat
     5341 tttgaatatg gtgacttggg aattttactt gggcaagcaa taaaaaattg tgaatacctt
     5401 aaaaaagtaa atgtagttaa atctatggaa acaataagag ccacagtggt tggagcaggt
     5461 tctcatacta ctgaagtaag tggaagtaca ataacttaca ctagcgaaaa gtttccactt
     5521 aagaacttgc cagttttaaa attatcaatt gatgaagaaa caggtacttc aaaacaaatc
     5581 agtgaagcta taaaaaataa acttaattgg tttaaactag aaaatgattt acagaaagtt
     5641 gctatagcca tgataggaaa gaaaaatcca acctttttag atatacaaga gtatgctaaa
     5701 gggtttgtag atggaatgca acaaatcata gaaaatgatt taccttttat agtaattgtt
     5761 gaaaatgata tggcaaaagt gttaggtcaa gcaatatatg cattattaaa ttatgaaaaa
     5821 gaaattgttt gtatagacag tgtcaaggtt gaaaatggag actatatcga cattggaaaa
     5881 cctatagttg aaggtaaagt tttacctgtg gtaataaaaa cattagtttt taattaatag
     5941 aaggaggtaa gtaaatgatt ttaaatacaa aattatttgg acgtacttat cagtttaaag
     6001 gcttatatga tgtaatggct aaagctaatg aagaaaaatc aggagataaa ttagcaggtc
     6061 ttgcagcaga atcagctgaa gaaagaatag cagcaaaagt tgttttatca cacataaaat
     6121 tggaggatat aagaaataat cctgcagtgg catatgaaga agatgaagta acaagaataa
     6181 tacaagatgc tgtaaatgaa aaagtttata aagaaataaa aaattggaca gtctctgaac
     6241 ttagagaatg gctattggat tcaaatacta caagtcttga tataagaaga atatcaagag
     6301 gattaacttc tgaaatgata gcagcagttg caaaattaat gtcaaatatg gacttagttt
     6361 atgcagctaa aaagataaag gttactgctc actgtaatac aactatagga gaacaaggaa
     6421 cattatcagc aagacttcaa cctaatcatc caacagacga cccagatgga atacttgcat
     6481 cacttcttga aggtttaact tttggtatag gagatgcagt acttggatta aatccagttg
     6541 atgactctgt tgagagtgtt actaaagtat taaaaagatt tgaagaagta aaacaaaaat
     6601 atcaaatacc aactcaaaca tgtgtattgg cacacgttac tacacaaatg gaagcaataa
     6661 gaaatggtgc tcctgcagat ttaatattcc aatctatagc aggttctgaa aaaggaaatg
     6721 aagcatttgg atttaatggc gacactatag aagaagctag acagttagcc ttaaaacacg
     6781 gtacagctac aggacctaac gttatgtatt ttgaaactgg acaaggttca gaattatctt
     6841 cagaagcaca tcatggaata gaccaagtta ctatggaagc tagatgctat ggatttgcta
     6901 aaaagtatga accattctta gtaaatacag ttgttgggtt tataggacct gagtacttat
     6961 atgatgctag agaagttgta agagcaggac ttgaagacca ctttatgggt aaactacatg
     7021 gtttaccaat gggtgttgat gtttgttaca ctaaccatat gaaggctgac caaaatgata
     7081 tggaaaactt agccatacta ttaacaacag caggatgtac gtattttatg ggaatacctc
     7141 atggagatga tgttatgctt aactatcaaa caacaggata ccatgaaaca gcatcactta
     7201 gagaaatgtt tggactaaca gcaataaaag aatttgatgc ttggatggaa aaaatgggat
     7261 tctctaaaga tggtaagtta acagagttgg cgggagacgc atctgtatta ttaggatagg
     7321 aggtatttaa tatgaatgag aaggatttaa aagcattggt agaacaatta gttggacaaa
     7381 tggttggaga attagatact aatgttgtgt ctgagactgt taaaaaggca actgaagttg
     7441 tagtagacaa caatgcttgt atagatgata taacagaagt tgatataaga aagcaattac
     7501 tagtaaaaaa tcctaaggat gcagaagctt atttagatat gaaagcaaaa acacctgcaa
     7561 gattaggtat aggaagagct ggtactagat ataaaacaga aacagtttta agatttagag
     7621 cagaccatgc tgctgcacaa gatgctgtat tctcttatgt agatgaagaa tttataaaag
     7681 aaaataatat gtttgcagtg gaaactttat gtaaagataa agatgaatac ttaacaagac
     7741 cagatttggg aagaaaattt tctccagaaa ctataaataa tataaaatct aaatttggta
     7801 caaatcaaaa ggttttaata ttagttggag atgggcttag ctcagctgca atagaggcaa
     7861 atttaaaaga ctgtgtgcca gctataaaac agggtctaaa aatgtatggt atagattcaa
     7921 gtgaaatttt atttgttaaa cattgtagag ttggtgctat ggaccactta ggtgaagaat
     7981 taggttgtga agttatatgt atgttggttg gagaaagacc aggattagtt actgctgaat
     8041 caatgtcagc atatatagct tataagccat atataggaat ggcagaagca aaaagaacag
     8101 ttatatcaaa tattcacaaa ggtggaacaa ctgcagttga agcaggtgct catatagctg
     8161 aacttataaa aactatgtta gataaaaaag cttcaggaat agaccttaaa taaagtcgag
     8221 ggagtgtaga atatgaaaaa tgatatgatt cgtcctaata tactaggaat taaaataatt
     8281 tcaaatataa gtccagaaat ggcacaaaaa ctagaattaa aatcacatca caaaagctta
     8341 ggtttaataa ctgcagactg tgatgatgtt acgtatacag ctcttgacga agcaactaaa
     8401 gcttcagagg ttgatgttgt atatgcaaaa tcaatgtatg caggtgctgc aaatgcttca
     8461 acaaaacttg ctggtgaagt attaggaata atagcaggtc caagcccagc agaagtaaga
     8521 agtggtctaa atgcagtagt tgattttcta gagtatggag caacttttat aagtgcaaat
     8581 gatgatgatt caatagcata ctatgctcac tgcgtatcaa gaacaggtac atacctttca
     8641 gaggtagcag gaataagaga aggtgaagca ttagcttatt tagtagcacc accacttgaa
     8701 gcaatgtatg cactagatgc agcaatgaaa gctgcagatg ttaagatgtg tgaattattt
     8761 gcacctccaa ctgaaacaaa ctttggtgga gcattactta caggttctca atcggcttgt
     8821 aaagcagcat gtgatgcatt tgcagaagca gtaaaatcag ttgcagacaa tccaacagga
     8881 ttttaaggat agttagatgg atgctatagg cttaatagaa gtaataggat atgttgctgc
     8941 aatagaagct tctgatgctt gtttaaaatc tgcaaatgtt aatattgtaa gagttgataa
     9001 ggttggagca ggtattgtta ctttaacaat atgtggagat gtcggtgctg taaaagctgc
     9061 tttagaagca ggtgaaatag cagcttccag agttggtaca ttaagaacat cacatattat
     9121 accgagaatc cataatgaag taacaaatgc tttattcaaa actaaagaac ctaaagtctg
     9181 tgaagtttca gaagataaag aaaaaaactt tgagattaca ttggaaatag ctgataatgt
     9241 atcgaaaaat ttatcagaaa aagaaaatgt aattttagat gtcaatgaaa acagtggaga
     9301 tactgaaaat ttatatagtg atatagataa aacaaacaaa aaaattgaaa cagttgatga
     9361 tagtttaaca gaagctaatg aggaaaaaat agaaaacact aatgataaag aagtattgga
     9421 acaagttaaa atagaaaatt ccgattttga tagagatgac ctatcaaaga acagtgtaaa
     9481 aaaacttaaa gctatggcta agaaattaga ttcttcaata acttataaag acttaaatgt
     9541 tcttaaaaaa gaagaattaa taaacttagt taaaaagctt agcagaaggg atagatagtt
     9601 atgacacttt tagataaaga tttagtatct atacaagaag taagaactct tgtaagaaga
     9661 tctaaagtgg ctcaagagaa actttctaaa atgagtcaag agcaaataga taaaatagta
     9721 aaagctgttg tagatgcagc atttaataat gctactaaat tagctaaaat ggcaaatgaa
     9781 gaaactggat ttggaaaatg ggaagataaa gtaattaaaa atacttttgc atctatggga
     9841 gtttatgaaa ctttaaagaa cacaaaaact attggaataa ttggtgaaga taaagaagcc
     9901 aatacgatag atatagctac accagttgga gttgttgcag gtcttatacc ttcaacaaac
     9961 ccaacatcaa cagtaatata caaaactata atatctctaa aagcaggaaa tactatagtt
    10021 ttctctccac atccaaatgc aaaaaactgc atacttgaaa cagttaatat aataagtgat
    10081 gctgctataa aggctggagc tccagaggat tcaatattat gtataactaa tccaagtata
    10141 gttggaacca atgaattgat gaaacataaa gatacaaact taatactagc aactggagga
    10201 gaagcaatgg ttagagctgc ttactcttca ggaacaccag ctattggagt tggtccagga
    10261 aatggaccag catttataga taaatcagca aatgttaaat tagcaatcaa aagaataata
    10321 gattctaaaa cttttgataa tggtgttata tgtgcatcag aacaatctat agtaatagaa
    10381 aaatctatgg aagaaactgt tttaaaagag cttaaaaatc aaggtgcata tgttttaaat
    10441 gaagaggaaa gtgaaagatt agcaaaattc atattaagag ctaatggtac tatgaatcca
    10501 caaatagttg gtaaaagtgt tgaggtaata gcaaatttag cagacttaac aaatgtacct
    10561 aaaaatacaa aagttctagt tgcaagagaa aataaagttg gtcacaatgt agcctattca
    10621 agagaaaaac ttacaccaat actagcgttc tatgttgaag aaaatgtaga agctattatg
    10681 aacagatgca gagaaatatt gttaaatgaa ggtgcaggtc atacattctc aatgcatgta
    10741 aatgatgaag aattggtaaa aagatttgcc cttaatatgc cagtatcaag aatacttgtg
    10801 aatactcctt cagcacttgg aggaataggt gcatcaacaa accttgtacc agcactaaca
    10861 cttggttgtg gtgcaatagg tggaagttca acttcaaaca atataggacc acttgattta
    10921 ataaatgtta aaagagtagc ttatggaatc aaagaaatag aagaacttgg aagagataaa
    10981 tctcaagttg aaaaatcaat gtttgactca atggataaag aagaattaat aaatcttctt
    11041 gttcaaaaac tagtcaaaga actagcataa ctaaaataaa caatgaaact tatagctatc
    11101 gctataaatt tctaaaaata taaactataa aaaactataa aattaaattt ttggaggata
    11161 agattatggc aagtgcaaac gcattaggaa tgatagaaac taaaggatta gtaggagcaa
    11221 tagaagcagc agatgcaatg gttaaagctg caaatgtaca attagtagga aaagagcaag
    11281 taggtggagg attagttact gtaatggtaa gaggagatgt tggagctgtt aaagctgcta
    11341 cagatgcagg agctgcagct gccgagagag ttggagaact aatatcagtt catgttatac
    11401 caagaccaca ctttgaagta gatgctatat taccaaaggt atcagctgag taatataaga
    11461 ataattataa aatttaaatt ttgattgaaa aagaggctat ctcaaaaatg agtcttacta
    11521 ataaactcaa tgtattttga ggtagtcttt tttatacatc taataggaga tgtgataaaa
    11581 tgggtgtttt aacagaaaac gaagtaaaaa gaaaaattgg aaaaaacaaa aataatataa
    11641 ctgaatttta tatagaaaaa ggtcaaataa taactccttc tgcaaaatct tatttagctg
    11701 agagaagtat tgagcttaaa tatgttgata aactttcaga tgtaaacaat gaacctaaag
    11761 aagttgtaaa agaagtagtt aaagagaaaa aatataaata tactactgta tttggcgcta
    11821 aattggatga aaaaccagaa catatgactc aattaaatgg aaatctttta gtttttaaag
    11881 accataaaag aataatattg agaggaaaaa tagattctct tgaatcagaa atactaaagg
    11941 tacagatact ttgtcagaaa ctagatatgc caaagcttgt taaggaattg caagaagtat
    12001 tagactttgt aagaaatata atgagatgtg aagttttaga tgaaaagcta ggggacttta
    12061 aacttttagg attaactcct caagaactta gagaacaatc tcactatcct aaaaaatact
    12121 ttggtatagg acatgagttc ccaagttatg aaatgggaga agtagtagta gctataaata
    12181 gtataagaag taatacacga gaggttgaac ttgcagccta tgaagcattt aagggtgagt
    12241 atggaaatgt tcacagagaa gatttaataa aggcacttaa tagattatcg tcagtatttt
    12301 ggataatgat atataaggtt agaactaata agtataagta gttggaggac ataatggaga
    12361 atctattaaa tgaaatagtg gaagaagtaa taactagagt taaaaaagaa gcatttatag
    12421 aagttgaagc atcaggaaga cacgttcact taagtagaga agatgtcgat aaattatttg
    12481 gagaaggata tactcttaca aaactaaaag acctatctca accaggtcaa tatgcatgta
    12541 aggaaagagt tacaataact ggtcctaaag gaagtataaa aaatgtagtt gtacttggac
    12601 catgtagaaa tgaaactcag gtagaaatat ctttaacaga tggttcaact cttggactaa
    12661 aagctcctat aaaacaaagt ggagacttag aaggtacttt aagtattaaa atatcaacac
    12721 aatatggtga agttgaactt gataaggggt taatggttgc taaaagacat atacacatga
    12781 ctcctaaaga tgctgaaaaa ttcaatgtat gtgataaaga gatagttgaa gctaaggtga
    12841 tgggtcaaag acctttaaca tttgatgatg tagtaataag agtgagtgac agttttaaaa
    12901 cttatatgca tatagactat gatgaagcca atgcatgtgg ttattcaaaa ggaactgttg
    12961 ctaaaataat aaagaaagca taaatttaac ctactatata aattacctct agggaggatt
    13021 taatatgaat tatgataacc ttgtagattt aataacggaa gaaatctata aaaaaataaa
    13081 tagtaatgaa ataaaaatat ctaataaacc taaagctgtg attgtatttg aacaagataa
    13141 caataaattt aacttattaa aagatgaatt tgaaatagta gaatttgata aaagtataag
    13201 ggaatgtgaa atagtgatag tttcaagact ttgtatgagg gggctatcga atatagcact
    13261 tggaaattca acaagtgatg aagaaagatt tatactaaaa atgattatga agggcaaaaa
    13321 agtatatgtc ctagatgaag gaattgaata caaaaaatat aaggatactg caccaaaagc
    13381 attatataag aaatttatgt catttgaaga tgaaatatgt aaatttggtg tagaaattat
    13441 aaaagactta aatgttataa ctaaaaataa gttaaatata aagaaagaac tagaaagtac
    13501 tagaggttct aaaaaagaag atacaaaagt tttagataaa gatgttttaa atttaaccag
    13561 tgtcgaaagt gtaaaaatag ataatgaatt tagtttagat ttaaggaata aaaaacttat
    13621 atcggaagct gacttaagaa aaccaactat aaatggagtt aaaaacatat tagttaataa
    13681 aaaaagtata ataacaccat tagctgtaga ttttatgaga atacaccatc tcaaactaaa
    13741 gaaactgtaa ggaggtaatt ttatggatat aggaaaagtt gtaggtaatg tttgggctac
    13801 aagaaaagat gaaaaattat gtggccaaaa acttttagtt gtaaaaattt taaaaactaa
    13861 agacacatac aaggaaggct tcatggttgc agccgataat gcaggagcag gaaatggaga
    13921 cttagttctt atatccaaag gttcctcagc tagagagtct attgaaaaat ctcatgttcc
    13981 agtagatgca actatagttg gagtagtcga ttcgtttgag gtgtttgatg aataaaagta
    14041 taggagccat agagtttaaa agcattgcta aaggtataga agtatccaat gaaatgataa
    14101 agaaatcttc agttgatgta ttgtacttaa aaagtatttg cccaggtaaa tttctaataa
    14161 tagtaggtgg agaaacttct tatataaatg aatgtgtaga ttatggaata aaacttggag
    14221 aaggatatat tgtagataac tttgtaataa atgcaatttc acaagaaata ttagatggat
    14281 ttaaaaataa gtatcaaaag ctagatagta tagtctctat aggcgttgtt gaaagtagta
    14341 aagtatgcgc tggaataaaa atgcttgata agactttaaa atctggcgac ttagtattag
    14401 taaaacttca attatctttt gctataggtg gcaaattagt atatatagta gcaggagatt
    14461 taagtagttt agaatatgca ttaaaagaaa gtgaaaatgt agtaagagaa aaagaagtaa
    14521 tatataagac tgtaatacca tcagtagaca gtcaaataat aaaaagtcta ataaaataat
    14581 ggggggaatt atgagtataa acgaaattat aatctatgta atggtagtct tcatggtact
    14641 tggagctata gataaatgta taggtaacaa atttggtctt ggagaacaat ttgaagaagg
    14701 tataatggca atggggtcac ttgcagttgc aatggttggt gttatatgtt tagcaccagt
    14761 tcttgctgat gtattaagac caattgtagt accagtattt aatatacttg gagctgaccc
    14821 agctatgttt gctggaagtt tattagctaa tgatatgggt ggtgcgccac ttgcacttga
    14881 acttgcgaaa gaccctaatg ctgggttatt tggtggatta atagtagggg ctatgatggg
    14941 acctacaata gtatttataa taccagttgc gcttggaata attgaaaaag aagaccaaaa
    15001 attcttagca acaggaatac ttgcaggtat aataacaata cctattggag cattcgttgg
    15061 tggattagtt gcaggattcg aggtaatgat ggttcttaga aacttagtgc caataataat
    15121 atttgcagta gttatagcac ttggattatt aaaattcgaa aatgctatga taaaaggatt
    15181 cacatacttt ggaaaaggtg tagtaatagt tataacttta ggacttgctg ctgccatagt
    15241 tgaagcttta acaggaatag ttgtaatacc tggaatgaaa ccaatcagtg aaggtataga
    15301 aatagttgga gatatagcaa tagttttagc aggagcattc ccattagtgt ttgttataac
    15361 aaaggtattt aataaaccat taatggcttt aggaaaagtt cttggaatga atgatgtatc
    15421 agcagcaggc cttgttgcaa gtttagctaa ctgtatacca atgtttggta tgatgaaaga
    15481 tatggacaat agaggaaaaa taataaatgt agcattctct gtatcagcag cattcgtatt
    15541 tggagaccac ttaggattta cggcaggatt taattctaat atgataacac caatgatagt
    15601 tgctaaatta gttggtggta taacagcagt attgttagct atggttatcg caaataagac
    15661 actaaagaag gagaatgtat aagatggata tatcaaatat agataaaaat ttaattgaaa
    15721 cattagtaag acaaataata gaagaaaaaa taagtggaac taaagacaca gtagattttg
    15781 ttagaaataa agacattagt ggaataactt ctataaagtt accaactgta aaagttagtg
    15841 aatctgatag attagataca gggaatccaa gtgatgtagt ttacacaaaa gatttattta
    15901 cattagagga aagcccaaga ttaggttgtg gtatgatgga aatgaaagaa acaacttttg
    15961 attggactct aaactatgat gaaatagatt atgtaataga tggaacttta gatataataa
    16021 tagatggaag aaaggtttct gcttcttcag gtgaattaat attcatacca aaaggaagta
    16081 agatacaatt ttccgtacct gattatgcta gatttatata tgtaacatat ccagcagatt
    16141 gggcatctca aaattaataa aaatcaagtt aagttaataa taatcaaata aataatttaa
    16201 aatacaaact cttaaataag cacacatatt taaaaaagct atgaattaaa attcatagct
    16261 ttttttataa agaatgttat attttatact tattttttct atatttactg ggagacatat
    16321 tatatttttt attaaataat ctaataaaat gctctacatt tggataacca atactatttt
    16381 actgataatc ttttccttat cactaatctt acattttaaa atattatttt tactcgtaat
    16441 attatcataa gaatttgttt cttatttaaa actaattaga attataaagt ttattgccat
    16501 gttattaata aaataaatcc agttaaatcg agtgctagtt aaagtaataa gaaactttta
    16561 caatggtttc ctttatcgat tgaaataaaa gttaatatgg tataatatct aaagagagaa
    16621 tcttatcaat tagaatatgc atagaaacaa gtggcttaaa aggagacgtt atatggaaga
    16681 gatagtaaag aaggaacaag caagtatgaa tgatattttt ttgtatctta aagctaaatc
    16741 aaactcatta tataaatttg taatattata taatgagttt atcaataaac ctaaagatta
    16801 tggaactggg gaacttataa acatggttga agtacatatg ctaacagcga ttgaagaaaa
    16861 tccaggaata agtccaactc agttagctaa aatatggaac agaactaaag gtgcaatatc
    16921 tcaaactata tctaaattgg agaaaaaggg ttatataact aaagaaaaga aagaaggaaa
    16981 tgcaaaaact atattactat atccaaccac atcaggagta gagttaagta aggcacataa
    17041 actatatgac accatagatg ttacaagaac atttgaatac ttaatcaagg agtgtacatt
    17101 agaggagata gatagttttt ataaagtaat tgatttgttt attaatttat tagaagaatc
    17161 tccgcaggaa aataatgatt ctgaaaaagt gtagctaaga ttgaagttga ataaagagaa
    17221 atagatatta tagaagtagt acttaagtac acattatata aaaatataat aggtatagga
    17281 ttaaaaagtt tagtggttta aagtttaacc actaaacttt tttaatgtct ataatctact
    17341 attatttaat ttactaaaaa taaaaaaact cattatatta agataaaaat agaaaaataa
    17401 ctagaaaaat actataatat atcagcttaa tacagaaaaa agttaaaaaa tttgagaaaa
    17461 ataaaaatga aaacgtttta tattgacata gagaaaaagt agtgatacca tttaaatata
    17521 gtttagtagc taaactatat ttaaattata ataactattt tagggggatg ttatgaattt
    17581 aaaacaatta agtgaaaaat acgaagaata tgtaattcaa atgagaagag aatttcataa
    17641 gtatccagaa ttatctttta aagaaataag aacaacccaa agaataaggg aagaattaca
    17701 aaaaataggg gttccttttg tatctattga accaaatatc gtaatagcta ctatacaagg
    17761 caaaaatgaa ggtaagacaa ttgcaattag aggagatata gatgcgcttc caatgcaaga
    17821 agattcagat gttgaataca aatcagaata tgatggaata atgcattctt gtggacatga
    17881 tgctcatgga gctatgttac taggacttgc acatatgcta aatgatataa gaaaagattt
    17941 aaaaggtaga gtattattag tttttcaagc agctgaagaa gttggtggtg gacacaagga
    18001 gataatacag tactttaagg aaaatggagg accagataga ttaatagcta ctcatgtttg
    18061 gtccgatatt gaagctggta aaatttctgt tgaaccagga cctcgtatgg ctggtgggtc
    18121 tggttggaga atagacataa caggtagagg aggtcatggt tctagaccag atcaaagcat
    18181 agatcctata aagccagctt gtgatatagt tttaaaagta tcttctatac cagttaatca
    18241 tataagtgta ctagaacaat gtgttgtaca tgcatgtgca atacatggag gaactacaat
    18301 aggaaatgtt attccaaaca atgcagaggt aataggtgga tatagatatt ttactgaaga
    18361 atcaggcaaa aaagtgtttg acattataaa gcaaatgagt aatggtgttg gacaagttta
    18421 tggagtggat gtgaaggtta ctcatactgg aggcatagga cctgttatca atgatgaaga
    18481 agcagttaag atggctaaag aaatagtttc taatatagat ggattggagt tagattctta
    18541 tgagccaata tgtgcatcag attgttatgg tgaaatatta aaagagtatt ctggctttta
    18601 ttgttatcta ggggtaggta acaaagaaaa aggaataata tatgcacaac accatcctaa
    18661 gtacaatata gatgaagata cattaaagtt aggttgcgaa tttatggcta aatatgcagt
    18721 tgaattttta aataaaaata ataactaaaa taaatcgaat tttataaatg gggggatgaa
    18781 aatgcaaggt gcattaaagg aaaataagaa aaaaagtggt ggaggatttt taggatggat
    18841 tgaaaaagtt gggaataaaa tgccacatcc tctagcatta tttacatata ttacactgat
    18901 agtagtagca ttatctgcaa tagcaagctt tttaggtttt tcagcaacaa gtcctgctac
    18961 aggaaagtta ataaaagtta tgaacttaat aagtgcaaaa ggtttagtac tatttatgca
    19021 agagtttgtt aagaacttcc aaaacttccc tgtgcttgga gtagttttag ttatggcagt
    19081 tgccacagga gtttgtgaaa agacaggatt tttttcaact gccataaaaa tgagtttatc
    19141 aaaagttaag gggaatgcgg ttgtatttat aatagctttt ataggagtat ttgctaatca
    19201 agctggtgat gctgcttttg tattagtacc aactcttgca ggagcaatat tttatggatt
    19261 aaatagaaat ccattagctg gtatattctg tggatttgca tcagttggtg gtggatttgc
    19321 aacagctgta attcctgggg gatgggacgt tacgctaacg ccaataactg tatcagctgc
    19381 tcaaactatt caacctgcat ttgatatgac attattagct agttattatg cactatttgt
    19441 atctgcgttt atagtagcta cagtatcaac aatagtaacg gttaaattta tagaacctaa
    19501 attaggtaag tatactggta aaccagaagg tatagatgat aatcaaggtt ttgaggtcac
    19561 aaaagaacaa gcaaaagcag caaagttagc aggttttaca gttttagctt atgtagcgtt
    19621 attaattgct ttgtgtatac cagctaatag ttttttaaga agtccagaag gttctttaat
    19681 atttggagca ccacttatgt cttgcttaca gttttttata gtaatactat tcttcttgcc
    19741 agggattgtg tatggaatga gggttggtaa aatcaagact gtaaaagact taaatgatat
    19801 attatgtcaa tcaatggcag caatggcacc gtttatcgta cttgcaatag ttataggtca
    19861 attcttaacg ctattctcag tgtctaattt agcacaggta ctagctataa aaggtggagc
    19921 attactcaac tcattgccac taccaccaca agctataata ctgctattct tagtactagt
    19981 agcgtttata aatatatttg ttataagcgg gagtacaaaa tggatgatat ttggaccagt
    20041 atttgttcca atgttaatgc aattaaacat acatccagca tttactcagt ttgtatatag
    20101 attaggagat tctgtaacca atcatttatc accattaaat gcattcttta taatactttt
    20161 agcaacagtc caaaaatacg ataaaaatgc aggtatggga actatattct cagctatgat
    20221 accttacact ataggttact ttgtagtact tgcagtttta gtagtagttt ggatgacaat
    20281 aggtatacca gtaggaatag gtggacaagt ttggttataa aacatattta ataattttgg
    20341 gaggaaagaa aatggaaaca actaaaataa gatacaattt tgatgaagta atagatagaa
    20401 gtggaacaaa ctgtcttaaa tatgatctta agagtcaatt tatgccagaa tctccagaag
    20461 atgcactacc actatgggta gcagatatgg attttgcatg tgcccctgag ataattaatg
    20521 caatgcatga aagaatagac agaaaaatat ttggatatag ctctcaacaa actgatgatt
    20581 attatgaagc tgtatgtgga tggtttaaga gaagatttga ttggaaaata gaaaaagaga
    20641 atatattctt tagcccaggg gttgtgcctg cgttaggatt tttcacacaa atattaacac
    20701 aaaagggtga tggtatcata atagaaaagc ctgtatacta tccttttgaa gataaaataa
    20761 ctaacaatga aagaaaagta gttaatagtc ctttaaagtt tgaagatggt cattattcta
    20821 tggattatga tggatttgaa gaattagcta aagatgaaaa taataaggtg tttatattat
    20881 gtagtcctca taatccagtt ggtagagtat ggaaaaaaga agagcttaga aaaatagtag
    20941 acatatgtaa gaaatatgat ttgtggataa tatctgatga aatacattgt gatattattc
    21001 gtaaaggtga aaagcatata ccattagaag tagcatgtcc tgattataaa gataaaataa
    21061 taacttgtac agctccaagt aagagtttta acttagctgg tatgcaatta tcaaatatag
    21121 taatcaataa taaggaatta caagaaaaat ggatgcttga aacagattct aaattagcta
    21181 tattcctacc taatccattc gcaatagttg ctactaaagt tgcttataat gattgtgaag
    21241 attggatgaa tcaagtaaat gagtatatag atggtaatat taaatatgta gaagattttg
    21301 ttaatgagta tatgcctaaa gtaaagataa taaaatctca aggaacttac ttagtttggt
    21361 tagactttag agggtatgga ttagatgcaa aacagcttga atatgtaatg cttaacaagg
    21421 cgaaggtatt gctagatgaa ggatacatat tcggagaaga aggagcagga tttgaaagaa
    21481 taaatgttgc ttcacctaga acaattatag aagagtgtat gaatagaata aaatgtgcat
    21541 ttgaaggatt ataatagtaa tatctaaata atatatttgt gtattttaaa aaaagactat
    21601 gctaaaaaaa tatttcaaag catagtcttt taaagtttta tgatgagttt aaaaaatttc
    21661 aaaacgttac tattcactta tatgtttata ttagaaataa ttccacaata tactcatttt
    21721 ctaaagttgc acttttatac accttgcttc accaatatca tgtgtaacca aaatagtaga
    21781 cttattctca ttcttaataa tactataaac atcatcacaa actaataatc tagtctgata
    21841 atcaagagct gaaaaaggtt catctaaaag tagaatatct ggatttacag aaagtgttct
    21901 aataagcgca actctttgtc tcatacctcc agataattct tttggataca tacttctaaa
    21961 atcccataat ccataagtct ttaaaaggct ttcaactcta tctaaagatt ttttatcttt
    22021 tttgccttga atttccaatc caatagttat attatccatt atatttctcc attcaagcaa
    22081 attatctttt tgaaacatat atccaatatt tccagtaatc tttatatcac cactagtcgg
    22141 ttttaaaaga ttagtaagaa tatttaatat tgtagattta ccacttccag aaggaccaag
    22201 caaagttaat atttctcctt ctttcaagtt aaaattgaca tcctttaata cttcaagctc
    22261 gccttcctta gtgtagaagt tcatatttat atttttaact tctactatgt tgtcattcat
    22321 tatgccaccc cctataactt ttagctcgta tatattatgt tattaacaat atgacctatt
    22381 tgtaccattt ttcataaata taatataaat attttgttta tttacgtatt ttcttagtaa
    22441 cttgtacgta taaattatag ataaataaat tataaggtag gggataaatt tgagtaacat
    22501 aataacgaat ttaatagagt caatatttac taatccaata actatattgt ttttacttgc
    22561 aataactgtg tattctgttt ttaaaataat agaaaataac agagtatatt catttgtaag
    22621 ttcaaaatta gacgaaatca atagagacta taagacaagt gagttttata aaaaggtaag
    22681 ggatgactac tatacatata gtaaagaaaa tccttatgca gacgtaaata taacttcatt
    22741 tatagaggaa gttgtatctg atttaaagca taataatttg ccattgttag aaaaaattag
    22801 aagcataaaa aattcatctt caataagtat attacttggg gttttaggta cgtttgttgg
    22861 tctttctaca atgcttttat gtgttgatac taaagacatt ataaattcac ttccttcaac
    22921 tattagtagt atgcaaactg catttacaac tagtatattt ggagttgtat tttctctaat
    22981 tataggttat tttactaaga taaaagattg tgaacatgtt ttgatacaaa taatgttaaa
    23041 atctgaaaat ttactaacat ctgaaataac tcattttaag tcagaaagaa tggatttaaa
    23101 agtagaagag gtaaaaaata caataaaaca aataagtaag tctatagaag ctattgaaaa
    23161 atttgataaa atatctaaag atttaaatga ctttaatgat gagtttatca gcggtataga
    23221 agctttaaaa agccttttag agggttctca aagttctatt aagacatttg accagagcgt
    23281 aagaaaatta gataaacagt ttaatatttt aaatataaaa tttgtaaagc tttttgataa
    23341 atatgataat caagataata taaataaaga gattttattc gatataaaag aatcttcaaa
    23401 aaatatatac aatgccactg aaagccaatt taaaattaga gattatataa aaaatataaa
    23461 tgcaggattt gctttatatg aaagaagtgc tcaagattta ttaactaaat taatgaccca
    23521 tgaaaataaa atatctcaaa atcaaaaaat ccttttagat gaaaagttta ccttagatga
    23581 ctcaataaaa aatctctcaa gtattattga aaacttttct aatgacctac aagttaaact
    23641 ggatatgatg tttgaaaatt ctttagatat tcaagataag ttagatgtta tgtttaataa
    23701 ctcttttatg aatgatgaag ttcctcttga tagtgaagaa ttatttaagg atgatgttaa
    23761 taatgtattt aatcctttta gtgaagaaat ttatgagata gaggataaag aaataaaggt
    23821 cataggtgag gatgagttaa atgaataata aatatagaag aactgttaac agagaatttg
    23881 aaaagagtag cttttggcca acatttacag acttattatc tactgtattg atggttgtga
    23941 ttcttatctt atttagctca gaaagtattt caggttctgt tgaacaagat ttagctaaga
    24001 atgtcaatgc ttctgttgaa gaaactttta aaaaaagcaa aatgcctgtg aaggtagata
    24061 aatctaatgg gcaagtaact tttggtgaaa gaaccatgtt tgatgtagat agtgatgttt
    24121 taaaaccaga ggctaaagaa atgctaaaaa tgtttgttcc aaagtacata gagactattt
    24181 ataaagatta tggtgactat atatccaaaa tagttataga aggtcataca gatgatgttg
    24241 gaagttatat atacaactta gatttatcgc aaagaagagc atatagcgtt gctaaattta
    24301 tagtgggaga tgaaataggt gactataaat acaaagataa agtgactaaa catattattg
    24361 caataggaag gtcaaaagct gaactcatca aaaatagtga caatagcgtg aatagagatg
    24421 catctagaag agtagaacta aaatacgaaa taaatattaa tcaaaataaa taattaaatt
    24481 tttataaaaa aaaagaaggc agttggtgtg agctttaagc cttctttaag gaaaaagttt
    24541 atttattttt gttgatactt aatatattat agtttttaga agcagtttgc aatatattaa
    24601 acgtaaatgg tcgtttaaaa tgcataactg gatactactg atgtaaaata tttcatttta
    24661 tataatataa aaattataca gtgtcaattt tctatgttat tgtactataa tctattattt
    24721 aaaatattat gtaaaaaaat atcataaaaa cgcatatcat gttataatta ctagagtatt
    24781 atgaatatgg aggcgtagtt tgtgatagaa ataagtataa aaaataaaaa aaattctaaa
    24841 attaaaactt tgatactttt attgatagta tctatatcga tttatttaac tggttgtgct
    24901 acatcagata atggaaataa tcaatcaaat aatgatgatt ctttatctag tagtatatca
    24961 agtgataaga atgtaaaaat tcattttatt aatactggaa acagtgatgc aatattgatt
    25021 caagatggta aaacttttac attgatagat gggggagata atgatgatga gaaattaatg
    25081 gtagattacc taaataatca aggtgtaaaa gatattaaat atcttatagc tactcattct
    25141 catgcagacc atttgggtgg acttgattca gttgtcaaaa acttcaatat agaaaatgtt
    25201 tttgtatcga atggttcagc agaaacaaag agttataggg actttataaa tgctttagca
    25261 aataaggatt tatctccaag tgtaccactt gaaaataata agttctattt agaagattct
    25321 tactttgaag ttctaaatac aaatggagga gatagtacta atgaacaatc tctagtcctt
    25381 gtatatacta atgggaatga taaagtttta tttacaggtg atgctgaaga aggtaccgaa
    25441 aaagaaatac tacctaagct tgagaaagta gaccttttaa aagtagctca tcatggttct
    25501 cgtagtagtt catctcaaga gtttttggat aaaataaatc ctgaatatgc ggttctttta
    25561 gtaggaaagg gtaattcata tggtcatcca catcaggaga caatgaataa attaaaaaaa
    25621 atgggagtaa aagtacatag aagtgatgaa tgttcagata taatatttga atcaacaggt
    25681 gatggcgtat ttacaagttg taaagatgga agttataata aaggcgttag agaagatgga
    25741 aattctggaa aatcaaacag taatacaagt aaaaataatt cttttaaaaa taaacaaaat
    25801 acatccaact cagaagaaat agtgtatttt actccaaaag gtaagagtta tcactctaca
    25861 aaaaattgta gtgggctttc aagaagtaaa aaaatattat caggcacaat tgcagaaagt
    25921 aagaaaaatg acccatgtga tatatgttat ggaaaataac aaaaaagcaa ccaatttatc
    25981 tgtaaagctt ctaagcttta aatcatttaa aaagctttaa atcatttaaa aaataaaata
    26041 aattcataaa aaaataagct ataatcaata gataacacat tttatctata cgaatagctt
    26101 atttttttaa gtatttttta atagctattt aataactcta aatacaccag ttacaatacc
    26161 tacaatttca acttctgagt cattgagcat aattggttcc ataaaatcat tttctggctg
    26221 aagtcttatt aaatttcctt ctttaaaaaa cctcttaact gtagcagatt caccatttat
    26281 aagtgcaact actatctgag aatttaaggc tgtatttttt ttgtctacta caacgtaatc
    26341 ttcatctaaa attcctgcat taatcatact ttcgcctttt actcttaata caaaattgtt
    26401 actgcccttt actaaactgg cagggaatgg aatgtattcc tctatgtttt gttgagctaa
    26461 aatgggttct ccagctgtta tttgacctac taaaggaagc tctattattt cttgattagc
    26521 tccagtaaca tcatttactt tactacgttc taaaacttca atagctcttg gtttagtagg
    26581 gtcctttctt atgtatccca gtttttcaag tttatttaga tgagagtgca cagttgaagt
    26641 agatcttaac cctacagcag tacatatttc tcttacagca ggtggataac cttttaatgt
    26701 gatttgagac tttataaatt ccaatatcaa gacttgcttt tcagttagat ctaaatacat
    26761 aacatcactc atttccaaac atattttgtt tttatttcca tacactttat atttttaatt
    26821 ataacataat ccatttatat ctgcaaacat ttgttcctga tttttaatcg tcctcaatcc
    26881 attcaaaatt cttgactata tcacgtttta catttttctt atgagctgca taaagctgag
    26941 tagttgtgac attatcatgg tcaagtaagt tttgaacatc atatatagaa aatcctgttt
    27001 ggattagaga agtcgcagcc gttgccctta atttatgggg actatatcca ttgtctctag
    27061 tagtatcaag tggaatagat gtatattttt ttaccagtgt tcttatagcc tttgctgtaa
    27121 ttctcttatt ttgaagagac aaaaataaag catctttttc ttcatcattt aatagttcat
    27181 ctcttgttct ttcgttttgc aaataatctt ttattacatg ctcacaagta tgatttatag
    27241 gcattaaaac ttctttacca cgttttctat atattttaaa ttcacctctt gaaaaattaa
    27301 aagaggagat attgagttct ctaagctcat ttaatctaag cccgtatgta acaaacaatg
    27361 ctagtatagc tttatcacgt aacttagtct ttctccaata taccttttct ttctctgtaa
    27421 gcccttcacc tgtttctact gattcaagca tcttagcaac ttcatctatt tcaagacgct
    27481 taatagcatc tggctgaggt ttaggaagtt ttattggatt gaatccatca gttatattat
    27541 tatctatctg agagtttcta tataagaatt taaatagagt agagatggat gatttttttc
    27601 ttgctaaagc tctattgtta ttttcaaaaa tcaatgtatt tttttctgta tttttataat
    27661 atctactaca ataatcacct aagaataaat ttatatctct tgattttatt gtattaaaat
    27721 catcttcttt aatatctttt acacaatctg catttgttaa ttctttggtt tcaatcaaat
    27781 atgaacagaa aaaattaata tcttctaaat aagcaagtcg tgtagaaact gcaacagaac
    27841 cttttagata tataaaataa tccttcataa aagcaggaag tttattttcc aaaagtagac
    27901 atttttctat gagttcattt tctttgatga caatattatc aggtttatct gatttattat
    27961 gtacttttat aattttctta tcattattca aaattaaacc tcctaaaact aaaatataaa
    28021 tcttttatat tattttttga tatttatacc aagtgagtat aaatatttaa tttaatcatt
    28081 ttttgtattt ttaagagatt agcagtgctt tagatgaaat tgccagtaac tattccctaa
    28141 atccacattt aaagactagt tgaatttaaa tactcttttt tatttctcaa tatatttata
    28201 gaaatatttt aaaatatatt gagaaatttt ttatgctcta ctaaaatctt ttttatataa
    28261 tatttttata tgtttctaac gaaaccaaat attagtaaaa tatgtattta tttaactaaa
    28321 gtttttaaat gggctgacta agcttaaaat ataggatatt atgagagtta ctattgaaaa
    28381 aataaaccct attgccataa agaaacttat aaatatgtta tacttaacta ttaaactgga
    28441 tatattcaag ttatcaaatc caaataagta tgtaattgaa cttattacaa aaataactat
    28501 aactataaag ctcagaccat ttaagctatt tttaaaatca gcactactaa gtgacatatg
    28561 tgtagatata gaatacatta aaaacataaa tataaaatac ttcaatgaat atacactagg
    28621 tgttaacaac attattctta taaaattgga aatatcacct aaaataatgt gtataaaccc
    28681 atttatattt aaattattaa tcatacttac atataacttt aaatctatat tttgagtcac
    28741 tttcatataa ctatctggca gtaatatctt aaaaattaaa ataatcatta ttgttccacc
    28801 tataataggt gcaattccta taaagaaatt tcctatactt tgataaagat tatctttttt
    28861 atagctatga cttacatatc ctagtatacc atcattttta ctttctattg gtctgaaaaa
    28921 cttaactgaa tttattttat gcatgaaaat taaacacatt atcaaatgac tcaattcatg
    28981 aacaaacgtt cctatagctc cagttacaat gattccagtt tttccaaaag atgaaaatat
    29041 aaatttatta tttagatttt ctgtaatgct aaaaatcaat ccaaatacaa taaatattcc
    29101 tatgaaacaa aaaatattta ttacacttat ttttaaaatt tcgattatat aaatcataag
    29161 ttaagtcctc atgtaaaagt atttaactac tctacttcat atccattaca gcttaaaatt
    29221 ttacaagcat tttctatatc tttttctttt actaagatat agtctgtatc ataagtagaa
    29281 attgcaaata tgcttatttt gttgtctgct aacaaactac ttaacttaga taaaattcct
    29341 acaagtgaaa agtctaaagg acctagtatc ttaagtattc tccactcctt ttctgactta
    29401 acatcagaag gaatattatt atttaaacat acaacactta attcatcatc agttctagtt
    29461 atagagtaaa acttctctgt atctatccat gttggtattt tactgtcatt atttaatcta
    29521 catactgcat actcttctac caataatttt aatttcataa tgtatatttc ctccaatctt
    29581 atttgtttac ttaagattat acattaaaag ttacataaaa taaaacatat aaagtaaaaa
    29641 aggttcacta tagtgtccta tttctatagc caacctttaa aacttaatta aattctaata
    29701 aagagatata ttatctaggt tcaattataa gtttaatggc tgttttctta tcaccttcga
    29761 tttctatatc agtaaatgcg ggaatgcaaa ctaaatccat tccactaggt gcaacaaaac
    29821 ctcttgctat tgcaattgat tttatcgctt gatttagagc tccagctcct atagcttgta
    29881 tttctgcaac gcctcgttct cttagtactc cagccaaggc tccagctaca gaattaggat
    29941 tagattttga tgaaactttt agtacctcca tttcaacatc tccttgttaa tttgttaaag
    30001 tatccattac ctaaagtact aaatcataat tgccatttac taattacaat catcatttaa
    30061 agtatacttt tataaaaata tacttctata atcgttttaa gaaaaccttt atttttttta
    30121 accattttat tatgaaaaaa ctacaaattt ctttatattc tataagcatt ttccaatatt
    30181 tctaaatttt ttatatcttt gagctataat ttcctcttta tccatatctt tgtaatgatt
    30241 taatctttct aaaatataag cttttatttc ttttgaaaca gcttctacat ctttttgtgc
    30301 tcctccaaga ggttctttta taatcttatc tattattttt aaatcatata aatcattact
    30361 tgtaagcttc ataacatcac aagcttcttt agctttagat gaatctttaa atagaatact
    30421 agaaagccct tctggagaaa tcacagaata aactgaatgt tctaacatac atacatcatt
    30481 tcctactccg atacctaaag ctcctccact accaccttca ccaattacaa aagtgattac
    30541 tggaacagaa agtttactca tttcaagtaa gtttcttgca atagcttctc cttgtcctct
    30601 ttcttctgct tctagaccac aaaaagctcc tgatgtatca ataaacgtta ttataggtct
    30661 attaaatttt tcagcttgtt tcataagtct taatgctttt ctgtatcctt ctggatgagg
    30721 cattccaaaa tttcttttta tattttcttt tgtatctctt cctttttggt gtccaactat
    30781 agtaacatta aactttccta tctgaccaat tcctccaact atagaagggt catctttaaa
    30841 aagcctatct ccatgaagtt ctaaaaaatt agaacaaata tgttctatgt attccaatgt
    30901 cgtaggtctc tttatatctc tacttaatgt taccttttca tatgctgata gatgtgaaaa
    30961 agcatcttcc ttaagctttt ccaatttctc attaagtgaa tttattttat cacttaaatc
    31021 tatattattt gccttagaaa tagttattaa ttgtttaata tcattttcta agacttttat
    31081 tttttcacta ttattttcaa tcaaaacagt tcctccttta tgccctattt cttgtgcata
    31141 gcaagaattt gatataatac ttccttcatt tttcttctat ctactatcat gtcaataaaa
    31201 cctttttcaa gtaaaaactc agaagtttga aatccttcag gaagtttttg atttattgtt
    31261 tgctctatta ctcttggacc tgcaaaacct atcaacgtat ttggctcaga aattataata
    31321 tcaccaagca tagcaaaact agcagttact cctccagtag tagggtcagt tagaactgat
    31381 atatatggca atgatttttc ttcaagctta gaaagtgctt gagaagtctt tgccatctgc
    31441 ataagagata ccataccttc ttgcattcta gctcctcctg aagcagaaca aataattata
    31501 ggtaaattat tttcagcagc atactctatt gaataagtta ttttttcccc aacaatagaa
    31561 cccatacttc ccatcataaa atcaggattc attacacaaa taactgtttt aattccattt
    31621 attcttccat aacctgtgac aacagcttct gactcttttg ttttttcctt ataagagtct
    31681 actttttctt tgtatttagg aaaatttagt ggattttgaa attctacatc agaattaaac
    31741 tccataaaag ttcctttatc aatcaataaa tttattcttt ttcttgctct taagccataa
    31801 tgtcttgaac attttggaca tacaaatgaa ttttcttcta tgtcctttct aaatacatga
    31861 ctatcacaac ctttgcaata ggtccaaaat ttatcatcta cactattctt tttaaagttg
    31921 tcatctaaag aaatcgtaac atacttagat tcttttgatg ataaaaattt ttttatcata
    31981 atctatttac tccttataat gtagcaaatt ctttcttcat aaagacatct tctataaatt
    32041 tggtatgatg agtatcttct ctaaactcct cacttgttac aagtgccttt tgaaattcta
    32101 catttgtatt aataccttct acaataattt cgtctagagc tctatatatt ctacttatac
    32161 attcttctct agtttcagcc caacatatta acttacctat catagaatca tataaaggtg
    32221 gtatagtata tcctgcatat acaaaagtat caaatctaac accaaaacct ccaggtaaat
    32281 ttaatgattc tatcttacct ggtgatggag caaaattctt tgatgaatct tctgcattta
    32341 ttctacattc aatagcatga cctctaaaag taatatcttc ttgtttaaaa ggaattttat
    32401 ccccattagc tatagatatt tgaagtttta taaggtcaag atttgttacc atctctgtaa
    32461 ctggatgttc aacttgtatt cttgtattca tctctataaa ataaaagttt ttatctttgt
    32521 caactataaa ctcaatagta cctgcaccga tataacctac tccttttact atatctactg
    32581 caattttatg aagtttttgt ctttcttcat ctgaaaggta tggacttaaa gattcttcta
    32641 ttactttttg atttcgcctt tgcatagaac aatctctatc acctaaatgt attgcatttg
    32701 aatgtttatc tccaaatact tgtacttcta tatgtctagg attttctata aacttttcca
    32761 tatataaatc actattagaa aatgctgcta gagcttctga acttgccata tcaaaatttg
    32821 aaaaaagttc ttcttctttt ctaacgattc tcataccttt tccaccgcct ccacttgagg
    32881 cttttatcat aacaggatat ccaatttctc ttgcaacttc taaagcttct tccttagttt
    32941 tcaaaacaga ttctgaacca ggaacaacag ggatattcaa ttccatcata gtttctcttg
    33001 ctctagattt atctcccatc aaaatcattt gttctcttgt tggacctata aaaataatat
    33061 tattatcttc acattgcttt gcaaattcag gattctctga taaaaatcca aagcctggat
    33121 gaatagcatc acactttaat ttaagagcaa gatagattat attttctata ttattataac
    33181 tcttacttat attatttggt cctatacaaa tagattcatc agctatatat ctatgaaaac
    33241 aatctttatc tatttcagaa tagatagcaa cagtttttat acctagttct ttacatgttc
    33301 tgatgattct aactgctata tcgcctctat ttgcaactag tatttttttt atcaacttaa
    33361 atacctccta ctacaatggt tttatcttaa ataaaggttg attatattct actagttctt
    33421 cattactaac taatacttca attatttcac ctttaatttc acttgttatc tcattcataa
    33481 gcttcattgc ttctaatata cataaagtgt cgccttcttc aactacatcg ccaactttaa
    33541 caaagctatc tgcatctgga cttggagaat catagaatgt tcccatcaaa ggagctacta
    33601 ctactgatag attctcatta gatgtgacag gtgtatcgtt tacaactggc ttttctaaag
    33661 aaagatccac tactgattct tgttgtacac ttaaaacagg tgaacttgta gattgagctt
    33721 ttttagatac ttctaatctt aaatcgctac tttcaagttt tacatattct aagttagttg
    33781 agtctatggc ctttaataat tccttaattt cattgatgtt cattttgtta cccccttgta
    33841 ttattacctg gtgctaatat tatatcacaa aaaacaaatt tataaaagaa aaagtgctga
    33901 ctagagaatc aacacatcaa tatatctttt aattttaaat taacaaccag taacttatat
    33961 ttgttttaat tttccattat taatataaat tataagtata aaatgaataa aattcaatat
    34021 tttttgcaaa atacaagaga atacaaatta atacctcggg aatttttaag acaaatacca
    34081 atattatgca atttagactt cgctttcttt caaattagta tcattttctt tatttttaca
    34141 taggatgtac tttaatttag ctatcaaaac attgctatat aggttaaaag ttattagaat
    34201 tgcaggaatt ataaagtaaa ctctagcacc tataaaacca aaaactactg taataagtat
    34261 taatctttta tttaaagtaa aaaaaggtat attaaaaata cttttgtcgg atgtttctgg
    34321 agtataagaa tatataactc caaaaatcaa agtaagcata caaagtggaa gtagtgtata
    34381 acttgaaatt attcccagaa atcctatact ttcaaaatat ttgctagcac tatttgtata
    34441 aacaaacatg tatccattta aatatgcgca tatacatgat ataatgccaa aagttccccc
    34501 taaggattga gtgtaccaat taaaaaattt agattccata tacctcacct cctaaaactg
    34561 tatatataat gatatgcatt taaatagtaa aatatatctt tatatgtttc tgaataaaat
    34621 tttgttaatt tcattaattt atgtttaaga aatttgaagt atagtgtagt atatattaat
    34681 gtagatgaag ttttatagta cttatttcat agtgcttaat caagttatct tattatggat
    34741 tcaaaagttg tttgtagggt tttatatttt gttgagctga aaaatttatt ataaatgaag
    34801 ggatgttttc gctttggtag atacattaga atttggtctt aaaattttat tttttatatt
    34861 atcaataatt tggatgggta aaataatgat tttaagaact gataagcaaa tagtaatcaa
    34921 tcctttgctt attggtatat ctgcagtcct tgtgatgttg catacctcac agagtaacat
    34981 tgaatttttt ggattagatg tccaatatat aagaatagtt ttgtatataa tatattcact
    35041 gataatttta ataggaatat gggctactaa cagaagaaat ggaatttttt agataatcta
    35101 tttctataaa atttaaaggt tgctagtaac aaatcactaa tattagcgtt ctgttactaa
    35161 caacctttat tttgattata cacttaaatt gaaaatagaa tgattgaata agttgacaga
    35221 ttattttaca ttgacagtaa ttagaacttc ttgagatgta tttaaatcat ttttgtcgct
    35281 tagtataact tttaccactg cttgacctgt tttattacct ttaattatca tatcttcaca
    35341 tgaaagtacg ctcttatcac ttacagatac aataaaattt aaatcttttg gaaggttatc
    35401 agtaacttcc cagtatttat aataactatc aattttcttc gtaattattt ttctcaaatc
    35461 aatgctttca ccttttgcta tatcaacatt atgatattta gagtccagtt tatactgatt
    35521 atcttttgta tcaataaaat ttaaatcgca atttacatcc acaccattat gagaaagaga
    35581 atttactaca tcgtcatcaa tcaaataatt attggcaaga tttattgaat ccatatcttt
    35641 tagacttaaa gcttccttag gaacctcttt tatattatta ttccaaaaaa taaaattgtc
    35701 tacattttca agatatgata taccttttaa atttgaaatg ctctcaccag ttgcataaaa
    35761 ttctccatct aaagccttta tattgcttat agtcatctca tctgggttta tatatctttt
    35821 tacaactgct cttaaatttg catcaggaaa aatatcttta agttgatttt cgctcaaaac
    35881 cttatcattt gaatttattg gacttaaagc tgctgcagaa ataataaatg gtattaaatt
    35941 agctaaattc atagtaccct cccacataaa ttttttatta taataattta atttctatga
    36001 gagggttctg tttccttgtt atatatacct actttcgtac actttattaa tataaattaa
    36061 aataaatttc tactattaaa cttataaaca tattactagt tacgtttttt agcttcacat
    36121 ctaaatattc gcataccttg tgacataaat ttgtcttcat actctgttgt aacattattt
    36181 tcatactcac tatttcctaa atcaagagaa atattcttta atagccaatt atttgcagca
    36241 atttcattta aactaaactc gaataatttt tcattatctg ttttaaagtg aatttcgcca
    36301 tcatcttcca aaaccctatt gtacatttct aaaaatccag aatgagtgag tcttctttta
    36361 ctccatctat ttttaggcca tgggtcacag aaatttatat aaattctcga taattcttta
    36421 gcttcaaagt aatccaaaat attgcttaca tctccccata aaaatagtat attattcaaa
    36481 ttagaagcat ctgctttttc aacagctttt aaaagaactt cttccttaag ttccatggca
    36541 atataattta tgtcagggtt ctgcttagct aaagttgtta taaacttacc ttttccagtc
    36601 ccaaattcaa catgtattgg attatcattt ctaaacttta aattccactt gccttttaat
    36661 ttgtctatat cgtctcttaa aacatatttt gtataactta aaagcttttc atctgcacct
    36721 ttttttcgtc ttcttctcac aataattctc cttacatata aaaaattagt aaaatacatt
    36781 tttattatac attatctcac caaaaaataa aaagaactct cttaaaatag aaatttattt
    36841 ctataaagag agttctaaac ttactaaaaa cttaatctta gtggcaccct gaacaaccag
    36901 agcatcctcc tccaccttga tctttcatat tttcaggaat aactctaaat ccaaatcctg
    36961 tatcttctat tattatatct ccatactgac tatattcatc agagctcatt acaaattgta
    37021 actctccaac ttcataagtt aagtcatctt cttttttctc atctaaagct aatccaaaag
    37081 aaggacctcc acaaccaaca cctgcaaagt atactcttat attattaggt ttatcttgat
    37141 tgtcttttaa gatacttttt aaagtatcaa tagctgtttc tggtaaagta attttcatca
    37201 aatcattccc tttcaaatat tttattaaaa caatatctat tatataccca tacaaggtac
    37261 ataatcaatt agtttttgca tattttacta aaacttctac ctaatattac ctaaggcttt
    37321 aaaatatttt atataatatt t
//
LOCUS       DBGJFF010000028        36506 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.028, whole genome shotgun sequence.
ACCESSION   DBGJFF010000028 DBGJFF010000000
VERSION     DBGJFF010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 36506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36506
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.028"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(47..451)
                     /locus_tag="ACOIAF_001438"
     CDS             complement(47..451)
                     /locus_tag="ACOIAF_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HGZ2668454.1"
                     /translation="MKKYKVIFIVLIIGIISVTGFVVYKNCIKPWKDAETYIDKYMVK
                     QDISKDNIKSITKEKAKKASYEGILYKVYYKDDPRYEYQYFYSDNYYALYVNKVMLQI
                     YDMENNNKLLRSNEELKSVKYPPIYLKYKPDK"
     gene            complement(643..1830)
                     /locus_tag="ACOIAF_001439"
     CDS             complement(643..1830)
                     /locus_tag="ACOIAF_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide family glycosyltransferase"
                     /protein_id="HGZ2668455.1"
                     /translation="MSKIVFFSIPAYGHTNPTIEVVRELVDRGNEVLYYSFNEFKDKI
                     EGAGAKFICCDKYLPELLPGDEKKIGKDFPGLIEMIVDTTISLDEKVCRELKDFNPDC
                     IVSDSLSFWGKLFAQKLNIPYVCSTTTFAFNKHTAKLMKQNFIEIIRMIFGVRRINKK
                     IKLLQRKGYEVKNFISIVSNDNDTDTIVYTSKEFQPMVETFSSKYSFVGPSVSKLIIE
                     PKERKRKLIYISLGTINNKNVSFYKNCINAFKDSDVDVIMSVGRSTDIKSLGNIPNNF
                     EVKNSVEQIAILQKTDVFVTHSGMNSVNESLYYGVPMVLFPQHSEQRMVAKRVVHLGA
                     GIMLKEDKSESIKKAVFQVINDNEYKENATKLSKSFYNAGGSKKAADVILQIICNSRQ
                     VDI"
     gene            complement(1862..2077)
                     /locus_tag="ACOIAF_001440"
     CDS             complement(1862..2077)
                     /locus_tag="ACOIAF_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006774905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HGZ2668456.1"
                     /translation="MVPPKGKFFFGAVVVGERGQIVIPKEAREVFNINAGDKLLVLGD
                     EERGLGIVHQRDLINFIGHVGVVRKEE"
     gene            complement(2105..2296)
                     /locus_tag="ACOIAF_001441"
                     /pseudo
     CDS             complement(2105..2296)
                     /locus_tag="ACOIAF_001441"
                     /inference="COORDINATES: protein motif:HMM:NF024835.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(2415..2543)
                     /locus_tag="ACOIAF_001442"
     CDS             complement(2415..2543)
                     /locus_tag="ACOIAF_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668457.1"
                     /translation="MKDYKYKGYFKGVEIFDNVNDLNLNTLTTVSRFVLVFLFMIT"
     gene            2765..3514
                     /locus_tag="ACOIAF_001443"
     CDS             2765..3514
                     /locus_tag="ACOIAF_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440038.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HGZ2668458.1"
                     /translation="MILYFSGTGNSRYVARKIAQELNDELISLNQLIKEEKTDELIST
                     DKPFIFVCPTYAWRLPIVVTDFIKETKFLGSNRVYFVMTCGGDTAKSINYIQKLCKYK
                     EWQLKGMAEIKMPENYIALFSTTDKETSKQMIKEADKQIYRIISDIRNENEFETITPS
                     GLGGTIKSGIINTVFYKTIISAKGFHYTDKCIGCGKCVELCPLNNINLKNKKPVWKNN
                     CTHCMACICGCPTEAIEYKNKTQNRERYYLE"
     gene            complement(3680..4045)
                     /locus_tag="ACOIAF_001444"
     CDS             complement(3680..4045)
                     /locus_tag="ACOIAF_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HGZ2668459.1"
                     /translation="MEKECQQRMQEITSKFKECRKAISAMGDETRQLILIALLESDFN
                     GIRVGEITKKTHLSRPAVSHHLKILKEAEIVNLRREGTKNYYYLDSKKSQWKSLTELI
                     NLIFVGIQHISENDIRKGD"
     gene            complement(4061..4843)
                     /locus_tag="ACOIAF_001445"
     CDS             complement(4061..4843)
                     /locus_tag="ACOIAF_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HGZ2668460.1"
                     /translation="MNMKYQHIIEVDKELCIGCGLCKNDCPVNNIIIENKKSVIKKQD
                     CLMCGHCAAICPTKAITLTGFDEPPIELTNKPKLDSDELLMAIKSRRSIRKFKDKEVS
                     SEIIKQIIEAGRYTPSAKNSQDVSYIVLDNKKSIYENEAVKFFRKIKPIANIAIKYSK
                     EVEIDDNFFFKHAPIAIMIITKDKISGSLAASNMALMAESYGLGVLFSGYFSDVANNS
                     PKLKKLLGLKRSNHVLTTLVIGYPDVKYRRTAQKEVATVRYL"
     gene            complement(5248..6675)
                     /locus_tag="ACOIAF_001446"
     CDS             complement(5248..6675)
                     /locus_tag="ACOIAF_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HGZ2668461.1"
                     /translation="MSFKNDFLWGGATAANQCEGGYNEDERGLANVDVCPTGKDRTAV
                     ITGKLKMFDFDDEHYYPAKTGIDMYHNYKEDIKLFAEMGFKVYRMSIAWSRIFPKGDE
                     ETPNEKGLQFYENIFKECKKYKIEPLVTITHFDCPMHLVKKYGAWRNRKMIGFYEKLC
                     NVIFRRYKGLVKYWLTFNEINMILHAPFMGAGICFEEGEDVEKIKYQAAHHELVASAI
                     ATKIAHEVDSNNLIGCMFAAGSVYPYSCNPNDVWEATKLDRENYFFVDVQSKGKYPNY
                     ALKYMEQKGITPEMEPGDIELLNKYKVDFISFSYYNSRCVRTDENADDMAEGNIFTSA
                     KNPYLSYSQWGWPIDPLGLRITLNHVYDRYEKPLFIVENGLGAKDIADENGYVEDDYR
                     IDYLREHIKAMHDAVSIDGVDLLGYTTWAPIDLVSAGTGEMEKRYGFIYVDRDNSGNG
                     SLRRMKKKSFEWYKKVIASNGEDLY"
     gene            complement(6736..8664)
                     /locus_tag="ACOIAF_001447"
     CDS             complement(6736..8664)
                     /locus_tag="ACOIAF_001447"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HGZ2668462.1"
                     /translation="MGKYKQLAQEIVNNVGGKENISGLVHCITRLRFTLKDESIANDN
                     VLKNMEGIVTVMKSGGQYQVVIGNHVEAVYKDVVEIIGLDDSSTSSETKKSGNILDKG
                     IDIISGIFQPVLGIMAACGMLKGFNALFVAMGLYSDVSGGYMVINAAGDALFTFLPLF
                     LGYTSAKKFGLKPMLGLALGAAVCYPAIQGSSISAGADVMYTLFKGTMFASPVYIDFF
                     GLPIVSIDYTGTVVPVIFIVYFASRCEKLFNKCVPDLVKFFFVPMLTLLITVPVALIV
                     IGPIATFGSTLISQIVISIRDFSPLLAGAIVGFTWQILVIFGMHWGFIPVYINNVMTL
                     GYDNVMMPFFACTFASSAVVLAIFFKTKDKKLKEMAIPNFISGIFGVTEPAIYGILLP
                     LKKPFIISCIASGIGGAFYGFFNLRKFITGGMGIFELPAMIEPDGGMGNLIVALSGIA
                     ISMVVAFVLTMILYKDEKVEKSKKVRNKGKEEIKEVKSTKLEREIVTSPIKGEVLKLS
                     DIEDAAFASGVLGQGVAIIPSDGKVVAPVDGVVTTLFPSLHAIGILSDTGVEVLIHIG
                     LNTIQLEGRGFEACIKQGDRITKGQTILNFDVDAIKELGYSTVTPIVITNSSQFLDVV
                     ETESKNIELEDNLITVLF"
     gene            complement(8809..9651)
                     /gene="licT"
                     /locus_tag="ACOIAF_001448"
     CDS             complement(8809..9651)
                     /gene="licT"
                     /locus_tag="ACOIAF_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HGZ2668463.1"
                     /translation="MIINKILNNNVVITLDDNDEEVIVMGKGIGYQKSKGNLIDKTKV
                     NKVFRISNKEISNKLQELLNNIPIEHMKLSSEIIEYAQIKLNKKLNESIYISLSDHTY
                     SAIQRMKEGINVKNAILWEIKRFYKEEFEIGMKALDIIENKTGIKLPEDEAGFIAFHI
                     VNAQLSEGHTLASDITKLIQEVLSIVRYHFRIEFHEESVFYYRFIMHLKFFAQRLLLD
                     SAHEGETDKELLSIIKSKYNKEFECVVKIKNFIKKQYNYILTDDELIYLTIHLAKVVK
                     DSNM"
     gene            complement(9966..11366)
                     /locus_tag="ACOIAF_001449"
     CDS             complement(9966..11366)
                     /locus_tag="ACOIAF_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HGZ2668464.1"
                     /translation="MKNKYEDVAQLIIDGLGGKDNIIDLTHCMTRLRFILKDEKKVNA
                     DKLKSIDKVVGVNSTSTQYQVIIGNEVGVVFNAIINKGINTAGDIKSDIKNKNKKNSI
                     LNNIIDTITGCMTPMIPALTASGMIKVILSLCTTFNWLSSNSSTYRVLDIIGDVAFYF
                     MPIILAINAAKKFNVNTSIAVIVVGVFLHPNFSAWVSSGDPISFIGVPIQGVIYAASV
                     IPALLTVWMMSYIEKFIDKLTPSMLKTILNPTLVLLISAPIALIVIGPIGNFLGEGLA
                     SIINLLQGRLGFIMVCLLAAAMPFIVMTGMHHALTPIFISAFATTGQESLILISQVCA
                     NLAQGGATLAVALRSKDSKMKQLASAAWISAIMGITEPALYGVTLKLKKPAMLASIGA
                     GIAGLFAGITHVTLYVPQNSIMAVLGLSGEKGISNIVNGLIVIFIALVAPFIMTYIFG
                     VEKNVKEGIDNVVEEV"
     gene            complement(11405..12076)
                     /gene="pgmB"
                     /locus_tag="ACOIAF_001450"
     CDS             complement(11405..12076)
                     /gene="pgmB"
                     /locus_tag="ACOIAF_001450"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HGZ2668465.1"
                     /translation="MKAVLFDLDGVITDTAKYHFEAWHEIAKSLDIEIDETFNEKLKG
                     ISREESLDKILLSANKVDSYTKEEKLKVCEEKNNIYIKLINSLTYKDILPGINELIKE
                     LKKNNVKLGIASASKNAPRILESLGIKDEFDCIVNPSLLKRGKPYPDIFIEGSKMLNL
                     KQYDCVGIEDSYSGIKAINAANMTSIGVGKKEVLGKCDHIVEDTSLLNYNTLLQAWSK
                     KINLA"
     gene            complement(12078..14468)
                     /locus_tag="ACOIAF_001451"
     CDS             complement(12078..14468)
                     /locus_tag="ACOIAF_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HGZ2668466.1"
                     /translation="MSLIFTTKDQLIEQGFSKDTIKSNETLFTLANGHLGVRGNIEES
                     DFNNEYVDNMGTYVNGFYEESPITYGESAYGYAKQNQTICKLPNAHIVNFSIEGDYFD
                     LSKGITSEHTRILDLNEGILKRSFIWENSCGRKVKVSIERLVSQDIEELMMISYTITP
                     LNFNGKIKFKNKMENSMEKFNCEKVDDPRVASINKKQFKIDVKKYNNKYFMNLVTERS
                     RLGLWCGFDSYITCGNITNETYNVSDECIEREIEVYAKKDERLNLEIVVGYSRVYSDL
                     NLNEERLKSLECLESLKCLESEAHLKSLELVLREACNIGFDKVKNIQRKKMNEFWTTS
                     DVEIEGDKTLQLGIKTNLFHIYQSAGRDGKTNISAKGLTGDGYEGHYFWDTEMYILPF
                     FIYTQPSIARALLDYRYSILDASRERARELGINKGCLYSWRTINGNECSAYYPAGTAQ
                     FHINADIAYGIRTYFEATNDEEFMMQNGLEILIETARFWVEFGDYIESKNNKFCINGV
                     TGPDEYTAIVNNNYYTNLMAKYNLIYAVQMVDKFKHNLFSNEVFRKVKLQEYEVKNWI
                     NAAQNMYLPYDEEKKISKQDDSFLEKSVWDFKNTPKEKYPLLLHYHPLTIYRYQVNKQ
                     ADTVLSALLFPDEFSLEQKKRDFEYYEKITTHDSSLSRSIFGMMASEIGNHEKAYNYF
                     MDTALMDINNLQKNTSDGIHAANMGGTWMSLVYGFAGMKIKNDKLSFEPRIPKHWKNI
                     KFNILFRDNLVSVNIEEYKSYYKLIKGRNITILHDNKEIKLSKNIIEVNNCFKGSN"
     gene            complement(14494..15942)
                     /locus_tag="ACOIAF_001452"
     CDS             complement(14494..15942)
                     /locus_tag="ACOIAF_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HGZ2668467.1"
                     /translation="MKLGKDFLWGGAISANQSEGFYLDGGKGLSQFDKLPLNDKRLKD
                     VCLDEDNILNENNQYYPSHIGIKFCENYKEDIAMLAEIGFKCFRFSISWSRIFPTGEE
                     SEPNEKGLQLYDNIIKELKKYNIEPLITISHFDIPYSLVEKYSGWADRRVVNLYVKYA
                     EILMKRYKDDVKYWIPFNEMNMIMHIPYIGAGLTFIADENQLQKKYQAAHHQLLANAK
                     TIEIGRYINKEFKFGCMLAAGKTYPYTCKPEDVFATLEHERHNLFFSDVQVRGVYPSF
                     MSYYLKSNQIKLIIKEEDLSTLKDNTVDFVSFSYYSSACSSARAEGLEKSKANGPETI
                     KNPYLPKSNSVWQNDPVGLRITMNQLYERYQLPLFIVENGLGTQDIVNEDGKIIDDYR
                     IDYMKEHIKNMILGVNEDGIELIGYLMWGCIDVTSVSEGKMTKRYGIIHVDSDDYGRG
                     SFKRTKKQSAFWYKKVIETDGEILFNDMETHA"
     gene            complement(16347..17234)
                     /locus_tag="ACOIAF_001453"
     CDS             complement(16347..17234)
                     /locus_tag="ACOIAF_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HGZ2668468.1"
                     /translation="MAIYLEMPKLIHEFPFRSLSNEGEVLTTPHWHKEIEILLITEGV
                     VNLFINDKPMQLKKGEIVIIKGGDIHYILPSPGSERLVFQFDISFFNDLILLNEEKES
                     LINLFSSIKKCSRDWSAKIRKSVFDILIDIYNEDLYKIEGYSYAIKGKMYNLIPILYR
                     QIPREIEENTVEYEINSKEILERLNNIFKYVEKNYKQPIKLEDVSYEVGFSVYYFTKF
                     FKKNTGKTFITFLNEYRLEKAKWILLNNNISIVDLVEEVGFGSTKTFYRVFKEKIGVS
                     PLEFKKRYNSLEKKGSVSK"
     gene            complement(17334..17783)
                     /locus_tag="ACOIAF_001454"
     CDS             complement(17334..17783)
                     /locus_tag="ACOIAF_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGZ2668469.1"
                     /translation="MEIREILTEESIILDLKSTTKEGIIQELVDKLYDTGSITLKSKF
                     IKALKHREKIGSTSIGYQMAMPHGKTKYVKKASVVIGKSKKGVDWPSIDGLPVKVIIM
                     FAMPQSGGNEDMILISKIAYILGGKNIGERLLNAHTKKEILDIFYEV"
     gene            complement(17773..19680)
                     /locus_tag="ACOIAF_001455"
     CDS             complement(17773..19680)
                     /locus_tag="ACOIAF_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HGZ2668470.1"
                     /translation="MLVSRQIEFLKLILNENKYRPIKYFKDKLNVSDKTLQKDLKMIE
                     KYLETFNIKIDIKRGYGILIEDLAKKNIDLINSLNIQSKENKSLSVEQRRMEILKYLL
                     LNSNNITSINQLADKYYVSKTSIVNDFKYIEAWIKEYNLKLNKTLEGTKIIGKEVDIR
                     KSIAKMMDDLLDENYEQHDISELTRLDSATFSALINLFDIDSIIFVETIITELEYNLG
                     YTINQPYYINLITHILICLKRIEEGNQIESQEEREISVDNLDELVYKNVTTLIDKIEN
                     RYRVKITKDELMYIYIFLVSSGFSSENNSSYNDESDRLSESEKVSSIMIENMSRFLNI
                     NLKNDDLLQKSLASHVRPMLNRLRYDIQIKNPLLGEIQERFSDVLGLCMMTINIMTEY
                     DNLKNISIDEVSYLATYFQAAIERNMSSKRVIVVCHTGYGTSQLLAARLKREFPEWTI
                     VDIVPMHQLSKRNLDDVDFVISTVNLDLKDKLHIVVSVLLMENDINNIKNALLSKPKK
                     SMNLDTNLLGMYLEDNIYFNFDVAQMESLIGVNLGSKYESISLGKDLNIYIHKQSKLN
                     KGIMNINNVTRTIDIYLEISNQSFLKNILMEISNLYRQKNYINYIIDCKSKEDIKILF
                     KNNLLEKTNGN"
     gene            complement(19949..21844)
                     /locus_tag="ACOIAF_001456"
     CDS             complement(19949..21844)
                     /locus_tag="ACOIAF_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HGZ2668471.1"
                     /translation="MIGKILRSDCIVLDKQLQTKKDVINYMANHLYHGNYINNKEKFI
                     ADINKREKESSTNMGNLIAIPHAKSNVVNQPVMMYLRTDREITDNDGQKLRSFFMIAV
                     PESGSDEHLKIISKLATYLMEENFLKHLLSAETPYEILSIFNNKAAESGDFSNNQNKG
                     LIVGITGCPTGIAHTFMAKKALEEAGQELGYEVKIETHGAIGVENKLTKEDIKRAEYV
                     IIACDKEVQLSRFIGKKVLQKKVVDAKNVSLAKKLIINAENGMAKEIKPGGKTLEDSK
                     EMLQGSEKKGVYKHLMSGVSEMIPFVVIGGIGIAIAFMFGIFASDPTHETYNPIAGFF
                     GQLGGDSAFKLFIPILAGFIAKSIAGRQGFAPAMIAGFMAVIGGSGFIGGMVAGFATG
                     YIALGVMKITENLPDVLKGVNAVLICPLLIAFTSGAIMFFVVNTPASWLNEALQSWLQ
                     SMSGTNKVLLGAILAGMMASDMGGPINKTASAFGLAMFSSNIFEPSAALMVGGMVPPL
                     AISLAMLIFKNKFTKEERKSSLPTAIMGASFITEGAIPFAAADPLRMIPANIIGSCIG
                     GAICMSLDITLMAPHGGIFVIPFACSAPVAYIACILIGTVITALIIGVTKPTIRSENS
                     EVELLGA"
     gene            complement(21880..22758)
                     /locus_tag="ACOIAF_001457"
     CDS             complement(21880..22758)
                     /locus_tag="ACOIAF_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HGZ2668472.1"
                     /translation="MLINMKEMLKVAQENQFAVPAFNIGSGQILKAVVQSANEKNAPV
                     ILAIHPNELSFLGDSFVASCIEEANKSKVPMVIHLDHGENKEQILRAIRCGFTSVMID
                     GSHLPYEENVAISREIVEIAKGLNVSVEGELGTIGTTGTSSEGGTDEIIYTDSKLAKD
                     FIEKTGVDTLAIAIGTAHGIYPKGFKPELKLDLLKEIREVVDIPLVLHGGSSNPDEEI
                     AQAVKLGVCKINISSDVKSAYYKKCRELLEQNPSLYEPDTIYPPCIKAAREVIELKMN
                     LFNAIDKLKCFYKNKN"
     gene            complement(23463..24896)
                     /locus_tag="ACOIAF_001458"
     CDS             complement(23463..24896)
                     /locus_tag="ACOIAF_001458"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HGZ2668473.1"
                     /translation="MKSNQEFLWGGAVAAHQVEGGYNKGGKGISIADVMTAGTHTISR
                     KITDGVIEGLNYPNHEAINFYENYKGDIRLFAEMGYKCFRTSIAWTRIFPKGDESTPN
                     EDGLKFYDDLFDELLKYNIEPVITLSHFEMPYHLVKNYGGWRNRKLIDFFVNFCEVVM
                     NRYKDKVKYWMTFNEINNQSITTNPIYAFTNSGIIYEEQEDKEEVMYQAAHYEFVASA
                     KVVKIGHKINPEFKIGCMVAAMPSYPYSCNPEDMIKFIESNREQLMFTDVHVRGHYPK
                     HTLKLWERKNYNLDITEEDKKILKEGIVDFIGCSYYLTTVVTADKTMKTTGNDSAGKA
                     DVVENPYLKTSDWGWNIDPVGLRFYLNQLYDKYELPIFIVENGFGAEDVLKSDNTVDD
                     DYRIEYLASHIREMKNAIEIDGVDVIGYTVWGCIDPVSFTTGEMKKRYGFIYVDKNND
                     GSGTLKRYKKKSFDWYKNVIKFNGEIL"
     gene            complement(24919..26760)
                     /locus_tag="ACOIAF_001459"
     CDS             complement(24919..26760)
                     /locus_tag="ACOIAF_001459"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HGZ2668474.1"
                     /translation="MKFERESEEIIKYVGGKENIISLVHCATRLRFKLKDTSKTNKEE
                     LLKMKDVLSVVNSGGQYQVVIGNKVTDYFETIIKKAVLQGQNTEEQGEKVSIVSKIFE
                     SISGAFSPLIPALAGAGMIKALLTILTTMKWMSDTSSTYAILSAAGNAVFYFLPIFLG
                     ITLAIKLGANPYVGGVIGAALLDPNFTALIGAKGGANFFGMPVTAIDYSFGVFPIFVA
                     IFIYYYVDKLLKKIILKDLQLFLVPMISLLVMVPLTVILFGPIATNVGDIISNIFMGL
                     FEFNRVIAGAAIGIAMPFLAVLGLHWGFTPITLQNLATMGGDPIEGAAVAAVFAQIGI
                     AIGLFMKVRKSKKNSELKVLAGSTAVTGILAGVTEPIIYGIILRYRRMIPVLAIAGGI
                     GGGICGVFGVTCNAYVFHNIFSMPVYTPFLGYILGIGTALVLGAVLSYFFGLSKEECE
                     EGNEEVTFDEDDGIEEILSPIEGLQIGLSEVEDEVFASGTIGNGMAIIPSKGEVVSPV
                     NGTITTIFPTKHAIGITSDKGVEILIHVGLNTTMLDGKYYTTHMNAGDKVKKGDRIVS
                     FDKDKIEGEGYSIVTPIILTNTNDLADVIVKENKHIKIGTPVIKVVY"
     gene            complement(26886..27719)
                     /locus_tag="ACOIAF_001460"
     CDS             complement(26886..27719)
                     /locus_tag="ACOIAF_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HGZ2668475.1"
                     /translation="MVIKKVFNNNVVFARDNSGQEVILMGKGIGFNKFTKDLVEIDKI
                     EKKFIFNNEESLSELHSLLDRIPLKDVELVSEIIQMGREELGDNLNDSILITLSEHIS
                     YMLKRMDEGLVFQSPLQWEIRQIYPNEYNFSKKAVEYLRKKLDKNIPDSEIAFITIHF
                     ANAQLETKDMQETLLLTKIIDNVLDIIKYHYGIEINEYSFDYTRFISHLRCFIKRQLS
                     GENTNVDTSLLDIIKMKYKSDYNCAKKVKNFLEKTYKWKISENELLYLTLHLNRLSNI
                     K"
     gene            complement(28768..28980)
                     /locus_tag="ACOIAF_001461"
     CDS             complement(28768..28980)
                     /locus_tag="ACOIAF_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGZ2668476.1"
                     /translation="MAILMKTKIKEYREKLLMTQNELAKLVGVRRETIVHLENGKYNP
                     SLKLAMDIAKVFDTTVENLFEFIEEE"
     gene            complement(28981..29397)
                     /locus_tag="ACOIAF_001462"
     CDS             complement(28981..29397)
                     /locus_tag="ACOIAF_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668477.1"
                     /translation="MSKSETGVRSKLILELLVITYFFIAVLATKYIFKGPSTASLPGL
                     SWLIVFGCYSIYNKKYNDLVDESSEFILSKVNKIAIKIMIYSIVIISIFFATPYTSNI
                     NISNLDTGIILIAVLFIQSLSKLVLYIYFDKRGICN"
     gene            complement(29465..29878)
                     /locus_tag="ACOIAF_001463"
     CDS             complement(29465..29878)
                     /locus_tag="ACOIAF_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668478.1"
                     /translation="MSKSELGVKTNLVSELLMISYFFIDSLLKKFIFKEGNLCITFII
                     LFAFWACDFMYNKKYNDLVDEASKLMLLKVDHIASKIMLYSIFLIAVFFSANYTRGLD
                     ISNLDIGVILLAILFIQSLSKLILYIHFDRKGICN"
     gene            complement(30712..31341)
                     /locus_tag="ACOIAF_001464"
     CDS             complement(30712..31341)
                     /locus_tag="ACOIAF_001464"
                     /EC_number="3.2.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
                     /protein_id="HGZ2668479.1"
                     /translation="MKNRKVYMYVFDTMSDWEVAYLTAELNTGRYFKKGLKPLKVITV
                     GIDKNPIITMGGLKVLPEITVDEFNMESKDLLILPGGNTWLSMNHEIILEKVKEALIQ
                     DSIVAAICGATLGLAKKGLLDVRNHTSNDLEFLKMVIPNYSGEKYYKTESVVSDENLV
                     TASGIAPLEFTFQVLKLLDVFESEALNSWLNLYKTHDSKYFFELMNCNK"
     gene            complement(31420..32382)
                     /locus_tag="ACOIAF_001465"
     CDS             complement(31420..32382)
                     /locus_tag="ACOIAF_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGZ2668480.1"
                     /translation="MAKIDNMMSILWMLNSDKKITAKQIAEKLEINIRTVYRYIDVLS
                     ASGVPIISDTGHNGGYSLLNNFTKAPLFFDIEEQKSLLHAALFAKKEGYFLDEALDKS
                     ILKLKMYSNQNQEDILTNHLRALEVIKPIGKVSIESVLKKLENSILKELSVEINYHAG
                     RKAKSKYRIINPYGIVHWNNNWYVVAFCNMRNEVRSFRVDRISEIIETSNTFNRLTEF
                     SASEFFIKGIIPEIKDEQNIIQLVVEGSIDSLDSLSQHWFLGHYLKERTPNKIIFMLE
                     EEVICKYIPNILLPYRKTIQVIEPLSLRKKIIEDLLELIDYYQI"
     gene            complement(33144..>36506)
                     /gene="cbpA"
                     /locus_tag="ACOIAF_001466"
     CDS             complement(33144..>36506)
                     /gene="cbpA"
                     /locus_tag="ACOIAF_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding adhesin CbpA"
                     /protein_id="HGZ2668481.1"
                     /translation="NAGNAEGDANAGNAEEDANAGNAEEDSNAGNAEEDMNALVVEEA
                     TAEDPTSVGDFKISGGEINVDFKFASGVLTILSGKQMTIQNNNPNTPTTHRIVIPKDV
                     KAELIFDGVNIKTASNSPFTLTPDKDGGAYAHVILQDGSENKLESGDVYYPGMRAGKG
                     TTLTIDDSVVNRDIEGEFIVPEGGKIPRDVTLENGDKLKKGDRLTLLDSNNRGSLKVV
                     GNYYSAGIGGGNTESGGNIIINGGAITAIGQESSRNTGYGAGIGGGGNGSGGDITING
                     GTITAKGAYHGAGIGGGYYTTTKNALSPSVVDPSTGSGYSGNITINGGLSYSYGGAHG
                     SAFGDGCVNPGTNRGYRILVTGGTILPYMTGVGDWIFDLSATTGDVIVTGGSLKATTF
                     KSLGGAVAYGDLEKKTKVFMNKISLKYLGQDKVSTTLVDDFKMLIDGKIYPYGAPSYT
                     DDQGVLYLWFSDQFKGSEVSVNLVVIDKITGGNIKPDDFYVKDIGSGSQFLKQYEEFT
                     IDDTPPYLIIKRYDGLPFEGDVEKLFLDWILKDGGIPIITPKGEKLTDKKFMTISSQR
                     LNEETMEPDTNLGSDDLTKQVDAGKYQLSITSTQFSNVYPFNEAYWGHRAYYKAEIIP
                     ADTETSLVVEESKAKTDVFKPTDTFTLNVTISPDKKEGPDCASPRGYVQFYINGKEYK
                     DPVELKTHNKGSESHNYSTASIEWRPLDSNHHIFTTEQTITVKYIGDDINYIEGSEDE
                     KKLKLDMTNVDVDGDGIPDINIDTDGDGRPDINIDTSGDWKPDINIDTDNTGEWKPST
                     EGGNGDGIWKPDKNIDTNGDGNPDTDYNRPAIDTDNDGVDDYWKPDKNVDTGSGGYDT
                     GNPNLNKPDPDNGGNSKPDPDNGGNSKPDSGNGGNSKPDSGNGGNSKPDSGNGGNSKP
                     DSGNGGNSKPDPDNGGNSKPDPDNGGNGKPDPDNGGNSKPDPDNGGNSKPDPDNGGNS
                     KPDIDTDGDGKPNINIDTDGDGKPDINIDTDGDGKPDINIDIDGDGKPDINIDIDGDG
                     KPDINIDIDGDGKPDINIDTDGDGKPDINIDTDGNGKPDINIDTDESLDSNVGQNVQT
                     GDQSNIMLDLALMFISLFFLIKNLTNKYLRRK"
BASE COUNT        11736 a         6525 c         3782 g        14463 t
ORIGIN      
        1 tatttttaaa taaattttaa taaaaatcat tctattttgt cataaatcat ttatctggct
       61 tatatttaag atatattggt ggatacttta cactttttaa ttcctcatta cttcttaaaa
      121 gtttattatt attttccata tcataaattt gtaacattac tttatttaca tatagagcat
      181 aataattatc actataaaaa tattgatact catatcgagg gtcatctttg taatacactt
      241 tgtatagtat tccttcgtaa cttgccttct tggctttttc ttttgtaata ctttttatat
      301 tatcttttga aatatcttgt tttaccatat acttatcaat atatgtttca gcatctttcc
      361 atggtttaat gcagttttta tatacaacaa accctgttac acttataata cctataatta
      421 atactataaa aataacttta tattttttca tattaatcct ccttcattta ttatttaaaa
      481 ctcgatataa tgcactaaaa aaagaatgtt atctttttta ttaaaatata acatccctca
      541 tatttagcat caatatatac gccccaactg gtaggttaaa agaccccact gtcagtactt
      601 tgattaaatt acatgtatgt atatatattt ttaacaaatc tattatatgt caacttgtct
      661 actattacat ataatctgta atattacatc tgctgctttt tttgagcctc ctgcattata
      721 aaagcttttt gatagtttag ttgcattttc tttatattca ttgtcattta taacttgaaa
      781 tactgctttt ttaatacttt ctgatttatc ctcttttaac ataattcctg ctcccaaatg
      841 aacaactctc ttagctacca ttctctgctc cgaatgttga ggaaataata ccataggtac
      901 tccataatat aaactttcat taacactatt catcccagaa tgagtaacaa acacatcagt
      961 tttttgaagt atagcaatct gttcaacact atttttaacc tcaaaattat ttggtatatt
     1021 tcctaaactt ttaatatcag tacttctacc aacagacata atcacatcaa catcagaatc
     1081 tttaaatgca tttatacagt ttttataaaa gctaacattt ttattattaa ttgtaccaag
     1141 cgaaatatat attagctttc tttttctctc tttaggttca ataatcaact ttgatactga
     1201 tggacctaca aatgaatact tactagagaa ggtttctacc attggttgaa attcttttga
     1261 tgtatataca atggtatccg tatcattatc attagaaact atagaaataa aattttttac
     1321 ttcataccct tttctttgta aaagctttat ttttttatta atgcgtctta caccaaaaat
     1381 catacgtata atttctataa agttttgttt catcaacttt gctgtatgtt tattaaatgc
     1441 aaaagttgtt gtagagcata catatggaat attaagtttt tgtgcaaata actttcccca
     1501 aaaacttaat gaatctgata caatgcaatc tggattaaaa tctttaagtt ctctacaaac
     1561 tttttcatct aaagaaatag tagtatctac tatcatctca attaatccag gaaaatcctt
     1621 accaattttt ttctcatctc cagggagtaa ttctggaaga tatttatcac aacaaataaa
     1681 ttttgctcca gcaccttcaa ttttatcttt aaattcatta aaagaataat acaatacctc
     1741 atttcctcta tccactaatt cacgaactac ttcaatagta ggattagtat gaccatatgc
     1801 aggaatgcta aaaaatacta ttttactcat atttcatacc tccttttata attttttaaa
     1861 atcattcttc tttacgtact actcccacat gacctataaa atttatcaaa tcacgttgat
     1921 gaacaattcc taatcctctt tcctcatctc caagtaccaa taatttatct cctgcattta
     1981 tattaaatac ttctcttgct tcttttggaa tgacaatctg accacgttca ccaactacaa
     2041 ctgctccaaa gaaaaacttt cctttaggag gtactataat acctgttttt tcttctgaat
     2101 gattaactaa gtcatctagt ttgacattgt acaattttgc aagtttaatg caactaccta
     2161 tatcaggaat agattctcca ctttcccatt ttgctattga ttgtctagaa acattaagtt
     2221 tttcagctaa ttcttcttgt gtatactggt tgatttttct tagacttttt aaattctcat
     2281 taatcatatt tttcatacct cctatgttta taatattgga tttattattt aaattctatc
     2341 aactaatctt aacattcata tatatattta tatactttta ttatataaat ttattgttca
     2401 ggtaacatat ttccttaagt tatcataaac aaaaaaacaa gcacaaatct actaactgtt
     2461 gttaaagtat ttaagttaag gtcatttaca ttatcaaata tttctacacc tttaaaataa
     2521 cctttatatt tataatcttt cataagcaaa ttattatccc ttctcatata caagtccagt
     2581 tgatgtcttg ttgctaaatt taatggtaat caaattcttt accaataagt ataaaaatta
     2641 ctaatgtgtt ataattttct taactactaa attttacata catttgaata gaatatatca
     2701 ataaattact ataataaata aaaaattaag agttaaaata aaaaatagtt agggagatat
     2761 tattatgatt ttatatttta gcggaactgg taatagccga tatgtggctc gtaaaattgc
     2821 acaagaatta aatgatgaac tgatatcact aaatcaactt ataaaagagg aaaaaacaga
     2881 tgaattaata tcaacagata aaccattcat atttgtatgt cctacttatg catggagatt
     2941 gccaatagtg gttacggatt ttataaaaga aacaaaattt ctaggaagca acagagttta
     3001 ctttgtaatg acttgtggtg gagatacagc aaaatccata aattatattc aaaaactatg
     3061 taaatacaag gaatggcaat taaaaggtat ggcagaaatt aaaatgccag agaattatat
     3121 agctttattt tctacaacag acaaagaaac atcaaaacaa atgattaaag aagcagataa
     3181 acaaatatat aggattattt ctgatatacg caatgaaaat gaatttgaaa ctattactcc
     3241 tagtggctta ggaggtacaa ttaaaagtgg tataataaat acagtttttt ataagacaat
     3301 tattagtgcc aaaggatttc attatactga taaatgcatt ggctgtggaa aatgtgtaga
     3361 gctatgtcct ttaaataata ttaatttaaa aaacaaaaaa ccagtttgga aaaacaactg
     3421 tactcattgt atggcatgta tttgtggttg ccccactgaa gctattgagt ataaaaataa
     3481 aactcaaaac agagaaagat actatttaga ataatatatt cttaatattt aaaccattat
     3541 aaaatgaact atacccattg ttttatacag ttttacatat tttaactgtc tcctaagatg
     3601 ggtatagttc aaaagatagt gtctcaaagt gaacttttta gttcctaaat caatcttttt
     3661 gtattaaatc aagtataatt taatcacctt ttcttatatc attttcacta atatgttgta
     3721 ttccaacaaa tataagattt attagctctg ttagtgactt ccattgtgat ttttttgaat
     3781 caagataata ataatttttc gttccttctc tacgcaaatt aacaatctct gcttctttta
     3841 atatcttaag atgatgagat acagcaggac gagaaagatg tgtttttttt gtgatttctc
     3901 ccactcgtat accattaaaa tcactctcta agagagcaat taaaatcaat tgtctagtct
     3961 catcacccat agcagagata gctttgcgac attctttaaa tttagacgtt atttcttgca
     4021 ttctttgttg acattctttt tccataaaat ccaccctctc ttataagtat ctaacagttg
     4081 ctacttcttt ctgtgcagta cgtctatatt taacatctgg gtatccaatt actaatgttg
     4141 taagaacatg attactgcgt tttagaccta aaagcttttt taattttggc gaattatttg
     4201 ctacatctga aaaatatcca ctaaataaaa cacctagacc ataagactct gccattaatg
     4261 ccatatttga tgctgcaagt gaaccactaa tcttatcttt tgtaattatc attattgcaa
     4321 taggagcatg cttaaagaaa aagttatcgt caatctcaac ttcttttgaa tatttaatag
     4381 ctatatttgc aataggtttt atctttctaa aaaatttcac tgcttcgttt tcataaattg
     4441 actttttatt gtctaaaaca atatatgata catcttgtga atttttagca ctaggggtat
     4501 acctacctgc ctcaataatt tgctttataa tttctgaaga aacttctttg tctttaaatt
     4561 tacgtatact tcttctagat ttaattgcca ttagtaattc atctgaatct agctttggct
     4621 tatttgtaag ttctatagga ggttcatcaa accctgtcaa ggtaatagct tttgtgggac
     4681 atattgctgc acaatgtcca cacattagac aatcttgttt tttaattact gactttttat
     4741 tttctataat tatattgttt actggacaat catttttaca aagcccacat ccaatgcaaa
     4801 gttctttgtc aacttctatt atatgttggt atttcatatt cattcatcta cctttcgttt
     4861 attagtttaa actattataa cacgtagaca ttttttatat tcattggtaa agaatttgat
     4921 taccattaaa tttttaattt ctctattaaa taaatttttc atagaatttt aaagtatcat
     4981 agtttataca taataaaaaa gacaagctta aaacctaagc ttgccttttt catcttttat
     5041 tacaaagtaa attgcactta cttcccatac ttcttaacaa ttttattttc taaaatttta
     5101 tttgtgaatc atactattgt ataaatacaa tgtaaaataa ttctgtattg attttctata
     5161 gttaaaaaag ttaaaaatta tacttattat taatttttat actcttctta tttaatacaa
     5221 agttcttatt taacatagat ttcttgtcta atataaatct tcaccatttg aagcaattac
     5281 tttcttatac cattcaaaac ttttcttttt cattcttctt agacttccat taccactatt
     5341 gtctctatct acatatataa atccataacg cttttccatt tcacctgtac cagcacttac
     5401 taaatcaata ggtgcccatg tagtataacc taataaatcg accccatcta tagaaactgc
     5461 atcatgcata gcttttatat gttctcttag ataatcaatt ctgtaatcat cttctacata
     5521 accattttca tcagctatat ctttagcacc taatccattt tctacaataa atagtggctt
     5581 ttcatatcta tcatatacat gatttagtgt aattctcaaa cctaatgggt caattggcca
     5641 tccccattga ctgtatgaaa gatatggatt tttagcagat gtaaatatgt ttccttcagc
     5701 catatcatct gcattttcat cggttctaac acaacggcta ttgtaatatg aaaatgaaat
     5761 aaaatctact ttatatttat tcaataattc aatatcacca ggttccatct ctggtgtaat
     5821 acctttttgc tccatatact ttaacgcata gttaggatat ttacctttag attgtacatc
     5881 cacaaagaag taattttctc tatctaactt tgtagcttcc catacatcat taggattaca
     5941 actataagga tatacacttc cagctgcaaa catacatcca attaagttat ttgaatcaac
     6001 ttcatgtgct attttagttg caattgcact agctacaagc tcatgatggg cagcttgata
     6061 ttttatcttt tcaacatctt caccttcttc aaagcaaata ccagcaccca taaatggagc
     6121 atgtaaaatc atatttattt cattgaaagt tagccagtac ttcactaatc ctttatatct
     6181 tctaaatata acattgcata atttctcata gaaacctatc atttttcgat tacgccatgc
     6241 accatacttt ttaactaaat gcattggaca atcaaagtgt gtaatagtga ctaaaggctc
     6301 tattttatat tttttacact ccttaaatat attttcataa aattgaagtc ccttttcatt
     6361 tggtgtttct tcatcacctt ttggaaatat tcgactccat gcaatgctca ttctatatac
     6421 tttaaatccc atttcagcaa ataactttat atcttcttta taattatgat acatatcaat
     6481 accagtttta gctggataat aatgctcatc atcaaaatca aacattttta atttccctgt
     6541 aataactgct gttctatctt ttcctgttgg acatacatct acatttgcca atcctctttc
     6601 gtcttcattg tatccacctt cacattgatt ggccgcagta gctcctcccc ataaaaaatc
     6661 attcttaaaa ctcatagtca aagcctccta aaattataac caaagcacta gattatgcta
     6721 atacctcaat ctttactaaa ataagactgt aattagattg tcttctaatt caatattttt
     6781 actttctgtt tctactacat ctaaaaattg tgatgaattt gtaataacaa ttggagttac
     6841 agtactatac cccaactctt ttattgcatc aacatcaaag tttaatatag tttgaccttt
     6901 tgtaattcta tcaccttgtt ttatacatgc ctcaaaacct cttccctcta attgaatagt
     6961 atttaatcca atatgaataa gcacttcaac acctgtatca gaaagaatcc ctatagcatg
     7021 tagactagga aataaggttg taactacccc atctactgga gcaactactt ttccatcact
     7081 aggtattatt gccacccctt gacctagtac accagatgca aatgctgcat cctctatatc
     7141 acttaatttt aatacttcac cttttatagg acttgttact atttctcttt ctaatttagt
     7201 agacttgact tcttttattt cttctttacc cttatttctt acttttttag acttttctac
     7261 tttctcatct ttatataaaa tcatagttaa tacaaaagct acgaccattg atatagctat
     7321 accagaaaga gctactatta agttacccat tcccccatct ggttcaatca ttgctggaag
     7381 ttcaaatatt cccattcctc cagttataaa ttttctaaga ttaaagaatc catagaaagc
     7441 gcctccaata ccactagcaa tacaacttat tataaatggt ttctttaaag gcaataatat
     7501 tccataaatt gctggctctg taactccaaa tataccagat ataaagtttg gtattgccat
     7561 ctcttttagt tttttatctt tagtcttgaa aaatattgcc aacacaactg ccgaacttgc
     7621 aaatgtacag gcaaagaatg gcatcataac attatcatat cctaaagtca taacattgtt
     7681 tatatataca ggaataaatc cccagtgcat accaaaaata acaagaattt gccaagtaaa
     7741 tcctactata gcacctgcca atagtggact aaaatctctt attgaaatta caatttgtga
     7801 aataagtgta gaaccaaatg ttgctatagg acctataact ataagtgcaa caggcactgt
     7861 tattaataat gtaagcattg gcacaaagaa aaacttaact aaatctggaa cacacttatt
     7921 aaataatttt tcacacctag aagcaaaata cactataaat ataactggta cgactgttcc
     7981 agtataatca attgatacaa ttggtagtcc aaagaaatca atatatactg gtgatgcaaa
     8041 cattgttcct ttaaataaag tgtacatcac atctgctcct gctgatatac tgctaccttg
     8101 tatagcagga taacatactg cagctcctag tgctaatccc aacattggtt ttaatccaaa
     8161 cttcttagct gaagtatatc ctaagaacaa aggaaggaat gtaaataatg catctcctgc
     8221 tgcatttatt accatataac caccagatac atcactatat aatcccattg ctacaaataa
     8281 tgcattaaat cctttcaaca taccacaagc tgccattatt cctaacactg gctgaaaaat
     8341 tcctgatatt atatctatac ctttatctag tatattacct gattttttag tttctgaact
     8401 tgtacttgaa tcatctaaac caattatttc aactacatct ttatagacag cttcaacatg
     8461 atttcctata actacttgat attgtcctcc acttttcatt acagtaacaa taccttccat
     8521 gttttttaat acattgtcat ttgctatact ttcatctttt aaagtaaaac gtaatcttgt
     8581 aatacagtgt actaacccac taatattttc ttttccacca acattgttta ctatctcttg
     8641 tgctaattgt ttgtattttc ccatttttct cctccatatt tcgtttaaat taattttttg
     8701 aaatttgaag gtttggccaa ctcaatgtta ccatccaacc ataagtataa ttttgttagt
     8761 atcactcctt tcaatcattc ttaatatact actgaaaaat tatttcactt acatatttga
     8821 gtctttaact actttagcta aatgtattgt taaatatata agctcatcat ctgttaatat
     8881 gtagttatat tgttttttaa tgaaattttt aattttaact acacattcaa actctttatt
     8941 atatttagat ttaataatgc ttagtaattc tttgtctgtc tcaccttcat gtgcactatc
     9001 taaaagcaat ctttgcgcaa aaaactttaa atgcattata aatctataat aaaacactga
     9061 ttcttcatga aattctatcc taaaatgata cctaactatg ctaagtactt cttgtattaa
     9121 cttcgtaata tcagatgcca aagtatgacc ttcacttaac tgagcattca ctatatgaaa
     9181 ggctataaat ccagcctcat cttctggtaa ttttatacct gttttgtttt caataatatc
     9241 tagtgctttc attccaattt caaactcttc tttataaaat cttttaattt cccaaaggat
     9301 tgcatttttt acatttattc cttctttcat cctttgaatt gcagaatatg tatggtcact
     9361 taaggatatg taaatacttt catttagctt cttgtttaac tttatttgtg cgtattctat
     9421 aatttcactt gataatttca tgtgttctat aggaatatta ttgagtaatt cttgtagttt
     9481 gtttgatatt tctttatttg atattctaaa tactttattt actttagttt tatctattag
     9541 attgcctttt gatttttgat aaccaattcc tttacccata acgataactt cttcatcatt
     9601 atcatctaat gtgattacaa cattattgtt taatatctta ttaattatca tgattatcct
     9661 ccttttaagc ataaaaaaag cttatccata ataatgggca tactccttcc taggatactg
     9721 ctattattaa tgaataagct tagcttgtac ttaaacaatt actacctaat tccaattaat
     9781 atgataaaac atcagacata ttttgtcaac tttttatttc catacttcat caatctttta
     9841 tgttgctttt tgtcagttct aataattatt cttattaata ttaacttatt tctttgcatc
     9901 aatataaata caaaacaagt tatagtcaat aataataaaa attctattaa tgtaaatagt
     9961 taatattata cttcctctac tacattatct ataccttcct taacattttt ttcaactcca
    10021 aaaatatatg tcataataaa tggagcaacc aatgctataa atataactat aagtccattt
    10081 actatatttg aaatcccctt ttcccctgat aatccaagta ctgccattat actattttgt
    10141 ggaacatata aagtcacatg agtaattcct gcaaataacc ctgcgattcc tgctcctata
    10201 gatgctaaca tagcaggttt tttaagtttc aatgtaactc cataaagagc tggttcagtt
    10261 atacccatga tagctgatat ccatgcagct gatgctaact gtttcatctt actatctttg
    10321 cttcttaatg ctacagctaa tgtcgcgcca ccttgcgcta aattagcaca aacttgagat
    10381 attaatatta gcgattcttg tcctgtagtt gcaaatgctg atatgaatat aggagtcaat
    10441 gcatgatgca tccctgtcat aacgataaat ggcattgcag ctgctaataa acaaaccatt
    10501 ataaatccta accttccttg tagtaaattt attatactag ctaatccctc tcctaagaaa
    10561 tttcctattg gtccaattac tattaatgct attggcgcac ttattaataa tactaaagtt
    10621 ggatttaaaa ttgtttttaa catgctaggt gttaatttat caatgaattt ttctatatat
    10681 gacatcatcc atactgttaa aagtgcaggg ataacagatg ctgcatatat aacaccttga
    10741 attggtactc ctataaatga tatagggtcc ccacttgata cccatgcaga gaaatttgga
    10801 tgtaaaaaca cacctactac tataactgct attgacgtat taacattaaa ttttttagct
    10861 gcatttattg caagtattat aggcataaag taaaatgcta catctcctat tatatctaat
    10921 actctatatg tacttgaatt tgatgatagc caattaaaag tggtgcaaag agataatata
    10981 acttttatca tcccagatgc tgtaagggct ggtatcatag gtgtcataca accagttatt
    11041 gtatctatta tattatttaa aatactattt tttttatttt tattttttat atcgctttta
    11101 atatctcctg cggtatttat ccccttattt attattgcat taaatacaac acctacctca
    11161 ttacctatta tgacctgata ttgagttgaa gttgagttta ctccaactac cttatctata
    11221 gattttaatt tatctgcatt gactttcttt tcatctttta agatgaacct taatcgtgtc
    11281 atacaatgag ttaagtcaat tatattatct tttcctccaa gtccatcaat tatcaattga
    11341 gctacatctt catatttatt cttcatacct tatcctctct tttaaaataa tttttaatat
    11401 agttttaagc caaattaatc tttttactcc aagcttgtaa tagtgtattg taatttaata
    11461 agctagtatc ttctacaata tggtcacatt ttcctagtac ttcttttttc cccacaccaa
    11521 ttgatgtcat attggcagca tttatagctt taatacccga ataagaatct tctattccta
    11581 cacaatcata ttgttttaaa tttagcatct tgctcccttc aataaaaata tcaggatatg
    11641 gttttcccct ttttaataaa gatggattta ctatacaatc aaattcatct tttattccca
    11701 aagattctaa tatcctaggt gcattcttac tagcagatgc tattcccaat ttcacattat
    11761 tttttttaag ttcttttata agctcattta tccctggtaa tatatcttta tatgttaatg
    11821 agtttattaa ctttatataa atgttatttt tttcttcaca cacctttaac ttttcctctt
    11881 tagtataact atctacttta ttagcagata acaatatttt atctaatgat tcttccctgc
    11941 ttatgccttt taatttttca ttaaaagttt catctatttc tatatctagc gattttgcta
    12001 tctcatgcca agcttcaaaa tgatacttag cagtatctgt tataactcca tcaagatcaa
    12061 ataagactgc tttcatatta attactccct ttaaaacaat tattaacttc tattatgttc
    12121 tttgataatt ttatttcttt attatcatgt aatatggtta tatttctacc tttgattaac
    12181 ttataataag atttatattc ttctatattt acagaaacta aattatctct aaataatata
    12241 ttaaatttaa tatttttcca gtgtttaggt atccttggtt caaaacttaa tttatcattt
    12301 tttattttca ttcctgcaaa tccatatact aaactcatcc atgttccacc catattagca
    12361 gcatgtattc catcacttgt atttttttgt aggttattaa tatccattaa tgctgtgtcc
    12421 ataaaatagt tataagcttt ttcatgattt ccaatttcac ttgccatcat tccaaatatt
    12481 gaccttgaca aagatgaatc atgagtagtt attttttcat agtattcaaa atctctcttc
    12541 ttttgttcta agctaaactc atcagggaaa agcaacgctg aaagtacagt atctgcttgt
    12601 ttatttactt gatatctata tattgttaat ggatgataat ggagaagtaa tggatatttt
    12661 tcttttggtg tatttttaaa atcccatact gatttttcta aaaatgaatc gtcttgtttg
    12721 cttattttct tctcttcatc atatggtaaa tacatatttt gagcggcatt tatccagttt
    12781 ttcacttcat attcttgtaa tttaactttt ctaaatactt catttgaaaa taaattatgc
    12841 ttaaatttat ctaccatctg tactgcatat attaaattat acttagccat taaattagtg
    12901 tagtaattat tgtttactat tgctgtatat tcatctggtc ctgttactcc atttatacag
    12961 aatttattat tttttgattc tatataatct ccaaattcaa cccaaaatct tgctgtttct
    13021 ataagaattt ctaagccatt ttgcatcata aattcttcat cattagttgc ttcaaaatac
    13081 gttctaatac cataagctat atctgcattt atatgaaact gtgctgttcc tgctggataa
    13141 tatgcactgc attcattacc atttatagtt ctccatgaat ataagcaacc tttatttatt
    13201 ccaagttctc tagctctttc tcttgatgca tctaagattg agtatctata atctaataaa
    13261 gctctagcaa tggatggttg agtatatata aaaaatggta atatatacat ttctgtatcc
    13321 cagaaataat gcccctcata gccatcacct gtaagacctt ttgctgatat attagtcttt
    13381 ccatctcttc ctgctgattg ataaatatga aataaattgg tttttatccc cagttgaaga
    13441 gttttatcgc cttcaatctc tacatcagag gttgtccaaa actcattcat tttctttctc
    13501 tgaatatttt tgactttatc aaatccaata ttacatgctt ctcttaaaac caattctaat
    13561 gattttaaat gtgcttctga ttctaaacat tttaacgact ccagacattc taatgatttt
    13621 aaacgttctt catttagatt taaatcagaa tatactctac tatagcctac aacaatttct
    13681 aaatttaacc tttcatcttt tttggcatag acttctattt ctcgctcaat acattcatct
    13741 gaaacattat aggtctcatt tgtaatattt ccacaagtaa tatatgaatc aaacccacac
    13801 cataacccta atctacttct ttctgtaact aaattcataa aatacttatt gttatatttt
    13861 tttacatcaa ttttaaattg cttcttattt atagatgcca ctcttgggtc atctactttt
    13921 tcacaattaa acttctccat actattttcc attttatttt taaattttat ttttccatta
    13981 aaattcaatg gtgttattgt atatgaaatc atcattaatt cttctatatc ttgtgatact
    14041 aatctttcta tagatacttt aacttttcta ccacaagagt tttcccatat aaatgaacgt
    14101 ttaagaatcc cttcatttaa atctagtatc ctagtatgtt cacttgtaat accttttgat
    14161 aaatcaaaat aatctccttc aatagagaaa tttactatat gagcattagg taacttacag
    14221 atagtctgat tttgttttgc atagccataa gcgctttctc catatgtaat tggagattct
    14281 tcataaaatc catttacata tgtacccata ttatctacat actcattatt aaaatcagac
    14341 tcttcaatat tccctctaac ccctaaatgt ccattagcta gtgtaaacaa cgtttcattg
    14401 gattttattg tatccttact aaacccttgc tctataagtt ggtccttagt agtaaatatt
    14461 aaactcataa taacactcct ttcttaaaat gaattatgca tgagtttcca tatcattaaa
    14521 taaaatttct ccatctgttt ctattacttt tttataccaa aaagcacttt gttttttagt
    14581 tcttttaaat gagcctcttc catagtcatc agaatcaaca tgaattatgc catatcgttt
    14641 tgtcatcttt ccttcactta cactggttac atctatacat ccccacataa gatatcctat
    14701 aagctctatt ccatcttcat ttactccaag aatcatattt ttaatatgtt ctttcatgta
    14761 atctatacga taatcatcaa ttatttttcc atcttcattt acaatatctt gagtacccag
    14821 tccattttct actataaata aaggtaactg atacctttca tataactgat tcatagttat
    14881 tcttaaccct acagggtcgt tttgccatac actatttgat tttggaagat atggattttt
    14941 tatggtttct ggtccgtttg cctttgattt ttctaaacct tcagctctag ctgaactaca
    15001 agcactagaa taatagctaa aagatacaaa atctacagta ttatccttaa gtgttgataa
    15061 atcttcctct tttattatta attttatttg atttgatttt aaataataac tcataaagct
    15121 tggatacaca cccctaactt gaacatcaga aaaaaacaaa ttatgccttt catgctctaa
    15181 agttgcaaat acatcttctg gtttacatgt gtatggatat gtttttccag cagctaacat
    15241 acacccaaac ttaaattctt tatttatata ccttcctatt tctatagttt ttgcattagc
    15301 taataattga tggtgtgctg cttgatattt tttctgaagt tgattttcat cagctataaa
    15361 agttaaccca gcacctatat atggaatatg cattatcata ttcatttcgt taaaaggaat
    15421 ccagtatttg acatcatctt tatatctttt catcaaaatt tcagcatatt ttacatataa
    15481 attaaccact cttctatcag cccacccaga atatttttca actagagaat aaggtatatc
    15541 aaagtggctt atcgttatta atggctctat attatatttt tttaattctt ttattatatt
    15601 atcataaagt tgtaatccct tttcatttgg ttcactttct tctcctgttg ggaatattct
    15661 tgaccaagat atagaaaatc taaaacattt aaaacctatt tctgctaaca ttgctatatc
    15721 ttctttgtaa ttttcacaaa atttaatccc tatatgactt ggatagtatt gattattttc
    15781 atttaaaata ttatcctcat ctaaacatac atcttttaat ctcttatcat ttaatggtag
    15841 tttatcaaac tgagataaac cttttccccc atctaaataa aatccttcac tttgattggc
    15901 tgaaattgcc cctccccata aaaaatcttt ccctaatttc atgcatctac ctctttcaca
    15961 atataaagtt ttataatttc tatctcatat ttagttataa gatttttttc atactatacc
    16021 ttttaaataa ttcaatttaa aaatccaatt atatttttta ctcactataa ttaatattgt
    16081 ctattttcaa tactttcata ttaaatatca atattagata tctatgtata taattatata
    16141 tatagcaaat acatttttat atcctcatta ttgttatctt attctcatat attgctgtat
    16201 atttatatat ataaaaggat ttatttcgaa atagaggttg tattgagaac taaaatttag
    16261 actttataaa tgaatggaat tttcattgtt gaaatactga aaatatattt gatttcatat
    16321 aaaagaaagt gcctcatgaa ctaagttcat tttgagacac tccctttttt ctccaagcta
    16381 ttatatcttt ttttaaattc taatggagag actccaattt tttctttaaa tactctgtaa
    16441 aaagtttttg tacttccaaa accaacttct tcaaccaaat ctacaatgga tatattgtta
    16501 ttaagtaata tccactttgc tttttctaat ctatattcat ttaaaaaagt tataaatgtt
    16561 tttcctgtat tttttttgaa aaatttagta aaataatata cactaaatcc tacttcataa
    16621 gacacatctt ctaattttat aggttgttta taattttttt ctacatattt gaatatattg
    16681 ttcaatctct ctaatatctc tttagagttt atttcatatt ctactgtatt ttcttcaatt
    16741 tctctaggaa tctgtctata taatatagga attaaattat acattttacc ttttattgca
    16801 taactatatc cttctatttt gtataggtct tcattgtaga tatctatcaa tatatcgaaa
    16861 actgactttc ttatttttgc tgaccaatct ctactacatt tttttatact actgaataaa
    16921 ttaattaagc tttctttttc ctcatttaat aaaattaaat cattaaaaaa agatatatca
    16981 aattgaaata ctaatctttc acttcccggt gatggcagta tataatgaat atctcctcct
    17041 tttattatca ctatctcgcc tttttttaat tgcataggct tgtcatttat aaatagattt
    17101 actacacctt ctgttataag taatatttct atttccttat gccagtgtgg tgttgttaat
    17161 acttctcctt catttgatag actacgaaaa ggaaattcat gtattaattt aggcatttct
    17221 aaatatattg ccatatatat cctctcctct ctagtgtcta aaaattaatc tatactattt
    17281 aaaagaatac acattttatt ttaaaaacct caaatttata atttatacaa tacctacact
    17341 tcataaaata tatctaatat ttcttttttt gtatgtgcat ttaaaagtct ttcacctata
    17401 tttttaccac ctaaaatgta agctatttta gatattaata tcatatcttc attgcctcca
    17461 gattgtggca tagcaaacat aattattact tttacaggta agccatctat acttggccaa
    17521 tctacacctt ttttagattt acctattact acagaggcct tctttacata ttttgttttt
    17581 ccatgtggca tggccatttg ataacctata ctagtagagc ctattttttc tctatgtttt
    17641 aatgccttta taaacttact ttttaaagtt atacttcctg tatcgtataa tttatctact
    17701 aattcctgta ttatgccttc ttttgtagtt gatttcaaat ctaaaataat gctttcttct
    17761 gttaatattt ctctaatttc catttgtttt ctccaataga ttattcttaa ataatatttt
    17821 aatatcctct ttgctcttgc aatctattat atagtttata tagttctttt gcctatataa
    17881 atttgatatt tccattaaaa tattctttaa aaaactttga ttactaattt ctaaataaat
    17941 atcaatcgtt ctagtaacat tattgatatt cattatccct ttatttaatt tactttgctt
    18001 atgtatatat atatttaaat cttttcctaa tgaaatagat tcatatttac tccctaaatt
    18061 cacacctatt aaactttcca tctgagctac atcaaaatta aaatatatat tatcttctaa
    18121 atacattcct aataagtttg tatctaagtt catacttttt ttaggtttag aaagtaaagc
    18181 attttttata ttattaatat cattttccat taatagtact gatacaacta tatgtaattt
    18241 atctttcaaa tctaagttta cagtagatat tacaaagtct acatcatcta aatttcgctt
    18301 actcaactga tgcataggaa ctatatcaac tatagtccat tcaggaaatt ctctttttaa
    18361 tctggctgca aggagctgag atgttccata tcctgtatga caaactacta ttactctttt
    18421 actactcata tttctttcta ttgccgcttg aaaatatgtg gcaagatacg agacttcatc
    18481 tatactaata ttcttcaaat tatcatactc tgtcataata tttatagtca tcatacataa
    18541 tcccagcaca tctgaaaatc gctcttgtat ctctcctaac aacgggtttt ttatttggat
    18601 atcatatctt aatctgttta acataggcct tacatgagat gctagacttt tctgtagtaa
    18661 gtcatcattt ttgagattta tatttaaaaa tcgtgacata ttctctatca ttatagatga
    18721 aactttttca ctttcactta atctatctga ttcatcattg taactactat tattttcgct
    18781 actaaatcca gatgatacca aaaaaatata tatgtacata agttcatctt tcgttatttt
    18841 tactctgtat ctattttcta ttttatctat aagtgtagtg acatttttat atactaactc
    18901 gtccaaatta tctacagaaa tttctctttc ttcctgagat tctatttgat taccttcttc
    18961 gattctcttt aaacatataa gtatatgggt aattaaattt atatagtagg gctgatttat
    19021 agtatatcct aaattgtatt ctaattctgt aattatagtt tctacaaata taatagaatc
    19081 aatatcaaat aaatttatca atgcactaaa tgtagcagaa tctaatcttg ttaactcaga
    19141 tatatcatgt tgttcataat tttcatctaa taaatcatcc atcattttgg caatcgattt
    19201 ccttatatcc acttctttac ctataatctt agttccttct aaagttttat taagttttaa
    19261 attgtattct tttatccatg cctctatata tttaaaatca tttactatag aagttttact
    19321 tacataatat ttatctgcta actgatttat tgaagttata ttatttgaat ttagcaataa
    19381 atactttaaa atctccattc ttctctgctc tactgataaa gatttatttt ccttcgattg
    19441 tatatttaaa ctattaatta agtctatgtt cttctttgct aaatcttcta ttaatattcc
    19501 atagcctctt tttatatcaa tttttatatt aaaagtttct aaatatttct caatcatctt
    19561 caaatctttt tgtaaagttt tatcagatac atttaattta tctttaaagt attttatagg
    19621 tctatattta ttttcgttta gtatcaattt aagaaattca atttgcctcg acacaagcat
    19681 acttagccct ccctacctat gtttatatat ttctatgttt atattaagta ctattttaac
    19741 atatttacta actatgtaaa attactaaga tacttctgta tcataaaaga atttatgata
    19801 caccttacta aattgatata ataagataat ataatttcaa aataaaaatt aaggatattt
    19861 agaagtagtt aactgattct aaatatcctt attattattc tatatacttg ttgttattcc
    19921 attaattatt tttactatta tttataattt aagcaccaag taattcaact tcactatttt
    19981 cactccttat agtaggcttt gttaccccta taatcaatgc tgttattact gtaccaatca
    20041 atatacaagc tatatatgca actggtgcac tacatgcaaa tggtattaca aatattcccc
    20101 catgtggtgc cattaaagtt atatctaaac tcatacatat tgccccacct atacaagaac
    20161 caattatatt tgctggaatc attcttaatg ggtctgcagc tgcaaacggt attgcgcctt
    20221 ctgttatgaa agatgccccc attatagcag ttggtagaga tgactttctt tcttctttag
    20281 taaacttatt tttgaatatt aacatagcca atgatatcgc taatggaggt accattcctc
    20341 ctaccattaa tgctgctgat ggttcaaata tattacttga aaacatagcc aatccaaaag
    20401 ctgaagctgt tttattaata ggtcctccca tatctgatgc catcatccct gctaatattg
    20461 cacctaataa tactttatta gttccagaca tactttgtaa ccaagattgt aaagcttcat
    20521 ttaaccatga tgctggcgta ttaactacaa agaacattat agctccactc gtaaatgcaa
    20581 tcaaaagtgg acatatcaat acagcattta ctccttttaa tacatctggt aaattttctg
    20641 ttatcttcat tactcctaaa gctatatatc ctgttgcaaa accagctacc ataccaccta
    20701 taaaccctga acctccaata actgccataa aaccagctat catagcagga gcaaatcctt
    20761 gtcttcctgc tattgattta gctataaaac cagcaagtat cggtataaat aacttaaatg
    20821 ctgaatcgcc tccaagttgt ccaaagaatc cagctatagg gttgtatgtt tcatgagttg
    20881 ggtctgatgc aaagatacca aacataaatg ctatggctat accaattcca cctatcacta
    20941 caaatggaat catttcagat acaccactca ttaagtgctt ataaacaccc tttttctctg
    21001 acccttgaag catttctttt gaatcttcta aagtctttcc acctggttta atttctttag
    21061 ccattccatt ttcagcgttt ataattaatt ttttagctaa tgaaacattc ttagcatcta
    21121 ctactttctt ttgtagcact ttctttccta taaatctact cagttgaact tctttatcac
    21181 aagctattat tacgtattca gctcttttta tatcttcttt agtcaactta ttttctacac
    21241 ctatagctcc atgagtttct attttaactt catatccaag ttcttgccct gcttcctcta
    21301 atgctttttt tgccataaaa gtatgagcta tacctgttgg acatcctgtt atcccaacta
    21361 taagaccttt attttgatta ttactaaaat ctcctgattc tgcagcttta ttattaaata
    21421 tacttaatat ttcgtatgga gtttctgcac ttagtagatg cttaagaaaa ttttcttcca
    21481 ttagatatgt agctagttta gatataatct taagatgttc atctgaacct gattctggaa
    21541 ctgctatcat aaaaaatgac ctaagttttt gaccatcatt atctgtaatc tctctatctg
    21601 ttcttaaata catcattaca ggttgattta ctacatttga cttagcatga ggtatagcta
    21661 ttaaattacc catattagtt gatgattctt tttcacgttt attaatatca gctataaact
    21721 tttctttatt atttatataa tttccatggt acaaatgatt tgccatataa ttaattacat
    21781 ctttttttgt ttgtaattgt ttatctagta caatacaatc gcttcgtagt attttaccta
    21841 tcataaattc tcccctcctt ttaatttgtt acctcataat taatttttat ttttataaaa
    21901 gcactttaac ttatctatag cattgaatag attcattttt aactctataa cttctcttgc
    21961 agcttttata catggtggat atatagtatc tggctcatat aagcttgggt tttgttctaa
    22021 tagttctctg cacttcttat aatatgccga cttaacatca ctcgaaatat ttattttaca
    22081 aactcctagt ttaactgctt gtgctatttc ttcatctgga tttgatgaac caccatgaag
    22141 aactaaaggt atatctacta cttctcttat ttcttttaat aaatctagtt taagctctgg
    22201 tttaaaacct tttggatata taccatgtgc tgtaccaatt gcaatagcta gtgtgtctac
    22261 tcctgttttt tcaataaaat ccttagctaa ttttgaatct gtatatatta tttcatcagt
    22321 accaccttca cttgatgttc ctgttgttcc tattgttcca agttctcctt ctacggacac
    22381 atttaatcct tttgctattt ctactatttc cctagatata gctacattct cttcatatgg
    22441 taaatgtgag ccatcaatca ttactgatgt aaatccacat cttattgccc ttaatatttg
    22501 ttctttattt tccccatgat ctaagtgtat taccataggt actttactct tatttgcttc
    22561 ttctatgcaa ctagctacaa agctatctcc tagaaaactt agctcatttg ggtgtattgc
    22621 aagtattact ggtgcatttt tttcgttagc actctgtact actgctttaa gtatttgccc
    22681 acttcctata ttaaatgctg gtacagcaaa ttgattttct tgtgccactt ttagcatttc
    22741 cttcatattt attaacatat tgtacctccc gatattttaa attttaggtt gaatttgctt
    22801 cttgttaatt ttgttgttgt ttatatttaa ataatatcag ggtaatgaag gtcagaaaat
    22861 aacaatcgtt ttccttagtg aaaaggaact tttattatac actctaagtt ccttttcact
    22921 aaataatttt tagaagtctg tatcatagtt aattatattt ctcatgtaca tcttctcttt
    22981 ttaagtgttt tatgctttag ccttcttaac tctttccaat aaacaattag atagtttagc
    23041 tataaattta aagatattcg tatcgtagtt agtactatat tggaatcaaa aaaattaaaa
    23101 agatactaga tagcagatac caattactaa tacaccataa gtcatagata cttctatctt
    23161 aaattcttgc atattagtta caatagaaat taaataggac tattataaaa aacgtaaggt
    23221 aaggctgaca tttattctcc aactgtacaa atgaatacta atatagctcc ttctaattaa
    23281 atcatactat ataaatcata tatatcccaa tttttaacct tacctcttta tatttacttt
    23341 ttaataaaaa aagatattga ctaaatagtc aatatctttt agtggattta aatacccacg
    23401 attttaatct aatttttaat ggtttcctta acataatctt ataaatatta aaatttatct
    23461 acctataaaa tttctccatt aaattttatt acatttttat accaatcaaa agatttcttt
    23521 ttatatcttt taagagtccc acttccatca ttatttttat ctacatatat aaatccatat
    23581 ctttttttca tctctcctgt tgtaaatgat actggatcta tacatcccca aacagtatac
    23641 cctataacat caacaccatc aatttcaatg gcatttttca tttcccttat atgactagcc
    23701 aaatactcta ttctataatc atcatctaca gtattatctg attttaatac atcttctgct
    23761 ccaaatccat tttcaacaat gaatatagga agttcatact tatcatatag ttgattaagg
    23821 taaaatctta gacctacagg gtctatattc catccccagt cagaagtttt taagtatgga
    23881 ttttcaacta catcagcctt tcctgccgag tcattacctg ttgttttcat agttttatca
    23941 gctgttacta cagtagttaa ataataacta catccaataa aatcaactat tccttctttt
    24001 aagatttttt tatcttcctc tgtaatatct aaattatagt tttttctttc ccataatttt
    24061 agtgtatgtt ttggataatg acctcttaca tgtacatcag taaacataag ttgttctcta
    24121 ttagattcta taaatttaat catatcctct ggattgcaag aataaggata acttggcata
    24181 gctgccacca tacatccaat tttaaactca gggtttattt tatgacctat cttcacaacc
    24241 tttgcacttg ctacaaattc ataatgagct gcttgataca taacttcttc tttgtcttcc
    24301 tgctcttcat aaattattcc cgagttagta aatgcataaa taggatttgt agttatactt
    24361 tgattattta tttcattgaa agtcatccag tacttaactt tatccttgta tctattcatt
    24421 accacttcac agaagtttac aaagaaatct attaattttc tatttctcca tccaccatag
    24481 ttttttacta aatgataagg catttcaaaa tgacttaaag ttattactgg ctctatattg
    24541 tattttaata attcatcaaa taaatcatca taaaatttca atccatcttc atttggagtt
    24601 gattcatcgc cctttggaaa aattctagtc caagcaatac ttgtcctaaa acacttatat
    24661 cccatttcag caaataatct aatatctcct ttataatttt cataaaaatt aatggcttca
    24721 tgattaggat aatttaaacc ctcaattact ccatctgtta tctttcttga aatagtatga
    24781 gtacctgcag tcatcacatc tgcaatactt attccttttc cacctttatt atatccacct
    24841 tcaacttgat gggcagcaac tgcccctccc cataaaaatt cttgattaga tttcatagaa
    24901 atatctcctc cagtataatt aataaacaac ttttattaca ggcgttccaa ttttaatatg
    24961 tttattttct ttaactatta catctgctaa atcatttgtg ttagttagta ttattggtgt
    25021 aacaatacta tacccttcac cttctatttt atccttatcg aaagaaacaa tcctatctcc
    25081 ttttttaact ttatctcctg cattcatatg agtagtataa tactttccat ctaacatagt
    25141 agtgttaaga cctacgtgta ttaatatttc tacaccttta tcagaggtaa ttcctattgc
    25201 atgctttgta ggaaaaatag ttgttattgt tccatttaca ggagacacaa cttctccctt
    25261 tgaaggtatt attgccatgc cattacctat agttcctgat gcaaacactt catcttctac
    25321 ttcagataac cctatttgta agccttctat tggagataat atctcctcta taccatcatc
    25381 ttcatcaaaa gtaacttctt cattaccttc ttcacattct tctttactca atccaaagaa
    25441 atatgacaat actgctccta gcactaatgc tgttcctatt cctaatatat atcctaggaa
    25501 tggtgtataa actggcattg agaatatatt atggaataca tatgcattac aagtaacacc
    25561 aaatacacca catataccac caccaatacc tcctgcaata gccaatactg gaatcattct
    25621 tctgtatctt aatataattc cataaattat aggttctgtt actcctgcta aaattcctgt
    25681 aactgctgtt gaacctgcta atacctttaa ttctgaattt tttttagatt ttctaacctt
    25741 cataaataaa cctattgcaa tacctatttg agcaaatact gctgcaacag ctgcaccttc
    25801 tattgggtct ccacccattg tagccaagtt ttgcaaagta attggtgtga agccccagtg
    25861 tagtcctaat actgctaaga atggcatagc tatacctatt gcagctcctg caattactct
    25921 gttaaattca aataatccca taaaaatatt ggatattata tcaccaacat tagttgctat
    25981 tggtccaaat aaaatcacag ttaaaggaac catgactaat aatgatatca tcggtactaa
    26041 gaataattgt aaatccttta atataatctt ctttaaaagc ttatcaacat agtaataaat
    26101 aaatatagca acaaatatag ggaatacacc aaaagaataa tcaattgctg ttactggcat
    26161 cccaaaaaag ttagcaccac cttttgctcc aattaaagct gtaaaattag gatctaacaa
    26221 agctgctcct atcactccac caacatatgg atttgcacca agttttatag ctaaggtaat
    26281 acctaagaat attggtaaaa agtaaaatac agcatttcca gctgctgata atatagcata
    26341 agtgctactt gtatcagaca tccatttcat agtagttagt attgtaagca gtgccttaat
    26401 cattcctgct ccagctagtg caggtattaa tggagaaaat gcacctgaaa ttgattcaaa
    26461 tattttagaa actattgaaa ctttttctcc ctgttcttca gtattttgtc cttgtaaaac
    26521 tgcttttttt ataatagttt caaagtaatc agttacttta tttccaatta ccacttggta
    26581 ttgtcctcca ctatttacta cagatagtac atccttcatt tttaataact cttctttgtt
    26641 agtttttgaa gtatctttca gcttaaaacg tagcctagtt gcacaatgaa ctaaactaat
    26701 aatattttct tttcctccaa catattttat aatttcttca gactctcttt caaatttcat
    26761 acaatctatc ctccattttc ataattttat gtataaaaat agacctaaat caatacaatt
    26821 agatattaaa aactaattat accaacttag gtcatgcctg ccaaacagta acaatcctac
    26881 actatttact ttatatttga taatctattt aaatgcaatg ttaagtacaa caattcattt
    26941 tcgctaattt tccatttata cgtcttttca agaaagtttt ttactttttt tgcacaattg
    27001 taatcacttt tatatttcat ttttattata tctaacaatg atgtgtctac atttgtattc
    27061 tcaccactta attgcctttt tataaaacat cttaaatgac ttataaacct tgtgtagtca
    27121 aaggaatatt catttatttc gattccatag tgatacttta tgatatctaa tacattatca
    27181 attatcttag ttaacaataa agtttcttgc atatcttttg tttctaactg agcatttgca
    27241 aagtgtatcg taataaatgc tatttcagaa tcaggaatat ttttatctaa tttttttctt
    27301 aaatattcta cagctttctt actaaaatta tattcattag gatatatttg tcttatttcc
    27361 cattgaagtg ggctttgaaa tactaatcct tcatccattc tttttaacat ataacttata
    27421 tgttctgata atgtgattaa tatactatca ttaagattat cacccaattc ttctcttccc
    27481 atttgaataa tttcacttac aagctcaaca tcttttaaag gtatcctatc tagtagtgaa
    27541 tgtaattcac ttaatgattc ctcattattg aatataaatt tcttttcaat tttatctatt
    27601 tcaactaaat ctttagtaaa tttattaaat ccaattcctt ttcccattaa tattacttct
    27661 tgtcctgaat tatctctggc aaatacaaca ttgttgttaa atactttctt tataaccata
    27721 aactacctcc actgtaaaca aaaagagacc taaaaaagac taataatacc ttaagcattt
    27781 actagaattt ttaggtcacg cctgattaca tctatctcag taacgatccg tttatttatc
    27841 tgtatgataa ctttattttc attataaatt aataactgta cttagtcaat atataatttc
    27901 atgtatatct ctactagatt attgtcataa gtattcactc attctacttt ttttatatga
    27961 ttcatataat ctaaacatgc taaaataagt tttgaagtta tttcataaca aaattaaggt
    28021 catatatttt ttattatata tactcgatta tttatccata tataaggaaa tcctttattt
    28081 tgtaactaca ataactgcct aagttttgat tttcttttat cttttacttc tccttattaa
    28141 aaaataattc ttttactgtt gtatctaagg ctctaaacag tttaattatc aaatcattgt
    28201 ttggattatt aaccttacct aacttactat atagcttttt aactgtatat ctttaataga
    28261 ccttttggta tgctaatcca ttttataaca ctaattcttg aatcaagcat atattaccct
    28321 tacaaagcat atcttatacc ctctgtagag ttatgatagt tgatgaacct gaagaaatta
    28381 cccttacaag acatatctga tacaacctca cttattagtt tatatgtttc agaagtatat
    28441 aaataaccca tacttacttc caaattctta cctgttttct tagaaaattc taatatgatt
    28501 tccttacata gcgattataa cagtatctta ttagatttca taaaactata catcctcttt
    28561 atatttgaac tttaaatttt tatctattgt ttattaatta actgtatatt cactattttt
    28621 tatcatttat aatattcttc ttattgtctt aaaacaatta taatctgacc ttaatcatat
    28681 attatattat ataaatattt ttaacaaact taataattaa ataactatat tttattatcc
    28741 atcttataaa tgtttataaa ctttccacta ttcttcttca ataaattcaa acaaattttc
    28801 tactgtagta tcgaatactt tagcgatatc catagctaac ttcaaagaag gattatattt
    28861 cccattttct aagtgtacaa tagtctcacg tcttacccca accaatttag caagttcatt
    28921 ttgcgtcata aggagttttt ctctgtattc tttaattttt gttttcatca atattgccac
    28981 ttagttacat atcccccttt tatcaaagta tatatacaac actaatttag ataaagactg
    29041 tataaataaa actgcaatga ggattatacc tgtatctagg tttgatatat ttatattact
    29101 tgtataaggt gttgcaaaga aaatagatat tattactata gaatatatca ttatttttat
    29161 tgcaatctta ttaacctttg atagtataaa ttcagatgat tcatcaacta aatcattata
    29221 ttttttatta taaattgaat aacatccaaa aactattaac caactcaacc ctggaagaga
    29281 agcagtgctt ggacctttaa aaatatactt agtggctaat acagctataa aaaaataagt
    29341 tattacaagc aactccagga taagctttga tcttacacca gtttctgatt tagacatata
    29401 aaatcactcc ttaaaaatta tattctctat actctttaat tttcgttttt atcaatatta
    29461 tcacttagtt gcatattccc tttctatcaa aatgtatata caaaattaat ttagataaag
    29521 attgtataaa taaaatagct aagagaatta cacctatatc caaatttgat atatccaaac
    29581 ctcttgtata atttgctgaa aagaaaacag ctattagaaa aatagaatat aacattattt
    29641 ttgatgcaat atggtcaacc tttaataaca taagctttga cgcctcatca actaaatcat
    29701 tatatttttt attatacatg aagtcacatg cccaaaaagc gaataaaatt ataaaagtaa
    29761 tacaaagatt tccctcttta aaaataaact tttttaataa tgagtctata aaaaaatagg
    29821 atatcataag taactctgaa acaagatttg ttttaacacc aagctctgat ttagacatat
    29881 aaaatcaccc cttaaaagtt atatatttat aacaccttat gttataaata tataactttt
    29941 ccaaataaat tgcaatagaa tattgaaaat attcaataat atcttcataa atatgtttta
    30001 taaaagattt tataataaac attacaataa agtactaaat tagtttattt catctattat
    30061 ctcaatcata tcatttacta actattattc taatgagcat ataacattga aatttataat
    30121 tatacaacct aaatgcctcc caatgtaccc tcaaaaacat catttcctgt aactgaagta
    30181 taattggtat attttatcac tgaaataaaa aaacactagt caataaagac tagtattttc
    30241 aagtatttta tcattctttt tttaattatt ggagacgaat catcagaata atgcccataa
    30301 tttatctata tgcaaattct aaatttaaaa taaagtttca gataaattta ctattctatg
    30361 ctatgataca acagaaaaaa ctgaaatcta tgagtggtag cttcatataa tcctagttcc
    30421 tacagtaatc aaaactgagg tttattctat atgtaactaa tgataaattt tattatagga
    30481 gttattagct ctatttgtgt tactataagt agtaattata tatatgataa ttttttaaca
    30541 cttttctgtt ggctatatag aaagtggttt agaatttaaa tttaaataaa atttaaattc
    30601 accaaaaaga aatctatatt agaaaccact actctattcc accagattgt aattttttgt
    30661 tctttatatc acaatgctat accatattaa attgaaaaat aaattttaaa tttatttgtt
    30721 acaattcatt aattcaaaaa aatattttga gtcatgagtt ttgtaaagat ttagccatga
    30781 atttaatgct tctgattcaa aaacatctaa aagtttcaat acttgaaacg taaattccaa
    30841 cggagcgatt cctgaagccg taactaaatt ttcatcacta actacagatt ccgttttata
    30901 atatttttct ccagagtaat ttggtattac cattttaaga aactctaagt cattacttgt
    30961 atgatttcta acatctaata gccctttttt agctaggcct aatgtagctc cacatatggc
    31021 tgcaactata gaatcttgta ttaaagcttc tttaactttt tctaatatta tctcatgatt
    31081 catggataac caagtatttc cccctggcaa aattaataaa tctttacttt ccatattaaa
    31141 ctcatcaact gtaatttcag gtaaaacttt taacccaccc attgttataa taggattttt
    31201 atcaattcct acagtaatta cttttagtgg ctttaatcct tttttaaaat atcttcctgt
    31261 atttaattca gcagttaaat atgccacttc ccaatctgac atagtatcaa atacatacat
    31321 atatactttt ctatttttca tcttttttac tccttatgta attaatttat atatctaatt
    31381 ataaaataac ttaactgaca tattcagtca gttaagctat taaatttgat aataatcaat
    31441 taattctagt aaatcttcaa ttattttctt cctaaggctt agtggttcaa ttacttgtat
    31501 tgtctttcta taaggtaaaa gtatatttgg tatgtacttg catattactt cctcttcaag
    31561 cataaatatt attttattag gtgttctttc tttcaagtag tgccccaaaa accaatgttg
    31621 gcttaaacta tctaaagaat ctatactccc ttctacaact aattgaatta tattttgttc
    31681 atcttttatt tcaggtataa tcccttttat aaaaaattca cttgctgaaa attctgtcag
    31741 tctgttaaat gtatttgatg tttctatgat ttcactaatc ctatccactc tgaaactacg
    31801 tacctcattt ctcatattac aaaatgcaac tacgtaccaa ttattattcc agtgaacaat
    31861 tccatatgga tttataattc tatattttga ttttgcttta cgacctgcat gatagttgat
    31921 ttctacagat agctccttta atatactatt ctccaatttt ttaagtactg attctataga
    31981 cactttccca attggtttta ttacttcaag ggctcttaag tggtttgtta aaatatcttc
    32041 ttggttttga tttgaataca tttttaactt taatatcgat ttatctaatg cttcgtctaa
    32101 gaagtatcct tcttttttag caaatagagc tgcatgaagt agagattttt gctcttctat
    32161 atcaaaaaaa agaggtgctt tagtaaaatt atttaaaagg ctatacccac cattatgacc
    32221 tgtatctgaa attataggta ctccactcgc agaaagtaca tcaatatatc ggtacactgt
    32281 tcttatattt atttctaatt tttcagctat ctgttttgct gtaatttttt tgtcagagtt
    32341 taacatccac agtatagaca tcatattgtc aattttagcc atgtagtttc acctctttct
    32401 atttgttata taaagttatt atataacttt ttgattgatt ttttacaaaa aaacattagt
    32461 aaactgctaa taatttatac taagatttat ttcaaatatt tatagatagt ctttttctat
    32521 agttaagtta tccattaaat tacatagtag tatatccatt ttagttgata tattttcagc
    32581 tacctccctt gaatattatt acaataagtc taaatcttct ttcttccata aatatattat
    32641 gaggaaagac tatcatcttt aatggtttaa tagtataaaa gatattaatt taaaaaaatc
    32701 tatttttata tcagatgggt attgtttttt ataaaactga aaaaatcacc taatagaatc
    32761 tatgattttt taacataacc tccataaatt agtaccaatt atcacttcaa attcaaattt
    32821 ttatctgaag aatatacaat catatttcca tactcaacat aatattgagt gtttggcttg
    32881 tatttacaaa ttattattct aacaaaaaca actatacata gattaaaatc accttctaaa
    32941 cataattatt aaaatttaat taatgatatc aattccatta atctctttta ataaaaaatt
    33001 ctcttttcta tatgaaaatt atttcaaaac ttttaagtta aatacaaaaa tgactataaa
    33061 tagattttta tttacacaaa tctatttata gtcactatag tttatccaat aaaataataa
    33121 aataaatatg tacacaataa atgttattta cgtcttaagt atttatttgt tagattttta
    33181 attaaaaaaa acaagctaat gaacattaat gctaaatcta gcattatatt actttgatct
    33241 cctgtttgta cattttgacc tacattacta tctaaacttt catctgtatc tatatttata
    33301 tctggttttc cattcccatc tgtatctata tttatatccg gctttccatc cccatctgta
    33361 tctatattta tatccggttt tccatctcca tctatatcta tatttatatc tggttttcca
    33421 tctccatcta tatctatatt tatatctggt tttccatctc catctatatc tatatttata
    33481 tctggttttc catctccatc tgtatctata tttatatctg gttttccatc tccatctgtg
    33541 tctatattta tattcggttt tccatcccca tctgtatcta tatctggttt gctatttcct
    33601 ccattatctg ggtctggttt actatttcca ccgttatctg gatccggttt gctatttcct
    33661 ccattatctg gatctggttt accattgcca ccgttatctg gatctggttt gctatttcct
    33721 ccgttatctg gatccggttt gctatttcct ccattacctg aatccggttt gctatttcct
    33781 ccattacctg aatctggttt gctatttcct ccattacctg aatccggttt gctatttcct
    33841 ccattacctg aatctggttt gctatttcct ccattatctg gatccggctt gctatttcct
    33901 ccattatctg gatctggctt atttagattt ggattacctg tatcataacc tcctgagcca
    33961 gtatctacat ttttatcagg cttccaataa tcatctactc catcattatc tgtatctatt
    34021 gctggacgat tataatctgt atcagggttt ccatctccat tggtatctat atttttatca
    34081 ggtttccaaa taccatctcc atttccacct tctgtactag gcttccattc acctgtatta
    34141 tcagtatcta tgttaatatc tggcttccaa tcaccactcg tatctatatt gatatctggc
    34201 cttccatcac catcagtgtc tatatttata tccggtattc catccccatc tacatctaca
    34261 tttgtcatat ctaactttaa cttcttttca tcctcactac cttcaatgta atttatatca
    34321 tctccaatat attttacagt aatagtttgt tcagtagtaa aaatatgatg attgctatct
    34381 aaaggtctcc attcaataga tgcagtacta taattatgac tctcactacc cttattatga
    34441 gtttttaatt ctactggatc tttatattcc tttccattaa tatagaattg tacgtaacct
    34501 cttggagagg cacaatctgg tccttctttc ttatctggtg aaatagtaac atttaatgta
    34561 aacgtatctg ttggtttaaa aacatcagtt ttagctttag actcttctac aactaatgaa
    34621 gtttctgtat cagcaggaat aatttctgcc ttataatatg ctctatgccc ccaataagct
    34681 tcattaaaag gatatacatt tgaaaactgt gttgatgtaa tgcttaattg atattttcca
    34741 gcatcaactt gctttgtaag gtcatcactt cctaaatttg tatctggttc cattgtttcc
    34801 tcatttaaac gttgagaact aatagtcata aattttttat ctgtgagttt ttcaccttta
    34861 ggagtaatta ttggaatacc cccatctttt aatatccaat ctaaaaataa tttttctaca
    34921 tctccttcaa atggtaatcc atcatatctt ttaataatca aatatggtgg agtatcatca
    34981 atagtaaatt cttcatattg ctttaaaaat tgagaacctg aacctatatc tttaacataa
    35041 aaatcatctg gttttatatt tcctccagta attttatcta taacaactaa attaacagat
    35101 acttcagatc ccttaaattg atcagaaaac cataagtaaa gaaccccttg atcatctgta
    35161 taactaggtg ctccataagg ataaattttt ccatcaatta gcattttaaa atcatcaact
    35221 aatgtagtac taactttatc ttgtcctaaa tactttaaag atattttatt cataaaaact
    35281 tttgttttct tttctaaatc accgtatgca actgctccac ctaaactttt gaatgttgtt
    35341 gcttttaatg aaccacctgt tactataaca tctcctgttg tagcacttag gtcaaaaatc
    35401 caatctccca ctcctgtcat ataaggtaaa atcgttcctc cagttacaag tatacgatat
    35461 cctctatttg ttcctggatt aacacatcca tccccaaatg cactaccatg tgcacctcca
    35521 tatgaatagg aaagtccacc attaattgta atatttccac tataaccaga acctgtgcta
    35581 ggatctacta ctgagggact taatgcattc tttgttgttg tatagtatcc tccacctatt
    35641 ccagctccat ggtatgcacc tttagcagtt atagtaccac cattgattgt gatatctcca
    35701 ccactaccat taccgcctcc acctattcca gctccatatc ctgtattacg actcgattct
    35761 tgacctatag cagttatagc accaccatta ataataatat ttcctccact ttcagtattt
    35821 ccaccgccaa tacctgcaga ataataattt ccaacaactt ttaaacttcc tctattatta
    35881 ctatctagta gtgtaaggcg atctcctttt tttaacttgt ctccattttc tagagttaca
    35941 tctctaggaa tttttccacc ctcaggaact ataaactctc cttcaatatc tctatttaca
    36001 acagaatcat caatagttag agtagtacct tttcctgctc tcatacctgg ataatataca
    36061 tcacctgact ctagcttgtt ttcactacca tcttgtaata taacatgtgc atatgcacca
    36121 ccatctttat ctggtgttaa agtaaatggg ctattagatg cagttttaat atttactcca
    36181 tcaaatatta attcagcttt tacatcttta ggtataacaa tcctatgtgt tgttggagta
    36241 tttggattat tattttgaat tgtcatttgt tttccactaa gaatagttaa aacgccactt
    36301 gcaaatttaa aatcgacatt tatttctcca ccactaattt taaagtctcc tacagatgta
    36361 ggatcttctg cagtggcttc ttccacaact aatgcattca tatcttcttc tgcatttcct
    36421 gcattagaat cttcttctgc atttcctgca ttagcatctt cttctgcatt tcctgcatta
    36481 gcatctcctt ctgcatttcc tgcatt
//
LOCUS       DBGJFF010000029        31712 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.029, whole genome shotgun sequence.
ACCESSION   DBGJFF010000029 DBGJFF010000000
VERSION     DBGJFF010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 31712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31712
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.029"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            161..679
                     /locus_tag="ACOIAF_001467"
                     /pseudo
     CDS             161..679
                     /locus_tag="ACOIAF_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
     gene            1119..1373
                     /locus_tag="ACOIAF_001468"
     CDS             1119..1373
                     /locus_tag="ACOIAF_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668482.1"
                     /translation="MENKDIKLTICIFAFVIGIIVLFCCIGLGQFNMDCIIKQNGGSI
                     ATEQYNIYLEQSISQYRNFGSILVLLGGIGILFDKSVRKY"
     gene            1409..1732
                     /locus_tag="ACOIAF_001469"
     CDS             1409..1732
                     /locus_tag="ACOIAF_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6054 family protein"
                     /protein_id="HGZ2668483.1"
                     /translation="MARDVFSVKMTPLEAQKMVEKNLEADLVSVDIYNLENNKSIIVT
                     IFEKYYARSNSDAGLVVICENTTGKTLVKITSTGSAIGLFQIDWGTGNNLIKRVKKIL
                     IDYII"
     gene            2006..2158
                     /locus_tag="ACOIAF_001470"
     CDS             2006..2158
                     /locus_tag="ACOIAF_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668484.1"
                     /translation="MDNFLFNVLASVTASYIVYLISKLIKKVKSHSTAKSDLKIDINF
                     KYHRKK"
     gene            2426..3289
                     /locus_tag="ACOIAF_001471"
     CDS             2426..3289
                     /locus_tag="ACOIAF_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HGZ2668485.1"
                     /translation="MWILVPIITIVLLIIAVSSMQYILVMIAFLLIIYSFIEKKIVMG
                     LVSVLFFTYSIYLYATCEDKSLIADNKVETVKAQRETVEREKEMERRRIQEEVDKERY
                     IEKHGMEISENDLKVKLEALVPQEYKGKKYELKVGKFKRYSMYFDLTVQNEKFSNSEE
                     CKKFVKEIANDLKKIKISKAYFKFHSKDDGGIYNSVYIDYFRNIQNNVDNVENLEFNE
                     FELKTEEEEKREQEKREQEKIEQEKNSYNNYIQNRVVDPLDRIKKLKELLDSGAITQE
                     EYNKKKKELLE"
     repeat_region   3556..4307
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=3556..3584
                     /rpt_unit_seq="gttttagattaactatatggaatgtaaat"
     gene            4462..4650
                     /gene="srl"
                     /locus_tag="ACOIAF_001472"
     CDS             4462..4650
                     /gene="srl"
                     /locus_tag="ACOIAF_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor Srl"
                     /protein_id="HGZ2668486.1"
                     /translation="MNKIATKSRAEYMKNRRKDKRGFSVLLDKEKLDKFDEVLEEKNL
                     TKKEWLEEKIDEELEQKE"
     gene            4770..5135
                     /locus_tag="ACOIAF_001473"
     CDS             4770..5135
                     /locus_tag="ACOIAF_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HGZ2668487.1"
                     /translation="MKNELMMFEGKEIEVFEFEGRILFNSKDVANCLDIKNVNENIIL
                     MNEKQVVKLRNSDISNTDIRKLNNAGENFLTESGVYKLIFKSRKEEAERFQDWISDEV
                     LPSIRQTGAYITNNAIPKS"
     gene            5188..5583
                     /locus_tag="ACOIAF_001474"
     CDS             5188..5583
                     /locus_tag="ACOIAF_001474"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668488.1"
                     /translation="MLKELLDDAGFDNKSKLLTAKTLYKKAEIDLPIEINEEEHYFDT
                     KQIASKLKIYSKSNKPAQMAVCEIIKKIDLEDGEVKGVWEINGSWTGTVNKYTKSVID
                     KVRTWIEENNRPTKIAGEKKNYYVFYKIE"
     gene            5635..5796
                     /locus_tag="ACOIAF_001475"
     CDS             5635..5796
                     /locus_tag="ACOIAF_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668489.1"
                     /translation="MCENLLDNMYIEKRKEEYRIKLLKIRETDIDIYNKLESIVYKLS
                     EKKLEKNNK"
     gene            complement(6124..6588)
                     /locus_tag="ACOIAF_001476"
     CDS             complement(6124..6588)
                     /locus_tag="ACOIAF_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668490.1"
                     /translation="MDFVEVKSELSDKDLKDNEEALNKYKKYYLKVYKYYSEMDSIKS
                     IQDIEIEQSRLKGQLQKNSNPIFALYISIMAVVFSTTINLTFDLSDITNYFIRMIMTL
                     LFIVLLIFLVNKGILPIAIPYRNNIQFYTTALDVLEDMKNKILRERKYKKRR"
     gene            complement(6724..7041)
                     /locus_tag="ACOIAF_001477"
     CDS             complement(6724..7041)
                     /locus_tag="ACOIAF_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668491.1"
                     /translation="MDFGNINLILIGIIVIIGTTIIYLIKPKTAFCSKKYFNKLESIY
                     GNIDKKKTVKLEVLYRYVTGLEYIAIGLFTRRLDITIIAIILVAIITTALYYLIRKKY
                     ITI"
     gene            complement(7324..7620)
                     /locus_tag="ACOIAF_001478"
     CDS             complement(7324..7620)
                     /locus_tag="ACOIAF_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668492.1"
                     /translation="MYFKTYILKREVNNKQLEITRSFDLDNLICTATEILKDNEIDLI
                     ITDEVNNVVWRNEKSTPPARECLVIAIEVYWWYTSIFLLYHINYVIKKNYLNRF"
     gene            complement(7639..8052)
                     /locus_tag="ACOIAF_001479"
     CDS             complement(7639..8052)
                     /locus_tag="ACOIAF_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668493.1"
                     /translation="MKVKSKRSFIVGIIVCVLCCASLVIYCILKDKRFLISSFLLIVI
                     AIFNFCNAFSRKGIVEELHDSTDERDLYLTMKTSHILVKIMNYTLFTFTFLFIIAYSA
                     WKNQSLLVIAITLCVIEIFLFVAYLLINIFLEKKE"
     gene            complement(8036..8269)
                     /locus_tag="ACOIAF_001480"
     CDS             complement(8036..8269)
                     /locus_tag="ACOIAF_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGZ2668494.1"
                     /translation="MKTKVREFRTNMGLTQQQLADLVHVSNRTIISIEKEQYSPSLML
                     AYRMAQVFGTTIEELCCLKENKEMEDKKHESKE"
     gene            8488..8862
                     /locus_tag="ACOIAF_001481"
     CDS             8488..8862
                     /locus_tag="ACOIAF_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HGZ2668495.1"
                     /translation="MKISKLPEAELKVMRYICEANKVLTSRELVAAMEEKYEWNESTT
                     FTVLKRLERREFLSTEKIGKLTHYNILVKEKKYLRFETKEFLKNIHKNSISSLMSALH
                     GEDDEVDEDKLSELEECFKNLK"
     gene            9474..10166
                     /locus_tag="ACOIAF_001482"
     CDS             9474..10166
                     /locus_tag="ACOIAF_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGZ2668496.1"
                     /translation="MYNILVVDDDKEIVDSIEIYLKSEGFKIYKAYDGMKALEIITEN
                     DIHLILMDIMMPKLDGIKATVKIREERNIPIILISAKSEDTDKIIGLNIGADDYITKP
                     FNLLELIARVKSNLRRYVTLGNYEDTCKEILKSGALELNTLTKEVKIDGENIKMTPIE
                     YKIIELLLENKGRVFSIDEIYEKVWNEESFNSENTVAVHIRRIREKIEINPKEPRFLK
                     VVWGVGYKIEKF"
     gene            10362..12422
                     /locus_tag="ACOIAF_001483"
     CDS             10362..12422
                     /locus_tag="ACOIAF_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HGZ2668497.1"
                     /translation="MEKYRVLKLVSLIIISISFPLTILLVTIGLAEPKYNIQKTSTKS
                     GIFDISDDFYDSNIFDEGTMASLLPKFKLSVGGKLTSKEKGKFQQEGKSIDLSSSDSE
                     YDKIMINEQASEEYLSYLKNIKVYVINTYTGVFYTNTSYASLYDFKQKTKSYCNVEII
                     NKIGKSSYIKTINGEKFELNNLSLELNEVDEGFEAYVSFPEEPTIEDGIVYTNFQIFK
                     QATEKVRLFFVASLSLAIICLLSIILYIKIKGEVLKRNNVFLLIYSKIPMEFNVILVS
                     ISIAILTIYALAVSYHIDVIALVSLSLLIVSNNIFFINVQLSYLENKMCIFKTSIIVR
                     VILWLIKTVREVSNAIKKVSLARKVIVIVIISLALIMVISTRSIGMGLLFSICIFLGF
                     TIYITKRLSYLSYVIEGTERIKKGELDYKIKITGNDNFTSLAENINNIGEGLDRAIEE
                     QVKSERMKSDLITNVSHDLKTPLTSIINYVDLIKKEESIQPEYINDYINVLESKSKRL
                     KLLIEDLFEASRVSSGNIELQISKIDLVQLLRQSIGELEEKLSKNNLYLKLNVPNDKV
                     YIWADGRRMYRVFENLLSNIAKYSLEETRVYIDVYDYGENVKVTMKNISSYELNFDPS
                     EIMERFKRADESRNTEGSGLGLAIARDLVALQGGKFYIEIDGDLFKANLEFQKYEEEI
                     TNME"
     gene            complement(12628..12951)
                     /locus_tag="ACOIAF_001484"
     CDS             complement(12628..12951)
                     /locus_tag="ACOIAF_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HGZ2668498.1"
                     /translation="MNNNYIFFIIIGMYIVTYLPRVLPMLIFSKKEMPDFLKKIMKFI
                     PVAILTSLTAKDVFFNGDSLYLSIANPKIISFMIVLLVAYKFKSIGISIITGVISIYL
                     FGIIL"
     gene            complement(12944..13645)
                     /locus_tag="ACOIAF_001485"
     CDS             complement(12944..13645)
                     /locus_tag="ACOIAF_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HGZ2668499.1"
                     /translation="MERTQQLKESFIASIPIFLGYIPIGIVGGILLQKSGLSPFQIAL
                     MVTLVFGGSSQFIAASMISTGASVTSIVLTTFIVNLRHFLMSSNLNMYIKNKSPKFIL
                     PFCHTITDETFAVNYEKFTAGNWSDRNAIYLNFFCLISSVLANFIGAFLGESISIDSS
                     ISGFILTSMFIALIVSQIKNKIYIVVFISSIIISVILYALFKSNLVIIVASILASLIG
                     FFIEESYCKKEESYE"
     gene            complement(13923..15308)
                     /locus_tag="ACOIAF_001486"
     CDS             complement(13923..15308)
                     /locus_tag="ACOIAF_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HGZ2668500.1"
                     /translation="MPINSFENYPMNWKPKRPSKGQILYKALAEQLEQDINNGFLLPG
                     TKLPPQRELADFLDVNVSTISRAFKICEKKGLISGVTGSGTFVSYDTRSNLFLMSSNN
                     KITFIEMGTMNPDFTLEEMNTLFKHIIKEIDFKTIFQYGQRDGAKWQKEAIAKLIYKA
                     GLETTSDSLLPASGGQNAIVAILAGLFQHGDRIGVDPLTYPGIKTAAKMLGIQLIPIK
                     QEDNEISEEGLLYACKNENIKGLYIIPDYQNPTTHIMSQNGRKMIANIASKYNLIVIE
                     DAIHSLLNETHLNPVASYLPNQTIYITSLSKIIAPSLRLAYISTPKQYREALSSALYN
                     INLSQSYFLTEIAYRMITSGEADKLINARRKSARRRNKIINQYLSGYNLLGNEECIFR
                     WLILPDGIMAEKFEIQALKEGVQVYASERFAVGKEKPISAIRIAVCATESTEELKAGL
                     SVLKRLLEEKK"
     gene            complement(15754..16176)
                     /locus_tag="ACOIAF_001487"
     CDS             complement(15754..16176)
                     /locus_tag="ACOIAF_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HGZ2668501.1"
                     /translation="MNTFKTEVLLKYSAKDIFNIFTKSARLNFPNFNEKKAVGTFVQQ
                     KNNKRFKVEITNFEKNRVYEIKTSNKRESTITRYELVHIDLENTKLIFIESESKRNIF
                     GKINSALVRVLFGKRQPEEFNKFIKKLEMELENNSVNL"
     gene            16654..19557
                     /locus_tag="ACOIAF_001488"
     CDS             16654..19557
                     /locus_tag="ACOIAF_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HGZ2668502.1"
                     /translation="MELRDKLVNIINTENKKDPLTDVQIAKFLSTTRENITNLRKELN
                     ISNSRQRRYPYLKSAISAILQKNKNISISEITRELMTEGFNISRRVVEELLPKESLEM
                     DVVEESEEESNDPFETLIGNKGSLRNAVEQAKSAILYPPKGLPTLIIGESGVGKSLFS
                     RHMYEFAKQKKIVKESSNFVVFNCADYSDNPQLLLSLLFGYKKGAFTGAEYDTPGLVE
                     EADEGVLFLDEIHRLPPKGQEILFSILDRGKFRRLGETNAERKVSIMLIGATTENVET
                     NLLLTFRRRIPMLITLPPLHDRLLKEKIDLIYNIFQQECNRINAKIFVDKNVIEILAL
                     KKFSGNIGQLQNMIQVLCARAFMKFINSKDEDSESIVVVDINEVLKLKDSFKDAAFQE
                     IEYTEIRKYLKNAIFIPFNLEESSVKGSLLSDEYILPEDIYKTIEKKYYDLKSLDIED
                     IDIENILWTFILNRFADLKFNLDSNEEIFSMSDLNSFVSDSLVKLVKELMNDIVEKNM
                     NLEVNKNMFKYLAIHLEEAIKRIKLNQKIINVNLEKIKIDFSKEYEISKSFAIKIEES
                     IGIKISDDEIGFITLYIKSALKNEVKKDKVGLIIISHGRIATETVNVVKELLGVKFPV
                     AIDMPLDEKPINIYNKAVELSKIIDQGKGILFLVDIGSLTNIGQIVNKRTNISTKTID
                     RVDLLMALEATRKVSMGEEELDEIFFSLLKDRMGYNYELEKHIDKSNAIITLCLTGEG
                     TAKYISKTLEEKYDNTKCYQMSALDENLFCKIENLKETNNILAIVGTINPKIPGINFI
                     PYNKELFKNLDIYLSKSKQQDDRPVKSYERMLDEDLVIFEPDIYFKKDLLEYVCSILI
                     NKEYVEKDYLDSVLHREEMLPTYSKGAIGVPHGDSSTVNSTRFVFVKLKNPIDWGVGN
                     VNFIFMPVFQANDKEIVKNVLEILKDAEFMNNANKCFDKDAFRRIIFDKFKHL"
     gene            19590..20036
                     /locus_tag="ACOIAF_001489"
     CDS             19590..20036
                     /locus_tag="ACOIAF_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGZ2668503.1"
                     /translation="MNVDLISLGLESNDYESVIEELGSIMCKKEYVKETYINAVLERE
                     RTLPTGLDIGEMCVAIPHTDSKHVNESNVAVGILKNPVKFNSMIDPKDRLDVELVFLL
                     AVKNPDSQVKLLKDLMSVFQNIKLLKNIKNASTKEEVAKLLDFIEI"
     gene            20159..20461
                     /locus_tag="ACOIAF_001490"
     CDS             20159..20461
                     /locus_tag="ACOIAF_001490"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGZ2668504.1"
                     /translation="MKQFNVLSVCGSGTVTSSMVAGKLKEVLGNKGISITTTEARPTE
                     ALNLAQSGRFDFITFTSPLQTGDYGIPTINAFACLTGIGEEAFFEEVLTVIEEIDK"
     gene            20642..22048
                     /locus_tag="ACOIAF_001491"
     CDS             20642..22048
                     /locus_tag="ACOIAF_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HGZ2668505.1"
                     /translation="MLEFMKSIIDTFGSAIIVPIIIFIIAKIFKVTTKKSFLSAVYAG
                     VALQGFTLILNSFTPIITPVINRMVESTGVNLPVFDVGWQATSLVAFSTSAGMIYLGL
                     GILLQTILFLIKWTDVFQPSDLWNNYSYMVWGAMVIGVTGNFPLGIACMVLLNLYSLL
                     ISELVAKRWSSYYRYPNCTIIAMHNVEASVFAVFADPIYNKLGLNKIKLNPKELEKKL
                     GFLGEPITLGLFLGMFIGILGNMTRINTMEAWGEIMKVGISTSAVMAIFPKVASMFAQ
                     AFAPITEAARKIMQKAGNREWYIAVNDAVGYGEPATLISGLILIPIMLVIAMVLPGNK
                     VLPVVDLLAIPYMVQGLVAIHNGNIPKVLVSGIIWFGLGLYVCTSTAPLFTDMATNIG
                     VAIPAGAMLITSFNILGKPLMGLVFFAFLSANPIYIGLSVVIYFVLWALFRKNKTSIL
                     DYLEKQALKNVEEEPVAV"
     gene            22392..23450
                     /locus_tag="ACOIAF_001492"
     CDS             22392..23450
                     /locus_tag="ACOIAF_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HGZ2668506.1"
                     /translation="MGNKMRASVLYNVGDVRYEMVDIPEITDTQVLVNVKYVGICGSD
                     LPRSMVSGLSGNTKYPLILGHEFSGEVVKIGEKVKHINVGDRVAVAPLVPCGKCDYCN
                     EGNFGLCDDYNIIGTRVNGAFAEYVRVPEEHILKLPDTLDYETAAGIEPATIAYHGIS
                     KSNIRVGDSVVVLGCGPIGQFVIQWAKVFGASKIIAVDIFDEKLELSKLLGANYILNS
                     KEVNVIKEIKKITNGGADVVIETAGSRFTQEQSLFVAKKRGNIVFVGISHTELPLSAD
                     ATECILRGELTLKGSWNSYTSPYPGRAWTATLDFMEKGDIIFKPMISDKIGLNEVGDF
                     LSKMSKREINFNKILVEI"
     gene            23549..24235
                     /gene="araD"
                     /locus_tag="ACOIAF_001493"
     CDS             23549..24235
                     /gene="araD"
                     /locus_tag="ACOIAF_001493"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HGZ2668507.1"
                     /translation="MLENLKEEVLKANLELPKKKLITYTWGNVSGIDREKGLVVIKPS
                     GVEYSNMTVEDMVVVDLEGNIVDGKLRPSSDTPTHLVLYKEFEELGGIVHTHSSWATI
                     WAQIGKSIPSCGTTHADYFYGSIPCTRKMTREEICNEYEKETGNVIIEEFMGRNPIHC
                     PGIIVNDHGPFTWGKDANEAVHNAVVLEEVAKMAYYTELMSPDNIMDKVLMNKHFSRK
                     HGKNAYYGQK"
     gene            24253..24900
                     /locus_tag="ACOIAF_001494"
     CDS             24253..24900
                     /locus_tag="ACOIAF_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HGZ2668508.1"
                     /translation="MLDSLKGKVVEIAIKAEHYGLCKEKTGNFSIRDKDTGYILITPS
                     GIGREELKVEHICVLDLDGNVIEVEKGIKPSSEVLMHIEIYKTRENINAISHTHSLYA
                     TAFSVAKKPIIPIVYESVNYGGYVDIASYERPGTLKLAKSVSKLLIDTDACLLERHGL
                     TTVGNDLDEALLKSRYVEEVAEIYYRSLVLNQFKELEVVDVDEFKSWKYPEEIKF"
     gene            complement(25073..26440)
                     /locus_tag="ACOIAF_001495"
     CDS             complement(25073..26440)
                     /locus_tag="ACOIAF_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HGZ2668509.1"
                     /translation="MNLIDILNKVDAFIWGPPLLVLLVGTGILLTVKLGVVQITKLPR
                     ALKLIFSAENKGSGDVSSFAALCTALAATVGTGNIVGVATAIKAGGPGALFWMWIAAF
                     FGMATKYSEGVLAIKYRTKDKNGQVSGGPMYYIVNGMGEKWRPLAIFFAISGILVALL
                     GIGTFTQVNSITDAINNSFGIDPRITGVVLAVFVALVVFGGLKSISNVATKIVPFMAV
                     IYVVICGIILISFWNKIPETFMLIIKSAFTPTAATGGFLGATMSLAIRNGIARGVFSN
                     ESGLGSAPIAAAAAKTEWPAEQGLISMTGTFIDTIIICTLTGFSLVISGVWCSDLNGA
                     VMTQAAFNGAIPTFGPILLTVSLTLFAFTTILGWSYYGERCFEFLFGVKGMNGYRTVF
                     VAMVLLGAFLKLEVVWIIADIVNGLMAIPNLIALLALSPIIVSETKKYFEHINSPENQ
                     IKKNA"
     regulatory      complement(26630..26718)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            27146..29266
                     /locus_tag="ACOIAF_001496"
     CDS             27146..29266
                     /locus_tag="ACOIAF_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HGZ2668510.1"
                     /translation="MGKILVLAEKPSVGRDIAKVLGSKNEKNGYIEGPKYVITWALGH
                     LVTLADPESYGERYKSWSLEDLPILPKHLKTVVIKKSGKQFNTVKSQMNRNDIDEIVI
                     ATDAGREGELVARWIIEKSQVKKPIKRLWISSSTDKAIKDGFAKLKSGKEYENLYYSA
                     IARAEADWIIGINATRALTTKYNAQLSCGRVQTPTLAMLLKREEEIRNFKPKEYYGLE
                     LIATKGNSDIKFIWNDKNNNSSTFSKEKIESTLKKVKGVDIKISEVNKSYKKKYSPAL
                     YDLTELQRDANKMFGFSAKETLSIMQKLYEHHKVLTYPRTDSRYLTSDIVGTLKDRIK
                     AVNISDYSKICNRLLKSNIIANKSFVDNSKVTDHHAIIPTEQKVFMSDFSDKERKIYN
                     LVVKRFLAVLSPPFEYEQTTISATVEGEIFKAKGNKVINLGWKESYKDIEDEDEYESI
                     PNVLEKDVLKVKGLKITSGKTNPPPYLNEGTLLTAMEKNGLGTVATRADIIEKLFNSF
                     LMEKKNKDIYITSKGRQLLDLVPVDLKTPELTASWEKKLLDISKGKLSKNTFINDMKD
                     YSKDIVLEVKNSDNKFKHDNLTKNRCPECGKFMLEVNGKKGKMLICEDRECNTRKTIS
                     QVTNSRCPNCHKKLELRGEGEGRIFTCSCGYREKLSSFNKRKQEEKQKASKKDVNQYL
                     KNQNKNQETFNNPFAEALAKLKNK"
     gene            29370..30623
                     /locus_tag="ACOIAF_001497"
     CDS             29370..30623
                     /locus_tag="ACOIAF_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliS"
                     /protein_id="HGZ2668511.1"
                     /translation="MDLSGIFKYYCKECENTWNNSSVELFENIETYSKDSQKKREKEL
                     DKLLNTISVHLERYPSDAVLRKMWVKKGEVFLQKTLEKENIFKLEKMDVEDRKKFLDI
                     TKQFIRDARKFDDDLPIGDIMQAMRNVWISNALQLLFGKEVYYSKANFAYSMLYPYTD
                     NYLDNTNIDKNDKILFNNWLEKRLLGEHIKSKDYHESKVSQMIDYIESVYPREKFTEV
                     YESLLLIFKSQVNSLKQHGKENYLCKEDLLSISIEKGGSSVLVDGYLISGLMTKEEIE
                     FCIGYGFLLQISDDLQDIKEDLKYNHKTIITEMSKEGTLDKVVNKLINFTIELIDSFK
                     INNKNKSVITMIKNDCLMLILFSVVYNAEFFSVGYIKEVEKFIPYTIDYSLEIEEKIK
                     EKFKNIDVLNNENEYKEMIDIICAE"
     gene            complement(30777..>31712)
                     /gene="ytvI"
                     /locus_tag="ACOIAF_001498"
     CDS             complement(30777..>31712)
                     /gene="ytvI"
                     /locus_tag="ACOIAF_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HGZ2668512.1"
                     /translation="IASILRPIIRLITNNTNLNRTLISIVILLIFYGLCIFLLVSFGV
                     KIFASVSDVFFRLPEIYKSSIQPTLNTLFSKIDASTPNVNLALILGWDNISQSMMSLV
                     ASISTNALNAIASIASKTPAFMLKLIITLIASFFFTFDYQKIVNFILKQFPEKSQFMI
                     INIKNSSINALLKLLKAYAILLSVTFIELLIGLTILKVENAFTISVIIALVDILPVLG
                     TGSILTPWMIISLINGNINLAIGLLILYIIITVVRQILEPKVVGHQIGLYPLITLMCM
                     FVGAQLFGIAGLFGFPIAATIIKNLHDNGIITAFK"
BASE COUNT        12597 a         3510 c         5108 g        10497 t
ORIGIN      
        1 tttaatagaa tatgtatagt atatagtaga tagaatgtat aaaaataaaa actgttcaat
       61 aagaattaat atacattaat tcagctgaat ccaatcaaaa tacttaaata aaagtaatct
      121 gaaaataaca taattcaaat caatttattc aaataaacct caatttatct caaatcttaa
      181 gaagtcatta aacaaacttg ctaaattaca aagagaatta tcaagaaaaa caatcggtag
      241 tttaaataga aataaagcaa gattaaaagt tgcaagactt caagaacata tagctaatca
      301 aagaaatgat tttctacaaa aactatctac taaattaata aaagagaacg atattatttg
      361 catagaagat ttacaagtaa agaatatgat taggaatcgt aaactatctc gctcaatttc
      421 tgatgtgtca tggtcagagt ttattcgtca attagaatac aaggctaatt ggcatggtag
      481 acaaattgta aaggtgggta aattctttgc aagttcacaa atatgcaata aatgtgggta
      541 taaaaacgag gaagtgaagg acttaaatat aagagaatgg atttatccta gttgtaatga
      601 aactcacgat agagatataa atgcaagtat aaatatatta aaagaaggat taagactaat
      661 aacaattcaa aataaataac gtatgtaaga accgtaggaa ctacggggat agcctgtgga
      721 gaattagtaa gacatatgtt agtatgcaaa attctatgaa gcaggaaacc tgtgacttta
      781 gtcatgggag gttcaggcta gtattactca ttattgtatt caaaagctaa agtggaaacc
      841 tagtgaatat atgaatttag aagttaatga gagagcatta gcagccgact caatacttat
      901 taaaattgaa gatgaagaag aagcaatgaa agaagctgaa agagagaaaa agagaggtag
      961 aagaaggtag aatttaaagt taaaatagtt acattgctaa tatatgaaaa tgttacaatt
     1021 ttggaaatag atggatatat atgttgtata gtataatgta gataatatat caccaagtaa
     1081 aaaacaacaa ataaatctaa attatgaggg gtgtatttat ggaaaataaa gatattaaac
     1141 ttacaatatg tatatttgct tttgtaattg gaataatagt gttattttgc tgtataggtc
     1201 taggtcaatt taatatggat tgtattataa aacaaaatgg tgggtcaata gctaccgaac
     1261 aatacaacat atatttagaa caatctattt cacaatatag aaattttggt tctatactgg
     1321 ttttactagg aggaatagga atactgtttg ataaaagtgt cagaaaatac taaaaaagta
     1381 tatttttatt taggatgtga tgaatgatat ggcaagagat gtatttagtg taaagatgac
     1441 accactagaa gcacagaaaa tggtagaaaa aaatttggaa gcagatttag tttctgttga
     1501 tatttataac ctagaaaata ataagtctat aatagttaca atctttgaaa aatattatgc
     1561 aagaagcaat agtgatgcag gtttggtagt gatttgtgaa aatactacag gaaaaacact
     1621 tgttaaaatt acctcaactg gtagtgcaat tggactattt cagattgatt ggggaacagg
     1681 taataatttg attaaaagag taaaaaaaat acttattgat tatattattt aattatttta
     1741 tgtagatatt cttaggttgg tatggaattt gtactaacct attaagtaat caataaaact
     1801 aaataaacat agaaagcact tacaaatatg taggtgcttt ttattgaaaa aatagaaaat
     1861 aaaataaaaa gtaagatata ggaaaaatat gtaagaatta tatattataa taattgtagc
     1921 aagaagatgt aatctacaat ttatagagtg gagttcatgt attaaaaaat tatcctccca
     1981 acgataagaa gggaggtgaa tatacatgga taatttttta tttaatgtgt tagctagtgt
     2041 aacagctagt tacatagttt atttgattag caagttaatc aaaaaagtaa aaagccactc
     2101 taccgcaaag agtgacttaa agattgatat aaattttaaa tatcatcgta aaaaataact
     2161 ttcagttata gaacttcact ctaccgcaaa tagattgtag ttcttcttgc ttttattata
     2221 ccacaaatta gaaaaaatat tgcatataat attttttata gtcaataaaa atatgaaatt
     2281 ttttatacac aatgatgatg aatctaacta gatgaataaa tttaattgta aataagaaag
     2341 tacttactta aatggtaggt gcttttgttt tgccaaatta tgctataaca ttgagtgaga
     2401 tgagtaattt gtggggaatt ttacaatgtg gatattagta ccaataataa caatagtatt
     2461 acttataata gctgtttctt ctatgcaata tattttagtt atgatagctt ttttgttgat
     2521 tatatattct tttatagaaa agaaaatagt tatgggtctt gtgtcagttc tttttttcac
     2581 ttattcaatt tatctttatg caacttgtga agataaatcc ttgatagcag ataataaagt
     2641 tgagactgtt aaggcacaaa gagaaactgt agaaagagaa aaagaaatgg aaagaagaag
     2701 aatacaagaa gaagtagaca aagaaagata tattgaaaaa catggaatgg aaatatcaga
     2761 aaatgattta aaagttaagc tagaggcttt agtacctcaa gaatataagg ggaagaaata
     2821 tgaattaaaa gtaggaaaat ttaaaagata tagtatgtat tttgatttaa ctgtacaaaa
     2881 tgaaaaattt agtaattctg aagaatgtaa aaaatttgta aaagaaattg ctaatgatct
     2941 taaaaaaatt aaaataagca aggcgtactt taagtttcat tcaaaagatg acggtggtat
     3001 atataattct gtttatattg attattttag aaacatacaa aataatgtag ataatgttga
     3061 aaatttagaa ttcaatgaat ttgaacttaa aaccgaagaa gaagagaagc gagaacaaga
     3121 gaagcgagaa caagagaaaa tagaacaaga aaaaaacagt tataataact acattcaaaa
     3181 cagagtggtt gacccactag atagaataaa gaaactaaaa gagttgctag attcaggagc
     3241 aattacacag gaagaatata ataaaaagaa aaaagaatta ttagaataga taatatagta
     3301 agcacttaca agtatgtagg tgcttttaaa tttacaaagt attccatttt aattttatag
     3361 tttagatttt atgatataat aaaaatatag aagttttgca gtgtgcgata tttgttacaa
     3421 agtagggctt aatacttgaa atctaagatg ttgagggtgc gtgataagtg ttatcaattg
     3481 cactattgcc cgctcactgc aattttaaga gtattgtata tatgtaggta ttggaaatgc
     3541 taagtttatt ttggggtttt agattaacta tatggaatgt aaattaataa gggtgttata
     3601 aactcatttt tttaacttgg gttttagatt aactatatgg aatgtaaata aatgaataaa
     3661 tcttgtgtca tcacacacca gacctgtttt agattaacta tatggaatgt aaatttgcta
     3721 caacgatata gattaaattt aggaggatac gttttagatt aactatatgg aatgtaaata
     3781 gtttaagtga tgcttatttt gtaaaaagta cgacaggttt tagattaact atatggaatg
     3841 taaattttat cttttttaaa gactatagta aaaaaataat agttttagat taactatatg
     3901 gaatgtaaat aggcaaatta gtcaagtgct ttggatataa aaagtttctg gttttagatt
     3961 aactatatgg aatgtaaata aattcgacgg agaactttta tctaataaag aaaaacgttt
     4021 tagattaact atatggaatg taaatgagaa aatttcagaa tggtttaatg cagtagtaga
     4081 aagttttaga ttaactatat ggaatgtaaa tatgcacaat tcaaattata aagatgactt
     4141 gcagaaagtt ttagattaac tatatggaat gtaaattatt cttttgccaa ctttattcca
     4201 ttattcaaat cttgttttag attaactata tggaatgtaa actgctgtga tttcactaat
     4261 aaaacaatta ataattgtgt tttagattaa ctatgtggat tcaaaattaa ataaacaaag
     4321 aaagcactta caaatatgta ggtgctttta ttctgctcaa aattggtcgg ttgggtaaaa
     4381 taattagaaa aaattagtaa aaacctattg actgtaactc gttacaatat tgttattaat
     4441 gtaacgagtt acagaaaaga ggtgaataaa atagcaacta aaagtagggc agagtatatg
     4501 aaaaatcgtc gaaaagataa aagaggtttt agtgtacttt tagacaaaga aaagttagat
     4561 aaatttgatg aagtattaga ggaaaagaat ctaaccaaga aagaatggct agaagaaaaa
     4621 atcgacgagg aactggaaca aaaggaataa aaaataaggg tcactcccac cgaccaaagt
     4681 ttgagtaacc cctatgatgt atactatcgt atatcaatta taatatatgt cattccttaa
     4741 aaaaatcaat tattaaggag tgtaatatta tgaaaaatga attaatgatg tttgaaggaa
     4801 aagagattga agtatttgaa tttgaaggaa gaattttatt caactcaaaa gatgtagcaa
     4861 attgtttaga cataaaaaat gttaatgaga atataatact tatgaatgaa aagcaagttg
     4921 taaagttaag aaattcggat atcagtaata ctgacatccg aaaacttaat aatgctggag
     4981 aaaattttct tactgaaagt ggagtgtata aattaatctt taaatcaaga aaagaagaag
     5041 ctgagagatt ccaagactgg ataagcgatg aagttttacc aagcataaga caaactggtg
     5101 catacataac taataatgca atcccgaaaa gttgagagaa aaagcaagtg agattgaaaa
     5161 attacagttg cttataacag tacatctatg ttaaaagaat tgctagatga tgcaggcttt
     5221 gacaataaat caaaactatt aacagctaaa acattatata agaaagcaga aattgattta
     5281 ccaatagaga taaacgaaga agaacattat tttgatacaa aacaaatagc atctaaactg
     5341 aaaatatatt ctaagagtaa taaaccagct cagatggctg tttgtgagat tattaaaaag
     5401 attgatttag aagatggtga agttaaaggt gtttgggaaa ttaatggttc ttggactggt
     5461 actgtaaata aatatacaaa gagtgtaata gataaggtga gaacttggat agaggaaaat
     5521 aatagaccta ccaagattgc aggtgagaag aagaattatt atgtatttta caaaattgag
     5581 taaatttatc agttgtatta aataatttag tttagttttg gggggattaa tacaatgtgt
     5641 gagaatttac ttgataatat gtatattgaa aagagaaaag aagaatatag aattaaactt
     5701 ttgaaaataa gagaaacaga tatagatata tataataagt tagaaagtat agtatataaa
     5761 ctttctgaga aaaaattaga gaaaaataat aaatagacat ataaagcact tggatattct
     5821 actgtttcaa gtgcttgttt ggtataaaat ggtataatgg agatagagaa ttatattaac
     5881 caaaaaatgg agaaaataga gaagatgatt tagttgacgc tttatcagta ggagtacctg
     5941 caggtaagac agaatctcta gtacgattag ttggagatgg aataagtgat actcaaaaaa
     6001 gtttaataaa taaaagagaa tttagaaata taactcaaat tggtggaaat ggaaatgaga
     6061 aagcatttag tgaagtagaa aacttagtta aatagattag aaggtagcaa ctagagttgg
     6121 ttgttacctt ctttttttat actttctctc tctaagtatt ttatttttca tatcttctaa
     6181 tacatctaat gcagtagtat aaaattgtat attatttcta taaggtatag ctattggaag
     6241 aatgcccttg ttaactaaaa aaataagaag tacaataaat aaaagtgtca taatcattct
     6301 aataaaataa ttagtgatat ctgataaatc aaatgttaag ttaattgttg tagaaaatac
     6361 aacagccata atagatatat acaaagcaaa aatggggtta ctattttttt gtaactgtcc
     6421 tttaagccta gattgctcta tttctatatc ttgaatactt tttatggaat ccatttcact
     6481 ataatactta taaactttta aataatattt tttatacttg tttaaagctt cttcattatc
     6541 tttaaggtct ttgtcactta actctgattt aacttccaca aaatccataa aatccctcct
     6601 acaatgttat ataatatact ttaatttata gcatatttaa gaacttatat cagcaatttg
     6661 tcgaacgatt ttagctaaaa atagagtata atgataggct tattatttat aagatattat
     6721 tttttatata gttatatatt tctttctaat tagataatat aatgctgttg ttataatggc
     6781 tactaatatt attgctatta ttgtaatatc taatcttctt gtaaataagc ctattgctat
     6841 atactcaaga cctgttacat agcgataaag tacttctagt ttaacagttt ttttcttatc
     6901 tatatttcca tatattgatt ctaatttatt aaaatatttt ttactacaaa aagcagtttt
     6961 aggtttaatt aaatatatta ttgtagttcc tattataact attattccaa taagtataag
     7021 attgatattt ccaaaatcca tagcagatac ctcctattaa atttgctttt cttaatcaaa
     7081 gtatatcagt aacacaagta caaagagtat ctaaaatatt acaaatatta acaggataga
     7141 taaaactata gtaaaaattg taataataaa gcgaacgaaa attatcatac taaaacataa
     7201 gacatgttat aattgtatta gatagaagat taacatacac caaagcactc cttataataa
     7261 agagtgctta tttttttgaa attcatcaat atataaacta tcaagaacct tattcaacac
     7321 accttaaaat cgatttaaat agttcttttt gataacataa tttatatgat ataataaaaa
     7381 aatagaagtg taccaccaat acacttctat agctataact aagcattctc ttgctggggg
     7441 agtgcttttt tcatttctcc aaacaacatt atttacttca tcagtaatta tcaaatcaat
     7501 ctcattatct ttcaaaatct ctgttgcagt acaaattaga ttatctaagt caaaacttcg
     7561 agttatctct agttgtttgt tatttacttc tctttttagt atataagttt taaaatacat
     7621 ttatatcacc ccttgtattt actctttttt ctctaaaaat atattgataa gtaaatatgc
     7681 aacaaataaa aaaatctcaa tgacacatag tgttatagct ataactagaa gtgattgatt
     7741 tttccatgca ctataagcta tgatgaaaag aaatgtaaaa gtaaataatg tataattcat
     7801 aattttaact aagatatgac ttgttttcat tgttaagtac aagtctcttt catctgtact
     7861 atcgtgtaat tcttcaacaa tgccttttct tgaaaaagca ttacaaaaat taaagattgc
     7921 tattacaatt aaaagaaatg atgaaattag gaatctttta tcttttaaaa tgcaataaat
     7981 aactaaacta gcacaacata acacacaaac tataataccc actataaaac ttcgtttact
     8041 ctttactttc atgtttttta tcctccattt ctttattttc ttttaaacaa catagttctt
     8101 caatcgttgt accaaacact tgtgccatac gataggcaag cataagagaa gggctatatt
     8161 gctctttttc aatagaaata attgttctgt ttgatacatg gactaaatca gcgagttgtt
     8221 gctgtgttag acccatattt gttcgaaatt ctcgtacttt tgttttcatt ccataacctc
     8281 tttctatatg aagctaactt catgtgaagt taacttcata atatcctaaa atgagtaaaa
     8341 tgtcaatctc attagataag tattataaaa cctaaaagaa taatatggta aaaattggat
     8401 ggaattattt accttaattc tcttgtttgt aaaactttat tgatatagaa tttaacatat
     8461 acataaatcg gcaaaggggc gcgaattatg aaaattagta aattaccaga agcggaatta
     8521 aaagttatga gatatatttg tgaagctaac aaggtattaa cttctaggga attagttgct
     8581 gcaatggaag aaaaatatga gtggaatgaa tcaactacat ttacagtttt gaaaagatta
     8641 gagcgaagag agtttttaag tacagagaaa ataggcaaac ttacacatta taatatacta
     8701 gtaaaagaga aaaaatatct aaggtttgaa acaaaggaat ttttgaagaa catacataaa
     8761 aattcaattt caagtttaat gtcagcgtta catggtgaag acgacgaagt ggatgaagat
     8821 aaattaagtg aactcgaaga atgttttaag aatttgaaat aagaaaatga caaattgata
     8881 cataataaat aaaatgaagg tatgaaatta taactttttc taccttcatt ttgtttatta
     8941 ttctgaacat gttaagaaaa tgaaataagt ttgtatttta gttatataaa ttttaataga
     9001 atatgtacag tatatagtag atagaatgta taaaaataga atttaaataa gtagctatta
     9061 ttgcagtgaa atgaatgttt ctaagtatat ttgggtggaa gaatggtgga agaaaattag
     9121 tgaaatgtaa tttttccacc attcttccac ctagaaaata gaaactgttt aataagaatt
     9181 aatatacatt aattcagctg aatgcaatca aaatacttaa atgaaagtaa tctgaaaaca
     9241 acataattca aatcaattta ttcaaataaa cctcaattta tcttaaatct taagaaatat
     9301 ttaagaatta ggataatatt ttcttaagat ttatatttta ttatatagtt ataagggaaa
     9361 gttacaaaaa tgtaagccaa agtaatataa caatttagtc attgttgaga aagggagtga
     9421 aagatattgt ataatataga taagtattat tatacaatag aggaagtgaa aatatgtaca
     9481 atatattagt agtagacgat gataaagaaa ttgttgattc gatagaaata tatcttaaat
     9541 cagaaggatt taaaatatat aaagcatatg atggaatgaa agctttggaa ataataacag
     9601 agaatgacat tcatctaata ctaatggata taatgatgcc taaattagat ggaatcaaag
     9661 caactgtaaa aatacgagaa gaaagaaata ttccaataat tttaatttca gcaaaaagtg
     9721 aagatacaga taaaataata ggattaaata ttggagctga tgattatata acaaagccat
     9781 ttaatctatt agagcttata gctagggtta aatcaaactt aagacgatat gtaactttag
     9841 gtaattatga agatacatgt aaagaaattc taaaaagtgg agctttggag ttaaatacac
     9901 taacaaaaga agttaaaatt gatggtgaaa acataaaaat gactccaatt gagtataaaa
     9961 taattgaact tcttttagaa aataaaggac gagttttctc aatagatgag atatacgaaa
    10021 aagtatggaa tgaggaaagt tttaattcag aaaatacagt agcagtacat atcagacgaa
    10081 taagagaaaa gattgaaata aatccaaaag aaccaagatt tttaaaggtg gtgtggggag
    10141 ttggttataa gattgaaaaa ttttagtgga aaaattataa aagaaaagag tttaatattt
    10201 aattgcattt tgattttagt catttttaca tttacacaaa tgttaggaat tttaatatgt
    10261 tttaattttt tctctgagag tttaatttac tctatgggga ttatgctttt cttaaacttt
    10321 attttatata aaatactgac aatgtagatg aggtgggcaa cttggagaaa tatagagtat
    10381 taaagttagt ttcattaatc ataatatcta tctcatttcc attaactata ttgttggtga
    10441 caataggact tgctgagcct aaatacaata tacaaaaaac ttctacaaag agtgggattt
    10501 ttgatatatc tgatgatttt tatgatagta atatatttga cgaaggaaca atggcaagtc
    10561 tattacccaa gtttaagcta tctgtaggtg gtaaattaac tagtaaagaa aaaggtaaat
    10621 tccaacaaga aggtaaaagt atagatttaa gtagtagtga ttctgagtat gataaaatca
    10681 tgattaatga acaagcttct gaagaatatt taagttatct aaaaaatata aaagtgtatg
    10741 ttattaatac atacacagga gtattttata caaatacgtc atatgctagt ttatatgatt
    10801 ttaaacaaaa aactaagagc tattgtaatg tagagataat aaataagata ggaaagagct
    10861 catatattaa aacaataaat ggagaaaaat ttgaactgaa taatctgagt ctggaactaa
    10921 atgaagtaga tgaaggtttt gaggcatatg tatcttttcc agaagagcca actatagaag
    10981 atggaatagt atatactaat tttcaaatat ttaaacaagc tacagaaaaa gtaagattat
    11041 tttttgtagc tagtttaagt ttagcaatta tatgtttatt atctataatt ttatatatca
    11101 aaataaaagg tgaagtttta aaaagaaata atgtattttt actaatatac agtaagatac
    11161 ctatggaatt taatgtaata ttagtatcta tttcaattgc aattttaact atatatgctc
    11221 ttgctgtatc ttatcatatt gatgtaattg cattagtttc tttatcttta ctgatagtat
    11281 caaataatat attttttata aatgtgcaat tgagttattt agaaaataaa atgtgtatat
    11341 ttaagacaag tattatagta agagtgattt tatggttaat aaaaactgta agggaggtca
    11401 gtaacgctat aaagaaagta tctttagcaa ggaaagttat tgttatagtg ataatatctt
    11461 tagctttaat catggtaatt agcactcgaa gtataggtat gggattactg ttttctatat
    11521 gtattttttt aggatttaca atatatataa ctaaaagact ttcatatctt agttacgtaa
    11581 tagaaggtac agaaagaata aaaaagggag aattagatta taagataaaa attactggga
    11641 atgataattt tacgtcttta gctgaaaata taaataatat tggagaaggt cttgatagag
    11701 ctatagaaga gcaagtaaaa agtgaaagaa tgaagtctga ccttataaca aatgtatctc
    11761 atgatttgaa aactccactt acttctataa taaattatgt agatttaatt aaaaaagaag
    11821 aatcaataca accagagtat ataaatgatt atataaatgt acttgagtca aagtcaaaaa
    11881 gactaaaatt attgatagaa gatttatttg aagcgagtag agtaagtagt ggcaatatag
    11941 aattacaaat tagcaagatt gatttagtgc aactattaag acaaagtata ggtgaacttg
    12001 aagaaaaatt atctaaaaat aatttatatt taaaattaaa tgttccaaat gataaggtgt
    12061 atatatgggc agatggaaga agaatgtata gagtatttga aaatctttta tctaatatag
    12121 caaaatattc cttagaagaa actagagtct atattgatgt atatgactat ggtgaaaatg
    12181 taaaggtaac tatgaaaaat atatcttctt atgaattaaa ttttgaccca agtgagatta
    12241 tggaaagatt taaaagagct gatgaatcaa ggaatactga aggaagtgga ttagggcttg
    12301 ctattgcaag agatttagta gctttacaag gtggaaaatt ttatatagaa atagatggag
    12361 atttatttaa agcaaattta gagtttcaaa aatatgaaga agaaataaca aatatggaat
    12421 gagatagtat ttaagtatat atattttttt tactgtcgaa agcttttatt ataatatatt
    12481 agaatattta cagtagagaa tgaagagtaa aaacagagta actttaagtc actctgtttt
    12541 ttatttaaaa tcaataatat aacaacttaa attaaaattg tacacatagt tttaaaataa
    12601 cttatacaat aaaacattat ccaaatatca caagataatt ccaaataaat atatagatat
    12661 cactcctgta ataatagaaa taccaataga tttaaactta tatgcaacaa gaagtacaat
    12721 cataaaagat ataattttag ggttagctat agataaatat agactatctc cattaaaaaa
    12781 tacatcttta gcagttaagg aagtaagtat tgctacaggt ataaatttca tgattttttt
    12841 caaaaaatca ggcatctctt tttttgaaaa aattaacata ggaagcactc ttggtagata
    12901 tgtaacaatg tacattccaa ttataataaa aaatatataa ttattattca taagattcct
    12961 cctttttaca gtaagattct tctataaaga aaccaattaa agatgctaaa attgatgcaa
    13021 ctattattac taaattgctt ttgaataatg catataaaat aacagaaata atgattgatg
    13081 atataaatac tacaatataa atcttatttt ttatttgaga aactattaaa gctataaaca
    13141 tagaagtcaa tatgaagcca gaaatagaac tgtctataga tatactttct cctaaaaatg
    13201 ctccaataaa attagctaaa actgatgaaa ttaagcagaa gaaattaaga taaatagcat
    13261 ttctatcaga ccaatttcca gcagtaaatt tttcataatt aactgcaaaa gtttcatctg
    13321 ttatagtatg acaaaatggc aatataaatt ttggactttt attttttatg tacatgttca
    13381 aattagaact cattagaaaa tgtcttaagt taactataaa tgtagtcaaa acaatagatg
    13441 ttacacttgc accagtagaa atcattgaag ctgcaatgaa ttgagaactt cctccaaata
    13501 ctaaagttac cattagtgct atttgaaaag gtgaaagacc agatttttgt aatagtattc
    13561 ctcctactat acctataggt atataaccta agaatatagg aattgaagca ataaagcttt
    13621 cttttaattg ttgagttctt tccacaataa atcactcctt ttgtattaaa tgtattaaag
    13681 tattagtaaa acagaaaaat agattaattg tataattgca attataatta aaaataaatt
    13741 tggattaatg tataaaatac tatacaacaa ataaataatc ttaaacggtt attaaatttt
    13801 atctatttgt tattatgatg taataataca tattaatctc tataagttaa ttactatctg
    13861 gtgtaatttt attttaacaa caaaaaaaaa tacacacaat taaaatattg tgtgcaatac
    13921 aattattttt tctcttctaa tagtcttttt aatacgctca aaccagcttt aagttcttct
    13981 gtactttctg tagcacatac agctattctt attgcagaaa ttggtttttc ttttccaaca
    14041 gcaaaacgtt cagaagcata gacctgtaca ccttctttaa gtgcttgtat ctcaaatttt
    14101 tctgccatta ttccatcagg tagaatcaac catctaaata tacattcttc atttcccaaa
    14161 agattatatc cagataaata ttgattaata atcttgtttc ttcttcttgc agatttacgg
    14221 cgtgcattaa ttaatttatc ggcttcacct gatgtaatca tgcgataagc aatttctgtt
    14281 aaaaaataag attgagatag attaatgttg tataatgcgc ttgataatgc ctctcgatac
    14341 tgtttaggtg tagagatata tgctagccgt aaactaggtg caataatttt tgataaactc
    14401 gtaatataaa ttgtttgatt gggcagatag gaagccactg gatttaaatg tgtttcatta
    14461 agtaaactat gaatagcatc ctcaataaca attaaattgt atttagatgc aatatttgca
    14521 atcatttttc gtccattttg tgacattata tgtgtagttg gattttgata atctggtatt
    14581 atgtatagac ctttaatatt ttcattttta caagcatata atagtccttc ttcactaatc
    14641 tcattatctt cttgtttaat tggaattagt tgaatcccaa gcattttagc tgctgtcttt
    14701 attccaggat aagttaatgg gtctacacct attctatctc catgttgaaa caaaccagct
    14761 aaaatagcaa caattgcatt ttgtccacca cttgcaggaa gtaagctatc tgatgtagtt
    14821 tcaagcccag ctttataaat taatttggca atagcttctt tttgccattt agcaccatct
    14881 ctttgtccat attggaatat agttttaaaa tctatttcct taattatgtg tttaaacaaa
    14941 gtattcattt cttctagtgt gaaatctgga ttcattgtgc ccatttcaat aaatgtaatt
    15001 ttattattac tggacatcaa gaatagattt gaacgcgtat cataagatac aaaagttcca
    15061 cttcctgtaa ctccacttat tagacctttt ttttcacaaa ttttaaaagc tctagagata
    15121 gtactcacat taacatctaa gaaatctgcc aattcacgtt ggggtggtaa tttagtacct
    15181 ggtaataaaa atccattgtt gatatcttgt tctagttgtt ctgcaagcgc cttatataat
    15241 atttgccctt ttgaaggtct ctttggtttc cagttcatag gataattttc aaaagaatta
    15301 attggcataa gttttcacct cacattatta atttgatttt ttctttatct aaaacaataa
    15361 attctaaaga tacaaataaa aactgatagg aaataattct attagttttt gtcttttaat
    15421 tgatttcaaa tcttcctata atttttatac cttctttatc ataaagaggg attgtttttt
    15481 taaatatatt aaaatttctt tttcttgtat ataaaacagc ttcttctggt gtctttggtt
    15541 gattatcaca atcgtacaaa tttctttttt ttaactaaaa gcaataatac ataaaattat
    15601 tattgctaat gatactctta atgtggtata tagtcatagt gtcacatgtt tatcaatagt
    15661 tttttcatta attattgtaa cgaatagtag gactcataaa atacttagaa atatgttttt
    15721 agatagattt taaatatata atctaactta tatctataaa ttaacactat tattctccaa
    15781 ttccatttct agttttttta taaatttatt aaattcttct ggttgccttt ttccaaacaa
    15841 tactcttaca agagctgaat tgatttttcc aaatatattc ctcttgctct cactttcaat
    15901 aaaaataagt tttgtatttt ctaagtcaat atgtactaat tcatatctag taatagtact
    15961 ttctctttta ttgcttgttt ttatctcata tactctattt ttttcgaaat tagttatttc
    16021 aaccttaaat cttttattat ttttttgctg tacaaatgtt cctactgctt ttttctcatt
    16081 aaaattagga aaatttagtc ttgcagattt tgtaaatata ttaaatatgt cttttgcaga
    16141 atattttaat aaaacttcgg ttttaaatgt attcatatcg ttttcccttc tgtatataaa
    16201 tttaatattt ataagttaat ttttaagtta aaatagtata atatgccata ttaaatcatg
    16261 ttaaaactat ttatatagct aaaatcttac ctatataagt atgtatacta tcatataaaa
    16321 ataaaatctt aaaatagaaa gggttaaaaa cacagtttat tataattttt attgttgagt
    16381 agatacgtac tattatatat gattgagcaa gtttttcaat cataaattta taaagtattt
    16441 tgcattaaaa tttaaacaaa gaattaaatt atttaagatt atgaataact aatacattta
    16501 gtaaattact aaatatacaa aatgtaaata cttatcaaaa tgtataaata cttatgacat
    16561 ctagttactt acagggttta agtagaaaaa aatatcaaag taagtatggc atgatagttg
    16621 cttatatatt tatcaagaaa ggagttatta agcatggagt tgagagataa acttgtaaac
    16681 atcataaata cagaaaataa aaaagatcca ttaacagatg ttcaaatagc aaagttttta
    16741 agtacaacta gagagaacat aactaatttg agaaaagaac ttaatataag taattcaagg
    16801 caaagaagat atccttattt aaaatcagca atttcggcaa tattacaaaa gaataagaat
    16861 atttctatat cagaaattac aagggaattg atgacagaag gctttaatat atctagaaga
    16921 gttgtggagg aacttttacc taaagaaagt ttagaaatgg atgtagtaga ggaatctgaa
    16981 gaagaatcga atgacccatt tgaaactctg ataggtaata aagggagtct tagaaatgct
    17041 gtagagcagg ctaaatctgc tatattgtat ccaccaaaag ggttaccaac gttgattatt
    17101 ggggaaagtg gagttggtaa gagtttattt tctagacata tgtatgagtt tgcaaagcaa
    17161 aaaaagatag taaaagaaag ctccaatttt gttgtattta actgtgctga ttatagtgac
    17221 aatccacaat tattattatc tttgttattt ggatataaaa aaggtgcatt tacaggtgct
    17281 gaatatgata caccaggtct tgtagaagaa gctgatgaag gagttttatt tttggatgaa
    17341 atacatagat tgcctccaaa aggccaagaa atactatttt ctattttaga taggggaaag
    17401 tttaggagat taggtgaaac taatgctgaa agaaaagtaa gtattatgtt gattggggca
    17461 actactgaaa atgtagagac taatttatta ttaacattca gaagaaggat accaatgcta
    17521 ataactttgc caccattaca tgatagatta ctaaaagaga aaatagacct tatatacaat
    17581 atctttcaac aagagtgtaa tagaataaat gcaaaaatat ttgtagataa aaatgtgatt
    17641 gaaatacttg cactaaaaaa gtttagtgga aatataggac aattacaaaa tatgattcag
    17701 gttctttgtg caagagcttt tatgaagttt ataaattcca aagatgaaga tagtgaatcg
    17761 atagtagtag tagatataaa tgaagtttta aagcttaaag attcttttaa agatgctgcc
    17821 tttcaagaga ttgaatatac agagataaga aaatatttga aaaatgctat atttatacca
    17881 tttaatttag aagaatcatc agtaaaagga agtcttttaa gtgatgaata tatattacca
    17941 gaggacatat acaaaacaat tgaaaagaaa tattatgacc taaaatccct agacattgaa
    18001 gatatagata tagagaatat tttatggact tttattttaa atagatttgc tgacttaaag
    18061 tttaatctcg atagtaatga agaaattttt tcaatgagtg atttaaatag ttttgtaagt
    18121 gatagtttag ttaaactggt aaaagaactt atgaatgata tagttgaaaa aaatatgaat
    18181 ttagaagtaa ataaaaatat gttcaaatat ctagcaatac acttagaaga agctataaaa
    18241 agaattaagc taaatcaaaa gataataaat gtaaatttag aaaaaataaa gattgacttt
    18301 tcaaaagaat atgaaattag taaaagtttt gcaataaaaa ttgaggaatc aattggtatt
    18361 aagatttcag atgatgaaat tggatttata acgctctaca taaagtctgc tctaaaaaat
    18421 gaagtgaaaa aagataaagt agggttaata ataatttctc atggacgtat agctacagag
    18481 actgtaaatg tagttaaaga acttttaggt gtaaagtttc ctgtggctat agatatgcct
    18541 ttagatgaaa aaccaataaa tatatacaat aaggcagtag aactgtcaaa aataatagac
    18601 caagggaaag gcatattgtt tttggtggat attggttcac ttacaaacat aggtcaaata
    18661 gtaaataaga gaaccaatat aagtactaaa actattgata gagtagacct cctaatggca
    18721 ctagaagcaa ctagaaaagt ttctatggga gaggaagaac ttgatgaaat atttttttct
    18781 ttgctaaaag ataggatggg atataactat gaattagaaa aacacataga taaatcaaat
    18841 gcaattataa cattatgtct tactggagaa ggaactgcta agtatataag taaaacgttg
    18901 gaagaaaagt atgataacac taaatgttat caaatgagtg cattggatga aaatttattt
    18961 tgtaaaattg aaaatctaaa agagacaaac aatatattag ctattgttgg gacaataaac
    19021 cctaagatac caggtataaa ttttataccc tataataaag aacttttcaa aaatttggat
    19081 atctatctat caaaatcaaa acaacaagat gacagaccag ttaaatcgta tgaaagaatg
    19141 cttgatgagg atttagtgat ttttgaacca gatatatatt ttaaaaaaga tttattagaa
    19201 tatgtatgtt caattcttat aaataaagaa tatgtagaaa aagattactt agattctgtt
    19261 ttacatagag aggaaatgct tccaacatat tctaaaggag ctataggtgt gcctcatgga
    19321 gattcttcta ctgtaaatag tacaaggttt gtttttgtta aactaaagaa tcctattgat
    19381 tggggtgtag gtaatgtaaa tttcatattt atgccagttt ttcaggctaa tgataaagag
    19441 attgttaaaa atgttcttga gatactaaaa gatgcagaat ttatgaacaa tgccaataag
    19501 tgttttgata aagatgcttt tagaaggatt atatttgata aatttaaaca tctgtagcca
    19561 tagcaaaaaa attgagagga gttagagtaa tgaatgtaga tttaatatca ttgggtttag
    19621 aatctaatga ttatgaaagt gttattgaag aattaggaag tataatgtgt aaaaaagaat
    19681 atgtaaaaga aacatacata aatgctgtct tggaaagaga aaggacatta ccaacaggtc
    19741 tagatattgg agaaatgtgt gttgctattc cacatacaga ttcaaagcat gtaaatgagt
    19801 ctaatgtagc tgtagggata ttgaaaaatc ctgtaaagtt taattcaatg atagacccaa
    19861 aagatagact tgatgtagaa cttgtatttt tattagcagt aaagaaccca gattcacaag
    19921 taaaactgtt aaaggattta atgtcagttt ttcaaaatat aaagttatta aaaaatataa
    19981 aaaacgcatc aacaaaagaa gaagtagcta aattattgga ttttatagaa atttaatgta
    20041 ggagaatttt ataaataaaa aattaaaatt taaacaatca aagtaagtat tattttttta
    20101 ataggtttaa tcttatataa aataaaaatt tagagtgaaa aattaggagg aaattattat
    20161 gaaacaattt aatgtattat cagtttgtgg ttcagggaca gttacatcat ctatggtagc
    20221 aggtaaatta aaagaagtac taggtaataa ggggatttct attactacaa cagaagctag
    20281 accaacagaa gcactaaatt tagcacaatc aggaagattt gactttataa catttacgag
    20341 tccacttcaa acaggcgatt atggaatacc aactataaat gcatttgctt gcctaactgg
    20401 tataggggaa gaggcattct ttgaagaagt tttgactgtt attgaagaga tagataagta
    20461 agttttggag acgtgaattt atagtattta aatcaatgat ttagatacta taataagcta
    20521 atattaacta aatatttgtg gaaaaaacat tttatatgtt gtcataaatt aaaaaattat
    20581 aaaaatagtc acttaagtat taaattaagt ttataaatat ttataagtag gagggaaaaa
    20641 gatgttagaa tttatgaaat ctataatcga tacatttggt tctgcaatta tagttccaat
    20701 tataatattc ataatagcaa agatatttaa agtaactact aagaaatcgt ttctttctgc
    20761 tgtgtatgca ggagttgctt tacaaggttt tacactgatt ttaaattcat ttacaccaat
    20821 aataacacca gttatcaata ggatggttga gagtacagga gtaaacctac cagtttttga
    20881 tgttggttgg caagcaacat cattggttgc attttcaaca agtgcaggta tgatatattt
    20941 aggtcttggt atattattac aaacaatttt atttttgata aaatggacag acgtgtttca
    21001 accatcagat ttatggaata actattctta tatggtatgg ggagcaatgg ttataggagt
    21061 aacaggaaat tttcctttag gtatagcatg tatggtttta ttaaacctat atagtttatt
    21121 aatatcagag ttagttgcaa agagatggtc tagttactat agatatccta attgtacaat
    21181 aatagctatg cataatgttg aggcttctgt gtttgcagta tttgcagacc ctatatacaa
    21241 taaattggga ttaaataaaa taaaattaaa tccaaaagaa ttagagaaga aattagggtt
    21301 tttaggtgaa ccaattactt taggtctttt tttaggtatg tttataggta tattaggtaa
    21361 tatgacgaga ataaatacta tggaagcttg gggagaaata atgaaagtag gtatatctac
    21421 aagtgcagtg atggcaattt tccctaaagt agcaagtatg tttgctcaag catttgcacc
    21481 tataacagaa gcagctagaa agattatgca aaaagctgga aaccgtgagt ggtacatagc
    21541 agtaaatgat gctgttggat atggtgaacc tgcaacttta atatcaggtc ttatattaat
    21601 tccaataatg cttgtaatag ctatggtact tcctggtaat aaggtattac cagttgtgga
    21661 tttattggca attccatata tggttcaagg tttagttgcc atacataatg gtaatatacc
    21721 aaaagtatta gtttcaggta taatttggtt tggattagga ttatatgttt gtacatctac
    21781 agcaccatta tttacagata tggctacaaa tataggtgta gctatacctg caggagcaat
    21841 gttaataaca agttttaata tactaggaaa acctttgatg gggttagttt tctttgcatt
    21901 tctaagtgca aatccaattt atataggtct ttcagttgta atttactttg ttctatgggc
    21961 attgtttaga aagaacaaaa caagtattct tgattattta gaaaaacaag cacttaaaaa
    22021 tgtagaagaa gagcctgtag cagtttaagt ttataaaaat gaaataagaa taattaaagg
    22081 agtttataat tgattttata taagtatcag ataatacctg agaaatattt aaatgaaata
    22141 tagacttctt tttatttgat aatatttgta attataatgc attgtatttt tatagttgag
    22201 ttttctgtat aacctgctat ttaaaacata ctcttttata tttatacagt aatgtggttt
    22261 aattttataa aatgtaatct ctactggtaa aagactttgt atataaggca tttgcctgaa
    22321 taaaccattt atcttttgta tggcatagat attgctatat agaagtatat agcatgaaag
    22381 gagatattta tatgggaaat aaaatgagag catcagtatt gtataatgta ggtgatgtca
    22441 gatatgaaat ggtagatata ccagaaataa cagatacgca agtgcttgta aatgtaaaat
    22501 atgttgggat atgtggttca gatttaccta ggtctatggt aagtggtttg agtggaaata
    22561 caaaatatcc attgatttta gggcatgaat tttctggtga agttgttaaa attggagaaa
    22621 aagtaaagca tattaatgta ggagatagag tagctgttgc accattagta ccttgtggaa
    22681 aatgtgatta ttgtaatgaa ggtaattttg gcttgtgtga tgattacaat ataattggaa
    22741 caagagtaaa tggagcgttt gcagaatatg taagagttcc agaagaacat attttaaaac
    22801 taccagatac attggattat gaaactgctg ccggaattga accagcaact atagcttatc
    22861 atgggatttc aaagtctaat attagagtag gagatagtgt agttgtgtta ggatgtggtc
    22921 caataggtca gtttgttata caatgggcaa aggtgtttgg tgcatctaag attatagcag
    22981 ttgatatttt tgatgaaaaa ttagaacttt ctaaacttct aggagcaaac tatatactta
    23041 actcaaaaga agtaaatgta atcaaagaaa taaaaaaaat aacaaatggt ggtgctgatg
    23101 ttgtaataga aactgctggt agccgattta cacaagaaca atctctattt gtagcaaaaa
    23161 agagaggaaa tatagtgttt gtaggtatat cacacacaga attaccactt agtgcagacg
    23221 ctactgagtg tatattacgt ggagaactta ccttgaaagg ttcttggaat tcatatacaa
    23281 gcccttatcc tggaagagca tggactgcaa cattggattt tatggaaaaa ggtgacatta
    23341 tttttaaacc catgatttca gataaaatag gtcttaatga agtgggtgac tttttaagta
    23401 aaatgagcaa gagagaaatt aatttcaata aaatcctagt agaaatataa aaatttaata
    23461 aaaaattcta aaagcaatta aattttataa ctatgacatt aaataaaata aaaataattt
    23521 tcttaaataa aatataggag gaagaaaagt gttagaaaat ttaaaagaag aagtattaaa
    23581 agcaaactta gaattaccta agaaaaagct aattacttat acttggggaa atgtaagtgg
    23641 gatagataga gaaaaaggat tagtggtgat aaagccaagt ggtgtagagt atagtaatat
    23701 gactgtagaa gatatggttg tagttgattt agagggaaat atagttgatg gcaaattaag
    23761 accttcttca gataccccga ctcatctagt tttatataaa gagtttgaag aattgggggg
    23821 aatagtacat acacattcaa gttgggcaac aatttgggct caaataggaa aatctatacc
    23881 atcttgtggt actactcatg cagattattt ttatggaagt ataccatgta ccagaaaaat
    23941 gactagagaa gaaatttgta atgaatacga aaaggaaact ggaaatgtaa tcattgaaga
    24001 atttatggga agaaatccaa ttcattgtcc aggaataatt gtaaatgacc atggaccttt
    24061 tacatgggga aaagatgcaa atgaggctgt gcacaatgca gtagttttag aagaagttgc
    24121 aaagatggct tattatacag agttaatgag tcctgataat attatggata aggttcttat
    24181 gaataaacat ttttcaagaa aacatggaaa aaatgcatac tatggtcaaa aatgattatc
    24241 tggaggtgaa ttatgctaga ctctttaaaa ggtaaagttg tggaaatagc aattaaagca
    24301 gagcattatg gtttatgtaa agaaaagact ggtaatttta gtattagaga taaggatact
    24361 ggatatatac ttataacccc atcaggtata ggaagagaag aattaaaagt tgaacatatt
    24421 tgtgtattgg atttggatgg aaatgtaata gaagttgaga aaggtattaa gccaagtagt
    24481 gaggtattga tgcatattga aatatacaaa acaagagaaa atataaatgc catatcacat
    24541 actcattctt tatatgcaac agcattttca gtagcaaaga aaccaattat accaattgta
    24601 tatgaatcag ttaattatgg cggttatgta gatattgctt catacgaaag accaggaact
    24661 ttaaaattag ctaagagtgt ttcaaaatta ctgatagata cagatgcatg cttattagaa
    24721 agacatgggt tgacaactgt aggtaatgat ttagacgaag cattattaaa atcaagatat
    24781 gttgaagagg tagcagaaat atactatagg tctttagtat taaatcaatt taaagaactt
    24841 gaagtagtag atgttgatga gtttaaatct tggaaatacc cagaagaaat aaagttttaa
    24901 tctaaataat tttcattgaa gtggttaatt aaaatatatg tattatttat atcaattact
    24961 atgttttatt atgttaatta ccgtattttg caaatatatt tattacattt tttcaaaaaa
    25021 atatagaatc cgaaagccat ataaaattga cttgggattc tatacttttt atttatgcat
    25081 ttttctttat ctgattttct ggagaattta tatgttcaaa atatttttta gtttcagata
    25141 ctattatagg tgataatgca agtaaagcta taaggttagg tattgccata agcccattta
    25201 caatatcagc aattatccaa acaacttcta actttaagaa agcacctaat aacaccatag
    25261 ccacaaatac agttctatac ccattcatgc cttttacacc aaacagaaat tcaaaacatc
    25321 tttctccata ataactccat cccaaaattg tagtaaatgc aaataatgtt aaactaactg
    25381 taagtaatat tggtccaaat gtaggaattg ctccgttaaa agctgcttgt gtcataacag
    25441 ctccatttag gtctgaacac cacactcctg aaataacaag agagaatcca gtaagagtac
    25501 agattataat agtatctata aaagtacctg tcatagatat aagaccttgt tccgctggcc
    25561 attcagtttt agcagcagct gcagctattg gagcactacc tagaccagac tcatttgaaa
    25621 atacaccacg agcgatacca tttctaattg ccagtgacat agttgctcct aaaaaaccac
    25681 cagtagcagc agtaggtgta aatgcacttt taattattaa cataaaagtt tcagggattt
    25741 tattccaaaa agatattaaa atgattccac agattacaac atatataact gccataaaag
    25801 gtactatttt agtagctaca ttagatatac ttttaagtcc tccaaataca actaaagcta
    25861 caaatactgc taatacaacc cctgtaattc tagggtctat cccgaaacta ttatttattg
    25921 catctgttat tgaatttact tgagtaaaag taccgattcc taaaagtgca actagtatac
    25981 cacttatagc gaaaaatatt gcaagaggtc tccatttttc tcccatacca tttactatgt
    26041 aatacattgg cccaccagac acttgaccat ttttatcttt agttctgtat tttattgcaa
    26101 gaacaccttc tgaatactta gtagccattc caaagaatgc tgctatccac atccaaaaaa
    26161 gtgcaccagg tccaccagct ttgatagctg tagcaacacc aacaatatta ccagtaccaa
    26221 ctgtagcagc tagagcagta cataaggctg caaaacttga aacatcacca ctacctttgt
    26281 tttcagcaga gaatattaac ttaagagctc ttggcagttt agttatttga acaactccaa
    26341 gcttaacagt aagtagtata ccagtaccaa ctaatagtac cagtaggggt ggtccccaaa
    26401 taaaagcatc aactttgttt aaaatatcaa ttagattcat aaaaacactc cctcattttt
    26461 tttgctttac ataatcaata gaatttttaa tgaagtaatt gacaggaaat aagtataatt
    26521 tattaataat ttctagtgta ataaaaaaag ctaaatctaa aaaagattca gctctggatg
    26581 tacactaatt agcaaccaaa ataaaagtat tagtaaatac tgtttttggt agataatccc
    26641 tgtccttttg cctgagagat tgagcataaa agccttgccc cttcggcgcc cataatgggt
    26701 ctctccagag ttccgtaaat tttacagtcc tacttttaaa agtacctgag agtgttactc
    26761 cttcggtgtg ctatatagca actcttctat aaaatcttaa ccggatattg atttgtcata
    26821 gcttattgta ttaataatat taatataagt ttttatgttt tgtcaactaa aaaattaaaa
    26881 aacaaagaaa aaatataaaa atgtaagcct atttacgaat tataaactta gaaagatgag
    26941 taatatacgt gaaaatgaat tttgtaatac acatacaaat gaagtgtaaa ataagtttta
    27001 aataaaaaaa taataaaatc ttaaataatt aattagatat gattaaatta tggtaatatt
    27061 atagttacga tattaagaaa agttctattt agttagattt taagcgtaat atgagattat
    27121 gtatagagaa aggatgtatt tttagatggg aaaaatatta gtgctagcag aaaaaccttc
    27181 tgttggaaga gatatagcaa aagttttagg gtcaaaaaat gaaaaaaatg gttatataga
    27241 ggggccaaag tatgtgataa cttgggcact aggtcatttg gttacacttg ctgaccccga
    27301 aagttatgga gaaagatata aatcatggag cttagaagac ctgccaatac ttcccaagca
    27361 ccttaagact gttgtaataa aaaagagtgg aaagcagttt aatacagtta aatcgcaaat
    27421 gaatagaaat gatatagatg aaatagttat agcaacagat gcaggtcgtg aaggagaact
    27481 tgtagcaagg tggataatag aaaaatcaca ggtaaaaaaa cctataaaac gtctttggat
    27541 ttcttcaagt acagacaaag caataaaaga cggatttgca aaattaaaaa gtggaaaaga
    27601 atatgaaaat ttgtattact cagcaatagc acgagcagaa gctgattgga ttattggaat
    27661 aaatgcaacc agagctttga caacaaagta caatgcacag ctttcttgtg gaagagtaca
    27721 aactcctact cttgcaatgt tgcttaaaag agaagaagaa ataagaaatt tcaaacctaa
    27781 agagtattat ggattagaat taattgctac aaagggaaat tcagatataa aatttatttg
    27841 gaatgataaa aacaataatt cttcaacttt tagtaaagaa aagatagaat ccacattaaa
    27901 aaaagtaaaa ggtgtagaca taaaaatatc tgaagtaaac aagtcatata agaaaaaata
    27961 ttcacctgca ctctatgatt taacagaatt acaaagagat gcaaataaaa tgtttggatt
    28021 ttcagcaaaa gaaactttat ctataatgca aaaactgtat gaacatcata aagtacttac
    28081 ttatcctaga actgattcta gatatttaac atcagatatt gttggaacat taaaagatag
    28141 aataaaagct gtaaatatta gtgattattc aaagatttgt aatagattat taaaaagcaa
    28201 tattatagct aataaatcgt ttgttgacaa ttcaaaggta acagaccacc atgcaataat
    28261 accaacagaa cagaaggtat tcatgagtga ttttagtgat aaagagcgaa aaatatataa
    28321 tcttgtagta aaaagatttt tagcagtact atctcctcct tttgaatatg agcagacaac
    28381 tataagtgca actgtagaag gagaaatatt taaggctaag ggaaacaaag taataaattt
    28441 aggttggaaa gaaagttata aagatattga agatgaagat gagtatgaaa gtataccaaa
    28501 tgttttagaa aaagatgtac ttaaggtcaa gggattaaag ataacttcag gaaaaacaaa
    28561 tccaccacca tacttaaatg agggaacatt attgactgct atggaaaaga atggtctagg
    28621 gacagttgct acaagagcag atataataga aaaattattc aattcatttt taatggaaaa
    28681 gaaaaataaa gatatatata taacatcaaa aggaagacaa ttattagatt tagttccagt
    28741 agacttaaag acaccagaac ttacagcttc atgggaaaag aaattattgg atatttcaaa
    28801 gggaaaactt agtaaaaata cattcataaa tgacatgaaa gattattcaa aagatattgt
    28861 actagaagta aaaaatagtg ataataaatt taaacatgat aacttaacta aaaatagatg
    28921 tccagaatgt ggaaaattta tgttagaggt aaatggtaaa aaaggtaaga tgttgatttg
    28981 tgaagataga gaatgtaata ctagaaaaac tatatcacaa gtaaccaatt caagatgtcc
    29041 taactgccat aaaaaattag aacttcgtgg agaaggtgag gggagaatct tcacttgtag
    29101 ttgcggatac agagaaaagt tgtcttcttt caacaagaga aagcaagaag aaaaacagaa
    29161 ggcatcaaaa aaagatgtta atcaatattt aaaaaatcaa aataaaaatc aagagacatt
    29221 taataatcca tttgcagaag cattagctaa gctaaaaaac aaataatttt aaagttatta
    29281 ttatacacaa ttataacttt aactgtatat tagttatatg tactgatata atatagtgta
    29341 tagtttaggc atgaaagtag ggctgttaaa tggatttaag tggcatattt aaatactatt
    29401 gtaaagagtg tgaaaataca tggaataatt cgagtgttga attatttgag aatatagaaa
    29461 cgtatagtaa agattcacaa aaaaagaggg aaaaagaatt agataaattg ctaaatacaa
    29521 tatcagttca tttagagagg tatccaagtg atgctgtatt gagaaaaatg tgggtaaaaa
    29581 agggcgaggt tttcttacaa aagacattgg aaaaagaaaa tatttttaag ttagaaaaaa
    29641 tggatgtaga ggatagaaaa aaatttttag atataacaaa acagtttatt agagatgcta
    29701 gaaaatttga tgatgattta cctataggtg atattatgca agctatgagg aatgtatgga
    29761 tttcaaatgc attacaatta ttatttggta aagaagtata ttattcaaaa gctaactttg
    29821 catatagtat gttatatcca tatactgata attatttaga caatacaaat atagataaaa
    29881 atgataagat tttatttaat aactggttag aaaaaaggct cctgggagaa cacattaaat
    29941 ctaaggatta tcatgaaagt aaagtatctc aaatgataga ttatattgaa agtgtatacc
    30001 ctagagaaaa gtttacagaa gtttatgaat cgttattatt aatatttaaa agtcaagtaa
    30061 atagtttaaa acaacatggt aaggaaaatt atttgtgtaa agaagattta ttatccattt
    30121 ctattgaaaa aggaggttca tccgttttag tagatggata tttaataagt ggattgatga
    30181 caaaggaaga aatagagttt tgtataggat atggattttt attacaaata tctgatgatt
    30241 tacaagatat aaaagaagat ttaaaataca accataaaac tattattaca gagatgtcaa
    30301 aagagggtac tttagataaa gttgtaaata aactaataaa ttttactatt gagttaatag
    30361 atagttttaa aattaataat aaaaataaat ctgtaataac tatgataaag aatgattgct
    30421 taatgttaat tttattttct gtagtttata atgctgaatt tttttctgta ggatatataa
    30481 aagaagtaga gaaatttatt ccatatacaa tagattattc attagagatt gaagaaaaaa
    30541 taaaagaaaa atttaaaaat atagatgttt taaataatga aaatgaatat aaagaaatga
    30601 ttgatattat ttgtgcagag tagaaaaaaa gggagtgtct tagaatagac tttttagttt
    30661 atgaggcact ctttttgtat aaaatcaaat gtattttcat cattttaaca attaaaaaga
    30721 gcttatccct atttgagaaa gcccttttta atttaaataa aaatgtcaag gttaaactat
    30781 ttaaatgcag taataatccc attatcatgt aagtttttta tgattgtagc tgcgataggg
    30841 aatccaaata atccagctat tccaaatagt tgagccccaa caaacataca cattaatgtt
    30901 attaaaggat ataggccaat ttggtgtcct actacctttg gttctaatat ttgtctaaca
    30961 acggttataa ttatgtatag tattaaaagt cctattgcta aattaatatt tccattaatg
    31021 agtgagataa tcatccaagg tgtcaaaata cttcctgtac ccaagactgg caaaatatca
    31081 acaagtgcta tgatgacaga tatagtaaat gcattttcaa cctttaggat agtaagtcca
    31141 ataagtagtt ctatgaatgt cactgatagt agtattgcat aggcttttag tagttttaaa
    31201 agagcattaa tactgctatt ttttatattt attatcataa attgactttt ttctggaaat
    31261 tgttttaaga taaagtttac tattttttga tagtcaaaag taaagaaaaa agaagcaatt
    31321 aaagtaataa taagttttaa cataaatgca ggtgttttac ttgcaatact agcaatagca
    31381 ttgagtgcat ttgttgaaat agaagctact aaagacatca tagattgact tatattatcc
    31441 caaccaagta ttaatgctag gttaacatta ggtgtactag catctatctt tgaaaataaa
    31501 gtatttagcg ttggttgtat acttgattta tatatttcag gaagtctaaa aaatacatca
    31561 cttacacttg caaatatctt cacaccaaag ctaactaata aaaatataca caacccataa
    31621 aaaattaaaa gtattactat agaaataagt gtacgattta aatttgtatt atttgtaata
    31681 agtcttataa taggtcttaa gatggatgca at
//
LOCUS       DBGJFF010000030        31671 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.030, whole genome shotgun sequence.
ACCESSION   DBGJFF010000030 DBGJFF010000000
VERSION     DBGJFF010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 31671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31671
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.030"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(361..768)
                     /locus_tag="ACOIAF_001499"
     CDS             complement(361..768)
                     /locus_tag="ACOIAF_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HGZ2668513.1"
                     /translation="MSKVAKATFYLMIVTIISKILGMGRELVLSSIYGTGLYTESYLT
                     AMNIPNIIFAAIGTAIVTTFIPMYQDISSKQGEKQALKFLNNVLNIIVGICIVVAILG
                     VIFSKQLVSIFAIGFEGERFLLTVKFTKILIKK"
     gene            complement(1242..2126)
                     /locus_tag="ACOIAF_001500"
     CDS             complement(1242..2126)
                     /locus_tag="ACOIAF_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HGZ2668514.1"
                     /translation="MDTKKENNYENYICVGSSNVSNAMKMYLKEIEEYKMLSAGEEVE
                     LAKEIINSSSVAKEKFINSNYRLVVSIAKRYKRDSIDMLDLIQAGNIGLIKAVEKYDY
                     KKGYKFSTYATWWIKQSITRYIDDCENTIRIPIHLHQRINFVKKKKQELLNVLLREPT
                     IDEIADACGLEVDKVLELLRRDKNVVSLDTPLKEDEDSSLVEFIPSDADFKDVVIHEV
                     EQHNLKEKIEELLTGLGEQEQQVLRMRFGIDDDDPKTLEQIGKVFGVTRERIRQIEAK
                     AIRKLRHPSKLKQLKHFY"
     gene            complement(2417..2893)
                     /locus_tag="ACOIAF_001501"
     CDS             complement(2417..2893)
                     /locus_tag="ACOIAF_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HGZ2668515.1"
                     /translation="MSKELVKDRVIKYLIEDLLVPQDMIDTNVELAEFEEGAEGILDI
                     VVNVKDEEDYYAPVMIVQCLDEDVELEGEVLQKQIEFLEDVDNITMSGRLVLTNGDAM
                     MYADWRGEEYDTEAALPTYDIMVKEFHEMEQQAKDLEEHHHHDENCGCGCNHHHEN"
     gene            3160..4335
                     /locus_tag="ACOIAF_001502"
     CDS             3160..4335
                     /locus_tag="ACOIAF_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HGZ2668516.1"
                     /translation="MKFKKQISSIVVSTMLVLGSMNLSYADGTNVVTLGANLSESQKQ
                     QMLNYFGVKKDQVLVLDVTNAEERQYLQGVATEGQLGKVTISCSYVEPTKKGNGINVK
                     TANLTWVTSSMIASTLTTAGLEDANVIAAAPYPVSGTGALTGIMKAFEDASGKKLDET
                     KKEIASEELVVTGDLGDDIGQEKATGVMNDIKTEIVKNGTKDTNQIANTINNVVNNYN
                     ITITPEQKEQIKGVMKKIADQNYDYNSMKNTLDNVSDNVNEQLKKLGESVKGSGILDT
                     IGGWFSGIGDWFSGLFSGGDKKDLGILNNTNDESLGSDAVINSTEGVQINPQNNTNSN
                     NDSDTNSSKNDTSSNNENNDSSQTNEDNTTNNSENQSNNNDSVGFFEKIKNWLSGLF"
     gene            complement(4416..5219)
                     /gene="proC"
                     /locus_tag="ACOIAF_001503"
     CDS             complement(4416..5219)
                     /gene="proC"
                     /locus_tag="ACOIAF_001503"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HGZ2668517.1"
                     /translation="MKKIGFIGAGNMASAMIGGIVNSKLVEPNMVIASAYSQGTLDRI
                     DANFGINTTKDSKEVTRTSDIVIVAVKPDIYDDILEEIKDFIDDNKIIVTIAAGKSIK
                     DIESIIGEDKKIVRTMPNTPALVNEAMSSLSINKNINKEDLEAVTEVFNSFGNTEVVP
                     EYLIEAVIGASGSAPAYVFLFIEAIADAAVIAGMPRPQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPGGTTIEAVKVLEEEGFRASVIKAICACIEKSKKMSE"
     gene            complement(5259..5849)
                     /locus_tag="ACOIAF_001504"
     CDS             complement(5259..5849)
                     /locus_tag="ACOIAF_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HGZ2668518.1"
                     /translation="MVLNKDIGAKIKQLRTQKQMTLKDMSEKTNLSIGFLSQLERGLT
                     SVATDSLGKIASVLDVELTYFFMKPKEHKRAVLRSYEKEVFDVENSTFIHYHLSSSLK
                     EKTMLPRLIEILPSKSSEEICCYVHEGEEFVYVLEGTLTVFLGDEQIEMYPGDTIHYN
                     SEKNNHNWVNYTNKVVKILVVSIPNPFEKSDAVKEA"
     gene            complement(6728..7336)
                     /locus_tag="ACOIAF_001505"
     CDS             complement(6728..7336)
                     /locus_tag="ACOIAF_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HGZ2668519.1"
                     /translation="MEKDFFRKNGIDLAKSILGKYLIRKYENKVIVTKIIETEAYMGV
                     NDKGAHVYGNKKTDRTKPLYLDGGHIYVYLIYGMYNCLNLSANIENVPECVLIRGVEP
                     ITSLDEISMNRYNKAYTELSKYQVKNITNGPGKLCKALKIDRSLNSKSIMGEELYISD
                     FYYDDKGKKVFSKDELDIKTSKRINIDYAEEAKDFLWRFYIE"
     gene            complement(7445..10183)
                     /locus_tag="ACOIAF_001506"
     CDS             complement(7445..10183)
                     /locus_tag="ACOIAF_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGZ2668520.1"
                     /translation="MESKGTLSKNKVYILANGLFLVIALLNINLYTVSINMYRIILLW
                     IFGYSIVYISSFKTDEFINILKVGCLVLVFVSILGVVNLKIQKFQIVLLNLSIINMST
                     FIYLTISKVKKLRISPILIFYSAFVILDYFIRNIFSDINWNMIIILINICISMFNLLY
                     LLNKLDSNIEHYKLIKDLTYFVFFTTIICFVAISKINIVDSDRLIGVIYLLLCNHTYY
                     VYVYGLNNRVVYPYYELDSINKKLSKKSEQLGKINLAIEKDMIIQRTLKNYIDQRKEL
                     LRQALDTIPNVWIVTDYDFNISYTNNKFKDEFSKDMNNYYKILTFIKEDEKVAEKLKK
                     FDNDISIIDKLVELNDKIYLLSLSNNKDESNYLISLNDITNEIKIDEEIRNINKDYEN
                     IILNIPSPILVRSAEGGIKKNKVISINKKYEKSFKCVSSDLVDMTLEQYFETFNIDFF
                     DNREFKRLNLTQEKKAEIVSYNDTANCIVNFVMSDSEGEEHVEEVRVGDYWSDNKLFK
                     LLTFRDITKEINILRTVNEQKIIYEKLLDVIPEAIFLEDLSTSRVLYTNRAFRELFGI
                     SSDVLGVTTQKYRNILVKKYINNLNIGEREKSIHIVNENNHIKEVKMISRTLYFGQKR
                     SRVRIIKDLSVQRESERLKKALIKQRQYDQMKMEFYANISHELKTPLNNIYSSVQLIE
                     NLYKKGKIIDFQDILKEHIKITKQNMFRLLRLIDNIINISQVKSDIYKIKAVNFDIID
                     ITERIVTSISSYAKSKGIDLIFDTDEETVMVGLDPESIERIILNILSNAIKFTLEGGE
                     ILVGIYKKDETVEIIIKDTGVGIDKEKLNDIFNRFKQIENSGISNEFGSGIGLCLTKS
                     LIEIQNGKIYIDSKVGEGTSVKIIFPIKEVVEEVYDNSNYNDNIEKFEIEFFDIYK"
     gene            complement(10331..11122)
                     /locus_tag="ACOIAF_001507"
     CDS             complement(10331..11122)
                     /locus_tag="ACOIAF_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HGZ2668521.1"
                     /translation="MKLKKLLSVALVSAIAISAVGCSNKEDKKILVGASSNPHAKILE
                     VAKPLLKEKGYDLEVKIFDDYVLPNTALDEGSLDANFFQHIPFLEETVKEKGYKLTYT
                     SKVHIEPMGFYSEKVKSLDELKDGAVIAVPNDATNGARALKLLAKNKLIEVKDGELIT
                     KKDITKNPKNIVIKEMNAEQLPTVLKDVDGAVINSNYALTANLNPTKDAIVIESSDSP
                     YVNIIACRENNKDSDKIKALSEAMNSKEVKKFIQDEYKGSIVPAF"
     gene            complement(11158..11814)
                     /locus_tag="ACOIAF_001508"
     CDS             complement(11158..11814)
                     /locus_tag="ACOIAF_001508"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HGZ2668522.1"
                     /translation="MNSLIDFLTTLFPNALLQTLYMVIVPTIVATILGFILAIILVVT
                     KPDGLKPNSTINSALGFIVNIFRSFPFMILIVAMIPITRLIVGTSIGETAAIVPITIG
                     AAPFIARIIESSLNEVDKGLIEAAKSFGATKRQIVFKVMIKEAMPSIVSGITLSIISI
                     LGYTAMAGAVGAGGLGNIALIYGYQRFDTAVMVYTVIALIILVQIIQGVGNLAYKKLK
                     "
     gene            complement(11807..12772)
                     /locus_tag="ACOIAF_001509"
     CDS             complement(11807..12772)
                     /locus_tag="ACOIAF_001509"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HGZ2668523.1"
                     /translation="MISIKNVNKYYGKIQVLKDVSIEIESGEIFGIIGHSGAGKSTLL
                     RCINGLEEYQEGSVLVSDKEVKSLNEKQMRDLRKELGMIFQHFSLLERKTVFDNVALP
                     LECFGYSKAEIKKRVLELLEVVGISEKKNDKPRNLSGGQKQRVAIARALALNPQVLLC
                     DEATSALDPNTTKSILSLLEDINKKLGITIIVVTHQMEVIKQICGRVAIMENGEVLEV
                     GDTEEIFLRNTKGLRKLIGEESIILPKGTNIKILFPKDISNEAIITTMARELNIDVSI
                     IFGKLEQFKDDILGSLIINISDKSGEQVKQYLTSKGIRWEEMINE"
     regulatory      complement(12878..12984)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(13274..14218)
                     /locus_tag="ACOIAF_001510"
     CDS             complement(13274..14218)
                     /locus_tag="ACOIAF_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGZ2668524.1"
                     /translation="MSRAERLIELMITINAKRSFTAGELAEEFSVSKRTILRDLQVLE
                     SIGFPLYSKVGAAGGYHVLKERILPPIAFSESEVKSIFFAYQSLEYYNDLPFEQETIS
                     VLKKFLNCIPNDIQYNIENIRRKFVFWTPDRHCSTPLLKELFNIVMNEFTIKIEYSSK
                     QKNSVRTIVPIGLYAMNGLWYCPAYCIKSESIKEFRVDRIVKILSIENLSSKKYKVLS
                     SIHDYLKNMEVGTDYHIKINLTDEGVKRCETEFLLARGLKILSKGGYIDMYIPKSTLN
                     WVAEYFLTFGKNATIIEPIELKHLIKSKVLELYNHHCI"
     gene            14329..14796
                     /locus_tag="ACOIAF_001511"
     CDS             14329..14796
                     /locus_tag="ACOIAF_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6194 family protein"
                     /protein_id="HGZ2668525.1"
                     /translation="MENDTIKADDILNYCLSNLDDVVLMDSWGERAIYYNPNGVLKRG
                     VYVLTIKEKDSNNDKGSLVSRPNVYRVNIGLKKETFIEMFGYIPKRPGVGQIVDMDFD
                     FTKLDTIMPHPIYSWMGWICVLSPTEKTFENFKTLIGESYNFAKQKFKKRKNK"
     gene            complement(14966..15565)
                     /locus_tag="ACOIAF_001512"
     CDS             complement(14966..15565)
                     /locus_tag="ACOIAF_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668526.1"
                     /translation="MKLKRTLFVGLALVLAIGITACTKNNEKPSENTGKNPNESVNNN
                     EGYMNYYSTSYNDYIAGLNRYSIYDTPESINNQFKDKEYPGNEKYLSDVKAAYKDSRD
                     KIQSFVTSLKKDGKTEDTELKKMNDDLIAEGERLVKDIDKRIEKLDKVSDEDMKKGQN
                     DFIKLVHGTEDVGNDIGSGFRKMIKDMNDRLGITGNNKK"
     gene            15811..16407
                     /locus_tag="ACOIAF_001513"
     CDS             15811..16407
                     /locus_tag="ACOIAF_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3793 family protein"
                     /protein_id="HGZ2668527.1"
                     /translation="MSCCNSQFCCKNKIESSYIKWLLEVLGPVFLGSKPAEIINISFT
                     DVNREEKINDIYKYLYKCKKIDFIVIDKEKKGLKILFVNKKALSEKLKCKKTVNFLKF
                     LGYKQNTNVNAYLEHLVDKLKSDVFPDEIGIFLGYPLKDVIGFMGYSNYEVSMIKYWK
                     VYGDTKQSEDTYSKFLLHRKKMRKLLDYISVDKIVSCF"
     gene            complement(16505..17464)
                     /locus_tag="ACOIAF_001514"
     CDS             complement(16505..17464)
                     /locus_tag="ACOIAF_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HGZ2668528.1"
                     /translation="MTSLIMVLVGTVGCSTTNDKKDEPAKKEASLPKEINLTYVKSPL
                     NVPSILEKKDDLFGKEFKKDNIKVNFHELTTGPEQTQALAAGELDFLHALGGTSAIIA
                     ASNGVGLKITNIYSRSPKGYMLITNNDNIKSIKDLKGKKIVGPKGTILHQLLIAALAK
                     EGYTIDDVEYVNMDIPSAASALESKSADVAMLAGPVALKTINSGAKMIVNGEGLVSGI
                     IVTAVSDDFAEKYPELVKRFMKVHEETLKYMNENKDEVMDVVSKEVGLSLDETKEMYS
                     WYDFSSKITDKDIKELEDTQEFLMSNGMQQKKINIKDMLYNQN"
     gene            complement(17498..18229)
                     /locus_tag="ACOIAF_001515"
     CDS             complement(17498..18229)
                     /locus_tag="ACOIAF_001515"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2668529.1"
                     /translation="MVRSGFLLENIYKKYLVDNKEHLVLDNISLNISSEEITVILGES
                     GCGKTTLLRILAGLENATSGNIYFFNNDKKCTPKVGMVFQESRLMPWLNVSENILLHT
                     EKDNRNKVDLDKYLKMMKLEKFKNSYPNELSGGMAHRVSIARALSFNPDILLMDEPFA
                     ALDYFTRRKMQKEVVNIHKNTKKGVVFVTHNIEEAMEIAKKIIVFSKNKRIKQFSVED
                     EYNRDLTKNYYINLKKEILRELGEF"
     gene            complement(18222..18992)
                     /locus_tag="ACOIAF_001516"
     CDS             complement(18222..18992)
                     /locus_tag="ACOIAF_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2668530.1"
                     /translation="MFEKIHKRLKGYYIFLIILILWKIVCVLGIWSSYILPPPEIVLD
                     TFIKMIYDGSIFLNVYASVRRILIGFLISSLMAIPLGVLFGVNKKMYEYFKPLFEFMR
                     STPPLALVPMLILWFGIGEESKIIIIILASFFPIFLNTLKGIKNCDNKLIEVGKSFNL
                     SKKRIFYKIIIPNSMLDIVVGLSLGLGYSFRAIIGAELIAASSGLGYLISDGKDMSRT
                     DVVLVGIFVIGFLGILSDYLFSKIITKFSKGKQVDLNG"
     gene            complement(19077..19787)
                     /locus_tag="ACOIAF_001517"
     CDS             complement(19077..19787)
                     /locus_tag="ACOIAF_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HGZ2668531.1"
                     /translation="MFKFDVTNELMCIARGSGKFFAKKGAMVAFKGNFNFEKLLLGPS
                     NGGGLGRALLGHVQRSLTGEQMPIMVVEGEGEIYLAQNAYHVDVISIDPGDSIYVESE
                     NLLAFSEQLNYSVKLIGSGVISQKGLFTTHLVNKTGQNQDVAIITDGNPLILEGPCCV
                     DPDALVAWTGREPYPKVSKLSWKTFIGQTSGESYHMEFVEPGQIVIIQPSERLSGLKT
                     DSTPSDRMSSGGLRLGID"
     gene            complement(19967..20119)
                     /locus_tag="ACOIAF_001518"
     CDS             complement(19967..20119)
                     /locus_tag="ACOIAF_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HGZ2668532.1"
                     /translation="MATFIIAAIVVVLMALAVMYMVKNSKKNGSSCGCGCSGCSSSKS
                     CHSAKR"
     gene            complement(20145..22301)
                     /gene="feoB"
                     /locus_tag="ACOIAF_001519"
     CDS             complement(20145..22301)
                     /gene="feoB"
                     /locus_tag="ACOIAF_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HGZ2668533.1"
                     /translation="MSIKIGLIGNPNCGKTTMFNGLTGSSQYVGNWPGVTVEKKGGKL
                     KGNKDVEIVDLPGIYSLSPYTLEEVVTRNFMLDDKPDAVINIVDASNIERNLYLTTQV
                     LELGIPTVIALNMMDIVNKNGDKINIKELSEVIGCPVVEVTAVKGQGIMEAAEKAVEL
                     ASSNNKLNFKLPFVDESKDAIEKIEKIIEEKTPYIDVETRWLAIKLFERDENVIQKLN
                     ISKTILNSIEEITRNCEDELDDDSESIITANRYEFISSIISSIIKKNRKGKETVSDKI
                     DKIVTNRILALPIFALIMWGVYYIAVSSLGTIATDWTNDVLFGEIIQGNVSNFLASLN
                     VAEWLQGLVVDGLIGGVGAVLGFVPQIMLLFLLLSILEDCGYMSRVAFIMDRIFRKFG
                     LSGKSFIPMLISSGCGVPGVMSTRTIENDRDRKMTIMLTTFIPCGAKIPIIALFAGAL
                     FGGASWVAPSMYFLGIAMIIICGIILKKTSLFAGEPSPFVMELPQYHIPSAKGVLIHM
                     WDRGKAFIIKAGTIIFVACGVIWFLQSFNWSLQMVDAGDSILASLGNIVAPIFAPLGF
                     GNWQSSVATVTGLVAKENVVGTFGVLFGISDATEQDPTLLASVASMFTVASAFAFMAF
                     NMLCAPCFAAIGAIKREMGSWKWTWITLGFQTLTAYIIALLINQVGSLVLGTGGSIAG
                     AIISIFIAVAVVFVVLTYSNKNMKKEKMGKLSYMKN"
     gene            complement(22401..22622)
                     /locus_tag="ACOIAF_001520"
     CDS             complement(22401..22622)
                     /locus_tag="ACOIAF_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HGZ2668534.1"
                     /translation="MNRLKDIKCGETVKVKKLEGEGATRRRIMDMGITRGVNIFIRKV
                     APLGDPIEVTVRDYELSIRKSDAEKIIVE"
     gene            complement(22635..22847)
                     /locus_tag="ACOIAF_001521"
     CDS             complement(22635..22847)
                     /locus_tag="ACOIAF_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HGZ2668535.1"
                     /translation="MMPLILSNTGEEVVIKKISGSDKTRTFLNSIGFVVGTNVKIVSK
                     IDGNLIIDVKDTRVALDKKMASRIII"
     gene            complement(23168..26065)
                     /locus_tag="ACOIAF_001522"
     CDS             complement(23168..26065)
                     /locus_tag="ACOIAF_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HGZ2668536.1"
                     /translation="MKAFKKHFLILSLIFLLIISCFNNILCFADESENSKKIRVGYCD
                     DYGIISSSKEHSYTGYGYDYLREISKYTRWEYEFVKGSWEECLDRLEKGEIDLLGPLQ
                     KNDERNKLFNFPKLSSGYEYSALYTKDSNNNMFYEDISSFKGMRVAVLRGNFHNTAFE
                     KYREENNFSVEYIYCNSIDELIESVNEKKADAFVCGSIINAKGMKIVSKFSVEPFYFA
                     TAKDKPNLVKELDYALKELKINDMYYELELYKKYFKREVNNSIAFTRQEMNFIKANPK
                     LTMVYDSEWSPVEYYDKESNSFKGISSDLMSIISKKCGIKFEYIKTKNYNESLDYIKS
                     GKATMLCGSINENDKAKRFNMKLTNPYINIPMIMVGKLDTNLNNDLNIALTSSYKSID
                     SYIEKSFENAKTTSYKSVESCLNAINEGKANLMILSSYQFDELLREGKSENLSVISVL
                     DTSYGMRIGVSNKTDPILVSILNKSIDKITEEELSDCIYSNTIDKPYKVPFGVIFKEY
                     SIQIISFVCILFLIAIKYIIYNKKKKEDYLRKIAYTDPLTGADSIDKFKINSNKLFDK
                     NNPEEYALFYIDVDKFKYINDMFGYDMGNDTLIHISNTIASELKEDEIFARVSADHFV
                     LLIKYKTDDDIKTRLNNIYNKVQILSNPKINYYKLILDCGIYKISKSDNDINTIMDRA
                     NTARKTIKGGHKNSFAFYDKEMHKKILKEKEIENSMVDALNNGEFIVYFQPKYSLSDY
                     QIIGAEALVRWDNPQKGLIPPIEFIPVFERNGFIVNIDFYVFEEVCKKIREWMDEGQK
                     VVPISVNLSRMHFVNSNFIEKFKLIVDKYKIPTRLIELELTETAVLDNIEGLLDTMNN
                     LKEKGFVISMDDFGTGYSSLNLLKELPVDILKLDRAFFTEKDESNNEKIVISNVIKMA
                     KELKMKVISEGVETISQVEFLKQIGCDMVQGYLFSKPMPVKEFEKIAFKKE"
     gene            complement(26392..27000)
                     /locus_tag="ACOIAF_001523"
     CDS             complement(26392..27000)
                     /locus_tag="ACOIAF_001523"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HGZ2668537.1"
                     /translation="MVDNLIKYIHKSDVAIVVLHEIYGINSFIEDVCQKYYKYGYDVF
                     CPELLGEKRVFPYSKVQEAYDFFINNVGFDIYKKVEKLINDLKSEYKYVFVAGYSVGA
                     TIAWRCSEDSFCDGIICCYGSRIRDYMDVIPKCPTLLVFAKHDSFDVDSISCQLSRKQ
                     NIQIEILDANHGFIDRYSNNYSDIQTKVFTIKEKSFLSKCLA"
     gene            complement(27260..27805)
                     /locus_tag="ACOIAF_001524"
     CDS             complement(27260..27805)
                     /locus_tag="ACOIAF_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HGZ2668538.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEKNWADE
                     HRIGVAQGVDEEIIEGLRANFVGECTEVGMYLAMSRQADREGYPEVGEAYKRIALEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKKAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRYFGK"
     gene            complement(27923..29746)
                     /locus_tag="ACOIAF_001525"
     CDS             complement(27923..29746)
                     /locus_tag="ACOIAF_001525"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2668539.1"
                     /translation="MNLSTGNQKVSRLKKKEKPKDFKKTFKRIFMYFKKDKKITRFIF
                     LLVIIDSIISTVIPYTIGKVVDIIDIDTYSKSIFLKGIIILLSFYIIEFSVKLSKGIC
                     TARLSQSIVKEMRSNLFLKFKNLPITFFDKSSTGDIMSRVTNDVDNISTGISNSLVQL
                     ITGILNILMTFIMMFVLSPILTFCSVILIPIVMFVSNFIAKRTRVFFKQQQVELGKLN
                     AHIEEMISNINVVKSFNYERKSVNDFKEVNDRLLNISLKSQIYTSLLMPIMNVISNIG
                     FATICIIGGILASKDIITIGVIASFLSYVRQFTRPLNELANLFNTLLSAIAGCERVFE
                     IIDEQEEQEVHNMFKSEINDNKDGNLNSHFVKGEIELKDLSFSYDSKKKILDNINLKI
                     KAGTSNAIIGETGSGKTTIINLITNFYHIDEGSIFLDGKDIYSINRNYLRTCFGVVPQ
                     EGYIFNDTVMENIRYGNLEATDEEVIYASKIANAHKFIVKMREGYSTVLSDRGEDLSE
                     GQKQLISIARAVLKNAPILILDEATSNIDIATEAKIQEAINNLTYGKTSIIIAHRLST
                     ISNCDNIIVLENGRIVESGNHKELIKLKGHYYRNILLTQGK"
     gene            complement(29759..31531)
                     /locus_tag="ACOIAF_001526"
     CDS             complement(29759..31531)
                     /locus_tag="ACOIAF_001526"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2668540.1"
                     /translation="MRYFKKYIKKYIVLFLTAVFFLTLEAFCDLAQPTIMAKIIDIGV
                     SNKNLDYILSTGAIMIGITFLGAIFAIIRSVISARVSQHFSYDLRQDVFEKINTYSLK
                     QIDKFGRASLITRITNDINQMQQFVNGMMRVFIKSPIMCIGSLIIAIKLNLKMSIIIL
                     IAVLIIFIIILLNMKIGYRLFKNVQESTDKVNSKLRQFLSGIKVVKLFCRYDYENQSF
                     EELNDELFKNSRKATTMMSFFRPTITIIINVSIIIILLIGRHLIYSGEIKIGIIVAYV
                     NYMTRILTSLIMISHVFNVFVRAKASYERISEVLVDENEEVIEREESLDVGEKDLKFV
                     NCDIIFENVDFSYNKTDEKILKNVSFKIEEGQNIGVIGQTGSGKTSLVNLIQKLYIID
                     KGDIKIGGRSIYSISEESLRKRIGVVFQNSSVFGKSILENVVMGNRDICKEDVVKSCE
                     IANANQFITKLENTYDEVLFQRGKNLSGGQRQRITIARAIAKKPQILILDDCFSAMDI
                     NTETIIRKNIKEYISNLREKESKKCTVITISQKISSIIDCDKILVLDEGEVVGFATHE
                     DLLVSSPIYKKIFALQNLVSEDVN"
BASE COUNT        10462 a         5450 c         3171 g        12588 t
ORIGIN      
        1 ctatatctta tccttcagca ttatccgtta aggtctaccc acttccacta ccaatagctt
       61 gtagtgtact tccctcaaga ggaatagtcg ttgaagtttc tcctattcgg agtttacctg
      121 ctgattgccc attttaaagt acttaggatt taaccttata ccatctaacc aattttttct
      181 actttcgtaa ctttcacact taggtttatt tcatccttat gttttagttt gattagcttt
      241 aggggtttcc agcaattcaa gtagtattgg ataggttcta tcctatctct acatataggt
      301 ttccctatat gctgactatt ttagctacta actcatgact aaagtcacga gtgtgcgtag
      361 ctatttttta atcaatattt tagtaaattt tacggtaagt aaaaacctct ctccttcaaa
      421 tcctatagca aatatactta caagttgctt tgagaaaata actcctaata tagctacaac
      481 tatgcatata ccaactatta tatttaaaac attgtttaaa aactttagtg cttgcttttc
      541 accttgttta ctacttatat cttgatacat tggaataaaa gtagtaacta tagcagtacc
      601 tattgctgca aatataatat ttggtatatt catagctgtt aaataactct ctgtatataa
      661 gcctgtacca taaattgatg ataatactaa ttctctaccc attccaagaa tcttagagat
      721 tatagtaact atcatcaaat aaaaagttgc tttagctacc ttgctcattt tatcactcca
      781 cttctaacat acctcttttt aatcaagtaa aatttagctc attagatata attctatcaa
      841 ctaatttgcc ttgcttagcc ctatcaaaat ataattaaca cctatttctt aatatatcac
      901 aaactacatt attcttcata tttttttatt tctccaataa gttttacatt tttcctatat
      961 aaattgtata gcttcaatta tattcaaaga aatggagttg ctacccgata actttaaaag
     1021 taaaaaacag attttattat ctttttaaac tatattctaa catattttgt ccatattgag
     1081 aaattttact aaactattat gtactttatt aaataaataa aataacagca cacctataaa
     1141 atacaagtgt gctgttcata aacagatttt ataaaaaaat aattcctaag tagagattaa
     1201 catgctttct catactctca tatataatgt gtctaaaaat attaataaaa atgctttaat
     1261 tgttttaact tacttggatg tcttaatttt cttattgctt tagcttctat ctgtcttatt
     1321 cgttctctgg taactccaaa tacctttcca atctgttcta gtgttttagg gtcatcatca
     1381 tcaattccaa atctcattct taaaacttgc tgttcttgct ctccaagacc tgtaagtagt
     1441 tcttctatct tttcttttaa attatgctgt tcaacttcgt gtattacaac atctttaaaa
     1501 tcagcatctg atggtataaa ttcaactaaa ctactatcct catcttcttt taatggagta
     1561 tccaaagata caacattttt atctcttctt aataattcca acactttgtc aacttctaaa
     1621 ccacatgcat ctgcaatctc atctatagtt ggctctctta ataatacatt taaaagttct
     1681 tgtttctttt tctttacaaa atttattctc tgatgaagat gtataggtat cctaatagta
     1741 ttttcgcaat cgtcgatata tcttgttata ctttgtttta tccaccaagt tgcataagtg
     1801 ctaaatttgt atcctttttt ataatcgtac ttttctacag cttttataag tcctatattt
     1861 cctgcttgaa ttaaatccaa catatcaata ctatctcttt tatatctctt tgctatgctc
     1921 actaccaacc tataatttga atttataaat ttttcttttg ctactgaaga tgagttgatt
     1981 atttccttag ccagctctac ctcttctcct gctgacaaca ttttgtattc ctctatttcc
     2041 tttaaataca ttttcatcgc atttgataca tttgatgaac ccacacaaat ataattttca
     2101 taattatttt cttttttagt atccataatc aaaccccttc tattcataga atttgctaaa
     2161 acaaaaataa ataatttaaa cctacataat ctacaattac ataatatccc attctttaaa
     2221 tgaattgttt tgtatattat gtatacaaat ttataaaaac tatacctata atattattct
     2281 acttctaaca gcaatatcct ccttatatat ataatttatt ttattatatg ttgttatatt
     2341 cttagttttt tgaatgatat atgtctaaaa aaagactgtt tactaaacag tcttttttta
     2401 tatatatgct caatatttaa ttttcatgat gatgattaca tccacatcca caattttcat
     2461 catggtgatg atgttcttct aaatcttttg cctgttgttc catttcatga aactctttta
     2521 ccattatatc ataagttgga agagctgcct cagtatcgta ttcttcccct ctccaatcag
     2581 cgtacatcat agcatcccca ttagttaaaa ccaatctacc tgacatagtt atattatcca
     2641 cgtcttctaa aaattctatt tgtttttgta aaacttctcc ttctaactcc acatcttcat
     2701 ctaaacattg cactatcatt acaggtgcat aataatcttc ttcatcttta acatttacaa
     2761 caatatctaa aattccttct gcaccttctt caaattctgc caactcaaca tttgtatcaa
     2821 tcatatcttg aggtactaat aaatcttcta ttaaatattt tattactcta tcttttacta
     2881 attcttttga catttatttt tctccttccg attctataac attatcataa tcatttctat
     2941 attatagtat actacattaa ttgttttttc aatctcttac aaaaattatt tttttattat
     3001 cttccttata ttcccttttt tattacaaag ttgtaatcgc attgatttaa aaaatagttt
     3061 atatttagta taatagaaat gtagcacata tttttattca atatattaat aaaaaaatat
     3121 attgaaaatg taatgtatgt taaatctagg aggaatatta tgaagtttaa aaaacaaatt
     3181 agttctattg ttgtgtctac tatgcttgta cttgggagta tgaacctttc ttatgcagac
     3241 ggtacaaatg tagttacact tggagctaac ttatcagagt ctcaaaagca gcaaatgctt
     3301 aattactttg gagtaaaaaa agaccaagtc ttagttttag atgtaacaaa tgcagaagaa
     3361 agacagtatt tacaaggtgt tgcaacagag ggtcaacttg gaaaagttac tatctcttgc
     3421 tcatatgttg aacctactaa aaaaggaaat ggaattaatg ttaaaacagc aaacttaact
     3481 tgggtaacta gttcaatgat tgcttcaacc cttacaacag ctggtcttga agatgcaaat
     3541 gttattgctg ctgctccata tcctgtaagt ggtactggag cattgacagg aatcatgaaa
     3601 gcatttgagg atgcttcagg aaagaaactt gatgaaacta agaaagaaat tgcttctgaa
     3661 gaattggtag ttacaggtga cttaggagat gatatagggc aagaaaaagc aacaggtgta
     3721 atgaatgata taaaaacaga aattgtaaag aatggtacaa aagataccaa tcaaatagca
     3781 aatacaataa ataatgttgt aaataattac aatattacta ttacaccaga acaaaaagaa
     3841 caaattaaag gtgtaatgaa aaaaatagct gaccaaaatt acgattacaa tagcatgaaa
     3901 aatacactag ataatgtatc agataatgta aatgaacaat taaagaaatt aggtgaaagt
     3961 gttaaaggtt ctggtatact agatactatt ggtggatggt ttagtggaat aggagactgg
     4021 ttctctggtt tattctcagg cggagataaa aaagatttgg gaatcttaaa taacactaat
     4081 gatgagtctc ttggctctga tgctgtaata aattctacag aaggtgttca aataaatcca
     4141 caaaataata ctaattctaa caatgatagt gatacaaata gctcaaaaaa tgatacttca
     4201 agtaataatg aaaataacga tagttctcaa acaaatgaag acaacacaac aaataattca
     4261 gaaaatcaat caaataataa tgactcagtt ggtttctttg aaaaaataaa aaattggctt
     4321 tcaggcttat tttagaaatt aaacaaataa aaagaattgc ctaaaatgga aattttctat
     4381 aaaaggcaat tctctttatt tacaataaat ttttattact cactcatttt tttagatttt
     4441 tctatacatg cacatattgc ttttatcaca gaagctctaa aaccttcttc ttcaagtact
     4501 ttaactgctt ctatagtagt tccaccaggt gaacatacca tatcttttaa ttcaccagga
     4561 tgttttcctg tctcaagtac catttttgca gaacccataa cagcctgtga tgcaaactta
     4621 tatgcttgtg gtcttggcat tcctgcaatt actgctgcat ctgctatagc ttctataaat
     4681 aaaaatacat atgctggtgc agaaccactt gcaccaatta cagcttctat aagatactct
     4741 ggtactacct cagtatttcc aaaagaatta aatacttctg taactgcttc taagtcttct
     4801 ttgtttatat ttttatttat agaaagagaa gacatggctt catttacaag agctggagta
     4861 tttggcatag ttctaactat ttttttatct tctcctataa tagactctat atcttttata
     4921 gattttcctg ctgctattgt aactataatt ttattgtcat ctataaagtc ttttatctct
     4981 tccaaaatat catcatatat atctggttta acagctacaa ttactatatc agaggttcta
     5041 gttacttcct tcgagtcttt tgtagtattt attccaaaat ttgcatctat tctatcgaga
     5101 gttccttgac tatatgctga tgctataacc atattaggtt caactaattt tgaatttact
     5161 attcccccta tcatagcgct tgccatattt cctgcaccta tgaatccaat ttttttcact
     5221 ttagtacctc cttattgact aatagtaatc gttaaaattt aagcttcttt aactgcatca
     5281 gacttttcaa atggatttgg tatacttact accaaaattt tcactacctt attagtatag
     5341 ttgacccaat tatgattgtt cttttcacta ttatagtgta ttgtgtctcc tggatacatt
     5401 tctatttgtt catctcccaa aaacactgta agagtaccct ctaatacata cacaaattcc
     5461 tcaccttcat gtacgtaaca acaaatttcc tcactactct tacttggaag aatttcaatt
     5521 aatcttggta gcattgtttt ttcctttaaa ctagatgata aatgataatg tataaatgtt
     5581 gagttttcta catcaaatac ttctttttca taacttctta atacagctct tttatgctct
     5641 tttggcttca taaaaaagta agttaattct acatctagta cacttgctat ttttcctaag
     5701 gaatctgttg ccacacttgt taatcctctt tcaagttgtg ataaaaaacc aattgacaag
     5761 tttgtttttt cactcatatc cttcagcgtc atttgctttt gagttctgag ttgctttatt
     5821 tttgctccta tgtccttatt caaaaccatc acccctaaat aattttaatt ttttcataat
     5881 ataaatacaa aaatataaat ataggtactg aacctatctt tatttcattt aaaaaaattt
     5941 ttataagtat tactataatt tatcaatctt tttttgtcta catttttacg attatttacg
     6001 atttattttt cacatatgta tatttaatct tgccaactaa agatacattt tttataagca
     6061 tttactcttt atataataaa acttaagttg ttcatgtagt cttcttctgg tttaattatt
     6121 aaaatttcaa attatattat tatttctaca cattattaca tcctttagta caattataac
     6181 ataattttac aatttaatga tatctattca atgtcttttt ttactctaca acatatttta
     6241 ttttagagtt taatactata tatatttttt atatacagcc ttgtcatttt ttagattgtc
     6301 aagagttatt gaagtatttt atgtagattt agattttatt tcctaatttc tcatttaaca
     6361 agaattgctt atgataaaaa ataatatcat ctcaataaga ttcttgtaaa tatatctata
     6421 ttctaaatat aatttctttt tttgtcaaaa tttcttcatt ctttaataat taatttatta
     6481 cttataaaat aaactaatta tctatacaaa ttttataatt tttaataaaa tacacaaaaa
     6541 aattaatttt tatagctcat ttcgccaaaa tatatctttc tttatatcat taaaataatt
     6601 ataaatttat taaatatgtt tgtaattcta taaagttatt tttatcaaac taaaaattct
     6661 ataaaaaaat aagatacata cctataaaat accttcctat agatacatat cttacctata
     6721 taaaaactta ttctatataa aaacgccata aaaaatcttt tgcttcttct gcataatcta
     6781 tatttatacg tttactagtt tttatatcta actcatcctt actaaatact ttttttcctt
     6841 tatcatcata ataaaaatca cttatatata attcttcacc cattatactc ttactattta
     6901 gacttctatc tatttttaaa gctttgcaaa gttttcctgg tccgttagta atatttttta
     6961 cttgatattt actcaactca gtgtaagctt tgttgtatct attcatagat atctcatcta
     7021 aagaagttat cggctctacc cctcttatta atacacactc tggcacattt tctatatttg
     7081 cagataaatt taagcaatta tacataccat aaattaaata aacatatata tgtccaccat
     7141 ctaaatataa tggcttagtt ctatctgtct tcttattgcc atatacatgt gctcctttat
     7201 catttacacc catgtatgct tctgtctcta tgattttggt aacaattact ttattttcat
     7261 atttccttat tagatattta cctaatattg atttggctaa atcaattcca ttttttctaa
     7321 aaaaatcctt ttccaaaatt tcacctcata aaatttattt aaaacgcatc aaatacaact
     7381 aaagtaataa ataaaattaa ctcttattaa tgtgatatat ataaacaata aatttagaca
     7441 tttattattt atatatatca aaaaattcta tctcaaactt ttcaatatta tcattgtaat
     7501 ttgaattatc atatacttct tcaacaacct ctttaattgg gaatataatt tttacactag
     7561 ttccttctcc taccttacta tctatgtaaa tcttcccatt ttgaatttca atcaatgatt
     7621 ttgtaaggca taaacctata ccacttccaa attcatttga tataccactg ttttcaattt
     7681 gtttaaatcg attaaatata tcatttaatt tttctttgtc tatgcccact ccagtatctt
     7741 ttataattat ctctactgtt tcgtcttttt tatatatacc tacaaggatt tctcctcctt
     7801 ctaaagtaaa ttttatagca tttgataata tatttagtat tattctttct atcgattctg
     7861 ggtcaagccc taccataaca gtttcttcat ctgtatcaaa aattaaatct attcctttag
     7921 acttagcata actactaata gatgtaacta ttctttctgt tatgtcaata atatcaaaat
     7981 taactgcttt aatcttatat atgtctgact taacttgaga aatatttatt atattatcta
     8041 ttaatctcaa taatctaaac atattttgtt tagttatttt tatatgttct tttagtatat
     8101 cctgaaaatc aattattttt ccttttttat aaaggttttc aataagttga actgaactat
     8161 atatattatt taaaggtgtc ttaagttcat gagatatatt agcataaaac tccatcttca
     8221 tttggtcata ctgtctttgt tttattaatg cttttttaag tctttcagac tctctttgca
     8281 cacttaaatc tttaataatt ctcactctac ttcttttctg tccaaagtat aatgttcttg
     8341 atatcatttt tacttcttta atgtgattat tttcatttac tatatggata ctcttctctc
     8401 tttctcctat atttaaattg tttatgtatt tttttactaa tatatttcta tatttttgtg
     8461 ttgtaactcc taatacatca ctagaaattc cgaacaactc tctaaaggct ctatttgtat
     8521 atagtactct tgatgtactt aaatcctcaa gaaaaatagc ctctggtata acatcaagta
     8581 atttttcata tattattttt tgttcattta ctgttctaag aatatttatc tctttagtta
     8641 tatctctaaa tgtaagcaat ttaaagagtt tattatctga ccaatagtct ccaactctaa
     8701 cttcttcaac atgctcttca ccttctgagt cagacatcac aaaattaact atacagtttg
     8761 ctgtgtcatt gtaacttact atttcagctt tcttttcttg tgttagattt aatcttttaa
     8821 actccctatt gtcaaagaaa tctatgttaa aagtttcaaa atattgttct aatgtcatat
     8881 cgaccaaatc acttgaaaca catttaaaac tcttctcata ttttttattt atacttatta
     8941 ccttattctt ctttatacca ccttcagcac ttcttacaag tataggagat ggaatattta
     9001 gtataatgtt ttcatagtct ttatttatat ttcttatttc ttcatctatt ttgatttcat
     9061 ttgtaatatc atttaaactt attaagtaat tgctttcatc tttattatta cttaaactta
     9121 acaaataaat tttatcattt aactctacca gtttatcaat tatacttata tcgttatcaa
     9181 atttcttcaa tttttcagca actttttcat cttcctttat aaaagttaat attttataat
     9241 aattattcat atcctttgaa aattcatctt taaatttatt atttgtgtaa cttatattaa
     9301 aatcatagtc tgttactatc catacatttg gtattgtatc cagagcttgt ctaagtaatt
     9361 cttttctctg gtctatgtag ttttttaatg ttctttgtat aatcatatcc ttttctattg
     9421 ctaaatttat ctttcctaat tgctctgact ttttacttaa ttttttattg attgaatcaa
     9481 gttcataata tggatacact actctattat taagaccgta tacatataca taataagtat
     9541 ggttacataa tagtaaatat attactccta ttaatctatc tgaatctact atattaatct
     9601 tagaaattgc tacaaaacat ataatagttg taaaaaaaac aaaatatgtt aaatctttta
     9661 ttaacttata gtgctcaatg ttactatcaa gtttattcaa caaatatagt aaattaaaca
     9721 tagatataca aatattaatt aatataataa tcatattcca gttaatatca ctaaaaatat
     9781 ttcttataaa gtaatctaat atcacaaaag cactataaaa aattagtatt gggcttattc
     9841 ttagtttttt tactttagaa atggttaagt atataaaagt agacatatta attatgctta
     9901 agttaagcaa tacaatttgg aatttttgaa tttttaaatt tactactcct aaaatactta
     9961 caaaaactaa tacaagacac cctactttca atatatttat aaattcatct gttttaaagc
    10021 tacttatata aactatagaa tatccaaata tccaaagtaa aattattcta tacatattta
    10081 tagaaactgt atacaaattt atatttaaaa gtgcaataac caaaaataac ccattagcta
    10141 gtatatagac cttattctta cttaaagttc ctttagattc catattcccc accacttctt
    10201 catttttata tagttttacc tataaatatg ttaccacaat tcttccaaaa aatttgtcac
    10261 tttctagtta taaaaaaaaa tgagtaaata tttttaattt tactcatttt gtagattttg
    10321 tatattcagt ttaaaaagct ggaactatac ttcctttata ttcatcttgt ataaattttt
    10381 taacttcttt actattcata gcctctgata aagccttaat cttatcacta tctttattat
    10441 tttctctaca agctattata tttacatatg gagaatcact tgattctatt actattgcat
    10501 ccttagtagg atttaagtta gctgttaaag cataattaga atttattact gctccgtcaa
    10561 catcttttaa tactgttggt aattgctctg cattcatttc ttttattact atatttttag
    10621 gatttttagt tatatctttt ttagttataa gctctccatc ttttacttct atcaatttat
    10681 tttttgctaa taatttaagt gctctagctc cattagttgc atcatttggc acagctataa
    10741 cagctccatc ttttaattca tctaatgact taactttttc tgaatagaat cccattggct
    10801 ctatatgaac tttagatgta taagtaagtt tatatccttt ctccttcaca gtttcctcta
    10861 aaaatggaat atgttgaaag aagttagcat ccaaagaccc ttcatctaaa gctgtatttg
    10921 gaagtacata atcatcaaat atttttactt ctaaatcata tcctttttct tttaacaaag
    10981 gttttgcaac ttccaatatc tttgcatgtg gatttgatga agctcctact agtatttttt
    11041 tatcctcttt gtttgaacaa cctactgctg atattgctat tgctgacacc aatgctactg
    11101 ataataattt ctttaatttc atttttactc cccctcttta aaatgtttat aattaattta
    11161 ttttagtttc ttataagcca gatttcctac tccctggatt atctgaacta atattataag
    11221 tgctattaca gtatatacca ttactgctgt atcaaatctt tgatatccat aaataagtgc
    11281 tatattacct aatcctccag ctcctactgc acctgccata gctgtataac caagtataga
    11341 tattattgat aatgtaattc cagatactat tgaaggcata gcttccttta tcataacttt
    11401 aaatactatt tgtctttttg tagctccaaa agattttgct gcttctatca atcctttatc
    11461 aacttcattt aatgagcttt ctataattct agcgataaac ggtgctgccc caatagttat
    11521 tggcactatt gcagcagttt cccctatact tgttccaacg attaatctag ttattggaat
    11581 cattgcaact ataagtatca taaatgggaa acttctaaat atatttacta taaatcctaa
    11641 tgcactattt attgtgctat ttggttttaa tccatctggt tttgtaacta ctaatatgat
    11701 tgctaatata aatcctaata ttgttgccac tatagttggt actataacca tatacaaagt
    11761 ttgtaacaat gcatttggaa atagtgttgt taaaaagtca attaaactat tcatttatca
    11821 tttcctccca tcttatgcct ttactagtta gatattgttt tacttgctct ccacttttat
    11881 ctgaaatgtt tataataagt gaacctaaaa tatcatcttt aaattgttct aatttcccaa
    11941 atattataga cacatctata tttagttctc ttgccatagt tgtaataata gcttcattag
    12001 aaatatcttt agggaatagt atctttatat ttgttccttt tggcaatatt atactttctt
    12061 cacctattaa ttttctaagt cctttagtat ttcttaagaa tatttcttct gtatctccta
    12121 cctctaatac ttctccattt tccattattg ctactcttcc acaaatctgc tttataactt
    12181 ccatttgatg agttacaact attattgtta ttcccaattt tttatttata tcttcaagca
    12241 atgaaagaat tgactttgta gtatttgggt ctaatgctga agttgcttca tcacaaagaa
    12301 gtacttgtgg atttagtgct aaagctctag caattgcaac cctttgtttt tgtccgccac
    12361 ttagatttct gggtttatca tttttctttt cagatattcc aactacttct aataactcta
    12421 aaactctttt tttaatttct gcctttgagt atccaaaaca ttctaatggc aatgctacat
    12481 tatcaaatac agtttttctt tcaagcaatg aaaagtgttg aaaaatcatt cctaactctt
    12541 ttcttaaatc tctcatttgc ttttcattca atgactttac ttctttatca gaaacaagta
    12601 cactgccttc ttggtattct tctaatccgt ttatacatct cagtagtgtt gatttccctg
    12661 caccactatg acctattatt ccaaaaattt ctccagattc tatttcaata cttacatctt
    12721 taagcacttg aatctttcca taatacttat taacattttt tatgctaatc ataaaatttc
    12781 ctcccatata ttctgctcag tttttatgtt tatcctctct tatgtattac aacaaaataa
    12841 aagcccgagt ataaactcag gcttagaaag ctatttatac tcatctatca actatattag
    12901 ttgcaggatt tagcaccaat gtatataaaa tatactggtt gccgggtttc aaagggccag
    12961 tccctccacc actcttgata agacgttatt taatttaatt ctattatcat ttgttgctat
    13021 gttatatatg atatattcat taatagtatt tgtcaataaa tttatttaaa ttttttattt
    13081 tttattatcg ttaattaact atttttaatt agttttctag ctcctaacac tttatatttt
    13141 aatgtctata taactataga acatcattat aagaaataaa ttaaataaca tcaatctatc
    13201 taaatcatct gtaaagtaaa ttaagaaaat taacatttta tccaaactct atcctactat
    13261 taaataaaat aatttaaata caatgatgat tatatagctc tagtacttta gactttatca
    13321 aatgtttaag ttctataggt tctattattg tagcattttt cccaaaagtt aaaaaatatt
    13381 cagccaccca gttaagtgta gattttggta tatacatatc tatgtatcca cctttagaca
    13441 agattttcaa ccctcttgcc aacaaaaatt ctgtttcaca acgttttact ccctcatctg
    13501 tcaaattaat tttaatatga taatctgtac ctacttccat attcttaaga taatcatgta
    13561 tagaagataa aaccttatat ttcttacttg ataagttttc tatactcaaa atcttcacaa
    13621 ttctgtctac acgaaattcc ttaatagatt ctgattttat acagtaagct ggacaatacc
    13681 aaagtccatt catggcatac aaaccaatag gaacaatggt acgtacacta tttttttgct
    13741 ttgatgaata ttcaatttta attgtaaatt cattcattac tatattaaac aattctttca
    13801 ataatggtgt tgaacagtgc ctatctggtg tccaaaaaac aaatttacgc cttatattct
    13861 caatattata ttgaatatca tttggtatac aatttaaaaa tttttttaaa acagaaatag
    13921 tttcttgctc aaatggcaaa tcattataat attctagtga ctgatatgca aagaatatag
    13981 attttacctc actttctgaa aatgcaatcg gtggaagtat acgctcttta agtacatgat
    14041 atccaccagc cgctccaact tttgaatata atggaaatcc aatactctcc agcacctgta
    14101 aatctcttag tatagtacgt tttgatacag aaaattcttc tgctaattct cctgctgtaa
    14161 aacttctttt tgcatttatt gttatcatca gttcaattaa acgctcagct cttgacatat
    14221 ttatactcct aatttaaata tagtgacaat aattgtcact attatatgat aagatattct
    14281 tgcatctaaa gcaataataa tagtaaagaa agaagggatt gatactttat ggaaaatgat
    14341 actattaagg ctgatgatat tctcaattat tgtctatcaa acttagatga tgttgtacta
    14401 atggatagtt ggggggaacg agcaatttac tacaatccta atggtgtttt aaagcgaggg
    14461 gtatatgttc ttaccattaa ggaaaaagac agtaataatg ataaaggttc gttagttagt
    14521 cgtccaaatg tataccgtgt gaatatagga ttaaaaaaag aaacttttat tgaaatgttt
    14581 ggatatattc caaagcgtcc aggtgtaggt caaatagttg atatggattt tgattttaca
    14641 aaattggaca caatcatgcc acatcctatc tactcatgga tgggatggat atgcgtccta
    14701 agccctactg aaaagacatt tgagaacttt aaaactttaa taggagaatc ttacaatttt
    14761 gcaaaacaaa aatttaaaaa gagaaaaaac aagtaaaaac atatgaaaat aatccctaaa
    14821 taaattataa taaaaatatt aaggttacaa gataaaaatt aatatcttaa taacatgcaa
    14881 aaaagcgaag ccaaaaaatg tattatttat aaggcttcgc tatatctcaa ataatttatt
    14941 tcaaatttca aaactattta ttctattatt tcttattatt tcctgttatt cctaatctat
    15001 cattcatatc ttttatcatt tttctaaaac cactacctat atcatttcca acatcttcag
    15061 ttccatgaac taattttata aagtcatttt gacctttttt catatcttca tcagatacct
    15121 tatctaattt ttctattctc ttatctatgt cttttactaa tctttcccct tcagcgatta
    15181 ggtcatcatt cattttcttt aactctgtat cttctgtttt accatctttt ttaagagatg
    15241 ttacaaatga ttgtatttta tctctactat ctttatatgc agcctttaca tcacttagat
    15301 atttttcatt tcctggatat tctttatctt taaactgatt atttatactt tctggagtat
    15361 cataaataga atatctattt agacctgcta tataatcatt atagcttgtt gaataataat
    15421 tcatatatcc ttcattatta tttacagatt cattaggatt ttttcctgta ttttcacttg
    15481 gtttttcatt atttttagta catgcagtta tacctatagc taatactagt gctaatccta
    15541 caaataaagt tctttttaat ttcattttta ttaccctcct agatgtttta tactataaaa
    15601 aattttaata attttctttc ttaatttagt atttctatat ttttatattt tatttactaa
    15661 aattaaacaa gttataattt atttttttgg aaatcataat gaataaataa ttaaataaaa
    15721 aaattataat tttattattg atattgatta tcattcatag tataatctaa ttacttgata
    15781 ttaattttca tttgaaggag gttatctttt atgtcttgtt gtaattcaca attttgttgt
    15841 aaaaataaaa ttgaatcatc atatataaaa tggcttttag aagttttagg tccagtcttt
    15901 ttaggctcta aacctgctga aataataaat atttcgttta ctgatgtaaa tagagaggaa
    15961 aaaattaatg atatttataa atatctatat aaatgtaaga aaatagattt tatagttatt
    16021 gataaagaaa aaaagggatt aaaaatctta tttgtaaata aaaaagcatt atcagaaaaa
    16081 ttaaaatgta aaaaaacagt aaacttttta aagtttttag gatacaaaca aaatacgaat
    16141 gttaatgctt atttggagca tttagtagat aaacttaaga gtgatgtttt tcctgatgaa
    16201 ataggtattt ttttaggata tcctcttaaa gatgtgattg gatttatggg atatagtaat
    16261 tatgaagttt ctatgattaa atattggaaa gtttatggtg acacaaaaca atccgaggac
    16321 acatattcga agtttttact tcaccgtaag aaaatgagaa aattactaga ctacataagt
    16381 gtagataaaa tagtaagctg tttttaattg tgtttttgta taataataaa aatacaccct
    16441 cttttaccat acagtttcca taataaataa gggtgtattt tccactttaa tattttaaag
    16501 tattctaatt ttgattgtac aacatatctt taatatttat tttcttttgt tgcattccat
    16561 tagacatcaa aaattcctgt gtatcttcta attcttttat atccttgtcc gttattttag
    16621 aactaaagtc ataccaagaa tacatttctt ttgtttcatc taaagataaa ccaacttctt
    16681 tagatacaac atccatcact tcatccttgt tttcattcat atattttaaa gtttcttcat
    16741 gaaccttcat aaatcttttt actaattcag gatatttttc agcaaagtca tcacttacag
    16801 cagtaactat tattccactt actaatcctt ctccatttac aatcattttg gcacctgaat
    16861 ttatcgtttt caatgccact ggacctgcaa gcattgctac atcagcactt ttactttcta
    16921 atgcagaagc agcactagga atatccatat ttacatattc aacatcatct atagtatagc
    16981 cttctttagc aagtgcagca atcaacagtt gatgaagtat agtccctttt ggacctacaa
    17041 tttttttacc ttttaagtct tttatagatt taatattatc attattagtt ataagcatat
    17101 atccttttgg tgaacggcta taaatattcg taattttaag accaacacca tttgatgcag
    17161 ctattattgc agacgttcct cctaatgcat gtaaaaagtc tagctcacct gctgctaatg
    17221 cttgagtttg ctctggtcct gttgtcaatt catggaagtt gacttttata ttgtcctttt
    17281 taaattcctt tccaaataaa tcatcctttt tttctaatat actaggaaca ttaagtggtg
    17341 attttacata agttaaattt atttccttag gtaaactagc ctcttttttt gcaggttcat
    17401 ctttcttatc atttgttgtt gaacatccta cagtcccaac caacaccatt attaaagatg
    17461 tcattactgc taaaatcttt ttgtttcttt taatcaatta aaactccccc aattccctta
    17521 atatttcttt tttcaagttt atataatagt ttttagttaa atctctatta tattcatctt
    17581 cgacactaaa ttgtttaatc cttttatttt tactaaaaac aattattttc tttgctattt
    17641 ccatagcttc ttcaatattg tgagttacaa atactacacc ttttttagta tttttatgga
    17701 tatttacaac ttctttttgc atttttcttc ttgtgaagta atctagtgct gcaaatggtt
    17761 cgtccatcag caaaatatcg ggattgaagg aaagtgctct tgctatactt actctgtgag
    17821 ccattcctcc tgataattca tttggatatg agtttttaaa cttttcaagc ttcatcattt
    17881 ttaaatactt gtctaaatca actttatttc tattatcttt ttcagtatgt aaaagtatat
    17941 tttcacttac attcagccat ggcataagtc tgctttcttg aaataccatt cctacttttg
    18001 gagtacactt tttatcatta ttaaaaaaat atatgtttcc actagtagca ttttcaagcc
    18061 ctgctaaaat tctaagtagc gttgttttcc cacatccact ttcacctaat ataacagtta
    18121 tctcttcact agatatattt aaagatatat tatcaagaac taaatgctct ttattatcaa
    18181 ctaaatattt cttatatata ttctctagca aaaatccact tctaaccatt taaatcaacc
    18241 tgcttccctt tgctaaattt tgttattatc tttgaaaata gataatctga aagtatacct
    18301 aaaaatccaa taacaaaaat tcctaccaat acaacatcag ttctagacat atcttttcca
    18361 tcagaaatca aatatcctag tcctgaagaa gctgcaataa gctctgctcc aattatagcc
    18421 ctaaaactat acccaagccc taaactaagt ccaacaacaa tatccagcat agaattaggg
    18481 attattattt tataaaaaat tctcttttta gataaattaa aagatttacc tacttctatt
    18541 aatttattgt cacaattttt tatacctttc aatgtattta aaaatattgg aaaaaaggat
    18601 gccaaaataa taattataat tttagattct tcacctatac caaaccataa aataagcatt
    18661 ggcaccaaag ctagtggagg tgtacttcgc ataaattcaa atagtggttt gaaatattca
    18721 tacatttttt tatttactcc aaataaaact ccaagtggta tagccatcaa tgaacttatt
    18781 aaaaaaccta ttaatatcct tcttacactg gcataaacat ttaagaaaat agaaccgtca
    18841 tatatcattt ttataaaagt atctaagact atttcaggag gaggcagaat atagctactc
    18901 catataccca aaacacatac tatcttccat aaaattaaaa ttattaaaaa aatataatat
    18961 cctttaagcc ttttatgaat tttctcaaac atataaaact ccttttaaca tatctatccc
    19021 tgctaccctt tttatttttt aatatcttaa ataataatta agctatatca taagatttaa
    19081 tctatgccaa gtcttaatcc acctgaactc attctatcag aaggtgtaga atctgttttt
    19141 aagccagaca atctttcaga tggttgtata atgactattt gtccaggttc aacaaattcc
    19201 atatgataag attctcctga agtctgacca ataaaagtct tccatgatag ttttgatact
    19261 ttaggatatg gttctcttcc agtccaagct actaatgcat ctgggtcaac acaacaaggt
    19321 ccttcaagaa ttagaggatt accatctgtt attatagcaa catcttgatt ttgacctgtt
    19381 ttatttacta aatgtgtagt aaaaagacct ttttgagata ttacccctga accaatcaat
    19441 tttacactgt aatttaattg ttcagaaaat gccaataagt tttcactctc aacataaatg
    19501 gaatcacctg ggtcaattga tattacatct acatggtaag cattttgagc taggtaaatt
    19561 tcaccttcac cttcaaccac cattattggc atttgttctc ctgtcaatga cctctgaaca
    19621 tgacctaata atgctcttcc caatccaccg ccattacttg gacctaacag caatttttca
    19681 aaattaaaat ttcctttaaa ggctaccata gcaccttttt ttgcaaaaaa tttaccactt
    19741 ccccttgcta tacacataag ctcgtttgta acatcaaatt taaacataat attcacttat
    19801 attgcttaat ttagcaataa tccctcctaa tcatatattt atatataatt atacagtaaa
    19861 attacagcat agtctaacct acttcaaata tgaaatttgt tatttataca aaaaataaga
    19921 acctgataat aaagcaattc aactttttat caggctctta tcctatttat ctcttagcgc
    19981 tatgacaact cttactactt gagcatccag aacaaccaca tccacaactg ctaccattct
    20041 tttttgaatt tttaaccata tacattacag ctagtgccat taatactact acaatagctg
    20101 ctataataaa tgtagccata ttcatcactc cttatttata tgaattaatt tttcatataa
    20161 cttaatttac ccattttttc ttttttcata tttttatttg aataagttaa aactacaaag
    20221 actactgcta ctgctataaa tatagatatt attgccccag caatacttcc tccagtacct
    20281 aaaactaaac ttccaacttg atttataaga agagcaatta tatacgctgt taaagtttgg
    20341 aatcctaatg taatccatgt ccatttccat gaacccattt ctctttttat ggctccaata
    20401 gctgcaaaac aaggtgcaca aagcatatta aatgccataa atgcaaacgc actagctact
    20461 gtaaacatgc ttgcaactga agcaagaagt gttgggtctt gctctgttgc atctgatata
    20521 ccaaataaaa ctccaaatgt tccaactaca ttttccttag ctactagacc tgtaactgtt
    20581 gctactgatg attgccaatt tccaaatcca agaggagcaa atattggtgc aactatattt
    20641 cctaagctag ctaatatact atctccagca tctaccattt gtaatgacca attaaatgat
    20701 tgtaagaacc agattactcc acaagcaaca aatataattg ttcctgcttt gattataaat
    20761 gcttttcctc tatcccacat atgtattaaa acaccttttg cacttggaat atgatattga
    20821 ggtaattcca taacaaatgg agatggttct cctgcaaata aactagtttt ctttaatata
    20881 attccacaaa tgattatcat tgcaatacct aagaaataca ttgatggagc tacccatgaa
    20941 gcacctccaa ataatgcccc tgcaaataat gctattattg gaatcttagc tccacatgga
    21001 atgaatgtag ttaacataat agtcattttt ctatctctat cattctctat agtacgagtt
    21061 gacataactc ctggaactcc acaacctgaa cttataagca ttggtataaa tgattttcct
    21121 gaaagaccaa acttacggaa aattctatcc attataaaag ctacacgtga catgtaccca
    21181 caatcttcta atattgataa taataagaat aaaagcatga tttgtggtac aaatcctagt
    21241 actgctccaa ctccaccaat tagaccatca actacaagtc cttgtaacca ctctgctaca
    21301 tttaaagaag ctagaaaatt acttacattg ccttgaataa tttctccaaa cagtacatca
    21361 tttgtccaat cagttgctat agtacccaat gaacttactg ctatatagta aactccccac
    21421 attataagtg caaaaatagg taatgctagt atacgatttg ttacaatctt atctatctta
    21481 tctgatacag tttccttacc ttttcgattc ttctttatta tacttgaaat tattgaacta
    21541 ataaattcat aacgattggc tgttattata ctttcactat catcatccaa ttcatcttca
    21601 caatttctag ttatttcttc aatactattc aatattgttt tagaaatatt aagtttttgt
    21661 ataacatttt catctctctc aaatagcttt atagctaacc agcgtgtttc tacatctata
    21721 tatggagtct tttcttctat tattttttca attttttcta ttgcatcctt actttcatca
    21781 acaaagggta atttgaaatt taatttattg ttactacttg caagttcaac tgccttttca
    21841 gcagcttcca taattccttg acctttaact gctgttactt ctaccactgg gcaacctata
    21901 acttcagata gttctttaat atttatctta tcaccattct tatttacaat atccatcata
    21961 ttaagagcta taacggtcgg tatacctagc tctaaaactt gagttgtaag atataaattt
    22021 ctctctatat ttgatgcatc tacaatattt attactgcat ccggcttatc atctagcata
    22081 aaattacgtg taactacttc ttctaatgta tatggagata aagaataaat acctggtaaa
    22141 tctactattt ccacatcttt atttcctttt aattttccac cttttttttc aactgttact
    22201 cctggccagt taccaacata ttgtgatgag cctgtaagcc cattgaacat tgttgtctta
    22261 ccacagttag gatttcctat gagacctatt tttattgaca tttccattcc ctccattatt
    22321 atttaaatac atcactgttt gtgatacaga aaatcattct cattatataa ttacattaac
    22381 cctactctaa ttaatcttta ttattccaca ataatttttt ctgcatctga cttacgaatt
    22441 gatagttcat aatctctgac tgttacctca attgggtcac caagaggagc tacttttctt
    22501 ataaatatat taacacctct tgtaatcccc atatccatta tacgtcttcg tgtagctcct
    22561 tctccttcta actttttgac ttttacagtc tctccacact taatatcctt cagtctgttc
    22621 atgtcattac ctccctatat tattattcta cttgccattt ttttatctaa tgcaactcta
    22681 gtatctttta cgtctattat taagttacca tctatcttag aaactatttt aacatttgta
    22741 cccactacaa aacctatact atttaaaaaa gtcctcgtct tatcacttcc cgaaattttc
    22801 tttataacta cttcttcacc tgtatttgat aatatcaatg gcatcatata atatccctcc
    22861 tcttaaagta atttaaatct acaagcaatc gttatcttat atataaatta caatacctta
    22921 aatcttaatc tattaaaata atatcctatt ttttccttct ttttacaatt cctaattttg
    22981 atttaagtct atcacaatag tatatttttt gcaagtgatt attgtattct tttgattttt
    23041 tttctcattt gatttatttt attaataaaa tcgtaaaaat taatacaaaa ataaagatgc
    23101 tctaaaatag tatactcaac taatattaaa gcacctttat tatatatgtc attatttaca
    23161 cattgcttta ttctttttta aaagcgattt tttcaaactc ttttacaggc attggcttag
    23221 aaaacaaata tccttgtacc atatcacacc caatttgctt tagaaattct acttgcgaaa
    23281 tagtctctac accttctgat attactttca ttttcagttc ctttgccatt ttaatgacat
    23341 tagatataac aattttttca ttattactct catctttttc tgtaaaaaat gctctatcta
    23401 gttttaatat gtcaacaggc aattctttta aaagattaag agaggaatat cctgtaccaa
    23461 aatcgtccat agagattaca aatccctttt ctttcaaatt gttcattgta tcaagtaatc
    23521 cttctatatt atctaatact gctgtttctg ttagctctaa ttctataagt cttgtcggaa
    23581 ttttgtattt atctacaatt aatttaaact tctctataaa attactattt acaaaatgca
    23641 ttcgagacaa gtttacagat ataggtacta ctttttgtcc ttcatccatc cattctctaa
    23701 tctttttaca cacttcctca aatacataga aatcaatatt tacaataaaa ccattcctct
    23761 caaatacagg aataaattct attggtggta ttagaccttt ttgtggatta tcccaacgaa
    23821 caagagcctc tgcccctata atttgataat ctgaaagact atatttaggt tgaaaataaa
    23881 caataaattc tccattatta agagcatcta ccattgaatt ttcaatttct ttctctttta
    23941 atattttttt gtgcatttcc ttatcataaa aagcaaatga atttttatga ccacctttta
    24001 tagtttttct tgcagtatta gctctatcca taatagtatt tatgtcatta tcacttttac
    24061 ttattttata tataccacaa tctaaaatca atttataata gtttatttta ggattactaa
    24121 gtatttgaac cttattgtaa atattgttta accttgtttt aatatcatca tctgtcttat
    24181 acttaataag aagaacaaaa tggtcagcag aaactcttgc aaaaatttca tcttccttta
    24241 attcacttgc aatagtattt gatatatgta tcaaagtatc atttcccata tcatatccaa
    24301 acatatcatt tatatattta aatttgtcaa catctatata aaacaacgca tattcttctg
    24361 gattattttt atcaaacagt ttattagaat ttattttaaa tttatctata ctatctgctc
    24421 ctgtcaatgg atctgtatat gctatcttcc ttagataatc ctctttcttc tttttattat
    24481 aaattatata tttaatagca attaagaata aaatacatac aaaagaaata atttgtattg
    24541 aatattcttt aaaaatcaca ccaaatggta ctttataagg tttgtcaata gtattagaat
    24601 atatacaatc acttaattct tcttcagtaa ttttgtctat tgatttattt aaaatagaca
    24661 ctaatatagg gtcagtttta tttgataccc ctatacgcat cccatagctt gtatctaaaa
    24721 cagatataac agataaattc tcactctttc cttctcttaa aagttcatca aactgataag
    24781 agctaagtat cattaagttt gctttacctt catttatagc gtttagacaa ctttctacac
    24841 tcttataaga tgttgtctta gcattttcaa aactcttttc tatataagaa tcaatagact
    24901 tataactact agttaaagca atatttaaat cattattgag attggtatcc aattttccta
    24961 ccataatcat tggaatattt atgtatggat ttgtcaattt catattaaat cgttttgcct
    25021 tatcattttc attgattgag ccacatagca tagttgcctt accacttttt atgtaatcaa
    25081 gactctcatt ataatttttt gttttaatat attcaaattt tataccacat ttttttgata
    25141 taattgacat taaatcggaa cttatacctt taaatgaatt gctttcttta tcataatact
    25201 ctacaggaga ccactctgaa tcatatacca tagtaagttt tgggtttgct ttaataaaat
    25261 tcatttcttg ccgagtaaat gctatggaat tattgacctc tcttttgaaa tattttttat
    25321 ataattccaa ttcataatac atatcattta tttttaattc ttttaaagca taatctaatt
    25381 cttttaccaa attaggttta tcttttgcag tagcaaaata aaaaggttct actgaaaatt
    25441 tgctaactat tttcattcct ttagcattta taatacttcc acaaacaaat gcatctgcct
    25501 ttttttcatt gacggactca attaattcat caatagaatt acagtatata tattcaactg
    25561 aaaaattatt ttcttctcta tacttctcaa atgcagtatt atgaaagttt cctctaagta
    25621 cagctactct cattccctta aaggaactta tatcttcata aaacatatta ttgttactat
    25681 cctttgtata tagtgcacta tattcatatc ctgaacttag tttaggaaaa ttaaatagtt
    25741 tatttctttc atcattcttc tgtaaaggtc cgagtaagtc tatttcaccc ttttctagtc
    25801 tgtctaaaca ttcttcccaa gaccctttta caaattcata ttcccatctt gtatattttg
    25861 agatttctct tagataatca taaccatatc ctgtataaga atgctctttt gatgaggata
    25921 ttattccgta gtcatcacaa tatccaactc ttattttttt tgaattttca gactcatcag
    25981 caaagcatag tatattgtta aaacatgata taataagtaa aaatatcaaa gaaagtatca
    26041 agaagtgctt tttaaatgct ttcatattta aaaacctcca atttgccaac ttactttaac
    26101 ttatttggtt ttctagttgt aactactttt attgtaatgg taaatatatc aaattgcaat
    26161 attctcatat aatattctta ttcaactata ataaatttat tattatctat ttaaatacac
    26221 tatctaatat tagtttctta taagtcataa aagcacaact aacactataa ttctagtgat
    26281 taattttatt tcaaaaaata taataacact actaatattt taaatatcaa tagtgtcata
    26341 aaaacttttc agattaatta aatctttcta tatattttat aataatctaa atcatgctaa
    26401 acatttagac agaaaacttt tttctttaat agtaaatact tttgtttgaa tatcagaata
    26461 attatttgaa tatctatcta taaaaccatg atttgcatct aaaatctcaa tttggatatt
    26521 ttgtttccta gaaagttggc aagaaataga atcaacatca aatgaatcgt gctttgcaaa
    26581 tactaataag gtaggacatt ttggaattac atccatgtaa tctcgaatcc gagagccata
    26641 acaacaaatt atgccatcac aaaaagaatc ttcactgcat ctccatgcaa tagtcgctcc
    26701 cacgctatac ccagcaacaa atacatattt atactctgat tttaaatcat ttataagctt
    26761 ttcaactttt ttataaatat caaaaccaac attatttata aaaaaatcat atgcctcttg
    26821 gacttttgaa tatggaaaaa ctcttttttc tcccagaagt tcaggacaaa atacatcata
    26881 tccatattta taatactttt gacaaacatc ttctataaag ctattaatcc catagatttc
    26941 atgcaaaaca acaatagcta catcactttt atgtatatac tttatcagat tatccaccac
    27001 ctttacaaaa cagcaaattt tttatactta gtttaatata ttcttcttta catgtcaatt
    27061 tacttttcta aaatcaaatt tatttttgga ttaatcattt atttttaaaa gttgtgcatt
    27121 attactatta aattattctc tattattaaa aatataatcg ttagaaataa aaaataaagt
    27181 acaaataatt aaaaaatatc tcactcataa ctttaaaaat tataagtgag atactcctaa
    27241 aatctacttt atattaaatt tattttccaa aatatctatt taataatcct aagaatgctt
    27301 taccatgtct agcttcatct ttacacattt catgtactgt atcatgtata gcatctaatc
    27361 ctaattcttt tgctttctta gctaatttta attttccatc agttgcacca tactcagcat
    27421 caactctaac tcttaagttt tcttttgtat ctgcaactac aacttctcca agaagttctg
    27481 caaatttagc agcatgttca gcttcttcaa gagctattct cttataagct tctcctactt
    27541 ctggataacc ttctctatca gcttgtctac tcattgctaa atacattcct acttctgtac
    27601 actcaccaac aaagttagct cttaatcctt cgattatttc ttcatctaca ccttgagcta
    27661 ctcctattct gtgctcatca gcccaatttt tttcgccttt catttcttca aatttatcag
    27721 ctccaacttt acatactgga cattgtgctg gtgcagcatc tccttcatgt atatatccac
    27781 atactgtaca aacaaatttc ttcataatta ctttcctcct aaattttata tatcaaatta
    27841 aattttttat ttattaagtt agtctatata tacccgcacc atataccttt aaacaatttt
    27901 tttaaaagtt ttttctaaat acttattttc cctgtgtcaa taatatgttt ctataataat
    27961 gtccctttaa ttttatcaat tctttatgat ttcctgattc cacaattcta ccattttcta
    28021 aaactattat attatcacaa tttgatatgg tacttaatct atgagctata attatacttg
    28081 tctttccata agttaaatta tttattgctt cttgaatttt agcttctgtt gctatatcta
    28141 tgttacttgt agcctcatcc aaaataagta ttggagcatt ttttaatact gctctagcaa
    28201 tacttattaa ttgtttttga ccttcactta aatcttcacc cctatccgat aatacagtac
    28261 tatatccttc acgcatttta actataaatt tatgtgcatt tgctatttta ctagcatata
    28321 taacttcttc atcagtagcc tccagatttc cataccttat attttccata actgtatcat
    28381 tgaatatata accttcttgt ggaactactc caaaacaagt tctcaaatag tttctgttaa
    28441 tagagtatat atccttacca tctaaaaata tacttccttc atctatatga taaaaatttg
    28501 taattagatt tataatagtt gtttttccac ttcccgtctc tcctatgatg gcatttgatg
    28561 tgccagcttt gatttttagg tttatattat ctaaaatttt cttttttgaa tcataagaaa
    28621 aacttaaatc tttaagctca atttctccct ttacaaaatg agagtttaaa ttaccatctt
    28681 tattatcgtt aatctcactt ttaaacatgt tatgcacttc ttgctcttct tgttcatcta
    28741 taatttcaaa aactctttca catccagcta tggctgaaag taatgtatta aatagatttg
    28801 ctaattcatt taaaggtctt gtaaattgtc ttacataact taaaaaacta gctataaccc
    28861 ctatggttat aatatcttta gatgcaagaa tgcctcctat aatacatata gttgcaaatc
    28921 ctatattact aatcacattc ataataggca ttaaaagaga agtgtaaatt tgcgatttaa
    28981 gggaaatatt tagaagtcta tcattgactt ctttaaaatc atttacagat tttctttcat
    29041 aattaaaaga ttttactaca tttatgtttg atatcatttc ttcaatatgt gcatttagtt
    29101 ttcccaactc aacttgttgt tgcttaaaaa aaactctagt tctctttgca ataaaattac
    29161 tcacaaacat tactatcggt attaatatta cactgcaaaa ggttaaaatt gggcttaata
    29221 caaacatcat aataaaagtc atcaatatgt ttaagattcc tgtaattaac tgtactaatg
    29281 aatttgatat tcctgtactg atattatcaa catcatttgt cactctactc ataatatctc
    29341 cagtagaact tttatcaaaa aaggttattg gtagattttt aaattttaaa aataaattac
    29401 ttctcatttc tttaactata ctttgagata atctagctgt acatatcccc ttagacagtt
    29461 taactgaaaa ctcaattata taaaatgaaa gtaaaataat tattcctttt aagaaaatgg
    29521 attttgaata agtatcgata tctattatgt caactacctt tccaatagta tatggtatta
    29581 cagttgatat aattgaatca attattacta gcaaaaaaat aaatctagta atcttcttat
    29641 cttttttaaa gtacataaaa attcttttaa acgttttttt aaaatcttta ggcttttctt
    29701 ttttctttaa tctactgacc ttctgatttc cagtacttaa attcaatata cttcctcctt
    29761 aatttacatc ttcacttact aaattttgca acgcaaaaat ttttttataa attggagaac
    29821 taaccagtaa atcttcatgt gtagcaaatc caaccacctc accctcatcc aagactaaaa
    29881 tcttatcaca gtcaattata gaactaatct tttgtgaaat agttattaca gtacatttct
    29941 tactttcctt ttctcttaaa ttacttatat actcttttat attctttctt ataattgtct
    30001 ctgtattaat atccattgca ctaaaacaat catcaagaat taatatttgt ggttttttgg
    30061 ctatagctct agctatagta attctttgtc tctgtcctcc tgataaattt ttaccccttt
    30121 ggaataaaac ttcatcataa gtattttcga gttttgttat aaattgattt gcatttgcta
    30181 tttcacatga ttttacaaca tcctctttgc atatatctct atttcccata actacattct
    30241 ccaatatact ttttccaaac acactactat tttgaaatac tacacctatt cttttcctta
    30301 aactttcttc agaaatactg tatatactac gtcctccaat cttaatatca cctttatcaa
    30361 ttatatataa tttttgaatt aaatttacta aagaagtctt tcctgaacca gtttgaccaa
    30421 tcacaccaat attttgacct tcttctattt taaaggatac gttttttaat attttttcat
    30481 ctgttttgtt atacgaaaaa tctacatttt caaaaattat atcacaattt acaaacttta
    30541 aatctttttc tccaacatct aaactttctt ctctctcaat tacttcttca ttctcatcaa
    30601 ccaaaacctc acttattctc tcataagatg cttttgccct aacaaaaaca ttaaaaacat
    30661 gtgaaatcat tataagagat gttaatattc tagtcatata gtttacataa gcaactataa
    30721 tcccaatttt aatttctcct gaataaatta aatgccttcc aatcagtaat attattatta
    30781 tggaaacatt aatgattatt gtaattgttg gtctaaaaaa tgacatcata gttgtagctt
    30841 ttctactatt tttaaaaagt tcatcattta actcttcaaa gctttggttc tcataatcgt
    30901 atctacaaaa tagtttaact acttttattc cacttaaaaa ctgtcttaat tttgaattta
    30961 ctttatctgt actttcttgt acatttttaa ataatctata ccctatcttc atattaagca
    31021 atattataat aaatataatt aaaactgcaa ttagaataat tattgacatc tttaaattta
    31081 attttatagc tataattaaa cttcctatac acattattgg tgactttata aaaactctca
    31141 tcattccatt aacgaattgt tgcatttgat tgatatcatt agtaattctt gttattaatg
    31201 aagctctacc aaatttatct atttgtttta atgagtatgt atttatcttt tcaaatacat
    31261 cctgtcttaa atcataagaa aaatgctgag aaactcttgc cgagattaca cttcttatta
    31321 ttgcaaatat tgcacctaaa aaagttatac ctatcattat agctcctgtt gatagtatgt
    31381 agtctaagtt cttatttgaa acccctatat ctattatttt tgccattata gttggttgag
    31441 ctaaatcaca aaaagcttcc aatgttaaaa aaaatactgc tgttagaaat aatactatat
    31501 atttttttat gtatttttta aaatacctca ttcaatcatc ccttcataga attttctcat
    31561 taccaaaagt atatcataat ggctaattta attgattata tcaattataa atatttgatt
    31621 tattattaat ttttatacca tatattttat aaattatttg tttttatttc c
//
LOCUS       DBGJFF010000031        31618 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.031, whole genome shotgun sequence.
ACCESSION   DBGJFF010000031 DBGJFF010000000
VERSION     DBGJFF010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 31618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31618
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.031"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            100..417
                     /gene="flgB"
                     /locus_tag="ACOIAF_001527"
     CDS             100..417
                     /gene="flgB"
                     /locus_tag="ACOIAF_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HGZ2668541.1"
                     /translation="MNTYDIIKMGLDATNLRSQTIANNIANINTEGYKRHYVTFEETL
                     NDAMPKIEEKTDNSKGLRVDGNNVDIEEEKVNQAMTTLQYNALISFANGKIAMTKSII
                     SGR"
     gene            425..829
                     /gene="flgC"
                     /locus_tag="ACOIAF_001528"
     CDS             425..829
                     /gene="flgC"
                     /locus_tag="ACOIAF_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HGZ2668542.1"
                     /translation="MSVFSGMRISASGLTAESLKMDIISSNVANINATRGPNGEAYRR
                     KVVSFEENYDNKLGMLGVKVTSVEDDKSPLKKVYDPSHPDAKPNGYVEYPNVNILVEM
                     TDLITASRAYDSNVDTLNAQKSMISKALEIGK"
     gene            839..1153
                     /gene="fliE"
                     /locus_tag="ACOIAF_001529"
     CDS             839..1153
                     /gene="fliE"
                     /locus_tag="ACOIAF_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HGZ2668543.1"
                     /translation="MPITQINNLMQGAKLDNLIGETKPLTLNKEEKKNFEDVIIDTID
                     KVNFQQVTADDMKEKFIKGEDVSMHEVMLIGQEAQLSMQYLIEVRNKIHDAYQEMNKV
                     QI"
     gene            1178..2728
                     /gene="fliF"
                     /locus_tag="ACOIAF_001530"
     CDS             1178..2728
                     /gene="fliF"
                     /locus_tag="ACOIAF_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HGZ2668544.1"
                     /translation="MIASFKNKIKEQVAKSRTKISSLKKGTKIALGIGVIAICLALVF
                     TAISASKNKYAVLFSELDVQDAADISAELESKKIDTKVKGSTIYVPRTEVDKLRLELA
                     PSLTSGSKGFALMDDSSSIGLTDEEFQIKKQRMIQGEIEKTIKSFPQVEDTRVHITFG
                     ESSVFEKEAVPGSAAVYLMLKPGTTLEKNQIKSVMALISGSVTNIPEKNVQVIDNKMN
                     LLSEGILEDENVNENNQPINSGISVDKNKASEQALNKQLEKSILEILEPIFGKGKVKA
                     TVNADLSFDTAEKTEIKIDPDKVAIKETRSKNSSKSATNNVQGVDANMNNQGNNNGTN
                     TEDSLDESFEYETGKIETHTIVAPGELNRLTASVAIDGKIAKGVQADVEDIVNNAIGM
                     DGARGDTLSVVSMVFDPEGKKEAKQELEEIKKEGFKKNIIKAVIGVVAVVAIGAIGYL
                     IYKKRSEREDMDYEDEDYISKSDSVDRKDNPLLGDDPELTLEEAVKMYAEEKPDQVTE
                     IIKTWLNE"
     gene            2741..3811
                     /gene="fliG"
                     /locus_tag="ACOIAF_001531"
     CDS             2741..3811
                     /gene="fliG"
                     /locus_tag="ACOIAF_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HGZ2668545.1"
                     /translation="MSTEITNVNAFDLSDINESDKKEKIEGEGLKKAAVLLMTLGPNV
                     SSDIIKSLPDKQVQKIGLEIANISSIASKERREILKEFVEINRAKDYVIEGGLEYARE
                     LLSNALGNAKANKLLEGISINTSTKPFKHIIKLDVKKILEILRNESAQTIAVLLAHME
                     PENGAEVLSYLDFDLQNEVAMRISSMGSISPEVIKVIESSLEDKLHSMSGDDFKDYGG
                     VSTLVQILINSDRKTEKNILGTIEEQDPDLAAQIKSHIFVFEDIVKLDDISIQKVLKE
                     VTMKDLVYALKGVDDEISDAIYRNQSSRAAEALREEMEMVGVIKVTQVEAAQQKIANI
                     VRKLEREGAITIVRNFGGDFVE"
     gene            3795..4535
                     /locus_tag="ACOIAF_001532"
     CDS             3795..4535
                     /locus_tag="ACOIAF_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HGZ2668546.1"
                     /translation="MTLLNNKIIKSKKALYKGVLDLTIIDKVRVGESNVNKENDCRFR
                     DEVIKEAIESAEIKKNEILEEAKMNAQNIEKQAYEKGYSQGQKNGYEDGYKESYDEYT
                     QKAKDEADFIKNQANLILLQANEKMTEYIKEKNKEIVRLAINMASSVLKKHFEDEESM
                     SDLLKQIIQDYEGSSSFIVRCNSFYKDNIEKEFELFKEETSLKSSVFVIADDSIDKGN
                     AIIQKENGRIIVGIDCAMEKLKEELLGE"
     gene            4539..5858
                     /gene="fliI"
                     /locus_tag="ACOIAF_001533"
     CDS             4539..5858
                     /gene="fliI"
                     /locus_tag="ACOIAF_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HGZ2668547.1"
                     /translation="MYKTDISIDFDKINKKIENLELTISEGRVKKIIGLTVEVEGIKA
                     FVGELCVIYNQVNKPVNCEVVGFKDKEVILMALGELTLIAPGCKVISKGIPLSVMCSD
                     NLLGKVLDGLGNPIDNSDAVLGDRYNLNNEPPDPMKRKKIRNIMETGVRAIDAFTTCG
                     EGQRIGIFAGSGVGKSTTLGMIARNAKADVNVIALIGERGREVLDFIDKDLGEEGMKK
                     SVVVCATSDKAPLVRLKGALTATAIAEYFRDQGKKVILMMDSVTRFAMAQREVGVAIG
                     EPPAQKGYTPSVFAMLPKLMERTGTSDKGSITAFYTVLVDGDDFNEPIADTTRGILDG
                     HIVLSRDLANKNHYPSIDVLNSLSRLMNEIASKEDIKIASFARDMLAEYREAEDLINI
                     GAYASGTNKKIDEAIYYHEHIINFLKQGINEKSSFNETISSLRRIFE"
     gene            5890..6336
                     /gene="fliJ"
                     /locus_tag="ACOIAF_001534"
     CDS             5890..6336
                     /gene="fliJ"
                     /locus_tag="ACOIAF_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012815984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HGZ2668548.1"
                     /translation="MELDKNFKFRLQKVLDLKIKDEEEIKMEFAKIQQKKIDIETNLE
                     NLESNYSKYSISKNNDNVQNQKITINYLLALNNSIMDLSEELDKSTNELEKARKQLIS
                     KQIERKSLEKLKEKKYGQYYKEEQLKEQSTNDEFASMSYLRNRQVL"
     gene            6364..7569
                     /locus_tag="ACOIAF_001535"
     CDS             6364..7569
                     /locus_tag="ACOIAF_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HGZ2668549.1"
                     /translation="MKIQADSYNIIKDIGNRKNSSKNKNIKNNKFEDNLYKTTDHKNV
                     KDRRETSKQNVSSDRPGNSDNECHLDEDKSNHLEDENTYEKDCSKNNEALQQLLNLLK
                     ENYKKDKDINTILNNIKKLSISDELKQELKLNKNILNKIANQNLELNGANTNNPNDIE
                     NLDIMKLRELLENNASEDIQVISKTTENDTKSIDLYSFDSNRMDTLNSNKQKDDSSDI
                     LEKLAGLNGNKSGNFGQVINKSVDMNKIKNDNVNLKQIESNVMDDSIKAIKHMKTNDI
                     QELTIKLRPKELGDMNIQLLKDGESMRAVVTVFNKDVFDSINKNIADLKQHLELTNVN
                     IKDVSVQMHSDNTNTSDTFDKAFEQQNRQNNQENMSKQNTNDKRNNVVIEDEIKENII
                     DDDRVDLLA"
     gene            7587..8195
                     /locus_tag="ACOIAF_001536"
     CDS             7587..8195
                     /locus_tag="ACOIAF_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HGZ2668550.1"
                     /translation="MYGIATALAGTQVNYRSNNLSQLERNINDIANKQVRSKGNQTVT
                     DNGTPIIGQGDDADKDLFMKILVAQMGNQDPMNPQDPTEYVSQLAQFATLEKMSAMND
                     KLDILLALSNSTLINSALSMATSLIGKEVEVSGEEDEDENIVGVVKSTYIKDGEVHLE
                     LEVDGSDKTVDVKYEKLLKIGEVEANSGEENDSSEQESPEEV"
     gene            8222..9205
                     /locus_tag="ACOIAF_001537"
     CDS             8222..9205
                     /locus_tag="ACOIAF_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HGZ2668551.1"
                     /translation="MIKAMYSGVSGMKANQTKLDVIGNNVANAGTTSFKKSSARITDS
                     FYQTVLYASAPTAALGGTNLGQVGVGSKISSINKDMTQGNVQPTNRPSDLMIDGDGYL
                     PVVRQGTVMYTRDGSFNLDTGFKAADGALKNIKGGRLVNGDGYMLQGMIYDGEYKADT
                     GEFVKKGDAKGKEPIVIPLQSISPKDGTVQNVLEYNISKDGTVEFLLSDGQRTQFVYD
                     IPDGAKVGDTPAGIDKLNGTIQKVQTYAFQNPAGLDAEGGNLFKPSANSGDPQIAGAT
                     GTIIQGAIEASNVDIAEEFTEMIIASRSFQANSKIISTSDEILQEIINLKR"
     gene            9218..9421
                     /locus_tag="ACOIAF_001538"
     CDS             9218..9421
                     /locus_tag="ACOIAF_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HGZ2668552.1"
                     /translation="MIRVTDLNGRKYILNSDLIVRIDSVPESRILLTTGEKNLVKESV
                     DEIVDKVIKYKRKINVGYIRSEK"
     gene            9421..10239
                     /locus_tag="ACOIAF_001539"
     CDS             9421..10239
                     /locus_tag="ACOIAF_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HGZ2668553.1"
                     /translation="MKKTDSLTFIGLILSTVLVLFGAVKGSSSGLKSFFDFASILITV
                     FGSFGALMITFTIDDIKLIKDALQYSFKTMNISKLDLLNQFKTLSKKARREGLLSIES
                     DVMKIQDPFMKKGLELCIDGLEINEIRSILELEIDSVEETNNRYSKLFKTWGTFAPAF
                     GMLGTLIGLIQMLSDLTSPDLIASGMGKALITTFYGSLLANIALNPIAYNIDEKTEKE
                     IYVKEMMLEGIISIQSGESSIVVEERLATYLSNNEKLEIMKSNKNTERAMSNGA"
     gene            10229..10924
                     /locus_tag="ACOIAF_001540"
     CDS             10229..10924
                     /locus_tag="ACOIAF_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HGZ2668554.1"
                     /translation="MARKKNKKKDEINPDAWLATYADTITLILTFFVLLYATSVVDME
                     KFKNIADALKGQFSGTSMFSTGSSTSEKSPIESVLVDKNDKSNLEQDLKDKVNNSNLQ
                     NSVTVKDDSRGILLELDDSILFDSGISELKEDSKATLNKVYDMIKMMKNKIVIEGHTD
                     NVPVKSSTHESNWDLSSSRAVSVVRYFVEEKGMDPKLFSATGCGEYQPLVSNETAKGR
                     AENRRVNILITID"
     gene            10936..11379
                     /gene="fliL"
                     /locus_tag="ACOIAF_001541"
     CDS             10936..11379
                     /gene="fliL"
                     /locus_tag="ACOIAF_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HGZ2668555.1"
                     /translation="MFKNKKIIIIILILIIFSAGAGFFILGEGGNKQIDKIAGLFIPT
                     GKGKDEVKTENLDLEEMVLKLADEGVRYLKVSITVSYDSSYKDIEKNTPLIRDNIINC
                     FMAKRADDFTADNLKNIKEEIKNTLNIQLGENVIQNIYFTNIVVQ"
     gene            11394..11768
                     /locus_tag="ACOIAF_001542"
     CDS             11394..11768
                     /locus_tag="ACOIAF_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliZ"
                     /protein_id="HGZ2668556.1"
                     /translation="MILYLLKILICIPIILILIVLSLKISQGTLLKKYNDKYIKILDI
                     VNISKNNSIIILKIGEEGCIMASSSSSMSKIKDLTKEEILRIENDIKERNFKIQDFKM
                     ERIKKKYDFSRYIGKFTKKEDK"
     gene            11769..12434
                     /gene="fliP"
                     /locus_tag="ACOIAF_001543"
     CDS             11769..12434
                     /gene="fliP"
                     /locus_tag="ACOIAF_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425215.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HGZ2668557.1"
                     /translation="MELLSKLPLADVPYTSSMQLFLFLTALMFLPFVMFMMTSFVRIV
                     ISLSFLKSALGAQQAIPSQILVGLAIVLTIFIMRPVLNEINEKALQPYMKEEVTMEEA
                     MKEAEGPIKEFLLKQTRQTDLDLFVEQAGLKEKKLTRENIPLSVVVPAFAISELKTAF
                     QIGFLIYIPFLIIDLVVASVLMSMGMFMLPPVMISLPFKLLLFVMVDGWNLIVKTLIL
                     GFG"
     gene            12447..12716
                     /gene="fliQ"
                     /locus_tag="ACOIAF_001544"
     CDS             12447..12716
                     /gene="fliQ"
                     /locus_tag="ACOIAF_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HGZ2668558.1"
                     /translation="MSEFMIMSVLKNGMFTGAMIAAPVLIVSLVVGLLISVFQATTQI
                     QEQTLTFVPKLIAILLVVAIGGSWMLHTLISFTSEIFNMMSLITK"
     gene            12728..14542
                     /locus_tag="ACOIAF_001545"
     CDS             12728..14542
                     /locus_tag="ACOIAF_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused FliR family export protein/FlhB family
                     type III secretion system protein"
                     /protein_id="HGZ2668559.1"
                     /translation="MIFVFIRIVAFFGTLRILFPAGTPALFKSLFAIIISILISSTMQ
                     IEYATNIDNIVLFTLYGINETITGIMLGYITNLCFYSIRMAGSMMDQQMGLSMINMFD
                     PNSMTQTTLINNLMNWTALMIFFSMDGHHVLIRGIRYSFELIPIGKSFIDNNIDYIIN
                     IFVQCFLTGFKIAIPIVLCLLMADFILGLISRSVPQLNVMIIGMPLKILVGIALFIIS
                     IPLIVNQISHLLSQIPKMYEGTFALAPMFFMGSTDKTEEATPKKKGEQRKKGNIAKSK
                     ELPIAMTLLAFTLLVPTLFSYVVNTLKSSLNYFLSLDFYMNINYSSIEKLVIAGLMDF
                     FKIFLPIAIPFLVLGIVANLFQVGILFTGETLKPNLSKLNPISGFKNMFSMRSLSTLI
                     KDTAIISILAYIGYTFFQDNYLDILKLGNIYLPTLMYTVKDLVYSILSKICVAMIVIA
                     VADYVYQRYSHKKQLRMTKQEVKDEYKNSEGDPEIKAKIKQKQRQISSQRTMQAVPSA
                     TVIVTNPTHLSIAVRYEKGKDQAPVVVAKGADYLAFKIREIAKGNDIPIIENKPIARL
                     LYKQVEIDQEIPEDMYQAFAEILVAVYKIKNRYKVPKR"
     gene            14551..16626
                     /gene="flhA"
                     /locus_tag="ACOIAF_001546"
     CDS             14551..16626
                     /gene="flhA"
                     /locus_tag="ACOIAF_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HGZ2668560.1"
                     /translation="MNKFNLKENMDVIVAFGVVGIVLMMIVPLPTFILDILLAINISL
                     SVLILLMTLFTTNVLDLSIFPTVLLVTTLFRLGLNLSSTRLILTQGYAGEVIEAFGSF
                     VVGGNYVVGIIIFLIILIVQFVVITNGSGRVSEVTARFTLDAMPGKQMSIDADLNAGV
                     IDDKTARKRRKELQQEADFYGSMDGANKFVKGDAIAGLIVTAINIIGGIVIGAVMLNM
                     TLMGAAQTYVRLTIGDGLVSQIPALLISTSAGIIVTRASTDENFGGLVGKQLTAIPKA
                     VMMTGCVLIVLGMMPGLPKPAFFSLGIGAIAAGYFLGREKKDTLEDLEFEKPDMVNPD
                     KNEVEDVTNLINVESIEVEIGYGLISLADESAGGDLLQRIASIRRQCAIDMGIVVQPI
                     RIRDNLQLNPNQYNIKIKGNVVGGYEIMPNMVLCMDPMNQGFSIFGIKTIEPTFGIEA
                     LWIESDKVEDAELKGYTVVDSSTILITHLLDIIKSNAHELLGRQEVKTIIDAARENYS
                     AVVDELIPDLMTLGEIQKVLKNLLREKVNIKDRVTILETLADQSRNTKDIELLTEYVR
                     IALARSICNNLVDEDKAIVVATLSPETEELVSNNLQRSVNGTYPAVDPENTNKIFESI
                     QEVMNNVYFNHNIPVIVVSPKIRAPFRKLVEIVYPNLTVLSLNEIPNDIKIKAEGVVS
                     IYDDEKVYS"
     gene            16604..17617
                     /gene="flhF"
                     /locus_tag="ACOIAF_001547"
     CDS             16604..17617
                     /gene="flhF"
                     /locus_tag="ACOIAF_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012815985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HGZ2668561.1"
                     /translation="MTKKYTANTIQDAMNLAKMELGDNITLIDKKEVRKSGIRGIFSK
                     KDIELTIGWEKKDNLEQKDLKREIEQLKSIINNMGFDNKNDNDIDKICKNLLNLDLNQ
                     EIVEFIKADLQEMKFNGIDTSKNLVESLKKKIKIENQAINGKIALVGPPGVGKTTTIA
                     KLAAKLVFEENKKVGVITIDTYRIGAVEQLKIYTDIMNIPFKGVISPDEMELALDEMK
                     DCDVVLIDTTGRGYKNSMQILEIKNLIDKAETDNIHLVLNCTTRESDTKAIIDSYRNV
                     NFKSLIITKLDETITYGSIFNIMNYAQKPISYITTGQNVPDDIIKPNEDKIIRLLLGV
                     ESI"
     gene            17614..18492
                     /locus_tag="ACOIAF_001548"
     CDS             17614..18492
                     /locus_tag="ACOIAF_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HGZ2668562.1"
                     /translation="MIDQAQILRKMAMEKRGSDEIIVENENMPKIITIASGKGGVGKS
                     NLATNLSICLTKLDKKVLILDADIGMSNIDIIMGVNVKGTIIDVINGEKNIEDIISQT
                     KYGVNIISGGSALNHIEDFTEAQRNKFIHSIEQIHNVDFIIIDTGAGMSKSLLSFIYC
                     STEFFLITTPEPTSLTDAYSLLKAISNFGIKKSANIIINRVIDLEEAKSTYKRIKMVV
                     DKFLNLNLELYGYIIDDKKVGVCVKKQIPFILEYPTSIASKCIIAIAKRMVSQSREDI
                     VDNKSFLRKMFSIFKS"
     gene            18506..19207
                     /locus_tag="ACOIAF_001549"
     CDS             18506..19207
                     /locus_tag="ACOIAF_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HGZ2668563.1"
                     /translation="MNREELIKENMPLVKSIARKFFIPGKGFEYQDLVNSGVIGLIDA
                     INKFDTEKGAKFSSYSYIKIKSAILDEIRNQSPISKHNLTKVNKYNRVVEKLQSELLR
                     DPTSYEIAKELNVSEKELHDIESNIDMLNIVSLNYVIFEDTNETVQDVISDREEEAPE
                     NVIEEEEKLEILSKAISNLNEREKLILSLYYYEDLNLKEIGKVLGVSESRVSQLHRKS
                     IRNLRNKIKELKYSI"
     gene            19223..19645
                     /locus_tag="ACOIAF_001550"
     CDS             19223..19645
                     /locus_tag="ACOIAF_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HGZ2668564.1"
                     /translation="MIQIILIVVSILIIVTIVINLHKESKNKDNRYFDKIFEQEYEAN
                     DRLIIAEKRRFFLDKVLAEIRSNNHIKVYENYNLIKANEERKENIFIESKKEVRQENE
                     LSLKVKHLISSGYDDVEICKMLDIGKGELSLIRSCYKI"
     gene            19856..20629
                     /locus_tag="ACOIAF_001551"
     CDS             19856..20629
                     /locus_tag="ACOIAF_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HGZ2668565.1"
                     /translation="MIRGLYTAVSAMITNQQKQNVVVNNLANMDTNGYKSKQLLTKSF
                     DELKLEGYNNYANGTKQKQIIGDISPGISMDETITNFNQGPIKTTDNKMDVAIQGKGF
                     FLVSDAAGNQFYTRNGNFRTDNKGDLITSEGYYVLGTNTATGAVGHINVGNQKFEVAK
                     DNTISLDGNAMYKFNIVDFNDYDNLKKEGDNLYSGGGAMKVNNALTIQSAIESSNVDM
                     VSEINNMMTISREYEANQKIIQAMDSKLAKIASEIGSVR"
     gene            20650..21411
                     /locus_tag="ACOIAF_001552"
     CDS             20650..21411
                     /locus_tag="ACOIAF_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HGZ2668566.1"
                     /translation="MYTMFYTSKTALSANQSKLDIISNNIANSDSTGYKKINMEYSDL
                     VDEVLNRPSYPTNGNDISTGTGAKATAPMRNYEQGALAPTDSKSNLAIDGEGFFRVIR
                     KDGTYAYTRNGGFNVDALGKIVDDNGNILDVQFDAGYNYNNTNIDSTNLTVSRNGELF
                     VDNKKIGKINLYQPIGTQNFISEGDSLFVATDAAQIKQVEKVDIVQGYRERSNVSLQE
                     EFVDLIATQRAFQMNSKGIKTADEMWQIANNLRAK"
     gene            21434..22315
                     /locus_tag="ACOIAF_001553"
     CDS             21434..22315
                     /locus_tag="ACOIAF_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HGZ2668567.1"
                     /translation="MNDMKEYDFGKPQSYSRENLTCLNFLLAEFCKKYKNYIIYELKC
                     NSSMVVDSIDQVNYQTFLDRVTSSCVVVQNAMEPVMKNFSFRIDRSVADMWIDITSGG
                     TGVVRDNDRELTELDKKVLLHLMDDLVRNMHLFEGFENIQVLDTHTHINLPQLCSPTA
                     PVCVVDLKVLNDNEYIGSVSLCFPYNGLEALLESISVIEFFDNDIGDDSEEFTKKIYN
                     SVSNIELSVIAEIGKVSINVEDLLKLEVGDVIVTNKKINDYIDIFVENSKSYTATPGF
                     ISSKKGVKINDAVGKEV"
     gene            22318..22692
                     /locus_tag="ACOIAF_001554"
     CDS             22318..22692
                     /locus_tag="ACOIAF_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HGZ2668568.1"
                     /translation="MDNSNLISDEEAKMLLNDKVESEVDGDDELALDVSEVNRNNIQR
                     ILDLKLELSVVIGRTKMTLRDILNLHKGSLIELDTLADQDVEILIDNKVLAYGKVVVV
                     DLNFGVKITSIVSEEDMVKSLV"
     gene            22847..24127
                     /locus_tag="ACOIAF_001555"
     CDS             22847..24127
                     /locus_tag="ACOIAF_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HGZ2668569.1"
                     /translation="MKFLKQARDLNPDDVDILNLTGLVNLLKCNFDEAVESFYKSSLC
                     EKTALCEKYINMLTSEEFLIFFKRYNQLIRLINRGMYKEAIQGFKLITEQYCDLIEPY
                     ELLALLYDKEEEYIKFDECLEVLKIIDKENYLLNQENNTLDMYNSANIQTKSNIKVDD
                     YKNEFLHKSSSNHKINDYNDVTKEKSSSKNQKSSDSNKKKSIKKNKYLYIAIILGILL
                     VGQSIYSSYKMNNLTDKISKNNETKNEESIDSNLTKKENNIEENEDMSKASNINKSNK
                     NINKEDDVKSKKNTVENEDDKDSDLFTEDELMSKANTLKSEKKTKSSIQLYKKVADIG
                     ENKGNTSEATYQVAILSEKLKDYKTAEEYYKMYVENYSEKDAYFDESYYNLGMMYYNN
                     GDLKNSKLTLKKLVNKVPNSMYNNSKVKEILKEE"
     gene            24243..26180
                     /gene="htpG"
                     /locus_tag="ACOIAF_001556"
     CDS             24243..26180
                     /gene="htpG"
                     /locus_tag="ACOIAF_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HGZ2668570.1"
                     /translation="MEFEKGSISIHTENIFPIIKKWLYSDKDIFIRELISNGCDAVSK
                     HKRLVSLGEISENKSSDYKITVSVNKGEGTLKFIDNGIGMTEEEIKKYINQVAFSGAE
                     DFFNKYKDKMEESNDIIGHFGLGFYSAFMVSKKVQIDTLSYAEGATPVRWISEGGTEY
                     EISESDARNDRGTTITLFIDDDSKEFLDEFTVRGIINKYCSFLPVEIYLEDVERLERE
                     AKEAEEKAKKQKEDGKEEIVDAKVIEPLNDTNPLWLKSPKDCTDEEYKEFYRKVFNVF
                     DEPLFWIHLNVDYPFNLKGILYFPKLKNEFELTEGKVKLYNNQVFVADNIKEVIPEFL
                     LLLKGVIDCPDLPLNVSRSFLQNDRDVSKISKHIIKKVADKLKSLCKNEREEYNKFWD
                     DIQIFIKYGCLKDESFYEKVKECILFKTIDDEYITLQDYLEKCKDKHENKVFYVSDKE
                     QQSQYIKLFKEYDLSAVVLNSSIDTHFISFMEYKENGVKFNRIDADLSDVLKDKNENK
                     DSEENKEEIAKIEGLFKEAVGERVKNYSVEGLKNEDTPAMVLVSEQSIRMAEMQSRFA
                     GMDLGMNFEEEKTLVINENSPIIKKLVSLKDDEEKKDKITLICNQIADLALLSNKELK
                     PDELDSFVQRSNKLVSLLIEL"
     gene            26568..27698
                     /locus_tag="ACOIAF_001557"
     CDS             26568..27698
                     /locus_tag="ACOIAF_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HGZ2668571.1"
                     /translation="MNFNYNLPVNILFGRGRINDVGKEVSKYGKKVLIVTGKNSTKKT
                     GLLDKTIDLLKDSKIEYEVFDRVEQNPLITTIYSGVEIIKSTGCDCVLGLGGGSIMDA
                     AKSIAFSYKNPGDLNDYIFGIRQGEEALPIILVPTTSGTGSEGNCFAVLTNSETKDKK
                     SIKKNSMYAKASIIDPELMVTMPKHIIASVGFDALAHNMEAYLSNGRNPLADVQAIYG
                     IELISENLIKVYNDVNDLEAWEKITLASTIGGMTIGTAGTALPHGMEHPVSGLRDVMH
                     GKGLAALTPTIIESSWDSDIERFGNISKVLGGTCAEDCSDAIRNFLKKIDLDIKLSDL
                     GIEKSDIDWLTDNCMKISVANIKRHPKYFNKEQIKEMYHKSL"
     gene            27925..28947
                     /gene="splB"
                     /locus_tag="ACOIAF_001558"
     CDS             27925..28947
                     /gene="splB"
                     /locus_tag="ACOIAF_001558"
                     /EC_number="4.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006290 - pyrimidine dimer repair [Evidence
                     IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore photoproduct lyase"
                     /protein_id="HGZ2668572.1"
                     /translation="MFNPKRIIFEKGTLDTDVGKKIYNKFRDNKDVEIINISSNRIKE
                     HIPGENLYEQYRSGKQTMVVGIRKSLKFQTCKPSANYQLPLVSGCMGRCEYCYLNTQL
                     GDKPFIRVFVNVDEILEQTKKYIEDRKPQVTIFEGAATSDPVPVEPYTNSLKKTIEFF
                     GREEKGRFRFVTKYSDVDSLLDAEHNNHTEIRFSINTPRIINEHEHYTASANKRIESA
                     VKLARAGYQIGFIIAPVFLYEGWKEEYKNLIISIKEALPKDFDKKIIFEVISHRYTTK
                     AKNRILEIYPDTLLPMENEDRKFKYGQFGYGKYVYDKEQLDDMKEFFLKEINGVFKNS
                     LIKYII"
     gene            29129..30040
                     /locus_tag="ACOIAF_001559"
     CDS             29129..30040
                     /locus_tag="ACOIAF_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HGZ2668573.1"
                     /translation="MHAWESIQITLDLIEANLSEEISINELANKANLSPFYYQRLFKR
                     LVNKTVMEYIKLRRLARASEYLAEHKNRILDVALNFGFASHETFTRSFKKAYGLTPEK
                     YRANPVKLNHFIKPELILNYAMVDEGVPLIADDIVIEVNRKTLSNPRTFVGIEIEVPI
                     CQLVGGETTGIAIVEELWMKLGSQRHNIPNQIPGGNEFAALYMGNAKEGNCMYMAGVE
                     VESGTSVEGYSTFELPAKEYLVCGFEAENFNELVNSAVFKADKFMERWMKKHNLTTTD
                     FAIEMYYPTTPEAAYLEHWIVPVPIEQ"
     gene            30055..30471
                     /locus_tag="ACOIAF_001560"
     CDS             30055..30471
                     /locus_tag="ACOIAF_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HGZ2668574.1"
                     /translation="MNWQDAFFQDTKPTFEQINEFINNPLWDKLNKTLVSTYNVEPKL
                     EYSKCSMQRGWNVKYKKRGKSLCTLYPQEGYFKALVIVSESNRVEVDLFINTCCDYIK
                     KIYNEVNFFNGSKWLMIQVDSLLVLNDMLELIKFRA"
     gene            complement(30571..30963)
                     /locus_tag="ACOIAF_001561"
     CDS             complement(30571..30963)
                     /locus_tag="ACOIAF_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HGZ2668575.1"
                     /translation="MSINTEKNITKNVEELTCPIRYALDIVGGKWKLPIICMLAVENP
                     IRYSSIKRKLDGITNTMLAQSLKDLESTGIVHRKQYNEIPPKVEYTLTSKGKSIVPIL
                     QQFANWGATNMQEKNTCGLSCKECRKIK"
     gene            31104..31499
                     /locus_tag="ACOIAF_001562"
     CDS             31104..31499
                     /locus_tag="ACOIAF_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HGZ2668576.1"
                     /translation="MKALEFLNKARVYYLATVDENNQAHVRPLGFVMEYNGKLTFCTS
                     NQKDMFKQLIFNPKVELCCIDQNLNTLRILGEAVFVTSKETQAKALEIMPSLGQMYSV
                     GDGKFEIFSIDKAKVSCCSMSGKKIDVEL"
BASE COUNT        12977 a         3360 c         5826 g         9455 t
ORIGIN      
        1 taatttaaat gaataaagaa gtcatttttt gtaatatagc aacttcattt ttttattaat
       61 aagcaaaata aataaaatat attttgagga ggagtgagtg tgaatacata tgacattata
      121 aaaatgggat tagatgcaac taatttaaga agtcagacaa tagcaaataa tatagcaaat
      181 ataaatactg aaggatataa gagacattat gtaacttttg aagaaacatt aaatgatgct
      241 atgcccaaaa tagaagaaaa aacagataat tctaaaggat taagagtgga tggcaataat
      301 gtagatatag aagaagaaaa agtaaaccaa gcaatgacaa cattacaata taatgctttg
      361 ataagttttg caaatggaaa aatagcaatg actaaatcaa taataagtgg gaggtaatta
      421 aaaggtgagt gtgttcagtg gaatgagaat atctgctagt ggattaacag cagaaagctt
      481 gaaaatggat atcatatcgt ccaatgtagc taatataaat gccacaagag gtccaaatgg
      541 agaagcatat aggagaaaag ttgtttcatt tgaagaaaac tatgataata aattaggtat
      601 gctaggagta aaagttacat ctgttgaaga tgataaatca cctcttaaaa aagtatatga
      661 cccatctcat ccagatgcaa agccaaatgg atatgtagaa tatccaaatg taaatatatt
      721 ggtagaaatg acagatttaa taacagcttc acgagcatat gattctaacg tagatacatt
      781 gaatgctcag aagtctatga tatcaaaagc tttggaaata ggaaagtagg gataaattat
      841 gccaattaca caaattaaca atttaatgca aggtgcaaag ttagataatt tgattggaga
      901 aacaaaacca ttaactctta ataaagaaga aaaaaagaat tttgaagatg taataataga
      961 tactatagat aaagttaatt ttcaacaagt gacagcagat gatatgaaag aaaaatttat
     1021 aaaaggcgaa gatgtttcta tgcatgaagt tatgttaata ggtcaagaag cacagctatc
     1081 tatgcaatat ttaatagaag ttagaaataa gattcatgat gcatatcaag aaatgaataa
     1141 ggtacaaatt tagccttttg aacaggagta ataatcaatg atagccagtt ttaaaaataa
     1201 gattaaagag caagttgcta aatctaggac aaagataagt tctctaaaaa agggaactaa
     1261 aatagcatta ggtatagggg ttattgctat atgtttagcg ctagtattta cagcaatatc
     1321 tgcgagcaaa aataagtatg cagtcttatt ttcagaacta gatgttcaag atgctgcaga
     1381 tatatcagca gaattagagt ctaaaaagat agatactaag gtaaagggaa gtacgattta
     1441 tgtacctaga acagaagttg ataagttaag gctggagtta gcaccaagtc ttacaagtgg
     1501 ttctaaagga tttgcactaa tggatgatag ttcatcaata ggacttactg atgaagagtt
     1561 tcagattaag aagcaaagaa tgatacaagg tgaaatagaa aaaacaataa agagttttcc
     1621 acaagtggaa gacactagag tacatataac atttggagag tcatcagtat ttgagaagga
     1681 ggctgttcca gggagtgcag cagtttattt gatgctaaaa ccagggacga ctttagaaaa
     1741 aaaccaaata aagtctgtta tggcacttat atcaggaagc gttacaaata taccagagaa
     1801 aaatgttcag gttattgata acaaaatgaa tttattatct gaaggtattt tggaggatga
     1861 aaatgttaat gaaaataatc aaccaataaa tagtggcatt agtgttgata aaaataaggc
     1921 atctgaacaa gcattgaata aacaactaga aaagtctatt ttagaaatac tagaaccaat
     1981 atttggtaag ggaaaggtga aagctacagt aaatgctgat ttaagttttg atacagctga
     2041 aaaaactgag ataaaaattg accctgataa agttgcaata aaagagacaa ggtcgaaaaa
     2101 ttcatctaaa tctgctacaa ataatgttca aggtgtagat gctaacatga ataaccaagg
     2161 aaataacaat ggtactaata cagaagatag tttagatgag agttttgagt atgaaacagg
     2221 taagattgaa actcatacta tagttgcgcc tggagaactt aatagattga cagcttctgt
     2281 tgcaatagat ggaaaaatag ctaaaggtgt tcaagctgat gtagaggata ttgttaataa
     2341 tgctataggt atggatggag cgaggggaga tactcttagc gttgtttcaa tggtatttga
     2401 cccagaaggt aagaaagagg ctaaacaaga gctggaagag attaaaaaag aaggatttaa
     2461 aaagaacata ataaaagcag ttattggagt agtggcagta gtagcaattg gagctattgg
     2521 atacttgata tataagaaaa gaagtgaacg agaagatatg gattatgaag atgaagatta
     2581 tatttctaaa tctgattctg ttgatagaaa agataatcct ctattaggag atgacccaga
     2641 actgacatta gaggaagctg ttaaaatgta tgcagaagaa aagccagacc aagtaacaga
     2701 gataataaaa acttggttaa atgagtaagg gggatagaaa gtgtccacgg aaattacaaa
     2761 tgttaatgcg tttgacttaa gtgatataaa tgagagtgat aagaaagaga agatagaagg
     2821 tgagggttta aaaaaagctg ctgtattatt aatgacatta ggaccaaatg tttctagtga
     2881 tataataaaa agcttgcctg ataaacaagt tcaaaaaata ggtttggaaa ttgcaaatat
     2941 ttcttcaata gcttcaaaag aaagaagaga aatcttaaaa gagtttgtag aaataaatag
     3001 ggcaaaagat tatgttatag aaggtgggct tgaatatgca agagagttgt taagcaatgc
     3061 tttaggaaat gccaaagcaa acaaattact tgaaggtata tcaataaata caagcacaaa
     3121 gccttttaaa catattataa aactagatgt aaagaaaata ttagaaatac ttaggaatga
     3181 aagtgcacaa actatagccg ttttacttgc acatatggag ccagaaaatg gagcagaagt
     3241 tttatcatac ttagattttg atttacaaaa tgaagtagct atgaggataa gctcaatggg
     3301 ttcaatatct ccagaagtaa taaaagtaat tgaaagttca ttagaagata aattacactc
     3361 aatgagtggt gatgatttta aggattatgg aggagtgtct accttagttc aaatattgat
     3421 taattctgat agaaaaacag aaaagaatat tttgggaact attgaagaac aagacccgga
     3481 tttagcagct caaattaaat ctcatatatt tgtctttgaa gatattgtca agttagatga
     3541 tataagtata caaaaagtgt taaaagaagt gactatgaaa gatttagtat atgccttaaa
     3601 aggtgtggat gatgagattt cagatgctat atatagaaat caatcttcta gggcagcaga
     3661 ggcacttaga gaagaaatgg aaatggttgg tgtcataaaa gttacacagg ttgaggctgc
     3721 tcaacaaaag atagcaaata tagtaagaaa acttgaaaga gaaggtgcca taactattgt
     3781 aaggaatttt ggtggtgact ttgttgaata ataagattat taaaagtaaa aaagcattat
     3841 ataaaggagt gctagattta accataatag ataaagttag agtgggagaa tcaaatgtaa
     3901 ataaagaaaa tgattgtaga tttagggatg aggtaataaa agaagctata gaatctgctg
     3961 agataaaaaa gaatgagata ttagaagaag caaaaatgaa tgcacaaaat atagaaaagc
     4021 aagcatatga aaaagggtat agtcaaggtc aaaaaaatgg atatgaggat ggatataaag
     4081 agtcatatga tgaatatacc caaaaagcta aagatgaagc agattttata aaaaatcagg
     4141 caaatttaat cttattacaa gctaatgaga agatgactga gtacataaaa gaaaaaaata
     4201 aagagattgt aagacttgct attaatatgg ccagttctgt tttaaaaaag catttcgaag
     4261 atgaagaatc tatgagtgat ttgttaaagc aaattattca agattatgaa ggaagtagtt
     4321 catttatagt aagatgtaat tctttctata aagataatat agagaaagag tttgaattat
     4381 tcaaagagga aacatcacta aaatcaagtg tatttgttat tgcagatgat agtatagata
     4441 aaggcaatgc tattatccaa aaggaaaatg gaagaataat agttggtatt gattgtgcta
     4501 tggaaaaact aaaagaagaa ttgcttggtg aataaagtat gtataaaact gatatatcta
     4561 tagattttga caaaataaat aagaaaatag aaaatttaga actcactata tcagaaggta
     4621 gagtaaagaa gataatagga cttactgtag aagttgaagg aataaaagct tttgttgggg
     4681 agttatgcgt tatatacaat caagttaata aaccagtgaa ttgtgaagta gtaggtttta
     4741 aagataaaga agtcatatta atggcgttgg gagaattaac attgatagca cctggttgta
     4801 aagttatttc taaggggata cctttaagtg ttatgtgtag tgataattta ctaggtaaag
     4861 tacttgatgg tcttggaaat cctatagaca actcagatgc tgttttagga gatagataca
     4921 acctgaacaa tgaacctcct gacccaatga agagaaaaaa gatacgaaat ataatggaaa
     4981 caggtgtaag agcaatagat gcatttacta cttgtggtga aggacagaga atcggcatat
     5041 ttgcaggaag tggagtaggt aagagtacga ctctaggtat gattgctagg aatgcaaaag
     5101 cagatgtaaa tgtaattgct cttattggag aaagaggaag agaagtacta gattttattg
     5161 ataaagattt aggtgaagag ggaatgaaaa aatctgttgt tgtgtgtgca acatcagata
     5221 aagcccctct agttaggtta aaaggtgctc ttactgctac tgcaatagca gaatatttta
     5281 gagaccaagg caaaaaagta atacttatga tggattcagt aactagattt gctatggcac
     5341 aaagagaagt tggtgtagca ataggagagc caccagctca aaaaggatat acaccatctg
     5401 tatttgcaat gttgccaaaa cttatggaga gaacagggac atcagataaa ggttctataa
     5461 ctgcatttta tacagtatta gttgatggag atgactttaa tgagccaata gcagatacaa
     5521 caagagggat acttgatggt catatagttt tatctagaga cttggcaaat aagaatcact
     5581 atccttctat agatgtatta aacagtttaa gtaggcttat gaatgaaatt gcttcaaaag
     5641 aagacataaa aatagcgtct tttgcaagag acatgcttgc tgagtataga gaagcagaag
     5701 atttgataaa tataggtgca tatgcttcag gtacaaataa aaaaatagat gaagcaatct
     5761 attatcatga gcatattatt aattttttaa aacaaggaat aaatgaaaag agttctttta
     5821 atgaaactat aagtagttta agaagaatct ttgaataaac aatttaaaaa aatatttgaa
     5881 taggttgtga tggaattgga taaaaatttt aagtttagat tacagaaagt tttagactta
     5941 aaaataaaag atgaagaaga gataaaaatg gaatttgcta agattcaaca aaaaaaaata
     6001 gatatagaaa ctaatttaga aaacttagaa agtaattaca gcaaatattc tataagtaaa
     6061 aataatgata atgttcaaaa tcaaaaaata actattaact atttgctagc tttaaacaac
     6121 tctataatgg atttatcaga agaactggat aagtcgacaa atgaacttga aaaagctaga
     6181 aaacagctta taagcaaaca gatagagaga aagtcattag aaaaattaaa agaaaaaaaa
     6241 tatggacagt attataaaga agaacagtta aaagaacaaa gcactaatga tgagtttgca
     6301 agcatgagtt atttgagaaa tagacaagtt ctataaaata ataggaagtg aggatattat
     6361 tgtatgaaga tacaagcaga ttcttataat attattaaag atattgggaa tagaaaaaac
     6421 tcatctaaga ataaaaatat aaaaaataat aaatttgaag ataatctata taagacaaca
     6481 gaccataaaa atgttaaaga tagaagagaa acaagtaagc aaaatgttag ttcagataga
     6541 ccgggaaata gtgataatga gtgtcattta gatgaagata aaagtaatca cttagaagat
     6601 gaaaatacat atgaaaaaga ttgtagtaag aataatgaag ctttacagca attactgaat
     6661 cttttaaaag agaattataa aaaagataaa gatataaaca ctatattgaa taatataaaa
     6721 aaactaagta tatcagatga attaaaacaa gaacttaagc ttaataaaaa tatattgaat
     6781 aaaattgcaa atcaaaattt agagttaaat ggagctaata caaataaccc taatgatata
     6841 gaaaatttag acataatgaa gttaagagaa cttttagaaa ataatgctag tgaagatatt
     6901 caagtaatta gtaaaactac agaaaatgat actaaaagca tagacttata cagttttgat
     6961 agtaatagaa tggatactct taactcaaat aaacagaaag atgatagttc tgatattttg
     7021 gaaaaactag cagggttaaa tggaaataag agtggaaatt ttggacaagt aataaataaa
     7081 agcgttgata tgaataaaat taaaaatgat aatgttaatt taaaacagat agaatctaat
     7141 gtaatggatg attctataaa agctattaag catatgaaaa caaatgatat acaagaattg
     7201 acaattaaat taagaccaaa agaattagga gatatgaaca tacaattatt aaaagatggt
     7261 gaaagtatga gagctgttgt gactgttttc aataaagatg tctttgactc tataaataaa
     7321 aacatagcag atttaaagca acatcttgaa cttacaaatg taaatataaa agatgtatca
     7381 gtgcaaatgc atagtgataa tacaaataca tctgatacct ttgataaagc atttgaacaa
     7441 cagaatagac aaaataacca agaaaacatg agtaagcaaa atacaaatga taagcgtaac
     7501 aatgtagtca tagaagatga gattaaagaa aatattattg atgatgatag agtagattta
     7561 ctagcttagt aagggggaag tattgcatgt acggaatagc aacggctttg gcaggaactc
     7621 aggtaaacta taggtcaaat aacttaagcc aacttgaaag aaatattaat gatattgcca
     7681 acaaacaagt tagaagtaaa ggaaatcaga cagtaacaga caatgggact ccaattatag
     7741 gacaaggtga tgatgctgat aaagacttgt ttatgaagat tcttgtagca cagatgggta
     7801 atcaagaccc aatgaatcca caagacccaa cggagtatgt atcacaatta gcacagtttg
     7861 caactttaga aaaaatgtca gctatgaatg ataagctaga tatactttta gccttaagta
     7921 actctactct aataaactct gctttgagta tggcaacatc attaattggt aaagaagtag
     7981 aagtatctgg ggaagaggat gaagatgaaa acattgtagg agtagtaaag tccacataca
     8041 taaaagatgg ggaagtacac ttagaattag aagtagatgg aagtgacaaa actgtagatg
     8101 ttaaatatga aaaattactt aaaataggag aggtagaggc aaattcagga gaagaaaatg
     8161 actcttcaga acaagaatct ccagaagaag tataatatta aatgggaaaa gaggtaaatt
     8221 tatgataaaa gcaatgtatt caggtgtaag tggaatgaaa gcaaatcaaa caaaactaga
     8281 tgtaatcggt aacaatgtag ccaacgctgg aactacttct tttaagaaaa gttcagctag
     8341 aataacagat tcattttacc aaactgtact atatgcaagt gctccaacag ctgcactagg
     8401 aggaacaaac ttaggacaag taggtgtagg tagtaagata tcaagtataa acaaagatat
     8461 gacacaagga aatgtacaac caactaatag accatctgat ttaatgatag atggagatgg
     8521 atatcttcca gtagtaagac aaggaacagt tatgtatacg agagatggtt cttttaattt
     8581 agatacagga tttaaagcag ctgatggtgc tttgaagaat attaaaggtg gaagacttgt
     8641 aaatggtgat ggatatatgt tacaaggtat gatttatgat ggggaatata aagctgatac
     8701 tggagaattt gttaaaaagg gtgatgctaa aggaaaagag cctattgtaa taccacttca
     8761 aagtataagt cctaaggatg gaacagttca aaatgtacta gagtataata tatcaaagga
     8821 tggaacagta gaatttttat tgagtgatgg tcaaagaact caatttgtct atgatatacc
     8881 tgatggtgct aaggtaggag atacacctgc tggaatagat aaattaaacg gtacaataca
     8941 aaaagtgcaa acatatgcat tccaaaatcc agcaggacta gatgcagaag gaggaaactt
     9001 gttcaagcca tcagcaaact caggagaccc acaaatagca ggagctacag gtacaataat
     9061 acaaggagct atagaagctt ccaatgtaga tatagcagaa gaatttacag aaatgataat
     9121 agcaagtagg tctttccaag caaacagtaa aataatatcg acatcagacg aaatacttca
     9181 agaaataata aatcttaaga gatagtaggt acatattatg ataagagtaa cggatttaaa
     9241 cggaagaaaa tacatattaa attcagatct tatagtgaga attgactccg tgccagaaag
     9301 caggattctc cttacaacag gagaaaaaaa tcttgtaaaa gagtcagtag atgagatagt
     9361 agataaagta ataaagtata aaagaaaaat aaatgtaggt tatattagga gtgaaaaata
     9421 atgaaaaaaa cagattcact aacattcatt ggactaatat tatctacagt attagtttta
     9481 tttggagcag taaaaggaag tagttcagga ttgaaaagtt ttttcgattt tgcatctata
     9541 ttaatcacag tatttgggtc atttggagct ttgatgatta catttactat tgacgatata
     9601 aaacttataa aagatgctct acaatactca ttcaagacaa tgaatatatc gaaattagac
     9661 ttattaaatc aatttaaaac actttctaaa aaagcaagaa gagaaggact tttatctata
     9721 gaaagtgatg taatgaaaat acaagaccca tttatgaaaa aaggattaga gttatgtatt
     9781 gatggtcttg aaataaatga aataaggtct atactagagc tcgaaataga ttctgttgaa
     9841 gaaaccaata acagatattc taaactattt aaaacttggg ggactttcgc acctgcattt
     9901 ggtatgttgg gaactctaat aggtttaata caaatgttat ctgaccttac atctccagat
     9961 ttaatagcat ctggtatggg aaaagcatta attacgacat tttatgggtc tttacttgcc
    10021 aatatcgctt tgaacccaat tgcatataat atagatgaga agactgaaaa agaaatttat
    10081 gtaaaggaaa tgatgctgga ggggataata agcatacaat ctggagagag cagcatagta
    10141 gttgaggaac gtttagctac atacctttca aataatgaga agttagaaat tatgaaatcc
    10201 aataaaaata cagaaagagc aatgagtaat ggcgcgtaaa aaaaataaga aaaaggatga
    10261 gataaatcca gatgcatggt tggctactta tgcagatacg ataacactga ttcttacatt
    10321 ttttgtgtta ttgtatgcta catcagttgt agatatggaa aaatttaaaa atatagcaga
    10381 tgcgttaaaa ggtcagtttt ctgggacaag tatgtttagt acaggttcaa gtacaagtga
    10441 gaaatctcca atagagagtg ttttagtgga taaaaatgat aagtcaaatt tagaacagga
    10501 tttaaaagat aaagttaata attctaactt acaaaatagt gtaacagtca aagatgatag
    10561 tagagggatt ttactagagc tagatgattc aattttattt gactctggga tttctgaatt
    10621 aaaagaagat agtaaagcca ctcttaataa agtatacgat atgataaaga tgatgaaaaa
    10681 taagatagtt atagaaggcc atactgataa tgtacctgta aaatcaagta cgcatgaatc
    10741 caattgggat ttatcttcta gtagagcagt aagtgttgta agatactttg tagaagaaaa
    10801 gggcatggac cctaaactat tttcggcaac aggatgtgga gagtaccaac ctttggtatc
    10861 aaatgaaaca gcaaagggaa gagctgagaa tagaagagtt aatatactta taactattga
    10921 ctaggaggaa gaggtttgtt caaaaataaa aaaattatta taattatttt gatactgatt
    10981 attttctcag caggagcagg tttttttata ttaggagaag gtggaaataa acaaattgac
    11041 aagattgcag gtttgtttat tccaacaggg aaaggcaaag atgaagtaaa gaccgaaaat
    11101 cttgacttag aagagatggt attgaagcta gctgatgaag gtgtaagata tttaaaagta
    11161 tcaataactg tatcttatga ttcttcatat aaagatatag aaaaaaatac gcctttaatt
    11221 agggataata ttataaattg ttttatggca aaaagggcag atgattttac tgctgataat
    11281 ttaaagaaca taaaagaaga aattaaaaat acattaaata tacaactagg tgaaaatgta
    11341 attcaaaata tatatttcac aaatatagta gtgcaataaa aggatggtaa gacatgatac
    11401 tttatttact aaaaatatta atatgtatcc caataatttt aatattgata gtcttatctt
    11461 taaaaattag ccaaggaact ttattaaaaa aatataatga taaatatata aaaatactag
    11521 atattgtaaa tataagtaaa aacaatagca taataatttt aaaaataggt gaagaaggat
    11581 gcattatggc atcttcttct tctagcatga gtaaaataaa agatttgacc aaagaagaaa
    11641 tattaagaat agaaaacgac attaaagaaa gaaattttaa aattcaagat tttaaaatgg
    11701 aaagaattaa aaagaaatat gatttcagta gatatattgg aaaatttaca aaaaaggaag
    11761 ataagtaaat ggaactatta tcaaaattac ctttggctga tgtaccatat acaagttcaa
    11821 tgcaattgtt tttatttcta actgcattaa tgtttttacc atttgtaatg ttcatgatga
    11881 caagctttgt cagaatagtt ataagtttat catttttgaa atcagcatta ggagcacagc
    11941 aagcaattcc aagtcaaatt ttggtagggc ttgcaatagt attgacaata tttataatga
    12001 gaccagtatt aaatgagata aacgaaaagg cattacaacc atatatgaaa gaagaagtta
    12061 ctatggaaga agctatgaag gaagcagaag gacctattaa agaattttta ttaaagcaaa
    12121 ccagacagac agatttagat ttatttgtag agcaagctgg tttaaaagaa aagaaattaa
    12181 ctcgtgaaaa tataccatta tctgttgtag ttccagcatt tgctataagt gaactaaaaa
    12241 ctgcttttca aataggtttt ttaatatata taccattttt gataatagac cttgttgtgg
    12301 caagtgtttt gatgtctatg ggtatgttta tgttacctcc agttatgata tcattaccat
    12361 ttaaattatt attatttgta atggtggatg gatggaactt gatagtaaaa acattgatat
    12421 tgggatttgg ataggagaaa aatagtatga gtgaatttat gataatgagt gttcttaaaa
    12481 atggcatgtt tacaggagct atgattgcag ctccagtgct aattgtttct cttgtagtgg
    12541 ggcttttaat aagtgtattt caagccacaa ctcaaatcca agagcaaact ttgacttttg
    12601 taccaaaact aatagcaatt cttttagtag tagctattgg tggaagctgg atgttacata
    12661 ctcttattag ctttacttcg gagatattca atatgatgtc tctcataact aaataagtag
    12721 gatatatatg atttttgtat ttattagaat agtagcattc tttggaacac taaggatact
    12781 atttccagct ggtacacctg ctttatttaa gtcgctcttt gcaatcatta tatcaatctt
    12841 gatttcaagt actatgcaaa ttgaatatgc aacaaatata gacaacattg tattgtttac
    12901 cttatatggt ataaatgaga ctataactgg gattatgctt ggatatataa ccaatctatg
    12961 tttttatagt atcagaatgg ctggttctat gatggaccaa cagatgggat tatcaatgat
    13021 taatatgttt gacccaaatt caatgactca gacaacttta atcaataatc ttatgaactg
    13081 gacagcactt atgatatttt ttagcatgga tggtcatcat gttttaatta gaggcataag
    13141 gtatagtttt gagttaattc caatagggaa atcattcata gataataata tagattatat
    13201 aataaacata tttgtgcagt gctttttaac tggattcaaa atagcaatac ctattgtact
    13261 ttgtctgctt atggcagatt ttatattagg gcttatatca agaagtgtac ctcaactaaa
    13321 tgtaatgata attggtatgc cattaaaaat actggttggt attgcattat ttatcatatc
    13381 aataccttta attgtcaatc aaataagtca tttattaagt caaataccaa agatgtatga
    13441 gggaactttt gcactagctc ctatgttttt tatgggttca acagataaaa cagaagaagc
    13501 tactccaaag aaaaagggag aacaaagaaa aaaaggtaat atagcaaaga gtaaagaatt
    13561 acccattgca atgactctac ttgcattcac tttattagta cctacattat tttcatatgt
    13621 agtgaataca cttaaatctt ctcttaatta ttttttgagt ctagattttt atatgaacat
    13681 aaattattca agtatagaga aattagttat agcaggatta atggattttt ttaagatatt
    13741 tttaccaata gctattccat tcttagtttt aggtatagtc gcaaacctat ttcaagtagg
    13801 aatactgttt actggtgaaa ctttaaaacc caatttatca aaattaaatc caattagtgg
    13861 attcaaaaac atgttttcta tgagaagttt gtcaacctta ataaaagata cagcgattat
    13921 atctattttg gcttacatag gatatacatt cttccaagat aattatttag atatattaaa
    13981 gctaggtaac atatacctgc caactttaat gtacacagtt aaagatttag tatattcaat
    14041 attaagtaag atttgtgtag ctatgatagt tatagcagtt gcagattatg tttatcaaag
    14101 atacagtcat aaaaagcaac ttagaatgac aaaacaagaa gttaaagatg aatataaaaa
    14161 ctcagaagga gacccagaaa taaaagctaa gataaaacaa aaacaaagac aaatttcgtc
    14221 tcaaagaacc atgcaagcag taccaagtgc tactgtaata gtaaccaatc ccacccactt
    14281 atctatagca gtgagatatg aaaaagggaa agaccaagct ccagtagtgg tggcaaaagg
    14341 tgcagattat ttagctttta aaataaggga aattgcaaag ggaaatgaca tacctataat
    14401 agaaaataaa cctattgcta ggttgttata taagcaagta gaaatagacc aagaaattcc
    14461 agaggacatg tatcaagctt ttgcagagat attagtagca gtatataaaa tcaaaaatag
    14521 atacaaagtc cccaaaaggt aggataagta atgaataagt ttaatttaaa ggaaaatatg
    14581 gacgtaatag tggcatttgg tgttgtaggg attgttctta tgatgatagt gccgttacct
    14641 acatttatat tagatatttt actggcaatt aacataagtt tatctgtgct aattttacta
    14701 atgacactat ttacaacaaa tgtacttgat ttatctatat ttccaacagt gttacttgtg
    14761 acaacactat ttagattggg attaaatctt tcatcaacta gattgatttt aacacaggga
    14821 tatgcaggtg aagttataga ggcatttgga agttttgttg taggtggaaa ttacgtagta
    14881 ggaataatta tattcttaat tatattaatt gttcaatttg ttgttataac caatggttct
    14941 ggaagagtat ctgaagtaac agctagattt acgttagatg ccatgccagg taagcagatg
    15001 agtatagatg cagatttaaa tgcaggagtt atagatgaca aaactgctag aaaaagaaga
    15061 aaagagcttc agcaagaagc agatttttat ggctctatgg atggagcaaa taagtttgta
    15121 aagggagatg ccatagcagg tcttatagta acagcaataa acataatagg tggaattgta
    15181 ataggagctg taatgctgaa tatgactctt atgggtgcag cacagacata tgtaagactt
    15241 actatagggg atggtcttgt aagccagata cctgctctat taatatctac atcagcaggt
    15301 ataatcgtaa caagagcttc tactgatgaa aactttggtg gattagtagg taaacagtta
    15361 acagcaatac caaaagcagt aatgatgact ggctgtgttc taatcgtact tggtatgatg
    15421 cctggattac ctaaaccagc atttttttca ctaggtatag gtgctatagc agcaggttac
    15481 tttttaggaa gagaaaagaa agatacttta gaagacttgg agtttgaaaa acctgatatg
    15541 gtaaatccag ataaaaatga agtagaagat gtaacaaact taataaatgt agaatcaata
    15601 gaagtagaaa ttggatatgg tttaatatct ttagctgatg aatcagcagg aggagattta
    15661 cttcagagaa tagcatcaat aagaagacaa tgtgcaatag acatgggtat agtcgtacaa
    15721 cctataagaa taagagataa tttacagtta aatccaaacc aatacaatat aaaaataaaa
    15781 gggaatgtag ttggtgggta tgagataatg cctaatatgg ttttatgtat ggatcctatg
    15841 aatcaaggat ttagtatatt tggaataaag acaattgagc ctacttttgg aatagaggca
    15901 ctttggatag aaagtgacaa ggtagaagat gcagagctaa agggttatac agttgtagat
    15961 tcatccacaa tacttataac tcacttacta gatataataa aatcaaatgc acacgaatta
    16021 ctgggaagac aagaagttaa gactattata gatgcagcaa gagaaaatta tagtgctgta
    16081 gtagatgaat tgataccaga tttaatgaca cttggtgaaa ttcaaaaagt acttaaaaat
    16141 ctgttaagag aaaaagtaaa tataaaggat agagttacaa tacttgagac acttgcagac
    16201 cagtctagaa atacaaaaga tatagagctt cttactgaat atgtaagaat agctttggct
    16261 agaagtattt gtaataattt agttgatgaa gataaggcaa ttgtagtggc tacattgtct
    16321 ccagaaactg aagaattggt atctaataat ttacaaaggt ctgtaaatgg aacttatcca
    16381 gcagttgacc cagaaaatac aaataaaata tttgagagta tacaagaagt aatgaacaat
    16441 gtatatttca atcataatat accagtaata gtagtatcac caaagataag agctccattt
    16501 agaaaattag ttgaaattgt atatcctaat ttgactgtat tatcattaaa tgaaatacct
    16561 aacgatatta aaatcaaagc agaaggtgtg gtgagcatat atgatgacga aaaagtatac
    16621 agctaacaca attcaagatg ctatgaatct agccaaaatg gaattgggtg acaatattac
    16681 attaatagat aaaaaagagg taagaaaatc tggaataaga ggcatttttt caaagaaaga
    16741 tattgaattg actattggat gggaaaagaa agataattta gaacaaaaag atttaaaacg
    16801 agagatagaa caactaaagt caattataaa taatatgggg tttgataata aaaatgataa
    16861 tgatatagat aaaatatgca agaatttact aaacttagat ttaaatcaag aaattgtaga
    16921 atttataaaa gctgatttac aagaaatgaa gttcaatgga attgatacaa gtaaaaattt
    16981 agtagaaagt ctaaagaaaa agattaaaat agaaaatcaa gcaataaatg gtaaaattgc
    17041 tttagttgga ccaccaggag taggtaaaac gactacaata gcaaagttgg ctgccaaatt
    17101 agtttttgag gaaaataaaa aagtaggagt aataactata gatacataca ggattggtgc
    17161 tgtagaacag ttaaagatat atactgatat aatgaatatt ccttttaaag gggtaataag
    17221 ccctgatgag atggaattgg cacttgatga gatgaaagat tgtgatgttg tattaataga
    17281 tacaacaggt agaggctata aaaactctat gcaaatatta gaaatcaaaa atcttataga
    17341 taaagctgaa actgataata tacatttagt tttgaactgt acaacaagag aaagtgatac
    17401 aaaagcaatt atagattcat atagaaatgt gaattttaaa agcttaataa ttacaaagtt
    17461 agatgaaaca ataacatatg gctcaatatt taacataatg aattatgcac aaaaaccaat
    17521 atcatatatt acaacaggac aaaatgttcc tgatgatatt ataaaaccta atgaagataa
    17581 gattataaga ttactattag gagttgaaag tatatgatag accaagctca aattttgaga
    17641 aaaatggcaa tggaaaaaag aggttctgat gagattatag tagagaatga gaatatgcct
    17701 aagataatta caatagcttc tggtaaaggc ggcgttggaa agagtaatct cgctacaaat
    17761 ttgtctatat gcttaaccaa acttgataaa aaagttctta tattagatgc agatatagga
    17821 atgtctaata tagatataat aatgggggta aatgtaaaag ggactataat tgatgtaatt
    17881 aatggcgaaa aaaatataga agatataatt tcacagacta agtatggtgt aaatataata
    17941 tctggaggtt ctgcccttaa tcatatagaa gactttacag aagctcaaag aaataagttt
    18001 atacatagca tagaacagat tcacaatgtg gattttataa taattgatac aggagctggt
    18061 atgagcaaaa gcctattgtc atttatatac tgtagtactg aatttttctt aataacaact
    18121 ccagaaccga catcattgac tgacgcatat agtttattaa aagctataag taattttggc
    18181 ataaagaaaa gtgcaaatat aataatcaat agggtaattg atttagaaga agcgaaatct
    18241 acatacaaaa gaataaaaat ggttgtggac aaatttctaa accttaatct ggaattgtat
    18301 ggatatataa tagatgacaa aaaagtaggt gtatgtgtaa aaaaacagat accattcatt
    18361 ttagaatatc ctacaagtat tgcatcaaaa tgtattatag ctatagcaaa gagaatggta
    18421 agtcagtcaa gagaagatat tgttgataat aaaagttttc taagaaagat gtttagtata
    18481 tttaaaagct aaggaggcgt agttaatgaa tagagaagaa ttaataaaag agaatatgcc
    18541 tcttgtaaag agtatagcaa gaaaattttt tataccaggg aaaggttttg aatatcaaga
    18601 cttagtaaat agtggagtca ttggattgat tgatgcaata aataaatttg atactgaaaa
    18661 aggagcaaag ttttctagtt actcttatat aaagataaaa tctgctatat tagatgaaat
    18721 tagaaatcaa agtccaattt ctaaacataa tctaactaaa gtaaataagt ataatagagt
    18781 ggtagaaaag ttgcaatctg aattattaag agatccaact tcatatgaaa ttgcaaaaga
    18841 attaaatgtt tcagaaaaag aacttcatga tattgaaagt aatatagaca tgctaaatat
    18901 tgtatcatta aattatgtaa tctttgaaga tacaaatgaa actgtacaag atgtaataag
    18961 tgatagagaa gaggaagctc cagaaaatgt aatagaagaa gaagaaaagt tagagattct
    19021 atccaaagca attagtaatt taaatgaaag agaaaaatta atactttcac tgtactatta
    19081 tgaagattta aatctaaagg agatagggaa agtgctaggt gtttcagaat caagagtttc
    19141 acaactccac agaaaatcaa taagaaactt aagaaacaag ataaaagaac ttaaatattc
    19201 tatatagatg gtgattgata agatgataca aattatatta atagtagtgt ctattttaat
    19261 aatagtaact atagttataa atttacataa ggaatcaaaa aataaagata acagatactt
    19321 tgataaaata tttgagcaag aatatgaagc aaatgataga ttaataattg cagaaaaaag
    19381 aagattcttt ttagacaaag tacttgctga aataagaagt aataatcata taaaagttta
    19441 tgaaaattat aacctaataa aggctaatga agaaagaaag gaaaacatat ttatagaatc
    19501 aaaaaaggaa gtaagacaag aaaatgagtt atctcttaaa gtgaaacatc ttataagttc
    19561 aggttatgat gatgtggaaa tatgtaagat gctagatatt ggaaaggggg aactgtcatt
    19621 aataaggagt tgttacaaaa tataaggata ttattatgtg ataaaaaagt attcttagga
    19681 ataggtattg gtatattaat atcaacttta tgtataatgc tttttagtaa taatgatatg
    19741 agtaaggcag agatagaaaa aaaggccaga gcgttaggca tgaaatatac aagtgagatg
    19801 aaagtcttag aatagatgat actgtttaga catttacaaa aaaggaggtc ataatgtgat
    19861 aagaggatta tatacagcag tttctgctat gataactaat caacaaaagc aaaatgttgt
    19921 tgtcaataac ctagctaata tggatacaaa tggatataaa agtaaacaac ttttgactaa
    19981 aagttttgat gaattaaagt tggaaggtta caataactac gcaaatggaa ctaaacaaaa
    20041 gcagattata ggcgatataa gtccaggtat ttctatggat gaaactatca caaattttaa
    20101 tcaaggacct ataaaaacta ctgataataa aatggatgta gctattcaag gtaagggatt
    20161 ttttctagtt agtgatgcag ctggaaatca attttacact agaaatggga acttcagaac
    20221 agacaataaa ggtgacctta taacaagtga agggtattat gtacttggga cgaatactgc
    20281 tactggtgct gtaggtcata taaatgtagg aaatcaaaaa tttgaagtgg caaaagataa
    20341 tacaatatca ctagacggaa atgctatgta taaattcaat attgtagatt ttaatgacta
    20401 cgataactta aaaaaagaag gagataattt gtattctgga ggaggagcta tgaaagtaaa
    20461 taatgcactt acaatacaaa gtgcgataga gtcctctaat gtagatatgg tatctgaaat
    20521 taataatatg atgacaatat caagagaata tgaggcaaat caaaagataa ttcaggctat
    20581 ggattctaaa ttagcaaaaa tagcaagtga gattggttca gtaagatagg taataggagg
    20641 tgaattttta tgtacacaat gttttatact agtaaaacag ctttatcagc aaatcaaagt
    20701 aaactagata taatatctaa caatatagca aattctgatt caacaggata taaaaaaata
    20761 aatatggaat attctgattt agtggatgaa gtactaaata gaccatcata tcctacaaat
    20821 ggcaatgata tatcgactgg aacaggagct aaggcaacag ctcccatgag aaattatgaa
    20881 caaggtgcat tggctcctac agatagtaag agtaatttgg caatagatgg agaaggattc
    20941 tttagagtga taagaaaaga tggtacatat gcatatacaa gaaatggtgg atttaatgta
    21001 gatgccttag gtaagattgt agatgataat ggaaatatat tagatgtcca atttgatgca
    21061 ggatataact ataataatac aaatatagat agtacaaacc tgacagtttc aagaaatgga
    21121 gagctattcg tagataataa aaaaatagga aaaatcaact tatatcaacc tataggaact
    21181 caaaatttta tatctgaagg agatagttta ttcgtcgcaa ctgatgcagc acagattaaa
    21241 caagttgaga aagtcgatat agttcaaggc tatagagaga ggtcaaatgt ttctttacaa
    21301 gaagaatttg ttgacttaat agccactcaa agagcatttc aaatgaactc aaagggaata
    21361 aagacagcag atgaaatgtg gcaaatagcg aataatttaa gagctaaata attatgtaag
    21421 gtgaggaata ttcatgaatg atatgaaaga gtacgatttt ggcaaacctc agagttattc
    21481 aagagaaaat ttaacttgtt taaatttttt attagctgaa ttttgtaaaa aatataaaaa
    21541 ctatataatt tatgaactta agtgtaattc tagtatggtt gttgatagta tagaccaagt
    21601 aaattatcag acttttttag atagagtaac ttcttcatgc gtagtagtac aaaatgcaat
    21661 ggaacctgtt atgaaaaact tttcttttag aatagataga agtgtagctg atatgtggat
    21721 tgatataact tctggaggaa ctggagttgt aagagataat gatagagagc ttacagaact
    21781 tgataaaaaa gtgcttttac accttatgga tgatttagta agaaatatgc acttatttga
    21841 gggttttgaa aatatacaag tattggatac acatacacat attaatttac cacaattatg
    21901 ttctccaact gcaccagtat gcgttgtaga tttaaaagtg ctaaatgata atgagtatat
    21961 tggaagtgtt tctctttgtt ttccatacaa tggtttagaa gctctgttag aaagtatatc
    22021 tgttatagaa ttttttgata atgatatagg agatgattca gaggaattta ccaaaaaaat
    22081 atataatagt gtatctaata tagaattatc ggttattgca gagataggaa aagtatcaat
    22141 taatgttgaa gacttactta aactagaagt tggagatgta atagtaacaa ataaaaaaat
    22201 aaatgattat atagatatat ttgtagaaaa ttcaaaatca tatacagcaa caccaggatt
    22261 tatatcaagt aaaaaaggtg taaaaataaa tgatgctgtt ggaaaagagg tgtagttatg
    22321 gacaatagta atcttatttc agatgaagaa gcaaagatgc tattaaatga taaagttgaa
    22381 agtgaagttg atggagatga tgagttagcc ttagatgtgt ctgaagttaa tcgaaataat
    22441 atacaaagga tacttgacct taagttagaa ttatctgtgg ttataggaag aactaagatg
    22501 actctaagag atattttaaa cctacataaa ggttctctta ttgaactgga tactttagca
    22561 gaccaagatg tggaaatact aatagataat aaagtgttgg cttatggaaa agtagtggtg
    22621 gtagatttaa attttggtgt aaagataaca agtatagtaa gtgaagaaga tatggtaaaa
    22681 tctcttgttt agagataaca tcaattaata cttaaggcat gaggtggtac ttattttaaa
    22741 cttaaatgat ataaaaaaag atatattaaa actcaataaa aaagactcat gttctctata
    22801 taatgaatct attaataata taaataataa ttgtatatcg agtggattga aatttttaaa
    22861 acaagcaaga gatttaaacc cagatgatgt tgatatatta aatctcacag gtcttgtaaa
    22921 tttactaaaa tgtaattttg atgaggctgt agaaagtttc tataagagtt cactttgtga
    22981 aaaaactgct ctgtgtgaaa agtatataaa catgcttaca tctgaggagt ttttaatttt
    23041 ctttaaaagg tacaatcaac tcataaggtt aataaacaga ggaatgtata aagaagctat
    23101 acaagggttt aaacttataa ctgagcaata ctgtgattta atagaacctt atgaattgct
    23161 tgccttactg tatgacaagg aagaagaata tataaaattt gatgaatgtt tagaagtatt
    23221 gaagattatt gataaggaaa attatttatt aaatcaagaa aataacacat tagatatgta
    23281 taatagtgct aatatacaaa ctaaaagtaa tataaaagta gatgattata agaatgagtt
    23341 cttacataaa agtagtagta accataaaat taatgattat aatgatgtaa ctaaagaaaa
    23401 gagttcaagc aaaaaccaga aaagttcaga ttctaataag aaaaaaagta ttaaaaaaaa
    23461 taaataccta tatatagcca taatattagg aatattatta gtaggacaaa gtatatatag
    23521 ttcttataag atgaataatt taactgataa aatatctaaa aataatgaaa ccaaaaatga
    23581 agagtcaata gattctaact tgactaagaa agaaaataat atagaagaaa atgaagatat
    23641 gagtaaggct tctaatataa ataagagtaa taaaaacatt aataaagagg atgatgtaaa
    23701 atctaaaaaa aatactgtag aaaatgaaga tgataaagat tctgacttat ttacagagga
    23761 tgaattgatg tctaaggcga atactttaaa atcagaaaag aaaactaaat cttctataca
    23821 actatataag aaagttgcag atataggtga aaataaaggg aatacatctg aagcaactta
    23881 tcaagttgct attctaagtg aaaagttaaa agactataaa actgctgaag aatattataa
    23941 aatgtatgta gaaaattact cagaaaaaga tgcttatttt gatgaatctt attataattt
    24001 aggtatgatg tactataaca atggtgattt aaaaaattct aaattgacat taaagaagtt
    24061 ggttaataag gtaccaaata gtatgtataa taattcaaaa gtaaaagaaa ttttaaaaga
    24121 agaatagtta aattagataa gaaaaataaa ttattttatt ttaaaataat atgattataa
    24181 tatattgaca taccatttta aaaaatttaa aatattaatt ataaaatgag gagggataaa
    24241 aaatggaatt tgaaaaaggg agtatttcaa ttcatacaga aaatattttt cctataatta
    24301 aaaaatggtt atactctgat aaagatatat ttataagaga gcttataagt aatggatgtg
    24361 atgcagtaag taaacataaa agacttgtat cactaggaga aatatcagaa aataagtcat
    24421 cagattataa aattacagta tctgtaaata aaggagaagg tacacttaaa tttattgaca
    24481 atggaattgg tatgactgaa gaagagataa aaaaatacat aaatcaagtt gctttttcag
    24541 gtgctgaaga tttctttaat aaatataagg ataaaatgga agagtcaaat gatataatag
    24601 gtcattttgg attaggattt tattctgcat ttatggtgtc taaaaaggta caaatagata
    24661 cactttcata tgcagaagga gcgactcctg taagatggat atctgaaggt ggtacagaat
    24721 acgaaatatc tgaatcagat gctagaaatg atagaggaac tactataaca ctatttattg
    24781 atgatgatag taaagaattt ttagatgaat ttactgtaag aggaataata aataaatact
    24841 gttctttctt accagtagaa atatatttag aagatgttga aagattagaa agagaagcta
    24901 aagaagcaga agaaaaagct aaaaaacaaa aggaagatgg aaaagaagag attgtagatg
    24961 caaaagtaat tgagccttta aacgacacta atcctctatg gttaaaatct ccaaaagact
    25021 gtactgatga ggagtataaa gagttttata gaaaggtatt caatgtattt gatgaacctt
    25081 tattttggat acatttaaat gtagattatc ctttcaattt aaagggtata ctttacttcc
    25141 ctaaattaaa aaatgagttt gaactgactg aaggaaaagt aaaattatac aacaatcaag
    25201 tgtttgtggc tgacaatata aaagaagtta taccagaatt tttactttta ttaaaaggag
    25261 ttatagactg cccagactta ccattaaatg tatcaagaag tttcttacaa aatgacagag
    25321 acgtaagcaa aatttctaag catataatta aaaaagtagc tgataagtta aaatctcttt
    25381 gtaaaaatga aagagaagaa tacaataagt tttgggatga tatacaaata tttattaaat
    25441 atggttgctt aaaagatgaa agtttctatg agaaggtaaa agaatgtata ttattcaaaa
    25501 ctattgatga tgagtatata acattacaag attatttaga aaaatgtaag gacaaacatg
    25561 aaaataaggt attttatgta agtgataagg aacagcaatc tcaatacatt aaactattta
    25621 aagaatacga cttaagtgct gttgttttaa actcatcaat agatactcat tttatttcat
    25681 ttatggaata taaagagaat ggagttaagt ttaatagaat agatgcagac ctttctgatg
    25741 tattaaaaga taaaaatgaa aataaagata gtgaagaaaa caaagaagag atagctaaga
    25801 tagaaggttt gtttaaagaa gctgtcggag aaagagtgaa gaattattct gtagaaggat
    25861 tgaaaaatga agacactcca gctatggtat tagtatcaga acaatctatt agaatggcag
    25921 aaatgcagtc aagatttgct ggaatggatt taggaatgaa ttttgaggaa gaaaaaactc
    25981 ttgtaataaa tgagaacagt ccaataatca aaaaattggt ttctcttaaa gatgatgagg
    26041 aaaagaaaga taagattacg cttatttgta accagatagc agatttagct ttactttcta
    26101 ataaagaatt aaagccagat gagttagatt catttgtaca aagaagtaat aagcttgtga
    26161 gtcttttgat agaattataa gattgaattt gaatacattg aagcaaattt ataaaaagta
    26221 ttcaatatca gtgatgataa agaagtgtct caaaatgaac ttttcagttc atggggcgct
    26281 ttttttatgt gaaattaaat ttattttaat catttcaaca atgaaaaata tatttatttc
    26341 tattttgaga cagaccattc tttatttttt tatctttggt taatttttaa atatttaagt
    26401 aggttctaat aaaaaactct atttagttaa atatagttaa cttgatatat aataagataa
    26461 tacatataat taagtaaaag attgtttttt agtttatgtt taaatacata attacacttt
    26521 gttaaaaaca taaaattatt actaaagtag aaataggagg ttttagcatg aattttaatt
    26581 ataatttgcc agtaaatatc ttatttggga gaggcagaat taatgatgtg ggaaaagaag
    26641 tatctaaata tggcaaaaaa gtattgatag taacaggtaa aaatagtaca aagaaaactg
    26701 gattacttga taaaacaata gatttactaa aagactctaa aatagaatat gaagtttttg
    26761 atagagtaga acaaaatcct cttataacta ctatatattc aggagtagaa attataaaat
    26821 caactggatg tgattgtgtt ttagggcttg gtggtggaag tattatggat gctgccaaga
    26881 gtatagcttt ttcttataaa aatccaggag atttaaatga ttatatattt ggaattagac
    26941 aaggtgaaga agctttacct ataatcttag ttccaacaac atcaggtaca ggtagtgaag
    27001 gaaattgttt tgcagtactt acaaattcag aaactaaaga taaaaaatct ataaagaaga
    27061 atagtatgta tgcaaaagca tcaataattg acccagaact tatggttaca atgccaaaac
    27121 atataattgc atcagttgga tttgatgcac ttgcccataa tatggaagca tatctttcta
    27181 atggaagaaa ccctcttgca gatgtacaag ctatttatgg gattgaactt atatcagaaa
    27241 acctaataaa ggtatataat gatgttaatg atttagaagc atgggaaaag ataacacttg
    27301 caagtactat tggaggaatg acgattggta cagcgggtac agccttgcca catggaatgg
    27361 agcatcctgt aagtggtctt agagatgtaa tgcatggaaa aggacttgca gctttgactc
    27421 ctactattat agaaagttca tgggatagtg atattgaaag atttggtaat atttcaaagg
    27481 tattaggagg aacttgtgca gaagattgtt cagatgcaat tagaaatttc cttaaaaaaa
    27541 tagatttaga tataaagcta agtgatttag gtattgaaaa atcagatata gattggttaa
    27601 ctgataattg tatgaaaata tcagttgcaa atattaagag acatccaaag tattttaata
    27661 aggaacaaat aaaggaaatg taccataagt ctttataatt tgactttaca tatttagttt
    27721 tatataaaaa ttataaatta ataataaaaa attataagta attaatagta aaaaaattgt
    27781 gtatagaagt tatatataca ataaacttgt cttaagcttt tattttactt tctaataaat
    27841 aaaggagttt aaaaaaggga agattaatta agtaatattt tataaaattc ttcgatttaa
    27901 aaactttaag tagaggtgaa ataaatgttt aatcccaaaa gaataatatt cgagaaagga
    27961 actttagaca ctgatgtggg taaaaaaata tacaataaat ttagagataa taaagatgta
    28021 gagataatta atatatcatc aaataggatt aaagaacata ttccaggtga aaatctatat
    28081 gaacaatata gaagtggtaa gcagactatg gtagttggga ttagaaaaag tctgaagttt
    28141 caaacatgta aaccttctgc taattatcaa ctaccacttg tcagtggatg tatgggaaga
    28201 tgtgagtatt gttatttgaa cacacaatta ggtgataaac catttataag ggtgtttgta
    28261 aatgtagatg aaatacttga acagacaaaa aaatatattg aagatagaaa gccacaggta
    28321 accatatttg aaggggcagc tacatcagac ccagttccag tagagccata tactaattct
    28381 ttaaagaaaa caatagagtt ttttggaaga gaggaaaaag gaagatttag atttgttact
    28441 aaatacagtg atgtggatag cttattggat gctgaacata ataatcatac agaaatacga
    28501 tttagtatca atactcctag aattataaat gaacatgagc attatacagc ctctgcaaat
    28561 aaaagaatag agtcagctgt aaaacttgct agggctggtt atcagattgg tttcataata
    28621 gcacctgtgt ttttatatga aggttggaaa gaagagtata aaaatctgat tataagtata
    28681 aaagaagctt taccaaagga ttttgataaa aaaattatat ttgaagttat atctcataga
    28741 tatacaacta aagcaaaaaa tagaatacta gagatttatc cagatacact attgccaatg
    28801 gaaaatgagg atagaaagtt taaatacggt caatttggat atggtaagta cgtgtatgat
    28861 aaagaacaat tagatgatat gaaagagttc tttcttaagg aaataaatgg agtatttaaa
    28921 aatagtttaa taaaatatat aatatagcaa atcttaccta aagatttgct atattttatt
    28981 tttatacatt ttatgtttat aataaagcaa tgagatttag cactatcata taagaaaatt
    29041 aaaaagggca ctatctatca agaacatttt ttctagtttt gatactataa aggataagag
    29101 taacaaaaat atcaggagga gttatagtat gcatgcatgg gagtctattc aaattacatt
    29161 ggatttaata gaagcaaatt tatcagaaga aatatcaatt aatgaattag caaataaagc
    29221 aaatttatca ccattttact atcaaagact atttaaaaga ttggtaaata agactgtaat
    29281 ggagtatata aagcttcgtc gtcttgcaag agcttctgaa tacttagcag aacataagaa
    29341 tcgtatactt gatgtagctt taaattttgg atttgctagt catgagacat ttacacgttc
    29401 ctttaaaaaa gcatatggat taactccaga aaaatataga gcaaatccag taaaactcaa
    29461 tcactttatt aagccagaat taattttaaa ttatgctatg gttgatgaag gtgttccact
    29521 gattgcagat gatatagtga ttgaagtcaa tcgtaagaca ttgagtaatc caaggacatt
    29581 tgttggaata gaaatagaag tacctatttg ccagttggtg ggcggtgaaa caactggaat
    29641 tgcaatagta gaggaattat ggatgaaact tggaagtcaa agacataata taccaaatca
    29701 aattccaggt ggaaatgaat ttgctgctct ttatatggga aatgcaaaag aaggaaattg
    29761 tatgtatatg gcaggtgtag aggtagagtc aggtacatca gtagaaggat atagtacttt
    29821 tgagttgcca gctaaagaat atttagtatg tggatttgag gcagaaaact ttaatgagtt
    29881 agttaattca gctgttttta aagcagataa atttatggaa agatggatga agaaacataa
    29941 tttaacaact actgattttg caattgagat gtattacccc actacacctg aagcagcata
    30001 tctggaacat tggattgttc cggttcctat tgaacaatag aaaggatgat gaatatgaat
    30061 tggcaagatg cgttttttca agatactaaa cctacatttg agcaaattaa tgaatttatt
    30121 aataatccat tatgggacaa gttaaataaa actttggttt ctacttataa tgtagaacct
    30181 aagcttgaat acagtaaatg ttcaatgcaa agaggttgga atgtaaaata taaaaaaaga
    30241 ggtaaatcac tatgtacttt atatccacaa gaaggttatt ttaaagcttt ggttattgta
    30301 agtgaaagca atagagtaga ggttgattta tttattaata cttgttgtga ttatatcaaa
    30361 aaaatatata atgaggtaaa cttttttaat ggaagtaaat ggctaatgat tcaagtagat
    30421 agtttattgg tattaaacga tatgcttgaa ctaattaaat ttagagctta acatatagaa
    30481 caggatgaat atataagtca tcctgttttt atattcaatc agataaatta atatatagtc
    30541 tataatatga ataatttagt aaatttaaat ttactttatc tttctacatt ctttgcaact
    30601 aagtccacat gtgttttttt cttgcatatt agtagcaccc caattagcaa attgttgtaa
    30661 aataggcaca atacttttac ctttacttgt taatgtatat tcaacttttg gaggaatttc
    30721 attatattgt ttacgatgaa caataccagt agactctaaa tcttttaatg attgagctag
    30781 catagtattt gtaatgccat ccaattttct ttttatgcta ctatatctaa ttggattttc
    30841 aactgctaac atacatataa taggtaattt ccacttacca ccgacaatgt ctaatgcata
    30901 acggattgga caggttaatt cttcaacgtt tttagttata tttttttcag tatttatact
    30961 cattttaatt actccttagt tatagtagta tcttttttat actttatcag aaaaaactgc
    31021 atacttgttt tttctgtatt ttatattaaa attatatcaa aggataatcc ttaatacaag
    31081 tatttaaaag gggtgtaaga attatgaaag cattagaatt tttgaacaag gcacgagttt
    31141 attatttagc aacagttgat gagaacaatc aagcacatgt gcgtccttta ggatttgtta
    31201 tggaatacaa tggtaaactt acattttgca catctaatca aaaggatatg tttaagcaac
    31261 ttatttttaa tccaaaggtt gaactttgtt gtatagacca aaaccttaat actctacgta
    31321 ttttaggtga agcagtattt gttacatcaa aagaaacaca agctaaggca cttgaaataa
    31381 tgccaagttt aggtcagatg tactctgtag gagatggtaa gtttgaaata ttctctattg
    31441 ataaggcaaa agttagttgt tgttcaatga gtggtaaaaa gattgatgta gagctttaat
    31501 tataattgaa tcgacatcca tagtgaaaat taagaaaacg ttattactga aatgttgata
    31561 gatttaattt aaatgcaata aaaagatttt taaataatga atatgtaaaa atttttag
//
LOCUS       DBGJFF010000032        31276 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.032, whole genome shotgun sequence.
ACCESSION   DBGJFF010000032 DBGJFF010000000
VERSION     DBGJFF010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 31276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31276
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.032"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(95..1123)
                     /locus_tag="ACOIAF_001563"
     CDS             complement(95..1123)
                     /locus_tag="ACOIAF_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HGZ2668577.1"
                     /translation="MKRVYIDFEMNMPNSKSKRAVFNSDIIAIGAVMYDEKTKNIDKF
                     KSLIRPVSNEELYPHIQELTHISSEELKSAPSYEEVMRKFKKWLGIFSDIKGIYTFGN
                     LDLTCFNNTDMRSAKKNNHPRFVNNIRDLFVDIKEKYINCGMRCMNYISLKNLLEFVN
                     LEFSGDAHDPLNDAYNLFILDEAITNNVDIQNLLIIRDIIRPPFNDINSNLDNCFNKF
                     KESLYKKEGNYNIVDFSVEIIKTVRMYLLTIIDVNIQNLEIMKDISKKMDTIDKLKDI
                     EEGYFYLLEDVYFDMKDVLEDLMLYRMNEDEYKYEIKNIIKMFDEDLDNEKIYINKCN
                     NLNVVNKI"
     gene            complement(1171..2031)
                     /locus_tag="ACOIAF_001564"
     CDS             complement(1171..2031)
                     /locus_tag="ACOIAF_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HGZ2668578.1"
                     /translation="MSELSGKGCEIIVPFEERLPVRDIEKSIIKKYRKNLWSKFMKAI
                     RDYKLVEEGDKIAVAISGGKDSILMAKMFQELKKHGQVNFDVEFIAMDPGYHANIRQL
                     LIDNCEYLNIPIHLFDSRIFEIADEIAKDYPCYMCARMRRGALYSKAEELGCNKLALG
                     HHYDDVIETTMLNLLCAGNFKTMLPKLNSTNFEGIKIIRPLYYIREEHIIRFIQNSGI
                     WPLNCACMVAAKKTGNKRYEIKDLIKSLESNFKNVEKSIFKAAENVNLDSVLGWQKDG
                     EKHSFLENFE"
     gene            2300..2509
                     /locus_tag="ACOIAF_001565"
     CDS             2300..2509
                     /locus_tag="ACOIAF_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HGZ2668579.1"
                     /translation="MENKTRDIKKNCGPSLARPYINNQIFTEMYCLSDALKFGTIFPE
                     LNLLESEMYNKELYAKPKKYRGGRR"
     gene            2509..2775
                     /locus_tag="ACOIAF_001566"
     CDS             2509..2775
                     /locus_tag="ACOIAF_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HGZ2668580.1"
                     /translation="MKDSSRAELMRKVQETSFACVDMNLYLDNHPDDKNAINTYNSLC
                     NQFAQARYAYENKYGPLTNFGYAPSRCPWQWADQPWPWDREFNY"
     gene            2804..3376
                     /locus_tag="ACOIAF_001567"
     CDS             2804..3376
                     /locus_tag="ACOIAF_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HGZ2668581.1"
                     /translation="MWIYQKTIQHPVNIKTCDPRMAKFLITQFGGPNGELAASLRYLS
                     QRYTMPTGNMRALLTDIGTEELAHVELICTMVYQLTSDASPEELKAAGLGSNYAQNGY
                     GIYPTDSNGVPFDVRPIAVMSNPVTDLHEDMAAEQKALATYYQLINLTDDVDVIDVLK
                     FLGQREIIHYQRFGEALMDAYELEESQKMF"
     gene            complement(3611..4801)
                     /locus_tag="ACOIAF_001568"
     CDS             complement(3611..4801)
                     /locus_tag="ACOIAF_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HGZ2668582.1"
                     /translation="MNFNQKRIAASIMATAIIMPTMGNLAYANESEVESVSIESRTIT
                     GNAVNFRKGPGTNHESMGKLYKGDKVEYVGKEGSWVKVKYNGNTGYVHGNYVAINSLG
                     SSNESSDTSVKSTKVVTAKGLNFRTGPSTSSSKISTLGYGTEVGYISESNGWSKISSN
                     GRVGYVSSKYLGTSVNDSTNENAENSSNDLVKGTKVVTAKSLNVRTGPGTSHSKIATL
                     SYGTEVGSISESGGWTKVSYGNQTGYVSSQYLAEKGSVDTSIPSYSTNSPSQGADSVI
                     SFAKTLLGKPYVWGAEGPNSFDCSGFTQYVMKKSVGVSIPRVSRDQSKYGTYVNRGDL
                     RSGDLVFFDTQGSNNGSVSHVGIYIGNGDMIHASSGSSKKVTISNINSSYYSSRYVNA
                     RRVL"
     gene            complement(5061..5492)
                     /gene="dut"
                     /locus_tag="ACOIAF_001569"
     CDS             complement(5061..5492)
                     /gene="dut"
                     /locus_tag="ACOIAF_001569"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HGZ2668583.1"
                     /translation="MYNLKVKKLNDDAIIPNFAHKGDAGMDLYSIEEVVIPPGETKLI
                     KTGICIELPTMTEAQVRPRSGLALKHSVTVLNTPGTIDEGYRGELKIILINHGKNDFK
                     VEKHMKIAQMIVKPIYDINIEEVKELSDSERGKGGFGSTGF"
     gene            5605..7833
                     /locus_tag="ACOIAF_001570"
     CDS             5605..7833
                     /locus_tag="ACOIAF_001570"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HGZ2668584.1"
                     /translation="MKLVLAEKPSVAKTIASFLGAKTRQDGYFEGNDYIITYAVGHLV
                     SLYDMKDYDKDKYSGSWKMNNFPFVPEDKFKFKIDSSKTKQFNTVKKLLNRKDVEYVI
                     NATDNDREGELIAFLIFLLAKNKKPVKRILVNEWTPEDITRGIKNLKDEDEMRNLQAA
                     GYTRLITDWLIGINFTSVATLKYGNGKLLNIGRVILPTVKLVYDRDMEILNFVPKTYY
                     EIEGHFKAEAGEYKGKYVKGKESKFDTLEDANKIIASITSETGKILDKKVTMSKEYAP
                     KLFSLTSLQGYITSKYSNFTSDKVLNVCQSLYEGNGKGGYITYPRTDSIFLEESLASK
                     ASQTLDKLKVGLEYENKIKFSKTKRVFDSSKVDSHSAIIPTYIIPKSLSKDEQLVYDA
                     IKDRFVANFMPPAEYENTEIKTEVDNCTFLTKGKVLKSKGYLEVYNKEEKNDLLPLVN
                     KDDVVDVLEIKPLTKQTTPPKPYTEDTLLKAMKNCGKNVPEEDTTVLSGYSIGTSATR
                     ADVLKKISQVGYVKKKGKSYSITELGKNLVEIFPVKDLFDVDYTGKLEKSLSDIQKGK
                     YTRKEYLTNIISFIYQNVNIIKQDASKNINTEAYTFDSKTKKFTKTKEKKQKKDVTNT
                     EKEVAISKDSNNKKEVKNGDENQSLGKCPICQGDVLEFDKGFACKNHKECKFVIWKND
                     KYLSLYKKKVNKTMVKNILKKGETKVKSLTAKNGNKFDATLKYDKNPDTGYFNWKIEF
                     DN"
     gene            complement(7944..8429)
                     /locus_tag="ACOIAF_001571"
     CDS             complement(7944..8429)
                     /locus_tag="ACOIAF_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HGZ2668585.1"
                     /translation="MDNNNILKIADSFLDFLFVLENNIFKESDLLKKFQNNSDIMKAY
                     FEECPMAPSHAKVILYLMTSNSSSISQIASNLGILKSNMTPIIDRLVEHGLVNKFPDP
                     KDRRILRVELTDKAFKLFDAVKAILKESLVKKLSNLSEEELTLLDEHTLKLSEIVKKL
                     G"
     gene            8681..9904
                     /locus_tag="ACOIAF_001572"
     CDS             8681..9904
                     /locus_tag="ACOIAF_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HGZ2668586.1"
                     /translation="MKRYLIVFLMCSSLFLVGCGKTEESEPEKPIAVSVQKAIGGEIE
                     NTNSFSGTTKVKDETAVTAQTVGTVQEVYVKLGQNVRKGDELLSISSPELENSVKQSK
                     ASLDLAKASYSSATGGSLEAQVNQAKTALDNAKIQYEESQRNYDNNKILYEQEVISLD
                     QFKKIEFSLEQTKQQLDSAQRAYDTATSKSIPQAKALAKKQLDQAQVSYNLAMSNLDK
                     LTLTSPVDGTITAKNFDSKEMITQSQPAFIISNPNILEVDLNVAESDIGKFKKDGNVD
                     VIIEDQRILGKIDYVPSVVDPQTSLYPVKVLVNNTNNKFKAGMSAQVNLSVEKENGAV
                     TVPKKAIFEENGKKYVYIATKDNIAKKHLVQTGIVTEDKIEIKSGVSDKDTVIIGGIS
                     LISDGTKIFPVEKEK"
     gene            9908..13042
                     /locus_tag="ACOIAF_001573"
     CDS             9908..13042
                     /locus_tag="ACOIAF_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HGZ2668587.1"
                     /translation="MNLTQTSVKRPLTIIMVFLVLIVFGGIGYKKMSINLMPDIEIPV
                     VMVMTTWTGAGPQDVDEQVSQKVDESLSAVSNVKSTISSSQESVSMVVAQFEFGTNLD
                     EIMNDVRSKVDALQTSLPDDAAKPTVLKLDMNAQAIGQLVISGGNENSSQALRKYAED
                     VIQPKIESIDGVTSADLKGGKKAQVNVIADPAVLSNYGVSLSTIKGVLSSSNKTFPYG
                     SITQGEDKIVLRAIDKLESLDDIKQIQIPVKGGNTVRLDNLCSVDYGYADKDSIYRYN
                     GKDSLVIDISKQQDANTVKVMQSAYKYVDELNKENPQFKIKIANDTSSYISESINSVM
                     SNLLISAVIAFIVIFAFLKSVRASLVVAVAIPISIIGAIAILYFTGESLNLITASSLV
                     ISVGMVVDNATVVIENIFKYRKSGRLSLDDCAIEGTRTVTNAILASTLTTIAIFLPIM
                     FTEGIAKITFGALGKTLIASLAFSFIGAITLVPSVFAKLSRGKNSQKMQEKDSPIFDK
                     VSEAYKKLLSVCLRHQRLVVISSLLIFVASLFGMGFIGMDFMPAADKGELSISIELPK
                     GLSLESNDYYVSMTEKKASDIPEIKTLITTLTSDSNNSKASIAIELESQKNRKRTTDE
                     IEKEIIDRVATVPDCKINVSQNSSVMGGAGGADFTLEMKGPDLDVLKILGKEAEKEFS
                     SIKGFRNVETSLTDTTQEAQFTIDKHKAQEYGVNTAEIAGMLRTAINGDSVTTATIDD
                     YKVDVNLKFKADTIDSLEDIKQLKVNSATGKEVPLGAFSDIKMADGLQSISRTDGDFS
                     VNITATLDNLDAGTATKLALEKTNKMVIPKDYSIGVGGETEMMNESMASLGFSMVIAI
                     ALVYMVMVAQFESFSKPFIIMFCVPFAFVGVVLSLLVSGQSLSTVGMLGIILLIGIVV
                     NNGIVLIDYIEQLRKTNRNNDLIGLVSIGCAARLRPVLMTTATTILGMLPTALSLGEG
                     GETMQPLAIVIMGGLTVSTLITLVLIPTIYLIFDKWENKFSDKMPKIFGKITDKIGSI
                     KRKIIKKPKLKAKV"
     gene            13064..14374
                     /locus_tag="ACOIAF_001574"
     CDS             13064..14374
                     /locus_tag="ACOIAF_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HGZ2668588.1"
                     /translation="MNKKMSKIVAIGCGVGIITANLIPVYADNNTSSINKVDNSILSD
                     SNKRTSEKEILTAREAVSAALKNSEKLKMKSEEIKMLKEKLEVQDEFDSFTGSDNSFP
                     YDQIGLLKNQSEQAKGFMEDQIANDITNKFNDLVSRENELDKIKNNLEIKTKEIKDMK
                     LKKDLGLVTSLETESAELELQTLQNTQKAKLQELKNNQDYFKLLTNIDLNNYQLDKEY
                     RFESFRVGGSVDSYMEGKVNEYLKYDQLILERTEESFNDKDENKADLPDRPNLTRPVA
                     PTKPEQGNLTDDEYKVLTDKYEKDYKRYEKEVEAYNLERQTYAAGLTTYANYLQQKFN
                     TENGLVTLEDSKKALKKGLIDSYAQLLALEDTIQITKKQLDLSEKQLKNTKLRYDLGL
                     ITLTDYKKQVVSNEDAKNSYDTLIVNYNSLKNGIEKPWILNTGK"
     gene            complement(14663..14908)
                     /locus_tag="ACOIAF_001575"
     CDS             complement(14663..14908)
                     /locus_tag="ACOIAF_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HGZ2668589.1"
                     /translation="MTKCPKCGKEVSSRPGSICPRCGFKVADESLKIRCPEPSCRALV
                     SRKLDYCPKCGCKLRGYNISEFVNMVRCKIDETFDKK"
     gene            complement(15122..17749)
                     /gene="ppdK"
                     /locus_tag="ACOIAF_001576"
     CDS             complement(15122..17749)
                     /gene="ppdK"
                     /locus_tag="ACOIAF_001576"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HGZ2668590.1"
                     /translation="METKYVYSFGEGSKDMKSLLGGKGANLAEMTKIGLPVPPGFTIT
                     TEACNDYYVNNESIRAEIIKEIEEHLATLEKDLNKTLGCNKNPLLVSVRSGAVFSMPG
                     MMDTILNLGLNDNSVVGLAEATQNERFAYDSYRRFIQMFSDVAMEVPKYKFENVLDRV
                     KEAKGYTVDTELTTDDLKEIVKEFKAIYKKEIKNDFPQDPKEQLMLAIEAVFRSWNNP
                     RAIVYRKLNDIAHNLGTAVNIQSMVFGNMGETSGTGVAFTRNPATGENKLFGEFLMNA
                     QGEDVVAGIRTPQNISTLADVMPAVFDEFVKITHILEGHYKDMQDIEFTIENERLYIL
                     QTRNGKRTAAAAINVAVDLVEAGIIDEKEAIMRIEPNQLDQLLHPKFEDKALKEAKVI
                     AKGLPASPGAASGKVYFNADDVVKANEKGEKVVLVRLETSPEDIEGMVKAEGILTARG
                     GMTSHAAVVARGMGKCCVAGCGEIKVDEFNKEIRALDDVVIKEGEYISIDGSTGNVYL
                     GDVKKTEVSLTGNFEKLMNWVDKHKCMMVRTNADNPRDARAAIEFGAEGIGLCRTEHM
                     FFDEARLPAVREMILSNTVEQREKALEKILPMQREDFVELFKVMDGKPVNIRLLDPPL
                     HEFLPHDDETIEELSKSMGIKVSDIKKRIVDLDEFNPMLGHRGCRLAITYPEICVMQS
                     KAIIQGAIEAIKAGVKVSPEIMVPLVGEVNELKIIRKMIVETVDAIIKEEGIEVPYTV
                     GTMIEIPRACLTADEIAQEADFFSFGTNDLTQMAFGYSRDDAGKFLGQYVDEEILEKD
                     PFQVLDQNGVGKLVKMGAKLGREVKPELKLGICGEHGGEPSSVEFCYSVGLNYVSCSP
                     FRVPIARLAAAQASIKNPR"
     gene            complement(17810..18652)
                     /locus_tag="ACOIAF_001577"
     CDS             complement(17810..18652)
                     /locus_tag="ACOIAF_001577"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HGZ2668591.1"
                     /translation="MLVKNLIIYAVSDSVGETAQQVAKACMSQFYVNETYEIKRFPYM
                     INKGVLLETLENAKAENALIVYTLVDEELCSIVERYCEREGLSCIDLMTDILREISKR
                     TGRKPKREAGIIRKLDESYFKRVEAIEFAVKYDDGKDPRGVLQADIILVGISRTSKTP
                     LSMYLANKNIKVANVPLVPEIPIPKEVFEIDTKKIIGLTNSPEKLNEIRTQRLKALGL
                     SSKANYANLERILQELDYSEEIMKRIGCPVINVSNKAIEETAGIILDIMKENGLKIYK
                     EIEI"
     gene            complement(18659..19297)
                     /locus_tag="ACOIAF_001578"
     CDS             complement(18659..19297)
                     /locus_tag="ACOIAF_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGZ2668592.1"
                     /translation="MIIIQLNERQLKIIDIVKENEPITSESIASSLNVTRATLRSDLA
                     ILTMTGILDARPKVGYFYSGVSEINLIGKSVKEKTVEDIMSMPVLAKKDESIYDVIVT
                     MFLSDVGSIVIIDENEELCGVVSRKDLLKATIGGSDINKMPIGMIMTRTPNVVTLTKG
                     ASVLLASRKIIEHEVDSIPIVEYKGEDKNHMRVVGRISKTNITKLFLEIVDN"
     gene            complement(19733..21025)
                     /locus_tag="ACOIAF_001579"
     CDS             complement(19733..21025)
                     /locus_tag="ACOIAF_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HGZ2668593.1"
                     /translation="MLDLNRKLAKLEEEGKKIKVALIGAGHMGNGMVSQMANMKGIEA
                     SIVVDINLELAHKAFTDAGIDEEIIDNVTNEKDAEIKLQEGKVLTCNDFLVACKTKSI
                     DVVIDATGGIAIGAEIALNSILNKKHIVMLNAETDCVVGPILKKLADDAGVIFTGSAG
                     DEPGAVMELFDFADAMGFEVRVIGKGKNNKLDLDCNPDTVREEAERKGASPHMIASFK
                     EGTKTMVEMALMCNATGFVPDVRGGHGIEATVNEVPKKYALKSEGGVLDNYGVVDFVN
                     GIAPGVFVVVAHKLKAVNDELKYLSMGDGPNYILYRPYHLCSLETPLSAAMAVLDHKA
                     TIVPKAGLVAEVMTIAKKDLKKGEFMDGYGAYTCYGTIEKYDVAKAMNAVPIGLISKK
                     TKVVKDIKKGEVITYDMVEIEKDTTLYHLRQLQEKIFG"
     gene            complement(21062..21418)
                     /locus_tag="ACOIAF_001580"
     CDS             complement(21062..21418)
                     /locus_tag="ACOIAF_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HGZ2668594.1"
                     /translation="MKYEVKISGIGSLVKELMDESNCLIIYDETINDDDLKDISVIHS
                     IATLKSDVEIGDTLTIGNRDYCIVSVGDIAQKTLREIGHCTIKFDGKCEVNLPGEIHV
                     EIGNPDITIGDLITIS"
     gene            complement(21453..21812)
                     /locus_tag="ACOIAF_001581"
     CDS             complement(21453..21812)
                     /locus_tag="ACOIAF_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HGZ2668595.1"
                     /translation="MKLLVILFVAYILNTIFIMKQNKLYFKTLNRAKKKGDIVSTGKK
                     KSYFSKGSIAIISSDSDGFIKFGEILKGRTVMAKFKEIEEIEGLDIYSAEQKFKDEES
                     IVQAIKFIKEKINVSFN"
     gene            complement(21881..23854)
                     /locus_tag="ACOIAF_001582"
     CDS             complement(21881..23854)
                     /locus_tag="ACOIAF_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HGZ2668596.1"
                     /translation="MSVTEKIPSIDRKIFKILIMCSKKEFVSINSIANELNVTTRSVR
                     TYIKQLNKDLGNDIAVIKYIKGQGYKLEIKDEQILNKIIDMNRKNVFSLNSKEDRVEF
                     ILNYLIELDGFITLDSLADEMCVGRTTLVNDFQYVEKVLASYNLNLIKKQNTGMKLNG
                     NELDIRLFILNQLYKNSRKDFSNSKYFKGIKKEEIINLEEKLLTLFKKNNFYVTDEML
                     REVINYIVVLVYRVKEEKKVKDYDVKFDLLKSYDEYFIAREIKHIISGMFECTLNDEE
                     IIYLTIPLVSGNAPASECALNSSRISKNIDELMENIFNQIYVDMGIFINEDELRVGLG
                     YHLSFTLNRLLFNIKLKNVLLEEIKQNYILPFKLAQIAGKVIEKKYNLEVSEDEIGYI
                     AIHFSGYLERNSSRFYSIKKIAIICSTGLGTAKLLKIRVEKLIGNNPKIDTLSSFNLR
                     NINLDEYDIVFTTIDLDTSNINTIVLKINTIFDENKLREQLKTVLCLREGNIDTTNST
                     NLLINNLLDEDKFMILNEKTILASLEKMMDHLMDLGCIDDKFRKNIVNREEKSPTVFD
                     KGLLFPHSVNEKSDKFLMAVGILEEPIEYANRSIKIILMTMFPCENKIDSDLLVKIYE
                     EVLKIGQDVKLTNKISKCRTFLEFKKVLLKNLI"
     gene            complement(24076..25089)
                     /locus_tag="ACOIAF_001583"
     CDS             complement(24076..25089)
                     /locus_tag="ACOIAF_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HGZ2668597.1"
                     /translation="MEENKILKIEKGTSGWGGPLYIKKEGNRNKILSMTAGGIHEVTL
                     KIKELLGCEIVDGFKTGVSDEEVAVVIIDCGGTARCGVYPKKKIPTINVNPVGKTGPL
                     AKFITEEYYVSDVNPNCISVVDGEDMPQKSQENKSENKSSIRKPDNYDEVKSKAQGEY
                     AKKNIILSIGQGAGQVVSKFYDAGRDTIQMVMNNVIPFMAFVSMLMGIILASGLGDWI
                     ARVISPLAGNIGGLLIISVICTLPFLSPILGPGAVIAQVVGTLVGTQIGLGAIPAYLA
                     LPALFAINGQAGCDFVPVGLSLGEAEPETVEYGVPALFYSRLITGPIAVIIAYGVAVF
                     ALR"
     gene            complement(25102..25647)
                     /locus_tag="ACOIAF_001584"
     CDS             complement(25102..25647)
                     /locus_tag="ACOIAF_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HGZ2668598.1"
                     /translation="MENVITGLSKGAEWFIGLFQKGGEQFIGLVSGTLPTLIVLMVAI
                     NSLIKIIGEERVNNWASKLGKNSFTRYILLPLVSVFFLGNPMCYTFGRFLKEEHKAGF
                     YDAAVSFVHPITGLFPHANAGELFVWLGISAGLTTLGKETTTLALWYFIVGLIVIFIR
                     GIVTEKMYAFLTRKNRATNKA"
     gene            complement(26023..26892)
                     /locus_tag="ACOIAF_001585"
     CDS             complement(26023..26892)
                     /locus_tag="ACOIAF_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HGZ2668599.1"
                     /translation="MDYLKYFEKAVMYIENNLHENITVNDVAKETGYSYYHLTRLFKS
                     MFGESVGSYIKKRRLVSSTKELLYSDKKVIDIAIGSGFESSEAFSRAFKSIYKVSPIE
                     YRKNRIDVFVGKKKKLELDFMRHLVGNITIKPVIKEIEKIKVIGIKDKVILENNSLPD
                     LWEKFRKVHHIVPNTLPSKRVFGICEAISEIHLVSESMEFNEIIGLEVNSYDVIPNSF
                     VSKTIKEGKYAIFTHTGSLDNLDKTYEYIWGTWFLSSKEELDIRDDFEVYDERFLGPS
                     NVNSQIDIYIPIK"
     gene            complement(27044..28159)
                     /locus_tag="ACOIAF_001586"
     CDS             complement(27044..28159)
                     /locus_tag="ACOIAF_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HGZ2668600.1"
                     /translation="METLEVRNIELSGTNYEIGYRLGELVANMPEIIEGQINKSNLVS
                     KKEEKEMIELFDKYCPGLNEELQGFADAIQVNCNQILYYTMTYLKPGCSQVALAPELT
                     ENGHVLFARNFDFSHNMEDFVLCKTKVNGKYAHIGTTIMQFGRGEGMNECGLGVSQSS
                     CGIPVGNSDGLRKPAIVGLQFWAVIRYLLENCKDVDEALEYLKDMPIAYNINLLLADK
                     SGNIALVETLDGKKEVNIINSLESKREYFLHSTNHTHIDKLHKLDPQSMKNSIHRYKL
                     IKEYINKSKKIGEKELMNLLSSKYPNGLSCNYYNDFFGTLKSIVMDLNIGKFNILWGG
                     LENKWESYYLKNDIKSITQRININIEKAPSDFFDFIE"
     gene            complement(28395..29531)
                     /locus_tag="ACOIAF_001587"
     CDS             complement(28395..29531)
                     /locus_tag="ACOIAF_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HGZ2668601.1"
                     /translation="MKERIIQVADSKKEKILGLCQSLYDEPEIALQEYKSAKKISEFL
                     REEGFDVEENLAGMATAFKATKKNGDGPKIAFIAEYDALPGNGHACGHHLIASMGVGA
                     GIALSSILDTYKGEVSIIGTPAEETGDGKPYLIEHGVFDGYDAAMMIHPNSKTCVTPE
                     IIAIGGLDFIFTGKASHAGAKPYNGINALDAVVLLYNNINALRQQLVDGTRIHGIILE
                     AGTAANVIPDMGKVRLEIRAKEQNYFDEVVEKVKNCARGAAIATGCELEFYHFEPTCQ
                     GLNENKVLVDIFTKIMEEFGIYEDEQVMLGSTDMGNLSQIMPCIHPLMKFSENGEELH
                     TKEFLEASINSYAKDRVIDGIKILALTGFNLFENPELLKKMKEE"
     gene            complement(29587..31050)
                     /locus_tag="ACOIAF_001588"
     CDS             complement(29587..31050)
                     /locus_tag="ACOIAF_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HGZ2668602.1"
                     /translation="MTDLVNTINGIVWSPVLVAMCLCIGLYFSLKLKFFQIRDVKEMF
                     TLLLEGDSSHVGISSFQGFATSMAGRIGTGNIAGVAVAIAMGGPGALVWMCIMATLGS
                     ATAFVESTLAQIYKDEHDGQYRGGPPYYFEKGLGWKKCSVLFSIVAIISYTFFMPGTQ
                     SNTLYLAMNQAFGFSKMTIAVASSIALAIIIFGGIKRIGLFAEKVVPIMGGAYLIMTL
                     VIIITNISLVPEAIVTMFKSAFGFGSVFGGMLGSAISWGVKRGIYSNEAGEGSGTYGA
                     AAAEVSHPAKQGLVQAFSVYVDTLLICMATGIMIVITGMYNVTGVCTALPGVEPGPQY
                     VQSAINTLHPQLGDIFIAIVLLLFTFTTLLAFSYMMETNVSYLVTRIKAEKHEKYIIN
                     VCRFMLIACAAMCCFIEPLTSWALGDICIGVLTYINLIAIFTLKKPAIKAYYDYIRQK
                     KMGIKRSDRTFNPIELDIRNAEFWENRYLKEDKHELN"
BASE COUNT        10616 a         5292 c         3827 g        11541 t
ORIGIN      
        1 gtatattatt tttatattaa tttaatttta tcttgattca gttttataaa aaattgttat
       61 aattgtcaag ttctactcta ttatttataa tttcttatat cttattaaca acatttaagt
      121 tattacattt atttatatat attttttcat tatctaagtc ttcatcaaac atttttatta
      181 tattttttat ctcatacttg tactcatctt cattcattct ataaagcatt aaatcttcta
      241 gtacatcttt catatcgaag tacacatctt ctaataaata aaaatagcct tcctctatat
      301 ctttcaattt atctatagta tccatttttt tacttatatc tttcataatt tctaaatttt
      361 gtatatttac atctatgatt gttaataagt acattctcac agtttttatt atttcaacag
      421 aaaaatctac aatattataa tttccttctt ttttatataa tgactcctta aatttgttaa
      481 aacaattatc taaattagaa tttatatcgt taaatggtgg tcttattatg tcacgtatta
      541 ttaaaagatt ttgtatgtct acattatttg tgattgcctc atccaaaata aataaattat
      601 atgcatcatt taatgggtca tgggcatctc cactaaactc taagttaaca aattcaagta
      661 aatttttcaa tgatatatag ttcatacatc tcataccaca atttatatat ttttctttta
      721 tatctacaaa taaatctcta atgttattta caaaccttgg atggttattt tttttagcac
      781 ttctcatatc tgtgttattg aaacatgtca aatccaaatt accaaaagta tatattcctt
      841 taatatcaga aaatatgcca agccattttt taaactttct catgacctct tcataacttg
      901 gtgcactctt caattcttca gaagatatat gtgttaattc ttgtatatga ggatacaact
      961 cctcattact tactggtctt attaaagatt taaatttatc aatatttttt gttttttcat
     1021 catacataac tgctccgatt gcaattatat cagaattaaa tactgctctt tttgatttag
     1081 aatttggcat attcatttca aaatctatat atactctttt caatataaac accctttcca
     1141 attgtttata gtgattcttt ttaactattt ttattcaaaa ttttctaaga aagaatgttt
     1201 ctccccatct ttttgccaac caagtactga atcaagattt acattttctg ccgccttaaa
     1261 tatagatttt tctacatttt taaaatttga ttctaagctc tttattaagt cttttatctc
     1321 atatctttta ttacctgttt ttttagcagc aaccatacat gcacaattta atggccatat
     1381 accactattt tgaataaatc ttataatatg ttcttctctt atataataaa gtggtcttat
     1441 aatttttata ccttcaaaat tagtagaatt aagtttagga agcattgttt taaaatttcc
     1501 agcacacaat aaattaagca ttgttgtctc tattacatcg tcataatgat gacctaatgc
     1561 aagtttgtta caccctaatt cttctgcctt tgaatataat gctcctctcc tcattcttgc
     1621 acacatatag catggataat cttttgctat ttcatctgct atctcaaata tccttgaatc
     1681 aaataaatgt attggtatat ttagatactc acaattatct ataagtaatt gtcttatatt
     1741 tgcatgatat ccagggtcca tagcaataaa ttcaacatca aaattaactt gtccatgttt
     1801 ttttaactct tgaaacattt tagccataag aatactgtct tttccaccag aaatcgctac
     1861 agctatctta tcaccttctt caaccaattt ataatctctt attgccttca taaacttaga
     1921 ccataaattt ttcctatatt tttttattat acttttttct atatctctta ctggaagtct
     1981 ttcctcaaaa ggaactataa tttcgcagcc ttttcctgat agttcactca tctaatcacc
     2041 tcgtatattt atgttaataa tattttacaa aaaagtctac cttcatccac aaaatactat
     2101 aatttttact ttaattttac taaataattt atagtttaca ataacacata aacttataat
     2161 aacataaata acctctttct tttattatct tttttatttt tctcatttaa tattttatta
     2221 ttagtaacga tttttaaatg attgaatatt atacactaga tagagttacc tatctattta
     2281 caacaaggag gttttcatct tggaaaataa gactagagat atcaaaaaaa attgtggtcc
     2341 atctttggct agaccataca ttaataatca aattttcaca gaaatgtact gcctttctga
     2401 tgcattaaaa tttggaacta tatttccaga actaaactta ctagagtctg aaatgtacaa
     2461 caaagaatta tatgctaagc caaaaaaata tagaggaggt agaagataat gaaagactca
     2521 tctagagctg aacttatgag aaaagttcag gaaacatctt ttgcatgtgt tgatatgaac
     2581 ttatatttag acaatcaccc tgatgataaa aatgctatca acacttataa ttcattatgt
     2641 aatcaatttg cgcaagctag atatgcttac gaaaataaat acggtccttt aactaacttt
     2701 gggtatgctc caagtagatg cccttggcaa tgggctgacc aaccttggcc ttgggacaga
     2761 gaatttaatt attagacatc aagtattgga ggattgaaaa ttcatgtgga tatatcagaa
     2821 aactatacaa cacccagtta atataaaaac ttgtgaccct agaatggcta aatttcttat
     2881 aactcaattt ggtgggccaa atggggaact tgctgcatct ttaagatatt taagccaaag
     2941 atatacaatg cctactggta atatgcgtgc acttttaaca gatattggta cagaagaact
     3001 agctcacgtt gagcttatat gtactatggt ttatcagtta acttctgatg caagcccaga
     3061 agagttaaaa gctgcaggtc ttggttcaaa ctatgctcaa aatggatatg gaatttatcc
     3121 aacagattca aatggtgttc catttgatgt aagacctata gcagttatgt caaatcccgt
     3181 aaccgattta catgaggata tggcagctga acaaaaagca cttgcaactt attatcaact
     3241 tataaaccta acagatgacg ttgatgttat agatgtatta aaattcttgg gtcaaagaga
     3301 aataattcac tatcaaagat ttggtgaagc tttaatggat gcttacgagt tagaagaatc
     3361 tcaaaaaatg ttctaaaatt tttatttgta taactattat tatttgataa tttataattt
     3421 actgttgtgt taaaatttat aaaaaatata aatataattt attttctata aacagagaaa
     3481 ataaataaaa agcaggtatc aatcaaatag taacatgtat tatactatac attactatca
     3541 atattgtacc tgctttcata ttataagcaa taaagaaaaa tctatttatt aggttttata
     3601 ataaaaacaa ttataaaact cttcttgcat ttacatatct acttgaatag tagctactat
     3661 ttatattaga tattgttact ttttttgatg aacctgaaga tgcatgtatc atatctccat
     3721 ttcctatgta tattccaaca tgacttacag aaccattatt tgaaccttgt gtatcaaaga
     3781 atactaaatc tccacttcta aggtctcctc tatttacata tgttccatac ttactttggt
     3841 ctctagatac tcttggtata ctaactccta cagatttttt cataacgtac tgtgtgaatc
     3901 cagaacagtc aaaactattt ggaccttctg ctccccatac atatggtttt ccaagtagtg
     3961 tctttgcaaa acttattaca ctatcagctc cttgacttgg agaattagtc gaataagatg
     4021 gaattgaagt atcaacagaa cctttttcag caaggtattg acttgacaca tatccagttt
     4081 gattgccata acttactttt gtccatcctc cactttcaga aatacttcca acctcagtac
     4141 cataacttaa tgtagctatt ttagaatgac ttgtacctgg tcctgttcta acgttcaatg
     4201 actttgctgt tacaaccttt gtacccttta caaggtcatt tgaactgttt tcagcatttt
     4261 catttgtaga gtcatttaca ctagttccta aatatttact tgaaacataa cctactctac
     4321 cattagaact tatttttgac catccatttg actcagatat gtatccgact tcagtcccat
     4381 atcctagtgt tgatatttta gaactacttg tacttggtcc tgttctaaaa tttaatcctt
     4441 ttgctgttac aacttttgtc gacttaactg aagtatcact actttcattg ctacttccaa
     4501 ggctatttat tgctacataa tttccatgca cataacctgt gtttccatta tatttaactt
     4561 ttacccaaga accttctttt cctacgtact caactttatc acctttgtaa agtttcccca
     4621 ttgattcatg gtttgtacct ggtccttttc taaaatttac cgcattccct gttattgttc
     4681 tgctttctat actcacactc tctacttcag actcattagc atatgctaaa ttccccatag
     4741 taggcattat tatcgcagtt gccattatac tggctgctat tctcttttga ttaaaattca
     4801 taactttcca ctcatctcct cttttctact atctttattt cacttcaaaa tataaaagaa
     4861 ataatcgaca ctatcaaaat atcaattatt tcttttatat taatattatt acaaatttgt
     4921 aactatttgt ctatagtaat atttgcttac cataaattac catgtataaa ttttaatctt
     4981 taatagcctg taatttagta atatagccat cttttacctt ttttaaaaat ggatatattt
     5041 tttaactttt ttgtaacttt ttaaaatcca gttgagccaa acccaccttt tcctctttcg
     5101 gagtcactta attcttttac ttcttctatg ttaatatcat agataggttt tactatcatt
     5161 tgagcaattt tcatatgctt ttcaacctta aaatcatttt taccatgatt tattaatata
     5221 attttaagct ctcctctgta tccttcgtca atagttcctg gtgtatttaa tacagtaaca
     5281 gaatgcttta aggcaagccc acttctaggt cttacctgcg cttctgtcat agtaggaagc
     5341 tctatacata tcccagtttt gataagttta gtctctccag gaggtattac aacctcttct
     5401 atagaataca aatccatacc cgcatcacct ttgtgagcaa aattaggtat tatagcatca
     5461 tcatttaatt tttttacttt taaattgtac atcttatatc tcctttcaac tttctataaa
     5521 tattataact taaatttcga cctcttgaag ttttttattt ttttgtggta atataattat
     5581 tagtgtttta aaggaggatt ttttatgaaa cttgtacttg ctgagaaacc atctgtggca
     5641 aaaactatag catcattttt aggtgcaaag accagacaag atggttactt tgaaggaaat
     5701 gattatataa ttacttatgc agttggacac ttagtatcat tatatgatat gaaggattat
     5761 gataaagata aatactctgg ctcttggaaa atgaacaatt tcccatttgt tcctgaggat
     5821 aagtttaaat ttaagattga tagttctaaa actaaacaat tcaacactgt aaaaaagctt
     5881 ttgaatagaa aagatgtaga atatgtaata aatgccactg ataatgaccg agaaggagaa
     5941 cttatagcat ttttaatatt tttactggca aaaaataaaa aaccagtaaa aagaatcttg
     6001 gtaaatgaat ggacaccaga agatattaca agaggtataa aaaacctaaa agatgaagat
     6061 gaaatgagaa atcttcaagc tgctggatat actaggctta ttactgactg gttgattgga
     6121 ataaatttca cttctgttgc tacattgaaa tatggaaatg ggaaactatt aaacataggt
     6181 agggtaattc ttccaacagt taaattagtc tatgatagag atatggaaat actaaacttt
     6241 gttcctaaaa cttattatga aatagaaggg cattttaaag ctgaagctgg tgaatataaa
     6301 ggtaaatatg ttaagggtaa agaaagtaaa tttgatacac ttgaagatgc aaataaaatt
     6361 atagcttcaa ttacatctga aactggtaaa atactagata aaaaagttac tatgtcaaag
     6421 gaatatgcac caaaactttt tagtttgaca tcacttcaag gatatataac ttctaagtat
     6481 tccaatttta catctgataa ggttttaaat gtctgtcaat ctctctatga gggtaatggt
     6541 aagggtggat acataactta ccctagaact gattccattt tcttagaaga aagtttggct
     6601 tcaaaagctt ctcaaacttt agataaatta aaggttggtc ttgaatatga aaataaaatt
     6661 aaattttcta aaactaaaag agtttttgat tcttctaaag tagactccca tagtgctata
     6721 attccaactt atataattcc aaaaagttta tctaaggatg aacaattagt ttatgatgcc
     6781 ataaaagata gatttgtagc aaattttatg cctcctgctg aatatgagaa tacagaaatt
     6841 aagactgaag tagataattg tactttctta acaaaaggta aggtgcttaa atcaaaagga
     6901 taccttgagg tatataataa ggaagaaaaa aacgatttac ttccacttgt aaataaagat
     6961 gatgttgtag atgtgttaga aataaaacca ttaactaagc aaactactcc acctaaaccg
     7021 tatactgagg acaccctttt aaaggctatg aaaaattgtg gtaaaaatgt gcctgaagaa
     7081 gatacaacag tgctatctgg atattcgatt ggaacatcag ccacacgtgc tgatgtgtta
     7141 aagaagataa gtcaagtagg ctatgtaaag aaaaaaggga aatcttattc tataacagaa
     7201 cttgggaaaa atttagttga aatttttcct gttaaagact tatttgatgt agattatact
     7261 ggaaaacttg aaaaaagttt aagcgatatt caaaaaggaa agtatacaag aaaagaatat
     7321 cttactaata tcattagttt tatatatcaa aatgtcaata ttataaaaca agatgcttca
     7381 aaaaatataa acacagaagc atatactttc gattcaaaaa ctaaaaaatt tactaaaaca
     7441 aaagaaaaaa aacagaaaaa ggatgtaact aatactgaaa aagaagttgc tatatcaaag
     7501 gattctaata acaaaaaaga agttaaaaat ggtgatgaaa atcaatctct tggcaaatgc
     7561 cccatttgtc aaggtgatgt actagaattt gacaaaggct ttgcttgtaa aaatcataaa
     7621 gaatgtaaat ttgttatttg gaaaaatgat aagtatttaa gtctttataa aaagaaagtt
     7681 aacaaaacaa tggtcaaaaa tattttaaaa aaaggtgaaa ctaaagtaaa atctttaact
     7741 gctaaaaacg gtaataaatt tgatgctaca ttaaaatatg ataaaaatcc agatactggc
     7801 tatttcaatt ggaaaataga gtttgacaac taaatattca taagaaaaga aatagcagta
     7861 gtataataat ctatattata ttactgctat ttcttttctt atgaagtttt aactttttta
     7921 atagtgactt ttacaatcac actctaacct aatttcttta ctatttctga taactttaat
     7981 gtatgttcat ctaataaagt tagttcctct tcagaaagat tagataattt ttttactaga
     8041 gattctttga gaatagcttt tactgcatca aataatttaa atgctttatc tgttaattca
     8101 accctaagaa tccttctatc ttttgggtca ggaaatttat tgactaaacc atgttccact
     8161 aatctgtcaa taatcggagt catatttgat tttaaaattc ctaaattaga agctatttga
     8221 gaaattgatg atgaatttga tgtcataaga taaagaatta ccttagcatg tgatggagcc
     8281 attggacatt cttcaaaata agctttcatt atatcagagt tattttgaaa cttttttaac
     8341 aaatcgcttt ctttaaatat attattttca agcacaaata aaaaatctaa aaaagagtcc
     8401 gctattttaa gtatattgtt gttatccata tttgtaacat cccttcaata gataatataa
     8461 taaaacattg ttgtttaata tagtataaat tataaactat taataaattt aaaacaagaa
     8521 aaagttattg actcataata attataaatt tataatcaaa ttagagaatt ataaattgcc
     8581 aataaaggga agttttttaa ttagtctaaa actaaactta aattataaga cactttaaat
     8641 tctacaacaa gctagtgtgc aatatataat aggaggagat atgaaaagat atttaatagt
     8701 tttcttaatg tgtagttcac tgtttttagt tggatgtgga aaaacagaag aatctgaacc
     8761 tgagaaacca atagcagttt ctgttcaaaa agcaataggt ggagaaattg aaaacacaaa
     8821 ttctttctca ggaactacaa aggttaaaga tgaaacagct gttactgcac agactgttgg
     8881 cacagttcaa gaagtgtatg taaaacttgg gcaaaatgta agaaaaggag atgaattatt
     8941 gagtataagc agtccagagc tagaaaatag tgtaaagcaa agtaaagcct cactagattt
     9001 agcaaaagca agttattcaa gtgccactgg tggttcatta gaggcacaag taaatcaggc
     9061 aaaaacagct cttgacaatg caaagattca atatgaggag tctcaaagaa attatgacaa
     9121 taataagatt ctttatgaac aggaagtcat tagtttagac caatttaaaa aaatagaatt
     9181 ttctttagag caaacaaaac aacagttaga ttctgctcaa agagcatacg atacagcaac
     9241 ttcaaaaagt atacctcaag ctaaagcact tgctaaaaaa cagcttgacc aagctcaagt
     9301 atcatataat ttagcaatga gtaatttaga caaattaaca ttaacatctc cagtagatgg
     9361 tacaataaca gctaaaaact ttgattcaaa ggaaatgata acacaatcac aaccagcttt
     9421 tattatttct aatcctaata tactagaagt tgatttaaat gtagcagaat cagatatagg
     9481 taaatttaag aaagatggaa atgtagatgt tataattgaa gaccaaagga ttttaggaaa
     9541 aatagattat gtaccttcag tagttgaccc tcagacatct ttatatcctg ttaaagtact
     9601 tgtaaataat acaaacaaca aatttaaggc aggtatgtct gctcaagtaa acttaagtgt
     9661 tgaaaaagaa aatggtgctg ttacagtacc taaaaaagca atatttgaag aaaatggtaa
     9721 aaaatatgta tacatagcaa caaaagacaa tatagcaaag aaacacttgg ttcaaactgg
     9781 aattgtaaca gaggataaaa tagaaattaa aagtggtgta agtgataaag atacagtgat
     9841 tatcggaggg ataagtctta tttctgacgg aactaagata tttcctgtag aaaaggagaa
     9901 gtagactatg aatttaacac agacttccgt taaacgtcct ctcacaatca taatggtctt
     9961 tttagtatta atagtttttg gtggtattgg atataaaaaa atgtcaataa atctaatgcc
    10021 tgatatagaa ataccagttg taatggtcat gactacttgg acaggtgctg gtcctcaaga
    10081 cgttgatgaa caagttagtc aaaaagttga tgaaagttta tctgccgttt caaatgtaaa
    10141 atctactata tcaagttcac aagaaagtgt ttctatggta gttgctcagt ttgaatttgg
    10201 aactaatttg gatgaaataa tgaatgatgt taggtctaaa gttgatgcat tacagaccag
    10261 cctcccagat gatgcagcta aaccaacagt tttaaagctg gatatgaatg cacaggccat
    10321 aggtcaactt gtcatatctg gaggaaatga aaatagttca caagccttaa gaaaatacgc
    10381 tgaagatgta attcagccta aaatagaatc catagatggt gttacttcag cagaccttaa
    10441 aggtggaaaa aaagcacagg ttaatgtaat agctgaccca gctgttcttt caaattatgg
    10501 agttagttta tccactataa aaggtgtctt atcctctagt aataaaacat tcccttatgg
    10561 ttcaattact caaggtgagg ataaaatagt tttaagagcg attgataaat tagaatccct
    10621 agatgatata aaacagattc aaattcctgt aaaaggagga aatacagtta gattagataa
    10681 tctttgtagt gtagattatg gatatgcaga caaagatagt atttatagat acaatggaaa
    10741 agacagtttg gtaatagata tttctaaaca acaagatgcc aacactgtaa aagttatgca
    10801 gagtgcatac aaatacgtag atgaattgaa taaggaaaat cctcaattta aaattaaaat
    10861 tgctaatgat acaagttcat acataagtga atcaataaac agtgtaatga gcaacttgct
    10921 tataagtgct gttatcgcat ttattgtaat atttgctttc ctaaaaagtg taagagcttc
    10981 actagttgtt gcagttgcaa taccaatttc tataataggt gctatagcaa ttctctactt
    11041 tactggtgaa agtttaaatc ttattactgc aagttcattg gtaatttcag ttggtatggt
    11101 tgttgataat gctacagttg taattgaaaa tatatttaaa tataggaaaa gtggcagatt
    11161 gagtcttgat gattgtgcta ttgagggaac aagaactgta acaaatgcaa tacttgcatc
    11221 aacattgacc acaatagcaa tattcttacc tatcatgttt acagaaggta tagctaaaat
    11281 aacttttggg gcattaggta aaacactaat agcttcacta gcattctcct ttataggtgc
    11341 aattacactc gttcctagtg tatttgcaaa attaagcaga ggaaaaaata gtcaaaagat
    11401 gcaagaaaaa gatagtccta tatttgataa ggtaagtgaa gcatataaaa aattattaag
    11461 tgtatgtttg agacatcaac gcttagttgt aatatcaagt ttacttattt ttgtagcttc
    11521 cttatttgga atgggattta taggtatgga ctttatgcct gcagcagaca agggtgaatt
    11581 atctatttct atagaacttc caaaaggtct tagcctagaa tccaatgatt actatgtatc
    11641 tatgactgag aaaaaagcct cagatattcc agaaattaaa actttaataa caactttgac
    11701 atcagattca aataatagta aagcaagtat agcaattgaa ttagaatctc aaaaaaatag
    11761 aaaaagaact actgatgaaa tagaaaaaga gataattgat agagtggcta ctgtaccaga
    11821 ttgtaaaata aatgtaagtc aaaacagtag tgttatgggt ggagctggag gagcagactt
    11881 cacattagaa atgaaaggtc ctgatttaga tgtattaaaa atactgggaa aagaagctga
    11941 aaaagaattt agctctataa aaggatttag aaatgttgaa acttctctta cagatacaac
    12001 acaagaagct caatttacta tagataaaca taaggctcaa gaatatggtg taaatactgc
    12061 tgaaattgca ggcatgttac gtacagcaat aaatggtgac tccgtaacca ctgctactat
    12121 tgatgattac aaagtggatg taaacctaaa atttaaagca gatactattg atagcttaga
    12181 agatataaag caactaaaag taaactctgc aactggaaaa gaagttcctc ttggtgcttt
    12241 ttcagatata aaaatggcag atggtctaca atctatatct aggactgatg gagatttttc
    12301 tgtaaatata actgctacac ttgacaattt agatgctggt actgctacaa aactagcact
    12361 agaaaaaacg aataaaatgg taattcctaa agattactct ataggtgttg gtggtgaaac
    12421 agaaatgatg aatgaatcta tggcaagtct tggattttca atggtcatag ctatagcatt
    12481 agtttatatg gtaatggtag ctcagtttga atcatttagt aaaccattta taatcatgtt
    12541 ctgtgttcca tttgcattcg ttggagttgt gctttcgctt ctagttagtg gtcaaagttt
    12601 aagtactgtc ggtatgctag gtataatctt acttattggt attgttgtta ataatggtat
    12661 agttcttatt gactacatag agcaacttag aaaaacaaat cgtaacaatg atttaattgg
    12721 tttggtttca attggttgtg cagcaagatt aagaccagtc ttaatgacta cagcaacaac
    12781 aattttaggt atgttgccaa cagcactatc tcttggtgaa ggtggagaaa ctatgcaacc
    12841 actagccata gttataatgg gtggtctaac tgtatctaca cttataactt tggtgcttat
    12901 acctactata tacttaatat ttgataaatg ggaaaataag ttttcagaca aaatgcctaa
    12961 aatatttggt aagataacag ataaaatagg aagtataaaa cgtaaaataa taaagaaacc
    13021 aaaattgaaa gctaaagttt aaatagaatg gagggtgatt gtattgaata aaaaaatgag
    13081 caaaatagtt gcaattggct gtggtgttgg tataataact gccaatttaa tcccagttta
    13141 cgcagataat aatacttctt ctataaacaa agttgataat tctattttaa gtgactcaaa
    13201 taaaagaaca tctgaaaagg aaattttgac tgctagagaa gctgttagtg ctgcacttaa
    13261 aaatagtgaa aaattaaaaa tgaaatcaga agaaataaaa atgttaaaag aaaagttaga
    13321 agtacaagat gagtttgata gttttactgg aagtgacaac tcgtttccat acgaccaaat
    13381 tggacttctt aaaaatcaaa gtgaacaggc taaaggcttt atggaggacc aaatagcaaa
    13441 tgatattact aataaattta acgatttagt ttcaagagaa aatgagctag ataaaataaa
    13501 aaacaatctt gaaataaaaa ctaaagaaat taaagatatg aaattaaaaa aagatttagg
    13561 gttagtaaca tctttagaaa ctgaatcagc tgaacttgaa cttcaaacac tacaaaatac
    13621 tcaaaaagct aaacttcaag aactaaaaaa caatcaagac tattttaagt tattaactaa
    13681 tatagattta aataattatc aattggataa agaatataga tttgaatctt ttagagtagg
    13741 tggctcagtt gattcatata tggaaggtaa ggttaatgaa tatttaaaat acgaccaatt
    13801 gattttagaa cgtacagaag aaagctttaa tgataaagat gagaacaaag cagatttacc
    13861 agatagacct aatcttacaa gaccagttgc tcctacaaaa ccagagcaag gtaacctaac
    13921 tgatgatgaa tataaggttc ttacggataa gtatgaaaaa gactacaaga gatatgaaaa
    13981 agaagttgaa gcatacaatc ttgaaagaca gacttatgct gctggcctta ctacttatgc
    14041 aaattatctt caacaaaaat ttaataccga aaatggtcta gtaacattag aagacagtaa
    14101 aaaagctctt aaaaaagggt taattgatag ctatgctcaa ttacttgctt tagaagatac
    14161 aattcagatt actaaaaaac aacttgattt atcagaaaaa caattaaaaa atactaaatt
    14221 aagatatgat ttaggattaa ttactctaac tgattataaa aaacaagtgg taagtaatga
    14281 agatgctaaa aatagctatg acactcttat tgtgaattat aactcactaa aaaatggtat
    14341 agaaaaacct tggatactta atactggaaa ataattctgg taaatagaaa gattacttta
    14401 acatacgttt gattaaaaaa tagttatgca cactcgttgc tttagtcatg gaagattcag
    14461 aaggcaaata atagaaatag attagtaaaa atatatttac ttataaaact gtatactttt
    14521 atagaggcta tctcaaaata gagataggcc tcttttttat tgtttaaatt ataaaaatat
    14581 atttgattcc atacaaaaag agtgccttat gaactaaaaa gttcattttg agacaccctc
    14641 aaaagcttaa atctaaattt aattattttt tatcaaatgt ctcatctatt ttacatctca
    14701 ccatgttaac aaattcagaa atattatatc ctctaagttt gcatccacat ttaggacaat
    14761 aatccagttt cctagatacc aaagccctac aactaggttc tggacatctt atttttagac
    14821 tctcatctgc tactttaaat ccacatcgtg ggcatatact acctggacgt gatgatactt
    14881 cctttccaca tttaggacac tttgtcaact ttatcccctc cagtaaactc aatttaattg
    14941 atagttgaaa tttctctcta caataatata tgcaatataa ttaaatacgt ttcacttaca
    15001 actatataag ttaaattttt ataataaaaa tcccctatat tcaatttata aatacttaat
    15061 ttgatacatt gaacaatagg ggattttatt tttatttatt tacatatttt gcagtctatt
    15121 attatctagg attttttata gatgcttgag ctgctgcaag tctagcaatt ggcactctaa
    15181 atggtgaaca agatacatag ttaagtccta cactataaca gaactctact gaagatggtt
    15241 ctcctccatg ctctccacat attcctagtt ttaattctgg tttaacttct ctacctaatt
    15301 tagctcccat cttaactaac ttaccaacac cattttggtc tagtacttgg aatgggtctt
    15361 tttctaatat ttcttcatct acatattgac ctaagaattt accagcatca tctcttgaat
    15421 atccaaatgc catttgagtt aagtcattag taccaaaact gaagaagtct gcttcttgtg
    15481 ctatttcatc agcagttaaa caagctctag gtatttctat catagtacct acagtataag
    15541 gaacttctat accttcttct tttattatag cgtcaacagt ttctactatc atttttctta
    15601 ttatttttaa ctcatttact tctccaacta atggaaccat tatctctgga gaaactttaa
    15661 cacctgcttt gattgcttct atagcacctt gtattatagc tttagattgc attacacata
    15721 tttcaggata agttattgca agtctacaac ctctgtgtcc aagcattggg ttaaactcat
    15781 ctaaatcaac tattctcttc ttaatatcac ttacttttat tcccatagat tttgaaagtt
    15841 cttctatagt ctcatcatca tgaggcaaaa attcatgtag tggtgggtct agtaatctta
    15901 tattaactgg ttttccatcc ataactttaa ataactctac aaaatcttct ctttgcattg
    15961 gtaatatttt ttcaagagct ttttctcttt gttctactgt attagataat atcatttctc
    16021 taactgctgg caatctagct tcatcaaaga acatatgctc agttctacat agacctatac
    16081 cttctgcacc aaactctata gctgcacgag catctctagg attatctgca ttagttctaa
    16141 ccatcataca tttgtgtttg tctacccagt tcattaattt ttcaaaattt ccagttaaag
    16201 atacttctgt tttcttaaca tctcctaggt atacatttcc tgtagaacca tctatagaaa
    16261 tgtattctcc ttcttttata actacatcat caagtgctct tatttcttta ttaaactcat
    16321 caacttttat ttctccacat ccagcaacac aacatttacc catacctcta gcaacaactg
    16381 ctgcatgtga tgtcattcca cctctagctg taaggattcc ttctgcttta accattcctt
    16441 ctatatcttc tggagatgtc tctagtctaa ctaaaactac tttttctcct ttttcatttg
    16501 cttttactac atcatcagca ttgaaataaa ctttaccact tgctgcacct ggtgatgctg
    16561 gtaaaccttt tgctataacc ttagcttctt ttaatgcctt atcttcaaat tttggatgta
    16621 ataattggtc taattgatta ggctcaattc tcatgatagc ttctttttca tcaattattc
    16681 cagcttcaac taaatcaacc gctacattta ttgctgctgc tgctgttctc ttaccatttc
    16741 tagtttgtag tatatataat ctttcatttt ctatagtaaa ctctatatct tgcatatctt
    16801 tataatgacc ttcaagtata tgagtaattt ttacaaattc atcgaataca gctggcatta
    16861 catctgcaag agttgatata ttttgaggag ttcttatacc tgcaacaacg tcttcacctt
    16921 gtgcattcat taaaaattca ccaaataatt tattttctcc tgtagctgga tttctagtaa
    16981 atgcaacacc tgtaccacta gtttctccca tattaccaaa taccattgat tgaatattta
    17041 ctgctgttcc taagttatga gctatatcat tcaatttacg gtatacgatt gcacgaggat
    17101 tattccatga tctaaataca gcctctattg caagcattaa ttgctctttt gggtcttgtg
    17161 ggaaatcatt ttttatttct tttttataaa ttgctttaaa ttcttttact atttctttta
    17221 aatcatcagt tgtaagttct gtatcaactg tgtatccctt tgcttctttt actctgtcta
    17281 atacattttc aaatttatat tttggaactt ccatagcaac atcagaaaac atttgtatga
    17341 atcttctgta actatcatat gcaaatcttt cattttgtgt agcctctgct aagccaacaa
    17401 cactattgtc attaagacct aggttaagaa ttgtatccat cattcctggc atagagaata
    17461 ctgcaccaga acgaactgat actaataatg gattcttatt gcatcctaat gttttattta
    17521 agtctttttc taaagtagct aaatgctctt ctatttcttt tattatctct gctcttatac
    17581 tttcattgtt aacataataa tcattacatg cttctgttgt tatagtgaat ccaggaggga
    17641 caggtaagcc tatcttagtc atttctgcta aattggcacc tttacctcct agtaaagatt
    17701 tcatgtcttt gcttccttca ccaaaactat aaacatactt agtttccatt gttaaatccc
    17761 ccattaatct ttttatttta atttatatca tttttattaa tagacattat taaatttcta
    17821 tttctttgta tatctttaga ccattttctt tcattatgtc taaaattata cctgctgttt
    17881 cttctattgc cttatttgaa acgtttatta ctgggcatcc aattcttttc attatctctt
    17941 ctgaataatc taactcctga agtattcttt ctaaattagc atagtttgct ttactggaca
    18001 atcctaaagc ttttaatctt tgggttctta tctcattcag cttttcaggt gaattagtaa
    18061 gacctattat ctttttagta tctatctcaa acacttcttt aggaattggt atttctggaa
    18121 ctaatggaac attagctact tttatatttt tattagctaa atacatactc aaaggagttt
    18181 tagatgttct tgatattccc actaaaatta tgtctgcttg aagaacacct ctagggtctt
    18241 ttccatcatc atatttcact gcaaattcta tagcttcaac tctcttaaag taagattcat
    18301 ctaattttct aattatacca gcttctcttt taggttttct tcctgttctc ttgcttattt
    18361 ctcttagtat atctgtcatt aaatctatac aactaagacc ttctctttcg caatatcttt
    18421 caacaatact gcataattct tcatcaacta atgtatatac aattaaagcg ttttcagctt
    18481 ttgcattctc caaagtttca agtagtactc ctttattaat catgtatgga aatcttttta
    18541 tttcataagt ttcattcaca taaaactgag acatacatgc ctttgcaact tgttgtgcag
    18601 tctcccctac tgaatctgat actgcatata taattaggtt tttcaccagc atacctcctt
    18661 aattgtcaac tatttctaag aatagcttag ttatgttggt ttttgatatt ctcccaacaa
    18721 ctctcatatg atttttgtct tctcccttat attcaacaat cggaatcgaa tctacttcat
    18781 gttctattat ttttcttgaa gctaataaaa cacttgctcc tttagttaaa gtgactacat
    18841 ttggagttct tgtcattatc atacctattg gcattttatt tatatcagaa cctcctatag
    18901 ttgcttttag caagtctttt ctggaaacaa ctccacataa ctcttcattc tcatctataa
    18961 taactatact ccctacatca gataaaaaca tagtgactat tacatcatat atactttcat
    19021 ccttcttagc tagtacaggc atgctcatta tatcctctac tgttttttct tttacacttt
    19081 taccaattaa atttatttca ctaactcctg aataaaaata tccaactttt ggtcttgcgt
    19141 ctaatattcc tgtcattgtt aatatagcta aatcagatct aagtgtagca cgagtcacat
    19201 tcaagctgga ggcaatactt tcactcgtta ttggttcatt ttccttaact atatctataa
    19261 tttttagttg tctttcatta agctgaataa taatcacctt ctttcattgt gctatacttt
    19321 ttatcttttc gcatcttata tagtatatta tataactata tataccattc tgtaaacaga
    19381 ttttttattg aatacgatat attttttact aagttctctc gcttaaagat atttattata
    19441 tattaatggg ggatatttct ttaattaata gaactatatt agtattacag ttaatattat
    19501 aatacataat tggtcatttt aattcatttt atattaaaat ttttaataat attatttcaa
    19561 tgtgaatata aaattgcaga actaattcaa aataattttt acgactacaa ataacctata
    19621 atatactttt aaagaaagta tcctaacaag aatatataaa aatttaaaag ggttgattta
    19681 aaaataacca tcaacccttt ttactaatat ctatttatat taccatcaaa tcttacccaa
    19741 atattttttc ttgcaattgt cttaaatgat ataaagtagt atctttttct atctctacca
    19801 tatcgtaagt tataacttca ccttttttaa tatcttttac aactttagtc tttttactta
    19861 tcaaaccaat aggtacagca ttcatagcct ttgctacatc gtatttttct atagtaccat
    19921 agcatgtata tgctccatag ccatccataa actcaccttt tttcaaatct ttcttagcta
    19981 tagtcataac ttctgctact agtccagctt taggtactat tgtagcctta tggtctaaaa
    20041 ctgccatagc tgctgataaa ggagtctcta aactacataa atgatatggt ctatataata
    20101 tgtaattagg tccatcaccc atacttaaat attttagttc atcattaact gcttttaatt
    20161 tatgggcaac tactacgaaa actccaggtg ctataccatt tacaaaatca actacaccat
    20221 aattatctaa tactccacct tcgcttttta atgcatattt cttaggtact tcattaactg
    20281 tagcttcaat accatgacca cctcttacat caggcacaaa tccagttgca ttacacatta
    20341 aagccatttc taccattgtt ttagttcctt ctttaaaaga agctatcata tgaggtgaag
    20401 ctccttttct ttctgcttct tctctaactg tatcaggatt acaatctaaa tctaatttat
    20461 tatttttacc tttacctatc actctaactt caaaccccat tgcatctgca aaatcaaata
    20521 attccattac tgcacctggc tcatctcctg cagaacctgt aaatattact ccagcatcat
    20581 ctgctaattt tttaagtatt ggtccaacaa cacaatcagt ttcagcattt aacataacta
    20641 tatgtttttt atttaaaata gagtttaatg ctatctctgc accaattgca atacctccag
    20701 ttgcatctat aactacatct attgacttag ttttacaagc cacaagaaaa tcattacatg
    20761 tcaatacttt tccttcttgt aattttattt ctgcatcctt ttcatttgtt acattatcta
    20821 taatttcttc atctattcct gcatcagtaa aagctttatg agccaactcc aaatttatat
    20881 ctacaacaat agaagcttca atacctttca tatttgccat ttggcttacc attccatttc
    20941 ccatatgccc agctcctata agagctactt ttattttttt accttcctct tccaattttg
    21001 caagttttct gtttaaatct aacataccaa ttcctcctgt tataataatt ttattttcaa
    21061 attaagaaat agttattaaa tctccaatag ttatatctgg atttcctatt tccacatgta
    21121 tttctcctgg taaatttact tcacattttc catcaaattt gattgtacaa tgcccaattt
    21181 ctctaagagt cttctgagca atatcaccaa ctgaaactat acaataatct ctattcccta
    21241 tagtaagtgt atctcctatt tcaacatcac tttttaacgt tgctattgaa tgaattacag
    21301 atatatcctt taagtcgtca tcattaatgg tttcatcata tataataaga caattgcttt
    21361 catccattaa ctctttaact aaactgccta tcccacttat cttaacttca tatttcatga
    21421 cattcctcct gataatttat atttatattg agttaattaa aagaaacatt tattttttct
    21481 tttataaatt ttatagcttg tacaatactc tcttcatctt taaatttttg ctctgcacta
    21541 tatatatcta atccttctat ttcttcaatc tctttaaatt tagccattac tgtacgccct
    21601 tttaaaattt ctccaaactt tataaaccca tctgaatctg aagatataat ggctatactt
    21661 cctttagaaa aatagctttt ctttttgcct gtagacacta tatcaccctt ttttttagct
    21721 ctatttaaag ttttaaaata caatttattc tgcttcatta taaatatagt atttaaaata
    21781 taagcaacaa ataaaatcac taataatttc atatttatac accttccaaa aatcacatta
    21841 tttttaaatc tgtataacaa ctataaatgc tgtacaatag ttatataagg tttttaagta
    21901 acaccttttt aaattctaaa aaagttctac atttacttat cttattcgtt aattttacat
    21961 cttggcctat ctttaaaact tcttcataaa tttttactaa taagtctgag tctatcttat
    22021 tctcacatgg aaacattgtc ataagaatta tttttatact tctatttgca tattctattg
    22081 gttcttctaa aattccaact gccattaaaa acttatctga tttttcattt acagaatgag
    22141 gaaataataa acctttatca aatactgtgg gagatttttc ttctctattg actatatttt
    22201 ttctaaactt atcatcaatg catcccaaat ccataagatg gtccatcatt ttttctaatg
    22261 aagcaagtat agtcttttca tttaatatca taaatttatc ttcatctaac aaattattta
    22321 ttagtaaatt tgtagaatta gtagtgtcaa tattcccttc tcttaggcac aatactgttt
    22381 ttaattgttc tcttagtttg ttttcatcaa atattgtatt tatttttaat actattgtat
    22441 ttatatttga tgtgtctaaa tcaatagttg taaaaactat atcatattca tctaagttaa
    22501 tatttctcaa attaaaactt gaaagagtat caattttagg attatttcct atcagttttt
    22561 ctactcttat tttcaatagt tttgctgttc ctagacctgt actacatata attgctattt
    22621 tttttattga atagaatcta ctgctatttc tttctaaata tccactgaaa tggattgcta
    22681 tatatccaat ctcatcttct gaaacttcta agttatactt tttctctatt acttttcctg
    22741 ctatttgtgc taatttaaat ggaagtatat aattttgctt aatctcttct aacaaaacat
    22801 tttttaactt tatattaaat aatagcctgt ttaaagtaaa actaagatga tatcctagac
    22861 ctactcttaa ttcatcctca tttataaaaa tacccatatc gacatatatt tgattaaata
    22921 tattttccat caactcatca atgtttttac taattctact actatttaaa gcacattctg
    22981 atgctggtgc atttccacta actaacggta tagttaaata aataatttct tcatcattta
    23041 aagtacattc aaacattcct gatattatat gttttatttc cctagcaata aagtattcat
    23101 catatgattt aagtaaatca aactttacat cataatcttt tactttcttt tcttctttta
    23161 ctctatagac taaaactact atgtagttaa taacttctct taacatttca tctgtaacat
    23221 aaaaattatt ttttttaaat aaagtcaata gtttttcttc taaatttatg atttcttctt
    23281 ttttgattcc tttaaaatac tttgaattac tgaaatcttt ccttgagttt ttatacaact
    23341 gatttaatat aaacagtcta atatcaagtt catttccatt taatttcata cctgtattct
    23401 gttttttaat caagtttaaa ttatatgaag ccaaaacttt ttctacatac tggaaatcat
    23461 ttactaaagt agttcttcct acacacatct catcagctaa actatcaagt gtaataaatc
    23521 catctagttc tatcaagtaa tttagtataa actctactct atcttctttt gaatttaatg
    23581 aaaatacatt ttttctattc atatcaataa tcttattgag tatctgctca tcttttattt
    23641 ctagtttata gccttgaccc tttatatatt tgattactgc tatatcatta cctaaatctt
    23701 tattcaattg ttttatatac gtccttacac ttctagttgt gacattcaat tcatttgcta
    23761 ttgagttaat actcacaaac tcttttttac tacacataat caatatctta aatatcttgc
    23821 ggtcaataga aggaattttc tcagttacac tcattttgta tctcctcctt gatttcattc
    23881 tagtacaaat ttgaaaccaa attaatacat aaaatttcac tttaataaag gaaaatttag
    23941 tattaatcta actcgataat tacagctaat taatagtata aaagctagtc tttcattaac
    24001 aaaaaatctt gactagctta aatttatatt tacaatttta ttaattaatt ccaagcctta
    24061 tattgacatt aaatattatc tcaatgcaaa tactgcaact ccatatgcaa taataacagc
    24121 tattggacca gttattaaac gagagtaaaa taaagctgga acaccatact ctacagtttc
    24181 tggttctgct tctcccaaac ttaatcctac tggtacaaaa tcacatccag cttgcccatt
    24241 tattgcaaat aaagctggaa gtgctaaata tgctggtatt gcaccaagac caatttgtgt
    24301 acctacaaga gtccctacta cttgagctat aactgcacca ggtcctaata ttggtgataa
    24361 gaatggaagt gtgcaaatta ctgatataat caataatcca ccaatattac ctgcaagtgg
    24421 agatataact cttgcaatcc agtctcccag tccagatgct aaaataattc ccataagcat
    24481 acttacaaat gccataaatg gtattacgtt attcattacc atttggatag tgtctcgacc
    24541 tgcatcataa aattttgata ctacttgccc tgccccttga ccaatagaaa gtattatgtt
    24601 ctttttagca tattcacctt gtgcttttga cttaacctca tcataattat caggctttct
    24661 aatagaactc ttattttcgc ttttattttc ttgagatttt tgtggcatat cttccccatc
    24721 tacaacactt atacagttag gattaacatc tgatacataa tattcttctg ttataaattt
    24781 tgctaaagga cctgtttttc caactggatt tacatttatt gtaggtattt ttttctttgg
    24841 ataaactcca catctagcag ttccaccaca atcaataata actactgcta cttcttcatc
    24901 agaaactcca gttttaaatc catctacaat ttcacaaccc agtagttctt ttatttttaa
    24961 tgttacttca tggataccac cagcagtcat tgatagtatc ttatttctat tcccttcttt
    25021 ttttatatac aaaggacctc cccagcctga agttcccttt tctattttta atattttatt
    25081 ctcctccatt ttatctacct cctaagcctt atttgttgct ctattttttc ttgttagaaa
    25141 agcatacatt ttttctgtta ctatacctct tataaaaatt acaattagac caactataaa
    25201 ataccacaat gccagtgtag tagtttcttt acctaaagta gtcaaacctg ctgatattcc
    25261 caaccataca aaaagttctc ctgcatttgc atgtggaaaa agacctgtaa ttggatgtac
    25321 aaatgaaact gctgcatcat aaaatcctgc tttgtgctct tcttttaaaa atctaccaaa
    25381 tgtatagcac ataggatttc ccaagaaaaa tactgatact aatggaagta gaatatatct
    25441 tgtaaaagag ttctttccta gcttagaagc ccaattatta actctttcct caccaattat
    25501 cttaatcaaa gagttaatag ctaccattaa tacaattagc gttggaagag tacctgaaac
    25561 aagacctata aattgttctc cccctttctg aaacaaaccg ataaaccact ctgcaccttt
    25621 acttagacct gtaataacgt tttccataaa attataacct ccctataata tctttaaatt
    25681 tttaatttta ctgcttacca taaattttac aaattacatc ttttttctgt actatatgct
    25741 aatattgtaa atgttttttt attaatatca ttttaatata atatttttga taaataaata
    25801 catctttttt caattttata aaggaaaaaa ttgtactaat tagaattttc agaaatatac
    25861 aattcttatt tcgactatat tctaaattct tcttagaaat ttaagaactt atttttattt
    25921 cttttattat taaaattatc taaatctgat aaaaataaaa aatagatgct cttttacgaa
    25981 catctatttc ttaatcaaat tatcttttag ttttcttata gattatttta taggtatata
    26041 aatatcaatt tgtgagttca cattactagg acctaaaaat cgttcatcat atacttcaaa
    26101 atcatctctt atatcaagtt cttcttttga acttaaaaac caagtacccc atatatattc
    26161 ataagttttg tctaaattat ctaagctacc agtatgagta aatatagcat actttccttc
    26221 cttaatagtt tttgaaacaa atgaatttgg aatcacatca taactattaa cttcaagtcc
    26281 tattatctca ttaaattcca tactttcact aactaggtgt atttcagaga ttgcttcaca
    26341 gattccaaaa actctctttg atggcaaagt atttgggaca atatgatgta cttttctgaa
    26401 tttctcccac aagtcaggta atgaattatt ttctaaaatt actttatcct ttatacctat
    26461 tacctttatt ttttctattt cttttataac tggtttaatc gttatatttc caacaagatg
    26521 tctcataaaa tctaactcta attttttctt tttaccaaca aatacatcaa ttctattttt
    26581 tctatattct atagggctaa ccttgtatat agatttaaat gctctgctaa atgcttccga
    26641 agattcaaat ccactaccaa tagctatatc tatcactttt ttatcactat ataaaagttc
    26701 ttttgtacta cttactaatc gtcttttttt tatataactt cctacactct caccaaacat
    26761 agacttaaat aagcgtgtta aatgataata agaatatcca gtttcttttg ccacatcatt
    26821 tacagttata ttttcatgta aattattctc aatatacatt acagcttttt caaaatactt
    26881 taagtaatcc aatatggcac ctctcttcta cttaaactct ttaaatatgt aaaatagcac
    26941 tattttttac tatactaaaa tattataact aattcaatac gataaataaa aacttttagt
    27001 tgatttatat gtcctaatta tttagaataa ttaaatacat tttttattca atgaaatcaa
    27061 aaaagtcaga tggtgctttt tcaatattta tgtttatcct ctgtgttata gatttaatgt
    27121 cattttttaa atagtagctc tcccatttat tctctagtcc accccataaa atattaaact
    27181 ttcctatatt taaatccata actatacttt ttaaagttcc aaaaaaatca ttatagtaat
    27241 tacaagataa gccatttgga tattttgaag acaataaatt cattaactct ttctctccaa
    27301 tttttttaga cttatttata tattctttaa ttaatttata tctatgaata gaatttttca
    27361 ttgattgtgg atctaattta tgaagtttat caatatgtgt atgattagta gagtgtaaaa
    27421 aatattctct tttactttct aaactgttga ttatatttac ttcttttttc ccatctaaag
    27481 tctccacaag tgcaatgttt ccagacttat ctgcaagcaa taaattaata ttgtatgcaa
    27541 ttggcatatc ttttaaatat tctaatgctt catctacatc tttgcaattt tctaacaaat
    27601 atctaataac agcccaaaac tgtaaaccta caattgctgg ttttcttaaa ccatctgaat
    27661 tgcctacagg aataccacat gatgactgac ttacacctaa gccacattca ttcatacctt
    27721 cacctcttcc aaattgcata atggtagtac ctatatgtgc atattttcca tttaccttag
    27781 ttttacataa aacaaaatcc tccatattgt gtgagaaatc gaaattacgt gcaaatagta
    27841 catgcccatt ttcagttaat tctggtgcta atgcaacttg actacaacct ggtttaagat
    27901 aagtcatagt ataatataaa atttgattac aattcacttg aattgcatca gcaaaacctt
    27961 gcaattcttc attaagacct ggacaatatt tatcaaacaa ctctatcatc tctttttctt
    28021 cttttttgct taccagatta gatttattaa tctgaccttc tattatctct ggcatattag
    28081 caaccaattc tcctagtctg tatcctatct cataatttgt accactcaat tcaatatttc
    28141 ttacttctaa agtttccata tgtacccctc ctatatatct tataatcgat tttatcattc
    28201 gaattgtaat cattcatgat attttgtgct acaagttaaa aaaggtcata taaaaacaga
    28261 taagcctctt tttcatgttt aaaataataa aaatatattt aatttcatac aaaaaagagt
    28321 gtctcataaa ctaaaaagtt cattttgaga cactcttctt attttaaaga gtataattaa
    28381 aaatagtcta agttctactc ttccttcatt tttttcaata attctggatt ttcaaataaa
    28441 ttaaatcctg ttaaagctaa tatttttata ccgtcaatta ctctatcttt tgcatatgaa
    28501 tttatagaag cctccaaaaa ttcttttgta tgtagttctt ctccattttc agaaaacttc
    28561 atcaaaggat gtatacatgg cattatttga cttaaatttc ccatatctgt tgaacctaac
    28621 attacttgtt catcttcata tattccaaat tcctccataa tctttgtaaa tatgtctacc
    28681 aaaactttat tttcatttaa tccttggcaa gttggttcaa agtgataaaa ttcaagttca
    28741 catccagtgg ctattgcagc acctctagca caatttttca ctttttctac tacttcatca
    28801 aagtaatttt gttcttttgc ccttatctct aatcttactt ttcccatatc aggtattaca
    28861 tttgcagcag ttcctgcttc taatataata ccatgtattc tcgttccatc tactaattgt
    28921 tgtcttagtg catttatatt attatatagt agtactactg catctaaagc atttattccg
    28981 ttatatggct tagctcctgc atgagatgct tttccagtaa atatgaaatc tagacctcct
    29041 attgctataa tttcaggtgt tacacaagtt tttgaatttg gatgtatcat cattgctgca
    29101 tcatagccat caaatacacc atgttctatc aagtaaggct ttccatctcc agtttcttca
    29161 gcaggtgttc caattatact tacttctccc ttgtaagtat ccagtatact tgaaagagct
    29221 attccagctc caactcccat agatgcaatt aaatgatgtc cacatgcatg tccattgcct
    29281 ggaagagcat catattctgc tataaatgct attttaggac catctccatt tttcttggtt
    29341 gctttaaaag ctgtagccat acctgccaag ttttcctcaa catcaaaacc ctcttctctt
    29401 agaaattctg atattttttt agcagattta tattcttgca aagcaatttc tggttcatca
    29461 tataaagatt gacatagccc tagaattttt tctttctttg aatcagccac ttgtattatt
    29521 ctttctttca taccaatctt acctcccact aaatttattt agtaattttt atattttatt
    29581 ataactttaa tttaactcat gcttatcctc ttttaagtat ctattttccc aaaactcagc
    29641 atttcttata tctaactcta tagggttaaa agttctatca ctccttttta ttcccatctt
    29701 cttctgtctt atataatcat aatatgcctt tatagctggt ttttttagtg taaagatggc
    29761 aattaaattt atatatgtta atacacctat acatatatca cctaatgccc agcttgtaag
    29821 tggttctata aaacaacaca ttgctgcaca tgctataagc ataaatctac agacattaat
    29881 tatgtacttt tcatgttttt ctgctttaat tctagttact aagtaagata cattagtctc
    29941 catcatatat gagaaggcta ataaagttgt aaatgtaaat aataataaaa ctattgctat
    30001 aaaaatatca cctaattgtg gatgtaatgt atttattgca ctctgtacat attgtggtcc
    30061 cggttctaca cctggcagtg ctgtgcaaac acctgtaaca ttatacattc ctgttattac
    30121 aatcataata cctgtagcca tacatattaa taatgtgtct acataaacag aaaatgcttg
    30181 aactaatcct tgtttggctg gatgacttac ttctgctgct gccgcaccat aagtcccaga
    30241 accttctcct gcttcatttg agtatatacc tctttttact ccccaagata ttgctgaacc
    30301 tagcattcct ccaaaaactg aaccaaatcc aaatgcagat ttaaacattg ttacaatagc
    30361 ctctggaact aaactaatat ttgttataat tattactaat gtcataataa ggtatgctcc
    30421 acccattata ggtactactt tttcagcaaa taaaccaatc cttttaattc ctccaaaaat
    30481 tattatcgct aatgcaatag aagatgctac tgctattgtc atttttgaaa atccaaatgc
    30541 ttgattcatt gctagatata aagtgtttga ttgtgtacca ggcataaaaa atgtataact
    30601 tattattgca actattgaaa ataaaactga acactttttc cacccaagac ctttttcaaa
    30661 ataatatgga ggtcctcctc tatactgccc atcatgctca tctttatata tttgagctag
    30721 agtagattct acaaatgcag ttgctgaacc taatgtagcc attatacaca tccatactaa
    30781 tgctcctgga cctcccattg ctattgctac agctacacca gctatatttc cagtccctat
    30841 tcttccagcc atagatgtag caaaaccttg aaatgatgat ataccaacat gagaactatc
    30901 tccttcaagc aagagagtaa acatttcttt tacatctctt atttgaaaaa attttaattt
    30961 caatgaaaaa tataatccta tacacaaaca cattgcaact aatacgggtg accaaacaat
    31021 tccattaatg gtgttaacta aatcggtcac ttaactcacc accattcatt ttatatttat
    31081 agacaataat ttaaacagct atatttcaat attttatata atctttatat taatatatct
    31141 aaaaaatcta ccttctattt atttatatta tctttaatct acaatctaca aatatttatt
    31201 tctaaaatca agagtattaa tcatatctaa acataaattt ataaaatttt taacgacgtg
    31261 tgtttgaata ataatc
//
LOCUS       DBGJFF010000033        30773 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.033, whole genome shotgun sequence.
ACCESSION   DBGJFF010000033 DBGJFF010000000
VERSION     DBGJFF010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30773
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.033"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            <1..780
                     /locus_tag="ACOIAF_001589"
     CDS             <1..780
                     /locus_tag="ACOIAF_001589"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HGZ2668603.1"
                     /translation="KIYYIFGYYDNALNTNKKLLEKSKNAKNYNYVKLAYKSIYLCFE
                     KIQNYEMSMKYFKMYYELKMMYIKARNRNYIDSLNYRHEYIEKEINNMRKVKLDLDKK
                     KYVDHLTSAYNRTFLNDFLEKQEVSEYCTAFMIDVDYFKEYNDTYGHYNGDIALKNIT
                     IIIKKYLKKDMLMIRYGGEEFLILSVCKDYKKSKIFGRKLCRIVKKFLNNNLTVSIGI
                     DTCKNSSIDIKEIIENADKALYKAKESGRNRCLHYHDFKNF"
     gene            864..2693
                     /locus_tag="ACOIAF_001590"
     CDS             864..2693
                     /locus_tag="ACOIAF_001590"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HGZ2668604.1"
                     /translation="MLTKFYLNLTDLNYILDYHKDLLNTKLEDNSRVYTLYLISLINY
                     IKKDREASYNYLIESLKYIKKLSLKDDSDIISKIYIYLTLSAISIRKYDKVNNFYFSA
                     KKIIRQNHLNELLVLLNMSLCVEISALYQTKTDVIALVEEVSFFKTLKNKALVSRANY
                     IFGRVYLYLFNNFIKSMEYLIECLKLAQENNLYSIEAFCKCIISLCYLESGNNVEAIK
                     YINNVLDVAHSNNSISVTERVSIKIDLIWAYLKEDMNKEAEVILLKTIKLMKVVEGVY
                     KDYLYSFIFLYSAEIELKKDNCSFEKVNTYLILSKNIYKSFGDVFIYNSKLDYIYKLY
                     GDLYIKFGNIDEGISCYIKGLNLVKKSSLNLRKISIFYGLIANGYELKKDFKLAIEYY
                     KSMDWYSDRWEKNNSYKTSQAIHKQYELRQKQESLRLLVDDNKKLENDILKDGLTKLY
                     NRRYLEQQLDLFNGTRDKNMVAILVDIDCFKSYNDNYGHLAGDKVIVKVTDIIRSVFI
                     DITEHIIRYGGEEILILVDSIESIGVEEIDNIELYIKRVFKLLEFEGIEHIYSKIKNY
                     ITISAGVSIKRCRSRKDVEMLIEDADKNLYKSKKAGRNQYIII"
     gene            2701..4701
                     /locus_tag="ACOIAF_001591"
     CDS             2701..4701
                     /locus_tag="ACOIAF_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HGZ2668605.1"
                     /translation="MILLRKKIGIIASDIELKERIEELYREDVENGTIIIDILNLDLM
                     ENQGRILVEKGAQAIIGRGGGYSLVIDTVNVPVIPMNMKSTDLLRAIEIAKKYSKKVV
                     LILGDNEVSFDYVGWRNVISTEITEEWFESKYEIRSKVVKYIDQKDEVVIVGGGLACS
                     FARQYGIDSVFATASDESIREAVEYCKKLLDTLGEEKFNNEVLRNILDGIKDGVIAID
                     SNGSIILYNESAKNMLKVERKCALNKYILDVFPKMEWMLDCLHEKEDVEDRKIRNINN
                     LIVNTRTTLIKVDNSTYGVLGIIQDITKLQNLERKIRFDLNQKGLYARYTFDDFLFKD
                     KLTKEFIEEAKKIGKSDYTTLLYGESGSGKEIIAHSIHNISKRKDRPFVAINCATIAE
                     NLLESELFGYEEGAFTGARKGGKRGLFELAHGGTLFLDEINSLSFNIQTKLLRVIEER
                     QIMRIGSDYIIPLDIRIIAATNESLTEKIVMGTFRADLFYRLSSLEINIPPLRDRRED
                     IIPLFNNFVNEVLKDDGLNGINSIDENFVLTKDEMDKLYNYSWPGNVRELKTIAQKYV
                     VTGKIKLRQDRDFKTKKLLPNSEVDKFNSETTASVEVQDESINISKINDGKISIDIKE
                     VNKYVEEKIISMLFAQGLSKNEVAQVLGISRTSLWKKYNKNI"
     gene            4963..6222
                     /locus_tag="ACOIAF_001592"
     CDS             4963..6222
                     /locus_tag="ACOIAF_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HGZ2668606.1"
                     /translation="MSQSMVGKHAMWPKENDVIFSISGRAQAAEKAFGMDNVINATIG
                     ALMDDSGKLITMKTVYEEYKALDNCEIGAYAALEGQPDYLEAVKKVFFRDYLPEGHIR
                     VLASPGGSGAIKLAVWNYTNEGDEVLTSDWFWSPYVSIAEEANRKVVNYQLFDENRRF
                     NFESFKEKFVNIAEKQGRVFTIINTPAHNPTGYSVADDEWDKILDLSKEVAKDKDKKI
                     IFFVDSAYIDFAGDDDVCRKFFKKFSNLPENVLVLVGFSMSKGFTAYGMRMGAIICIS
                     SSEDVAEEFHYSCVHSCRANWSNCNRSAMAVLSNIVNDPKKFKEYEDEKEIYKNMLTR
                     RADVFVKEAERVGLEILPYIAGFFVSIPCDNPKEVCEELTKHNLFAVPLKMGLRFAVC
                     AVSEDKCKKAPSIIKEALESLEVKINN"
     gene            6283..8070
                     /gene="iorA"
                     /locus_tag="ACOIAF_001593"
     CDS             6283..8070
                     /gene="iorA"
                     /locus_tag="ACOIAF_001593"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043805 - indolepyruvate ferredoxin
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HGZ2668607.1"
                     /translation="MKQLMTGNEAIARGAYEAGVKYASAYPGTPSTEILENIATYKDA
                     IVAEWAPNEKVALEAAIGGSIAGARTMASMKHVGLNVAADPIFTYAYTGVNGGMVLIT
                     ADEPGMHSSQNEQDNRMYAKFAKIPLFEPSTSQEAKDMIKEAFEVSEKYDTPVLYRVT
                     TRLCHSKGLVECYDREEVEIKEYVKNAKKMVTVPANAQIRRGVVEERMEILKKFSNET
                     DLNYYEINDTKIGVIASGMCCNFAKEVFGKNASYMKLGFTNPLPYEKIKEFAEKVDKI
                     YVIEENDPFIEEQIKAYGIDCIGKDVIPPYGEMTPDVLRKAIFGKTNDTIEYKSELVT
                     PRPPSFCAGCPHRGFFYELGKRKNLIVGGDIGCYTLGFAPPYNGIDYVVCMGSAFGTA
                     HGAQKVLNMKDDNEKRLVGVLGDSTFFHTGINGLLDVVYNRGNSISVILDNRITGMTG
                     HQENPGSGYTLQGAKTKEVDIEGLVKACGIEHVRVINPNNLKEVNEALDWALAIEDEP
                     SVIITRWPCVLKKFSKEDIEEFNNPFKTKCKVDHDKCIGCKLCLKTGCPALSFDKENK
                     LSNIDRNQCVGCGVCAQVCPKQAIVKEEK"
     gene            8072..8647
                     /locus_tag="ACOIAF_001594"
     CDS             8072..8647
                     /locus_tag="ACOIAF_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HGZ2668608.1"
                     /translation="MTKSILLVGVGGQGTILASKLLTMGLMEAGYDVKMSEIHGMSQR
                     GGSVSSQVRYGDCVHSPVIEIGGADILVSFEKMEALRWFNYLKPEGKALVNNHRIDSM
                     TVLIGGAEYQESDIDAELSRLNAKVINAADKAEELGNSKIMNVILLGCLVKSMQLESI
                     DWEKIISENVKPKFVELNIKAFHEGMEMVSK"
     gene            8700..9770
                     /gene="buk"
                     /locus_tag="ACOIAF_001595"
     CDS             8700..9770
                     /gene="buk"
                     /locus_tag="ACOIAF_001595"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HGZ2668609.1"
                     /translation="MTYRILAINPGSTSTKIAVYDGEEQILVKTIDHPAEEIAKYNTI
                     QDQFEMRKEAVLNILKENSIDLKSLSAIVGRGGVLPPVKSGAYLVNEEMIDVLRHRPV
                     LEHASNLGAVVAHAISEPLGINSYIYDSVAVDELIDVARISGLCGMDRSSAGHALNTR
                     AMALKYAKDKGKDYKSLNLIVAHIGGGVSIYLHEKGRMVDMLSDDEGPFSPERSGRVP
                     ATKLVAACYSGQYSEREMTKKIRGKGGIVSYLNTVDAREVEKMIAEGNEEAKIIYEAM
                     AYQLAKGIGELATVVDGKVDAIIITGGIAYSEMFTSMVKKKVEFIAPVEIMAGENELE
                     SLAFGTLRVLNGEEEARIYSEN"
     gene            9988..10689
                     /locus_tag="ACOIAF_001596"
     CDS             9988..10689
                     /locus_tag="ACOIAF_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HGZ2668610.1"
                     /translation="MGKISLKSMIRLFSGFFIFAISSVLMINAHVGLMPWDVLHQGLS
                     IKLGITIGQASIMVGVVIVILDAVFGENIGWGTLLNMTFIGIFIDLVIFSGVIPHASN
                     TYIGVFMVVIGIILAAIASFLYLGVCLGSGPRDGLMIALQKKTNKSVRLVRTILEILA
                     LVVGWLLGGSVGIGTLVSALGLGYVLQIVFRIFKFDTKLLKHRFIIDDIREWKEKKSN
                     EHKCKSSIVIKNEQN"
     gene            10749..11255
                     /locus_tag="ACOIAF_001597"
     CDS             10749..11255
                     /locus_tag="ACOIAF_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HGZ2668611.1"
                     /translation="MELWDLYNADGIKTGNVIKRGNSIEEGYYHLAVEVWILNSNSQI
                     LIQKRSKSKKTLPNMWGMTTGCIVSGEDSLEGAIREAKEEIGIDITKDEMKVFRSMIH
                     EDTLWDVYLVKKEYDISKAILQEEEVSDIKWVSTDEIRQLLKEGLFFEYPEIYELLYE
                     IDNNKLNI"
     gene            11430..12452
                     /locus_tag="ACOIAF_001598"
     CDS             11430..12452
                     /locus_tag="ACOIAF_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HGZ2668612.1"
                     /translation="MEDNKGLARDSSKYKIVSISVVVIILITFWYMLNMVLLTFIMTF
                     IFYNLLVATRKRIKKFSSLNIPDSLIIIVLYALFAILLVLISYAVVPIIIVQLTELSR
                     VFSDFDVNQFAQSLGPKLYPIVSKLDFNKYISQAGLLIASTATKVGSFGVNILLAFLL
                     SLLLLLEKNEIKNFGDKLSDSKISFIYNSLVFFGKSFVKNFGEVMKVQVMIAFINSVV
                     SMIFLGFMGFPQIWALGFMIFVLGLIPVAGVIVSLIPLTVIAFNTGGITKVFGVLLMI
                     CIVHAVETYILNPKLMSNRTKLPVCFVFIILLVGEHYLGVWGLLIGVPIFMFLMDILG
                     VKFTSR"
     gene            complement(12599..12907)
                     /locus_tag="ACOIAF_001599"
     CDS             complement(12599..12907)
                     /locus_tag="ACOIAF_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HGZ2668613.1"
                     /translation="MKNNNLNCAATNCAYNTSGYCYAGSIKVDGMQATTTENTYCASF
                     EDKYTSGITSRSNETNQVDTDNIHCEAVKCKYNKNELCKAEKVHINERNASCETFEMK
                     "
     gene            complement(12967..13395)
                     /locus_tag="ACOIAF_001600"
     CDS             complement(12967..13395)
                     /locus_tag="ACOIAF_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668614.1"
                     /translation="MNNKLHELEKNLPETSCSFCTHLSFKGPSLDYRYDIRCVISDTK
                     PDFRGCCEYFEPEYTELNTGDLDNLYINFLETCLKVNYNDYINSMYWKLFKERTLLEN
                     NFKCSICGSTENVDVYHVNKNLGRETSKDVVVKCDKCNCK"
     gene            complement(13618..15075)
                     /locus_tag="ACOIAF_001601"
     CDS             complement(13618..15075)
                     /locus_tag="ACOIAF_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HGZ2668615.1"
                     /translation="MTTFLIGLAILLIGGALYGAYCEKVFGPDDRKTPALAQSDGVDY
                     VPMKKWKNSLIELLNIAGTGPILGPIQGILFGPIAFILIPIGCVFGGALHDYMSGMIS
                     IREKGAQMPSLISRFLGNKVFQVYNIFLCLLMLLVGAVFIYTPGDLVVTQILNMKSTI
                     NNPVVWIVYGLIFLYYLCATLFPIDKIIGKVYPIFGAILLLSAAGVGIGIFTQGYDLA
                     NLSLANWKGIHPDGIPLIPTFFVTVACGIVSGFHSTQATLIARSVSNEKEGKTTFYNM
                     MILEGLIAMIWAAAAMGIYNKGIPKELVGSPDVIGLVARDLLGSIGGIIAIIGVIVLP
                     ITSGDTALRSLRLMLADYFHYDQKEKKHRVILSICIFIPVIAILIFAKLSASGFNILW
                     RYFSWSNQTIAIFAFAMITVYLIIKEKNYIISLIPGMFYSFVIFSYIFNAQIGFNLNI
                     NISYVLAAIFTVLYAILTVRSGRKLKSKADTKLAD"
     gene            15493..16407
                     /gene="nadA"
                     /locus_tag="ACOIAF_001602"
     CDS             15493..16407
                     /gene="nadA"
                     /locus_tag="ACOIAF_001602"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HGZ2668616.1"
                     /translation="MDKDLTYQIKELKKEKNAIILAHFYQPPEIQELADAVGDSYYLS
                     EIARDCKEEVVVFCGVRFMGESAKILSPEKTVLMPVSNAGCAMADMVDEEGVIKLKQQ
                     YPNALVVCYINSTAKVKAHCDVSVTSSSAIKILENIDNKEIIFLPDKNLGGYIAEQFP
                     DKNFIFWDGYCKYHNNIRAEEIIELKDKYKNAEVLVHPECKKEIRDLGDYVGSTSGII
                     KYATNSKNKDFIIATEEGILHELKKNNPNKNFYIPGGKILCTDMKKTTLENLYSTLKN
                     MENEVIVEDEIMEKALNSLLNMHKLAEG"
     gene            16420..17721
                     /locus_tag="ACOIAF_001603"
     CDS             16420..17721
                     /locus_tag="ACOIAF_001603"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HGZ2668617.1"
                     /translation="MNLEQDVLIVGSGVSGLYCALNLDKSLNVLVVSKSTIENNNTYL
                     AQGGISTARNIDDIESFVEDTMKAGQYKNRVEAVQVLADESIENVGQIVEYGMPLDKE
                     NGEIDYTREGAHSVNRIVHSKDNTGEVVFKTLLKEAKTRENITLIEDAYLLDILKDGN
                     KCIGARIFKSKKEIHVFSKIVVLATGGIGGLFKNSTNQRHLTGDGIAIALRNNIKIEN
                     LEYIQIHPTAFYEENNEGRRMLISESLRGEGAKLLNKNKERFVDELLPRDVVSKAIFE
                     QMEKDKLPYVYLDATHLDSEYLINRFSFIYNECLARGTDITKECIKVSPAQHYFMGGI
                     HVDLDSKTSMENLYAVGEISCTGVHGANRLASNSLLEGLVFSKRAAKNINSVIDNVKV
                     KFIDVPDMDIDIEQVKKENKILVIKEIERTSEDFGDELFDY"
     gene            17702..18538
                     /gene="nadC"
                     /locus_tag="ACOIAF_001604"
     CDS             17702..18538
                     /gene="nadC"
                     /locus_tag="ACOIAF_001604"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HGZ2668618.1"
                     /translation="MNYLIIDRMIQDALIEDVPSEDITTNSIVDENSKSTVDLICKQD
                     GIIAGLGVFKRVFEILGDVDVKLYKNDGDRVKNREKIAVLTGSTRNLLVGERVALNYL
                     QRMSGIATITSRYVEKLEGTNTKLLDSRKTIPNLRILDKYSVKVGGGCNHRFNLSDGI
                     LLKDNHIGAAGSVKKAVELARKNTSFVRKIEVEVETLDMVKEAIEANADIIMLDNMSL
                     EMAKQAVDIINGRAIVEFSGNVNLDTIEDIGKIGVDVVSVGALTHSVKALDISMKNLR
                     NI"
     gene            18794..19126
                     /gene="saoD"
                     /locus_tag="ACOIAF_001605"
     CDS             18794..19126
                     /gene="saoD"
                     /locus_tag="ACOIAF_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018942 - organometal metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE-related protein SaoD"
                     /protein_id="HGZ2668619.1"
                     /translation="MKVAYIFSSTNTHKILDKMIIPQLEQGTHGVDVLGMFFFMDNTL
                     FLSKGNPVGERLSKLHEKTDMIIMACDQCAIERGIENNLVDGATIGCFPNLYACLGSA
                     GVDQVITL"
     gene            19216..19473
                     /gene="saoT"
                     /locus_tag="ACOIAF_001606"
     CDS             19216..19473
                     /gene="saoT"
                     /locus_tag="ACOIAF_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_240067706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018942 - organometal metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:19249..19251,aa:Sec)
                     /transl_table=11
                     /product="thioredoxin-like (seleno)protein SaoT"
                     /protein_id="HGZ2668620.1"
                     /translation="MSKILVEFINTUPSCDEHGVFIKKMSEKYADKLEIKLYQAGKDF
                     SYIKKYGIITKGTLIINQKKKYDRLNKDTIERAIVEAINNN"
     gene            19500..20585
                     /gene="saoE"
                     /locus_tag="ACOIAF_001607"
     CDS             19500..20585
                     /gene="saoE"
                     /locus_tag="ACOIAF_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0018942 - organometal metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter SaoE"
                     /protein_id="HGZ2668621.1"
                     /translation="MNILEIVLNGVKDTIVASIVTLNGASGWLMISFILAGVLHNVLT
                     PRRFQKQLGNKKFSSLIKSTISGMFLPICSCGVIPLGISMYYSGAYLGPVLAFMTSTP
                     IINPIAVVLCLGLLGPKVTIIYLIAGFLVPFLVGFLGNCFGKEELSLHNDSEEEVIEL
                     EEEKRSFMGNMIDGLKWSFGELALTISKYVVMGMVFAGFITTIFPNSIIQKYLGNPGL
                     LSLGSIAILACVMYVCAVGHIPFIAALVASGASPGIAITFLIAGAATNLPELISMFKL
                     IGKRTVIIYSVTLTLSSIIIGYITNLLLVNNNSVNLDATNSSIQLANKLMLNIPDSFE
                     YVCTILIILFAFKSIIPKMKEVIGNHA"
     gene            20578..21036
                     /gene="saoC"
                     /locus_tag="ACOIAF_001608"
     CDS             20578..21036
                     /gene="saoC"
                     /locus_tag="ACOIAF_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018942 - organometal metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Cys-COOH (seleno)protein SaoC"
                     /protein_id="HGZ2668622.1"
                     /translation="MLKKIVIISCIVVVLIILSKIVDDNIKEDASIPNVNKETLEYFR
                     KNYKEDIITCAEEDLNNDGKKDLVVIYKKSNNSNEMVVVVSDKNSHYITKPIPAPIEN
                     QTITFKNIDDKAPIEVIVSGSKNGNVGYAIYRVEGKKFVDLFGEDMDKCC"
     gene            21065..22156
                     /gene="saoX"
                     /locus_tag="ACOIAF_001609"
     CDS             21065..22156
                     /gene="saoX"
                     /locus_tag="ACOIAF_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0018942 - organometal metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding subunit SaoX"
                     /protein_id="HGZ2668623.1"
                     /translation="MKLSKKIQALILFGVMGTSILTGCSSKPKEKEEAKTGKDDYTIT
                     VGYYNCDHMTAGPVAESAGIYKDLGLNVKTVGNGKVPEAMAAGKMDAGYIGTKGLVGA
                     IPKGSPITIAANNHTGGSEYLIVSKDIKEPKDLIGKKIATDMSDFLWTSDYGPETGLP
                     TDPSKYEVVNMDSDKDKYLALKTGKIQAFTSCDPWGSVAENDGAGKIIASTQYKEKAN
                     GKEYNCCSFSLNKNFIKEHPDLAKKLVLAHTKAIEYIYTNPAEAAKIFAKYYNVEEEV
                     ALRTIYKKTVGEGRTLTWKVTGEEYKNNLQMYKDLKALDDIPKYEDTIDTSLLDSCGA
                     DDFDKFIKEKVNPEFPEGMSYEDWKAKVL"
     gene            22170..22958
                     /gene="saoB"
                     /locus_tag="ACOIAF_001610"
     CDS             22170..22958
                     /gene="saoB"
                     /locus_tag="ACOIAF_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_239591066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0018942 - organometal metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:22383..22385,aa:Sec)
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     (seleno)protein SaoB"
                     /protein_id="HGZ2668624.1"
                     /translation="MKNIIILFIIILGSIFIIFFNFEDKEEIKGKLRIGVSDDTSGFV
                     INYMVNRDYFENIKVSDIMDIFTINDCUASTMQWALSSEEIDIAIICKDAAKQYVNVN
                     SGFEIVGTVVQNSDIFLLSDNNPEKIGVIQNRDYQSELVKKYYKNVEVAPLLGTALAY
                     GLESNLVDGVVIDCIKSIGLEGKRKSTTKLGDYDTYVLVVNKEFKKSKAYSDFITLYN
                     KSVDALKNKATFKKVLKEYRNIRNIDMEEISNWKLKFLKIKAID"
     gene            22982..23725
                     /gene="saoP"
                     /locus_tag="ACOIAF_001611"
     CDS             22982..23725
                     /gene="saoP"
                     /locus_tag="ACOIAF_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_239591060.1"
                     /note="Most members of this family are selenoproteins with
                     the selenocysteine residue at the channel-gating position;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0018942 - organometal metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:23546..23548,aa:Sec)
                     /transl_table=11
                     /product="ABC transporter permease subunit SaoP"
                     /protein_id="HGZ2668625.1"
                     /translation="MDILYKIILLVIIFLVWHFAAKDIGSSLLLPMPVDVIKGFFFCV
                     TDAETVTNLFITLQRVLKGFMYALLFGLPIGFIMGFSKTFERVLSPVVDSVRQVPIMA
                     WVPLTIVWFGIGDGPTIFLIAFSGVFPIILNTIQGVRAISKDYYNAARSMGASPIVIF
                     TNVIVPASLPDILTGSRIAISTGWMSVIUAEFIATSAGLGYSMVQAQTLMHTDVLIGL
                     MIFAALIGFIIDRVLKFINKILCRWRFAD"
     gene            23725..24477
                     /gene="saoA"
                     /locus_tag="ACOIAF_001612"
     CDS             23725..24477
                     /gene="saoA"
                     /locus_tag="ACOIAF_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0018942 - organometal metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein SaoA"
                     /protein_id="HGZ2668626.1"
                     /translation="MKLSVRDINKTFVNNRVHTKVLEDISMDIDDGQFVCLLGPSGCG
                     KTTLLTIIGGFQKSERGDVFINDKRVKKPGIDRAFIFQNYALFHWKTIRGNVLFPMKQ
                     QKIPKEKREEMLEELLVMSDLKGKEKLFPHQLSGGMKQRVAMIRALACNPEVLLMDEP
                     LGAVDFQMRQNLQEELERIWIKKKITALMVTHDVDEAVYMSDRVIVMSRDKGRIIDDI
                     NIDIPRPRIRGSQKYEEYKNKLTDTLSKCYEV"
     gene            24490..25131
                     /gene="saoL"
                     /locus_tag="ACOIAF_001613"
     CDS             24490..25131
                     /gene="saoL"
                     /locus_tag="ACOIAF_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_238476746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018942 - organometal metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:24973..24975,aa:Sec)
                     /transl_table=11
                     /product="MerB-like organometallic lyase SaoL"
                     /protein_id="HGZ2668627.1"
                     /translation="MSQIEKYNSLDNTQQKIRISIMDMIIDKGSSVTLQEVTEYVSKK
                     LNIEKEYIERTLQYFIYKNIMVVDGNSINFIYPVSALPTNHKVTLRDNRSFSAMCAID
                     AIGTSCTFNQDIKINSICSATGKEIEVIIKNEKIEYVNNPNLRVLHINLDKHLNWAAS
                     CUNIMNFFWTKSDFDAWTNEAGLSDDEDIYCLDINEAIVESYKIFKLKQKVLS"
     gene            complement(25268..26089)
                     /gene="yidA"
                     /locus_tag="ACOIAF_001614"
     CDS             complement(25268..26089)
                     /gene="yidA"
                     /locus_tag="ACOIAF_001614"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HGZ2668628.1"
                     /translation="MYKLIALDIDGTILNTQKRITPEVFESIQEAKRAGAKVVITTGR
                     PLPGVKELLNQLNLTDEGDYVICFNGAIIQEVKSEKIIHDVEMTLDDFDFIYNNVCKK
                     YKTKIHINTMTNLITPNETPGKYTLHEAKLNNIEVKYIQKDKIDESIKICKIMIVDEP
                     ERLEEIIQQLPKNLFNKYTIVRSAPFYLEFLGKTTNKGTALKTLCMNLNIPIENAIAV
                     GDEENDQHMIKYAGLGVAMGNARNSIKEIADYVTDTNNENGVAKVINKYILNKAI"
     gene            26582..28276
                     /locus_tag="ACOIAF_001615"
     CDS             26582..28276
                     /locus_tag="ACOIAF_001615"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HGZ2668629.1"
                     /translation="MKFSEFKYERPNYNSMKKEFLSCVEDINNSRNYKEQQKNIHKIN
                     LLRNKIETLSNIASIRYSTDTFNKFYKEEKNYWDEYMPLYEELNSYFYNAIVNSKFKY
                     DLIKEFGEQFFTIVEYSLKSFSKEIISELQEENKLCSEYTRLLASAEIMFDGKIRNLS
                     GMGKFMYSKNRKTRELANKAYYNFFEENETKFDDIFDKLVKLRDKMSKKLGFEDFVEL
                     GYVRMMRSDYREYMIKNVRKQVLKYVVPMANELYEKQAKRIGLEYLSYIDEGVEFLTG
                     NASLKGDSRYIIQNGKRMYSELSKESNEFFDFMLENELMDLETKKGKGAGGYCTYIPD
                     YKSPFIFSNFNQTADDIDVLTHEAGHAFQLYMSRWIDMPEINFPTLDSCEIHSMSMEF
                     ITWPWMDLFFKEDTDKYKFTHLSSSIKFIPYGVIVDEFQHYIYKNPNVDKSKRKEIWR
                     FLEKKYLPHRKYGDNSFLERGCWWFKQGHIFKNPFYYIDYVLAQICALQFWKKMIQDR
                     DAGWKDYINICKVGGTKSFLDIVSMGNLYSPFDDGCIESIIGDVKTWFDEINDSKL"
     regulatory      28608..28718
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            28822..29193
                     /locus_tag="ACOIAF_001616"
     CDS             28822..29193
                     /locus_tag="ACOIAF_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrdX family protein"
                     /protein_id="HGZ2668630.1"
                     /translation="MIIITNNPKVKEEVQGREVLFKDTTYIGILEASRDLIHEGYELL
                     SHPLYGSVKPNETPYRTVILKKGNRLDINSLTLIEEAIITASKFQNNKKTPKWTESVQ
                     DDFRVIDYDIFYNTIQRMQYE"
     gene            29289..30236
                     /gene="trxB"
                     /locus_tag="ACOIAF_001617"
     CDS             29289..30236
                     /gene="trxB"
                     /locus_tag="ACOIAF_001617"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HGZ2668631.1"
                     /translation="MENVYDLVIIGSGPAGLAAGLYGARAKLKTLILEKDKTGGQIVI
                     THEIANYPGSVPNATGPSLIARMVEQCKEFGAEMLRDNIVDTELDGDIKVLKGEKAEY
                     RAKAVIIGTGATPRKIGCPGEKELTGKGVSYCATCDADFFEDFEVFVVGGGDSALEEA
                     MYLTKFARKVTIVHRRQGFRCAKSVEEKAKANPKIEFLLDTVIEEIKGDGILESVVFK
                     NKVTGETHEYFADEEDGTMGVFVFVGLDAQTDLFKGKVHMDEKGYIITDEDMRTNIPG
                     VFAAGDCRSKTLRQVVTATNDGAIASIVAEKYIDEKFGN"
     gene            30321..30638
                     /locus_tag="ACOIAF_001618"
     CDS             30321..30638
                     /locus_tag="ACOIAF_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HGZ2668632.1"
                     /translation="MLDLDKATFEEEVLNAEGFVFVDFWSEGCEPCKALMPDVHKLAE
                     TYGDKIKFCKMDTTKARRLAIKQKVLGLPTMAIYKDGEKVDEVTKDDATVPNIENMIK
                     KYL"
BASE COUNT        12129 a         3186 c         5517 g         9941 t
ORIGIN      
        1 aagatatatt atatctttgg atattatgat aatgccttaa acacaaataa aaaactcctt
       61 gaaaaatcta aaaatgctaa aaattataat tatgtgaagt tagcatacaa gagtatatat
      121 ttgtgttttg aaaagattca aaattatgaa atgagtatga aatactttaa aatgtattat
      181 gagttaaaaa tgatgtatat aaaagctcga aatagaaatt atatagattc cttaaattat
      241 agacatgagt atatagaaaa agaaataaat aacatgagaa aagtaaaact tgatttagat
      301 aaaaaaaagt atgttgacca tttgacaagt gcatataata gaactttttt gaacgatttt
      361 ttagaaaaac aagaagttag tgagtattgc acagcattta tgatagatgt agattacttt
      421 aaagaataca atgatactta tggacactat aatggagata ttgctcttaa aaatataact
      481 ataataataa aaaaatatct aaaaaaagac atgcttatga taaggtatgg tggagaagaa
      541 tttctgatat tatctgtatg taaagattat aaaaaaagta aaatatttgg aagaaaatta
      601 tgtaggattg taaagaaatt tttaaacaat aatttgacag ttagtattgg cattgataca
      661 tgtaaaaata gtagtataga tataaaagaa ataattgaaa atgctgataa agctttatat
      721 aaagcaaagg aaagtggtag aaatagatgt ttgcattatc atgattttaa gaatttttaa
      781 ctgttgaaat aggattggtg atttacttga aaaggagttt aatagaagat ttaaagagaa
      841 atataaacaa gatagaatac gatatgttga ctaaattcta tctaaatttg acagatttaa
      901 actacatctt ggattatcac aaagaccttt taaatacaaa attagaagat aatagtagag
      961 tctatacttt atatttaatt agtcttataa actatataaa aaaagataga gaagcatcat
     1021 acaactattt gatagagtca ttaaagtata ttaaaaaatt gtcattaaaa gatgattcag
     1081 atataatatc taaaatttat atttatctta ctttatcagc tatttctata agaaaatatg
     1141 ataaggtaaa taatttttat ttctcagcaa aaaaaattat aagacaaaat catttaaatg
     1201 agttgttggt attgttaaat atgagtttat gtgtagaaat aagtgcttta tatcagacca
     1261 aaacggatgt aatagcttta gtagaagaag tgtctttttt taaaacttta aaaaataaag
     1321 ctcttgtaag tagggcaaat tatatatttg ggagagttta tctatattta tttaataact
     1381 ttataaaaag tatggaatac ttaattgaat gtctaaaatt ggctcaagaa aataatttat
     1441 actctataga ggctttttgt aaatgtataa taagtttgtg ttatttagag agtggaaata
     1501 atgtagaagc tataaaatac ataaataatg tattagatgt tgcacacagt aataactcaa
     1561 tatcagtaac tgaaagagtg tctataaaaa tagaccttat atgggcatat ttaaaagaag
     1621 atatgaataa agaagcagaa gttattttat taaagactat aaaattaatg aaagttgttg
     1681 aaggtgtata taaggattat ttatattcat ttatattttt atattcagct gaaattgagt
     1741 taaagaaaga taattgtagt tttgaaaagg taaacaccta tttaatttta tcaaaaaata
     1801 tatataaaag ttttggagat gtttttatat ataattctaa actagattac atatataagt
     1861 tgtatggaga tttatatata aaatttggta atatagatga aggtataagt tgttatataa
     1921 aaggtttaaa tttagttaaa aagagttcat tgaatcttag aaaaatatct atattttatg
     1981 ggctaatagc aaatggatat gagctaaaaa aagattttaa actggcaata gaatattata
     2041 aaagtatgga ttggtattct gataggtggg aaaaaaataa ttcctataaa acatctcaag
     2101 caattcacaa acaatatgaa ctcagacaaa aacaagagag tttaaggtta ctagtggatg
     2161 ataataagaa attagaaaat gatattttaa aagatggact tactaaacta tataatagaa
     2221 gatatctgga acagcagttg gatttattta atggaaccag agacaaaaat atggtcgcaa
     2281 tactggtcga tatagattgt tttaaaagtt ataatgataa ttatggtcat ttagcaggag
     2341 ataaagttat agttaaggta actgatatta taagaagtgt atttatagat attaccgaac
     2401 acataatcag atatggggga gaagaaattt taatattagt agattctata gaatcaatcg
     2461 gtgttgaaga gattgataat atagaattat atattaaaag agttttcaaa cttcttgaat
     2521 ttgagggtat tgaacatata tattctaaaa taaaaaacta tataactata agtgctggag
     2581 taagtataaa aaggtgtcgt agtagaaaag atgttgagat gttaattgaa gatgcagata
     2641 agaacctata taaatcaaaa aaagcaggta gaaatcaata cattataatt taaaattgag
     2701 gtgattttat tgcgaaaaaa aataggaatt atagcctctg atattgaatt aaaagaaaga
     2761 atagaagaat tatatagaga agatgtagaa aatggaacta taataatcga catacttaac
     2821 ctagacttaa tggaaaatca aggtcgtata cttgttgaaa aaggtgccca agctataatt
     2881 ggtagaggtg gaggttatag tttagtaata gatacagtaa atgtacctgt tataccaatg
     2941 aatatgaagt caactgacct tttaagagca atagaaatag caaagaaata ttctaaaaaa
     3001 gtagtattga ttcttggaga caatgaagtt tcttttgact atgttggctg gagaaatgtc
     3061 ataagtactg aaataactga agaatggttt gaatctaagt atgagataag aagtaaagtt
     3121 gtaaaatata tagaccaaaa agatgaagtt gtaatcgtag gaggaggttt ggcatgttct
     3181 tttgcaaggc aatatggaat tgatagtgta tttgcaactg ctagtgatga atctataaga
     3241 gaagctgtag aatattgtaa aaaattatta gatacattag gtgaagaaaa gtttaataat
     3301 gaagtgttac gaaatatact tgatggtata aaagatggag taattgctat agatagtaat
     3361 ggcagtataa ttttgtacaa tgaaagtgca aaaaacatgc ttaaagtaga gagaaaatgt
     3421 gcacttaata aatacatctt agatgtattt cctaaaatgg agtggatgtt agattgttta
     3481 catgaaaaag aggacgtaga agatagaaag ataagaaata taaataatct aattgttaat
     3541 actaggacaa cattgataaa agttgataat agtacttatg gagttttagg aataatacaa
     3601 gatataacca agttacaaaa cttagaaaga aaaataagat ttgacttgaa tcaaaaaggt
     3661 ctctatgcta gatatacttt tgatgatttt ttatttaaag ataaattaac taaagaattt
     3721 attgaagaag ctaaaaaaat tggaaaaagt gattatacaa ctttacttta tggagaaagt
     3781 gggtctggta aagaaattat agctcatagt atacacaata taagtaaaag aaaagatagg
     3841 ccttttgttg ccataaattg tgctactata gcagaaaatt tacttgaaag tgaacttttt
     3901 ggctatgaag aaggtgcatt tactggtgct agaaagggtg gaaaaagagg gctatttgaa
     3961 cttgctcatg gagggactct ttttttagat gaaataaata gtctatcctt taatattcag
     4021 acaaaactat taagagtaat agaggaacgt caaataatga gaattggttc tgattatata
     4081 atacctctgg atataaggat tatagcagca acgaacgaat ctttaaccga aaaaatagtt
     4141 atgggtacat ttagagcaga cttattttat agattgagta gtttagagat aaatatacct
     4201 cctttaagag acagaagaga agatataata cctttgttta acaattttgt aaatgaagta
     4261 ttaaaggatg atggtctcaa tggaataaac agtatagatg aaaattttgt tttgactaaa
     4321 gatgaaatgg ataagctata taattatagc tggcctggaa atgtaagaga gttaaagaca
     4381 atagctcaaa aatatgttgt gacaggtaaa attaaattga gacaagatag agattttaaa
     4441 actaaaaaat tacttcctaa ctctgaagta gacaaattta attctgaaac gacagcaagt
     4501 gtagaagtcc aagatgaatc tataaatata agtaagataa atgatggtaa gataagtata
     4561 gatataaaag aagtaaataa gtatgttgaa gaaaaaataa taagtatgct ttttgctcag
     4621 ggactttcta aaaatgaagt tgcacaagtt cttggaataa gcaggacatc tttatggaag
     4681 aaatacaata aaaatattta aatataatta catataaatt attttgaagt aatgttaatt
     4741 ttttaaaaca agtgttaaga aaaattaaca ttactttttt gcaaattttt atttttttag
     4801 acaggcatat atatttataa gaaaaatagt taaaacactt aatttaataa tgaaaaaaat
     4861 aataattcta aacaaaatca attttacata aagtgggatt tattggcata gtaattgctt
     4921 aatatataat gtaagagtaa atacaatttt aggggtgatg gtatgagtca aagtatggtt
     4981 ggtaagcatg caatgtggcc aaaagaaaat gatgttatat ttagcatatc tggcagagct
     5041 caagcagcag agaaggcttt tggtatggat aatgtaataa atgcaacaat aggcgctcta
     5101 atggatgatt caggaaaatt gataacaatg aaaactgtat atgaagaata taaagcatta
     5161 gataactgtg agataggtgc atatgcagct cttgaaggtc aaccagatta cttagaagca
     5221 gttaaaaaag tatttttcag agattactta ccagaaggac atataagagt attagcttca
     5281 ccaggaggaa gtggtgcaat aaaattagct gtatggaact atactaatga aggagatgag
     5341 gtattaacat ctgattggtt ctggagtcca tatgtaagta tagctgaaga ggctaataga
     5401 aaagtagtta attaccaact ttttgatgaa aatagaaggt ttaattttga gtcgtttaaa
     5461 gaaaaatttg taaatatagc agaaaaacaa ggtagagtct ttacaattat aaacacacca
     5521 gcgcataatc caactggata tagtgttgca gatgatgagt gggataaaat attagactta
     5581 tcaaaagaag tcgcaaaaga taaagacaaa aagataatat ttttcgtaga ttcagcatat
     5641 attgactttg caggtgatga tgatgtatgt agaaagtttt ttaagaaatt ttctaatttg
     5701 cctgaaaacg ttctcgtatt ggttggattc agtatgtcaa aaggatttac agcttatggt
     5761 atgagaatgg gggctataat ttgtatatct tcaagtgaag atgtagctga agagttccat
     5821 tactcatgtg ttcattcatg tagagcaaat tggtcaaatt gtaatagaag tgccatggca
     5881 gtattatcta acattgtaaa tgaccctaaa aaattcaaag aatatgagga tgaaaaagaa
     5941 atatacaaaa acatgcttac tagaagagca gatgtatttg taaaagaagc tgaaagagta
     6001 ggattagaaa ttcttccata tatagcaggt ttctttgtaa gtataccatg tgataatcca
     6061 aaagaagtat gtgaagaatt gactaaacat aatttatttg cagttccatt aaaaatgggc
     6121 ttaagatttg cagtttgtgc agtttctgaa gataaatgta agaaagcacc aagtattata
     6181 aaagaagcac ttgaaagttt agaagtaaaa ataaataatt aatttcattg aacaaattgg
     6241 gtggactatg aatttaataa ttgtagatgg agggaatttt atatgaaaca attgatgaca
     6301 ggtaatgaag caattgcacg tggagcctat gaggctggtg ttaaatatgc atctgcatat
     6361 ccagggactc caagtacaga aatattagaa aatatagcaa catataaaga tgcaatagtt
     6421 gcagagtggg ctccaaacga aaaggtagca cttgaggctg ctataggggg ttcaattgca
     6481 ggagcaagaa caatggcttc tatgaaacat gttggtttga atgttgcagc tgatccaata
     6541 tttacttatg catatacagg ggtaaatggt ggtatggtac ttattacagc agatgagccg
     6601 ggaatgcatt catctcaaaa tgagcaagac aataggatgt atgcaaaatt tgcaaagata
     6661 cctttatttg aaccatcaac tagtcaagaa gctaaagata tgataaaaga agcttttgaa
     6721 gttagtgaaa aatacgatac accagtttta tatagagtta caacaagact ttgtcattca
     6781 aaaggattag tggagtgcta tgatagagaa gaagttgaaa ttaaagagta tgttaaaaat
     6841 gctaagaaaa tggtaactgt tccagcaaat gctcaaataa gaaggggcgt tgttgaagaa
     6901 agaatggaaa tccttaaaaa attctcaaat gaaactgatt taaactacta tgaaataaat
     6961 gatacaaaaa taggtgttat agcctctggt atgtgttgta attttgccaa agaagttttt
     7021 ggaaaaaatg catcatatat gaaactagga tttacaaatc cattacctta tgaaaaaata
     7081 aaagaatttg cagaaaaagt tgataaaata tatgttatag aagaaaatga cccatttata
     7141 gaagaacaaa taaaagcata tggaatagat tgtattggaa aagatgtaat accaccatat
     7201 ggagaaatga caccagatgt tttaagaaaa gctatatttg gaaaaacaaa tgatactata
     7261 gaatacaaat ctgaattagt aacaccaaga ccaccaagtt tctgtgcagg atgtccacat
     7321 agaggattct tctatgaact tggaaagaga aaaaatttaa tagttggtgg agatataggt
     7381 tgttacactc ttggatttgc tcctccatat aatggaatag attatgttgt ttgtatgggt
     7441 tctgcttttg gaactgcaca tggagctcaa aaagttctta atatgaagga tgataatgaa
     7501 aaaagattgg taggtgtact tggagactca acattcttcc atacaggaat aaatggactt
     7561 ttagatgttg tttacaatag aggtaattct atttcggtta tattagataa tagaataact
     7621 ggaatgacag gtcaccaaga aaacccaggt tcaggatata ctttacaagg tgctaaaact
     7681 aaagaagttg atatagaagg attagttaaa gcttgtggaa tagaacatgt aagagttata
     7741 aatcctaaca atttaaaaga agtaaatgaa gcactagatt gggctttagc tatagaagat
     7801 gaaccatcag taataataac aagatggcca tgtgtactta aaaaattctc taaagaagat
     7861 atagaagaat ttaataatcc atttaagact aagtgtaaag ttgaccatga taaatgtata
     7921 ggatgtaaat tatgtttgaa aactggatgt ccagctctat catttgataa agagaataag
     7981 ttatcaaata tagatagaaa tcagtgtgta ggatgtggag tatgtgcaca agtttgtcca
     8041 aaacaagcaa tagttaagga ggaaaaataa tatgactaag agtatacttt tggtaggtgt
     8101 aggaggtcaa ggaactatac ttgctagtaa attattaact atgggattga tggaagctgg
     8161 atatgatgta aagatgagtg aaatacatgg aatgagtcaa agaggaggtt cagtttcctc
     8221 acaagttaga tatggagatt gtgttcattc tccagttata gaaattggtg gagcagatat
     8281 attagtatct tttgaaaaaa tggaagcttt aagatggttc aattatttaa aaccagaagg
     8341 aaaagcttta gtgaataatc ataggataga ttctatgact gttttaatcg gaggagctga
     8401 atatcaagaa agtgatatag atgcagagtt aagtagatta aatgcaaaag ttataaatgc
     8461 agctgataaa gctgaagaac taggaaattc aaaaataatg aatgttattt tattaggatg
     8521 tctagttaaa tctatgcaat tagagtctat tgattgggaa aaaattataa gtgaaaatgt
     8581 taaaccaaaa tttgttgaat taaacataaa agctttccat gaaggtatgg aaatggttag
     8641 caaatagttt ataatagata taaatagact atagaaatta aggtgaaagg aagaaaatta
     8701 tgacttacag aatattagcc ataaatccag gttctacttc tacaaaaata gcagtatatg
     8761 atggagaaga acaaattctt gtgaagacga tagaccatcc ggctgaagag attgcaaaat
     8821 ataatactat acaagaccag tttgaaatgc gtaaggaagc agttttgaat attcttaaag
     8881 aaaatagtat agacttaaaa tctcttagtg caatagtagg aagaggtgga gttttaccac
     8941 cagtaaaatc aggagcatat ttagtaaatg aagaaatgat tgatgtacta agacatagac
     9001 cagtacttga acacgcttcc aatttaggtg ctgttgtggc acatgcaata tcagaacctc
     9061 ttggaatcaa ctcatatatt tatgattctg ttgcagtaga tgagcttata gatgtagcga
     9121 gaatatctgg actttgtgga atggatagat caagtgcagg gcatgcatta aatactagag
     9181 caatggcttt aaaatatgct aaggataaag gaaaagatta taagagctta aacttaatag
     9241 tagctcacat tggtggagga gtaagtattt atcttcatga aaaaggaaga atggttgata
     9301 tgctatctga tgatgaagga ccattttctc cagaaaggtc aggaagagta cctgctacaa
     9361 aattagtggc tgcctgttat tcaggtcaat attcagaaag agaaatgact aaaaagataa
     9421 gaggtaaagg tggtatagtt tcatacctaa atactgtaga tgctagagaa gttgaaaaaa
     9481 tgatagcaga aggaaatgaa gaagcaaaaa ttatttatga agcaatggct tatcagttag
     9541 caaaaggtat tggagagtta gcaactgtag tagatggaaa ggtagatgct ataattataa
     9601 caggtggaat tgcatattct gaaatgttta cttcaatggt taaaaagaaa gttgagttta
     9661 tagcaccagt agaaattatg gcaggagaaa atgagttgga atcacttgct tttggaactt
     9721 taagagtact aaatggagaa gaagaagcta gaatttatag tgaaaattaa taagaaaaac
     9781 taaaaatgtt tatatactaa aaaagtggta tatccgtttt ttatacacga aagcttagag
     9841 tataggtgaa aatgtataaa atttggattg ccacttttta taattaaaat tttatcttaa
     9901 cctaataaat aattctgtaa aatttcgtat ttatatatat attttattgg tatttattgt
     9961 tattattatt atgaggagtg atttttgatg ggaaaaataa gtttaaaaag tatgataaga
    10021 ttattttcag gtttttttat atttgcaatt tcttctgtgc ttatgattaa tgcacatgta
    10081 ggtcttatgc cttgggatgt acttcaccaa ggtttatcta taaagctagg cataactata
    10141 ggtcaggctt ctataatggt aggagttgta attgttatat tggatgcagt ttttggtgaa
    10201 aatataggat ggggtacttt actcaacatg acatttatag gaatttttat tgatttagta
    10261 atattttctg gagtgatacc tcatgcaagc aacacttata taggtgtgtt tatggttgtt
    10321 ataggaatta ttttggcagc tatagcaagt tttctatact taggtgtttg tttaggaagt
    10381 ggaccgagag atgggcttat gattgctctt cagaaaaaaa ctaataaatc tgtacgtctt
    10441 gttagaacaa ttcttgaaat actagcatta gttgttgggt ggttattagg aggttctgta
    10501 ggtattggta cgcttgtaag tgctttaggt ttaggatatg tattacaaat agtgtttaga
    10561 atatttaaat ttgacacaaa attgttgaaa catagattta ttatagatga tataagagaa
    10621 tggaaggaaa agaaaagcaa tgaacataaa tgtaaatcaa gtattgtaat aaaaaatgag
    10681 cagaattaat tatgctaaaa tatatttgta gtttatctaa tttatagtaa agtcaaggag
    10741 attttgaaat ggaattatgg gatttatata atgctgatgg aattaaaaca ggaaatgtta
    10801 taaaaagagg taattctatt gaagaaggtt attaccattt ggcagttgaa gtgtggattt
    10861 taaatagtaa ctctcaaatt ttaattcaga aaaggtctaa aagtaaaaag acattgccaa
    10921 atatgtgggg aatgacaact ggatgtattg tttctgggga agacagttta gaaggagcaa
    10981 ttcgcgaagc aaaggaagaa atagggatag atataactaa agatgagatg aaagttttta
    11041 gaagtatgat tcatgaagat actctttggg atgtatactt agttaaaaaa gaatatgata
    11101 tatccaaagc tattttacag gaggaagaag taagtgatat aaagtgggta agcacagatg
    11161 aaataagaca attactaaaa gagggtttgt tttttgaata tccagagata tacgaattgc
    11221 tatatgagat agataataat aaattaaata tatgatgtag agcagatttc agtataagta
    11281 agattattta aaatgtatct tctctaaatt ttaatattgt tttatgaaaa ttaataagtt
    11341 atttttagta tttttctact tgttttttga gattaaaata aaaagggcag tcattataaa
    11401 gtcataaaaa tttggggagg atttaaacta tggaagataa caaaggattg gcacgtgaca
    11461 gttcaaaata taaaattgtt tctatatctg tcgtagtaat tattctaatt acattttggt
    11521 atatgttaaa tatggtgctt ttaacattta ttatgacttt tattttctat aacttgcttg
    11581 tagccacaag aaaacgaatt aagaagtttt cttctttaaa tattccagat tcattgataa
    11641 ttatagtgtt atatgcacta tttgctatat tgttagtatt gattagctat gcagttgtac
    11701 caataattat agttcaactt acagagttga gtagggtttt tagtgatttt gatgtaaatc
    11761 aatttgctca atctttagga ccaaaattgt atcctattgt ctcaaagctg gattttaata
    11821 aatatatttc acaagcaggg ctacttatag catcaacagc aacaaaagtt ggaagttttg
    11881 gagtaaatat attattggca tttttactga gtttactcct tttacttgaa aaaaatgaaa
    11941 ttaaaaattt tggagataaa ctttctgata gtaagatttc ttttatttat aattcgcttg
    12001 tattttttgg aaagagcttt gttaaaaatt ttggtgaagt aatgaaagta caagttatga
    12061 ttgcctttat aaattctgtt gtttctatga ttttcttagg tttcatggga ttccctcaaa
    12121 tatgggcttt aggatttatg atttttgtgt tgggtcttat tccagtagca ggagttatag
    12181 tgtctttgat tccattaact gtaatagcat ttaatacagg aggaataaca aaggtttttg
    12241 gagttttact tatgatttgt atagtgcatg cagtagaaac atacatatta aaccctaagt
    12301 tgatgtcgaa tagaactaaa cttccagttt gttttgtttt tattatttta cttgtaggag
    12361 agcattattt aggtgtatgg ggattactaa ttggagttcc aatatttatg ttccttatgg
    12421 atattctggg tgttaaattt acttctaggt aatatattaa aataataatt tatttttata
    12481 tataaattat gaattgtttt aaaaaagaac ctataaatag acatcattaa taaagacatc
    12541 tatttgatag gttcaatttt gctttgtata aatactataa aactaaataa aaagaaattt
    12601 atttcatttc aaaagtttca caacttgcat ttctttcatt tatatgaact ttttcagctt
    12661 tgcaaagttc atttttattg tatttacatt ttacagcttc acaatgaatg ttatctgtat
    12721 caacttggtt agtttcatta gaacgactag ttataccaga tgtatatttg tcttcaaaag
    12781 aagcacaata agtattttct gtagtagttg cttgcattcc atctactttt atactaccag
    12841 cataacaata tccactagta ttataagcac aattagtagc agcacaattt aaattattat
    12901 ttttcataaa taacctccta agttttattt aaacttatta tgtgaagtta tctaaatttt
    12961 atacacttat ttacaattac atttatcaca tttaacaact acatctttgg aagtttccct
    13021 tcctaaattt ttattaacat gatacacatc aacattttca gttgatccac atatagaaca
    13081 tttaaaattg ttttctagta gggttctttc tttaaataac ttccaataca tagaattgat
    13141 gtaatcatta taatttacct tgaggcatgt ctcaagaaag tttatgtata gattgtctaa
    13201 atctcctgta tttaattctg tatattctgg ttcaaaatat tcacaacatc ctctaaaatc
    13261 gggctttgta tctgaaataa cacatctaat atcatatcta tagtctaggc taggcccctt
    13321 aaatgaaaga tgtgtgcaaa aagaacaaga tgtttctggt agattttttt caagttcatg
    13381 taatttatta ttcataagta ttcctccaaa aaatttattt taagtatata aacgaataga
    13441 ttattttaaa acttttattt atttatatta gagatttagg ataaatattc caataaggag
    13501 taaatactta agtatttatt actgtattaa ataaaaaaga ggcaaatcta acttgagatt
    13561 gcctactttt ttatcatttt ttaataaaat ttattctaag tattatttta tttagattta
    13621 gtcagccaac ttagtatctg ctttgctttt taactttcta ccagagcgta cagttaaaat
    13681 agcatataat acagtaaata tggcagctaa aacatatgat atatttatgt ttaagttaaa
    13741 tccaatttga gcattaaata tataactgaa tataacaaag gaataaaaca taccaggaat
    13801 taagcttata atataattct tttcttttat tataaggtaa actgtaatca ttgcaaatgc
    13861 aaatatggca atagtttgat tactccaaga gaagtatctc cacaatatat taaatccaga
    13921 agcactaagc tttgcaaata taaggatggc tatgacagga ataaatatac agattgacaa
    13981 tataacacga tgtttttttt ccttttggtc ataatgaaag taatctgcaa gcattaatct
    14041 taaagatctt aaagctgtat ctccagaagt tattggtaaa acaattacac ctattatagc
    14101 tatgatacca ccaatagaac caagtaaatc tcttgcaacc aaacctatta catcaggaga
    14161 acctactaat tccttaggta ttcctttatt gtaaataccc atagcagctg cagcccaaat
    14221 cattgcaatt aaaccttcta aaatcatcat attataaaaa gtagtttttc cttctttttc
    14281 atttgaaact gatcttgcta taagagtagc ttgagttgag tggaatccag aaacaatacc
    14341 acaagcaact gtaacaaaga atgtaggaat aagaggaata ccatcaggat gaataccttt
    14401 ccaatttgca agacttaaat tagctaaatc ataaccttgt gtaaagattc caattccaac
    14461 accagcagca gataaaagta gaatagctcc aaatattgga tatacctttc ctatgatttt
    14521 atcaattgga aatagtgttg cacacaagta atatagaaag ataagtccat aaactatcca
    14581 gacaactgga ttatttatag tcgatttcat atttaaaatt tgagtaacaa ctaaatcccc
    14641 aggtgtataa ataaaaactg caccaactaa aagcattaaa agacaaagga atatattata
    14701 gacttgaaat actttattac ctaaaaatct actgataaga gaaggcattt gtgctccttt
    14761 ttctcttata ctaatcattc cactcatata gtcatgcaaa gctcctccaa atacacagcc
    14821 aataggtatt agtatgaatg ctattggacc aaacaatata ccttggatag gtcctagaat
    14881 tggtcctgta ccagctatat taagcaactc aattagacta tttttccatt ttttcattgg
    14941 gacatagtct actccatcac tttgagcaag tgcaggtgtt tttctgtcat ctgggccaaa
    15001 tactttttca caataagcac cgtataaagc acctcctatt agtaatatag ccaagccgat
    15061 taagaatgtt gtcataatat caccccattt ataaaatttt ttggtctttg tatacatatt
    15121 atctaaatgt gtagatttat attgaattta attctggaca acctacaaat tctataaagt
    15181 attacaacta atataatttc agaaataaat acatatacat aaccatgtac ttagtataca
    15241 ggtatttggt aatattttca atgttatagc aataaagaat ataaacaaaa ttctgataat
    15301 attttacaaa aaaattaaaa aatataaaaa ttagtttaaa tagatggaga aaaaaggaaa
    15361 atcaattgag aatataaatg tctatatctt aacaatatta agaggtttaa aattcttgaa
    15421 gaaagaatag tcaagtgata taatgagttt acaggtgtat atacactagt acatatagaa
    15481 agcggtgcaa ttatggataa agatttaaca tatcaaatca aagagctaaa gaaagaaaaa
    15541 aatgcaataa tacttgccca tttttatcaa ccaccagaaa tacaagaatt agcagatgca
    15601 gttggtgact catattattt aagtgagatt gctagagatt gtaaagaaga agtggttgta
    15661 ttttgtggag taaggtttat gggagaaagt gctaagatat tatcacctga aaaaactgta
    15721 ttgatgccag taagtaacgc tgggtgtgct atggcagata tggtagatga agaaggcgtg
    15781 ataaaattaa aacaacagta tccaaatgct ttggttgtat gttatataaa ctctacagct
    15841 aaagttaagg cacattgtga tgtttctgtt acatcttcta gtgcaattaa aatactagaa
    15901 aacatagata ataaagaaat tatattcctt ccagataaaa atttaggagg atatattgca
    15961 gaacaatttc cagataaaaa ctttatattt tgggacggat attgtaaata ccataacaat
    16021 ataagagctg aagaaataat tgagttaaaa gataagtata aaaatgcaga agtattagta
    16081 catccagaat gtaaaaaaga aataagagat ttaggagatt atgtaggtag tacatcagga
    16141 ataataaaat atgctactaa tagtaaaaac aaagacttta taatagcaac tgaagaaggt
    16201 atattacatg aattaaagaa aaataatcct aataaaaact tttatattcc tggtggaaaa
    16261 atactatgta cagatatgaa aaagacaaca ttagaaaatt tatatagtac acttaaaaat
    16321 atggaaaatg aagttattgt agaagatgaa atcatggaaa aggctttaaa ttcactgctt
    16381 aacatgcaca aattagcaga aggatagagg gatagactta tgaatttaga acaagatgtt
    16441 ttaatagttg gaagtggagt ttcaggatta tactgtgcat taaatttaga taaaagttta
    16501 aatgtgctag tagtatcgaa atcaacaatt gaaaacaaca atacatatct tgcacaaggt
    16561 ggaatttcaa ctgctagaaa tattgatgat atagaatcct ttgtagaaga tacaatgaag
    16621 gctgggcagt ataaaaatag agttgaagca gttcaagtac ttgcagatga atctatagaa
    16681 aatgtagggc agatagtaga atatggtatg cctttagaca aagaaaatgg agagatagat
    16741 tacacaagag aaggagcaca tagtgtaaat cgaatagttc atagtaagga taatactgga
    16801 gaagttgttt ttaaaacttt attaaaagaa gctaaaacta gagaaaatat aactcttata
    16861 gaagatgcct atcttttaga tatattaaaa gatggcaata aatgtatagg agctagaatt
    16921 tttaaatcta aaaaagagat tcatgttttt tcaaaaatag tagttttagc tacaggtgga
    16981 attggtgggc tttttaaaaa ttctactaat caaagacatc ttacaggtga tggaattgca
    17041 atagctttaa gaaataatat aaaaatagaa aatttagaat atattcaaat acatcctaca
    17101 gccttttatg aagaaaataa tgaaggtagg agaatgctta tatctgaatc attaagagga
    17161 gagggagcta aacttttaaa taaaaataaa gaaagatttg tggatgaact tcttcctaga
    17221 gatgtagttt caaaagctat ttttgagcaa atggaaaaag ataaattacc ttatgtgtat
    17281 ttagatgcaa cacatttaga tagtgaatat ttaattaata gattttcttt tatttataat
    17341 gaatgtttag ctagaggaac agacataacg aaagaatgta taaaggtatc tcctgcacaa
    17401 cattatttta tgggcgggat acatgtagat ttggattcaa aaacatctat ggaaaattta
    17461 tatgcagttg gtgaaattag ttgtacaggt gttcatggag ccaatagatt ggcaagtaat
    17521 tcattacttg aagggcttgt attttcaaaa agagcagcta aaaatataaa ctcagttata
    17581 gataatgtaa aagttaagtt tatagatgta ccagatatgg atatagatat agaacaggta
    17641 aaaaaagaaa ataaaatatt agtcataaaa gaaattgaaa gaacaagtga ggattttggc
    17701 gatgaattat ttgattattg atagaatgat acaggatgct ttgatagaag atgttccaag
    17761 tgaagatata acaactaatt ctattgtgga tgaaaattct aaatctactg tagatttaat
    17821 ttgtaaacaa gatggtataa tagctggctt aggtgtattt aagagagttt ttgagatact
    17881 tggtgatgta gatgtaaaat tatataaaaa tgatggtgat agagtaaaga atcgtgaaaa
    17941 gatagctgta ttaacaggta gtactagaaa cttacttgta ggagagcgtg tagcattgaa
    18001 ttatttacag agaatgagtg gtatagctac tattacaagt aggtatgttg aaaagcttga
    18061 aggtacaaat acaaaattat tagatagtag aaaaacgata cctaatctta gaatattaga
    18121 taagtattct gttaaagtag gtggaggatg caatcataga tttaatttat ctgatggaat
    18181 attgcttaaa gacaaccata ttggggctgc tggtagtgtt aagaaggcag ttgaattagc
    18241 tagaaaaaat acttcttttg ttagaaagat agaagtggaa gtggaaactt tagacatggt
    18301 aaaagaagca atagaagcta atgcagatat aataatgttg gataatatga gtttggaaat
    18361 ggcaaaacaa gcagtagata ttataaatgg aagagccatt gtagaatttt ccggaaatgt
    18421 aaatttagat actatagaag atataggaaa aattggtgtg gatgttgttt ctgtaggagc
    18481 gcttacacat tctgttaaag ctctcgatat aagtatgaag aatttgagaa atatataaat
    18541 tttatattga aataaactta attaaaatag actccttaaa tttgttttat aaatttaagg
    18601 agtctatttt ttattcatta aaattaataa aaatagaaaa aaatcaatca ataatttaat
    18661 aaaaatagat aaatactata tacactttta ataatatagt taatatctat tttacagata
    18721 aataagaata aacttataat ataattgtat ctgtaaaatt ttgtaaaata taaaaagtat
    18781 agggggaata aaaatgaaag tagcttatat ttttagtagt actaatacac ataaaattct
    18841 agacaaaatg ataataccac aacttgaaca aggaactcat ggagtagacg ttctagggat
    18901 gtttttcttt atggataata ccttattttt atctaaaggt aatcctgtag gcgagagact
    18961 aagtaaatta catgaaaaaa ctgatatgat aataatggct tgtgaccaat gtgctataga
    19021 aagaggtata gagaataatt tggttgatgg agctacaatt ggatgtttcc caaacctata
    19081 tgcttgttta ggcagtgcag gtgtggacca agttataacg ttataaatta gtataggata
    19141 caatccatag aaaaatttat tttctgtgga ttattttaag taaaagataa attgtttaaa
    19201 gtaggggatt aacaaatgtc taaaatttta gttgaattta taaacacttg accatcttgt
    19261 gatgaacatg gagtgtttat aaaaaaaatg tcagaaaaat atgctgataa attggaaata
    19321 aaattatatc aagcagggaa agatttttcg tatataaaaa aatatgggat tataacaaaa
    19381 ggaactttaa taataaatca gaaaaagaaa tatgatagat taaataaaga tacaattgaa
    19441 agggcaatag tagaagctat aaacaataat taaaagttta aaaattagga gtatcaaaaa
    19501 tgaatatatt agaaattgtt ttaaatggag ttaaagatac tatagtagct tctatagtta
    19561 cactgaatgg agcatcagga tggcttatga taagttttat attggcaggt gtacttcaca
    19621 atgttttaac tccaagaagg tttcaaaaac aacttggaaa taagaaattt tcttctttaa
    19681 ttaaaagtac tatctcagga atgtttttac caatatgtag ttgtggagta ataccacttg
    19741 gaataagtat gtattattca ggagcatatt taggaccagt acttgctttt atgacatcaa
    19801 ctccaataat aaatccaata gctgtagttc tttgtctagg tcttttaggt cctaaagtta
    19861 caatcattta tctaatagct ggttttttag taccattttt agtaggattt ttaggaaatt
    19921 gttttggaaa agaagaatta tcacttcata atgattcaga ggaagaagta atagaattag
    19981 aagaagaaaa aagaagtttt atgggaaata tgattgatgg tcttaaatgg tcttttggtg
    20041 aattagccct tactattagt aaatatgtag taatgggtat ggtttttgca ggatttataa
    20101 caactatttt tccaaatagt ataattcaaa aatatttagg aaatccaggt ttgctatctc
    20161 ttggaagtat agcaatactt gcatgtgtta tgtatgtatg tgctgtagga catataccgt
    20221 ttatagcagc ccttgttgca agtggagcat ctcctggtat agcgataaca tttctaatag
    20281 ctggagcagc tacaaatcta ccagaactta taagtatgtt taaacttatt ggaaaaagaa
    20341 ctgttattat atattctgta actttgacac tgtcttcaat aataattgga tatataacaa
    20401 atttactttt agtgaataat aactcagtaa atttagatgc aacaaattct tcaatacagt
    20461 tggcaaataa actaatgtta aatatacctg atagttttga gtatgtatgt actattttaa
    20521 ttattttatt tgcatttaaa tcaataatac caaagatgaa agaggtcata ggaaatcatg
    20581 cttaaaaaga tagttataat atcctgtata gtagttgtgt taatcatttt atcaaaaata
    20641 gttgatgata acatcaaaga agatgcttct attccaaatg tgaataaaga aacattagaa
    20701 tattttagaa aaaattataa agaagacata ataacttgtg ctgaagaaga cttaaataat
    20761 gatggaaaaa aagatttagt agttatatat aaaaaaagca ataattcaaa tgaaatggta
    20821 gtcgtagttt ctgataaaaa tagccactat ataacaaagc caataccagc acctatagag
    20881 aatcaaacga taacctttaa aaatattgat gataaagcac caatagaagt aattgtatca
    20941 gggtctaaaa atggtaatgt aggatatgct atttacagag tagagggtaa aaaatttgtt
    21001 gatttatttg gcgaagatat ggacaaatgt tgctaaaaat aatacataaa ggtgggaaat
    21061 ttatatgaaa ttatctaaga agattcaagc attaatctta tttggtgtaa tgggtacatc
    21121 tatattaaca ggatgtagta gtaaaccaaa agaaaaagaa gaagcaaaaa ctggaaaaga
    21181 tgattataca ataacagtag gttattataa ttgtgaccat atgactgcgg gaccagtagc
    21241 agaatcggca ggaatatata aagatttagg tcttaatgta aaaactgttg gaaatggtaa
    21301 agttcctgaa gctatggcag caggaaaaat ggatgcaggt tacataggaa ctaaggggtt
    21361 agttggagca atacctaagg gttcgccaat aacaatagca gctaacaacc atactggtgg
    21421 ttctgaatat ttaatagttt caaaagatat aaaagaacct aaagatttaa taggtaaaaa
    21481 aattgcaaca gatatgagtg atttcttatg gacaagtgat tatggtccag aaacaggtct
    21541 accaactgac ccatcaaaat atgaagttgt aaatatggat tcagacaaag ataaatattt
    21601 agctcttaaa actgggaaaa ttcaagcttt tacatcttgt gacccttggg gttctgtagc
    21661 tgaaaatgac ggtgcaggaa aaataatagc atctactcaa tataaagaaa aagctaatgg
    21721 caaagaatat aactgttgtt cattctcatt aaacaaaaac tttataaaag aacatccaga
    21781 tttagcaaaa aaattggttc tagcacatac aaaagcaata gaatatatat atacaaatcc
    21841 agctgaagca gctaaaatat ttgcaaaata ttataatgta gaagaagaag ttgcacttag
    21901 aactatctat aagaagactg ttggagaagg tagaactctt acttggaaag ttacaggtga
    21961 agaatataaa aataatttac aaatgtataa agatttaaag gctttagatg atatacctaa
    22021 atatgaagat acgattgata caagcttatt ggactcttgt ggagctgatg attttgacaa
    22081 atttataaaa gaaaaagtta acccagagtt tcctgaaggt atgagttatg aagactggaa
    22141 agcaaaggta ttataaataa atggagaata tgaaaaatat aataatactt tttatcatta
    22201 ttttgggaag tatatttata atatttttta attttgaaga taaagaagaa ataaaaggaa
    22261 aattaagaat aggggtatct gatgatacct ctggttttgt aataaattat atggtaaata
    22321 gagactactt tgaaaatata aaagtaagtg atataatgga tatttttaca attaatgatt
    22381 gctgagcaag tacaatgcag tgggctctga gttcagagga aatagatata gcaatcatat
    22441 gtaaagatgc tgcaaaacaa tatgtaaatg taaatagtgg atttgaaatt gttggtactg
    22501 tagttcaaaa ttctgatata tttctattaa gtgataataa tcctgaaaag ataggagtca
    22561 ttcaaaatag agattaccag tcagaattgg ttaaaaaata ctacaagaat gtggaagtag
    22621 ctcccttatt agggactgca ctagcatatg gattagaaag taatttagta gatggtgttg
    22681 taatagattg cataaagtca attgggttag agggtaaaag aaaatctaca actaaattag
    22741 gagattatga tacctatgta ttagttgtaa ataaagagtt taaaaaaagt aaagcatata
    22801 gtgattttat aacactttac aataaaagcg ttgatgcttt aaagaataaa gctacattta
    22861 agaaagtatt aaaagagtac aggaatataa gaaatataga tatggaggaa ataagcaatt
    22921 ggaaattaaa atttctaaag ataaaagcga tagattaact aagaaaaaaa ctgatggtag
    22981 tatggatatt ttatataaaa taatactatt agttattata tttttagtct ggcattttgc
    23041 agcaaaagat atagggagtt ctttgttact tccaatgcca gtagatgtaa taaaaggttt
    23101 ctttttctgt gtgacagatg cagagacagt cactaatctt tttataacat tgcagagagt
    23161 acttaaaggt tttatgtatg cattattgtt tgggcttcca ataggattta ttatgggatt
    23221 ttctaaaact tttgaaaggg ttttatctcc agtagtagat tctgtaaggc aagttccaat
    23281 aatggcatgg gttcctctta ctatagtatg gtttggtata ggagatggtc caacaatatt
    23341 tttaatagct ttttcagggg ttttccctat aattttaaat actattcaag gagtaagagc
    23401 aatatcaaag gattattata atgcagctag aagtatggga gcatcgccaa ttgtgatatt
    23461 tacaaatgta attgttcccg catctcttcc agatatacta actggttcaa ggattgctat
    23521 aagtacagga tggatgtctg ttatctgagc cgagtttata gctacgagtg ccggtctcgg
    23581 ttactcaatg gtacaagctc aaacattaat gcatacagat gtattaattg ggcttatgat
    23641 atttgcagct ttaataggat ttataataga tagggtatta aagtttataa ataaaatcct
    23701 ttgtagatgg aggtttgcag attaatgaag ttaagtgtaa gagatataaa taagacattt
    23761 gtaaataata gagtacatac aaaggtttta gaagatataa gtatggatat agatgatgga
    23821 caatttgtct gtcttttagg accatcaggt tgtggaaaaa caactttact tactataata
    23881 ggtgggtttc aaaaatcaga acgtggagat gtttttataa atgataaaag agttaaaaaa
    23941 ccgggcatag atagagcttt tatatttcaa aattatgctt tatttcattg gaaaactata
    24001 agaggtaatg ttctatttcc tatgaaacag cagaaaatac caaaagaaaa aagagaagaa
    24061 atgctagaag aacttttggt tatgtcagac ttgaaaggaa aagaaaaact gtttccgcat
    24121 caattatctg gaggaatgaa acaaagagtg gccatgataa gagctttagc atgtaatcca
    24181 gaggtattac taatggatga accattagga gctgtagatt ttcaaatgag acaaaattta
    24241 caggaagagc tagaacgaat atggataaag aagaaaataa ctgcattaat ggtaactcat
    24301 gatgttgatg aagctgtata tatgagtgat agagttatag taatgtcgag agataaagga
    24361 agaattattg atgatataaa tatagatatt ccaagaccta gaattagagg aagccaaaaa
    24421 tatgaagagt acaaaaacaa attaactgat actttatcaa agtgttatga agtttaaaga
    24481 ggagtagaca tgagtcaaat agaaaaatac aatagtttgg ataatacaca acagaaaata
    24541 agaatctcta ttatggatat gataatagat aaaggttcta gtgttacact tcaagaagtg
    24601 actgaatatg taagtaaaaa gttgaatata gaaaaagaat atatagaaag gacactacaa
    24661 tattttattt ataaaaatat aatggttgtt gatgggaata gtataaattt tatatatcct
    24721 gtatcagcat tgccaacaaa tcataaagta actttgagag ataatagaag tttcagtgca
    24781 atgtgtgcta tagatgctat aggaacctct tgcacattca accaagatat aaaaataaac
    24841 tctatttgta gtgctacagg taaggaaata gaagtaatta taaaaaatga aaagattgag
    24901 tatgtgaata atccaaactt aagagtgctt catataaatc ttgataagca tctaaattgg
    24961 gctgctagtt gctgaaatat aatgaatttc ttctggacga agagtgattt tgatgcttgg
    25021 acaaacgaag cgggattatc tgatgatgaa gatatatatt gtttagatat taatgaggca
    25081 atagtagaat catataaaat atttaaactc aaacaaaagg tactatcata gatatacata
    25141 tatgaaaaac taaaaatgac ctttagataa aaatgatagg taatatccat actatataaa
    25201 ttagtataga gtagggatgt gaaaagtatt taaatctaaa ggtatatttt tagttttttt
    25261 tagatgatta gatagcctta ttaagtatat atttatttat aacctttgca actccatttt
    25321 cattattagt atcagtaaca taatctgcaa tttcctttat actattacgt gcattcccca
    25381 tagccacacc aagacctgca tactttatca tatgttggtc attttcttca tcgccaacag
    25441 caatagcatt ttctataggt atatttagat tcatacataa tgttttaaga gcagttcctt
    25501 tattagtagt tttacctaaa aattctagat aaaaaggagc agaccttact atagtatatt
    25561 tattgaataa atttttaggt agttgctgta tgatttcttc caatctttct ggttcatcaa
    25621 ctatcataat tttacagatt ttaatagatt catcaatttt atctttttgt atatatttga
    25681 cttctatatt atttaatttc gcttcatgaa gtgtatattt gccaggtgtt tcatttggtg
    25741 taattaaatt tgtcattgta tttatgtgta ttttagtctt atatttttta caaacgttat
    25801 tatatataaa atcaaaatca tctaaagtca tttctacatc atgtatgatt ttctcacttt
    25861 taacttcttg aataattgct ccattaaagc atataacata atcaccttca tctgttagat
    25921 ttagttgatt tagaagttct tttacaccag gaagaggtct gcctgttgtt ataactactt
    25981 ttgccccagc cctttttgcc tcctgaatgg actcaaagac ttcaggagta atcctttttt
    26041 gtgtatttaa tattgttcca tcaatgtcta gtgcgattaa tttgtacata tcaacctcca
    26101 ataaattata tactcaaatt tcctgtgaaa acgttgacac agctagtgtt gaacaaagtt
    26161 tcagtgggaa atatggtttg ttttgtatac tattatatca agagataagt aatttagaaa
    26221 gtttaataat attaattttt tgataaaaat attacttatg tgagatgaga atgtaaaaac
    26281 tttgtaatca ataattccaa aattcttaat tacaaataat ggtatcgttt gcattgttaa
    26341 tataaataaa tgtatattat atagttagaa gtttgaataa atagactgat ggtaatttgt
    26401 tatattagtt agttttgtgt tataaaatat tgtaattcca ttaatcatat gttattattc
    26461 aacatgatta taatataaag aatatttgat atatataata atttataaaa gagtattaca
    26521 tagtacatgt aaatctatat ttataaatac aataaagtaa ggttgtaaag gagtgcttta
    26581 aatgaagttt tcagagttta agtatgaaag acccaattat aatagtatga aaaaagagtt
    26641 tttgagttgt gtagaagata taaataattc tcgtaattat aaagagcaac aaaaaaatat
    26701 acataagatt aatttattaa gaaataaaat agaaacgttg tcaaatatag cttcgataag
    26761 atatagtaca gacacattta ataaattcta caaagaagaa aaaaattatt gggatgaata
    26821 tatgcctttg tatgaagaat taaattcata tttttataat gccattgtaa attcaaaatt
    26881 taaatatgat ttgataaaag agtttggaga gcaatttttc acaatagtag agtattcttt
    26941 aaaaagtttt tccaaagaaa taataagtga attgcaggaa gaaaataaat tgtgttcaga
    27001 gtatactaga cttttagctt cagctgagat aatgtttgat gggaaaataa gaaatttatc
    27061 tggtatgggg aaattcatgt attctaagaa tagaaaaaca agagaattag caaacaaagc
    27121 atattataat ttctttgaag aaaatgaaac taaatttgat gatatatttg ataaattggt
    27181 taaacttaga gataagatgt ctaaaaaatt aggttttgaa gattttgtag agcttgggta
    27241 tgttagaatg atgagaagtg actacagaga atatatgata aaaaatgtta gaaaacaagt
    27301 actaaaatat gttgttccaa tggcaaatga gctttatgaa aaacaagcta aaagaattgg
    27361 tctagaatat ttaagttaca tagatgaagg cgtggaattt ttaacaggaa atgcaagttt
    27421 aaagggagat tcaagataca taattcaaaa tggtaaaagg atgtattctg aactgtcaaa
    27481 agaatcaaat gaattttttg attttatgtt agaaaatgaa cttatggatt tagaaacgaa
    27541 gaaaggaaaa ggagctggag ggtattgtac ttatatacca gattataaat caccattcat
    27601 tttttctaat tttaatcaaa ctgctgatga tatagatgtg ttaacacatg aggcaggaca
    27661 tgcatttcag ttgtatatgt ctagatggat tgatatgcca gaaattaatt ttccaacgct
    27721 tgatagttgt gaaatccatt caatgagtat ggaatttata acatggccgt ggatggattt
    27781 attttttaaa gaagatacag ataagtacaa attcactcac ttatcatctt caattaaatt
    27841 tataccttat ggagtaattg ttgatgaatt tcaacactat atttacaaaa atcctaatgt
    27901 agataagtca aaacgaaaag aaatctggag atttttagag aaaaaatact tacctcatag
    27961 aaaatatggt gataattctt ttttagagag aggttgttgg tggtttaaac aaggacatat
    28021 atttaaaaat ccgttttatt atatagatta tgtattagct caaatttgtg cgttacaatt
    28081 ttggaaaaaa atgattcaag atagagatgc aggatggaaa gattatataa atatctgtaa
    28141 agttggagga acaaaatcat ttttagatat tgtgagtatg ggaaatttgt attctccatt
    28201 tgatgatggc tgtattgagt ctattatagg tgatgttaaa acttggtttg atgaaataaa
    28261 tgacagcaaa ttataaaaaa ttatgagaat tttattgtat aaaagtgaaa aattaaaaaa
    28321 tatataaaaa gtgattattt gttgtgaaaa tagtcttgtt tttaactaaa ttgataaaat
    28381 aaactaaaaa agatttgtac ttaaaaaaaa aagaaaatac aagaaatatg caatatcaac
    28441 atttggaaaa aaaagtaaaa atgtatacaa aatacatata aaaaaactat tgcaaatttt
    28501 atagaatgtt tatatactta agtcaacaga gatagaaaaa agctatttat agaaaacaaa
    28561 ggataaattg cttaatttta aattttctga aaaaaataat aatccggatg aagagtatgg
    28621 gagactgctt tataaaaagc gaccgaaggg acaagtttaa tcttacttac ccaaaagatt
    28681 agatgaaatt ctcaggtaaa ggtgactgta cttggacggg gctctgaaaa tgattttaac
    28741 agggaagaca aattgggaga tttgtttttc ctgtttttta tttatcaagt aataaataaa
    28801 agtttgaaag agagtaaaaa aatgataata attacgaata atccaaaagt aaaagaagaa
    28861 gttcaaggca gagaagtttt gtttaaagac acaacttata ttggaatctt agaagcaagt
    28921 agagatttga tacatgaagg ttatgaactt ttatctcatc cactctatgg gagtgtaaag
    28981 ccaaatgaaa caccttacag gactgttata ctaaagaaag gaaatcgttt ggatataaac
    29041 tctttaacct taattgaaga agcaatcatc actgcaagta aatttcagaa taataaaaag
    29101 actccaaaat ggacagaaag tgttcaagat gattttagag taattgatta tgatatattt
    29161 tataatacaa tacaaagaat gcagtatgaa taaaaaaatt tgttaaaaag ttattaaaac
    29221 agcctaaata ttaaatctaa tttaatatta aataaaatga gaaatttaat tggggaggat
    29281 aagtcaaaat ggaaaatgtg tatgacttag taataattgg ttcaggacca gcagggcttg
    29341 cagcaggtct ttatggagca agagcaaagt taaaaactct aatcctagaa aaagacaaga
    29401 ctggtggaca aattgtaata acacatgaaa ttgcaaatta tccagggtca gttccaaatg
    29461 caacaggacc aagtttaata gctagaatgg ttgaacagtg taaagagttt ggagcagaaa
    29521 tgctaagaga caacattgtt gatactgagc tagatggaga tataaaagtt ttaaaagggg
    29581 aaaaggcaga atatagagca aaagcagtaa taataggaac aggagcaaca ccaagaaaga
    29641 ttggttgtcc tggagaaaaa gaacttactg gtaaaggggt ttcatattgt gcaacttgtg
    29701 atgcagattt ctttgaagat tttgaagtat ttgttgtagg tggaggagac agtgcattag
    29761 aagaagcaat gtatttaact aaatttgcta gaaaagtaac aattgtacat agaagacaag
    29821 gatttagatg tgctaagagt gttgaagaaa aagcaaaagc taatccaaag atagagtttt
    29881 tattagatac agtaattgaa gaaatcaaag gtgatggaat attagaatca gttgtattta
    29941 aaaataaagt tactggagaa actcatgaat actttgcaga tgaagaagat ggaacaatgg
    30001 gagtatttgt gttcgttgga ttagatgctc aaactgattt attcaaaggt aaagttcata
    30061 tggatgaaaa aggttacata ataactgatg aagatatgag aactaacata ccaggcgtat
    30121 ttgcagcagg agattgtaga tctaagactc taagacaggt tgtaactgca actaatgatg
    30181 gagcaattgc atctatagta gctgagaaat acattgatga aaaatttggg aactagaatc
    30241 tgtaaattaa gaaatagtgt ctgtaaattt aatgaataat atttaaaatt aaaataaaaa
    30301 tttcaagggg gaataagaaa atgttagatt tagataaggc tacatttgaa gaagaagttt
    30361 taaatgcaga aggttttgta tttgttgatt tttggagtga aggttgtgag ccttgtaaag
    30421 ctttaatgcc agatgttcat aaattagctg agacttatgg ggataaaata aaattctgta
    30481 agatggatac aacaaaagct agaagattag ctataaaaca aaaagtatta ggtcttccaa
    30541 ctatggctat atacaaagat ggagaaaaag tagacgaagt tactaaggat gatgctacag
    30601 ttcctaatat agaaaacatg attaaaaaat atctttaata tgttgataaa ttattaacat
    30661 taaaagaaaa aatctatttt tatctgaaga taagaagaaa aaggttttta tgtccaactt
    30721 tgttaaaaaa ataattttaa ataaaataat ttttttacaa taaatatttt tta
//
LOCUS       DBGJFF010000034        30707 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.034, whole genome shotgun sequence.
ACCESSION   DBGJFF010000034 DBGJFF010000000
VERSION     DBGJFF010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30707
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.034"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            138..1136
                     /locus_tag="ACOIAF_001619"
     CDS             138..1136
                     /locus_tag="ACOIAF_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HGZ2668633.1"
                     /translation="MKILVFGARDYEEPVIKKWSEEHKDVQVDIYPENMTEENVVKAK
                     GYDGISIQQTNYIDNPYIYETLKDAGVKVIASRTAGVDMIHFDLVNENGLIVTNVPSY
                     SPNAIAELAVTQAMNLLRKTPLVKKKVCEGDYRWIAELLGTEVRSITVGVIGTGKIGA
                     TSAKLFKGLGANVIAFDQYPNSDLNDILTYKDSLEDLLKEADLITLHTPLLEGTKHMI
                     NKDTLAIMKDGAYIVNTGRGGLINTGDLIEALESGKIRAAALDTFETEGLFLNKKMNP
                     GELTDPEINKLLSMEQVIFTHHLGFFTSTAIENIVYSSLSSAVEVIKTGTATNRVN"
     gene            1498..2025
                     /locus_tag="ACOIAF_001620"
     CDS             1498..2025
                     /locus_tag="ACOIAF_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04002 family protein"
                     /protein_id="HGZ2668634.1"
                     /translation="MEKMNKVTSKKTTYIVTSALFASIICLTIAYILHIPVGGNNGYV
                     HIGDAFIYLAATILPTNYAIAASAIGAGLADLSTGAAIWVIPTIIIKPILVLFFTSKS
                     DKIINKRNIVASVVAGIVGLVLYMFAEGIIIGSFTSAFVMSLLGLLQPIGSFIVFIIL
                     GMALDKLDFKKRYFN"
     gene            2052..2648
                     /locus_tag="ACOIAF_001621"
     CDS             2052..2648
                     /locus_tag="ACOIAF_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04100 family radical SAM protein"
                     /protein_id="HGZ2668635.1"
                     /translation="MNILYTIENSIYVNITNTCPCSCVFCIRNEKDEVANSGSLWLEH
                     EPSVDEVKEAFNKYNLDDYDEIVFCGYGEPLMRINELIEVAKFIKEKSSIKIRINTNG
                     LSDLIHNKKTAILLKNVIDAVSISLNAPNKEAYNRVTQPKFGEKSFDYMLDFAKDCKK
                     YIKEVAFSVVDEISPEEIEESKQLAKKLDIPLRVRHKN"
     gene            complement(2727..4310)
                     /gene="asnB"
                     /locus_tag="ACOIAF_001622"
     CDS             complement(2727..4310)
                     /gene="asnB"
                     /locus_tag="ACOIAF_001622"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HGZ2668636.1"
                     /translation="MINLEEKYLTRKDTYMIKILEYNGHIRNWKVLCEELGIDSSLCR
                     DERERAILVEAYKTWGYDMANHMHGMFAFALWDTVEKKLFCLRDQFGVKPFYYYETEN
                     GKLLYGSSIRKIMEQPGFVKELNEDMLQIYMSFTYVAGENTFFRGVKKLMPGHYLVYQ
                     NKTVCIGRYWKPEFHPDHSKSIEEWTDEIHTTLQKIMPEVKSENETAELFLSGGVDSS
                     YILAMSDIEKTGSCGYEEERFDESKLAQQTANILGCKNSRYIITPEEYFASVPYVMYH
                     MEQPTGDASAIVFAIGCKAAAENTNICYSGEGADEFFGGYNIYHKAECYGENLKTFYI
                     GNTNIMNEGEKRKILKKYNPDVLPIEVVKGIYEETEELDTLTNMSNVDIQIWLEGDIY
                     FNIDKMSTAVGLEVRMPLTDTRIFDIASRIPSEYKVKGKENKIAFRTAASKTLPEEIA
                     FRNKLGFIVPIRIWMADDQYNKDIRDKFNSEIADKFFNIDEINEIFNDYISGNSDNWR
                     KIWMIYTFLVWYEIYFVKC"
     gene            complement(4912..5748)
                     /locus_tag="ACOIAF_001623"
     CDS             complement(4912..5748)
                     /locus_tag="ACOIAF_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HGZ2668637.1"
                     /translation="MNYIIKKVLNSSVVLVHDTKDNEFILLGKGIGYGKKTGEYICGS
                     NDNQMFVPVENTKSKQFLELMDDIPVDILKITQEIIVEAEKLLNSSFNKNLYLILADH
                     FNFAIERMRKGIKITNRVFWEIKNYYPNEFKVGMVAIYMVEERLGIQLPEEEAANIAF
                     HFANAMASDGNSYDTIKYAKVIGEIINIFVFSLNRTLDKKSMHYMRFITHIKFFVERF
                     FSDSMLSSGDDLLFNQMKRSNPKEMLIALKVRDFLEKKYGKKLTNEEIAFLVVHIARV
                     KQ"
     gene            complement(5772..7238)
                     /locus_tag="ACOIAF_001624"
     CDS             complement(5772..7238)
                     /locus_tag="ACOIAF_001624"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HGZ2668638.1"
                     /translation="MRKFPEGFLWGGATAANQFEGGWNLGGKGWSVSDVAKAHFDADV
                     KDYKSNNEITTKDIEEGLAHPEDEVNYPKRHGSDFYHHYKEDIALMAEMGFKTYRMSI
                     AWSRIFPNGDDKEPNEEGLQFYDDVFDELISYGIEPLVTMSHYEPPLNIVLNYDGWYS
                     RQVINMFVRYVETICERYKNKVKYWLTFNEVDSMIRHPYTTGGLVRDRFKDKNFEEVI
                     FQAMHHQFVASALATKICHEIIPNSKVGCMLTKLTYYPYTCRPEDVLATQQKMRSIYA
                     YSDTQVFGEYPVYLLSYFKNNNIQIVKEEHDDEIMKKYPVDFISFSYYMSSCEAADTT
                     GLDITPGNTLLAVKNPYLEMSEWGWQIDSIGLRISLIELYDRYRKPLFIVENGLGAKD
                     ILTEDKKVHDQYRINYLKEHFKCMLDAIIEDGVELWGYTSWGCIDLVSESTKQMSKRY
                     GYIYVDADDYGKGTYNRYKKDSFYWYKKVIENNSIDFK"
     gene            complement(7241..9103)
                     /locus_tag="ACOIAF_001625"
     CDS             complement(7241..9103)
                     /locus_tag="ACOIAF_001625"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HGZ2668639.1"
                     /translation="MNYKKTAIDIIEQVGGSKNILALEHCSTRLRFKLIDRSKVNQDA
                     LKKVQGVMSIVNGAQFQVVIGNSVVDVYDEILKVCPLDTNDSTVKTEEKINLGTRFIE
                     FIINIFQPLIFAIAGAGILKSLLMLLAMFNILKSDSTIYTLLVAISDATFYFLPLMVA
                     VTTANVLKCNRLVAIAAVGYLLLPATTTALSEGVELFGFTIPNIAYNAQVFPAILCVS
                     FLATMEKIFNKYSPKPIRTFFVPMMSLAITVPITLTILGPLGYNVGTIFTTIILFLYN
                     KMGFLVVGLLAAFLPFMVATGMHKALIPYSINTIGKLGYEALYMTSSLAHNISESGAC
                     FAVALRTKDETLKQTALSAGISALMGITEPALYGITLQHKRAMLGVVFSSAISGTFLG
                     LFAVKGFVIVGPGLASMSMFIDPNNGNNLIFASIGFVIAILGSFIITLIIWKEDTNTV
                     ESSIGDVVEEELEEEKEVNILVTPVEGKVIDLSKVNDELFASKTLGDGVAIIPTNGNL
                     YAPCDSEVVMLFETKHAIGLRTKNGAEILIHIGINTVSMNGDGFKTFVKTGDNVKEGD
                     LLIQFDLDKISHANLDSTVMIVNNNGADYAYKVLNQSYGNVKKGSILFDVKGGI"
     gene            complement(9350..10213)
                     /locus_tag="ACOIAF_001626"
     CDS             complement(9350..10213)
                     /locus_tag="ACOIAF_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HGZ2668640.1"
                     /translation="MILSYFALLIRVVLLAFERVVVRLLGDEEGDIYKNIASSFLFFF
                     IGGVCLLPFSIMDRVSDWSFLIPCYISSLVYSIGSVAYVTSLATGEVSLVTPINSLNS
                     LFLLLLSVIFLGEGLSLAKVAGIIIMIGGVFILKKVSSFLKNVSVIFNNLPCRLMFLY
                     IFLQSVGRVLDKAFFVQVSPILYSTILYFFVGLNLFIFLAFKKKQKIICEIFTNKKGL
                     SILSGAINGYSYLALLIALNNLELSIAEPFSQVSMIITLILAHFIFKENIKEKIPGSI
                     LILIGGWLLLL"
     gene            complement(10267..11199)
                     /locus_tag="ACOIAF_001627"
     CDS             complement(10267..11199)
                     /locus_tag="ACOIAF_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HGZ2668641.1"
                     /translation="MNNKKAVFFFCSDLKKDPVASNVIKCCEEIMDLNQTDIVIDDNF
                     VLEFKDKNNNLFHFVKTKDVISHNYKYYLPILNRHFRDYDFAGVVNWHEGENAPEHIL
                     TAHTIGDVPTGEFGNSNPRYFKNLINAIEDIKNENLLDEFTTLTEATHWSGTTYGEES
                     KLITEYSVPMLDIEIGSSSDSFNNSIAIQVLAKSLIRVFDCDEPLKTLLCVGGVHFEK
                     SFSDIIKNKEYNISIGHVLPNQWIVSGMYDDESGFEKLEKCINSIEGGIDCIVFHDKL
                     KGTYKEQCRKLGEKLNVPVFKHKILKNPKDLPIW"
     gene            complement(11285..11836)
                     /locus_tag="ACOIAF_001628"
     CDS             complement(11285..11836)
                     /locus_tag="ACOIAF_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGZ2668642.1"
                     /translation="MQSLNEIIAENLKKIRKEKHLSLDKIAQLSGVSKSMLSQIEKCE
                     VNPTISTLKKITNGLKISFTSLMERQESDIELIQKSDIDHFIEDNGKYISYPIFPFDS
                     KRRFEIFMIEIEEGGNLDSNAELPGTQEFITVFSGEVTIKINGEDYIVSSGNSIRFKA
                     DVSHIYRNSGKGIAKMSMVVYYI"
     gene            complement(12065..12556)
                     /locus_tag="ACOIAF_001629"
     CDS             complement(12065..12556)
                     /locus_tag="ACOIAF_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668643.1"
                     /translation="MIDLKIENRKGKIHVKSSEIKDILELRPDFEYVQDISNTINQEN
                     ILVFDCQLTGNSFDMDDLDIEEILEELGEEIDESYFSVLFEDVRAYLKDATDEIEADL
                     QDNYLFDNLRCYFDIYNINQEFTDFKFVFLVSFKDIKISSLANLAKIVSKRQLIGASK
                     FYS"
     gene            complement(12584..13144)
                     /locus_tag="ACOIAF_001630"
     CDS             complement(12584..13144)
                     /locus_tag="ACOIAF_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HGZ2668644.1"
                     /translation="MNKLNICIDIDGTITSPYHFLPYLNDIFNKNITEAECITHNWEE
                     LYGSGIRDVYAEFNNKYIHSYDEAKIVEGATEVISTLSKGHNLSFVTARHECLTDVTK
                     NWLSRQGFSDIDVYLLGSDYKVEKARELSCDIFIEDNPLNSVQLADDGVKVILLDTNY
                     NKDVKHQNIVRVSNWIDIEKIIKQGI"
     gene            complement(13296..14453)
                     /locus_tag="ACOIAF_001631"
     CDS             complement(13296..14453)
                     /locus_tag="ACOIAF_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HGZ2668645.1"
                     /translation="MNKQSIGKLNMFFMGITQRNLDNRDFFIELEVIFKSGRKEFKGI
                     AIEEDNKYKFNFKGKSDLYTFNELLKNISKEAENYDGLVFKYVERGTIVVIEGDNKKV
                     NVKYLDNKEEVPKIDEFTASQIKNRDYYVKVGQANALLKEIGVLTKDGKIKNDKIRKY
                     NQIDHFVELIDSILKEIKDKDCITILDCACGKSYLSFVLNFYIKEVLKKNCYFIGIDY
                     SDVVIEASKNMAKNLGYKNMSFIKEDLTNYTPNRDVDLVISLHACDTATDMAIGLGIR
                     AKSEAIVVVPCCHKELLGQYRYEAMEPILKHGVFKARFADLITDGLRTLLLEGNGYDT
                     SVVEYISPLDTPKNLMIRAIKTKTNNDKALKEYKELKSQFGVEPTLEKLIY"
     gene            complement(14490..15431)
                     /locus_tag="ACOIAF_001632"
     CDS             complement(14490..15431)
                     /locus_tag="ACOIAF_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668646.1"
                     /translation="MRWILVFIFLYLIPLVVIFRNYKNFKRSCIYSSIYVVLATTIMI
                     SNIYMSGLNKIKESMYYHNYISDETYDEKYVSNFKEKESTDLKQDNKKNEEISNNKNL
                     NNKQDRNIQNKESNEEGSNDIKEKNNSDSNVSVNNKSEKQEDNTKENNKYTSKEKNSE
                     DIKKIDMESIGIFKKEIYNIERAALVPMRECIPYTKDIAKNITKLSSIKKDVTNARNK
                     CKEVVKEYENMQIPELSKEKYTETLSNARDDVKKAYELREKSMESALKLINTKSPKYI
                     GKITEYLDLSDKQIESFKNRLNDLKEEINNSETNNIN"
     gene            complement(15518..16210)
                     /locus_tag="ACOIAF_001633"
     CDS             complement(15518..16210)
                     /locus_tag="ACOIAF_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HGZ2668647.1"
                     /translation="MSLYAIGDLHFSTSVNKPMNIFGSNWDGHEKKIIDNWKEVVEEE
                     DMVLVLGDTSWGINLSEAKKDLDIISKLPGQKILIKGNHDYWWTTVTSLNKLYEDMRF
                     IQTNFYEYKDYAICGGRGWICPNDVKFDETDEKVYKREEHRLRLSLESARKSGHSKII
                     VITHYPPTNDKLEESLFTKLFEEYNVEKVIYGHLHGKESFKMGLKGIRNGVEYTLASC
                     DYTEFNLIKVHD"
     gene            complement(16319..17131)
                     /locus_tag="ACOIAF_001634"
     CDS             complement(16319..17131)
                     /locus_tag="ACOIAF_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HGZ2668648.1"
                     /translation="MEYEKWNEILAPYEHAVEELKIKFKNIRKEYLAKGEYSPIEFVT
                     GRTKKISSIISKLKRINAKDIETEIDDIAGIRIMCQFVEDIYAIVDLIKVRNDMTIIG
                     EKDYITNYKDSGYRSYHVIIKYPINSIAGSKEIICEIQIRTLAMNFWATIEHSLKYKY
                     DHYIPETLAERLRRASDAAFLLDQEMSEIREDIMKAQAMYQMKSIAIRDTLNRIQELY
                     DLGDTHKAMQYQRKLDRTETDKNITEILELKQEIDILLEQYKDTKVDDKVVQ"
     gene            complement(17182..18093)
                     /locus_tag="ACOIAF_001635"
     CDS             complement(17182..18093)
                     /locus_tag="ACOIAF_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HGZ2668649.1"
                     /translation="MNLTKVKGNTFFIKGGTNTGIYVFEDNTALMIDPGLCGSRPRRV
                     NKVLDENNIQVRYIINTHGHDDHYGACNQFKEYYKDVCIMSSEYDKLYIENPELFSKY
                     IVGGKSNVFMDNILKNKMLDDIKIDKSIEAGKIELNNEIFDIIEFQGHTPGSIAVLTK
                     DNVIFVGDLLIGDEILSKYDFLFLYDIQEQINSLEKLKNIDFEYLVLGHGKQVISKED
                     SYKLIEKHLNALKKYLYQIRELLVEPKSLEILLKNIINNNNLSNNYKEYHFFKSSLVS
                     VISYLVDLEEIGYILENGELLYYTKTK"
     gene            complement(18152..18883)
                     /locus_tag="ACOIAF_001636"
     CDS             complement(18152..18883)
                     /locus_tag="ACOIAF_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HGZ2668650.1"
                     /translation="MTSIKNKDILNCIDYSIKNKLFDKLNDVYKSLPVGNCSGCGNCC
                     MESVGINLIEFLNIFCYLEDRVDLKKSCISKILDYYFEEYTRKNPCPFKDTNNRCLIY
                     EVRPLNCRMFGHWKKEDYNKNLDNVIEKNNNYRELMKKQYGFEINDEVVNYRIDYCDR
                     FIPNKDYLSKSKRLSFFDELMILDSNIYSSGNIDIDFRDRGIVEYFIESLLYRNLAYN
                     VKIRISKEYKIRKRTIDRIKRIVLV"
     gene            18972..19223
                     /locus_tag="ACOIAF_001637"
     CDS             18972..19223
                     /locus_tag="ACOIAF_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668651.1"
                     /translation="MNRYTKIINMMGSYYTKDFEKEKKNVIKVREVKEDTVRKFFLQG
                     DCEVLVVFEDTGKEILIDDFSPEEDIKKYLGAKFINKKR"
     gene            19456..19875
                     /locus_tag="ACOIAF_001638"
     CDS             19456..19875
                     /locus_tag="ACOIAF_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668652.1"
                     /translation="MQIYFRVKAVGKRKPMLELTPFDVSDNINCLKEAISEIVSKNVL
                     EYNEKIPEKSIINFLTNEEIESQADIGKVGFGSIYNENKQSVEKAIEVALQAFEDGIY
                     KVLINEDVIEKLDSSINLKSGDIFTFIRLTLLAGRMF"
     gene            19900..24957
                     /locus_tag="ACOIAF_001639"
     CDS             19900..24957
                     /locus_tag="ACOIAF_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HGZ2668653.1"
                     /translation="MLERTRVEKNPKIIENFKNNFTKSSSKQYYDLAKSLWDCIENSS
                     YYYREPEDKFLNMIKKYYNCHYYEIKNMDELLSGPLKNALELIFTSEQVEELCQLIKR
                     KMDYTYSGGYYRKGYRSNKLVDYFRGIIRFISNYVDLIYYDFSVIDYLTLPNKVLYYD
                     SIMSDKIALEIDKGNQKVISALEEVIFGENQTALLTRQMIIGIMKSHSDKCYEMLSKL
                     LLAAKQQEGLRQQIVESLDEGTISASIYMLKTILDNNLERFSSVIRALDTWTGLGFSD
                     QKPGIIRKCLELSYKSLTDEKFRIESINSNDNLEIYFALWATATYNIYDSYSLIENLL
                     SCQEKYKKLVALYFLSHTESTYFKHSIASKNIKGDDLEILAWVITNFYVEGSVGYYYS
                     WQNRETNFDLSNTPIDKDFDARKEQFESLKHTLEILNSKTKSFSGSVFPWTWIEISSH
                     EILKCMMTLTAYDMNAQFIDVLMENCHLMSSDLRASLIVNFVNEPKTDRQREFLLECL
                     SDKSVSNRETVLSLLRKLELTDVELDKITNLLSLKTSSIRQTVIKILLEQDDSNLENI
                     ASSLIKSSNSQKCLGGLEIVNLLKDNTARQELYNKLVLLIEDIDKSKKNTSQVQILID
                     NLLGKAKNDYCKENGFGIYNHENKVQIPEQFDFKDDSNSSSKLNKIISNISLSTREDS
                     VSVATKKEAKKILDTDINKIIKVYEKFDELITENADYEYEVERWSGNVDKVLLGYSTY
                     NLTPIKYKVSNDTLDNYPLAELWRETSKKLDLDYKLILDILFYSISTCGYNETEKSWF
                     SSIYSNVLDSKKQNKIYTKLEKLKFNGLTRKLLRVLLNEFDHKEIFNYCLEIYYKLRN
                     LLTIEQCKKQRVEFTKNYYRQNEYCPALNAPILNYWLDKAHSFIYDDISFTKFFYTTF
                     SNYQLLEYKFPFYTSINVFARAHELGLMTEEQVNREMISGYNASNIIRTLTNPRWAGD
                     TLEKYPWLKDNLKTVTNRIIEIESRRGDMPTEVSHLANNIQYFEGINYFVNILVALGK
                     ETFQRGYSYGSNYSKKDVLSSLLKNCYPTKEDDSKKLKSLLKNTDITELRLIESAMYA
                     PQWVNIIEKYLNWKGLKSTIWYFHAHINEGFSAQKESEVALFSPISPRSFIDGAFDVD
                     WFLDSYKTIGEERFNILYKSAKYITSGGNSHRRAQLYADATLGKLDKDMLMKEIETNR
                     NQDKLRSFSLLPMKKGDLKEATFRYEFIQKFAKESKQFGAQRRESESKACTIALENLA
                     RSAGFGDVNRMMWALETEKLNEVAHLFKPKDIDGTSIWIDVDENGKASTKVQKGNKSL
                     KSIPKAIAKHPYVLELKSVQKDLKSQYSRAKQSLEQAMKNEIVFTVKEIANIYKNPVI
                     CPLIKNLVWVCDDNIGYVYNDKSKFFICNIEGETYKLTAKNKLKLAHPVHLFESGKWS
                     EFQHDLFSKQIVQPFKQVFREYYRINSDELSEKTISRRYAGHQIQPKKTVALLRNQLW
                     TVDYEEGLQKVFYKENIVAKMFALADWFSPADIEPPTIETVEFFDRKTYKSLELASIP
                     PILFSEVMRDIDLVVSVAHAGGVDPETSHSTVEMRIAIAKELLLLLKINNVNFEGSHA
                     LIAGNLGEYSVHMGSGIVHKKGMGALNILPIHSQSRGKIFLPFADEDPKTAEIMSKIL
                     LLAEDSKIKDPSILSNL"
     gene            complement(25142..25879)
                     /locus_tag="ACOIAF_001640"
     CDS             complement(25142..25879)
                     /locus_tag="ACOIAF_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HGZ2668654.1"
                     /translation="MIKVLICDDDKIVRDDIANIAKESGYVDYVKKVKDGIEAIEFIK
                     NEKIDLLIIDIDMPYKNGIDSAEEILSIDKDIHIVFVTGFADYSLESFKVHPIDFVVK
                     PFKKEKILDSINIAIDHINSHKIANNNFIEDTLFVYKIRKQIHMINFDDIVMFEKNSR
                     AINLHTKDEDIIKFYENFDDLKKRLPPNFFMTHRQYIVNLRFIHKVIPINKSSLEIRF
                     INFQENAILNKSLEKDFLYRFYRVKRF"
     gene            complement(25898..26833)
                     /locus_tag="ACOIAF_001641"
     CDS             complement(25898..26833)
                     /locus_tag="ACOIAF_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HGZ2668655.1"
                     /translation="MDNNFVFPIVILTLFIFSNLIMNKYKFYKELDILKRGILTFFIS
                     CFALILFMEGTKYIQLRLGYSFLLEGVLNIILFLVLIILSFYTGFKVIRLINNNNEQE
                     YKLQKLDSKINHKDNIIIKLRSQKHDYLKHLQVVYSLLNTGLDEDAKEYIMNISNSFK
                     SKNSRYGKVSYLDAIASLKYEECLEKDISFDVYIQDFMDNLEIEPNDMSSVLLNIIDN
                     AIESLSTIKKEKKYIRLHAYEDEFQYIIAIKNNGPKIHNVESIFWNGFSTKEGQDRGY
                     GLHLVKNILDKYDYSILVNSDDFLTEFVILIPKYY"
     gene            27041..29617
                     /locus_tag="ACOIAF_001642"
     CDS             27041..29617
                     /locus_tag="ACOIAF_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HGZ2668656.1"
                     /translation="MKDNLKIVLRYIKSYKARSLAIVVSIILATSLVVGIGTLSRSAQ
                     QADVDRLKRELGTDHVYFKNIKKNQLKSIKSSKDIKNLGITSYYASTDVNEKLPINIL
                     YANKNYLTSHSKLIKGRFPKASNEVVVEKWILNSMGLKPEINQNLTFKLYQKENPETF
                     KVVGILEDRFIEKKRGQCEIFSNLNESELNNFTTYIEFNEGSDINTNIDNIIKNAMLD
                     EKSIGRNSMLIESTMKNGTLDNSSKYIAIAMSLFAGIVIYSIYVISIYQRVQEYGILR
                     AIGSTNFRIFKLMLYELFILALIAMPIGICIGMGGAQIFNRSVGNIQFEGNINVTPFV
                     IPDKIILLSIACTILIMLIISFFTYLKIRRISPIDAIRKTFGSDKNVTKVNYLISKLT
                     LNMSVTKSISAKNIFRNKKGFMIIVLSMSIGGIIVIKDNYKYSFLDVQNKSVQEQTYM
                     NADFILSNVYLNETGNNTSFKDIKGFSDSQIDKIKNIDGIDKVKTASILNTRIEVNKI
                     NRLDYYEAINSTPYYKLNPLFIKNKTTGKYTLKQKLKGYNDEMIKSLEKYLVSGNINL
                     KKMKKENLAIVYVPEISKTQKYTKTFTPGEGTPAVDIKVGDTIKVKYPNGGMDTGLYE
                     SLKDNYEYLEYEFKVGAIISYPFADNGMYSGDDSVDVITSSEYLKKLTGVDKYNLVYA
                     DLNENANKKKINNLLGKIGSEVPGTTTVDMMVEKENSDKMTSRTMIYSYGIVAIMFII
                     SVLNIINNISYNLTSRTSEFGMLRAIGISERGFKNMILYEGILYGVLSSIITIVSGLI
                     IQFKMYYMQGFVSYGLGFSIDYKIYILVVVANIIVGILATYIPSRKINKISIVEAINI
                     TE"
     gene            29629..30300
                     /locus_tag="ACOIAF_001643"
     CDS             29629..30300
                     /locus_tag="ACOIAF_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2668657.1"
                     /translation="MPILETINLGKIYGKKETSVHALKNANLKINKGEFVAIIGPSGS
                     GKSTFLHLVGGLERPSNGTIKVAGKDICCLSDKELARYRRQKVGFVFQQYNLIPVLNV
                     KENIELPLKLDNKKIDKEYIEDLINLLGLKERKNHLPNQLSGGQQQRVAIARALSAKP
                     SIILADEPTGNLDSKTTEEVMDLLKSSIKKYNQTLIIITHNENIARKADRIISIIDGE
                     LKLTL"
     gene            30327..30668
                     /locus_tag="ACOIAF_001644"
     CDS             30327..30668
                     /locus_tag="ACOIAF_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668658.1"
                     /translation="MLLILGNLNLNLDFDYRIIREENDDVDIFIDINYRSLDIDTDGS
                     NLFNSRIQFPFVRALILRLNKNNQCMTIHLLRDIDLFSAFANFEVDYTDSIVNIKNQN
                     EKVILNKSIKK"
BASE COUNT        10937 a         4783 c         3763 g        11224 t
ORIGIN      
        1 taacacaggg aaaacaattt caaaactaaa tttttaacct taaaaatttt gtataaaaaa
       61 tataacctta tatgctacat attatttcta aatgaaaaat taccataata atttaataat
      121 tacaggaggg tattgatatg aaaatactag tatttggagc acgcgattat gaagaaccag
      181 taataaaaaa atggtctgaa gaacataagg atgttcaagt ggatatttat cctgaaaaca
      241 tgactgaaga aaatgtagtt aaagctaaag ggtatgatgg tatatctata caacaaacta
      301 actatataga taatccttat atttatgaaa ctttaaaaga tgctggggtt aaagttatag
      361 cttcaagaac tgcaggggtt gacatgatac attttgattt agttaatgaa aatggactta
      421 tcgttacaaa cgttccttct tattcaccta atgcaatagc tgaattagct gttactcaag
      481 ctatgaacct tttaagaaag actcctctag taaagaaaaa agtctgtgaa ggtgattacc
      541 gttggatagc tgaacttctt ggaacagaag ttagatctat tacagttggt gttataggta
      601 caggaaaaat aggtgctact tctgcaaaat tattcaaagg cctaggagct aatgtaattg
      661 catttgacca atatccaaat agtgatttaa atgatatatt aacttacaaa gattctttag
      721 aagaccttct aaaagaagct gaccttataa cattacatac tcctttactt gaaggaacaa
      781 aacatatgat aaataaagat actctagcta taatgaagga tggagcttac atagtaaata
      841 ctggccgtgg tggtttaatt aatacagggg atttaataga agcactagag tcaggaaaaa
      901 ttagagctgc tgcccttgat acatttgaaa ctgaaggatt gttcttaaac aaaaaaatga
      961 atcctggaga attaactgac ccagaaataa ataaacttct ttctatggaa caagttatat
     1021 tcactcatca ccttggtttc ttcactagta cagcgattga aaatatagtt tattctagtt
     1081 taagcagtgc tgtagaagtt ataaaaacag gaactgctac taatagagta aattagtatt
     1141 ttataaatat attttttcat taattagcta ttatctaaca aaatagcctt taatttccca
     1201 aatataagag agtcagctca gtatatattg agtagactct cttatattat tttttatatt
     1261 aaacttgtac tcttcttatt cataccaaat taaatttata ttaacttatt ctaactactc
     1321 ctactctata cattttacct atttttactt ttctaattat atacatttga atgggtacaa
     1381 tctgtatttt acaaaaaaaa cacctaacaa actatgaaaa ttgtatgatt aatattaata
     1441 tatttataag aaaacataac aattatgtta gctctggatt tttagggagg gagtatattg
     1501 gaaaaaatga ataaagttac ttctaaaaaa actacctata ttgttacttc agcattattt
     1561 gcatccataa tatgcctgac aatagcatat atattacata ttccagttgg tggaaacaat
     1621 ggatatgttc atataggaga tgcttttatt tatctagctg caactatttt gccaacaaac
     1681 tatgctatag ctgcttctgc aattggtgct ggtctagctg atttatctac aggtgctgct
     1741 atctgggtaa taccaactat aatcataaaa ccaatacttg tattattttt cacttctaaa
     1801 agtgacaaga taataaataa gagaaatata gttgcttcgg ttgtagcagg tatagtagga
     1861 ttagttttat acatgtttgc tgaaggtata attattggaa gttttacaag tgcatttgta
     1921 atgagcttac ttggcttatt acagccaata ggaagtttta ttgtttttat aatcttaggt
     1981 atggcacttg ataaacttga ttttaaaaaa cgttatttta attaagaatt taaatttatg
     2041 gagggaaaat tatgaatatc ttatatacaa ttgaaaatag tatctatgta aatataacta
     2101 atacatgtcc atgtagttgt gttttttgta taagaaacga aaaagatgaa gttgcaaata
     2161 gtggtagttt atggctggaa catgagccaa gtgtagatga agtaaaagaa gccttcaata
     2221 aatacaattt agacgactat gatgaaatcg tattttgtgg atatggcgaa ccattaatga
     2281 gaataaatga attgatagag gtagcaaagt ttataaaaga aaaaagtagc ataaagataa
     2341 gaataaatac aaatggtcta agtgatttaa tacataacaa aaaaacagct attctgttaa
     2401 aaaatgttat agatgcagta tcaataagtc ttaatgcccc aaataaagaa gcttataata
     2461 gagttactca accaaagttt ggagaaaaat catttgatta tatgttagat tttgcaaaag
     2521 attgcaaaaa atatataaaa gaagtagctt tttcagtagt agatgaaata tctccagaag
     2581 aaatagaaga atctaaacaa ctggcaaaaa aacttgatat accattaagg gttagacata
     2641 agaattagca agtaaaatta taaaacatta attataaaac attaagaatc acctgaatgg
     2701 tctttcaggt gattcttcct cttgtattaa cattttacaa aatagatttc ataccacact
     2761 aggaatgtgt aaatcatcca aatctttctc caattatcag agtttccact aatgtagtca
     2821 ttaaaaatct cattaatctc atctatgtta aagaatttat ctgctatctc actattgaat
     2881 ttatctcgga tgtccttatt atattggtca tcagccatcc atatacgaat tggcacaata
     2941 aatcctaatt tatttctaaa agcaatctct tctggtaatg tttttgatgc agctgtacga
     3001 aaggcaatct tattctcttt tcctttcact ttatattctg atggtattct ggatgcaata
     3061 tcaaatattc ttgtatctgt aagaggcatt ctgacttcca atccaactgc agtactcatt
     3121 ttatctatat taaaataaat atctccttcc aaccatattt gaatatctac atttgacatg
     3181 ttagtaagtg tatctagttc ttctgtttct tcataaattc cctttaccac ttcaatagga
     3241 agcacatcag gattgtattt cttaagaatt tttcttttct caccctcatt cataatgttt
     3301 gtatttccaa tataaaatgt cttcaaattc tctccataac actctgcctt atggtatata
     3361 ttatatccac caaaaaactc atctgctcct tctccagagt aacagatatt tgtgttttca
     3421 gctgctgcct tgcatccaat agcaaaaacg atagcagatg catctccagt tggttgctcc
     3481 atatggtaca ttacataagg tactgacgca aaatactctt caggtgtaat tatatatctc
     3541 gaatttttac acccaagaat attagctgtc tgttgtgcta attttgattc atcaaaacgc
     3601 tcttcctcat aaccacatga acctgttttt tctatatctg acattgccaa tatataagat
     3661 gaatcaacac ctcctgatag gaataactct gcagtttcat tctctgattt tacctctggc
     3721 ataattttct gaagtgttgt atgaatttca tccgtccatt cctcaataga cttgctgtgg
     3781 tcaggatgaa attcaggttt ccaataacga ccaatacata ctgttttatt ctgatagaca
     3841 aggtaatgtc ctggcataag cttctttact ccacggaaga atgtattttc acctgctaca
     3901 tatgtgaaag acatataaat ctgtaacatg tcttcattta actctttcac aaatcctggt
     3961 tgctccataa tcttacgaat agatgaacca tagagaagct tcccattttc agtctcataa
     4021 taatagaaag gttttactcc aaactggtct cttaggcaga agagtttctt ttccacagta
     4081 tcccataatg caaaagcaaa cattccatgc atgtgatttg ccatatcata tccccatgtc
     4141 ttatatgctt ccactaaaat tgctcgttca cgctcatctc tacataatga agaatcgatt
     4201 ccaagttctt cacagagaac tttccagttt cgaatatgtc cattgtattc tagaattttt
     4261 atcatatatg tgtcttttcg tgttaaatac ttttcttcta aatttatcat atgtgtatcc
     4321 tttcgtatta aaacatttaa cttctaaatt tattaaatgt ttatatatat ttattcactt
     4381 ataatcactg aaaaatcgac ttttttcaaa gtaaattctt tttgaattag tactaatctg
     4441 ctctttatta atggcatcaa tatgattatc aagtattatc agtgttataa cactgaattt
     4501 tttattcttc ataaatattg ctccctttat attaccaact aaatataata tatcttatgt
     4561 tacttacata ttctttaaga aattctgaag attttataaa gttttttgta ttataatcaa
     4621 ttaaaaataa aattctagtt ataaagcata ttttccatta aacaaaaaaa tagttaaaat
     4681 aaattcataa ctacataaac ttattttaac tatttgtata ttttaattat aaatattaac
     4741 ttaaattttt aacaggatac actcataaaa taaacataat gaacaacaaa ataatatgtc
     4801 tcagtttctt tatacacttt accttctttc aattttctac catgtagacg tacaagataa
     4861 tattagtaat tcactctatt gctgtaattt actctattgc tgtaattcac tctattgctt
     4921 aactcgtgcg atatggacaa ccaagaaagc tatttcctca tttgttaact tttttccata
     4981 tttcttttct aaaaaatctc taacttttaa tgcaattaac atttcttttg gatttgacct
     5041 tttcatttga ttaaataata aatcatcacc actgcttaac atactatctg aaaaaaatct
     5101 ttccacaaaa aattttatat gtgttataaa tctcatataa tgcatactct ttttatctaa
     5161 ggttctattc aaagaaaaaa caaaaatatt aattatttct ccaattactt tggcatactt
     5221 aatcgtatca tatgaatttc catctgaagc catagcattt gcaaaatgga aagcaatgtt
     5281 agctgcttct tcctctggta attgaatgcc caatctctcc tctaccatat aaattgcaac
     5341 cattccaacc ttaaactcat ttggataata attttttatt tcccaaaata ctcgatttgt
     5401 aatttttatt cctttacgca tcctttcaat tgcaaagtta aaatggtctg ctaatattaa
     5461 gtataagttt ttattaaaag atgagttcaa taacttttct gcctctacta ttatttcctg
     5521 tgttatcttt aaaatgtcga caggaatatc atccattaat tctaaaaatt gttttgattt
     5581 tgtattttca acagggacaa acatttgatt atcattggaa ccacaaatat attctcctgt
     5641 ttttttacca tatccaattc ctttccctag caaaataaat tcattatcct ttgtgtcatg
     5701 aactaaaaca acgctcgagt tcaaaacttt ctttattatg tagttcattt tatttgctca
     5761 ccttctattg gtcatttaaa atctatacta ttattttcaa ttactttttt ataccaataa
     5821 aaactatctt tcttatatcg attatatgtc ccctttccat aatcatcagc atccacataa
     5881 atatatccat aacgttttga catttgtttc gttgattcac tgaccaaatc aatacaaccc
     5941 catgaagtgt atccccaaag ttcaacgcca tcttcgataa tcgcatctaa catacattta
     6001 aagtgttctt ttaaataatt aattctatat tggtcatgaa cttttttatc ttcagttaaa
     6061 atatctttag ctcctaatcc attttcaact ataaataaag gctttctgta tctatcatat
     6121 aattcaatca aagatatacg tagacctatt gaatcaattt gccaacccca ttcactcatt
     6181 tctaaataag gattttttac agctaataac gtattcccag gtgtaatatc taatccagtt
     6241 gtatcagctg cctcacaaga agacatatag taagagaatg aaataaaatc tactggatac
     6301 tttttcataa tttcatcatc atgttcttct tttactattt gaatgttatt atttttaaaa
     6361 tacgacagta aatatactgg atactcacca aaaacctgcg tatcactata tgcataaata
     6421 cttctcattt tttgttgagt tgccaaaaca tcttctggtc tacatgtata tggataatac
     6481 gttaattttg ttaacataca ccctactttt gaatttggaa taatctcatg gcaaattttt
     6541 gttgcaaggg cactagctac aaattgatga tgcatagctt gaaaaataac ttcctcaaag
     6601 tttttatcct taaaacggtc tcgaactaaa ccaccagtag tgtacggatg acgaatcata
     6661 gaatcaactt cattaaatgt aagccaatat ttcactttgt ttttatatct ttcacagata
     6721 gtttctacat acctaacaaa catattgata acttgtcgtg aataccatcc atcatagttt
     6781 aaaacgatat ttaatggtgg ttcataatgt gacatagtaa caagtggttc aattccataa
     6841 cttatcaact catcaaatac atcatcataa aactgtaaac cttcttcatt tggctcttta
     6901 tcatctccat ttggaaaaat acgagaccaa gcaatactca ttcgatatgt cttaaatccc
     6961 atttctgcca ttagagcaat atcttcttta tagtgatgat aaaaatcact accatgacgt
     7021 tttggataat ttacttcatc ttcaggatgt gctaatcctt cttctatatc ttttgttgtg
     7081 atttcattat tagatttata atctttcaca tctgcatcaa agtgtgcctt tgctacatct
     7141 gatacagacc accctttccc acctaaattc caaccacctt caaattgatt agcagctgta
     7201 gctcctcccc aaagaaatcc ttctggaaac tttctcatat ttatattcct ccctttacat
     7261 caaacaagat acttcctttt tttacattgc cataagactg gtttaacact ttataagcat
     7321 aatctgctcc attattgttt acaatcataa cagttgaatc taaattagca tgtgagattt
     7381 tgtctaaatc aaattgaatt aataaatctc cttcttttac attatcgcct gtcttaacaa
     7441 atgttttaaa tccatctcca ttcatactta cagtatttat tccaatatga atcaaaatct
     7501 cagccccatt ttttgttctt aaaccaattg catgttttgt ttcaaataac atcacaactt
     7561 ctgaatcaca aggtgcgtat aaatttccat tggtcggtat aattgcaact ccatctccca
     7621 aagtcttact tgcaaatagt tcatcattaa ccttacttaa atctattact tttccttcta
     7681 ctggagttac taagatattt acttcttttt cttcttctag ctcttcttca acaacatctc
     7741 caattgatga ctctactgta tttgtatctt ccttccaaat aatcagtgta ataataaacg
     7801 agcctaatat tgcaataaca aaaccaatac ttgcaaaaat caaattgttt ccattgtttg
     7861 ggtcaataaa catactcata ctggcaagcc ctggtccaac aattacaaat ccttttactg
     7921 caaataatcc taaaaatgta ccagaaattg cacttgagaa tacaacacct aacattgctc
     7981 ttttatgttg taaagtaata ccatataaag ctggctctgt aattcccatc aaggcagaaa
     8041 ttcctgcaga taatgctgtt tgttttaatg tttcatcttt tgttcgcaag gctactgcaa
     8101 aacaagcacc actttcagaa atattgtgag ctaatgaaga tgtcatatat aaagcttcat
     8161 aacccaactt tccaattgta ttaattgaat aaggtattag tgctttgtgc attcctgttg
     8221 caaccataaa tggaagaaaa gcagcaagta atcctacaac taagaatccc attttgttat
     8281 acaagaataa aataatggtt gtaaaaattg taccaacgtt atatcctaaa ggacctaaaa
     8341 tagttagagt aattggaaca gtaattgcca aagacatcat aggaacaaag aaagttcgaa
     8401 ttggttttgg tgaatacttg ttaaaaattt tctccattgt tgccaagaaa gacacacata
     8461 aaatagctgg gaaaacctgt gcattatagg caatgtttgg aattgtaaat ccaaatagtt
     8521 cgacaccttc acttaaagca gttgttgttg ctggaagcaa taagtatcct acagcggcaa
     8581 tagctactaa acgattacat ttcaatacat tggcagtagt tacagctacc ataagaggta
     8641 ggaaatagaa tgttgcatct gaaatagcaa ctaataatgt atatatagta gaatctgatt
     8701 ttaaaatatt aaacattgct aataacatca acagtgattt taaaatacca gctcctgcaa
     8761 tagcaaaaat cagtggttga aaaatattaa taataaattc tataaatctt gttcctaagt
     8821 ttattttttc ttcagttttt acagtactat catttgtatc taaagggcaa acttttaaaa
     8881 tttcatcata cacatcaaca acactatttc caatgacaac ttgaaattgt gccccattaa
     8941 ctatactcat aacaccttgt acttttttta aagcatcctg attgacttta cttctatcaa
     9001 tcaacttaaa gcgtagtcta gttgaacagt gttctaaagc taaaatattt tttgaaccac
     9061 caacttgttc aataatgtca atggcagttt ttttataatt catcttgtaa tctccttctc
     9121 cataattttt tatgaatggc cattcaagat gcaaaaaagg cagaacctat ataaacacct
     9181 tgaaaaatca aagcaatata ttcgatcctg cctgcattac cagtaacatg tcttctattt
     9241 atactatttt gtattattat attaacattt gcctaaaaga attgtcaata cttttttaaa
     9301 ttttctgata ctaactaata gcacctttat aattcatttt ttatgtcaat taaagtaaca
     9361 gtagccaccc accaattagt atcaaaattg aacctggtat tttttctttt atattttctt
     9421 tgaatataaa atgagcaagt attaaagtta ttatcataga tacttgagaa aatggctctg
     9481 ctatactaag ttctagatta tttaaagcaa ttaataatgc taaataagaa tatccattaa
     9541 tagcaccact taaaattgaa agtccctttt tattagtaaa aatctcacaa attatttttt
     9601 gtttcttttt aaatgctaaa aagataaata aatttaaacc tacgaaaaaa tacagtatag
     9661 ttgaatataa tataggagat acttgtacaa aaaaagcctt atccaatact cttcctactg
     9721 actgcaaaaa tatgtataaa aacattaatc tacatggtaa attgttaaaa attacagaga
     9781 cattctttaa aaaagagctt acttttttta atataaacac tccaccaatc ataattatga
     9841 ttcctgcaac ttttgctaaa cttaaacctt cacctaaaaa tataactgat aaaagcagta
     9901 aaaacaatga atttaaacta tttattggag taacaagtga aacttcccca gttgctaaag
     9961 acgttacata cgctactgaa ccaattgaat agactaaact acttatataa catggtatta
    10021 aaaacgacca atcactaact ctatccatta tgctaaatgg aagtaaacat actcccccta
    10081 taaagaaaaa tagaaatgat gaagctatat ttttataaat atctccttct tcatcaccta
    10141 ataatcttac aactactctt tcaaaagcta atagcactac tctaattaat aatgcaaaat
    10201 aagataatat cattttgacc tcgcttaaaa attaatccga cttattgtta tattaactgt
    10261 ataaaactac catataggta agtcttttgg attttttaat attttatgtt taaatacagg
    10321 tacattaagt ttttcaccta gtttacggca ttgctcttta taggtacctt ttaatttatc
    10381 atgaaaaact atacaatcaa ttcctccttc aatactattt atacactttt ctagtttctc
    10441 aaatccagat tcatcatcat acattccact tacaatccat tgatttggca atacatgtcc
    10501 aattgatatg ttatattctt tattttttat aatatctgag aaagatttct caaaatgtac
    10561 ccctccaaca cataacaaag tcttcaaagg ttcatcacaa tcaaatactc taattaatga
    10621 ctttgccaat acctgtattg ctatactatt attaaaacta tcactactac ttcctatctc
    10681 aatatccaac ataggtacag aatattctgt aattaattta gattcttcac catatgttgt
    10741 tccagaccag tgagttgctt ctgttaatgt agtaaattca tcaagtagat tttcattttt
    10801 tatatcctca atagcattga ttagattttt aaagtatctt ggatttgaat ttccaaattc
    10861 tcctgttgga acatctccaa ttgtatgagc tgttaggata tgttctggag cattttctcc
    10921 ctcatgccaa tttacaactc ctgcaaaatc ataatctctg aaatgtctat ttaaaattgg
    10981 aagataatat ttataattat gacttattac atcttttgtt tttacaaaat gaaataagtt
    11041 attgttttta tccttaaact ctaatacaaa attatcatca ataacaatat cagtttgatt
    11101 taaatccatt atttcttcac aacactttat cacattactg gctactgggt ctttctttaa
    11161 atcactgcaa aagaaaaaaa ctgctttttt attattcatc cataaatcac actcctataa
    11221 cttaaatata atatatattc atacctaaaa tcatctaaat atcactctaa ttccattaaa
    11281 ttatttatat ataatatact accatactca ttttagctat acctttgcct gagtttctat
    11341 atatatgtga tacatcagct ttaaatctta tagaatttcc actagataca atgtaatctt
    11401 ctccatttat tttaatggtt acttcgccac taaaaacagt tataaactct tgtgttcctg
    11461 gaagttcagc atttgaatct aaatttccac cttcttctat ttcaatcata aatatctcaa
    11521 atcttctttt ggagtcaaat ggaaatattg gataacttat atactttcca ttatcttcaa
    11581 taaaatggtc tatatcactt ttttgaatca attctatgtc actttcctgc ctctccatca
    11641 atgatgtaaa tgagattttt agcccattag ttattttctt caaagtagat atagtaggat
    11701 ttacctcaca cttttcaatt tgagatagca tactcttact tacaccacta agttgtgcaa
    11761 tcttgtctaa actaagatgt ttttctttcc ttatcttttt taaattttca gcgattattt
    11821 catttaaact ttgcataaat accccctaat acatactaat ataagataaa ttattttgat
    11881 tattaaataa acttagtaat ttaatagaaa ctttttatcc attataatgt acattatgtt
    11941 taatatacat tacttttttt agaatgtaaa tagttttctg aaattttgtg aaaatgaata
    12001 aataaaaaac tagtaaccta tatttttaca aggttactag cttttattat aataatcttt
    12061 cattttaact ataaaactta gatgcaccta taagctgtct cttagaaaca atcttagcta
    12121 ggtttgctaa tgaagaaatt tttatatctt taaaagaaac taaaaataca aacttaaaat
    12181 ctgtaaattc ttgatttata ttgtatatat caaaataaca tctcaaatta tcaaataagt
    12241 aattatcctg taagtctgct tctatctcat cagtagcatc ttttaaatac gctcttacat
    12301 cttcaaataa aacactgaaa taagactcat ctatttcttc acctaactct tccaatattt
    12361 cttctatatc taaatcatcc atatcaaaag aatttcctgt caactggcaa tcaaatacca
    12421 atatattttc ttgatttata gtatttgata tatcttgtac atattcgaag tctggacgta
    12481 attctaaaat gtcttttatt tcactgctct ttacatggat ttttcctttt ctattttcaa
    12541 tttttaaatc tatcatatgc tccccctttc aatctacaaa tctttaaata ccttgtttta
    12601 taatcttttc aatatctatc caattactaa ctctaactat attttgatgt ttaacatctt
    12661 tattatagtt agtatccaga agtattactt taacaccatc atcagctagt tgcactgagt
    12721 ttaaaggatt atcttctata aatatgtcac aactcagttc tcttgccttt tcaactttat
    12781 aatcacttcc taataagtaa acatctatat ctgaaaatcc ttgtcttgat agccaatttt
    12841 tagttacatc agttaaacac tcatgtctag ccgttacaaa agataaatta tgtcctttag
    12901 ataatgtgct aattacctct gtagcaccct ctacaatctt tgcttcatca tatgagtgta
    12961 tatacttatt attaaactcc gcatacacat ctcttattcc cgaaccatac aattcttccc
    13021 aattatgtgt tatacactct gcttcagtta tatttttatt aaagatatca tttaggtatg
    13081 gaagaaaatg ataagggctt gttattgttc catctatatc aatacatata ttcaatttgt
    13141 tcatttttac ctccatgtaa catttattga tttgattaat caaatattat agcacagctt
    13201 taattataaa tcaaaactct ggatatgact atacctatat aataccaaaa agcttgcata
    13261 tttaagcaag cttttcgtaa attttacatt tttaactaat atataagttt ttctaaggtt
    13321 ggttccactc caaattgtga tttcagttct ttatattctt ttaatgcttt gtcattattc
    13381 gttttagttt ttatcgctct tatcattaaa ttttttggtg tatcaagtgg agatatgtat
    13441 tctactacag aagtatcata tccattgccc tccaataaca atgttcttaa accatcagtt
    13501 attaaatctg caaatctagc tttaaataca ccatgtttta atattggttc catagcttca
    13561 tatctatatt gccctaataa ctccttatga caacatggta ctactactat agcttcagat
    13621 tttgctctaa ttcctagacc tattgccata tctgtagctg tatcacaagc atgtagactt
    13681 attacaaggt caacatccct attaggtgta taattagtca aatcctcttt tataaatgac
    13741 atatttttat aacccaagtt tttagccata ttttttgatg cttctataac tacatctgaa
    13801 taatctattc ctataaaata acagtttttc ttcaatactt cttttatata aaaatttaag
    13861 acaaagctta agtaagattt tccacaagca caatccaaga tagttataca gtctttatcc
    13921 tttatttctt ttaaaatact atctatcaat tcaacaaaat ggtctatctg attgtatttt
    13981 cttattttat cattttttat cttaccatct tttgtaagta ctccaatttc ttttaaaagt
    14041 gcatttgctt gtcctacttt tacataataa tctctgtttt ttatttgaga tgccgtaaat
    14101 tcatctattt taggtacttc ttccttattg tctaaatatt ttacatttac cttcttattg
    14161 tctccttcaa ttacaacaat agttcctcgc tcaacatatt taaatactaa tccatcataa
    14221 ttttctgcct ctttagaaat attcttaagc aactcattaa acgtatataa atctgatttt
    14281 cctttaaaat taaatttata tttattatct tcctctattg caattccttt aaactctttt
    14341 cttccagatt taaaaatcac ctctaactca ataaaaaaat ccctattatc aaggtttctt
    14401 tgagtaatcc ccataaaaaa catatttagt tttcctatac tctgtttatt catcataatc
    14461 tcccttttaa cttatatcct taaatcttat taattaatat tatttgtttc tgaattattt
    14521 atttcttctt ttaaatcgtt caatctattt ttaaaacttt ctatctgctt atctgataaa
    14581 tcaagatatt ctgttatctt ccctatatat ttagggcttt tagtatttat taatttcaaa
    14641 gcactttcca tagatttttc tctaagctca tatgctttct ttacgtcatc tctagcatta
    14701 ctcaatgttt ctgtatactt ctccttagaa agttctggaa tctgcatatt ttcatattct
    14761 tttacaactt ctttacactt gtttctggca tttgtaacat ctttttttat actagataat
    14821 ttagttatat tttttgctat atccttagtg taaggtatac attctctcat tggaactaaa
    14881 gctgctcttt ctatattata aatttctttc ttaaaaattc ctatactttc catatctatt
    14941 ttttttatat cttcactatt tttttcttta cttgtatatt tattattttc ttttgtatta
    15001 tcttcttgtt tttctgattt attattcact gaaacattac tatcagaatt attcttttct
    15061 tttatatcat tacttccttc ttcattactt tctttatttt gtatatttct atcttgtttg
    15121 ttgtttaaat ttttattatt tgatatttct tcattctttt tattatcctg ttttaaatca
    15181 gtactttctt tttccttaaa atttgataca tacttttcat catatgtctc atcagaaata
    15241 taattgtgat aatacataga ctcttttatt ttattaagtc cactcatata aatattactt
    15301 atcattattg ttgtagctaa taccacataa atactactgt agatacaaga cctcttaaaa
    15361 ttcttgtaat ttctgaatat cactactaaa ggaataagat ataaaaatat aaaaactaat
    15421 atccacctca taagtcgtgt ctcctttctc aaaaccgaaa tccctcaaat atttattttt
    15481 aatcatgtat cttatagtat ttaccaattt atgtttttta atcgtgaact tttattaaat
    15541 taaattctgt atagtcacaa gatgctaatg tatattctac tccatttcga atccccttca
    15601 atcccatttt aaaagattct ttaccatgca agtgaccata aatcaccttt tcaacattat
    15661 attcttcaaa taactttgta aataaagatt cttccaattt atcattcgta ggaggataat
    15721 gagtaatcac aattattttt gagtgaccac ttttacgagc agattctaat gatagtctaa
    15781 gtctatgttc ttctctttta taaacttttt catcagtctc atcaaattta acatcattag
    15841 gacatatcca gcccctacct ccacaaattg cataatcttt atattcataa aaatttgtct
    15901 gaataaaacg catgtcttca tatagcttat ttaaacttgt aacggttgtc caccagtaat
    15961 catgatttcc ctttataagt attttctgac ctggaagttt gcttattata tctaaatctt
    16021 ttttagcttc ggataaatta attccccaag atgtatctcc tagtactaag accatatctt
    16081 cttcctcaac aacttctttc caattatcta taattttctt ttcatgacca tcccaattac
    16141 ttccaaagat attcattggc ttattgactg atgttgaaaa atgtaaatca cctatagcat
    16201 acaaactcat aatatcattc ctttcttaat tacactaaat aggctgtctc tttttagaaa
    16261 ttattttcca ttaagaggca gccccattaa tatttagcaa attttctaca agtagttttt
    16321 actgaactac tttatcatct accttagtat ccttatattg ctctaataat atatctattt
    16381 cttgttttaa ttccaaaatt tcagttatat ttttatctgt ttcagttcta tcaagttttc
    16441 tttggtactg catagcttta tgtgtatcac ccaagtcata taactcttgt attctattta
    16501 gagtatctct aatagctata gacttcattt ggtacatagc ctgagctttc attatgtcct
    16561 ctcttatttc actcatttct tggtctaaaa gaaaggcagc atcagatgct cttctaagcc
    16621 tttcagcaag agtctcaggt atgtaatggt catatttata ttttaatgaa tgttctattg
    16681 tagcccagaa attcattgca agagttctta tttgtatttc acatataatt tcctttgaac
    16741 ccgcaatgga gtttataggg tattttatta taacatgata gcttctataa ccactatctt
    16801 tgtaatttgt aatataatct ttttctccta ttatagtcat atcatttctt acttttatca
    16861 aatcaactat agcatatata tcctcaacaa actggcacat tattcttata ccagcaatat
    16921 catcaatttc agtttctata tcttttgcat ttatcctttt taacttagat attatggaag
    16981 atattttttt tgttctacct gtgacaaact ctatgggaga atattcacct tttgctaaat
    17041 attcttttct tatattttta aattttattt tcaattcctc tactgcatgt tcataaggtg
    17101 ctagtatctc attccatttt tcgtattcca tattgctcac cctttatttg ttttttatga
    17161 ccttctttat tttatcataa tttattttgt ttttgtatag taaagcaatt ctccattttc
    17221 caatatatac cctatttctt ctaaatcaac taagtaactt atgacagata ctaacgaaga
    17281 tttaaaaaaa tgatactctt tataattatt gcttaaatta ttgttattta taatgttttt
    17341 taataaaatt tctaaagatt tcggttctac aagcaactct cttatttgat ataaatattt
    17401 ttttaaagca ttcaaatgtt tttctattaa tttataagaa tcttctttcg atataacttg
    17461 cttgccatgc cctaaaacta agtactcaaa atctatattt tttaattttt ctaatgaatt
    17521 tatttgttct tgtatatcat atagaaacaa gaaatcgtat tttgataaaa tttcatctcc
    17581 tatcaataaa tcgccaacaa aaatcacatt atccttcgtc aaaacagcta tactaccagg
    17641 tgtatgaccc tgaaattcaa ttatatcaaa tatttcattg tttaattcaa tttttccagc
    17701 ttctatagat ttatctattt ttatatcatc caacatttta ttttttaaga tattatccat
    17761 aaatacattg ctctttcccc caacaatata ctttgaaaac aattctggat tttctatata
    17821 taatttatcg tattcagatg acattataca cacatcttta taatattctt taaattgatt
    17881 acaagctcca tagtggtcat catgaccatg agtatttata atatatctaa cctgtatatt
    17941 attttcatct aatactttat ttactctcct aggtcttgaa ccacataacc ctgggtctat
    18001 cataagtgct gtgttatctt caaatacata tattcctgta ttcgtgccac cttttataaa
    18061 aaaggtattt ccttttactt ttgtaagatt caccttcttc acctcatttt gacaatattt
    18121 atgatacaat ttattactta ctaatataaa actatacaag aacaatcctt tttattctat
    18181 ctatagttct ttttcttatt ttgtattctt tagatatcct tattttcaca ttatatgcta
    18241 aatttctata aagtaatgac tctataaaat attcaactat acctctatcc ctaaagtcta
    18301 tatctatatt tccacttgaa taaatatttg agtctaatat cataagctca tcaaaaaagc
    18361 ttaatctctt agatttactt aaataatctt tatttggaat aaatctatca caataatcta
    18421 tcctgtaatt tactacttca tcatttattt caaatccata ttgctttttc attaactctc
    18481 tatagttatt atttttctca ataacattat ctaaattttt attataatcc tcttttttcc
    18541 aatgtccaaa catcctacaa tttaatggtc taacttcata tattaaacat ctattattag
    18601 tatctttaaa tggacatggg ttctttcttg tatactcctc aaaataataa tctaatattt
    18661 tacttataca gctttttttt aaatcaactc tatcttcaag ataacaaaat atattcaaaa
    18721 actcaattaa atttatacct actgactcca tacaacaatt tccacatcca gaacaatttc
    18781 ctactggcaa ggacttatat acatcattta gtttgtcaaa gagcttattt ttaatggaat
    18841 aatctataca atttaaaata tctttatttt ttatactagt cattattatt actccttgtt
    18901 ataattatta agttaataca aacaattatt ataacatatt ttttaaattt aatcgcatat
    18961 ggaggtattt tatgaataga tatacaaaaa taattaatat gatgggaagt tattacacaa
    19021 aagactttga aaaagaaaaa aagaatgtaa taaaagttag agaagttaaa gaagatacag
    19081 ttagaaaatt tttcttacaa ggtgactgtg aagttcttgt agtatttgaa gacactggga
    19141 aagaaatact tattgatgac ttttctcctg aagaagatat caaaaaatat ttaggagcaa
    19201 aatttataaa taaaaaaaga tagttatcaa aaataattct aaaattaaaa tttaataaat
    19261 gtacagttat aattctatat aattatagtt aaaagttatc ttttattgct tttttagact
    19321 ttaagatagc tttttttcta ttttttgtaa aataattcca taattcacat taataattca
    19381 caaaatgata taatataaca aatagataaa ttttaaattt ctaaatacat aattaatatt
    19441 ttgagaggtg attttatgca aatttatttt agagttaagg ctgttggcaa acgcaaacct
    19501 atgctagaac ttaccccttt tgatgtatct gacaacatca attgcctaaa agaagctatc
    19561 tctgaaattg tatctaaaaa tgttcttgaa tataatgaga aaatcccaga aaaatcaata
    19621 ataaacttct taactaatga agaaattgaa tctcaagctg atattggaaa agttggattt
    19681 ggctctattt acaatgagaa taaacaatct gtagaaaagg caatagaagt agcgttacag
    19741 gcatttgaag atggaatcta caaagtgctt ataaatgaag atgttattga aaaactcgat
    19801 tcctctataa atctgaaaag tggagatata tttactttta taagacttac cttgcttgct
    19861 ggaagaatgt tttaaaataa ataatttgga ggtacataaa tgttagaacg tactagagta
    19921 gaaaaaaatc ctaaaataat tgaaaatttt aaaaataatt ttactaaatc ttcttcaaaa
    19981 cagtactacg acttagcaaa gtctctttgg gattgcatag aaaatagttc atactattat
    20041 agagaacctg aagacaaatt cttaaatatg ataaagaaat actacaattg ccactactac
    20101 gaaattaaaa atatggacga gctcctttct ggacctttaa aaaatgcatt agaattaata
    20161 tttacttctg aacaagtaga agaactttgc caattaatta aaagaaagat ggattataca
    20221 tattcaggag gatattatcg taaaggttat cgctcaaata aacttgtgga ttattttaga
    20281 ggtattataa ggtttatttc aaactatgtt gatttaattt attatgattt ttcagttata
    20341 gattatctta ctcttccaaa taaggtcttg tactatgact ctataatgag tgataaaata
    20401 gcactagaaa tagacaaagg aaatcaaaaa gtaataagtg ctttagaaga agtaatattt
    20461 ggagaaaatc aaactgctct acttacaaga caaatgataa ttggtatcat gaagagtcat
    20521 agtgataaat gctatgaaat gctttctaaa ctccttttgg cagccaaaca acaagaagga
    20581 cttagacagc aaattgtaga gagtttagat gagggtacaa tttctgcatc aatatacatg
    20641 ttaaaaacta ttttggacaa caatttagag cgatttagtt ctgttataag agccttggat
    20701 acatggacag gacttggttt ttcagaccaa aaacctggaa taatacgtaa atgtttagaa
    20761 ctttcatata aatcattgac tgatgaaaag ttccgtatag aatcaattaa tagcaatgat
    20821 aatcttgaaa tttattttgc cttgtgggct actgcaactt acaacatata tgactcttac
    20881 tctcttattg aaaacttact ttcttgtcag gaaaaatata aaaaacttgt agctctttac
    20941 tttttatcgc atacagaaag tacatatttt aaacattcta tagcatctaa aaacattaag
    21001 ggtgatgatt tagagatact agcatgggta attacaaatt tctatgtaga aggttctgtt
    21061 ggctactact atagttggca aaacagagaa actaattttg acttgtcaaa cactcctatt
    21121 gacaaagact ttgatgctag aaaagaacaa tttgaaagtt taaaacatac tttagaaatt
    21181 ttaaattcaa agacaaaatc attttcagga agtgtatttc catggacttg gattgaaatt
    21241 tcttctcatg aaatacttaa atgtatgatg actttaacag catacgatat gaatgctcaa
    21301 tttatagatg tgcttatgga aaattgtcat cttatgagtt cagatttaag agcttcactt
    21361 atagtcaatt ttgtaaatga accaaagaca gatagacaac gtgaattttt attggaatgt
    21421 ttaagtgata agagtgtttc caatagagaa acggtattga gtctacttag aaagcttgag
    21481 ctaacagatg tagaattaga taagataacc aatctattgt ctttaaagac aagctctatt
    21541 agacaaacag taataaaaat tttacttgaa caagatgatt caaacttgga aaatattgct
    21601 tcttcactga taaaatcttc aaattctcaa aaatgccttg gaggtcttga aattgtaaac
    21661 ttattaaaag acaatactgc tagacaagaa ctatataata agcttgtttt acttatagaa
    21721 gatattgata aatctaaaaa gaatacatct caagttcaaa tactaattga taatttgtta
    21781 ggtaaagcaa aaaatgatta ctgcaaagaa aatggttttg gaatatataa ccatgaaaac
    21841 aaagttcaaa ttccagaaca atttgatttt aaagatgatt ctaatagctc atcaaagctt
    21901 aataaaatta tttcaaatat aagtttatct actcgtgagg attctgtttc tgtagcaaca
    21961 aaaaaagaag caaaaaaaat tctcgatacg gatataaata aaataataaa agtttatgaa
    22021 aaatttgatg agcttattac tgaaaatgca gattatgaat atgaagttga aagatggtca
    22081 ggtaatgtag acaaagtact tttaggttat tctacttata acttgactcc tattaaatat
    22141 aaagtttcta atgatactct tgataattat ccattagcag aactttggag agaaactagt
    22201 aaaaaacttg acttagatta caaattaata cttgatatct tattttatag tataagtaca
    22261 tgtggatata atgaaacaga aaaaagttgg ttttcatcta tatattcaaa tgtccttgac
    22321 tctaaaaaac aaaataaaat atatacaaaa cttgaaaaac ttaaatttaa tggacttaca
    22381 agaaaacttt taagagtttt actaaatgag tttgaccata aagaaatatt taactattgt
    22441 ttagaaatat actataaact tagaaactta ttgactatag aacaatgtaa aaaacaaaga
    22501 gttgaattta ctaaaaacta ttacagacaa aatgaatact gccctgcttt aaatgcccct
    22561 attttaaact actggcttga taaagcacat tcgtttattt atgatgatat ttcttttact
    22621 aagtttttct acactacgtt ttcaaattat cagttgctag aatataagtt tcctttctat
    22681 actagcataa atgtgtttgc tcgtgcacat gaattaggat taatgactga agagcaagtt
    22741 aatagagaaa tgataagtgg atacaacgca tcaaatataa taagaacatt gacaaatcct
    22801 agatgggctg gcgacacatt agaaaagtac ccttggctta aagataattt aaaaactgtt
    22861 acaaaccgaa ttatagaaat tgaatcacgt agaggtgata tgccaacaga agtgtctcat
    22921 ctagctaata atatacaata ctttgaaggt ataaattatt ttgtaaatat tttggtcgct
    22981 cttggtaaag aaactttcca gagaggatat tcatatggta gcaattattc aaaaaaagat
    23041 gttttaagtt ctctacttaa aaattgctat cctactaaag aagatgattc aaaaaaatta
    23101 aaaagtttac ttaaaaatac cgacattact gagttaagat tgatagaatc tgctatgtat
    23161 gcccctcaat gggtaaacat aattgaaaaa tatttaaact ggaagggtct gaaaagtaca
    23221 atttggtact tccatgcaca tataaatgaa ggcttctctg cacaaaaaga atcagaggtt
    23281 gctctattct cccctatttc accacgtagt ttcattgatg gtgcatttga tgtagattgg
    23341 tttttagatt catataaaac tattggggaa gaaagattca atatacttta caagtcagct
    23401 aaatatataa ctagtggtgg taactctcat agacgtgcac aactctatgc tgatgctaca
    23461 cttggtaagc ttgataaaga catgttgatg aaagaaattg aaacaaatag aaatcaagat
    23521 aaattgagaa gtttctcttt acttcctatg aaaaaaggag acctaaaaga agcaactttc
    23581 cgttatgaat ttattcaaaa gtttgctaaa gaaagcaagc aatttggtgc acaacgtcgt
    23641 gaaagtgaat caaaagcttg tactatagcg ctagaaaatc tagctagaag tgctggattt
    23701 ggagatgtaa atcgtatgat gtgggctctt gagactgaaa aactaaatga agttgctcat
    23761 ctatttaaac caaaagatat tgatggtact tcaatttgga tagatgttga tgaaaacgga
    23821 aaagcatcta ctaaagttca aaaaggcaac aagtctttaa agagtatacc aaaagctatt
    23881 gctaaacatc catatgtact tgaacttaaa tctgtccaaa aagatttaaa gagtcagtat
    23941 tctcgtgcaa aacagagttt agaacaagct atgaaaaatg aaatagtatt tacagttaaa
    24001 gaaattgcca atatctataa aaatccagtt atctgccctt tgattaaaaa tctagtatgg
    24061 gtatgtgatg ataatattgg atatgtttat aatgataagt ctaagttctt tatttgcaat
    24121 atagaaggtg aaacttataa gttgacggca aaaaataaat taaaacttgc acatccagtt
    24181 catttgtttg aatctggtaa gtggagtgag ttccaacatg atttatttag taagcaaatt
    24241 gttcaaccat ttaagcaagt attccgtgag tattatagaa taaacagtga tgaattatct
    24301 gaaaaaacta tatctcgcag atatgctggt caccaaattc agccaaagaa aactgtagca
    24361 cttcttagaa atcagctttg gactgtcgat tatgaagagg gattacagaa agtattttat
    24421 aaagaaaata tagtagcaaa gatgtttgca cttgcagatt ggttttcacc agcagatata
    24481 gagcctccta ctattgaaac agtagaattt tttgatagaa aaacttataa aagtttagaa
    24541 ttagcttcta tacctccaat tctattctca gaagttatgc gtgatataga ccttgttgta
    24601 agtgtcgctc atgctggtgg tgttgaccct gaaactagcc attctactgt agagatgcgt
    24661 atagcaattg ctaaagaact tttattatta ttaaaaatta acaatgtcaa ttttgaaggc
    24721 tctcatgctc ttatagcagg taatttaggt gagtattctg tgcatatggg ttctggtatt
    24781 gtacataaaa aagggatggg tgctttgaat atacttccta tacattctca aagtagaggt
    24841 aagatattcc ttccattcgc tgatgaagac cctaaaactg ctgagattat gtctaagata
    24901 ctattacttg ctgaagatag taagattaaa gaccctagta tcttaagtaa tttataagta
    24961 taaaaactat ctctaaaata tattggactt tcttaaaaaa attagaaagt ccaatatata
    25021 ctatactgta atttatatag attaaatcat aattacaaat aatttttata taaacttctc
    25081 ttatacatta tctttacggt acttattata tcacacaaaa agtatttttc ttctatcttc
    25141 actagaatct tttaacccta taaaatctat ataaaaaatc tttttctaga gatttgttta
    25201 atatggcatt ttcttgaaaa tttataaacc ttatctctaa tgaagattta tttataggta
    25261 taaccttatg tataaatctt agatttacta tgtattgtct atgggtcata aagaaattag
    25321 gaggtaggcg tttttttaaa tcatcaaagt tctcataaaa ctttattata tcttcatctt
    25381 tagtgtgtag attaattgct cttgaatttt tctcaaacat aactatatca tcaaaattaa
    25441 tcatatgtat ctgcttcctt attttatata caaaaagggt gtcttctata aaattattat
    25501 tagcaatctt atgggagtta atatggtcta ttgctatgtt aattgaatca agtatttttt
    25561 cttttttaaa tggtttaact acaaaatcta taggatgcac tttaaagctt tcaagactat
    25621 aatcagcgaa acccgtcaca aatactatat gtatatcctt atctatagaa agaatttctt
    25681 cagcagaatc aatcccattt ttatatggca tatcaatgtc aataatcaat aaatctattt
    25741 tctcattttt tataaattca attgcctcta taccatcctt aactttttta acataatcta
    25801 catatccaga ctctttagct atatttgcta tatcatctct tacaatctta tcatcatcac
    25861 aaatcaaaac ttttatcata gacttttctc ctcctaacta ataatatttt gggattaaga
    25921 ttacaaattc agttaaaaaa tcatcactat ttacaagtat actataatca tacttatcta
    25981 atatattttt aactaaatgt agcccatatc ccctatcttg tccttcttta gtactaaatc
    26041 cattccaaaa tatactttca acattatgta ttttaggacc attatttttt atagctataa
    26101 tatactgaaa ttcatcttca tatgcatgta atcttatata cttcttttct ttttttatag
    26161 tagacaaaga ctctattgca ttatctatta tgtttaatag tactgaactc atatcattag
    26221 gttcaatctc taaattatcc ataaaatctt gaatgtagac atcaaaagat atatcctttt
    26281 caagacattc ctcgtatttt aatgaagcta tagcatctaa ataagatact tttccatacc
    26341 ttgaattttt tgatttaaag ctatttgata tattcattat atattctttg gcatcttcat
    26401 ctagcccagt atttaataga gaatacacta cttgcaaatg tttcaaatag tcatgttttt
    26461 gacttcttaa tttgataatt atattatcct tatgatttat tttactatct agtttttgta
    26521 atttatattc ctgttcatta ttattattta tcaatcttat aactttaaag cctgtataaa
    26581 aacttaaaat aattaaaacc aaaaaaagta taatatttaa tactccttca agtaaaaaac
    26641 tatatcctaa ccttaattgt atatatttag tgccctccat aaataaaatc aatgcaaaac
    26701 acgatataaa aaatgttaat atgcctcttt ttaatatgtc taattctttg taaaatttat
    26761 acttattcat tataagattg ctaaatataa acaaagttag tattactata ggaaatacaa
    26821 aattgttatc catgattccc ctcccctcta caatttatat agggattata tcattttaaa
    26881 aaaattatgt aaaaagttaa aatttacata tatattaatt aaacttgtaa aaatacagat
    26941 gaatttgtac aattaactac ttaaaacttg aaaatactta ctttgctata tattttagat
    27001 aaatatataa ttgatttagt taagaaagga agtggtaaat atgaaagata atttgaaaat
    27061 agtactaagg tatataaaaa gttataaagc taggtcttta gcaattgttg tgagtataat
    27121 tcttgctaca tcactcgttg ttggtattgg taccctctca agaagtgctc agcaagcaga
    27181 tgtagataga ttgaaaagag agcttggaac tgaccatgta tactttaaaa atataaagaa
    27241 aaatcaatta aagtccataa aatctagcaa agatattaaa aatctgggta taacatctta
    27301 ctatgcatct acagatgtta atgaaaaatt acctataaac atactatatg caaataaaaa
    27361 ttatttgact agtcattcta aacttataaa aggtcgattt ccaaaagcta gcaatgaggt
    27421 ggtagtcgag aaatggattt taaatagtat gggcttaaaa ccagagataa atcaaaacct
    27481 tacttttaaa ttatatcaaa aagaaaatcc agaaactttt aaagttgttg gaatccttga
    27541 agatagattt atagagaaaa aaaggggaca atgtgaaatt ttttctaatc tcaatgaatc
    27601 agaactgaat aattttacaa cttatattga atttaatgaa ggcagtgata taaatacaaa
    27661 catagacaat ataataaaaa atgctatgtt agatgagaaa agtataggaa gaaactcaat
    27721 gcttatagaa tctactatga aaaacggtac tttagataat tcaagtaaat acattgcaat
    27781 tgctatgagt ctatttgctg gtattgtaat atatagtatc tatgttatat caatatacca
    27841 aagagtacag gaatatggca tcttaagagc tattggttcc accaacttta ggattttcaa
    27901 acttatgctt tatgaacttt tcatacttgc tctaatagca atgcctatag gtatatgtat
    27961 aggtatggga ggtgcacaaa tatttaatag gtctgtagga aatattcaat ttgaaggaaa
    28021 tataaatgtt acaccttttg ttattccaga taaaataatt ctcttatcaa ttgcatgtac
    28081 aatactaata atgctaatta taagtttctt tacatactta aaaataagaa gaatttctcc
    28141 tattgatgct atcagaaaaa cttttggatc tgataaaaat gtaaccaaag ttaattattt
    28201 aatatcaaaa ttaactttaa atatgtcagt aacaaaatct atatctgcga aaaatatctt
    28261 tagaaataaa aaggggttca tgataattgt attatctatg agtataggag gtataatcgt
    28321 cataaaagac aattataaat attctttttt agatgtgcaa aacaaatccg tacaagaaca
    28381 aacatatatg aatgcagatt ttattctatc aaatgtttat ctcaatgaaa ctggtaacaa
    28441 tacttctttt aaagatataa aagggtttag tgatagtcag atagataaaa taaaaaatat
    28501 agatggaatt gacaaagtca aaactgctag tatcttaaac acaagaatag aggtaaataa
    28561 aataaataga ttagattact acgaagctat aaattcaact ccttattata agctaaatcc
    28621 tctgttcata aaaaataaga ctactggcaa atatacacta aagcaaaagc taaaaggtta
    28681 taatgatgag atgataaagt cattagaaaa atacttagta agtggaaata taaacttgaa
    28741 aaaaatgaaa aaagaaaatt tagctattgt atatgtacca gaaatttcta aaacacagaa
    28801 atacacaaag acttttactc ctggtgaagg gacaccagct gtagatataa aagtaggtga
    28861 tactataaaa gtcaaatatc caaatggtgg gatggataca gggctttatg aaagtctaaa
    28921 agataattat gaatacttag aatatgaatt taaagttgga gctattataa gttatccttt
    28981 tgcagataat ggtatgtact ctggagatga tagtgtagat gtaataacga gtagcgaata
    29041 cctaaaaaaa ttaactggtg tagataagta taacttagtt tatgcagacc ttaatgaaaa
    29101 tgcaaacaag aaaaaaataa ataacctttt aggtaaaatt ggaagtgaag ttcctggaac
    29161 tacaacagta gatatgatgg tggaaaaaga aaattctgat aagatgacat ctagaactat
    29221 gatttatagt tatggcatag tagcaataat gttcataatc agtgtactta acataataaa
    29281 caacataagt tataacttaa cttcaagaac tagtgaattt ggaatgttaa gagctattgg
    29341 tatctctgag agaggattta aaaatatgat actttatgaa ggaatattat atggtgtact
    29401 ctcaagtata attactatcg tgtcaggact tataatacaa tttaaaatgt attatatgca
    29461 gggctttgta tcatatggat tgggattttc aatagattac aaaatatata tactagttgt
    29521 tgtagcaaat ataattgtag gtattctagc tacatatatt ccatcaagaa agataaataa
    29581 aattagtata gttgaagcta taaacattac agaataggag gaataattat gcctatattg
    29641 gaaactatta acttaggtaa gatttatgga aaaaaggaaa catctgtaca tgcactaaag
    29701 aatgcaaatt taaaaataaa taaaggtgaa tttgttgcaa taattgggcc aagtggaagt
    29761 gggaaaagta catttctaca tttagtaggt ggtcttgaga gaccaagtaa tggtactata
    29821 aaagttgctg gtaaagatat atgttgcctt tcagataaag aactagcaag atatagaaga
    29881 caaaaagtag gatttgtctt tcaacagtat aacttaattc ctgtattaaa tgtaaaggaa
    29941 aatatagaat taccactaaa gttagacaat aaaaaaatag ataaagaata tattgaagac
    30001 cttataaatt tattggggct taaggaaagg aaaaatcatc ttcccaacca attatctggt
    30061 ggtcaacagc aaagagttgc tattgcacgt gctttatctg caaaaccaag tataatccta
    30121 gcagatgaac caacaggaaa tttagacagt aaaactacag aagaagtcat ggatttacta
    30181 aaaagttcta taaaaaaata taaccaaacc cttataataa taacacataa tgaaaatatt
    30241 gctagaaaag cagatagaat tatatctata atagatggag agcttaaatt aacactctaa
    30301 aactatttat agaaaagagg atttaaatgt tattgatttt aggtaattta aatttaaact
    30361 tggattttga ctacagaata ataagagaag aaaatgatga tgtagatata tttatagata
    30421 ttaattatcg tagcttagat atagatactg atggttctaa cttatttaat tctagaatac
    30481 aatttccatt tgtaagagca ttgattctaa ggctaaataa gaacaatcaa tgtatgacaa
    30541 ttcatttgtt aagagatata gatttattct ctgcttttgc caactttgaa gtggattata
    30601 ctgactctat agtaaatata aaaaatcaaa atgagaaagt aattttaaat aaatctatca
    30661 aaaaatagta agggggcctt aataggcccc aatttttttc ttttcta
//
LOCUS       DBGJFF010000035        30702 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.035, whole genome shotgun sequence.
ACCESSION   DBGJFF010000035 DBGJFF010000000
VERSION     DBGJFF010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30702
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.035"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(38..889)
                     /locus_tag="ACOIAF_001645"
     CDS             complement(38..889)
                     /locus_tag="ACOIAF_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HGZ2668659.1"
                     /translation="MSCILKLKQIYEDLTEVDKKIADYILNNTEAISKLSVSELASNS
                     KTSTASIVRFSRKMGYSGFGDLKIEIAKDLMGKENEYTYVDENYDCNIDSVINKITNK
                     NIETINQTRLLNEKDIIKEAVEQIIKAKNVYIFGVGGSALVALDLQMKLLRINKQAFT
                     SLDSHTQLMVSSNVDKEDIAIAISYSGESKEVIKSIENAKLKGCKVICITKYSDNHLS
                     KISDLKLVVPNIEKRLREGAISSRIAMLTLIDIIYISIIQENLNEAEQKLQETKRILD
                     YLNINNA"
     gene            complement(906..1826)
                     /locus_tag="ACOIAF_001646"
     CDS             complement(906..1826)
                     /locus_tag="ACOIAF_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HGZ2668660.1"
                     /translation="MYFLGVDGGGTKTTFTLVDEELNIVGTITKGTCHYNQIGFDNLT
                     KLLITGLEEVCKDAKINVEEITYAFVGLAGYGKIKEVLYALEVATKNAYSHINYTLGN
                     DVEIALAGSLNGEKGINIIAGTGSIAQALDKDGNLHRCGGWGYVLGDEGSAYYIGMAT
                     LKMFTMQSDGRCSKTKLYDLIKRHLNIENDYDIIKYVNDEIQGDRIEIAKFATICLKA
                     VIEGDNTASKIFDDAAYELSRLIIGLEHHFEQGTKIKVSYSGGVFKSGDLILEPLKKY
                     LNKTRFGIVRPVLTPDLGACLLAKKKYYSK"
     gene            complement(1936..2850)
                     /gene="murQ"
                     /locus_tag="ACOIAF_001647"
     CDS             complement(1936..2850)
                     /gene="murQ"
                     /locus_tag="ACOIAF_001647"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HGZ2668661.1"
                     /translation="MLIKTLENLVTEGRNKNTLQIDKEDTLGIIELINNEDKTVAYAV
                     EEQKESIAKAVNIIVDRMKQGGRLFYIGAGTSGRIGILDATECPPTYGVDFELVQAII
                     AGGNQAIFKAIEGAEDDKELGKQDIIDRGVTSKDVICGIAASGRTPYVIGAMEYAKEL
                     GCAVLSITMNPNSEMSKKADLPINIIVGAEVIMGSTRMKSGTAQKMVCNMLTTASMVK
                     MGKVYSNLMVDVKTSNEKLVERAKRIIMIATNVKYDVAEKFLEEADNSVKLAIFMIKS
                     GLDKDSAKSILDRQEGYISEALKSIEKL"
     gene            complement(2949..4046)
                     /locus_tag="ACOIAF_001648"
     CDS             complement(2949..4046)
                     /locus_tag="ACOIAF_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HGZ2668662.1"
                     /translation="MGRLGISIYPEKTTSKEIYDYIDKAAENGFTRIFSCLLSVKDTK
                     ENIIKKFKSINEYAKFKGFEVILDVNPRVFGELEISYKDLTFFKKTKADGIRLDIGFT
                     GSEEALMTFNPQNLKIEINMSNNTNYIDTIMDYQPNKENLTGCHNFYPHRYSGLNLEH
                     FIKCTERFNKYGLRTAVFVTSQNETTFGPWPVTDGLPTIEMHRNLPIDIQVKHIIAMN
                     NINDIIISNCYPTYEELKVLGSMRKDMVTFDVAIEENIREVEKKILFEEIHFNRGDIS
                     DSLIRSTQSRVKYKGYKFELFNTPKIIKRGDIVIESSEYGHYAGELQIALTDMKNSGK
                     SNVVGYIRKEELFILDYIKPWQKFNFKPSTK"
     gene            complement(4279..5625)
                     /locus_tag="ACOIAF_001649"
     CDS             complement(4279..5625)
                     /locus_tag="ACOIAF_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HGZ2668663.1"
                     /translation="MLDKFTEFLDTKLSTSMARLAEQRHLRAVRDGIVASLPLIIVGS
                     FFLILAFPPLPDSWGVTQYLKENAVTILLPYRMTFFIISVYSTFGIGYSLAKSYKLDG
                     LTGGLLATATFLLTIVPTAIEGLTPDLLKIVEKSPELLAWYNSIPQGWQMPMANLGGG
                     SMFVAIVTSIIGVEIYRFTDKSKFKITMPEAVPASVTRSFEALTPAALIIILMAIVTY
                     FLGFDWHGFIAKLVTPLVSATDTLPSVIILIFLITFFWSFGIHGVSIIGSLARPLWIQ
                     LLDANTASYAAGQAVPGIAAEPFFQWFIWIGGSGATIGLALLLAFRAKSAYGKSLGKA
                     TVIPAIFNINEPIIFGAPIVLNPILIIPFVVAPIVMATVSWFAMSANLVSRVVVTAPW
                     TLPGPVGAYLATGGDWRASVLNLLCIAISIIIYYPFVKMYDKRLLHEEKVEEQEAY"
     gene            complement(5714..6022)
                     /locus_tag="ACOIAF_001650"
     CDS             complement(5714..6022)
                     /locus_tag="ACOIAF_001650"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGZ2668664.1"
                     /translation="MRVLFVCSSGMSSAIAVNALQKEGAKNGIDIDVLAVGTQEFENE
                     VKNGWDIAMVAPQVRHRFDYLKAFADEVSVPCALIQAQAYSPLGGPKLLKQVQELLSK
                     "
     gene            complement(6284..6682)
                     /locus_tag="ACOIAF_001651"
     CDS             complement(6284..6682)
                     /locus_tag="ACOIAF_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668665.1"
                     /translation="MSNNKKKKRKKNNNYKNAKSTKSNKNTKKQMEKNQRTDYNIPTI
                     SFKSGLILIVSSLVSTVVFPLLLSIFGIDNRFGVVIGNSLITSFAVAYTRYFVETKRG
                     FSLGFFRLYILFALSFAIIGYFWIYKGIHI"
     gene            complement(6684..7835)
                     /gene="anmK"
                     /locus_tag="ACOIAF_001652"
     CDS             complement(6684..7835)
                     /gene="anmK"
                     /locus_tag="ACOIAF_001652"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase AnmK"
                     /protein_id="HGZ2668666.1"
                     /translation="MYSVGLMSGTSLDGVDAVLAEISENGRNTKVKQIEFITLEIPKD
                     IKDEIRKCCIEEESSVDLICSLNFKLGYLFSKAVKSVCHKANFHIANLDFIASHGQTI
                     FHIPRSYNNFVSSTLQIGEPAVIAYETNTKVISNFRVMDIAAGGEGAPLVPYSEFLLY
                     SDNNKNLALQNIGGIGNITIIPKSCNIDDVFAFDTGPGNMIIDGVCQRLFNRKYDKNG
                     YFASKGKINEEMLKDLMSHKYLSQAPPKSTGREVFGQVYLDNMLNKYKHVDKYDLIAT
                     VTMFTAKAIYYNYKNFILPKVNVDTLLIGGGGAHNLTLIGYIKELLLEVEVLTQDEYG
                     YSSDAKEALAFVILGNETLNNSFSNVISATGAKNKVILGNITPKPFGGK"
     gene            complement(8275..9294)
                     /locus_tag="ACOIAF_001653"
     CDS             complement(8275..9294)
                     /locus_tag="ACOIAF_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HGZ2668667.1"
                     /translation="MLVKYLKGRSKYIALPLVVVSIINIYLFAIDIFKNKYSELIYLD
                     FLVLFIIMVFFVIDYINFRNSYTNLYNCIENSGEIDSYLVDGQSFEENLIKDIIENLK
                     IKNNSDIETYKQSLKELDEYIAKWVHEIKIPISSLSIITDRLSSIEDSLDIKNQVAKI
                     NFLVNSILYSSRSNTMFEDVFINKFNLEKLVKMSIKNNSFLLIKNNVEVSLNELENDV
                     YTDSKCMSYVLDQIINNAIKYSKEVGKIEFNSKKLENGVVLSIKDFGIGINEEDISRV
                     FDKGFTGKNGRNQLYKSTGMGMYFVKKMIDSLGHEIEVCSKIGSYTIFNIYFYDISDY
                     LSLDS"
     gene            complement(9288..9968)
                     /locus_tag="ACOIAF_001654"
     CDS             complement(9288..9968)
                     /locus_tag="ACOIAF_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGZ2668668.1"
                     /translation="MYRILLVEDDIDLSKEIALALEKWGFKVELIDDFEVVLDEFIDK
                     KPDVVLLDVNLPLYNGFYWCEKIRAISNVPLIFLSSRDSDMDLIMGINNGADDYITKP
                     FSIEILVTKINGIIRRVYNYSDSNSILYCEDLMFDVGKGIIKHKYKDKSIELTKNEIK
                     ILTLLLKNKNRVVSRESLMMTLWDNDEFVTDNALTVNMNRLRSKVKELGFDDFIKTKK
                     GIGYIIQC"
     gene            complement(10120..12027)
                     /locus_tag="ACOIAF_001655"
     CDS             complement(10120..12027)
                     /locus_tag="ACOIAF_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HGZ2668669.1"
                     /translation="MSILGIAYNNFKSNIRTYLAFFISMVFSVVVLTNFELLRYGDAI
                     NVLQDENKKFTLSVLVAVIMILSVFLFFFIWYATNIFFKNRSKEIGILSFMGLDLYTI
                     GKIYFVENMLIGISSCITGIIIGIITSRFFQVVIIKLSGFNIKVSNGLSIEAILYATL
                     IFISIFIIMAVKGFITICRSSVINLINISKKQEKIPKVGIGIYILAIVSIIVIGYGYY
                     LSTNIRTGDITSVLPVIGIIIVGTYGLFKSVMPVIFDIIIKNKKILFNGNNIIAINNI
                     NYRLNKNYKTYAIIAIIITTTISTLGASVAMKHIHKNAQEQRNVYTISIVSMDKDEVN
                     KKQIENIIKDTNKIKYSVNPELTILKKTDIKEAYDTTDTIMKYSDFCNTLKVNGNDKE
                     LKEYGERLVSGNNVIELKSPQTIASIATKEAKIKLDGQLYNISRGEVKVPVLGTGLNQ
                     SIIVVNDDTYFKLRDKGQQVYFYGAKVFQEENSQVMFNNIEKNLSLKNGYIQNGYKAQ
                     GESQWMKFAYVVLVFLFLVFAIVAGSIIYMKIYSDAYEDKEKYKILLKIGTTEKDINR
                     AILKEVAIFYTLPMLSATISSYFALRLAGDLLMTDLFGIYILSLVICLIIFIIYGAVS
                     VNKFKSVVYKN"
     gene            complement(12028..12780)
                     /locus_tag="ACOIAF_001656"
     CDS             complement(12028..12780)
                     /locus_tag="ACOIAF_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2668670.1"
                     /translation="MKEILKIKNISKDYGIKGFKTNVLKNISLTVNEGDFISIMGPSG
                     AGKTTLLNLMSTLDKQTSGEIILDGINISKVKNNELSKLRREKIGFIFQDYNLLDNMK
                     LMDNIALPLALGKKKSKEIEAKVFSIAKKFGLENHLDKYPYQLSGGQKQRGAAARSLI
                     TNPTVIFADEPTGALDSKSAYELLESLEKINRENNATIIMITHDTLTASYSNEVYMIN
                     DGNIKCKLNKGNSRKEFYGKIMDMLASMGGEM"
     gene            complement(13096..14250)
                     /locus_tag="ACOIAF_001657"
     CDS             complement(13096..14250)
                     /locus_tag="ACOIAF_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGZ2668671.1"
                     /translation="MINKAIKFKFIIFYFFSYIIFAFASTKFTPFLSKLGYSAFERGI
                     ILSSYAITNILFQLLFGVLADKYQTMKKIVLISLSVYGIASVIMFSTESATFIIYLLL
                     VALSGGLLNTCCSLYDTWIIGCGDNINNYLSFIKTFGSIGWAIGSMVASYLILRFSYF
                     GLSVAIGLIVVLLIINILLLPDIEKINKKINVTNKDIIELLKDKKYILLVCILFLLYS
                     MVVANNCTVIDKMIALNATNSQISLKWSLQSVLEIPTYLAGSYLLKKYSSLNLLKLSA
                     IMITVQFLLFALTNDSNIIIILCVFQVFSTPLILITSKRLIFEVSPKKLRSSSQLIAL
                     SIFMGGSSLIIPTVAGFLSINIGYNYTLMVLSLFGCLAYCLIVLLNYLQE"
     gene            complement(14253..14720)
                     /locus_tag="ACOIAF_001658"
     CDS             complement(14253..14720)
                     /locus_tag="ACOIAF_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HGZ2668672.1"
                     /translation="MFFKRKKEAITINSYIDGKIINLGEIPDKVFSTKVMGDGFAMIA
                     KDNILKSPTKGVISFIAQTNHAIGIKLQNDVQILIHIGLDSATTKKENFKLEVKQGQE
                     VNMGEKLVEIDNKLLENTLYIPLVIVENPNTVKYHLSNLNQEGNSGIKVINFD"
     gene            complement(14734..15222)
                     /locus_tag="ACOIAF_001659"
     CDS             complement(14734..15222)
                     /locus_tag="ACOIAF_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668673.1"
                     /translation="MITNKIKNITAVLKDENIFLSVVMVCFLILPVLYIVYLELTSLF
                     TNISFSQLLNNDVNASLNLISNFSAVYSAYVIYNIKKNKEIKENMIAFYLVLLGQIFQ
                     IYTITIVIMFTYIYKFVGIKQIISSIKELQWGKSYKALFSSIAVCLISLFVMIIRIKL
                     IM"
     gene            complement(15236..16567)
                     /locus_tag="ACOIAF_001660"
     CDS             complement(15236..16567)
                     /locus_tag="ACOIAF_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HGZ2668674.1"
                     /translation="MERKRQRILIAGGGSTYTAGIVTMLIESVAKFPIESIKLYDNND
                     ERQRKVAEACAIIVREKNPEIKFSYTTNPEEAFTDIDFVMAQIRVGLYALREQDEKIP
                     LKYGVVGQETCGAGGIAYGLRTIGPIIEMIDYMEKYSPNAWMLNYSNPAAIVAEACRV
                     LRPNSRIINICDMPVCLEEIFARVLGLNSRKDFDVRYYGLNHFGWWTSIKDKEGNDLM
                     PKVQEYCAKKGYEEFTPQGQHKESSWLETMRAAKDLLEIEPTTLPNTYLKYYLMADET
                     VEHANPNYTRANEIMDRREKDTFEECERIIKNGTARDTWFDASEHSQFIVELACALAF
                     NTQERFLLIVPNNGAIENFADDAMVEIPCLVGKDMVEPMSIGKIPTFQKGLMEQQVAS
                     EKLAVEAWIEKSYQKLWQSFTMSKTVPSAKVAKEILDEMIVANKDFWPTFK"
     gene            complement(16581..18146)
                     /locus_tag="ACOIAF_001661"
     CDS             complement(16581..18146)
                     /locus_tag="ACOIAF_001661"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucoside-specific PTS transporter subunit
                     IIBC"
                     /protein_id="HGZ2668675.1"
                     /translation="MMKKIQRFGGAMFTPTLLFAFAGIMVGFSIVFQNQSIMGSLATP
                     ENIWYQFWGVISSGAWMVFNQLPLLFAISLPIALAKKQQARACMEALATYLTFNYFVG
                     SMLSFWGKSFGVDFAAEISAGSGLVSIAGIKTLDTGMVGALLISGIVIYIHNRFYDKE
                     LPDFIGLFRGSSLVVAICFFVMIPVALLTCFIWPHIQNVIRHLQTFFINSGNIGVWCY
                     AFLQKILIPTGLHHFVYAPICYDSVVVPGGTSVYWATHIQDFQTSAKTLKEMYPIGFS
                     LSGLSKVFGSLGVFGAFYVTAKPEKKKKVLGLMIPATLTAVLTGITEPLEFTFLFVAP
                     LLFLVHAFLDACLQTISFALGVVGDFGGGIINWVVLNWLPLGMYHWKVYVVQVVVGII
                     FSFIWFFVFTFLIKKFDMKTPGREEDSEETKLYTKNEYLETKDEKGNKLSKASQQASE
                     YIKLVGGAENVVDVTNCATRLRLTLKDDSIISKEEDFKAVGAHGLVHNGKAIQIIIGL
                     SVPSVREEFENLL"
     gene            complement(18315..19100)
                     /locus_tag="ACOIAF_001662"
     CDS             complement(18315..19100)
                     /locus_tag="ACOIAF_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HGZ2668676.1"
                     /translation="MRLEEMIFFNYNKLNDTDLLIWKYITANKKHCSKMSINELAMKC
                     NVSRATITRFVKKLDLKGFSEFKVLLSWESEKVHVIENQSFDIACNSIIKYVEDQKNK
                     DYDALCKLLYESRTIYAYGSGDIQNVVAKQIKRMFLSCQEIIYDFEGRTFDDSFYNIV
                     SKNDVMIIISLSGENENTLNIARRLKLIGTKIISITEFKNNTLTDLSDESIYISATNI
                     SFLQMHPSYKLTMLYFILIELLFIKYSIYKRHRMITEGIDCKL"
     gene            complement(19267..20466)
                     /locus_tag="ACOIAF_001663"
     CDS             complement(19267..20466)
                     /locus_tag="ACOIAF_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HGZ2668677.1"
                     /translation="MKKKVLFLVIILAFIILLGNSTYSQKNQLSEEEKVDDFNYVYNV
                     VKTGYPYLGVNKRLNNIDWLANKDEYTKRIKNTENDEEFIEELSSILSDLNNKHTEVI
                     DNKKRYELFKKSYSNNDWYDFLNDKKVVDRYDSMNLKIKMPDDIFVKKELILKDVIKG
                     QIGYMYLPSMSSKNGSINNDLKIIGDYINTLEKHKALIIDIRGNLGGSDRYWQGIVSK
                     LIKNDVKINGYRIYRNNNEIVKKYTNARNIKLNPVENLPASVKENAPKEIIKDFNDFE
                     DTSYTIKSTDDSKFKGNIYLLVDRKIYSSSESFAMFCKENKFATLVGETTGGDGGCID
                     PVLFNLKNSGLIVRMASCMYLNKNGICDEEFKTIPEFKVKDCKRTADFKDDNCIRKAL
                     ELENINY"
     gene            complement(20988..22325)
                     /gene="xylA"
                     /locus_tag="ACOIAF_001664"
     CDS             complement(20988..22325)
                     /gene="xylA"
                     /locus_tag="ACOIAF_001664"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HGZ2668678.1"
                     /translation="MSEIFKGIGQIKFEGVKSDNELAFRYYNPEQVVGNKTMKEHLRF
                     AMSYWHTLCGEGNDPFGVGTVERPWNNITDPIEIAKIKVDAGFEFMSKMGIEYFCFHD
                     RDIAPEGRDLEETNKILDEIVEYIKVNMEKTGIKLLWGTANMFGNPRFVHGASTTCNA
                     DVYAYAAAQVKKAMEITKYLGGENFVFWGGREGYETLLNTNTELEMDNFARFLQMAVD
                     YAKEIGFTGQFLIEPKPKEPTKHQYDFDTATVLGFLRKYNLDKYFKMNIEANHATLAG
                     HTFQHELNIARINNVLGSIDANQGDLLLGWDTDQFPTNIYDATLAMYEVLKQGGIAPG
                     GFNFDSKVRRASFEVEDLFLAYIAGMDTFAKGLLIAHKLLEDEVFENFTKERYASFSE
                     GIGKDIVEGKVGFKELESYALQMPVIKNKSGRQEMLEAILNRYIYEVDTISNK"
     gene            complement(22421..23971)
                     /gene="xylB"
                     /locus_tag="ACOIAF_001665"
     CDS             complement(22421..23971)
                     /gene="xylB"
                     /locus_tag="ACOIAF_001665"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HGZ2668679.1"
                     /translation="MKLEYVLGVDIGTSGTKTVLFDKLGNTIKSCTYEYPLIQEKSGW
                     AEQDANDWWKAVVESIREVVQSSNISSECIKGIGLSGQMHGLVMLDNEGKTLRNSIIW
                     CDQRTVKECEEITDLVGEERLIEITANPALTGFTASKILWVRNNEPDIYKNTNKILLP
                     KDYIRYKLTGEYATEVSDASGMQLLDIRKRDWSDEVLEKLNIDKNLLGKVYESQEITG
                     YVTRDVASLTGLKEGTIVVGGAGDQAAGAIGNGIVKDGVVSSTIGTSGVVFAYTKEPK
                     IDKEGRVHTFCHAIPNTWHVMGVTQGAGLSLKWFKDNFCQSEVEVSNSLGEDVYEIIN
                     NQVSQVPTGCNGLLYLPYMMGERTPHLDPYARGVFFGLSPIHSKKEIARSIMEGVSYS
                     LKDCMDIIENLNIDVNEVRASGGGGKSKVWRQMQADMFNQDVYTINSSEGPALGVAIL
                     ALVGAGIYENIQKACDAIIKTSTKLEPISENVDTYNIYHRLYKRIYKSLKDDFKLLDE
                     VVNAKINN"
     gene            24179..25339
                     /locus_tag="ACOIAF_001666"
     CDS             24179..25339
                     /locus_tag="ACOIAF_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HGZ2668680.1"
                     /translation="MVTDKYTIREMNERLVLEQIIKNGPISRASIASTIGLNKATISA
                     ITKKLIDESLVHEIGIGNSTHSGGRKPILLVFNKCAGISLSMDIGYDYIFSSLSYLDG
                     TIINSKKLTDIQVSKDNVIQLIDEIINSYNISKIDTPYKVIGLTLAIHGITCENKVLF
                     TPYYNLNEIDLYSILSKKYDFPIHIENEANLTALAENTFSTVHNSLLSLSIHSGFGSG
                     IIINNKLYSGRNGMSGEIGHTIIMPNGKLCPCGNRGCLEQYCSEKKVFEQLSSLENIP
                     KIDSDIVKQLYYEDNQNAKKVIHEFCSYLTIAINNAITTYAPEIIYLNSQIISDIPEI
                     LQITKDMLVSSFNKGINIEISSLGSEASLYGGSAVNIKSFLNIQNLTLINEI"
     gene            complement(25495..25899)
                     /locus_tag="ACOIAF_001667"
     CDS             complement(25495..25899)
                     /locus_tag="ACOIAF_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGZ2668681.1"
                     /translation="MNSIILMSHGNMAEEVLNSAKMIIGDSIDYPTVNMKQDDGIEGT
                     IEKLKKALEVYKESSEIIIMADLLGGTPCNAALLVANNDSRIKIISGLNLGMVIESAF
                     LDTKELAAKLEKIGKDNISMVEQNASLLSDDE"
     gene            complement(25920..26402)
                     /locus_tag="ACOIAF_001668"
     CDS             complement(25920..26402)
                     /locus_tag="ACOIAF_001668"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGZ2668682.1"
                     /translation="MEMKGIKNIRIDDRLIHGQVATMWSNKLGVTRLMVVNDAVANNS
                     VQKQVLRMATPAGIASSIITEETAIKNITAGKYEGQNVLLIVKSPVDLIPFVEAGFKI
                     HNINVGNMSSRKDTTVLRPNISVTEEEKEAFKRLLDEGIEITTIMTPDDKKTYLVDIL
                     "
     gene            complement(26422..27231)
                     /locus_tag="ACOIAF_001669"
     CDS             complement(26422..27231)
                     /locus_tag="ACOIAF_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HGZ2668683.1"
                     /translation="MSDKVSLNKSNEKVKKSVLWRWFFTSSVSSNYEKMQALAYCYAV
                     LPFLKVVYKDNPEKLQSAVLNHLQFFNTNPWVAPYILGINVAMEELSSEETEEAVASI
                     KTGLMGPVAGLGDSLFVVIPWTIFGAIAANMAIDGSPFGILLWIVVSVALKLLSIPLF
                     NAGYTSGTKLISSIEKSLKILTESTSILGLMVVGALIPSVVKANVALNFQQGDFTMKG
                     QEILDQIMPGLVPAILVVIVYWALKKNIKPIYLILGVMVVSIILYALGILK"
     gene            complement(27224..28003)
                     /locus_tag="ACOIAF_001670"
     CDS             complement(27224..28003)
                     /locus_tag="ACOIAF_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HGZ2668684.1"
                     /translation="MEPQLWQLLLIVLYGFFINYEKNSTMFGTYQPVTAGFITGLILG
                     DINTGLYIGGTLQLLSLGISNFGGASIPDYQTASIVATFITITTKQEASVGISIGIPV
                     ALLMVQLDVLRNTIGIWLVHKAEDGAKKGNYKNITYMQMLGVLLTAATTGIPVALSVI
                     FGPSLINTILKYTPEWLTGGLTVAGGLLPAVGIGLLLRYLPAKEYFSYLVIGFVLAVY
                     MKVPLLGVALIGGAIALIIYKKNLENQEQQYTVVGGMDEDE"
     gene            complement(28003..30081)
                     /locus_tag="ACOIAF_001671"
     CDS             complement(28003..30081)
                     /locus_tag="ACOIAF_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HGZ2668685.1"
                     /translation="MIKISEDYIEKRFDNELLRIEAWGKNSLRIRSFVDQNFVDENYA
                     LNEKPKLNKDDITINKNEDGSAIIKNGKIKAVLDHRDRITFYNDKNEILLKEYIRLRA
                     VKHDDGGEDVGTIEITKDFNSTLKLKSREYRPSYSGEFEVTTRFESEPSEKIFGMGQY
                     QHKFLDLKNTVLELAQRNSQISVPFYISSLGYGLLWNNPGIGKVSFAKNMTEWKMFST
                     NFIDYWITCGESPKELNKNYSQVTGTVPMMPENLLGLWQSKLRYRTSEEVLDVVKEYS
                     KRGIKLSSIAIDYFHWPKQGEYKFDLDYWKNPKELVKKLKEEYSVEPIVSVWPTVQSD
                     AENYNDYLENGYLVNVNRGVRMTMQIQGNTVFVDMTNENAREYVWDRIDKNYKQLGID
                     YYWLDVAEPGYSVYDFDNYRYKKGNVLSCGNIYPIDYLKMIYDGLHNDIESVVTLVRG
                     AWAGAQKYGALVWSGDIDSSFEAFNNQVNTGLNMGLAGIPWWTTDIGGFHGGNPKDPE
                     FRELMVRWFQYATFSPILRMHGDRLPHSKPLSNKGGGSMVTGAPNEIWSYGEEVEVIL
                     TKFIKIRESLKTYLKKLMKEAHEDGTPVMRTLFYEFPEDDKTWEVDNTYMLGDEILVA
                     PIMNYKDRSRKVYLPKGHTWENIFSGVSYEGGKTYEVECPLEEIPIFLKQDSSYNFKE
                     LKNILGEVKS"
     gene            30391..>30702
                     /locus_tag="ACOIAF_001672"
     CDS             30391..>30702
                     /locus_tag="ACOIAF_001672"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HGZ2668686.1"
                     /translation="MNTLKHESVNIHDDIGVKLYLSIDNGSFTVKHWHYSLEVILILN
                     GSFNLFIDNERLNLKENDLIILNPKQIHSAICSDGNESIVLQIPYKFLEENIPNINEL
                     RI"
BASE COUNT        10097 a         5445 c         3205 g        11955 t
ORIGIN      
        1 gcctaactta tttgtttagt tgatatataa cctagtttta agcattatta atatttaaat
       61 aatcaagtat cctttttgtt tcttgtagtt tctgttctgc ctcatttaag ttttcttgaa
      121 ttatagaaat atatattata tctatcaacg taagcattgc tattcttgaa gaaatagctc
      181 cttctctaag tcttttttct atatttggta ctactagctt taaatcacta atcttactaa
      241 gatgattgtc actatattta gttatacaaa tgaccttaca tccttttaat tttgcatttt
      301 ctatagactt tattacttct tttgattctc cagagtaaga tattgctata gctatatctt
      361 ctttatctac atttgaagat accataagtt gtgtatgact atctaaagag gtaaatgcct
      421 gtttatttat ccttaaaagt ttcatctgta aatctagtgc aactaaagcc gaaccaccta
      481 ctccaaatat atatacattt ttagccttta ttatctgttc aactgcttct tttattatat
      541 ctttttcatt caacaacctt gtttggttga tagtctctat gtttttgttt gtaattttgt
      601 ttataacact atctatatta caatcatagt tttcatctac atatgtgtat tcattttctt
      661 tgcccatcaa atctttagct atctcaattt ttaaatcccc aaaacccgaa tatcccattt
      721 ttctagaaaa cctaactata cttgctgtac ttgtttttga gtttgacgcc aattctgata
      781 cagaaagctt acttatagcc tctgtattat taagtatgta atcagctatt tttttatcta
      841 cttccgttaa atcttcatat atctgtttta attttaaaat gcaactcata ctctatcacc
      901 tctacctatt tactataata tttcttctta gcaagtaaac atgctcctaa atctggagtc
      961 aacactggtc tcactatacc aaatctcgtt ttattcaaat attttttaag tggctctaat
     1021 attaaatctc ctgacttaaa cactccacct gaataagata cctttatctt tgtaccttgt
     1081 tcaaaatgat gctctaatcc tattataagc ctacttaatt cataagctgc atcatcaaat
     1141 attttactag cagtattatc accttcaata actgctttta aacatatcgt agcaaacttt
     1201 gctatttcta ttctatctcc ttgtatttca tcatttacat attttattat gtcataatca
     1261 ttctctatat taagatgtct ttttattaaa tcatataatt ttgtttttga acaccttcca
     1321 tcagactgca ttgtgaacat ttttaaggta gccattccta tataatacgc tgaaccttca
     1381 tctcctaata catagcccca tcctccacat ctgtgaagat tcccatcttt atctaaagcc
     1441 tgtgctattg aaccagtacc tgcaattata tttatacctt tttccccatt taaagaacct
     1501 gctaatgcta tttctacatc attacctaga gtataattta tatgactata tgcatttttt
     1561 gttgctactt ctaatgcata caatacttct tttatttttc catatccagc aagtccaaca
     1621 aatgcatacg ttatttcttc tacatttatc ttagcatctt tacaaacttc ttccaaccca
     1681 gttattaata atttcgttag attatcaaat cctatttgat tataatgaca agttcctttg
     1741 gttatggttc ctactatatt taattcttca tctactaatg taaatgttgt tttcgttcct
     1801 ccaccatcta cacctaaaaa atacatacta tcctcctaca atatctttaa taattctttc
     1861 aatacttaat atctaaaaat aaccttccaa tgctgttttt ttaatattga aaggttatca
     1921 gtactattat ttattttaca atttttctat tgattttaaa gcttcactta tataaccttc
     1981 ttgtctatct aggatactct ttgcactatc cttatctaaa ccacttttta tcataaatat
     2041 agccaattta acgctattat ctgcttcttc taagaatttt tctgcaacat catattttac
     2101 atttgtagct atcattatta ttctttttgc tctttcaacc aacttttcat ttgatgtctt
     2161 aacatctacc atcaagtttg agtatacttt tcccatctta accatagaag ctgttgttaa
     2221 catattacat accatttttt gagctgtacc acttttcatt ctagttgaac ccattattac
     2281 ttctgcccct actataatgt ttattggtaa atccgctttt ttactcattt cactattcgg
     2341 attcatagtt attgatagta ctgcacaccc caattcctta gcatactcca ttgcacctat
     2401 aacataagga gtccttcctg atgcagctat accacatata acatccttag atgtaactcc
     2461 tctatctatt atatcttgct ttccaagttc tttatcatct tcagcacctt ctattgcttt
     2521 aaatattgct tgatttccac cagctattat agcttgaaca agctcaaaat ccactccgta
     2581 agttggagga cattctgtgg catctagtat tcctatcctc ccactggtac ctgctcctat
     2641 atagaataat ctacctccct gtttcattct atctactatt atattaacag cttttgctat
     2701 actctccttt tgttcttcaa cagcataggc tacagtttta tcttcgttat ttattaattc
     2761 tattatgcct aatgtatctt ctttatctat ttgcaatgta ttcttatttc taccttctgt
     2821 aactaaattt tctaaagttt ttattaacat atcatttcct cattttctta atattaaatt
     2881 tttaaacaca ttttaattcc aactttcata attatatttt tataataaat taatattttc
     2941 ataataaatt attttgttga gggtttaaaa ttaaactttt gccatggttt aatataatct
     3001 aatataaata attcttcttt tcttatatat cctactacat tcgatttacc actgtttttc
     3061 atatcagtca aggctatttg taactcccct gcataatgac catattcact actttcaatt
     3121 actatatctc ctctttttat tattttagga gtattaaata gttcaaattt gtatccttta
     3181 tatttaacac gagattgagt agaccttatc agactgtcag atatgtcccc tctattaaaa
     3241 tgtatttctt caaagagtat cttcttctca acttctcgta tattttcctc tattgctaca
     3301 tcaaatgtaa ccatatcttt tctcatagaa cctaatacct ttagctcctc atatgttgga
     3361 taacaatttg atattattat atcatttata ttattcatag ctattatatg ctttacttga
     3421 atatctatag gtagatttct gtgcatctca attgttggaa gcccatcagt tacaggccat
     3481 ggtccaaatg tagtttcatt ttgacttgtt acaaaaactg ctgttcttaa tccatactta
     3541 ttaaatcttt ctgtacattt tataaagtgt tctaaattta atccactata tctatgtgga
     3601 taaaagttat gacatcctgt caagttttct ttatttggtt ggtagtccat tattgtatct
     3661 atataattag tattattact catattgatt tctattttaa gattttgagg attaaaagtc
     3721 attaatgcct cttcagaacc tgtaaatcct atatcaagcc ttattccgtc tgccttcgtc
     3781 tttttaaaaa aagtcaaatc tttgtagctt atttctaatt caccaaatac tctgggattt
     3841 acatcaagta ttacctcaaa acctttaaac ttagcgtatt catttatact tttaaatttt
     3901 ttaattatat tttctttagt atctttaact gataataaac atgaaaatat acgtgtaaat
     3961 ccattttcag cagctttatc tatataatca tatatttcct tagatgttgt tttttctgga
     4021 taaattgata ttcctaatct tcccatctgt gtcatctcct tatatcctat atgtgtttaa
     4081 atttatttcg aattacacga aactttttta aaaatttggt gaaaaaaagt ttcatatata
     4141 tttataaaaa aagaaaagtt taccttttct ttttttataa acaatcaaca aaatatcttc
     4201 gttacttatt tcataactgt tatactttga aatttatact aatctaatat agtttttaat
     4261 ttccaatata gtttctaatt aataagcttc ttgctcttca actttttctt catgaagcaa
     4321 tctcttatcg tacatcttga caaatggata gtaaattatt attgatatag ctatacataa
     4381 taagttaaga actgaagctc tccaatctcc accagtggct aaatatgccc ctactggtcc
     4441 tggtaatgtc caaggagcag taactactac tctggatact aaattagcac tcatagcaaa
     4501 ccatgaaact gttgccatta ctattggtgc tacaacaaat ggtattatta atattgggtt
     4561 taatactatt ggcgctccaa atattattgg ttcatttata ttgaatattg ctggtattac
     4621 agttgctttt cctaaagact ttccataagc tgattttgct ctaaatgcaa gaagtaaagc
     4681 taatcctatt gtagctccag aacctccaat ccatataaac cattgaaaaa agggttcagc
     4741 agcaatacct ggaactgctt gacccgcagc atatgaagct gtattagcat ctaataattg
     4801 tatccataaa ggtcttgcca atgaacctat tattgataca ccatgtattc caaatgacca
     4861 aaaaaacgtt attaaaaata ttagtataat tactgatggt aatgtgtcag tggctgaaac
     4921 taatggagtt actaatttag ctatgaagcc atgccaatca aaccctagga aataagtaac
     4981 aatagccatt aaaattatta ttaatgcggc tggtgtcaga gcttcaaaag accttgtgac
     5041 tgatgctgga actgcttctg gcatggttat cttgaattta gatttgtcag taaatctata
     5101 aatctcaaca cctattatag atgttacaat agcaacaaac attgaacctc caccaagatt
     5161 agccattggc atttgccatc cttgaggaat tgaattatac caagctaaaa gttctggact
     5221 cttttcaact atctttaata aatcaggggt aagaccctct attgcagtag gaactattgt
     5281 caaaaggaat gtagctgtag ctaataaacc tcctgtcaat ccatctaact tatatgactt
     5341 tgccaaacta taacctatgc caaatgtaga atatactgat ataataaaaa atgtcattct
     5401 atatggtagt aatattgtaa ctgcattttc ctttagatac tgagttaccc cccatgaatc
     5461 tggtaatgga ggaaatgcta atatcaagaa aaatgaccca actattataa gtggtaatga
     5521 agctacgatt ccatctctta cagctcttaa gtgacgttgt tctgccaatc tggccataga
     5581 agttgataat ttagtgtcta aaaattctgt aaatttatct agcatctttt ctctccttaa
     5641 ttaatattta tttttattat cattaaaatt tttataaaat caactttctt ctaatctaac
     5701 aacactgcaa ctactattta ctcaacagtt cttgaacttg ttttaataac tttggtcctc
     5761 ctaatggact ataagcttgt gcttgtatta atgcacatgg tacactaacc tcatcagcaa
     5821 aagcttttaa atagtcaaat ctatgtctga cttgtggagc taccatagca atatcccatc
     5881 catttttaac ttcattctca aactcttgag taccaacagc caaaacatct atatctattc
     5941 cattttttgc tccttctttt tgtaatgcat taactgctat agcacttgac atccctgatg
     6001 aacaaacaaa taatactctc atattgattc cccctgtttt tttatttctt ttcaatcatt
     6061 atagcatgta tttttgatta caggaatacg tttgtatata ttttttcata ttttacattt
     6121 taaattgtaa ttttatttca tgcatttata tcttgcttta attgctttat ttatctaatc
     6181 attcttttgt ctaaaattac acactactta aaatactcat atcttttctt aaaaaagaaa
     6241 aaagactatc ttattattaa cataaaatag tctcaatact tttttaaata tgtattcctt
     6301 tatatatcca gaagtaacct attattgcaa atgataaagc aaataaaata tacagtctaa
     6361 aaaatcccaa agaaaaacct ctcttagttt caacaaagta tcttgtatat gcaactgcaa
     6421 aagatgtaat taatgaattt cctataacaa ctccaaatct attatctatt ccaaatatag
     6481 ataataacaa aggaaaaact acagtactta caagagatga aactattagt attaatccac
     6541 ttttaaaact tatagttgga atattataat ctgttctttg atttttctcc atttgtttct
     6601 tagtattttt attacttttt gtactttttg catttttata attattattt ttctttcttt
     6661 tctttttttt attattagac atgtcattta cctccaaaag gtttaggtgt tatattccct
     6721 aaaataactt tatttttagc ccctgttgca gatattacat tagaaaaaga attatttaat
     6781 gtttcattgc ccaagattac aaatgccaaa gcctcctttg catcagaaga atacccatat
     6841 tcatcttgag ttaatacttc aacctctaat aataactctt ttatatatcc tattaaagtt
     6901 agattatgag ctcctccacc tccaattaat aaagtatcta cattaacctt tggaagtatg
     6961 aagtttttat aattataata tatagctttt gccgtaaaca tagttactgt tgctatcaaa
     7021 tcatatttat ctacatgttt atatttgtta agcatgttat ccaaataaac ttgtccaaat
     7081 acctctcttc cagtgctttt aggtggcgct tgactaagat acttatgact catcaaatct
     7141 tttaacattt cttcattaat tttacctttt gatgcaaagt atccattttt atcatacttt
     7201 ctattaaata atctttgaca tactccatct attatcatat ttcctggtcc tgtatcaaat
     7261 gcaaatacat catctatgtt acatgattta ggtattatag taatatttcc tattccacca
     7321 atattttgaa gtgctaaatt tttattgtta tcactatata ataaaaattc actatatggt
     7381 actaatggag caccttctcc acctgctgct atatccatta ctctaaaatt agatattacc
     7441 tttgtatttg tttcatatgc tataactgct ggttcaccta tttgaagcgt tgaagataca
     7501 aaattattat aacttcttgg tatatgaaag attgtttgac catgagatgc gataaaatcc
     7561 aaatttgcta tatgaaagtt tgctttatga cacactgatt ttacagcttt actaaataaa
     7621 tatccaagct taaaatttaa gctacagatt aaatcaacac tcgattcctc ttctatacag
     7681 cactttctta tttcgtcttt tatatcttta ggaatttcta gagttataaa ttctatttgc
     7741 ttaactttag tatttctacc attctcacta atttcagcta gtactgcatc aactccatct
     7801 aaggaagttc ctgacattaa acctacacta tacatagtta tcatcctttt aagtttatag
     7861 ttattcctcc ccaaatcaag gtattaagtt taataatttt agaattaatc ttatttcatc
     7921 tttaattacg ataatttact cttacgaaat taatcgagtt aatcttatac acgtttttta
     7981 ccttagctaa atcttacatt aatttatttt cagtgtcaat gtaataccat gtaatttttt
     8041 ttcataaaca aaacatattc tctctccaaa aaccatatta aaatagttac tacaaataaa
     8101 aaacatacca ggtacctatc tgatatgttt ttcatatata ataattatta ttttataata
     8161 tagatagagc atcatttaag gcactaatta aatcttcact attttcaaga actaaattta
     8221 ttcttattgt atatgtcgat ttgtatatga tgatagattt tatagtctaa taacctaact
     8281 atctaggctt aaataatccg atatatcata aaagtagata ttaaaaattg tatatgaacc
     8341 aattttcgaa catacctcaa tctcatgccc taaactatct atcattttct ttacaaaata
     8401 catccccatt ccagtagact tatacaattg atttcttcca tttttccccg taaatccctt
     8461 gtcaaaaacc ctagatatat cttcctcatt tatacctata ccaaaatctt ttatagataa
     8521 taccactcca ttttctaact tttttgaatt aaattcaatc ttaccaactt cctttgaata
     8581 ctttatagca ttattaataa tttggtctaa aacatatgac atgcatttag aatctgtgta
     8641 aacatcattt tctaattcat tcaaactaac ttctacatta ttttttataa gcaaaaatga
     8701 attatttttt atagacattt ttaccaattt ttctaaatta aatttattta taaatacatc
     8761 ttcaaacata gtgttgcttc tactactata caaaatagag tttactaaaa aatttatctt
     8821 tgcaacctga ttcttaatat ccaaactatc ttctatgcta cttaacctat ctgtaattat
     8881 acttaaagaa gatattggaa tttttatttc atgaacccat ttagctatgt actcatccaa
     8941 ttcttttaat gattgtttat aagtctctat gtcactatta ttttttattt ttaagttttc
     9001 tataatatct tttattagat tttcttcaaa agattgtcca tcaactaaat aactatctat
     9061 ttcaccacta ttttctatac aattatataa atttgtgtaa ctatttctaa aatttatata
     9121 atctataaca aaaaatacca ttattataaa cagcactaaa aaatctaaat atattaactc
     9181 tgaatattta tttttaaaaa tatctatagc aaataagtag atatttatta tactaactac
     9241 aactaaaggt agagcaatat acttagacct acctttcaaa tattttacta gcattgtatt
     9301 atatatccta tccccttctt agtttttata aaatcatcaa aacctagttc tttaacttta
     9361 cttctcaatc tattcatatt tacagtaagt gcattgtctg tcacaaactc atcattatcc
     9421 catagagtca tcataagact ttcccttgat acaaccctat tcttattttt taataataat
     9481 gttaaaattt ttatttcgtt tttagtcaac tctatacttt tatccttata cttatgtttt
     9541 ataatccctt tccccacatc aaacatcaaa tcttcacaat acaaaatact attagaatca
     9601 ctataattat agactcttct tattatacca tttattttgg taaccaatat ttctatagaa
     9661 aatggtttgg taatgtaatc atctgctcca ttatttatgc ccattattaa atccatatca
     9721 gaatctcttg atgataaaaa tattaatggt acatttgaaa tagctcttat tttttcgcac
     9781 cagtaaaatc cattatataa tggtaaattt acatctaaaa gtactacatc tggtttttta
     9841 tctataaatt catctaatac tacctcaaaa tcatcaatta attctacttt aaatccccat
     9901 ttttcaagtg ctaacgcaat ctcttttgat aaatcaatat cgtcctctac taataaaatt
     9961 ctatacattt gtatattctc cattatccta atatttcact atgctatgaa ttattaatat
    10021 cataacatat tttatatgaa tttattaccc aaattattta gttaatcaac aaggagactc
    10081 ttattacaag aatctccttt ctctttatta atatatcaac taatttttat acactacact
    10141 cttaaactta ttaacagata ctgctccata tattatgaat attatcaaac atatcactaa
    10201 acttagtata tatataccaa ataaatcggt catcaataaa tctcctgcta atctcaatgc
    10261 aaaataacta gatatagttg cacttaacat tggcaaagta taaaaaatag ctacttcttt
    10321 aagtatagct ctatttatat ctttttctgt agtacctatt tttaacaaaa tcttatattt
    10381 ctctttatct tcatatgcat cactatatat tttcatatat attatacttc cagctacaat
    10441 tgcaaagact aagaataaaa atacaagcac tacatatgca aacttcatcc attgactctc
    10501 accttgagct ttatatccat tttgaatata cccatttttt aaacttaaat tcttttcaat
    10561 attgttgaac attacttgac tattttcttc ttgaaatacc tttgcaccat aaaaatacac
    10621 ttgttgaccc ttatctctta gtttaaaata tgtatcatca tttaccacta taatactttg
    10681 attaagtcct gtccctagta ctggaacttt tacttctccc cttgaaatat tatataactg
    10741 accatcaagt tttatcttgg cttcttttgt agctattgat gctatcgttt gagggctttt
    10801 taactctatt acattatttc cactaactaa tctttcacca tactctttta attctttatc
    10861 attgccattc actttaagag tattacaaaa atcactatat ttcattatag tatctgttgt
    10921 atcataagct tcttttatat ctgttttctt taaaattgtt agttctggat ttactgagta
    10981 ctttatctta ttagtatctt ttattatatt ctctatttgt tttttattca cttcatcttt
    11041 atccattgaa actatgctaa tagtgtacac atttctttgt tcttgggcat tcttatgtat
    11101 atgcttcata gcaactgaag caccaagagt tgatattgtt gtagttataa ttatagctat
    11161 tatagcataa gttttatagt ttttatttaa tcggtagttt atattgttta ttgcaattat
    11221 attatttcca ttaaataata tttttttatt tttgattata atatcaaata taacaggcat
    11281 aactgatttg aataatccat aagtacctac tattattata cctataacag gtagtacact
    11341 agttatatct ccagttctta tattggtaga aagatagtag ccatatccta ttactataat
    11401 tgatactata gctaatatat atatacctat tcctaccttt ggtatttttt cttgtttttt
    11461 agatatattg attagattta ttacactact tctacatatt gttataaaac cttttacagc
    11521 cattattata aatatagaaa taaagattaa agtagcatat agtatagctt ctatactaag
    11581 tccattagat acctttatat taaatccaga taactttatt atcactactt gaaaaaatct
    11641 agatgttatt atccctataa ttattcctgt tatacaagaa cttattccta taagcatatt
    11701 ttctacaaaa tatatttttc ctatagtata taaatctaat cccataaaag acaatattcc
    11761 aatttcttta gacctattct taaaaaatat atttgttgca taccatataa agaaaaataa
    11821 aaatactgat agtatcatta ttacagctac taacactgat agtgtaaatt ttttattttc
    11881 atcttgtaac acatttatag catcaccata tcttaataat tcaaaattag ttagcacaac
    11941 tactgaaaaa accatagata taaaaaatgc tagataagtt cttatgttac tcttaaaatt
    12001 attataagca attccaagta tactcactta catctcacct cccatagagg ctagcatatc
    12061 cataattttt ccataaaatt cttttctact atttcctttg tttaatttac actttatatt
    12121 tccatcattt atcatatata cttcatttga ataacttgct gttagagtgt catgtgtaat
    12181 cattataata gtagcgttat tttctctgtt tattttttct aatgattcta aaagttcata
    12241 tgctgattta gagtctaaag caccagttgg ttcatcagca aatatgactg ttggatttgt
    12301 aattaatgac ctagctgctg cgcctctttg tttttgtcct cctgataact gatatggata
    12361 tttatctaaa tgattttcta atccaaattt tttagctata gaaaatacct tagcttctat
    12421 ctccttgctt ttcttttttc ctaatgctag aggaagtgct atattgtcca ttagtttcat
    12481 attgtctaat agattatagt cttgaaatat aaaccctatt ttttctcttc ttaattttga
    12541 tagttcatta ttttttactt ttgatatatt aattccatct agtattattt ctcctgaagt
    12601 ttgtttgtca agtgtagaca ttaaatttag caatgttgtc tttcctgcac cagatggacc
    12661 cattattgat ataaaatcac cttcattgac tgtaagagat atatttttta atacatttgt
    12721 tttaaatcct tttattccat aatcttttga tatatttttt atttttaata tttctttcat
    12781 aataatagcc tccagttgct aattttttaa tatatattta ttttaaaggt tttatataaa
    12841 taaatcctta agtaaatatt acattttaac tgttagcatt acacttttgt aacatatgga
    12901 agactattat aattataatg gaaatatctt aaaataaaag catgatttac ttctttgtat
    12961 aaacaaagaa aagaagctcc taagaagcct ctaatctata caattattct tctctataaa
    13021 aaaccaaaat acacattgat tgtggcaaat acagatacta taaacaagtg atattactac
    13081 tatatattaa aacaattatt cctgaagata attcaataag actatcaagc aataagctaa
    13141 gcatccaaaa agacttaata ccattaatgt ataattatac ccaatattaa tgcttaaaaa
    13201 accagcaaca gttggaataa tcagagatga acctcccata aatatactta aagctattaa
    13261 ttggctacta cttctaagtt ttttaggaga aacttcaaat attaatcttt ttgaagtaat
    13321 taaaattaaa ggagtagaaa acacttgaaa aacacacagt ataataatga tatttgaatc
    13381 atttgttaaa gcaaatagta gaaactgaac tgttatcata atagcactta attttaataa
    13441 gtttaaactg ctatattttt ttaataaata agaaccagcc agatatgttg gtatctctaa
    13501 cactgactgt aaagaccact ttaaagatat ctgagaattt gtagcattta aagctatcat
    13561 tttatctatt acagtacagt tatttgctac caccattgag tataataaga ataaaataca
    13621 tactagtaag atatattttt tatcttttaa taattcaata atatctttat tagttacatt
    13681 tattttctta ttaatctttt caatatcagg taataatagt atatttataa taagtaatac
    13741 tacaattaaa ccaatcgcta cagataatcc aaaatatgag aatctaagaa tcaagtaaga
    13801 tgctaccata cttccaatag cccatccaat cgacccaaaa gtcttaataa atgacaaata
    13861 attattaata ttatctccac aacctattat ccatgtatca tataaactac agcaagtatt
    13921 taataatcct cctgataaag caacaagtaa taagtatatt ataaaagttg cagattcagt
    13981 agaaaacata ataactgatg caattccata aactgataaa ctaattaaaa caattttttt
    14041 cattgtttga tacttatcag ctaatactcc aaataataat tgaaacaata tattggttat
    14101 tgcatagctc gataatataa ttcctctttc aaacgctgag taacctaatt ttgataagaa
    14161 aggtgtgaat tttgttgaag caaaggcaaa aataatatat gagaaaaaat agaatatgat
    14221 aaatttaaat ttaatcgctt tattaatcat aatcaatcaa aattgataac tttaatacca
    14281 ctatttcctt cctgatttaa gtttgaaaga tgatatttta ccgtattagg attctctaca
    14341 ataactaaag gaatatataa agtattttca agtaacttat tatcaatttc tactaacttt
    14401 tcccccatat taacttcttg accttgttta acttctaatt taaagttttc tttttttgtt
    14461 gttgcactat ccaatccaat atgaataaga atttgaacat cattttgcaa tttaattcct
    14521 atagcatggt tagtttgagc aataaaactt attactcctt tagtcggtga tttaagaata
    14581 ttgtcctttg ctatcatagc aaatccatct cccattacct tagttgaaaa tactttatca
    14641 ggaatttctc caagattaat tatttttcca tcaatgtagg aattaattgt aattgcttct
    14701 ttttttcttt taaagaacat tatagacact cctctacata attaacttta ttctaattat
    14761 cattacaaat aacgatatta agcatactgc aattgaggaa aaaagagcct tatagctctt
    14821 tccccattgt agttctttaa tactgcttat gatttgttta attccaacaa atttataaat
    14881 ataggtaaac atgataacaa tagttattgt gtaaatttgg aatatctgcc ctaataagac
    14941 taaatagaaa gcaatcatgt tttcttttat ctctttattc ttttttatat tgtaaataac
    15001 atatgcactg tatactgcac taaagttaga gattaaattt aaacttgcat taacatcatt
    15061 gtttaaaagt tgtgaaaatg aaatatttgt aaataatgat gtcaattcta aatacactat
    15121 atataaaaca ggtaatatta gaaaacaaac cataaccact gaaagaaata tattttcatc
    15181 ttttaatact gctgtgatat tttttatttt atttgttatc atgtttttgt tctccttatt
    15241 taaaagttgg ccagaaatcc ttattagcca caatcatttc atctaagatt tcttttgcta
    15301 cttttgcact tggaacagtt ttactcatag taaacgactg ccataatttt tgataacttt
    15361 tttctatcca tgcttctaca gctaattttt cagaagcaac ttgttgttcc attaaacctt
    15421 tttggaaagt tggaatttta cctatagaca taggttcaac catatctttt ccaacaaggc
    15481 aaggaatttc aaccattgca tcatctgcaa aattttcaat agctccatta ttaggaacaa
    15541 tcagtaaaaa tctttcctga gtattaaatg ctaatgcaca agctaattca acaataaatt
    15601 gagaatgttc acttgcatca aaccatgtat ctcttgctgt gccatttttt ataattcttt
    15661 cacattcttc gaatgtatcc ttttctcttc tatccataat ttcatttgct cttgtataat
    15721 ttggattagc atgttcaaca gtttcatcag ccattaaata atattttaaa taagtattag
    15781 gtaatgtagt cggttctatt tccaataaat cctttgctgc tctcatagtt tctaaccaac
    15841 tagattcttt gtgttgacct tgaggtgtaa attcctcata tcctttttta gcacaatatt
    15901 cttgtacttt aggcattaaa tcatttcctt ctttgtcctt aatacttgtc caccacccga
    15961 aatgattcaa tccataatat ctaacatcaa agtctttacg agaatttaaa cctaaaactc
    16021 tagcaaatat ttcctctaaa catactggca tatcacaaat attaataatt cttgaatttg
    16081 gtcttaatac acgacatgct tctgcaacta tagctgctgg atttgaataa ttaagcatcc
    16141 aagcatttgg agaatatttt tccatataat caatcatttc gattattgga cctattgttc
    16201 ttaatccata ggcaattcca cctgccccac aagtttcttg accaacaacc ccatatttta
    16261 gtggaatttt ttcatcttgt tcacgtaaag catataaacc aacacgaatt tgtgccatta
    16321 caaaatcaat atcagtaaat gcttcttctg gatttgttgt ataactaaat ttaatttctg
    16381 gatttttctc tctaacaatt attgcacatg cttcagcaac ttttctttgt ctctcatcat
    16441 tattgtcata taatttaatt gattcaatag gaaatttagc aacactttca attaacattg
    16501 ttacaattcc agcagtataa gtactacccc caccagcaat tagtatcctc tgtctttttc
    16561 tttccatctt tccttctcct ttataataaa ttttcaaatt cttctcttac acttggtact
    16621 gaaagcccaa taataatctg aatagctttt ccattatgca ctaaaccatg tgcacctact
    16681 gccttaaaat cttcttcttt agaaataatt gagtcatctt tcaaggttaa tctcaatctc
    16741 gtagcacagt ttgtaacatc tacaacattt tctgctccac caactaattt tatgtattca
    16801 gatgcttgtt gagaggcttt actcaattta tttccttttt catctttagt ttctaaatat
    16861 tcatttttag tatatagttt tgtttcttca ctatcttcct ctctaccagg agttttcata
    16921 tcaaacttct taattaagaa agtaaataca aagaaccaga taaatgaaaa tataatccct
    16981 acaacaactt gaacaacata taccttccag tgatacattc caagaggaag ccaatttaat
    17041 acaacccagt taataattcc tccaccaaaa tcaccaacaa ctcctagggc aaacgaaatc
    17101 gtttgcaagc atgcatctaa aaatgcatgt actaggaata aaagtggtgc tacaaataaa
    17161 aatgtaaatt ctaacggctc agtaattcca gttaatactg cagtaagagt agcaggaatc
    17221 attaaaccta atactttttt ctttttttct ggctttgcag ttacatagaa agcaccaaat
    17281 acacctaatg aaccaaacac cttcgataat ccggataatg aaaatccgat tggatacatt
    17341 tcctttagag tcttagctga agtttgaaag tcctgaatat gagtagccca atatactgat
    17401 gtaccacctg gaacaacaac actatcataa caaattggtg catatacaaa atgatgtaat
    17461 ccagttggta ttaaaatctt ttgtaagaat gcataacacc aaactcctat atttccacta
    17521 tttataaaaa acgtttgcaa atgtctaata acattttgaa tatgaggcca aataaaacaa
    17581 gttaataatg caactggtat cataacaaag aaacaaattg caactacaag tgatgagcct
    17641 ctaaataatc caataaaatc tggcagttct ttatcataaa atctattatg aatataaatg
    17701 acaatacctg atattaacaa agcaccaacc ataccagtat ctaatgtttt aataccagca
    17761 atgcttacaa gtccacttcc agcacttatt tcagctgcga aatcaactcc aaagcttttt
    17821 ccccaaaaac ttaacataga acctacaaaa taattaaaag tcaaatacgt tgctaaagcc
    17881 tccatacaag ctcttgcttg ttgctttttt gcaagtgcta ttggtaaaga aatagcaaat
    17941 aataatggta attgattaaa taccatccaa gctccactag atattacacc ccaaaattgg
    18001 taccaaatat tttctggtgt agcaagtgaa cccataattg actgattttg aaatacaatt
    18061 gagaatccaa ccattatccc agcaaaagca aataatagtg ttggtgtaaa cattgcccct
    18121 ccaaaacgtt gtattttctt catcatataa attactcccc tcctcatatt tttagtttat
    18181 ctttggcctt agatatctat tattttaaca ggttaataaa tatatacaat ctttagggta
    18241 tattcggagc ttattcacag tcaaataagt ttttgcacat ttttatgttc aaattctcat
    18301 aaaaaaaaga atgactacaa tttgcaatca atcccttctg taatcattct atgcctttta
    18361 taaatactat atttaataaa taataactca atcaatataa aatataacat tgtcaattta
    18421 taactagggt gcatttgtaa aaatgagata ttagtcgctg aaatataaat tgattcatca
    18481 cttaaatcag tcaatgtatt atttttaaac tctgtaattg aaattatttt agttcctatt
    18541 aattttaatc ttctagcaat attaagtgta ttctcattct caccacttag ggatataata
    18601 atcataacat catttttact gacaatattg taaaaagaat catcaaatgt tcttccctca
    18661 aaatcatata ttatttcttg acatgataag aacattctct ttatctgttt tgcaaccaca
    18721 ttttgaatat cacctgaacc ataagcgtat attgttcttg attcataaag aagtttacac
    18781 aatgcatcat aatctttatt tttttgatct tcaacatact taattattga attacatgca
    18841 atatcaaatg actgattttc tatgacatga accttttctg attcccagct taataatact
    18901 ttaaactcac tgaaaccttt taaatctaat tttttaacaa atcttgtaat agttgcacga
    18961 gacacattac atttcatagc aagttcattt attgacatct tactacaatg ttttttattt
    19021 gcagttatat atttccatat taataaatct gtatcattca atttattata attaaaaaaa
    19081 atcatttctt ctaatctcat aaactattac cccctttaat taaaaatcgt cataaatatt
    19141 ataccaatat tcaacattaa attacagcgt attcttatat ttactattta gcccataaaa
    19201 ttgactataa aaaatactgc tataattgat gtttaaaaaa atgtagtttt aattttattc
    19261 ttttatctaa taattaatat tttcaagttc tagtgctttt cttatacaat tatcatcttt
    19321 aaaatcagca gtccttttac aatcttttac tttaaattct ggaatagttt taaattcctc
    19381 atcacatatt ccattcttat ttaaatacat acaacttgcc attctaacta ttagaccact
    19441 attttttaaa ttaaacaata cagggtctat acaccctcca tctccgccag tagtttcacc
    19501 aactaaagtc gcaaatttat tttctttaca aaacattgca aacgactctg atgatgaata
    19561 tatttttcta tcaacaagta agtatatatt acccttaaat tttgaatcat ctgtagattt
    19621 tatagtatag gaagtatctt caaaatcatt aaaatctttt ataatttctt taggtgcatt
    19681 ttcctttaca cttgctggta aattttctac aggatttaat tttatatttc ttgcatttgt
    19741 atattttttt actatttcat tattatttct ataaattcta taaccattta ttttgacatc
    19801 attttttatg agtttagata cgattccctg ccaataccta tcacttccac ctaaattacc
    19861 tcttatatca ataattaaag ctttatgttt ttctaaagtg ttaatataat ctccaattat
    19921 ttttaaatca ttatttatag agccattctt tgatgacatt gatggtaaat acatatatcc
    19981 aatctgaccc ttaataacat cctttaaaat taattctttt tttacaaaaa tatcatctgg
    20041 catttttatt ttaagattca tagagtcata tctatccaca acttttttat cattaagaaa
    20101 atcataccaa tcattattag agtatgattt cttaaaaagt tcatatcttt tcttattatc
    20161 tattacttct gtgtgtttat tatttaaatc agataatata gatgacagtt cttctataaa
    20221 ttcttcatca ttttcagtgt tttttattcg ttttgtatat tcatccttat ttgctaacca
    20281 atcaatatta ttaagcctct tatttacacc tagatatgga tatcctgtct ttacaacatt
    20341 atacacataa ttaaagtcat ccaccttttc ctcttcagac aactgattct tttgagaata
    20401 agtagaatta cctaataata ttataaatgc taaaataatt actaaaaata atactttctt
    20461 tttcatgtaa ttctcatcca ctttcagatt ttatatttta atacattgta tcattatttt
    20521 attatcacct aaacagttaa aagaaattct tgaagatata ttccactatt attctattta
    20581 aaaattttta tttgtatttt caaaattatt tcatgaaagt tataaaataa caattatatt
    20641 caaatatcta ttttactgct atatcctgaa agtaaattct tatcagtgat atactaaaaa
    20701 agatatgcta aaaaatttat ccattataat gacttaaatt tagatacttt actgattttt
    20761 atttattaat tgagaaagat attgataatt taaaatattg ataactatag aaatgattat
    20821 tttttataga aattttaagg atataaacta attatactta agtctatatc cttaatttat
    20881 catgattatg agaattttta caaatactta tctttgtctg ttttagaata aaaacaagaa
    20941 catatcctgt tattttattt aaacatccaa gtttaattac tacacaatta cttattagaa
    21001 attgtatcta cttcatatat atatctgtta agtatagctt ctaacatttc ttgtcttcca
    21061 gacttattct ttataactgg catttgcagt gcataagatt ctaactcttt aaatccaacc
    21121 ttaccttcta ctatatcctt acctatacct tcactaaaac ttgcatatct ttctttagta
    21181 aagttttcaa atacctcatc ttctaataat ttatgtgcta ttaataatcc ctttgcaaat
    21241 gtatccattc ctgcaatata agctaggaat aagtcttcta cctcaaaaga tgctcttctt
    21301 actttagagt caaaattaaa ccctcctggt gctattccac cttgctttaa cacttcatac
    21361 atagcaagtg ttgcatcata tatatttgtt ggaaattggt ctgtatccca acctaataat
    21421 aggtctcctt gatttgcatc tatactacct aaaacattgt ttattcttgc tatatttaat
    21481 tcatgttgga aagtatgtcc tgcaagtgtt gcatggtttg cttctatatt cattttgaag
    21541 tatttatcca gattatactt tcttaaaaat cctaaaacag tagcagtatc aaaatcgtat
    21601 tgatgtttag taggctcttt tggcttaggc tctattaaga attgtccagt gaatcctatt
    21661 tccttagcat agtctacagc catttgtaag aatcttgcaa agttatccat ttcaagttct
    21721 gtattagtgt taagtaaagt ttcataacct tctcttccac cccagaatac aaaattttct
    21781 cctcctaagt acttagttat ttccatagct tttttaacct gagctgctgc atatgcatat
    21841 acatctgcat tacatgttgt agatgcacca tgaacaaatc ttggatttcc aaacatattg
    21901 gcagttcccc ataataactt tatacctgtc ttttccatat taacttttat atactcaact
    21961 atttcatcta atatcttatt tgtctcttct aaatctcttc cttctggtgc tatatctctg
    22021 tcatgaaaac agaaatactc aattcccatc ttagacataa attcaaatcc tgcatcaact
    22081 ttaatctttg ctatctcaat tgggtctgtt atattattcc aaggtctctc tactgtgcct
    22141 actccaaatg ggtcattacc ctctccacat aaagtatgcc aatagctcat tgcaaatctt
    22201 aagtgttctt tcatagtctt attacctacc acttgttcag gattgtagta tctaaatgct
    22261 aattcattgt cagattttac tccttcaaat tttatctgtc ctattccttt aaatatttca
    22321 ctcatttcat gtcctcctaa tattttacac atataatata tgtagtatgt tttcttaatt
    22381 taaaactatt ttctatcttt atttttcttt ttaagctaat ttaattattt atttttgcat
    22441 taactacttc atctaataac ttaaagtcat ctttcaaaga tttgtatatt cttttataca
    22501 atctatgata tatattgtat gtatctacat tttcacttat tggttctaat ttagttgaag
    22561 tttttattat agcatcacaa gctttttgta tattttcata tattccagct cctactaatg
    22621 caagaattgc aactccaagt gctggtcctt cacttgaatt tatagtatat acatcttgat
    22681 taaacatatc tgcttgcatc tgtctccata ctttactttt tccacctcca cctgaggctc
    22741 ttacttcatt aacgtctata ttaagattct ctattatatc catacagtct ttaagactat
    22801 atgaaacacc ttccattatt gaccttgcga tttctttttt agaatggatt ggagataatc
    22861 caaagaacac acctcttgca taagggtcaa gatgaggggt tctttctccc atcatatatg
    22921 gtaagtaaag caatccatta catcctgttg gtacttgact tacttgatta tttataatct
    22981 catatacatc ttcacctaaa gaatttgaaa cttccacttc actttgacag aaattatctt
    23041 taaaccattt taatgacaag ccagctcctt gagtaactcc cataacatgc catgtatttg
    23101 gtattgcatg acagaaagta tgaactctac cttctttatc aatttttggc tcttttgtat
    23161 aagcaaatac tacacctgat gtacctatag tagatgaaac tactccatct ttaactattc
    23221 catttcctat agctcctgct gcttggtctc cagctcctcc taccactatt gttccttctt
    23281 taagccctgt caaacttgct acatctctag tcacataacc tgtaatctct tgtgattcat
    23341 aaactttacc taaaagattt ttatctatat ttaacttttc tagtacttca tcgctccaat
    23401 ctctttttct tatatcgagc aactgcatac cacttgcatc tgatacctct gtagcatatt
    23461 cacctgtcaa tttgtatctt atataatctt ttggaagaag tattttatta gtatttttat
    23521 atatatctgg ttcattgttt ctaacccata atatctttga tgctgtaaat cctgtaagtg
    23581 ctggattagc agttatttca atcagtcttt cttcacctac taaatcagtt atctcctcac
    23641 actcttttac tgttcgttgg tcacaccata ttatagagtt tctcaatgtt ttaccttcat
    23701 tgtccagcat aactagacca tgcatctgcc ctgataatcc aattcctttt atacattcac
    23761 tgcttatatt tgaactttga actacttctc ttatagattc gactacagct ttccaccaat
    23821 catttgcatc ctgctctgcc caacctgatt tctcttgtat aagaggatat tcatatgtac
    23881 aactttttat agtatttcct agtttatcaa ataaaactgt tttagtacct gatgtaccaa
    23941 tatccacacc taatacatac tccaacttca tccctccttg aatgccactt cataactatc
    24001 gttttcctat cctaattttt ttatatttta ggtatatttt acttgcttta tttttgttag
    24061 tatattaaac taacttatga actaataata aaatatttaa ttatctttgt caatgcaaaa
    24121 taacctttta tttttgtgta aaaagtatta caatattatt ttaaggagga atatcaaatt
    24181 ggttactgat aaatacacta ttcgagaaat gaatgaaaga ttggtacttg aacaaattat
    24241 aaaaaatggt cctatttcta gggcaagtat agcttctact ataggattga ataaagcaac
    24301 tatatccgct attaccaaaa aacttattga tgaatctcta gttcatgaaa taggaattgg
    24361 aaatagtact catagtggcg gaagaaaacc catattatta gtatttaata aatgtgcagg
    24421 gatttcttta agtatggaca ttggatacga ctatatattt tcttctctat cttacttaga
    24481 tggaactatt ataaattcta agaaattaac agatatacaa gtaagtaaag acaatgttat
    24541 acaattgata gatgaaatta taaatagtta taacatatcc aaaatagaca ctccttataa
    24601 ggtaattggt cttactttgg ctatacatgg gattacttgt gaaaataaag tattgtttac
    24661 tccatattac aatcttaatg agattgactt atatagcatc cttagcaaaa agtatgactt
    24721 tcctattcac atagaaaatg aagcaaactt aacagcttta gctgagaata ctttttcaac
    24781 tgtacataac tcactactta gtctcagtat tcacagtgga tttggttcag gcattataat
    24841 aaataataaa ttatatagtg gcagaaatgg tatgagtgga gaaattggtc acactatcat
    24901 catgcctaat ggtaaactat gtccttgtgg aaatcgtggc tgtttagaac agtattgttc
    24961 agaaaaaaag gtatttgaac agctttcttc tttagaaaat attcctaaaa tagattctga
    25021 tatagtaaag caactatatt atgaagataa ccagaatgcc aaaaaagtta ttcatgagtt
    25081 ttgtagttac ttaacaatag ctataaataa tgctattaca acttatgctc ctgaaataat
    25141 atatttaaat agccaaataa ttagtgatat ccctgagata ttacagatta caaaagatat
    25201 gttagttagc tcttttaaca aaggaataaa tatagaaatt tcatcattag ggtctgaggc
    25261 atctctatat ggtggctctg ccgtaaatat caagagcttt ttgaatatac aaaatttaac
    25321 tttaattaat gaaatataag ggttatgact atatacagcc ataacccttt aaattataca
    25381 ttctaaaatt agatttatta tatagtttat gcaagattag ctatattttt taaaaattgt
    25441 atatcaatta aatattaaca taagtatttc gttttaaata ttaatataat cttcctactc
    25501 atcatcagat aataaacttg cattttgttc aaccatagaa atattatctt tacctatttt
    25561 ctctaatttt gctgccaatt cctttgtatc taaaaaagct gattcaataa ccatacctaa
    25621 atttagccct gaaataattt taattcgact gtcattattg gctactaata aagcagcatt
    25681 gcatggagtt ccacctagta aatctgccat tataattatt tctgaacttt ccttgtacac
    25741 ttctaaagct ttttttaatt tttctattgt tccttctatt ccatcatctt gtttcatatt
    25801 tacagtagga taatctatag aatcccctat tatcattttt gcagaattta agacttcttc
    25861 agccatattg ccatgactca tcaaaataat actattcaat ttaatacacc tctttaaatc
    25921 tataagatgt ctacaagata agttttctta tcatcaggcg tcattatagt tgtaatttca
    25981 attccttcat ctaaaagtct tttaaatgct tctttctctt cttcagtaac actgatatta
    26041 ggtcttagaa ctgttgtatc ttttctactt gacatatttc ctacatttat attatgtatt
    26101 ttaaatcctg cctctacaaa tggaataagg tccacaggag acttaacaat taataataca
    26161 ttttgtcctt catactttcc tgcagtaata ttttttatag cagtctcttc tgtaattata
    26221 gatgacgcta tacctgcagg tgtagccatt cttaaaactt gtttttgaac agaattgtta
    26281 gctactgcat catttactac cattaatctt gtaacaccca atttattact ccacatagtt
    26341 gcaacttgac catggattaa acggtcatct atacgaatat ttttaatacc tttcatttcc
    26401 atatctaata tcatcctttc cttattttaa aatacctaat gcatataaaa ttattgaaac
    26461 aaccattact cctaaaatta agtatatagg ttttatattc tttttaagag cccaataaac
    26521 aatcacaact agaattgcag gaactaatcc aggcataatt tggtctaata tttcttgacc
    26581 tttcattgta aaatcacctt gttgaaagtt caaagcaaca ttagctttta caactgaagg
    26641 tattaatgca cctacaacca ttaaacctaa aatagatgta ctctcagtta aaatctttaa
    26701 acttttttca attgaagata ttagcttagt acctgatgta taaccagcat taaatagtgg
    26761 tatacttaat aatttcaatg ctacactcac tacaatccac agtaagattc caaatggact
    26821 tccatctatc gccatattag ctgctatagc tccaaaaatt gtccacggaa taacgacaaa
    26881 taaactatct cctaaacctg ctactggtcc catcaagcct gttttaattg aagcaacagc
    26941 ctcttcagtc tcttcacttg ataactcctc cattgctaca ttaataccca aaatataagg
    27001 ggcaacccaa ggattagtgt taaaaaattg taaatgattt aatacagcac tttgtaactt
    27061 ttctggatta tctttgtata ctacttttaa aaaaggtagt actgcataac agtatgccaa
    27121 ggcctgcatc ttctcataat ttgaagaaac agaactagta aaaaaccagc gccataagac
    27181 actttttttc accttttcat ttgatttatt taatgatacc ttatcactca tcttcgtcca
    27241 tacctcctac tacagtatat tgttgttctt gattttccaa attctttttg taaattataa
    27301 gtgcaattgc tccaccaatt aaagctacac ctaataatgg tactttcata tatactgcta
    27361 aaacaaaacc aattactaag taactaaaat actccttagc aggaagatat ctaagtaata
    27421 atcctatccc tactgctgga agtagtcctc cagctactgt taaaccacct gttaaccatt
    27481 caggtgtata tttcaaaatg gtattaatta aagaaggtcc aaatattaca gatagagcaa
    27541 ctggaatacc tgttgtagct gctgttaata gtactcctaa catttgcata taggttatat
    27601 ttttataatt tcctttttta gctccatctt ctgctttatg aactagccat ataccaatag
    27661 tatttctaag aacatctaat tgtaccatca ataaagcaac tggaatacct atagagattc
    27721 ctacagatgc ttcttgctta gtcgtaatag ttataaatgt ggctactata cttgctgttt
    27781 gataatcagg tattgatgct ccaccaaagt tactaatacc taatgataat agctgtaatg
    27841 tacctccaat atataaccct gtatttatat ctcctaagat aagaccagta ataaaacctg
    27901 ctgttactgg ttgatatgtt ccaaacattg ttgaattttt ttcatagttt ataaaaaatc
    27961 catatagtac gattaataat aattgccata attgtggctc cattatgatt tcacctctcc
    28021 caaaatattt tttagttctt taaaattata actggaatct tgctttagga aaataggaat
    28081 ttcttccagt gggcattcaa cttcataagt tttcccacct tcataactaa ctccactaaa
    28141 tatattttcc catgtatgtc cttttggtaa atatactttt ctacttctat ctttataatt
    28201 cattattgga gctaccaaaa tttcatctcc aagcatatat gtattatcca cctcccaagt
    28261 tttatcatct tcaggaaatt cataaaataa agttctcata actggtgttc catcttcatg
    28321 cgcttctttc atcagttttt ttaaataagt ttttaagctc tctctaattt ttatgaattt
    28381 tgtaagaatt acttcaactt cttcaccata agaccatatt tcattaggag ctccagttac
    28441 cattgagcct ccacctttat ttgataatgg tttactatga ggcaatctat ctccatgcat
    28501 tctcaaaatt ggtgaaaaag tagcatattg gaaccatctt accataagtt ctctaaattc
    28561 tgggtcttta ggatttcctc catggaaccc tccaatatca gtagtccacc atggtatacc
    28621 tgccagtccc atatttaacc ctgtattgac ttgattatta aaagcctcaa aactagaatc
    28681 aatgtcacca gaccatacta aagctccata cttttgagct cctgcccaag ctcctcttac
    28741 aagagttaca acagactcta tgtcattatg taaaccatca taaatcattt ttaaataatc
    28801 aataggataa atattaccac aacttaaaac atttcctttt ttatatctat agttatcaaa
    28861 atcatacaca gaatagccag gttcagctac atctaaccaa taatagtcaa tcccaagttg
    28921 tttataattt ttatctattc tatcccaaac atattctctt gcattttcat ttgtcatatc
    28981 tacaaagact gtatttcctt gaatttgcat tgtcattcta acaccacgat taacattgac
    29041 taaatagcca ttttctaaat aatcattgta gttttctgca tcactttgaa ctgtaggcca
    29101 tacagatact attggttcta ctgaatactc ttctttcaat tttttaacta attctttagg
    29161 atttttccaa taatctaagt caaatttata ttcaccttgc ttaggccaat gaaaatagtc
    29221 aattgcaatt gatgaaagct taattcctct tttagaatat tccttcacaa catctaaaac
    29281 ttcttctgaa gttctatatc ttagtttact ttgccatagt cctaaaaggt tttcaggcat
    29341 cataggaaca gttccagtaa cttgagaata atttttattt aactcttttg ggctttctcc
    29401 acaagtaatc caataatcta taaaatttgt agaaaacatc ttccattctg tcatattttt
    29461 agcaaaagat acttttccaa ttcccggatt attccataaa agtccataac ctagactaga
    29521 aatataaaaa ggaactgata tttgagaatt tctttgggct aactccaaaa cagtattctt
    29581 taaatctaaa aatttatgtt gatattgtcc cattccaaaa atcttttcag aaggctcaga
    29641 ttcaaatcta gtagttactt caaattctcc actatagctt ggtcgatatt ctcttgattt
    29701 taactttaga gtagagttaa agtctttggt aatctcaatt gtccctacat cttctccacc
    29761 atcatcatgt tttacagctc ttaatcgaat atattctttc aataatattt catttttatc
    29821 attataaaaa gtaattctat ctctatggtc taagacagct tttatctttc catttttaat
    29881 gattgcactt ccatcttcat ttttattaat tgtaatatca tctttattta acttaggttt
    29941 ttcgttaaga gcatagtttt catcaacaaa attttggtct acaaacgacc taatacgtaa
    30001 agagtttttt ccccatgctt ctattcgcaa tagttcattg tcaaatcttt tttcaatata
    30061 gtcttcagat atttttatca cggctacctc ctaaatcaat ggtataatat tctatctttg
    30121 tgtagcttaa gttaaataat gtcgtttagt atcgttattt ataaattagg ttttatatat
    30181 aattcatttg tacattaaac taacttacca ataatatact ttatttgact ttgtttgtca
    30241 atcgctttcc ggattttagt aaaattttct aattaagtaa tagaaagtaa ttcttaatat
    30301 tttttattta tactttttaa tatctgtgat aatatttata tacattatat attcattgaa
    30361 aatcaaaaaa ttgtttttta ggagtctatt atgaatacac ttaaacatga atcggtaaat
    30421 atccatgatg atataggtgt aaagctctat ctatctattg ataatggaag ttttacagtt
    30481 aaacactggc attatagtct tgaagttatt ctcatactta atggaagctt taatcttttt
    30541 atagataatg aaagattaaa tttaaaggaa aatgatttaa taatattaaa tccaaagcaa
    30601 attcattctg ctatttgttc tgatggtaat gaatctatag ttcttcaaat tccttataaa
    30661 tttttagaag aaaacatacc taatataaat gagttacgta tt
//
LOCUS       DBGJFF010000036        30697 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.036, whole genome shotgun sequence.
ACCESSION   DBGJFF010000036 DBGJFF010000000
VERSION     DBGJFF010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30697
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.036"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            120..2021
                     /locus_tag="ACOIAF_001673"
     CDS             120..2021
                     /locus_tag="ACOIAF_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HGZ2668687.1"
                     /translation="MRERIILETLKNSSGYLNIDYFADKLGVSTRTIRNEIKKIETIK
                     ECNGFKLEYKSKLGYILNIKDQDKFEHYLRNLPSDLVENPEQRLESIIVELLVNEGYK
                     TIEQLSKKFLVSSSQIKNDLKKIDEKIKDTELKLERKAHYGIKIEGSIKEIQSILVDS
                     YFRGNRNITEYRNKFIDNLKLANIRSTIKNVLNEHDLEANLTELEEILAQIIILYIRV
                     SMRVAGNASDLKLYTEDLIIDKLLDKIFRDKKYNLNYDEKYYLKELIKLKTKDKKATI
                     KNIDKNKLQDIMFEFFREIDKKYNSNFLEDKEFFNLFYLHIACLIERIKKNHKIINPF
                     SVKISQQYPTVFNLSIQFSKIIENEYHIKISQDEIGFIATHIAVPFEKREEANFNKKY
                     KIAIICSSGGGSAFLINLRLKEIFPNAEIRNFSLLEEKAVIEFAPDLIFSITNLLFEI
                     NAPVILINEILDELDYLDIKESVRFADHIGNISSPKQYILGLFDKNHFRCIKDKAKYK
                     DILDSMSKCIVDEGACSPTYPKDVWERESYLSTIYTNGVAIPHPIEMTGNKNIISVAL
                     IQTDIIYENRVPKIIFMISLIKGNLELHKQISKYLSKIMANKDMVDMLNKSQSYEEFM
                     YKLKIYLGG"
     gene            2024..3067
                     /gene="ypdE"
                     /locus_tag="ACOIAF_001674"
     CDS             2024..3067
                     /gene="ypdE"
                     /locus_tag="ACOIAF_001674"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HGZ2668688.1"
                     /translation="MNIEFFKRLSEADGIASNEEEVRHVLLEELKAYSDKIICDGLGS
                     IIFSKIKDESAPNVMICAHMDEVGFMVRSIDKLGMIHLITIGGVKPLAQFVQKVRITT
                     KEGKKIPAVINATYNNGKAENIYADIGAYTEEDVYNLGINVGDMVTYTTSFEEFTLPD
                     RLVGKAFDDRIGCFVMGEVLKELRKENLNCNIHFAATSSEEVGIRGAKTSTQLINPDI
                     VFVIDVACAKNEFVRDYTNQKQIDKGVMLMHRDRTLVPSRKMIDYVMEIADKNNIPLQ
                     HDMFESGGTDGGEAHLVNEGKPCVVTCVPVRYGHCAFSIVSNKDLENIIRLYTQLILN
                     FDEEQYKHIRNFI"
     gene            3082..3387
                     /locus_tag="ACOIAF_001675"
     CDS             3082..3387
                     /locus_tag="ACOIAF_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HGZ2668689.1"
                     /translation="MSTEVQIMELISTAGESKSKAFEALKKVKSKDFKGAKLLIEEAR
                     KIDIEAHRIQTELITQDLNEDVESPAMSLLMVHAQDHYMTSQLAKDLIEALIYCLDK"
     gene            3484..3819
                     /locus_tag="ACOIAF_001676"
     CDS             3484..3819
                     /locus_tag="ACOIAF_001676"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGZ2668690.1"
                     /translation="MKILLCCAGGFSTNMLMQNMKKVVKESAKLNEKDFEFTAIPADS
                     IESEVDDWDIVLIGPQIAHKIDFLKGILEPKNKPFAVIDKDVYGKMDGATVMKLALIT
                     YKKHQLAQG"
     gene            3851..5173
                     /locus_tag="ACOIAF_001677"
     CDS             3851..5173
                     /locus_tag="ACOIAF_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HGZ2668691.1"
                     /translation="MFSKFEAFMNRVFMPLAHKVDNQRHLGSIKAGMVAMTPFTILGS
                     IFAILPALPNMLGENNPISKFILNNSAILDLPVSLSIGLIGLYACMCIAFNLGNYYKL
                     YIPGCVALSTFSFLFLVASFTPDGALEIGNLGAKGLFTGMIAGLGSVELYNFCNKKNI
                     TIKMPENVPDFVSRSFELIPTTIIVCGTFIVARFISLKVFGELPPQILTQFLSPLVGS
                     MDNPWSVLALNFAICTIFFFGIHSSVFSPITRPIMITFIAENIAAMQAGETIPHFFTA
                     GASSAFFGFTGCGISIGCVIACMLSKNASYKKIGRVSLFPALFGINEPILFGAPIILN
                     PIMFIPHVFGGAIIGTLPMFFMHWGWLAKPVFDPPYVGVFLEGFLTNGDWHSILANAL
                     QLVLAVLLYWPFFKIMERQQNNKKQKEETKNIFSKEEEDLLDDLGLDF"
     gene            5195..6253
                     /locus_tag="ACOIAF_001678"
     CDS             5195..6253
                     /locus_tag="ACOIAF_001678"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HGZ2668692.1"
                     /translation="MERLVSLIYKRGVDALLIKAKNTKRYIGALSGSGVYVLVTKDKN
                     YQILDGRYIDEADKKTTGFIKRIVSQGSYIPTIIELLKELNIKKLAIENQAMSIQEYL
                     SLKNEGFEINLITNELWKLRAIKSKEEIELIKKACEITDEVFDEVISEIKKDMTELEV
                     SALIQYHALKKGASGMSFETIVVSGERGAMPHGKPTNKKLKVNEAITIDFGVVYQGYQ
                     SDMTRTISIGKPPKIIKEIYDVVLEAQLSAIESIKEGTRASDVDKVARKIIDKHGFGE
                     YFNHGLGHGIGLGDGEVPTLNSNSEDILVEGMVMSCEPGIYIPNVGGVRIEDDIVIID
                     GKGVPLNKTSKEFIILGE"
     gene            6258..7445
                     /locus_tag="ACOIAF_001679"
     CDS             6258..7445
                     /locus_tag="ACOIAF_001679"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HGZ2668693.1"
                     /translation="MKYNFNKVLNRKEGNCRKWSNHVIKEKFGLNEDAIPMDLADIDF
                     ECAPAIKESILKRASVGDYSYTFIGDDFYDAIINWNKRRFDVYIEKDWIKLTFGTVST
                     LHYIVQAYTKEGEGVLINTPAYDPFAEAVVNNNRKLCCSSLKLDNNRYYLDLEDMENQ
                     MKYENIRLLIFCSPQNPSGRIWTKEELYQVSELCLKYNVILVSDEIHRDIIFKDYRFV
                     SLWNAHPEIYKNSIICVSPNKGFNLGGLKTSYVLIRDVKIRETLLERLKSNSITSPNV
                     FAIPAIVAAYNESEEWLDAMTSYVEENFEIVYDFFETNIPKAKVMKSDSSFLAWIDVR
                     EVFRNEEESKEFFRHANLTMVVGSYFVKDGDGFIRINVGCPRETLNEALNRIKKTYIS
                     MYC"
     gene            complement(7642..8514)
                     /locus_tag="ACOIAF_001680"
     CDS             complement(7642..8514)
                     /locus_tag="ACOIAF_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HGZ2668694.1"
                     /translation="MNILQNENLIIESSSFGAELTKIFSKKYDKNILWDGNKKYWGRQ
                     SPILFPIVGKLFDNETIIEENLYSMTQHGFARDMDFEIVDKGDTFVTYKLTDNESTLK
                     KYPYSFELIINYTLNENSIEVEWIVKNTDSKDIFFSIGGHPAFNLPFYEQNDFSKHYL
                     EFKSKNDVEKINLNGSFTDDIIPIGKLKNLQLNPEVFKNDALIYTNIDEVSICNNDGS
                     KYVTISMEDFPLVGIWTPYYSETNSTAPFLCIEPWYGLADSINSNKIYKDKKFINKLS
                     KGKVFTASYNINIH"
     gene            complement(8908..9099)
                     /locus_tag="ACOIAF_001681"
     CDS             complement(8908..9099)
                     /locus_tag="ACOIAF_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668695.1"
                     /translation="MDDFSKLKSLYEDGYRCIYHDCVDNNYTIYLKNFYTEGSETIEL
                     SSESDFSQFKDYIDGLRMS"
     gene            9319..10365
                     /gene="cobT"
                     /locus_tag="ACOIAF_001682"
     CDS             9319..10365
                     /gene="cobT"
                     /locus_tag="ACOIAF_001682"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HGZ2668696.1"
                     /translation="MSLLESISKNIYSLDNSSIEKTKQRLDRLIHPTGSLGKIEDICM
                     QLAGIFGNENFDTSKKVIIAFAGDHGVYEEGVAPDPQNITKLQFPNFSKGLCGVGVIS
                     KFVGADVVAVDVGINCDEKLDGVLDYKIRKGTSNMAKGPAMSKQEAIRCLEIGIEIAE
                     QCIEKDYKVIGIGEMGIANTTPSTAIISVISGCDPLEVTGIGAGLKKERLKHKAEVIR
                     KAIEINNPNPTDGVDILSKVGGFEIGSMAGVILGCSANRIPVVIDGFISYAAALIAYK
                     INPKTREYMIASHLSAESGTKRALDILKLDPLLNMDMRLGEGSGAALAFDIIEASNYT
                     YKNMATFDEIDMGR"
     gene            10397..10957
                     /gene="cobU"
                     /locus_tag="ACOIAF_001683"
     CDS             10397..10957
                     /gene="cobU"
                     /locus_tag="ACOIAF_001683"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HGZ2668697.1"
                     /translation="MSKIILVTGGARSGKSNFAEKLCFDRNNNTAYIATSIPFDDEMK
                     DRVKKHQENRPRNWNTYEIYEDIYSIIKEIYKEHETVILDCVTLLVNNLMFEHNLNIE
                     ESSQEEINKLEQYIKEQVVKLIEEIEKTNLYFVFVTNELGMGIVPGNKLSRIYADIVG
                     RINQYIASKSNEVYFVVSGIAMKIKE"
     gene            10967..11740
                     /gene="cobS"
                     /locus_tag="ACOIAF_001684"
     CDS             10967..11740
                     /gene="cobS"
                     /locus_tag="ACOIAF_001684"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HGZ2668698.1"
                     /translation="MKRFILILQFLTRIPIKLNVGFDDEFYKSIVYFPLVGFVIGILS
                     YLIGWISMLLFEPFIASIIITLAGVLITGGLHIDGLGDTFDAIYSYRDKEKMLEIMKD
                     SRLGTNSLLAIMFVLLLKVGFVYDIISNNSLWVIIFMPMIARLGVMLLTYKTVTPREK
                     GMGNLFIGKLTTSMLITAIIYTLLIVALITKFIFLLPNIVLIKVLGSIIVVFVFIILF
                     KKHIYKKIDGVTGDILGCGIELSELVYLIYIYLLIFMFF"
     gene            11760..12374
                     /locus_tag="ACOIAF_001685"
     CDS             11760..12374
                     /locus_tag="ACOIAF_001685"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HGZ2668699.1"
                     /translation="MIRLILIRHALTNDNKKERLSGHINSCISEEGKLQINKITRYLS
                     NENIDKVYTTPSTRTKDTVEKISKLKLLEIEEKEALREISFGDFEGRTFEEIKIKYPN
                     EFEKMIKEGNNYRYPNGESLIDSYKRVAKEIDNIILENNSNLDTKTILICSHAGTIRN
                     IITHLISGSYKYHWNFKIDNASITVLEVDGGFAVIDKMNFTDFI"
     regulatory      12441..12620
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            12735..14249
                     /locus_tag="ACOIAF_001686"
     CDS             12735..14249
                     /locus_tag="ACOIAF_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HGZ2668700.1"
                     /translation="MGKKIMLQGTASNVGKSVLVAGLCRIFKQDGYTVAPFKSQNMAL
                     NSFITKEGLEMGRAQVFQAEAAKIEPIADMNPVLLKPSGNNKSQVIVRGKVVGEMPSS
                     KYHDYKLELVDVLKETFNSLNSRYDIVVMEGAGSNAEINLKDRDISNMGMAEIADAPV
                     IIVGDIDRGGVFASLAGTMLLLNEDEKRRVKGVIINKFRGKKELLQDGVKMLEDIIKV
                     PVIGVVPYTDIKIEDEDSVTTRFKKKMNKSDIHIEIIRTPHMSNFTDFNIFETQEDVS
                     IRYVDYGESLGNPDILIIPGTKSTIDDLTYIRKSGLESQIKNLHSQGKLIFGICGGYQ
                     MLGKKLKDPYHVESEIEEVDGIGLLDTETTFEKEKTTTQVEATIVECISEYMNNLKGK
                     KVKGYEIHMGITKRGNDVRSLDLITKKLDKEVNYTEGSVNKEGNVIGTYLHGIFDEIS
                     FTRAILNNIRKKKGLGELESKVNSFNEFKDKEYDKLADFLREHLDIEKIYEIMK"
     gene            14288..15664
                     /locus_tag="ACOIAF_001687"
     CDS             14288..15664
                     /locus_tag="ACOIAF_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HGZ2668701.1"
                     /translation="MKKILIAGTNSGVGKTTISLGIMQALVKRNMKVQPYKVGPDYID
                     PSYHTFITGRHSRNLDSYMLDDEKIKYIVNKSSKDADISVIEGVMGLYDGFGIDLDNC
                     TSSHTSKVLKSPVILVINGKSMAASSAAMVLGYKELDKDVNIKGVIVNNVKTNSHYQI
                     IKSSIEKYCNVEVLGYFPPNNKFSLESRHLGLVPSVEMKSLREKFYTLADEIEHYIDI
                     DRIIEISESDEFESDFDFQTLIENNKIKEHVNNKSIAIAYDKAFNFYYRENIELLEEL
                     GLEINYFSPLEDVSVPNADYIYIGGGFPEIFGKELEANESMRTSILEAHNNNIPIYAE
                     CGGLMYLGEKLLNQEEQEFNMVGIFSGISKMTSSLKRFGYCIGVAKENTLLSKKGESI
                     KGHEFHHSIFESDEKCVYSMRKERDGNIVEEWDGGYSKGNTLATYLHTHFYNNLNCIE
                     NFLKKGNE"
     gene            15665..16636
                     /gene="cbiB"
                     /locus_tag="ACOIAF_001688"
     CDS             15665..16636
                     /gene="cbiB"
                     /locus_tag="ACOIAF_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HGZ2668702.1"
                     /translation="MSILSIYIGYVTDLIVGDPYSFPHPVRFIGKLINFTQGIIRKVS
                     KNDRQLKFGGFILWFITVGITYLTTYAIVKLFSFNILLSIVVNSFIIYTTLATKCLKD
                     EALKIYNVLKTEDIEKSRIQLSYIVGRDTTNLSEPEIIRATVETVAENTVDGIIAPMF
                     YAFIGGAPLAMAYKAINTLDSTVGYKNKKYKDIGFASAKIDDIANYIPARISVILMTI
                     GSFFLNYNYRNCFKISIRDRKNHKSPNCAFSEGAVSGALGIQLGGTNVYFGEKVYKPT
                     IGDKLREIDKEDIIKTNKIMYASSFVSMLVFTLIYVLCRMILINLII"
     gene            16668..17738
                     /locus_tag="ACOIAF_001689"
     CDS             16668..17738
                     /locus_tag="ACOIAF_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HGZ2668703.1"
                     /translation="MKDLGHGANVDEMARLYGKNPKEIIDFSSNINPNVLPNLERYIL
                     KGLEECRNYPDINYTNLRENISKYIDINPDFIIPGNGATEVIYLLMKSIKKKLAIINP
                     TFSEYRRSAELNNLDIIDLELDLENNFKLNIDIIKENIKRFDSLFICNPNNPSGNVQD
                     LKELVHLLDKHNKVLIIDETFMEFVKDESKYSLVKYIESNKNIFIIKAVTKFFGMPGL
                     RLGYGLTSNTEIMNKIYEHKEPWTINSFADILSNFIFEDKEYIKNSKEYYIEEREYML
                     QELRNIRNIKVYDTDANFILIKIYKKTTKELKKDLFKQGNILVRDASNFIGLDDSFIR
                     VAIKSHEDNKILIENMKNLLGD"
     gene            17740..18627
                     /locus_tag="ACOIAF_001690"
     CDS             17740..18627
                     /locus_tag="ACOIAF_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HGZ2668704.1"
                     /translation="MKSYGICPASCGEFVQGIIDDEEYLCSYAIDMYSKVYIEEKLVD
                     INLGRYKSRLAIEKVFEKFNLPKKYTKNISLNINSKIPVGKGMASSTADIGATIKATL
                     SLIDKDLSSEEISKLAAEIEPTDSIFIDKNSIFNPLNGTVIKYLGNLTNAKVVILEPN
                     KVLDTMKIRLRQDYNKLKVENKEVIKKSFALLEEGLKKNHLSLVGEACTLSSLANENI
                     EKKEYLNEIIKISKKYGAYGVNIAHSGTVVGILIDKFMNDKKMIDALCESNINSVYNK
                     IYTQNIINGGIKGEIEWNT"
     gene            18615..19247
                     /locus_tag="ACOIAF_001691"
     CDS             18615..19247
                     /locus_tag="ACOIAF_001691"
                     /EC_number="5.4.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-8 methylmutase"
                     /protein_id="HGZ2668705.1"
                     /translation="MEYIKNPMKIEEKSFELIQEIIDEIRPDYKFKNNIEEKIIKRAI
                     HTTADFDYLDILKISEEAVDSIIDALKNNASIYTDTNMALSGINKRKLESLGCKYKCL
                     VADDETVKLAKEKGITRSMAAVEIAAKEEGRKIFVLGNAPTALYKVMEMKSEGRLDLD
                     AVIGVPVGFVGAQESKDEVEKTDIPYIISKGRKGGSNLAAAIVNAILYTM"
     gene            19256..20482
                     /gene="cbiD"
                     /locus_tag="ACOIAF_001692"
     CDS             19256..20482
                     /gene="cbiD"
                     /locus_tag="ACOIAF_001692"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase
                     CbiD"
                     /protein_id="HGZ2668706.1"
                     /translation="MEEYVYIDGKKYRRGYTTGSCATGASKAAVYMLITKNRINTINI
                     DTPKGIPLLLKVDNINISDTFVECSIKKDGGDDIDATHTMDIYARAEIVAKNDKNKGY
                     LTLKDIDSLSTNSECKSELYKFIRVYGGTGIGVVTKKGLSVDVGKPAINPTPLKMINH
                     EIRKLIGDNFESILGNDKVLKITIFAPQGETVAKKTFNPRLGIVGGISIIGTTGIVEP
                     MSDDGWKKSLSIELQMKKEQGLDKIILVPGNHGEQFIREKLNLDIKYVVRVSNFIGYM
                     IKEAQRIGYKKILMAGHIGKFIKVSAGIFNTHSKVADARSEILVANLALMGARYEFLN
                     KINQCVTTEEAVELINNSEYREVYNILSNKCRERVKQYLNEDSDDIDVEVIIFSMDKS
                     LLGKSDNTDDLVEVFI"
     gene            20479..21087
                     /locus_tag="ACOIAF_001693"
     CDS             20479..21087
                     /locus_tag="ACOIAF_001693"
                     /EC_number="2.1.1.289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-7 (C(5))-methyltransferase"
                     /protein_id="HGZ2668707.1"
                     /translation="MINIIGLGPGNLDYITKKGENLISTSDVLIGGKRNLESIKNFEG
                     EKIVLDSNLREIIEYINNNKEKQISIIASGDPLIYGIGRYLSKNIDNKMLNMVSGISS
                     LQYIFSKIYVDMNDVYITSSHGKVPDFDYILSHKKVCMVTDSKIGPKQISREIIDRNL
                     NKIIVVGENLSYDNEKITIAKPEEIIRMDNFDMNVVVILDEE"
     gene            21077..21652
                     /locus_tag="ACOIAF_001694"
     CDS             21077..21652
                     /locus_tag="ACOIAF_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating cobalt-precorrin-6B
                     (C(15))-methyltransferase"
                     /protein_id="HGZ2668708.1"
                     /translation="MRNSEFITGKVPITKEEVRAISISKLNLVNAKNLIDIGAGTGSV
                     SVEAAYNYPNLKVISIEKNDDAIELIEKNKKKFNLKNIEVIKGYAPINLGLNVKVESI
                     FLGGTGNNLEEIIEWSKKNLVTGGRLVANFIIIDTFNQTLKLLKKHGFKEIDVCVLNV
                     SKLEKLGKGEYFKPLNPIYIISCEKGENDDE"
     gene            21645..22397
                     /locus_tag="ACOIAF_001695"
     CDS             21645..22397
                     /locus_tag="ACOIAF_001695"
                     /EC_number="2.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-4 methyltransferase"
                     /protein_id="HGZ2668709.1"
                     /translation="MNKVHFVGAGPGDKELITLKGYKLLSNADVVIYAGSLVNPELLE
                     YCKEDCQIHNSAHMDLQEIIDVMREGIENNKSVVRLQTGDFSIYGSIREQVEDLNKLN
                     IDYDCTPGVSSFLGAASSLGVEYTVPEISQSVIITRMEGRTPVPEKESIQSYAKHQTS
                     MVIFLSVQEIEKVVSKLLEGGYPKDTPIAVIYKATWADEKIVKGTLSDIAVKVKENNI
                     NKTALIMVGRFLGEEYNNSKLYDKDFKHEYRG"
     gene            22381..23502
                     /gene="cbiG"
                     /locus_tag="ACOIAF_001696"
     CDS             22381..23502
                     /gene="cbiG"
                     /locus_tag="ACOIAF_001696"
                     /EC_number="3.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin 5A hydrolase"
                     /protein_id="HGZ2668710.1"
                     /translation="MNIEGNIAIICITENGKKLALKINSLVNNCVIYQVKNKKSNLVV
                     EETCVHTVQKKLSDFVGEIFSKFDYIVFIMATGIVVRSIAPYIISKFSDPAILVMDEK
                     GKNVISLLSGHIGGANSLTQYISSLIDANPVITTATDINQKASLDMIAKKLNAHIDNF
                     RESVKEVNSMLVNNKCVGLYVDEGYQVDTRGFKLIESKDELRDSFSNTYIEKLVVVSN
                     KINCIEDIENLNLDNKFEHCYSTQKIIKVIPRDIVIGIGCRRNTDSELMYNSLEEFLN
                     KQNIDIRAINTVGSIELKKDEKAIIELSKILKVPFKTFLIDEISKVDNLFDKSDFVKK
                     NVGVYSVAEPVAYLLSKGNLLVEKHKYKGITFSVGRIKI"
     gene            23499..24224
                     /gene="cobJ"
                     /locus_tag="ACOIAF_001697"
     CDS             23499..24224
                     /gene="cobJ"
                     /locus_tag="ACOIAF_001697"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030789 - precorrin-3B
                     C17-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HGZ2668711.1"
                     /translation="MIYVVGIGPGSKDTMTLEAIKVIEDSEAIVGYKTYIKLIEEFIQ
                     GKEIIQNGMRQEVDRCKQAVEEAKKGKKVAVVSSGDAGIYGMAGLILELISKEDEDIK
                     VKVIPGVTASIGAAAILGAPIMHDFCHISLSDLMTPWEVIEKRLRLAAEADFVICLYN
                     PRSKGRSEHLSKAFKIMGEFKGGDTPVGVVKDVGREKEEKFVCTFENMDFEKVDMTTM
                     VIIGNKSTYINGELMITPRGYTV"
     gene            24221..24973
                     /gene="cobK"
                     /locus_tag="ACOIAF_001698"
     CDS             24221..24973
                     /gene="cobK"
                     /locus_tag="ACOIAF_001698"
                     /EC_number="1.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016994 - precorrin-6A reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6A reductase"
                     /protein_id="HGZ2668712.1"
                     /translation="MILILGGTSDSLSICDKINELKNQPYILSVTTDYGRELAQQHAE
                     NVILGKLGKEDMLKFIKDNNIVKIIDATHPYAVEVSKTAITCAKLLNIDYIRFERKSL
                     IEEIDYDNKFIVDTIEEACEIANKIGTNIFIGTGSKNLNRFIEEISDKNLVVRVLPTS
                     EVILSCEKLGLSADNIVAMKGPFSQYINEETYKHYNIDLVITKESGIAGGFLEKVNAC
                     KTFNIPVIIIKRESINYENVFNDINKIKDILV"
     gene            24992..26470
                     /locus_tag="ACOIAF_001699"
     CDS             24992..26470
                     /locus_tag="ACOIAF_001699"
                     /EC_number="2.1.1.151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-factor II C(20)-methyltransferase"
                     /protein_id="HGZ2668713.1"
                     /translation="MKKALLVVSFGTSYHETREKTIDICEDKIKNSLKSHDFFRAYTS
                     NMIINKIKKRDGIEIDNPIQALDKIYEQGYDEVIIQTLHIICGEEFNKLKEQVDGYSS
                     KFKKIVLGRPLLTHIEDYQEAVEAIKHQIPHMESNEAVVFMGHGTLHESHSAYPALEY
                     MLRDNGINAYVGTVEGYPEIEHVIRRLKEGNIKTVNLMPFMLVAGDHATNDMAGDEED
                     SWKTILEENGFNVRVHLKGLGENSYIQDKFVRHAVKCAENAKFYGIGAGPGDGSLLTI
                     KAVNTLKKLDILYTPESKKGGDSLALSIVSEYLPESVEIKSRHFPMSFDGNEKSLAWD
                     NVAQEIISDVNDGKNVGFVTLGDPMIYSTYVYIMKRILGEVEVETIPGISSFSNIASN
                     QNFPLVMDKEALVVIPCTMEDDKIEYALQNYNSIVLMKVYKNFKEIIKKLEKYDLIEH
                     AILVSNSSQESENVFTDLKEAHLEDKISYFSTILVNKDNKIK"
     gene            26500..27405
                     /gene="hemC"
                     /locus_tag="ACOIAF_001700"
     CDS             26500..27405
                     /gene="hemC"
                     /locus_tag="ACOIAF_001700"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HGZ2668714.1"
                     /translation="MNIVVGTRGSNLALIQTEWVINELKKKYPEISFEIKIIKTKGDL
                     IQNVSLDKIGDKGLFVKEIEQQLLDGKIDIAVHSMKDMPSYLADGLKFAHTPKREDPR
                     DVLILREGYKNLDDLPHGAVIGTGSKRRKFQLLKQRPDLNIVQVRGNVETRIRKIKDE
                     NMHGIVLAASGIIRANLQDKISSYLPVDVVIPAPAQGALAIEIRSNDSTIEGIVNSLK
                     DENTEIQILAERGFLDGVNGSCHIPMAAYCEIIQDKIHLTGLYGDSEGKKVVIKSIDG
                     DISSPRELGLKLAKLVLKEYENYEG"
     gene            27395..28894
                     /gene="cobA"
                     /locus_tag="ACOIAF_001701"
     CDS             27395..28894
                     /gene="cobA"
                     /locus_tag="ACOIAF_001701"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HGZ2668715.1"
                     /translation="MKGKVYLVGAGPGDYKLMTLKGLECIRKSDVIVYDRLANSSYLR
                     EAKPDCELIYVGKASSNHILPQEDINRIIAEKAKEGKIVTRLKGGDPYVFGRGGEEAE
                     TLVDEGIEFEVVPGITSAIGGLCYAGIPITHRDHASSFHVITGHLKGDDSGELNWNAL
                     ANNKGTLVFLMGISNLEKISENLMKEGKDKDTPVALISWATRYNQRVVTSTLENVYET
                     AIKEEVKPPTLIVVGSVVGLREKLNFFESKPLFGKSIAVTRSRNQNSVLVEKIMDLGG
                     NPIEIPTIKVEKIQNNINLENEIKNINKYNYLILTSKNAVEIFFEKIYEMNFDLRILS
                     NLKVCAIGSATSNELKKRGIIADIVPKKFVAESLYEELAPILNDDDNILIPRAENARD
                     YLVDKLKAICEVTEVHIYRTVIDEEKKGEILDILNSNDIDYITFTSSSTVNNLVEIIG
                     KENIKILEKIKTLSIGPITTETINSLGIKLYKESATSTIDSLVDLIIKN"
     gene            28911..29876
                     /gene="hemB"
                     /locus_tag="ACOIAF_001702"
     CDS             28911..29876
                     /gene="hemB"
                     /locus_tag="ACOIAF_001702"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HGZ2668716.1"
                     /translation="MLRRPRRLRASKAIRNLVRETKVNVEDLIYPLFVVEGENIKEEI
                     KSLPDVYHLSIDMLEDEIKEISELGIEHIMLFGVPDEKDECASQAFNDNGIVQKAIRK
                     VKEINSDINVITDVCMCQYTSHGHCGILTDTGYVDNDKSLEYLAKISVSHAKAGADMV
                     APSDMMDGRIQAIREALDENGLENVGIMAYSVKYASNYYGPFREAAHSAPSFGDRKTY
                     QMDPANSNEAIIETELDILEGADILMVKPALSYLDIIRRVKDNFDLPLAAYNVSGEYS
                     MLKFAVKEGLLNEDVILESITSIKRAGADIIITYFAKDIAKLLKK"
     gene            30029..30607
                     /locus_tag="ACOIAF_001703"
     CDS             30029..30607
                     /locus_tag="ACOIAF_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HGZ2668717.1"
                     /translation="MLYPINLKLDELDVVIIGGGEVAYRKCKNFLEFNKNVTIVSKQI
                     LNKFYDLKGNIKIIKDDYKEIYIKDASVIIAATNNRELNMEIGLYCRAKNKLVNVVDN
                     IEISNFTVPSYIKRGDLLISVSTGGKSPSLSSKIKKEIEERYTEDYEEYLNVLGDIRK
                     KVIKKYEDKSKRKDVLNMLVTLDLEELKKFDI"
BASE COUNT        12427 a         3062 c         5676 g         9532 t
ORIGIN      
        1 tggaattaaa atatactagg atataaaatt cttaaataaa aagaaaaggt tgtttttata
       61 aaaaatattt tttataccac tttaaaaaac acaataataa cactgaggga ggtggggata
      121 tgagagaacg cattattctt gaaacattaa aaaatagtag tggttatcta aacatagatt
      181 attttgcaga taagttgggg gtatctactc gaactatcag aaatgaaata aaaaagatag
      241 aaactataaa agaatgtaat ggttttaaat tagaatataa gtctaaatta ggatatattc
      301 taaatattaa agaccaagat aaattcgagc attatctaag aaacttaccc tcagatttag
      361 ttgaaaatcc agaacaacgg ttggagtcta taatagtaga gcttttagta aatgaaggtt
      421 ataaaactat agaacagtta agtaaaaaat ttctagtaag ttcatcccaa ataaaaaatg
      481 atttaaaaaa gatagatgaa aagataaagg atacagaatt aaaactagag cgaaaggctc
      541 attatggaat taaaatagaa ggttctataa aagaaattca aagtatattg gtggatagtt
      601 attttagagg gaatagaaat ataacagagt acaggaataa gtttatagat aatttaaaat
      661 tagctaatat tagaagtaca ataaaaaatg tacttaatga acatgattta gaagcaaatt
      721 taacagaact tgaggaaata ttagcacaaa ttattatatt gtatataaga gttagtatga
      781 gagtagcggg aaatgctagc gatttaaaat tatatacaga agatttaatt atagataaat
      841 tattagataa aatttttaga gataaaaaat ataaccttaa ttatgatgaa aagtattatt
      901 taaaggagct tataaaacta aagactaaag ataagaaagc cactataaag aatattgata
      961 agaataaact tcaagatatt atgttcgaat tttttagaga gatagataaa aaatataact
     1021 ctaatttttt agaagataaa gagttcttta acttatttta cttacatatt gcatgtttaa
     1081 tagaacgtat aaagaagaac cacaagataa taaatccttt ttcagttaaa ataagtcaac
     1141 agtatccaac tgtttttaat ttatctattc aattttcaaa aatcatagaa aatgagtatc
     1201 atataaaaat aagtcaagat gaaatagggt ttatagctac tcacattgct gttccttttg
     1261 aaaagagaga agaagctaat tttaataaaa aatataaaat agcaataata tgctcttctg
     1321 gtggaggtag tgcatttttg ataaatttaa gacttaaaga aatttttcca aatgctgaga
     1381 taagaaattt ttcattacta gaagaaaaag ctgtaataga atttgcacca gatttgattt
     1441 tttcaataac aaatctttta tttgaaataa atgctccagt tatacttata aatgaaattt
     1501 tagacgaatt ggattattta gatattaaag aaagtgttag atttgcagac catataggaa
     1561 atatatcaag cccaaaacaa tatatattag gtctatttga taagaatcac tttaggtgta
     1621 taaaagataa agctaagtat aaggatatcc ttgatagtat gtcaaaatgt atagttgacg
     1681 agggcgcttg ttctccaacc tatcccaaag atgtatggga aagagaaagc tatttgagta
     1741 ctatttatac aaatggggtg gctatacctc atccaatcga gatgacagga aacaaaaata
     1801 taatatcagt agctttgata caaactgata ttatttatga aaatagggtg cctaaaatta
     1861 ttttcatgat ttcattaata aagggaaatt tagaacttca taagcaaata tctaaatatt
     1921 taagtaagat tatggcaaat aaggatatgg tagacatgct aaataagtca caatcttatg
     1981 aagagtttat gtataagtta aaaatctatc tgggaggtta gacatgaata tagagttttt
     2041 taagaggttg tcggaagcag atggtatagc atctaatgaa gaagaggtta gacatgtttt
     2101 attggaagaa ctaaaagctt atagtgataa aattatctgt gatgggctag ggagtatcat
     2161 cttttctaaa ataaaagatg agagtgcacc aaacgtaatg atttgtgctc atatggatga
     2221 agttggattt atggtgagaa gtatagacaa acttggtatg attcacttaa taacaatagg
     2281 aggagtaaaa cctcttgcac agtttgtaca aaaagttaga attacaacaa aagaagggaa
     2341 gaaaatccct gctgtcataa atgctacata taataatggt aaggctgaaa atatatatgc
     2401 agatattgga gcatatacag aagaagatgt atataaccta gggattaatg ttggggatat
     2461 ggtaacatat acaacaagct ttgaggaatt tactttacca gaccgattag tgggaaaggc
     2521 ttttgatgat aggataggat gttttgtgat gggagaagtt ttaaaagaac ttaggaaaga
     2581 aaatctaaat tgtaatattc actttgcggc tacaagtagt gaagaggttg gaataagggg
     2641 agcaaagaca tcaacacagt taataaatcc agatatagta tttgtaatag atgtagcatg
     2701 tgctaaaaat gagtttgtga gagactatac aaatcagaaa caaattgata aaggtgtaat
     2761 gctaatgcat agagatagga ctctagtccc tagtagaaaa atgatagatt atgtaatgga
     2821 aattgctgat aaaaataata taccacttca acatgatatg tttgaatcag gtgggactga
     2881 tggaggagaa gcacatctcg taaatgaagg gaaaccttgt gtagtaactt gtgtaccagt
     2941 tagatatggt cattgtgcat tttcaatagt aagtaataaa gatttagaga acattataag
     3001 attgtataca cagttaattt taaattttga tgaagaacaa tataaacata taagaaattt
     3061 tatctaaagg agaataagaa aatgtcaaca gaagtacaaa taatggaact tatatcaact
     3121 gcaggagaga gtaaatcgaa agcttttgag gctttaaaaa aagtgaagtc aaaagatttt
     3181 aaaggagcaa aattgttaat agaggaggct cgtaaaatag atattgaagc tcacaggata
     3241 caaacagaat taataactca agatttaaat gaagatgttg aaagtccagc aatgagtctt
     3301 ttaatggtac atgctcaaga ccactatatg acttctcagc tagcaaaaga cttgatagag
     3361 gctttgatat attgtcttga taagtaattg taatatagaa atagattatt tatgtaaaaa
     3421 ttcttttaaa agtttagatg cttttaagag ataataaaaa tacaaacttt aggaggaatt
     3481 attatgaaaa tattattatg ttgtgcaggt ggattttcta ctaatatgtt aatgcaaaat
     3541 atgaaaaagg tggtaaaaga gagtgctaaa ctcaatgaaa aagattttga gtttacagct
     3601 ataccagcag attcaataga aagtgaagtt gatgattggg atatagtact tataggtcct
     3661 caaatagctc acaaaataga cttcttaaaa ggaatattag agccaaaaaa taagcctttt
     3721 gcagtaatag ataaagatgt atatggaaaa atggatggag ctacagttat gaagttggca
     3781 ttaataactt ataagaaaca tcaattagct caaggataat aaaaataatg agggaaaagg
     3841 gagattagat atgttttcaa aatttgaagc atttatgaat agagtattta tgcctttagc
     3901 tcataaggtt gacaatcaac gtcaccttgg ttctataaag gctggtatgg ttgctatgac
     3961 accattcaca atattaggaa gtatatttgc aatacttcca gcacttccaa atatgcttgg
     4021 agaaaataat ccaataagca aattcatact taacaattca gcaatattgg atttaccagt
     4081 aagtctttcc ataggtttaa taggattata tgcttgtatg tgtattgctt ttaatctggg
     4141 gaactactat aaattatata taccaggttg tgttgcacta tcaacttttt cttttttatt
     4201 cctagtagcc tcatttacac cagatggagc tttagaaata ggaaatttag gagctaaagg
     4261 gctatttaca ggtatgatag cagggcttgg ttctgtggag ttatataatt tttgtaataa
     4321 gaagaatatc acaataaaaa tgccagaaaa tgtacctgat tttgtatcta ggtcttttga
     4381 attaatacct actactataa tagtttgtgg aacttttata gttgctcgtt ttatttcatt
     4441 aaaagtattt ggagaattac ctccacagat acttactcaa tttttatctc cattagtagg
     4501 aagtatggat aacccatggt cagttcttgc acttaacttt gctatatgta ctatattctt
     4561 ttttggaata cattcatcag tatttagtcc tataactaga cctataatga ttacatttat
     4621 agctgaaaac atagcagcta tgcaagctgg agaaacaata cctcatttct ttacagcagg
     4681 agcttcaagt gcattctttg gatttacagg atgtggaatt agtataggtt gtgtaattgc
     4741 atgtatgtta tcaaaaaatg cttcctataa gaagattgga cgtgtatcat tattccctgc
     4801 attatttggg ataaatgaac caatattatt tggagcaccg ataatattaa atccaataat
     4861 gtttatacca catgtatttg gtggagctat aataggtaca ctaccaatgt tttttatgca
     4921 ctggggatgg ttagcaaagc cagtatttga ccctccatat gtaggggtat tcttagaagg
     4981 atttttaact aatggagatt ggcattctat attagcaaat gcattgcaat tagtattagc
     5041 agttttatta tattggccat tctttaaaat aatggaacgt caacaaaata ataaaaaaca
     5101 aaaagaagaa actaagaata tattctctaa agaagaagaa gacttattgg atgatttagg
     5161 attggatttc taatattttg ttatgaataa tacaatggag agattagtat ccttaatcta
     5221 taaaagaggt gttgacgctc ttttgattaa agcaaaaaat actaaaagat atataggagc
     5281 tttatcaggt agtggtgtat atgtgcttgt tactaaggat aaaaattatc aaattctaga
     5341 tggcagatat attgatgaag cagataaaaa aacgactggt ttcataaaaa ggattgtatc
     5401 tcaaggttct tatataccaa ctataattga gttattaaaa gaattaaata taaaaaagtt
     5461 agcaatagaa aaccaagcta tgagtataca agagtatctt tcactaaaaa atgaaggttt
     5521 tgaaatcaat ttaataacaa atgaattatg gaagctaagg gctataaaat ctaaagaaga
     5581 aattgaactt attaaaaagg cttgtgagat tacagatgaa gtatttgatg aggttatttc
     5641 tgaaattaaa aaagatatga ctgagctaga agtatcagct ctaattcagt atcatgcatt
     5701 aaaaaaaggt gcaagtggaa tgtcttttga aacaatagtg gtatcaggag aaagaggtgc
     5761 aatgccacat ggaaaaccta caaacaaaaa attgaaagtg aatgaagcta taacaataga
     5821 ttttggtgtt gtttatcaag ggtatcaatc agacatgact cgtacaatat ctataggtaa
     5881 acctcctaaa attattaaag agatatatga tgtagtattg gaagcacaat tatctgcaat
     5941 agagtcaata aaggaaggaa ctagggcaag tgatgttgac aaagtggcta gaaaaattat
     6001 agataaacat ggatttggcg aatattttaa tcatggactg ggtcatggta taggtcttgg
     6061 agatggtgaa gtccccactt taaattcaaa tagtgaagat atattagtgg aaggaatggt
     6121 aatgtcttgt gagccaggta tttatattcc aaatgtaggg ggagttagaa tagaagatga
     6181 cattgttata atagatggaa aaggtgttcc actcaataag acatccaaag agtttataat
     6241 attaggagag taaaattatg aaatataatt ttaataaagt attaaatcgt aaagaaggaa
     6301 attgtagaaa atggagcaac catgtgatta aagaaaaatt tggcttaaat gaagatgcaa
     6361 ttcctatgga tttagctgat atcgattttg agtgtgctcc tgctataaaa gaatctatct
     6421 taaaaagagc atcagtagga gattatagtt atacttttat aggagatgac ttctatgatg
     6481 cgattataaa ttggaataaa agaagatttg atgtatatat agaaaaggat tggataaaat
     6541 taacttttgg tactgtttca acccttcatt atatagtcca agcatatact aaagaaggag
     6601 agggtgtatt gataaataca cctgcttatg acccttttgc agaggctgta gtaaataata
     6661 atagaaagtt gtgttgtagc tcattaaaat tagataacaa tagatattat ttagatttag
     6721 aggatatgga aaatcaaatg aaatacgaga atataagatt gcttatattc tgtagtccac
     6781 aaaatccatc aggtcgtata tggacaaaag aagaactata tcaagtgtca gagttatgtt
     6841 taaagtataa tgtaattttg gtaagtgatg aaattcatag agatataata tttaaagatt
     6901 atagatttgt atctttatgg aatgctcatc cggaaattta taaaaatagt ataatctgtg
     6961 tatctcctaa taaaggcttc aacttaggtg gattaaaaac atcatatgta ttaataagag
     7021 atgtaaaaat tagggagact ttattagaaa ggctaaaaag taattcaata acttctccaa
     7081 atgtatttgc aataccagca atagttgcag catataatga aagcgaagaa tggctagatg
     7141 ctatgactag ttatgtagag gagaattttg aaatagttta tgatttcttt gaaactaata
     7201 taccaaaagc taaagtgatg aagtcagact cttctttttt agcctggata gatgtaagag
     7261 aagtatttag aaatgaagaa gaatctaaag aattttttag acatgcaaac ttaactatgg
     7321 tagttggaag ttattttgtg aaagatggag atggatttat tcgtattaat gtggggtgtc
     7381 ctagagaaac attaaatgag gctctaaata gaatcaaaaa aacgtatata agtatgtatt
     7441 gttaaatcat aaaaaaagta tctatggttt atataaaaac acattattta gttatcaaat
     7501 attactggaa ttttgtattc cagtaatatt ttttgattaa ggaatattat tgaggaatat
     7561 taaataatat tatttcatac ccttatatta acttatataa aaatatttta ttattttggg
     7621 aacgggttag ctaaccttta tctagtgtat atttatattg taagaagcag taaaaacttt
     7681 tcctttacta agtttattga taaacttctt atctttataa attttgttag aatttatact
     7741 atcagcaaga ccataccaag gttctataca taaaaaagga gcagtagaat tagtttcact
     7801 ataataagga gtccaaatac caactagagg gaaatcttcc atagatatag ttacatattt
     7861 actaccatca ttattacata tagacacttc atctatgttg gtgtatatca aagcatcatt
     7921 cttgaatact tctggattta actgtaagtt ttttaatttt ccaataggta ttatatcatc
     7981 tgtaaaagaa ccatttaaat tgattttttc tacatcattt ttacttttaa actcaaggta
     8041 gtgtttagaa aaatcatttt gttcgtaaaa aggaaggttg aatgcaggat gtccacctat
     8101 agaaaagaat atatctttag aatcagtatt ttttacaatc cattctacct caatactatt
     8161 ctcatttaat gtatagttta tgattagctc aaaagaatat ggatacttct ttaaagtaga
     8221 ttcattatct gttaatttat aagttacaaa tgtatctcct ttatctacaa tctcaaagtc
     8281 catatctcta gcaaacccat gttgagtcat gctatataaa ttttcttcta ttattgtttc
     8341 attatcaaaa agtttaccta ctatagggaa caatataggt gattgtctac cccaatattt
     8401 tttattacca tcccaaagaa tgtttttgtc gtatttttta gaaaatattt tagttagttc
     8461 agcaccaaaa ctgcttgatt cgataattaa attttcattt tgtaatatat tcatttaaaa
     8521 tctcctttat tatattttat gagtatatta tactataaag tataaagatt attaaatttt
     8581 atttgatatt atatttgaaa ggtagatttt agtatatttt gaaaattttg ttttgaaaat
     8641 ttgggattat ttaagaaatt ttgtattata aatagaagga aatagataaa ttgtatagaa
     8701 taaaatatat taagataagt taataatgtt aaatgggaag tgagtttatg gaaaaaccaa
     8761 atacaaaagt tgttaacttt caagagtata aagacagaaa aaaccaaaga gaagtaaata
     8821 gagacgaact tttaaagctt ataaaaaaaa ttgtgacttc aaaataggga gttgttaaac
     8881 tccctatttt aatgggtttc ataataatta agacattcta agtccatcta tataatcttt
     8941 gaattgtgag aaatctgatt cactacttaa ttctatggtt tctgaacctt ccgtataaaa
     9001 attcttaaga tagatagtat aattattatc aacacaatca tgataaatac atctatatcc
     9061 atcttcatac aaacttttta acttagaaaa atcatccaca atatcatctc cttaaagttc
     9121 ttttaaatat atttttacaa aaagcatcta attgtatgca aattgacaat gtaaaataaa
     9181 ggaaatgaac ataaaatgta gaataatttt tatatgtatg taaaaataaa aagaaaaagt
     9241 tgaaattttt agaaaataag agtatttttt atctaaaaag agagtttaaa aaataaaaaa
     9301 atgttgggag gataaaaaat gagtttatta gaaagtatat caaaaaacat atattcccta
     9361 gataactctt ctattgaaaa aacaaagcag agattggata ggcttataca tccaactgga
     9421 agcttaggaa agatagaaga tatttgcatg caattagcag gaatatttgg caatgaaaat
     9481 tttgacacaa gtaaaaaggt tataattgct tttgcgggag accatggtgt atatgaagaa
     9541 ggcgtagcac ctgaccctca aaatataaca aaattacagt ttccaaattt ttcgaaaggt
     9601 ctatgtggag ttggagtaat aagtaaattt gtaggggcag atgttgtagc tgtagatgta
     9661 ggaataaact gtgatgaaaa actggatggg gtattagact acaagataag aaaagggaca
     9721 tcaaatatgg caaaaggtcc agctatgagt aaacaagaag caattagatg tttagagata
     9781 ggtattgaaa tagcagagca gtgtatagaa aaagattata aggttattgg tattggggag
     9841 atgggtatag caaatactac tccaagtaca gcaataatat ctgtaatttc agggtgtgac
     9901 ccgttagaag ttactggtat aggtgcaggt cttaagaaag aaagactaaa gcataaggct
     9961 gaagtaatta gaaaggctat tgaaattaat aatccaaatc caactgatgg agtagatatt
    10021 ttatctaaag taggagggtt tgaaataggc tcaatggcag gtgttatatt aggttgtagt
    10081 gctaatagga ttccagtagt aattgatggg tttatttctt atgcagcagc attaattgca
    10141 tataagataa atccaaaaac tagagaatat atgatagcat ctcatttatc agcagaatca
    10201 ggaactaaaa gagcattaga catattaaaa ttagaccctc tattaaatat ggatatgaga
    10261 ttaggtgagg gaagtggagc tgctttagca tttgatataa tagaagcttc aaactatact
    10321 tacaaaaata tggcaacttt tgatgaaatt gacatgggaa gataaatata aaaatttaat
    10381 atgtaggagt gttttgatga gtaaaattat tctagtaaca ggaggagcta ggtcagggaa
    10441 gagtaacttt gcagaaaaac tatgttttga cagaaataat aatacagctt atatagcaac
    10501 ctctattcca tttgatgatg aaatgaaaga tagagtaaaa aaacatcaag agaatagacc
    10561 tagaaactgg aatacttatg aaatatatga agatatatat tccataatta aggagatata
    10621 taaagaacat gaaactgtta tactagattg tgtaacatta ttagtaaata atctaatgtt
    10681 tgaacacaat ctaaatatag aagaatctag tcaagaagaa ataaacaaat tagaacaata
    10741 cataaaagaa caagttgtca aacttattga agaaatagaa aaaacaaatc tatactttgt
    10801 atttgtaacg aatgagttag gtatgggaat tgttcctggg aataaactta gtagaattta
    10861 tgctgatatt gtaggtcgta taaatcaata tatagcttcg aaaagtaatg aagtttattt
    10921 tgtagttagt ggcatagcta tgaagataaa ggagtagtat gcaattatga aaagatttat
    10981 tttaatacta cagtttttga caaggatacc tataaaatta aatgtaggat ttgatgatga
    11041 attttataag tcaatagtat attttccact tgtaggtttt gtcataggta tattatccta
    11101 tttaatagga tggatttcta tgcttttatt tgaaccattt atagcttcta taataataac
    11161 tctagctggt gtcttaatta ctggaggact tcatatagat ggattaggag atacatttga
    11221 tgcaatatat agttataggg ataaagaaaa aatgcttgaa ataatgaaag attcaagatt
    11281 aggtacaaat tcacttctag caattatgtt tgtactttta ttaaaggttg gattcgttta
    11341 cgatataata agtaataatt cattgtgggt tataattttt atgccaatga ttgcaaggtt
    11401 aggagtaatg ttattgacat ataaaactgt gacaccaaga gaaaaaggaa tgggaaattt
    11461 atttatagga aaattaacta caagtatgtt aataactgct ataatataca cattattgat
    11521 agttgctctt attactaaat ttatattttt attacctaat atagtgttga ttaaagtatt
    11581 aggttcgatt atagtggttt ttgtatttat aatattattc aaaaaacata tttataaaaa
    11641 aattgacgga gtaacaggag atatattagg ctgtggaata gaactttcag agcttgtata
    11701 tttaatttac atatatttgt taatttttat gtttttttaa agtaaaaagt ggtgataata
    11761 tgataagatt aatattaata agacatgcac tgacaaatga taataaaaaa gaaagattat
    11821 caggccatat aaatagttgt attagtgaag agggcaaact acaaataaat aaaattacca
    11881 gatatctgag taatgaaaac atagataagg tttatacaac accatctaca cgtactaaag
    11941 atacagtaga aaaaatatca aaattgaaat tattagaaat agaagaaaaa gaagctttaa
    12001 gagaaattag ttttggagac tttgaaggaa gaacttttga agaaattaaa atcaaatatc
    12061 caaatgagtt tgaaaaaatg ataaaagaag gtaacaacta tagatatcca aatggagaaa
    12121 gtttgataga ctcttataaa agagtggcga aagaaattga taatataatt ttagaaaata
    12181 atagtaattt agatacaaag acaatactta tatgttctca tgcaggaaca ataagaaata
    12241 taataacaca tttgatttct ggtagttata agtatcactg gaactttaaa attgataatg
    12301 catctattac agttttagaa gttgatggtg gatttgctgt aatagataaa atgaatttta
    12361 cagacttcat ataatttaat caagaccaaa cattacaatc tgtgtttgta atatatctgt
    12421 aaaattagca atgaaaatag aatattagat taaaattagg ttcttaatta gatgttaaaa
    12481 gggaaaaagg ttagatgcct ttgcagcccc cgctactgtg aaaccaacga aactataaat
    12541 accactgtca atttgatggg aaggttatag aagtaggatg aagttaagcc aggatacctg
    12601 cctaatttaa tttaaaacaa gaggtcttcg gggtgaaaga tttttaatta atatatataa
    12661 aagtcctatc tttagaagta ttaggacttt ttttaatttg ttttttagta aattttaaca
    12721 aggagggatg gaaagtgggg aaaaaaataa tgctacaagg aacagcttca aatgtaggta
    12781 aaagtgtctt agtagcaggt ttgtgtagaa tatttaagca agatggttat actgttgctc
    12841 cttttaagtc acaaaatatg gctttaaatt cttttataac taaagaagga ttagaaatgg
    12901 gaagagcaca agtatttcaa gcagaggctg cgaaaataga gccaatagct gatatgaatc
    12961 cagttttatt aaaaccttct ggaaataata aaagtcaagt tattgtaaga ggtaaagttg
    13021 taggagaaat gccttcttct aagtatcatg attataaatt ggagttagta gatgtgttga
    13081 aggaaacatt taatagctta aattcaaggt atgatattgt agttatggaa ggggcaggaa
    13141 gtaatgcaga gataaactta aaagatagag atatctctaa tatgggtatg gcagaaatag
    13201 ctgatgcacc tgtaataata gtcggagata ttgatagagg aggcgtattt gcctcattag
    13261 caggaacaat gcttttatta aatgaagatg agaaaagaag agtaaaaggt gtaataatca
    13321 acaaattcag agggaaaaaa gagcttttac aagatggtgt aaagatgcta gaagacatca
    13381 taaaagtacc tgttatagga gtagtacctt atacagatat aaaaatagaa gatgaagaca
    13441 gtgtaactac gagatttaag aaaaaaatga ataaaagtga tatacacata gaaataatta
    13501 gaaccccaca tatgtcaaat tttacagatt ttaatatatt tgagacacaa gaagatgtaa
    13561 gtattagata tgtagactat ggagaatctt taggaaatcc agatatatta ataatacctg
    13621 gaactaaaag tactatagac gatttaacat atataagaaa aagtggtctt gaatcgcaaa
    13681 tcaaaaattt acatagtcaa ggtaaattga tatttgggat atgtggtgga taccaaatgt
    13741 taggtaaaaa attaaaagac ccttatcatg ttgaaagtga aattgaagaa gttgatggaa
    13801 taggtctttt agatacagaa acaacgtttg agaaagaaaa gactacaaca caagtagaag
    13861 ctactattgt tgaatgtatc agtgaataca tgaataacct taagggaaaa aaagtaaaag
    13921 gttatgaaat tcatatggga attacaaagc gaggtaatga tgtaagaagt ttagatttaa
    13981 taactaaaaa acttgataaa gaagtgaatt atactgaggg aagtgtaaat aaagagggga
    14041 atgtaattgg gacttatctt catggaatat ttgatgaaat aagctttaca agagcaattt
    14101 taaacaatat aagaaagaaa aaaggtttag gagaattaga aagcaaagta aactctttta
    14161 atgaatttaa ggataaagag tatgataagt tggctgattt tttaagagaa catttagata
    14221 tagagaaaat atatgagatt atgaaataat gatgactttt atttataatt taggtaggag
    14281 gtgtattatg aaaaaaattt taattgcggg tactaatagt ggagtaggta aaacaacaat
    14341 atcattagga ataatgcaag ctttggttaa gagaaatatg aaagtacagc catataaagt
    14401 agggccagac tatatagacc cttcatatca cacttttata actggaagac attctagaaa
    14461 tttagattct tatatgttag atgatgaaaa aataaagtat atagtaaata agtcctcaaa
    14521 agatgcagat atttcagtaa tagaaggtgt tatgggctta tatgatggtt ttggaataga
    14581 tttagataat tgtacaagtt cacatacttc aaaggtatta aagtcacctg ttatattagt
    14641 tataaatggt aaatctatgg cagcatctag tgctgctatg gtacttggat ataaggaact
    14701 agataaagat gtaaatataa agggtgttat agtaaataat gtaaagacta atagccatta
    14761 tcaaataatt aagagttcta tagaaaaata ttgtaatgtt gaagtattgg ggtattttcc
    14821 tccaaataac aaattctctt tagaatctcg acatctagga ttagttccta gtgtagaaat
    14881 gaaatcacta agggaaaaat tttatacctt agcagatgaa attgaacact atatagatat
    14941 tgacagaata attgaaatat cagaaagcga tgaatttgag agtgattttg attttcaaac
    15001 tttgatagaa aataataaaa taaaagaaca tgttaataat aaatcaatag ctatagcata
    15061 tgataaagca ttcaattttt attatagaga aaatatagag cttttagagg aacttggttt
    15121 agaaataaac tattttagcc ctcttgaaga tgtatcagtg ccaaatgcag attatattta
    15181 tattggagga ggttttccag aaatatttgg aaaagaatta gaagcaaatg agtcaatgag
    15241 aacttccata ttagaagcac ataacaataa tatacctatt tatgcagaat gtggaggact
    15301 gatgtaccta ggtgagaaac tattgaatca agaagaacaa gaatttaata tggttggaat
    15361 atttagtgga attagtaaga tgacatcttc tttaaagaga tttgggtact gtattggtgt
    15421 agccaaggaa aatactcttt tatctaaaaa aggggaatct attaaagggc atgagtttca
    15481 tcattcaata tttgaaagtg atgaaaaatg tgtatattca atgagaaaag aaagagatgg
    15541 aaatatagta gaagagtggg atggtggtta tagtaaagga aacactttgg ctacatatct
    15601 tcatactcat ttttataata acttaaattg tatagaaaat tttttgaaaa aaggaaatga
    15661 ataaatgagt attttatcta tttatatagg atatgtgaca gacttaattg taggagaccc
    15721 atattctttt cctcatccag tgagatttat tggtaaactt ataaatttta ctcaaggtat
    15781 aataagaaag gtctctaaaa atgataggca gttaaaattt gggggattta tattatggtt
    15841 tataaccgtt ggaataacat atttaacaac ttatgctata gtgaagttat ttagttttaa
    15901 tatattattg agtattgtag tgaatagttt tataatttat acaacactag ccacaaaatg
    15961 tttaaaagac gaagcattaa aaatttataa tgtattaaaa actgaagata tagaaaaatc
    16021 taggattcag ctatcttata tagtaggtag ggatactact aatttaagtg aacctgaaat
    16081 aataagagca actgtagaaa ctgttgcaga aaatactgta gatggtataa ttgcgcctat
    16141 gttttatgca tttattggag gagcaccact tgctatggcc tataaagcta tcaatacatt
    16201 ggactcaaca gttggatata aaaataaaaa gtataaagat attggttttg catctgctaa
    16261 gattgatgat atagccaatt atataccagc tagaatatct gtcattctta tgacaattgg
    16321 aagtttcttt ttaaattata actataggaa ctgttttaaa atctctatta gagatagaaa
    16381 gaatcataaa agtccaaatt gtgctttttc agaaggtgca gtatctggtg ctctaggaat
    16441 acaactagga ggtaccaacg tttattttgg ggaaaaagta tataagccaa caatagggga
    16501 taagcttagg gagatagata aagaagatat aattaaaact aataaaatta tgtatgcatc
    16561 ttcatttgta tctatgttgg tatttacact aatatatgtg ttatgtagga tgattttaat
    16621 taacttaatt atataaatta ttgtttgtta acttaagggg gacatagatg aaggatttag
    16681 ggcatggtgc caatgtagat gaaatggcaa gattatatgg gaaaaaccca aaagaaataa
    16741 ttgattttag ttcaaacata aatccaaatg tattaccaaa tttagagaga tacattttaa
    16801 aaggattaga agagtgtagg aattatccag atataaatta tacaaacttg agagaaaata
    16861 tatcaaaata tatagatata aatccagact ttataatacc tgggaatggt gcaacagaag
    16921 tgatttattt gcttatgaag agtattaaaa aaaaattggc tataataaat ccgacttttt
    16981 cagagtatag aagaagtgct gagttaaata atttagatat tatagattta gagttagatt
    17041 tagaaaataa ctttaaactt aatatagata taataaaaga aaatataaaa agatttgata
    17101 gtttatttat atgtaatcct aataatccta gtggtaatgt ccaagattta aaggaacttg
    17161 ttcatttact agataaacat aataaggtac taataattga tgaaactttc atggaatttg
    17221 ttaaagatga aagtaaatat agtttggtta aatatataga atcaaataaa aatatattta
    17281 taattaaagc tgtgactaag ttttttggta tgcctggttt aaggctagga tatggtctta
    17341 caagcaacac tgaaattatg aacaaaattt atgaacacaa agagccatgg actatcaatt
    17401 cttttgcaga tatactatcc aattttattt ttgaagataa agagtatatt aaaaatagta
    17461 aagaatatta catagaagaa agagaatata tgttacaaga gttgagaaac ataagaaata
    17521 taaaggtcta tgatacagat gctaatttta tcttgataaa aatatataaa aaaacaacta
    17581 aagaattgaa aaaagatttg tttaagcaag gaaacatatt agttagagat gcgtctaatt
    17641 ttataggatt agatgatagt tttataagag tggctataaa atctcatgaa gataacaaaa
    17701 ttcttataga aaatatgaaa aatttattgg gtgattaaaa tgaaatctta tggaatatgc
    17761 ccagcatctt gtggagaatt tgtacaaggt ataattgatg atgaagaata tttatgttca
    17821 tatgcaatag atatgtattc taaagtgtat attgaagaaa aattagtaga tataaattta
    17881 gggagatata agtcaaggtt agcaattgaa aaggtatttg aaaaatttaa tttacctaaa
    17941 aaatatacta aaaatatttc tttaaatata aatagtaaaa tacctgttgg aaaaggaatg
    18001 gcaagttcaa ctgctgatat aggagctact ataaaggcaa cattatcatt gatagataaa
    18061 gatttaagta gcgaggagat ttctaaatta gcagcagaaa tagaaccaac agattctata
    18121 tttatagata aaaatagtat atttaatcct ctaaatggaa ctgttataaa atacctagga
    18181 aatctgacca atgcgaaagt tgtaatactt gaaccaaaca aagtactaga tactatgaaa
    18241 attagattga ggcaagatta taataaatta aaagtagaga ataaagaagt aataaaaaaa
    18301 tcatttgctc ttttagaaga aggattgaaa aaaaatcatt tatctctagt tggagaggct
    18361 tgtactttga gtagcttagc aaatgagaat atagaaaaaa aagagtattt aaatgaaata
    18421 ataaaaatat caaaaaaata tggagcatat ggagtaaata ttgctcacag tggaactgta
    18481 gtaggtattt taattgataa gtttatgaat gataaaaaga tgatagatgc cttatgtgaa
    18541 tctaacataa attctgtcta taataaaatt tatacacaaa acataattaa tggaggaatt
    18601 aagggggaaa tagaatggaa tacataaaga atcctatgaa aatagaagaa aaaagttttg
    18661 aattgataca agaaataata gacgaaatta gaccagatta taaatttaag aataatatcg
    18721 aagaaaaaat aataaaaaga gctatacata ctactgctga ttttgattac ttagatatat
    18781 taaaaatatc agaagaagca gttgatagta taatagatgc attaaaaaat aatgcaagta
    18841 tttatactga tacaaatatg gctcttagtg gtataaataa aagaaagtta gaatcattag
    18901 gatgtaagta taaatgttta gtggcagatg atgaaactgt aaaattagct aaagaaaaag
    18961 gaattactcg ttctatggca gcagtagaaa ttgcagctaa agaagaagga agaaaaatat
    19021 tcgttttagg taatgctcca acagctttat ataaagttat ggagatgaaa tcagaaggta
    19081 gattagattt agatgcagtt ataggagttc ctgtaggttt tgtaggagca caagaatcaa
    19141 aagatgaagt tgaaaaaaca gatataccat atataatttc taaaggtaga aaaggtggca
    19201 gtaatttagc agcagcaata gtaaatgcta tattatatac tatgtaggta cctatatgga
    19261 agaatatgta tatatagatg gaaaaaaata tagaagagga tatactacag gttcatgtgc
    19321 aacaggtgca tctaaggctg ctgtatatat gttgattaca aaaaatagaa tcaatactat
    19381 aaatatagat actcctaagg ggataccatt actactaaaa gttgataata taaatatttc
    19441 tgatacattt gtggaatgtt ccataaaaaa agatggtgga gatgatatag atgcaactca
    19501 taccatggat atttatgcaa gagctgaaat tgtagctaaa aatgataaga acaaagggta
    19561 tcttactcta aaggatatag atagtttaag caccaatagt gagtgtaaaa gtgagctcta
    19621 taagtttatt agggtttatg gcggaactgg tattggtgtg gttacgaaaa aaggtcttag
    19681 cgtggatgtt ggtaaacctg ctataaatcc gactccactt aaaatgataa atcatgaaat
    19741 aagaaagctt ataggagata attttgaatc tatacttgga aatgataaag ttttaaaaat
    19801 aacaatcttt gctccacaag gagaaactgt agcaaaaaaa acatttaatc caagattagg
    19861 tatagttgga ggaatatcta taataggtac aactgggatt gttgaaccta tgagtgatga
    19921 tggttggaag aagtctctat ctatagaact tcagatgaaa aaagaacaag gcttagataa
    19981 aataatattg gttccaggaa atcatggaga acagtttata agagaaaaat taaacttaga
    20041 tattaagtat gtagttaggg taagtaattt tataggctat atgattaaag aagctcaaag
    20101 aataggatat aagaaaatct taatggcagg tcatataggt aaatttataa aagtatctgc
    20161 tggtattttt aatactcata gtaaggtagc agatgctaga agtgagatat tagtggcaaa
    20221 cttagcttta atgggagcta gatacgaatt tttaaataaa ataaatcaat gcgtaactac
    20281 tgaagaagct gtagagctaa taaataatag tgaatataga gaagtatata atatattaag
    20341 taataaatgt agggagagag taaagcaata cttgaatgaa gattcagatg atattgatgt
    20401 ggaagtaatc atattttcaa tggataagtc tttattaggt aaatcggata atacagatga
    20461 tttagtggag gtctttatat gataaacata ataggtctag gacctggtaa cttagactat
    20521 ataacaaaaa aaggtgaaaa tttaatttcc acttctgatg tattaattgg tggtaaaaga
    20581 aatttagagt ctataaaaaa ttttgaagga gaaaaaatag ttctggattc taatttaaga
    20641 gaaattatag agtacataaa taacaataaa gaaaagcaaa tatcaataat agcatctggg
    20701 gacccattaa tatatgggat aggtagatat ctatctaaaa atattgataa taagatgtta
    20761 aacatggttt ctgggattag ttcattacag tacatatttt caaaaatata tgtagatatg
    20821 aatgatgtat atattacaag tagtcatggc aaagtaccag attttgatta catattatca
    20881 cataaaaaag tatgtatggt tacagattca aagataggac caaaacaaat aagtagagaa
    20941 ataatagata gaaatctaaa taaaattatt gttgtaggag aaaatctgtc atatgacaat
    21001 gaaaaaataa caatagcaaa accagaagag ataataagaa tggacaattt tgatatgaat
    21061 gttgtggtga ttttagatga ggaatagtga atttataaca ggtaaagttc caattactaa
    21121 ggaagaggtt agagcaatat caataagtaa gttaaattta gtaaatgcta aaaacctcat
    21181 agacataggg gctggtacag gaagtgtaag tgttgaagct gcatataatt atcctaattt
    21241 aaaggttata tctatagaga aaaatgatga tgctatagag cttatagaga aaaataaaaa
    21301 aaaattcaac ttaaaaaata tagaagtgat aaaaggatat gctccaatta atctagggtt
    21361 aaatgttaaa gttgagtcta tttttcttgg gggaacagga aataatctgg aagaaataat
    21421 agaatggtct aaaaaaaatc tcgttacagg aggaagactt gtagctaatt ttataataat
    21481 agatacattt aatcaaactt taaaactgct taaaaagcat ggatttaaag aaatagatgt
    21541 atgtgttttg aatgtttcta aactagagaa actaggaaaa ggtgaatatt ttaaaccact
    21601 aaatcctata tatataatat cttgtgaaaa aggggaaaat gatgatgaat aaagtacatt
    21661 ttgtaggagc aggacctgga gataaagagc tgataacatt aaaaggatac aaattattaa
    21721 gtaatgcaga tgtagtgata tatgcaggtt cacttgttaa tcctgaactt ttggaatact
    21781 gtaaagaaga ttgccaaata cataatagtg cacatatgga tttacaagag ataatagatg
    21841 ttatgagaga aggtatagaa aataataaat ctgttgtaag actgcaaaca ggtgattttt
    21901 ctatatatgg ttcaattaga gaacaagtgg aagatttaaa taaattgaat atagattatg
    21961 actgtactcc aggagtaagt tcttttttag gggcagcatc atctttaggg gttgaatata
    22021 cagttccaga aatttctcaa agtgtaatta taacaagaat ggaaggcaga actccggttc
    22081 cagagaaaga atcaatacaa tcatatgcta agcatcagac atctatggta atatttttat
    22141 ctgttcaaga aattgagaaa gttgtgtcta aattactgga aggtggatat ccaaaagata
    22201 ctcctatagc agttatatac aaagcaactt gggcagatga aaaaatagtt aaaggaactt
    22261 taagtgatat agctgtaaaa gttaaagaaa acaacataaa taagactgct ttaataatgg
    22321 ttgggagatt tttaggagaa gagtataata attcaaagtt atatgataag gatttcaaac
    22381 atgaatatag agggtaatat tgctataatc tgtattacag aaaatggaaa aaaattagct
    22441 ctaaagataa attctctagt aaataactgt gttatatatc aagtaaaaaa taaaaaaagt
    22501 aatttagttg ttgaagaaac atgtgtgcat acagttcaaa agaagttaag tgattttgta
    22561 ggagaaatat ttagcaaatt tgattacata gtatttataa tggctacagg tatagttgta
    22621 cgaagtatag caccatacat aataagtaag tttagtgacc ctgcaatttt ggtaatggat
    22681 gaaaaaggca aaaacgtaat aagtttattg agtggtcata ttggaggggc aaacagcttg
    22741 acacagtaca taagtagtct tattgatgct aatccagtaa taacaacagc tactgatata
    22801 aatcaaaaag cttccttaga tatgatagca aaaaaactaa atgcacatat tgataatttt
    22861 agagaaagtg taaaagaagt aaattctatg ctagtgaata ataaatgtgt tggattatat
    22921 gttgatgaag gatatcaagt tgatactagg ggatttaaac ttatagaaag taaggatgag
    22981 ttaagagata gtttttcaaa cacatatata gaaaaattag ttgtagtaag caataaaata
    23041 aattgtattg aagatattga gaatttaaac ctagataata aatttgaaca ttgctatagt
    23101 acccaaaaaa taataaaggt aattccaaga gatatagtga taggaattgg ctgtagaaga
    23161 aacacagata gtgagctgat gtataattca cttgaagagt ttcttaataa acaaaatata
    23221 gatattagag caataaacac agttggaagt atagaactga aaaaagacga aaaagcaata
    23281 attgaattat caaaaatatt gaaggtgcca tttaaaacat ttttaataga tgaaatatct
    23341 aaggttgata atctttttga taaatctgat tttgttaaaa aaaatgtggg cgtgtattca
    23401 gtagcagaac ctgtagcata tcttttgagc aaaggtaatt tgttagttga aaaacataaa
    23461 tataaaggaa taactttttc agtggggaga ataaaaatat gatatacgtt gtaggaattg
    23521 gaccaggaag taaagataca atgactttag aagcaataaa agtaatagaa gattctgagg
    23581 ctattgttgg atataagact tatataaaac taatagaaga atttatacaa ggtaaagaaa
    23641 taatccaaaa tggtatgagg caagaagttg atagatgtaa acaagcagta gaggaagcaa
    23701 aaaaaggaaa aaaagtagca gttgtaagca gtggtgatgc tggaatatat gggatggcag
    23761 gtcttatttt agaattaata tctaaagaag atgaagatat aaaagtaaag gtaattccag
    23821 gtgttactgc aagtattggt gcagcagcta tacttggagc tcctattatg catgatttct
    23881 gtcatataag tttgagtgat ttaatgactc cgtgggaagt aattgaaaaa agattgagat
    23941 tggctgctga agccgatttt gtaatttgtt tgtataatcc aagaagtaag ggaagaagtg
    24001 aacatctaag caaagcattt aaaataatgg gtgagtttaa aggtggagat acacctgttg
    24061 gagtggtaaa agatgtaggt agagaaaaag aagaaaagtt tgtatgtact tttgaaaata
    24121 tggactttga aaaagtcgat atgactacaa tggttataat aggaaataag tctacctata
    24181 ttaatggtga attgatgata actccaagag gttatacagt atgattttaa ttttaggagg
    24241 aacttcagat agtttatcta tatgtgataa gataaatgaa ctgaaaaatc aaccatatat
    24301 cctttcagtt actacagatt atggaaggga acttgcacaa caacatgcag agaatgtaat
    24361 tttaggtaag cttggaaaag aagatatgct aaaatttata aaagataata atatagtaaa
    24421 aataatagat gccactcatc cttatgcagt tgaagtatcc aaaactgcta ttacttgtgc
    24481 aaaattattg aatatagatt atataaggtt tgagagaaaa tctttaattg aagaaataga
    24541 ttatgataat aaatttatag tagatacaat tgaagaagcc tgtgaaatag caaataaaat
    24601 agggacaaat atattcatag gaacaggaag taaaaatttg aacagattta tagaggaaat
    24661 aagtgataaa aatttagttg ttagagttct tccaacgagt gaagtcatat taagttgtga
    24721 aaaattaggt ttaagtgcag ataatatagt agctatgaaa ggtccattta gccagtatat
    24781 aaatgaagaa acatataaac attacaatat agatttagtc ataacaaaag aaagtggaat
    24841 agctggtggg ttcttagaga aggtaaatgc atgtaaaact ttcaatatac cagtaataat
    24901 tataaaaaga gaatcgatta actacgaaaa tgtatttaat gacataaata aaattaaaga
    24961 catacttgtg taatttttag gaggagaata tatgaagaaa gccttattag tagtgagttt
    25021 tggtacaagc tatcatgaaa cgagagaaaa gacaatagat atatgtgaag ataaaataaa
    25081 aaattcgtta aaaagccatg atttttttag agcttataca tcaaatatga taataaataa
    25141 aataaaaaaa agggatggaa tagaaataga taatccaata caggctttag ataaaatata
    25201 tgaacaagga tatgatgaag taataatcca aacacttcat attatatgtg gcgaagaatt
    25261 taacaaatta aaagaacaag tagatggata ttcttctaaa ttcaaaaaga ttgtattagg
    25321 aagacctttg cttacacata ttgaagatta tcaggaagct gtagaggcaa ttaagcatca
    25381 aattcctcat atggaaagta atgaagcagt agtatttatg ggacatggaa cacttcatga
    25441 atcacattca gcgtatccag cattggaata catgcttagg gataatggaa taaatgctta
    25501 tgttggtaca gttgaagggt atccagaaat agaacatgta ataagaagat taaaagaagg
    25561 aaatataaaa actgtaaatt tgatgccatt tatgttagtt gcaggagacc atgctacaaa
    25621 tgatatggca ggagatgaag aagattcttg gaaaacaata ttagaagaaa atggatttaa
    25681 cgtaagagtt catttaaaag gactaggaga aaattcttat atacaagata aatttgttcg
    25741 tcatgctgta aaatgtgcag agaatgcgaa gttctatggt ataggagcag ggccaggaga
    25801 tggttcttta cttacgataa aagcggttaa tactttaaag aaactggata ttctatacac
    25861 tccagaatca aaaaagggtg gagatagtct agctttatca atagttagtg aatatttacc
    25921 tgaatcagtg gaaataaagt ctaggcattt cccaatgagt tttgatggga atgaaaaaag
    25981 tttagcttgg gacaatgtgg cacaagaaat tatatcagat gttaatgatg gtaaaaatgt
    26041 aggatttgtt acattaggag accctatgat ttatagtaca tatgtatata taatgaaaag
    26101 aatattagga gaggtagaag tcgaaactat accaggaatt tcatcatttt ccaatatagc
    26161 atctaatcag aattttccat tagttatgga taaagaagct ttagtggtaa ttccttgtac
    26221 tatggaagat gataagatag agtatgcttt gcaaaattat aattcaatag tattgatgaa
    26281 agtatataaa aattttaaag aaataataaa aaaattagaa aagtatgatt taatagaaca
    26341 tgctatttta gtaagtaatt cttcccaaga aagcgaaaat gtgtttacag atttaaaaga
    26401 agcgcacctt gaagataaga tatcttattt ttctactata ctagtaaata aagataataa
    26461 aataaaatag gtgaaagcaa tatttaaagg agacttaata tgaatatagt ggttggaact
    26521 aggggtagta atctagcgct tatacaaaca gaatgggtaa taaatgaatt aaaaaagaag
    26581 tatccagaaa ttagttttga gattaaaatc ataaagacaa aaggagattt aattcaaaat
    26641 gtttcactgg ataaaatagg tgataaagga ttgtttgtca aagaaataga gcaacaatta
    26701 ttagatggaa aaatagacat agcagtacat agtatgaaag atatgccttc ttatttagca
    26761 gatggactaa aatttgctca tacgcctaag agagaagacc caagagatgt tctaatactt
    26821 agagaaggat ataaaaattt agatgatttg cctcatggag cagtaatagg aacaggaagt
    26881 aaaagaagga agtttcaatt attgaaacaa agacctgatt taaatatagt acaagttaga
    26941 ggtaatgtgg aaactaggat acgaaaaata aaagatgaaa acatgcatgg tattgtgttg
    27001 gcagcatcag gaataattag agctaattta caagataaaa taagttctta tttacctgtt
    27061 gatgttgtta ttccagcacc tgcccaagga gctttagcta tagaaattag aagcaatgat
    27121 agtacgatag agggaatagt aaattcactg aaggatgaga atacagaaat ccaaatattg
    27181 gctgaaagag gttttttaga tggtgtaaat ggaagttgtc atattccaat ggcagcttat
    27241 tgtgaaataa tccaagataa aatacatctt actggcttat atggagattc agaaggaaaa
    27301 aaagtagtaa tcaagtctat agatggagat atttcttctc ctagagagtt aggtttaaag
    27361 ttagctaaac ttgtattgaa ggagtatgag aattatgaag ggtaaggttt acttagtagg
    27421 ggcaggacca ggtgattata aactcatgac tttaaaaggt ttagagtgta taagaaaatc
    27481 ggatgtaata gtatatgata gattagcaaa tagtagttat ttaagagaag ctaagcctga
    27541 ttgtgaactt atatatgtag gcaaggcatc aagtaatcat attttgccac aagaagatat
    27601 aaatagaatt atagcagaaa aagctaaaga aggaaaaatt gtaactcgtc ttaaaggagg
    27661 agacccttat gtatttggta gagggggaga agaagcagag actttagtag atgaaggtat
    27721 agaatttgaa gtagttccag gtataacttc tgctatagga ggtttatgtt atgcgggtat
    27781 tcctataaca catagagacc atgcttcgtc atttcatgta ataacaggtc atttgaaggg
    27841 tgatgatagt ggagaattaa actggaatgc tcttgcaaat aacaaaggta ctcttgtgtt
    27901 tttgatggga atttcaaacc tagagaaaat aagtgaaaac ttaatgaaag aaggaaaaga
    27961 taaggataca cctgttgctc ttataagttg ggcaactaga tataatcaaa gagttgttac
    28021 atctacactt gaaaatgttt atgaaactgc tattaaagaa gaagtaaaac cgccaactct
    28081 tatagtagtt gggagtgttg taggacttag agaaaagttg aacttctttg aaagtaagcc
    28141 tttatttgga aaaagtattg ctgtaactag gtctagaaat caaaatagtg tattggtaga
    28201 aaaaataatg gatttaggtg gaaatcctat agaaatacct actattaagg tagaaaaaat
    28261 acaaaataat attaacttag aaaatgaaat aaaaaatata aataagtata attatctaat
    28321 actaactagt aaaaatgccg tagaaatctt ctttgaaaag atatatgaaa tgaattttga
    28381 tttaaggata ttatctaatt taaaggtatg tgctataggt tctgctacat caaatgagtt
    28441 gaaaaaaaga ggaattatag cggatatagt accaaaaaaa tttgtagcag agtcactgta
    28501 tgaagagtta gcgcctattt taaatgatga cgataatata cttataccta gagcagaaaa
    28561 tgctagagat tatctggttg acaaacttaa ggctatttgt gaagtaacag aagtacatat
    28621 atatagaaca gtaatagatg aggagaaaaa aggtgagata ttagatattt taaattctaa
    28681 tgacatagat tatataacat ttactagttc ctcaacagta aataatcttg ttgaaattat
    28741 aggaaaagaa aatattaaaa tattagaaaa aataaaaaca ctatctatag gtcctataac
    28801 tacagagaca ataaatagtt tgggaataaa attgtacaaa gaatctgcta catctactat
    28861 agattcgtta gtagatttaa ttataaaaaa ttaattttgg aggcttgaat atgttaagaa
    28921 gaccaagaag actaagagca agtaaagcaa tcagaaattt ggtaagagaa actaaggtga
    28981 atgtagaaga cttaatatat ccactgtttg tagtagaggg ggaaaatata aaagaagaaa
    29041 ttaaatcttt accagatgtc tatcatttat caatagacat gctagaagac gaaataaaag
    29101 aaataagtga gttgggaatt gaacatataa tgttatttgg tgttccagat gaaaaagatg
    29161 agtgtgcttc acaagctttt aatgataatg gaatagttca aaaggcaatt agaaaggtaa
    29221 aagaaataaa ttcagatata aatgtaataa cagatgtctg tatgtgccaa tatacaagtc
    29281 atggacattg tgggatttta actgatactg gttatgttga taatgataaa tcattggagt
    29341 atttggctaa aatatctgta agtcatgcta aagcaggagc tgatatggtt gctccatctg
    29401 atatgatgga tggaagaata caagcaataa gagaagcttt agatgaaaat ggattagaaa
    29461 atgttggaat catggcttat agtgttaagt atgcttcaaa ttattatgga ccttttagag
    29521 aggcagctca ttcagcacca tcttttggag atagaaaaac atatcaaatg gacccagcaa
    29581 atagcaatga agctataata gagactgaac ttgatatatt ggaaggtgca gatatactta
    29641 tggttaaacc tgcattatca tatttagata ttataagaag agttaaagat aattttgact
    29701 tacctctagc agcttataat gtaagtggag aatactctat gcttaagttt gctgtgaagg
    29761 aaggtttatt aaatgaagat gtaattcttg aatcaattac atctataaaa agagcaggag
    29821 cagatataat aataacttat tttgctaaag acattgctaa actgttgaaa aaataaattc
    29881 tagtagtata aagaatatca aaaagtatag gaattgtaat taactattat acagtagttt
    29941 tgtttagaca tttattgaag aataatagaa actaatttgc aatttctata ctttatttat
    30001 gtaactaaat aaaatttgga ggtagaagat gctatatcct attaatttaa aattagatga
    30061 gttagatgta gttataattg gtggtggaga agttgcatat aggaaatgta aaaatttttt
    30121 agagtttaac aaaaatgtta ctatagtttc taaacaaatt ttaaataaat tttatgattt
    30181 aaaaggaaat ataaaaataa taaaagatga ttataaagaa atatatataa aagatgcttc
    30241 tgtaataatt gctgccacaa ataatagaga gttaaatatg gaaataggat tatattgtag
    30301 agcaaaaaat aagctagtga atgtagttga taatatagaa atatcgaact ttactgttcc
    30361 atcatatata aaaagaggag atttacttat aagtgtgtct actgggggca agagtccttc
    30421 attatcatca aagataaaaa aagaaattga agagagatat actgaagatt atgaggagta
    30481 tttaaatgta ttaggagata taagaaaaaa ggttataaaa aaatacgaag ataaaagtaa
    30541 aagaaaagat gtgcttaata tgttggtaac acttgaccta gaagaactta agaagtttga
    30601 tatataaaaa ataaaataaa aaatgttaaa aaggtgttga cggattattt ttaagatgat
    30661 atagtattac ttgtccttaa gaaaagggca aacataa
//
LOCUS       DBGJFF010000037        29791 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.037, whole genome shotgun sequence.
ACCESSION   DBGJFF010000037 DBGJFF010000000
VERSION     DBGJFF010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29791
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.037"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(186..2852)
                     /locus_tag="ACOIAF_001704"
     CDS             complement(186..2852)
                     /locus_tag="ACOIAF_001704"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HGZ2668718.1"
                     /translation="MENTNLSKTYNPKDFEARLYKKWMDEGYFKSKPNPDKKPFTIMM
                     PPPNITGQLHMGHALDHTLQDILIRWKRMDGYEAFWLPGTDHASIATEVKVVERIKKQ
                     EGKTKYEIGREEFLKRAWEWKDEFGGKISNQLKQLGDSCDWDKERFTMDEGCNEAVIE
                     FFVSLYEKGHIYRGNRIINWCPDCKTTLSDAEVEHEEHDGNFYHIKYPLKDSEDFLEI
                     ATTRPETMIGDTGIAVNPEDDRYKHLIGKTAILPLVGRELPIVADSYVDLEFGTGAVK
                     MTPAHDPNDFEVGLRHNLEQLNTMNEDGTMNEVCGKYEGMDRFECRKAIVADLKEQGY
                     LIKIKEHNHNVGTCYRCHTVVEPRLSEQWFVKMEELAKPAIDILKKGELEFVPDKFDK
                     TYLQWLENIRDWCISRQLWWGHQIPAYYCQECGEIVVARKMPDKCPKCGSTHFKQDED
                     ALDTWFSSALWPFSTLGWPNKTEALDYYYPTSVLVTGYDIIFFWVVRMAFAGMFCMNE
                     KPFDHVLVHGLVRDSQGRKMSKSLGNGIDPLEIIEQYGADALRFTLTTGNSPGNDMRF
                     YMERVEFARNFANKLWNASRFVFMNIDEDIIKNMTRESVKEDLTLADKWIISRANNIV
                     KEATNNMDKFDLGIALQKIYDFTWSEYCDWYIEMVKPRLYGEDANAKSAALYTLTYVL
                     EKILKLLHPYMPFITEEIYTHLPTVEGCIIVSEWPKYNEEDNMAEEEDMMNLLMEGIR
                     SIRNVRAEMNVPPSKKAKLIIIPSEEKIEAIELGKDYFITLASASNVEIAKDKSNVPE
                     DAVGVVIDGVEIFIPLNELVDFEKEIERLSKEKKKLEGEIKRVNGKLANQGFLAKAPE
                     SLIEEEKAKKEKFEEMIKSVEERLTNLESKIK"
     misc_binding    complement(2928..3190)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(3486..4313)
                     /locus_tag="ACOIAF_001705"
     CDS             complement(3486..4313)
                     /locus_tag="ACOIAF_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HGZ2668719.1"
                     /translation="MKIKDNKAVFHVFIRIFIIAVLILTGTLLEEKINNVPVENISSK
                     GENTLELLSQSNTLGEIIYKASQEETKGPGATSEKVAYITIDDGPSKYTNALLDILKE
                     NDVKATFFMLNQNMKNHKEEVRRVLEEENSIGFHSVSHDIHKLYKSPEVTVGEFNTCN
                     NTLQEITGQSSKLIRLPYGSKPYMPEGSYNKLMANDYLIWDWNLDTQDWKSSTSQIVS
                     NILYYGRKRDELVVLIHEKEQSLNALNNIIRILKERGYTILPITENITPKNFWQENL"
     gene            complement(4361..5797)
                     /locus_tag="ACOIAF_001706"
     CDS             complement(4361..5797)
                     /locus_tag="ACOIAF_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HGZ2668720.1"
                     /translation="MNTRNDNSKILEIYKMKLFGELVKAVWNDDIEDIENVPNKILNE
                     DLKDNLNNYFSGKFDKDTMVNLIRVTMGLDVTEDTDKNLKESLHEAVNLDEVKRPIIS
                     VISDACKYCDENNKEDESCKIRSKHINCNEEDVCSACGDCVSRCKLGAISDKIEFIPM
                     IKLLKDIKSPVYAIVAPAFVGQFGKDVSPGKLRTALKKIGFEDMIEVALAADMLTAKE
                     AYDYYEHMKKDEEGCFITSCCCPIWVSLIQNSFPQILENVSPSVSPMIACGRAVKILN
                     PDAKVVFIGPCMAKKKEAMLEDIKDAVDFVLTFKELQEIFDALELNPAIMDESNRVEA
                     SLSGRVYARTGGVSKAVKLSVRRIDKNIKFEEKAFEGTKECMDGLKKVLNKEIEATFI
                     EGMGCVGGCVGGPKRILSVEEGTKNVDEYSESTNMETPFDNLNVLQFLTSLGIKRVES
                     LGEKEEEKVKKIFSRDIKSNKKENKYKK"
     gene            complement(5884..6753)
                     /locus_tag="ACOIAF_001707"
     CDS             complement(5884..6753)
                     /locus_tag="ACOIAF_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HGZ2668721.1"
                     /translation="MKLQKMGMRTIKTGISVTLCMILGDYLVENMFYSLVACIISVQD
                     TVKGSLKTGINRVEGTILGGIIGFIFAIIKAGDPFLCGLGVMCSIYLCNLFKIKSVVV
                     ACVTFLAIHLGVGNSDPVYYSLHRVLDTSVGVLFGVLVNYYVARPNYLNSVIDEFKKI
                     EELSMLLIEDKILRKENLNVEKFEEEVEKLEEIYSKLIDELDYSLSETNIEKIENALR
                     ICREIHFHMQSIELLENKLYLKEHTYKYLKKTYNVDKLDWNIDEEKSPVFNYHLMKIM
                     IEIELLRKLNKSY"
     gene            complement(6911..7564)
                     /gene="phoU"
                     /locus_tag="ACOIAF_001708"
     CDS             complement(6911..7564)
                     /gene="phoU"
                     /locus_tag="ACOIAF_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HGZ2668722.1"
                     /translation="MVNNSLDISIDTLKEQTVLMMEKCEEAIEKSVTCMIEKDLEGAR
                     KVVEDDEEINKLREEIRDKSIELIVLKQPMARDLRFIYSLGNISIELERIGDYAANIA
                     REMLKIGGEAYIKELIDIPKMAKECKSMMSQTREALILFDASIAYDVGTKDDIVDGLY
                     EQVQIDCLKIMHENSNTIDQGVKLLLIGRYLERIGDHITNVCEKIIFAIEGKMVEIG"
     gene            complement(7592..8356)
                     /gene="pstB"
                     /locus_tag="ACOIAF_001709"
     CDS             complement(7592..8356)
                     /gene="pstB"
                     /locus_tag="ACOIAF_001709"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HGZ2668723.1"
                     /translation="MELIDKIKMSVKDLDLFYGDKQALKKINMDIKENKVTALIGPSG
                     CGKSTFIRTLNRMNDLIEDVTIKGNISVDGEDIYTSDDVINLRTKVGMVFQKPNPFPM
                     SIYDNVAYGPRTHGLRDKKQLDKIVEESLKGAAIWDEVKDRLKSSALGLSGGQQQRIC
                     IARAIAMRPEVILMDEPTSALDPISTLKVEELIEDLKKDYTIVIVTHNMQQAARISDE
                     TAFFLNGEVIEFSDTKTMFTTPVDKRTEDYITGRFG"
     gene            complement(8375..9199)
                     /gene="pstA"
                     /locus_tag="ACOIAF_001710"
     CDS             complement(8375..9199)
                     /gene="pstA"
                     /locus_tag="ACOIAF_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HGZ2668724.1"
                     /translation="MRKFKENLLKSLVYLSALFTIVSLVVIVGFIFIKGIGNMNFNFL
                     FSNYSASGDGGILPMIITTLYTVVVSIVVATPIGILAAIYLQEYAKKGKAVTAIRFAT
                     ESLSGIPSIVYGLFGGIFFVVTLKMGYSIVAGSLTVAIIILPVIIRTTEEALKTVPQA
                     YRESSLALGATKFQTLYKVVLPSAIPGILSGVILSVGRIIGESAAILLTAGTVAKMPG
                     GIFDSARTLTVHSYLLTKESGDIATAASIGIVLIVIVLALNMLARFVAKKLNKANY"
     gene            complement(9201..10118)
                     /gene="pstC"
                     /locus_tag="ACOIAF_001711"
     CDS             complement(9201..10118)
                     /gene="pstC"
                     /locus_tag="ACOIAF_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HGZ2668725.1"
                     /translation="MIVKSRLVGLEKNDNNGNKTKYVIEKVAKNIFLLSALVAVASLL
                     LIIGFVFYKGLRPFIFEGYSFIDFLTGADWVPSANKFGISSMIVASIFATLGALVIGV
                     PVGILTSVFIAEVAPKRLAKIMSPAVELLAGIPSVLYGVFGLAVIVPTIQSIFNLPKG
                     QSLLAIVIVLAVMMLPTIISVSETAIRAVPKAYKEGSLALGASKIETIFKVVLPAAKS
                     GVLAAVVLGVGRALGETMAVILVAGNSPVMPTAITDSVRPLTTNIALEMGYAFGTHQE
                     MLFATGVVLFTFILILNLVLNKLSNKAVN"
     gene            complement(10499..10945)
                     /locus_tag="ACOIAF_001712"
     CDS             complement(10499..10945)
                     /locus_tag="ACOIAF_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668726.1"
                     /translation="MAISDLAFGTECLKTSFQMLFSVISLVNINLLQAQILIAIGGLI
                     SILAMVSLTLFLSAKCKDSLTSMLIAFSICLIPMGIGVSWIGYLLPPGGIGFKISLLY
                     QMTSFNFLHLGGMSFWTPHIILFFSVIEIPIFIFLAIRTYCKHQVS"
     gene            complement(11463..12143)
                     /locus_tag="ACOIAF_001713"
     CDS             complement(11463..12143)
                     /locus_tag="ACOIAF_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGZ2668727.1"
                     /translation="MQTKILIIDGDKDNCKKLKGFLEEKGISIDLAYNCEEAIGKIFS
                     NKYDLIFLEIILSDGDGWTLCKKIRNVTTCPIVYMTYINEDQSILNALNSGGDDYLIK
                     PLNLEILYAKVKAILRRMNSYVNNNENNTKLEEYNRTSGVIKLEDKIIKLTPTENKLL
                     NFFIENPEKTLTTKEIYEHVWMNEYLEDNYSVVVAVNGLRKKIEKDYKNPQKIITIRG
                     AGYYFNKE"
     gene            complement(12193..14208)
                     /locus_tag="ACOIAF_001714"
     CDS             complement(12193..14208)
                     /locus_tag="ACOIAF_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGZ2668728.1"
                     /translation="MKKYNILKGTLIVLIVSLLAITGLNLLYKYDNKYTYKSIRASNG
                     ILTIDNDTFTKGKLVFLIDDWEFYNHKIFKPNDFTEQDIKPEYVFIGQYPDFSMRKGN
                     QSPYGNATYRMRIKNEGKDKLLSLELPEIFSASEVWINGEMVSKLGDVGTEKYRPKIK
                     NSVVSFIAKKNTEIIINVSNFSHYYSGLYYPPALGETKDISNMMFYRLLFYSIICFTT
                     FAIAIFSLSVWFLSNKSKIYLYFGCMCVFFAIHVSYPFVHLLGLPLVSFTYAVEDLSY
                     FMVVLCIICINGSISKIEENKVYRFLILPLGITMSIISIVIPIILLPYDMYFVNIYGK
                     LIDIYKYIVFIYILMSSLVSIYKKTCSEHISSYILVSVNTVFGISILFDAVTSNRFEP
                     IFTGWQTEYCGFVIVILFSVMMINRNRIIIKENEKLTRNLEHEVEVRTNELTTLLNER
                     KRFLADVAHDLKAPVSAIQAFIDLIKVGNIHVDEETRTYLYAINQKSNEVQNRVRSLQ
                     EFTSQDKSIGISEKICLNNFIKEIYENNLPDTQACGVNFETYVLEKKIYAKINKEMLT
                     RAFENLIYNALTFTPFDGKIILSMDKEDGFAIIKISDNGKGIPPENIPKVFDRFFTER
                     DNNEPKGQGLGLYIVKSIIREHGGEIFVDSELGKGTVFTIKLSLCFL"
     gene            complement(14280..14957)
                     /locus_tag="ACOIAF_001715"
     CDS             complement(14280..14957)
                     /locus_tag="ACOIAF_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGZ2668729.1"
                     /translation="MYNLLVVDDDVDILNINQIYFSNIGFNVYTSETAKDAMDIVESI
                     VLDCIILDISLPDIDGYKMCNDIKEKVNIPIIFLSNYEHEEEKVRGFLAGGDDYITKP
                     YSLKELELRIYARMRQYKNITSVPNVLKFSALVINVNSRKVTFDGESIDLTTMEFEIL
                     LFLAENKEQVFSKIEIYNQVWKMPDIGDQHTVQVHIAQMRKKINSLSREHQYIQTVWG
                     KGYKFVP"
     regulatory      complement(15537..15620)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            complement(15853..16695)
                     /locus_tag="ACOIAF_001716"
     CDS             complement(15853..16695)
                     /locus_tag="ACOIAF_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HGZ2668730.1"
                     /translation="MFKKKIGVLLASTMLVGALAVGCSSNSGGSGDSGDSSKVSVSGS
                     TSIGPLMETIGEKFQEKNQGVSVEVQQIGSSGGIKNAIEGTSEIGMSSRDLKDEEKSA
                     GLKETKIAIDGIALITNTANSVKNLTMDQVKGIYTGKITNWKEVGGNDAPIVIVSREE
                     GSGTRDGFQEIVGFDSEELTKDAQISDGSGNIKTTVEGNENAIGYISFGYVDEKVNSL
                     KINDVEATDANVIANKYPISRPFILVNKEDGLSEKGKALIDYILSDEGQALVSEKGFI
                     KVNQ"
     gene            complement(16947..18731)
                     /gene="pepF"
                     /locus_tag="ACOIAF_001717"
     CDS             complement(16947..18731)
                     /gene="pepF"
                     /locus_tag="ACOIAF_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HGZ2668731.1"
                     /translation="MGTDRSTIEEKYKWKIDKMYSSKEEIEKDISKVKNLIQEVKEYK
                     GKLSESKENLYKVLNISENASRIVQNLYVYTHMKQHEDTRINDNQGRATKTEMLSTDL
                     GVATSYIVPEILAIEENKLSEYLKDEKLSFYTKYIKDILRDKPHTLSEREEEILAATS
                     ELSTIPENVYDMLAYADMEFPEIEDEEGNKVKLSHSNYSLFIKSKNSRVRKDAFEGEF
                     STYEKYKNTFASTLYGGIKSEIFYAKTRKYNSAIEASLFSDDVSLDVYNNLISAIDKS
                     IPNLNKYVELKKKFLGLNEIHMYDLYVPLTDKFDMDIPYDKAQDIILEALKPLGEEYL
                     KVIKSAFDEGWIDVYDNEGKKGGAYSWGSYDSHPYILMSYKNDLNSLFTLIHELGHSV
                     HSHYSRTSQPFLYSDYKIFVAEVASTLNELLLINYLLENSKSKDETIYLLNYYLEQFR
                     TTVHRQTMFAEFEKIVHQRVESGEPLTADEFTNIYYKLNEKYYGKSAIVDKQIGIEWA
                     RIPHFYSNFYVYKYATGFSAASALSQQILSEGSTAVDRYINFLKSGGSEYPLEQLKSA
                     GVDMTKKESIEEALNVFAELVNKLEKEL"
     gene            complement(18797..19462)
                     /locus_tag="ACOIAF_001718"
     CDS             complement(18797..19462)
                     /locus_tag="ACOIAF_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HGZ2668732.1"
                     /translation="MIDIGSKEIAIRLLLALIIGGIIGLEREKIRQFAGFRTHILIAI
                     GSCIVSITSIQLFFDYNLYTNADPARMPAQVLSGIGFLGAGAILKNKGGVKGLTTAAG
                     MWATACIGIAVGYGYYELAILGGLFVMIALFVLRIINTFLFSQEKNVLILKLSNLDSI
                     PFLYDKFEKNQLIVRNMEIECRNEEYWKVNFFVSYAKRIHLHDVMKEINMIEGVINVD
                     YLD"
     gene            complement(19613..19774)
                     /locus_tag="ACOIAF_001719"
     CDS             complement(19613..19774)
                     /locus_tag="ACOIAF_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl-phosphate phosphatase Spo0E family
                     protein"
                     /protein_id="HGZ2668733.1"
                     /translation="MANNELEALKNEIEAVREEINTYIEYPEIFQEELVESSKKIDIL
                     INKYMYLSK"
     gene            complement(19852..20361)
                     /locus_tag="ACOIAF_001720"
     CDS             complement(19852..20361)
                     /locus_tag="ACOIAF_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HGZ2668734.1"
                     /translation="MKTRKMTFLGLMIGYSLALYVLETYIPNPLIVIFPGAKLGLSNI
                     ITLISLMLLGVKDTVIIVTIRVILSSIFAGPLSYLLFSIGGAYLSLLFMYLASKIKDL
                     SLIGISIIGAIGHNIGQLIVASLIIENILAIGYLPFMLMASLVTGMFVGLVSRYTIPK
                     LKHFYSKFY"
     gene            20567..20785
                     /locus_tag="ACOIAF_001721"
     CDS             20567..20785
                     /locus_tag="ACOIAF_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HGZ2668735.1"
                     /translation="MTVYDLKIGEKGFINSIDGDIKLSKRLLALGCINSTEIEVKKVA
                     PLGDPIIIRFRGFDLAIRKSDAKNISLQ"
     gene            20803..22749
                     /gene="feoB"
                     /locus_tag="ACOIAF_001722"
     CDS             20803..22749
                     /gene="feoB"
                     /locus_tag="ACOIAF_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HGZ2668736.1"
                     /translation="MINVALLGNPNVGKTTVFNLLTGSNQYVGNWPGVTIEKKEGFLG
                     KEIKVVDLPGIYAMDTFSNEEKVSKSYLENEDVDVIVNVVDASNLSRNLYLTTQLMQF
                     NKPIVILLNMLDIAESKGVNIDAKKLSEELGVIVVPIIAKKKNGVDRIEEEIKGATQK
                     AFVYQKNFGSETETYKKLESILSRCTKTTYTQKKSISDKIDNIVLNPILAYPIFIGVL
                     FLLFKFTFDWVGGPLQEGFASLIEAYISAPVSDMLSNSSPWFKSLIVDGIIGGVGGTL
                     PFFPLIFTLFFGISLFEDSGYMSRTAFLMDKVMRKVGLSGKAFIPIVMGMGCSSPAIM
                     ATRTLESEKDRRVTALIAPLMTCGAKLPIYALFVAIFFPHNAALVTTSLYLVGIVMAI
                     VVALFLNKTAFKTEAEPFILELPEYRIPTIDALIKNTWNKSKGFLIRVVTVMFAMSVV
                     IWGLSSFNVFGYTEDINVSFLATLGHIISPIFKPLGFDDWRTSVAILSGLGAKEIVVN
                     SLNILYGNLTVVLPTIFNGVTAYTFLIFTALYTPCIAALATMKKEYGNKMMFTSFAYQ
                     FILAWIMAFIVKSIGGVLFMGNSIMEALIGGIIIVLALFILFNKFKSTKNGNVCSGCS
                     ICSGCPSANSCSSKEDSNKKNNAH"
     gene            complement(22928..23983)
                     /locus_tag="ACOIAF_001723"
     CDS             complement(22928..23983)
                     /locus_tag="ACOIAF_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HGZ2668737.1"
                     /translation="MTIQDYMLETPVRMREIISNADSLFNEVKRTNLKKIIITGSGTS
                     YHSGVQVQPYLQNLLDIDVVKMYPFMITEDTFKFDNENTLVVGVSQGGSSYSTYNAMK
                     LAEDKGCKIASMAGCKNALIDEISDYILTVNCGEEKSGAKTKGYYCTKLNLMLLGLQI
                     AREKGIISSEKYNEEINKILDAINRFEAVYKLSKQWIERNKEKLVNSKEIRIIGHSDI
                     YGDTLEAALKLLETMRIPVTGYEFEEFIHGIYNAINSDSTIFILDTGKEPRVTKMIDV
                     LSGWTENVFAIGRDVTENDKNLKIDITDNPYYQTFNFIVPIQLICGEIPTLRGVDPSV
                     PKDTRFHMKLGSKKLNK"
     gene            complement(24031..24831)
                     /locus_tag="ACOIAF_001724"
     CDS             complement(24031..24831)
                     /locus_tag="ACOIAF_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HGZ2668738.1"
                     /translation="MNKLTKKELRSMFWRSFALQGAFNYERMQNLGYCYSMLPAIKKL
                     YNKKENQAKAIERHLEIFNTTTVVVPAILGITAAMEEENANNPEFDESSISAVKTALM
                     GPLAGIGDSLFWGTFRIIAAGVGVSLAKEGNIFGPLLFLLLYNIPAFALRIFGLKYGY
                     QVGVNSLERIQREGLMEKIMSMATTVGLFVVGGMVATMLSITTPLKFNLNGAEVILQD
                     ILDKIIPNMLPLTFAFVIYYMLKRKVSVTKLTIGTIVTGIALHAIGLL"
     gene            complement(24824..25615)
                     /locus_tag="ACOIAF_001725"
     CDS             complement(24824..25615)
                     /locus_tag="ACOIAF_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HGZ2668739.1"
                     /translation="MLVQATMLAIIAGVGILDGRIFGQSMLDRPIVTGMLVGLVLGDI
                     KSGIMIGAQLELIWMGIAGIGAATPPDVVTGGVLGTAFAILSGNGAEVALAVAVPVAV
                     LAQSLGVLVRIINSYFSQKAVFYAKQADFKKVTIMMWLPVILFFLSTSVPTFLAIMIG
                     ADKVTALINAIPKVILDGLGVAGTLLPAVGFALLMDMLLSKKMSVFFFIGFLLASYGG
                     LDITAIALFGVCVAFVLNFYINNKENNQNPQLAINTLTEGEIDFE"
     gene            complement(25655..26077)
                     /locus_tag="ACOIAF_001726"
     CDS             complement(25655..26077)
                     /locus_tag="ACOIAF_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGZ2668740.1"
                     /translation="MKKFLIATHGELSRELVDTSKLIIGSLTNIEYFCMTTDKSADDA
                     EKEIRSILSNNKDSEEFVVLTDVFGGSVANICTNLLLQGFKFELLTGVNLPMLLSILL
                     LVEEDTKLIVKSGLEEAKNGIVYVNELLENQNKIKNVM"
     gene            complement(26093..26572)
                     /locus_tag="ACOIAF_001727"
     CDS             complement(26093..26572)
                     /locus_tag="ACOIAF_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HGZ2668741.1"
                     /translation="MVSLMRIDDRLIHGQVAYGWATSLGINVILVANDEAKNDQMKAM
                     SLNLAKPSNVTLYIRGVEESGEIVEKFASSKKSNVLVLVKNTKDALVVAKNSGGVLKE
                     INVGGLRYEEGKKKLTDLVAVDDDDINNFKQIGDLGIDIEFRMLPRDKKKQLSDLIK"
     gene            complement(26704..29460)
                     /locus_tag="ACOIAF_001728"
     CDS             complement(26704..29460)
                     /locus_tag="ACOIAF_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HGZ2668742.1"
                     /translation="MRDEILSFVNECVLFNNFDELKKESGIITEDIVKKFNLSRTQSS
                     RLLNDLVKSDNLIKINSRPVIFLPKNKINDVVGPTKKSIYENLDELKNECKLLKQETV
                     FNSIIGYDNSLKEVIEQIKTAVLYPNGGLTIMLTGESGVGKTFLAKIIYKYSVSSNVL
                     EKNSPYNVLNCAQYYNNLELLSSLLFGHVKGAYTGANESRAGLIEQSDGGVLFLDEVH
                     RLNSEGQEKLFTFMDTGTFSRIGENGVIRKARVRLIFATTESLSEFLQTFLRRIPINI
                     YVPNIEERGTIEKRKIVEHFIYEESKVLGLPIEITQITLNAILKIKFKGNIGECKNVI
                     KYACGRAYAKNQRRNGNIFVTLQDLPRNLYIENVDIFQFQSGKSKSIIFSPKQFSIEM
                     SNGRNKRNIIYQSFIKCLDYFDRLEKNEITKEKFSKKSSNLMIRLMDNLVFSNNDENN
                     NVLMELIISIMQDIFRNLEVNNNIKYDGDNVLAFSSYLYLKDESYQLSDEYKALENKL
                     LIYLKKESYKEYSIISKISTLITNRLDMYLNNVDTIIMIIFIKSTIIRMKFNQVNATI
                     IAHGYATASSMANVCNRMLGKNIFTAIDMPIDATIADVSKQMSRYLEGDRIKNGLIVL
                     FDMGSLNLIYEDLKNKINSPMLFIDQISTISALEVGNLLMQGKNIEEIAEIMDEETKP
                     NIQLYYPQKNKEYAIISSCFTGIGTAIKIQHLLSESLIGIVDVKIIPYDYDSLVSMGD
                     KEPVFEMYDVMAIVGTANPNVNGVDFILLEDIISGKGEDDVFRIFSRVVENEKIKNIN
                     DSIVKNFSLIRVIDSLTILDSKKIIERIEEGINAIENIQKKKLSNDKKISLYVHLSCM
                     IERLVRQTEIEEYTDMDLLIKNHHSEVKLIKNAFSVLEKSYSVQIPISEIGYIYNIIY
                     GLPQ"
BASE COUNT         9587 a         5250 c         3240 g        11714 t
ORIGIN      
        1 atcataatat atcaaatttt tattgcttat aaattttaag acataaaatt catatattca
       61 caacataaaa taaaaatata acacatctgt aaagaattat aacatcatat ttgtatatat
      121 ataaagaaaa acatacaaaa agtatagaat atatactttt tgtatgtttt tactaattct
      181 tatatttatt ttattttaga ttctaaattt gttaatcttt cctcaacaga tttaatcatc
      241 tcttcaaatt tttctttttt tgctttttct tcttcaatta aactttcagg agcttttgct
      301 aagaaacctt gatttgcaag ctttccatta actctcttta tttcgccttc taactttttc
      361 ttttctttag ataatctctc aatttctttt tcaaaatcaa ctaattcatt aagaggtata
      421 aatatttcaa caccatcaat aactactcca actgcatctt ctggaacatt agatttatct
      481 tttgctatct ctacatttga agcagatgct agagttataa aatagtcttt accaagctca
      541 atagcttcta ttttttcttc acttgggatt attatcaatt tagctttctt agaaggtggt
      601 acattcattt ctgctctcac atttcttata cttcttatac cttccatcaa taagttcatc
      661 atatcttctt cttcagccat gttatcttct tcattatatt ttggccattc agatactatt
      721 atacatcctt caacagtagg aagatgagta tatatttcct ctgttataaa tggcatgtat
      781 ggatgtaata attttaatat tttttcaaga acataagtta atgtatataa tgctgcagat
      841 ttagcatttg catcttcccc atataatcta ggtttaacca tttcaatata ccagtcacag
      901 tattctgacc aagtaaaatc gtatatcttt tgaagtgcta ttcctaaatc aaacttatcc
      961 atgttattag ttgcttcttt tacaatatta tttgctcttg atattatcca tttatcagct
     1021 agagttaagt cctctttaac actttctcta gtcatgtttt taattatatc ttcatcaata
     1081 ttcataaata caaatcttga tgcattccac aatttatttg caaagtttct agcaaattct
     1141 actctttcca tatagaatct catatcattt cctggggaat ttccagttgt taatgtaaat
     1201 cttagggcat cagctccata ttgctcgatt atctccaatg ggtcgatacc atttcctaaa
     1261 gatttactca tttttctacc ttgagaatct cttactaatc catgaaccaa aacatggtca
     1321 aatggctttt cattcataca gaacatacct gcaaatgcca ttctaactac ccagaagaat
     1381 attatatcgt atcctgtaac aagtacactt gttggataat aataatctaa agcttcagtc
     1441 ttatttggcc atcctagagt agagaaaggc cataatgcag aactgaacca agtatctaag
     1501 gcatcttcat cttgtttaaa atgagtgctt ccacatttag gacacttatc tggcattttt
     1561 ctagcaacta ctatttctcc acactcttga caataataag ctggtatttg atgaccccac
     1621 cataattgtc ttgatataca ccaatctctt atattttcta gccattgtaa ataagtttta
     1681 tcaaatttat ctggaacaaa ttctaattct ccttttttaa gaatatctat agctggtttt
     1741 gcaagttctt ccatcttaac aaaccattgc tctgataatc taggctcaac tactgtatgg
     1801 catctataac aagttcctac attatgatta tgctctttta tcttaattag gtaaccttgt
     1861 tcttttaagt ctgctacgat agcttttcta cactcaaatc tatccatacc ttcatatttt
     1921 ccacaaactt cattcatagt tccatcttca ttcatagtat ttaactgctc aagattatgt
     1981 cttaatccta cttcaaaatc attagggtca tgtgctggag tcatcttaac tgcaccagtt
     2041 ccaaactcta aatcaacata actatctgca actattggaa gttctcttcc tactaatgga
     2101 agtattgctg tttttcctat taaatgttta tatctatcat cttctggatt tactgctata
     2161 ccagtatctc ctatcattgt ctcaggtcta gttgtagcta tttctaaaaa gtcttcacta
     2221 tcttttagtg ggtactttat atgataaaag tttccgtcgt gttcttcatg ttcaacttct
     2281 gcatctgata aagtagtttt gcaatctggg caccagttta ttattctatt tcctctgtat
     2341 atgtgacctt tttcatatag gcttacaaaa aattctataa ctgcttcatt acacccttca
     2401 tccatagtaa atctttcttt atcccaatca caagaatcac ctagttgttt aagctgatta
     2461 gatatttttc ctccaaattc atctttccat tcccaggctc tctttaaaaa ttcttctcta
     2521 cctatttcat actttgtctt gccttcttgt ttttttattc tttcaacaac tttaacttct
     2581 gttgctatag aagcatggtc agttcctgga agccaaaaag cttcatatcc atccatacgt
     2641 ttccatctga ttaatatatc ttgaagtgta tggtcaagag cgtgtcccat atgcaattgc
     2701 cctgttatat ttggtggtgg catcattatt gtaaatggtt ttttatctgg atttggttta
     2761 gatttaaaat acccttcatc catccatttt ttatatagcc tagcctcaaa atcttttggg
     2821 ttataagttt ttgataaatt cgtattttcc attgtcctgc ctcctgtatt ttataaataa
     2881 attttttaaa tatctttaga aataaaaaag agctcttcat cctctttaaa aaggacgaaa
     2941 agctcgcgtt accaccttaa ttccaaagct taaaatacat taacatataa ttaaattatt
     3001 ggttaatgtt attttatcaa ctttgactct caaagaattt aacggttcta accgtctaaa
     3061 cttacttata gttcagttta gaaactccaa agctacattc agtaaatctt atacttagaa
     3121 aatcttacag ccagtgaatt ttcctctctt ttagtagtat ttacttactc ctcttcttct
     3181 cagtctttaa tattacatat tattatatac aaatattatt ctctttgtca attattttta
     3241 ttttattttt tctgaatttt cttaattatg tctattctct tttatatagt tgttatattt
     3301 taaaatatgt ttttacagtt attctgtctt ataatgtcaa taattgcaag attattttat
     3361 tttaatttta atgctttctc attaatttta tattatcctt acagtatacc tcttattcgt
     3421 attttttata ttatatgttt tattttataa ataatttact ttatctttta ctatatttaa
     3481 atttattata agttttcttg ccagaaattt ttaggtgtta tattttctgt aattggcaat
     3541 attgtatatc ctctctcttt taagattctt ataatattat ttaaagcatt taaactttgc
     3601 tctttctcat gaattaatac tactaattca tctctttttc ttccataata taatatatta
     3661 cttactatct gacttgtaga agacttccaa tcctgtgtat ccaaattcca gtcccaaatt
     3721 aaataatcat ttgccataag tttattatat gaaccttctg gcatatatgg tttacttccg
     3781 tatggtaatc ttattaattt tgaactctga ccagttattt cttgtagagt attattgcat
     3841 gtattaaatt ccccaactgt tacttctggg cttttatata acttatgtat atcatgactt
     3901 acactatgaa aacctattga gttttcttct tccaagactc tccttacttc ttccttgtgg
     3961 tttttcatat tctgattaag catgaaaaat gtcgctttga catcattttc ttttaaaata
     4021 tcaagtagtg catttgtata ttttgatgga ccatcatcta ttgttatgta tgctactttt
     4081 tcagaagtag cccctggacc ttttgtttct tcttgagatg ctttgtatat gatttctcct
     4141 aaagtattac tttgagagag cagttccagt gtattttcac cttttgaact tatattttca
     4201 actggaacat tattaatttt ttcctccaat aatgttccag ttaaaattaa aacagctata
     4261 ataaatatcc ttataaatac atgaaaaaca gccttattat ctttaatttt catcccccat
     4321 atctcccttt tataaaagca taagtttaag acttaaaaag ttatttttta tatttattct
     4381 ctttcttatt actttttata tctctactaa atattttttt aaccttttct tcttcttttt
     4441 ctcctaaaga ttctactctt ttgattccca aagaagttaa aaattgtaat acatttaaat
     4501 tatcaaatgg agtttccata ttcgtacttt cactatattc atcaacattc ttagtacctt
     4561 cttccacact taaaatacgc ttcggacctc ctacacagcc tcctacacac cccattcctt
     4621 ctataaatgt agcttctatt tctttattta gaactttctt tagaccgtcc atgcactctt
     4681 ttgttccttc aaatgccttt tcttcaaatt ttatattttt gtcaattctt ctaactgata
     4741 acttaactgc tttacttaca cctcctgttc tagcataaac tctacctgaa agggacgcct
     4801 ctactctatt actctcatcc attattgctg gatttaactc taatgcatca aatatttctt
     4861 gcaactcttt aaatgtaagc acaaaatcaa ctgcatcttt tatatcttct aacattgctt
     4921 ctttcttttt tgccatacaa ggacctataa atacaacttt agcatctgga tttaaaattt
     4981 ttacagctct cccacaagca atcataggag aaacggatgg tgatacattc tccaatattt
     5041 gaggaaaact attttgtatc aaactaaccc aaataggaca gcaacaacta gttataaagc
     5101 atccttcttc gtcctttttc atatgctcat aataatcata tgcttctttt gcagtaagca
     5161 tatctgctgc caaagcaact tctatcatat cttcaaatcc tatctttttt aatgcagttc
     5221 ttaatttacc agggcttaca tctttcccaa actgtcctac aaaagctggt gcaactattg
     5281 catatacagg gctctttata tcttttaaca attttatcat tggtataaat tctattttat
     5341 ctgaaattgc acccaactta catcttgata cacaatcacc acaagcacta catacatctt
     5401 cctcattaca attaatatgc ttacttctta tcttacatga ctcatcttcc ttattatttt
     5461 catcacaata tttgcaagca tctgatatta cagatattat tggtcttttg acttcatcta
     5521 aatttacagc ttcatgtagt gactctttta aatttttatc tgtatcttct gtcacatcca
     5581 atcccatagt taccctaatt aaatttacca tagtatcttt atcaaatttt ccactaaaat
     5641 aattatttaa attatccttt aaatcttcat ttaaaatttt attaggtacg ttttcaatat
     5701 cctctatatc atcattccac actgctttta caagttcgcc aaaaagcttc attttatata
     5761 tttctagtat tttagaattg tcatttctag tattcataaa aaatagtacc tccataaatt
     5821 aaacttatat atgtttaata tttacaaaga tactattttt tatttattat ttaactacta
     5881 ttattaatat gatttattta gctttctcaa caactctatt tcaatcatta ttttcattag
     5941 atgatagttg aatacagggc ttttttcttc atctatattc cagtctagct tatccacatt
     6001 atatgttttc ttaaggtatt tatatgtatg ctctttcaaa taaagtttat tttcaagtaa
     6061 ctctatagac tgcatatgaa aatgtatttc tctacatatc cttaaggcat tctcaatttt
     6121 ttctatatta gtttcactca aactataatc caactcatca ataagctttg aatatatttc
     6181 ttctaatttt tctacttctt cttcaaattt ctctacattt aaattttctt ttctaagaat
     6241 tttatcttca ataagcaaca ttgaaagttc ttctatcttt ttaaactcat ctataacact
     6301 atttagataa tttggtctag caacataata attaacaagt actccaaaca aaacacccac
     6361 agatgtatct aacaccctat gtaaagaata atacacaggg tcactatttc ctacacctag
     6421 atgtatagcc agaaaggtta cacaggcaac tactactgac tttattttaa acagattgca
     6481 taaatatatt gagcacatta ctcctagacc acataaaaat gggtctccag ctttaatgat
     6541 ggcaaatatg aatcctatta taccacctaa aatagtacct tccactctat tgattcccgt
     6601 ctttaaagaa ccttttactg tatcttgaac agaaattata catgctacca gagaataaaa
     6661 catattctct actaaatagt caccaagaat catacataaa gtgacactaa ttcctgtttt
     6721 tatagtcctc atacccatct tttgaagttt caaagttcca cctcctaaaa agcaccttat
     6781 ataaaatacg catcctcata tttaatatgt tcctagtata tcacaatttt ttaaatttac
     6841 ccattaatca tattttttta tcaattcttt cttaatttag acaataaaaa aactgcttta
     6901 aagcagtttt ttatcctatc tcaaccattt taccctcaat tgcaaaaatt atcttctcac
     6961 atacatttgt tatatggtct ccaattcttt caaggtatct tcctataagt agaagtttta
     7021 ccccttggtc tatggtattt gagttttcat gcattatttt taaacaatca atttgtactt
     7081 gttcatacaa tccatctaca atatcatctt tagttccaac atcataagct atacttgcat
     7141 caaataagat taaagcttct ctagtctgag acatcataga cttacattct tttgccatct
     7201 taggaatatc tattagctct tttatataag cctctccacc tatcttcaac atttctctag
     7261 ctatatttgc tgcataatct cctattcttt ccagctctat agatatatta cctaatgaat
     7321 aaataaatct taagtccctt gccattggtt gctttaatac tattagctct atactcttat
     7381 ctcttatttc ttctcttaat ttatttattt cttcatcatc ttctactact tttcttgcac
     7441 cctctaaatc cttctctatc atacaagtca cagacttttc tattgcttct tcacattttt
     7501 ccatcataag cactgtttgt tcttttaaag tatcaatact tatatctaag ctattattga
     7561 ccattgtcta actcctttat agttatatat tttaaccaaa tctacctgtt atatagtctt
     7621 ctgttctttt atctacagga gtggtaaaca tagtttttgt atcgctaaat tctataacct
     7681 ctccatttaa gaaaaaagct gtttcatctg atatacgagc agcttgttgc atattatgtg
     7741 taactatgac tattgtgtag tcttttttta aatcctctat aagttcctct acttttaatg
     7801 ttgaaattgg gtctaaagca gatgtaggtt catccataag aataacttct ggtctcatag
     7861 caattgctct tgctatacat atacgttgtt gttgaccacc agaaagtcct agtgcagatg
     7921 atttaagcct gtctttaacc tcatcccaaa tagctgcacc ttttaagctt tcttctacaa
     7981 ttttatctaa ttgcttctta tctcttagtc catgtgtacg aggaccatat gccacattat
     8041 catatatact cataggaaat ggatttggct tttgaaatac cattcctact ttagtacgaa
     8101 gatttataac atcatctgag gtatatatgt cctctccatc aacagaaatg tttcctttaa
     8161 ttgttacatc ttcgattaag tcattcattc tatttaacgt tcttataaaa gttgattttc
     8221 cgcatccaga tggacctatt aaagcagtaa ccttattctc ttttatatcc atatttattt
     8281 tctttaatgc ttgcttatca ccataaaata aatccaaatc ttttacactc atttttatct
     8341 tatcgattaa ttccattatt cacacctctt aaatttaata attagcttta tttaatttct
     8401 tagcaacaaa tcttgctagc atgtttagtg ctaatacaat tactattaaa acaattccta
     8461 tactggctgc tgttgctata tctccactct ctttagtcag taagtatgag tgtacagtta
     8521 atgttcttgc actatcaaat attccacctg gcatcttggc tacagtacct gctgtaagta
     8581 atattgcagc tgattctcca attatacgac caactgataa gattactcca gataaaattc
     8641 ctggtattgc acttggaagt actaccttat ataaagtttg aaattttgta gctcctaatg
     8701 ccaatgatga ttctctataa gcttgaggaa ctgtttttaa agcttcttct gttgtacgaa
     8761 ttattactgg taatattatt attgcaactg taagtgaacc tgcaactatg gaatatccca
     8821 tttttaatgt aactacaaag aaaattcctc caaataatcc atatactata gatggtatcc
     8881 ctgataaact ttcagttgca aatcttatag ctgtaactgc ttttcctttt ttagcatatt
     8941 cttgtaaata tatagcagcc aatattccta taggagttgc tacaactata gaaactacta
     9001 ctgtatataa tgttgtaatt atcattggaa gtataccacc atcacctgat gctgaataat
     9061 tgctaaataa gaaattaaaa ttcatgttgc caattccctt tataaatata aatcccacta
     9121 taactactaa tgatactatt gtaaatagtg ctgatagata tactaatgac ttcaatagat
     9181 tctctttgaa ctttctcatc ttaatttacc gccttatttg aaagtttatt taaaactaag
     9241 tttaaaatta atataaatgt aaacaatact actccagttg caaatagcat ttcttgatgt
     9301 gtaccaaatg cgtatcccat ttcaagtgct atattagttg taagtggtct tacactatct
     9361 gttattgcag ttggcataac tggtgagttg cctgcaacaa gtattactgc cattgtttca
     9421 cctaaagctc ttcctactcc taacactact gcggctaata ctccagattt tgcagcaggt
     9481 aatacaactt tgaatattgt ttctattttt gatgcaccaa gtgctaatga accttctttg
     9541 tatgcttttg gtactgctct tattgctgtt tctgatacag atattattgt tggtaacatc
     9601 attactgcta atactattac tattgctaac aaactttgac cttttggaag attaaatata
     9661 ctttgtatag ttggaactat cactgcaaga ccaaatacac catataatac tgaaggtata
     9721 cctgctaaaa gttcaactgc tggtgacatt atttttgcta atcttttagg tgctacttct
     9781 gctataaata cagaggttaa tataccaaca ggtactccta taacaagtgc tccaagtgtt
     9841 gcaaatattg atgctactat catagatgat attccaaatt tattggcaga aggaacccaa
     9901 tctgcaccag ttaaaaagtc tataaatgaa tatccttcaa atataaatgg tcttaatcct
     9961 ttataaaata cgaatcctat tatcaaaagt aaacttgcaa ctgctactaa agcactaagt
    10021 agaaaaatat tttttgctac tttttcaatt acgtattttg tcttattgcc attattgtca
    10081 tttttttcta aacctactaa tctagatttt actatcataa taccctccca ttaaaatata
    10141 cttatcgatt ttcgttctgt attcaaaatt taatttttta taattcgtct tcatcaaatt
    10201 tcctatactt ttattctact ttttttaagt taaatctata ttaggattaa gttaagcaag
    10261 tgtaaaatta tatttacttt ttaacattaa ttttactttt aaaactatat ttgacattta
    10321 ctttacaata tactgttgtt tctctatatt aaaatagcag atatagataa taatacagtt
    10381 tatgtataat ttattaccta tatctgctat taagatttgt taactaaatt taacttttat
    10441 actacatata taattattgt atcctctagt ttatatcact attatttcca tcaaattgtt
    10501 acgatacttg atgtttgcaa taggtacgaa tagccaaaaa tataaaaata ggtatttcta
    10561 tcaccgagaa aaacagtata atgtgtggtg tccaaaaact catacctcct aagtgtaaga
    10621 aattaaatga tgtcatttga tataataaac taatcttaaa accaattccc cctggtggta
    10681 ataggtatcc aatccaacta acacctattc ccataggaat taagcaaata gaaaaagcaa
    10741 tcagcataga agtaagagaa tccttacatt ttgcagataa aaataatgtt aaactaacca
    10801 ttgcaagtat tgaaattaga ccaccaatag ctataagaat ctgtgcttgt agtaaattta
    10861 tatttactag actaatgaca gaaaacaaca tttgaaatga ggttttcaaa cattcagtac
    10921 caaaagctaa atcagaaatt gccaaataga tagaaataca tataacaaac attattataa
    10981 atatagaaaa tagagctaat attttagtaa acgctagacg aatatgtcca tgtttcgtac
    11041 aacgaaggat gctatctgat tgtgtctgat actcaccaga aaaagtaggt gcagcaattg
    11101 caacatatat aattaataat ataaaaatat aaaatattat ataatcgaaa acatcttctg
    11161 gatttattaa gaaattctta atcttattat ttaactattt tattatttaa tatatattaa
    11221 aggtaaaaaa ataatatata gatttagata tacaatttat ctatcttttc aaatataatc
    11281 tataaaataa tagttccatt acgcattttt ttatataata aaactgactg taatttaact
    11341 ggcaaagtct agaagaatag gctttatcaa ttaaattaca gtcagctatt ttataataat
    11401 ttagtttaga tattgttagt atttcacatt tataataatt attaatgata ttaatatatt
    11461 tattattctt tattaaaata atatcctgct cctcttattg ttataatttt ctgaggattt
    11521 ttataatctt tctctatttt ctttcttaat ccattcactg caactaccac actatagtta
    11581 tcctctaagt attcattcat ccatacatgt tcatatatct ctttagtagt caaagtcttt
    11641 tcaggattct ctataaaaaa gtttaacaac ttattttctg ttggagtcaa ttttattatt
    11701 ttatcttcta actttattac tccactagtt ctattatatt cctctaattt tgtattattt
    11761 tcattattat ttacatatga gttcattctt cttaaaatag cttttacttt tgcgtataat
    11821 atttctaaat ttagaggctt tattaagtaa tcgtctccac cagaatttaa agcatttaaa
    11881 atgctttggt cttcatttat ataagtcata taaacaattg gacaagtagt tacatttcta
    11941 atttttttac aaagagtcca tccatcacca tcacttagaa ttatttctaa aaatattaaa
    12001 tcatatttat tgctaaatat ttttccaata gcttcttcac aattatatgc taaatctata
    12061 cttattcctt tttcctccag aaatcctttt agcttcttgc aattatcctt gtctccatct
    12121 attatcaata tcttagtttg cattataatc actcccaaat atcattattc ttttatatta
    12181 aataagctat tattataaaa aacatagact taattttatt gtaaaaacag ttccttttcc
    12241 tagttctgaa tctacaaaga tttctccacc atgctctctt ataatggatt tgacaatata
    12301 tagccctaac ccttgccctt ttggctcatt attgtctctt tctgtaaaga atctgtcaaa
    12361 tacctttggt atattttctg gaggtatacc ttttccatta tcagaaattt taataattgc
    12421 aaatccatct tctttatcca ttgataaaat tatctttcca tcaaatggag taaaggtcaa
    12481 agcattataa attaaattct caaatgctct tgtaagcatc tctttattta tctttgcata
    12541 tatttttttc tccaatacgt aagtttcaaa gtttacacca catgcttgtg tatctggcaa
    12601 attattttca tatatctctt taataaaatt attcagacat attttttcac taataccaat
    12661 gcttttatct tgtgatgtaa attcttgcaa actacgtact ctattttgta cttcatttga
    12721 cttttgattt atagcataaa gataggttct tgtctcttca tctacatgaa tattaccaac
    12781 ctttatcaaa tctataaatg cttgtatagc cgaaacaggt gcctttaaat catgtgctac
    12841 atctgccaag aatcttttac gttcatttaa caatgttgta agttcattag ttctcacttc
    12901 aacttcatgt tcaagattcc ttgtaagttt ttcattctct tttattatta ttctattcct
    12961 gtttatcatc ataacagaaa ataaaataac aattacaaac ccacaatatt ctgtttgcca
    13021 ccctgtaaat ataggctcaa atctattact cgtaactgca tcaaataata tgctaattcc
    13081 aaaaacagtg tttactgaaa ctaaaatata agaacttatg tgctcagagc atgtcttttt
    13141 atatattgaa accagcgaag acattaatat atatataaat acaatatatt tatatatgtc
    13201 tattaacttc ccatatatgt tgacaaagta catatcatat ggaagaagaa taattggtat
    13261 cactatagat atgatactca ttgtaattcc taatggtaaa attaaaaatc tgtacacttt
    13321 attttcttct attttggata tacttccatt tatacaaata atacataaaa ctaccataaa
    13381 atatgacaaa tcttcaaccg catatgtaaa gcttaccaaa ggcagtccta acaaatgtac
    13441 aaagggatag gatacatgga tggcaaagaa aacacacata catccaaaat ataagtaaat
    13501 tttacttttg tttgaaagaa accatacgga caatgaaaat atagctattg caaaagtcgt
    13561 gaagcaaatt atactataaa aaagtaatcg ataaaacatc atattactta tgtcctttgt
    13621 ttctcctaat gcaggcggat aatacaaacc actataataa tgtgaaaaat tcgatacatt
    13681 gattataatt tctgtatttt tctttgcaat aaaacttact actgaatttt taattttggg
    13741 tctatatttt tctgttccta catctcctaa tttagaaacc atttctccat ttatccatac
    13801 ttcacttgcg gaaaaaatct caggaagctc taaacttagt agcttatcct tgccttcatt
    13861 ttttattctc atacgataag tagcattccc atatggtgac tgattcccct ttctcataga
    13921 aaaatctgga tattgaccta taaaaacata ttctggtttt atatcttgct ctgtaaaatc
    13981 attcggttta aatattttat gattataaaa ctcccagtca tctatcaaaa agacaagttt
    14041 acctttagtg aaagtatcat tatctatagt taaaattcca ttacttgctc taatagactt
    14101 atatgtatat ttattatcat atttatacag aagatttaat cctgttattg ccaacagaga
    14161 gactatcaat acaatgagag ttccttttag tatattatac tttttcattg catcaaccac
    14221 atctctttca tagtcgtatt aaataaaaca agaactattt attaattaca gtatctcact
    14281 tatggtacaa acttatatcc ttttccccat acggtttgaa tgtattgatg ctccctgctt
    14341 aaagaattaa ttttttttct catctgtgca atatgaacct gcactgtatg ttgatctcca
    14401 atatcaggca ttttccaaac ttggttatat atctctatct tcgaaaatac ttgttctttg
    14461 ttttcagcta gaaacaataa tatttcaaat tccatagtag ttaaatctat actttctcca
    14521 tcaaaagtta cttttcttga atttacattt attactaacg ctgaaaactt taacacatta
    14581 ggaactgatg taatattttt gtactgcctc atacgagcat atattctaag ttcaagttct
    14641 tttaaactat aaggttttgt tatataatca tcccccccag ctaaaaatcc ccgaactttt
    14701 tcttcttcat gctcataatt agataaaaat attataggta tgttaacctt ttcttttata
    14761 tcattgcaca ttttatatcc atctatatct ggcaatgaaa tatccaaaat tatacagtct
    14821 aaaactatac tctctactat atccatagca tctttagcag tctctgatgt atatacatta
    14881 aaacctatat tagaaaaata aatttgattt atgtttaaaa tatcaacatc atcatctaca
    14941 accagtaaat tatacattac accactccat tcaaaggtat tttaattata attccaagtt
    15001 ttatagttat tacaaattat aatgttttat tttcatatat aactggtatt attattttat
    15061 aatatcttta caaactagac tataatactt aagtattttt gaataattct tgaatattgt
    15121 attaaacatt aagattcatt attaaatact aattaaagtc tccgattgtt ggagaatgga
    15181 gcttaaagtc attttcacgt ataaatttat tatttttcta gaaacttgtc tattggctag
    15241 tttgagtatt gatttatatg atttatatgg ttataatata aaataaattg tattattttg
    15301 gtaaatatat tgttacaaaa ggtttatatt ctgcatctca atgtttataa aaactttatt
    15361 ttcttattat aatactatta ttcttttaaa aaaatttccc ttttccaaca aatggctact
    15421 ttaaaagcca aaattttacc tatcaataaa aatcttaacg actaatactc ttattttcaa
    15481 attttgcaac atatttgtta attttaaaac aaaaaaagac tatgcaatat aaaaattata
    15541 ttcatagccg gttgcaccac taactccata tatctctaga tgcccaataa taagatatac
    15601 ttctcagttt ctaaaatatt tttattaaaa atcttttata tattttcata aatttctttt
    15661 ttattctaac attgtaatat tattttgtca atatttccat attcttagat ataagatact
    15721 aaaataccta ttttatctaa agatatttat atttctacat tcaataatag acaataccct
    15781 gcaagttaaa gcatcatttt tatgagctta tctcacaggg tattatatgt ttactttata
    15841 ttataactct tgttattgat taactttaat aaatcctttt tctgaaacaa gtgcttgtcc
    15901 ttcatcactt aatatataat ctattaaagc tttacctttt tctgacaaac catcttcttt
    15961 attaactaat ataaatggtc ttgatattgg atatttatta gctattacat ttgcatcagt
    16021 tgcttcaaca tcatttattt ttaaactatt tactttctca tcaacatatc caaatgatat
    16081 atatcctata gcattttcat ttccttcaac tgtagttttt atatttcctg aaccatcact
    16141 tatttgtgca tctttagtta gttcttctga atcaaaccct acaatttctt ggaatccatc
    16201 tctagttcct gaaccttctt ctcttgatac tattactatt ggtgcatcat ttcctcctac
    16261 ttctttccag ttagtaatct ttcctgtata tataccttta acttggtcca ttgtcagatt
    16321 ttttactgag tttgcagtat tagttatcaa tgctatacca tctattgcta ttttagtttc
    16381 ctttaatcca gcactttttt cttcatcttt taaatctctt gaactcattc ctatttcaga
    16441 tgttccttct atagcatttt ttatacctcc agaagaacca atctgttgta cttctacact
    16501 aacaccttga tttttttctt gaaacttttc tccgatagtt tccataagag gacctattga
    16561 agttgaacct gaaacagaaa ctttgcttga atctcctgaa tcacctgaac ctcctgagtt
    16621 agaactacat cctactgcta aagctccaac taacattgta ctagctaaaa gaacgcctat
    16681 tttcttttta aacattttac ttacccccgc ttatatttta attttttgtt gtcatttttc
    16741 tatattttaa taatacagat tctttgttaa atgaatatta gagcagggtt aagtaaatgt
    16801 aaatattgtt aaatacatgt aaaaacttaa ttttatatga tttattctta attagattaa
    16861 ctttttaaag ttactttagg cagatataaa cataatacaa ctcatattat cagaagtctt
    16921 cattgaagct atcaacctat cacatcctat aattcttttt ctagtttatt aactaattca
    16981 gcaaatacat ttaaggcttc ctctatagat tcttttttcg tcatatctac accagcagat
    17041 tttaattgtt ctagtgggta ttctgaccct ccacttttta agaagtttat atatctatct
    17101 actgcagtac ttccttctga aagtatctgt tgacttaaag cacttgcagc agaaaaccct
    17161 gtggcatact tatatacata gaagtttgaa taaaaatgag gtattctcgc ccattctatt
    17221 cctatttgtt tatctactat tgcagatttt ccgtaatact tttcatttaa tttgtagtat
    17281 atattagtaa attcatcagc agtaagtggt tctccactct ctaccctttg atgtacaatt
    17341 ttttcaaatt cagcaaacat agtttgtcta tgaactgtag ttctaaattg ttctaaataa
    17401 taatttaata agtatatagt ttcatcctta gacttagaat tttctaataa ataatttata
    17461 agaagtaatt catttaatgt agatgcaact tctgcaacga atatcttata gtccgaatat
    17521 aaaaatggtt gacttgttct tgaataatga ctgtgaacag agtgccctaa ttcatgaatt
    17581 aaagtgaata gagaattaag gtcattttta tagctcatta atatataagg atgtgaatca
    17641 taacttcccc aagaataagc tcctcctttt ttaccttcat tatcatatac atcaatccac
    17701 ccttcatcaa aagcactttt tatgaccttc aagtattctt ctcctaaagg tttaagtgcc
    17761 tctaatatga tatcctgtgc tttatcatat ggtatatcca tgtcaaattt atctgtcaaa
    17821 ggaacataca aatcatacat gtgtatttca ttcaatccta aaaatttctt tttaagctca
    17881 acatatttat ttagattagg aatactttta tctatagctg atataagatt attgtaaaca
    17941 tctaaactta catcatctga aaataaagac gcttctattg ctgaattata ctttctagtt
    18001 tttgcgtaaa atatctctga ttttattcca ccataaagcg ttgaggcaaa tgtattttta
    18061 tatttctcat atgttgaaaa ttcaccttca aaagcgtctt ttctaactct actattctta
    18121 ctttttataa ataaagagta gtttgaatga gaaagcttaa ccttattccc ctcctcatct
    18181 tctatctcag gaaattccat atctgcatat gctaacatat cgtatacatt ttctggaatt
    18241 gtcgataatt ctgatgtagc tgcaagtatt tcttcctctc gttcactcaa agtatgtggt
    18301 ttatctctca atatatcctt tatatatttt gtataaaagg atagcttctc atcttttaag
    18361 tattcactta acttattctc ttctatcgcc agtatttctg gaactatata agaagtagcc
    18421 acacctaaat cagtagataa catttctgtc ttagtggctc ttccttgatt atcattaatt
    18481 ctagtatctt catgttgttt catatgagta tatacatata aattttgaac aatcctagat
    18541 gcattttcag agatatttaa tactttatat aagttctcct tactctcaga aagcttccct
    18601 ttatattctt ttacttcttg gataagattt tttactttac ttatgtcttt ttcaatttcc
    18661 tctttactgg agtacatctt atctatcttc catttgtact tttcttctat tgtacttcta
    18721 tcagttccca tatcatacac ctctatttta atttgtttta tattttattc actttacaaa
    18781 atatattgta tatattttag tccaaataat ctacatttat tactccttct atcatattta
    18841 tttctttcat aacgtcgtgt aagtgtattc tcttagcata agatacaaaa aaattaacct
    18901 tccagtattc ttcattacga cattctattt ccatatttct tacgataagt tgattttttt
    18961 caaatttatc atataaaaaa ggtatagaat ccaaattgct taattttaat atcaatacat
    19021 tcttttcttg gctaaataaa aacgtattga ttatcctaag cacaaataat gctatcatta
    19081 caaataatcc acctagaatt gccaattcat aataaccata tccaactgct ataccaatac
    19141 atgcagttgc ccacatccca gcagctgtag taagcccttt tactccacct ttatttttta
    19201 aaattgctcc tgcacctaaa aaaccaattc cagataatac ttgagcaggc attcttgctg
    19261 ggtcagcatt tgtatacaaa ttataatcaa agaataattg aatcgaagtt attgaaacaa
    19321 tacaagaacc tattgcaatt agtatatgtg tcctaaatcc tgcaaactgt cttatttttt
    19381 ctctctctaa gcctattatt cctcctatta ttagtgctaa tagtaacctt attgctatct
    19441 ccttacttcc tatatcaatc ataatataaa accctcctga cttaattaca acttatgtcc
    19501 tacagataat ttacacatca gaaatactat tttatatctt aatattatta ttaatatatg
    19561 taaaatatag ccatctgtta atttttgata caaaaaaacc cacaactgtg ggctatttgc
    19621 ttaggtacat gtatttattt attaatatat ctattttctt acttgattct actaactctt
    19681 cttgaaatat ttctggatat tctatgtatg tatttatctc ttccctaact gcttcaatct
    19741 cattttttaa cgcttctaat tcattgttcg ccataataat caccactatt acaaatttgt
    19801 atatttttat atacatttta tcaaacaata ttatattttg tcaatattat gtcaataaaa
    19861 cttactataa aaatgcttta atttaggtat tgtatacctg gatactaagc ctacaaacat
    19921 accagttact aatgatgcca ttaacataaa tggtaagtac cctatagcaa gtatattttc
    19981 tatgattaaa cttgcaacta ttaattgacc tatattatgc cctattgcac ctataatgct
    20041 tatccctatt agtgataaat cttttatttt actcgctaaa tacataaata aaagactcag
    20101 ataagctcct cctatactaa atagtagata tgataaaggg cctgcaaata ttgatgacaa
    20161 aattaccctg atagtaacaa ttataacagt atctttgaca cctaacaaca ttaatgaaat
    20221 taaagttata atattgctca aacccaattt tgcccctgga aatataacta taagaggatt
    20281 tggtatatat gtctctaata catataaagc taaactatac cctatcataa gacctaaaaa
    20341 tgtcattttt ctagttttca ttaagttaac tcctccaaat tttatctatc atcttacatt
    20401 ataatacaaa atatataaaa acacctaatt tagactaaaa tatttttaat ctatttagaa
    20461 taaatattag ttatttcaat gtgcaattac ctctataaat cttgactttt tgtatcatac
    20521 ttgttacaat tttaatgata atgaatttca ttaggaggtg ttaataatga ctgtttacga
    20581 tttaaaaata ggagaaaaag gatttattaa tagtatagat ggagatatta aattatctaa
    20641 aagattgctt gcacttggat gtataaatag tactgaaata gaagttaaaa aggttgcccc
    20701 acttggagac ccaattataa ttagatttag aggctttgac cttgcgatta gaaaatctga
    20761 tgctaaaaat atttctttac aataatagag gaggagctac ccgtgattaa tgtagcatta
    20821 ttaggaaacc caaatgtcgg taaaactact gtttttaatc tacttacagg ttcaaatcaa
    20881 tatgtaggta actggcctgg tgttactata gaaaaaaaag aaggatttct tggaaaagag
    20941 ataaaggttg tcgatttacc tggtatatat gcaatggata ctttctcaaa tgaagaaaag
    21001 gtttctaaat catatttaga aaatgaagat gttgatgtaa tagtaaatgt agttgatgct
    21061 tctaatttat caagaaacct ttacttaaca actcaactta tgcaatttaa caagcctata
    21121 gttatattgc ttaatatgtt agacattgca gaatctaaag gagttaatat agatgccaaa
    21181 aaattaagtg aagaacttgg agtaattgtt gttccaatta tagcaaaaaa gaaaaatggt
    21241 gttgatagga tagaagaaga gattaaggga gctacgcaaa aagcttttgt atatcagaaa
    21301 aactttggaa gtgaaacaga aacatataaa aaattagaat ccattttatc gagatgtaca
    21361 aaaactacat atacacaaaa gaaatctatc agtgataaga tagacaatat agtcttgaat
    21421 cctatattag catatccgat atttattggt gtattgttcc tcttatttaa atttaccttt
    21481 gactgggtcg gaggaccact acaagaggga tttgctagtc ttatagaggc ttatatttct
    21541 gcacctgtta gtgatatgtt atctaactct agcccatggt ttaaatcact tatagttgat
    21601 ggaataattg gaggtgttgg tggtacttta ccattcttcc ctttaatatt tacattattc
    21661 tttggtatat cgttgtttga agatagtgga tacatgtcaa gaacagcatt tttaatggat
    21721 aaagttatga gaaaggttgg tctatctggt aaagctttta tacctatagt tatgggtatg
    21781 ggatgttctt ctccagctat aatggcaact agaacattgg aaagcgaaaa agatagaaga
    21841 gttacagctc ttattgcacc acttatgact tgtggagcaa agctacctat atatgcacta
    21901 tttgtagcta tattctttcc acataatgct gctttagtaa ctacatcact gtatttggtg
    21961 ggtatagtta tggcaattgt agttgcatta ttcttaaata aaacagcctt taaaacagag
    22021 gctgaaccat ttatattaga attaccagaa tatagaatcc ctactataga tgcacttatt
    22081 aaaaatactt ggaataaatc aaaaggtttc ttgataagag ttgttactgt catgtttgca
    22141 atgtcagtcg taatttgggg actttcttca ttcaatgttt ttggatatac agaagatata
    22201 aatgtaagct tcttggctac tctaggtcat attatatctc ctatattcaa acctcttggt
    22261 tttgatgatt ggagaacttc agttgctatt ttaagtggtc ttggagcaaa agaaatcgta
    22321 gtaaactctt taaatatact atatggtaat ctaactgttg tcttaccaac aatatttaat
    22381 ggagttactg catatacatt cttgatattt actgctttat atactccatg tatagctgca
    22441 cttgcaacta tgaaaaaaga atatggcaat aaaatgatgt ttacatcatt tgcatatcaa
    22501 tttatactag catggataat ggcttttata gttaaaagta taggtggagt gctatttatg
    22561 ggtaattcaa taatggaagc tttgataggt ggaataatta tagtccttgc actctttatt
    22621 ctgtttaata aattcaagtc tacaaaaaac ggaaatgtct gtagtggatg ttctatttgt
    22681 tctggatgtc caagtgctaa ttcttgctca agtaaggaag acagcaataa aaaaaataat
    22741 gcacactaat atataaatat tttagtttaa aataagataa aagtgggtgt ctcaaaatta
    22801 actttttagt tcatgaggca ctctttttta tagaaaatca aatatatttt catcatttca
    22861 acagtaaaaa agagcttatc tagtaaaaaa gaggcttatc tctattttga gacaacctct
    22921 ttttttgtta cttattcagt ttcttgctac ctaacttcat gtggaaccta gtatcttttg
    22981 gaacacttgg gtctacacct cttaatgtag gtatttcccc acatattagt tgtataggta
    23041 ctataaaatt aaatgtttga tagtaaggat tatctgtgat atcaatcttt aaatttttat
    23101 cattttctgt aacatctcta cctatagcaa atacattttc cgtccaacca gaaagtacat
    23161 caatcatctt agttactcta ggttcttttc ctgtatctag tataaatata gtagaatcac
    23221 tatttatagc attatatata ccatgtataa actcttcaaa ctcataccca gttacaggta
    23281 ttctcattgt ttccaataat tttaatgctg cttctaatgt atctccatat atatcagagt
    23341 gacctattat tcttatttct ttagaattaa ctaacttttc tttgtttctt tcaatccatt
    23401 gtttagagag cttatacact gcttcaaatc tattaattgc atctaatatc ttatttattt
    23461 cctcattgta cttttcacta gatattatac ctttttctct agctatttga agacctaata
    23521 acattaagtt taactttgta caataatatc cttttgtttt agctccagat ttttcttcac
    23581 cacaatttac tgttaatata taatcagaaa tctcatctat taaagcattc ttacaacctg
    23641 ccatagatgc tattttacag cccttatctt cagctaactt catagcgttg tatgtagaat
    23701 aactactccc accttgagat acacctacta ctagggtatt ttcattatca aacttgaatg
    23761 tatcttcagt tatcataaat ggatacattt tcacaacatc tatatctaat aaattttgta
    23821 aatatggttg cacttgcact cctgagtgat agctagtacc tgaacctgtt attataatct
    23881 tttttagatt tgttctctta acttcattaa ataagctatc tgcattagat ataatttctc
    23941 tcattctaac tggtgtttct aacatataat cttgtattgt cattttatac accttcctta
    24001 tctatatatt caataaatta taattaattt ctataacaaa cctatagcat gtaaagcgat
    24061 tcctgttact atagtgccta tagttaattt tgttacactt acctttctct ttaacatgta
    24121 gtatattaca aatgcaaagg ttaaaggtaa catattaggt attattttat ctagtatatc
    24181 ttgtaaaatt acttctgcac catttaagtt gaatttcaga ggagtagtta tactcaacat
    24241 agttgcaacc attcctccta caacgaataa accaacagtt gtagccatag acattatttt
    24301 ctccataagt ccctctcttt gtattctttc aagagaattt acccctactt gataaccata
    24361 cttaagtcca aatattctaa gggcaaatgc tggtatattg tataatagta agaataatag
    24421 tggtccaaag atattacctt cttttgcaag tgatactcct actcctgctg ctataatcct
    24481 aaatgttccc cagaataatg aatcaccaat tcctgcaaga ggtcccataa gagctgtttt
    24541 aacagcacta attgaacttt catcaaattc tggattatta gcattctctt cttccatagc
    24601 tgctgttata cctaatatag caggaactac tactgtcgtt gtattaaaaa tctccaaatg
    24661 cctttctatt gcttttgctt gattttcttt cttattgtat agcttcttta ttgcaggtaa
    24721 cattgaataa cagtaaccta agttttgcat tctttcgtaa ttaaatgcac cttgaagagc
    24781 aaatgaacgc caaaacatac ttcttaattc tttttttgtt aatttattca aagtcaatct
    24841 ctccttccgt taaagtattg attgctaatt gaggattttg attattttct ttgttattga
    24901 tataaaagtt tagtacaaat gctacacata ctccaaataa agctatagca gttatatcta
    24961 atccaccata agatgctaac aaaaacccta taaagaaaaa tacagacatc ttttttgaaa
    25021 gtaacatatc cataagcaat gcaaaaccta ctgctggcaa caatgttcct gcaacaccca
    25081 gtccgtctaa tataacttta ggtattgcat ttattaatgc tgtaacttta tcagcaccaa
    25141 tcataattgc taaaaaggtt ggaactgatg tacttaaaaa gaataatatt actggcaacc
    25201 acatcattat ggtaactttc ttaaaatcag cttgtttagc atagaaaact gccttttgag
    25261 aaaagtacga atttattatt ctaactaata ctccaagaga ttgtgctaag actgctactg
    25321 gtactgctac tgctaaagca acttctgcac cattacctga taatatagca aaagctgtac
    25381 ctaatacacc tcctgttact acatctggag gagtagctgc acctattcct gcaattccca
    25441 tccaaattaa ttcaagctgt gcaccaatca taattcctga ttttatgtct cctaaaacta
    25501 atcctactaa catacctgta actattggcc tatcaagcat agattgacca aatatacgcc
    25561 catctagtat accaactcct gctataatag ctaacatagt agcttgtact aacatattga
    25621 acctccattt taacttagtt taatttacat taaattacat tacatttttg attttgtttt
    25681 gattttctaa taactcattt acataaacaa ttccattttt tgcttcctct aatcctgatt
    25741 taactattag ttttgtatcc tcctcaacta ataaaagtat agataaaagc attggtaaat
    25801 ttactcctgt caataattca aatttgaacc cttgtaaaag taagtttgta catatgtttg
    25861 ccacacttcc accaaacaca tctgttaaga caacaaattc ttcactatct ttattatttg
    25921 ataatatact tctaatttct ttctcagcat catctgctga tttatccgtt gtcatgcaaa
    25981 aatactcaat attagttaat gaaccaatta ttaatttact tgtgtcaaca agctctctgg
    26041 acaattctcc atgtgttgca attagaaatt ttttcattct ctttctccta aattatttta
    26101 ttaaatctga tagttgtttc tttttgtctc taggtaacat tctaaattct atatcaatcc
    26161 ctaaatcacc tatttgttta aagttattta tatcatcatc atcaacagca actaaatctg
    26221 taagtttctt cttcccttcc tcatatctta atcctcctac atttatttct tttaatactc
    26281 ctcctgaatt tttagctaca actaaagcat ctttggtatt ttttactaac actaatacat
    26341 tgcttttctt tgaagaagca aatttttcta ctatttctcc agattcctct acccccctta
    26401 tatataaagt tacattgcta ggtttcgcta agttcagtga catagctttc atttggtcat
    26461 tttttgcttc atcatttgca actagtatta catttatacc tagtgatgtt gcccatccat
    26521 atgcaacctg accatgtatc aatctgtcat caattctcat caaagatacc attttcccca
    26581 aatcctccat catacattaa aatttttatt gtttatactc tcatttatct ataaaagcaa
    26641 aaagtatgcc aaatactggc atactttttt aaatttcatt cacttatttt gtaatcttgt
    26701 attttactgt ggtagaccat agataatatt ataaatatat cctatctctg aaattggtat
    26761 ttgcacacta taactctttt ctagcacact aaatgcattt tttattagtt ttacttcact
    26821 atgatggttt ttgattaaca aatccatatc agtatactct tcaatttcag tttgtctaac
    26881 taatctttca atcatacaac ttaagtgaac atataaagaa atctttttat catttgaaag
    26941 ttttttcttt tgaatatttt ctatagcatt aattccttct tctattctct ctattatctt
    27001 ttttgaatct aaaatagtta gactatctat tactctaatt aatgaaaaat tctttacaat
    27061 agaatcattt atgttcttta ttttttcatt ttctactact ctgctaaata ttctaaaaac
    27121 atcatcttct ccttttcctg atattatatc ctctaataat ataaaatcta ccccattcac
    27181 atttggattg gcagttccta ctatagccat cacatcatac atttcaaaaa ctggttcttt
    27241 atctcccata gaaacaagac tatcataatc ataaggtatt attttgacat caactattcc
    27301 aattaaactt tcggaaagta gatgttggat ttttatagca gtacctatcc ctgtaaaaca
    27361 agatgatatt atagcatatt ctttattttt ttgaggataa tacaattgta tattaggttt
    27421 agtttcttca tccataattt ctgcaatctc ttcaatgttt ttaccttgca ttaataaatt
    27481 tcctacttct agtgcagaaa tagtggatat ttggtctata aataacattg ggctatttat
    27541 cttattcttt aaatcttcat atataagatt aagagagccc atatcaaata atacaattaa
    27601 accattcttt atcctatctc cctctaggta tctagacatt tgttttgata catcagcaat
    27661 agtagcatct ataggcatat caatagctgt aaatatattt ttacccaaca tccgattaca
    27721 tacattcgcc atactactgg cagtagcata tccatgtgct ataatagtcg cattaacttg
    27781 attaaatttc attcttataa ttgttgattt aataaatata atcattatga ttgtatctac
    27841 attattaaga tacatatcca atctattcgt tattaatgtt gatattttac ttattataga
    27901 gtattctttg taactctctt tttttaaata tattagtaat ttattttcta aagctttata
    27961 ctcatcagac aattgataag attcgtcctt caaataaaga taagacgaaa atgccaatac
    28021 attatcccca tcatatttta tattgttatt aacttctaaa tttctaaata tatcctgcat
    28081 tatcgatatt ataagctcca taagtacgtt attattttca tcattatttg aaaagactaa
    28141 attgtccatt agtctaatca ttagattaga actcttttta ctaaattttt cctttgttat
    28201 ttcatttttt tctagtctat caaagtaatc taaacatttt atgaaacttt gatagattat
    28261 atttctttta tttcttccat ttgacatttc aatactaaat tgtttaggag aaaagataat
    28321 gcttttgctt ttaccacttt ggaattgaaa tatatctaca ttttctatat aaagatttct
    28381 aggaaggtct tgaagagtaa caaaaatatt tccatttctt ctttgatttt ttgcataagc
    28441 tcgtccacat gcatatttta ttacattttt acattcacct atattcccct tgaattttat
    28501 ctttaaaatc gcatttaatg taatttgagt tatctctata ggcaaaccaa gtaccttact
    28561 ttcttcataa ataaaatgtt ctacaatctt tcttttttca atagtccctc tttcttctat
    28621 attgggaaca taaatattta tgggtattct tcttaaaaag gtttgtaaaa attcagatag
    28681 actttctgtt gtagcaaata ttaatcttac cctagctttt ctaattactc cattctctcc
    28741 aatcctagaa aacgtacctg tatccataaa tgtaaataac ttttcctgac cttcactatt
    28801 taatctatgt acttcatcta aaaataaaac accaccatct gattgttcta ttagacctgc
    28861 tctagattca tttgctccag tatatgctcc ttttacatgc ccaaataata gactagacaa
    28921 taactccaaa ttattataat actgcgcaca gtttaacaca ttgtatggag aatttttttc
    28981 taatacatta ctgcttacac tatatttgta tattatcttg gctaaaaaag tttttcctac
    29041 tccactttca cctgtcagca ttattgtaag tccgccattt ggataaagta cagcggtttt
    29101 tatttgttcg attacttctt ttaagctatt atcatatcct atgatactat taaacacagt
    29161 ctcttgcttt aataatttac actcattttt aagttcatct aaattttcat aaatactttt
    29221 ttttgtaggt cctactacat catttatttt atttttaggt aaaaaaataa caggtctact
    29281 gtttatcttt ataagattat cagattttac taaatcattt aataatctac ttgattgagt
    29341 tctacttaaa ttaaactttt ttactatatc ttctgttatt atgccacttt cttttttaag
    29401 ttcatcaaag ttgttaaata atacacattc atttacaaaa cttaaaattt catccctcat
    29461 gttcaacctc caatacaaat atcaataata ctataatatt agtatatcat ttatatattt
    29521 atgaaaaaga taattatatt cctatcttga ttctcttttg taaggttgta aaatcaaact
    29581 ttttataagt ataaaatcta gattttttaa cataatctca aggttcttat ctttccatat
    29641 atctgacata gaatttatat aagaatatat ttattgtatt tattataata aaaagtaaaa
    29701 cattataggt attttaaaac ttattagcga agaaaatatt tgtaactttt attaccttat
    29761 gaatattatt tcaaatttaa aaaggctata a
//
LOCUS       DBGJFF010000038        29586 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.038, whole genome shotgun sequence.
ACCESSION   DBGJFF010000038 DBGJFF010000000
VERSION     DBGJFF010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29586
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.038"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(150..2120)
                     /gene="ftsH"
                     /locus_tag="ACOIAF_001729"
     CDS             complement(150..2120)
                     /gene="ftsH"
                     /locus_tag="ACOIAF_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HGZ2668743.1"
                     /translation="MNKLLKGAGFYLLVFIIIVGIVQFSGKPTEKIKDLKFSEVYREL
                     TDENISRLYFVNQTSVEGTIKDTNTKFKSYVPTEVMGNKLADEVLDQAKAGKLTFGGE
                     AKPSTPWFVEMLPTLLLIFFMVIIWFVFMNQSQGGGGKVMSFGKSKAKVHKDDEKTRV
                     TFKDVAGLDEEKEDLQEVVDFLKNPKKYIELGARIPKGMLMVGPPGTGKTYLSRAVAG
                     EAGVPFFSISGSDFVEMFVGVGASRVRDLFEQAKKSAPAIIFIDEIDAVGRKRGAGLG
                     GGHDEREQTLNQLLVEMDGFGVNQGIIIMAATNRPDILDPALLRPGRFDRQVVVGTPD
                     VKGREAIFKVHSRNKPLSDDVKMDVLARRTPGFTPADIENLMNEAAILTARKREKKIK
                     METIEEAITKVIAGVAKKSKVISEKERRLTAYHEGGHAVCAHVLEEVSPVHQVTIVPR
                     GRAGGFTMQLPVEDKFYATKNEMKENIVVLLGGRVAEELVLKDVSTGASNDLERVTAT
                     ARSMVTKYGMSSKLGPMSFDSDDEVFLGNSFSSKRNYSEEVAFEIDQETKRIVDGAYD
                     KTRSILQENMDRLEYVAQALLIYETLDAEQFVKAFNKELPLNEIENAVTEENSSKEVE
                     EQLTIKLEKDEEERNNVIDINKNLEDKSDKDK"
     gene            complement(2154..3542)
                     /gene="tilS"
                     /locus_tag="ACOIAF_001730"
     CDS             complement(2154..3542)
                     /gene="tilS"
                     /locus_tag="ACOIAF_001730"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HGZ2668744.1"
                     /translation="MIFDKVLSTINKHNLIQKGDKIVLGLSGGPDSVCLLHVLNRLKK
                     DFNIEIYAAHLNHQIRGIEAQKDALYVSKLCEDMGIIFFVKSINVPKYCENEGLSLEE
                     GARKLRYEMFYEIKDKIKANKIAIGHNLNDQAETVMMRIMRGTGLKGLKGIDYIRDNC
                     IIRPILDVERNEIEEYCEAYNLNPRIDKTNLENIYTRNKIRLDLLPYMKDNFNSNVIE
                     SIVRMSNSLKSDNDYIEKEAEAKFREVSNIKEKGFVEINLDDFVCLHDAIKVRVLRNS
                     IKHILGDTNFVDQRHIEDIMSLEDNSKVNKMLTLPRNIFVYRKKDSIILTNEEIVNEE
                     IEFYYNVPSNGFIKIKELKQIIETQVMSIDRYKSMKLDNSSKGFDFNKVKGGIVIRSR
                     RQGDKIKLAMGSKKVKDLFIDLKIPREERCKIPIITDSEGIICVGDYKISENYKIDEN
                     TKEVLKINFNKL"
     gene            complement(3725..4153)
                     /locus_tag="ACOIAF_001731"
     CDS             complement(3725..4153)
                     /locus_tag="ACOIAF_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HGZ2668745.1"
                     /translation="MEEKSLSVKISINTRQYDEKGNMDTIEMTAFGKIFYKNDGIYVI
                     YKEKEEDIEITNTIKILKNQVSIKKFGAINSTMLFKCGESSTTKYVTPQGTLLIDIDT
                     RELDINIQEGEHIKLKIDYNIKVQDLFLGRNKIDIYIGTK"
     gene            complement(4170..4976)
                     /gene="murI"
                     /locus_tag="ACOIAF_001732"
     CDS             complement(4170..4976)
                     /gene="murI"
                     /locus_tag="ACOIAF_001732"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HGZ2668746.1"
                     /translation="MNNKPIGVFDSGLGGLTVLKEIMKILPNEDIIYFGDTARIPYGS
                     RSKETIIKYTFQAINFLKTKGVKAIVIACNTATARSLKEAQEKYDIPIIGVIEAGART
                     AVSSTKNKIVGIIGTEGTISSKAYNLEISKIDESIEIVNKACPLFVPIVEEGWANTEV
                     AKLTAKIYLQELKEKNIDSLVLGCTHYPILKRTIGEEVGEHIKLVNPAKETAKDLKKI
                     LEVQNIINNTEIHGTYQYYVSDIPEKFSDIAREFLKKKIDKIQKVEIQKY"
     gene            complement(5178..5663)
                     /locus_tag="ACOIAF_001733"
     CDS             complement(5178..5663)
                     /locus_tag="ACOIAF_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HGZ2668747.1"
                     /translation="MATLLALTFIFMPIKEVFAYEPTENIKKVNKQEKNIAKKEVINL
                     SDNDLMLLSKLVAGEARGESYEGQVAVAAVVINRVLDPRFPDTIEEVIYQKNAFSVVL
                     DGSIKMAPTDSARKAAQEALYGTDPTNKAVYFWNPEIATCKWINRLNPYMRIGNHVFA
                     K"
     gene            complement(5714..6373)
                     /gene="spoIIR"
                     /locus_tag="ACOIAF_001734"
     CDS             complement(5714..6373)
                     /gene="spoIIR"
                     /locus_tag="ACOIAF_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HGZ2668748.1"
                     /translation="MRKIKIRLGLLILSLISVISIMTIVINGEVKKVDNISKDYKDKL
                     IRFHVIANSNTDEDQELKLKVRDEVIKYLQPKLQNSKSIKESEAIIKKEYSSLEEISK
                     NIILKNGYNYSVKVGIQYSNFPTKQYSNIVLPAGEYKALKIIIGKGEGKNWWCVMFPP
                     LCFVDESNGVIDKSTDDKLKEVLTDKEYKLIKQDTPKKTSRVKIKFKVLEVVKDLEKK
                     F"
     gene            complement(6518..7198)
                     /locus_tag="ACOIAF_001735"
     CDS             complement(6518..7198)
                     /locus_tag="ACOIAF_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGZ2668749.1"
                     /translation="MENLTKLNLDNYKPLRDVVFENLREAILEGKLKPGQRLMEVQLA
                     EQLGVSRTPVREAIRKLELEGLVVMLPRKGAYVANMSLKDIMDVLEVRASLEGLAAYL
                     AAERISDEDIKKLKDISEEFKKSTLESDVDALLKLDVEFHECIFKATNNKKLIQLINS
                     LWEQVYRFRVTYMSAYDTSHNIVEEHKYLLDAIIKGDSELSKKYATEHIEKAEQFMID
                     KAMISKDL"
     gene            complement(7182..8072)
                     /gene="ispE"
                     /locus_tag="ACOIAF_001736"
     CDS             complement(7182..8072)
                     /gene="ispE"
                     /locus_tag="ACOIAF_001736"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HGZ2668750.1"
                     /translation="MDFIRLKSRAKINLSIDVLGKRQDGYHFVEMIMQTIDLYDIVKI
                     KELDEDEIKVKSTSLDIPLDEDNIVYKAAKILKNKFYIKKGVEIFIEKNIPVAAGMAG
                     GSSNAAAVLVGLNHLWELRLSEDELKEIGLNLGADVPFCISGRPALAQGIGEKLTNIK
                     GLPCDTNILICKPDLFVSTKEVYQGLDLNNIKKRPNNKYLIECLKSEDIKAVSESMVN
                     ILENVTIGKHKEISDIKQVMMKNNALGSMMSGSGPTVFGLFKNKEDALIGKKELLKKY
                     KQVYVVNSSQKGVEICGEFN"
     gene            complement(8190..9740)
                     /locus_tag="ACOIAF_001737"
     CDS             complement(8190..9740)
                     /locus_tag="ACOIAF_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOCS domain-containing protein"
                     /protein_id="HGZ2668751.1"
                     /translation="MELIKDVIKVDNRIDFGKFQTFIEAEAVVPDKKLDVYEIVKTEG
                     YISLKKIEMADGKILCRGSFNYNVIYIADDKNTISNVDGKVDINEVIEKDNVIQDMEY
                     MLFSEVEHMDCTIMNERKIKVGALMNIRGSLFEKQRLDIVKDVAQVEGIQKHRKEICF
                     QDIVGIEKAESSIRDTITINTEEIQSIISLNPCVKVKESRVTDNKVIIGGVLEINPLA
                     CTYEGELVELDRVGIEFTQFVEVPGVSDGMTEEVLLSMSDFNHIFKQNSESNTGLLEI
                     DCMACCKVKVTDEVTREVLQDAYSPQKIIKFDHKPIQLNKTLRRSEETFMVREGIRND
                     NDDIQIKDIVSVCPTMSIENSYIEGNKSIIQGIIKVEILFVPVEGLKLVYKISEEIPF
                     EHDIEMDNLTDTASVFNTACIDKVEVDLNRDQIDLIIKIKRFTEALDKKAENFIIKGE
                     DQGVYDLSKAPSIIVYICKEGDTFWNIAKKYNTTENEIAELNDIKLDEPIKPGKCLIL
                     EKKVVLVD"
     gene            complement(9834..10100)
                     /locus_tag="ACOIAF_001738"
     CDS             complement(9834..10100)
                     /locus_tag="ACOIAF_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HGZ2668752.1"
                     /translation="MATVQTLDKIRVSLERHIGKKILLKANKGRKQIITKEGILEKVY
                     PSVFVIKLDDESNGYPRVSYSYSDLLTSNVKLQVFRDQDKLQIS"
     gene            complement(10248..11108)
                     /gene="yabG"
                     /locus_tag="ACOIAF_001739"
     CDS             complement(10248..11108)
                     /gene="yabG"
                     /locus_tag="ACOIAF_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation peptidase YabG"
                     /protein_id="HGZ2668753.1"
                     /translation="MKVGDIVARKSHNKDIVFKIVSFGVDENNEKIAILKGIAFRVIA
                     DAYIDDLELVKAPDIKDILIDKNVENLLYKSVRKAKERQKRMTRAVPKLQLNPNTYGM
                     PGKVLQIDGDKEYLKICLDVYAQLGIPAVGVAVSEQNQYKEVRALLEKYNPDILVITG
                     HDAMTIKRGDIEDMSNYRNSSNFVKTVKEARKWQPNLDNLVIFAGACQSNYEKIISAG
                     ANYASSPGRIMIHALDPVFIVEKIACSRIDVVVPIDEVIEQTITGVKGIGGSETRGKF
                     RWAMPKTILH"
     gene            complement(11192..12130)
                     /locus_tag="ACOIAF_001740"
     CDS             complement(11192..12130)
                     /locus_tag="ACOIAF_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HGZ2668754.1"
                     /translation="MKFIDLHCDTIAKLMENVETSELKSNKYSVDIDRLKKGDSLAQT
                     FALFVDTEEVKHPFDYCMSMANKFHEEMKKNSDEIALATNYEEIMKNQSEGKLTALLS
                     IEEGAVLEGKLENLKKFYDLGVRMMTISWNHVNELSFPHNKVEYREKGLTDFGREVVH
                     KMNELGMLVDVSHISDGGFYEIAKISSKPIIATHSNSRAMMNHSRNLTDDMIKVLANK
                     GGVTGINFFHLFLSDKSESKLEDMVRHIKHIVNVGGIDVVSLGSDFDGIDSKVEIEDI
                     SQMGKLYEPLKKEGFSEDDIEKIYYKNALRVIKEVL"
     gene            complement(12240..12401)
                     /locus_tag="ACOIAF_001741"
     CDS             complement(12240..12401)
                     /locus_tag="ACOIAF_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668755.1"
                     /translation="MFILRFADDDDNLSVKDFTSLTDLKKYISENNIDKTWHQIEEVK
                     KVIPNLKEE"
     gene            complement(12411..12908)
                     /locus_tag="ACOIAF_001742"
     CDS             complement(12411..12908)
                     /locus_tag="ACOIAF_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HGZ2668756.1"
                     /translation="MSLYTEWRNLSDNHESQEAEVKFWEEYLKVEAGIYNEILNNKMD
                     VVEGKVSELAEKFGTTNEFFMGFIDGISESLKEDIVLEEVEADKEVSIKIDWEKLYNN
                     MVTVEAHWLYNLQGWEDILPADQRKEIIKAYKKSKTIVKEAKIGRNDSCPCGSGKKYK
                     KCCGK"
     gene            complement(13023..13733)
                     /locus_tag="ACOIAF_001743"
     CDS             complement(13023..13733)
                     /locus_tag="ACOIAF_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0489 family protein"
                     /protein_id="HGZ2668757.1"
                     /translation="MENYEYSGFYIEKPVGNNVFSYDKRENKSIYVPKLINGTLNDVR
                     LGNEVVFNEVDENKEIKAKGLENMVEYVLGNKKIYVFDNHNHAFYFWAKSLLKGEFTK
                     GCKLVHVDQHKDTREPENYDVDVNNLKDVFRYTNEVLNVGSFIKPALKYNIFSELIII
                     DSLYGFDLEVESEFVLDIDLDIFSSDMDYIPFELKFYKIKNLIKKAKVITIATSPYFI
                     NQEYAIKVLKELFNYDII"
     gene            complement(13909..14439)
                     /locus_tag="ACOIAF_001744"
     CDS             complement(13909..14439)
                     /locus_tag="ACOIAF_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668758.1"
                     /translation="MDIRALPIRITKEEAIEIAKSGKNSLFSKLILKNKYAHEVRLHY
                     IEFKIITLKIHKTKSIFKNRRYSNDINELKILVNGSTGSGSIVQDFPTYEVVKNVDCN
                     IVQYSDKDERRVKAKANKMAMRLTHRFMGGIPEIEIVKIESIFRPYWVVFFGKVELNR
                     RVSYLPIEADGFVIGM"
     gene            complement(14456..14686)
                     /locus_tag="ACOIAF_001745"
     CDS             complement(14456..14686)
                     /locus_tag="ACOIAF_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668759.1"
                     /translation="MSKLVSKVKKDLEIKWLGIDGFLVLLYFLITGFIFYGILTRNDI
                     IVGKIIPYGVMAVVIVIFFDYIYRVLKMTRKK"
     gene            complement(14701..16095)
                     /locus_tag="ACOIAF_001746"
     CDS             complement(14701..16095)
                     /locus_tag="ACOIAF_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HGZ2668760.1"
                     /translation="MLTFWENGIIVIFAFILLGIGYYFSRNVKDMESYYLGNRSLPWS
                     LIVGTLVASWYGGVGTVGSVEYAAIYGISAWAVWSIAAHIGRMPLALWVGPRIHIRTN
                     ITVPDLLESFYGKHVAIIGAILMFIYCAQLGEITAMGTIGNVAWKIDKELIGLIMVGV
                     VVVLTVLGGLMGVAITDMVLFFCMVFGLTMVMPGQFEQIGGFQGLTEALKDAPQLMHP
                     TKGMSAMKALMLIVYSFGAYSDPTFYQRFSAADSPKVGRRALLTCFSLWICFDMVLTM
                     TGLIVRATYPEMAPAAGYITLVLDTLPQGIRVIFIIGILGSIISTLDSYYLAAGATLA
                     NDIYGRITKKELSQKQLVKFTRIGVCLAAVMGLSVAFRFENVYDACIFVGSIWMGSAF
                     VPIVGGLLGNGRRTSMGGILGMIVGGVTFGYFKIFPVQSFELKPLVIAIPFSFIAWMI
                     GNRIGENLNTQNNL"
     gene            complement(16221..17474)
                     /locus_tag="ACOIAF_001747"
     CDS             complement(16221..17474)
                     /locus_tag="ACOIAF_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HGZ2668761.1"
                     /translation="MESKNNVKRFLIIFILAFGTTAMYSLPYMKSSFYDPMQQALALS
                     HTQIGNLLSLYGLVGMVSYFIGGWFADRFSVRKLITFSLIASGILGFYFSTFPSYSMI
                     LLIFVLWGFTTILTFFSASVKVVRMQGSESEQGRIFGFYEGLSGVSGTLISFIGLYFF
                     GKFAEITVGFKYVVWLYSAASIICGILLFFLVEEKKDSGASDEGLSIKSLLKVVTMPK
                     AWLIGLIIFSTYLVFSSLTYLSPYLSEVYVMPMTLVSALSIIRTYVIKMGASPVAGVI
                     TDKVGSSIRVMFVGFILMTVSTAAYLVIPKSTGFIWIAVINMIILSVILFGFRGIYFA
                     SVSESNISLETTGAVVGFASFIGFSPDAFYYTIAGNWLDKYGQTGYTYIFILSVVCAV
                     IGIFATYALNKINKRENKGLNNKIA"
     gene            complement(17525..18679)
                     /locus_tag="ACOIAF_001748"
     CDS             complement(17525..18679)
                     /locus_tag="ACOIAF_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HGZ2668762.1"
                     /translation="MSNNINQNLETKIIHWGHSADPTTGALATPICQTATFAAKTVEH
                     FEELCMTWGYVYTRECNPTLTELEAKLAMLENAESAISSTSGMGAITSTILALVKSGD
                     HIVSSDGIFSHTKLFMSELLSKFGVEVTFVDAVNPQNVKEAMRPNTKIVYIESPLNPS
                     LDLVDIKTIAEIAHENKSLAIVDSTFGTPIVQRPIDLGADLVIHSLTKFINGHGDTLG
                     GAVAGSKELIDLVRWPSLCCFTGASLPPMSAWMILRGMKTLDMRMKKHCENGLAVAEF
                     LEEEENVELVKYPALKSHPQYELCKTQMNGLGGGVVSFKLKDGINGLTRDQASRRLMN
                     SLELATIATSLGEEHTLVQMNGENLIRIAVGLESSNDIINDFKQAIEKLK"
     gene            complement(19239..19808)
                     /locus_tag="ACOIAF_001749"
     CDS             complement(19239..19808)
                     /locus_tag="ACOIAF_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HGZ2668763.1"
                     /translation="MIIIYGYCRVSTKNQTDNNSLYQQQQEILNKYNDAKIIKETYTG
                     TTTNRPKFEKIISSLKKGDLLVATKLDRLARSTSEGIQLIEGLFAKEVSVHILNIGLL
                     ENTSMGKFFLTTLLAVAELERNMIIERTQLGKEFAKKKKDFKDGRPKKYSNQQIQYAL
                     GLLNSKPYREVVKMTGISKSTLIRAKKSI"
     gene            20011..20238
                     /locus_tag="ACOIAF_001750"
     CDS             20011..20238
                     /locus_tag="ACOIAF_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HGZ2668764.1"
                     /translation="MNSLLKEAIDELKNIHCGEVFLLKELFKGYEWNRLEMNIRLKLG
                     KLFLYEVVTNPNLNVEVINKNSAYQQMYKKK"
     gene            complement(20457..21200)
                     /locus_tag="ACOIAF_001751"
     CDS             complement(20457..21200)
                     /locus_tag="ACOIAF_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HGZ2668765.1"
                     /translation="MENYQSTNTCTYNILYVQFNEDFERSKYFSYDVKYPVIYNIRNQ
                     STYVNPVILNNINNAVRMTVNNFKNGLQEEEREYNNAAPQNSLPKQSYWVSTSYAVTF
                     NKNYVLSMILNLMGFANNYGPKYNTLNNYNIDLTTGKELLLNDIFRDGVDYINIITDY
                     IKSQINENREMYYSNVELVIPEDQAFYLTDNGIIIYFGVDEIAPESFGIPKFELKFSD
                     FGNFINLRFYCISPDIYVQSRGRAKHFMG"
     gene            21368..22240
                     /locus_tag="ACOIAF_001752"
     CDS             21368..22240
                     /locus_tag="ACOIAF_001752"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGZ2668766.1"
                     /translation="MLLLADKWKDYELIDMGNGEKLERWGSYVLRRPDPQVVWPMESE
                     WALWKNPHGHYHRSNKGGGQWEHKKRYPEQWTINYKNLKFHIKPTGFKHTGLFPEQAA
                     NWDWMIEKIKKANRPIKVLNLFAYTGGATVACASAGAEVCHVDAAKGMVNWAKENIEL
                     SGLKNQTVRFIVDDVVKFVEREIRRGKKYDAIIMDPPSYGRGPKGEVWQIEEKLYSFV
                     DLCMGVLSDNPLFFLINSYTTGFSPIVLENVLSTTIGKKTNTGDIYGGELGIPASRDG
                     KILPCGIFGRWEKK"
     gene            22240..22905
                     /locus_tag="ACOIAF_001753"
     CDS             22240..22905
                     /locus_tag="ACOIAF_001753"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HGZ2668767.1"
                     /translation="MIKVIYEDNHLLVVEKPVNILSQGDNTNDKDMVNLLKDYIKKKY
                     NKPGNVYIGLIHRLDRPVGGVMVFAKTSKAASRLSEQVRSKTFKKTYLAVVHGKIKSN
                     SDILKDFLYKNKKTNMVTVVKKSHKDAKEAELNYNVLDFKENLSLVQVNLKTGRSHQI
                     RVQFASRKHPLYGDQRYGKEINKIGQQISLWSNKIEICHPTTKEKMEFTCHPPDKYPW
                     NIF"
     gene            22926..23672
                     /locus_tag="ACOIAF_001754"
     CDS             22926..23672
                     /locus_tag="ACOIAF_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HGZ2668768.1"
                     /translation="MSKKFSIGEMSKLHKISIQTLRYYDQIDLFKPVHVDQESNYRYY
                     SIDQFSQLDIIKYLKYLGMPLKEIKSKLDENNEEILKLLTNKINIVDEKIKELELIKK
                     VLSTKKETMELSRRQIANILEDNISVFYGGVSGLVSIDKFLKEDKVIYDNSFVTVERD
                     LFDIKSQKHITEISSGDFICMTYKGPYKENYTAFKKLVDYVEYKRIPIENYIYEIPII
                     DPLSTNNEEELLTEIQIQVKKREIDLRCLV"
     gene            complement(23828..25552)
                     /locus_tag="ACOIAF_001755"
     CDS             complement(23828..25552)
                     /locus_tag="ACOIAF_001755"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2668769.1"
                     /translation="MLAIIMLGSIFLIYNSFNMSLNERTHQFGILSSVGATAKQLRNS
                     VLFEGICIGAIGIPIGVAIGIVSIGLVISVIAKNFANILYVNVPLTLTLSIPAIIVAA
                     VVSMVTILISAYIPARKAANTPVMECIRQTNEVKIESKSVKTSKLTQRVYGLEGVLAM
                     KNFKRNKKRYRSIVLSLVLSIVLFISTSALVADLKQMSNQRSEVTDQDIIFSTQGMKD
                     NEMLKLYDKLKTANGVYEGLYQKLMTYSCVAKVSELTDDYLNYEGSHSMSEMLELPVD
                     MQFLDDRTYLNIIKSLGLPAEEYTGQNAKLIAVSMVTKDNNIIDIFNSSSMNVSLTPE
                     TNGKLEIPERQNVNISFVKVVPPDSPPGIRSSDVKKASFRVLAPYSAKDKFEIPGVST
                     GIESLSFQSKNPTQSVAEMEQIIESEGVTNEYNLYNMYGMMDENRNYIFIANVFAYTF
                     IIMISLISIANVFNTISTNIKLRRRELAMLRSVGMSEHDFQKMMNFECAFYGMRALFF
                     GLPIAVISSWLIHKVMVTDDIDFVIPWFSIGISIFSVLFIVFITMLYTINKIKKENII
                     DALRDDMA"
     gene            25542..25667
                     /locus_tag="ACOIAF_001756"
     CDS             25542..25667
                     /locus_tag="ACOIAF_001756"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668770.1"
                     /translation="MASTMPPTEYKNILNKLLSETPMKRNTLSLKMEAWSLVLLV"
     gene            complement(25826..26374)
                     /locus_tag="ACOIAF_001757"
                     /pseudo
     CDS             complement(25826..26374)
                     /locus_tag="ACOIAF_001757"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(26374..27060)
                     /locus_tag="ACOIAF_001758"
     CDS             complement(26374..27060)
                     /locus_tag="ACOIAF_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2668771.1"
                     /translation="MAFLKIEDLCKVYGKNENKVTALDHVSLTIEKGEFTAIIGSSGS
                     GKSTLLHSIAGVDVPTSGKIYLEGQDVYGQSNEKLAIFRRRQVGLIYQFHNLIPTLNV
                     VENITLPILMDKRKVNQERLNDLLELLGLKERKTHLPNQLSGGQQQRVAIGRALMNAP
                     AVMLADEPTGSLDSKNGQEIIQLLKESHSKYHQTLIIVTHDENIALQADRIICISDGK
                     VVRDERKVNR"
     gene            complement(27143..28132)
                     /locus_tag="ACOIAF_001759"
     CDS             complement(27143..28132)
                     /locus_tag="ACOIAF_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HGZ2668772.1"
                     /translation="MFRNREFQKFAILFLLITILTVALGFAISIMTGILSIVSATTFG
                     IAFFVFTKNRYKSIAQISEQINLVLHNANHLYIAESDEGELSILQSEITKMTLRIREQ
                     NYALKKEKEHLADSLADIAHQLRTPLTSVTLILSLLENTSDEDERKELIRETEELLIR
                     MDWLITSLLKLSRLDAGIVVFQKEQIDVNNLISSALHQLLIPMELHNITLHIDIPKGV
                     RILGDLNWLSEAIQNIFKNCMESVGDNGKIDIICEDNFLFTQLTIHDNGAGFKKEDLP
                     CLFNRFYRGKNSSTAGYGIGLALCKTIIMRQGGTITAQNHPQGGAIFVIRFPK"
     gene            complement(28119..28805)
                     /locus_tag="ACOIAF_001760"
     CDS             complement(28119..28805)
                     /locus_tag="ACOIAF_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGZ2668773.1"
                     /translation="MKRIFLVEDDKAIAKNLILLLNSEGFIVTHVSTRSEALVEIAKN
                     KFDLALIDISLPDGNGFTICTEIKEKQDIPVIFLTASGDESSVVTGLNMGADDYITKP
                     FRPRELIARIRTALRKSGRFGSTFEICGLYVDTATGVVKKNGNEVFLSALEYRLLLVF
                     ISNPKSIITRVRLLDELWDAAGEFVNDNTLTVYIKRLREKIEDDPSSPQIILTVRGTG
                     YRLGGSYVSK"
     gene            complement(28802..28924)
                     /locus_tag="ACOIAF_001761"
     CDS             complement(28802..28924)
                     /locus_tag="ACOIAF_001761"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668774.1"
                     /translation="MDYIRTKETTENCVMISKIVVYYCSAGIYKTNKIKEDESK"
     gene            complement(29305..>29586)
                     /locus_tag="ACOIAF_001762"
     CDS             complement(29305..>29586)
                     /locus_tag="ACOIAF_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006441050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668775.1"
                     /translation="KSLRENGIEADIVNRIEEPRPNILDAIRNKQVDIVINTPTKGND
                     STRDGFKMRRIAIEFSTEIMTSLDTLKALVEVKKKHLNKDKLKVYNIAE"
BASE COUNT         9612 a         5370 c         3386 g        11218 t
ORIGIN      
        1 taaataattt taaaataata aaaaaactga aagtatcaat taaattaata catgcaaata
       61 taatttgctt attaatttaa tattatactt tcagttttag tatttaatcc acatcagata
      121 ataatcctaa aatgaccaag gtcttaaaac tatttatctt tatcagattt atcttctaaa
      181 tttttattta tatcaataac attgtttctt tcttcttcat ctttttctaa ttttatagtt
      241 aattgttctt caacttcttt agaagaattt tcctctgtaa ctgcattttc aatttcattc
      301 agaggtaact ctttattaaa tgcttttaca aattgttctg catctaaagt ttcatatata
      361 agtaaagctt gtgctacata ctctaatcta tccatatttt cttgtaatat acttcttgtc
      421 ttatcatatg caccatcaac tatacgcttt gtttcttggt ctatctcaaa agcaacttct
      481 tcagaataat ttctcttact tgaaaaacta ttacctagga atacttcatc atcactgtca
      541 aatgacattg gaccaagttt agaactcatt ccatactttg ttaccatact tcttgcagta
      601 gctgtaactc tttctaagtc atttgaagca cctgtagata catccttaag tactagctct
      661 tctgctactc taccaccaag taaaactaca atattttctt tcatttcatt ttttgtagca
      721 tagaacttat cttcaacagg aagttgcatt gtaaaccctc cagctcttcc tcttggaact
      781 attgtaactt gatgaacagg acttacttct tcaagtacat gggcacaaac tgcatgccct
      841 ccttcatggt atgctgtaag tcttctttct ttctcactta ttactttcga ttttttagcc
      901 acacctgcta taacctttgt tatagcttct tctatagttt ccatcttaat tttcttttct
      961 ctttttctag ctgttaatat tgcagcctca ttcattaagt tctctatatc agcaggagta
     1021 aatcctggtg ttcttctagc caagacatcc atttttacat catcacttaa tggtttattt
     1081 cttgagtgaa ctttaaatat agcctctcta ccttttacat ctggtgtacc aacaacaact
     1141 tgtctgtcaa atcttcctgg tctaagtaaa gctgggtcaa gtatatctgg tctatttgta
     1201 gctgccatga ttattatacc ttgatttacc ccaaatccat ccatttcaac tagaagttga
     1261 ttaagagttt gctctctttc atcatgacct ccaccaagac ctgcacctct ttttctacca
     1321 actgcatcaa tttcatctat gaatatgata gctggagcac ttttcttagc ctgttcaaat
     1381 aaatctctta ctcttgaagc accaacccca acaaacatct caacgaaatc agaaccactt
     1441 atactgaaaa agggaactcc tgcttctcct gcaactgctc ttgatagata tgttttacca
     1501 gttccaggag gtcctaccat aagcattcct tttggtattc ttgcacccaa ttcaatatat
     1561 ttcttaggat tctttaagaa atctactacc tcctgcaaat cttctttttc ctcatctagt
     1621 ccagctacat ctttaaatgt aactctagtt ttttcatcat ctttatgaac cttagctttt
     1681 gatttgccaa aactcataac ctttccgcct ccaccttgag actgattcat aaacacgaac
     1741 caaataatca ccatgaagaa tattaacagt aatgtaggca acatttcgac aaaccaaggt
     1801 gtagatggct tagcttcacc accaaaagtt agtttcccag ccttagcttg gtctaacacc
     1861 tcatctgcaa gtttatttcc catcacttct gttggaacat atgacttaaa ctttgtattt
     1921 gtatctttta tagtaccttc tacagaagtt tgatttacaa agtataatct tgatatattc
     1981 tcatctgtta attctctata tacttcagag aattttaaat cttttatttt ttctgtaggc
     2041 ttacctgaga attgaactat acctactata attataaaaa caagtagata aaaacctgcc
     2101 ccttttagca acttattcaa tatagaagcc ctcctttcat acattgcttt gccctataat
     2161 ttgttaaaat taatttttaa tacttctttt gtgttttcat caattttata attttcactg
     2221 attttataat ctccaacaca tattattcct tcactatctg tgattatagg aattttacat
     2281 ctttcttctc ttgggatttt taaatctatg aataaatctt tcactttttt acttcccata
     2341 gcaagcttta tcttatcacc ttgccttctg cttcttatta ctataccccc ttttacctta
     2401 ttaaaatcaa accctttaga tgaattgtct aatttcatac tcttatacct atctatgctc
     2461 attacttggg tctcaataat ttgttttaat tcttttattt taataaaccc attactaggt
     2521 acattgtaat aaaattcaat ttcctcatta acaatttcct cattcgttaa tattatactg
     2581 tctttttttc tataaacaaa tatatttctt ggaagagtta acattttatt tacttttgaa
     2641 ttatcttcta aagacattat atcttctata tgtctttggt caacaaaatt agtatctcct
     2701 agtatatgtt ttatggaatt tctaagaact ctaactttga tagcatcatg taagcaaaca
     2761 aaatcgtcta aatttatctc tacaaagcct ttttctttta tatttgaaac ttctctaaat
     2821 ttagcttctg cctctttctc aatataatcg ttatcacttt ttaaactatt gctcatcctc
     2881 actatagatt ctattacatt tgaattaaaa ttatccttca tatatggtaa aaggtctaac
     2941 cttatcttat ttctagtgta tatattttct aaatttgttt tatctatcct cggatttaaa
     3001 ttataagctt cacaatactc ctctatctca tttctttcca cgtctaatat gggtctaatt
     3061 atgcaattat ctctaatata atcaattccc tttaagcctt ttaaacctgt tcctctcatg
     3121 atacgcatca taaccgtttc ggcttggtca ttgagattat gacctattgc tattttattg
     3181 gctttaatct tatccttaat ttcataaaac atttcatatc ttagcttcct tgctccttct
     3241 tctaatgata atccctcatt ttcacaatat tttggtacat taatagactt tacaaagaat
     3301 ataattccca tatcctcaca aagtttagat acatataatg catctttttg agcttctatc
     3361 cctcttattt gatggtttaa atgtgctgca tatatttcta tattaaaatc tttttttaat
     3421 ctatttagta catgcaataa acaaactgaa tcaggtcctc cagaaagacc taaaactatt
     3481 ttatcgcctt tttgtattaa attatgttta tttatagtac ttagtacctt atcaaatatc
     3541 atatttatcc ctcttacact tagttttatt cacttttaag tatactttct ataaaaaaac
     3601 gctaaaccct tgtatcaatt ttaagaaatt aaagtttaat ataaatataa taataccaaa
     3661 taaattaaat aaaaggtagt tttttcacta cctttataaa atttctggaa aatagccata
     3721 gttattattt agtacctatg tatatatcta ttttattcct acctaaaaat aaatcttgta
     3781 cctttatatt atagtctatt ttcaatttta tgtgttcccc ttcttgtata tttatatcta
     3841 attctcttgt atcaatatca attaataaag taccttgtgg ggtcacatac tttgtagtac
     3901 tactttctcc acatttaaac agcattgttg agttaatagc accaaacttt ttgatactaa
     3961 cttgattttt aagtatcttt atagtattag ttatttctat atcttcttct ttttccttgt
     4021 aaataacata tattccatca tttttataaa aaattttacc aaaggcagtc atctctatgg
     4081 tgtccatatt tcctttttca tcatattgtc tagtatttat acttattttt acactcaacg
     4141 atttttcctc catagattct tcatataaac tagtattttt gtatttctac tttttgaatc
     4201 ttatcaattt ttttctttaa gaactctctt gctatatctg aaaatttttc aggtatatct
     4261 gatacataat attgataagt tccatgaatt tctgtattgt ttataatatt ttgcacctct
     4321 aaaatctttt ttaaatcctt tgcagtctct ttagctggat taacaagttt tatatgctct
     4381 ccaacttctt cacctattgt tctctttaaa ataggataat gagtacatcc taacactaaa
     4441 gaatctatat ttttttcttt caattcttgt aaatatatct ttgctgtcag tttagccact
     4501 tctgtatttg cccatccttc ttcaacaata ggcacaaata aaggacatgc tttattaaca
     4561 atttctatac tctcatctat tttagatatt tccaagttgt atgcttttga acttatagtt
     4621 ccttcagttc ctattattcc tacaattttg ttttttgtag aacttacagc tgttctagct
     4681 cctgcttcta taactccaat tatcggtata tcatattttt cttgagcttc ttttaagctt
     4741 cttgcagtag cagtgttaca tgctataact atagctttta caccttttgt ttttaaaaag
     4801 tttatagctt gaaaagtata tttaattata gtttctttgg atcttgaacc atatggtatt
     4861 ctggcagtat ctccaaagta tattatatcc tcatttggta atatcttcat tatctctttt
     4921 agaactgtta atccacctaa tcccgaatca aaaactccta ttggtttatt gttcatcttt
     4981 atacaacacc tactttacat ttttgtgttt aacaacaact tatttatcgc taaaatttat
     5041 atttatattc ataattaata tattattacc taaatgatta ttatagttta ttttacacaa
     5101 aaaataaagg agctatctta aattagaaaa taatttctac aaaaagacaa ctcctttcac
     5161 tacttaaaaa tttaaattta ttttgcaaac acatgatttc ctattctcat ataaggattc
     5221 aatctattta tccatttgca agtggctatt tctggattcc aaaaatatac tgctttatta
     5281 gttgggtcag ttccataaag agcttcctga gctgctttac gtgctgagtc tgttggtgcc
     5341 atctttattg aaccatcaag cacaactgag aaagcatttt tttgatatat gacttcttct
     5401 attgtgtctg gaaatctagg gtccaatact ctatttatta ctacagctgc tacagcaacc
     5461 tgtccctcat agctttctcc tctagcttct cctgcaacca acttagataa taacatcaag
     5521 tcattatcag ataaatttat tacttctttc tttgctatat ttttttcttg tttgtttact
     5581 ttcttaatat tttctgttgg ttcatatgca aatacctctt taataggcat aaatataaaa
     5641 gtcaaagcta atagtgtagc catcagttta ctccacctgt ccatgattaa aaccactcct
     5701 tacattctta tttttaaaat tttttctcta aatccttgac tacttcaagt actttaaatt
     5761 taatttttac tctacttgtt ttttttggtg tatcttgttt tattaactta tattctttat
     5821 cagttaaaac ttccttcaac ttatcatctg tagatttatc tatcactcca ttagattcat
     5881 caacaaaaca tagtggaggg aacataacac accaccagtt ttttccttca cctttcccaa
     5941 tgataatctt taatgcttta tattcaccag ctggaagaac tatattagaa tattgtttag
     6001 tagggaaatt gctatattgt atacctacct ttacactgta attatatcca tttttaagaa
     6061 taatgttttt acttatttct tccagactac tatattcctt ttttataata gcttcacttt
     6121 cttttatact ttttgaattt tgaagctttg gctgtaaata ttttattact tcatctctaa
     6181 cttttagttt aagttcttgg tcttcatctg tattgctgtt tgctataaca tgaaatctta
     6241 ttaatttatc tttataatct tttgatatat tgtctacttt ttttacctca ccatttatta
     6301 ctattgtcat tattgatatg actgatataa gacttaatat taatagtccc aatctaatct
     6361 taatttttct cattgaaaat tccccctcaa gataaattta tatcatcaat tatgtccaat
     6421 taaatttaat ttattcataa tattttttaa tactaataaa aaaagctatc ttaaaatgat
     6481 tacaatcatt ttaaaatagc tttttaaacc ttatatttta taagtcttta ctaatcatag
     6541 ccttatctat cataaactgc tctgcttttt ctatatgctc tgtagcatac ttcttggata
     6601 gctcactatc acctttaatt atagcatcaa gtaaatattt atgctcttca acaatattat
     6661 gtgatgtatc ataagcagac atataggtaa ctctaaatct atatacttgt tcccacaatg
     6721 agtttattaa ttggattaat tttttattat tagttgcttt aaatatacat tcatgaaatt
     6781 ctacatcaag ttttaataac gcatctacat cggattccaa agtgcttttt ttaaattctt
     6841 ctgatatatc tttaagcttt ttaatatctt catcactaat tctttcagcg gcaagatatg
     6901 ctgctaagcc ttctaggctt gctcttactt ctaatacatc cataatatcc tttaatgaca
     6961 tatttgccac atacgcacct tttctaggca acataacgac taaaccttct aattcaagct
     7021 ttcttattgc ctctctcact ggagtcctac ttactcctaa ttgttctgct agttgcactt
     7081 ccattagcct ttgaccaggc tttaattttc cttctaagat tgcttctctt aaattctcaa
     7141 agactacatc tcttaatggc ttgtaattat ccaagttcag tttagttaaa ttctccacaa
     7201 atttcaaccc ctttttgact gctattaaca acatacacct gtttatattt ttttaataat
     7261 tctttttttc caattagagc atcttcttta tttttaaata gtccaaaaac tgtaggacca
     7321 cttccactca tcatagaacc taaagcatta tttttcatca ttacttgttt tatatcactt
     7381 atttctttgt gtttacctat agtaacattt tcaagaatat ttaccatgct ttctgataca
     7441 gcttttatat cttcagattt taaacactct atcaaatatt tattatttgg tcttttttta
     7501 atattattca aatctaatcc ttgataaact tctttagtag aaacgaataa gtctggtttg
     7561 catatcaata tatttgtatc acatggtaaa cctttgatgt ttgttagctt ttctccaata
     7621 ccttgagcta aggcaggtct tcctgatata caaaaaggaa catctgcgcc caaattaagt
     7681 cctatttctt ttaattcatc ttcagataat ctaagttccc ataggtgatt taaacctact
     7741 aatactgcgg ctgcattaga acttcctcca gccatccctg ctgctacagg aatatttttt
     7801 tctatgaata tttctacacc tttttttata taaaatttat ttttaagtat tttggcagct
     7861 ttataaacaa tattatcttc atctaaagga atatctaaac tagtgctttt tacctttatt
     7921 tcatcttcgt ctaattcttt tatctttact atatcgtata aatctatcgt ttgcattatc
     7981 atttcaacaa aatgataacc gtcttgcctt tttcccaaga catcaatgga taaatttatt
     8041 tttgctctac tctttagtct tataaaatcc attctatcct ccttgtttaa agaataatgc
     8101 atcactaaca ataatattat actacaaaaa ataaaataat agggtattat aataccctat
     8161 tatacttaat tactttaaat tttttaatat taatctacta atacgacttt tttttctaaa
     8221 ataagacact taccaggctt aattggttca tcaagtttta tatcattaag ttctgcaatt
     8281 tcattttctg ttgtattgta tttttttgct atattccaga aagtatctcc ttctttgcat
     8341 atatatacga ttatgctagg agctttagat aaatcatata caccttggtc ttctcccttg
     8401 ataataaaat tttctgcttt cttatctaat gcttcagtaa atctcttaat tttgataatc
     8461 aagtctatct ggtccctatt caaatcaact tcgaccttat caatacatgc tgtattaaac
     8521 acagatgctg tatcagttag attatccatt tctatatcat gttcaaatgg aatttcctca
     8581 ctaattttat aaaccaattt taaaccttca actgggacaa ataaaatttc aacttttatg
     8641 attccttgta taatgctttt atttccttca atataactat tttctataga catggttgga
     8701 caaacgctta caatatcttt tatttgtata tcatcattat catttcttat gccttctcta
     8761 accataaatg tttcttcact acgtcttaaa gtcttattta attgaatagg tttgtggtca
     8821 aattttatta tcttttgagg cgaatatgca tcttgtaata cttctcttgt aacttcatct
     8881 gtaactttta ccttacaaca agccatacaa tctatttcta aaagacctgt gttgctttca
     8941 ctattttgct tgaaaatatg attaaaatct gacatagaca acaatacttc ttcagtcata
     9001 ccatcactaa ctccagggac ttctacaaat tgagtaaact caatacctac tctatccaac
     9061 tctacaagtt caccctcata tgtacaagct aacggattta tttctaatac tccaccaata
     9121 ataactttat tgtcagtcac tctactttct tttactttaa cacatggatt taaacttatt
     9181 atagattgaa tttcctctgt gtttattgtt atagtatctc ttatagaact ttctgctttt
     9241 tctattccaa ctatatcttg aaaacaaatt tcctttctgt gcttttgtat gccttccact
     9301 tgcgccacat cttttacaat gtctaacctt tgcttttcaa acaaacttcc ccttatattc
     9361 ataagagctc ctactttaat ctttctctca ttcattatag tacaatccat atgctctact
     9421 tctgaaaata acatgtattc catatcttga ataacattgt ctttttctat tacttcgttt
     9481 atatctactt ttccgtctac attagaaata gtatttttat catctgcaat atagattaca
     9541 ttataattaa aactacctct gcaaagtatc ttcccatctg ccatttcaat ttttttaagt
     9601 gatatgtatc cttctgtttt tacaatttca tatacatcta attttttgtc tggtacaact
     9661 gcctccgctt caatgaatgt ttgaaatttt ccaaagtcta ttctattgtc aactttaatt
     9721 acatctttaa ttaattccat agtctacctc cttaagtatt ataaaaatat cacactatat
     9781 ttatatttaa ggagcaaaaa aaaaattaca cctattttta aataggtata attttaactt
     9841 atttgtaatt tatcttgatc tctaaaaact tgcaacttta cattagatgt taataaatct
     9901 gagtaactat aagatactct aggatatcca ttactttcat catctaactt tataacaaac
     9961 acgcttggat aaactttttc tagtataccc tcttttgtaa taatctgctt tcttccttta
    10021 ttagctttca acaatatttt ctttcctata tgtctctcta agcttactct tatcttatct
    10081 agagtttgaa cagtagccac aatatcacct tctttaacgt ttatatgttt acataatatc
    10141 acatataaac atttttgtca aatgtttaaa ctaattattt tatatatttt ttgcaaaaat
    10201 gtcaatacta tttttgaaga tatacagtat aattatgtta atttttgcta atgtaatatt
    10261 gtttttggca ttgcccatct aaattttcct cttgtttcag aaccaccaat tcccttaaca
    10321 cctgtaatag tctgctctat aacctcatca ataggaacta ctacatcaat tcttgaacaa
    10381 gcaattttct ccactataaa aacagggtct aatgcatgta tcattattct tccaggcgaa
    10441 cttgcatagt ttgctcctgc acttattatt ttttcataat ttgattggca tgctcctgcg
    10501 aatatgacaa gattatccaa atttggttgc catttacgtg cttcttttac tgttttaaca
    10561 aaatttgaag agtttctata gttactcata tcttctatat ctcctctttt tattgtcatt
    10621 gcatcatgac ctgttattac tagtatatcc ggattatatt tttctagtag tgctcttact
    10681 tctttatact gattttgctc tgaaacagct acacctactg caggtattcc aagttgagca
    10741 tatacatcca agcaaatttt taaatattct ttatctccat ctatctgtag tacttttcca
    10801 ggcattccat atgtgttagg atttaattga agttttggaa ctgctctagt cattcttttt
    10861 tgtctttctt tagcttttct tactgactta tataataaat tttctacatt tttatctatt
    10921 aatatatctt ttatatcagg tgcttttact aattctaggt catcaatata agcatctgct
    10981 attactctaa atgcaattcc ctttagtata gctattttct cattattttc atctacacca
    11041 aaagagacta ttttaaatac tatatctttg ttatgtgact ttctagctac aatgtctcct
    11101 accttcatag ttaaacctcc aataaatttt tatcttagtt tatactatgt tcgatataaa
    11161 actttgtttc ctaatgtcaa aaaaacattt cttataatac ttcttttatc actcttaaag
    11221 catttttata gtatatcttt tctatatcat cttcactaaa cccttctttt tttagaggtt
    11281 catacagttt acccatctga gatatatctt caatctcaac ttttgaatct attccatcaa
    11341 aatctgaccc tagagataca acatctattc ctccaacatt tacaatatgt tttatgtgtc
    11401 taaccatatc ttcaagttta ctttcacttt tatcacttaa aaataagtgg aagaagttta
    11461 tacctgtaac tccaccttta tttgctaata ctttaatcat atcatcagtt aaatttctag
    11521 aatgattcat cattgctctt gaattagaat gtgtagcaat tattggttta gaagaaatct
    11581 ttgcaatttc ataaaatcca ccatctgaaa tatgagaaac atcaaccaac attcccaatt
    11641 cattcatctt atgtaccact tctcttccaa aatcagtaag ccctttttct ctatattcta
    11701 ctttattatg aggaaagctc aattcattca catgattcca acttattgtc atcattctaa
    11761 cccctaagtc gtaaaatttc tttaaatttt ctaatttacc ttcaagtact gcaccttcct
    11821 caatagataa aagagctgta agtttacctt ctgattggtt tttcataatt tcttcatagt
    11881 tagtagctaa agctatttca tcactatttt ttttcatttc ctcatgaaat ttatttgcca
    11941 tactcatgca ataatcaaaa ggatgtttta cttcttcagt atcaacaaaa agagcaaatg
    12001 tttgagctaa tgaatccccc tttttcaatc tgtctatatc aacagaatat ttattagact
    12061 taagttcact tgtttctaca ttttccatta attttgctat agtatcacaa tgtaaatcaa
    12121 taaatttcat atttataccc cctgtatatt ttatatttat atctaaaaat agaaatagtt
    12181 gtccttatag acaactattg acaactattt tttaatattc tttttaatat tacttaatct
    12241 tattcttctt ttaagttagg tattactttt ttaacttctt caatttgatg ccatgtttta
    12301 tctatattat tttcactaat atattttttt aaatcagtta aagatgtaaa atctttaact
    12361 gataaattgt catcatcatc tgcaaatctt aaaataaaca tgagactccc ttactttcca
    12421 caacatttct tatatttttt tccgcttcca catgggcatg aatcatttct tcctattttt
    12481 gcttctttaa ctattgtttt agacttctta taagccttta ttatttcttt tctttggtct
    12541 gctggtagta tatcttccca accttgtaaa ttatatagcc aatgagcctc aacagttacc
    12601 atattgttat ataatttttc ccaatctatt tttatagaaa cttctttatc agcttctact
    12661 tcttctaaaa ctatatcttc ttttaagctt tcacttattc catctatgaa tcccatgaaa
    12721 aattcattag tagttccaaa tttttcagcc aattcactta ctttaccttc aacaacatcc
    12781 attttattat ttaatatttc attgtatatt ccagcttcaa ctttcaaata ttcttcccag
    12841 aattttactt ctgcttcttg actttcatga ttatcactta aatttctcca ttcagtatat
    12901 aaactcatac tttttcctcc taagtatacc tatattttta tagtttttat agtataaaat
    12961 cttttagtta gattggaagt ttcttctttt tgattatacc atattctatt taaagttaac
    13021 tcttatatta tatcataatt aaacaattct tttaatactt ttatagcata ctcttggttt
    13081 ataaaataag ggcttgtcgc aattgttatt actttggctt ttttaattaa gttttttatc
    13141 ttgtaaaatt tgagttcaaa tggtatatag tccatatcac ttgaaaatat atccaaatct
    13201 atgtcaagaa caaactctga ttctacttct aaatcaaatc catatagact atctattatt
    13261 attaattctg aaaatatgtt atattttaaa gcaggtttta taaaacttcc cacatttaaa
    13321 acttcatttg tatatctaaa tacatctttt agattattta catctacatc atagttctca
    13381 ggttctctag tatccttatg ttggtctaca tgcactaact tgcatccttt tgtaaactca
    13441 ccctttaaca aagattttgc ccaaaaataa aatgcatggt tatgattgtc aaacacatat
    13501 atctttttat ttcccaggac atattcgacc atattctcca atccttttgc ttttatttcc
    13561 ttattttcat ctacttcatt aaatactacc tcattgccta accttacatc atttaatgta
    13621 ccatttatta gttttggaac gtatatgctc ttattttctc ttttatcata tgaaaataca
    13681 ttatttccta ctggtttttc tatataaaat ccactatatt cataattttc cataaaactt
    13741 tatttcctcc attgttatat aaatatataa gttataattt taaaaattaa aatgcactta
    13801 ttgtaaatca tatcatctat tatagaaaac atcttatata actaaatata atatcttctc
    13861 tcaatatgta ttatacaata agtgcaaaat atatttatat aaaatatact acattcctat
    13921 aacaaaacca tcagcttcaa ttggaaggta actaactctt ctattcaact ccacctttcc
    13981 aaaaaatact acccaatatg gtctaaaaat actttcaatt ttaactattt caatctctgg
    14041 gattcctccc ataaacctat gagttaatct catagccatt ttatttgcct ttgcttttac
    14101 tcttcgttcg tccttatcac tatactgcac aatattacaa tccacatttt ttacaacttc
    14161 atatgtagga aaatcttgta ctatactacc agaacctgta gaaccattca ctaaaatttt
    14221 taattcattt atatcattag aatatctcct attcttgaaa atggatttcg ttttgtgaat
    14281 tttaagtgtg ataattttaa actctatata atgaagtcta acttcatgag catatttgtt
    14341 ctttaatatt agcttactaa ataaagaatt ttttccactt ttagctatct ctatagcttc
    14401 ttcttttgta attcttatag gtaacgctct tatatccata aaatcattct ctttcttatt
    14461 tttttcttgt catttttaaa actctatata tataatcaaa aaatattaca attacaactg
    14521 ccataactcc atatggaatt atttttccaa ctataatatc atttctagtc aatataccat
    14581 aaaatataaa tcctgttatt aaaaagtaca ataatactag aaaaccgtct attccaagcc
    14641 attttatttc taaatctttt tttaccttag acactaattt agacatagca gtttctcctt
    14701 ttatagatta ttttgtgtat ttaaattttc acctattcta ttaccaatca tccatgcaat
    14761 aaatgaaaaa ggtatagcta tcacaagagg ctttaactca aagctctgaa ctggaaatat
    14821 tttaaaataa ccaaaagtca ctccaccaac aatcattcct aaaataccac ccatagatgt
    14881 tcttcttcca tttccaagaa gcccacctac tataggtaca aatgccgaac ccatccaaat
    14941 acttcctaca aatatacatg catcatatac attttcaaat ctaaatgcta cgctaagccc
    15001 cataactgct gctaaacata cacctattct agtaaacttt actaattgtt tctgagataa
    15061 ctcttttttt gtaattcttc catatatatc atttgctaat gtagccccag ctgctagata
    15121 atagctatcc aatgttgata ttatagaacc taaaattcct attataaata ttactcttat
    15181 tccttgtggt aatgtatcta aaactaaagt tatatatcca gcagctggag ccatttctgg
    15241 ataagtagct cttactataa gtcctgtcat agttaacacc atgtcaaaac atatccatag
    15301 tgaaaaacag gttaataagg ctcttcttcc aaccttagga gaatctgctg ctgaaaatct
    15361 ttgataaaaa gttgggtctg aataagctcc aaaagaatat acaattaaca tcaatgcttt
    15421 catagctgac ataccctttg tagggtgcat aagctgtggt gcatctttga gtgcttctgt
    15481 aagaccttga aaccctccta tttgttcaaa ttgacctggc ataaccatag ttaaaccaaa
    15541 taccatacaa aaaaacaata ccatatctgt aatggcaact cccataagcc caccaagtac
    15601 tgtaagtaca acgactactc ctaccattat taatcctatt aattctttat ctattttcca
    15661 tgctacgtta cctatagttc ccatagcagt tatttctcca agttgtgcac aatatataaa
    15721 catcaaaatt gccccaatta tagcaacatg cttgccataa aaactttcca gtaaatcagg
    15781 aactgttata ttggttctta tatgaatcct tggaccaacc cacaatgcta aaggcattct
    15841 cccaatatgt gcagctattg accaaactgc ccatgcagat attccatata tagccgcata
    15901 ctctacacta cctactgtac caactcctcc ataccatgat gcaaccaaag ttcctactat
    15961 taaggaccat ggtaaacttc gatttcctag gtaataactt tccatatctt ttacatttct
    16021 agaaaaataa taacctattc ctaataatat aaatgcaaat ataactataa tcccattttc
    16081 ccaaaatgtt aacatatcta atctccacat tcttatattt taatttttaa ctttaatatc
    16141 taaatatgaa caactctacc catttccata aaaataggta gagttatata ataattataa
    16201 gtatttaagt aatcttgtta ttaagcaatt ttattgttta aacctttatt ttctctttta
    16261 tttattttat ttaaagcata agtagcaaat atccctataa ctgcacatac tacagataaa
    16321 ataaatatat atgtatatcc tgtttgtcca tatttatcta accaattacc tgcaatagtg
    16381 taataaaacg catctggaga aaatcctata aatgaagcaa atccaactac tgctcctgta
    16441 gtttctaaag atatattaga ctctgaaaca gatgcaaagt atatacctct aaatccaaat
    16501 aaaattacac ttagtattat catgtttatt acagctatcc atataaatcc tgtactctta
    16561 ggaataacca agtaagcagc tgtacttact gtcattaata taaatcctac aaacattact
    16621 cttatagatg aaccaacttt atctgttata acaccagcaa ctggagatgc tcccattttt
    16681 ataacataag ttcttattat tgataaagca gaaactagag tcattggcat tacataaact
    16741 tctgataagt atggacttaa ataagttaaa cttgagaata ctaaataagt tgaaaatatt
    16801 ataagaccaa ttaaccaagc ctttggcatt gtaactactt ttaacaatga cttaatactt
    16861 aatccttcat ctgatgctcc actatctttt ttctcttcta ctaagaaaaa tagaagtata
    16921 ccacatatta tagatgctgc agaatataac catactacat atttgaatcc tactgttatt
    16981 tcagcaaatt taccaaagaa ataaagacct ataaaagaaa ttaatgtacc tgatacacct
    17041 gataaccctt cataaaaacc aaatattctt ccttgctctg actcacttcc ttgcattcta
    17101 acaaccttaa cagaggctga gaagaaggtt aatattgtcg taaatcccca taatacaaat
    17161 attaaaagta tcatactata tgatgggaat gtagagaaat aaaatccaag tattcctgaa
    17221 gcaattaaag aaaaagttat taattttcta actgagaatc tatctgcaaa ccatcctcct
    17281 ataaaataag ataccattcc tactaatcca tataaactca ataagttccc tatttgtgta
    17341 tgacttaatg ctaaagcttg ttgcattggg tcataaaatg atgatttcat atatggtaaa
    17401 ctatacatag ctgttgttcc aaatgccaat atgaatatta tcaaaaatct tttaacatta
    17461 tttttgcttt ccattgattc tctctcctta aaattttatt gtttataatc tcattcatat
    17521 aattttattt taatttttct attgcttgtt taaaatcatt tattatatca tttgaagatt
    17581 ctaatcctac agctattctt attagatttt ccccattcat ttgaactaat gtatgctctt
    17641 cccctaagct tgttgctatt gttgcaagct ctaaagaatt cattaatctt ctactagctt
    17701 ggtctcttgt caatccattg ataccatcct taagtttaaa tgaaactaca cctccaccta
    17761 aaccattcat ttgagtttta caaagctcat attgaggatg agattttaat gctggatact
    17821 ttaccaattc cacattctct tcttcctcta aaaattctgc tactgctaat ccattttcac
    17881 aatgcttttt catcctcata tctaacgttt tcatacccct taaaatcatc catgcactca
    17941 ttggtggtaa agatgctcct gtaaaacaac ataaacttgg ccatctaact aaatctatta
    18001 attcttttga acctgctact gctcccccta aagtatcccc atgtccattt ataaacttag
    18061 taagactatg tattactaaa tctgcaccta aatctattgg tctttgtaca attggtgtcc
    18121 caaaagtact atcaactata gctaagcttt tattttcatg agcaatttct gctattgttt
    18181 ttatatctac taagtctaat gatggattta aaggactttc tatataaact attttagtat
    18241 ttggtctcat tgcttctttt acattttgtg gatttactgc atctacaaat gtaacttcaa
    18301 ctccaaattt agataacaac tcagacatga ataatttagt gtgtgaaaat attccatctg
    18361 aactaactat atggtcacca ctttttacca atgctaatat agtagaggtt attgccccca
    18421 tccctgatgt actagatatg gcactttctg cattttcaag catagccaac ttagcttcta
    18481 actctgtaag agtaggatta cactctcttg tatacacata tccccatgtc atacacaact
    18541 cttcaaaatg ctctactgtc tttgcagcaa atgttgctgt ttgacatatt ggtgtagcca
    18601 atgcacctgt tgttgggtct gcactatgtc cccaatgtat tatttttgtc tctaaatttt
    18661 gattaatatt gttactcatt ttgatttcct cccattttga cttatttata caacgttttc
    18721 cctgccaaat ttcatcaatt tagcttagtc ttaatacatt ttttcaagta tactttttta
    18781 tttgttataa tttctaaaaa tatgacactt attttgtttt tttacagttc taattataaa
    18841 ctctatagta actatagagt caatatgttt ttgatatttt ttttacaatg tttaaaatag
    18901 tcaatacaaa aataggtata tcttagaata actaaagtca tcctaagata tacctatttt
    18961 tatatcacaa tataaataat tacatataag cattagtttt tttatcataa atagattttt
    19021 caagatttat cattttttca taaatatatt atagttaaat attttaatat tacctaacat
    19081 ctacaataaa atatactctt aaaataaaat tatataatta aaaatactct ataaaagcta
    19141 aaaagttttt ctattcgcta catctaaaaa taattagata gaggtataaa tgtatttagt
    19201 ttatttatac ctctagtctt ttataaatta tatctctact aaattgattt tttagcacga
    19261 ataagtgtgc tcttggagat accagtcatc ttcacaacct ctctatatgg cttagaattt
    19321 aataacccca atgcatattg tatctgttga ttgctgtatt tctttggcct accatcttta
    19381 aagtcctttt tctttttagc aaattctttt cctaattgag tacgttctat tatcatattt
    19441 ctctcaagtt ctgcaactgc aagtaatgta gtcaaaaaga atttacccat tgaagtattt
    19501 tctaaaaggc ctatatttaa aatatgaaca cttacttctt tagcaaataa accttctatt
    19561 agttgtattc cttctgatgt acttctagct agtctatcca gcttagttgc aacaagtaag
    19621 tctccctttt ttagagatga tataattttt tcaaatttag gcctgtttgt agtagtgcct
    19681 gtataagttt cctttattat tttagcatca ttatatttat tcaaaatttc ttgctgttgc
    19741 tgatacaaac tgttgttgtc tgtttgattt tttgtactta cacggcaata tccatatata
    19801 ataatcatca cttcctaaca taaagatttt gtacagaagt tacccagaag cttgttttta
    19861 cttatcttta tccattttac taaaacgatt tttgggtgtc aatactgtta acttttgaca
    19921 ccataataat tcctttataa tttgtttgac tatgtcaaaa attaccaata taatgaaatt
    19981 aatgagttta tacaaattgg aggaaataat atgaatagtc tgttaaaaga agcaatagat
    20041 gaattaaaaa acatccattg cggggaagtt tttttattaa aagaattgtt caaaggctat
    20101 gaatggaaca ggttggagat gaatatcaga ttgaaattag gaaaactttt tttgtatgaa
    20161 gtggtaacta atcccaattt aaatgtagag gtcatcaata aaaattctgc ttatcagcaa
    20221 atgtataaaa aaaaatagtg acttaaatgt atataatagt ataatcttct tattgattca
    20281 aaagttaaaa cttttaatac aaaacaatca ttctatttta acatttatca atatgtctaa
    20341 atttatataa ttaacacata cgaatagtat tgtttaaatc acattattta agatagataa
    20401 atcttaaccc tactccagca taaatactaa aatagggctt agaattatct atatttttat
    20461 cccataaaat gcttagctct tcctcttgat tgaacatata tatctgggga aatacaatag
    20521 aagcgtaaat ttataaaatt tccaaagtca gaaaacttca gttcaaactt aggtatacca
    20581 aaactttcag gtgctatttc gtcaacacca aaatagatta taattccatt atctgtaaga
    20641 taaaatgctt ggtcttcagg tataactaac tcaacattag aataatacat ttctctattt
    20701 tcatttattt gagatttaat gtaatcagta attatattaa tataatcaac tccatctcta
    20761 aaaatgtcat taagtaaaag ttcttttcca gttgtcaaat ctatattgta attattcaat
    20821 gtattgtatt taggcccata gttattagca aaccccataa ggtttaatat catactcagt
    20881 acataatttt tattaaaagt aactgcgtaa ctggtagata cccaatagct ttgttttgga
    20941 agtgaatttt gaggagctgc gttattatat tctcgttctt cttcctgtaa gccattttta
    21001 aaattattta cagtcattct tacagcattg tttatgttat ttaaaataac tgggtttaca
    21061 tacgtggatt gatttctaat attgtatata acaggatact ttacatcata tgaaaaatac
    21121 ttacttcttt caaaatcttc attaaattgt acatataata tattatatgt acaggtattt
    21181 gtagattgat aattttccat taaaatcctc ccccttttat aaaaaaatca gatacaatat
    21241 aatatgctta aaacagtaat aatgttaatg gtaaatgtaa agaatagaaa gtatctaaaa
    21301 cagcaaaaaa tgactaattt tgtggtataa tacataggat tgagagttat tttataggag
    21361 gaaaaatatg ttattattag cagataaatg gaaagattat gaacttatag atatgggaaa
    21421 tggagaaaaa ttagaacgtt ggggaagtta tgtacttaga agaccagacc cacaggttgt
    21481 atggccaatg gaatctgaat gggctttatg gaaaaaccct catggacatt atcacagaag
    21541 caataaaggt ggaggtcaat gggaacacaa aaaaagatat ccagaacaat ggactataaa
    21601 ttataaaaac ttaaagtttc acattaaacc aactggcttt aaacatactg gattattccc
    21661 agagcaagca gctaactggg attggatgat agagaaaata aagaaagcta acagacctat
    21721 aaaagtgttg aatctttttg catatacagg tggagctact gtagcatgtg catctgctgg
    21781 tgcagaagta tgtcatgttg atgctgcaaa aggtatggtt aattgggcaa aagaaaatat
    21841 agaattatca ggtcttaaaa atcaaacagt aagatttata gtggatgatg ttgtaaagtt
    21901 tgttgaaaga gaaataagaa gaggtaaaaa atatgatgca ataataatgg accctccttc
    21961 ttatggaaga ggtcctaaag gagaagtatg gcaaatagaa gaaaagttat acagttttgt
    22021 agatttatgt atgggagttt tatcagataa ccctttattt ttcttaatta attcttatac
    22081 aacaggattt tctccaatag ttcttgaaaa tgtattgtct acaacaatag gtaaaaagac
    22141 aaatacagga gatatatacg gtggggaatt aggaatacca gcttccagag atggtaagat
    22201 acttccttgt ggtatatttg gaagatggga gaaaaaataa tgataaaagt catatatgaa
    22261 gataaccatt tattagttgt agaaaaacca gttaacatat tgtctcaagg agataatact
    22321 aatgataaag atatggtaaa tcttttaaaa gactatatta aaaaaaagta taacaaacct
    22381 ggaaatgtat atattggttt aatacataga ttggatagac cagtaggtgg agttatggtg
    22441 tttgcaaaaa catcaaaagc agcatctaga ttgtctgagc aggtaagaag taagactttc
    22501 aaaaaaactt atctagctgt tgttcatggc aagataaaat ctaactcaga tatactaaaa
    22561 gattttttat ataaaaataa aaaaactaat atggtaactg tagtgaaaaa aagccataaa
    22621 gatgccaaag aagctgaact taactataat gtattagatt ttaaagaaaa tctaagttta
    22681 gttcaagtta atttaaagac tggtcgttct catcaaataa gagttcaatt tgcaagcaga
    22741 aaacatccat tatacggaga ccaaagatat ggcaaagaaa taaataagat tggacaacaa
    22801 atatctcttt ggtccaataa gatagaaatt tgccatccta ctacaaaaga aaaaatggaa
    22861 tttacatgtc atccacctga caaatatcca tggaatatat tttaaatctc aaatgaggtg
    22921 attatatgag taaaaaattt tcaataggag aaatgtctaa acttcataaa ataagcatac
    22981 agacactaag atactatgac caaatagatt tatttaaacc tgttcatgtt gaccaagaaa
    23041 gcaattacag atattactca attgaccaat tttcacaatt agatattata aaatatttaa
    23101 aatatttagg tatgccttta aaagaaatta agtcaaagct tgatgaaaat aatgaagaaa
    23161 ttctaaaatt actaacaaat aaaattaata tagtggatga aaaaattaaa gaattagagc
    23221 ttataaagaa ggtactaagc acaaagaaag aaactatgga actttccaga agacaaatag
    23281 caaatatatt agaagataat atatctgtgt tctatggtgg agtaagtgga cttgtatcta
    23341 tagataaatt tttaaaagaa gataaagtta tttatgacaa tagttttgtt actgttgaaa
    23401 gagacttatt tgatataaaa tcacaaaaac atataacaga aatttcatct ggtgatttta
    23461 tttgtatgac atataaaggg ccttacaaag aaaattatac cgcttttaag aaactggttg
    23521 attatgttga atataaaaga atacctattg aaaattatat atatgaaata cctataatag
    23581 accctctgtc tacaaacaac gaagaggaac ttctaacaga aattcaaata caagttaaaa
    23641 agagagaaat tgacttaaga tgtctagtgt agtaatctag tttacttaca catcttaata
    23701 tagttgtcta ttatcaacat atgatttttc tagatagtga ttcctaacag tgagattttc
    23761 aaaaaatagg aggaaatatg gctaatgcta attccctcct atttttaatt aatccatatc
    23821 attttaatta agccatgtca tcacgaagtg catcaataat attttctttt tttatcttat
    23881 taatagtata caacattgta ataaatacta taaataaaac actaaatata ctaattccaa
    23941 tactaaacca tggaatcaca aaatcaatat catctgtaac cattacttta tgaattagcc
    24001 atgaagaaat tactgctata ggaagtccaa agaacaatgc tctcatgcca taaaaggcac
    24061 actcaaaatt catcatcttt tggaaatcat gctcagacat tcctacagag cgaagcatgg
    24121 caagttctct acgacgtaac ttaatattag ttgaaattgt attaaacaca ttggcaatcg
    24181 aaatcagaga aatcataatt atgaaagtat aggcaaaaac attggcaata aaaatgtaat
    24241 tacggttctc atccatcatt ccatacatat tgtacagatt gtactcattc gtaactccct
    24301 cactctcaat aatttgctcc atttctgcaa ctgactgagt aggatttttc gattgaaagc
    24361 ttaaactttc aattcctgta gaaacaccag gaatctcaaa cttatctttg gctgaataag
    24421 gagccaatac tctgaagctt gctttcttca catctgaact tctgattcct ggaggagaat
    24481 caggtggcac tactttgaca aatgaaatat tcacattttg tctctcaggt atctccaact
    24541 tgccattagt ttcaggagtg aggctaacat tcatagaaga actgttgaaa atatcaataa
    24601 tattattatc ctttgttacc atagaaacag caatcagttt tgcattttgt ccagtatatt
    24661 cttctgctgg taatccaaga cttttaataa tattcagata agttctatca tcaagaaatt
    24721 gcatatctac tggtaattca agcatttcac tcatagaatg tgaaccctca tagttcaaat
    24781 aatcgtctgt aagctcactt actttggcaa cacatgaata ggtcataagt ttctgataca
    24841 aaccttcgta aacaccattt gcagttttta gcttatcgta aagcttcaac atttcgttgt
    24901 ctttcatacc ttgtgtagaa aaaataatat cctggtcagt aacctctgac ctctgatttg
    24961 acatctgttt taggtctgct actaaagcac ttgtagatat aaatagaact atgcttaaaa
    25021 caagcgacaa tacaatactt cgataacgtt tcttgtttct tttaaagttt ttcatagcaa
    25081 gaactccctc aagaccataa actcgctgtg ttagttttga tgtcttcaca gatttagatt
    25141 caatttttac ctcatttgtc tgacgaatac actccatcac tggtgtgttg gcagcctttc
    25201 ttgctggtat gtaagcagaa attaagattg taaccatact taccactgct gcaacgatga
    25261 ttgcagggat agacaatgtt aatgtcaacg gaacgtttac atagagaata tttgcaaagt
    25321 ttttagctat gacagaaatc acaagtccaa tactgactat accgattgca acaccaattg
    25381 gtataccaat cgcaccaatg caaattcctt caaatagcac cgaatttcgt aactgttttg
    25441 ctgtagctcc aacagatgaa agtattccaa attgatgtgt tcgctcgttc aatgacatgt
    25501 taaatgaatt atatattaga aaaattgagc ctagcatgat tatggcaagc acaatgccac
    25561 caaccgagta caaaaacata ttgaacaaat tgttatcaga aacacctatg aagcgcaaca
    25621 cattgtcatt gaaaatggaa gcatggtctt tagtgctact tgtgtaagag cgaatctgat
    25681 gtggattttt caatgtgaca aaaaggctaa gactgtcagt cttgacttgt ttatcttctt
    25741 tggttatcaa ggtatatcct ggtgcagaag attcttcaaa tacgggcttt ctacagatac
    25801 ccacaactgt ataagttttc ttatcctgtg gcacaaaaac ttcactccca gcagtgtatg
    25861 ggtcactctg accaagcttt tcattttcac tcattcgttt ccctacatcc aaagaaatag
    25921 tatcacctac tgcaaatttg acaccaccat ctgtcgcaac tttacttgaa acaagaatct
    25981 ctctactatt ttcaggtaat cttccagaaa ttaaagttat aggcaaagta tcaaaagctt
    26041 ctttgctaaa tccagcaata aaaagatatg gtttatctgg atttctgtta ccttcaagtg
    26101 ttgcatagcc aatattttca aatgttgcag tatttgcaac ttcatcattg actctttgtt
    26161 cctgtacaaa agaagaatcc acatccaaaa actggacatg ccaatcacca tattttataa
    26221 tagctccatt tgtcatatag ttcatcaaag aaactccaaa ggtagtaact gctgtaatca
    26281 tagcagctga cagaacaacg cctataattg ttacaagagt tcgagtacga ttctttttca
    26341 atccttgcag agcaactttg ttaaaaatgt tcattaacga ttcaccttcc tctcatcacg
    26401 cactactttt ccatctgaaa tacagataat tctatctgcc tgtaaagcaa tgttttcatc
    26461 atgtgtgaca ataatgagag tttggtgata tttactatgg ctctctttca gtaactggat
    26521 aatctcttgt ccatttttac tgtctaagct acctgtaggc tcatcagcaa gcataactgc
    26581 tggtgcattc atcaatgcac gaccaatggc aacacgttgc tgttggccac cagaaagttg
    26641 atttggtaaa tgtgtttttc gttcttttaa accaagtaat tctaacaagt catttaaccg
    26701 ttcttgattg accttacgct tatccataag tataggcaat gtgatatttt ccactacatt
    26761 taatgtgggt atcagattgt gaaactgata aatcaatcca acttgtcgcc tacgaaaaat
    26821 agcaagtttt tcattgcttt ggccatatac atcttgcccc tccagatata tctttccact
    26881 tgttggtacg tctactcctg caatggaatg tagtagtgta gatttaccag aaccagaaga
    26941 accaatgatt gcagtaaact cccctttttc aatagtaagt gagacatggt caagtgcagt
    27001 aaccttattt tcattcttac cataaacctt acataaatct tcaatcttta aaaacgccat
    27061 tatataagtc tcctttcata ttttataaat atatcataga actttcaact tacatttaag
    27121 tgacatttaa atgagagatt cgtcactttg gaaaacgaat aacaaatatt gcaccacctt
    27181 gtgggtgatt ttgtgcagta attgttcctc cttgtctcat aataatcgtc ttacaaagtg
    27241 ccaatcctat tccataaccg gctgtgcttg aatttttccc acgataaaat ctgttaaata
    27301 gacatggtaa atcttctttt ttaaagcctg ctccgttgtc atgaatagta agttgagtaa
    27361 acaaaaagtt gtcctcacaa atgatatcaa tttttccatt atcacccaca ctctccatgc
    27421 agtttttaaa tatattttga attgcttctg aaagccaatt taaatcaccc aaaatcctta
    27481 ctccttttgg tatatcaata tgtaaagtga tattgtgcag ttccatggga atcaaaagtt
    27541 gatgaagtgc agaactaatc aaattattca catcaatttg ttctttttga aagactacaa
    27601 tacctgcatc taaacgtgat aattttaaaa gggaagtaat cagccagtcc attcgtataa
    27661 gtaattcctc tgtttctcgt atcaactctt ttctctcatc ttcatcagaa gtattctcta
    27721 acagtgacaa aataagagtt actgatgtaa gaggagtgcg aagctggtgg gctatgtcag
    27781 ctagtgagtc agcaaggtgt tctttttctt ttttcagtgc gtaattttgc tctctaatgc
    27841 gtaacgtcat tttagttatt tcactttgta gaatagaaag ctcaccctca tctgattcag
    27901 caatatacag atggttagca ttatgaagca caagattaat ttgttctgaa atctgtgcaa
    27961 tacttttgta tcgattttta gtaaacacaa aaaatgctat tccaaaggta gtggcagaaa
    28021 caatagaaag tattccagtc ataatactga ttgcaaatcc cagggctaca gtgagaatag
    28081 taattaataa gaataaaata gcaaattttt gaaactctct atttcgaaac ataacttccc
    28141 cccaatctat accctgtccc acgaacagtc aaaattattt gtggacttga agggtcatct
    28201 tctatcttct ctcgcaagcg tttgatgtac acagtcaatg tgttgtcatt aacaaactca
    28261 cctgccgcat cccacaattc atcaagtaat cttaccctcg taataatact tttaggattg
    28321 ctaataaaca ccaataacaa acggtattct aaagctgaaa gaaaaacctc attgccattc
    28381 tttttcacaa caccggttgc tgtatctaca taaagcccac aaatttcaaa agttgaccca
    28441 aaacgtccac tttttcgtag tgctgttcta attcgcgcaa tcagttcacg tggacgaaaa
    28501 ggtttagtaa tataatcatc tgcacccata ttcagtccag taacaacact tgattcatca
    28561 ccagaagctg tcaaaaagat aacgggaatg tcttgctttt ctttgatttc tgtgcaaatc
    28621 gtaaacccat ttccatcagg taaagaaata tcaataagtg ccaaatcaaa tttattctta
    28681 gcaatctcta caagtgcttc acttcttgtg gatacatggg ttactataaa tccttctgaa
    28741 ttaagcaaaa gaataagatt tttggcaatt gctttatcat cctcaactaa aaatatccgt
    28801 ttcatttact ttcatcctcc tttattttat tagttttata tattccagca gagcaatagt
    28861 acactactat tttacttatc attacacaat tttcagtagt ttcttttgtt cttatataat
    28921 ccatgcataa tacctccatt gatttattat atttcttata acataaatac acaagataag
    28981 acaaaaaaca taatgtaagt gcaagttttt taactcacta acttataaac atacactcct
    29041 actaacatta ctttcttgat gacacatctt acaagcttat attcatacat caatgaaaaa
    29101 ttaaatttat ccatttatta aaataaaagg caatagcttc tatagctaat gccctttatc
    29161 ttaaaatgat ttaaatacta tacaattttt ataaatggct ataaataaac tcgtatagaa
    29221 tataaaattt atctacagtc ttttgttata caaaatttat aacctcttat agaacaaatt
    29281 taccactttg caaacttatc acttttactc agctatatta taaactttta atttatcttt
    29341 gtttaaatgt ttcttcttaa cttctactaa agcttttagc gtatccaaag atgtcattat
    29401 ttcagtagaa aattcaatgg ctattcttct catcttaaat ccatctcttg tagagtcatt
    29461 tcctttagtt ggtgtattta ttactatatc aacttgttta tttcttatag catctaatat
    29521 gtttggtcta ggttcttcaa ttctatttac tatatcagcc tctattccat tttctcttaa
    29581 actctt
//
LOCUS       DBGJFF010000039        28693 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.039, whole genome shotgun sequence.
ACCESSION   DBGJFF010000039 DBGJFF010000000
VERSION     DBGJFF010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28693
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.039"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            170..1621
                     /locus_tag="ACOIAF_001763"
     CDS             170..1621
                     /locus_tag="ACOIAF_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HGZ2668776.1"
                     /translation="MDFFNGILTSINNAVVYVNNYLWSYILIIMLVVIGIYFTIKTNF
                     VQFRYFIEMFRLLGDGTANKNAKKEGKISSFQAFCISTSSRVGTGNIAGIAIAVVAGG
                     PGAVFWMWLIALIGSASSFVESTLAQIYKVKDGQAFRGGPAYYMEQGLNKKWMGILFS
                     ILITICYGFVFNAVQANTVSLAFNNAFGISKMAMGIGLGILTAIVIFGGVHRVAKVSE
                     IIVPVFAGLYILVALAIVVMNITEIPSVIALIVESAFDFKGMAIGTFMGVVMTGVKRG
                     LFSNEAGMGSAPNAAATAHVTHPVKQGLIQSLGVFTDTIIICSCTAFMVLLYSGYSSS
                     GATGIELAQEALTHHIGPVGNIFIAVCIFLFAFSSIVGNYYYGESNMEFMSGSKTKLN
                     VFRVFVVGMVLFGSLTQVDVVWNLADLFMALMAIINLIAIALLGKYAFIALKDYTSQK
                     KSGIKDPVFIADEIEGLEKVSEWHRELSEEHLG"
     gene            complement(1819..2472)
                     /locus_tag="ACOIAF_001764"
     CDS             complement(1819..2472)
                     /locus_tag="ACOIAF_001764"
                     /inference="COORDINATES: protein motif:HMM:NF019947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HGZ2668777.1"
                     /translation="MTFNMTDNYIFDSYNNLVDGLSAYLGEGYEFILYKFNGVHYSVN
                     KIANGKYNTVKEGDIITDFDLALINKLENDADGDFISYFNRNKLGEPLKSTDIVIRGV
                     DDKIIGLLCIHFYLNTPLTRIIGSYIPEHNAMQSAPVLENIVEDTDEVILKSYQDAKV
                     LVENDSNISSNDKNKSIVSILNESGIFKIKDSVVKVADLMGLSKNTIYMHLRNLNKN"
     gene            2934..4313
                     /locus_tag="ACOIAF_001765"
     CDS             2934..4313
                     /locus_tag="ACOIAF_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HGZ2668778.1"
                     /translation="MAQDKIVVEILESIGGKENIKEVAHCMTRLRIKVCDSSQVNQEK
                     LKKIKGVLGLVLKGEQYQIVIGPNVSQVYNTLNEVIGIIPEKDSKKTISEGKQNSIAK
                     ILDTIAGIFNPIVPALAGAGLIKAILALLVASKFLSADSETYIILNTISDGVFSFLPF
                     MLAYSAAKIFKMNTYVAVAIAGAMLHPNFTNLIQQGLTKISFIGIPMSLIQYNGSVFP
                     IVLTIWFASYIEKGVDKITPKALKIILVPALTILFAAPIALIIIGPIAQMLGNYIAGG
                     ASILFERGGIFAGLIYGGIYSFMVVTGLHHGMVPVLVDGITRYGFNYISPVSGSSNMA
                     QAGSAFGVWLKAKDKDLKAVAATATISALTGVTEPAIYGVNLRLKKPFLCAAIGGAAG
                     GGFAAYFQCKAFAMGGPSFLTLPMFIGEGGHVGLVAIAFTIAFIVSAIATYIVGFEEE
                     VTADSHANL"
     gene            4357..5091
                     /locus_tag="ACOIAF_001766"
     CDS             4357..5091
                     /locus_tag="ACOIAF_001766"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: protein motif:HMM:NF009022.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HGZ2668779.1"
                     /translation="MKIYLTKDYEEMSQVAAQMVLKYMYSNDNKRINLSITAGQTPKR
                     TYEILSEFVKNKPYFENVHYYNFDEIPIKGEKVGVTIRNLTNMYFREAQIPEERIHVL
                     DENNYQGFDRKIEADGGIDMVLMGLGWDGHFCGNLSGTVNSFDEETRAVSTYITDDMA
                     KLLTKSVGGDPEKLFDYYVTYGPKTIMHCKNIIMIVNGKHKAQILKQTLFGPLDLLCP
                     SSLLRLHPNFTLIVDEEAASEIKDLI"
     gene            5180..6379
                     /locus_tag="ACOIAF_001767"
     CDS             5180..6379
                     /locus_tag="ACOIAF_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HGZ2668780.1"
                     /translation="MYNHMKKVFQGLEGGLFSSVEKADVGDSYQKLGEQGVSLMGWAD
                     PFFPDYCIPKHILDAATLAISSNMSTHYTAPIGNDLLKIEIAKKLKEVNNLDVDFKRN
                     ILITPGSDSGLYYSMLPFINEGDEVLMPTPCYPNNIQNTKLMGGVPIFVELKESDGYQ
                     IDITEFEKRLTNKTKLVVLTHPNNPTTTIFNKESLEKLAKFIIDNDLILICDQAFEDF
                     TFENELITPASLEGMFERTVTIFSTSKGMGLSGFRVAYMVCSDKLMDKYYGAAVTVLG
                     ATNTVAQMSTIEAFKDRSFMEDFERVFDKRRQEAYKIINEIPNVSMQMPESGFLGWIN
                     VSKLGTSSEIQKYLVDEAKVCINDGINYGPGGEGHLRIILGSYKDDSKVIGALNRIKD
                     ALIKKQQ"
     gene            6403..6885
                     /locus_tag="ACOIAF_001768"
     CDS             6403..6885
                     /locus_tag="ACOIAF_001768"
                     /inference="COORDINATES: protein motif:HMM:NF012578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HGZ2668781.1"
                     /translation="MLSIFKSKKNDNKLKAFASGKVIPIEDVDDNVFSSKMLGDGIAI
                     YPTNEYVVAPCDAEVNIIMKDTKHAVGIKLFNGMEILFHVGIDTVSMNGEGFEIFVSP
                     GDRIKRGDKLISFNSKLIKEEGLQDVVIMVVTNSEQYKNIKYHIGLEAVIDDTIIAEI
                     "
     gene            complement(7200..7310)
                     /locus_tag="ACOIAF_001769"
     CDS             complement(7200..7310)
                     /locus_tag="ACOIAF_001769"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668782.1"
                     /translation="MDAIHYKVKIDGKIINKAAYTAICMNLEGVKDVLGI"
     gene            complement(7430..7726)
                     /locus_tag="ACOIAF_001770"
     CDS             complement(7430..7726)
                     /locus_tag="ACOIAF_001770"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGZ2668783.1"
                     /translation="MLKDLLASTIHKTLESEIEDHLMYERYDNQSKATSNSRNGYRAK
                     NVKSDFGEVKLNIPRDDFKPRVIQNYENEISGIENQVIGMYSKGMSTRDIYHII"
     gene            complement(8045..8473)
                     /locus_tag="ACOIAF_001771"
     CDS             complement(8045..8473)
                     /locus_tag="ACOIAF_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668784.1"
                     /translation="MKIHSIQNLTLDTRNLIKKYSLTLNEDLIWEFKHGKYHTIKYFT
                     HKFAVKESVLALLFNIHKLCYAKIKYFESNFSKYEPYKYSYQNEFVKCEMYDMNFMFH
                     KYSNIMIDLRNLQEIKTIEEFRLFCDYLESFEDNLQDLII"
     gene            complement(8874..9335)
                     /gene="ribE"
                     /locus_tag="ACOIAF_001772"
     CDS             complement(8874..9335)
                     /gene="ribE"
                     /locus_tag="ACOIAF_001772"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HGZ2668785.1"
                     /translation="MIYEGKLIGKDLKIGIINSRFNEFITSKLLSGAEDCLLRHDVSP
                     ENIEIVWVPGAFEIPLVAQKMAKSGKYDAIICLGCVIRGATSHYDYVCSEVSKGIAKV
                     SLDSELPVIFGIVTTENIEQAIERAGTKAGNKGYDCAMNALEMANLFKSLD"
     gene            complement(9440..10633)
                     /locus_tag="ACOIAF_001773"
     CDS             complement(9440..10633)
                     /locus_tag="ACOIAF_001773"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HGZ2668786.1"
                     /translation="MFNTIEEAIEDIKNGKMVIVVDDESRENEGDLLMAAEMATPESI
                     NFMATYGRGLICLPATEKKFKSLNIPLMVRENTDTFQTAFTVTIDGADTLTGISAYER
                     AETVKLFCDENSTSKDFKTPGHIFPLIAKTGGVLVRDGHTEASVDLARLAGFKEIGLI
                     CEIMKDDGTMARVDDLMIFKEKHNLKIITIKDLIEYRKINETTIEKVSSAFLPTKYGN
                     FEIIGYRDTYSNEEHIALTYGNINIENTLVRLHSECLTGDVFHSLKCDCGLQLESSMK
                     KIVENGSGVLIYMKQEGRGIGLLNKIKAYKLQEEGYDTVEANLMLGFEEDMRDFYMAA
                     QILKNLNIKSINLLSNNPDKINQLEKYGIKIENRIPINEEINDFNKLYLKTKKDKMGH
                     LLDII"
     gene            complement(10656..11321)
                     /locus_tag="ACOIAF_001774"
     CDS             complement(10656..11321)
                     /locus_tag="ACOIAF_001774"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HGZ2668787.1"
                     /translation="MFTGIVEEVGILRKITANGKSGKVTILSNKILDGTNLGDSIAVN
                     GVCLTVSNLGKNEFTADVMMETIRSTNLGLLNANDKVNLERAMSLSSRFGGHIVTGHV
                     DGKGTICKFEKDENAVLVSIRPDKKLLSSMILKGSVAIDGVSLTISYLDDEIFKVSII
                     PHTKINTILLTKNVGDFVNLESDVIGKYVNNFMANNYKELNSNSSNHKSNIDKDFLFK
                     NGF"
     gene            complement(11355..12467)
                     /gene="ribD"
                     /locus_tag="ACOIAF_001775"
     CDS             complement(11355..12467)
                     /gene="ribD"
                     /locus_tag="ACOIAF_001775"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HGZ2668788.1"
                     /translation="MNFVNQKEKDIYYMKKAIELAKNGEGFVNPNPLVGCVIVKDSNI
                     IGKGYHEKFGSNHAEVNAINSAKQSLKDSTLYVNLEPCSHYGKTPPCVDKIIQNKIKR
                     VVISTLDPNPLVCGNGVKKLKDNNIDVTVGILEDEARDLNEAFFYHIKNKRPLCIVKS
                     AVSLDGKIATKSLESKWISNESSRYLTHKYRNKYQSIMVGINTVLNDNPLLTCRLNQE
                     KVSHPTRIVIDTHLKLPLNSNLVKDKTSKTIVFTCCKESIKLSMLKENNVETIISPSK
                     NNLVDLEFVMYKLGELNIDSVLVEGGATLNDSLFRNKLVDKVKLFLSPKIIGGKDAPT
                     FVSGEGINHLSDSTQLTINNVTLIDGDILIESDVLN"
     regulatory      complement(12599..12720)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            12909..15392
                     /gene="recQ"
                     /locus_tag="ACOIAF_001776"
     CDS             12909..15392
                     /gene="recQ"
                     /locus_tag="ACOIAF_001776"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HGZ2668789.1"
                     /translation="MNKKLLEILSKYYGYTSFRKGQESIINSILSEKDVLAIMPTGGG
                     KSICYQIPALILDGMTIVISPLISLMKDQVDALKTMGISAAFINSSLSSKEFNEILNN
                     IRKNNYKILYIAPERLDAQEFIELINNNNVSQVAIDEAHCVSQWGHDFRLSYRRISDF
                     IKNLPKRPIVTAFTATASEEVRTDIINLLCLENPDYYITGFDRENLSINIVKSSSKNK
                     YILDYIQNHKNESGIIYVATRKEVENISNGLSKRNISVSKYHAGLSKNERSKNQEDFI
                     NDNVDIMVATNAFGMGIDKPNIRWVIHYNMPQSIENYYQEIGRAGRDGEKSECVLLFS
                     PGDVHIQKYLIDIGVENPERKLFQHKKLQYMIDLVYSNSCYRKTILSYFGENYIDNCN
                     NCSNCLVEGEIVDKTIDAQKVISCVARMKRSYGVTTIVDVLRGSKNKKILQLGLNTLS
                     TYNIMRDYSSEDLKNFVNTLVSHGFLDLVETLGNGNRGSFPTIRLNEMSMKVLKGEVK
                     VEFKEIVMTKSLEVEDELYNALRELRHSIASEERIAPYMVFGDGTLRAMSSSYPINKE
                     EMLDISGVGEIKYQKYGRDFEAVIKEYVEKNNINKKIDEKNVDSKNVNSDFLEVNTDK
                     VLYEKLLVIREEYATKEKLPAYMILTNKALKEISGRYPLDEEQLKDISGIGGVKFEKY
                     GASILAEVNKYVKENNINVIWEKKGKRKLILDGESRKNNEIALDLLNQGKDIQSISQE
                     IEVSLSTIIGYVHDYVKDGHSISFELGLENFYNEDKKKMILEVCEKVGYDNLNNIKRK
                     LPDSIKYENIRAVILDSYLENLKNNIIAE"
     regulatory      15481..15592
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            15734..17035
                     /gene="thiC"
                     /locus_tag="ACOIAF_001777"
     CDS             15734..17035
                     /gene="thiC"
                     /locus_tag="ACOIAF_001777"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HGZ2668790.1"
                     /translation="MNYTTQMDAARKGIITKEMEIVSQKEQVDVNELRELIANGQVVI
                     PANKNHKSLSAEGVGKNLRTKINVNLGISRDCKDIEKELEKVRVAIDMKAEAIMDLSN
                     YGKTREFREKVVEMSPAMIGSVPMYDAVGYLEKELKDITEEEFLNVIRQHAIDGVDFI
                     TIHAGLTRSVCQKIKNHERLTHIVSRGGSLLFAWMELNNKENPIYTNFDKILDICEEY
                     DVTLSLGDACRPGCIKDSTDGVQIQELVVLGELTKRAWERNVQVMIEGPGHMAIDEIE
                     ANVVLEKRLCHGAPFYVLGPLVTDIAPGYDHITSAIGGALACAKGVDFLCYVTPAEHL
                     RLPNLDDMKEGIIAAKIAAHAGDIAKNVKGAREWDNKMSKARADLDWCEMFRLAIDPE
                     KAKRYRDESTPTHEDSCTMCGKMCSMRTVKKILNNEELNLI"
     gene            17089..17283
                     /gene="thiS"
                     /locus_tag="ACOIAF_001778"
     CDS             17089..17283
                     /gene="thiS"
                     /locus_tag="ACOIAF_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HGZ2668791.1"
                     /translation="MKVNGKEIEFEKDLTVIDLLNKYNLKSDRVVVEVNLEIIEESNY
                     NTYVLKDEDIVELINFIGGG"
     gene            17276..18079
                     /gene="thiF"
                     /locus_tag="ACOIAF_001779"
     CDS             17276..18079
                     /gene="thiF"
                     /locus_tag="ACOIAF_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiF"
                     /protein_id="HGZ2668792.1"
                     /translation="MVKIKIFVNELLTDVEENTTAYKVRDSYNNKCDVLVLNGYPIKT
                     DMPLCENDKLTLIQKGVKPSLDELESLLIARHTPNIHNKLKKGKVAILGLGGLGSNIA
                     ISLARIGVGELLLIDYDVVEPSNLNRQQYFIDDIGKLKTDAMIENIKKINPFIKLNKR
                     DIFLNKNNMDTIKDSDLIIEAFDDASCKAQVCNYVLINLKDKYLIASSGMAGYYDSNI
                     ITTKKIKDRFYICGDFVNEAKFGEGLMAPRVAICANHMANLATQILIDM"
     gene            18142..18912
                     /locus_tag="ACOIAF_001780"
     CDS             18142..18912
                     /locus_tag="ACOIAF_001780"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HGZ2668793.1"
                     /translation="MDKLVLGGHEFNSRLLVGTGKYGSNNILPEVIKESGSEIITMAL
                     RRVDLDNKQENILTYIPKEMTILPNTSGATNAEEAVRIARISRKMGCGDFIKIEVISD
                     TRYLLPDNEETIKATKILADEGFIVLPYMTPDLYAGRRLIEANAAAVMPLGAPIGSNR
                     GLQMKEMIRIMIDELDIPIIVDAGIGKPSQAMEAMEMGADAVLVNTAIASAGDPVQMA
                     RAFKLAVEGGREAYIAKTGNVSEFANASSPLTGFLGNL"
     gene            18929..20032
                     /gene="thiH"
                     /locus_tag="ACOIAF_001781"
     CDS             18929..20032
                     /gene="thiH"
                     /locus_tag="ACOIAF_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HGZ2668794.1"
                     /translation="MSFYDVIEKYRDFDFDGYLNNVTDNDVLRSLSKDKLEDFDILNL
                     LSKTAVKHLEDMAQKAHKLSVQYFGKTVCLYTPMYIANYCVNQCVYCSYNIKSGIKRK
                     KLTMDEIREEGEAISKEGFKHLLVLTGESSFHSSVEYIGEAIEILREKFPSIGIEVYP
                     MEVEEYKYIVDKGVEGLTVYQETYDEEIYKRVHIKGPKSNYKFRLDAPERGAKAGMRT
                     LSIGALLGLNDFRKETFFTILHGKYLKTKYPHIELSYSTPRMRPFKGCFEELVDISDT
                     DLVQAMVCMRLFDPHAAINISTRENLEMRSHIIPLGVTKLSAGVSTDVGGHSQDEHDT
                     AQFKINDESTVKDVEKMLNSIGYQHVFKDWERF"
     gene            20033..20722
                     /locus_tag="ACOIAF_001782"
     CDS             20033..20722
                     /locus_tag="ACOIAF_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HGZ2668795.1"
                     /translation="MYLITNRKLCSEERYLEVIKESILSGVENIIIREKDLEYQELRK
                     LYMKIKTKINCIDFQEQISDESLKTNINQKECRNKFKVNFIINSNIEFFEKVDCQGIH
                     LPFKLFLNLIENKYNFNENKILGLSLHKVEEVDYLEKLIRNQNIKIDYITLSHIYETK
                     CKEGLNPKGIELLKEAKKITDIKIIALGGILPSNVKETLKYCDDFAIMSTIMRSKDIK
                     KTISNYNEKLN"
     gene            20910..22304
                     /locus_tag="ACOIAF_001783"
     CDS             20910..22304
                     /locus_tag="ACOIAF_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HGZ2668796.1"
                     /translation="MTDSKKKRFIIPHTFTIIFLLIVLMAILTWIVPSGEFSREDVEG
                     RMVVVPGTYQSVESNPQGFADVFKAPIEGFIDSAEVVGFVLIVGGAFGIVNRTGAIEA
                     GIASVISKFKGKELLIIPISMILFGLGGTTFGMAEETLPFYMIFVPLMTSLGYDSLTA
                     IAIVFIGAAAGAAASTINPFSVGIAQALAQLAPGSGVTYRVVIFIAYILISIAFVMYY
                     AKKVKENPESSIVYEIDKNNKSILNQTSTEIKEFTFKEVSVLAIFGIGMAIMIWGVLS
                     QGWYIPEISMCFAAIGVLSGLVGRLSQIEISESFIDGAKDLASAALAIALARGIVIIA
                     QNGFIIDTILNSAAAFLGGLPKVIFVYLSFFLEAALAFLIPSSSGLASLTVPVLAPLG
                     DLVNISAQVIVTAYQFGVGLTNFITPTSGVLMGALAVAHIPFSKWVRFIMPILLILTA
                     VSLVFLTIGIYIGF"
     gene            22821..24188
                     /locus_tag="ACOIAF_001784"
     CDS             22821..24188
                     /locus_tag="ACOIAF_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HGZ2668797.1"
                     /translation="MGLKEFDFIEESIDMLRTMSPTLETISDEIEEYFENILDEKNQE
                     YINVTSRIKSESSLREKIIRNRYLKKYGEASNLIHNVSDLIGLRIECRFIEDENKIYR
                     LLRRYFNKTDDKINYYNKENKNIKLKLSERQPNKQKNGFEIYKIDGVFAYLDREVKFE
                     LQIKSLVNVFWSEIEHKIIYKNNTYLLADKFIKDMMDSIKNNLTMIDNQLLSIYKNFH
                     SGKSFNMKVSKKEIEKLFAKLVYDAFSEKMNKSIGFVVDFKKPCETILNYSFNKQDIS
                     DEVLGNFMLDEFARLNEIVNKDIDFNEQIEFEREPTFDDKFCKDLGNHFRSRLNTEFP
                     WNLFFRILFEIEPYNNTKDFENFVEFIKENVLLEENREQLLCQFEEDSKLIIEDIYDC
                     IFNSISEIDSVEILYNYNLEKINFTSSEIINCICREYECYGEYLEEKEKLMNTFKEKI
                     IQIFE"
     gene            24213..24818
                     /locus_tag="ACOIAF_001785"
     CDS             24213..24818
                     /locus_tag="ACOIAF_001785"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HGZ2668798.1"
                     /translation="MRHFSNIYELSKIILEEAICIEKTGVILAGGKNSRMGRDKAFLE
                     LHDKLFIEIAIEAFKNFDELIIISNNEELYSKYDIKVYNDIVKDVGPIGGIYTALEYA
                     KYDIVTIACDMPYLNNQIVERIANKMDDKSVISVTNGKLQPLCSGYKKSIINKVSLCI
                     RENDLKLRSFIDKIDKSYIYFDEDLFLNVNTVDEYEKLTKV"
     gene            24866..25237
                     /locus_tag="ACOIAF_001786"
     CDS             24866..25237
                     /locus_tag="ACOIAF_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HGZ2668799.1"
                     /translation="MNLLKCDSWAVILNNSDKESKAYKILDELKRNMYKVVAIDEEKK
                     PIEGIDVYECLKDVPHNIDVVAIIDKQSKMDVILEEVELLDIQNMWFEKGSFSEMMIK
                     KTKDLKLNIEYNLSLYDELTR"
     gene            25416..25754
                     /locus_tag="ACOIAF_001787"
     CDS             25416..25754
                     /locus_tag="ACOIAF_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HGZ2668800.1"
                     /translation="MLIVTTEKVEGKKISKVLGLVRGSTIRAKHVGKDIGASFKNLVG
                     GELTGYNEMLTEARQIAIGRMVEDAEAKGANAVIAFRLSSASVMQGAAEMLAYGTAVV
                     LEDDNGILEK"
     gene            26220..26723
                     /locus_tag="ACOIAF_001788"
     CDS             26220..26723
                     /locus_tag="ACOIAF_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HGZ2668801.1"
                     /translation="MFNVAIITLSDKGYEGKREDITGKKLTEFVENTGAYKVTEYVLI
                     KDDKEMLKENIIRLCNSNKIDLILTNGGTGFSKRDITPEATKEVLEKEIPGLSEYMRM
                     KSTEITKKAILSRGVSGIRNNSIIINLPGSPKGAVENLSFIIDVLDHGIEVLRGEATE
                     CATKKSE"
     gene            26770..27729
                     /gene="moaA"
                     /locus_tag="ACOIAF_001789"
     CDS             26770..27729
                     /gene="moaA"
                     /locus_tag="ACOIAF_001789"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HGZ2668802.1"
                     /translation="MIDKYGRKIDYLRISLTDKCNLRCVYCMPPDVKFDKNYINENLS
                     FEDYKFIIKAMAEQGITKIRFTGGEPLLYPKLNELIKFTKEECGINNIGMTTNAIGLS
                     DRIFELEKCGLNKVNISLDSLKEYKYKSVTRGGNLKDVLKSIESCLNLGIKVKLNCVA
                     ISGFNDDELKDFMFLTVKSPIDVRFIELMPLGEANRVYKKGYFNVKEMIEKIYGLYKV
                     KNDEKSTAEYYMFKGAKGRIGIITPLSCSFCNTCNRIRLTSSGAIKLCLHSKEEIDIK
                     PFLHKPLLFRESMKDIIKQKPEKHHLIENKCSDTNRRMYEIGG"
     gene            27733..28203
                     /gene="moaC"
                     /locus_tag="ACOIAF_001790"
     CDS             27733..28203
                     /gene="moaC"
                     /locus_tag="ACOIAF_001790"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HGZ2668803.1"
                     /translation="MLTHINEKGYAKMVDISEKDDTKRTAVATATITLSKEALEKVKN
                     GQIKKGDVLSVAQVAGIMGAKNTSSNIPMCHQIKLVGCDLEFDVDDSMSAIKIYATCK
                     SLGKTGVEMEALNACSLAALTIYDMCKAVDKKMVISNIHLVEKTGGKSGDFKFE"
     gene            28239..28670
                     /locus_tag="ACOIAF_001791"
     CDS             28239..28670
                     /locus_tag="ACOIAF_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HGZ2668804.1"
                     /translation="MAKVVSINISEKKGVIKVPVPCAELKINHGIVGDAHAGNWHRQI
                     SLLGKESIDKMKMKGFDQLKYGDFAENVTTEGIEVFSLPVGTKLRIGQCEVEVTQIGK
                     KCHNGCEIKKLTGDCVMPREGIFVKVLKEGIINVNDSIDVI"
BASE COUNT        10992 a         3170 c         4849 g         9682 t
ORIGIN      
        1 tttgaatata aaactgcaat atattaaaaa aattattcat tttaaacctt gtaatttgat
       61 tttataaata ttataattaa attaatgaat ttttaacaag gaattattca tttttttgat
      121 tttaagattt attgagaaaa caaaactaaa taggggggta attaatataa tggatttttt
      181 caatggtatt ttaacgagta ttaacaatgc agtagtatat gtaaacaatt acttatggtc
      241 gtatatctta attataatgt tagttgtaat tggtatttac tttactatta aaacaaattt
      301 tgtacaattc agatatttta tagaaatgtt tagacttctg ggtgatggta cagcgaataa
      361 aaatgcaaaa aaggaaggaa aaatctcttc atttcaagca ttttgtataa gtacatcttc
      421 aagagttgga actggaaata tagctggaat agcaattgca gtggtagcag gaggacctgg
      481 tgctgtattt tggatgtggc ttatagcatt aattggttct gcatcaagct ttgttgaaag
      541 tacattagct cagatttaca aagtgaaaga tggtcaagct tttagaggtg gaccagcgta
      601 ttatatggaa caaggtctaa ataaaaaatg gatgggtatt ttattctcaa tacttataac
      661 tatatgttat ggttttgtat ttaatgcagt tcaagcaaat actgtttcac ttgcatttaa
      721 taatgcattt ggaataagta aaatggctat gggaataggt cttggaattt taactgcaat
      781 agttattttt ggtggagtgc atagagttgc aaaagtttct gaaataatag ttcctgtatt
      841 tgcaggatta tatatattag ttgctttggc tatagttgta atgaatataa cagagatacc
      901 aagtgttata gcattgatag ttgaaagtgc atttgatttt aaaggaatgg cgattggtac
      961 atttatggga gttgtaatga ctggagtgaa gagaggtcta ttttctaatg aagcaggtat
     1021 gggtagtgct cctaatgctg ctgcaacagc acatgtaact catcctgtaa aacaaggttt
     1081 aattcaatct ttaggtgttt ttacagatac tataataata tgtagttgta cagcattcat
     1141 ggtattatta tattcaggtt atagtagtag tggagcaact ggaattgaat tagctcaaga
     1201 agctttaact catcatatag gaccagttgg aaatatattt atagcagttt gtatattctt
     1261 gtttgcattt agttctatag ttggtaatta ctattatggt gaatcaaata tggagtttat
     1321 gagtggaagt aagactaaac ttaatgtatt tagagttttt gttgtaggta tggttttatt
     1381 tggttcactt actcaagtag atgtagtttg gaatcttgca gacttattta tggcattgat
     1441 ggcgataata aacttaatag caatagcact tttaggaaaa tatgcattca ttgctttaaa
     1501 agattataca agtcaaaaaa aatcaggaat aaaagaccct gtgtttattg cagatgaaat
     1561 agaaggccta gaaaaggtat cagagtggca tagggaatta tctgaggaac atttaggata
     1621 aaaaatagta taaaattata aaaccatttt gaaagactag agtatgtata tactttatat
     1681 gaatataata ttgaattaat ttatattact ggaattttta attccagtaa tattttttta
     1741 attacaagga tattatgatg acttaaagac tgtggaaaac tcttttttag ttttcattat
     1801 taatattaat tgacaaattt agtttttatt taaatttctt aagtgcatat aaatcgtatt
     1861 ttttgataat cccattaagt ctgcaacttt aacaacagaa tccttaattt taaaaatacc
     1921 actttcattt aaaatagaaa caatagattt atttttatca ttgctagata tattagaatc
     1981 attttcaaca agtacctttg catcttggta tgattttaaa attacttcat cagtatcttc
     2041 tacgatattt tctaaaacag gagcactttg catagcatta tgttccggaa tatatgaacc
     2101 aattattctt gttagtggtg tattaagata aaaatgtatg cagagtagac cgattatttt
     2161 atcatcaact ccccttatca ctatatctgt tgattttaaa ggttcaccta gtttgtttct
     2221 attaaaataa ctgataaaat ctccatcagc atcattttcg agtttattaa tgagtgccaa
     2281 atcaaaatca gttataatat ctccttcttt tacagtatta tatttaccat tagctatttt
     2341 atttactgaa tagtgtactc cattaaattt atataaaata aactcgtatc cttctcctaa
     2401 atatgcagat aatccatcca ctagattgtt ataagaatcg aaaatatagt tatcagtcat
     2461 gttaaaagtc atatgttacc tcctaagaaa tatcttaata tcatattaac atataaaatg
     2521 aatttaataa aatatatttc agtgaaataa taaatagaat aaaaaaaaaa ctattgactg
     2581 aaaaatattt cagtgatatt attaaaatac agtaaggcaa acgtattact gacagaatat
     2641 ataaaatatt aaaaatagta tttaggaatt aaacaaatta aaggactagt gatttgaatt
     2701 tttttagtat aatatgtgac ttccaattaa tatttattaa acaccaataa aattgtaaat
     2761 atgcttaatg atatacttta gcgatgaagt gaaaatattt tatttttatg attaacctat
     2821 aggaattaat ttagcatact gtttagctag taaatctaaa taaattttag ctttagctga
     2881 tatcatgttg aataaagttt ctattggatt ttgtataaca aaggagtggg gagatggcac
     2941 aagataaaat tgtggttgaa attttagaaa gcattggtgg taaggaaaat attaaggaag
     3001 tagcacattg tatgactaga ctgagaataa aggtatgtga tagttctcag gtaaatcagg
     3061 aaaaactaaa gaaaataaaa ggtgtactag gtcttgtatt aaaaggtgag caatatcaaa
     3121 ttgttatagg tcctaatgta tcacaagtgt ataatactct taatgaagtt ataggaatta
     3181 tacctgagaa ggatagtaaa aaaacaatat ctgaaggtaa gcaaaatagt attgcaaaaa
     3241 tattagatac tattgcaggt atttttaatc ctatagttcc agcattagcg ggtgcaggtc
     3301 taataaaagc tatattagca ttactagtag catcaaagtt tttatctgca gatagtgaaa
     3361 cctatataat attaaataca atatctgatg gtgtattttc atttttacca tttatgttag
     3421 cttactcagc agctaaaata tttaaaatga atacttatgt tgctgtagca atagcaggag
     3481 caatgctaca tcctaatttc actaatttaa ttcaacaagg cttaactaaa atatctttta
     3541 tcggtattcc gatgtcacta atccagtata acggaagtgt atttccgata gtactaacta
     3601 tttggtttgc atcgtatata gaaaaaggag tagataaaat aacaccaaag gcacttaaga
     3661 taatattagt acctgcattg actattttat ttgcagctcc tatagcatta attattattg
     3721 gaccaatagc tcaaatgcta ggaaactata tagccggtgg agcaagtatc ctatttgaaa
     3781 gaggaggaat atttgcaggg ttaatttacg gaggaattta ttcattcatg gtagtaacag
     3841 gattacatca tggtatggtt cctgtattag tagatggaat aacaagatat ggatttaact
     3901 atataagtcc tgtgtcaggt agttcaaata tggctcaagc aggatctgca tttggagtat
     3961 ggttaaaagc aaaagataaa gatttgaagg ctgtagcagc tacagctacc atatcagcat
     4021 taactggagt taccgaacct gcaatatatg gtgttaattt aagattaaag aaaccgtttt
     4081 tatgcgctgc tataggtggt gcagcaggag gtggatttgc tgcatatttc caatgtaaag
     4141 catttgctat gggaggtcca tcgtttttaa ctcttcctat gtttatagga gaaggtggac
     4201 atgtaggatt ggtagcaata gcatttacaa tagcatttat agtatctgca atagctacct
     4261 atatagtagg atttgaagaa gaggtaacag cagattcaca cgctaactta taataaagag
     4321 aaattattta acatatcaaa aaggagatta attactatga aaatttattt aactaaagat
     4381 tatgaagaaa tgagccaagt ggcagcacaa atggtgttaa agtatatgta cagcaatgat
     4441 aataaaagaa taaacctttc tataacagca ggtcaaacac caaaaagaac atacgaaata
     4501 ttaagtgaat ttgttaagaa taaaccttat tttgaaaatg tacattatta taactttgat
     4561 gaaataccaa taaaaggcga aaaagtggga gttactataa gaaatttaac taatatgtac
     4621 tttagagaag ctcaaatacc agaagaaaga atacatgtgt tagatgaaaa taattatcaa
     4681 ggctttgata gaaagataga agccgatggt ggaatagaca tggtattaat gggattaggt
     4741 tgggatggac acttctgtgg aaatcttagt ggtacagtta actcttttga tgaagagaca
     4801 agagctgttt caacttatat aacagatgat atggctaaac tattaacaaa atctgtaggt
     4861 ggagatccag aaaaattatt tgattactat gtgacatatg gacctaaaac aataatgcat
     4921 tgtaaaaata taataatgat agtaaacgga aaacacaaag ctcaaatatt aaagcaaaca
     4981 ctatttggac cattagattt attatgtcca tcatctttat tgagattaca tcctaacttc
     5041 acattaatag tagatgaaga agcagcatct gaaataaaag atttaatata aataagtaag
     5101 gaattaaaat taaaaatgaa tttaggtgca aagtaatatt ttattttgca cctttgtttt
     5161 aataaaaagg agaataaata tgtataatca tatgaaaaaa gtattccaag gattagaggg
     5221 tgggttattt tcaagtgtag agaaagctga tgtaggcgat tcttatcaaa aattaggtga
     5281 acagggtgta agtttaatgg gatgggcaga tccatttttc ccagattact gtatacctaa
     5341 acatatatta gatgcagcta cattagcgat atcaagtaat atgtcaactc attatacagc
     5401 gcctataggt aatgatttat taaaaattga gatagcaaaa aaattaaaag aagtaaataa
     5461 tttagatgtt gattttaaaa gaaatatatt aataactccc ggatcagact cagggcttta
     5521 ttactcaatg ttaccgttta taaatgaagg ggatgaggtt cttatgccaa caccatgcta
     5581 tccaaataat atacaaaata ctaaattaat gggaggagtt cccatatttg tagagttaaa
     5641 ggaatcagat ggatatcaaa tagatataac agaatttgag aagagattaa ctaataagac
     5701 gaagttagta gttcttactc atcctaataa tccaacaact acaatattta ataaagaatc
     5761 tttagaaaaa ttagctaaat ttattataga caatgattta atattaatat gtgatcaagc
     5821 atttgaagat tttacttttg aaaatgaatt aataacacca gcttcattag aaggtatgtt
     5881 tgaaagaaca gtaacaatat tttcaacatc aaaaggtatg ggattaagtg gatttagagt
     5941 tgcatatatg gtatgcagcg ataaattaat ggacaaatac tatggagcag cagtgacagt
     6001 attaggtgct actaatacag tagctcaaat gtcaacgatt gaagcattta aagatagaag
     6061 ctttatggag gattttgaaa gagtttttga taaaagaagg caagaagcat ataagataat
     6121 caatgaaata cctaatgtat ctatgcagat gcctgagtcc ggatttttag gttggataaa
     6181 tgtatcaaag cttggaacat catcagaaat acaaaagtat ctagttgacg aggctaaagt
     6241 ttgtattaat gatggaatta attatggtcc aggaggagaa gggcatttga gaataatttt
     6301 aggaagctat aaagacgata gtaaggtaat tggtgcatta aatagaataa aagatgcttt
     6361 aattaaaaag caacaataaa taaaaaataa ttggagtgga gtatgttatc aatatttaag
     6421 agtaaaaaaa atgacaacaa gcttaaggca tttgcatcag ggaaagtaat tcctatagaa
     6481 gatgttgatg ataatgtttt ttcatctaaa atgttaggtg atggaatcgc tatttatcca
     6541 actaatgaat atgttgtagc cccttgtgat gcagaagtaa atataataat gaaagatact
     6601 aaacatgcag ttggaattaa attatttaat ggcatggaaa tcttatttca tgttggaata
     6661 gatactgtat ctatgaatgg agaaggtttt gagatatttg tttcaccagg tgacaggata
     6721 aaaagaggtg ataaattaat atcttttaat agtaaattaa taaaagaaga agggttacaa
     6781 gatgtagtta taatggtagt tactaattca gaacagtata aaaatataaa atatcatatt
     6841 ggactggagg cagtaatcga tgatactatt attgcagaaa tttaaaaata gttatttaag
     6901 taaatactta gataatttag atatagttaa aattaaatcc ttaaaatcaa attatttaat
     6961 ttttgtgtaa ttttaactat aaagccgatt aataatataa agtatactaa ataatatggt
     7021 tattatgatt gttactgaca aagcctaagt agatataaca atttgctcta attgagtatt
     7081 gctatatcta cttgttttta tatttaaaat aaaagagtct gcattgaaaa gataattctg
     7141 atgagagtaa atagggtatt taataatagt ttaattctga cttatcaaga tgaacctatt
     7201 catataccta aaacatcttt aacaccttct aaattcatac aaatagctgt ataggctgcc
     7261 ttattgatta tttttccgtc aatttttact ttgtagtgaa tagcatccat aaatattatc
     7321 gaatagattg tatcaagagc tctattttgc tattcattag ctaaagatag tactttgtca
     7381 gttatttttg aaactaatgc atgagatata tcaattccgt aaatattatt taaatgatat
     7441 gatatatatc gcgagtcgac attccttttg agtacatgcc aattacttgg ttctctattc
     7501 cagaaatttc attttcgtaa ttttgaataa ccctaggctt gaaatcatcc ctagggatat
     7561 ttaattttac ctctccaaaa tctgatttaa cattcttagc tctatatcca tttcttgaat
     7621 ttgatgttgc tttactttgg ttatcatatc tctcgtacat taaatgatct tcaatctctg
     7681 actctaacgt tttgtgaatt gtgcttgcta ataaatcttt taacatctct tggatgtctt
     7741 tagtattttt tacatcatac tcttgaacta agtgttttaa tatttaattt ttgttgtaca
     7801 taaaaaatgt gtacctccta ttttggtaat tagatttaat atcttgactt ttaggagaat
     7861 acacatttta ttttacagac cctcttttct agaaaagttt ttatgaaaat tgtgatataa
     7921 attattaata aaaaataatt ttatatttat tttattatgt aatttagttt tattaaaaat
     7981 tttttattat tggatataga ttatctatta caaattttat ctttaagttt attctcctaa
     8041 aatactatat aattaaatcc tgtaaattat cttcaaagct ttctaggtaa tcacaaaata
     8101 atctaaattc ttctatggtt ttaatttctt gtaaatttct caaatctatc attatattac
     8161 tatatttatg aaacataaaa ttcatatcat acatttcaca tttaacaaat tcattttgat
     8221 aactatattt ataaggttcg tattttgaaa aattcgactc aaaatatttt attttagcat
     8281 aacataattt gtgtatgttg aacaaaagtg caagaacaga ctccttaact gcaaacttat
     8341 gtgtaaagta ttttatcgtg tggtatttac catgtttaaa ctcccaaatt aaatcctcat
     8401 ttaaagtaag agaatatttt tttatgaggt tacgcgtatc tagtgttaaa ttttgtattg
     8461 aatgtatttt cataatttat tctcctcata aatatttaat aagtattaaa ttatagtgtt
     8521 ttattattaa ttgctcttaa ttatattgta tggtaaatta aaaaagtaat aaaatagata
     8581 attaatatac tagtaatata tgctataaat aaaaacaata gctaaaatag taatatatat
     8641 gtttttatga tattttatgt ttagggttat atttgtaatt attttaagct tctaaaagta
     8701 gatttgatgg ataacgtata attttagaag gatatgcaat ttgtttgata agaacgtata
     8761 tatgattaaa ttaaaaatat taagtaccaa aaagaagaaa gatagtttct ccctttggta
     8821 cttaatattt tgtatccttt acactaattt ttaatattta ttaatgatat tatttaatct
     8881 agagacttaa ataaatttgc catttctagc gcattcatag cacaatcata acctttattt
     8941 ccagcttttg tacctgctct ttcaatagct tgttcaatat tttctgtagt tacgattcca
     9001 aatataacag gtaattcact gtctaaagat actttggcta tccctttaga gacttcacta
     9061 catacataat cataatgaga agtagcccct cttataacac agcctaaaca gattatagca
     9121 tcatatttac cacttttagc cattttttga gcaacaagag gaatctcaaa agctccaggt
     9181 acccaaacta tttctatatt ttctggagaa acgtcatgtc ttaaaagaca gtcttcagct
     9241 cctgataaaa gttttgatgt tataaattca ttaaaacgag aatttattat acctattttt
     9301 aaatcttttc caataagttt tccttcatat atcattttta tttcctccaa ttttatttat
     9361 gttatagatt tatatttata aatatcttta atttaattat aaaaaactac ttagtagaaa
     9421 aatatcatca tataatcatt tatataatat ctaaaagatg tcccatttta tcttttttag
     9481 tttttaaata tagcttatta aagtcattaa tttcttcatt aatagggatt ctattttcga
     9541 tttttattcc atatttctct aattgattta ttttatctgg gttattactt aaaagattta
     9601 tactttttat gtttaaattt tttaatattt gagcagccat atagaaatct ctcatatctt
     9661 cttcaaatcc aagcattaaa tttgcttcaa cagtatcgta accttcttct tgaagtttat
     9721 aagccttaat tttatttaaa agaccaatac ctctaccttc ttgcttcata taaattaaaa
     9781 ctccacttcc attttccaca attttcttca tagaagattc tagctgtaat ccacaatcac
     9841 attttagaga gtggaacaca tctcctgtta aacattcaga atgaagtcta actagagtat
     9901 tttctatatt tatatttcca taagtaaggg caatgtgttc ttcatttgaa taagtatctc
     9961 tatatcctat gatttcaaaa tttccatatt ttgtcggaag gaaagcgcta gatacttttt
    10021 ctatagtagt ttcatttatt ttgcggtatt ctataaggtc ttttatagtg atgattttta
    10081 aattatgctt ttctttgaaa atcattaaat catcaactct tgccattgta ccatcatctt
    10141 tcattatttc acagataaga ccaatctctt taaaacctgc aagtctagca aggtctactg
    10201 aggcttctgt atgtccatcc ctgacaagaa ctccaccagt ttttgctatt aaaggaaaga
    10261 tgtgaccagg cgttttgaaa tctttacttg tagaattttc atcacagaat aattttacag
    10321 tttcagctct ttcatatgca gatataccag ttagagtatc tgctccatct atagtaacag
    10381 taaaggctgt ttgaaatgta tctgtatttt ctctaaccat aagaggtata ttaagacttt
    10441 taaatttttt ttcagtggca ggtagacaaa ttagacctct accatatgta gccataaaat
    10501 ttattgattc aggagtagcc atttcagctg ccatcaataa atctccttca ttctctctac
    10561 tttcatcatc aactacgatt accattttcc cattttttat atcttcaata gcttcttcaa
    10621 tagtattaaa catgtaattc actcctttat tgtttttaaa aaccattttt aaataaaaaa
    10681 tctttatcta tatttgattt atggttacta ctattagaat ttaattcctt gtagttatta
    10741 gccataaaat tattaacata ctttccaatt acatcgcttt ctaaattaac gaaatctcca
    10801 acattctttg tcaagagaat agtgttaatt ttagtatgag gtataataga aaccttaaat
    10861 atttcatcat ctaaatatga tatagttaaa cttacaccat caatagcaac agaacctttg
    10921 agtatcattg aggataaaag ttttttatca ggtcgtatac ttactaaaac tgcattttca
    10981 tctttttcaa atttgcagat agttccttta ccatcaacat gacctgttac tatatgacca
    11041 ccaaatcttg aagaaagact catagctctt tcaagattta ccttgtcatt tgcatttaat
    11101 aaacctaaat ttgtggaacg aattgtctcc atcataacat cggctgtaaa ttcatttttt
    11161 ccgaggtttg aaacagttag acaaactcca tttacggcta tactatctcc aagatttgtg
    11221 ccatctaata ttttatttga tagtatggtt acttttccac tcttaccatt agcagtaatt
    11281 tttctaagta ttccaacttc ttctactatg ccagtaaaca tcctaacctc ctatatgaat
    11341 gtattaacaa aaatttaatt taaaacatct gattctataa gaatatcgcc atctatcaaa
    11401 gttacattat tgattgttaa ctgtgttgaa tctgacaaat gatttattcc ttctccagaa
    11461 acgaatgtag gtgcatcttt accacctata atcttgggtg atagaaataa tttgacttta
    11521 tcaactaact tatttctaaa gagactgtca ttgagagtag caccaccttc aactagaaca
    11581 gaatcaatgt tcaactctcc taatttatac attacaaact ccaaatctac taagttgttt
    11641 tttgaaggag aaattattgt ttcaacatta ttttctttta acatagataa ttttatactt
    11701 tctttgcaac atgtgaaaac tatagtttta ctggttttat ctttaaccaa attactatta
    11761 agtggcaact ttaggtgtgt atctattact attcttgtag gatgagacac tttttcttga
    11821 ttgagcctgc aagttagtaa aggattatcg ttcaaaactg tatttatgcc aaccattata
    11881 ctttggtatt tatttctgta tttatgagtt agataccttg atgattcatt agatatccat
    11941 tttgattcta gtgattttgt agcaatttta ccatctagac ttactgctga ttttacaata
    12001 caaagaggtc ttttgttttt tatatgataa aaaaatgcct catttaaatc tcttgcttca
    12061 tcttctaata ttcccacagt aacatcaata ttgttatctt ttaatttttt tactccatta
    12121 ccacaaacaa gtggatttgg gtctaatgta gatattacaa ctctcttaat cttattttgt
    12181 ataattttat ctacacaagg aggcgttttc ccataatgag aacatggttc taagtttaca
    12241 taaagagtag agtcttttag actttgttta gcactgttta tagcatttac ttcagcatga
    12301 ttagagccaa atttctcatg atatcctttg cctattatat tactatcttt aacaattaca
    12361 catccaacta atggatttgg atttacaaag ccttctccat ttttagccaa ttcaatagct
    12421 tttttcatat aataaatatc tttttctttt tgattcacaa aattcacaat atgtacacct
    12481 cctgtttaat tggataattt ttaaacaaaa aaccctgaga attaactcag ggtttagaat
    12541 aatctacaat aaatgtttaa taaatgtaga acaaaattgc tctacaaaaa cactcattaa
    12601 atatcttctt ccatccagac tttactgtcg gctttggaat ttaaccaaat caaccaagtt
    12661 ttacattggc tcgcgggcta taccgccgat tgggaatttc accctacccc gaagattaag
    12721 tattaaattt atatttagaa tgtatcataa attattttac tttgcaaact atttttttga
    12781 aaaatatatg ttaatatata atatagaatg tctataaact ttgaaatacc acgattgtga
    12841 atataaaaat aaaaatttga acataatcag gaaagaagtc taattaattt aaattaggag
    12901 agtaatatat gaataaaaaa cttttagaaa tactatcaaa atactatgga tatacaagtt
    12961 ttagaaaagg tcaggaatct ataataaatt ctatcttaag cgaaaaagat gtgctagcta
    13021 ttatgccaac aggaggggga aaatcaatat gttatcaaat tccagctttg attttagatg
    13081 gaatgacaat agtaatttct ccacttatat cacttatgaa agaccaagta gatgccttaa
    13141 agactatggg aattagtgct gcttttataa atagttcatt aagtagcaaa gaatttaatg
    13201 aaatattaaa taatattaga aaaaataatt ataaaatact ttatattgct ccagagagat
    13261 tagatgcaca agagtttatt gagctaataa ataataacaa tgtaagtcaa gttgcgatag
    13321 atgaagcaca ttgtgtatct caatgggggc atgattttag gttgagttat agaaggattt
    13381 ctgattttat aaaaaatttg cctaaaagac ctatagttac agcatttact gcaacagctt
    13441 cagaagaggt aagaactgat ataataaatt tactgtgttt agaaaatcca gattattata
    13501 taactggatt tgatagagag aatttatcaa taaatatagt aaaatcatct agtaaaaata
    13561 agtatatttt agattatatt caaaatcata aaaatgagtc tggaattata tatgttgcaa
    13621 ctagaaagga agttgaaaat atatctaatg gtttatctaa gagaaatata tctgtgtcaa
    13681 aatatcatgc aggtttatct aaaaatgaaa gaagtaaaaa tcaagaagat tttataaatg
    13741 ataatgtgga tataatggta gcaacaaatg catttggaat gggtattgat aaacctaata
    13801 taagatgggt tatacattac aatatgcctc aaagtataga aaactactat caagaaatag
    13861 gtagagctgg tagggatgga gaaaaaagtg agtgtgtact tttattttct ccaggagatg
    13921 tacatataca aaaatattta attgatattg gagttgaaaa tcctgaaaga aaattatttc
    13981 agcataagaa gcttcaatac atgattgact tagtttacag taattcatgc tatagaaaga
    14041 caatattgag ttattttgga gaaaattata tagacaattg caataattgt agcaattgtt
    14101 tagtagaggg tgaaatagta gataaaacta tagatgcaca aaaagtcatt tcatgtgtag
    14161 ctagaatgaa aagaagttat ggtgttacta ctattgtaga tgtgcttaga ggttctaaga
    14221 ataaaaagat tttacagctt ggattaaata ctttgtctac atataacatt atgagagatt
    14281 attctagtga agacttaaag aattttgtaa atacattggt ttctcatggt tttttagatt
    14341 tagttgagac tctaggcaat ggaaatagag gtagttttcc tactataaga ttaaatgaga
    14401 tgtctatgaa agttttaaaa ggtgaagtga aagtagagtt taaagaaatt gttatgacaa
    14461 aatcattgga agtagaggat gagttatata atgctttaag agaacttaga cattcaattg
    14521 caagtgaaga aaggatagct ccatatatgg tatttggaga tggtacactt agagccatgt
    14581 ctagtagtta tccaattaat aaagaagaaa tgctagatat ctctggagta ggagaaatta
    14641 aatatcaaaa atatggtaga gattttgaag cagtaattaa agaatatgtc gaaaaaaata
    14701 atataaataa aaaaatagat gaaaaaaatg tcgattcaaa gaacgttaat agtgatttct
    14761 tagaggttaa tacagataaa gttttatatg aaaaactgtt agtcattaga gaagaatatg
    14821 caacaaagga aaaattacct gcatatatga ttttaaccaa taaggctttg aaagaaataa
    14881 gtggaagata tccacttgat gaagaacaat taaaagatat ttctggcata ggtggagtaa
    14941 aatttgaaaa atatggagct agtattttgg cagaggttaa taaatatgta aaagaaaata
    15001 atattaatgt tatttgggag aaaaagggca aaaggaagtt aatattagat ggagaaagta
    15061 gaaaaaataa tgaaatagca ttagatttgc taaatcaagg aaaagatata caaagtatca
    15121 gtcaagaaat agaagtttca ctgtcaacca ttattggata tgtgcatgac tatgtaaagg
    15181 atggacatag tatatcattt gaattaggac tagaaaattt ttataatgaa gataaaaaga
    15241 agatgatttt agaagtttgt gaaaaagttg gctatgataa tttaaataac ataaaaagaa
    15301 aacttccaga ttcaataaag tatgaaaata taagagctgt gatacttgac tcttatcttg
    15361 aaaacttaaa aaataatatt atagcagagt aatttttaac ttaataaaaa ataaaatttg
    15421 tattattgtg ttgaactaat aattttgctg tgttatactg aatttgtaaa aataatgaaa
    15481 aattagagct aggggcgcta gaattggcta gctgagagat aaagccgtaa cttacaaacc
    15541 ctaataacct gatcaagata atgcttgcga agggaagctt tttaatataa ttttattata
    15601 taaataggca aattattcaa tgttaggaga aatttgtcta agtgtatata cgattataag
    15661 agtgctttcc aaacgaggaa aagcactttt tttattttca aaaactataa ctttaatctt
    15721 ggaggattgt aatatgaact atacaacaca aatggatgct gctagaaaag gcataataac
    15781 taaagaaatg gaaatcgttt ctcaaaaaga acaggtagat gtaaatgaat taagagaatt
    15841 aatagcaaat ggacaagttg ttataccagc taataaaaat cataaatctt taagtgcaga
    15901 aggtgtaggt aaaaatttaa gaacaaaaat aaatgtaaat ctaggaattt caagagattg
    15961 taaggatata gaaaaagaac ttgaaaaagt tagagtagca atagacatga aagctgaagc
    16021 tataatggat ttaagtaatt atggcaaaac tagagaattt agagaaaagg ttgttgaaat
    16081 gtcacctgca atgataggtt cagtacctat gtatgatgca gtaggatatt tggaaaaaga
    16141 attaaaagat ataacagaag aagagttttt aaatgtaata agacaacatg ctattgatgg
    16201 agtagatttt attactatac atgcaggtct tacaagaagt gtttgccaaa aaataaaaaa
    16261 tcatgagaga ttgactcata tagtatctcg tggaggttct ttattgtttg catggatgga
    16321 attaaataat aaggaaaatc ctatttatac taattttgat aaaatattgg atatatgtga
    16381 agaatatgat gttacattga gtttaggaga tgcttgtaga ccaggttgta taaaagattc
    16441 tactgatggt gttcaaattc aagaacttgt agtattaggt gaacttacta aaagagcatg
    16501 ggaaagaaat gtacaagtaa tgatagaagg tcctggacac atggctattg atgaaataga
    16561 agcaaatgta gtattagaaa agagattatg ccatggagca cctttttatg ttcttggacc
    16621 attggttaca gatatagcac caggttatga ccatataaca agtgcaatag gaggagcttt
    16681 agcatgtgct aaaggagtag attttttatg ttatgtaact ccagcagaac atttaagatt
    16741 gcctaattta gatgatatga aagagggtat aatagctgca aagatagctg ctcatgcagg
    16801 agatatagct aaaaatgtta aaggtgctcg tgaatgggat aataagatga gtaaagctag
    16861 agctgattta gactggtgtg agatgtttag acttgcaata gaccctgaaa aagctaagag
    16921 atacagagat gaatcaactc ctactcatga ggatagttgt actatgtgtg gaaaaatgtg
    16981 ctcaatgaga acagttaaga aaatattaaa caatgaggaa cttaatctaa tataattttt
    17041 ggctagtatg actggaataa aaataagctg aaaagaggag gtgatttagt gaaggttaat
    17101 gggaaggaaa ttgaatttga aaaagatttg actgttatag atttattaaa taaatataat
    17161 ttaaaaagtg atagagttgt agttgaagtg aatttagaga taattgaaga atctaactac
    17221 aatacatatg tattaaaaga cgaggacatt gtagagctta ttaactttat tggaggtggt
    17281 taaaataaaa atatttgtaa atgaattatt aacagatgtt gaggaaaata caactgctta
    17341 taaagttaga gatagctata acaataaatg tgatgtattg gtgttaaatg gatatccaat
    17401 taaaacagat atgccccttt gtgaaaatga taaattaaca cttatacaaa aaggagttaa
    17461 gccatcactt gatgaattgg aaagcttgtt gattgctaga catactccaa atatacataa
    17521 taaattaaaa aaagggaaag ttgctatatt gggattgggg ggacttggtt ccaatatagc
    17581 tatctcttta gctagaatag gagtgggaga acttttactt atagattatg atgttgtaga
    17641 accatctaat ttaaatagac agcagtattt tatagatgat ataggaaaac taaaaactga
    17701 tgccatgata gaaaatataa aaaaaataaa tccatttata aaattaaata aacgagatat
    17761 ttttttaaac aagaacaata tggatactat taaggattca gatttaataa ttgaggcctt
    17821 tgatgatgct tcatgtaaag cacaggtatg taattatgta ttaataaatt taaaagataa
    17881 atatttaata gcatcatctg gtatggcagg ttactatgat tcaaatataa taactactaa
    17941 gaagataaaa gacaggtttt atatatgtgg ggattttgtg aatgaagcta aatttggtga
    18001 agggcttatg gctccaaggg ttgccatctg tgcaaatcat atggcaaatt tggcaacgca
    18061 aatacttata gatatgtaaa tattaaaatt agatataaaa aagattgaat attaaaaaaa
    18121 tttaacgtgg agggaaataa tatggataaa ttggtacttg gtggacatga atttaatagc
    18181 agattattgg ttggaacagg taaatatggt tcaaataata tattgcctga ggtaataaaa
    18241 gaaagtggaa gtgaaataat aacaatggca cttagaagag tggatttaga caataaacaa
    18301 gaaaacatat taacgtatat tccaaaagag atgacaatac ttccaaatac atcaggagct
    18361 actaatgcag aagaagctgt aagaatagct agaatatcta gaaaaatggg atgtggagat
    18421 tttataaaaa tagaagtaat atcagatact agatatttac ttcctgataa tgaagaaact
    18481 ataaaagcta caaagatact tgctgatgag gggtttatag ttcttccata tatgacacca
    18541 gacctttatg ctggaagaag acttatagaa gctaatgcag cggcagttat gccacttgga
    18601 gctccaattg gttcaaatag aggtcttcaa atgaaagaaa tgataagaat aatgatagat
    18661 gagttggata taccaataat agtagatgca ggtattggaa aaccatctca agctatggag
    18721 gctatggaaa tgggagctga tgcagtactt gtcaacactg ctatagcttc agcaggagac
    18781 cctgtacaaa tggctagagc atttaaatta gctgtagaag gtggaaggga agcatatatt
    18841 gcaaaaacag gaaatgtgtc agaatttgca aatgcatctt ctcctttaac aggtttttta
    18901 ggcaatttat agaaagtggt gattggtgat gagtttttat gatgtaatag aaaaatatag
    18961 ggattttgat tttgatggat atttaaataa tgttactgat aatgatgttt taagaagttt
    19021 atcaaaagac aagcttgaag attttgatat attaaatttg ctttctaaaa ctgcagtaaa
    19081 acatttagaa gatatggcac aaaaagcaca taaattaagt gttcaatact ttggtaaaac
    19141 agtatgttta tatacaccaa tgtatatagc aaattattgt gttaatcaat gtgtttattg
    19201 tagctataat ataaagagtg gaataaaaag aaaaaaatta actatggatg aaattagaga
    19261 agagggagaa gcaatttcaa aagaaggttt caaacatcta cttgttttga ctggagaaag
    19321 tagttttcac tcaagtgtag aatatatagg tgaggctata gaaatattaa gagagaaatt
    19381 tccctctata ggtatagaag tttatccaat ggaagtagaa gaatataagt acattgtaga
    19441 taaaggtgtt gaaggattaa cagtatatca agaaacttat gatgaggaaa tatataagag
    19501 agtacatata aaaggcccaa aatcaaatta taaatttaga ctagatgctc ctgaaagagg
    19561 ggcaaaagca ggtatgagga ctttatcaat aggtgcactt ttaggactaa atgattttag
    19621 aaaagaaact ttttttacaa tattgcatgg gaaatactta aaaacgaaat atcctcatat
    19681 tgagctttca tattcaacac caagaatgag accttttaaa gggtgttttg aggaattggt
    19741 agatataagt gatactgatt tagttcaagc tatggtatgt atgagattat ttgaccctca
    19801 tgcagctata aatatatcca ctagagaaaa tttagaaatg agaagtcata taataccttt
    19861 aggagtaact aaattgagtg caggtgtatc tactgatgta ggaggtcatt ctcaagatga
    19921 acatgatact gctcaattta agataaatga tgaaagtact gttaaagatg tggagaaaat
    19981 gctaaactca attggttatc agcatgtgtt taaagattgg gaaagatttt aaatgtatct
    20041 gataacaaac agaaaattat gtagtgagga acgatattta gaagttatta aagagtctat
    20101 attgagtggt gtagaaaata taataataag agaaaaggat ttagaatatc aagagctaag
    20161 aaaattatat atgaaaataa aaactaaaat caattgtata gattttcaag agcaaatatc
    20221 agatgaaagt ttgaaaacta atataaatca aaaagagtgt agaaataaat tcaaggttaa
    20281 ttttataata aatagtaaca ttgaattttt tgaaaaagtg gattgtcaag gtatccatct
    20341 tccttttaaa ttattcttga atttaattga aaataagtat aattttaatg aaaataaaat
    20401 actaggatta tctttacata aagtagagga agtagattat ttagaaaaat taataagaaa
    20461 tcaaaatata aaaatagatt acataacatt atcacatata tatgaaacta aatgtaaaga
    20521 aggattaaat ccaaaaggaa ttgagctttt aaaagaagct aaaaagatta ctgatattaa
    20581 aataattgca cttgggggaa ttttaccatc taatgtcaaa gaaacattaa aatactgtga
    20641 tgattttgct ataatgtcaa ctataatgag gtctaaagat attaaaaaaa ccatatcaaa
    20701 ttataatgaa aagttgaatt agtaaatata gtatattgaa tttttaactt aaataaaaat
    20761 aaaaaagtta tcttttaaat tttaaaaggt agctttttta ttttatatct ttgctaataa
    20821 aaaattgtaa aaagggaaaa ctttagtcag agagattaaa caatatccta gaaagattta
    20881 aaatttagta aagtatggag gacattataa tgactgacag taaaaagaaa cgatttatta
    20941 taccacacac ttttacaata atatttttac taatagtctt gatggcaata ttgacttgga
    21001 tagtaccaag tggtgaattt agtagagaag atgtagaagg tagaatggta gttgtaccag
    21061 ggacttatca aagtgtagaa agtaatcctc aaggatttgc agatgttttt aaagcaccaa
    21121 tagaaggctt tatcgattca gctgaagttg taggttttgt tttaatagtt ggaggtgctt
    21181 ttggtattgt aaataggact ggagctatag aagcaggaat agcttcggtt atctctaagt
    21241 ttaagggaaa agaacttctt ataattccaa tatcaatgat tttatttgga cttggaggta
    21301 caacatttgg gatggcagaa gagacattac cattttatat gatatttgtt ccactcatga
    21361 catccttggg atatgattct ctaacagcaa tagcaattgt atttatagga gcagcagcag
    21421 gagcagccgc ttcaacaata aatccatttt cagttggtat tgcacaggca ctagctcaac
    21481 ttgctcctgg ttcaggagta acttacagag tagttatttt tatagcatat atactaataa
    21541 gtatagcatt tgttatgtac tatgctaaga aggtaaagga aaatcctgaa agttcaatag
    21601 tatatgaaat agataaaaat aataaatcaa tacttaatca aacttcaaca gaaataaaag
    21661 aatttacttt taaagaagtg tctgttcttg ctatatttgg aatagggatg gcaataatga
    21721 tttggggtgt tttaagtcaa ggatggtaca ttccagaaat atcaatgtgt tttgctgcaa
    21781 taggtgtgct ttctggattg gttggtagat tatctcaaat tgaaatctca gaaagtttta
    21841 tagatggagc aaaagattta gcatctgcag ctcttgctat agctttggct cgtggaatcg
    21901 taataattgc tcaaaatgga tttataatag atactatatt aaattccgca gcagcctttc
    21961 taggtgggct tccaaaggtt atatttgtat atttatcatt tttcttggaa gctgcattgg
    22021 catttttaat tccatcatct tcaggtcttg cttcacttac agtacctgta cttgctcctc
    22081 ttggtgattt ggttaatata tcggctcaag tgatagtaac cgcttatcaa tttggtgtgg
    22141 gtcttacaaa ctttataact ccaacttcag gagtactgat gggagcactt gcagtagccc
    22201 atattccatt ttcaaaatgg gtaagattta taatgcctat cttgttgatt ttgacagctg
    22261 tatctctagt ctttttaaca ataggtattt atatagggtt ttaattaagt gtagttttat
    22321 ttaagatatt tagtaatggt ataaaatgat aatatgtttt caataaattg ccactaattt
    22381 aattagtggt aatttgtttt ttgtatttaa aaataaatgt atttacttgt attttgaatt
    22441 aatatctact tagacattgt tttaattgtt atattttcat ttaattaagc attgatttat
    22501 cattttttat atttcatata acaatgcaaa aaataatttt tatatagtaa atgacaaaag
    22561 tttacaataa atgtatattt tcttactcat tccttaaaat tactcattta gtttaataac
    22621 tctcatttca tatgtaaagt tggcaaaaaa attaaaatta aaagtaaaaa actaaataaa
    22681 ttaatgttaa tttgaaatat atatgaaggt ttttggagaa tacttactta tgataggaaa
    22741 aaatgttaaa ataatagtgt aatcaaatat aaaggactga aataattaac agttattaaa
    22801 tattaggagt gtaaaaattt atgggattaa aagaatttga ttttattgaa gagtctattg
    22861 atatgttgag gacaatgtca ccaacattag aaactatatc agatgaaata gaagagtatt
    22921 ttgaaaatat cttggatgaa aagaatcaag aatatataaa tgtaacctct agaataaaat
    22981 cagaatcaag tttaagagaa aagataatta gaaatagata tttaaaaaaa tatggagaag
    23041 caagtaatct tattcataat gtttctgact taataggcct aagaattgag tgtagattta
    23101 ttgaggatga aaacaaaata tatagacttt tgagaagata ttttaataaa acagatgaca
    23161 aaataaacta ttataataaa gagaataaga atattaaatt aaaactatct gagaggcaac
    23221 ccaataagca aaaaaatgga tttgaaatat ataaaataga tggagttttt gcgtatttag
    23281 atagagaagt aaaatttgaa cttcaaatta aatctcttgt aaatgtgttt tggagtgaaa
    23341 tagaacataa aataatttat aaaaacaata catatttact tgctgataaa tttataaagg
    23401 atatgatgga ctctataaag aataatctta ctatgataga taatcagctt ttaagtattt
    23461 ataaaaactt tcattcaggg aagtctttta atatgaaagt aagtaaaaaa gaaattgaaa
    23521 aattatttgc gaaattagtc tatgacgctt tttctgaaaa gatgaataaa agtataggtt
    23581 ttgttgtaga ttttaaaaaa ccatgtgaga ctatattgaa ctattctttt aataaacaag
    23641 atataagtga tgaagtttta ggtaatttta tgcttgatga gtttgcaaga ttaaatgaaa
    23701 ttgtaaacaa ggatatagat tttaatgagc aaatagaatt tgaaagagaa ccaacatttg
    23761 atgataagtt ttgtaaagac ctaggaaatc attttagaag tagattaaac acagaatttc
    23821 catggaacct attttttaga atattatttg aaatagagcc atataacaat accaaagact
    23881 ttgaaaactt tgtagagttt ataaaagaaa atgtgctact agaagaaaat agggagcaat
    23941 tattatgcca atttgaagaa gatagcaagc ttataattga agatatatat gattgtatat
    24001 ttaatagtat aagtgagatt gatagtgttg aaattttata taactataat cttgaaaaaa
    24061 taaactttac atcaagtgaa atcataaatt gtatatgtag agaatatgag tgttatggtg
    24121 aatacttaga agaaaaagaa aagcttatga atacatttaa agaaaagatt attcaaatct
    24181 ttgagtaata ggtcaatgct gaagtggcgc taatgcgcca tttttcaaat atatatgaat
    24241 taagtaaaat tattttggag gaggcgattt gtatagagaa aacaggtgta attttggcag
    24301 ggggaaaaaa ttctagaatg ggtagagata aagccttttt agagttacat gataagcttt
    24361 ttattgaaat tgcaatagaa gcatttaaaa actttgatga gttgataata atatctaaca
    24421 atgaagaatt atattctaaa tacgatatta aagtttataa tgatatagta aaagatgttg
    24481 gtccaattgg aggaatatat actgctcttg aatatgcaaa gtatgatata gttacaattg
    24541 catgtgatat gccatatcta aataatcaaa tagtcgaaag aatagctaat aaaatggatg
    24601 ataaaagtgt gatttcagtg acaaatggaa agttacaacc attgtgttct ggatataaaa
    24661 agagtataat aaataaagta tctttatgta taagggaaaa tgatttaaag ctcagaagct
    24721 ttatagataa aatagataaa agttatatat actttgatga agatttattt ttaaatgtaa
    24781 acactgttga tgagtatgaa aaattaacta aagtttaatt agctatggtt gtaaaataga
    24841 gtaggattta tatggaggag agtttatgaa tttacttaaa tgcgattctt gggcagtaat
    24901 attaaataat agcgataaag aaagtaaagc atataaaata ttagatgaac ttaaaaggaa
    24961 tatgtacaaa gttgttgcta tagatgaaga gaaaaaacct attgaaggaa tagatgtgta
    25021 tgaatgttta aaagatgttc cacacaatat tgatgttgta gctattattg ataaacaatc
    25081 taagatggat gtgattttag aagaagtaga gcttttggat atacaaaata tgtggtttga
    25141 aaaaggaagc tttagtgaaa tgatgattaa aaagactaaa gatttaaaat taaatataga
    25201 atataattta agtttatatg atgagttgac tagataaaat tatatttctt aataataaaa
    25261 atataattag ttaataaaac atattatttt atagtataga ttggttaata aaagtacaga
    25321 ttggttaatg aaacattaaa ttacaatatt tgttaaaaaa attgtgagtt acatgatata
    25381 atgtattaaa taggatacta aggagttgat atattatgtt aatagtaact acagaaaaag
    25441 tagaaggtaa aaagatatca aaggttttag gattagtgag aggaagtaca ataagagcaa
    25501 aacatgttgg aaaagatata ggagcaagtt ttaagaatct tgttggagga gaacttactg
    25561 gatataatga aatgctcact gaagcaagac aaattgccat aggtagaatg gttgaagatg
    25621 cagaagctaa aggtgcaaac gcagtaatag catttagact gtcttcagct tcagttatgc
    25681 aaggggcagc agaaatgctt gcttatggaa cagcagttgt tttagaagat gataatggta
    25741 ttcttgaaaa ataaaatata aatagttaaa aagaggcatc ttttaaaaat tttatggatg
    25801 ccttttttta tttgaaaaaa ctaatttgag aagaaatttt aactattatg acaataaaat
    25861 tagggtgata ttatataaat aagtaaaatt atattatata atgtatggta tagactttaa
    25921 aaattttatt tgtagagaaa aatatggaca atgttataat aattatagaa attaaaagta
    25981 cttacattca ttatgtatgt gacatgtata attagtttta tatttaatac agttttgaag
    26041 taatatcttc attttgagaa cgttggaata ataaaaaatg tattattata ggtgatataa
    26101 ataaaaaata aatataatat gttatattgc tgatagaaaa gaaaataaaa gaaaataggt
    26161 tttattaaaa gagattttta tttataggac ttaatcaatg tatagagggg tgagaacgta
    26221 tgtttaatgt tgcaattata acacttagtg ataaaggata tgaaggaaaa agagaagata
    26281 taacaggaaa aaagttaact gaatttgttg aaaatacagg agcttataaa gttactgaat
    26341 atgtacttat aaaagatgat aaggaaatgc taaaggaaaa tattataaga ctttgtaata
    26401 gtaataaaat agatttaatt cttacaaatg gaggtactgg tttttcaaaa agagatataa
    26461 ccccagaagc aactaaggaa gtgttggaaa aagaaatacc aggtctgagc gaatatatga
    26521 gaatgaaatc cacagaaatt actaaaaagg ccatactaag taggggtgta agtggaataa
    26581 gaaataattc tataataata aatttacctg gaagtcctaa aggtgcagtt gaaaatctaa
    26641 gttttataat tgatgtttta gatcatggca tagaagtttt aagaggagaa gctacagaat
    26701 gtgccactaa aaaaagtgaa tagtatataa tctagataga taaaaaatag acaaagaggt
    26761 agataaagta tgattgataa gtatggaaga aaaatagact accttcgtat ttcacttaca
    26821 gataagtgta atttaaggtg tgtctattgt atgccaccag atgtaaaatt tgataaaaat
    26881 tatataaatg aaaatctaag ctttgaagac tacaagttta ttataaaggc tatggctgaa
    26941 caaggtataa caaaaattag atttactgga ggagaacctc ttttatatcc taaattaaat
    27001 gagcttataa aatttacaaa agaagaatgt ggaataaata acataggaat gaccactaat
    27061 gctattgggc tttctgatag aatctttgag cttgaaaaat gtggcttaaa taaagttaat
    27121 ataagtttag attctttgaa ggaatataag tataaatccg taacaagagg aggaaactta
    27181 aaagatgtct taaaatcgat tgaatcttgt ttaaacttag gtataaaagt aaagttaaat
    27241 tgcgttgcta taagtgggtt taatgatgat gagcttaaag attttatgtt tttaacagtt
    27301 aaatcaccaa ttgatgtaag atttatagag cttatgccat taggtgaggc taatagagtt
    27361 tataagaagg gttattttaa tgtaaaagaa atgattgaaa aaatatatgg tttatataaa
    27421 gtcaaaaatg atgaaaaaag tacagcagaa tattatatgt ttaaaggagc taaaggaaga
    27481 ataggaatta taactccatt aagctgttca ttttgtaaca cttgtaatcg tattagactt
    27541 acttctagtg gtgcaataaa attatgtcta cattcaaaag aagaaataga tataaaacca
    27601 tttttacata aaccattgtt atttagagaa tctatgaaag atataataaa acaaaaacca
    27661 gaaaaacatc atttaataga aaataaatgt agtgatacaa ataggcgtat gtatgaaatt
    27721 ggaggataag taatgttaac acatataaat gaaaaagggt atgccaagat ggtggacata
    27781 agtgaaaaag atgatactaa aagaacggcc gtagctacag ctacaattac tcttagtaag
    27841 gaagcattag aaaaagtaaa aaatggtcag ataaaaaaag gagacgtatt gtctgtagct
    27901 caagttgcag gtataatggg agctaaaaat acatcttcaa acataccaat gtgccatcag
    27961 attaaattag ttggatgtga tttagaattt gatgtagatg attctatgtc tgctattaag
    28021 atatacgcaa cctgtaaatc tcttgggaaa actggagtag aaatggaagc tttaaatgca
    28081 tgttctttag cagcacttac aatttatgat atgtgtaaag cagttgataa aaaaatggtt
    28141 ataagtaata tccatcttgt ggagaagact ggtggaaaat caggagattt taagttcgag
    28201 taaattaata atatattggg ttaaaatttg gaggaaatat ggcgaaggta gtgtcaataa
    28261 atataagtga gaaaaagggt gtaattaaag ttcctgtacc atgtgcagag ctaaaaataa
    28321 atcatgggat agttggagat gcacatgcag gaaactggca tcgtcagata agcttacttg
    28381 gaaaagaaag catagataaa atgaaaatga aaggctttga ccaattaaaa tatggagatt
    28441 ttgctgaaaa tgtaactaca gaagggatag aagttttttc cctgcctgtt ggtacaaagt
    28501 taagaatagg gcaatgtgaa gttgaagtaa ctcaaatagg gaaaaaatgt cacaatggat
    28561 gtgaaattaa gaaattgaca ggtgactgtg tgatgccacg agaaggaatt tttgtaaagg
    28621 ttctaaaaga aggaattata aatgtgaatg attcaataga tgtaatctaa tgaagtcact
    28681 attaaaatat aat
//
LOCUS       DBGJFF010000040        28609 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.040, whole genome shotgun sequence.
ACCESSION   DBGJFF010000040 DBGJFF010000000
VERSION     DBGJFF010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28609
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.040"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(469..981)
                     /locus_tag="ACOIAF_001792"
     CDS             complement(469..981)
                     /locus_tag="ACOIAF_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668805.1"
                     /translation="MKVKKFKSGIVMALTVMAIGASLTNVSAMELNKSKEFETKYNEM
                     KRDFQEEYGKEDKFNITLDDEFTKEEVNGAKGIIKTTNKMNNEVVVLNYFDEMKSGIE
                     FELKPKAEKNAILRDEFQQGYGKEDKFKVVLDDEFTKEEVNGAKGIIKTTDKETGEVI
                     IYNYFDELEK"
     gene            1534..2415
                     /locus_tag="ACOIAF_001793"
     CDS             1534..2415
                     /locus_tag="ACOIAF_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HGZ2668806.1"
                     /translation="MKKRLIIMILSVVLVLSSILTIFAYSNIKYNNNKLIYSNMIDKK
                     TQNSVKEILKENKINEKDIDTFIKAVNNYNKLQVKILQNNINISKSGYSSINAKQVPY
                     NLEKLQDNWVKKFPDYMDVNCRITAFRLFKDFINSNKKFTGDSIDLNVDLDTIMNNKD
                     AKFSTKDVEKFINFFSAIPAKDTDDTIKIAEQIKNEWKKRKISFKNNKNISIINGFLR
                     YPETKNVFIGHTGICIKTKNGILFLEKYGVTSPYQVTKFKNKEDVKNYMFNRLKMSEG
                     EIELPDPIIMENDKLMK"
     gene            complement(2728..3507)
                     /gene="hisJ"
                     /locus_tag="ACOIAF_001794"
     CDS             complement(2728..3507)
                     /gene="hisJ"
                     /locus_tag="ACOIAF_001794"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ"
                     /protein_id="HGZ2668807.1"
                     /translation="MKDGHIHSPYCPHGSKDDFEKYVQRAMDVGITEMTFTEHFPYAN
                     GFRDPAPENDSCMSIEDLPKYFNDVKKLKEKYEGKIKINVGSEVDFIEGYEEGIKENL
                     NKYGKELEDSLLSVHIIKIDDDYYCVDYSVEEFQNLIDKLGSIEAVYNKYYETLIKAV
                     NSDLGIHKPKRIGHLNLVRKFNQVFPYDYKGNTILEELIKLIKEKGYELDYNVSGNRK
                     EYCKEPYIDGYLLELVKKYDIPLVLGSDSHCAEEINKYDII"
     gene            complement(4053..4403)
                     /locus_tag="ACOIAF_001795"
     CDS             complement(4053..4403)
                     /locus_tag="ACOIAF_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter"
                     /protein_id="HGZ2668808.1"
                     /translation="MIFDKEIVRYHHGHHDHDHCHEHTHGDICHEHPHDHAHDHDHEH
                     SHEESSESKDEKTLKILLVHWINHNETHEEGFREWVEKARAIGKEETAKSIEKAIEYM
                     EEANKMLLEAKKHM"
     gene            complement(4419..4835)
                     /locus_tag="ACOIAF_001796"
     CDS             complement(4419..4835)
                     /locus_tag="ACOIAF_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3842 family protein"
                     /protein_id="HGZ2668809.1"
                     /translation="MIIAVIDGMGGGIGAQIVSSLREELPTYVEIYALGTNSIATSSM
                     MKAHANKGATGENAIVVSAKKANIIVAPISVIIPNSMMGEVTCNISEAIADSEALKIL
                     LPIMPENVELVGLEGKPLALLVKDSVNLIKKEFNIK"
     gene            complement(4854..5045)
                     /locus_tag="ACOIAF_001797"
     CDS             complement(4854..5045)
                     /locus_tag="ACOIAF_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CooT family nickel-binding protein"
                     /protein_id="HGZ2668810.1"
                     /translation="MCESSAFICKSNNELEKVMENVVNIDPCDGKIYLTDLLGEQKII
                     DGIIKEIRLMDHKIIIQEN"
     gene            complement(5196..6011)
                     /locus_tag="ACOIAF_001798"
     CDS             complement(5196..6011)
                     /locus_tag="ACOIAF_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HGZ2668811.1"
                     /translation="MNQWRVIHNKSYEGAMNMAIDEAIFTAYKKGHNKPTLRFYTWEP
                     ACLSIGYFQKLEDEIDLDKCRCMNIDYTRRITGGRAVLHDNELTYSIIIGEDNPLIDK
                     SINLSYRYISEGLVKGLNLSGIETDNLNRGERISRENLSAACFNAHASYEVTINNKKV
                     IGSAQSRKDGVLLQHGSIILDFDVEKLFKLIKTKTPELKERAMKFTAKKASGIENEIG
                     RKIDIDILQKNIVKGLAEQFNVEFVEGDLTDYEKQLVKELYEKYKNEEYNKKR"
     gene            complement(6058..6996)
                     /locus_tag="ACOIAF_001799"
     CDS             complement(6058..6996)
                     /locus_tag="ACOIAF_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HGZ2668812.1"
                     /translation="MIRLSVGTAIELGILNKKSDIPPTTAYIMIGEKCINKCSFCSQS
                     IESSTRKDKLSRVIWPEFSKEEILDALKAYKGKNIKRICIQSMASEEAHNSVLDFINY
                     ISGKIDMPISLSAKLENDEQIKKFFSAGVNKIGIAIDAANKELYEKIKGNNYDEKLKF
                     ITEMSKSYPNKISTHIIVGMGESHEDIYNLYTYLKENDVTISLFAFTPVRGTKMEKIS
                     QPSIESYRRVQLISYMINKGYPKEYFKFKNGYLNSIKLDNDILKDINKGYPFEIRGCK
                     DCNRPYYNERPGSTIYNYSRPLNQSEIDLAIREINL"
     gene            complement(6993..7865)
                     /locus_tag="ACOIAF_001800"
     CDS             complement(6993..7865)
                     /locus_tag="ACOIAF_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HGZ2668813.1"
                     /translation="MNKNLSEKMKIAFDIKLKNFGNKIEFAYPNQTLALSTTSNQCSL
                     KCAHCNGHYLNNMVPIEEYEEKVQSRNITSFLLSGGCSYEGDVPINTHINTIKNLKEQ
                     GYRLNAHLGLMDKDSIVELCKYLDIVSFDLVFDDETIREVYKMKKSKEDYIEVYSTIQ
                     EHTEVAPHICIGLKGGQIKGEYEIIEYLQKNPPNKLTFIVLIPTKGTEYENVEPPELE
                     GVADILCEARINLPDTEINLGCMRPRGVYRKELDQLSIMCGVNRIVLPSRSAKNKAIE
                     MNMTINECKECCVL"
     gene            complement(7890..9818)
                     /gene="acsV"
                     /locus_tag="ACOIAF_001801"
     CDS             complement(7890..9818)
                     /gene="acsV"
                     /locus_tag="ACOIAF_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid activation/regeneration protein AcsV"
                     /protein_id="HGZ2668814.1"
                     /translation="MIKVSFTPNNKEVYCNEGDILLEVARNADIFIDAPCNGNVSCGK
                     CKVKLLNGKVDTEKTRHITDDEWEQGYILACCTKVISDIEIEVPSKVSSSMHGMKIEG
                     SNKKEDREIFERAKKIIEEHNLQFKTNIKKKYIEMEEPNLDDNISDVDRLERYVRNNL
                     GYNEIDFRLDILRKMPTVFRKSDFKVTITYVQKQKKLTIINIEQGNKENSLYGVAIDI
                     GTTSVVVCLVDLYSKEVVDKASSGNAQIKYGADVINRIIYSTKKNGLETLHKAIVEET
                     INPLLKTIYERNGIDKEDVVTLVAAGNTTMTSLFLGVYTDFLRQEPYIPPFLKSPKLM
                     GENVGLFVNDSAYVYLAPSVASYVGGDITAGVLSAGIWSSEENVLFIDLGTNGEIVFG
                     NKDYMMSCACSAGPAFEGGGISCGMRASAGAIEKVIIDKDTLEPTLKIIDECAPVGIC
                     GSGIIDLICQMITKGVIDRRGKIYRDLDNKRVRFNEHEIGEYVLAFKEEFDLENDIVV
                     NEVDIDNFIRAKGAIYSGAYTLVDSLGMDFSILDRVYIAGGIGNNLDIENSIIIGLLP
                     DIDREKFTYIGNSSLVGSYLALISKDAKNKLEEIGNQITYVELSVYPSYMDEFISACF
                     LPHTNIEQFPTAKKLLEE"
     gene            complement(9930..10307)
                     /gene="gcvH"
                     /locus_tag="ACOIAF_001802"
     CDS             complement(9930..10307)
                     /gene="gcvH"
                     /locus_tag="ACOIAF_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HGZ2668815.1"
                     /translation="MKLLPELKYSKDHEWVKVIDGDVVYIGITDYAQDQLGEILFVET
                     PEVEDTVTKGVDFGVVESSKVASDLISPVNGEVLEVNEKLEDEPECINEDPYENWILK
                     VKLADVAELDTLLSDKEYEAGLE"
     gene            complement(10394..12520)
                     /gene="acsB"
                     /locus_tag="ACOIAF_001803"
     CDS             complement(10394..12520)
                     /gene="acsB"
                     /locus_tag="ACOIAF_001803"
                     /EC_number="2.3.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit alpha/beta"
                     /protein_id="HGZ2668816.1"
                     /translation="MNLYNIIFTGSEQALGAAQAMLAEAIEKNGKEHKVAFPDTAYSL
                     PCIYAATGQKMNTLGDLEGALEVVKSLINRTHLLEHAFNAGLATALAAEVIEALKYST
                     MDAPYSEPCAGHITDPIIRSLGVPLVTGDIPGVAVVLGECPDAESAAKVIKDYQSKGL
                     LTFLVGKVIDQAIEAGVKMGLELRVIPLGYDVTSVIHVVSVAVRAALIFGGLTPGDLN
                     GLLEYTANRVPAFVNAFGPLSELVVSAGAGAIALGFPVITDQTVLEVPMNLLTQKDYD
                     KIVATSLEARGIKIKVTEIPIPVSFAAAFEGERIRKSDMFAEFGGNRTEAWELVVKKE
                     ATEVEDHKIEIIGPNIDEVDADGVLRLPLAVIVKIAGKNMQEDFEPVLERRFHYFLNY
                     IEGVMHVGQRDMAWVRISKDAFDKGFRLEHIGEVLYAKMLDEFESVVDKCEITIITDA
                     EKVSELKGEAIAKYNARDERLASLVDESVDTFYSCNLCQSFAPAHVCVVTPERLGLCG
                     AVSWLDAKATKELDPTGPCQPIEKGECLDDRTGVWNSVNETVNQISQGAVESVTLYSI
                     LEDPMTSCGCFECICGIMPEANGFVVVNREFASVTPVGMTFGELASMTGGGVQTPGFM
                     GHGRHFISSKKFAYAEGGPERIVWMPKELKDYVADKLNATVKEMTGIENFCDMVCDET
                     IADDSEGVLAFLEEKGHPALAMESVM"
     gene            complement(12577..13383)
                     /gene="acsE"
                     /locus_tag="ACOIAF_001804"
     CDS             complement(12577..13383)
                     /gene="acsE"
                     /locus_tag="ACOIAF_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase/acetyl-CoA
                     synthase methytransferase subunit"
                     /protein_id="HGZ2668817.1"
                     /translation="MEKFMIIGERIHCISPSIRKALAERDPAPILKRAKEQLEAGAHY
                     IDFNIGPAERDGEEIMTWGIKLLQSEFNNVPIALDTANKKAIEAGLKVYDRTNAKPII
                     NSADAGSRFDLIDIAAEYEAMVIGLCAKEGIPRDNDERMAYCQEILEKGLMLGMEPTD
                     ILFDPLCLVIKGMQEKQVEVLEAIKMMTEMGLLTTGGLSNVSNGCPKHVRPVLDSAFL
                     AMAMANGFSSAIMNPCDPELMKTVKSCDIINGASLYADSFLELNEGGFAF"
     gene            complement(13433..14800)
                     /gene="acsC"
                     /locus_tag="ACOIAF_001805"
     CDS             complement(13433..14800)
                     /gene="acsC"
                     /locus_tag="ACOIAF_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit gamma"
                     /protein_id="HGZ2668818.1"
                     /translation="MALKALDIFKLTPKKNCKDCGFPTCMAFSMKVASGAVEVGKCPH
                     MSDDAIAKLSEATAPLMKALKVGAGASEYELGGETVLFRHEKTLVSRNRYAVSFCTCM
                     SDEAVDAKIANMKKVDYVRIGEQMKVEMAVLEYCGDKDAYLKLIDKIKGSGLEVAYIL
                     ACDDAQVVKEAVEVLKDARPMVYGATKENYKDMIEVVKGASLPLGVKAGSLEELYETV
                     ELIQAAGYKELVLDVTGENIKDTYTNAIQVRRTALKEQDRTFGYPSIVFANRLSNSNP
                     MMEVALSSIFTIKYGSIIVIDDISYAKALPLFALRQNIYTDPQRPMRVEPKIYPINNP
                     DENSPVLVTVDFALTYFIVAGDIERSKVPVWLVIPDAGGYSVLTSWAAGKFGGNSISA
                     FIKESKVEEVTNCKDLIIPGKVAVLKGDIEDNLPGWNVVIGPEESMELPKFLKGYQEK
                     ACQTN"
     gene            complement(14831..15775)
                     /gene="acsD"
                     /locus_tag="ACOIAF_001806"
     CDS             complement(14831..15775)
                     /gene="acsD"
                     /locus_tag="ACOIAF_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit delta"
                     /protein_id="HGZ2668819.1"
                     /translation="MAFKMSTQKYSGKISEVEVGIGEKAIKLGGENVLPFYSFDGEVG
                     NSPKIGIQISDVYPESWTDSYKELYKDVANCPVEWAKYVEANTQADFICLKFDGSDPN
                     GLDKSVDECADVAKAVIEAIKLPLVVAGSGNHEKDGKLFEKLAQTLDGHNCLFMSAVE
                     DNYKGVGASAGMAYAHKVGAESSVDINLAKQLNVLLTQLGVKGENIVMNVGCSAVGYG
                     YEYVASTMDRIRLAAFGQNDKTLQMPIITPVAFEVGHVKEAIAPIEDEPDWGCPEERT
                     IAMEVSTAASVLVGGSNAVILRHPKSIETIKELVNALA"
     gene            complement(15795..16565)
                     /locus_tag="ACOIAF_001807"
     CDS             complement(15795..16565)
                     /locus_tag="ACOIAF_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase accessory protein
                     CooC"
                     /protein_id="HGZ2668820.1"
                     /translation="MGYNIAVAGKGGTGKTSLTGLLIDYLVKDKKGPVLVVDADANAN
                     INEVLGIEVEATIGEIREEVNQREKLGNAFPGGMTKAQYLQFRLNSIIEEGEGYDLLV
                     MGRSEGEGCYCFVNGILREQVNKISGHYKYLVMDNEAGMEHLSRKVTRHVDTLLLVSD
                     CSRRSIQAVARIRDLAEELKLSVGRILLIVNKVPNGVMNDGVKEEIEKHNLELIGVVP
                     MDELIYEYDSTGIPLVNLPEDSKSKVAMKEIFAKLELK"
     gene            complement(16621..18006)
                     /gene="lpdA"
                     /locus_tag="ACOIAF_001808"
     CDS             complement(16621..18006)
                     /gene="lpdA"
                     /locus_tag="ACOIAF_001808"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HGZ2668821.1"
                     /translation="MKIVVVGGGPGGYVAAIKASMLGADVTVVEKRRVGGTCLNAGCI
                     PTKALLASSGVLNTVKEAKDFGIEIDGTVKPNFTAIMERKNKVVNQLISGIEFLFEKR
                     GVNLVNGFGKLIDKNTIEVTKDDGTVETIKADKIILANGSVPVVPRMFPYDGKVVITS
                     DEVLGLEEIPESMLIVGGGVIGCEIGQFFRALGTEVTIVEMVDQILLNEDKDVAKQLL
                     RQFKKDKIKVITGIGVQTCEVVDGKAVATLSNGKVIEAQYALVCVGRRPNLDNSGVED
                     IGIEMERGKVVVNEHLETNVEGIYAIGDIIDTPFLAHVASKEGIVAVENALGKTKVVD
                     YRAIPRCVYTEPEVAGVGKTEKQLEAEGVEYNVGQFDFRGLGKAQAIGHFQGFVKVIA
                     DKETDKIIGAAVVGPHATDLLTELSLAVHLGLTVEQVGDAIHPHPSLSEGLMEALHDV
                     HGECVHSVPKL"
     gene            complement(18050..18931)
                     /locus_tag="ACOIAF_001809"
     CDS             complement(18050..18931)
                     /locus_tag="ACOIAF_001809"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HGZ2668822.1"
                     /translation="MSLLRETLESGKFAVTTEMAPPKGTDLSHLIECAKPLVGRVHAA
                     NVTDFQSAVMRATSLATCKLLKDAGLEPVIQITGRDRNRIAIQGEMLSAGVFGINNLL
                     ALTGDHTSVGDHPQAKGVFDLDSVGILQTAETLMAGTDMAGNKLKGSPDFYLGASVTP
                     EYSPIEVQLLKMKKKIKAGAKFFQTQALYDINTMRKFRELTRDMDCKVLAGIVPLKSP
                     GMAKFMTANVPGIFVPDEQIERLRAAGKENWVSEGIKMAGELIKQLKEEDLCDGVHIM
                     AIGAEENIPAILDAAGL"
     gene            complement(18967..19641)
                     /locus_tag="ACOIAF_001810"
     CDS             complement(18967..19641)
                     /locus_tag="ACOIAF_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HGZ2668823.1"
                     /translation="MIISENKPLEEVLGYLKDFDKLVLVGCNQCAATCKSGGEEEVLK
                     MKETLEGEGKKILGYVMLDPACNLLKSKKDLKALKEETKEADAVLSLACGDGTQTIVK
                     NLKDKPVYPANNTLFIGEVQRVGEYEEACKACGDCELGWTGGICPVTMCAKGLMNGAC
                     GGAKNGKCEVNSENDCAWIKIYERLEAIGQLDNLAEIRPPKDYSKQNNPRSLSAKKKK
                     EAAANS"
     gene            complement(19678..20550)
                     /locus_tag="ACOIAF_001811"
     CDS             complement(19678..20550)
                     /locus_tag="ACOIAF_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HGZ2668824.1"
                     /translation="MEGMSTKGQIIKGKPVADKISEELIKEVDLLIKEGINPKLTIVR
                     VGARSDDLSYERGALKRCQNIGITTEVLELAEDITQEEYIDVLKRVNDDKNVNGILCF
                     RPLPKHLNEEVIKYVIAPEKDVDCFSPINSAKVMEGDKSGFPPCTPTAVVEILKHYNV
                     DLKGSKVTVLGRSMVVGKPVSMLLLSEHATVTICHSKTKNLSGVAAEADVLIAAIGRA
                     KMVDESFVKDGAVVIDVGINVDEEGNLCGDVDTNAVLDKVSMITPVPAGVGSVTTSIL
                     AKHVVKACKLQNNK"
     gene            complement(20642..21274)
                     /locus_tag="ACOIAF_001812"
     CDS             complement(20642..21274)
                     /locus_tag="ACOIAF_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HGZ2668825.1"
                     /translation="MKIADKTCVDFVEVLSSKEAVPGGGGAAALVGAIGMALGSMVCN
                     LTIGKKKYAEYEESVKEILSKAGKLEKDLLKMIDDDAECFLPLSKAYGLPKETEEEKR
                     IKAETMEKALKVACEVPLNIVRVCYEAIKLHEDLVDKGSRLAISDVGVGVQCLRAAIL
                     GGQLNVVININSIQDKEYVNKVKTEVDKLVEDGVKICDEVYAKVEKALGK"
     gene            complement(21379..23055)
                     /locus_tag="ACOIAF_001813"
     CDS             complement(21379..23055)
                     /locus_tag="ACOIAF_001813"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HGZ2668826.1"
                     /translation="MGFKSDIEIAQEAKPQDIREVAKKLGLGEDDVELYGKYKAKVDY
                     NLLKRETGKKAKLILTTAINPTPAGEGKTTTTIGVADAFAKLDKNVLVALREPSLGPV
                     FGVKGGAAGGGYAQVVPMEDINLHFTGDFHAIGAANNLLAAMLDNHIHQGNALRIDPK
                     KITWRRCVDMNDRQLRNIVDGMGKKGDGAVRQDGFDITVASEIMAAFCLASDISDLKE
                     RLGNIIVGYSYEGEPVTARQLKANGAMAALLKDALKPNLVQTLEGTPSFVHGGPFANI
                     AHGCNSVIATRMAMHFADYVITEAGFGADLGAEKFLDIKCRMANLKPDAVIIVATVRA
                     LKYNGGVAKADLNNENLEALEKGLPNLLKHVENITQVYGLPAVVAINRFPLDTEAELK
                     LVEDKCKELGVNVALSEVWAKGGEGGIAVAKEVLRLLDEEENNFRFCYEDDLSIKDKI
                     NAIATKIYGADGVDYTPEADKEIANLEKLGFTKVPVCMAKTQYSLTDDQTKLGRPTGF
                     RITVRQATISAGAGFIVALTGEIMKMPGLPKVPAAEKIDVDENGVIAGLF"
     gene            complement(23304..24083)
                     /locus_tag="ACOIAF_001814"
     CDS             complement(23304..24083)
                     /locus_tag="ACOIAF_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HGZ2668827.1"
                     /translation="MKIAITGKGGVGKTTFSSMLSRMFAEDGYRVVAVDADPDANLAL
                     ALGFPKEVYESIVPISEMKKLVSDRTAASVGSFGKMFKMNPKVDDIPENFCKEYNGVR
                     LLTLGTVDSGGTGCVCPEHVLLKRLCSHLILQNKDVVVMDMEAGIEHLGRGTAQGVDA
                     FIVVVEPGERSLQTYRKVKKLGHDIGVNKVFVVGNKIRNKEDEEFIIQNLEDGESLGF
                     IYYNQDVIDSDRANQSPYDSSETTKEQIKAIKDKLMSLKDK"
     gene            complement(24393..26312)
                     /gene="cooS"
                     /locus_tag="ACOIAF_001815"
     CDS             complement(24393..26312)
                     /gene="cooS"
                     /locus_tag="ACOIAF_001815"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_function: GO:0043885 - anaerobic carbon-monoxide
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006091 - generation of precursor
                     metabolites and energy [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HGZ2668828.1"
                     /translation="MDEKMLTIDLNSQKMIAKAREEGVETMYDRKEGFKAQCGFGLQG
                     VCCRICGMGPCRISPKTPRGLCGADEHTIVGRNFARMVAGGTAAHSDHARDIAHTLAL
                     ADPNGNYKIRDEAKLITLAKEWDVETEGRDIYDVAHEVAEIALMEFGKPFGTLRFIKN
                     APEPRQKIWKEYAIEPRAIDREIATIMHSTHIGCTGDIDSLIHMSLRTSMADGWGGSM
                     IGTRLSDILFGTPVPRRTEANLAVLEENKVNIILHGHEPALSEMIVLASEEPDLVALA
                     KEVGADGINLAGMCCTGNEITMRHGVKIAGDFHQQELAIITGAVEAVIVDVQCIFPAL
                     ARVADCYHTKFVTTSPKAKITGSTYIEFREEQALDDAKAIVKEAILNFKNRDKSKVLI
                     PELKSGATVGYSVEAVVNQLDRVVNSHIDPAGTVKPLTDCLKSGVLRGAAGVVGCNNA
                     KGVSNEAHVTIMKELIKNDIIVVTTGCGASAAAKFGLMESDAAEKYAGKGLATVCKLV
                     GIPPVLHMGSCVDISRILDLVGAAANYLDMDMCDLPVVGIAPEWMSEKAVAIGCYVVA
                     SGIDTYLGIMPPIAGSSRAVDILTSELKDKVGATFTVNTNPKELAATIIEDIEKKRVH
                     FEALVEEKMAEKAEA"
     gene            complement(26919..27830)
                     /locus_tag="ACOIAF_001816"
     CDS             complement(26919..27830)
                     /locus_tag="ACOIAF_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HGZ2668829.1"
                     /translation="MIKKLDDILEQLKGGEKIVLSVAAAHDKEVLMAIKDAVERNIIT
                     PILVGNEGKIREISKEIGFDLSGIKIVDKDDIKECAEIAVKLVSSKEADFVMKGLLDT
                     SVILKEVLNKDYGLRTDSLLSHVMVYELEKYHKLLITTDGGMNIAPDYEQKAKILKNS
                     IKAAKALGMETVKVACLAAKEKVNPKMQATVDADMLAKACKEGEFGENVIVEGPLAFD
                     LAVSKEASEIKGFKSEVSGDVDIILVPTIEVGNGIGKAFTYMADSKSAGIIMGAKAPI
                     VLVSRADSHESKLYSIAYGAIVAKNMK"
     gene            complement(27946..28524)
                     /locus_tag="ACOIAF_001817"
     CDS             complement(27946..28524)
                     /locus_tag="ACOIAF_001817"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HGZ2668830.1"
                     /translation="MNINEFLTHINQRKPVSSKSEIHEFMVKLSNEAMKITSKLNSSY
                     HEPEEVRSLFSELIGKKVDESFFMFPPFYTDCGKNITIGKNVFINSSCHFQDQGGIEI
                     GDNTQIGHNVVLATLNHGIAPEKRGTTYPSPITIGKNVWIGANVTVVPGVTIGDNAII
                     AAGAVVTKNVAENTIVGGVPAKLLRVIDEIPE"
BASE COUNT         8571 a         5694 c         3185 g        11159 t
ORIGIN      
        1 tctattattg tttgttttta acacaatcgt tttttgttga atcattttat attcttgata
       61 caatcattct tgcattctat tattgtttat ttttgacgca atcgttttta ttcagtcatt
      121 ttatattctt gatacaatca ttcttacatt ctattattat ttgtttttga cacaatcgtt
      181 ttttattcag tcattttata ttttcgacac aatcattctt acattctatt attgtttgtt
      241 ttgatacaac tgtttttatt cagtcatttt atattttcga cacaattatt cttacattct
      301 attattgttt atttttgacg caatcgtttt ttatttagtc attttatatt ttcgacacaa
      361 tcattcttac attctattat tgtttgtttt gatacaactg tttttattca gtcattttat
      421 attctcaaca caattacctt ttcattcaat caaagtaatc tttaagcatt atttttcaag
      481 ctcatcaaag taattatata tgataacctc acctgtctct ttatctgtag tctttataat
      541 gcccttagct ccatttacct cttcttttgt aaattcatca tctagcacaa ctttgaattt
      601 atcttccttt ccataacctt gttgaaattc atctctcaaa attgcatttt tttctgcttt
      661 tggcttcaac tcaaattcaa ttccagattt catttcatca aaataattta atactacaac
      721 ctcattattc attttattag ttgtctttat aatcccttta gctccattta cttcttcttt
      781 tgtaaattca tcatccaatg ttatattaaa tttgtcttct tttccatatt cctcttgaaa
      841 atccctcttc atttcattgt atttagtttc aaactcttta ctcttgttta attccatagc
      901 tgatacattt gtcaatgatg ctccaattgc cattactgtt aatgccatta ctataccact
      961 tttaaatttt tttactttca tatcttacct cctaaattaa atgttctcaa tgaaattata
     1021 taaagaaaat cctaagtttt acagtttcat tcttgtaact aaaattacta ttatgtaacc
     1081 aaactttttg cttcaactat tccttaaaag ttgctttttt gaggaatagt tgaagttaaa
     1141 taattagatt ttatctatat ttatagattt aaaatcaaat ttatccatat aaataaatac
     1201 ttatttttgc aaaataaaaa tttttaatta ctccaatcct tcttatttac ttaaaatcct
     1261 atgataagaa atttaaaagt ctttaccttc tttaacaata atttatttac ttttaacata
     1321 caaaatatat agagtatatc aaaactattt tactgaatat tctaaaaata agtaccccct
     1381 attagtatat atatttacat attaaaaaaa agttttaaat aaagttatat tttttacaac
     1441 agttttgtaa ttaaaaacat attgaaggta aatttaaagg tactatatac tataaatatg
     1501 tcgacacaat aaatttaaaa aggagaacta aatatgaaaa aaagattaat aataatgata
     1561 ttgtctgttg tattagtact atcaagtata cttacgatat ttgcatattc aaatattaaa
     1621 tataataata ataaattgat atattctaat atgatagata aaaaaactca aaatagtgta
     1681 aaggaaatcc taaaagaaaa taaaattaat gaaaaagaca tagatacatt tattaaagca
     1741 gtcaacaatt ataataaatt gcaagttaaa atacttcaaa ataatataaa tatatctaaa
     1801 tctggatatt cttcaatcaa tgcaaaacaa gttccatata acctagaaaa attgcaagat
     1861 aattgggtga aaaaattccc tgattatatg gatgtaaatt gtagaataac tgcttttaga
     1921 ctttttaaag attttataaa ttcaaataag aaatttactg gagactctat tgacttgaat
     1981 gtcgacttgg acactataat gaataataaa gatgctaaat ttagcaccaa agatgtagaa
     2041 aaatttatta acttcttttc tgcaatacca gctaaagaca cagatgatac aatcaaaatt
     2101 gcagaacaaa taaaaaatga atggaaaaaa agaaaaatat cttttaaaaa taataaaaat
     2161 atatctatta taaatggatt tcttcgttat ccagaaacta aaaacgtatt tataggtcat
     2221 acaggcatat gtattaaaac taaaaatggc attctatttt tagaaaaata tggtgttaca
     2281 tcaccttatc aagttactaa atttaaaaat aaagaagatg taaaaaatta tatgtttaat
     2341 agattgaaga tgtcagaagg tgaaattgaa ttacctgacc ctataataat ggaaaacgat
     2401 aaacttatga aataataact ttaaacgcaa aatagtattt ataattacta agtcaatata
     2461 atagagaagg tctatagatt tgtgtaaata caagcttatc tacaatctta tataagaatg
     2521 tttatataaa taagatagat taattctaag tcctattatt ctattgaatt ttattattat
     2581 tttatttaga ttctattgtt attttactta gaacttatta ttattttatt taagttctat
     2641 tactattttc aatcatataa atttacaaaa gactgtacat aaatttattt tatacaaact
     2701 tatctacagt cttttgtgtg aatatatcta aataatatcg tatttattta tttcctctgc
     2761 acagtgagaa tcagaaccta aaactaatgg aatatcgtat ttttttacca actctaataa
     2821 gtatccatct atataaggct ctttgcagta ctcttttcta tttccagaaa cattataatc
     2881 aagttcataa cctttttctt taattaactt tattaactct tcaagaattg tatttccttt
     2941 ataatcgtat ggaaatacct gattaaactt tctaactaaa tttaaatgac ctattctttt
     3001 tggtttatgt atacctaaat ctgaatttac agcttttatc aatgtttcat aatacttatt
     3061 ataaacagct tcaatacttc ctaatttgtc gattaaattt tgaaactctt ctacactgta
     3121 atctacacaa taataatcat catcaatttt aattatatgt actgataaaa gagagtcttc
     3181 taattccttt ccatatttat ttaaattctc ctttatccct tcttcgtatc cttctataaa
     3241 atccacttcc gacccaacat ttatcttgat tttaccctca tacttttctt tcaacttctt
     3301 gacatcatta aaatactttg gtaaatcttc aatactcata caactatcat tttcaggagc
     3361 agggtctcta aatccatttg catacggaaa atgttctgta aatgtcattt ctgttattcc
     3421 aacatccatt gctctttgta catatttttc aaaatcatcc ttacttccat gaggacaata
     3481 aggtgaatgt atatgaccat ctttcataaa taattcactc cttttttaaa gaaaaaatct
     3541 aatataaatg cataaatctt ttaacttcaa aataatattt ttaatcatgc taatttttat
     3601 tatatctaat attagagtaa ttttaaactg ccttttaagt atcttatcat tagtactatt
     3661 ttcaacttaa cattaatatt attttttaac ttaacattaa tactatcttt aaatagtaat
     3721 actcaatata taattactta acataaaata actcaaattg cttgatgagg cttttgcagt
     3781 atttaaagaa tatataatta tttaacatta aataatccaa tcttgtaata aaatttactc
     3841 attaaagtat attacagaaa atacctcaaa ataaactttt tagttcatga tatactcttt
     3901 tttgcatgaa attagatata tttttatcat ttcaacaatg aaagggaggc ttatatctat
     3961 ctttagacaa ccccttagat aaaattttta ataattaaac atcacaaaat ataaaaatcg
     4021 cttatatcaa acgtataagc gattaattat atttacatat gttttttagc ctctaacaac
     4081 attttattag cttcttccat atactctata gccttttcaa tactctttgc tgtttcttct
     4141 tttccaatag ctctagcctt ttcaacccac tctctaaatc cttcttcatg agtttcattg
     4201 tgatttatcc aatgtactaa aagtattttt aaagtttttt catccttaga ctctgatgat
     4261 tcctcatgac tatgttcgtg gtcgtggtcg tgagcatggt catgtggatg ttcatgacat
     4321 atatctccat gagtatgttc gtggcagtgg tcatggtcat gatgtccatg atgatatctt
     4381 acaatctctt tatcaaaaat cattagttat tccccctgtt attttatatt aaactctttt
     4441 tttataaggt ttacagaatc ttttacaagt agtgctaatg gtttaccttc taatccaact
     4501 aactccacat tttctggcat aataggtaaa agtatcttaa gtgcctcact gtctgcaata
     4561 gcttcactta tattacaagt aacttctccc atcatagaat taggtattat tacagaaata
     4621 ggtgcaacta ttatatttgc ttttttagct gatactacta tagcattttc ccctgtagct
     4681 cctttattag catgagcttt catcatagaa ctagttgcta tagaattagt ccctagtgca
     4741 taaatttcta catatgtagg taactcttct ctcaaagaag aaactatctg ggctccaata
     4801 cctccaccca ttccatctat cactgctatt atcatatgaa attcccctat taattaattt
     4861 tcttgtatta ttatcttatg gtccatcaat cttatttctt taattatccc atctattatc
     4921 ttttgctctc ctaataaatc cgttaaataa atctttccat cacaagggtc tatattaacc
     4981 acattttcca taaccttttc taactcatta ttacttttac aaatgaaagc tgatgattca
     5041 cacatataaa tctcctccta atatgatttt tattaaaaat caaaaaaaga ttcccttagg
     5101 tatttcaata caccaaagaa accttcatga tttcagtttt tgtatattat tataccttaa
     5161 gtatatcact ttaatttatg taaagtcaat taaagttaac gctttttatt atactcttca
     5221 ttcttgtatt tttcatatag ttctttaact aattgcttct cataatcagt caaatcgccc
     5281 tccacaaact caacattaaa ttgttcagct aatcccttta ctatgttttt ctgtagaata
     5341 tctatgtcta tttttctgcc aatttcattt tctattccac ttgctttttt agctgtaaat
     5401 ttcatagctc tttctttcaa ttctggtgtc ttagttttta ttaatttaaa caatttttct
     5461 acatcaaaat ccagtattat tgaaccatgt tgtaataaaa caccatcttt tctactttga
     5521 gcacttccaa ttaccttctt attattaata gtaacttcat atgatgcatg tgcattaaaa
     5581 catgcagctg ataaattttc cctacttatt ctctcgcctc tatttaaatt atctgtttct
     5641 ataccactta gattcaaccc tttaactaga ccttcactta tatatctata agataggttg
     5701 atgctcttat cgattaaagg attatcttct cctataatta tactataggt tagctcatta
     5761 tcatgtaata cagctcttcc acctgtaatt cttctagtat agtctatatt catacatcta
     5821 cacttatcta aatctatctc atcttccaat ttctgaaaat atcctatact caaacaagct
     5881 ggttcccatg tataaaatct aagagttggc ttattatgtc ccttcttata tgcagtaaat
     5941 atagcttcgt caattgccat attcattgct ccttcatatg atttattatg tataactctc
     6001 cactgattca tgattattac gcccccattt ttagtataaa tatatcctag aaatcctcta
     6061 tagatttatt tctcttattg ctaaatctat ctcactttga tttaaaggtc ttgaatagtt
     6121 gtatatagta cttcctggtc tttcattata gtatggtcta ttacaatcct tacaacctct
     6181 tatttcaaaa ggatagcctt tgtttatgtc ttttaaaata tcattgtcaa gctttatact
     6241 atttaagtat ccatttttaa acttaaaata ctcttttgga taacctttat ttatcatata
     6301 agatattaac tgtacccttc tataactttc tatgcttggt tgacttatct tttccatttt
     6361 agttcctcta actggagtaa atgcaaataa actaatcgtt acatcatttt cctttaaata
     6421 agtatataaa ttgtatatat cttcatgact ttcacccata ccaactatta tatgtgtact
     6481 tattttattg ggatatgatt ttgacatttc agttataaat tttaactttt catcatagtt
     6541 atttcctttt attttttcat atagttcttt atttgctgca tctattgcta ttcctatttt
     6601 atttactcct gctgaaaaaa acttctttat ctgttcatca ttttctaatt ttgcagatag
     6661 tgaaattggc atgtctatct taccacttat atagtttata aagtctaaaa cagagttatg
     6721 tgcttcttca cttgccattg attgaataca tattctcttt atattttttc ctttataagc
     6781 ttttaaagca tctaatattt cttcttttga aaactctggc caaattactc ttgataattt
     6841 atcttttcta gtactacttt ctattgattg agaacaaaaa ctgcatttat ttatgcattt
     6901 ttctccaatc attatatatg ctgtggtagg aggtatatcg ctttttttgt ttaatatccc
     6961 aagttctatt gctgtcccaa ctgatagtct aatcataaaa cgcaacactc cttacactca
     7021 tttattgtca tattcatttc tatagcctta ttctttgcag accttgatgg taatactatt
     7081 ctattgactc cacacattat agataattgg tctagctctt ttctatacac tcctcttggt
     7141 ctcatacaac ctagattaat ttcagtatct ggtaaattaa ttctagcttc acataaaata
     7201 tctgctaccc cttctaactc aggaggttct acattttcat actcagtccc ttttgttgga
     7261 ataaggacta taaatgtaag cttattagga ggattttttt gtaggtattc tataatttca
     7321 tattcaccct ttatttgtcc tccttttaaa ccaatacata tatgtggtgc tacttctgta
     7381 tgttcttgta ttgtactata gacttcaata taatcttctt tactcttttt catcttataa
     7441 acttctctta ttgtctcatc atcaaacact aaatcaaaag aaactatatc taaatactta
     7501 cacaactcta caatgctatc tttatccata agacctaaat gagcattcaa tctatatcct
     7561 tgctctttta agtttttaat ggtattaata tgagtattta tagggacatc tccctcataa
     7621 ctacaaccgc cactcaatag aaagctagtt atattccttg attgtacttt ttcctcatat
     7681 tcttctattg gcaccatatt gtttaagtag tgaccgttgc aatgggcaca tttcaaacta
     7741 cattgattgc ttgtagtact aagagctagt gtctggttag gataagcaaa ctctatttta
     7801 tttccaaaat tctttaattt aatatcaaat gctattttca ttttttcaga tagattctta
     7861 ttcatagttt attccttctt aagtacattt tactcttcta gtaatttctt tgctgttggg
     7921 aactgctcta tattcgtatg tggtaagaaa catgcagaaa taaattcatc catataactt
     7981 ggatatacac taagttcaac ataagttatt tgattaccta tttcttcaag tttatttttt
     8041 gcatctttac ttataagagc taagtaagaa ccaacaagtg aactattccc tatataagta
     8101 aatttttctc tatcaatatc aggaagtaac cctataatta ttgagttttc tatgtctagg
     8161 ttgtttccta taccacctgc tatataaacc ctatctaata tactaaaatc catacctaaa
     8221 ctatctacaa gagtgtatgc gcctgaatat atagctcctt tagctcttat gaaattatct
     8281 atatcaactt cattgactac tatatcattt tctaaatcaa attcttcttt aaatgctaat
     8341 acatactctc ctatctcatg ctcattaaat ctaactctct tattatctaa atccctgtat
     8401 attttacctc ttctatctat tacaccttta gtaatcattt gacagattaa atcaattata
     8461 ccagaaccac atataccaac tggtgcacat tcatcaataa tctttaatgt tggctctaaa
     8521 gtatctttat caataattac tttctctatt gctcctgctg atgctctcat tccacagctt
     8581 atccccccac cttcaaaagc aggtcctgct gaacatgcac agctcatcat ataatcttta
     8641 tttccaaata caatctctcc attagttcct aaatctataa ataaaacatt ttcttcactt
     8701 gaccatatcc ctgctgataa tacacctgct gttatatctc ctcctacata acttgcaaca
     8761 gatggagcta agtacacata tgcactgtca tttacaaaaa gaccaacatt ttcacccata
     8821 agttttggag attttaaaaa tggtggtatg taaggttctt gtcttaagaa gtctgtatac
     8881 acacccaaga ataaacttgt catagttgta ttcccagcag ccactaatgt aactacatct
     8941 tccttatcta ttccatttct ttcatatatt gtttttaata gtggatttat agtttcctca
     9001 actatagctt tatgaagtgt ctctaatcca tttttcttag ttgaatatat aattctattt
     9061 ataacatctg ctccatattt tatctgtgca tttccagaag atgctttatc tactacctct
     9121 tttgagtata ggtctactaa acaaacaact actgaagttg taccaatatc tattgcaact
     9181 ccgtataagg aattttcttt atttccttgt tctatattta tgattgtaag tttcttttgt
     9241 ttttgaacat atgttattgt aaccttaaaa tcactctttc taaatacagt tggcattttt
     9301 ctaagtatat ccaatctaaa atctatttca ttatatccta ggttatttct aacatatctt
     9361 tccaatctat ctacatcact tatattatca tctaaatttg gttcttccat ttctatgtat
     9421 ttctttttta tatttgtttt aaattgaagg ttatgttctt caattatttt tttagctctt
     9481 tcaaaaattt ccctatcctc ttttttatta ctaccttcta tcttcatacc atgcatagaa
     9541 gatgatactt tagaaggaac ttcaatttct atatctgata ttacctttgt acaacaagct
     9601 agtatataac cttgctccca ctcatcatct gttatatgtc ttgttttttc tgtatcaact
     9661 tttccattca ataattttac tttacacttc ccacaagaca cgtttccatt acaaggagca
     9721 tctatgaata tatctgcatt tcttgcaacc tctagtaata tatccccctc attacaataa
     9781 acctctttat tgtttggtgt aaaacttact tttatcatgt tatccctccc aatttgttct
     9841 atatatgata aaaggaaagt atagctaaat ttgcgtgcac tcaaatttta ctatactttc
     9901 cccaattata ataatatctt aatctttaat tattctaatc cagcctcgta ttctttatca
     9961 cttaataacg tatcaagttc agccacatca gctagtttta cttttaatat ccagttttca
    10021 tatgggtctt catttataca ttctggctca tcttctaatt tttcattaac ttccaatacc
    10081 tcaccattaa caggagatat taaatcagaa gctactttag aagactcaac tactccaaaa
    10141 tctactccct tagttacagt atcttctact tctggtgttt caacaaacaa tatttctcct
    10201 aattgatctt gagcatagtc tgttatacct atatatacaa catctccatc aattactttt
    10261 acccactcat gatctttaga gtattttaat tctggtaata atttcatttc gatagacccc
    10321 caatattata ttattgatta aattacttaa atcatattaa tattatatca tattatttta
    10381 aattttccat caattacatt acactttcca tagctaatgc tggatgacct ttttcttcta
    10441 agaaagctaa tactccttca gaatcgtcag ctatagtttc atcacaaacc atatcacaga
    10501 agttttctat tccagtcatt tctttaactg tagcatttaa tttatcagct acataatctt
    10561 ttaattcttt tggcatccaa actattcttt ctggtccacc ttctgcataa gcgaattttt
    10621 tagaagatat gaaatgtctt ccgtgtccca taaatcctgg agtttgaact cctccacctg
    10681 tcatagaagc aagttctccg aaagtcatac caactggagt aacacttgca aattctctgt
    10741 taactacaac aaatccattt gcttctggca ttataccaca gatacattcg aagcatccac
    10801 aagaagtcat tgggtcttct agtatagagt ataatgtaac agattcaact gcaccttgag
    10861 atatttggtt tacagtttca tttactgaat tccatacacc tgttctatca tctaagcact
    10921 cgcctttttc tattggttga caaggacctg ttgggtctaa ttctttagta gccttagcat
    10981 ctaaccagct aactgctcca caaagaccaa gtctttcagg agtaacaaca caaacgtgag
    11041 ctggtgcaaa tgattgacat aagttacaag aatagaaagt gtcaacactt tcatcaacta
    11101 atgaagctaa tctttcatct ctagcattgt acttagctat tgcttctcct tttaattcag
    11161 aaactttttc agcatctgta attatagtta tttcacactt atcaactact gattcaaatt
    11221 catctagcat cttagcatat aaaacttctc ctatatgctc aagtctaaat cctttatcaa
    11281 atgcatcttt agagattctt acccaagcca tatctctttg tccaacgtgc attacacctt
    11341 ctatatagtt taagaagtag tggaaacgtc tttcaagaac tggctcaaaa tcttcttgca
    11401 tatttttacc agctatttta actattacag caagtggtaa tcttaataca ccatctgcat
    11461 caacttcatc tatatttggt cctattattt ctatcttatg gtcttcaact tctgttgctt
    11521 ctttcttaac aacaagctcc caagcttcag ttctgtttcc accaaactca gcaaacatat
    11581 cgctctttct aattctctca ccttcaaatg ctgctgcaaa tgaaactggg ataggtattt
    11641 cagtaacttt tatttttatt cctctagctt ctaatgaagt agctactatc ttatcgtaat
    11701 ctttttgagt taataggttc attggaactt caagaactgt ttggtcagtt ataactggga
    11761 atcctaaagc tattgctcca gcacctgcag atacaactaa ttcacttaat ggtccaaatg
    11821 cattaacaaa tgcaggaact ctattagctg tgtactctaa taatccgttt aaatcaccag
    11881 gagttaatcc accgaatatt aatgctgctc ttactgcaac agatacaacg tgtataacag
    11941 aagttacatc atatcctaga ggtataactc ttagctcaag acccattttt actccagctt
    12001 ctatagcttg gtctataact ttaccaacta agaaagttag taaaccttta gattggtaat
    12061 cttttataac ttttgctgct gattctgcat ctggacactc accaagaaca actgcaacac
    12121 caggtatatc tcctgtaact aatggtacac caagtgatct gattataggg tcagttatat
    12181 gacctgcaca tggctcacta tatggagcat ccatagttga atattttaat gcttcaatta
    12241 cttctgctgc taaagctgta gctaaaccag cattaaaagc atgttctagt aaatgagttc
    12301 tatttattaa agattttact acttctaaag caccttctaa gtcccctaaa gtgttcatct
    12361 tttgtcctgt tgcagcatat atacaaggta atgaatatgc tgtatcaggg aaagcaactt
    12421 tatgctcttt tccattcttt tctatagctt cagctaacat agcttgagcc gcacctaaag
    12481 cttgttctga ccctgtaaag attatattat atagattcat tctatttccc cctttaatat
    12541 ttacacctat tttttattaa ctatacccta aaaaccctag aaagcaaatc caccttcatt
    12601 taactctaag aaagagtctg catataaaga tgccccgttg attatgtcac aagattttac
    12661 agttttcatt aattctgggt cacatggatt cattatagct gaactaaatc cattagccat
    12721 tgccattgct aagaatgcac tatctaaaac aggtctaaca tgcttaggac atccattaga
    12781 tacgttagat aatcctcctg tagttaaaag tcccatctca gtcatcattt ttatagcttc
    12841 taaaacttct acttgttttt cttgcatacc ttttataact aagcataatg ggtcaaatag
    12901 tatatcagtt ggctccattc ctaacattaa gcctttttct aatatttctt ggcagtaagc
    12961 catacgctca tcattatctc ttgggatacc ttcttttgca cataaaccta taaccattgc
    13021 ttcgtattca gctgctatat ctattaaatc aaatcttgaa ccagcatcag cagagtttat
    13081 tattggtttt gcattagttc tatcataaac ttttagtcca gcttcgatag cttttttatt
    13141 agctgtatct aatgctatag gaacattgtt gaactcagat tgaagtaatt tgattcccca
    13201 tgtcattatt tcttcgccat ctctttcggc aggtcctata ttaaaatcta tatagtgtgc
    13261 tcctgcttct aattgctctt ttgctctttt taagattgga gctgggtctc tttctgctaa
    13321 tgcttttctt attgaaggtg atatacaatg tattctctca cctataatca taaatttttc
    13381 catttataat gtcccccttt aaatttaata ttaatagtta gttaatattt tattagtttg
    13441 tttggcatgc tttttcttgg tatcctttta agaacttagg taattccata gattcctctg
    13501 gtcctataac tacattccat cctggtaagt tatcttctat atcaccttta agtactgcaa
    13561 cttttcctgg aatgataagg tctttacaat tagttacttc ttctacctta ctttctttaa
    13621 tgaaagcaga gattgagttt ccaccaaatt taccagcagc ccaagatgta agaactgaat
    13681 atccacctgc atctggtatt actaaccata ctggaacttt tgatctttct atatcaccag
    13741 caactatgaa gtaagttaat gcaaagtcaa cagtaactaa tactggagaa ttttcatctg
    13801 ggttgttgat tggataaatc tttggttcaa ctctcattgg tctttgtggg tcagtgtata
    13861 tgttttgtct tagtgcaaat aatggtaatg ccttagcata actaatatca tctattacta
    13921 ttatagaacc gtattttata gtgaatattg atgataaagc aacttccatc atagggtttg
    13981 aattagataa tctatttgca aatactattg aaggatatcc aaatgttcta tcttgctctt
    14041 ttaaagctgt tcttctaact tgtatagcat tagtgtaagt atcttttatg ttttctcctg
    14101 ttacatctaa tactaattct ttatatccag cagcttgaat taattcaact gtttcatata
    14161 attcttctaa gctaccagct tttacaccta atggtaaact tgctccttta actacttcta
    14221 tcatatcttt gtagttctct ttagttgctc catataccat tggtctagca tcttttaata
    14281 cttcaacagc ttctttaact acttgtgcat catcacaagc taaaatataa gctacttcta
    14341 atccactgcc ttttatttta tctattaatt ttaaatacgc atctttatct ccacaatact
    14401 ctaaaacagc catttcaact ttcatttgtt caccaattct tacatagtca acttttttca
    14461 tgttagctat tttagcatct actgcttcat cactcataca agtacagaat gaaactgcat
    14521 atctatttct acttactaat gttttttcat gtctaaataa tactgtttct ccacctagtt
    14581 catactcaga tgcaccagca ccaactttta aagctttcat taaaggtgca gtagcctctg
    14641 ataattttgc tatagcgtca tcagacatat gtgggcattt acctacttct acagctcctg
    14701 aagcaacctt catagagaaa gccatacaag ttgggaatcc acaatcctta cagtttttct
    14761 ttggtgttaa tttaaatata tctaaagctt ttaatgccat tattttttcc ccctaacctt
    14821 aacaatttaa ttaagctaat gcgttaacca attcttttat agtttctatt gattttggat
    14881 gacgaagtat aactgcgttt gaaccaccta ctaaaacact tgctgcagtt gaaacttcca
    14941 ttgctatagt tctttcttct ggacaacccc aatctggctc atcttctatt ggagctatag
    15001 cttctttaac atgacctact tcaaaagcta ctggtgttat gataggcatt tgtaatgtct
    15061 tatcattttg accaaatgct gcaagtctaa ttctatccat agtagatgca acgtactcat
    15121 atccataacc aactgctgaa catccaacat tcataactat attttcacct ttaacgccta
    15181 gttgagttaa caatacgttt aattgtttag ctaggtttat atcaacagaa gattcagcgc
    15241 ctactttatg tgcataagcc ataccagctg atgctcctac tcctttgtag ttgtcttcta
    15301 ctgctgacat aaataagcag ttatgtccat ccaatgtttg agctaatttt tcaaataatt
    15361 taccatcttt ttcatgattt cctgaaccag ctacaactaa aggtaatttt attgcttcaa
    15421 ttactgcctt agctacatca gcacattcat caactgactt atctaatcca tttgggtcag
    15481 aaccatcaaa ttttaaacaa ataaaatctg cttgtgtatt agcttcaaca tatttagccc
    15541 actctactgg acaattagct acatctttgt ataattcctt atatgaatca gtccagcttt
    15601 caggataaac gtctgatatt tgtatcccta tttttggaga attacctact tctccatcaa
    15661 aactataaaa aggtaataca ttttccccac ctaatttaat tgctttttcc cctattccta
    15721 cttcaacttc tgatattttt ccagaatatt tttgagtaga cattttaaat gccatatttt
    15781 tttccccctt aagcctattt taattctaat ttagcaaaaa tttctttcat agccacttta
    15841 gattttgaat cttcaggtaa attaactaaa ggaatacctg tagaatcata ttcatatatc
    15901 aattcatcca taggtacaac accgattagc tctaggttat gcttttcaat ttcttcttta
    15961 acaccatcat tcataacccc atttggaact ttatttacaa tcaaaagtat tcttccaaca
    16021 cttaacttta gttcctcagc taaatctctt attctagcaa cagcttgtat gctacgtctt
    16081 gagcaatcac taaccaataa aagtgtatca acatgtctag tgacttttct acttagatgt
    16141 tccataccag cttcgttgtc catgactaag tatttgtaat gaccagatat tttgtttact
    16201 tgttctctaa gtatcccatt tacaaaacag taacatcctt caccttctga cctacccatt
    16261 actaataagt catatccttc gccttcttcg attatagaat ttaatctaaa ttgaagatat
    16321 tgtgcctttg tcataccacc agggaatgca ttgcctaact tttctctttg atttacttct
    16381 tctcttattt ctcctattgt tgcttcaact tcgataccta atacttcatt tatgttagca
    16441 ttggcatcag catctacaac taatactggt ccctttttgt ccttaaccaa ataatcaatc
    16501 aaaagccctg tgagacttgt tttaccagtt ccaccttttc ctgcaacagc tatattgtat
    16561 cccattttaa gacttccccc ttaaatctag ccacagagcc ttatgtactc tgtctgtcta
    16621 ttataattta ggtacagaat gaacacattc tccatgtaca tcatgaagtg cttccattag
    16681 cccttcagat aaacttggat gtggatgtat tgcatcccct acttgttcta ctgttaatcc
    16741 tagatgaaca gcaagtgaca actctgtcaa taaatctgta gcatgaggtc ctactactgc
    16801 tgcacctatt atcttatcag tttctttgtc agctataact tttacaaatc cttggaaatg
    16861 tcctatagct tgtgcttttc caagacctct aaaatcaaat tgacctacat tgtattctac
    16921 accttcagct tcaagttgct tttcagtctt accaacacca gcaacttcag gttcagtata
    16981 aacacatctt ggaattgctc tataatctac taccttagtc ttacctaaag cattttcaac
    17041 tgctacgata ccttctttag aagcaacatg tgctaggaaa ggagtatcta ttatatcgcc
    17101 tattgcgtat ataccttcta cattagtttc aagatgttca ttaactacta cttttcctct
    17161 ttccatttca attcctatat cttctactcc agaattgtca aggttaggtc ttctaccaac
    17221 acatactaat gcatattgtg cttctataac ctttccatta gaaagagttg caacagcctt
    17281 accatcaact acttcacaag tttgtactcc aatacctgta ataaccttaa ttttatcttt
    17341 cttaaattgt ctaagtagtt gtttagctac atctttgtct tcatttaata gtatttggtc
    17401 aaccatctca actatagtta cttctgttcc taaggctcta aagaactgcc ctatctcaca
    17461 tcctataact ccaccaccta ctattaacat agactcaggt atctcttcaa gtccaagaac
    17521 ttcatcacta gttattacaa cttttccatc atatgggaac attcttggta caactggaac
    17581 agaaccattt gctagtatta tcttatctgc ttttatagtt tctactgttc catcatcttt
    17641 agttacttct atagtgtttt tatcaattaa ttttccaaaa ccattaacta agtttactcc
    17701 tcttttttca aataagaact ctataccact tattaattgg tttacaactt tattttttct
    17761 ttccattatg gcagtgaagt ttggcttaac tgtaccatct atttctatac caaaatcctt
    17821 tgcttctttt acagtattca atactcctga agaagcaaga agtgctttag ttggtataca
    17881 tcctgcattt aagcaagttc ctccaactct tcttttttca acaactgtta catctgcacc
    17941 aagcatagaa gcttttatag ctgctacata tcctcctggt ccaccaccta ctactactat
    18001 cttcatcttt aattccccca aacctaatat aatctacaat cttgcaatac tataaacctg
    18061 cggcatccag tatagcaggt atattctctt ctgccccaat tgccattata tgaacaccat
    18121 cacataaatc ttcttctttt aattgtttga taagttcccc tgccattttt attccttcac
    18181 ttacccagtt ttcttttcca gctgctctta atctttcgat ttgttcatct ggaacaaata
    18241 ttcctggtac atttgcagtc ataaatttag ccattcctgg tgattttaaa ggtactattc
    18301 ctgctaatac cttgcaatcc atatcccttg ttaattctct aaactttctc attgtattta
    18361 tgtcataaag tgcttgtgtt tggaaaaatt tagcccctgc ttttattttc tttttcattt
    18421 ttaataattg aacttctatt ggagaatatt ctggagttac tgatgctcct agataaaaat
    18481 ctggagaacc ttttaactta ttaccagcca tgtccgtacc tgccattaaa gtctcagcag
    18541 tttgaagtat ccccacagaa tctaaatcaa acactccctt agcttgtgga tggtctccta
    18601 cgctagtatg gtctcctgtt agtgccaaca gattgtttat cccaaataca cctgctgaca
    18661 acatttctcc ttgtatagct attctatttc tatctctccc tgttatttga ataactggct
    18721 ctaatccagc atcttttaat aatttacatg tagcaagaga agttgctctc ataactgccg
    18781 attgaaagtc tgttacattt gctgcatgaa ctcttccaac taacggcttt gcacattcta
    18841 ttaaatgtga aaggtcagtc ccttttggag gagccatttc agtagtaact gcaaattttc
    18901 cactttctaa tgtttctctt aataagctca tctctaatcc cccttttaac cttctaaaaa
    18961 aaacttttat gaatttgctg ctgcttcttt ctttttcttt gcacttaaac ttcttggatt
    19021 attttgctta gaatagtctt ttggtggtct tatttctgct aagttatcta attgtcctat
    19081 agcttctagt ctttcatata tctttatcca agcacaatca ttctctgagt ttacttcaca
    19141 cttaccattt ttagctccac cacaagcccc attcataaga cctttagcac acatagttac
    19201 tggacatatc ccacctgtcc aaccaagttc acagtctcca caagccttac aggcttcttc
    19261 atattctcca actctttgta cttcccctat aaacaaagta ttgttggctg gataaactgg
    19321 cttatctttt aaattcttaa ctattgtctg tgtaccatct ccacatgcta aagataaaac
    19381 tgcatcagct tccttagtct cttcttttag agcctttaaa tcctttttag actttaaaag
    19441 gttacaagca gggtcaagca ttacatatcc taagattttt ttgccttcac cttcaagagt
    19501 ttccttcatt tttaaaactt cttcttcccc accactttta caagttgctg cacattgatt
    19561 acaaccaact aaaaccagct tgtcaaaatc ttttaagtac cctagcactt cttctaatgg
    19621 tttattttca gaaattatca tatctacttc ccccctaaac cttccacata tatatatcta
    19681 tttattgttt tgtaatttac aagccttaac tacatgtttt gcaagtatag atgttgtaac
    19741 tgaaccaacc cccgctggaa ctggagttat cattgatact ttgtctaaaa ctgcatttgt
    19801 atcaacatct ccacataaat ttccttcttc atcaacattt ataccaacgt ctataactac
    19861 cgctccatct ttaacaaaac tttcatcaac catcttagct ctaccaatag ctgctattag
    19921 cacgtctgcc tctgctgcta cacctgatag atttttagtt tttgaatgac atattgtaac
    19981 agttgcatgt tcacttaata aaagcattga tactggcttt ccaactacca ttgatcttcc
    20041 aagaactgtt accttacttc cttttaaatc aacattatag tgttttaata tctcaactac
    20101 tgctgtaggt gtacatggtg gaaatccact tttatcccct tccattacct tagcagagtt
    20161 tattggacta aaacaatcta cgtctttttc cggtgcaata acatatttta taacttcttc
    20221 atttaagtgt tttggaagag gtctaaaaca taatatacca ttaacatttt tgtcatcatt
    20281 aactctcttt aatacatcaa tatattcctc ttgtgttata tcctcagcta actctaacac
    20341 ttctgttgtt atacctatgt tttgacatct ctttagtgct cctctttcat aagataaatc
    20401 atcacttctt gccccaactc taactattgt tagtttagga tttatacctt ctttaattag
    20461 taaatcgact tcttttatta attcttcact aatcttatca gctacaggtt ttccttttat
    20521 aatttgtcct ttagttgaca ttccttccat acattcacaa ctcccctaca agcaaactta
    20581 aattaattta ttctatatac cacatattaa taacatagta tctactttcg tcatttaaaa
    20641 attattttcc aagagctttt tcaactttgg cataaacttc atcacaaatt tttacaccat
    20701 cttcaactaa cttatctact tctgttttta ccttattaac atattcttta tcttgtatag
    20761 aatttatatt tataactaca tttaattgtc caccaagtat agcagctctt aaacactgaa
    20821 ctcctacacc aacatcactt atagcaagtc ttgaaccttt atcaactaaa tcttcatgta
    20881 acttaatagc ttcatagcaa actcttacta tgtttaatgg tacttcacaa gcaactttta
    20941 atgccttttc cattgtttct gcttttattc ttttttcttc ttctgtttct ttaggaagcc
    21001 cgtaagcttt tgataatggt aagaaacatt ctgcatcatc atctatcatt tttaataaat
    21061 ctttttctaa ctttcctgcc ttacttaata tctcttttac actttcttca tactcagcat
    21121 acttcttctt ccctattgta aggttacata ccatactacc taaagccata cctattgcac
    21181 ctactagtgc agcagctccc ccaccacctg gtactgcttc tttggaagat agtacttcta
    21241 caaaatcaac acaagtttta tctgctattt tcatgataat cctccttgaa aggatgtagg
    21301 aaaatatctc aaattgttcg agatatttta cgaactattt taagatatct cctactcttt
    21361 atttataaat taatttcctt agaataatcc agctattact ccattttcat caacatctat
    21421 tttttctgct gctggaactt ttggtaatcc tggcatcttc attatttctc cagtaagtgc
    21481 aactataaat ccagctcctg ctgaaattgt tgcttgtctt acagttattc taaatcctgt
    21541 tggtctacct aatttagttt ggtcatcagt taaagaatat tgagtcttag ccatacatac
    21601 tggaacttta gtaaatccaa gtttttctaa gttagctatt tccttatcag cttcaggagt
    21661 ataatctaca ccatctgctc cataaatctt agtagctata gcatttatct tgtcttttat
    21721 agataaatca tcttcatagc agaatctaaa gttattttct tcttcatcca ataatcttaa
    21781 aacttcttta gctactgcaa ttccaccttc tccacctttt gcccatactt cagaaagagc
    21841 aacattaaca cctaattcct tacacttgtc ttcaacaagt ttaagttcag cttcagtatc
    21901 aagtgggaat ctgttgattg caacaactgc tggtaatcca tatacttgag ttatgttttc
    21961 aacatgtttt aataagtttg gaagtccttt ttcaagagct tctaggtttt cgttatttaa
    22021 gtctgcttta gcaactccac cattgtattt taatgctctt acagtagcaa ctattataac
    22081 agcatctggt tttaagtttg ccattctaca ttttatatct aagaattttt ctgctccaag
    22141 gtcagcaccg aaaccagcct cagttattac ataatctgca aagtgcatag ccattcttgt
    22201 agctataaca gagttacatc catgagctat atttgcgaat ggtccaccgt gtacaaatga
    22261 tggtgttcct tcaagagttt gaactaggtt tggttttaaa gcatctttta ataatgcagc
    22321 catagcacca ttagctttta attgtctagc tgttacaggt tcaccttcgt agctgtatcc
    22381 aactattatg tttcctaatc tttcttttaa atcagatata tcacttgcca aacagaacgc
    22441 tgccattatt tcagaagcaa cagttatatc gaatccatct tgtctaactg ctccatcacc
    22501 ttttttaccc ataccatcaa cgatatttct aagttgtcta tcgttcatgt ctacacatct
    22561 tctccaagtt attttcttag ggtctattct aagtgcattt ccttggtgaa tatggttatc
    22621 aagcatagca gctaaaagat tgttagcagc tcctatagcg tgaaaatctc ctgtgaagtg
    22681 taagtttata tcttccatag gaacaacttg tgcatatcct ccaccagctg ctccaccttt
    22741 aacaccaaat actggtccta gagatggttc tcttaaagca actaatacat ttttgtctag
    22801 tttagcaaat gcatcagcaa cacctatagt agtagtagtt tttccttcac ctgctggagt
    22861 tgggtttata gctgtagtta gtattaactt agctttttta ccagtctctc tttttagtag
    22921 attgtagtca acttttgctt tgtatttacc ataaagttct acatcatctt cacctagtcc
    22981 taattttttt gcaacctctc taatatcttg aggcttagct tcttgagcta tttcaatgtc
    23041 agatttaaat cccataactg aatctcctcc cacaacctaa atagatttaa aatataattt
    23101 ttgtaaaaat tgtattaata tgtaaaaatt atattttata agataattat acatgtaaaa
    23161 ttacttaaaa actagctact aagctattaa aatattcaaa cttaatagct agttatttat
    23221 ctaattactt acttattacc tatctattgc ctatgtctaa cctttaacca acttaaaatc
    23281 ccaaatcaaa atttctatct attttattta tccttaagtg acattaattt atcttttatt
    23341 gctttaatct gttctttcgt tgtttcacta gaatcatatg gagattgatt agctctatca
    23401 gaatcaataa catcttggtt ataataaata aatcctaaag attctccatc ttctaagttt
    23461 tggattataa attcttcatc ttccttattt ctaattttat ttcctactac aaatacttta
    23521 tttacaccta tatcatgtcc aagtttctta acttttctat aagtctgtag acttctctct
    23581 cctggttcta caactactat aaaagcatct acaccttggg ctgttcctct tcctaagtgt
    23641 tcaattcccg cttccatatc cataaccact acatctttat tttgtagtat taaatgtgag
    23701 cagagtctct tcaaaagaac atgctctgga catacacatc cagtaccacc agaatctacg
    23761 gttcccaatg taagtaatct tactccattg tattctttac agaaattttc agggatatcg
    23821 tctactttag gattcatctt aaacattttt ccaaatgaac ctacagaagc agctgttcta
    23881 tctgaaacta attttttcat ttctgatata ggtactattg attcatatac ttcttttgga
    23941 aaacctagtg ctaaagctaa attagcatca gggtcggcat caacagcaac aactctgtat
    24001 ccgtcttcag caaacatcct agagagcatt gaagaaaaag tcgttttacc tactccacct
    24061 ttacctgtta ttgctatctt cataatccct accacctttt attactattt gctttttagt
    24121 agttaatttt acagttaaat attttttata ctctatattt ttatacacta aaaataaact
    24181 acagctagtc cttacaggaa ccaattaaat ttaccctact agcttttcaa agcagcatgt
    24241 atagttttat tattctttaa tattgtttat attttattta ttcagtatat tcattatatt
    24301 ttgatgttct ttactttaat atttgacttt attcaacttt actcaaccag aaacttctta
    24361 taaaaacgtt tattctataa cttcactatt ttttaagctt cagccttttc tgccattttt
    24421 tcttcaacta atgcttcaaa gtgaacacgt tttttctcta tatcttctat tattgtagct
    24481 gctaactctt ttggatttgt atttacagta aatgtagctc caactttatc ttttaattca
    24541 ctagttaata tatccactgc tcttgaagaa ccagctattg gaggcattat tcctaaataa
    24601 gtgtctatac cagaagctac aacatagcat cctatagcaa ctgctttttc tgacatccac
    24661 tctggagcta tacctacaac tggaaggtca cacatatcca tatctaagta attagctgct
    24721 gctccaacta aatctagtat acggcttata tcaacacaag aacccatatg tagtactggt
    24781 gggattccta ctaatttaca gacagttgca agacctttac ctgcatattt ttcagccgca
    24841 tcagattcca ttaaaccaaa cttagctgct gctgaagctc cacaacctgt agtaactact
    24901 attatgtcgt ttttgattaa ttctttcatt attgttacgt gagcctcatt tgaaactcct
    24961 ttagcattgt tacatccaac tacaccagct gctcctctta atactccaga ttttagacag
    25021 tctgttaatg gcttaactgt tcctgctggg tctatatgag agtttacaac tctgtctaat
    25081 tgatttacta ctgcttctac actataccca actgttgcgc ctgattttaa ttcaggtata
    25141 agtacttttg atttatctct atttttaaag tttaaaatag cttctttaac tattgcttta
    25201 gcatcatcaa gtgcttgttc ttctctaaat tcaatataag ttgaacctgt tatcttagct
    25261 tttggagaag ttgttacaaa tttagtatgg taacaatctg caactctagc taatgcaggg
    25321 aatatacatt gaacgtcaac tattacagct tcaactgctc cagttattat tgcaagttct
    25381 tgttgatgga agtctcctgc tatttttaca ccatgtctca ttgtaatctc attacctgta
    25441 caacacatac ctgctaagtt tattccatca gcaccaactt ctttagctaa agctactaag
    25501 tctggctctt ctgatgctaa aactatcatt tctgataatg ctggctcatg tccatgtaat
    25561 attatgttaa ctttattttc ttctaatact gctaaattcg cctctgttct tcttggcact
    25621 ggagttccaa ataagatatc gcttagacga gtacctatca tagaaccacc ccaaccatca
    25681 gccatagaag ttctaagact catgtgtatt aaactatcta tatcaccagt acatcctata
    25741 tgagtagagt gcattattgt tgcaatttct ctatctatag ctcttggctc tatagcgtat
    25801 tctttccata ttttttgtct tggttctggt gcatttttta taaatcttaa tgtcccaaaa
    25861 ggttttccaa attccattaa tgcaatttct gccacttcat gtgctacatc atagatatct
    25921 ctaccttctg tctctacatc ccattcttta gctaaagtaa ttaacttagc ttcgtctctt
    25981 attttatagt ttccatttgg atcagctaat gctaatgtat gtgctatatc tctagcatgg
    26041 tctgaatggg cagctgtccc accagcaacc attcttgcaa agtttctacc tactatagta
    26101 tgctcatctg ctccacataa cccccttgga gtcttagggc ttattctaca tggtcccatt
    26161 ccacatattc tacagcaaac accttgaaga ccaaaaccac attgtgcctt aaagccttct
    26221 tttctatcat acattgtttc tacgccttct tctctagctt tagctatcat cttctggctg
    26281 tttaaatcaa ttgttaacat tttttcatcc atattcctat cccctttttt tgatttttaa
    26341 aatcccccag gtgactgact tcatgtcata aaataataaa ataaatgctt tctaagttga
    26401 ttatatacta attttcagtg ctttgcttta gatttaaata tttttttaac aaaattagat
    26461 aatacataaa aattttattt tctacactta atttaagttg attttatcgc ttgtttttgg
    26521 ctatttgtta atatctaaac ctactttttt gatacttcag tttaattaaa ttatgttttg
    26581 atttttttaa atcaaatttg aaatttttct agtcattttt atattattgc agattgtttg
    26641 tgtatttatt atctttcact actgtttgga cttattacag atttttactc cctaaactta
    26701 attagtaaaa acttcttaga atagcatttt ttggactaat ttttttgaat ttttttattt
    26761 ttcctacttt aatattaatt atagcttcaa attgaacatt tgtatatatt catttgaagc
    26821 tataattatt ttttatataa aactttttta atacagcatt aatttagaca ttttgactat
    26881 tttttctact ttaattcatt tatttcaagc gatttatcct atttcatatt tttagctaca
    26941 atagccccat atgcaataga atataattta gattcatgtg aatcagctct tgacactaaa
    27001 actataggag cttttgcacc cataattata cctgctgatt tagagtctgc catatatgta
    27061 aaggctttcc ctattccatt accaacctct attgttggaa ccaatatgat atctacatct
    27121 ccagatactt cacttttaaa cccttttatc tcactagctt cttttgaaac agctaaatca
    27181 aaagccagag gaccttctac aattacattt tcaccaaatt ctccttcttt acaagctttt
    27241 gctaacatat ctgcatctac agttgcttgc atcttaggat ttactttctc tttagctgcc
    27301 aaacatgcaa cttttacagt ttccattcct aaagcttttg cagcttttat agaatttttt
    27361 aatatctttg ccttttgttc ataatcagga gctatattca tacctccatc tgtagttata
    27421 agtaatttat ggtatttttc taactcatat accataacat gacttaataa actatcagtt
    27481 ctaagaccat agtctttatt taacacttct tttaatatga cagatgtatc taataatcct
    27541 ttcattacaa agtcggcttc tttacttgat actaatttaa ctgctatttc tgcacactct
    27601 ttgatatcat ccttatccac tattttgata ccacttaaat caaatcctat ttcttttgat
    27661 atttctctta ttttgccttc atttccaacc aatataggag taattatatt tctttctaca
    27721 gcatccttta ttgccattaa aacttcttta tcatgagctg ctgctacaga gagtacaatt
    27781 ttctctcctc ccttaagttg ttctaaaata tcatctaatt tttttatcaa aagtaacccc
    27841 tcccttatat atatttaact aaataatacc atatttgcta taccttttct caatttattt
    27901 tttatgttat aaagaattta tataactaaa agacagttta atattttatt ctgggatttc
    27961 atcaataact cttaaaagct ttgcaggaac accacctaca atagtatttt ctgcaacatt
    28021 ttttgttaca actgctccag cagcgataat tgcattatct ccaatagtaa ctcctggaac
    28081 tacagttaca ttagcaccaa tccatacatt ttttcctatt gtgattggag aaggataagt
    28141 tgtaccacgt ttctctggtg caataccatg gttaagtgta gccaatacca cattatgccc
    28201 aatttgcgta ttatctccaa tctcaatccc tccctggtct tgaaaatggc aactgctatt
    28261 tataaaaaca ttttttccta ttgtgatgtt ttttccacaa tctgtataaa atggtggaaa
    28321 cataaaaaat gattcatcta ccttttttcc aattaactca gaaaataaac tacggacttc
    28381 ttctggttca tgataagaag aatttaactt acttgtgatt ttcattgctt cattactcaa
    28441 cttaaccata aattcatgta tttctgattt actagaaaca ggttttcttt gattgatatg
    28501 tgttaaaaat tcattgatat tcattttata catcctttca atatgtgctt taatttattt
    28561 gtacattcta aataatattt taataaaaat taatttaatt tatttttat
//
LOCUS       DBGJFF010000041        28504 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.041, whole genome shotgun sequence.
ACCESSION   DBGJFF010000041 DBGJFF010000000
VERSION     DBGJFF010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28504
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.041"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(224..2503)
                     /locus_tag="ACOIAF_001818"
     CDS             complement(224..2503)
                     /locus_tag="ACOIAF_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HGZ2668831.1"
                     /translation="MLSDAKNNRYGLSIIAVISILVITISCLIYGSRLNKTLGEETNQ
                     YLSEIANQSVNVLKKQINGDIKLLESISIFIEGEESFEVDNILSILKRQAINSSFKRM
                     GVILPNGTAYTTDGYIEDFSKRDYFLKSMQGEVVITGKLKDVIEDDKNTNSNINVYSV
                     PIYKDNEVKGVIFATHSTKLYEKILSVPTFNGKGYSYIVRSNGDIVLNPSSKNANSTK
                     ENLFSYIGRSDSISKSTLNDMKSNIKNFKSGSLSYNMSGNGYYLSYAPIGINDWYLFS
                     EVPKTAISEKSYAIIKLTLSACVVLIIIFTALTMYILFIQKKSKEKLEAFAFKDNVTG
                     IGNSNKFNLEGGKFLSSHEKRNLVLIYFDIDKFKLVNDRFGYEEGDRVLKGIAEIVKS
                     IFKEQSVFSRISNDNFAIIFEKKNDRESVVEICELIRKKLSMIKTSLGVELNLIPSIG
                     VYFVEDGETNISTCLDKAMIAKTTVKRKYLKVYEIYAENLKETLIEERDIEQEMHDAL
                     KNEQFKVYLQPKIELSTTKIVGAEALVRWQHPKKGLISPGVFIPIFEKNGFITELDMF
                     VFTQVCKNFKRWENESFPTFPISINLSRVHLENPDFISELERITKEYEIEPNLIEIEL
                     TESAIFDNTKILFKIMQTLKSVGFKISMDDFGSGYSSLNMLKDMPIDVLKLDRQFFIT
                     VGNAKKSQIVVSSIVQMAKQLDIKVVSEGVETVEQAEFLRFIGCDMAQGFLFARPMPI
                     EEYEKLILDRYSLNRKETN"
     gene            complement(2774..4228)
                     /locus_tag="ACOIAF_001819"
     CDS             complement(2774..4228)
                     /locus_tag="ACOIAF_001819"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HGZ2668832.1"
                     /translation="MKLNDIIQGLDIINVKGELNIDINNVQYDSRKVTKGTLFICIKG
                     FVSDGHKYIKDAIEKGASAFLVEEDVAIKGCTFIKVKDTRKDMAKVADNFYNHPSQKF
                     NVIGVTGTNGKTSITTILNEILTLNKNKVGLIGTIKIFDGEKDIVSNSTTPESIDLQY
                     HFNNMLDNGCDYCAMEVSSHSLALNRVDETDFKLGIFTNLTPDHLDFHKDLDDYRKAK
                     EKLFFKTTMANIINIDDEGGKKIYENIKGINVPCYTYGVDTKADFMARDIKSDSDGVS
                     YRLITPSYEEVIFIPVPGMFTVYNTLAVIAACYVLGIPKPIYKEGLRLSNGVSGRFET
                     VPNDKGISVIVDYAHTPDALENVLKTTQQFAEGKIISVFGCGGDRDTEKRPLMGSIGQ
                     KYSDLCIITSDNPRTEEPEAIIKDILEGIDKKKENYHVVVDRKQAIAEAISMAKKDDV
                     VIITGKGHETYQIIGKVKHHFDDKEVANECLSKM"
     gene            4392..5261
                     /locus_tag="ACOIAF_001820"
     CDS             4392..5261
                     /locus_tag="ACOIAF_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HGZ2668833.1"
                     /translation="MDNKIGYLNDNFKIFNIRDKKDIQFEYHNHDFNKIIIFINGNVT
                     YFVDGIPYKLKPWDILFISNNEIHKPVIDSNVFYERIAIWISPEFMKKNSDANSDLEK
                     CFKIATENKCNRLRLNIHDIESIKSFIEQINISESNTEFGNEILRNALFIQLMIFLNR
                     IFLKNENMESTEDVFYDETIKKVLNYINSNIEDKLSIDSISSEFFISKYYLMRKFKSH
                     TGTSIHNYIVQKRLILAKSLITNGHLMSEVCNKCGFNDYSSFVRAFKKNYGVSPKNYI
                     NNKNPLDITLLDE"
     gene            complement(5475..7547)
                     /gene="ptsG"
                     /locus_tag="ACOIAF_001821"
     CDS             complement(5475..7547)
                     /gene="ptsG"
                     /locus_tag="ACOIAF_001821"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-specific PTS transporter subunit IIBC"
                     /protein_id="HGZ2668834.1"
                     /translation="MKKVFGVLQKVGKSLMLPVALLPAAGILLGVSNALAGDALLAHF
                     PALANPTFQLIISIMESSGQIIFNNLPLIFAVGVAVGLTEGEGVAALAAIVGFLILNT
                     SMGVMAGVTEDMIDNSMYASIVGIPTIQTGVFGGIIIGIVAAYLYKKFYKVELPQYLG
                     FFAGKRFVPIITSLVAIAIGIILSFIWPPIQEGLLSFSKNVIEANQTLAAFMEGSLER
                     LLIPFGLHHVFHNPFWYQFGEYVNKAGELVMGDQKIFFAQLKDGVNFTAGTFMTGAFP
                     FMMFGLPAAALAMVHEAKPEKKKFVAGIMASAALTSFLTGITEPIEFAFLFVAPVLFA
                     IHCLFSGLSYAVMQLLGVKIGFTFSGGLIDFLLFGVLPNRTPWYLAILVGIGFAVLYY
                     SVFRFMIRKFNLKTPGREDESETNVEVIEMSKNELASEILIALGNKDNLKSLDACITR
                     LRLVVNDINKVDKEKLKSLGAAGVMVIGNNVQAIFGPKSDSIKSDIKDIIEGKTKVES
                     TVTKNVDKNKVKKNMEIANMENALVEKIDSLTNGEILDIEEVPDSVFSSKLMGDGFAI
                     KSSDGLIYSPVDGTIGVIFPTKHAIIIKSKKSGVEILIHLGIETVNLEGNGFEVFVNV
                     GDEVKAGEKLVKMDLEYIEKNGLSTISPVVFTNLEQNQKLSIKKGITTAKEENRVSIL
                     RDGIEKIG"
     gene            complement(7851..8693)
                     /gene="licT"
                     /locus_tag="ACOIAF_001822"
     CDS             complement(7851..8693)
                     /gene="licT"
                     /locus_tag="ACOIAF_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HGZ2668835.1"
                     /translation="MIIQQIYNNNVVLVLDENIKKELILTGCGIGFQKKKGQEIDKSK
                     IERTFVIQDESFLDKISKLASQVDEKFFEISTEIIAYAEENLNTKLYEYIYVALTDHI
                     AFAIKRYHENITIKNDLLQEIKRIHKKEYEIGKWAVDYINKEFDVKFPVDEAGFIAMH
                     IVNSNYKGSSKESLLITKIVKDILNIIRYYYRVEFKEDDINYDRLLTHLKFFAKRLVK
                     KEKINDTNNEIIDIIKVKYEKDYDCAYKIKTHVEKNYDYYVSQDELLYLTLHIKRVIS
                     VLNL"
     gene            complement(8954..10996)
                     /locus_tag="ACOIAF_001823"
     CDS             complement(8954..10996)
                     /locus_tag="ACOIAF_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HGZ2668836.1"
                     /translation="MSILVTLLGLLVCTTISTVFSKKWSNIPLAIYQIVLGIILSILP
                     FKFSFSFNPEIFVICIIAPLLFSEGQNVSRKELLELRKPILLLAFGLVLITVFAGGIF
                     IHFLIPGMPLSVSFALAAVISPTDLVAVKSITQGLNFPKNMMSILEGESLLNDAAGVV
                     AFKVAVLATVTGVFSIEEAGIQFMITAFGGIIVGSILGYIIIKIRLSLHKWNLEEIPM
                     VIVIQIMTPLFVYFVAEEIGVSGILAVVMAGIAHGIEKEHLQNTTTKLRIISDNTWYV
                     LEYVLNGFVFTLLGFLLPSIYTGLSSKNENMALELTFISTLIVLILFVIRFIWVYLWH
                     NSFIKPKKNPLNNFFVGFLGFKDEEVVKESISKCKYSLIVATCGVHGTFTLATALSIP
                     FYLADKDVFPMRDTVLFISSEVILISLVLATVLLPRLLKNNVQESEPLLSDKEAYKLI
                     LKEAILKLSNEKAQEVQPVIHDLNEQLVDSEKGILNTPDNKLISMMIAEADQQGLLAV
                     LKLLEDGKISDRAFKLYRFYITKSRKSVQKSIFKIVKLKISMWIINRKVKKEVKEKIT
                     KIVSENKEIIKEFHHAYALSTKTAISFINKNTDEDNRHEALIAIRFYNRYLNWITRKI
                     QNDDKFEARVEYYRVMAIQSQRDSVQQLVETKQISREVAKELLENILYDEMMMIES"
     gene            complement(11148..12119)
                     /locus_tag="ACOIAF_001824"
     CDS             complement(11148..12119)
                     /locus_tag="ACOIAF_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2668837.1"
                     /translation="MKKEESLLKVEGLKKYFEVKSGLFDKEIKCVKAVDGISFDLKKG
                     ETLAIVGESGCGKSTAGRTILRLIEKTEGKVEFNGVDIYKLEKEELRKLRPKMQMIFQ
                     DPYSSLNPRLNVEQIISEAVIEHNLVPKNEIKDNILNVVESCGLSKYHLNRKPGEFSG
                     GQRQRIGIARALALNPEFIVCDEPVSALDVSIQSQIINLLMDLQDKYKLSYLFISHDL
                     SVVNHIAHRVCVMYLGSIIEIATKGELYLHSQHPYTKALMSAVPIQDPTIKKNRIILK
                     GDIPSPTNPPSGCKFHTRCPYCKEICKKEIPVLKEISINHFVACHLV"
     gene            complement(12120..13097)
                     /locus_tag="ACOIAF_001825"
     CDS             complement(12120..13097)
                     /locus_tag="ACOIAF_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2668838.1"
                     /translation="MSKALIEVKDLKVYFHTDKGIVKSVNEVSFNINEGETIGIVGES
                     GCGKSVTAMSLMKLLPTSKIEGGEIIFRGKDILKMNEDELMGIRGNEISMIFQEPMTS
                     LNPAFTVGSQIIEGIMIHQDLSKEEAKKKVIDMIKLVEIPRAEEIYNSYPHELSGGMR
                     QRIMIAMALSCNPKLLIADEPTTALDVTIQAQILDIMKNIKEKLNTSIMMITHDLGVV
                     AEMCDKVLVMYAGKIIEVAEVVELFKNPKHPYTIGLLKSKPALGKNKDKRLYSIPGQV
                     PNPIGMPDSCYFSDRCEKVCDKCRTQIPPLIELNSGHSIACWLYKKEEI"
     gene            13316..14875
                     /locus_tag="ACOIAF_001826"
     CDS             13316..14875
                     /locus_tag="ACOIAF_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGZ2668839.1"
                     /translation="MKFKKLASLILVSSLMLTFTACASNDKDKSSGSKSGGTVVIPMA
                     SDPDIINGAFANVKEDSLASNIVYAPLYTYEKGNLVNYLAEKVDFKDSKELTIKLKSN
                     LKWHDGKPITAEDVLFTFNTVLDEKQNSPSRQYLLVGEKPVKVEKIDDLTVKITLPTA
                     SESFLYGISKISPIPKHVFEGESNIAKSEKNNNPVGSGAFKFKEWKKGESIVFEKNAD
                     YFGGEPKADSIALKIIPNEASQEAALNNGEISLMKTSAEGYEKAKSNSNLQTYTYSEE
                     RLNYIVFNQNISNMANKEVRQALSYALNRNEMIESAYGKEGSVPAKSILVPEADFYTE
                     EGVEGYDQDTNKAKDLLDKSGVKIDKLKIGYNTGRFGHKNYALVAQQELKKIGIEAEI
                     VPYESKAFFNILFSNSTECDMYVNGYAWGLEPNPYRGMFETGQYCNQTKYSNAEIDAL
                     WEKGFTELNKEKREEIYKQIQQDISKDAPIYTIDYEQNLMAAQKNLKGIKDAKPSPAI
                     LFEDWSKLYVE"
     gene            14981..15940
                     /locus_tag="ACOIAF_001827"
     CDS             14981..15940
                     /locus_tag="ACOIAF_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2668840.1"
                     /translation="MLKLILNRVKVLIPMLILISILSFGLLELAPGDPADAYINPLMS
                     SQDIENIRVNMGLDKPVYIRYLNWLKNTLSGNLGISYINHMPVTEQIMEKMGNTFILM
                     GTSLIFSILVAIPLGIFLAVNKNSITSKVSSVFNYIGVSIPSFWIGMILISIFSVKLN
                     IFPSGGMHTIGNDSIGDLVKHLVLPVITLGLYNTAIFTNYVEASVNEQLKKQYVITAR
                     AKGLSEKVILFKHVLKNSLTSLVTILGMSIQKLVTGAFVTEVVFSWPGMGRLMIDSIF
                     SRDYTVIMAITMLSALFLILGNLVADILYLLIDPKIKSSKGGF"
     gene            15943..16755
                     /locus_tag="ACOIAF_001828"
     CDS             15943..16755
                     /locus_tag="ACOIAF_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2668841.1"
                     /translation="MFTSILKKLKKDKSFAFACITLSIIIILIITSFILNIDSNAIDT
                     TSKLQEPSLKHIFGTDELGRDYFSRALYGGRISLSVGILSMLISVVFGTAVGVISGYI
                     GGKLDQFLMRLVDMLMSIPSFLVLIVLNTYLTPKVSTIILIIGFLSWMEVSRIVRGET
                     LKIKENEYCLAAKATGIKTRNILLRHIVLNLKETIVVAGSLNIANAILTESALSFLGL
                     GIQLPLASWGNMLQDAQRHIFDKLYLAVFPGLLIFLTVMSLTIISKKFSSNT"
     gene            complement(16971..17873)
                     /locus_tag="ACOIAF_001829"
     CDS             complement(16971..17873)
                     /locus_tag="ACOIAF_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HGZ2668842.1"
                     /translation="MDIKQLKYFVEIVKSGFNLSIASKTLHISQPALSQIIKNFEESE
                     DVYLFERYKGRLNGLTPAGERFYVNAEHIINEYKNMMEDLREDSVQFKGKVRIGIPPL
                     ILGIAFSDVVAQLVANNPDIEFDIVEKGAYDLNRMLILQELDYAVLLHPHKIDKSVVT
                     EHVLQEDELTAFLNINHPLAKKDKIDWNDLNEQLLAIFNPTFMIHHKLMQKFTDENVK
                     LKRYIMSGSWDFLLLSTTNSDFITILPSPVHDFFENSKIIEKPFNHPITWKVILCRPK
                     KERYSHVDQHVFKFIIDFFSKKNS"
     gene            18228..19406
                     /locus_tag="ACOIAF_001830"
     CDS             18228..19406
                     /locus_tag="ACOIAF_001830"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HGZ2668843.1"
                     /translation="MKDVVIVSAVRTPIGSFGGVFKNTSAVQLGTIAVKEAISRVGLN
                     LSEIDEVIIGNVLQTGLGQNVARQIAINAGIPNSVPSYTVNKLCGSGLKSVQLAAQSI
                     TSGENDVVIAGGTENMSQAPYIVPTARFGSKMGNITMVDSMLTDGLIDAFNQYHMGIT
                     AENIATKFEFTREMQDKLALESQNKAENAIKNNRFKEEIVPVDVLIRRGKIETIDKDE
                     YPKLGMTFEGLSKLKPAFKKDGTVTAGNASGINDGAAMLILMSQQKADELGIRPLAKI
                     KSYASAGVEPEVMGTGPIPATRKALKKAGLSINDIDLIEANEAFAAQALAVKNELQID
                     SSKLNVNGGAIALGHPIGASGARILVTLIYEMQKRKVETGLATLCIGGGQGISMVVSR
                     "
     gene            19415..20071
                     /locus_tag="ACOIAF_001831"
     CDS             19415..20071
                     /locus_tag="ACOIAF_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HGZ2668844.1"
                     /translation="MNKIVSIDEALSHVKDGDTIMVGGFMANGSPENLIDYLCSKNIK
                     DLTLICNDTGFIDKGVGKMVVNKQFKKIIASHVGLNKETGRQMNNNETDVELVPQGTL
                     AEQIRAAGYGLGGILTPTGIGTLVEEGKQKIVVDSKEYLLEKPIYADVALLFASKVDK
                     AGNLVYKGSMNNFNNLMASAAKITIVEADEIVEIGDIDPNEVNTPGIFVNYIVEGGNL
                     "
     gene            20071..20736
                     /locus_tag="ACOIAF_001832"
     CDS             20071..20736
                     /locus_tag="ACOIAF_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HGZ2668845.1"
                     /translation="MDKLEMQEYIANRVSKELKDGAVVNLGIGLPTKVANYIPDNVNV
                     ILQSENGFLGLGSAEDGNSSDETIVNAGGQPVTILPGGCFFDSATSFGIIRGGHVDIT
                     VLGALQVDKYGNIANYMIPGKMVPGMGGAMDLVTGAKKVIVAMEHTSKGSAKILNNCT
                     LPLTATNAVDLIVTEMGVMEVTSDGILLKEINPAFTLDDVISATEAPLILSDSLNGKI
                     SVV"
     gene            20841..21617
                     /locus_tag="ACOIAF_001833"
     CDS             20841..21617
                     /locus_tag="ACOIAF_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HGZ2668846.1"
                     /translation="MVKDKVVFVTGAASGIGKQIAESFLKNGAKVMFSDINQEALNNT
                     ITELQKKGYDCMSVKCDVTKEEEINHAIDKTVEKYGRLDILINNAGLQHVSMIEDFPT
                     DKFEFMIKIMLTAPFIATKKAFPIMKKQGFGRIINMASINGVIGFAGKAAYNSAKHGL
                     IGLTKVAALEAADSGITVNALCPGYVDTPLVRGQFNDLSKTRNIPLENVLEEVLYPLV
                     PQKRLLDVQEIADYAMFLASDNAKGITGQACLLDGGYTAQ"
     gene            21694..23028
                     /locus_tag="ACOIAF_001834"
     CDS             21694..23028
                     /locus_tag="ACOIAF_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HGZ2668847.1"
                     /translation="MSIIAPIGIIIGIIAIIYFSVKEIHITIAAPIATLIVVILNKMD
                     VVTSMLGADKNNYMGALGNYIMSYFAIFLLGSILAKFMEESGATVSIADFILNKFGSN
                     HPYRVLVAIFIVSFILTYGGISLFVVMFAVIPLARTLFKKLDISWNLIQIPLWLGIAT
                     ITMTIIPGTPAIQNVIPIQYLNTSLTAATIPSIVGSIGCATFGLVYMKYALNKSIRKG
                     ETYATYTSELEEESLDRELPGFFASIFPLLALVIIAITGSTFGSEFMKKNVIYIALIV
                     AILLSAFLFHKFLSSKIQTLSIGASGSIGPIFATSSAVAFGAVIMAAPGFDVFSKLIM
                     SIPGSPLISLTVLTSTMSAITGSSSGALGIVMPNFADYYLSTGLNPELIHRVATIASN
                     ILTIVPQSGVFLTFLSLSKLTHKTGFKETFIVVAVGSLIAEIIVITLGLLMY"
     gene            23524..24354
                     /locus_tag="ACOIAF_001835"
     CDS             23524..24354
                     /locus_tag="ACOIAF_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HGZ2668848.1"
                     /translation="MMRRNIPTKLKTILALGVLTLATASSAVTASASSLENNVNISNK
                     SLVYSNTKYKFSAKPNCSVKPELKPDKDNSCKDKNHNDKNCNNTNKPEDNNNSGSTNK
                     PDNNKPESNKPEDNNNSGSTDKPEDNNNSDSTNKPDDNNNSGSTSESFSAYQKEVVDL
                     VNIERAKAGLNPLTLDSSISNVATKKSQDMIDNNYFSHNSPTYGSPFDMLKKFGISYK
                     TAGENIAMGQKTPKEVVNAWMNSEGHRKNIMNPNFSKIGVGVAQKSGGSIYWTQIFVG
                     "
     gene            complement(24746..28285)
                     /gene="nifJ"
                     /locus_tag="ACOIAF_001836"
     CDS             complement(24746..28285)
                     /gene="nifJ"
                     /locus_tag="ACOIAF_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HGZ2668849.1"
                     /translation="MAKFMKTLDGNTAAAHVAYAFTDVAAIYPITPSSTMAEVVDEWA
                     SQGRKNIFGQTVNVVEMQSEAGASGTFHGSLQGGALTSTFTASQGLLLMIPNMYKVAG
                     ELLPGVFHVSARALASQALSIFGDHQDVMAARQTGCVLLASGSVQEVADIAPVAHLAA
                     IEGRLPFIHFFDGFRTSHEIQKVELLENEDYASLLNFEAVQAFRDNALSPNHPVARGT
                     AQNPDIYFQTREASNKYYQNIVGIVEKYMEKMSNLTGRKHSLFDYYGAEDAKYVLIAM
                     GSVTETIEETIDYLNAKGEKYGLVKVHLYRPFSMKHFLDAMPSTVERICVLDRTKEPG
                     STGEPLYLDVRDVFYGKENAPMIIGGRYGLGSKDTTPSDIKTVFDNLVSEQPKNGFTV
                     GIVDDVTNTSLTPSEPIKIASKGTIRCKFWGLGSDGTVGANKQAIKIIGDHTEKYAQA
                     YFDYDSKKSGGITMSHLRFGDTPIRATYLIDEADYIACHKQSYVYQYDLLKGLKKGGT
                     FVLNTIWDQAGLEEHLPAHMKQYIAKNDIKFYTVNAVKLGQEIGLGNRINMIMQSAFF
                     KLAEIIPEEDAVKYLKDSIVKAYGKKGEKIVNMNYAAVDAGINALVKVEVPASWENAV
                     DADKGEVKEPEFIKNILRPMTAQEGNNLPVSTFNGIEDGTFPCGTAAYEKRGIAVDVP
                     EWIIDNCIQCNQCSFICPHSCIRPVLVTEEELANAPEGFEAKKALGKGLEGLKYRMQL
                     SPLDCTGCGNCADICPAKEKALVMKPIDTQLDTELDNWAFAVNPDEVAPKGDVMPANT
                     VKGSQFRQPLMEFSGACAGCGETAYIKVVTQLYGDRMMIANATGCSSIWGASAPSIPY
                     TCNHEGKGPSWANSLFEDNAEYGFGMYTAVKQIRNKIVDAMTELVSMDICEDAKAVFT
                     EWLDSRNDGEASKVASAKVVELLEKPACDCTDEKAKELVKAIKDRKDYLVKRSQWILG
                     GDGWAYDIGYGGLDHVLASGEDVNVLVFDTEVYSNTGGQASKATPAAAMAKFAASGKK
                     SRKKDLGMMAMTYGNVYVAQVAMGADKNQFIKAVIEAEKHDGPSLIIAYAPCINHGLK
                     EGMGRTVANEAQAVACGYWHLYRFNPEVKAEGKNPFTLDSKEPTASFKDFILAQVRYS
                     AIAKQFPEEADGLFAKAEADAKERYEGYKKLAE"
BASE COUNT         9804 a         4532 c         3732 g        10436 t
ORIGIN      
        1 ttactatcta aagtattttg ctataatccc atatactctt acctcaaaaa cattcaaatg
       61 taaaaaatac tacatattaa taaactttgc ccaataattc ctaaaataat atcctaacaa
      121 tgatttatgt ttttataata gtatattttt acataaaaaa gtgaacattg attaacaaag
      181 ttcacttttt tataaatttt tatgttataa ttcacaattt tatttaattt gtttcttttc
      241 tatttaaact atatctatct aatattaatt tttcatattc ttctattggc attggtctag
      301 caaataaaaa tccttgagcc atatcacaac ctataaacct taaaaactct gcttgctcta
      361 cagtttcaac accttctgaa acaactttta tatcaagttg ctttgccatt tgaacaatac
      421 ttgaaactac aatttgactc ttttttgcat ttcccactgt tataaagaat tgcctatcta
      481 atttcaatac atcaattggc atatctttaa gcatatttaa agaagaataa ccagaaccaa
      541 aatcatccat agaaatttta aatccaacag attttaaagt ctgcattatt ttaaataata
      601 tttttgtatt gtcaaagatt gcactttctg tcaattcaat ttcaattaaa tttggctcaa
      661 tttcatattc ttttgttatt ctttcaagtt cagatataaa atctggattc tcaagatgta
      721 cccttgataa gtttattgat ataggaaatg tagggaaact ttcattttcc caacgtttaa
      781 aatttttaca aacttgagta aatacaaaca tatccaattc tgttataaaa ccattcttct
      841 caaaaatagg aataaataca cctgggctta ttagtccctt tttaggatgt tgccaacgta
      901 caagggcttc agcacctaca attttagtag ttgacaactc gattttaggt tgtaagtaaa
      961 ctttaaattg ttcatttttt aaggcatcat gcatttcttg ttcaatatca cgttcttcta
     1021 tgagagtttc ttttaaattt tcagcataaa tctcataaac ttttaaatat tttcttttta
     1081 ctgtagtttt tgcaatcata gctttatcaa gacaagtaga aatatttgtt tctccatctt
     1141 caacgaaata tacacctatg gatggaatta aattgagttc tactcccaaa cttgttttta
     1201 tcattgacaa ttttttacgt ataagttcac atatctctac aactgactcc ctatcatttt
     1261 tcttttcaaa tattatagca aaattgtcat ttgaaattct tgaaaataca gattgctctt
     1321 taaatatact ttttactatc tctgctatcc cttttaatac tctatctcct tcttcatacc
     1381 caaacctatc atttactagc ttaaacttat ctatatcgaa ataaattaaa actagatttc
     1441 ttttctcatg agaggataaa aactttcctc cctcaagatt aaatttattt gagttgccaa
     1501 tacctgtaac gttatcctta aaagcaaagg cttctaattt ttccttactc tttttctgta
     1561 taaataatat atacattgtt aatgccgtaa atattataat caatactaca catgctgata
     1621 atgtaagttt tataatagca tatgatttct cggatatagc agttttagga acttcagaaa
     1681 aaagatacca atcattgatt cctattgggg cataacttaa ataatatcca tttccagaca
     1741 tattataact taatgaaccc gatttaaagt tttttatatt tgatttcata tcattaagag
     1801 tagattttga tattgaatca cttctgccaa tgtaagaaaa taaattttct ttagtagagt
     1861 tggcgttttt actgcttgga tttaaaacaa tatcaccatt acttctaact atataagaat
     1921 atccttttcc attaaatgta ggtacagaaa gtattttttc atacaacttc gtactatgtg
     1981 tagcaaatat aactccttta acctcattat ctttataaat tggtacagaa tatacattaa
     2041 tattactatt ggtattttta tcatcttcta ttacatcttt taattttcct gttataacca
     2101 cttctccttg cattgatttt aagaaataat ctcttttaga gaagtcttct atataaccat
     2161 ctgtagtgta tgctgtacca tttggtaaaa taactcccat tcttttaaat gaactattaa
     2221 tagcttgtct ttttagtata gaaaggatat tatctacttc aaaagactct tctccttcaa
     2281 taaatattga aattgactct agtaacttta tatctccatt aatttgtttt tttaacacat
     2341 tgacgctttg atttgctatt tctgaaagat attgattcgt ttcttcacct aatgtcttat
     2401 ttaaccttga accataaatc aaacaggaaa ttgtaattac taagatactt atgacagcaa
     2461 ttatggacaa accataccta ttattttttg catcacttaa cattatgctt cctgcctttc
     2521 tcgaatctat gtatgtataa tcattatctt atgacaatta ttgtaaacat tttataataa
     2581 tacttatttt tccatattta ctgtaattat acaaaattat aaactttatt acaacaataa
     2641 attaattaat atagatatta gggtttatat tattgcatta tctattttat aatcatttac
     2701 atataaaaaa atgtaatatc gaagtgaaac ttgatattac atttttgttc ttgtatttta
     2761 tttacttttt atgctacatt ttacttaaac actcatttgc aacttcttta tcatcaaaat
     2821 gatgcttaac tttacctata atttgataag tttcatgtcc cttacctgtt ataattacaa
     2881 catcatcctt ttttgccata cttatagctt ctgctatagc ttgttttcta tcaacaacaa
     2941 catggtaatt ttcttttttc ttgtcaattc cttcaagtat atcttttatt attgcctctg
     3001 gttcttcagt tcttggatta tcagaggtta taatacataa atcagaatat ttttgtccaa
     3061 ttgatcccat caaaggtctc ttttcagtat ctctatctcc accacatcca aatacagaaa
     3121 ttatttttcc ttctgcaaat tgttgagttg tctttagtac attttcaagt gcgtcaggag
     3181 tatgagcata gtctacaatt acacttatac ctttatcatt tggaactgtt tcaaatcttc
     3241 ctgatactcc attagaaagt ctaagacctt ctttatatat tggtttaggt atgccaagta
     3301 catagcaagc agctataact gctaaagtat tatatactgt aaacattcct ggaactggta
     3361 taaatattac ttcttcatat gaaggtgtta ttaatctata agatactcca tccgaatctg
     3421 attttatatc tctagccata aaatcagcct tagtatcaac accataagtg taacaaggta
     3481 catttatacc ttttatattt tcatatatct tttttccacc ttcatcatct atgtttatta
     3541 tatttgccat agtagtctta aaaaataatt tttcttttgc ttttctataa tcatctaaat
     3601 ctttatgaaa atctaaatgg tctggtgtta aatttgtaaa tatgcctagt ttaaagtctg
     3661 tttcgtcaac tctatttaat gccaatgaat gtgatgaaac ttccatcgca cagtagtcac
     3721 atccattatc caacatatta ttaaaatgat attgtaaatc tatactttct ggagttgttg
     3781 aattggaaac aatgtctttt tctccgtcaa aaatttttat agttcctata agtccaactt
     3841 tattcttatt taaagttaat atctcattta gtatagtagt tatactagtt tttccattag
     3901 tacctgtaac tcctataacg ttaaattttt gagatggatg attgtaaaaa ttatcagcta
     3961 cttttgccat atcttttctt gtatctttta cctttataaa tgtacatcct tttattgcta
     4021 catcttcttc aacaagaaat gcacttgctc ccttttctat ggcatctttt atatacttat
     4081 gaccatctga cacaaatcct tttatacata taaataatgt accttttgta acctttcttg
     4141 aatcatattg tacattatta atatctatat ttaattcacc cttgacattg attatatcca
     4201 acccctgtat aatatcattt aatttcataa atgctttcct ccctttaaac tctttctgct
     4261 aaaattacac tacatataat attatattaa gaatttttta ctatgtgaat ggttaaaaat
     4321 gcttgaaaca ttgcaaaaaa tgctctataa ttttctataa tataactgag gaaaggtctt
     4381 ctataaaaac tatggataat aaaatcggat acttaaatga taactttaaa atatttaata
     4441 ttagagataa aaaagatata caatttgaat accataatca tgattttaat aagataatta
     4501 tatttataaa tggaaatgta acttattttg ttgatggtat tccttataaa ttaaaaccct
     4561 gggatatact ttttatcagc aacaatgaaa tccataaacc agttatagat tcaaatgtat
     4621 tttatgaaag aattgcaata tggattagtc cagaatttat gaaaaaaaat agtgatgcta
     4681 atagtgactt agaaaaatgt tttaaaatag ctacagaaaa taaatgtaat agattgcgtt
     4741 taaatataca tgatattgaa tctattaaga gttttattga gcaaataaat atttccgaat
     4801 ccaatactga atttggaaat gaaatattga gaaatgcatt atttatacaa cttatgatat
     4861 tcttaaacag aattttccta aaaaatgaaa atatggaatc aacagaagac gtcttttatg
     4921 atgaaactat taagaaagta ttaaactata taaattcaaa tatagaggat aagctttcaa
     4981 tagatagtat atcgtctgaa ttttttataa gtaagtatta tcttatgaga aaatttaaat
     5041 ctcatactgg aacgtctata cacaattata tagttcaaaa aagattaatt ttggcaaagt
     5101 cactaataac caatggtcac cttatgagtg aagtttgtaa taaatgtgga ttcaacgatt
     5161 attctagttt tgtaagggca tttaaaaaaa actacggtgt atctcctaaa aattatataa
     5221 acaataaaaa ccctttagat attactcttt tagatgaata gatactttat gtaaatttta
     5281 ttttaataaa aacttgaagt attagtttat ttatcttact aatacctcaa gttttttaat
     5341 gttttaaaat atatcaaaat ataatgatgt ctatttatat tacttaaaat atatattaaa
     5401 taaattgcat gttaactgaa cctatttata tatttattaa atctcacata acttttagtt
     5461 atactatcat atagctatcc tattttttca ataccatctc ttaatatact tactctattc
     5521 tcttcttttg ctgtagttat tcctttttta atgcttaatt tctgattttg ttctaaattc
     5581 gtaaatacca caggacttat tgttgataaa ccattttttt caatatattc taagtccatt
     5641 ttaactaatt tttctcctgc ttttacttca tctcctacat tcacaaatac ttcaaatcca
     5701 tttccttcta aatttacagt ttctatccct aaatgtataa gtatttctac tccacttttt
     5761 ttggatttta ttattattgc atgcttagtt ggaaatataa ctccaatagt tccatcaact
     5821 ggtgaataaa tcaatccatc actagattta attgcaaatc catcccccat cagtttactt
     5881 gaaaatacag aatctggtac ttcctctata tctaatatct ctccatttgt taatgaatca
     5941 atcttttcta ctaaagcatt ttccatattg gcaatttcca tatttttttt cactttattt
     6001 ttatcaacat ttttagtaac agtactttca acctttgttt ttccttctat tatatctttt
     6061 atatcagact ttattgaatc cgattttggt ccaaatatag cttgaacatt gttgccaata
     6121 accataacac ctgctgctcc aagtgatttc aatttttctt tatctacttt atttatatcg
     6181 tttacaacta atctaagtct tgttatacat gcatctagac tctttaagtt gtctttattt
     6241 cctaaagcaa tcaaaatctc tgatgctaat tcattcttag acatttcaat gacttcaaca
     6301 ttagtttctg actcatcttc tcttcctggt gtctttaaat taaattttct tatcataaat
     6361 ctaaatacgc tatagtataa tactgcaaaa ccaataccta ccaaaatagc caagtaccat
     6421 ggagttctat taggaagaac accaaataat aagaaatcta ttaatcctcc tgaaaatgtg
     6481 aatccaattt taactcctaa taattgcata actgcgtatg ataaacctga aaataaacaa
     6541 tgtattgcaa acaatactgg tgcaacaaat aaaaacgcaa actctattgg ctcggttatt
     6601 cctgttaaaa aagatgttaa tgctgctgat gccattatac ctgctacaaa ttttttcttt
     6661 tctggctttg cctcatgcac catagcaaga gctgctgctg gaaggccaaa catcataaat
     6721 ggaaatgcac ctgtcataaa agtacctgct gtgaaattta ctccatcttt aagttgagca
     6781 aagaatatct tttggtcacc cattacaagc tcaccagctt tatttacata ttctccaaat
     6841 tgataccaaa atggattatg gaaaacatga tgcaaaccaa atggtattaa tagtctttcc
     6901 aaagaacctt ccataaatgc tgctagtgtt tgatttgctt ctataacatt ttttgagaaa
     6961 ctaagtaatc cctcttgtat tggaggccaa ataaaagaca atataattcc tattgctata
     7021 gcaactaatg aagtaatgat tggtacaaat cttttacctg caaaaaaacc tagatactga
     7081 ggtaattcaa ctttataaaa ctttttataa agataagcag ccactatccc tataataata
     7141 ccaccaaata cccctgtttg aattgttggt attccaacaa tacttgcata catagaatta
     7201 tctatcatat cttctgtaac acctgccatg acacccatag atgtattgag aattaaaaaa
     7261 cctactatgg ctgccaatgc tgcaacacct tctccttcag ttaaaccaac agctacccca
     7321 actgcaaata taagtggtaa gttattaaat ataatttgac ctgaactttc cattatactt
     7381 attataagtt gaaatgtagg attcgccaaa gctggaaaat gagccaacaa tgcatctcct
     7441 gctaaagcat tactcacgcc aagtaatata cctgctgcag gaagtaatgc aactggtagc
     7501 attagagatt ttcctacttt ttgtaaaaca ccaaatactt ttttcattta cttttctctc
     7561 cttattattt tatatttttt taaataaaaa aagacttaag tttataaaaa gttacattag
     7621 tatagttaat tctaagatta aacttatgtt aatataaata aacctatatt aacctaattt
     7681 cagaattaaa aataaactat tataattttt ttataaacat aagtcttgcc tgcattatca
     7741 gtaacaatct taatgtttta aaattattat tttatattaa attttaaata atatattaaa
     7801 attaaacata atatattaaa tctatacatt ctgtattaaa ctactcttta ttataaattc
     7861 agtacagata taactctttt gatatgtaat gttaaataaa gtagttcatc ttgacttaca
     7921 tagtaatcat aattcttttc tacatgagtt tttattttat atgcacaatc ataatccttt
     7981 tcatacttta ctttaattat atctataatt tcattatttg tatcattgat tttttctttt
     8041 tttactaatc gttttgcaaa gaattttaaa tgagtcagaa gcctatcata atttatatca
     8101 tcttctttaa attcaactct ataataatat cttataatat ttagtatgtc ttttacaatt
     8161 ttagtaatta acagtgattc ctttgaactt cccttatagt ttgaatttac tatatgcatt
     8221 gctataaaac cagcttcatc tactggaaat ttcacatcaa attctttatt tatataatct
     8281 acagcccatt tcccaatctc atattctttt ttatgtattc tttttatctc ctgtagtaaa
     8341 tcattcttta tagttatatt ttcatgatat cttttaattg caaatgcaat atggtcagta
     8401 agtgctacat aaatatattc ataaagctta gtattcaaat tctcctctgc ataagcaatt
     8461 atttcagtac taatctcaaa aaatttctca tcaacctggc ttgctagttt actaatttta
     8521 tctaaaaagc tttcatcttg tattacaaaa gttctttcaa ttttactttt atctatttct
     8581 tgacctttct ttttttgaaa cccaattcca catcctgtta atattaattc tttttttata
     8641 ttttcatcta aaactaatac aacattatta ttataaattt gttgaataat cataatcaat
     8701 atctctccca aaatacaatt tcccttattc ataaaaaaat acctaaatta agatacaatt
     8761 cacctgtata ccttaaccta ggtattgcct gcttaccagt aacaatccag cttgttaacg
     8821 cttatatatt gtattcaatt tatggtttta ttctattata aataaatata tatgtcaagt
     8881 aaattattga aaactatttc ctatttttta gtattaattt taacatcaaa aatcaaattt
     8941 tattccatta tatttatgat tctatcatca tcatttcatc atagagaata ttttccaata
     9001 actctttagc cacttctctt gatatttgct tagtttcaac taattgttga acactgtctc
     9061 tttgactttg tatcgccatt actctgtagt attctactct tgcctcaaac ttatcatcat
     9121 tttgaatctt tctagtaatc caattcaaat aacgattata gaatctgatt gctattaagg
     9181 cctcatgtct gttatcttca tcagtatttt tatttataaa tgatatagca gttttagtag
     9241 aaagtgcata tgcatggtga aactctttga ttatttcttt attctcagat actatttttg
     9301 taattttttc ttttacttct ttctttactt ttctatttat aatccacatg cttattttta
     9361 attttacaat tttaaatata gatttttgaa cagattttct tgattttgta atataaaatc
     9421 tatacaattt aaaggctcta tcagaaattt taccatcttc taatagtttc aaaacagcta
     9481 ataatccctg ttggtctgct tctgcaatca tcatacttat aagtttatta tctggagtat
     9541 ttagtattcc tttttctgag tctaccaact gttcatttaa atcatgtata actggttgta
     9601 cttcttgtgc tttttcatta ctaagtttta aaatggcttc tttcaaaatt agtttatatg
     9661 cctctttatc agataataaa ggttctgact cttgtacgtt attttttaat aatcttggca
     9721 gtaaaactgt tgccaatact aaacttataa gaataacttc tgatgatata aataaaacag
     9781 tatctctcat tggaaataca tctttatcag ccaaataaaa aggtatagat aaggcagttg
     9841 ctagtgtaaa tgtaccatga acaccacaag tagcaactat tagtgagtat ttacatttag
     9901 aaatactctc ttttacaact tcttcatcct taaaacctaa aaaacctaca aaaaaattat
     9961 ttaaaggatt ctttttaggt tttataaaag aattatgcca taaatatacc catataaatc
    10021 ttataacaaa taaaataagt acaattaaag tagaaataaa agtcaattcc aatgccatat
    10081 tttcattttt actactaagt cctgtatata tacttggtaa caaaaaacca agcagtgtaa
    10141 agacaaatcc attcaataca tactccaaaa cataccaagt attgtctgat attatcctta
    10201 atttagtagt tgtattttgt aaatgctctt tttcaattcc atgagctatc cctgccatta
    10261 caacagctag gattccagat actcctattt cttctgctac aaaatataca aaaagtggag
    10321 tcattatttg tataactatt accattggaa tttcttccaa attccacttg tgtaaactaa
    10381 gtcttatttt tataattatg tatcctaaaa tacttcctac tattattcct ccaaaagctg
    10441 taatcataaa ttgaatccct gcctcttcta ttgaaaaaac tcctgtaact gtggcaagta
    10501 ctgcaacctt aaatgctact actccagcag catcatttaa taaagattca ccttcaagta
    10561 tactcatcat atttttagga aaatttaatc cttgtgtaat tgattttact gctactaaat
    10621 cagttggaga tattactgct gccaaagcaa atgaaacaga taatggcatc ccaggaataa
    10681 gaaaatgtat aaagattcca cctgcaaaca cggttattaa aactaaacca aatgcaagaa
    10741 gcaaaattgg ttttctgagc tctaatagct cttttctcga tacattttgt ccttcactga
    10801 atagtaatgg agctatgata catattacaa atatttctgg attaaatgaa aatgagaatt
    10861 taaatggcaa aatagacaat ataatcccta gtacaatttg atatattgca agaggaatat
    10921 tactccactt tttgctaaat acagttgaaa tcgttgtaca aaccaataat cccaacaatg
    10981 ttactaaaat tgacataact acctctcttt tcaataataa tattatagat taaacttata
    11041 tatcaactaa cttaatatta acagcatgaa atcccatctt aatcaaaaaa attttcagtt
    11101 tattctatta ttcttattat atacatcaaa tttaattttt gaaatatcta tactagatga
    11161 catgcaacaa aatgatttat tgatatttct tttaaaactg gaatctcttt tttgcatatt
    11221 tccttacaat aagggcatct tgtatgaaac ttacatccac ttggtggatt tgttggagat
    11281 ggtatgtctc cttttaatat aattctattt ttcttaattg tagggtcttg tattggtact
    11341 gcgctcataa gcgcttttgt atatggatgt tgagaatgta agtaaagttc tcctttagtt
    11401 gcaatttcta ttatagaacc tagatacata acacaaactc tatgtgcaat atgatttact
    11461 acactcaaat catgagatat aaataaatat gaaagtttat atttatcctg taaatccata
    11521 agtaaattta taatttgaga ttgaatagaa acatctagtg cagaaacagg ttcatcacaa
    11581 actataaatt ctggatttaa tgctaatgct cttgctatac ctattctctg tctttgacct
    11641 ccagaaaact cacctggttt tctatttaaa tggtacttag aaagaccaca actttctaca
    11701 acatttaata tattatcttt tatttcattc tttggcacta aattatgttc tataactgcc
    11761 tcggatatta tttgttccac atttaatctt ggatttaatg aactatatgg gtcttgaaat
    11821 atcatttgca ttttgggtct taactttcta agttcttcct tctctaactt atatatatct
    11881 acaccattaa attcaacttt accttcagtt ttttcaatta atctaagaat ggttcttcct
    11941 gcagttgatt ttccacatcc cgattctcca actatagcca aagtttctcc tttttttaaa
    12001 tcaaaagaaa ttccatctac tgcctttaca catttaattt ccttatcaaa taatccagat
    12061 ttaacttcaa agtacttttt aagaccttct acttttaata aactctcttc ttttttcaat
    12121 tatatctcct cctttttata taaccaacat gcaatactat gaccactatt caattctatt
    12181 aaaggaggta tctgagttct acatttatca catacttttt cacatctatc actaaaataa
    12241 catgaatctg gcattccaat tggatttggt acttgtcctg gaatcgaata caatctttta
    12301 tctttgtttt ttccaagagc aggctttgat tttagaagtc caatagtata aggatgtttt
    12361 ggatttttaa ataattcaac aacctctgcc acttctataa ttttaccagc atacatcact
    12421 aatactttgt cacacatttc tgcaactact cccaaatcat gtgtaatcat cattatagat
    12481 gtgtttaatt tttcttttat atttttcatt atatctaaaa tctgagcttg aatagtaaca
    12541 tctaaagctg ttgttggttc atcagctata agtaattttg gattacatga taaagccata
    12601 gctatcatta ttcgttgtct cataccacct gataattcat gtggatatga attatatatt
    12661 tcttcagctc ttggtatttc taccagtttt atcatatcga ttaccttttt cttagcttct
    12721 tcctttgata aatcttgatg tatcataata ccttcaatga tttgacttcc aacagtaaac
    12781 gctgggttta aagatgtcat tggttcttga aaaatcattg atatctcatt ccctcttatt
    12841 cccataagtt catcttcatt catctttaaa atgtctttgc ctcgaaagat tatttctcca
    12901 ccttctattt ttgatgttgg aagtaacttc atcaaagaca tggctgttac agacttacca
    12961 catcctgatt ctccaactat tccaatagtt tcaccttcat ttatattaaa acttacttca
    13021 tttacactct ttacaatccc tttatctgta tgaaaataaa cttttaaatc tttaacctca
    13081 attagtgctt tactcattat tttctcctct cagcaaaatt acttaagata acatacacct
    13141 tttgattgta ttttataaaa atttaaatta caacattttc ttacatttta ttattttttt
    13201 ctattaatta taataatata atagttaatt tctatatata ttgatttaat aatgattact
    13261 attatacaat aaaaaatatg tgttaaatat tattttatgg aggttttata agaatatgaa
    13321 atttaaaaaa ttggcatcat taatcttagt ttcatcatta atgcttacat ttacagcctg
    13381 tgcttccaat gataaagaca agtctagtgg aagcaaatct ggtggaactg ttgtaatacc
    13441 tatggcaagt gacccagata taatcaatgg agcttttgca aacgttaaag aagactctct
    13501 tgcatcaaat atagtttatg ctcctcttta tacttatgaa aaaggaaatt tagtaaatta
    13561 tttagcagaa aaagtagatt ttaaagatag taaggaatta actataaaat taaaatccaa
    13621 tttaaaatgg catgatggaa agccaataac tgctgaagat gtcttattta catttaatac
    13681 agttttagac gaaaaacaaa attcaccatc aagacaatac ttattggtgg gtgagaaacc
    13741 agttaaagta gaaaaaattg atgatttaac agttaagatt actttaccaa ctgcaagtga
    13801 atctttctta tatggtatat ctaaaatttc tccaatacca aaacatgtgt ttgaaggaga
    13861 atcaaatata gctaagtcag aaaaaaataa taatccagtt ggttctggtg cattcaagtt
    13921 taaggaatgg aaaaaaggtg aaagcatagt atttgaaaag aatgcagatt attttggtgg
    13981 agaacctaag gcagattcta tagcgctaaa aataattcct aatgaagcat ctcaagaagc
    14041 agctttaaat aatggcgaaa tttcacttat gaagacatct gctgaaggat atgaaaaggc
    14101 aaaatcaaac tcaaacttac aaacttatac ttatagtgaa gaaagattaa actacattgt
    14161 atttaaccaa aatatatcaa acatggctaa caaagaagta agacaagcct tatcatatgc
    14221 cttaaataga aatgaaatga tagaatcagc atatggaaaa gaaggttctg tacctgctaa
    14281 atctatatta gttccagaag cagacttcta tacagaagaa ggtgttgaag gatatgacca
    14341 agatacaaat aaggctaaag acttattgga taaaagtggt gttaaaattg ataaattaaa
    14401 aataggttat aacacaggta gatttgggca taaaaactat gccttagtag ctcaacaaga
    14461 gttgaaaaaa attggtatag aagctgaaat agttccttat gaaagtaagg cattcttcaa
    14521 tatactattt agtaatagca ctgaatgtga tatgtatgta aatggttatg cttggggatt
    14581 agaaccaaac ccatatagag gtatgtttga aactggtcaa tattgcaacc aaactaaata
    14641 ttctaatgct gaaatagatg ctttatggga aaaaggattt actgagttaa acaaggaaaa
    14701 acgtgaagaa atatataagc aaatacaaca ggatatatct aaggatgcgc ctatttacac
    14761 tatagattat gaacaaaatt taatggcagc tcaaaaaaat ctaaaaggaa taaaagatgc
    14821 taaaccatct cctgcaatat tatttgaaga ttggtctaaa ctgtatgtag aataattgat
    14881 gtaaaatttt atattattaa ttagaaaagg agatgtctaa aaataatatt gttttattat
    14941 tttaaaacga gacatctctt ttaattagga gaatttactt atgttaaaat taatattgaa
    15001 tagagtaaaa gttttaatac ctatgttaat cttaatatct atattatcat ttggactttt
    15061 agaattagct cctggtgacc cagcagatgc atatataaat cctttaatgt cttcacagga
    15121 tattgaaaat ataagggtta atatggggct tgataaacca gtatatataa gatatttaaa
    15181 ctggttgaaa aacactttaa gtggaaactt aggtatttct tatataaacc atatgcccgt
    15241 tacagaacaa attatggaaa aaatgggaaa tacatttatt cttatgggta cttctttgat
    15301 attttcaata ttggtagcta tacctcttgg aatattttta gctgtaaata aaaattcaat
    15361 aactagtaaa gtatcatctg tttttaatta tataggtgtt tctatacctt ctttttggat
    15421 aggtatgata ctcatttcaa ttttttcagt taaacttaac attttcccat caggaggtat
    15481 gcatactatt ggaaatgata gtattggaga tttagtaaaa catttagttt tgcctgtaat
    15541 aactttaggt ctatataata ctgctatttt tacaaattat gtagaagcta gtgtaaatga
    15601 acaattaaaa aaacaatatg ttattacagc aagagcaaaa ggattatcag aaaaagtcat
    15661 actatttaaa catgtcttga aaaattcact gacatcattg gttacaatcc taggcatgag
    15721 tatacaaaaa ttagtcactg gtgcatttgt tacagaagta gttttctctt ggccaggtat
    15781 gggaagatta atgattgatt caattttttc aagagattat actgtaatta tggcaataac
    15841 tatgttatca gcactatttt taattttagg taatttagtt gctgatatac tatacttatt
    15901 aattgaccct aaaataaaat ctagcaaagg aggattctag ttgtgtttac aagtatctta
    15961 aaaaaattaa aaaaagataa atcctttgct tttgcatgta ttactcttag cataataatc
    16021 atattaatta taacttcttt tatattaaat atagactcta atgcaattga tactacttct
    16081 aaacttcaag aacctagctt aaagcatatc tttggtacag acgaacttgg tagagattac
    16141 ttctctagag ctctttatgg tggaaggatt tcacttagtg ttggtatttt atcaatgctt
    16201 atttctgtag tatttggtac tgctgtaggt gttattagtg gatatattgg aggtaagcta
    16261 gaccaattct tgatgagatt agtggatatg cttatgagta taccaagttt cttagttttg
    16321 attgtattaa atacttatct aacacctaaa gtttccacta ttatactaat tattggattc
    16381 ttaagttgga tggaagtttc aagaattgta cgtggcgaaa cattaaaaat aaaggaaaat
    16441 gaatactgtc tagctgctaa agctactggt atcaaaacta gaaatatact attaagacat
    16501 atagttttaa acttaaaaga gactattgta gttgctggtt cgttaaatat tgctaatgct
    16561 atcttaactg aatctgctct tagtttttta ggtcttggaa tccaattgcc tcttgcatca
    16621 tggggtaata tgcttcaaga tgctcaaaga catatttttg acaagttata tttagctgta
    16681 tttccaggat tacttatatt tttaacagtc atgagtttga ctataataag caaaaaattt
    16741 tctagtaata cttaacaaat tttatataga aaataaaaac agtgattatc taaagatata
    16801 cactgttttt atttttcgtt taaaaaaata catattttgt atattataac tttaaatact
    16861 actctaccat gttatgtaaa gcctattatt gctctcaaac atatagatga aaaactaata
    16921 gtttattata ataaaaattg atttatttat ttgttatttt aataacattt ttaagagttc
    16981 tttttactaa agaaatctat aataaattta aatacatgtt ggtctacatg gctataacgt
    17041 tcttttttag gtctacataa tataactttc catgtaatag gatgattaaa aggcttttct
    17101 ataattttgc tattttcaaa aaaatcatga actggtgatg gtagaattgt tataaaatca
    17161 gaatttgtgg ttgatagcaa caaaaaatcc catgaaccag acattatata tctttttaac
    17221 ttaacatttt catcagtaaa tttttgcatt agcttatgat gtatcataaa agtaggatta
    17281 aatattgcaa gtaactgctc attcaaatcg ttccaatcta tcttatcctt cttagcaagt
    17341 ggatgattta tatttaaaaa tgctgtaagt tcatcttctt gaagcacatg ttcagttact
    17401 acacttttat caattttatg tggatgtaac aatactgcat aatctaattc ctgtaagata
    17461 agcattctat ttaagtcata tgcacccttc tctactatat caaattctat atctggatta
    17521 tttgcaacta actgtgcaac tacatctgaa aatgctattc caagtataag tggaggtatt
    17581 cctattctaa cttttccttt aaattgaact gaatcttctc ttaaatcttc catcatattt
    17641 ttgtattcat ttatgatatg ttctgcattt acataaaatc tttctcctgc tggtgttaac
    17701 ccatttaacc tacctttata cctttcaaat aaatatacat cttcactttc ttcaaaattt
    17761 tttataattt gacttaatgc tggttgagat atgtgaagtg tcttagatgc tattgataaa
    17821 ttaaaaccag atttcactat ttcaacaaaa tatttcaact gttttatatc cattttaaac
    17881 ccccttcccc aaaagtataa tttagattat attttttctt ctaatgatat tttataggaa
    17941 aatcaacata atttcattaa tattttaaca aaatattttt ctaaatctac tataaatttt
    18001 tatcctataa atttttctta tacactatat aaaaaattag acttagacaa aatgcttttt
    18061 tcgccgtaat ctataattag aaacaggaaa acctaaagca ttaggaataa aagtaattga
    18121 aatacttaaa aacaagcttg tattaaaaaa gtactttatt atagtgttcc ctaatttctt
    18181 tattcagaaa atttttaatt atcattcaag aaaggtaact attagatatg aaagatgtag
    18241 taatcgtatc agcagtcaga acacctatag gaagttttgg aggtgtattt aaaaatactt
    18301 cagctgttca acttggaact atcgctgtta aagaagcgat atcacgtgta ggacttaatt
    18361 tatctgaaat agatgaagta ataattggaa atgttcttca aacagggctt ggacagaatg
    18421 ttgctagaca aattgctata aatgcaggta taccaaattc tgttccaagc tacacagtaa
    18481 ataaattatg tggttcaggg cttaaaagtg tccagctagc tgcccaatca atcacatctg
    18541 gtgaaaatga tgtggtaatt gcaggtggta ctgaaaatat gagtcaagct ccttatatcg
    18601 ttccaactgc tcgctttggt agtaaaatgg gtaacataac catggtagat agtatgctaa
    18661 cagatggcct tattgatgct ttcaatcagt atcatatggg aataactgca gaaaatattg
    18721 caactaaatt tgaatttact agagaaatgc aagataaatt ggcattagaa agtcaaaata
    18781 aggctgagaa tgctataaaa aacaaccgct ttaaagaaga aatcgttcca gtagatgttt
    18841 taatacgtcg aggaaaaata gaaactattg ataaagatga atatcctaaa ttaggtatga
    18901 catttgaagg tttatctaaa cttaaacctg cattcaaaaa agatggtact gtaacagctg
    18961 gaaatgcctc aggtattaat gatggtgcgg ctatgttaat actaatgagc caacaaaaag
    19021 cagacgaact aggtattaga ccacttgcta aaataaaatc ctatgcttca gctggtgttg
    19081 aacctgaagt catgggtact ggtccaatcc ctgcaactag aaaagcactg aaaaaagctg
    19141 gacttagcat caatgatata gacttaatag aagcaaatga agcgtttgct gcccaagcat
    19201 tagctgttaa aaatgaactt caaatagatt catcaaagtt aaatgttaat ggtggtgcaa
    19261 tagcattagg acatccaata ggagcaagtg gagctagaat acttgttacc ttaatttatg
    19321 aaatgcaaaa acgtaaagtt gaaactggac ttgcaacact ttgtattggt ggtggtcaag
    19381 gaatatcaat ggtggtttca cgataggagg aaatatgaat aaaatagtta gcattgatga
    19441 agctctatct catgtaaaag atggagatac aataatggtt ggtggattca tggccaatgg
    19501 ttcaccagaa aatttaattg attatttatg tagtaaaaat ataaaagatt tgacactaat
    19561 ttgcaatgat actggcttta ttgacaaagg agttggcaaa atggttgtaa ataagcaatt
    19621 taaaaagata attgcttctc atgtaggatt aaataaagaa actggaagac aaatgaataa
    19681 caatgaaaca gatgttgagt tagttcctca gggaactctt gctgaacaaa tacgtgcagc
    19741 tggttatggt ctaggaggta tactcacacc gactggtatt ggtactcttg ttgaggaggg
    19801 aaagcaaaaa attgttgtcg atagcaaaga ataccttctt gaaaaaccaa tttatgcaga
    19861 tgttgctctt ctatttgcat caaaggtcga taaagcaggt aatctagttt ataaaggctc
    19921 tatgaacaat tttaataatt taatggcatc tgctgccaaa attacaatag ttgaagcaga
    19981 tgaaatcgtt gaaattggtg atattgaccc aaatgaagtt aatacaccag gtatttttgt
    20041 gaattacatc gttgaaggag gtaatttata atggataaat tagaaatgca agaatatatt
    20101 gcaaatagag tatctaaaga attaaaagac ggagccgttg taaaccttgg aataggtcta
    20161 ccaaccaaag tagcaaatta tattccggat aatgtaaacg ttatcttaca atctgaaaat
    20221 ggctttttag gtttaggttc agccgaagat ggaaattcaa gtgatgaaac tattgtaaat
    20281 gcaggtggtc aaccagttac tatacttcct ggaggatgtt tctttgatag tgctacttct
    20341 tttggaataa tcagaggagg tcatgttgat ataactgttc ttggcgcact tcaggttgac
    20401 aaatacggca atatagcaaa ctatatgatt ccaggtaaga tggttccagg tatgggtgga
    20461 gctatggacc tagtaactgg agctaaaaaa gttattgtag caatggaaca tacttcaaaa
    20521 ggttctgcaa aaatattaaa taattgtact cttccactta ctgcaacaaa tgctgtagat
    20581 ttgatagtta ctgaaatggg agttatggaa gtaacatcag atggaatttt acttaaagaa
    20641 ataaatccag ctttcacatt agatgatgtt atttctgcta ctgaagctcc tttaatattg
    20701 tcagattcat taaatggaaa aatatctgtt gtttaaaata attaaaacta gaaaataaaa
    20761 atctcttaga cgtattacat ataaatttgt attgcttaaa atattaatat aaaaataaaa
    20821 taaaattgga ggaaatgaaa atggttaaag ataaagttgt attcgtaaca ggagctgcaa
    20881 gtggaatagg taaacaaata gctgaatcgt tcttaaaaaa tggtgctaaa gtaatgttct
    20941 ctgatataaa tcaagaagct ttaaataata ccataacaga gttacaaaaa aaaggttatg
    21001 attgcatgag tgtaaagtgt gatgtaacta aggaagaaga aattaaccac gctatagaca
    21061 aaactgttga aaagtatggc agattagata tattaataaa caatgctgga cttcaacatg
    21121 tttctatgat agaagatttc ccaactgata aatttgaatt tatgataaaa ataatgctga
    21181 cagcaccttt tatagctact aaaaaagcat ttcctataat gaaaaaacaa ggctttggtc
    21241 gtataataaa tatggcctca atcaatggtg taataggatt tgctggaaag gctgcttata
    21301 actcagcaaa acatggactt attggactaa caaaagttgc tgctcttgaa gctgccgatt
    21361 ctggtataac agttaatgct ttatgccctg gatatgtaga tactccactt gtcagaggcc
    21421 agttcaatga cctttcaaaa actcgtaata taccattaga aaatgtctta gaggaagttt
    21481 tatacccact tgtaccacaa aaacgcctat tagatgttca agaaattgcc gactatgcta
    21541 tgttcttagc aagtgataat gctaagggta ttacaggaca agcttgctta ctagatggag
    21601 gatatactgc acaatagtta ttatcacaat agttattata tttagggctt gaagaaagta
    21661 ttcttcaggt ccttctacta aggagggata tttatgagta ttatcgcgcc aattggtata
    21721 attattggta tcatagctat tatatatttt tcagttaaag aaattcatat aactatagct
    21781 gctccaatag caactttaat agttgttatt ttaaataaaa tggatgttgt aacatcaatg
    21841 ctaggtgctg acaaaaacaa ttatatgggt gcacttggaa actatataat gagttatttt
    21901 gcaatattct tattaggctc aatacttgct aaatttatgg aagaaagtgg agctactgta
    21961 tcaatagctg attttatttt aaataaattt ggctctaatc atccttatag agttttagtt
    22021 gctatattta tagtaagttt tatattaacg tatggtggta taagcttatt tgtagttatg
    22081 tttgcggtca ttccacttgc aagaactcta tttaaaaaac ttgatatttc ttggaacctt
    22141 attcagatac cactttggct tggtattgca acaattacta tgactataat tcctggaaca
    22201 ccagccattc aaaatgttat acctattcaa tatctaaata cgtctcttac tgcagctact
    22261 ataccaagta ttgtaggtag tataggttgt gctacgtttg gacttgtcta tatgaaatat
    22321 gctctaaaca aaagtattag aaaaggtgaa acttatgcaa cttatacttc agaattagaa
    22381 gaagaatctt tagatagaga attaccagga ttttttgcaa gtatttttcc tctattagca
    22441 ctcgtaatta tagctataac tggaagtact tttggttcag aatttatgaa aaaaaatgta
    22501 atttacatag ctttaatagt tgcaattctt ttatctgctt ttttattcca taaatttcta
    22561 tcatctaaga tacagacatt gagcataggt gctagtggtt ctataggtcc tatatttgct
    22621 acatcttctg cagttgcatt tggagcagta ataatggctg cacctggttt tgatgtattt
    22681 agcaaactta taatgtcaat accaggtagt ccacttataa gtcttactgt tttgacttca
    22741 acaatgtcag caataacagg gtcctcttct ggtgcattag gaatagtaat gccaaacttt
    22801 gcagattatt atttaagtac tggtctaaat ccagaattaa ttcaccgtgt tgctacaata
    22861 gcttcaaata tactaactat agttccacaa agtggtgttt ttcttacatt cttaagctta
    22921 tctaagttaa cacataaaac tggatttaaa gaaactttca ttgtagttgc agttggttca
    22981 ctaatagcag aaattattgt aattacatta ggattactaa tgtattaata taaaacaaat
    23041 taaaattcat aatatgaaag gtggtgtctc aaagtgaact ttttacttaa tgagacactc
    23101 tttttatatg aaataacata tgcctatcat ttaaacaata aaaaaggcct tatctctatt
    23161 ttgagacaga ccctttttat ttcttaaaga tttaaataat tataatttgt tgctaatacg
    23221 aaagcatcta ctgaataaat ttaaccaaat aaaaaaaaat ttatcataac tataaattta
    23281 cgttatggaa ttattataaa aatattattt tctctaacta agtaattcca tactatacag
    23341 gcttttaata caaattgtca aacacactga aatataccta tttattccat atatatcttt
    23401 tttacatatc tttacagtaa aaaaagtctt aatcttatgt tagtatagat tcacgggtaa
    23461 ccccctacac taaaggttac aaatcagtaa catttttaga atttttagat taagaggtgt
    23521 ttaatgatga gaagaaatat accaacaaaa ttaaaaacta tattagcttt aggagtgtta
    23581 actttagcaa cagcttcaag tgctgttact gcaagtgctt catcacttga aaataatgta
    23641 aatataagta acaaaagttt agtatatagt aatactaaat acaagttttc tgcaaaacca
    23701 aattgttctg taaaaccaga acttaagcct gataaagata actcttgtaa ggataagaat
    23761 cataatgaca agaattgcaa caacactaat aaacctgaag acaacaataa ttcaggctct
    23821 actaataaac ctgataataa taaacctgag tctaataaac ctgaagacaa caataattca
    23881 ggctctactg ataaacctga agacaacaat aattcagact ctactaacaa acctgatgat
    23941 aataacaact caggctctac aagtgaaagt ttttcagctt accaaaaaga agttgttgac
    24001 ttagtaaata tagaaagagc taaagctgga ttaaatcctc ttactcttga ttcttctata
    24061 tcaaatgttg ctacaaaaaa atctcaagat atgattgata acaactattt ttcacacaat
    24121 tctccaactt atggttctcc atttgacatg ttgaaaaaat ttggaataag ctataagaca
    24181 gcaggtgaaa atatagctat gggtcaaaaa actcctaaag aagttgtaaa tgcatggatg
    24241 aattctgaag gtcacagaaa aaatataatg aatccaaatt tcagtaaaat aggtgttgga
    24301 gtagcacaaa aaagtggtgg ttcaatttat tggacacaaa tctttgtagg ataaaataat
    24361 ctttttccat acatatcaaa agagtatctc taagtgataa aaaacacaaa tgagatactc
    24421 ttttgtatct attttaaaat tatcaaatcc aaatatacat actataaata ttatttgttc
    24481 tcttaatttt agtgtgtaga gcaatatata aaaagtattg gcaagatgaa aatggaaaac
    24541 ttaaaaagat atttaatagg taaaaacagg atcttaatat cttcataaaa aaaggctgtc
    24601 tcaaaataga gataaggcct ttttcattgt tgaaatgatt aaaatatatt tgatttcata
    24661 caaaaagagt gcctcatgaa ctaaaaagct cactttgaga cactctcttt tatcaattct
    24721 atataagcat tataatactt taatattatt cagctaattt tttgtatcct tcatatcttt
    24781 ctttagcatc agcttcagct ttagcaaata atccatcagc ttcctctggg aattgtttag
    24841 ctatagcaga atatcttact tgtgcaagta tgaaatcttt gaagcttgca gttggttctt
    24901 ttgaatctaa agtgaatggg tttttacctt cagcttttac ttctggattg aatctgtata
    24961 aatgccagta tccgcaagca acagcttgtg cttcgttagc tacagttctt cccatacctt
    25021 cttttaatcc atggtttata catggagcat aagctattat taaagatggc ccatcatgtt
    25081 tttcagcttc tataacggct ttgataaatt ggtttttatc agcacccata gcaacttgag
    25141 ctacatatac gtttccatat gtcatagcca tcattcctaa atctttcttt ctagattttt
    25201 tacctgaagc agcaaactta gccatagcag cagctggagt agctttagaa gcttgacctc
    25261 ctgtatttga atatacttct gtatcaaata caagtacgtt tacatcttca ccagatgcaa
    25321 gaacatggtc taatccaccg taaccgatat catatgccca accgtctcca cctagtatcc
    25381 attgagatct cttaactaga taatcttttc tatcttttat agcttttact aactctttag
    25441 ctttttcatc tgtacaatca caagctggtt tttctaataa ttcaacaacc ttagcacttg
    25501 ctactttaga agcttctcca tcatttctag aatctaacca ctctgtaaat accgctttag
    25561 catcttcaca gatatccata gaaactaact ctgtcatagc atctactatt ttatttctta
    25621 tttgcttaac agctgtgtac ataccaaatc catattcagc attatcctcg aataaagagt
    25681 ttgcccaaga tggaccttta ccttcatggt tacaagtata aggaattgat ggtgcagaag
    25741 ctccccatat agaagaacat ccagtagcat tagctatcat cattctatct ccatataatt
    25801 gtgtaactac tttaatgtat gcagtttctc cacatccagc acatgctcct gaaaactcca
    25861 ttaatggttg tctaaattga cttcctttaa cagtgtttgc aggcataaca tcaccttttg
    25921 gagctacctc atcaggattt actgcaaatg cccaattatc taactctgta tcaagttgag
    25981 tatctattgg cttcataact aaagcttttt ctttagctgg acatatgtca gcacagtttc
    26041 cacatcctgt acagtcaagt ggactaagtt gcattctata ttttaatcct tctaatcctt
    26101 tacctaatgc ttttttagct tcaaatcctt ctggagcatt agctaattct tcttcagtaa
    26161 ctaatactgg tcttatacat gaatgaggac atataaaaga acattggtta cattgaatac
    26221 agttatctat tatccattca ggaacatcaa cagctatacc acgtttttca taagctgcag
    26281 taccacaagg gaatgttcca tcttctatac cattgaatgt acttactggt aagttgtttc
    26341 cttcttgtgc agtcattggt cttagtatat tttttataaa ttctggttct ttaacttctc
    26401 ctttatctgc atcaacagca ttttcccaag aagctggaac ttcaacttta actaaagcat
    26461 ttattcctgc atcaacagca gcatagttca tgttaactat tttttcacct tttttaccat
    26521 aagctttaac tatagaatct tttaagtatt taacagcatc ttcttctggt attatttcag
    26581 ctaatttaaa gaatgcagat tgcattatca tgtttattct gtttccaaga cctatttctt
    26641 gtcctagttt aacagcgtta actgtataga attttatatc atttttagct atatattgtt
    26701 tcatatgagc tggtaaatgt tcttcaagtc ctgcttggtc ccatatagtg ttaagtacaa
    26761 atgtaccacc ttttttaaga ccttttaata aatcatattg ataaacatat gattgtttat
    26821 gacaggcaat ataatcagct tcatctatta aataagtagc tcttattgga gtatcaccaa
    26881 atcttaagtg agacatagtt ataccaccag attttttaga gtcataatca aagtaagctt
    26941 gagcatattt ttcagtatgg tcaccgatta ttttgatagc ttgtttatta gctccaacag
    27001 taccgtcaga acctaatccc cagaacttac atcttatagt tcctttagaa gctattttta
    27061 ttggctcaga tggagttaaa gaagtattag ttacatcatc aactatacct actgtaaatc
    27121 catttttagg ttgttctgaa actaagttat caaaaacagt ttttatatct gaaggagtag
    27181 tatcttttga acctaatccg tatcttccac ctattatcat aggagcattt tcttttccat
    27241 agaatacatc acgtacatct aagtataatg gttcaccagt tgaacctggc tctttagttc
    27301 tatcaagtac acatattctt tcaacagtag atggcatagc atctaagaaa tgtttcattg
    27361 agaatggtct atataaatga actttaacta aaccatattt ttctccctta gcatttaagt
    27421 aatctatagt ttcttctatt gtttctgtta ctgaacccat agctattaat acgtatttag
    27481 catcttctgc tccatagtaa tcaaataaac tatgttttct acctgttaaa ttactcattt
    27541 tttccatgta tttttcaact atacctacta tattttgata gtatttatta gaagcttctc
    27601 tagtttggaa atatatatct gggttttgag cagtaccacg agcaactgga tgatttggag
    27661 ataaagcatt atctctaaat gcttgaactg cttcaaagtt taataaactt gcataatctt
    27721 cattttctag taattcaact ttttggattt catgtgaagt tctgaatcca tcaaagaaat
    27781 gtataaatgg taatctacct tctattgcag ctaaatgtgc aacaggtgct atatcagcta
    27841 cttcttgaac tgaaccagaa gctaataata cacatccagt ttgtctagca gccataacgt
    27901 cttggtgatc cccaaatatt gataaagctt gagatgctaa agcacgagca cttacatgga
    27961 atactcctgg taacaactct cctgcaactt tatacatgtt aggtatcatt aataataaac
    28021 cttgagatgc tgtaaaagta gaagttaaag ctcctccttg taaagaaccg tggaatgttc
    28081 ctgatgcacc tgcttctgac tgcatttcaa caacattaac tgtttgtcca aatatatttt
    28141 ttcttccttg tgatgcccac tcatcaacta cttcagccat agttgaagat ggtgtgattg
    28201 gatatatagc tgctacatct gtaaacgcat aggcaacatg agctgcagct gtatttccat
    28261 caagtgtttt cataaactta gccataatat gtatcctcct tgatagctac aattgtactt
    28321 gtttataaca agtctatttt tttatattta catataattt acacaacaaa actataaaca
    28381 cacatatagt cgcgcaaata ctctatcttc taatataata ctacagatta gataaaattc
    28441 ctttgaaaat tgtaaattaa ttacatattt aagtagattt tttttaaaat taaatatgtt
    28501 aagt
//
LOCUS       DBGJFF010000042        28398 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.042, whole genome shotgun sequence.
ACCESSION   DBGJFF010000042 DBGJFF010000000
VERSION     DBGJFF010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28398
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.042"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(178..693)
                     /locus_tag="ACOIAF_001837"
     CDS             complement(178..693)
                     /locus_tag="ACOIAF_001837"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HGZ2668850.1"
                     /translation="MDFLAKYKVLKKQNSSKSCLVCGTQNELGLKADFYELENGELVS
                     ICNTKDWHQSYPGRVHGGMSAAILDETIGRAVSINDDQIWGVTVSLELKYKKPVPTDA
                     TIKVVGRITKENRKLFEGTGEIILPNGDIAVTATGKYMKMPIGQIAEGDFSNEEWFFE
                     ESKEKVEYIEL"
     gene            complement(1056..2126)
                     /gene="leuB"
                     /locus_tag="ACOIAF_001838"
     CDS             complement(1056..2126)
                     /gene="leuB"
                     /locus_tag="ACOIAF_001838"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HGZ2668851.1"
                     /translation="MNCNIAVIKGDGVGPEIIDEGIKVLNKICCKFNHRFDCEYVLAG
                     GCAIDETGEPLPNKTVETCRKNEAVLLGSVGGPKWDKCKGDKRPESGLLKLRESLGLF
                     ANLRPATMYESIKEASPLRTDIVEKGIDFVVVRELTGGIYFGERGRKIIDGIENAYDV
                     EIYNENEIRRIGKRAFEIARNRNKKLISVDKANVLESSRLWRSIMEDLAKEFEDVELS
                     HMYVDNAAMQVVKDPSQFDVIVTNNIFGDIISDEASMITGSIGMLPSASLREDSFGMY
                     EPIHGSAPDIAGKDIVNPIATILSVSMMLRHSFNLEEEAKCIEDAVQSVLNKGYRTID
                     IYNGVGNVVGTREMGELIVNEI"
     gene            complement(2133..2624)
                     /gene="leuD"
                     /locus_tag="ACOIAF_001839"
     CDS             complement(2133..2624)
                     /gene="leuD"
                     /locus_tag="ACOIAF_001839"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HGZ2668852.1"
                     /translation="MIANGSVFKFGDNIDTDVIIPARYLNIADYKELATHCMEDIDDK
                     FISKVKKGDIIVATKNFGCGSSREHAPIVIKESGVSCVIASTFARIFFRNSINIGLPI
                     LECEEAANNIDEGDNIEVDFSTGVIKNITKGKEYKAEPFPEFMQNIILNEGLINSIKA
                     NRG"
     gene            complement(2639..3916)
                     /gene="leuC"
                     /locus_tag="ACOIAF_001840"
     CDS             complement(2639..3916)
                     /gene="leuC"
                     /locus_tag="ACOIAF_001840"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HGZ2668853.1"
                     /translation="MGMTMTQKILAKHAKLNEVKKGQLIEADLDVVLGNDITSPVAIR
                     EFEKLGIEDVYDKTKVVMVLDHFTPNKDIKSAEQCKFTRSFAKSKGVVNFFDVGDMGI
                     EHVLLPEKGIVTAGDVIIGADSHTCTYGALGAFSTGVGSTDMGAGMATGKCWFKVPGA
                     IKFVLKNKPNKWISGKDIILHIIGEIGVDGALYKSMEFCGDGVEYLSMDDRFTICNMA
                     IEAGAKNGIFPVDDKTMEYINSHKCSTMTKDVNIYEADEDAVYDEVYEIDLAELKETV
                     AFPHLPENTRTVDEIDKDIKIDQVVIGSCTNGRISDLEVVAEIMKGKKVADGVRVMIF
                     PGTQKVYLEAIEKGYITTFIEAGAAVSTPTCGPCLGGHMGILAAGEKSISTTNRNFVG
                     RMGHVDSEVYLASPAVAAASAITGKISKPSEII"
     gene            complement(3873..5615)
                     /gene="leuA"
                     /locus_tag="ACOIAF_001841"
     CDS             complement(3873..5615)
                     /gene="leuA"
                     /locus_tag="ACOIAF_001841"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HGZ2668854.1"
                     /translation="MKCGKYKKYDKMQIVNRKWPDNEIFKAPIWCSVDLRDGNQSLPT
                     PMSVNEKVRMFKMLIDTGFKEIEVGFPSASNTEYTFLRKLIDENMIPDDVTIQVLTQS
                     RSHLIEKTFESIRGCKKAIIHLYNSTSVLQRDVVFNMSKQEIIDIAVEGAKLFNEEVK
                     KYPETEFTFEYSPESFTGTEMDYALEICEAVIDVWKPTPQKKVIINLPSTVEMATPNV
                     YADQIEWFCKNISCRDSIILSLHTHNDRGTCTAASELGLLAGADRLEGTLFGNGERTG
                     NMDIVNVGLNLYTQGIDPELDFSNIDKIIGIYEDCTKLMVHDRHPYAGNLVHCAFSGS
                     HQDAIRKGMIAMKNRDNDYWEVPYLPIDPHDIGREYKEIIRINSQSGKGGAVYIMETD
                     YGFMIPKNMHSDFGNVVKMESDRIGEELSSEAIFNLFKKEYIEVESPYKVKKYKIKSM
                     DELNYENDDSNDTNMIEMTARISYMGNEQRIVGIGNGPVDSFNNALKQCGMKDYKFRY
                     YWEHALEEGSHSRGVAYVGIEHNNEVYFGVSISENINTAAINALMNAINKSYIEEEIK
                     NGDDYDAENISQTC"
     misc_binding    complement(5739..5985)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(6133..6579)
                     /locus_tag="ACOIAF_001842"
     CDS             complement(6133..6579)
                     /locus_tag="ACOIAF_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668855.1"
                     /translation="MSKNKFIYMNYYDNGYFKKGMTIYMVLALIILATIFIYSTTILY
                     ILCIITAVFLFKQGRDFSNKYEGKVYITLDNHSILINNQCIFSIKSKQSQKFNYKIIE
                     KIEVVKNILNIYTNESNYKIRLRALSLEDEKKLLNIIDEKMKKFKA"
     gene            complement(6600..7319)
                     /locus_tag="ACOIAF_001843"
     CDS             complement(6600..7319)
                     /locus_tag="ACOIAF_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HGZ2668856.1"
                     /translation="MKKSIMDVHCHTLISGHAHSTFKENVEEASKKNIKYLGISDHGS
                     NMPGGPHPFYFYNLHLLPRKVQGVKILRGIEGNIMDYNGNLDVQEDMLQHLDYIIASL
                     HRPCIASGTKEENTNAILRVMDKPKVKIIGHPDDSRYPLDYEPIVKKAKDKNILLEIN
                     NSSLSSNSHRTDTWENVSHMLTLCKTYGARVILGTDSHICYSIGEFENAEKVLEAVDF
                     PDELVINYHEDEIIEFFDINF"
     gene            complement(7437..8711)
                     /locus_tag="ACOIAF_001844"
     CDS             complement(7437..8711)
                     /locus_tag="ACOIAF_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HGZ2668857.1"
                     /translation="MFIMENDLTMQELLDQQEQVFSKVKVGELTTGKITAVRNDEVQL
                     GLDYGFDGIIPISELNIEKNQYIEDIYHIGDEITAVITKVSQKDGTITLSKLQLDKRN
                     DFAELQKAYDEHRIITVNVEKNIDKGVFANYNTYTFFIPISQLDTKFITDTSKFVGLN
                     LEVYIKELDVRKNRLVASHRDVLQERINKEREERRAQIKAEKEAERARIKQEREEEKA
                     RIKAAKEDLFNSLEVGQKRDGKVTKIMPYGAFVDIGGIEGLAHINNLAWTRVESVEDV
                     VSEGQEVEVYVLDVNKETKKIALALKDINNDPWDLIAKEVQIDDVVNAKVLRIIEKGA
                     FVQIKEGVDAYLPISELSDTRVAKVTNVVNIGDEVKVKILDFKPKTKRMLVSIKEATR
                     EPEEDITEYLEVEESLGSIGELFKDKFKDLEV"
     gene            complement(8911..10278)
                     /locus_tag="ACOIAF_001845"
     CDS             complement(8911..10278)
                     /locus_tag="ACOIAF_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HGZ2668858.1"
                     /translation="MENSNTNLGSESVGKLLFKLATPAIIAQIVNVLYNIVDRIFIGR
                     MENGEVAMAGVGVAFPIIIIITACSYLIGMGGGPLAAIKMGEQNNDEAEKIMSNSFSV
                     LVILAILLTIGFKIGKEPLLWMFGASESTIGYSMDYLNIYLIGTVFVQISMGMNTFIN
                     TQGFATTGMMTVAIGALINIILDPIFIFGFNMGVKGAALATIIAQGVSAIWVLMFLFG
                     KKSILKIKKKYMIPKASIILPVLGLGISPFIMQSTESLVLIALNSKLQMYGGDLAVGS
                     MAIMSSIMQILMLPNMGVTQGAQPIISYNYGSGQLDRVKKTFKLCLLSCFTYSTILWL
                     LLMIFPAFFVSIFNKNPQLLSMTSWSIKIYFAGAFMFGIQIACQQTFLALGKATISLV
                     LALLRKIVLLIPLIFILPTFFNEKLFAVILAEPVADITAATITAISFFIFYKCFLSKP
                     KAIKE"
     gene            complement(10528..10965)
                     /locus_tag="ACOIAF_001846"
     CDS             complement(10528..10965)
                     /locus_tag="ACOIAF_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COG2426 family protein"
                     /protein_id="HGZ2668859.1"
                     /translation="MGYLHILILSMVPVTELRGAIPIGIAMGLNPIWVYVFSVIGSTL
                     VSIPLILTFRHVLQFLRGKKLFKGIADVIDKKINSRMKKLKSVSIIGIILFVGIPLPT
                     TGTWTASAIASILKMRIKDAFMGVFLGNLLSGVIVSALSLHII"
     gene            complement(10997..11626)
                     /locus_tag="ACOIAF_001847"
     CDS             complement(10997..11626)
                     /locus_tag="ACOIAF_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HGZ2668860.1"
                     /translation="MKKSVLLLDVDYTVINTDSMIDFFIYSLKNKTFKTIIKLPYIIF
                     ILFMYLIRIIPLKKAKEAIFYPIVDFSEEDLKKFFDDCIMKKINESMKKVIYKNKEED
                     NVIIMITASPYAYMKYFKYYGLADEVIGTEFFYENSRYKNKFIGENCKGIEKVKRIKA
                     VLGKLGIEIDYENSYAYSDSKSDLPMLSLTKNAFLVSKKDGEVIEQINS"
     gene            complement(11660..12547)
                     /locus_tag="ACOIAF_001848"
     CDS             complement(11660..12547)
                     /locus_tag="ACOIAF_001848"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HGZ2668861.1"
                     /translation="MKQTNTSIRLLTKISDYIKLMRIKQYIKNFFVFSAIIFSNNILN
                     INLFLNTFIAFVCFCLMSSSVYALNDAIDMDKDKKHPKKCNRPVASGRISKKSANILF
                     VVLALISVCLSTVVSFNLSIILSLYLINNILYSLKLKNIILLDVFSISLGFILRVYAG
                     CVAISVSLSNWIILCTFFLSLYLALGKRKKEIETLRDDAVEHRKILEDYDIENLNQMM
                     IVILSSTIVCYALYSTSNPEKPHMIFTTIFVVYGVLRYNYIINTTNENNPTDIVLKDN
                     ALKINVILWIITCLIILIF"
     gene            complement(12576..13796)
                     /locus_tag="ACOIAF_001849"
     CDS             complement(12576..13796)
                     /locus_tag="ACOIAF_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6080 domain-containing protein"
                     /protein_id="HGZ2668862.1"
                     /translation="MSKILKYKNEMFLFILVVITYLIITKIFFSKTTIFYDLNNTYDV
                     LLDTDTGVLFNLNVFAISQDNSKHILFSAIISIFAYPIYLFCTSIANPGTTDFNSAYG
                     FGLICLQIITSAMSITLVFNHIKKIKMQRLTLILLTMIMIFSFPQLFMTLNVERFIYS
                     QFSLIFFIVIANKMKGKNSYLVELAAIPLFGITISNIYLYFFNMIFEFKLKIGKMLKH
                     LITFILMAYICVVSTKSYESFMNLGNVIQYDTKFISGEPILEKVAMIIERLLYSVFYF
                     PGASIKKGLFLQNGEVATIPVILTLLALCFCVLSVIENSEKRVPKLCMGIIIFNLTLH
                     GIVGYNLVNSSIMAINFSFAVIILLAYFTKALRKNEKNMYNIFLSLLLVTIVISNVNG
                     FIEILNIGIKSYPV"
     gene            complement(14198..15340)
                     /locus_tag="ACOIAF_001850"
     CDS             complement(14198..15340)
                     /locus_tag="ACOIAF_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HGZ2668863.1"
                     /translation="MRYVEYGKTGKMVSVVGYGGLRFDLEKSNQENADLVKYAYEKGI
                     NYFDTAPGYCDDRSEDIFGLAFRQMIKEGKTDFYVSTKEKPKACDTSEKAIEAVKKSI
                     ERLGVSKINFYHVWNIRKMEHYELAMRPGGQYEGLLKCKEEGLIDHIVFSSHQPGDEV
                     IDILSENKFEGVTMGINILNFPYRLKGAKYAVDNGYGVVAMNPLSGGTIPKYNKELSF
                     LSKDGESTIESALRFNIGISQISISLIGFNKKQDIDDACKIADENRIYSDEDIHDIET
                     RLNKNMNEICTGCGYCKVCPKGINTPAYMLFYNEKQMFKKSDEEMTKLVYGLGHWNYT
                     MNSKAKAKECISCGKCEVECTQHLPIIDRLKEIKKWEEDGANSVKV"
     gene            complement(15481..16347)
                     /locus_tag="ACOIAF_001851"
     CDS             complement(15481..16347)
                     /locus_tag="ACOIAF_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HGZ2668864.1"
                     /translation="MDNYKKVKQVLWIILFANFAVALLKIIIGNQIKSYSMTADGFHS
                     LSDGASNIVGLIGIFFASKPKDKNHPYGHKKFEIITSLFISGMLFVIAIKIILSAVLR
                     IANPVVPAITIESLIALIITLFINIFVCMYEYRIGTKLNSYVLISDSLHTRSDIFVSL
                     GVLVTLVGVKLGFPVIIESIVPIIISAFIIYSAYGIFRPSIGILVDRVAVDEDYIKEI
                     VFEFNEVRDVHNIRSRGSKSSIYIDMHVMVDPFISVEQSHDLTHKIEKQIQEEINENA
                     QVIVHIEPFYSF"
     gene            complement(16681..18228)
                     /locus_tag="ACOIAF_001852"
     CDS             complement(16681..18228)
                     /locus_tag="ACOIAF_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HGZ2668865.1"
                     /translation="MANFFNFILLQKDKIIELLIQHMSLTVTSILIAIIVGVPLGIII
                     SRISSLRKFVLGFVNLVQAVPSMALLGLLVPILGIGSKPAIFMVVVYSLLPIVKNTYI
                     GITSIDPVVLESAKGIGLTRNQTLFKIQFPLALPIIMGGVRISAVTAVGLMTLAAFIG
                     AGGLGYLVFSGVQTVNNNMILAGAIPSCIIALLVDYLFSKIEVAVTPKGLNPKAPKKN
                     YIALKVISVVLVVSMLFVVFSSSFSSKKDTITIGSKDYTEQLILGNVYAELVEKNTNL
                     KVKKNLNLGGSSVAFNAIKSGELDMYVDYTGTLLVNVMRHAPIKDADEAYNVVKDTME
                     KENQLTLLDPLGFNNTYTLAMMPETAEKYGINTISDLTKYGKEFTFSPTLEFENREDG
                     LVGLSRDYGLKFKDVKAMNGSLRYTALDNNESQVIDAFLTDGLIKKFNLKILDDDKNF
                     FVPYYAAPLVREDTLKKYPELEKVMNMLSGKINEETMRELNYQVDELGKSPEEVAHSF
                     LVKEGLV"
     gene            complement(18221..19357)
                     /locus_tag="ACOIAF_001853"
     CDS             complement(18221..19357)
                     /locus_tag="ACOIAF_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2668866.1"
                     /translation="MIEIRNVTKKIGNNVILDDISLVVETGTLVVLIGSSGCGKTTTL
                     KLINKLIKPTSGEIYINGKPISQENEIELRRKIGYVIQNTGLFPHLTIKENIELIPRL
                     KKEKSVEEIEKRTLQLLEMVGLDSDEFLNKYPSELSGGQQQRIGVARAIATDAEIILM
                     DEPFSALDPITRTSLQEQLFSLQDELKKTIIFVTHDMDEALKIADKICIMKDGRIAQY
                     DTPENILRKPANDFVKDFIGEDRVWDNPEYIKARDIMIKNPIAVNSTRTVTQGIEIMR
                     TSKVDSLLIIDRAKTLKGIVTVKDMKDIDDKSILLADIMSSEPLHVNEGDNLVEILNV
                     MNRNSVGYIPVISDENKLVGLITRSSLLSVLSEQFLEMEVSVLG"
     gene            complement(19620..20717)
                     /gene="dinB"
                     /locus_tag="ACOIAF_001854"
     CDS             complement(19620..20717)
                     /gene="dinB"
                     /locus_tag="ACOIAF_001854"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HGZ2668867.1"
                     /translation="MKHYRKIIHIDMDAFYASIEQRDNKKLKGRPVIVGGNPQSRGVV
                     ATCSYEARKFGIHSAMPSAVAYNRCPYAVFVRPRFDVYKSVSEQIRDIFYRYTDLVEP
                     LSLDEAYLDVTKNKKNIDSSIEIAKQIKKDIFREVGLTSSAGVSYNKFLAKIASDLRK
                     PNGLTVITEENAQDFLDKLPVNKFFGVGKVTSNTLKNLGIKTGYDLRCLNLFELENIF
                     KKRGYELYKFARGIDDRPVKPNRVRKSVGAETTLSHNLDIDEEETRNILDELCEEVCH
                     RLKNSEKFGKTLTLKIKYEDFTKITRSLSLEHYIDEYNDIRSGVDNLLRNVEVNGKQI
                     RLLGVTISNLSDKKETYKDITLFEYMDSIQM"
     gene            complement(21107..22612)
                     /locus_tag="ACOIAF_001855"
     CDS             complement(21107..22612)
                     /locus_tag="ACOIAF_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HGZ2668868.1"
                     /translation="MKVGFDHKKYLEEQSKYILERVNNFDKLYLEFGGKLMGDLHAKR
                     VLPGFDENAKIKVLQHMKEKVEVVICVYAGDIERNKIRGDFGITYDMEVLRLIDDLRG
                     YELDVNSVVITRFEGQPATTVFINKLERRGIKVYKHYPTKGYPSDVETIVSDEGYGAN
                     EYIETTKPIVVVTAPGPNSGKLGTCLSQLYHENKRGNVVGYSKFETFPVWNVPVKHPL
                     NIAYEAATVDLKDVNMIDSFHLETYGEMSVNYNRDLELFPVLKKIIEKITGKESIFQS
                     PTDMGVNRVGFGIIDDEVIRKASIEEIIRRYFKTACEYKKGQVDKGAYDRMKMIMEEL
                     NLKPEDRNVVLPARNYSNKLKESADKNDTCPVVALELEDGTILTGKGSDLMNGTAAVV
                     LNAIKHLANISDDMHLISPVILEPIINLKTKTLASRNVSLSCQEVLTALSICAVTNPT
                     AQFAMEKLALLKGCQAHSTTILNRDDEQTFRKLGIDVTCDPEYPSQNLYYS"
     gene            complement(22874..23224)
                     /locus_tag="ACOIAF_001856"
     CDS             complement(22874..23224)
                     /locus_tag="ACOIAF_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6483 family protein"
                     /protein_id="HGZ2668869.1"
                     /translation="MAIVDKIFNEIKLMVARLLLGKKYSEYEEKDLVYNTEEEVILIT
                     LKRLVFQGNVNEAEEILFDKAKSVNSENMQYIAIEFYTMLMEKTDEELEAMNFSKQEV
                     YQGIEDIRKVLNLE"
     gene            complement(23447..23638)
                     /locus_tag="ACOIAF_001857"
     CDS             complement(23447..23638)
                     /locus_tag="ACOIAF_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668870.1"
                     /translation="MLDRFFGEGGFGGCAWWIIILFFLFLAFQDCWAEMDLCAWIPFL
                     ILLLILCECGDVFDGGCGC"
     gene            complement(23756..24670)
                     /locus_tag="ACOIAF_001858"
     CDS             complement(23756..24670)
                     /locus_tag="ACOIAF_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HGZ2668871.1"
                     /translation="MRKIVLKDILFLVTTLSIIVILLLVTNITTNSKIKSTNESYLKS
                     DKNDVSQNNIQSSTKNTAFKTTILSTGKSDCILVEIGNKVIMIDTGEDKNGKQIVDRL
                     KEKGINTLDYLILTHLDKDHIGGVDSVLSSVKVKNIIQANYKKDSKQYDEYIDSLKKA
                     DIEPVLLKDNMNIVINSAEINIHPASKSKYESSNDYSIITNISYGAHKFLFAGDAEEE
                     RLSEFINGNTLKYDFVKMPHHGRYDKLTETFLESISTQYAVITCSEKKEPEEGVLKIL
                     ERLNIKTFLTSNGEVVINSDGKTLSVNQ"
     gene            complement(24815..26311)
                     /locus_tag="ACOIAF_001859"
     CDS             complement(24815..26311)
                     /locus_tag="ACOIAF_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HGZ2668872.1"
                     /translation="MFEFETQLKRLKHEVLTAVARLSIEENLTKENIEKIPYEIIKGE
                     KARYRCCVYKEREVVLERAQLALSLTPNSKYGDINPDSVSLDTDEQIIYIVEAACDRC
                     PINEFTVTELCRGCLAHRCKEACKFGAISYINGRAYINHDLCKACGMCKSSCQYDAIS
                     EVVRPCKSVCPTGALDFNRNTMKAMIHEDNCINCGACMSACPFGAISDKSLIAPVAKK
                     LAKKENMYAIVAPAITGQIDSNITYGQLKNAIKSLGFVDMIEASCGADAVTVHESNEL
                     IERIKSGDNYMTNSCCPGFFKYIEKMFPDEKDRISSTVSPMVATGRLVKKIDSDAKVV
                     FIGPCTAKKSEATEKSLKDSVDYVLTFEELMALFEAFNIDLSKCSNEDINDGSIFGRG
                     FGASGGLAKSVQNYIAEKGLSFDFEPIKVSGPRQIKKTMTMAKLGRLEGNFIEGMMCE
                     EGCINGAGKFLSGVKAKNTFDRKNGQSTKKSVLSNDKIKEFEDVNLER"
     gene            complement(26613..28103)
                     /locus_tag="ACOIAF_001860"
     CDS             complement(26613..28103)
                     /locus_tag="ACOIAF_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HGZ2668873.1"
                     /translation="MFQFETQIHKLRHDVLTAIARLGKEDNLTREAMDNIQYEIIKGE
                     KPTYRCCVYKERAIISERVNVSMGLQPGQDKIDLDTMDIEEDEQIIYILEAACDSCPI
                     NEFTVTDICRGCLAHRCKEACKFGAITHVGGMAYINHELCKACGMCKKACQYDAISEV
                     VRPCKSVCPTNALGFDRENMKAMIHEDKCLNCGACMSACPFGAISDKSLIAPVARKLV
                     QKEKMYAVVAPAITGQVEANVTYGQIKNAIKSLGFVDVLEAACGADAVTVHESLEFAE
                     RMEKGEAFMTNSCCPGFVGYIEKTYPNHVGKISSTVSPMIASGRFIKSHEPDAKVVFI
                     GPCTAKKSEAAKKELKGAVDYVLTFEEIMALFEAFDIDLAKCSNEDIDDASIFGRGFG
                     AAGGLTKAIENYLAEKGIGVNFEPVKISGSREIKKTMTMANLGKLEGNFIEGMMCEGG
                     CINGSGKFLSGAKVKATFDRKNSQSTKKSVLANTKIKDFSNINLEK"
BASE COUNT         9266 a         5155 c         2900 g        11077 t
ORIGIN      
        1 aaaaaatata ctttttatct atataaatta atttttttag tatgtaaatt tgacgctatt
       61 acgataaatc tttttagtat aactaattta tttttatctt gtatttttat tgtatttttg
      121 aattttattt tttctaaata taaaatttat gttttaaaat gtaaaatttt tatttaatca
      181 aagttcaata tattccactt tttctttcga ttcttcaaaa aaccattctt cattagaaaa
      241 atcaccttct gcaatttgtc caataggcat tttcatatac tttccagttg ctgtcactgc
      301 tatatctcca tttggtagaa ttatttctcc tgtaccttca aataattttc tgttttcttt
      361 agttattctt ccaacaactt ttatagtagc atctgttggt actggtttct tatatttcaa
      421 ctctaaagat actgttactc cccatatttg gtcatcattt atacttacag ctcttccaat
      481 agtctcatcc aatattgcag cagacatacc tccatgaact ctcccaggat aactttgatg
      541 ccaatccttt gtattacaaa ttgatacaag ctctccattt tcaagttcat aaaaatctgc
      601 tttaagtcct agctcatttt gtgttccaca aactaaacaa gatttactac tattttgctt
      661 cttcaaaact ttatactttg ccaaaaaatc catctcctaa ccgacattag tagtcgctta
      721 atattaaata attctaaaat atattatata atattaataa aatccctgca aacttctaat
      781 aataaaaata gactacttca atatgataag tttaatttaa agtattattt tccaaaaaat
      841 aactttatca aaatagaaac tcaaagtaga tataagcctc tttttcgtta ttaaaatagt
      901 aaaaatatat ttgacttaat ataaaaaaga gtgtctcatt aactaataaa gtttattttg
      961 agacacccct cataaaacaa attatactac aatagatttt tactaattct acaagttata
     1021 tatttaaatt atattattca aattaaacta taattctaaa tttcatttac tattaattcg
     1081 cccatttccc ttgttcctac aacatttccc acaccattat agatatcaat agttctataa
     1141 cctttattga gtacactttg tacagcatct tcaatacact tagcttcttc ttctaagtta
     1201 aaactatgtc ttaacatcat gcttacagat agaatagtcg caattggatt tacaatatct
     1261 tttccagcta tatcaggtgc acttccatgt attggttcat acataccaaa actatcttct
     1321 ctaagtgatg ctgacggtaa catacctata gaacccgtaa tcatagatgc ttcatcagat
     1381 attatatctc caaatatatt attagtcact attacatcaa attggctagg gtcttttaca
     1441 acttgcatag cagcattatc aacatacata tgtgacagtt ctacatcttc aaactcttta
     1501 gctaagtctt ccattatact tctccaaagt cttgaactct caagcacatt tgccttatct
     1561 acacttataa gttttttatt tctatttctg gctatttcaa aagctctctt accaattctt
     1621 cttatttcat tttcgttata tatttctaca tcataagcat tttcgatacc atcaatgatt
     1681 tttcttccac gttctccaaa ataaatgccc cctgttaatt cccttacaac aacaaaatca
     1741 atgcctttct caactatatc cgttctaagt ggtgaagctt cttttatact ttcatacatt
     1801 gtagctggtc ttaaatttgc aaataaaccc aatgactctc ttaattttaa aagaccagac
     1861 tctggtcttt tatctccctt acatttatcc cactttggtc ctccaacaga accaagcaat
     1921 acagcttcgt tttttcgaca agtttcaaca gttttattag gtagaggttc tccagtttca
     1981 tcaatcgcgc atcctccagc taatacatat tcacaatcga atctatgatt aaatttacaa
     2041 caaattttat ttagaacctt tataccttca tctataattt ccggtccaac accatcacct
     2101 tttatgactg ctatattaca attcatactc cactaccccc tatttgcttt tattgagtta
     2161 attaagcctt catttaatat aatattttgc ataaactcag gaaatggttc tgctttatat
     2221 tcttttcctt ttgttatatt ttttatcaca cctgtagaaa aatctacttc tatattatcc
     2281 ccttcatcta tgttattggc agcttcttca cattcaagta taggaagccc tatatttata
     2341 gagtttctaa aaaatattct agcaaaagta gaagcaatca cacaggatac tccactttct
     2401 tttattacaa ttggtgcatg ttctcttgat gaaccacaac caaagttttt agttgcaact
     2461 ataatatctc ctttttttac cttagaaata aatttatcat caatatcctc catacaatgc
     2521 gttgctagtt ctttataatc agctatattt aaatatctag ctggtattat tacatctgta
     2581 tctatattgt caccgaattt aaaaacactt ccattggcaa tcataaattt tacctccctt
     2641 aaattatttc tgatggtttt gatatttttc cagttatagc tgatgctgct gctacagctg
     2701 ggcttgcaag atacacctct gaatcaacat gccccattct tcctacgaaa ttcctatttg
     2761 ttgttgaaat ggatttttct cctgctgcaa gtatacccat atgtccccct agacatggac
     2821 cacaagttgg tgtagaaact gctgctcctg cctctataaa agtggtaata tacccttttt
     2881 ctatagcttc aagatatact ttttgagttc caggaaatat cataactcta actccatctg
     2941 ctactttttt gcctttcatt atctcagcta ctacttcaag gtctgatatt cttccatttg
     3001 tacaagagcc tattacaact tggtctatct ttatatcctt atctatctca tctactgttc
     3061 tagtattttc aggaagatga ggaaaggcta ctgtttcttt tagttcagct aaatctattt
     3121 cgtatacttc atcatagact gcatcttcat ctgcttcata tatatttaca tcttttgtca
     3181 tagttgaaca cttatgtgaa tttatatatt ccatagtctt atcgtctact ggaaaaattc
     3241 cattttttgc tcctgcttct attgccatat tacaaatagt aaatctatca tccatagata
     3301 agtattctac tccatctcca caaaattcca ttgacttgta aagtgctcca tcaacaccta
     3361 tctcccctat tatatgaagt attatatctt taccacttat ccatttattt ggtttatttt
     3421 tcaaaacaaa ttttattgct ccaggaacct taaaccaaca ttttcctgta gccatacctg
     3481 ctcccatatc agttgaacct acaccagtag aaaatgctcc taatgctcca tatgtgcaag
     3541 tatgagagtc agcacctata attacatctc cagcagttac tatacctttt tcaggaagaa
     3601 gaacatgctc tattcccata tctcctacat caaaaaaatt tacaactcct tttgattttg
     3661 cgaaacttct tgtaaattta cattgttctg cactctttat atctttatta ggtgtaaagt
     3721 ggtctaaaac catcactact ttagtcttat cataaacatc ttcaattcct aatttttcaa
     3781 attctctaat ggctactggt gatgttatat cattacctaa aactacatct aaatctgctt
     3841 cgattaattg ccctttctta acttcattta atttagcatg tttggctaat attttctgcg
     3901 tcatagtcat ccccattttt tatttcctcc tcaatataac ttttatttat tgcattcata
     3961 agtgcattaa tagctgctgt atttatgttt tccgatatac ttacaccaaa atagacttca
     4021 ttattgtgct ctattcctac ataagctacc cctcttgaat gtgacccttc ctctaatgca
     4081 tgttcccagt aatatctaaa tttataatct ttcatgccac attgctttag tgcattatta
     4141 aatgaatcta caggtccatt tcctatacca acaatccttt gttcatttcc catataactt
     4201 attcttgcag tcatctcaat catgttagta tcattagaat catcattttc atagtttaat
     4261 tcatccattg attttatttt atacttctta actttataag ggctctcaac ttcaatatat
     4321 tctttcttaa acaaattaaa tattgcttca ctactaagtt cttccccaat tctatcagat
     4381 tccattttga ctacatttcc aaaatcagaa tgcatattct taggtatcat aaatccataa
     4441 tctgtttcca ttatataaac agctcctcct tttccagatt ggctatttat ccttataatt
     4501 tctttgtatt ctcttccaat atcatgaggg tctattggta aatatggaac ttcccagtaa
     4561 tcattatctc tatttttcat tgctatcatg ccttttctaa tagcatcttg atgtgaacct
     4621 gaaaaagcac aatgtacaag attacctgca tatggatgtc tatcatgtac cataagtttt
     4681 gtacagtctt catatatacc tattatctta tctatattgc taaaatctag ctctgggtct
     4741 attccttgtg tataaagatt aagtcccaca tttactatat ccatattccc agttctttca
     4801 ccatttccaa atagtgttcc ttcaagtcta tcggcaccag ccaataatcc aagttcactt
     4861 gcagccgtac aagttcctct atcattgtga gtatgcaaac ttagtataat agaatcccta
     4921 caacttatat ttttacaaaa ccattctatt tggtctgcat atacgtttgg tgttgccatt
     4981 tcaactgttg atggaagatt tattattact tttttttgag gagttggttt ccaaacatct
     5041 atcactgcct cacatatttc aagtgcataa tccatctctg ttcctgtaaa actctctggt
     5101 gaatattcaa aagtaaattc tgtttctgga tattttttta cttcttcatt aaataatttt
     5161 gctccttcaa ctgctatatc tattatttct tgtttactca tattaaaaac cacatctctt
     5221 tgaagaacag atgtagagtt atataaatgt ataattgctt ttttacatcc ccttattgat
     5281 tcaaatgttt tttctataag atgagaccta gattgtgtaa gtacttgtat agttacatca
     5341 tctggaatca tattctcatc tattaatttt cttaaaaatg tatactctgt atttgatgct
     5401 gatggaaatc ctacctctat ttctttaaat cctgtatcaa taagcatttt gaacattctg
     5461 actttttcat ttacactcat aggtgttggc agtgactgat ttccgtctct taaatcgaca
     5521 ctacaccaaa ttggagcctt aaatatttca ttatctggcc atttacgatt tactatttgc
     5581 attttatcat attttttata ctttccacac ttcatgttat ttcccccttt ttctgttttt
     5641 tgtattcagt atacctcata tcgacgcaat cctgcacaat tcataaggat aaatgtcaat
     5701 tttatcatta gaataaaaaa aacctcatct ctaataaaat tagagacgag gttttgctcg
     5761 cggtaccact ctatttagct ttctaaaaag ctcccttagt aatagccaat acaataatta
     5821 taatgactat atttcctata acgtggaatt actccgatta ttctaagatt taaatcttag
     5881 aaaacagctc ctagacaagt tcagaactat caactactaa atcacaccat cctttagctc
     5941 tctgaaagtt tcattgttct tactacttct attcatcact tttttaatca tttaaatttt
     6001 ctaaatattt tgatttaata gttattatat acacattaaa atgtattgtc aacacaaaaa
     6061 ataattatct caatttttta tttttgataa gcaattctat aaagcatgct aactattgaa
     6121 aacagtatga tattatgcct taaacttttt catcttttca tctatgatat tcaataattt
     6181 tttttcatct tccaaactca atgcccttaa tctaatttta taattagatt cgttagtgta
     6241 tatatttaat atatttttta ctacttctat tttttctatt atcttataat taaacttttg
     6301 actttgttta gattttatac tgaatataca ttgattattt attaatattg aatgattatc
     6361 gagagtaata tatacttttc cctcatattt atttgaaaaa tcacgacctt gtttaaatag
     6421 aaaaactgct gttattatac ataaaatata taatattgtc gttgaatata taaaaattgt
     6481 agctaaaatt ataagtgcta aaaccatata tatagtcatt ccttttttaa aataaccatt
     6541 gtcataataa ttcatataga taaacttatt tttactcaaa ttttttcact cccaagattc
     6601 taaaaattta tatcaaaaaa ttctataatt tcatcttcat gataatttat gactaattca
     6661 tctggaaaat ctactgcttc taaaactttt tctgcatttt caaattctcc tatagaataa
     6721 catatatgag agtcagtacc aagaattact cttgcaccat atgttttaca caatgtcaac
     6781 atatgactta cattttccca agtatccgtt ctatgagagt ttgaacttaa tgatgaatta
     6841 tttatttcta aaaggatatt tttatcttta gcttttttta ctataggttc ataatctaaa
     6901 ggatacctac tgtcatctgg atgtccaatt atttttacct taggtttatc cataactctt
     6961 aaaatagcat ttgtattttc ttcttttgta ccagaagcta tacaaggtct atgcaaactt
     7021 gcgattatat aatccaaatg ttgcaacata tcttcttgaa catctaaatt accattataa
     7081 tccattatat ttccttctat tcccctaaga atctttacgc cttgcacttt acgtggaagc
     7141 aaatgcaaat tataaaaata gaatggatgt ggtcctcctg gcatattaga tccgtggtct
     7201 gatattccta agtattttat attcttctta ctcgcctctt cgacattttc cttgaaagta
     7261 ctatgtgcat gtccacttat tagagtgtga caatgtacat ccataataga ctttttcatt
     7321 aaatttcctc ctaatatttt acataaattc gtgaaaatag ctaaagggct atttcaaaac
     7381 taaagctgtc ataatagaca gtgtaacgtt ttaaaacagc cccattttca attattttac
     7441 acttctaaat ctttaaattt atctttaaat aattctccaa tacttcctaa agattcctct
     7501 acttctaggt attctgttat atcctcttct ggctctctag ttgcttcttt tattgaaacc
     7561 aacattctct tagtctttgg tttaaaatca agaatcttaa ctttaacctc atctccaata
     7621 ttaacaacat tagtaacttt agccactcta gtatcactca attcagatat tggtaaatac
     7681 gcatctacac cttctttaat ttgaacaaaa gccccttttt ctattattct aagaacttta
     7741 gcattgacaa catcatcaat ttgtacttct tttgctatta aatcccaagg gtcattattt
     7801 atgtctttta atgccaaggc aattttttta gtctctttat ttacatccaa cacataaact
     7861 tctacttctt gtccctctga aacaacatct tcaacacttt caactcttgt ccaagctaaa
     7921 ttatttatat gtgctaatcc ttcaatccca ccaatatcca caaatgcacc ataaggcatt
     7981 atctttgtca ccttaccatc tcttttttgt ccaacttcaa gagaattaaa taagtcttct
     8041 ttagctgctt tgatacgagc tttttcttct tctctttctt gcttaattct tgctctttca
     8101 gcttcttttt ctgccttaat ttgagctctt ctttcttctc tctctttatt tatacgctct
     8161 tgtaatacat ctctatgaga tgctacaagt ctattttttc taacatccaa ttcttttata
     8221 taaacttcta agttaagacc aacaaattta gatgtatctg taataaattt tgtatcaagt
     8281 tgagaaattg gtataaaaaa tgtatatgta ttgtaattag caaatacacc tttatctata
     8341 ttcttttcaa catttacagt tattattcta tgttcatcat aagccttttg taactcggca
     8401 aaatcatttc ttttgtcaag ttgtaattta gataaagtta ttgtaccatc tttttgagat
     8461 acttttgtta ttacagcagt tatttcatca cctatgtggt atatatcttc tatatattga
     8521 tttttttcta tgttcaattc acttataggt attattccat caaacccata gtctaatcct
     8581 aattgtactt catcatttct cactgcagtt atttttccag ttgtcaattc tccaactttc
     8641 actttgctga atacttgttc ttgttggtca agtaattctt gcattgttaa atcattttcc
     8701 attataaaca tcctttcctc ttattgatta tcatatctgt aaatattata ccattaatat
     8761 tgcaaatact tacatttttc ttactataat ttttaaaata aattcaactt catatattta
     8821 tacattatag aaaatatctt attaaattaa aatacaaatt ctaattttta tctaataaaa
     8881 tattttcata aaaaatttat attgtatatc ttattctttt attgctttag gttttgatag
     8941 aaaacattta taaaatataa agaaagaaat tgctgttata gtagcagctg ttatatctgc
     9001 aactggctct gctaaaatta ctgcaaaaag tttttcatta aagaatgttg ggagtataaa
     9061 tattaaaggt attaaaagta caatctttct aagtagagct aaaaccaaag atattgttgc
     9121 ttttccaagt gctaaaaatg tctgttgaca agctatttga atcccaaaca taaaagcacc
     9181 agcaaaatat atttttatac tccaactagt catactaagt aactgtggat tcttattaaa
     9241 tatactaaca aaaaatgcag gaaatatcat taataaaagc cataaaatag tagaatatgt
     9301 aaaacatgac aacaaacaca acttaaatgt cttttttact ctatcaagtt gacctgaacc
     9361 ataattataa cttattattg gttgtgctcc ttgtgtaacc cccatattag gaagcataag
     9421 aatttgcatt atactgctca ttattgccat tgaacctact gctaaatctc ctccatacat
     9481 ttgtaattta ctatttaagg ctataagtac taaactttct gtagattgca ttataaatgg
     9541 agagattccc aaaccaagca ctggtaagat gatacttgct tttggtatca tatacttctt
     9601 ttttatttta agtatacttt tctttccaaa gagaaacata agtacccaaa tagcagaaac
     9661 gccttgtgca attatagtag ctaatgcagc tccttttaca cccatattaa agccaaatat
     9721 aaaaatcggg tcaagtatta tgtttattaa agcacctata gcaactgtca tcattccagt
     9781 tgtagcaaac ccttgggtat ttataaaagt attcataccc attgaaatct gaacaaacac
     9841 tgtacctatt aaataaatat ttaaatagtc cattgaatat ccaattgtag attcacttgc
     9901 tccaaacatc caaagtaaag gctcttttcc aatcttaaaa cctatagtca aaagtattgc
     9961 taaaatcacc agtacagaaa aactgttact cattattttc tctgcctcat cattgttttg
    10021 ctcacccatt tttatagcag ctaatggacc tcctcccata ccgattaaat aactacatgc
    10081 agttattata attattattg gaaatgccac tccaacacca gccatagcaa cttctccatt
    10141 ttccattcta ccaataaaaa tcctatcgac tatgttgtat aaaacattta caatttgtgc
    10201 gataatagca ggtgtagcaa gtttaaataa taatttaccc acactttcac tacctaagtt
    10261 agtgtttgaa ttttccattt taaaacctcc tcaatccaat aattaaaatg tatcactaaa
    10321 attttattct atataaaaat ttcattttat tagcagacaa atttagagta ctttccctac
    10381 aagtaatact atactatatt tcttttgcca tagtaaacat tttagaacta actgaagtta
    10441 aatactttat ctcatttttt atttattatt aaaaattttt cacatatact cataaaaaaa
    10501 ctatccaagt atattactta gatagtttta tattatatgt aaagaaagag ctgaaactat
    10561 aacccctgat aacaaattgc caagaaatac tcccataaaa gcatctttta ttctcatttt
    10621 taatatggaa gctatagcag atgcagtcca tgttcctgta gttggcagtg gtatacctac
    10681 aaatagtatt ataccaatta tcgaaacact ttttaatttc ttcattcttg agtttatttt
    10741 tttatcaatt acatcagcaa tccctttaaa tagtttcttc cctcttaaaa attgtaatac
    10801 atgtctgaat gtcaatatca agggaatcga taccaatgta gaacctatta cactaaatac
    10861 ataaacccat ataggattta agcccattgc aatacctata ggaatggctc ccctcaattc
    10921 tgttacagga accatagata aaattaatat gtgtaaatat cccaaactat tccctcctat
    10981 tctaagtgac attaagttat gaatttattt gctctataac ctcaccatcc tttttactaa
    11041 ctaaaaatgc atttttagtc aatgaaagca ttggtaaatc actcttagag tctgaatatg
    11101 catatgaatt ttcataatca atctctatcc caagtttacc taatacagcc tttattcttt
    11161 ttactttttc aattccctta cagttttctc caatgaattt atttttatat cttgaatttt
    11221 cataaaaaaa ctccgtgcct ataacctcgt ctgcaagacc ataatactta aaatatttca
    11281 tatatgcata tggagatgca gttatcataa ttataacatt atcttcttct ttatttttgt
    11341 aaataacctt tttcatagat tcgttaattt ttttcattat gcaatcatca aaaaactttt
    11401 ttaaatcttc ttcactaaaa tctactattg gataaaagat tgcctctttt gccttcttca
    11461 atggtattat tctaattaaa tacataaata gaataaaaat aatgtatggt aactttataa
    11521 tagttttaaa ggttttattt tttaaagagt aaataaaaaa atctatcatg ctatcagtat
    11581 ttataacagt ataatctaca tcaagtaata atacactttt tttcataagt atttccccat
    11641 cctatcacta aaaatatcac taaaaaatca atattattaa acatgtaatt atccataaga
    11701 ttacgtttat ttttaaagca ttatctttta ggacaatatc agtaggatta ttttcatttg
    11761 tagtgttaat tatataatta tatctaagta caccataaac tacaaatatt gttgtaaata
    11821 tcatatgtgg cttttctgga ttacttgtag aatacaatgc ataacataca attgtagaac
    11881 ttaatataac tatcatcatt tgatttagat tttctatgtc ataatcttcc aatatttttc
    11941 tatgttcaac tgcatcatct cttaaagttt ctatttcttt tttacgtttt ccaagagcta
    12001 agtacagaga taagaaaaat gtacataaaa ttatccaatt tgataaacta acacttatgg
    12061 ctacacaacc tgcataaacc ctaagtataa aaccaagaga tattgaaaac acatctaata
    12121 gtattatatt ctttagttta agagaatata atatattgtt tatcaaatag agactgagaa
    12181 ttatagaaag attaaaacta acaactgttg ataaacagac tgaaattaaa gctaatacta
    12241 caaatagtat attagcactt tttttgctta ttcttccact tgccactggt ctattacatt
    12301 ttttaggatg ttttttatct ttatccatgt ctatagcatc attcagtgca tatactgatg
    12361 atgacattag gcaaaaacac acaaacgcaa taaacgtatt taaaaataaa tttatattta
    12421 gtatgttgtt tgaaaaaatt atagctgaaa atacaaagaa attctttata tattgcttta
    12481 ttctcattaa ctttatataa tctgaaattt tagtaagaag ccttatactt gtattggtct
    12541 gtttcataaa cttactaccc tttcaatatt attttttaaa caggatatga ttttatccct
    12601 atgtttaaaa tttctataaa tccattgaca ttactgatta ctatagtaac caataacagc
    12661 gatagaaata tattatacat attcttttca ttttttctaa gcgctttcgt aaagtaagcc
    12721 aataaaatta taactgcaaa actaaaattt atagccataa tacttgaatt gactagatta
    12781 tatcctacta taccatgtaa agttagattg aatataatta ttcccataca tagcttagga
    12841 actctttttt ctgaattttc aataactgat aaaacacaaa aacataaagc taataatgta
    12901 agaattactg gtattgtagc aacttctcca ttttgcaaaa ataacccttt tttaatactt
    12961 gctccaggaa aataaaaaac cgaatacaat aatctctcta taatcattgc aaccttttcc
    13021 aaaattggtt ctccacttat aaatttcgta tcatactgta ttacatttcc aaggttcata
    13081 aaactctcat aagacttcgt tgaaactaca caaatatatg ccattaggat aaatgtaatt
    13141 aaatgtttta acatttttcc tatttttaat ttaaactcaa atatcatatt aaaaaaatac
    13201 aaatatatat tactaatagt tattccaaat agaggaatag ctgctaattc tactaaataa
    13261 gaattttttc ctttcatctt atttgcaata actatgaaaa atattaatga aaattggcta
    13321 tatataaatc tttctacatt taaagtcata aataactgtg gaaaagaaaa tatcattatc
    13381 atagtaagta aaattaaagt taatctctgc atttttatct ttttaatatg attaaagact
    13441 aaagttattg acatagcact agttattatt tgtagacaaa ttagtccaaa cccataggca
    13501 ctattaaaat ctgttgtccc aggatttgct attgaagtac aaaataggta tattggatat
    13561 gcaaaaattg atataattgc tgaaaacaat atatgcttac tattgtcttg acttatagca
    13621 aatacattta aattgaataa aactcctgta tctgtatcta acaatacatc ataagtattg
    13681 tttaaatcat aaaatatagt agtcttagaa aaaaatatct tagttattat taagtaagtt
    13741 attacaacta atataaacaa aaacatctcg tttttatatt ttaatatttt actcatcaat
    13801 atatcaccat ccattcatta tataggattt gctgacacct acaatttatt ccttatattt
    13861 aatagccatc ttcataatgt tcttacttgt tttattgtaa ttgaataact ctttatttac
    13921 aaccacaata tttactttat tgatgttata atcagtcaag tattgtttat ataacataga
    13981 ttagtgttgt ataaagtgag aatttgtaag caatattaat gtaaaatatt tgaaaaactt
    14041 tatataaaaa tatacaatgt tacttatgga attaaactat aacaaattaa agcataattt
    14101 aaggagacaa aattataatc tgtctcctta aatattaaaa ctatatttta aaacattact
    14161 aaatactata caaatttcaa atatagtata aaattaatta gactttaaca ctattagctc
    14221 catcttcttc ccatttttta atttctttta atctatctat tataggtaaa tgctgagtac
    14281 attcaacttc acattttcca caagatatac attctttagc ttttgcctta ctattcattg
    14341 tgtaattcca atgtcctaag ccataaacta actttgtcat ttcttcatca ctctttttaa
    14401 acatctgctt ttcattataa aaaagcatat aggctggggt attaatacct tttggacata
    14461 ctttacaata accacaacct gtacatattt cgttcatatt cttatttaat ctagtctcaa
    14521 tatcatgtat atcctcatct gaataaattc tattttcgtc tgcaatttta catgcatcat
    14581 ctatatcttg ttttttatta aaccctatca aagatatact tatttgtgaa attcctatgt
    14641 tgaatcttaa agctgattct atagtacttt cgccatcttt tgatagaaaa gaaagttctt
    14701 tattatattt tggaatagtt ccaccactta atggattcat tgccacaaca ccatatccat
    14761 tatcaacagc atatttggca ccttttagcc tatatggaaa gtttaatata tttattccca
    14821 tagttacacc ttcaaactta ttttcactta gaatatctat aacctcatct cctggttgat
    14881 gagatgaaaa tactatatgg tctatcaatc cttcttcttt acatttcaat aatccttcat
    14941 attgacctcc aggtctcatt gctagttcgt aatgttccat ctttctaata ttccaaacat
    15001 gataaaaatt tattttagat acgcctaatc gttctattga tttttttaca gcctctatag
    15061 ctttttctga tgtatcacat gcttttggct tttctttagt tgatacataa aaatcggtct
    15121 taccttcctt tatcatttgt ctaaaagcta gtccaaatat atcttcactt ctgtcatcac
    15181 aataccctgg tgctgtatca aagtaattaa tacctttttc atacgcatat ttgactaaat
    15241 cagcattttc ttgattactt ttttctaaat caaaccttaa tcctccatag ccaactactg
    15301 aaaccatttt ccctgtcttt ccatattcaa cgtatctcat tgtaaattcc tccttagata
    15361 tattaagact taatttcttt atagaaaata ttataccata aataattttt ataaattatt
    15421 tatggtataa tatttttttt ataaattata tttttttata acttgtattt tataactaag
    15481 ttaaaaacta taaaaaggtt caatatggac tattacttga gcattttcat taatttcttc
    15541 ttgtatttgt ttctcaattt tatgtgttaa atcatgagat tgctctacgc ttataaatgg
    15601 gtctaccatt acatgcatgt caatataaat agaacttttg ctacctctac ttctgatatt
    15661 gtgaacatcc cttacctcat taaattcaaa cacaatttct tttatataat cctcatctac
    15721 agccactcta tcgaccaaaa ttccaataga tggcctaaat attccataag ccgaataaat
    15781 tatgaatgca gaaataataa taggaactat agattctatt attactggaa atcctaactt
    15841 aacaccaact aaagtaacca atacacctag agacacaaat atatcacttc ttgtatgcaa
    15901 tgaatccgaa atcaaaacat aactatttag tttagtccca attctatatt catacatgca
    15961 tacaaaaata tttataaaaa gtgtaataat taaagcaatt aaactttcta tagttatagc
    16021 aggtacaact ggattagcta ttcttaacac tgcactaaga attatcttaa ttgctataac
    16081 aaacaacata ccacttataa ataaactggt tataatttca aactttttat gtccataagg
    16141 atgattttta tcttttggtt tagatgcaaa gaaaatacct ataagcccca ctatattaga
    16201 tgctccatct gacaaagaat gaaatccgtc tgctgtcata ctatagcttt ttatttgatt
    16261 accaataatg atttttaata atgctactgc aaaattagca aataaaatta tccacaatac
    16321 ttgcttcact ttcttatagt tatccataac aaattccccc tcccttcgat tcaataaaga
    16381 aagctatcta tcaatatcaa tcaaccagac aataaaatcg tatataagca catttactat
    16441 atttatattc aaaattggtt gtacttgttc attctatttg aaacttaatt ataaataaaa
    16501 atcgtaggta tggttttggt atacataaaa ataagagact gtctcaaaat aaagataatc
    16561 ttctttttta ttgttgaaat gataaaatat atttgatttc atataaaaaa gagtgcctca
    16621 tgaactaaaa agttcatttt gagacactcc cttaaatttt tgatataaac tattaatttt
    16681 ttatactaag ccttctttaa ctaagaatga atgagcaact tcttctggtg atttaccaag
    16741 ttcatccact tgatagttaa gttctctcat agtttcttca tttatttttc ctgaaagcat
    16801 attcataact ttttctaact caggatattt cttcaaagta tcttctctta ctaatggtgc
    16861 tgcataatat ggtacaaaga aatttttatc atcatctaat atcttaagat taaacttttt
    16921 aataagacca tcagttaaaa atgcatctat aacctgagat tcattattat ctaaagctgt
    16981 atatcttaaa cttccattca ttgcttttac atctttgaat tttaaaccat aatctctaga
    17041 aagtcctaca agaccatctt ctctattttc aaactccaaa gttggtgaga atgtaaattc
    17101 tttaccatat ttagtcaaat cagaaatagt atttattcca tacttttcag cagtttcagg
    17161 catcatggct aatgtgtaag tattattaaa tccaagaggg tcaagtaaag ttaactgatt
    17221 ttctttttcc atagtatctt ttacaacatt atatgcttca tcagcatctt taatcggtgc
    17281 atgtctcatt acatttacca aaagtgtacc tgtatagtca acatacatgt ctaactcacc
    17341 agatttaatt gcattaaatg caacagatga accacctaga tttaaattct tcttaacttt
    17401 aagattcgta tttttttcta ctaattcagc ataaacattg ccaagtataa gttgttctgt
    17461 ataatcttta gaaccaatag ttattgtatc ttttttactt gaaaaactag aactaaaaac
    17521 aacgaacaac attgaaacaa caagcactac agatattact ttaagtgcta tataattctt
    17581 cttaggtgct tttggattta atccttttgg agtaactgca acttcaattt tactaaataa
    17641 ataatcaact aacagtgcaa ttatacatga tgggattgcc cctgctaaaa tcatattatt
    17701 atttacagtt tgaacccctg aaaatactaa atatcctaaa ccaccagcac ctataaatgc
    17761 tgcaagagtc atcaaaccaa cagctgtaac tgcagatatt ctaactccac ccattattat
    17821 aggaagcgct aaaggaaatt gtattttaaa taatgtctga tttcttgtaa gaccaatacc
    17881 tttagctgat tctaatacta ctgggtcaat acttgttata cctatatacg tattttttac
    17941 aattggtagt aatgaataaa ccactaccat aaatatggct ggtttacttc ctataccaag
    18001 tataggtaca agaagaccca aaagtgccat agacggaact gcttggacta agtttacaaa
    18061 tccaagtaca aactttctaa gagatgagat tcttgaaata attattccaa gaggaacacc
    18121 aactattata gctattaaaa tagatgtaac agttagactc atatgttgta ttaaaagctc
    18181 tataatctta tctttttgta atagtataaa gttaaaaaaa ttagccaaga acactcacct
    18241 ccatttctaa gaattgttca cttaaaacag aaagtaaact acttctagta ataagcccta
    18301 ccagtttatt ttcatcagaa ataaccggaa tatatccaac cgaattacga ttcattacat
    18361 ttagtatttc tactaaatta tcaccttcat ttacatgcaa aggctcactt gacataatat
    18421 cagcaagtaa tattgactta tcatcaatat ctttcatatc ttttacagta acaatacctt
    18481 ttaatgtttt tgctctatct ataattagta agctatcaac cttactcgtt ctcataattt
    18541 ctattccttg agttacagtt ctagtagaat taacagcaat aggatttttt atcataatat
    18601 ctcttgcttt tatatattct ggattatccc aaactctatc ttctccgata aaatctttta
    18661 caaagtcatt tgcaggcttt ctaagtatat tttcgggagt atcatattgt gctattcttc
    18721 catccttcat aatacaaatt ttatcagcta tctttaaagc ctcatccata tcatgcgtta
    18781 caaatattat agtttttttc aattcatctt gaagagaaaa taattgttct tgtaaagatg
    18841 ttctcgttat tgggtctaaa gcactaaaag gctcatccat caatattatt tcagcatcag
    18901 tagcaatggc tcttgcaaca ccgattcttt gttgctgtcc accactaagt tctgaaggat
    18961 acttattcaa aaattcatca ctatcaagtc ctaccatctc aagtagttgc aacgttcttt
    19021 tttcaatttc ttcaacggat ttttcctttt ttaggcgtgg aattagctct atattttctt
    19081 ttattgtaag atgagggaat aggcctgtgt tctgtataac atatcctatt tttcttctaa
    19141 gctctatttc attttcctga gaaattggtt tgccattaat atatatctca ccagatgtag
    19201 gttttattaa tttgtttata agcttcaaag tagtagtctt tccacaacca cttgaaccaa
    19261 tcaaaactac caatgtacct gtttctacaa ctagagaaat atcatctaat ataacattat
    19321 ttcctatttt ttttgttaca tttcttatct ctatcataat ttatcatccc ctttttcgaa
    19381 caagcaacct tcttcaatat tataagttgt ttttataatt atatcatact attttattta
    19441 tgtaaacaaa gtacgatttt taggtcacta tttgtctgaa tattgctaca aatataaagt
    19501 atttatatat agtatataat atataatgtc aaaaaacaaa aaatataaaa atattcataa
    19561 aataacacta gcctcaatta tatagtatta aagctagtgt tatttcattt aaacaaaact
    19621 cacatttgga tactatccat gtattcaaat aatgtaatat ctttataagt ttctttttta
    19681 tcacttaggt ttgatattgt aactcccaat agcctaattt gttttccatt aacctcaaca
    19741 tttctcaaca aattatctac accacttctt atatcattat attcgtcaat atagtgttct
    19801 aaactaaggc ttctagttat ctttgtaaag tcctcatatt taatttttaa tgttaatgtt
    19861 tttccaaact tctcagagtt ttttaatcta tgacatactt cttcacaaag ttcatcaaga
    19921 atgttgcgtg tttcttcttc atcaatatcc aaattatgac ttaatgtagt ctcagcccca
    19981 acagattttc taaccctatt tggtttcact ggtctatcgt ctattcctct agcaaattta
    20041 tataattcat aacctctttt tttgaatata ttttcaagtt caaataaatt taaacacctt
    20101 aaatcatatc cagtttttat acctaaattt ttaagagtat ttgaagtaac ttttccaaca
    20161 ccaaaaaatt tattcacagg taatttatct aaaaagtcct gggcattttc ttcggttatt
    20221 acagttaacc catttggctt tcttaagtct gatgcaatct tagcaagaaa cttattgtat
    20281 gacacaccag ctgaagaagt aagaccaact tctctaaata tatctttttt tatttgcttt
    20341 gctatctcaa ttgatgaatc aatatttttt ttattttttg ttacatctaa gtaagcttca
    20401 tctaaagata atggttcaac taaatccgtg tatctataaa atatatctct tatttgctct
    20461 gacacactct tataaacatc aaatcgaggt cttacaaaaa ctgcatatgg acacctattg
    20521 taagctacag ctgatggcat agctgaatgg ataccaaatt ttctagcttc ataagaacac
    20581 gttgcaacca cacctcggct ttgtggattt ccaccgacaa taacaggtct tccttttaat
    20641 tttttattat ctctttgttc tatagatgca taaaaagcgt ccatatctat atgaattatt
    20701 tttctataat gtttcattat tatcaccttt taaagtataa atagattaat tttatttata
    20761 ttacttttta attataccaa acttatgttt ctttttggta ttttttcatt attttcttta
    20821 tatattcata ttttccataa ataaaaccat ctataatcca ttaacttcaa tataatttat
    20881 aaaaaatcct taaaaacact tgcaaaaact atataaaagc tttacaatag gtattccaag
    20941 gaactaaatt taaaattcct tatggataac agtatatata tttaatttat gggatgtttt
    21001 aaaaacaaaa gactgtctta tgcaagacag cctactgatt ttattataat tgtaatggac
    21061 tgctcaaact attactttaa aacagcccat atttttttat ataattttaa ctatagtaaa
    21121 gattttgtga aggatattct gggtcacaag ttacatctat accaagcttt ctaaatgttt
    21181 gttcatcatc tctatttaat atagtagtag aatgagcttg acaccctttt aataatgcta
    21241 acttttccat agcaaattgt gcagttggat tagttacagc acaaatacta agtgcagtta
    21301 acacctcttg gcaactaagt gaaacatttc tactagctaa agtctttgtt tttaagttta
    21361 tgataggttc taatattaca ggtgatatta agtgcatatc atcagatata ttggctaaat
    21421 gtttaatggc atttaatact actgctgcag ttccattcat taaatcagaa cccttaccag
    21481 ttaatattgt accatcttct aattctaatg caactacagg acaagtatca ttcttgtcgg
    21541 cactttcttt caatttatta ctgtagtttc tagcaggtaa tacaacattt ctatcttctg
    21601 gctttaagtt aagctcttcc ataatcattt tcattctatc ataagcacct ttatcaactt
    21661 gtcccttttt atactcacag gcagttttaa agtatcttct tattatttct tctatagaag
    21721 cttttcttat tacttcatca tctattattc caaagccaac tctattaaca cccatatcag
    21781 ttggagattg gaaaattgat tcttttccag ttatcttttc tattattttt ttaagcactg
    21841 gaaataattc caaatctcta ttgtagttta ctgacatttc accatacgtt tcaaggtgga
    21901 atgaatctat catattcaca tcttttaaat caactgtagc tgcttcataa gctatattca
    21961 aaggatgttt tacaggtaca ttccaaactg ggaatgtctc aaacttagaa tatccaacta
    22021 cattacctct tttattttca tgatacaact gacttagaca agtaccaagt tttccactat
    22081 taggtcctgg tgctgtgact actactattg gctttgtagt ttctatatat tcatttgctc
    22141 catatccctc atcactaact atagtctcaa catctgacgg atatcccttt gttggataat
    22201 gcttataaac tttaattcct ctacgttcta atttatttat aaatacagtt gtagcaggtt
    22261 gtccttcaaa tctagtaatt actacactat ttacatctag ttcataacct cttaaatcat
    22321 ctattaatct taaaacttcc atatcataag tgataccaaa gtctcctctt attttgtttc
    22381 tttctatatc tcctgcatag acacaaatta caacttcaac tttttctttc atatgttgta
    22441 atacttttat ctttgcattt tcatcaaatc ctggaagtac tctttttgca tgaaggtctc
    22501 ccatcaattt tcctccaaac tctaaataaa gtttgtcaaa attatttact ctttctaaga
    22561 tatatttaga ctgctcttct aaatattttt tgtggtcaaa tcctactttc atcttaacac
    22621 ctcactaaat taaatttctt attgataaca gtatatttat atatattttt ttagtaaaac
    22681 atcttataaa aaaatgttaa tactttttca ctcaaagtaa tattatacaa ttaagattaa
    22741 tttttttcaa gatagtttta tggtaaataa gatataccta atataactaa taaaataaga
    22801 atactatatt tttatatata agtataatat tctcacctta gcattaattt atttcaatat
    22861 tttcatatat cttttattct aaattcaata cttttctaat atcttctata ccttgataaa
    22921 cttcttgttt agaaaaattc atagcttcta attcttcatc agtcttttcc ataagcatag
    22981 tataaaattc tattgctata tactgcatat tttcactatt tacactctta gctttatcaa
    23041 ataaaatttc ttctgcttcg tttacatttc cctgaaaaac taatcttttt aaagttataa
    23101 gtataacttc ttcttctgta ttatacacta aatctttttc ttcatattca ctgtattttt
    23161 ttcccaataa aagtcttgca accataagtt ttatctcatt gaaaatctta tccactatag
    23221 ccatttttac atctcctata aataaatttt attttctttt taattatatc agactttatc
    23281 gtaaaaatta ttatatgtaa atgaaataat tcaaaaataa attttaacaa taaaaaaaag
    23341 aaggtagcgg gggatttccg actaccttca aacactagat tgtgattggt ttggtatttt
    23401 atttataccc accataagct ttattaaatc atctaaattg atttttttag catccacatc
    23461 ctccatcaaa aacgtcacca cattcacaaa gtattaataa taatattaag aaaggtatcc
    23521 atgcacataa gtccatttca gcccaacaat cttggaatgc taagaataag aagaataata
    23581 ttattatcca ccaagcacat ccaccaaatc cgccttcacc aaaaaatcta tccaacataa
    23641 tatgttacct cctagtcatt ttgtatgtgg agtttcagct cctaaaatat actatgttta
    23701 aggggtaata tttgctaatg atttttttaa atttttttag atatttttaa aaaaattatt
    23761 gattcacact caatgtctta ccatcactat tgataactac ctctccatta gaagtcaaaa
    23821 atgtttttat attcaatctt tccaatattt ttaaaacacc ttcctctggt tcttttttct
    23881 ctgaacatgt aataacagca tattgggttg aaatagactc taaaaatgtt tcagttaatt
    23941 tgtcatatct tccatgatga ggcattttaa caaaatcata ttttaaagta tttccattta
    24001 taaactctga caatctttct tcctctgcat ctcctgcaaa taaaaactta tgtgctccat
    24061 agcttatatt agtgattatt gaataatcat ttgaactctc gtacttgctt tttgaagccg
    24121 gatgtatgtt tatttctgca ctattgatta caatattcat attatctttt agtagcactg
    24181 gttctatatc agctttttta agtgagtcaa tatattcatc atattgctta ctatctttct
    24241 tataatttgc ttgtataata ttctttactt taacactaga taacacacta tctactccac
    24301 ctatatggtc cttatctaaa tgagttaaaa ttaaatagtc aagtgtattt attccttttt
    24361 cttttaacct atccactatt tgttttccat ttttatcttc tcctgtatca atcattatga
    24421 ctttgttacc tatctcaacc aatatacagt cagactttcc agtacttaat atagtagtct
    24481 taaatgcagt attttttgtg ctactctgaa tattattttg actcacatca tttttgtcac
    24541 tttttaaata agattcattc gttgatttta tcttactatt agtagttata tttgttacaa
    24601 gcaataatat tactattata gataatgtag taactaaaaa caggatatct tttaatacta
    24661 ttttcctcat ccaaaagctc ctataaattt tatttattac atatttatag tatagcaacc
    24721 ttaactctgt aaaatacaca tataaaaagg gcaaatttta ggcatatttg tggcatgata
    24781 aaaacatgat aaaaaaatga ctgtaccaaa acaattatct ttctaaattt acatcttcaa
    24841 attctttaat cttatcatta gataaaacac tctttttagt agattgtcca ttttttctat
    24901 caaaagtatt tttagctttt acacctgata aaaacttacc tgctccatta atacaacctt
    24961 cttcacacat cattccttct ataaaattac cttctaatct tcccaattta gccattgtca
    25021 ttgtcttttt aatttgtctt ggaccagata cttttatagg ttcaaaatca aaactaagcc
    25081 ccttttcagc aatatagttc tgtactgatt tggcaagtcc accagatgct ccaaatcccc
    25141 ttccaaaaat cgaaccatca tttatatctt catttgaaca ttttgataaa tctatattaa
    25201 atgcttcaaa taatgccatt agttcttcaa aagtaagtac ataatctact gaatctttta
    25261 aacttttctc tgttgcctca ctctttttag ctgtacatgg acctataaat acaactttag
    25321 catcagaatc tatttttttt actaatcttc ctgttgctac cataggagac actgtagatg
    25381 atattctatc tttttcatct gggaacatct tctctatata cttaaaaaat cccggacaac
    25441 atgaatttgt catgtaatta tctcctgatt ttattctttc aataagttca ttgctttcat
    25501 gaactgtaac tgcatctgct ccacaagaag cttctatcat atctacaaat cccagtgatt
    25561 ttatagcatt ttttaactga ccatatgtaa tattactatc tatttgacct gttatagctg
    25621 gagctactat tgcatacata ttttctttct ttgcaagttt ttttgcaaca ggtgctatca
    25681 aactcttatc agatattgct ccaaaaggac atgctgacat acatgctcca caatttatac
    25741 aattatcttc atgtatcatt gctttcatag tgtttctatt aaaatccaaa gctcctgttg
    25801 gacaaacgct tttacaaggt cttacaactt ctgatatagc atcatattga catgaacttt
    25861 tgcacattcc acaagcctta cataaatcat gatttatata tgctctccca tttatataag
    25921 aaattgcccc aaatttacat gcttccttac atctatgagc caaacaccct ctacaaagct
    25981 ctgtaactgt aaattcattt attggacatc tatcacatgc tgcttcaact atatatataa
    26041 tttgctcatc tgtgtccaaa ctaactgaat ctggatttat atctccatat ttactatttg
    26101 gagttaaact taatgcaagt tgcgcccttt caagaactac ttctctctct ttataaacac
    26161 aacatctata tctcgccttt tctcctttaa ttatttcata tggaattttt tctatatttt
    26221 cttttgttaa attttcttca atagatagtc ttgctactgc tgttaaaact tcatgtttta
    26281 acctttttag ttgcgtttca aattcaaaca taagtaatcc tccctataaa tcacaccttt
    26341 ttgctatatt tctactatta tgtatattgt attttttatt tatttctata aattattata
    26401 acataggcat atactttgtt aagttatata caaaatatta tatatgtaat ttttatttta
    26461 agtgtatgta atttttattt taagtgtata taatttttat tttagtcgtt caataattaa
    26521 atatataaat aaaaatagag ttgtccaaaa gttaaacttc aacttagaac aactctatta
    26581 acaatatgta tttttgttgc tttttattat ctttattttt ctaaatttat attactaaaa
    26641 tcttttatct ttgtatttgc taagacactt ttcttagttg attgagaatt ttttctatca
    26701 aaagtggctt taacctttgc acctgataag aatttaccag aaccatttat acaaccacct
    26761 tcacacatca ttccttctat aaaattacct tctagctttc ctaaattagc catagtcata
    26821 gttttcttga tttctcttga accagaaatc tttacaggtt caaaattaac accaatacct
    26881 ttttcagcaa gataattttc tatagctttt gtaagaccac cagcagctcc aaaacctctt
    26941 ccaaaaattg atgcatcatc tatatcttca tttgaacact tagccaagtc tatatcaaat
    27001 gcctcaaata atgccattat ttcttcaaaa gtaagtacat aatcaacagc tccttttaat
    27061 tctttcttag ctgcctcact ctttttggct gtacatggtc ctatgaatac aactttagca
    27121 tctggttcat gactttttat aaatctacca ctagctatca taggagatac tgtagaagat
    27181 attttcccta catgatttgg gtatgttttt tctatataac caacaaatcc tggacaacaa
    27241 gaatttgtca taaaagcttc tcccttttcc attctttcag caaattctaa gctttcatga
    27301 actgtaactg catctgctcc acaagctgct tctaatacat caacaaatcc tagtgatttt
    27361 atagcatttt ttatttgacc atatgtaaca tttgcttcaa cttgacctgt tatagctgga
    27421 gctaccaccg catacatttt ctctttttga actaactttc tagctacagg agctatcaaa
    27481 ctcttatcag atattgctcc aaatggacat gctgacatac atgctccaca gtttaaacat
    27541 ttatcctcat gtatcatagc tttcatattt tctctatcaa atcctaaggc attagttgga
    27601 caaacactct tacatggtct aacaacctct gagatggcat catattgaca agctttttta
    27661 cacattccac aagccttaca taattcatga tttatatagg ccattcctcc gacatgtgtt
    27721 atagctccaa atttacaagc ttctttacat ctatgtgcta gacatcctct acaaatatct
    27781 gttactgtaa attcatttat tggacaactg tcacatgcag cttccaaaat atatataatt
    27841 tgttcatctt cttctatatc cattgtatct aaatctatct tatcttgacc tggttgtaac
    27901 cccatactta catttactct ctcagatatt atagctcttt ctttataaac acagcatctg
    27961 tatgtaggtt tctcaccttt tattatttca tactgaatat tatccattgc ttctctggta
    28021 aggttatctt cttttccaag tctagctatt gcagttaaaa catcatgtct taatttgtgt
    28081 atttgtgttt caaattgaaa cattttaata ccacccccgt aaatgatttt atacgatacc
    28141 attataacac aatatgtcct tttattaata tatacataaa atattatatg tttaaatttt
    28201 attttatcga atatatatag tgtttttttg ttttattata taatattatt gatatttctg
    28261 aaatttcact atttttattt ttacttttca atctaatata taatattttt tattatatat
    28321 tttaatttta ctttttactt acataccttt ttgttacatt ttttgatatt aaatttatta
    28381 tctacattta cattttcg
//
LOCUS       DBGJFF010000043        27733 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.043, whole genome shotgun sequence.
ACCESSION   DBGJFF010000043 DBGJFF010000000
VERSION     DBGJFF010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27733
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.043"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            46..120
                     /locus_tag="ACOIAF_001861"
     tRNA            46..120
                     /locus_tag="ACOIAF_001861"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:78..80,aa:Thr,seq:tgt)
     gene            complement(280..957)
                     /gene="srtB"
                     /locus_tag="ACOIAF_001862"
     CDS             complement(280..957)
                     /gene="srtB"
                     /locus_tag="ACOIAF_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtB"
                     /protein_id="HGZ2668874.1"
                     /translation="MKKLYRIVINIILVLVILYSGFNIYSKLTKYNHDTKISSELQKK
                     EYKKEDLSKINSDFKFWLSVENTNINYPVVQSKDNSYYLDKDFYKKDSISGTLFMDYR
                     NKSIDDKNIIIYGHNMKNKTMFNNLNKFKDADFFKKNNKIKITLNGKEFLYDVFSAYI
                     VESDYDYLKTNFNNESDYQNYINDITSKSLYKSPIKVNSNDKIVTLSTCTYEFDDARM
                     VIHGRLI"
     gene            1089..1423
                     /gene="rnpB"
                     /locus_tag="ACOIAF_001863"
     ncRNA           1089..1423
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACOIAF_001863"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            1701..4244
                     /locus_tag="ACOIAF_001864"
     CDS             1701..4244
                     /locus_tag="ACOIAF_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HGZ2668875.1"
                     /translation="MKVLSENNIIRNTVLNVTASFLKQESKINEKLDGVLEKKFEKVE
                     FNEAKYAELLKFNILFYKTLARNTEPLIGKWIVDKYIPEIDELEKDLELTTAKCRKYV
                     NKAMKEGLDSLKANDLNSFLAYDKMELSERRRRLEKDYKVLNLYKDLLNISLRKISLE
                     KKECGDLFLKNQADAKRELKREIIFCVNKILASNKDVVPNNVEENNEQDKVEIKATEI
                     EDLQLGKDNSENDMLLNEIKNSKVKHESKSNDIESKFIDKLNSLNNESVSQTIHNEYK
                     KLCGLEELHSVEGYGFGKEIIKDFACATVVLEFLKRRNRDLIEGAMRLTIIGEFGPEN
                     FKEFVDYVIKNKTEISEDTWNKAQNLIKDNYSELENHEIASKRTRRNKDIDIEEYIYM
                     IKNADKDICFRSSISIEDDAEEGTKEEVDSNNQDIGDVVEDKDTTDKEYDSNKEDIIE
                     PENKKSKKKAKLFGFIKKDNEEVEQEEENLNDISPDIILDKPVENHQVKSEEIEQNEL
                     KEIKQEEPSQHIEEERSVKIEKPINNNLDEKVSSNNESKLEKESKNLEDKKAKEIKEE
                     KLENEKSVVIPIKKKENSNKKSKNSSKDKYRENKKEMKNYVSDKEDSLDDEEVVSKKS
                     RLKETIIAVVIVVIVGVGYFITVGNNKKNDKENIPKSSTQQQANNKLTEEEKKAQAEK
                     EKKEAEEKAKAEKEAQEKAQAEKKAKEMEAYKDGKGVYYTVYAGSLKVEKTAKETAKE
                     YEAKGISSTIIQENGYYKIKIGDYSQYGEAQEKCNELAKKSIDTYIAMYDKYYDYKLE
                     ELKESAPSLSAEELKQKYEDLRSELKNKSGYREYVKHLDKLYEEIVEGA"
     gene            4367..6358
                     /locus_tag="ACOIAF_001865"
     CDS             4367..6358
                     /locus_tag="ACOIAF_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668876.1"
                     /translation="MLKLGEKIIYELSDGFELSGISRYSKEYKKLHITNLGNIIISKS
                     DEVNEDTGVKYIYSFSDNKEKLEAVLEDSQIIVYGNNLPFYIKSENSSKNVTANLTMK
                     ITLDEYDLICKSQREFIFYLKDNMTILSLDDNKFYMGGINKDNEKFIFISGKNRFEIN
                     FDDIERYILEDKRVSLKGYFHMEREGIIVRSVSIFNNNIDSVVPSDLNERVKDNQKIG
                     NLPKDCEIVFCKISGNIDGFDYKNTNMLLVKYQDQLIFINKKSKKTIVKSAKDNCSKL
                     NLGEDIILYDNKNVFNLHINDKNREIMQIDNLKDIENEIVGYTLKHAPFFIQEDFDSL
                     TILKSFQKEIISIKNSDIKDIVINKELENENSNFVETEIKFNNQKVLLNLSKSMVQKL
                     MQDVFIYAKQPLLKENSIEVIYKNWSKAMNDMIIFNFFGNIYYMKSEFDKILEKELND
                     EIRIEVINSLYKQIQEQRNNLDLLSAYMPRILENQEIDLFEKYNTKLDVQVFKQIKNL
                     LSDLSYNISSYLNEVEKSLDNIIFVISGEDKKKYNYRMLKESESASLDVFLKQAISRL
                     NHLVENMYPYYVDETSREMFKLFELLWKNYRNIDDDSIKEILFERITNTYVFKQLTLN
                     NSTKERRKDIIEKIYNSVDYGTNKLDENMFFTGGIKYVK"
     gene            6348..6668
                     /locus_tag="ACOIAF_001866"
     CDS             6348..6668
                     /locus_tag="ACOIAF_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668877.1"
                     /translation="MSSKYNVWTFNYEFLGLDSGDEKNNEVYYEIDLDEILNENYIDE
                     DIDSGLDSDGNSILGELISEMDIDEDVSVDMTYQELEVDLEDLDSYLDEDIDSDIKGI
                     LDEI"
     gene            6860..7735
                     /gene="galU"
                     /locus_tag="ACOIAF_001867"
     CDS             6860..7735
                     /gene="galU"
                     /locus_tag="ACOIAF_001867"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HGZ2668878.1"
                     /translation="MQMTVKKAIIPAAGLGTRFLPATKSQPKEMLPIVDKPTLQYIIE
                     EAIESGIEEILIITGRNKKSIEDHFDKSVELELELEQKGKTEMLEMVRDISNMVNIHY
                     IRQKEPKGLGHAIYCAKSFIGDEPFAVLLGDDIVDSDVPCLKQLIDTYNEYKTTVLGV
                     QKIAKEDTNKYGILDVKHIEDRVYKVKDMVEKPAIEEAPSDIAILGRYIITPAIFSIL
                     EKQTPGKGGEIQLTDALQTLGKQEAIYAYNFEGRRYDVGDKIGFLEATIDFALKRENL
                     KDDLMDYMRKKVSEK"
     gene            7759..8772
                     /gene="galE"
                     /locus_tag="ACOIAF_001868"
     CDS             7759..8772
                     /gene="galE"
                     /locus_tag="ACOIAF_001868"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HGZ2668879.1"
                     /translation="MAVLVAGGAGYIGSHTAIELLESGYEVVIVDNLSNSNSIVVDRI
                     KELSKKPVKFYNIDIRNKDEMHVVFKENNIESIIHFAALKAVGESVEKPIEYYSNNLI
                     STLNLFELMREYGVKKFVFSSSATVYGDPHTCPILEDFPLSVTNPYGRTKLMIEQMLV
                     DISKADKSLDIALLRYFNPVGAHKSGRIGEEPNGVPSNLMPYITKIAVGKLKELSVYG
                     NDYPTHDGTGVRDYIHVLDLAAGHVKALQKLEENPGLVVYNLGTGKGYSVLDLVKAFS
                     KASGKEIPYKIVGRRAGDVAMCYADSSKAEKELGWKAKYELEEMCEDSWRWQSMNPNG
                     YEE"
     gene            8884..10818
                     /locus_tag="ACOIAF_001869"
     CDS             8884..10818
                     /locus_tag="ACOIAF_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp22"
                     /protein_id="HGZ2668880.1"
                     /translation="MKKSLKKYLVLALTLVLVFACGVPESSAASKHVIIVNSRKNTLG
                     YFVNNKLVKEFRVATGKKGSETPTGKTKVVNKIKNRPYYKGNIPGGSPRNPLGDRWMG
                     LALKGTYGDTYGIHGNNNESSIGKHISGGCIRMHNKDVRWLFDQVPVGSDVIIDYSND
                     SYVKIAAKYKINLNQTGWKTENGKKYYVKSDGTYQKNSWLKVNGKMYYFDASGVMQTG
                     WKTINNKKYYLGTDGARVSGWKVIDGKTYYFNSDGVMQTGWQEKNGKKYYLGSDGLAV
                     TGWQEIDGNKYYFDKTGIMQTGWQQIDGKSYYLDKDGKMLTGSQKIDGKDYTFNEDGT
                     INPTWDTIIGANRFDTAKKISSVGNWNADSSDTVILVNGNAIADGITATPLASSYDST
                     ILLTNTANLPTETVEEMKLLAPKTVILIGGENAISSKLEQEIKTTFNTETKRIAGQDR
                     YQTATRIAEELGNREEIKTAYMVSGNGEADALSVASKAGEEKQPIILVNKDGITEESY
                     KWLTERKLENAYFIGGPSAINDSVIAKMNDITTEDISGNRIYGDSRVDTNAKVIEKFY
                     GDTDLQAVLVSKSDALVDALSAGPLAVKLHSPIVLMDNSGLSSEQQRVFANKKVETPY
                     QIGGGVSYIVMDKLMDILAK"
     gene            10997..11836
                     /locus_tag="ACOIAF_001870"
     CDS             10997..11836
                     /locus_tag="ACOIAF_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HGZ2668881.1"
                     /translation="MSKIIKKKYIMFIDESGRADINHEDPFTLTGVIFEYKYAVSQGD
                     YVCSLRKEMDAFKEYCFGTSNIGIHLTDISRKSRAFEKFDDSQIKLFYDELPLFLSRL
                     ECTIISVTVDKARLKKYYAPSKEPYVVAFIHVLQNFYSFVNNETVESARIVIESRDDV
                     SNLKVQKAFFDVFNNGTIYLDIDEELRDKVKGFIIAKKEDADYREGLEIADILCNPLS
                     RARRGLIEANPKCMQYGEKNKIFKSVRNKIYTPTSVNDIRNWGFKEVPIVEPVGPWLE
                     KMR"
     gene            complement(12044..12925)
                     /locus_tag="ACOIAF_001871"
     CDS             complement(12044..12925)
                     /locus_tag="ACOIAF_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGZ2668882.1"
                     /translation="MNLYHLRYFVTLAHLEHYTKAAENLSITQPSLSHAISLLENELG
                     VALFEKEGRNIVLTKYGKIFLKDVEKSLEILDSSVKSLKITGTGEGQIDLAFLRTLGT
                     DFIPDIVHKFLKSNPAKSIDFKFHTGVTTDIIQGLKERKYDIAFCSKLEKEKGIEFIP
                     VAKQDLVLIVPYSHPLAAKDTIDLKETIPYPQIVFNQRSGLRYIIDDMFKKINQQPNI
                     VYEVEEDQVIAGLVAKNFGIAVVPNMNMLSFTKVKVIQIIHPSWERNFYLAFIKDRYL
                     PPAIKNFKNFVIKNAQL"
     gene            13338..14186
                     /locus_tag="ACOIAF_001872"
     CDS             13338..14186
                     /locus_tag="ACOIAF_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HGZ2668883.1"
                     /translation="MNNQKNLNIPITISSYTLGTEVSFRDRVRIAKEAGFDGIGLRAE
                     NYIDAKNAGVTDEEMIAILNEYDMKVTEVEYITQWGTEEDRTEAQREKEQNVYHMAHL
                     FNVKHINCGLLEMIPEEQIITALGELCDRAGELIIGLEFMPYSGVPDLATAWRVVKGC
                     NRENAMLILDTWHWARAKQTAEDLAPVPADKIVSIQVCDVLETPYEKLRDESLHDRLA
                     PGEGYGNTEEFVKIIKEHNISPRVIGAEVISDSLISKGLSTAAETVFNATKKVLDKAW
                     PEVSPK"
     gene            14265..16187
                     /locus_tag="ACOIAF_001873"
     CDS             14265..16187
                     /locus_tag="ACOIAF_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HGZ2668884.1"
                     /translation="MKISSMFTPIRIGSMTVPNRFVVPPMGNNFANTDGTLSETSKAY
                     YLERALGGFGLITIESSVVDKKAKGGPRKPCLYDDSTIDSFKNVIDACHDAGSKVSIQ
                     LQHAGSEGNEKVAGHPLKAASAIPASNGRNTPLAITTEEIYELIESYGDAALRAQKAG
                     ADAVEVHCAHGYLVSSFISQRTNKRVDEFGGCFENRMRLPRLIIENIRKKVGNSLAIL
                     CRINSTDDVPGGIDVHDSSVIAAYLEDCGIDGLHVSRSIHIHDEYMWAPTTLHAGFSA
                     ELVTEIKKAVSIPVITVGRYTEPQFAELMVRQGRCDLVAFGRQSLADPEMPNKAKNGK
                     LDEMIPCIACLQGCVPNMFQGKPIACLANPILGHEAELKPAEISKEVLVVGGGVGGML
                     AAWVCAKRGHNVTLVEKSEVLGGQMRLAAYPPGKGDITNLVRSYISKCNQYGVKICTN
                     TEATVELIKEKSPDVVIIATGATPLVLPIPGINDSGLIHAVDLLDGKKSCGKKVLVVG
                     GGMVGCEVAAFLGEQEHEVTVIELREEVGADVISEHRKFLMNDFENYNIHTITGAKVS
                     KFYDDGVDYLLTDGTEHDLKGFDSVVLAMGSRNYDPLSEVIKEVVKETYIVGDAVKAR
                     RALDATKEAFEVALNI"
     gene            16269..17132
                     /gene="aroE"
                     /locus_tag="ACOIAF_001874"
     CDS             16269..17132
                     /gene="aroE"
                     /locus_tag="ACOIAF_001874"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HGZ2668885.1"
                     /translation="MEISGRTGLFALIGTPVGHSKSPVMYNYSFKKLDLDYRYLAFDI
                     TVDKVKEALLAIKTFNIKGANVTMPCKSAVTEYMDELSPAARIIGACNTIVNDNGKLV
                     GHITDGVGYVRNLKENGVEVKGKKITIMGAGGAATAIQVQCALDGAREISIFNPKDDF
                     YKRAEQTVENIKKDVPECVVNLYDLEDTNKLYEEIQSSDILTNATLIGMKPYDNETNI
                     KDTSVLRKDLVVTDVVYNPKKTKMIEDAEANGCKAIGGLGMLLYQGAEAFNLYTGLEM
                     PVEEVNELCFK"
     gene            17332..18549
                     /locus_tag="ACOIAF_001875"
     CDS             17332..18549
                     /locus_tag="ACOIAF_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGZ2668886.1"
                     /translation="MNNKKYYPTAIALYFSYFLLGIGISILGQYKPEFSSMWGAKTLS
                     DGTLDVSIVLAVIAALGLGRLISYPFAGPISDKYGRKVSGLIGNFLHAIFFVGIVFSP
                     NFYIAYVFAIIGGAANSFLDTCVTPSCMEIFASLGTIANMFTKFTIALAQFLLPFIIG
                     FVAANSISFKVIFIVTAILIVVDAFLIAVLPFPPANNVIDNKGKTVKSEKMKFTPTSI
                     ALVCIGFTCTSTFVLWLNCNQELGKLYGMADPTKIQSFYSMGVICAVLITSLLIKKYI
                     KPIRILVIYPIIALLMLLVVYFVQTPTICMLGGFVIGYSAAGGVLQLTTSTANEMFPT
                     NKGKITSIVMIASSIANYVILNIAGIITKSGGVNGPKYVVLFNVAITFVGILLALFVN
                     MRYEKEKVYDYDV"
     gene            18785..20650
                     /locus_tag="ACOIAF_001876"
     CDS             18785..20650
                     /locus_tag="ACOIAF_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HGZ2668887.1"
                     /translation="MENVFKRLQEFNGYDGYKESFEMNYLCIYESIPLREQVELANNL
                     VDEILNMYKSESNEIYLLEDSNSKSLICYFEIFMKKINTLVKEMIIDEKWLYKLTKEL
                     IYKSKKVEYVKLGLVLSEKYLNVENLREVVDTFSKSGEYVFYLSNTIKKLEFYNTYLF
                     NLSKKATGSIKVFAIVNMENLDSKINSYLIEDGYKDTKYERLLMNYIISIVDLNEYLE
                     KRDLDKEKINNLARLICNYLLSVEFKYIGNKLELVNRFLPTVVNYGTNFESLYSIFLI
                     AINVLKDENIECNKIEFEKEINDILLSEKWKNIYFEALRDASGKTEDIIKMSEIYDVN
                     LSFDDLLPYLNRDIRDFEVYWHISKKGTTSSRLKLLNFFEETFKIDDLIGKMKDIEKD
                     KLTQEYYDDMLFFIVLKGSKSLYPEGKNISLKGIFGNINEVRKESINILKRYREKLSL
                     EELKIVKEAYEKEKNVILKDELRRVLYESNNLKKEFVNIEKIKVDEHGKDIYLTSIAV
                     AGSRFRNREYLEKELEKSKIYYLTREKDNLYDEKAIKIVGETGYVIGYVPRKENYILS
                     NLLDGGKLLYCRVTEYNLYEDCIYANVYLSYKDVIETVENSLKMVLDKSRIKLIN"
     gene            complement(20828..22192)
                     /locus_tag="ACOIAF_001877"
     CDS             complement(20828..22192)
                     /locus_tag="ACOIAF_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HGZ2668888.1"
                     /translation="MSQLLIKNAYLITMNADREVYETGSILIENNIIKNVGKFDINIV
                     DKDAEIYDAKGKILMPGLVNTHVHLSQQLGRGLADDVVLLTWLRERIWPYESSFNYDD
                     SLISSTACCVELIKSGVTTFLESGGQYVDAMVEAVDKTGLRACLAKSVMDTGDGLPEA
                     WQKTTDEEINTQLDLFHKFNNTSNERIKIWFALRTIFNTTEELLVKTKELADKYNTGI
                     HMHIAEIADEISFVKQNNGVGTVEYLDKLGVLGPNLLAAHTVWLTHKEIDLFRLHDVK
                     VSHNPAAAMKVVLGFASIPEMLEKGIPVSIGTDGAPSNNRMDLMRDMYLTSLIHKGRT
                     LNPTVVPAEEVLEMATINGAKCALLEKEIGSLEVGKKADMIILNPDTIHCLPMHNPIG
                     NIVYSMTSENVDSTICDGKWLMKERKVLVVDESELLNKVKKQASKIKDKAEVKIPSKF
                     KIIK"
     gene            complement(22371..23714)
                     /locus_tag="ACOIAF_001878"
     CDS             complement(22371..23714)
                     /locus_tag="ACOIAF_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HGZ2668889.1"
                     /translation="MVPNTNNVQPGGNNSSLSFLDKMFKFSERGSSLKTEVIGGVTTF
                     LTMAYIVFVNPAILSEAGMDKAALITVTILATAIGTLIFAILGNAPFALAPGMGLNAF
                     FTYSLVIGAGIPWQTGLGVVFLSGCVFFTLAVTGLRKTIADAIPRELAVASSAGIGLF
                     LAFVGLKNMGVIIANPDTIVSLADFTPTVLIALFALILMAILEYKKVKGGILISIVVA
                     TIISVIFGFVELPTAIISTPPSIAPVAFKLDIVGALKFSLIGPIFSFMFIDMFDSLAF
                     LITCCKQMGLEDENGEIKDLGRMLYADVSSTLIGSFLGTSTVTTFGESASGIAAGART
                     GLASIVTAALFLATLIFTPLLGIVPTYAAAPALVMVGVFMFESIKQIDFADSKIAVPA
                     FVTVLLMPLTYSISIGLCFGFISYIIMHVVAKETDKISITLWIIGLLAVVNLVLS"
     regulatory      complement(23787..23888)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            24217..24870
                     /locus_tag="ACOIAF_001879"
     CDS             24217..24870
                     /locus_tag="ACOIAF_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HGZ2668890.1"
                     /translation="MTKIIKVQKPKFTGELEIVENLEDILEDIFNDEKIFNKIIENEV
                     ISDLDSVRVSFESCIFRNVSFENCNLKKIDLLDVVFENCNLSNTFFDGGSIYRVEFKN
                     SKLIGVRFDDCILKNVLFKDVLGKYSNFSFSKFKIVSILDSNFENSVFQEVKGDNLVF
                     EYTNLKKANFNGTKLSGIDFTTNNIEGIEIGIDDIRGAIFDVSQAIDLTKLMGIIIK"
     gene            25011..26282
                     /gene="brnQ"
                     /locus_tag="ACOIAF_001880"
     CDS             25011..26282
                     /gene="brnQ"
                     /locus_tag="ACOIAF_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HGZ2668891.1"
                     /translation="MRDIFIVGFALFSMFFGAGNLMFPPFLGMESGKDWLIPLIGFVV
                     ADAGIAMLVIIATARCKGSMDDVLRRAGKGLARVMSVAALACLALLVIPRTCATTYEM
                     GIMPIFGDHGPVAKAVFSIIFFGLTLAFTIRSSKVIDIIGKYLTPALLLVLFVLIVKG
                     IISPAGPMSPEHMIDKNLFGEGISQGYQTMDALGAASMATIMLMSIIAKGYTSDKDQI
                     GMTIKAGFVACVFLAVVYGGLTYLGATVSTLYDTSIPQASLLVEITSILLYGQVGKVI
                     LGVIVALACLTTSTGLTASISTYFEGISKASYKQLVIGICVASMLISNLGVDSIIAIS
                     VPILQTIYPVLLAIVVMELAGKHIKNDNAFKGAAYVTLVISLLSAINGMTGAVPFIQS
                     LPLAGLGFNWVIPAILGGIVGNFIKSNKQVA"
     gene            26484..27671
                     /locus_tag="ACOIAF_001881"
     CDS             26484..27671
                     /locus_tag="ACOIAF_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HGZ2668892.1"
                     /translation="MLRKITAFMLSICLIIGLTGCSLLVGKKDKEKIIDDFKVAVVTQ
                     PLSENKVQYNMVEEMAKEYEEENKIDKDKDGQTKVKQTIKHVVLPENFTSNIDSAINK
                     IVKLADDKEVQAIVVSTDQAGLLPALQKVKEKRPEIITISAPMGDDKNQLSQFVDVNL
                     GVSAEERGKVLAERSKEMGAKAFIHYASTDDLKDVNIAKRLEMIKETCKNIGLPFVQV
                     NTPNINTEEDKSKVKQFLNEDIEKQVKKYGKDINVFGVNEYMDEVILTKALELKYIVA
                     EQSNPSPIQTYPSVMGLKISEKDAQNYDKINDMISEKAKAFGMSNRLGGYPMPMDAFL
                     PSVAIYLATEMVKQDLTQEDVCDPDYLEAFTELRFGIGSEFTPLTEVLYNYQSVILSQ
                     LIY"
BASE COUNT        10851 a         3106 c         4837 g         8939 t
ORIGIN      
        1 taacttttaa aaattagttt tactaattta caataaaata ggtgtgctgg tgtggctcaa
       61 cggtagagca gctgacttgt aatcagcagg ttgtaggttc gattcctatc accagctcca
      121 gtaaagacta tgtttttagc atagtctttt tttattgttt tttattgttt gatgtatttg
      181 ttattttgta ttaaatactt ttagtggtct aatttataaa ttataggtat aaaagatgac
      241 acagtttaaa attattgttt taaactgtgt ctgtgagttt taaatcaatc taccatgaat
      301 caccattctg gcatcatcaa attcataggt acatgtagaa agtgtaacta ttttatcatt
      361 gctatttacc ttaataggag atttatacaa tgatttagaa gtaatatcat ttatgtaatt
      421 ttgataatca gattcattgt taaaatttgt ttttagataa tcataatcag attctacaat
      481 ataagcagaa aaaacatcat ataaaaattc ttttccattc aaggttattt ttattttatt
      541 atttttttta aagaagtcag catctttaaa cttatttagg ttgttgaaca tagttttatt
      601 tttcatatta tgtccatata ttatgatatt tttgtcatca atagatttat ttctataatc
      661 cataaacagt gtaccagaaa tagaatcttt tttataaaaa tctttgtcaa gatagtaaga
      721 attatcttta gattgtacaa ctggataatt tatatttgta ttctcaacag aaagccaaaa
      781 tttaaaatca ctgttaatct ttgataaatc ctctttttta tattcttttt tttgaagttc
      841 tgaggatatt ttagtatcat gattgtattt ggtaagtttt gagtatatat taaatccact
      901 gtaaagtatt actaatacta gtataatgtt aattactatt ctatataatt ttttcaatat
      961 atcacctcga gttaaatgta gtttatatat ctatattaca ctatttttga ataaatttaa
     1021 atttttagta taatattact ttgacatgta aaagaatttg tgttaacata actaagtaaa
     1081 ataaatatta agtaagccag ataatcgcat gtaatcttac atgaggaaag tcggagctcc
     1141 atagagcagg gtgctaggta acgcctagtg ggagcgatcc taaggatagt gcaacagaaa
     1201 taaaccgcca ctattgtggt aaggatggaa aggcgaggta agagctcacc agcagctagg
     1261 cgactagttg gctatgtaaa ccccacctgg agcaagacca agataggaat aaagagtgct
     1321 gctcgtactc tccggtaggt aggtcgcttg agcctattgg tgacaatagg cctagaaaga
     1381 tgattatcta atacagaact ccgcttatag acttgcttaa ttatttcgat agtcaaaaac
     1441 atcagtttaa actgatgttt ttttatttgt aaatttgtct aatctacttt taaaattttg
     1501 aggaatattg taatataagt tagtacagat attaaaaatg gtataatata agatagatat
     1561 aaaaagaaaa agtaaaatga tgtaaataat atgatatgat ataagatgta gcaaatttag
     1621 taaataatta tttagatatc ataatcaaat tagcaacttt gcacaatgta aatattacaa
     1681 taaaataatt gatttaagga gtgaaggttt tgagtgaaaa taatataata agaaacactg
     1741 ttttaaatgt cacagcaagt tttttaaaac aagagtctaa aataaatgaa aagctagatg
     1801 gtgttttgga aaagaaattt gaaaaagtag aatttaatga agcaaaatat gcagagcttt
     1861 taaaatttaa tatattgttt tataagacat tagcaagaaa tacagaacca cttattggca
     1921 aatggatagt agataagtac atacctgaaa tagatgaatt agaaaaagat ttagaattaa
     1981 ctacagcaaa atgtagaaag tatgtaaata aggctatgaa agagggatta gactctctaa
     2041 aagccaatga tttaaatagt tttttagctt atgataaaat ggaactatca gagagaagaa
     2101 gacgcttaga aaaggattat aaggttttaa acctatataa agaccttctt aatatatctc
     2161 ttagaaaaat aagtttagaa aagaaagaat gtggagattt atttcttaaa aatcaagcag
     2221 atgcgaaaag agaattaaaa agagaaatta tattctgtgt aaacaaaata ttagctagca
     2281 ataaagatgt ggtacctaat aatgtggaag agaataacga acaagacaaa gtagaaataa
     2341 aagcaactga aattgaggat ttgcaattag gtaaagataa ttcagaaaat gacatgctac
     2401 taaatgaaat taaaaattca aaagtaaaac atgaatcaaa atcaaatgat atagaatcga
     2461 agtttataga taagttaaat agcttaaata atgaaagtgt atctcaaaca atacacaatg
     2521 aatataagaa gttatgtgga ttagaagaac ttcattctgt tgaaggatat ggttttggca
     2581 aagaaattat taaagatttt gcttgtgcaa cggttgtatt agaattttta aagagaagaa
     2641 atagagacct tatagaaggt gcaatgagac tgactataat tggagaattt ggtcctgaaa
     2701 attttaagga atttgtggac tatgtaatta aaaataaaac agaaattagt gaagatacat
     2761 ggaataaagc acagaatcta ataaaagata actatagtga attagaaaat catgaaattg
     2821 catcaaaaag aactagaaga aataaagata tcgatattga agaatacatt tacatgataa
     2881 aaaatgcaga taaagatatt tgctttagaa gtagtatttc aatagaagat gatgctgaag
     2941 aagggactaa agaagaagta gatagtaata atcaagatat aggtgacgtt gtagaagata
     3001 aagatactac agataaagag tacgacagca ataaagaaga tataattgaa ccagaaaata
     3061 aaaagagtaa aaagaaggct aaattatttg gatttattaa aaaagacaat gaagaagttg
     3121 aacaagaaga agagaattta aatgatattt ctccagatat aattttagat aaaccagttg
     3181 aaaatcatca agtgaaatca gaagaaatag agcaaaatga attaaaagaa ataaaacaag
     3241 aagaaccaag tcaacatata gaagaagaac gtagtgtaaa aatagaaaaa ccaataaata
     3301 ataacttaga tgaaaaagta tcttcaaata atgaatcgaa gcttgaaaaa gaaagtaaaa
     3361 acttagaaga taaaaaagca aaagaaataa aagaagaaaa attagaaaat gaaaaatcag
     3421 tagtaatacc tattaaaaag aaagaaaata gtaataaaaa atctaaaaat tcttctaagg
     3481 acaaatatag agaaaacaag aaagaaatga aaaattatgt atcagataaa gaagattctt
     3541 tagatgatga agaggttgtt tctaagaaga gtagattaaa agaaactatt atagcagtag
     3601 ttatagtggt tattgttggt gtaggatatt ttataacagt aggcaacaat aagaaaaatg
     3661 ataaagagaa tattccaaag tcatcaactc aacaacaagc taacaataaa ttaactgagg
     3721 aagagaaaaa agcacaagct gaaaaagaga aaaaagaagc agaagaaaag gcaaaagctg
     3781 aaaaagaggc tcaagaaaaa gcacaagctg aaaagaaagc aaaagaaatg gaagcatata
     3841 aagatggtaa aggtgtatat tacactgttt atgcaggttc acttaaagtt gaaaaaacag
     3901 caaaagaaac agcaaaagaa tatgaggcta aaggtatttc ttcaacaata attcaagaaa
     3961 atggatacta taaaataaaa ataggtgatt attctcaata tggagaagca caagaaaaat
     4021 gtaatgaact agctaagaag tctattgata catatatagc tatgtatgat aagtactatg
     4081 attataagtt agaagaatta aaagaatcag ctccttcttt gagtgctgaa gaacttaaac
     4141 aaaaatatga agatttaaga agtgagttaa agaacaaatc tggatatcgt gaatatgtta
     4201 aacatttaga taaactttat gaagaaatag ttgaaggtgc ttagagcatt ataaaaaagg
     4261 agaaactatg tctcagaata aaaagtataa aaaaaaatat tatttgtttt gagacatttt
     4321 attcaccttt ataaaatact tataattctg tttaggtggt gaaggtatgt tgaaattagg
     4381 agaaaaaatc atatatgagc tttcagatgg gtttgagctt agtggcataa gtagatactc
     4441 aaaagaatat aaaaagcttc atataacaaa tctagggaat ataataatca gcaaatcaga
     4501 tgaagttaat gaggatacag gtgtaaaata tatatatagt ttttcagaca ataaagaaaa
     4561 attagaggca gttttagaag attctcaaat aattgtctat ggaaataact tgccttttta
     4621 cataaaaagt gaaaattcaa gtaaaaatgt tacagcaaat ttgactatga aaataacttt
     4681 ggatgaatat gatttaatct gcaaaagtca aagagaattt atattctatc taaaggataa
     4741 tatgactata ttaagtttag atgacaataa gttttatatg ggtggaatta ataaagataa
     4801 tgagaagttt atatttatca gtgggaaaaa tagatttgaa ataaattttg atgatataga
     4861 aagatacatt ttagaagata aaagagtgtc attgaaggga tactttcata tggaaagaga
     4921 ggggattata gtaaggtctg tatctatatt taataacaat atagatagtg tagtcccatc
     4981 tgatttaaat gagagagtta aggataacca aaaaatagga aatcttccta aagattgtga
     5041 aatagtattc tgtaagatat caggaaatat agatggattt gattataaaa acacaaacat
     5101 gcttttagta aaatatcaag accaattaat ttttataaat aagaaatcta agaagactat
     5161 tgtaaagtca gcgaaagata attgttctaa gttaaattta ggagaagata taatattata
     5221 tgataataaa aatgtattta acctacatat aaatgataaa aatagagaaa ttatgcaaat
     5281 agataaccta aaagatatag aaaatgaaat agtgggatat acattaaaac atgctccatt
     5341 ttttattcaa gaagatttcg atagcttgac tattctaaag tcttttcaaa aagaaatcat
     5401 atctataaaa aatagtgata taaaagacat tgtaattaat aaagaacttg aaaacgaaaa
     5461 ttctaatttt gtagaaacgg aaataaaatt taataatcaa aaagtacttt taaacttaag
     5521 taaaagtatg gttcaaaaac ttatgcaaga tgtatttata tatgcaaagc aacctctact
     5581 taaagaaaat tctattgaag tgatatataa gaattggtct aaagctatga atgatatgat
     5641 aatatttaat ttctttggaa atatttacta tatgaaatct gaatttgata agattttaga
     5701 aaaagagtta aatgatgaaa taagaataga agttataaat tcactttata agcaaataca
     5761 agaacaaaga aacaatttag atttattgtc agcatatatg ccaagaatat tagaaaatca
     5821 agaaatagat ttgtttgaaa agtataatac taaacttgat gtacaagtgt ttaagcagat
     5881 taaaaatcta ttgtctgact tatcatataa tataagcagt tatttaaatg aagttgaaaa
     5941 aagtttagac aatatcatct ttgtaatttc aggagaagat aagaagaaat acaattatag
     6001 aatgttaaaa gaaagtgaaa gtgcaagtct tgatgtattt ttaaaacaag ccatatcaag
     6061 attaaaccat ttagtagaaa atatgtatcc ttattatgta gatgaaacta gtagggaaat
     6121 gtttaagtta tttgagttat tgtggaaaaa ctatagaaat attgatgatg atagtataaa
     6181 agaaatatta ttcgagagaa taacaaatac atatgtattt aaacaattga cattaaataa
     6241 tagtacaaaa gaaagaagaa aagacataat tgaaaaaata tataactctg ttgattatgg
     6301 aaccaataaa ctagatgaaa atatgttttt cacaggagga ataaagtatg tcaagtaaat
     6361 acaatgtttg gacttttaac tatgagttct taggattgga ctcaggggat gaaaaaaata
     6421 atgaagttta ttacgagatt gatttagatg aaattttaaa tgaaaactat atagatgaag
     6481 atatagatag tggtttagat tctgatggaa atagtatttt aggagaatta atatcagaaa
     6541 tggatataga tgaggatgtc agtgttgata tgacatatca agaactagaa gttgacttag
     6601 aagatttaga ttcttatcta gatgaagata tagatagtga tataaaaggc atcttagatg
     6661 aaatttagta cctgtataag ttttggataa aatataaaca atacaatatt aacttataaa
     6721 actaaaggta gatgaaattt catctacctt atattatctt atagcattaa atattgggat
     6781 aatatagagt ttgaagaaaa agtataaata tggtatatta atttggtaca atagaaaatt
     6841 tacgaagaag ggtgaaaaaa tgcagatgac agttaaaaaa gcaataatac cagcagcagg
     6901 gctaggaact agatttttac cagcaacaaa atcacagccc aaagaaatgc ttcctatagt
     6961 agataaacca actttacagt atataataga agaagctata gaatcgggaa ttgaagaaat
     7021 tcttataata acaggtagaa ataaaaaatc tatagaagat cattttgata aatcagttga
     7081 attagagtta gaactagagc aaaaaggtaa gacagagatg ttagaaatgg taagagacat
     7141 atcaaatatg gtaaatattc attatataag acaaaaagag ccaaaaggtc ttggacatgc
     7201 aatttactgt gcaaaaagtt ttataggtga tgaacctttt gccgtattat taggtgatga
     7261 tatagtagat tcagatgttc cgtgcttaaa acaattaata gatacttata atgagtataa
     7321 aacaactgta ttaggtgttc aaaagatagc taaagaagat actaataaat atggtatatt
     7381 ggatgtaaaa catatcgaag atagagtata taaagttaaa gatatggttg aaaaacctgc
     7441 aattgaagaa gctccatctg atatagctat acttggaaga tatataataa ctcctgcaat
     7501 atttagcata ttagagaaac agacacctgg taaaggtgga gagatacaac tgacagatgc
     7561 acttcaaact cttggaaaac aagaggctat ttatgcttat aactttgaag gtagaagata
     7621 tgatgtggga gataagattg gttttttaga ggcaacaata gactttgctc taaagagaga
     7681 aaatttaaaa gatgatttaa tggactatat gagaaaaaaa gtaagtgaaa aataaatatt
     7741 agaaaagagg taattacaat ggcagtttta gtagctggag gagcaggata tataggaagc
     7801 catacagcaa tagaactttt agaatcaggt tatgaagttg ttatagttga taacttaagt
     7861 aatagcaact caatagtggt tgatagaatc aaggaattat ctaaaaaacc agtaaaattt
     7921 tataatattg atatcagaaa taaagatgaa atgcatgttg tatttaaaga aaataatatt
     7981 gaatcaatta tacactttgc agctttaaaa gcagttggtg aatcagtaga aaaaccaatt
     8041 gaatattaca gcaataatct aataagtacc ttaaatttat ttgaattaat gagagaatat
     8101 ggtgttaaaa agtttgtatt tagttcttca gctactgtat atggagaccc acatacttgt
     8161 cctatattag aggattttcc actttctgta accaatccat atggaagaac taaacttatg
     8221 atagagcaga tgttagtaga tatatcaaaa gcagataagt cattggatat agctctttta
     8281 agatacttta atccagtcgg tgctcataaa agtggtagaa taggtgaaga accaaatgga
     8341 gttccgagca atctaatgcc ttatataaca aaaatagcag taggtaaatt gaaggaattg
     8401 agtgtttatg gtaatgacta tccaacacat gatggaactg gagttagaga ctatatacat
     8461 gtattagatt tagctgctgg tcatgtaaaa gcattacaaa aattagaaga aaatccagga
     8521 cttgttgttt ataatttagg aactggaaag gggtatagtg ttcttgattt agtaaaagca
     8581 ttttctaaag caagtggaaa agaaatacca tataagattg taggaagaag ggctggagat
     8641 gtagccatgt gttatgcaga ttcttcaaag gcagaaaaag agcttggatg gaaagctaag
     8701 tatgaacttg aagagatgtg tgaagattca tggagatggc aaagtatgaa tcctaatggg
     8761 tatgaagagt agaatacata cttatatgta taaatgtaac aaatttgtaa atataaggta
     8821 ttggtaattt tatgttataa ttagacacat gtagtaatgt ttctaaaaga gggggaagtt
     8881 atagtgaaaa aaagtctaaa aaagtattta gtactagcac tgactttagt tttagtattt
     8941 gcatgtggcg taccagaatc aagcgcagcc agtaaacatg tgattatagt aaattcaaga
     9001 aaaaatactc taggatattt tgtaaataat aaacttgtaa aagaatttag agttgcaaca
     9061 ggtaaaaagg ggtcagaaac accaacaggg aaaacaaaag tagttaacaa gataaaaaat
     9121 agaccatatt ataaaggtaa tatacctgga ggaagtccaa gaaatccatt aggagataga
     9181 tggatgggat tagcattaaa aggaacttat ggagatacat atggaataca tggaaataat
     9241 aatgagagtt ctataggaaa gcatatttct ggtggatgta taagaatgca caataaagat
     9301 gttagatggt tatttgacca agttcctgta ggttcagatg taattataga ttatagtaac
     9361 gacagctatg taaagatagc ggcaaaatat aagataaatc taaatcaaac aggatggaaa
     9421 acagaaaatg gtaagaagta ttatgtaaaa tctgatggaa cataccaaaa gaatagttgg
     9481 ctaaaagtaa atggaaaaat gtattacttt gatgcttcag gagtaatgca aacaggatgg
     9541 aaaacaatta ataacaaaaa atattattta ggaactgatg gagcaagagt atctggatgg
     9601 aaagtaatag acggaaaaac ttattacttt aactcagatg gagtaatgca aacaggatgg
     9661 caagaaaaga atggaaagaa atactatcta ggatctgatg gtttagcagt aacaggatgg
     9721 caagaaatag atggtaataa gtattacttt gacaaaacag gaataatgca gacaggatgg
     9781 caacaaatag atggtaagtc ttactattta gacaaagatg gaaagatgct aactggttct
     9841 caaaaaatag atgggaaaga ttatactttt aatgaagatg gaactataaa tcctacatgg
     9901 gatacaatta taggtgcaaa tagatttgat acagctaaaa agataagttc agttggaaac
     9961 tggaatgctg atagttcaga tactgtaata ttagtaaatg gtaatgcaat agctgatggt
    10021 ataacagcaa ctcctttagc aagtagctat gactcaacaa tactacttac taatacagct
    10081 aatttaccaa ctgaaactgt tgaagaaatg aagttattag cacctaaaac agtaattttg
    10141 ataggtggag aaaatgcaat aagttctaaa ttagagcaag aaataaaaac tactttcaac
    10201 acagaaacaa agagaatagc aggtcaagat agatatcaaa ctgctacaag aatagctgaa
    10261 gaacttggaa atcgagaaga aataaaaact gcatatatgg tttctggaaa tggggaagca
    10321 gatgctttat ctgtagcttc taaagctggg gaagaaaaac aacctataat attagtcaat
    10381 aaagatggaa ttacagaaga atcttataaa tggctaacag aaagaaaatt agaaaatgca
    10441 tactttatag gtggaccaag tgcaattaac gattcagtaa tagcaaaaat gaatgatata
    10501 actactgaag atatttcagg aaatagaata tatggagata gtagagtaga tactaatgcc
    10561 aaagtaatag aaaaattcta tggagatact gatttacagg ctgtattagt tagtaaatca
    10621 gatgctttag tagatgcttt atcagctgga ccattagctg taaaattaca ttcaccaata
    10681 gttttaatgg ataattcagg attatcatca gagcaacaaa gagtatttgc aaataaaaaa
    10741 gttgaaactc cttatcaaat agggggcgga gtatcttata tagtaatgga taaattaatg
    10801 gatattcttg caaaatagta aaaacatata ttgaaaaata attataattg agatgtgaaa
    10861 ttcaatcaat aaaagcttga ttgttttaca tctcaatttt ttagtataaa attaagttta
    10921 agttatataa aaatcttgga atatttttaa aactcatata aaatgaatat agatgctatc
    10981 atcatatgtt aagagggtga gtaagattat aaagaaaaaa tatataatgt ttattgatga
    11041 aagtggaaga gctgatataa atcatgaaga cccatttaca ttgacaggag taatttttga
    11101 atataaatat gcagtaagtc aaggcgatta tgtatgttct ttgaggaaag aaatggatgc
    11161 gtttaaagag tattgttttg gaaccagtaa cataggaata catttgacag atatatcaag
    11221 aaaatcaaga gcatttgaga agtttgatga ttcacagatt aagttattct atgatgagtt
    11281 gcctttattt ctatctagat tagagtgtac tataatatca gtaacagtag ataaagctag
    11341 attaaagaaa tattatgcac cttcaaagga accgtatgtt gtggctttta tacatgttct
    11401 tcagaatttt tattcatttg taaataatga aactgtagaa tctgcacgaa tagttataga
    11461 gtcaagggat gatgtctcaa acttaaaagt acaaaaggcg ttttttgatg tatttaataa
    11521 tggaacaata tacttagata tagatgaaga attaagagat aaggttaagg gttttataat
    11581 tgccaaaaaa gaagatgctg actatagaga aggtttagaa atagcagata ttttatgtaa
    11641 tcctcttagc agagcaagaa gagggttaat agaagctaat ccaaagtgta tgcaatatgg
    11701 agaaaaaaac aaaatattca aatctgtcag aaacaaaata tatactccaa cgagtgtgaa
    11761 tgatataaga aattggggat ttaaagaagt tcctattgta gagcctgttg gaccatggct
    11821 tgaaaaaatg cgttagatac aattgacaaa ttttaaaaaa tctgatataa tgtaagtaac
    11881 ctagttattc aggaactgaa tccagttgct gttgtataac gataattact gagactcgga
    11941 tgccgactgt cagttaggaa tctatacttt taaaagtata gattttttta ttttataaaa
    12001 agaataaaag aggatattta aaaataaccc ctttattaat aaattataat tgagcgtttt
    12061 taatgacaaa gtttttaaag tttttaattg caggaggcaa atatctatct tttataaatg
    12121 ctaagtaaaa gtttctttcc caacttgggt gaataatctg aattactttt acttttgtaa
    12181 agctaagcat attcatattt gggactacag caattccgaa attttttgca actaatccag
    12241 caattacttg gtcttcctct acttcataga ctatattagg ctgttgattt atttttttaa
    12301 acatatcatc aattatatat ctaagaccgc ttctctgatt aaatactatt tgaggataag
    12361 gtatggtttc ttttaaatct atagtatcct ttgcggctag tggatgacta tatggtacaa
    12421 ttagtactaa atcttgtttg gcaacaggta taaattcaat tcccttttct ttttcaagtt
    12481 tcgaacaaaa tgcaatatca tattttcttt ctttaagacc ttgaataatg tcagtagtta
    12541 caccagtatg aaatttaaaa tctattgatt tagcaggatt agattttaaa aatttatgca
    12601 ctatgtctgg gataaaatca gtaccaagtg ttctaagaaa agctaagtct atttgtcctt
    12661 caccagtacc agttattttt aatgatttta cacttgaatc taaaatttct aatgattttt
    12721 ccacatcttt taaaaatatt tttccatact tagtaaggac tatatttcta ccttcttttt
    12781 caaatagagc caccccaagt tcattttcta gtaaagaaat agcatgactt aagcttggtt
    12841 gtgttataga taaattttct gccgcttttg tatagtgttc taaatgtgcc aatgtaacaa
    12901 aatagcgtaa gtgatataaa ttcaattttt gttcatcctt tcataggtat ttttatttat
    12961 atatttgtag aagttaacct ttaagatatt tttacatttt tattatatat gattatatag
    13021 taagaataaa agcttttaaa aaagtttatc gttttttgtc tgaattataa gataaatata
    13081 tttatacgac ctaatagaaa aatacaagaa ttatcttata aataaaatct ataaacaaaa
    13141 tctatgaatt aataggaaat tatcaatttg ttattagtaa agtctcgtga tacaataaat
    13201 ttataaaatc tagataaata gaaatcaaaa aatactttta tttttaaggc aaaataatac
    13261 tactagtagg ataaataaaa atataaaatc aaaaatacta ttaattataa agaaaataaa
    13321 gggggcaaat ttaagctatg aataatcaaa aaaatcttaa tattccaatt acaattagtt
    13381 cttatacatt gggaacagaa gtttcattcc gtgatagagt tcgtattgca aaagaagctg
    13441 ggtttgatgg tattggattg cgtgctgaaa attatattga tgcaaaaaat gcaggtgtta
    13501 cagatgaaga aatgattgca attcttaatg aatatgatat gaaagttaca gaagtagaat
    13561 atataactca atggggaaca gaagaagata gaacggaagc tcaaagagaa aaagagcaaa
    13621 atgtttatca tatggctcac ttatttaatg ttaaacatat aaattgtgga ttattagaga
    13681 tgataccaga agaacaaatt ataactgcat tgggagaatt atgtgacaga gcaggtgaac
    13741 ttattatagg tcttgagttt atgccatata gtggtgtacc agatttggct actgcatggc
    13801 gtgttgtgaa aggatgtaat cgtgaaaatg caatgcttat attagatact tggcactggg
    13861 ctagagcaaa gcagacagca gaggaccttg cacctgttcc agcagataag atagtatcaa
    13921 ttcaagtgtg tgatgtttta gaaactcctt atgaaaaact tagggatgaa tcactacatg
    13981 accgtttggc tccaggtgaa ggatatggaa acacagagga atttgtgaaa ataataaaag
    14041 aacacaatat atcaccaaga gtcatagggg cagaagtaat atctgattct ctaatatcta
    14101 aagggttatc cactgctgct gaaactgtat ttaatgcaac taaaaaagtt ttggataaag
    14161 catggcctga agtatctcca aaataaactt atataatata aaaatataca gagtattata
    14221 atataaaact tttaaatata aaacttgaaa ggaaggaaaa ccttatgaag attagttcta
    14281 tgtttacacc tataagaatt ggttcaatga cagttccaaa tcgttttgta gttcccccta
    14341 tgggaaacaa ttttgcaaac acagatggta ctttaagtga aacttctaag gcttattatc
    14401 ttgagcgtgc tttaggtggt tttggattaa taactataga atctagtgtt gtagacaaaa
    14461 aagcaaaagg aggtcctcgt aagccttgtt tatatgatga tagcacaata gatagtttta
    14521 aaaatgtaat agatgcttgt catgatgcag gttcgaaggt atctatacaa ttacaacatg
    14581 ctggttctga aggaaatgaa aaagtagcag gacatccatt aaaagctgct tctgcaatac
    14641 ctgcatcaaa tggtcgtaat acaccactag caataacaac agaagaaata tatgaactta
    14701 tagaatctta tggagatgct gcattacgtg cacaaaaagc tggtgcagat gctgttgaag
    14761 ttcactgcgc tcatggatat ctggtcagta gttttatatc acaaagaaca aataagcgtg
    14821 tagatgagtt tggtggatgt tttgaaaata gaatgagatt acctcgtctt atcattgaaa
    14881 atatacgtaa aaaagtagga aattctctag caatattatg tcgcataaac agtactgatg
    14941 atgttccagg aggaattgat gtacacgata gttcagtaat agcagcatat ctagaagatt
    15001 gtggaataga tggacttcat gtttcacgta gtatacatat acatgatgaa tatatgtggg
    15061 ctcctacgac tttacatgct ggattcagtg cagaattagt tactgaaata aaaaaagctg
    15121 ttagtatacc agtaataaca gttggacgtt atacagagcc acagtttgct gagttaatgg
    15181 taagacaagg aagatgtgat ttagtagcat tcggtagaca aagcttggct gatccagaaa
    15241 tgcctaataa agctaaaaat ggaaaactag acgaaatgat accatgtatt gcatgcttac
    15301 aaggatgtgt accaaatatg ttccaaggca aacctatagc ctgtcttgca aatcctatat
    15361 taggacatga agcagagctt aagccagctg aaattagcaa agaagttctt gttgttggtg
    15421 gaggtgttgg aggtatgctt gcagcatggg tatgtgctaa aagaggacat aatgtaactt
    15481 tagttgaaaa atctgaagtt cttggtgggc aaatgcgttt agctgcatat cctccaggaa
    15541 aaggagatat tactaactta gttagaagtt atatttcaaa atgtaatcaa tatggtgtaa
    15601 aaatatgtac taatacagaa gctacagtag aacttataaa ggagaaatca cctgatgtag
    15661 ttattatagc tactggagca actccacttg tacttcctat accaggaatc aatgattcag
    15721 gattaattca tgctgtagac cttcttgatg gtaaaaaatc ttgtgggaaa aaagtacttg
    15781 ttgttggagg tggaatggtt ggatgtgaag tggcagcctt ccttggagaa caagaacatg
    15841 aggttactgt tattgagctt agagaagaag ttggtgcaga tgtaatttca gaacaccgta
    15901 aattccttat gaatgatttt gaaaattata atatacatac cattactggt gcaaaagttt
    15961 ctaaatttta tgatgatggt gtcgactatt tacttacaga tggcacagag catgatttaa
    16021 aaggttttga ttcagtagta cttgctatgg gttctcgtaa ttacgaccca ttaagtgaag
    16081 ttataaaaga agttgtaaaa gaaacttata ttgttggtga tgcagtaaaa gctcgtaggg
    16141 cattagatgc aacaaaagaa gcttttgaag tagcattaaa tatataaata ataaataaat
    16201 ttttctttgt aaataactga ataggttatt tgcaaagaaa atgataatat tgggaggaga
    16261 ttatagttat ggaaatttca ggtagaacag gattatttgc attaataggt acaccagttg
    16321 gtcactctaa atcaccagtg atgtataatt atagttttaa gaaattagat ttagattata
    16381 gatatttagc ctttgatatc acagtagata aggtaaaaga ggcactttta gcaataaaaa
    16441 cttttaatat aaaaggagcc aatgttacta tgccatgtaa atctgctgtt acagaatata
    16501 tggatgaact ttctccagca gctcgtataa ttggagcatg taatacaatt gtaaatgaca
    16561 atggaaagtt agttggtcat ataacagatg gagtaggcta tgttcgtaat ttaaaagaaa
    16621 atggggtaga agttaaaggt aaaaagatta caattatggg tgctggtggt gcagcaactg
    16681 ctatacaagt acaatgtgct ttagatggag caagagaaat atctatcttt aatcctaagg
    16741 atgattttta taaaagagca gagcaaacag ttgagaatat aaaaaaagat gtaccagaat
    16801 gtgttgttaa tctatatgat ttagaagata caaacaaact ttatgaggag atacaatcaa
    16861 gtgatatatt gacaaatgca actcttattg gtatgaagcc ttatgacaat gaaacaaata
    16921 taaaagatac tagtgtttta agaaaagatt tagtagtaac agatgtcgtt tataatccta
    16981 agaaaacaaa aatgattgaa gatgctgaag ctaatggatg taaggctatt ggtggattag
    17041 gaatgttatt ataccaagga gcagaagctt ttaatctata tactggatta gaaatgcctg
    17101 tagaagaagt taatgaatta tgctttaaat agataagagt aatatcttaa acaatgaaaa
    17161 aacatacgta tttcaatatt tttaataata aattacaaat tgtcatagtg ggttttgcct
    17221 catagtccag ttaaatttga agctttaact gactatactt taaatccatt atggggatga
    17281 gtttattgta cacaaaaatg ttaataaatt aacaagaatg ggagaaaaat tatgaacaat
    17341 aaaaaatatt atcctactgc catagcgttg tatttttcat attttttact tggtattgga
    17401 atttcaattt taggacaata caaaccagaa ttttcatcaa tgtggggggc taaaacatta
    17461 tcagatggaa cattggatgt aagtattgtt ttagctgtta ttgctgcact aggattaggg
    17521 agacttatat cttatccatt tgcaggacct atttcagata agtatggaag aaaagtaagt
    17581 ggtcttatag gaaactttct acatgcaatt ttctttgttg gaattgtatt ttcaccaaat
    17641 ttttatatag catatgtatt tgcaataatt ggaggggcag caaattcatt cttagatacc
    17701 tgtgtaaccc cttcttgtat ggagatattt gctagtttag gaacaatagc aaatatgttt
    17761 actaaattta caatagcttt agcacaattt ttgcttcctt ttataattgg atttgttgca
    17821 gcaaattcaa tatcatttaa agttattttt atagtaacag cgattcttat tgtagtagat
    17881 gcctttttga tagctgtgtt accatttcca ccagctaata atgttattga taacaaaggc
    17941 aaaacagtta aatcagagaa gatgaaattt acaccaacaa gtatagctct tgtttgtatt
    18001 ggatttacat gtacatctac atttgtatta tggttaaatt gtaatcagga attgggtaaa
    18061 ttgtatggga tggctgaccc tacaaaaatt caatcctttt actcaatggg agtaatatgt
    18121 gcagtattaa taacttcttt acttattaaa aaatatatta aaccaataag aatattagtt
    18181 atatatccta taatagcttt acttatgcta ttagttgtat attttgttca aactccaact
    18241 atttgtatgt tagggggatt tgtaataggt tattcagcag caggaggtgt tcttcagtta
    18301 acaacgtcaa ctgcaaatga aatgtttcca actaataaag gaaaaataac atctatagtt
    18361 atgattgcat ctagtatagc aaactatgtt attttaaata tagcaggtat tattacaaaa
    18421 tcagggggag ttaatggacc aaaatatgtt gtattgttta atgtggcaat aacatttgtt
    18481 ggaattttac ttgcattgtt tgtaaacatg agatatgaaa aagaaaaagt gtatgattat
    18541 gatgtctaat attagtataa tatacgcata gagaagtcta aatctaggat agatttttat
    18601 taaaagtaat taaaattaat aaggatatta tttacagtta taagataggg tatttacata
    18661 attaataata aggatattac tagaatttca atttctagta atattttttt gcgtataaat
    18721 gtaaacttga cgaaataagt agtataatca atataaatta catcatgtaa aggaggataa
    18781 tttggtggag aatgttttta agcgattgca ggaatttaat ggttatgatg gatacaaaga
    18841 atcatttgaa atgaattacc tatgtatata tgaaagtata ccgttaagag aacaagtaga
    18901 gcttgcaaat aatctagtag atgaaatttt aaatatgtat aaaagtgaat ctaatgaaat
    18961 ttatttattg gaggatagta attctaagtc tttaatttgt tatttcgaaa tttttatgaa
    19021 aaagataaat actttggtaa aagaaatgat tattgatgag aaatggttat ataaattaac
    19081 taaagaatta atatataaga gtaaaaaagt tgagtatgta aaactagggt tagttttaag
    19141 tgagaaatat ctaaatgtag aaaacttaag agaagttgta gatacatttt ctaaatctgg
    19201 agaatacgta ttttatctga gtaatactat taaaaaactt gaattttaca atacatattt
    19261 atttaattta tctaaaaagg ctacaggttc aataaaagta tttgctattg ttaatatgga
    19321 aaatttagat tctaaaataa attcctatct aattgaagat ggttataagg atacaaagta
    19381 cgaacgatta ttgatgaact atattatttc aattgtagat ttgaatgaat atcttgaaaa
    19441 acgagattta gataaagaaa agataaataa tttagcacgt cttatatgca attacttgct
    19501 aagtgtagaa tttaaatata taggtaataa attagaatta gtgaatcgat ttttacctac
    19561 tgtagtaaat tatgggacta actttgaaag tttatattca attttcctaa tagctataaa
    19621 cgtcttaaaa gatgaaaata tagagtgcaa taagattgaa tttgaaaaag aaataaatga
    19681 tatcttgctc agtgaaaaat ggaaaaatat atattttgaa gcattaagag atgctagtgg
    19741 aaaaacagaa gatattataa aaatgtcaga aatttatgat gtaaacttat cttttgatga
    19801 cttgttgcca tatctaaata gagatattag agattttgag gtatattggc atatatctaa
    19861 aaagggaacg acaagttcta gattaaaatt attaaatttt tttgaagaga cgtttaaaat
    19921 agatgattta attggaaaaa tgaaagatat agaaaaagat aaattgactc aagaatatta
    19981 tgatgatatg ttatttttta ttgtgcttaa aggaagtaaa tcactatatc ctgaaggaaa
    20041 gaatatatct ttaaaaggaa tatttggaaa tataaatgaa gtaagaaaag aatccataaa
    20101 tatcttaaaa agatacagag agaaactatc tcttgaagaa ttaaaaattg ttaaagaagc
    20161 gtatgaaaaa gaaaaaaatg ttatattgaa ggatgaatta agaagagtat tatatgaaag
    20221 taataattta aaaaaagaat ttgtaaatat tgaaaaaata aaagtagatg aacatggtaa
    20281 ggatatctat ttgacttcaa tagctgttgc tggttcaagg tttagaaata gagaatatct
    20341 tgaaaaagaa ttggagaaaa gtaagatata ctatttaact agagagaaag ataatctata
    20401 tgatgaaaaa gctattaaaa tagttggtga aactggatat gtaataggct atgtcccaag
    20461 aaaagaaaac tatattttga gcaatttatt agatggagga aaactgctgt attgtagagt
    20521 aactgaatat aatttatatg aagattgtat ctacgcaaat gtgtacttga gttataaaga
    20581 tgtaattgaa acagttgaaa actctttaaa gatggtttta gataagagta gaataaaact
    20641 tataaactaa gacgtttata tattgcttag atgatgtaat cttaactgat tggttatttt
    20701 ttctggaatt atggtgtatt ttatgagcaa tgctaatgaa tataaaataa aaaaacaatt
    20761 gttttgtata tatgatgtat ataaatatat ttatatattc atatttacaa aacaattgaa
    20821 atttatgtta ttttataatt ttaaatttag atggaatttt aacttctgct ttatctttta
    20881 ttttagaagc ttgtttttta actttattta ataattcaga ttcatctaca actaatactt
    20941 ttctttcctt catcaaccat ttaccatcac aaatagtaga gtctacattt tcactagtca
    21001 tagaataaac tatatttcca attggattat gcattggtaa acaatgaatt gtatctggat
    21061 ttagaataat catatcagct ttttttccta cttcaagact accaatttct ttttctagta
    21121 aagcacattt tgctccattt atagtagcca tttctaagac ttcttcagct ggtacgacag
    21181 ttgggtttaa agttcttcct ttatgtatta gtgatgtaag atacatgtcc ctcataaggt
    21241 ccattctatt atttgaagga gctccatcag taccaattga aacaggtatt cctttttcta
    21301 acatttcagg aattgatgca aatcctagaa ctactttcat ggctgcagct ggattatgag
    21361 aaacttttac atcatgtaat ctaaataaat caatttcttt atgagtaagc caaactgtgt
    21421 gagcagctaa aagatttggt cctaatacac ctagtttatc aagatattca actgttccaa
    21481 ctccattgtt ttgctttaca aatgaaattt catcagctat ttcagcaata tgcatatgta
    21541 tacctgtatt gtatttatca gctagttcct tagttttaac aagtaattct tcagttgtat
    21601 taaaaatagt tcttaatgca aaccatattt taatcctctc attagatgtg ttattaaact
    21661 tatggaataa gtctaattga gtgttgattt cctcatcagt agttttttgc catgcttcag
    21721 gaagaccatc ccctgtatcc ataactgatt tagctaaaca ggctctaagt ccagttttat
    21781 caacagcttc aaccatagca tctacatatt gaccaccaga ttctaaaaat gtagtgaccc
    21841 cagattttat caattcaaca caacatgcag tagatgaaat caatgaatcg tcatagttga
    21901 agctactttc gtatggccag attctctctc taagccaagt taaaagtaca acatcatctg
    21961 caagaccacg tcctaattgt tgggataagt gaacgtgtgt attgactaac cctggcatta
    22021 gtatttttcc tttagcatca tatatctctg catctttatc tacaatatta atatcaaact
    22081 taccaacatt ttttataata ttattttcaa taagtatact tccagtttca tatacctctc
    22141 tatctgcatt catagtaatt agatatgcat tttttataag taattgtgac ataagtacga
    22201 cctcttttct atatattttt atatctatgt attatgataa taaattttct atacaaagtt
    22261 aatagcaaaa tcaatttata cataaattga ttttgctatt tataaattta aaataaataa
    22321 gattttattc tatataattt aaaatattga cctagaacta tttaaaaata ttaacttaga
    22381 actaagttta caactgcaag taaacctata atccaaagtg taatactgat tttatcagtt
    22441 tctttagcta ctacatgcat tattatatat gaaataaatc caaagcataa accaatactt
    22501 atactataag ttaaaggcat caataaaact gtaacaaaag caggtacagc tattttgcta
    22561 tcagcaaaat caatctgttt tatgctttca aacatgaata caccaaccat tacaagtgca
    22621 ggagctgcag cgtaagtagg aactatacca agtaatggtg taaatataag tgtagctaag
    22681 aataatgctg ctgttactat tgaagcaagt cctgttcttg cacctgctgc aataccagaa
    22741 gcagattctc caaatgttgt aacagtacta gttcctaaga atgagcctat taaagtagaa
    22801 gaaacatctg catataacat tcttcctaaa tcttttattt caccattctc atcttcaaga
    22861 cccatttgtt tacaacaagt aataagaaat gctaatgaat caaacatgtc tataaacatg
    22921 aaagagaata taggtccaat taatgagaat ttcaatgcac ctacaatatc taatttaaaa
    22981 gcaactggtg ctatactagg aggtgttgag attattgctg ttggcaactc cacaaatcca
    23041 aatataacag atattattgt tgcaactact atacttatca atattccacc tttaactttt
    23101 ttatattcta gtattgccat taagataaga gcaaaaaggg ctattaaaac tgttggtgta
    23161 aaatctgcaa gtgaaactat agtatctgga ttagctatta taacacccat attttttaaa
    23221 ccaacaaaag caaggaaaag acctatacct gctgaagatg ctactgccaa ttctctagga
    23281 attgcgtctg caattgtctt tcgaagacct gttactgcga gtgtaaagaa gacacatcca
    23341 gatagaaaaa ctaccccaag acctgtttgc catgggattc ctgcacctat aaccaaagag
    23401 tatgtgaaaa atgcatttaa tcccatacca ggagctagag caaatggtgc atttcctaaa
    23461 atcgcaaata taagtgtacc aatggctgta gctagtatgg taactgttat aagggcggct
    23521 ttatccattc cagcttcaga taatattgct ggatttacga aaactatgta tgccattgtt
    23581 aagaatgtag taactccacc tattacttct gttttaaggc tagagccacg ttcgctaaat
    23641 ttaaacattt tgtctaaaaa gcttaatgat gaattatttc cacctggttg tacattgtta
    23701 gtgtttggta ccatttttac ctccaaaaaa ctttaagata taaacaaaaa aatatatatt
    23761 catatagata gaatagaaca tatattcaga gttgacattc tattcataga aaacccattt
    23821 aaggtgagtt tgtagaaact attagaccgt attactaata ttatatgaac attttctatt
    23881 tgtttttctg ttgtattata tcgtaatatt gtattattta caataaatat aataaaatta
    23941 ttcgtttttt tgtcgatttt ataaaaaatt aaggttattt attgaaaaaa attacttttt
    24001 ccaataattg aatatgtatg agtataatgt caattattct aacttgtttt atactattaa
    24061 tggaagttta ttataaatga gtttgtgata taattataaa agtactgaat acatggatat
    24121 ttcgtaaaaa ctttttaata tatcaaatat gtatattaag ttaatatatt aaaaaaaata
    24181 atatcaaaaa taatataatt tatagtggag gggaaaatga ctaaaattat taaagtgcaa
    24241 aaacctaaat ttacaggaga attagaaatt gttgaaaatc ttgaagatat acttgaagat
    24301 atatttaatg atgaaaaaat atttaataaa attatagaaa atgaagttat aagtgattta
    24361 gattctgtaa gagttagctt tgaatcttgt atatttagaa atgtgtcttt tgaaaattgt
    24421 aatttaaaaa agattgattt acttgatgtg gtatttgaaa actgcaattt atcaaataca
    24481 ttttttgatg gaggaagtat atacagagtt gaatttaaaa atagtaaatt gataggagtt
    24541 agatttgatg attgtatact taaaaacgtt ttgttcaagg atgttttagg taagtattct
    24601 aacttttcat tctctaaatt taagatagtt tctatcttag attcaaattt tgaaaattct
    24661 gtatttcaag aagtaaaagg tgacaatcta gtatttgagt atacaaattt aaaaaaagca
    24721 aactttaatg gaactaaact ttctggcata gattttacta ctaataatat tgagggaatt
    24781 gagattggta tagatgatat tagaggagca atttttgatg tgtcacaagc aattgattta
    24841 acgaaattga tgggaattat cataaaatga aaaaaacatt cataaaataa attgggaaaa
    24901 cgttaacaat atgtgaattt ttaccagatt gacatttttc tgaaaataaa gtaagattat
    24961 cttaatattc aaaatatttt caaagggggt aatttagtgt gagtaagcag atgagagata
    25021 tttttatagt tggttttgct ttattttcta tgttctttgg tgcaggaaat cttatgtttc
    25081 ctccattttt agggatggaa tccggcaaag actggttaat tcctcttatt ggatttgttg
    25141 ttgcagatgc tggtatagca atgcttgtta ttatagcaac agcaagatgt aagggaagca
    25201 tggatgatgt acttaggaga gctggtaaag ggttagcaag agtaatgagt gtagctgctc
    25261 tagcatgttt agcacttctt gtaataccaa gaacttgcgc aacaacatat gaaatgggta
    25321 ttatgcctat atttggagac catggtccag tagctaaagc tgtattttca ataatcttct
    25381 ttgggctaac tttagcattt actattaggt cttctaaggt tatagacata ataggtaagt
    25441 atttaactcc agcgttatta ttagtattat ttgttttaat agtaaagggt ataatttctc
    25501 cagcaggacc tatgagtcct gagcatatga tagataaaaa tctatttggt gaaggtatat
    25561 cacaaggtta tcaaacaatg gacgctctag gtgcagcatc tatggcaact ataatgttga
    25621 tgtctataat agctaaagga tatacatcag ataaagacca aataggaatg actattaaag
    25681 ctggttttgt agcctgtgta tttctagcag tagtatatgg tggtcttact tatttaggtg
    25741 caacagtttc aactttatat gatactagta tacctcaagc atctttatta gttgaaataa
    25801 ctagtatatt gttatatggt caagtaggaa aggtaatttt aggtgtaatt gtggcacttg
    25861 cctgtctgac tacttctaca ggtttaacag catcaatatc aacttatttt gaaggaataa
    25921 gtaaggcgag ttataaacaa ctggttatag gtatatgtgt agctagtatg cttatatcaa
    25981 atttaggtgt tgattctata atagccatat cagttccaat cctacaaact atatatcctg
    26041 ttttattagc aatagttgtt atggagttgg ctggtaaaca tataaagaat gataatgctt
    26101 ttaaaggtgc agcatatgtt acattagtga ttagtttatt atcagctata aatggaatga
    26161 caggagctgt tccatttata caaagcttgc cacttgcagg tttgggcttt aactgggtaa
    26221 taccagctat attgggtgga atagttggta actttataaa aagcaacaaa caagtagcat
    26281 aataatttgt aattaattta attttataat atattaattt ttagtactat gggtaggttt
    26341 aatctaaacc taatctaccc atagtacttt ttagattgga ttataatagc atgtatataa
    26401 ttttttataa tataattttt ctttgaagta tgataaaatt agtaatagtg ataagaatgt
    26461 acaaatttag gaggttttaa ttaatgctta gaaaaataac agcatttatg ctatccatat
    26521 gtttaattat tggacttaca ggttgtagtt tacttgtagg caagaaagat aaagagaaaa
    26581 ttatagatga ttttaaagtt gcagtggtaa ctcaaccatt atctgaaaat aaagtacaat
    26641 acaatatggt ggaagagatg gcaaaagaat atgaggaaga gaataaaatt gataaagaca
    26701 aagatggtca aactaaagtt aaacaaacta taaagcatgt tgtattgcca gagaatttta
    26761 cttctaatat agatagtgct attaataaga tagtaaagtt ggctgatgat aaagaagttc
    26821 aggctattgt agtttcaact gaccaagcag ggcttttacc tgctttacaa aaggtaaagg
    26881 aaaagcgtcc agaaataata actatttctg cccctatggg agatgataaa aaccaattaa
    26941 gccaatttgt tgatgtgaat ctaggggtta gtgcagagga aagaggaaaa gtattagctg
    27001 aacgttcaaa agaaatggga gcaaaagctt ttattcatta tgcatctaca gatgatttga
    27061 aagatgttaa tatagccaaa agattagaaa tgattaaaga gacttgcaaa aatataggat
    27121 tgccatttgt acaagttaat actccaaata taaatactga agaagacaaa agcaaagtta
    27181 agcagttttt aaatgaagat attgaaaaac aagttaaaaa atatggaaaa gatataaatg
    27241 tatttggtgt aaatgagtat atggatgaag ttatattgac taaggcttta gaattaaagt
    27301 atatagttgc agagcaaagc aatccatcac caatacaaac atatcctagt gttatgggtc
    27361 taaaaatatc tgaaaaagat gctcaaaatt atgataaaat aaacgacatg ataagtgaaa
    27421 aggctaaggc ttttggcatg tcaaatagac ttggtggata tcctatgcca atggatgctt
    27481 ttctaccatc agtagcaatt tatcttgcta ctgaaatggt gaaacaagat ttaacacaag
    27541 aagatgtatg tgacccagat tatttagagg cgtttacaga attaagattt ggaatcggtt
    27601 cagaatttac tcctttaaca gaggtacttt ataattatca atcagtaatt ttaagccaat
    27661 taatttatta atattaagga agtgtctcaa aatgaacttt ttagtttatg aggcactctt
    27721 tttctattaa atc
//
LOCUS       DBGJFF010000044        27454 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.044, whole genome shotgun sequence.
ACCESSION   DBGJFF010000044 DBGJFF010000000
VERSION     DBGJFF010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27454
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.044"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(155..2173)
                     /locus_tag="ACOIAF_001882"
     CDS             complement(155..2173)
                     /locus_tag="ACOIAF_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HGZ2668893.1"
                     /translation="MTKKLVISKTKLFIFIIAFISIFIYLFGSKNTLIGVGIVTAMLT
                     LLERDLTISPIKNLLKYLAINIILGILSFFAVQNMYLGVLLNFIALFIIGYVFSYDLK
                     RAVYVPFGLMYIFMVSIPVGKSEFPMRLSALAVGAVIIMIAQFVMNRNRMKNVGDKEL
                     ISICDELLEKISLLKNTINDDSSIIKSSMRKIDSCNYRINSISKNLKMVIFDNRKDDF
                     YISIRGIDIMNILFSLERISLILERYKKDSKEFEDEDIKNILEESSINSKSDVLVVAT
                     KEINYIKMCLENKDTITDKEILGFRDYVIAQDTKNINLKEIYSVLENLYEFLVDYKKV
                     KSRDEKKAERKSKIPHEFKRLSIYKKNFNLNSIRFSYAVKIALATAVAGFIMDYFHLR
                     DGRWIMLTVFSLTQPYAENCIQRSRKRIEGTFIGAVIFIVLFSIIKDSTLRSLIVLLA
                     GYINSYVVDYRKLMVCVTVSALGSAVVMGDPNVLTISRISYVALGAIIALIVNKFILP
                     YDAKTGYQHVIEMYKGIVKNIIDEVNKSIENVADVYYIKNLLLIPSLIEDRLMLINAI
                     YKDKHQEDFLENQKLLISNMYNLYINVNKNKIKDEDVEKILRDTNYISNYNVDKYDEG
                     RSVILESIVNTKSLGDKIICLNLLQTLNGVKEMYRISNITKVSIKEAA"
     gene            complement(2444..3220)
                     /locus_tag="ACOIAF_001883"
     CDS             complement(2444..3220)
                     /locus_tag="ACOIAF_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HGZ2668894.1"
                     /translation="MHKETLDKLTNAAINKINLLNTSKVKYLVSSAFAGLYVGIGILL
                     IFTIGGLLTDAGSPMTKIVMGLSFAIALSLVIMTGTELFTGNNMVMSAGMLNKGVSIK
                     DTSKIWAYSWVGNLIGALVLGIIFVGTGLVDKGPVAEFFANTAASKASMPFTALFFRG
                     ILCNILVCVSVLCSFRTNSDTAKIIMIFLCLFAFITSGFEHSVANMTIYSVSLFSPTI
                     STVTIGGAIYNLVAVTLGNIVGGALFMGLGTYILGKEKLN"
     gene            complement(3277..4248)
                     /gene="asrC"
                     /locus_tag="ACOIAF_001884"
     CDS             complement(3277..4248)
                     /gene="asrC"
                     /locus_tag="ACOIAF_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016002 - sulfite reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit C"
                     /protein_id="HGZ2668895.1"
                     /translation="MIRDLNTKKVMKNAYRITKTKYETSLRVRVPGGLIDPESLAVVS
                     KIASEYGNGQVHITTRQGFEILGINMEDMEEINKIIQPVIEKMNINQSEKNAGYPAAG
                     TRNIAACIGNKVCPKAQYNTTEFAKRIEKAIFPNDLHFKVALTGCPNDCIKARMNDFG
                     IIGMALPIYEKDRCVNCGACVKKCSKISVGALKTENNKVVRDKDKCIGCGECVLNCPT
                     NAWTRDEKKYYRLAIMGRTGKKNPRLAEDFLLWVDEDSIIKIILNTYRYVEKYIDKDA
                     PGGKEHIGYIIDRTGFMEFKKWALEGVELPEITKMYENIYWSGIKYL"
     gene            complement(4261..5052)
                     /gene="asrB"
                     /locus_tag="ACOIAF_001885"
     CDS             complement(4261..5052)
                     /gene="asrB"
                     /locus_tag="ACOIAF_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016002 - sulfite reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrB"
                     /protein_id="HGZ2668896.1"
                     /translation="MNPYIPVSAKILDIVKHTDKEWTFRVNCNTKGVLPGKFYEISIP
                     KYGESPISVSGYGEDYIDFTIRNVGKVTSELFNYKEGDSFFIRGPYGNGFDVSLYEGR
                     EIVVVAGGSALAPVRGIVEYFYNNKEKCEKFKLIVGFKSPKDILFADDLKRWSKKLDI
                     LVTVDGAEEGYSGNVGLVTKYIPELDMKDINNTSIVVVGPPMMMKFTVEEFLKRDLDE
                     KNIWVSYERKMCCGIGKCGHCKMDDTYICLDGPVFDYSYAKNLID"
     gene            complement(5053..6060)
                     /gene="asrA"
                     /locus_tag="ACOIAF_001886"
     CDS             complement(5053..6060)
                     /gene="asrA"
                     /locus_tag="ACOIAF_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016002 - sulfite reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrA"
                     /protein_id="HGZ2668897.1"
                     /translation="MKLKLDSSKFNEGLNYLKKDYKIFAPVLIPFKGTFSDTDVIRYK
                     EVNAFEEMEFAKKANFSPKEVVLPINQVLFYFTEKEFKESDLDNKKILIFLRACDING
                     IKRLDEIYLNNGEEKDYFYKNIRDKVKFALVGCKESFRNCFCVSMDSNKTDNYSIGLN
                     IEGDTLYLDIKDSEFEVFNGEATEFNVDYVTDNLISVDVPENIDSNEMIGNSIWDEYD
                     TRCIGCGRCNFVCPTCSCFTMQDIFYKENENVGERRRVWASCQVDGYTDIAGGNSFRK
                     KQGERMRFKTMHKIHDFKKRFGYHMCVGCGRCDDACPQYISFSKCIEKINDLVISKEE
                     V"
     gene            complement(6451..7140)
                     /locus_tag="ACOIAF_001887"
     CDS             complement(6451..7140)
                     /locus_tag="ACOIAF_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HGZ2668898.1"
                     /translation="MSKLKDYNIDKVIIFNNLSTKTKNQLKEKVKLKRLQKKEILFYE
                     KDSLDSVYILLEGKVSIFKISENGERKVIFILNSGEVINDITLDASKNSTVGCEAFEN
                     SIVAYYSIEEFLEIMQNDFQLTKDIISYMERRIRRLYRQLKNSISIKVDKKLAAKLYR
                     LSREFGVCCGEWTLLNVNITVTYLADMLGCKRETVSRMIKVLQKEELIKIDNKGFYVK
                     ETELSRYFKNN"
     gene            complement(7203..8051)
                     /locus_tag="ACOIAF_001888"
     CDS             complement(7203..8051)
                     /locus_tag="ACOIAF_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HGZ2668899.1"
                     /translation="MSILEQLESPTFKVTKSDKLLIAYIKENIDDVFYKPISQIAKES
                     NIGEATITRFVKKMNFNGLQDFKVTLAQEISTINKKNIINKNIQNDEPALDTAKKLLS
                     SNVTTLENTVEIINSKDVHDCARLIINAKKVYFIGIGYSGIIAQDSNYKFMRIGLNCV
                     SFDSSHTMIMMSSIMEEGDLIIAISHSGETEEIIKTVKLARANNAKIISITENKNSEL
                     KDISDVHLSYVSGETVLETGSISSKLAQFFIIDLVYTQVVKELSNEAIERKIKTTNAI
                     KLFKNE"
     gene            complement(8070..8957)
                     /locus_tag="ACOIAF_001889"
     CDS             complement(8070..8957)
                     /locus_tag="ACOIAF_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HGZ2668900.1"
                     /translation="MKNYVCIDVGGTSIKYGFIREDGLIIDKSSLDTEAKEKGGEGIL
                     AKIKDIVRKYIEKNEISGICISTAGMVDPVEGKILFALEELIPNYKGMQLKKEVEKEF
                     NIKCEVENDVNCAGLGEMWLGAGKDATSSICLTIGTGIGGCIIINNKLINGFSNSAGE
                     VGYMNVNGSSFQELASTSSLIKKVAKIKNLNENDLNGKIIFDLAKNNDEDCLKELDNM
                     IKSLAVGIANICYVINPEVVILGGGIMAQEKFLKPKIDKALKEVLIERVYKNTNIEFA
                     KRQNDAGMLGALYNFLNKI"
     gene            complement(9015..9818)
                     /locus_tag="ACOIAF_001890"
     CDS             complement(9015..9818)
                     /locus_tag="ACOIAF_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HGZ2668901.1"
                     /translation="MENIDVFLLIGQSNARGLGNPKESIIPNENCFEYLSTDEIINMR
                     CELETSEGDGTIAPAFSNEWNKLTGNKVCFIHNAKDGSRIKNWNHDNNWFLNDTIEKF
                     NAGCTTLSKHYNIENKYVIWIQGESDAKYGSDALYYKESLKKIAYRLKEECMIDKMFV
                     SLTGYWLGEDEYFIRTRRIAAAQESACNECDILCVGSKIAMQFHDRGLTIDDVHYTQE
                     GLNMLGEDLCKNIYKYHITKEKSVLSDTIDLSEARKYICELEKISKRFV"
     gene            complement(9978..10430)
                     /locus_tag="ACOIAF_001891"
     CDS             complement(9978..10430)
                     /locus_tag="ACOIAF_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HGZ2668902.1"
                     /translation="MIFGNINHSKTYSTLHEDLLRCFEYAKDNSLIDYEKGTYTIDGD
                     DVFVNIVEYRTCEKEDRFWEAHKKYIDVHLMLDGCERIDINFIENLEQKIYEEEGDFL
                     PLDGKNNGYVELRKGDFLVCYPEDAHMTGIKVLEKENIKKAIFKVLVR"
     gene            complement(10560..12062)
                     /locus_tag="ACOIAF_001892"
     CDS             complement(10560..12062)
                     /locus_tag="ACOIAF_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HGZ2668903.1"
                     /translation="MVGFTWIDFAILVVYLLAVLFAGLLFSKKEMKGKEFFKGDGTIP
                     WWVTSVSIFATLLSPISFLSLAGNSYGGTWILWFAQLGMFIAIPLTIKFFLPLYSRLE
                     IDTAYEYLEMRYESKGLRVLGALMFIIYQIGRMSIIMYLPSIVLAGLTGIPVNVLIIV
                     MGVIAIIYSYTGGLKAVLWTDFIQGMVLIVGVTGTLFYLIASINGGFGTVMDTLTTGH
                     KFLASNEVMFDKNILSTSAFIIFVGAGLNTFSSYVSSQDIVQRFTTTTDIKQLNKMTY
                     GNGVLSMGLATVFYLIGTCLFIYYTQNPDLAQTVQQDQVFASYIAYELPVGITGILLA
                     AIYAASQSTLSTGLNSVATSWTLDIQTIITKDISMEKQTKIAQYISLGVGILSIAVAI
                     VLANGEIKSAYEWFNSFMGLVLGVLAGIFVLGAFCKKATKMGAYVGFIVSAILVVYLK
                     YRMPEVTSWAYSLITITTSVVVGTIVSAIEAKVKAKVSLPKAETTVYYEK"
     gene            complement(12095..12976)
                     /locus_tag="ACOIAF_001893"
     CDS             complement(12095..12976)
                     /locus_tag="ACOIAF_001893"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HGZ2668904.1"
                     /translation="MRTTDMKGIYSALLVSFDKEGNINEKGLRQIIRHNIDVCKVDGL
                     YVGGSTGENFMLSTDEKKRIFEIAKDEVKEEIKLIAQVGSVNLKEAVELAKFTTDLGY
                     DAISAVTPFYYKFDFEEIKHYYNTIINSVDNRLIIYSIPFLTGVDMSLDQFGELFENE
                     KIIGVKFTAADFYLLERMRKTFPNKLIFAGFDEMMLPATVLGVDGAIGSTFNVNGVRA
                     RQIFELTKNEKISEALEVQHVTNDLITDILGNGLYQTIKLLLEEQGVEAGYCRQPMKE
                     ATDEMKSRAKEIYRKYF"
     gene            complement(13046..13717)
                     /locus_tag="ACOIAF_001894"
     CDS             complement(13046..13717)
                     /locus_tag="ACOIAF_001894"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HGZ2668905.1"
                     /translation="MLDKVKGRLIVSCQALEDEPLHSPFIMGRMAKAAMEGGAVGIRA
                     QGVEDIIEIKKVTGLPVIGIIKRNYEDSDIYITPTKKEVDELLTTGCEMIALDATNRV
                     RPNNEDLKELIKYIKENGVLVMADISNYDEAIKAQEYGVDCVSTTLSGYTPYTKTLEG
                     PDFVLMERLVKDLEIPVIAEGKVNTPQDLKKVFELGVHSSVVGSAITRPQLITEKFVK
                     AIEIN"
     gene            complement(13990..14481)
                     /locus_tag="ACOIAF_001895"
     CDS             complement(13990..14481)
                     /locus_tag="ACOIAF_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HGZ2668906.1"
                     /translation="MEGNTNNKVDDKFSKVKKKFGSGKFKVELYKEKSKLNREIDDIQ
                     EKKAKIFLEMGILTYQKIREDSINDNSFDKFCDELLELDKLIYEKNMEINEMEMAESN
                     VTCECGYVGNVNDKYCAECGKKFELENECDDFIICGYCESEIDTEAEFCPCCGRKIIM
                     DLE"
     gene            complement(14503..15867)
                     /locus_tag="ACOIAF_001896"
     CDS             complement(14503..15867)
                     /locus_tag="ACOIAF_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HGZ2668907.1"
                     /translation="MGEKKINNMNYSHNNYSKKTGEPTRKIKNSHITLKSKEEKICSS
                     CNKENNTKDNYCKFCGNELYEIASLRPLETKLDLKSKIKELSYHANKRGVFLTTFTTI
                     FILFIIALIFKAIITIQFNDISYLVNPAHIILALNLGQISVSMSTMMGSGFINANIGL
                     LILLIIPILVLLISNLIFMRKRCRDSKTVLANSLGVGIFYGLVLAILSIFTNVKTSSH
                     SMLEYGYALEYSYEFFSVLLNGFVLGFICTYITTYKKSYEKENMYLSLFSNSIRTFIL
                     GYVLVLVILLVLTISDSSYLNELDMSSYSNGLNLFTILPQIASYMWAFANGISVTIIN
                     STVSMFTLSSSSLFGDTKLMFYAMGALSMLILLLNGYKLRFKYNTDSIRPIIVFSIYY
                     AFLMGILALFSTFILDSNINFFNTTNYGTTLIMQFKVLQAIVISFVYSFVISLIGYKL
                     NSAD"
     gene            16286..17461
                     /locus_tag="ACOIAF_001897"
     CDS             16286..17461
                     /locus_tag="ACOIAF_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HGZ2668908.1"
                     /translation="MNLEKIKKFFGFPLDKKHKDNVEKELCNYLYHTGKIICIIIVIS
                     QIIMMISISAREGGPFVTPRRQWYFYMYVTLFCVSSVFMFVFSYLWNKKKDCCTFYIN
                     LWIIYVTFFCLWGCVITLLDQLGGNDLSVFSYIMLAAAAFSVLKFGESVIVFGGCFLF
                     LNLFLPGIQTIPGNEFSNIVNTFFITSLAIFVSTMLYRNRVFTYYDKLLISQQYEEIS
                     HINNLLNDKVMTDDLTKMNNRRYLEDILQKKLISHANQEPITGMMIDIDYFKQFNDNY
                     GHQKGDYCLQNIAKVILDFTSSENEHAIRYGGEEFFICIFNCNEADAKLKSECLRKII
                     ESCNFERDDVPLKCVTVSIGVYTKKIGKTITLNEFISNCDKALYTAKEQGRNCVAFY"
     gene            complement(17634..19031)
                     /gene="asnS"
                     /locus_tag="ACOIAF_001898"
     CDS             complement(17634..19031)
                     /gene="asnS"
                     /locus_tag="ACOIAF_001898"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HGZ2668909.1"
                     /translation="MQKEFITVKELYREKEQYIGKTVKVAGWIRTSRVSKNFGFIELN
                     DGSFFKNMQIVFDEKVENFKEVGKLAISSSILVEGELVSTEGAKQPVEIHAKSIVVEG
                     ESDSSYPLQKKRHTLEYLRTIAHLRPRSNTFSAVFRVRSIAAYAIHKFFQERNFVYAH
                     SPIITGSDCEGAGEMFRITTMDLNDIPKTEDGKIDYTQDFFGKEANLTVSGQLNAEIM
                     ALSFRNVYTFGPTFRAENSYTGRHASEFWMIEPEIVFADLEDNMELAEDMIKYVISYV
                     LENAPEEMEFFNSFIDKGLLERLNKIVNSEFIRITYTKAVELLIESGHEFEYPVEWGC
                     DLQTEHERYITEQIYNCPVFVTDYPKDIKAFYMRMNEDGKTVRAMDLLVPGVGEIVGG
                     SQREEREDVLLDRINEMGLREEDYWWYLELRKFGTATHSGFGLGFERIIMYMTGMSNI
                     RDVIPFPRTPKNAEF"
     misc_binding    complement(19108..19341)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(19510..19704)
                     /locus_tag="ACOIAF_001899"
     CDS             complement(19510..19704)
                     /locus_tag="ACOIAF_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3787 domain-containing protein"
                     /protein_id="HGZ2668910.1"
                     /translation="MESKKARALKGKDNKRLKSSLLVGNESTAAWADVEKLKPHSKVS
                     IPSIKGVEEAKDWVDNGSKL"
     gene            complement(19977..21650)
                     /gene="cspC"
                     /locus_tag="ACOIAF_001900"
     CDS             complement(19977..21650)
                     /gene="cspC"
                     /locus_tag="ACOIAF_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid germinant receptor pseudoprotease
                     CspC"
                     /protein_id="HGZ2668911.1"
                     /translation="MEKSYCIIYQGDIESALQENGINRYMVLNSQLAVIYVPLDFDET
                     ILNNIIQVAWWEESEPMSSLIEITNNVNNGETITTAAETDYIYENPYNDITGRGILLA
                     VIDSGIDYLHPDFINDDGTSKVLYLWDQEANTNPPPEGFIFGSEFTRSELNIAINRND
                     GSLSQDNIGTGTLVSGILVGNGRINSQYRGITTESDLIVVKLKSYTDTYYAGRINYSV
                     SDFLAAITYVTNIARTENKPLIINLTIGVKSSAVATTSILDTFNILSSAGVVVVSGAG
                     NQGNTDIHYSGRFSSVGEVQDVIIQDGDDYALDITLNTNGPDKVGAQIISPSGEVSHD
                     IRYSPDFYIYRGKFNLENTTYAMRFIYPYITSGKENLEIRLRDIKPGVWILRLTSELI
                     INGEYDIYLPNKNLIAPDTRFLDPDSVATITMYAASDDVITVGTFNNKTDSMWIGSSK
                     GPIRGRGIKPDIVASGVDIISTYKNGTYNTGTGTGVSSSIVTGVLALLMEYLEKQDNV
                     PRLSLFTQVLKTYLILGATKLEIYTYPNVSQGYGILNLKNTIQQIANTL"
     gene            complement(21660..25058)
                     /gene="cspBA"
                     /locus_tag="ACOIAF_001901"
     CDS             complement(21660..25058)
                     /gene="cspBA"
                     /locus_tag="ACOIAF_001901"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional germination protease/germinant
                     receptor pseudoprotease CspBA"
                     /protein_id="HGZ2668912.1"
                     /translation="MIIINYELIVKYNGDILRLEEELGVSVEILNSSYAIITSSNEED
                     VNILLTYPEIEFIEKPFILQTQDVQSFSSTGITGFKNRTGLTGKGTIIGIIDSGIDYT
                     LPVFRDSDGRSKILYYWDQSIQGNPPEGFREGTLYTNEDINNAIDGSMYIPISTTSLH
                     GTHVAGICATIASDARIIVVRVGNIQTDIFSRSTEFMRAIKFILDRALELRMPVTLNI
                     SYGSNEGSHRGTSLFEQYIDDMCLFWKNNIVVAAGNNADKGGHKRIRLQNNITEEVEF
                     IVGEGERILNINIWPDFVDDFSVHLVNPSNNQTQAISLTSGEIRNTLGETRITGYFYP
                     IAPYSLTRRVTLQLSSNTQITPGLWKIVFEPIDIVTGNVNIYLPTSEGLNRNTRFLIP
                     TQELTVTVPGTASRVITVGSFNSRTDIVSIFSGEGDTQLGVFKPDLLAPGEDIVSFLP
                     GGTSGALTGTSMATPHVTGVCSLFMEWGIVNGNDLFLYSQKLRALLLKGARRLSNQSY
                     PNNSSGFGFLNLSDIDLYTLSNINQDLETEDMGYRSINKSFKDEENSYKFIDGYNMQI
                     HNDLENEIYISKNASRQSGILSGIDIVHTPEFEEELAGLGMSQRFFKISDSLGVLSIN
                     NTDYSSIQRVLQLPSIIRTVSTTKMTLLGEINRGTFGGVVATEEMGVNFFKNNPNINI
                     TGRGTLISIADTGIDYLHPDFIYPDGTSKIVYLWDQTKEGTPPDGFYIGTEYTREDIN
                     RAIAENDPSLSQDEVGQGTMLSGICAGLGNVNSEYAGIAEDSELIIIKLGKIDDFYNS
                     AMLFAASQYAYKKAFELGRPLVINMSLGTSSLAGLTNRSNSEKAFFTRGLCITAGAGN
                     EGNTQTHTSGIIPYVGGSVEVELELNEDEEELSLELWLNRPDKADVIIVSPTGEESKS
                     VGISNYNKVTGLFDLEGTEYSITYIYPTTFSGQQFTNVTLKNAKRGVWKIRLVGVYII
                     TGRYNLYLPNRELLKSGTRFREVDPFYTINYPAIQDDLITIGAYNTINGSLWQSSSRG
                     PTIEDRLKPDIVAPGVNIIAAYPGNTYATITGTAAASAHAAGAAAMYFQYTFVDGRYP
                     NQAYVQKIKTFMQAGARKDSNTVYPNTNSGYGLLDVRGMFDVLR"
     gene            complement(25225..26421)
                     /locus_tag="ACOIAF_001902"
     CDS             complement(25225..26421)
                     /locus_tag="ACOIAF_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HGZ2668913.1"
                     /translation="MFIEFFYLLRSRGLNVSLNEWMTLIEALDKGLCYSNFSNFYYLC
                     RMILIKSESDFDKFDAVFLEYFKGIEHQEEIPEEIMNWLKKPDIDLEEFERLEQNPNI
                     NLDKLRAKLEERIKEQDSPHNGGNYWIGTGGTSELGHSGKGQTGIRIGGNSTYGRAVL
                     EVAGERKYRDFRDDEVLNMRQFQVALKRLRQFSTRIDAPKTELDLDKTIEETCNNAGY
                     LKLFFEKPRKNTVKLLLLMDSGGSMRGYSTLCNTLFQSVSKSNHFKDVKIYYFHNCIY
                     DRLFTTPECWLSKSINTEWILKNIDKNYKVIIVGDASMSPSELLHIGGNYRGPYNYTP
                     GIEWLKRFKRKYSKIVWMNPELRDGWDSINYWYQTQRMIQSEFNMFPLTVKGLEKALK
                     NLMVSR"
     gene            complement(26442..27263)
                     /locus_tag="ACOIAF_001903"
     CDS             complement(26442..27263)
                     /locus_tag="ACOIAF_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HGZ2668914.1"
                     /translation="MIKKEISNFRGSSDYVVSPELMASVNVAIALEKPLLIKGEPGTG
                     KTMLAQAISNELKKDLVIWNIKSTTKAQEGLYVYDTVQRLYDSQFGGEGVDDISKYIK
                     YGKLGEAFSSNQQVILLIDEIDKADLEFPNDLLWELDKMEFYINETKETVRAKQRPIV
                     IITSNAEKELPDAFLRRCIFHYIEFPDRDMMEEIVKVHFDKVEEHLLEQVMTTFYWIR
                     SLKDIQKKPSTSELIDWIQALTLSGMPIEKIEKEVPFAGILLKNNEDIESMQRHL"
BASE COUNT         8901 a         4921 c         2789 g        10843 t
ORIGIN      
        1 caaccaatga aaaaaatcaa tattttaact attttcatat aaaattcata aatatatgtt
       61 ttttgttttt tatatattat tttcttttag tattttataa aacaaatagc catatacttt
      121 attttaaata tatggctatt ctataattta atatctaagc tgcttctttt attgaaactt
      181 tagttatatt acttattcta tacatttctt tgacaccatt tagagtttgc agtaagttta
      241 gacatattat cttgtcacct agacttttag tatttacaat actctctaaa attacacttc
      301 ttccttcatc atacttatct acattgtaat ttgaaatata attagtatct ctcaatattt
      361 tttcaacatc ttcatctttt attttatttt tattaacatt tatatataga ttatacatat
      421 tacttattag taatttctga ttttctaaaa aatcctcttg atgtttatct ttatatatag
      481 catttatcaa cataagtcta tcttcaataa gtgatggtat caataataaa ttttttatat
      541 agtaaacatc agctacattt tctatagact tattgacttc atcaattata tttttaacta
      601 ttcctttata catttctata acatgttgat agcctgtttt agcatcatat ggtaatataa
      661 acttatttac tattaatgca ataattgctc ctaatgctac atatgaaatc ctgcttatgg
      721 ttaaaacatt tgggtctccc ataactactg ctgaaccaag agctgaaaca gttacacata
      781 ccattaattt tctataatca accacataag agtttatata ccctgctaaa agaactatta
      841 acgaccttaa tgtactatct tttattattg aaaataatac aataaatatt acagctccta
      901 taaatgtacc ttctattctt tttctagacc tttgtataca attttctgcg taaggttgtg
      961 ttaatgaaaa gacagttaac attatccatc ttccatctct taaatggaaa taatccataa
     1021 taaaacccgc tacagctgta gctaaagcaa tctttactgc ataagaaaat cttatagaat
     1081 ttaaattaaa gtttttctta tatattgata gtcgtttaaa ttcatgaggt atctttgatt
     1141 ttctttcagc tttcttttcg tctcttgatt ttactttttt ataatctact aaaaactcat
     1201 ataaattttc taaaacacta taaatttctt ttaaatttat atttttagta tcctgagcaa
     1261 taacataatc tctaaatcct aatatctctt tatcagttat ggtatcttta ttttctaaac
     1321 acatcttaat atagtttatt tctttcgttg ctacaactaa aacatctgat ttactattta
     1381 tgctactttc ttctaaaata ttttttatat cttcatcttc aaattcttta ctatcctttt
     1441 tgtatctttc caggattaaa cttattcttt ctaatgaaaa taaaatattc attatgtcta
     1501 tacctctaat actgatataa aagtcatctt ttctattatc aaaaataacc attttcaaat
     1561 ttttagatat agaatttatt ctataattac agctatctat ttttctcata ctagatttaa
     1621 ttatagaact atcatcatta atagtatttt ttaaaagact aattttttct aataattcat
     1681 cacatataga tatcagttct ttatctccga catttttcat cctatttcta ttcataacaa
     1741 attgagcaat cattattata actgctccaa cagctaaagc cgacaatctc ataggaaatt
     1801 cactttttcc tacaggtata cttaccataa atatatacat tagaccaaaa ggtacatata
     1861 ctgctctttt taagtcataa ctaaatacat accctattat aaataatgct ataaaattta
     1921 ataaaactcc taaatacata ttttgtactg caaaaaatga tagtattcct aagattatat
     1981 ttattgctaa atacttcagt aaatttttaa ttggagaaat agttaaatct ctttccaaga
     2041 gtgttagcat agctgttact atacctacac ctataagtgt atttttactt ccaaataaat
     2101 atataaatat agaaataaat gctattataa aaataaacaa ctttgttttt gatattacta
     2161 gtttctttgt catcatatcc cccctaaaaa tttcaacaag accaatttta tcatatttgt
     2221 tatcttattc aaaatgcgct tttttacggt aggactttaa ctttttttat atttagaact
     2281 atataattca tatataaacg tattttttat atatataacc gatatatttt cgtatgctcc
     2341 catatttttt ttgtttcaaa tattttttca aaaatataaa aataactctc tcaataatac
     2401 taaaaatgca tttatcaaga gagttataat ttatacaata aatttaatta agcttttctt
     2461 tacctaatat ataagttcca agacccataa ataaagctcc tccaacaata tttccaagtg
     2521 ttacagctac taaattataa atagctcctc caatagtaac tgtacttatt gttggtgaaa
     2581 ataatgaaac actataaatt gtcatatttg ctacactatg ctcaaagcct gatgttataa
     2641 aagcaaataa acataagaat atcataatta tttttgcagt atcactatta gttctaaacg
     2701 aacacaacac tgatacacat actagaatat tacataaaat ccctctaaag aaaagagctg
     2761 taaatggcat ggatgcttta ctagctgctg tatttgcaaa aaattcagct acaggtcctt
     2821 tgtctactag accagttcct acaaatataa tgcctaaaac caaagctccg attaaattac
     2881 ctacccaact gtatgcccat attttagaag tatcttttat acttacacct ttatttaaca
     2941 taccagcaga cataaccata ttgtttccag taaataactc tgttcccgtc attataacca
     3001 aacttagtgc tattgcaaat gataacccca ttactatctt agtcataggt gaacctgcat
     3061 cagtcaaaag tcctccaata gtaaatatca aaagtatacc tatacctaca taaagtcctg
     3121 caaacgcaga agataccaaa tattttactt tacttgtgtt taataaattt atcttattta
     3181 tagctgcatt agttaattta tccaaagttt ctttatgcat aactccctct tccttctatt
     3241 aaattttaat ttattcaaat atatgtttta ctcaatttat agatatttaa ttccactcca
     3301 gtatatgttt tcgtacattt tagttatttc tggaagctct acaccttcaa gtgcccattt
     3361 tttaaattcc ataaatcctg ttctatcaat tatatatcct atatgttctt ttcctcctgg
     3421 tgcatcttta tctatatact tttctacata tctataagta ttaagtatta tctttattat
     3481 gctgtcctca tctacccata aaagaaaatc ttctgctagt ctagggtttt tctttcctgt
     3541 tctacccatt atagctaatc tatagtattt tttctcatct ctagtccatg catttgttgg
     3601 gcaatttagt acacactctc cacagcctat acacttatct ttatctctta ccaccttatt
     3661 attttctgtt tttaatgctc caacagatat cttagaacat ttttttacac atgctccaca
     3721 atttacacat ctatcctttt catatattgg taaagccata ccaataattc caaagtcatt
     3781 cattctagct tttatacaat catttggaca accagttaaa gccactttaa aatgtaaatc
     3841 atttggaaat atagcttttt ctattctttt agcaaattca gttgtattat attgagcctt
     3901 tggacagact ttatttccta tacaagctgc tatatttctt gtacctgctg ctggataacc
     3961 cgcgtttttc tcactttgat ttatattcat tttttcaata acaggttgta taattttatt
     4021 tatttcttcc atatcttcca tatttattcc taatatttca aacccctgtc ttgtagttat
     4081 gtgcacctgt ccattgccgt actcacttgc aattttagaa actacagcta aactctcagg
     4141 gtcaataaga cctccaggta ctctaactct taaagaagtc tcatattttg tttttgtaat
     4201 tctataagca tttttcatta cttttttggt atttaaatct cttatcacaa acttcacccc
     4261 ctaatcaata agatttttag catatgaata atcaaatact ggtccatcta aacatatgta
     4321 agtatcatcc attttacaat gtccacattt acctattcca caacacattt ttctttcata
     4381 agaaacccaa atgttctttt catctaaatc tctttttaaa aattcttcta ctgtaaattt
     4441 catcatcatt ggaggtccaa ctacaactat agaagtattg tttatatctt tcatatccaa
     4501 ttctggtatg tattttgtta ctaatcctac attaccacta tatccttcct ctgctccatc
     4561 tacagtaact aaaatatcaa gttttttact ccatctcttt aaatcatctg caaataaaat
     4621 atcttttggt gatttaaatc ctactattaa tttaaacttt tcgcattttt ctttattatt
     4681 ataaaaatat tcaactattc ctcttacagg ggctaatgca cttcctcctg ccactactac
     4741 aatttctctt ccttcataaa gggatacatc aaatccattt ccataaggac ctcttataaa
     4801 aaaagaatct ccttctttat aattaaacaa ctcgctagtt acttttccta catttcttat
     4861 tgtaaagtca atataatcct ctccatatcc tgatactgat attggacttt caccgtattt
     4921 aggtatagat atctcataaa actttccagg caatacacct tttgtattgc agtttactct
     4981 aaaagtccat tctttatcag tatgtttaac tatatctagt atttttgcac ttactggtat
     5041 atatgggttc atttatactt cctccttact aattactaaa tcatttattt tttctataca
     5101 ctttgaaaat gatatgtatt gcggacaggc atcatcacat cttccacaac caacacacat
     5161 atgatatccg aatctttttt taaaatcatg tattttatgc atagttttaa atctcattct
     5221 ttcaccttgt ttttttctaa aagaatttcc acctgctata tctgtatatc catctacttg
     5281 acaagaagcc caaacccttc ttctctcacc tacattttcg ttttccttat agaaaatatc
     5341 ctgcattgta aaacatgaac atgttggaca tacaaaatta catctaccac atcctataca
     5401 tcttgtatca tattcatccc atattgaatt tcctatcatt tcatttgaat caatattctc
     5461 tggcacatct acagatatta aattatcagt tacataatca acattaaatt cagttgcttc
     5521 tccattaaat acttcaaatt cactatcctt tatatctaag tacagtgtat ctccttctat
     5581 atttaatcca attgaataat tatcagtttt gttagaatcc atgctcacac aaaaacagtt
     5641 tctaaaactt tccttacacc ctacaagtgc aaactttact ttatctctga tatttttata
     5701 aaaataatct ttttcttcac cattatttag atatatttca tcaagtcttt ttataccatt
     5761 tatgtcacaa gctcttaaga aaattaaaat tttcttgttg tctaaatcgc tttccttaaa
     5821 ctctttttca gtaaaataaa acaatacttg atttatggga agtacaactt cttttggtga
     5881 aaagtttgct ttcttagcaa attccatttc ttcaaaagcg tttacctcct tgtatcttat
     5941 aacatcagta tctgaaaaag tacctttaaa tgggattaaa actggtgcaa atatcttgta
     6001 gtcttttttt agataattca gaccttcatt gaacttagat gaatctaatt ttagtttcat
     6061 ttttatcacc tttcttatat tttagtttta cttacttgct atcttgttta aatcttaaca
     6121 ctttattttc tatgcttccg tgataattat cacagtttca aaaataataa aaaaaatatt
     6181 aatggagttt attaagttct gttaatattt ttttattatc tacattattc gatttcaaat
     6241 ctaatcactt aaatacaatc attaagtaca ataaaactac tatctactca attttttgaa
     6301 taaaaaagaa tcttatttgt ttttttatac aattcaatag ggtaaaataa aaattaataa
     6361 attagacaat aaaaaagaat ctataaatag attctcttaa taatattttt atttaattaa
     6421 aattctaata atactcttac ttaatcataa ttaattattt ttaaaatacc ttgataattc
     6481 agtttcttta acataaaaac ctttattgtc aattttaata agttcttctt tttgcaatac
     6541 ctttatcatt ctggaaacag tctccctttt acagccaagc atatcagcta aatatgtaac
     6601 tgtaatattt acatttagta aagtccattc tccacagcaa actccaaatt ctctacttaa
     6661 cctatataat tttgctgcaa gttttttatc tacttttatg gatatagaat ttttcaactg
     6721 tctatataaa cgtcttattc ttctttccat ataagaaata atatcctttg taagttgaaa
     6781 atcattttgc ataatttcta aaaattcttc aatactataa taagctacaa ttgaattttc
     6841 aaatgcttca cagccaacag tagagttttt agaagcgtca agtgtaatat catttatgac
     6901 ttccccacta tttaatatga aaattacttt tctctctcca ttttcactta tcttaaaaat
     6961 agataccttt ccttctaaaa gtatataaac actgtctaag ctatcttttt cataaaataa
     7021 aatttctttt ttttgtaatc tttttagttt aactttttct ttaagttgat tcttcgtttt
     7081 agtacttaaa ttattaaaaa ttattacttt atcaatatta taatctttta atttagacat
     7141 gtaatatctc tccattccta aatttttaag agagctctat aaaacattac aaatttctta
     7201 ttttattcat ttttaaacaa ttttatagca tttgtagttt taattttcct ttcaatagct
     7261 tcattagaaa gttcttttac tacctgtgta tatactaaat caataatgaa aaattgtgct
     7321 aatttagaag atattgaacc ggtttccagt actgtttctc ccgatacata agataagtgt
     7381 acatctgaaa tatcctttaa ttcagaattt ttattttcag taattgatat aattttagca
     7441 ttattggctc ttgcaagctt tacagttttt ataatctctt ctgtttcacc agaatgtgaa
     7501 attgcaatta ttaaatctcc ttcttccatt atggaagaca tcattatcat tgtatgacta
     7561 ctgtcaaaac ttacacaatt aagacctatt ctcataaatt tatagtttga atcctgtgct
     7621 attataccag aatatccaat acctatgaag taaactttct ttgcatttat tataagcctt
     7681 gcgcagtcat gtacatcctt actattgatt atctctactg tgttctctaa agtggttaca
     7741 tttgaactta aaagtttttt tgctgtatcc aaagctggct catcattttg tatattttta
     7801 tttataatat tttttttatt gatagttgat atttcttgtg ctaaagtaac tttaaaatcc
     7861 tgtaatccat taaaattcat tttttttaca aacctagtta tagttgcttc accaatattg
     7921 ctctctttag ctatttgtga tattggttta taaaatacat catctatatt ttcttttata
     7981 taagctatta ataatttatc tgattttgtg actttaaaag ttggtgattc caattgttct
     8041 aaaatactca ttaaaaaccc ttctattctt tatattttgt ttaaaaaatt atataatgct
     8101 ccaagcattc ctgcatcatt ttgtctcttt gcaaattcta tattagtgtt tttgtaaact
     8161 ctctctataa gaacctcctt taatgctttg tctatttttg gttttaggaa cttttcttgt
     8221 gccataatcc caccacctaa aattaccact tctggattta ttacataaca tatatttgcg
     8281 attccaactg ctaatgattt tatcatatta tcgagttctt ttaaacaatc ttcatcatta
     8341 ttctttgcta aatcaaaaat tatttttcca tttaagtcat tttcattcaa attttttatt
     8401 tttgctactt ttttaattag actgctagta gatgccaatt cttgaaaaga acttccattt
     8461 acattcatat atcctacttc accagcacta ttactaaacc catttatcag tttattattt
     8521 attataatac atccaccaat tccagtacct attgttaaac atatagaaga tgttgcatct
     8581 ttacctgctc caagccacat ttcaccaagt ccagcacaat taacatcatt ttcaacttca
     8641 cattttatat taaactcttt ttctacttct ttttttaatt gcatgccctt atagtttgga
     8701 attaattctt ctaatgcaaa tagtatttta ccttcaactg ggtcaaccat acctgcagtt
     8761 gatatacata taccacttat ttcatttttc tctatatatt ttcttactat atcttttatt
     8821 ttggctaata ttccttctcc acctttttcc tttgcttcag tatcaagact ggatttatca
     8881 attattaatc catcttctct aataaaacca tattttattg aagtacctcc aacatctata
     8941 caaacgtaat ttttcataac atatctccat acaaattatt tttataaatt aacaacccta
     9001 tttaaaattt tatattacac aaatctttta cttatctttt ccaattcaca gatatatttc
     9061 cttgcttctg ataaatctat agtatcactt aatacacttt tttctttggt tatatgatac
     9121 ttataaatat ttttacataa gtcctctcct aacatattaa gaccttcttg agtgtaatga
     9181 acatcatcaa tagttaatcc tctgtcatga aattgcatag caattttact tcctacacac
     9241 agtatatcac attcattaca agcagattct tgagctgctg caattcgtct tgttctaata
     9301 aaatattcat cttctccaag ccaataacca gttaaactta caaacatttt atcaatcata
     9361 cactcttctt taagcctata cgcaattttt tttagacttt ctttatagta caatgcatca
     9421 ctaccatatt ttgcatcact ttcaccttga atccaaataa cgtatttatt ttctatatta
     9481 taatgtttac ttaaggtagt acagcctgca ttaaattttt ctatagtgtc atttagaaac
     9541 cagttattat catgattcca gttttttatt cttgaaccat cttttgcatt gtgaataaaa
     9601 caaactttat ttccagtaag cttattccat tcatttgaaa aagcaggtgc tatagtgcca
     9661 tcaccttctg aagtctctag ctcacatctc atatttataa tttcatcagt actcaaatat
     9721 tcaaaacaat tttcatttgg aatgatgctc tcttttggat ttcccaatcc tctagcattg
     9781 ctttgtccta ttaataagaa tacatctata ttttccataa gttaactcct ttacaaaatt
     9841 ttaagctttt aattattttc aattttctca taaaaaacta aggctgaaaa tagtaatcta
     9901 actaaaattt acgaagtctc actaatcgct gaaagttaaa tcactatttt cagccctagt
     9961 ggttatataa attatcatta tctaaccaaa accttaaaaa tagctttttt gatattttct
    10021 ttttctaaaa cttttattcc tgtcatatga gcatcttctg gataacaaac taaaaaatca
    10081 ccttttctaa gttctacgta tccattattt ttaccatcaa gaggcaagaa atcgccctct
    10141 tcttcatata ttttttgttc taaattttct ataaaattta tatctattcg ttcacatcca
    10201 tcaagcatca aatgaacatc tatatacttc ttatgagctt cccagaatct atcttctttt
    10261 tcacaagttc tatactctac aatattcaca aaaacatcat caccatctat tgtataagta
    10321 cctttttcat aatctattaa tgaattatct ttagcatatt caaaacatct aagtaaatct
    10381 tcatgcaatg ttgaatatgt cttactatga tttatatttc caaatatcat aagcactatc
    10441 tccctatata attataaagt ctatttttat acttttatat acaaagtata ttatacaatc
    10501 ttatagagca actaaattat ttatctaatc actctataag acaatttatc atttaaatat
    10561 tatttttcat aataaacagt agtctcagct tttggtaaag atacttttgc tttaacttta
    10621 gcttctattg cactaactat tgtacctacc actacagaag tcgtgattgt tattagagaa
    10681 tatgcccaag aagttacttc tggcatacgg tattttaaat aaacaactaa aattgctgat
    10741 actatgaatc caacatatgc tcccatttta gttgcttttt tacaaaatgc accaagtaca
    10801 aatattcctg ctaaaacacc tagtactagc cccataaaac tattgaacca ttcatatgct
    10861 gactttattt ctccatttgc taatactatt gcaacagcta tagaaagtat acctacacct
    10921 aatgatatat attgtgctat cttagtttgt ttttccatac ttatatcttt tgttatgata
    10981 gtttgtatgt ctaaagtcca acttgtagca actgagttaa gccctgtcga taaagttgat
    11041 tgagatgctg catatatcgc tgcaagtagt attcctgtta tacctactgg taattcataa
    11101 gctatatatg atgcaaatac ttggtcttgt tgaactgttt gagctaaatc tgggttttga
    11161 gtatagtata taaataaaca agttccaatt agataaaata ctgttgcaag tcccattgat
    11221 aaaacaccat ttccatatgt cattttatta agttgcttta tatcagttgt tgtagtaaat
    11281 ctttgaacta tgtcttgact cgatacatat gatgaaaaag tattaagacc agctccaaca
    11341 aatattataa atgcacttgt agatagtata tttttatcaa acataacttc attacttgct
    11401 aagaacttgt gtccagttgt aagagtatcc attactgttc caaaaccacc atttatactt
    11461 gctattaaat agaacaatgt ccctgtaaca cctactatta ataccattcc ttgtataaaa
    11521 tcggtccaca agacagcctt taatccacct gtatatgagt aaattattgc tattacaccc
    11581 ataactatta tcagtacatt tacaggtatt cctgtaagac cagccagaac tattgatgga
    11641 agatacatta taatagacat acgtcctatt tgatatatta taaacatcaa agctccaagt
    11701 actctaagac ctttactttc gtatctcatt tctaagtatt catatgctgt atctatttct
    11761 aacctactat atagaggtaa gaaaaatttt attgtcaaag gtattgctat aaacatacct
    11821 aattgtgcaa accacaaaat ccaagtacca ccataagaat ttcctgctaa tgataaaaat
    11881 gatattggac ttaataatgt ggcaaatatt gatactgatg taacccacca cggaatagtt
    11941 ccatcacctt tgaagaactc ttttcccttc atttcctttt ttgaaaataa caatcctgca
    12001 aataaaactg ccaataagta aaccactagt attgcaaaat ctatccaagt aaatcctacc
    12061 atttgactaa tcctcccata ctaaaagtat tttattaaaa atactttctg tatatttctt
    12121 ttgctcttga tttcatttca tcagttgctt ctttcatagg ttgtctacag tatccagcct
    12181 caacaccttg ttcttcaagt agtaatttaa ttgtttgata taagccattt cctaatatgt
    12241 ctgttattaa atcattagtc acatgttgta cttcaagtgc ttctgatatt ttttcattct
    12301 tagttaattc gaatatttgt ctagctctga caccgtttac attgaatgta gaaccaattg
    12361 ccccatctac acctaaaact gtagctggta acatcatttc atcaaatcct gcaaatatta
    12421 atttatttgg gaatgttttt ctcattctct ctaataagta gaaatctgct gctgtaaact
    12481 taacacctat tattttttca ttttcaaata attctccaaa ctggtctaaa ctcatatcaa
    12541 caccagttaa aaatggtata gagtatatta ttaatctatt atctacagaa tttattattg
    12601 tattataata gtgttttatt tcttcaaaat caaatttata gtaaaaaggt gtaacagcac
    12661 ttattgcatc atatcctaaa tcagttgtaa atttagcaag ttctacagct tcttttaaat
    12721 taactgaacc aacttgagct attaatttta tttcttcttt cacttcatct ttagctattt
    12781 caaatattct tttcttttca tcagttgaaa gcatgaagtt ttcccctgta cttcctccta
    12841 cataaagacc atctacttta caaacatcta tattgtgtct aattatttgt cttaaccctt
    12901 tttcatttat atttccttcc ttatcaaatg aaactaataa tgctgaataa attcccttca
    12961 tatctgttgt tctcatatct ttacctccaa ttttaatttt caaatatagt taaatataat
    13021 aattttttaa tttataaatt aatttttaat taatttcaat tgctttaaca aatttttctg
    13081 ttattaattg tggtcttgtt attgctgaac ctactactga tgaatgaaca cccaactcaa
    13141 agaccttttt taaatcttga ggagtattta cctttccttc agctattaca ggtatttcta
    13201 aatctttaac taatctttcc attaatacaa aatctggacc ttctaatgtt ttagtatatg
    13261 gagtatatcc agatagtgta gttgaaacgc aatctacacc gtactcttgt gctttgatag
    13321 cctcatcata atttgaaata tctgccatta ctagaacacc gttttctttt atatacttta
    13381 taagttcttt taagtcttca ttatttggtc taactctatt ggtagcatct aatgctatca
    13441 tttcacaccc tgttgttaaa agttcatcaa cttctttttt agttggcgtt atatatatat
    13501 ctgaatcttc atagtttctc tttataatac ctataacagg aagtccagta acttttttta
    13561 tttcaataat atcttcaact ccttgagctc taattccaac agctccacct tccatagcag
    13621 ccttagccat tctccccatt ataaaaggac tatgaagagg ttcatcttct aaagcttggc
    13681 atgatactat taatcttccc tttaccttat ctagcatttt ttaaattacc tcgtttctga
    13741 aatttatttt cttgatttat gtattaataa taacatgaaa actttttctt tgcaatattt
    13801 tttcatattt ttttctcgca tctatatttt tactgattta ttttatatat tagcttgttt
    13861 tttacattta aaagaaattt tatttcattt ttttggtgtt tattttcatt ttatataact
    13921 actgttataa gtccatttaa ataatttcaa taaaaaaacc catgtatact atacataggt
    13981 ttcccaatat tattctaaat ccattataat ttttcttcca cagcatggac aaaattcagc
    14041 ctctgtatct atctcacttt cacaataacc acaaattata aaatcatcac attcattttc
    14101 caattcaaat tttttaccac attcagcaca atatttatca tttacatttc caacataacc
    14161 acactcacaa gttacattac tttctgccat ttccatttca ttaatttcca tatttttttc
    14221 ataaattaac ttatcaagtt ctagtaactc atcacagaat ttatcaaaac tattatcatt
    14281 aatagaatct tctctaattt tttgatatgt cagtattccc atttccaaga atatctttgc
    14341 tttcttttct tgaatatcat caatctctct atttaatttt gatttttctt tatataattc
    14401 aactttaaat tttccactac caaacttttt cttaacctta gaaaatttat catctacttt
    14461 attattagta tttccttcca cagaaattcc ctccaatcat atttaatcag ctgaatttag
    14521 cttatatcct attaaagata tcacaaaact atatacaaat gaaattacaa tagcttgtaa
    14581 gactttaaat tgcattatta aagttgttcc ataattagtt gtattaaaga aattaatatt
    14641 actatctagt ataaatgtac taaataaagc caatattccc atcaaaaatg cataatatat
    14701 actaaatact attataggtc ttatactatc tgtattatac ttaaatctta gcttgtatcc
    14761 atttaataat aatattaaca tagacaatgc tcccattgca taaaacatta atttagtatc
    14821 tccaaataaa gatgaagaac ttaaagtaaa cattgatact gttgagttta ttatggttac
    14881 tgaaattcca tttgcaaaag cccacatata actagctatt tgtgggagta tagtaaataa
    14941 atttaatcca tttgaataac tactcatatc tagttcattt aagtaactac tatcagatat
    15001 agttagtacc aatagtatta ctaatactaa aacataacct aatatgaatg ttcttataga
    15061 atttgaaaaa agagataaat acatattttc tttttcataa ctttttttat aagtagttat
    15121 gtatgtacat ataaacccta atacgaatcc atttaaaaga acactaaaaa attcataact
    15181 atattctaat gcatatccat attctaacat actatgtgaa ctagttttta cattagtaaa
    15241 aatagataaa atagctaaaa ctaatccata aaaaattcca actcctagag aatttgctaa
    15301 tacagttttt gaatctctac atctttttct cataaaaatt aggtttgata ttaataatac
    15361 caaaataggg attataagta atatcaacaa acctatattg gcatttataa acccagaacc
    15421 catcatggta gacatagata cactaatttg acccaagttg agagctagga ttatatgtgc
    15481 tggatttact aaataactaa tatcattaaa ttgaatagtt ataatagctt taaatatcaa
    15541 tgcaataata aacaatataa atatggttgt aaatgttgtt aaaaatacac ctcttttatt
    15601 tgcatgatat gaaagctctt ttattttaga tttcaagtct agttttgttt caagtggtct
    15661 taatgatgct atttcataaa gttcatttcc acaaaattta cagtaattat ccttagtatt
    15721 attttcttta ttgcaactag aacaaatttt ttcttcttta cttttaagag tgatatggct
    15781 attttttatt tttctagttg gttcacctgt ctttttggaa taattattat gtgaataatt
    15841 catattattt attttctttt cacccatttt ttccctccaa gttttactat tgtaaattat
    15901 caaatataac tatatcatat aaaacttaat tataccttaa ttggatatat ttatctattt
    15961 tatttgttta ttgctgttgt tttttaacag ttcttgatat ttaaatagtt tgttaatgcg
    16021 aactttatta tttatatttt attatgctta atttaaattt attctattct ttattttcag
    16081 tagtaaacta tctgtaaatc ttaattataa taaagtatta atcaataaag ttactcgaca
    16141 ttatctaata aattttatct atcacaattt tagattgtta cacacaatat attttgatat
    16201 aattaaatca taaacgaagt attccaaata tctaatgata gaaaggaata cttattttta
    16261 tgctttattt ttaatggagg cttatatgaa cttagaaaaa ataaaaaaat tttttggatt
    16321 tcctctagac aaaaaacaca aagacaatgt tgagaaagaa ctctgcaatt atctttatca
    16381 tacgggtaaa atcatttgca taataatagt aatatctcaa ataattatga tgatatcaat
    16441 ctcagctaga gaaggaggcc cttttgtcac accaagacgc caatggtatt tttatatgta
    16501 tgtaacacta ttttgtgtat cttctgtctt tatgtttgta ttttcttacc tttggaataa
    16561 aaaaaaggat tgctgtacgt tttatataaa tttatggata atttatgtga cttttttctg
    16621 cctatggggt tgtgtaatta ctctacttga ccagttaggt ggaaatgacc taagtgtatt
    16681 tagttatatt atgttagcag ctgcagcttt ctcagtatta aaatttggag aatcagtaat
    16741 agtttttgga ggatgctttt tgtttcttaa tttattctta cctgggattc aaactatacc
    16801 aggcaatgag tttagtaata ttgtcaacac attttttata acatcccttg ccatttttgt
    16861 ttctacaatg ctatatagaa atcgtgtatt tacttactat gataaacttt taattagtca
    16921 acaatatgaa gaaatatctc atataaataa tttacttaac gacaaagtta tgacagatga
    16981 ccttactaaa atgaacaacc gtagatacct tgaagatatt ctacaaaaaa aattaatcag
    17041 ccatgcaaat caagaaccta taactggaat gatgatagat atagattatt ttaaacaatt
    17101 taatgataat tatggacatc aaaagggaga ctattgtcta caaaatattg ccaaagtaat
    17161 tttagatttt acaagttctg aaaatgaaca tgctatacga tatggtggtg aggaattttt
    17221 tatatgtatt tttaactgta atgaggcaga tgctaaactt aaatcagaat gtttacgaaa
    17281 aattatagag tcctgcaatt ttgagcgtga tgatgttcct ttaaaatgtg taactgttag
    17341 tattggagtt tatacaaaga aaattggaaa gacaataaca ttaaatgagt ttataagtaa
    17401 ttgtgataag gccctatata ctgcaaaaga acaaggacgt aattgtgttg cattttatta
    17461 aaaggttaca tactattaca taaacctata agtattatat cgcaaaataa aagtaatcaa
    17521 attatatatt tatagtacaa taaaaataac ttgcatatct ctaaaataga taaatatgca
    17581 agtcatttta gtgtttatac ttaaagttat ataagtttta ttatatataa ttattaaaat
    17641 tcagcatttt taggagttct tgggaaaggt ataacgtctc ttatgttaga catacctgtc
    17701 atatacataa ttattctttc aaatccaaga ccaaatccag agtgagtagc agtaccaaac
    17761 tttctaagtt ctaaatacca ccaataatct tcttctctta atcccatttc attaattcta
    17821 tctaacaata catcttctct ttcttctctt tgagaacctc ctacgatttc tccaactcca
    17881 ggaactagta aatccattgc tctaacagtt ttaccatctt cattcattct catatagaaa
    17941 gcttttatat cttttggata atctgtaaca aacactggac aattatatat ttgctccgtt
    18001 atatatcttt catgctctgt ttgaaggtca catccccact caacaggata ctcaaattca
    18061 tgcccagatt caattaataa ttccactgct ttagtatacg ttattctaat aaactcagag
    18121 tttactatct tatttaatct ttctaacaat cctttatcta taaaactatt aaaaaattcc
    18181 atttcttcag gagcattttc taatacatag cttataacat attttatcat atcttctgca
    18241 agttccatgt tatcttcaag gtctgcaaaa actatttctg gctctatcat ccaaaattct
    18301 gaagcatgtc ttcctgtata agagttttca gctctaaatg taggtccaaa agtataaacg
    18361 tttctaaaag aaagtgccat tatctcggca tttaattgac cactaactgt taaattagcc
    18421 tcttttccaa agaaatcttg agtatagtct attttgccat cttcagtttt tggaatatca
    18481 tttaaatcca tagttgttat tctaaacatc tctcctgcac cttcacagtc actacctgtt
    18541 atgattggag aatgtgcata tacaaagttt ctttcttgga agaatttgtg tattgcatag
    18601 gcagctattg atcttactct aaatacagct gaaaaagtat tacttcttgg tcttaaatgt
    18661 gctatcgttc ttaaatattc aagcgtatgt ctcttctttt gtagtggata cgaactatct
    18721 gattcccctt ctacaactat gctttttgca tgtatttcga ctggttgttt agccccttct
    18781 gtagatacta actctccctc tacaagtata gatgagctta ttgctaattt tcctacctct
    18841 ttaaaatttt ctactttctc atcaaatact atctgcatat ttttaaagaa gcttccatca
    18901 tttagctcta taaatccaaa atttttagat actctagatg ttcttatcca tccagctact
    18961 tttacagtct tccctatgta ctgttctttt tctctgtaca attcttttac tgttataaat
    19021 tccttttgca ttttaaatcc tcctagtata attgttatta atttctttta aatataattt
    19081 caataaaaaa agcccttcat ccctatataa agggacgaaa gactattact ctcgcggtac
    19141 caccctagtt gtcataatga cctcttagtt aagcaatgct taaagcctat aacggtgcct
    19201 tccgtctaag tctacttact aaataagctt tcgattagaa actccaagat gttcttcaat
    19261 ataaacctcg tattgagctt tcaccatcct caactcgcta gaactacatt ttatatctac
    19321 tcttctcttc acagtattta tcttttatta tttctgctaa ttaacaaaat tataccaatt
    19381 tataaatttt tagtcaataa atttatataa ttgatactga ttgttattta aaatataata
    19441 attctacctt acataatgat aataaatact tatcttcatt acttctttat tacttcaatt
    19501 tccaaaattt tataatttac ttccattgtc aacccagtct tttgcttctt cgacaccctt
    19561 aattgaaggt attgaaactt tactatgagg ttttaatttt tcaacatcag cccatgctgc
    19621 tgtcgactca tttccaacta aaagtgaact tttaagtctt ttattatctt taccttttaa
    19681 tgctcttgcc tttttacttt ccaaaactat cacctctatt atttattttg taatagtttt
    19741 agcaatttta caaataaaat tcataagtct aaattataat tatttttaaa ttaacatcat
    19801 atcttttcaa tacttaattt atacctattt atatacaaaa tatttacttt gtgtattcaa
    19861 aatataaaac tgttttttaa ttttcatatt tattttttat gctatttttc tactctatct
    19921 aaagtatttg atacaattaa actaataatc aattataatt ttacataggt tcttatctat
    19981 agagtatttg ctatctgttg aatcgtattt tttaaattta aaatcccata tccctgcgat
    20041 acatttggat atgtatatat ctcaagtttt gtagcaccca atattaaata tgtttttaaa
    20101 acttgtgtaa acaatgataa tctcggtaca ttatcctgtt tttctaagta ttccatcaat
    20161 aaggctaaaa cacctgtgac tatagaactg ctcactcctg ttcctgttcc tgtattatat
    20221 gttccatttt tatatgtgga tattatatct acacctgaag ctactatatc tggcttaatt
    20281 cctcttcctc taattggtcc tttcgaagaa ccaatccaca tactatcagt cttattgtta
    20341 aatgtaccaa ctgtaattac atcatcacta gcagcataca tagttatagt agctactgaa
    20401 tcagggtcta aaaaccttgt gtctggagct atcaaatttt tatttggcaa gtatatgtca
    20461 tattctccat ttattataag ttctgaagtc aatcttaaaa tccatactcc aggttttata
    20521 tctcttaatc ttatctccaa attttctttt ccagaggtaa tatatggata aataaatctc
    20581 attgcataag tagtattttc taagttaaat tttcctctat atatataaaa gtctggagaa
    20641 taccttatat catgactaac ttcacctgaa ggagatatta tctgtgctcc cactttatct
    20701 ggaccattcg tatttagtgt tatatcaaga gcatagtcat ctccatcttg tattattaca
    20761 tcttgaactt ctcccacact gctaaatctg ccagaataat gaatatctgt attcccttga
    20821 tttcctgcac cactcactac taccacacca gcactactca atatattaaa agtatctagt
    20881 attgaagtag ttgctacagc acttgactta actccaatag tcaaatttat tatcagtggt
    20941 ttattttctg ttcttgctat gttagtaaca tacgttatag ctgctaaaaa atctgaaaca
    21001 ctgtaattta tccttccagc ataataagta tctgtatatg attttaattt aactactatt
    21061 aagtctgatt ctgtagtaat acctctgtac tgagaattta tccttccatt tccaactaat
    21121 ataccagaaa ctaaagttcc agtacctata ttatcttgac tcaaactccc atcatttcta
    21181 tttatggcta tatttaactc acttcttgta aattcacttc caaaaataaa accttctgga
    21241 ggtggatttg tatttgcttc ttggtcccat aaatataaaa ctttactagt tccatcatca
    21301 tttataaaat caggatgcaa ataatctatc ccagaatcta taactgctaa taaaattcct
    21361 ctccctgtta tatcattata tggattttca taaatataat cagtttcagc tgctgttgta
    21421 atggtttctc cattatttac attattagtt atttcaatta gactgctcat tggttcagat
    21481 tcttcccacc aagcaacttg tattatatta tttaaaatag tttcatcaaa atctaaaggt
    21541 acatatatta ctgcaagctg actatttaaa accatatacc tatttattcc attttcttgt
    21601 agggcagatt ctatatcacc ttgataaatt atacaataag atttttccat aaatccctcc
    21661 tatcttaaaa catcaaacat tcctctaaca tccaagagcc catatccaga atttgtattt
    21721 ggatatactg tgtttgaatc ttttctagca ccagcttgca taaaagtttt tattttttgc
    21781 acatatgctt ggtttggata tctaccgtca acaaaagtat attgaaaata cattgcagca
    21841 gcaccagctg catgtgcact tgctgctgct gtaccagtta tagttgcata cgtgttccca
    21901 ggataagcag ctattatatt gacacctggt gctactatat ctggttttaa cctgtcttca
    21961 atagttggcc ctcttgatga actttgccat aaactaccat taatagtatt gtaggctcct
    22021 atggttatta aatcatcttg aatcgcagga taatttatag tgtaaaaagg gtctacctct
    22081 ctaaatctag ttccactttt taaaagttct ctatttggaa ggtataagtt atatcttcct
    22141 gttataatat aaactcctac taatcgaatc ttccatacac ctctttttgc attctttaaa
    22201 gtaacattag taaattgctg tccagaaaaa gttgttgggt atatatatgt tatagaatat
    22261 tcagtcccct ctaaatcaaa taagcctgtt actttattat aatttgaaat tccaacactt
    22321 ttactttctt caccagtggg agatactatt attacatcag ctttatctgg tctatttaac
    22381 caaagttcta atgataattc ttcctcgtct tcatttaact ctaattcaac ttcaacagaa
    22441 cctccaacat atggtattat tccagatgta tgtgtctgtg tattaccctc atttcctgct
    22501 cctgccgtta tacataaccc tctagtaaag aacgcttttt cactattact cctattggtt
    22561 aaacctgcta ggctactggt acctaaagac atatttataa ctaatggtct tccaagttca
    22621 aaagcttttt tatatgcata ctgagatgca gcaaacaaca ttgcactatt ataaaaatca
    22681 tctatcttgc cgagttttat tattattagc tcagaatctt ctgctatacc tgcatattca
    22741 ctatttacat ttccaagccc tgcacatatc ccactcaaca tagttccttg accaacttca
    22801 tcctgagata gactagggtc attttctgcg atagctcgat ttatatcttc tcgtgtatat
    22861 tctgtcccaa tataaaaacc atcaggagga gttccttctt ttgtttggtc ccataaatat
    22921 actatttttg aagtgccatc tggataaata aaatctgggt gtagatagtc tattcctgta
    22981 tctgcaattg atattagagt ccctcttcct gttatattaa tattaggatt atttttaaag
    23041 aaatttacgc ccatttcttc tgttgcaact acacctccaa aagtacctcg attaatttct
    23101 ccaagtaatg tcatctttgt agtcgataca gttcttatga ttgaaggaag ttgcaaaact
    23161 cgttgtatac tactatagtc tgtattattt atagatagta cacctaatga atcacttatc
    23221 ttaaaaaacc tttgactcat acctaaccca gctagttctt cttcaaattc aggagtatgg
    23281 actatatcta ttcctgatag tattcctgat tgtctacttg catttttact tatataaatt
    23341 tcattctcta agtcattatg tatttgcata ttataaccat ctataaactt ataactattt
    23401 tcctcatctt taaatgactt attaatactt ctatatccca tatcctctgt ttctaaatct
    23461 tgatttatat tagatagagt gtacaaatct atatctgata aatttaaaaa accaaatcct
    23521 gatgaattat ttggataaga ttgattactt aatctccttg ctccctttag aagtaatgcc
    23581 cttaatttct gtgaatacaa aaataaatca tttccattta caattcccca ttccataaac
    23641 aatgaacaaa cccctgtaac atgaggtgta gccatgctag ttccagttaa tgctccactt
    23701 gtacctcctg gtaaaaaaga tactatatct tcacctggag ctaataaatc aggtttaaaa
    23761 acacctaatt gtgtatctcc ttcaccagaa aatatagata ctatatctgt tcttgaatta
    23821 aaactaccta ctgttataac tctacttgct gttccaggaa ctgttacagt aagctcttga
    23881 gtaggaatca gaaatctagt atttctattt agaccttcag aagttggcaa atatatattt
    23941 acattaccag ttactatatc tattggttca aatacaattt tccataaccc aggagttatc
    24001 tgtgtatttg aacttaattg tagagtaact cttcttgtga gagaatacgg tgctataggg
    24061 taaaaataac cagttattct agtttcaccc aaagtattcc ttatctcacc tgaagttaga
    24121 gagattgctt gtgtttgatt atttgatggg tttaccaaat gtacactaaa atcatcaaca
    24181 aaatctggcc atatatttat gtttaatata cgctctcctt ccccaactat aaactcaact
    24241 tcttctgtaa tattattttg aagcctaatc cttttatgac ctcctttatc tgcattattt
    24301 ccagctgcta caactatatt gtttttccaa aataaacaca tatcatctat atattgttca
    24361 aataaagatg ttcctctatg tgaaccttca ttacttccat aacttatatt taatgtaaca
    24421 ggcattctta attctaatgc cctatctaaa ataaatttaa ttgccctcat aaactctgta
    24481 cttctcgaaa aaatatcggt ctgtatattt ccaactctaa ccactattat tctggcatca
    24541 cttgcaatag ttgcacaaat acctgcaaca tgtgtcccat gtaagctagt tgttgaaata
    24601 ggtatataca tacttccatc tattgcatta tttatgtctt cattagtata taatgttcct
    24661 tctctaaaac cttctggtgg atttccttga atagattggt cccaataata taatatctta
    24721 gaccttccat cactatctct aaacacaggt agagtataat ctattcccga atcaattata
    24781 ccaattatag ttccttttcc agttaaacca gttctatttt taaatcctgt tattcctgta
    24841 cttgaaaaac tttgcacatc ctgagtctgt aatataaaag gcttttctat aaattcaatt
    24901 tctggataag ttaaaagtat atttacatct tcttcattac ttgatgttat tattgcatat
    24961 gatgaattaa gtatctctac tgatacacct aattcttctt ctaacctcaa tatatctcca
    25021 ttgtatttta caattaattc ataatttatt ataatcacct cgacttctaa aattattatt
    25081 aatgtatatg ttttgtgtga gtctaaaatg tattagacta tataattttt taagtttcag
    25141 ttatacaatt tataataggt atattaaaaa aataaataat gatggtacaa ttttgaataa
    25201 atcataccat catttatatt tactttatct tgaaaccatt aaatttttca atgctttctc
    25261 aagacctttt acagtcaatg gaaacatatt aaattcactt tgtatcattc tttgagtttg
    25321 ataccaataa ttaattgaat cccatccatc tctaagttct ggattcatcc atacaatttt
    25381 actgtacttt ctcttaaacc ttttaagcca ctctatacca ggtgtgtaat tatatggacc
    25441 acgatagtta ccaccaatat gaagcaactc agaaggcgac atagaagcat ctccaacaat
    25501 tataacttta tagtttttat ctatattttt taatatccat tcagtattaa tagatttact
    25561 taaccaacat tcaggtgttg taaataatct atcataaata cagttatgga aataatatat
    25621 ctttacatct ttaaaatgat ttgatttact tacagactga aataatgtat tacataaagt
    25681 gctataccct ctcattgaac cacctgaatc cataagcaat aaaagcttca ctgtattttt
    25741 tcttggcttc tcaaaaaata actttaaata tcctgcatta ttacaagtct cttcaatagt
    25801 cttatccaaa tcaagctctg tctttggagc atctattcta gtagaaaatt gacgaagtct
    25861 ctttagtgca acttgaaatt gacgcatatt taaaacttca tcatctctaa aatctctgta
    25921 ttttctttct cctgcaactt caagaacagc tctaccataa gtagaatttc cacctatacg
    25981 tataccagtt tgcccttttc ctgaatgtcc taactctgaa gtgcctcctg tgcctatcca
    26041 ataattacca ccattatgag gagaatcttg ttcttttatt cgttcctcca atttagctct
    26101 taatttatca agatttatat ttggattttg ttctaatctt tcaaactctt ctaaatctat
    26161 atctggtttt ttaagccaat tcataatctc ttctggaatt tcttcttggt gttctatacc
    26221 tttaaaatac tccaaaaaca ctgcatcaaa cttatcaaaa tcactttcac tttttattaa
    26281 aatcatcctg caaagatagt aaaaatttga aaagtttgaa tagcacagtc ctttatccaa
    26341 tgcttcaatt aaagtcatcc attcatttaa agatacattt aatcctcttg acctcaataa
    26401 ataaaaaaat tcaataaaca taattatcca gctcctatta actataagtg tctttgcata
    26461 gattcaatat cctcattatt cttaagtagt attcctgcaa aaggaacttc tttttctatt
    26521 ttttcaatag gcattccact caaagttaat gcttgtatcc agtctattaa ctctgatgta
    26581 ctaggctttt tttgtatgtc ctttaaactt ctaatccaat aaaaagtagt cattacttgt
    26641 tccaataggt gttcttctac tttatcaaaa tgtactttaa ctatttcttc catcatgtct
    26701 ctatcaggaa attctatata atgaaatatg catcttctta aaaaagcatc tggaagttct
    26761 ttttccgcat ttgaagttat aataactatt ggtctttgtt tagctcttac agtttctttt
    26821 gtctcattta tgtaaaactc cattttatcc aattcccaaa gcaagtcatt tggaaattct
    26881 aaatccgcct tatctatttc atctattaaa agaattactt gttgatttga gctaaaggcc
    26941 tctcctagtt taccatattt tatgtacttg ctaatatcat ctacaccttc tcctccaaat
    27001 tggctatcat aaagtctttg aacagtatca tatacataaa gcccttcttg tgcttttgta
    27061 gttgatttta tattccaaat tactaaatct ttttttaatt catttgaaat tgcttgtgct
    27121 agcatagttt tacctgttcc aggttcgccc ttaatcaaaa gtggcttttc aagagcaata
    27181 gcaacattaa ctgatgccat caattctggt gatactacat aatcactact tcctctaaag
    27241 ttagatatct ctttttttat cataaatcct actcctttta aacagtattt tctaaatgta
    27301 tatagacttt attactattt ttgatataaa aataataata caatacattt gtatagtatt
    27361 attctaaaat tctatatcta aataaaatca cctctaaaat atttaatttt tatctttatt
    27421 tgtcataatt aaatgaaata tatattttaa agaa
//
LOCUS       DBGJFF010000045        27414 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.045, whole genome shotgun sequence.
ACCESSION   DBGJFF010000045 DBGJFF010000000
VERSION     DBGJFF010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27414
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.045"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            <1..572
                     /locus_tag="ACOIAF_001904"
     CDS             <1..572
                     /locus_tag="ACOIAF_001904"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668915.1"
                     /translation="IAQNIRRLKMKKFIKVSFFILGIFTLFIAINKLVYRDMVSVKDY
                     EINHLEEIVFDFDSRRSRDVGTIVRFKISNNSKYSYKLNSAKMKFENCVENKKGMNKC
                     SAYVNVDLFNEKNSITERTLKYGIKPNNQGYVDFVIPKGLNFDYRYFNESGMSVEYKG
                     EYIVNIPMAKGLYLVVGKNNDTWNANIME"
     gene            651..1118
                     /locus_tag="ACOIAF_001905"
     CDS             651..1118
                     /locus_tag="ACOIAF_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HGZ2668916.1"
                     /translation="MSISNVREYFKQFGKEDSILEFEQSSATVELAAEAAGVIPARIA
                     KTLSFKIGDDAILIVTAGDAKIDNKKYKAEFNCKAKMLTPEEVLEFTGHAIGGVCPFG
                     LKNSIKVYLDDSMKRFDTVFPACGSSNSAIELTCEEMEKFSKCEKWVDVCKNW"
     gene            1827..2963
                     /locus_tag="ACOIAF_001906"
     CDS             1827..2963
                     /locus_tag="ACOIAF_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009272751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HGZ2668917.1"
                     /translation="MDLNSKKYQMLKELYVSFAENEVKPLATELDEEERFPYETVEKM
                     AKAGMMGIPYPKEYGGEGGDTVGYIMAVEELSRVCGTTGVILSAHTSLGSWPIYQYGN
                     EEQKQKFLRPLASGEKLGAFGLTEPNAGTDASGQQTTAVLDGDEYILNGSKIFITNAI
                     AGDIYVVMAMTDKSKGNKGISAFIVEKGTPGFSFGVKEKKMGIRGSATSELIFEDCRI
                     PKENLLGKEGQGFKIAMSTLDGGRIGIAAQALGLAQGALDETVKYVKERVQFGRPLSK
                     FQNTQFQLADMEVKVQAARHLVYQAAINKDLGKPYGVEAAMAKLFAAETAMEVTTKAV
                     QLHGGYGYTRDYPVERMMRDAKITEIYEGTSEVQRMVISGKLLK"
     gene            3022..3804
                     /locus_tag="ACOIAF_001907"
     CDS             3022..3804
                     /locus_tag="ACOIAF_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HGZ2668918.1"
                     /translation="MNIVVCIKQVPDTTEVKLDPNTGTLIRDGVPSIINPDDKAGLEE
                     AIKLKEEMGAHVTVITMGPPQADMALKEALAMGADRGILLTDRAFAGADTWATSSALA
                     GALKNIDFDIIIAGRQAIDGDTAQVGPQIAEHLNLPSITYAEEIKTEGEYVLVKRQFE
                     DCCHDLKVKMPCLITTLKDMNTPRYMKVGRIYDAFENDVVETWTVKDIEVDPSNLGLK
                     GSPTSVFKSFTKSVKPAGTIYNEDAKTSAGIIIDKLKEKYII"
     gene            3823..4833
                     /locus_tag="ACOIAF_001908"
     CDS             3823..4833
                     /locus_tag="ACOIAF_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HGZ2668919.1"
                     /translation="MGNVLVVIEQRENVIQTVSLELLGKATEIAKDYDTKVSALLLGS
                     KVEGLIDTLAHYGADEVIVVDDEALAVYTTEPYTKAAYEAIKAADPIVVLFGATSIGR
                     DLAPRVSARIHTGLTADCTGLAVAEDTKLLLMTRPAFGGNIMATIVCKDFRPQMSTVR
                     PGVMKKNEPDETKEAVINRFKVEFNDADKLVQVVQVIKEAKKQVKIEDAKILVSAGRG
                     MGGKENLDILYELAEIIGGEVSGSRATIDAGWLDKARQVGQTGKTVRPDLYIACGISG
                     AIQHIAGMEDAEFIVAINKNPEAPIFKYADVGIVGDVHKVLPELISQLSVAKEKGEVL
                     AN"
     gene            4852..5649
                     /locus_tag="ACOIAF_001909"
     CDS             4852..5649
                     /locus_tag="ACOIAF_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HGZ2668920.1"
                     /translation="MSTSDVKVYENVAVEVDGNICTVKMNRPKALNAINSKTLEELYE
                     VFVDINNDETIDVVILTGEGKAFVAGADIAYMKDLDAVAAKDFSILGAKAFGEIENSK
                     KVVIAAVNGFALGGGCELAMACDIRIASAKAKFGQPEVTLGITPGYGGTQRLTRLVGM
                     AKAKELIFTGQVIKADEAEKIGLVNRVVEPDILIEEVEKLAKIIAKNAQLAVRYSKEA
                     IQLGAQTDINTGIDIESNLFGLCFSTKDQKEGMSAFVEKREANFIKG"
     gene            5747..6592
                     /locus_tag="ACOIAF_001910"
     CDS             5747..6592
                     /locus_tag="ACOIAF_001910"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HGZ2668921.1"
                     /translation="MKLAVIGSGTMGSGIVQTFASCGHDVCLKSRTQGAIDKCLALLD
                     KNLTKLVTKGKMDEATKAEILSHVSSTTNYEDLKDMDLIIEASVEDMNIKKDVFKLLD
                     ELCKEDTILATNTSSLSITEIASSTKRPDKVIGMHFFNPVPMMKLVEVISGQLTSKVT
                     FDTVFELSKSINKVPVDVSESPGFVVNRILIPMINEAVGIYADGVASKEEIDEAMKLG
                     ANHPMGPLALGDLIGLDVVLAIMNVLYTEFGDTKYRPHPLLAKMVRANQLGRKTKIGF
                     YDYNK"
     gene            6679..7854
                     /locus_tag="ACOIAF_001911"
     CDS             6679..7854
                     /locus_tag="ACOIAF_001911"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HGZ2668922.1"
                     /translation="MREVVIASAARTAVGSFGGAFKSVSAVELGVTAAKEAIKRANIT
                     PDMIDESLLGGVLTAGLGQNIARQIALGAGIPVEKPAMTINIVCGSGLRSVSMASQLI
                     ALGDADIMLVGGAENMSMSPYLVPSARYGARMGDAAFVDSMIKDGLSDIFNNYHMGIT
                     AENIAEQWNITREEQDELALASQNKAEKAQAEGKFDEEIVPVVIKGRKGDTVVDKDEY
                     IKPGTTMEKLAKLRPAFKKDGTVTAGNASGINDGAAMLVVMAKEKAEELGIEPLATIV
                     SYGTAGVDPKIMGYGPVPATKKALEAANMTIEDIDLVEANEAFAAQSVAVIRDLNIDM
                     NKVNVNGGAIAIGHPIGCSGARILTTLLYEMKRRDAKTGLATLCIGGGMGTTLIVKR"
     gene            8049..8951
                     /locus_tag="ACOIAF_001912"
     CDS             8049..8951
                     /locus_tag="ACOIAF_001912"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HGZ2668923.1"
                     /translation="MFKKENQRYNLISYTNEEEMTLKEVLLDKLNFSVRSLSKMKREK
                     SVLVNGVYKKPSLKVYSGDLIEVKIDEEKANFEPQDLNLQIIYDDFDIIMVNKPPFMV
                     VHPTKSHYDKTIANGISYYIDNQKENVKIRFVNRLDMNTSGLVIVAKNAYAHHTLSTA
                     MSENKVEKKYITVVDGIIKENEGTIDEPIYRPTEDSIKRIIDERGQSSVTHYKVIERL
                     ENATVLEVSLETGRTHQIRVHMAHIGHGIIGDELYGYVDEELINRQALHAYKLEFEQP
                     RTKEKLKFKADIPEDMKELISKLR"
     gene            8965..9969
                     /locus_tag="ACOIAF_001913"
     CDS             8965..9969
                     /locus_tag="ACOIAF_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HGZ2668924.1"
                     /translation="MIIHKFIIHVLDKNSDVPILNDFEGKVNQEVDGFFQKAIKRIAK
                     DEDLRKGVFKDYNDNLIKNCCEQIIYDESTFLKNSKEIASYLFDVMKINAALESCDLA
                     ICLYTIKDEKSVAILKLDYKKLYTHSIEFVDDKFNIQMVSNEIGIPETLRQKQGALIS
                     LSGINDEFHLRLLDKDAEKEGSESKFVTEFLNAKKIDDDKYKTKVFKNTAENWITNAL
                     SNDIKQAEDVRSILNYTLKEKHEVDINDFVDNSIKDDELKDSFKEHMEEKGLDESFSI
                     DKKWVEKKLKKRSIKTDNGFDIKGNLTDFEDPMKYTVKQNQDGTIDIIIKNVTFYEEK
                     "
     gene            complement(10113..10340)
                     /gene="acpP"
                     /locus_tag="ACOIAF_001914"
     CDS             complement(10113..10340)
                     /gene="acpP"
                     /locus_tag="ACOIAF_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HGZ2668925.1"
                     /translation="MFEKIKEIIAEQLGVDNLDEITMEASLMDDLEADSLDAVEIIMA
                     LEDEFGIEIPDEEAENFKCIGDICKYIEENK"
     gene            10512..10937
                     /locus_tag="ACOIAF_001915"
     CDS             10512..10937
                     /locus_tag="ACOIAF_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668926.1"
                     /translation="MKYVGLLLTSVGMFLLIAVNFYYNSITLDMQRIEDYVMETNLIL
                     EDVAEKESYVSNEKEDYISRLMHVKKGIENSKTSFLIERYKEYKIKSIESLIYTISEE
                     KKDYLDEVDRYNKLGEKEINKLINKNFLEVTYLSITTYI"
     gene            10985..11191
                     /locus_tag="ACOIAF_001916"
     CDS             10985..11191
                     /locus_tag="ACOIAF_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668927.1"
                     /translation="MTKLEDNMERFFGGCEGFFGNKMLIIIVIVFLLLCTDILEDLLC
                     DDNIWIWVILIVLLLFNFDDGCCC"
     gene            11373..11552
                     /locus_tag="ACOIAF_001917"
     CDS             11373..11552
                     /locus_tag="ACOIAF_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668928.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFGDSWAEFDIMAWIPF
                     LIVLLILCCCGGCLE"
     gene            11586..11786
                     /locus_tag="ACOIAF_001918"
     CDS             11586..11786
                     /locus_tag="ACOIAF_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668929.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFEDCWRDIDIMSWIPF
                     LILLLITCSCGGFFDGEVGCGC"
     gene            12033..13055
                     /gene="ccpA"
                     /locus_tag="ACOIAF_001919"
     CDS             12033..13055
                     /gene="ccpA"
                     /locus_tag="ACOIAF_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator CcpA"
                     /protein_id="HGZ2668930.1"
                     /translation="MKMKGNITIKDVAKQAGVSISTVSRVINDSKPVTDEVKQKVLEV
                     IKETGYIPNPLARSLVTKKSQLIGVIVPEVSDSFVNEVLNGIEEVAKMYDYDILLANT
                     YSDKEQELKSINLLRAKQVEGIVMISWIVEQEHINYIQNCGIPATYISKTARNYDIYT
                     VSTSNEEATFDMTEHLIKKGHEKIAFIMTSKDDTVLEMERLAGYEKALSNNNIELDKS
                     LIKYGGTDYESGYNSMKELLDDGIIPHAAFVTGDEAAIGAINAICDAGYKVPEDISVA
                     GFNDVKIARMYRPKLTTVYQPLYDMGAVAIRMVIKLINKELIENKKIELPYRIVDRES
                     VTERKK"
     gene            complement(13178..13576)
                     /locus_tag="ACOIAF_001920"
     CDS             complement(13178..13576)
                     /locus_tag="ACOIAF_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668931.1"
                     /translation="MKKLDTESLEKMAKQKNIDKDKIEKMADSYKGKSENELMEELIK
                     IGKNLDGRDEVVSKFKAFLDENQRKKLDNIMEKISDAENQRDTKPAKTNKAKPTKGKS
                     NSPAPKNTPGSQQKKSKSLFKKTKKSNPHE"
     gene            13788..14699
                     /locus_tag="ACOIAF_001921"
     CDS             13788..14699
                     /locus_tag="ACOIAF_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668932.1"
                     /translation="MNNLLFLIGVIALSNGNISIFDDKNKSKGNNRVRKNKKNANDKL
                     SQSTHGNLRFDANDIKRGLRLMDLSEEDLEMGMEIITRTKKYMSRDERKILIKIESVL
                     DLVRGIKKLNNIDMVDTEDETDFFRKMDSDDKKNMMIKEIIDVFPEKRKNSVEKAIGM
                     KKKIDLFAELFLPDDFGEGGFSLSSLANINNLGSMNNLKLLGSLLRGDDSNNDDEDNN
                     DELNYEDEELDCEDKELDDDYDELTDEGEMEDNEDELVEDKNTGYENIENDEDIVYDE
                     ELYNINEQESEYNYNEKNTKTRAKKKR"
     gene            14814..14994
                     /gene="ssrS"
                     /locus_tag="ACOIAF_001922"
     ncRNA           14814..14994
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACOIAF_001922"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            15177..16394
                     /gene="cdeC"
                     /locus_tag="ACOIAF_001923"
     CDS             15177..16394
                     /gene="cdeC"
                     /locus_tag="ACOIAF_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeC"
                     /protein_id="HGZ2668933.1"
                     /translation="MQDYKKNKRRMMNQPMSTMNEEEVYTDEINSEDMRGFKKSHHHN
                     GCNTDNKCECHDDCNPCNPCNPCKPNPCNPCKPNPCDDNCGCHDNCKCDCEPCEMDSD
                     ECFENKCGPECCNPISPRNFSVSNAVPFAIEANRIFDTMQFQTFTDATGPNGEPLTFE
                     TEVVEVFGSVPSAGQASVTIEKICLSNDGIVIDTGMTTLEDFDLDPLGDIVGRNCETT
                     FEFAVCGERNAECCRQGKGKSVAYKQRGLTVAVRNLVLELRGRCGCTEFVALAFPAVR
                     AGGGCKRRVDYVEFTFNTLSAPICLPADGRAVTLRQEYQTNLTVDCIGKSILKLECNE
                     CCEPFYELIIPNDIDLVLCLQETVSTLISEQIVVLASPNPIQPRLVDTFSKVCDFSQC
                     GPNHGSGKPSCHR"
     gene            complement(16495..17817)
                     /locus_tag="ACOIAF_001924"
     CDS             complement(16495..17817)
                     /locus_tag="ACOIAF_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HGZ2668934.1"
                     /translation="MKVPYLVLSTVILFISNFIVRIFGFLYKIFLSRALGETGLGIYH
                     MIFNFLMICLAVTTTGIPTALSCLVAKRKALNDRHNTNALFISTLYISFFVALIISIV
                     ASFNSSFLSLKFLKDAKLNLFILAVCPAIVIITLSNVLRGYYYGIKNVKIPAIGQIIE
                     QIGKILFVFLLVMYINNKSMNCYIALLGISIGELSNIIFMLICLWRDSSFDNRYIISI
                     KDFYNSSMETLKMSIPITCNRMSNILLQSISSMMIPSRLALSGMTYQQSLSMYGIVSG
                     MVMPFIFLPFTVGSALIVNLIPTISQEMALRKRKSVIKKIKYSILLTLFVGILSSIFF
                     YFFGKDLCILVFKNTLAGEYLKAMFLVPLFMSLNQTLSGILHSIRKELASSINTITGM
                     LIQLIALYVFLPIPGLNIYAYIYTMTIVSIFTCLLHTIVLFKSLKSIR"
     gene            complement(17868..18662)
                     /locus_tag="ACOIAF_001925"
     CDS             complement(17868..18662)
                     /locus_tag="ACOIAF_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HGZ2668935.1"
                     /translation="MFYDYHMHSSFSTDGKSTMEEMVKKSIELGLEEICFTDHVDYDV
                     YADDSFSIVYEDYFKSLETLQSKYKDKISIKKGIEFGVQTQLMDTYKKEAHQYPLDFI
                     ICSIHAIDTMDLYLGNYFKDKTQHEVYENYYLYLYNIVKNYKDYSVLGHLDLIKRYAP
                     YDTILDDRLFSDIIEETLKQAIYDGKGIEINTSCYRYNLPDLTPSKYILQMYKDLGGE
                     IITTGSDSHHISQVACEFDYIYSLLKNMGFKYVSKFNKLKPEFIKL"
     gene            18772..20217
                     /locus_tag="ACOIAF_001926"
     CDS             18772..20217
                     /locus_tag="ACOIAF_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HGZ2668936.1"
                     /translation="MEKYRIDLNEKKSTKYIQIFNHIKELIINKELVEHEKLPPIRKL
                     SNFLQVNNTTIVKVYELLENEGYVYKIIGSGTFVSSLNSYKSGIDNRKNKIIVQREDL
                     IHFDNGNPSNDMFPIDSFKNAVNMALSKDGSSIFEYDEGLGSEELREKMVEYLSDGNI
                     KTTKENIQIISGAQQGIDIVCKGLISYSDVVFMEEPSYNGAIEVFKSRGAKIISIPML
                     DDGIDIGILKLKLEKIKPRLIYIMPNFQNPTGISYSTYKKKKLIELAEEHDFYIIEDD
                     FISDFVFESKDNRTIRSYDDKNRVVYIKSFSKILMPGLRIGIVEMPNELLKKVLWAKY
                     SSDISTPGLIQKSMYYYMNNFDWKNYLSHIEKIYTDKYKLAKNLINDKLSGKLKVRKS
                     CGGINFFLELPRGYTSQAFTNFMLNKGVSMLPGTYFFDNLVDDRFFRINIARPSMEEL
                     EKGISIISDNIDEFFCEYKNKLDIKSNKLFY"
     gene            20246..20455
                     /locus_tag="ACOIAF_001927"
     CDS             20246..20455
                     /locus_tag="ACOIAF_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HGZ2668937.1"
                     /translation="MFDKKKLDRINELAKKNKEGILSADEIKEREILRKEYLENFRAH
                     FRSRLDSVKVVSPEEYEQYMKNNKN"
     gene            20475..21869
                     /locus_tag="ACOIAF_001928"
     CDS             20475..21869
                     /locus_tag="ACOIAF_001928"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HGZ2668938.1"
                     /translation="MNLKYEFKNAWDFERKENTIEQIMQYSSNYMEFLSKSKTERLSV
                     KEIIKLAKENNYISIDEAMEKGSISCGDKIYVINKEKAVALFVIGKNYIEKGMKIIGS
                     HIDSPRLDLKPNPLYQESNLGFFKTHYYGGIKKYQWTAIPLALHGIVILNDGTKVDIS
                     IGEEDSDPVFCVTDLLIHLAGDQMQKKLSEGISGEALNILIGNMPLEDEEKEPITANI
                     LKILNEKYNIVEEDLLSAEIEVVPAGKARDLGLDRSMVLGYGHDDRVCSYAAVKAILE
                     TEQPEFTSVALCVDKEEIGSKGNTGMHSKFFENTVAELIALEGDYCDIKVRRALANSK
                     VLSADVSAGYDPNFGEAYEKRNSAYMGNGVVLTKYTGSRGKSGCNDANAEFMSEVKRI
                     FNKGNVVWQTAELGKVDQGGGGTIAHILANQGAEVIDCGVGVLNMHAPHEIVSKVDIY
                     EMYKGYKAFFNINL"
     gene            22109..22861
                     /locus_tag="ACOIAF_001929"
     CDS             22109..22861
                     /locus_tag="ACOIAF_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGZ2668939.1"
                     /translation="MDEKRQKKEMDLEVNSFVPLYQQLYDNIKKQIASGIYKPGDKLP
                     SEGDLCKEFNISRITVRNALNELVKEDILCKKRGKGTYVTIPERIEATCAGNSFTNSC
                     HRINAKPSTKIISVLIKKADKQVAEALSIELEEKVICIKRLRLIDEVPVIFEVDYFRI
                     DYMFLLKEELEDKSLMEVISNNISTLPKRVENIFEVKHSNKEYSDHLKCASNMPLLKV
                     RQSVYTENNDVLYYNEQFIRSDKYKYAVSAEI"
     gene            22887..23300
                     /locus_tag="ACOIAF_001930"
     CDS             22887..23300
                     /locus_tag="ACOIAF_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGZ2668940.1"
                     /translation="MKKIILASHGDFSKGLLNAVKMIVGDLADCVSSYGLYPGQSASD
                     FALELEKTISEDKECKYIILSDLYGASVCTAMLRLTNLHNVRLFSGMNLNMVLELLTR
                     FSDELTVEDIDQLVEESRRGIQSVTLQIKEEEEVF"
     gene            23304..24047
                     /locus_tag="ACOIAF_001931"
     CDS             23304..24047
                     /locus_tag="ACOIAF_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtpA/SgcQ family protein"
                     /protein_id="HGZ2668941.1"
                     /translation="MKKKGILSVFKSKKPIIAMIHLKGDTPEDIFERAKKEITIFEEN
                     GVDGIMLENYYGNYYDLERILEYVSKANLSIPYGVNCLNVDTMGFELATKYNASYIQV
                     DSVVGHVKPRDEATLEEFFKLQRSKCPAYLIGGVRFKYQPVLSENDVEEDLKIGMTRC
                     DAIAVTENATGQETSMEKIELFRKNLGDFPLVIAAGVTLENAKKQLKLGDMAIIGSYF
                     KDNYKDFGDVSVEHVKTFMDEIKKIREEL"
     gene            24047..24517
                     /locus_tag="ACOIAF_001932"
     CDS             24047..24517
                     /locus_tag="ACOIAF_001932"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGZ2668942.1"
                     /translation="MIKLVRVDHRLIHGQVAFTWTKFLSTDCILIASDDLLKDELRMA
                     GLKMAKPSNVKLVMKSIADSIKALNSGVTDKYNLLILCESVEDVYRLAKEVKAIKSIN
                     LGGTKSDDNRENISKAVHVSKDDIKMIKELDSEGVNVFVQLVPDDDATNVMKLI"
     gene            24534..25292
                     /locus_tag="ACOIAF_001933"
     CDS             24534..25292
                     /locus_tag="ACOIAF_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HGZ2668943.1"
                     /translation="MEFTQVILITLIAFFAYMHSFVGSTMHNRPIVVAPLVGLALGNL
                     HTGIVIGSTLELVFMGAFPVGASNPPDFVSGSIIATAFVILTGQDVSAAVVLAVPIAT
                     LVLLIDNFLMTVVLTWGAHIADRYAEEGNIEGVERVQLLFGIGNKLILAIIVGIGFSL
                     GVPVIEKILSFIPSYVTHGMDVAAGVIPAIGFAMLARMMLNKKTVAFLLLGFILVAYL
                     NITVTGVALFGLAIALIYVNFVGEKEVIVDDNEF"
     gene            25276..26118
                     /locus_tag="ACOIAF_001934"
     CDS             25276..26118
                     /locus_tag="ACOIAF_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HGZ2668944.1"
                     /translation="MTTSSKKLETISPDSKITRKDFWKCFRRSLTLDSSWNYERMQNI
                     AYAYMMAPIIRRLYKDDKEKKSKALKRHLEFMSVTPHISTLLVGISGAMEEENAKNKE
                     FDANSINAVKSSLMGPVSGIGDSFFWGTLKLIAAGVGIALASQGNIMGPILFLLIINV
                     PHFIIRYICLDKGFKYGTQFFKDVSGSSIVSKVMEAASMLGLMVIGGMTASNVMLKLS
                     VNVGSGEWAEPIQTYLDQIMPCMLPAMIFGIMYWLLGKKVKTTTILISVMIICIVLAA
                     IGVV"
     gene            complement(26278..27183)
                     /locus_tag="ACOIAF_001935"
     CDS             complement(26278..27183)
                     /locus_tag="ACOIAF_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism-associated LysR family
                     transcriptional regulator"
                     /protein_id="HGZ2668945.1"
                     /translation="MDFKQLEVFVAVAKHQSFSKAARELFLTQPTVSAHIQNLERELE
                     TVLINRSNKVITLTKSGEILYEHAIYILNNCKRAIYDIKEYSGKIEGIIDIACSSIPE
                     TYILPDFMKSFSMSYPDVKFSISHYDSQYAISEILNERISFGLVGSKINNPQIEYLDL
                     LDDELVLITPSDFKIDNKNNCIDIGELAYLNFIMRKEGSGTRNLILNTLSKNNFPVSK
                     LNVIAHVESNEAIKEMVRLGLGVSFISYISAIDYLNAGKIKCYKIKDVDFTRKFFFIY
                     SKKKTFSPLEDKFLNRLCEYFEIII"
BASE COUNT        10768 a         3019 c         4974 g         8653 t
ORIGIN      
        1 caatagcaca aaatataagg agactaaaaa tgaagaagtt tataaaagtt tcatttttta
       61 ttttggggat ttttacacta ttcatagcta taaataaatt agtatataga gatatggtaa
      121 gtgtcaaaga ttatgaaata aatcatttag aggaaattgt ttttgatttt gatagcagga
      181 gaagtagaga tgttggtact attgtaagat ttaaaataag caataatagt aaatatagtt
      241 ataaattaaa cagtgctaaa atgaagtttg aaaattgtgt ggaaaataaa aagggaatga
      301 ataagtgttc tgcatatgta aatgtagatt tatttaatga aaaaaattct ataactgaaa
      361 ggactctgaa atatggaata aaaccaaata atcaaggtta tgttgatttt gtaattccaa
      421 aaggactaaa ttttgattat aggtacttta atgaatctgg tatgagtgta gaatataaag
      481 gagaatatat tgtcaatatt ccaatggcaa aaggacttta tctagtagtt ggaaaaaata
      541 atgatacatg gaatgctaat attatggaat aatttgaaaa ataaatcaat tatagatata
      601 attaagaata atatgaaatt tataaaagtt cagactagga ggggtattta atgtcaataa
      661 gtaacgtaag agagtatttt aaacaatttg gcaaggaaga cagtatatta gaatttgaac
      721 aatctagtgc tactgtggaa cttgcagctg aagcagctgg ggttattcca gcaagaatag
      781 ctaagacact ctcatttaaa ataggagatg atgcaatttt aattgtgaca gctggtgatg
      841 ctaagattga taataagaag tataaggctg aatttaattg taaggctaaa atgttgacac
      901 cagaagaagt cttggagttt actggacatg ctattggtgg agtctgtcca tttggtttaa
      961 aaaattctat aaaagtctat ttagatgatt caatgaaaag atttgataca gtatttcctg
     1021 catgtggcag tagtaactct gcaatagaac ttacatgtga agaaatggag aagttttcta
     1081 agtgtgaaaa atgggtagat gtttgtaaga attggtaaaa aaatatagta aactatagta
     1141 ggaattagta aaaaactacc tctgtataca atctatacag agatagtttt tttgtgtaaa
     1201 tatttataaa tctaagagga aaatctaatt taaactaata atccaaaata aagttaaaaa
     1261 attctgattt tttttcgaaa caataaaaaa atgacaaaaa agtggaaaaa atgtaataaa
     1321 tattgttaaa aaaataatta gaaattattg aaaaattatc ctttgagtga tactattata
     1381 gatgtaagat atttaaaaat aaaaaatagt cagataaaaa tacgattatt tagctctatt
     1441 aaaataattt ttatacgata aaaatcttct gtacatggct aaaagacaga tatacagaca
     1501 atatttaatg tttaatatac cctaatataa atatcgtaaa caaattattt tatctgtaac
     1561 cagtgtgaaa gaaatcttat tctaacttga aatttcacaa aattccaaaa agcgagattt
     1621 tttttaagta tagttgattt taaattaaca atttatgatt aaattaatgt cataattggt
     1681 tagtataatc acatataaat atagagcgta gtttgattaa ggttataaat ttctcgacgt
     1741 atattcaaat accttattat agatgttgcc gagattttaa ttaataaatg aaaataaaat
     1801 atttataaaa tactgaggag gctattatgg atttaaattc taaaaaatat cagatgctta
     1861 aagagctata tgtaagcttc gctgaaaatg aagttaaacc tttagcaaca gaacttgatg
     1921 aagaagaaag atttccttat gaaacagtgg aaaaaatggc aaaagcagga atgatgggta
     1981 taccatatcc aaaagaatat ggtggagaag gtggagacac tgtaggatat ataatggcag
     2041 ttgaagaatt gtctagagtt tgtggtacta caggagttat attatcagct catacatctc
     2101 ttggctcatg gcctatatat caatatggta atgaagaaca aaaacaaaaa ttcttaagac
     2161 cactagcaag tggagaaaaa ttaggagcat ttggtcttac tgagcctaat gctggtacag
     2221 atgcgtctgg ccaacaaaca actgctgttt tagacgggga tgaatacata cttaatggct
     2281 caaaaatatt tataacaaat gcaatagctg gtgacatata tgtagtaatg gcaatgactg
     2341 ataaatctaa ggggaacaaa ggaatatcag catttatagt tgaaaaagga actcctgggt
     2401 ttagctttgg agttaaagaa aagaaaatgg gtataagagg ttcagctacg agtgaattaa
     2461 tatttgagga ttgcagaata cctaaagaaa atttacttgg aaaagaaggt caaggattta
     2521 agatagcaat gtctactctt gatggtggta gaattggtat agctgcacaa gctttaggtt
     2581 tagcacaagg tgctcttgat gaaactgtta aatatgtaaa agaaagagta caatttggta
     2641 gaccattatc aaaattccaa aatacacaat tccaattagc tgatatggaa gttaaggtac
     2701 aagcagctag acaccttgta tatcaagcag ctataaataa agacttagga aaaccttatg
     2761 gagtagaagc agcaatggca aaattatttg cagctgaaac agctatggaa gttactacaa
     2821 aagctgtaca acttcatgga ggatatggat acactcgtga ctatccagta gaaagaatga
     2881 tgagagatgc taagataact gaaatatatg aaggaactag tgaagttcaa agaatggtta
     2941 tttcaggaaa attattaaaa tagataaata ttaaaatata cacaataggt cgatattaaa
     3001 atagatatgg aggaaggatt tatgaatata gtcgtttgta taaaacaagt tccagataca
     3061 acagaagtta aactagatcc taatacaggt actttaatta gagatggagt accaagtata
     3121 ataaaccctg atgataaagc aggtttagaa gaagctataa aattaaaaga agaaatgggt
     3181 gctcatgtaa ctgttataac aatgggacct cctcaagcag atatggcttt aaaagaagct
     3241 ttagcaatgg gtgcagatag aggtatatta ttaacagata gagcatttgc aggtgctgat
     3301 acttgggcaa cttcatcagc attagcagga gcattaaaaa atatagattt tgatattata
     3361 atagctggaa gacaggcgat agatggagat actgcacaag ttggacctca aatagctgaa
     3421 catttaaatc ttccatcaat aacatatgct gaagaaataa aaactgaagg tgaatatgta
     3481 ttagtaaaaa gacaatttga agattgttgc catgacttaa aagttaaaat gccatgcctt
     3541 ataacaactc ttaaagatat gaacacacca agatacatga aagttggaag aatatatgat
     3601 gctttcgaaa atgatgtagt agaaacatgg actgtaaaag atatagaagt tgacccttct
     3661 aatttaggtc ttaaaggttc tccaactagt gtatttaaat catttacaaa atcagttaaa
     3721 ccagctggta caatatacaa tgaagatgcg aaaacatcag ctggaattat catagataaa
     3781 ttaaaagaga agtatatcat ataatttaag gggggataaa gaatgggtaa cgttttagta
     3841 gtaatagaac aaagagaaaa tgtaattcaa actgtttctt tagaattact aggaaaggct
     3901 acagaaatag caaaagatta tgatacaaaa gtttctgcat tacttttagg tagtaaggta
     3961 gaaggtttaa tagatacatt agcacactat ggtgcagatg aggtaatagt agtagatgat
     4021 gaagctttag cagtgtatac aactgaacca tatacaaaag cagcttatga agcaataaaa
     4081 gcagctgacc ctatagttgt attatttggt gcaacttcaa taggtagaga tttagcgcct
     4141 agagtttctg ctagaataca tacaggtctt actgctgact gtacaggtct tgcagtagct
     4201 gaagatacaa aattattatt aatgacaaga cctgcctttg gtggaaatat aatggcaaca
     4261 atagtttgta aagatttcag acctcaaatg tctacagtta gaccaggggt tatgaagaaa
     4321 aatgaacctg atgaaactaa agaagctgta attaaccgtt tcaaggtaga atttaatgat
     4381 gctgataaat tagttcaagt tgtacaagta ataaaagaag ctaaaaaaca agttaaaata
     4441 gaagatgcta agatattagt ttctgctgga cgtggaatgg gtggaaaaga aaacttagac
     4501 atactttatg aattagctga aattataggt ggagaagttt ctggttctcg tgccactata
     4561 gatgcaggtt ggttagataa agcaagacaa gttggtcaaa ctggtaaaac tgtaagacca
     4621 gacctttata tagcatgtgg tatatctgga gcaatacaac atatagctgg tatggaagat
     4681 gctgagttta tagttgctat aaataaaaat ccagaagctc caatatttaa atatgctgat
     4741 gttggtatag ttggagatgt tcataaagtg cttccagaac ttatcagtca gttaagtgtt
     4801 gcaaaagaaa aaggtgaagt tttagctaac taatttcttg gggagggtat tatgagtaca
     4861 agtgatgtta aagtttatga gaatgtagct gttgaagtag atggaaatat atgtacagtg
     4921 aaaatgaata gacctaaagc ccttaatgca ataaattcaa agactttaga agaactttat
     4981 gaagtatttg tagatattaa taatgatgaa actattgatg ttgtaatatt gacaggggaa
     5041 ggaaaggcat ttgtagctgg agcagatatt gcatacatga aagatttaga tgctgtagct
     5101 gctaaagatt ttagtatctt aggagcaaaa gcttttggag aaatagaaaa tagtaaaaaa
     5161 gtagtgatag ctgctgtaaa cggatttgct ttaggtggag gatgtgaact tgcaatggca
     5221 tgtgatataa gaattgcatc tgctaaagct aaatttggtc agccagaagt aactcttgga
     5281 ataactccag gatatggagg aactcaaagg cttacaagat tggttggaat ggcaaaagca
     5341 aaagaattaa tctttacagg tcaagttata aaagctgatg aagctgaaaa aatagggcta
     5401 gtaaatagag ttgttgagcc agacatttta atagaagaag ttgagaaatt agctaagata
     5461 atagctaaaa atgctcagct tgcagttaga tactctaaag aagcaataca acttggtgct
     5521 caaactgata taaatactgg aatagatata gaatctaatt tatttggtct ttgtttttca
     5581 actaaagacc aaaaagaagg aatgtcagct ttcgttgaaa agagagaagc taactttata
     5641 aaagggtaaa aatctaatta aaaatatatt tgctgttaac tagattaaat agtattttaa
     5701 tctagttaac agaatataaa tactaaaata acaggaggtt ataactatga aattagctgt
     5761 aataggtagt ggaactatgg gaagtggtat tgtacaaact tttgcaagtt gtggacatga
     5821 tgtatgttta aagagtagaa ctcaaggtgc tatagataaa tgtttagctt tattagataa
     5881 aaatttaact aagttagtta ctaagggaaa aatggatgaa gctacaaaag cagaaatatt
     5941 aagtcatgtt agttcaacta ctaactatga agatttaaaa gatatggatt taataataga
     6001 agcatctgta gaagacatga atataaagaa agatgttttc aagttactag atgaattatg
     6061 taaagaagat actatcttgg caacaaatac ttcgtcatta tctataacag aaatagcttc
     6121 ttctactaag agaccagata aagttatagg aatgcatttc tttaatccag ttcctatgat
     6181 gaaattagtt gaagttataa gtggtcagtt aacatcaaaa gttacttttg atacagtatt
     6241 tgaattatct aagagtatca ataaagtacc agtagatgta tctgaatctc ctggatttgt
     6301 agtaaataga atacttatac ctatgataaa tgaagctgtt ggtatatatg cagatggtgt
     6361 tgcaagtaaa gaagaaatag atgaagctat gaaattagga gcaaaccatc caatgggacc
     6421 actagcatta ggtgatttaa tcggattaga tgttgtttta gctataatga acgttttata
     6481 tactgagttt ggagatacta aatatagacc tcatccactt ttagctaaaa tggttagagc
     6541 taatcaatta ggaagaaaaa ctaagatagg attctatgat tataataaat aagaatttgg
     6601 gaattaaaag tttaaataaa attgtttaaa aaacaatttc ggatatatga aaaatctaat
     6661 ttaatggggg taatgaatat gagagaagta gtaattgcca gtgcagctag aacagcagta
     6721 ggaagttttg gaggagcatt taaatcagtt tcagcggtag agttaggggt aacagcagct
     6781 aaagaagcta taaaaagagc taacataact ccagatatga tagatgaatc tcttttaggg
     6841 ggagtactta cagcaggtct tggacaaaat atagcaagac aaatagcatt aggagcagga
     6901 ataccagtag aaaaaccagc tatgactata aatatagttt gtggttctgg attaagatct
     6961 gtttcaatgg catctcaact tatagcatta ggtgatgctg atataatgtt agttggtgga
     7021 gctgaaaaca tgagtatgtc tccttattta gtaccaagtg cgagatatgg tgcaagaatg
     7081 ggtgatgctg cttttgttga ttcaatgata aaagatggat tatcagacat atttaataac
     7141 tatcacatgg gtattactgc tgaaaacata gcagagcaat ggaatataac tagagaagaa
     7201 caagatgaat tagctcttgc aagtcaaaat aaagctgaaa aagctcaagc tgaaggaaaa
     7261 tttgatgaag aaatagttcc tgttgttata aaaggaagaa aaggtgacac tgtagtagat
     7321 aaagatgaat atattaagcc tggcactaca atggagaaac ttgctaagtt aagacctgca
     7381 tttaaaaaag atggaacagt tactgctggt aatgcatcag gaataaatga tggtgctgct
     7441 atgctagtag taatggctaa agaaaaagct gaagaactag gaatagagcc tcttgcaact
     7501 atagtttctt atggaacagc tggtgttgac cctaaaataa tgggatatgg accagttcca
     7561 gcaactaaaa aagctttaga agctgctaat atgactattg aagatataga tttagttgaa
     7621 gctaatgagg catttgctgc ccaatctgta gctgtaataa gagacttaaa tatagatatg
     7681 aataaagtta atgttaatgg tggagcaata gctataggac atccaatagg atgctcagga
     7741 gcaagaatac ttactacact tttatatgaa atgaagagaa gagatgctaa aactggtctt
     7801 gctacacttt gtataggtgg aggaatggga actactttaa tagttaagag atagttttag
     7861 attataatat ttacaaatta aatatttaat tatgaattta acctgtaaga ataactattt
     7921 gttattctta caggttttta tagttaaaaa taattattat ctattaaaca aaaatagtgt
     7981 aaaatatata atatcatgat tttgaagata taaaaagtag gaagatataa aagaaagtag
     8041 gtaaaagttt gtttaaaaaa gagaatcaaa gatataatct tatatcgtat acaaatgaag
     8101 aagaaatgac tttaaaagaa gttttattgg acaagctaaa tttctcagtt aggtcattat
     8161 caaaaatgaa gagggaaaag agtgttttag taaatggagt atataaaaaa ccgagtttaa
     8221 aggtgtatag tggtgattta atagaagtaa aaatagacga ggaaaaagca aattttgaac
     8281 ctcaagattt aaatttacag ataatttatg atgattttga tataattatg gtgaataaac
     8341 ctccgtttat ggtagttcac ccaacaaaaa gccactatga taaaacaata gcaaatggta
     8401 ttagttacta tatagataat caaaaagaaa atgtaaaaat aagatttgta aataggttag
     8461 atatgaatac ttcagggtta gttatagtag caaaaaatgc ttatgcacat catactttgt
     8521 ctacagcaat gagtgaaaat aaagttgaaa aaaagtatat aacagtagtt gatggaatta
     8581 taaaagaaaa tgagggaact atagatgagc caatttatag acctacagaa gattcaataa
     8641 agaggataat agatgaaaga ggacagtcat ctgttactca ctataaagta atagaaagac
     8701 tagaaaatgc aactgtatta gaagtaagct tagaaactgg tagaacacat caaataagag
     8761 tacatatggc tcatataggt catggaataa taggtgatga gttgtatggt tatgtagatg
     8821 aagagctaat aaataggcaa gcacttcatg catataagtt agagtttgaa caaccaagaa
     8881 caaaagaaaa attgaaattt aaagcagata taccagagga tatgaaagag ttaatatcga
     8941 aattaagata atggggtgat atatatgata atacataaat ttataataca tgttttggac
     9001 aaaaatagtg atgttccaat attaaatgac tttgaaggca aagttaatca agaagtagat
     9061 ggttttttcc agaaagctat aaaaagaatt gcaaaagatg aggatttaag aaaaggtgtt
     9121 tttaaagatt ataatgacaa tttaattaaa aactgttgtg aacaaataat atatgatgaa
     9181 agtacttttt tgaaaaattc taaagaaatt gcatcttact tgtttgatgt tatgaaaatt
     9241 aatgctgcac tagaatcttg tgatttggca atttgcttat acactattaa agatgaaaag
     9301 agtgtagcaa tactgaaatt agattataaa aagttgtaca ctcattcaat tgaatttgtg
     9361 gatgataaat ttaatattca aatggtttca aatgaaattg gtatcccaga gactttaaga
     9421 caaaagcaag gagctttgat tagtctaagt ggtataaatg atgagtttca tctaaggctt
     9481 ttagataagg atgctgaaaa agaagggtca gaatctaagt ttgtaacaga atttttaaat
     9541 gctaaaaaaa tagatgatga taagtacaag accaaggtat ttaaaaatac agctgaaaat
     9601 tggataacca atgctcttag taatgatata aagcaagctg aagatgtaag aagtatatta
     9661 aattatacac tgaaggaaaa acatgaagtt gatataaatg actttgttga taattcaatt
     9721 aaagatgatg agttaaagga tagctttaaa gagcatatgg aagaaaaagg tcttgatgaa
     9781 agttttagta tagataaaaa atgggttgag aaaaagctta aaaaaagaag tataaaaacg
     9841 gataatggtt ttgacataaa agggaatcta actgattttg aagacccaat gaagtatact
     9901 gtaaaacaaa atcaagatgg aactatagat ataataataa agaatgttac gttttatgaa
     9961 gaaaagtaat ttatatactt agtatataaa ttaaatataa gagcgtgtat tttatatatt
    10021 gtaaataaat gtaatctagt ctgtgcgaac tctaatataa tttattatgt agaagtttac
    10081 acagactatt tatatttgta gacaaaacga tactatttgt tttcttcgat atatttacat
    10141 atatcaccaa tacatttaaa gttctcagct tcctcgtctg gtatttctat tccaaattca
    10201 tcttcaaggg ccattataat ttcaacagca tctaatgaat cagcttctaa gtcgtccatt
    10261 aaagatgctt ccatagttat ctcatctaaa ttgtctaccc ctaattgttc tgctattatt
    10321 tcttttattt tctcaaacat tttaagcctc ctaaagcagt tttaattaat tttaatgtta
    10381 gtttaatata atatcaagaa tttttcaata tggaatctat aaaaaataat aaaattttat
    10441 aaatgtagta ataaagtatt atttgatgaa taaactttat tgataacaaa tataacttag
    10501 gaggaaatgc tatgaaatat gtaggactat tactaacttc agtaggaatg tttttactta
    10561 tagcagtaaa tttttattat aattcaataa ctttagatat gcaacgtata gaggattatg
    10621 taatggagac caatttaatc cttgaagatg tagctgaaaa ggaaagttat gtatctaatg
    10681 aaaaagaaga ctacatatca aggttaatgc atgtaaagaa aggaatagaa aattcaaaaa
    10741 catccttctt gatagaaaga tataaggagt acaaaataaa atcaatagaa agtttaatct
    10801 atactatatc agaggagaaa aaagattatc tggatgaagt tgatagatac aataaacttg
    10861 gtgaaaaaga aataaataaa ctaatcaata aaaacttttt agaagtaact tatctgtcta
    10921 ttactacata tatttaatta aagagacaaa acttagtctc ttaatgattc aaggaggatt
    10981 agttatgaca aaattagaag acaatatgga acgtttcttt ggaggatgcg aaggattttt
    11041 tgggaataaa atgttgataa taatagttat agtattctta ctattatgca cagacatttt
    11101 agaagactta ttatgcgatg acaatatatg gatatgggtt atattaatag tattgttatt
    11161 atttaacttc gatgatggtt gttgttgcta aaaaatattg attattaaat aggtaaaacc
    11221 agtaacttat acaagttatg gttttacctt ttttagttca aattaaaaaa tttaaaaaaa
    11281 tagtaaaaat acattagcaa aatggaaacc aaatgcatag tatatgttag gagcgaaaag
    11341 ctcaacaaaa tatcaacagg aggtaacgta taatgttaga tagattcttt ggagaagaag
    11401 gatgttttgg tggaggagga tggtggataa tagtactatt cttcctattc ttagcttttg
    11461 gagatagctg ggcagaattt gacataatgg catggatacc attcctaata gttttattaa
    11521 tactttgctg ctgtggtgga tgcttagaat aagaacacag ttattaaaaa aaggagggct
    11581 ttgttatgtt agatagattt tttggagaag aaggatgctt tggtggagga ggatggtgga
    11641 taatagtact attcttccta ttcttagctt ttgaagattg ctggagagac attgacataa
    11701 tgtcatggat acctttctta atattattgc taataacttg ttcatgtggt ggattctttg
    11761 atggtgaagt aggttgcggc tgctaatact taaatcatta gaataaaaaa atgctggtat
    11821 tttataccag cattttttta ttttattttt taatatgatt taaattaaga atgaatatta
    11881 ttaaatttgt tgaatatagg gtataatata aaatataatg tatttaaatg tacttgcttt
    11941 tatggtaaaa taaaaactta tataaatgta gttaaaatag gaaccttcaa aaatttggtt
    12001 cagttttaga taaatttagt aaaataagga ggatgaagat gaaaggcaat ataacgataa
    12061 aagatgttgc taaacaagca ggagtgtcaa tatctactgt atctagagtt ataaatgatt
    12121 caaaacctgt aactgatgaa gtcaaacaaa aagttttaga ggttataaaa gagactggat
    12181 atataccaaa tccacttgct agaagcttag taacaaagaa gagtcaatta ataggggtaa
    12241 tagttccaga agtttcagat tcttttgtta atgaggtgtt aaatgggata gaagaggttg
    12301 ctaaaatgta tgactatgat attcttttag cgaatacata ctctgataag gaacaagaac
    12361 ttaagagtat aaatctattg agagcaaaac aagtggaagg tatagttatg atttcatgga
    12421 tagttgaaca agaacatatc aactatatac aaaattgtgg aataccagcg acatatataa
    12481 gtaaaactgc tagaaattat gatatatata cagtaagtac tagcaacgaa gaagctactt
    12541 ttgatatgac agagcatctt ataaagaaag gtcatgaaaa gatagctttt ataatgacga
    12601 gtaaagatga tactgtttta gaaatggaaa gacttgctgg ttatgagaaa gcactttcaa
    12661 ataacaatat agaattagac aagagtttga ttaagtatgg tggaactgat tatgagagtg
    12721 gatacaatag tatgaaagaa ctattagatg atggaataat acctcatgcg gcttttgtaa
    12781 caggtgatga ggctgccata ggtgctataa atgctatatg tgatgctgga tataaggttc
    12841 cagaagacat atctgttgca ggatttaatg atgttaagat agctagaatg tatagaccta
    12901 aacttactac agtatatcaa cctctatacg atatgggagc agtagcaata agaatggtta
    12961 taaaattaat aaataaggaa ttaattgaga ataagaaaat agaattacct tatagaattg
    13021 ttgatagaga aagtgttaca gaaagaaaaa aataaaacta ttaaaatcaa tcaggtagtg
    13081 cctaaggcac tacctgattt tttatgtcaa taatatcaaa tttaataata gattaactaa
    13141 aaataattat ctaattactt aaatagttta tttttatcta ttcatgaggg ttagactttt
    13201 tagttttctt aaataaactt ttagattttt tttgttgact acctggtgta ttttttggtg
    13261 caggtgaatt tgacttacct ttagtaggtt tagccttatt agttttagct ggtttagtat
    13321 ctctttggtt ttctgcatct gagatttttt ccattatatt atccagtttt ttgcgttgat
    13381 tttcatctaa aaatgctttg aattttgata ctacttcatc tctgccatct agattcttac
    13441 ctatttttat aagttcctcc ataagttcat tttctgattt tcctttgtaa ctatcagcca
    13501 tcttttctat tttatccttg tcaatatttt tttgtttagc cattttttct aatgactctg
    13561 tatctaattt tttcataaaa aaaccccttt ctataaatat atattgaatt atattcatac
    13621 tatttatata tagaaataat attgaagtgg tgaattgtga gtgatatata atataaataa
    13681 aaattcttat agataatata tatgcactga gctttaaaaa tggaacatta aaatatgaaa
    13741 aacatacttt aatatagggc aattatataa tataaggggg aattgtcttg aataacttac
    13801 tatttttgat tggagtgatt gcattgagta atggtaatat ctctatattt gatgataaaa
    13861 ataaatcaaa aggaaataat agagttagaa aaaacaaaaa aaatgcaaat gataaacttt
    13921 cacagtctac acatggcaat ttaaggtttg atgcaaatga tataaaaaga ggtttaaggc
    13981 tcatggattt aagtgaagaa gatttagaaa tgggaatgga aataataaca agaactaaaa
    14041 aatacatgtc aagagatgaa agaaaaatac taataaaaat agaaagtgtt ttagatttag
    14101 ttagaggaat aaaaaaatta aataatatag atatggtaga tacagaagat gaaacggatt
    14161 ttttcagaaa aatggatagt gatgataaaa agaatatgat gataaaggaa attatagatg
    14221 tattcccaga aaaaagaaaa aattcagttg aaaaagctat aggaatgaaa aagaagatag
    14281 atttatttgc agaattattt ttaccagatg actttggtga aggtggattt agtttgagct
    14341 ctttggcaaa tataaacaat cttggtagta tgaataatct caaattgtta ggaagtcttt
    14401 taagaggtga tgatagtaat aatgatgatg aagacaataa tgatgagtta aattatgaag
    14461 acgaagaatt agattgtgaa gacaaagaat tggatgatga ttatgatgag ttaacagacg
    14521 aaggtgaaat ggaggataat gaagatgaat tagtagaaga taagaacaca ggctatgaaa
    14581 atatagaaaa tgatgaagat atagtatatg atgaggaact atacaacatt aatgagcaag
    14641 aatccgaata caattataat gaaaaaaata ctaaaactag agctaagaaa aaacgataat
    14701 taactaaata cataaagaaa aaaggaagaa aaagtaatga tagaataaga ttatctcaat
    14761 tactatataa aaccacttta aaaaattcaa aatttattat ataataataa tatgaaatcc
    14821 tgaaacatac gataaggctt ttttaaattc aacctacaaa tgaaatacgg gagaccgtgt
    14881 ctggtaaatt ataacatgcc tagttagttt ggacgttaaa ggttactggc aagtcaccca
    14941 cctgggagtg cctagggtgt gaatttactt agccaccggt gtttcgggac aaaaaaagtg
    15001 ctactatttc aaatagtagt acttttttta ttaatatgca tttttctact aaatacgcct
    15061 aattattgat aaattcaaaa agcaagtaac aattaagatt ataaaagcat agtattaatt
    15121 aggcttcaaa aagccaaaga cttaataaga atcgcttaca gggagggcaa tgaaaaatgc
    15181 aagattataa aaaaaataaa agaagaatga tgaatcagcc aatgtctaca atgaatgaag
    15241 aagaagtgta tacagatgaa ataaattcag aagacatgag aggttttaaa aaatcacacc
    15301 atcataatgg atgtaatact gataataagt gtgagtgcca tgatgattgc aatccatgca
    15361 acccatgtaa tccatgtaaa cctaacccat gcaatccatg caaacctaat ccatgtgatg
    15421 acaattgtgg atgccatgac aattgtaaat gtgattgtga accatgtgaa atggattcag
    15481 atgaatgttt tgaaaacaaa tgtggaccag aatgctgtaa tcctatatct ccaagaaact
    15541 tctctgtatc aaatgcagtg ccatttgcaa tagaggctaa tagaatattt gatactatgc
    15601 aattccaaac atttacagat gcaacaggac caaatggaga gccattaact tttgaaacag
    15661 aagtagtaga agtatttggt tcagttccaa gtgcaggtca agcaagtgta actatagaaa
    15721 aaatatgctt aagtaatgat ggaatcgtta tagacacagg aatgacaact ttagaagatt
    15781 tcgatttaga cccattagga gatatagtag gaagaaactg tgaaacaact tttgaatttg
    15841 cagtttgtgg agaaagaaac gctgagtgct gtagacaagg aaaaggcaaa tcagtagctt
    15901 ataaacaaag aggattaact gtagcagttc gtaatttagt actagagcta agaggtagat
    15961 gtggatgtac agagttcgtt gcattagctt tcccagcagt tagagcagga ggtggatgta
    16021 agagaagagt tgattatgta gaatttactt ttaacacact ttcagcacca atatgcttgc
    16081 cagctgacgg aagagctgtt actttaagac aagaatatca aactaactta actgtagatt
    16141 gtataggaaa atctatatta aaattagaat gcaacgaatg ttgtgaacct ttctatgaat
    16201 taattatacc aaatgatata gatttagtac tttgcttaca agaaacagtt agcacattaa
    16261 taagtgaaca aatagtagtt ttagcatcac caaatccaat ccaaccaaga cttgttgata
    16321 ctttctctaa agtatgtgat ttttcgcaat gtggacctaa tcatggaagt ggaaagccaa
    16381 gttgccacag atagataggt aagatatata gacagtttaa attataaata aaaatcatct
    16441 aaggaaggct accaaagtgt ataatttaca ttggtagcct tcacgttttt ataactagcg
    16501 tatagatttt agtgatttaa aaagcactat agtatgtaat agacaagtaa agattgatac
    16561 tatcgtcatt gtgtatatgt atgcataaat attaagtcct ggaattggaa gaaatacata
    16621 taaagctatt agttgaatta acatgccagt aatagtattt atacttgaag ctaattcttt
    16681 tcttattgaa tgtaaaatgc cagatagagt ttgatttagg gacataaata gtggaactaa
    16741 aaacatagct tttagatatt ctcctgcaag tgtattttta aatactaata tacataaatc
    16801 ttttccaaag aaataaaaaa atattgatga tagtatgcct acaaataaag taagaagtat
    16861 ggagtatttg atttttttaa ttacactctt acgctttctt agagccattt cctgtgaaat
    16921 agttggtatt aaatttacaa taagcgctga accaacagta aaaggtaaaa atataaaagg
    16981 cattaccatc ccactgacaa taccatacat acttaaagat tgttgataag tcattcctga
    17041 taatgctaat cttgatggta tcatcataga acttatagat tgcaaaagta tgtttgacat
    17101 tctattacaa gttatgggaa tagacatttt taatgtttcc atagaagaat tataaaaatc
    17161 ttttatgctt ataatatacc tattgtcaaa tgatgaatct cgccataagc aaataagcat
    17221 aaatataata ttactcaact caccaattga tatacctaat agagctatat aacaattcat
    17281 agatttatta tttatgtaca taacaagtaa aaaaacaaat aatattttac caatttgttc
    17341 aatgatttgt ccaatagcag ggatttttac attctttatg ccatagtaat atcctcttag
    17401 tacgtttgaa agagttatta taactatagc aggacaaaca gctagtataa acagatttaa
    17461 tttagcatct tttaaaaatt taagtgataa aaaagaacta ttgaaagatg caactataga
    17521 tataattagt gctacgaaaa atgatatata taaagttgat ataaataaag cattagtatt
    17581 gtgtctatcg tttaaagctt ttctctttgc aactagacaa ctaagtgcag taggtatacc
    17641 tgttgttgta acagctaagc aaatcatcaa aaagttaaaa atcatatgat agatacctaa
    17701 acctgtttca cctagtgctc ttgataagaa tattttatat aaaaaaccaa atattcttac
    17761 aataaaattg gatataaata aaataactgt tgatagtact aaataaggta ctttcaaaaa
    17821 aatcacctcc ttatctttta aatatattca caaggaggtg taaaaattta taattttata
    17881 aattctggtt tcaatttatt aaatttagat acatatttaa atcccatatt ttttagtaat
    17941 gaatatatgt agtcaaactc acatgcaact tgacttatat gatgtgaatc tgaacctgtg
    18001 gttatgattt cccctcccaa gtctttatac atttgaagta tatacttaga tggggttagg
    18061 tctggaagat tatatctata acaagaggta tttatctcga tacctttgcc atcataaata
    18121 gcttgtttaa gagtttcttc aattatatca gaaaatagtc tatcatctaa aatagtgtca
    18181 taaggtgcat accttttaat caaatctaaa tgacctaaga cagagtaatc tttataattc
    18241 ttaacaatat tatataaata aagatagtaa ttttcataaa cttcatgttg agttttatct
    18301 ttaaaataat taccaaggta taagtccatt gtatctatag catggataga acatattata
    18361 aagtccaatg gatattgatg tgcttccttt ttgtatgtat ccattaattg agtttgaact
    18421 ccaaattcaa ttcctttttt tattgatatt ttatctttat atttgctttg aagagtctct
    18481 aaactcttaa aataatcttc atagacaatt gaaaaagaat catcagcata tacatcatag
    18541 tcaacatggt ctgtgaaaca aatttcctcc aaaccgagtt caatagattt ttttaccatt
    18601 tcttccattg tagattttcc gtctgtagag aaacttgagt gcatgtgata gtcgtaaaac
    18661 ataaatattt ccccttttta taaaaatttt ggtattatat atacatacta aataatatca
    18721 aatatacata aaagcatcaa taaaatataa atacaatgag cggtgataaa tttggaaaaa
    18781 tacagaatag atttaaatga gaaaaaatca actaaatata tacagatatt taatcatata
    18841 aaagaattga taataaataa agagttagta gaacatgaaa aattgcctcc aataagaaaa
    18901 ctatcaaatt ttttacaagt aaataacact actatagtta aggtatacga gcttttagaa
    18961 aatgaaggat atgtatataa aattataggg agtggtactt ttgtatcaag tttaaattct
    19021 tataaaagcg gtatagataa caggaaaaat aagattattg tgcaaagaga agatttaatt
    19081 cattttgaca atgggaatcc atctaatgat atgtttccta tcgattcatt taaaaatgct
    19141 gtaaatatgg cattatctaa agatggtagt tcaatatttg aatatgatga aggtcttggc
    19201 tctgaagaat taagagaaaa aatggtagag tatctaagtg atggaaatat aaaaactact
    19261 aaggaaaata ttcaaataat atcaggtgct caacaaggta tagatatagt ttgtaaagga
    19321 cttataagtt attcagatgt tgtttttatg gaagaacctt cttacaatgg agcaattgaa
    19381 gtgtttaaaa gtagaggtgc aaaaataata tctataccta tgttagatga tggaattgat
    19441 ataggtattt taaaattaaa gttggaaaaa ataaaaccaa gacttatata cattatgcct
    19501 aattttcaaa atccaactgg aatatcttac tcaacatata aaaaaaagaa attgatagaa
    19561 ttggcagaag aacatgattt ttatataata gaagatgatt ttataagtga ttttgttttt
    19621 gaatcaaagg acaatagaac aattagaagt tatgatgata aaaatagagt agtatatata
    19681 aaaagctttt caaaaatact tatgccaggt cttaggatag gaattgtaga aatgccaaat
    19741 gagttgttaa aaaaagtttt atgggctaaa tattcatcag atatatcaac accaggttta
    19801 attcaaaaat caatgtatta ctatatgaat aattttgatt ggaagaatta tttgagtcat
    19861 atagagaaga tatatactga taagtataaa ttggcaaaaa acttgattaa tgacaaattg
    19921 agtggtaaat taaaagttag aaaatcttgt ggaggaataa actttttttt agaattacca
    19981 agaggatata catctcaagc atttacaaat tttatgttaa acaaaggagt gtcaatgctt
    20041 ccagggactt atttttttga caatctagtg gatgatagat tctttaggat aaatatagct
    20101 cgcccaagca tggaagaact agaaaaggga atatctataa taagtgataa tatagatgag
    20161 tttttttgtg aatataaaaa caagttagat ataaaaagca ataaattatt ttattaaata
    20221 aatagaggtt ttttaggagg aaaaaatgtt tgacaagaag aaattagata ggattaatga
    20281 attagcaaag aaaaataaag agggaatcct ttctgctgat gaaatcaaag aaagagaaat
    20341 tttaaggaaa gaatatttag aaaactttag agcccacttt agatcaagac tagatagtgt
    20401 caaagtagtt tcaccagaag aatatgagca atacatgaaa aacaataaaa attagaactt
    20461 agtaggagga aaatatgaac ttaaagtacg aatttaagaa tgcttgggat tttgaaagaa
    20521 aagaaaatac tattgaacaa attatgcaat attctagcaa ttatatggag tttttaagta
    20581 agtcaaagac tgaaagactt tcagtaaaag aaattataaa attggctaaa gaaaataact
    20641 atatttcaat agatgaagct atggaaaaag gaagcataag ttgtggtgat aagatttatg
    20701 ttataaataa agagaaggca gtagcacttt ttgtaatagg taaaaattat atagaaaaag
    20761 gtatgaagat tataggaagc catatagatt cgccaagatt agatttaaaa ccaaatccac
    20821 tgtatcaaga gagtaatcta ggttttttta agactcatta ttatggtgga ataaaaaaat
    20881 accaatggac ggctattcca cttgctttac atggtatagt tatattaaat gatggaacaa
    20941 aagtggacat atctattggg gaagaagata gtgacccagt attttgtgta actgatttat
    21001 tgattcattt ggctggagac caaatgcaaa agaaactatc agaaggaatt agtggagaag
    21061 ctttaaatat attaattggg aatatgcctt tagaagatga agaaaaagaa cctataacag
    21121 caaatatatt aaagatttta aatgagaaat acaatatagt tgaagaagat ttattaagtg
    21181 ctgaaattga agtagttcca gctggtaagg ctagagattt agggcttgat aggtctatgg
    21241 tgttgggata tgggcatgat gatagggtat gttcatatgc tgctgtaaaa gctatactag
    21301 aaactgaaca accagaattt acttcagttg cattatgtgt agataaagaa gaaattggtt
    21361 ctaaagggaa tacaggtatg cactcaaagt tttttgaaaa tactgtggct gaactgattg
    21421 ctttagaagg agattattgt gatattaaag taagaagagc tttagcaaat agtaaggtat
    21481 tatcagctga tgtttctgct ggatatgacc caaattttgg agaagcatat gaaaagagaa
    21541 attcggcata tatgggaaat ggagtagttt taactaagta tacaggttca agaggaaaga
    21601 gtggatgtaa tgatgctaat gctgaattta tgtcagaagt aaagagaatt tttaataaag
    21661 gaaatgtagt atggcaaact gctgaacttg gaaaagtaga ccaaggagga ggcggaacta
    21721 tagcccatat attggcaaat caaggtgcag aggttataga ctgtggtgtt ggagttttaa
    21781 atatgcatgc accacatgag attgtctcta aagtagacat atatgaaatg tataagggat
    21841 ataaggcatt ttttaatata aatttataag taaaaatata aatgtaataa gtgaataaag
    21901 gctgttgtat aaacaattta atctgttgta caacagtctt atttttatgt aaaaatagca
    21961 atttgtcaca ctagagaaaa taaaagaaaa tactgtcaaa ttaaaaatta tttaaaaaaa
    22021 aattgacaac gatttcatct tgttataata tgtaataaag aacttgttat ataatgttat
    22081 aaaaatgtta catagggagg tgagcaaaat ggatgaaaag agacaaaaaa aagaaatgga
    22141 tttagaagta aattcctttg ttcctttata tcaacaacta tatgacaaca taaaaaaaca
    22201 aatagcatct ggcatatata aaccaggaga taaacttcca tctgaaggag acttatgtaa
    22261 agaatttaat ataagtcgta taactgtgag aaatgcactt aatgaacttg taaaagaaga
    22321 tatattatgt aaaaaacgtg gtaaagggac ttatgtaaca ataccagaga gaatagaagc
    22381 aacatgtgca ggaaatagtt ttactaattc ttgtcatcgt attaatgcaa aaccaagtac
    22441 caaaataatc tctgttttaa taaaaaaagc agataagcaa gtagctgaag ctttaagtat
    22501 tgaactagaa gagaaggtaa tatgtattaa aagacttaga ttaatagatg aagtacctgt
    22561 tatttttgaa gttgactatt ttagaataga ttacatgttt ttattaaaag aagaattaga
    22621 agataaatct ttaatggagg ttatatctaa taatataagc acattaccta aacgagtaga
    22681 aaatatattt gaagtaaaac attcaaacaa agaatattct gaccatctta aatgtgcaag
    22741 taatatgcca ctattgaaag taagacaatc ggtgtacaca gaaaataatg atgtcttata
    22801 ttacaatgag caatttataa gaagtgataa gtacaaatat gcagtatcag cagaaatata
    22861 atctataatt tagtaggagg atgagtatga aaaagataat attagcatct catggagact
    22921 tttcaaaagg gcttttaaat gcagtaaaaa tgatagtagg agatttagca gattgtgtca
    22981 gttcgtatgg tttgtaccct ggacaaagtg cttcagattt tgctttagaa ttagaaaaaa
    23041 caatatcaga agataaagaa tgtaaatata taattttatc tgacttatat ggagcaagtg
    23101 tatgtacagc aatgttaaga ttaactaatc tacataatgt taggttattt tctggaatga
    23161 atttgaatat ggtattagaa cttcttacaa ggttttctga tgaattaaca gttgaagata
    23221 tagaccaatt agtggaagaa tctagaagag gaatacaaag cgtgacatta caaattaaag
    23281 aagaagaaga ggtattttag tttatgaaaa aaaagggaat attatcagta tttaaaagta
    23341 aaaaaccaat tatagcaatg atacacttaa aaggagatac tccagaagat atctttgaaa
    23401 gagctaaaaa agagattaca atctttgaag aaaatggtgt agatggaata atgttagaaa
    23461 attactatgg taattattat gatttagaaa gaatacttga atatgtatca aaagctaatt
    23521 tatcaatacc atatggagta aactgtttaa atgttgatac tatgggattt gaactggcaa
    23581 caaaatataa tgctagttat atacaggttg attctgtagt aggtcatgtt aaacctagag
    23641 atgaagctac tttagaagag ttttttaaat tacaacgttc aaaatgccca gcgtatttaa
    23701 ttggaggcgt acgttttaaa tatcagccag tattatcaga aaatgatgtt gaagaagatt
    23761 taaaaatagg aatgacaaga tgtgatgcta tagcagttac agaaaatgct acaggtcaag
    23821 aaacatcaat ggaaaaaata gaactgtttc gtaaaaattt aggagacttc ccattggtta
    23881 tagctgctgg agttacattg gaaaatgcaa aaaaacagct taaattagga gatatggcaa
    23941 taataggaag ttactttaaa gataattaca aagattttgg ggatgttagt gtagaacacg
    24001 taaaaacatt catggatgaa atcaaaaaaa ttagggagga attataatga ttaaattagt
    24061 aagagtagac cacagactta tacatggaca agtagcattt acatggacaa agtttttaag
    24121 tacagactgt atattgatag caagtgatga cttattaaaa gatgaattga gaatggcagg
    24181 acttaaaatg gctaaaccat ctaatgttaa gttagtaatg aaaagcatag cagattctat
    24241 aaaagcactt aactcaggtg ttactgataa atacaactta ttgatacttt gtgaatctgt
    24301 agaagatgtt tatagacttg ctaaagaggt aaaagctatt aaatctataa accttggtgg
    24361 aacaaaatca gatgataatc gtgaaaatat atctaaagca gtgcatgtat caaaagatga
    24421 tataaagatg attaaagagt tagattcaga aggcgtaaat gtatttgtac aattagttcc
    24481 tgatgatgat gctacaaatg taatgaaatt aatataattt tagggggatg aaaatggaat
    24541 ttacacaagt tattttgata actctaattg cattctttgc atatatgcat agttttgtag
    24601 gttctacaat gcataataga cctatagttg ttgcaccact tgttggttta gcactaggta
    24661 atcttcatac aggtatagta ataggttcaa cattagaatt agtatttatg ggtgcatttc
    24721 ctgttggggc aagtaatcct cctgattttg tgtctggttc tattattgcc acagcgtttg
    24781 taatattaac gggtcaagat gtatcagcag cagttgtact tgcagttcct atagcaactc
    24841 tagtattgct tatagataac ttcttaatga cagtagtact tacatgggga gctcatattg
    24901 ctgaccgtta tgcagaagag gggaatattg aaggcgtaga acgtgtgcaa ttattatttg
    24961 gtataggaaa taaattaata ttagcaataa tagtaggtat aggattttca ttaggtgttc
    25021 cagtaataga aaaaatactt tcatttatac catcatatgt aactcatgga atggatgttg
    25081 cagcaggcgt gattccagca ataggttttg ccatgttagc tagaatgatg cttaataaga
    25141 aaacagtagc atttttatta ttaggattta tattggttgc ttacctaaac ataacagtaa
    25201 caggagttgc attgtttgga ttagcaatag ctttaatata tgttaacttt gtaggagaga
    25261 aggaggtaat agtagatgac aacgagttct aagaaattag agacaatttc accagatagt
    25321 aaaataacga ggaaagactt ttggaaatgt tttagaaggt cattgacatt agattcatct
    25381 tggaactacg aacgtatgca aaatatagca tatgcataca tgatggcacc aataatccgt
    25441 agattatata aagatgataa agaaaagaaa tctaaggctt taaaaagaca tttagaattt
    25501 atgtcagtta ctcctcatat aagtactctt ttggtaggta tatctggagc tatggaagaa
    25561 gaaaatgcaa agaataaaga atttgatgct aatagtataa atgctgttaa atcaagttta
    25621 atgggtccag tttctgggat aggagattca tttttctggg gaacattaaa gcttatagca
    25681 gctggggttg gtatagcttt agcttctcag ggtaacataa tgggaccgat tttattcctt
    25741 ttaataataa atgttcctca ttttattata cgctatatat gtctagataa aggatttaag
    25801 tatggaactc aattctttaa agatgtgagt ggttcaagta tagtttcaaa agttatggaa
    25861 gcagcttcaa tgttagggct tatggtaatt ggaggtatga ctgcatctaa tgtaatgtta
    25921 aaacttagtg ttaatgtagg tagtggagaa tgggcagagc ctattcaaac ttacttagac
    25981 caaataatgc catgtatgct tccagctatg atattcggta ttatgtattg gttattagga
    26041 aaaaaggtaa agacaactac gatattaatt tcagttatga ttatttgtat agtattggct
    26101 gctattggag tagtataaat aaaaaaatcc caagttcatt tgaacgaggg attttttgac
    26161 taaattaaat aaaaaagaaa gatggttcat aataatttat aagcatttat gtgatagtta
    26221 taattagctt attaattagt tataaactca aaattaagca ctactttatt aaagttatta
    26281 tattataatt tcgaaatact cacataatct gtttagaaat ttatcttcta atggggaaaa
    26341 agttttcttt ttagagtaaa tgaagaaaaa ctttctagta aaatcaacat cctttatttt
    26401 ataacatttt attttacctg cattgagata atcgatagct gaaatgtacg atataaagga
    26461 tactccaaga cctaatctaa ccatttcctt aatagcctca tttgattcta catgagcgat
    26521 tacatttaat ttacttactg gaaagttatt tttactaagt gtgtttagta ttaagtttct
    26581 tgtacctgac ccttcttttc tcataataaa atttagataa gccaattctc ctatatcgat
    26641 acaattattt ttattatcaa ttttaaaatc tgaaggagtg ataagaacaa gttcatcatc
    26701 taataagtct aggtactcta tttgaggatt attgatttta gaaccaacta atccaaagct
    26761 tattcgttca tttaaaattt ctgatatagc atattgagaa tcataatggc ttatggaaaa
    26821 ttttacatct ggataactca tagaaaaact cttcataaaa tcaggtaaaa tataagtttc
    26881 aggtatggaa ctacatgcta tatctatgat accttctatt tttcctgaat attcttttat
    26941 atcataaatt gcacgtttgc aattattcaa tatatatata gcatgttcat aaagaatttc
    27001 accagatttt gtgagtgtaa taactttgtt gcttctattt ataagaacag tttctagctc
    27061 tctctctaaa ttctgtatgt gtgcgcttac agtaggttga gttaaaaaaa gttctcttgc
    27121 agcctttgaa aaactctgat gttttgcaac agcaacaaat acttcaagtt gtttaaaatc
    27181 cattatttaa tcctccttaa attatctaaa actattataa catatttgat aaattaatag
    27241 tcaaaggtga tttttaaaat agaaaacctt taaatattca tggagttttt gattgtttag
    27301 gaataatttg gtataatgtt gtaaggtact tacaataaaa atatcgtaaa ttgaattttc
    27361 aaaattcaac tttaaagtgt tttttattac agaaaaatta caattagctt ctta
//
LOCUS       DBGJFF010000046        27348 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.046, whole genome shotgun sequence.
ACCESSION   DBGJFF010000046 DBGJFF010000000
VERSION     DBGJFF010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27348
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.046"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            269..802
                     /gene="pyrR"
                     /locus_tag="ACOIAF_001936"
     CDS             269..802
                     /gene="pyrR"
                     /locus_tag="ACOIAF_001936"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HGZ2668946.1"
                     /translation="MVEKAQLMDEKAIARAITRISHEIIERNKGVENLVLVGIKTRGV
                     PIANRISKKIEQIEGTKVDTGDIDITLYRDDLEKIHVEPVVKGTYLDFNVNDKTVVLV
                     DDVLYTGRTVRASLDAIIDIGRPKSIQLAVLVDRGHRELPIRADYVGKNVPTSRHEII
                     SVSLLEIDGEDSVTIKE"
     gene            1086..2297
                     /locus_tag="ACOIAF_001937"
     CDS             1086..2297
                     /locus_tag="ACOIAF_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HGZ2668947.1"
                     /translation="MKKTIMSLQHLLAMFGATVLVPILTGFNPSVAIFCAGVGTLIFH
                     FCTEGKVPAFLGSSFAFIPVILAAKEAYGGDLAYAQGGIIVAGLIYIIMSIIVKVVGV
                     NKIKLYFPAQVTGAMIVVIGLNLLPTAFSMASANFVIAFITLAIAILTNKFGRGFIKQ
                     LGILIAVFSGYIICLILRLVDITTITEASLFAIPNFTVPKFSLGAIVIISPVVLAVFM
                     EHIGDMTTNGAVVGKNFIENPGLNRTLLGDGFATIVAGCLGGPANTTYGENTAVLAIT
                     KNYDPSILRRTAIFAILLACVGKFGGFLQSIPGSVMGGISIMLFSMITYVGLKTIRDS
                     SCVESKINILIIAVILLIGLGTTYLSNKGISVGIPITSTVKITGLSLAAIVGIVLNRI
                     LNNQDFKVEEE"
     gene            2596..4248
                     /locus_tag="ACOIAF_001938"
     CDS             2596..4248
                     /locus_tag="ACOIAF_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein"
                     /protein_id="HGZ2668948.1"
                     /translation="MLLVKVENLKKYYADKLILDIDKLEILENDKIGLVGSNGQGKTT
                     LLKAILGEIEIDEGYTYLTESYSYISQSENNIETCGHSKEKSLLNAPDKFEEHLSGGE
                     KVKLKIADALSNKKNIIIADEPTSNLDKKSIGVLEDMFKRHEGALLLISHDRRFLDEL
                     CTTILELEDGKLKAYKGNYTDYLMQKDEEVKRADFEYQEYVKEKKRLEKALLYKKALS
                     DGIRKTPKRMGNSEARLHKMGGQTNKKKLDSNVKAIKSRIDKLEVKNKPKVSKEMNIK
                     MQDGMEIISKNLVEVKDMTLKLENKLLLDNVSFKIKRGKKIALLGDNGCGKSTLIKEI
                     LADKNDNIKINNKVKVGYFDQNQSLLDEEKSVLYNTKVNSSFDESFIRINLSLFGFKG
                     DDVYKKVKVLSGGEKVKIALCKIILEDNNFLVFDEPTNYLDIKSMEALEKALINTDKT
                     MLIVSHDRVFVSHICNYIIEIKDAKIREFDCNYDEYTISRNKKTPSRENQIKKENLLV
                     LENRLTTVISMLSIEKDNLKKELYESEYNELLKQINKLKNSF"
     gene            complement(4463..6238)
                     /locus_tag="ACOIAF_001939"
     CDS             complement(4463..6238)
                     /locus_tag="ACOIAF_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HGZ2668949.1"
                     /translation="MALDGLVIHSIVDELSSKLTGGKIDKIHQPEDDEVIFNIRNNKE
                     NFRLVLSASASNPRVYLTSNYQKENPLKAPMFCMLLRKYIQGGNIVEISQIGFERIIK
                     ISVESLDELKEKTVKNIMIEIMGRHSNIIITHGEENKIIDSIKRVPFSISRVRQVLPG
                     HDYSLPPEQNKLNPLDDISKDLFIKNLEELEGPIFKSIYSRFLGISPIIAKEICYRAG
                     VNQNAIIKDISDEQFDSLHKVFCNLFNDINSNKYSPCIIIDKKVDKVVDFSCINLTLF
                     SDLSYINKDSMSRILEDFYRTKDIKDRINQRSSDLKKSISVKLDRLYNKLKKQEEELS
                     ESENADIYKIKGELITSYIYMVEKGMESIEVANFYDENCNDVTIELNKNLTPSENAQK
                     YFKKYNKMKHAKVEISHQISLNKEEIDYLENIILSIENCENLAELQDIKEELAKVGYI
                     KTQKKNSKKDTIPSTKPHEFVSSDGFKILVGKNNKQNDYLTLRLADNDDLWMHTKNIP
                     GSHVIIKCAGKEVPDNTVFEGAMLAAFFSKSKLSSQVPVDYTKRKNVKKPSGSKPGMV
                     IYETNSTIYVTPEEETVAKLKVKSE"
     gene            6534..7568
                     /gene="ltaE"
                     /locus_tag="ACOIAF_001940"
     CDS             6534..7568
                     /gene="ltaE"
                     /locus_tag="ACOIAF_001940"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HGZ2668950.1"
                     /translation="MRFIDLRSDTVTMPTDEMREAMAKAPVGDSIFRDDPTVNKLEEL
                     AAAKVGKEDAIFLPSGTFGNQLALFTHCLRGQEIIIGKGYHIVTHEVGAPAVIAGVQL
                     RTIDEDESGALNPELVEKAIRRDDIHEPQTGLICVENAYSGGTVVSLDNLREIKKIAE
                     KHNLPVHLDGARLFNAALNLGVEAKEIAKCCDSVMFCVSKGLAAPMGSILAGSKEFIA
                     KARRKQKVMGGGMRQVGIVAAAGIIAIEKMTLRLNEDHENAKYLACELNKIDGIEVNN
                     VNPDISMVFFKMSEDIIKEDVLIKEFFERNIKINKMENGEYRFVTHVDITKEDLDYVL
                     KTLKELIGVC"
     gene            7855..8685
                     /gene="dapF"
                     /locus_tag="ACOIAF_001941"
     CDS             7855..8685
                     /gene="dapF"
                     /locus_tag="ACOIAF_001941"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HGZ2668951.1"
                     /translation="MKFWKLHGIGNDFIAIDGRFDQINPSDYTALAKKVCHRRFSVGA
                     DGLLVVKDSKVADVEMVYYNSDGSRAAMCGNGLRCFCKFVYDNCIVKKESFTVDTLDG
                     IKDIKVNLTNREINSIRVNMGKGSFIAKDVPVLTNKERFVQESIKVLDRELKVTSMLM
                     GVPHTVVIVDELDVDDVCKYGKEIENNKIFPEKTNVNFVKVEDKYNIHVYTWERGCGY
                     TLGCGTGMTASAIVCNLLNMVESSVNVTSQGGTVKIDVGDFSYMTGPAVKICEGVLEV
                     "
     gene            8689..9570
                     /locus_tag="ACOIAF_001942"
     CDS             8689..9570
                     /locus_tag="ACOIAF_001942"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HGZ2668952.1"
                     /translation="MAISMTGFGRGEYKDDNYYFLVECKTINHKYSDINIRLPRKISF
                     LEDKVRNLVKNYVKRGRVDLYIKFDLLGKEDVNLNFDEGLASQYIDILKEIKNKFDII
                     DDISVMNVAKFPDIVKIEEKEEDEDLLWSMLNQAVEDALIKLREMRSEEGKKLAEDIA
                     MRCDLLKNHIEEIEKYSSSVVEDYREKLNLRISELLDDPSIIDENRLAQEVAIYADKS
                     SITEEIVRFKSHIGQLKNTIFKDDSIGRKIDFLIQEMNRETNTIGSKSSDINITNLVV
                     EVKSELEKIREQIQNIE"
     gene            9600..10217
                     /gene="gmk"
                     /locus_tag="ACOIAF_001943"
     CDS             9600..10217
                     /gene="gmk"
                     /locus_tag="ACOIAF_001943"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HGZ2668953.1"
                     /translation="MFVKKGLLLVVSGPSGAGKGTICKELLKENDTIKLSVSATTRKP
                     RTGEVDGVNYFFISKEKFEEMIEKGEFLEYAQIYDNFYGTPKAAIMECLEKGQDVLLE
                     IEMQGAKQIKEVCPEGVFIFVLPPSLKELKNRIVGRGTETEAEIEKRFSCAYEEIKMI
                     KDYDYFIFNEDVKTSAKEIEGIISSEKNKVSRYKNIIIEKFKEEL"
     gene            10220..10486
                     /gene="rpoZ"
                     /locus_tag="ACOIAF_001944"
     CDS             10220..10486
                     /gene="rpoZ"
                     /locus_tag="ACOIAF_001944"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HGZ2668954.1"
                     /translation="MLKPSINEVLEKIDNRYYLVGTVSKRARKLIDGEEPYVSNKTKE
                     KPVCVATKEVASGKITYRLLTEEEIEIEEARHHAEQHQQISEEE"
     gene            10491..11690
                     /gene="coaBC"
                     /locus_tag="ACOIAF_001945"
     CDS             10491..11690
                     /gene="coaBC"
                     /locus_tag="ACOIAF_001945"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HGZ2668955.1"
                     /translation="MLKDKTVVIGISGGIAAYKACDVVSKLKKLNANIHVIMTKSATE
                     FVKPLTFQSLSQNYVVEDMFEEPKTWEVEHISLAKKADLFLIVPASANVIGKIANGIA
                     DDMLTTTVMATKAQVLIAPAMNTNMYENPIVQKNIQTLKSFGYKFIEPESGRLACGDI
                     GTGKLANPDIIVEEVLTNLTKEQDLKGQSMIVTAGPTVESIDPVRYLTNRSSGKMGYS
                     IAREAIARGADVTLISGPTNLSIPQNLKKFVQIESAQDLYEAVVSNLSENKIVIQSAA
                     VADYKPKTYSDKKIKKKNDDLSIELSRNYDIAYEIGKIKEDKILIGFAAETNDLIEHA
                     KGKIKKKNLDFIVANDLTKEGAGFRADTNIVKIIDVEGNITEYPKMKKEEVANVILDK
                     VKELLEK"
     gene            11965..14454
                     /gene="priA"
                     /locus_tag="ACOIAF_001946"
     CDS             11965..14454
                     /gene="priA"
                     /locus_tag="ACOIAF_001946"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HGZ2668956.1"
                     /translation="MKKYAKVIVRSNTIYTDNLFTYQIPVFLSDVIKIGHRILVPFGK
                     GNKPTEAFVFQFTDSLDEKIKIKEIIDVLDENPIFRKEDLELVYWMKNRYLCTYIECI
                     NLIYPKGYKLNNYKVVLLGESLSGLNDIELKEKISTLSDKNREIVEKVIDSKGKIKVD
                     KLKYIPNLNSSLYTMNKNGIIKLCWEYKNHKNEKKVCYISLSLESDKIDDYIEQNKIN
                     VGSKQKEILSFLKNNENVEINDLLDLLNASKQSINSLSKKKLITLEFKDYYREPKSIY
                     KSVLKSIKLNNEQQEAVDEIKSNMFVDDKKPYLIHGVTGSGKTEVYMEIIEFALNQGL
                     DSIFLVPEIALTPQTIDRLKSRFGDLVGVFHSKLSEGEKHDVYKAVKAGKVRVLIGAR
                     SALFAPFNSLGLIIIDECHESSYKSEKNPKFNAIEVARFMALKNNITLILGSATPSIE
                     EYYRAKSGEYKLINIKSRANDKPLPNIEVVDMKDELDKGNRSIFSLKLQKEIRYAIEE
                     NNQVILFLNRRGYANFVSCRKCGYVFQCENCDISLTYHKKSNTGRCHYCGYEKEIPKE
                     CPECKSTYVKPFGVGTQKIEEELKYIFPDIKTLRMDKDTTSKKGALDEILNKFKDKEA
                     DVLIGTQMLSKGLDFENVTLVGILSADMILNFPDFKSFETTFQLITQVSGRAGRADKE
                     GKVVLQTYDTEHYAIKHAIEYDYEGFYEDEIKIRKAFGYSPFNNMLSVVVSGEDERLV
                     IKNIKNMHASLIYLLEKRGINDLGFILGPNPCSISKINQNYRWQILFKDENIEINLLK
                     GIIKYICITKRDLIFDKNINVSIDINPNSVL"
     gene            14472..14912
                     /gene="def"
                     /locus_tag="ACOIAF_001947"
     CDS             14472..14912
                     /gene="def"
                     /locus_tag="ACOIAF_001947"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HGZ2668957.1"
                     /translation="MALRQIVQIGEPVLRKKSKKVEKIDEKIIQLLDDMAETMYDADG
                     VGLAAPQVGILKRVVVIDIGEELIELINPEIIETSGEQIDEEGCLSVVGEAGNVRRPN
                     YVKVRALNRNGETIELEGEELLARAFCHEIDHLDGILFVDKIEK"
     gene            14927..15856
                     /gene="fmt"
                     /locus_tag="ACOIAF_001948"
     CDS             14927..15856
                     /gene="fmt"
                     /locus_tag="ACOIAF_001948"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HGZ2668958.1"
                     /translation="MKIVFMGTPDIAVPCLQKIIDEKYEILGVVTQPDKPKGRGKKLG
                     MSPVKELAIENNIPVYQPVKARDKEFIDKIKSLNPDVIVVVAFGQILPKEILEIPKLG
                     CINVHVSLLPKYRGAAPINWVIINGEEKTGVTTMYMDEGLDTGDMILKTEVNLDENIT
                     AGELHDKMMNIGAETLKETLRLIEEGNAPREVQNHEEFSYAPIMNKSLGNIDFSKSAR
                     EIHNLVRGVNPWPSAYTTYNDVIMKVWKTKVLDEKSTKDVGTIIDVSKDGIKVSTIDN
                     VLLIEEIQMPNKKRMLVGEYIKGNTIETGLVLG"
     gene            15866..16618
                     /locus_tag="ACOIAF_001949"
     CDS             15866..16618
                     /locus_tag="ACOIAF_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HGZ2668959.1"
                     /translation="MTDIKKYLTSVGILILILGILIGVVNFFIISLTQIFSLMINIII
                     ISLILIILCSTAVTYRVIKGKYVNSNIMKINFNIVSGLFPIISFIASSFGMSKSDIRR
                     IYIKLNNEYIYSNKYNFNPEDIIILIPHCIQENSCKLKVTNDIDNCKECGRCNIGELI
                     KLKEKTNVKIFVATGGTLARKIIMDTKPKAVVAVACERDLTSGIQDIKKIPVLGVFNK
                     RPNGPCVNTNVDMMDIEKAIGFLTGKNILVCN"
     gene            16687..17388
                     /locus_tag="ACOIAF_001950"
     CDS             16687..17388
                     /locus_tag="ACOIAF_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HGZ2668960.1"
                     /translation="MYPVYGGFWGFDPTMVVLIPAILLTIYAQFKVSSTTNKYLRVNT
                     RRGYTGEQTARRVLDSNGLYDVKIEMVRGHLSDHYDPRRKAVRLSEDVYYGTSITSVA
                     VAAHECGHAIQHAKGYAPLQIRSSLVPVVNFASSISWFLIFLGFIMAGPFLKIGILLF
                     SASVLFQIITLPVEFNASSRAIVQLGNLGIIDESESRQSRRVLSAAALTYVAAALVSI
                     LQLLRLLLIAQRRND"
     gene            17450..18775
                     /gene="rsmB"
                     /locus_tag="ACOIAF_001951"
     CDS             17450..18775
                     /gene="rsmB"
                     /locus_tag="ACOIAF_001951"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HGZ2668961.1"
                     /translation="MDAREVGFKVLCDIEKNDNYSNIAINKHFKNLEISDMDRGLATE
                     LIYGVIENKYYLDYIINKLSKIKVKKMSTYVKIFLRMGTYQILFLDSISDYAAVNETV
                     KLSKKYDKKSSGFINAILRNEIRAKETIMDITEEDSVKYLSIKYSYNSWIIKNWIDKF
                     GQEFAEDLLEANNEKPSIYIRTNTLKISREELIEKLSEMGIVCLKVPMVEEAIKVEKM
                     KNIEKNELFKAGLFTIQDISSMIVGKVINPKEGSFVLDVCSAPGGKSTHLATLMNNTG
                     QVVARDIFEHKLKLINSTVNRLGLKNVFVERFDASEIDENSISKFDYVLADVPCSGFG
                     IIRRKPEIKYKKEEELKDITSIQKKILENASKYVKVGGTLVYSTCTIQDVENINIVTS
                     FVEENHNFEFVPIDTVNVDLDNQDKGYLKIYPNIHGIDGFFIAKLKRIR"
     gene            18783..19814
                     /gene="rlmN"
                     /locus_tag="ACOIAF_001952"
     CDS             18783..19814
                     /gene="rlmN"
                     /locus_tag="ACOIAF_001952"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HGZ2668962.1"
                     /translation="MEKKKIVLKNFTEDELKEFMKTIDEKPFRGSQIFSWIYKGAKTF
                     DDMNNIPKSLRNKLEEVSCIGHIDIELKLESKVDNTKKYLFLLDDGNIIETVMMDYDS
                     RVTVCVSNQVGCRMGCNFCASTMDGLIRNLEPWEILDQVIKIQEDTGKRVSNLVLMGS
                     GEPLDNFENTKQFLKIINEKNGLNIGYRHITLSTCGIVPKMYELADLEIAINLALSLH
                     SPYDEERRKIMPVANAYSIEEILNACRYYIKKTNRRVTFEYSLIKGVNDSEKEAKALA
                     KLLKGMLCHVNLIPINKVEEREYEKPDKAFIYKFRDSLEKNNIPATVRMSMGSDISGA
                     CGQLRRKYK"
     gene            19834..20586
                     /locus_tag="ACOIAF_001953"
     CDS             19834..20586
                     /locus_tag="ACOIAF_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HGZ2668963.1"
                     /translation="MVYSCASHIGKIRKNNEDYCEGEVIDTEHGPIGIFAIADGMGGH
                     KKGEVASKLAVENIIDFLKENLLQHDNVKIDYIDDILKQAYNNVNSIVHKKSMEDIEF
                     EGMGTTLVTAIVYNNVLYVANVGDSRCYLLTDEKFDKITIDHSVVEELMMAHVITEEE
                     ARRHPQRNRITRAIGTDDMVVVDIFKKEIKKSDIILLATDGLTGFIDDEDIRDLILDY
                     EYERTSNISEELISMANDVSGKDNVSVIVIKV"
     gene            20603..22618
                     /gene="prkC"
                     /locus_tag="ACOIAF_001954"
     CDS             20603..22618
                     /gene="prkC"
                     /locus_tag="ACOIAF_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861606.1"
                     /note="PrkC, as found in Clostridium difficile, has a
                     kinase domain, two PASTA domains, and a C-terminal
                     Gly-rich repeat region; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing Ser/Thr kinase PrkC"
                     /protein_id="HGZ2668964.1"
                     /translation="MGDTILGNRYEIIRKIGDGGMAFVYEAKDRLLNRTVALKVLRPE
                     FVDDDEFLTKFKREAEAVASLSHPNIVNVYDVGEDGKVHYIVMEFVDGKNLKEIIQDE
                     GILDEYTALDITKQIAMALSAAHKKGIIHRDIKPHNILISNEGRVVKVADFGIAKAVS
                     NSTMTNIGSIIGSVHYFSPEQAKGKFVTNNADLYSLGIVLYEMLIGKVPFRGDSPISI
                     ALQHINDDIDFTSEEKVRIPQSVRTTIKKLTEKSSADRYQTAEELIEDIEYIEKNIDL
                     DFIKEYDDFATKKIDEKEINKVVNPTLAKPAPEKVVKPVEVADLDDDEDYYDDFYEED
                     DDEDEEEEEIMRAKKNQRPKSTPSKRTKKKKKKQESPKSRRRLKVIAAVLILILCAQV
                     FLAYKFLFAGGFGNKSLTVPNLVNMTLEEAQSAVEKEGLYLSVKSEEYNSEVDENCII
                     SQTPEGGSTNVKKGDTINVVVSKGSSQASVPNVVGLTLSNAKQLIEENNLKVGTVKYE
                     YSSIYKEGTVLSQSPGAGSSRAQEGDEVNLYVSKGSEKSNTQTPTVPDKKPTTPENNT
                     PTEPGSNSGNSGGNSGGSNSGNSGGNSGNNGSNSGGSNSGNSGGNSGGSNSGDNGGSS
                     GGSNSGDNGGSSGGSNSGDNGDSSGGSNSGDNGGTSSGTGANIGKTE"
     gene            22726..23463
                     /locus_tag="ACOIAF_001955"
     CDS             22726..23463
                     /locus_tag="ACOIAF_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGZ2668965.1"
                     /translation="MKKKTVLITGGARGIGKAMSKAFAKEGYNVLVNFNKSENEAKEL
                     YTILNEKNFSVKLFKADISNREDVEDMVDYCIKEFGGLDVLVNNAGVSQDKLFTDITD
                     EDWDNMMNINLKGSFYCSQVALKYMISEKKGNIINISSIWGISGASCEVHYSITKAGI
                     IGMTKALAKEVGPSNIRVNSIAPGVINTDMLSGYNEEDIDALVEETPLMRLGTPEDIA
                     NCAIFLASDKSNFITGQVISPNGGFVI"
     gene            23561..24445
                     /gene="rsgA"
                     /locus_tag="ACOIAF_001956"
     CDS             23561..24445
                     /gene="rsgA"
                     /locus_tag="ACOIAF_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HGZ2668966.1"
                     /translation="MLEGKIIKGISGFYYVDTYNGIYECKARGIFRKQKITPLVGDRV
                     KISIVDEDEKKGILEEIDSRDTELIRPPIANVDKALIVFAIKNPKPNLSLLDRFIVLA
                     EKENLETVIILTKADLDDNDTLETVKNIYELSGYKVIPVSNITKLNIDKVKEELKENV
                     VVFAGPSGVGKSSLLNEIDENFKLQTGVVSDKIKRGKHTTRHAELLKLEFGGMVADTP
                     GFSSLVLEDIEEVELKDYFIEFDKFNDCKFGSKCIHENEPNCAIKEAVTNGEISKERY
                     DSYIQLLHEIRQNNSRRY"
     gene            24450..25121
                     /gene="rpe"
                     /locus_tag="ACOIAF_001957"
     CDS             24450..25121
                     /gene="rpe"
                     /locus_tag="ACOIAF_001957"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HGZ2668967.1"
                     /translation="MIKLAPSILSADFAKLLEDVRKVESAGCEYLHIDVMDGHFVPNI
                     TLGPLVVKSLKKENINMVFDAHLMIENPDQYIEEFVKAGCDIITVHQEACVHLHRTIQ
                     NIKSHGIKAGVVLNPATPVDTIKHVLPDLDMVLLMSVNPGFGGQSFIPCVLDKIKELK
                     AIIDSQGLNIDIEVDGGISPKNVAEVVQAGANVIVAGSAIFGSDDIQETVNLFRKNAS
                     LEELV"
     gene            25130..25771
                     /locus_tag="ACOIAF_001958"
     CDS             25130..25771
                     /locus_tag="ACOIAF_001958"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HGZ2668968.1"
                     /translation="MKICIVLNGEIEDYEVTRDIIIKECYDCIICADGGANHTYKMEI
                     MPDYILGDLDSVEEEKISFYKNKGVKFEKFPSKKDETDTELCLFLAKTLKANHIDFFG
                     ALGGRIDHTLANIKLLYYLKEDGIYSRILSDKEEMYIVENEEISLYGNPGDTISVIAI
                     NGDAKGVTLTGLEYPLDNYYMKYSVPIGISNVMLSNSCKIKVEQGCVLVVRNL"
     gene            25902..26531
                     /locus_tag="ACOIAF_001959"
     CDS             25902..26531
                     /locus_tag="ACOIAF_001959"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HGZ2668969.1"
                     /translation="MVDSIIFDLDGTLWDSTEEVCKVWQDVLSEHKEIELSVTKDLFR
                     SLMGLSFEEIANRLFPNLSEEERMNLLNECSLKECDYLSENGAKLFDDIEDTLKLLSK
                     KYKLFIVSNCQAGYIESFLKAHKLEQYFIDFECPGNTGLHKGENNKLIIERNKLINPI
                     YVGDTQGDANSAKFVGIPFIYAKYGFGNVDVYDYFIDSFKDLLEHDILK"
     gene            26586..27242
                     /locus_tag="ACOIAF_001960"
     CDS             26586..27242
                     /locus_tag="ACOIAF_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HGZ2668970.1"
                     /translation="MELQDTIFKRQSVRKFKNQDVSDEDILKMIKAAGAAPSGKNIQN
                     WHFVVIKRRDLIEKIADVITKKQQEILVEMDKVSVDKANRFRKFVKNFTLFYLKAPVL
                     VLVFTKVYNPSGYYELELIDAPKETIDKLFIRNPGMQSLGAAIENFTLSAIELGYGSC
                     WLTSQNYAADEIEAVLEAETGFEKGEYFLGAMLALGVPEDNLKSPSKKPVEEICTFIK
                     "
BASE COUNT        10885 a         2764 c         5103 g         8596 t
ORIGIN      
        1 tttgtttaaa aaagcttgaa aaatttgaaa tataaatata taatttgatt tgatagatta
       61 aaaaattatc tttaaacgat acagagatat cgtaagacaa ttgctaaatt gcactgtata
      121 ttttttatat aagagcttct tgtcttgtga tgagaagttt ttttgttgtt taaattgaaa
      181 ttttaaataa aaataaattt ataaaaaatt aaaagaaagg agtagatttg tcaaatagat
      241 atattatatc tttgacaata attatgatat ggtagaaaaa gctcaattaa tggatgaaaa
      301 ggcaatagca agagcaatta ctagaataag tcatgaaata attgaaagaa ataaaggtgt
      361 agaaaatcta gttttagttg gaataaagac tagaggtgtg ccaattgcaa atagaatttc
      421 taagaaaata gaacaaatag aaggaacaaa agtagataca ggagatattg atataacttt
      481 gtatagagat gatttggaaa aaattcatgt tgaacctgta gttaaaggaa cttatttaga
      541 ttttaatgta aatgataaga cagtagtttt agttgatgat gttttataca ctggaagaac
      601 tgtgagagct tcactagatg ctattataga tataggaaga cctaaatcaa tacaattagc
      661 agtgctagtg gacagagggc atagagagtt gcctataaga gcagattatg taggaaaaaa
      721 cgtacctact tctagacatg aaattatatc tgtcagttta ttagaaattg atggagaaga
      781 ttcagttaca atcaaagaat aattattttt aagtattata taaaagctta gcattgaata
      841 gtcagggagg aggataaagc ttacatagat tgcacaaaat aaatattata attttatgga
      901 tgtgagtctt aatgtagttt ttattctcct ttatttgagt ttttgatgtc gtaaaatgta
      961 aaataatgat aaattatata ataagtatat agaggttaaa aaatagaatc tagggaatga
     1021 taattaatta aaagatatta attaaacaga ggatttattt atatataata attttggagg
     1081 tttttatgaa aaaaacgata atgtctttac aacatctatt agccatgttt ggagcgacag
     1141 tattagttcc cattttgaca ggttttaatc cgtcagttgc aattttctgt gctggtgttg
     1201 gtacattgat atttcatttt tgtacagaag gaaaagtacc tgccttttta ggttcaagtt
     1261 ttgcgtttat accagttata ctagcagcta aagaggcata tggtggagat ttagcatatg
     1321 ctcaaggtgg tataatagta gcaggattaa tttatattat aatgtcgatt atagtaaaag
     1381 tagttggtgt aaataaaatt aaattatatt ttcctgcaca agttacagga gctatgatag
     1441 tagttatagg attaaattta ttgccaacag cttttagtat ggcttcagcc aattttgtga
     1501 tagcatttat aactttagca atagcaattt taaccaataa gtttggtaga ggatttataa
     1561 aacaattagg aatattaata gcagtttttt ctgggtatat aatctgtttg atattaagat
     1621 tagtagatat aactactatt acagaagcaa gtttatttgc aatacctaac tttacagttc
     1681 ctaagttcag tttaggagca attgtgatta tatctccagt tgtattagct gtatttatgg
     1741 aacatatagg agatatgact actaatggtg ctgtagtagg taaaaacttt atagaaaatc
     1801 ccggattaaa tagaacatta cttggggatg gatttgcgac tatagttgca gggtgtttag
     1861 gaggaccagc aaatacaact tatggagaaa atacagcagt acttgctata actaaaaact
     1921 atgacccttc aatacttaga cgtacagcaa tatttgctat attgcttgca tgtgtaggca
     1981 agtttggtgg atttcttcaa agtataccag gttcagttat gggtggaatt agtataatgc
     2041 tattttcgat gataacttat gtaggattaa aaactataag ggacagttct tgtgtagaaa
     2101 gtaaaataaa tatattaatc atagcagtta tattattaat aggtcttgga acaacatatt
     2161 tatctaataa aggtatttct gttggaatcc ctataacaag tacagtaaag ataactggtc
     2221 ttagtttagc tgcaattgtt ggaattgtat taaacagaat tttaaataat caggacttta
     2281 aagttgaaga agaataaatt taaagaataa gttgtatatt gaataactaa aattagttaa
     2341 ttttctatag acatttgtaa ttaatgttga tataatgatt aaaaataaat gaaagaaagg
     2401 gcaaggtaac taggacaatg agaatataat ctatttttta taatttaaag tattgtaatt
     2461 tttgaaaagc ctattttggt ggctttattt gcaatgctta aattaaggag agtagattat
     2521 agtctatacg tttatttgtt atgcctattt atagaatctc tccttatatt taagtgtgtt
     2581 aaggagggat tttttatgtt gttagtaaaa gtagagaatt taaagaaata ttatgcagat
     2641 aaattaattt tggatataga taaacttgaa atattagaaa atgacaaaat aggtctagtt
     2701 ggttcaaatg gtcaaggaaa aacaacatta ttaaaagcaa tattgggaga aatagaaatt
     2761 gatgaaggat atacttacct tacagaaagc tattcttata taagtcagag tgaaaataat
     2821 attgaaacat gtggtcatag taaggagaag agtcttttaa atgctccaga taaatttgaa
     2881 gaacacttat caggagggga aaaggttaag ttaaaaatag cagatgcgct gagtaataag
     2941 aaaaatatca taatagctga tgagccaact tcaaacttag ataaaaaaag tattggggtt
     3001 ttggaagata tgtttaaaag gcatgaggga gcattattat taatatctca tgatagacgt
     3061 tttctggatg agttatgtac aactatatta gaactagaag atggaaaact aaaagcttac
     3121 aaaggcaatt acactgatta tttaatgcaa aaagatgaag aagtaaaaag agctgatttt
     3181 gagtatcaag aatatgttaa agagaaaaaa aggcttgaaa aagctctttt atataaaaaa
     3241 gctttaagtg atggcataag aaaaacacca aaaagaatgg gtaattcaga agctaggttg
     3301 cacaaaatgg gtggtcagac taataagaaa aagttggact caaatgtgaa ggctataaaa
     3361 agcagaattg ataaacttga ggtaaagaat aaacctaaag tttctaaaga aatgaacatt
     3421 aagatgcaag atggtatgga gataattagc aaaaatctag tagaggtaaa agatatgact
     3481 ctaaagttag aaaataagct cctgttagat aatgtttcct ttaagataaa aagaggtaaa
     3541 aagatagcat tattaggtga taatgggtgt ggaaaaagta ctctgataaa agaaatattg
     3601 gctgataaaa atgacaatat aaaaataaat aacaaggtaa aagtaggtta ttttgaccaa
     3661 aatcaaagtt tattagatga ggaaaagagt gttctataca atactaaagt taatagttca
     3721 tttgatgaat cttttataag gataaactta agcctatttg ggtttaaagg tgatgatgtc
     3781 tacaaaaaag taaaagtact tagtggtgga gaaaaggtaa aaatagcact atgtaaaata
     3841 atattggaag ataataattt tttagtattt gatgaaccaa caaattactt agatataaaa
     3901 tctatggaag cactggaaaa ggcactgata aatactgata aaactatgct tatagtgtct
     3961 catgatagag tatttgtatc acatatttgt aactatatta tagagataaa agatgctaaa
     4021 ataagggagt ttgattgtaa ttatgacgaa tatacaatta gcagaaataa aaaaactcct
     4081 agtagagaaa accaaatcaa aaaagaaaat cttctagtat tagaaaatag acttacaact
     4141 gttatatcta tgttatctat agaaaaagat aatctaaaaa aagaattgta tgaatcagaa
     4201 tataatgaat tactaaaaca aatcaataaa ttaaaaaata gcttttagtg ttaacttttt
     4261 aattacatct atataagaag actgaaaata tttgttggga atataagtat agttattaaa
     4321 acagaaaagt attttgttag aaaattaaaa gtaagctaat tagaaagtgg ccttattatt
     4381 gtactttata agaaggccac tatttttatt tagaatgata atgtgtagtt gtctaacatt
     4441 tattttgtat ttagtgcaga atttattcag atttaacctt aagcttggct actgtttctt
     4501 cttcaggagt aacataaata gtactgttag tttcataaat aaccatacct ggtttagaac
     4561 cactaggttt tttcacattt tttctcttag tatagtctac aggcacctga gaagaaagtt
     4621 ttgacttact aaagaatgct gctagcatag ctccttcaaa tacagtatta tcagggactt
     4681 ccttaccagc acactttatt attacatgag aaccaggaat attctttgta tgcatccata
     4741 agtcatcatt atcagcaagt cttagtgtaa gatagtcatt ttgcttattg tttttaccaa
     4801 ccaatatttt aaatccatca gaagacacaa attcatgagg ttttgtagat gggatagtat
     4861 cctttttact gtttttcttt tgagtcttga tgtatccaac tttagcaagt tcttctttta
     4921 tatcttgtaa ttcagctaaa ttttcacagt tttcaatact taaaattatg ttttctaagt
     4981 aatctatttc ttccttattt aaggaaattt gatgagatat ctcgacttta gcatgtttca
     5041 ttttgttata ctttttaaag tatttttgag cattttcaga tggtgtcaag tttttattta
     5101 attctattgt tacatcatta caattttcat catagaagtt agcaacttca atactttcca
     5161 tacctttttc taccatataa atatatgaag taatcaattc tccttttatc ttataaatgt
     5221 ctgcattttc ggattctgat aattcttctt cttgtttttt aagtttatta taaagtctat
     5281 caagcttaac agatatactc ttctttaaat cagatgaacg ttgattaatt ctatctttaa
     5341 tatccttagt tctatagaaa tcttccagaa tacgagacat actatcctta tttatatatg
     5401 ataaatcgct aaataatgta aggtttatac aactgaaatc aactacctta tccacctttt
     5461 tatctataat aatgcaagga gagtatttgt tagaatttat atcattgaat aaattacaaa
     5521 atactttgtg taaagaatca aattgttcat cagatatatc ctttatgata gcattttgat
     5581 ttacacctgc tctgtagcaa atttctttag ctataatagg gcttatacct agaaatctag
     5641 aatatataga cttaaagata ggtccttcca attcctctag gtttttaata aacaaatcct
     5701 tagatatatc atcaagaggg ttgagtttgt tttgttctgg tggtaatgaa taatcatgac
     5761 caggtaaaac ttgacgaact ctacttatgc taaaagggac tctttttata gaatctataa
     5821 ttttattttc ttcaccatga gttattatta tgttgctatg tcttcccata atttcaatca
     5881 taatattttt aactgtcttt tcttttaatt catctaaaga ttcaacactt atttttatta
     5941 ttctttcaaa acctatttga gaaatttcta ctatatttcc accttgtatg tattttctaa
     6001 gtaacataca aaacataggc gcttttagag ggttttcttt ttgataatta cttgtaagat
     6061 aaactctagg atttgaggca cttgcactta aaaccaatct aaaattttct ttattatttc
     6121 ttatattaaa aattacctca tcatcttcag gttgatgtat cttatctatt tttccacctg
     6181 taagttttga tgataattca tcgactattg aatgtataac taatccatct aatgccattt
     6241 atagcacccc tttctttgac caaaatgagt cagtaaatta attatacaca taatatacgc
     6301 tttgtgacta gggggaattt aaacaataga gattaatatt taaattgtat gattatttat
     6361 atttattaga gaggaaactt tatttttaaa attaataatt taaagtagat taaatataaa
     6421 gtaaatatag tataattatt gtgtagagta taagctatga aagtatatga actttacaga
     6481 aaaaatcaat tttaaggttc tatagtatat aaatatgaaa gaaggttgaa aaaatgagat
     6541 ttatcgattt aagaagtgat actgttacta tgcctactga cgaaatgaga gaagctatgg
     6601 caaaggctcc tgttggagat tcaatcttta gagatgaccc tactgtaaat aaacttgaag
     6661 aattagcagc agctaaagtt ggaaaagaag atgctatttt ccttccaagt ggaacttttg
     6721 ggaatcaatt agctctattt actcattgcc taagagggca agaaataatt attggtaaag
     6781 gttatcacat tgtgactcat gaggttgggg cacctgctgt aatagctgga gttcagctta
     6841 gaactataga tgaagacgaa agtggagctc taaatcctga acttgttgaa aaggcaatta
     6901 gaagagatga catacatgaa ccacagacag ggcttatatg tgtagaaaat gcatattcag
     6961 gtggaacagt tgtgagtctt gataatttaa gagaaattaa aaaaatagct gagaaacata
     7021 acttaccagt acatttagat ggagcaagat tatttaacgc agcattaaat ttgggtgtag
     7081 aagctaaaga aatagcaaag tgttgtgata gtgttatgtt ctgtgtgtca aaaggtttag
     7141 ctgcaccaat gggttctata cttgctggaa gtaaagaatt tatagcaaaa gctagaagaa
     7201 agcaaaaagt tatgggtggg ggaatgagac aagtaggtat agttgcagca gctggtataa
     7261 ttgccattga aaaaatgact ttaagattga atgaagacca tgaaaatgca aaatatttag
     7321 cttgtgaatt aaataaaatt gatggcattg aagtaaataa tgttaatcca gatataagta
     7381 tggtattttt taagatgagt gaagatatta taaaagagga tgttttaata aaagaatttt
     7441 ttgaaagaaa tataaaaatt aataaaatgg aaaatggtga atatagattt gtaactcatg
     7501 tagatattac taaggaagat ttagactatg ttttaaaaac acttaaagaa ttaataggtg
     7561 tatgttaata gatatctact agaaggatta tagctagtta ccagatgttt aattaaaaaa
     7621 ttcatagaca gtttaaataa aacatacata aaattcatat agaaatatta atatataaac
     7681 atagaaagaa ctagcttaaa aacataatat aattacccta attgtaatgg caactctccc
     7741 tcacatcctc aaattatagt tgtcattaca aaacttttcc cataaattat aagtttatgg
     7801 gaattttttt atgttataat agtttaagcg ttaatttacg aaggagcgat acttatgaaa
     7861 ttttggaaac tacatggaat aggaaatgat tttatagcaa tagatggaag attcgaccag
     7921 ataaatccat ctgattatac tgctttagca aaaaaagtat gccatagaag attttctgta
     7981 ggagcggatg gattgttagt tgtgaaagat tcaaaagttg cagatgtaga aatggtatat
     8041 tataattctg atggttcaag agcagctatg tgtggaaatg gccttagatg tttttgtaaa
     8101 tttgtttatg ataattgtat agttaaaaaa gaaagtttta cagtagacac attagatggt
     8161 ataaaagata taaaagtaaa tcttaccaat agagaaataa actctataag agtcaatatg
     8221 ggaaaaggaa gttttatagc taaggacgtt cctgtactta caaataaaga acgttttgtg
     8281 caagaaagta taaaggtttt agatagggag cttaaagtta cttctatgtt gatgggggtt
     8341 cctcatacag tagttatagt agatgaatta gatgtagatg atgtttgtaa atatggaaaa
     8401 gagattgaaa ataataaaat atttcctgaa aaaacgaatg taaattttgt aaaggtagaa
     8461 gataagtata atatacatgt ttatacttgg gaaagaggtt gtggatatac tttaggttgt
     8521 ggtactggta tgactgcatc agcaatagtg tgcaatttac ttaatatggt agaaagtagt
     8581 gtaaatgtaa cgtctcaagg tggaacagtt aaaatagatg taggagattt ctcttatatg
     8641 acaggtccag cagtaaaaat atgtgaaggt gttttggagg tttaattaat ggctataagt
     8701 atgactggtt ttggaagagg agaatacaag gatgataatt attacttttt agtagagtgt
     8761 aaaacgataa atcataagta ttcagatata aacatacgtc ttccaagaaa aatttcattt
     8821 ttagaggata aagttagaaa tttagtaaaa aattatgtaa aaagaggaag agtagattta
     8881 tacataaagt ttgacttatt aggcaaagaa gatgtaaact taaattttga tgagggatta
     8941 gcatctcagt atatagacat actaaaagaa ataaaaaata aatttgatat tatagatgat
     9001 attagcgtta tgaatgttgc aaagtttcct gatattgtga aaatagaaga aaaggaagaa
     9061 gatgaagatt tattgtggtc aatgttgaat caagcagttg aagatgcatt aatcaaatta
     9121 agagaaatga gaagtgaaga aggtaaaaaa ttagctgaag atattgccat gagatgtgat
     9181 ttattaaaaa atcatataga agagattgaa aaatattcaa gtagtgttgt agaagattat
     9241 agagaaaaat tgaatttgag aataagtgaa ttactggatg acccaagtat aatagatgag
     9301 aatagattag cacaagaagt tgcaatatat gcagataaaa gcagtataac agaagaaata
     9361 gttagattta aaagtcatat agggcaactt aaaaatacta tatttaaaga cgactctata
     9421 ggtagaaaaa tagatttctt aatacaagag atgaatagag aaactaatac tataggttcg
     9481 aagtcatctg atataaatat aactaattta gtagtagaag taaaaagcga attggaaaaa
     9541 ataagagaac aaatacaaaa tatagagtaa gaatacaata ttagaagggg aatgcttaaa
     9601 tgtttgtaaa aaaaggatta ttgctagttg tatcaggacc atcaggtgca ggaaaaggta
     9661 ctatttgcaa agaactactt aaagaaaatg atacaataaa actttctgta tcagctacta
     9721 caagaaaacc cagaactgga gaagttgatg gagtaaatta tttcttcata agtaaggaaa
     9781 agtttgaaga gatgatagaa aaaggtgagt ttttggagta tgctcaaatt tatgataatt
     9841 tttatggaac tccaaaagcg gcaataatgg aatgtttaga aaaaggacaa gatgtattac
     9901 tggagattga aatgcaagga gcaaaacaaa taaaagaagt ttgcccagag ggagtattta
     9961 tatttgtact accaccatca cttaaagaat taaaaaatag aattgttgga agaggtacag
    10021 aaacagaagc tgaaatagag aagagattta gctgtgcata tgaagaaatt aaaatgatta
    10081 aggattatga ttattttata tttaatgaag atgtaaaaac atctgctaaa gaaatagaag
    10141 gtataatatc atctgaaaag aataaagtta gcagatataa aaatattata atagagaaat
    10201 ttaaggagga attataatta tgttaaaacc atctatcaat gaggtactag aaaaaataga
    10261 caacagatac tatttagtag ggactgtttc aaagagagca agaaaattaa ttgatggaga
    10321 agaaccatat gtttcaaata aaactaagga aaagcctgta tgtgtagcaa ctaaagaagt
    10381 tgcaagtgga aaaataacat atagattatt gacagaagag gaaatagaga tagaagaagc
    10441 tagacatcat gctgagcaac accaacaaat ttcagaagag gagtaaggtc atgctaaaag
    10501 ataaaactgt tgtaattggc ataagtggcg gtatagctgc atataaggct tgtgatgttg
    10561 taagtaagtt aaaaaaacta aatgcaaata tccatgttat aatgacaaaa tcagcaacag
    10621 aatttgttaa accacttaca tttcaatcgc tgagtcaaaa ttatgtagta gaagatatgt
    10681 ttgaagaacc aaaaacttgg gaagttgaac atatatcatt agcaaaaaaa gcagatttat
    10741 tcttaatagt gccagcaagt gcaaatgtta taggcaagat tgcaaatggt atagctgacg
    10801 atatgttgac aactactgtt atggcaacaa aagcacaggt tcttatagca ccagctatga
    10861 atactaatat gtatgaaaat ccaattgttc aaaaaaatat acaaacttta aagagctttg
    10921 gatataaatt tattgaaccg gaaagtggaa ggcttgcatg tggagatatt ggaacaggta
    10981 aactagctaa tccagatata atagtagagg aagttttgac taatcttact aaagagcaag
    11041 atttaaaagg tcaaagtatg atagttacag ctggtccaac tgtagagagt atagacccag
    11101 taagatattt aactaatagg tcttctggta aaatgggata ttctatagca agagaagcta
    11161 tagctagagg tgctgatgta acattaatat caggtcctac aaatctttct attccacaaa
    11221 atcttaaaaa atttgttcaa atagagtcag cacaagattt gtatgaagca gtagtatcaa
    11281 atttaagtga aaataaaata gtaattcaaa gtgcagcagt tgcagattat aaaccaaaaa
    11341 cttattctga caaaaaaatt aaaaagaaga atgatgattt atcaatagaa ctaagtagaa
    11401 attatgatat tgcttatgaa ataggaaaaa taaaagaaga taagatacta attggatttg
    11461 cagctgaaac taatgacctt atagaacatg ccaaaggaaa aataaaaaag aaaaatttag
    11521 attttattgt ggcaaatgat ttaactaaag aaggtgctgg ttttagagct gatactaata
    11581 ttgtaaaaat aatagatgta gagggaaata ttactgagta tcctaagatg aaaaaagaag
    11641 aagtagccaa tgttatttta gataaagtta aagagttgct tgagaaataa gacctttttt
    11701 aaatatgata tttgtgtatt ttatcacagc taaagtggct gaaagctgca atttaatttt
    11761 agcgagtaaa aactcgcaaa tgtcagttaa attgtagttt tcagcattta ttttgtcata
    11821 aagtataaaa tataattaat agttaattat taaaataaag ttaatattaa taacttgaaa
    11881 ttgcttataa attctgagat tttaatatct tgtaaagtat attaaaatat gaacaatata
    11941 ttaaatttaa gtagaggttt tataatgaaa aaatatgcaa aggtaatagt tagaagtaat
    12001 actatatata cagataattt atttacatat caaattcctg tatttttatc tgatgtaata
    12061 aaaattgggc acagaatatt agtaccattt ggaaaaggta ataaaccaac agaagccttt
    12121 gtatttcaat ttacagatag cttagatgaa aaaataaaaa taaaagaaat tattgatgta
    12181 ttagatgaaa atccgatatt tagaaaagaa gatttagagt tggtttattg gatgaaaaat
    12241 agatatttgt gtacttatat agaatgtata aatttgatat atccaaaggg atacaagtta
    12301 aataattata aagtagtcct attaggtgaa agtttaagtg gattaaatga tattgaattg
    12361 aaggaaaaaa tatcaacttt aagtgataaa aatagagaaa tagtagagaa agtgattgat
    12421 agtaaaggta aaattaaggt tgataagtta aaatatatac caaacttaaa tagttctttg
    12481 tacacaatga ataaaaatgg aattataaaa ttatgttggg aatataaaaa tcacaaaaat
    12541 gaaaagaagg tatgttatat atctttgagt ttagaaagtg ataagattga cgattatata
    12601 gagcaaaata aaataaatgt gggaagtaaa caaaaagaga tattaagttt tcttaaaaat
    12661 aatgaaaatg tagagataaa tgatttattg gatttattaa atgcttcaaa acaaagtata
    12721 aattctctaa gtaagaaaaa gctaataacc ctagaattta aggattatta tagagagcca
    12781 aaaagtattt acaaatctgt actaaaaagt ataaaattaa acaatgagca acaagaagca
    12841 gttgatgaga taaagtcgaa tatgtttgta gatgataaaa aaccatactt aattcatgga
    12901 gtaactggaa gtggaaaaac tgaagtttat atggaaataa tagaatttgc tctaaatcaa
    12961 ggcttagaca gtatattctt agtacctgag atagcattaa ctcctcaaac aatagatagg
    13021 ctaaagagta ggtttggaga tttagttgga gtgttccaca gtaaactatc tgaaggagaa
    13081 aaacatgatg tttacaaagc tgtcaaagca ggtaaagtaa gagtgttaat tggagctagg
    13141 tcagctttat ttgcaccttt taattcctta ggcttaataa taattgatga atgtcatgag
    13201 tcatcatata agtctgagaa gaatccaaaa tttaatgcta tagaagttgc tagatttatg
    13261 gcattaaaaa ataatataac tcttatttta ggttcagcaa cgccctcaat tgaagagtat
    13321 tatagggcaa aatctggaga atacaaactt ataaatataa aaagtagagc aaatgataag
    13381 cctcttccaa acattgaagt ggtagacatg aaagatgagt tagataaagg aaatagaagt
    13441 atatttagtt tgaaattaca aaaagaaata cgttatgcga ttgaagaaaa taatcaagta
    13501 atactatttt taaacagaag aggttatgca aattttgtat cttgtagaaa gtgtggatat
    13561 gtgtttcaat gtgaaaattg tgatatatct ttaacttatc ataagaaaag taatactgga
    13621 agatgccact attgtggata tgagaaagaa attccaaaag agtgtcctga atgtaaaagt
    13681 acttatgtta agccatttgg agttggaact caaaaaattg aagaggaatt aaaatatatt
    13741 tttccagata taaaaacttt gagaatggat aaagatacaa cttcaaaaaa aggtgcttta
    13801 gatgagatat taaataaatt taaagataaa gaagcagatg tactgattgg aacacagatg
    13861 ttaagtaaag ggctggattt tgagaatgta actttagtag gtattttgtc tgcagacatg
    13921 atattaaatt ttccagactt taaaagtttt gaaactacat ttcaattgat aactcaagtc
    13981 tctggaagag cagggagagc tgataaagag ggaaaagtag tacttcagac ttatgacact
    14041 gaacactatg ctattaagca tgctattgaa tatgattatg agggttttta tgaagatgag
    14101 ataaaaataa gaaaagcatt tggatattct ccttttaata atatgttaag tgtagttgtt
    14161 agtggtgaag atgaaagatt agtgataaaa aatataaaaa atatgcatgc atcattgatt
    14221 tatttattag aaaaaagggg tataaatgat ttaggattta tattaggtcc aaatccatgt
    14281 tcaatatcaa aaataaatca aaattataga tggcaaatac tctttaaaga tgaaaatatt
    14341 gaaattaatc tcttaaaggg tataataaaa tatatatgta taacaaaacg agatttgata
    14401 tttgataaga atatcaatgt atctatagat attaatccaa acagtgtatt ataaatttag
    14461 gaggaattat aatggcttta agacaaatag ttcaaatagg agaaccagta ttaagaaaaa
    14521 aatcaaaaaa ggttgaaaaa attgatgaaa agataataca attattagat gatatggcag
    14581 aaactatgta tgatgcagat ggtgttggac tggctgcacc tcaagttgga atattaaaaa
    14641 gagtagtagt aatagatatt ggagaagagc ttatagaact tataaatcct gaaataatag
    14701 aaacatctgg ggagcaaata gatgaggaag gatgtttaag tgttgttgga gaagcgggaa
    14761 atgttagaag accaaattat gtaaaggtca gagctttaaa tagaaatggt gaaacaatag
    14821 agttagaggg agaagaactt ttagctagag cattttgtca tgagatagac cacttagatg
    14881 gaattttatt tgttgacaaa atagaaaagt agtaggagtt gatttgatga aaatagtatt
    14941 tatgggaact cctgatatag cagttccatg tttacaaaaa ataatagatg agaagtatga
    15001 aatattgggt gttgtaactc aaccagataa accaaaagga agaggtaaaa aactaggtat
    15061 gagtccagta aaagaactgg caattgaaaa taatatacct gtatatcaac cagtaaaggc
    15121 tagagataaa gaatttatag ataaaattaa atctttaaat ccagatgtaa tagtagttgt
    15181 agcttttgga cagatacttc caaaagaaat attagagatt cctaaacttg gatgtataaa
    15241 tgttcatgtt tctttacttc caaaatatag aggtgcggca cctataaatt gggtaataat
    15301 aaatggtgaa gaaaagactg gtgttacaac tatgtatatg gatgaagggc tagatactgg
    15361 agatatgata ctaaagacag aagtaaatct tgatgaaaat ataacagcag gagaacttca
    15421 tgataaaatg atgaatatag gcgcagaaac tttaaaagaa acattaagat taattgaaga
    15481 aggaaatgct ccaagagaag tacaaaatca tgaagaattt tcctatgcgc caataatgaa
    15541 taaatcatta ggtaacatag atttttcaaa aagtgccaga gaaatacaca atttagttag
    15601 aggagtaaat ccatggccaa gtgcttatac tacctataat gatgtcataa tgaaagtttg
    15661 gaaaactaaa gtgttagatg aaaaaagcac aaaagatgta ggtacaataa ttgatgtaag
    15721 caaagatggg ataaaagtaa gtactataga taatgtactt ttaattgaag aaattcagat
    15781 gccgaataaa aagagaatgt tggttggtga atatataaaa gggaatacta tagaaactgg
    15841 tttagtattg ggttaggtga aaagtatgac agatataaag aagtatttaa caagtgttgg
    15901 gattttgatt ttaatattag gaattttgat aggagttgtt aattttttta taataagttt
    15961 aactcaaata ttctccttga tgataaatat tataataata tcactaatac ttataatact
    16021 atgttcaaca gcagttactt atagagtaat taaaggaaag tatgttaact caaatataat
    16081 gaaaataaat tttaatattg tcagtggatt atttcctatt atatctttca ttgcatcatc
    16141 atttggtatg tcaaaaagtg atattagaag aatatatata aagttaaata atgaatatat
    16201 atacagtaat aaatataatt ttaatccaga ggatataata attttaatac ctcattgtat
    16261 tcaagagaat agttgtaaat taaaagttac taatgatata gataattgta aagaatgtgg
    16321 aagatgcaac ataggagaat taattaagct aaaggaaaaa actaatgtaa aaatatttgt
    16381 agctacagga ggaaccctag ctagaaaaat tataatggac actaaaccta aagctgttgt
    16441 agctgttgca tgtgaaagag atttgacatc tggaatacaa gatattaaaa aaattcctgt
    16501 tttaggtgta tttaacaaaa gacctaatgg accttgtgta aatactaatg tagacatgat
    16561 ggatattgaa aaagctatag gctttttaac aggaaaaaat atattagttt gtaattaaac
    16621 tgtttttaag ttaagtgaat attaagagga tgtataataa aaatatattt taggagagtg
    16681 ttatttatgt accctgtata tggcggtttt tggggatttg acccaacaat ggttgtatta
    16741 attccagcta tattacttac tatatatgca caatttaaag tgagttcaac aacaaataag
    16801 tatttaagag taaatacacg tcgaggatat actggagagc aaacagcaag aagagtactt
    16861 gattcaaatg gtttgtatga tgtaaagata gaaatggtta gagggcatct aagtgaccat
    16921 tatgacccaa gaagaaaggc tgtaaggttg tcagaagatg tatattatgg aacatctata
    16981 acttctgttg cagtagctgc acatgagtgt ggtcatgcca tacaacatgc taaaggttat
    17041 gcacctcttc aaataagaag tagtttagta ccagtagtga attttgcttc aagtatatct
    17101 tggtttttaa tatttttagg atttattatg gctggtccat ttttaaaaat tggtatacta
    17161 ttgttttcgg cttcagtgtt attccaaata ataacattgc ctgtagaatt taatgcttca
    17221 agtagagcaa tcgttcaact tgggaattta gggattatag atgaaagtga gtcaagacaa
    17281 agtagaagag tactttcagc agcagcttta acatatgttg cagcagcatt ggtttcaata
    17341 cttcaattac ttagattgtt actaatagct caaagaagaa atgactaaaa gtaaagtggg
    17401 ttgtttagaa acaacccttt tttatgttta ataaaaggga gaaatttata tggatgcaag
    17461 agaagttgga tttaaagtat tatgtgatat agaaaaaaat gacaattatt caaatattgc
    17521 aataaataag cattttaaaa atttagaaat aagtgatatg gatagaggac ttgcaacaga
    17581 gttaatttat ggagtaatag aaaataaata ttatttagat tatataatca ataaactttc
    17641 aaaaataaag gtaaaaaaaa tgtcaactta tgttaaaatc tttttaagaa tgggaacata
    17701 tcagatttta tttttagata gtatatcaga ttatgcagca gttaatgaaa ctgtaaaatt
    17761 atctaagaaa tatgataaaa aatcttctgg tttcataaat gctattctta gaaatgaaat
    17821 aagagctaaa gagactataa tggacataac tgaggaagat agtgtaaaat acttatctat
    17881 aaagtattcc tataactctt ggattataaa aaactggata gataaatttg gacaagaatt
    17941 tgctgaagat ttgttggaag caaataatga aaagcctagt atctatataa gaacgaatac
    18001 acttaaaata agtagagaag aacttattga aaaattaagt gaaatgggta ttgtatgttt
    18061 aaaagttcct atggtagaag aagctattaa agtcgaaaaa atgaaaaata tagaaaaaaa
    18121 tgaattattt aaggctggtt tatttacaat tcaagatata agttctatga tagtagggaa
    18181 agtaattaat ccaaaagaag gttcatttgt tttggatgta tgtagtgctc ctggagggaa
    18241 atctactcac ttagctactt taatgaacaa tactggtcaa gtagtagcta gagatatttt
    18301 tgagcataaa ctaaagctta taaactctac tgtaaataga ttggggctta aaaatgtgtt
    18361 tgtagaaaga tttgatgctt ctgagattga tgaaaatagt ataagtaaat ttgattatgt
    18421 tttagcagat gttccttgct ctggttttgg tataataaga cgtaaaccag aaattaagta
    18481 taagaaagaa gaagagttaa aagacatcac ttctatacaa aagaaaattt tagaaaatgc
    18541 atctaaatat gtcaaagttg gtggaacttt agtatatagt acatgtacaa ttcaagatgt
    18601 ggagaatata aatatagtta cttcttttgt tgaagaaaat cacaactttg aatttgttcc
    18661 aattgataca gtaaatgtag acttagacaa tcaagataaa ggatatttaa agatatatcc
    18721 taatattcat ggtatagatg gttttttcat agcaaaatta aagagaataa ggtagtgaga
    18781 gaatggaaaa gaaaaagata gtattaaaaa attttactga agatgaactt aaagaattta
    18841 tgaaaactat agatgaaaag ccctttagag gaagtcaaat attctcttgg atatataagg
    18901 gtgcaaaaac ttttgatgat atgaacaata taccaaaaag tttgagaaat aaattagaag
    18961 aggtttcatg tatagggcat atagatatag aattaaagtt agaatctaag gtagataata
    19021 caaaaaaata tttgttttta ttagatgatg gaaatataat agaaactgtg atgatggatt
    19081 atgatagtag agttactgtc tgtgtttcta accaagtagg ttgtaggatg ggatgtaatt
    19141 tttgtgcatc tacaatggat ggattaatta gaaacttaga gccatgggaa attttagacc
    19201 aagttattaa aattcaagaa gatactggta agagggtatc aaatcttgta ttgatgggga
    19261 gtggtgagcc acttgataac tttgaaaata caaaacaatt tttaaaaata attaacgaaa
    19321 aaaatggtct taatataggg tatagacata taactctttc aacttgtgga atagtaccta
    19381 aaatgtatga gttggcagac ttagaaatag ctataaattt agctttatca cttcattcac
    19441 catatgatga agaacgaaga aaaattatgc ctgtagcaaa tgcttattct attgaagaaa
    19501 tattgaatgc atgtagatat tatataaaaa agacaaatag aagggttaca tttgaatatt
    19561 ctcttataaa aggggtaaat gactcagaaa aagaagcaaa ggcattagct aagctcctaa
    19621 aaggtatgct atgtcatgta aatttaattc caataaataa agttgaagaa agagaatatg
    19681 aaaagccaga taaagcattt atttataaat ttagggatag tttggagaag aacaatatac
    19741 ctgccacagt taggatgtct atgggttctg atattagtgg agcttgtggg caattgagaa
    19801 gaaaatacaa gtaatttgta aggaggaagt cgtatggttt atagttgtgc ctcccatata
    19861 ggaaaaataa gaaaaaacaa tgaagactat tgtgagggag aagttataga tacagaacat
    19921 ggtccaatag gaatatttgc catagctgat ggaatgggtg gacataaaaa aggtgaagtc
    19981 gccagtaaat tagcagtaga aaatataatt gattttctaa aggaaaactt attacaacat
    20041 gataatgtaa aaatagacta tatagatgat atattaaaac aggcttataa taatgtaaac
    20101 tcaattgtgc acaaaaaatc tatggaagat atagaatttg aaggaatggg aactacatta
    20161 gttacggcta tagtgtataa taatgtctta tatgtggcaa atgttggaga tagcagatgt
    20221 tatttgttaa cagatgaaaa atttgataaa ataactatag accattccgt ggttgaagaa
    20281 cttatgatgg cccatgtcat tacagaagaa gaggcaagaa gacatccaca gaggaataga
    20341 ataactagag caattggaac agatgatatg gttgttgtag atatttttaa gaaagaaatc
    20401 aaaaaaagtg atataatact tcttgcgaca gatggattaa ctgggtttat tgatgatgaa
    20461 gatataagag atttaatatt agattatgaa tatgagagaa ctagtaatat aagtgaagaa
    20521 ttgataagta tggccaatga tgtttctggt aaagataatg tttcggttat agtgataaaa
    20581 gtataattag gacggtgata ttgtgggaga tacaatttta ggaaatcgat atgaaatcat
    20641 caggaagatt ggtgatggag gaatggcctt tgtatatgag gctaaagaca gattattaaa
    20701 caggactgtt gcacttaaag tacttagacc tgaatttgta gatgatgatg aatttctaac
    20761 aaagtttaaa agagaggcag aagcagtagc aagtctttca catccaaata tagtaaatgt
    20821 ttatgatgtt ggtgaagatg gaaaagttca ctacattgta atggagtttg tagatgggaa
    20881 gaatctaaaa gagattatac aagatgaagg tatattagat gaatatactg cattagatat
    20941 aacaaaacaa atagctatgg cacttagtgc agcgcacaaa aaggggatta tacatagaga
    21001 tataaaacct cataatattc tcatatcaaa tgaaggaaga gtagtaaaag tggctgattt
    21061 tggtatagcc aaagctgttt caaattcaac aatgactaat attggtagta taataggttc
    21121 agtgcattat ttttcaccag agcaagctaa aggtaaattt gtaactaata atgctgacct
    21181 gtattctcta ggtatagttt tatatgaaat gttgatagga aaagttccat ttaggggaga
    21241 tagtccaatt tctatagcac ttcaacacat taatgatgat atagacttta catcagaaga
    21301 aaaagttagg attcctcaaa gtgtaagaac tacaataaaa aagcttacag aaaaatctag
    21361 tgcagataga tatcaaactg ctgaagaatt gatagaagat atagagtata tagaaaaaaa
    21421 tattgattta gattttataa aagaatatga tgattttgca actaagaaaa ttgatgaaaa
    21481 agaaattaat aaagttgtaa atccaacact tgcaaaacca gctcctgaaa aagtggttaa
    21541 gcctgttgaa gtagctgatt tggatgatga tgaagattac tatgatgatt tttatgaaga
    21601 agatgatgat gaagatgaag aagaggaaga gataatgaga gctaaaaaga atcagagacc
    21661 taagagtact ccaagtaaga gaactaagaa aaagaagaaa aaacaggaaa gcccaaaatc
    21721 tagaagaaga ttaaaagtaa ttgcggctgt tttgatactg atattgtgtg ctcaagtatt
    21781 cttagcatat aagtttttat ttgctggagg gtttggcaat aaatctttga ctgttcctaa
    21841 tcttgtaaat atgacattag aagaagctca aagtgctgtt gaaaaagaag gattatacct
    21901 gagtgtaaaa agtgaagagt ataatagtga agttgatgaa aattgtataa tatcacagac
    21961 acctgaaggt ggaagtacaa atgtcaaaaa gggtgatact ataaatgttg tcgtaagtaa
    22021 aggttcaagt caggcatctg ttcctaatgt agtgggactt actttgagta atgcaaaaca
    22081 acttattgaa gaaaataact taaaagtggg aactgttaag tatgaataca gctcaatata
    22141 taaagaaggt acagttttaa gtcagagtcc gggtgctggc tcatcaagag ctcaggaagg
    22201 tgatgaggta aacctatatg taagcaaagg ttcagaaaaa tcaaatacac agactccaac
    22261 agtaccagat aaaaaaccaa caacaccaga gaataataca ccaacagaac ctggttcaaa
    22321 ctcaggaaac agtggtggaa attcaggagg ctcaaattca ggaaatagtg gtggaaactc
    22381 aggcaataat ggaagtaact caggaggttc aaactcagga aatagtggtg gaaactcagg
    22441 aggctcaaac tcaggagata atggaggaag ttcaggaggc tcaaactcag gagacaatgg
    22501 aggaagttca ggaggttcaa actcaggaga caatggagat agttcaggag gctcaaactc
    22561 aggagataat ggaggaactt catcaggaac tggcgcaaac attggtaaaa cagaataaat
    22621 attaataaaa taagagctgt ccttttatag aaaatatttt ctttaaaggg cagtttgttt
    22681 tgtataaaat actgaattgg ttataatata aggagaaaag tatttatgaa gaagaaaaca
    22741 gttttaatta caggaggagc tagagggatt ggtaaagcta tgtctaaagc ttttgcaaaa
    22801 gaaggttaca atgtattagt aaattttaat aagtcagaaa atgaagctaa agagttgtac
    22861 actatattaa atgaaaaaaa tttttctgta aaacttttta aggctgatat ttcaaataga
    22921 gaagatgtag aggatatggt agattattgt ataaaagaat ttggaggatt ggatgtactt
    22981 gtaaacaatg ctggtgtaag tcaagataag ttgtttacag atataactga tgaagattgg
    23041 gataatatga tgaatattaa tttaaaagga agcttttatt gttcacaagt tgctttaaaa
    23101 tacatgatat cagaaaaaaa aggaaatata attaatatat cttcaatctg gggtatatca
    23161 ggtgcgtctt gtgaagtgca ttattctata acaaaagcag gcataatagg tatgacaaag
    23221 gcattagcta aagaagtagg accatcaaat attagagtaa atagcatagc tccaggtgtc
    23281 attaatactg acatgttatc tggatataat gaggaagata tagacgctct tgtggaagaa
    23341 acccctctaa tgaggctagg aactccagaa gatatagcaa attgtgctat tttcttggca
    23401 tcagacaaat caaattttat cactggtcag gtaattagtc caaacggagg atttgttatt
    23461 taaaaaaaat gtggtattat ataaaaatag gaaagaataa cacgatatac tactcataaa
    23521 cagtatgtgt ttatataaat tttataaagg gaggcatttg atgctagaag gaaaaattat
    23581 aaaggggatt agtggatttt attatgtaga tacttataat ggtatttatg aatgtaaggc
    23641 tagagggata tttagaaagc agaaaataac acctttggtt ggtgatagag tcaaaataag
    23701 cattgttgat gaagatgaaa aaaagggtat cttagaagaa atcgacagta gagatacaga
    23761 acttataaga cctcctattg caaatgtaga taaagcatta atagtattcg caataaaaaa
    23821 tcctaaacca aacttatctc ttttagatag atttatagtt ttggcagaaa aagaaaattt
    23881 agaaactgta ataatcctta caaaagcaga tttagatgat aatgatacat tagaaactgt
    23941 taaaaatata tatgaactta gtggctataa agtcattcct gtaagtaata ttactaagtt
    24001 aaatatagat aaagtaaaag aagaattaaa agaaaatgta gtagtgtttg caggaccatc
    24061 tggagttgga aaatcaagct tattaaatga aatagatgaa aattttaaac ttcaaacagg
    24121 agttgtaagt gataaaataa aaaggggaaa acatactact cgtcacgctg aacttttaaa
    24181 attagaattt ggtggaatgg ttgcagatac accaggtttt agttcccttg tccttgaaga
    24241 tatagaggaa gttgaactta aagattattt tatagaattt gataaattta atgattgtaa
    24301 gtttggttca aagtgtattc atgaaaatga acctaattgt gctataaaag aagctgttac
    24361 aaatggagaa atatctaaag aaagatatga tagttatata cagctgctac atgaaataag
    24421 acaaaataat agtaggaggt attagaaaca tgattaaatt agcaccatca atattatcag
    24481 cagattttgc aaaattatta gaagatgtaa gaaaagtaga aagtgcagga tgtgaatatc
    24541 ttcatatcga tgtaatggat ggacactttg taccaaacat tacattagga ccacttgtag
    24601 tcaaaagttt aaagaaagaa aatataaata tggtatttga tgctcatctt atgatagaaa
    24661 atccagacca gtatattgaa gaatttgtaa aagcaggatg tgatataata actgttcacc
    24721 aggaggcttg tgttcatcta catagaacta tacaaaatat aaaatcacat ggaataaaag
    24781 ctggtgtagt acttaatcca gctacaccag tagatacgat taaacatgta ctacctgatt
    24841 tagatatggt attattaatg tctgttaacc ctggatttgg aggacaatca tttattcctt
    24901 gtgttttaga taagattaaa gagttaaaag ctattataga tagtcaagga ttaaacattg
    24961 atatagaagt tgatggtggt ataagcccta aaaacgtagc cgaagttgta caagcaggag
    25021 ctaatgtaat agtagcaggt tctgcaatat ttggaagtga tgacattcaa gaaacagtta
    25081 atttatttag aaagaatgct tctcttgaag aattagttta aatttaataa tgaaaatctg
    25141 tattgtttta aatggtgaaa ttgaagatta cgaagttaca agagatatta taattaagga
    25201 atgttatgac tgcataatat gtgcagatgg aggggcaaat cacacttata aaatggaaat
    25261 aatgccagat tatatattag gagatttaga ttcagtagaa gaagaaaaga taagcttcta
    25321 taaaaataaa ggtgttaaat ttgaaaaatt tccatctaaa aaagatgaga ctgatacaga
    25381 gctttgtctt tttttagcta agacacttaa agcaaatcat attgattttt ttggtgcttt
    25441 agggggaagg attgaccaca ctcttgcaaa tataaagcta ttatattatt taaaagaaga
    25501 tggtatatat tctaggatac tttctgataa agaagagatg tatatagttg aaaatgagga
    25561 aatttcattg tatggaaacc caggggacac tatttctgta atagctataa atggtgatgc
    25621 taagggtgta acacttactg gcttagaata ccctctcgat aattattata tgaaatactc
    25681 tgtacctatt ggaatatcaa atgttatgtt gagtaattct tgtaagatta aagtagagca
    25741 aggctgtgta cttgtagtta gaaatttata aaatttgatt ttgacaattg gttttatatt
    25801 gtagagcaga tacaatcaag taaaatatat ttatgattgt atctgctttt tagtatataa
    25861 ttataaatag ataataaact ttgacataag ggggattttt tgtggtagat agtattattt
    25921 ttgatttaga tggaacattg tgggattcaa ctgaagaagt atgcaaagtc tggcaggatg
    25981 ttttaagtga gcataaggaa atagaattaa gtgtcacaaa agatttattt agaagtctta
    26041 tggggctttc ttttgaggaa atagcaaatc gtttatttcc aaatttaagt gaagaagaaa
    26101 gaatgaattt attgaatgaa tgttctttga aagaatgtga ctatttatct gaaaatggag
    26161 ctaaattatt tgatgatata gaagatactc ttaaattact atctaaaaaa tataaattat
    26221 ttatagtaag taattgtcaa gctggatata tagaatcttt tttaaaagca cataaactag
    26281 aacagtattt tatagatttt gaatgtcctg gtaatactgg tttgcataaa ggagaaaata
    26341 ataaacttat aattgaaaga aataaattaa taaatccaat ctatgttggt gatacgcaag
    26401 gtgatgcaaa tagtgctaag tttgtaggta taccttttat atatgccaaa tatgggtttg
    26461 ggaatgtaga tgtatatgat tattttatag actcatttaa agatttgtta gaacatgata
    26521 tattaaaata agatgagttt aaaaattata ttaaaataat aaaaaagtta tggggggatt
    26581 taaaaatgga attacaagat actattttta aaagacaaag tgtaagaaaa tttaaaaatc
    26641 aagatgtatc agatgaagat attttaaaaa tgataaaagc agcaggtgca gcaccatcag
    26701 gcaagaatat tcaaaactgg cattttgttg ttataaaacg ccgtgattta attgaaaaga
    26761 tagcagatgt gataactaaa aagcaacaag aaatacttgt agaaatggat aaagtatcag
    26821 tggataaggc taatagattt agaaaatttg ttaaaaattt tacactattt tacttaaaag
    26881 ctccagtttt agtattagtt tttacaaagg tatataatcc atcaggatat tatgaattag
    26941 agcttataga tgcacctaag gagactatag ataaattatt tataagaaat ccaggtatgc
    27001 agagtctagg tgctgcaatt gaaaacttta cactatcagc aattgaactt ggatatggtt
    27061 cttgttggtt gacaagtcaa aactatgcag cagatgagat agaagctgtt ttagaagcag
    27121 aaactggttt tgaaaaggga gagtattttt taggtgcaat gttagctctt ggagtgccag
    27181 aggacaattt gaagagtcca tctaaaaaac cagtagaaga aatatgtaca tttataaaat
    27241 aaaagtcaag gacattttaa aatcgaagta aattaaaaac aaaataaatt aaaaataaat
    27301 atttaaaaaa ttaaaaagta ggttttattg atattaattt tatatttc
//
LOCUS       DBGJFF010000047        27227 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.047, whole genome shotgun sequence.
ACCESSION   DBGJFF010000047 DBGJFF010000000
VERSION     DBGJFF010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27227
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.047"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(96..1052)
                     /locus_tag="ACOIAF_001961"
     CDS             complement(96..1052)
                     /locus_tag="ACOIAF_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HGZ2668971.1"
                     /translation="MSGLKKFVIFLALLVIAFPVSIYGYVFTKLKTIQDDSINTEALS
                     SNDYKNEENITNILLLGTDARPGEEVSRSDAMMILTIDNKNKSLKLTSLNRDTYVNIP
                     GRGEQKLTHAYVYGQANLLIETIEENFQLDIQNYAVVDFFSFMDIVDALGGVTVTVDK
                     SEIRELNKFIPETYKWNKNDNKGSLKYIRNSGEQKLNGYQTLAFSRIRKNDSTDERDR
                     RQRSVIQSLINGVKDLPVTKYPNLVNTILPYVKTNMNPNEIISLGKELLSIGNLNLKS
                     MEFPLSTENGRKIGNAGYVIPFEEYELDAMHDFIFKDIMVED"
     gene            1420..2208
                     /locus_tag="ACOIAF_001962"
     CDS             1420..2208
                     /locus_tag="ACOIAF_001962"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HGZ2668972.1"
                     /translation="MIKLIATDLDGTLLDEKSEINPEFYKVFKKLRERGIMFSAASGR
                     QYQNLIKKFEDIKDDMMFISENGTLVVYKGKEILSNPLNKELVNEIIETTRSIKGKKI
                     VMSGKKYAYIESKDEAFIQEVSTYYAKFKIVEDLTKVEGDILKIAVFDFKGAEHNNNI
                     YFEKFSDRAQVCISGVEWLDLTAKGANKGSAIKKVQKMLDIKYEETMVFGDQLNDVEM
                     MKSAYHSYAMENANEHLKQIARFRAKRNTENGVVDKIKEVIKIG"
     gene            complement(2331..2801)
                     /locus_tag="ACOIAF_001963"
     CDS             complement(2331..2801)
                     /locus_tag="ACOIAF_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4358 domain-containing protein"
                     /protein_id="HGZ2668973.1"
                     /translation="MKKILILFFSIILSLGMVSCSSSGDYKNVSTSKIKKALKSSDLL
                     IKESKIFDAKDFNYFNDVEEDIIQGFVINSSEKLSLEDIIVIKTDEVDKIYKTLENYK
                     KDMIQAPFGEGHGEKINSEIANNTIIEKAGRYVYLISAENATQIENKILNIIKK"
     gene            2940..3584
                     /gene="uppS"
                     /locus_tag="ACOIAF_001964"
     CDS             2940..3584
                     /gene="uppS"
                     /locus_tag="ACOIAF_001964"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HGZ2668974.1"
                     /translation="MRIPKHVGIIPDGNRRWASQNGMTKDKGYESGIDPGLESYRLCR
                     DLGCEEVTFYGFTTDNTRRPSVQTKAFTKACIDAANILIDEGAALLVVGNTKSPMFPE
                     ELLPYTNRIKAKEGQIKANLLVNYGWYWDLNNISGVENVNKNNIQDYIQSFDVSRLDL
                     IIRWGGRRRLSGFLPVQSIYADFYVIDNYWPDFKSDDIKDAFSWYDTQDVTLGG"
     gene            3708..4532
                     /locus_tag="ACOIAF_001965"
     CDS             3708..4532
                     /locus_tag="ACOIAF_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HGZ2668975.1"
                     /translation="MSKGNNNNNSRNKSKKTSHLNRKKRKLNKKKLAVLICFTVLFLF
                     IAFKATQGVVALVKSMDKSNKTNQQQNVNSEQFDLGNEEENKKKKYTVFIDPGHGGND
                     KGTESKTSNRYEKDLNLQIAKKLANKLSKQKDIQVVVSRTDDTYISLKDRAILANNSS
                     ADVLVSIHLNAEKNGNTATGIETWYRNKATDGSKELAQTVQSTIVSYVKVRDRGIVEN
                     NFEVLRESNMPAILIECGFLTTPSEEQKIINEKYQDQLAEGIVQGVLSYLDSKGNK"
     gene            4847..6394
                     /locus_tag="ACOIAF_001966"
     CDS             4847..6394
                     /locus_tag="ACOIAF_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HGZ2668976.1"
                     /translation="MNKNSMTRIYSLNKIIFIIIGILGIVLLTRHIEVDIKVLILILL
                     TLFSINVFISYLKIKLYEDKINLGLINNSDKFINITKDENFLKHVQNYIISNEKENCV
                     MACFDLCRLKLINDIYGYELGDEVLNNILSNLKNYFGKEAIYGKLKSDVFVLIIKLEN
                     REQKIPYLVNLIKNKIVILSQIDSFKMDINIEASIGIYKFNNNEIDIKKAIDNADMAR
                     LKSKGLKHIEYVEFDNAMEEEIQSIMKIERDLFLAIKNKEFVIHYQPKVDSSTGNIIG
                     SEALIRWLHPSLGMVGPNKFIPIAEKNGLINNIGRWLIQEVFITINKWISEGINVLPV
                     SINLSRVELYKNDLVDFFKLMFNTYNIPKELIELEITETTALRDVKFISERLYEIKSL
                     GMNISLDDFGVGNSNFINLKGIPLDIIKIDRSLILDIVTNTKTKFMVKAIVNLSHDLN
                     VTVICEGVEDMHQVKVLSELGCNFIQGYVFFKPLDEMNYKKLLTDGSYINLKDTLLDK
                     CMTVEEE"
     gene            6397..6795
                     /locus_tag="ACOIAF_001967"
     CDS             6397..6795
                     /locus_tag="ACOIAF_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HGZ2668977.1"
                     /translation="MISEDVDYSIRIIRAIFNNGEDKPIDAKMILKSEDIPILTLYRL
                     LRKLCANNILTFNKEDGKTYMLNVSLDTISLRTIVEVIDGKIYINKCTRYVSNCSNCL
                     KGCKVNRELLEIEKEIYKLLSRKSIRQILE"
     gene            6835..8568
                     /locus_tag="ACOIAF_001968"
     CDS             6835..8568
                     /locus_tag="ACOIAF_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HGZ2668978.1"
                     /translation="MDDISQDNFLLSKEYENSLDVDTKKASGIYYTPKIIVDYIVKKT
                     LKNHDIIKNPYPRILDISCGCGNFLLEVYDILYDLFEENIYELKKKYDENYWTVDNIH
                     RHILNYCIYGADIDEKAISILKDSLTNKKVVNDLDESDIKINLFCCDSLKKKWRYKFD
                     YIVGNPPYIGHKKLEKKYKKFLLEKYSEVYKDKADLYFCFYKKIIDILKQGGIGSVIT
                     PRYFLESLSGKDLREYIKSNVNVQEIVDFLGANIFKNIGVSSCILTFDKKKTKETYID
                     VFKIKNEDICINKFETLEELLKSSKFEHFNINQRLLSDEWILVNKDDETFYNKIQEKC
                     KYSLEDIAISFQGIITGCDKAFILSKDDVKLNLVDDKFLKCWIKSKNINKYIVDKSEY
                     RLIYSNDIDNENTNKRILDEIIGLYKTKLENRRECKSGIRKWYELQWGREKLFFERKK
                     IMYPYKSNENRFAIDYDNNFSSADVYSFFIKEEYLDKFSYEYLVGILNSSVYDKYFKI
                     TAKKMSKNIYDYYPNKVMKIRIFRDNNYEEIENLSKQIISILLNKSIDKGKVEKLQIK
                     MDNLIMDSLGI"
     gene            8800..10011
                     /locus_tag="ACOIAF_001969"
     CDS             8800..10011
                     /locus_tag="ACOIAF_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HGZ2668979.1"
                     /translation="MKGLVLEGGGTKGAYQIGAYKALRELGIEFQGVTGTSIGALNGA
                     YIIQNDIEVMEDIWLNYDYTHFMNIDRETYERFKNVDFTTKNLNAVFELINKARKNEG
                     IDISPLRQLMETTLNEDIIRKSKKDFGLVTVWWDKKINPYPLYIEDIPKGRLIDYLIA
                     SSSLPIFQTNMMDDKLYLDGMFCDNMPIGLLEDKGYEDVVVIRLIDDFIGKMNLNRHQ
                     NINIKTIIPSEYLGGSLNKDRDNVEKNIRLGYLDTMKAYERCTGIKYYFDVEYKYNEE
                     YCFDKFRSMSNETIQYICNLLNIKRDASLRTVMESVIPKLGEVLGLPKDFSYKDLFYC
                     IYEKKLEENNINRIKLYDFNKVIDVVNTNIDSNRNLASNFEVRPKISIKKNKNTKLLT
                     NCIIKDIKNQS"
     gene            10204..10464
                     /locus_tag="ACOIAF_001970"
     CDS             10204..10464
                     /locus_tag="ACOIAF_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HGZ2668980.1"
                     /translation="MEKVVSIKNASGLHARPAGMFVKKAAEFKSTVEVVAKDKTVNAK
                     SIMGIMSLGLAQGEELKLVVNGEDEEVALAAMVELVESGFGE"
     gene            10818..12530
                     /gene="ptsP"
                     /locus_tag="ACOIAF_001971"
     CDS             10818..12530
                     /gene="ptsP"
                     /locus_tag="ACOIAF_001971"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HGZ2668981.1"
                     /translation="MAYKGIGASPGVALGKALVVEHSELVIEKKSIDNVEAEIAKLED
                     AVAVSKEELVKVKEKASEELGAEEAEIFEAHLLVLEDPELIGSAIDKIKTESVNAEYA
                     LNEIKEMFVSMFESMDNEYMKERAADIKDVTNRILRHILGIKVVDLSALSEEVVLIAH
                     DLTPSDTATMNKKMVLGFLTDIGGRTSHTAIMSRTLEIAAIVGLNDITSKVKDGDFVV
                     FNGDTGEVIVNPDEETINKYTELKAKYEDERKALQLLKGKPSVTLDGKHVELAGNIGT
                     PNDIEGLIKNDAEGVGLYRTEFLYMDRDSFPTEEIQYEAYKAVLEGMDGKPIVIRTLD
                     IGGDKELSYLSMEPEMNPFLGYRAIRLCLDRKDIFKTQLRALYRASVHGRLRIMFPMI
                     SSLEELLQAKEVVKEVLAELDSEGVAYAKDVEIGMMIEVPSAAVISDVLAKHVDFFSI
                     GTNDLIQYTCAVDRMNQKISYLYNQFNPAVLRLIKTVIDNAHKEGKWAGMCGESAGDQ
                     KMIPILLGMGLDEFSMSPISILPARKLITSVKESDMKKLADDVLNMGTAEEIKSYIEK
                     TFNI"
     gene            12853..14403
                     /locus_tag="ACOIAF_001972"
     CDS             12853..14403
                     /locus_tag="ACOIAF_001972"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HGZ2668982.1"
                     /translation="MRYKKRNIDKSSELSFQSLAKYFITFYIVIILIILSMFCINTIW
                     VMKKDNQDLLSTTSNRIEDRVGETIKLLNSLSKDNYIIDNSVNGIDKSKELSMYSDEF
                     GYMMICFVDKNIIVWDEEGGNISLAGRDYMQKLFATKKYQVTDSFAAGADGKTLNYTI
                     ASPIIKNGNVDGCVFAAIYFDEIKDILKQGKGQQQQNFILFGSENQIMNLTSDETAYG
                     EKYMKLSQKTKYLGTNAKKVEKDMFNNKSGDYWAFENGKLYFMSYKPIKNTNWSLFCK
                     VNFLDIFALNLIPMGAILSVITLVFIILICMEKSFMKKRMEVIDMLLGSVQELEKRIY
                     QTERPSEVDFKEIIQLTSKGLTDGLTGVVTRTVFFDQIYNRVEMEENDKLSVISFVDL
                     DDLKCINDLYGHEIGDMVLKNVGYVLREYEIKYNGLIGRYGGDEFVMLLSGFTSREEV
                     EDLLDEIAIRLHTEVLTDDGIIQTHCSVGAVVCSVKDNKDIDNLLKQADEALYYVKRN
                     GKGNYHLV"
     gene            14429..17131
                     /locus_tag="ACOIAF_001973"
     CDS             14429..17131
                     /locus_tag="ACOIAF_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HGZ2668983.1"
                     /translation="MKNVSKKFFVCTIIVLITFLCFSKFAIYVFDKQEKKETKVVENM
                     LNVYKNNIILNLTEKFNYSKDLASVLVSNNYSIDKLKPIADEILKNNSCIYISVVEKY
                     NVAAFFSNSVKKDIINTPIQELNYSYSLANSIKGLVVEGPSKDQFSNGDVFLITNPIY
                     KNGEYWGEITVALDAKKVVDSFNLNKLKESGFEYELWRVHLDNGSKSTIEISTKDFNF
                     SKSHEIKFKLPTVWTLSIVYKNGWNNKNIDFIVFVILLIISILIGVMTFIYIEKKSIS
                     KELNQQSLKNLETGILNREGFLIKLQEHLNRKNNEIVLLYICITNYSKFAPVMSFEKR
                     QDYLTHIVKNLDNYIKSSHFIARISEEGFVLALEENCDKNELLNIEKGISLELLRKIK
                     VENKQVFLTIGYGSSRCVDDRKDAESLLLSAIEDYERRFPAMSPIHDFTKKCEDMLDE
                     KKQIVFGEYPDLEINKLSKVIYKYYKKVQRIMYKDYNIDIGNRRGYMRDVEVLIDYNS
                     ERMLYLFLIDICKFGKFNELFSIEIGDVILKEVSNKLKTIFSNYLYRINGDVFLGISI
                     DKESYLDTLHKVNEVFNSSLSVEDVLFDVKCNIGICQYPYHGENPYDLLEKVQIALEY
                     AKKDVKSNVKVYDKELTEEIYHENNIIKDLKKGLEEQTLEVWYQPIFNVSKNCYIKAE
                     ALIRLRNKEGGYYSAYEVITYAEKHGLIKDVGLYVLKKACDVTIKIKDKNIDFEQMQL
                     NLSVQQIVHKQCANDILSCIENCGLDKKYICVEVTETVLMETFEIVNVTLDKLKKEGI
                     SIALDEFGSGFSSINYLANLPIENLKIDKELVEQITYSKKQFELLKTIVQIAKINGMD
                     IVAEGVETKETLDLVVTSGVNLIQGHYFSEPLSEDKFIELMIEE"
     gene            complement(17637..18302)
                     /locus_tag="ACOIAF_001974"
     CDS             complement(17637..18302)
                     /locus_tag="ACOIAF_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668984.1"
                     /translation="MKGNIVQYNFADIEEEVYSLDYAIAWNTDEENVNIIPFTNKFCK
                     ESIESFCLGKINNFVEILNEGFVENHHYVHLDKMISVPKKKVTLVYQQDTHGYLLRDD
                     NDNLIPAKITSEQSKSISSKMELFCAGEEKCLINILLKADPSYILDVDSIKDKNILNL
                     GYESIDRYKEYNFDDDKILIFFINKKRYSVIMKKTNNSDNDLVSRNNAIKELFTNKAG
                     NLN"
     gene            18646..20076
                     /locus_tag="ACOIAF_001975"
     CDS             18646..20076
                     /locus_tag="ACOIAF_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HGZ2668985.1"
                     /translation="MDIKNKIKSIFLPEKNDYLDDEEFEYDIENNEEDLKEDNKVHLN
                     DLSRVKYDYRELKDIENQTEEMCLLAVKQDGTIIEFVKDKTYKVCMEAVKQTYKSLKY
                     ITNQNEDICIEAVKQNYRALYYINNKTENVLIEAIKNASTYDVMEVFKFVEEQTEDVC
                     LAFIERASKNDVAEILKGIKEQTPAICLEAVKKDGKSLAYVKEQSNSICLEAVKENYS
                     ALSCVKEQTEEICIEAVKQNDFALYYVNEQTEKICMEAVKRSYMALQYVNKQTEEICL
                     EAVRIDGRALQYVKEQTEEICLESVRQNGKVLQYVKKQTENICIEAVRGSFEELEIKE
                     ILSYVKIPTERIFVEAVKQNGKILKYVENQTELICLEAVRENYNALAYVKEQTEKICL
                     EAVNQSYEALKYVKEQTEEVCLKAVKQDYRMLKYVNNQTEKICLEAVKQNYRALEFVD
                     NQTEKVCLEAVKQNRKALQYVKQKQS"
     gene            20444..21022
                     /locus_tag="ACOIAF_001976"
     CDS             20444..21022
                     /locus_tag="ACOIAF_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator ArgB-like protein"
                     /protein_id="HGZ2668986.1"
                     /translation="MFKRYAEKMTSVLICNNMIDNNESKVYSYGFEILIAFIVNITTM
                     LFIGFLFGKFTYVLFFLMCYCPIRQFSGGYHADNYFRCLLTFIFIILSTILIIENINI
                     DLFKNIIMIIASVSWVGICVLCPIEHRSNPISDREKLVYKKTAIFISTVVLLITILSL
                     SLSIFVDYFTYSAFAMFWIFMMLVLGKLKAKV"
     gene            21052..21198
                     /locus_tag="ACOIAF_001977"
     CDS             21052..21198
                     /locus_tag="ACOIAF_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HGZ2668987.1"
                     /translation="MKKFIVRFMKFASSLALSTAILSANSTCPWIIHQPKVPKEISNL
                     KKTN"
     gene            21503..22882
                     /gene="scfB"
                     /locus_tag="ACOIAF_001978"
     CDS             21503..22882
                     /gene="scfB"
                     /locus_tag="ACOIAF_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HGZ2668988.1"
                     /translation="MSMIHKFSMNGYNIVLDVNGGAVHVLDDVAYDLLDFYKEKSKEE
                     ILEILKSKYQEEKINEAYEEILNLEKEGLLYTEDTYQYHPSFVHREPVVKALCLNVAH
                     DCNLKCKYCFAAQGDFGGEKELMSFEVGKAAIDYLIANSGSRKNLEIDFFGGEPLMNF
                     EVVKQLVDYGRSVEKDYNKNIRFTITTNGVLLNDEIIDYINENMHNVVLSLDGRKEVN
                     DNMRPTLNDKGSYDITLPRFKKLVEKRAKDKYYYIRGTFTRDNLDFSKDVMHFADLGF
                     KLTSVEPVVGDESNPYALREEDLPKIFEEYEKFAVEYADRQLQGDGFKFFHFMIDLNQ
                     GPCVIKRITGCGAGNEYLSVTPNGDIYPCHQFVGNEEFKMANIFDEEIVLPENLKNMF
                     REAHVYTKEECKQCWNKFYCSGGCHANAINFNSDISKPYELGCEMQRKRTECSIMIQA
                     KLMLEGATN"
     gene            22911..23408
                     /locus_tag="ACOIAF_001979"
     CDS             22911..23408
                     /locus_tag="ACOIAF_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HGZ2668989.1"
                     /translation="MIRDYLEDKPLIDESVFVAKSADVIGNVKIGKDSSIWYNAVVRG
                     DEGPITIGENTNIQDCSIVHGDTETIIGNNVTVGHRSIVHGCKISDNVLIGMGSIILD
                     NAEIGEYTLIGAGTLITSNKKFPPGVLIMGSPGKVVRELTEEDKKYIDESYEWYLEAA
                     QNQKY"
     gene            23706..25370
                     /locus_tag="ACOIAF_001980"
     CDS             23706..25370
                     /locus_tag="ACOIAF_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC1 kinase family protein"
                     /protein_id="HGZ2668990.1"
                     /translation="MRISYRNLKRYREIGYVLIKYGFSFIVERLNIEGIAYKIPLFDP
                     PEEIKNMTTGERMKRVLEELGPTYIKIGQILSTRKDLLDQDIIDEISKLRDDVEKFDS
                     NIAIDIFKEEVGLSIEEIFLEFKEEPIAAASIGQVYEGVLKTGEEVIVKIQRPNIEKI
                     IKSDLEILRTIANTLKDLKKDFNLDLVQMIEEFQTQLMRELDYTFEAINATKFSRIFK
                     NSDEVYIPKVYSEYNTKKILVMEKVNGTKLSDVEKIRRLGYNTKTIVEIGVRSFFTQV
                     LSHGFFHADPHPGNIFVVAKNKIAYIDFGMIGIIDNKTLNQLNEIALAGVEKNVDKII
                     YLLIEMDALNGEADIKGLRQDLLYLIHYYYDISIEKINVTDILNELFRFFRQYKIVMP
                     AQFVTLAKTVITLEGTSRTLNTDFSFGSMGKEFMKHHYKSKFNPKNVVLSSRQNVEEI
                     LLDIKTIPKQLKAILKNIERNNIKMQIEDVKMTRLENCIIELTSQISLSLVLASIIVG
                     SSLIIASPNIENNIWIKFTAIAGFFISFIIGLCLVIRSIRSKYKKD"
     gene            25383..>27227
                     /gene="recJ"
                     /locus_tag="ACOIAF_001981"
     CDS             25383..>27227
                     /gene="recJ"
                     /locus_tag="ACOIAF_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HGZ2668991.1"
                     /translation="MKKWILKYKGKIEESEFSKKLNISPEICQILKNRGICTEKDSEI
                     FMNPSLDYLRDPFLMKDMQKAVDRIKLAVEKNEKIWIYGDYDVDGVSSTSILCLYFKS
                     IGYNVDYYIPNRLEEGYGINEDAIKLISSRGCDLIISVDCGITSVSEVNTANDLGIDV
                     IITDHHECQSEIPSAFAVINPKQEDCNYPFDSLCGCGVAFKMIQALTPKEEFKTSMYN
                     YLEIVTLATICDIVPLIDENRIIVKNGLKSMKEGKNIGLRELIKVCGVESDKIGSSHI
                     GFAIGPRINASGRLGYSYLGVELFTTQSQEEAVEIASILEEKNNERQMIEAKMYHEAE
                     EMLKSNSRYNDDKVLVLAKEGWQHGIIGIVASKLTEKYYKPTILLGIENGEATGSARS
                     IKGFNIFEALIKCKDLMTKFGGHEQAAGLSLDSDNVEILANEINKFADYNLTEDDMIE
                     NVNVEFELQENVINLNLVEELHKLEPFGLNNPNPRFIVRNYILKDLKVIGKNQQHLKL
                     SIEKEKSYECIGFNMSHLKSMYKVGDKVDVLFQLDENNYMGNRKVQFLLKDIRLARPK
                     SASNDKLSLKLMSKIIPKDTQSLYNISVSDFELFDGNTDINIFDYFEKD"
BASE COUNT        10942 a         2598 c         4634 g         9053 t
ORIGIN      
        1 atattttaaa ataaaaacac accccataaa tacaaagtaa tttgcattta agaggtatgt
       61 atttatcatc aaattattgg tttgttttgg aatttctaat cttcaaccat aatatcttta
      121 aatatgaaat catgcattgc atctagttca tattcctcaa aaggtattac atagcctgca
      181 ttacctattt ttctaccatt ttcagtagaa agaggaaact ccatagattt caaatttaaa
      241 tttcctatac ttaaaagttc ttttcctaaa gatattattt cattaggatt catattagtt
      301 tttacatacg gaagtattgt gttaactaga ttaggatatt tagtgactgg taaatcctta
      361 acaccattta ttaaactctg tataacactt ctttgtcttc tatctctttc atctgtacta
      421 tcattttttc ttatacgaga aaatgcaagt gtctgatatc catttaattt ttgctcacca
      481 gaatttctta tatatttaag agaaccttta ttgtcatttt tattccattt ataagtttca
      541 ggaataaatt tatttaattc tcttatttca ctcttatcaa cagtaacagt aactccacct
      601 aaagcatcaa ctatatccat aaaagagaaa aagtcaacta cggcgtagtt ttgtatatca
      661 agttggaagt tttcttctat agtttctatt aataagttag cttgtccata tacgtaggca
      721 tgtgttaatt tttgttctcc acgtcctggt atattaacat aagtatctct atttaatgaa
      781 gttagcttaa gacttttatt tttattatca atagtaagta tcatcatggc atcagaacga
      841 gaaacttctt ctcctggtct tgcatctgta cctaacaata atatgtttgt gatattttct
      901 tcgtttttat agtcattact actaagcgcc tctgtattta tactatcatc ttgtatagtt
      961 tttaatttag taaaaacata tccatatata ctaacaggaa atgctataac taaaagggct
     1021 agaaatataa cgaacttttt gagtcctgac aataatatcc cctactttct aaatttttta
     1081 attacacata gttaattata ttaaaaattt gttacataag caataaaaaa cacaataaat
     1141 tagcataagt ttgtcataat ataattattt ttatagcatt ttatagaaga aagtgtgtag
     1201 ttattattga ttttattgga atataattgt atgaagactt aaatttttat aaactgatgt
     1261 gtatattgaa aaaaatattt tcctgaatgt agttatagca tcttatgaaa ggtgcaaatt
     1321 gtaatttaaa atttttagtg tataataaga attgaaaatt caaaccgata aaaattaata
     1381 aaaaataatt ataaattaat aatttggggg aggaaatgta tgataaaatt aatagcaaca
     1441 gatttagatg gaactttgtt agatgaaaag agcgaaataa atccagagtt ttataaagta
     1501 tttaaaaaat taagagagag agggataatg ttttctgctg ctagtggcag gcagtatcaa
     1561 aatcttataa aaaaatttga agatataaaa gatgatatga tgtttatatc agaaaatgga
     1621 acactagtag tatataaagg aaaagaaata ttatcaaatc ctttaaataa agaattagta
     1681 aatgaaatca tagagacaac aagaagtata aaagggaaaa aaattgtaat gagtgggaaa
     1741 aaatacgctt atatagaaag caaagatgaa gcgtttatac aagaggtcag tacttattat
     1801 gcaaagttta aaattgtaga agatttgacg aaagtagagg gagacatatt gaagatagct
     1861 gtatttgact tcaaaggtgc agaacataat aataatatct actttgaaaa gttttctgat
     1921 agagctcaag tatgtatttc tggagtggag tggcttgatt taactgcaaa aggtgcaaat
     1981 aaagggagtg ccataaaaaa agtacaaaaa atgttagata taaaatatga agaaactatg
     2041 gtatttggtg accagttaaa tgatgtggaa atgatgaaaa gtgcctatca tagttatgcc
     2101 atggaaaatg caaatgaaca tttgaaacaa attgctagat ttagagcaaa aagaaatact
     2161 gaaaatggcg ttgtagataa gataaaagag gttataaaaa ttggatagtc atatgtatag
     2221 atgaaaaaga ttattttgaa gaaatcaaaa aaatatcaat gtaattttga taaataagtt
     2281 tatttatgcg aaaattacat tgatatttaa taaacatcaa taatagatat ttattttttt
     2341 atgatattca gtattttgtt ttctatttga gttgcatttt cagcagaaat taaataaaca
     2401 tatctacctg ccttctcaat tatagtatta tttgctattt cagaattgat tttttctcca
     2461 tgtccttctc caaaaggtgc ttgtatcata tcttttttat aattttctag agttttatag
     2521 attttatcta cctcgtcagt ttttataacg ataatatctt ctagagataa tttttcagaa
     2581 gaattaatta caaaaccttg aattatgtct tcctctacat cattaaaata gttaaaatcc
     2641 tttgcatcaa agatttttga ttcctttata agtaaatcag aagattttaa tgcttttttt
     2701 atttttgatg tagaaacgtt tttgtaatca ccacttgaag aacaactaac cattcctaat
     2761 gataagatta tcgaaaaaaa caatattaaa atttttttca tgataaacct ccaagttttt
     2821 taaactctgt cttaaattag tttactacat aattatataa aaataaatgt atttaatgtt
     2881 acagttttga ttaataatgt aatattttga taataatacc tattgagagg tgattagaaa
     2941 tgagaatacc aaaacatgta ggaattatac cggatggaaa tagaaggtgg gcaagtcaaa
     3001 atggtatgac aaaagataaa ggatatgaaa gtggaataga tcctggacta gagtcttata
     3061 gattatgtag agatttaggc tgtgaagagg tgacttttta tggatttaca actgataata
     3121 caagacgtcc atcagtgcag acaaaagctt ttacaaaagc atgtattgat gctgcaaata
     3181 tattaataga tgaaggtgct gctttattag ttgtaggaaa tacaaaatcg ccaatgtttc
     3241 cagaagaatt attaccatat actaatagaa ttaaagccaa agaaggtcaa ataaaagcaa
     3301 atcttctggt aaattatggt tggtattggg acttaaataa tatatcagga gtagaaaatg
     3361 taaataaaaa taatatacag gattacatac agtcatttga tgtatccaga ctagacttga
     3421 taatcagatg gggtggaaga aggagattaa gtggtttcct tcctgtacaa tcaatttatg
     3481 cagattttta tgttatagat aattattggc cagattttaa gagtgacgat ataaaggacg
     3541 catttagttg gtatgataca caagatgtca ctttaggtgg ataattaaaa atattctgat
     3601 ttaaggttgt agagtgtttt aaattattga aaaaaaaact tcttgatggt aaaataacac
     3661 taataatatg taaaagtgta aaaatttata ccaagaaggg tgaagaaatg tcaaaaggca
     3721 ataataacaa taattcaaga aacaaatcta aaaagaccag tcatctgaat agaaaaaagc
     3781 gaaaattaaa taagaaaaag ttagcagtgt tgatttgctt tacagtttta tttttattta
     3841 ttgcatttaa ggctactcaa ggtgtagtag ctttggtaaa aagtatggat aaaagtaata
     3901 aaactaatca acaacaaaat gttaactcag aacaatttga ccttggtaat gaagaggaaa
     3961 ataaaaagaa aaaatatact gtatttatag acccaggtca tggaggcaat gataaaggaa
     4021 ctgaaagcaa gaccagtaat agatatgaaa aagacctaaa tctacaaatt gcaaaaaaat
     4081 tagcaaataa attatctaaa caaaaggata ttcaagtagt agtatccaga actgatgata
     4141 catatatatc attaaaggat agagctatct tagcaaataa tagttctgct gatgtgcttg
     4201 tttctattca tttaaatgca gagaaaaatg gtaatacagc aacaggtata gaaacttggt
     4261 ataggaataa agctacagat ggttcaaaag aacttgcaca gacagtacaa agtactatag
     4321 tatcctatgt taaagttaga gataggggaa tagttgaaaa taactttgaa gtacttagag
     4381 aatcaaatat gcctgctata ttaatagagt gtggtttttt aacaacacct tcagaggaac
     4441 aaaaaataat aaatgaaaaa tatcaagacc aacttgcgga aggaattgtt caaggtgtac
     4501 tttcatacct agattctaaa gggaataagt aagttaactt tactttaatg tgaggataaa
     4561 tttaaaagtt taactgtgta aattgtatta actttatttc gtaatgttgc aaacattgtt
     4621 tgaatttaga aactatgaaa ttagaaatta ttttatctat atctaatttc atagtttttt
     4681 ttgcaaaaaa tttatagtta taaaatttta aaaaataaat ttagtataat gtaatgtagt
     4741 tatttatatg tgattttaac ttgcattgat ttgctatcaa ttgtgcttta taattagtat
     4801 atataaagtt taactgtaag ataatttaaa aaatagggga atttatatga ataaaaatag
     4861 catgacaaga atttacagtt taaataaaat aatttttatt ataattggaa ttttaggaat
     4921 agttttactc acaagacata ttgaggtaga tattaaagtt ttaattttaa ttttattaac
     4981 cttgtttagt ataaatgtat ttatttcata tcttaaaatt aagttgtatg aggataaaat
     5041 taatttagga ttgataaata attcagataa gtttataaat attactaaag atgagaactt
     5101 cctaaaacat gttcaaaatt atattatcag taatgaaaaa gaaaactgtg taatggcttg
     5161 ttttgattta tgtagactca aacttataaa tgatatatat ggttatgagc taggggatga
     5221 agtgttaaat aatattttaa gtaacttaaa aaattatttt ggaaaagaag ctatttatgg
     5281 aaagttaaaa agtgatgttt ttgtattaat aataaagctt gaaaataggg aacagaagat
     5341 tccttattta gtaaatctta ttaagaataa aattgtgata ctaagtcaga ttgacagttt
     5401 taaaatggat ataaatattg aggcttctat tggaatttat aaatttaaca ataatgaaat
     5461 tgatataaaa aaagctatag ataatgctga tatggcaagg cttaaatcaa aaggactaaa
     5521 gcacatagag tatgtagaat ttgataatgc tatggaagaa gaaatacaat caattatgaa
     5581 aatagagaga gatttattct tggctattaa aaataaagag tttgtaattc actatcaacc
     5641 taaagttgat tcaagtacag gtaacattat aggttcagag gcattgataa gatggttgca
     5701 cccatcatta ggcatggttg gaccaaataa gtttatacct atagctgaaa aaaatggtct
     5761 gataaacaat attggtagat ggctaattca agaagttttt attaccatta acaagtggat
     5821 ttcagaaggt ataaatgtct taccagtctc aataaatctt tctagagtag aactatataa
     5881 aaatgattta gttgattttt ttaaactgat gtttaataca tataatattc caaaagaact
     5941 tatagaatta gaaattacag aaacgacagc actaagagat gtaaaattta taagtgaaag
     6001 gctttatgag attaaatctt taggtatgaa tatatcatta gatgattttg gagtaggtaa
     6061 ttcaaatttt atcaatctta aggggatacc actagatatt ataaaaattg acaggtcact
     6121 tatattggat atagttacaa atacaaaaac aaagtttatg gtgaaagcaa ttgttaacct
     6181 ttcacatgat ttaaacgtaa ctgttatatg tgaaggggta gaggatatgc atcaggtcaa
     6241 agtcttaagt gaactgggat gtaatttcat acaaggttat gtatttttta aacctttgga
     6301 tgagatgaat tataaaaaac ttcttactga cggaagttat ataaatctga aagatactct
     6361 tttagataaa tgcatgacag ttgaggagga gtaaggatga tttcagaaga tgtagattat
     6421 tcaataagaa tcataagagc tatatttaat aatggagaag ataaaccaat agatgctaaa
     6481 atgatattaa aaagtgaaga tataccaata ctaacactat acagactgct tagaaagctt
     6541 tgtgcaaaca atattcttac ttttaacaaa gaagatggca agacatacat gttaaatgtg
     6601 tcattggata caataagtct aagaactatt gtagaagtaa ttgatggaaa aatatatata
     6661 aataaatgta cgagatatgt atctaattgc agtaattgct taaaaggttg taaagtaaat
     6721 cgtgaattgc tagaaataga aaaagaaatt tataaacttt tatcgagaaa atctataaga
     6781 caaatactag aataaaaaat tttcagctat gataataatt ggaggttttc ggttatggat
     6841 gatatatctc aagataattt tctactgtct aaagagtatg agaatagctt agatgtagat
     6901 actaaaaaag caagtggaat atattatact cctaaaataa ttgtagatta tattgtaaaa
     6961 aaaactttaa aaaatcacga tataattaaa aatccatatc cacgaatact tgatatttcc
     7021 tgtgggtgtg gaaattttct tttagaagta tatgatattt tatatgactt atttgaggaa
     7081 aatatatatg aattgaaaaa gaagtatgat gaaaattatt ggactgttga taacatacac
     7141 cgtcatatat taaactattg tatatatggt gctgatattg atgaaaaagc tataagtata
     7201 ttaaaagata gcttaacaaa taaaaaggta gtaaatgatt tagatgaatc tgatataaag
     7261 attaatctat tttgttgtga tagtcttaaa aagaaatggc gatataagtt tgattacata
     7321 gtgggtaatc caccctatat tggtcataaa aaacttgaaa agaaatataa aaaatttctc
     7381 ctagaaaaat atagtgaagt ttataaggat aaagctgact tatatttttg tttttataaa
     7441 aagattatag atattttaaa gcaaggaggg attggcagtg ttataactcc aagatatttt
     7501 ttagaaagtc tatcaggtaa agatttaaga gaatatatta aatcaaatgt caatgttcaa
     7561 gaaatagtgg attttttagg tgcaaatata tttaaaaata taggggtatc tagttgtatt
     7621 ctaacttttg ataagaaaaa aacaaaagag acatatatag atgtatttaa aatcaaaaat
     7681 gaggatatat gtataaataa atttgaaaca ttggaagaac ttctaaagag tagcaaattt
     7741 gaacatttta atataaatca gaggttgcta tctgacgaat ggatacttgt aaataaagat
     7801 gatgaaactt tttataataa gatacaagaa aaatgtaaat atagcttgga agatatagca
     7861 ataagttttc aaggaataat tacagggtgt gataaagcgt ttattttaag taaagatgat
     7921 gtaaagttga accttgtaga tgataaattt ttaaaatgtt ggataaaaag taagaatata
     7981 aataaatata tagtagataa aagtgaatat aggctaatat actcaaatga tatagataat
     8041 gagaatacaa ataaaaggat actagatgaa attatagggc tatataaaac aaaattagaa
     8101 aacagaagag aatgtaaaag tggaataagg aaatggtatg aacttcaatg gggtagagaa
     8161 aagcttttct ttgaaagaaa aaagataatg tatccttata aatcaaatga aaatagattt
     8221 gctatagatt atgataacaa tttttcaagt gcagatgtat attctttttt tataaaagaa
     8281 gagtatttag ataaattttc atatgaatat ctagttggaa ttttaaattc cagtgtttat
     8341 gataaatact ttaaaataac tgctaaaaaa atgagtaaaa atatatatga ttactatcct
     8401 aataaggtta tgaagataag gatttttagg gataataatt atgaagagat agagaattta
     8461 tcaaagcaaa taatatcaat tttattaaat aagtctatag ataaaggaaa agtagaaaaa
     8521 ttgcagataa aaatggataa cttgataatg gattctttag gtatataagc agttgtaagt
     8581 attaaaaaat tataatcttt taattgttta tttaaattta gaaataaaaa gttagccata
     8641 taaaaaatat taaaaatata tacttttcaa tatttttttg ataaaataag aatgaatttg
     8701 tgcaatttta tttttctcca ataactaaaa atagtttttt gaattaatat ttgcttaaga
     8761 ataaaaatca gaaacgtttt tgatatagta ggtgataata tgaaaggatt agttttagaa
     8821 ggtggaggaa caaaaggagc ctaccaaata ggtgcatata aagctttaag agagcttggt
     8881 atagagtttc aaggagtaac tggcacatca attggtgctt taaatggagc atatataatt
     8941 caaaatgata ttgaagtaat ggaagatatt tggttaaatt atgactatac tcattttatg
     9001 aatattgata gagaaaccta tgagaggttt aaaaatgtag atttcactac taaaaatctc
     9061 aatgcagtgt ttgagcttat aaataaagct agaaaaaatg aaggtataga tataagccca
     9121 ttaagacaac taatggagac aacattaaat gaagacataa taaggaaatc aaaaaaagac
     9181 tttggacttg ttacggtctg gtgggataaa aaaattaatc catatccatt gtatatcgaa
     9241 gatataccta aaggaagatt gattgattat ttaattgcta gttccagttt gcctattttt
     9301 caaacgaaca tgatggatga taagttgtat cttgatggaa tgttttgtga taacatgcca
     9361 ataggactct tggaagacaa aggttatgaa gatgttgttg taattaggtt gattgatgat
     9421 tttattggga aaatgaatct aaatcgacat caaaatataa atataaaaac tataatacca
     9481 tcagaatatc taggaggttc tcttaataaa gatagagata atgtagaaaa aaacatccga
     9541 ctaggttact tagatactat gaaagcatat gaaagatgca ctggaattaa gtattatttt
     9601 gatgtagagt ataaatataa tgaagaatat tgttttgata aatttagaag tatgagtaat
     9661 gaaacaatac aatatatctg taatttattg aatataaaaa gagatgcttc acttagaact
     9721 gttatggaaa gtgtaatacc aaagcttggt gaggtgcttg gattgccaaa ggatttttca
     9781 tataaagatt tgttttactg tatatatgaa aagaaacttg aagaaaataa tataaacagg
     9841 ataaaattat atgattttaa caaggttata gatgttgtaa atacaaatat tgattctaat
     9901 agaaatttag cttctaattt tgaagttaga cctaaaatta gtattaaaaa gaacaaaaac
     9961 acaaaattat tgacaaattg tattataaaa gacataaaaa atcaaagtta aataaaaaac
    10021 aaaaaaaaat ttgtaattat actcgaaaaa agtgtatatt agtagtataa tatttttgta
    10081 gcactagtgg gaacaaaaaa tgaccatagg acaaaaaagt accacttagt accattaaag
    10141 taaaaaacag gaaaacaatt ttaaaaaata taaaatgtaa agtttatagg aggatttaaa
    10201 aaaatggaaa aagtagttag tatcaaaaat gcaagtggat tacatgctag accagcagga
    10261 atgtttgtta agaaggcagc tgaatttaaa tcaacagtag aggttgtagc aaaagacaaa
    10321 actgtaaatg caaaatcaat aatgggaata atgagtttag gtttagctca aggagaagaa
    10381 ttaaagttag ttgtaaatgg agaagatgaa gaagtagctt tagctgcaat ggttgaatta
    10441 gttgaaagtg gatttggaga atagatataa taatttttta aaaatttgat atttagaatc
    10501 taatattact taaagtaaag taacagagaa gtattaaaca tatacagacc aacatttgta
    10561 gatatttaat ttctttgtta ctttcatgtt tatttaatca gaagattaag actattgtta
    10621 attgggtata aaaatataaa tattttagaa aatgaaataa gatattttta atcataaatt
    10681 cagagtaaaa atacagtaac aaatagttta taaaatttta taaaaaatta taagtaaata
    10741 aaactataaa aatgtaaatt atttatataa atttataaag tttatgtgct agatgatatt
    10801 ttttaggagg tagggttatg gcttacaaag gaataggtgc ttctcctggt gttgctttag
    10861 ggaaagcgtt agtagttgaa catagtgaat tagtaattga aaaaaaatct atagataatg
    10921 ttgaagctga aattgctaaa ttagaagatg cagttgcagt ttctaaagaa gaattagtaa
    10981 aagttaagga aaaggcttct gaagaattag gagcagagga agcagaaata tttgaagcac
    11041 atttattagt tttagaagac ccagagttaa ttggttctgc aatagataaa attaaaactg
    11101 aaagtgtaaa tgcagagtat gctttaaatg aaataaaaga gatgtttgtt agcatgtttg
    11161 agtctatgga caatgagtac atgaaagaaa gagcggcaga tataaaagat gttacaaata
    11221 gaatacttag acatatatta ggaattaaag ttgttgattt atctgcttta agcgaagaag
    11281 ttgtattaat agcacatgat ttaactccat cagacacagc aactatgaat aaaaaaatgg
    11341 ttttaggatt tttaactgat ataggtggaa gaacttctca tactgctatc atgtctagaa
    11401 ctttagaaat agctgcaatc gttggattaa atgatataac ttcaaaagtt aaggatggag
    11461 attttgtggt tttcaatgga gacacaggtg aagtaatcgt gaatcctgat gaagagacta
    11521 taaataaata tactgaatta aaagctaagt atgaagatga gagaaaagca ttacagctat
    11581 taaaaggaaa accatctgta actttagatg gaaaacatgt ggaattagct ggtaacatag
    11641 gaacacctaa cgatatagaa ggtctaataa agaatgatgc tgaaggtgtt ggtctttata
    11701 gaacagaatt tttatacatg gatagagatt ctttcccaac agaagaaata caatatgaag
    11761 catacaaagc tgtattagaa ggaatggatg gaaagcctat agttataaga acattggata
    11821 taggtggaga taaagaatta agttatttaa gtatggaacc tgaaatgaat ccattcttag
    11881 gttacagagc aataagactt tgcctagata gaaaagatat atttaagact caattgagag
    11941 ctttatatag agctagtgtt catggaagat taagaatcat gttcccaatg atttcatctt
    12001 tagaagagtt gttacaagct aaagaagttg taaaagaagt tttagcagaa cttgattctg
    12061 aaggtgtggc ttatgctaaa gatgtagaaa ttggtatgat gatagaagtt ccatctgctg
    12121 ctgtgatatc tgatgtattg gctaaacacg tagatttctt ctctatagga actaatgatt
    12181 taattcaata cacttgtgca gttgatagaa tgaatcaaaa gataagctat ctttacaatc
    12241 aatttaatcc agctgtttta agacttataa agactgttat agacaatgca cataaagaag
    12301 gaaaatgggc aggtatgtgt ggagaatctg ctggagacca aaaaatgata ccaatacttt
    12361 taggtatggg attagacgag ttctctatgt ctccaatatc aatacttcct gctagaaagt
    12421 taataacttc agttaaagag tcagatatga aaaaattagc tgatgatgta cttaatatgg
    12481 gtactgctga agaaataaaa agctatatag aaaagacttt taatatataa ttaatgttat
    12541 atagttacta aaattcattt ttctaaataa caaaattata ttttacgaat aaaaaaacta
    12601 tagtatatca tatatactat agtttttttg ttatataaat tacgtatcaa aatttgtaaa
    12661 atgaatagat ataattacta aatatgtgat agtattggct ataataggat ataaaaaata
    12721 atattgtaat aaaaagttta tatttagtaa tgaaaattta acgttttact tgaaattatg
    12781 tgttttgtaa tgtattatta aggttctaat aggaacattt tcatcaattt ttctatagtg
    12841 ggggtaggaa ttatgaggta caaaaaaaga aatatagata agtcaagtga actttcattt
    12901 caatctctag caaaatattt tataactttc tatatagtaa ttatattaat tattctttct
    12961 atgttttgta taaatacaat atgggttatg aaaaaagata atcaagattt attatctaca
    13021 acatcaaatc gtattgaaga tagggtagga gaaacaatta agctattgaa ctctttgagt
    13081 aaagataatt acataataga taatagtgtg aatggcatag ataaaagtaa agaactttca
    13141 atgtatagtg atgaatttgg gtatatgatg atatgttttg ttgataagaa tataattgta
    13201 tgggatgaag aaggtggtaa tattagtcta gctggtcgtg attatatgca gaaacttttt
    13261 gcaacaaaaa aatatcaagt tacagatagt tttgcagcag gggctgatgg aaaaacttta
    13321 aactacacta ttgcatcacc tataataaag aatggaaatg tagatggttg tgtatttgca
    13381 gcaatttact ttgatgagat aaaggatata ctcaaacaag gaaaaggtca acagcaacag
    13441 aatttcatat tatttggaag tgaaaatcaa ataatgaatt taacaagtga tgaaactgca
    13501 tatggagaga agtatatgaa gttatcgcag aaaacaaaat atcttggaac caatgcaaaa
    13561 aaagtagaaa aggatatgtt taataataaa tcaggtgatt attgggcttt tgaaaatgga
    13621 aaattatact ttatgtccta taaaccaatt aagaacacaa attggagttt attttgtaaa
    13681 gttaatttct tagatatttt tgcattaaat ttaattccta tgggagctat tctatctgtt
    13741 ataactttag tatttattat tttgatttgt atggagaaaa gcttcatgaa aaaacgaatg
    13801 gaagttattg atatgctttt aggttctgtt caagaattag aaaaacgtat atatcaaaca
    13861 gaaagaccta gtgaagtaga ctttaaagaa attattcagc tcacaagtaa aggtctgact
    13921 gatggattaa ctggtgttgt aacacgtact gtattttttg accaaattta taatcgtgtt
    13981 gaaatggagg aaaatgataa attatctgta atttcatttg ttgatttaga tgatttgaaa
    14041 tgtattaacg acttatatgg acatgaaatt ggggatatgg ttttgaaaaa tgttggatat
    14101 gtacttcgtg aatatgagat aaaatataat ggtttaattg ggcgctatgg tggagatgaa
    14161 tttgttatgt tgttatctgg ttttacatcg agagaagagg tggaggattt attggatgag
    14221 attgctattc gcttacatac tgaagtacta acagatgatg gtatcattca aactcattgt
    14281 agtgttggag ctgtagtatg tagtgtaaaa gacaataaag atatagataa tttattaaaa
    14341 caggctgatg aagctttata ctatgttaaa cgtaatggaa aaggtaatta tcatttagtt
    14401 taatgtatgt ggaaggagtt tatatcatat gaaaaatgta tctaaaaaat tttttgtatg
    14461 tactattatt gtattaataa catttttatg ttttagtaaa tttgctattt atgtttttga
    14521 caaacaagaa aagaaagaaa caaaagtagt agaaaacatg ttaaatgtct acaagaataa
    14581 cattatttta aatctgactg aaaaatttaa ttatagcaaa gacttggcat cagttttagt
    14641 ttcaaacaat tattctatag ataaattaaa acctattgca gatgaaattc ttaaaaacaa
    14701 ttcttgcata tatatttcag ttgtagaaaa atataatgta gctgcatttt tttctaattc
    14761 tgtcaaaaaa gatattatca atactccaat acaggaactg aattattctt attcattagc
    14821 aaattctatc aaaggattag tcgtagaagg tccttctaaa gaccagtttt caaatggaga
    14881 tgtgttttta attactaatc ctatttataa aaatggagag tactggggag aaattacagt
    14941 agctttagat gccaaaaaag ttgtagattc ttttaactta aataaattaa aagaatctgg
    15001 atttgagtat gaactttggc gtgtacacct agataatggt tcaaaatcta caatagaaat
    15061 ttctactaaa gattttaatt tttccaaatc acatgaaatt aagtttaaac ttccgactgt
    15121 ttggactctt agcattgtgt acaaaaatgg atggaataat aagaatatag attttattgt
    15181 atttgttata ttgttaatta tttccatatt aataggtgtg atgacattta tttacattga
    15241 aaagaagtcg attagtaagg aattaaatca acagagtctt aaaaatttag aaactggtat
    15301 tttgaataga gaaggttttt taattaagtt acaagagcat ctgaatagaa aaaataatga
    15361 aatagtgttg ctgtatatat gtattactaa ttatagtaaa tttgctccag ttatgagctt
    15421 tgaaaaaaga caagattatc ttacacatat agtaaaaaat ctagataatt acataaaaag
    15481 ttctcatttt attgctcgta taagtgagga aggttttgtt ctagctttag aagaaaattg
    15541 tgataaaaat gaattgctta acattgaaaa agggatttcc ttagagcttt tacgaaaaat
    15601 taaagtagaa aataaacaag tatttttaac gattggttat ggctcatcta ggtgtgttga
    15661 tgatagaaaa gatgcagaat ctctgttact ttcagctatt gaagattatg agagaagatt
    15721 tccagcaatg tctcctattc atgattttac taaaaagtgt gaagatatgc ttgatgaaaa
    15781 gaaacagatt gtatttggtg aatatccaga tttagaaata aacaagttgt ctaaagtaat
    15841 atacaaatat tataaaaaag tacagcgtat tatgtataaa gattataata ttgatattgg
    15901 aaatagacga gggtatatgc gtgatgtcga agtattaatt gattataatt ctgagagaat
    15961 gttatactta tttttaatag acatatgcaa gtttggaaaa tttaatgaac tgttttctat
    16021 tgaaatagga gatgttattt taaaagaagt tagtaacaaa ttaaaaacaa ttttttctaa
    16081 ttatctatat cgtattaatg gagatgtatt tttaggcatt tcaatagata aagaatctta
    16141 cttagatact ttacataagg ttaatgaagt atttaatagt tctttaagtg tagaagatgt
    16201 actttttgat gtaaaatgta atattggtat ttgtcaatat ccatatcatg gtgaaaatcc
    16261 atatgacctt ctggaaaagg tacaaattgc attggaatat gcaaaaaagg atgtcaaaag
    16321 taatgttaaa gtttatgata aggaattgac agaagagatt taccatgaaa ataatattat
    16381 taaggattta aaaaaaggtc tggaagagca aacactagaa gtttggtatc agccaatttt
    16441 taatgttagt aaaaactgtt atataaaagc tgaggcacta attagattga gaaataaaga
    16501 gggcggttat tattctgctt atgaagttat tacttatgca gaaaagcatg gacttattaa
    16561 ggatgtaggt ctatatgtac taaaaaaggc ttgtgatgta actataaaaa taaaagataa
    16621 aaatatagat tttgaacaaa tgcaattaaa tttatctgtt cagcagattg tacataagca
    16681 gtgtgcaaat gacatattat catgtattga gaattgtggt ttagataaaa agtatatttg
    16741 tgttgaagtt acagaaacag tacttatgga aacttttgaa attgtaaatg ttactcttga
    16801 taaactaaaa aaagaaggca ttagcatagc tttagatgag tttggttctg gatttagtag
    16861 tataaattat ctggcaaatt tacctattga aaatttaaaa atagataagg aattagtaga
    16921 acaaattacc tattctaaaa aacaatttga attattaaaa actatagttc aaattgctaa
    16981 gattaatggc atggatattg tagcagaagg tgttgaaaca aaagaaactc tggatttagt
    17041 tgtaacttca ggtgtgaatt tgattcaagg tcattatttt tcagaacctt taagtgaaga
    17101 taaatttatt gaacttatga ttgaagaata aaggttaata aaataaagat ttaaggtatt
    17161 gaaaaataat taaaagtatt taataataga attagggaag aataagttta taattaaaaa
    17221 taattgatta gtagttaata aaataaatgt gatatccaaa taaatcctaa atctaatttg
    17281 agttttaata tagatagtct aaagtttgat tattaaagat ttaattgtaa gttgttacag
    17341 ttgatgatat tgattagaga ttaaagttta gagtagaaag ttaccttaaa tttaaaaatg
    17401 caagataacc caatctaaaa attataatca ctcaataggt ttaaggattt atttgtttat
    17461 tataaatagg agggtgtctc aaaatgaact ttttagttta tgaggcactc tcttttatat
    17521 agaattaaat atattttatt atttcaacaa tgaaaaagaa gcttatctct attttgagac
    17581 agcctctttg tatactatta ttaatttaat caataacata atttaaaatt atattattag
    17641 ttaagatttc ctgctttatt agtaaataat tctttaatag cattgtttct ggaaactaaa
    17701 tcgttatcag agttgtttgt ttttttcata atcactgaat aacgtttttt atttataaaa
    17761 aatattaaaa tcttatcatc atcaaaattg tattctttat atctgtcaat agactcataa
    17821 cctagattta gaatgttttt gtcttttata ctatctacat ctaaaatata tgatgggtca
    17881 gcctttagta atatattaat taaacatttt tcttcaccag cacagaataa ctccatctta
    17941 cttgatatag atttagattg ctctgaagtt atttttgcag gaataaggtt gtcattatca
    18001 tctcttagaa gataaccatg tgtgtcttgt tgatatacta aagtaacctt tttcttagga
    18061 acactaatca ttttatctaa atgtacataa tgatgatttt caacaaaacc ttcatttaat
    18121 atttcaacaa agttattaat tttacctagg caaaaagatt ctatagattc tttacagaat
    18181 ttatttgtaa atggtattat gtttacgttt tcctcgtctg tattccatgc tatagcataa
    18241 tccaacgaat atacttcttc ctctatatct gcaaaattat attgtactat gtttcctttc
    18301 aaaattactc gacctccatc ttataatata tttaatatac tttataaaac ttatattata
    18361 tttaaataat atcataaatt ggataaatat gatatagtaa ataagaaaaa atttgaaaaa
    18421 aatcactata aatgagtaat aaatataaat agtgtatact aatgggatgg aaaatgtcca
    18481 ctattaactt tattacccca catatataag ataaaattat taatataaaa tataatctat
    18541 cgacaatgaa tagactttta aataaactta atataaaatt aattaaataa gtgcttaaaa
    18601 acgatttact ttaatgatag aatcatatta aaggaggata attttatgga tataaagaat
    18661 aaaattaaat ctatattttt accagaaaaa aatgactatt tagatgatga agaatttgag
    18721 tatgatatag aaaataatga agaagattta aaggaagata acaaagtaca tttaaatgat
    18781 ttaagcagag taaaatatga ttacagagag ttaaaagaca tagaaaacca aacagaagag
    18841 atgtgtctgc tagcagtaaa gcaggatggt actataattg agtttgtaaa ggacaagact
    18901 tataaagtat gtatggaagc agtaaaacag acatataaat ctcttaagta tataacaaat
    18961 caaaatgagg atatttgtat agaagcagta aaacagaact acagagcttt gtactacata
    19021 aataataaga cagaaaatgt actaattgag gctataaaaa atgcatcaac ttatgatgtc
    19081 atggaagtat ttaaatttgt ggaagagcaa acagaagatg tatgtctagc atttatagaa
    19141 agggcatcta aaaatgatgt tgcagagata ttaaaaggaa taaaagagca aactccagcc
    19201 atatgtttag aagctgtaaa aaaagatgga aaatctttag cttatgtaaa ggaacaaagt
    19261 aatagtatat gtttagaagc tgtaaaagaa aactacagtg cactatcttg tgtaaaagag
    19321 caaactgaag aaatatgtat agaagcagta aaacagaatg actttgcatt gtattatgta
    19381 aatgaacaga cagaaaaaat atgtatggaa gcagtaaaaa gaagttatat ggctctacag
    19441 tatgtaaata agcaaacaga agagatttgt ttggaagcag taagaattga tggaagagca
    19501 ttacagtacg taaaagagca aacagaagaa atttgcttgg agtcagttag acaaaatgga
    19561 aaagtactgc aatatgtaaa aaaacagact gaaaatatat gtatagaagc agtaagaggt
    19621 tcatttgaag aacttgaaat aaaagaaata ttaagttatg taaagatacc aacagaaaga
    19681 atatttgtag aagcagtaaa gcaaaatgga aaaattctta aatatgtaga aaatcagaca
    19741 gaattaatat gtttagaggc agtaagagaa aactataatg ctttagctta tgtaaaggaa
    19801 caaacagaaa agatttgttt agaagcagtt aatcaaagtt atgaagcgct taaatatgtg
    19861 aaagaacaaa cagaagaagt ttgtctaaaa gcagtaaaac aagattatag aatgttgaaa
    19921 tatgtaaata atcaaacaga aaaaatatgt ttagaagcag taaagcaaaa ttatagagct
    19981 ttagaatttg ttgataatca aacagaaaaa gtttgtcttg aagcagtaaa acagaataga
    20041 aaagcactac agtatgtaaa acaaaaacaa agttaaaagg ctaaaatata tggggaatat
    20101 atgcaaataa ataaaatttg atattttgta ataaataaaa tgggtatctc aaaaggaact
    20161 ttttagtttg tgaggtactc tttttatatg aaatgaaata tatttttagc cttttaacaa
    20221 tggggaaaag gtttatttct attttgacat aggcttttta tgcatataaa gcttgataaa
    20281 attgttttat tataattgtt ttgttatata aataataaga agaaaattat ataggtcttt
    20341 ttttgaccag ttttacattt ttaacaacta gttttgtaaa gtatattgaa ttttctcaaa
    20401 aaagaatgta ctctatatat gtaaaatttt tttggaggta aaaatgttta aaagatatgc
    20461 tgaaaaaatg acatctgttt taatttgtaa taatatgata gataataatg aatctaaagt
    20521 ctattcttat ggatttgaaa tattaatagc tttcatagtt aatataacca ccatgctgtt
    20581 tataggtttt ttatttggta aatttacata tgtattattt tttttaatgt gttattgtcc
    20641 aataagacaa ttttcaggag ggtatcatgc agacaattat tttagatgct tattaacctt
    20701 tatttttata attttatcta caatcttaat tatagaaaat ataaatatag acttatttaa
    20761 aaatattatt atgattattg caagtgttag ttgggttgga atttgtgttc tgtgcccaat
    20821 agaacatagg agtaacccta taagtgatag ggaaaaattg gtttataaga aaactgctat
    20881 atttatatct acagtagtgt tattgataac aattttaagt ctttctttga gtatatttgt
    20941 agattatttc acatatagcg catttgccat gttttggatt tttatgatgt tagtattagg
    21001 taagttaaaa gctaaagtat aagtaaaata tgtatgaggg agagttaagt tatgaaaaag
    21061 tttattgtaa gatttatgaa gtttgctagc tcattggcac ttagcactgc tattttgtca
    21121 gctaatagta cctgtccttg gattattcat cagcctaaag tacccaaaga aatcagcaat
    21181 ctaaagaaga caaattaaat acaagcactt atttatacaa aattatcagg aaaaaagaaa
    21241 ttattatatt ttaataagat taaaacaata taaaatcaga tacaaaattt attttattta
    21301 ttttatagga gcctaatacc tatttggtaa tatattagtg cgccaattta gggggatata
    21361 gtgtatttaa tgaaatgact tacggagagt aagtcatttt tgtattgtat gtattttatg
    21421 taaacttatg atataatgaa gggtaaagtt taattaaaat aataaaaaac tttagtatag
    21481 atagtaattt aggaggtagg gtatgagtat gatacataaa ttttctatga atggatataa
    21541 tatcgtttta gatgtgaatg gtggagctgt tcatgtgtta gatgatgttg catacgattt
    21601 gttagatttt tataaagaaa aaagtaaaga agaaatatta gagatattaa aatcaaaata
    21661 tcaagaagaa aaaataaatg aagcatatga agaaatatta aatttggaaa aagaaggtct
    21721 tttatataca gaagatacat atcaatatca tccaagcttt gtacatagag agcctgtagt
    21781 aaaagcttta tgtttaaatg tagctcatga ttgtaactta aaatgtaaat actgttttgc
    21841 agctcaaggt gattttggag gagaaaaaga acttatgagt tttgaagttg gtaaggcagc
    21901 aattgactat ttgattgcta attcaggaag tagaaagaac ctagagatag atttctttgg
    21961 tggagagcct ttgatgaact ttgaagttgt aaaacaactg gttgactatg gaagaagtgt
    22021 agaaaaagat tataataaaa atataagatt tacaataact acaaatggtg tactgttaaa
    22081 tgatgagatt atagattata taaatgaaaa tatgcataat gtagttttaa gtttagatgg
    22141 aagaaaagaa gttaatgata atatgagacc aactttaaat gataaaggaa gttatgatat
    22201 tacattacca cgatttaaga aacttgtgga aaaaagagca aaagataagt attattatat
    22261 aagaggtact tttacaaggg ataatttaga tttttcaaaa gatgttatgc attttgcaga
    22321 tttaggattt aaattaacct ctgtagagcc agtcgttgga gatgagagta atccatatgc
    22381 acttagagaa gaagatttac ctaagatatt tgaagagtac gaaaaatttg ctgtagagta
    22441 tgcagataga caattacaag gagatggatt taaatttttc cattttatga tagacctaaa
    22501 tcaaggtcca tgtgtaataa agagaataac tggatgtgga gctggaaatg agtatctttc
    22561 tgtaactcca aatggagata tctatccatg tcatcaattt gttggaaatg aagaatttaa
    22621 aatggcaaat atatttgatg aagaaatagt acttccagaa aatttaaaaa atatgtttag
    22681 agaagctcat gtatatacta aagaagagtg taaacaatgt tggaataagt tttactgttc
    22741 aggtggatgt catgcaaatg cgataaactt taatagtgac atatcaaaac catatgaatt
    22801 aggctgtgaa atgcaaagaa aacgtacaga gtgctctata atgatacaag ctaagttaat
    22861 gttagaagga gcaaccaact aaatttatat tttaaaggag ttgtttatat atgattagag
    22921 attatttaga agataaacct ttaattgacg aaagtgtatt tgtggcaaaa tcagcagatg
    22981 taattggaaa tgttaaaata ggcaaagatt caagtatatg gtacaatgca gtagttagag
    23041 gtgatgaagg accaataact attggagaaa acactaatat acaagattgt tctatagtgc
    23101 atggagatac agaaactata attggtaaca atgtaacagt tgggcatagg tctatagttc
    23161 atggttgtaa aattagtgat aatgtgttaa ttggtatggg ttcaataata ttggataatg
    23221 ctgaaatagg agaatatact ctgataggag caggtacact aattacatct aataagaagt
    23281 ttccaccagg tgttttgata atgggttctc ctggtaaggt tgttagagag cttacagaag
    23341 aagataaaaa atatattgat gaatcttatg aatggtattt agaggcagca caaaatcaaa
    23401 aatattagtt taaagagtgg ttgtttcaaa acatatttaa atctgttttg aggcaaccac
    23461 taatttattt tattagattt tgttgatatt ttagttttca aaaagtattt gattaaacca
    23521 gatataaaat gtcaaaaata aaaattaact gagttaaata attttaataa aattggtagt
    23581 atatgtgata aaattaaata ttaaagagga tataggatat tagaatatca attggtgaaa
    23641 taatgtcata tacgataaat taaggtgtaa gtttttgggt atacctatct taggagtgaa
    23701 aagatttgag aataagttat agaaatttaa agcgatatag agaaattgga tatgttttaa
    23761 taaagtatgg ttttagtttt attgtagaaa gactaaatat agaaggaatt gcctataaaa
    23821 taccactatt tgacccacca gaagaaataa aaaatatgac aactggtgag agaatgaaaa
    23881 gagttttaga agaactagga cctacatata taaaaatagg acagatattg agtacaagaa
    23941 aagacttgtt agaccaggat ataattgatg aaatatcaaa actcagagat gatgtagaga
    24001 aatttgattc aaatatagct attgatatat ttaaagaaga agtgggttta agtatagaag
    24061 aaatattctt agaatttaaa gaagaaccaa tagcagcagc ttcaattggt caagtttatg
    24121 aaggtgtact taaaacggga gaagaagtaa tcgtaaaaat tcaaagacca aatatagaga
    24181 aaataataaa atctgattta gagattctta gaactattgc aaatacttta aaagatttaa
    24241 aaaaagattt taatttagat ttagtacaga tgattgaaga atttcaaact caattaatga
    24301 gggaacttga ttatacattt gaagctataa atgcaactaa attctctagg atatttaaaa
    24361 atagtgatga agtatatata cctaaagtat attcagaata taatactaag aaaattttag
    24421 taatggagaa agttaatggt acaaagttaa gtgatgtaga aaaaataagg agactaggtt
    24481 ataacacaaa aactattgtt gaaataggcg ttaggtcatt ttttacacag gtattatctc
    24541 atggattttt tcatgcagac ccacatccag gtaatatatt cgtagttgct aaaaataaga
    24601 tagcatatat agattttgga atgattggaa ttattgataa taaaacttta aaccaattaa
    24661 atgaaattgc attagcaggt gtagaaaaga atgttgacaa gataatatat ctattgatag
    24721 aaatggatgc attaaatgga gaggctgata ttaagggact tagacaggat ttactttatc
    24781 taatacatta ttactatgat atttctatag aaaaaataaa tgtaacagat atattaaatg
    24841 aactatttag gttttttaga cagtataaaa tagtcatgcc tgcacaattt gtaactcttg
    24901 caaagacagt tataacatta gaaggaacaa gtagaacttt aaacacagat ttttcttttg
    24961 gttctatggg aaaagagttt atgaaacatc attataagag taaatttaac cctaaaaatg
    25021 ttgttttaag ttcaagacaa aatgtagaag aaatattact ggacataaaa actattccaa
    25081 agcaattaaa agcaatttta aaaaatatag aaagaaataa tataaaaatg caaattgaag
    25141 atgttaaaat gacgaggtta gaaaattgta taatagagct aacatctcaa atatctttga
    25201 gtttagtgtt ggcatctatt atagtaggtt cttctcttat aattgcatct ccaaatatag
    25261 aaaataatat atggataaaa tttactgcaa tagcgggttt ctttatatcc tttataatag
    25321 gcctttgctt agttataaga agtattaggt caaaatacaa aaaagattag ggggaattaa
    25381 atttgaaaaa atggatattg aagtataaag gtaagataga ggaaagtgaa tttagcaaaa
    25441 aacttaatat ttccccggaa atctgccaaa ttttaaaaaa tagagggata tgtactgaaa
    25501 aagattcaga gatatttatg aatccatctt tagattattt aagagaccct tttttaatga
    25561 aagatatgca aaaagctgtt gatagaataa agttagcagt tgagaagaac gaaaaaattt
    25621 ggatttacgg agattatgat gttgatggtg tatcatctac atctatcttg tgcttatatt
    25681 ttaagagtat aggttacaat gttgattact atattcctaa tagattagaa gaagggtatg
    25741 gtataaatga agatgctatt aagcttataa gctctagggg ctgtgacttg attataagtg
    25801 ttgactgtgg aattacatca gtgtctgagg tcaatacagc gaatgatttg ggcatagatg
    25861 ttataattac agaccatcat gaatgtcaaa gtgaaattcc atcagccttt gcagtaataa
    25921 atccaaaaca agaagattgt aattatccat ttgattcact atgtggatgt ggtgtagcat
    25981 ttaaaatgat tcaagccttg actccaaaag aagaatttaa aactagtatg tataattatt
    26041 tagaaatagt aacactagca actatttgtg atattgttcc acttatagat gaaaatagaa
    26101 taattgtaaa aaatggttta aaatcaatga aagaaggcaa aaatattgga ttaagggagc
    26161 ttataaaagt ttgtggtgta gaaagtgata agataggttc ttctcatata ggttttgcaa
    26221 taggtcctag gataaatgct tcaggtcgat tgggatattc ttacttagga gtagagctat
    26281 ttactactca atcacaagaa gaggcagtag aaatagctag tatattggaa gaaaaaaata
    26341 atgaaagaca gatgatagaa gctaagatgt atcatgaagc tgaagaaatg ctaaagtcta
    26401 atagcagata caatgatgac aaggtgttgg ttttagctaa agagggttgg caacatggaa
    26461 taataggaat tgtagcatca aagttaactg aaaaatacta caaaccaaca attcttctag
    26521 gtatagaaaa tggggaggct actggttcag cgaggtcaat aaaaggattt aatatatttg
    26581 aagctctcat aaagtgcaaa gacttgatga ctaagtttgg tggtcatgaa caagcagcag
    26641 ggttatcctt ggattcagac aatgtagaaa ttttagcaaa tgaaataaat aaatttgcag
    26701 attataattt aactgaagat gatatgatag agaatgtaaa tgtcgagttt gaattacaag
    26761 aaaatgtaat aaatttaaat cttgtagaag aattacataa gttagaaccc tttggattaa
    26821 ataatcctaa tcctagattt atagttagaa attatatttt aaaagatttg aaagttatag
    26881 gtaagaatca acaacatctg aaattaagta tagaaaaaga gaaaagctat gaatgtatag
    26941 gatttaatat gagtcattta aaatcaatgt ataaagtagg agacaaggta gatgtattat
    27001 tccaattaga tgaaaataac tatatgggaa ataggaaagt acaattttta cttaaagata
    27061 tacgattggc tcgacctaaa agtgcaagta atgacaaact ttcattgaaa ttaatgtcaa
    27121 aaataatacc aaaagatact caaagcctgt ataatatatc tgtgtctgat tttgaattat
    27181 ttgatggaaa tacagatata aatatatttg attattttga aaaagat
//
LOCUS       DBGJFF010000048        27145 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.048, whole genome shotgun sequence.
ACCESSION   DBGJFF010000048 DBGJFF010000000
VERSION     DBGJFF010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27145
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.048"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(29..883)
                     /locus_tag="ACOIAF_001982"
     CDS             complement(29..883)
                     /locus_tag="ACOIAF_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HGZ2668992.1"
                     /translation="MKWRDYADDVNLFEDFDKTIKPLTDEQRRKNINTLIAYFSKEVP
                     SKIYNLSNDEIKPRDILRGLLNVYPPKEIAPEILNMLHNLLLIECEERELVDVNDIEE
                     VEEGIAIWRGNITNLRADAIVNAANNKLLGCLQPLHLCVDNEIHSCAGPRLREDCDKI
                     IKKQGHLEYTGDAKITRGYCLPAKFVVHTVGPIVSGGQPSKEQEKQLLHCYKSCLNTI
                     KEIDEIKNIVFCGISTGVFGYPKKEAANLAVSRVRLWLKENPEKNLKVVFNVFTEEEE
                     EKYRRIFK"
     gene            complement(1206..2534)
                     /locus_tag="ACOIAF_001983"
     CDS             complement(1206..2534)
                     /locus_tag="ACOIAF_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HGZ2668993.1"
                     /translation="MEKFSVVIAGGGSTYTPEIILMLLDNLDRLPLRAIKLYDNDEER
                     QNKVAKACEILIKEKDPNIEYLATTCPKEAYTDVDFCLAHIRVGKLEMRELDEKIPLK
                     HGVVGQETCGPGGIAYGMRSIEGVLENIDYMEKYSPNCWMLNYSNPASIIAEAVRRLR
                     PNSRVINICDMPIGMEHNIARIAGLKSRKHMDIRYFGLNHFGWYTSIKDKKGNELLPK
                     LVEHIKEYGFINGEEGMKNDKKDSWFETNLFTKEIVKTDLTTIPSSYLKYYLFPDYVV
                     NHSDVNYTRANEVIDGREKEVFGSCALIEKQGHSKGSGLKIGIHAEFIVDLATALAFN
                     TQERMLLIVQNNGAIENIQDDAMVEIPCIVGKDGYEPLSIGKIPTFQKGLIEQQLAVE
                     KLVVDAWIEKSYNKLWQALMLSKTVPSATVAKELLDDLIEANKNYWPKLN"
     gene            complement(2682..3011)
                     /locus_tag="ACOIAF_001984"
     CDS             complement(2682..3011)
                     /locus_tag="ACOIAF_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HGZ2668994.1"
                     /translation="MVPEMEEKVFSIISEAGDAKSDVMMSLKEIKKGDYNKAKHLLNS
                     ASEKIQTASKYHLELLSNAMNSEDSGTDFLVVHSEDHYSNALFAHSLVSELVDIFEMM
                     DPRINKN"
     gene            complement(3035..4324)
                     /locus_tag="ACOIAF_001985"
     CDS             complement(3035..4324)
                     /locus_tag="ACOIAF_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HGZ2668995.1"
                     /translation="MEALQNNLRRFLLPIAQKIEKQRHLQAIKEGMISITPIIIVGSL
                     SMLFMALNNMLPEGSAKTLLSENMDTLLIPNKFTMSLLSIYSAFFIAQALAKKYNLNH
                     VEIGMTAVVAQLVVCGQVVDGVLDTSYLDAQGLFVSILVALLVVDITKFMNDKNLVIR
                     FPKEVPSVVNKSFRNLTPMIVCIMLFTAIAAITKNVSGQPLPAIIMNFLAPAISSVDN
                     VFAVTIILFITQLLWFFGLHGAAITSSIWMPIAATYMAENATLIAAGGDPKYVFTIGF
                     YYGFLQVTGSGITLGLVYLMSRSKCKSFNSMGKVVILPSLFGINEPVIFGTPIVMNPY
                     MFVPFVFGPVLVGALNFMALKVGLVGLPIAEPPGFLPPGVAAFLMTLDWKAIVLVFAS
                     IILMTLIYYPFFKIMEKEELNKNAELATTLDDDSFDF"
     gene            complement(4340..4666)
                     /locus_tag="ACOIAF_001986"
     CDS             complement(4340..4666)
                     /locus_tag="ACOIAF_001986"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGZ2668996.1"
                     /translation="MNILLVCANGASTGVLVEKMKSFCSEHEKLKTKTINIEATSFEN
                     LKSYIEANDTDVVLVAPQIRFKEDDVVEACKNYKIAVGLIDTKHYGRMDAPSVMKSAI
                     ELYKNR"
     gene            complement(4801..5430)
                     /locus_tag="ACOIAF_001987"
     CDS             complement(4801..5430)
                     /locus_tag="ACOIAF_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2668997.1"
                     /translation="MQDKILRDKKANDPWARMTTRNGFSADEIISSFQKAIRRNMVEE
                     ACEFAYELYISSPELEDKLWRRILTISVEDIGMGDPSAVIIINNLNQVRKEYSYADGD
                     RPLFFIHAIRYLCACEKDRSSDLLKNIIIKGFAMGKVPEVMDVALDKHTYRGLEMGRD
                     SFHFLNEASIVIPEKEVDNDYKERYLKILEKYKQEEVIDTAFKFNHWQY"
     gene            complement(5645..8302)
                     /locus_tag="ACOIAF_001988"
     CDS             complement(5645..8302)
                     /locus_tag="ACOIAF_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HGZ2668998.1"
                     /translation="MIYKKDKVMKVLESSYRENEKGITANQLSEKTGIARNNISTYLN
                     QLFNEGMVTKIKGRPVYYIPANLNLNKNELLDTQSELLGIKKGTKVENKIKKDSFETL
                     IGKEHSLRPIIEKCKVAMLYPTNGLHTIIYGETGVGKSMIARYMYDYSIDSGIRKNKA
                     PFVTFNCADYANNAQLLMGHIFGVEQGAYTGAESSRKGLLEIANGGILFLDEIHRLPA
                     EGQEMLFTFIDKGTFKRLGDATKERESKVLIICATTENPTSTLLDTFNRRIPMKIEIP
                     SLRDRTMIERMELVKNFFKEESKSISISIKVHKEIIKSLLLYDCKNNVGQLQNDIKLA
                     VANGYLRYKRDNKEPVYIEKKYFDKSINEVSDDYREKSIKADEVITHGIEYFVFTSLG
                     EEEVINYNDINIINAVNTNLNKSVEMNIADASLFNNVKFKSMCESIRQIIKEDEEIDI
                     SESIFKSMAIYIKSVLDKSSSTNNIDLNQIRRNNRSEFKTALKIVGIIENEFDLFLSI
                     ETVAYITLFIVQAKQDEQNKIVNNINIIVAMHGETTASSMVKAVGDIIGKCSAISFDM
                     KLDKSYDEVIVDFKNLINNIDNKDVLLFTDMGSLNSFDEIIKKEKKCGVRVIPMVTTL
                     TVLEAVQKANMGLPLNDVYNSITNTRKYYFGTNEIQNKENLSKTIIIASHVSEGVDNK
                     TRKILEEKMSRYLDGIDIISVPYKTEKDLSLNITKLKESSNIVAVINEQRINIRGIDY
                     ISKKDIDKDENINKLKNIIKISIGYDDVVEGLKTSLKSSNYNRIFKDIKYVSDELFLV
                     FNIEKKYDKVIGLMMHLAFMVDGLIGNTREIEKLDKEKTLDYHKSLSKIKDIVSQLDK
                     KYNIEINEKECYQILLILEYAEIIEKDYQ"
     gene            complement(8661..9605)
                     /locus_tag="ACOIAF_001989"
     CDS             complement(8661..9605)
                     /locus_tag="ACOIAF_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2668999.1"
                     /translation="MNREKILEIKNLKQYFHLDKSTTVKAVDDISFDIYKGEIFGLVG
                     ESGSGKSTTGKTIIRLHESTGGEVIYKGNCISDKKTYKFIKKDVNKSMQIIFQDSTSS
                     LNPRMTIADIISEPLKIQGICKNKTDRMNKVYEMLKLVGLDRSYANKYPSDFSGGQRQ
                     RIGIARALSVDPEFIIADEPIASLDVSIQAQIVNLFKKLQQEKNLTCLFIAHDLSMVR
                     HISDRIGVMYNGKLVELADSNELYNNPIHPYTKSLLSAIPVPDPRYAKSRNRIEYNSN
                     GYDCSNEKLLSWIEVSDGHFVYSSKSDINKYQQNLKVV"
     gene            complement(9598..10623)
                     /locus_tag="ACOIAF_001990"
     CDS             complement(9598..10623)
                     /locus_tag="ACOIAF_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2669000.1"
                     /translation="MEHLLEVNNLSVSFKVEEGEVQAVRNVSFNLKKGETLAIVGESG
                     CGKSVLCKSLMRILPYNGYIKNGEVLLKSSDLVKKSEKEMEDIRGKNISMIFQDPMTS
                     LNPTISIGKQIAEAVIIHQGISKSEAKKRAIELIELVGIDNPEKRFKQFPHHFSGGMR
                     QRIVIAIALACNPDVLIADEPTTALDVTIQAQIIDLIKDLQHKIGLSIIFITHDLGVV
                     ATIADRIAVMYAGKIVEIGTVEDIFYDPRHPYTWGLLGSLPTLDSQDDYLYNIPGMPP
                     NLLNPPKGDAFAIRNKNALKIDYEKEPPMFKINDTHSAATWLLHPDAPEVDVPVRVNC
                     GRVISNE"
     gene            complement(10698..12266)
                     /locus_tag="ACOIAF_001991"
     CDS             complement(10698..12266)
                     /locus_tag="ACOIAF_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGZ2669001.1"
                     /translation="MKLKKLKVLSLVMILSLMAGCSSGGDKDKKADTPKDGKVLVYGS
                     NDYTSINPALYEHGEINSLIFNGLTAHDENNKVVPCLAKDWKFDEATNTYTFNLRDDV
                     KWHDGEKFTANDVKFTIETIMNPDNASEIASNYEDITKIDVVNDNTIKITLKAPNTAM
                     LDYLTVGVLPKHALEGKDIATDEFNQKPIGTGPFKLEKWDKGQSITLVKNSDYFVKEP
                     GLDKVVFKIVPDDKAKAMQLKSGELDLAQITPKDMSNFEKDEKNFKVNIMKTADYRGI
                     LYNFNSKFFKDKKAKGLPNALSYAIDRKAIVDSVLLGHGVPAYSPLQMGPYNNPDIEK
                     FEYNPEKAKQEIEKLGWKLGSDGIYEKEGTKLAFEITAGESDQVRVDMAKICAQQLKE
                     IGVDAKAVVVTETDWANQDAHLIGWGSPFDPDDHTYKVFGTDKGANYSAYSNPTIDKI
                     LQKARETEDKDEKLKLYKQFQVEMTKDMPYTFIAYIDAIYVGKPNIKGLTPDTVLGHH
                     GVGIFWNIADWTIE"
     gene            complement(12379..13290)
                     /locus_tag="ACOIAF_001992"
     CDS             complement(12379..13290)
                     /locus_tag="ACOIAF_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2669002.1"
                     /translation="MKPQYSDFEIVGENYILVAEDEHIETKKTLSKKIKELPYISIII
                     LSIIVIGSVFSSFIMTHEPTYMDLASSNLAPNKTFLFGTDSMGRDIYSMIWYGGKISL
                     FIGLFSTVISTVIGIVYGSISGSASELVDDAMMRFTEIILSIPSILIIIFVQAILGNS
                     NPVSMSIVIGITSWMNISKIVRTEVRQIRNSEYILAAKSMGGGFFYVLKQHLLPNFVA
                     SIMFMVVTNIGAAIGTESTLSFLGIGLPIEIVSWGSMLSLSEEALLSNRWWIILIPGI
                     FLVTTLVCITNIGNYIRKNNNKKSSNL"
     gene            complement(13274..14260)
                     /locus_tag="ACOIAF_001993"
     CDS             complement(13274..14260)
                     /locus_tag="ACOIAF_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2669003.1"
                     /translation="MLKDILKKLLQFILVMFLLSFIVFYMARLAPGDPLISYYGDGVE
                     RMSTQEKENAMKKLGLDEPIYSQYIKWISNASKGEFGISFKYKQNVTSVINDVYINTI
                     ILGGSGYILTFVLALLLGIFCTLHEDKLIDRIICKLGTITNCIPSFWVALVLILIFSI
                     NLSILPSSGAYSMGEESSILSRISHLILPLTVLILSHLWYYTYMIRNKLLEEIREDYV
                     LLCKAKGLNNRTIVFKHCLRNIMPSYISIMAISIPHILGGTYVVEKVFSYPGLGTLCF
                     ESAKYHDYNMLLVLCLITGALVVFGNMLAQIINNKIDPRMKYDRGDTNEATI"
     gene            14578..15021
                     /locus_tag="ACOIAF_001994"
     CDS             14578..15021
                     /locus_tag="ACOIAF_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HGZ2669004.1"
                     /translation="MILLKSNYDCFRIAMLLKELYSKTMYTVEENFKENGLTHQQIIV
                     IKLIAHNQELTISQLCDEMSLAKGTVSGIISRLEQIGYIEKFKKSNDKRNTYVKFTTT
                     GFEFATNFKIKMQESFDDIFKNCDENELSDLVKNLRNILAKVKER"
     gene            15024..15422
                     /locus_tag="ACOIAF_001995"
     CDS             15024..15422
                     /locus_tag="ACOIAF_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsmA family protein"
                     /protein_id="HGZ2669005.1"
                     /translation="MNYKLILAIVFITSALIFYTIGVFGERKAKILKKKHVIIFWLGF
                     IFDTLGTFTMSNIANSHTFEVKSALSQNLHSITGLLAIVLMLFHASWATFVLYKDDEE
                     KKKFFHKFSIVVWTIWLVPYFIGMFIGMAG"
     gene            complement(15872..16096)
                     /locus_tag="ACOIAF_001996"
     CDS             complement(15872..16096)
                     /locus_tag="ACOIAF_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015759013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HGZ2669006.1"
                     /translation="MREKVEKVLEEKIKPVLQRDGGDVELIDVNENGVVLVRLQGACS
                     GCPGATMTIKAIIENVLVSEVPGVTQVLGV"
     gene            complement(16131..17498)
                     /locus_tag="ACOIAF_001997"
     CDS             complement(16131..17498)
                     /locus_tag="ACOIAF_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HGZ2669007.1"
                     /translation="MKEYIINNVEEIREELIDLSKKIWENPELAFEEKYASSIQKEYL
                     KSKGFKIEEVENLPTGFIASFGEGKPVIGILGEYDALPELSQCVSAERKPLVEGKAGH
                     GCGHNLLGVAGVGAVVSIKKLIEENKFNGTIKYFGCPAEEEGGGKTVMCINGCFDDVD
                     CAFTWHPFDINAPWRGGSLANLSVKFKFKGITAHAAQAPHNGRSALDAVEIMNVGANY
                     LREHVIDSIRMHYVITNGGGRPNVVPGFAESWYFIRGKKAKDAEHVLDRLIKVAKGAA
                     MMTETEMEYKVTDGIYDYIPNQCLTDLVYDNMVFVGCPKNTPEEEEFAKKLCDTLTRE
                     ERLGVATSLQNDKSIVESYIHKEIVDGDKDKGLAGSTDVGDVSYVIPVAQFAMAAWPV
                     GIASHTWQSCSSAGSNIGFSAMINSAKVLACSAYDVFMDTKIIDEAKIEFDKSLDGQK
                     FKPLV"
     gene            complement(17582..19117)
                     /locus_tag="ACOIAF_001998"
     CDS             complement(17582..19117)
                     /locus_tag="ACOIAF_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6179 domain-containing protein"
                     /protein_id="HGZ2669008.1"
                     /translation="MLNNLSNININENNLIKNQYSISLLKECLNCKVIDEREVYNIQQ
                     EVSLILMDLIKKYTNGQSTSVKTEVAEKLLISIWYAIDAYINKLGDIEKRVVELTAKN
                     VKKVYLGGIEILEEELVRLKDFYKLMMSRKLDTNSIAYNDTLMELSSFFKSYIVKFEA
                     QDVPVSIDYPLALDDMDVKGIYYVKNYIESIDIENRFCNLFKKKDREKLFYDYGVTYK
                     IDCDVILVNLFELIINNCIFSTILGNEVTNLEISKYEYEFLEKQFKNMYNNITEDKVI
                     DSEESNIIELIEVDDSFNTEVDSNLDYNDFEEINREIRINKKITLLLMEAIEVLIKRL
                     SIEDEDVIRYIKKYEKIFIESVLSSIKSNTLKSMVTVSKNKNEHLEDYIVDDESKLDD
                     ESFREIFNEILDSTSIIEKIRIINENINAKKDFIDILESECLFGEEYLMLFASLSELE
                     LAILGSVVFYEDIRMREINILEFMLNEKTETTLWKIEYIKFMKRQSEDKIYSIEEHMN
                     KIN"
     gene            complement(19140..19574)
                     /locus_tag="ACOIAF_001999"
     CDS             complement(19140..19574)
                     /locus_tag="ACOIAF_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6323 family protein"
                     /protein_id="HGZ2669009.1"
                     /translation="MISPIILSSINQNLKEIERNELLETNIESGDYGLALSESDVKDI
                     INSRDNTLKGYGRIELDIKVTKQLIENIYTSQYTNVDNYLEAINDMQEIFYYLKNETD
                     DKICDDEIIEILGEFYEKFSGNMDNVRGEADEFAKKFKFGEV"
     gene            complement(19839..20747)
                     /locus_tag="ACOIAF_002000"
     CDS             complement(19839..20747)
                     /locus_tag="ACOIAF_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HGZ2669010.1"
                     /translation="MNKKIELIGNFASDWIRYSDYEYKITEEDELYIVPTEDAVFTMY
                     NPFDVADDIIVDIIRIGQEALQDISKETKEMKLKKEVLEFVKKYGLLGLIYSSVYNRD
                     IVGEERVLMIEKNYITKENILSTDEYVNMFIPFASDEDVYFKKYRRGIDVSKREESPK
                     FFGKRPLILDLVFSKFYTEQLDWIVSFAKDMCKHFNQICVYKNTSNLLTESVTIMSGG
                     FNPQKIGFSISQLDKPIIAWEFDSLKTAIETIYAFAITSEPSSLNKCKYCGKIYIPTN
                     SKSQYCSPSCRNCSNVKKSRSRAKKR"
     gene            21043..21396
                     /locus_tag="ACOIAF_002001"
     CDS             21043..21396
                     /locus_tag="ACOIAF_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HGZ2669011.1"
                     /translation="MDRKRELKQLYKEMKFDTGVFIIKNDITKKIYLGKSNDIKSKFN
                     SLKFQLGAGSCMIKELNNEWKKYGEKAFTFEVLELIKHDDNKTEKDYLEELDILEMVW
                     LEKLKEDNTYEIYSI"
     gene            complement(21615..22556)
                     /locus_tag="ACOIAF_002002"
     CDS             complement(21615..22556)
                     /locus_tag="ACOIAF_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp25"
                     /protein_id="HGZ2669012.1"
                     /translation="MRISKKVLALGISALLLSVSFPTSINALDKIENIQGVDKYETAG
                     LISDKQDFTTAVLINADSTMVDGIAASGLAGVNNAAILLTNKDDIPEATLQRLNRVTK
                     IYVIGGENSISKDVEKMLLMRRMQVIRIDGVDRVDTSYKIAGEIEKIKNSDKMFLVNG
                     FKDEADAVSVASVAYRDGAPIILTKDIPSAEEDTDLDPWFGVSPVPVYAIGGESTLSD
                     YIVSRYRATRIGGVDRYQTNKNVIEKFYNGAKEFYITSGDDLVYALVASPLAKNAPVV
                     LVSNKSDKSILSGASKVTAIGISDKSIIEQCLDAVKK"
     gene            complement(22657..23892)
                     /locus_tag="ACOIAF_002003"
     CDS             complement(22657..23892)
                     /locus_tag="ACOIAF_002003"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HGZ2669013.1"
                     /translation="MKILHIITQKPNSTGSGIYLSGMIKGFEKIGHKQAVIAGIDVND
                     DVNCFPSEVSFYPVKYNCGELNFPVVGMSDSMPYESTRYKDLNIDMINRLKYQFKVNI
                     DKAMNDFKPELIICHHLYLLTAFVREMVKDIKVMSICHGTCLRQLNTIDLEKEYIIAN
                     IRKLDLIFALHENQKYDIIKTFGVSESKVVVIGSGYNDDIFYNKNYKIKDDKIKIVFA
                     GKICKSKGLIPFIKAISKLKYSKDLIEVNFAGTGSDIESYNEIVKLASKSPFKMNFLG
                     KLEQRDLAELFNRSQIFVLPSFYEGLPVVVLEALSCGTDVITTDILGVKEWIGSEINN
                     SGKIEYVSLPFMEKEGIPKDEELYDFENNLYNAIDSKIQSLLNNSNKKTSVDMSKKTW
                     DGLAYRINEVILMDELCLV"
     gene            complement(23936..24790)
                     /locus_tag="ACOIAF_002004"
     CDS             complement(23936..24790)
                     /locus_tag="ACOIAF_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669014.1"
                     /translation="MALNIRNYSLLCNQGTAKFNEDVVGINPFGAWVLDGATGLNGKN
                     LVSTESDARWYVQWWNRYLYKNINKEESLKSIINTGIIEVKKEYELRLNGFKVEKLDL
                     PSSSIVVVKFHENKVEYFLLGDCALFIKNGDSKVIKDRSICKFDNTVFDEMSKLSNLE
                     EMAFDEIRTSVMDTIVSNRLKKNTKDGYWILDFDEEAVENSIYGYIEIKKDFQLMLTS
                     DGFTSACDRYNLIKEEELIRIAEKFGIGYIHNKVRDFEDNDYKAVKVPRFKVKDDSSC
                     LYLDIYND"
     gene            complement(24918..26093)
                     /locus_tag="ACOIAF_002005"
     CDS             complement(24918..26093)
                     /locus_tag="ACOIAF_002005"
                     /EC_number="2.1.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolecarboxamide
                     formyltransferase"
                     /protein_id="HGZ2669015.1"
                     /translation="MARELELKYGCNPNQKPSKIYMKNGELPIEVLNGKPGYINFLDA
                     FNSWQLVKELKEATGLPAATSFKHVSPAGAAVGVPLSDTLKQIYFVDDLELSPLACAY
                     AMARGADRMSSYGDFIALSDVCDKETATIIAREVSDGIIAPGYTDEALEILKGKRKGN
                     YNIVKIDENYTPEPIETKDVYGITFEQGRNEILINEDLLKDIPTDNKIFTDSAKRDLI
                     IALITLKYTQSNSVCYAKDGQVIGVGAGQQSRIHCTRLAGNKADTWYLRQHPKVLNLK
                     FKKDIGRPDRDNTIDVYLSDDYMDVLADGIWQNFFEEKPEPLTGEGKRAWLKTLTGVA
                     LGSDAFFPFGDNIERAKRSGVSFIAQPGGSIRDDNVILTCNKYNIVMAFTKNRLFHH"
     gene            complement(26192..27076)
                     /locus_tag="ACOIAF_002006"
     CDS             complement(26192..27076)
                     /locus_tag="ACOIAF_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HGZ2669016.1"
                     /translation="MKFVTFCSSNEEKIGVFNSETNSIYEINSLGLSKLYTDMNDFIE
                     NVSTGDLEKIKNNSFENAKCYKLEEVKLCSPIVRPKKDIICLGLNYKDHVNEIPDGVI
                     KNVVMPDYPIYFSKRADKIIGVDDKISLHGDLVEKLDYESELAVIIGKEGINISKEDA
                     YEYIFGYTIVNDISERALQDKHVQWFRGKSLDTHTSMGPCIVHKEEFEHPLKLDISSV
                     VNGEVRQNSNTEYFIFDIPTIINDLSRGMTLKPGDIISTGTPAGVAMGMNPQVYLKHG
                     DIVECKVEGIGVLKNIVD"
BASE COUNT         8653 a         4859 c         2782 g        10851 t
ORIGIN      
        1 agtattttta ggattccgtt acataatatt atttaaatat tcttctatat ttctcttctt
       61 cttcttcagt aaacacatta aatacaactt ttaaattttt ttcaggattt tcttttagcc
      121 atagtctaac tctacttaca gcaagattag ctgcctcttt ttttggataa ccaaacacac
      181 ctgtagaaat tccacaaaaa acaatatttt ttatttcatc aatttctttt attgtattta
      241 aacatgattt ataacaatga agcagttgct tttcttgctc tttacttggc tgaccaccag
      301 atacaatagg tccaacagta tgtactacaa attttgcagg taaacaataa cctctagtta
      361 tcttagcatc tccagtatat tctaaatgcc cttgtttttt tattattttg tcacaatctt
      421 ctctaagtct aggacctgcg catgaatgta tctcattatc cacgcataga tgtaatggtt
      481 gtaaacaccc taataatttg ttatttgcag catttactat tgcatctgct cttaaattcg
      541 ttatattacc tctccatatg gcaatccctt cctctacttc ttctatatca tttacatcta
      601 ctaactctct ttcttcacat tcaatcaaca ataaattatg taacatatta agtatctctg
      661 gagcaatttc ttttggtgga tatacattca aaagtcctct aagtatatct ctaggcttaa
      721 tttcatcatt tgataaattg tatattttag aaggaacttc ttttgaaaaa tatgcaatta
      781 aagtgtttat attttttctt ctttgttcgt cagttagagg cttgatagtt ttatcaaaat
      841 cctcaaataa atttacatca tcagcataat ctctccactt catagccatc tctccattct
      901 tgtattttag acttaaaaat aattatgtat ttatgtacat atttatttat ttctataaac
      961 acctacataa atcctgttaa aagtgtaaat tttttattat gcaacttctc tggttatttc
     1021 tattgattga tagtaaatta ttgtacctaa atttaatcca actaacaagt aactaaacct
     1081 actttcctaa ctgcaaaata cactatgttt gccacttgta tctcatttgt aaataaataa
     1141 agtccctaca aatacattaa ctaggctatg tactaaactg taggaacttt aaaaatatat
     1201 tattcttaat taagttttgg ccaataattt ttatttgctt ctattaaatc atctagtaat
     1261 tcttttgcaa ctgttgcact aggaactgtc ttagatagca taagtgcttg ccacaattta
     1321 ttataagact tctcaatcca agcatcaact actaattttt caactgctaa ttgttgttct
     1381 attaaacctt tttggaatgt tggtattttc cctattgata atggttcata cccgtctttt
     1441 ccaactatac atggaatttc aaccatagca tcatcttgta tattttctat agctccatta
     1501 ttttgaacta tcaataacat acgctcctgt gtattaaaag ccagagctgt agctaaatcc
     1561 actataaact ctgcatgtat tcctatctta agcccactgc ccttagaatg tccttgcttt
     1621 tctatcaatg cacatgaacc aaaaacttct ttttctcttc catcaataac ttcattggca
     1681 cgagtataat ttacatcaga atgatttaca acataatctg gaaatagata gtactttaag
     1741 taactacttg gtattgtagt taaatctgtt tttactattt ctttagtaaa taaattagtt
     1801 tcaaaccaac tatctttctt gtcatttttc attccttctt caccatttat aaaaccatat
     1861 tcttttatat gttctactaa ttttggtaaa agttcatttc cttttttatc tttaattgaa
     1921 gtataccaac caaagtgatt taatccaaag tatcttatat ccatatgttt acgagatttg
     1981 agtcctgcaa tccttgctat attatgttcc atacctattg gcatatcaca aatatttatt
     2041 accctagaat taggtctcaa ccttctaaca gcttccgcta ttattgatgc tggattagaa
     2101 taatttaaca tccaacaatt tggagaatac ttttccatat aatctatgtt ttcaagaaca
     2161 ccttctatag acctcattcc ataagctatt ccacctggtc cacaagtttc ttgaccaact
     2221 accccatgtt ttagaggtat cttttcatct agttctctca tttcaagttt tccaactctt
     2281 atatgagcta aacaaaaatc tacatctgta taagcctctt tagggcatgt tgttgctaaa
     2341 tactctatat tagggtcctt ttcctttatt agtatttcac aagctttagc taccttattt
     2401 tgtctttctt catcattatc atataatttg attgctctta atggtaacct atctaagtta
     2461 tctagcaaca ttaatatgat ttctggggta tatgtacttc ctcctccagc tattactact
     2521 gaaaattttt ccataataat atctcctttt ctatttaaaa atttactgta tttataaata
     2581 tatttataaa tactagtact ttaaagtaaa agggagttca tttattaaat ctattaattt
     2641 aataaactat agccaaactt caagcactat ttacaagaaa tttaattctt attaattctt
     2701 gggtccatca tctcaaatat atctacaagt tcagatacta aagaatgagc aaataatgca
     2761 tttgaataat ggtcttcaga gtgcacaact aaaaaatcag tcccactatc ttcactattc
     2821 atagcatttg atagtaattc taaatgatac tttgatgctg tttgtatttt ctctgaggct
     2881 gaatttaaaa ggtgttttgc tttattgtag tcaccttttt ttatttcctt aagagacatc
     2941 attacatcac ttttagcatc tcctgcttct gatattatac taaatacttt ttcttccatt
     3001 tcaggtacca tatccttctc cttctaatga cttgttaaaa atcaaagcta tcatcatcta
     3061 atgttgtagc taattctgca tttttattta attcttcctt ctccattatc ttaaagaatg
     3121 gataatatat caaggtcata agtattatac ttgcaaatac taatacgatt gccttccaat
     3181 ctaatgtcat caaaaatgct gcaacccctg gaggtaaaaa acctggtggt tctgcaatag
     3241 gtaatcctac taatcctact tttaatgcca taaaattaag tgctccaacc aatacaggtc
     3301 caaatacaaa tggtacaaac atatatgggt tcataactat tggtgttcca aatattactg
     3361 gctcatttat tccaaatagt gatggtaaaa tcacaacttt tcccatacta ttaaaacttt
     3421 tacacttact tcttgacata aggtacacta atcccaaagt tatccctgaa ccagttactt
     3481 gcaagaaacc atagtaaaat cctattgtaa acacatattt tgggtctcct cctgctgcta
     3541 tcagtgttgc attttctgcc atatatgtag ctgctattgg catccatata cttgaagtta
     3601 ttgctgctcc atgaagccca aagaaccata ataattgagt tataaatagt attatagtta
     3661 ctgcaaaaac attatctaca ctgcttatag ctggtgctaa gaaattcatt ataatagctg
     3721 gtagtggttg acctgagaca tttttagtta ttgctgctat tgctgtaaat aacattatac
     3781 atactatcat tggagttaag tttctaaaac ttttatttac tacactaggt acttcttttg
     3841 gaaatctaat tacgagattt ttatcattca taaacttagt aatatcaact actaaaagtg
     3901 ctactaatat acttacaaat aaaccttgtg catctaaata acttgtatct aatacaccat
     3961 ctactacttg tccacataca acaagttgtg caacaactgc tgtcatacct atttctacat
     4021 gattaagatt atatttttta gctagtgcct gagctataaa aaatgctgaa tatatagata
     4081 ataaactcat agtaaattta ttagggataa gtaatgtatc catattttca gataataatg
     4141 tttttgcact tccttcaggt aacatattgt ttaatgccat aaataacata cttaaagaac
     4201 caacaattat tattggggta atacttatca taccttcttt tatagcttga agatgccttt
     4261 gtttttcaat cttttgtgct attggaagaa gaaatcttct taaattattt tgaagtgctt
     4321 ccatttttat tccccccaat tatctatttt tatataattc gattgccgac ttcataacag
     4381 aaggagcatc catacgacca taatgcttag tatctattaa accaactgct attttgtaat
     4441 tcttacaagc ttcaactaca tcatcttcct taaacctaat ttgtggtgct acaagtacta
     4501 catctgtatc attagcttca atgtatgatt ttaaattttc aaatgatgtt gcctctatat
     4561 ttatagtttt agtttttaac ttttcatgct cactacaaaa acttttcatc ttttccacaa
     4621 gtacacctgt tgaagctcca tttgcacata ctaataaaat attcataatt accctcctac
     4681 aatatctaac ctcttttgtt ttctaatttt ttatcagcca attcttactc taattcctat
     4741 cagtttttac tttaattctt attattttat tttaattctt agtaatattt actctaattc
     4801 ttagtactgc caatgattaa acttgaaagc cgtatctata acttcttctt gcttatattt
     4861 ttctaatatt tttaagtatc tttctttata atcattatct acttcttttt ctggtattac
     4921 tatactagct tcatttaaaa agtggaatga atcccttccc atttctaatc ctctatatgt
     4981 atgcttgtca agtgccacgt ccataacttc tggaacttta cccattgcaa atccttttat
     5041 aattatattt tttaataaat cacttgatct atctttctca catgcacaaa gatatcttat
     5101 tgcatgtatg aaaaataaag gtctgtctcc atctgcatat gaatattcct ttcttacttg
     5161 atttaaatta tttatgatta ttacagctga tgggtccccc attcctatat cttcaactga
     5221 tattgtaagt atccttctcc atagcttatc ttctaattct ggtgatgata tatatagttc
     5281 ataagcaaat tcacaagctt cttccaccat atttcttctt atagcttttt gaaatgacga
     5341 tattatttcg tctgcactaa atccgtttct tgtagtcatt cttgcccatg ggtcatttgc
     5401 ctttttatct cttaatattt tatcttgcat attatttact cctttttatt taaattttaa
     5461 ttatttatat tactcattta ataatataag caagtattat gccagttttt ttagacactt
     5521 taaaacactg ttatttcaat catttttatc atcaaaactt agatactttg tctaagtata
     5581 tttatatgtg tctaatatta atattatttt agactaaaaa aaattaccaa ataagttttt
     5641 atactcattg gtaatctttt tcgattattt ctgcatattc taatattaat aatatttgat
     5701 agcactcttt ttcattaatt tctatattat attttttatc taattgagat actatatctt
     5761 ttattttgct taggctcttg tgataatcaa gtgttttttc tttatcaagc ttttcaattt
     5821 ctctagtatt tccaatcaac ccatctacca tgaaagctaa atgcatcata agtccaatta
     5881 ctttatcgta tttcttttct atattaaata ctaaaaacaa ttcatcagac acatatttta
     5941 tatctttaaa tatcctatta taattagagc tttttaagga tgtctttaaa ccttcaacta
     6001 catcatcata acctatactt atctttataa tattttttaa tttattgata ttttcatcct
     6061 tatctatatc ttttttgcta atatagtcaa ttcctctaat attaattctt tgttcattga
     6121 ttactgcaac aatattacta ctttctttaa gtttcgtaat atttaaactt aaatcttttt
     6181 ctgttttata tgggacagat ataatatcta ttccatctaa gtacctactc atcttttctt
     6241 ctaatatttt tcttgtttta ttatctacac cttctgaaac atgtgatgct ataataattg
     6301 ttttagacag attttcttta ttttgaattt cattagttcc aaaatagtac tttctcgtgt
     6361 tagtgataga gttgtatacg tcatttaaag gtaatcccat attggccttc tgaactgctt
     6421 ctaacacagt taatgtcgtc accataggaa ttactcttac tccacacttt ttttctttct
     6481 ttataatttc atcaaaagag tttaatgaac ccatatctgt aaatagtaat acatctttat
     6541 tgtctatatt attaattaaa tttttaaaat ctacaattac ttcatcatag cttttatcta
     6601 acttcatatc gaaagaaata gcactgcact ttccaataat atcaccaact gcttttacca
     6661 tactacttgc tgtagtttct ccatgcatag caactataat attaatgtta ttcactattt
     6721 tattttgttc atcttgttta gcttgtacta tgaataatgt aatatatgct acagtttcta
     6781 tagataaaaa taaatcaaat tcattttcaa ttattccaac tatttttaat gctgttttaa
     6841 attcacttct gttatttctt ctaatttgat tcaaatctat gttgtttgta gagctacttt
     6901 tatccaatac actttttata taaattgcca ttgacttaaa tattgattca cttatatcta
     6961 tttcttcatc ttctttaata atttgtctta ttgattcaca catgctttta aacttaacat
     7021 tattaaatag agaagcatct gctatattca tttctacaga tttattaaga ttagtattta
     7081 ctgcattaat aatatttata tcattgtagt taataacttc ttcttctcct aatgacgtaa
     7141 atacaaaata ttctattcca tgtgttataa cttcatctgc tttaatagac ttctctctat
     7201 aatcatcact cacttcattt atagatttat caaaatattt tttctctata taaactggtt
     7261 ctttattatc tcttttatat cttaggtatc catttgcaac agctaatttt atatcatttt
     7321 gaagttgacc tacattattt ttacaatcat aaagtaatag tgatttaatt atttctttat
     7381 gtacttttat tgaaatagaa atactttttg attcttcctt gaaaaaattt ttgactaatt
     7441 ccattctttc aatcattgtc ctatctctca atgatggtat ttctattttc attggtattc
     7501 ttctattgaa tgtatctaat agtgttgatg tcggattttc tgtagtagca catattatta
     7561 ataccttaga ctctctttct ttcgttgcat ctcctagtct tttaaatgtt cccttatcta
     7621 taaaagtaaa caacatttct tgaccttctg ctggtagtct atgaatttca tctaaaaata
     7681 gtatccctcc atttgcaatt tccaaaagac cttttctgga agattctgca cctgtataag
     7741 ctccttgttc taccccaaaa atatgtccca taagtaattg tgcattatta gcataatcag
     7801 cacaattaaa tgttacaaaa ggagctttat tttttctaat tccactatct atagaataat
     7861 cgtacatata tctagcaatc atactctttc ctactcctgt ttctccatag ataatagtat
     7921 gaagtccatt tgtaggatag agcattgcga ccttgcattt ttctattata ggtcttaaac
     7981 tatgttcttt acctattagt gtttcaaaac tatctttttt tattttgttt tctacttttg
     8041 ttcctttttt tatgccaagc aactcacttt gtgtatcaag caattcattc ttgttaaggt
     8101 taagatttgc agggatataa tacacaggtc ttccttttat cttcgtaacc ataccctcat
     8161 tgaataattg gtttaaatat gtacttatat tgtttcttgc aattcctgtc ttttctgata
     8221 attggttagc tgtaatccct ttttcatttt ccctatatga tgattctaaa actttcatta
     8281 ctttatcttt tttatatatc attacaagct cccctacatt atagtatttt attaactaaa
     8341 acccctatac acactttatt ttttaataaa ctcttattct ttaactaagg ttataattta
     8401 tcaaactttc cttactttaa attatagaaa attattgtag aaaaaataag attattgctc
     8461 attttttcta cattactatt tttaatttta tagtactttc ttaacaacat actttacttg
     8521 tttttaattt tattatattt gaatttatta tatttgaaaa gtagaaataa aaaaccctat
     8581 tttggagtag ttttaaaatt tctccaaaaa tagggtttta gtttgtattt caaataaata
     8641 cctgttcaag tatttttata ctaaacaact tttaaatttt gttggtattt atttatgtct
     8701 gatttagaag aatatacaaa atgtccatct gaaacctcaa tccaacttaa taacttttca
     8761 tttgaacaat catatccatt agaattatac tctattctat ttcttgactt agcatatcta
     8821 gggtcaggaa caggaattgc agaaagtagt gattttgtat atggatgaat aggattatta
     8881 tacaattcat ttgaatctgc caattctact aattttccat tatacataac tcctatacgg
     8941 tcactaatat gtctcaccat agataaatca tgagctataa ataaacaagt taaattcttt
     9001 tcttgttgca actttttaaa taaatttact atttgtgctt gtatagacac atccaaagaa
     9061 gctataggct catcagctat tataaattct gggtctactg aaagagctcg tgctatgcca
     9121 atacgctgtc tttgtccacc tgaaaaatca gatggatact tatttgcata acttctatca
     9181 agtcctacta gctttagcat ctcatatact ttattcattc tatctgtttt atttttacag
     9241 ataccttgaa tttttagtgg ctctgaaatt atatctgcaa ttgtcatacg tggatttaat
     9301 gatgaagtag aatcttgaaa aataatctgc atacttttgt ttacatcttt ttttataaat
     9361 ttgtacgttt ttttatctga tatgcaattt cctttgtaaa ttacttctcc accagtagat
     9421 tcatgtagtc ttattatagt tttaccagtt gtagattttc ctgaacccga ctctcctacc
     9481 aatccaaata tctcaccctt atatatatca aaacttatat catcaactgc ttttacagta
     9541 gtacttttgt ccaaatgaaa atattgcttt aagtttttta tttccagtat tttttctcta
     9601 ttcattagaa atcacccttc cacaatttac tctaactggt acatctactt caggtgcatc
     9661 tgggtgcaat aaccaagttg ctgcactatg tgtatcatta attttaaaca ttggtggctc
     9721 tttttcataa tctatcttaa gtgcattttt attccttatt gcaaaagcat ctccttttgg
     9781 tgggttcaat aaatttggag gcattccagg tatattatat aaatagtcat cctgcgaatc
     9841 taaagtaggt aatgaaccca acaaccccca agtatatgga tgtctagggt cataaaatat
     9901 atcctctaca gttcctattt ctacaatctt tcctgcatac ataactgcta ttctatcagc
     9961 tatagttgca actactccta aatcatgagt tataaatatt attgacaaac ctattttatg
    10021 ttgtaaatcc tttattaaat caattatttg agcttgtata gttacatcta aagcagttgt
    10081 aggttcatca gcaatcaata catctggatt acaggcaagt gctatagcta ttactatacg
    10141 ttgtctcatt ccacctgaaa aatgatgtgg aaattgttta aatcttttct caggattatc
    10201 aattccaacc aattcaataa gctctatggc tctctttttt gcttcacttt tacttatacc
    10261 ttgatgaatg attacagctt ctgcaatttg ctttcctatt gatatagttg gattgagaga
    10321 tgtcataggg tcttgaaata tcattgatat attctttcct cttatatctt ccatctcttt
    10381 ttcagacttc ttaaccaaat cacttgattt aagtaaaacc tctccatttt taatatatcc
    10441 attgtatggg agtatcctca tcaaactctt acacagtaca gatttcccac aaccagattc
    10501 tccaactata gccaatgtct cacctttttt tagattaaaa gatacatttc ttacagcctg
    10561 aacttctcct tcttctactt taaaacttac agatagatta ttaacctcca ataaatgttc
    10621 caaaaaccat caacccctat gctattcaaa ccttagggat atatttttat ccctaaggtt
    10681 tctaattaat ttcagtatta ttcaattgtc cagtcagcta tgttccagaa tatacctaca
    10741 ccatgatgac ctaaaactgt atcaggtgtt aatcctttta tgtttggttt tccaacatat
    10801 attgcatcta tataagcaat aaatgtataa ggcatatcct tagtcatttc tacttggaat
    10861 tgtttatata actttaattt ttcatcttta tcttctgttt ctcttgcttt ttgaagtatt
    10921 ttatctatag taggattaga gtaagcactg taatttgcac ctttatctgt tccaaatact
    10981 ttatatgtat ggtcatctgg gtcaaatgga cttccccaac ctattaaatg agcatcttga
    11041 ttagcccaat ctgtttcagt tacaacaaca gctttagcat caacaccaat ttcttttaac
    11101 tgttgtgcac atattttagc catatcaact ctaacttggt cactctcacc tgctgttatc
    11161 tcgaaagcta acttagtacc ttctttttca tagattccat cagaacctaa tttccaacct
    11221 agcttttcaa tttcttgttt agctttttct ggattatatt caaacttttc tatatcagga
    11281 ttattatatg gtcccatttg taaaggtgaa tatgctggta caccatgtcc taataataca
    11341 ctgtcaacta ttgcttttct atctattgca tagcttaaag catttggtaa tcctttagct
    11401 tttttatctt taaagaattt agaattaaaa ttgtataata tacctctata gtcggctgtt
    11461 ttcattatat ttactttaaa attcttttcg tctttttcaa agttagacat atctttagga
    11521 gttatttgag ctaggtctaa ttctcctgat tttaattgca ttgccttagc cttatcatct
    11581 ggaacaatct taaagactac tttatctaat cctggttctt ttacaaaata atcactattc
    11641 ttaacaagtg ttatactttg acctttgtcc catttttcaa gtttaaatgg tccagtacct
    11701 attggttttt gattaaattc atcagtagct atatccttac cttccaaagc atgttttggt
    11761 aatactccta ctgttaaata atcaagcatt gctgtatttg gagcttttaa agtaattttt
    11821 atagtattat cattaaccac atcaattttt gttatatctt cataatttga tgctatctca
    11881 gaagcattat ctggattcat tatagtttct attgtaaact taacatcatt tgctgtaaat
    11941 ttctctccat catgccactt aacatcatct cttaaattaa aagtatatgt atttgttgcc
    12001 tcatcaaatt tccagtcctt tgctagacaa ggaaccacct tattattttc atcatgagct
    12061 gttaatccat taaatattaa cgaatttatt tctccatgtt catatagtgc aggatttata
    12121 cttgtatagt cattacttcc ataaacaagt acctttccat ctttaggcgt atctgccttc
    12181 ttatctttgt ctcctccact agaacaacct gccattagac taagtatcat tactaaactt
    12241 aaaactttta acttctttaa tttcatgatg ctcccccttt tgctttttat aaaatatttt
    12301 tcaaagcttt gaccccattt atttaaatag atatttcaaa tacctcagta tttgcttatt
    12361 tagtactcaa atgctgattt ataaattact agatttttta ttattattct ttcttatata
    12421 attacctata tttgttatac acaccaatgt tgtaaccaaa aatatacccg gaatcagtat
    12481 aatccaccat ctatttgaaa gtaatgcttc ctctgacaaa gataacatac ttccccaaga
    12541 aacaatttct attggtaaac caattcctaa aaagctaagt gtcgattcag taccaatagc
    12601 tgcgcctatg tttgttacaa ccatgaacat tattgatgcc acaaaatttg gaagtaaatg
    12661 ttgctttaat acatagaaga atcctcctcc catgctctta gctgctagta tgtattcgct
    12721 atttcttatt tgtctaactt ctgttctcac tatcttagat atattcatcc aacttgttat
    12781 ccctattact atggacatag atactggatt tgaatttcca agtatagctt gtacaaatat
    12841 tataattaat attgaaggta tactaagtat gatttctgta aacctcatca ttgcatcatc
    12901 tacgagttca gatgctgaac cacttatact accataaaca attcctataa cagttgatat
    12961 tacagtggaa aatagtccta taaataaaga tatttttcct ccataccata tcatagaata
    13021 tatatctcta cccattgagt ctgttccaaa taaaaaagtt ttatttggag ctaaattaga
    13081 acttgctaaa tccatgtatg ttggttcatg tgtcattata aaagatgaga atacacttcc
    13141 aatgactatg attgataaaa taataattga tatatatggt aattctttaa tttttttaga
    13201 taaggttttc tttgtttcta tatgttcatc ttctgctact aaaatataat tttctcctac
    13261 aatttcaaaa tcactatatt gtggcttcat tagtatcacc tctatcatat ttcattctag
    13321 ggtctatttt attgtttata atttgagcta acatattccc aaatactact aaagcccctg
    13381 ttattaagca taaaactaat aacatattat aatcatgata ctttgcactt tcaaaacaaa
    13441 gtgttcctaa cccaggataa gaaaaaactt tttcaactac ataagttcct cctagtatat
    13501 gaggtatcga aatggccatt atactaatat atgatggcat tatatttctc aggcaatgct
    13561 taaaaacaat agttctatta tttaaacctt ttgctttaca taaaagcaca taatcttctc
    13621 ttatttcctc tagtaattta tttcttatca tatatgtgta ataccataga tggcttaata
    13681 taagtactgt aagaggtaag attagatgtg atatcctact taatatactg gactcttctc
    13741 ccattgaata agcaccacta cttggaagta tacttaagtt tatactaaat ataagtatga
    13801 gtactaaggc aacccaaaat gatggaatac agttggttat tgttcctaat ttgcatatga
    13861 ttctatcaat gagcttatct tcatgtaatg tacaaaatat tcctaacaat aaggctagca
    13921 caaaggtaag tatatatcct gaaccaccta atattatagt gtttatatag acatcattta
    13981 taacagaagt aacattttgt ttatatttaa atgaaattcc aaattcccct tttgatgcat
    14041 ttgaaatcca ttttatatat tgactgtata ttggttcatc caaaccaagt tttttcatag
    14101 cattttcttt ttcttgtgta ctcattcttt ctactccatc cccatagtaa gagattaatg
    14161 ggtctcctgg agcaagtctt gccatgtaaa aaacaataaa agataataaa aacattacta
    14221 aaataaactg caacaacttt ttcaatatat cctttaacaa accccaaccc ccctttgttg
    14281 caataaaaaa aaccacataa aagcaaacgt aaagtaacgt taaaaccttc gtggttccat
    14341 tatcattaaa caaaattaaa ttttctatat ttatctatga gttaatttta gcatcattta
    14401 ttaactgtgt aaacaaactt tttaaaaatt gttaaaattt catcaaatta ttttatttat
    14461 acctaaaata aaatttttat attctattct tcattatttt ctataaaatt ataaaatttt
    14521 aggttgacta tataagaaaa gtatattata ttagtttgta tgcaaaccat ttaagaggtg
    14581 attttattga aaagtaatta tgactgtttt agaatagcca tgctcttaaa agaactttat
    14641 tctaaaacta tgtacacagt agaagaaaat tttaaagaaa atggattaac acaccaacag
    14701 attatagtta taaaactgat agctcataat caagaactta caatatcaca actatgtgat
    14761 gaaatgtctt tagctaaagg tacggtttca ggcataatca gcagattaga acagattgga
    14821 tatatagaaa aattcaagaa atctaatgat aaaagaaata catacgttaa gtttacaaca
    14881 acaggatttg aatttgccac aaattttaaa attaaaatgc aagaaagttt tgatgatatt
    14941 tttaaaaatt gtgatgaaaa cgaattgagt gacttagtaa aaaatcttag aaatatattg
    15001 gcaaaagtaa aggagagata attatgaatt ataaattaat acttgcaata gtatttataa
    15061 catcagcttt aattttctac actataggtg tatttggaga aagaaaggca aaaattttaa
    15121 aaaagaaaca tgtaattata ttttggcttg gttttatatt tgatacttta ggaacattta
    15181 caatgagtaa catagccaac agccatactt ttgaagtaaa atctgcttta tcacaaaacc
    15241 ttcatagtat tacaggtctt cttgcaattg tacttatgct ttttcatgca agttgggcta
    15301 cttttgtcct atacaaagat gatgaggaaa agaaaaaatt ctttcataag tttagtatag
    15361 ttgtatggac tatatggtta gttccatatt ttataggtat gtttattggt atggctggat
    15421 agtaaaataa aaagtgtgta agaaatactg taggtaaatt tgtataagac aaatttatct
    15481 acatccttct ttcacacttt tacatattta tttataatac tccattattt ctttcatgtt
    15541 tcttaatacc tatatagaat atttaattat ttctatagta ttttattatt ttctcacact
    15601 ttttcatata tatattgttt tactattttt tcatatgtat atatgaaata ttccattatt
    15661 tctataaatg ttttattatc tttctcatac tttttcatat atatattgct tcactatttt
    15721 ttcttatttc ttcatataca tatgaaatat ttcattattt ctataatgtt ttattatctt
    15781 tctcatactt tttcatatat attgcttcac tatttttttt acttctttat taaatactta
    15841 tgtataacat acatttaata aacgctaact attaaacacc taatacttga gttactcctg
    15901 gaacttcact caccaaaaca ttttcaataa tagctttgat tgtcatagtc gctcctggac
    15961 acccactaca tgccccttgt agtctaacta aaaccacacc attttcattt acatctataa
    16021 gctctacatc tccaccatct ctttgtaata ctggttttat cttttcctca agcacttttt
    16081 caactttttc tctcatagct attcccccca gaaatatttt attaccaaaa ttataccaat
    16141 ggtttaaatt tttgtccatc taatgattta tcaaattcaa tttttgcttc atcaataatt
    16201 ttagtatcca taaatacatc ataagcacta caagccagta ctttagctga atttatcata
    16261 gcagaaaatc ctatatttga acctgctgat gaacatgact gccatgtatg acttgcaatt
    16321 ccaacaggcc aagctgccat tgcaaattga gccactggta ttacataact tacatctcca
    16381 acatcagtag aaccagctag tcctttatct ttgtcaccgt caactatttc cttgtgtata
    16441 tagctttcta caatagattt atcattttgt aatgatgttg ctacacctaa tctttcttct
    16501 ctagtaagag tatcacacaa cttttttgca aattcttctt cttcaggagt atttttaggg
    16561 caaccaacaa aaaccatatt gtcataaact aaatcagtca aacattggtt tggtatatag
    16621 tcatatattc catctgtaac cttatactcc atttcagttt ctgtcatcat tgctgctcct
    16681 ttagctactt ttattaatct atcaagaacg tgttctgcat cttttgcttt tttacctcta
    16741 ataaagtacc aactttcagc aaatccagga actacattag gtcttccacc accattagtt
    16801 ataacatagt gcattcttat agagtcaata acatgttctc tcaaataatt agctccaaca
    16861 ttcataattt caacagcatc aagtgcgctt ctaccattat gaggagcttg agctgcatgg
    16921 gctgttatcc ctttaaattt aaattttaca gataagtttg ccagacttcc acctctccaa
    16981 ggtgcattta tatcaaaagg atgccatgta aatgcacaat ctacatcatc aaaacagcca
    17041 tttatacaca taactgtctt tcctccacct tcttcttcag caggacaacc aaaatatttt
    17101 attgttccat taaatttatt ttcttctatt aattttttta tagaaacaac tgctcctaca
    17161 cctgctacac ccaacaggtt atgcccacat ccatgtccag cttttccttc taccaatggc
    17221 tttctttctg cacttacaca ttgtgatagc tctggtaaag catcatattc tccaagtatg
    17281 ccaatcacag gtttaccctc tccaaaactt gctataaatc cagtaggtaa attttctact
    17341 tcttcaattt taaatccttt tgactttagg tattcttttt gaatgctact tgcatacttt
    17401 tcttcaaagg ctaattctgg attttcccat attttcttag ataaatcaat taattcttct
    17461 cttatttctt caacattatt tatgatatac tctttcataa ttttcctcca aatatatgta
    17521 tttttgatat gctttacaga ttatattata tactaacatt ctatttattc acaatttttt
    17581 gttaattaat tttattcata tgctcttcta tggagtatat cttatcttca ctttgcctct
    17641 tcataaattt aatgtactct atcttccata aagtagtttc agttttctca tttaacataa
    17701 attctaatat attaatttct ctcattctaa tatcttcata aaatactaca ctacccaata
    17761 ttgccaattc taattcactt aatgatgcaa ataacattaa atactcttct ccaaataaac
    17821 attcagattc aagaatatcg ataaagtctt ttttagcatt gatattttca tttattattc
    17881 ttattttttc aattatagat gtactatcta atatctcatt gaatatctct ctaaacgatt
    17941 catcatccaa tttactttca tcatcaacaa tataatcttc taagtgttca tttttatttt
    18001 tactcacagt taccatactt tttaaagtgt tactttttat tgagcttagc acagattcaa
    18061 taaatatctt ttcatatttt ttaatatatc ttattacatc ttcatcttct atgcttaatc
    18121 tttttattaa tacttcaatt gcttccataa gtagtaaagt tattttttta tttattctaa
    18181 tttctctgtt aatttcttca aaatcattat aatccaaatt actatctact tcagtgttaa
    18241 aactatcatc tacttcaatc aattcaatta tattactttc ttcagagtct ataactttat
    18301 cttcagtaat attattgtac atatttttaa attgcttttc taaaaattca tactcatatt
    18361 ttgaaatttc taaattagtc acttcatttc ctaaaatagt agaaaaaata caattattta
    18421 taataagttc aaataaattt acaagtataa catcacaatc tattttataa gtcaccccat
    18481 agtcataaaa taatttttct ctatcttttt tcttaaatag gttacaaaat ctattttcta
    18541 tatctatact ctcaatataa ttcttcacat aataaatacc cttaacatcc atatcatcta
    18601 gtgccaatgg ataatctata cttactggaa cgtcctgagc ttcaaatttt acaatataac
    18661 ttttaaagaa acttgataac tccatcaagg tatcattata tgctattgaa ttagtatcca
    18721 attttctact catcattagt ttataaaaat cttttagcct aactaactct tcctctaata
    18781 tttcaatgcc tccaaggtac acttttttaa cattcttagc agtcaactcc acaactcttt
    18841 tttctatatc tccaagctta tttatatatg catcaatagc ataccatatt gaaatcaaca
    18901 gtttttcggc aacttctgtt tttactgatg tgctttgacc atttgtatat tttttaatca
    18961 aatccataag tattagagat acttcttgtt gtatattata tacttctctt tcatctatca
    19021 ctttacaatt tagacattcc ttaagtagag atatggaata ttggttcttt attaaattat
    19081 tttcgtttat attaatatta cttaaattat ttaacataca aaatctctcc tatcatatat
    19141 taaacttctc caaatttaaa tttctttgca aattcgtctg cttctcctct tacattgtcc
    19201 atattgcctg aaaatttttc ataaaattca cccagtattt ctataatctc atcatcacat
    19261 atcttgtcat cagtttcatt ttttaaataa taaaaaattt cttgcatatc attaatagcc
    19321 tctaaataat tgtccacatt tgtatattgt gatgtatata tattttctat taactgtttt
    19381 gtaactttta tatctaattc tattcttcca taccccttta aagtattatc tctcgaattt
    19441 attatatctt ttacatcaga ttcagaaaga gctagaccat aatctccaga ttctatatta
    19501 gtttcaagca gttcatttct ttctatttcc tttaaattct gatttataga actcaaaata
    19561 ataggtgata tcatttttaa attctccttt tttatttttt ccattgactt tttttgtttt
    19621 ttatgctata tttaaattag tatatattat tttaccaacc acttataata gtagtgttaa
    19681 aagtggtttt tgtcaactca aaattactat ctcaattaaa cttttcaatt ttctcaacaa
    19741 aaaaaatacc atcatttaga tatataattt tctataacaa cttttaattc agtagttatt
    19801 tactaaaatc taaacaatga tatatttatt aattttattt atcttttttt ggctctactt
    19861 ctacttttct ttacatttga gcaatttcta caagaaggtg aacagtattg agatttagaa
    19921 tttgtaggaa tataaatttt accacagtac ttgcatttat ttaaagaaga aggttcactt
    19981 gtaattgcaa aggcatagat agtctcaata gctgttttca aagaatcaaa ctcccatgca
    20041 ataattggtt tatctaattg gcttatagaa aatcctatct tttgaggatt aaaccctcca
    20101 gacataatag taacactttc agttaataga ttagatgtat ttttatatac acaaatttga
    20161 ttaaaatgct tacacatatc tttagcaaaa cttacaatcc aatctagttg ttcagtgtag
    20221 aactttgaaa atactaaatc taaaatcaat ggtctctttc caaaaaactt tggagattct
    20281 tctctctttg atacatctat accccttcta tactttttaa aatacacatc ttcatcactt
    20341 gcaaatggta taaacatgtt tacatattca tctgtactca atatattctc ttttgtaata
    20401 taattctttt caatcatcag tactctttct tctccaacta tatctctatt atatacactt
    20461 gaatatatta aacccaataa tccgtacttt tttacaaatt caagtacttc tttctttagt
    20521 ttcatttctt ttgtctcttt tgatatatct tgtaatgcct cttgtcctat gcgaattata
    20581 tctactatta tatcatctgc tacatcaaat gggttataca tggtaaacac agcatcttct
    20641 gttggaacta tatataattc atcttcttca gtaattttat attcataatc actataccta
    20701 atccaatcac ttgcaaaatt ccctattaat tctatttttt tattcataat aatcattcac
    20761 acctttcaat gtaatatata attccatatt actattacac ttttagttta ttctatattt
    20821 tctacactgt caactattct ttttatattt ttagcatatt aaaatttata atagccttta
    20881 taatggattt ctacagctaa atttcttgtt tattgatgac taattcataa ttaaatttat
    20941 aaaaactatt tacatataaa attaatctct ttataattga tatgtaatat atttttacac
    21001 ttacatatta catatcaatt ataaagagag gtatctaaaa tcatggatag aaaacgtgaa
    21061 ttaaaacaat tatacaaaga aatgaaattt gacacaggtg tattcataat aaaaaatgat
    21121 attactaaaa agatttacct tggaaaaagt aatgacataa aaagcaaatt taactctctc
    21181 aaatttcagc ttggtgctgg ctcatgtatg ataaaagaat taaataatga atggaaaaaa
    21241 tatggtgaaa aagcatttac atttgaagtt ttagaactta taaaacatga tgataacaaa
    21301 acagagaaag actatttaga agaactagat attttagaga tggtttggtt agaaaaatta
    21361 aaggaagata atacttatga aatttattcc atataaaaaa tcatataata taagaaaagg
    21421 agatagtata tcaaaataga catttagttc ataagatact tttttatata aaataaagta
    21481 tcttttcata attttaacaa tgaaaaagag gcttatctcc atttgatagc ctcttcccat
    21541 atattttata ccattataaa atgaacaatc tactaatttt caatgtatat attcatttta
    21601 ctaataacac tccattactt ctttacagca tccaaacatt gctctataat actcttatca
    21661 cttataccta ttgctgttac tttactagct ccacttaata tactcttatc acttttattt
    21721 gaaactaaaa caacaggagc attttttgca agtggtgatg caactaaagc ataaactaaa
    21781 tcgtctccac ttgttatata gaactctttt gctccattat agaacttttc aataacattt
    21841 ttattagttt gatatctatc aacaccgcca attctagttg ctctatatct gctcactatg
    21901 taatcactta atgtagactc tcctccaata gcatatactg gaacaggaga aactccaaac
    21961 catgggtcta agtctgtgtc ttcttctgct gaaggtatat cttttgtcaa tattatagga
    22021 gctccatctc tataagcaac agatgcaaca cttactgcat cagcttcatc cttaaatcca
    22081 tttactaaaa acatcttatc actgttttta attttttcta tttcgcctgc aattttataa
    22141 cttgtatcta ctctatctac accatcaatt cttataactt gcattcttct cataagtaac
    22201 attttttcta catctttact tatagaattt tctccaccta ttacatatat cttagtaact
    22261 ctattcagtc tttgaagcgt tgcttctggt atatcatcct tattagttaa taaaatagct
    22321 gcattattta ctcctgcaag accacttgca gcaatcccat ctaccattgt agaatctgca
    22381 ttgataagaa ctgctgttgt aaaatcttgc ttatctgaaa taagcccagc tgtttcatat
    22441 ttatctactc cttgaatatt ttcaatctta tctagtgcgt ttattgatgt tggaaaacta
    22501 acacttaaca gtaatgctga aatacctaac gccaatacct ttttactaat cctcataaat
    22561 tacctcccta aaacaaatat atttagtatt agtataatat aaaatcattt aaaaatagtg
    22621 taaaaatagt tacacttttt tataatttaa ataattttag actaaacaca attcatccat
    22681 taaaataact tcatttattc tatatgctaa gccatcccac gttttttttg acatatctac
    22741 agatgttttc ttatttgagt tattcaataa agattgtatc ttagaatcta ttgcattata
    22801 caaattattt tcaaaatcat ataactcttc atcttttgga ataccttctt tctccataaa
    22861 aggtagagaa acatattcta tctttccaga attattaatc tcactaccaa tccactcttt
    22921 aacaccaaga atatcagtag tgattacatc tgtgccacaa gataaagctt ctaaaactac
    22981 aactggtaat ccctcataaa atgatggtaa cacaaatatt tgtgacctgt tgaatagctc
    23041 agctaaatct ctctgttcta actttcctag gaaattcatt ttaaatggag acttacttgc
    23101 cagtttaact atctcattat atgattcgat atcgcttcca gtacctgcaa aattgacttc
    23161 tattaaatct ttactatatt ttaatttaga tatagctttt ataaatggaa tcagtccctt
    23221 tgatttacat atcttacctg caaatactat ttttatttta tcatccttta ttttataatt
    23281 tttattataa aatatatcat cattataacc actaccaatt acaactactt tactttcact
    23341 tactccaaaa gtctttataa tatcatattt ctgattttca tgaagagcaa atattaaatc
    23401 taatttcctt atatttgcta ttatgtattc tttctctaaa tcaatggtat ttaactgtct
    23461 taaacaagtt ccatgacaaa tagacataac ctttatatct tttaccattt ccctaacaaa
    23521 tgcagttaaa aggtacaaat gatgacatat tataagttct ggtttaaaat cattcatagc
    23581 cttatctata tttactttaa attgatattt caatctattt atcatgtcta tatttaaatc
    23641 tttatatctt gtactttcat atggcataga atcactcata cctacaacag gaaaatttaa
    23701 ctccccacaa ttatacttta ctggataaaa acttacttca cttggaaaac aatttacatc
    23761 atcatttaca tctatacctg caattacagc ttgcttatga ccgatttttt cgaacccttt
    23821 tatcatacca gacaaatata taccactacc tgtactattt ggtttttgtg ttattatatg
    23881 aagaattttc acttttcact tgcctccttt ctaaaattaa atagttttat acactctaat
    23941 cattatatat atctaaatat aaacaagatg aatcatcttt tactttaaat cttggcactt
    24001 taaccgcttt atagtcatta tcttcaaaat cccttacttt attatgtata tatcctatac
    24061 caaatttttc tgctatacgt atcaattctt cttcttttat tagattatat ctatcacatg
    24121 cacttgtaaa accatcactc gttagcatca attgaaaatc tttttttatt tcaatgtatc
    24181 catatataga attttccaca gcttcctcat caaaatccaa tatccaatag ccatcttttg
    24241 tatttttctt caatctatta cttactattg tatccataac actagttcta atctcatcaa
    24301 atgccatctc ttccaagtta gaaagtttac tcatttcatc aaatacagta ttatcaaatt
    24361 tacaaatact cctatctttt ataactttgg aatctccatt tttaataaac aaagcacaat
    24421 ctcctaataa aaaatactct actttatttt catgaaattt tacaacaact atagaactag
    24481 atggtaaatc taatttttca accttaaacc catttaatct tagttcatac tcttttttta
    24541 cttctattat tccagtatta ataatactct ttaatgattc ttctttattt atatttttat
    24601 ataaatacct attccaccac tgaacatacc accttgcgtc actttcagtt gacactaaat
    24661 tttttccatt aagtcctgta gcaccatcta aaacccatgc cccaaatgga tttataccga
    24721 ctacatcttc attaaattta gctgttcctt gattacataa taggctatag tttcttatat
    24781 ttaaagccat atcaacctcc aaattgaata tctaaaaata aagttctatt taaaattata
    24841 cagcaaataa aaaaatctac aacaaaactt gttgtagatt tttacttaat ctatattatc
    24901 tttatacttt cagtatttta atgatggaat aatctattct tagtaaatgc catgactatg
    24961 ttatacttat tacaagttaa tataacatta tcatcacgaa tagaaccacc tggttgtgct
    25021 ataaaactta ccccacttct ttttgcacgt tctatattat ctccaaatgg gaaaaatgca
    25081 tctgaaccaa gtgctactcc tgttaaagtt ttaagccatg ctctttttcc ttctccagtt
    25141 agaggctctg gtttttcttc aaaaaagttt tgccatatgc catcagcaag cacatccata
    25201 taatcatcag aaagatatac atcaatagtg ttatctctat ctggacgacc tatatccttt
    25261 ttaaatttta agtttaatac tttaggatgt tgacgaagat accaagtatc agctttgtta
    25321 cctgcaagtc tagtacaatg aatacgtgat tgttgacctg cacctacccc tattacttgt
    25381 ccatctttag cataacaaac agaatttgat tgtgtatatt ttaatgtaat aagagctata
    25441 attaaatcac gttttgcact atctgtaaaa attttattat cagttggaat atcttttaat
    25501 aggtcttcat ttattaatat ttcatttcta ccttgttcaa aagtaattcc ataaacatcc
    25561 tttgtttcaa taggttcagg agtatagttt tcatctattt ttacaatatt atagttgcct
    25621 ttacgctttc cttttaatat ttcaagggct tcatctgtat atcctggagc tattatacca
    25681 tcagaaacct ctcttgctat aatagttgca gtctccttat cacatacatc tgaaagtgct
    25741 ataaaatcgc cataagaaga cattctatct gctcctcttg ccattgcata agcacaagct
    25801 aatggagaaa gctctaaatc atcaacaaaa tatatttgct ttaaagtatc tgataaaggt
    25861 acccctactg ctgctcctgc tggacttaca tgtttaaatg aagttgctgc tggaagacct
    25921 gtagcttctt ttaattcctt cacaagttgc caactattaa atgcatctaa aaaatttata
    25981 tatccaggtt ttccatttaa tacttctata ggaagttctc cattcttcat ataaatcttt
    26041 gatggttttt gatttggatt acagccatac tttaattcta gttctcttgc catatactca
    26101 tccctctcta taataaatta atcataagtt tgattaatcc tatattaatt aaattattcg
    26161 tactaataaa attatatggc atatttatcc attaatcaac tatatttttt aaaactccta
    26221 ttccttcgac tttacattca actatatcgc catgctttaa atatacttga ggattcatcc
    26281 ccatagcaac ccctgctgga gttccagttg aaattatatc tcctggcttt agagtcattc
    26341 ctcttgataa atcatttatt atagtaggta tatcaaatat aaagtactct gtattagaat
    26401 tttgtcttac ttctccattt actacactac ttatatctaa ttttaaagga tgttcaaatt
    26461 cttctttatg tactatacaa ggtcccatag atgtatgtgt gtctaagctt tttcctctaa
    26521 accattgaac atgtttatct tgaagtgctc tttcacttat atcatttact attgtatatc
    26581 caaatatata ttcataagca tcttcttttg atatatttat accttcttta cctatgatta
    26641 ctgcaagttc tgattcatag tccaacttct ctaccaaatc tccatgtaag cttatcttat
    26701 catccactcc aattattttg tctgctctct tagaaaaata aattggataa tctggcataa
    26761 ctacgttttt tataactcca tctgggattt cattgacatg gtctttatag tttagaccta
    26821 aacatataat atctttttta ggtcttacaa ttggggaaca taatttaact tcttctaact
    26881 tataacactt tgcgttttca aatgaattgt tttttatttt ttctaaatct cctgtagata
    26941 cgttttcaat aaaatcattc atatcagtat ataatttact taatcctaaa ctattaattt
    27001 catatatact atttgtttct gaattaaata cacctatttt ttcttcattg ctactacaaa
    27061 aagtcacaaa tttcattttt attcctccat tcaataagtt gattaatatt atttaatttt
    27121 tattagttta tataaattgt aattc
//
LOCUS       DBGJFF010000049        26810 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.049, whole genome shotgun sequence.
ACCESSION   DBGJFF010000049 DBGJFF010000000
VERSION     DBGJFF010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26810
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.049"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            131..1324
                     /locus_tag="ACOIAF_002007"
     CDS             131..1324
                     /locus_tag="ACOIAF_002007"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HGZ2669017.1"
                     /translation="MDTLKELFKIGSGPSSSHTMGPQRAAERFKNENPDAESFRAILY
                     GSLAATGKGHLTDYIIEKTIAPKKVEIVWEEDIIKDFHPNGMKFEALDKDKNVTAEWT
                     VYSVGGGTIAEEGQRNSKSNSIYHLDTMDEIVKWCKENNKTLVDFVLECEPKDIKDYI
                     KTIKDAMRKSIDDGLSTDEIIPGKLLLKRRASTFYNAYKKDKSFSTLVYAYALAASEQ
                     NASGNIIVTAPTCGSAGVIPGIFFAMQDFYNYDDEKIIEALLVAGIIGNIIKTNASIS
                     GAEVGCQGEVGAACSMAAAAVAYLKGGTIDHIEYAAEIALEHHLGMTCDPVYGYVQIP
                     CIERNAMAAQRAYDAANYALLTDGSHSVSLDQVVETMKETGIDMMDKYKETAKGGLAK
                     HFFSC"
     gene            1530..2648
                     /locus_tag="ACOIAF_002008"
     CDS             1530..2648
                     /locus_tag="ACOIAF_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HGZ2669018.1"
                     /translation="MREASYLQEQLYKHRKSLNPIAEIGRKEYKTSKYIRDYLDKLGI
                     EYEVYLETGIVGVIEGKRPKKDIAFRADIDGLHSEEGVKHLCGHDGHATILLGLIEYL
                     NDNKEDLNDNIVFIFQPAEEGPGGAKALIDEGVLRKYNIDEIYGLHIYPELPEGYVGT
                     REGYFMAQIGDIDIEIVSKSGHGAMPQNGIDGIVIASNFVSSLQTIVSRNISPIDNAV
                     LTIGRIEGGARRNIIAENIKMEGTMRCFSPEVYEKMKLRVRELARGFELAYNCKVNIN
                     IIDDYIAVKNDKDLYQEFVKAIGSDTVVELEPLMISEDFSYYQKEVPGLFFMLGSRNE
                     EKGFVNGLHNINFNFDEKICVNALNVYSKLLKYKEAID"
     gene            2779..3024
                     /locus_tag="ACOIAF_002009"
     CDS             2779..3024
                     /locus_tag="ACOIAF_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HGZ2669019.1"
                     /translation="MKEKVQREKKITNGKNTKILTNDDIMKYEIASELGLMDKVGERG
                     WAGLTAKEAGRIGGMLTSRKKRLKKIVEEQNKKDESI"
     gene            3011..3757
                     /locus_tag="ACOIAF_002010"
     CDS             3011..3757
                     /locus_tag="ACOIAF_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGZ2669020.1"
                     /translation="MSQYSDFAFVYDELMNEVDYNGWVKYIEDIIDSEGVKVKNILEL
                     ACGTGNLTIPLTKKNYDIAGIDISDEMLSVAREKAEKEGVELVLLQQDISELDFEISD
                     LDCVLCACDGFNYIIYDDDLENIFSKTYELLKKDGIFIFDISSFYKLANILGNNMYGE
                     NREDIAYMWQNYFDEEENLIEMELTFFIKDECGKFERFEEIHQQRAYTEDEIVDMLEK
                     SGFKDIKYYGDFTFDKPSEESQRIFFVCKK"
     gene            4042..4917
                     /gene="hslO"
                     /locus_tag="ACOIAF_002011"
     CDS             4042..4917
                     /gene="hslO"
                     /locus_tag="ACOIAF_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HGZ2669021.1"
                     /translation="MRDYVLRATSGNGQVRAFVATTRNTVEEARRLHETTKVATAALG
                     RTLTATSIMGLMMKNDSDKLTVIIKGGGPIGTIIATSDSKGMVKGYVGNPQVEVEDYP
                     NGKLNVAAAVGTEGVVKVIKDLGLREPYNGTYPLVSGEIAEDFTYYFAVSEQTPSVVA
                     LGVLTKEDEVEFAGGFIVQLMPDAEEETIAKLEENVAKLPSITNMLKEGKSPEDILNI
                     VLDGLEPKILDTCEVGFMCECSKERVKTALVAIGKKSLAQIIEEDKKAEVGCQFCNKK
                     YMYSEEELLEILKEM"
     gene            5308..6108
                     /locus_tag="ACOIAF_002012"
     CDS             5308..6108
                     /locus_tag="ACOIAF_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HGZ2669022.1"
                     /translation="MESKTKYIGISILILLMIFLIAKNFNGKLEIPIPESNSRKFKSA
                     AVFYPQHQDDEVLWGGSAIVDAIKQCGVDNVYVVLVSDGSGVNVFKANTKFRNLTRKQ
                     KEELRNNEFKSALRELGVKPQNVIILADIDKKEGTHYELMEKTILDFEHKFKGNVTHI
                     AHHYKYDDHIMHRKNGEVLKKLKREGKIKDDLYFVKPKYVKYIPRNERAIYKAMDFDD
                     YNKVKSACYEYKVVNSKRKMYGIGYISAHSYFEHLLNDPELTSVLSED"
     gene            6636..8876
                     /locus_tag="ACOIAF_002013"
     CDS             6636..8876
                     /locus_tag="ACOIAF_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGZ2669023.1"
                     /translation="MNNEFIILKGCKENNLNNISLKIPKRKITIFTGVSGSGKSSIVF
                     ETIAKESQRQLNERFSTFVRSFLPKYGEVKADCIENLSTPIIIDQSRLGGNSRSTLGT
                     ITDVNSFLRALYSRFGSEYIGNANMFSFNNIDGMCPHCHGLGKKLVPNMNQILDMNKS
                     LNEGAILLSGFGVGSWHWNIFAESGFFDINKRICDYSEVELEKFLHGKAEKIKIENAG
                     QTNMTYEGLMVKFNRLYLSREGEISEATKKKLSKLLIEDRCPICDGRRLNERVYKSLI
                     NGYNIADLTSMQIDELAEVIKSIDEPEAQPLIKGIIEKLNSIIDIGLGYLTLDRETSS
                     LSGGESQRIKMVKYLNSNLVDLMYIFDEPSVGLHPKDVYKLNNLLKKLRDKGNTIIVV
                     EHDPDVIKIADHIVDVGPKAGKYGGEVVYEGSYENLLTSGTLTGNALSKFLPIKESVR
                     EHSGYLEVKNCNKNNLKNVSIKIPKGVLTLVTGVAGAGKSTLIKDEFLKQNPSAVLID
                     QSPVSANSRSSLATYSGIMNNIRSIFSKTNGVNASLFSSNSEGACDNCKGSGIVEMNL
                     AFMESIKSTCNICEGKKFKKEVLEYRFQGKNIIEVLEMSVLEAIEFFNLKQIKTKLQC
                     IEKMGIGYLTLGQTLDTLSGGECQRLKLASELNKESSVYILDEPTTGLHMADIENFIS
                     IIEEIVDNGNTVIIIEHNIDVIKRADWIIELGPEGGTKGGRVIFEGIPKQLCNNKISL
                     TAKYIV"
     gene            9291..10826
                     /locus_tag="ACOIAF_002014"
     CDS             9291..10826
                     /locus_tag="ACOIAF_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HGZ2669024.1"
                     /translation="MINDILTLLSERSDFFIKLSIEHLMISIISIIIAIVLGGIIGII
                     VSEFHNSSKPILGTINFLYTIPSISMLGFLIPFSGIGNTTAVIALTIYALLPMVRNTY
                     TGITNVDKNILEAARGMGSTDFQILYKIKLPLALPVIMSGIRNMVIMTIALAGIASFI
                     GAGGLGVAIYRGITTNNTIMTITGSILIALLALIFDFILGLVEKFFQRKRKKKNRKKV
                     FAILGSLILIATIFTSYLFNPSKTQTIHIATKPMTEQYILGEMLDILIEKNTGLNVEI
                     TQGVGGGTSNIQPAMENGEFDIYPEYTGTGWNMVLKKNELYTESMFSKLQSEYKTKLK
                     MQWTGMYGFSNSYVLAVRKEVADKYNLKNITDLKDVSSKLTFGAEYDFYEREDGYTPL
                     CNTYGLNFKDTVDLDIGLKYQAINEGKIDVMPIFTTDGQYSVSDVVVLKDDKNFYPSY
                     QCGNVVRLEVLEENPELSKVFKMVGDTLTDKEMAKMNYDVENNNLEPREVAREFLNKK
                     GLL"
     gene            10842..11603
                     /locus_tag="ACOIAF_002015"
     CDS             10842..11603
                     /locus_tag="ACOIAF_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGZ2669025.1"
                     /translation="MTPIIQFKNIKKQYNDKTIIDNLNLDIEKGEFLTVIGSSGSGKT
                     TLLKMINGLILPDGGNILINQTDIKNEDLIKLRRRIGYCVQGSVLFPHMTVEENISYV
                     PNLLSKKNKLEVKSAVNKWMEIVGLPNDMKVRYPSELSGGQQQRVGIARALASSPEIL
                     LMDEPFGAVDEITRKQLQKEIKEIHKKTGVTIIFITHDIYEALILGTKTLVLNHGVVQ
                     QYDTPENILNTPANQFVDQLLNIRKSIIDDDNLKE"
     gene            11941..12810
                     /locus_tag="ACOIAF_002016"
     CDS             11941..12810
                     /locus_tag="ACOIAF_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGZ2669026.1"
                     /translation="MDNNLITIEKTLDYIEKHLLDEMLDLNVISKEIGYSKYHLHRMF
                     TSVVGFSVHNYIQRRRLTEAARMLIFTEKPIIEIALISGYDTQRSFSRGFKKMFKRSP
                     NFFRRHQDFLPLQLKFDIHNRKKLRGDMVLDVKKVNCADINIVGYSGKVRKGFHIIGK
                     CWRDLHKNKEMIENKVDLGFLIGVSDYSNFKEIENTPTFTYIAGAQVATFGKLPNGME
                     KFVLPKSNYIVFTFIGKNEDSMQNVVEYIYQEWFPNSSCQFNENNLYDFVKYGEKTDE
                     NGLSEIQFWVPII"
     gene            13139..13390
                     /locus_tag="ACOIAF_002017"
     CDS             13139..13390
                     /locus_tag="ACOIAF_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HGZ2669027.1"
                     /translation="MNVENELLKNLDRLRTTELGVVRIKKNLSLETNDVVNWCKTKIE
                     SPNAIINRKGKNWYISVYDCIITVNAHSYTIITAHKEKK"
     gene            13502..14281
                     /locus_tag="ACOIAF_002018"
     CDS             13502..14281
                     /locus_tag="ACOIAF_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGZ2669028.1"
                     /translation="MNDTVKEKLAESLTSDTTDILPYLPYLLQDFWELGSSPEQMLQL
                     FRKHIPFNEDTKVLDLACGKGAVSVKLAKELNIHIKGIDLIPEFIDYARRKAIEYKVD
                     ALCDFEMNDANSSVKIEHDYDCVIFGAVGDILGNYKETISKLLPTIKSKGFILIDDAY
                     VVDKSTNEQLNFERVYPTYNEWCILFKELGLKLIECIPCLELENDVEEEMNWIITRAN
                     ELIKKYPSQKDMFEQYVISQKNEYTDLQQDLVGATWILQKE"
     gene            complement(14416..15066)
                     /locus_tag="ACOIAF_002019"
     CDS             complement(14416..15066)
                     /locus_tag="ACOIAF_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669029.1"
                     /translation="MNIVMINGSPKVNKNNSQYFLSELKQLIQNDNKVFTFKVDSTSK
                     YPDALNAILNCDRLIIASPLYVDGIPSHLLHFLEKCEKFLKTQNKKPIPVYAIVNCGF
                     NEGEHNHLAIQMIEHWCKKINFTWVQGIGIGAGEMFGSLESVPLGKGPKKDLGIALKE
                     LSKNILSDNIHIDDSNKSTIFISPNFPRFLYKLMGNHQWNTQAKSNGLTKSDILKK"
     gene            complement(15063..15566)
                     /locus_tag="ACOIAF_002020"
     CDS             complement(15063..15566)
                     /locus_tag="ACOIAF_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HGZ2669030.1"
                     /translation="MRLLIHDLTDKELEKLLPKLDDNIKILPNTKEIHKCIGCFGCWI
                     KTPSECVVKDYYSNMGKLINESDGIIIISRCCYGGFSPFVKNVLDKSINYILPYFVIK
                     DNMMRHKSRYDKQINLKAIFYGDCITEAEKETATKLVNGNAINFNIKEHSVCFYENIQ
                     DIRGESI"
     gene            complement(15615..16130)
                     /locus_tag="ACOIAF_002021"
     CDS             complement(15615..16130)
                     /locus_tag="ACOIAF_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGZ2669031.1"
                     /translation="MLEYIILGFLMEKELSGYDLKQIMSESTSYFFDASFGSIYPALK
                     RLETKGYIHYHEVIDGSKLKKLYSITNTGKEVFLEWLKKPINFSKTKQDHLVNIFFYK
                     YLPKEIIETNLKMLITEVELLLNKLSKQKNDAEENHCVDQYTYMYATMIYGVDYYNFV
                     INWCKDLLKNL"
     gene            16473..16934
                     /locus_tag="ACOIAF_002022"
     CDS             16473..16934
                     /locus_tag="ACOIAF_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HGZ2669032.1"
                     /translation="MRELLKWVSIINRYNTNYIDRKLADIGINNSQYFYVLHICNNPG
                     ITQDTIFEKILVNPSNITRALANLEKEGFITREPGVKDKRTWHLYPTEKSLGCYDEIL
                     KITNGYVEELLSSFEKEEQELFMSMLKKVAFLAIDMNEEAKLEAKENGRKI"
     gene            16918..18321
                     /locus_tag="ACOIAF_002023"
     CDS             16918..18321
                     /locus_tag="ACOIAF_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HGZ2669033.1"
                     /translation="MEEKYKEPLEYEKVSKLLTKYAIPSIIAMLVSALYNIVDQIFIG
                     QGVGVLGNAATNVTFPLTTICTAIALLLGIGGASKCSLELGAKNSEKATKAAGNSICL
                     MAVFGISLFIVVSIFLTPLLKFFGSTSEILPYAHTYTKITSIGLPFLIMSTGMSKLIL
                     ADGRPKASMFCMLIGAVINTILNPLFIFVFNMGIAGSALATVIGQLISFGISVHYIKN
                     FQHIKLHKKSFKLDSIIYKHIFALGSSSCFNQLAMTVTQIVMNNTLAYYGARSVYGSE
                     IPLACVGIIIKVNMIFISIIIGISQGMQPIIGYNYGAEKYNRVKEAYKFAVGSATIIS
                     VIAFLCFQFFPRQITSIFGNGNETYFIFAEHYFRTFLFLTFINGVQIVSSTFFTSIGK
                     SVLGLFTSLTRQILFLIPLIVILPMFMGIDGVMFAGPIADAAAAMVCIYFAVHEMKSL
                     TIKQNNLMSNHNKIAEV"
     gene            complement(18840..19151)
                     /locus_tag="ACOIAF_002024"
     CDS             complement(18840..19151)
                     /locus_tag="ACOIAF_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669034.1"
                     /translation="MKKIIKSFTFWFFLIALFEIYMHQIGQDSKSIVLIGLNPILSII
                     SRVDSFFVFMDSGMQIPCRTITGSISIYWYIASILSFLIYGIILDLIRIVISKIGNKT
                     K"
     gene            complement(19207..19800)
                     /locus_tag="ACOIAF_002025"
     CDS             complement(19207..19800)
                     /locus_tag="ACOIAF_002025"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HGZ2669035.1"
                     /translation="MISDSISKRRSIRKYKNQSISHETIEKIIEAGINAPSSKNRQPW
                     RFVVITEKEKESMLKAMSKGIQNEINDNGLLPGSRQHIAGANYTVEIMKQAPVTIFIL
                     NILGKSPLEKLSPEERFYEMANMQSIGAAIQNMSLTAVELGLGSLWICDVYFAYRELC
                     EWLNTDSQLVAAISLGYPDEEPLRRPRLQLSDVTEWR"
     gene            20362..20592
                     /locus_tag="ACOIAF_002026"
     CDS             20362..20592
                     /locus_tag="ACOIAF_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669036.1"
                     /translation="MRLDMDSMLYVLIYMTLLVSIIGSIFYFCKDLLNHKIKYVLIRD
                     IFVNRITYSEERLPKQYIVFQKYDIWRYCSLF"
     gene            20649..21299
                     /locus_tag="ACOIAF_002027"
     CDS             20649..21299
                     /locus_tag="ACOIAF_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HGZ2669037.1"
                     /translation="MDFISSILQGLLSILFNFTGDFGISIVLITLIVKLVLMPLSLKQ
                     RFSVKKQQELAEKMEHIKEKYKNDTKELEKQIQVSSVESMKSMLGCLIVLVQMPVVYA
                     LYHVCLDMTKEFTTIIIPWIANLGTFDNLFILPCIYTLIMLASNLVNYIPYLKINYQV
                     KFNKQMAVVTTITSLILTVRTPVAIGLYFITSAIYSLIEDICFRIYFNRKNRIVTK"
     gene            21502..22173
                     /locus_tag="ACOIAF_002028"
     CDS             21502..22173
                     /locus_tag="ACOIAF_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGZ2669038.1"
                     /translation="MAKILVVEDEKRMQNIIVEYMQKGGYTCITADDGVEALTILKSN
                     NIDLMILDIMMPYLDGFSVCRVSREMTNIPIIILTAKGEEEDKLKGYEYGADDYITKP
                     FSPKVLLAKVNALLRRYTTDIPKNSLSLGKIFIMVASRQVYVEDKLIDLTYKEFELLR
                     LFMENPNQVFSREKLLNCIWGYDFEGNTRTVDTHIKTLRKKLGSEGHHIVTLIRSGYK
                     FEVKE"
     gene            22170..23300
                     /locus_tag="ACOIAF_002029"
     CDS             22170..23300
                     /locus_tag="ACOIAF_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HGZ2669039.1"
                     /translation="MNSLRKIIEKLKNVDISNWIKQNFGLYSVRKKVFLLSKLSGALI
                     ILFYLVVEEFSINSKLEFWIWFIILVIFIMSIDFLLGKFISEPITSINKSAKSMSQLD
                     FSNPCTVNTNDEFGELSRSLNTMSTNLQQALSDLESANIQLEKDVNNERMLLEQRKEL
                     VDTISHEMKTPLGIIRAYTEGLMDEVDEEKKKNYMNVIIEETDRMNNMIVSLLDLSAL
                     EAGVSKLNPERFDFIEFVETVAGRLLIDIPDANFYFTYDLPEYEVFVVADKMRMEQVV
                     ENLVINAKKHVVYNGNLDLSITCENGLLLFEIYNDGRFIEQDEISKIWSKFYRSVQSQ
                     RTGGSGLGLAIVSQILTMQGLEYGVNNRDRGVEFYFMIPIDE"
     gene            23613..23735
                     /locus_tag="ACOIAF_002030"
     CDS             23613..23735
                     /locus_tag="ACOIAF_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669040.1"
                     /translation="MKWYTWLTIILIILLSIPLKIKFIKWWDRRQKNDKGGYDR"
     gene            23732..24577
                     /locus_tag="ACOIAF_002031"
     CDS             23732..24577
                     /locus_tag="ACOIAF_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2669041.1"
                     /translation="MIKVRELKFSYGKDKQILHGLNFEVKEGEIFGFLGPNGSGKSTT
                     QKILNGVLKGYNGHVSLFGKEVKAYTESLYQKIGVLFEFPYLYTNLSAIDNLEYFSSF
                     YPKNQRRDIYELLDLLEFKKEFINKPVSSYSKGMKQRISMARALVSNPKLLFLDEPTS
                     GLDPSGAVLFRKIIEEERKKGTTIFLTTHNMLDADLMCNNVAFIADGKIMVIDKPKNL
                     KMKNSNNKVEVEFVYNGNRDIEIVDIQELESGITFKYDEILSVHSKEPTLEEVFIKYT
                     GRMLV"
     gene            24577..25605
                     /locus_tag="ACOIAF_002032"
     CDS             24577..25605
                     /locus_tag="ACOIAF_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2669042.1"
                     /translation="MYSKFFTLVKKDFWMMIYGKFFVLTLGSLILYSCYINFGYVKFM
                     DEDSYHNNVYLYNPENINMSASPLINQVSSIGELKAKLDEDTSAVCIDMSSSKPKVIL
                     HESIKGVDNYRADYALSLLSSEKDHNSKISDNNAIIQINDKDTSEIKQRKDMTCEILF
                     FEIVAIGFLGIASLLFKEKQMGVMRITGILPVKRSLFILSKIFAFLICDICFTVLLVV
                     FNIGFFEGIKILPQVLVQASILSIIMSLIGIGCSMLLRDFKQFSLIYLVVAVFITTPI
                     FLSANTSFEIDWIKYHPFYHVYSQLKNAFFMNFSKELVYFVVCSLTIIVLFLLVKYIF
                     NKELVREG"
     gene            25608..26621
                     /locus_tag="ACOIAF_002033"
     CDS             25608..26621
                     /locus_tag="ACOIAF_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2669043.1"
                     /translation="MIGLRYQLKSIKKDKMCIISFFLPVVMAILINFMGTIDISSLGE
                     IRFGIVKDTLPVQTEQWLKKYGMVSVYDTKMHLIREIKDIKTDTIGVVRDGKGIKTVL
                     YGNEINAIKNVGKTLPEMFRLKGDLKDVEVTILPKDSILKEFQNVFIAMILITAMFMG
                     CTFNAMNIISEKEDGISFINEVLPQSRSQYIIQKVFLGLLCGCLSSMITVFICLDISI
                     LDMIMMLLLVVFSAFISSLIGLFIGKLSEGLMVGIVYIKIVMILFIGTPLLIYLLGVN
                     TSGIGNLCYIMPSVATFNGIMEIINGNVIIIKDIVILAVHCVVWFMIYIILDKKRKLS
                     FSR"
BASE COUNT        10197 a         2815 c         4723 g         9075 t
ORIGIN      
        1 taccaaaatg gaaataaaaa ataaatttta tgagaattta ataactaata attaatatat
       61 ttaggttata ataaatatgt gcgtaaaatc taacaataag caataatacc gcaaatttag
      121 gaggaataat atggatactt taaaggaact ttttaaaata ggaagtggac cttcaagttc
      181 tcatactatg ggaccacaaa gagcagcaga gagatttaag aatgaaaacc cagatgcaga
      241 aagttttaga gcaatattat atggtagttt ggcagccaca gggaaaggtc atttaactga
      301 ctatataatt gaaaaaacta tagcacctaa aaaagtcgaa attgtttggg aagaagatat
      361 tataaaagat tttcacccaa atgggatgaa gtttgaagct ttagataaag ataaaaatgt
      421 aacagctgaa tggactgtat attctgtagg tggagggact atagcagaag aaggtcaaag
      481 aaacagtaaa agcaatagta tatatcactt agatactatg gatgagatag taaaatggtg
      541 caaagaaaat aataagactt tggtagattt tgtgttggaa tgtgaaccaa aagacataaa
      601 agattatata aaaactataa aagatgctat gagaaaatct attgatgatg gactaagtac
      661 agatgaaata ataccaggca agttattatt aaaaagaaga gcaagtacat tttataatgc
      721 atataaaaaa gataagagct tctcaacact tgtatatgca tatgcactag cagcttcaga
      781 gcaaaacgct tctggtaata taatagttac agcaccaact tgtggttctg ctggagtaat
      841 accaggaata ttctttgcaa tgcaggattt ctataattat gatgacgaaa aaataattga
      901 agcactactt gtagctggaa taataggtaa tataataaaa actaacgctt caatatctgg
      961 agcagaggta gggtgtcaag gtgaagttgg ggccgcttgt tctatggcag cagcagcagt
     1021 agcatactta aaaggtggaa ctatagacca tattgaatat gcggcagaaa tagcacttga
     1081 acatcattta ggaatgactt gtgaccctgt atatggatat gttcaaattc catgtataga
     1141 aagaaatgca atggcagctc aaagagctta tgatgcagct aattatgcat tattaactga
     1201 tggctcacat tctgtctcct tagaccaagt tgttgaaaca atgaaagaaa ctggtataga
     1261 tatgatggat aagtacaaag aaacagctaa gggtggactt gctaaacatt tcttctcttg
     1321 ctaaaaataa aattttccaa taataattcc tctgttttga tttatgaaac aggggaatta
     1381 ttattaatgg tgaattattt aataatgcaa aatattttta tattcaattt agctttttat
     1441 ttttaattag tataacatgc tatataatat tttaattata aagaaatata gaaatatttt
     1501 ttaataaatt agaaattgga ggagattaca tgagagaggc atcatattta caagaacaac
     1561 tttataaaca taggaagtca ttaaatccta ttgcagagat tgggagaaaa gagtataaga
     1621 cttcaaaata tataagagat tatcttgata aattaggaat tgagtatgaa gtttatttag
     1681 aaacaggtat agtaggtgta atagaaggta aaagacctaa aaaagatatt gcttttagag
     1741 ctgacataga tggtcttcat agtgaagaag gggtaaaaca tttatgtggt catgatggac
     1801 atgctactat cttacttgga ttaattgagt atttaaatga taataaagaa gacttaaatg
     1861 ataatatagt atttatattc caaccggcag aagaaggtcc tgggggagca aaagctttaa
     1921 tagatgaagg agtgcttaga aaatacaata ttgatgaaat atatgggctt catatatatc
     1981 cagagttacc agaaggttat gtaggtacaa gagagggcta ttttatggct caaataggag
     2041 atattgacat agaaatagtg tctaaaagtg gacatggggc tatgccacaa aatggtattg
     2101 atggtatagt gatagcttca aactttgtaa gctcacttca aactatagtg tctagaaata
     2161 taagcccaat agacaatgca gtgttgacta ttggcagaat tgaaggggga gctagaagaa
     2221 atataatagc tgaaaatata aagatggaag gtactatgag atgtttcagc ccagaagtgt
     2281 atgagaaaat gaagcttaga gttagagaac ttgctagagg gtttgaattg gcttataact
     2341 gtaaggttaa tataaatatt atagatgatt atattgctgt taaaaatgac aaggatttgt
     2401 atcaagagtt tgtaaaggct attggaagtg atactgttgt agagttagag ccacttatga
     2461 tatcagaaga tttttcttat tatcaaaaag aagttccagg attatttttt atgttgggtt
     2521 caagaaacga agaaaaagga tttgtaaatg gtcttcataa tataaacttt aattttgatg
     2581 aaaagatatg tgtaaatgca ttgaatgttt attcaaaatt attaaaatac aaagaagcaa
     2641 tagattagga agataagtta taaagattaa aaagcataaa tacctaaatt taaaaaaagt
     2701 gtattaataa atacccctta catatttgta taaatttatt ttaattgaca aaaatacaaa
     2761 taagaagggg tgattttttt gaaagaaaaa gtacaaagag aaaagaagat aactaatggc
     2821 aagaatacta aaatattgac aaatgatgat ataatgaaat atgagatagc ctcagaacta
     2881 ggtcttatgg ataaagttgg tgaacgtgga tgggcaggac ttacagctaa ggaagcagga
     2941 agaattggag gtatgcttac atcacgcaaa aagaggctta aaaagattgt ggaggagcag
     3001 aataaaaagg atgagtcaat atagtgattt tgcgtttgta tatgatgaac ttatgaatga
     3061 agttgattat aatggttggg ttaaatatat agaagatata atagatagtg aaggtgtaaa
     3121 agtaaaaaat atattggaat tggcatgtgg cacgggtaat cttacaattc cattgactaa
     3181 aaaaaattac gatatagctg gtatagatat atctgatgag atgcttagtg tagcaagaga
     3241 aaaggcagaa aaagaaggag tagaattagt tttacttcaa caagatatat cagagcttga
     3301 ttttgaaata tctgatttgg actgtgtact ttgtgcttgt gatggattta attatataat
     3361 ttatgatgat gatttggaaa atatattttc aaagacatat gaattattaa agaaagatgg
     3421 aatattcata tttgatataa gttcttttta taaattagcc aacatattgg gaaataacat
     3481 gtatggagaa aatagagaag atatagcgta tatgtggcaa aattactttg atgaagaaga
     3541 gaatctaatt gaaatggaac ttacattctt tattaaagat gaatgtggaa agtttgaaag
     3601 atttgaagag atacaccaac aaagagcata tactgaagat gagatagttg atatgcttga
     3661 aaaatctgga tttaaggata taaaatatta tggggatttt acatttgaca aacctagtga
     3721 agaaagtcaa agaatcttct ttgtatgtaa aaaataagat atttttgact atataagata
     3781 attattaaaa ataaccatga tataaaaata tataaaatgc agttttatag attaaaagta
     3841 aagtgatgat attttatgac ttttattttc taataattat aaagttttat agataaaaat
     3901 taaaatataa taactattgc ttagtaaaat taaaataaaa tgggttttgc ccaaggttaa
     3961 gatagataag tatttataga ttaaaattaa ggtagtaata taataaaaat aagacaatta
     4021 acgggacatg gaggaatatt aatgagagat tatgtactaa gagcgacatc aggaaatggt
     4081 caagttagag catttgtcgc aactactaga aatacagtag aagaagctag aagattacat
     4141 gaaactacaa aagtagcaac agcagcactt ggaagaacat taacagccac atctataatg
     4201 ggattaatga tgaaaaatga tagtgataaa ttgactgtaa taataaaagg tggaggtcca
     4261 attggaacta ttatcgccac atcagactct aagggaatgg taaaaggata tgtaggaaat
     4321 ccacaagtag aggttgaaga ctatccaaat ggaaagttaa atgtagcagc agcagtaggt
     4381 acagaaggtg ttgtaaaagt tataaaagat ttaggtctta gagaaccata taatgggact
     4441 tatccattgg taagtggaga aattgcagaa gactttactt attattttgc tgtatcagag
     4501 cagactccat cagtagtagc tttaggtgtt cttacaaaag aagatgaagt agagtttgct
     4561 ggaggattta tagttcaatt aatgccagat gcagaagaag agactattgc taagttagag
     4621 gaaaatgtag ctaaattacc ttctataact aacatgttaa aagaaggaaa atcaccagag
     4681 gatatattaa atattgtttt ggatggtttg gaacctaaaa tacttgatac ctgtgaagtt
     4741 gggtttatgt gtgaatgttc aaaagaaaga gtaaaaactg ctttggttgc aattggtaaa
     4801 aaatcattgg ctcaaataat agaagaagat aaaaaagcag aagttggttg ccagttctgt
     4861 aataagaagt atatgtatag tgaagaagag ttgcttgaaa ttttaaagga aatgtagact
     4921 ataaaaatag ttgtattgat ataagtaatg ttatagtgat atagtaaagt cgtattgata
     4981 tagtaattaa agtataattt attaataata gacatattta tattaaaaaa taagtttaat
     5041 aaatttttaa gcttaataaa ttttaagaat taagaagctt tctggctgta atgtttgcaa
     5101 tgttgcagtt agaaggcttt tttattttat tatagttcgt gtcaaagatt gtatgaaaca
     5161 ataaaatatt gtaaaataat ttgtatattt agacctgatt gacacaaagg tttataagat
     5221 atttgagttg attatacgaa attctatgaa gtagtaaccc tgtgacttta atcatgggag
     5281 gttcagaaga aggagaaaat tttaaatatg gaaagtaaaa cgaagtacat aggaataagt
     5341 atattaatat tattgatgat atttttaatt gcaaagaatt ttaacggaaa acttgaaata
     5401 cctattcctg agagtaatag tagaaaattt aaatcagcag cagtgtttta tcctcaacat
     5461 caagatgatg aggttttatg gggaggaagt gccattgtag atgctataaa acagtgtggt
     5521 gttgataatg tatatgtggt attagtatca gatggttctg gagttaacgt tttcaaagct
     5581 aacacaaaat ttagaaattt aactaggaaa caaaaggaag agcttagaaa taacgagttt
     5641 aaatcagctt tgagagagct tggagtgaaa cctcaaaatg tgattatact tgcagatata
     5701 gataaaaagg aaggcacaca ttatgaatta atggaaaaga ctatattgga ttttgaacat
     5761 aagtttaaag gcaatgtgac ccatatagcc catcactata agtatgatga ccatataatg
     5821 catagaaaga atggggaagt tttaaagaaa cttaaacgtg agggtaagat aaaagatgac
     5881 ttgtattttg ttaagccaaa atatgtaaag tatataccaa gaaatgaaag agctatctat
     5941 aaagcaatgg attttgatga ctacaataag gttaagagtg cttgttatga atataaggtt
     6001 gtaaattcaa agcgtaaaat gtatggtata ggatatattt cggcacatag ttattttgaa
     6061 catcttctaa atgaccctga acttacgtct gtattaagtg aagattaatt tcttataagt
     6121 agtaagttat gtttgataag ttgattggaa ttttctatag ttttgaaggt attggaaatt
     6181 tagttttatt taaagattta aagatagagg tttttagtgt ttaaaatttt aaagttaagt
     6241 tgaataattg tatagaatta tttgtgaaag aagaaacagt gtaaaacata gtatcttatt
     6301 tatggtttta aaatcatagg caagatactt tttttatgta actaatacca ttttgagatt
     6361 attaacttaa gaattttata taatttaaca ctcatatata atgatgatat ttaggtagaa
     6421 tagatgtaat tacgcttaaa taagataact gtatttttta taaaaatcca aaccctacac
     6481 tatactacca gataaaatct acatgctata atttgacctg tgtttataaa aaaaggagta
     6541 atctaataaa atagtatttc acttatgtat aactaacaac tattattacg attttctcta
     6601 aataatttaa agaaaaataa tgcaggaggt aaaatatgaa caatgaattt ataattttaa
     6661 aaggttgcaa agagaacaat ttgaacaata tttccttaaa aataccaaaa agaaaaataa
     6721 ccatattcac aggggtgtcg ggttctggaa agtcatctat tgtatttgag acaattgcaa
     6781 aagaatcaca acgccaacta aatgaaaggt ttagtacatt tgtaagaagt tttttaccta
     6841 agtatggaga ggttaaagct gattgtatag aaaatttgtc aacaccaatt atcatagacc
     6901 aaagtagact tggtggaaat tcaaggtcaa ccctaggtac tattacagat gtaaattcct
     6961 ttttaagagc tttatattca aggtttggta gtgagtatat aggtaatgcc aatatgtttt
     7021 cttttaataa tattgatggt atgtgcccac actgtcatgg gcttggaaaa aagctagttc
     7081 ctaatatgaa ccaaattctt gatatgaata aatcattaaa tgaaggtgct atattgcttt
     7141 cagggtttgg agtgggttct tggcattgga atatattcgc tgaatcagga ttttttgata
     7201 ttaataagag gatatgtgat tattcagaag tagaattgga aaagtttttg catggtaaag
     7261 cagaaaagat taagatagaa aatgctggtc aaactaacat gacatatgag ggactaatgg
     7321 ttaaatttaa tcgcctatac ttaagtagag agggagaaat atctgaagct actaagaaaa
     7381 aattgagtaa acttcttata gaagatagat gtcctatatg tgatggaaga agacttaatg
     7441 aaagagtata taagtcttta attaatggtt ataatatagc agatttaact agtatgcaaa
     7501 ttgatgaatt ggctgaagtt attaaaagta tagatgagcc agaagcacaa cctttaatta
     7561 aaggaataat agaaaagtta aacagtatca tagatatagg tcttggatat ttaacactgg
     7621 atagagagac atcttctcta tcaggagggg aatctcagcg tataaagatg gttaagtatt
     7681 taaatagtaa tcttgttgat ttaatgtata tttttgatga accaagtgta ggacttcatc
     7741 caaaagatgt gtataaatta aataatcttt taaaaaaatt gcgtgataaa gggaatacaa
     7801 ttatagttgt tgaacatgac ccagatgtta ttaagattgc agaccatatt gtagatgtag
     7861 gaccaaaggc aggtaagtat ggtggagaag ttgtgtatga aggcagttat gaaaatctac
     7921 ttacaagtgg tactttgaca gggaatgctt taagtaaatt tcttcctatt aaagaaagtg
     7981 taagagaaca tagtggatat ttagaagtta aaaattgtaa taaaaacaat ttaaaaaatg
     8041 tatcgattaa gatacctaaa ggtgtattga ctttagttac tggagtagca ggagcaggta
     8101 aaagtacgtt gattaaggat gaatttttaa agcaaaatcc tagtgctgta cttatagacc
     8161 aaagccctgt ttcagcaaat tcaaggtcga gtttggcaac atatagtgga attatgaaca
     8221 acataagaag catattttca aaaacaaatg gcgtaaatgc ttctttattc agttcaaatt
     8281 cagaaggagc ttgtgataac tgtaagggtt ctggaatagt agaaatgaat cttgctttta
     8341 tggagagtat aaagagtacc tgcaatattt gtgaaggtaa aaaatttaaa aaagaagttt
     8401 tagaatatag gtttcaaggg aagaatataa ttgaagttct tgaaatgtct gttttagagg
     8461 caattgagtt ttttaattta aaacagatta aaacgaaatt acaatgtatt gaaaaaatgg
     8521 gtattggata ccttacttta ggacaaactc ttgatacttt gtctggagga gagtgtcaaa
     8581 gattaaagct tgctagtgaa ttaaataagg aaagttcagt gtatatatta gatgaaccaa
     8641 caacaggtct tcatatggct gatattgaga attttataag tataattgaa gagattgtag
     8701 ataatggaaa tacggtcata ataattgaac acaatataga tgtaattaaa agagctgatt
     8761 ggattattga gctgggacct gaaggaggaa caaaaggcgg gagagtaata tttgaaggta
     8821 ttccaaaaca attatgtaac aataaaatat cattaacagc aaaatatata gtataataaa
     8881 taaaatataa taaaattata atgatatttt aataaatata atgggtatat aaaatgataa
     8941 ttaaacttaa cagtaataaa taatgtgaga aatgttcaaa tttaattaaa taaagtgtca
     9001 gaaacttgtt taaattttag tattttaacg gttctgacct tttttcttat ttttatagag
     9061 aatatcgagg atggagtctc taatagttac tttttttgtg tctacttgca agtttgatta
     9121 aaaaatggct atacacactt gtgacttcag tcatgggagg ttcagattat aatcttaata
     9181 tataatttgt gaaataattt gatgacgatt tgctgatgta taaacaataa agattaacta
     9241 cattaaaatt taaatcttgt taagtaaaac ttataaaagg agagagatgg atgataaatg
     9301 atattctaac cttattatct gaaagaagtg atttttttat aaagctatcc atagaacatt
     9361 tgatgatttc tattatttct ataataattg cgattgtttt gggtggtata attggtatta
     9421 ttgtaagtga atttcataat tcttctaaac ctatattggg aactatcaat tttctataca
     9481 ccattccatc tatttctatg ctaggatttc tgataccatt ttccggaatt ggaaatacta
     9541 ctgctgttat tgcacttacc atttatgcat tattaccaat ggttagaaat acatatactg
     9601 gaattactaa tgtggataaa aatattcttg aagcagctag gggaatggga agtacagatt
     9661 ttcagatatt gtataaaata aaacttccgt tagccctacc tgttattatg tctggtatta
     9721 gaaatatggt gattatgaca attgcattag ctggtatcgc ttcatttata ggtgctggag
     9781 gtctaggtgt agccatatat agaggaatca cgacaaataa cacaataatg acaattactg
     9841 gaagtatact tattgctcta ttagctttaa tatttgattt tattttaggg ctggtagaaa
     9901 aattttttca acgaaaaaga aaaaagaaaa ataggaaaaa agtttttgcc attctaggaa
     9961 gccttattct tatagcaact atctttacgt cttatttatt taatccttca aagactcaaa
    10021 ctattcatat tgctactaaa cctatgactg aacaatatat tcttggtgag atgttggata
    10081 ttttaattga aaaaaacaca ggtctaaacg tggaaattac acaaggtgta ggagggggaa
    10141 catctaatat ccagccagct atggagaatg gagaatttga catttatcca gagtatacag
    10201 gtacaggctg gaatatggta cttaagaaaa atgaattata tactgaaagt atgtttagta
    10261 aactacagtc ggaatacaaa actaaactta aaatgcaatg gactggtatg tatggttttt
    10321 ctaatagcta tgtattagca gttagaaaag aagttgcaga taaatacaat ttaaaaaata
    10381 taacggactt aaaagatgta tcttctaaat tgacatttgg agctgaatat gacttttatg
    10441 agagagagga tggatatact ccattgtgta atacatatgg actgaacttt aaagatacag
    10501 ttgatttaga tattggatta aaataccaag ctatcaatga aggtaagatt gacgtaatgc
    10561 ctatatttac aactgatggt cagtatagtg tttcagatgt tgtagttcta aaagatgata
    10621 aaaatttcta cccatcttat caatgtggaa atgttgttcg tttggaagta ttagaggaaa
    10681 atccagaact atcaaaggta tttaagatgg ttggagatac tttgactgat aaagaaatgg
    10741 ctaaaatgaa ttatgatgta gaaaacaaca atttagaacc tagagaagtt gctcgtgagt
    10801 ttctgaataa gaaaggtctt ttataaattg gaggaaatat aatgacacct attatacaat
    10861 ttaaaaatat taaaaaacaa tataatgata aaactataat agataatctt aatttagata
    10921 tagaaaaagg agagtttttg actgtaattg gttcgtctgg ttctggtaag acaacactct
    10981 taaaaatgat taatggtctg attttgccag atggaggaaa tattttgata aaccaaacag
    11041 atatcaaaaa cgaggactta attaaattaa gaagaagaat aggatattgt gtacagggaa
    11101 gtgttctttt tcctcatatg actgttgaag aaaatatttc atatgtacca aatctcctaa
    11161 gtaaaaagaa taaattagaa gtaaaaagtg ctgtaaacaa gtggatggaa atagtgggat
    11221 tgccaaatga tatgaaagtg cgttacccat cagaattgtc aggtggtcag caacaacgtg
    11281 tagggattgc aagagcttta gcttcttctc cagaaatttt attaatggat gaaccttttg
    11341 gtgcagttga tgagattaca cgaaagcaat tacaaaaaga gataaaagaa attcataaaa
    11401 aaacaggagt tacgattatc tttattacac atgatattta tgaagctttg attttgggaa
    11461 caaagacttt ggtattaaat catggagttg ttcaacagta tgatactcct gagaatattt
    11521 taaatactcc tgctaaccaa tttgtagacc agttattgaa tataagaaaa tctataatag
    11581 atgatgataa tttaaaagaa taaatttaaa tttaaaatca aattgttgtg ataaaaacta
    11641 ttgatttaga gctaacttca agttgtatac ttgaagtgga aaagttagat taaggtatag
    11701 aagggaattt agcagttggc tgtagacaag gtaactgctg aaggtgattt caataaatgg
    11761 ctggaacctg tatctgttga agattataat aaattgaaat aagaagctca atatacagag
    11821 tagataggca cttcaaatag aagtgtcttt tgtgtgctat aaaataaatc aaaatattag
    11881 caaagaatgt gaaaaacaaa atgaaatagt atgctatctt taaaatagga ggggtgctat
    11941 atggataaca atttgattac cattgaaaaa actttagatt atatagaaaa acatctttta
    12001 gatgagatgt tagatttaaa tgtaatttct aaagagatag gctattcaaa ataccattta
    12061 catagaatgt ttacatcagt tgtcggattt agtgtacata attatataca aagacgtaga
    12121 cttactgaag cagcgagaat gctaattttt acagaaaaac caattattga gattgcttta
    12181 atctcggggt atgatacaca acgctctttt tcaagagggt ttaaaaaaat gtttaaacgt
    12241 agtcctaatt tttttagaag acatcaagat tttttaccac tacaattaaa atttgatatt
    12301 cataatagaa aaaaattacg tggagatatg gttttagatg ttaaaaaagt taattgtgca
    12361 gatataaata ttgttggcta ttctggtaaa gtaagaaaag gatttcatat tattggaaaa
    12421 tgctggaggg atttgcataa aaataaagag atgatagaaa ataaggtaga tttaggtttt
    12481 ttaattggag ttagtgatta tagcaatttt aaagagattg agaatactcc tacatttaca
    12541 tacattgcag gtgctcaagt agctactttt ggcaaattac ctaatggaat ggaaaaattc
    12601 gtattgccga aaagtaatta tattgttttt acttttattg ggaaaaatga ggatagtatg
    12661 caaaatgttg tagaatatat ctatcaagaa tggttcccta attcaagttg ccagtttaat
    12721 gaaaataatt tatatgactt tgtaaaatat ggagaaaaaa ctgatgagaa tggtttgagt
    12781 gaaattcagt tttgggttcc aatcatttaa aataaggtat tatgtataaa gtagatggat
    12841 ttatataggc aaagttttac agcagtatgt atttataaat aatttatagt ttgtaggaaa
    12901 gaaataattt tactataaga ttatgataaa atacacaata ctatgacaaa atagtgttaa
    12961 ataatagatt taattttgca taggaaaagt tctataatgg tgtgtattca taaataattt
    13021 ataatctgta ggaaagaaat agttttacta taagattatg ataaaataca caatactatg
    13081 ataaaatagt actaaataat agatttaatt ttgcaaagga taaggtgttt taataaaaat
    13141 gaatgtagaa aacgaattac ttaaaaacct agatagattg cgtacaacag agttaggcgt
    13201 tgtaagaatt aagaaaaatc tttctttgga gacaaatgat gtagtgaact ggtgtaaaac
    13261 taaaatagaa tctccaaatg ccattattaa tagaaaggga aaaaactggt atataagtgt
    13321 atatgattgt attataacag taaacgctca cagttataca attattacag ctcataaaga
    13381 aaagaaatga attgcaaaat acaaaggtca aaagcatttt ccactgtttg tagtacctat
    13441 gaagaagtat ataaaataat attacttttg aataataatt taaattttgg aggttgaaaa
    13501 aatgaatgat actgtaaaag aaaaattggc agagtcacta acttctgata ctactgatat
    13561 tttaccttat ttgccttatt tattacaaga tttttgggaa ttaggaagtt caccagaaca
    13621 aatgttacaa ttatttagaa agcatatacc ctttaatgag gatacaaaag ttttggattt
    13681 agcttgtgga aaaggtgctg tatctgtgaa gcttgcaaaa gagttgaata tacatatcaa
    13741 aggtattgat ttgatacctg aatttattga ttatgcgaga agaaaggcta ttgaatataa
    13801 ggttgatgct ttgtgtgatt ttgaaatgaa tgatgctaat agttctgtaa aaattgagca
    13861 tgattatgat tgtgtgattt ttggagctgt aggggatatt ttaggtaatt ataaagaaac
    13921 tataagtaag ctattaccta caataaaaag taaggggttc attttaattg atgatgcata
    13981 tgtagtagat aaatctacaa atgaacagct taattttgag agggtgtatc ccacttacaa
    14041 tgagtggtgt atattgttca aagaattagg tctgaaacta attgaatgta taccatgttt
    14101 agaacttgaa aatgatgttg aagaggaaat gaactggatt ataactcgtg caaatgagct
    14161 aattaaaaaa tatccttctc aaaaagatat gtttgaacaa tatgttatta gccaaaaaaa
    14221 tgaatatact gatttacagc aggaccttgt tggagcaact tggattttac aaaaagaata
    14281 agataaatat tagactagat aatcaatgtt gtagtgaatt atcccatgtc tagtgaatat
    14341 aaataacata aaaaaccgtc aagatgaata gtttattcgt gcttgacggt ttttagtatt
    14401 tgttgaagta aaattttatt tctttagtat atcactttta gtcaatccat ttgatttagc
    14461 ctgagtattc cattgatgat tccccataag tttatatagg aatctaggga agttaggtga
    14521 aataaaaata gtacttttgt tggaatcatc tatatgtata ttatcactaa gtatattttt
    14581 tgaaagttct tttaatgcaa ttcctaaatc ttttttaggt cctttaccca aaggtacgga
    14641 ttctagtgaa ccaaacatct ccccagctcc aattccaata ccttgaaccc atgtaaagtt
    14701 gattttttta caccaatgct ctatcatttg aattgccaaa tgattatgtt caccttcatt
    14761 gaatccacag ttaactattg catacactgg aataggtttt ttattttgtg tttttaaaaa
    14821 tttttcacat ttctctaaaa aatgaagtaa atgagatgga ataccatcta cataaagagg
    14881 agatgcgatt attaatctat cacaattaag tatggcatta agagcgtcag gatacttaga
    14941 tgtgctatct actttaaatg taaaaacttt attatcattt tgaataagtt gttttaattc
    15001 agataaaaaa tactgtgaat tatttttatt tacttttgga ctaccattta tcattactat
    15061 attcatatgg attcacctct tatatcttgt atattttcat agaaacaaac agagtgttct
    15121 tttatattga aatttatagc atttccattt actaatttcg tagcagtttc tttttcagct
    15181 tctgtaatac aatctccata gaatatagct tttaaattta tctgtttatc atatctgctt
    15241 ttatgacgca tcatattatc ttttattaca aagtatggaa gaatataatt aatgctttta
    15301 tcaagtacgt ttttgacaaa agggctaaac cctccgtaac agcatctgct tattataata
    15361 attccatcag attcatttat aagtttaccc atgttgctgt aataatcttt tacaacacat
    15421 tcagaaggag ttttaatcca gcaaccaaaa caaccaatac atttgtggat ttcttttgtg
    15481 tttggaagta tttttatatt gtcatcaagt ttgggtaata acttttctaa ttctttgtca
    15541 gttaaatcat gtattaaaag cctcataaag tccatctcct tttattatgt atgtttactt
    15601 ctaaaaatta tatcttatag atttttcagt aaatctttac accaattaat aacaaaatta
    15661 taatagtcaa ctccataaat cattgttgca tacatatatg tatattggtc aacacaatga
    15721 ttttcttctg catcattttt ttgtttactt aatttattta gaagtaattc tacttcagtt
    15781 attaacattt ttaagtttgt ttcaattatt tctttgggta gatacttgta aaagaatata
    15841 ttaacaaggt ggtcttgctt tgttttagaa aaatttatag gtttctttaa ccattcaaga
    15901 aatacttctt taccagtatt agttatggaa tatagttttt ttaatttgct accatctata
    15961 acttcgtgat agtgtatata tccttttgtt tctaatcttt taagtgcagg atagatactt
    16021 ccaaagctgg cgtcaaaaaa gtacgaggta ctttctgaca ttatttgttt taagtcgtat
    16081 ccacttaatt ctttttccat taaaaatcct aaaataatgt attctaacat attacctcct
    16141 ggtatcaatt agatatatat cgattcgata tacttattat tacagataat aaatcttacg
    16201 tcaagatgct ttttaaaata agctaaaaag aattaggagt tattgataaa tatgcaaaaa
    16261 aattaatata aaaggtaatt aaatatcaat gaagatttaa aaatagttaa atcaaagtaa
    16321 gagtctaata tataaaattt atagtaactt tatggtttaa atataaactt gtgtgctatt
    16381 gacaaagtat aaaattagca gtagaataat tgcatgagca aatagattgt tgcgaacgca
    16441 aaaaaaggac aatagatatt aaaggagaga atatgagaga gttattaaaa tgggtttcca
    16501 ttataaatag atacaataca aattatattg acaggaaatt ggcggatatt ggaatcaata
    16561 atagccaata tttttatgta ttacacatat gtaataatcc aggtattaca caggacacta
    16621 tatttgagaa aattcttgtg aatcctagta atataacaag agctttggca aatttagaaa
    16681 aagaaggctt tataacaaga gaaccaggtg taaaggataa aagaacatgg catttatatc
    16741 ctacagaaaa atctttagga tgttatgatg aaatactaaa aataacaaat gggtatgttg
    16801 aggagttgtt gagttcattt gagaaagaag agcaagagtt gtttatgtca atgttaaaga
    16861 aagttgcatt tttggctatt gatatgaatg aagaagcaaa attggaggcg aaggaaaatg
    16921 gaagaaaaat ataaggagcc attggaatat gaaaaggttt caaagttatt aactaagtac
    16981 gcaattccta gtattatagc aatgttagta agtgctttat ataacattgt tgaccaaata
    17041 tttattgggc aaggtgttgg tgtattgggt aatgctgcta caaatgtcac ttttccacta
    17101 acaacaatat gtactgcaat tgcattattg ttaggaattg gaggagcgtc taagtgttca
    17161 ttggaattag gtgctaaaaa ttcagaaaaa gctacaaaag cagcaggaaa ttctatatgc
    17221 ttgatggctg tttttggaat aagtttattt atagtagtat caatattttt aacaccacta
    17281 ttaaaattct ttggctctac atcagaaata ctaccttatg cacacacata tacaaaaatt
    17341 acatcaattg gattaccgtt tcttataatg tccactggaa tgagtaagtt gatacttgca
    17401 gatgggagac cgaaagcttc tatgttttgt atgttaatag gagcagttat taatacaatt
    17461 ttgaatccat tattcatttt tgtttttaat atgggaattg ctgggtctgc tttagcaact
    17521 gttattgggc aacttatatc ttttggaata agtgttcatt atataaagaa ttttcaacat
    17581 attaaattac ataagaaaag ttttaaatta gatagtataa tttataaaca tatatttgcg
    17641 ctaggttcaa gctcatgttt taatcaatta gcaatgactg taacacagat agttatgaat
    17701 aatacattgg catattatgg ggctaggtca gtttatggaa gtgagattcc acttgcttgt
    17761 gtcggtatca ttattaaagt aaatatgatt tttatatcta taattattgg tatatcacaa
    17821 ggtatgcaac caattatagg gtataattat ggggctgaaa agtataatcg tgtaaaagaa
    17881 gcatataagt ttgcagtggg aagtgcaact attatatctg ttattgcatt cttatgtttc
    17941 caattcttcc caagacaaat tacgtctata tttggtaacg gaaatgagac ttattttata
    18001 tttgcagaac attattttag aactttcttg ttcttaacat ttataaatgg tgttcaaata
    18061 gtatcatcaa ctttctttac atcaattggg aaatcagtgt tgggactttt tacttctttg
    18121 actcgtcaaa ttttattttt aattccattg atagtaattt taccaatgtt tatgggaatt
    18181 gatggagtaa tgtttgcagg accaatagca gatgcagcag cggcaatggt atgtatatac
    18241 tttgcagttc atgaaatgaa atcattgact ataaagcaaa ataatttgat gagtaaccat
    18301 aataagattg cagaggtata agacaatatt taagaagtat agaaaattaa tttaaagaag
    18361 tataataaac taatttagga agtatagaaa attaatctaa agaagtataa taaactaatt
    18421 taggaagtat agaaaattaa tctaaagaag tataataaac taatttagga agtatagaaa
    18481 gctaatctaa agaagtataa taaactaatt taggaagtat agaaaactaa tctaaagaag
    18541 tgtaataaac taatttagga agtatagaaa gctaatttaa agaagtataa taaactaatt
    18601 taggaagtat agaaagctaa tctaaagaag tataataaac taatttagga agtatagaaa
    18661 attaatttaa agaaatacaa tacattaatt tttaagtaac attaaattaa tgtaagtaaa
    18721 ataatgttca tatgaagatt taaaggtgca ttttctgaag ctaagtatat gttgtagagt
    18781 gttatgtttc tctatccggt gaagtctaat agctcaagaa aatgcactat ttatgatgat
    18841 tattttgttt tatttccaat tttggaaata actattctaa ttaaatctaa aattatacca
    18901 taaataagaa aagaaagtat agatgctatg taccaataaa tcgaaattga ccctgtaatt
    18961 gttctacatg ggatttgcat acctgaatcc ataaaaacaa aaaaactatc aactctggaa
    19021 ataatagaca aaataggatt taaacctatt agaacaatac tttttgagtc ttgaccaatt
    19081 tgatgcatat aaatctcaaa taatgcaatt aaaaaaaacc aaaatgtaaa agatttaata
    19141 atttttttca tcaaaatacc tcacataata taaattctgt ttgcactatt tgcttactac
    19201 aactatttat ctccattcag tcacatcact taattgtaat ctaggtcttc ttaatggttc
    19261 ttcatcagga taacccaaag atattgctgc cactaattga ctatctgtat ttaaccattc
    19321 acataattca cgatatgcaa agtatacatc acaaatccaa agactaccta gacctaattc
    19381 cacagctgta agcgacatat tctgaattgc agctccaatt gattgcatgt ttgccatttc
    19441 atagaatcgt tcttcagggg atagcttttc taaaggtgat ttacctaata tatttaaaat
    19501 aaaaattgtt actggagctt gtttcatgat ttcaactgtg taatttgcac cagcgatatg
    19561 ttgacgactt cctggaagaa gcccattatc attaatttca ttttgaatac ctttgctcat
    19621 ggctttaagc atagattctt tttccttttc agtgatgaca acaaaacgcc atggttgcct
    19681 gtttttagat gaaggtgcat ttattccagc ttcaattatt ttttctatag tttcatgtga
    19741 aatgctttga tttttatatt ttctgatact tcgtcttttt gatatagaat cacttatcat
    19801 tttaagtaac ctccgtttat tttttgaaaa aatctattta atttttattt tctattttaa
    19861 ttattttagc attaatcaga aaaaaagtca ttgattttag tatacaatta ttaatgatta
    19921 tattaagaat tgtatcaaaa agtatgtgat tatactaagg gattatatta aacattatat
    19981 ttaaaattgt atgaaaggtt gtatttaata ttatattgag agatatatat attaagattt
    20041 atatatatta agatttatta ttatattgag ccttatatta aattaaggac tataatttga
    20101 aaataagcta taaaaactta atattgtttt gttcagagat aaaaaaaata ctttaggtga
    20161 tttttattaa aaattaagag tgataagatt atatacttta taaatgtaaa tatgacgaat
    20221 aaaaatgata aatataataa tatatatatt tacattagga gttttctata ctataattat
    20281 ttaaaactaa atataaatgt tggaattatt taggacttga aattgtctaa aaataaatat
    20341 aagtgatgaa gaggtgatgt gatgagatta gatatggata gtatgttata tgtactaatt
    20401 tatatgacat tattggtatc tatcattgga agtatatttt acttttgtaa agacctctta
    20461 aatcataaaa ttaagtatgt tttaataaga gacatatttg taaatagaat tacatattct
    20521 gaagaacggt tgcctaaaca gtatatagtt ttccagaaat atgatatttg gaggtattgt
    20581 agtttgtttt aatttgaaat gaatctatta taaattctaa agtttaaaaa taaaaaggag
    20641 agaataaaat ggatttcatt tcaagtatat tgcaaggatt attaagtata ttatttaatt
    20701 ttacaggtga ttttggaatt tctattgtac ttataacttt aatagttaaa ttagtactta
    20761 tgccactatc attaaaacaa agattttctg taaaaaaaca gcaagaatta gctgaaaaaa
    20821 tggaacatat aaaagaaaag tataagaatg atactaaaga attggaaaag caaatacaag
    20881 taagttctgt agaaagtatg aagagtatgc tgggatgtct tatagttttg gttcaaatgc
    20941 cagtagtata tgctttatat catgtatgtt tagatatgac aaaggaattt acaacaatta
    21001 taattccatg gattgctaat ttaggtacat ttgataattt gtttatatta ccttgtatat
    21061 atacattaat tatgttagct tcaaatttag tgaattatat accatattta aaaataaatt
    21121 atcaagtaaa gtttaacaaa caaatggctg tggtgactac cataacaagt cttatactaa
    21181 ctgttagaac tccagtagcc ataggtctgt actttataac aagtgcaatt tattcattaa
    21241 ttgaagatat ttgttttaga atatatttta atcgaaaaaa tagaatagtg acaaaataat
    21301 ataattatta ttagaaatat tttagcctgc taacatagaa aaagttagta ggctattttt
    21361 atctatgata tcaattccat taagaatagt ctaaaaaata ctatcaaaat gctataaaaa
    21421 atactataaa aaaatagttg aaaaataaat aaattaatat aaactaaaat aaagcctaca
    21481 aaaaagaggt gataaataga aatggctaaa atactagttg tagaagatga aaaaagaatg
    21541 caaaatatta ttgtagaata tatgcaaaag ggaggatata cttgtataac tgctgatgat
    21601 ggagtcgagg cacttacaat attaaagagc aacaatatag atttaatgat attggatatt
    21661 atgatgccgt atttagatgg attttcagtg tgtagagttt cacgtgaaat gactaatata
    21721 cctattatta tattgacagc taaaggtgaa gaggaggata aattaaaggg ttatgaatat
    21781 ggcgcagatg attatataac aaaaccattt agcccaaaag tacttttagc aaaagttaat
    21841 gccttgttac gtagatacac tactgatatt cccaaaaatt cattgagttt aggaaagatt
    21901 tttattatgg tggcatcaag gcaagtgtat gtagaagata aattgattga tttaacatat
    21961 aaagaatttg aattattgcg tttatttatg gaaaacccta atcaagtttt ttccagagaa
    22021 aaacttttaa attgtatatg ggggtatgat tttgaaggaa acacacgaac tgtagataca
    22081 catataaaaa ccttacgcaa aaagcttggt agtgaaggac accatatagt tacattaatt
    22141 cgttcaggat ataagtttga ggttaaggaa tgaatagttt aagaaagatt atagaaaaac
    22201 tgaaaaatgt agatatttca aattggatta aacaaaattt tggactatat tcagttagaa
    22261 aaaaagtgtt tttactttct aaattatcag gagcgttaat tatattattt tatctggttg
    22321 ttgaagaatt ttcgataaac tctaaattag aattttggat ttggtttata atactggtta
    22381 tatttattat gagtattgat ttcttattgg gaaaatttat ttcagaacca atcacatcta
    22441 taaataaatc tgcaaaaagt atgtctcaac ttgatttttc taatccatgt actgtaaata
    22501 ctaatgatga atttggagaa ctttctagga gcctgaatac aatgtctaca aatctacaac
    22561 aggcattatc agacctagaa agtgcaaata ttcaacttga aaaggatgta aataatgaaa
    22621 gaatgctatt ggaacaaaga aaggagttgg tagatacaat ttcacatgaa atgaagactc
    22681 cacttggcat tatacgtgcg tatacagagg ggcttatgga tgaagttgat gaagaaaaaa
    22741 agaaaaatta tatgaatgtt ataatagaag aaactgatag aatgaacaat atgattgtat
    22801 cattattaga cttatctgca ttggaagcag gagtttctaa acttaaccca gagcgttttg
    22861 attttataga gtttgttgaa actgtggcag gtcgtctttt aattgatata ccagatgcca
    22921 atttttattt tacatatgat ttgccagagt atgaagtttt tgtagtggca gataaaatga
    22981 gaatggaaca agtagtagag aatctagtta ttaatgcaaa gaagcatgtt gtatataatg
    23041 gtaatttaga tttatcgatt acatgtgaaa atggcttgct tttatttgaa atttataatg
    23101 atggtaggtt tatagagcaa gatgagattt caaagatttg gtctaagttt tatcgtagtg
    23161 ttcaatctca gcgtacagga ggttcaggtc tagggcttgc aattgtttca caaatactta
    23221 caatgcaagg gttagaatat ggagtgaata atcgtgatag aggagtagag ttttatttta
    23281 tgattccaat tgatgaataa tttagtatta atttataaat tataagtatc tgatgtaaaa
    23341 tatttttatt tgtatatgta agcatttaaa gagaataaaa taaaaaaact taatgaaaaa
    23401 agcttaatga aaaagtctaa taaaaagctt aatgaaaagc ttaataaaaa gtcttgtttt
    23461 cctgatgaaa acaagacttt ttattaagct ttttattatt tcacttcgat ttcacaagag
    23521 agacactctg atttcaccaa atagaaataa aataggttaa ataaattaaa aaatagaagt
    23581 gtaattaaat aaggttacaa gggggagtac ttatgaaatg gtatacatgg ttgactatta
    23641 tattgataat attgctttct attccgctta aaattaaatt tataaaatgg tgggatagac
    23701 gacaaaaaaa tgataaagga ggttatgata gatgataaaa gtaagagagt taaaattttc
    23761 ttatggaaaa gataaacaga tattgcatgg acttaatttt gaagtaaaag aaggagaaat
    23821 ctttggattc cttggaccta atggttcagg aaaatccaca acacagaaaa tcttaaatgg
    23881 tgttttaaaa ggttacaatg gtcacgtatc cctttttggt aaggaagtta aagcatacac
    23941 agaatcttta tatcaaaaga ttggtgtttt atttgaattt ccttatttat ataccaattt
    24001 aagtgcaatt gataatttag aatatttttc gtctttttat cctaaaaatc aaaggagaga
    24061 catatatgaa ctcttagatt tgttagagtt taagaaagag tttataaata agcctgtatc
    24121 atcctactca aaaggtatga agcaacgtat tagcatggca agagcattgg taagtaatcc
    24181 aaagttgtta tttttagatg agcctactag tggacttgac ccatctggag ctgtactatt
    24241 tcgtaagatt attgaagaag aacgaaaaaa aggaacaact atctttttga ctactcataa
    24301 tatgctagat gcagatttaa tgtgtaacaa tgtggctttt attgcagatg gaaagattat
    24361 ggtaatagat aaacccaaaa atttaaaaat gaaaaatagc aataataaag ttgaagtgga
    24421 atttgtatat aatggaaatc gagatattga aattgtagat atacaagagt tagaatctgg
    24481 aataactttt aaatatgatg aaatcttaag tgtacattca aaagaaccta cactagaaga
    24541 agtttttatt aaatacacag gaaggatgtt agtgtaatgt attctaaatt ttttacctta
    24601 gtaaaaaaag atttttggat gatgatttat ggtaaatttt ttgtgctgac acttggctct
    24661 ctcatacttt attcgtgtta tattaatttt ggttatgtaa agtttatgga tgaagatagt
    24721 tatcacaata atgtatatct atataatcca gagaatataa acatgagtgc atctccatta
    24781 attaatcaag tttcttctat aggtgaatta aaagcaaaac ttgatgaaga tacaagtgca
    24841 gtttgtattg atatgagttc cagtaagcct aaagtaattt tacatgaaag tattaaagga
    24901 gttgacaatt atagagctga ctatgcattg tcattattat catcagaaaa agaccataat
    24961 agcaaaatat cagacaacaa tgcaataatt caaattaatg ataaggatac atctgagata
    25021 aaacaaagaa aagacatgac ttgtgagatt ttattctttg aaattgtagc tattggattt
    25081 ttagggattg cctctttatt atttaaagaa aagcagatgg gagtaatgcg tattactggt
    25141 attttacctg taaaaagaag tctttttata ttatcaaaga tatttgcttt tttgatatgt
    25201 gacatatgtt ttacagttct tttagtagta tttaatattg gattttttga aggaataaaa
    25261 atacttccac aagtattagt tcaagctagt attttatcaa ttattatgtc tttgattgga
    25321 ataggatgct caatgttatt gagagatttt aagcaatttt ctcttatata tcttgtggtt
    25381 gctgtattca taactacacc tatatttcta tctgcaaata catcttttga aatagactgg
    25441 attaaatatc atcccttcta tcatgtttat agtcagctta aaaatgcgtt tttcatgaat
    25501 ttttcaaaag aattagttta ttttgtagtg tgttctttga caataatagt tctattttta
    25561 cttgtaaaat atatttttaa taaagagttg gttagggagg gataatcttg ataggattac
    25621 gttaccagtt aaagagtata aaaaaagata aaatgtgtat aatttccttc tttttaccag
    25681 ttgttatggc gattttaata aattttatgg gaacaattga tatatcttca ttaggagaga
    25741 tacgttttgg tatagtgaag gatacacttc ctgtacaaac agagcagtgg ttgaagaagt
    25801 atggtatggt aagtgtatat gacacaaaaa tgcatttaat acgtgaaatt aaggatatta
    25861 aaacggacac aattggagtt gttagagatg gtaaagggat aaaaactgtg ctttatggaa
    25921 atgagattaa tgctataaaa aatgttggaa agacattgcc agagatgttt agactaaagg
    25981 gtgatttaaa agatgttgag gttacaatat tgcctaaaga tagtatttta aaagagtttc
    26041 aaaatgtttt tattgccatg attttaatta ctgctatgtt tatgggatgc acctttaatg
    26101 caatgaatat tatatctgaa aaagaagatg gaatatcatt tataaatgaa gttttgccac
    26161 agagtagaag tcagtatatt atacaaaaag tatttttggg tcttttatgt gggtgtttat
    26221 cttcaatgat aactgtattt atatgtttgg atatttcaat attagatatg ataatgatgt
    26281 tgttactcgt tgtattttca gcatttattt catcacttat aggtttattt attggaaagt
    26341 tgtcagaagg tcttatggta ggaattgttt atattaaaat agtaatgatt ttatttatag
    26401 gtactccttt gttaatatat cttctagggg ttaataccag cggtataggg aatttatgct
    26461 atataatgcc atcagttgct acatttaatg gtattatgga gattattaat ggtaatgtca
    26521 ttattataaa agatattgta attttggctg tacattgtgt agtatggttt atgatatata
    26581 ttattttaga taaaaaaaga aagttatctt ttagtagata actcagtata attataattg
    26641 ggacaattta ataggtgatt taatatactt tgtatagtgt aatttatgta gtgcaatttg
    26701 tatactttaa tttatgtagt gtaatagtta gttttatttt ataattattc aatataattt
    26761 agtaagaagt tcagtacaat tagagctact tagtggaaaa cttaataggt
//
LOCUS       DBGJFF010000050        26772 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.050, whole genome shotgun sequence.
ACCESSION   DBGJFF010000050 DBGJFF010000000
VERSION     DBGJFF010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26772
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.050"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(57..1364)
                     /locus_tag="ACOIAF_002034"
     CDS             complement(57..1364)
                     /locus_tag="ACOIAF_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HGZ2669044.1"
                     /translation="MRDIRKELVEMLKAEVKPAVGCTEPVALALACAKAKELLGEEIV
                     ENRMLVSPSIYKNGMCVGIPGTERLGLKIAAALGIVGGHSENGLSVLETLTKEEVKIA
                     EDYMDNTPLSITPADTREKVFIEVVLKGKNHIAKVRIRTKHDNFTFLEKDGEVLLDNE
                     PKVSASNDAAEKAESLMDTVTIQELIKNVEGIDFKDIEFLLDGVKMNEEMAEYGLKQK
                     TGIGVGYGIKKSIEEGLLGNDVINYAMMLTAGASDARMAGVKMPVMSSNGSGNHGLTA
                     ILPIVAYNKKFPQSDERLAKALAISHLVTGYIKNYTGRLSAVCGCGVAASTGATAGIS
                     WLMNGTEKQIEGAIENMIADLSGMICDGAKAGCALKLSSAASAAIQSAIIAKQDCFVP
                     PLNGIVGSSVEQSIQNLGRVSDKGMSITDEIILNVMNDMNKVD"
     gene            complement(1789..3564)
                     /locus_tag="ACOIAF_002035"
     CDS             complement(1789..3564)
                     /locus_tag="ACOIAF_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HGZ2669045.1"
                     /translation="MMIMIGLEYIKSDIQNIAEAIVSVLNIDVTIVNKELFRIAGTGV
                     YIDKIGEKVDKYTAFKKSLTEQITILIDDPKSSDICRECYKSGACVEFAEVCCPIICD
                     GVSHGVIGLIAFTKEQAQIIENNKSGLINFLGKMADLISNKLKAQIKTYELELEKKKL
                     ETLLNNMDKAVVSIDVDGKIDKYNSKFKELFNIKDSITGKNIFVELDFIKKPSINNFK
                     KDKSCSFFYKRYGYDSKGIYNINKIVLKNELKGYVIDFIDKRDAIKNYNKMNKDYRIK
                     LENIIGESVAIRNSKKEALIASKSTSTVLITGESGTGKELFARAIHNHSNRVDNPFIA
                     VNCAAIPDNLLESELFGYEEGAFTGAKKGGKLGMFEVAHKGSIFLDEIGDMSLHLQSK
                     LLRVLQEKELNKIGSKSNVSIDVRIIAATNKDLEKMVQNGTFREDLYYRLNVIPIKLP
                     SLRERKEDIPLIIKHMVKEYSKKLDKNVIDIDSRVVDAMMEYKWPGNIRELQNIIEYS
                     VNMSSSSIITMNVIPQKIKNTSVEELEVKSDRIIPLDELEKIEIIKALNKYKDYKKDK
                     ELTAKALGISRATLYRKIEKYNIIS"
     gene            complement(3561..3698)
                     /locus_tag="ACOIAF_002036"
     CDS             complement(3561..3698)
                     /locus_tag="ACOIAF_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669046.1"
                     /translation="MEINYELIIIVMLFVILMSIQFTLNKIYLILKEIRQILMLKKNK
                     E"
     gene            complement(3776..4345)
                     /locus_tag="ACOIAF_002037"
     CDS             complement(3776..4345)
                     /locus_tag="ACOIAF_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HGZ2669047.1"
                     /translation="MKSILCQINKQPVKAKYTSDELECFLDTIEYNVGDKILLVGHIG
                     NLGKRLRGLGTSVTILENSNYEDVCYSLVYNENCNVVKGKLEFLPFDDNQFNKIIIVD
                     QFNVITDCEKASSEIQRVLKGNGEVILEDLNLKNLKVKLNYLKHKFCGSVAKIHYPHE
                     VFNIFSRLNFDGMIKESNKLKYIYVGKRR"
     gene            complement(4529..5164)
                     /locus_tag="ACOIAF_002038"
     CDS             complement(4529..5164)
                     /locus_tag="ACOIAF_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HGZ2669048.1"
                     /translation="MIAVKDENNVVNLRGELDNKLEFSHEIFGEKFYNMKIKINRLSD
                     SFDILPMTVSERLLQDIDLDKQNLVNVVGQLRSYNKTLNNKNRLVLTVFVREIKSIDE
                     ENKDPNSIFLDGYVCKEPVYRKTPLGREITDLLVAINRPYNKSDYIPSIVWGRNAKFA
                     KNLKVGDRIQLWGRVQSREYEKKIDENNVVKKMAYEVSISKIKKLDENNNE"
     gene            complement(5832..6713)
                     /locus_tag="ACOIAF_002039"
     CDS             complement(5832..6713)
                     /locus_tag="ACOIAF_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HGZ2669049.1"
                     /translation="MVAILKALYVVFAAIFAVVFGKDVAKANKEGKLEEQSTPKVAVT
                     GFITDFFDTLGIGSFAPTIAMSKALKLNIPDKKMPGTLNVAHTIPVVTEAFIFTSIIP
                     VDGVTLVSMVVAAAVGSYIGAGIIAKMDERKIQLVMGVTLALTAILMLLGHPWINVLP
                     GGGNELGLTGVKLVIGVIGNFILGALMTAGIGLYAPGMAMVYFLGMSAKVAFPIVMGS
                     CALLMPVASMKFIKEDAYTKKASVIIAICGLIGVFVAAYIVKTLPMDILKALVIVVIA
                     YTSATMIMAANKNKKTA"
     gene            complement(6833..7840)
                     /locus_tag="ACOIAF_002040"
     CDS             complement(6833..7840)
                     /locus_tag="ACOIAF_002040"
                     /EC_number="5.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase"
                     /protein_id="HGZ2669050.1"
                     /translation="MKFSRSIQAIDSHTAGEATRIVVGGIPNIKGNSMPEKKEYLEEN
                     LDYLRTAIMLEPRGHNDMFGSVMTQPCCPDADFGIIFMDGGGYLNMCGHGTIGAMTAA
                     IETGVVPAVEPVTHVVMEAPAGIIRGDVTVVDGKAKEVSFLNVPAFLYKEGVEVDLPG
                     VGTVKFDISFGGSFFAIIHASQLGLKIEPQNAGKLTELAMKLRDIINEKIEIQHPTLA
                     HIKTVDLVEIYDEPTHPEATYKNVVIFGQGQVDRSPCGTGTSAKLATLHAKGELKVGE
                     KFVYESILGTLFKGEIVEETKVADFNAVVPKITGSAYITGFNHFVIDEEDPLKHGFIL
                     K"
     gene            complement(7860..8333)
                     /locus_tag="ACOIAF_002041"
     CDS             complement(7860..8333)
                     /locus_tag="ACOIAF_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HGZ2669051.1"
                     /translation="MGISTSMKNTSLHIFRDPLLDLIDNDSDIDLLGVVVVGTSEHNE
                     WKTFLATRLGRWIESLRPDGVIITLDMAGNQHIDFTNAIAEFVKSDIPTVGLTIMGAD
                     GLVITNPYLDKATIIDYKKTTGYIETEVVGDNHMDEVDVKKALAFLKLKMRKDAK"
     gene            complement(8360..8827)
                     /locus_tag="ACOIAF_002042"
     CDS             complement(8360..8827)
                     /locus_tag="ACOIAF_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HGZ2669052.1"
                     /translation="MGIGPSTKETSLHHFRDPLLDIVESDKDVDLLGVIVVGTPDGNE
                     NKTFVGQRTAAWLEAMRVDGAIVSSDGWGNSHVDYANTFEEIGKRDIPVVGVTFNGTQ
                     AKFVVSNQYMDTIVDMNKSKEGIETEVVGENNTNEIDAKKALAFLKLKMRKHG"
     gene            complement(8842..9600)
                     /gene="prdD"
                     /locus_tag="ACOIAF_002043"
     CDS             complement(8842..9600)
                     /gene="prdD"
                     /locus_tag="ACOIAF_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase cluster protein PrdD"
                     /protein_id="HGZ2669053.1"
                     /translation="MEEKIMRRLVIKPFHMNEVNFGSKTSIKKDVLTIDLSSIDEIKE
                     REELITDIKVDIIKPGDYDREINTIMDIIPISTKVLGRLGEGITHTLTGVYVMLTGAD
                     EDGNQMHEFGSSEGILKDQMVFGRYGTPSVEDYIIHVDVTLKGGLPFERTLPLAAFRA
                     CDDFIQEIRASLKMEDGRNATQVREYFDKIRPNAKKVVIVKQIAGQGAMYDNQLFSKE
                     PSGFEGGTSIIDMGNVPMIISPNEYRDGALRAMT"
     gene            complement(9680..10405)
                     /gene="prdB"
                     /locus_tag="ACOIAF_002044"
     CDS             complement(9680..10405)
                     /gene="prdB"
                     /locus_tag="ACOIAF_002044"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050002 - D-proline reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(9953..9955),aa:Sec)
                     /transl_table=11
                     /product="D-proline reductase (dithiol) protein PrdB"
                     /protein_id="HGZ2669054.1"
                     /translation="MSLTTVQGLQSEIFVPITPPPVWTPVTKELKDMTIALATAAGVH
                     LKSDKRFNLAGDTTFRAIPNTATVDEMMVSHGGYDNGDVNKDINCMFPIDRLHELAAE
                     GFIKGVAPMHYAFMGGGGNQHVFTEETGPAIAAKLKEEGVDGVVMTAGUGTCHRTAVI
                     VQRAIEEAGIPTIIIAALPPVVRQNGTPRAVAPLVPMGANAGEPHNIEMQTHILRDTL
                     EQLVAIPSAGKIVPLPYEYKAHV"
     gene            complement(10443..10727)
                     /locus_tag="ACOIAF_002045"
     CDS             complement(10443..10727)
                     /locus_tag="ACOIAF_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBO2463/CBO2479 domain-containing protein"
                     /protein_id="HGZ2669055.1"
                     /translation="MDRLKYISSETFYEGIIVDIKGGGVTIDLKGRLGQFKIPNRMLI
                     TDYELKIGQEVGFMLSYPEVLSPEPNQEYVENIRREKEKQAEMQSKQEEK"
     gene            complement(10808..12688)
                     /gene="prdA"
                     /locus_tag="ACOIAF_002046"
     CDS             complement(10808..12688)
                     /gene="prdA"
                     /locus_tag="ACOIAF_002046"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050002 - D-proline reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-proline reductase (dithiol) proprotein PrdA"
                     /protein_id="HGZ2669056.1"
                     /translation="MSITLETAQAHANDPAVCCCRFEAGTIIAPENLEDPAIFADLED
                     SGLLTIPENGLTIGQVLGAKLKETLDALSPMTTDNVEGYKAGEAKEEVVEETVEEAAP
                     VSEAAVVPVSTGVAGETVKIHIGEGKNISLEIPLSVAGQAGVAAPVANVAAPVASAAA
                     EVAPKVEEKKLLRSLTKKHFKIDKVEFADETKIEGTTLYIRNAEEICKEANETQELVV
                     DMKLEIITPDKYETYSEAVLDIQPIATKEEGELGSGITRVIDGAVMVLTGTDEDGVQI
                     GEFGSSEGELNTTIMWGRPGAADKGEIFIKGQVTIKAGTNMERPGPLAAHRAFDYVTQ
                     EIREALKKVDNSLVVDEEVIEQYRREGKKKVVVIKEIMGQGAMHDNLILPVEPVGTLG
                     AQPNVDLGNMPVVLSPLEVLDGGIHALTCIGPASKEMSRHYWREPLVIRAMEDEEIDL
                     VGVVFVGSPQVNAEKFYVSKRLGMLVEAMEVDGAVVTTEGFGNNHIDFASHIEQIGMR
                     GIPVVGVSFSAVQGALVVGNKYMTHMVDNNKSKQGIENEILSNNTLAPEDAVRIMAML
                     KNAIEGVEVKAPERKWNPNVKLNNIEAIEKVTGEKIVLEENEQSLPMSKKRREIYEKD
                     EN"
     gene            complement(13117..14877)
                     /gene="prdR"
                     /locus_tag="ACOIAF_002047"
     CDS             complement(13117..14877)
                     /gene="prdR"
                     /locus_tag="ACOIAF_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator
                     PrdR"
                     /protein_id="HGZ2669057.1"
                     /translation="MFSIPEVKKVEEVMDTKFTTIDEDTRIEDAIKEMIKSNTKTLMV
                     IDSSDQLKGIISMTDIHNLYEMHKKYEGQPVKLIMKKDVIYVNEGLTLDECRDIMILK
                     NIGILPVLRDNKIIGVLKQEHIRDYLYMHLEDYGLTLKYIIGQIKEGICAINNEGVVI
                     LWNKFMEERYDIKSEDIVGRPMNEFLENTISEKVLNSKVGMSDLYFTDKKENMYALVH
                     ANPIFYKEEFIGVVCTEVDVTEAKILALELEKVNDTLKYLKNEVKNLSKGSFDKILGK
                     SYKLEKSKAIAKQVARTNSSIFIWGESGTGKEVFARAIHDYSERKGQFIPVNCSAIPN
                     ELFESEFFGYESGAFTGASKKGRIGIFELAKDGTVFLDEIADLPLSMQAKLLRVLQEK
                     EIRRVGGDTTIKINPRIISATNKDLSKMVKAEKFREDLYYRLNVVEIKIPPLRERKED
                     IGLLVHSFLEEICKQNNKPVLTISKDVIDIFQNYRWKGNIRELKNTIENIVVLSQNSK
                     IDVDDVPSYMMDSTNNSTEEEEYPLDLTKATQKIEIKNITKALKMSNGNKAKAAKILN
                     IPRTTLYYKIDQYKIDVSKI"
     gene            complement(15284..17632)
                     /locus_tag="ACOIAF_002048"
     CDS             complement(15284..17632)
                     /locus_tag="ACOIAF_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HGZ2669058.1"
                     /translation="MKTKIKKSSIISLVIAFSMVFTAFTPIVSYADEVTSNDTILNGE
                     EQSNGQTPDVEKPSDGQIPDGEKPSDSQVPDGEKPSDGQMPDGEKPSDSQTPDVEKPS
                     DGQIPDGEKPSDSQVPDGEKPSDGQVPDGEKPSDGQIPDGEKPSDGQIPDGEKPSDGQ
                     IPDGEKPSDGQIPDGEKPSDGQMPDGEKPSDSQTPDVEKPSDGQMPDGEKPLDEQTPE
                     EEKPLEEEIVIEEMSLKQDIDKILDMTLSQINKIVYNFWEDEEDVKADEQSEINQVFT
                     SEDSFISLWYDKKAKVKNSCLLKEIDGSDRPYHDLRFDDITKTLTFDYVIKGLVGASS
                     KDDKYVIEGEDGEQTAFCYNNHLRPPSSNGKSPYLPAEDFNGQENQNEEAVKSILYAG
                     SEFDGFGYKQQFNLGEEENDIMTYSATQSAIWIILGQIDEQERLKQYQGSIDLCDKLI
                     ERAKTEEERAEYQKRKEVAINTKAYLEALLKAGREELKPNDTGKPSLSNGLTKINFEK
                     NEDGTYETEAVALVGYSGVVKLKLPDGVTAYDVDGNIIGTGEVEISTQQKFKLKSVGK
                     PDAKANISAVSYDYIFPKAIQYYKAVLDLGQKDHSSSLPSSKQNLLSYTIEKKNGQEV
                     NFNIGLPTDDDDVVNPPVPPIDDDVVNPPVPPTDDTNGHKPKPSPPIDDTVINPPVPP
                     MDDTIINPPVPPTEDAVLNPPVPPMEDAVLNPPVPPTDDTVLNPPVPPVSDTVEKTPE
                     LSRDDTIVKSPKTGDETQLMSYVFISVIAICGLAYQCKIKRN"
     gene            complement(17787..17915)
                     /locus_tag="ACOIAF_002049"
     CDS             complement(17787..17915)
                     /locus_tag="ACOIAF_002049"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669059.1"
                     /translation="MDNQQVSFEKKKLQRLERNLVPTSVGKWGVPNLWLGNDIVCA"
     regulatory      complement(18213..18298)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            complement(18701..20014)
                     /gene="prdC"
                     /locus_tag="ACOIAF_002050"
     CDS             complement(18701..20014)
                     /gene="prdC"
                     /locus_tag="ACOIAF_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase-associated electron transfer
                     protein PrdC"
                     /protein_id="HGZ2669060.1"
                     /translation="MNKLYSILLKQHVGGPDKPVVSVGDVVKKGTLIAEPTGLGANIY
                     ASVSGKISEINDQAIVIEADEVQEDTFEPLKGEGILDLIKEAGVVGMGGAGFPTHVKL
                     NIDLNGGTILANAAECEPLLAHNIKEIEERPEIVYQGIKYAMEVTNAGKGMLAIKSKH
                     PKAIEAFKKVIKPGDNIEVAELVDMYPMGEERAIVRDVLGKLLEPTQLPSEANAVVIN
                     VETLTRIVEAVEQKRPVISKNITVVGQLNSGKESIVFEDVPIGTTVGELIERAGGIKG
                     EYGEIILGGPFTGKATTLDAPITKTSGGIIVTMPFVNEKRKMGLLVCACGPNEERMRD
                     IATKMGVTDIVSVQKCKQAQEIKGALKCENPGHCPGQAQKCIEFKKAGAEVILIGNCT
                     DCSNTVMGSAPKLKLGVYHITDHVMRTVNHPLIRRIKGDVRLKIK"
     gene            complement(20403..21167)
                     /locus_tag="ACOIAF_002051"
     CDS             complement(20403..21167)
                     /locus_tag="ACOIAF_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HGZ2669061.1"
                     /translation="MKREVKRTKALIYSVLFFSLLLVLIFVFVYGIKYICSKSDNSKM
                     PIYKVDTNEKKIALSFDVAWGTDNMDDILKILDKHNVKATFFLVGSWVDDNEEIVKAI
                     DEKGHEIGNHSNTHANLKEISKEDITKEIETTSEKIYNITGKRTNLFRPPFGDVNNKA
                     MDICSDLGYKVIKWDVDSIDWKELGPNHVIEKVIKESQPGSIVLFHANINDVDNYLDT
                     IISRLKKDGYSLVKISDLLYKDNYVVDSNGVQKTKN"
     gene            21458..21661
                     /locus_tag="ACOIAF_002052"
     CDS             21458..21661
                     /locus_tag="ACOIAF_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HGZ2669062.1"
                     /translation="MSNSNRTVVPEAKAALNQMKLEIANEIGLSNYENIDKGNLTARE
                     NGYVGGYMTKKLVEMAERQMAGK"
     gene            21852..22388
                     /locus_tag="ACOIAF_002053"
     CDS             21852..22388
                     /locus_tag="ACOIAF_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4364 family protein"
                     /protein_id="HGZ2669063.1"
                     /translation="MFENSSEELAYHKLLILYILEKIKMDLTNSQITQVVLETEMMNY
                     FSLQQLLSQLMESKFLTTYKDSDREYYALTQRGVESLEYFFNRIPTSVTEKIDKYIDS
                     NKENLLADTQVKSSFVKQSDNEFIVNLRVIENQSNLIDLNLNVSSEKQAKLICNNWKN
                     NASYMYAEVIDLLIRDIH"
     gene            complement(22392..23258)
                     /locus_tag="ACOIAF_002054"
     CDS             complement(22392..23258)
                     /locus_tag="ACOIAF_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HGZ2669064.1"
                     /translation="MKIYISNYLSKSISIVDYSTLELEKEIVLEDNIYPHHFCIEKEK
                     NLIYIPSSSNGILYVLDLSNDKIIDTVSIGGSLSQVVLSDNELFVANEDSNSIYVLDK
                     NTLNPIGIIGVDNMPHGFDFDRESKKLYVPCINSIVCIDTINKNIQKKINMDFRAWHI
                     ELDKQKKEMYISTLDGKLIIIDEVSMDIKKVLYEFLLPVEIRFNYSGKKVYVADLGYN
                     NVRILDYTTSRYIGNIEINGIPQGLEISKDEKLLFVSDTQENSVKVYETANNKLIKEI
                     KVGKEPTTIICL"
     gene            23406..23651
                     /locus_tag="ACOIAF_002055"
     CDS             23406..23651
                     /locus_tag="ACOIAF_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HGZ2669065.1"
                     /translation="MKVTFNPSGVCCREMIFEVDENNVIVDAEFIGGCNGNLSGLRSL
                     IIGQNALEVADKLNGIDCGGKGTSCPDQLSKAIRQSI"
     gene            complement(23765..25063)
                     /locus_tag="ACOIAF_002056"
     CDS             complement(23765..25063)
                     /locus_tag="ACOIAF_002056"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HGZ2669066.1"
                     /translation="MKYEEALEYISQTNKFGIRLGLENIGKLLELLGNPQETLNIIHV
                     AGTNGKGSVCSFVSNILRECGYKVGLYTSPYLETFTERIRVNGQNIPQDDVARIIELI
                     KEKIEIMVKEGYAYPTEFEVVTAMAFYYYSEQKVDFVALEVGLGGRYDATNIITKSLV
                     SVIVSISLDHTGILGDTIEKIAYEKAGIIKENGVVLVYDQTDEAKDVIKSVCKEKKAK
                     YIEVDFDDINIKKSDINSQIYDCTVMKETYRDLEIKLIGEHQINNSILAISVIKYLKD
                     INKLANINEESIRKGLINTKWPGRIEKIKENPIFIIDGAHNEDGAKSLAKALDKNFKG
                     RKLTLLIGMLEDKDIDSVLEILLPHFNKVITTTPSNPRAINSDILREKVLKYVDDVTS
                     KHEIEDAVNYTLETSSEDDIIISAGSLYMIGTVRTLVKKL"
     gene            complement(25196..26209)
                     /locus_tag="ACOIAF_002057"
     CDS             complement(25196..26209)
                     /locus_tag="ACOIAF_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HGZ2669067.1"
                     /translation="MNNKVKVAVLDTGIDKEHDYLKDNLVGGIAFECMHDYIFISDKF
                     DDEDGHGTACASIIKKEYEDVELFVIKILRKDGITNIKVLEEALRYLLDTNIRLINLS
                     LSVIGVESVKGLFEVCYELFRKGKIIVCSLANDFDLSYPAMFNNVIGVRGFTLDGDDS
                     FWYNKKYDVQCVMDSNSYISCDINNSYRLPPKCNSYLSAKLTGKIAKILSEKPGITIS
                     DLNDKLESLATRNHWDSCDFDECSRIVNFKLDLYDKKNALLMKVADVVRDCLDIEDNN
                     EKLFKGSLFNKEIGLVYDNCFNLLKKLEHRFDIKFNYMDISKYDLVSIYTLTELVERY
                     KNL"
BASE COUNT         8092 a         4909 c         3008 g        10763 t
ORIGIN      
        1 aaatttattt atgctatttt tgttatacct cataatacaa aacatattat aaagtactag
       61 tcaactttat tcatatcatt cattacattt aagattatct catctgttat agacatacct
      121 ttgtcactta ctctacctaa gttttgtata ctttgctcaa cacttgaacc aactatacca
      181 tttagtggtg gtacaaaaca atcttgctta gctattatag cactttgtat agctgcagat
      241 gctgcagaag ataattttaa tgcacaacca gctttcgcac cgtcacatat cattccactt
      301 aagtcagcta tcatattttc tatagctcct tctatttgct tttcagtacc attcataagc
      361 caagagatac cagcagttgc tccagtagaa gcagcaacac cacatccaca tacagcagat
      421 aatcttcctg tatagttctt aatataacca gttactagat gagatatagc taaagctttt
      481 gctagtcttt catcagattg tgggaatttt ttattataag caacgattgg aagaattgct
      541 gttaatccat gatttccact tccatttgaa ctcataacag gcatttttac ccctgccatt
      601 cttgcatctg aagctccagc agttaacatc attgcataat ttataacatc attacctaaa
      661 agaccttctt ctatagactt tttaattcca tatcctacac caataccagt tttttgtttt
      721 aatccatatt cagccatctc ttcattcatt tttactccat ctagtaaaaa ttctatatct
      781 ttaaaatcaa ttccttctac attttttatt aattcttgta tagttactgt atccattaaa
      841 ctttcagctt tttctgccgc atcatttgaa gctgatactt ttggttcatt gtctaataat
      901 acttctccat ctttttctaa gaacgtaaaa ttatcatgtt tagttcttat tctcacttta
      961 gcaatatgat tttttccttt taacacaact tctataaata ctttctctct tgtatcagca
     1021 ggtgttattg atagtggagt attatccata taatcctcag ctattttaac ttcctctttt
     1081 gttagagttt ctaacacact taatccattt tcagaatgtc cacctactat acctagagct
     1141 gctgcaattt ttaatcctaa tctttcagta cctggtatac ctacgcacat accattttta
     1201 taaatactag gactaactaa catacgattt tcaactattt cttctcctaa taattccttt
     1261 gcctttgcac atgctagagc taaggctact ggttcagtac accctactgc tggtttaact
     1321 tctgccttta acatttccac taattctttt cttatatctc tcattatata taaacctccc
     1381 aattaagttt agatttagta ctcaaataat taattaaata atttcttatc tatatttata
     1441 gcaaaataca tgccatattt tttatatctt tttatatttt tagtcttata ttctataacc
     1501 attggtatga ctgcttctat ttatgtattt tttttattta catagagtat ttatattctt
     1561 taaaatggag aaacatatga caatttcgca aaaagacaaa aaaaagtctc aaaatgagat
     1621 tctatatcat tttgagacgg ctcaaatgta ttatttttta acttataaaa accatttttt
     1681 aattcataaa aaaagattct gcaaaacact tatagtataa gtttaaagct ttctttaaag
     1741 ttatgaattt agaaaaattt aaattttata tagcttttta ttatattact atgaaattat
     1801 attgtatttt tcaatttttc tatataatgt agctcttgat attccaagtg ctttagctgt
     1861 taattcttta tcttttttat aatctttgta cttatttaaa gcttttatta tctcaatctt
     1921 ctctagttca tcaagtggaa ttatcctatc gcttttaact tctaactctt ctacagaggt
     1981 attcttaatt ttttgaggaa ttacattcat tgtgattatt gaagaagaag acatattcac
     2041 actatattca ataatattct gcaactctct tatattccct ggccacttat attccatcat
     2101 agcatcaaca accctgctat ctatgtcaat aacattttta tctaattttt ttgaatactc
     2161 tttaaccata tgcttgatta taagtgggat atcttctttt ctctctctta aacttggtaa
     2221 ttttataggt ataacattta atctatagta taaatcttct ctaaaagtgc cattttgaac
     2281 cattttttct aaatctttat ttgtagcagc aataattctt acatctattg aaacatttga
     2341 tttacttcca attttattaa gttccttttc ttgtaatact ctcaataact ttgactgtaa
     2401 atgcaaactc atatctccta tttcatctaa aaatatactt cccttatgag ctacttcaaa
     2461 cattccaagc tttccacctt ttttagctcc agtaaaagct ccctcttcat atccaaataa
     2521 ttcactctct aataaattat ccggtatagc agcacagttt acagctataa aaggattatc
     2581 tactctattg ctatggttgt gtatggctct tgcaaatagc tccttacccg ttccactttc
     2641 cccagtaata agtactgttg atgttgattt agaagctatt agggcttcct ttttagaatt
     2701 tcttatagct acactttctc caattatgtt ttccaatttt attctataat ctttattcat
     2761 cttattgtag ttttttatag catctctttt atctataaaa tcaataacat atccctttaa
     2821 ctcatttttt aatactattt tattgatgtt gtatattccc ttcgaatcat atccgtatct
     2881 tttataaaaa aaactacagg atttgtcctt cttaaagtta tttatagaag gtttctttat
     2941 aaaatctaat tcgacaaata tgtttttgcc agttatacta tcctttatat tgaaaagctc
     3001 tttaaatttt gaattatatt tatcaatttt tccatccaca tcaatagaca caacagcttt
     3061 gtccatattg tttaacagtg tctctaattt tttcttttct aattcaagct cataagtttt
     3121 aatttgagct tttaatttat tagatattaa gtctgccatt ttgcctaaaa agtttataag
     3181 accactttta ttattttcta taatctgtgc ttgttccttt gtaaaggcta taaggcctat
     3241 aacaccatga gagactccat cacatataat gggacaacac acctctgcaa actctacaca
     3301 agccccactt ttatagcatt ccctacatat atcactactc tttgggtcat caattagtat
     3361 tgtaatctgc tctgtaagtg attttttaaa agccgtatat ttatctactt tctcacctat
     3421 tttgtctata tatactcctg tacctgcaat cctaaataac tccttattta ctatagttac
     3481 atctatattg agtactgata ctatagcttc agctatattt tgaatatcag attttatata
     3541 ttctaaacct atcataatca tcattcctta tttttcttta gcataagtat ttgtcttatt
     3601 tctttaagaa tcaagtatat cttatttaaa gtaaattgga ttgacatcag gattacaaag
     3661 agcataacaa ttattattaa ctcataattt atttccatct aaacatctcc ttataacaaa
     3721 agactaggct taacctagtc ttttatatta tatccatatt tatattttaa ctcatttatc
     3781 ttctttttcc cacataaata tattttaatt tatttgactc tttaatcata ccatcaaagt
     3841 ttaatcttga aaatatatta aatacttcat gtggatagtg aatctttgca acagaaccac
     3901 aaaatttatg tttaagatag tttaacttaa cttttaaatt ttttaaattt aaatcctcta
     3961 atataacctc tccattgccc tttaacactc tttgtatttc agatgaagct ttttcacaat
     4021 ctgtaataac attaaattgg tcaactatta ttatcttgtt gaactgatta tcatcaaatg
     4081 gtagaaactc taacttacct ttaactacat tgcaattttc attatacact agagaataac
     4141 agacatcttc ataattacta ttctccagta tagttacaga agttccaaga cctctcaatc
     4201 tcttgcctag gtttcctata tgaccaacaa gcaatatttt atcacctaca ttatactcaa
     4261 ttgtatccaa aaaacattct aattcatcag atgtatattt agctttaacc ggctgtttat
     4321 ttatctgaca taatatgctt ttcacaatgc ttcacctctt catttcctca attataaatt
     4381 atgtacagat tacattataa taaatggcga gcaactattc aagagatatt tcattagtat
     4441 ttttttaaac aatctttata cattttaata tgatattata ccaaatttat taggatttta
     4501 ttcataaaaa tcagtctatt ttttatgttt attcattgtt attttcatcc aattttttta
     4561 ttttcgatat ggatacttca tatgccattt ttttgaccac attattttca tctatttttt
     4621 tctcatattc tctactttga accctacccc aaagctgtat tctatcacca acctttagat
     4681 tctttgcaaa tttagcattt ctaccccaaa ctattgatgg tatataatca gatttattat
     4741 aaggtctgtt tatagcaact aataaatcgg ttatttccct tcctaaaggt gtcttcctat
     4801 acacaggttc cttacataca tatccatcta aaaatatact attagggtct ttattttcct
     4861 cgtctatgct ttttatttct ctgacaaaca ctgtaagcac taatctattt ttattattta
     4921 atgttttgtt atatgatctt aattggccta cgacattgac taaattttgt ttatcaaggt
     4981 caatatcttg gagaagtctt tctgatacag tcattggaag tatatcaaaa gaatcgctta
     5041 gtctgtttat ttttattttc atattataaa atttttctcc aaatatctca tggctaaatt
     5101 ctagcttatt gtctaactcc cctcttaagt ttactacgtt attctcatct ttaacagcta
     5161 tcatatttta tccctccaca attgttaatt cttatatttc tggtatcagc ttatataata
     5221 aatatattta taagggatgt attttattac aaatctttga ttgctgttta aaagttttta
     5281 tttagttata aatttcattt aatatttcac ttttataatt ataaaattat tatctttttg
     5341 taccacatat actaattaaa ctacctattt cagttttgta ttaattataa aaatcctatt
     5401 atataattaa taagtcatat ataacttaaa tttattcact ctatataagc aacttcaacg
     5461 aattaaattt attcaaataa ttcagatgaa tattgagtca ttataaactt atttttctta
     5521 attataagtt atattagact tattacaggt tatattttta ttaatctgta ttaatatatg
     5581 aacatttatt gaacttattg attataaaat ttaaatattt agctttaaat tatgctttat
     5641 tctttaaaaa tacaacgttt tttacattaa atctactaaa tcactatata tattacataa
     5701 ttggcatgtt aattgcttta tattaattta gtaaataaaa aactgcccta aaatattaat
     5761 tcaatatttt aaagcagttt ataatcttta ttattagctg taatatttaa taaacactaa
     5821 gtagtcttta actaagcagt ctttttgttt ttatttgcag ccattatcat tgtagcagaa
     5881 gtataagcta taactactat tactaatgct tttaatatat ccattggtag agtttttact
     5941 atatatgcag ctacgaaaac tcctattaaa ccacatatag ctattataac tgatgctttt
     6001 ttagtatatg catcttcttt tatgaatttc atacttgcaa ctggcattaa taatgcacaa
     6061 gaacccataa ctattgggaa tgcaactttt gcagacattc ctaagaaata aaccattgcc
     6121 atacctggag catatagacc tataccagct gtcattaaag ctcctagtat aaagttacct
     6181 attacaccta ttactaattt aacaccagta agacctaatt catttcctcc acctggtaat
     6241 acgttaatcc aaggatgacc taataacatt aatattgctg ttaatgctaa agttacaccc
     6301 ataacaagtt gtatttttct ctcgtccatc ttagctatta ttcctgcacc tatatatgaa
     6361 ccaactgctg ctgccacaac catagatact aaagtaacac catcaactgg tatgattgat
     6421 gtaaatatga atgcttctgt aacaactgga atagtatgtg ctacgtttaa tgtaccaggc
     6481 atttttttat ctggtatatt taactttaaa gcctttgaca ttgctattgt tggagcaaat
     6541 gaccctattc ctaatgtatc aaagaagtct gttataaatc ctgttacagc aacttttgga
     6601 gtactttgtt cttctagttt cccctctttg tttgctttag ctacgtcttt accaaacaca
     6661 actgcaaaga ttgctgcgaa tactacatat aatgccttta agattgctac cataatccat
     6721 cctcctattt ttttattttt tcatatattc tgaatcttat agcctaaaag ctatagaata
     6781 gcaaccgaca agtggctact attctatttt aatttcctaa tttatttatt acttatttaa
     6841 gaataaatcc atgtttaagt gggtcttctt catctattac aaaatgatta aatccagtta
     6901 tataagcaga accagttatt ttaggtacta cagcattgaa atctgcaact ttagtttctt
     6961 caactatttc acctttgaat aaagttccta atatactttc atatacgaat ttttctccta
     7021 cttttaattc acctttagca tgtaaagtag ctaatttagc acttgttcca gttccacatg
     7081 gagatctgtc aacttgacct tgaccaaata taactacatt tttgtaagta gcttctggat
     7141 gagttggctc atcatatatt tcaactaagt ctacagtttt tatatgagct aaagttggat
     7201 gttgtatttc tattttctca tttattatat ctctaagttt catagctagt tcagttaatt
     7261 taccagcatt ttgaggttcg atttttaaac ctaattgact tgcatgtatt atagcaaaga
     7321 agcttccacc aaatgatatg tcaaatttaa cagttccaac acctggtaag tcaacttcaa
     7381 caccttcttt atataagaaa gctggtacgt ttaagaatga tacttcttta gcttttccat
     7441 ctacaactgt aacgtctcct cttattattc ctgctggagc ttccataact acatgagtta
     7501 caggctctac tgctggaact acacctgttt ctatagctgc tgtcatagct cctattgtac
     7561 catggccaca catgttaagg tatccgccac catccatgaa gattattcca aagtcagcat
     7621 ctgggcaaca aggttgagtc attacagaac caaacatatc attatgtcct cttggctcta
     7681 acattatagc agttcttaaa taatctagat tttcctctaa atattctttt ttctcaggca
     7741 tagaatttcc ttttatgtta ggtattccac ctacaactat tctagttgct tcacctgctg
     7801 tatgagagtc tatagcttgt atacttctgc taaatttcat atcaaattcc cccttaggtt
     7861 tattttgcgt ctttacgcat ttttaatttt aggaaagcca atgctttttt aacatcaact
     7921 tcgtccatgt gattgtctcc aactacttct gtttctatgt agcctgttgt ctttttataa
     7981 tcaattatgg tagctttatc caaatatgga ttggtaatta ctaatccatc tgctcccata
     8041 atagtaagac ctactgtagg tatatctgat tttacaaact ctgctatagc atttgtaaaa
     8101 tcaatgtgct ggtttcctgc catatctaaa gttataatca ctccatcagg tcgcaatgat
     8161 tcaatccatc ttccaagtct agttgctaaa aatgttttcc attcattgtg ctctgatgtc
     8221 ccaactacaa ccactcctaa caagtctatg tcagaatcat tatctatcaa atctagtaac
     8281 gggtctctaa atatatgaag tgatgtgttt ttcattgatg tagaaattcc cacaaaactc
     8341 attcccttac ttaaattttt tatccatgtt ttctcatttt aagttttaaa aatgccaaag
     8401 cttttttagc atctatttca tttgtgttgt tttctccaac tacttctgtc tcaatacctt
     8461 cttttgattt gttcatatca actatggtat ccatatattg attgcttaca acaaacttag
     8521 cttgagtccc attaaatgta actccaacta caggtatatc tctttttcct atttcctcaa
     8581 atgtgttagc gtagtcaaca tgtgagtttc cccaaccatc tgatgaaact atagctccat
     8641 ctactctcat ggcttccagc caagcggccg ttctttgtcc cacaaaggtt ttattttcat
     8701 ttccatctgg agttcctact actattactc caagtaaatc tacatcctta tcactttcaa
     8761 ctatgtctaa aagtgggtct ctaaagtgat gaagagatgt ttcttttgtt gaaggtccta
     8821 ttcccataat atccacctct cttaagtcat agctctaagg gcaccatctc tgtattcatt
     8881 aggagatata atcataggta catttcccat atctataatt gatgttcctc cttcaaatcc
     8941 actaggttcc tttgagaata actgattgtc atacatagca ccttgacctg ctatttgttt
     9001 tactatgaca accttttttg catttggtcg aattttgtca aaatattcac gaacttgagt
     9061 agcatttctt ccgtcttcca ttttcaaaga cgctcttatt tcttgtataa aatcatcaca
     9121 tgctctgaat gctgcaagtg gaagtgttct ttcaaaaggt aaacctccct ttaaagttac
     9181 atctacatgg attatataat cttcaactga tggagttcca tatctcccaa ataccatttg
     9241 gtctttcaaa attccttctg aagaaccaaa ttcatgcatt tgatttccat cttcatctgc
     9301 accagttaac attacatata cacccgttaa agtatgtgta ataccctctc ccagtctgcc
     9361 taaaactttt gttgatattg gtataatgtc cataatagta ttaatttctc tgtcataatc
     9421 tccaggtttt ataatgtcta cttttatgtc tgttattaac tcttctcttt cttttatttc
     9481 atctatactt gataaatcta ttgtaagtac atctttcttt atagaagttt ttgaaccgaa
     9541 attaacttca ttcatatgaa acggttttat aacaagtctt ctcattattt tttcttccat
     9601 aatatccacc tttgttctta aaacccctct cctaaccgtg taggagaagg gttctatatt
     9661 ttcaaatata aattacattt taaacgtgag ctttatattc gtatggtaat ggaactatct
     9721 taccagcaga tggtattgca actaattgct ctaatgtatc tcttaatata tgtgtttgca
     9781 tttctatatt atgtggttca ccagcattag cacccattgg aactaatgga gcaactgctc
     9841 taggagttcc gttttgtcta actactggag gaagagctgc tattattatt gtaggtattc
     9901 cagcttcctc tattgctctc tgcacgatca cggcagttct atggcaagta cctcagccag
     9961 ctgtcataac tacaccgtct actccctctt ccttaagttt agcagcgata gcaggaccag
    10021 tttcttcagt gaagacatgt tggtttcctc caccacccat gaatgcatag tgcataggag
    10081 ctactccttt gataaatcct tctgcagcta attcatgtaa tctatctata gggaacatac
    10141 agtttatatc tttgtttaca tctccattgt catatccacc atgtgatacc atcatttcat
    10201 caactgtagc tgtattaggt atagctctaa atgtagtatc tcctgctaga ttaaatctct
    10261 tgtcagattt taaatgaaca ccagctgctg tagctaaagc tatagtcata tcttttaatt
    10321 ctttagttac aggagtccaa actggaggag gtgttattgg aacgaatatt tcagattgaa
    10381 gtccttgtac tgttgtaagg ctcatatatt tcctctccct tctattttca aacgattatt
    10441 tattattttt cttcttgttt tgattgcatt tctgcttgtt tttctttttc tctcctaata
    10501 ttttctacat actcttgatt aggctctgga cttaatacct caggatagct tagcatgaat
    10561 ccaacttctt gacctatctt aagttcgtag tcagttataa gcattctatt aggtatttta
    10621 aattgtccaa gtctaccttt taggtctata gtcactccac cgcctttgat gtcaactata
    10681 ataccttcat aaaaagtctc tgatgagata tattttaacc tatccattat aattctacct
    10741 ctctatactg ttttaaactt ggatctaaat ttatgttatt gtctaaatct tagttagtat
    10801 ctaaatttta gttttcgtct ttttcgtata tttctcttct cttcttactc attggtagag
    10861 attgctcatt ctcttctaat actatttttt ctcctgtaac tttttctata gcttctatgt
    10921 tatttaattt aacatttgga ttccattttc tttcaggagc tttaacttct acaccttcta
    10981 tagcattttt aagcatagcc attattctaa cagcatcttc tggagctaaa gtgttgttag
    11041 ataatatttc attctctata ccttgcttag acttattgtt gtctaccatg tgagtcatgt
    11101 atttattacc aacaactaga gcaccttgaa cagctgagaa acttacacca actactggta
    11161 tacctctcat acctatttgc tctatgtgag atgcgaaatc tatatggttg tttccgaaac
    11221 cttcagtagt tactacagct ccatcaactt ccatagcttc aactaacata cctaatctct
    11281 tagatacata gaatttctca gcatttactt gtggagaacc aacaaataca acacctacta
    11341 aatctatttc ttcgtcttcc atagctctta ttactaatgg ctctctccag taatgtcttg
    11401 acatttcttt tgatgcaggt cctatacaag ttaatgcatg gataccacca tctaatactt
    11461 caagtggaga taatacaact ggcatgtttc ctaagtcaac gtttggttga gctcctaatg
    11521 taccaactgg ctcaactggt aatattaggt tatcatgcat tgcaccttgt cccattattt
    11581 cttttataac aacaactttc tttttacctt ctcttctgta ttgctctatt acttcttcat
    11641 caactactaa agagttgtca actttcttta atgcttctct tatttcttga gttacatagt
    11701 caaatgcacg gtgagcagct aaaggtcctg gtctttccat gttagttcct gcttttattg
    11761 ttacttgacc tttgatgaat atttcacctt tgtcagcagc acctggtcta ccccacatta
    11821 tagtagtatt taactcacct tctgaagaac caaattcacc tatttgaact ccatcttcat
    11881 ctgtaccagt taataccatt acagctccat ctataactct agttatacct gaacctaatt
    11941 cgccttcttc tttagtagcg attggttgta tatctaatac agcttcactg taagtttcat
    12001 atttatcagg agttattatt tctaacttca tatctacaac taactcttga gtttcattag
    12061 cttctttaca tatttcttct gcgtttctga tgtataaagt agttccttct attttagttt
    12121 catcagcaaa ttcaacttta tctattttaa agtgtttttt agttaagctt cttaaaagtt
    12181 tcttttcttc aacttttgga gctacttctg ctgctgcact tgccactgga gcagcaacgt
    12241 ttgctactgg agcagcaact cctgcttgac cagctactga taaaggtatc tctaagctta
    12301 tgttcttacc ttcacctatg tgtattttaa ctgtttcacc tgcaactcct gtgcttactg
    12361 gaactactgc tgcttctgat actggagctg cttcttctac tgtttcttct actacttctt
    12421 ctttagcctc tcctgctttg tatccttcta cgttatctgt agtcattgga gaaagtgcat
    12481 ctaaagtttc ttttaactta gctcctagta cttgacctat agttaatcca ttttctggta
    12541 ttgttaataa tccagaatcc tctaagtctg caaatattgc tggatcttct aagttttctg
    12601 gcgctataat tgttcccgct tcaaatctac aacaacaaac tgctgggtca tttgcatggg
    12661 cttgagctgt ttctaaagtt attgacatag tataaaatac ctccttacat tattttctta
    12721 tttattattt taaatctcaa ttcctcaaca ggtaacccct taactgagaa attaagtaaa
    12781 ttaaaaccta cataatttgt tatgattaat attatttttt tctcacttaa agtcacttta
    12841 tattataagc aattcctatg ccaacttatt aacaaaaaga ttttttctat aatagattct
    12901 tcatctatcg ctatttttag ccaaatatta tataaatatt tatttaaaat atgttatttt
    12961 ttgtatttgt aaacattttc tcaaacaaaa aaagtgtcaa ttaatcaaca ctttttcatt
    13021 ttttttaaca catctatata cacaacactt atacaagcgt tggctttact aggtttcaaa
    13081 ttaaagttta tcattttgtc aaaatttagt catgtgtcat attttagaca catctatttt
    13141 gtattggtct atcttataat acaaagttgt tctgggtata tttaatatct tagctgcttt
    13201 agctttattt ccattagaca ttttaagtgc cttggttata ttctttattt caattttttg
    13261 agttgccttc gttaaatcta aaggatactc ttcttcttca gtactattat ttgtagaatc
    13321 catcatataa cttggtacat catccacatc tattttagaa ttttgtgaca atacaacaat
    13381 attttcaatc gtatttttta attctctaat attacctttc catctataat tctgaaatat
    13441 atctattaca tctttgctaa ttgtaagaac aggcttatta ttttgtttac atatctcctc
    13501 taaaaagcta tgaaccaata atcctatgtc ttcttttctc tctctaagtg gaggaatctt
    13561 tatttcaaca acatttaatc tatagtataa atcttctcta aatttttctg cctttaccat
    13621 tttactcaaa tctttattgg tagctgatat tattctagga tttattttta ttgtggtatc
    13681 tccaccaact cttcttattt ctttttcctg taatactcta agcaactttg cttgcatact
    13741 aagaggtaaa tcggcaattt catctaaaaa tacagttcca tcttttgcaa gttcaaatat
    13801 acctattcga ccctttttac ttgctcctgt aaatgcacct gattcatacc caaaaaactc
    13861 actttcaaat aactcatttg gtatggcact acagttaaca ggtataaatt gcccttttct
    13921 ctcactataa tcatgaatag ctcttgcaaa tacttcttta cctgtaccac tttcgcccca
    13981 aataaatata ctactatttg ttctagccac ttgttttgca atcgctttcg atttttccaa
    14041 tttataactt ttacctaaaa tcttatcaaa actaccttta gataaatttt tgacttcgtt
    14101 ctttagatat tttaatgtat catttacttt ttcaagttca agagctaata tcttagcttc
    14161 tgtaacatca acttctgtac atacaacacc gataaattct tctttataaa atataggatt
    14221 tgcatgaact aatgcataca tattttcttt tttatctgta aaatacaagt cactcattcc
    14281 aactttacta tttagtactt tttctgaaat agtattttcc aaaaattcat tcatcggtcg
    14341 accaactata tcttcagact ttatatcata cctttcctcc ataaacttat tccacaagat
    14401 aacaacacct tcgttattta tagcacaaat tccttcttta atttgaccta taatatactt
    14461 caaagtcaat ccataatctt ctaaatgcat atatagatag tccctaatgt gctcttgttt
    14521 tagaactcca ataattttat tatctctaag aacaggaaga atccctatat tttttaatat
    14581 cattatatct ctacattcat ctaaggttaa accttcgttg acatatatta catccttctt
    14641 cattataagt tttactggtt gaccttcata ctttttatgc atctcataaa gattatgtat
    14701 atccgtcatg gatataatgc cttttaattg gtcagatgaa tctattacca ttagagtttt
    14761 tgtgttgctt tttatcattt cttttattgc atcttcaatt cttgtgtctt cgtctatagt
    14821 tgtaaatttt gtatccatta cctcttctac tttttttact tctggtatcg aaaacataaa
    14881 gtcccccctt tatagtagta cctcatcaaa acttatttat atatatttat tctagcatat
    14941 tataataatt atttattaaa taattattat taaaatttat taagtaatta gcatttaaat
    15001 ttatataatc actttaaaat ttataattaa atttatacaa atgaaatcaa atgaattata
    15061 cccttagtat attatttttt caatactatt ttcaccttca aaacattttt ctattcatca
    15121 aacaattaat gaatgcctgt atccaatcat aaatttaaga atataattaa aatagatatt
    15181 tttaaacaaa ctatatttgc atattatatt tacataaaaa agccccaaaa tgacttataa
    15241 atcaatttga gactttttat tttaaactta ataataatct agattaattt ctttttattt
    15301 tacattgata cgctaatcca caaattgcaa tgacactaat aaatacatat gacattagct
    15361 gagtttcatc acctgttttt ggtgatttta ctattgtatc atctcttgat agttctggag
    15421 ttttttccac tgtatcactt actggtggta ctggtggatt taacactgta tcatctgttg
    15481 gtggcactgg tggatttagc actgcatcct ccattggtgg cactggtgga tttaacactg
    15541 catcctccgt tggtggtact ggtggattta ttattgtatc atccattggt ggtactggtg
    15601 gatttattac tgtatcatct attggtggtg atggttttgg cttatgtcca tttgtatcat
    15661 ctgttggtgg caccggtgga tttacaacat catcatctat tggtggcact ggtggattta
    15721 caacatcatc atcatctgtt ggtaaaccta tattaaagtt aacttcttga ccattctttt
    15781 tctctattgt ataacttaat aaattttgct tgctagatgg taatgaagat gaatggtctt
    15841 tttgtcctaa atcaagcact gccttataat attgaatagc ttttggaaaa atataatcat
    15901 aagatacagc agaaatatta gctttggcat ctggttttcc aacactctta agtttaaatt
    15961 tctgttgcgt tgaaatttct acttcgcctg tacctattat attaccatct acatcataag
    16021 cagttactcc atctggtagt tttaacttta ctacaccact ataacctact aaggctacag
    16081 cttctgtttc ataagtacca tcttcatttt tttcaaagtt aatcttagtt aaaccattac
    16141 tcaaactagg ttttcctgta tcatttggct ttaactcttc acgacctgct tttaacaatg
    16201 cttctaaata agcttttgta tttatcgcta cttctttcct cttctgatac tcagctcttt
    16261 cctcttcagt ttttgctctt tctataagtt tatcacaaag gtctatagag ccttgatact
    16321 gcttaagtct ttcttgttca tctatttgtc caagaataat ccaaattgca ctttgagtag
    16381 ctgagtaagt cattatgtca ttttcttctt cgcctaaatt aaattgttgc ttatatccaa
    16441 atccatcaaa ttcagaacct gcatatagta tagatttaac tgcttcttca ttttggtttt
    16501 cttgaccatt aaaatcttct gctggtaaat atggactctt tccattacta cttggtggtc
    16561 ttaaatgatt attgtaacaa aatgcagttt gctctccatc ttcaccttct attacatatt
    16621 tgtcgtcttt tgagcttgct cctacaagac cctttattac atagtcaaaa gttaaagtct
    16681 ttgtaatatc atcaaatctt aaatcatgat aaggtctatc tgaaccatct atttctttta
    16741 gtaaacaaga atttttcacc tttgcttttt tgtcatacca taagctaata aatgagtctt
    16801 ctgaagtaaa aacttggtta atttctgatt gttcatctgc ctttacatct tcttcatctt
    16861 cccaaaagtt atatactatt ttatttattt gagataaagt catatcaaga attttatcaa
    16921 tatcttgttt taaactcatt tcctctataa ctatttcttc ttccaatggt ttttcttctt
    16981 ctggagtttg ctcatctaat ggtttctccc catctggcat ctgaccatct gatggtttct
    17041 ctacatctgg cgtctgacta tctgatggtt tctctccatc tggcatctga ccatctgatg
    17101 gtttctcccc atctggtatc tgaccatctg atggtttctc tccatctggt atctgaccat
    17161 ctgatggttt ctctccatct ggtatctgac catctgatgg tttctctcca tctggtatct
    17221 gaccatctga tggtttctcc ccatctggta cctgaccgtc tgatggtttc tccccatctg
    17281 gtacttgact gtctgatggt ttctccccat ctggtatctg accatctgat ggtttctcta
    17341 catctggcgt ctgactatct gatggtttct ctccatctgg catctgacca tctgatggtt
    17401 tctccccatc tggtacttga ctgtctgatg gtttctcccc atctggtatc tgaccgtctg
    17461 atggtttctc tacatctggt gtctgaccat ttgattgttc ttctccatta agtattgtat
    17521 cattgctagt cacttcgtca gcataagata ctattggtgt aaatgcagta aataccatac
    17581 taaaagcaat tactaaactg attatacttg attttttaat ttttgttttc aaaattctcc
    17641 tgatactaaa aacatatatt gtattcactt aatataatta aaattattaa atattatatg
    17701 aatcttattc aataatacat ctttagtgtg ttcaattagg gtcgttactc ctaaccagtt
    17761 ctcctatgaa cttcttaata tttctattaa gcacagacta tatcattacc taaccataaa
    17821 ttaggtactc cccatttccc cacacttgta ggtacgagat ttctctctag tcgttgaagt
    17881 ttcttctttt cgaagcttac ctgctgatta tccattttaa ggcacttagg atttaacctt
    17941 atgctatcta actaattttt tctactttcg taacctcaag tgcttattaa atgcacactc
    18001 tatcacactt atccttattt catgattatg ttgtggttta gttagcttta ggaacttcca
    18061 gcaattaaag gagttttggg cagatatctg ccactctaca ctcgttatga atgcagggag
    18121 catttcaaaa atatatttat atctatcttc tattaattac ttatttatgt atttatttta
    18181 tatatttttg acaattaaaa gttactccta tttatattta tagcaggttg cactactaac
    18241 tccatatatc ttttaagtgc ccaggtttaa gatatacttc acagtttcta taaataggtt
    18301 ttttaatttt tcttttttaa ctttttaagt gcactttttt aacaactcaa taaaatataa
    18361 attagtatcc tagatactta tctaataaaa gacaaaaatt agtttttata ataaaaaaga
    18421 tactatagag tatcttcaaa tatcacaaaa accttttact ctatcggata gttgacttaa
    18481 tataatatgc atattaatac aaaccacata cttgagtatc attatttttg tccaatatta
    18541 attgtaatag ctattgttat cattatacaa tatctagcat attttgtcta ttttttccat
    18601 ttattttaca atatatattg ccctcaaaat acattaaaaa acatctttag aagtaacttt
    18661 taagttttac ttctaaagat gtctaacttc tatttatcta ttattttatt tttaatctta
    18721 cgtcaccttt tattcttctt attaatgggt gattaactgt tctcattaca tggtcagtaa
    18781 tatgatatac acctaatttt aattttggtg cagaacccat tactgtgtta ctacagtcag
    18841 tacagtttcc tattaatata acttctgctc cagctttctt aaattcaata catttttgag
    18901 cttgtcctgg acagtgtcct ggattttcac attttaatgc acctttaatt tcttgagctt
    18961 gtttacattt ttgaactgat actatatcag taactcccat tttagttgct atatctctca
    19021 ttctttcttc attaggtcca catgcacaaa ctaaaagacc catttttctc ttttcattaa
    19081 caaatggcat agtaactatt ataccaccac tagttttagt tattggagca tctaaagtag
    19141 tagctttacc agtgaaaggt cctcctagga ttatttcacc atattcacct tttattccac
    19201 cagctctttc aattaattct ccgacagttg ttccaattgg cacatcttca aacactatag
    19261 attcttttcc actgtttaat tgaccaacta cagttatatt tttagatata actggtcttt
    19321 tttgctcaac agcctctact attctagtta gtgtttctac gtttattact actgcattag
    19381 cctctgaagg taattgagtt ggctcaagta attttcctag aacgtctctt actattgctc
    19441 tctcttctcc cattgggtac atatcaacta gttctgcaac ttctatgttg tctcctggct
    19501 ttattacttt tttgaaagct tctattgctt ttggatgctt actttttatt gctagcatac
    19561 cttttccagc attagtaact tccatagcat attttatacc ttgataaact atttctggtc
    19621 tttcttctat ttcttttata ttatgagcta ataaaggttc acattcagct gcatttgcta
    19681 atattgttcc accatttaaa tctatgttta attttacatg agtagggaat cctgctccac
    19741 ccattcctac tactccagct tctttaatta agtcaagtat accttctcct tttaatggtt
    19801 caaaagtatc ttcttgtact tcatcagctt caattactat cgcttggtca tttatttcac
    19861 ttatttttcc actaacactg gcataaatat ttgcccctaa tccagttggt tctgcgatta
    19921 gagttccttt ttttactaca tctccaacac ttactactgg cttatctgga ccaccaacgt
    19981 gttgttttaa taaaatagaa tatagtttgt tcaactagta cgccccctta agtatattaa
    20041 tttttttata agctgttcaa ataatgttaa tgctattttt ctattataag gtcttaacat
    20101 attgtttatt tctatagaac atatcgctta ttaacaaagc aatttctatg ccaactttta
    20161 aacaaactta taccctttgg aaatactgtc ttacagcatt cattaattct tctaaatttt
    20221 taaacccatc aaaaaaaagt caattattag acactttttt aatcaattta atctataatt
    20281 attgatattg catacatgta taaaaagtgt caaaaaatag acaccgtctt tttatcgaca
    20341 cttttctaac atatactata taatttatca caactttact taaaatatac atttattact
    20401 tattaattct tagttttttg cactccattg gaatcaacta cataattatc tttatacaac
    20461 aaatcagata ttttgactag actgtatcca tcttttttta gtcgacttat tatagtatcc
    20521 aaatagttat ctacatcatt tatattagca tggaataaaa ctattgaacc aggttgagat
    20581 tcttttatta ctttttcaat tacatgattt ggtccaagct ccttccagtc tatagaatct
    20641 acatcccact taattacttt ataacctaaa tcactacaaa tgtccatagc tttattgttt
    20701 acgtccccaa aaggaggtct aaataaatta gtcctttttc ctgttatatt atatattttt
    20761 tctgatgtag tttctatctc ttttgttatg tcttcttttg atatttcctt caaattggca
    20821 tgagtatttg aatgatttcc aatttcatgt cctttttcat ctatagcttt tacaatttct
    20881 tcattatcat ccacccaact accaactaaa aagaaggtag cttttacatt atgtttatct
    20941 aaaattttta aaatatcatc catattatca gttccccatg ctacatcaaa actcaaagct
    21001 atttttttct catttgtatc aactttatat ataggcattt ttgaattgtc agatttagaa
    21061 catatgtatt ttattccata aacaaaaaca aatattaaca ctaataataa tgaaaaaaat
    21121 aaaacactat atatcaaagc tttagtcctt ttaacttctc ttttcatact attccccctt
    21181 tgaaataatt tcttctatat ttaaaattta ttttttttca gctcaaaaaa gtactgtaat
    21241 atttaattta taagacaata actaaaaaaa taaaacctat attgacctaa ttaaactctc
    21301 taaaatactc atttatttaa tttttttata ttttttacat ttttaccttt atttttctgt
    21361 atatattatt tttattttgg agaaattagt attaacatca cataaataaa agtgttgtta
    21421 gacatcataa actaattatt tgaggaggaa atttactatg tcaaattcaa acagaacagt
    21481 agttccagaa gcaaaagctg ctttaaacca aatgaaatta gaaatagcta acgaaatagg
    21541 tttatctaac tatgaaaaca tagacaaagg taacttaaca gctagagaaa atggatatgt
    21601 tggtggatat atgactaaga aattagttga aatggctgaa agacaaatgg ctggaaaata
    21661 aatagtaaat tttgacacct gggtatcaac agggctgcta attagcagcc ctgtttttct
    21721 atatttattt ataaaattgt ttttatatga attattaaag attaataata caatacaaat
    21781 tgttctttta attttgtaca aattagttta atatattata tatagaaata gtttgacgga
    21841 ggatgatatt tatgtttgaa aactcgtctg aagaattagc ctatcacaaa cttttaattt
    21901 tatatatatt agaaaaaatt aaaatggatt taactaattc tcaaataact caagtagtat
    21961 tagagactga gatgatgaat tatttttcat tacaacagct tttatctcag cttatggaat
    22021 ctaagttttt gactacatat aaagactctg acagagaata ttatgctctt actcaaagag
    22081 gcgtagaaag tctagaatac ttctttaatc gaatacctac tagtgttact gaaaaaatag
    22141 acaagtatat agattctaac aaagaaaatc tactagcaga tactcaagta aagtctagtt
    22201 ttgtaaaaca aagtgataat gaatttattg tcaaccttag agtcatagaa aatcagtcta
    22261 acttaataga tttaaatcta aatgtttcat cagagaaaca agcaaaactt atttgcaata
    22321 actggaaaaa taatgcatct tatatgtatg ccgaagttat cgatttactt ataagagaca
    22381 tacattaaag cctacaaaca aattatagtt gtaggctctt ttcctacctt gatttctttt
    22441 attaatttgt tatttgcagt ttcataaact tttactgaat tttcttgtgt atctgataca
    22501 aatagtaact tttcatcttt agatatttct aatccttggg gaatcccatt tatttcaata
    22561 tttccaatgt atctactcgt tgtataatct aaaattctta cattattata acccaaatca
    22621 gcaacataaa cttttttgcc actataatta aatcttattt ctactggtaa taaaaattca
    22681 tataatacct tttttatatc catgcttact tcatctatta ttataagctt tccatcaagt
    22741 gttgatatat acatttcttt cttttgttta tcaagttcta tatgccaagc tctaaagtcc
    22801 atatttattt tcttttgtat atttttatta attgtatcta tacatactat cgagtttata
    22861 cacggtacat ataacttttt gctttctcta tcaaagtcaa atccatgtgg catattatca
    22921 actccaataa ttcctattgg atttaatgtg tttttatcta gtacatatat actattagaa
    22981 tcttcatttg ccacaaacaa ttcattatcg ctaagtacaa cttgactaag acttcctcct
    23041 atagagacag tatcaattat tttatcatta cttaaatcta agacatagag tatcccatta
    23101 cttgaactag gtatgtatat taaatttttt tctttttcta tgcaaaaatg gtgtggatat
    23161 atattatctt ctaatactat ttctttttct agttcaagtg tagaataatc aactatgcta
    23221 atactctttg ataagtagtt agaaatatag attttcatat aacccctcct ctctacaata
    23281 catatactac aataatatat tatttcatta gcttgtatta tgctttatag ttgcattatt
    23341 acgcatatat ttggtattat tattaatgtt aaaaaaagaa ataatattta atggaggtct
    23401 ttttaatgaa agtaacattt aatccaagtg gtgtatgttg tagagaaatg atttttgaag
    23461 ttgatgaaaa taatgtgatt gttgatgctg agtttatagg aggatgtaat ggtaatttat
    23521 caggacttag aagcttaata attggccaaa atgctcttga agttgctgat aaattaaatg
    23581 gtattgactg tggtggaaaa ggtacttctt gcccagacca attatctaaa gctatacgtc
    23641 aatctatata attacttata taaacatcag taaaattatt aaaaacttat tttatataaa
    23701 aagagttgtc cattttttag acaactcttt tttatatttt tatttgtata cttattttat
    23761 aaaattataa tttctttact aaagttctca ctgtaccaat catatataat gaacctgcac
    23821 ttattataat atcatcctcg cttgatgttt ctaatgtata atttactgca tcctctatct
    23881 catgctttga tgttacatca tctacatatt ttaatacttt ttctcttaat atatctgaat
    23941 ttatggctct tgggttactt ggtgttgttg ttattacctt attaaaatgt ggcaacaata
    24001 tctctaaaac actatcaata tctttatctt ctaacatacc tattaataag gttaattttc
    24061 tacctttaaa attcttatca agtgcttttg ccaaagattt tgctccatct tcattatgtg
    24121 caccatctat tataaaaatt ggattttctt ttatcttttc tattcttcct ggccatttag
    24181 tatttataag tccttttctt atagattctt catttatatt tgctaattta tttatgtctt
    24241 ttagatattt tattacacta attgccaaga tagaattgtt tatttgatgt tcgcctatca
    24301 attttatttc aaggtctcta taagtttctt tcattacagt acaatcgtat atttgagaat
    24361 ttatatctga tttttttatg tttatatcat caaaatcaac ttctatatat ttagcttttt
    24421 tctctttaca aactgacttt ataacatcct ttgcttcatc agtctggtca tatactagta
    24481 ctactccatt ttcctttatt attccagcct tttcgtacgc tattttttct attgtatctc
    24541 caagtatacc tgtatggtcc aaacttatag atactatcac acttacaagt gattttgtta
    24601 ttatatttgt agcatcatat ctgccaccta aaccaacttc taatgccaca aagtctacct
    24661 tttgttctga atagtaataa aatgccattg cagtaaccac ttcaaattct gttggatatg
    24721 catagccttc ttttaccata atctctattt tttcttttat taactcaatt attctagcaa
    24781 catcatcctg tggtatgttt tgtccattta ctcttatacg ctcagtaaaa gtttctaaat
    24841 atggagatgt atataaacca actttatatc cacactctct tagtatatta gaaacaaaag
    24901 aacacacaga accttttcca ttagttccag ctacatgtat tatattcaaa gtttcttgtg
    24961 gatttcctaa taattctaat aactttccga tattttctaa tcctaatctt ataccgaatt
    25021 tattagtttg tgatatgtac tctaatgctt cttcatattt cattatctaa ctccccctgt
    25081 attggcattt tctcttatgc tatctaatta tagtatattt tttactttta atctaccgac
    25141 aaagaataca tataatttaa taccttctac attaaattta gctacaaatc tttatttata
    25201 agtttttata tctttccact aattcagtca gtgtatatat tgaaactaag tcatattttg
    25261 agatgtccat ataattaaac tttatgtcaa atctatgttc tagctttttc aacagattaa
    25321 aacaattatc ataaaccaag cctatctcct tattaaaaag gctaccctta aagagttttt
    25381 cattattatc ttcaatatct aagcaatccc taacaacgtc agcaactttc ataagaagag
    25441 catttttttt gtcatacaaa tccaatttaa agtttacaat tctactacac tcatcaaaat
    25501 cacaactatc ccaatgattt ctagttgcta atgattcaag cttatcattt aaatcagata
    25561 ttgttatgcc tggtttttct gataatatct tagctatttt accagtaagc tttgctgata
    25621 aataactatt acattttggt ggtaatctat aggaattatt tatatcacaa cttatatatg
    25681 aattgctatc cataacacat tgaacatcat attttttgtt gtaccaaaat gaatcatctc
    25741 catctaaagt aaatcctctt acacctataa cattgttaaa catagctgga taacttaagt
    25801 caaaatcatt agcgagtgaa catactatta tttttccttt tctaaacaat tcatagcaaa
    25861 cttcaaataa gcccttaaca ctttctactc ctatgactga taaacttaaa ttaattaatc
    25921 ttatgttagt gtctaaaagg tatcttaatg cttcttcaag aacttttata tttgtaatac
    25981 catcttttct caaaattttt attacaaata actctacatc ttcatactct ttctttatta
    26041 ttgatgcaca agcagttcca tgaccatctt catcatcaaa tttatctgat ataaagatat
    26101 aatcatgcat acattcaaaa gcaattcctc caactaaatt atcttttaag taatcatgtt
    26161 ctttgtctat tcctgtatct aatactgcaa ccttaacttt attattcata tcacatatat
    26221 tctccattac aaaagttttt aatatatcta tgttaatagt tcatcttaaa ctttgattta
    26281 agttgcacaa tttatctttt aaatgctata tttcttgcta aaaacataaa ataagtacac
    26341 catatattta atttatattt attatatcaa ttattatcat taaatggtta aatgttaaga
    26401 taaaattttt agatttatat cacaatttag aattatttgt aaaaaaggag attatctttt
    26461 tattagacaa tccccttgta tattttatta atatttatct atgattaagc atcaatttta
    26521 ttttatactt tttgatttta atactataca tctaaatctt atttaggggg aacaattatt
    26581 tatatgatat aaaaatgcta atttgaatga acttgtctta taatcagttt tagaaaatac
    26641 accattctac aaagtatgaa tcaaaaggtg aaaaaatact aaaaataata aatcaacatc
    26701 aattttaatc aatatatgaa aaacttatac caagtatgta taaaaactaa tataaatatt
    26761 attttaacaa ct
//
LOCUS       DBGJFF010000051        26252 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.051, whole genome shotgun sequence.
ACCESSION   DBGJFF010000051 DBGJFF010000000
VERSION     DBGJFF010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26252
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.051"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            61..237
                     /locus_tag="ACOIAF_002058"
     CDS             61..237
                     /locus_tag="ACOIAF_002058"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669068.1"
                     /translation="MNLKKKEKIVKPVLKKLTEIVLNILSKKILNYAIYAISVLIVTK
                     VVINIDLYISILYY"
     gene            338..1075
                     /gene="cas6"
                     /locus_tag="ACOIAF_002059"
     CDS             338..1075
                     /gene="cas6"
                     /locus_tag="ACOIAF_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HGZ2669069.1"
                     /translation="MSRISIFMESCNGKFPNENSMLSVSFIKNILNAENKDFAKNIFN
                     YGEERKNNKQIKDINTAIYIPNLIRSKNELLVDGDIIFNISFYNYSMFSKLYNGILKV
                     KELEYKGYRFKIKNIKIHKEHEIKENGVIFKTMSPIIVKNKEGKYLDVEDSNYIECLN
                     YIANLTLESIRGSGLRKPLEFIPLNFKKRVLKEKIRGFKVREYYYINAYAGTFFLKGD
                     MEDLNALYKMGIGYRRTENAGMVDILK"
     gene            1080..2555
                     /locus_tag="ACOIAF_002060"
     CDS             1080..2555
                     /locus_tag="ACOIAF_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669070.1"
                     /translation="MREIKAKFYRSGDIVRDIGIVYFYELLMDLKNELEKNDYKLKFT
                     CELNRNYLSLESAEYIDPKYISDYILENQLFKVFKNGKKETLEKIFSNIDNLTYVNFI
                     EELDKSSATEKQKEGIKKDFKNRRFPYVRNSGKYGLNTSLENMETNVKRIVDTVIKSN
                     IEIDEIDKLDLTQYEEKDSVCNVCNINKTTKLDIDLRERKDSKYNFLFRGAEKSGFKR
                     SGQVESNICFECEFFNLMCLLYINLKRPMVFAYTNDLRELAFLNHKIMLKRKMYSDKS
                     FYKKLLHEKISSIRLYRFDIDTNKGIILKFDSIIEYKELLKKIELIDIIDNYNFSREP
                     VNTKNLGKKMIDNGNLSNLKELLLDNISFLKQSTGTSQEMDFVSSSYNIGLYIKLCWI
                     VDGGEEYKMKNYMESNLIYSRVGKDLFNKMTDESRRKNFSMKVIQLLKSNDRTQLFQT
                     IMHVLVSNGILIGEGFVEGLMQSSELELNTNVGLFIQEIMK"
     gene            2575..3588
                     /locus_tag="ACOIAF_002061"
     CDS             2575..3588
                     /locus_tag="ACOIAF_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein
                     Cas7/Cst2/DevR"
                     /protein_id="HGZ2669071.1"
                     /translation="MNEKRVKGVTLTILTESPVPLSYDQGYGNYTPIKKEQYREKIHA
                     KTSIATITYDLRRMLHQEYGWNLSNIIFGKKGNIYPSIKKNVGCVDENGLETDVFGYF
                     IPLKDKGSISKASPLRIIPFRSLNPYRGSTQLITNRGFLSSEFGRKYYDEKEESEVPR
                     DENFPTTQALAIEETLSDYYVYTITLELDRIGVVEVEDGKLLLPEERKFMSKELREKA
                     VKDILDAITVFTRNIKHSSVLLKPLAVMGGAFDKVVPFFWDDVDYNADSGEINLEGVI
                     ETIESYSLKESNTILAINDRLKISNKKELNKFNLSKYPVKEIKNLADRLEIGEDNMWY
                     LKE"
     gene            3604..4401
                     /gene="cas5"
                     /locus_tag="ACOIAF_002062"
     CDS             3604..4401
                     /gene="cas5"
                     /locus_tag="ACOIAF_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas5"
                     /protein_id="HGZ2669072.1"
                     /translation="MKVLQCKLKQPTAHYRDPKVFQNEYISTLNLPSKTTIMGMITYL
                     CDRRLKSDIDIGIIGTHHHRELEFSRGENIDFWNEYTNMRKGKDKEKFLLKGNYYDYY
                     KEHKAQNSILNYEVLKEVELTIFISCKDDEELEFIRKKLESPCKYANLGRKEDFVIPS
                     EKGCFVKEVALKEVIPMNTRDAIKENIKLKNTYVRVDLRDKENVETIINQGVLIALPH
                     KYKDLEANRDDRVYEFCHYIYVDNDGIYPKNIKVNVCIDTKEVFTWL"
     gene            4428..6836
                     /locus_tag="ACOIAF_002063"
     CDS             4428..6836
                     /locus_tag="ACOIAF_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HGZ2669073.1"
                     /translation="MKNIMQKEILAKSYNLRTNQQEQTLVEHIKDLFEVLESILELNL
                     YSDKDVEILKICCALHDLGKINSIMQQKMEINNKISYSCSEEERKKLESDKKSLKKIA
                     RHNIFSGAFLKDILEKMNLSEEDKLYIYKSIMLHHGNYEDYMRLSTSKVQKEIYDYIE
                     KGILEKEDFNLEDIEDYIKEILDVDFKFNEDVLDYDYIDKLSESMFIDSDYNNGQIDD
                     SVLNYRKFKYILYKGMLNLIDHSASSRQKGIKFYNDFTDEEIDNMILNEIYKSQGNLE
                     KNNIEFNTIQKRLRELSGRNVLTVAFTGSGKTAADYRKTFKRKFFLVPNKISAESFYR
                     KNIFQNKNDLDTRSSNDYIGLLHGDINLYSENEDNGEHDFVLTLRDIDLSINFCKPCV
                     IATVDQLLLSMFKFPGYEKIFAAVKNASITVDEVHLLEPKMFLILIYFMQFACKYLDV
                     DFHLMTATLPKSYKEQMINKGIIFQEESNENVTDKGEIVFIESNKEEEICEGKDVKVS
                     FIKEKEIKSIVEDALENKQKILIIKNTIDSVNRTYEFLKENLSDKYGGVDIDVLHSRF
                     KFKDKKEKYSKILNGEGDIWISTQSVEISLDLDFNIIISDLATMDSLIQRMGRCNRNN
                     KYEYGNFYILPSEDKIYDNKLKNTTKSILKDILKTNSIFTMGIRKTILDDYYDNSVVK
                     KYFEENFISCDKEIKNIYGINKEIFDGLDLIFNFEPYKNIVDSKKEAAKIFRDVDVSY
                     KIILEEDFYKEDRELQQDSIQVSGFIFNRLYYYGLISKVEGYMVLKSSSKFEYNSTVG
                     LILK"
     gene            7277..8224
                     /gene="prmA"
                     /locus_tag="ACOIAF_002064"
     CDS             7277..8224
                     /gene="prmA"
                     /locus_tag="ACOIAF_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HGZ2669074.1"
                     /translation="MNNWIEITIKTTTEAVEPITNILYEQGAGGAVIEDPKDFLFQKK
                     NELDWDYVEEEVFKKNEEDDVLIKTYVSEEKNVMEFVEIIKQKVLGLKDFGIDIGEGS
                     VSLDQVNEADWANAWKAYYKPTKVGQRVVVKPTWEDYAMQDGDLIIELDPGMAFGTGT
                     HETTSMCIRELEKYVNKDSKVFDIGCGSGILAIAAAKLGAKEVVAVDLDEVAVKVAKE
                     NVLENKVEKSVSVMHGNLTDVIKDKADVIVANIIADIIKILAKDVQNFMKEDAIFISS
                     GIILDKVEEVKESLIENGFEIVEVQKLGEWSAIVSKLKK"
     gene            8243..9001
                     /locus_tag="ACOIAF_002065"
     CDS             8243..9001
                     /locus_tag="ACOIAF_002065"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HGZ2669075.1"
                     /translation="MDRFFVEKNNINLQDKTCTIEGEDVKHISKVLRCKLGEKLEICD
                     KNNNEYICEIMNIDKSIVNLEILEKVDINRESELKVRLYQGLPKAPKMEMILQKLTEV
                     GVEEIILVQTKRSVVKVDDKKEDKKFERWERIIYEAAKQSKRGKIPKLRGVLSFKEAL
                     EDMKKNNVNICPYENERTVSIKHALKKCDSNIDSVGIFIGPEGGFSEEEIEQIQKNNC
                     NVVSLGPRILRTETASVVASTIALYELSDLGGEK"
     gene            9003..10301
                     /gene="mtaB"
                     /locus_tag="ACOIAF_002066"
     CDS             9003..10301
                     /gene="mtaB"
                     /locus_tag="ACOIAF_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0061712 - tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HGZ2669076.1"
                     /translation="MKKVAFYTLGCKVNQYETEAMLELFEKDGYEQVNSEEYADVYVI
                     NTCTVTHMSDRKSRQYIRRVKKKNPDAIIAVVGCYSQVSPEEILDIEEVNLVMGTNDR
                     RKIVEEIKKINSSKKVSTVDDIMKVKAFEEIEISQTNGKTRAFMKIQDGCDRFCTYCI
                     IPYARGRVRSRDIDSIVDEVKKLANNGYKEVVLTGIHVASYGKDLKDRDIKLLDVIKQ
                     INQIEKIERIRLSSVEPILFTDEFVNEVLKMDKVCPHYHLSLQSGCDETLKRMNRRYT
                     TLEYKTIVDRLRSKMPDVAITTDVIVGFPGETNEEFKKTYEFLKEIELSQMHIFKYSP
                     RKGTPAATMENQVDPQMKHFRSEQLLNLSKVNFNKFATKFIGRELDVLFEQNIEGNKY
                     EGLTSNYIRVVVESDKNIQGQILKVKINDVKDEYVEGILL"
     gene            10344..10694
                     /locus_tag="ACOIAF_002067"
     CDS             10344..10694
                     /locus_tag="ACOIAF_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HGZ2669077.1"
                     /translation="MDCIFCKIANGEIPSTKVYEDDRVLAFNDLNPVAPYHILVVPKK
                     HYDSLIDIPDKEMDIVSHIHVVINKIAKEKGFDQTGFRVINNCGSDGGQEVKHLHYHI
                     LAGKKLPNYEAGQN"
     gene            10857..11036
                     /gene="rpsU"
                     /locus_tag="ACOIAF_002068"
     CDS             10857..11036
                     /gene="rpsU"
                     /locus_tag="ACOIAF_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HGZ2669078.1"
                     /translation="MSEVRVRENETLDSALRRFKRQCAMSGIMSEVRKREHYDKPSVK
                     RKKKAEAARRKNAKK"
     gene            11066..11509
                     /locus_tag="ACOIAF_002069"
     CDS             11066..11509
                     /locus_tag="ACOIAF_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HGZ2669079.1"
                     /translation="MSLKQKLQEDLKSSMKNKDTVRKSVVTLIRASIKQYEVDNRVEL
                     DEDGIIDVIAKQLKQRRDALVEFEKAGREDLIKETEGEIEVLKEYLPQQLSEEELEEI
                     VKSTISEVGATSMKDMGKIMSVIQPKVKGRADGKLINKLVKQNLQ"
     gene            11620..12117
                     /locus_tag="ACOIAF_002070"
     CDS             11620..12117
                     /locus_tag="ACOIAF_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HGZ2669080.1"
                     /translation="MIISVKEIRNFLINIFIPLIIGYLSSILTMIISGTDISTYYLQL
                     TKPSFAPPAFIFPIVWTILYILMGISSYLILRKGYNLPKVRDAIFYYGLQLVLNFIWS
                     ILFFGFGLRFTALIDIIIMILVSIIMISKFSKIDKRSGYINLIYLMWLVYAGFLNYFI
                     WFINK"
     gene            12310..12873
                     /gene="raiA"
                     /locus_tag="ACOIAF_002071"
     CDS             12310..12873
                     /gene="raiA"
                     /locus_tag="ACOIAF_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HGZ2669081.1"
                     /translation="MQIIVSGRQMKLTDGIKGYVDGKLSRLEKYLDPESEVKVTVSAK
                     KDRQKVEVTIIPINGQIIRAEDVEDDLYAAIDIVCDKLSRQVVKYKTKVKDKVQNNKS
                     IRFENLDFIDNSSEFDDYDYDEEEDENIVIERRKKFNVKPMSSEEAILQMELVGHNFY
                     MFRNQDNFEINIVYKRKAGGYGLIEQD"
     gene            13086..13322
                     /locus_tag="ACOIAF_002072"
     CDS             13086..13322
                     /locus_tag="ACOIAF_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YabP/YqfC family sporulation protein"
                     /protein_id="HGZ2669082.1"
                     /translation="MLEISTDLQVNQPVITVTSNTFISIENYLSILEYEVDLIRIKTK
                     VKTIKISGDKLSLKYITDSEIGIKGIIYNVEYVD"
     gene            13335..14678
                     /gene="yqfD"
                     /locus_tag="ACOIAF_002073"
     CDS             13335..14678
                     /gene="yqfD"
                     /locus_tag="ACOIAF_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfD"
                     /protein_id="HGZ2669083.1"
                     /translation="MISFIRGYYVIVVEGVGLEQFLNHLIRNGISVYNVTRIKNTKME
                     FHIDRQDIKEFKNVYRGSKFDIKVKQKTGVPFIIKRVYKHKGMWICALVSLFLLMSTS
                     QFVTDVYIQSPEGIKKEALRNELYKVGVRPGVYKKSIDRKEVRDHMMSKFNDVAYLSI
                     NVKGTNIFVTVTKKAESLKSTDQSNYCNVIALKNGIIEKVIPRSGKSVIKSGDIVQKG
                     DVLLNGANTKSIPEVWASTFYESTKKASYVDTVNKKTGEKKNIYTLSFYDKEFTMRKN
                     IKYKDYVVENKEKKLSIGNYTFPIKIKTSTFYETKKVEVKRNKEELKKELSEKALKEL
                     EYIIPASARIIDVKHNFKVNKNMLEYLITVQTSENIAKIYPLSKSEAERFIKEESKPD
                     EGEEEVPSNPEKRPLNDIRNEFDEDNKDNKDQNNSDENNSNQNSNNSQNNNSNNN"
     gene            14617..15732
                     /locus_tag="ACOIAF_002074"
     CDS             14617..15732
                     /locus_tag="ACOIAF_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HGZ2669084.1"
                     /translation="MKIIVIKIVIIVKIIIAITTNKIKLKILGGLIQLIIQKKFTIAD
                     GNFERELFGNFDENVKLIEKTLNIDVILREGNIILIGEEKNVDSALKLMNELHQTVSN
                     GKHLDKQSISYSLSLLLEGSEQKIKELEGTIVITQRGKAVQPKTLGQKEYIKLIENND
                     ITFGVGPAGTGKTYLAVAMAVKAFKRDEVSRIILTRPAVEAGESLGFLPGDLKDKVDP
                     YLRPLYDALFEMLGADKFNKYLERGTIEVAPLAFMRGRTLDNSFIILDEAQNTTSEQM
                     KMFLTRLGFGSKAVVTGDVTQTDLPQNKKSGLIQATEILKGVPGIGSIMLTDRDVVRH
                     ELVQRIIRAYEKHDKREEFKKEERKKAKMQEKTFKRK"
     gene            15789..16250
                     /gene="ybeY"
                     /locus_tag="ACOIAF_002075"
     CDS             15789..16250
                     /gene="ybeY"
                     /locus_tag="ACOIAF_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HGZ2669085.1"
                     /translation="MDLILDDRQDKLEVSEELIEKIKDIIIECLDYEGYDDNYEVSLS
                     FVDNKEIHELNREYRGVDRVTDVLSFPLLSDDFEDVELEEESLGDIVVSLERALEQSI
                     EYNHSFEREVCFLICHSMFHLLGYDHDTDENTKEMREKEEHILNKLNITRE"
     gene            16256..16975
                     /locus_tag="ACOIAF_002076"
     CDS             16256..16975
                     /locus_tag="ACOIAF_002076"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HGZ2669086.1"
                     /translation="MKPEKTRQGIIKAFNAAIEGILYTFKFERNMKIHYLGSVAVLII
                     SLFFNFSKLEMIMLLMSICLVVVAEMFNTAIEKAVDLVTDEYHVLAKIAKDVAAGGVL
                     VAALNSVVVGYILFYDKLTDISGILIYKIRESELHITLICILLVLIAVVVVKALTSTG
                     TPLKGGMPSGHAALAFAIATAITLMTERVVASTLAYIMAVLVAQSRIEGKIHTFWETI
                     AGALLGVLIAILVFQLGMFYN"
     gene            17094..17495
                     /locus_tag="ACOIAF_002077"
     CDS             17094..17495
                     /locus_tag="ACOIAF_002077"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HGZ2669087.1"
                     /translation="MDNIELLRLAEDARQHSYAPYSGFRVGAALLTKSGKVYTGCNIE
                     CASLGGTNCAERTAIFKAISEGDKDIYKIAIASDNSENNEQTYPCGICRQVIIEFGSD
                     IKIITGYTKGEVFEHSIKDLLPNYFSGNDLK"
     gene            17559..18452
                     /gene="era"
                     /locus_tag="ACOIAF_002078"
     CDS             17559..18452
                     /gene="era"
                     /locus_tag="ACOIAF_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HGZ2669088.1"
                     /translation="MFKSGFVSIVGRPNVGKSTLMNNVVGEKIAIMSDKPQTTRNTIQ
                     AVYTDEEMQIVFLDTPGIHKPKNKLGEFMVKAATEAFKNVDLILFVVDDSKKIGPGDR
                     KIIEDLRSVKTPIILVVNKIDQLDQKDELFDIIKMYDREGIFKEIVPISALKGKNTDT
                     LIKVIQNYLEEGPKYFPDYMITDQPERVLIAELIREKVLHYLNDEIPHGVAVEIEKMK
                     ARNDKEIVDVSAVIYCERDSHKGIIIGKNGRKLKGIGKSARQDIELLLGSQINLQLWV
                     KVKENWRNLQNYINNFGYNDK"
     gene            18464..19843
                     /gene="mgtE"
                     /locus_tag="ACOIAF_002079"
     CDS             18464..19843
                     /gene="mgtE"
                     /locus_tag="ACOIAF_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HGZ2669089.1"
                     /translation="MDRKLDASQDLLYEVKSLIDNNKVLELRELIEEYHIIDIFDIME
                     NLEEDMKIQLFEVLPLDMASSILEEGSVEFFISILSKLDVEHSKNILELMSLGDMADK
                     LSELEEEEREHIINLLNQENADYVKELLFYDEDSAGGTMTTGYISINKDMTALEAIDH
                     MREEAEEAETIYYIYVVDDEEKLVGVLSLRELIIARDANIVEDLMSENIISVYVDEDR
                     EEAVRLVSKYNLIAIPVVDRQEKLKGIITVDDIIDVMEEEATEDMYKFAGSSEHEREV
                     AEKENPTLREQIISALRGRLPWLIITLVGGLLASLILSNLDYIMNPVYAPLVFFIPVV
                     IGMGGNIGTQSSSVTVITLSNKDLNFSNVVREGIVGIITGLLCSIITGIVIYFVMRKL
                     DIVLIVSISLFINMVLGATIGAFMPVLLKKMDADPSTVSSPIISTALDITGIAVYFII
                     TTALLSKIV"
     gene            19916..20071
                     /locus_tag="ACOIAF_002080"
     CDS             19916..20071
                     /locus_tag="ACOIAF_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqzL family protein"
                     /protein_id="HGZ2669090.1"
                     /translation="MNNISWEVFKKTGSIDAYLYFCDCKNIHEEIEEVREKKEDDDNP
                     EHPGDST"
     gene            20037..20810
                     /gene="recO"
                     /locus_tag="ACOIAF_002081"
     CDS             20037..20810
                     /gene="recO"
                     /locus_tag="ACOIAF_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HGZ2669091.1"
                     /translation="MIILNTQGIVLKAIRYKESDIILTLFTRKLGKVSAIAKGAKKNK
                     SSLLSSSQLFSYSNFTLKKQGNMYKVTQSEIIKSFYNISYDIEAFSYATYITKLVENS
                     ILENQTNNRLFILLAQTLYLYTQDNTDNRFITAAFELKFLDYIGFKPIVNKCINCECK
                     ILQNSVFNIYEGGILCSKCSKLFDNNIKLDLTTISLMEYVLRNDILTCSKAKVSKYIT
                     HELENILDKYLKVYVDNINFKSLHVLQSVKNNKGVDNDE"
     gene            20803..21402
                     /locus_tag="ACOIAF_002082"
     CDS             20803..21402
                     /locus_tag="ACOIAF_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HGZ2669092.1"
                     /translation="MSSNITIEMVDSVFERVPGATYAEAKEALVMCNGDVIEAVIYLE
                     NKKNTCNCKAKSAKETMEEVFGKDGENIKFQLKELLRKSSVVRIIVEKDGKTMMNIPL
                     TVGVVGLAFVPLATLVGLSAAVISKYRIKVQNEEDGEIVDLGELNEEKLHVLKNMITN
                     AAKDVKDVVVDKKEDDKDVTADLMKEESEKNKEDEDENK"
     gene            21562..22440
                     /gene="glyQ"
                     /locus_tag="ACOIAF_002083"
     CDS             21562..22440
                     /gene="glyQ"
                     /locus_tag="ACOIAF_002083"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HGZ2669093.1"
                     /translation="MNFQEMILALQKYWSKQGCIMMQPYDIEKGAGTMNPNTFLRSLG
                     PEPWQVCYVEPSRRPADGRYGENPNRLYQHHQFQVILKPSPDNIQELYLESLKEIGID
                     PSEHDIRFVEDNWEAATVGAWGLGWEVWLDGMEITQFTYFQQVGNIECELETGEITYG
                     LERLAMYIQEVDSVYDLKWNDKITYGEVFNKAEYENSMYAFELCDADMLFNLFDIYEK
                     EALRLMENGLVIPSYDYVLKCSHAFNTLDARGAIGVSQRASFIGRVRNMAKTVAETFV
                     KQREEMGFPLLKDGDK"
     gene            22440..24506
                     /gene="glyS"
                     /locus_tag="ACOIAF_002084"
     CDS             22440..24506
                     /gene="glyS"
                     /locus_tag="ACOIAF_002084"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HGZ2669094.1"
                     /translation="MKNYLLFEIGVEEMPSRFVGSTLEQLKNNLSKLFDENRIAFDKI
                     NAYGTPRRLTLVVEGLSDRQNDLEEEIKGPAKKIAVDAENNLTKPALGFIKSKGLSES
                     DIYFKQVGKEEYIFATIKQDGKETSEVLKDILPETIKSVVFPKAMRWGGKNLKFARPI
                     RWIVALLNDKVLEFDLEGIISSNVTKGHRFLGESTIEVDSIEDYFEKLEKNYIVLDQN
                     KRKEMIRKQCIEVANSLGGEVEFDEDLLEEVTYLVEYPTAFYGEFDVDYIKLPKEVVI
                     TPMKQHQRYFPVLKEGKLLPNFIAVRNGDSYRIDNVKAGNEKVLEARLADALFFYRED
                     TKRNLESYIEKLKTVVFQVKLGTIYDKTLRLEKLSADILDSLGLVDEKADTIRAAKLS
                     KADLVTGMVGEFDELQGFMGKEYAKVGGENDVVSEAIFEHYLPRFAGDILPKTNAGVA
                     LSIADKLDSIAGFFAIGIQPTGSQDPYALRRQALGILSILMDRKVAVDIKVLIEHALD
                     NYSELDFDKEEVVEQIMNFFNERVKNLFKDLGIRYDVVDAVLSSDINDVSDMHVRALE
                     LNNWLEKDELVEMLTAFNRVSTLAQKAESDKIDESLLKEDAEIKLYNEFKQVKVKVNE
                     LLKDKKYGIALDEFASLRPVVDNLFDTVMVMDNDEAIKNNRLALLKQVYDTMLEICDL
                     SKIVYK"
     gene            complement(24672..26219)
                     /locus_tag="ACOIAF_002085"
     CDS             complement(24672..26219)
                     /locus_tag="ACOIAF_002085"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HGZ2669095.1"
                     /translation="MDNFKKILLNELPNFKEYSSKFLNGEINKMQYKGFSGGYGVYAQ
                     RDKKSFMIRLRVSAGVLSQYQLNKIYEIAIKHNLDKIHLTTRQAIQLHDLSINSIVDI
                     MEEGIKNDIFTRGGGGNYPRNVGLSPLSGVDPSEVFDVTPYAVATDKYFIKNATTYHL
                     PRKLKVSYSNCYTDTAHCSIQDLGFVATLKNDEPYFRVFLGGGLGKQPKVALELDELI
                     KPKDALYCVEGMIKFFMDYGNYENKNRARVRYMVESLGEELFLEKFKEYYKLEKENGS
                     LELNIEEIDYSKPGVKIDIQDSRLIPQKQDGLYTVYIHPVGGILLTKDLSTLLKELDN
                     VENPMIRLGMTEGLYILNLNGNEAKRILEISKTISCNSQLEQSISCIGVPICQMGIQN
                     SQKMLHEIIDYFRLQNNEEILNKAPKLYISGCLNSCGVHQVGSIGLCGKKKNVDGIST
                     DAFELFVGGSFEIGKTRLGKSLGDFKASDIPEMLYKISDASSGNFYEWVNSNDNILNK
                     ITDKYKI"
BASE COUNT        10756 a         2578 c         4714 g         8204 t
ORIGIN      
        1 tactgtaata taatggaaat acagtttaat aaatataaaa atagaaagag ggaattttaa
       61 atgaatttga agaaaaaaga gaaaatagtt aagcctgttt taaagaagct tacagaaata
      121 gtattaaata tattatcaaa aaaaatatta aactatgcta tatatgcaat ttctgttttg
      181 atagtaacaa aagttgttat aaatatagat ttgtacatat ccatattgta ttattagata
      241 tcttatagat ataaaaaatt aatagatata tatctattaa ttttttatat tacaaataga
      301 gaataatgaa ttatactaga aagtaggagg tgaaaatatg agcagaattt caatttttat
      361 ggaatcatgt aatggcaaat ttccaaatga aaattcaatg ttgtcagttt cctttataaa
      421 aaatatttta aatgcagaaa acaaagattt tgctaaaaac atatttaact atggggaaga
      481 aagaaaaaac aataagcaaa taaaggatat aaatacagcc atttacatac caaatcttat
      541 aagaagcaaa aatgagttac ttgttgatgg agatataata ttcaatataa gtttttataa
      601 ttattctatg ttcagtaaat tatacaatgg aattttaaaa gtaaaggagc tagaatataa
      661 aggttataga tttaaaataa aaaatataaa aatacataaa gaacacgaaa taaaagaaaa
      721 tggtgttata tttaaaacta tgtctcctat aatagtaaag aataaagaag gtaaatattt
      781 agatgtagaa gattcaaatt atatagaatg tttaaactat atagctaatc taactctgga
      841 gagtataaga ggtagtgggc ttagaaagcc attggaattt atacctctta acttcaaaaa
      901 aagagtgtta aaagaaaaga ttcgtggatt taaagtgaga gaatattact atataaatgc
      961 ttatgcagga acattttttt taaaaggaga tatggaagat ttaaatgcac tgtataaaat
     1021 gggaattgga tatagaagga cggaaaatgc aggaatggtt gatatattaa agtagcgagg
     1081 tgagagaaat aaaagctaaa ttttatagat caggtgatat tgtaagagat attggcattg
     1141 tttactttta tgaattacta atggatttaa aaaacgaact agaaaaaaat gattataagc
     1201 taaaatttac ttgtgagtta aatagaaatt atttatcttt agaatctgca gaatatatag
     1261 accctaagta tatatctgat tatatattag aaaatcaact ttttaaggta tttaaaaatg
     1321 ggaaaaagga aaccttagaa aaaatattta gtaatataga taatttaacg tatgtaaatt
     1381 ttattgaaga gttagataaa tcttctgcaa ctgagaaaca aaaggaaggg attaaaaaag
     1441 attttaaaaa cagaagattt ccatatgtta gaaatagtgg taagtatgga ttaaacacga
     1501 gtttagaaaa tatggaaaca aatgtaaaaa gaatagtaga tactgtaatt aaatcaaata
     1561 tagagataga tgaaatagat aaacttgatt tgacacaata tgaagaaaaa gatagtgtat
     1621 gcaatgtttg caatataaat aaaacaacaa aactagatat tgatttaaga gaacgaaagg
     1681 actctaagta taacttttta tttagaggtg cagaaaaatc aggctttaaa agaagtggac
     1741 aagttgaaag taatatttgt tttgagtgtg aatttttcaa cttaatgtgt cttttatata
     1801 taaatttaaa aagaccaatg gtttttgcat atacgaatga tttaagagaa ttagcatttt
     1861 taaatcataa gataatgctt aaaagaaaaa tgtattcaga taaatcattt tataaaaaac
     1921 ttttgcatga aaaaataagt tcaattagac tatatagatt tgatattgat acgaataaag
     1981 gtataattct aaagtttgac tctattatag agtataaaga attattaaaa aaaattgaac
     2041 ttatagatat tatagataat tacaatttct caagggagcc agtaaacact aaaaatttag
     2101 gtaaaaaaat gattgataat gggaatttat caaatttaaa agaattatta cttgataata
     2161 taagtttttt aaagcaatca actggaactt ctcaagaaat ggattttgta tctagctcat
     2221 ataatatcgg tttatatata aaattgtgtt ggattgtaga tggaggggag gagtataaaa
     2281 tgaaaaacta tatggaaagt aatttaatat attcaagagt aggtaaagac ttatttaata
     2341 aaatgacaga tgaatccaga agaaaaaatt tttctatgaa agtgattcaa ctacttaagt
     2401 ctaatgatag aactcagcta tttcaaacta taatgcatgt gttagtttct aatggaatct
     2461 taataggaga agggtttgta gaaggtctaa tgcagagttc tgaattagag ttaaatacaa
     2521 atgtaggtct atttattcaa gaaatcatga aataaaaaag ggggaaaatt taaaatgaat
     2581 gaaaaaagag taaaaggagt aacattaact atactgacag aaagcccagt tccactttca
     2641 tatgaccaag ggtatggaaa ttatacacca ataaaaaaag aacaatatag agaaaaaata
     2701 catgcaaaaa ctagtatagc cacaataaca tatgatttaa gaagaatgct ccatcaagaa
     2761 tatggttgga atttaagtaa tattattttt ggaaaaaaag gaaatatata tccatctatt
     2821 aagaaaaatg taggatgtgt agatgaaaat ggattagaaa ctgatgtatt tggttatttt
     2881 ataccactta aagataaagg ttctataagc aaagcttctc cacttagaat aatacctttt
     2941 aggtcattaa atccatatag aggttcaaca cagttgataa caaatagggg ttttttaagt
     3001 agtgaatttg gtagaaaata ttatgatgag aaggaagaaa gtgaagtacc tagagatgaa
     3061 aactttccaa caacacaagc attggcaata gaagagactt tatcagatta ctacgtttat
     3121 acaataacat tggagcttga tagaatagga gtagtagaag tagaagatgg aaagttactt
     3181 ttaccagaag aaagaaaatt tatgagtaag gaattaagag aaaaagcagt aaaagatata
     3241 ttagatgcaa ttactgtgtt tacaagaaac ataaaacatt catcagtact attaaaacca
     3301 cttgcagtaa tgggtggagc ttttgataaa gttgtaccgt ttttttggga tgatgtagat
     3361 tataatgctg atagtggtga gataaattta gagggagtta tagaaactat agaaagttat
     3421 agccttaaag aaagtaatac aatacttgct atcaatgaca gattaaaaat ttctaataaa
     3481 aaggaattaa ataagtttaa tttaagtaaa taccctgtaa aagagattaa aaatctagca
     3541 gatagattgg aaattggaga agataatatg tggtatttaa aagagtagga ggttttttga
     3601 attatgaaag tattacaatg taaacttaag caaccaactg ctcactatag agacccaaaa
     3661 gtatttcaaa atgaatatat aagtacttta aatttaccat ctaagactac aataatgggg
     3721 atgataacgt atttatgtga tagaagatta aaatctgata tagatatagg aataattgga
     3781 acacatcatc atagagaact agaattttca agaggagaaa acattgattt ttggaatgaa
     3841 tatacaaata tgagaaaagg taaagataaa gaaaagtttt tgcttaaagg aaactactat
     3901 gattactata aagagcataa agctcaaaat agcatattaa actatgaagt tcttaaagaa
     3961 gtagaactga ctatatttat ttcttgcaag gatgatgaag aacttgaatt tattagaaaa
     4021 aagcttgaaa gtccatgtaa atatgcaaat ttaggaagaa aagaagattt tgtaatacca
     4081 agtgaaaaag ggtgttttgt aaaagaagtt gcgttaaaag aggttatacc tatgaatact
     4141 cgtgatgcta taaaagaaaa tataaaatta aagaatacat atgtaagagt tgacttaaga
     4201 gataaagaga atgtcgagac tataataaat caaggtgtat tgattgcatt acctcataaa
     4261 tataaagatt tagaagcaaa tagagatgat agagtttatg agttttgtca ttatatatat
     4321 gttgataatg atggtatata tcctaaaaat attaaggtaa atgtatgtat tgatacaaaa
     4381 gaagttttta catggcttta aattattttg aaagataggt gtttatcatg aaaaatatca
     4441 tgcaaaaaga aatattagct aagtcataca atttaagaac taatcaacaa gaacaaacat
     4501 tagttgaaca tattaaggat ttatttgaag tattagaatc tatattagaa ctaaatctat
     4561 attcagataa agatgtagag atattaaaaa tatgttgtgc tttgcacgat ttaggaaaaa
     4621 ttaattcaat tatgcaacaa aaaatggaga ttaataataa aatatcgtat agttgctcag
     4681 aagaagagag gaaaaagtta gagtctgaca aaaaatcttt aaaaaagata gcaagacata
     4741 atatattttc tggagcattt ttaaaagata tattagaaaa aatgaacttg tcagaagaag
     4801 ataagttata tatatataaa agtataatgt tacatcatgg aaactatgaa gattatatga
     4861 ggttaagtac tagtaaagtt caaaaagaaa tttatgatta tattgaaaaa ggtattttag
     4921 aaaaagaaga ttttaatttg gaagatatag aagattatat aaaggagatt ttagatgtag
     4981 attttaaatt taatgaagat gtattggatt atgattacat agataaatta agtgaaagta
     5041 tgtttataga cagtgattat aacaatggtc aaatagatga ttcagtttta aattatagaa
     5101 agtttaagta tattttatat aaagggatgc tgaacttaat agaccatagt gcatcaagta
     5161 gacagaaagg tataaaattt tataacgatt ttactgatga agaaattgat aatatgattt
     5221 taaatgagat atataagagt cagggtaatt tagaaaaaaa taatatagag ttcaatacta
     5281 tacaaaaaag gttgagagaa ctttctggaa gaaatgttct gactgttgca tttacaggtt
     5341 caggaaaaac agctgcagat tatagaaaga cttttaaaag aaaattcttt ttagtgccaa
     5401 ataaaatatc agcagaaagt ttttatagaa aaaatatttt tcaaaataaa aatgatttag
     5461 atacaagaag tagtaatgat tacattggat tacttcatgg agatataaac ttatattcag
     5521 agaatgaaga taatggagaa catgattttg tattgacatt aagggatata gatttgagta
     5581 taaacttttg caaaccttgt gtaattgcaa cagttgatca gttattactt agtatgttta
     5641 aatttccggg atatgaaaaa atattcgcag ccgtaaaaaa tgcatctata actgttgatg
     5701 aagtacattt attggaacca aagatgtttt tgattttgat atattttatg cagtttgcat
     5761 gcaagtactt agatgttgat tttcacttaa tgacagctac acttccaaaa tcatataaag
     5821 agcaaatgat aaataaggga attatttttc aagaagaaag taatgaaaat gttactgata
     5881 aaggagaaat agtttttatt gagagcaaca aggaagaaga gatttgtgaa ggtaaagatg
     5941 taaaggtttc ttttataaag gaaaaagaaa tcaagtcaat agttgaagat gctttagaaa
     6001 ataaacaaaa aattcttata ataaagaata ctattgattc agtcaataga acatatgagt
     6061 ttttaaaaga aaatttatct gataagtatg gtggtgtaga tatagatgtt ttacatagta
     6121 gatttaagtt caaagataaa aaagagaaat atagtaaaat attaaatgga gagggtgata
     6181 tttggatatc aactcaatca gtagaaatat ccttggattt ggatttcaat ataataataa
     6241 gtgatttagc aactatggat agcttaatac aaagaatggg tagatgcaat agaaataata
     6301 aatatgaata tggaaacttt tatattctac ctagtgaaga taaaatttat gataacaaac
     6361 ttaagaacac aactaaaagc atcttaaagg acatattaaa aactaactct atttttacaa
     6421 tggggattag aaaaactatt ttggatgatt actatgataa tagtgtagtt aaaaaatatt
     6481 ttgaagagaa ttttataagt tgtgataaag aaattaagaa tatttatggt ataaataaag
     6541 aaatttttga tggattggat ttaattttta attttgaacc ttacaaaaat attgttgata
     6601 gtaagaagga agcagctaaa atttttagag atgttgatgt tagttacaaa attattctgg
     6661 aagaagattt ttataaggaa gatagggagt tacaacaaga ttcgattcaa gtatcagggt
     6721 ttatatttaa tagattatat tattatggat taataagcaa agtagaagga tacatggtac
     6781 taaaatctag tagtaagttt gagtataata gtactgtagg cttaattttg aagtgagcat
     6841 atgtttaaat ataatatacc tttagacatt gaaaatacaa aaagtactac tgtaagtcca
     6901 ctgtaaaaac tattttttaa aaggaaaaat aagttgtgag atagagtgtt tacaagtgta
     6961 tatagataat aaatttgagg tttaagatta actaagtggg tttcaggtac atcgcgaaaa
     7021 taattataag ttggagattt tgtatgttta agattaacta agtggatttc aaatattgat
     7081 atagtagata taatagaagt tttattatta ggtttaaaat taactaatcg gatttttaaa
     7141 tattctgact attatataag gatattatta gcttgaattt aaaaccaata atataaaaca
     7201 acaaaatatt atatttatga tataataaaa gttatggaaa aacaggaata ttactataga
     7261 gaagagaggt aaacttatga ataattggat agaaataact ataaaaacaa caacagaagc
     7321 agttgaacct ataacgaata tactatatga acaaggagct ggaggagctg taatagaaga
     7381 cccaaaagac tttttattcc aaaagaaaaa tgaactagac tgggattatg ttgaagaaga
     7441 agtatttaaa aagaatgaag aagatgatgt tcttataaaa acttatgttt ctgaagaaaa
     7501 aaatgtgatg gaatttgtcg aaataataaa gcaaaaagtt ttaggattaa aagattttgg
     7561 tatagatata ggtgaaggaa gtgtatcatt ggaccaagta aatgaagctg attgggcaaa
     7621 tgcatggaaa gcatactaca aaccaacaaa agtaggacaa agggtagtag taaagcctac
     7681 atgggaagat tatgctatgc aagatggtga tttaatcata gagcttgacc ctggaatggc
     7741 atttggtaca ggtacacatg agactactag tatgtgcatt agagaattag aaaagtacgt
     7801 gaataaagat tctaaagtat ttgatattgg atgtggtagt ggaatccttg caatagcagc
     7861 agctaaatta ggagcaaaag aagttgtagc tgtagattta gatgaagtgg ctgtaaaagt
     7921 tgcaaaagaa aatgttcttg aaaataaagt tgaaaaaagt gtatctgtaa tgcatggtaa
     7981 tttaactgat gtaataaaag ataaagcgga tgtaatagtt gcaaatataa tagctgatat
     8041 aataaagata cttgcaaaag atgttcaaaa ctttatgaaa gaagatgcta tattcatatc
     8101 ttcaggtata attttggata aagtagaaga agttaaagaa agcttaattg aaaatggatt
     8161 tgaaatagtt gaagttcaaa aattaggtga atggtcagca atagtttcta agcttaaaaa
     8221 ataagggata tggtgacgta taatggatag attttttgtt gaaaaaaata atataaatct
     8281 acaagataaa acttgcacta tagaaggtga agatgtaaaa catatttcaa aagttttaag
     8341 atgcaaatta ggtgagaaac ttgaaatatg tgacaagaat aataatgagt atatttgtga
     8401 gataatgaat atagataaga gtatagttaa tttagaaatt ttagaaaaag ttgatataaa
     8461 tagagaatca gaacttaaag taaggctata ccagggactt ccaaaagctc caaaaatgga
     8521 aatgatactt caaaaattaa ctgaagtggg tgtagaagaa ataatattag ttcaaactaa
     8581 gagaagtgtt gttaaagttg atgacaaaaa agaagataaa aaatttgaac gttgggaaag
     8641 aataatttat gaagcagcaa agcaaagcaa aagaggtaag ataccaaaat taagaggtgt
     8701 tttaagtttt aaagaagctt tagaagatat gaaaaaaaat aatgtaaata tttgccctta
     8761 tgaaaatgaa agaacagttt caataaaaca tgcactaaaa aaatgtgatt ctaatataga
     8821 tagtgttgga atttttatag gtccagaagg tggattttct gaagaagaaa tagaacaaat
     8881 acaaaaaaat aattgtaatg ttgtatcatt ggggcctaga atattaagaa ctgagacagc
     8941 ttctgttgta gcatcaacaa tagctttgta tgaactaagt gatttaggag gagaaaaata
     9001 aattgaaaaa agtagctttt tatacacttg ggtgtaaagt taatcaatat gaaacagaag
     9061 ctatgctaga actatttgag aaagatggtt atgaacaagt caatagtgaa gagtatgctg
     9121 atgtttatgt tataaataca tgtacagtta ctcatatgag tgatagaaaa tctcgtcaat
     9181 atataagacg agttaaaaag aagaatccag atgcaataat agccgttgta ggatgttatt
     9241 ctcaggtatc tccggaagaa attttggata tagaagaagt aaatttagtc atgggtacaa
     9301 atgatagaag aaagattgtt gaagaaatta aaaagataaa ttcaagtaag aaagttagta
     9361 cagttgatga cataatgaaa gttaaagctt ttgaagaaat agaaatcagc caaactaatg
     9421 gaaagactag agcatttatg aaaatacaag atggatgtga cagattttgt acttattgta
     9481 tcattcctta tgcccgagga cgcgttagaa gtagagatat tgatagtata gtcgatgaag
     9541 tgaaaaaatt ggctaataat ggctataaag aggttgtatt aacaggaata catgttgcct
     9601 cttatggaaa agatttaaag gatagggata ttaaacttct tgatgtaata aaacaaataa
     9661 accagataga aaaaatagaa agaattaggc ttagttctgt tgaaccaatt ttatttacag
     9721 atgaatttgt aaatgaagtt ttaaagatgg ataaagtttg tcctcattac catttatcac
     9781 ttcaaagtgg atgtgatgaa actctaaaaa gaatgaatag acgatacaca actttagagt
     9841 acaaaactat tgtagataga ttgagaagta aaatgccaga tgtggcaata actacagatg
     9901 taatagtagg ttttcctgga gagacaaatg aagagtttaa aaaaacgtac gagtttttaa
     9961 aagaaataga gctttcacaa atgcatattt ttaaatattc accaagaaaa ggaactccag
    10021 ctgcaactat ggaaaatcaa gttgaccctc aaatgaaaca ctttagaagt gaacaacttt
    10081 taaatttaag taaagtaaat tttaataaat ttgcaactaa atttatcggc cgtgagttgg
    10141 atgttttatt tgaacaaaat atagaaggca ataaatatga gggattaact tcaaattata
    10201 taagagttgt tgttgaaagt gacaaaaata tacaaggtca gatattaaaa gtgaaaataa
    10261 atgatgtaaa agatgaatat gtagaaggaa ttttgctata acatgtagaa attataatat
    10321 ttataagata ggaggtggaa agaatggatt gtattttttg taaaatagcg aacggagaaa
    10381 taccttcaac taaagtttat gaagatgata gagtcttagc ttttaatgac ttgaacccag
    10441 ttgctccata tcatatatta gttgtaccta aaaaacatta tgatagttta attgatattc
    10501 ctgataaaga gatggacatt gtgtcacata ttcatgttgt tattaataaa atagctaaag
    10561 agaaaggttt tgaccaaact ggattcagag tcataaataa ttgtggaagt gatggagggc
    10621 aagaagttaa acatttgcac tatcatattt tagctggtaa aaagcttcct aattacgaag
    10681 cgggacaaaa ctaaactaaa ttattgaaaa attgagttcc ttcttgataa aaatacttga
    10741 taaaagtaaa aatttagttt ataataaaca aagtgttagg ttgtaatgca tattatgcaa
    10801 aaagaacata gttacagtcc cagcataatt gcgctattgg agggagggaa aaagaaatgt
    10861 cagaagtaag agttagagag aatgaaacat tagacagcgc tttaagaaga ttcaagcgtc
    10921 aatgtgcaat gtctggcatc atgtcagaag tgagaaaaag agagcactat gataagccaa
    10981 gcgttaagcg taagaaaaaa gctgaagcag caagaagaaa aaacgctaag aaatagtagt
    11041 aaataagaat aaagaggtga aggggatgtc ccttaagcaa aagttacaag aggatttgaa
    11101 atcttcgatg aaaaacaaag atacagtgag aaaatctgta gtaactttaa taagagcttc
    11161 tataaagcag tatgaggttg ataatagggt cgagcttgat gaagatggaa ttatcgatgt
    11221 aatagcaaaa caattaaagc aacgtagaga tgcattagtt gaatttgaaa aagctggaag
    11281 agaagactta ataaaagaaa cagaaggtga aattgaagtt ttaaaagagt acctacctca
    11341 acaattaagc gaagaagagt tagaagaaat tgtaaaatct actatatctg aagtaggggc
    11401 aacttctatg aaggatatgg gaaaaataat gtcagttatc caaccaaaag ttaaaggaag
    11461 agctgacgga aaacttataa ataagttggt aaaacaaaac ttacaataga ttgatatagc
    11521 ctagagagaa atctctaggc tttttatagt ttttgggaat aacgagtcat atttgagcat
    11581 atctataatc aaagaaaatt ttgaaagaag gggaaaaatt tgattatatc agtaaaagag
    11641 attaggaatt ttttgataaa catatttata ccactaataa taggttactt aagtagcatc
    11701 ttaacaatga ttatcagtgg aacagatatt tcaacctatt atttacaact cacaaagcca
    11761 agctttgcac caccagcttt tatctttcct atagtttgga ctatattgta catattgatg
    11821 ggtatatctt catacctaat attaagaaag ggttacaact tacctaaagt aagagatgca
    11881 attttttatt atggattaca acttgtatta aactttattt ggagtatact attttttgga
    11941 tttggactta gatttacagc tttgatagat ataataataa tgattttggt ttctataata
    12001 atgatttcga agttttctaa aatagataag agatctggat acataaattt gatttattta
    12061 atgtggcttg tttatgcagg atttttgaac tattttatat ggtttattaa taaatagaat
    12121 gtaaagattt tgtaaattac cctgtacttt taataatatc ataacaaacg aaaaataaat
    12181 agactattaa aaataatcat tttcatatac aataatatta atttaaatac aataaattta
    12241 taaaaattgt atttaaagat taacatatta aagtattaga attggaaagg cttgaaggga
    12301 gaggttttta tgcaaataat agtatctggg agacaaatga aattaactga cggaataaaa
    12361 ggatacgtag atggaaaatt aagtaggtta gaaaaatacc ttgatcctga atcagaagtg
    12421 aaagtaacag taagtgcgaa aaaggataga caaaaagtgg aggttacaat aattcctatt
    12481 aacggtcaaa taataagagc agaagacgta gaagatgact tatatgcagc aattgatatt
    12541 gtttgtgaca aattaagtag acaagttgtc aaatataaaa ctaaagttaa agacaaagtt
    12601 caaaataata agagcataag atttgaaaat cttgacttta tagataatag cagtgaattt
    12661 gatgattatg attatgatga agaagaagat gaaaatatag taatagaaag aagaaagaaa
    12721 tttaatgtaa aacctatgag ttctgaggag gcgatacttc aaatggaact agtaggacac
    12781 aatttctata tgtttagaaa ccaagataat tttgaaataa acatagtgta taagcgtaaa
    12841 gctggtggat atggtttaat agaacaagat taataaaaat tgtatacatt tagattttat
    12901 ataagaaaaa ataaaaaggt aatcatgttg attttttatc aatatgatta cctttttatt
    12961 gtttaaataa tatattagat attaggtatt tattatataa aaattttaaa actaatctgt
    13021 tacaacatca ataaagtgca taaataccta tttcaagcat aaaattaatt aagggggtaa
    13081 tgcttatgtt agaaatatcc actgatttac aagtaaatca accagttatt actgttacaa
    13141 gtaatacgtt tattagtata gaaaattatc tatcaatatt agaatatgaa gttgatttaa
    13201 taagaataaa gactaaagtt aaaacaatta aaatatctgg ggataaactt tcacttaagt
    13261 atattactga tagtgagata ggtataaaag ggattatcta caatgttgag tatgttgatt
    13321 aggaggagat acacttgata agttttataa ggggatacta tgttatagtt gtggaaggag
    13381 taggactcga acaattttta aatcatctta taagaaatgg gataagtgtt tacaatgtaa
    13441 ctagaataaa aaatactaaa atggagtttc atatagatag acaagatata aaagaattta
    13501 agaatgttta tagaggaagt aagtttgata taaaggtaaa gcaaaaaact ggggtccctt
    13561 ttattatcaa aagagtttat aagcacaaag gtatgtggat ttgtgctctt gtttctttat
    13621 ttttattgat gtctacatca cagtttgtga cagatgtata tatacaatct cctgaaggta
    13681 taaaaaaaga agctcttaga aatgagcttt ataaggttgg agtaagacca ggagtctata
    13741 agaagagtat tgatagaaaa gaagttagag accatatgat gtcaaaattt aatgatgtgg
    13801 catatttatc tatcaatgta aaaggaacaa atatatttgt tactgttaca aaaaaggcag
    13861 aatccctaaa atctacagac caatcaaatt attgtaatgt aattgcttta aaaaatggta
    13921 taatagaaaa ggtaatccct agaagtggta aatcagttat taaatctgga gacatagtcc
    13981 aaaagggaga tgtcttatta aatggagcta ataccaagtc aataccagaa gtatgggcaa
    14041 gtacttttta tgagtccaca aaaaaggcaa gttatgtaga tactgtaaat aaaaaaactg
    14101 gagaaaagaa aaatatatat acactaagtt tttatgataa agagtttaca atgagaaaaa
    14161 atataaaata taaagattat gttgttgaaa ataaagaaaa aaaactatca ataggaaact
    14221 atacttttcc aataaagata aaaacgagta ctttttatga aacaaaaaag gttgaagtaa
    14281 aaaggaataa agaagagtta aaaaaagaat tatctgaaaa agctttaaaa gagttagaat
    14341 acataatccc agcttcggct agaataatag atgtaaaaca taactttaag gttaataaaa
    14401 atatgttaga atatttaata acagttcaaa ctagtgaaaa tattgctaaa atttatcctc
    14461 ttagtaagtc agaagcagaa aggtttataa aggaagaaag taaaccagat gaaggtgaag
    14521 aggaagttcc ttccaatcca gaaaaaagac cattaaatga tataagaaat gagtttgatg
    14581 aggataataa ggataataaa gaccaaaaca attctgatga aaataatagt aatcaaaata
    14641 gtaataatag tcaaaataat aatagcaata acaactaata aaattaaact taaaatctta
    14701 ggaggactaa ttcaattgat aatacaaaag aaatttacaa tagcagatgg taactttgaa
    14761 agagagttat ttggcaactt tgatgaaaat gtaaaattaa tagagaagac tttaaatata
    14821 gatgtaatac ttagagaagg caatataatt ttaattggag aagaaaaaaa tgtagattct
    14881 gcattaaaac ttatgaatga attacaccaa actgtatcaa atggtaagca tttagataag
    14941 cagagtattt cttattcatt atctcttttg cttgaaggaa gtgaacaaaa aataaaagaa
    15001 cttgagggga caatcgtcat aactcaaagg ggaaaagctg ttcaacctaa aactttaggt
    15061 caaaaagaat atataaaact tatagaaaat aatgatataa cttttggtgt tggacctgct
    15121 ggaacaggaa agacttatct tgcggttgct atggcagtaa aagcttttaa aagagatgaa
    15181 gttagtagaa ttatacttac aagaccagct gtggaagcag gagagagtct aggtttttta
    15241 ccaggagatt taaaggacaa agttgaccca tatttaagac cactatatga tgccttattt
    15301 gaaatgttgg gtgctgataa atttaataag tatttagaaa gaggtactat tgaggttgca
    15361 ccacttgcat ttatgagagg tagaacactt gataattcat ttataatatt ggatgaagct
    15421 caaaatacta cttctgaaca aatgaaaatg tttttaacta gacttggatt tggctctaaa
    15481 gctgtagtta caggagatgt aactcaaaca gatttaccac aaaataagaa gagtggtcta
    15541 atacaagcta cagagattct taaaggtgtt ccaggtattg ggtctataat gctaacagat
    15601 agagatgttg taagacatga gttggtacag agaataataa gagcttatga gaagcacgac
    15661 aaaagagaag aatttaaaaa agaagaacgt aaaaaagcga aaatgcagga aaagactttc
    15721 aaaagaaagt aaaagtgtag taatatagat atatagcatt attaatgaat aaaaagtagg
    15781 tgtagtgaat ggatttgata ctagatgata gacaagataa attagaagta agtgaggagt
    15841 taattgaaaa gataaaggat ataatcatag aatgtttaga ttatgaagga tatgatgata
    15901 attatgaagt gagtctttct tttgtagata ataaagagat tcatgaatta aatagagaat
    15961 atagaggagt tgatagagtg actgatgttt tatcatttcc tcttttaagt gatgattttg
    16021 aggatgttga gttagaagaa gaatccctag gtgacatagt agtatctctt gaaagagcct
    16081 tagaacagag tatagagtat aaccatagtt ttgaaagaga agtatgtttt ttaatatgtc
    16141 atagtatgtt tcatctttta ggttatgacc acgatacaga tgaaaataca aaagaaatga
    16201 gagaaaaaga agaacacata ttaaataagt taaacataac aagggagtag tttttatgaa
    16261 accagaaaaa actcgtcaag gtataattaa agcatttaat gcagctatag aagggatatt
    16321 gtatacattt aaatttgaaa gaaatatgaa aatacattat ttaggttcag ttgctgttct
    16381 tataataagt ttgtttttta acttttcaaa attagaaatg ataatgttac ttatgtccat
    16441 atgcttagtt gtagttgctg aaatgtttaa tacagctata gaaaaagctg ttgatttagt
    16501 aactgatgaa tatcatgttc ttgctaagat agcaaaagat gttgcagctg gaggcgttct
    16561 agttgcagca ctaaattcag tagtagttgg atatattttg ttttatgata aattgacaga
    16621 tatatctgga atattaattt ataaaataag agaatcagaa ttacatataa ccttgatatg
    16681 tatattattg gttcttatag ctgttgtagt tgtaaaagca ttgacttcta caggtactcc
    16741 tctaaaagga ggaatgccaa gtggtcatgc tgccttagca tttgctatag caactgcaat
    16801 tacacttatg actgaaagag ttgttgcctc tactcttgca tatataatgg ctgtattagt
    16861 tgcccaaagt agaattgaag gaaaaataca taccttttgg gagactattg ctggagcttt
    16921 gttaggtgtg ttgatagcta tattagtatt tcagcttgga atgttctata attaaaataa
    16981 atgtacataa aatatttaaa ataaaacaaa tctgtgctaa ttaaattttt atgatataat
    17041 gtagagtatt aaataaaaat aacttttaat tattgtatac aggaggagtt gttgtggata
    17101 acatagaatt gttaaggttg gcagaagatg ccagacaaca ttcttatgct ccttactcag
    17161 gttttagagt aggtgcagct cttttaacta agagtggtaa agtttatact ggatgtaata
    17221 tagaatgtgc atcattaggt ggaacaaatt gtgcagaaag aacagctatt tttaaagcaa
    17281 tatcagaggg tgataaggat atctataaaa tagctatagc aagtgataac tcagaaaata
    17341 atgagcaaac atatccatgt ggaatatgca ggcaggtaat tatagagttt ggttctgata
    17401 taaaaataat aacgggatat actaaaggag aggtgtttga gcatagtata aaggatttgt
    17461 tgccaaacta tttctctgga aatgatttaa aataaattta aaagtataaa taaatttaag
    17521 taattaaaac aagaagataa aaagttagga gacagttaat gttcaaatca ggttttgtca
    17581 gtatagtggg tagaccaaat gtaggaaaat ctactttaat gaataatgta gttggagaaa
    17641 aaatagctat aatgagtgat aagcctcaaa ctacaagaaa tacaatacaa gcagtatata
    17701 cagatgaaga aatgcagata gtattcttag atacaccagg tattcacaaa ccaaaaaata
    17761 aattaggtga atttatggta aaggcagcaa cagaagcttt taaaaatgta gatttaatat
    17821 tatttgtagt tgatgattct aaaaaaatag gtcctggaga tagaaagata atagaggatt
    17881 taagaagtgt taagacacca ataattttag ttgtaaacaa aatagaccaa ctagaccaaa
    17941 aagatgagct atttgatata atcaaaatgt atgatagaga aggtattttt aaagaaatag
    18001 taccaatatc agcattaaaa ggaaagaata cggatacctt aataaaagtt atacaaaatt
    18061 atttagaaga agggccaaag tattttccag attatatgat tacagatcag ccagaaagag
    18121 tattaatagc agaacttata agagaaaaag ttcttcacta tcttaatgat gaaatacctc
    18181 atggtgtagc tgttgaaatt gaaaaaatga aagcgagaaa tgataaggaa atagtagatg
    18241 tatctgctgt aatatactgt gaaagagatt cacataaggg aataataata ggaaaaaatg
    18301 gtaggaaact aaaaggaata ggtaagtcag caagacaaga tatagaattg cttttaggtt
    18361 cacaaataaa tcttcaatta tgggttaaag tcaaagaaaa ttggagaaat ttacaaaatt
    18421 atataaataa ttttggatat aatgataaat aaaggtgggt tatatggata ggaaactgga
    18481 tgcttcccaa gatttattat atgaagtaaa atcattaatt gataataata aagtattgga
    18541 gttaagagaa ttaatagagg aatatcatat aattgatata ttcgatataa tggaaaattt
    18601 agaggaagac atgaaaattc aattatttga agtacttcct ttagatatgg catcttctat
    18661 attagaagaa ggtagtgtag agttttttat tagtatttta tctaaactgg atgtagaaca
    18721 ctcgaaaaat atacttgaac ttatgtctct tggagatatg gcagacaagt tgagtgaact
    18781 tgaagaagaa gaaagagaac acataataaa tcttttgaat caagagaatg ccgattatgt
    18841 aaaagagtta ctattctatg atgaagattc tgctggtgga acaatgacca ctggatatat
    18901 ttctataaat aaagatatga cagctcttga ggctatagac catatgagag aagaagctga
    18961 agaagctgaa actatttatt atatatatgt ggttgatgat gaagaaaaac tagttggagt
    19021 attgtcatta agagaattaa taatagctag agacgcaaat atagtagaag atttgatgag
    19081 tgaaaatata atctctgttt atgtagatga agatagagaa gaagcagtaa gattagtatc
    19141 taaatataat ttaatagcaa ttccagtagt agacagacaa gaaaaattaa aaggaataat
    19201 aacagtagat gatataattg atgtaatgga agaagaagca actgaagata tgtataaatt
    19261 tgcaggaagt tcagaacatg aaagagaagt tgctgaaaaa gaaaacccta cattgagaga
    19321 gcaaataata tcagcactta gagggcgatt accttggtta attattacct tagttggagg
    19381 gttgttggct tcgttaatac tttctaactt agactatata atgaatcctg tatatgctcc
    19441 attagtattt ttcatacctg tagtaattgg tatgggtgga aatattggaa ctcaatcatc
    19501 atctgttaca gtaataacat tatctaataa ggatttaaat tttagcaatg tggttagaga
    19561 aggtattgta ggtattatta caggtctttt gtgtagtata ataacaggta ttgtaattta
    19621 ctttgttatg agaaagttag acattgtatt aattgtttca atatcattat ttataaatat
    19681 ggttttaggt gcaacaatag gtgcgtttat gcctgtttta ttaaaaaaaa tggatgcaga
    19741 cccatctaca gtttcatcac caattatatc tacagctctt gatataactg gtatagcagt
    19801 ttactttata ataacaacgg cattattgtc aaaaattgtt taataacttt atgtatgatt
    19861 tatcctcttc ttactaaaaa tataagtata aatgtaagaa gggggttttt taaaaatgaa
    19921 caatatcagt tgggaagttt ttaaaaaaac aggtagtata gatgcatatt tatatttctg
    19981 tgattgcaaa aacatacatg aagaaattga agaagtaagg gagaaaaaag aagacgatga
    20041 taatcctgaa cacccagggg atagtactta aagctataag gtataaggaa agtgacataa
    20101 tacttacact atttacaaga aagcttggaa aagtatctgc aatagcaaaa ggagctaaaa
    20161 aaaataagag ctctttgctt tcatcatcac agttattttc ttatagcaat tttaccttaa
    20221 aaaaacaagg taatatgtac aaggttactc aaagtgaaat aataaaaagt ttttataata
    20281 tatcctatga tatagaggca ttttcttatg ctacatatat tacaaaatta gttgaaaatt
    20341 ctatacttga aaatcagact aataataggc tatttatatt acttgcgcag actctgtatt
    20401 tgtatacaca ggataataca gacaataggt ttataacagc agcattcgag ttaaaatttt
    20461 tagactatat aggatttaaa cctatagtaa ataaatgcat taattgtgaa tgtaaaattt
    20521 tacaaaattc tgtgtttaat atctacgaag gtggtatact atgtagtaag tgtagtaaac
    20581 tttttgataa taatataaaa ttagacttaa caactataag tcttatggaa tatgtattaa
    20641 gaaatgatat tttaacatgt agtaaagcta aagtatcaaa atatataaca catgaacttg
    20701 agaatattct agacaagtat ttgaaagttt atgttgataa tattaatttt aaatcattac
    20761 acgtattgca aagtgtaaaa aataataagg gagtggataa tgatgagtag taatataact
    20821 attgaaatgg ttgattcagt ctttgaaaga gttccaggag caacttatgc agaagcaaaa
    20881 gaagcattag taatgtgcaa tggagatgta attgaggcag taatatattt agaaaataaa
    20941 aaaaatactt gtaattgtaa agcaaaatct gctaaagaaa ctatggaaga agtatttggc
    21001 aaagatggag aaaatataaa gtttcaatta aaagagcttc ttagaaaaag tagtgtagtg
    21061 agaataatag ttgaaaaaga tggaaaaact atgatgaaca ttcctttaac agttggagtt
    21121 gttggattag cttttgtacc attagctact ttagttggat tatcagctgc tgttataagt
    21181 aaatatcgta taaaagttca aaatgaagaa gatggagaaa ttgtagattt aggtgaattg
    21241 aatgaagaaa aacttcacgt tttgaagaat atgataacta atgcagcaaa agatgtgaag
    21301 gatgtagtag ttgacaaaaa agaagatgac aaagatgtaa ctgctgacct tatgaaagaa
    21361 gaatctgaaa agaataaaga agacgaagac gaaaataaat agatataaag tgacttttaa
    21421 aaaatgaaga gaagataatt ataagaatta tcactaacat tgacaaattt tatagaattt
    21481 gatattctaa aagttgtaga agaactatta aaatagcctt tcgtgtagac gagaggcttt
    21541 attgttatag gagggatttt tatgaatttt caagagatga tactggcgtt gcaaaaatac
    21601 tggtcaaagc aaggttgtat catgatgcaa ccttatgata ttgaaaaagg tgcaggaact
    21661 atgaatccta atactttttt aagatcatta ggacctgagc cttggcaagt atgttatgta
    21721 gagccttcaa gaagaccagc agatggaaga tatggtgaaa acccaaacag actataccaa
    21781 catcatcaat tccaagttat actaaaacct tctccagata atatacaaga attatatttg
    21841 gaaagtttaa aagagattgg aattgaccca agtgagcatg acataagatt tgttgaagat
    21901 aactgggaag ctgctacagt tggtgcttgg ggacttggat gggaagtttg gctagatggt
    21961 atggaaataa ctcaatttac gtattttcaa caagtaggta atattgaatg tgagcttgag
    22021 acaggagaaa ttacatatgg attagaaaga ttagctatgt acattcaaga agttgatagt
    22081 gtttatgatt taaaatggaa tgataaaata acttatggtg aagtatttaa taaagctgag
    22141 tatgaaaact caatgtacgc atttgaatta tgtgatgcag atatgttgtt taatctgttt
    22201 gatatatatg aaaaagaagc attaagactt atggaaaatg gccttgtaat accttcatat
    22261 gactatgtgt taaaatgctc tcatgcattt aatacattgg atgcaagagg tgcaatagga
    22321 gttagtcaaa gagcttcatt tataggtaga gttagaaata tggcaaagac tgttgcagaa
    22381 acttttgtaa aacaaagaga agaaatggga ttccctcttt taaaggacgg tgacaaataa
    22441 tgaaaaatta tcttttattt gaaatcggtg ttgaagaaat gccatcaaga tttgtaggca
    22501 gtacactaga acaattaaaa aacaatctta gtaaattatt tgatgaaaat agaatagctt
    22561 ttgataaaat aaatgcgtat gggacaccaa gaagacttac tttagtagtc gaaggattga
    22621 gtgatagaca aaatgattta gaagaggaaa taaagggtcc tgctaagaaa attgctgttg
    22681 atgcagaaaa taatttaaca aaaccagcat taggatttat aaaaagcaaa ggattaagcg
    22741 aatctgacat atattttaaa caagttggaa aagaagaata tatatttgcg actataaagc
    22801 aagatggaaa agaaacttca gaagtactaa aagacatatt accagagact ataaaatctg
    22861 tagtattccc aaaagctatg cgttggggag gaaaaaatct taaatttgca agacctataa
    22921 gatggatagt tgcattgtta aatgataaag tattagagtt tgatttagaa ggaataatat
    22981 cttctaatgt aacaaaaggt catagatttt taggtgaaag tacaatagaa gttgattcta
    23041 tagaagatta ttttgaaaaa ttagaaaaaa attatatagt tttagaccaa aacaagagaa
    23101 aagaaatgat aagaaaacaa tgtatagaag tagcaaattc tttaggtgga gaagttgaat
    23161 ttgatgaaga tttacttgaa gaggtaactt atttagttga atatccaact gctttttatg
    23221 gagaatttga tgtggattat ataaaactac caaaagaagt tgttataact cctatgaaac
    23281 aacatcaaag atattttcca gtacttaaag aaggaaaact gttacctaac tttatagctg
    23341 ttagaaatgg tgattcttac agaatagaca atgttaaagc tggaaatgaa aaagttttag
    23401 aagcaagact tgcagatgct ttatttttct atagagaaga tactaagaga aacttagaaa
    23461 gctacataga aaaattaaaa actgttgtat tccaagtaaa attaggaact atttacgaca
    23521 aaactttaag acttgaaaaa ttaagtgctg atatacttga tagtttaggt ttagtagatg
    23581 aaaaggcaga tactataagg gctgctaaat tatctaaggc agatttagtt actggaatgg
    23641 taggagaatt tgatgaatta caaggtttta tgggaaaaga atacgcaaaa gttggaggag
    23701 aaaatgatgt tgtaagtgaa gctatttttg aacattatct tccaagattt gcaggagata
    23761 tacttcctaa gactaatgca ggagttgcct tatcaatagc tgataagttg gattcaatag
    23821 caggtttctt tgctatagga atacaaccaa caggttctca agacccatat gctttaagaa
    23881 gacaagcttt aggtatactt agtatactta tggacagaaa agttgctgtg gatataaaag
    23941 tacttattga acatgcttta gacaattatt ctgaattaga ttttgataaa gaagaagttg
    24001 ttgagcaaat aatgaatttc ttcaatgaga gagtaaaaaa tctatttaaa gatttaggaa
    24061 taagatatga tgttgttgat gctgttttaa gttctgatat aaatgatgtt tctgatatgc
    24121 atgtgagagc tttagagctt aataattggc tagaaaaaga tgaattagtg gagatgctta
    24181 ctgcatttaa tagagtttct actctagctc aaaaagcaga aagtgataaa attgatgaat
    24241 ctttattaaa agaagatgct gaaattaaac tttacaatga atttaaacaa gtaaaggtaa
    24301 aagttaatga attattaaag gataagaaat atggtattgc tttagatgaa tttgcatctt
    24361 taagaccagt agttgataat ttatttgata cagttatggt tatggataat gatgaagcta
    24421 ttaagaacaa tagacttgct ttacttaaac aagtatatga tactatgcta gaaatttgtg
    24481 atttatctaa aattgtttat aaataaaatt taaactattt ataagaaata aggctgtctc
    24541 aaaatagaga tagtcttatt tttcattgtt gagatgatga aaatatattt gatttcatag
    24601 aaaaagagtg tctcataaac taaaaagttc attttgagac acccatgctt tttgtaagta
    24661 aatataataa attatatttt atacttatca gttattttgt ttaaaatatt atcatttgaa
    24721 tttacccatt cataaaaatt gcctgatgat gcgtccgata ttttataaag catttcagga
    24781 atatcactag ctttaaaatc tccaagtgat tttcccaatc tagtcttacc tatttcaaaa
    24841 ctgcctccaa caaataattc aaatgcatct gtagatatac catcaacatt cttcttttta
    24901 ccacataaac ctatgctacc aacttgatga acaccacatg aatttaaaca tcctgatata
    24961 tataacttag gtgctttatt taaaatttct tcattatttt gtaatctaaa ataatctatg
    25021 atttcatgta acattttctg actattttgt atgcccattt gacaaattgg aacacctatg
    25081 caagatatac tttgttctaa ttgagaatta caactaatag ttttacttat ttctaatatt
    25141 ctttttgctt catttccatt taaatttaaa atatataatc cctctgtcat tcctagtcta
    25201 atcataggat tttcaacatt atctaattct ttaagtaaag tacttaaatc ctttgttaac
    25261 aaaatacctc caactggatg tatatataca gtgtataaac catcctgttt ttgaggtatt
    25321 agtctagaat cctgtatatc tattttaact ccaggtttag agtaatctat ttcttcaata
    25381 ttgagttcaa gactaccatt ttctttttct aatttatagt attctttaaa tttttctaag
    25441 aatagttctt caccaagtga ttcgaccata tatctaaccc tagctctgtt tttattttca
    25501 taatttccat aatccataaa gaatttaatc ataccttcaa cacaatataa ggcatctttc
    25561 ggctttataa gttcatcaag ttctaaagca actttgggtt gtttacctag acctccacct
    25621 aagaaaactc tgaaatatgg ttcatcattt ttcaaagtag caacaaatcc taagtcttgt
    25681 atactacaat gagcagtatc tgtgtaacaa ttagaataag aaacttttaa ttttctaggt
    25741 aaatggtatg tagtagcatt tttgataaaa tatttatcag tagcaactgc atatggagta
    25801 acatcaaaga cttcactagg gtcaactcca gataatggag ataatccgac atttctagga
    25861 taattaccac caccacctct agtgaatata tcatttttta ttccttcttc catgatatca
    25921 acaattgaat ttatagataa atcgtgtaat tgtatagctt gtctagttgt taagtgtatc
    25981 ttatctaagt tgtgtttaat agctatttca tagattttat taagttgata ctgagataaa
    26041 actccagcag atactctaag tcttatcata aaagattttt tgtctctttg agcataaaca
    26101 ccatatccac cagaaaatcc cttatattgc attttattta tttctccatt taaaaatttt
    26161 gaagaatatt ctttaaaatt aggtagttca tttaacaata tctttttaaa attatccatt
    26221 atttcacctc attgttagtc ttgtataaaa at
//
LOCUS       DBGJFF010000052        26249 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.052, whole genome shotgun sequence.
ACCESSION   DBGJFF010000052 DBGJFF010000000
VERSION     DBGJFF010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26249
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.052"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            80..1003
                     /locus_tag="ACOIAF_002086"
     CDS             80..1003
                     /locus_tag="ACOIAF_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGZ2669096.1"
                     /translation="MEIRVLRYFLAIAREESISGAATFLHITQPTLSRQIMELEEELG
                     VKLLVRGNRKITLTNEGRLLHKRAEEIISLVDKVELEFDNPNEIISGDIYIGSGETEA
                     MRLIARTAHHFHQKYPNVRYHLFSGNADDVTEKLDKGLLDFGILIEPANIQKYDYAQI
                     PATDVWGLLMRKDSALASQSTINPKDLCNIPLIISRQTLVDKAISKWLQDSNEKLNII
                     ATYNLVYNASLLVEEGLGYALCLDRLVNTTENSNLCFRPLSPPLEAKLNIIWKKHQIF
                     SKAAEKFLLQIQKDFSSIMLDTSKLDTPKEK"
     gene            complement(1175..2317)
                     /locus_tag="ACOIAF_002087"
     CDS             complement(1175..2317)
                     /locus_tag="ACOIAF_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HGZ2669097.1"
                     /translation="MDFKIEKYLLKKAEELAFITIKHGGEFKLKSYEVPKGGLDVPIK
                     NEVLVKGIKEKTAQDKLNSMSIADAMIYIIGIDSKFKNNAEYEKFLNALSKDIDLDLK
                     SYMGYMSRKYFEIGEHTDSLIYIKAFITMYPDDLDAMYNYAIVCQEIAKQYQKDMDDK
                     AMNAFLLEAMAKLEKVIDVDENFALGYYHLGYHYYNQGQYLKTKLTWEEALKLGLDAD
                     LVAEVQENLGKMDFKVQYEEGYTLVFQGKFKEGLEKLLPLEEEHMDWWNLLFMIALGY
                     KGMGEIEQAKMYLEKILIIKPNQVDTIVELGLCEAYKNNLDKAIEYFEQAAKIKEDPE
                     ILCNLGMAYLNNGDIDDATYYIERAYELNPQDEITIACLRELGINK"
     gene            complement(2335..4089)
                     /locus_tag="ACOIAF_002088"
     CDS             complement(2335..4089)
                     /locus_tag="ACOIAF_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HGZ2669098.1"
                     /translation="MKILLTTLNSKFIHTNLAIRYLKEFVRDLIEVNMKEYTINNDLD
                     YILKDIYKNEYDIILFSTYIWNVGDIVKLCDNLKKIRPNTKIALGGPEVSYDSYEAMK
                     KYDFVDYILYGEGELIFRDLVFHLQGKMEINDVNGLVYRQGNEIIVNKPMELLQDLDE
                     IPSPYENLNPKEYENRIVYYESSRGCPFNCQYCLSSTIPGIRYFSLDRIKSDLKALID
                     ARVSQIKFIDRTFNANRKVAMEIMDFLMKNDNGYTTYHFEVTAYLIDDKMLEFLADCK
                     EGLFQFEIGVQSTNEKTLDAVGRRDDFKKLSHVVQTVASYRNIHQHLDLIAGLPYEDY
                     KSFENSFNDVFNLGIEHLQLGFLKMIKGTGMRKVADEHGFKYKDYAPYEFLYNNYISY
                     EETLKLKDIEDILERYYNSKNFVLSMRYIIGRFYKKSPFKFFEVFAKYFDENGYFDLA
                     QGKNQLYKILMDFYNEKINIDNDVFNDILKYDYISLGKTSNIPQFFNKLDVDDFKNRC
                     HVFLQDNDNLSTYLPSFVDKPAKHIIKYVHFEPFRFNVVDLKNDINTEIREVENVVLF
                     EYDDKKIFEKSKTHKVEI"
     gene            4225..4431
                     /locus_tag="ACOIAF_002089"
     CDS             4225..4431
                     /locus_tag="ACOIAF_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669099.1"
                     /translation="MELIEKVSINSLSKRDLLLIIKSLEYTNENTQISDFIDLRNNIV
                     KELCFLTDTKEKDFLDYLEKNSTL"
     gene            complement(4436..5359)
                     /locus_tag="ACOIAF_002090"
     CDS             complement(4436..5359)
                     /locus_tag="ACOIAF_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669100.1"
                     /translation="MNDELLKKVYTSSFSSASRALKDLTIINTKSLIDDKTQRCVYID
                     ENRLRDELIYYRFYGEKIGNINFLNILLPLILSNTNIQKSEDEVIKLIKKYVIYFKKE
                     ELLFDYLLGSVVYNSIMHNLINNSKIEYVELLQSIKDKIIGFSIELDKSDVVKFQMAR
                     IKAIQLIDKYIDLKSEDYDEESVLLNVLNVLYDVYMEDRTVENEGINSIKKSILSILG
                     EDSKLNEDNIDFIFSMSEYVVKLRKYKIGVKAYNKSIDPRSLIRLEEGNTIVDPIFNQ
                     ITVMSKTFNDNILSIKINSKSGIYILKFKKV"
     gene            complement(5613..7313)
                     /gene="argS"
                     /locus_tag="ACOIAF_002091"
     CDS             complement(5613..7313)
                     /gene="argS"
                     /locus_tag="ACOIAF_002091"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HGZ2669101.1"
                     /translation="MQDFKVAISNCLKEKIEDLSKEEIEALIEVPPNKDMGDYAFPCF
                     KLAKVFRKAPNMIASELAESIEPSGEITKVIQLGGYVNFFVNKSQLAETVIKKVLDEK
                     ENYGHSDFGKDKTVIVEYSSPNIAKPFHIGHIRTTVIGNALYKIYDSQGYKTIRINHL
                     GDYGTQFGKLIVAFKKWGEKEVVESNPIPELLKLYVRFHDEAEQHPEMEDEARAWFNK
                     LENGDEEAQELWQWFRNESLKEFNRVYKLLDIEFDSLAGESFYSDKMNRVIELLEEKN
                     LLKESKGARIVDLEEYKMPPALITKNDGSTLYMTRDLAAAIYRKETYDFDKCIYVVGS
                     QQNLHFQQWFKVIELMGYDWAKDLIHVGFGMVALEEGTMSTRKGRVVFLEDALNQAID
                     KTKEIILAKNPNAKNVDEISKQVGVGAVVFQELSNSRIKDYTFSWERTLSFDGETGPY
                     VQYTHARCCAVLRKAEVEVTSDIDYSLLADEDSAEVLRVIESFNKNILLALKKNEPHI
                     VTRFMLDLAQAFNKFYHDNPILVENLEIRKARLALVLATKQTLENSLKLLGMHAPERM
                     "
     gene            complement(7725..8138)
                     /locus_tag="ACOIAF_002092"
     CDS             complement(7725..8138)
                     /locus_tag="ACOIAF_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HGZ2669102.1"
                     /translation="MILVSACLIGINCKYSGDNNDNEKVKEYLKGKEFTLVCPEQLGG
                     LSTPRPPAEIVDGKVITKDNKDVTENFVRGANETLKIANLYVCSEAILKEGSPSCGCN
                     LIYDGSFSGKKIPGKGITARLLEEEGIKVKSEKDL"
     gene            complement(8204..10582)
                     /locus_tag="ACOIAF_002093"
     CDS             complement(8204..10582)
                     /locus_tag="ACOIAF_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HGZ2669103.1"
                     /translation="MNEKSLRVLEYNKIIDLLKKKASSSLGLKYIENLVPNTDFVEVK
                     SMLEETSEAQSIIIKRGSVGLEGIHDIEDKVKRAYIGASLDPGSLIMIADTLRVARRL
                     RNSLSSSDEEDFNYPIIQSLSNSLYVYKDIEDQIYNAIISEVEISDNASSTLRDIRRR
                     IAQKNQSIRSKLNSIISSTTYQKYLQDAIISLRGDRFVVPVKSEYRSQVAGIVHDQSS
                     SGATLFIEPMTIVEMNNELRQLKLGEQEEIERILSELSAMVGEVSEDLISNQEILGRL
                     DFAFSKGKLSIQMRGIEPTLNEDKYLNIKNGRHPLLDKKKVVANTIYLGRDFHTLVIT
                     GPNTGGKTVTIKTVGLFALMTQSGLHIPADYGSSMCVYDNVFADIGDEQSIEQSLSTF
                     SSHMTNIVSILQNVTADSLVIFDELGAGTDPVEGAALAIAVLEDINSVGAKCIATTHY
                     SELKNYALTKPGVENAAVEFDIETLSPTYKLLIGVPGKSNAFEISRKLGLSDYVISRA
                     KEYINTENIALEDVLQNVEKNRIKAVEDREEAERLKEEIEKLKVEYDEKLEKLVSQRD
                     KMIEKAKSEAFSIIRQAKEEVDIIIKELRSLEQERASKEKNRKIEELRKELTSSMGSL
                     QPTVKSMIVPKVSNKEIKDLKPGEEVKVITLNQNGSVVSVDKKRKEAVVQIGIMKMTL
                     PFKSLQKTRKDVSTNVTKSTRNIIRSKSGSVKNEVDLRGLNLEEAIMEVEKYLDDAYV
                     AGLESVTVIHGIGTGVLKAGLQDILRRNRHVKSQRGGQYGEGGAGVTIVKLK"
     gene            10901..12352
                     /locus_tag="ACOIAF_002094"
     CDS             10901..12352
                     /locus_tag="ACOIAF_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HGZ2669104.1"
                     /translation="MGNVLEGLKPESVFKNFEKISQIPRGSGNEKGISDFLLSFGKNL
                     GLETIQDESLNIIIRKPATKGYENCPGVVLQGHMDMVCEKEKNVEHDFLKDPIKLRID
                     GDMIYATGTTLGADNGIAVAMGMAILEDNTLEHPALEVLVTVNEEDGMNGADALDPSL
                     IKGQYILNMDSEEEGYLLVSCAGGKTCVVSLPVEYKEVKGDKQGLLVEVTGLLGGHSG
                     MEIVLQRANANKAIARVLSVLNVDYELASVDGGTKHNAIPREAKCVIAVNKADVESAK
                     KQINDILTAFKHEFTTSDPGMTYSVAETSVDKVLTKDCKEKVVQMSCLTPHGVQSVSL
                     DIEGLVESSTNFAIIETKESTIEFLTSVRSSVMSIRDEIADRIRLLAQALGANYDLIA
                     QYPAWEFKKGSKLEKICSETYEKLTGKVPTVMALHAGLECGLLLDKLPHAEAISIGPD
                     MFDVHTPNEHVSIPSVANVWDYVIEILKSMNQY"
     gene            complement(12556..15069)
                     /locus_tag="ACOIAF_002095"
     CDS             complement(12556..15069)
                     /locus_tag="ACOIAF_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HGZ2669105.1"
                     /translation="MEDCVVNLKKLLLLIIVITSIFTSCIFSFGINAIGNDNRTIRVA
                     YPIQKGLTEIDEQGNYFGYTYEYLLEIAQYTGWNYEFVQVPGEINESITKLMKMLENG
                     EIDIMGGMLYNEPMQKIYNYAGNNYGVANTVLQALYENTDIDNIMSSQDVKKIRIAVI
                     ENSDTRLKELDEYCKMNLMSPELVYCKTNDEQLEVLKSGKADVLLNVSMNPIEGVRTV
                     AKFSSKPFYFITPKGSTDLTQKLNSAINNIEQKDPYFSTTLYEKYFTPENNQMFLSDE
                     ETNYIKDTEKLKVGVLLNKPPFQNADKDTGELRGISIDLLNHICDKTGLEFELVPVKS
                     QEDLEDMAKKHEIDIVAGMIYDYEYSREHNIAMSRPFLSTQYTMMINNKLNENSLNGK
                     RIALTKGVNYYGQFEENIVWYDNLEECIEAVNKGYADYTYGEGYSMQYYMNQPKYKNV
                     RLVPQTYKAKSICFGIIKKDKQELLNIFNKAIISISSEKMQSIVYQNTTYRQDFSLLY
                     IMQQNPIETIFIVASLLICIIGFMAFGLHTRTSMNEKISLELKKHTQLYEMTSEYFFE
                     YDYKQDKLIISMPEKGIETYYGLDKNDKESENGNIENERDKIRKEYNKKFFDVIKSQE
                     NGTTEMYSIMLDDTFHWIRVTSKIIYNDRKIPMYVIGKICNIDSEKEEKNRLLDKAQR
                     DSLTNIYNAAYIRKLTAQNLLNLSTEKNGALLIIDIDYFKSINDTYGHLLGDKVLIDV
                     AKVLDESFAKNDIVGRLGGDEFSVYINDIINKEELINKCNDVYNHIHLIKLPNGESLS
                     ISMGVVIAKPGQEYDELYQIADNALYDSKRQGRNRFKIV"
     gene            complement(15292..16113)
                     /locus_tag="ACOIAF_002096"
     CDS             complement(15292..16113)
                     /locus_tag="ACOIAF_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HGZ2669106.1"
                     /translation="MIDINLEKCVGCGMCESDCLVNAIKVKDDKAKVKNILCINCGHC
                     MAICPTDAIEMQGFDKNEVIEYNRESFELEPEKLLNFIKFRRSIRQYKDIEVEEEKIK
                     NIVEAGRYTPTGGNRQPIRYILVKEKLKEVKELAIQGLYNLALDTDDNDPVRSMYKNT
                     FKKMYKRYKENGNDSLFYDAPLLMVVVGDMSLGGSAYVDGGLAASNMELMAYSQGLGI
                     CYNGFFVMASNVEPKIKELLGMSENEAVITSFILGYPDVKYKRTVNRNTAKFEVR"
     gene            complement(16631..17107)
                     /locus_tag="ACOIAF_002097"
     CDS             complement(16631..17107)
                     /locus_tag="ACOIAF_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HGZ2669107.1"
                     /translation="MKLTTICYIEKDDKTLMLYRNKKKDDIHEGKYVGVGGKFEQGET
                     PEECVIREVKEETGLTLKSLSYKGLITFPKFKDEEDWYMFLYFSDEFEGELSEKDLND
                     CKEGNLIWVDNDKIFDLNMWEGDRLFLNWAKTGNIFSAKIVYDNGKLKDYNVSFLD"
     gene            complement(17141..17719)
                     /locus_tag="ACOIAF_002098"
     CDS             complement(17141..17719)
                     /locus_tag="ACOIAF_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HGZ2669108.1"
                     /translation="MREEALEILFKYLETDRMRKHCYAVEAVMRELAKRLEPEKEEEW
                     AVAGLLHDLDSDIVGRKPGEICEGHATTTVELLKKENFGDEEMYRAILGHHDGMGVTR
                     TSLMEKAIYAADPITGFITAIALVYPDKKLTSVKTKSVIKRMKETRFASNVNRDAMRS
                     IEDIGIPFDEFAELSLNAMKNISDVLESAENN"
     gene            complement(17823..20423)
                     /locus_tag="ACOIAF_002099"
     CDS             complement(17823..20423)
                     /locus_tag="ACOIAF_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HGZ2669109.1"
                     /translation="MKKIELLAPVGSFDSLKAAVQNGANAVYLGGKDFSARASANNFD
                     RNELIEAVKYSHIRGVQVFVTVNTLIKQYEIEDFVEYVKFLYDIDIDALIVQDIGMAR
                     LIKKLLPDFELHASTQMVAHSLEDVKYLQSVGFDRVVLARELNVDEIKWICENTTVDI
                     EVFVHGALCVCYSGQCLMSSMIGNRSGNRGRCAQPCRQKYELIDINTGEVVKSNGDYL
                     LSPRDLSTIEDLDKIIEAGVLSLKIEGRMKKPEYVATVVSGYREAINEFVDKKKISIS
                     EEIINNLYTIFNRKFTKGYILGEVGKDIMNSNRPNNQGLYIGKVLDYNRKNKRLRVML
                     ENTLKKGDGINLGGGTIGRIIKDGEITTIAHKGDLIELDFVGEAKRNQLIYKTSDSEL
                     MDRVQATFQQDKEFIKTNIRAKVSIKLGKKPELFISDFCGNKSVVEGDKIVEKAIKVA
                     LSKEKIETQIQKLGNTPYKLKEIEIDLDEGVSMPISVLNQMRRDCIELLDEERIKIKD
                     RKFKKNRLKYQPNRVNKQKKLDTVPKIRVKVKNLEQLEAVLNYNVDLIYYEDINTLSK
                     ALQMVSSISIDSNISIDSSISMDLDTSSSLDTPKELMGSNTNNIDTLSNTSKIDTLNK
                     NHYNTAVTDKKLIYSAPRIVRNKEYKQFEILDNIKGINKVQIGNWGSMEYFKKKDLNI
                     DCYLNAFNSESINHFKEEGASTVCLSQELNLNEIKQTLKYTDVEIEAVIYGYAPMMVT
                     EYCPMGVVVRDCKKDKRVAKCKESSYALRDFTDASYRITQDIFCRSTIYNSKVTCMLD
                     NLYELEEIGIDVFRIDFTVEDSEMVKKVIEAHIEVLSNNYKLGKKATDLYKKLDEIGT
                     VAGHYYKGVE"
     gene            complement(20876..21559)
                     /locus_tag="ACOIAF_002100"
     CDS             complement(20876..21559)
                     /locus_tag="ACOIAF_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HGZ2669110.1"
                     /translation="MTSKSQMSESFFLCSLLAITGGFLDIYTYVVRGKVFANAQTGNI
                     VLFGLNIAEGNVKESFYYLIPILAFMLGVFIAEKIRKYFTNNKTKIHWRQVIIIIEMI
                     TILIVSFIPRGRFNMFVNVAISFICSMQVESFRKVNGNSFATTMCTGNLRSATETLFH
                     YIHTKNIFMIKKSLQYYAIIIFFISGAILGTFFTKIFVEKSILICFFILAVVFGIMFI
                     NKDDSFNEN"
     gene            complement(21779..22369)
                     /gene="zapA"
                     /locus_tag="ACOIAF_002101"
     CDS             complement(21779..22369)
                     /gene="zapA"
                     /locus_tag="ACOIAF_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HGZ2669111.1"
                     /translation="MNKVMVKIHGAEYPMVGDKSEKFMISIADFVDKEMDKITRQNPK
                     LSLSVAAILTALNISDLLFECSDENEKLIKANEELSKKVGASNEELQLEIKSLKLTIA
                     EKEAENREAETKMKELIEIIENKKQEIFELSNTTEGSRAELDAYKNKIEELSTQLEEA
                     NERATIAENLASEFQNKAYDLQLKCTGLNNDAKNVE"
     gene            complement(22394..24787)
                     /gene="pheT"
                     /locus_tag="ACOIAF_002102"
     CDS             complement(22394..24787)
                     /gene="pheT"
                     /locus_tag="ACOIAF_002102"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HGZ2669112.1"
                     /translation="MLVSLKWLRDYVDIDMDVKEFADKMTMTGTKVETIDYYGEEIEN
                     ILVGKILEIKQHPNADKLVVTKVDIGDKVVQIVTGATNISEGDYIPVAVNGSKLPGGV
                     EIKQTDFRGELSDGMMCSAAELGIDEHYIEEYKRGGIYILDHEDSYELGKDIKDVLGL
                     KDALIDFELTSNRPDCKCMMGIAREAAATIGTKVKYPEIEVKESDEEIDFKVEIDNPD
                     LCRRYVARMVTDVKIEPSPYWMQRRLTEAGVRPISNIVDITNFVMLELGQPLHAFDIN
                     QVETGRIVVRNAKDGEKLVTLDDVERTLDKDMLVITNGEKSLGLAGVMGGANSEITSN
                     TKTVLFESANFKPENIRMTAKKVGIRSEASSRNEKDLDPNLAEIAANRAAQLVEMLGA
                     GKVLKGVVDVYPNKPEPKKLVVNPQRINHLLGVDVPMEQFVGILESLEFKCNLVANDK
                     LEIDVPSFRTDMEQEADVWEEIARIYGFENIPSVQLEGNTTAGIKTSKQKFMDALKDN
                     STAVGLNEILTYSFVSPKGVDKIRVPEGNAKRNFVKLLNPLGEETSVMRTTLIPNMLD
                     VLSTNVSHKIEEVSAFECGHIFIPQDSELPKEENRMCVGMYGKDVDFFALKGTIETIL
                     VNVGFKGYEIEPQDNNTTFHPGRCAKIVYNNKYVGTLGELHPDVIENYNLGQRVYVAE
                     IDIDFVFDNSDRTKNYVPLPKYPSTSRDIALIVKDDVFVKQIEDIIKENGQGLVESYK
                     LFDVYKGSQIEAGYKSIAYSITYRSKDKTLTDEDVAKVHDKILSELSEKLNANLRSN"
     gene            complement(24804..25823)
                     /gene="pheS"
                     /locus_tag="ACOIAF_002103"
     CDS             complement(24804..25823)
                     /gene="pheS"
                     /locus_tag="ACOIAF_002103"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HGZ2669113.1"
                     /translation="MQEKLLALREAALAEIKEAQSIESVESLRVKYLGKKGEITAILK
                     EMGKLSAEERPVVGKVANEVRENIELSINSKKEEINAIEKERKLKEEVIDVTQPGKVL
                     KVGKKHPITQIIDEVTDIFIGMGFSIAEGPEVETVENNFDALNAPKDHPSRDMSDTFY
                     INDGVLLRTQTSPVQVRTMRSQELPIKVIAPGRCFRSDSPDATHSPMFHQIEGLVVGK
                     DVTMAEFKGTMDIFVEKLFGSDIKTKFRPHNFPFTEPSAEVDVTCFKCGGKGCPMCKY
                     EGWIEILGSGMVHPNVLRNCGIDPEVYSGFAFGVGVERLAMLKYEIDDIRLLFENDMR
                     FLNQF"
     misc_binding    complement(25873..26107)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         8214 a         4786 c         2896 g        10353 t
ORIGIN      
        1 aaatacttaa ctatacttaa aaagcatata aattgatata attatataat aaataagtta
       61 atattgagga gggataatta tggaaataag agtattaaga tattttttag caattgcaag
      121 agaagaaagt atatctggtg cggcaacctt tttacatata acacaaccaa cactttctag
      181 acaaattatg gaacttgaag aagagttggg agttaaatta ttggttcgag gaaatagaaa
      241 aatcacctta acaaatgaag gtaggctttt acataaaaga gcagaagaaa ttatatcatt
      301 agttgataag gttgaattag aatttgacaa tcccaatgag attattagtg gtgatatata
      361 tataggaagt ggtgaaactg aagctatgcg acttatagct agaactgccc atcattttca
      421 tcaaaaatac cccaatgtaa gatatcacct ttttagtggg aatgctgatg atgtgactga
      481 aaaattagac aagggactgc ttgattttgg tattttaatt gaacctgcaa atatacagaa
      541 gtatgattat gcacaaattc ctgctactga tgtatggggt ttactaatgc gtaaagatag
      601 tgcccttgca tcacaaagca caataaatcc aaaagattta tgcaatatac ctcttattat
      661 ttcaagacag acacttgttg ataaagcaat ttctaaatgg cttcaagaca gtaatgaaaa
      721 actaaacatt attgcaacat acaatttagt ttataacgct tctcttttgg tagaagaagg
      781 tctgggctac gctctgtgtt tagatagact cgtcaatact acagaaaaca gtaatctttg
      841 ttttagacct ctatctcctc ctttagaagc taaattaaat attatatgga aaaaacatca
      901 aatattttca aaggcagcag aaaaattttt acttcaaata caaaaagatt tttcttctat
      961 catgttggat acatctaagt tggatacacc taaagaaaaa taaatcataa taaaaagtga
     1021 gagtgtctca aaatgaactt tgcactgtat aaggcactct tttttataca aagtaaaata
     1081 tatgttcatc atttcagcac taaaaaagag gcttatctct attttgagat agccccactt
     1141 aaccaataaa attaaataca acttaacaat atccttactt atttattcca agttctctta
     1201 gacaagcaat agtaatttca tcttgtggat ttaattcata agctctttct atatagtaag
     1261 ttgcatcatc aatatcacca ttatttaaat aagccattcc taaattgcaa agtatttctg
     1321 ggtcttcctt tatcttagcc gcttgttcaa aatactctat tgctttatct aaattattct
     1381 tataagcctc acaaagcccc aattccacta tagtatccac ctgattaggc tttattatca
     1441 atatcttctc aagatacatc ttagcctgtt ctatttcgcc cataccctta tatccaagtg
     1501 ctatcataaa cagcaagttc caccaatcca tatgttcttc ttctaatgga agtaacttct
     1561 ctaatccttc cttaaactta ccttggaata caagtgtata tccttcttca tattgaactt
     1621 taaagtccat cttgcccaga ttctcttgta cctcagctac taaatcagca tctaacccta
     1681 gctttaacgc ttcttcccaa gtcagcttag tctttaaata ttgaccttga ttataataat
     1741 ggtagcctaa gtgataataa cctaatgcaa aattctcatc aacatcaatt accttttcaa
     1801 gcttagccat ggcttcaagt aagaatgcat tcattgcctt atcatccata tctttttgat
     1861 actgtttagc tatctcttgg caaacaatcg catagttata cattgcatct aaatcatctg
     1921 gatacattgt tataaatgct tttatatata ttaaagaatc tgtatgttca ccaatctcaa
     1981 agtattttct cgacatatat cccatatacg atttcaaatc caaatcaata tcttttgata
     2041 aagcatttaa aaacttctca tactctgcat tgtttttaaa cttactatct attccaatta
     2101 tatatatcat tgcatctgct atagacattg aatttaactt atcttgagca gtcttttctt
     2161 ttatcccttt tactaatacc tcatttttta taggtacatc taatccacct tttggcactt
     2221 catagctttt taatttaaac tctccaccat gttttatagt tataaaagct aattcttctg
     2281 ctttttttag caaatatttt tctatcttaa aatccatttt atcctcctaa atacttaaat
     2341 ctctacttta tgagttttag atttctcaaa tattttttta tcatcatact caaataagac
     2401 tacattttct acttctctta tttctgtatt tatatcattt tttaaatcca caacattaaa
     2461 tctaaatggc tcaaaatgaa catattttat tatatgttta gctggtttat caacaaaact
     2521 aggtaaatat gttgataagt tatcattatc ttgtaaaaat acatggcatc tattcttaaa
     2581 atcatccaca tctagcttat taaaaaactg tggtatattt gatgttttac ccagtgatat
     2641 atagtcatat tttaatatat cgttgaatac atcattatct atgttgattt tttcattata
     2701 gaaatccatc aatattttat ataactgatt ctttccttgt gccaaatcaa agtagccatt
     2761 ttcatcaaaa tacttggcaa aaacctcaaa gaatttaaaa gggctttttt tataaaatct
     2821 gcctataata tatctcatag ataacacaaa gtttttagaa ttgtagtatc tctccagtat
     2881 atcttctata tcctttagct tcaaagtttc ttcatagctt atataattat tgtataaaaa
     2941 ctcatatgga gcataatcct tatatttaaa accatgctca tctgcaactt ttctcatacc
     3001 tgtcccttta atcatcttta aaaagcctaa ttgcagatgc tctataccta gattaaatac
     3061 atcattaaaa gaattttcaa agctcttata atcttcataa ggcaatcctg caattaagtc
     3121 taagtgctga tgtatatttc tataagatgc tacagtttgt acaacatgag acaacttttt
     3181 aaaatcatcc cttctaccta ctgcatcaag tgttttttca ttcgttgact gaacacctat
     3241 ttcaaattga aaaagacctt ctttacaatc agccaaaaac tcaagcatct tatcatctat
     3301 caaatacgct gtaacttcaa aatgataagt tgtatatcca ttatcatttt tcataaggaa
     3361 atccatgatt tccatagcca cttttctatt tgcattgaat gtcctatcta taaactttat
     3421 ttgtgatact cttgcatcta ttaaagcttt taaatcactc tttatcctgt ctaaactaaa
     3481 ataccttata cctggaatag tagatgataa acagtattgg cagttaaaag ggcaacctct
     3541 tgatgattca taatacacta ttctattttc atattctttt ggattcaagt tttcataagg
     3601 actaggaatc tcatccaaat cttgaagtaa ttccataggc ttattcacta ttatctcatt
     3661 gccttgtcta tatacaagtc catttacatc atttatttcc atcttccctt gcaagtgaaa
     3721 tactaagtct ctaaaaatta attctccctc tccataaagt atataatcca caaaatcata
     3781 ctttttcatt gcctcataac tatcataact cacttccggt ccgcccaaag ctattttagt
     3841 attaggtcta atttttttta gattatcaca aagtttaact atatctccca cattccagat
     3901 atatgttgag aataatatta tatcatattc atttttatat atatccttta aaatatagtc
     3961 caaatcgtta tttatcgtgt attctttcat attaacttct atcaaatctc ttacaaattc
     4021 ttttagatat cttatagcca aatttgtatg aataaacttg gaatttaatg ttgttaataa
     4081 aattttcaaa tatttctcac ctctttttaa gattatactg tctttttgta ccccttatag
     4141 gttaaaatag tatatgtaaa gtctaaataa tatctatata attaatttat taatttcaag
     4201 taatgtgagg aaaggaagtt ttttatggaa ttaattgaaa aagtatctat caactcttta
     4261 tcaaaaagag atttactact tattataaag tctcttgaat acactaatga aaatactcaa
     4321 attagtgatt tcattgacct taggaataat atagtaaagg aattatgttt tttaactgac
     4381 actaaggaaa aagatttttt agattatcta gaaaaaaata gcacactcta ataacctaaa
     4441 cctttttaaa ctttaatata taaatacctg atttagaatt tatttttatg cttaatatat
     4501 tatcattaaa ggttttcgac ataactgtta tttgattaaa tatagggtct actatagtat
     4561 taccttcttc aagtcttata agacttcttg ggtctatgct tttattatat gccttaactc
     4621 caattttata ttttctcaat ttaacaacat attcggacat agaaaaaata aaatctatat
     4681 tatcttcatt taatttacta tcttcaccca aaatggataa tatagatttt tttatgctat
     4741 taattccttc attttctaca gttctatctt ccatatatac atcatataga acatttaaaa
     4801 catttaataa aacactttct tcatcataat cttcagattt caaatcaatg tacttatcaa
     4861 ttaattgtat tgctttaatc cttgccatct gaaactttac aacatctgat ttgtctaatt
     4921 ctatagaaaa tcctattatc ttgtctttaa tactctgtag taattcgaca tactctattt
     4981 tactattatt tataagattg tgcataatgg aattatacac cacagaacct aataaataat
     5041 caaataaaag ttcttctttt ttaaaataaa taacatactt ttttattagt ttaataacct
     5101 catcttcact tttttgaata ttagtatttg ataaaattag tggtaataaa atatttaaaa
     5161 aattgatatt tcctattttt tctccataaa atctatagta aataagttca tctcttagtc
     5221 tattttcgtc tatatacaca catctttgcg ttttatcatc tattaaggat tttgtattta
     5281 ttatagttaa atctttaaga gccctacttg cactactaaa agaagatgtg tagacttttt
     5341 ttagtaattc gtcattcata aatatatcct ttctgaaaaa ttgaatgtaa ataaattgtt
     5401 attaaaaaaa aagagacagc ccgtttaaga agctgtcata tataggggga tagtagtgta
     5461 caaaaatcat tgtaatctac tatctatata ttattatacg atactacttt tttatatgta
     5521 aagcatcagt tttcgacatc cctatatttt acagttatac aaattgcgta ctaagttcaa
     5581 ttttattctt tttatttcaa cattttatca tgctacattc tttcaggagc gtgcattccc
     5641 aacaatttca gagaattttc taaagtttgt tttgttgcta aaactaatgc aagtcttgct
     5701 tttcttattt ccaaattttc tactaaaatt ggattgtcat gataaaattt attaaacgct
     5761 tgagctaaat ctagcataaa tctagtcact atatgtggct catttttctt tagagcaagt
     5821 aaaatattct tattgaatga ttctataact ctaagaactt ctgcactatc ttcatcagct
     5881 aatagactat aatctatatc agaagtcact tctacttctg cttttcttaa aactgcacag
     5941 catctagcat gtgtatactg aacatatggt ccagtctcac catcaaaact aagcgttctt
     6001 tcccaagaga aggtatagtc tttaattcta ctattagata actcttggaa tactactgcc
     6061 ccaacaccta cttgcttaga tatttcatcc acatttttag catttgggtt tttagctagt
     6121 ataatttcct tagttttatc tatagcttga tttaaagcat cttctaaaaa tactactctt
     6181 ccttttctag ttgacatagt accttcttca agtgcaacca taccaaatcc aacatgaatt
     6241 aaatcttttg cccaatcata tcccataagc tcaattacct taaaccattg ttggaaatgt
     6301 aagttttgtt gagaaccaac tacatatata cacttgtcaa aatcataagt ttcttttcta
     6361 tatattgcag cagctaaatc tcttgtcata tataacgttg aaccatcatt cttagtaata
     6421 agtgctggtg gcattttata ctcttctaaa tcaacaattc ttgctccttt agactctttt
     6481 aaaagatttt tctcttctaa tagttcgata actctgttca ttttatcaga ataaaaactc
     6541 tctcctgcta aagaatcaaa ttctatatct aaaagtttat acactctgtt aaactctttt
     6601 aaactctcat ttctaaacca ttgccataat tcttgtgctt cttcgtcacc attttctaat
     6661 ttattaaacc atgctcttgc ttcatcttcc atttctgggt gttgttcagc ttcatcatgg
     6721 aatcttacgt ataattttag caattctgga attggattag actctactac ttctttttct
     6781 ccccactttt taaaggccac tattaactta ccaaactgag ttccatagtc acctaaatga
     6841 tttattctta tagtcttgta tccttgcgaa tcatatatct tatataaagc attacctata
     6901 actgtagttc ttatgtgccc tatatggaaa ggttttgcta tatttggaga agaatattca
     6961 actattacag ttttatcttt tccaaaatca ctatgtccgt aattttcttt ttcatctaaa
     7021 acttttttaa ttacagtttc tgctaattga gatttattta caaagaaatt tacatatccg
     7081 cctaattgta ttacttttgt tatctctcca cttggttcta tactttccgc caattcactt
     7141 gctatcatgt ttggagcttt tctaaacact ttggctaatt taaagcatgg aaaagcatag
     7201 tcccccatat ccttatttgg tggaacttct ataagtgcct ctatctcttc tttcgataaa
     7261 tcttctattt tttctttaag gcaatttgat attgccacct taaaatcttg cattctgttg
     7321 ctcctctcta cattgttcct ggtttatatt gtgcttatct ctttatgtta aatcaaattt
     7381 cgtgtttttt caagtatttc attacagtgt aaaacacttt agtaattatg ttttctgtat
     7441 atattaaaaa tactataatt gcagttttta atcacactat tattgatttt ttataagact
     7501 tttttctatt ttattgtttc ttgtactatc cagttgactt tatatacttt ttttaatctt
     7561 ttatctattt tcatacgtca atgctaattt tatatacttt tcttttaatc ttttattaat
     7621 tttcatacat caatgttaac tttatatacg tttttttaat cttttatcta ttttcataca
     7681 tcaatgtcag ctttatatac tttttttact cttttatcta ttttctataa atctttttca
     7741 ctcttaactt ttattccttc ttcttctaat agccttgctg ttataccttt ccctggtatc
     7801 tttttacctg aaaaactacc atcatatatt agattacatc cacaagaggg gctaccctct
     7861 tttagtattg cttcgctaca tacatataaa tttgctattt ttagagtttc attagcccct
     7921 cttacaaagt tttctgttac atccttatta tctttagtaa ttacttttcc atcaactatc
     7981 tcagcaggtg gtcttggtgt agatagacca cctaactgct ctgggcatac aagtgtaaat
     8041 tctttgcctt ttaaatattc cttgactttt tcattatcat tattatcgcc actatattta
     8101 caatttattc ctattaaaca tgcagatacc aatatcataa aaatgtgctc ctatacaaat
     8161 tatatatatt ctcatacaaa ttactaatgt attcttatac aaattatttt aatttaacaa
     8221 ttgttactcc tgctccacct tctccatatt gaccacctct ttgtgatttt acatgtctat
     8281 ttcttcttaa aatgtcttgt agccctgctt ttaatactcc tgtacctatt ccatgtatta
     8341 ctgttacaga ctctaaacct gccacatatg catcatcaag atacttttct acttccatta
     8401 tagcttcttc taggtttaac cctctaaggt caacttcatt ttttacacta ccagattttg
     8461 accttataat gtttctcgtt gatttagtta catttgttga aacatctttg cgtgtttttt
     8521 gtaaagactt aaaaggtaga gtcatcttca ttataccaat ttgcactact gcttcttttc
     8581 tctttttatc tactgaaact acactaccat tttgatttaa agttattacc ttaacttctt
     8641 cacctggctt taagtctttt atctccttat ttgatacctt tggaactatc atacttttta
     8701 ctgttggctg aagacttccc atagagctgg tcaattcttt tctaagttct tctatcttac
     8761 gattcttttc ttttgaagct ctttcttgtt caagacttct aagttctttt attataatat
     8821 ctacttcttc tttagcttgt cttataattg aaaatgcttc tgatttagct ttttcaatca
     8881 tcttatctct ttgactgacc aatttttcaa gcttttcgtc atactctact ttcagttttt
     8941 caatctcttc ttttaatctt tcagcttctt ctctatcttc tactgctttg attctattct
     9001 tttcaacatt ctgtaacaca tcttctaaag ctatgttttc tgtatttata tattctttag
     9061 cacgtgaaat cacataatca cttagcccaa gttttctaga aatctcaaac gcatttgatt
     9121 ttccgggaac acctataagt aacttatatg ttgggcttaa agtttctata tcaaattcaa
     9181 ctgctgcatt ttctacacct ggtttagtaa gagcataatt tttaagctca ctatagtgag
     9241 tggtagctat acatttagct cctacagaat ttatatcttc taatactgct attgcaaggg
     9301 cagctccttc aacagggtct gttcccgctc ctaactcgtc aaatataact aaagagtctg
     9361 ctgttacgtt ttgtaatata gatactatat tcgtcatatg agatgaaaat gttgatagac
     9421 tctgttcaat actctgttca tctcctatat ctgcaaatac attatcatat acacacatac
     9481 tacttccata atcagcaggt atatgaagtc cactttgtgt cataagtgca aatagaccta
     9541 cagtttttat tgtaacagtc ttaccacctg tgtttggacc tgttatcacc aatgtatgga
     9601 agtctcttcc taaataaatt gtatttgcta ctaccttctt tttatccagc agtggatgtc
     9661 ttccattttt tatgtttaaa tatttatcct catttaaagt tggttctatt cctctcatct
     9721 gtatagaaag ttttcccttt gaaaatgcaa agtccaatct tccaagtatc tcttgattag
     9781 agattaaatc ttcgctcact tctccaacca tagcagaaag ctctgataaa attctttcta
     9841 tttcctcttg ctctcctagt ttcaactgcc tcaattcatt gttcatctct actatagtca
     9901 ttggctctat aaaaagtgtc gcaccagaag atgactggtc atgcactata ccagctactt
     9961 gcgacctata ttcagatttt acaggtacta caaatctatc acctcttaga gaaattattg
    10021 catcttgtaa gtacttttga taagtagttg aacttattat tgaatttaat tttgacctaa
    10081 tggattgatt tttttgggct attcttcttc tgatatctct aagtgtactt gaagcattgt
    10141 cagatatttc tacttcacta attatagcat tgtatatctg gtcttctatg tccttgtata
    10201 catacagaga atttgataga gattgtatta ttggataatt aaaatcttct tcatcactac
    10261 tagataagct atttcttaat cttctagcca ctcttaaggt atctgctatc ataatcagac
    10321 ttcctgggtc aagagaagca cctatataag ctctctttac cttatcttct atatcatgaa
    10381 taccttccaa ccctactgaa cctctcttta taataattga ttgagcttca ctagtttctt
    10441 ctagcataga tttcacttct acaaaatcag tatttggtac taaattttct atatatttta
    10501 gacctaaaga agatgaggct ttcttcttta acaaatctat tattttattg tattctagaa
    10561 ctcttaacga tttctcattc attttctata ttcaccatcc tcgatatttt aactttttta
    10621 tttaatttta aaaatgtatg ttttcaagca tttccaataa aaactgcaag ttttatttct
    10681 ttttcatttc taaaattatt atattttttc tcttttagat atattttaca catattttgt
    10741 attatactat gtatgtagct atctatatat tatacataaa catgctttat ttgtaatata
    10801 atctgttaaa aaatcttctt taatattttt aaaggagttt attttagctt ataaaaaaaa
    10861 atagctactt atatagataa attaaggagg aacatgagtc atgggaaacg tattagaagg
    10921 attaaaacca gaaagtgttt ttaaaaactt tgagaaaata tctcaaattc ctagaggttc
    10981 tggaaatgaa aaaggcataa gtgatttttt acttagcttt ggtaaaaatt taggtcttga
    11041 aactattcag gatgaaagct taaacataat aataagaaag cctgctacaa aaggatatga
    11101 aaattgtcca ggtgtagttc ttcaaggaca tatggatatg gtttgcgaaa aagaaaagaa
    11161 tgttgaacat gattttctta aagaccctat aaaattaaga attgatggtg acatgatata
    11221 tgctactgga actactctag gagctgacaa tggtatagct gttgctatgg gtatggctat
    11281 attagaagat aatactttag agcatcctgc actagaagtt ttagttactg ttaatgaaga
    11341 agatggtatg aatggtgcag atgcactaga ccctagctta attaaaggtc aatatatcct
    11401 aaatatggac tccgaggagg aaggatactt attagttagc tgtgctggtg gtaaaacttg
    11461 tgttgttagt ttacctgttg aatacaagga agttaaaggt gacaaacaag gattattagt
    11521 tgaggttact ggactattag gtggacactc tggtatggaa attgttttac aaagagcaaa
    11581 tgctaacaaa gcaatagcta gagtattaag tgtattgaat gttgattatg aattagcttc
    11641 tgttgatggt ggtactaagc acaatgctat acctcgtgaa gctaaatgtg taatagctgt
    11701 taataaagct gatgttgaat ctgctaaaaa acaaataaat gacattttaa ctgcatttaa
    11761 acatgagttt actacttctg accctggcat gacttactca gtagctgaaa cttctgtaga
    11821 caaagttctt actaaagatt gtaaagaaaa agttgttcaa atgtcttgct taactcctca
    11881 tggagttcaa tcagtaagtc ttgatataga aggattagtt gaaagttcta ctaacttcgc
    11941 tataattgaa actaaagaat ctactattga atttttaact tctgttagaa gttctgttat
    12001 gtctattaga gatgaaatcg ctgatagaat aagacttttg gctcaagctt taggtgctaa
    12061 ttacgacctt atagctcaat acccagcttg ggaatttaaa aaaggctcaa aattagaaaa
    12121 aatatgcagt gaaacttatg agaaattaac tggtaaagtt cctactgtta tggctctaca
    12181 tgctggtctt gagtgtggat tattacttga taaacttcct catgctgaag ctatctctat
    12241 aggaccagat atgtttgatg ttcatactcc aaatgaacat gttagtatcc cttctgtagc
    12301 taatgtttgg gattatgtta ttgaaatatt aaaatctatg aaccaatatt aaatttaagt
    12361 tttgattcta agtagatttt aattttaagt aatctatata aaattaactc tgtaaattaa
    12421 tactactgtt agtataatat ttactttcaa tattaactta tagaaataaa gcctaatgat
    12481 agataataac atgtatcatt aggctttatt gtaatttaaa tattattaag ctttattctg
    12541 ttttaaaatt aattcttata ctattttaaa tctatttctt ccttgtctct tagaatcata
    12601 aagagcatta tctgctattt gatacagttc atcatactct tgaccaggct ttgctataac
    12661 tacccccata ctaatagata aactttctcc atttggcaac ttaatgagat gaatatgatt
    12721 gtatacatca ttacatttat taataagttc ttccttattt attatatcat tgatataaac
    12781 actaaattca tctccaccca aacgacctac tatatcattt tttgcaaaac tttcatcaag
    12841 tacctttgca acatcaataa gtactttatc tcctaacaag tgaccataag tatcattaat
    12901 cgacttaaaa taatcaatat ctataatcaa taaagcacca tttttttcag ttgataaatt
    12961 caacaaattt tgtgcagtta attttctaat atatgctgca ttataaatat tggttaaact
    13021 atccctttga gccttatcta acaatctatt cttttcctct ttttcagaat caatattaca
    13081 tattttcccg attacataca ttggtatttt tctatcatta taaattattt ttgaagtaac
    13141 tctaatccaa tgaaaagtat catcaagcat aatagaatac atttcagttg ttccattttc
    13201 ttgtgatttt attacatcaa aaaacttttt attatattct ttccttattt tatctctttc
    13261 attttctata ttgccatttt cactttcctt atcattttta tccaaaccat aataagtttc
    13321 aatacccttt tctggcatag aaattatcaa tttatcttgc ttataatcat actcaaaaaa
    13381 gtattcactt gtcatttcat acaactgagt atgttttttg agttccaaag atatcttctc
    13441 gttcatactt gtacgagtat gtaaaccaaa tgccataaat ccaataatac aaattaataa
    13501 actggctaca ataaaaatag tctcaattgg attctgttgc ataatataaa gcaacgagaa
    13561 atcttgtcta tatgtagtat tttggtagac aattgattgc attttttctg atgaaatact
    13621 tataatagct ttattaaata tattcaataa ttcttgttta tcttttttaa ttataccaaa
    13681 acaaatactc tttgctttat aggtttgagg aactagacgc acatttttat acttaggttg
    13741 attcatatag tactgcatag aataaccttc tccataagtg taatctgcat agcctttatt
    13801 aacagcttct atacactctt ctaaattatc ataccaaact atattctctt caaactggcc
    13861 ataataatta acacctttgg ttagagctat acgttttcca tttagactat tctcatttaa
    13921 tttattatta atcatcatag tatattgagt agaaagaaaa ggccgagaca tagctatatt
    13981 atgttcacga gagtactcat aatcataaat cattcctgct acaatatcaa tttcatgttt
    14041 tttagccata tcttccaaat cttcttgaga ttttacaggc acaagttcaa attccaaacc
    14101 tgttttatca catatatgat ttaacaaatc aattgatata cctcttaatt ctcctgtatc
    14161 tttatctgca ttttgaaacg gtggtttgtt taaaagtact cctactttta atttttctgt
    14221 atctttaatg taattagttt cttcatcaga taaaaacatt tgattattct ctggcgtaaa
    14281 gtatttctca tataaagtag ttgaaaaata tgggtccttc tgttcaatat tattaattgc
    14341 tgaattaagc ttttgagtta agtctgtact gccttttggg gtaatgaaat aaaatggctt
    14401 tgatgagaat tttgctacag tacgtacacc ttctattggg ttcatactaa cattaagaag
    14461 tacatctgct ttaccactct ttagtacttc taattgctcg tcatttgttt tacaatatac
    14521 caattcaggt gacatcagat tcatcttaca atactcatct aactctttaa gtctagtatc
    14581 agaattttct attacagcaa tacggatttt tttcacatct tgtgaactca ttatgttatc
    14641 aatatctgta ttttcatata atgcttgtag tactgtattt gctactccat aattattccc
    14701 tgcataatta tatatttttt gcattggctc attatataac atccctccca ttatatcaat
    14761 ttcaccattt tctaacatct tcattaattt tgtaatagat tcatttatct ctccaggaac
    14821 ttgaacaaat tcataattcc atccagtata ctgtgcaatt tctagtaaat attcatatgt
    14881 atacccaaaa tagttacctt gctcatctat ttcagttaaa cctttttgaa taggataagc
    14941 aacacgaatg gtacgattat cattacctat agcgttgata ccaaaagaaa atatgcaaga
    15001 agtgaatata gatgtaatta caataattaa taaaagtaat ttttttaaat tcactacaca
    15061 atcctccata tagttaagat ttacagtata aattactgca taaacttgta gacatgaaaa
    15121 aatagattag tatataaaag gatatataaa tctacttaga gatttaaata tccttaatat
    15181 atacaatata tttaaattat aactcattta tatgtctatt acaacaaatt taccctgcta
    15241 aatacctact ctatatccca taattttaca agttgtagtg aagtttaagt tctatctcac
    15301 ctcaaattta gcagtgtttc tatttacagt tcttttatat ttcacatctg gataacccaa
    15361 tataaatgaa gtgataactg cttcgttctc gctcattcct aacagttctt ttatctttgg
    15421 ctctacattt gatgccataa caaaaaatcc attataacaa attcctaatc cttgagaata
    15481 ggccataagt tccatattag atgcagccag tcctccatct acataagcac ttccacctag
    15541 agacatatct cctacaacta ccataagtag tggggcatca taaaataaag aatcatttcc
    15601 gttctctttg tatcttttgt acattttctt aaatgtattt ttatacatag aacgtacagg
    15661 gtcattgtca tcagtatcta atgccaaatt ataaagacct tgtattgcca gttctttcac
    15721 ttcttttagc ttttctttta ctaatatgta tcttataggc tgtctattac cacctgttgg
    15781 tgtgtatcta ccagcttcca ctatgttttt tattttttct tcttctacct ctatatcttt
    15841 atattgtctt atacttcttc taaatttaat aaaattcaat aacttttcgg gttctaattc
    15901 aaaactttca cgattgtatt ctattacttc atttttatca aatccttgca tttctattgc
    15961 atctgttggg caaattgcca tacagtgacc acagttaata cagagaatat ttttaacctt
    16021 tgctttatca tccttaactt ttattgcatt tacaagacag tctgattcac acatcccaca
    16081 gcctacacat ttttctaaat ttatgtctat cattccaaat tattcctcct tttaaaaaat
    16141 ttactttagt ataaaacaaa tagttgtcta agtcaatttt tattttttta acaatatact
    16201 agccttaact acactttaat ggagaaaaat atacataaat tggaacttaa attagaactt
    16261 attcaaagta aaagataaaa gaagtagatg tctaaaagtg aactttttag ttaattagat
    16321 attctttatt gtatgaaatc aagtggatat ttatcatttc aacaataaaa aaggcttacc
    16381 tctattttgg atatgtccct gtcaagtaga catactaaaa aaaggctatc tttatgcact
    16441 gagatagccc cttttcccaa ctactattgt ttatcttaat attagtcaat aaatttaatt
    16501 tgctataacc tgtaagttac attttaattt gatgtaactt taaattacat ttttaagtag
    16561 tatctttaaa ttatattctt aaaatatatc attaaactac attttaatta atctaaaaac
    16621 tatatcttaa ttaatctaag aaacttacat tataatcttt tagtttccca ttatcatata
    16681 caatctttgc actaaagata tttccagtct tagcccaatt taagaacagc ctgtcacctt
    16741 cccacatatt taaatcgaat attttatcat tatcaaccca aattaaattt ccttctttac
    16801 aatcatttaa atctttttca gataattcac cttcaaactc atctgagaaa tacaaaaaca
    16861 tataccaatc ttcttcatct ttaaattttg gaaaagttat caacccttta tatgacaatg
    16921 atttaagagt aagaccagtc tcttctttta cttctctaat tacacattct tctggagtct
    16981 ctccttgctc aaactttcct ccaactccta catacttacc ttcatgtata tcatctttct
    17041 ttttatttct gtacaacatc aaagtcttgt catctttttc tatataacaa atagttgtaa
    17101 gtttcatgta tctcgccttg ctttctcttt atattctcaa ctaattattt tcagcacttt
    17161 caagcacatc acttatattc ttcattgcat taagagaaag ctctgcaaat tcatcaaatg
    17221 gaatccctat atcttctata cttctcattg catctctatt tacatttgaa gcaaatctcg
    17281 tttctttcat tcttttaata acagacttag tctttacact ggtaagtttt ttatctggat
    17341 aaactaaagc tatagcagtt ataaacccag ttatagggtc tgctgcatat attgcttttt
    17401 ccattaggct agttcttgta actcccattc catcatgatg accaagtatt gctctataca
    17461 tttcttcgtc accgaagttt tcttttttaa gcagttctac tgttgttgta gcatgtcctt
    17521 cacatatttc tcctggcttt cttccaacta tatctgaatc caaatcatgt aaaagacctg
    17581 ccactgccca ctcttcttct ttttctggct ctagtctttt ggctaattct ctcataacag
    17641 cttcaacagc ataacaatgt tttctcattc tatcagtttc taaatactta aataaaattt
    17701 ctaaagcttc ttctctcatt ttcaccaatc cccctgtttt ttcacatata tgatattttt
    17761 atatattaat acacataaat tcaagttttt atatatattc atgtatattt ttcatgttat
    17821 atctattcta cacctttata gtagtgtcct gcaactgttc caatttcatc taatttctta
    17881 tacaagtctg tagccttttt tccaagttta taattattac tcaatacctc aatgtgagct
    17941 tcaataacct ttttaaccat ctcactatct tcaactgtaa aatctatcct aaatacatct
    18001 attccaattt cctctaactc atatagattg tccaacatac aagtaacctt cgaattgtaa
    18061 attgtacttc tacagaatat atcttgtgtt atcctataac ttgcatccgt aaagtctctt
    18121 aaagcataac tactttcctt acatttagca actctcttgt cctttttaca atctctaaca
    18181 actactccca taggacagta ttctgtaacc atcataggag catatccata tattactgct
    18241 tcaatttcta catctgtata ttttaaagtt tgtttaattt catttaagtt tagttcttgt
    18301 gacaaacaaa ctgtacttgc tccctcttct ttgaagtggt ttatactttc actattgaat
    18361 gcatttaagt agcaatctat atttaagtct tttttcttaa agtattccat agagccccag
    18421 ttaccaattt gaaccttatt aattcctttt atattatcca atatctcaaa ttgcttgtac
    18481 tctttatttc taacaatcct tggtgctgaa tagattaatt ttttgtcagt gactgctgta
    18541 ttatagtggt tcttattcaa agtatctatt ttacttgtat ttgacaaagt atctatgtta
    18601 tttgtatttg aacccattag ttctttagga gtatctaaag acgaagaagt atctaaatcc
    18661 atagaaatac ttgaatctat agaaatattt gaatctatag aaatacttga gaccatttgt
    18721 aaagcttttg ataatgtatt tatatcttca taatagatta aatctacatt ataatttaat
    18781 actgcttcta attgctccaa gttcttaact ttaactctaa tcttaggaac tgtatcaagt
    18841 tttttctgtt tatttactct atttggctgg tattttaatc tattcttctt aaattttcta
    18901 tcttttatct ttattctttc ttcatctaaa agctcaatac aatcccttct catttgattt
    18961 aacacactta taggcatact tacaccttca tccaggtcaa tttctatctc ttttaactta
    19021 tatggtgtat ttccaagctt ttgaatttgt gtttctattt tttctttact taaagccacc
    19081 tttatagctt tttcaactat tttgtctcct tctaccaccg atttatttcc acaaaaatca
    19141 cttataaaaa gctctggctt tttacctagt ttaatactca cttttgcacg tatattagtt
    19201 tttatgaact ctttatcttg ctgaaatgta gcttgtactc tatccatcaa ttcgctgtca
    19261 gaagtcttat atataagctg atttctcttt gcttctccaa caaaatctaa ttctatcaaa
    19321 tcccctttat gtgctattgt agttatctct ccatccttta ttattcttcc aatagtacct
    19381 ccacctaagt taattccatc acctttctta agcgtatttt ctagcattac tctaagtctt
    19441 ttgttctttc tattataatc taaaactttt ccaatatata gcccttgatt attaggtcta
    19501 ttagagttca ttatatcctt accaacttca cctaatatat atccttttgt aaactttcta
    19561 ttaaatatgg tatacaaatt atttattatt tcctcagaaa tacttatttt tttcttatct
    19621 acaaattcat taatcgcttc tctatatcct gaaaccacag tagctacata ctctggcttt
    19681 ttcattctac cttcaatttt taaagataat acacctgctt caattatttt atccaaatct
    19741 tctatagtac ttaaatctct aggactcaac aaataatctc cattactttt tactacttct
    19801 cctgtattta tatctatcaa ctcatacttt tgtctacatg gctgtgcaca cctacctcta
    19861 tttccagacc tattgcctat catactactc ataaggcatt gacccgagta acatacacat
    19921 aaagccccat gtacaaaaac ttctatatct acagttgtat tttcacaaat ccattttatc
    19981 tcatcaacat ttaattctct tgctaaaaca actctatcaa accctacact ctgaaggtat
    20041 tttacatctt ctaaggaatg tgctaccatt tgagtgcttg catgtagttc aaagtcaggt
    20101 aatagttttt ttataagtct tgccattcct atatcctgta ctattaaagc atcaatatct
    20161 atatcgtata aaaattttac atactctaca aaatcttcta tttcatactg ttttatcaat
    20221 gtatttacag taacaaagac ttgcactccc cttatatgag agtatttaac agcctcaatc
    20281 agctcatttc tatcaaagtt atttgcagat gctctagcac taaagtcttt accacctaaa
    20341 tacacagcat ttgctccgtt ttgaactgct gcttttaatg aatcaaaaga gcctactggt
    20401 gcaagtaatt ctattttttt catatttttc tcctctattt atacttgtta ggttaatata
    20461 attatatcta taacttaaat atttgtatta aatttatata tttcttacta tattttaaat
    20521 tcttcaatca atttatatat tagttattta tatttggttc atttgagtta acatatacat
    20581 tatttactat atttagtata tttctattaa cttatatatt tattactgta ttttaaattc
    20641 ttcaattaat ttatatactg cttactaaat ttaaaacatt ttaattaatt tgtacactgt
    20701 ttactatact tgaaatactt ctattaatct atatattgcc tactatactt aagacatttt
    20761 aattaattta tacactgctt actatatttt aaattcttta attaatctat acaatatttg
    20821 ctatatataa agtatatcaa ataacagctt catttttttg taaatttaca gaaagttaat
    20881 tttcattaaa tgaatcatct ttattgataa acataattcc aaagaccact gccaaaataa
    20941 aaaaacatat tagtattgat ttttccacga aaatttttgt aaaaaaagtt cctaaaatag
    21001 ctccagatat aaagaatata ataatagcat aatactgtaa actctttttt atcataaaaa
    21061 tattcttagt atgtatataa tgaaataaag tttctgtagc acttcttaaa tttccagtac
    21121 acattgtcgt tgcaaaacta ttcccattta cttttctaaa actctctact tgcattgaac
    21181 aaataaaaga aatagcaaca tttacaaaca tattaaatct tcctctagga ataaatgaaa
    21241 caataagtat tgtaatcatt tcaattataa tgataacctg acgccaatga atctttgtct
    21301 tattatttgt aaaatacttt cttatcttct cggctataaa tacacctaac ataaatgcca
    21361 aaataggaat caaataataa aatgattcct ttacattacc ttctgctata ttaagcccaa
    21421 acaaaactat atttccagtt tgtgcattag caaagacttt acctcttacc acataagtat
    21481 aaatatccaa aaatcctcct gttattgcca acaaagaaca taaaaagaat gattcagaca
    21541 tctgactctt gcttgtcata tataaacctc tcgctttcta atgatagata caaaatcaaa
    21601 atcattttag attctatttt tcattgatat aaaaaaatta tttatattta aatattgtct
    21661 atatttatac atcatttata tttatttata tttaaacatt atctatatct atatagcatt
    21721 tatatttata cattaaaaga agctgtctca aaaaacaaga gacagcaaat cttttagttt
    21781 attccacatt ttttgcatca ttatttaacc ctgtacattt taactgtaaa tcataagctt
    21841 tgttttgaaa ctcagaagct aagttttcag ctatagtagc tctttcattt gcttcttcaa
    21901 gttgagtact taactcctca atcttgtttt tataagcatc taactctgct ctagaacctt
    21961 ctgtcgtatt actaagttcg aatatttctt gctttttatt ttctataatc tcaattaact
    22021 ctttcatctt agtttcagct tcacgatttt ctgcttcttt ttctgctatc gtaagcttta
    22081 aagatttgat ttctaattga agctcttcat tagatgctcc aaccttctta ctcaattctt
    22141 cattcgcttt aatcaacttc tcattctcat ctgaacattc aaataaaagg tctgatatat
    22201 ttaatgcagt taatatcgca gcaactgata agctcaattt cgggttttga cgtgttattt
    22261 tatccatttc tttgtctaca aaatcagcaa tactaatcat aaacttctct gatttgtctc
    22321 caaccattgg atattcagcg ccatggattt taaccattac tttgttcatc ctaacctccc
    22381 ccgacattag ttgttaattt gatcttaagt tagcatttaa tttttcgctt aattcactta
    22441 atatcttatc atgcacctta gctacatctt catctgttag agttttatct ttacttctat
    22501 aagttattga atatgcaatt gacttgtatc cagcttctat ttgagaacct ttgtaaacat
    22561 caaataactt gtagctttct actaatcctt gaccattttc ctttattata tcttctattt
    22621 gttttacaaa tacatcatct ttaacaataa gagctatatc tcttgatgta gatggatatt
    22681 ttggtaatgg cacataattc tttgttctat cagaattatc aaatacaaag tcaatatcta
    22741 tttcagcgac ataaactctt tgacctagat tgtagttttc tataacatct ggatgcaatt
    22801 cacctaatgt acctacatat ttattattat acactatttt agcacatcta ccagggtgga
    22861 aggtagtatt attatcttga ggttcaattt cataaccttt aaatccgaca tttacaagta
    22921 tagtttctat agttcctttt agtgcaaaga agtcaacatc tttaccatac atacctacac
    22981 acattctatt ttcttcctta ggtaattctg aatcttgtgg gatgaatata tgtccacact
    23041 caaatgcaga tacttcttct attttatgag atacattagt tgataaaaca tctaacatat
    23101 ttggtattaa agtagttctc attacagaag tttcttctcc taatgggtta agtaatttaa
    23161 caaaatttct ctttgcatta ccttctggta ctcttatctt atctactccc ttagggctta
    23221 caaatgaata tgttaatatt tcatttaatc ctacagcagt tgaattatct tttaaagcat
    23281 ccatgaattt ttgttttgaa gttttaattc ctgctgtcgt atttccttct aattgtacag
    23341 aaggtatatt ctcaaatcca taaattctag ctatttcttc ccatacatca gcttcttgtt
    23401 ccatatctgt tctaaagctt ggtacatcta tttctaattt atcattagct accaaattac
    23461 atttaaactc taatgattct aaaattccta caaactgctc cattggtaca tctacaccta
    23521 ataggtggtt aattctttga ggatttacta ccaatttttt aggttctggt ttatttggat
    23581 atacatctac aacacctttt aaaacttttc ctgctcctaa catttcaaca agttgtgcag
    23641 ctctatttgc tgctatctct gcaagattag ggtctaagtc tttttcattt cttgaagatg
    23701 cttctgacct aataccaact tttttagctg tcattcttat gttttctggt ttgaaattgg
    23761 cactttcaaa aagtacagtc ttcgtattag aagttatttc tgagttagca ccacccatta
    23821 caccagctaa accaagtgat ttttctccat ttgttataac tagcatatct ttgtctaatg
    23881 ttctctcaac atcatctaat gttacaagtt tctctccatc tttagcattt cttactacta
    23941 ttcttccagt ctctacttga tttatatcaa aagcatgaag tggttgacca agctctaaca
    24001 ttacgaagtt tgttatatcg actatgttac ttataggtct tactcctgct tctgtaagtc
    24061 ttctttgcat ccaatatgga gaaggttcta tttttacatc tgtaaccatt ctagcaacat
    24121 atcttctaca taaatctgga ttatctatct caactttgaa atctatctct tcgtcacttt
    24181 cttttacttc gatttcagga tattttactt ttgttcctat agttgcagct gcttctctag
    24241 ctatacccat catgcattta caatcaggtc tgtttgaagt taattcaaaa tctattaaag
    24301 catcttttaa tcctaaaaca tcttttatat cttttcctaa ttcataagaa tcttcgtggt
    24361 ctaaaatata tataccacct cttttatact cctcaatgta atgttcatct atacctagtt
    24421 cagctgctga acacatcata ccatctgata attcacctct gaaatcagtc tgtttgattt
    24481 caactcctcc aggtaactta gaaccattta cagctactgg aatataatct ccttctgata
    24541 tatttgtagc tcctgtaact atttgaacaa ctttatctcc aatatctact ttggttacaa
    24601 ccaacttatc agcatttgga tgttgtttta tttctaaaat ctttccaacc aatatatttt
    24661 ctatttcttc accataataa tctattgttt caactttagt tcctgtcatt gtcattttat
    24721 cagcgaactc ttttacatcc atgtctatat caacatagtc tctaagccat tttaaagata
    24781 ctaacatcta cataccctcc taattaaaat tgatttaaga atctcatatc attttcgaat
    24841 aataatctaa tatcatctat ttcgtattta agcattgcaa gtctttcaac cccaactcca
    24901 aatgcaaatc cactgtaaac ttctgggtct attccacaat ttctaagcac atttggatga
    24961 accatacctg aacctaatat ttctatccaa ccttcatatt tacacattgg gcaaccttta
    25021 ccaccacatt tgaaacaagt aacatcaacc tctgcacttg gttctgtaaa tgggaagttg
    25081 tgaggtctaa acttagtttt gatatcagaa ccaaacaatt tttcaacgaa gatatccata
    25141 gttcctttaa attctgccat agtaacatct tttccaacaa caagcccttc tatctgatgg
    25201 aacattggtg agtgtgtagc atctggcgag tctgacctaa aacatctacc tggtgcaatt
    25261 acttttattg gtaactcttg acttctcata gttcttactt gaactggaga tgtttgagtt
    25321 ctaagtaata ccccatcatt gatatagaat gtatcgctca tatctcttga tggatggtct
    25381 ttaggagcgt ttaatgcgtc aaagttgttt tcaacagtct caacttctgg cccttctgct
    25441 atagagaatc ccattccgat aaatatatct gttacttcat ctataatttg agttattgga
    25501 tgcttcttcc caacctttaa aacttttcct ggttgagtaa catctatcac ttcctctttt
    25561 aattttcttt ctttttcaat agcatttatt tcttcttttt tagaatttat gctaagttca
    25621 atgttttctc ttacctcatt ggcaacctta ccaactactg gtctttcttc agcagataat
    25681 ttacccattt ctttaagtat ggcagttatc tcaccttttt ttcctaagta cttaactctt
    25741 aaactttcta cactttctat gctttgtgct tcttttattt cagccaaagc tgcttcacgt
    25801 aaagcaagta atttttcttg cacacatatc accctttcgt ttttttgtat aaaaaaagcc
    25861 cctcaatccc tgtctaggga cgaaaggcta tagtttcgcg gtaccaccct agtagtttaa
    25921 caatttaaaa atcatcaaac cacctctaac aaacttaacg cgtttttgac gtcaaagcct
    25981 actgtaaaat ttcagctctg ttactccaga gggaacttct catgacctct tagaaaacac
    26041 tcccagccta ggtgtttatc tctttactaa gtttaatcaa aatactttcc tctttcattg
    26101 catttacaat attatattgt ttattttagt attatatcat ttccattcaa attatgtgac
    26161 tatttttaat atatatccaa tacataatta atttaattat tttttatata atatatatct
    26221 aatgcataat taactcaatt attttttat
//
LOCUS       DBGJFF010000053        25801 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.053, whole genome shotgun sequence.
ACCESSION   DBGJFF010000053 DBGJFF010000000
VERSION     DBGJFF010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25801
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.053"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(215..595)
                     /locus_tag="ACOIAF_002104"
     CDS             complement(215..595)
                     /locus_tag="ACOIAF_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HGZ2669114.1"
                     /translation="MCLKKLSKLELVIMKFIWNLDIKTNSYEIIDYMKEEHNLPEKVA
                     LKTLSKLSKKRFLYVQETGKCMYYTVAIKEKTYLEFISRNVQNLLKNNFIRNLLVSFH
                     EEELTEEKIKSLENWVVNWEEAYV"
     gene            complement(796..1989)
                     /locus_tag="ACOIAF_002105"
     CDS             complement(796..1989)
                     /locus_tag="ACOIAF_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HGZ2669115.1"
                     /translation="MLTSLALIFLLGMASGGIFKRIKLPNLLGMLLTGIILGPYVLNL
                     IDNSILDISSDLRKIALIIILTRAGLSLDINDLKKVGRPAVLMCFIPATFEIIGMIVL
                     APKLLGVSILEAAVMGAVVGAVSPAIIVPKMLKLMEEGYGTEKSIPQMLLAGTSIDDI
                     FVIVMFTVFTGLAQGNSISAISFLQIPVSIILGVIAGAVIGICLAVFFKKVHMRDSAK
                     GVLLLSISFLMISLETALEGIVPFSGLLAVMSIGIFLQIKYRVVARRLSIKYSKLWVG
                     AEILLFVLVGATVDISYAFKAGIGAVILIFGVLLFRMVGVFFCLIKTNLTIKERLFCM
                     IGYIPKATVQAAIGGVPLAMGMASGQLILTLAVLAILITAPLGAFGIDVTYKKLLTSV
                     KGESK"
     gene            complement(2052..3308)
                     /locus_tag="ACOIAF_002106"
     CDS             complement(2052..3308)
                     /locus_tag="ACOIAF_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HGZ2669116.1"
                     /translation="MIEKFKTLIWIKMIIRIKSVYKTYGGLFFLGLIGIPVGAIIGLI
                     DTIFGTVLLKVTDIRETYPMYLIPFLAVVGVVIAYCYFKFGGKSSKGMNLIFEVGHGE
                     EEIIPLRLVPFIISGTWLTHLFGGSAGREGVAVQIGATFSHWVGKRLPIKNASSIFLV
                     TGMAAGFAGLFETPIAAILFAMEVLVAGSLEYQSLFPAFTASFTASAVSKALGLEKFS
                     FALSSKVVFDLSIFWKLIVLGIIFGMVGGAFAWCLKLSKRKIGNRLKNPMIRIAIIGV
                     CLSVLFLLFYKGRYSGLGTNLIQNSFYGGEIYSFDWLLKFILTILTLSAGFQGGEVTP
                     LFSIGASLGVLLAGFFNLPIELVAALGYASVFGSATNTFFAPVFIGAEVFGYSYLPYF
                     FVVCAISYIFNMDKSIYSLQKISTKQ"
     regulatory      complement(3573..3649)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(4647..5381)
                     /locus_tag="ACOIAF_002107"
     CDS             complement(4647..5381)
                     /locus_tag="ACOIAF_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HGZ2669117.1"
                     /translation="MNLAKGLDVLKISSNVFGEDKVMYIPVIYTEDDATLIDTGLPGQ
                     GDLIIDALNKSNTSFDRLKNIIITHHDIDHIGNINYLREKSKNNIKVYAYKSEVSYIT
                     GEETPFKLYMLEQMVDKIDDKMLSMLNVMGLGFKSSYTKVDVSLDNHEKLNLGEEIEV
                     IHTGGHTRGHICLYLKESKVLIAGDLLQVENGELKPVDVMYSNKQELKDAIKNISNYN
                     IETIVFSHGGLYQRNIIGTLKNLIIE"
     gene            complement(6387..7025)
                     /locus_tag="ACOIAF_002108"
     CDS             complement(6387..7025)
                     /locus_tag="ACOIAF_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HGZ2669118.1"
                     /translation="MLIHLVKKDILIVKKYVLLILFIAIGIPLFILWRLPPFAQILGF
                     VLSIIFSEFMLCQYLLLKESQYSKASVLLCSTPYPRRGLVQSKYVLFAMVFIYCTLVY
                     WIENLLIPQIGTFDLTYILIVLLISSIIYGVYMPIQYKLGYETTKFFFVVIIMASSFV
                     LPLTIAHSNFEIPFIYQYPSMIVNIVLFLLSLIILLASMFISIKIFSNKELV"
     gene            complement(7028..7885)
                     /locus_tag="ACOIAF_002109"
     CDS             complement(7028..7885)
                     /locus_tag="ACOIAF_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2669119.1"
                     /translation="MENILEVYNLNKTYTDFSLSNISFALPEDCITGFIGVNGAGKTT
                     TIRSILDVVKRDSGKIKIFGKDFDGNEKELKNRIGVVFDDSYFYEELSMAEMKSIIAP
                     SYDNWCEEDYQQNMKRFNLNPKQKIDTLSKGMKMKFALALALSHKAEFLIMDEPTSGL
                     DPLIRSQILDILTGYMKKCGRGVFFSTHITSDLDKIADMIVMIDNGRIILEREKDTLL
                     DEHRKVKGDIQFLNDETKKLFLNLEITGYGFIGITSKINEVQKNMPDILIERPTIEDI
                     MLAYIERRE"
     gene            complement(7887..8261)
                     /locus_tag="ACOIAF_002110"
     CDS             complement(7887..8261)
                     /locus_tag="ACOIAF_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGZ2669120.1"
                     /translation="MKVLISNTSDKPLYQQIKEQIKEAIFTEELKEGDAMPSIRNFAN
                     DLKVSILTIRRVYEELENEGFLTRQVGIGTFISTGNLEILRDSKRRIVEQKMADMIKD
                     AKALGITQIELQEMMDILYQED"
     gene            complement(9440..10630)
                     /locus_tag="ACOIAF_002111"
     CDS             complement(9440..10630)
                     /locus_tag="ACOIAF_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HGZ2669121.1"
                     /translation="MSDMLLWFSVVLVLFAIGDLIASKTKAKVSAVFVTLLLFLILFV
                     TKAIPADIIEKAGMTAAASWSVPMIMFSMGTMLNVKQFIDEWRTVLTAWLGIVAVIVC
                     VSLCIPLFGKSTVLTSIPVINGALPATTIMTQAALEKGLTLAAATATVVFAIQKFIGT
                     PIASRAALQEANRLLVEYHEAKSKGIDLANVDTDKKEAENTGTKVKQAFCEKYDKYYS
                     TNVCIFFIALFSYLGYELSEIIHVNYSIVCLVVGVIVTRIGIVPKDILEKGKIKGFIN
                     MVVFAAVIPSLAKVSLTDLISLFVPIVGMFAASIIGIFLMMKVLPGWKIIGSKPLAFG
                     VGFCQMLGFPTTYLISNEVCNAVGETEEERAYLMSKIMPKLVVGGMACMISIVVAGLM
                     VPML"
     gene            complement(10646..12601)
                     /locus_tag="ACOIAF_002112"
     CDS             complement(10646..12601)
                     /locus_tag="ACOIAF_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HGZ2669122.1"
                     /translation="MEKIKIESSDIFEYIFPHDISCSPDGKHIAYIISNINEEKDCYE
                     HDLYVMDIKTEKQIHMTQTKDVTSFSWISNTELLFTSKRNKPKASTTDFYTISIEGGE
                     AKKAFSIPKACSVPVSLGNKLWLLTTKNPTDSKKSEPDRAVEGVDYWTFTDKPFIRDG
                     ENFSQRRRVTLELYQEGENITKAITPKFCEVAGVDVSSDKNRILYTGQIYEDCATPFS
                     GLWEYHIDSGETKELVPQGKYQISLAKYIGKDKVMLQASTLDRSITQNHDIFILDLST
                     SEINMIASPDGMYATLLDVDAVYGGGRSNKVIGDKFIGARICRTMTEFNEFDTKTGNI
                     RIITKVDAFTSFDIYDNTMYTVMLKDYELAEIYSIDMTTGTMKKMTAFSEPYLDTHKV
                     SLPEKLTFVAKNKEEVDGFVIPPIDAKEGEKYPAVLFIHGGPKWAYGYMFTHLKQCVT
                     SKGMYVIYCNPHGGDGYGEKFLEMVERWGYVDYEHLMEFVDTCIEKYPGIDADRLGVA
                     GGSYGGYMTNWIIGHTDRFKAAVSQRGISNLITASLIIDFGDRIMKQTCGDKTPWNHE
                     EVLWNHSPIKYVKNVKTPTLFLHSDRDYRCFMGDTFQMFTALKQLGVDTEMYLFHGDT
                     HGLSRNGRPSNRIARANAIVDWFERYL"
     gene            complement(12618..14663)
                     /locus_tag="ACOIAF_002113"
     CDS             complement(12618..14663)
                     /locus_tag="ACOIAF_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HGZ2669123.1"
                     /translation="MKKITKERIENTMKKLFTSETFLEYQFISDCQISPNGGYTAFIV
                     KKADIKENGYTSQVYILNNKTGELKQITSINSVGAYAWEDENTILFPALRNEKVKDAV
                     KNGKQCMSYYALSLNGGEAEELFRLPIKGGKLNPIGKGLYAVVDSYDNDRPIVEGLPE
                     EEQQKKINAYNKRHYEHFKEIPYAVNGEGYISRKRKRLYVYDSNINELKAITAPMFNV
                     VGMKISDGKILYIGQEFKDVKGLKNGVYVFDTNTNTNVCILEKDKYIIKGFELYQNQV
                     ILNLTDALSYGNGENGDFYTIDIDTKEMKLLSGHQHHCIGNTVTSDVKMGAGQTTKVD
                     GEYIYYTSTVDMDCIIERIHIPTGKQEKVTQTGSVDFIDVKDGNIVCVGCLGNGLPEV
                     YTVENGTLCKKTHLNDHILEEYKISVPEYIESKGSSKWEIQGYVYKPVDYEVGKKYPA
                     VLAIHGGPRLTYGPYFMNEIQVFTSAGYFVFFCNPRGSEGRGNAFADIRKQFGDIDYI
                     DFMEFTDTVLEKYTDIDKTKLAVEGGSYGGFMTNWIIGHTNRFAVACAQRSIANWSGM
                     EGTTDIGYYFCKGQTGASHMENHELQWKQSPLAYADKCVTPTLFLHGEKDYRCYMQEA
                     FQMFSALKIHGCPTKLCLFEGENHELSRSGRPKQKLQRLVEMLDWFSVYIKKDNIGQ"
     gene            complement(15000..15650)
                     /locus_tag="ACOIAF_002114"
     CDS             complement(15000..15650)
                     /locus_tag="ACOIAF_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGZ2669124.1"
                     /translation="MKVKSNLDEILYHKIIESLIRGEYSVGQKILLNDLCEKFEVSRT
                     PVVQAVKMLNKDGVLTIMTNGKVYVPEYEYDMVKQVCETRTLIETYALEKMMQEEEEV
                     FQQKLDVIKRYSDKCEEYYRQEKSVELALADLELHKAIVEGANNEILNDVYVGIQGRF
                     IVVNYLIRPLKNRNYEGTVQDHFEILKFIEDRDTKKAVDKLRNHIQGTIKRFCEDE"
     gene            complement(16634..16807)
                     /locus_tag="ACOIAF_002115"
     CDS             complement(16634..16807)
                     /locus_tag="ACOIAF_002115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669125.1"
                     /translation="MLKQMLENNLPIKIVSWDSEELYRNSLNSCSLGNYIPLIEYLSS
                     LEDFREVYKMLWK"
     gene            complement(16811..16966)
                     /locus_tag="ACOIAF_002116"
                     /pseudo
     CDS             complement(16811..16966)
                     /locus_tag="ACOIAF_002116"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
     gene            complement(17067..17747)
                     /locus_tag="ACOIAF_002117"
     CDS             complement(17067..17747)
                     /locus_tag="ACOIAF_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2669126.1"
                     /translation="MIHLMKLELKKVGLKRYFLFSILGIFLCMFFVFTGLNDKSTSIY
                     DYDVTFRTISLVFCFYYIILFAVLNVAYIINEYTNKTILILFSYPLDRKKLILSKLLL
                     ITTLMISSMIVGYICCFTFVVVLDKYFNLVIGDFHISILSYWIPGAIKSIIVFCSLGI
                     WTFVVGMIRKSIPVTLVSSIIFINLRQFILAGTNTTQDSLISVFIIVGITVIGIYYIL
                     NHKVTEID"
     gene            complement(17744..18670)
                     /locus_tag="ACOIAF_002118"
     CDS             complement(17744..18670)
                     /locus_tag="ACOIAF_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2669127.1"
                     /translation="MNYIIETHQLKKIYKDKAVVNAVNIHVKKGEIYGFVGPNGAGKS
                     TVMKMLLNLVKPNSGEIVMFSKKVAETDFEILKKIGTIIENPYFYENLTAKQNLDLHC
                     EYMGYYNKEHISEVLECVGLSKQSNKKVSKYSLGMKQRLAIARAILTKPELLILDEPI
                     NALDPEGIREMRELFRKLNTDYGITIFISSHILSEVEQIADTIGIIQDGKLIKEISMS
                     DIHKYQTDYVEVDVDNVELAGYLLEKEFGIKDFKITSESCIEIYDLRKDVKEISKIFI
                     QNQIGINSIGRKQSSLEDYFFQTTGTGGKETL"
     gene            complement(18743..19660)
                     /locus_tag="ACOIAF_002119"
     CDS             complement(18743..19660)
                     /locus_tag="ACOIAF_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGZ2669128.1"
                     /translation="MELFLVVISIVLFAFYLKERNKSKKMESDYKYINSRLRDITNDD
                     EINYILVPSDISIVKETAVDINKLLEKFYSRQINYNRSEKAILQIFTNISHDLRTPIT
                     VLKGYIEMLYLQSQKEELSSSMRATVEKMQTNSNELVNSINNLFNMAKIQSGDMILNL
                     QKSNLTQLCHEIILEFYDLLDKENFNVEVNIEDRPIYANVDTEALQRILKNLIDNAIK
                     YGSDGRFLGISLYEKEKHVFIDIEDKGSGISETEKEHIFTRAYTADRKKGNGLGLAIS
                     QGLANSMGGSIYVNSRPNVKTVFTVKLKS"
     gene            complement(19660..20349)
                     /locus_tag="ACOIAF_002120"
     CDS             complement(19660..20349)
                     /locus_tag="ACOIAF_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGZ2669129.1"
                     /translation="MDILLVEDDKNIREMVYDFLISENYSVKTAIDGNTAIEIFKNYS
                     FDIILLDLMLPKSSGFEVLKYIRSVSVVPVIITTAKDSDTDKMLGLNLGADDYITKPF
                     SIVELLARIKANIRRATVYSSSRNSQDMVIQYKDLIINISKYVVSKNGIDLNLTHTEF
                     EIVKLLATNQGRAFSKEQLYNAIWKEPYYGNENVLNTHINRLRNKLSDGMEKNNYIKT
                     LWGIGYKMEEE"
     gene            complement(20757..21062)
                     /locus_tag="ACOIAF_002121"
     CDS             complement(20757..21062)
                     /locus_tag="ACOIAF_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669130.1"
                     /translation="MTSMIMEVVGVLFIAIGLILTFIEIKGNFPSFYGFGQNSIGKNA
                     VGIIAAGIALIFFRNGIDSIDSIATYGVFLAIELVVIVAYFLIRKKFTKEDKHLRTN"
     gene            complement(21786..22004)
                     /locus_tag="ACOIAF_002122"
     CDS             complement(21786..22004)
                     /locus_tag="ACOIAF_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGZ2669131.1"
                     /translation="MSVLLKTKIKSYREKNLMTQGELAKSVGVRRETIVHLENGRYNP
                     SLKLAMDIAKVFDTTVEELFEFIEENNI"
     gene            complement(22005..22412)
                     /locus_tag="ACOIAF_002123"
     CDS             complement(22005..22412)
                     /locus_tag="ACOIAF_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669132.1"
                     /translation="MSKQQTGIKSTLISEILGISYCIGATLVNKYFFKGSSIEYLPII
                     IWVLAMGAQWVYNKKYDDLIDEASKCILSRVNDIAIKVLFFSVAIVSIFLVTLDINVS
                     NLNIGMSLLVILFIQSLLKLILFIYFDRKGIYN"
     gene            complement(22498..22986)
                     /locus_tag="ACOIAF_002124"
     CDS             complement(22498..22986)
                     /locus_tag="ACOIAF_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3841 domain-containing protein"
                     /protein_id="HGZ2669133.1"
                     /translation="MKFYTIQTLEFWNNNKNNKYLSNDYSFILEDFDIPYKWMYSQMT
                     NRIYEFDDSMIWVWTKRPDLRCSGYLEKGKKGVLLEIEISEDNVLLSEFDLWHLPLMD
                     ITVELYEDEQIEKKVSWERIFDLELCSKLNEVNIEDLTLQGVTSKVPLSNVKLIKEFV
                     AK"
     gene            complement(23191..24288)
                     /locus_tag="ACOIAF_002125"
     CDS             complement(23191..24288)
                     /locus_tag="ACOIAF_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HGZ2669134.1"
                     /translation="MKEKLEEKSKIIKTSKEYKRTIIEEKDREWTKKLNDILSRGDFS
                     NEKLDKIKDLIPKEILTSENVGEVVTEDGYAKPEYDEVFKSLFTLNNDYDLLANFIND
                     ILKDAPYANRNIKQFTQIKRIIKVETDPTINYIGEKRPRLDILAEDEERNHINIEMQR
                     ALEEDYLERAEYYLSRVHGRKLEEGKEYKEIGKTVGIHILNHVKYNHIEDYVNCLRLT
                     MDGHPDIFSSKTALYFIELPKIRKSSCIANRVLIWGKLIDNPSHIDIRILSKTDYVIK
                     RALDRLKELGSNEDYLLNLKRGAYIMNKSRNFKEEIFQEGIEKGIAKGKREEKFNIII
                     KLKNKGYNLSEICDIIDDLNKSEVEKVYNQN"
     gene            complement(24662..24832)
                     /locus_tag="ACOIAF_002126"
     CDS             complement(24662..24832)
                     /locus_tag="ACOIAF_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669135.1"
                     /translation="MDSNNNTIKSTVKKGISFGSCLAMIISYTAWKSIPWAIFHGLMS
                     WIYVLYYWVKYA"
     gene            complement(25004..25447)
                     /locus_tag="ACOIAF_002127"
     CDS             complement(25004..25447)
                     /locus_tag="ACOIAF_002127"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGZ2669136.1"
                     /translation="MIEIKTIEINKKSQIDLLMLADPCEEMIDKYLNKGTMYALYDNK
                     ELTCIAVVNEISKEICELKNIATYEHFQNMGYASKMIYHLLDVYSKKYSSMVVGTSES
                     GVPFYEKFGFVYSHKIKNFFVDNYPEPIFEGELQCVDMLYLSYNF"
BASE COUNT         8891 a         4367 c         2709 g         9834 t
ORIGIN      
        1 acaagttcta gattactata gagatgacat atcaaaatat ttttaaaaag taatttaagt
       61 attagaagta attgaagtta gtaaatataa atgcgtttac ttttaagtat tgcttaaggg
      121 aataaaaaat aaaaataaac tctgcaacca tctaaaaata gttgcagagc ttacaaaacc
      181 aaaattatct aaattgtaat aaaaataata acatttatac ataagcttct tcccaattta
      241 ctacccagtt ttctaacgac tttatctttt cctctgttag ctcctcttcg tgaaatgata
      301 ctaataaatt ccttataaaa ttattcttca aaagattttg cacatttctt gatataaact
      361 ctaaatatgt cttttctttt attgcaactg tataatacat acacttgcct gtttcttgaa
      421 catataagaa tcttttcttt gatagttttg ataaagtttt taaagctact ttttctggta
      481 aattatgttc ttctttcata taatcaataa tctcatatga attggttttt atatctaaat
      541 tccaaataaa tttcataata actaactcta attttgataa tttttttaaa cacatagtta
      601 tcactccgct tctttatcta tataattaaa tattacaata ataatttcac aaaatcaatt
      661 ctaataatca taacatacct taaaatctgt tcttaacttc taccttctta atctcccaaa
      721 tgattttttt atattgtatt caatagtata atccttttcc aaaatataac ttaccagtta
      781 aaataatctt tatttttact tactctcgcc ttttacagaa gttaataatt ttttataagt
      841 tacatcaatt ccaaaagctc caagaggtgc agttataagt atagcgagaa ctgctaacgt
      901 caaaataagt tgtccagatg ccatccccat tgcaagtggt acacctccta ttgctgcctg
      961 tactgtagcc tttggaatat agccaatcat acagaacaac ctttccttta ttgtcaaatt
     1021 tgtcttaata aggcagaaga atactcctac catacggaat aaaagaactc caaatattaa
     1081 aattacagct ccaattcctg ctttaaatgc ataacttata tctactgttg cccctaccaa
     1141 aacaaacaag agaatttcag caccaaccca tagtttcgaa tactttatag acaatcttct
     1201 tgcaactaca cgatatttta tctgaaggaa aatcccaata ctcataactg ctaataatcc
     1261 agaaaatggc acaataccct ctaaagcagt ttctaaactt atcattaaaa atgagatact
     1321 taaaagcaac acccctttag cagaatctct catatgaacc ttcttaaaaa atactgctaa
     1381 acagattcca atcactgctc cagctattac acctaatata attgatactg gaatttgtaa
     1441 aaaactaata gctgatatac tatttccttg tgccaaccct gtgaatactg taaacatcac
     1501 aattacgaaa atatcgtcta tagaagttcc tgctaaaagc atctgtggaa tacttttttc
     1561 tgtaccataa ccttcttcca ttaatttaag cattttaggt acaataattg cgggagaaac
     1621 tgctcctact actgcaccca tgactgctgc ttccaatata gaaacgccta gtaattttgg
     1681 tgctaaaact atcattccaa taatttcaaa agtagctgga ataaagcaca taagtacagc
     1741 aggtcttccc actttcttca aatcgtttat atctaaagat aatccagccc ttgttaatat
     1801 aataatcaat gcaatctttc taaggtcaga agaaatatct agtattgaat tatcaatcaa
     1861 atttaaaaca tatggaccta atataattcc tgtaagtaac attcccaata agttaggtaa
     1921 ttttatcctc ttaaaaatac ctcccgaggc cattcctaat aaaaaaatca aagctaaact
     1981 tgtcaacata ttcctaccct atcctttctc taatattctt tctcttctaa tcatacatta
     2041 tgttgtttta tttattgttt tgttgaaatt ttttgtagtg aataaataga tttatccata
     2101 ttaaatatat atgaaatagc gcatactaca aagaaataag gtaaatagct gtatccaaaa
     2161 acttctgccc ctataaatac aggtgcaaaa aaagtatttg tagcactacc aaagacacta
     2221 gcatagccaa gcgctgcaac taattcaatt ggcaaattaa aaaatcctgc taacaataca
     2281 ccaagactag ctccaatgga aaacaaaggt gtaacctctc ctccttgaaa acctgctgac
     2341 aaagtcagta ttgtaagtat aaactttaac agccagtcaa atgaataaat ttctccacca
     2401 tagaaactat tttgaattaa atttgtccct aaacctgaat atcttccttt ataaaataaa
     2461 aggaataata cactaagaca tactccaata atagcaatcc ttatcatagg attttttaat
     2521 ctattaccta tttttctttt tgacaatttc agacaccatg caaatgctcc accaaccatt
     2581 ccaaaaataa ttcctaaaac aataagcttc caaaatattg acaagtcaaa tacaacttta
     2641 gaagaaagtg caaaagaaaa tttctctaac cctaaagctt ttgatactgc acttgctgta
     2701 aaagaagcag taaacgcagg aaaaagcgat tgatactcca gtgaaccagc taccaaaacc
     2761 tccatagcaa atagtattgc tgctattggt gtctcaaata atcctgcaaa accagctgcc
     2821 ataccagtta ctagaaaaat actagaagca ttttttatag gcaacctttt acctacccaa
     2881 tgtgaaaaag ttgcaccaat ctgaacagca actccttctc gtcctgcact accaccaaat
     2941 aaatgggtta accatgtacc tgatattata aatggaacca atcttaaagg aataatttct
     3001 tcttctccat gccctacctc aaatattaga ttcattcctt tactactttt tcctccaaat
     3061 ttaaaatagc aataagcaat aacaactcct actactgcta aaaatggtat cagatacatt
     3121 ggatatgttt ctcgaatatc tgtaactttt agaagaaccg ttccaaatat ggtgtctata
     3181 agacctataa ttgcaccaac tggtatacct atgagtccta aaaaaaatag tccaccatat
     3241 gttttataaa cgcttttaat tcttattatc attttaatcc atattaaagt tttaaatttt
     3301 tctatcattt taattccttt ctatttgcat cataagattt tttgaaagtt ttattacatc
     3361 aactgtagtc aatgtaatga ctatcataat tgttttttaa attgatagga tataggtaca
     3421 ctcttaaccc attcttaata aaataattaa catataatct actatttttt ctattgatta
     3481 aatatttctt caaatttaaa aaataaaaat ttagtaagta attcacctcc attttatcga
     3541 tttaaacaac aaaaaaagct ggtggcatct atgatatcat agaagtcatc agctcattgg
     3601 cagtttagga tattatcatc caagggagaa cctcattccc ataattatta tataattttt
     3661 tatttttcat atgtctattt tatgtaaatt gtatttttaa tgttataaat aatataacct
     3721 atcaattaaa atattaaagt ctctaatatt atgagtgttc aatatttcat attattttta
     3781 tgatatataa tttaaatgat tttctttact ttgtattatg atacataact taaatatttt
     3841 tcctcactcc acattatgat atattattta aatatttttc ttcactctac ttatattatg
     3901 atacatgatt taaatatttt cttcactctg cttatattat gatacatgat ttaaatattt
     3961 ttactcactc tacattatga tatattattt aaattttttt cttcactctg cttatattat
     4021 gatacataat ttaaacattt ttcttcactt tgcattatga tatatggttt aaataatctt
     4081 ttttactttg tattatgata catgatttaa atatttttac gcactttata ttataataca
     4141 tgctttaaat aatcttattc gctttgtatt ataacacatt atttaaatat ttttactcac
     4201 tctgcattat ggtacattat ttaaatattt ttcttcgctt tgcattatga tacatgattt
     4261 aaatattttt actcactttg tattatggta cattatctaa atatttttct tcactctgta
     4321 ttatgatata taacttaaat attttacctc actctacaat attatatata atttaaatat
     4381 ttttcttcac tttgtatact aaagtttatt taaagtattt tctttcccat tatactatgc
     4441 tgttaaatat ttttcaactg aagtattgta gaaccttaaa attactataa aaaactatat
     4501 tctaaaatac ttgtaatact acatatcacc cttaacactt aagtataatt ttaaagcaaa
     4561 attcttatta cttatattta attttgattc tacatcttta gatataattt taagtaattt
     4621 aagaaattat tgtaaatcat atagtactac tcaataatta ggtttttcaa tgttccaatt
     4681 atgtttcttt gatataatcc accatgacta aaaactatcg tttctatatt ataattactt
     4741 atatttttaa tagcatcttt taactcttgt ttgttgctgt acatcacatc aacaggctta
     4801 agttctccat tttctacctg taacaaatct ccagcaataa gtactttaga ctcttttaaa
     4861 taaagacata tgtgacctct agtgtgacct cctgtatgta taacttctat ttcctcacct
     4921 agattcaatt tttcatggtt atctaatgac acatctactt tagtatatga tgatttaaat
     4981 ccaagtccca taacatttag catactaagc atcttatcat caattttatc taccatttgt
     5041 tccaacatat aaagtttaaa aggagtttct tctcctgtta tatagctgac ttcacttttg
     5101 tatgcatata ctttgatatt attcttagat ttttctctta gataatttat gtttcctata
     5161 tggtctatat catgatgagt aattattata ttttttaacc tatcaaagct cgtgttactt
     5221 ttattaagag catcaattat taaatcacct tgacccggaa gacctgtgtc aataagtgtt
     5281 gcatcatctt ctgtgtagat tacaggaata tacataactt tatcttcacc gaatacattt
     5341 gatgaaattt ttaaaacatc taatcccttt gctaaattca taatttttct ccttataatt
     5401 acttaattag aggtcaattt aattaaagaa attgagaaaa tgtagatttt cctctagtta
     5461 tattttatta attaaaaatt attaatacct agtatatcac ttaagttgaa ggattactcg
     5521 acttgatttt tttgttattt tatggtatag taattaatat agaagtaagt aattgcaagc
     5581 tttgcaatta cttttatttt tttctaataa ctattttatt tttgataatt cgtataaaat
     5641 taaaagtaaa gtaatatgga aaatatgaat atagaaaaac tttaataaga aaataaatta
     5701 tgaataaata aaagaaacgc aatactgtat atagatgatt atttagaaaa ataaaatctc
     5761 ttttaataaa aataggcttc aaaataaact tttagttcat aaagcttagg agtaactaaa
     5821 ttgagtgcaa gtgtatctac tgttcaaaat aaatagttca taaagcgctc ttttttatat
     5881 taaaccaatt atattttcgt catttcagca atgaaaaaga agcttatctc tattttgaga
     5941 cagcttcttt ttattaactt cctacttttt attagttttc taaaattgaa aagctttatt
     6001 tataggattc cttagattta tattttgacg atataatttt ttaaaaacat cttaaaaaat
     6061 ggtatacaat acacacaatc atactcataa ttgaaaaata atcaatggaa aataaaatta
     6121 ctggatatta tctattaaaa aatacaaatt gattcacaag aaacatataa tctataatat
     6181 tatgatgtta ctctgccaca ccaagtaact ttattttcaa gtaacctttt cataccatct
     6241 aactttactc aataaaaatg gcaatataaa tatgtactct attttttact tttaaatatc
     6301 tgacaagctt tatatactat tggaagtaag atacaaagta aaacagccca atctggcaaa
     6361 tgtggcatat aaaagtcccc cttaccttat acaagctcct tattactaaa aattttaata
     6421 gaaataaaca ttgaagctaa cagtataatt aaacttaaaa ggaacaatac aatattcact
     6481 atcatggatg gatactgata tataaatgga atttcaaaat tgctgtgagc tatagttaat
     6541 ggtaatacaa atgatgaagc catgattatc accacgaaaa agaattttgt tgtttcatat
     6601 cctaatttat attgaatagg catataaact ccataaataa tagaacttat taataaaaca
     6661 atcaaaatat atgtcaagtc aaaagttcct atttgtggaa taagtaaatt ttcaatccag
     6721 taaactaatg tacaatagat aaataccatt gcaaataaaa catacttaga ttgaactaag
     6781 ccacgtctag gataaggagt tgaacaaagt aatacagatg cttttgaata ttgactctcc
     6841 tttaataata gatactgaca cagcataaat tctgaaaata taattgatag tacaaatccc
     6901 aatatttgtg caaatggagg taaacgccat aatataaata atgggattcc tattgcaata
     6961 aataatatca acaatacata tttttttaca atcagaatat cttttttaac taaatgaatc
     7021 aacataatta ctctctcctt tcaatatatg caagcataat atcttcaatt gtaggacgct
     7081 ctattaaaat atctggcata tttttttgca cttcattaat ttttgaggta atccctataa
     7141 aaccataacc tgtaatttct aaatttaaaa ataatttttt ggtttcatca tttagaaatt
     7201 gaatatcccc ttttactttt ctatgttcgt ccaatagagt atctttttca cgttctaata
     7261 taatacgtcc attgtcaatc ataacaatca tatctgctat tttgtctaaa tcagatgtta
     7321 tatgagtgga aaagaaaaca ccacgaccac atttcttcat atatcctgtt agtatgtcaa
     7381 gtatctgact acggattagt gggtctaaac cacttgtagg ctcatccata attaaaaact
     7441 cagctttatg cgataatgct aatgctaaag caaatttcat tttcattcct tttgaaagtg
     7501 tatcaatctt ttgttttggg ttaagattaa aacgcttcat attttgttga taatcttctt
     7561 cacaccaatt atcatatgaa ggtgcaataa tacttttcat ttctgccatt gacaactcct
     7621 catagaaata tgaatcatca aatacaactc caattcgatt ttttaattcc ttctcatttc
     7681 catcgaaatc tttaccaaag atttttattt ttccactatc acgctttacg acatccaata
     7741 tcgaacggat tgttgttgtt tttcctgctc cattaacacc tataaatcca gttatacaat
     7801 cttctggtaa tgcaaagctt atattagata atgaaaaatc ggtataagtc ttatttaagt
     7861 tataaacttc taaaatattt tccatattaa tcctcctgat atagaatatc catcatttct
     7921 tgtaactcta tctgtgtaat tcctagtgct tttgcatcct taatcatatc tgccattttt
     7981 tgttcaacta tacgtctctt tgagtctctt agtatttcta aatttccagt tgaaataaat
     8041 gttcctattc caacttggcg tgtaagaaag ccttcatttt caagttcttc atacactcgt
     8101 ctaatagtca aaatactaac ttttaaatca ttagcaaaat ttcttatgga tggcattgca
     8161 tccccttctt tcagttcttc agtaaatatt gcttctttaa tttgctcttt aatttgttgg
     8221 tataatggct tatctgatgt gttagaaatg agcactttca ttttttatca cccccttttt
     8281 atcattttca gtgtgatgtt gttatataca cactatacac ttagagcact tttttgtcaa
     8341 tataaaagat gtaaaatatt actatgacaa aataaatatg aatgaaatat tagagatttt
     8401 tgatagctat aaacactttt taaaaccaca taagatagga tgtattaaat atttattaaa
     8461 agtaagaatt gaatatctaa aaaaaagagg aatcctgtat gaatgatttg tttgatgata
     8521 aattaagtta taaactattt ataaaaaaca taaaggaata aatccatttc taattacaaa
     8581 catactaaaa aatttagata gtaataaata gatagtaata aatgggatga atttataaga
     8641 atttggatag aggaaagagt tattcaagag tgtagactag aggtaaactc tatattaaaa
     8701 aaatttaaat ttggatatta tgatgcatgg aatatttgca gaagtaacct tgctataagt
     8761 atagaagatt tttttgatta aaaaagataa tactctaaaa tgtgagaatt tcaacattca
     8821 atactgtgct ttaaataata tcactgcaaa ataccatcca ccttttacca taattgaata
     8881 ccctaattat tttaaaaaag aaaacattag aaacggaaaa ccttgtatat ataaaataaa
     8941 aaatactaac ttctaaaaca cgaatactct ttgataaata aaacaaaaaa taattttaat
     9001 aattaaaatt ggctatctca aactagagat aagtctcttt ctcactttta aaactttgaa
     9061 aatatattcg atttcgtaca aaaaagagtg ccttagaact aaaaagttca ctttgtggca
     9121 ctcccttttt tctcctcttt ttaattcttt ctaaagattt tccaaaaata ttattaaata
     9181 ccctatttat agataaatca agttaaagtt ttgttttaga aaataaaaat aaactgtaac
     9241 tattagaata gcaaataaaa aatctgtttc ttgataaaat caagattata atgctaaaaa
     9301 gaaaatattc ttataataaa ttaactaata taaaattcgt ttataaatta atactgactt
     9361 tctagcttta tttataaatt aatactactt tttagcttct cctataaatt aacaccactt
     9421 ttttaactat gaaacctatc tacaacattg gtaccataag accagctacc acaatagaaa
     9481 tcatacaagc cataccacca actaccagct taggcataat cttagacatc aaatatgctc
     9541 tttcttcttc cgtttctcca acagcattac acacttcatt tgaaattaaa tatgtagttg
     9601 ggaatcccaa catctgacaa aaccctactc caaaagcaag aggtttagaa ccgataattt
     9661 tccaacctgg taacaccttc atcataagga atataccaat gatagaagct gcaaacatac
     9721 ctacaattgg tacaaaaaga ctaattaaat ctgtcaaaga aacttttgct aaagaaggaa
     9781 taacagctgc aaacacaacc atgttgataa atcccttgat ttttcctttt tccaaaatgt
     9841 cttttggtac aataccaata cgtgttacaa taacaccaac aacaagacat acaatagaat
     9901 aatttacatg aataatctct gaaagttcat accctagata agagaataat gctataaaaa
     9961 atatacaaac atttgtagaa taatatttat cgtatttttc acagaaagct tgttttactt
    10021 ttgtgcctgt attctctgct tctttcttat cagtatcaac atttgctaaa tcaatgcctt
    10081 ttgattttgc ttcatgatat tctactaaaa gacggtttgc ctcttgtaac gctgctcttg
    10141 aagctattgg tgtaccaata aacttctgaa ttgcaaatac aactgttgct gtagctgctg
    10201 caagtgtcag tcctttttct agtgctgctt gtgtcataat agtagtagca ggtaaagctc
    10261 catttataac tggtatgctt gtcaatactg ttgattttcc aaacaaagga atacataatg
    10321 agacacagac tataactgct acaattccaa gccatgctgt caatactgtt ctccattcgt
    10381 ctataaattg tttaacattc aacattgttc ccatactaaa cataatcatt ggaacactcc
    10441 aactagctgc tgcggtcata cctgcttttt caataatatc agctggtatt gcttttgtga
    10501 caaatagaat caaaaataaa agtagtgtaa caaatacagc tgatactttt gcttttgttt
    10561 tgcttgcaat taaatcccca atagcaaata aaactaatac gacactaaac cataataaca
    10621 tatcactcat agaataaccc ccttattata aatatctttc aaaccaatct acaatagcat
    10681 ttgctcttgc aattctatta gatggtcttc catttctaga caatccatgt gtatctccgt
    10741 ggaacaaata catttctgta tctacaccaa gctgttttaa tgctgtaaac atttggaaag
    10801 tatcccccat aaaacatcta taatccctat cactatgtaa gaacaatgtt ggtgtcttaa
    10861 catttttcac atatttaata ggtgaatgat tccaaagaac ctcctcatga ttccaaggcg
    10921 tcttatctcc acaagtctgc ttcataattc tatccccaaa gtcaataatt aaagaagctg
    10981 taatcaagtt actaatacct ctttgagaaa ctgctgcttt gaaacggtct gtatgaccaa
    11041 ttatccagtt tgtcatataa ccaccgtaac ttccccctgc aacccctaag cggtctgcat
    11101 caatcccagg atatttttca atacaagtat ctacaaattc cataagatgc tcatagtcaa
    11161 cataccccca tctttctacc atttctaaga atttttcacc ataaccgtca ccaccatgag
    11221 ggttacaata aatcacatac attccctttg atgttacaca ctgcttcaag tgtgtaaaca
    11281 tatagccata tgcccattta ggtccaccat gaatgaacaa tacagcagga tatttttcgc
    11341 cttctttagc atctataggc ggtataacaa aaccatcaac ttcttcttta ttttttgcta
    11401 caaacgtcaa tttttcaggt aatgaaacct tatgagtatc taaatatggt tcagaaaaag
    11461 cagtcatttt tttcattgta cctgtagtca tatcaatgct atatatttct gctaactcat
    11521 aatctttcaa cattactgtg tacatggtgt tgtcgtaaat atcaaaagat gtaaaagcat
    11581 ctacttttgt gatgatacgt atattacctg tctttgtatc aaactcatta aattctgtca
    11641 tagttctgca aattcttgca ccaataaatt tgtctcctat aactttatta ctacgtccac
    11701 caccatatac tgcatctaca tccagtaatg tagcatacat accatctgga ctagcaatca
    11761 tatttatttc actagtactc aaatcaagta taaaaatatc atgattctgt gtaatagaac
    11821 ggtctaatgt tgaagcttgc aacattactt tatctttgcc aatatatttt gccaaactta
    11881 tttggtactt tccttgtggt acaagttctt tggtttcacc actgtctata tgatactccc
    11941 aaagtccaga aaaaggagtt gcacaatctt cataaatctg accagtatat aaaattctgt
    12001 ttttatcaga agaaacgtct acacctgcta cttcacaaaa ttttggtgta atggcttttg
    12061 ttatattttc accttcttga tataattcta atgttacacg tcttctttga gaaaaatttt
    12121 ctccatctct tataaaaggt ttatctgtaa atgtccaata atctacaccc tctactgccc
    12181 tatctggttc tgatttttta gaatccgttg gattttttgt tgtcagtaac cacaatttat
    12241 ttccaagtga tacaggtacg ctacaagctt ttggaataga aaatgctttt tttgcttctc
    12301 ctccttctat agaaatagtg taaaaatctg ttgtacttgc ttttggctta tttcttttag
    12361 atgtaaaaag tagttctgta ttagaaatcc aactaaatga tgttacatct tttgtttgag
    12421 tcatatgtat ttgtttttct gttttgatgt ccatcacata taagtcatgt tcatagcagt
    12481 ctttttcctc gttgatattt gatataatat aagcaatatg ctttccatca ggtgaacaag
    12541 aaatatcatg agggaatata tattcaaaaa tatctgaact ttcaatcttt attttttcca
    12601 tttttatatt ctcctttcta ctgtcctatg ttatcttttt ttatatatac cgaaaaccaa
    12661 tctagcattt caactaatct ttgtagcttt tgttttggtc taccagaacg agacagttca
    12721 tggttttctc cttcaaatag acaaagtttt gtaggacacc catgaatttt caatgctgaa
    12781 aacatctgga atgcttcttg catatagcat ctatagtctt tttcaccatg taaaaataaa
    12841 gtcggtgtaa cacatttatc agcataagca agaggtgact gtttccattg aagctcgtgg
    12901 ttttccatat gagaagcacc tgtttgtcct ttgcagaaat aataaccaat atctgttgta
    12961 ccctccatgc cactccagtt tgcaatagaa cgctgagcac aagcaacagc aaaacggttt
    13021 gtatgaccaa ttatccaatt cgtcataaat ccaccataac ttccaccttc aacagccaat
    13081 tttgttttat caatgtctgt atatttttca agtacagtat ctgtaaattc cataaaatct
    13141 atataatcaa tatctccaaa ctgtttccta atatctgcaa aagcattgcc acgaccttca
    13201 gacccccttg ggttacagaa gaatacaaag taacctgcac ttgtaaatac ctgtatctca
    13261 ttcataaaat atggtccata tgtcaatctt ggtcctccat gaatcgccaa tacagctgga
    13321 tattttttcc ccacctcata atctactggt ttatatacgt atccttgtat ttcccattta
    13381 gaacttcctt ttgactctat gtattctggc acagaaattt tatattcctc taaaatatgg
    13441 tcatttaaat gtgttttctt acacaatgtt ccattctcta ctgtataaac ttctggtaat
    13501 ccatttccta aacaacccac acatacaata ttgccatctt ttacatctat aaaatcaact
    13561 gaacctgtct gtgttacttt ttcctgtttt cctgttggaa tatgtattct ttcaataatg
    13621 cagtccatat ctactgtaga agtgtaataa atatattctc catctacctt tgttgtctga
    13681 ccagcaccca ttttaacatc agatgttact gtatttccaa tacaatgatg ttgatgccct
    13741 gaaagaagtt tcatttcttt cgtatcaata tcaatagtat aaaaatctcc attttctcca
    13801 tttccataag ataaagcatc agttaggttt agtatcactt gattttgata taattcaaaa
    13861 ccttttataa tatacttatc tttttctaat atacagacat ttgtgtttgt gtttgtatca
    13921 aatacataga ccccattttt caaacctttt acatctttaa actcttgccc tatatataag
    13981 atttttccat cacttatttt catacctaca acattaaaca taggtgctgt aattgccttc
    14041 aactcattta tattactgtc gtatacatat aggcgttttc tttttctgct aatatatccc
    14101 tccccattga cagcataagg aatttctttg aaatgttcat aatgtctttt gttataagca
    14161 tttatttttt tctgttgttc ttcttctggc aatccctcta caataggtcg gtcattgtca
    14221 taagaatcaa caactgcata caaacctttt cctataggat tcaatttacc acctttgata
    14281 gggagtctaa aaagttcttc tgcttctcca ccatttaaag ataaagcata gtaactcatg
    14341 cattgcttac cgtttttgac agcatctttt actttttcat ttcgcaaagc agggaacaag
    14401 atagtatttt catcttccca ggcatatgct ccaacagagt ttatagacgt tatttgtttt
    14461 aattcgccag ttttattgtt taatatataa acttggcttg tgtaaccatt ctctttgata
    14521 tcagcttttt ttacaataaa tgctgtatat cctccatttg gtgatatttg gcaatcactt
    14581 ataaattgat attccaaaaa tgtttcactc gtaaatagct ttttcatagt attttctatc
    14641 ctttctttag taattttttt caaactccct accacctctt tcatttttta ttttaaattt
    14701 aaaataaaaa attttaaatt taaaataaaa aattttataa aggttatttt tttaggaagc
    14761 cttctggctt ccttgtaata tttgtaattt aaagtatatt taatccatgc ttaattcata
    14821 cttaatctat acttagtcca tacttaatct atatttaatt catacttaga cttatttaca
    14881 ctatatttag attatattta ttagattata tttagattat gtttaaacta taattagatt
    14941 atacttagac tacatttagc aacttaatag ataagtatat atagtaactt caaaacttat
    15001 tattcatctt cacagaatct tttaatagtt ccttgaatat gatttcttag tttatctact
    15061 gctttttttg tatctctgtc ttcaatgaat tttaatattt caaaatggtc ttgtactgtt
    15121 ccttcataat tacgattttt taatggtcga attaaatagt tcactactat aaatcttcct
    15181 tgtataccaa catatacatc atttaaaatc tcattatttg ccccttctac aatggcttta
    15241 tgtaattcca aatctgctag tgcaagttct acagattttt cttgacgata gtactcctcg
    15301 catttatcag agtatctttt tattacatct agcttttgtt gaaaaacctc ttcctcttct
    15361 tgcatcattt tttctaaagc atatgtttca atcagtgttc tcgtttcaca aacttgtttt
    15421 accatatcat attcatactc tggtacgtac actttaccat ttgtcataat agtaagcact
    15481 ccatctttat ttagcatttt tactgcttgt acaactggtg ttctgcttac ttcaaatttt
    15541 tcacaaaggt catttagtaa tattttttga cctacagagt attcaccacg aataagactt
    15601 tcaataatct tatgatataa aatttcatct aaatttgact ttactttcac tactctcacc
    15661 ttatttctat tcttttgcca tattatattg aattactgga ctttaattac ttttagtaat
    15721 atttttaaag tagaaattct tatttgatag tacttttgta ctacaattta aattattgta
    15781 ctgtaaatca tgctcttata ctatagatta attcattgta ttgcaaatca agttattgta
    15841 ctatagatta aatcgttata cttcaaatta aactattata ctatagatta aaccattgta
    15901 cttcaaatta aactattata ctatagatta aatcgttgta cttcaaatta aactattata
    15961 ctatagatta attcattgta cttcaaatta aactattata ccatagatta attcattgta
    16021 ttgcaaatca agttattgta ctatagatta aatcgttgta cttcaaatca agttattgta
    16081 ctatagatta aatcgttgta ctttaaatta aactattata ctatagatta attcattgtt
    16141 ctgcaaatca tgctagtgta ctacaaatta aactataaat gtttactatc tcttaataat
    16201 ttgaataata tgttacaaaa agtatagcac ctttttattt ttttcgcaat aaataaaaaa
    16261 tttaaaattt tttatttttt attttcttat attatcaatt tgaaatacga aagattaaga
    16321 ctaatagaat tgatttagtt aattgaaaaa aataggtacc ttttatgaaa attctaatga
    16381 taagcatttt tctgatgtaa tggtaaatta taaatgataa aattaaaaat cacttttagt
    16441 tttatgaact gtccccatat taagaaacaa ctaagataca taaaaatagt tataaagaga
    16501 tagttaaact atatccacta tcttatatag ttaaaatagt gggtatagtc caagctaaga
    16561 ataactaaga aattagcgtt aaatagcata gataaaaata tatttatata aaaacttttt
    16621 ttgctacaaa caattatttc cataacattt tatatacttc tctaaaatct tctaaacttg
    16681 ataaatattc aattaaaggg atataattac ctaaactaca agaatttaag gagtttctat
    16741 acaattcctc actatcccaa gatacaattt ttataggaag attattttct aacatctgtt
    16801 ttagcataac aaatctacct atgcgaccat taccatcttg aaatggatgt atcaattcaa
    16861 atctataatg aaattcagtt ataactttaa tagatacttc actctgacta tagtaccatt
    16921 caattaattc atcaagcttt ggctcaactt caaatggttg tgctatatta ttaaaactct
    16981 aataatatta ataatagctg atataatagc aaatcattat tatcccaaga agatacaatt
    17041 aataactacc ttcctatcca cagtatctag tcaatttcag taactttatg atttagtata
    17101 taatatatac caatcacagt tataccaact ataataaaaa cagaaatcaa actgtcttga
    17161 gttgtatttg taccagctaa aataaattgt cttaaattga taaaaataat tgaactgaca
    17221 agggtaacag gtattgattt tcttatcatt ccaacaacga atgtccatat acctaatgag
    17281 caaaatacaa taatactttt aattgctcct ggtatccaat aagataaaat tgaaatatga
    17341 aaatctccaa taactaaatt aaaatactta tccaacacaa caacgaatgt aaaacaacag
    17401 atatatccaa ctatcattga tgatatcata agagtcgtaa tcaaaagtaa ctttgataaa
    17461 ataagttttt tacggtcaag tggataagag aataaaatca agattgtttt atttgtatat
    17521 tcatttatta tatatgccac attcaacaca gcaaataata taatatagta aaaacagaaa
    17581 actaaactga ttgtcctaaa tgtaacatca taatcatata ttgaagtaga tttatcattc
    17641 aatccagtaa agacaaaaaa catacataag aaaatcccca gtattgaaaa taaaaaatat
    17701 ctttttagcc caactttttt tagctctagt ttcattaaat gtatcatagt gtttctttac
    17761 ctcctgtacc tgtagtttgg aagaagtaat cttctaaaga actttgttta cgtccaatgc
    17821 tatttatacc aatttgattt tgaataaata ttttggatat ttcttttaca tcttttcgta
    17881 aatcatatat ttcaatacaa ctttccgaag taattttaaa gtcttttatt ccaaattctt
    17941 tttctaacaa gtatccagca agctccacat tatctacatc tacttcaaca taatctgttt
    18001 gatatttgtg tatatctgac atagaaattt ctttaataag tttaccatct tgaataattc
    18061 caatagtgtc cgcaatttgt tctacctctg aaagaatatg actagaaata aatatagtta
    18121 ttccataatc tgtatttaac ttacggaata attctcgcat ttctcttatc ccctctgggt
    18181 cgagtgcatt tataggttca tcaagaatca ataattctgg tttagtaaga attgctctag
    18241 ctattgcaag acgttgtttc atccctaaag aatattttga aactttcttg tttgattgct
    18301 ttgacaatcc gacacattcc aatacctctg atatatgttc tttgttataa taacccatat
    18361 attcacagtg taaatcaaga ttctgtttag cagtcaaatt ctcatagaaa taaggatttt
    18421 caataatagt accaatcttt tttaatattt caaaatcagt ttctgccact ttcttactga
    18481 acataactat ttctccagaa tttggcttaa caagatttag caacattttc attactgtac
    18541 ttttacctgc cccgtttggt ccaacaaatc cataaatttc tccctttttc acatgaatat
    18601 taacagcatt aacaacagct ttatctttat atattttctt taattgatgg gtctctatga
    18661 tatagttcat catactccct cctttctaaa aacagtatac aaaataaaat tgtcttttct
    18721 ctcttttaaa tctaactttt ttctaacttt tcaattttac cgtaaaaact gtttttacat
    18781 taggtctact atttacataa attgaacctc ccatagagtt tgcaagccct tgagaaatag
    18841 ccaaacctaa accattgcct ttttttctgt cagcagtata agctcttgtg aaaatatgct
    18901 ctttttcagt ttcagaaata cctgaacctt tatcctcaat atctataaaa acatgttttt
    18961 ccttttcata tagtgaaatc cctaaaaatc taccatctga accatatttt atagcattat
    19021 caattaaatt tttgagtata cgttgtaatg cttctgtgtc aacatttgca tatataggtc
    19081 tatcttcaat attaacttca acattaaagt tttctttatc tagcaaatca taaaactcta
    19141 aaataatctc atgacataat tgtgttagat tggatttttg aagatttaat atcatatctc
    19201 cagattggat ttttgccata ttaaacaggt tatttattga attaacaagc tcatttgaat
    19261 ttgtttgcat tttttccaca gttgctctca ttgaagaaga taattcttct ttttgacttt
    19321 gaagatatag catttcaata taacccttta atactgtgat gggagttcgt aaatcgtgtg
    19381 atatgtttgt aaatatttgc aatatcgctt tttcacttct attataatta atttgcctgc
    19441 tgtaaaactt ttcaagaagt ttatttatat ctactgctgt ttcttttaca atcgaaatat
    19501 cagatggtac taaaatataa ttaatttcat catcatttgt tatgtcacga agtcttgaat
    19561 tgatatactt ataatcactt tccattttct ttgacttatt tcgttctttt aagtagaaag
    19621 caaatagtac aatcgaaatt acaactaaaa ataactccat tattcttcct ccattttgta
    19681 gccaattccc caaagagttt taatataatt atttttttcc ataccatcag acaatttatt
    19741 tcttaacctg ttaatatgag tatttagcac attttcattt ccataataag gttctttcca
    19801 aatagcgttg tataattgtt cctttgaaaa agcacgtcct tgattagtcg ctagtaattt
    19861 aacaatttca aattctgtat gtgttaagtt taagtcaatt ccattctttg aaactacata
    19921 ttttgatatg tttataatca aatctttata ctgaataacc atatcttgag agtttcttga
    19981 actgctgtac actgttgcac gtctaatatt agctttaatt cttgcaagca attctacgat
    20041 tgaaaaaggc tttgtaatat aatcatctgc accaagatta agccctagca ttttatctgt
    20101 gtcactatct ttcgcagtag taataataac aggaacaaca ctcacacttc gtatatattt
    20161 taatacttca aaaccactag actttggtaa catcaagtcc agtaaaataa tatcaaatga
    20221 ataattttta aatatttcaa tagctgtatt tccatcaatg gcagttttaa cagagtaatt
    20281 ctcactaatc agaaaatcgt ataccatttc tcgaatattc ttatcatctt caaccaataa
    20341 gatgtccatt attattcaac tccttttcgt cagtatactt caaatgatat atttattaaa
    20401 aagggttgtc aataaataac tgcttatgat attgtatctg caatttggtg atactcttct
    20461 aagttaaagt tcttttcaat aagctttatt gaaaaaataa ttaaaatata gtatactctc
    20521 ttatctttat ctgaaatata cataattttc ttttattaac atctacaatt aactatttta
    20581 tattataaaa caccttttaa cttttgctaa aaaatatttt atacataaaa aagaaaccac
    20641 attctagttt tatacagaac atagtttctt taaaaataat caaatttgga ttttaaataa
    20701 ttaaaaacaa acttttattt gtttttaatt ttccaatttt atagtagata atttagttaa
    20761 ttagttctta aatgtttatc ttctttagta aatttttttc ttattaagaa atatgcaact
    20821 attacaacaa gttctattgc taaaaataca ccataagtag ctatagaatc tatgctatct
    20881 atgccatttc taaagaaaat taaagcaatt cctgctgcta taatacctac agcatttttc
    20941 cctatagaat tttgaccaaa accataaaaa gaagggaaat ttccttttat ttcaatgaat
    21001 gtcaagatta atccaatagc tataaatagt actcctacaa cttccataat catactagtc
    21061 attttgaccc ctccttataa ttaactacta ctataaaaat atttattatt tttataatta
    21121 tattatatat gcgattacat gtttttggaa gtatatttgg aagtaatttc atatttattt
    21181 aacttaattt ttttgtgata aaataattct aattttcata tagatattat tataaatttt
    21241 ctagtttatg gagtcattca ttctataatt cacaaatatg tattttttaa tctattaaag
    21301 tattcatttt ctggcttata agcatggact tcttatctat aagcttgttt tttcatctaa
    21361 taagttattt attctttaat ctatgagctt gatttttcat ctataagtca tttatttttt
    21421 aatctatgag tttggttttt catctataaa tcattcattt tttaatctat aagcttgatt
    21481 tttacctata attaattcat tctctaatct ataagcttgc ttctttatct ataagtcatt
    21541 tattctctaa tctttaagct tacttcttaa tctataaatc attcattttt taatctataa
    21601 gcttgatttt tacctataaa tcatttattc tctaatctat aagctttctt tttcatctat
    21661 aaatcattca ttctttaatc cattagcttg gtttttcatc tataagtcat ttattctcta
    21721 atctataagc ttggtttttt acctataatt aattcattct ctaatgcata agtaatacat
    21781 gaaatttaaa tattattttc ttcaataaat tcaaacaact cttctacagt tgtatcaaat
    21841 accttagcaa tatccattgc cagttttaaa gaaggattgt agcgaccatt ttctaaatgt
    21901 acaatagttt cacgtcttac tcctactgac tttgcaagtt caccttgtgt cataaggttc
    21961 ttttctctat aggatttaat ttttgtcttt agtaatactg acacctaatt gtatatcccc
    22021 tttctgtcaa aatatataaa tagaattaac ttcaacaagg attgaataaa caaaatgact
    22081 aataaagaca ttcctatatt taaatttgac acatttatgt ccaaagttac aagaaatata
    22141 gatactatag ctacagaaaa aaacaatact tttattgcaa tatcattaac tcttgatagt
    22201 atacattttg atgcctcatc aattaaatca tcatactttt tattatagac ccactgagct
    22261 cccatagcta atacccaaat aattatagga agatattcaa tactacttcc tttaaaaaaa
    22321 tacttattta ctaatgtagc tccaatacaa taagaaattc ccaatatttc tgaaataagt
    22381 gttgatttaa taccagtttg ttgtttagac atataaaatc ccccttaaaa agttatgtat
    22441 ttataacacc tcttgttata aatatataac ttttttgaat aaatttcaat aaaaatatta
    22501 ttttgcaaca aattctttta ttaattttac attagataga ggcactttag atgtcactcc
    22561 ttgcaatgtc aaatcttcta tattaacttc atttaatttt gaacacagtt ctaagtcaaa
    22621 tattctttcc caacttacct tcttttcaat ttgttcatct tcataaagct ctacagtaat
    22681 atccattaaa ggtaaatgcc ataaatcaaa ttctgataat aacacattgt cttcacttat
    22741 ttctatttca agtaaaacac ctttttttcc tttttctaaa taaccactac atcttaaatc
    22801 aggtctcttc gtccaaaccc atatcatact atcatcaaat tcatatattc tatttgtcat
    22861 ttgactatac atccatttgt atggaatatc aaagtcttct aatataaaag aataatcatt
    22921 tgatagatat ttattgtttt tattgttatt ccaaaattct aaagtctgta tagtataaaa
    22981 tttcatatat aaatcaaccc ctttgttttt ctataagaag gagatggttc aaacttttat
    23041 tttgaattat ctcctttatt tatacacctc tttgtaccta ttatttatat atctctttat
    23101 atctaccata tttaaataac aaagttttat attaatttaa atttaaaaga ggaagctttg
    23161 tatttatcta gtgccatctc aatcatagta ttagttttga ttatagactt tttcaacctc
    23221 tgatttattc aaatcatcta taatatcgca tatttcactc aaattgtatc ctttattttt
    23281 taattttatg atgatattaa atttctcttc tcttttccct tttgcaatac ctttttcgat
    23341 accttcttga aaaatttctt ctttaaaatt tctactttta ttcataatat acgcacctct
    23401 ctttaaattt aataaatagt cctcattact acctaattct tttaatctat caagtgctct
    23461 tttaataaca taatccgttt tagatagtat tctaatatct atatgagaag gattatcaat
    23521 aagtttaccc catattagaa ctctgtttgc tatacaactg gatttacgaa tttttggaag
    23581 ttctataaaa tataatgcag ttttactaga aaaaatatct ggatgcccgt ccattgttaa
    23641 tctcaggcaa tttacataat cttctatgtg attatatttt acatgattta gtatatgtat
    23701 tccaacagtt tttccaattt ctttatattc tttaccttct tctaactttc taccatgtac
    23761 acgactaaga taatattcag ctctttccaa ataatcctct tcaagagctc tttgcatctc
    23821 aatatttata tggttacgtt cttcatcttc agccaaaata tcaagtctag gtcttttctc
    23881 accaatgtaa ttaattgttg ggtcggtttc aaccttaatt attcttttta tttgagtgaa
    23941 ctgtttgata ttcctattag catatggagc atctttgagg atatcattta taaaatttgc
    24001 aagtaaatcg taatcattat ttaaagtaaa caatgattta aatacctcat catactcagg
    24061 cttagcatac ccatcttcag ttacaacttc tccaacattt tcactagtca atatttcttt
    24121 tggaattaaa tccttgattt tatctaattt ttcattagaa aaatcacctc tactaagtat
    24181 gtcatttaat tttttggtcc attctctgtc cttttcttca attatagttc gtttatactc
    24241 ttttgatgtt ttaataatct tagatttttc ttctaatttt tctttcatat caacacctct
    24301 tttttattat tacccatttc ttcataccaa agtctgaact tctaagtaaa atttgcttta
    24361 ttgaaataag aaccttataa acttatatat cattactaaa gttaagactt taaagagaag
    24421 cacttagatt acaatagatt ttagatagct ttctttttaa aactattgtt gataaatgaa
    24481 tgaatataag ggtatgtatt aaaatagaga tactcatctt tttcattgtt taagtaatga
    24541 aaatatattt tatttaatac aaaaaagagt gccttataaa ctaaaaagtt cacttcgaga
    24601 cactcactta cgttttgttt cttaaacttc acaatattac atacttattt attaatttaa
    24661 tctatgcata cttaacccaa taataaagta catatatcca actcattaag ccatgaaaaa
    24721 tagcccatgg aatagatttc catgcagtat aagaaataat cattgctaaa caagaaccaa
    24781 aagaaatacc ctttttaaca gttgatttta tagtattatt atttgaatcc ataattaatc
    24841 ctccaaaatt caaaataaac aagttattat atatcttatt tgaatctaat acaatataac
    24901 agcttctaaa taaattatac tcttattata cataatttat aaattataat tccgataatt
    24961 atatgaatta attaattata tctacctaaa aatattatcc tatctaaaag ttatatgata
    25021 gatacaacat gtcaacacat tgtaattctc cttcaaagat tggttctgga taattatcta
    25081 caaaaaagtt tttaatttta tgtgaataaa caaatccaaa cttctcataa aaaggaactc
    25141 cgctttcaga tgtaccaaca accattgatg aatatttttt actataaaca tctaaaagat
    25201 gataaatcat ctttgaggca taacccatat tttgaaaatg ctcatatgta gcaatgtttt
    25261 ttaattcaca tatttctttg gaaatttcat ttacaactgc tatacaagta agttctttat
    25321 tatcatacaa tgcgtacata gttcctttat tgagatattt atcaatcatt tcttcacatg
    25381 ggtcggctaa cattaaaagg tcaatttgac tttttttatt tatttctata gtttttattt
    25441 ctatcataag ttctccaccc ttactttatt caaaccttaa tttagcaaac ttcttacagt
    25501 acttatttag ttcattttga tatatatttt tactttttta aaatatagtg ataattccct
    25561 agaatattaa tattaataat aaattataat atcagctcta gcagttacct agaatatcaa
    25621 tactaatatt catctataat atcagctcta gcaattacct aaaatatcaa tactaatatt
    25681 catctataat atcagctcta gcaattacct agaatatcaa tattaatatt catctataat
    25741 attagctcta gcagttacct agaatatcaa tactaatatt catctataat attagctcta
    25801 g
//
LOCUS       DBGJFF010000054        24586 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.054, whole genome shotgun sequence.
ACCESSION   DBGJFF010000054 DBGJFF010000000
VERSION     DBGJFF010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24586
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.054"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(<1..876)
                     /locus_tag="ACOIAF_002128"
     CDS             complement(<1..876)
                     /locus_tag="ACOIAF_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HGZ2669137.1"
                     /translation="MNTTKKLTEAALLSSLFIVVTIIAVSTGFGYAIYLDFIVPVFFC
                     IICLKCDLKYTILSGITSLLIISLVLGNLGTAIWASQSVLLGIMCGYLINKPTMVMDD
                     LVYGSVFGVIVMVFIDIYASTLIGYSFMKEFQGYSKWIYFNGYTNFIYYLMIALFPFG
                     MVFCIYLLSLILGNKLHILDSNSLKKFLIFKNFRNLNQFLCCSKKAFYICVSYLAIFE
                     VLKLLNVKVDSVYLKTIFISITYISVYFIIRDSCMSLQNFIIAKFRKLLYARILFLII
                     ILLLFFIFDVTVIGLI"
     gene            1049..1489
                     /locus_tag="ACOIAF_002129"
     CDS             1049..1489
                     /locus_tag="ACOIAF_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGZ2669138.1"
                     /translation="MFKIKHFNDLSLDEFYEIAKSRYEVFACEQKIFSLNDYDDIDKS
                     SYHIFLKENGLICAYARIIPKEYSSYNDVSIGRVLVLSSHRRKGLAKQMMDCAIDFIK
                     VNLHENNITLSAQTYIKNLYLSCGFKEISEVYDEAGIEHIKMRL"
     gene            1816..2043
                     /locus_tag="ACOIAF_002130"
     CDS             1816..2043
                     /locus_tag="ACOIAF_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HGZ2669139.1"
                     /translation="MKNLNDIQVKKTVKVEDILSSGNLRERMLALGLTRGAVIDVVRK
                     GPKNNLTVYKIRGSKIALRQEESSLILVSDI"
     gene            2069..4189
                     /gene="feoB"
                     /locus_tag="ACOIAF_002131"
     CDS             2069..4189
                     /gene="feoB"
                     /locus_tag="ACOIAF_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HGZ2669140.1"
                     /translation="MGLTHNSTKMSSLKDMFDIDNKEDQFVIALAGNPNTGKSTVFNH
                     LTGLRQHTGNWPGKTVATARGNFKYKNTEYALIDLPGTYSLFALSQEEIVARDFICFG
                     NPDAVIVVCDATCLERNLNLVFQVMELTDKVILCINLIDEARKKGITIDKKLLEDSLG
                     IPVILTAARNGSGMDELLDTLNDVSFDKYKLNNKPVRYNENIENVVKSIQPELDNIIP
                     GINSRWLGLRLIDGDESIFESMSNYIDKDSIDAINEVKKKIPDNINKQKIRDEFTKIN
                     YDYAKKLSDECCSNVAKKSTDREEKVDKILTSKIFGLPIMLLLLGTILWITIEGANYP
                     STLLSNLLLGFEPSISGILNSINCPSWLNDMLVLGLYRTLAWVISVMLPPMAIFFPLF
                     TLLEDFGYLPRVAFNLDHLFKKACAHGKQCLTMCMGFGCNAAGVIGCRIIDSPRERLI
                     AILTNNFVPCNGRFPTLIAISTIFFSSVITNSFVSSVATALCITLLIILGVIITLLVS
                     YTLSKTLLKGVPSTFTLELPPYRVPQIGRTLYTSIIDRTIFVLGRAVMVAIPAGVITW
                     IFANIYIGDLSILSHVANFLDPLAKLIGLDGFILLAFILGFPANEIVVPILLMAYLAT
                     GSMIELDSFSALGQVLREHGWTYLTALNVMLFSLLHWPCATTLLTIKKETGSLKWTAL
                     GFLMPTILAFVVCFLTTTVYNLFI"
     gene            complement(4322..5140)
                     /locus_tag="ACOIAF_002132"
     CDS             complement(4322..5140)
                     /locus_tag="ACOIAF_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HGZ2669141.1"
                     /translation="MEKQYFTTGEFAKLCGISKQTLIFYDKIGIFSPEYKDKNNYRYY
                     SVYQYDTLDILQSLREIGMSLEEIKEYIQNRTPQLCVKMLKEEEKKIREKIKKLRKIS
                     SKMQNRINITVEGISKMNSEEIYISKKFEEYLVLSSDLSNMSNSDFMMELIDFTNYCK
                     SKNLYQGYELGVIVSNENIKNGDYLSISSFYLKIDKKIKDKKLYVKPEGMYACIKHIG
                     KYEDSYKSYEKLKKYIYENEYKIIGNSYEESILDFFCESNEDNYMTEISIQVSR"
     gene            5359..5631
                     /locus_tag="ACOIAF_002133"
     CDS             5359..5631
                     /locus_tag="ACOIAF_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669142.1"
                     /translation="MKIVIGATIRYLIKHKVNYCNKKSLDKLELKYGNIDREKAVKLE
                     IFYQYLIELEYIEMSLLIRRLDTAITSLALVSIITIASYYLIRKNV"
     repeat_region   5799..6289
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=5799..5827
                     /rpt_unit_seq="atttacattccatatagttaatataaaac"
     gene            complement(6948..9041)
                     /gene="pdcA"
                     /locus_tag="ACOIAF_002134"
     CDS             complement(6948..9041)
                     /gene="pdcA"
                     /locus_tag="ACOIAF_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdcA"
                     /protein_id="HGZ2669143.1"
                     /translation="MNKHNFEVILNQLQINIYVTNIHTNEIIFMNKKMKEEYNILDPE
                     GKVCWQVLYPEKNSTCSFCKVLELLKNDKKGVLIKWYEKCNKLNRVFENYDSLITWQD
                     GTVVHMHQSIDIANSTSLNKPIKINEFHEISNNKEEKGVFNFSRDNFDYNSTLLYDAL
                     IRGTDEYIYICNMKTGVFRYSPSQVELFDLPGEIVKNPLVYWKKIVHPEDWNRFYKSN
                     TEIGKNQMDYHTVEFRAKNRSGEYIWLRCRGQLMRDEFGEPSIFAGIMTQLGKQNKID
                     SLTQLLNYHEFMSVFEDKISNPMIEKLCIVLLDIDDFKNVNEMYDRDFGDNIIKTLAQ
                     SIQSILPDNAELYKLDGDEMGILVDNVEENEILTLYNQIQNMIIHLQLWRKYGLNITI
                     SAGCVIYPKHGDTVKELYKCASYSLQYAKEHGKNRLVFFSQEILKNKMYSLEMMRDLK
                     ASINDDFRGFSLRFQPQVDTESHKIIGVEVLLRWTNDKCKAISPLEFIPILEENDMIN
                     IVGAWVLRMALRTFRKWIDYYPFFKVSVNVSAVQILEDTFIEDIVKIIDDENFPYQNL
                     VLELTESHTVQNMSILQFKFKALQDLGIYIAMDDFGTGYSSLEVLKFSPIDIVKIDRV
                     FVKDILKSKFDATFIHFIVAICHDVGIKVCLEGVETQEEYDLVKQIKPDYIQGYLFGK
                     PQTATEIFDLLKLDN"
     gene            9685..10617
                     /locus_tag="ACOIAF_002135"
     CDS             9685..10617
                     /locus_tag="ACOIAF_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006441199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rossmann-like and DUF2520 domain-containing
                     protein"
                     /protein_id="HGZ2669144.1"
                     /translation="MGFIGSGKVGTSLANYFLSKGYCVSGFYGRNQLSLLESTNLTKT
                     KIYSNLKDIIYENDILFITTSDDSIEIIDKKLSKFNLKHKYICHTSGSLKSSILFCSK
                     KAGALIYSIHPIFAFSNKNTNIKKMKDICFSIEGDNEQDDLVIQNFIDDLGNQFFIRD
                     KDTSSTYHLANVLVSNLVLSLLDIGVSYFIKLGLSEEESLKAISPLVKKNIENILDNG
                     FTKALTGPVSRGDITPIRKHLSVLDKKDEDIYKILSLNLLKIIALGNDNSLKGIKNIT
                     IENAIENLISKSEKYKEIYKLLGGKNDEKYHTDF"
     gene            10592..11419
                     /gene="panB"
                     /locus_tag="ACOIAF_002136"
     CDS             10592..11419
                     /gene="panB"
                     /locus_tag="ACOIAF_002136"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HGZ2669145.1"
                     /translation="MKNTIQTFKNAKYEGKKLSMLTAYDYSIAKIMDECDINGILIGD
                     SLGMVIKGEENTLSVTIDEIIYHTKAVKNGVKNALIVSDMPFLSYHVSIEDAVKNAGR
                     LIKEGGAHAVKLEGGSNVIKQIESIVNAQIPVMGHLGLTPQSVNSFGGFKVQGNTSET
                     ARQLIEDAKLIEKAGAFSIVLEGVPTKIAEMVTNSISIPTIGIGAGINCDGQILVYQD
                     MLGMFGDFVPKFVKQYANIGDIMKDSIKNYILEVNTGAFPQEKHSFSINESELEKLY"
     gene            11438..12286
                     /gene="panC"
                     /locus_tag="ACOIAF_002137"
     CDS             11438..12286
                     /gene="panC"
                     /locus_tag="ACOIAF_002137"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HGZ2669146.1"
                     /translation="MLVKEIKLLRNIIKDWRKHGYSIGLVTTMGFLHEGHQSLIKKAV
                     KENDKVVVSVFVNPTQFGPNEDFNSYPRDIDKDFKYCMDSGATVVFNPSPEEMYLKGN
                     CTTINVSGLTDFLCGAKRPVHFGGVCLVVSKFLNIVTPDKAYFGEKDAQQLAVIKRMV
                     KDLNIDTEIIGCPIIRENDGLAKSSRNTYLSEEERKSALILNKSLSLAKEELVKGNLN
                     PENIKELITAKINSEHLAKIDYVEIVDSETLQPVKQIEHSILVAIAVFIGKTRLIDNF
                     TFELNI"
     gene            12716..12898
                     /locus_tag="ACOIAF_002138"
     CDS             12716..12898
                     /locus_tag="ACOIAF_002138"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669147.1"
                     /translation="MFKDKIDECVHIMTAYIASLKEYYSFIETQIGDFIKKYGEDVVE
                     LCLHRVMILLCECGLA"
     gene            13730..14644
                     /locus_tag="ACOIAF_002139"
     CDS             13730..14644
                     /locus_tag="ACOIAF_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2935 domain-containing protein"
                     /protein_id="HGZ2669148.1"
                     /translation="MIDNQKYVILSLELHLFFSRIMKEHALFLEAGFTNKNYNLAMEA
                     DHYKKQFEDLLSYTVSASNGIIRPDILYSEELVTTLTSVAEQKTEEFTGIEINKNITT
                     RELNLQSGVNPQVGQDLVNYVAQLNSDAIRLLDGLINFKERVLDGVLSCTIFTSNYPL
                     LLEHIIHEANLYRSYVVDLENKIDIESKNAKEIELFWDHIMMEHALFMRGLLDPSEGE
                     LINTSNDFAIKFNELIEKTNEMTDSNIKNITEETLNETVEFKDFKEAGASGIEQCKIK
                     SIILPLLADHVLREANHYIRILESYKNM"
     gene            15034..15681
                     /locus_tag="ACOIAF_002140"
     CDS             15034..15681
                     /locus_tag="ACOIAF_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HGZ2669149.1"
                     /translation="MIRQTPLEKRYDKLGPHIVTALKKRYFDAYYCKTRNEALQQILD
                     LIPQNHVVSWGGSETLKEMGVQTAVREKGFKVIDRDLAKTPEERTEIMRQALLCDTFL
                     MSSNAISEDGQLFNIDGNGNRVAAMIFGPKNVIVVAGMNKIVKNIDDAIQRTRTVASP
                     AVVQRFQDVNTPCYINGSCIDCTSPESICAYMVTTRISRPAKKIKVILVGENLGL"
     gene            complement(15848..16417)
                     /locus_tag="ACOIAF_002141"
     CDS             complement(15848..16417)
                     /locus_tag="ACOIAF_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HGZ2669150.1"
                     /translation="MKKFIICIIMFAISVLVLAGCTNDNKIKNEVDSMFNSIKTQDAD
                     TVDKYFPKSGLGNLIYEDKEGFKMYSKNMTWEISDIKNNKDKVTVDLKINNTDMVSIL
                     KKNPPKLGEMMPNPTMKDIDSAKKKEFNITLELVKSGDNYVCKVNQESAMKLLNVITG
                     GGIDYLADYNKDYYKQLDEEQKNYENKNS"
     gene            complement(16422..17321)
                     /locus_tag="ACOIAF_002142"
     CDS             complement(16422..17321)
                     /locus_tag="ACOIAF_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HGZ2669151.1"
                     /translation="MELLSKVNNNIRLITQVAFTALTNGYVNGFLEGTIYGGESKKLC
                     VPGLNCYSCPGALGACPIGSLQAVLSSREYKFSFYVVGFLMAFGAFFGRFVCGWLCPF
                     GLVQDLLHKVPFPVKIKKVYGDKYLKYLKYIVLIMFVIILPVAIVNVAGGGNPWFCKW
                     ICPSGTLLGGIPLILGNENLRESIGFLFSWKLSILLLILLMSIITYRPFCKYICPLGA
                     IHGFFNPISIYRFKIDKDKCTNCTACQTKCKLDIPIYKDVNSPDCIRCGECIKVCPQN
                     AITTTFSKDKKENNICAKKYRGI"
     gene            complement(17332..17481)
                     /locus_tag="ACOIAF_002143"
     CDS             complement(17332..17481)
                     /locus_tag="ACOIAF_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1871A family CXXC motif-containing protein"
                     /protein_id="HGZ2669152.1"
                     /translation="MSNFIKDNKKGISIIAISIIFIALGTYRGETNTVLTKAIMICLE
                     CIGIG"
     gene            complement(17474..18058)
                     /locus_tag="ACOIAF_002144"
     CDS             complement(17474..18058)
                     /locus_tag="ACOIAF_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HGZ2669153.1"
                     /translation="MKRFFIVVLGCICVLGMISGCSKKESKPVEKNKNNSEWFSNLDT
                     EDVDGNKVTKEIFSNKELTLINVWTTWCGPCVGEMPELEALSKEYESNNSNVAIKGLV
                     VEVDKTDMRTGLSNEEKNLVKDIMKKSDATYQQLTVSEELKKTDFKRVMEFPTTYFVD
                     KKGNFVGEKVVGANSKEDWKKIIDERLKMVKANE"
     gene            18447..19772
                     /gene="cdeA"
                     /locus_tag="ACOIAF_002145"
     CDS             18447..19772
                     /gene="cdeA"
                     /locus_tag="ACOIAF_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter CdeA"
                     /protein_id="HGZ2669154.1"
                     /translation="MENLFTRKFTTFEFLKFVSPAIISMIFISLYTIIDGIFVSTLVG
                     SDALASINIVLPIINLVCGFGIMMATGGGAIVSIRMGENRQDEANSTFSFIVLFSLIV
                     GILFTVISYFFIKEISILLGATDKLLPYCITYGKVMILCTPFYILKFIFEYFARTDGN
                     SKFSLFLSVIGGVTNIILDYVFIKYFGMGLLGAAVATAIGIILTCVLGIIYFLSNKST
                     LKLRKPKTDFRLIRDTMINGSSEMVTELSTGITTFLFNVVALKLAGENGLAALTIVLY
                     AHFLMTSVYLGFAAGVSPLISYNFGAENSDKLKETFKHSLKFIFISSLLVFIIALVFA
                     PFIVRVFVNPDNTVFKLALQGLKIFAFAFLFVGINIFASGFFTAFHNGKISAIISFSR
                     AFVFIIIGIIILPPMLNMTGLWLTVPFAEVITIFISILFIKKYKGRYKY"
     gene            complement(19867..20661)
                     /locus_tag="ACOIAF_002146"
     CDS             complement(19867..20661)
                     /locus_tag="ACOIAF_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2669155.1"
                     /translation="MNELKPYIKFFNKNTKLYIIIFMIVGIVMNILPVFILKIFGEAS
                     KTNLSTIVNPILTYITILLFVYGVNIANKDFSGALSIRADRKSCIKAIVIDLIILSFV
                     ISILGIVLIFLSKLFIEIITGYSIELSVLLKRDMIWTSISALIDPSTSSMPLTYLNYF
                     VQICINEICIGFIGVLLGAFTYRVRKVTSITILIGLPILMVIYIVNFATKNMHKMLLY
                     LEKIIYSFQNPFILFGTKVGIIVICIIFIILLLRKAPIKEYANDLL"
     gene            complement(20662..21537)
                     /locus_tag="ACOIAF_002147"
     CDS             complement(20662..21537)
                     /locus_tag="ACOIAF_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGZ2669156.1"
                     /translation="MSSSIKIENLSHSFKKQNIFNNMSLNFEKNKIYGLLGKNGAGKT
                     TLLNIMVNQLIQNEGEIEILGKNPKEDIKVLEEVCIVREKEFYSPDFKVGQIFEFSSS
                     FYKSYDKGLEEELCKYFDLNTKKKYKQLSRGMKTILSNIIGICSNSAITIFDEPTIGL
                     DAVNRQEFYNIILDSYIKNPRTIIISTHLIDEIDDLLEHVIILNEGKIIIDEEIDVIK
                     QKAHYITGNKEELEKLECIKNIKPKKAFGNAVAYFYYGDFSENDEKILKNSNIDVGYI
                     GLQDMFVNMTKKEEL"
     gene            complement(21541..21909)
                     /locus_tag="ACOIAF_002148"
     CDS             complement(21541..21909)
                     /locus_tag="ACOIAF_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGZ2669157.1"
                     /translation="MKLVLNNEEPIFIQIARAIEDEILSNGIKEEEQVPSTTELSKLY
                     KINPATVLKGINILVDKNILYKKRGIGMFVSDGAKTIIKEARKENFKHNFVKNLLQEA
                     NKLEINREELVDIIINFKED"
     gene            complement(22091..22750)
                     /gene="deoC"
                     /locus_tag="ACOIAF_002149"
     CDS             complement(22091..22750)
                     /gene="deoC"
                     /locus_tag="ACOIAF_002149"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HGZ2669158.1"
                     /translation="MKHILKTVDHTILKATTTWEDIKILCDEAVDMSVASVCIPPSYV
                     KRASEYLKGKIKICTVIGFPLGYQTTATKVFEAKDAIENGADEVDMVVNISDIKNKDY
                     DNIGKEIKEIKKAIGDKVLKVIIETCYLDEDEKIKMCEIVTMSGSDFIKTSTGMGTGG
                     ATLEDIKLMKEHVGKNVKIKAAGGVKSISDAEKFIEAGAERLGTSSICKILKNEDTTD
                     Y"
     gene            complement(22786..23973)
                     /gene="murJ"
                     /locus_tag="ACOIAF_002150"
     CDS             complement(22786..23973)
                     /gene="murJ"
                     /locus_tag="ACOIAF_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HGZ2669159.1"
                     /translation="MKQEPCDFSHGRFRTGIIFIGITSVMSAFLQIKENFIVVGFGSI
                     PYNIVIIISIMLSTIFGPYILPIGAVVAMVVQLLFYMFFVKKTNYKYLYYLNFKDDSL
                     IKLLALLSPVFIGVAVNQVNSLVDTTLASTLVKGSIPALTYADRLNGFVTGTFTASIV
                     SVMYPMLSKLSAENNQKKFTSSVKSSINMIIISMIPISVASIFFATPVVRIIFERGAF
                     DARATQMTATALIFYAVGMTAFGLRDILGKVFYSLQDTKTPMVNGIISVGVNIVLDLV
                     LIKPMAHGGLALATSSSSIACILLLFLNLKRKVGYFGQDKIIKATLKSVVASLIMGVL
                     SYFTYKFIFGILGVGTFNEFVSLAISVIVGGGIYTLLMTIFKVEEVDMILNIAKRKLH
                     LKK"
     gene            complement(24050..>24586)
                     /locus_tag="ACOIAF_002151"
     CDS             complement(24050..>24586)
                     /locus_tag="ACOIAF_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HGZ2669160.1"
                     /translation="SCGEFIENPKYLKKSLNKLAKLQRELSRKTIGSLNRNKTRLKVA
                     RLQEHIANQRNDFLQKLSTKLIKENDIICIEDLQVKNMIRNRKLSRLISDVSWSEFIR
                     QLEYKANWHGRQIVKVGKFFASSQICNKCGYKNEEVKDLNIREWICPSCNETHDRDIN
                     ASRNILKEGLRLITIQNK"
BASE COUNT         8882 a         3537 c         2933 g         9234 t
ORIGIN      
        1 tattaaacca ataactgtta catcaaatat aaaaaacagc aataaaatta ttatcaaaaa
       61 taaaattcta gcatataata attttctaaa cttagctatt ataaagtttt gtagtgacat
      121 acatgagtct ctaatgataa aatagacact tatataggtt attgatataa agattgtttt
      181 taaataaaca gaatcaactt taacattcaa cagttttaga acttcaaaaa tggctagata
      241 agaaacacat atgtaaaaag ctttttttga gcaacataaa aattggttta aatttctaaa
      301 attcttaaat attaaaaatt tttttaaaga attagaatct aaaatatgca atttattccc
      361 taaaattaaa cttaataaat atatgcaaaa caccatacca aaaggaaaca atgctatcat
      421 taaataataa ataaaatttg tatacccatt aaaataaatc catttgctat atccttgaaa
      481 ttctttcata aaactataac ctattaatgt tgaagcatat atatcaatga aaaccattac
      541 aataacccca aatactgaac catatactaa atcatccata accattgtag gcttatttat
      601 tagatagcca cacattatac caagtaatac actctgactt gcccaaatag cagttcctag
      661 atttccaagt accagagaaa ttattaaaag cgaagtaatt ccagataaaa tggtatactt
      721 taaatcacat ttcaagcaga ttatacaaaa aaatactggc actataaaat ctaagtaaat
      781 agcatatcca aatccagtac tcacagctat aattgttact actatgaata aagatgacaa
      841 taaagctgct tcagttaatt ttttagtagt attcaaaaca ctgcctccat aaatcataaa
      901 ttttctaact aaagttttgt ttttaatata gtatatcact ttttaaaaat atttttaata
      961 aatataaatt ttaataaagg tatttatgtt aaaaatggta taatgtatct taatattcag
     1021 ttttttaaga tgctaggagg taattactat gtttaaaatt aaacacttta atgatttgag
     1081 tttagatgaa ttttatgaaa tagcaaaatc aagatatgag gtatttgctt gtgaacagaa
     1141 gatattttca ttaaacgatt atgatgatat agataaaagc tcttatcaca tatttttaaa
     1201 agaaaatgga ttgatttgtg cttacgctag aataattcct aaagaataca gctcttataa
     1261 tgatgtttcc attggtagag ttttagttct ttctagtcat agaagaaaag gattagcaaa
     1321 acaaatgatg gattgtgcta tagattttat aaaagttaac ttacacgaaa ataatataac
     1381 cttatcagca caaacttata taaagaacct ttacttatct tgtggtttta aagaaatctc
     1441 agaagtatac gacgaagcag gtattgaaca tattaaaatg agattatagt gttgacaagc
     1501 taatattgtt aatatataat ttaagtataa attaaatatt gaattgatat ttaggtattt
     1561 gaaaatacct tacatagagt cagaaggctt ttgctttctg acttttttat tatcaaaatt
     1621 caataattat atggattcaa agtgttaatt tttattctat attctacttt ccccaaaagt
     1681 aagaattata cccaactttg tatgataggc atcttgccta tctttttttt gtaaaagtaa
     1741 caaggtaata atataatgca tagtttaaaa taagaagatt agcccaggct aaatttcaag
     1801 catggaggtg ctattatgaa aaaccttaac gatattcaag tgaaaaaaac tgtcaaagtt
     1861 gaagatatat tatctagtgg taatttaaga gaaagaatgt tagcgcttgg tcttactaga
     1921 ggagctgtta tagatgtagt tagaaaaggt ccaaaaaata atttaactgt ctataaaatc
     1981 agaggtagca agatagctct acgacaagaa gaaagtagct taatattagt ttctgacatt
     2041 taaaaatttc tagcaggagg atattaatat ggggttgaca cataactcaa ctaaaatgag
     2101 ttctttaaaa gacatgtttg atatagacaa taaggaagac cagtttgtaa ttgcattggc
     2161 tggcaatcca aatacaggta aaagtactgt atttaatcat cttacaggac ttagacagca
     2221 tactggaaac tggccaggaa aaactgtagc tacagctcgt ggtaatttta aatataaaaa
     2281 taccgaatat gcattaatag atttaccagg aacatattct ttatttgcct tatcacaaga
     2341 agaaattgta gctcgtgatt ttatatgttt tggtaatcca gatgcagtaa tagtagtatg
     2401 tgatgctaca tgtcttgaaa gaaatttaaa tttagtattt caagtgatgg aacttacaga
     2461 taaagttatt ttgtgtatta atttaataga tgaagctaga aaaaaaggca ttactattga
     2521 taaaaaactt ttagaggatt ctcttggtat tccagttatt ttaactgctg caagaaatgg
     2581 ctctggtatg gatgaactat tggatacttt aaatgatgta tcttttgaca aatataaatt
     2641 aaataataaa cctgttagat acaatgaaaa tatagagaat gtggtaaaat caattcaacc
     2701 agaattggat aatattatac caggtataaa ttctaggtgg ttgggactta gacttattga
     2761 tggcgatgaa agtatatttg aatctatgag taattatata gacaaagatt ctatagatgc
     2821 tattaatgaa gtaaagaaaa agattccaga caatataaat aaacagaaaa taagagatga
     2881 gtttactaaa atcaactatg attatgctaa aaaactgagt gatgagtgtt gttctaatgt
     2941 tgctaaaaaa tcaactgata gagaagaaaa agtagataaa atattaacat ctaaaatatt
     3001 tgggcttcca ataatgctct tattattagg tactatattg tggattacta tagaaggagc
     3061 taactatcca tccactttat tatctaattt attactcgga tttgagccaa gtatttcggg
     3121 tatattaaat agtattaatt gtccatcatg gttaaatgat atgttggttt taggattata
     3181 cagaacatta gcatgggtaa tctctgtaat gctacctcct atggcaatat tttttccatt
     3241 atttacccta cttgaggatt ttggatattt gccaagagtt gcatttaact tagaccacct
     3301 atttaaaaaa gcttgtgcac atggaaaaca gtgtttgact atgtgtatgg gatttggatg
     3361 taatgcagct ggagttattg gttgtaggat aattgactct ccaagggaaa gacttatagc
     3421 tatacttaca aacaactttg ttccttgtaa tggtcgtttt cctactttga tagctatatc
     3481 tacaatattt tttagctcag ttataaccaa ctctttcgta tcaagcgtag caactgcact
     3541 ttgcataact ttactgataa ttctaggtgt tataataaca ttgttggttt cttatactct
     3601 ttctaaaacg ctattaaaag gagttccgtc tacatttaca ttagaacttc ctccttatag
     3661 agttcctcaa ataggaagaa ctttatatac ctctataata gatagaacaa tatttgtttt
     3721 aggaagggct gtaatggtag ccatacctgc tggtgtcata acttggatat ttgcaaatat
     3781 atatatagga gatttaagta tattatcaca tgttgcaaac tttttagacc ctttagctaa
     3841 attaattggt cttgatggtt ttatattact tgcttttatt cttggattcc cagcaaatga
     3901 gatagttgta cctatacttt taatggctta cctagcaact ggctctatga tagaattaga
     3961 tagctttagt gctttaggtc aagttcttag agaacatgga tggacttatt tgacagcttt
     4021 aaatgttatg ctgttcagtt tactgcattg gccttgtgca acaacacttt taacaataaa
     4081 aaaagaaact ggtagtttaa aatggactgc cttaggattc ttaatgccta ctatacttgc
     4141 ttttgttgtt tgttttttaa caactacagt ttacaattta tttatataat tagtattaac
     4201 tgacagtaga catatattag agaaactatt taaaaattta tatactttat tataaagcat
     4261 agagatatat tcaatgataa gtaaaattac ttaacatttg aatatatctc tattttaatt
     4321 cttatctgga tacttggatt gaaatttcag tcatataatt atcttcatta ctttcacaaa
     4381 aaaagtcaag tatactctcc tcatacgaat ttccaataat tttatattca ttttcatata
     4441 tatatttttt caatttttcg taagatttat atgaatcctc atattttcca atatgtttta
     4501 tgcaagcata cattccttct ggctttacat atagcttttt atcctttatt tttttatcta
     4561 tcttcaaata aaaacttgat atacttaaat agtccccatt ttttatattt tcattactta
     4621 ctattactcc caactcatat ccttgataca aatttttcga tttacaataa ttggtaaaat
     4681 ctattaattc catcataaaa tcactattac tcatattact caaatcgctt gatagaacta
     4741 aatattcttc aaactttttg gatatataaa tttcttcact attcatttta gaaattcctt
     4801 caactgttat atttattcta ttttgcatct tagaacttat ctttcttagt ttttttattt
     4861 tttctcttat ctttttttct tcttctttaa gcattttcac acaaagctgg ggagttctat
     4921 tttgtatata ctcttttatt tcctctaaac tcattcctat ttctcttaat gattgtaata
     4981 tatcaagtgt atcatattga taaactgagt aatatctata attattttta tctttatatt
     5041 caggagaaaa aatacctatt ttgtcataga aaattaatgt ctgcttagat ataccacaca
     5101 atttagcaaa ttcacctgtt gtaaaatact gtttttccaa gggttataca ctccttttct
     5161 ttttgttgtc tatatagtta ctatattctt tattatttac taaatcaagt agttattatt
     5221 ttagaaaatc aatatggttt tgattttaaa gcttaaatat cagttagatg tcagttataa
     5281 atattttacc cattcccatc ataaaaaaga tatcccctct taacttttgg atggaatatt
     5341 atatttatat ttgttagagt gaaaattgta ataggtgcta caataagata tttaattaaa
     5401 cataaagtta attattgtaa taaaaaatct ttagataaat tagagttaaa gtatggaaat
     5461 atagatagag aaaaagctgt taaactagaa atattttatc agtatttaat agaacttgaa
     5521 tatatagaaa tgagtttatt aataagaaga ttagatactg caataacatc attagcatta
     5581 gtatctatta taacaatagc atcatattat ttaatcagaa aaaatgtgta attatgtaag
     5641 ctatttataa taaaacactg ataaaattaa taataaaaaa ggtgataaga tttctatatt
     5701 accttttgac ttttataata tagtgtaatt ttattatatt tttgtatgct aatataacta
     5761 tataatcata aaataacacc ttaaaaaaaa ttataaaaat ttacattcca tatagttaat
     5821 ataaaacact tgtaaaatta aaagatgaag ctagtccaac tttgagattt acattccata
     5881 tagttaatat aaaacaccac atcaaagtta tctttaatag aagttatctc ctatttacat
     5941 tccatatagt taatataaaa ctttctcctg caagtaacat tcttcttaaa agttctttat
     6001 ttacattcca tatagttaat ataaaacgtt agctccatat gtaaaaaaag cattagaaaa
     6061 agaatttaca ttccatatag ttaatataaa accaactgaa tttatagtta aaacttttaa
     6121 actttcagca tttacattcc atatagttaa tataaaacgg tcattataat ttatacttcc
     6181 aatcaaacga tttaaattta cattccatat agttaatata aaactcttaa aaaatagtga
     6241 agcacttggc tacgaaaaaa atttacattc catatagtta atataaaacc ccaaaataaa
     6301 cttagtattt tcaatactta cacgtataca actctcttaa atttgcagtg aacgagcaat
     6361 agtgcaattg ataacgctta tcatacactc tcaacgcctt atatttcaac tgttacgcca
     6421 aattttttca caaatatcgc tcactgcaaa attcctataa ttttattata tcataaatat
     6481 attacttttg aatattgata ccaatttata ccataataaa agcaaaaaaa cgtaatctat
     6541 ctacattaga tttaaattct ataacttaaa gttttttaca taatatttat gcttgtttaa
     6601 tcaatctaat atatcattct tgtatattgc ttttaatagt cttttttctt aaataaatat
     6661 ctatctgaac aagcatagtt taattaaaaa cttttgtatt cccttaaaac tgtatctacc
     6721 tcttattatt ttatttatga tatattattt ttaatctaaa aatagatatc tatttttatt
     6781 atggttatcc ctttttaaaa tactttaatc atatactatt cccaatttaa catccattga
     6841 aatgctattt tcttatattg caatttataa ttgatatatc ttcaatataa taatttaatg
     6901 tatttttgat tttaaagctt ttcagtgata ttattgataa attagaatta attatctagc
     6961 tttaaaaggt caaagatttc tgttgctgtc tgcggttttc caaataaata tccttgtatg
     7021 taatctggct ttatttgttt aactaaatca tactcttctt gtgtttctac accttctaaa
     7081 catactttta ttcctacatc atgacaaatt gcaacaataa aatgtataaa tgtagcatca
     7141 aacttactct ttaatatatc tttaacaaat actctatcaa ttttcactat atctattgga
     7201 gaaaatttta aaacttctag agacgagtat cccgtaccaa aatcatccat agcaatataa
     7261 attcctaaat cttgtaaagc cttaaattta aactgtaaaa tactcatatt ttgtactgta
     7321 tgactttctg tcaattctaa aacaagattt tgataaggaa aattttcatc atctataatt
     7381 tttactatat cttcaataaa tgtgtcttct aatatttgaa cagctgaaac gtttacactc
     7441 actttaaaaa aaggatagta atcaatccat ttcctaaaag tacgtaatgc cattcgaagc
     7501 acccaagccc ctacaatatt tatcatatca ttttcttcta atattggaat aaattccaaa
     7561 ggagaaattg ctttgcattt gtcattagtc caccttaaaa gaacttctac acctattatt
     7621 ttatgacttt ctgtatctac ttgtggctgg aatcgaagac taaaccccct aaaatcatca
     7681 ttaattgatg cctttaaatc ccgcatcatt tctaatgaat acattttatt ttttaatatt
     7741 tcttgggaga aaaaaacaag cctgttttta ccatgctctt tcgcatattg taaagagtaa
     7801 ctagcacact tataaagttc ttttactgta tctccatgtt ttggatatat tacacaacca
     7861 gcagaaattg ttatatttaa tccatatttt ctccatagtt gaaggtgtat aatcatattc
     7921 tgtatttgat tatatagagt tagaatttca ttttcttcaa cattatcaac aagaatgccc
     7981 atttcgtccc catcaagttt ataaagttca gcattatctg gtaaaataga ttgtatagac
     8041 tgtgctagtg tttttattat attatctcca aaatccctat catacatctc attaacattt
     8101 ttaaaatcgt caatatctaa taatactatg cataattttt caatcatagg atttgaaatt
     8161 ttatcttcaa atacactcat aaattcatga taattcagta actgtgtcaa tgaatcaatc
     8221 ttattttgtt tacctaattg cgtcattatt cctgcaaaaa tacttggttc tccaaattca
     8281 tcacgcatta gttgacctct acatcttaac catatatatt ctccactacg atttttagcc
     8341 ctaaattcca ctgtatgata atccatctgg tttttaccta tttcagtgtt tgacttataa
     8401 aaacgattcc agtcttctgg atgtacaatt tttttccagt aaacaagcgg atttttaaca
     8461 atttctcctg gtaagtcaaa taattcaact tgtgatggtg aatatcgaaa tacgcctgtt
     8521 ttcatattgc aaatgtatat atattcatct gtacctctaa tcaaagcatc gtaaagcaat
     8581 gtagaattat aatcaaaatt gtctcttgaa aaattaaaaa ctcctttttc ttctttatta
     8641 tttgatattt catgaaattc attaatctta attggtttat ttaagcttgt tgagtttgca
     8701 atatctattg attgatgcat atgaacaact gtgccatctt gccatgtaat taaactatca
     8761 taattttcaa aaacacgatt taacttatta catttttcat accactttat taatactcct
     8821 tttttatcat tctttaataa ttctaaaact ttacaaaaac tacaagtaga atttttttca
     8881 ggatataata cttgccaaca aacttttcct tctggatcta aaatattata ttcttctttc
     8941 atttttttgt tcataaaaat aatttcatta gtatgaatat ttgtaacata tatattaatc
     9001 tgtagttgat ttaatataac ttcaaaatta tgtttgttca tatacttatc tcctccatag
     9061 ttactgctct ttataacaac attgttaaaa ttattctttt tagaagattt acttataact
     9121 tatacatata cactaaatac cttaatttaa ttagaaagtt tattacttca tattttaaat
     9181 tataattgat aaaaaaaaat acagcaatat agtattacca atttaaaaaa aattaatatt
     9241 acataataca tctctctctt atattaaatt ttaacttggt actaatcttg acaaattgtc
     9301 cacaaaaata aattatagat tttattattg ttttaaattt ttataaaaaa tcagtattat
     9361 tgatactatt gaagttaaaa agcattaatt tctaaaaaca tcataatttt tctaatagac
     9421 ttaatcactt ttaatatata tagtaaaaca aaaaacttat attttataaa tatcataatt
     9481 tttttactga tattttatta tataaatttt atagaattaa ttgtcaattt tttatgaaaa
     9541 tggtaagata tttatggatg gtatcttgtt ataagaacta tactttaaat gtttttaatt
     9601 attttgtctt atgtaactca tatcctcagg aatgagatta gaaattaaga tgttgtatca
     9661 caaagtggag gtgatactat taaaataggc tttattggtt ctggaaaagt tggaacatct
     9721 ttggcaaatt attttttatc aaaaggttat tgtgtttcag gcttttatgg aaggaatcaa
     9781 ttatctttat tagaatcaac aaatttaaca aaaacaaaga tttacagcaa tttgaaagat
     9841 ataatttatg aaaacgatat attatttatt acaacatcag atgattctat tgaaattata
     9901 gacaaaaaac tttccaaatt taatttaaaa cataaataca tttgccacac aagtggctct
     9961 ttaaaatcta gtatattgtt ctgttcaaaa aaagcaggtg cattaattta ttctatccat
    10021 cctatatttg cattttccaa taaaaatacc aacataaaaa aaatgaaaga catttgtttt
    10081 tccatagaag gtgataatga acaagatgat ttagttattc aaaatttcat agatgatttg
    10141 ggaaaccaat tttttataag agataaggat acttcatcca cttatcattt agctaatgtt
    10201 ttagtttcaa atttagtatt atctttacta gatataggag taagttactt tattaaatta
    10261 ggtttatctg aagaggaatc tttaaaagct atcagtcctt tagtaaaaaa aaacatagaa
    10321 aatatacttg ataatggctt tactaaagct cttactggtc ctgtttctcg tggagatata
    10381 acaccaataa ggaaacatct atctgtattg gataaaaaag atgaggatat ttacaaaata
    10441 ctatccttaa acctactaaa aataattgcc ttaggaaatg acaattcatt aaaaggtata
    10501 aaaaatatta ctatagaaaa tgcaatcgaa aatttaataa gtaaatctga aaaatacaaa
    10561 gaaatttaca aacttttagg aggaaaaaac gatgaaaaat accatacaga cttttaagaa
    10621 tgctaaatat gaaggaaaaa aactatctat gcttactgct tatgactact caattgcaaa
    10681 aataatggat gagtgtgata taaatggtat tttaatagga gattcattag gaatggtcat
    10741 aaaaggtgaa gaaaacactc tatctgttac catagacgaa attatatatc atacaaaagc
    10801 tgtaaaaaat ggtgtaaaaa atgccttaat cgtaagtgat atgccttttc tatcttacca
    10861 tgtttctata gaagatgcag ttaaaaacgc tggtagactt ataaaggaag gaggagctca
    10921 tgctgtaaag ttagaaggtg gctcaaacgt tataaagcaa atagaaagca ttgtaaatgc
    10981 tcaaatacct gtaatggggc atttaggatt aactcctcaa tcagtaaatt catttggtgg
    11041 gtttaaggtt caaggaaata ccagtgaaac agcaagacaa cttattgaag atgctaaatt
    11101 aatagaaaaa gcaggtgcat tctctattgt ccttgaagga gtgcctacga aaatagctga
    11161 gatggtaact aattctattt caattcctac aataggtata ggggcaggaa ttaactgtga
    11221 tggacaaatt ttagtttatc aagatatgtt gggaatgttt ggagattttg ttcctaagtt
    11281 tgtaaagcag tatgcaaata taggagatat aatgaaagat tcaattaaaa attacatcct
    11341 agaagttaat acaggtgcat ttccacaaga aaaacacagc ttttcaataa atgaatctga
    11401 gttagaaaaa ttatactaat attaaggatg gtacaaaatg cttgtaaaag aaattaaatt
    11461 actaagaaat ataatcaaag attggagaaa acatggctat tctataggcc ttgttacaac
    11521 aatgggattt ttacatgaag gacaccaaag tcttataaaa aaagctgtaa aagaaaatga
    11581 taaagtagta gtgagtgtat ttgtaaatcc aactcaattt ggaccaaatg aagattttaa
    11641 tagctatcca agagatatag ataaagattt taaatattgt atggatagtg gagcaacagt
    11701 agtctttaac ccatcaccag aagaaatgta tttaaaaggt aattgtacta ccattaatgt
    11761 ttctggactt acagattttc tttgtggagc taaaagacct gttcattttg gaggtgtctg
    11821 cctagttgtt tcaaagtttc taaatatagt aacacctgat aaagcttatt ttggagagaa
    11881 ggatgcccaa caattagctg taattaaaag aatggtcaag gatttaaaca tagatacaga
    11941 aataataggc tgtcctataa ttcgtgaaaa tgatggatta gcaaaaagtt cgagaaatac
    12001 atatctctct gaggaagaac gaaaatctgc tctaatatta aataagagct taagtttagc
    12061 aaaagaagaa ttagtaaaag gcaatctaaa tccagagaat ataaaagaat taataacagc
    12121 taaaataaat tctgaacact tagcaaaaat agactatgtt gaaattgtag atagtgagac
    12181 attacaacct gttaagcaaa tagaacattc tatacttgtg gcaattgctg tgtttatagg
    12241 aaaaaccaga ctaattgata attttacatt tgagttaaat atatagtcta taattttata
    12301 taatatatct aaaaaataat agaataccta aaaagtaata gaatacctaa aaagtaataa
    12361 attacctaaa aaataataaa ttgcttagaa aataatagat tacaataact aaaattttaa
    12421 aattaaattc cttaaaagta tgattaatta taattatata agtatattct tattacactt
    12481 atattaattt atattaaata tctactaact caaatattca taattaaact ttaaactgga
    12541 taaattttct tttaagaatg aaagtttatc cagttttgcg attataataa ccagttttac
    12601 atatccactt gtaaaatata tctaagcaat atatcatact atatagattt atgagtgaaa
    12661 taatatctgc tatttactga atatttttat ttttcgtttt ttagggggat tagttgtgtt
    12721 taaagataag attgatgaat gtgttcatat aatgactgca tatattgcta gtttaaaaga
    12781 atactattca ttcatagaaa ctcaaattgg tgattttatt aaaaaatatg gagaagatgt
    12841 tgtagagtta tgtctccata gagtgatgat tctactatgc gaatgtggtt tggcataaaa
    12901 ttaaatttat taaaatgggt aagaagaatc ctatataata ttcttcttac ccattttaat
    12961 atatcaattt catagcttat cgtttaaaat attttacatc cattactaga caactttaca
    13021 ttctcttttt aattctagta tatttaaatc attttagttg ctccttattt attcttatta
    13081 cttgttttat tttataaata aaataattct ttttaataat gttttttgtt gatattccta
    13141 tcacacatcc aattatttat cattgtcttg aaattgtttg catacttaat ttaatccaat
    13201 tgaaacaccc caacatagaa tcccctgtaa aatagaagtt ggattaaatc ctaacatcta
    13261 tacagaaaat actactccaa gcacaagcaa tataatggaa tatatttatc acttaatttt
    13321 ttatactttt taaatcccat acctaaaaca ttaagtgcag ctattagaat taataattgt
    13381 tgtggtataa aattatgctt gcttcatatt tattcatttt ttgctataaa agttcttgtt
    13441 ttacaaaaaa catttgtttt gtatcaaaat aatacttata aattacataa ttctcataaa
    13501 aatttctagt attacttgta ctcttaataa ataatagcca acaagtacct taaatcatta
    13561 tatctatatc taataagaaa cacagtaaga taattttttc gcccataact taataaaaac
    13621 tgttgatatt agtagcaatt gaatttagtt aatttattta taatatacat aaatttaaat
    13681 attacgaata aagtattata aataggatac tttctaaagg gaggaaatta tgatagataa
    13741 tcaaaaatat gttattttat cactagaatt acatttattt ttttcaagaa ttatgaaaga
    13801 gcatgctctt tttttagaag caggattcac aaataaaaat tataatcttg ctatggaagc
    13861 tgaccactat aaaaagcaat ttgaagattt attatcatac actgttagtg ctagtaatgg
    13921 tataattaga cctgatatat tatattcaga agaacttgta actactctca catcagttgc
    13981 agaacaaaaa acagaagagt ttacagggat agaaataaac aaaaacatca ctacaagaga
    14041 attaaatcta caaagtggtg taaacccaca agttggtcaa gatttagtga actatgtagc
    14101 tcaacttaac tctgatgcaa taagattact tgatgggctt attaatttta aagaaagagt
    14161 cttagatggt gtactatcat gtactatatt tacatcaaac taccctctac ttcttgaaca
    14221 tataatacat gaagcaaatt tatatcgttc ttatgtagtt gaccttgaaa ataaaataga
    14281 tattgagtca aaaaacgcta aagaaataga attattctgg gaccatatta tgatggaaca
    14341 tgctctgttt atgagaggat tactagaccc ctcagaaggt gaactaataa atacttcaaa
    14401 tgattttgct ataaaattta atgaattaat tgaaaaaaca aacgaaatga ctgattctaa
    14461 tatcaagaac attacagaag aaactctaaa tgaaactgtt gagtttaaag attttaaaga
    14521 agcaggagca tcaggaatag aacagtgtaa gataaaatct ataatattac cacttttagc
    14581 agaccatgtt ttaagagagg caaatcatta tattagaata ttggagagtt ataaaaacat
    14641 gtaattaaaa attactttta aaataatctt tgcatattta gataaaataa aggtacagtt
    14701 aactgtacct ttatttttta taaacttata aaatttaaac aagttattca taaaataaaa
    14761 taataatcat ctataaccca caactttatt ttatattaaa taaaaagtat tagtggttat
    14821 gttttataag tatttcttta ttcagttatt tattaaaaac taatagtaaa tcttatacta
    14881 atcgcatatt tatgttataa ctatattgta actattttcg taatcttagg aaaagattac
    14941 ttataaaatt aaccttatac aataatatat cgtataatct taattagcat aatagttaca
    15001 atattaaaat ttaaatatgg gagtgatttt agtatgataa gacaaacacc attagagaaa
    15061 cgatatgaca agctaggtcc tcatattgta acagcactca aaaaacgtta ttttgatgct
    15121 tattactgta aaactagaaa tgaagctttg caacaaattt tagatttaat tcctcaaaat
    15181 catgtagtat catggggagg atctgaaaca cttaaagaaa tgggcgtaca aactgctgtt
    15241 agagagaaag gatttaaagt tattgataga gatttagcaa aaactccaga agaaagaact
    15301 gaaattatgc gtcaagcact tctatgcgat acttttttga tgagtagcaa tgcaattagt
    15361 gaagatggac aactctttaa tattgatgga aatggcaatc gtgtagctgc aatgatattt
    15421 ggtccaaaaa atgttatagt tgtagctggt atgaataaaa ttgtaaaaaa cattgatgat
    15481 gcaatacaaa gaactagaac agttgcatct ccagcagttg ttcagcgttt tcaagatgtc
    15541 aatacaccat gctatattaa tggaagttgt atagattgca caagtcctga aagcatttgt
    15601 gcatacatgg ttactacaag aatcagtcgt ccagcaaaaa aaataaaggt aatattggta
    15661 ggagaaaatc ttggtctata aaatatacat acatgtaaat agctctataa ataataaaat
    15721 catatgaaat aacttatttc taaaagtggt agaataaaag cttctacaag actttcattc
    15781 taccactttt atattctttt aatttgtgtt tctatattat taattgctcc gattttcata
    15841 tatattacta actattttta ttttcataat ttttttgttc ttcatcaagc tgtttataat
    15901 aatctttatt atagtcagct agataatcta taccacctcc tgtaatcaca tttaataatt
    15961 tcattgcaga ttcttgatta actttacaaa cataattatc accagattta acaagctcta
    16021 atgtaatatt aaattctttt ttctttgcag aatcaatatc tttcatagtt ggatttggca
    16081 tcatttctcc tagtttagga ggattctttt ttaaaataga aaccatatca gtattgttaa
    16141 tttttaaatc tacagtcact ttatccttat tattctttat atctgaaatt tcccaagtca
    16201 tatttttaga atacatttta aatccttctt tatcttcgta tataagatta cctaaccctg
    16261 attttgggaa atacttatca acagtatctg catcttgtgt ttttattgaa ttaaacatac
    16321 tatccacttc attttttatc ttattatcat tagtacaacc agctaacacc agtactgata
    16381 ttgcaaacat tataatacat ataataaatt tcttcattaa attatatccc cctatacttt
    16441 tttgcacaaa tattattctc ttttttatct tttgaaaaag ttgtagtaat tgcattttgt
    16501 ggacaaactt ttatacattc accacaccta atacagtctg gactatttac atctttatat
    16561 attggtatat caagcttaca ttttgtttga catgctgtac aatttgtaca tttatcttta
    16621 tcaattttaa atctatatat tgaaatagga ttaaaaaaac catggattgc tcctaaaggg
    16681 catatatact tgcaaaatgg cctataagta atgattgaca tcaataatat caatagcaaa
    16741 atacttaact tccagctaaa taaaaatcct attgattctc ttaaattctc atttcctaaa
    16801 ataagtggta ttcctcccaa aagtgttcct gaagggcaaa tccatttaca aaaccaagga
    16861 tttccacccc ctgctacatt tacaatagca actggcaaaa taattacaaa cattattaag
    16921 actatatact ttagatattt tagatattta tcaccataaa ctttttttat ctttactgga
    16981 aagggtactt tatgtagtaa atcttgtaca agtccaaagg gacaaagcca accacaaaca
    17041 aatctgccaa aaaaagcacc aaatgccatt aaaaaaccaa caacataaaa tgagaattta
    17101 tactctctgc ttgataaaac tgcttgtaat gaacctattg gacatgctcc aagtgcacct
    17161 ggacatgaat aacaattcaa tccaggtaca cataattttt tactttctcc tccatatata
    17221 gttccttcta aaaatccatt tacatatcca tttgttaatg ctgtaaatgc aacttgagta
    17281 ataagtctta tattattatt tactttagat aataactcca ttcctcttct cctaaccaat
    17341 accaatacac tcaagacaaa tcataattgc tttagttaat actgtatttg tttctccacg
    17401 atatgtacct aatgcaataa atataatact aattgcaata atagaaatgc cttttttatt
    17461 atctttaata aaattactca ttggctttta ccatttttag tctttcatct ataatttttt
    17521 tccaatcttc cttactattt gcacctacaa ccttctcacc cacaaagttt cctttcttgt
    17581 ctacaaaata agttgttgga aactccataa ctcttttaaa atcagttttt ttaagttctt
    17641 cagatacagt caattgttgg taagttgcat ctgacttttt cataatgtct ttaactaaat
    17701 ttttctcttc atttgaaaga ccagttctca tatcagtttt atcaacttct acaactaatc
    17761 cctttatagc tacattggaa ttattactct catattcttt tgataatgct tcaagctctg
    17821 gcatttcacc cacacatggt ccacaccaag tagtccagac attaataaga gtaagttctt
    17881 tatttgaaaa tatctctttt gttaccttat ttccatcaac atcctccgta tctaaattac
    17941 taaaccattc actattattc ttgttttttt ctactggctt agattctttt ttgctacatc
    18001 cagaaatcat acctaaaaca caaatacatc ctaaaactac tatgaaaaac cttttcatat
    18061 ctcacactcc tagaatttat atagtatata aaatcgctgc ttgaaataaa aataacttat
    18121 cttctatatt aaagtaatta tatttaataa ataagagcta atttaaaaaa agatatacag
    18181 ttaaaatcta tttcttttca gacaaaatct tatatatata taatagcttg taaatcttaa
    18241 gattatatac atatatttct aaagattttc ttaagaaaca gttagatttt actaatataa
    18301 gtttattaat tagcattata ctaatttatc ttaatatgat tttttgttga ttttaagctg
    18361 tttatcgtat aaataaaatt tttcttgact atatagttac tatatagctt attatatata
    18421 gagagtaata aaaggaggta aaatttatgg aaaatttatt tacaagaaaa ttcactactt
    18481 ttgaatttct aaaatttgtt tctcctgcaa ttatatccat gatatttata tctttgtaca
    18541 caataataga tggtatcttt gtatcgacat tagttggttc tgatgctctt gctagtataa
    18601 atattgtact acctataatt aaccttgttt gtggatttgg aataatgatg gcaactggtg
    18661 gaggagctat cgtttctata cgtatgggtg aaaatagaca ggatgaagcc aactctacat
    18721 tttcttttat agttttgttt tcattgattg ttgggatttt attcacagta atctcatatt
    18781 tctttatcaa agaaatatct attttgcttg gtgcaacaga taagttatta ccatattgta
    18841 taacttatgg taaagttatg attttatgta caccatttta tattttaaaa tttatatttg
    18901 agtactttgc aagaactgat ggaaattcta aatttagttt atttctatca gtcattggtg
    18961 gtgtaacaaa tataatattg gattatgtat ttattaaata ttttggaatg ggtcttttag
    19021 gagctgcagt tgcaactgct ataggtatta ttttaacttg tgttttaggt attatttact
    19081 tcttatctaa taaatctaca ctaaaattaa gaaaaccaaa aaccgatttt agacttataa
    19141 gagatactat gataaacggt tcttctgaaa tggttacaga attatctaca ggaattacaa
    19201 cattcttatt taatgtagta gctttaaaat tagcaggaga aaatggactt gctgctctta
    19261 ctatagtatt gtatgctcat tttttaatga catcagtcta tctaggattc gctgctggag
    19321 tgtctccatt aataagctat aattttggtg ctgaaaacag tgataaatta aaagaaacat
    19381 ttaaacattc tctaaaattt atatttattt cttctctttt agtgtttatt attgctttag
    19441 tatttgcacc atttattgtt agggtctttg taaatccaga taacacagta tttaaactag
    19501 ccttacaagg attaaaaata tttgcatttg cttttttgtt tgttggtata aatatatttg
    19561 catcaggatt ttttacagca tttcacaatg gaaaaatttc agctattata tcttttagtc
    19621 gtgcctttgt ttttataatc ataggaatca taattcttcc tcctatgtta aacatgactg
    19681 gattatggct tacagttcca tttgctgagg ttataaccat atttatatct attctattta
    19741 taaaaaaata taaaggtaga tataagtatt aaaaattaat acttaaaatc aacatttatt
    19801 aaaactaata aacctgcttt aaaatacaaa tttacaaatt tgatttttaa gcaggtttta
    19861 ttttagttat aataagtcat ttgcatactc tttaattggt gcttttctaa gtaataaaat
    19921 tataaatatt atgcaaataa ctataattcc tacctttgtt ccaaataaaa taaaaggatt
    19981 ctgaaatgaa taaataattt tttctaaata caatagcatt ttatgcatat ttttagttgc
    20041 aaaattaact atataaatta ccattaatat tggtaatcct atcaatatag taatactagt
    20101 aacttttcta actctatatg taaaagctcc aagtaacact cctataaaac ctatacatat
    20161 ttcatttata catatttgta caaaataatt taaataagtt agtggcatac ttgatgttga
    20221 aggatctatc agtgctgata tagatgtcca aatcatatct ctctttagta aaactgatag
    20281 ttctatacta taacctgtta taatttctat aaataacttc gataaaaaaa tcaaaactat
    20341 acctaaaata gaaatcacaa agcttaaaat aattaaatct atgacaatag cttttataca
    20401 actttttcta tctgctctta tacttaaagc tcctgaaaag tctttatttg ctatatttac
    20461 tccatacaca aatagcagta tagttatata tgttaaaata ggattcacaa tcgtggataa
    20521 attagtttta gatgcttctc caaaaatctt gaggataaac actggcaaaa tattcataac
    20581 aattcccaca atcataaata taattatata caatttagta tttttattaa aaaattttat
    20641 atatggcttt aattcattca attacaattc ctccttctta gtcatgttaa caaacatatc
    20701 ttgtagccct atgtatccta catcaatatt tgaattttta agtatttttt catcattttc
    20761 actaaaatca ccatagtaaa aataagcgac tgcatttcca aatgcctttt taggttttat
    20821 attttttata cattctaatt tttctaactc ttctttattt ccagtaatat aatgagcttt
    20881 ttgttttatg acatcaattt cttcatctat aataattttt ccctcattta atattattac
    20941 atgctccagt aaatcatcta tttcatcaat caaatgagtt gatattatta ttgtccttgg
    21001 attttttata taactatcta aaattatgtt ataaaactct tgtctattta ctgcatccaa
    21061 accaattgtt ggctcatcaa atatggtgat agctgaatta gaacaaatac ctattatatt
    21121 tgaaagtata gttttcatac ctcttgacag ttgtttatac ttttttttag tatttaaatc
    21181 aaaatatttg cataattctt cttctaagcc tttatcataa cttttataaa aactagaaga
    21241 aaattcaaat atctgaccta ctttaaaatc tggactataa aattcttttt ccctgacgat
    21301 acaaacttct tccaaaactt ttatatcttc ttttgggttc tttcctaata tttctatctc
    21361 accttcattt tgaattaact gatttaccat tatatttaat aatgttgttt ttccagctcc
    21421 attcttacct aataaaccat atatcttatt tttttcaaag tttagagaca tattattaaa
    21481 aatattttgc ttcttaaaac tgtgagataa attttctatc ttaatagaac tactcatata
    21541 ttaatcctcc ttgaaattaa ttataatatc aaccaattct tctctattta tttctaattt
    21601 gttagcttct tgaagtaggt tttttacaaa attatgttta aaattttctt ttctagcttc
    21661 ttttataata gtttttgcac catctgatac aaacatccct atccccctct tcttatataa
    21721 aatattttta tcaaccaata tatttatacc ttttaatact gtagctggat taattttata
    21781 aagctttgaa agctctgttg ttgatggaac ctgttcttct tcttttattc catttgatag
    21841 tatctcatct tcaattgctc gtgctatctg aataaatata ggttcttcat tatttaatac
    21901 taatttcata ggtgcctcct ttctccatta gttagttact caactaatta actatataac
    21961 ttataatatc ttgaaaaata ttttctgtca atactaaata aaaaacagag ggaaaattcc
    22021 ctctgtttgt tgttctttat atttataatt aaattttaat ttttgtaatc ctatccataa
    22081 gttaaaaact ttaataatct gttgtatctt cattttttaa tattttacat attgagctag
    22141 ttcctaacct ttctgctcct gcctctataa atttttctgc atcagaaatt gatttaactc
    22201 cccctgcagc ttttattttt acattttttc caacatgctc tttcataagc tttatatctt
    22261 ctaaagtagc tccacctgtt cccattccag tagatgtttt tataaaatct gaacctgaca
    22321 ttgttactat ttcacacatt tttatttttt catcttcatc taaataacat gtctctataa
    22381 tgacttttaa tactttatct cctatagctt tttttatttc ttttatttct tttcctatat
    22441 tatcataatc tttatttttt atatctgaaa tgtttacaac catatcaact tcatctgctc
    22501 cattttcaat agcatctttt gcttcaaata cttttgtagc agtagtttga tatcccaaag
    22561 ggaaccctat cactgtacat atctttattt taccttttag atattctgat gctcttttca
    22621 catatgatgg tggtatgcaa actgatgcta cactcatatc tacagcttca tcacataaaa
    22681 ttttaatatc ttcccatgta gttgtagctt ttaaaatagt atggtctact gtttttaata
    22741 tatgtttcat aaaatcctcc taaatttata attaatataa ttagattact tttttaaatg
    22801 caattttctt tttgcaatat tcagtatcat atcaacctct tcaactttaa aaatagtcat
    22861 tagtagtgta taaattccac ctccaactat aactgaaata gctagtgata caaactcatt
    22921 aaatgttcct actcccaaaa ttccaaatat aaatttatac gtaaagtatg ataaaacacc
    22981 cataattaaa gatgcaacta ccgatttcaa agttgctttt attattttat cttgtccaaa
    23041 atatccaact tttcttttta ggttcaagaa gagtaacaat atacatgcaa ttgatgatga
    23101 actagttgca agagcaagac caccatgagc cattggtttt attaaaacta agtccaatac
    23161 tatatttacc cctacagata taatcccatt taccataggt gtttttgtgt cttgtaatga
    23221 ataaaaaacc tttcccaaaa tatctcttaa tccaaatgca gtcataccaa ctgcataaaa
    23281 aattaaggca gttgctgtca tttgggttgc cctcgcatca aaagcacctc tctcaaaaat
    23341 gattctaaca acaggagttg caaaaaaaat agaagcaact gaaataggaa tcatagaaat
    23401 tattatcata tttatactag atttaactga actagtaaac ttcttttgat tattttctgc
    23461 acttaactta gataacatag gatacataac agatactatt gaagctgtaa atgttcctgt
    23521 aacaaaacca tttaatctat ctgcataagt aagtgctgga atgcttcctt taactaatgt
    23581 tgaggctaaa gttgtatcta ccaatgagtt cacttgatta acagcaacac ctataaatac
    23641 tggtgaaagt aatgctagta atttaataag gctatcatct ttaaagttta agtagtataa
    23701 gtatttgtag ttcgtttttt taacaaaaaa catgtaaaag agcagttgaa ctaccatagc
    23761 aaccacagca ccaattggaa gtatgtatgg tccaaatatg gtacttaaca ttattgaaat
    23821 aattattact atattatagg gtatacttcc aaatccaact acaataaagt tctctttaat
    23881 ttgtaaaaat gctgacataa cacttgtaat acctatgaat attattcctg ttctgaacct
    23941 cccatgacta aagtcacagg gttcctgctt catagaattt tgcatactaa aatatgtctt
    24001 actaattctc cacaggctat ccccgtagtt cctacggttc ttacataagt tatttatttt
    24061 gaatcgttat taatcttaat ccttctttta atatatttct acttgcattt atatctctat
    24121 cgtgagtttc attacaacta ggacaaatcc attctcttat atttaagtcc ttcacttcct
    24181 cgtttttata cccacattta ttacatattt gtgaacttgc aaagaattta cctaccttta
    24241 caatttgtct accatgccaa ttagccttgt attctaattg acgaataaac tctgaccatg
    24301 atacatcaga aattaagcga gatagtttac gatttctaat catattcttt acttgtaaat
    24361 cttctatgca aataatatcg ttctctttta ttaatttagt agatagtttt tgtagaaaat
    24421 catttctttg attagctata tgttcttgaa gtcttgcaac ttttaatctt gttttatttc
    24481 tatttaaact accgattgtt tttctcgata attctctttg taatttagca agtttgttta
    24541 atgacttctt aagatactta ggattttcta taaattctcc acaact
//
LOCUS       DBGJFF010000055        23895 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.056, whole genome shotgun sequence.
ACCESSION   DBGJFF010000055 DBGJFF010000000
VERSION     DBGJFF010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23895
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.056"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            59..559
                     /locus_tag="ACOIAF_002152"
     CDS             59..559
                     /locus_tag="ACOIAF_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HGZ2669161.1"
                     /translation="MIEGIKFIKAEEKYIYSYWQTFDKIAKERKYLAMDEAFPFEETV
                     EFIKNIINKNLPQLFIIDLESDNCIGWCDVLPKTEKVGYLGMGILKEYREKGIGSSLL
                     KQIIDLSKEYGYEKIELDVFKSNSRAIHVYKSLGFVEVNTISSGFTWNDRPVKEEVIQ
                     MELTLI"
     gene            complement(809..1588)
                     /locus_tag="ACOIAF_002153"
     CDS             complement(809..1588)
                     /locus_tag="ACOIAF_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669162.1"
                     /translation="MNKNILNNSFPEVPESFHNSLSKTLNSLPEREENYKMINNKTHK
                     LPFRKGLVATLAITLILSTTALAVGHIYSLVGKSSTKATYTSIPTTEQVKKDFNFTPN
                     IVSEFSNGYKFKGAYTVNKKAFDKENNVLGKSKALSFEYKNGNDEISLDTANDVLGKS
                     SDNLKVVDTYKDINISYEAFAQKFVPEGYKMTEQDKIDEKSGKYTFSVGPNNESDKVE
                     VNKFKFLTWKQDGVYYSFTVQDSNLSMNDLVKMAHEVIDAK"
     gene            complement(1585..2064)
                     /locus_tag="ACOIAF_002154"
     CDS             complement(1585..2064)
                     /locus_tag="ACOIAF_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HGZ2669163.1"
                     /translation="MDKTTFTNNILESEQTLYRVSKSILGNDQDCEDAINNAILKAYE
                     KLDSLKEEQYFKTWLIRIVINECNSLRRKRLKSLSFEDVFKNKKIDEKDDYSDLYTAI
                     QSLPKKIKIPIVLYYIEGYSVDEVKEILDIPQGTVKSRLSRGRRLLKTKLENTEVII"
     gene            2873..3574
                     /locus_tag="ACOIAF_002155"
     CDS             2873..3574
                     /locus_tag="ACOIAF_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HGZ2669164.1"
                     /translation="MKFQEMINSPGLWIVSSFLVIISVVQAIVFMKEALKEASNLGIE
                     RKGIKAAIRSASVTAIGPSLSPVITLLSLVAVIGAPTTWMRLCDVGAARTELGVISLT
                     SNLSGVEVGSAAFGAEAFSYALWGMALNNLGWLFVVFILGHRMRGIVEKMNMKYNPTW
                     VKKLLAGATVGLFAYLLSNQIKTFEIPKLTPAIISAIVMLVLTTLFKKNQRLQELSLG
                     IAMLIGMFGTQIILS"
     gene            3600..4256
                     /locus_tag="ACOIAF_002156"
     CDS             3600..4256
                     /locus_tag="ACOIAF_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669165.1"
                     /translation="MYDKKMIKIGRISIGLAIIANFIPAIYVGIRFGQMPMLSVIFQI
                     WGLVAATYGVSWIVQPIAYYPTLGAPGSYIGWLAGSVGDIRMPAASMAQKIAGVEAGT
                     HEGEVVGTIGTCCSIFVSASMITIFTIVGYKVIPLLPEFVTNSFNYILPALFGAIYVD
                     IARKDIRAGGCTIAVALAIMYFGEKIGIPGGLLTLFIVLGGILITRVFFVIDNNKTKE
                     "
     gene            4361..6001
                     /locus_tag="ACOIAF_002157"
     CDS             4361..6001
                     /locus_tag="ACOIAF_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HGZ2669166.1"
                     /translation="MFDVKIVNGIIVDGTGNSRYKSDVGIVGDKIMAIGDLSQKEAKE
                     TIDATGKFVSPGFIDFHTHSDLSLVYDKYTRSRIHTGVTTDVIANCGIGVAPIREEKK
                     QELIDYLGTRIIGTIPTKLELHWNTMQEYFDYLTENSPAVNVVAYVAQGPVRINEMGF
                     SKEPATLEQLKNMKLEVRKAMEAGCVALSSGLVYLPGAYTKKEEMAELCKELIPYNGY
                     YISHIRDEGDEEMEALDEAIYIAKTAGVPLHVSHLKVMGHKNFGTIDEVFKKLDEAEA
                     DGLEVTFDCYPYTAGMTSLGALLPPWAFEGGVENMVKRLEVQENRDRIIKELEEGIPG
                     WQCFYQLAGGWNGVVLASVMTEANKYVEGKTLMEVAKINGENPFDTFFRLLIEEKSKI
                     QVVVHTQGQEDTDKVVCHPKSCIGSDSMDLSTEGLLSLGKPHPRAFGTFGRIFSYYVR
                     EKGMLTFEEAVKKITYLSAKRLGIYKERGLLKENYFADIVVFDPDTIEDKATYSNPKQ
                     YTVGVEYVLVNGKIALAGGKQTDVCAGRVIKNPLSIAK"
     gene            6359..7681
                     /locus_tag="ACOIAF_002158"
     CDS             6359..7681
                     /locus_tag="ACOIAF_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669167.1"
                     /translation="MKILYVGPGMVIDKILAVSKKNFPNIEADFLRYDRYIEVPDLLE
                     KYRGRVDAVLFTGKSPFKFFEKRQKDFVLSDYIPRHETTLYRVLLEITYLLKFDISRL
                     SIGTYDKAMMKRLYKEIGVEFKDEHMFFAEQKYLEEDYIKFVLEFHKYNYQNNDVCCC
                     ITGLEEAYHIMKKDNIPVVLALPSEDVMVQSIKNIQMRYIAKKNNENQIIVLAIKLNM
                     PSEYSLMKEDEYAYLSQRIKILDKLYHFNSRIDGVLVEQSRSEFMIFTTKKILELETK
                     KYRDIYLLDMIREVSVINTYIGIGYGKTANEAKFNAYESIKMAQSQKNDAAYVVFENG
                     EIMGPLKPNPTVKEKNSFDERFYQIATETGLSANTVYKVFNKIINSGKTEFTSKELAT
                     ICGVSIRTMDRIILRLCDSGYCEVVSEQLMSKYGRPSRILRFKPFLLF"
     gene            7878..8885
                     /locus_tag="ACOIAF_002159"
     CDS             7878..8885
                     /locus_tag="ACOIAF_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HGZ2669168.1"
                     /translation="MGKYQDSKKVVRNYFEALENATGDEVENVLKQHMKSDYNWKGVY
                     PFREQEGTENVADVFWKPLKKSLSNMQRRQDIFIAGTNEIDNSEWVMSMGHFMGLFDK
                     DWLGIKHTRKMISLRYAEFNCVQDGKIAKTGLFVDIIGFMIQAGVNPLPPQTGSYFVY
                     PGPIDHNGLLFEDADEVEGVKTLALVNKMVDDLSELNESGAMGCPPEILEKSWAKDMI
                     WYGPGGIGASYTIPRYQEQHQLPFRNNLKGKTFNGHVCRFAEGNFACFFGWPNLTNVP
                     CGGFLGLPGGEVKADMQVVDVYYRKGDKLQENWVLIDIPYWLKQQGLDILERTKSIFN
                     A"
     gene            8923..10335
                     /locus_tag="ACOIAF_002160"
     CDS             8923..10335
                     /locus_tag="ACOIAF_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HGZ2669169.1"
                     /translation="MASTNKNNATSKEEEKEVSTSGLESCTVKENKAKMNQTNFSNIN
                     ENQTNENKTNTYKGNIDKGNIDKTNTNKSDINKVNIDTVDTDKLNTNTLTKKNKKVIF
                     KGEDNMSSTKIYYGDSLEVTPVGVMDYNDFSKQTKREQEIPGFDSKYRDFVDYIMKIT
                     HNIWEEKGIGVIYDTYHNNVTMHCGSSNLVGIKDVISNTLQTLHAFPDRRLIGQNVIW
                     SNFGADGFLSSHRVLSTATNLGDSNFGPATGRKINFRTVIDCATTNNRIHEEWLVRDN
                     LWIVTQLGLNPQEVAKGMAKASESKVLSLQSTYGICESMDGQFMPTKYQAKDDSVGEM
                     MLEMTSRIYNYKYINEVKKYYHDNAVVHFICDKDLNGYDEIQGMIVSLLASIPNGSYE
                     VERVTCNQREKNEGYDVSVRWRLRGINEGIGFFGQPSGKHIEVMGINHYHILQGKVKE
                     EWITFDGMDVLKQMYMGVEE"
     gene            10477..11742
                     /locus_tag="ACOIAF_002161"
     CDS             10477..11742
                     /locus_tag="ACOIAF_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGZ2669170.1"
                     /translation="MKTTVEIENDIILGKDYSNLKRGIAFFSIALIYFFYCYNFMVGT
                     FIKPTMIYALADGGFGFSLKQTEEIFAVMSFGTIPGTFIFGVISTKIGKKRTLISVAL
                     LIGLTTFIPMLSPTNIMLWKIARLCTGVVLGGVFGTAMPLVADMFPSKYRGKLAAILT
                     ALFSLAMIFGGKVYGVLGDANWQILMYTAIIPPIVGAILAIFFVPDDLEYTKELVKKG
                     KETGEKISYISMYKGKYLWIGLGTILLSGANFTAYSAFSNNATTYLVTGIGMSAAVAG
                     SIYSLQGIGQLVGYLFWGSIADKFGRKIPFIGMALAAVFVFIYTKLGGDNTTTFYMIS
                     VLLGVAVGYSGAWGAYYTELFPSKYRSLSSGMSFNGGRIISTFAIPAIAGTASGTLGM
                     MTIFYISIAVFVTGAIVWLFLPETLNNKK"
     gene            11784..13382
                     /locus_tag="ACOIAF_002162"
     CDS             11784..13382
                     /locus_tag="ACOIAF_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HGZ2669171.1"
                     /translation="MISSIDVCIIIGYLLLMLVIGYYSGKDNKNQEDYFLAGRSMPWI
                     PIALSVAATMISANGFIGGPGWAYVDGMYPVMVNITVPLAIFFALSITTPVIYKLKIT
                     SIYEYMGLRLGNYSRGLTIIQFFINSLIQASSMVYIPVLIIQMITGWSLYALVPVVVL
                     VSIIYTLLGGIKAVIWTDSIQMIVVISAVFIVIIVALKGMNLSFFDTLQIAQQSGKLN
                     TFDFSRDISVTNTFWATLIGGTVMWIRYFCFDQSQVQRVLTSKSLKCAKNSFVVSAFV
                     MNLVYYFMLLVGVILFVFYGGRTFETSNEIMITFILNELPVGAIGIIIAGVFAAAMSS
                     IDSILNSMTTVFTKDIYEFYFKKDNKESSLKVTMIITVVIGVVMTGFIIMGFGGSIKS
                     ILDLVGNYISYFAGPATGAFVLAMFTYKANDKGVSIGFIVGLVLGFFISNKYNVSWLW
                     NPAIGATITLIFGYIFSVILKKNHNADDIKKYTAFGMRNHLISQGMDKVDGVSIVPFS
                     LGKQELIVLAFFIIQYIILFLIQF"
     gene            13417..14439
                     /locus_tag="ACOIAF_002163"
     CDS             13417..14439
                     /locus_tag="ACOIAF_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGZ2669172.1"
                     /translation="MNQKKVHYNKSATSKDVARLAGVSQSSVSRAFGSANGKGVKPEV
                     RERIFRVANEIGYVPNLVARGMISGKTNVIGLIVGDSLGPFYNRIINLFVEKIQEIGK
                     QCLVFKVPRQQSIDSIISKVIQFQVEAVIITASAMNKVMAETCEENNIPVILFNRFIP
                     GIKISTVYVDPIEGGGMAAEYLLKKGHKNIGYIQFTRETSEEMEKKIGFYSKLRQSGI
                     HNLKEESANYDYDSGYEAGKRMLALSNPPTAIFCTSDLIAIGVMDAARFEYKLRVPED
                     LSVIGYDDIQMASWKSYNLTTIRQPLDLLIEKTINILKSLLNEENPESVVEMLKPELI
                     ERGSTL"
     gene            complement(14846..16249)
                     /locus_tag="ACOIAF_002164"
     CDS             complement(14846..16249)
                     /locus_tag="ACOIAF_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HGZ2669173.1"
                     /translation="MKMLNNFFKKINNPAPVFAQTKMERDNQTDDSLSTKPKTTFRDV
                     AGLDEVKEELFEIVDFMKSPQKYQKMGAKIPKGVLFYGPPGTGKTLLASAVAGETNSS
                     FFNVTGSEFVEKYVGVGAKRVRTLFEKARKEAPSIIFIDEIDAVGAKRHLESNNEKDQ
                     TLNQLLVEMDGFNKDSNVLIIGATNRLDLLDEALLRPGRFDRHIHIGAPNYHTRFEIL
                     KVHTDDKPIDKSVNLELLAKKTHGFNGAHLSNIANEAAIFAVRDDSECITSEHFDKAL
                     ERVIAGLESKNSALVEKEKKIVAYHEAGHALVSDIVGICPIQKISIVPRGQALGYVLQ
                     LPDEDRYIYTKDELIGKIKILLAGKASEELIFNHKSTGAKDDLKKVTEIANQMVCEYG
                     MSNLGFMTIDGNDKTFLCDKVQKEANRIVEICYKETLEMLKDNLEDLHSVSKFLFEKE
                     TMTHEELKDLIGKEAVN"
     gene            17229..17720
                     /gene="infC"
                     /locus_tag="ACOIAF_002165"
     CDS             17229..17720
                     /gene="infC"
                     /locus_tag="ACOIAF_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015526092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HGZ2669174.1"
                     /translation="MNEQIKDKEIRVLSPTGEQLGVMPTKEAQAMANSKNLDLVQISP
                     NANPPVCKIMDYGKFRYEQARKEKEAKKKQKTIVVKEVRLRPGIEQNDLNTKANNAIK
                     FLKKGDKVKVELRFRGRELGHKDIGKEVMLKFLDIIKEFGEPTKAPAFEGNNMVVIID
                     PKK"
     gene            17748..17942
                     /gene="rpmI"
                     /locus_tag="ACOIAF_002166"
     CDS             17748..17942
                     /gene="rpmI"
                     /locus_tag="ACOIAF_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HGZ2669175.1"
                     /translation="MPKMKTHRGAAKRLKKTGTGKLKRAKAFKRHKLTGKSAKTKMDL
                     RKSTLVSDGDAKRIAQLLPY"
     gene            17998..18354
                     /gene="rplT"
                     /locus_tag="ACOIAF_002167"
     CDS             17998..18354
                     /gene="rplT"
                     /locus_tag="ACOIAF_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HGZ2669176.1"
                     /translation="MARVKKAMNARKKHKKILKLAKGFRGSRSKLYRPANTFVMKALK
                     NAYIGRKLKKRDFRKLWIQRINAAARMNGISYSRLMNGLKLSGIEVNRKMLSEMAIQD
                     PEGFAKLAEVAKAKLA"
     gene            18664..19209
                     /locus_tag="ACOIAF_002168"
     CDS             18664..19209
                     /locus_tag="ACOIAF_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669177.1"
                     /translation="MNEKLMDCFVNPIKCKLLIEIFSNNRTTAKQLGEKFQDISKATL
                     YRYLNKMLKDDIIKVVEENKIRGTVEKVYAMKLDIADDVNKIIDENSGEEYAQLFTQY
                     MMGFLREFYEYAERKEKDILKDGSGFSVCPVYASIDEIVEVSKKIASILEPLRNNSHE
                     EGRRMHSIGVIITPPKEDEGK"
     gene            19196..19369
                     /locus_tag="ACOIAF_002169"
     CDS             19196..19369
                     /locus_tag="ACOIAF_002169"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669178.1"
                     /translation="MKGNKSDLHGVEPIYTGNRFMIYICIQSKVSLLGLYFIVPFHPM
                     TWSGTMKFFNFSY"
     gene            19644..21569
                     /locus_tag="ACOIAF_002170"
     CDS             19644..21569
                     /locus_tag="ACOIAF_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HGZ2669179.1"
                     /translation="MKIKFVIMPLVIFLMVLFVGCQSTKDTEVKAKDTEIKILGTGDL
                     HSILTDSMVSYVNEEREKNKNLLMVDAGDFYGTQSREMWEWSSGKKLINIKRNGRAEY
                     DDIIKSSKDEVPIVKDMAKLKYDAVVLGDNEFVLNDKQSLDKLVSDFKNNNMPLVSAN
                     IYEQSGENYVQPYVMKNIKTDEGNVKVGILGLTIKEVGETLGLEDFEKDKKARELGEQ
                     ADYEGKLYANDLVEDTKKWIKVMEKESPDIIVAVVHSGEEPKADRNPGNRIKELATTV
                     DGIDAIVAGHTHEKIDEHKYKNKSGDEVIVTQPGWHGDRVSEINFKLNKENGKWDIKE
                     KSSKLKIIDKEVSIVATADLHSHFSDKLTVDLVNERSNPIEPVVVDAGDFLDPQTDEM
                     TQWYKEWKSIRDNNSDKTISRCPMVMDMNQGMYDAVVLGNHEFVSEKDEFWSDERLLD
                     AVIEDFEQVAISVLSANIYRQSGKNYVKPYIIKDVETSEGNVKVGILGLTIKEVGKGL
                     KNVNLQEQFQYKDKLYANDLVEDAKKWVKVMKKKKPDIIVAVVHSGEEPKNPKHPGNR
                     IKELATTVDGIDAIVASHTHEKIDEHEYKNKSGEKVIVTQPGEHGKYYSKITFKVNKE
                     KGSWIINDKFSKTIEVE"
     gene            21970..23874
                     /locus_tag="ACOIAF_002171"
     CDS             21970..23874
                     /locus_tag="ACOIAF_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HGZ2669180.1"
                     /translation="MKIKFIIVASIIFLIVLLVGCQSTKDTEINVLATSDLHSIIPDN
                     LISYVEEERKYDENLLMVDAGDFFDMQSGEMNKWFTGQKLVRFKDGIPEYKKIAEPRE
                     GEVPLAKKMAKLKYDAVTFGNHEFVSNDKQSLDKLVSDFKNNNIPLVSANVYEQSGEN
                     YVKPYVMKNINTDKGTVKVGILGLTIKEVGERSRFKSFEQDKKARELGEQPQYKGKLY
                     ANDLVEDANKWVKVMEKKEKPDIIVAIVHSGEEPKNPKNPGNRIKELATTVEGIDAIV
                     AGHTHEKIEQHTYKNNSGEEVIVTQPGAHGNSISKINFKLDKKNDKWFIKDKYSELKV
                     FGKEVNIITTSGLNPKFSDEMAVNLFNERDRDVQPIFIDTGDFLNTNTKEMTKWAKEW
                     QYNENKGIYKKINEWPIVYLGYDAVVLGSHEFTEDKETFEEKKSTLDYIIENFEEMKI
                     PVLSANIYDESGENYVKPYIIHNVETREGNIKVGVLGLTTTDKDEEDLSNSSLSKDSK
                     TSKIPEETGKLYTNNLVKDASEWVKVMQKESPDVIVAVVHSDNNSTLKNPSSEVKKLA
                     MSVDGIDAIVVGHTKNKVEEHIYKNKSGDEVIVTQSGEDCYSKISLELRKENGKWNII
                     NKYSKAIKMK"
BASE COUNT         8918 a         2699 c         4384 g         7894 t
ORIGIN      
        1 atagaatcat ataaaataag ttaactacaa tcaaaaatta aaaaggggaa atgttaagtt
       61 gatagaaggc ataaaattta taaaggcaga agaaaagtat atttattcgt attggcaaac
      121 ttttgataaa atagcaaaag agaggaaata tctagctatg gatgaagcct ttccttttga
      181 agaaacagtt gaatttataa aaaatattat aaataaaaac ttaccacaac tgtttataat
      241 tgatttagaa tcagataact gtattggttg gtgtgacgtt ttacctaaaa ctgaaaaagt
      301 tggttatcta ggcatgggta ttcttaaaga gtatagggaa aaaggtatcg gaagtagtct
      361 tttaaaacaa attattgatt tatcaaaaga atatgggtat gaaaaaattg agcttgatgt
      421 atttaaaagt aatagtagag ctattcatgt ttataaatct ttagggtttg tagaggtaaa
      481 tacaatatct agtggtttta catggaatga tagacctgta aaagaagaag taattcaaat
      541 ggagcttaca cttatataaa ataaagttaa agtctttaaa attccatata cacttgtata
      601 aaataaagtt aaagccctta aaattccata taaaagaaat atttttaagg gctttttaat
      661 aatggttctt ttgaaggtag ctaaatatct aaaattttat atattaaatt taaagaaaat
      721 ataaagatgg aagatacaac catgtttact aataaaaaaa ttaaagtcat aaataataaa
      781 ctcataaaca acttattatt acttatgttt attttgcatc tattacttca tgagccattt
      841 taactaagtc attcatagat aaatttgagt cttgaactgt aaaagaatag taaactccat
      901 cttgtttcca tgtcaaaaac ttaaatttat taacttcaac tttgtctgat tcattatttg
      961 gccccacact aaatgtgtat ttgcctgatt tttcatcaat tttatcctgt tctgtcattt
     1021 tataaccttc tggtacaaac ttttgtgcga atgcttcata agaaatatta atatctttgt
     1081 aagtatctac aacttttaga ttgtcagaac tcttcccaag tacatcattt gctgtgtcaa
     1141 gagatatttc atcattgcca tttttatatt caaaactaag tgcttttgat tttcctaaaa
     1201 cattattttc tttgtcaaat gcttttttat ttacagtata tgctccttta aatttatatc
     1261 cattactaaa ttcagaaact atattagggg taaagttaaa gtcttttttt acttgttctg
     1321 tagttgggat agatgtatag gtagccttag tacttgattt tccaactaaa ctatatatat
     1381 gtcctactgc aagggctgtt gtgctcaata tcaaagtaat ggctaaagtt gcaactaagc
     1441 cttttctaaa tggtaatttg tgtgttttat tattaatcat tttataattt tcctctcttt
     1501 ctggtaaact atttagtgtt ttgcttaaac tattatgaaa actttctgga acttctggaa
     1561 acgaattatt taatatattt ttattcatat gattacctcc gtgttttcta atttagtttt
     1621 taataatctt cttcctcttg ataatctact ttttacagtt ccttgaggta tatctaatat
     1681 ttccttaact tcatctactg aataaccttc aatatagtaa aggactattg gaattttaat
     1741 ttttttaggt agactttgaa tagcagtata caaatcgcta tagtcatctt tttcatcaat
     1801 ttttttgttt ttgaagacat cttcaaatga tagagatttt aatcgtttgc gtcttaatga
     1861 attacattca tttatgacta ttctaatcag ccatgtttta aagtattgtt cttccttaag
     1921 agaatccaac ttttcatatg cttttaaaat agcattgttg atggcatctt cacagtcttg
     1981 gtcgtttcct agaatagact tagatactcg atacaaagtc tgttctgatt caagtatatt
     2041 gtttgtaaat gtagttttat ccaactaagt tccttccctt ctatgcaaat cgtagcgcta
     2101 cttttttgcc tttcacttat tagataaaat tttactatag atggttcact ttttttatga
     2161 ttatttgata aatttaagtt tgaaagtttt gcctattcta tacaaatatt ctataatata
     2221 tttttttcta gtattttccc ctcatatata aagccatgta gatatattaa actttagaag
     2281 ccatttatat atttagtttt atagtaagtg tttatattga gtaaattaga aaatagtaag
     2341 tatttaatgt tgagtagtta gaataacaat gcttttggat tatttacttc atgtacagat
     2401 ttaaaataag ttttttaaat aatgagtaaa gataacagta tcttgactaa aagtaggctt
     2461 ttaagaaaaa acaatgaaac ttaaatgaga attaagagtt agaaatgtca ttattttaga
     2521 tagagatgaa cataaataaa cttaaaaaat ataaattata aaaataataa ttttttttaa
     2581 aataataaag ttttttgatt aaaagtttta tattgaaatg tcaaaataat aaaagacctt
     2641 ataagttaat tacaggttaa aaaaataata tgtgaatttt ctgatattta ctaattatga
     2701 ataaaatatt taagtattaa tgtttttagt gagactttaa gacagataat ctcctattaa
     2761 agatactata tcttagataa aataaattaa aacaatataa aaacataaca agattacaga
     2821 aagaaagctc acaataaaat gctgaaaaat ttattttaag gaggaataaa gtatgaagtt
     2881 tcaagaaatg attaatagcc caggtctttg gattgtttct agttttttag taattatttc
     2941 agttgttcaa gcaatcgtat ttatgaaaga ggcactaaaa gaggcttcaa atcttggtat
     3001 tgaaaggaaa ggaattaaag cagcaattcg ctcagctagt gttacagcca ttggaccttc
     3061 tctatctcct gttattacat tattatcact ggtagctgtg attggcgctc ctacgacttg
     3121 gatgaggctc tgtgatgtcg gagcagctag aacagagctt ggagtaatat ctcttacatc
     3181 caatttatct ggggtagaag taggtagtgc tgcatttgga gcagaagctt tttcgtatgc
     3241 actatggggg atggctttaa ataacttagg ttggctattt gttgtattta ttttgggtca
     3301 tagaatgcgt ggaattgttg aaaagatgaa catgaagtat aatcctactt gggttaaaaa
     3361 actacttgca ggagctacag ttggattatt tgcttacctt ctttcaaatc aaataaaaac
     3421 atttgaaata ccaaaactta caccagcaat tatttcagct atagttatgc tagtattgac
     3481 aactttattt aaaaagaatc aacgattaca agaattatcc ttaggtattg ccatgttaat
     3541 tggtatgttt ggtacacaga ttatattatc gtgattaaaa cggataagag gtgataatta
     3601 tgtatgataa aaagatgata aaaataggga gaatttctat aggtcttgca attattgcaa
     3661 actttattcc cgctatctat gtaggtattc gctttggtca gatgccaatg ctttctgtta
     3721 tatttcaaat ttgggggtta gtcgctgcga cttatggggt tagttggatt gttcaaccta
     3781 tagcttatta tcctacacta ggagctccag gttcttatat aggttggctt gcaggttctg
     3841 ttggagatat acgtatgcct gcagcatcaa tggcacagaa aattgcaggt gtagaagcag
     3901 gaactcatga gggtgaagtt gttggaacaa ttggtacatg ttgttcaatc tttgtatcag
     3961 cttctatgat taccattttt accattgtag gctataaagt aattcctttg ttacctgaat
     4021 ttgttactaa ttcatttaat tatatacttc cagcattatt tggagcaatt tatgttgata
     4081 ttgcacgtaa ggatattcga gctggtggat gtacgattgc tgtagcgtta gcaattatgt
     4141 actttggtga aaaaattggt ataccaggtg gattattaac attgtttatt gtgttaggtg
     4201 ggatacttat cacaagggtc ttctttgtaa ttgataacaa taaaacaaaa gaataggatt
     4261 gcttattaag gagaaagact agaattattt gttgagaaaa aagaatagga ttgtttatta
     4321 agaaatatga atataaataa gtcttgaaag gagaaaaagc atgtttgatg ttaaaattgt
     4381 caatggaata atcgttgatg gtacaggaaa ttcaagatat aaaagtgatg tagggattgt
     4441 aggagacaaa atcatggcta ttggagattt aagtcaaaaa gaggctaaag agactataga
     4501 cgcaactgga aaatttgtat cacctggttt tatagatttc catactcatt ctgatttatc
     4561 tcttgtgtat gataaataca caagaagccg tatccataca ggtgtaacaa cagatgttat
     4621 tgcaaactgt ggtataggag ttgctccgat aagagaagag aagaagcaag agttaattga
     4681 ttatcttgga actaggataa ttggtacaat tccaacaaaa ttagagcttc attggaatac
     4741 aatgcaagaa tactttgatt acttaactga aaattctcca gcagtcaatg ttgtagctta
     4801 tgtagcacaa ggacctgtac gtatcaatga aatgggattt tcaaaggaac cagctacttt
     4861 agagcaactt aaaaatatga agttggaagt tagaaaagct atggaagctg gatgtgtagc
     4921 tctttcatca ggattagttt atttaccagg agcatacaca aagaaagaag aaatggctga
     4981 attatgtaag gaattgatac catacaatgg atactatata tctcatatta gagatgaagg
     5041 tgatgaagaa atggaagctc ttgatgaagc aatttatata gctaagactg caggagttcc
     5101 tctacatgtt tctcatttaa aagttatggg tcataagaat tttggaacta ttgatgaggt
     5161 atttaaaaag ctagatgaag ctgaggcaga tggtttggaa gtaacttttg attgttaccc
     5221 atatacagct ggaatgacat ctttgggtgc acttttacct ccttgggctt ttgaaggcgg
     5281 tgtagaaaat atggtaaaac gattagaagt acaagaaaat agagacagaa ttataaaaga
     5341 gttggaggaa gggattcctg gatggcaatg tttctaccaa ttagcaggtg ggtggaacgg
     5401 tgttgtacta gcctctgtaa tgactgaagc aaataaatat gtagagggta aaactttaat
     5461 ggaggtagcc aaaatcaatg gtgaaaatcc atttgatacg ttctttaggc ttttgattga
     5521 agaaaaaagt aaaattcaag tagttgttca tacacaaggt caagaggata cagataaggt
     5581 tgtttgccat ccaaagtcat gtataggttc tgatagtatg gacctttcta cagaaggttt
     5641 actttctctt ggaaagcctc atcctcgtgc atttggtact tttggacgaa ttttctctta
     5701 ctatgttaga gaaaaaggta tgttgacttt tgaggaagca gttaaaaaga taacttatct
     5761 ttcagcaaaa cgcttaggca tttataaaga aaggggtcta cttaaagaaa attattttgc
     5821 tgacattgtg gtatttgacc cagatacaat agaagataaa gcgacttata gtaatcctaa
     5881 acagtataca gtgggagtgg aatatgtgtt ggtgaatggt aaaattgcat tggcaggagg
     5941 aaagcaaact gatgtatgtg ctggacgtgt aattaaaaat cctcttagca tagcaaaata
     6001 gaatacatta agaactttta taaaaatagt ctttctttga atattaagga aggctatttt
     6061 tgttaaatta ataaagatta ctctacttgt gttttctaaa gaatttaact ttaagtagaa
     6121 aaaacaatat attaattgta aaatgtaatt aatattaatg aataagattt aaatacaaaa
     6181 atttaaatac aaaaatgttt caacagatag tcgtaattaa aaaacttaaa gtaacttttt
     6241 atattaggta taattgatta ttcaaaattc tatgaatcag gagtcctgtg cctttagtca
     6301 tggtaggttt agaaaatgtt ttaagagata atagtatcat taggaaagga gagacgaaat
     6361 gaaaatactt tatgtaggac caggtatggt aatcgataaa atactagcag taagtaaaaa
     6421 gaattttcct aatattgaag cagattttct tagatatgat agatatattg aagtacctga
     6481 tttattggaa aaatatagag gtagagtgga tgcagttctt tttacaggta agtcgccatt
     6541 taaatttttt gaaaaaagac aaaaagattt tgtattgtct gattatattc ctagacatga
     6601 aacaacattg tacagagtgt tgcttgaaat tacatatttg cttaaattcg atattagtag
     6661 acttagtata ggtacttatg ataaggctat gatgaagaga ttgtataaag aaattggagt
     6721 tgaatttaag gatgaacata tgttttttgc agagcaaaag tatttagaag aggactatat
     6781 aaagtttgtg cttgaatttc ataaatataa ttatcaaaat aatgatgtat gttgttgtat
     6841 tacaggtctt gaagaggcct atcatataat gaaaaaagat aatattccag tagttttagc
     6901 cttgccttct gaggacgtaa tggtgcaaag tataaaaaac attcagatgc gttatattgc
     6961 aaagaaaaac aatgagaatc agattattgt tcttgctatt aaactgaaca tgccttcaga
     7021 atattcacta atgaaggaag atgagtacgc ttatctttca caaaggatta agattcttga
     7081 taaactatac catttcaata gccgtataga tggtgttttg gtagagcaga gtagaagtga
     7141 atttatgatt tttacaacta aaaaaatact tgagttagaa acaaagaaat acagagatat
     7201 ttatttgttg gatatgattc gtgaagtttc agttataaac acttatatag gaattggtta
     7261 tggaaaaaca gcaaatgaag ctaaatttaa cgcttatgag agtattaaga tggctcaaag
     7321 tcaaaaaaat gatgcagcat atgtagtttt tgaaaatgga gaaattatgg gaccactaaa
     7381 gccaaatcct actgtcaaag agaaaaatag ttttgatgaa cgtttttatc aaattgctac
     7441 agagacaggg ctaagtgcta atactgtata taaagtattc aataaaataa taaatagtgg
     7501 aaaaactgaa tttacatcaa aggaattagc tactatatgt ggtgtaagta ttagaactat
     7561 ggacaggatt atacttagat tgtgtgattc tggttattgt gaggtagtga gtgaacagct
     7621 tatgagcaag tatggtagac ccagtagaat attacgtttt aaaccttttc tgttgtttta
     7681 aatagatata agtcaaaaat taaattttta tgataacatt ttctttacaa ctttttagat
     7741 atatgttaat atttagaaaa atgcatatgt attcataaaa taaactcaaa acagctaaaa
     7801 aatcattaaa aaggaaatcg tatgcataaa atgagaaagt taatttataa cttaaataaa
     7861 tctgggagga gataattatg ggaaaatatc aggattcaaa aaaagttgta agaaattatt
     7921 ttgaagcatt agaaaatgct actggggatg aggttgaaaa tgtcttaaag caacatatga
     7981 aatcagatta taactggaaa ggtgtgtatc catttcgtga acaagaaggc actgaaaatg
     8041 tagcagatgt attttggaag cctttaaaaa aatcattgag taatatgcaa agacgacaag
     8101 atattttcat tgcaggaaca aatgagattg acaatagtga atgggtaatg agtatggggc
     8161 attttatggg actttttgat aaggattggc ttggcattaa acatactcgc aaaatgatta
     8221 gtttaagata tgcagaattt aactgtgttc aagatggtaa aattgctaaa actggattat
     8281 ttgtagatat aattgggttt atgattcaag caggagttaa tccattgcca ccacaaacag
     8341 gttcatattt tgtatatcca gggccaattg accataatgg attacttttt gaagatgcag
     8401 atgaagttga aggagtaaaa actttagcac tagtaaataa aatggtagat gatttatctg
     8461 aattaaatga aagtggtgct atgggatgcc caccagaaat acttgaaaaa tcttgggcta
     8521 aagatatgat ttggtatggt ccaggtggaa ttggtgcttc ttatactata cctagatatc
     8581 aagagcaaca ccagctacca tttagaaata atttaaaagg aaagactttt aatgggcatg
     8641 tatgtagatt tgcagaaggt aattttgctt gtttctttgg atggcctaat ctgactaatg
     8701 taccatgtgg aggtttttta ggattaccag gtggagaggt taaagcagat atgcaagttg
     8761 ttgatgttta ttatcgtaag ggcgataaac ttcaagaaaa ctgggtgtta attgatattc
     8821 cttattggtt gaaacaacaa gggcttgata ttcttgagag aactaaaagt atttttaatg
     8881 cttagttatg catacgtatt ctaaaacaaa gggggatata aagtggcatc taccaacaaa
     8941 aataatgcaa caagtaaaga ggaagaaaaa gaagtttcta caagtgggct tgaatcatgt
     9001 acggttaaag aaaacaaagc taaaatgaat caaactaatt ttagtaatat aaatgaaaat
     9061 caaacaaatg agaataaaac gaacacatat aaaggaaata tagataaagg gaatatagat
     9121 aaaacaaaca caaataaatc cgacataaat aaagttaaca tagatacagt ggatacagat
     9181 aaattaaata caaatactct tactaaaaaa aataaaaaag ttatatttaa gggggaagat
     9241 aatatgtcat ctacaaaaat ttactatgga gattcattag aagtaacgcc tgttggagta
     9301 atggattaca atgatttttc aaaacagaca aaaagggaac aggaaatacc tggatttgat
     9361 tctaagtata gagattttgt agattatatt atgaagatta cacataatat ctgggaagaa
     9421 aaaggtatag gtgttattta tgatacatat cacaataatg ttacaatgca ttgtggctct
     9481 tcaaatttag ttggtattaa agatgttatt tccaatactc ttcaaacatt acatgcgttt
     9541 cctgatagaa gattaattgg tcaaaatgta atatggtcta actttggtgc tgatggtttt
     9601 ttatcttctc accgtgtttt gtctacagca actaacttag gtgatagtaa ttttggacca
     9661 gcaacaggta gaaaaataaa ctttagaact gtaattgatt gtgccacaac taacaataga
     9721 atccacgaag aatggttggt tagagataat ttatggattg ttactcaatt aggattaaac
     9781 cctcaggaag tagcaaaggg tatggcaaag gcaagtgaat ctaaagtgct tagtttacaa
     9841 tcaacttatg gtatctgtga atctatggat ggtcaattta tgccaactaa gtatcaagca
     9901 aaagatgatt ctgttggcga aatgatgctt gaaatgacaa gccgcattta taattataag
     9961 tatataaatg aagttaaaaa atactatcat gataatgcag tagtacattt tatatgtgat
    10021 aaagatttaa atggctatga tgagattcaa ggaatgattg taagtttact tgcatcaatt
    10081 ccaaatggaa gttatgaagt ggaaagagta acttgtaatc aaagagaaaa aaatgaaggt
    10141 tatgatgtat ctgtaagatg gagattacgt ggaataaatg aaggtatagg attttttgga
    10201 cagccttctg gaaagcatat agaagtaatg ggaatcaatc attatcatat actacaaggt
    10261 aaagtaaagg aggagtggat aacttttgat ggtatggatg tattaaaaca aatgtatatg
    10321 ggagtagaag aataaagctg tttagagtat aaatagtttt gaaatatata gggaagtatt
    10381 gctttataaa atttaagtgg ggaatgtata tgaaaaatag atgtttaaac ttttagcaaa
    10441 attatgacta aatttaaaag ggggaattat aaaaatatga aaacaacggt tgaaatagaa
    10501 aatgatatca tattaggaaa agactattca aacctaaaaa gaggaattgc atttttttca
    10561 attgctttaa tatacttttt ttattgctac aattttatgg tgggtacttt tattaaacca
    10621 acaatgattt atgctttagc agatggggga tttggttttt cattaaaaca aacagaagaa
    10681 atatttgcag ttatgtcttt tggtacaatt ccaggtacat ttatttttgg ggtgatttct
    10741 acaaaaattg ggaagaagag gactttaatt agcgttgctt tactgattgg attgacaaca
    10801 tttataccaa tgttatctcc tacaaatatt atgttatgga aaatagcacg tttgtgtaca
    10861 ggagtagtgt tgggaggcgt atttggaaca gctatgcctt tagttgcaga tatgtttcca
    10921 agtaaatatc gtggtaagtt agctgctata ttaactgctt tattttcatt agcaatgatt
    10981 tttggtggga aagtatatgg cgttttaggt gatgcaaatt ggcaaatatt gatgtatact
    11041 gcgattatac cacctatagt tggagctatc ttagcaatct tctttgtgcc agatgatttg
    11101 gagtatacaa aggaattagt taaaaaggga aaagaaactg gtgaaaagat tagctatata
    11161 agtatgtata agggaaaata cttatggatt ggattgggaa cgattctttt atcaggtgca
    11221 aatttcacag catattcagc attttcaaat aatgcaacta catacctagt gacaggaata
    11281 ggaatgtcag cagcagtggc tgggtctata tatagtttac aaggaattgg tcaattagta
    11341 ggatatttat tctggggttc aattgcagat aaatttggaa gaaaaatacc ttttataggt
    11401 atggcattag cagcagtatt tgtttttata tatacaaaat taggtggaga taatacaaca
    11461 acattctata tgatatcagt attactaggt gttgctgttg gatattctgg agcatgggga
    11521 gcatactaca cagagttgtt cccaagtaaa tatcgttcgt tgtcatctgg tatgtcattt
    11581 aatggaggaa ggattatatc tacgtttgca atacctgcta tagctggtac agcttctggt
    11641 actttaggta tgatgactat cttttacata tcaatcgcag tctttgtaac aggtgctatt
    11701 gtatggttat tcttaccaga gacgttaaat aataaaaaat aaaatactag gaatttattt
    11761 tgttacttag aaaggggtgg cttatgattt catcaattga tgtatgtatt ataataggat
    11821 atttgctatt aatgttagtt attggttatt attcaggaaa agataataaa aatcaagaag
    11881 attatttctt ggcaggtagg tcaatgccat ggattccaat tgctttatca gttgcagcta
    11941 ctatgattag tgcaaatggc tttataggtg gtcctggatg ggcatatgta gatggaatgt
    12001 atccagtaat ggttaatatt acagtacctt tagcaatatt ttttgcactg agcattacca
    12061 ccccagttat ctataaatta aaaataacct ctatatatga atatatggga cttcgtttag
    12121 gaaattattc aagaggatta actattattc aattttttat taattcattg attcaagcta
    12181 gttctatggt atatattcca gtgcttatta ttcaaatgat tactggctgg tcactttatg
    12241 ctttagtccc agtagttgta cttgtatcaa ttatatacac tctattaggt gggattaagg
    12301 cagttatatg gactgatagt attcaaatga tagtagtaat aagtgctgta tttatagtaa
    12361 ttattgtagc cttaaaagga atgaatctaa gtttctttga tacattacaa attgcacaac
    12421 agagtggaaa attaaataca tttgatttca gtagggatat ttcagtgaca aatacttttt
    12481 gggctacttt aattggtgga actgtaatgt ggataagata cttttgtttt gaccaatctc
    12541 aagtacaaag agttttaaca tctaaatctt taaaatgtgc aaaaaattct tttgtagtta
    12601 gtgcatttgt tatgaattta gtttactatt ttatgctatt ggtaggagta attttatttg
    12661 ttttttatgg gggaaggaca tttgaaactt caaatgaaat aatgattact tttattttaa
    12721 atgaacttcc agtaggagcc attggaatta ttattgcagg tgtgtttgca gcagcaatgt
    12781 ctagtatcga ctctatatta aacagtatga caactgtttt tacgaaggat atttatgagt
    12841 tttatttcaa aaaagataac aaagaatcat cattaaaagt tacaatgatt ataactgtag
    12901 ttataggagt tgttatgaca ggttttatta ttatgggatt tggaggaagc atcaaatcaa
    12961 ttcttgacct tgttggtaac tatatctcat actttgctgg accagctaca ggtgcatttg
    13021 tactagcaat gtttacatat aaagctaatg ataaaggagt atctattggt tttattgtag
    13081 ggttagtttt aggatttttt atttctaata aatataatgt gagttggttg tggaatccag
    13141 caataggtgc tacaataaca ttgatatttg gatatatttt tagtgtcatt ttaaagaaaa
    13201 atcataatgc agatgatatt aaaaaataca ctgcatttgg tatgagaaat caccttattt
    13261 ctcaaggaat ggataaagta gatggagtat ctatagtgcc attttcttta ggtaaacaag
    13321 aactaattgt attagctttt tttattatcc aatatattat attatttttg atacaatttt
    13381 aagaagaaat actagatatt taaggagaaa tactaaatga atcaaaagaa agttcattat
    13441 aataaatcgg ctacctcaaa agatgttgca agattggcag gagtatctca atcatctgtt
    13501 tcaagagcat ttggtagtgc aaatggaaag ggcgtaaaac ctgaagtaag agagagaatt
    13561 tttcgtgtag caaacgaaat agggtatgtt cctaatttgg tagcacgggg aatgattagt
    13621 ggaaaaacta atgttatagg attgatagtt ggtgatagtc taggtccatt ttacaataga
    13681 attatcaatt tatttgtaga aaaaattcaa gagataggta aacagtgtct cgtttttaaa
    13741 gttcctagac aacaaagtat agatagtata atttcaaaag taattcagtt tcaagtagaa
    13801 gctgtgatta ttacagcttc ggccatgaat aaggtgatgg ctgagacttg tgaagaaaac
    13861 aatattcctg tgattctttt taatcgtttt atacctggta taaaaataag tacagtctat
    13921 gtagacccaa ttgaaggtgg gggaatggct gctgaatatc tgcttaagaa aggtcataag
    13981 aatataggat atattcaatt tacgagagaa acaagtgaag aaatggaaaa gaaaattgga
    14041 ttttattcta agttaagaca aagtgggatt cataacctta aagaagaatc tgcaaactat
    14101 gattatgatt ctggatatga agcaggtaag agaatgttag cattatcaaa tcctccaaca
    14161 gcaatttttt gcacaagtga cttgatagct attggtgtaa tggatgctgc aaggtttgag
    14221 tacaagttga gggtgccaga ggatttatca gtgataggat atgatgatat tcaaatggct
    14281 tcatggaaga gttataattt aacgacgatt agacagccac tagatttgtt gattgaaaaa
    14341 acaattaaca tacttaagtc cttattaaat gaagaaaatc cagagtcagt tgttgaaatg
    14401 ttaaaacctg aattgattga aagaggttct acactttaag tggtagatat ttaattaagt
    14461 ggtagatatt taattaagta gtagatacct aattaagttg tagatatctg ataagaaatt
    14521 tttaagtttt caatcgttct aaattgtgag tataaaaatc tactaaattt agttatgtaa
    14581 aatataaatt ataagtgtag aaactaggct actagctttt ataccacttc aaacattact
    14641 ggaggtagtt aagtttacag cagtttagtt ttatgtagat ataatattct gtaaatagac
    14701 aaaattaaaa tatgtaagtg tctcaaaatg aaatttttag tttatgaggc acttttttgt
    14761 ataaaatcaa atgtattttt ataactttaa caatgaaaag aggcttatct ctattttgag
    14821 acagcctcaa atttttaaaa ctatattagt ttacagcttc ttttcctatt aaatctttta
    14881 actcttcatg agtcatagtt tctttttcaa ataagaattt agaaacagaa tgtaaatctt
    14941 caagattatc ctttaacatc tctaaagtct ctttataaca aatttcaact atacgatttg
    15001 cttccttttg aaccttatca cataagaaag ttttatcatt tccatcaata gtcataaatc
    15061 ctaagttact cataccatat tcacaaacca tttggttagc tatttcagtg actttcttta
    15121 agtcgtcctt tgcaccagta gatttatgat taaatattaa ttcttctgaa gcctttccag
    15181 caagtaatat ttttattttt ccaatcaact catccttagt ataaatatat ctatcttcat
    15241 caggtaattg aagaacataa cctaatgctt gtcctctagg aactatagat attttttgta
    15301 taggacaaat tcctactata tcacttacaa gagcatgtcc agcttcatga tatgctacta
    15361 tttttttctc tttttcaacc aatgcagagt ttttagattc tagccctgca ataactcttt
    15421 caagtgcctt gtcaaaatgt tctgatgtaa tacactcact gtcatctcta actgcaaata
    15481 tagctgcttc atttgctata tttgaaagat gagccccatt gaaaccatga gtttttttag
    15541 ctaaaagttc tagatttaca gatttgtcta taggtttgtc atctgtatgt acctttagaa
    15601 tttcaaatct agtgtgatag tttggtgcac ctatatgaat gtgtctgtca aatcttcctg
    15661 gtctaagtaa agcttcatct aataagtcaa gtctattggt agcacctatt attaatacat
    15721 tggaatcttt attaaatcca tccatttcaa ctaaaagttg gtttagtgtt tggtcttttt
    15781 cgttgttact ttcaagatgt ctctttgctc ctacagcatc tatttcgtct ataaatatta
    15841 tacttggagc ttcttttcta gctttttcaa atagagttct aactcttttt gctcccacac
    15901 ctacatattt ttcaacgaac tctgaaccgg taacattgaa aaatgatgag tttgtttcac
    15961 cagctacagc agatgcaagt agggttttac ctgttcctgg aggaccgtaa aataatactc
    16021 ctttaggtat ttttgcaccc attttttgat atttttgtgg tgacttcata aaatcgacta
    16081 tttcaaatag ttcttctttt acttcgtcta atccagcaac gtctctaaat gtagtctttg
    16141 gttttgtaga aagtgaatca tctgtttgat tatctctttc catttttgtc tgtgcaaaga
    16201 caggagctgg attgttgatt ttcttaaaaa agttgtttag cattttcata ttcacacctc
    16261 aggtaattaa ttattgttaa tcaattttat attttccata tttactattt tttattattt
    16321 atttttcatg gaaaaaatat acatgttatc taaataaaaa taaatttgta agatgtatga
    16381 aaaaatgaat ttatgtatat agagatttct acttatgtat tgaataaagt attacaaatt
    16441 ataaattatg tattgattat atattgagta aagtattata aagcatgtat tgattaagta
    16501 tttattaagt attgaataaa atattacaaa tttagttgaa atgaaaatga taaaaaatgt
    16561 tgtactatat tttacaaatc atataaatag agaaattgtt gtagcacaaa gtagtaggta
    16621 gtatattaga atataatata ggatttgttg tgtagtatag tagcatatta gaatataata
    16681 taggatttgt tatgtagtat agtagcatat tagaatataa tgtaggattt gttatgtagt
    16741 atagtagcat attagaatat aatgtaggat tgttatgtag tatagtagta tattagaata
    16801 caatatagaa tttgttgtgt agtatagtgt tatattagaa tacaatatag gatttgttat
    16861 gtagaatata tagagtcatg tagaatataa atatataaaa ggattttggg tataaattta
    16921 aaaattaata gcaaaatgag tagtatagac atataatgag tagtaggaag taattaaatt
    16981 tttcctaaac aaaaaaaaat agttgcaaat aatttgatta agtgatatag taaataaagt
    17041 aagaataaaa caaagaagaa gtccgcttct caccttacgc cggaggttag taaggtaaat
    17101 ataagttaaa gcttaatttt aatttaagtt tgtttaaatt tggtaactta tattgcggat
    17161 gattacgtca tccgcttttt attttataaa aattttggag gtgtcctaca attagcaaag
    17221 aattagcaat caatgaacaa ataaaagata aggaaataag agtcctttca ccaacaggtg
    17281 aacaacttgg agtaatgcct actaaagaag ctcaagcaat ggctaatagc aaaaacttag
    17341 atttggttca aatctcgccg aatgcaaatc caccagtatg taagataatg gattatggaa
    17401 agtttagata tgagcaagcg agaaaagaga aagaagctaa gaaaaaacaa aaaactatag
    17461 ttgtaaaaga agtaagactt agaccaggta ttgaacaaaa tgatttaaat acgaaagcta
    17521 ataatgctat aaagttcctt aaaaaagggg ataaagtcaa agttgaactt agatttagag
    17581 gaagagaatt aggacataaa gacataggta aagaagttat gcttaagttt ttagatataa
    17641 taaaagagtt tggagaacca acaaaagccc ctgcatttga ggggaataat atggttgtaa
    17701 ttatagatcc aaaaaaatag atgattaact gagaggagga atttgtcatg cctaaaatga
    17761 aaactcatag aggtgcagca aagagattaa agaaaacagg aactggaaag ttaaagagag
    17821 caaaagcttt taaaagacat aaattaactg gaaaatctgc aaaaactaaa atggacttaa
    17881 gaaaatcaac tttagtaagt gatggagatg ctaagagaat agcacaatta ttaccatact
    17941 aagatacaga ttataaataa taactataga agagggatat aaaaggaggt cataacgatg
    18001 gcaagagtta aaaaagctat gaacgctcgt aaaaagcata agaaaatatt aaaacttgca
    18061 aaaggattca gaggatctag aagcaaatta tatagaccag caaatacatt cgtaatgaaa
    18121 gcattaaaaa atgcttatat aggaagaaag ttaaagaaga gagacttcag aaaactttgg
    18181 atacaaagaa taaatgcagc tgctagaatg aatggaatat cttattcaag attaatgaat
    18241 ggattaaaat tatctggtat tgaagttaac agaaaaatgt tatctgaaat ggctatacaa
    18301 gacccagaag gatttgcaaa attagcagaa gttgctaaag caaaattagc ttagtattga
    18361 atcatgagat gtaaagtctt tgagttatat aaataattca aggacttttt tattttttga
    18421 aaaatactta ttagtgtcat gtacaaccaa ttttataatg ataaataaaa taagcaatac
    18481 aatagaattt tatgagattt attataagaa ttgttacaat tatttatgat agctataaaa
    18541 agtcatagta aaaatgatat tgatgtatgt tgatatatgt ataaagtttc caatcaatct
    18601 tgaattaata ttatcaacat gataatatta tcttattaat aatataaata agggggagat
    18661 aatatgaatg aaaaactaat ggattgtttt gtaaacccaa ttaaatgcaa gttattgatt
    18721 gaaatttttt caaataatcg aactacagct aaacaattag gagaaaaatt tcaagatata
    18781 tcaaaggcaa cactttatcg ttaccttaat aaaatgttga aagatgatat tataaaagtg
    18841 gttgaagaaa ataaaataag gggaactgtt gaaaaagtat atgctatgaa gttagatata
    18901 gctgatgatg tgaacaaaat tatagatgag aactctggag aagaatatgc tcaactgttt
    18961 actcaatata tgatgggatt tttaagagaa ttttatgaat acgctgaaag aaaggaaaag
    19021 gatatcctaa aagatggttc aggattttct gtctgtccag tatatgctag tattgatgag
    19081 atagttgaag tgagtaaaaa aattgctagt atactagaac ctctaagaaa taactcacat
    19141 gaagaaggta gaagaatgca ttctatagga gtgataatta ctccacctaa agaggatgaa
    19201 gggaaataaa tctgatttac atggggtaga gccaatttat acagggaatc gttttatgat
    19261 ttacatatgt atccagagca aagtatctct gcttggattg tacttcattg taccatttca
    19321 tccaatgact tggagtggta caatgaagtt ttttaatttt tcatattaat gctaaagaaa
    19381 tgaactttag accacatatg caattaggtt gcattataca attagagcac atatacaaaa
    19441 taaaaatcag tatttttata gcagataaat tgactgttat tcttcttaaa aatcatctat
    19501 tagttattgt tagcatattg aaagaaatta tagtaatcta ttgaataaat gaatattgcc
    19561 cataatcata taaactgcta tagtttaaaa taaagcagga cgttaaattt tatagtaatc
    19621 taaaaattta agagggggat tttatgaaaa taaaatttgt tattatgcca ttggtgatat
    19681 ttttaatggt attatttgtg gggtgtcaaa gtacaaaaga tacagaagtt aaagcaaaag
    19741 atacagaaat taaaatatta ggtacaggtg atttgcattc aattttgaca gactctatgg
    19801 tttcatatgt aaatgaggaa agggaaaaga ataaaaatct gcttatggta gatgcgggag
    19861 atttctatgg tacacaaagt agagaaatgt gggaatggtc ttctggaaaa aagttaatta
    19921 atattaaaag aaatggtaga gcagagtatg atgatataat taagagtagt aaagatgaag
    19981 tacctatagt taaagatatg gcaaagttga aatatgatgc tgtagttttg ggagataatg
    20041 aatttgtttt aaatgataag caaagtcttg ataaactggt ttctgatttt aaaaataata
    20101 atatgccatt ggtatcagca aatatatatg aacaatctgg agaaaattat gtacaaccat
    20161 atgtaatgaa aaatattaag acagatgagg gcaatgtaaa agttggaata ctaggtctta
    20221 ctataaaaga agtaggggaa acccttggtc ttgaggattt tgaaaaggat aaaaaagcaa
    20281 gagagcttgg agaacaagct gattacgaag gaaaattata tgcaaatgat ttagtagaag
    20341 atacaaagaa atggataaaa gttatggaaa aagaaagtcc agacataata gttgcagttg
    20401 tacactcagg ggaagaacct aaagcagata gaaatccagg aaatagaata aaagagttag
    20461 caactacagt ggatggaata gatgcaattg tagctggtca tactcatgaa aaaatagatg
    20521 aacacaaata taaaaataaa tctggagatg aagtaattgt tacacaacca ggttggcatg
    20581 gagatagagt atcagagatt aatttcaaat taaataaaga gaatggaaag tgggacataa
    20641 aggaaaagtc tagtaaatta aaaataattg ataaagaagt aagtatagtt gctactgcag
    20701 atttacattc acatttctct gataaattaa cagttgacct tgttaatgaa cgtagtaacc
    20761 caattgaacc tgttgtagta gatgcaggcg acttcttaga ccctcaaaca gatgaaatga
    20821 ctcaatggta taaagagtgg aaaagtataa gagataataa tagtgataaa acgattagta
    20881 gatgcccaat ggtcatggat atgaatcagg gaatgtatga tgcagtagta cttggaaatc
    20941 atgaatttgt atctgaaaaa gatgaatttt ggtcagatga acgcctttta gatgctgtta
    21001 tagaggattt tgaacaggtg gcaatttctg tgttatcggc aaatatatat aggcaatctg
    21061 gtaaaaatta tgttaaacca tatattataa aagatgtgga gacgagtgaa ggtaatgtaa
    21121 aagtaggaat attgggactt actataaaag aagtaggtaa aggtcttaaa aatgtaaact
    21181 tacaagagca atttcaatat aaggataaat tgtatgcaaa tgatttagtg gaagatgcaa
    21241 agaaatgggt aaaagttatg aaaaagaaaa aaccagatat aatagttgca gttgtacatt
    21301 caggagaaga accaaaaaat ccaaaacatc caggaaatag aataaaagaa ctagcaacta
    21361 cagtagatgg aatagatgca attgttgcta gtcatactca tgaaaaaata gatgagcatg
    21421 aatataaaaa taagtctgga gaaaaagtta tagtgacaca accaggtgag catggtaaat
    21481 attattctaa aattaccttt aaagtaaata aagaaaaggg ttcgtggatt attaatgata
    21541 aattcagtaa aacaattgag gttgaataac tttataaaat ttatagataa agtaaattat
    21601 ctgatattga catgatatat aagataaagt ttctaatatt agtataatgc ataatgtgaa
    21661 atattttaat aataagagaa taaacaagac aaataaaaat atttgaacat gtaagatata
    21721 gtatgaacta ttatactaat gcttacttta taatttgaac atgttagaat aagtcttatt
    21781 tgaaaatttt agtgtattag agtaagtatt attttaccag taaaaaatat tgagaactat
    21841 atgtagattt gcttaaaaag gaatttacat atagttcttt tcgtataata aatagtacaa
    21901 tcttatatgg taaagataaa gtgtgatatt aaattttatt atcatccaaa atctaaggag
    21961 gagtctctta tgaaaataaa atttattata gtagcatcaa taatattttt aatagtatta
    22021 cttgtggggt gtcaaagcac taaagataca gaaatcaatg tactcgccac aagtgattta
    22081 cattcaatta ttccagataa tttaatttcc tatgtagaag aggagagaaa atatgatgag
    22141 aatctactta tggtagatgc aggagatttt tttgacatgc aaagtggtga gatgaataag
    22201 tggtttactg gtcaaaaatt agtaagattt aaagatggga taccagagta caaaaaaata
    22261 gctgaaccac gtgaaggaga agttccattg gctaaaaaaa tggcaaagct gaagtatgat
    22321 gcagttacat ttgggaatca tgaatttgta tctaatgaca agcagagcct tgataaattg
    22381 gtttctgatt ttaaaaataa caatatacca ttagtatcag caaacgtata tgaacaatct
    22441 ggagaaaact acgtaaagcc atatgtaatg aaaaatataa atactgacaa gggaactgta
    22501 aaggttggta tacttggtct gactataaaa gaagtagggg aacgttcaag gtttaaaagt
    22561 tttgaacaag ataaaaaggc tagagaactt ggggaacaac ctcaatacaa agggaaatta
    22621 tatgcaaatg atttagtaga agatgcaaat aagtgggtaa aggttatgga aaaaaaagaa
    22681 aagccagata taatagttgc cattgtacac tcaggtgaag agccaaaaaa tcctaaaaat
    22741 ccaggaaata gaataaaaga actagcaaca actgtagagg gaatagatgc tattgtagca
    22801 ggtcatacac atgaaaaaat agaacagcat acatataaaa ataattctgg tgaagaagta
    22861 atagtaacac agcctggagc tcatggtaat agtatatcaa agattaattt taagttggat
    22921 aagaagaatg ataagtggtt tataaaagac aaatatagtg aattaaaggt ttttggaaag
    22981 gaagtaaata taattacaac ttctggtctg aatccaaaat tttcagatga aatggcagtt
    23041 aatttgttta atgaaagaga tagagatgtt caacctatct ttatagatac tggagatttt
    23101 ttaaatacta atacaaaaga aatgactaag tgggctaaag agtggcaata caatgaaaat
    23161 aaaggcatct ataaaaaaat aaatgaatgg ccaattgtat atcttggtta tgatgctgta
    23221 gtacttggta gccatgaatt tacagaggat aaagagactt ttgaggaaaa aaaatctaca
    23281 ttggattata ttatagaaaa ctttgaagaa atgaagatac cagtattatc agctaatata
    23341 tatgatgaat ctggagaaaa ttatgtaaaa ccttatataa ttcataatgt agaaactagg
    23401 gaaggaaata taaaggttgg agtattaggt cttactacta ctgataagga tgaagaagac
    23461 ttaagtaact caagcttaag caaagactca aagacttcga aaattcctga agaaacagga
    23521 aagttatata ctaataatct agtaaaagat gcaagcgaat gggtaaaagt tatgcaaaag
    23581 gaaagtcctg atgtgattgt tgcagttgta cattcagata ataatagtac tctaaaaaat
    23641 ccaagtagtg aagtcaaaaa acttgcaatg tcagtagatg gaatagatgc tattgttgta
    23701 ggtcatacga aaaataaggt agaagaacat atttacaaaa ataagtctgg ggatgaggtt
    23761 atagtaacac aatctggaga agattgttat tcaaaaataa gtcttgaact aagaaaagaa
    23821 aatggcaagt ggaatatcat aaacaaatat agtaaagcaa taaaaatgaa gtgaatttat
    23881 tgatatataa accat
//
LOCUS       DBGJFF010000056        23858 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.057, whole genome shotgun sequence.
ACCESSION   DBGJFF010000056 DBGJFF010000000
VERSION     DBGJFF010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23858
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.057"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            86..1411
                     /locus_tag="ACOIAF_002172"
     CDS             86..1411
                     /locus_tag="ACOIAF_002172"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HGZ2669181.1"
                     /translation="MRMYDIIKKKRDNLELSKKEINYFIENYSKGDIPDYQASALLMS
                     IYLNKMNKQETVHLTEAMMNSGDMINLSEIDGIKVDKHSTGGVGDKTTIALIPLVASC
                     GAPVAKMSGRGLGHTGGTIDKLESIPGFSTEMEISKFIDSVNKSKIAVCGQSAKVAIA
                     DKKIYALRDVTATVDNISLISSSVMSKKLASGADAIVLDVKTGDGAFMKTLEESFELA
                     KSMVDIGTGMNRDTIGIVTDMDEPLGFAVGNSLEVIEAIETLKGNGPKDLVELCEVLG
                     AYMLVLSKVAHDFEDGVEKIRESIKSGTAIQKLKEFIENQGGNKDVVDDYSLFKKSKY
                     KYPVKSTKSGYISKIKAEDVGVSAMILGAGRETKDDILDLSAGIILEKKVGDYVNEGE
                     VLAYMYYNDEQKFSLAKGRFIDSYSIVDSKIEKNKLIYGIVTKEEIKKF"
     gene            1624..2703
                     /gene="mltG"
                     /locus_tag="ACOIAF_002173"
     CDS             1624..2703
                     /gene="mltG"
                     /locus_tag="ACOIAF_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HGZ2669182.1"
                     /translation="MNFKENRLKIAVLIIVILIILAGIFVFIQIGPYDKNNKKDVIID
                     VPSGASVGKISDILYENKLIKNELLFKLLVKVSNKAPSIKSGTYLLNQSYSNNDIISL
                     LVSGKIYQDGIKVTIPEGATSKEIIAMLVSKNLGDKATFENLIKKPQEFYDKFPYLKE
                     DGITSLEGFLYPETYYFNSKKQSEEDILSEMLKVFDSKYTDKFKKKQKELNMTLQEVM
                     EMASIIEKEAVLDKDRPIIASVFYNRLKVGMPLQSDATIQYIFEERKKIVTYDDLKID
                     SPYNSYKNKGLPPTPISNPGIKSIEAALYPEKTDYLYFVAKIDGGNNYSTNYQDHLKY
                     VKEYKEARDKQSKDTKTTNKENTKK"
     gene            2875..3549
                     /locus_tag="ACOIAF_002174"
     CDS             2875..3549
                     /locus_tag="ACOIAF_002174"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HGZ2669183.1"
                     /translation="MSNIVNDLVEDYIRVTLKEKEGFLKDLEIYAEENSVPIIHKEVS
                     DLLKVLLKVQKPKRILEVGCAIGYSSIFFATIIGKDADIITVERNEKMIEKAKENIKL
                     AGFDNNITILEGDAEEKLSQVQGEFDLIFIDAAKGQYKLFFDLVIDKLKDGGLLVSDN
                     ILYKGMVAHDDFVVRRKKTIVKRMRNYLDYICNCDYLSTSLVPIGDGVALSYKESKRG
                     DVDGIK"
     gene            3536..4783
                     /locus_tag="ACOIAF_002175"
     CDS             3536..4783
                     /locus_tag="ACOIAF_002175"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HGZ2669184.1"
                     /translation="MELNKVELLAPAGDLERLKIAITYGADAVYIGGEIFGMRSAAKN
                     FSKEDMAEGVAFAHERGKKVFVTVNIIPHNEDFLQLEDYLLELEEIGIDAVIIADPGV
                     LSVIKKVIPNMEIHLSTQANTTNYLSANFWYEHGIKRVVVARELSFDEISEIRAKTPL
                     DMDIEAFMHGAMCISYSGRCLISNYMTGRDANKGSCAQSCRWQYHLVEEKRPGEYFPI
                     YEDERGTFFFNSKDLCMIEYIPELIKSGITSLKIEGRMKTAYYVATVVRAYRMAIDEF
                     YRDPENWKFNPMWMEELKKGSHRHFTSGFYLNKPTTEDQNYQSASYVRNYDFIGIVRE
                     TEDEDGLIVVEQRNKMCVGDEIEVMGPYKETMFTKIEEMYNEEGEAIESAPHPRQIVK
                     LKLSVKVGKDYMLRKVIEEKVEE"
     gene            4855..6519
                     /locus_tag="ACOIAF_002176"
     CDS             4855..6519
                     /locus_tag="ACOIAF_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HGZ2669185.1"
                     /translation="MSKKKTPFLKKVGKRSWCIFTIILIIYSVLIYRLVDIQVLKGDK
                     YKQSVESQSVEKVELNSGRGIIYDRNNKKLTDTSKSQVLIVEKEKLNNNYKILELIKK
                     ATKMNDLDIYKAVQEQLTRPIIQIQTKNIDKSMKKELEKNGIMVEEKTMRYAKDGLLS
                     HTIGYIKEDDKSGQSGIEKSMDSVLRNSNEKYISAFKAGDAGNEKSLNILKGSVKTVD
                     NKDKDRHLKTTIDYNIQKKLEQILNKEENPTAAIISEASTGEILAMCSRPNFDQNDIS
                     KSLKGKNGEFENRVIKATYPPGSVFKMVVLFSALENGVIDENYTYNCTGKTKVGNTNE
                     ILRCNKRDGHGFQNLRQAFSNSCNPAFLDIAMKLGKEKILKSAEKLHLFEKVDIGLDE
                     EKIREAPKNISIRNLAIGQENIEFTPLQINQMTQIIANNGTFKPLYLYKSLVDNNMNT
                     IKTYKSSKKEELISPYVCTQVKEYMKSVSRIGTAKDLKDIEGGCGVKTGTAQSSLNKK
                     AIDHGWITGFYPEERPKYVITVLVEGTQKGNKSATPIFKEICESIK"
     gene            complement(6616..7257)
                     /gene="sigK"
                     /locus_tag="ACOIAF_002177"
     CDS             complement(6616..7257)
                     /gene="sigK"
                     /locus_tag="ACOIAF_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigK"
                     /protein_id="HGZ2669186.1"
                     /translation="MAALKSFEKPLTPEEEIEYLTKFKIENDKSAKDTLIERNMRLVA
                     YIAKKYNNSTEDQDDLISIGTIGLIKAIETYNIDKGTRLATYASRCIENEILMNIRSN
                     KKNKTQVSLQDPIGTDKEGNEISLLDILGTEANYVLDEVELKVQVGKLYEQLNKILTP
                     REREIVQLRYGLTPYGYKTQREIAQKLDISRSYVSRIEKKALKKLEKELVQES"
     gene            7824..8186
                     /locus_tag="ACOIAF_002178"
     CDS             7824..8186
                     /locus_tag="ACOIAF_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669187.1"
                     /translation="MKIRKSGFISIECIISIAILYVAVYLVSTSLYNCYSFISRNISD
                     REMLSTAKKYIEDEKYRIQNSKYELIKDKIEKNYINGYEINSRIEQILDYYQCYEINI
                     EIKNEFKKLRFNSYVTRK"
     gene            8170..8877
                     /locus_tag="ACOIAF_002179"
     CDS             8170..8877
                     /locus_tag="ACOIAF_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669188.1"
                     /translation="MLLENKGSILIITLIIFSIISTVCIMCIGLIYTHNNMFELGYKD
                     IQLKEISLSGIEISVSNVLSCVEEAIDVSNNEDEFREYFLGNNMIECIKKIKDTSYSS
                     LEEISLKVENNTIYDRGDFFEFDIESNVREKEFLKAIKVRVKIKNPWIGIDIEDYMDK
                     SNNNKIDDKTFNNYKASENNEILNQFKNESENIRNQEVVEFTEEDEEELKQNIIENIK
                     KNFDEKNLVQVYKYRGV"
     gene            8897..9316
                     /locus_tag="ACOIAF_002180"
     CDS             8897..9316
                     /locus_tag="ACOIAF_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669189.1"
                     /translation="MTVIELIVSISIILLIVTLSFPKDNLENHKINLFARQLCSDIRY
                     VRRINMFSNYNTYIYYINQGKHEGYVLRKDGKNIKSIMLPKNAKIAKSIEIIKFKSDG
                     SPQKASTIEIYNNRLRKTITITPVSGRVLLKEGKYET"
     gene            9306..9830
                     /locus_tag="ACOIAF_002181"
     CDS             9306..9830
                     /locus_tag="ACOIAF_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669190.1"
                     /translation="MKHKYGYLLLESVVSLSSMVIIILVLYSIFLSTINLKLKVEDKI
                     ELQQQSLEIIKSMEGIISNSMGIMNISNYEETFKKTTSIKCRYVDENNNEEGISNKEI
                     ILNERRNKLFVNSLNGESSQAGGYEIGDYVDEMYVSITNNGQYVNIKLKLSKRSQKYE
                     TDFKIKVWNFSESI"
     gene            10142..10699
                     /gene="efp"
                     /locus_tag="ACOIAF_002182"
     CDS             10142..10699
                     /gene="efp"
                     /locus_tag="ACOIAF_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HGZ2669191.1"
                     /translation="MVSAGDFRKGVTFEKDGQPCLVVDFQHVKPGKGAAFVRTKYKNL
                     KTGAIREESFNPSEKFPKAVIDTRQMQYLYNDGELYYFMDQENFEQIPLNYEQVEDAI
                     KFLKENEVATIRFYQGQPFQVEAPNFAELEVTDTEPGIKGDTASNVTKAATVETGAVV
                     QVPLFINTGDKVKIDTRTGEYLSRV"
     gene            10791..11246
                     /locus_tag="ACOIAF_002183"
     CDS             10791..11246
                     /locus_tag="ACOIAF_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1247 N-terminal domain-containing protein"
                     /protein_id="HGZ2669192.1"
                     /translation="MKHLYEEVAYLKGLAEGLEISAESKEGKMIHKIVDALEVFADAI
                     VTLDEEQEELQDFVESIDEDLADLEEDIADMEEDLYEEDDDEDDEDFSYIEMECPNCG
                     ELVEIDEDLLYDDEVDVVCPDCKAVILSSEDDCDDDCTCGGCSSCDDRE"
     gene            11409..12119
                     /gene="rnc"
                     /locus_tag="ACOIAF_002184"
     CDS             11409..12119
                     /gene="rnc"
                     /locus_tag="ACOIAF_002184"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HGZ2669193.1"
                     /translation="MKISKKLLDNIQRFENVINYKFKNKEYILEALTHSSYSNENKKY
                     NFNERLEFLGDSVLGIVISDYLFNEEANLPEGELTKLRANIVCEDSLSEVANDINLGI
                     HMLLGRGEEATGGRHRTSILADAFEAVIAAIYLDGGFESARQFILHHMENIIYDSRKG
                     NIFRDYKTHLQEVLQGNGENNIWYRLIEEKGPDHNKRFVMEVGINDDVLGIGEGKSKK
                     EAEQLAAKIALKKKLWEK"
     gene            12173..13243
                     /locus_tag="ACOIAF_002185"
     CDS             12173..13243
                     /locus_tag="ACOIAF_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006448 - regulation of translational
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongator complex protein 3"
                     /protein_id="HGZ2669194.1"
                     /translation="MKKRIIPIFVPHKGCPHDCIFCNQKKITGVSTDVTSEDARNIIE
                     ECLETIDKDADVEIAFFGGSFTAIDIDIQKSLLSVAKEYVEKGLVKDIRMSTRPDCID
                     EDILSMLKEYKTSIIELGVQSLDEKVLLDSIRGHQSEIVYKSSKMIKNSGIKLGLQMM
                     IGLPADTEEKCIFTAKKFVELKPDCVRVYPTLVVKDTGLEKLMEQNKYNPFTLEESVN
                     IVKKVLVLFYVNNINVIRVGLQATDDIQIGKAVLAGPYHPAFRELVEADMIKDYLEFV
                     ILQNKNIKQILVKSNKKNISKIIGNKKTNVKYMEEKFGVLLKTQESDLDINQLEIVLD
                     GKSLISANMRDIHRKLYDIYDL"
     gene            13271..16825
                     /gene="smc"
                     /locus_tag="ACOIAF_002186"
     CDS             13271..16825
                     /gene="smc"
                     /locus_tag="ACOIAF_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HGZ2669195.1"
                     /translation="MYLKRLELKGFKSFPVKTDIIFKEGITAIVGPNGSGKSNISDAV
                     RWVLGEQSIKSLRGDKLEDVIFAGTDTKKPMNYCEVALTIDNSENQLELDFTEVTIRR
                     RAYRNGESEFFLNNKSCRLKDIKEVFLDTGIGKDGYSIIEQGKVDEILSNNPLSRRKV
                     FDEACGISKYRYKKQEAERNLSNTKENLERIDDVYIEIENQLKPLFNQQTKAKKYLEI
                     SEKLKTLEVNSFIREIEGIEKELSEVNEHRKVIEKELNEKEEQKNVVEKKQEDINKEV
                     EVLQDVIEKSVDYINSIKGVISKKESQINLIKERIRNFTNEISRKNLEINDIKEKLNE
                     NKQYIKELESNKLSGSEELSTLQENIKVLEASKDKQKIKLESLNNEIELLKESIIDIL
                     NKKQEFSNKLSTLNANKENMNIRDENINSEITELNKNIEIKSSELDTINKEFNMQNEN
                     LKNVNNRHKELSINLQDSISEHNKLEDEIQKSKYNLNGYNSKLNVYIDMENHYEGFNR
                     GVKEVLKNKNLKGVHGALGQIINVPEKYEKSIEAALGAYMQNIITDNEFSAKSAINYL
                     KQNNLGRVTFLPLNIIKSNKISLANLKANTKFIGIASDLIRFDEKYRNIIENILGRTI
                     LINNIDEGIKFAKETGHRFKIVTLDGEILNPGGSLTGGSLKTNGNILSRKRYINEYTE
                     KISNIKNEISHLELKRESLDKDVKNIKNEIDSHESKIKDLEKSIIIKSTSIKNVESEI
                     ESLKGSITKLENEKNDLNSNLNYTLEKSDDVRKDMEELDDLYNKNKEKIDALNEEIKR
                     YNDLYDKEKSEFDELNLSLVKKTEVYNSIVRDIKRISGENCELEEKNKQLEESLNYEE
                     HEIIKLQDSILTEEKEKENLTKQLGDSNRNLETRKIAKDDLKNSFDEINKELKTIDRQ
                     HIELKESLFKVGGRLERLKTSQDTYINKLFEQYDMTLVQALEIKDEDLDIDRKFLESL
                     KREIRSLGNINIDSIKEYEEIKERYDFYSEQKQDLEESMEEIEKLIHTLEENMKSEFE
                     IKFEEISKNFKYVYKRLFGGGCGELTILDKENLLESDILITAQPPGKKMKNLNLLSGG
                     EKALTAISILFAILITKPTPFCILDEIEAPLDDANIFRFGEFLKDLSKETQFISVTHR
                     RGTMEAADYIYGVTMQEKAISKVISLKLKEAQEITDII"
     gene            16852..18132
                     /gene="ftsY"
                     /locus_tag="ACOIAF_002187"
     CDS             16852..18132
                     /gene="ftsY"
                     /locus_tag="ACOIAF_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HGZ2669196.1"
                     /translation="MLKKLFGFGKDKEKEIEKKDAEEEIEVEDSVDNLENLEETIFSG
                     LEEEVIDKVEDIEEKNEESVNNEGTEEIENFEKIEVEMDSNGKEVENISNNDSQEEII
                     EELENYDEIESKEIEDKEDKKVNLFERLKQGLTKAKQGITDRIDEVLKSYTKIDEELL
                     EDLEEILITADVGVNTTMDIIERLRDKIKQKGITEPIKVREELKSIVEDILTNENSTL
                     DIEPAPCIILMVGVNGVGKTTTIGKLANRYKKDGKKVLLAAADTFRAAATEQLEIWAN
                     RTNVDIIKHQEGADPGAVVFDAIKAAKARKTDVLICDTAGRLHNKANLMNELGKVFKI
                     VDREFPEAKREVLLVVDATTGQNAVVQAKTFKEVADITGIVLTKLDGTAKGGVVLAVK
                     SEVDVPVKLIGVGESVEDLQDFNAKSFSDALFGN"
     gene            18235..18603
                     /gene="ylxM"
                     /locus_tag="ACOIAF_002188"
     CDS             18235..18603
                     /gene="ylxM"
                     /locus_tag="ACOIAF_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048500 - signal recognition particle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxM family DNA-binding protein"
                     /protein_id="HGZ2669197.1"
                     /translation="MNIEKMVEIGLLFEQYKELLTDKQKEIVALYYEEDYSLGEISEN
                     LNVSRQGVYDTLKRSEKILRDYEEKLHLVSKIQEQEKNIKIIKDKIIDIKEDLLHNRD
                     CANLIPKLENIEDVCREMIK"
     gene            18600..19952
                     /gene="ffh"
                     /locus_tag="ACOIAF_002189"
     CDS             18600..19952
                     /gene="ffh"
                     /locus_tag="ACOIAF_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HGZ2669198.1"
                     /translation="MIFEGLSDKLQGAFSKLKSKGKLTEVDVKNAMREVKLALLEADV
                     NFKVVKDFIKKVQERCVGQEVMQSLTPAQHVIKIVNEELTSLMGDVQSKVMISSKPPT
                     ILMMVGLQGAGKTTTAGKLGGYFKKQGKKPLLVACDIYRPAAIKQLQVVGEKLDIPVF
                     NMGDKENPVNIAKAGLSHAIKNANDLVIIDTAGRLHIDEVLMDELKSIKSEVKPHEIL
                     LVVDSMTGQDAVNVAESFNEALGVDGVVLTKLDGDTRGGAALSIRAVTQKPIKFMGMG
                     EKLDDIEPFHPDRMASRILGMGDVLSLIEKAQENIDLEKAKELEQKIKKQELDFEDFL
                     QQMEQIQNMGPLDKILGMIPGMGNVKDQLGDVDLNGKEMKRTKAIIQSMTTYERRNPG
                     VLNASRKKRIAKGSGTSVQDVNRLIKQLNEMKKMMKMFAGSQKSMKKRGGLGGLPFFK
                     "
     gene            19990..20262
                     /gene="rpsP"
                     /locus_tag="ACOIAF_002190"
     CDS             19990..20262
                     /gene="rpsP"
                     /locus_tag="ACOIAF_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HGZ2669199.1"
                     /translation="MAVKIRLKRMGANKKPFYRIVVADSRAPRDGKFIEEIGYYNPIS
                     EPKQVRINDEKAIKWLATGAQPTEVVKKLLVKNGVIEKFEASKQAK"
     gene            20327..20554
                     /locus_tag="ACOIAF_002191"
     CDS             20327..20554
                     /locus_tag="ACOIAF_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HGZ2669200.1"
                     /translation="MKELVVDIAKALVDNPDSVVVEEFEDNDGIVLKLTVAQEDMGKV
                     IGKQGRIAKAIRTVVRSVANRENIKVSLEIV"
     gene            20662..21177
                     /gene="rimM"
                     /locus_tag="ACOIAF_002192"
     CDS             20662..21177
                     /gene="rimM"
                     /locus_tag="ACOIAF_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438220.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HGZ2669201.1"
                     /translation="MKEKLTHFKVGQIVNTQGLKGEVRVYPLTDDIERFDELKDFYLG
                     KDLNNKLAVEKVRYKGSMVIMKIKGIDSIEKAEKLKNKFMYVAREDSRELEEGEFFIA
                     DMIGMEVLTVDGKYVGILEDVLQYSANDVYVVKGEEDKEFMIPAIKKFVPTIDIDERK
                     MIIDPIKGMID"
     gene            21186..21881
                     /gene="trmD"
                     /locus_tag="ACOIAF_002193"
     CDS             21186..21881
                     /gene="trmD"
                     /locus_tag="ACOIAF_002193"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HGZ2669202.1"
                     /translation="MRFHIMTLFPEIFNSYMDESIMKRAVEKGIIEVHIYNIRDFSNN
                     KHKKVDDYPFGGGAGMVMTPQPIYDTYKHIITTHNINNPSVIYLTPKGKVYNQSMAKQ
                     MSLKEDIILLCGHYEGIDERIIDLIVTDEISIGDYVLTGGELPALIMIDSISRLIPGV
                     LNQEESFEEESFKDNLLEYPHYTRPRDFEGLKVPEVLLSGNHKKIDEWRREESIRITK
                     ERRFDLYKKSNEK"
     gene            22066..22416
                     /gene="rplS"
                     /locus_tag="ACOIAF_002194"
     CDS             22066..22416
                     /gene="rplS"
                     /locus_tag="ACOIAF_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HGZ2669203.1"
                     /translation="MNEMLRAIEQEQLKNEVPNFGPGDTVKVHVRIIEGKRERIQVFE
                     GVVLKRQGGGARETFTVRKMSFNVGVERTFPVHSPKIEKIEVTRKGKVRRAKLNYLRG
                     RVGKAAKIKEARNK"
     gene            22737..23633
                     /gene="ylqF"
                     /locus_tag="ACOIAF_002195"
     CDS             22737..23633
                     /gene="ylqF"
                     /locus_tag="ACOIAF_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HGZ2669204.1"
                     /translation="MSNEYEEYLMDDNLHINWYPGHMKKTKELVKNNLKLIDVVIELL
                     DARIPFSSKNPDIDRLVGDKPRVVVLNKSDMADRDKLNQWIEYYKKINIKAIPVDTIK
                     GVGINKIIEECKNVTREKMSSLKDKGRKERAIRIMIVGVPNVGKSSLINKLTGRKSTQ
                     TGDKPGVTKGKQWVRLKGNLELLDTPGILWPKFEDQEVALNLAFSRAIKDEILDTETL
                     ALRLIEKLMKIEPEKLKARYKLDCLGETPIETMDMIGHKRGFITGKKELDYTRIATTV
                     LNEFRDGKIGNITLEVPENVKR"
BASE COUNT        10131 a         2255 c         4397 g         7075 t
ORIGIN      
        1 ttttttataa tactacaaca tttgaaagtg ttttaaagtt taatatttat ttaaattatt
       61 ataggttaga atggagattt caattatgag aatgtatgat attataaaaa aaaagagaga
      121 taacctagag cttagtaaaa aagagataaa ctactttata gaaaactatt caaagggaga
      181 tatacctgac tatcaagctt ctgcactatt aatgtcgatt tatttaaata agatgaataa
      241 acaggaaact gtacatctta cagaagcaat gatgaactct ggagacatga taaacctatc
      301 tgagatagat ggaataaagg tagataagca tagtactggt ggtgttggag ataagactac
      361 catagctctt atacctttgg ttgcttcatg tggagcacca gttgcaaaga tgtcaggtag
      421 aggtcttggt catactggtg gtactattga taaattggag tctataccag gtttttcgac
      481 agaaatggag ataagtaaat ttattgattc tgtaaataag tctaaaatag cagtttgtgg
      541 tcaaagtgca aaggtagcaa ttgcagataa gaaaatatat gctcttagag atgttacagc
      601 aactgttgat aatatatcat tgatatcatc aagtgtaatg tctaaaaagt tagcttcagg
      661 agctgatgca attgtgctag atgtaaaaac tggtgatgga gcatttatga agacattaga
      721 agagtctttt gagttagcta aatctatggt tgatataggt actggtatga atagagatac
      781 aatagggatt gtaacagata tggatgaacc tttaggtttt gcagtgggaa actctttaga
      841 agttatagaa gcaattgaga cattaaaagg aaatggacct aaagaccttg ttgagctatg
      901 cgaagtatta ggagcatata tgttggtttt atcaaaggta gcacatgatt ttgaagatgg
      961 tgttgaaaaa ataagagaat caataaaatc tggtactgct atacaaaaac tcaaagagtt
     1021 tatagagaat caaggtggta ataaagatgt agtagatgat tattctttat tcaaaaaatc
     1081 aaagtataaa tatccagtta aatcaactaa atcaggttat ataagcaaaa ttaaagctga
     1141 agatgttggt gtatcggcta tgattcttgg agcaggtaga gaaactaaag atgatatttt
     1201 agatttatca gcagggataa ttttagaaaa gaaagtagga gattatgtaa atgaaggtga
     1261 agttttagct tacatgtatt ataatgatga acaaaagttt tcattggcaa agggaagatt
     1321 tattgattcc tactctattg tagattcaaa aatagaaaaa aataaattaa tatatggtat
     1381 tgtaactaaa gaagagatta aaaagtttta gaaaatttta gaaaagtgat tataaaaggc
     1441 ctaacatgtc aaaaataatg ttaggttttt tattttttga taaactataa attataccaa
     1501 aaatatatat tttaaaacat tttttacaat ataaataagt ttattctaca agatgattga
     1561 aaattaaagt atctagtttt ataatagaaa atggataaat atacataagg agtagattat
     1621 attatgaact ttaaagaaaa tagattgaaa atagcagtat taataattgt tattttgatt
     1681 atcttagcag gtatatttgt gtttatacaa ataggtcctt atgataaaaa taacaaaaaa
     1741 gatgtaatta tcgacgttcc aagtggagca tctgttggaa aaatatctga catattatat
     1801 gaaaataaat taataaagaa tgaactatta tttaaacttt tggtaaaggt tagcaataaa
     1861 gctccgtcta taaaatcagg aacatactta cttaatcaat cgtattcaaa caatgatata
     1921 ataagtcttt tagtttcagg taaaatatat caggatggta taaaagtgac tattccagag
     1981 ggagctacat ctaaagaaat tatagcaatg ttagtaagta aaaatttagg agataaagct
     2041 acttttgaaa atttaataaa aaaacctcaa gaattttatg ataaatttcc atatttgaag
     2101 gaagatggaa taacctcttt agaagggttc ttgtatccag aaacttatta ttttaattca
     2161 aagaaacaat cagaagaaga tattttaagc gaaatgttaa aagtatttga cagtaaatac
     2221 acggacaagt ttaaaaagaa acaaaaagaa ttaaatatga ctcttcaaga agtaatggaa
     2281 atggcatcaa taattgagaa agaagctgtt cttgacaaag acagacctat aatagcaagt
     2341 gtgttttata atagacttaa ggtaggtatg cctcttcaat cagatgctac aatacaatat
     2401 atatttgaag agagaaagaa aattgtaaca tatgatgatt taaaaattga ttcaccatat
     2461 aatagttata aaaataaggg attacctcct acaccaatat caaatcctgg tataaaatct
     2521 attgaagcag cattatatcc agagaaaaca gactatttat attttgtagc taagattgac
     2581 ggtggaaata attatagtac aaactatcaa gaccatttaa aatacgttaa agaatataaa
     2641 gaagcaaggg ataaacaatc aaaagataca aaaacaacaa ataaagaaaa tacaaaaaaa
     2701 taaatcatga ttataaaaag tctaacaggg taagaataat gttgggcttt tttattgaaa
     2761 attgttattc ttagatttat aataaagaaa gtgtaaatat atataacaat atgaatttaa
     2821 aaaataataa attaatataa aacttgaata tttattattt tagggagtgt ttttatgagt
     2881 aatatagtta atgatttggt tgaagattat ataagagtga cattaaaaga aaaagaagga
     2941 tttttaaaag atttagaaat ttatgcagag gaaaatagtg ttcctataat acataaagaa
     3001 gtttcagatt tattaaaagt cttgttaaag gttcaaaaac caaagagaat tttagaagta
     3061 ggatgtgcaa ttggttattc ttctattttt tttgcaacta ttataggaaa agatgcagat
     3121 ataataactg ttgagagaaa tgaaaaaatg atagaaaaag caaaagaaaa cataaaatta
     3181 gctggatttg acaataacat aactatacta gaaggagatg cagaagaaaa acttagtcaa
     3241 gtccaaggtg aatttgacct tatatttata gatgcagcca aaggtcaata taagctattt
     3301 tttgatttag ttatagataa attaaaagat gggggacttt tagtatctga taatattttg
     3361 tataaaggta tggttgctca tgatgatttt gttgtaagaa gaaaaaagac tatagttaaa
     3421 agaatgagaa attatttaga ttatatatgt aattgtgact atttaagcac atcattggta
     3481 ccaataggtg atggtgtagc actaagttat aaagaaagta aaagaggtga tgtagatgga
     3541 attaaataaa gtagaacttt tagctccagc aggtgatttg gagagattga aaatagctat
     3601 aacatatgga gcagatgccg tttatatagg tggagaaatt tttggaatga ggtctgcagc
     3661 gaagaatttt agtaaggaag atatggcaga gggagtagct tttgcacatg aaagaggcaa
     3721 gaaagtattc gtaacagtaa atataatacc tcataatgaa gattttctac aattagaaga
     3781 ctatttgttg gaactagaag agataggaat agatgcagtt ataatagctg acccaggagt
     3841 gcttagtgtc attaaaaaag taattccgaa tatggaaata catttaagta cacaagcaaa
     3901 tacaaccaat tatctaagtg caaacttctg gtacgagcat ggaataaaaa gagttgtagt
     3961 ggcaagagaa ttatcttttg atgaaatatc tgagataaga gcaaaaacac ctttagatat
     4021 ggatatagaa gcgtttatgc atggtgctat gtgtatttct tattctggaa gatgtttaat
     4081 aagcaactat atgactggaa gagatgcaaa caaaggttct tgtgctcaat cttgtagatg
     4141 gcaatatcat ttggtggaag aaaaaagacc tggtgagtat tttccaatat acgaagatga
     4201 aaggggtaca ttcttcttta actcaaaaga tttatgtatg atagagtata taccagagtt
     4261 aatcaaatct ggtataacaa gtttaaaaat agaaggtaga atgaagactg catactatgt
     4321 agctactgtt gtaagagcat acagaatggc aatagatgaa ttttacagag acccagaaaa
     4381 ctggaagttc aatccaatgt ggatggaaga actaaaaaaa ggtagtcata gacattttac
     4441 atcaggtttt tacttgaata aaccaacaac agaagaccaa aattatcaat cagcatcata
     4501 tgttagaaac tatgatttta taggtatagt tagagaaaca gaagatgaag atggtcttat
     4561 tgttgttgag caaagaaata aaatgtgtgt tggtgatgaa atagaagtta tgggaccata
     4621 taaagaaact atgtttacta aaattgaaga aatgtacaat gaagaaggag aagctataga
     4681 atcagctcct catcctagac aaatagtgaa gttaaagcta tcagtaaaag ttggtaaaga
     4741 ttatatgctt agaaaagtta tagaagaaaa ggttgaagaa taataaaaaa ttgataaaaa
     4801 tagtattagc cccttagaaa ttgtcaataa tgaaattatc taaggggtga tttcatgtca
     4861 aaaaagaaga ctccattttt gaaaaaagtg ggaaagagaa gctggtgtat atttactata
     4921 attttaataa tttatagtgt tttaatttat aggctagttg atatacaagt attaaaaggt
     4981 gataaataca aacaaagtgt tgaatcacaa agcgttgaga aagtcgagtt aaatagtgga
     5041 agaggaataa tctatgatag aaacaacaag aagcttacag atacaagtaa atctcaggta
     5101 ttaattgttg aaaaagaaaa attaaataat aactataaaa ttttggagct tattaagaaa
     5161 gctactaaga tgaatgattt agatatatat aaagctgtac aagagcaatt gacacgccct
     5221 ataatacaga ttcagactaa aaatatcgat aagagtatga aaaaagaact tgagaaaaat
     5281 ggaataatgg tagaagaaaa aactatgagg tacgcaaaag atggtttatt atcacataca
     5341 ataggatata taaaagaaga tgataaatca ggtcaatcag gaattgaaaa gagtatggat
     5401 agtgtactta gaaattcaaa tgaaaagtat ataagtgctt ttaaagctgg agatgcagga
     5461 aatgaaaaat ctctaaacat actaaaggga agtgtaaaaa ctgttgataa taaggataaa
     5521 gatagacatc taaaaactac tattgattat aacattcaaa aaaaattaga acaaatacta
     5581 aataaagaag aaaatcctac agcagcaata atctcggaag catctacagg tgaaattctt
     5641 gcaatgtgct ctagacctaa ttttgaccaa aatgacatat caaaaagttt aaaaggaaaa
     5701 aatggggaat ttgaaaatag agttataaaa gcgacatatc caccagggtc tgtatttaag
     5761 atggtagttt tattttctgc acttgaaaat ggtgttatag atgagaacta tacttacaac
     5821 tgtacaggta agactaaagt tgggaatact aatgaaatac tgcgatgtaa taaaagagat
     5881 ggtcatggtt tccaaaactt aagacaagct ttttctaatt catgcaatcc tgcattttta
     5941 gatatagcta tgaaacttgg aaaagaaaag atactcaaat ctgctgaaaa attacattta
     6001 tttgaaaagg ttgatattgg tcttgatgaa gaaaaaatta gagaagctcc aaaaaacata
     6061 tcaataagaa atttagctat aggtcaagaa aatatagaat ttacaccact tcaaataaat
     6121 caaatgactc aaataatagc taataatgga acattcaaac cactatactt atataaaagt
     6181 cttgtggaca ataatatgaa tacaataaaa acttataagt cttcaaaaaa agaagaactt
     6241 atttctcctt atgtgtgtac acaagttaaa gaatatatga agtctgtatc taggattggt
     6301 acagccaaag acctaaaaga tattgaaggt ggttgtggag ttaaaactgg tacagctcaa
     6361 agtagtttaa ataaaaaagc tatagaccat ggatggataa caggatttta tcctgaagaa
     6421 agacctaagt atgtaataac tgtgttagta gaaggaactc aaaaaggaaa taaatctgca
     6481 acacctatat ttaaggaaat atgtgaaagt ataaaataaa ttatgtacta taaaatttaa
     6541 ggatatatcg taactttgat tttagataga aaattttgtc taatgatgta cgatatatcc
     6601 ttttaaaata tagtattaac tttcttgaac aagctctttt tctagttttt taagagcttt
     6661 tttttcgatt ctagatacat atgaccttga tatatcgagt ttttgtgcaa tttctctttg
     6721 tgtcttatag ccataaggag ttaacccata tcttaactgg acaatttctc tttctctagg
     6781 agtaagaatt ttgtttagtt gttcgtaaag ttttccaact tgaactttta gttcaacttc
     6841 atcaagaaca tagttagcct ctgtaccaag tatatctaaa agacttattt cattaccttc
     6901 tttatctgtt ccgatagggt cttgtagaga tacttgagtt ttattttttt tgttagatct
     6961 aatattcatt aaaatctcat tttcaataca tctagaagca tatgttgcta atcgagttcc
     7021 tttatctatg ttataagttt ctatagcttt aattaatcct attgtaccta tggatattaa
     7081 atcatcctga tcttccgttg aattattata tttctttgct atataagcta ctaatctcat
     7141 gtttctttca attaatgtat cttttgcact tttatcgttt tctattttaa atttagttaa
     7201 atactcaatc tcttcttcag gggttaaggg tttttcaaaa gatttaagag ctgccaatag
     7261 gtcaccccct tttcaacggt cactttaaat tatatgaagg agacttatcc aagttgactg
     7321 ttatctaata aaataatatg catattttta gaatttaaaa catatatttg caaacttttg
     7381 aatggaatat gacataaaga gtgggaattt aaatttaagt ttttgttgta tatatatgaa
     7441 ataaggcgaa gaaattattg atttataaag tataaattgg aattgaggtg tcaataaata
     7501 taaaaggaaa tagtatttat atatgttttg gtgttgaagt aaaagattaa tgcaaagtat
     7561 tagagtctat aaaaatatga ataaaggagg tgtaatttta caatacttaa aaaagtaaat
     7621 gtaattaatt gtttgtataa agttgattac aagcaaaagt atcagtcata gttaatactg
     7681 ttaaggatga aggtggtgtg ttaatttata tatgtatggg aagaaatttt gattacaatg
     7741 aaaaaactta caaaagatat ttacgaaaaa acttatagtt aaatgtttat atataaaata
     7801 ttatttaggg gttgttttta ttattgaaaa taagaaagag tggttttata tcaattgaat
     7861 gtataataag tattgctata ttatatgtgg ctgtttattt agtttctaca tcattgtata
     7921 attgttatag ttttatcagt agaaatatat ctgacagaga aatgttaagt acagcaaaaa
     7981 aatatataga agatgagaag tatagaatac aaaatagtaa gtatgagtta attaaagata
     8041 agatagaaaa aaattacata aatggatatg aaattaacag tagaatagag caaattttag
     8101 attattatca atgctatgaa ataaatatag agataaaaaa tgaatttaaa aaactgaggt
     8161 ttaatagcta tgttactaga aaataaaggc tctatattaa taataacatt gataatattt
     8221 tctattataa gtactgtgtg tattatgtgt ataggtttaa tttatacaca taataatatg
     8281 tttgagttag gatacaaaga cattcaacta aaagaaattt ctttaagtgg aatagaaata
     8341 tcagtaagta atgtactttc ttgtgttgag gaagctattg atgtatctaa taatgaagac
     8401 gaatttagag aatatttctt aggaaataat atgattgaat gtataaaaaa aataaaagat
     8461 acatcttata gtagtttaga agaaatttct ttaaaagtag aaaataatac tatttatgac
     8521 agaggagatt tttttgaatt tgatatagaa agtaatgtta gagaaaaaga atttttaaaa
     8581 gcaataaaag taagagtgaa aatcaaaaat ccatggatag gaattgacat tgaagattat
     8641 atggataaat ctaataacaa taaaattgat gacaaaacat tcaataatta taaagcatct
     8701 gaaaataatg aaattctaaa tcaatttaaa aatgaaagtg aaaatataag gaatcaagag
     8761 gttgttgaat ttacagaaga agatgaagaa gaacttaaac aaaacataat tgaaaatata
     8821 aagaagaatt ttgatgaaaa aaacctagta caggtatata agtatagggg tgtatagctt
     8881 tgaaaaaaga aggaatgtga ctgtaataga attaatagta tctatcagta taatactctt
     8941 gatagttaca ttatcttttc caaaagataa tcttgaaaat cataaaataa atttatttgc
     9001 acgtcaactt tgttcagata taaggtatgt taggcgaatt aatatgttta gcaactacaa
     9061 tacatatata tattatataa atcaaggtaa acacgaaggt tatgtattga gaaaagatgg
     9121 taaaaacatt aaatcaataa tgctacctaa aaatgcaaaa atagctaaga gtattgaaat
     9181 aataaagttt aaatctgatg ggtcgcctca aaaggcgtct acaatagaga tatataataa
     9241 tagattaaga aaaactataa caataacacc tgtaagtgga agagtacttc tgaaagaggg
     9301 aaaatatgaa acataaatat ggttacttgc ttttggaaag tgtagtatca ttgtcttcaa
     9361 tggtgataat tatactagtt ttatattcta tatttttgtc tacaataaac ttaaagttga
     9421 aagtagaaga taagatagaa ctacaacaac aaagtcttga aatcataaaa tctatggaag
     9481 gaattattag caattctatg ggaataatga atatatctaa ttatgaagaa acatttaaaa
     9541 agacaacatc tataaaatgt agatatgtag atgaaaataa taatgaagaa ggcataagta
     9601 ataaagaaat tatattgaat gaaagacgaa ataagttatt tgtaaattct ttaaatggcg
     9661 aaagtagtca agcaggtggt tatgaaattg gagattatgt agatgaaatg tatgtatcaa
     9721 ttactaataa tggtcaatat gttaatatta aattgaaatt atcaaaacgt agtcaaaaat
     9781 atgagactga tttcaaaata aaagtatgga attttagtga aagtatataa aaatacataa
     9841 atttaagaga aagaaatgac atcaataagt catataaaat ataattattg agaactatat
     9901 aactaaataa tatcaatatg taaagtgtat agattaagaa tatagaggat aataataaaa
     9961 atatagaata agtaatttgc caaatacttg tttatattgt ataacttatg atattatgat
    10021 attagtaaaa atttgtaatc ctgacttgac agaattcaag taagagatat aattttaaag
    10081 gcatagtaat gtaaaattta ctaaaaaata agaaatttat tttaatatgg aggaacaaaa
    10141 aatggtatca gcaggtgatt ttagaaaagg tgttacattt gaaaaagatg gacaaccatg
    10201 tttagtagtt gattttcaac acgttaagcc aggtaaagga gctgctttcg ttagaactaa
    10261 atacaaaaac ttaaaaacag gagctataag agaagaaagt ttcaatccaa gtgaaaaatt
    10321 ccctaaggct gttatagata caagacaaat gcaatatcta tacaatgatg gtgagttata
    10381 ttattttatg gatcaagaaa attttgaaca aataccatta aactatgagc aagttgaaga
    10441 tgcaattaag ttcttaaaag aaaatgaagt tgctacaata agattttatc aaggacaacc
    10501 attccaagtg gaagcgccaa actttgcaga gctagaagtt acagatacag agccaggtat
    10561 aaaaggtgat acagcaagta atgtaactaa agcagctaca gttgaaactg gagcagttgt
    10621 tcaagttcca ttatttataa atactggaga taaagtaaag atagatacta gaacaggtga
    10681 atatttatca agagtataaa aatttatgta tataaaagct tatatgtctt aataatataa
    10741 gatatatgag cttttagttt atatataaaa aagaaagcga ggggtgttac atgaaacatt
    10801 tatatgaaga agttgcatac ttaaaaggcc tagcagaagg actagaaata agtgctgaaa
    10861 gtaaagaagg gaaaatgata cataaaatag ttgatgcatt agaagtattt gcagatgcta
    10921 ttgtaacatt ggatgaagaa caagaagaac ttcaagattt tgttgaatct atagatgaag
    10981 atttagcaga tttagaagaa gatattgctg acatggaaga agatctttat gaagaagatg
    11041 acgatgagga tgatgaggac ttcagctata tagaaatgga atgcccaaat tgtggagagt
    11101 tagtagaaat agacgaagat ttgttatatg atgatgaagt agatgttgtt tgtcctgatt
    11161 gtaaggctgt gatattatca tcagaagatg attgtgatga tgattgtaca tgtggtggtt
    11221 gttcaagttg tgatgataga gagtaaaaat tcataagaat taaaactgcc tatgatgcct
    11281 atgagatatt ttgcttcata ggtagtttta tttttatgag gaagggtatt atgtattttg
    11341 gttttgcaat acttggacaa aaatagtaca ctagaattta tagaatagga ttttggaggg
    11401 atgtaaatat gaaaataagt aaaaaattac tagataatat acaaagattt gaaaatgtta
    11461 taaattataa atttaaaaat aaagaatata tactagaagc tcttactcat agttcatatt
    11521 ctaatgaaaa taaaaaatat aattttaacg aaaggttgga atttttaggg gattcggttt
    11581 taggcatagt gataagtgat tatttattta atgaagaagc taatctgccc gaaggggaac
    11641 taactaaact tagagcaaat atagtttgtg aagattcatt aagtgaagta gctaatgata
    11701 taaatttagg tattcacatg ttattaggta gaggagaaga agcaacagga ggaagacata
    11761 ggacttcaat attagcagac gcatttgaag ctgtaatagc tgctatatat cttgatggag
    11821 gttttgaaag tgcaagacag tttatacttc atcatatgga aaatataatt tatgattcaa
    11881 gaaaaggaaa tatatttaga gactacaaaa cgcatcttca agaggtgtta caagggaatg
    11941 gagaaaataa tatctggtac aggcttatag aagaaaaagg accagaccat aataaaagat
    12001 ttgtaatgga agttggaatt aatgacgatg tacttggtat aggagaaggc aagagtaaaa
    12061 aagaagctga acagttagct gctaaaatag ctttaaagaa aaaattatgg gaaaaataaa
    12121 tataatatgt aaaaatataa aaagaaatat ttatattaga aggatgttgt agatgaaaaa
    12181 gagaattata cctatatttg ttcctcacaa aggatgtcct catgattgca tattttgtaa
    12241 tcaaaaaaaa ataacaggtg tgagtactga tgttactagt gaagatgcaa gaaatataat
    12301 tgaggaatgt ttagagacta tagataaaga tgcagatgta gaaatagcat tttttggtgg
    12361 tagctttact gctatagata ttgatataca aaaaagttta ttatcagttg ctaaagaata
    12421 tgtagaaaaa ggtcttgtaa aagatattag aatgtctaca agaccagatt gtattgatga
    12481 agatatatta agtatgttaa aagaatataa gacaagtata attgaactag gagtacaatc
    12541 cttagatgaa aaagtattat tagatagtat aagaggtcat caatctgaaa ttgtatacaa
    12601 gagttcaaag atgataaaaa atagtggaat caaacttgga ttacagatga tgattggtct
    12661 tccagctgac acagaagaaa agtgtatatt tacagcaaaa aaatttgtag agctaaaacc
    12721 agattgtgtt agagtctatc caactttggt agtaaaagat acaggtcttg aaaagttaat
    12781 ggaacaaaat aaatataatc catttactct tgaagagagt gtaaatatag taaaaaaagt
    12841 tcttgtgctg ttttatgtta ataatatcaa tgtaataaga gttgggcttc aagctactga
    12901 tgatatacaa attggaaaag ctgttttagc aggaccatat catcctgcat ttagagagtt
    12961 ggtagaagca gatatgataa aagattattt agaatttgtt attttgcaaa ataaaaatat
    13021 aaagcaaata ttggtcaaat ctaataaaaa gaatatttct aaaatcatag gaaataaaaa
    13081 aacaaatgtt aaatatatgg aagaaaaatt tggtgtgctt ttaaaaacac aagaatcaga
    13141 tttagatata aatcaattag aaattgtgtt agatgggaaa agtttaatta gtgctaatat
    13201 gagagacatt catagaaaat tgtatgacat ttatgacctt tagttttaat aatgaaggga
    13261 gagaaaagat ttgtatttaa aaagactaga gttaaaggga tttaaatcct ttccagtaaa
    13321 aacagatatt atttttaaag aaggaataac agccatagta ggaccaaatg ggagtggaaa
    13381 aagtaatata tctgatgctg taagatgggt attaggagaa cagagtatta agagtcttag
    13441 aggtgataaa ctcgaagatg taatatttgc aggtacagat acaaaaaaac ctatgaatta
    13501 ttgtgaagtg gctcttacta ttgataattc tgaaaatcaa ttggaattag attttacaga
    13561 agtaactata agaagaaggg cctatagaaa tggtgaaagt gaattttttc taaataataa
    13621 aagttgtaga ttaaaagata taaaagaagt ctttttagat actggtattg gaaaagatgg
    13681 ttattcaatc atagaacaag gaaaagtaga tgaaatatta agtaataatc ctttaagcag
    13741 aagaaaagtt tttgatgaag cttgtggcat atcaaaatat agatataaaa aacaggaagc
    13801 ggaaaggaat ttaagtaata caaaggaaaa tttagaaaga atagatgatg tatatataga
    13861 aattgaaaat caattaaagc ctctttttaa tcaacagaca aaggcaaaaa agtacttgga
    13921 aataagtgaa aaattaaaga cacttgaagt aaatagcttt ataagagaaa ttgaaggaat
    13981 agaaaaagaa ttaagtgagg ttaatgaaca tcgtaaggtt attgaaaagg agctcaatga
    14041 aaaagaagaa caaaaaaatg ttgtagagaa aaagcaagaa gatattaata aagaggtaga
    14101 ggtattacaa gatgtaattg aaaaatctgt agattatata aattcaatta aaggagttat
    14161 atcaaaaaaa gagtcccaaa taaatttaat taaggaaaga ataagaaatt ttacgaatga
    14221 aattagtaga aaaaatttag aaataaatga tataaaagag aagctaaatg aaaataagca
    14281 atacataaaa gaattagaaa gcaataaatt atcaggtagt gaagaactat ctaccttgca
    14341 ggaaaatata aaagtattgg aagcaagtaa agataaacaa aaaattaagt tagaatccct
    14401 aaataatgag atagaattat tgaaagaaag tataattgat atattaaata agaaacaaga
    14461 gtttagtaat aagctgtcca ctttaaacgc aaataaagaa aatatgaata tcagagatga
    14521 aaatataaat tcagagataa cagagttaaa caaaaatatt gaaataaaat catctgaatt
    14581 agatactata aataaagaat ttaatatgca aaatgaaaat ttaaagaatg taaataatag
    14641 acataaagaa ttatcaatta atttgcagga ttcaattagt gaacacaata aactagaaga
    14701 tgaaatacag aaaagtaaat ataatttaaa tggatataat tcaaagttaa atgtctatat
    14761 agatatggaa aatcattatg aaggatttaa tagaggagta aaagaagtac taaaaaataa
    14821 aaatttaaaa ggagttcatg gagcattagg acagattata aatgtacctg aaaagtatga
    14881 gaaatctata gaagcagctc ttggtgctta tatgcaaaat ataataactg ataatgagtt
    14941 tagtgctaag tctgcaataa actatctaaa acagaataat ttaggtagag taacatttct
    15001 tccattaaat ataattaaat caaataaaat aagtctagca aatttaaaag caaatactaa
    15061 gttcatagga atagcaagtg atttaattag atttgatgaa aagtatagaa atataattga
    15121 aaatatactt ggaagaacaa tacttataaa taatatagat gagggcataa aatttgcaaa
    15181 agaaactggt cataggttta aaatagtaac tttagatggt gaaatcttaa atccaggagg
    15241 ttctttgact gggggaagtc taaaaactaa tgggaatata ctatcgagaa aaagatacat
    15301 taatgaatat acagaaaaga taagtaatat caagaatgaa atttcacatc tagaattaaa
    15361 aagagaatct ttagacaaag atgtgaaaaa tatcaaaaat gaaattgatt cacatgaaag
    15421 taaaataaaa gatttagaaa aaagtataat aataaaaagt acaagtataa agaatgttga
    15481 gtctgaaatt gaatcgttaa aaggtagtat tactaaatta gaaaatgaga agaatgattt
    15541 aaattctaac ctaaattata cacttgaaaa aagtgatgat gttagaaaag atatggaaga
    15601 gttagatgat ttatacaata aaaataaaga aaaaattgat gcattgaatg aggaaataaa
    15661 aagatacaat gatttatatg ataaagaaaa atctgaattt gatgaattga acttaagttt
    15721 agtgaagaaa acagaagttt acaatagtat agttagagat ataaaaagaa tatctggtga
    15781 aaattgtgag ctagaagaaa aaaataaaca attagaagag tcattaaatt atgaagaaca
    15841 tgaaataatt aaattacaag attctatact cactgaagaa aaagaaaaag aaaatttaac
    15901 aaaacaacta ggggatagta atagaaatct tgaaactaga aaaatagcaa aagatgattt
    15961 aaaaaatagt tttgatgaaa ttaataaaga attaaaaact atagatagac agcatataga
    16021 acttaaagaa agtttattta aagttggtgg aagattagaa agattaaaaa ctagtcaaga
    16081 cacatatata aataagttgt ttgagcaata tgatatgacc ttagtacaag cattggaaat
    16141 taaagatgaa gatttggata tagatagaaa attcttagaa agtctaaaga gagaaataag
    16201 aagtcttgga aatataaata tagactctat aaaggaatat gaggaaataa aagaaagata
    16261 cgatttttat agcgaacaaa agcaagattt agaagagtct atggaagaga tagaaaaact
    16321 tatacatact ttagaagaaa atatgaaatc tgaatttgaa attaagtttg aagaaataag
    16381 taaaaatttt aaatatgtat ataagagatt atttggtgga ggttgtggag aattaactat
    16441 tttagataaa gaaaatttat tagaaagtga tatactaata acagctcaac ccccaggtaa
    16501 aaaaatgaaa aatttaaacc tattatctgg tggagagaaa gctttaacgg caataagtat
    16561 attatttgct atattgatta caaagccaac accattttgt atattagatg agattgaagc
    16621 gccacttgat gacgcaaata tatttagatt tggagaattt ttgaaggatt tatctaaaga
    16681 gactcaattt atatcagtaa ctcataggag aggaactatg gaagctgctg attatatcta
    16741 cggagttaca atgcaagaaa aagccatttc aaaagttatt agtttaaaat taaaagaagc
    16801 tcaggaaata acagatatta tataacttag attgacatag gaggaataac agtgttaaaa
    16861 aaattatttg gttttggaaa agacaaggaa aaggaaatag aaaaaaaaga tgctgaagaa
    16921 gaaatagaag ttgaagacag tgttgataat ttagaaaact tggaagaaac tattttttca
    16981 ggacttgaag aagaagtgat agataaagtg gaagatattg aagaaaaaaa tgaggaaagt
    17041 gtcaataatg aaggcactga agaaatcgaa aactttgaaa aaatagaagt tgagatggat
    17101 agtaatggaa aagaagtaga aaatatttca aataatgatt ctcaagaaga gataatcgaa
    17161 gaattagaaa attatgatga aattgagtct aaagagatag aagataaaga agataagaaa
    17221 gttaacttgt ttgaaagact aaaacaagga ttgacaaaag caaagcaagg aataactgat
    17281 agaatagatg aagttttaaa atcatataca aaaatagatg aagaattact tgaagattta
    17341 gaggaaattt taataacagc agatgttggt gtaaatacaa ctatggatat aattgaaaga
    17401 ctaagagata aaataaagca aaaaggtatt actgaaccaa taaaggtaag agaagaactt
    17461 aaatctatag ttgaagatat tcttacaaat gaaaactcta cactagatat agaaccagca
    17521 ccatgtataa tattaatggt gggtgtaaat ggggtcggta agactactac aataggtaaa
    17581 ctagcaaata gatacaaaaa agatggtaaa aaagttttat tagcagcagc agatacattt
    17641 agagcagcag caacagagca attagaaatt tgggcaaata gaaccaatgt agacataata
    17701 aaacatcaag agggagcaga ccctggagct gtagtatttg atgctataaa agctgcaaaa
    17761 gctagaaaaa cagatgtatt aatatgtgat acagcaggaa gattacacaa taaggctaat
    17821 cttatgaatg aacttggaaa agtatttaaa atagtagata gagaatttcc agaagcaaaa
    17881 cgtgaagtac ttcttgtggt ggatgctaca acaggtcaaa atgcagttgt tcaagcaaag
    17941 acatttaaag aagttgcaga tataacaggt atagtactta caaaacttga tggaactgca
    18001 aaaggtggag tagtccttgc agtaaaatca gaagtagatg tacctgtaaa acttattgga
    18061 gttggagaaa gtgtagaaga cctacaagat ttcaatgcaa aatcattttc agatgcatta
    18121 tttggtaact aatgttcttt taaaaataat ggttgacaag agtgttgtta tgatataaaa
    18181 tacaatagtg taaagtaaat aacctttaca gtatactgct aggaagtgta agatatgaat
    18241 atagaaaaaa tggtcgaaat tggattgtta tttgaacagt ataaagaatt attaactgac
    18301 aagcagaaag aaatagttgc tctatactat gaagaagatt attctttagg tgaaattagt
    18361 gaaaatctga atgtatcaag acaaggtgtt tatgatacct taaagcgttc agaaaaaata
    18421 ctaagagatt atgaagaaaa gttgcactta gtatcaaaga ttcaagagca ggaaaaaaat
    18481 attaagatta taaaagataa aattattgac attaaagaag atttattgca caatagagat
    18541 tgtgctaatt taatccctaa gctagaaaat atagaagatg tatgtaggga gatgataaaa
    18601 tgatatttga aggattgtct gataaactac aaggggcatt tagtaagtta aaatctaaag
    18661 gaaaacttac agaagtagat gtaaaaaatg ctatgagaga agttaaactt gctcttcttg
    18721 aggcagatgt taactttaaa gtagttaaag atttcataaa aaaagttcaa gagagatgtg
    18781 ttggacaaga agttatgcaa agcttaactc cagctcagca tgtaatcaaa attgtaaatg
    18841 aagagcttac aagtttaatg ggagatgtac aaagtaaagt aatgatttcc tcaaagcctc
    18901 ctacaatttt aatgatggtt ggattgcaag gtgcaggtaa gactactaca gcaggaaaac
    18961 ttggaggata ttttaaaaag caaggtaaaa aacctctatt agtagcttgt gatatttata
    19021 gaccagcagc tataaagcaa cttcaagttg ttggagaaaa attggatata cctgtattca
    19081 atatgggaga taaagaaaat ccagtaaata ttgcgaaagc aggtttaagc catgcgatta
    19141 aaaatgctaa tgatttagtt attatagata cagctggtag acttcatata gatgaagtac
    19201 ttatggatga gttaaagtct attaaatcag aagttaagcc acatgagata cttttggttg
    19261 tagattctat gacaggtcaa gatgctgtaa atgttgcaga aagcttcaat gaggcacttg
    19321 gtgttgatgg agtagtatta actaaattgg atggagatac tagaggtggt gctgcacttt
    19381 caataagagc agttacacaa aagcctataa aatttatggg gatgggagaa aagctagatg
    19441 atatagaacc tttccaccca gatagaatgg catcaagaat attaggtatg ggagatgtct
    19501 taagtttaat agaaaaagca caagaaaata tagacttaga gaaagctaaa gaattagagc
    19561 aaaaaattaa aaaacaagag cttgattttg aagatttctt acaacaaatg gaacaaatac
    19621 aaaatatggg acctcttgac aagatacttg gtatgatacc tggtatggga aatgtaaaag
    19681 accaacttgg agatgtagat ttgaatggca aagaaatgaa gagaacaaaa gccataattc
    19741 agtcaatgac tacatatgag agaagaaacc caggagtatt aaatgcttca agaaagaaaa
    19801 gaatagctaa aggcagtgga actagtgtac aagatgtaaa tagacttata aaacaactta
    19861 atgaaatgaa aaaaatgatg aagatgtttg ctggttcaca aaagagtatg aagaaaaggg
    19921 gcggcttagg cggtttacct ttttttaaat aaaatataaa aaagtaaaaa ttattcggag
    19981 gtgactgtaa tggcagttaa aataagatta aaaagaatgg gagctaacaa aaaacctttc
    20041 tacagaatag tagtagctga ttcaagagct ccaagagatg gaaaatttat agaagaaata
    20101 ggatactata atccaatttc tgaacctaaa caagtaagaa taaatgatga aaaagctata
    20161 aaatggttag ctactggtgc tcagccaaca gaagtagtta aaaaattact tgtaaaaaac
    20221 ggagtaatag aaaaattcga agcttctaaa caagcaaaat aattttttct tggcgattag
    20281 tttaagtaag ggctcattgc taaggttact ttaggagatg aaatatatga aagagctagt
    20341 tgtagatata gctaaggctc tagttgataa tcctgactca gttgttgttg aggaatttga
    20401 agataatgat ggtatcgtct taaagcttac agttgctcaa gaggatatgg gtaaggttat
    20461 tgggaagcag ggaagaatag ctaaagctat aagaactgtc gtaagatctg ttgcaaatag
    20521 agaaaatata aaagtttctc ttgagatagt ataaaatagg aggattaggt ataatgccta
    20581 gtccttttta tataaatgcg tagtttaaga ttttatgatg atatgaccac ataagattaa
    20641 attttttaag aaggtgataa catgaaagaa aaattaactc attttaaggt aggtcaaata
    20701 gtaaatacac aaggtttaaa aggtgaagta agagtttatc cgcttactga tgatatagag
    20761 aggtttgatg aattaaaaga tttctatctg ggaaaagatt taaataataa attggctgtt
    20821 gaaaaagtaa gatacaaagg ttctatggtt ataatgaaga taaaaggcat agacagcata
    20881 gagaaagcag aaaaactaaa aaataaattt atgtatgtag ctagagaaga tagcagagag
    20941 cttgaagaag gcgaattttt tatagcagat atgattggaa tggaagtttt aactgtagat
    21001 ggaaaatatg tagggatact agaagatgtt cttcaatact cagcaaatga tgtctatgta
    21061 gtaaaaggtg aggaagataa agaatttatg ataccagcta taaaaaaatt tgtacctaca
    21121 atagatattg atgaaagaaa aatgattata gaccctatta agggaatgat agattaggtg
    21181 gtaatatgag atttcatata atgacacttt ttccagaaat atttaattcg tacatggatg
    21241 aaagtataat gaagagagca gttgaaaaag gtataattga ggttcatata tataatataa
    21301 gggatttttc taataataaa cataaaaaag tagatgatta tccatttgga ggaggagcgg
    21361 ggatggtaat gactcctcaa cctatatatg acacatataa gcatataata acaactcata
    21421 atataaataa cccaagtgta atttatctta ctccaaaagg taaggtatac aatcaatcta
    21481 tggcaaagca gatgtcttta aaagaagata taatactact atgtggtcat tatgaaggaa
    21541 tagatgagag aatcattgat ttaatagtta ctgatgaaat ttcaattgga gattatgtat
    21601 taactggggg agagctccca gcacttataa tgatagattc tatatcgaga ttaataccag
    21661 gtgtattaaa ccaagaagaa tcatttgaag aagaatcatt taaagacaac ttattggaat
    21721 atcctcatta cactagacct agagattttg aaggattaaa agtaccagaa gtgcttttat
    21781 cagggaatca taagaagata gatgagtgga gaagagaaga atctataagg ataaccaaag
    21841 aaagacgatt tgatttatat aaaaagtcta atgaaaaata aaaatataat gaaaaattaa
    21901 tatatatgta tacaataaag tacctataaa tggaattaaa aacataaaag tgcttgtaat
    21961 aactattgaa gtatagtata atataaaatg ttgaaaatac gggcgttcct ctgtttacac
    22021 tggtcgggta gttaagaacg tctatgatat ctaggaggaa gaaagatgaa tgaaatgcta
    22081 agagcaattg agcaagaaca attaaaaaat gaagttccta attttggacc tggggacaca
    22141 gttaaagtac acgttagaat tatagaagga aaaagagaaa gaatacaagt attcgaaggt
    22201 gtagtattaa agagacaagg tggaggagca agagaaactt tcacagttag aaaaatgtct
    22261 ttcaatgttg gtgtagaaag aactttccca gttcactctc caaaaataga aaaaatagaa
    22321 gttactagaa aaggtaaagt aagaagagct aaactaaact acttaagagg tagagtaggt
    22381 aaagctgcta agataaaaga agctagaaat aaataattaa aaactttact tataaatggg
    22441 gggctgattt atcagtcctc tttctatttt aatgaaaatt acaacaaata attatactag
    22501 aaataaatag aaatatttgt tgaaaatata gattatttat ctatgaattt atagctttta
    22561 aaatgaaaaa taaaattggt aaatattgaa ttatattttg taacgatatt aaaaataaga
    22621 gaacagtatt atgtaaagta tgacgttaaa aagatgacaa tattatagtt tgtgaagata
    22681 tattttttaa gtggaattag gatataataa tataatatta ataaggagtg atacatgtgt
    22741 caaatgaata cgaagagtat cttatggatg acaatctaca tataaactgg tatccaggtc
    22801 atatgaaaaa aactaaggag ttagtaaaaa ataacttaaa attgattgat gtagtaattg
    22861 aacttttgga tgctagaatc ccgtttagta gtaaaaatcc agacatagac aggcttgtag
    22921 gagataagcc aagagttgtc gtacttaata agagtgatat ggcagatagg gataaattga
    22981 atcaatggat tgaatactat aagaaaatta atatcaaagc tataccagta gatacaataa
    23041 aaggtgttgg tataaataag ataatcgaag aatgtaaaaa tgtaactaga gaaaaaatga
    23101 gttctttaaa agacaaaggg agaaaagaaa gagctatacg aataatgata gttggagttc
    23161 caaatgttgg taaatcgtct cttataaaca aattaacagg tagaaaaagc actcaaactg
    23221 gagataagcc aggagtaacg aaagggaaac aatgggttag attaaaggga aatcttgaac
    23281 ttttagatac gcctggaata ctttggccta agtttgaaga ccaagaagtt gcattaaatt
    23341 tagcatttag tagagctata aaggatgaga ttttagatac tgaaactttg gctttaagac
    23401 ttatagaaaa acttatgaaa atagaacctg agaagttgaa agccagatat aagttagatt
    23461 gtctaggaga aactccaatt gaaactatgg atatgattgg tcataaaaga gggtttataa
    23521 caggtaaaaa agaactagat tatacacgca tagccacaac tgtattaaat gaatttagag
    23581 atggaaaaat aggaaatata acattggaag ttccagaaaa cgttaaaaga tagattttaa
    23641 aacaaaatca aaaatttaat gttaaaaatt taaaaaagcc ttttagcaaa ggctttttta
    23701 tttttttgaa atatagacaa atatctccat ataaaagtat aaattaggtt agaaaacctt
    23761 taaaaatatt aatttaagac aaatatacct aatattttga aaattaaaat gagtgtgatt
    23821 atttaaaatt ttagcatatt gacttgcttg tttcgtat
//
LOCUS       DBGJFF010000057        23518 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.058, whole genome shotgun sequence.
ACCESSION   DBGJFF010000057 DBGJFF010000000
VERSION     DBGJFF010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23518
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.058"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            262..2280
                     /locus_tag="ACOIAF_002196"
     CDS             262..2280
                     /locus_tag="ACOIAF_002196"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HGZ2669205.1"
                     /translation="MRRNTKLLTTGILSMAIVTPTMAFATESNAMENNADLNINLEKK
                     SIVLGSTSKVSVKFKEKPDADSITLKYKCYDMPLDTTLNYNQSTESYEGTINYNKDPE
                     YLNVWELQGITINSKNNPKTLNKQELEKMGLNLKDYNVTQECIIEDITSRKDVNKYLR
                     KTSAPITELTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGIISTPLATTYNAPI
                     LLVEKNNVPNSVKSELKRLNPRDVIIIGDENAISKTTANQIKSTVNASQTRLKGSNRY
                     ETSLLIAKEIDKNHDVEKVYITNANGGEVDALTIAAKAGQDKQPIILTDKNSITDNTY
                     KWLKSEDLQNAYFIGGPQMISTNVINKVNDITKDNVTNNRVYGADRHETNANVIKKFY
                     TDDELEAVLVAKSDVLVDALAAGPLAANLKSPILITPKTYVSAYHKDNLEAKSANKVY
                     KIGGGLTSKVMNSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGGSDTGTTGKPLGGIK
                     EKDYTLNTSLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNSLRPDLFTSIHYNAS
                     DTTGNGVEVFYKLKDKDGGTTKTVATNILNRILEKFNLKNRGAKTRTLSTDPTKDYLY
                     VLRNNDMPAVLVECAFLDNEKDMSLLNTSNKVKEMGTQIGKGIEDSLK"
     gene            2785..4797
                     /locus_tag="ACOIAF_002197"
     CDS             2785..4797
                     /locus_tag="ACOIAF_002197"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HGZ2669206.1"
                     /translation="MRRNTKLLTTGILSMAIVAPTMAFATESNAMENNADLNINLEKK
                     SIVLGSKSKVSVKFKEKPDADSITLKYKCYDMPLNTTLNYNQSTGAYEGTINYNQDPE
                     YLNVWELQGITINSKNNHKTLNRQDLEKLGLNLKDYNVTQECIIEDITSRKDVNKYLR
                     KTSSPITELTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGIISTPLATTYNAPI
                     LLVEKNNVPNSVKSELKRLNPKDIIIIGDENAISKTTANQIKSTVNASQTRLNGSNRY
                     ETSLLIAKEIDKNHDVEKVYITNANGGEVDALTIAAKAGQDKQPIILTDKDSITDNTY
                     KWLKSEDLQNAYFIGGPQMISTNVINKVNGITKDSVTNNRVYGADRHETNANVIKKFY
                     TDDELEAVLVAKSDVLVDALAAGPLAANLKSPILITPKTYVSAYHKDNLEAKSANKVY
                     KIGGGLTSKVMSSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGGSAPGNSSGGMIEKD
                     YNLNTSLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNSLKPDLFTSIHYNGSTNK
                     QGHGVEVFYKLKDKNGGTTKTVATNILNRILEKFKLTNRGIKTRVLPSDSTKDYLYVL
                     RSNDMPAVLVECAFLDNENDMSLINSSAKVKEMGTQIGKGIEDSLK"
     gene            5039..6952
                     /locus_tag="ACOIAF_002198"
     CDS             5039..6952
                     /locus_tag="ACOIAF_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669207.1"
                     /translation="MEYLFEKSSLKEYGNEALDKYRELLDKTGNNSRCILLLVPNNNT
                     KIRYERALRLNYSEELKITTYISFVKKELVKYWPLIIEKCDEISTKVVSPTFISNSLS
                     EYLIVQKVKEKRIQEGYFGDITGTNKSIANSIMTNINKSAFNLIDFDFIGEKIYSSKK
                     NKDSIYRFSYTQMDEIISYYINSLLSNSILDNALSIYLYNQYLLNDDFYLKNLFTEVR
                     YIIVDSLESSSNAEVDFITEALDNTLQSYIYFDYSRDYSVFNNVDIDYINEKIISKIK
                     PKEENIKKKEIRIEDLYLLPANIELNQSSQLYNEMLDLISEKVISLIEEDVSPRDIAI
                     ISPINNSILEHQIRDSLIEKNIDVFSTKKDKKAVDYPYGNALVVATCIFYGYLDFIKD
                     DEYLSFLETLLEINRIKAFKIFNETRHLSIDNIDEHSQYRDILQYIEEKKKSDIKIHE
                     FLTQFYIDKMLNLKEGKKNVGLCKKIIAESESFSESLSLLGMKKEKIFIEALKSTIND
                     YYSVVDIEELKSKDKIVITTPYSYISSNIDRSIQIWVDIGSNAWNMKIEKDISNLIVL
                     RKSFEEKKIYTNEMEEYYKKYYLYNTVYNLLLNAKKIYAFKSEYAINGYIQESILYSI
                     LLKISHKGDKNYD"
     gene            6945..9164
                     /locus_tag="ACOIAF_002199"
     CDS             6945..9164
                     /locus_tag="ACOIAF_002199"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HGZ2669208.1"
                     /translation="MININYREDQIPIINYDNGTMAVPAVPGAGKTFIITNLVAKLLL
                     EQKHKGGKILILTYMNSAVNNFKGRIRKILEENKIDDTNSYEVMTIHSLAVKIIKEKP
                     EVVMLSEDFNIADDLQKSIILNECISRFRFEGGERAFRWFLKEQKDERWKEITLEAWE
                     RGFFEFAGNAISELKYKGISPDKLEEILSRGHKGMLKIILPIYKLYDRKLKQNGLLDY
                     DDILILAEKTLSLDEGLRKKFQTRYKYIFEDECQDSNEIQGNIIKIISSENKNLVRVG
                     DINQSITGTFSSSDPKFFKEFINSADFCYRMDMSNRSSKDILDLANTLVKYVTKEFRQ
                     KECRHALEDMQIKTVPNGMGYKENPNPEHYNINVKWYESWKIEIEQTARYVKGIKNKY
                     PDKSIGILVPFNEQVTQVAKELRENNLTFEELGPNSLNKRKVINNIAYIIDFMLHCDD
                     VEKLIVVLNKVFIDTDNEEEIKYFLEELKNYSTEEIIYNFEFSKLDNDLIEFKEESDI
                     YLSFKRGIDIIKQILEYPIIRLDLLVLFIGKKLNLEKEDKAVLDYISFYIKYLVSENI
                     NMDLEDVYNLLFDVKNRVFNHIIDVVYEINGYEPEPGSITLCNYHKSKGMEWDCVFLL
                     GLVEYNFPDNINQKFQSDKWYLKEKYKNPMAIIKSEVEAILKGSISTDYAHKTKIDSI
                     NEKIRLLYVGITRAKEMLVLSGSAYRDESDIGNKRKEQKPCIYLSRLNQHIIEKRSN"
     gene            9273..10103
                     /locus_tag="ACOIAF_002200"
     CDS             9273..10103
                     /locus_tag="ACOIAF_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HGZ2669209.1"
                     /translation="MNDKLKYFSYSQNSINTYKSCPLKFKYKYIDRINWKNDDVGSRE
                     YYETLKTGRDFHLICERYFSNIPLGIYFNEDDKNSKKFLKWMENIKKVVPIEKGKTYL
                     PEYEVRMTLNGDIIQAKYDLVVIGNNNIEIWDWKTESKKIDYKHVENRIQTIVYMFLA
                     KEVIPKVLKMDINVKDINMRYYQPEFDDLPITILYDEEKHEANRNKIQNYISMIKNTN
                     YEEHTYEDNLYNDIEKVYNKEERMCYRNKKHCKYCEFNKLCNGKEIDYSILEAEIYGT
                     "
     gene            10093..13560
                     /gene="addB"
                     /locus_tag="ACOIAF_002201"
     CDS             10093..13560
                     /gene="addB"
                     /locus_tag="ACOIAF_002201"
                     /EC_number="3.1.-.-"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HGZ2669210.1"
                     /translation="MGLRFVLGRSGSGKSTYILDEIKKEAQKNETTSIILLVPEQYTF
                     EAENRVSKLFLGKEKDKYLRVRVLSFKTLSNIVFSQVGGLTDVNINSSGKAMMVYRAI
                     EDVSEELNVFSKSKSQSGFVSSITDMISEMKQYNISPEMLENISGELDNETLSLKLKD
                     ISKIYNSFEGKLHENYVDAQDMLTSLASKIELSSYLDGACVYIDEFTGFTPNQYNVIK
                     SILNKSKSVNISLTVDDINYIGYSKSDMFSRTKFTYSKLTQLCNEEGIKILPQVNLNT
                     GVIKRFEKVKELQHLERFYNAYPYKIYSNPTENIKIKEFNNLYSEVEEIAREIVHLVR
                     DKNVRYRDITIATRDLNRYDFLVHSIFNEYNIPNFIDKKREAKSNPIVILIISTLEMK
                     NRRYGYETMFRYLKSGLIGIDNDDINLLENYVLANGIKGKKWFDEKWDYRITQSLSGQ
                     ESEFELELKEKINEIKNRVLEPIVILQEKLRGKNRVKEICRYIYEFLLDINMPETIES
                     LIVNFKDKGELDVANQYSQVWDIVVDILDQMVELMGDEIISLEKFIKLITLGFDEYEL
                     GLVPPSIDQVLVSSVDRMKNPDTKYLYLVGTTDGVFPLITKDSGILSDNDRESLGNKG
                     IEVDIDSKTRSFEEQFLVYKALTSTSKNLTITYPISDHEGKTLRPSIIISRLKKIFPN
                     IENKSYLVEENKSTDKDILKKITVKSPTFNELINIIKNYDSDGYNTEEINSIWLDIYR
                     YYLKDEVYSSITKKVIKGLSYTNQVHKIEEKKIRSLYKSNSLSISRLEKYAECPFAYF
                     IQYGLKAKKRKEYSFTPPDLGTFIHNILDRFSKELLQDNLTWRDIDEKYIELKIGIIV
                     DEIILKIPGYILNSSERYKYLAYRLKNMLTTAITIISQQIKQGSFEPIDYEVKFGNNG
                     KYPPIKMVLENGQEVSLIGQIDRVDEFEEGENKYIRIIDYKSGNKSISLTEIYYGLQL
                     QLLVYLDAILESAKDEDININPAAILYCRINNPIAKFNEDKDDEEIQEAILKELRMKG
                     LVVKDSHIVKEMDKSLIDGERKNSLVIPVGLTKDGNVGKSTSAISYEDFKLLRKYVRH
                     AIKDLCEEMLSGEIRIAPYKHKDGTSCDFCDYSAICQFDSTIKDNKYKNLNNKSNEEI
                     IKMMKGDVN"
     gene            13560..17387
                     /gene="addA"
                     /locus_tag="ACOIAF_002202"
     CDS             13560..17387
                     /gene="addA"
                     /locus_tag="ACOIAF_002202"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HGZ2669211.1"
                     /translation="MSSPKWTKEQLEVIESRECNLLVAAAAGSGKTAVLVERIIQMIT
                     SRENPIDIDKLLVVTFTNAAASEMRERIGDAIGKALDENPENKHLQNQLVLLNKSSIT
                     TIHSFCLDVIKSNFHRINLDPNFRIGDQTECAILKQEAIEEVFEDLYEERDEGFLNLV
                     ESYAERGGDKEVQDIILGIYSFAMASPEPKKWLIDSAERFNIDENFDFSQSIWARAIL
                     DTVKIEINGLCLNMERALKEVESIEELETFAEKLSVEYKKIADISQACNKSWDEAYKK
                     MASMSFENYVKGVKRISKDAPSYIKESKEKAKTIRDKTKKSLESIVSATFNKDNDSIR
                     EEIKYLYNIVKPISSVVLRFEEEYSNKKREKGIIDFNDIEHFALNILTDVDEKGNIVP
                     SDIAVGYRNKFYEIFIDEYQDSNLVQEVLLKAVANTETPNRFMVGDVKQSIYRFRQAK
                     PELFLQKYNNYNDKKGSSHRKIMLYKNFRSREEVVDAVNYIFENIMNENIGEIEYTEK
                     ERLNLGANFNVDTDEKSIIGGATEIHLIQKDNKLDDDIINDKDDRINNKEDEIEEEEK
                     LDNIQLEARMVGNIIKDLMKVNEDGKIQKVYDKGIDGYRPVEFRDIVILLRATSAWAP
                     VFADELMNMDIPTYADVGVGYFDTIEIKTILSLLQIIDNPMQDIPLISVLKSPIFGFT
                     PEDLIDIRVQSKDKIFYEVLKSTAEYDGFTDSQNENESEFIPSEECINKSKDFLIKLK
                     EFKEKSMYMSTDEFIWYLYTRTGYYAYVGALPGGSQRQANLKVLFERAKQFEETSLKG
                     IFNFVNFIEKLKKSSSDMGSAKTLGENANVVRIMSIHKSKGLEFPVVICSAMGKNFNT
                     QDFKKSILYHHNLGYGPQFVDYERRISFPSIAKEALKSKINIENLSEEMRVLYVAFTR
                     AKEKLIITGSTRNIQDSIKRWSNGIESLDTISQYEILKGKNFLDWIMPCVLRHRDLSN
                     LLEEVGLDAVFNVEHNSKWYGKLWNKNDILVEKKSDEEKESIEEILEKIDVDNPDSDY
                     YSEIEEKLNYIYPYEFSTRKPATISVTEIKKIQNNYEEELINTIFEQKVILKKPLFIQ
                     NEEEREKISGTERGTIVHLVMEVLDLKNVSSVNDIKSQIRGFVSKGIITEKQASIVNP
                     YKIYKFFASNIGKRMLNAEIINREKSIYAQVNMKDIYIYEKLINNDDKKLYDNESVML
                     RGIVDAYFEEDNQIVLVDYKTDFVNEENINQIIEKYKKQLDLYADIIETLTGKSVKEK
                     CIYLFGVDEAVCY"
     gene            17433..18644
                     /locus_tag="ACOIAF_002203"
     CDS             17433..18644
                     /locus_tag="ACOIAF_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HGZ2669212.1"
                     /translation="MRFIHTSDWHLGKSLEGHSRIEEQAKFCEEFIKIVESNEIDMVI
                     IAGDVYDTSNPPAQAEKLFYQTVSRLANNGQRCVLIISGNHDNPERLSAITPLAHEQG
                     ILIYGYPLSATIEAKYKGFEITYATQGCTKLNINGENIVVATLPYPSEKRLNEVFSSE
                     DEFEKQKNYSEKVGDIFRSLEENFRSDTINIAVSHIFVIGGESTESERPIQLGGSFLV
                     ERKDLPEKAQYTALGHLHKQQKASERLNAYYSGSPLQYSKDERAYTKGAYIVDIKAGE
                     KPIIEDVYFNNYKPIEVFKCNGIEEALDICEQNQDREIWSYFEINTDEIISQNEIKKM
                     KELLKDIIEIKPIITSCYEQESVDIKEKSMAELFREFYSFSKGVEPKGELMDLFLDII
                     SEEGESADETN"
     gene            18631..22158
                     /locus_tag="ACOIAF_002204"
     CDS             18631..22158
                     /locus_tag="ACOIAF_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HGZ2669213.1"
                     /translation="MRPIRLELTGLNSYIDKQVIDFEKLIERGLFGIFGTTGSGKSTI
                     LDAITIAMYGNISRNTKEYINSVCDKAIISYEFEIGSKNTRRRYIVDRTIARSKTGTK
                     TSNARLIEVLNDNTQNVLADKVVEVNEKVAQVVGLTANDFTRSVVLPQDKFNEFLRLS
                     RADRRDMLERIFNLEKYGRSLGEKVKKRKNIQLQNLKDLKSKLSQYDGITEEVYNNIN
                     QELIELKNLEKDKNNALDLAQKSYEESKTVYEEQLKLEKNELRKNELDLKNSEIKEKI
                     ALVENDSNAKKVDPYISSVQNLEKKIDEDSFAVSNLEKKLAILNQELEVTKNRYEKIN
                     KIKNEEVPKLSEEKIRLQQAIKLEEELVLLDRELKELKESGINLNKTKVELEKVKQVS
                     ESRKDAVTKSIKEVEGKIDKVNISAELKQKIFLAYEYEKDYNNVLEEKNQKLNKLEEI
                     LKETENINLKVRYIDKDKNDVNRNLENLSLHLDVLLKKCPGKSADLLLKSEYVTELRN
                     KANNTKENEIKKSSIQDELKIILESKFNTEREVNLLNEKLENNRKNRDDLEKELEELK
                     YLNLASELRRELKENMPCPVCGSKHHENHNITKYDENISFVKEKLEKLEKEKISIRNN
                     IEELNAKVSGYLSIEKMKAKELEDVKGKLGGIPSSQLLKKLDEEQRKLALLKSNIQEW
                     EKEKESTENKVTLAKEEKNKIEKEELKIRESLNNYKKLTRDLNIEIEGLENKCKKLKQ
                     EYLGLKTITKVSNLLSKVKEIRENEKELELLSTGHSNLLKNRDSLDIKIRENESKLHE
                     IEIELIKARELYVEKKLSRDNKYKEVISITKGDLAKNLLHNVEENICKILEQEESSKK
                     KLEEQRLEYEKNIAEKHNIDGRLKTAKEQYKDQKSTLNKLLAENKFESIYAVKRALLD
                     TDAVKKLIEEILEHEEEQKLLSFKIKSSKEKLNGRSIKKEYFEQLKDEIYNLKVEIGN
                     ISKDIGANQNQLITLKDSLDKINDFNKQLKVVEHNVDLLEELDKCVQGNKFVEYVSTN
                     QLKYIALEASKRLDGITKGRYALEIDSTLNFVMRDNFNGGERRSIDTLSGGETFLTSL
                     SLALALSSQIQLKGSAPLEFFFLDEGFGSLDSELLEIVIESLERLHSNNLSVGIISHV
                     EELKNRVPVKLLVSSSEAGIGSKVKIEYS"
     gene            22289..>23518
                     /locus_tag="ACOIAF_002205"
     CDS             22289..>23518
                     /locus_tag="ACOIAF_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HGZ2669214.1"
                     /translation="MLESPNNLIQIIFLIVLLIGSAFFSASETALMSLSKIRIRYMQD
                     EGVKGAKLVSSLIENPNKLLSSILVGNNVVNIAATSISTSLFIGLMGEKGVALATAVM
                     TVLVLIFGEITPKTIAANNSEKVSLLVSKPIKAIIFILRPIVWIFNIITNIIFKLFGI
                     TNKGAKSFITEEELKTMVNVSHEEGVLEMEEREIINNVFEFGDMQAKNAMVQRIDMVA
                     IDMEDSYDEIIQVFKTEKLSRMPVYEETIDDIVGILNIKDIIFLSDEEIESFDIKNYM
                     REPFFTYEFKKITQLLEEMKLEKSQMAIVVDEYGGTSGLLTIEDLVEVIVGDIEDEYD
                     EEEDEIQVIKEDEYIVDGSTKIGDVNELIGVNLESEEFDSIGGFIIGHLSRLPEENEV
                     IEVDNIRFCIESIEKNRI"
BASE COUNT        10142 a         2269 c         4134 g         6973 t
ORIGIN      
        1 ttgtattaaa tttaataaac ctacaattat tatatgtttt tatttgtctt ttatagattt
       61 ttttggcaat aaatgatata gtgaagataa ttaaatatat tcaatatatt taattttttt
      121 attttttgtt tttataattt ttttagaagt ttatttttta tgtacttatt tataattttg
      181 ggtaaaaaca ctttgatata ttttcaaaag ataaaagatt aaaaaacaag gtagaaattt
      241 atgttaagta aggagattaa tatgagaaga aatacaaaat tattaacaac aggaattctt
      301 tcaatggcaa tcgtcacacc tacaatggca tttgctactg aatctaatgc tatggaaaat
      361 aacgctgatt taaatataaa cttagagaaa aaaagtatcg ttttaggtag tacctcaaaa
      421 gtcagtgtta agtttaaaga aaaaccagat gcagatagca ttacattaaa gtataaatgc
      481 tatgatatgc cattggatac aactttaaat tataatcaat caactgagtc atatgaagga
      541 actatcaatt ataataaaga cccagaatat ctaaatgttt gggaactaca agggataaca
      601 ataaacagca aaaataatcc taaaacttta aacaaacaag aactagaaaa gatgggatta
      661 aatttaaaag actataatgt aacgcaggaa tgtataattg aagatataac ttctagaaaa
      721 gatgtaaata aatatttaag aaaaacttct gcacctatta cagaacttac aggaagtgat
      781 agatacgaaa cagcagttaa aataagtaaa gaaggctgga aaaatggttc agataaggta
      841 gttataataa atggagatgt aagtatagat ggcattatat caactccact agcaactaca
      901 tataatgcgc caatactttt ggttgaaaaa aacaatgtac ctaatagtgt aaaatcagaa
      961 ttaaagcgtt taaatcctag agatgtaatt ataattggag atgagaatgc tatttctaaa
     1021 actactgcta atcaaattaa atcgactgta aatgctagtc aaacacgtct aaagggctct
     1081 aatagatatg agacatcttt attgatagca aaggaaatag ataaaaatca tgatgtggaa
     1141 aaagtataca taacaaatgc taatggcgga gaagtggatg cacttactat agcagcaaaa
     1201 gcaggtcaag acaagcaacc aattatatta actgataaaa atagtattac agacaacaca
     1261 tataaatggt taaagagtga ggatttacaa aatgcttatt ttataggtgg tcctcaaatg
     1321 atatcaacaa atgttataaa taaggtaaat gatataacta aagataatgt tactaataat
     1381 agagtatatg gagcagatag acacgaaaca aatgcaaacg taataaaaaa attctataca
     1441 gatgatgagt tagaggctgt tttagtagct aaatcagatg tacttgttga tgctttagca
     1501 gcaggtccat tggctgccaa cttaaaatct ccaatactta taacaccaaa gacgtatgta
     1561 tctgcatacc ataaagataa tttagaagct aaatcagcta ataaagtata taaaatagga
     1621 ggaggattga cttctaaggt aatgaactct atagcatcat cattatctaa acacaataca
     1681 actccaacag aaccaggaaa tagtgggggt aagacagtta tgattgaccc aggacatggt
     1741 ggttcggaca ctggaacaac aggtaaacca ttagggggta ttaaagaaaa agactataca
     1801 ttaaatactt cacttgcgac aactgaatat ttacgttcaa agggattcaa tgtaataatg
     1861 acaagagaca cagataagac tttatctctt ggaaatagaa ctgctttatc taactctttg
     1921 agaccagatt tatttacgag tatacactat aatgcatctg atacaactgg gaatggtgta
     1981 gaagtatttt ataagcttaa agataaagat ggagggacta ctaaaactgt agctacaaat
     2041 atattaaata gaatcttaga gaaatttaat ctaaaaaata gaggtgcaaa aacaagaaca
     2101 ctatcaactg accctacaaa agattattta tatgttttaa gaaataatga tatgccagct
     2161 gtacttgtag aatgtgcatt tttggataat gagaaagata tgagtttact taatacatca
     2221 aataaagtaa aagaaatggg tacacaaata ggtaaaggca tagaagattc attaaaatag
     2281 tctacaatga gagcatagaa aaatttaaaa taaattaatc tgcaatttta aatttaaact
     2341 aaagctataa gataagaatg taattataat agtaataaag actgtatata cattgtgcat
     2401 ataagtgcga aatgaggtat atacagtctt tttatgatta attgtaaaag gatttatatt
     2461 atatgaagaa tttacatatc ttaaggctac agttaattgt caagagttgg agtattcatt
     2521 tgtaatacac aataaaagct taattactat attttattgt gtgtttgtgt gttttttgtg
     2581 gatttttatg tcaataaatg atatagtata gataattaaa tgtattaaat atatttaatt
     2641 tttttatttt ttgagtttta taagtttatt tttatatatt acttatatat tatttatatt
     2701 ttgggttaga tacactttga catattttca aaagttaaaa gattaaaaat aaagtagaaa
     2761 tttatgttaa gtaaggagat taatatgaga agaaatacaa aattattaac aacagggatt
     2821 ctttcaatgg caatcgtcgc acctacaatg gcatttgcta ctgaatctaa tgctatggaa
     2881 aataacgctg atttaaatat aaacttagag aaaaaaagta tcgttttagg tagcaaatca
     2941 aaagttagtg tcaaatttaa agaaaaacca gatgcagata gcattacatt aaagtataaa
     3001 tgctatgaca tgccattgaa tacaactcta aattacaatc aatcaactgg ggcatatgaa
     3061 ggaactatca attataacca agacccagaa tatctaaatg tttgggaact acaagggata
     3121 acaataaaca gcaaaaataa tcataaaact ttaaacagac aagacctaga aaagctggga
     3181 ttaaatttaa aagactataa tgtaacacag gaatgtataa ttgaagatat aacttctaga
     3241 aaagatgtaa ataaatattt gagaaaaact tcttcaccta ttacagaact tacaggaagt
     3301 gatagatatg aaacagcagt taaaataagt aaagagggct ggaaaaatgg ttcagataag
     3361 gtagttataa taaatgggga tgtaagtata gatggcatta tatcaactcc actggcaacc
     3421 acatataatg caccaatact tttggttgaa aaaaacaatg tacctaatag tgtaaaatca
     3481 gaattaaagc gcctaaaccc taaagatata attataattg gagatgagaa tgctatttct
     3541 aaaactactg ctaatcaaat taaatcaact gtaaatgcta gtcaaacacg tttaaatggt
     3601 tctaatagat atgagacatc tttattgata gcaaaggaaa tagataaaaa tcatgatgtg
     3661 gaaaaagtat acataacaaa tgctaatggc ggagaagtgg atgcacttac tatagcagca
     3721 aaagcaggtc aagacaagca accaattata ttaactgata aagatagtat tacagacaat
     3781 acatataaat ggttaaagag tgaggattta caaaatgctt attttatagg tggtcctcaa
     3841 atgatatcaa caaatgttat aaataaggta aatggaataa ctaaagatag tgttactaat
     3901 aatagagtat acggagcaga tagacacgaa acaaatgcaa acgtaataaa aaaattctat
     3961 acagatgatg agttagaggc tgttttagta gctaaatcag atgtacttgt tgatgcttta
     4021 gcagcaggtc cattggctgc gaacttaaaa tctccaatac ttataacacc aaagacgtat
     4081 gtatctgcat accataaaga taatttagaa gctaaatcag ctaataaggt atacaaaata
     4141 ggaggaggat tgacttctaa ggtaatgagc tctatagcat catcattatc taaacacaat
     4201 acgactccaa cagaaccagg aaatagtggg ggcaagacag ttatgattga cccagggcat
     4261 ggtggttcag cacctggaaa ttcatctgga ggaatgattg aaaaagatta caatttaaat
     4321 acttcacttg caacaactga atatttacgt tcaaagggat tcaatgtaat aatgacaaga
     4381 gacacagata agactttatc tcttggaaat agaactgctc tatctaattc attgaaacca
     4441 gatttattta caagtataca ttataatggc tcaactaata aacaaggtca tggtgtagaa
     4501 gtattttata agcttaaaga taaaaatgga gggactacta aaactgtagc taccaatata
     4561 ttaaatagaa ttttagagaa atttaaactt acaaatagag gtataaaaac aagagtactt
     4621 cctagtgatt ctacaaaaga ttatttatac gttttaagaa gtaatgatat gccagctgta
     4681 cttgtagaat gtgcattttt ggataatgaa aatgatatga gtttaataaa ctcatctgca
     4741 aaagtaaaag aaatgggtac acaaataggt aaaggaatag aagattcatt aaaataaatg
     4801 gaaataaaaa tatttaaaaa cttaatgtaa atttatagtt gtaaatttat actaaagtca
     4861 aaaaatgaaa tataagtgta tcaatagtaa gactgtatgt acatttgcac atgtgaatgc
     4921 aagtatatat acagtctttt tttgtagtta aatgcaataa tttgattgtg ttttgtaaaa
     4981 tacacaatat gatataatta gataatatac acttaattta attgaggaag gtgattatat
     5041 ggaatattta tttgaaaaaa gtagtttaaa agaatatgga aatgaagctt tagataaata
     5101 tagagaatta ttagataaaa ctggtaataa ttcaaggtgt attcttttgt tagtaccaaa
     5161 caataacaca aagatacgat atgaaagggc tctaagatta aattatagtg aagaactgaa
     5221 aataacaaca tatataagtt ttgtgaagaa agaattagtt aaatactggc cactaataat
     5281 agaaaaatgt gatgaaataa gtacaaaggt tgtatctcca acatttatat caaatagttt
     5341 aagtgaatat ttaatagtgc aaaaagttaa ggaaaaaagg atacaagaag gatattttgg
     5401 agatattaca ggaaccaata aatccatagc aaatagtata atgactaaca taaataagtc
     5461 tgcatttaat ttaattgatt ttgattttat tggagaaaaa atatattcat caaagaaaaa
     5521 taaagatagt atatatagat tttcatacac tcaaatggat gaaattatat catattatat
     5581 aaattcctta ctatcaaatt caattttaga taatgcatta agcatatatt tatacaatca
     5641 atacctactt aatgatgatt tttatttaaa aaacttattt acagaagtga gatatataat
     5701 tgtggatagc cttgaaagct caagtaatgc agaagttgat tttataacag aagcattgga
     5761 taatacatta caaagttata tatattttga ttatagtagg gattattctg tatttaataa
     5821 tgtggatata gactatataa atgagaaaat aatatcaaaa attaagccta aagaagaaaa
     5881 tattaaaaag aaggagatta gaatagaaga tttatactta ttacctgcaa atatagaatt
     5941 aaatcaatca agtcaattat ataatgaaat gttagattta ataagtgaaa aagttatatc
     6001 tcttatagaa gaagatgtat ctccaagaga tatagcaatt atatctccta taaacaatag
     6061 tatattggag catcaaatta gagattcttt gatagaaaaa aatatagatg tttttagtac
     6121 taagaaggat aaaaaagctg tggattatcc atatgggaat gcattggtag tggctacatg
     6181 tatattctat ggatatttag attttataaa agatgatgaa tacttaagct ttttagaaac
     6241 tcttttagaa ataaatagga taaaagcatt taagattttt aatgaaacaa gacatttaag
     6301 tattgataat atagatgaac atagtcagta tcgtgatatt ttacagtata ttgaagaaaa
     6361 aaagaagagt gatataaaaa tacatgaatt tttaactcaa ttttacatag ataaaatgtt
     6421 aaatttaaaa gaaggtaaaa agaatgttgg gttatgtaaa aagataatag ctgaaagtga
     6481 gtcttttagt gaaagtttaa gtctacttgg gatgaaaaaa gaaaagatat ttatagaagc
     6541 tttaaaaagt acaataaacg attattactc tgttgtcgat atagaagagt tgaaatccaa
     6601 agacaaaata gtaataacta ctccttattc atatatatca tcaaatatag atagaagtat
     6661 acaaatatgg gtagatatag gaagtaatgc ttggaatatg aaaatagaaa aagatataag
     6721 taatttaatt gtacttagaa aatcttttga agaaaaaaag atttatacaa atgaaatgga
     6781 agaatattat aaaaaatatt atctatacaa tacagtatat aatttgttat taaatgcaaa
     6841 gaaaatctat gcatttaaaa gtgaatatgc gattaatggt tatatccaag agagtatatt
     6901 gtatagtata cttttaaaaa tctcacataa aggagataaa aattatgatt aatataaatt
     6961 atagagaaga ccagatacca ataataaatt acgataatgg tactatggca gtaccagcag
     7021 taccaggtgc tggaaaaaca tttataataa caaatttagt agctaaacta ttacttgaac
     7081 agaaacataa gggtggtaaa atattaattc ttacatatat gaatagtgct gtaaataatt
     7141 ttaaaggtag aataagaaag attcttgaag agaataaaat agatgatact aattcgtatg
     7201 aagtaatgac tatacacagt ttggctgtaa aaataataaa ggaaaagcca gaagttgtaa
     7261 tgttaagtga ggattttaat atagctgatg atttgcaaaa aagtattata ttaaatgaat
     7321 gtataagtag atttagattt gaaggtggag aaagagcttt tagatggttt ttaaaggagc
     7381 aaaaagatga acgctggaaa gaaataactc ttgaagcctg ggaaagaggt ttttttgaat
     7441 ttgcaggaaa tgcaataagt gaacttaagt ataagggaat ttctccagat aaacttgaag
     7501 aaatattgtc aagaggacac aaaggaatgc ttaaaattat tttaccaata tataaattgt
     7561 atgatagaaa attaaagcaa aatggattgc tagattatga tgatatatta attttagcag
     7621 aaaaaacttt aagcttagac gaagggctta gaaagaagtt tcaaactaga tacaaatata
     7681 tatttgaaga tgagtgtcag gactcaaatg aaattcaagg taatataata aaaattatat
     7741 ctagcgaaaa taaaaaccta gttagagttg gagatataaa tcagagtata acaggaacat
     7801 tttcatcatc agaccctaaa ttttttaaag aatttataaa tagtgcagat ttctgttata
     7861 ggatggatat gtcaaatcga agttcaaaag atatacttga tttagcaaac actcttgtca
     7921 agtatgttac aaaggaattt agacagaaag aatgtagaca tgctcttgaa gatatgcaaa
     7981 taaaaactgt gccaaatgga atgggatata aagaaaatcc taatccagaa cattataata
     8041 taaatgtaaa gtggtatgag agttggaaaa ttgaaataga gcaaactgct agatacgtta
     8101 agggtataaa aaataaatat ccagataaaa gtataggtat attggttcca tttaatgaac
     8161 aagtgacaca agttgctaaa gaacttagag agaataatct aacctttgaa gaattaggac
     8221 caaattcttt gaataaaagg aaggttataa acaatatagc atatattatt gattttatgt
     8281 tacattgtga tgatgtagaa aagttaatag ttgtacttaa taaagttttt atagatactg
     8341 ataatgaaga agaaataaag tattttttag aagaattaaa aaactattct accgaagaaa
     8401 ttatttataa ttttgaattt agtaagttag ataatgattt aatagaattt aaagaagaaa
     8461 gtgatatata cttaagtttt aaaagaggaa tagatattat aaaacagata ttagaatatc
     8521 caataataag gttagacctc ttagttttgt ttattgggaa aaaacttaat ttagaaaaag
     8581 aagataaagc tgtactggat tatatatctt tttatataaa gtatctagta tcagaaaata
     8641 taaatatgga tttagaagat gtatataatt tattgtttga tgtaaagaac agggtgttta
     8701 atcatataat agatgtggta tatgagataa atggatatga accagagccc ggtagtataa
     8761 cactatgtaa ctatcataaa tcaaagggga tggaatggga ttgtgtgttt ttactaggtc
     8821 ttgttgaata taatttccct gacaatatta atcaaaagtt tcagagtgat aaatggtatc
     8881 taaaagaaaa atataaaaac cctatggcaa ttattaaatc agaagttgaa gctatattaa
     8941 aaggaagtat ttcaacagat tatgctcata aaacgaagat tgattctata aatgaaaaga
     9001 ttagactttt atatgtaggg attactagag caaaggaaat gttagtactt tcaggaagtg
     9061 cttatagaga tgaaagtgat atagggaata agagaaaaga gcaaaaacca tgtatatatt
     9121 taagcagact taatcaacat attattgaaa aaaggtcaaa ttaatatata ataagttatt
     9181 aaaagattgc ttgaaatata tctaaaaggg gataaaacaa tatgtacagt aagtaactac
     9241 taaaaaataa agttaagaga gaagtgatat gagtgaacga taaattaaaa tatttttctt
     9301 atagtcaaaa ttcaattaac acatataaat catgtccatt gaaatttaaa tataaatata
     9361 ttgatagaat aaattggaaa aatgatgatg ttggtagtag agagtattat gaaactttaa
     9421 aaacaggaag agattttcat ttgatttgtg aaagatattt tagcaatata cctcttggaa
     9481 tctattttaa tgaggatgat aaaaatagta aaaagttttt aaagtggatg gaaaatataa
     9541 aaaaagttgt tcctattgaa aaaggaaaaa cgtatttgcc agaatatgaa gtaaggatga
     9601 cattaaatgg ggatataata caggcaaagt atgatttagt tgtaattgga aataacaata
     9661 tagaaatatg ggattggaaa accgagtcta aaaagattga ctataaacac gtagaaaata
     9721 gaatacaaac tattgtttat atgtttttag caaaagaagt aatacctaaa gttttaaaaa
     9781 tggacataaa tgttaaagat ataaatatga gatattatca acctgaattt gatgacttac
     9841 caattaccat tttatatgat gaagaaaaac atgaagcaaa tagaaataaa attcaaaatt
     9901 atataagtat gataaaaaac actaattatg aagaacatac atacgaagac aatctataca
     9961 atgatataga aaaggtatat aataaagaag aacgtatgtg ctatagaaat aaaaagcatt
    10021 gcaagtattg tgaatttaat aagttatgta atggcaaaga aatagattat agtattttgg
    10081 aggcggaaat ttatgggact taggtttgta ttaggtagaa gtggaagtgg aaaatccacc
    10141 tatatactag atgagattaa aaaagaagct caaaaaaatg aaacgacatc aattatactt
    10201 ttagttccag aacagtacac atttgaggct gaaaatagag taagtaagct attcttagga
    10261 aaagaaaaag ataagtatct aagagttagg gtacttagct ttaagacact aagtaatata
    10321 gttttttctc aagtaggagg attgacagat gtaaatataa attctagtgg aaaagcaatg
    10381 atggtatata gagctattga agatgtaagt gaagaattaa atgtgttttc aaaatcaaaa
    10441 tcgcagtctg gttttgtaag ttcaattaca gatatgatat cagaaatgaa acaatacaat
    10501 ataagccctg aaatgttaga aaatatatct ggagaattgg ataatgaaac tttaagttta
    10561 aaattaaaag atataagtaa aatatataat tcttttgaag ggaaattgca tgaaaattat
    10621 gtagatgctc aagatatgtt aacttcattg gcaagtaaaa ttgaattaag cagttattta
    10681 gatggagctt gtgtttatat agatgaattt acaggtttta caccaaatca gtacaatgtc
    10741 ataaagagta tattaaataa gtctaaaagt gttaatatct ctttgactgt agatgatatc
    10801 aactatattg ggtacagtaa atctgatatg ttttctagaa cgaaatttac ttattcaaaa
    10861 ttgactcaat tgtgcaacga agaaggcata aaaatattgc cacaagtaaa tttaaacaca
    10921 ggtgttataa aaagatttga aaaagttaag gaattacagc acttagaaag attttataat
    10981 gcttatccat ataaaatata ttcaaatcca actgaaaata taaagataaa agagtttaac
    11041 aatttatatt cagaggtgga agagatagcc agagaaattg tacatttagt tagagataag
    11101 aatgtcagat atagagatat aaccattgct acaagagatt taaatagata tgatttttta
    11161 gtacactcta tatttaatga atataatata ccaaacttta tagataagaa gagagaggca
    11221 aaaagtaatc caatagttat tttaattatt tcaactttag aaatgaagaa tagacgatat
    11281 gggtatgaaa ctatgtttag gtatttaaaa agtggactta taggaattga taatgatgat
    11341 ataaatttac tagaaaatta tgtgttggct aatgggatta aaggtaaaaa atggtttgat
    11401 gaaaaatggg attatagaat aactcaaagt ctttcaggtc aagaaagtga atttgaactg
    11461 gaattaaaag agaaaataaa tgaaataaaa aatagagtat tagagcctat agtaatttta
    11521 caagaaaaat taagaggaaa aaatagagta aaagaaatct gtagatatat atatgaattt
    11581 ttattagata taaatatgcc agaaactata gaaagtttaa tagtaaattt taaggataaa
    11641 ggggaacttg atgttgcaaa tcaatactca caagtttggg acatagtagt ggacatatta
    11701 gaccaaatgg tggaacttat gggtgatgaa ataatttctt tagaaaaatt tataaaatta
    11761 ataaccttag gttttgatga atatgaatta ggactggttc ctcctagtat agaccaggta
    11821 cttgtaagta gtgtggatag aatgaaaaat ccagacacga aatatttata cctagttgga
    11881 actactgatg gtgtgtttcc gctgattaca aaggatagtg gaatattgag tgataatgat
    11941 agggaaagtc ttggaaacaa aggaatcgaa gttgatattg atagtaagac taggagtttt
    12001 gaagaacagt ttcttgtata taaagctctt acttcgacat ctaagaattt aaccataaca
    12061 tatccaatat cagaccatga aggtaaaacg cttagaccat caataattat atcaagactt
    12121 aagaagatat ttccaaatat agaaaataaa agttatttag tggaagaaaa taaaagcact
    12181 gataaagata tacttaaaaa aataacggtt aaatccccta catttaatga acttataaac
    12241 ataataaaaa attatgacag tgatggttac aatacagaag agattaatag tatttggtta
    12301 gatatatata ggtactattt aaaagatgaa gtctatagtt ctataacaaa aaaagttata
    12361 aaaggtctta gttatactaa tcaagttcat aagattgaag agaaaaaaat aaggtcattg
    12421 tataaaagta attctcttag catatctagg cttgaaaaat atgctgaatg tccatttgct
    12481 tattttatac aatatggtct aaaagcaaaa aagcgtaaag agtattcatt tacaccacca
    12541 gatttaggaa cttttattca taatatttta gataggtttt caaaagaact tttacaagat
    12601 aatttaactt ggagagatat tgatgaaaaa tatatagaat taaagatagg tataatagtt
    12661 gatgaaataa ttttaaagat accaggatat atattaaata gttctgagag gtataaatac
    12721 cttgcatata gattaaagaa tatgttgaca actgctataa ctataattag tcagcaaata
    12781 aaacaaggtt catttgaacc aatagattat gaagttaaat ttggcaacaa tggaaaatat
    12841 ccacctataa aaatggtgtt agaaaatgga caagaagtaa gtttaattgg acaaatagat
    12901 agagttgatg aatttgaaga aggagaaaat aaatatataa gaataataga ttataagtca
    12961 ggcaataagt ccataagtct aacagaaata tattatggtc tacaattaca attacttgtg
    13021 tatttagatg caatccttga aagtgcaaag gatgaagata taaatataaa tccagctgca
    13081 atactatatt gtagaataaa taatccaata gcaaaattta atgaagataa agatgatgaa
    13141 gaaatacaag aagcaatatt aaaagaactt agaatgaagg ggcttgtagt aaaagactct
    13201 cacatagtta aagagatgga taaatcatta atagatggag aaaggaaaaa ctctttagta
    13261 ataccagtag gtcttactaa ggatggaaat gtaggtaaaa gtacttcagc aataagctat
    13321 gaggatttca aactgcttag aaaatatgta aggcatgcta taaaggattt atgtgaagaa
    13381 atgttaagtg gagaaatacg tatagctcca tataaacata aagatggaac atcatgtgac
    13441 ttttgtgatt actctgctat atgtcagttt gattcaacta taaaagataa caagtataaa
    13501 aatttaaata ataagagtaa tgaagagata ataaaaatga tgaaaggaga tgttaattag
    13561 tgagttctcc taaatggaca aaagagcaat tagaagttat agaaagtaga gaatgcaatt
    13621 tattggttgc tgctgctgct ggttcaggaa aaacagccgt gctagttgag cgtataattc
    13681 aaatgataac aagtagagaa aatccaattg atatagataa gttattagtt gttacattta
    13741 ctaatgctgc tgcgtcagaa atgagagaga ggatagggga tgctatagga aaagccttag
    13801 atgaaaaccc agaaaataaa catttacaaa accaacttgt tttattaaat aaatctagta
    13861 ttacaacaat acactcattc tgtttggatg ttataaaatc aaactttcat agaataaact
    13921 tagaccctaa tttcagaata ggagaccaaa ctgaatgtgc tattttgaaa caagaagcaa
    13981 tagaggaagt atttgaagat ttatatgaag aaagagatga ggggttttta aatttagttg
    14041 aaagttatgc agaaagagga ggagataaag aagtacaaga tattatcttg ggaatatatt
    14101 catttgcaat ggcttctcct gaacctaaaa aatggttgat tgactcagca gaacgattta
    14161 atattgatga aaattttgat ttttcacagt ctatttgggc tagagctata ttagacactg
    14221 taaaaataga gattaatggt ctttgtctaa atatggaaag ggcattaaaa gaagtggaat
    14281 caatagaaga attagaaact tttgcagaaa agttatctgt tgaatataaa aaaattgctg
    14341 acatttcaca agcatgtaat aaatcttggg atgaggcata taaaaagatg gcaagtatgt
    14401 cctttgaaaa ttatgtaaaa ggagtaaaaa gaatttcaaa agatgctcct tcgtatataa
    14461 aagaatcaaa agaaaaggca aaaactataa gagataagac taagaagtct ttagaaagta
    14521 tagtaagtgc tacattcaat aaagataatg attctattag agaagaaata aaatatcttt
    14581 ataacatagt taaaccaata tctagtgttg tactgagatt tgaagaggaa tatagcaata
    14641 agaaaagaga aaaaggaata atagatttta acgatataga gcattttgca ttaaatatac
    14701 ttacagatgt agatgaaaaa ggtaatattg taccttctga tatagcagtt ggatatagaa
    14761 acaagtttta tgaaatattt atagatgaat atcaggatag taatctagtg caggaagtac
    14821 tgcttaaagc tgttgcaaat acagaaactc caaatagatt tatggttggt gatgtaaaac
    14881 agagtatata tagatttaga caagcaaagc cagaattatt tttgcaaaaa tataataact
    14941 ataatgataa aaagggaagt tcccatagaa aaataatgct ttacaaaaat tttagaagta
    15001 gagaagaagt tgtagatgct gtaaattata tatttgaaaa tataatgaat gagaatatag
    15061 gagaaattga atatacagaa aaagaaagac taaatttagg tgcaaatttt aatgtggata
    15121 cagacgagaa atctattatt ggtggagcaa cggagataca cctaatacaa aaagataata
    15181 aacttgatga tgatattata aatgataaag acgatagaat taataacaaa gaagatgaaa
    15241 tagaggaaga agaaaaatta gataatatac aacttgaggc tagaatggtt ggaaacatca
    15301 tcaaagattt aatgaaagtc aatgaagatg gaaaaattca aaaggtctat gataagggaa
    15361 tagatggata tagacctgtg gaatttagag atatagttat tcttttaaga gctacatctg
    15421 catgggctcc agtgtttgca gatgaattga tgaatatgga tataccaaca tatgctgatg
    15481 ttggagtggg gtattttgat acgattgaaa taaaaacaat attgtcatta cttcaaataa
    15541 tagataatcc aatgcaagat atacctctta tatctgtatt aaaatcacct atatttggat
    15601 ttacaccaga ggatttgatt gatattagag ttcagagtaa agataaaatt ttttatgagg
    15661 ttttaaaaag tacagcagag tatgatggat ttacagattc tcaaaatgaa aatgagtctg
    15721 agtttatacc aagtgaagag tgcataaaca aaagtaaaga ttttttaatt aagctaaagg
    15781 aatttaaaga aaagtcaatg tacatgagta cagatgaatt tatatggtac ttgtacacaa
    15841 ggactggata ctatgcctat gttggagcat tgccaggtgg aagtcaaaga caagctaatt
    15901 taaaagtatt gtttgagaga gcaaaacaat ttgaagaaac cagtcttaaa ggaatattca
    15961 actttgtaaa ttttatagag aaattaaaaa aatctagttc tgatatggga agtgcaaaaa
    16021 cacttggtga gaatgcaaat gttgttagaa taatgagtat tcataaaagt aaaggtctag
    16081 aatttccagt agtcatatgc tctgcaatgg gtaaaaattt caatactcaa gattttaaaa
    16141 agagtatttt atatcatcat aatttagggt atggacctca atttgtagac tatgagagaa
    16201 ggatttcttt tccaagtata gccaaagaag cattaaaaag caaaataaat atagaaaatt
    16261 tatcagaaga gatgagagtt ttatatgttg catttacaag agctaaagaa aaattgatta
    16321 taactggttc tactagaaat atacaagata gtataaagag atggtcaaat ggaattgaaa
    16381 gtttagatac aatatcacaa tacgagatat taaaaggtaa aaatttttta gattggataa
    16441 tgccttgtgt attaagacat agggatttat ctaatctact agaagaagta ggattagacg
    16501 cagtttttaa tgttgaacat aattcaaaat ggtatggaaa gttgtggaat aagaatgata
    16561 ttttagtgga aaagaagagt gatgaagaaa aagaaagtat tgaagaaatt cttgaaaaaa
    16621 tagatgtaga taatcctgac agtgattatt acagtgaaat agaagaaaaa ttaaactata
    16681 tatatccata tgaatttagt acaagaaaac ctgctactat atctgttaca gaaataaaaa
    16741 agatacaaaa taattatgaa gaagagttaa taaatactat atttgaacaa aaagtaatat
    16801 taaaaaagcc tctatttatt caaaatgaag aagaaagaga aaaaataagt ggaacagaaa
    16861 gaggtacaat agtccattta gttatggagg ttttagactt aaaaaatgta agtagtgtaa
    16921 atgatataaa atcacaaata agaggttttg tttcaaaagg tataataaca gaaaaacaag
    16981 ctagtatagt aaatccatat aaaatatata aattttttgc ctctaacata ggaaaaagaa
    17041 tgttgaatgc tgaaattata aatagagaaa aatcaattta tgcacaagta aatatgaaag
    17101 acatatatat ttatgaaaag cttataaaca atgacgataa aaaactttat gataatgaaa
    17161 gtgtcatgtt aagaggtata gttgatgctt attttgaaga agataatcaa atagttttgg
    17221 tggattataa aacagatttt gtaaatgagg aaaatataaa tcaaataata gagaaatata
    17281 aaaaacagtt ggatttatat gcagatatta ttgaaacttt aactggtaag tcagtaaaag
    17341 agaaatgtat atatttattt ggagttgatg aagctgtttg ttattagttt ttatatataa
    17401 attaaaccta aaagatagag gaaggggaaa aaatgagatt tattcataca tcagattggc
    17461 atctagggaa gtccctagaa ggccattcta gaattgagga acaagcaaag ttttgtgagg
    17521 aatttataaa aatagtagaa tcaaatgaaa tagatatggt tataattgct ggagatgtat
    17581 atgatacttc taatccacca gcacaagcag aaaaactatt ttatcagact gtatcaagac
    17641 ttgccaataa tggtcagagg tgtgtattaa taatttcagg aaatcatgat aatccagaga
    17701 gactctctgc aataacacct ctagcacatg aacaggggat acttatatat ggttatccat
    17761 taagtgctac gatagaagct aagtataaag ggtttgaaat aacatatgca actcagggat
    17821 gtacaaaact gaatataaat ggagaaaata ttgtggttgc tacattacct tatccaagtg
    17881 aaaaaagatt aaatgaagtt ttttcaagtg aagatgagtt tgagaaacaa aaaaattatt
    17941 ctgaaaaggt aggagatata tttagaagtt tagaagaaaa ttttagaagt gatactataa
    18001 atatagccgt atcacatata tttgtaatag gtggagagag tactgaatca gaaagaccta
    18061 tacaattagg tggaagtttt ttagttgaaa gaaaagattt gccagaaaag gctcaatata
    18121 ctgcactagg tcatttacat aaacaacaaa aagcttccga acgtttaaat gcgtactatt
    18181 ctggttctcc acttcaatat agcaaggatg aaagagcgta taccaaaggg gcatacatag
    18241 tggatataaa agctggtgaa aaaccaataa ttgaagatgt atattttaat aattacaaac
    18301 ctatagaagt atttaaatgt aatggaatag aagaagcact agatatatgt gagcaaaatc
    18361 aagatagaga gatttggtca tattttgaaa taaatactga tgagataata tcacagaatg
    18421 aaattaaaaa aatgaaggaa ctattaaaag acataataga aataaaacca ataataacat
    18481 cttgttatga acaagaaagt gtagatataa aagaaaagtc tatggcagag ttgtttagag
    18541 agttctattc ttttagtaaa ggtgtagagc caaaaggaga acttatggat ttatttctag
    18601 atataataag tgaagaaggt gaatcagcag atgagaccaa ttagattaga acttacaggg
    18661 cttaatagct atattgataa acaagttata gactttgaaa aattaattga aagaggtttg
    18721 tttgggatat ttggaactac tggaagtgga aaatctacaa tattagatgc aataactatt
    18781 gctatgtatg gaaatatatc cagaaataca aaagaatata taaacagtgt gtgcgataag
    18841 gcaattatat catatgagtt tgagatagga agcaaaaaca ctagaagaag atacattgta
    18901 gatagaacca ttgctagaag caaaacagga acaaagacct ctaatgctag attaatagaa
    18961 gtattgaatg acaataccca aaatgtttta gctgataaag ttgtggaagt aaatgaaaaa
    19021 gtagcacagg ttgtaggatt aacggcaaat gattttacta ggtcagtagt tttaccacaa
    19081 gacaaattta atgagtttct aagattatca cgtgcagata gaagagacat gttagagaga
    19141 atttttaatc ttgagaaata tggaagaagt cttggagaaa aagtgaaaaa gagaaaaaat
    19201 atacagttac aaaatttaaa agatttgaaa tcaaaattaa gtcaatatga tggaattaca
    19261 gaggaagtat ataacaatat aaaccaagaa ttaatagagt taaaaaattt ggaaaaagat
    19321 aagaataatg ctttagacct agctcaaaaa tcttatgaag aaagtaaaac agtatatgaa
    19381 gaacaattaa agttagaaaa aaatgaactt agaaaaaatg agcttgattt aaaaaatagt
    19441 gagataaaag aaaagattgc tcttgtagaa aatgatagta acgctaaaaa ggtagaccca
    19501 tacataagtt cagttcaaaa tctagaaaaa aagatagatg aagatagctt cgcagtaagt
    19561 aatttagaaa agaaattagc aatattaaat caggagcttg aagttacaaa aaatagatat
    19621 gaaaaaataa acaaaattaa gaatgaagaa gtccctaaat tgagtgaaga aaaaataaga
    19681 ttgcaacaag caataaaatt agaagaagag ttagttttat tagatagaga gttaaaagag
    19741 ctaaaagaaa gtggaattaa tttaaataag actaaagttg aattagaaaa agtaaagcag
    19801 gtgtcagagt ccagaaaaga tgcagttact aaatctataa aagaagttga aggaaagatt
    19861 gataaagtaa atataagtgc agaattaaaa caaaaaatat ttctagcata tgaatacgag
    19921 aaagattaca acaacgtatt ggaagaaaaa aatcaaaagc taaataaact agaagaaata
    19981 ctcaaagaga cagaaaatat aaatttaaaa gttagatata ttgataaaga taagaatgat
    20041 gtaaatcgta atttagaaaa tttatcactt cacttggatg ttcttcttaa aaaatgtcct
    20101 ggtaagagtg ctgatttact tttaaaatct gaatatgtta ctgaattaag aaataaagca
    20161 aataatacta aagaaaatga aattaagaaa agcagtatac aagatgaatt aaagataata
    20221 cttgaaagta agtttaatac tgaaagggaa gtaaatttac taaatgaaaa acttgaaaat
    20281 aatagaaaga acagagatga cctagaaaaa gaattagaag aactaaagta tttaaattta
    20341 gcctctgaac ttagacgtga attaaaagaa aatatgcctt gtccagtttg tgggtcaaaa
    20401 catcatgaaa atcataatat aactaaatat gatgaaaata tatcttttgt caaagaaaaa
    20461 ctggagaaat tagaaaaaga aaaaataagt ataagaaaca atatagagga attaaatgct
    20521 aaagtaagtg ggtatttatc tatagaaaaa atgaaggcta aagaacttga agatgtaaaa
    20581 ggaaaactag gggggattcc atctagtcaa ttattaaaaa aacttgatga agaacaaaga
    20641 aaacttgcat tgttaaaatc taatattcag gagtgggaga aagaaaaaga gagtactgaa
    20701 aataaagtaa cattagcgaa agaagaaaaa aataaaattg aaaaagaaga attaaaaata
    20761 agagagagtc tgaataatta taagaaatta acaagagatt taaacataga aatagaaggt
    20821 ttagaaaata aatgtaaaaa acttaaacag gaatacctag gattaaagac gattactaaa
    20881 gtctctaatc tattgtcaaa agtaaaagaa ataagggaga atgaaaaaga attagaatta
    20941 ctaagtactg ggcattcaaa tttattaaaa aatagagact cactagatat taaaataaga
    21001 gaaaatgaga gtaaactaca tgaaatagag atagaattaa taaaggctag agaattatat
    21061 gtagaaaaga aattgagtag ggataataag tataaagaag ttattagcat aactaaaggt
    21121 gatttagcta aaaatctttt acataatgtt gaagaaaata tatgtaagat attagaacaa
    21181 gaagaatcaa gcaagaaaaa attggaagag caaagattag aatatgaaaa aaacattgct
    21241 gaaaagcaca atatagatgg tagacttaaa actgctaagg aacaatataa agaccaaaaa
    21301 tcaacactaa ataaactatt agcagaaaat aaatttgaaa gtatatatgc agtaaaaaga
    21361 gctttattag atactgatgc agtaaaaaaa ttgattgaag aaatactaga gcatgaagaa
    21421 gaacaaaagt tattatcatt taaaataaaa tcatcaaaag aaaaattaaa tggaagaagc
    21481 ataaagaagg agtactttga acaattaaaa gatgaaattt ataatttgaa agtagaaata
    21541 ggaaatatat ccaaagatat tggagctaat caaaatcagt taataacatt aaaagactct
    21601 ttagataaga taaatgattt taataaacaa cttaaggtag ttgaacacaa tgtggactta
    21661 ttggaagaat tagataaatg tgtacaagga aataaatttg tagaatatgt ttctacaaac
    21721 caattaaaat atatagcgtt ggaagcatca aaacgattgg atggaataac aaaaggaaga
    21781 tatgcacttg aaattgactc aacactaaac tttgtaatga gagataactt taatggagga
    21841 gaaagaagaa gcattgatac tctatctggt ggagagacat tcttgacatc tctatcatta
    21901 gcacttgctc tttcatctca aatacaatta aagggaagtg caccattaga atttttcttt
    21961 ttagatgaag gttttggttc attagatagt gaacttttag aaattgttat agaatccttg
    22021 gaaagattgc atagtaataa tctaagtgtg ggtataataa gtcatgtcga ggaacttaaa
    22081 aatagagttc ctgttaagct attagtttca tctagtgagg ctggaatagg ttcaaaagta
    22141 aaaattgaat atagttagaa aatatatgtt aattgaatat attatctaat atttccaaat
    22201 aatatgtaat tgtgatataa taaaaactaa tattaaagtt atttataatt aatattaata
    22261 taaaaacatg taagtaggag tgtggtaagt gttggaatcg cccaataatt tgattcagat
    22321 tattttttta atagtattac ttatagggtc tgcatttttc tcagcatctg aaacggcctt
    22381 aatgtcgttg agtaaaatca gaatcagata tatgcaagat gaaggagtta aaggagctaa
    22441 gttagtaagt tcattaatag aaaatccaaa taagctttta agttctatat tggttggaaa
    22501 taacgtcgta aatatagcag caacatccat atcaacatct ttgtttatag gattaatggg
    22561 agaaaaagga gtggcacttg ctacagctgt tatgacagta ttagtattaa tatttggtga
    22621 gattactcca aaaacaatag cagcaaataa ttcagaaaag gtttctctat tagtatctaa
    22681 acctataaaa gcaattatat ttatattaag accaatagta tggatattta atataattac
    22741 aaatattata tttaaattat ttggtataac aaataaagga gctaaatcat tcataactga
    22801 agaagagcta aaaactatgg taaatgtaag tcatgaagaa ggcgtccttg aaatggaaga
    22861 gagagaaatc ataaataatg tttttgaatt tggagatatg caagctaaaa atgcaatggt
    22921 acaaagaata gatatggtag ctatagatat ggaagatagt tatgatgaga taatacaggt
    22981 atttaaaact gaaaaattaa gcagaatgcc tgtgtatgaa gaaactatag atgatattgt
    23041 aggaatactt aatataaaag atattatatt tttatctgat gaagaaatag agtcatttga
    23101 tattaaaaat tatatgagag aacctttttt cacgtatgag ttcaaaaaga taacacaact
    23161 tcttgaagag atgaaacttg aaaaaagtca gatggctata gttgtagatg agtatggtgg
    23221 aacatctgga cttttaacta tagaagattt agtagaagtc attgttggag atatagagga
    23281 tgaatatgat gaagaagagg atgaaataca agttataaaa gaagatgagt atattgtaga
    23341 tggaagtact aaaattggtg atgttaatga acttattggt gtaaacttgg aatctgagga
    23401 gtttgattcc ataggaggtt ttataattgg acacctaagc agattgccag aagaaaatga
    23461 agtaattgaa gttgataata taagattttg tatagaaagt atagaaaaaa atagaatt
//
LOCUS       DBGJFF010000058        23119 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.059, whole genome shotgun sequence.
ACCESSION   DBGJFF010000058 DBGJFF010000000
VERSION     DBGJFF010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23119
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.059"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            101..1387
                     /locus_tag="ACOIAF_002206"
     CDS             101..1387
                     /locus_tag="ACOIAF_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006439082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HGZ2669215.1"
                     /translation="MRLELGKIFIKDIQFGDVTEVKDGVLFINKQEMLQEIGGDEHIK
                     SIDIELARPGESVRITPVKDVIEPRVKVEGNGGIFPGIMSKVDTVGEGKTHALKGVAV
                     VTTGKIVGFQEGIIDMTGEGAKYTPFSKLNNVVVIAEPIDGLKQYAHEKAVRMIGFKA
                     AMYLGEVARNLTPDEVSVYETKPLLESIKEYPELPKVGYVYMLQTQGLLHDTYVYGVD
                     AKQIVPTLLYPTELMDGAIVSGNCVSACDKNPSYVHINNGVVEDLYARHGKDINFVGV
                     IITNENVYLADKERSSNWTSKLCKYLGLDAVIVSQEGFGNPDTDLIMNCKKIEMQGVK
                     TVIVTDEYAGRDGGSQSLADADVRADAVVTGGNANQVVVLPKLDKVIGHLEVVDVIAG
                     GSDGSLRADGTIEVEIQAITGATNETGFGHLTAKGY"
     gene            1499..1972
                     /gene="grdA"
                     /locus_tag="ACOIAF_002207"
     CDS             1499..1972
                     /gene="grdA"
                     /locus_tag="ACOIAF_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_110943085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030700 - glycine reductase complex
                     [Evidence IEA];
                     GO_function: GO:0030699 - glycine reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:1631..1633,aa:Sec)
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     selenoprotein A"
                     /protein_id="HGZ2669216.1"
                     /translation="MSLLSNKKVLIIGDRDGIPGPAIEECVKTVEGAEVVFSSTECFV
                     UTAAGAMDLENQNRVKDAADKFGAENVVILLGAAEAEAAGLAAETVTAGDPTFAGPLA
                     GVALGLSVYHVVEEPIKSLFDESVYEDQISMMEMVLEVEEIEEEMSGIREEFCKF"
     gene            2004..3314
                     /gene="grdB"
                     /locus_tag="ACOIAF_002208"
     CDS             2004..3314
                     /gene="grdB"
                     /locus_tag="ACOIAF_002208"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030700 - glycine reductase complex
                     [Evidence IEA];
                     GO_function: GO:0030699 - glycine reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:3051..3053,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HGZ2669217.1"
                     /translation="MGKLKAVHYINQFFAGIGGEEKADTKPHVAETLPPISLQLDKLL
                     GEDIEIVGTVVCGDSYFNENIDSASEEVLSMVKGFEPQLFIAGPAFNAGRYGVAAGTI
                     TKVVKDALNIPALTGMYVENPGADMFKKDVYVVETSDSAAGMRKALPKIAKLAVKLAN
                     GEEIGTPKDEGYIARGIRVNYFHEDRGSKRAVDMLVKKIKGEPFETEYPMPNFDRVDP
                     SKAVKDLSKCKIALVTSGGIVPKGNPDRIESSSASKYGTYSIAGVMDLTEETYETAHG
                     GYDPVYANLDADRVLPVDVLRDLEKEGVIGKLHETFYTTVGNGTSVANSKKYASEIGA
                     ALVADGVDAVILTSTUGTCTRCGATMVKEIEKTGLPVVHMCTVVPISLTVGANRIVPT
                     IAIPHPLGNPALDPTEEKALRRGLVEKALNALTTEVDGQTVFEK"
     gene            3571..5103
                     /gene="grdC"
                     /locus_tag="ACOIAF_002209"
     CDS             3571..5103
                     /gene="grdC"
                     /locus_tag="ACOIAF_002209"
                     /EC_number="1.21.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     component C subunit beta"
                     /protein_id="HGZ2669218.1"
                     /translation="MTYPVLKGAGYVLIHTPDMIVQNGSTCTVERATNPDSEFLKEVS
                     NHIRSYEDVVNYMPNQVYIGNRRPEELRDLPMPWCEQKIEGTRNGKFGEIMPQDEFIA
                     LMQISDAFDLVKLSQEFIDEVKPKIENNYPEIAPFVGKLKGDDIEEGKELVATHIAEG
                     LYHDGKFVGYVKRAHDVDVNLNAHTMFENLVVKASGVLSAIQMLRHSKIDPAEIDYVI
                     ECSEEACGDINQRGGGNFAKSIAEIAGLQNATGSDTRGFCAAPTHALIQAAALVKAGI
                     HKNVMVVAGGASAKLGMNAKDHVKKGLPVLEDVVGGFAVLVSENDGVNPVIRTDLTGK
                     HTVGTGSSPQAVMTALITSGLDRANLKITDVDVYSVEMQNPDITKPAGAGDVPEANYK
                     MIGALAVKRGDLEKKELKDFVSNKGLPGWAPTQGHIPSGAPYIGFLIDDLTTGNRNRA
                     MIVGKGSLFLGRMTNLFDGVSFIAERNTGVTEETSGISKDEIKKIIAESMKKLALDML
                     EE"
     gene            5114..6259
                     /gene="grdD"
                     /locus_tag="ACOIAF_002210"
     CDS             5114..6259
                     /gene="grdD"
                     /locus_tag="ACOIAF_002210"
                     /EC_number="1.21.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     component C subunit alpha"
                     /protein_id="HGZ2669219.1"
                     /translation="MSKKVIADVFLEVANAIESGEFGKKVKIGVTTLGSEHGVENMVN
                     GAQLAKSNLFDIVLIGPKVETDLEVVEVNDEKEMHAKMEELLDSGYIDACVTMHYNFP
                     IGVSTVGRVITPAKGKEMILATTTGTSATNRIEAMVRNAIYGIATAKSMGNKCPKVGI
                     LNVDGARQVEKCLKELKDNGYDMEFADSIRADGGCVMRGNDLLVGAPDVMVTDTLSGN
                     IFMKVFSSYTTGGDYEAQGFGYGPGVGEDYDRKVLIVSRASGSPVVANALKYAYDVVK
                     GDISNVARNEFAKVKKAKFDDIISSLTKKEVKAEKVEVKMPDKEIVTRQIAGVDIMDL
                     EDAVSELWKNGIYAESGMGCTGPIVLVNEAKGDLAVKTLVKAGYTAK"
     gene            6569..7648
                     /locus_tag="ACOIAF_002211"
     CDS             6569..7648
                     /locus_tag="ACOIAF_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HGZ2669220.1"
                     /translation="MSRVKNVVELLETKGVDALYLTKKTNVNYISGFPDEEAYAVICK
                     DGNFLVTDSRYMELAEKVCKDFEIINWHNFDRSVAKAVKSVCDKVGIKKLGFERTNIV
                     FDKYEELKNLIEKDNGELIPTENIVETLRYVKDKDEIKNTRKACEIADKALEELIPHI
                     KAGVSEIELATRLEYFMKMNGAQNIGFETILISGAKTSLLHGKPSDKIIEKGDFVLID
                     YGAMYNGYISDTTRTFIVGGASEKQLEIYNLVKEAQNVGVENMKAGVHATIPDAEIRK
                     VVKKYEDYYYQGIGHGVGRDVHEEPFIGNYGDKIIEEGCIITMEPGIYFPGWGGVRIE
                     DTVLITKNGPERLTKFPKDLMILDK"
     gene            7761..8360
                     /locus_tag="ACOIAF_002212"
     CDS             7761..8360
                     /locus_tag="ACOIAF_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669221.1"
                     /translation="MYKKHKFIKNIKKAWYKIKLIDRCLIIVMAILMFQSIYNLFVNE
                     VNSQDTTTIDVVVRTTSAAIFGYFLSANFIKRPLRKYTNTDVSSNLFIDNNLSPKGQE
                     SSQNNIMNVKNTIGFTSEADNYEKKIPINNNEGFEEGETSELQIIIATVICIVALTVL
                     FVVRNFTTTTSASLGTISQMRDFVSGCVGFLLGCPSKPD"
     gene            complement(8547..9419)
                     /locus_tag="ACOIAF_002213"
     CDS             complement(8547..9419)
                     /locus_tag="ACOIAF_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGZ2669222.1"
                     /translation="MNINYLYYFQTVCKYKNMTKAAESIHISQPSITLAIKELEKELG
                     FELFYRIGNKIELTPEGKIFLDKSKHFIKQFEDFQCDALDLGKKRKASLKIGIPTVLG
                     TFLLSKILPRFNVIYPDIELKIFEVPTFVGAKMIEESTLDFCIGIIDSDIYDDIDSKT
                     IYKTELYLVTNPKNELAKHPIISNYMLKNVPFVILSEGSYHYKIITKRLEKAKPNIIL
                     HSNQLSTIRYLLENDLASTILYKEIFQNTENLCSIPLERAITANIGVLWRRNQYISHS
                     MKLFIDYMASIHIN"
     gene            9836..11239
                     /locus_tag="ACOIAF_002214"
     CDS             9836..11239
                     /locus_tag="ACOIAF_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669223.1"
                     /translation="MKGSDLVKFELMDFLMNPFVLMFAAVITGILFGKIKFGKFNFGV
                     SGALFTGLFIGWLAYSLGNLIIAKGETAAGYKAATVMMGNGIISSDFFDFFLIIFVAA
                     VGLLAAKDMKAVLKKYGARFVILGVLITFIGGSMTYAMTLLSSDKGSSAYEVSGVYTG
                     ALTSSPGLAAALETAGKHAEDVSKEFEKASIKDKKEILKVVDPEGKLDVNTTTSLTQE
                     QIDKYIAYAEAGVGIGHAVAYPFGVLIVILGVNFLPKLFRMDLKEERRKYEKEMKEAR
                     DSVSGKTIPEVPFNIITFFLTCLAGYLVGGIHVFMGPLGYFTLGATGGSLIVSLVLGY
                     IGKIGVVNFRMEEKVLNILKQIGLVFFLAIVGLRYGGKVVDSIMTSGMHLALVAIAVG
                     VTAMMIGFLVGKYVFKLNWILLSGAVCGGMTSTPGLGAAVDALDSDDPAAGYGATYPF
                     ALLTKVILVIVLHKLPM"
     gene            11266..12810
                     /locus_tag="ACOIAF_002215"
     CDS             11266..12810
                     /locus_tag="ACOIAF_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HGZ2669224.1"
                     /translation="MNNEGAFEELSGRLRNKELCDLIMNADEAAKIIEDGMVIGVSGF
                     TPSGYPKAVPLAVSERAKSGEKIKLTVYSGASLGPEVDGAWSEAGIIERRLPYQTNSI
                     LRNNINKGVVDYIDMHLSHSTQFLNYGTIPKVDVAIVEALAITEEGNIIPTSGIGNSP
                     SFIKSADKVIVEINLAKPMEMEGMADIYITENPPNRKPIEINHPKDRIGTTYIPCGLD
                     KIAGVVITNMQDKTRPLGVVDEASKKISNNIIAFLREEVKAGRLSKNLLPLQSGVGSV
                     ANAVLYGLCESEFENLTCYTEVVQDSMLDLIRMGKVTMASTTSVSPSPEGLIKFEKDI
                     DFFKDKIILRPQEISNNPEIARRIGVIAMNTAIEVDIYGNVNSTHIMGSKMMNGIGGS
                     GDFARNGAITIFSTESIAKNGDISSIVPMVSHVDHTEHDVMVIVTEQGYADLRGLAPR
                     ERAIKIIENCAHPDYKEQLRDYLNRACKSGAKQTPHILDEALSWHSKFMSTGTMKKAE
                     TFKSAL"
     gene            12841..14232
                     /locus_tag="ACOIAF_002216"
     CDS             12841..14232
                     /locus_tag="ACOIAF_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HGZ2669225.1"
                     /translation="MEKAVENFEDLSKEYINGYIERARKAQREFECYTQEQVDKIVKI
                     VGKVVYYNAEYLAKLAVEETGMGVYEDKVAKNKSKAKVIYNNLKDKKSVGIIDIDRET
                     GITKVAKPVGVVAAITPCTNPIVTPMSNAMFALKGRNAIIITPHHKAIGCSTKTVEMI
                     NEELEKIGAPENLIQILDQQSRENTRNLISSADVVIATGGMGMVKAAYSSGKPALGVG
                     AGNVQCIIDRDVDIKEAVPKIIAGRIFDNGIICSGEQSVIVAEEMFDKIMDEFKNNKG
                     FIVRDKVQKEAFRNAMFVNKSMNKDAVGQSVHTIAKIAGVEIPEDTKIIVIEADGPGE
                     EDIIAKEKMCPVISAYKYKSFEEGVAIAKANLNVEGKGHSVSIHSNTVKNIEYAGENI
                     EVSRFVINQCCATSAGGSFFNGLAPTNTLGCGSWGNNSISENLDYKHLINISRIAYYM
                     PENEVPTDEELWG"
     gene            14425..15921
                     /gene="abfD"
                     /locus_tag="ACOIAF_002217"
     CDS             14425..15921
                     /gene="abfD"
                     /locus_tag="ACOIAF_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyryl-CoA
                     dehydratase/vinylacetyl-CoA-Delta-isomerase"
                     /protein_id="HGZ2669226.1"
                     /translation="MALMTGAQYIESLRKLNTKVYMFGEEVKNWVDHPMIRPSINCVA
                     ATYDLAHDPEYADLMTVTSNITGEKINRFGHLHQSVDDLIKKVKMQRLCGQKTASCFQ
                     RCVGMDAFNAVYSTTFECDKAHGTNYHDNFVKYLTYIQENDLVVDGAMTDPKGDRSLS
                     PSAQPDPDMFLHIVERREDGIIVRGAKAHQTGSINSHEHLIMPTISMTEADKDYAVSF
                     AVPSDAEGVFMIYGRQSCDTRKLEEGADVDLGNKEFGGQEALVVFDNVFVPNDRIFLC
                     GEWDFSGMLVERFAGYHRQSYGGCKVGVGDVIIGAAALAADYNGANKASHIKDKLIEM
                     THLNESLYCCGIACSSEGHKTEAGNYQIDLLLANVCKQNVTRFPYEIVRLAEDIAGGL
                     MVTMPSEKDFKSDLKVGTSGMTIGEVCNKYFKASSVASTEERMRILRFLENICLGSSA
                     VGYRTESMHGAGSPQAQRIMISRQGNINQKKELAKKIAGIKKEEALNL"
     gene            16056..16331
                     /locus_tag="ACOIAF_002218"
     CDS             16056..16331
                     /locus_tag="ACOIAF_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669227.1"
                     /translation="MTCGVRFCGGCNPRYERGKALTIIKEHFNGRVDFSIAQENIEYD
                     LLLIIGGCATLCASYQQYKVKNECVKMWDFSHIERVINVIESAICET"
     gene            16355..17662
                     /locus_tag="ACOIAF_002219"
     CDS             16355..17662
                     /locus_tag="ACOIAF_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HGZ2669228.1"
                     /translation="MSWQELYQSKLCSATEAVKQIKNGDTVVFAHCVGEPPALVEAMI
                     ENAEQYKDVEIKHMVSLGSGGYTAKGMEAHFRVNPMFVSGNVRKAIENGDGDFTPAFF
                     HEVPKLLREKRLKCDVVLAQVTPPDEHGYCSLGTSVDYTYEAIKTARTVIVQVNDQFP
                     RTYGEVVHVSEFDYIVEKSQPLFELQPAKIGEVEEAIGKNCASLIEDGSTLQLGIGGI
                     PDAVMLFLTDKKDLGIHSEMISDGTLALYEKGVINGKYKNFDKEKMTVTFLMGTKKLY
                     DFANNNPAVEVKPVDYVNHPAIIMKQHKMVSINSAIQVDLMGQVVAEAMGLRQFSGVG
                     GQVDFIRGVSMGEDGKAIIAMPSITTKKDGTVISKIVSIVDEGAPITTSRNDVDYIVT
                     EYGIAELKGKSLRERARNLINIAHPSVREHLAVEFEKRFKEKY"
     gene            17682..18800
                     /locus_tag="ACOIAF_002220"
     CDS             17682..18800
                     /locus_tag="ACOIAF_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyrate dehydrogenase"
                     /protein_id="HGZ2669229.1"
                     /translation="MEALRVVPKIFYFDTFKEFNEEFKIGKNDLVITNEFIYEPYMKP
                     LGIDTNLIFQEKFGTGEPSDEMIDSMTKEMKKYNFDRIIAFGGGTIVDICKILALDVP
                     EKSIDLFEGEVSPKKVKELVVVPTTCGTGSEVTNVAIAELKSKHTKKGLAVEETYADY
                     AVLIPETVKGLPYKFFVTSSVDALIHAIESYLSPKASPFTEMYSLQAIKMIMDGYKKI
                     VDKGEEERFNHLRDFVLASNYAGIAFGNAGCAAVHALSYSIGGAFHVAHGEANYQFFT
                     EVFKMYSRKKPNGKIIKCTKILADALECDPNCDVYGELEKFLNKLIAKKALREYGMVE
                     SQIDEFTDSTIANQQRLLANNYVELSREEIREIFANLY"
     gene            19087..20007
                     /locus_tag="ACOIAF_002221"
     CDS             19087..20007
                     /locus_tag="ACOIAF_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HGZ2669230.1"
                     /translation="MYKSENFSKKSVLINSFLSKFLKIIGWGLVSLFTLSALVLSTIS
                     IIIGWAVILPIQVCAVFLLIGGVCIFKGKRLGDQISRYKKYCTIINNRNIIPVELIAE
                     NTSKSLNFIIKDVQKMIDKNYFINTYIDKRNNQIVLTNEDFVPPRYEDVAYEVKEEEK
                     TDESNEVDTIINKGMNYLTQIKEANKNIKSESMGDKIVQVEDVTSKIFDVVKHDPSKL
                     TQIQKFMDYYLPTTLKLLNSYHTLEEQGIDRENITTTMESIENTMNTIVVAFENQLDY
                     LFEDEAIDISTDITVLENMLVQEGLTSGKF"
     gene            20122..21279
                     /locus_tag="ACOIAF_002222"
     CDS             20122..21279
                     /locus_tag="ACOIAF_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HGZ2669231.1"
                     /translation="MNNLDDIPVMPTLTLDPFGEESNTNDIDNSDLLMKKDEKDPEEE
                     KLSESERKMVKEFAEKIDITNTNMILQYGAGAQKKVASFSETALKSVKTRDLGEVGDM
                     LTNLVTDLKAFSADEKEQSGFLGIFKKANNKISNLKTKYDSAEVNVDKVSKELQKHQV
                     KILKDIAMLDKMYELNLAYSKELTMYIIAGKQKLKDMKEYEMPKLREKARLSGSTEDA
                     QSVNDMVSLCDRFEKKIHDLELTRMVSLQMAPQIRLVQNSNNLMAEKIQSTIVNTIPL
                     WKNQIVLALGISHLNQAMKAQREVSNMTNELLLKNAETLKMGTIETAKESERGIVDID
                     TIKKTNQSLISTIDEVVKIQHDGRIKRQEAEVELSKIENELKSKLLEFTVK"
     gene            complement(21364..22863)
                     /locus_tag="ACOIAF_002223"
     CDS             complement(21364..22863)
                     /locus_tag="ACOIAF_002223"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HGZ2669232.1"
                     /translation="MAFYFDTPSHTFSEYLLVPGFSSTDCIPANVSLKTPVTKFKKGE
                     EADIYMNIPLTSAIMQSVSDDKMAIALAKEGGISFIYGSQTIENEAAMVARVKSHKAG
                     FVVSDSNIKPDNTLKDILDLKEKTGHSTVAVTEDGTSTGKLLGIVASRDYRISRMDLD
                     TKVSEFMTPMSSIVYANKDVTLKEANNIIWDHKLNSLPVLDDNGNLMYMVFRKDYSSH
                     KENPLELLDSSKRYVVGAGINTRDFAERVPALVEAGADVLCIDSSEGFSEWQKITLDF
                     IREKYGDTVKVGAGNVVDREGFLYLAEAGADFVKVGIGGGSICITREQKGIGRGQATS
                     IIEVAQARDEYFEKTGIYIPICSDGGIVYDHHITLALAMGADFIMLGRYFSRFDESPT
                     NKVNINGSYMKEYWGEGSARARNWQRYDLGGDKKLSFEEGVDSYVPYAGSLKDNVTLS
                     LSKVRSTMCNCGALSIPELQKNAKLTLVSSTSIVEGGAHDVMLKDNRNL"
BASE COUNT         8905 a         2728 c         4370 g         7116 t
ORIGIN      
        1 aataatatat ctttaaaaaa ataagataaa ttgtaaaatg atataatttt gagaaaaact
       61 caaatgcata tacacaaatt aaataacagg aggtgcaagt atgcgtcttg aattagggaa
      121 aatctttata aaagatattc aatttggtga tgtaacagaa gtaaaagatg gagtattatt
      181 catcaataag caagagatgc tacaagaaat tggtggagat gagcatatta aatctataga
      241 tatagaatta gctcgtccag gtgaaagcgt aagaataaca cctgtaaaag atgttattga
      301 gccaagagta aaagttgaag gtaatggggg aatcttccct ggtatcatgt ctaaagttga
      361 cactgtagga gaaggaaaaa ctcatgcttt aaaaggtgta gctgttgtaa ctactggaaa
      421 aatagtaggt ttccaagaag gtataataga catgactgga gaaggagcta agtatactcc
      481 attttctaaa ttaaataatg tagtagtaat agctgaacct attgatggtt taaaacaata
      541 cgctcatgaa aaagctgtta gaatgatagg attcaaagca gcaatgtact taggagaagt
      601 agcaagaaat cttactccag atgaagtttc agtatatgaa actaaacctc ttcttgaatc
      661 aataaaagaa tatccagagc ttccaaaagt aggatatgtt tacatgcttc agactcaagg
      721 tttattacat gatacttatg tatatggagt agatgcaaaa caaatagtac caactttatt
      781 atatccaaca gaattaatgg acggagcaat tgtaagtggt aactgtgttt ctgcatgtga
      841 caaaaaccca agttatgttc atataaacaa tggggttgtt gaagacttat atgcaagaca
      901 tggtaaagat ataaactttg taggtgtaat tataactaat gaaaatgttt atctagcaga
      961 taaagaaaga tcttcaaact ggacttctaa attatgtaaa tatttaggat tagatgcagt
     1021 tatagtttca caagaaggtt ttggaaatcc tgatactgac cttataatga actgtaagaa
     1081 gatagaaatg caaggtgtta aaacagttat agttacagat gaatatgctg gacgtgatgg
     1141 aggttctcaa tcacttgcag atgcagatgt aagagctgat gctgttgtaa caggtggaaa
     1201 tgccaaccaa gttgtagttc ttcctaagtt agataaagtt attggacatt tagaagtagt
     1261 agatgtaata gctggtggtt ctgatggaag tttaagagct gatggaacta ttgaagttga
     1321 aatccaagct ataacaggtg caactaatga aacaggattt ggtcatttaa ctgctaaagg
     1381 atattaagaa gtattttatt aaacgattga gtaatgttaa taaaacatat tatagataat
     1441 tataataagt gttatagata tataaaaata tattagaaat ataataagga ggattattat
     1501 gagtttactt agtaataaaa aggttcttat aataggtgac cgtgatggta taccaggacc
     1561 tgcgatagaa gaatgtgtaa aaacagtaga aggagcagag gttgttttct catctacaga
     1621 atgctttgtc tgaacagctg ctggggctat ggacttagaa aatcaaaaca gagttaaaga
     1681 tgctgctgat aaattcggag ctgaaaatgt tgtgatttta ctaggtgctg ctgaagccga
     1741 agctgcaggt cttgcagccg aaacagtaac tgctggagat ccaactttcg ctggaccact
     1801 tgctggagtt gccttaggat taagtgttta ccacgttgtt gaggaaccaa taaaatcatt
     1861 atttgatgaa agtgtatatg aagaccaaat aagtatgatg gaaatggttt tagaagttga
     1921 agaaatagaa gaagaaatgt ctggtataag agaagaattt tgtaaatttt aatgcctaaa
     1981 agcaaaaggg gtgaaactaa gagatgggaa aactaaaagc agtacattat attaatcagt
     2041 tctttgctgg tataggtgga gaagaaaaag ctgatactaa gccacatgtg gcagaaactt
     2101 tacctccaat aagtttacaa cttgataaat tattaggtga agatattgaa atagttggaa
     2161 cagtagtttg tggagatagc tatttcaatg aaaatataga tagtgcaagt gaagaagttt
     2221 tatcaatggt aaaaggtttt gaaccacaac ttttcatagc tggtcctgca tttaatgctg
     2281 gtagatatgg ggttgctgct ggaacaataa ctaaagttgt aaaagatgct ttgaatatac
     2341 cagctttaac aggtatgtat gttgaaaatc caggagcaga tatgttcaaa aaggatgttt
     2401 atgtagtaga aacttctgat tctgcagcag gtatgagaaa ggcacttcct aagatagcta
     2461 aacttgcagt aaaacttgct aatggagaag aaattggaac tcctaaagat gaaggataca
     2521 tagctagagg aataagagtt aactacttcc atgaagacag aggttctaag agagctgttg
     2581 acatgttagt taagaaaata aaaggtgaac catttgaaac tgaataccca atgccaaact
     2641 ttgatagagt tgacccaagt aaagctgtaa aagatttatc aaaatgtaaa atagctttag
     2701 ttacttctgg tggtatagtt ccaaaaggaa atccagatag aatagagtct tcttcagctt
     2761 ctaaatatgg aacttattct atagcaggag ttatggattt aacagaagag acttatgaaa
     2821 ctgcacatgg tggatatgac ccagtgtatg caaatttaga tgcagataga gtattacctg
     2881 ttgatgttct tagagattta gaaaaagaag gtgtaattgg aaaattacat gagactttct
     2941 atacaactgt aggtaatggt acatctgttg caaactctaa aaaatatgct tctgaaattg
     3001 gagcagcatt agtggctgat ggtgtagatg cagtaatatt aacttctaca tgaggtactt
     3061 gtactcgttg cggtgcaacg atggtaaaag aaatagaaaa aactggactt ccagtagttc
     3121 atatgtgtac agtagtacct atttcattaa cagtaggagc taatagaata gttccaacta
     3181 tagcaatacc tcatccactt ggaaatccag cgcttgaccc tacagaagaa aaagccctaa
     3241 gaagaggcct tgtagaaaaa gcattaaatg ctttaactac agaagtagat ggtcaaacag
     3301 tatttgaaaa ataaaaataa cacacacata tattatatag ataaaaatat aaacacacac
     3361 taaaaataaa aaataactat acacaaaaaa aataatatag atttcatgaa tggataaatt
     3421 ggagttattc attcatgaaa tcttaaaaaa tgccaaaata cataaacaat aatagaatca
     3481 aatatacaat aaaatacaat gattagagct tttaataagt ttaataagat taaaaaataa
     3541 gtgttcataa aatttatgga ggtgcacaaa atgacttatc cagttttaaa aggggcaggc
     3601 tatgtactta ttcatactcc agatatgata gtgcaaaatg gaagtacttg tacagttgaa
     3661 agagcaacaa atccagattc agaattttta aaagaagtaa gtaatcatat aagaagttat
     3721 gaagatgtag ttaactacat gccaaatcaa gtttatatag gaaatagaag accagaagaa
     3781 ttaagagatt taccaatgcc atggtgtgaa caaaagattg agggaactag aaatggtaag
     3841 tttggagaaa taatgcctca agatgagttc atagctttaa tgcaaataag tgatgcattt
     3901 gatttagtaa aattatcaca agaatttatt gatgaagtaa agccaaaaat cgaaaataac
     3961 taccctgaaa ttgcaccatt tgtaggtaaa ttaaagggtg atgatataga agaaggtaaa
     4021 gaattagtag caacacacat agctgaagga ttataccatg atggaaaatt tgttggttat
     4081 gtaaaaagag cacatgatgt agatgttaac ttaaatgccc atacaatgtt tgaaaatcta
     4141 gtagtaaaag catctggagt tttatctgct attcaaatgc taagacatag caaaatagac
     4201 cctgctgaaa tagattatgt tattgagtgt tcagaagaag cttgtggaga tataaaccaa
     4261 agaggtggag gaaactttgc taaatcaata gctgagatag caggacttca aaatgcaact
     4321 ggttctgaca caagaggatt ttgtgcagca ccaactcatg cattaattca agcagcagct
     4381 ttagtaaaag ctggcataca taaaaatgta atggtagtag caggtggagc aagtgctaaa
     4441 cttggaatga atgctaaaga ccatgttaaa aaaggtcttc ctgtacttga agatgttgtt
     4501 ggaggatttg cagtacttgt atctgaaaat gatggtgtaa atccagttat aagaactgat
     4561 ttaacaggta agcatactgt tggaactggt tcttcaccac aagcagttat gacagcactt
     4621 ataacttcag gtcttgatag agctaattta aaaataacag atgttgatgt gtattcagtt
     4681 gaaatgcaaa acccagatat aactaagcca gcaggagcag gagatgttcc agaagcaaac
     4741 tacaaaatga taggggcact tgctgttaaa cgtggagact tagaaaagaa agaattgaaa
     4801 gattttgttt ctaacaaagg attaccagga tgggcaccaa ctcaaggaca tataccatca
     4861 ggagctcctt atataggatt tttaatagat gatttaacta caggaaatag aaatagagct
     4921 atgatagttg gaaaaggtag tttattctta ggaagaatga ctaacttatt tgatggagta
     4981 tcttttatag ctgaaagaaa tacaggtgtt actgaagaga cttcaggaat atctaaggat
     5041 gaaattaaga agataatagc tgaatctatg aaaaaactag cattagatat gttagaagaa
     5101 taggggtgag gatatgtcaa aaaaagtaat cgcagatgta ttcttagaag tagctaatgc
     5161 aatagaaagc ggcgaatttg gcaaaaaagt aaaaattggt gttactacac ttggaagtga
     5221 acatggtgta gaaaacatgg ttaatggtgc acaattagca aaatctaatt tatttgatat
     5281 tgtgcttatt ggaccaaaag tggaaactga ccttgaagta gttgaagtta atgatgaaaa
     5341 agaaatgcat gcaaaaatgg aagaattatt agattcagga tatattgatg cttgtgttac
     5401 tatgcattat aattttccaa taggtgtatc tacagtaggt agagtaataa ctccagcaaa
     5461 aggaaaagaa atgatacttg caacaacaac aggaacaagt gctactaata gaatagaggc
     5521 aatggtaaga aatgccattt atggtattgc tacagctaaa tcaatgggta acaaatgccc
     5581 aaaagttgga atacttaatg ttgatggagc aagacaagtt gagaagtgct taaaagaatt
     5641 aaaagataat ggatatgaca tggagtttgc ggattcaatt agagcagatg gtggatgtgt
     5701 aatgagaggt aatgatttac ttgtaggtgc acctgatgtt atggttactg atacattatc
     5761 tggaaatata tttatgaaag tattttcttc atatactaca ggtggagatt atgaagctca
     5821 aggctttgga tacggacctg gagttggaga agactatgat agaaaggtac ttatagtatc
     5881 aagagcatca ggttctccgg tagtagcaaa tgctttaaaa tacgcttatg atgttgtaaa
     5941 gggagatata agtaatgttg caagaaatga atttgcaaag gttaaaaaag caaaatttga
     6001 tgacattata tcctctctta ctaaaaaaga agttaaagct gaaaaagtag aagttaaaat
     6061 gccagataag gaaattgtta ctcgtcaaat agctggtgtt gatataatgg atttagaaga
     6121 tgcagtatct gagttatgga aaaacggaat ctatgctgaa agtggtatgg gttgtacagg
     6181 tccaatagtt ttagtaaatg aagctaaagg agacttagca gttaaaactt tagttaaagc
     6241 tggatatact gcaaaataag tacatttttt atatgtaaat aagaacatat agttataatt
     6301 ttattgatta taattatatg ttctttttat tttatatctt gtcaatattt atatattaac
     6361 acaatccatt tatttgattc tctagctaat ttattatatt tattcaatta aaattaagct
     6421 ttctaactaa ataaaaacta atgaagtatt aaaatgaaat taataaaata tgaaaaaatt
     6481 ttaggaataa agtatctgtt agataaaaat aagattaatt agtgatataa tataattaag
     6541 ataaaattta ttatttggag gtaaaatcat gagcagagta aaaaatgtag tagaattgtt
     6601 ggaaactaaa ggtgttgatg cactatatct aacaaagaaa actaatgtaa actatataag
     6661 tggttttcct gatgaagaag catatgcagt gatttgcaaa gatggaaact ttttagttac
     6721 tgatagtaga tatatggagc ttgcagaaaa ggtttgcaaa gattttgaaa ttataaattg
     6781 gcataatttt gatagaagtg tggcaaaggc agttaaaagt gtatgtgata aagtgggtat
     6841 aaagaaactt ggatttgaaa gaactaatat agtgtttgat aaatatgaag aacttaaaaa
     6901 tctgattgag aaggataatg gagaattaat acctacagaa aatattgtag agactttgag
     6961 atatgtaaaa gacaaagatg aaatcaaaaa tacaagaaaa gcttgtgaaa tagcggataa
     7021 ggcattagaa gaattgattc cacatataaa agctggagtt agcgaaattg aactggctac
     7081 aaggttagag tactttatga aaatgaacgg agcacaaaat atagggtttg aaactatact
     7141 gatatcagga gcaaagactt cattgcttca tggtaagcct agtgataaga ttatagaaaa
     7201 aggtgatttt gtacttatag actatggtgc tatgtataat ggctatatat ctgatacaac
     7261 tagaactttt atagttggag gagcttctga aaagcaatta gaaatatata atttagttaa
     7321 ggaagctcaa aatgtagggg ttgagaacat gaaagctggt gtacatgcga caattccaga
     7381 tgctgagata agaaaagttg tcaaaaaata tgaagactat tactatcaag gtataggaca
     7441 tggagtcggt agagatgttc atgaagaacc tttcattggt aattatggtg ataaaatcat
     7501 agaagaggga tgtataataa caatggaacc aggtatttat ttccctggat ggggtggagt
     7561 tagaatagaa gatactgttt tgattactaa aaatggtcca gaaagactta ctaaattccc
     7621 taaagatttg atgattttag acaaataatt taatttatat atattttttt acataaaata
     7681 taacataatc tcactattaa gctctattta catattatag taaagagggc ttaattttat
     7741 tgtgagaggt gatagtatat atgtataaaa aacataaatt tataaaaaat ataaaaaaag
     7801 cctggtataa aatcaaatta attgatagat gccttataat tgtaatggca atattaatgt
     7861 ttcaatctat atataattta tttgttaatg aagtaaattc acaagatact acaactattg
     7921 atgtagtagt aagaaccact tcagcagcta tatttgggta ttttttaagt gctaatttta
     7981 taaagagacc tttaaggaag tatacaaata ctgatgtatc ttcaaatctt tttatagata
     8041 ataatttatc cccaaaaggt caagagtcta gtcaaaataa tataatgaat gtcaaaaata
     8101 ccattggatt tacttcagaa gctgataatt atgaaaagaa aatacctatt aataataatg
     8161 aaggttttga agaaggagaa actagtgaat tgcaaattat tatagctact gtaatttgta
     8221 tagtagcatt aacagtactt tttgttgtaa gaaattttac aactacaaca tcagcttctt
     8281 taggtactat ttcacagatg agagattttg tctcaggatg cgtgggtttt ttgctaggtt
     8341 gtccaagtaa acctgattaa aaaagtatta tcaataaaag gtattttaaa aagtatcagt
     8401 ataataatat ttagatttaa atgtatacaa ttataattct ataacattct atattgtatt
     8461 tttatactga tactctaaac tatactattt attaaaatat tttaacattg tatttaaaca
     8521 aaatattata tctgtataat ataaatttag tttatatgaa tagatgccat ataatcaata
     8581 aaaagtttca tactatgaga tatgtattga tttcttctcc aaagaacccc aatatttgca
     8641 gtaattgcac gttctaatgg tattgaacag agattttctg tattttgaaa tatttcttta
     8701 tacaatattg ttgaagccaa gtcattttct agtaaataac gtatggtaga aagttgattt
     8761 gagtgtaaaa ttatatttgg cttagctttt tcaagtcttt ttgtaataat cttatagtga
     8821 taagaacctt cagataagat tacaaaaggt acatttttta acatataatt cgatataatg
     8881 ggatgttttg ccaattcatt tttgggattt gtaactaagt ataattctgt tttatagatt
     8941 gtctttgaat ctatatcatc ataaatatca ctgtcaatta taccaataca aaaatcaagt
     9001 gtagattctt caatcatttt agctccaaca aatgttggaa cttcaaaaat tttaagctct
     9061 atatctggat aaattacatt aaatctagga agtatttttg aaagcaaaaa cgtacctaaa
     9121 actgtaggaa tacctatttt gagagaagct tttctctttt ttcctaaatc aagtgcgtca
     9181 cattgaaaat cctcaaattg ttttataaaa tgttttgact tgtctaaaaa aatttttcct
     9241 tcaggcgtta actctatttt attacctatt ctgtaaaaaa gttcaaagcc aagttctttt
     9301 tctaattctt taattgctaa tgttatggat ggttgagaaa tatgaatact ttccgcagcc
     9361 ttagtcatgt ttttatattt acagacagtt tgaaaatagt ataaatagtt tatattcatt
     9421 tggtattagt tcctttcatt tacatattag tgacaagtat ataattaaca atagcataag
     9481 ttttgtatat tgttgtaaaa aaatagaata gttttaaact atcatagcat aagtttttga
     9541 tattttcaat ctattaagaa tagtgctatt attaaattaa caaaagagaa tagctggtat
     9601 ggtttagagt aaatcaatct atattacagt tatatcaacg aatatcaaaa aattatctat
     9661 tagaaaataa aaattatttt tacaattaaa ttgttaatta aatcataaag aaaaaagtga
     9721 tattaacaag ataaaatttt tttaatcaga aatgttaatt taataacaaa atagagtatt
     9781 ggggagaaag gagaaatata ttttttaata atatttgtta atttaataac gaaaaatgaa
     9841 agggagtgat ttagtgaagt ttgaattgat ggatttttta atgaatcctt ttgtccttat
     9901 gtttgctgca gtaattacag gaattttatt tggaaaaatt aaatttggga agtttaattt
     9961 tggggtatca ggagcattat ttacaggatt atttattgga tggcttgcat atagcttggg
    10021 gaatctaata atagcaaaag gggaaacagc agcaggatat aaggcagcaa ctgtaatgat
    10081 ggggaatgga attatttctt ctgacttctt tgatttcttt ttaataatat ttgttgcagc
    10141 agttggtcta ttggcagcaa aagatatgaa agctgtatta aagaagtatg gagcaagatt
    10201 tgtaatttta ggagttctta taacttttat aggtggctct atgacttatg caatgacttt
    10261 actatcaagt gataaaggaa gtagtgctta tgaagtttct ggcgtttaca caggagcatt
    10321 aacaagttcg cctggattag ctgctgcttt agaaacagct ggtaaacatg cagaagatgt
    10381 atcaaaagaa tttgaaaaag cttcaataaa agataaaaaa gaaattttaa aagtagtcga
    10441 cccagaaggc aaattggatg tgaatacaac tacatcactg actcaagagc aaatagataa
    10501 gtatattgca tatgctgaag ctggtgttgg tataggtcat gcagtcgcat atccttttgg
    10561 agttttaata gtaatcttag gtgtaaattt ccttccaaaa ttatttagga tggacctaaa
    10621 agaagaaaga agaaaatatg agaaggaaat gaaggaagca agagatagtg tttctggtaa
    10681 aacgatacca gaagttccat ttaatataat aacatttttc ttgacatgtt tagctggata
    10741 cttagttgga ggaatacatg tatttatggg accactaggg tacttcacac taggtgcaac
    10801 aggaggttca ctaattgtat ctctagttct tggatatata ggtaagatag gggtagtaaa
    10861 tttcagaatg gaagaaaagg ttttaaatat tttaaaacaa atagggctag tatttttctt
    10921 agcaatagtt ggattaagat atggtggaaa agtagttgat tcaattatga catctggtat
    10981 gcacctagcc ttagtagcaa tagcagtagg tgttacagct atgatgatag gattcttagt
    11041 tggcaaatac gtatttaaat taaattggat tttattatct ggagctgtct gtggtggtat
    11101 gacatcaaca ccaggtcttg gagctgctgt tgatgctctt gatagtgatg acccagcagc
    11161 aggttatggt gctacatatc catttgcatt attaactaaa gttatattgg ttatagtttt
    11221 acacaaactt ccaatgtaaa taagatttta tatgggggga tatttatgaa taatgaagga
    11281 gcatttgaag aattaagtgg aagattaaga aataaagaat tgtgtgattt aataatgaat
    11341 gcagatgaag ctgctaaaat tatagaggat ggcatggtta ttggagttag tggatttaca
    11401 ccttcaggct atccaaaagc agtaccatta gcagtttcag aaagagctaa aagtggagaa
    11461 aagataaaat taaccgtata ttcaggagca tctttaggtc cagaagttga tggagcatgg
    11521 tcagaagcag gtataattga aagaagactt ccttatcaaa ctaattcaat acttagaaat
    11581 aatattaata aaggtgttgt tgattatata gatatgcacc taagtcattc tactcaattt
    11641 ttaaattatg gaactatacc taaagtagat gtagctatag tagaagcact tgcaataact
    11701 gaagaaggga atattatacc aactagtggg ataggtaatt ctccatcttt tataaaaagt
    11761 gcagataaag taatagtaga gataaatctt gcaaaaccaa tggaaatgga aggtatggca
    11821 gatatatata ttacggaaaa tccaccaaat agaaaaccaa tagaaataaa tcatcctaag
    11881 gatagaattg gaacaacata tataccttgt ggtttggata aaattgcagg agtagtaatt
    11941 accaatatgc aagataagac ccgtccattg ggagttgtag atgaagcttc gaaaaaaata
    12001 tcaaataata ttattgcttt tttaagagaa gaagtaaagg caggcagatt atcaaagaat
    12061 ttacttcctc ttcaatctgg tgtaggaagt gtagcaaatg cagtattata tggattatgt
    12121 gaatctgaat ttgaaaattt aacttgttat acagaggttg tacaggactc tatgttggat
    12181 ttaataagaa tgggtaaagt tacaatggca tctactacat ctgtaagtcc atctccagaa
    12241 ggattaataa aatttgaaaa agatattgat ttttttaagg ataagattat attaagacct
    12301 caagaaatta gtaacaatcc agaaatagcc agaagaattg gtgtaattgc aatgaacact
    12361 gcaattgaag ttgatattta tggaaatgta aattctactc acataatggg gtcaaagatg
    12421 atgaatggaa ttggtggttc tggtgatttt gctagaaatg gagcaataac tatatttagt
    12481 actgagtcaa ttgcaaaaaa tggtgatata tcatcaatag tacctatggt atctcacgta
    12541 gaccacactg aacatgatgt aatggtaatt gtaacagaac aaggatatgc tgatttaaga
    12601 ggtcttgcac ctagagaaag agctataaaa ataatagaaa attgtgccca tccagattat
    12661 aaagaacaac ttagggatta tttaaataga gcttgtaaaa gtggagcaaa acaaacacct
    12721 cacatattgg acgaagcttt atcatggcat agtaaattta tgagtacagg tacaatgaaa
    12781 aaggctgaaa cttttaaaag tgcattataa aatatttaat aaattggagg tagattttaa
    12841 atggaaaaag cagtggaaaa ctttgaagat ttaagtaagg aatacattaa tggttatatt
    12901 gagagagcaa gaaaagctca aagagagttt gaatgttata ctcaagaaca agtagataaa
    12961 atagtaaaaa tagttggaaa agtagtgtat tataatgctg aatatttagc taaacttgct
    13021 gtagaagaga ctggaatggg agtatatgaa gataaggtag ctaaaaataa aagcaaagca
    13081 aaagttatat ataacaattt aaaagataaa aaatcagttg gtataattga tatagataga
    13141 gaaactggaa taacaaaggt agctaaacca gttggagtag tagcggcaat aactccttgt
    13201 acaaatccaa tcgttacacc tatgagcaat gcaatgtttg cacttaaggg aagaaatgca
    13261 attattatca cacctcatca taaagcaatt ggatgtagta caaaaactgt agagatgatt
    13321 aatgaagaat tagaaaaaat aggagctcca gaaaatttaa tacaaatact tgaccaacaa
    13381 tcaagagaaa atacaagaaa tttaatttca tcagcagatg tagtaattgc cactggagga
    13441 atgggaatgg taaaagctgc gtactcaagt ggaaaaccag cacttggtgt aggggcagga
    13501 aatgttcaat gtatcataga tagagatgta gacatcaaag aagcagtacc taaaatcata
    13561 gcaggaagaa tttttgataa tggtatcatt tgttcaggtg aacagtcagt aatagtagca
    13621 gaagaaatgt ttgataaaat aatggatgaa tttaaaaata acaaaggttt tatagttaga
    13681 gataaagtac aaaaagaagc tttcagaaat gctatgtttg taaataaatc tatgaataaa
    13741 gatgcagtag gtcaatcagt tcatacaatt gcaaaaattg caggtgttga gataccagaa
    13801 gatacaaaaa taatagtaat agaagctgat ggaccaggag aagaggatat tattgcaaaa
    13861 gaaaaaatgt gtccagttat atctgcatac aagtataaaa gttttgaaga aggtgtagct
    13921 attgcaaaag caaatttaaa tgtagaagga aaaggacata gtgtatctat acattcaaat
    13981 acagttaaaa atatagaata tgcaggagaa aatatagaag tatcaagatt tgttattaat
    14041 caatgttgtg ctactagtgc tggaggaagt ttctttaatg gtcttgcacc tactaatact
    14101 ctaggttgtg gttcttgggg aaataacagc atctctgaaa atcttgatta taagcaccta
    14161 attaatattt ctagaattgc ttattatatg ccagaaaatg aagttcctac agatgaagaa
    14221 ctttggggat aaaatttaga caaaaaatat tcattctaaa ttaaagtagg aatatatttt
    14281 aatttagaat gaatttaaga cataaattaa atttaaatta gtttattgga ctaaatttaa
    14341 aatattaaga gacatataga agaaggaagt attataattt aaaataaata aaaaaatcta
    14401 taaaagagaa ggagtggttt tattatggca ttaatgacag gagctcaata tattgaaagt
    14461 ttgagaaaat taaatactaa ggtttatatg tttggtgaag aggttaagaa ttgggttgac
    14521 catcctatga ttagaccatc tataaattgt gtagcagcaa cttatgactt agctcatgac
    14581 ccagaatatg cagatttaat gacagttact tcaaatatca caggtgaaaa aataaataga
    14641 tttggtcatc ttcatcaaag tgttgatgat ttgataaaaa aagtaaaaat gcaaagacta
    14701 tgtggtcaaa aaacagcttc ttgtttccaa agatgtgttg gtatggatgc gtttaatgca
    14761 gtgtattcaa caacatttga gtgtgataaa gcccatggaa ctaattatca tgataatttt
    14821 gtaaaatact taacttatat acaagaaaac gatttagtag ttgatggagc tatgactgac
    14881 cctaagggag atagaagtct atcacctagt gctcaacctg acccagatat gttccttcat
    14941 atagtagaaa gaagagaaga tgggataatt gtaagaggag caaaggcaca tcaaactggt
    15001 tcaataaatt cacatgagca ccttataatg cctactataa gtatgactga agcagataaa
    15061 gactacgctg tatcatttgc agttccttca gatgctgaag gagtgtttat gatttatgga
    15121 agacaatctt gtgacactag aaaattagaa gaaggtgcag atgttgattt aggaaataag
    15181 gagtttggag gtcaagaagc tttagtagta tttgataatg tatttgttcc aaatgataga
    15241 attttcttat gtggagaatg ggatttctct ggtatgttag tagagagatt tgcaggatac
    15301 catagacaaa gttatggtgg atgtaaagta ggagtaggtg atgtaataat aggagcagca
    15361 gctttagcag ctgattataa tggagcaaac aaagcttcac atataaaaga taaactaata
    15421 gaaatgactc accttaatga aagcttatat tgctgtggta tagcatgttc atcagaaggt
    15481 cacaaaacag aagctggaaa ctatcaaata gatttacttc ttgcaaatgt gtgtaaacaa
    15541 aatgtaacta gatttcctta tgaaatagta agacttgcag aagatatagc tggagggctt
    15601 atggttacta tgccttcaga aaaagacttt aaatcagacc ttaaagttgg aacaagtgga
    15661 atgactattg gcgaagtatg taacaagtat tttaaagctt cttcagtggc ttcaacagaa
    15721 gaaagaatga gaatattaag attcttagaa aatatatgcc ttggttcatc tgctgttggt
    15781 tatagaactg agtcaatgca tggggcaggt tcaccacaag ctcaaagaat aatgatttca
    15841 agacaaggaa acataaatca aaagaaagaa ttagctaaga aaattgcggg aataaaaaaa
    15901 gaggaagctt taaatttata aaaagttgat atataaaggg aaattatata ttaatgtgta
    15961 ttaattaatg aaaaaatatg tagtataaga gatagttata aaattatggt ggtttttagc
    16021 taagtaaaga aatttttaat aaggagaatt tacacatgac ctgtggagtt agattttgtg
    16081 ggggctgtaa ccctagatac gaaagaggga aggctcttac aataatcaaa gagcacttca
    16141 atggtagggt agattttagt atagcacaag agaacatcga gtatgatttg ctacttataa
    16201 ttggaggatg cgcaactcta tgcgcatcct atcaacaata taaagtgaaa aatgaatgtg
    16261 ttaagatgtg ggattttagc catatagaaa gagttataaa tgtgattgaa tcagcaattt
    16321 gtgaaactta aaaaagctaa atggggggat ttcattgagt tggcaagaat tgtatcaaag
    16381 taaattatgt agtgctacag aagctgtaaa acagattaaa aacggtgata ctgtagtatt
    16441 tgcacattgt gtaggtgaac ctcctgcact agtagaggct atgattgaaa atgctgaaca
    16501 atataaagat gttgagatta aacatatggt aagcttgggt agtggtggat atactgctaa
    16561 gggaatggaa gctcatttta gagtaaatcc aatgtttgta agtggaaatg taagaaaggc
    16621 aattgaaaat ggagatggtg attttacacc tgcattcttc catgaagtac caaagttgct
    16681 tagagaaaaa agattaaaat gtgatgttgt tttagcacag gtaactccac cagatgaaca
    16741 tgggtattgt agcttaggaa catcagttga ttatacttat gaagccatta aaactgctag
    16801 aacagtaatt gttcaagtga atgaccagtt tcctagaacc tatggtgagg tagtccatgt
    16861 atcagagttt gactatatag tggaaaaatc acaaccttta tttgaactac aacctgcaaa
    16921 gattggagaa gtagaagaag caattggtaa aaattgtgct tcattaattg aagatggttc
    16981 tactttacag ctagggattg gtgggatacc agatgcagtt atgttatttt taactgataa
    17041 aaaggattta gggattcatt cagaaatgat ttcagatggg actttggccc tttatgaaaa
    17101 aggtgttata aatggtaaat ataaaaattt tgataaagaa aaaatgacag ttaccttctt
    17161 aatgggtact aaaaaattat atgactttgc aaataataac cctgcagtag aggtaaaacc
    17221 agtagactat gtgaatcatc cagcaattat aatgaaacaa cataagatgg tttctataaa
    17281 ttctgctata caagttgatt taatgggaca ggtagttgca gaggctatgg gacttagaca
    17341 atttagtggt gttggaggtc aagttgactt tatacgtgga gtgtctatgg gtgaagatgg
    17401 aaaggcaatt atagctatgc cttcaataac tacaaaaaaa gatggtacag taatttcaaa
    17461 aatcgtatct attgtggatg aaggtgcacc tataacgaca tcaagaaatg atgttgacta
    17521 tattgtaaca gaatacggta tagcagaatt aaaaggaaaa tctcttagag aaagagcaag
    17581 aaatttaata aatatagctc atccaagtgt aagagaacat ctagcagtag aatttgaaaa
    17641 gagatttaag gagaaatatt aataatagag gggtgttaat aatggaagca cttagagtag
    17701 ttccaaagat attttacttt gatacattta aagaatttaa tgaagaattt aaaataggaa
    17761 aaaatgattt agtaattact aatgagttta tatatgagcc atatatgaaa cctcttggaa
    17821 tagatacaaa tttaattttt caagaaaagt ttggaacagg tgaaccatct gatgaaatga
    17881 tagactccat gacaaaggaa atgaagaaat acaattttga tagaattatt gcatttggag
    17941 gaggaacaat agttgatata tgtaagatat tagcacttga tgttccagaa aagtctattg
    18001 atttatttga aggagaagta tcacctaaaa aagtaaaaga gttagtagta gttcctacaa
    18061 catgtggtac aggtagtgaa gtaacaaatg tagctattgc agagttaaaa tctaaacata
    18121 ctaaaaaggg gcttgcagta gaagaaacat atgcagacta tgcagtgtta attccagaga
    18181 cagtaaaagg gttaccttac aaattctttg ttacaagttc ggtagatgct cttatacatg
    18241 ctattgagtc ttatctttct ccaaaagcaa gtccatttac agagatgtat tctctacaag
    18301 caataaaaat gattatggat gggtataaga agatagttga taaaggagaa gaagagagat
    18361 ttaatcatct tagagacttt gtacttgcat caaattatgc aggaattgct tttggaaatg
    18421 ctggttgtgc agcagttcat gcactatctt attcaatagg tggagctttc catgttgcac
    18481 atggtgaagc aaattatcag ttctttactg aagtatttaa gatgtattct agaaaaaaac
    18541 ctaatggaaa aattataaaa tgtacaaaaa ttttagcaga tgcccttgaa tgtgacccta
    18601 actgtgatgt ttatggagaa ttagaaaagt ttttaaataa attaatagct aaaaaagctt
    18661 tgagagaata tggaatggtg gaaagtcaga tagatgaatt tacagatagt acaatagcaa
    18721 atcaacaacg actacttgct aataattatg tagagcttag tagagaggaa ataagagaga
    18781 tatttgcaaa tctttattaa tacaattttt tatttatatg tatagtcaac ataaacaatg
    18841 ctaattatat ttatgtacaa ataaaaataa gggaacttaa attttaatta aagttaagtt
    18901 ctcttatttt tatatggagt taaatataag tatattttta atttaaaata tgcttatatt
    18961 taagtctatt actttatttg aaacaatttg tagaatttca aattccttga cagtaataag
    19021 taagtaattg tataatcaaa ctgcaatata ttaataatat tagtgaaaat gaaaggatta
    19081 taaaatatgt ataaatcaga aaatttttca aaaaaatctg tattaataaa ttcattttta
    19141 agtaaatttt taaaaataat tggatggggt ttggtaagct tatttacatt atcagcctta
    19201 gtactttcaa ctatttccat tataattgga tgggctgtaa tacttcctat tcaagtatgt
    19261 gcagtatttt tacttatagg aggagtttgt atttttaaag gtaagagatt aggagaccaa
    19321 atttctcgtt ataaaaaata ttgtacaatt ataaacaata gaaatattat tccagtagaa
    19381 cttattgcag aaaacacatc taaatcatta aattttataa taaaagatgt acaaaagatg
    19441 atagacaaaa actactttat aaatacttat attgataaaa gaaataatca gattgtgtta
    19501 acaaatgaag attttgttcc accaagatat gaagatgtag catatgaagt caaagaagaa
    19561 gaaaagactg atgaatccaa tgaagttgat acaattataa ataaaggaat gaattatcta
    19621 acacaaataa aagaagctaa taaaaatatt aaatcagaat caatgggcga taaaattgta
    19681 caagtagaag atgttacaag taaaatattt gatgttgtaa aacatgaccc atcaaaactt
    19741 actcagatac aaaaatttat ggattattat cttccaacca ctttaaaact attaaattca
    19801 tatcatacat tggaagaaca aggaattgat agagaaaata taacaacaac tatggaaagt
    19861 attgaaaata caatgaatac gattgtagta gcttttgaaa atcaattgga ctatctgttt
    19921 gaagatgaag ccattgatat ttctacagat attactgttt tagagaatat gttagtccaa
    19981 gaaggattaa cgagtggtaa attttaactt caatagaaaa aatattctgt tatttttaaa
    20041 caattaatta gtcataaagt ataaaatgtg tattatataa gttaatcagt actttatgaa
    20101 ggaagaggag agaaagatac aatgaataat ttagatgata taccagttat gccaactttg
    20161 acacttgacc catttggaga agaatcaaac actaatgata tagataattc tgacttatta
    20221 atgaaaaagg atgaaaaaga cccagaagaa gaaaaactgt ctgaatctga acgcaagatg
    20281 gtaaaggaat ttgcggaaaa aatagatatt acaaatacaa atatgatttt gcaatatgga
    20341 gctggtgctc aaaaaaaggt agcaagtttt tctgaaacag ctttaaagag tgtaaagaca
    20401 agagatttag gagaagttgg agatatgctt acaaatctag ttactgattt aaaagcattt
    20461 tcagcagatg aaaaagaaca aagtggtttt ttaggaattt ttaaaaaggc aaataataaa
    20521 atttcaaatt taaaaactaa gtatgatagt gcagaggtaa atgtggataa agtaagcaaa
    20581 gagcttcaaa agcatcaggt taaaatattg aaagatatag caatgcttga taagatgtat
    20641 gaattgaact tagcttactc aaaagagctt actatgtata ttatagcagg aaaacaaaaa
    20701 ttaaaggata tgaaagaata tgagatgcca aagttgagag aaaaagctcg tttatctggt
    20761 tcaacagaag atgctcaatc tgtaaatgat atggtttcac tttgtgacag atttgaaaag
    20821 aagattcatg acttagaatt aactagaatg gtatcgcttc aaatggctcc tcagataaga
    20881 ttggttcaaa atagtaataa tctgatggca gaaaagattc aatcaactat agtaaatacc
    20941 attccattat ggaaaaatca aatagtgctt gctcttggaa tttctcactt aaatcaagct
    21001 atgaaagcac agcgtgaagt tagtaatatg acaaatgagc ttttattaaa gaatgcagaa
    21061 actttgaaga tgggaacaat agaaacagca aaagaatcag aaagaggaat tgtagatata
    21121 gacacaatta agaaaacgaa tcaatctcta atatctacta ttgatgaggt agttaaaata
    21181 caacatgatg gtagaataaa gagacaagaa gcagaagttg aattatcaaa aatagaaaat
    21241 gaacttaaaa gtaagcttct tgaatttaca gttaaataaa aattatacta atataaaaag
    21301 agactgtctc aaaatagaga taagtctctt tttcattgtt taaatgatta aagtatattt
    21361 atattataaa tttctattat cttttaacat aacatcatga gctccacctt caactatact
    21421 agtagaagat acaagtgtaa gtttagcatt tttttgaagt tcaggaatgc taagtgctcc
    21481 acagttacac atagtagaac gaactttact taaagaaaga gttacattgt ctttcaaaga
    21541 accagcataa ggcacataag agtcaacacc ttcttcaaaa gaaagtttct tatctccacc
    21601 aaggtcatat ctttgccagt ttctagcacg agcagaacct tctccccaat actctttcat
    21661 ataactacca tttatattaa ctttgttagt tggactctca tcaaaacgag agaagtatct
    21721 accaagcatt ataaaatctg cacccatagc taaagctaaa gttatatgat ggtcataaac
    21781 gataccacca tcagagcaaa ttggaatata aatacctgtt ttttcaaagt attcgtctct
    21841 agcttgagca acttcaatta tagaagtagc ttgtccacgt cctatacctt tttgttcacg
    21901 agttatacat atagaaccac caccaatacc aactttaacg aagtcagcac cagcttcagc
    21961 taggtaaagg aatccttcgc ggtcaactac atttccagct cctaccttaa ctgtatcacc
    22021 atatttttca cgaatgaaat caagtgttat tttttgccac tcagaaaaac cttctgaaga
    22081 atctatacat aaaacatcag caccagcttc aactaaagca ggaacacgtt cagcaaagtc
    22141 tctagtatta ataccagctc caactacata tctcttagaa gaatctaaaa gttcaagtgg
    22201 attttcttta tgagaagagt aatctttacg gaataccata tacataagat taccattatc
    22261 atcaagtaca ggaagagaat taagtttgtg gtcccatata atattgtttg cttcctttaa
    22321 agtaacatct ttgttagcat aaactataga tgacatagga gtcataaatt cactaacttt
    22381 agtatctaaa tccatacgac ttatacgata atcacgactt gcaacaatac ccaataattt
    22441 accagttgaa gttccatctt cagtaacagc tactgtagaa tgtccagttt tttcttttaa
    22501 gtcaagtatg tcttttaatg tgttatcagg tttaatattt gaatcactta caacaaaacc
    22561 agctttgtgg gatttaacac gtgctaccat agcagcttca ttttcaatag tttgagaacc
    22621 gtatataaat gaaataccac cttctttagc aagtgcaata gccattttgt catcagaaac
    22681 agattgcata atagcagaag tcaaaggtat attcatataa atatctgctt cctcgccctt
    22741 tttaaattta gtaacaggtg tttttaaact tacattagca ggtatacaat cagtagaaga
    22801 gaaacctggt acaagaaggt attcactgaa agtatgtgat ggtgtatcaa aataaaacgc
    22861 cattttttaa tcactcctta tatttttata ctttttataa ttgatagtat taattataac
    22921 ttattcttta attttattaa agtattttgt caaatattga gatttttgaa aaatttaaac
    22981 tattttaatt aaatttgttt aatatatatt gccctatagg gtaaagttgt atattaaata
    23041 atgtttagtg tttaatagta gctaatttaa aattaatctt tagtgattca tataattaca
    23101 tttgtatagt ttacattga
//
LOCUS       DBGJFF010000059        22136 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.060, whole genome shotgun sequence.
ACCESSION   DBGJFF010000059 DBGJFF010000000
VERSION     DBGJFF010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22136
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.060"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            444..1514
                     /locus_tag="ACOIAF_002224"
     CDS             444..1514
                     /locus_tag="ACOIAF_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2669233.1"
                     /translation="MADIRLENVTKSYNKKNTVIENLNLTIEDGSFTVLVGPSGCGKS
                     TTLRMIAGLEDITSGRLKIEENDVTKTDASKRDIAMVFQNYALYPHMTVRENIEFGLK
                     NKKVEKEKREKLITEVIDVVGLREYLNVKPGNLSGGQRQRVALARAMVKNPKVFLMDE
                     PLSNLDAKLRNQMRTELIQLHQKLKSTFVYVTHDQVEAMSMADKIVIMNKGKIMQIGS
                     PKKIYQNPKNLFVAQFIGSPCMNILDIKNDIKFGFRPEKATLESINIDGKNDDVFSLF
                     GKVVTKEVLGNETIYCLSVLNREIRVKTENDIFDIGRTLRISVLEKDLYYFDKNGERI
                     EDVKVCKSMYSKIKGDIYEKAI"
     gene            1498..2367
                     /locus_tag="ACOIAF_002225"
     CDS             1498..2367
                     /locus_tag="ACOIAF_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGZ2669234.1"
                     /translation="MKKQFNKAYLYITPAIIGTLLFIVYPIIKTISLSFKSGSLLSST
                     FENVGLNNYTELLKNPEFIQTITNTAIYTFFMVTISISLAIVLAVWLNKNSIIHNLTQ
                     SIIFTPHVISLVSVAVLWLWIMEPQYGFLNWILSTLHLPTSKWLSSESSALMSLILIG
                     IWKTLGYNILIVLSGLKSIPNYIYEASKLDNANKITNFFKVTLPLLSPTIFFLMITTT
                     TASFQVFDEISIMTQGGPVGSTNMLSYYIYESGFIYYDIGQASAASMFLLAIVMIATY
                     ANFRLLSKKVHYQ"
     gene            2411..3271
                     /locus_tag="ACOIAF_002226"
     CDS             2411..3271
                     /locus_tag="ACOIAF_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGZ2669235.1"
                     /translation="MDIIQPIKKDKVDIKKIASKSIEILILLLLSAIFIFPFIWMLST
                     SFKNPQEMMQLPPTIIPNDIVLENYSTAWHSGPFFRYLLNSVFVTGSVLIVQFLVMVP
                     AAYAFSKIKFKGEKMLFGLLLVGLMIPPQVTFLPVYMMMSKFGFINTYVPLIIPFMTS
                     SFGIFLLRQNFMQISDEIIEAAKLDNASDLKIMFRIMVPMAKPAVITFILFNFIYHWN
                     NYFWPLVMTNTDAIRTLPIGVALLKSSEGITAWNVIMAGNIILILPIILVYIFANKKV
                     KEAFMYSGIK"
     gene            3302..4606
                     /locus_tag="ACOIAF_002227"
     CDS             3302..4606
                     /locus_tag="ACOIAF_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGZ2669236.1"
                     /translation="MKLKRKLATGLAITLLLGSLVGCSNSNDANASKGEKTDSNITKI
                     DFWAPLGGTNGETAQKMVDQFNSEHKDVQVNMLKQKDYYENATKLQAALTSKDQPDVT
                     LLEITQTGTFASAGALVDMSKYFDKTYQERFFSGLLTNSYYEDKFVGMPFNRSTPILY
                     INKDMATKAGLDPSGPKSWEELKDYASKMTNKSEDTYGFETPIDIWFYEAMVMQSGGE
                     ITNGKKVAFNNEAGQAPVKFWQDMMKAGIMKMPPGEDYNAWDVAKQDFVNGKVGMIFT
                     STADLAGLMQQTEGKFEISTAFLPKNQKYATPTGGANLVMLEGGTDKEKEASAEFIEW
                     MTQTDKIVQFSSSTGYLPTTEDATNSEKLQTLYKEKPQYKVATDQLQYAVALPMLNGY
                     KEATDKLMDEIKKGLTDLNANPKDVVSKGTSAMQAVIDKTNK"
     gene            4669..5931
                     /locus_tag="ACOIAF_002228"
     CDS             4669..5931
                     /locus_tag="ACOIAF_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HGZ2669237.1"
                     /translation="MTKVIVLSVDSLFEKDLEFVKNLPNFKSILENCSIVKNINCVYP
                     TLTYPCHTSMVTGVYPKKHGIYHNEKFDPNKENKDWYWYSKDIKAKTIIDVAKENNLT
                     TSSVLWPVMGANPNIDYNIAEIWAPSREDDPRETFERSSSKVIMDGIYNRHCHYIDWK
                     LEPNMDLFGVNCSVDIIKEYKPDLMLIHLATLDHTRHNYGLFNDEVDKALKMNDKWLG
                     DIIQATKDAGTYKDTNFIILGDHGHLRVDKVINPNVLLKSNGFIKVENGEVKDFDAYV
                     NSSGISAQIIVNNLDRLTELRELLEEFKEELFIENIFTKEEASNLGLEGDFEMVIEGL
                     EGISFGNDFEGSIIKDSDIKDYKFAVATHGHLPTKGNRPPFIAFGPNIKGGVVIEEGD
                     LRAHAATILKMFNLKLDGVEKEAFDFIM"
     gene            6118..8088
                     /gene="uvrB"
                     /locus_tag="ACOIAF_002229"
     CDS             6118..8088
                     /gene="uvrB"
                     /locus_tag="ACOIAF_002229"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HGZ2669238.1"
                     /translation="MDFKIKSDFKPTGDQPEAIKSIVDSINRNEKFSTLLGVTGSGKT
                     FTMANIIQQVKKPTLIMAHNKTLAAQLYSEFKEFFPDNAVEYFVSYYDYYQPEAYVAH
                     SDTYIEKDASINDEIDKLRHSATASILERRDTIIISSVSCIYGLGDPKDYKELMLSIR
                     PGMQRDRDDVIKRLIEIQYERNDINFTRGTFRVRGDILEIFPASNDEKAIRIEFFGDE
                     VDRITEIDYVTGKIVGTRNHVVIFPASHYVTTPERIEKAIVEIENELQEQIKFFKEND
                     RLLEAQRIEQRTKYDIEMLKEIGFCQGIENYSRHITGRSEGERPYTLMDFFPDDYLII
                     VDEAHVTIPQVRGMYAGDRSRKTSLIENGFRLPSALDNRPLNFQEFEGNINQMLFVSA
                     TPGPYEIQHSETIAEQIIRPTGLLDPIVEVRPINNQIDDLVGEITKTIEKNERVLITT
                     LTKKMSEDLTNYLKEIGIKVKYLHSDIVTLERTEIIRDLRLGKFDVLVGINLLREGLD
                     IPEVSLIAILDADKEGFLRSETALIQTIGRAARNENGRVIMYADRITDSMQNAIDETK
                     RRRDIQNLYNEEHNIIPKTIQKNIRDSIEATKVAEEEVVYGISDTDDKDEIRANIDKL
                     KSEMMEAAQNLQFERAAELRDKVKQLEEKLEK"
     gene            8107..10932
                     /gene="uvrA"
                     /locus_tag="ACOIAF_002230"
     CDS             8107..10932
                     /gene="uvrA"
                     /locus_tag="ACOIAF_002230"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HGZ2669239.1"
                     /translation="MEDKIIIRGAKEHNLKNIDLELPRDKFIVFTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGQMEKPNVEYIEGLSPAISIDQKTTSKNPRSTVGTV
                     TEIYDYLRLLFARVGDVYCPTCGEKISQMTIQEIVDKMLDFPDRTKLQILSPIVRGQK
                     GTHKKVFDNIKKEGFVRVRVNGENYEVSDDIILNKNQKHNIEVVVDRIVIKDGIESRL
                     ADSIETAVKLSDGLVIAQIIDGEEILFSTKFACPEHGIGIEELSPRMFSFNAPFGACD
                     VCNGLGESKEVDPELVVPNKDLSIKQGAVAAWGSTGVNDDTYYSKMIKSLAEHFNVSL
                     TTPFKDLPDDFVEELLYGKDNIIVEFTYESKFGGTRNYKSYFEGVIVNLERRYRETNS
                     DYMRDKIEEYMAERPCPKCKGMRLKKEVLSVLVDGKNIMEVTNLSVNELIDFMENINL
                     TEKQRFIAHEIIKEIKGRAMFLRDVGLDYLNLSRKAGTLSGGEAQRIRLATQIGSALV
                     GVLYVLDEPSIGLHQRDNDRLITTLRHLTDLGNTLIVVEHDEDTMREADYVIDIGPGA
                     GIHGGEIVAQGTLDEIIENENSITGQYLSGKKQILLPETTREGNGSFIEIVKASENNL
                     KNIDVKFPLGKFTCITGVSGSGKSTLINDILYKGVASKVNRLKQRAGKHKEIKGIESI
                     DKVINIDQSPIGRTPRSNPATYTGVFDFIRDIFASTNEAKARGYKKGRFSFNVKGGRC
                     EACKGDGIIKIEMHFLPDVYVPCEICKGERYNRETLQVKYKDKTISDILDMNVEEAVE
                     FFENIPNIKRKLETLMDVGLSYIKLGQPSTQLSGGEAQRIKLAAELSKRPTGKTLYIL
                     DEPTTGLHMADVDKLIQVLQRLADTGNTIIVIEHNLDVIKTCDYVIDLGPEGGDKGGT
                     IVATGTPKEVSKVDGSYTGQFLKKYFS"
     gene            11191..13008
                     /gene="uvrC"
                     /locus_tag="ACOIAF_002231"
     CDS             11191..13008
                     /gene="uvrC"
                     /locus_tag="ACOIAF_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HGZ2669240.1"
                     /translation="MFDIQEHLKKLPSEPGVYLMKDKYDHIIYVGKAISLKNRVRQYF
                     QSSKNHTSKVKSMVKNIYKFEYIITDSELEALILECNLIKRYRPKYNVVLRDDKTYPY
                     IKVTTNEDYPRILKVRRVLKDKAKYFGPYTNISAVNDTLELISSTYPIRSCKIDIDKA
                     IKNKTRPCLNLHINKCLGPCTGNVSKEEYGKMIEEIIMCLSGKEEKLMELLKEKMNES
                     SMNFRFEEAAVYRDKIKSLEEMIQKQKIDATVSDLNQDVVAMARAHNEACVQVFFIRN
                     GKIVGREHFILEGVMDSPRASILSSFVKQFYNEQEYIPKELIIEDEIEDSSILEEWLS
                     SKKGQKVTIRVPQKGEKKSLVEMVRKNAVEYLEKFSDMNKRKYEKSIGALEELKQILN
                     LEKLPIRIEAYDISNIQGVDSIGSMVVYTNAKKDKKEYRRYKIKTVIGPNDYDSMAEI
                     VDRRLKHGNLPDLILLDGGKGQVSAVKKVLELNDVDIPLWGMYKDDKHRTKGLICKEK
                     EIELDKTTNLYRFIASIQEEVHNYAITYHRSLRNKALTKSILDDIQGIGEKRKKSLLN
                     HFKDVDAIKKATMEELLEVDGMNKSIADNVYNFFRKEEN"
     gene            13008..13937
                     /gene="hprK"
                     /locus_tag="ACOIAF_002232"
     CDS             13008..13937
                     /gene="hprK"
                     /locus_tag="ACOIAF_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HGZ2669241.1"
                     /translation="METSNKVSIREIIKELKLKVVYMPDDVDYYVYSQDINRPGLQFA
                     GYFDYFAYERIQIVGKTEYNFFGLMDSHIREEVLDRFFSYEIPALIISRDLEIKPDVI
                     EKAKKYKRILLSTNRNTTRLINRLSNYLDNKLAPHTTIHGVLVDIYGIGVLIKGESSI
                     GKSETALELIQRGNRLVADDAVEIRKLDESMLVGQSPELIRHFLEIRGIGIIDVRSLY
                     GVGAIKNSKMIDLVVHLEAWDETKYYDRLGLDKEYEEILGVNVEKLVVPVKPGRNTAM
                     ILEVAAMNFRQRGMGYDAAQEFTKKLSKLIDTK"
     gene            14151..15947
                     /locus_tag="ACOIAF_002233"
     CDS             14151..15947
                     /locus_tag="ACOIAF_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate oxidoreductase"
                     /protein_id="HGZ2669242.1"
                     /translation="MGIEYFSKLLESSTEKELLLSKKSNNISTARLISFLIVIAGFAI
                     GFYNKNIVGIFVGVLSIIIFIALLVIHSKVKEEETYFKSKSEVLNKYIKRFGDGWKEF
                     KIDGKEYLKDENSQAKDLDLFGRASLYQYICVANTSYGKNFLAKYLWNENPDENVILE
                     RQRAIKELLSKDDFSIHIQTLSNIIGKEQKNNSDSSIESFIEYGENKNVYIPKWMHIF
                     TWGLPTATILSFIFCMLGFLPVLPVFLLFIIQLGFSGFGYPRLMQTLAPLFSFSRSIQ
                     VYEKMFEVLEKETFESAYLKELQDKLSKGSGVSRGVKQLNSIGNAVNLRYNQILYIIA
                     CGVLMWNYHCAEALERWKGIYGNQIRDWFESIGEFEALISLTVVSHVKENTCFPVIKY
                     EDTPRLKVEEVYHPLIAEKSVIANSIQLNSQTCIITGSNMSGKTTFLRSIGVNLVLAY
                     AGAPVCAKSFDATCMAIFTSMRIQDDVSQGISTFYAEILRIKSMIQYSLKELPMLVLV
                     DEIFKGTNSADRIIGASEAVKKLSKPWVISMVTTHDFELCDLSGSGDVEVVNYHFSEY
                     YVDDKIHFDYKMKDGRCKTTNAKQLMRMAGIL"
     gene            complement(16087..17868)
                     /locus_tag="ACOIAF_002234"
     CDS             complement(16087..17868)
                     /locus_tag="ACOIAF_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HGZ2669243.1"
                     /translation="MSLVNLTINGKHVSAPSGTSILDAAKLINIKIHNLCHLHMNEID
                     KLDTCASCRVCMVETERGLVPACGTVIKEGMKVQTNSAKALNARRTIVELLLSDHPQD
                     CFVCEKNGDCELQTIAADLGVRKIRYQGSKSFAGKDTSTKSLVKDHSKCILCRRCETV
                     CNDIQTVGALSGVNRGFNTLVSTFFNSDMVETECTFCGQCISVCPTGALTEVDNVPKL
                     WDVLNKKEKAIVVQVAPAVRVAIGEEFGLEPGSISTGKMVAALKALGFEHVFDTNFGA
                     DFTIMEEATEFIERIQKGENLPILTSCCPAWVNFLEHNYPDKLNLASSCKSPQGMFGS
                     IAKNYYAPKILGINPEELYVVSVMPCVAKKYEASREELSESGILDVDLSITTRELAKM
                     IKEAAIDLPNLQDQDFDNPLGKSTGAASIFGASGGVLEAALRTSYEKITNKTLDNVNF
                     TNVRGLKGIREASIDVDGTTVNVCIVNTLKNARKIMDKVRSGECKYHIIEVMACPGGC
                     VGGAGQPYHHGNTEIVDRRANALYEIDRNKAIRKSHENPDLQAIYKDFFGEPNSDVAH
                     KYLHTHYFDKSCVYGECPQECACEEAK"
     gene            complement(17891..19777)
                     /locus_tag="ACOIAF_002235"
     CDS             complement(17891..19777)
                     /locus_tag="ACOIAF_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HGZ2669244.1"
                     /translation="MRKVNSFDELKVLADELKPNLSLRKNYSKENVLRRELLVCCDTG
                     CTSSNSLEIVSELENEIKKSGIQDKVSVRLTGCFGFCAQGPIVKVYPDNVFYVKVEPS
                     DAEKIVQSHLIRNTVVEELLYEEESLSKKVEEQEEMSFYKKQLRIALKNCGLIDPDSI
                     EEYIANDGYLALGKCLTSLTPQEVIAEVKTSGLRGRGGAGFPTGSKWEAASKNPAGPT
                     DKKFVVCNADEGDPGAFMDRSVLEGDPHSVLEAMAICGYAIGSDTGYIYIRAEYPKSI
                     ERLKVAIAQAEKYGLLGENILGTGFNFKLELKYGAGAFVCGEGTALMHSIEGRRGEPR
                     MKTYSSSKSGLWKSPTCLNNVETFANIPAIILKGGDWFANLGTEDSSGTKVFALGGKV
                     ENVGLVEVPMGTTLRDIVFEIGGGIPEGKKFKAVQTGGPSGGCIPTEHLDTPIDFGSL
                     SSIGSMMGSGGMLVLDESDCMVDIAKFFLEFTVEESCGKCTPCRIGTTRLLEILTKIT
                     EGNGTLQDLDDLENLAETIQTASLCGLGKAAPNPVLSTLQYFKDEYIAHVVDKKCPAG
                     KCQNLLSYFITDDCKGCTKCSRVCPAGCITGSVKEQHTIDTSKCLKCGACIDNCTFNA
                     IIKK"
     gene            complement(19794..20291)
                     /locus_tag="ACOIAF_002236"
     CDS             complement(19794..20291)
                     /locus_tag="ACOIAF_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HGZ2669245.1"
                     /translation="MCDFISHNKQLFDELDIFIDSLATKEGALIQVLHEAQGIFGYLP
                     KEVQLHVARKLGVAPAKVYGVVTFYSYFTTEPVGKYKISVCLGTVCFVKGADKILSAF
                     EKQLGIKVGETTSDFKFSLEGLRCLGACGLAPVVTVNGKVYGKVKPDQVSEILDTYRE
                     LELNC"
     gene            complement(20609..22090)
                     /gene="cls"
                     /locus_tag="ACOIAF_002237"
     CDS             complement(20609..22090)
                     /gene="cls"
                     /locus_tag="ACOIAF_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HGZ2669246.1"
                     /translation="MFDISFFEMSIYEIAVTTIYIINFMVILNLIFREKRNINTTLTW
                     LLILVLIPALGFILYIAFGRNISKNNMFRLKEKDDKIIKSNILDTQVKLQSTSEIDSD
                     IHQHKDMIYALANSNNAHYTNNNDVWIYAESSQFFNSLLEELKKAKKYINIQFYIFKD
                     DKIGTEIIDILIDKAKEGVEVRLLFDAVGGRTLKNSTLSRLKESGVKVGSFFPSFLKI
                     VNFNLNYRNHRKIVVIDGKVGFVGGYNVGDEYLGRNPKFGLWRDTHTKLTGDCVIDLN
                     MRFILDWRYTTKEDLDLEKYFIEEDTNSNHPSENIGIQIVSSGPDISELDEIKYGYLK
                     MIQKARKYIYIQSPYLILDSTFIDTLKIACLSGVDVRVMIPSKPDHPFVYWASYSYAG
                     ELLKFGAKIYTYGQNAFLHAKTIVIDDSICSIGTANMDIRSFELNFEVNAFMYSSKKA
                     LEQRIIFENDILNSKEITLDIYNSRSTYIKIKESISRLLSAVL"
BASE COUNT         8513 a         2965 c         3702 g         6956 t
ORIGIN      
        1 ggtaaattta taaaaattaa aaaattttta ttttttatgt ttaaaccttg taaatttggg
       61 acataatata attgtaaaac ttaatatagc aaaaataaat tactcaatct cccccactta
      121 aagacctcta tcccccatag aggtcttttt tactttcaaa tcaaattata taaaaaataa
      181 atagttttat atgactcttc ataaattaaa attacattgt gatagcaaac catgaatatg
      241 aaaaatatag aaaattatag ctaacataag ctgtatatca tcttaacctc agtatattta
      301 acaaaaaatg tttataattt aacctttatt ttaataagtt taccatatat tcactttgtc
      361 ttaatatatc ctttacaaat taagagtatt attaatctat aagtaaatcg tagaattaaa
      421 ttaaatattt tgggaggata aaaatggcag atataagact agaaaatgta acaaaatctt
      481 ataataaaaa aaatacagtt atagaaaatt taaatcttac tatagaagat ggttctttta
      541 cagtattggt aggcccaagt ggatgtggta aatctactac gttaagaatg attgcaggtc
      601 ttgaagatat aacttcaggt aggttgaaga ttgaagaaaa tgatgttaca aaaacggatg
      661 ctagtaaaag ggatatagct atggtatttc aaaattatgc attatatcca catatgactg
      721 ttagagaaaa catagaattt ggtcttaaaa acaaaaaagt agaaaaagaa aaaagagaaa
      781 aacttataac agaagttatt gatgtagtag gattgagaga atatttaaat gtgaaaccag
      841 gcaacttatc tggaggtcaa agacaaagag tggcacttgc tagagctatg gttaaaaatc
      901 caaaggtatt tttaatggat gaaccattgt caaatttaga tgctaaatta agaaatcaaa
      961 tgagaacaga attaatacaa ttacatcaaa aactaaagtc tacatttgtt tatgtaactc
     1021 atgaccaggt tgaggcaatg tcaatggcag ataagatagt tataatgaac aaaggaaaga
     1081 taatgcaaat aggttctcca aagaagatat atcaaaatcc taaaaattta tttgtagctc
     1141 aatttatagg gagtccttgt atgaacatac tagatataaa aaatgatatt aaatttggat
     1201 tcagaccaga aaaagcaaca ctagagagta taaacataga tggaaaaaat gatgatgtat
     1261 ttagtctatt tggaaaagtt gtaactaaag aagtgttggg aaatgaaact atatattgtt
     1321 tatctgttct taatagagag ataagagtca aaacagaaaa tgatatattt gatattggaa
     1381 gaactttaag aataagtgta ttagaaaaag atttatatta ttttgataaa aatggagaaa
     1441 gaatagaaga tgtaaaagta tgtaaaagta tgtattctaa aattaaagga gatatttatg
     1501 aaaaagcaat ttaataaagc atatttatat ataacacctg ctattatagg aactttattg
     1561 tttatagtct atccaataat taagacgata tctctaagct tcaaatctgg tagtcttctt
     1621 agttcaacct ttgaaaatgt aggcttaaac aattatacag aacttttaaa gaatccagag
     1681 tttatacaaa ctataacaaa cactgcaatt tatacttttt ttatggtgac tatatctata
     1741 tcactagcca tagtacttgc tgtttggcta aataaaaatt ctataataca caatttaacg
     1801 caaagtataa tctttacacc acatgtaatt tcactagtat ctgtagctgt attgtggctt
     1861 tggattatgg agccacaata tggcttttta aactggatac tatcgacatt acatttacca
     1921 acatcaaagt ggctttcaag tgaaagttca gctttaatgt cactaattct aataggaata
     1981 tggaagactc ttggatataa tatcttaata gtgttatcag gtcttaaatc cataccaaac
     2041 tatatatatg aagcatctaa attagataat gcaaacaaaa taacgaattt ctttaaagtg
     2101 acattgccac tattatcacc aacaatattt tttcttatga taacaacaac aactgcatct
     2161 tttcaagtat ttgatgagat aagtataatg acacaaggtg ggccagtggg ttcaactaac
     2221 atgttatctt attatatata tgagtctgga tttatatact atgacatagg gcaagcgagt
     2281 gcagcgagta tgtttttgtt agctatagtt atgattgcta catatgctaa ctttagactc
     2341 ttatcaaaga aagttcatta tcaataaaga aaaagtagat attataaggt ttatccaaaa
     2401 ggaggagaca atggatatta tacaacctat caaaaaagac aaagttgata taaaaaaaat
     2461 agcatcaaaa tcaatagaaa tattaattct attactttta tctgcaatat ttatattccc
     2521 ttttatatgg atgctatcta catcctttaa aaatccacaa gaaatgatgc aattaccgcc
     2581 cactattata ccaaatgata tagtgttaga aaattatagt acagcatggc attcaggtcc
     2641 attttttaga tatttgctaa acagtgtatt tgtaacagga agtgttttaa ttgtacaatt
     2701 tttagttatg gttccagcag catatgcatt ttctaagatt aaatttaaag gtgaaaaaat
     2761 gctatttggg cttttgcttg taggacttat gattccacca caagtaactt ttctaccagt
     2821 ctatatgatg atgagtaagt ttggatttat aaatacttat gttccactta taattccatt
     2881 tatgacatct tcatttggga tatttctgct tagacagaat tttatgcaga tttcagatga
     2941 aattattgaa gcagctaaac tagataatgc ttctgactta aaaataatgt ttagaataat
     3001 ggttcctatg gcaaaaccag cagtaataac atttatatta tttaatttta tataccattg
     3061 gaataattat ttctggccct tagtaatgac aaatacagat gcaataagaa ctttaccaat
     3121 aggagttgcg ctccttaaat cttcagaagg aattactgca tggaatgtaa taatggcagg
     3181 aaatataatt ctaattttac caataattct tgtttatata tttgcgaata aaaaagtaaa
     3241 agaagctttt atgtattcag gaataaaata aaaaattaaa gactaaattt ggaggatgaa
     3301 aatgaagctt aaaagaaaat tagcaacagg attagcaata acattattat taggttcttt
     3361 agttgggtgt tcaaattcta atgatgcaaa tgcatctaaa ggagaaaaga ctgatagcaa
     3421 tattacgaag atagatttct gggctccttt aggtggaact aatggagaaa ctgctcaaaa
     3481 aatggttgac caatttaata gtgaacataa ggatgtacaa gtaaatatgt taaaacagaa
     3541 agattactat gaaaatgcaa caaaactaca ggcagcctta acatctaaag accaaccaga
     3601 tgtaacatta ttagaaataa ctcaaacagg tacattcgca tcagcaggtg ctttagtaga
     3661 tatgagtaaa tattttgata aaacatatca agaaagattc ttttctggtc ttttaacaaa
     3721 ttcatattat gaggataagt ttgttggtat gccatttaat agaagtacac caatattata
     3781 cataaataaa gatatggcaa ctaaagcagg tcttgaccct tcaggtccaa aatcatggga
     3841 agagcttaag gattatgcta gtaaaatgac aaataaatca gaagatactt atggttttga
     3901 aacaccaata gatatttggt tctatgaagc tatggtaatg caaagtggag gagaaataac
     3961 taatggtaaa aaagtagcat ttaataatga ggcaggacaa gcaccagtta agttctggca
     4021 agatatgatg aaagctggaa taatgaaaat gcctcctgga gaagattata atgcctggga
     4081 cgtagcaaaa caagattttg taaatggtaa agtaggtatg atatttacat caacagcaga
     4141 tttagcagga cttatgcaac aaacagaagg taagtttgaa atatcaacag cattcttacc
     4201 aaaaaatcaa aaatacgcaa ctcctacagg tggagctaat ctagttatgc ttgaaggtgg
     4261 aacagataaa gaaaaagaag caagtgcaga atttatagaa tggatgacac aaacagataa
     4321 aatcgttcag tttagttcat caacaggata cttaccaaca actgaagatg caacaaactc
     4381 agaaaagtta caaacattat ataaagaaaa acctcaatat aaggttgcaa ctgaccaatt
     4441 acaatatgct gttgcacttc caatgttaaa tggatacaag gaagcaactg ataagctaat
     4501 ggatgagatt aaaaaggggc ttactgattt aaatgcaaat ccaaaagatg tagtaagcaa
     4561 aggaacttct gcaatgcaag cagtaataga taaaacaaat aaataattat atagacttgt
     4621 aagttgatta aaagtattca ataatatata aatgaagagg taatgtaaat gacaaaggtt
     4681 atagtattat cagtagattc tttatttgaa aaagacttgg aatttgtaaa aaatctacca
     4741 aactttaaat ctatactaga aaactgctcc atagtaaaaa atataaattg tgtttatccc
     4801 actctgacat atccgtgtca tactagtatg gttacaggag tatatcctaa aaaacatggt
     4861 atatatcata atgaaaaatt tgacccaaat aaagaaaata aagactggta ctggtactca
     4921 aaggacataa aagcaaaaac tattatagat gtagcaaaag aaaataatct aactacctct
     4981 agtgtattat ggcctgtaat gggagcaaat ccaaatatag attataatat tgcagaaata
     5041 tgggctccta gtagagaaga tgacccaaga gaaacttttg aacgaagttc cagtaaggtt
     5101 ataatggacg gtatttacaa tagacattgt cattatatcg attggaaatt agaacccaac
     5161 atggatttat ttggagtaaa ctgttcagta gatataataa aggaatataa accagattta
     5221 atgctaatac atttagctac attggaccat acacgacaca attatggctt atttaatgac
     5281 gaagtagata aagcattaaa aatgaatgat aaatggttag gagatattat tcaggctaca
     5341 aaggatgcag gtacatacaa agatacaaat tttataatac ttggggacca tggtcatctt
     5401 agagttgata aagttataaa tccaaatgtc ttgcttaaat caaatggatt tataaaagtt
     5461 gaaaatggag aagttaagga ttttgatgcc tatgtaaatt cttctggaat atcagctcaa
     5521 ataatagtaa ataacttaga taggttaaca gaattaagag aactgcttga agaatttaaa
     5581 gaagaattat tcatagagaa tatttttacc aaagaggaag catctaacct tggtcttgaa
     5641 ggtgattttg aaatggtaat tgaaggtctt gaaggaattt catttggaaa tgatttcgaa
     5701 ggaagtatta taaaagattc agatataaaa gattacaagt ttgctgttgc gacacatggt
     5761 catttaccta ctaaaggtaa tagacctcca tttatagctt ttggaccaaa tattaaaggt
     5821 ggtgtggtta tagaagaagg agacctgaga gcacatgcag caactatatt aaaaatgttt
     5881 aatttaaaat tagatggagt tgaaaaagaa gcatttgatt ttattatgta agttgaataa
     5941 agtatttatt ttccatgaat tatagacttc tattgcaaag atagaagtct ttttttatgt
     6001 tttagattta agtaaaattt aaatgggtaa aatatttaaa ataacttaaa caattttata
     6061 aaggtatgat ataataatac atgtcaggaa ctaatgttcg aaaaaaggag tgatactatg
     6121 gattttaaaa taaaatcaga cttcaaacct acaggtgacc aaccagaagc tattaaatca
     6181 atagtggatt ctataaatag aaatgagaaa ttttcgacat tattaggtgt aacgggttca
     6241 ggaaaaacat tcacaatggc aaatatcatt caacaagtta agaaacctac cctaataatg
     6301 gctcataata aaactctagc agcacaactt tacagtgagt ttaaagaatt tttcccagac
     6361 aatgcagttg aatactttgt aagttactat gattattatc aaccagaagc atatgtagct
     6421 cacagtgaca cttatataga aaaagatgca agcataaatg atgaaataga taaacttcga
     6481 cattctgcta cagcatctat tttagagaga agagatacta taataatatc atctgtatct
     6541 tgtatatatg gacttggtga cccaaaagac tataaagaac ttatgttatc tataagacca
     6601 ggtatgcaaa gagacagaga tgatgtaata aaaaggttaa tagagataca atacgaaaga
     6661 aatgacataa actttacacg aggtaccttt agagttagag gagatatact agaaatattc
     6721 ccagctagta atgatgaaaa agcaataaga atagaatttt ttggtgatga ggtagataga
     6781 ataacagaaa ttgattatgt aacagggaag attgttggta ctagaaatca tgttgttata
     6841 ttcccagcat ctcactatgt aacaacgcca gaaagaattg aaaaggcaat tgtagaaata
     6901 gaaaacgagc ttcaagaaca aattaaattt ttcaaggaaa atgataggct attagaagca
     6961 caaagaattg aacaaagaac taagtatgat atagaaatgt taaaggaaat aggattttgt
     7021 caggggatag agaactattc tagacatatt acaggaaggt cagaaggaga aagaccatat
     7081 actttaatgg atttcttccc agatgattat ttgataattg tagatgaggc acatgtcact
     7141 ataccacaag taagaggtat gtatgcagga gacaggtcta gaaaaacatc acttatagag
     7201 aatggtttta gattaccttc tgcacttgac aatagaccat taaattttca agaatttgaa
     7261 ggaaatataa atcaaatgtt gtttgtaagt gctacaccag ggccttatga aattcaacac
     7321 tcagagacaa tagcagaaca gataataaga ccaactggcc ttttagaccc aattgtagaa
     7381 gtaagaccta taaataatca aatagatgat ttggtaggag aaataactaa aactatagaa
     7441 aaaaatgaaa gagtgcttat aaccacttta actaaaaaaa tgagtgagga cttgactaac
     7501 tatcttaaag aaatagggat aaaagttaaa tatttacact cagatatagt tacattagaa
     7561 agaacagaaa taataagaga tttaagatta gggaagtttg atgtccttgt aggaataaac
     7621 ttacttagag aaggattgga tataccagaa gtttcactta tagcaatttt agatgcagac
     7681 aaagaaggtt tcttacgttc cgaaacagct ttaatacaga ctattggacg tgcagctaga
     7741 aatgaaaatg gaagagtaat tatgtatgca gacaggataa ctgactcaat gcaaaatgct
     7801 attgatgaaa caaagagaag aagagatata caaaatttat ataatgaaga gcacaatatc
     7861 attcctaaaa ctatacagaa aaatataaga gatagtatag aagctactaa ggttgcagag
     7921 gaagaagttg tatatggaat atctgataca gatgataaag acgaaataag agctaatata
     7981 gataaattaa aatcagagat gatggaagct gctcaaaact tacaatttga aagagcagca
     8041 gaacttagag ataaagtgaa acaattagaa gaaaaattgg aaaaatagaa aggtaaaaac
     8101 aacattatgg aagataaaat tataataaga ggagcaaaag aacataattt aaaaaatata
     8161 gatttagaat taccaagaga taaatttatt gtttttacag gtttgtcagg gtctgggaag
     8221 tcttctttgg catttgatac aatatatgca gaaggacaga gaagatatgt agaaagtcta
     8281 tctgcttatg caagacaatt tttaggtcag atggaaaagc caaatgtaga atatatagag
     8341 ggtttatcac cagctatctc aattgaccag aagactacct caaaaaaccc acgttctacg
     8401 gttggaacag taacagaaat atatgattat ttgagactat tatttgcaag agtaggagat
     8461 gtgtattgcc ctacatgtgg tgaaaaaata agtcagatga ctattcaaga aatagttgat
     8521 aaaatgctag attttccaga tagaacgaaa ttacaaattc tttcaccaat tgttagagga
     8581 caaaaaggta cacataaaaa agtctttgat aatatcaaaa aagaaggatt tgtaagagtt
     8641 agagttaatg gtgaaaacta tgaagttagt gatgatataa tcttaaataa aaatcaaaaa
     8701 cataatatag aagttgtagt ggacagaata gtaattaaag atggaataga atcaagacta
     8761 gcagattcaa ttgaaactgc agttaagcta tctgatggtc ttgtaatagc ccaaataata
     8821 gatggagaag aaattctttt ttcaactaaa tttgcatgcc cagaacatgg aattggcata
     8881 gaagaattat ctccaagaat gttttcattt aatgctccct ttggtgcttg tgatgtttgt
     8941 aatggtcttg gggaaagtaa agaagttgac ccagaattag tagtaccaaa caaagattta
     9001 tcaataaaac aaggtgcagt ggcagcatgg ggttcaacag gtgttaatga tgacacttat
     9061 tacagtaaaa tgataaaaag tttagcagag cattttaatg tatctttgac aacaccgttt
     9121 aaagatttac cagatgattt tgtggaggaa ttactttatg gtaaagataa tataattgta
     9181 gagtttactt atgagtcaaa atttggagga actagaaatt ataaatcata ctttgaaggt
     9241 gtaatagtaa acttagagag aagatataga gaaactaatt cagattacat gagagataag
     9301 attgaagaat acatggcaga gagaccatgt ccaaagtgta aagggatgag acttaaaaag
     9361 gaagttttat ctgttttagt tgatggaaaa aatataatgg aagttacaaa cttatctgta
     9421 aatgaattga ttgactttat ggaaaatata aatcttacag aaaaacagag atttatagct
     9481 catgagatta taaaagagat aaaaggaaga gctatgttct taagagatgt aggtcttgat
     9541 tatttaaatt tatcaagaaa agctggaact ttatcaggag gagaagcgca acgtataaga
     9601 ttagctactc aaataggttc tgcattggtt ggagttttat atgtacttga tgagccaagt
     9661 atagggcttc accaaagaga taatgatagg cttataacta ctcttagaca tctaacagat
     9721 ttaggaaata ccttaatcgt tgtagagcat gatgaagaca caatgagaga agctgattat
     9781 gttatagaca ttggtccagg tgcaggtata catggtggtg aaatagttgc acagggtact
     9841 ttagatgaaa taattgaaaa tgagaactca ataacgggtc aatatttaag tggtaaaaaa
     9901 caaatattgc ttcctgagac tactagagaa ggaaatggaa gttttataga aatagttaaa
     9961 gcatcagaga ataatctgaa aaatattgat gtaaagttcc cattaggtaa gtttacatgc
    10021 ataacaggtg tttcaggttc tggaaaaagt acattaataa acgatatact ttacaaaggt
    10081 gttgcaagta aagtgaatag acttaagcaa agagctggta agcataagga gataaaagga
    10141 attgaaagta tagataaagt tataaatata gaccaaagtc ctattggaag aacacctcgt
    10201 tcaaatcctg ctacttatac aggtgtattt gattttataa gagatatttt tgcatctact
    10261 aatgaagcta aagcaagagg ttataaaaaa ggtagattta gttttaatgt aaaaggtggt
    10321 agatgtgaag cttgtaaggg tgatggaata ataaaaatag aaatgcattt cttaccagat
    10381 gtatatgttc cttgtgaaat ttgtaaaggt gaaagataca atagagaaac attgcaagta
    10441 aaatacaaag ataaaactat atctgatata ttagatatga atgtggaaga agctgttgag
    10501 tttttcgaaa atataccaaa tataaaaaga aaattagaaa ctttaatgga tgtaggactt
    10561 tcatatatta aattaggaca gccttcaact caattatcag gaggagaagc acaacgtata
    10621 aaattagctg ctgaacttag taaaagacca acaggaaaga ctttgtatat attagatgaa
    10681 cctactactg gacttcatat ggcagatgta gacaagctaa tccaagtatt acaaagacta
    10741 gcagatacag gaaatacaat tatagtcata gaacataatt tagatgttat aaagacttgt
    10801 gattatgtaa tagacttagg accagaaggc ggagataagg gtggaactat agttgcaact
    10861 ggaacaccaa aagaagtatc taaagttgat ggttcttata caggtcaatt cttaaagaaa
    10921 tatttttctt aagttatata aattgtgaaa aattttagag cagatatacg ataaactgtt
    10981 ttgttataag taaattagtc aaatataagt tgtaaatagt aatacaaagt aaagtccata
    11041 ttactcttca tggttaaaat tgttttcaaa tcaattatag aagggaatat gggctttaac
    11101 ttgtctaaag cactggaaat tgtataactt tttaaaattc atattttagg gtataatttt
    11161 agtataaaat ttaatgtttt ggagggcgag atgtttgata tacaagagca tttaaaaaaa
    11221 ttaccttctg agcccggagt atatttaatg aaagacaaat acgaccatat aatatatgtg
    11281 ggaaaagcca tatctctaaa aaatagggta aggcagtatt ttcaatcttc aaaaaaccat
    11341 acatctaaag taaaatctat ggttaagaat atatataagt ttgagtatat tataacggat
    11401 tcagagttag aggcattaat acttgaatgt aatttaataa aaagatacag accaaagtat
    11461 aatgtagtgc ttagagatga taaaacttat ccatatataa aagtaactac gaatgaagat
    11521 tatccaagaa tcttaaaagt tagaagagtt cttaaagata aggcaaagta ttttgggccc
    11581 tataccaata taagtgctgt gaatgatacc ttggagctta taagtagtac gtatccaata
    11641 agaagctgta aaatagatat agataaagca ataaagaata agacaagacc ttgtttaaat
    11701 ctacacataa ataaatgttt aggaccttgt acaggaaatg tttctaaaga agagtatgga
    11761 aaaatgatag aagagattat aatgtgttta tctggtaaag aagaaaaatt gatggaactt
    11821 ttaaaggaaa aaatgaatga gagttctatg aactttagat ttgaagaggc tgctgtatat
    11881 agggataaga taaagagcct tgaagagatg atacagaaac aaaaaataga tgctactgta
    11941 agtgacttaa accaagatgt ggttgcaatg gcaagagctc ataatgaagc ttgtgtccag
    12001 gtatttttta taagaaatgg taagattgta ggtagggaac attttatatt agaaggggta
    12061 atggatagtc caagagcttc tatattaagt tcatttgtaa aacaatttta taatgaacaa
    12121 gagtatatac ctaaagaatt aattatagaa gatgagatag aagatagttc tatattagaa
    12181 gaatggctgt catctaaaaa ggggcaaaaa gtaacaataa gagttcctca aaagggagag
    12241 aagaaaagct tagttgaaat ggttaggaaa aatgctgtag aatatcttga aaaattttct
    12301 gatatgaata aaagaaaata tgaaaaaagt attggagcat tggaagaatt aaagcaaata
    12361 ttaaatctag aaaagttgcc aataagaata gaggcttatg atatatcaaa tatacaaggt
    12421 gtagattcga ttggttctat ggtagtgtat acgaatgcta aaaaagataa gaaggaatat
    12481 aggagatata aaataaagac agttattgga cctaatgatt atgattctat ggctgaaatt
    12541 gtagatagaa gattaaaaca tggaaattta cctgacttga tattgttgga tggtggaaaa
    12601 ggacaagtta gtgcagtaaa aaaagtacta gaactcaatg atgtagatat acctctttgg
    12661 ggaatgtaca aagatgataa gcatagaact aaaggattga tatgcaaaga aaaagaaata
    12721 gaactagata aaacaactaa tttatataga tttattgcta gtatacaaga agaagttcat
    12781 aattatgcaa taacatatca tagaagtcta agaaataaag ctttgacaaa atctatctta
    12841 gatgatatac aaggaatagg tgaaaaaaga aagaaatcat tattaaatca ctttaaagat
    12901 gtagatgcta taaaaaaagc tactatggaa gaacttttag aagtagatgg aatgaataaa
    12961 agcattgcag ataatgttta taattttttt agaaaggaag aaaattaatg gaaactagca
    13021 ataaagtatc aataagagag ataataaaag agttgaagtt aaaagtagta tacatgcctg
    13081 atgatgtaga ttattatgtc tactctcaag atataaatag accaggatta cagtttgcag
    13141 gatattttga ttattttgca tatgaaagaa tacaaatagt tggaaagaca gaatataatt
    13201 tttttggatt aatggatagt catattcggg aagaagtact agatagattt ttctcatatg
    13261 aaataccagc gttaataatt tctagagact tggaaatcaa gccagatgta attgaaaagg
    13321 ctaaaaaata taaaagaata cttttgagca caaatagaaa taccactaga cttataaata
    13381 gactgtcaaa ctatttggat aataagttag caccacacac aacaattcac ggggtgctag
    13441 tagatattta tggtataggt gtactgataa aaggtgaaag tagtatagga aagtcggaaa
    13501 cagcattaga attgattcaa aggggtaata gattagttgc agatgatgct gtagaaataa
    13561 gaaaattaga tgaaagtatg cttgtgggtc agtcacctga actaataaga cattttcttg
    13621 agataagagg aataggaata atagatgtta gaagtttata tggagtaggg gcaatcaaaa
    13681 attctaaaat gatagattta gtagttcatt tagaagcttg ggatgaaact aagtattatg
    13741 atagactggg actggataaa gaatatgaag aaatacttgg tgttaatgta gagaaattag
    13801 ttgtacctgt taaacctgga agaaatactg ctatgatatt agaagtagca gcaatgaatt
    13861 ttagacaaag aggaatggga tacgatgcag ctcaagaatt tactaaaaaa ttatcaaaat
    13921 taatcgatac taagtagtac tgaataatag tgaactacac atcatttaat taaatattga
    13981 ttaggattat gtcctacacc ttataaatta aaatcaagtt gtatatatat aatgtttatt
    14041 aataagatat attatataag gataatttaa agtacagtgt tagattataa taatactaga
    14101 ttaattttat atttgagttt cattatataa caagaaagtt ggaatttttt atgggaatag
    14161 aatattttag taaactttta gaaagtagta ctgaaaaaga attgctctta agtaaaaagt
    14221 caaataacat aagtacagca agattaatat cttttttgat agtgatagca gggtttgcta
    14281 ttggatttta caataaaaat atagtaggta tttttgttgg agttttatca ataataatat
    14341 ttatagcact cttagttatt catagtaaag taaaagaaga agaaacatat tttaaaagta
    14401 agagcgaagt attaaataag tatattaaga gatttggaga tggttggaag gaatttaaaa
    14461 tagatggtaa agaatactta aaagatgaaa actcgcaagc aaaagattta gacttatttg
    14521 gaagagcatc attgtatcaa tatatctgtg tagctaatac atcttatggg aaaaattttt
    14581 tagccaaata tttgtggaat gaaaatccag atgagaatgt aatcttagaa agacagagag
    14641 ctataaaaga acttttatca aaagatgatt tttctattca tattcaaaca ctcagtaata
    14701 taattggtaa agagcaaaaa aataattcag atagtagtat agaatcgttt atagagtatg
    14761 gagaaaataa gaatgtgtat ataccaaaat ggatgcatat ttttacttgg ggtttaccaa
    14821 ctgcaacaat cttatctttt atattttgta tgttaggatt tttacctgtt ttacctgttt
    14881 ttttactttt tataatccag ttagggttta gtggttttgg atatccaaga cttatgcaaa
    14941 ctcttgctcc attattttca tttagtagaa gtatacaagt atatgaaaaa atgtttgagg
    15001 ttttagaaaa agagacgttt gagagtgcat atttgaaaga attacaagat aaactttcta
    15061 agggaagtgg agtttcaaga ggtgttaaac aattaaattc aataggaaat gctgtaaacc
    15121 taaggtataa tcagatacta tatataattg cctgtggtgt tttgatgtgg aactatcatt
    15181 gtgcagaagc tttggagcga tggaaaggta tatatggaaa tcaaattaga gattggtttg
    15241 aatctattgg agaatttgaa gctctaataa gtttaacagt tgtttctcat gtaaaggaaa
    15301 atacatgttt tccagtaata aaatatgaag atactccaag attgaaagtt gaagaggtat
    15361 accacccatt aatagcagaa aaaagtgtca tagcaaattc gatacaatta aattctcaga
    15421 cttgtattat cacaggttca aatatgtctg gaaagacaac atttttaaga agtatcggtg
    15481 taaacttagt gctggcatat gcaggtgctc cagtatgcgc taagagtttt gatgcgacct
    15541 gtatggctat atttacttct atgagaatac aagatgatgt aagtcaagga atatcaactt
    15601 tctatgctga gattcttaga ataaaatcaa tgattcaata tagtttaaaa gaactgccaa
    15661 tgttggttct agtagatgaa atttttaagg gaactaactc tgctgataga attattggag
    15721 caagtgaggc ggtaaagaaa ttatcaaaac cctgggttat aagtatggtg acaacacatg
    15781 actttgagct atgtgacctt tcaggtagtg gagatgttga agttgtaaac tatcatttct
    15841 cagagtacta tgtagacgat aaaattcatt ttgactataa gatgaaagat ggaagatgta
    15901 aaactacaaa tgcaaaacaa cttatgagaa tggcaggtat tctttaatta atagataggt
    15961 taattaaaga ataagtctat ttagaaatat aatttaattt gtgaaattaa taaggattgt
    16021 cttaaattac gtaaaagtat tttaagacaa tcctttttta agaataaatt ttatactttg
    16081 ttatgactac ttagcttctt cacaagcaca ttcttgtgga cactctccat agacacaact
    16141 cttatcaaag taatgagtat gaaggtattt atgagctaca tcactatttg gttctccaaa
    16201 gaagtcttta taaatagctt gtaaatcagg attctcatga gatttacgaa tagccttatt
    16261 tctatctatt tcatataacg catttgcacg tctatcaact atttcagtat taccatggtg
    16321 ataaggttga ccagcacctc caacacatcc accaggacat gccataactt ctatgatatg
    16381 gtatttacat tctccacttc tgaccttatc cataatcttt ctagcgtttt taagtgtatt
    16441 tacaatacac acattaactg tagttccatc aacatctata cttgcttccc taattccttt
    16501 aagacctcta acattagtga agtttacatt atctaaagtc ttattagtta tcttctcata
    16561 tgaagtacgt agggcagctt ctaaaactcc accacttgct ccaaatatag atgcagcacc
    16621 tgtagatttt cctagaggat tatcaaaatc ttggtcttga agattaggta aatcgatagc
    16681 agcttctttt atcatcttag ctaactctct agtagttata gaaaggtcaa catctagtat
    16741 tccagattca cttaattctt ctcttgaagc ttcatatttt ttagctacac aaggcattac
    16801 agatacaaca taaagttctt caggattgat tcctaatatt ttaggtgcat agtagttttt
    16861 agctatagaa ccaaacatac cttgaggaga cttacagcta gatgctaagt ttaacttgtc
    16921 tggatagttg tgctctaaga agtttaccca tgcaggacaa caacttgtta atataggaag
    16981 attttcacct ttttggattc tttctataaa ttcagtagct tcttccatta tagtaaaatc
    17041 tgcaccaaag tttgtatcaa atacatgttc aaatccaagt gcttttaaag cagctaccat
    17101 ttttcctgta gagattgacc ctggttctaa tccaaactct tcaccaatag caactcttac
    17161 ggcaggagca acttgcacta ctatagcctt ttctttttta tttaaaacat cccaaagttt
    17221 aggtacatta tctacttctg ttaatgctcc agttggacag acagatatac attgaccaca
    17281 gaatgtacac tctgtttcaa ccatatcaga gttaaagaat gtactaacta aagtattaaa
    17341 tccacgatta actccagata atgctcctac tgtttgtatg tcattacata cagtctcaca
    17401 tcttctacat aatatacact tagaatggtc tttaactaaa gatttagtag aagtatcttt
    17461 acctgcaaaa gatttagaac cttgatatct tatttttctt actcctaaat cagcagctat
    17521 agtctgtaat tcacaatctc catttttttc acagacaaaa caatcttgtg gatggtctga
    17581 taacaataat tcaacaatag ttctacgagc atttaatgct ttagcactat tagtctgaac
    17641 tttcatacct tcctttataa ctgttccaca agctggaact aatcctctct ctgtctcaac
    17701 catacaaaca cgacaagaag cacaagtgtc aagcttatct atttcattca tatgtaaatg
    17761 acaaaggttg tgtattttta tatttattaa tttcgcagca tctaagatag aagttccact
    17821 aggtgctgaa acgtgtttgc cattaatagt taaattaact aaactcattg ttattcctcc
    17881 tccaaataag ttatttcttg attatagcgt taaatgtaca attatctata caagcaccac
    17941 attttagaca cttagaagta tctattgtat gttgttcctt aacagaacct gttatacaac
    18001 cagcaggaca cactctagaa cattttgtac aacccttaca gtcatctgtt ataaagtaag
    18061 aaagtaagtt ctgacattta ccagcaggac atttcttatc tacaacatga gctatatact
    18121 catctttgaa atattgaaga gtacttaaaa cagggtttgg agcagcttta ccaagaccac
    18181 ataatgaagc agtttgtata gtttcagcaa ggttttctag gtcatctaaa tcttgtagag
    18241 taccattacc ttctgtaatt ttagttaata tttctaataa tcttgtagta cctatacgac
    18301 aaggtgtaca tttaccacaa gattcttcaa cagtaaactc aaggaagaac ttggctatat
    18361 ctaccataca gtcagattca tcaagaacaa gcataccacc agagcccatc attgaaccga
    18421 tagaacttaa tgaaccaaag tctataggag tgtctaaatg ttcagttggt atacatccac
    18481 cagatggtcc acctgtttga acagccttaa atttcttacc ttcagggatt cctccaccaa
    18541 tttcaaatac aatatctctt aaagtagtac ccattggaac ttcaacaagt cctacatttt
    18601 ctacttttcc accaagagca aagactttag taccactaga atcttcagta cctaagttgg
    18661 caaaccaatc tccaccctta agtataattg caggtatgtt agcaaaagtc tcaacgttat
    18721 ttaagcaagt aggagatttc catagaccac tcttagaaga actataagtt ttcattctag
    18781 gttcaccacg tctaccttca attgaatgca ttagcgctgt accttcacca caaacgaaag
    18841 cacctgcacc atattttaat tctaatttaa agttaaatcc agtgcctaag atattttcgc
    18901 caagtaaacc gtatttttca gcttgagcta tggctacttt taatctttct atagattttg
    18961 gatattctgc tcttatgtat atgtatcctg tatcagaacc tatagcgtat ccacatattg
    19021 ccatagcttc tagtacacta tgagggtcac cttcaagtac agatctatcc ataaatgcac
    19081 ctgggtcacc ttcatcagca ttacatacaa caaacttttt atctgttgga ccagctggat
    19141 tttttgaagc agcttcccat ttagagccag tagggaaacc tgcaccacct ctaccacgaa
    19201 ggccagaagt ttttacctct gcaataactt cttgaggagt aagagaagtt aaacatttgc
    19261 ctagagctaa ataaccatca ttggcaatat attcttctat actatctggg tctataagac
    19321 cacaattttt taaagctata cgtaactgtt ttttgtagaa agacatttct tcttgttctt
    19381 caactttttt acttaaagat tcttcctcat ataataattc ctcgacaact gtatttctaa
    19441 ttaagtggct ttgaactatc ttttcagcat cagatggctc aacttttacg tagaaaacgt
    19501 tatcaggata aacttttaca ataggacctt gagcacagaa accaaaacaa cctgtcaaac
    19561 gaacactaac tttatcttgg attcctgatt tctttatttc attttctaat tcactaacta
    19621 tttctagact gtttgaagat gtacatccag tatcacaaca aacaagtaat tctcttctta
    19681 atacattttc cttagaataa ttttttctta agctaagatt aggtttcagc tcatcagcta
    19741 aaacttttaa ttcatcaaat gaatttactt tacgcattat ttattcaccc ctcttaacaa
    19801 ttcaattcta attctctata agtatctaat atttcgctta cttggtcagg tttcacttta
    19861 ccataaactt taccatttac agtaacaact ggagctaaac cacatgcccc caaacatctt
    19921 aaaccttcta aagaaaattt aaaatcacta gtagtttctc caacttttat gccaagctgt
    19981 ttttcaaaag cacttaagat tttgtcagca ccttttacaa aacatacagt tcctagacag
    20041 acactgattt tatacttacc tacaggttct gtagtaaaat aagaatagaa tgtaacaacc
    20101 ccatatacct ttgcaggagc tactccaagt tttctagcaa catgaagttg aacttctttt
    20161 ggaagatatc caaatatacc ttgggcttca tgtagaactt gtattaacgc accttccttt
    20221 gttgcaagag agtcaataaa tatatctagt tcgtcaaata attgcttgtt gtgtgaaata
    20281 aaatcgcaca taacaaatcc cccttttctt tttaaagtta ttatttttca taaaataatt
    20341 aaccatgagt tgaccccaac attatgtggg tgaacaaaac caaaacaata aagaaagaat
    20401 aatatacaac aatttcttta tttataaaaa caatgcaaaa aagttaccta ttaaaaataa
    20461 aaactatact agtctattat aatataaaat aattcagaat agttaaaaaa aataaaaaaa
    20521 atgagctaaa ttcaataaaa aaaaaataat acatataaaa aactttaaaa aataaaccaa
    20581 aatgtaaaat ttttataaaa taaaagattt ataaaactgc tgataatagt cttgaaattg
    20641 attccttgat ttttatataa gtagacctag aattatatat atctaatgtt atttccttag
    20701 aatttaaaat atcattttca aatataattc tttgttccaa tgcttttttt gaagagtaca
    20761 taaatgcatt aacttcgaaa ttaagctcaa aacttctgat atccatatta gctgttccta
    20821 tagagcatat agagtcatct ataactattg tcttagcatg taaaaatgca ttttgaccat
    20881 aagtatagat tttagctcca aactttaaaa gttctccagc atatgaatat gatgcccagt
    20941 atacaaaggg atggtcaggt ttagaaggaa tcataactct cacatcaaca ccagataaac
    21001 aagctatttt taaagtatct ataaaagttg agtcaagtat taaataagga ctctgtatgt
    21061 atatatactt tctagctttc tgaatcattt taaggtaacc atatttaatc tcatcaagtt
    21121 ctgatatatc tggaccactt gatactatct gaatcccaat attttcagaa ggatgattag
    21181 aatttgtatc ttcttctata aagtactttt ctaaatctaa gtcttcttta gttgtatatc
    21241 tccaatcaag gatgaatctc atatttaggt caattacgca atcaccagta agcttagtat
    21301 gtgtatctct ccatagacca aattttggat ttctacctaa atattcatca ccaacattat
    21361 atccacctac aaatcctacc ttaccatcaa tgacaacaat ttttcggtga tttctatagt
    21421 ttaagttaaa gtttacaatt ttcaagaatg atgggaagaa gcttccaact ttaacaccac
    21481 tttctttaag tctggaaagt gtactatttt ttagtgttct accaccaact gcatcaaata
    21541 aaagtcttac ttcaacacct tctttagctt tgtctataag aatgtctatt atttcagttc
    21601 cgattttgtc atctttgaaa atgtaaaatt gtatgtttat atatttttta gcttttttaa
    21661 gttcctcaag aagactgtta aaaaattgac tagattcagc ataaatccac acatcgttat
    21721 tattggtata atgagcatta tttgaattag ccaatgcata aatcatatcc ttatgttgat
    21781 gtatatctga atcaatttca gatgtagatt ggagtttaac ctgagtatct agtatattac
    21841 ttttgattat cttatcatcc ttttctttca atctaaacat attgtttttt gaaatatttc
    21901 tgccaaaagc tatataaagt atgaatccaa gagcaggaat caatactaat ataagaagcc
    21961 atgttaaagt tgtatttatg tttctttttt ctctaaagat aaggtttagt ataaccatga
    22021 aattaattat atatattgta gtaactgcta tttcatatat tgacatttcg aaaaatgata
    22081 tatcaaacat aaaattccct ccttcaaata tttattaaaa taagtaatta tatata
//
LOCUS       DBGJFF010000060        21919 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.061, whole genome shotgun sequence.
ACCESSION   DBGJFF010000060 DBGJFF010000000
VERSION     DBGJFF010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21919
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.061"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(156..860)
                     /locus_tag="ACOIAF_002238"
     CDS             complement(156..860)
                     /locus_tag="ACOIAF_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HGZ2669247.1"
                     /translation="MNIKNKKHILKKFIAMVLIAGVVTVEAGAITASAAEPTNSPMSA
                     TVDQCDFLNVRSGASANDAVVGKINTGDKVEVLELHSNGWIKIKSVDNVTGWVNGDYL
                     TIQGGNVDAKVQNVLNLAFKQQGKPYKWGATGPNSFDCSGFTSYVYKNGAGVNLPRVS
                     RSQATVGKKVSRAELKPGDLVFFGSGGSINHVGLYVGDSKFIHSPQTGDVVKVTSMAP
                     GTNYAKRLITATRVLQ"
     gene            complement(1062..2975)
                     /locus_tag="ACOIAF_002239"
     CDS             complement(1062..2975)
                     /locus_tag="ACOIAF_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HGZ2669248.1"
                     /translation="MRESIDLEKESFFNRLFKKYKFRHLTLLILDICSVLVAFRMSLS
                     LTGNLNAFTTNDIIISVCIYIVLHIVSFRLFKCYNTLWRYAGEEEIISIFVATLAYLI
                     PIYIINKLLGFDYPIMFYVLNTIFIIMFTSGARIAYRAIRIVMNKTYSRGKVSNILII
                     GAGDAGEMVIQELKRNPELKKVAVAIIDDDKNKIGRIIHNVKIVGTTSDIKDVVEKYN
                     VDEIIFSIANIEKRRKKDIIDICKNTNCKIKTIPGIYEIIDGKVDIKQIREVEIEDLL
                     GREPIKTNLREISNYIEGKVILITGGGGSIGSELCRQIAGFNPKELIIVDNYENNAYS
                     IQQELLRKYKNKLDLKTVIASIREEKRMDEIFNKYKPEVVFHAAAHKHVPLMESSPGE
                     AIKNNIFGTLNIAGLSSKYRAKKFVLISTDKAVNPTNIMGATKRAAEMIIQTMNAESQ
                     TEFVAVRFGNVLGSNGSVIPLFKKQIEDGGPVTVTHPDIIRYFMTIPEAVGLVIQAGA
                     MAKGGEIFVLDMGEPVRILDLAKNLIKFSGFEPDVDIKIEFSGLRPGEKLYEELLMSE
                     EGLLDTEHKKIFIGRPIDVDREKITKYLKLLREITNNEEVEKIDGIMRELVPTYIKPE
                     DANIKEIATTREK"
     gene            complement(3059..4150)
                     /locus_tag="ACOIAF_002240"
     CDS             complement(3059..4150)
                     /locus_tag="ACOIAF_002240"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HGZ2669249.1"
                     /translation="MKTVCYLADGSNPHTLKWCSFFKNKGYDIHVISLNGGHMEGIKI
                     YNFGSNVEELKNENISKKTGYLGSIKQIKKLVNEIKPDILHAQYASSYGFIGSLLGYH
                     PYIISVWGTDIYDFPNNGFIQKQIIKHNFRKADYIFSNSRDMAKEANKYTAKHVDVTF
                     FGVDMDRFKPMEVEKEDAFVIGIIKSLEKKYGIEYLIQAFKMLKDEYKDKKIILKIGG
                     SGSQMDNLINLAKELGIENDVQFLGRISPENVSKTFNSFDVTVFPSLREGFGVAAIES
                     EACEVPVIVTNVGGHPESVWENETGLIVEPKQPEEIKNAIIKLMENDELRLNMGKKGR
                     QFVRENYEVNLNFNDIEKIYDSIFDKYKK"
     gene            complement(4156..5523)
                     /locus_tag="ACOIAF_002241"
     CDS             complement(4156..5523)
                     /locus_tag="ACOIAF_002241"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HGZ2669250.1"
                     /translation="MYFVSMFLMPLKKEYKFYILGGKKMKIAIAGTGYVGLVTGVCLA
                     EVGNENVVCVDVDEQKVKSMKLGIAPIYEEGLEELMVKNYNRGRIDYTTDYKKAYEDV
                     DVILIAVGTPERSDGSANLDYIKAVAKQIAETVTKDTLVVIKSTVPIGTNEEIEDYIN
                     RNLKNDVKVELASNPEFLSQGTAVKDTLHASRIVIGVESEWAREILEEIYKPFNQPII
                     VTNRNSAEMIKYASNNFLALKISFMNDIANLCEIAGANIEDVALGMSYDDRIGSKFLK
                     AGIGYGGSCFPKDTKALHWLSEHNGYELKTVKAAIEVNESQKLKLVRKASKLVESFQD
                     MKVAVLGLTFKPDTDDIREAPSIPNIKYLLERGARVTVYDPIGVNNTKKIFGDSILYA
                     DSVDDAIEDAEICFIMTEWKDVLNYDIHKYKEKMKNPLVLDGRNCYKIKDMEDLGIEY
                     YSIGR"
     gene            complement(5477..6229)
                     /locus_tag="ACOIAF_002242"
     CDS             complement(5477..6229)
                     /locus_tag="ACOIAF_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HGZ2669251.1"
                     /translation="MNEPLVSIITPVYNSEEFLSETIKSIQNQTYKNWQLLLVDDCSK
                     DNSSEIIKSFRKEDARIKYIKLEKNSGAAVSRNVGIKNAEGRFIAFVDSDDLWDSRKL
                     EIQIEYMLKENVGFSFTSYRYMRQDGSKTNKVARAPKKIDYEGLLRNTIIGCSTVVID
                     KEIVGEFSMPLVRRGQDTATWLQLLKKEKYAYGIQEDLVNYRLVGNSISSNKIKALKR
                     TWNTYRNVENLSLPKSLYVFCFYVFNAIKKRV"
     gene            complement(6277..7185)
                     /locus_tag="ACOIAF_002243"
     CDS             complement(6277..7185)
                     /locus_tag="ACOIAF_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HGZ2669252.1"
                     /translation="MYKYKFTVFTPTYNRAHLLENLYNDLKAQTYDFNDFEWLIVDDG
                     SSDSTKELVEKFISENKLNIRYIYKENGGKHTAINLGVKNADGELFFIVDSDDGLIKD
                     SLEIANDEWDSLENKEGFSGVVGLCVYPSGDLIGTEMPEDKKICHFADLYFKYGVKGD
                     KSIVFVTDELIKFPFPERKEVRFLPESVVWNEMSKYYKVICVNKPMIIRDYLDDGLTK
                     NILSKNALRGRALEFLYLINQNTYPLSRYPYMWIKNYINLARYSLLSDSHYFGELRKV
                     SDKLLYLALFPLGYYKYIGQRKLVSK"
     gene            complement(7207..7956)
                     /locus_tag="ACOIAF_002244"
     CDS             complement(7207..7956)
                     /locus_tag="ACOIAF_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HGZ2669253.1"
                     /translation="MKKNLVSIITPMYNSEKFIEATIKSVLNQTYQEWEMLIIDDCST
                     DNSPNIVKSYMQQDSRIKCIKTETNKGVSNARNLALSKATGQFIAFLDSDDQWNSSKL
                     EKQVNFMLENDYVISFTSYELMDENDKKLNKVIKVPPNVDYRRLLKGNILGCLTVVID
                     KSKLDFEIRMSGVRHEDYVLWLSILKKGHIAHGINEVLALYRKSSNSLSGNKIKAAMW
                     TWNIYRNIEKIPLYKAIYYFINYGINGIKKS"
     gene            complement(7985..9160)
                     /locus_tag="ACOIAF_002245"
     CDS             complement(7985..9160)
                     /locus_tag="ACOIAF_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HGZ2669254.1"
                     /translation="MDKVKFLINMLLAFFIYPFNKHKFNGRDIWLIGGHSGDIYNDNS
                     KFFYEYMLKEHNDVETYWVVNKDSKVFDKIPGKKLIRGSVENYLYYYNSKAIVFSHAP
                     SADIAPYNFAVPVLNYFHKKTIKVFLNHGTISFKKRKPMNKKFKNIIDNLYKSYNIVT
                     ASSEFERNVMVNDWGMLDDSVYIIGNARYDNLPTNEVAQTRDILYTPTWRDWIKFSSG
                     KFTDTDYFKNIMNFLNDDKLNKILDEKDINVKIYMHHLMHEFIDDIKENITGKRIVFL
                     DKGVTLANEIRKSAANITDYSSVAIDFLYMNRPILFYQFDLDEYMEKVDSYIDLKSEM
                     FGSLAYNNDEAVNKLIDIIENNFEVMDNQKNERNKFFRYNDNKNCKRIYDCVLSKIK"
     gene            complement(9175..10014)
                     /locus_tag="ACOIAF_002246"
     CDS             complement(9175..10014)
                     /locus_tag="ACOIAF_002246"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HGZ2669255.1"
                     /translation="MLISLIMPTLNRYDDIYLLMDSLENQTYKNFELIVVDQNDNSKV
                     KEIVDKYIDKLDIKYIKSSKKGLSYNRNVGIDNAVGQIIGFPDDDCVYENDTLEKVIN
                     FFNKNKDYKIYSCKTMDSNKVDAFKKMYDGTCDITSSNVLDTITSITFFIDFEGKDYT
                     RFDEKLGVGGEFGAGEEIDYVLNLLSLGFKGKYFGNDIIYHPAKKHSKSKEKYQKDYN
                     YGRGFGALCKKEIVYRKNYKFAKVMVSKLVRNIGGLILSSNRDYHSATIKGRINGFRQ
                     YKL"
     gene            complement(10017..11174)
                     /locus_tag="ACOIAF_002247"
     CDS             complement(10017..11174)
                     /locus_tag="ACOIAF_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide polymerase"
                     /protein_id="HGZ2669256.1"
                     /translation="MKTLNDDRNIMIRKYLYIFVLFIILLRKYMISFIDPNMDIGMIK
                     SALFYSSIGILMLLFLFDKRKSITEMVLVGVCVLLYLLNREGAILLIVLLAVSAKQID
                     DKFIVKNYLIISACFLMVAILLFNLFPSLIFNQEVHYRYIEKIDMLVTRMDFGLGNPN
                     SVFYHMVTIYAAYIFLRYKDYNKWDRIILFGSAFFVYQTTYSRTGFFTILAGLIFVEI
                     IRWIDIKKIKGLPMLLKTLPIILTLFSVIIGTVFDKSTLLNRLLASRPKFWHVYLAEK
                     GNFLKPFGNSYSPAIKATNPLDSSYVYIISVLGVVACILFMYLMYKGIESFIEKDKKA
                     YLVALFIFLLYSFAENLLLEASFSFAVVLLIKEVILNDKREIDLWKMKSRR"
     gene            complement(11190..11912)
                     /locus_tag="ACOIAF_002248"
     CDS             complement(11190..11912)
                     /locus_tag="ACOIAF_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HGZ2669257.1"
                     /translation="MIPKKIHYVWFGGPKGNIENICINSWKEKLPEYEIVEWNEKNFD
                     IEKEIKGNKFLEECYKRKLWAFISDYTRIKVLYEQGGVYMDTDMQILKDITPLLENNR
                     LICGYEDDREYINGAIIGVEKGHPFLKDLLEYYEKEVLTSSLFTIPKIMTHLMEKNYK
                     KIDPNNYEEGIRVYDKEYFYPFGFKEDFTPECITENTFGIHWWGKSWAKKRNYFLESK
                     HLTGVNKIWKCCKIFASNTLRS"
     gene            complement(11924..12997)
                     /locus_tag="ACOIAF_002249"
     CDS             complement(11924..12997)
                     /locus_tag="ACOIAF_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HGZ2669258.1"
                     /translation="MKVYNVIMAGGGGTRFWPLSRQEVPKQLINLSGEDALINETINR
                     IDSLAKKDDLFIVTNEKQLEALKDIVKDKCLDSNILPEPCARNTAAAIGFAAFNIMKK
                     YGDGVMCVYPADHYIKDEKEFKSILEKAIYIAENNYKLVTIGITPTFPSTGYGYINFN
                     RENTIEDVAYEVVEFVEKPNYEIAKEYVNSKKYVWNSGMFVWKVSKILEDFKRYLPKV
                     YEKLEDISKYLGTKEEMEKIKEIYPTIQSISIDYGIMERSNDVIVVPGDFGWNDVGSW
                     DSLGAIYPTDDEGNIKRGENITIDTKNSIIYSDDKLISTIGISDLIVVSTNDAVMVCR
                     KDKAQDVKKIVEQLKEEDRQEYM"
     gene            complement(13019..14725)
                     /locus_tag="ACOIAF_002250"
     CDS             complement(13019..14725)
                     /locus_tag="ACOIAF_002250"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HGZ2669259.1"
                     /translation="MDYKNNYEMWLNSPYFDEQTKNELLSIKDDEKEIQDRFYKNLEF
                     GTGGLRGIIGAGTNRINIYTVRRATLGVLNYIMKTQGEEGKQKGIVIAHDSRYMSREF
                     CIEVAKTLSAYGVKAYIFEELKPTPELSFAVRYLKCAMGIVITASHNPKEYNGYKVYD
                     SDGGQICIDMANDIIAEVNKIDDYSTIKSIDFEEALSKNLITILDNEVDDEFIKAVKK
                     QVLRQNIIDEYGKKLKIIYTPIHGTGNKPVRKVLNECGFENVMVVKEQELPDSNFSTV
                     KYPNPEEKSVFNIAIEMAKNNGTDLIIGTDPDCDRVGIVVKDSSGEYVVLNGNQVGSL
                     LVRYILESLVEENKLPKNNPTIIKTIVTSELGAKIAKAYNVDCLNTLTGFKFIGEKIK
                     AFEESNDRSFIMGYEESYGYLIGTHARDKDGVVSSLMICEMAAYYSSKGMNLYEALID
                     TYNKFGYYKEDLKSVTLKGIDGIKKIKEMMLYFRSVKIDNVADVKVDKILDYKDGVDD
                     LPKSDVLKFLLEDGSWIAIRPSGTEPKIKFYFGANSDNQEDVEFKLNNLISYILNVVD
                     SI"
     gene            complement(14771..16327)
                     /gene="murJ"
                     /locus_tag="ACOIAF_002251"
     CDS             complement(14771..16327)
                     /gene="murJ"
                     /locus_tag="ACOIAF_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HGZ2669260.1"
                     /translation="MSKTAKAALWIMAATMFSKVLGFLRELVLANFYGTGMYADVFVL
                     TLNIPGLIIAVIGSAVATTYIPMYFETKKRLGDEGALKFTNNVLNICYIMAIVIAIIG
                     LLFTEQFVTVFAAGFRNDPAKFQAAILFTKIMISGVLFLSGSKIFSSFLQVNDSFVIP
                     GLIGIPYNIIIIAAIALSAGKNVWILPAGALLAMASQLLFQLPFAFKKSYKYKPYINL
                     KDESIKELVNLVLPMLVGVAVGQLNIFVDRLLATTLGDGKLSALNYANRLNEFVMALF
                     VTSIITVIYPKLAKMSGKDNKEGFISTIVKSSNCIILVVLPISIGAIILAEPLVRILF
                     QRGKFDALSTDLTSIALRLYSLGLLACGVRDVLYRAFYSLSDTKTPMINGSIALIINI
                     VLNLILIRPLGHAGIAISTSTSNIITVILLFISLKKKNGYFGGDKIIKTGLKSLVASG
                     VMAVATLLIYNNLYAFMGSGTIKEIISVGAGVLGGASVYTVLIVLFKVEEMDLAFEFL
                     RKGKQKLLRR"
     gene            complement(16442..17497)
                     /locus_tag="ACOIAF_002252"
     CDS             complement(16442..17497)
                     /locus_tag="ACOIAF_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp7"
                     /protein_id="HGZ2669261.1"
                     /translation="MIKKISTILSLVLLISISSTIGVFADANPKRELIEGSIPEISTE
                     LNKRAFKDSKEVILVNEESIVDSISATPLAYAKNAPIVVTKSKNLGRVTRNYLKELGP
                     EKVTIVGGLKAVSKDAERNIEKMGMKVERIRGKDRYDTSLKIAREMYRTVGFDEAFLL
                     SSTTGLENAISVYSYAAKSGMPIIWAKDEGFEEQIDFLKGKNLKKIYALGDSKEFIAE
                     IDSNLKNIEGIKQINKSSTNVDLIKKFYDEKDIKKIYTARLDFGSRSDVNEYISLGVV
                     SAKENMPILICSDNLSRAQDKFLKDSNINDVVEVGYTVGDYSLFKSIFNLTFLSCIVL
                     ILLLLLITFRALRYESK"
     gene            complement(17592..18278)
                     /locus_tag="ACOIAF_002253"
     CDS             complement(17592..18278)
                     /locus_tag="ACOIAF_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HGZ2669262.1"
                     /translation="MSGYTNDECEIPKIKSYPGADKEIASEIDYSIVKGTVLFDLYQR
                     IMDLVLSIIGLVIGLPLIAIFGILIKIEDKGPITYKQERLGKCGRRFYIYKLRSMRTD
                     AEKFGAQWAEKDDPRITKVGKFIRKTRIDEIPQLFNILKGDMGLIGPRPERPNFTVQF
                     NEEIPGFINRLAIKPGLTGWAQVNGGYEITPEEKLKEDIYYIKNRSILLDFKILFKTV
                     KVVLTGDGAR"
     gene            complement(18498..20522)
                     /locus_tag="ACOIAF_002254"
     CDS             complement(18498..20522)
                     /locus_tag="ACOIAF_002254"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HGZ2669263.1"
                     /translation="MKKTTKLLATGMLSVAMVAPNVALAAENTTANTESNSDININLQ
                     RKSVVLGSKSNASVKFKEKLNADSITLNFMCYDMPLEATLNYNEKTDSYEGVINYNKD
                     PEYLNVWELQSIKINGKDEQKVLNKEDLESMGLNLKDYDVTQEFIISDANSTKAVNEY
                     MRKTSAPVKKLAGATRFETAVEISKQGWKDGSSKVVIVNGELAADGITATPLASTYDA
                     PILLANKDDIPESTKAELKRLNPSDVIIIGDDGSVSQKAVSQIKSAVNVNVTRIGGVD
                     RHETSLLIAKEIDKYHDVNKIYIANGYAGEYDALNISSKAGEDQQPIILANKDSVPQG
                     TYNWLSSQGLEEAYYIGGSQSLSSKIIDQISKIAKNGTSKNRVSGADRHETNANVIKT
                     FYPDKELSAMLVAKSDIIVDSITAGPLAAKLKAPILITPKTYVSAYHSTNLSEKTAET
                     VYQIGDGMKDSVINSIASSLSKHNAPTEPDNSGSAAGKTVVIDPGHGGSDSGATSGLN
                     GGAQEKKYTLNTALATTEYLRSKGINVVMTRDTDKTMALGERTALSNTIKPDLFTSIH
                     YNASNGAGNGVEIYYKVKDKNGGTTKTAASNILKRILEKFNMKNRGIKTRTLDNGKDY
                     LYVLRNNNYPAILVECAFIDNKSDMDKLNTAEKVKTMGTQIGIGIEDTVK"
     gene            complement(20776..>21919)
                     /locus_tag="ACOIAF_002255"
     CDS             complement(20776..>21919)
                     /locus_tag="ACOIAF_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HGZ2669264.1"
                     /translation="ISNNAVDSKLMNSRIKSIDIIRGLSIALMIVCNNPGTWMRMYPQ
                     LRHAVWHGVTLADFAFPFFVISLGVTIPISINSKLKNNKSTPSIILSIFKRSILLILF
                     GFFLNYLGNPDLDTVRILGVLQRMGLVYFVTSLVYLLLKKLNVGSTATIITFLCISTF
                     IIVGYYILAKPYGFELEGSLAQLVDLHFFKGHLYKPEFEPDGFLTSIVAISSGMLGCT
                     MGCVLLKEDIGEYKKFFKILVISIILLIGAFIFNQYFPFNKRLWSSSFVLLMAGSYGI
                     LLSIFYFICDIKNKSKIFTPIIALGSSPIFTYMCLEILSHVFWNVPKLTNKVDYPTTL
                     VEWTTYELITPWAGTTWDSLIFSLLYVLFWVIVMSIMYKKKIFIKI"
BASE COUNT         6835 a         4061 c         2291 g         8732 t
ORIGIN      
        1 taaaaaaaac agcaaagaaa tttatcttct ttgctgtttt tataacgttc atcactattt
       61 taatattact aaactcttaa atctctatgt aaaacattaa atgtaattat atatttaatg
      121 ttaatattca atcctatgta aatcaatatc tattattatt gtaaaacacg tgtagcagtt
      181 ataagtcttt ttgcataatt agttcctgga gccatgctag taacttttac tacatctcct
      241 gtttgtggtg aatgtatgaa tttagaatct cctacataca atccaacgtg atttattgaa
      301 ccacctgaac caaagaaaac taaatctcct ggttttaatt ctgctcttga tactttcttt
      361 cctactgttg cttgtgatct tgatactctt ggtaaattaa ctcctgctcc atttttatag
      421 acataagaag tgaacccaga acaatcaaaa gaatttggtc cagttgctcc ccatttatat
      481 ggcttacctt gttgcttaaa tgctaagttt aaaacatttt gtacctttgc atctacattt
      541 ccaccttgaa tagttaaata atctccattt acccatcctg taacattgtc aacacttttt
      601 attttaatcc atccatttga atgtaattct aaaacttcaa ctttgtctcc tgtgtttatc
      661 tttcctacta ctgcatcatt tgcactagct ccacttctta catttaaaaa atcacactgg
      721 tctactgttg cactcatagg tgaattagtt ggctctgctg cactagccgt tattgctcct
      781 gcttctactg tgacaactcc tgctattaac accatagcta taaatttttt caatatatgt
      841 tttttatttt taatattcat cataatctca aatacctaat cctttcgaaa atttaacatt
      901 ttgttccttc gtttccaatt cctaattaac attctatctt acatcaaaat aaattgcaac
      961 cttttttgta atctttttgt aatctttttg taatcttttg tttcttttta gtatatattt
     1021 gtttcttttt gctccccctg gttacaaatt agaaatatat tctacttttc tctagttgtt
     1081 gcaatttctt tgatattagc atcttctggt ttaatataag ttggcactaa ttccctcata
     1141 ataccatcta ttttttcaac ctcttcattg ttagtaattt ctcttaaaag ttttaagtat
     1201 tttgttattt tttctctatc aacatctatt ggtctaccaa taaaaatctt tttatgctct
     1261 gtatccaaca gaccttcttc tgacataagt agttcttcat ataacttttc tcctggtcta
     1321 agaccagaaa attcaatttt tatatcaaca tctggctcaa atccactgaa tttaattaag
     1381 ttttttgcta aatccaatat tcttacaggt tctcccatat caagtacaaa tatttctcca
     1441 cctttagcca tagccccagc ttgtatgact aagcctactg cttctggtat tgtcataaaa
     1501 tatctaataa tatctggatg agtaactgtc actggtccgc catcttcaat ttgcttctta
     1561 aaaagtggaa ttacactacc attactacca agcacatttc caaatctaac agcaacaaat
     1621 tcagtctggc tttcagcatt catagtctgt ataatcattt cagcagctct tttagtagct
     1681 cccattatgt tagtagggtt tacagcctta tctgtagata taaggacaaa tttcttagct
     1741 ctatattttg aagacaagcc agctatgttt aaagttccaa atatattatt ctttatagct
     1801 tcgccagggc tcgattccat aagaggaaca tgtttatgtg ctgctgcatg aaataccact
     1861 tctggtttat atttattaaa tatttcatcc attctttttt cttctcttat tgatgctatt
     1921 actgtcttca aatctaattt atttttatat tttctaagta attcttgttg tattgagtaa
     1981 gcattatttt cataattatc aactataata agctctttag gattaaatcc tgcaatctgt
     2041 ctgcaaagct ctgaaccaat tgagcctcct cctccagtta ttaggattac ttttccctca
     2101 atgtaattac ttatctctct taaatttgtt tttataggct ctctacccaa caagtcttct
     2161 atctctactt ctctaatctg ttttatatca acttttccat ctataatttc ataaattcct
     2221 ggtattgtct ttattttaca gttagtgttt ttacatatat caataatatc tttttttcta
     2281 cgtttctcta tgtttgctat tgaaaagatt atctcatcta cattgtactt ttctaccaca
     2341 tcttttatat ctgaagttgt tcctacaatt ttcacattat ggattattct tccaatttta
     2401 tttttatcat catcaattat agccactgct acttttttaa gttctggatt tctttttagt
     2461 tcctgtataa ccatttctcc tgcatctcct gcaccaatta taaggatatt tgaaaccttt
     2521 cctctagaat atgtcttgtt catgactatt cttatagctc tataggctat tcttgcgcca
     2581 cttgtaaaca ttataataaa tattgtattt aatacataaa acattatagg ataatcaaac
     2641 cctaataatt tatttattat ataaatcgga attaaataag ctaaagttgc cacaaaaata
     2701 gatataattt cttcttctcc tgcatatctc cataatgtgt tgtagcactt aaacaatcta
     2761 aatgatacta tatgaagtac tatatatata cacactgaaa ttattatatc attagtggta
     2821 aatgcattta aattacctgt caagcttaaa gacattcgaa acgctactag tacgctacaa
     2881 atatctagaa ttaatagtgt taaatgtcta aatttatatt ttttaaaaag cctattaaaa
     2941 aagctttctt tctctaaatc tatactttct cgcataacta gaagccctcc tttaaacagc
     3001 aaaagctacg cacctaagca tagccaacaa catctatctt ttgctgtatt ttttcatttt
     3061 attttttata tttatcaaat atagaatcat aaattttttc tatatcatta aagtttaaat
     3121 taacctcata attttctcta acaaattgac gccctttctt acccatatta agtctaagtt
     3181 catcattttc cattagtttt attatagcat tttttatttc ttctggttgt tttggttcta
     3241 caattaagcc agtttcattt tcccatacag attctggatg tcctcctaca tttgtaacta
     3301 taacaggtac ttcacatgct tctgattcta tagctgccac tccaaatccc tctcttaaag
     3361 aaggaaatac agttacatca aaagaattaa aagtcttgct tacattttct ggagatattc
     3421 ttcctaaaaa ctgaacatca ttttctatac ccaattcttt agctaaattt attaggttgt
     3481 ccatctgact tccagagcca cctattttaa gaattatttt cttatcttta tattcatctt
     3541 taagcatctt aaatgcttgt attaaatatt ctattccata ttttttttct aaactcttta
     3601 taattcctat tacaaaagcg tcttcttttt ctacttccat aggcttaaac ctatccatat
     3661 ctactccaaa aaaggttaca tctacatgtt tagctgtata tttattagcc tctttagcca
     3721 tatctctact atttgaaaag atatagtctg cttttctaaa gttatgtttt ataatttgtt
     3781 tttgtataaa accattatta gggaaatcat aaatatcagt accccaaaca gaaataatat
     3841 atggatgata cccaagtaga cttcctataa atccataact acttgcatac tgtgcatgca
     3901 atatatctgg ctttatttca tttactagct tctttatctg ctttattgaa cctaagtatc
     3961 ccgttttttt agaaatattt tcatttttta gttcttctac attagaacca aaattataaa
     4021 tttttattcc ttccatgtga cctccattca aagagattac atgaatgtca tatcctttat
     4081 ttttaaaaaa actacaccat tttaaagtat gaggatttga cccatcagct aaataacaaa
     4141 cagtcttcat aatatttacc ttcctatcga ataatactca attcctaaat cttccatatc
     4201 tttaatttta taacaatttc ttccatcaag gactaaagga tttttcatct tttccttata
     4261 cttatgtata tcataattta atacatcttt ccattctgtc attataaaac atatctctgc
     4321 atcttctatc gcatcatcta cagaatcagc ataaagtata gagtcaccaa atattttctt
     4381 agtattattt acaccaattg ggtcgtatac agtaactctt gcccctcttt cgagcaaata
     4441 ttttatattt ggtatagatg gtgcttctct tatatcatca gtatctggtt taaaagtcaa
     4501 acctaaaaca gcaaccttca tatcttgaaa gctctctact aacttagatg cctttctaac
     4561 taatttaagt ttttgacttt cattaacttc tattgcagct tttactgttt ttaattcata
     4621 accattatgt tcagataacc agtgaagagc ttttgtgtct tttggaaagc aagaaccacc
     4681 atatcctatt cccgccttta aaaacttact tcctattcta tcatcataac tcattcctaa
     4741 agctacatct tctatgttag ctcctgctat ttcacaaaga tttgcaatat cattcataaa
     4801 tgatatttta agagctaaga agttatttga tgcgtatttt atcatctcag cactatttct
     4861 atttgtaact attatcggtt gattaaaagg cttatagatt tcctctaata tttctctagc
     4921 ccattcactt tctacaccta taacgattct tgaagcatgt agagtatctt ttactgctgt
     4981 tccttgtgat aaaaactctg gattagatgc aagctctact tttacatcat tttttaggtt
     5041 tctatttata taatcttcta tctcttcatt tgtaccaatt ggaactgtag attttattac
     5101 tactagagta tcttttgtca cagtttcagc tatttgcttt gcaactgcct ttatataatc
     5161 taggttggct gagccatcac ttctctcagg tgtacctaca gctattaata ttacatctac
     5221 atcttcataa gcttttttat agtctgtagt gtaatctatt cttcctctgt tgtaattttt
     5281 aaccataagt tcttctaggc cttcttcata tataggtgct attccaagtt tcattgattt
     5341 aaccttttgt tcatctacat caacacagac tacattttca ttgcctacct ctgctagaca
     5401 aacacctgta accaaaccta catatcccgt cccagctata gctattttca tttttttacc
     5461 tccaagtata taaaacttat actctttttt taatggcatt aaaaacatag aaacaaaata
     5521 catatagact ttttggtaaa cttaaatttt caacatttct ataagtattc caagttcgtt
     5581 ttaatgcttt tatcttgtta cttgatatag aatttccaac taatctgtaa ttcactaaat
     5641 cttcttgaat cccatatgca tatttttcct ttttaagtaa ctgtaaccag gttgcagtat
     5701 cttgtcctct tcttaccaaa ggcatgctga actctcctac aatctcctta tcaatgacca
     5761 ctgtagaaca tcctattata gtatttctaa gtaatccttc atagtcaatt ttcttaggag
     5821 ctcttgcaac cttattcgtt tttgacccat cttgtctcat atatctataa gatgtaaaag
     5881 aaaaacctac attttctttt aacatatatt ctatctgaat ctctaatttt ctactatccc
     5941 ataaatcatc actatctaca aatgcaataa atcgaccttc cgcattctta ataccaacat
     6001 ttctagatac tgcagctcca gaattttttt caagctttat atattttatt ctagcatctt
     6061 cttttcgaaa actttttata atttcacttg aattgtcctt agaacaatca tccactaata
     6121 aaagctgcca atttttataa gtttgatttt ggattgactt tatcgtctct gataaaaact
     6181 cttctgaatt ataaacagga gtaattatcg atactaaagg ctcattcata tttttactct
     6241 ccttatatag caactagcag tactatacca acaagtttac ttagatacta attttctttg
     6301 tccaatatat ttgtaatatc ctaatgggaa taatgctaaa tacaacaatt tatcagatac
     6361 ttttcttaat tcaccaaaat aatgactatc agacaacaat gagtatctgg ctaaatttat
     6421 gtaatttttt atccacatat atggatatct acttaatgga tatgtatttt gatttattaa
     6481 atataaaaac tctaatgctc tacctcttaa tgcattttta cttaatatat tcttagtcaa
     6541 accatcatcc aaataatctc ttattatcat aggtttattt acgcatataa ctttatagta
     6601 tttagacatt tcattccata ctacactttc tggtaaaaat ctcacttctt ttctttctgg
     6661 aaaaggaaat tttataagct catcagttac aaatactatg cttttatctc ccttaacgcc
     6721 atacttaaaa tataggtctg caaagtgaca tattttctta tcttcaggca tttctgtccc
     6781 tatcaaatct ccacttggat atacacaaag tccaacaact ccactaaatc cttctttatt
     6841 ctctaaagaa tcccactcat cattagcaat ttcaagagaa tctttaatta aaccatcatc
     6901 actatctaca atgaaaaata gctctccatc tgcatttttt acacctagat ttattgcagt
     6961 atgttttcct ccattttctt tgtatatata tcttatattt aatttatttt ctgatataaa
     7021 tttttcaact aactctttag tactgtcgct acttccatca tcaacaatca accattcaaa
     7081 atcattgaaa tcataagttt gagcttttaa atcattgtac aaattttcaa gtaaatgagc
     7141 tctgttatac gtcggagtaa atacagtaaa tttatattta tacattaatt aaatcttcct
     7201 ctctgtctaa ctttttttga ttccgtttat tccataatta ataaagtagt atatagcttt
     7261 gtataaaggt attttttcta tatttctata tatattccaa gtccacatag cagcttttat
     7321 tttattacca cttagggaat tgctagattt tctatataat gctagaactt catttattcc
     7381 atgagcaata tgcccttttt tcaatatcga taaccataaa acataatctt catgtcttac
     7441 accactcatt cttatttcaa aatccaattt agacttatca atcacaacag taagacatcc
     7501 taagatattt ccttttaata atcttctata atctacatta ggtggaactt ttattacttt
     7561 gtttaatttt ttatcatttt catccatcag ttcatatgaa gtaaatgaaa ttacatagtc
     7621 attttctaac ataaaattta cttgtttttc taacttacta ctattccatt ggtcatcact
     7681 atctaaaaaa gctataaatt gtcctgttgc cttacttagt gctaaatttc tagcattaga
     7741 gacaccctta ttagtctcag tctttataca ttttattcta ctatcctgtt gcatataaga
     7801 tttgactata ttaggactat tatctgttga gcaatcatca ataattaaca tttcccattc
     7861 ttgataagtt tggtttaata ctgattttat ggttgcttca ataaattttt cagaattata
     7921 catgggagta attatagata ctaaattctt cttcatatta ccttcctcat catttataaa
     7981 atatttattt aattttactt aatacacaat catatatcct cttgcagttc ttattatcat
     8041 tatatctgaa aaacttattt ctttcatttt tttggttatc cataacctca aaattatttt
     8101 caataatatc aataagttta ttcacggctt catcattatt ataagccaaa gacccaaaca
     8161 tttcactttt taaatctata tatgaatcaa ctttttccat atattcatcc aaatcgaatt
     8221 ggtaaaacaa aataggtcta ttcatataca aaaagtctat ggctacactt gaataatcag
     8281 ttatatttgc agctgatttt ctgatttcat ttgctagagt taccccttta tctaaaaaca
     8341 caattcgttt tcctgttata ttttctttta tatcatctat aaactcatgc ataagatgat
     8401 gcatataaat ttttacattt atatctttct catctaatat tttatttaat ttgtcatcat
     8461 ttaaaaaatt cattatattt ttaaaataat ctgtatccgt aaattttcct gaactaaatt
     8521 ttatccaatc tctccatgta ggagtatata atatatctcg agtttgtgca acttcatttg
     8581 ttggtaaatt gtcatatctt gcatttccaa ttatataaac tgagtcatct aacattcccc
     8641 aatcatttac cataacattt ctttcaaatt cagaacttgc tgtaactata ttatagcttt
     8701 tatataggtt atctataata tttttgaact ttttgttcat aggttttctt tttttaaaac
     8761 ttatagtccc atgatttaaa aacactttta tcgttttttt atgaaaataa ttaagaacag
     8821 gtacagcaaa attgtatggt gctatatctg ctgatggtgc atgtgaaaat actatagctt
     8881 ttgaattgta ataatacaga tagttttcta cacttcctct tattaatttt ttgcctggaa
     8941 ttttatcaaa tactttactg tctttattta ctacccaata agtttctaca tcattatgtt
     9001 cttttagcat atactcataa aaaaactttg aattatcatt gtatatatcc cctgaatgtc
     9061 cacctataag ccagatatct ctaccattaa acttatgctt attaaatgga tatataaaaa
     9121 aagccaaaag catatttatt aaaaatttaa ccttgtccat aaacaccatc ctcattataa
     9181 cttgtactgt ctaaatccat taattcttcc ttttatagtg gcactatgat aatctctatt
     9241 ggaactcaat ataagacctc ctatatttct tacaagtttt gataccataa cttttgcaaa
     9301 cttataattt tttctataga ctatttcctt tttgcaaagc gctccaaagc ctctcccata
     9361 gttataatct ttttgatact tttctttaga tttagaatgt ttttttgcag gatggtaaat
     9421 tatatcattt ccaaagtatt ttcctttaaa acctaaactc aataagttta aaacataatc
     9481 tatctcttca ccagcaccaa actctccacc aacacccaat ttctcatcaa atcttgtata
     9541 gtctttacct tcaaaatcaa taaaaaaagt tatggatgtt atagtatcca aaacgttgga
     9601 acttgtgata tcacaagttc catcatacat tttcttaaat gcatcaactt tatttgaatc
     9661 catagttttg caactgtata ttttataatc tttattttta ttaaagaaat ttataacttt
     9721 ttctaatgta tcattttcgt agacacaatc atcatcaggg aaacctataa tttgccctac
     9781 agcattgtct atgcctacat ttctgttata actcaatcct tttttagagc tttttatata
     9841 ctttatatct aacttatcaa tgtatttatc aactatttct ttaactttgc tattatcatt
     9901 ttggtctact actataagtt caaagttttt gtaagtttga ttttctaagc tgtccataag
     9961 taagtatata tcatcatatc tatttaatgt tggcattatt aatgatatta acattactac
    10021 ctcctacttt tcatcttcca caaatctatt tcccttttat catttaatat cacttctttt
    10081 attagtaata ctactgcaaa actaaatgaa gcttctaata ataaattttc tgcaaaagag
    10141 tatagtaaaa atataaataa agctactaaa tatgcttttt tatctttttc tataaagctt
    10201 tctattcctt tatacatcaa atacataaat aatatacaag caactactcc tagtacacta
    10261 attatatata cataagaact atctaatgga ttagttgctt ttatagcagg actatatgaa
    10321 tttccaaaag gttttagaaa gttccccttt tcagccaagt atacatgcca gaacttaggt
    10381 ctacttgcta ataatctatt taataaagtg cttttatcaa atactgtacc aataattaca
    10441 ctaaataaag taagtattat cggtaatgtc tttaggagca ttggtagtcc ttttattttt
    10501 tttatatcta tccatcttat tatttctaca aatattaatc ctgctaatat agtgaaaaat
    10561 ccagttctac tatatgtggt ttgataaaca aaaaatgcag aaccaaataa aattattcta
    10621 tcccatttat tataatcttt atatcttaag aatatatatg ctgcatatat agtaaccata
    10681 tgataaaata cactatttgg attaccaagt ccaaaatcca ttcttgtaac aagcatatct
    10741 attttttcta tatatctata gtggacttcc tgattaaata ttaatgaagg aaatagatta
    10801 aataataata tagctaccat taaaaaacat gccgatatta ttaaataatt tttaactata
    10861 aatttatcat ctatttgctt agctgataca gctaataaaa cgattaataa tatagctcct
    10921 tccctattta ataaataaag taagacacaa actccaacta gtaccatttc tgttatactt
    10981 tttctcttat caaataaaaa aagtagcatt agtattccta ttgaagaata aaataacgct
    11041 gatttaatca tccctatatc catatttggg tcaataaatg atatcatata ctttctaagt
    11101 aatatgataa acaaaacaaa tatataaaga tattttctaa tcataatatt tctatcatca
    11161 tttaaagtct tcattttact ttctcccttt tagcttcgta aagtattact tgcaaatatt
    11221 ttacaacact tccatatctt atttacacct gttaagtgtt tgctttctaa gaaataattt
    11281 ctctttttag cccaactttt tccccaccaa tgtatcccaa aagtgttttc tgttatacat
    11341 tctggtgtaa agtcttcttt aaatccaaaa ggataaaaat attctttgtc gtaaacacgt
    11401 attccttctt cataattatt tgggtcaatc ttcttataat tcttttccat aagatgagtc
    11461 attatctttg gtattgtaaa caatgaagaa gtaagtactt ctttttcata atactccaat
    11521 aagtctttta aaaaaggatg tcctttttct acacctatta tagcaccatt tatatactct
    11581 ctatcatctt catacccaca gattagccta ttattttcta aaagaggagt tatatccttt
    11641 aatatttgca tatctgtatc catataaact ccaccttgtt catagagtac ttttattctt
    11701 gtataatcag atatgaatgc ccaaagtttt cttttataac attcttccaa aaacttattt
    11761 ccttttattt ctttctctat atcaaagttt ttttcattcc actctactat ctcatattct
    11821 ggaagctttt ctttccaaga atttatacat atattttcta tatttccttt tggaccacca
    11881 aaccatacat aatgtatttt tttaggaatc aaatgaacac ctcctacata tattcttgtc
    11941 tatcttcttc ttttaactgc tctactattt ttttgacatc ttgagcttta tcttttctac
    12001 aaaccataac tgcatcattt gtagatacta ctatcaaatc actaatacct attgtcgata
    12061 ttaatttgtc atctgaatat ataattgaat ttttagtatc tatagtaata ttttcacctc
    12121 ttttgatatt accttcatca tcagttggat atattgcacc tagagaatcc caagaaccaa
    12181 catcattcca tccaaaatca ccaggaacta ctataacatc attacttctt tccataatac
    12241 cataatctat agagattgat tgtatagttg ggtatatttc ttttattttt tccatttctt
    12301 cttttgttcc taagtattta cttatatctt ctaatttttc atatactttt ggcaagtatc
    12361 tcttaaaatc ttctagtatt ttagatacct tccatacgaa cattccacta ttccatacat
    12421 actttttaga gtttacatat tctttagcaa tttcatagtt tggtttttct acaaactcca
    12481 ctacctcata tgcaacatct tctatagtat tttctctatt aaaatttatg tatccatatc
    12541 ctgttgatgg aaatgtagga gttatcccaa tagttactag tttataatta ttttctgcta
    12601 tatagatagc tttttccaaa atagatttaa attctttttc atcttttata taatggtcag
    12661 ctggatatac acacataaca ccatcaccat attttttcat tatgttaaat gctgcaaagc
    12721 ctatagctgc tgctgtattt ctggcacatg gctctggcaa tatattacta tccaaacatt
    12781 tatcttttac tatatccttt aaagcttcca attgcttctc attggttact ataaataaat
    12841 catctttttt tgctagagaa tctattctgt ttatagtttc gtttattaaa gcatcttctc
    12901 cacttaaatt tataagctgt ttaggtactt cttgtctact aagtggccag aaacgagttc
    12961 ctcctcctcc agccattatg acattgtata ctttcattgt ttatctcccc ttagcttttt
    13021 atatagaatc cactacattt aatatataag atattaaatt gttcaactta aattctacat
    13081 cttcttgatt gtctgaattt gctccaaagt aaaatttaat tttaggttct gtacctgaag
    13141 gtcttatagc tatccaagaa ccatcttcaa gtaaaaattt aagtacatct gacttaggta
    13201 aatcatcaac accatcttta tagtctaaaa ttttatctac ttttacatca gccacattat
    13261 ctattttaac acttctaaag taaagcatca tctcttttat tttttttatt ccatctatgc
    13321 cttttaaagt tactgacttt aagtcctctt tgtagtatcc aaatttatta tatgtatcta
    13381 tcaaagcttc atacaagttc ataccttttg aagaatagta tgctgccatt tcacatatca
    13441 taagtgaaga tacaacacca tccttatctc tagcatgagt tccaattaag taaccatagc
    13501 tttcctcata tcccattata aaacttctat cattgctttc ttcaaacgct tttatttttt
    13561 ctcctataaa tttaaatcct gttaaagtat ttaaacaatc tacattataa gcctttgcta
    13621 tttttgcacc tagttctgat gtaactatag tttttattat tgtaggattg tttttaggaa
    13681 gtttattttc ttcaactaaa ctctctaata tatatctaac taaaagtgaa ccgacttgat
    13741 ttccatttaa gactacatat tcaccacttg aatcttttac aacgatacct actctatcac
    13801 aatcagggtc tgttcctatt atcaaatcag tgccattatt ctttgccatt tcaatagcta
    13861 tattaaacac tgacttttct tcaggattag gatactttac tgttgaaaaa ttagaatctg
    13921 gcaattcttg ctccttaact accattacat tttcaaaacc acattcattt aagacttttc
    13981 ttactggctt atttcctgta ccatgaatag gtgtatatat tatttttaat tttttcccgt
    14041 attcatctat tatattttgt cttaaaactt gcttcttaac agcttttata aactcatcat
    14101 ctacttcatt atctaatata gttattaaat ttttagataa agcttcttca aaatctatac
    14161 ttttaatagt actgtaatca tcaatcttat taacttctgc tattatatca tttgccatat
    14221 ctatacagat ttgaccacca tctgagtcat atactttata tccattgtat tctttaggat
    14281 tatgacttgc tgttattact attcccatag cacactttaa atatctaaca gcaaaagaaa
    14341 gttctggtgt aggctttaat tcttcaaaaa tataagcctt tacaccataa gcacttaaag
    14401 tctttgctac ttctatacaa aactccctag acatgtatct actatcatgt gctataacta
    14461 ttcccttttg cttaccttct tctccttgag ttttcatgat ataatttaaa acgcccagtg
    14521 tcgcacgtct aacagtataa atattgattc tattagttcc cgctcctatt atacctctta
    14581 aaccacctgt tccaaactct aaatttttat aaaatctatc ttgtatttct ttttcatcat
    14641 cttttatact taatagctca tttttcgttt gttcatcaaa ataaggagaa tttaaccaca
    14701 tctcataatt attcttataa tccatatttt catcctttct gactctaatt taaaatttca
    14761 aggtaattat ctatcttcta agtagttttt gttttccttt tcttaaaaat tcaaaagcta
    14821 aatccatctc ttctacctta aataaaacta tcaatacagt atatacagaa gctccaccta
    14881 atactccagc tccaactgat ataatttctt taattgttcc tgaacccata aatgcatata
    14941 gattattata tatcaataaa gtagctactg ccatcacacc agatgctacc aaacttttta
    15001 gaccagtctt tataatttta tcccctccaa aatatccatt tttcttttta agagatataa
    15061 ataataatat aactgtaatt atgtttgatg tactagttga tatagcaata cctgcatgac
    15121 ccaatggtct tatcaatatt aaattaagaa ctatatttat tattaaagct atacttccat
    15181 ttatcattgg cgtttttgta tctgaaagag agtaaaatgc cctatataag acatctctta
    15241 ctccacaagc caataaacct agtgaatata atctaagcgc tatagatgta aggtctgttg
    15301 ataacgcatc aaatttaccc ctttggaata atattctaac aagaggctct gctaatataa
    15361 tagctcctat agatatagga agcaccacca atataataca atttgaagat tttacaatag
    15421 tgcttataaa tccttcttta ttatctttac cagacatttt tgctagttta ggatatataa
    15481 ctgttattat agatgtaaca aataatgcca taacaaactc atttagccta tttgcatagt
    15541 taagagcaga caactttcca tcacccaaag tggttgccaa taatcggtca acaaatatat
    15601 ttaactgtcc aacagctact cctaccaaca taggcaatac taggttgact aactctttta
    15661 tagactcatc ctttaaattt atatatggct tatacttgta ggacttctta aacgcaaatg
    15721 gcagttgaaa caatagctga ctcgccatag ccaataatgc tcctgctggc agtatccata
    15781 catttttacc tgcacttagt gctattgctg ctattattat aatattatat gggattccta
    15841 taagcccagg aattacaaaa ctgtcattca cctgtaaaaa cgaactaaat atcttacttc
    15901 cactcaggaa taaaactcct gaaatcatta ttttagtaaa taatattgct gcttggaact
    15961 tagcagggtc gtttctaaat cctgctgcaa atactgtaac aaattgctct gtaaataaaa
    16021 gcccaataat agctattact atagccatta tatagcatat atttaaaaca ttattagtaa
    16081 attttaaggc accttcatct ccgagtcttt tctttgtctc aaagtacata ggtatataag
    16141 ttgtagcaac tgcagagcct atgactgcta tgattaaacc aggtatattt aatgttaata
    16201 caaatacgtc tgcatacata cctgtcccat agaaatttgc aagtacaagt tctctaagga
    16261 aacctaatac ttttgaaaac atagtggctg ccataatcca caatgctgct ttagctgttt
    16321 ttgacatctt ctttctcctt atctaattta aactctaaaa gtagaacttt taaaactgct
    16381 tataaacatt attctaatta agtaaattta acctatgaaa agcttcctta taaatcaaaa
    16441 actatttcga ctcatatcta agtgccctaa atgtaatcaa tagtaataat agtattaata
    16501 ctatacaaga taagaatgtt agattaaata tgctcttaaa tagactataa tcacctactg
    16561 tatacccaac ttcaactaca tcatttatat tgctatcttt caagaattta tcttgtgcac
    16621 gacttaagtt atcactacaa atcagtattg gcatattttc ttttgctgat actactccca
    16681 aagaaatata ttcgtttaca tcagatctac taccaaagtc taatctagca gtatagattt
    16741 tctttatatc tttttcatca taaaacttct ttattaaatc aacatttgta cttgacttat
    16801 ttatttgctt tattccttct atatttttta agttagaatc aatctctgca ataaactctt
    16861 ttgaatcacc taatgcatat atttttttaa gattcttgcc ttttaagaaa tcaatttgtt
    16921 cctcaaaccc ttcatcttta gcccatatta ttggcattcc acttttagcg gcataagaat
    16981 atacagatat ggcgttctct aatcctgttg ttgagctaag taaaaatgct tcatcaaatc
    17041 ctactgttct atacatttct ctagctatct ttaaagatgt atcatatctg tctttccctc
    17101 ttattctttc tactttcatt cccatttttt ctatattacg ttcagcatct ttagatactg
    17161 cctttaaacc acctactatc gtaaccttct ctggacctag ttcttttaga taatttcttg
    17221 ttactctacc aagattttta ctcttagtta caactattgg agcatttttt gcatatgcta
    17281 aaggtgttgc acttatacta tctacaattg actcttcatt tacaagaatt acttctttag
    17341 aatccttaaa tgctctttta tttaattctg tcgaaatctc tggtatactc ccttctatca
    17401 attctctttt gggatttgca tctgcaaata ctcctatcgt actagaaata gagattaaca
    17461 aaactaacga taatatagta gaaatttttt taatcataag ttctcattcc ttttcttttt
    17521 tgtatttttt gacaataaaa aagctctttt taaagagctt tttcattgat tagtaatcta
    17581 atattttaaa attatctagc tccgtctcct gtcaatacta ctttaacagt tttaaagagt
    17641 atcttaaagt caagtaatat actcctattt tttatatagt aaatatcttc tttaagtttt
    17701 tcttcaggag tgatttcgta tccaccattt acttgtgccc aacctgtaag cccaggtttt
    17761 atagcgagcc tgttgataaa tccaggtatt tcctcattaa attgaacagt aaagttcggt
    17821 ctctctggtc taggacctat taagcccatg tcccccttta atatattaaa cagctgtgga
    17881 atctcatcaa ttctagtttt cctaataaac tttccaacct tagttattct aggatcatct
    17941 ttttcagccc attgtgcgcc aaatttttct gcgtctgttc tcatagatct caatttatat
    18001 atatagaatc ttctcccgca ttttcctagt ctttcttgct tataagttat tggtcctttg
    18061 tcctctattt taataagaat tccaaatatt gctattaaag gtaaacctat cacaagtcct
    18121 attatagata aaactaaatc cataattctt tggtacaagt cgaataaaac tgttcctttt
    18181 actatgctat agtcaatctc acttgcaatt tctttatctg cccctggata gcttttaatt
    18241 ttaggtattt cacactcatc attagtatat ccagacatag ttacctccat attggaataa
    18301 aaattttttt aattcttaat ttcaacttat ttctatctta tttttgactt atttctatat
    18361 tatactcaat aatgagcaga ttatcaatac aagaaacatc attgtaaaca atttgattct
    18421 ttacaaaaat atatcatctc ttgttatatt ttacattata acaggagatg atatgttttg
    18481 ctttaatatt ttctatttta ttttactgta tcttctattc ctattccaat ttgagttccc
    18541 attgttttta ctttttctgc agtatttaac ttatccatat cgctcttatt gtcaataaat
    18601 gcacattcaa caagtattgc tggataattg ttatttctta acacatatag gtaatctttt
    18661 ccattatcaa gtgttcttgt cttgattcct ctatttttca tgttaaattt ttctagtatt
    18721 ctttttaaga tatttgatgc tgcagtctta gttgttccac catttttatc ttttactttg
    18781 taataaattt ccacaccatt accagctcca ttagacgcat tgtaatgtat acttgtaaat
    18841 aaatctggct ttatagtatt tgataatgct gttctttctc ctaaagccat tgttttatct
    18901 gtatctctag tcataacaac atttatacct tttgaacgta aatattcagt agttgctaat
    18961 gctgtattaa gagtatattt cttttcttgt gctccaccat ttaatccact tgttgcccct
    19021 gaatcagaac ccccatgacc tgggtctatc actacagttt ttcctgctgc tgaacctgaa
    19081 ttatctggtt ctgttggtgc attgtgtttt gataaactag atgctataga atttataaca
    19141 ctatctttca ttccatcacc aatttgatat actgtttctg ctgttttttc tgataagttt
    19201 gtactatggt atgcagaaac ataagttttt ggagttataa gtattggagc ttttaattta
    19261 gctgctaaag gtcctgctgt tattgaatca actattatgt cagattttgc aactaacatt
    19321 gcactcaact ctttatctgg atagaaagtc ttaattacat ttgcatttgt ttcatgtcta
    19381 tctgctccag aaactctatt ttttgaagta ccattttttg ctattttaga tatttggtct
    19441 ataatttttg atgataaact ttgactacca cctatgtaat aagcttcttc taaaccttgt
    19501 gaagataacc aattatatgt accttgtggt acagaatctt tattagctaa tattataggt
    19561 tgttggtctt caccagcttt tgatgaaata ttaagagcat catattcacc agcatatcca
    19621 tttgctatgt atattttatt aacatcatga tacttatcta tttcttttgc tataagtaaa
    19681 gatgtttcat gtctatcaac tccaccaata cgagtcacat ttacatttac agcagattta
    19741 atttgagaca ctgccttttg tgaaactgaa ccatcatcac ctataattat tacatcactt
    19801 ggatttagac gttttaactc tgcttttgta ctttctggta tatcatcttt atttgcaagt
    19861 aatattggtg cgtcatatgt agatgcaagt ggagtagctg taattccatc tgctgctaat
    19921 tctccattaa ctattacaac cttactagac ccatctttcc aaccctgttt acttatctca
    19981 acagcagttt caaatctagt agctccagca agttttttta ctggtgcaga agtttttctc
    20041 atatattcat ttactgcttt tgttgaattt gcatcactta ttataaactc ttgtgtaaca
    20101 tcataatcct taagattcaa tcccatagac tctaagtctt ctttgtttaa aactttctgt
    20161 tcatctttac catttatttt tatgctttga agttcccata catttaagta ttctgggtct
    20221 ttgttatagt ttattacacc ttcatatgaa tctgtctttt cattgtaatt taaagtagct
    20281 tcaagtggca tatcataaca cataaaattt aatgttattg aatcagcatt taatttttct
    20341 ttaaatttta cacttgcatt agatttactt cctaacacta cactttttct ttgtaaattt
    20401 atattaatat ctgaattaga ttctgtattt gcagtagtat tttcagccgc taaagctacg
    20461 tttggtgcca ccattgctac agacaacatt cctgttgcca ataattttgt tgtttttttc
    20521 atcatatccc ccctaatttt ttggttcttt aactctttaa atctttttta atacatctta
    20581 agcttgtttt atattacata aattcaatat tatgtaaatc ttttttcatt atagcacaaa
    20641 aaattacaaa ttgtaaactt tttgtaatct tttatggcat ggtttttaaa ttttatcgag
    20701 gagaaatttt gattatattt gatagtttcc gtatatttga acaaatcaaa gactttatca
    20761 tctaaaaata ttgcactaaa ttttaataaa tatttttttc ttgtacatta tagacattac
    20821 tataacccag aataggacat acaataaaga aaatataaga gaatcccaag ttgtacctgc
    20881 ccaaggtgtt ataagttcat atgtagtcca ctctaccaag gtagttggat aatcgacttt
    20941 gttcgtaagt tttggaacat tccaaaaaac atgactcaat atctcaaggc acatatatgt
    21001 aaaaatgggg ctacttccaa gtgctattat tggggtaaat attttacttt tatttttgat
    21061 gtcgcatata aaataaaata ttgacagtaa tataccataa ctgccagcca ttaaaagtac
    21121 aaatgagcta gaccataatc ttttattaaa tggaaaatac tgattaaaga taaaagcacc
    21181 tattagcagg attatgctta taaccagtat tttaaagaat tttttatatt ctcctatgtc
    21241 ttcttttaaa agtacacatc ccatagtgca tcccaacata cctgaagaaa ttgctactat
    21301 actagttaaa aaaccatctg gttcaaattc cggcttgtac aaatgtcctt tgaagaaatg
    21361 taaatccact aactgtgcaa gagaaccttc tagctcaaat ccataaggtt ttgctaagat
    21421 gtagtaccca actattataa atgtagatat acatagaaaa gtaattattg tagcagtact
    21481 tccaacattt aattttttta gtagtaaata tactaaacta gtcacaaaat agactaatcc
    21541 catacgttga agtactccaa gtattcttac agtatctaaa tcaggatttc ctaggtaatt
    21601 taagaaaaat ccaaataata tcaataagat acttctcttg aatatactaa gtattatact
    21661 cggtgtggat ttattatttt ttagtttact atttattgat attggaattg taacacctaa
    21721 agatataaca aaaaaaggaa atgcaaaatc tgcaagtgtt acaccatgcc atacagcatg
    21781 tcttaattga ggatacattc tcatccaagt cccaggatta ttgcagacta tcatgagggc
    21841 tattgatagc cctcttatta tatctattga tttaatccta ctattcataa gtttagaatc
    21901 tacagcatta tttgaaatt
//
LOCUS       DBGJFF010000061        21628 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.062, whole genome shotgun sequence.
ACCESSION   DBGJFF010000061 DBGJFF010000000
VERSION     DBGJFF010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21628
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.062"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            139..768
                     /locus_tag="ACOIAF_002256"
     CDS             139..768
                     /locus_tag="ACOIAF_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669265.1"
                     /translation="MNKYLLINPVAERMYGEKFHLIKANLIKKGYIITECEPQLEYVK
                     KQYKEYTKKTENTMLDCRCPESISLLKRNNLINNFEVPMIEPILVRTCRVLYDKYIKS
                     KDDMLVVTCPCTQLRDFASEKFKDKGNAMFYTWKEFAEQEGVKSLGKIDESPIPLGYF
                     KDTFEKVLEVSSEDEILSEIQKIRNGFEDKYDIVEMFYCKDGCNNGDGL"
     gene            769..1551
                     /locus_tag="ACOIAF_002257"
     CDS             769..1551
                     /locus_tag="ACOIAF_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2669266.1"
                     /translation="MVNKFNNKFLRLVKSVLIFFIILLLWKITNYLGIWSDYILPSPE
                     KVYSTFLNMISDGSIFINVYASMKRVLIGFAISTAIGVPLGIFFGIYSGVYEYFKSLI
                     NFLRNTPPLALIPMLILWFGIGEESKIIIIVLASFFPIFTSTLKGIKNCDSKLIEVGR
                     VFEFSKLQIIFKIIIPNAILDIAVGLKLALGYSFRAIIGAELVAASSGLGYLISDGKE
                     MSRTDVVIVGIIVIGLLGIITDYIFSIIVKKVSKGKMVEAYE"
     gene            1544..2422
                     /locus_tag="ACOIAF_002258"
     CDS             1544..2422
                     /locus_tag="ACOIAF_002258"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2669267.1"
                     /translation="MNNLVCDSGYIIRNLYKSYKVDNKEHVVLSDISLNIDRNHITVI
                     LGESGCGKTTLLRAIASLEGINSGEIRFINDNKEYRPNVGFVFQESRLMPWLNVSENI
                     AFHNQRKNTILNKVLRKFKHNKRIRSKIINDNFRSLETNDNLNKSNKYNRKSNIDVEK
                     YLKMMNLQNFKNSYPNELSGGMAQRVSIARALSFNPDMLLMDEPFSALDYFTRIDMQN
                     EVIRIHKSTNKGVIFVTHDIDEALKIGKKIVVFTDERKVKEFNIEDGYNRDLTSDYYI
                     KLKKDILMTMKSNKGI"
     gene            2455..3414
                     /locus_tag="ACOIAF_002259"
     CDS             2455..3414
                     /locus_tag="ACOIAF_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGZ2669268.1"
                     /translation="MKLKKLLIGITILTLATASLAGCAKKNNGEKLSEINLTYVKSPL
                     NVPSIIQKQDDLFGKEFKKDNIKVNFHEITTGPEQTQALAAGEIDFLHALGGTSALIA
                     ASNGVELKILNTYSRSPKGFMILTNNNSIKSAADLVGKKVAGPKGTILHQVLISALDK
                     EGLSMDDVEFVNMGIPEASAALSDGSVDAALIAGPAALKAMKSGSKLVANGEGLVDGI
                     IVTAVSTDFAEKHPEIVERFMKVEKETLEYVNNNFDEAMEKVAKEVDLSLEETKELYA
                     WYDFSLDITDKDISSLEDTQDFLIKNKLQEKKVNIKELIYNAK"
     gene            3728..4420
                     /locus_tag="ACOIAF_002260"
     CDS             3728..4420
                     /locus_tag="ACOIAF_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HGZ2669269.1"
                     /translation="MSYKVYIVEDDISISTLLKDYIEKYGFEVIVEENFDDIMITFES
                     FKPHLVLLDVNLPKFDGFYWCRCIRQQSNLPIIFISARDGNVDQIRALESGADDYITK
                     PFYYDVVMAKIKSHIRRVYGEYSPKIDERIVELDGLKFYPERLELELNGENLIITKKE
                     GILIECLIKRYPKVVSRDYLLEKIWDDIEFVEENTLNVNVSRIRKRFEELGIDNAVQT
                     VRGLGYKLNKNW"
     gene            4661..5434
                     /locus_tag="ACOIAF_002261"
     CDS             4661..5434
                     /locus_tag="ACOIAF_002261"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGZ2669270.1"
                     /translation="MYLGSSFLGRNISVILKQQHKLYQSMIQEHKKIHSEHLTFINQW
                     IHQMKTPISVISLQLQDYEGEEVTENIRKEIDRLNKGLDMAMHFARADNFQKDFIVEK
                     AKLNEIVSSIVNEEKRLFIKSGMIPKIQVDKFTYVYTDVKWMKFIIGQLITNGVKYSK
                     DYSNYLTIKSEENNKYVILSIIDEGIGIVSKDLKRVFDPFFTGENGRKFGESTGMGLY
                     LVKKVCDDLGHKVSIKSEVGKGTEVSIYFNKDVNSLIKV"
     gene            5682..6533
                     /locus_tag="ACOIAF_002262"
     CDS             5682..6533
                     /locus_tag="ACOIAF_002262"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HGZ2669271.1"
                     /translation="MSLDVIFILKSVIIAIVEGLTEFIPVSSTGHMILVGNLIDFKGQ
                     FAEMFEVVIQLGAILAVVVLYWKKIKDSVIEFFKFIFTGGKEGKIGFRFGMNVIIGCI
                     PFAIIGVLFYDNIKSLFNLQSVIIGFIVGGILLLVVETLFRKKNHSTDNIDKITPIQA
                     LKVGTLQVLSAWPGMSRSASTIMGGWIAGLNSPTAAEFSFFLAVPAMVASSGKDLFEF
                     DYSIMTPTLWIALVVGFIVAFIVSIIVMEKFVNFLKKKPMRVFAVYRIIMGVVLAVLA
                     FTNIISV"
     gene            6637..7383
                     /locus_tag="ACOIAF_002263"
     CDS             6637..7383
                     /locus_tag="ACOIAF_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2669272.1"
                     /translation="MKILTVNNLSKVYGKKIIFNALNDINFSIEDGEFVGIMGPSGSG
                     KTTLLNMISTIDKPTTGTMELKGKNPLLLRGEELALFRRRELGFVFQDFNLLDTLTIG
                     ENIVLPLTLDKVSVKEQDERLNEVSTILGIKDLLGKRTFEVSGGQAQRTAIARALINN
                     PSILLADEPTGNLDSKSSKVVMELFQKINKENKVTTMMVTHDPLAASYCSRILFIKDG
                     SIYNEIYKGSSREQFYQEIMDVLTLLGGDN"
     gene            7383..9299
                     /locus_tag="ACOIAF_002264"
     CDS             7383..9299
                     /locus_tag="ACOIAF_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HGZ2669273.1"
                     /translation="MNIKQFAINNISRNIKAYLGYLASIVISSSLLFSFIMFTSHPDL
                     DVSQFPDYLKEGLKMSKIIAYLFLFFFVFYSVSVFLKSRYKEFGILYITGISKRQVMK
                     LIFIENMIINIVSSVIGIIIGLIFAKIFLVVMSTFLELSPLKFYIPFNAILSTLIYFM
                     ILAVLTSIFTSFIVKENQVLKLLKGTKTPKPEPKTSLMLAILSICLFIIAYYNAITST
                     DQYTTYLRLVPVTSLTIVATYLFFSQFSVFFMKKLKLNKRFYRKNTNMLWISNLIYKV
                     KDNARIFFLITITSAVAFTAIGTVYSFWKDVERQINLIYPNTIYYSTMTLHNDTKKPD
                     SKEKDKERMMFIENNLKKENVDYTRINGEFKTVFPKKSDFNVRIMKESKYLEITETIG
                     IKPISFEDNECISLLSSSLPGNKNVKENIIVGNRGLKVAKQVEECVMPAYYKNMYVVK
                     DNFYDSIESKFIVDRFTAFEVEDSSKTIEICREFENKFNNESGMQPYLFFSKELMIGT
                     GKLMYSTFIFLAIFIGLIFFVTTSSFLYNKFYMDCQVDKKKYEQLNKLGMTYNEIKKA
                     STIEIGIVFLFPYVVAVIHSIFALTALKNSFDIEVASSAVLVMGSLFLVQIVYFLIVR
                     NNYLKEIRLNLVNF"
     gene            9582..10496
                     /locus_tag="ACOIAF_002265"
     CDS             9582..10496
                     /locus_tag="ACOIAF_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HGZ2669274.1"
                     /translation="MILIQSRGKMKCKELSEELEVSERQIKSYKMYLEQAGIFINSTP
                     GIYGGYEIDKCNSISLIKLLDSEVSILDMINSQLEYNNDIYKNEFNNIVEKIKAVLNT
                     GEKSDTYMDYFTVQAQRNCDYESEKNKCNEIIRAYTTKHKFWIEYYSLNSGNSERIVH
                     PYGLFNYKSDTYMVAFCEKRFKFIDFKLCRIKDYKVLEEKYNVDKSFSWDEYSKNSIG
                     IYKGEEINVVIKISHPFSTIIKEKVWVNNQQIIEYDDKSIMFKAKMRGYEEIKSWILS
                     MGAYVEVVEPDRLRNDILSEIEKMKKIY"
     gene            10574..11311
                     /gene="cas6"
                     /locus_tag="ACOIAF_002266"
     CDS             10574..11311
                     /gene="cas6"
                     /locus_tag="ACOIAF_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HGZ2669275.1"
                     /translation="MKMKIEFSTGCIPISYNSLFMSIIKEAIRKSNEDYYKNMYYYKE
                     KNNKKTKNFTFSVYIKKYSIEGDNFIIEDKVILNISTPDLELGFHIYNGLMTSKKCLY
                     KDYELTRIRIDLSREKKVTEERVLFNALSPICVKSKEGKFLEITDDRYIEELNYITNE
                     VVKNYRGNGLKRKLEFENVGFKKVVVKESLREFKKITGKEYQYINGYKGKFILKGDID
                     DLNLIYNLGIGFRRAQGFGDVDILEWR"
     gene            11317..13194
                     /gene="cas8a1"
                     /locus_tag="ACOIAF_002267"
     CDS             11317..13194
                     /gene="cas8a1"
                     /locus_tag="ACOIAF_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas8b1/Cst1"
                     /protein_id="HGZ2669276.1"
                     /translation="MKLRVYRGDWFFNMGIVGFLNIIKKADKQAEIFIMEDYIEFDSL
                     FLENFHEYYFNYFMDEYDVSKRIKKNIDYSINFIKSKPDRIKDGIKKIKDSVKQQNDK
                     IKKFDEEKFNLIKEKLDSMSKIKSYDEFDSLEALVDETIDIFKIKSINDRLTANLYKY
                     VVQDNYFGQVSFFNVAKAKLDLDGLKQVMFNDYLRQIIYFGELEDLLEENDYDKLKNY
                     LNDRLNSIDKDINEKKVSKSSINTIEKIMKEINKNFIKKNKSIEDIKEYMDSLEVCEM
                     CGLYKGILDEYSESNFAPLGVSTNNARNMFWNQAYVPSICDICKLILFCTPAGATYTR
                     KNYLINEENEFYLFVNMDTCINELFERNNSLKVQKSESSDSKDENPFNQLIKSIVEEN
                     TLKSEWQLRNILFVEFKASIDSKKCKINYFNIPTYLAKFFTDKYANKKIQSIYDYKLK
                     SNVLDLLLKNRDLKHITNNILRNKVKNDMESNNKSNISGIDCFRVVQLRVLINSYKKG
                     VYKMDSKNLEKNDEKLKIIYYLGCDIHDYFVNKNSKNKIDGVSYKLLNSVKVGNKSDF
                     MDTIIRVFMSAEKQIPAFILDIEIEKDLDFESIGHAFISGLISGKYEGKDKLPNEKEE
                     K"
     gene            13196..14110
                     /gene="cas7i"
                     /locus_tag="ACOIAF_002268"
     CDS             13196..14110
                     /gene="cas7i"
                     /locus_tag="ACOIAF_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein
                     Cas7/Cst2/DevR"
                     /protein_id="HGZ2669277.1"
                     /translation="MKRGLTFTVIAQAQSLNYGEGIGNISELKKLSRDDGNMYTFASR
                     QCLRYDIVRLAAELFNWNLQVVDKEKGTIQFKDNISIRESEEMDLFGYMKTNKKDEKD
                     KKGGSLTREATVRLSNAISLEPYRSDMDFLNNKGFADRIGEHPNLANIEQHLSYYTYT
                     VTIDLSKIGKDGEIELDNKEKCRRVVEFLEIIKVLNRNIRGRQENLSPLFVVGGVYEI
                     ANPFFLGRIKLKGDKNGFKINKQAIEEVIQGTFLGKDLKEFTYVGMVDGVFINKEEFK
                     GLFEDNFLSVDKFFGQLVKEVKEYYGVN"
     gene            14112..14813
                     /gene="cas5b"
                     /locus_tag="ACOIAF_002269"
     CDS             14112..14813
                     /gene="cas5b"
                     /locus_tag="ACOIAF_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas5b"
                     /protein_id="HGZ2669278.1"
                     /translation="MKVLKLDLFQETACYKKPFAMKITETYPLPPYSTINGLIHKILN
                     ATEYIPFNISVQGTYESIFNNYQTTYFYKKDSITSMPMNSHMLLNVNLIIHIGGDFDL
                     LEEIYDSLLNYDEHLSLGRKEDLVRINDIRFVEVNKFEVDEDIQLDDYENYKLSECNI
                     RMPIYIPKTSLQDTDIDGISYRLNNYYDNNAVEDKKRVWNKVDSYYVEDGNTISIGDI
                     LLDNEGDLLYFNNIN"
     gene            14824..17145
                     /locus_tag="ACOIAF_002270"
     CDS             14824..17145
                     /locus_tag="ACOIAF_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HGZ2669279.1"
                     /translation="MLYAKSNPVETLREHTDELLKQMNVLRESYGKNINSLDFLEEEI
                     FWQLLDFVIEFHDIGKAFSPFQELIKSRMDTKINEPKIVTHLENNVGHNYLSPAFIDY
                     SYIDRKKNKELRAVLNQVIVYHHERDIFIDKDFKNLIQKILDEDLINKVYELQHEFKV
                     RYPIKTEKLSKVYLQSVEKRIDKNHKYYNLYIMLKGILHRLDHSASAHEIVECNSNMN
                     IGEYTEKHLVKEFGSLREVQSFAKSNRNKNIILIASTGMGKTETALIWIDKDKAFFTL
                     PLRVSINALFDRAKNIIGVGEANDTFLGLLHSTAIDYLEESNQENSNEIVDLAKLLSC
                     KLTFSTIDQIFKFPFLYRGYEKVYSTLAYSKVVIDEIQAYSPEIAAVLVKGIEMIHKI
                     GGRFMIMTATMPTIYIDELKKRGVMNSNLADLTCNTEKIRHCVSIVENSIDENLGKII
                     QNGMNKKVLVIVNTVKSAVEKYELIEEIVKPKGIDVNLNLLHSMYIQEDRAKLEKYIK
                     EFADSDSNGIWITTQLVEASLDIDFDELHTENSTLDSLFQRFGRCYRSREYEENSPNI
                     FIYTEKATGIGSIYDKDIVEKGLELLKTFINGKECVKMKEKYKVEMVKILYSKESLEG
                     TAFEKRFTSALNILDTITPYSLGSKDAQNILRNIEGYTVIPEDIYNKIEETLIKDYEE
                     LGQELINAYSNDDRQLINKIKSKRKKARREIIKKTVNLPIYKAKQNVIDIQVKGLEDL
                     KILLYKYDIYENKHTKQIFGKGVLISNEIDQFI"
     gene            17193..17711
                     /gene="cas4"
                     /locus_tag="ACOIAF_002271"
     CDS             17193..17711
                     /gene="cas4"
                     /locus_tag="ACOIAF_002271"
                     /EC_number="3.1.12.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HGZ2669280.1"
                     /translation="MPIDLEVLKVQGVKINYYYVCKRKLWLFSKGITMEQNSSRVLSG
                     KIVHEDSYKRMKNKEILIDDILKLDIIKDEYVKEVKISSKMKEADEMQLYYYLYYLKN
                     SMGIEKKGTINYVKEKKQEELILTEEKERIIENTLININEINMQLSPPKIEKKHYCTK
                     CAYYEFCFVKEE"
     gene            17714..18694
                     /gene="cas1b"
                     /locus_tag="ACOIAF_002272"
     CDS             17714..18694
                     /gene="cas1b"
                     /locus_tag="ACOIAF_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated endonuclease Cas1b"
                     /protein_id="HGZ2669281.1"
                     /translation="MYKDYYIISNGDIKRKENTIYYYNLEGEKKILPVEQVDDIHIYG
                     EVDLNTKLINYISKYGVMLHFYNYYGYYSGTYYPRKKNVSGFSLVCQSACYLDKNERL
                     YLAKSFVESASYHILRNMRYYKVDESLIDTIKDELNDLNDVKEISELMGKEGRIRKTY
                     YKSFNQILKDDFEFYKREKRPPKDPVNALMSFGNSIMYTNVLSEIYKTQLDPTISFLH
                     EPSTKRFSLSLDIAEIFKPIIIDTIIFSLINNKRINKNDFEYQDKICYLSEKGKKKFL
                     QEFENKMQTTIKHRNLNRKVSYRMFIRLECYKLIKHFINDERYKVLKVWW"
     gene            18697..18963
                     /gene="cas2"
                     /locus_tag="ACOIAF_002273"
     CDS             18697..18963
                     /gene="cas2"
                     /locus_tag="ACOIAF_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HGZ2669282.1"
                     /translation="MFVIVTYDIVEARSLNRIRRILRKYLTWTQNSVFEGNITEGKLH
                     KCISEIENIIDNSEDSIYVYEIKNPNSIKKKCYGIDKYSDEMFI"
     repeat_region   19147..20756
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=19147..19175
                     /rpt_unit_seq="gttttatattaactaagtggtatgtaaat"
     gene            20946..21374
                     /locus_tag="ACOIAF_002274"
     CDS             20946..21374
                     /locus_tag="ACOIAF_002274"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HGZ2669283.1"
                     /translation="MIKKLNSKDINKIMEIWEKSTIKAHDFISKEYWQNNYNTVKNEY
                     IPISDTFVYDDGDEIKGFISIIDKRFIGALFIKPKYQNLGIGGKLLDYATKKYKSLSL
                     AVYKDNKKAVVFYNKKGFNIVKEQVNEDSGFKEYIMEYSK"
BASE COUNT         8811 a         1918 c         3722 g         7177 t
ORIGIN      
        1 taatatttca tttaaaatat taaaaaatgt agataaaaat tgttttatta agaaaaaatt
       61 aatatattga caaaatttaa gtgctcattt aaactattaa ttgataataa tattcaatat
      121 taaataatag gaggcgttat gaataaatat cttctaataa atccagtggc agaaagaatg
      181 tatggagaaa agtttcacct tattaaagca aatttgataa aaaaggggta cataataaca
      241 gaatgtgaac cacagttaga atatgtaaaa aagcagtaca aagagtatac aaaaaagact
      301 gaaaatacaa tgttggattg tagatgtcca gaaagtataa gtcttttaaa aagaaataat
      361 ttgataaata attttgaagt acctatgata gaaccaatat tagtaaggac ttgtagggtt
      421 ttatacgata aatatataaa aagtaaagat gatatgttgg tagtaacctg tccatgtaca
      481 caattaagag actttgcaag tgaaaagttt aaggataagg gtaatgctat gttttataca
      541 tggaaagagt ttgctgaaca agaaggtgta aaatcacttg gaaaaataga tgaatcacca
      601 attccattag gatatttcaa agatactttt gaaaaagttc ttgaagtatc aagtgaagac
      661 gaaatattaa gtgaaataca aaaaataaga aatggttttg aagataagta tgacatagta
      721 gaaatgtttt attgtaaaga tggttgtaat aatggagatg gtttgtaaat ggtcaataaa
      781 tttaataata agtttttaag gctagtaaag agtgttctga tattttttat aattctttta
      841 ttatggaaaa ttacaaatta tcttggtatt tggagtgatt atatattacc atcacctgaa
      901 aaggtatata gtacattttt aaatatgata agtgatggtt caatatttat taatgtatat
      961 gcaagtatga aaagagtttt gataggtttt gcaataagta ctgctatagg tgtgccttta
     1021 gggatattct ttggaattta tagtggagta tacgaatact ttaaatctct aataaatttt
     1081 ttaaggaata cacctcccct tgctttaata ccaatgctta tattatggtt tggtatagga
     1141 gaggaatcaa aaattataat tatagtttta gcatcttttt tcccaatctt tacaagcaca
     1201 ttgaaaggga ttaaaaattg tgactcaaaa ctgattgaag tgggtagagt atttgagttt
     1261 tcaaagcttc agataatatt taaaataata ataccaaatg ccattcttga tattgctgtt
     1321 gggttaaagt tagctcttgg atatagcttt agagcaatta ttggggcaga acttgtagca
     1381 gcttcatctg gtcttggata cttgatatct gatggaaaag aaatgtctag aacagatgtt
     1441 gttattgtag ggataatcgt aattggactt ttaggaataa ttacagatta catattttcg
     1501 attattgtga agaaggtaag taagggaaag atggtggaag catatgaata atttagtatg
     1561 tgactctgga tatataatta gaaatttata taaaagttat aaagttgaca ataaagaaca
     1621 tgtagttctt agtgatatct ctttaaacat agatagaaac catataacag taattttagg
     1681 tgaaagtggt tgtggaaaga ctaccttgct tagagcaatt gcatcacttg aaggtataaa
     1741 tagtggtgaa attaggttta ttaatgataa taaagagtat agacctaatg ttggttttgt
     1801 ttttcaggag agtagattaa tgccttggtt aaacgtaagt gaaaatatag cttttcataa
     1861 tcaaagaaaa aatactattt taaacaaggt gcttagaaaa tttaaacaca ataaaagaat
     1921 taggagtaaa attataaatg ataattttag aagcttagaa acaaatgata atttgaacaa
     1981 atcaaataaa tataatagga aaagtaatat tgatgtagaa aaatatctta agatgatgaa
     2041 cttgcagaat tttaaaaact cttatccaaa tgaattgtca ggaggtatgg cgcagagagt
     2101 aagcatagct agagctcttt ctttcaatcc agatatgttg cttatggacg agcctttttc
     2161 ggcattagat tattttacaa ggattgatat gcaaaatgaa gttataagaa tacataagtc
     2221 aactaataaa ggcgtgatat ttgtaactca tgatattgat gaagcgttaa agataggcaa
     2281 gaaaatagtt gttttcacag acgaaagaaa agtaaaagaa tttaatatag aagatggtta
     2341 taatagagat ttaacttcag attactatat taaattaaaa aaagatattt taatgacaat
     2401 gaaatcaaat aaaggcattt aaagaaatta ataatatttt aaggagtaaa gtaaatgaaa
     2461 ttaaaaaagc tattgatagg tataacgata ttaacacttg caacagcatc tttagctgga
     2521 tgtgctaaaa aaaataatgg agaaaaactt tcagaaataa atttaactta tgtaaaatca
     2581 ccattaaatg ttccttctat aattcagaaa caggatgatt tatttggaaa ggaatttaaa
     2641 aaggataata taaaagttaa cttccacgaa attactacag gtccagagca aacacaagct
     2701 ttagctgctg gagaaataga cttcttacat gcactaggtg gtacatcagc tttaatagct
     2761 gcatctaatg gagtagagtt aaagatatta aacacttata gcaggtcacc aaaaggattt
     2821 atgattttga ctaataataa ttcaataaaa tcagcagcag atttagtagg taagaaggtg
     2881 gcaggcccaa aagggactat attacatcag gtattgattt cagcacttga taaagaagga
     2941 ttaagtatgg atgatgtgga atttgttaat atgggtattc cagaggcatc agcagctctt
     3001 tctgatggca gcgtagatgc tgcattgatt gctggtccag cagctcttaa agctatgaag
     3061 tcaggtagta aacttgtagc aaatggtgaa ggtttagtag atggtataat agtaactgct
     3121 gttagcacag actttgcaga gaaacatcca gaaatagttg aaagatttat gaaggttgaa
     3181 aaggaaactt tagaatatgt aaataataat tttgatgaag ctatggaaaa agtagctaaa
     3241 gaagttgatt taagtcttga agaaactaaa gaattatatg cttggtatga ttttagttta
     3301 gatataacag ataaagacat atcttcgctt gaagatacac aagacttctt aataaaaaat
     3361 aagttacaag agaaaaaagt gaacataaaa gaattaatct ataatgctaa gtagaaacta
     3421 tgataaaata atactaagta agaaatataa taaaagttta agttacctta ttaaggtaac
     3481 tttttctatt ttgaattagt aatttcaatt tattttagaa tgattagttg ataataatga
     3541 ttaattaatc ataaaaagat acttttttat agagttttat taagatgtct aataatatgg
     3601 ttatacttat gaagtcagta attgtgaagt tagtaatatg attaaattta tatattgtaa
     3661 aaagattata atatattaaa attggactgt tatatgatga gtaaataggt atattaggag
     3721 gaattatatg agttataaag tatatattgt ggaagatgat atatcgataa gtacattact
     3781 taaagattat attgagaaat atggatttga agtgattgtt gaagagaact ttgatgatat
     3841 aatgattaca tttgaaagtt ttaagccaca cttagttcta ttagatgtaa atttacctaa
     3901 atttgatggt ttctactggt gtagatgtat taggcaacag tcgaatttac ctataatttt
     3961 tatatcagct agagatggta atgtagacca aattagagct ttagaaagtg gagcagatga
     4021 ttatataaca aaaccttttt actatgatgt agtaatggca aaaataaaga gtcatataag
     4081 aagggtatat ggtgaatatt cacctaaaat agacgaaaga atagtggagc tagatggatt
     4141 aaagttttat ccagaaaggt tagaactaga attaaatggt gagaatctaa ttataactaa
     4201 aaaagaaggt atactgattg aatgtcttat caagagatac ccaaaagtag taagtagaga
     4261 ttatctttta gaaaagatat gggatgatat tgagtttgta gaggaaaata ctttaaatgt
     4321 aaatgttagc aggataagga aaagatttga ggaattaggt atagataatg ctgttcagac
     4381 tgtccgaggg ttaggatata aattaaataa gaattggtaa ggagaaagta tgaagttatt
     4441 tttaaaagat tataaaggtt atatttttat atattatata ggcatagtta tgacaattat
     4501 ttactgtaat cttatgaagt ttattagttt tggagagagt ttatatattc ttttatttag
     4561 ttcttttata ttgttatgtt ttttaatata taagtattat aaaaatagag aagtatataa
     4621 tctgtttgaa aaaggagtac attctttgga ggagtcattt ttgtatttag gaagctcatt
     4681 cttggggaga aatatatcag ttattttaaa acaacaacat aaattgtatc aatcaatgat
     4741 acaagagcat aaaaagatac atagtgaaca tttaactttc atcaaccaat ggattcatca
     4801 gatgaaaaca ccaatatcag ttataagcct tcagcttcaa gattatgagg gagaagaagt
     4861 aactgaaaat ataagaaaag aaatagatag attaaataaa gggttagata tggctatgca
     4921 ttttgcaagg gcagataatt ttcagaaaga ctttatagtt gaaaaagcca aattaaatga
     4981 aattgtatct agtattgtaa atgaagaaaa aaggttattt ataaaaagtg gaatgattcc
     5041 taagattcaa gtagacaaat ttacttatgt ttatacagat gtaaaatgga tgaaatttat
     5101 aataggtcaa ttaattacaa atggagtgaa atattctaaa gattatagta actacctaac
     5161 tataaaatct gaagaaaata ataaatatgt aattttaagt ataatagatg aaggaattgg
     5221 aattgtttct aaagatttaa aaagggtatt tgacccattt tttacaggag aaaatggtcg
     5281 taagtttgga gaatctacag gtatgggtct ttatctagta aaaaaggtat gtgatgattt
     5341 aggtcataaa gtaagtataa aatcagaagt aggaaaaggt acagaagttt caatctactt
     5401 taataaagat gtaaatagtt taataaaggt gtaaattatc tgttacaata gggtttttag
     5461 ttaattgtac ttttttatta tacaatttat taaatgaaat attcatagtt aaataatcaa
     5521 aatttaagag agtttatttg caaaccttac atagatgtaa ggtttgtgta atgtttgcag
     5581 atagttaaat tttctaatct aaattacaat taatataaga aataacataa gagaaataac
     5641 ataaaaaaag aaattttgta aaaaaggaag gatgtttatt gatgagttta gatgttattt
     5701 ttatattgaa atcagtcata atagctatag tagaggggct tacagaattt attccagttt
     5761 catctacagg tcatatgatt ttggttggta atttaataga ttttaagggg caatttgctg
     5821 aaatgtttga ggttgtaata caacttgggg ctatattagc agtagtagta ctttattgga
     5881 aaaagataaa agatagcgtt atagaatttt ttaaatttat atttactggt ggaaaagaag
     5941 gaaagattgg ttttagattt ggaatgaatg taattatagg ttgtatacca tttgcgatta
     6001 taggagttct attttacgat aatataaaat cattgtttaa tttacaatct gtaattatag
     6061 gatttatagt gggtgggatt ttattattag tagtagaaac tttgtttaga aaaaagaatc
     6121 attccacaga taatatagat aaaattactc caatacaagc tttaaaggtt gggacattac
     6181 aagttttgtc tgcatggcca ggtatgtcaa ggagtgcatc tactattatg ggtggatgga
     6241 tagcaggact taattctcct acagcagctg agttttcttt ctttttagca gtaccagcta
     6301 tggtggcttc ttcaggaaaa gatttatttg aatttgatta ttcaataatg acaccaacat
     6361 tgtggattgc tttggtagtt gggtttatag tagcttttat tgtatcaata atagttatgg
     6421 aaaaattcgt gaatttttta aagaaaaaac caatgagagt gtttgctgta tacagaataa
     6481 tcatgggtgt agtacttgca gtattagctt ttactaacat aattagtgta tagaaaaaat
     6541 agatttatta tctataaaaa ttaaactaag ataaattata attaacaatt atatatatgt
     6601 tatttcaaaa attaaattag gaaggatagt taaataatga aaattttaac tgtaaataat
     6661 ttaagtaaag tatatggtaa aaaaataatt ttcaacgcat taaatgacat aaattttagt
     6721 atagaagatg gagaatttgt aggaattatg ggtccatctg gtagtggaaa aactactctt
     6781 ttaaatatga tttctactat tgacaagcca actacaggaa ctatggaatt aaagggcaag
     6841 aatcctttat tgttaagagg tgaggaactt gcattattta gaagaagaga gttaggtttt
     6901 gtatttcagg attttaattt attggataca ttaactatag gagaaaatat agtattgcca
     6961 ttgacattag ataaggtcag tgtaaaagag caagatgaga gattgaatga agtatctaca
     7021 atattaggta taaaagattt attaggaaaa agaacttttg aagtatcagg tggtcaagct
     7081 cagaggactg ctattgctag agcattgatt aacaatccat caatactttt agcagatgaa
     7141 ccaacaggta atttagattc aaagtcatca aaggttgtaa tggagctatt tcaaaagata
     7201 aataaagaaa ataaagttac tactatgatg gtaactcatg acccattagc agcgagctac
     7261 tgtagtagaa tcttattcat aaaagatggg tctatatata atgaaatata taagggtagt
     7321 agtagagagc agttttatca agagataatg gatgtactca cattattggg aggcgataac
     7381 taatgaatat aaaacagttt gctattaaca atatttcaag aaatattaaa gcgtatttag
     7441 gatacttagc aagtattgtt atatcatctt cactgctatt ttcttttatt atgtttacaa
     7501 gtcatccaga tttagatgta tctcaatttc ctgattattt aaaagaaggt ctcaagatga
     7561 gtaagataat tgcatatcta ttcttattct tctttgtgtt ttattcagtt agtgtgtttc
     7621 ttaaaagtag atataaagaa tttggaatac tttatattac aggtatatca aagcgccaag
     7681 taatgaaatt aatctttata gaaaatatga taataaatat agtatcttct gtaattggaa
     7741 taataatagg cttaatcttt gcaaagatat ttttagtggt tatgtctact tttttagaat
     7801 tatcgccatt aaaattctat ataccattta atgcgatatt gtctacttta atctatttta
     7861 tgattttagc tgtattaaca tcaatattta cttcttttat agttaaagaa aatcaggtgt
     7921 tgaagttact taaaggtaca aaaactccaa aaccagagcc taaaacatca ctaatgcttg
     7981 ctatattaag tatttgtttg tttataatag cgtattataa tgctattacc tcaaccgacc
     8041 aatatacaac atatctgcgc ttagttccag ttacatccct tactatagta gcaacttact
     8101 tgttcttttc tcaatttagt gtatttttta tgaagaagct aaaattaaac aagagatttt
     8161 atagaaaaaa tacaaatatg ctttggattt cgaacttgat ttataaggta aaggataatg
     8221 ctagaatatt tttcttgata actataactt ctgcagtggc atttactgct ataggtacag
     8281 tttattcctt ttggaaagat gtagagcgtc aaattaattt aatatatcct aacacaatat
     8341 attattcaac aatgacattg cataatgaca ctaaaaagcc tgatagcaaa gaaaaagaca
     8401 aggaaagaat gatgtttata gaaaataatc taaaaaaaga gaatgtagac tatacaagga
     8461 ttaatggtga atttaaaact gtatttccca agaaaagtga ttttaatgtc agaattatga
     8521 aagaatctaa gtacttagaa attacagaaa ctatagggat taagcctata agttttgaag
     8581 ataatgaatg tatatcatta ttatcatcaa gtttaccagg aaataagaac gtcaaagaaa
     8641 atattattgt agggaataga ggtttaaagg tggcaaaaca agtggaagaa tgtgttatgc
     8701 cagcatatta caaaaatatg tatgtggtaa aggataattt ttatgatagt atagaatcaa
     8761 aatttatagt ggatagattt actgcatttg aagtagagga ttcaagtaaa actatagaga
     8821 tatgcagaga atttgaaaat aaatttaata atgagtcagg gatgcaacca tatttgtttt
     8881 tctctaaaga attaatgata ggaactggga aattaatgta ttctacattt atatttttag
     8941 caatatttat aggtttaatt ttctttgtta ctacaagtag ttttttatat aataaatttt
     9001 atatggattg ccaagttgat aagaaaaagt atgaacagtt aaataaattg ggtatgacat
     9061 ataatgagat taaaaaagct tcaacaattg agattggaat agtatttttg tttccttatg
     9121 tagtggctgt tatacattca atatttgcac ttactgcact taaaaattct tttgatatag
     9181 aagtagcatc atcagctgtt ttggtaatgg gaagcttgtt cttggttcaa atagtttatt
     9241 ttttaattgt aagaaataat tatttaaaag agataaggtt aaatttagtc aatttttagt
     9301 tactattatt aaatgataat aaaaagggtt taaaattaat taatattact ggaattatag
     9361 atgtttattt tttcagtaat attttttatt gtattaacta ttaaaatttt gtacaaatat
     9421 aaaatataat atggtaaatg tcaaaattat aaaaaaattc tacttatata aaatataaag
     9481 aggttatatg tacatatgtg tagaaataaa taataaaaat atcaattaat gttattaggg
     9541 gggggttact cattagtaaa ttagctaata cacttagaat gatgatatta attcaaagca
     9601 gaggcaaaat gaaatgcaaa gaattatcag aagagttgga agtaagtgaa aggcaaataa
     9661 aaagttacaa gatgtattta gagcaagctg gtatatttat aaattcaaca cctgggattt
     9721 atggtggtta tgaaatagat aaatgtaata gcatctcatt aattaaatta ttagatagtg
     9781 aagtttcaat tctagatatg ataaattcac agttagagta taataatgat atttacaaaa
     9841 atgaatttaa taatattgta gaaaaaataa aagcagtatt aaatacagga gaaaaaagcg
     9901 atacatatat ggattatttt acagttcaag cacaacgtaa ttgtgattat gagtctgaaa
     9961 aaaataagtg taatgaaata attagagcat ataccacaaa acacaaattt tggatagaat
    10021 attattcatt aaattcagga aatagtgaaa gaattgttca tccatatggt ttatttaatt
    10081 ataaatcaga tacttatatg gtagcttttt gtgaaaaaag atttaagttt atagacttta
    10141 aattatgtag gattaaagat tataaggttt tagaagaaaa atataatgta gataaaagtt
    10201 ttagttggga tgaatatagt aaaaatagta taggaattta taagggagaa gaaattaatg
    10261 ttgttataaa aattagtcat ccattttcaa caattataaa ggagaaagtt tgggttaata
    10321 atcaacaaat catagaatat gatgataaat ctataatgtt taaagctaaa atgagaggtt
    10381 atgaggagat aaaaagttgg attttaagta tgggagctta tgttgaggtt gtagaaccag
    10441 atagacttag aaatgatata ttgtcagaga ttgaaaaaat gaaaaaaatt tactgatttt
    10501 ttcaaataat ttatacttgg accaatatac ttcacggtct agtgttaata tattaattga
    10561 gagaggtgat attatgaaaa tgaaaattga atttagtaca ggttgtatac caatatctta
    10621 taattcgctc tttatgagta ttataaaaga agcaataaga aaatctaatg aagattacta
    10681 taaaaacatg tactattata aagaaaagaa taataaaaaa actaaaaatt ttacattttc
    10741 ggtttatata aaaaagtata gtattgaagg tgacaatttt attatagaag ataaagtaat
    10801 attaaatata agtactccag atttagaatt gggttttcat atatacaatg ggttaatgac
    10861 ttctaaaaaa tgtttatata aagactatga acttacaaga attagaatcg atttatcaag
    10921 agaaaagaag gtaacagaag aaagggtact atttaatgca ttgtctccaa tctgtgtaaa
    10981 gtctaaagaa ggaaaattct tggagataac tgatgataga tacatagaag aactaaacta
    11041 tataactaat gaagtggtta aaaattatag ggggaatgga ttaaaaagaa agttagaatt
    11101 tgaaaatgta ggtttcaaaa aggtagtagt taaagagagt ttaagagaat ttaaaaaaat
    11161 aacaggtaaa gagtatcaat atattaatgg ttacaaaggt aaatttatat tgaaaggtga
    11221 tattgatgat ttaaatctta tctacaatct aggaattggt tttagacgtg ctcagggatt
    11281 tggagatgta gatattttag aatggaggtg agataaatga agttaagagt ttatagagga
    11341 gattggtttt ttaatatggg tatagttgga tttttaaata taataaagaa agcagataaa
    11401 caagctgaaa tatttattat ggaagattat atcgaatttg atagcttatt tcttgaaaac
    11461 tttcatgagt attattttaa ttactttatg gatgaatatg atgtatcaaa aagaattaaa
    11521 aaaaatattg attacagtat aaattttatt aaatctaagc ctgatagaat caaagatgga
    11581 attaaaaaaa ttaaagatag tgtgaaacaa caaaatgata agattaaaaa atttgatgaa
    11641 gaaaaattta acctgataaa agagaagcta gatagtatgt ctaagataaa aagttatgat
    11701 gaatttgaca gtttagaagc tttagtagat gagactatag atatatttaa aataaaatca
    11761 ataaatgaca ggcttacagc aaatctttat aaatatgtag ttcaagataa ttattttgga
    11821 caagttagtt tttttaatgt tgctaaggcg aaattagatt tagatggatt aaaacaggta
    11881 atgtttaatg attatttaag acaaattata tattttggag agttagaaga tttattagaa
    11941 gagaatgact atgataagtt gaaaaactat ttaaatgata gattaaatag tatagataag
    12001 gatataaatg aaaaaaaagt tagtaagtct agtataaata ctatagaaaa aattatgaag
    12061 gaaataaata aaaactttat aaagaaaaat aaaagcatag aagatataaa agagtatatg
    12121 gatagtttag aagtatgtga aatgtgtggt ttatataagg gaatattaga tgaatactca
    12181 gaaagtaatt ttgctccact tggagtaagc accaataatg ctaggaatat gttttggaat
    12241 caggcgtatg taccatcaat atgtgatatt tgtaagctaa tattgttctg tactccagca
    12301 ggggctactt atacaagaaa gaattattta attaatgaag aaaatgagtt ttatttattt
    12361 gttaacatgg atacttgtat aaatgagctt tttgaaagaa ataatagttt aaaagttcaa
    12421 aaaagtgagt cttcagattc caaagatgaa aatcctttta atcagcttat aaaaagtata
    12481 gtagaagaaa atacattaaa aagtgaatgg caacttcgta atatactttt tgtggaattt
    12541 aaagcaagta tagattctaa aaaatgtaaa ataaattatt ttaatattcc aacttattta
    12601 gcaaagtttt ttacagacaa gtatgcaaat aaaaaaatac aaagtatcta tgattacaag
    12661 cttaaatcaa atgtactaga tttactcttg aaaaataggg acttgaagca tataacgaat
    12721 aacattttaa gaaataaagt taaaaatgat atggaaagca acaacaaatc aaatatatca
    12781 ggaatagatt gttttagagt agtccaatta agagtattaa taaatagcta caaaaagggg
    12841 gtatataaaa tggatagtaa aaatttggag aagaatgatg aaaagctgaa aataatttat
    12901 tatttagggt gtgatataca tgactatttt gttaataaaa attctaaaaa taaaatagat
    12961 ggagtaagct ataaactttt aaattcagtt aaagtcggaa ataaaagtga ttttatggat
    13021 acaattataa gagtttttat gtcagctgaa aaacagattc cagcatttat tttagatata
    13081 gaaattgaaa aagatttaga ctttgaatct ataggtcatg cttttatatc tggattaatt
    13141 tctggaaaat atgaaggaaa agataaacta ccaaacgaaa aggaggaaaa ataagatgaa
    13201 aagagggtta acatttacag taatagcaca agctcaaagt ttaaactatg gtgaagggat
    13261 aggtaatata tcagaattaa aaaaattaag tagagatgat ggaaatatgt atacatttgc
    13321 atctcgccag tgtttaagat atgacatagt acgattagca gcagaattgt ttaattggaa
    13381 tttacaggtt gtcgataaag aaaaaggtac tattcaattt aaagataata taagtataag
    13441 agaatcagaa gaaatggatt tatttggata tatgaaaaca aataaaaaag atgagaaaga
    13501 taaaaaagga ggttctttga ctagagaagc tactgtaaga ttaagtaatg cgatttctct
    13561 tgagccatat agaagtgata tggatttttt aaataataaa ggatttgcag atagaatagg
    13621 agaacaccct aatcttgcta acatagagca acatttaagt tactatacat atacagtgac
    13681 tatagattta tctaaaatag gcaaggatgg agagatagaa cttgataata aagagaaatg
    13741 tagaagagta gtagaatttt tagaaataat aaaagtattg aatagaaata ttagaggtag
    13801 acaagaaaat ttatctcctc tatttgtagt aggaggagta tatgaaatag caaatccatt
    13861 tttcttgggt agaattaaat taaaaggaga taaaaatgga tttaaaataa ataagcaagc
    13921 tatagaagaa gtaattcaag gtactttttt aggaaaagat ttaaaagaat ttacctatgt
    13981 tggaatggta gatggggtat ttataaataa agaagaattt aagggattgt ttgaagataa
    14041 ttttttaagt gtagataaat tttttggaca actagttaaa gaagtaaagg aatattatgg
    14101 ggtgaattaa tatgaaggtt ttgaaattag atttatttca agaaactgca tgctacaaaa
    14161 aaccatttgc tatgaagatt acagagacat atccactgcc tccatattct acaataaatg
    14221 gattaattca caaaatatta aatgctacag aatatatacc atttaatata agtgtacagg
    14281 gaacatatga aagtatattt aataattacc agacaacata tttttataaa aaagatagta
    14341 ttacatctat gccaatgaat agtcatatgc tattgaatgt aaatttgata attcatatag
    14401 gtggggattt tgatttattg gaagaaatat atgatagttt attaaattat gacgagcatt
    14461 tatcattagg tagaaaagaa gatttagtaa gaattaatga tattagattt gtagaagtca
    14521 ataaatttga agtagatgag gatatacaat tagatgatta tgaaaattat aagttatcag
    14581 aatgtaatat aagaatgcct atatatatac caaaaacaag tttacaagat acagatatag
    14641 atggtataag ttacagactc aataattact atgataataa tgctgtggaa gataaaaaaa
    14701 gggtttggaa taaggtagat agttattatg ttgaggatgg aaatactata tcaattggag
    14761 atatattatt agataatgaa ggagatttat tatattttaa taatataaat taggagggag
    14821 attatgttat atgctaaatc taatccagtg gaaacactta gagaacatac tgatgaactt
    14881 ttaaaacaaa tgaatgtatt aagagaaagt tatggaaaaa atataaatag cttagatttt
    14941 ctagaagagg agatattttg gcaattactt gactttgtta tagaatttca tgatattgga
    15001 aaagcatttt caccgtttca agagttaata aaaagcagaa tggatactaa aataaatgaa
    15061 cctaaaatag taactcattt agaaaataat gtaggccata attatctttc tcctgcattt
    15121 attgattata gttatataga caggaaaaaa aataaagaac ttagagcagt attaaatcaa
    15181 gttattgtat atcatcatga aagagatatc tttatagata aagattttaa aaatttaata
    15241 caaaaaatat tagatgaaga tttaataaat aaagtttatg aactacagca tgaatttaaa
    15301 gttagatatc caataaaaac agaaaaattg agtaaagtat atctacaaag tgtagaaaaa
    15361 agaatagata aaaatcacaa gtactataat ttatatatta tgttaaaagg aatactacat
    15421 agattagacc acagtgcttc tgcccatgag attgtagagt gcaatagtaa tatgaatata
    15481 ggcgaataca cagaaaaaca tttagtaaaa gagtttggta gtttaagaga agttcagtca
    15541 tttgctaaat cgaatagaaa caagaatata atactaattg catctacagg gatgggaaaa
    15601 acagaaactg ctttgatttg gatagataag gataaagcat tttttacact accattaaga
    15661 gtaagtataa atgcgttatt tgatagggct aaaaatataa ttggagtagg agaagcaaat
    15721 gatacattct taggtctgtt acattcaaca gcaatagatt atttagaaga aagtaatcag
    15781 gaaaattcaa atgagattgt agatttagca aagttattat cttgtaaact gacattttcc
    15841 acaatagacc aaatatttaa atttcctttt ttgtatagag gctatgaaaa agtgtactca
    15901 acattagcat attcaaaagt agtaatagat gagatacaag catattcacc agaaatagca
    15961 gctgtattgg taaaaggtat agaaatgata cataagatag gtggcagatt tatgataatg
    16021 acagctacta tgccaaccat atatatagat gaattaaaaa aaagaggtgt aatgaatagt
    16081 aacttagctg acttgacatg taacacagaa aaaataagac attgtgtgtc tattgtagaa
    16141 aattcaatag atgaaaactt aggtaaaata atacaaaatg gaatgaataa aaaagttctt
    16201 gtaatagtaa atacagttaa aagtgctgta gaaaaatatg agttaataga agaaatagtt
    16261 aagccaaaag gaatagatgt caacttaaat cttttacatt ctatgtatat acaagaagat
    16321 agagcaaaat tagaaaaata tataaaagaa tttgcagata gtgatagtaa tggtatttgg
    16381 attactacac aattagtgga agcatctctt gatattgatt ttgatgagct tcatacagaa
    16441 aattcaacac ttgacagtct atttcaacga tttggtagat gttatagaag tcgtgagtat
    16501 gaagaaaata gcccaaatat atttatatat acagaaaaag ctacaggaat aggtagtatt
    16561 tatgataagg atattgtaga aaaaggactt gagctactga aaacttttat aaatggaaaa
    16621 gaatgtgtga agatgaaaga aaaatataag gtagaaatgg ttaagatact atactctaaa
    16681 gaaagtctag aaggtacagc ttttgaaaag agatttacca gtgctttaaa tattttagat
    16741 actataacgc catatagttt aggttctaaa gatgcacaaa atatacttag aaatattgaa
    16801 ggatatacag taattccaga ggatatatat aataaaattg aagaaacttt aatcaaagat
    16861 tatgaagagt tgggacaaga gcttataaat gcatatagta atgatgatag acaattgata
    16921 aataaaataa agtctaagag aaaaaaagca agaagagaga taattaaaaa aactgtaaat
    16981 ttaccaatat ataaagctaa acaaaatgta attgacatac aggtgaaagg tcttgaagat
    17041 ttaaaaatac ttttatataa atatgatata tacgaaaata agcatacaaa acagatattt
    17101 ggtaaaggtg tgcttataag taatgaaata gaccaattta tttgattttt atattaacaa
    17161 tgtggctaaa atttactaaa gaaggtgatt ttgtgccaat agatttagaa gtattaaaag
    17221 ttcaaggtgt aaaaataaat tattattatg tatgtaaaag aaaattatgg ctattttcta
    17281 aaggaataac catggaacaa aatagtagta gagtattgtc tgggaagata gtacatgaag
    17341 attcatataa aagaatgaaa aataaagaaa tattgattga tgatatcttg aagctagata
    17401 taataaagga cgagtatgta aaagaagtaa aaataagttc aaagatgaaa gaagcagatg
    17461 aaatgcagtt atactattac ttatactatt taaagaatag tatgggtata gagaaaaaag
    17521 gaactataaa ttatgttaag gaaaagaaac aagaagaatt gatattgaca gaagaaaaag
    17581 aaagaattat tgaaaacaca cttataaata taaatgaaat aaatatgcag ttatctcctc
    17641 ccaaaattga gaaaaaacat tattgtacaa aatgtgctta ttatgaattt tgttttgtga
    17701 aggaggagta acaatgtata aagactatta tattataagt aatggggata taaaaaggaa
    17761 agaaaatacg atttactatt acaatttaga aggtgagaaa aaaatattac cagtagaaca
    17821 agttgatgat attcatatat atggagaagt agatttaaac acaaaactta taaattacat
    17881 aagtaaatat ggggtgatgc ttcatttcta caattactat ggatattatt caggtacata
    17941 ttatccaagg aagaaaaatg tatcaggatt cagtttagta tgtcaatcag cctgctattt
    18001 agataaaaat gaaagattat acttagcaaa atcctttgtg gaaagtgcat cataccatat
    18061 tttaagaaat atgagatatt acaaagttga tgaaagttta attgatacta taaaggatga
    18121 actaaatgat ttgaatgatg tcaaagaaat atcagaactt atgggtaaag aaggcagaat
    18181 aagaaaaaca tactataaat catttaatca aatattaaaa gatgattttg aattttataa
    18241 aagagaaaaa agacctccca aagacccagt aaatgctctt atgtcttttg gaaatagcat
    18301 aatgtatacg aatgttttaa gtgagatata taagacacaa cttgacccta caataagctt
    18361 tttacatgaa ccatcaacaa agagattttc tttaagttta gatattgcag aaatttttaa
    18421 gccaattata attgacacaa taatttttag cctgattaat aataaaagaa ttaataagaa
    18481 tgattttgaa tatcaagaca aaatttgtta tttaagtgaa aaaggaaaga agaaattttt
    18541 gcaagaattt gaaaataaga tgcagacaac tataaaacat agaaatctaa atagaaaagt
    18601 ttcatataga atgtttataa ggttagagtg ttataaatta attaaacatt ttataaatga
    18661 tgaaaggtac aaagttttaa aggtgtggtg gtaagtatgt ttgttattgt tacttatgat
    18721 attgttgaag caaggtcgtt aaatagaatt agaaggatac ttagaaaata tttgacttgg
    18781 acgcaaaatt ctgtttttga aggcaatatt actgaaggaa agttacataa atgtatttct
    18841 gaaatagaaa atattattga taatagcgag gattcaatct atgtttatga gataaaaaat
    18901 cctaattcaa ttaaaaagaa atgttatggg attgataagt attctgatga aatgtttata
    18961 taggtttgca gtgagcgata tttatgctaa aataggtgtt aacagttgga atataaggga
    19021 ttgaaggtgt atgataactg ttatcaattg cactactgct cgctcactgc aaattttgat
    19081 gtttttattg aattataatt gcttgattga agtgttttca atgtattcaa atatacctat
    19141 tttggggttt tatattaact aagtggtatg taaataattt ttgaatcatc tccagtatca
    19201 cttttcttta gggttttata ttaactaagt ggtatgtaaa tcttttagat tttgtagctg
    19261 gtatctcaac aactaccgtt ttatattaac taagtggtat gtaaataata taaactcggg
    19321 tgtttcagat acatctacta tgcaagtttt atattaacta agtggtatgt aaatgcaact
    19381 gtagttggct ggggttataa tgatggaaaa agttttatat taactaagtg gtatgtaaat
    19441 ggaggaccag tggcattttt attacctatg ggtgcacgtt ttatattaac taagtggtat
    19501 gtaaatgagt ttataggaaa gtatggtata caattaagaa aattgtttta tattaactaa
    19561 gtggtatgta aatcttgcta tgtctctaat actaggtgca tatccataca gttttatatt
    19621 aactaagtgg tatgtaaata caaaaacatc ttctccagga actccaatca aatcgcgttt
    19681 tatattaact aagtggtatg taaattaatc tagttttata atctttttta gattgattta
    19741 tgttttatat taactaagtg gtatgtaaat aaggtttgac ctgcgttttt atatgcatct
    19801 gaataacgtt ttatattaac taagtggtat gtaaatcgag ttggcatcca agcatttttc
    19861 acagatttta atagttttat attaactaag tggtatgtaa atacgatatt tttatattct
    19921 attgttgtta tcttattctg ttttatatta actaagtggt atgtaaatag gtatacacct
    19981 tctactcttg taagagtacc ataaggtttt atattaacta agtggtatgt aaattcaaaa
    20041 ttatttagtt atactaaaca ttatattata gttttatatt aactaagtgg tatgtaaatt
    20101 gaaacaaaat gctgatgcaa gaaaagaaag tatccgtgtt ttatattaac taagtggtat
    20161 gtaaatcgaa tttcatggct ttgaatttat ttatatgtag ttgttttata ttaactaagt
    20221 ggtatgtaaa tccaattaag ttactagata aaaaaataga acaaattagt tttatattaa
    20281 ctaagtggta tgtaaataca aaaaaacgta taataaaaat aatttctatc tctggtttta
    20341 tattaactaa gtggtatgta aatcatattc ttcatttatc cctattctat ctaatatttg
    20401 ttttatatta actaagtggt atgtaaattt taaaaggtca acttaaaggt agtaattttt
    20461 taatagtttt atattaacta agtggtatgt aaattatcaa aaaatcccct cacctgcata
    20521 cttgtataaa tgttttatat taactaagtg gtatgtaaat cctgtattct gtttaatgct
    20581 tcaatagctc tttcttgttt tatattaact aagtggtatg taaattatct aaaacccaat
    20641 aggattgaca cgttacgacc ggttttatat taactaagtg gtatgtaaat aggataaaga
    20701 aaagactcac acaagacaca gtgtcaggtt ttatatcaac taagtggtag ataagtagaa
    20761 tattagcaat atcaacgagt atttagaaac ttattttata ttaataaagc agtatataaa
    20821 attgtagaat caacaatagc atatactaaa acatccacct tatattaact aaatcatatc
    20881 taaataaaat acacttacct acaaacatta taaaatcaat acaaaaatga ggtgaaacaa
    20941 aatttatgat aaagaaatta aacagtaaag atataaataa aatcatggaa atatgggaaa
    21001 aaagtacaat caaagcacat gactttataa gtaaagaata ctggcaaaat aactacaata
    21061 ctgttaaaaa cgaatatata cctatatcag atacatttgt atatgatgat ggagatgaaa
    21121 taaaaggatt tataagcata atagataaaa gatttatagg agctttattt ataaagccca
    21181 aataccaaaa tctaggtatc ggaggtaaac ttttagatta tgcaactaaa aagtataaaa
    21241 gtctaagctt agcagtatat aaagataata aaaaagcagt tgtgttttat aataaaaaag
    21301 gttttaatat agtaaaagaa caagtaaatg aagattcagg atttaaagag tacataatgg
    21361 aatatagtaa ataatatgat tacattctat taaaaggata attaatttat ttgccatttg
    21421 attttttaag agtaattttt aaaatagact atatgaagat aaagatattt attataggtg
    21481 taagttaatc agaattgata tagctacaaa agaggaaaat aaaattgtat ggtgtatttc
    21541 agatttaaca aatttactat ttaaagataa taagtttgat ataatattaa agatactttc
    21601 acctataaac tatagggtat ttgaaaga
//
LOCUS       DBGJFF010000062        20813 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.063, whole genome shotgun sequence.
ACCESSION   DBGJFF010000062 DBGJFF010000000
VERSION     DBGJFF010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20813
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.063"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            148..3096
                     /locus_tag="ACOIAF_002275"
     CDS             148..3096
                     /locus_tag="ACOIAF_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HGZ2669284.1"
                     /translation="MRQRIKNLIENEDKKNPLTDEAISSRLNIRREDVTFLRNELKIE
                     DSRQRRKVVLIKAIKDILKEEKTINKNDITRRLNSIGFTVSRFTVIQYLKEIQDSGDL
                     KLDKESDDKKEEIINNRENKDIAFDKLIGSSGSLSTIIKLAKAAILYPPHGLHTIIMG
                     PTGVGKSELAECMYKFAVESSRFPKNAPFIVFNAADYSENPQLLLSQLFGHVKGAFTG
                     ADENKEGLVSKADGGILFIDEIHRLPHEGQEILFQLIDKGMVRKLGETRLTHKIDVMI
                     ISATSEPIDSHLLNTFKRRIPVTIEIPELVARPLNERFDIINNFFLIEAQRMGANIHI
                     KSDVLKALMLYDCIGNVGQLRSDIQVACARGLLNQLTNKLKEVNISISDVPGYVKQGL
                     IKIRNCRGKIENYVDGDLIIDSNLQGEIEKKNEDIYTFPDEIYKLTERRHIDLLEQGL
                     DFDVINRIIGGELESCIQKYIKQVKKVSISNNLPDISPIVGKYIVELTYEVIKIASQY
                     LSSLEPNLHLCLAVHLKAAFERLKEGKVISNAHLKEIIGNYPLEYSVAEKVAQYLKER
                     YNINLPKEEIGIIAIYFKMSSRRDMSEQKKIGVLLMAHGRVASSVCEVTNKLLGINHA
                     RYLDMPFEKKPEEMIEEATTIIKDIDEGRGVIILVDMGSLSYFGDIISGKTGINIRTI
                     SRFDTLLSIEVIRRAVLPDLTIDEIVDEVKGIEPQVKEKVSKCNNTKVDFRKPIILTL
                     CITGLGGACKIKKILEENIKSITEKVDIEPIGMIEYPSLDLEIKQISNDKNVIAIVGT
                     IDPQIANIPFFSLVDINDKAKLNQINNLIDTNQIGETNKKYELSDLIHKEDIFVDLQV
                     KSKEEVLKYMSKKLREKGYVTKNFYKKVVERDNLFPTELSDEIAIPHTDGIDVIRPAI
                     SIAILKKPIVWSKSKVKIILLLAVDQKCFNPLSTLLSFIETDEFKKIKNIKDRDFIRE
                     MILDGVNENN"
     gene            3074..3538
                     /locus_tag="ACOIAF_002276"
     CDS             3074..3538
                     /locus_tag="ACOIAF_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGZ2669285.1"
                     /translation="MESMKIISSNLIFKNIEVSNNEDALKFLGQRLFDEQYVKESYIQ
                     AVVAREKKYATGLPTEIYGVAIPHTDIVHVNEPGIAIGILNKPVKFIMMGTDDTEIDV
                     KVVFMLAVKEPQEQLQLLEKLMTIFQDKNILNNIVDLSEESVSDLLNSKLKN"
     gene            3651..3953
                     /locus_tag="ACOIAF_002277"
     CDS             3651..3953
                     /locus_tag="ACOIAF_002277"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGZ2669286.1"
                     /translation="MGVTNVAKKILVACGTGVCTSTIAINKLKKALQDIGKLDMVNIT
                     QCKVVEVASKAPDYDLIICTTQVSSSIKTPVINGLPFLTGVGMDKLINDVLEELEL"
     gene            4030..5379
                     /locus_tag="ACOIAF_002278"
     CDS             4030..5379
                     /locus_tag="ACOIAF_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HGZ2669287.1"
                     /translation="MEILNFIVGLGAQVMMPIIICIFGLILGTKLGKSLRAGLTVGVG
                     FIGLNTIIALLTDNLGPATQQMVKNLGLSLSIIDVGWPAASAIAFASTVGALIIPIGL
                     VVNIVMLITNTTQTVDVDIWDYWHFAFTGALVAGATQSVMWGVFAAVANMVIVLVMAD
                     LTAPGIEEYLGMPGISLPHGFTQAFVPIAIVVNKLLDLIPGINKIEINADTLQKKFGL
                     FGEPLIMGSVIGVIIGIAAKYDIKGILQLGVTMGAVLILIPKMAALLMEGLLPVSEAA
                     QEFIEKRFKNRGKIYIGLDSAVGIGHPVTLSVALVLVPLTILIAAILPGNKVLPFADL
                     AVIPFALVLIVPITKGNVFRTLIIGIIIITSGLLIATNLAPLFTQMALNASFKMPEGA
                     TMISSICDGANPLSWVFVKVMNYKVIGGVVFGVIALGMAIYNRNRIINENKKLSIEE"
     gene            5414..5887
                     /locus_tag="ACOIAF_002279"
     CDS             5414..5887
                     /locus_tag="ACOIAF_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669288.1"
                     /translation="MKKYKYDSKRYLLSVTIPGFFMILILIYALFNNYTNFNLNIYSL
                     LIIVCTYGIFNTFISSSNPKKIIIDSKCIHFTSFMTTHKYEIKDIEKIRIREFYNKKI
                     YLKINDGKLFKGRYWIRTNMFNNSIALYSYFIELEECLYPDSLKFRNKKFENKNI"
     gene            6059..6709
                     /locus_tag="ACOIAF_002280"
     CDS             6059..6709
                     /locus_tag="ACOIAF_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HGZ2669289.1"
                     /translation="MLLEKLRKEVLQASLDLLNYNLVTLTGGNVSGRDEQTGYIAITP
                     SGMDYRNLTPSDIVIVDVDGNIIDGKWKASVDLSDHLYIYKHREDINSIIHTHSTYSS
                     CFAILNEPIECASTTLANEVGGSVPVAKFSPPTSKKMGKCVIEAIGDKRACLLANHGV
                     IAVGPSVGHALTAAVMLEDSAKVYYLAKSIGTPVLLPDEEIQRARDVFFNVYGQDK"
     gene            6883..7815
                     /locus_tag="ACOIAF_002281"
     CDS             6883..7815
                     /locus_tag="ACOIAF_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HGZ2669290.1"
                     /translation="MYYLLNKSTNPYFNLALEEYLFLNDKYNDDIIIIWRNEESIFIG
                     KNQNPYQEVYHDVIEKGEIPILRRISGGGTVYHDLGNINMSFIQKDRQLHEIDFLEHT
                     KFMQKMLSTLGLDVSITERKDLFLNGKKISGSAQSIKRKNSLYHGTLLYDSDLNKLTK
                     YLNSNKATESNATKSVSSKVTNIKFLLEKDINYFLDYCVEYLKSNIKNIKELELDEED
                     IKSVYNIGEDKYKKIEWTYGKTPKFQVVLPFNNESKINIHVNRWKVSKFFISYDDNVI
                     NMDKLLDLNFFKEEIKESILENYPHYIDLINLIF"
     gene            8089..8802
                     /gene="ispD"
                     /locus_tag="ACOIAF_002282"
     CDS             8089..8802
                     /gene="ispD"
                     /locus_tag="ACOIAF_002282"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HGZ2669291.1"
                     /translation="MYSVIIVAAGSGRRMNLDINKQFIKLREKEIIAHTIQVFYENIN
                     IDEIVVCIKKEEEDFFKENIINKYNFKNIKIAYGGKERQDSIYNGLKKLDKNCDIVLI
                     HDGARPFVDHRIINESIKVAKEKKAVVVGVPVSDTIKIVSDGTVQETPERNLLWAAQT
                     PQTFEYNLIIDAYEQAYKNNYYGTDDSMLVENIGQSVTMVMGSYENIKITSPEDLNIA
                     EQILNMEKRDEVNSRRRII"
     gene            8799..9284
                     /gene="ispF"
                     /locus_tag="ACOIAF_002283"
     CDS             8799..9284
                     /gene="ispF"
                     /locus_tag="ACOIAF_002283"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HGZ2669292.1"
                     /translation="MRIGLGYDVHKLTEDRKLIIGGVEIPHDKGLLGHSDADVLIHAI
                     MDSILGALALGDIGKHFPDTDEEYKGADSMKLLEHVYNLITSKGYKIGNIDSTIIAQS
                     PKMAPYIESMRSNISKVLNTDIDNINIKATTEEGLGFTGAKQGIASQSICLLLLTSQN
                     N"
     misc_binding    9379..9587
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            9651..11366
                     /locus_tag="ACOIAF_002284"
     CDS             9651..11366
                     /locus_tag="ACOIAF_002284"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HGZ2669293.1"
                     /translation="MKMSKMFMPTLKEIPADAEITSHQLMVRSGMIKKMTSGVYNQLP
                     MGLRVFKKIEQIIREELNKKDCQEILCAALLPSELWKESGRWTAMGEEMFRLKDRTER
                     EYCLGPTHEEAFTDIIRQEITSYKQLPLNLYQIQVKYRDERRPRFGVMRTKTFTMKDA
                     YSFDVDDKGLDKSYQDMFDAYVSIFDRCGLENSPVQADSGAIGGSTSAEFMVKSEVGE
                     DEVVFCSGCDYAANVERAESCNLASQKEEMKELEEVHTPGAATIKELEEFLKTSPDKF
                     AKTLVYEADGKTVVVVVRGDREVNEIKVSNAIGSVIEFALATDDVVRKVTNAEVGFAG
                     PIGINADYVFIDKEIVEQRNIVVGANKTEYHIKNANYGRDFEGIVGDFRNVQEGDKCI
                     VCGKPLEIARGVEVGHIFKLGTKYSESMNANFIDKDGKSKPIVMGCYGIGVERTAAAI
                     IEQHNDEKGIIWPLSVAPYHVVIVPANMKNEEQISIAENIYNDLQAMGVEVLLDDRDE
                     RIGVKFNDSELIGIPMRITVGKNINEGKVEFKLRHKEDKEIIDIEEINEKVKAEFIRN
                     NVRLG"
     gene            11488..12933
                     /gene="proS"
                     /locus_tag="ACOIAF_002285"
     CDS             11488..12933
                     /gene="proS"
                     /locus_tag="ACOIAF_002285"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HGZ2669294.1"
                     /translation="MAKNEKQFVEEITKMEDDFPQWYTDVITKTDLVDYAPVKGFMVV
                     KPYGYALWEKMQEFMDKKFKGTGHKNCYFPLLIPESLLNKEAEHVEGFAPEVAWVTHG
                     GNKKLEERLCVRPTSETIICTMYAKWLKSYRELPYLYNQWCSVVRWEKSTRPFLRTSE
                     FLWQEGHTLHETAEEAQEETIQQLEVYKALCEELLAMPVVAGQKSESEKFAGGERTYT
                     IEAMMHDGKALQSGTSHFLGQHFTKAFDITFADREGNLANPYHTSWGASTRLIGGLIM
                     THSDNRGLVLPPRVAPIQVVIVPIAAKKGNVMETVDKIYADLKAKGVAVEVDDRDNYT
                     TGWKFNEWEMKGVPVRVEIGPKDIENNQAMVFRRDTLEKDSMPLEGLADAICDLFDVI
                     HNDMFEKARKHREDNTSIVENMDEFRKALEEKPGFIKTMWCGDAECEAKIKEETGATI
                     RCLPFEQENLGHKCVYCGKEADSMVVMAKAY"
     gene            13371..14852
                     /gene="gltX"
                     /locus_tag="ACOIAF_002286"
     CDS             13371..14852
                     /gene="gltX"
                     /locus_tag="ACOIAF_002286"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HGZ2669295.1"
                     /translation="MNVRVRFAPSPTGFVHIGSLRTALYNYLFAKKMGGEYILRVEDT
                     DQSRLVEGAIENMLNAMKWAGVNHSEGVILDDSGKVVQKGEYGPYIQSQRLDIYQEYI
                     KQLLDSGKAYYCFCTKERLDEVRDAQRAAGETAKYDGHCKNLSKEEVEANIKAGIPYV
                     IRLRLPENHTIKFTDLVRGDMEFNTNDLDDQVLMKTDGFPTYHFAVVVDDYLMKITHV
                     IRGEEWVSSTPKHVYLYEAFGWEAPVFVHLPNILNKEKKKLSKRQGDVAVEDFKKKGY
                     LPEGLVNYVALVGWSPEDNQELFTMEELEKAFSVERVSKSGGVFDTEKLNWVNQHYIK
                     DGDDAYLTDLAIPFLIEDGFITEEEATNKYDFLKSMISVLKEKLQYVKEVTEHASIFF
                     GDKIEVETEEGREFLRLEHIPTLIDALREKIEKTEVLNAEFVQAMLKEIQKEYKIKGK
                     NLFMGSRIILTGQMHGPDLPKVMEVLGKETCLNRIAYVKNNIL"
     gene            15073..15228
                     /locus_tag="ACOIAF_002287"
     CDS             15073..15228
                     /locus_tag="ACOIAF_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669296.1"
                     /translation="MYSNIDDVKKELKELCLEYVTILEKLKDEKMITEETFEKCSSQK
                     KIFLEEQ"
     gene            15373..16770
                     /gene="cysS"
                     /locus_tag="ACOIAF_002288"
     CDS             15373..16770
                     /gene="cysS"
                     /locus_tag="ACOIAF_002288"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HGZ2669297.1"
                     /translation="MKVYNTLTRTKEEFVPLEEGKVKMYVCGPTVYNYIHIGNARPFI
                     IFDTLRRYLEYRGYDVTYVQNFTDVDDKIINRSHEEGISPEEVAAKYIKEYFVDCDGL
                     GIKRATVHPQVTDNIQQIIEFIKELEDKGYAYAVNGDVYFDTNKFEGYGKLSGQKQED
                     LEAGARIEVNDQKRHPMDFVLWKAKKEGEPGWDSPWGEGRPGWHIECSVMSKRYLGET
                     IDIHAGGQDLTFPHHENEIAQSEARSGKTFSKYWMHNGYININDEKMSKSKGNFFTVR
                     DISKLYDLEIVRFFMLSAHYRNPVNFSDEMLNQAKAGLERLYNTKEKLEFTLSNLVES
                     PLTEKEVELVKELDDFRQKFIDAMDDDVNTADAVSVIFELAKLINSNVDENSSLEFAK
                     KCLDEFNELTGVLNIVNKKKDTVLDKDIEELIQKRTDAKKNKEFQLADDIRQQLLDMG
                     IVLEDTRQGVKWKRI"
     gene            16773..17168
                     /locus_tag="ACOIAF_002289"
     CDS             16773..17168
                     /locus_tag="ACOIAF_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III domain-containing protein"
                     /protein_id="HGZ2669298.1"
                     /translation="MEKTELVTMSPLVLAYLGDTVYETYIREYLIRQNTQRKVNDLHK
                     LAIKYVKAKAQATIIHEIEIELTEEESKIYKRGRNQKSNTSPKNADIIDYKHATGFEA
                     LVGYLYLNNEIERLQYIINKGIKIIERDM"
     gene            17274..18032
                     /gene="thyX"
                     /locus_tag="ACOIAF_002290"
     CDS             17274..18032
                     /gene="thyX"
                     /locus_tag="ACOIAF_002290"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HGZ2669299.1"
                     /translation="MKVKLISHTPEPEKVIAMAAKLCYSPVGTDEIEKDLTDESIEKF
                     LNMLLSIGHGSILEHASFTFSIEGISRACSHQIVRHRIASFSQQSQRYVKLEQFEYII
                     PPEIEKIEKAKELFIDSMKKDQENYDKLVEILFENHYNDLIKNGKNEKTAKRQAEKKA
                     IEDARYVFPNACETKMVFTINARSLFNFFEHRCCERAQWEIRNLAVEMLREVKKVAPI
                     LFKKTGPSCVNGSCPEGTMTCGDIVQVREKFKAL"
     gene            18045..18782
                     /gene="rlmB"
                     /locus_tag="ACOIAF_002291"
     CDS             18045..18782
                     /gene="rlmB"
                     /locus_tag="ACOIAF_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HGZ2669300.1"
                     /translation="MASIEGRNPVIEAIKSDREIDKILIANSAKEGSIKKIIGMAKDK
                     NIIIQYVDKHKLDEVSTSHSHQGVIAYASEYKYYELDELIDLAKNKDEDPFFIILDEI
                     TDPHNLGSIIRTADAVGAHGVIIPKRRSVHITPVVAKASAGAVEYMPVCKVTNIVNTI
                     KRLKEEGLWIAAADMDGETFYKQNLTGPLGVVIGSEGFGISRLVKQNCDFIVKMPMIG
                     NVTSLNASVAGGILLYEIFRQRLDKSK"
     gene            18785..19312
                     /locus_tag="ACOIAF_002292"
     CDS             18785..19312
                     /locus_tag="ACOIAF_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HGZ2669301.1"
                     /translation="MKKNINHYLIIDGYNIINAWDNLKELAKEDLEDSREKLIDDIIE
                     FSEFMGYKTIIVFDAYNVKNSREKVEKRKHITIVYTREHQTADSYIEKFITSLSKYDD
                     VKVATNDYAEQQMILGKGATRMSARELKLELDRSKNKMKEKNIGLRKKIQRNWLEERL
                     DKETLSKLENIRRKR"
     gene            19377..20024
                     /gene="sigH"
                     /locus_tag="ACOIAF_002293"
     CDS             19377..20024
                     /gene="sigH"
                     /locus_tag="ACOIAF_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HGZ2669302.1"
                     /translation="MLVAKEKSYELVDNCQQDEYNIVLRASEGDKIALEYIITKYRNF
                     VKAKAKSYFLIGADKEDIIQEGMIGLYKAVRDFDGSKTNSFKCFAEICITRQIITAIK
                     TATRQKHIPLNSYVSLNKPIYDEESDRTLLDIIATSIVTDPEELIISKEELKNIESKM
                     NELLSDLELEVLELYLNGKSYQFIADKLKRDVKSIDNALQRVKRKLEKHLENRND"
     gene            20098..>20813
                     /locus_tag="ACOIAF_002294"
     CDS             20098..>20813
                     /locus_tag="ACOIAF_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006568224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HGZ2669303.1"
                     /translation="MAKAKYERTKPHVNIGTIGHVDHGKTTLTAAITKTLYDRYQLGE
                     AVDFANIDKAPEERERGITISTAHVEYETPNRHYAHVDCPGHADYVKNMITGAAQMDG
                     AILVCSATDGPMPQTREHILLSRQVGVPYIVVFLNKCDMVDDEELLELVEMEVRDLLT
                     EYDFPGDDTPIVRGSALMALEDPKSEWGDKIVELFEQIDEYIPAPERDTDKPFLMPVE
                     DVFSITGRGTVATGRVERGVL"
BASE COUNT         8611 a         2178 c         3909 g         6115 t
ORIGIN      
        1 taaaatataa aaaaaatttt cttaatattt gaataaaaga atagcttaat ttaaatgtta
       61 ttactttata aaaaaataat aagtacaaaa aaaacaatat atggcatata agttgctata
      121 taaataataa ggagggaaca attataaatg aggcaaagaa taaaaaactt aattgaaaat
      181 gaagataaga aaaatccttt aacagatgaa gcgatttcaa gtaggttaaa tattcgaaga
      241 gaagatgtta cctttttgag aaatgagtta aaaatagaag attcaagaca aagaagaaaa
      301 gttgttttaa ttaaagctat aaaagatatt ttaaaagaag aaaaaactat aaataaaaat
      361 gatataacaa gaagattaaa cagcattggg tttacagtat caagatttac agtcatacaa
      421 tacttaaaag agattcaaga ttctggtgac ctaaaacttg ataaagagag tgatgataaa
      481 aaagaagaaa taatcaataa tagagaaaat aaagatatag catttgataa actcataggt
      541 agtagtggta gcttaagtac aattataaaa ctagccaaag cagcaattct ttatccacct
      601 catggtcttc atactattat tatggggcca actggtgttg gaaagagtga gttagcagag
      661 tgtatgtata aatttgcagt agaatcatcc agattcccta agaatgcacc atttatagtt
      721 tttaatgcag cagattattc agagaatcca cagcttttgc tatcacagtt atttggacac
      781 gttaaaggtg cttttacagg agctgatgag aacaaagaag gattagtatc aaaggcagat
      841 ggaggaatat tgtttataga tgaaatacat agattacctc atgaaggtca agaaatacta
      901 tttcaactga ttgataaagg tatggtcaga aagttaggag aaacaagact aacacataaa
      961 atagatgtaa tgattatttc agcaacttct gagccaatag attctcattt acttaataca
     1021 tttaaaagac gtatacctgt aacaattgaa ataccagaat tagttgcaag accattaaat
     1081 gagaggtttg acattataaa taatttcttc ttaatagaag ctcaaagaat gggagcaaac
     1141 atacatataa agtcagatgt attaaaagct cttatgcttt acgattgtat aggtaatgta
     1201 ggtcaactta gaagtgatat tcaagtagct tgtgctaggg gactattaaa ccaacttaca
     1261 aacaaactaa aagaagtaaa tataagcata tcagatgtac ctggatatgt aaaacaaggt
     1321 cttataaaaa tcagaaattg tagaggaaaa atagaaaatt atgtagatgg ggatttaatt
     1381 atagactcaa atcttcaagg tgagatagaa aagaaaaatg aagatatata tacttttcca
     1441 gatgaaatat acaaacttac tgaaagaaga catattgatt tattggaaca aggtttagat
     1501 tttgatgtta taaatagaat cattggagga gaattagaaa gttgcattca aaaatacata
     1561 aaacaagtta aaaaagtttc tattagtaac aatcttccag atataagtcc aattgttgga
     1621 aaatatatcg tagagctaac ttatgaagta atcaaaatag catcacaata cctaagttca
     1681 ctagaaccga atctacattt atgtttagca gttcacctaa aagcagcttt tgagagatta
     1741 aaagaaggaa aagtaatatc aaatgctcat ttgaaagaaa taataggaaa ttatccacta
     1801 gaatattctg tagcagaaaa ggtagctcaa tatttaaagg aaagatataa cataaactta
     1861 ccaaaagaag aaataggtat aatagcaata tattttaaaa tgagtagtag aagagatatg
     1921 tctgaacaaa aaaagatagg tgtacttctt atggctcacg gaagagtagc atcttctgta
     1981 tgtgaggtta caaataaact attaggaatt aatcacgcaa ggtatttaga tatgccattt
     2041 gagaagaaac cggaagaaat gattgaagaa gctactacaa taataaaaga tattgatgaa
     2101 ggtagaggtg tgattatatt agtagatatg ggttcacttt catattttgg agatattatt
     2161 tctggaaaga ctggaattaa tataaggaca atatctagat ttgatacatt gctttctata
     2221 gaggttatac gaagagcagt tttaccagat ttaacaatag acgagatagt tgatgaagtt
     2281 aaaggtatag agccacaagt aaaagaaaag gttagtaaat gtaataatac aaaagtagat
     2341 tttagaaaac ctataatttt aacattatgt attactggat tagggggagc ttgtaagata
     2401 aaaaaaatcc tagaggaaaa tattaaaagt ataactgaaa aggttgatat agaacctata
     2461 ggtatgattg aatatccaag tcttgattta gagataaaac aaataagcaa tgataaaaat
     2521 gtaatcgcca tagttggaac aattgaccct cagatagcaa atatcccttt ttttagtcta
     2581 gtagacataa atgataaagc aaagttaaat caaataaata acctaattga tacaaatcaa
     2641 attggtgaaa caaataaaaa atatgaatta tcagatttaa tacataaaga agatatattt
     2701 gtggatttac aggttaaatc aaaagaagaa gttctaaaat atatgagtaa aaaacttaga
     2761 gagaaaggtt atgtgacaaa aaatttctat aaaaaagtag tggaaagaga taacttattt
     2821 ccaactgaat tatcagatga aatagcaata cctcacacag atggaataga tgtaattagg
     2881 ccagctataa gtatagccat cttaaagaag cctattgtgt ggagtaaaag taaagtaaaa
     2941 ataatacttt tattagctgt agaccaaaag tgttttaatc cattaagcac tctactctca
     3001 tttatagaaa cagatgaatt taagaagata aaaaacatta aagatagaga ttttattagg
     3061 gagatgatat tagatggagt caatgaaaat aattagtagc aatttaattt ttaaaaatat
     3121 tgaagtgtct aacaatgaag atgcattaaa gtttttggga caacgtttgt ttgacgaaca
     3181 gtatgtaaaa gaaagctaca tacaagctgt tgtagcaaga gaaaaaaagt atgctacagg
     3241 gttaccaaca gaaatatacg gagtggcaat accacataca gatatagtac atgtaaatga
     3301 gccaggaata gcaattggaa tactgaataa acctgtaaaa tttataatga tgggtacaga
     3361 cgatactgaa attgatgtaa aagttgtatt tatgttagca gtgaaagagc cacaagaaca
     3421 attgcaacta ttagaaaaac ttatgactat atttcaagat aagaatatac taaataatat
     3481 tgttgactta agtgaagaaa gtgtgagtga tttattaaac tctaaattga aaaattaaac
     3541 atgtaaagta tattaaaaat gtaaaattaa acatgcaaag tatgttaaaa aatataaaaa
     3601 gattattaaa gatttctaac tatagatatc tttaaaataa aataatttca atgggggtga
     3661 ctaatgtggc taaaaagata ttagttgcat gtggaacagg agtatgtact tcaacaattg
     3721 ctataaacaa attaaagaaa gcacttcaag acattggtaa attggatatg gttaatatta
     3781 cacaatgtaa agtagtagaa gttgcatcaa aagcacctga ttatgattta ataatttgta
     3841 ctacacaagt atctagtagt ataaaaacac cagtgataaa tggattacct tttttaactg
     3901 gtgtcggaat ggataaatta atcaatgatg tattagaaga gttagaatta taattaaaaa
     3961 aattaaggaa catggtctct aaaatcataa gttttagaga cttaaaatta aataatggag
     4021 ggtctttata tggagatatt aaatttcatt gttggtctag gtgcacaagt aatgatgcca
     4081 ataataatat gtatttttgg tttgatatta ggtacaaaac taggaaaatc tttaagagct
     4141 ggtttaactg tgggagttgg gtttataggt cttaacacta ttatagcttt gctaacagat
     4201 aatttaggtc cagctacaca acaaatggtt aagaatttag ggctaagttt atcaataata
     4261 gatgtaggat ggccagcagc atcagctata gcttttgcat caacagtagg ggctttaatt
     4321 attccaatag gtcttgtggt aaatatagtt atgctaatta ccaatactac tcaaactgtt
     4381 gatgtagaca tatgggatta ctggcatttt gcttttacag gagctttagt ggcaggggct
     4441 actcaaagtg taatgtgggg agtatttgca gcagtagcaa atatggtaat tgtattggta
     4501 atggcagatt taactgctcc aggtatagag gaatatttag gtatgccagg tatatctctt
     4561 cctcatggat ttacacaggc atttgttcca attgctatag tagtaaataa attattagac
     4621 ttaataccag gcataaataa gattgagata aatgctgata cattgcagaa aaaatttgga
     4681 ttatttggag aacctttaat aatggggtct gtaataggtg taataattgg tattgctgcg
     4741 aaatatgata taaaaggaat attacaatta ggtgtaacta tgggagctgt attaatatta
     4801 attccaaaga tggcagcatt attaatggaa ggattattac cagtatcaga agctgcacag
     4861 gaatttattg agaagagatt taaaaacaga ggcaagattt atataggctt agattcagca
     4921 gtaggaatag gtcatcctgt tacattatca gttgcactag tgttggttcc tttgactata
     4981 cttatagcag ctatattgcc aggaaataaa gttttaccat ttgcagattt agcagtaatt
     5041 ccatttgcac ttgtattaat cgtaccaatt acaaagggaa atgtgtttag aacattaata
     5101 ataggaataa ttataattac ttctggatta ttaatcgcaa ctaacttagc tccattattt
     5161 acccaaatgg ctttaaatgc tagttttaaa atgcctgaag gagcaactat gatttcaagt
     5221 atttgtgatg gtgcgaatcc attaagttgg gtatttgtaa aagttatgaa ctataaagtt
     5281 attggaggag tagtttttgg agttatagct ttaggaatgg ctatttacaa taggaataga
     5341 ataataaatg aaaataaaaa gctatctata gaagaataag gttaaaacaa ttttattaag
     5401 gagtgtatag gttatgaaaa aatataaata tgattctaaa agatacttac tcagtgttac
     5461 aataccagga ttttttatga ttttaatatt gatttatgca ctgtttaata actatacaaa
     5521 ttttaatttg aatatatata gtttgttaat aattgtctgt acttatggta tattcaatac
     5581 ttttatatca tcaagtaatc ctaaaaaaat tattattgat agcaaatgta tacattttac
     5641 ctcatttatg actacacata agtatgaaat taaagatata gaaaagataa gaataagaga
     5701 attttataat aaaaaaattt atctaaaaat taatgatgga aaacttttca aaggtaggta
     5761 ttggataaga acaaatatgt ttaataatag tatagcatta tatagctatt tcattgagtt
     5821 ggaagagtgc ttatatccag attcactaaa atttagaaat aaaaaatttg agaataagaa
     5881 catataaaaa taaaaaattg aattgaagcc tagcttaatc ttttttgata agatataagt
     5941 ttgaacttta tactaaattt ttagaatttt caatcaagac aataacataa tagctaagca
     6001 attagaacac aactaagatg tataaggcat gtatatgatg aatgggagga tttaattaat
     6061 gttattagag aaattaagaa aagaagtatt acaagcatct ttagaccttt taaactataa
     6121 tttagtgaca ttaacaggag gaaatgtaag tgggagagat gaacaaactg gctatatagc
     6181 aatcacccca agtggaatgg attatagaaa tcttacacca agtgatattg ttatagtaga
     6241 tgtagatgga aatattatag atggaaaatg gaaagcatca gtggatttat cagaccactt
     6301 gtatatatat aagcatagag aagacataaa tagtataatt catacacatt ctacatactc
     6361 aagttgtttt gctattttaa atgaacctat agaatgtgca agtacaactc ttgcaaatga
     6421 agtagggggt tcagtaccag ttgcaaaatt ttcgccacca acatctaaaa agatgggaaa
     6481 atgtgttatt gaagcaattg gtgacaaaag agcatgtctt ttagcaaatc atggcgtaat
     6541 tgcagttggt ccatctgtag gacatgctct tacagccgct gtaatgttag aagattcagc
     6601 aaaagtatat tacttagcaa aatcaattgg cacaccagtg ctactgcctg atgaagaaat
     6661 tcaaagagcg agagatgtat tttttaatgt ctatggtcag gataaataaa ttagataaaa
     6721 ttttaaaagt aatatgaaaa tatagatatt caatgatatt aaggagattt ataatgatat
     6781 aataagttta atagttaaga agtaaaatat gaaatgtaat agtatataaa ttcatttaag
     6841 actaaaaaga ataagataaa ataaaaagga gtctgtattt ctatgtatta cttattaaac
     6901 aaaagcacaa atccttactt taatttggca ttagaagaat atctattttt aaatgataaa
     6961 tataatgatg atattataat catatggaga aatgaagaat ccatttttat aggtaaaaat
     7021 caaaatccat atcaggaagt atatcatgat gttattgaaa agggagaaat tcctatttta
     7081 agaagaatat ctggaggagg aactgtttat catgacttag gtaatatcaa tatgtcattc
     7141 atacaaaaag ataggcaact tcatgagata gattttttgg agcatactaa gtttatgcaa
     7201 aagatgcttt caaccttagg actagatgta tctataactg aaagaaaaga tttattttta
     7261 aatgggaaaa aaatttcagg ctctgcacag tctataaaaa gaaaaaattc tctttatcat
     7321 ggaactcttc tttacgattc agatttaaat aaattaacta aatatttaaa ttcaaataaa
     7381 gcaactgaat caaatgcaac taaatctgtt tcaagtaaag ttacaaatat taagttttta
     7441 ctcgaaaaag atataaatta ctttttagat tattgtgttg aatatttaaa atctaatata
     7501 aagaatataa aagaacttga attggatgaa gaagatatta aaagtgtata caatatagga
     7561 gaagataaat ataaaaagat agagtggaca tatggtaaaa cgccgaaatt tcaagtagtg
     7621 ctaccattta ataatgaaag taaaataaat atacatgtta acagatggaa agtatcaaaa
     7681 ttttttattt cttatgatga taatgtaatt aatatggaca agttgttaga tttgaatttc
     7741 tttaaagaag aaataaaaga gagtatttta gaaaattatc cacattacat agatttaatc
     7801 aatttaatat tttaatataa aacctctaat ttgataaaat tgtcttatat caaactagag
     7861 gtttttaata ttcttgcata ttaacaacca ttacaaaatt gtaaaattaa aaacaacact
     7921 attgaaaaat caatatatat tgtaataaat atatgtgttt ttcattagca aatagacatt
     7981 aactatttat acaactgaaa atttaatata taattaatca gatggttaaa ataaaaatat
     8041 taaaagtttt aataaatgat ggttaaaaat aaaatacgag gtgataccat gtatagtgtt
     8101 ataattgttg cagcgggaag cggaaggaga atgaacttag atataaacaa acagttcata
     8161 aaattaagag aaaaagaaat tattgctcat acaattcaag ttttttatga aaatataaat
     8221 atagatgaaa tagtggtatg tataaaaaaa gaagaagaag atttttttaa agaaaatata
     8281 ataaataagt ataactttaa aaatataaaa atagcatatg gcggaaaaga aagacaggac
     8341 tcaatatata atggtttaaa aaaattagat aaaaattgtg atatagtact tatccatgat
     8401 ggagcaagac catttgtcga ccatagaata atcaatgaat ctataaaggt agctaaagaa
     8461 aaaaaggctg tggttgtagg tgttccagta agtgatacta taaagatagt gtcagatgga
     8521 acagtacaag aaactccaga aagaaatttg ctgtgggcag cacaaacccc tcaaaccttt
     8581 gaatataatc tcataataga tgcttatgaa caagcatata agaacaatta ttatggtact
     8641 gatgattcaa tgttggtaga aaatataggt caaagtgtca ctatggtaat gggttcttat
     8701 gaaaacataa aaataactag tccagaagat ttaaatatag cagaacaaat attaaatatg
     8761 gaaaaaagag atgaagttaa cagtagaagg agaattatat gagaatagga ttaggatatg
     8821 atgtacacaa attaacagag gatagaaaac ttataatcgg tggtgtagag ataccacacg
     8881 ataaaggttt gttgggtcat tctgatgctg atgtattgat acatgcaata atggattcta
     8941 ttttaggtgc tctagcatta ggagatatag ggaagcattt cccagataca gatgaagaat
     9001 ataagggagc agatagtatg aaattattag aacatgtgta taatctaata acaagtaaag
     9061 gatataaaat tggtaatata gacagtacta taattgctca aagccctaag atggctccat
     9121 atattgagag tatgagaagt aatatatcaa aagttttaaa cacagacatt gataatataa
     9181 acataaaggc aacaacagaa gaaggtcttg gttttacagg agcaaaacag ggaatagctt
     9241 cacaaagtat ttgtttatta ttgttgacaa gtcagaataa ttaatatatt attagtaata
     9301 agtttaatgg atatcaagta ataggcctat atagaatatc tataatgtat gaatttaagt
     9361 gaaattaata taaatataaa attagctatg ataaagaaat agtaaaatga tgaaatcttc
     9421 acagagagaa aacggtggtg agagttttca agaggaagtc tttgaacctg tcttttagct
     9481 taaaggtata aaaatcttta cggtaggaac cgttatattc ccaaaagaga gctattcata
     9541 tagctaacta gagtggtacc gcggaaatta aacctttcgt ctctatactt gtatagagat
     9601 gagaggtttt ttatattttc aggaagttaa tattcaggag gaatctatta atgaaaatgt
     9661 caaaaatgtt tatgccgaca ttaaaagaaa tcccagcaga tgctgagatt acaagccatc
     9721 aattaatggt taggtcaggc atgataaaaa aaatgacttc aggtgtttat aaccaattac
     9781 caatggggtt aagagtgttc aaaaaaattg aacaaataat tagagaagaa ttaaataaaa
     9841 aagattgtca agaaatactt tgtgcagcct tacttccttc tgaattatgg aaagagtctg
     9901 gaaggtggac tgctatgggt gaagaaatgt ttagactaaa agacagaaca gaaagagagt
     9961 actgtcttgg gccaacacat gaagaagcat tcactgatat aataagacaa gaaattactt
    10021 catataaaca attgccactg aacttgtacc aaatacaagt aaaatataga gacgagagaa
    10081 gaccaagatt tggtgttatg agaactaaga cttttacaat gaaggatgcc tatagttttg
    10141 atgtagatga taaaggttta gataagtcat atcaagatat gtttgatgca tatgtaagta
    10201 tatttgatag atgtggattg gagaatagcc ctgtacaggc agattcagga gctataggag
    10261 gttctacatc agccgaattt atggttaagt cagaagttgg tgaagatgaa gttgtatttt
    10321 gtagtggatg tgattatgca gccaatgtag aaagagcaga atcttgtaat ctagcatcac
    10381 aaaaagaaga aatgaaagaa ttagaagagg ttcatacacc aggagcagct acaataaaag
    10441 agttagaaga atttttaaaa acttctccag ataagtttgc taaaacttta gtatatgagg
    10501 cagatggaaa aacagttgta gtcgttgtaa gaggagatag agaagttaat gaaatcaaag
    10561 tatctaatgc cataggttct gttatagaat ttgctcttgc tacagatgat gtagtgagaa
    10621 aagtaactaa tgcagaagta ggatttgcag gtccaatagg tataaatgct gactatgtat
    10681 ttatagataa agaaattgtt gaacaaagaa acatagttgt tggggctaat aaaactgagt
    10741 accatataaa aaatgctaac tatggaagag attttgaagg aatagttgga gactttagaa
    10801 atgttcaaga aggcgacaag tgtatagtat gtggtaagcc acttgaaata gctagaggtg
    10861 ttgaagtagg acatatattt aaactaggaa ctaagtactc agaatctatg aatgcaaact
    10921 tcatagacaa agatggaaaa tcaaagccta tagtaatggg atgttatgga ataggtgttg
    10981 aaagaacagc agcagctata attgaacaac ataatgatga gaaaggtata atatggcctt
    11041 tgtcagtagc tccataccat gttgtgatag ttccagcaaa tatgaaaaat gaagaacaaa
    11101 tatcaatagc tgaaaatata tataatgatt tgcaagctat gggtgtagaa gttttattag
    11161 atgacagaga tgaaagaata ggtgtcaaat tcaatgattc agaattaata ggaataccta
    11221 tgaggattac agtaggtaaa aatattaatg aagggaaagt agaatttaaa cttagacata
    11281 aagaagataa agagataatt gacatagaag aaataaatga aaaagtaaag gcagagttta
    11341 tcaggaataa tgttaggtta ggataataac ttaaataaga tattgtatag ataatcaaag
    11401 ttgtacttta tagacaccat tttatctata tataaggata taaaatggtg tttttttata
    11461 aaacaaataa atgaggaggt aattgaaatg gcaaaaaatg aaaaacaatt tgttgaagaa
    11521 ataacaaaga tggaagatga ttttccacaa tggtacacag atgtaataac taagactgac
    11581 ttagttgatt atgcaccagt aaaaggtttt atggttgtaa agccatatgg atatgcatta
    11641 tgggaaaaga tgcaagaatt tatggataaa aaatttaaag gaactggaca taaaaactgt
    11701 tattttccat tattaatacc agagagttta ttaaacaaag aagcagaaca cgttgaaggt
    11761 tttgctccag aagttgcatg ggtaacacat ggaggaaata aaaaattaga agaaagatta
    11821 tgtgtaagac ctacttcaga gactataata tgtacaatgt atgcgaaatg gttaaaatca
    11881 tatagagaat taccatatct ttataatcaa tggtgctcag ttgttagatg ggaaaaatca
    11941 actagacctt tcttaagaac atctgagttt ttatggcaag aaggtcatac gttacatgaa
    12001 acagcagaag aagcacaaga agaaacaata caacaattag aagtatataa agcgctatgt
    12061 gaagaattac ttgcaatgcc agttgtagct ggtcaaaaaa gtgaaagtga gaaatttgca
    12121 ggtggtgaaa gaacatatac tatagaagct atgatgcatg atggtaaagc attacaatca
    12181 ggtacaagtc acttcctagg tcaacacttt actaaggctt ttgatattac ttttgcagat
    12241 agggaaggaa atttagctaa tccataccat acatcttggg gagcatctac aagacttata
    12301 ggtggactta taatgactca tagtgataac agaggtttag tattaccacc aagagtagct
    12361 ccaattcaag ttgttatagt tcctatagct gctaaaaaag gaaatgtaat ggagactgtt
    12421 gataaaatat atgctgattt aaaagctaag ggagtagctg tagaagttga tgatagagat
    12481 aactatacaa ctggatggaa atttaatgag tgggaaatga aaggtgttcc agtaagagtt
    12541 gaaattggac caaaagatat agaaaataat caagctatgg tatttagaag agatacttta
    12601 gaaaaggatt ctatgccttt agaaggatta gctgatgcta tatgtgattt atttgatgtt
    12661 atacacaatg atatgtttga aaaagcaaga aaacatagag aagacaatac atctatagtt
    12721 gaaaatatgg atgagtttag aaaagcttta gaagaaaagc caggattcat aaagactatg
    12781 tggtgtggag atgcagaatg tgaagctaag attaaggaag aaacaggagc aactataaga
    12841 tgcttacctt ttgaacaaga aaacttaggt cacaagtgtg tgtactgtgg aaaagaagca
    12901 gatagtatgg tagtaatggc aaaagcttac taaaatacat ttaaataaaa actggggtag
    12961 aaagtattta atactttatc ccagtttttt gttttttatg agtaataaaa tgtatttgta
    13021 actatgtcat aagataagtc atagatactt atattaaaaa gaatataata gagaactatt
    13081 tttaataatt acatattaat gaaagcaaaa tattttgtta tatagatttt caataaagca
    13141 aacaataaat ataaatgaat attagtaagg agtgctttta atttttagaa aataaatgac
    13201 ttaaaagaaa gatttatgaa attaaaacta ggattttctg atataataaa catgtgtatt
    13261 tttaaataaa aaggatatta ttattagatt tgattaaaaa taaacttgca aaacaatgta
    13321 ctagtttaat attatagtta tattgaataa aatatcagga ggaagtaaaa atgaatgtaa
    13381 gagtaaggtt tgcaccaagt ccaacaggat ttgtacatat aggtagtttg agaactgctt
    13441 tatataacta tttatttgca aaaaaaatgg gcggagaata tatattaaga gtggaagata
    13501 cagaccaaag tagattagta gaaggtgcta tagagaatat gcttaatgct atgaaatggg
    13561 ctggtgtcaa ccatagtgaa ggtgttatat tagatgatag tggaaaagta gtacaaaaag
    13621 gagaatatgg tccatatatt caatctcaaa gattagatat ataccaagaa tatataaagc
    13681 aacttttaga tagtggaaaa gcttactact gtttctgtac aaaagaaaga ctggatgaag
    13741 taagagatgc tcaaagagca gcaggagaaa cagctaaata tgatggtcat tgtaagaatc
    13801 tttctaaaga agaagtagaa gcgaatataa aagcaggaat accatatgtt ataagattaa
    13861 gattgccaga gaatcataca attaagttta cagacttagt tagaggagat atggaattta
    13921 atactaatga cttagatgac caagtattaa tgaaaacaga tggatttcca acttatcatt
    13981 ttgcagtagt tgttgatgac tatttgatga aaattacaca tgttataaga ggagaagaat
    14041 gggtatcatc tactccaaaa catgtttatt tatatgaagc ttttggatgg gaagcacctg
    14101 tatttgtgca tttaccaaat atacttaata aagaaaagaa aaaattaagt aaaagacaag
    14161 gtgatgtagc tgttgaagac tttaagaaaa aaggatattt accagaggga ttagtaaact
    14221 atgtagcatt agttggttgg tctcctgaag ataatcaaga attgtttaca atggaggaat
    14281 tagaaaaagc gttctctgta gaaagagtat ctaaaagtgg tggagtattt gatactgaaa
    14341 aattaaattg ggttaatcag cactatataa aagatggtga tgatgcttat ctaacagact
    14401 tagcaatacc atttttaata gaagatggat ttataacaga agaagaagct actaataaat
    14461 atgatttctt aaagagtatg atatcagtac ttaaagagaa attacaatat gtaaaagaag
    14521 ttactgaaca tgcaagtata ttttttggag ataaaataga ggtagaaact gaagagggaa
    14581 gagagttctt aagattagaa catataccta ctttaataga tgctttgaga gaaaaaatag
    14641 aaaaaacaga ggtattaaat gctgagtttg ttcaagctat gcttaaagaa atacaaaaag
    14701 agtacaaaat aaaagggaaa aatttattta tgggctcaag aataattttg acagggcaaa
    14761 tgcatggtcc agatttacca aaagtaatgg aagttttagg aaaagaaact tgcttaaata
    14821 gaatagctta tgtaaaaaat aatatattat agtcataaaa gtccgctaat ttagaagcgg
    14881 actttttttg tgttagtatt tggtgaaaat atctgcttaa gaaaatgaag gtatttaagg
    14941 taataaattt aatatataaa aagcaaagca aaaatataaa taaaatatga ataataaact
    15001 aataaaatat taagcataga agaagggaat ctattatttt agtttgattt aattattaag
    15061 ggggtaatta ctatgtatag taatattgat gatgttaaaa aggaattaaa ggagctatgc
    15121 ttagaatatg ttacaatttt agaaaaatta aaagatgaaa aaatgattac agaagaaact
    15181 tttgaaaagt gctcttctca aaaaaaaata tttttagaag aacaatagag gatggtaata
    15241 gtattattat tatgtagttt gaaagatagt acgaaaaatg atagtaatac tattatttat
    15301 tgcaattttc aactataata taatatataa tgttaagaag ataatttata aaaatacagt
    15361 gaggtgatga gtgtgaaagt ttataataca ttgactagaa caaaagaaga atttgttcca
    15421 ttagaagaag gaaaggtaaa aatgtatgtg tgtggtccaa cagtttataa ctatatacac
    15481 ataggtaatg caagaccatt tataatattt gatactctaa gaaggtattt agagtataga
    15541 ggatatgatg taacttatgt acaaaacttt acagatgttg atgataagat tataaataga
    15601 agtcatgagg agggaatatc tcctgaagaa gttgcggcta agtatataaa agaatatttt
    15661 gtagactgtg atgggctagg aataaaaaga gctactgttc atcctcaggt tacagataat
    15721 atacaacaga ttatagagtt tataaaagag ttggaagata agggatatgc atatgcagta
    15781 aatggtgatg tatattttga tacaaataag tttgaaggtt atggaaaact ttcaggtcaa
    15841 aaacaagaag atttagaggc aggggctaga attgaggtta atgaccaaaa aagacatcct
    15901 atggactttg ttctttggaa ggcaaaaaaa gaaggtgagc caggatggga tagcccttgg
    15961 ggagaaggta gaccaggatg gcatatagaa tgttctgtta tgtctaagag atatttaggt
    16021 gaaacaattg atatacatgc aggtgggcaa gatttaacat tcccacatca tgaaaatgaa
    16081 atagctcaaa gtgaagctag aagtggaaaa acattctcaa aatactggat gcataatgga
    16141 tatataaata taaatgatga aaagatgagt aaatcaaaag gtaatttctt tacagtaaga
    16201 gatatatcaa aattatatga tttagagata gtaagatttt tcatgttatc agcacattat
    16261 agaaatccag taaactttag tgatgagatg ttaaatcaag ctaaagcagg tcttgaaaga
    16321 ctatataata ctaaagaaaa attagaattt acgctaagta atttagtaga atctccgtta
    16381 acagaaaaag aagtagagct tgttaaagaa ttagatgact ttagacaaaa attcatagat
    16441 gctatggatg atgatgttaa tacagcagac gcagtaagtg taatatttga attagctaag
    16501 ttaataaatt ctaatgtaga tgaaaattct tctttggagt ttgctaaaaa atgcttagat
    16561 gaatttaatg aacttacagg agtattaaat attgtaaata agaaaaagga tactgtatta
    16621 gataaagaca ttgaagaatt gatacaaaaa agaactgatg caaagaaaaa taaagagttt
    16681 caattagctg atgatattag acaacaatta ctagatatgg gaattgtatt agaagacaca
    16741 agacaaggtg ttaagtggaa aaggatataa aaatggaaaa aactgaatta gttactatgt
    16801 caccattagt tttagcatac ttaggtgata cagtgtatga gacatatata agagaatatt
    16861 taattagaca gaatacacaa agaaaagtta atgatttgca taaactagct ataaagtatg
    16921 taaaagctaa agctcaggca actataattc atgaaattga aattgagtta actgaagaag
    16981 agagtaaaat ttataaaagg ggaagaaatc aaaagtcaaa tacttctcct aagaatgcag
    17041 atattataga ctacaaacat gcaactgggt ttgaggcatt agttggatat ttgtatttaa
    17101 ataatgaaat agagagactt caatatatta tcaataaagg aatcaaaatt attgaaagag
    17161 atatgtaaat attaaagagt gttgtcttaa taataagtaa taaggcaaca ctttttttat
    17221 aactaatgta taataaatat atagaaatta gtaatgagaa aggataaaaa gttatgaaag
    17281 taaaattaat atcacataca cctgaaccag aaaaagtaat agctatggca gctaagctat
    17341 gttattcacc agttgggaca gacgaaatag aaaaagactt aacagatgaa agtatagaga
    17401 aatttttaaa tatgttatta agtataggtc atgggtcaat attagaacat gcttcattta
    17461 cattttcaat agaaggtatt tcaagggcat gttcacatca aattgttaga catcgtatag
    17521 ctagtttttc acaacaaagt caaagatatg ttaaattaga acaatttgag tacataattc
    17581 ctccagaaat agaaaaaata gaaaaggcaa aagagttatt tatagactcc atgaaaaaag
    17641 accaagagaa ttatgataag ttagttgaaa tattgtttga aaatcattat aatgacctaa
    17701 taaaaaacgg taaaaatgag aaaacagcta aaagacaagc tgagaagaaa gcaatagaag
    17761 atgctagata cgtatttcca aatgcatgtg aaacaaaaat ggtatttact ataaatgcta
    17821 gaagtttgtt taatttcttt gaacatagat gctgtgaaag agcacagtgg gaaataagaa
    17881 atttagctgt ggagatgtta agagaggtta aaaaggttgc tcctatatta tttaaaaaga
    17941 ctggtccaag ttgtgtaaat ggaagttgtc cagaaggaac tatgacttgt ggagatatag
    18001 ttcaagttag agaaaaattt aaggccttgt aggaggtgaa taatttggcg agtatagaag
    18061 gaagaaatcc ggtaatagaa gcaataaaaa gcgacagaga aatagataaa atattaattg
    18121 caaattcagc taaagaaggt tcaattaaga aaataatagg aatggctaaa gataagaata
    18181 taattattca gtatgttgat aaacataagc tagatgaagt aagtacaagt cattcacatc
    18241 aaggtgtaat agcttatgca agtgagtata agtattatga attagatgag ctaatagatt
    18301 tagcaaagaa taaagatgaa gacccatttt tcattatatt agatgagata acagacccac
    18361 ataatttggg ttcaataata aggacagcag atgcagtagg agcacatgga gttatcattc
    18421 caaaaagaag gtctgtacac ataactcctg tagttgccaa agcatctgct ggagcagtag
    18481 aatatatgcc agtttgcaag gtaaccaata tagttaatac aataaaaaga ctcaaagaag
    18541 aagggctgtg gattgctgca gctgatatgg atggagaaac tttttataaa caaaatctta
    18601 ctggtcctct tggtgtagta attggaagtg aaggttttgg aatatctaga ttggttaaac
    18661 aaaactgtga ctttatagta aaaatgccaa tgataggtaa tgttacatct ttaaatgcat
    18721 ctgtagcagg tggaatactt ttatatgaga tattcagaca aagactggat aaaagtaaat
    18781 aggcatgaaa aaaaatataa atcattactt aataatagat ggctacaata ttataaatgc
    18841 gtgggacaat ttgaaagaac tggctaaaga ggacttagaa gattctcgag aaaagcttat
    18901 tgatgacatt atagaatttt ctgagtttat gggttataag actataatag tttttgatgc
    18961 ttataatgtt aaaaattctc gagaaaaagt tgaaaaaaga aaacacataa caatagttta
    19021 tacaagagaa caccaaacag cagatagtta tatagagaaa tttataactt ctttatcaaa
    19081 gtatgatgat gtaaaagtag ccactaatga ctatgcagaa cagcaaatga ttttaggaaa
    19141 aggtgctact agaatgtctg caagagaatt aaagttagag ttagatcgtt ccaagaataa
    19201 aatgaaggaa aaaaatatcg gtttgaggaa aaaaattcag cgaaattggt tggaggaaag
    19261 gttagataaa gaaacattgt cgaaacttga gaacattcgt agaaagcgtt gaaattacta
    19321 ggttctttag actataatat acctataaat ttgttttata tatgggggag atttacatgt
    19381 tggtagcaaa agaaaaaagt tatgagttag tggacaattg tcagcaggat gagtataaca
    19441 ttgtattaag agcaagtgaa ggggataaaa tagcactaga gtacattatt actaaataca
    19501 gaaattttgt aaaggcaaaa gcaaagtcat attttttaat tggagcagat aaagaagata
    19561 taatacaaga aggaatgata ggcctttata aggcagttag agattttgat ggaagtaaga
    19621 ccaattcatt taaatgtttt gcggagatat gtataacaag acaaataata actgctataa
    19681 aaactgcaac aagacaaaaa catatacctt taaattcata tgtttctttg aataaaccaa
    19741 tatatgatga agagtcagac agaacacttt tagatattat agctacaagt atagtaacag
    19801 atccagaaga gcttataatt agtaaagaag agttaaaaaa tatagaatca aaaatgaacg
    19861 aattgttaag tgacttggaa ttagaagtac tagaattata tttaaatgga aagtcatacc
    19921 agtttatagc ggacaagctt aaaagagatg taaagtcaat agataacgct ttacaaaggg
    19981 ttaagagaaa attagaaaaa catcttgaaa acagaaatga ttaatagtaa aatacttacc
    20041 ataagcttat tgtgagcaaa attaatttat atcgaaagat aagggaggaa attcaaaatg
    20101 gctaaagcta aatacgaaag aacaaaacct catgttaata tagggacaat aggacacgta
    20161 gaccacggta aaactacatt aacagcagca ataacaaaaa cattatatga cagatatcaa
    20221 ttaggagaag cagtagattt cgcaaacata gataaagctc cagaagaaag agaaagagga
    20281 atcacaatat caacagcaca cgttgagtat gaaacaccaa atagacacta tgcacacgtt
    20341 gactgcccag ggcatgctga ctacgttaag aacatgataa caggagcagc acaaatggac
    20401 ggagcaatat tagtttgttc agcaacagat ggaccaatgc cacaaacaag agagcatata
    20461 ctattatcaa gacaagttgg agtaccatat atagtagtat tcttaaacaa atgtgacatg
    20521 gtagatgatg aagagttatt agagttagta gagatggaag taagagattt attaacagaa
    20581 tatgatttcc caggagatga cactccaata gtaagaggtt cagcattaat ggcattagaa
    20641 gatccaaaga gtgagtgggg agataagata gtagaattat tcgagcaaat agatgagtat
    20701 ataccagctc cagagagaga tacagataaa ccattcttaa tgccagtaga ggacgtattc
    20761 tcaatcacag gaagaggaac agttgcaaca ggaagagtgg aaagaggagt act
//
LOCUS       DBGJFF010000063        20708 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.064, whole genome shotgun sequence.
ACCESSION   DBGJFF010000063 DBGJFF010000000
VERSION     DBGJFF010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20708
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.064"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            370..681
                     /gene="rpsJ"
                     /locus_tag="ACOIAF_002295"
     CDS             370..681
                     /gene="rpsJ"
                     /locus_tag="ACOIAF_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HGZ2669304.1"
                     /translation="MAKNEKIRIRLKSYDHKLLDFSAGKIVETAKKAGSQVSGPVPLP
                     TEKQVVTILRAVHKYKDSREQFEIRTHKRLIDIANPTPKTVDSLMRLDLPAGVDIEIK
                     L"
     gene            773..1402
                     /gene="rplC"
                     /locus_tag="ACOIAF_002296"
     CDS             773..1402
                     /gene="rplC"
                     /locus_tag="ACOIAF_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HGZ2669305.1"
                     /translation="MKGILGKKVGMTQIFTDKGVVIPVTAVEAGPMVVTQIKTVDKDG
                     YNAIQIGFEDAKEKALNKPKKGHLVAANVLKKHLKEFRVDSVEGYTVGQEIKADVFEA
                     GAKIDVTGISKGKGFQGPIKRHGQSRGPETHGSRYHRRPGSMGACSYPGRVFKNKKLA
                     GHMGSVKVTVQNLEVVKVDADKNLILVKGAIPGAKGSVVTIKEAIKVSK"
     gene            1432..2055
                     /gene="rplD"
                     /locus_tag="ACOIAF_002297"
     CDS             1432..2055
                     /gene="rplD"
                     /locus_tag="ACOIAF_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HGZ2669306.1"
                     /translation="MPKLNVLNVSGQNVGEIELSDSIFGVEVNGHVLYEVVKNQLANK
                     RQGTQSAKTRAEVRGGGRKPWKQKGTGRARQGSTRSVQWVGGGVAFAPKPRSYKYTLP
                     KKVRRLAMKSALSSKVQNSEVIVLDALNMDAPKTKEFAQILNNINAAKKALVVIADKN
                     DNVIKSARNIEGVQTALVNTMNVYDILKYDSFIITTDAVKKVEEVYA"
     gene            2055..2345
                     /gene="rplW"
                     /locus_tag="ACOIAF_002298"
     CDS             2055..2345
                     /gene="rplW"
                     /locus_tag="ACOIAF_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HGZ2669307.1"
                     /translation="MTNPHDVIIRPVVTEHSMAEMGEKKYTFVVAKDANKTEIKKAVE
                     KVFGVSVDKVNTLNYDGKVKRMGRTQGRTSSFKKAVVKLTADSKEIEFFQGM"
     gene            2375..3205
                     /gene="rplB"
                     /locus_tag="ACOIAF_002299"
     CDS             2375..3205
                     /gene="rplB"
                     /locus_tag="ACOIAF_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HGZ2669308.1"
                     /translation="MAIKKFRPTSPALRQMTVLVSDEITCNQPEKSLLVNLKKNAGRN
                     VHGRITVRHRGGGQKRKYRIIDFKRDKDGIPAKVATIEYDPNRTANIALLNYADGEKR
                     YILAPVGINVGDTILSGLGADIKPGNCLALKDMPVGTIIHNIELKPGKGAQLVRSAGV
                     SAQLMAKEGKNALLRLPSGEMRLVSINCKATIGQVGNIEHGNVVIGKAGRKRHMGIRP
                     TVRGSVMNPNDHPHGGGEGRSPIGRPSPVTPWGKPALGYKTRKKNKASNKLIVSRRTK
                     "
     gene            3240..3521
                     /gene="rpsS"
                     /locus_tag="ACOIAF_002300"
     CDS             3240..3521
                     /gene="rpsS"
                     /locus_tag="ACOIAF_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HGZ2669309.1"
                     /translation="MSRSTKKGPFVHARLLKKIEAMNASGNKEVIKTWSRSSTVFPQM
                     VENTIAVHDGRKHVPVYITEDMVGHKLGEFVPTRTFKGHKDDEKSNKRK"
     gene            3553..3888
                     /gene="rplV"
                     /locus_tag="ACOIAF_002301"
     CDS             3553..3888
                     /gene="rplV"
                     /locus_tag="ACOIAF_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HGZ2669310.1"
                     /translation="MEAKATAKYVRVSPRKAGQICDLVRGKNVDEALAILKFTPRGAA
                     SIIAKVVKSAKANAENNHEMDTEKLYIASIVANQGPTMKRFMPRAMGRATTIRKRTSH
                     IEVVVKEKK"
     gene            3911..4726
                     /gene="rpsC"
                     /locus_tag="ACOIAF_002302"
     CDS             3911..4726
                     /gene="rpsC"
                     /locus_tag="ACOIAF_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HGZ2669311.1"
                     /translation="MGQKVNPHGLRVGVIKDWDSRWFATDKKEFGNLLLEDHNIRKFL
                     KKRLYSAGVAKIEIERSANKIKMDLHVAKPGVVIGRAGAGIEALKAELEKMTKKTIIV
                     NIVEVRSTDKNAQLVAENIALAIERRVAFRRAMKQAIQRAMKSGAKGIKVSASGRLGG
                     AEMARTEGYSEGNVPLQTLRADIDYGFAEADTTYGKIGIKVWICNGEVLPTRDGVNPR
                     EESRKSDRRDNKRDNRRNDRRGNDRRGNDNRGNYRGQRPQGGSRPQRTENKGN"
     gene            4762..5193
                     /gene="rplP"
                     /locus_tag="ACOIAF_002303"
     CDS             4762..5193
                     /gene="rplP"
                     /locus_tag="ACOIAF_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HGZ2669312.1"
                     /translation="MLMPKRVKRRRVHRGSMAGQAHKGNKVTYGEFGLVALEASWITS
                     NQIEAARIAMTRYIKRGGKVWIKIFPHKPVTRKPAETRMGAGKGSPEYWVAVVKPGRV
                     MFELAGVSEDKAREAMRLAAHKLPIKCKFVKKEDLEVKGGE"
     gene            5195..5398
                     /gene="rpmC"
                     /locus_tag="ACOIAF_002304"
     CDS             5195..5398
                     /gene="rpmC"
                     /locus_tag="ACOIAF_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HGZ2669313.1"
                     /translation="MKAKELRDLTSEELMNKLNDFKSELFSLRFQLATGQLENTARIK
                     FVKKDIAKVKTVLAERKLYETRA"
     gene            5422..5676
                     /gene="rpsQ"
                     /locus_tag="ACOIAF_002305"
     CDS             5422..5676
                     /gene="rpsQ"
                     /locus_tag="ACOIAF_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HGZ2669314.1"
                     /translation="MERGRRKVRIGRVVSDKMDKTIVVAVEEFVRHPLYNKRVKRTKK
                     FKAHDEKNICNIGDRVKIMETRPLSKDKRFRLVEVVEKVK"
     gene            5702..6070
                     /gene="rplN"
                     /locus_tag="ACOIAF_002306"
     CDS             5702..6070
                     /gene="rplN"
                     /locus_tag="ACOIAF_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HGZ2669315.1"
                     /translation="MIQQESRLRVADNSGAKELLCIRVLGGSKRRYGNIGDVIVATVK
                     SATPGGVVKKGKVVKAVIVRSKQGVRRNDGSYISFDENAAVIIKDDKTPVGTRIFGPV
                     ARELRDNEFMKIVSLAPEVL"
     gene            6094..6399
                     /gene="rplX"
                     /locus_tag="ACOIAF_002307"
     CDS             6094..6399
                     /gene="rplX"
                     /locus_tag="ACOIAF_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HGZ2669316.1"
                     /translation="MRVKKGDTVVVIAGKDKGKKGSVLKVYPKTSKVLVEGVNVITKH
                     QKPSAMNQQGGIINKEAPIHISNVMPFDPETGKGVRVRYEVKDGNKVRVSAKSGKEL"
     gene            6429..6971
                     /gene="rplE"
                     /locus_tag="ACOIAF_002308"
     CDS             6429..6971
                     /gene="rplE"
                     /locus_tag="ACOIAF_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HGZ2669317.1"
                     /translation="MASRLQEKYMKEVAPALMEKFGYKNVMEIPKLNKIVINMGIGDA
                     RENPKGLEKGVEELEMISGQKPVITKARKSVANFKLREGMPIGTKVTLRADKMFYFMD
                     KLVSVSLPRVRDFRGVNPNAFDGRGNYALGVKEQLIFPEIEYDKIDKVRGMDIIFVTT
                     AKTDEEARELLKLLGMPFSK"
     gene            6989..7174
                     /locus_tag="ACOIAF_002309"
     CDS             6989..7174
                     /locus_tag="ACOIAF_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HGZ2669318.1"
                     /translation="MARKAMVVKQQRKQKYATREYTRCTICGRPHSVLKKFGICRICF
                     RELAYKGQIPGVRKASW"
     gene            7207..7605
                     /gene="rpsH"
                     /locus_tag="ACOIAF_002310"
     CDS             7207..7605
                     /gene="rpsH"
                     /locus_tag="ACOIAF_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003241998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HGZ2669319.1"
                     /translation="MTMTDPIADMLTRIRNANVVKHETVDVPASNMKKELARILLEEG
                     FIRGYDVIEDGKQGIIRIQLKYGQEGERVITGLKKISKPGMRVYAANHEIPKVLNGLG
                     ISVISTSKGILTDKQARKENVGGEVICYVW"
     gene            7636..8178
                     /gene="rplF"
                     /locus_tag="ACOIAF_002311"
     CDS             7636..8178
                     /gene="rplF"
                     /locus_tag="ACOIAF_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HGZ2669320.1"
                     /translation="MSRIGVKPIIIPAGVEVTIAEGNLVTVKGPKGTLTKQLSAELNI
                     KKEENTIMVERPTDNKKHRSLHGLTRTLLDNMVVGVNTGFEKKLELKGVGYRAQKQGK
                     KLVMNLGFSHPVEMEDPEGITVEAPNQTELIVKGIDKQLVGNYAAKIRAWRKPEPYKG
                     KGIKYVDEVIRRKEGKTGKK"
     gene            8214..8582
                     /gene="rplR"
                     /locus_tag="ACOIAF_002312"
     CDS             8214..8582
                     /gene="rplR"
                     /locus_tag="ACOIAF_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HGZ2669321.1"
                     /translation="MLKKADKNANRLQRHKRVRRKISGTSQRPRLCVFRSANNIYAQI
                     IDDTKRVTLVAASSLEAEVKSAVNHTGNKEAAKKVGELVAKKAVEKGITEVVFDRGGY
                     LYHGRIQELAEGAREAGLKF"
     gene            8603..9112
                     /gene="rpsE"
                     /locus_tag="ACOIAF_002313"
     CDS             8603..9112
                     /gene="rpsE"
                     /locus_tag="ACOIAF_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HGZ2669322.1"
                     /translation="MLRRKPIDAGQLDLQEKVVEVRRVTKVVKGGRNFRFAALVVVGD
                     ENGHVGIGAGKAMEVPDAIKKAVEDAKKNLIVVPIVGTTIPHEVRGHFGAGNILIMPA
                     VEGTGVIAGGPARAVLELAGLKDVRAKSLGSNNPRNMVNATIEGLNSLKTVEDIAKLR
                     GKKVEELLG"
     gene            9127..9312
                     /gene="rpmD"
                     /locus_tag="ACOIAF_002314"
     CDS             9127..9312
                     /gene="rpmD"
                     /locus_tag="ACOIAF_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HGZ2669323.1"
                     /translation="MAKLQIKLVRSVIGTTPNQKKNVEALGLRKREQVVVKEDNAQTR
                     GMINKVSHLLEVTEIAE"
     gene            9345..9788
                     /gene="rplO"
                     /locus_tag="ACOIAF_002315"
     CDS             9345..9788
                     /gene="rplO"
                     /locus_tag="ACOIAF_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HGZ2669324.1"
                     /translation="MKLHELKPAEGAVRAKRRLGRGTATGQGKTAGRGQKGQWSRSGG
                     GVRVGFEGGQMPLARRLPKRGFNNIFKKVYTEVNVEVLNRFENGTEITAELLKSTKTI
                     SKIGKDGIKILGEGNLEKALTVKAAKFTASAQEKIEKAGGKAELV"
     gene            9832..11100
                     /gene="secY"
                     /locus_tag="ACOIAF_002316"
     CDS             9832..11100
                     /gene="secY"
                     /locus_tag="ACOIAF_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HGZ2669325.1"
                     /translation="MLSKLKQAWKIKAVRKKVMYTLMMIVIFRIGTTIPVPGIDTSII
                     QKMVGGNSLLSLYNMFTGGAFSNFSLFALGISPYITASIIIQLLTVGFESLAELQKSG
                     EEGKKKINKYTKYTALALAVVQALGITLGIVRSALISNSVFFITTVVITLVSASMLVM
                     WIGDKITEKGIGNGSSVIIFAGIISRIPTDVIKISQQVKSGEVAPWVIVILAVVILLT
                     VTGVTFIQEATRKIPVQYAKRVVGRKMYGGQSSHIPMKVNQSGVMPIIFASSLLAFPQ
                     TIAMFMGPNAQAFVQKYLSMATEQGFWTYRSIEILLIIFFSYFYTTVSFNTEDISKNM
                     KNNGGFIPGIRPGEPTMDYLNRILTRLTLAGATFLAIIAMVPALTTHYMKVNMSLAGT
                     SLLIVVGVALELKRQLESNLVMRSYQGFLK"
     gene            11117..11767
                     /locus_tag="ACOIAF_002317"
     CDS             11117..11767
                     /locus_tag="ACOIAF_002317"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HGZ2669326.1"
                     /translation="MRIILLGPPGAGKGTQAAGIVEKYNIPHISTGDIFRKNIKEGTE
                     LGKKAKEYMDQGLLVPDELTVGLVTDRISQEDCKNGFMLDGFPRNVAQGEHLDIFLKN
                     AGISLDKVVNIEVDKSILVSRAVGRRICKSCGATYHVEFNPPKVEGVCDVCQGELYQR
                     ADDNEETVSKRIQVYLDETKPLVDYYSKQGIIADIKGDQAIDKVFEDIVAALGSGK"
     gene            11768..12514
                     /gene="map"
                     /locus_tag="ACOIAF_002318"
     CDS             11768..12514
                     /gene="map"
                     /locus_tag="ACOIAF_002318"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HGZ2669327.1"
                     /translation="MIILKSKKEIELLREAGKIVADTHEVLRKAISPGISTLELDKIA
                     EENIRKYNAEPSFKGYGGFPGSICASINREVVHGIPGETILQEGDIVSLDIGAYYKGY
                     HGDSAKTHGVGMISEEDRKLIEVTRESFYEGIKFAKLGYRLSDISHAVQTHVEKHGFS
                     VVRDLVGHGVGANLHEDPQVPNYGLPGKGPRLREGMVIAIEPMVNAGRYHVKTLSDGW
                     TTVTIDGKKSAHYEHTIAITEHEPLILTKL"
     gene            12536..12805
                     /locus_tag="ACOIAF_002319"
     CDS             12536..12805
                     /locus_tag="ACOIAF_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KOW domain-containing protein"
                     /protein_id="HGZ2669328.1"
                     /translation="MLSDNLSIGQVVKVSLGRDKGNLFFVVKIINNEYVLIADGKKRK
                     LDKPKLKKVKHLKKYNFINDEVRKRVVSGQEITDSFLRAELTKLN"
     gene            12843..13061
                     /gene="infA"
                     /locus_tag="ACOIAF_002320"
     CDS             12843..13061
                     /gene="infA"
                     /locus_tag="ACOIAF_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HGZ2669329.1"
                     /translation="MAKKDVIELEGTVSEALPNAMFKVKLENGHEILCHISGKLRMNF
                     IRILEGDKVNVELSPYDLTRGRITWRKK"
     gene            13097..13210
                     /gene="rpmJ"
                     /locus_tag="ACOIAF_002321"
     CDS             13097..13210
                     /gene="rpmJ"
                     /locus_tag="ACOIAF_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HGZ2669330.1"
                     /translation="MKVRPSVKPICEKCKVIKRKGKVMVICENPKHKQKQG"
     gene            13363..13734
                     /gene="rpsM"
                     /locus_tag="ACOIAF_002322"
     CDS             13363..13734
                     /gene="rpsM"
                     /locus_tag="ACOIAF_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HGZ2669331.1"
                     /translation="MARIAGVDLPREKRAEIGLTYIYGIGKATANEILAKAEINPDTR
                     IKDLSEDQVNELRKVIDDDFLVEGDLRREIALNIKRLRDIKCYRGIRHAKGLPLRGQR
                     TKTNARTRKGPRKTVSRKKKK"
     gene            13770..14168
                     /gene="rpsK"
                     /locus_tag="ACOIAF_002323"
     CDS             13770..14168
                     /gene="rpsK"
                     /locus_tag="ACOIAF_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020449874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HGZ2669332.1"
                     /translation="MAKPKKKVTRIRRRERKNIERGHAHIQSTFNNTIITLTDVHGNA
                     ISWASSGQLGFKGSRKSTPFASQMAAETAAKAAMEHGLKSVEVFVKGPGSGREAAIRA
                     LQATGLEVTMIKDVTPIPHNGCRPPKRRRV"
     gene            14200..14823
                     /gene="rpsD"
                     /locus_tag="ACOIAF_002324"
     CDS             14200..14823
                     /gene="rpsD"
                     /locus_tag="ACOIAF_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HGZ2669333.1"
                     /translation="MARYTGASCRQCRREGMKLFLKGDRCYTDKCAIVKRNYAPGQHG
                     QGRKKVSNYGLQLREKQKVKRIYGVLETQFRNLYERAENMPGKAGENLLSLLERRLDN
                     VVYRMGLASSRKEARQLVTHGHFTLNGNKVDIPSLIVKVGDVIEVKEKSRSSAKFKNL
                     VEVNSRIAPKWLEANVEGMTAKVVGVPTREDIDLEIAEHLIIELYSK"
     gene            14901..15848
                     /locus_tag="ACOIAF_002325"
     CDS             14901..15848
                     /locus_tag="ACOIAF_002325"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HGZ2669334.1"
                     /translation="MIEIEKPKVDIVELSEDYRYGKFVIEPLERGYGITIGNALRRIL
                     LSSLPGVAVNAIKIDGVLHEFSTIPGVKEDVTEIILTLKELSATIDGEGSRTLKIEAQ
                     GPCSITGADIICPPDVEILSKDLAIATLDDNAKLNMEIFVDKGRGYVSAEENKTENVP
                     IGVLPVDSIYTPVEKVSYHVENTRVGQKTDYDKLVLEVWTNGSINPQEGISLAAKVLV
                     EHLNLFIDLTEHVSSVEIMVEKEEDQKEKVLEMTIEELDLSVRSYNCLKRAGINTVEE
                     LANKSEDDMMKVRNLGKKSLEEVIQKLEELGLGLKPSEE"
     gene            15869..16210
                     /gene="rplQ"
                     /locus_tag="ACOIAF_002326"
     CDS             15869..16210
                     /gene="rplQ"
                     /locus_tag="ACOIAF_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HGZ2669335.1"
                     /translation="MAKYRKLGRETAHRNLMLRNLVTCLLRSGRIETTVTRAKETRRM
                     AEKMITLAKRGDLHARRQVLAYVMDETVVNNLFTDLAPKYAERNGGYTRIIKIGPRKG
                     DAAEMAFIELV"
     gene            16330..17163
                     /locus_tag="ACOIAF_002327"
     CDS             16330..17163
                     /locus_tag="ACOIAF_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HGZ2669336.1"
                     /translation="MDNIVKVNNISFEYITDEAKLKAIDNLSLDVKKGEFVAIIGHNG
                     SGKSTLSKNLNAILMPTEGNILIDDMDTKEEERLWDIRQTAGMVFQNPDNQIVATIVE
                     EDVAFGPENLGIEPKEIRRIVEESLKSVGMYDLRDRQPHLLSGGQKQRVAIAGIIAMR
                     PKCIIFDEATAMLDPSGRKEVMKTIKRLNKEENITVIHITHFMEEAVEADRVVVMEKG
                     KKILEGTPREVFSKIKMLKEIGLDVPCMTELSSLLIEEGINISSDILTVDEMVMELCQ
                     L"
     gene            17151..18017
                     /locus_tag="ACOIAF_002328"
     CDS             17151..18017
                     /locus_tag="ACOIAF_002328"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HGZ2669337.1"
                     /translation="MSIIVKNLTHIYNEGMPFASKALDDVSFEIKDRDFVGLIGHTGS
                     GKSTLIQHLNGLLKPSSGEIFINDFNITDKNLNLTEIRKRVGVVFQYPEYQLFEETID
                     KDIAFGPSNLGLEESEIHNRVKASMEAVGLDYEGFKDKSPFELSGGQKRRVAIAGVIA
                     MNPEVLILDEPTAGLDPGGRDEIFNLIKDLHEKKNMTIILSSHSMDDMAKLAKTLIVM
                     NHGSVEFMGTPREVFKSNASKLKDIGLDIPQVLELALKLREKGFDISEDILTLEEAKQ
                     EILKVVRGRGLC"
     gene            18011..18814
                     /locus_tag="ACOIAF_002329"
     CDS             18011..18814
                     /locus_tag="ACOIAF_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HGZ2669338.1"
                     /translation="MLKDITIGQYYPTSSAIHKLDPRIKLVATIVFMVSIFVVNKFWP
                     YIVVLLCLLAMIKLANIPVKYIVKGVKPLKWIILFTFLINIFFLPGDEIWSFGFLAIT
                     KQGLRQAIFMAIRLIFLVVGTSLLTLTTSPIELTDGIERLLNPFKKIGLPVHELAMMM
                     TIALRFIPTLLDETDKIMKAQMSRGADFESKNLINRAKNLVPLLVPLFVSAFRRADEL
                     AMAMEARCYRGGHNRTKMRESVISRRDYMACVFQVVYLGAIIATRFIAI"
     gene            18846..19577
                     /gene="truA"
                     /locus_tag="ACOIAF_002330"
     CDS             18846..19577
                     /gene="truA"
                     /locus_tag="ACOIAF_002330"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HGZ2669339.1"
                     /translation="MRNIKIKIQYNGKNYCGWQKQPDSLGIQGTIERAIYDITKEETS
                     LIGSGRTDSGVHAIGQIANFKINSGISIESIPMALNAKLPKDISVIEACEVNDDFHSR
                     YSAKGKTYKYLVYNSKFRNPILSEISYQVKYELDFDKMCSEAKSLLGTHDFKGFMSSG
                     SSVKDTVRTIYDIDISKKDDLITFEISGNGFLYNMVRIIVGTLVDMGRGRINEPFLDI
                     IQSKTRSRCGHTAPAQGLFLKKVHY"
     gene            19697..20128
                     /gene="rplM"
                     /locus_tag="ACOIAF_002331"
     CDS             19697..20128
                     /gene="rplM"
                     /locus_tag="ACOIAF_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HGZ2669340.1"
                     /translation="MKSYIAKPADVQRKWYLVDAEGKTLGRLATEIATVLRGKHKPTF
                     TPHVDGGDFVVVVNAEKIVLSGKKLDQKYYRYHTGYVGGLKEISYRDMMDKKPEEVIS
                     HAVSGMLPKNKLRSRMMTRLRVFAGAEHTHAAQNPEVLNFK"
     gene            20157..20549
                     /gene="rpsI"
                     /locus_tag="ACOIAF_002332"
     CDS             20157..20549
                     /gene="rpsI"
                     /locus_tag="ACOIAF_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HGZ2669341.1"
                     /translation="MANVQYYGTGRRKSSVARVRLVAGEGNILVNGRALENYFNYETL
                     IRDVKQPLVLTGNENKYDVIVKVEGGGFTGQAGAIRHGISRALLKADLDLRPALKKEG
                     FLTRDARMKERKKYGLKAARRAPQFSKR"
BASE COUNT         8266 a         2444 c         4322 g         5676 t
ORIGIN      
        1 ctttactaaa ctaatggatt taatataaaa aaagaggggg gtaactcctc tcttttttgt
       61 tttttgttga caagtagcgc taaagcatat atactaaact agtgccttga gacagattaa
      121 caatcaagtt taaggttatg atgttgtaat agaaaataaa attgaaaaaa acagaaaaaa
      181 aacttgaaaa aatgtaacag atgatataaa atataatagt gtgcttgaga gattaagaaa
      241 ataataatta aatcctagtg aaatacgaac tgatatcgat ttaaaagtta caacacaccc
      301 ggagcagtgt atcggtattg gttgtacgca acttgaaaat taaacacgtg ataaaggagg
      361 gaaaacaaaa tggctaagaa tgaaaaaata agaataagat taaaatcata tgatcacaaa
      421 ttattagatt tttcagcagg taaaatagtt gaaactgcta aaaaggctgg atcacaagtt
      481 tcaggacctg tgcctctacc aacagaaaaa caagttgtaa ctatattaag agctgtacat
      541 aagtacaaag actctagaga acaatttgaa ataagaactc ataaaagact aatcgacata
      601 gctaatccaa cacctaagac agttgactca ttaatgagat tagacttacc agcaggtgta
      661 gatatagaaa taaaattata agaaccccta agatattatc ctatagatga ttatcttaga
      721 atgattactc taaaagatgt aatccgctgt aagaattata ggaggtgcta atatgaaagg
      781 aatattaggc aaaaaagttg gtatgactca aatattcact gataaaggtg tagttatacc
      841 tgtaacagct gttgaagcag ggcctatggt cgttactcaa ataaaaacag ttgataaaga
      901 tggatacaat gctatacaaa taggttttga agatgctaaa gaaaaagctt taaacaaacc
      961 taaaaaagga catttagttg ctgctaatgt tttaaagaaa catttaaaag aatttagagt
     1021 agattctgta gaaggataca cagtaggaca agaaataaaa gctgatgtat ttgaagcagg
     1081 tgcaaaaata gatgttactg gaataagtaa aggtaaggga ttccaaggtc caataaaaag
     1141 acatggacaa agtagaggtc ctgaaactca cggttctaga taccacagaa gaccaggttc
     1201 aatgggagct tgttcttacc caggtagagt atttaaaaac aaaaaattag ctggacatat
     1261 gggaagcgta aaggtaacag ttcaaaactt agaggttgtt aaagtagatg ctgacaagaa
     1321 ccttatatta gttaaaggag ctataccagg agctaaaggt tcagtagtaa ctataaaaga
     1381 agctataaag gtttctaaat aatgactaac ctagagaaag gaggaataac aatgccaaaa
     1441 ttaaatgtac taaatgttag tggacaaaac gttggagaaa tagaattatc agattctata
     1501 tttggcgtag aagttaatgg acatgtttta tacgaagttg taaaaaatca attagcaaat
     1561 aagagacaag gaactcaatc tgctaaaact agagcagaag ttagaggtgg cggaagaaaa
     1621 ccttggaaac aaaaaggaac aggtagagca agacaaggtt ctacaagatc tgtacaatgg
     1681 gtaggtggag gagttgcttt tgcacctaaa ccaagaagct acaaatatac attacctaag
     1741 aaggtaagaa gattagctat gaagagtgct ttatcttcaa aagttcaaaa tagtgaagtt
     1801 atagtattag atgcattaaa catggatgct cctaagacta aagaatttgc tcaaatatta
     1861 aacaatataa atgctgctaa aaaagcttta gtagttatag ctgacaaaaa cgataacgta
     1921 ataaaatcag ctagaaatat agaaggtgtt caaactgcat tagttaatac tatgaatgta
     1981 tatgatatat taaaatacga ttcatttata ataactacag atgctgttaa aaaagtggag
     2041 gaggtgtacg cataatgact aatccacatg atgtaataat aagaccagtt gtaactgagc
     2101 acagtatggc tgaaatgggt gaaaaaaaat acacttttgt tgtggctaaa gatgcgaaca
     2161 aaactgaaat aaaaaaagca gtagaaaaag tattcggagt tagtgttgat aaagtaaata
     2221 ctttaaacta cgatggaaaa gttaaaagaa tgggtagaac tcaaggaaga acatcaagct
     2281 ttaagaaagc agtagttaaa ttaactgctg atagtaaaga aatagaattc ttccaaggaa
     2341 tgtagtatta taacaaacga aggagggaaa acagatggct ataaaaaagt ttagaccaac
     2401 ttctcctgcc ttaagacaaa tgacagtttt agtttctgat gaaataacat gtaatcaacc
     2461 agaaaaatct cttttagtta acttaaagaa aaatgctggt agaaacgtac atggtagaat
     2521 aactgttcgt catagaggtg gaggacaaaa aagaaaatat agaataatag attttaaaag
     2581 agataaagat ggtatacctg ctaaggttgc tactatagaa tatgatccaa acagaactgc
     2641 aaatatcgca ttattaaact atgctgatgg agaaaaaaga tatatattag ctccagttgg
     2701 aattaatgtt ggagatacta tattatcagg attaggtgct gatataaaac caggaaactg
     2761 tttagcatta aaagatatgc cagttggtac tatcattcat aatatagaat taaagccagg
     2821 aaaaggagct caattagtta gatctgctgg agtttctgca caattaatgg ctaaagaagg
     2881 aaaaaatgct ttattaagat taccatcagg tgagatgaga ttagtaagca taaactgtaa
     2941 agctactata ggtcaagttg gaaatataga gcatggtaac gtagttatcg gtaaagcagg
     3001 tagaaaaaga catatgggaa taagacctac tgtaagaggt tctgtaatga accctaatga
     3061 ccatccacac ggtggagggg aaggtagatc tccaataggt agaccatctc ctgttactcc
     3121 atggggtaaa ccagctcttg gatacaaaac tagaaagaaa aataaagctt caaataaact
     3181 aatagtatca agaagaacta agtagtaaat ttatttttgc tcggaaagga ggaatataaa
     3241 tgtcaagatc aactaaaaaa gggccttttg tccatgcaag acttttaaag aagatagaag
     3301 ctatgaatgc tagtggaaat aaagaagtta taaaaacttg gtcaagatct tcaactgtat
     3361 ttccacaaat ggtagaaaac actatagctg ttcatgatgg aagaaaacat gttccagtat
     3421 atataacaga agatatggtt ggacataaat taggtgagtt cgttcctact agaactttca
     3481 aaggacataa ggatgacgaa aaatctaata agagaaaata attaaattat gactaagaaa
     3541 ggaggaataa caatggaagc aaaagctact gctaaatacg tacgtgtatc accaagaaaa
     3601 gcaggccaaa tatgtgacct tgttagagga aagaatgttg atgaagcatt agcaatatta
     3661 aagtttactc caagaggagc agcttcaata atagctaagg ttgtaaaatc agctaaagct
     3721 aacgcagaaa acaatcacga aatggatact gaaaaattat atatagcatc aatagttgct
     3781 aatcaaggac caacaatgaa gagattcatg cctagagcta tgggtcgtgc aactacaata
     3841 agaaaaagaa cttctcatat agaggttgtt gttaaagaaa aaaaataatt aatagatagg
     3901 agggaaagta atgggtcaaa aggttaatcc acacggctta agggtcggtg ttataaaaga
     3961 ttgggactca agatggttcg ctactgataa aaaagagttc ggaaacttat tattagaaga
     4021 ccataatata cgtaaattct taaagaaaag attatactca gctggagttg ctaagataga
     4081 aatagaaaga tcagctaaca aaataaaaat ggacttacac gttgctaagc ctggtgtagt
     4141 tataggaaga gctggtgcag gaatcgaagc attaaaagct gaattagaaa aaatgacaaa
     4201 gaaaactata atagttaata tagtagaagt aagaagtaca gataaaaatg ctcaattagt
     4261 agctgaaaat atagctttag ctatagagag aagggttgct ttcagaagag caatgaaaca
     4321 agctatacaa agagcaatga aatctggtgc taaagggata aaagtttctg cttcaggaag
     4381 attaggtgga gctgaaatgg ctagaactga aggttatagt gaaggaaatg tgccactaca
     4441 aactttaaga gcagatatag attatggttt cgctgaagct gatacaactt atggaaaaat
     4501 cggtataaaa gtttggatat gtaatggtga agttttacca actagagacg gtgtaaatcc
     4561 aagagaagaa agcagaaaga gcgatagaag agacaacaag agagataata gaagaaacga
     4621 tagaagagga aatgacagaa gaggaaatga taacagagga aactacagag gacaaagacc
     4681 tcaaggtgga tcaagacctc aaagaactga aaacaaagga aattaataat agttaggttc
     4741 aaggaaggag gaaaaatact catgttaatg ccaaaaagag taaagcgtcg tagagttcat
     4801 agaggaagca tggctgggca agctcataag ggtaataaag ttacttatgg tgagtttgga
     4861 ttagtagcat tagaagcttc ttggataact tctaatcaaa tagaagctgc cagaatcgcg
     4921 atgactagat atataaaaag agggggaaag gtttggataa aaatattccc tcataaacca
     4981 gtaacaagaa aaccagcaga gactcgtatg ggtgcaggga aaggttctcc agaatattgg
     5041 gtagctgtag ttaagccagg aagagttatg tttgaattag caggtgtttc tgaagataaa
     5101 gcaagagaag ctatgagact tgctgcacat aaattaccaa tcaaatgtaa atttgttaaa
     5161 aaagaagatt tagaagtaaa gggtggtgaa taggatgaaa gctaaagaat taagagattt
     5221 aacaagcgaa gagctaatga acaaattaaa cgacttcaaa agtgaattat ttagcttaag
     5281 attccaatta gctactggtc aattagaaaa tacagctaga ataaagtttg ttaagaagga
     5341 tatagcaaaa gttaaaactg ttcttgctga aagaaagtta tacgaaacta gagcttaatt
     5401 tggaaaggag gctttttaaa catggaaaga ggaagaagaa aagttagaat aggccgtgtt
     5461 gttagtgata agatggataa aacaatagta gtagctgttg aagaattcgt acgtcatcca
     5521 ctatataata aacgtgttaa gagaactaag aaattcaagg ctcatgatga aaagaatata
     5581 tgtaacatcg gagatagagt taaaataatg gaaactagac cattatctaa agataagaga
     5641 ttcagactag ttgaagttgt tgagaaagtt aagtagtttt tgaagaagga gggattacga
     5701 tatgatacaa caagaatcac gtctaagagt tgctgataac tcaggagcta aagaactttt
     5761 atgtatacgt gttctaggcg gaagtaaaag aagatatggt aacataggcg acgttatagt
     5821 tgctactgtt aaaagtgcaa cacctggtgg agttgtaaaa aaaggtaagg ttgttaaagc
     5881 tgttatagta agaagcaaac aaggcgtaag acgtaatgac ggaagctata tatcttttga
     5941 tgaaaatgct gctgttataa taaaggacga taaaactcca gtaggaactc gtatattcgg
     6001 gcctgttgct agagagttaa gagacaatga attcatgaaa atagtttctc ttgctccaga
     6061 agtactataa ttaaggaggt gcaataggac atgatgcgtg ttaaaaaagg tgacactgtt
     6121 gtagttatag caggtaaaga taaaggtaaa aaaggttcag ttttaaaggt atatcctaaa
     6181 actagcaaag tgttagttga aggtgtaaat gtaataacta aacatcaaaa accaagtgct
     6241 atgaaccaac aaggtggaat aataaataaa gaagccccaa tacacatatc taatgtaatg
     6301 ccatttgatc ctgaaacagg aaaaggcgtt agagtaagat atgaagtaaa agatgggaac
     6361 aaagtaagag tatcagcaaa gagcggaaaa gaattataat aaatattgct tgaaaggagg
     6421 gaccttaaat ggcttctaga ttacaagaaa aatacatgaa agaagttgct cctgctttaa
     6481 tggagaaatt tggatacaaa aacgtaatgg agatacctaa gttaaataaa atagttatta
     6541 acatgggtat aggtgacgct agagaaaatc caaaaggatt agaaaaaggc gttgaagaat
     6601 tagaaatgat atcaggacaa aagcctgtta taactaaagc tagaaaatct gttgctaact
     6661 tcaaattaag agaaggaatg ccaataggaa caaaagttac attaagagct gacaaaatgt
     6721 tctacttcat ggacaaatta gtttcagttt ctttaccaag agttagagac tttagaggag
     6781 ttaaccctaa tgcttttgat ggtagaggaa attatgcttt aggagttaaa gaacaattaa
     6841 tattccctga aatagaatat gataaaatag ataaagtaag aggaatggat ataatatttg
     6901 ttactacagc aaaaactgac gaagaagctc gtgaattatt aaaattatta ggaatgccat
     6961 tttctaagta agcaaaggag ggattcccgt ggctagaaaa gcgatggttg ttaagcaaca
     7021 aagaaagcaa aagtacgcta ctagagaata cactagatgt actatatgcg gaagaccgca
     7081 ttcagttttg aaaaaatttg gtatatgccg tatatgcttt agagaattag cttataaagg
     7141 tcaaatacct ggtgttagaa aagcaagctg gtagaagctt gattagaatg gaaggagggc
     7201 aacaaaatga caatgacaga tccaattgca gatatgctta ctcgtataag aaatgctaat
     7261 gttgttaagc atgaaactgt agatgttcca gcttctaata tgaagaaaga attagctaga
     7321 atcttattag aagaaggttt cataagaggt tatgatgtta tagaagatgg aaaacaaggt
     7381 atcataagaa tacaattaaa gtacggacaa gaaggcgaaa gagttataac aggtcttaag
     7441 aaaatatcta agcctgggat gagagtttac gctgctaacc atgagatacc aaaagtatta
     7501 aacggattag gaatatcagt tatatctact tcaaaaggaa tattaactga caagcaagct
     7561 agaaaagaaa atgttggtgg agaagtaatc tgctacgttt ggtaattaat atgaacaatg
     7621 taaggaggtg cgaccatgtc aagaataggt gttaagccaa taataatacc agcaggtgtt
     7681 gaagttacta tagctgaagg aaatttagtt acagtaaaag gtccaaaagg aactttaact
     7741 aaacaattaa gtgctgagtt aaacataaaa aaagaagaaa atacaataat ggttgaaaga
     7801 ccaactgata ataaaaaaca tagatcttta cacggactaa ctagaacttt attagataat
     7861 atggttgtag gtgtaaatac tggtttcgaa aagaaattag aattaaaagg tgttggatat
     7921 agagctcaaa aacaaggaaa gaaattagtt atgaacttag gtttctctca tccagttgag
     7981 atggaagatc cagaaggaat aactgttgaa gctccaaacc aaacagagtt aatagtaaaa
     8041 ggtatagaca agcaattagt agggaattat gctgctaaga taagagcttg gagaaagcca
     8101 gagccataca aaggtaaagg tataaaatac gttgatgaag taatcagacg taaagaagga
     8161 aaaactggta aaaaataata ccaaaactta aacgctagaa aggagtgagc taggtgttaa
     8221 aaaaggctga taaaaatgct aatagacttc aaagacataa gagagttcgt agaaaaataa
     8281 gcggtacttc tcaaagacca agattatgtg tatttagaag tgctaacaat atatatgctc
     8341 aaataataga tgacacaaaa agagtaactc tagttgctgc atcttcttta gaagcagaag
     8401 ttaagagtgc tgtcaatcat acaggaaaca aggaagcagc taagaaagtt ggagaattag
     8461 ttgctaaaaa agctgttgaa aaaggaatca ctgaagttgt atttgacaga ggtggatact
     8521 tatatcatgg aagaattcaa gaattagctg aaggtgctag agaagctggt cttaagttct
     8581 aatacgaagg agggaaaaat acatgctacg tcgtaagccg atagatgcag gacaacttga
     8641 cttacaagaa aaagttgttg aagtaagacg tgttactaag gttgtaaaag gtggtagaaa
     8701 tttcagattt gcagcattag tagttgttgg agatgaaaac ggacacgttg gtataggtgc
     8761 tgggaaagca atggaagtac cagacgctat aaaaaaagca gttgaagatg ctaagaaaaa
     8821 tctaatagta gtgccaatag ttgggactac aattcctcac gaagtacgcg gacactttgg
     8881 tgctggaaat atattaataa tgcctgctgt agaaggtaca ggagtaatag ctgggggacc
     8941 tgctagagcc gtacttgagc tagctggact aaaagacgtt agagctaaat ctttagggtc
     9001 taacaatcca agaaatatgg taaatgctac aatagaagga cttaattctc taaaaacagt
     9061 tgaagatata gctaaactta gaggtaaaaa agttgaagaa cttctagggt aaggaggtag
     9121 tgtaaaatgg ctaaattaca aataaagtta gttagaagtg ttataggaac tactcctaac
     9181 caaaaaaaga atgttgaagc gttaggatta agaaaaagag aacaagttgt tgtaaaagaa
     9241 gacaatgctc aaacaagagg tatgataaat aaagtaagtc acttattaga agtaactgaa
     9301 atagctgaat aatttttata aagtagtgca aggaggtgca atccatgaag ttacatgagt
     9361 taaaacctgc tgaaggtgca gtaagagcta agagaagatt aggtagaggt actgcaactg
     9421 gacaaggtaa aactgcagga cgtggacaaa aaggtcaatg gtctcgttct ggtggtggag
     9481 taagagtagg atttgaaggt ggacaaatgc cactagctag aagacttcct aagagaggtt
     9541 tcaataacat atttaagaaa gtttacacag aagttaatgt tgaagtttta aacagatttg
     9601 aaaatggaac agaaataact gcagaattat taaaatctac taaaactata agcaaaatag
     9661 gtaaagacgg aattaaaatc ttaggtgaag gtaatttaga gaaagcttta actgttaaag
     9721 ctgctaaatt tacagcttca gctcaagaaa aaatagaaaa agctggtgga aaagcagagt
     9781 tagtataata acctgctaat ctcctagcca gtacaggcgg ggtgaattag cgtgctgtca
     9841 aaattaaaac aagcttggaa gattaaagca gtaagaaaaa aagtaatgta tacacttatg
     9901 atgatcgtga tattcagaat aggtactact ataccagttc caggcataga cactagtatt
     9961 atacaaaaga tggtaggcgg caatagtcta ctttctctgt ataatatgtt tactggtgga
    10021 gcatttagta acttttcact atttgcttta ggtataagtc catatatcac tgcatcaatc
    10081 ataatacaac ttctaacagt aggttttgaa agtttagcag aacttcaaaa atctggtgaa
    10141 gaaggaaaga aaaaaatcaa taagtacact aaatatacag cactagctct agcagttgta
    10201 caagctttgg gaattacatt aggaattgta agaagtgctt taatatcaaa tagtgtgttt
    10261 tttatcacaa ctgttgtaat aacattagtt tcagcaagta tgttagtaat gtggattggg
    10321 gacaaaatta ctgaaaaagg aatcggtaat ggtagttcag taataatatt tgcaggtata
    10381 atatcaagaa tacctacaga tgttattaaa atatcgcaac aagttaaatc tggcgaagtt
    10441 gcaccttggg ttatagtgat tctagctgta gttatattac ttacagtaac aggagttaca
    10501 tttatacaag aagcaactag aaagatacct gtacaatatg ctaaaagagt tgttggaaga
    10561 aaaatgtatg gaggacaaag ttctcatata ccaatgaaag ttaaccagtc aggagttatg
    10621 cctataattt ttgctagttc acttttagct ttccctcaaa caatagctat gtttatgggt
    10681 ccaaatgctc aggcttttgt tcaaaaatac ttaagtatgg caacagaaca aggattttgg
    10741 acatatagat caattgagat tctacttata attttcttct catattttta tacaactgta
    10801 tcatttaata cagaagacat atctaaaaat atgaaaaata atggtggatt tataccaggt
    10861 ataagaccag gtgagcctac tatggattat ttaaatagaa ttttaactag attaacttta
    10921 gctggagcta ctttcttagc aataattgct atggtgccag ctcttacaac acattatatg
    10981 aaagtaaata tgagtctagc tgggacttca ttgctaatcg ttgttggagt tgctttggag
    11041 cttaagagac agttagagtc aaacttagtt atgagaagct atcaaggttt cttaaaataa
    11101 aatggagatg gataatatga gaataatatt acttggacct ccaggtgctg gtaaaggtac
    11161 tcaggcggca ggaatagtag aaaaatacaa tatacctcat atatcaacag gagatatatt
    11221 cagaaagaat ataaaagagg gaacagaact tggaaaaaaa gctaaagaat acatggacca
    11281 aggtttatta gtaccagatg agttaactgt aggtttagtt actgatagaa tatctcaaga
    11341 agattgtaaa aatggattta tgttagatgg atttccaaga aatgtagcac aaggagaaca
    11401 tttagatatc ttcttaaaaa atgctggtat atcactagat aaagttgtca atattgaagt
    11461 tgataagagt atattagtgt ctagagcagt tggtagaaga atatgtaagt cttgtggagc
    11521 tacttaccat gttgagttta atcctcctaa agtagaaggt gtatgtgatg tatgccaagg
    11581 agaattatat caaagagctg atgataatga agaaactgta tctaagagaa tacaagttta
    11641 tctagatgaa actaagcctt tagtagatta ttatagcaaa caaggtataa tagcagatat
    11701 aaaaggtgat caagcaatag ataaagtatt tgaagatatt gtcgcagcct taggaagtgg
    11761 aaaataaatg attatcttga aatcaaaaaa agaaatagaa cttttaagag aagcaggtaa
    11821 aatagttgct gacactcatg aagttttaag aaaagctatt tctccgggaa tatctacttt
    11881 agaattagat aaaatagctg aagagaatat aagaaaatac aatgcagaac cttcttttaa
    11941 aggatatgga ggttttccag gaagtatatg tgcttctatc aatagagagg tagtacatgg
    12001 tattccagga gaaactatat tacaagaagg tgacattgta agtttggata taggagccta
    12061 ttataaagga tatcatggtg attctgcgaa aactcacgga gtaggcatga tatctgaaga
    12121 agataggaaa ttgatagaag taactagaga aagcttctat gaaggaataa aatttgctaa
    12181 attaggatat agactttcag atatctcaca cgcagtacaa acacacgttg agaaacatgg
    12241 tttctcggta gttagagatt tagtgggtca tggagttgga gcaaatcttc atgaagaccc
    12301 acaagtacct aattatggac tcccaggaaa aggaccaaga cttagagaag gaatggttat
    12361 tgctatagag ccaatggtta atgcaggtcg ttatcatgta aagactctat cagatgggtg
    12421 gacgactgtt acaatagatg gaaaaaaatc agctcattat gagcatacga tagcaattac
    12481 tgaacatgaa cctttgatat tgacaaagtt gtaatttaaa aagtaggtga aaaaagtgct
    12541 atcagataat ttaagtatag gtcaagttgt taaagtttca ttaggacgag ataaaggaaa
    12601 tttattcttt gtagtcaaaa taattaataa tgaatatgta ttaatagctg atggaaaaaa
    12661 gagaaaactt gacaaaccta agttaaagaa agtgaagcac ttaaaaaaat ataattttat
    12721 taatgatgaa gttagaaaaa gagtagtatc tggacaagag attacagatt cttttttaag
    12781 agctgaactt actaagttaa attagataat ttaacttatt gaaatggagg gctggttagt
    12841 taatggccaa aaaagatgtt atagaattag aaggtacagt ttcagaagct ttacctaatg
    12901 ctatgttcaa agttaaacta gaaaatggac atgagatatt atgccacatt tctggaaagc
    12961 taagaatgaa cttcataaga attcttgaag gtgacaaggt gaatgttgaa ctttctccat
    13021 atgaccttac aagaggaaga attacttggc gtaagaagta gactatatta atatttagtc
    13081 taaggaggga ttaataatga aggttagacc atcagtaaaa ccaatatgtg aaaaatgtaa
    13141 agtaataaaa agaaaaggaa aagtaatggt tatctgtgaa aatccaaagc ataagcaaaa
    13201 gcaaggataa gaaattatta ctagcatttt gttaaatttt atagtataat actatattgt
    13261 gatgttaaca aaagaacgtt aaaatcgttg aacaatctgg cgtaggattg gcatatgatg
    13321 tgaagtttta aacactaaaa actatgtgta ggaggtgcga tcatggcaag aatagctggg
    13381 gtagatttac ctagagaaaa aagagcggag ataggcttaa cttatatata tggtataggt
    13441 aaagcaactg ctaacgaaat attagctaag gctgagataa atccagatac tagaataaaa
    13501 gatttatcag aagatcaagt taatgagtta agaaaagtta tagatgacga tttcttagtt
    13561 gaaggtgact taagaagaga aattgcttta aacataaaga gattaagaga tataaaatgt
    13621 tatagaggaa taagacacgc taaaggtctt ccattaagag gacaaagaac taagactaat
    13681 gctagaacta gaaaaggtcc tagaaaaaca gtatctcgta aaaagaaaaa ataaataaat
    13741 aataactaga tgaaggaggg aaaaggaaaa tggctaaacc aaaaaagaaa gttacacgta
    13801 ttagaagaag agaacgtaaa aatatagaac gtggtcatgc tcatatacaa tcaactttca
    13861 ataatacaat aataacttta actgacgttc acggaaatgc tatatcttgg gcaagttctg
    13921 gacaattagg attcaaagga tcaagaaaat caactccatt tgcatctcaa atggctgctg
    13981 aaacagctgc aaaagctgcg atggaacacg gactaaaaag tgttgaggta ttcgtaaagg
    14041 ggccaggttc aggaagagaa gctgcaataa gagctttaca agcaactgga ctagaagtaa
    14101 ctatgataaa agacgttact ccaatcccac ataacggatg cagaccacca aagagaagaa
    14161 gagtgtaatt aataagagta caataggagg tgtagataaa tggcaagata tacaggtgca
    14221 tcatgtagac aatgccgtag agagggaatg aaattattcc ttaaaggtga tagatgttat
    14281 acagacaaat gtgctatagt aaaaagaaac tatgctccag gtcaacatgg ccaaggaaga
    14341 aagaaagttt ctaactatgg attacaatta agagaaaaac aaaaagttaa aagaatatat
    14401 ggagttttag agactcaatt tagaaattta tatgaacgtg ctgaaaatat gcctggtaaa
    14461 gcaggtgaga acttattaag tttattagag agaagattag ataatgtagt ttacagaatg
    14521 ggactagcat cttctagaaa agaagctaga caattagtga ctcatggtca tttcacttta
    14581 aatggaaata aagtagacat accttcatta atagttaagg ttggagatgt tatagaagtt
    14641 aaagagaaat caagatcttc tgcaaaattc aaaaatttag tagaagttaa ctcaagaata
    14701 gctcctaagt ggttagaagc taatgtagaa ggaatgacag caaaagtagt tggtgttcca
    14761 acaagagaag atatagatct tgagatagca gagcacttaa tcatagaact ttactctaaa
    14821 taataagcac acttttaaaa attattttaa aattttgttt accctcagtg gattactgaa
    14881 ttttaaggag ggttttgtcc atgatagaaa tagaaaagcc aaaagtagat atagttgagc
    14941 ttagcgaaga ctatagatat ggtaagtttg ttatagagcc tctagaaaga ggatatggaa
    15001 taactatagg taacgcatta agaagaatat tattatcatc attaccaggt gtagcagtga
    15061 atgctataaa aatagatgga gttcttcatg aattttcaac aatacctggt gttaaagaag
    15121 atgttactga gataatatta actttaaaag agctttcagc aactatagat ggtgaaggaa
    15181 gtagaacact taaaatagaa gctcaaggac catgctctat cacaggtgca gatataatct
    15241 gtcctccaga tgttgaaata ttaagtaaag atttagcaat agctacatta gatgataatg
    15301 ctaaacttaa tatggagata tttgtagata aaggtagagg ttatgtttct gctgaagaaa
    15361 ataaaacaga gaatgttcca ataggtgttt tacctgtgga ttcaatatat actcctgttg
    15421 agaaggttag ttatcatgtg gaaaacacaa gagttggtca aaaaacagat tatgataaat
    15481 tagtactaga agtttggact aatggtagta taaatcctca agagggaata tcattagctg
    15541 caaaggtatt agttgagcat ttaaacttat ttatagactt aactgaacat gtaagtagtg
    15601 ttgaaatcat ggtagaaaaa gaagaagatc aaaaagaaaa agttcttgaa atgactatag
    15661 aagaattaga tttatcagtt agatcatata actgtttaaa gagagcggga attaatacag
    15721 ttgaagaatt agctaataaa tctgaggatg atatgatgaa ggttagaaac ctaggtaaga
    15781 agtcattaga agaggtaata caaaaactag aagaacttgg attgggatta aaaccaagtg
    15841 aagaatagcg agcaaaggag ggataagaat ggctaagtac cgtaaattag gacgtgaaac
    15901 agctcacaga aaccttatgt taagaaactt agtaacttgc ttactaagaa gtggaagaat
    15961 agaaactaca gtgactagag cgaaagaaac tcgtagaatg gctgaaaaga tgataactct
    16021 tgctaagaga ggagatcttc atgctagaag acaagtttta gcttatgtta tggatgaaac
    16081 agtagttaat aacttattca cagatttagc tccaaaatat gctgagagaa atggtggata
    16141 cactagaata ataaaaatag ggccaagaaa aggcgatgct gctgaaatgg cttttataga
    16201 attagtatag taaggaaagg attaggcttg atggcttaat ctttttttta tttttttgac
    16261 taaggtatga tataataatg tgtaaaatga tttttataag ttattgctta ctaaaaggag
    16321 tatgagttaa tggataatat agtaaaggta aataatattt cgtttgagta tattacagat
    16381 gaagcaaaac ttaaagcaat agataattta agcctagatg ttaaaaaagg agaatttgtt
    16441 gcgataatag gacataatgg ttctggtaag tctactttat ctaaaaactt aaatgctatt
    16501 cttatgccta ctgaaggaaa tattcttatt gatgatatgg atactaaaga agaagaaaga
    16561 ttatgggata taagacaaac tgcaggcatg gtatttcaaa atccagataa tcaaatagtt
    16621 gctactatag tagaagaaga tgttgcattt ggacctgaga acttaggtat agaaccaaaa
    16681 gaaataagac gaattgtaga ggagtcttta aaaagtgtag gtatgtatga tttaagagat
    16741 agacagcctc atttgttatc aggaggtcaa aagcaaagag ttgcaatagc aggaattatt
    16801 gctatgaggc ctaaatgtat aatatttgat gaagcaacag caatgttaga tccatctgga
    16861 agaaaagaag ttatgaaaac aataaaaaga cttaataagg aagaaaatat aactgttata
    16921 catataacgc actttatgga agaagcagta gaagctgata gagttgtagt tatggaaaaa
    16981 ggaaagaaaa tcttagaggg aactcctaga gaagtattca gtaaaataaa gatgttaaag
    17041 gaaatcggtt tagatgtacc ttgtatgaca gagttatcta gtttattaat agaagaaggt
    17101 ataaatatta gtagtgatat attaactgtg gatgagatgg tgatggaatt atgtcaatta
    17161 tagtaaagaa tttaacacat atatataatg aagggatgcc ttttgcaagt aaggcacttg
    17221 atgatgtttc ttttgagatt aaagatagag attttgttgg tcttataggt catacaggct
    17281 caggaaaatc tacattaata caacatttaa atggattact aaagccttcc tcaggagaaa
    17341 tatttataaa tgattttaat ataacagata aaaacttgaa tttaactgag attagaaaaa
    17401 gagtaggggt tgtatttcaa tatccagaat accaattgtt tgaagaaacg attgataaag
    17461 atatagcttt tggaccctct aatttaggct tagaggagtc tgagatacat aacagggtaa
    17521 aagcatcaat ggaagctgta ggacttgatt atgagggatt taaagacaaa tctccttttg
    17581 aattatctgg gggacaaaag cgtagagtag caattgctgg agttatagcc atgaacccag
    17641 aagttctcat attggatgaa ccaactgctg gtcttgaccc tggtggaaga gatgaaattt
    17701 ttaacttaat aaaagactta catgaaaaaa agaatatgac tataatacta tcatcacata
    17761 gtatggatga tatggcaaag ttagcaaaaa ctttaattgt tatgaatcat ggtagcgtag
    17821 aatttatggg aactcctaga gaagtattta aatcaaatgc tagtaaatta aaagatatag
    17881 gattagatat tccacaagtt ttagagcttg cattgaaatt gagagaaaaa ggctttgaca
    17941 tcagtgagga tatattgact ttagaagaag caaaacagga aatattaaaa gttgtgagag
    18001 gacgaggatt atgttaaaag atataactat agggcaatac tacccaacaa gttctgctat
    18061 tcataaatta gatccaagga taaagcttgt agcgacaatt gtatttatgg tatctatatt
    18121 tgtagtaaat aaattttggc cttatatagt agttttatta tgtttattag ctatgataaa
    18181 attggctaat ataccagtca aatatattgt taagggtgtt aaacctctta aatggattat
    18241 tttgtttaca tttcttataa atattttttt cttaccaggt gatgaaatat ggtcatttgg
    18301 atttttagct attacaaaac agggtctaag acaagcaatt tttatggcaa taagattaat
    18361 atttttagta gttggtacat cattgcttac tttaacaact tcaccaatag aattaactga
    18421 tggaatagag agattgttga atccatttaa gaaaattggt ttgccagttc atgaacttgc
    18481 aatgatgatg acaattgctt tacgttttat tcctacattg ttagatgaga cagacaaaat
    18541 aatgaaggct cagatgtcta gaggtgcaga ttttgaaagt aagaatctta taaatagagc
    18601 aaagaattta gtaccacttt tggtaccttt atttgtaagt gcatttagaa gagctgatga
    18661 attggctatg gccatggagg ccagatgtta cagaggtgga cataatagga ctaagatgag
    18721 agagtctgtt atatcaagaa gggattatat ggcatgtgta tttcaggttg tttatctggg
    18781 agcaattata gctactagat ttatagcaat ttaattaata agttatagat ataatgaggt
    18841 aaagtatgag aaatataaaa attaaaatcc aatacaatgg taagaattat tgtggatggc
    18901 aaaagcaacc tgattcttta gggatacaag gtacaattga aagggctata tatgatataa
    18961 caaaagaaga aaccagtctt atagggtctg gtagaactga ttcaggagtt catgctattg
    19021 gtcagattgc aaattttaaa attaattcag gtataagtat agaaagtata ccaatggcac
    19081 taaatgccaa gttgccaaaa gacatatcag ttatagaagc atgtgaagta aatgatgatt
    19141 ttcactccag atacagtgca aaaggtaaaa catataaata tttagtatat aatagcaagt
    19201 ttagaaatcc aatacttagt gagatatcat atcaagtaaa atatgaactt gattttgata
    19261 aaatgtgttc agaagcaaaa agtttattag gaacacatga ttttaaaggt tttatgagtt
    19321 ctggttcatc tgtaaaagat acagttagaa cgatatatga tattgatata agtaaaaaag
    19381 atgacttgat tacttttgag attagtggta atggtttttt atataacatg gtgaggatta
    19441 ttgttggaac acttgttgat atgggtcgtg gcagaattaa cgaaccattt ttagatataa
    19501 tacaatcaaa aactaggtca agatgtggac atacagctcc agcacaagga ttatttctca
    19561 aaaaagttca ttattaactt gacacaccag gctacatgta ttaaaatata tgagaatgtg
    19621 ttaataagtc cacttgcccc ggacttgaca taaacggtaa aaaaatttta tgaaaagtag
    19681 ttaaggaggg acagttatga aaagttatat agctaagcca gctgatgtac aaagaaaatg
    19741 gtacttagtt gatgctgaag gaaaaacatt aggtcgttta gcaacagaaa ttgcgacagt
    19801 attgagaggt aaacataaac caacatttac tcctcacgtt gatggtggag attttgttgt
    19861 tgtagtaaat gcagaaaaaa tagttttaag tggtaagaaa ttagatcaaa aatattacag
    19921 ataccatact ggttatgtag gtggattaaa agaaatatct tacagagata tgatggataa
    19981 gaaaccagaa gaagttatat cacatgctgt aagcggtatg ttacctaaaa ataaattaag
    20041 aagcagaatg atgactagat taagagtatt tgcaggtgct gagcacactc atgcagctca
    20101 aaatccagaa gttttaaact ttaaataaga ttaataggtg aaaggaggag tttataatgg
    20161 ctaacgttca atattatgga actggaagaa gaaaaagttc tgttgctaga gtaagactag
    20221 ttgcaggtga aggaaatata ttagtaaatg gaagagcatt agaaaattat tttaattatg
    20281 agacattaat aagagatgtt aaacaaccat tagttttaac tggtaatgaa aataaatatg
    20341 atgttatagt aaaagttgaa ggtggaggat tcactggaca agctggagct ataagacatg
    20401 gaatatctag agctttatta aaagctgatt tagatttaag acctgcttta aagaaagaag
    20461 gtttcttaac tagagatgca agaatgaagg aaagaaagaa atacggatta aaagcagcaa
    20521 gaagagctcc acaattctca aaaagataat tacttatttt tttggaaaag caaaatcctt
    20581 tatttactgt tattcagaaa gtattggtat tttaaaaatg ttgggttaag ttaattattg
    20641 atatataata aaataataag atatcttatt aggatgtttt tcattaagaa gaacatcctt
    20701 tttatttt
//
LOCUS       DBGJFF010000064        20483 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.065, whole genome shotgun sequence.
ACCESSION   DBGJFF010000064 DBGJFF010000000
VERSION     DBGJFF010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20483
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.065"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     repeat_region   118..534
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=184..206
                     /rpt_unit_seq="ataccacttagttaatataaaac"
     gene            complement(814..966)
                     /locus_tag="ACOIAF_002333"
     CDS             complement(814..966)
                     /locus_tag="ACOIAF_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669342.1"
                     /translation="MDNFLQGVLASLVASLIVYLNSKLFKKVKSHSGRSDFSFELKIK
                     FKKNKH"
     gene            2129..3484
                     /locus_tag="ACOIAF_002334"
     CDS             2129..3484
                     /locus_tag="ACOIAF_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HGZ2669343.1"
                     /translation="MDTKISINENMSLGKRFFKYLAPSVVAMWVFSLYTMVDGIFVSK
                     GVGELALAAVNISMPFINFIFAVSLLFSTGASTIIAIYLGKKDIKSANEVFSFNLVSI
                     IILSIIILAITFFNLDRLALFLGATESTIGMVKDYLGIIIFFNGFFIVSYSLEVIIKT
                     DGFPILATVGVIISALTNIILDYLFVIEFGWGVKGAGIATGLSQVFSTIFFLIHFLRK
                     NSTLNFSKFRIDFKTLRKIVFIGFPDSTTELSCGIVVLLFNLSLTKYIGENALIYYSV
                     INYINTLVLMTMMGITQGMQPLTSFYYGAGNIDNVKKLLKMGIKATIIASVAVFAICM
                     AFSGPIVSLFIHPEETMLFNEGVRVFKIFSISFLLVGINVIISGFFVSVEKPSISTVI
                     SLGRGLVIVVLSLISMILIFGGQGIWMTTIVSEFICLVLSLVFLKKNFSTLDSNLNKV
                     A"
     gene            complement(3602..4261)
                     /locus_tag="ACOIAF_002335"
     CDS             complement(3602..4261)
                     /locus_tag="ACOIAF_002335"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HGZ2669344.1"
                     /translation="MLKLADEKFVMSFSGGKDSILALNRMLKKGYKPVALLTTISEEH
                     GKSWTHNLEYNMLKQVSSNIGLPLLVAECGVEGYEESFERALIKAKNMGATICAYGDI
                     DIESHRKWDTDRCEAVGMKVDLPLWQESREDLVYEFIDSGFCSVVTKVNLKHLGEEFL
                     GKKLTRELVEKIKNAGADPCGEHGEYHTFVVDGPIFKKPVEYEVKGTLIKDGYGYLEI
                     K"
     gene            complement(4337..4765)
                     /locus_tag="ACOIAF_002336"
     CDS             complement(4337..4765)
                     /locus_tag="ACOIAF_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HGZ2669345.1"
                     /translation="MTKLWAIIFALLAGGSTALEAFINGELGKSTTALVATFISLTVG
                     AIFFLASIIVTGDLKCLVSINGFNYKLLLGGVFGGLIIYFTVKAIPNLGVSNTLILTL
                     VSQVLVGFFIDSVILGQETMHLYKYIGVVLLILGTFFIVN"
     gene            4917..5465
                     /locus_tag="ACOIAF_002337"
     CDS             4917..5465
                     /locus_tag="ACOIAF_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HGZ2669346.1"
                     /translation="MYTNFNIDFNNFNKKENAIKFMLMGVLLIILGLLCLTFKTLGIK
                     LISWTFGIALLFFAYLNLKNINELKRYATKEEIKPSINIQWVLIIACILLFVFPQKIQ
                     SIFSLFLGFYLIFNQLVALVNSKNNPYSKFTTWNIVKILFGICLILSPLFLSRFIVSI
                     MSFFIILFGLVLFFSGNTARKY"
     gene            5584..6519
                     /locus_tag="ACOIAF_002338"
     CDS             5584..6519
                     /locus_tag="ACOIAF_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893027.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HGZ2669347.1"
                     /translation="MDKFKYAFDNKRYHTWNYYLRNTFGEKVFKVSINAGFTCPNIDG
                     SLGYGGCTYCSKEGSGDFAGNPKDNLISQFYNIKEMMLKKWPHAKYIGYFQAYTNTYA
                     PLEVLKEKYETILELEDVIGLSISTRPDCLPDDVVEYLSELNKRTNLWVELGLQTIHD
                     STSKIINRGHDYKTFVEGVEKLKSKNIKVVVHIINGLPGEDYNMMMETAKTVGKMGVD
                     GIKIHLLHVIKDTPMEKMLQNGMLTLMEQDEYINLVCDQLEILPETMIIHRLTGDGKR
                     DELVGPIWSLKKWEILNQIDDTLKARNSYQGCKFV"
     gene            complement(6790..7734)
                     /locus_tag="ACOIAF_002339"
     CDS             complement(6790..7734)
                     /locus_tag="ACOIAF_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HGZ2669348.1"
                     /translation="MGEKSKGYVFIAIAGLLWATLGLFGKFLMGNGLTSEQVAFTRLF
                     FGFIVLGVYSSIRTPQILKINKKGIIYSVIIGIICQAMFNLCYFKAIDIAGVSIAAVL
                     LYTSPLFLAIFSKLCYKENITRSKLFSLILCFIGAIMAVTGGRLDFQGLNAFGLLLGV
                     LSAIAYALMPTISKNALKEFSSSTILVYSFLFGAIFMIPSSRPWEILNYAKDLDVLSC
                     MLMLGIVPAALAYIFYAAGISKGVELSVAGVVASVELVGSVIIGCTILGESFSLGKLF
                     GVMLMLISAVVALNLSYDEIRIFYKSNKLKQIEKTESI"
     gene            8052..9482
                     /locus_tag="ACOIAF_002340"
     CDS             8052..9482
                     /locus_tag="ACOIAF_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HGZ2669349.1"
                     /translation="MNLDLEFYQKILEASHDEICVSDDKGIIIYCNKAFEENYGLKKE
                     DILGKNVSFLEDSGYSTKSPIPIVLKTKTKFSLEQDTQTGKKLIITATPIFDENGHLE
                     FTVENCRDITELNNIKNKLEDTKKQVKKYKSEVETLYRTALRIEDTVIMDGIVMRPII
                     NTVNHVSKTDVSVLLLGESGTGKSSLARYIHHNSNRSNGPFITINCATISPQLLESEL
                     FGYTSGAFTGASTKGKVGLVELANGGTLFLDEIGDIPQNLQAKFLQLIQDRTFTPVGS
                     LKNKKVDIRIISATNVDLVSKVKEKKFREDLYYRLNVIEIKLPPLRERRDNLVEIIKY
                     YFNRYSSDFNLNKTISKEAMDAIANYRFPGNIRELQNIIQKILLTCTDNHITIDDLPN
                     ILTKNIHITNNGNKTHISQINKVIISDSKSTNYKNKNFDTLIKEYEKNIILDAYEKFG
                     SSYKVAKHLEISQSKANRLIRKYTNT"
     gene            9682..10266
                     /locus_tag="ACOIAF_002341"
     CDS             9682..10266
                     /locus_tag="ACOIAF_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HGZ2669350.1"
                     /translation="MLMNTHFIMAKSILDNIDENKTFFISEKNFIYGNIKPDAFSKYK
                     LKKHYLDESFNMIVSKINYLCSLNIDSLSKYFSVSRLSQELGVICHFLCDFFCVAHSE
                     RWEFKHSLNKHVTYERELGAFAKDVDLSKIKCGTINSSFEKFFTKLYSDYKKKTDYMN
                     DLKFSAYTCNSVINYILDSILSNTIKSYHIANCG"
     gene            complement(10360..11139)
                     /locus_tag="ACOIAF_002342"
     CDS             complement(10360..11139)
                     /locus_tag="ACOIAF_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HGZ2669351.1"
                     /translation="MIKHIFCDLDGTLYENGTITKEDIVAIEEIEKKGVQFNVATGRI
                     FKQAHNIIKDSLDMNGYYVCENGSFIYDKDYNVIFKRTIDDNLVKKVIDRFESSDAQL
                     YFKYKGDVIVGSDTTAFRHYSSDFIVDPDFEKRSSFDNLIGNIGVCSENLEELSRIEL
                     YLKSEFSEVLEIYFSGTYTLNIVPKSVSKRGSIEHVIKTLNVSPDEVATIGDSPNDIC
                     MLEGFKYSFAMSKAREDVKQSANYVVDSVKSAIDVIMEINS"
     gene            complement(11197..12453)
                     /locus_tag="ACOIAF_002343"
     CDS             complement(11197..12453)
                     /locus_tag="ACOIAF_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HGZ2669352.1"
                     /translation="MKAEIISVGTEILLGDIVNTNSQFLAKELASLGIEVYHQSTVGD
                     NKQRLLECFDESLKRSDFVITTGGLGPTGDDMTKETAAEYFGQKLELHKPSLEVLESF
                     FVKTGKKMAENNMKQVYFPKDAIVLKNNNGTAPGAILKKDGKFIIVLPGPPREMKAMF
                     NESVKPYLQQFTNEMLVSKTLRLYGIGESNLELEILDIINEQTNPTVALYAKELEVTI
                     RITAKAENEREAFKLIKPVEEKIKSRVGKYVYTEGDISISEGETALEDAVSKLLVEKN
                     LTIAVAESCTGGLVSSSLINYPGISSVFLEGCVTYSNDSKMKRLGVKRETLEEFGAVS
                     EQTAIEMAEGVAKGLKANIGISTTGVAGPGGGTKEKPVGLVYTAIYINGKTIVKKNIF
                     NGDRRKIRLRATRDLLNELRIQLEKL"
     gene            12711..13610
                     /locus_tag="ACOIAF_002344"
     CDS             12711..13610
                     /locus_tag="ACOIAF_002344"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HGZ2669353.1"
                     /translation="MKIAVIMGGISSEREVSLNSGKEIYNNLDKNKYEVVKVIIDDKK
                     DIFTKIPEDIDFAILALHGKFGEDGCIQSILETMDIPYSGCGPLCSGMCMDKNITKKM
                     LRDSNLPTAPWVLVKSVDEIDYDEIDNIGYPVFIKPNSGGSSVATFFIHSKDEVEEAV
                     RKGLEVDEFVMIEKYIPGGEYTSFILNGEVFPTISIKSDSGFFDYEAKYSVEKGAKEE
                     VVYLDEELQKRVNEISETCWKIFNCKAYVRVDMIISEGIPYVLELNTLPGMTQTSLIP
                     RSAAARGIKYSELLDKLIEYSLN"
     gene            13634..15082
                     /gene="ddlR"
                     /locus_tag="ACOIAF_002345"
     CDS             13634..15082
                     /gene="ddlR"
                     /locus_tag="ACOIAF_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator DdlR"
                     /protein_id="HGZ2669354.1"
                     /translation="MIFSNLKLNDNEPIYIQLKNYISDMISKGLIPDNSKLPSTRELS
                     QLLQVSRNSVVLTYEELKSEGLIYSISGKGTFVKSKNKSSNTTWSLNWDCLENTYSKK
                     ANELDIIKSEIPWSSDLISFKSISPDGDLFDMEELKKSFLNRISLEGHKLLNYGYAQG
                     YKPLIDYLLEYMTNKGADISNKDILITNGFTEGFDIIMSSFTQEKDYIICENPTHNTA
                     IKIMKSHNLNILGVDINEDGLDFNMLEDNLIKYKNKIKFAYITPSYHNPTGIVMTPEN
                     RYKFYNLMKKYNIAIIEDGFNEELLYNSSHIFPICSLDNMNNGVVYIGSFSKILFPGM
                     RIGWIFADKKVINKLESVKRCRNIHVSFLDQGILFDYLSNGGFEKYIKKIRKFYGDKF
                     NFAYKCIKKYIKTEYILGDGGLHIFIKLKNIDTRVLLEKCYEKNVIFMPGDLFFTDNS
                     GFDTLRLGFSRLSFEDIEIGIKIIGETINEMF"
     gene            15165..15947
                     /locus_tag="ACOIAF_002346"
     CDS             15165..15947
                     /locus_tag="ACOIAF_002346"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HGZ2669355.1"
                     /translation="MSKKSKRRKINKLKLTIVIIVLFLALLSIYEILFSNPNKKGNIK
                     SQETMQNKNNTTDDSKNNQSSTKKQEDNNIKPTFSYSSIPDNIKNKMIGKSMPTDEPI
                     SFDSLSYLKLTYYGFDEKTHQGEMIVNSELAPEVVDIFKELYEKKYPIEKIKLIDDYD
                     AVDEKSMSDNNTSSFCYRTIAGTNVVSNHGKGRAIDINPLQNPQVSGNDVTPKVSTVY
                     ADRSSTKFGMIKKGDDCYNAFVSRGWSWGGYWKNPDYQHFEK"
     gene            15973..17892
                     /locus_tag="ACOIAF_002347"
     CDS             15973..17892
                     /locus_tag="ACOIAF_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HGZ2669356.1"
                     /translation="MNLMTLENISKSYSEKKLLENISLGINEGEKIGLIGVNGTGKST
                     LLKIIAGVEESETGNITKTNGIRIEYLPQNPTYNENSTVLEQVFNGTSEELKLLGEYQ
                     EILEKLNSNFTEELNKKLLSVHEKIDALNLWDLESEAKSVLTKLGINDFNQKIAELSG
                     GQRKRVSLASALITPCEFLILDEPTNHLDNDTIDWLEEYLNSRKGSLLMITHDRYFLD
                     RVTNRIIELDKGRLFSYDGNYSIFLEKKMERLSLEASIEDKRQNLIRTELAWVRRGAQ
                     ARSTKQKARLQRFDELINRDSFKPDENLDISVASSRLGNKIIEIHNISKSFEENTVID
                     NLEYTLTRTDRIGIIGKNGMGKSTLINILNGEIEPDSGYISIGETVKIGCFSQDDSHM
                     NIDMRAIDYVKEASDYIETSDGTRITASQMCERFLFNGTMQYTLIGKLSGGERRRLHL
                     LKILMTAPNVLLLDEPTNDLDIETLKILEEYLDEFKGVVVTVSHDRYFLDRICNKIFA
                     YEGNGKIHIYTGNYSDYLIYREIQGIEFEESKKESVKKDDSTTPKKEKPKNDKKLKFS
                     YNEQREFENIDSDIEKLESKIATLDESTAKFSTDFTKLQEILDEKSKLEKELEYKYER
                     WEYLNNLADEIANNK"
     gene            complement(18134..18481)
                     /locus_tag="ACOIAF_002348"
     CDS             complement(18134..18481)
                     /locus_tag="ACOIAF_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HGZ2669357.1"
                     /translation="MSKSNDVLDIVFKITDGLEYEVAENSVVTILEKQDHKIQKFFRK
                     LKARIPKYKKIELDEYSSFVFLQIDGQRTVREIGKNLEAKYGEESHPLYERLLVFLNH
                     IEVNCHYIERLDI"
     gene            complement(18468..20396)
                     /locus_tag="ACOIAF_002349"
     CDS             complement(18468..20396)
                     /locus_tag="ACOIAF_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT family oligopeptide transporter"
                     /protein_id="HGZ2669358.1"
                     /translation="MNKKLPKGAYGEVSGKDYVPYITDKSRTGGNVAVLIIGIILAAI
                     FAASTTYSGMKAGLTVAAGIPGAIIGSAFVGAFARSKGILGKNLIQGMSSGGESVASG
                     FIFVLPAVILIGSQITFFEGLAVGVGGVLFGIGIAAIVHNYLIVEEHGKLMYPESMAI
                     SETLVASEAGGDSIKYMGIGFVISGFITVLTGSFLNVANNVMSLVGSKFYKWKFDIEV
                     NPLLLGIGFIVGLEVSLTMFAGSILSNFGIAPLIGYFTDMAKDGAMVWNNPAMPLNQM
                     DVGAISSSYVKYIGAGMMLCGGIIGAIKLIPTIIASIKETLKAKSSAGEGEEGSSIQM
                     ILLLGGVVIGFLAAFLISGNIVMAIIGAIISLLLSLLFVIVAGRLTGTIGTSNLPVSG
                     MTIASLVIVTLVFVIMGWTDLEANKSLLLFGSFIVVAIAIAGGYTQSQKVTYIIGGSK
                     NEMQRYFTIASIVGVIVVVGVILLLSDQLRATGDNVQFALPQANLMSTLTSGIMSGSL
                     PWVMIIVGVFMAIVLYALNLPIMTIAIGFYLPIATTSIILVGALIRLFVELVSKTEKE
                     KEVKVSNGISLSSGLVAGGSIIGLIGIILQVTGVVTPKVPSGFAATNSMAIALLVVLV
                     VLTALPIVLSKVKNNEQE"
BASE COUNT         7585 a         2991 c         2692 g         7215 t
ORIGIN      
        1 tagtatcttc tatcttaaaa caaaatgata atttatctat ataaaaaagt gctttgttga
       61 cagagttcac ttttttatat aggtacagaa tttaagtata aatacatgtt ttattctata
      121 cattttagtt aatgtaaaat ttcttgattg tactctctaa cttttcaaca ttatactatt
      181 tatataccac ttagttaata taaaactata aaagtgttgc aattactggg ttttataaag
      241 ttaatttaca taccacttag ttaatataaa acatgtattt atttgttctt gtactattgt
      301 tttactctat ttacatacca cttagttaat ataaaacact ccaaaacttg attctaaatt
      361 tagaaaagga ctatatttac ataccactta gttaatataa aactttggag ttgttacaga
      421 ttatttgcct tatctctcta tttacatacc acttagttaa tataaaactc gaaaaatgga
      481 agaaatataa ggagaggttt aataaattta cataccactt agttaatata aaacccccaa
      541 ataaactggg cattttcaat acttacacat atgcaactct cttaaatttg cagtgaacca
      601 tgagtagtgc aaatgataaa gcttatcata taccctcaac actttatatt tcaagcgtta
      661 agctctactt tgtaacaaat atcgctcact gcaaaactat ttacattttt attatatcat
      721 aaatatatta tttttgaata tctataccaa tttgtggtat aataaaagca agaagaacta
      781 caatctattt agacgtagag tgaagttcaa atactaatgt ttattctttt tgaacttgat
      841 ttttagttca aaactaaagt cactcctgcc agagtggctt tttacttttt taaataattt
      901 actatttaag taaactatta aactggcaac taaacttgct agtacacctt gtaaaaaatt
      961 atccatacat actcacctcc cttctataca ttgggaggat aatcttttgt atgaactcca
     1021 ctctataaat tgtagattac atcttcttgc taaaaatgtt atagcatata attattacat
     1081 attttaccta tatattactt ttctaaagat tcgtctatat atttctgtat acatctactc
     1141 tatcctcgat ttgttacaac cccaatcaaa aaagtagttc aacttctaag ttttatacaa
     1201 agcaggaaac tcaattatta ttagaaaaat caaaaaatac tcctataggt ataccaattt
     1261 ttttgatgct aactcttgga ttacgttttg gtgaagctgt atcacttata tggtcagatg
     1321 tagaccttga taaaaaatct cacatgtaaa tcaaacatta gtttatgtat acaataaaat
     1381 aatcttcaaa gaccctaaaa cccctatatc aaaaagaaaa ttatttgctc ctgatgaact
     1441 tataagtttg cttaaagggg aaaaattaaa acaaaataag ttaaaattac agggtatatt
     1501 gaaaaatgaa tttgatttga tatgcttaaa taaaagattc aacccgtgag ttcaaccaac
     1561 ttttgttgtt cattttaaaa aactattaag agataataat cctagagata taaaagtaca
     1621 tgaattaata catgcaaatg caactttgat attactttca ggtactaaca taaaagctat
     1681 ttataaaata ttagtacatg cagatataaa aatagctatg aatatatatt ccatattctc
     1741 acttattaga tgaaatgaat aaagaaacat ctgaaaatat aagcaaaata ctattcaaat
     1801 aatgttcagt tttatgtcaa ttaaatgtca ctcaaagtgc ttttttttca tttgtcagtt
     1861 aaatgtcact taaagtacct ttttcctatt tgtcagttaa atgtcactta aagtaccttt
     1921 ttcctatttg tcagttaaat gtcagtaata tataaataag atatatttat aagaccctta
     1981 aaatcactat tatttaataa aatttataat tatatactat ttgctgttga cattggtatt
     2041 ataccatagt ttatcattta tattagaaat aaaattatta attaatagtt ttgtaatttt
     2101 ataatagatt tacagggagt gataataaat ggatacaaaa atatctataa acgaaaacat
     2161 gagtctaggt aaaagatttt ttaaatacct agcaccatct gtagttgcaa tgtgggtttt
     2221 ctcactatat acaatggttg atggaatatt tgtaagtaaa ggagttggag aattagcttt
     2281 agctgctgtc aatatatcaa tgccttttat taattttata tttgcagttt ctctattatt
     2341 ttcaactggt gcatctacca taattgcaat ctatctgggg aaaaaagata ttaaatctgc
     2401 taacgaagtc ttttcattta acttagtatc aataattata ttatcaataa ttatattagc
     2461 tataactttt tttaatttag atagattagc actattttta ggagctacag aatctacaat
     2521 tggtatggtt aaagattatt taggaataat aatatttttt aacggattct ttatagtgtc
     2581 ttattcttta gaagttataa taaaaactga tggctttcca atattagcta ctgttggtgt
     2641 aataatatct gctcttacaa acatcatact tgattacttg tttgtaattg aatttggatg
     2701 gggtgtaaaa ggtgctggta ttgcaactgg tttgtctcaa gtcttttcta ctattttctt
     2761 cttaatacat tttttaagaa aaaactctac tttgaatttt tcaaaattta gaattgattt
     2821 taagacactt agaaaaattg tatttatagg ttttcctgat tcaactactg aattaagttg
     2881 tggtatagtt gttttacttt ttaacttaag tcttactaaa tatattggag aaaatgctct
     2941 tatttattat agtgtcataa attatataaa tacattagta cttatgacta tgatgggtat
     3001 aactcaaggt atgcaaccac ttactagctt ttattatggt gcaggaaata tagataatgt
     3061 aaaaaaatta ttaaaaatgg ggataaaagc cacaataata gcatcagttg ctgtatttgc
     3121 aatttgtatg gctttttcag gaccaatagt ctctttattt atacatccag aagaaacaat
     3181 gctattcaat gaaggagttc gtgtatttaa aatattctct atatctttct tattggtagg
     3241 aattaatgta ataatttctg gattctttgt atcagtagaa aaaccatcaa tttctactgt
     3301 tatatctctt ggtagagggc ttgtaatagt tgttttaagt ctaatttcaa tgatactaat
     3361 atttggtgga caaggaatat ggatgactac tattgtttct gagtttatat gtcttgtttt
     3421 atctttagtt tttctaaaaa agaatttttc tacattagac tctaatctaa ataaggttgc
     3481 ttagatgata agagtagcaa tatatgaaaa tatctaaata aaaaatacct attatacaat
     3541 cttgcaaata ttaaaccttt gcaaaaatta ggattatata ataggtattt tatttatgta
     3601 attattttat ttctaaatat ccatagccat cttttattaa agttcctttt acttcatatt
     3661 ctacaggttt tttaaatatt ggaccatcta cgacaaatgt atgatactct ccatgttctc
     3721 cacatgggtc tgcaccagca ttttttatct tctccacaag ttctctagtt aatttttttc
     3781 ctaaaaattc ctctcctaag tgctttaagt ttacttttgt tacaacacta caaaatccac
     3841 tgtctataaa ttcatatact aaatcctctc tactctcttg ccataatggt aaatctactt
     3901 tcataccaac tgcttcacat ctatcagtat cccattttct atgactttct atatctatat
     3961 ctccatatgc acatatcgtt gctcccatgt ttttagcttt tattaaagcc ctctcaaagc
     4021 tctcttcata tccttctacg ccacactcag caactaataa aggtaatcct atgttgctac
     4081 ttacttgctt aagcatatta tattctaaat tatgtgtcca tgactttcca tgttcttcac
     4141 ttatagttgt caagagtgcc acaggcttat atcctttttt caacattcga tttaatgcta
     4201 atatactatc cttaccacca ctaaatgaca ttacaaattt ttcatcagcc aattttaaca
     4261 cctctttcat ctaactttgt tttattaaca taattatacc aaatttttga gttgcatagc
     4321 aaattatatt aaattgttaa ttcactataa agaatgtccc taatattaaa agtacaaccc
     4381 ctatgtattt gtataaatgc attgtttctt gtcccaaaat tacgctatca ataaaaaatc
     4441 caactaaaac ttgggataca agagttaata taagtgtatt tgatactcct aaatttggaa
     4501 ttgcctttac tgtaaaatat attattagac ctccaaatac tcctcctaat aacaacttat
     4561 aattaaaacc atttatagat actagacact ttaagtcacc tgtaactatt atacttgcca
     4621 aaaaaaatat tgctcctaca gttaagctta taaaggtggc cactaatgct gttgtacttt
     4681 ttcctagctc tccatttata aaagcttcaa gagctgtaga accacctgct aataatgcaa
     4741 atattattgc ccataatttt gtcatggaca aacctccttc tatataattc tattaataga
     4801 agtttatgat acaacctatt aattaattac taagtatagt ttaattttta tacactaggc
     4861 tttatttgga tatgaatttc tgttataatt gttctatcga ttttggaggt gattttatgt
     4921 acacaaattt taatatcgat tttaataact ttaataaaaa agaaaatgct attaaattta
     4981 tgttaatggg agtattactc attatattag gattattatg ccttacattt aagacattag
     5041 gtataaaatt aatttcttgg acttttggta ttgcactttt attctttgca tatttaaatt
     5101 taaaaaatat taatgaacta aaaagatatg ctactaaaga agaaattaaa ccctctatta
     5161 atatccaatg ggttctaata attgcatgta tattactttt tgtatttcct caaaaaatac
     5221 aaagcatatt ttcattattt ttaggatttt atttaatctt taatcaacta gtagcacttg
     5281 taaatagtaa gaataatcct tattctaaat ttacaacttg gaatatagtt aagattctat
     5341 ttggtatatg ccttatatta tctccacttt ttttatctag atttatagta tcgataatgt
     5401 cttttttcat aatattattt ggactagttc tatttttctc tggaaataca gcgagaaaat
     5461 attaattatt ttctcgctgt atttttatat tacttaaaat agaatttgtt taagttatct
     5521 atttgtggta atatgttttt tgtacaagct cagcgtacaa ttttattaga aataggtgat
     5581 tttttggata aatttaaata tgcttttgat aataaaagat atcatacttg gaattactat
     5641 cttagaaata cttttggaga aaaagttttt aaggtatcta taaatgcagg atttacttgc
     5701 ccaaacattg atggaagtct tggttatggc ggatgtacct attgtagcaa agaaggctca
     5761 ggtgattttg ctggaaaccc taaagataat cttatcagtc aattttataa tataaaagaa
     5821 atgatgttaa aaaaatggcc tcatgcaaag tatattggct actttcaagc ctatacaaat
     5881 acgtatgctc ctcttgaagt tttaaaagaa aaatatgaga ctatattaga acttgaagat
     5941 gtcataggtc tttcaatatc cacaagacct gactgtttgc cagatgatgt tgtagaatac
     6001 ttgtctgaac taaataaaag aaccaattta tgggttgaac ttggacttca aacaattcat
     6061 gattctacat ctaaaattat aaatagaggt catgattata aaacatttgt tgaaggtgtt
     6121 gaaaaattaa agtctaaaaa tataaaggtt gtcgtacaca ttataaatgg tcttcctggt
     6181 gaagattata atatgatgat ggaaacagca aagacagttg gtaaaatggg tgtagacggt
     6241 ataaaaatcc atctattgca tgttataaaa gatactccta tggaaaagat gttacaaaat
     6301 ggtatgttaa ctcttatgga acaagatgaa tatatcaatc ttgtctgtga ccaattagaa
     6361 atacttcctg aaactatgat aatccataga ctcactggtg atggtaagag ggatgaacta
     6421 gtaggtccta tatggagcct aaaaaaatgg gaaattttaa atcaaattga tgatacttta
     6481 aaggctagaa attcttatca gggctgtaag tttgtttaat acaaaataat aactatagaa
     6541 atggttatat aaatatagtc ctttctatag ttatttagta tttacaaaac aaaatattaa
     6601 tttgtacatt tatacaatat ataataaaaa atagatagtc taaactgtat ctacttatat
     6661 tttatttgta ctgtccactt tattctataa aatttataaa tagttatttt ttaataccaa
     6721 atataatttg gtataatttt tgttggactc taaaatgtat accaataaat cctttttaag
     6781 tatataattc taaatacttt cagttttttc aatctgcttt aacttgttgg acttataaaa
     6841 tattcttatt tcatcataag aaagattaag tgctactact gcagatatta acatcaacat
     6901 cactccaaat aacttcccta aggaaaaact ttctcctaat atagtgcaac caattattac
     6961 tgaaccaact aattctactg atgccacaac acctgcaact gacaactcta cacctttaga
     7021 tatacctgct gcataaaaaa tatatgcaag tgcagctgga acaatgccta acattagcat
     7081 acaagataaa acatctaaat ccttagcata atttaagatt tcccaaggtc ttgaagaagg
     7141 aatcataaat atagctccaa ataagaaact gtatactaat attgttgaac ttgaaaattc
     7201 ctttaaagca tttttactaa tagttggcat aagtgcatat gctattgctg ataatactcc
     7261 aagtaaaagc ccaaaagcgt ttaaaccttg aaaatccaaa cgtccaccag tcactgccat
     7321 aattgcacca ataaaacaaa gaataagtga aaataatttg cttctagtaa tattttcttt
     7381 ataacataat tttgaaaata ttgctaaaaa cagtggtgat gtatatagta aaactgctgc
     7441 aattgataca cccgcaatgt caattgcttt aaaataacat aaattaaaca ttgcctgaca
     7501 tataatacct attattactg aatatataat tccttttttg tttatcttta agatttgtgg
     7561 tgtccttata gatgaatata cgcctaaaac tataaatcca aaaaacaatc ttgtaaatgc
     7621 tacttgttct gatgtcagtc catttcccat taaaaacttt ccaaataatc caagtgtagc
     7681 ccataataac cctgctattg ctataaatac atacccttta cttttttctc ccattttaaa
     7741 ttcccttcta tttttattta gtcaatttac actcttcttt ttaagcaaaa actatgccat
     7801 cttaaatcaa tatatatcga ctctaaaacc ctagtctttc caataatact aataaatttt
     7861 caattttttt atatttattt ttcgcttact ttaagttgat ttagacttat tttaattgta
     7921 aaatgagtta tatataactt atttaaagtt aaaatagact gaaacttata tataaatatc
     7981 aaattttaag tatattaatc aattaaacct attttaatat atcattaaaa atttaggagg
     8041 aaaatataaa aatgaattta gatttggaat tttatcaaaa aatattagaa gcttcccatg
     8101 atgaaatatg tgtttctgat gataaaggaa taataatata ttgtaataaa gcatttgaag
     8161 aaaattatgg tctaaaaaaa gaagatattt taggaaaaaa tgtttctttt ttagaagact
     8221 ctggatattc aacaaaaagc cccataccaa ttgtattaaa aacaaagact aaattttcac
     8281 tggaacaaga tactcaaaca gggaaaaagc ttatcataac tgctacccct atatttgatg
     8341 aaaatggaca cttagaattt actgttgaaa attgtagaga tataactgaa cttaataata
     8401 taaaaaataa acttgaagac acaaaaaagc aagtaaaaaa atataaatct gaagtagaaa
     8461 cactatacag aactgcactt agaattgaag atactgtaat tatggacgga atagttatga
     8521 gaccaatcat aaacacagta aatcatgttt ctaaaacgga tgtaagtgta cttttattgg
     8581 gtgaatctgg aacaggaaaa agctcacttg ctagatatat acaccacaac agcaatagat
     8641 ctaatggccc atttataaca ataaattgtg caactatatc tccacaactt cttgaatctg
     8701 aactttttgg atatacttca ggtgctttta caggtgcaag tactaaaggt aaagttgggc
     8761 tcgtagaatt agctaatgga ggaactcttt ttttagatga aataggagat ataccacaaa
     8821 atctacaggc aaaatttttg caattaatac aggatagaac ttttactcca gttgggagct
     8881 taaaaaataa aaaggtcgat ataagaatta tatctgctac aaatgttgat ttagtttcaa
     8941 aagtaaaaga aaaaaagttt agagaagatt tatactatag attaaatgtt atagaaataa
     9001 aattgcctcc tcttagagaa agaagggata atctagtgga aattataaaa tattatttta
     9061 ataggtactc aagtgacttc aatctaaata aaactatttc taaagaagct atggatgcta
     9121 ttgcaaatta tagattccca ggcaatataa gagaattaca aaatataatt caaaaaatct
     9181 tgcttacttg tacagataat catataacaa tagatgactt accaaacatt cttacaaaaa
     9241 atatacatat taccaataat ggaaataaaa cacatatatc ccaaattaat aaggttatta
     9301 tatctgactc aaaatctact aattataaaa ataaaaattt tgacacttta attaaagagt
     9361 atgaaaaaaa tataattttg gatgcttatg aaaaatttgg tagttcttat aaagtagcca
     9421 aacaccttga aatctcacag tctaaagcca ataggttaat tagaaagtat actaatactt
     9481 aattttaaat ttgataaata aaaacttaac actatttaca tattgtaaat ttggccattt
     9541 actgttatta tgaatatggt tgaaattatt taaataaata ctgttgactt agtttcatat
     9601 atttaccatt aaacttttat cataaatatc atatcataat aatatattta ataagtctaa
     9661 taaactatac gaggtgtaaa tatgttaatg aatacccatt ttattatggc aaaatcaata
     9721 cttgataata tagatgaaaa taaaacattc tttataagcg aaaaaaattt tatatatggc
     9781 aatataaagc ctgatgcttt ttctaaatat aaattaaaaa aacattattt agatgagtca
     9841 tttaatatga ttgtttctaa aataaactat ctatgcagtc ttaatataga tagtctttct
     9901 aaatactttt cagtaagtag acttagtcag gaactaggag tcatatgtca ttttttatgt
     9961 gattttttct gtgtagctca cagtgaacgt tgggagttta agcatagctt aaataaacat
    10021 gttacttatg aaagagaact tggagcattt gcaaaagatg ttgatttatc taaaattaaa
    10081 tgtggtacta taaatagtag ttttgagaag ttttttacta aactttatag tgattacaaa
    10141 aagaaaactg attatatgaa tgatttaaaa ttttcagctt atacttgtaa tagtgtcata
    10201 aattatatac ttgactctat tttatctaat acaataaaat catatcatat agcaaattgc
    10261 ggttaactta agaattttta aacttataat tgaatataat taaaatgact gctctataga
    10321 atataatttt atacttctct aggacagtca ttttttatac taactattta tctccattat
    10381 aacatctatg gcacttttta cactatctac tacataattt gcactttgct ttacatcttc
    10441 ccttgcctta gacattgcaa aactatattt aaatccttct aacatgcaaa tatcatttgg
    10501 agaatcccca atagtagcaa cttcatctgg acttacattt aacgttttta ttacatgctc
    10561 tatacttcct ctttttgaca cacttttagg aactatattt aaagtatatg ttccagaaaa
    10621 atatatttct aagacttcac taaactcact ttttagataa agttctattc tggataactc
    10681 ttccaaattt tcagaacata cgcctatatt tcctataaga ttatcaaaac tacttctttt
    10741 ttcaaaatct gggtcaacta taaagtcact agaataatgt ctaaaagcag tggtatcact
    10801 acccacaatt acatcaccct tatatttaaa ataaagttgt gcatcacttg actcaaatct
    10861 atcaatgact ttttttacta aattatcatc tatcgttctt ttaaaaatta cattataatc
    10921 tttatcatat ataaatgaac cattttcaca tacatagtat ccattcatat ctaaactatc
    10981 tttgataata ttatgtgctt gtttaaaaat tctacctgta gcaacattaa actgtactcc
    11041 ctttttttct atttcttcaa ttgctactat atcttctttt gttatggttc cattttcata
    11101 tagagttcca tctaaatcac aaaatatatg ttttatcata aattaaaagc ctccaaaaac
    11161 aattttatat tttactaata ctttaatcaa aataacttaa agcttttcta attgtattct
    11221 caattcatta agcaaatctc tagtagctct taatcttatc tttcttctat ccccattaaa
    11281 tatatttttc tttactattg tttttccatt tatatatatt gcagtataaa ccagtcccac
    11341 aggcttctct ttggttcctc caccaggtcc agctactcca gttgtggata tacctatatt
    11401 agctttaagt cctttagcaa cgccctcagc catttctata gctgtttgct cacttactgc
    11461 tccaaactct tccaaagttt ctctttttac tcctaatctt ttcattttag aatcattaga
    11521 atatgttaca caaccttcaa gaaatacaga ggatatacct ggatagttta taagagatga
    11581 agaaaccaaa cctccagtac aagattcagc cactgctatt gttagatttt tttctacaag
    11641 tagcttagaa acagcatctt ctaaagcagt ctccccttca gatatggaaa tatctccttc
    11701 agtatataca tatttaccaa ctctactttt tattttttct tctactggtt ttattaattt
    11761 aaaagcttct ctttcatttt cagcctttgc agttattctt attgtaactt ctaattcttt
    11821 tgcatataaa gcaactgttg gattagtctg ctcattaatt atatcgagaa tttctaattc
    11881 tagatttgac tcacctattc catataatct taaagtctta gatactagca tttcatttgt
    11941 gaattgttgt aagtaaggtt tgacactctc attaaacata gctttcattt ctcttggagg
    12001 tccaggcaat actattataa atttaccatc tttttttaaa attgcccctg gagctgtacc
    12061 attattattt tttagtacta tagcatcttt tggaaagtac acttgtttca tattattttc
    12121 ggccattttt ttccctgttt taacaaagaa agattccaaa acctctagtg aaggtttatg
    12181 tagttctaat ttctgaccaa agtattctgc agcagtttct tttgtcatat catcaccagt
    12241 tggcccaaga ccacctgtag taattacaaa atcacttctc tttaagcttt catcaaaaca
    12301 ttctaaaagt ctttgtttgt tatctccaac agtactctga tggtatactt ctattcccaa
    12361 tgatgcaagt tctttagcta aaaattgaga atttgtattt actatatctc ccaataatat
    12421 ttctgttcct acacttatta tttcagcttt caaaatttat cactccttga tttatagcca
    12481 ttaagactag tcttaagttc ataacaataa ttttagcaaa aataataaca taaataaagt
    12541 taaaaaatca tttaaacata tctttaaact agattattat tctaaaacca cacataatat
    12601 aaacttcgta tatagtggta ctttcatcct caactgttaa ttcatataat taagtatgta
    12661 aataaaaagt ttactttaaa ataataaaaa ttaaaatagg agtgtataat atgaaaatag
    12721 cagttataat gggtggaata tcatcagaga gagaagtttc tttaaattca ggaaaagaga
    12781 tatataataa ccttgataaa aataaatatg aagttgtaaa agtaataata gatgataaaa
    12841 aagatatatt tacaaagata ccagaagata tagattttgc aatacttgct ttacatggaa
    12901 aatttggtga agatggatgt atacaatcaa tacttgagac tatggatata ccatactctg
    12961 gatgtggacc tctttgcagt gggatgtgta tggataaaaa tataactaaa aaaatgctta
    13021 gagattcaaa cctacctact gctccatggg ttttagtaaa atcagttgat gaaatagact
    13081 atgatgaaat agataatata ggttatccag tatttataaa accaaactca ggaggttcaa
    13141 gtgtagccac attctttatt cattctaagg atgaagttga agaagcagtt agaaaaggtc
    13201 tggaagttga tgaatttgta atgattgaaa aatatatacc aggtggagaa tatacttcat
    13261 tcatattaaa tggagaagtg ttccctacaa tatctataaa atctgattca ggattctttg
    13321 attatgaagc taaatactca gttgaaaaag gtgcaaagga agaagttgtt tacttagatg
    13381 aagaacttca aaaaagagta aacgaaattt ctgagacttg ttggaaaatt tttaattgca
    13441 aagcatatgt aagagttgat atgattataa gtgaaggtat tccatatgtt cttgaactaa
    13501 atactcttcc tggaatgact caaactagtt taattcctag aagtgctgct gctagaggta
    13561 ttaagtacag tgaactatta gataaactta tagaatactc tttaaattag atgaaataat
    13621 ttgaggtggt aatttgatat tttcaaatct aaaattaaat gataatgaac caatttatat
    13681 acaacttaaa aattatataa gtgatatgat atcaaaagga ctaattccag acaatagtaa
    13741 gctcccttct acaagggagc ttagtcagct tttacaagtt agccgaaatt ctgtagtttt
    13801 aacttatgaa gaattaaaat cagaaggatt aatctactct atttcaggaa aaggtacatt
    13861 tgttaaatca aagaataaat cttcaaatac tacatggtca ttaaactggg attgtttaga
    13921 gaatacatac tctaaaaaag ccaatgaatt ggacattata aaaagtgaaa ttccttggag
    13981 ctctgacctg atttccttta aaagtatttc tccagatgga gatttattcg acatggaaga
    14041 attaaaaaaa tcctttttaa atagaatttc actagaagga cataaattac tcaactatgg
    14101 atatgcacaa ggttataagc cacttattga ctatttacta gaatatatga caaacaaagg
    14161 tgctgatata agtaataaag atatactaat aacaaatgga ttcacagaag gctttgatat
    14221 tattatgtca tcttttaccc aagaaaaaga ctacataata tgtgaaaatc ctactcataa
    14281 tactgctata aaaattatga aatctcataa cctcaatatc ttaggtgtag atataaatga
    14341 agatggtttg gacttcaata tgctagaaga caatttaatt aagtataaaa ataaaataaa
    14401 gtttgcatac attacaccct cttatcacaa tcctacagga attgtaatga ctcctgaaaa
    14461 taggtacaaa ttctataatc ttatgaaaaa atacaatata gcaattatag aagatggatt
    14521 taatgaagaa ttactctaca atagttctca tatatttcct atctgttccc tagataatat
    14581 gaataatggg gtcgtatata ttggtagttt ttcaaaaatt ttatttcctg gaatgagaat
    14641 tggatggata tttgcagata aaaaagtaat caataagcta gaaagtgtaa aaaggtgcag
    14701 aaacatacat gtatcctttt tagaccaagg aattttattt gattatttaa gtaatggagg
    14761 ttttgaaaaa tacattaaaa aaatacgtaa attctatggg gacaaattta actttgcata
    14821 taaatgtata aaaaaatata taaaaacaga atatatcttg ggtgatggtg gtttgcatat
    14881 atttataaaa ctaaaaaata tagatactag agttttatta gaaaaatgtt atgaaaaaaa
    14941 tgttatattt atgcctggag acttattttt tacagataac tctggttttg atactttaag
    15001 actcggattt tctaggttgt cttttgaaga cattgaaatt ggaataaaaa taattggaga
    15061 aactataaat gaaatgttct aggttaacta aatattgtta gcctagttta tttttatgag
    15121 gtataatata tatagtttat atgaatctaa tactggggga taatatgagt aaaaagtcaa
    15181 aacgaagaaa aattaataaa ttaaaattaa cgattgtaat aattgtatta tttcttgctt
    15241 tattatctat atatgagata ctgttctcta atcccaacaa aaaaggtaat ataaaaagtc
    15301 aagaaactat gcaaaataaa aataatacaa cagatgattc taaaaataat caaagctcta
    15361 ccaaaaagca ggaagataat aatatcaagc ctactttttc atattcttca ataccagata
    15421 atataaaaaa taaaatgatt ggtaaatcta tgcctactga tgaacctata agctttgata
    15481 gtctatctta tttaaaactt acatattatg gatttgatga aaaaactcat caaggagaaa
    15541 tgatagtaaa cagtgaacta gctccagaag tagtggatat ttttaaggaa ttatacgaaa
    15601 aaaaatatcc aattgaaaaa attaagttaa tagatgacta tgatgctgta gatgaaaaat
    15661 ctatgtctga taataatacc tcatcatttt gttatagaac tatagctggt acaaatgtag
    15721 tttcaaatca tggtaaagga cgtgctatag atataaaccc attacagaat cctcaagttt
    15781 ctggaaatga tgttacacct aaagtaagta cagtttatgc tgataggtct agcacaaaat
    15841 ttggaatgat aaaaaaaggt gatgattgtt ataatgcttt tgtaagcaga ggttggagtt
    15901 ggggtggcta ttggaaaaac ccagattatc aacattttga aaaataatag attagaaata
    15961 aaggtgaatt atatgaattt aatgacattg gaaaatataa gtaaaagcta ttctgaaaaa
    16021 aaattactag aaaatatatc tcttggaata aatgaagggg aaaaaatagg acttattggt
    16081 gtaaatggta ctggtaaatc taccttactc aaaattattg caggagttga agaaagtgag
    16141 actggtaata taacaaaaac taatggtatt aggatagaat atctacctca aaatccaact
    16201 tacaatgaaa attcaactgt acttgaacaa gtatttaatg gaacttctga agaattaaag
    16261 ctacttggtg aatatcaaga aatcctagaa aaacttaatt caaactttac agaggaatta
    16321 aataaaaaat tattatcagt acacgaaaaa atagatgcct taaatctttg ggatttagaa
    16381 agtgaagcta agtcagtact tacaaagctt ggtataaatg actttaatca aaaaatagca
    16441 gaactttctg gtgggcaaag aaagagagtc tctctggcat cagcccttat tacaccttgt
    16501 gagtttttga tactagatga gcctacaaac catctagaca atgatactat agattggctt
    16561 gaggaatatc ttaattccag aaaaggttca ttgcttatga taactcatga tagatacttt
    16621 ttagaccgtg ttacaaatag aattattgaa ctggacaagg gtagattatt cagttatgat
    16681 gggaattatt caatattttt ggaaaagaaa atggaaagac tttcgctaga agcaagtatt
    16741 gaagataaaa gacaaaattt aataagaaca gagcttgcgt gggtaagacg aggagctcag
    16801 gctcgtagca ctaaacaaaa agctcgttta caaagatttg atgaacttat aaatagagat
    16861 tcttttaaac ctgatgaaaa tcttgatata tctgttgcat caagtagact tggaaataaa
    16921 ataatagaaa tacacaatat atctaaatca tttgaagaaa atactgttat agataattta
    16981 gaatacactt taactcgtac agatagaatt ggtataatag gcaaaaatgg tatgggtaaa
    17041 tctaccctta taaatatact taatggagaa atagagcctg atagtggata tatttcaatt
    17101 ggagaaacgg ttaaaatagg ttgtttttct caagacgact ctcatatgaa tattgatatg
    17161 agggctatag actatgtaaa agaagctagt gactatatag aaacttcaga tggtactagg
    17221 attacagctt ctcaaatgtg tgaaagattc ctttttaatg gtactatgca atatactttg
    17281 ataggtaaat tatctggtgg tgaaagaaga agactacatc ttcttaaaat ccttatgaca
    17341 gctcctaatg tgcttctttt agatgaacct acaaatgatt tagatattga aacgcttaaa
    17401 atattagaag aatacctaga cgaatttaaa ggtgtagtag ttacagtatc acatgataga
    17461 tatttcttag atagaatttg taataaaata tttgcatatg aaggtaatgg aaaaattcat
    17521 atctatacag gtaattacag tgattatctt atttatagag agattcaagg aattgaattt
    17581 gaagaatcta aaaaggaatc agttaaaaaa gatgactcta ctaccccaaa aaaagaaaaa
    17641 cctaaaaatg ataaaaagct taagttctca tataatgaac aaagggagtt tgaaaatatt
    17701 gactctgata tagaaaaact cgaatctaaa attgcaacac ttgatgaaag tactgcaaaa
    17761 ttttcaactg attttactaa acttcaagaa attttagatg agaaatctaa actagaaaaa
    17821 gaacttgaat acaaatatga aagatgggaa tatttaaata atctagctga tgaaatagct
    17881 aataataagt gatattaaaa taatcttaag tttaataagt tttgctttaa atctgtactt
    17941 tataaacatt ttaaaggaag tgtctcaaaa tgaacttttt agttttatga gacactcttt
    18001 tttatgagaa atcaaatatg ttttcatcat ttcaacaata caaaagaggc ttatctctat
    18061 tttgagacag cctctttcat aattaccttc taatttatat gctctaatat atctataata
    18121 attcttatta tatctatata tccagtcttt caatatagtg acaattaact tcaatatgat
    18181 ttaaaaatac taacaacctt tcataaagtg gatgagattc ttctccatat ttagcttcta
    18241 ggttcttgcc tatttctcta acagtccttt gaccatcaat ttgtaaaaaa acaaaactgg
    18301 aatattcatc tagttctatt tttttgtatt taggtattct agcttttaac tttctaaaaa
    18361 acttttgaat tttatggtcc tgtttttcaa gtatagtaac aacactattt tcagctactt
    18421 catattcaag tccatcagta attttaaata caatatctaa aacatcatta ctcttgctca
    18481 ttatttttta ctttagataa aacaataggt aatgctgtaa gcacaactaa aactactaat
    18541 aaagcaattg ccattgaatt tgttgcagca aatccacttg gtacctttgg agttacaact
    18601 ccagttactt gtaatattat acctattaaa ccaatgatag aaccaccagc aacaagacca
    18661 gaagataaac ttataccatt agaaacttta acttcttttt ctttttcagt tttagatact
    18721 aattcaacaa ataaacgaat taatgctcca actaatatta tagaagttgt tgcaattggt
    18781 aaatagaatc ctatagctat agtcattatt ggtaaattta atgcatatag aactattgcc
    18841 ataaatactc caacaataat cataacccaa ggtaaactac ctgacataat accagaagtt
    18901 aatgttgaca ttaagtttgc ttgtggtaat gcaaattgta cattatcacc agttgctcta
    18961 agttggtcag aaagtaataa aattactcct acaacaacta taacaccaac tatacttgca
    19021 attgtaaagt aacgttgcat ttcgtttttg ctaccaccaa taatataagt tactttttga
    19081 gattgagtat aacctccagc tatagcaata gcaacaacta taaatgaacc aaataaaagt
    19141 aatgatttat tagcttctaa atcagtccat cccattataa caaacactaa tgttacaata
    19201 actagagaag ctatagtcat accagaaact ggaagatttg atgttccgat tgttcctgtt
    19261 aaacgaccag ctactatgac aaataataaa gataataaaa gagatatgat agctccaata
    19321 attgccataa caatgttacc agaaattaag aatgctgcta aaaatccaat aactactcca
    19381 cctaataaaa gtatcatttg tatagatgaa ccttcttctc cctctccagc acttgattta
    19441 gctttaagtg tttcttttat agaagcgatt atagttggta taagttttat tgcaccaata
    19501 atacctccac aaagcatcat accagcacct atatatttta cataactact agatattgct
    19561 ccaacatcca tttggttaag aggcattgca ggattattcc ataccatagc tccatcttta
    19621 gccatatctg taaagtatcc tattaaaggt gctataccaa agtttgataa aatacttcct
    19681 gcaaacattg ttaaagaaac ttcaagccct actataaatc caattcctaa aagtagagga
    19741 tttacttcaa tatcaaattt ccatttgtag aatttgcttc ctacaagact cattacattg
    19801 tttgctacat ttaagaatga accagttaat acagtaataa atccacttat tacaaatcca
    19861 attcccatgt acttaattga atctccacca gcctctgaag caacaagtgt ctcagatata
    19921 gccattgatt ctgggtacat aagtttacca tgttcttcaa ctattagata gttgtgaact
    19981 atagcagcaa ttcctatacc aaataaaact ccaccaactc caacagctaa gccttcaaag
    20041 aatgtaattt ggcttccaat taatataact gctggtaata cgaaaataaa tccacttgca
    20101 acagattctc caccacttga cataccttgg attaagtttt taccaagaat accctttgaa
    20161 cgagcaaatg ctccaacaaa agcagaacct attatagctc ctggaatacc tgcggcaact
    20221 gtaagaccgg ctttcattcc agagtaagtt gtagatgcgg caaaaatggc agctaaaata
    20281 ataccgataa tcagtacagc tacatttcca ccggttctgg acttatctgt gatatatgga
    20341 acgtaatctt ttccactaac ctcaccataa gcacccttag gtaatttttt gttcataaaa
    20401 ttttcacccc ttttcaattt gtatatatta taacatatat acatataaaa cttaactttt
    20461 tattatttta caaatagcga gtc
//
LOCUS       DBGJFF010000065        20314 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.066, whole genome shotgun sequence.
ACCESSION   DBGJFF010000065 DBGJFF010000000
VERSION     DBGJFF010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20314
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.066"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            <1..537
                     /locus_tag="ACOIAF_002350"
     CDS             <1..537
                     /locus_tag="ACOIAF_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HGZ2669359.1"
                     /translation="SCGDFIENPKYLKKSLNKLAKLQRELSRKTIGSLNRNKARLKVA
                     GLQEHIANQRNDFLQKLSTKLIKENDIICIEDLQVKNMIRNRKLSRLISDVSWSEFIR
                     QLEYKANWYGRKIVKVGKFFASSQICNKCGYKNEEVKDLNIREWICPSCNETHDRDIN
                     ASINILKEGLRLITIQNK"
     gene            655..960
                     /locus_tag="ACOIAF_002351"
                     /pseudo
     CDS             655..960
                     /locus_tag="ACOIAF_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438308.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
     gene            973..1878
                     /gene="truB"
                     /locus_tag="ACOIAF_002352"
     CDS             973..1878
                     /gene="truB"
                     /locus_tag="ACOIAF_002352"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HGZ2669360.1"
                     /translation="MNKIISILKPTGMTSHDVVSRVRKILNIKKVGHTGTLDPDASGV
                     LPICIGKATKVCEVILNKDKSYICELTLGISTDTYDASGEILKKVDDFKFSNEDIERA
                     FDTQRGEINQLPPIYSALKVNGKRMCDLVRSGRQSEITLKTRRVNIKDIKILSIKGNK
                     VMFYVECSKGTYVRSICHDIGEYLGCGAHMSFLNRTSSGKFDLDNSITLEELELFYEN
                     KTLDKYLYDIDYVLDSFNYVVLNPNAIKYYSNGGSIDDKRFLKNNFDKDDEFVRVYST
                     DNFLGLGKLSKHNNTISVKSDKMFI"
     gene            2009..2938
                     /locus_tag="ACOIAF_002353"
     CDS             2009..2938
                     /locus_tag="ACOIAF_002353"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HGZ2669361.1"
                     /translation="MVIIKSIKEIANIKESVITIGNFDGVHKGHQVLIGKTVEHAKKE
                     NIQSIVFTFANHPVNYFRPNSTKNIISNDDKIKLLEDLGIDIVVIIPFDEYMTKIPAK
                     DFVDEILVKKLKAKKIVIGHDFTFARAKEGNVNLLKSLEHEFGFEVEVIKPIKINDVR
                     VSSTYIRSLVSQGDMANVKEYLGRNYKLEGCVIHSKHLGRTIGFPTANIDLKNNMLVP
                     KRGIYASIVHIGDETYFGATNVGYNPTVNGKTLSIETNILEFDRDIYGENIIVEFLER
                     IRDERKFNSIDDLKNQLYNDTNYVYENYVCKNK"
     gene            3042..3299
                     /gene="rpsO"
                     /locus_tag="ACOIAF_002354"
     CDS             3042..3299
                     /gene="rpsO"
                     /locus_tag="ACOIAF_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HGZ2669362.1"
                     /translation="MINKSEIIKEYGRAEGDTGSPEVQIALLTARINELNGHLKTHKK
                     DHHSRRGLLKMVGRRRNLLAYLKEKDLEGYRALIAKLGLRK"
     gene            3539..4384
                     /locus_tag="ACOIAF_002355"
     CDS             3539..4384
                     /locus_tag="ACOIAF_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HGZ2669363.1"
                     /translation="MKIKLVCDSLCDIPDEISEKDYIEIVPLTVIFNDREYIEGVDIK
                     KEEFYKKVKEIKQIPKTSQATYMEFKEVFDKFITEGYHIICMTGAANASGTFQSAMIA
                     KNDVNENEKIHIFDTRNLSLGSGQYVIKACELLEEGLGFEEIIDELENTRSSVRLLFA
                     PYTLDFLKQSGRVPVATALIGNMLNIKPIFFFDNGEAKLVNKVRGIKNIASKLVDIIL
                     EMNEGSLEGKIVTIGCGDNLHDCEILKDEVNKRIKARRVLFTRGGVSICSHTGPDILA
                     ISCSN"
     gene            4509..6620
                     /gene="pnp"
                     /locus_tag="ACOIAF_002356"
     CDS             4509..6620
                     /gene="pnp"
                     /locus_tag="ACOIAF_002356"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HGZ2669364.1"
                     /translation="MFEHKIFKMDFAGRELSVEIGKICEMASGSCIVRYSDSMVMVNT
                     TKSAKPRDGIDFFPLSVDYEEKLYSVGKIPGGFLKREGKPSEKAILTSRLIDRPIRPL
                     FPKGFRNDVQVVATVLSVDQDCTPDIVAMIGSSIALSISDIPFNGPTGSVCVGLVDGA
                     FVVNPNAEQREKSSMHLVVSGTKEAIMMVEAGADEVPDEVMLDAILFAHQEIKKIVEF
                     IEGIVAEVGKEKMPVELYHAGEEITQLVREFATDKMKKAVQTFEKLERMENMDRVKEE
                     TLAHFEETLEDFEDFVGDIEEVLQDIIKEEVRKLIVHENVRPDNRKLEEIRPIWCETG
                     MIPRAHGSAIFTRGQTQVLNVATLGALGDVQKLDGLDEEENKRYMHHYNFPAYSVGEA
                     RPSRGPGRREIGHGALAERALLPVIPSQEEFPYAIRLVSEVLSSNGSTSQASVCGSTL
                     SLLDAGVPIKDMVAGIAMGLIKHDGKVAVLSDIQGMEDHLGDMDFKVAGTEYGITAIQ
                     MDIKIDGIDKEILQRALKQAKEGRIHILGEMRKTISQPKPELSPYAPKIVKMQINPDK
                     IKDVIGPGGKIITKIIDETGVKIDIEQTGEVFISGIEIDMIKKAQELINNIVVEPEVG
                     KTYKGKVSRIMNFGAFVEILPGKEGLLHISHIAHERVAKVEDVLNIGDEVEVKVTEID
                     EKGRVNLSRKVLLPKPEHKNK"
     gene            6886..7629
                     /locus_tag="ACOIAF_002357"
     CDS             6886..7629
                     /locus_tag="ACOIAF_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HGZ2669365.1"
                     /translation="MIMMVLNKSKLKKIIILILVVLLVLSGGVMVLKLFNNKPVFNLF
                     NGVDLPYERGTKDKSGYVALTCNIDLGWETEYVESILETLKKENVKITFNVTGKWAEK
                     NKDELLKIKKQGHEIGNHGYKHLDYSTLSYEDNYEQIETSKKIIEEIIGEKTKFFQAP
                     AGSFGPETVKAAKALGYTSIKWDADTIDWKYKDQPEVIIDRMKKKDIKDSSIILMHPT
                     NATTKCIDDIIAIVREKGLKPGKLSDVFK"
     gene            7787..9034
                     /locus_tag="ACOIAF_002358"
     CDS             7787..9034
                     /locus_tag="ACOIAF_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HGZ2669366.1"
                     /translation="MYKTKILENGLTIIGEEIPYLKSITLGIWINAGSRIEEAQVSGT
                     SHFIEHMMFKGTKNRTSKEIASSIDNLGGQINAFTSKECTCYYVKLIDEHIDTGIDVL
                     SDMILNSKFDKNDIDKERLIILEELKMYEDSPDDLSYDLLVENIYANDGLGMNIIGTK
                     ESLYNITRESMLEYLNKYYIPNNAVISIAGNFNFDDMVEKIKSKFGHWEKKNLSIDIS
                     EAKFNPCFISKNKDTEQVNLAMCLKGIPFENDEEVYSMAVVNNIFGGSISSRLFQKIR
                     EEKGLVYSIYSSQTLYRKCGELGIFASMSTENLQDVYNLIKKEIENIRENYLTEKEIS
                     ESKEQLKGNYILDLESTSSRMMSTGKSMLLSKKVKTTDEILECINNVNINSIKKVVDK
                     VFNIENIGTCIVGRDVEKILHLD"
     gene            9069..9314
                     /locus_tag="ACOIAF_002359"
     CDS             9069..9314
                     /locus_tag="ACOIAF_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HGZ2669367.1"
                     /translation="MNLSEIAGKEIVNLVTGERLGVIGECDLILDETTGGILALLIPK
                     EKGFFARRKDKSFLEVPWRNVKKIGNDMIIIEYEGYI"
     gene            9324..10547
                     /gene="dapG"
                     /locus_tag="ACOIAF_002360"
     CDS             9324..10547
                     /gene="dapG"
                     /locus_tag="ACOIAF_002360"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HGZ2669368.1"
                     /translation="MSILVQKFGGTSVESYEKMNEVCKIVKAYKKNDEELQLVLVVSA
                     MGRKGAPYATDTLINLCSAVNDEPSKRELDLIMSCGEIISGTILANLLNAQGIDSVFF
                     TGQQAGIITSDEYSNAKIKYINPKKVKRALDDGKVVVIAGFQGVTDDGEITTLGRGGS
                     DTSAVAIGKALECETVEIYTDVDGIMTADPRVEPNAKVLSFIDYEEVFQMADKGAKVI
                     HPRAVQLAKSGNITLAIKNTMNPTFEGTKIGSLCRHLEDNIEYEQERDFKVAVANKDS
                     VAQVKIKSAEEVFTEVLNEIEKKLITIDMINFFISEKAFVVEDADIKSLKEILDKFEL
                     DYEVNRDCAKVTLICSRIADEMSGIMSKVVRGLSKAGVSLLQTSDSNMTISCLVSEED
                     MHTAVHAIHQQFYLK"
     gene            10660..11394
                     /locus_tag="ACOIAF_002361"
     CDS             10660..11394
                     /locus_tag="ACOIAF_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HGZ2669369.1"
                     /translation="MKDYREDNERFDKDNCSEESSCEERECIKQFGTNEMPPQPPKDI
                     QCITIIGEIEGHFIGNPQKKATKYEHIIPMLYSIEESNDVKGVLVVLNTVGGDIEAGL
                     AIAELLNSTSKKVVTLVLGGSHSIGVPLATAGDYSFIAPTATMIIHPVRTTGLVIGIN
                     ETFEYFKKMQDRIIQFIIRTSNIKKDVLEKLMHEKDELVSDVGSVLIGKEAVDYGLID
                     EVGGLKEALKKLRELIKESEEEKNND"
     gene            11517..13928
                     /locus_tag="ACOIAF_002362"
     CDS             11517..13928
                     /locus_tag="ACOIAF_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HGZ2669370.1"
                     /translation="MATKKKKKKKVNTLSFNAEYHNLITIFIGVFLLYSLNSNSMGWI
                     PVLMQNLFKGLFGGLSIAIPFIVIITGLLGFFDGNEYIYRLRKTKLYYIIILFIFVFY
                     GLLNAGTLPVDSPLKGNMFDDVMKLGVSGQGSGLIATTIAYYMSKIFGIAGGWLISIF
                     ALILSVMFIFNISIKDLVSNAKSKSKASKDSNLTFKDKIANMKKSAIDMMTDEVDDTT
                     INKKPGFFKGLMSKGLNNDDEEDEYFEEENSDGVDDKTIKIVGFNKAEDEYLEILEGT
                     QSMPELDVLKELQKATNENPVVDTKPEKKVDIAKPNLNIEKTQPMSIVAEPVNEDYSN
                     YKKPSIELLNKVNKKSDENGKKKVLKNASLLEKTLSDFGVEAKINQVTVGPTITRYEI
                     QPSPGVKVSKIVNLTDDIALSLAAKSIRIEAPIPGKSAIGIEVPNEEAQMVGVREVLE
                     SEEFNNFNSPLAMGLGKDVAGKIIIGDIGKMPHLLIAGSTGSGKSVCVNTLISSILYK
                     ANPDEVKLLLIDPKVVELANYNGIPHLLIPVVTDPKKAANALNWAVTEMNRRYKLFAD
                     AQVKDVTSYNEKAEEKLPKIVIIIDELADLMMASANDVEDYICRLAQMARAAGMHLIV
                     ATQRPSVDVITGVIKANIPSRIAFAVSSQTDSRTILDMGGAEKLLGKGDMLFYPLGAA
                     KPVRLQGAFISESESEKVIDFVKSQVKDGIKYEEDIIETISKVNTSKGSDEDEFLSEA
                     IEFVVESGQASASMLQRRFKIGFNRAARLIDSMEERGIIGASEGSKPRKVLISKQDLQ
                     NLEGE"
     gene            13931..14989
                     /gene="mnmH"
                     /locus_tag="ACOIAF_002363"
     CDS             13931..14989
                     /gene="mnmH"
                     /locus_tag="ACOIAF_002363"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HGZ2669371.1"
                     /translation="MSVIKIEKALKLDKVIFVDVRTEGEYEEDHILNAINMPLFKNNE
                     HNEVGTIYKMQGKHEAIQKGFDYVSYKLKDIYLQAAELALNYDNIVIYCARGGMRSGS
                     IVNLLSSLGVNVYQLEGGYKAYRNFVLEYLRHVMDTKNFVSVHGLTGTGKTDLLHLLD
                     EKGIDVLDLEGIAKNSGSTFGFITFDKKPPSQKNFETKLFESIFFSKENYIFVESESK
                     RVGSVTVPNEIFEAMTREGHHILLECDIENRVDRLCRDYIYDKDEGNILILKECINKF
                     RKRLGNKVVDDYIDLLESGEYKELVKRYLLEYYDPLYMHSVEKYKYSKIINFNDTQKA
                     LEDVIDLYESILEGESEC"
     gene            14983..16317
                     /gene="rimO"
                     /locus_tag="ACOIAF_002364"
     CDS             14983..16317
                     /gene="rimO"
                     /locus_tag="ACOIAF_002364"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HGZ2669372.1"
                     /translation="MLKIALESLGCSKNLVDAEIMMGILNNKGYKLIGDFEEADVIIV
                     NTCGFIESAKQESIDTIINFAELKKTGNLKLLIVTGCLAQRYSEELKTEIPEIDAIVG
                     TGSYQNIDKILKELSEMHQIVSLNDIEFVFNEDLPRYISTPSYMAYLKIGEGCSNNCT
                     YCIIPKLRGKYRSRKFEDIIKEAKKLAESGVKELVVIAQDTTKYGFDLYGKERLSELL
                     EELAKIDGFKWIRVMYSYPESITEELIQVIKKYDNICSYFDMPIQHASNNILKLMNRK
                     TTKEDILNKINLIRSNIPDAILRTTIIVGFPGETEDDFKQLVDFVEEVKFDRLGAFAY
                     SREEDTPADRLPNHIDEEVKIQRRDTLMMIQQKISEELNDKKIGKTYEVLIEEQIEDN
                     VYTGRTQGDAEEIDSIVYVKSVDNLEVGEFVSVQINDAMEYDLMGDVLYELA"
     gene            16304..16846
                     /gene="pgsA"
                     /locus_tag="ACOIAF_002365"
     CDS             16304..16846
                     /gene="pgsA"
                     /locus_tag="ACOIAF_002365"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HGZ2669373.1"
                     /translation="MNLPNKLTLFRIFLIPVFVLIMLLNVPNKFLIACIIFIIASITD
                     ALDGKIARKYNLVTDFGKFMDPLADKLLVISALTCMIEDHLVSSWMVIIIVARELTVS
                     ILRAIAAADGKVIAAGNSGKLKTITQMVSIVFLLLGAQFENVLILNIGEILILIATLL
                     TLYSGWEYLYKNKELFMSSK"
     gene            17134..18180
                     /gene="recA"
                     /locus_tag="ACOIAF_002366"
     CDS             17134..18180
                     /gene="recA"
                     /locus_tag="ACOIAF_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HGZ2669374.1"
                     /translation="MSVDQEKLKALNEALGKIEKDFGKGSVMKLGEATSMSIDVISTG
                     AIGLDIAIGIGGLPRGRIVEVYGPESSGKTTVALSCVASAQKDGGIAAFIDAEHALDP
                     VYAKALGVDVDNLIISQPDTGEQALEIAEALIRSGAIDIIVIDSVAALVPKAEIDGDM
                     GDSHVGLQARLMSQALRKLTGSIKKSNCVAIFINQLREKVGIMFGNPETTTGGRALKF
                     YSSVRLDVRKIDTIKQGDKVIGSRTRVKVVKNKVAPPFKQAEFDIMYGEGISKIGDLL
                     DIAADVDIVKKSGSWYSYNDTKLGQGRENVKKFLEDNLDLTTEIDEKVRAFYNLNEEH
                     EESGTSVSKEIVEE"
     gene            18356..19897
                     /gene="rny"
                     /locus_tag="ACOIAF_002367"
     CDS             18356..19897
                     /gene="rny"
                     /locus_tag="ACOIAF_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HGZ2669375.1"
                     /translation="MDVIDSVVMIVIGAAVGIIAGYFVRKNISEAKIGQAENLAKEII
                     DKAHHDSETVQKEKLLEAKEEIHKWRTEAERENRERRTEVQKYEKRVVQKEEVLDRKL
                     QNLESKEVNLSEKLKVVEKKEEEVEVIKTQQLEKLESISGITSDKAKEIILTNAERDV
                     RREMSIMIKEIESQAKEEADKKSREIIGYAIQKCAADHVAETTVTVVSLPNDEMKGRI
                     IGREGRNIRTLETLTGIDLIIDDTPEAVILSGFDPIRREIARIALEKLIADGRIHPAR
                     IEEMVEKARKEVDNIIKEYGEQAAFETSVHGIHPELIRLLGRLNYRTSYGQNVLKHSI
                     EVAHIAGIMAAEIGADIRLAKRAGLLHDIGKAVDHEMEGTHVEIGMDLLRRYKESKEV
                     IHAMSTHHGDYEPQTVEAVLVTAADAISAARPGARRETLEAYIKRLEKLEEIANSYEG
                     VEKSFAIQAGREIRIMVKPEAINDEEIHLLARDMTKKIEDELEYPGQIKVSIIRETRA
                     IEYAK"
BASE COUNT         8319 a         2057 c         3913 g         6025 t
ORIGIN      
        1 agttgtggag actttataga aaatcctaag tatcttaaga agtcattaaa caagcttgct
       61 aaattacaaa gagaattatc gagaaaaaca atcggtagtt taaatagaaa taaagcaaga
      121 ttaaaagttg caggacttca agaacatata gctaatcaaa gaaatgattt tctacaaaaa
      181 ctatctacta aattaataaa agagaatgat attatttgca tagaagattt acaagtaaag
      241 aatatgatta gaaatcgtaa actatctcgc ttaatttcag atgtatcatg gtcagaattt
      301 attcgtcaat tagaatacaa ggctaattgg tatggtagaa aaattgtaaa ggtaggtaaa
      361 ttctttgcaa gttcacaaat atgcaataaa tgtgggtata aaaacgagga agttaaggat
      421 ttaaatataa gagaatggat ttgtcctagt tgtaatgaaa ctcacgatag agatataaat
      481 gcaagtataa atatattaaa agaaggatta agactaataa cgattcaaaa taaataactt
      541 atgtaagaac cgtaggaact acggggatag cctgtggaga attagtaaga catattttaa
      601 tatgcaaaat tctgtgaagc aggaaccctg tgactttagt catgggaggt tcagtcaaag
      661 gtatctagta tagttttaac tcaagacatg atgaatagaa ataatattag ctttaatgat
      721 gttgatggaa taacaacata tacaagagac ataaaaggta tagaagttgg aattttattt
      781 aaagagaaaa aacaaaatga aattaaagtg agttttaggt caaaaaatta tgttgatgta
      841 agtgaaatag ccaagttatt tggcggtggt ggtcatgtaa gggctgctgg atgtactatt
      901 agagatagta ttgataatgc aaagaaaatg gtactagagg cagtgttaaa atcaatttaa
      961 ggtgacagta aaatgaataa gattataagt atattgaaac ccacaggtat gacatctcat
     1021 gatgttgtaa gtagagtgag aaagatttta aatataaaaa aagttggtca tacaggtact
     1081 cttgacccag atgcttcagg tgttttacca atctgtatag ggaaagctac aaaagtatgt
     1141 gaagttattt taaacaaaga taaatcgtat atatgtgagc ttactttagg tataagtaca
     1201 gatacttatg atgcttcagg tgaaatttta aaaaaagttg atgattttaa atttagtaat
     1261 gaagacatag aaagagcttt tgacacacaa agaggagaga taaatcaact tcctccaatt
     1321 tattcagctt taaaggtaaa tggaaagaga atgtgtgatt tagtaagaag tggtagacaa
     1381 tctgaaatta ctttaaaaac aagaagagta aatataaaag acataaaaat actaagtata
     1441 aaaggtaata aagtaatgtt ttatgttgag tgttctaaag ggacttatgt gagaagtatt
     1501 tgccatgata taggagaata tctaggttgt ggagctcata tgtcgttttt gaatagaaca
     1561 tcttctggta aatttgactt agataattca ataacattag aagaattaga attattttat
     1621 gaaaataaaa ctttagacaa atacttatat gatatagatt atgtccttga ttcgtttaat
     1681 tatgttgtat taaaccctaa tgctataaaa tattatagta atggtggctc aatagatgat
     1741 aaaaggtttt taaaaaataa tttcgataaa gatgatgaat ttgtaagagt atacagtaca
     1801 gataatttct taggattagg aaagttatca aaacacaata atacaataag tgttaaaagt
     1861 gataagatgt tcatataacc aatgtattta aaaaaacaac aaaagagata gtactataat
     1921 aacttctata gtctatctct tttacttatg ctataatagt ttgtgaataa gttaagagta
     1981 aattatattt tagcggggaa catttattat ggtgattata aaatctataa aagaaatagc
     2041 aaacataaaa gaaagtgtca ttaccatagg aaattttgat ggtgtacaca aaggacatca
     2101 agttcttata ggaaaaacag ttgaacatgc aaaaaaagaa aatatacaga gtattgtatt
     2161 tacatttgca aatcatcctg taaattactt tagaccaaac tcaacaaaaa atattataag
     2221 caatgatgat aaaataaaat tattagaaga tttgggaata gatatagttg taatcatacc
     2281 atttgatgag tatatgacta aaatacctgc aaaggatttc gtagatgaga tactagtaaa
     2341 aaaattaaaa gctaaaaaga tagtaattgg acatgacttt acatttgcaa gggcaaaaga
     2401 aggcaatgtt aatctattaa aaagcttaga acatgaattt ggttttgagg tagaagtaat
     2461 aaaacccatc aaaatcaatg atgtaagagt gagtagtact tatattagaa gtttagtatc
     2521 ccagggtgat atggctaatg tcaaggaata tttgggtaga aattacaagt tagaaggttg
     2581 tgttatacat tctaaacacc ttggaagaac tataggattc ccaacggcaa atattgacct
     2641 taagaataat atgctcgttc caaagagagg tatttatgct tctattgtac atattggaga
     2701 tgagacttat tttggtgcta caaatgtagg ctataaccca acagttaatg gaaaaacttt
     2761 gtctattgaa acaaatattt tagagtttga tagagatatt tatggggaaa atataattgt
     2821 tgaatttttg gaaagaataa gagatgaaag aaagtttaat tcaatagatg acttaaaaaa
     2881 tcaattgtat aatgatacaa attatgtgta tgaaaactat gtttgcaaaa acaagtaatg
     2941 tatgataaaa tgattaagga aaataaccat tactcggcat ttgaatactc caatcaatgc
     3001 ttagtaatag gcgtttctaa aaaaatacgg aggtaatcta aatgataaac aaatcagaaa
     3061 taataaaaga atatggaaga gctgaagggg atactggttc tccagaagta caaatagctt
     3121 tattaacagc tagaataaac gagttaaatg gacatttaaa aactcataag aaagaccacc
     3181 attcaagaag aggactatta aaaatggttg gtagaagaag aaacttatta gcttacttaa
     3241 aggaaaaaga tcttgaagga tatagagcat taatagctaa attaggatta agaaagtaat
     3301 ttcattcttt ataaaaaagc aggtatagtg tatacctgct tttttatagt taattcatta
     3361 taaagtaaaa tagttaataa taaaagtagt ttatatgtta taaaaaatta taaaatgaat
     3421 aatattgggt aagaatatta aaatagtata caaatacata atcaatttaa aaggaaagac
     3481 aggataaata tagaaatttt ataacaggat aattagttgg aataaggagg atattatttt
     3541 gaaaataaag ttagtatgcg atagtttgtg cgatattcca gatgaaatat cagaaaaaga
     3601 ttatatagag atagttccac ttacagttat ttttaatgat agggaatata tagaaggagt
     3661 agatataaag aaagaagagt tttataaaaa agtaaaagaa atcaagcaaa tacctaaaac
     3721 ttcacaagct acatatatgg agtttaaaga agtgtttgat aagttcataa cagaaggtta
     3781 tcatataatc tgtatgacag gggctgcaaa cgcatcagga acttttcaaa gtgctatgat
     3841 agctaaaaat gatgtaaatg aaaatgaaaa aatacatata tttgatacta gaaatttatc
     3901 tctaggtagt ggacaatatg ttataaaggc atgtgaactt cttgaagaag gattgggatt
     3961 tgaagaaatt atagatgaat tagaaaatac tcgcagtagt gtaagattgc tttttgcacc
     4021 atatacactt gattttttaa agcagagtgg aagagttcca gttgcaactg ctttgatagg
     4081 aaatatgtta aatataaagc ctatattttt ctttgataat ggggaagcta agttggtaaa
     4141 taaggttaga ggaataaaga atatagcaag taaattggtc gatataattt tagaaatgaa
     4201 tgaagggtct ttagaaggaa aaatcgtcac aataggttgt ggagataatt tacacgattg
     4261 tgaaatatta aaagatgaag taaataaaag aataaaagca agaagagtat tatttactcg
     4321 aggaggagtt tctatttgct ctcatacagg gccagatata ttggctataa gttgttctaa
     4381 ttagtagtaa tataaggttt ttgcatatat atgtgaagat aattttatat tgttaattta
     4441 tagtattttt gataatatag taaatagagt gatatacgtg ttttttaata agaggaggac
     4501 aagcattaat gtttgaacat aaaatattta aaatggattt tgcaggaaga gaactttctg
     4561 tagaaatagg aaagatttgt gaaatggcta gtggtagttg tatagttcga tacagtgata
     4621 gtatggtaat ggtaaataca actaaatcag ccaaacctag agacggaata gacttttttc
     4681 ctttaagtgt ggattatgaa gaaaaattat actctgtagg taagatacct ggaggttttt
     4741 taaagagaga aggcaagcct tcagagaaag ctatacttac atcaagattg atagatagac
     4801 caataagacc tttattccca aaaggattta gaaatgatgt tcaggtagtt gcaacagttc
     4861 tatcagtaga ccaagattgt acaccagata tagtagcaat gataggttct tcaatagccc
     4921 tttctatatc agacatacca tttaatggac caacaggttc agtatgtgtt ggccttgtag
     4981 atggtgcatt tgttgtaaat ccaaacgcag agcaaagaga aaaaagttca atgcatctag
     5041 ttgtttcagg tacaaaggaa gcaataatga tggttgaagc cggagctgat gaagttccag
     5101 acgaggtaat gcttgatgct attttatttg ctcaccaaga aataaaaaaa atagttgagt
     5161 ttatagaagg tatagttgca gaagttggaa aagaaaaaat gcctgttgaa ttataccatg
     5221 caggagaaga aataactcaa ttggttcgcg aatttgcaac tgataagatg aaaaaagcag
     5281 ttcagacttt tgaaaagtta gagagaatgg aaaatatgga tagagttaaa gaagaaactt
     5341 tagctcactt tgaagaaact ttagaagatt ttgaagattt tgttggagat atagaagaag
     5401 tattacaaga tataataaaa gaagaagtaa gaaaacttat agtacatgaa aatgtaagac
     5461 cagataatag aaaacttgaa gaaataagac ctatatggtg tgaaactggc atgataccta
     5521 gagctcatgg ttcagccata tttactagag gacaaactca agtacttaat gttgctactt
     5581 taggtgcttt aggagatgtt caaaaactag atgggttaga tgaagaagaa aacaagagat
     5641 atatgcatca ttataatttc cctgcatata gtgttggaga agctagacca tcaagaggac
     5701 caggaagaag agaaatagga catggtgctt tagcagaaag agctttatta ccagtaatac
     5761 catctcaaga agaatttcct tatgctatca gattagtttc tgaagtatta agttcaaatg
     5821 gttctacatc tcaagcaagt gtatgtggct caacattatc tttacttgat gcaggtgtac
     5881 ctataaaaga tatggttgca ggtatagcaa tgggacttat aaagcatgat ggtaaggttg
     5941 cagtgctttc agatatacaa ggtatggaag accatttagg cgatatggac tttaaagtag
     6001 ctggaacaga gtatggtata acagcaatac aaatggatat aaaaattgat ggtatagata
     6061 aagaaattct acaacgtgct ttaaaacaag ctaaagaggg tagaatccat atactaggag
     6121 agatgagaaa aactatatct cagccaaaac cagaactttc accatatgca cctaagattg
     6181 ttaaaatgca aataaatcct gacaagataa aagatgtaat aggacctggt ggaaagataa
     6241 taactaagat aatagacgaa actggagtta agatagatat tgaacaaact ggggaagtat
     6301 ttatatcagg tattgagatt gatatgatta agaaagctca agaacttata aataatatag
     6361 tagttgaacc agaagtagga aaaacttata aaggtaaggt aagtagaata atgaactttg
     6421 gagcatttgt tgagatactt ccaggaaaag aaggattact tcatatatct catatagctc
     6481 atgaaagagt tgccaaagtt gaagatgtat tgaatatagg ggatgaagtt gaagttaaag
     6541 ttacagagat tgatgaaaaa ggaagagtta atttatcaag aaaagttctt ttaccaaaac
     6601 cagaacataa aaataaataa cttattatat aattaaaagc aaagtcttag gattttgctt
     6661 ttttaaatac aaaaaagtag ataattttaa aatgatagtt atggatggct tatagttctt
     6721 tttctgtcag gctttactct tataaagaag ctatttaaaa gctgattaat agagagcttt
     6781 attgatatat atagaatcac ttaagttata tttagtgcat aaacagagct tatttttaat
     6841 agtattaaat aagaaacaat ttgtacataa ttagggggag ttttaatgat aatgatggta
     6901 cttaataaga gtaagctcaa aaaaatcata atactaattt tagtagtgtt attagtatta
     6961 tcaggagggg taatggtatt aaagttattt aacaacaagc cagtatttaa tttatttaat
     7021 ggtgtagatt taccatatga aagaggtaca aaggataaaa gtggatatgt tgcattaacc
     7081 tgtaatatag accttggatg ggaaacagag tacgtagaat ccattttaga aacacttaaa
     7141 aaagaaaatg taaagattac atttaatgta actggaaaat gggcagaaaa gaataaggat
     7201 gaattattaa agataaaaaa acaaggacat gaaataggta atcatggata caaacattta
     7261 gattattcta cattatcata tgaagacaat tatgagcaaa tagagacctc taaaaagata
     7321 attgaagaaa ttataggtga aaaaactaaa ttttttcaag caccagcagg ttcttttggt
     7381 ccagaaacag taaaggctgc taaagcatta gggtatactt ctatcaaatg ggatgccgat
     7441 actatagatt ggaaatacaa agaccaacca gaggtaatca ttgatagaat gaagaaaaaa
     7501 gacattaaag atagtagtat aattttaatg catccaacca acgcaacgac aaaatgtata
     7561 gatgatatta tagctattgt aagagaaaaa ggactaaaac caggaaaact tagtgatgta
     7621 tttaagtaga caaagtataa atatttggtt aagattgtat aagttgtagt tgaattaatt
     7681 aaaagactga cttataatgt aatacatata aaaaataaat ataattaaaa catagtgttt
     7741 tcaataatcc aatatgaaac aaagaataaa ttgttaggag aatgtaatgt ataagacaaa
     7801 aatactggaa aatgggctta caataattgg tgaagaaata ccatatctaa agtcaataac
     7861 attaggtatt tggataaatg caggctctag gatagaagaa gcacaggtga gtggaacttc
     7921 tcattttata gagcatatga tgtttaaagg aaccaaaaac agaacatcaa aagagattgc
     7981 tagttcaata gataatttgg gtggtcaaat aaatgcattt acaagtaaag aatgtacatg
     8041 ttattatgta aaactaatag atgagcatat agatacagga attgatgtat tgagtgatat
     8101 gatactaaat tctaaatttg ataaaaatga tatagataag gaaagactta taatattgga
     8161 agaacttaag atgtatgaag actctccaga tgacttatct tatgatttat tagtggaaaa
     8221 tatatatgct aatgatggat taggaatgaa tataattggg acaaaagaat ctctatacaa
     8281 tataactaga gaatccatgt tagaatactt aaataagtat tatattccaa ataacgctgt
     8341 tatttctata gctggaaact ttaactttga tgatatggtg gagaaaatca aatcaaaatt
     8401 tggacactgg gaaaagaaaa atttaagtat agacataagt gaggctaagt ttaatccttg
     8461 ctttatatca aaaaataaag atactgaaca ggtgaactta gctatgtgcc taaaaggtat
     8521 tccttttgaa aatgatgaag aagtctattc aatggcagtg gtaaataata ttttcggtgg
     8581 aagtataagc tcaaggcttt ttcagaaaat aagggaagaa aaaggtttag tatattcaat
     8641 atactcatca cagacattat ataggaaatg tggtgaatta ggtatatttg caagcatgag
     8701 tacagaaaat ttacaagatg tatataattt aataaagaaa gaaattgaaa atataagaga
     8761 aaactatctt acagaaaaag aaatatctga aagcaaggaa cagttaaaag gaaattatat
     8821 attggattta gaaagtacta gcagtagaat gatgtctact ggtaaatcta tgttacttag
     8881 taaaaaagta aaaactacag atgaaatact tgaatgtata aataatgtta atattaattc
     8941 aataaagaag gttgttgata aggtattcaa tatcgaaaat attggtacat gtatagttgg
     9001 aagagatgta gaaaagattc tacacttgga ttaatattga aaaaagtaaa tctctggagg
     9061 tataaaatat gaatttatct gaaatagcag gtaaggaaat agttaacttg gtcacaggag
     9121 aaagattggg ggtcattgga gaatgtgacc ttatactaga tgaaactaca ggcggtatat
     9181 tagcgcttct aattccaaaa gaaaaaggat tttttgccag aagaaaagat aagtcatttt
     9241 tagaagtacc atggagaaat gtaaaaaaaa taggtaatga catgataatt atagaatatg
     9301 agggatatat ttaggaggaa aaaatgagta ttttagtgca aaagtttggt gggacatcag
     9361 tagaatcata tgaaaaaatg aatgaagtat gtaagatagt aaaagcatac aaaaaaaatg
     9421 atgaagaatt acaattagta ttagtagttt cagcaatggg aagaaaagga gctccatatg
     9481 ctacagatac acttataaat ttgtgtagtg cagtaaatga tgagccaagt aaaagagaac
     9541 ttgatttaat aatgtcttgt ggagaaataa tatcaggaac aattcttgct aatttattga
     9601 atgcacaggg gatagattct gttttcttta ctggtcagca agcaggtata attacaagtg
     9661 atgaatattc taatgcaaag ataaagtata taaatcctaa aaaggtaaaa agagctttag
     9721 atgatggtaa ggttgtagtt atagctgggt tccagggtgt aacagatgat ggagaaataa
     9781 ctactcttgg aagaggcgga agcgatacat cagcagttgc tattggtaaa gctttagaat
     9841 gtgaaacagt tgaaatttac actgatgtgg atggtataat gactgcagac cctagagtag
     9901 aaccaaacgc taaagtattg agttttatag actatgagga agtattccaa atggctgaca
     9961 aaggtgcaaa agtaatacat ccaagagcag tacagttggc taaaagtgga aatataactt
    10021 tagccataaa gaatacaatg aatccaactt ttgaaggtac aaaaataggt tctttatgta
    10081 gacacttaga agacaatata gaatatgaac aagaaagaga ttttaaggtt gctgttgcaa
    10141 ataaagatag cgtagcacaa gttaagatta agtcagcaga agaagtgttt acagaagtat
    10201 taaatgagat agaaaagaaa cttataacta tagatatgat aaatttcttt atatcagaaa
    10261 aagcatttgt tgtagaagat gctgatataa aatcattaaa ggaaatacta gataaatttg
    10321 aattagatta tgaagtaaat agagactgtg ctaaggtaac attgatatgt tcaagaattg
    10381 ctgatgaaat gtctggtatc atgtcaaagg tagttagagg attatcaaaa gcaggggtat
    10441 ctcttttaca aacatcagac tcaaatatga ctatatcttg tttagttagt gaagaagata
    10501 tgcatacagc agtacatgct atacaccaac aattttattt aaaataaact gtataataca
    10561 attatttttt aatcaaattt aagaatttca tatatatctt aatatatttg atttatttgg
    10621 taaaactaaa ttaaatataa ttttgatagg agttttttta tgaaagatta tagagaagat
    10681 aatgaaagat ttgataaaga taattgtagt gaagaatcta gttgcgaaga gagagaatgt
    10741 ataaagcaat ttggtaccaa tgaaatgcca cctcaacctc ctaaagatat tcaatgtata
    10801 actataattg gagaaattga gggacatttt ataggaaatc ctcaaaaaaa agctacaaag
    10861 tacgagcata taattccaat gctatactca attgaggaaa gtaatgatgt taaaggagtg
    10921 ttagttgttt taaacactgt tggaggagat attgaagcag gtttagctat tgcagaactt
    10981 ttaaacagta catctaaaaa agtagttact cttgttctag gaggaagtca tagtataggt
    11041 gttcctcttg ctacagcagg agattactca tttatagcac caacagcaac catgattata
    11101 catccagtgc gaactactgg tcttgtgatt ggtataaatg agacttttga atattttaaa
    11161 aagatgcaag atagaataat tcaatttata ataagaacat caaatatcaa aaaagatgta
    11221 cttgaaaaac ttatgcatga aaaagatgaa cttgtaagtg atgttggtag tgtattaata
    11281 ggaaaagaag ctgtggatta tggtcttata gatgaagttg gaggactaaa agaagcactt
    11341 aaaaaattaa gagagcttat aaaagaatct gaggaagaaa aaaataatga ttaaaatttg
    11401 tttaaataat aaaatttatg atataatgat gtaataaagt aggagtgtta gtgttaaggc
    11461 attagcactt tttgctgtga aggggtaaaa cttgctaaaa ataaggagtg atattaatgg
    11521 ccacgaaaaa gaagaaaaaa aagaaggtaa ataccttaag ttttaatgca gaatatcata
    11581 atttaataac aatatttata ggtgtatttt tattatatag tttaaattct aattctatgg
    11641 gatggatacc tgtgttaatg caaaatcttt ttaagggact atttggaggg ctttcaatag
    11701 caataccatt tatagtaatt ataacaggac ttttaggatt ttttgatgga aatgaatata
    11761 tatataggct aagaaagact aaactatact acataattat tttatttata tttgtatttt
    11821 atggattatt aaatgcagga actcttcctg tagatagccc actaaaaggt aacatgtttg
    11881 atgatgttat gaaattgggt gtaagtggac aaggtagtgg tttgatagca acaactattg
    11941 cgtattatat gagtaaaata tttggtatag caggtggatg gttaataagt atcttcgcac
    12001 ttatactttc tgtaatgttt atatttaata tttctataaa agatttggtg tcaaatgcaa
    12061 aatcaaaatc taaagcatct aaagacagca atttaacgtt taaagataaa attgcaaata
    12121 tgaaaaaatc tgcaatagat atgatgactg atgaagtaga tgatactact ataaataaaa
    12181 aaccaggttt ctttaaaggg ttaatgtcaa aaggtcttaa taatgatgat gaagaagatg
    12241 aatattttga agaagaaaat agtgatggtg tagatgataa aactataaaa atagttggat
    12301 ttaataaggc agaagatgag tatttagaaa tattagaagg aactcaaagt atgccagagt
    12361 tagatgtttt aaaagaatta caaaaagcta ccaatgaaaa tccagttgta gatactaaac
    12421 cagaaaaaaa agttgatata gctaaaccta atttaaatat agaaaaaact caacctatga
    12481 gtattgtagc agagccagtt aatgaggatt attcaaacta taaaaagcct agcattgagc
    12541 ttttaaataa agtaaataag aagtctgatg aaaatggtaa aaagaaagtg ttgaaaaatg
    12601 cttctctttt agaaaagaca ctatcagatt ttggagtgga agcaaaaatt aatcaagtta
    12661 cagttggacc tactataaca agatatgaaa tacaaccaag tccaggagtc aaggtaagta
    12721 aaattgtaaa tttaactgat gatatagctt taagtttagc agctaaaagt ataagaattg
    12781 aagccccaat tcctggtaaa agtgcaattg gaatagaagt tccaaatgaa gaagctcaaa
    12841 tggttggtgt gagagaagtt ctagaaagtg aagaatttaa taattttaac tctcctcttg
    12901 caatgggact tggaaaagac gtagcaggaa aaattataat tggagatata ggtaagatgc
    12961 cacacttact aatagctggt tctacaggtt caggaaagag tgtgtgtgta aatacattaa
    13021 taagtagtat tttatataag gcaaatcctg atgaagtaaa attactatta atagacccaa
    13081 aagtagttga acttgcaaat tataatggaa taccacatct attgatacca gtagttactg
    13141 accctaaaaa agctgcaaat gctttaaatt gggcagttac agagatgaat agaaggtaca
    13201 aattatttgc agatgctcaa gtaaaagatg taacaagtta taatgagaaa gcggaagaaa
    13261 aacttcctaa aatagtaata ataatagatg agttagcaga tttaatgatg gcaagtgcaa
    13321 atgatgtaga agattatatt tgtagattag ctcaaatggc aagagctgct ggaatgcact
    13381 tgattgtagc aactcaaaga ccatctgttg acgttataac aggggtaata aaggctaata
    13441 taccatctag aatagcattt gcagtatcat ctcaaactga ttcaagaaca atccttgata
    13501 tgggtggtgc agagaagctt ctaggtaaag gtgatatgtt attctatcca cttggtgcag
    13561 caaaaccagt tagacttcaa ggggcattca tatcagagag tgagtcggaa aaagtaatcg
    13621 attttgtaaa aagtcaggtt aaagatggaa taaaatatga agaagatata attgaaacta
    13681 tatctaaggt taatacttca aagggtagtg atgaggatga gtttttgagt gaagcgatag
    13741 aatttgtcgt ggaaagtggt caagcatctg cctctatgct acagagaaga tttaaaatag
    13801 gattcaatag agcagctaga cttatagatt ctatggaaga aagaggaata attggagcaa
    13861 gtgagggaag taaaccgaga aaggtattaa taagcaaaca ggatttgcag aatttagaag
    13921 gtgaatagaa ttgtcagtta taaaaataga aaaagcactt aagctagata aagtaatatt
    13981 tgtagatgtg agaactgaag gagaatatga agaagaccat atattgaatg caatcaatat
    14041 gcctcttttt aaaaataatg aacataatga agttggaaca atttataaga tgcagggaaa
    14101 acatgaagca atacaaaaag gatttgacta tgtatcgtat aaattaaagg atatatattt
    14161 acaagcagca gaacttgcat taaattatga taatattgtc atatattgtg caagaggcgg
    14221 aatgagaagt ggcagtatag taaatttatt atcatcatta ggtgtaaatg tatatcaact
    14281 tgaaggtgga tataaggctt atagaaattt tgttttagag tatttaaggc atgtaatgga
    14341 tacaaaaaac tttgtatctg ttcatggatt gactggtaca ggaaagactg atttgttaca
    14401 tttattagat gaaaaaggaa tagacgtgct ggatttagaa ggcatagcta aaaacagtgg
    14461 ttctaccttt ggttttataa cttttgataa aaagccacca tctcaaaaga actttgaaac
    14521 aaaactattt gaaagtatat ttttttcgaa agagaattat atatttgtcg agagtgaaag
    14581 caaacgagtt ggaagtgtta cagttccaaa tgaaattttc gaggcaatga cacgagaagg
    14641 acatcatata ttattagaat gtgatattga aaatagagtt gatagacttt gtagagatta
    14701 tatttatgat aaagatgaag gtaatatatt gattttaaaa gaatgtataa acaagtttag
    14761 aaaaagattg ggtaataaag tagtagatga ttatattgat ttattagaat caggtgaata
    14821 taaagaactt gtaaaaagat atcttttaga atactacgac ccactttata tgcattcagt
    14881 agagaaatat aagtatagta agattataaa ttttaatgat acacaaaaag ctttagaaga
    14941 tgttatagac ttatatgaga gtatattgga aggagaaagt gaatgttaaa aatagcattg
    15001 gaatcattag gatgctcaaa aaatttagta gatgcagaaa taatgatggg aattttaaat
    15061 aacaaaggct ataaattaat tggagatttt gaagaagctg atgtaattat agtaaataca
    15121 tgtgggttta tagagtcagc taaacaagaa tctatagata cgataataaa ctttgcagag
    15181 ttaaaaaaaa ctggcaattt aaagttgttg atagtaacag ggtgtctagc ccaaagatat
    15241 tctgaagaat taaaaactga aattcctgaa atagatgcta tagtaggaac aggaagctat
    15301 caaaatatag ataaaatact taaagaatta agtgaaatgc atcaaattgt aagccttaat
    15361 gatatagagt ttgtatttaa tgaagattta ccaagatata tatctacacc tagctatatg
    15421 gcctatttaa aaattggaga aggatgctcc aataattgta cttattgtat aataccaaaa
    15481 ctaagaggta aatatagaag tagaaaattt gaagatataa ttaaagaagc taagaaatta
    15541 gctgaatctg gagtaaaaga acttgtggtt atagcacagg atacgacaaa gtacggtttt
    15601 gatttatatg gaaaagaaag attatcagaa cttttagaag aacttgcaaa aatagatgga
    15661 tttaagtgga taagggttat gtattcatat ccagaatcta taacagaaga attgatacaa
    15721 gttataaaga aatatgataa tatatgtagt tactttgata tgccaataca gcatgcaagt
    15781 aacaatattt taaaattaat gaatagaaaa actactaaag aagatatatt aaataaaatc
    15841 aatctgataa gaagtaatat accagatgct atacttagaa ctacaataat agtagggttt
    15901 cctggtgaaa cagaagatga cttcaaacaa cttgtggact ttgttgaaga ggttaaattt
    15961 gatagacttg gtgcatttgc atattcaaga gaagaagata caccagcaga tagattgcca
    16021 aaccatatag atgaagaagt aaaaatacaa agaagagata ctttaatgat gattcaacaa
    16081 aaaatatctg aagaacttaa tgacaaaaag ataggaaaaa cttatgaggt gttaatagaa
    16141 gaacagatag aagataatgt atatacagga agaacacaag gtgatgcaga agaaattgac
    16201 agtatagtgt atgtaaaaag tgtagacaat cttgaagtag gagaatttgt aagtgttcaa
    16261 ataaatgatg caatggaata cgatttaatg ggagatgttt tatatgaact tgcctaacaa
    16321 gttaactttg tttagaatat ttttaatacc agtatttgtt ttaattatgt tgctcaatgt
    16381 accaaataaa tttttaatag cttgtattat ttttataata gcttcaatta ctgatgcttt
    16441 agatggaaaa atagctagaa aatacaatct agtcactgac tttggaaaat ttatggaccc
    16501 attagcagat aagttattgg ttatttcagc acttacatgt atgatagaag accatcttgt
    16561 aagtagttgg atggtaatta taatagtggc tagagagctt acagttagca tattaagagc
    16621 tatagctgct gctgatggaa aagtaattgc tgctggaaac agtggaaaac ttaaaacgat
    16681 aactcaaatg gtatctatag tgtttttatt gttaggtgct cagtttgaaa atgtattgat
    16741 attaaatatt ggtgaaattc tcattcttat agctacacta cttacattat attctggatg
    16801 ggaatatcta tataaaaata aagagctttt tatgtcttct aagtaaaaaa ttatgcttta
    16861 ataaataaaa actaatgatt aataaataaa aaataacaac ttaaaatttt aaaaatgtaa
    16921 aaaagggtat actattatag tatacctctt ttcattttaa gaatattttc ttttgtgtat
    16981 ataattatta ataatggttt ataaataaaa tgtaatgaat aaaaatataa tcattagtag
    17041 cgtttttaaa ttgactaggt ttgatttatg ttatataatt aatatataac aagagaacaa
    17101 atgtttgtag ataaatgaag ggatggtata gagatgagtg tagatcaaga aaaattaaaa
    17161 gcgttgaatg aagctttagg taaaattgaa aaagattttg gtaaaggttc agtaatgaaa
    17221 ttgggagaag caacatctat gtctatagat gttatatcaa caggagcgat tggtttagac
    17281 atagctattg gtataggagg tctacctaga gggagaatag ttgaagtata tggtccagaa
    17341 tcttctggta agactactgt tgcgcttagt tgtgtagcat cagctcaaaa agatggagga
    17401 atagctgcat ttatagatgc agaacatgca cttgacccag tatatgcaaa agctttgggt
    17461 gtggatgttg ataacctaat aatatctcaa ccagatacag gtgaacaggc tttagagata
    17521 gcagaggcat tgataagaag tggagcgata gatataatag taatagactc agtagcagca
    17581 ttagttccaa aggctgaaat agatggagat atgggtgatt ctcatgtagg tttacaagct
    17641 agacttatgt cacaagcact tagaaagtta actggttcaa ttaaaaaatc aaattgtgtt
    17701 gctatattta taaaccagtt aagagagaaa gtaggaataa tgtttggaaa cccagaaact
    17761 actactggag gacgtgcact aaaattctat tcatcagtta gattggatgt tagaaaaata
    17821 gatacaataa aacaaggtga taaagttata ggtagtagaa ctagagtcaa agttgttaaa
    17881 aacaaagtag caccaccatt taagcaagct gaatttgata taatgtatgg agaagggatt
    17941 tcaaaaattg gagacctttt agatatagct gctgatgtag atatagttaa aaaatcaggt
    18001 tcatggtata gttacaatga tactaaactt ggacaaggaa gagaaaatgt taaaaaattc
    18061 ttggaagata atttagattt aactactgaa atagatgaga aagttagagc attttacaat
    18121 ttaaatgaag aacatgaaga atcaggtact tcagtatcaa aagaaattgt agaagaataa
    18181 gtaaaaatac aaaaaaataa ctagtaatta aagacatgcc acgctcccta ttagcttgac
    18241 acaatgttga aaaaattata aaattaagtg aggataaaat atctaaattt aattttaatt
    18301 attaggaata ttttttaatc atagtgatga cattttggga ttattaacga aggaggtgga
    18361 tgtcatagat agtgtagtaa tgattgtaat aggtgcagct gttggtatta tagctggata
    18421 ttttgttaga aaaaatatat ctgaagcaaa aattggacaa gctgaaaact tggccaaaga
    18481 aataatagat aaagcacacc acgactcaga aacagtacaa aaagaaaaat tactagaagc
    18541 taaagaagag atccataagt ggagaacaga agctgaaaga gaaaacagag aaagaagaac
    18601 agaagtacaa aagtatgaaa aaagagtagt acaaaaagaa gaagtcttag atagaaaatt
    18661 acaaaattta gaatctaaag aagtaaacct aagtgaaaaa ttaaaggtag tggaaaagaa
    18721 agaagaagaa gttgaagtaa taaaaactca acagttagaa aaattagaaa gtatatcagg
    18781 aattacttct gataaggcca aagaaatcat cttaaccaat gcggagagag atgtaagacg
    18841 tgaaatgtct ataatgataa aagagataga aagtcaagct aaagaagaag ctgataagaa
    18901 atcaagagaa attataggat atgcaattca aaaatgtgca gcagatcacg tagccgaaac
    18961 tactgtaaca gtagtaagct tgcctaatga tgaaatgaaa ggtagaataa taggtaggga
    19021 aggtagaaat ataaggactt tagaaacctt aacaggtata gaccttataa tagatgacac
    19081 tccagaagcc gtaattttat caggatttga tccaataaga agagaaattg caagaattgc
    19141 tctagaaaaa ttaatagcag atggaagaat acatccagct agaattgaag aaatggttga
    19201 aaaggcgaga aaagaagttg acaatattat aaaagaatat ggtgaacaag ctgcatttga
    19261 aactagtgta catgggattc atccggaatt aataagatta ttaggtagac ttaactatag
    19321 aactagttat ggacaaaatg tacttaaaca ttctatagaa gttgctcata tagctggtat
    19381 aatggctgct gaaataggag cagatattag attggctaag agggcaggtt tattacatga
    19441 tataggtaag gctgtagacc atgaaatgga aggtacacat gttgaaatag gtatggattt
    19501 acttagacgt tataaagagt ctaaagaagt tatacatgcc atgagtactc atcatggaga
    19561 ttacgagcct caaacagtag aggctgtatt agttacagca gcagatgcaa tatcagctgc
    19621 tagacctggt gcgagaagag aaactttaga agcatatata aagagacttg aaaaattaga
    19681 agaaatagct aattcatatg aaggtgtaga aaaatcattt gctatacaag ctggtagaga
    19741 aataagaata atggtaaaac cagaagctat aaatgatgaa gaaatacatc tattggctag
    19801 agatatgact aagaaaatag aggatgaact tgaatatcct ggacaaataa aagtaagcat
    19861 aataagagaa acaagagcta tagagtatgc gaagtaaaag aaataatatc taaataataa
    19921 gaaaatattt ttagaaattt aaatttgcca taagatatga taatatatct tgtggctttt
    19981 aaattgttta agtttaattt tatataatgc aaaaataatc taagtgctaa aagtgctact
    20041 tacaaaagat taataaaata aagctttata tatgtattat ttatataatt atccaagttt
    20101 gtttgatata atgaagttgt aaaatcaata ctttactaca aaataaatag taaatactac
    20161 agtaaaatct aaaaatagga tataatagta ttaatataag aagattataa ataaaacctg
    20221 ctaatgaaaa tagattattt ttagcaggtt tttaaatttt ttataataaa tcttattttt
    20281 agtcaacaag atttattttt tttaaaactt tact
//
LOCUS       DBGJFF010000066        20205 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.067, whole genome shotgun sequence.
ACCESSION   DBGJFF010000066 DBGJFF010000000
VERSION     DBGJFF010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20205
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.067"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(224..1828)
                     /locus_tag="ACOIAF_002368"
     CDS             complement(224..1828)
                     /locus_tag="ACOIAF_002368"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HGZ2669376.1"
                     /translation="MKAKIYYNGNIITMEDSICGDAILIKDKIIKKIGTKEEVFALKN
                     KDTEIIDLQGKTLMPSFIDSHSHLIAFATTLKLVPLEDATSFKDIVKKIQDFKESNNI
                     KKGDWIIGFSYDNNFLEENKHPDKSVLDSASSENPILISHASGHMGVANTLGLKQLGV
                     TNETRDPEGGHIGRVEGSEEPNGYLEENAFFNVASKIKQPSSNEIFNSIEKAQNIYLS
                     YGITTAQEGLMEENQFNILKAMANQNKLKMDVVGYVNLKKSKSVADNNREFIKKYINR
                     FKIGGYKIFLDGSPQGKTAWLSRPYENSDDGYCGYPIYKDEEVEKFIDISLKEKMQLL
                     THCNGDAAADQLIDAFEKVLNLKEQSSENNIRPVMIHAQTVRADQIDDMKVINMIPSY
                     FVAHTYYWGDIHIKNLGEDRAFKISPLKTTIEKGLIYTLHQDTPVIAPNMLETVWCAV
                     NRITKKGIQIGENEKISPLDALKGVTINAAYQYFEEDKKGSIKEGKLANLIILDKNPL
                     TIDTMKIKDIKVLQTIREGEVLYSLK"
     gene            2214..3140
                     /locus_tag="ACOIAF_002369"
     CDS             2214..3140
                     /locus_tag="ACOIAF_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HGZ2669377.1"
                     /translation="MSKENKNVRIAVVQASPVIMDLEKTVEKVLGLIKEAGRKGANIV
                     VFPEAFIPAYPRGLSFGFVVGSRTMEGREDWKRYYDNSVPVPSATTDLLGKAAQEAGV
                     YLSMGITERDGNDINCTLYCTNLFFSPEGKLIGKHRKLKPTGTERCIWGEGDGSTLTV
                     VDTPYGKMGSLICWENYMPLARTALYAKGVKLYIAPTADSREEWQATMKHIALEGRCF
                     VIGCNQYVEKNMYPTDLNYYKELDAEPEIMCPGGSCIVDPFGKYVAGPIFNKEEMLIA
                     DLDLEKIVLSRLDFDSEGHYSRPDVFELIVHE"
     gene            complement(3260..4258)
                     /locus_tag="ACOIAF_002370"
     CDS             complement(3260..4258)
                     /locus_tag="ACOIAF_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdH/YhfP family quinone oxidoreductase"
                     /protein_id="HGZ2669378.1"
                     /translation="MNKKFKSLYITEEEGNFRRNIVEREVDTLPEGEVLIKVKYSSLN
                     YKDALSANGNKGVTRKYPHTPGIDASGIVESSSCDDFKIGEEVLVTGYDLGMNTSGGF
                     SEYIRVPSSWVVKLPKNLSLKEAMDYGTAGFTAALSVYKFIDSVDKNMGDVLVTGGTG
                     GVAVIATKILIKLGYSVVVSTGKLEEQKEVLLNLGVKDVIHRSEVDDNSGRPLLRPRW
                     AGVIDTVGGNTLATAIKTTNYCGAVTTCGNAGGVDFTSSVYPFILKGITLYGIDSVDC
                     PMNLRLKIWDLLSNEWKLDDLDDMVNVVSLEELNDSIDSIMAGKHVGRTVVDLEKI"
     gene            4362..4799
                     /locus_tag="ACOIAF_002371"
     CDS             4362..4799
                     /locus_tag="ACOIAF_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HGZ2669379.1"
                     /translation="MEDKYIVHFISKTKASMIKFIENKLSKNGLGELIPTHGNILTAL
                     YENNGILTMKEIAAKIGKDKSTVTVLVNKLINLGYLERQKCTNDKRITYIKLTKKALL
                     IEDTFNSISTQVKETAYHNITENEKQEFLRILKKINDNFKNAD"
     gene            4885..5472
                     /gene="rbr"
                     /locus_tag="ACOIAF_002372"
     CDS             4885..5472
                     /gene="rbr"
                     /locus_tag="ACOIAF_002372"
                     /EC_number="1.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016692 - NADH peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin"
                     /protein_id="HGZ2669380.1"
                     /translation="MNLKDSKTKENLLRAFAGESQARNRYNISASVAKKEGLYIIESL
                     FNYTADQEKSHAKQFFSKLKEFAGENISISGAYPVDLYDKTAQHLKAAQHNELQEWDD
                     VYKNFGKTAREEGFESIAKLFENIAEIEKVHGDRFGRYATDLEEGTLFRRNEDIQWIC
                     THCGHIHIGKEAPKACPVCLHPQGYFLDFKNSLFE"
     gene            complement(5772..6392)
                     /locus_tag="ACOIAF_002373"
     CDS             complement(5772..6392)
                     /locus_tag="ACOIAF_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbD family protein"
                     /protein_id="HGZ2669381.1"
                     /translation="MEELISKKDLLSKTNISYGQLYRWKRKNIIPEEWFIKKSAFTGQ
                     ETFFPRDKILERIELILSMKEETSLDDIANMFMKKDINKEFDIDFILSKEVICNYTKE
                     IFQNLYKKEQSIGKKELLILSIIEKFLVKSIITFEELKLIVNIIEENFTSIYNESGKI
                     YLFRKFGVPFVVGCLDYKKVKFEKDVVKIIEVDLTKEINDISIKLS"
     gene            complement(6400..7167)
                     /locus_tag="ACOIAF_002374"
     CDS             complement(6400..7167)
                     /locus_tag="ACOIAF_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HGZ2669382.1"
                     /translation="MEKGSIKILGEGFISSGEYENIKIVGSSKSKGNVKSNEVKVLGD
                     AKFEGDINIKNFVVNGKTIIRGNLKAERVRVNGQLNIEGDAEIDDLVITAQQKVDGKL
                     KCNNVTIRGQLSVNENMYAQDIKIYGEMRNKSNIECEDIKVYGAIKCDGLLNGENIYV
                     YSGGGSYCKEIGATNIKIGRSKDAFSLIKIINIFSGKFKCNLIEGDTIELEDASIKDI
                     RGKSISLVDNCDVGNIEYSDNLSVSDNSTVKNSIKVG"
     gene            complement(7208..8029)
                     /locus_tag="ACOIAF_002375"
     CDS             complement(7208..8029)
                     /locus_tag="ACOIAF_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669383.1"
                     /translation="MNEELSSLRFDGVGKCKGGIFDNVNISGTGKIEGSVKCSKFDSS
                     GSAKVFGTTECNEFFTSGLSKVEGNILASKVEISGLLRCTGNINSVEIDTSGIINVEG
                     SIKSDIIHGEGYLKANENIDCENINLSGILECNGFLNCEEVNISLNGTSNFNEVGASS
                     INIKKGHGDYCKKFRMLEKKHINFEKVSSNLISLGLLSIRKYTESKLFANIIEADEIS
                     LENSEVKVVRGKNIKIGKGCYVESLEYSESIEIDEDSIVGEIKNISEKIKLKKDK"
     gene            complement(8388..10298)
                     /gene="phnW"
                     /locus_tag="ACOIAF_002376"
     CDS             complement(8388..10298)
                     /gene="phnW"
                     /locus_tag="ACOIAF_002376"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HGZ2669384.1"
                     /translation="MKKIYGEKIKAVVFDWAGTTVDYGCFAPLNVFIEIFKRRGIDVT
                     MEEARKPMGKLKIDHIREMCEMDRIKNIWSDKFGKVPTEDDVNELYAEFEPMLFETLE
                     EYTTPIPHVVETIEKLRKNGLKIGSTTGYTREMMNIVEPNAAKKGYSPDFLVTPSEVS
                     QGRPYPWMCYKNAEALGVSPMSSMVKVGDTISDVKEGVNAGMWSVAVIKGSSELGLTQ
                     EEVENMDKEELKAKMSIVSKKFKEAGAHFVIETMAELEDILIKIENETIKSDFVPEND
                     YILLTPGPLSTTKSVRASMLKDWCTWDIEYNNLVQDVRRRLVSLATQNTDKYTSVLMQ
                     GSGTFSVEAIIGSTISKDGKLLVIANGAYGKRMKDICNYLNIEFVDCTFKDIEAVDLN
                     VVENLLKENKDITHISMVHCETTTGRLNPIQEVGKLAKEYNKIYIVDAMSSFGGIEID
                     VEDFNIDFLVSSSNKCIQGVPGFGFIIANKEELSKCKGIAKSLSLDVYAQWETMEKNN
                     GKWRFTSPTHVVRAFYQALLELEEEGSVEKRYARYKENQFTIASRLKSLGFDTLVNDN
                     AQSPVITTFLYPKNAKFEFMEFYTYLKDNGFVIYPGKLTDIDTFRIGSIGEVYPTDME
                     RLADVIEKFINR"
     gene            complement(10577..11362)
                     /locus_tag="ACOIAF_002377"
     CDS             complement(10577..11362)
                     /locus_tag="ACOIAF_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HGZ2669385.1"
                     /translation="MSLNSIDRKKEILKLLDSKGKVNTKELVKRLEVSSETIRRYLEE
                     LENENKLKKVYGGAIKINYGVIEPEHIKRNNTNLDKKQSIAKYAAKFVNDNESIIIDE
                     GTTNLQIVDYIISVNNLKIITNSYPVLSKLISYENQGLFDGEVIFIGGKVNLKHQRTA
                     CPMSIDTMKNLYVDKAFISSEGILDSFGISSIDQNKALLSKEYIKNSKESFVLCDSSK
                     IGIASMYKIADLEELDFVICDVEPPNEWKDKLNEINITWIEAK"
     gene            complement(11579..11809)
                     /gene="dltC"
                     /locus_tag="ACOIAF_002378"
     CDS             complement(11579..11809)
                     /gene="dltC"
                     /locus_tag="ACOIAF_002378"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HGZ2669386.1"
                     /translation="MQETVIEIFEDVLGTDEIREDLDLNLFETELLDSLAIIEVLLEI
                     ENRLGIELQPTDLERKDMSTVNNLVKFLETRK"
     gene            complement(11828..12976)
                     /gene="dltB"
                     /locus_tag="ACOIAF_002379"
     CDS             complement(11828..12976)
                     /gene="dltB"
                     /locus_tag="ACOIAF_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HGZ2669387.1"
                     /translation="MIFSQYGDYFYLYILLLTSIPAVILGLMGKNIKYYGMLASLFMI
                     FLIVGIDVQLKYLVIFIILEVIIVKGYEYVRRKTKNKYIYWGFLFASMLPIIINKISP
                     VTSFGIIGFIGISYLNFRTIQMVIEIYDGAIKEVKISKMLYFMLFFPTLSSGPIDRSR
                     RFEQDLEKQISRKEYIEEYLLPGIKNIVMGVGYKFVIAFLINTYWVSRIPKDMSFINI
                     LSYMYAYSLYLFFDFAGYSLFAIGTGYIFGIQVPINFDKPFISKDMKEFWTRWHISLS
                     RWFGDYIFSRFVMSSMRKKRFKKRTTAAHVAQMITMITMGFWHGLTWYYVAYGVYQGL
                     ALVLTDIYQRKSKFYKKHKKDKWFERVQIFITFHIVCFGLLIFSGYLA"
     gene            complement(12976..14490)
                     /locus_tag="ACOIAF_002380"
     CDS             complement(12976..14490)
                     /locus_tag="ACOIAF_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase"
                     /protein_id="HGZ2669388.1"
                     /translation="MKIIEGIKKYSNTDRTALMCNGDKLSYKDLNEYSDAISVFLKDV
                     YKEEDTPIVIYGNKENMIMACMIGALKSGRAYVPLDISFPIDRVFEVTKEIKPKVLFN
                     FSDERNFGDINVIDMDKLNYIINEYQGKSLDKENWVKDDENAYILFTSGSTGKPKGVQ
                     ISSNNLDSFSDWISPYLNIDGSEKVIMNQPAYSFDLSVTTIYPGLIHGATLFSISKDV
                     LADYKELFRQFSISDIAVWVSTPSFAGVCITEKEFNSKMLPNLESMIFIGEALSKNLT
                     KELMSRFPNTRIINGYGPTEATVGVSVNDMTQKAIDDEKSLPVGYPMSNCKIKILDED
                     GNELKENEKGEIIIIGPSVSKGYFNNKEKTDEVFFYDEIDGVKWRAYKTGDMGYLLDG
                     NIYYCGRKDFQIKLNGFRIEIEDIENNLRKVHNVKNAVVLPVYKDEKIAYLKGIVELN
                     EKNDLSNIKNGMIIKKELGKYIPSYMIPRNISIISEFPTNINGKIDRKKLMEEI"
     gene            complement(14611..15801)
                     /gene="dltD"
                     /locus_tag="ACOIAF_002381"
     CDS             complement(14611..15801)
                     /gene="dltD"
                     /locus_tag="ACOIAF_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HGZ2669389.1"
                     /translation="MRKLIYFITPFIIGVVFLFGLDKFLDSKTDELLREKNLLPIMDD
                     TLSDIKDKGVTANNHFLREKDIMILGSSELSNSTKQHPKYYFNTNRSKNKVFAIGRAY
                     TQTLQDAAILGSMNPNIDNKKVVLLISMQWFMEKDGVTSHHYQSRFSPIQFYRFLDNP
                     KISKQNKIEYAKKSSKLLWGSDEYKAEALYAKLYEPKTFPEKAEKVLLEPYFQGRKYC
                     IALKEKGILYKRLIKLDKKRATKRKRPINWSYERKKAIEDAKKRVGKNPLNIDKYYYK
                     EHFKDGIEQYKGRDKDVNLLTSKEFENYKLMLNVCTDLGIKPVVVLIPSMDKFYNLTG
                     ISEKERNQYYDKAQKIAESKGFEVLNLKDKGSDKYYLRDVMHLGTKGWVDVCERLFKI
                     FKEQ"
     gene            complement(16089..17108)
                     /locus_tag="ACOIAF_002382"
     CDS             complement(16089..17108)
                     /locus_tag="ACOIAF_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HGZ2669390.1"
                     /translation="MMGKIRFGIVGTSNIANIFLRAASRVKDFELVAVYSRNLEKARE
                     FGTLYGASIFFDDLEQMAKSKDIDAVYIASPNALHSKQTITCLKHKKHVLCEKSLASN
                     LKEVKSMIKTAKDNNVLLMEAMRITCVPNFKAVKENLYKIGKIRRFFGSYCQYSSRYD
                     KYKNGIIENAFKKELSNGALMDIGVYCIHPMVNLFGAPKTVKAVSHILQTGVDGEGSA
                     VFQYDDMDAIIQYSKVADSYIPSEIQGEEGSIIIEKLNLFEKAIIKYRDGREEDISIP
                     KEKAEEKPREIEGMYYELVEFISLINNNKIESNINSHENSIIVMEIMDEIRRQSSIVY
                     PADSI"
     gene            complement(17284..17823)
                     /locus_tag="ACOIAF_002383"
     CDS             complement(17284..17823)
                     /locus_tag="ACOIAF_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HGZ2669391.1"
                     /translation="MDNVGSLLYFLFNFFLYGFIGWIIENLYSYYKQGHFQEDGFLNG
                     PFKPMYAIAMSIIIEVSRFIDPNNIFALLLLCLVVPTVVEYITGILMKVYFNKVYWDY
                     SDYKYNSRGIVCLKFSVYWTILTFIGVRYFQVYIVDSLYSLIIPYWPIVVTILLVTLF
                     VDEILTIKFLRGNKDIVER"
     gene            complement(17924..18604)
                     /locus_tag="ACOIAF_002384"
     CDS             complement(17924..18604)
                     /locus_tag="ACOIAF_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HGZ2669392.1"
                     /translation="MQKVEGIIFDMDGVLFDSERISLEFWMETFEKYGYTMTKEIYTS
                     VMGRNRKGIIEGLTDIYDSSVPIIDLYDEKTKNMIEFMERKGAPIKLGVNELISFLKE
                     NGYKMAVATSTKRERAVKRLAKANLKDYFDAIVCGDDVVNSKPNPEIFLKAAKKINVN
                     PKNCIVIEDSPMGVEAAYNGGIRCINVPDLKEPDEQIKSQSHKILENLLEVREYLKSL
                     NSKECHNL"
     gene            complement(18633..19922)
                     /locus_tag="ACOIAF_002385"
     CDS             complement(18633..19922)
                     /locus_tag="ACOIAF_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HGZ2669393.1"
                     /translation="MRELLKVTLIVMLVLINAFFVACEFAMVKLRSSRIDTMITEGNN
                     NAKRCKIIKDNLNSYLSACQLGITLCSLALGWMGESTVKELILPIVSVFNLSESIIYT
                     ISISISFLIITMMEVVIGELVPKALALYNTEKIMLSTSFLLVGFYKLTYPIIYFFNLS
                     TDLFLKPFGYSQADEVDEPHTGDEIRLLVEESYKSGLIDESEQRLVDNIFEFEEKKIR
                     EIMVPRTDMVCIYESDSEEKILAILKEEGVTRYPVCRKNKDDILGFVHIRDLYNQKIN
                     ENKIELEEILRDIIYISENLTIDKALERIRKEKLQLAIVVDEYGGTSGVVTIEDILEE
                     IVGEIQDEFDKEETHIKKIGAGSYLVDGTESINYINKYFGLDIEHDGFDSIGGWISYM
                     LGSNIKVNQSVTFENYNFTILELDKLRVVKLIIKSVI"
BASE COUNT         6648 a         3494 c         2064 g         7999 t
ORIGIN      
        1 aattaataac tacttaattg tttaatatag tacatagata actttaataa atcgatagtt
       61 atttaaaaag gtagaatcaa taaaattata cttataaatc ttcaatacaa taattaaaat
      121 ttaataaaaa tataattgtt gatttaactg taatcttatt taattctacc taacttatta
      181 tataaaacta ttttttatag attataaaat atctaacttt catctacttc aaagaataca
      241 atacttcccc ttctcttata gtctgcaata ctttaatatc ttttattttc atagtgtcga
      301 ttgtaagtgg gtttttatct aatattatca aattagctaa tttaccttct ttaattgaac
      361 cttttttatc ttcttcaaaa tattgatatg ctgcatttat agtaacaccc tttaaagcat
      421 ctaatgggct tatcttttca ttttctccta tttgtatacc tttttttgta attctattga
      481 cagcacacca tactgtttcc agcatattag gtgctattac tggtgtatcc tgatgaagag
      541 tgtaaattaa tcccttttct atagttgttt taagtgggct aatcttaaac gctctatctt
      601 ctccaaggtt tttgatatga atatcacccc aataatatgt gtgagctaca aagtatgaag
      661 gtatcatatt tattactttc atatcatcaa tctggtctgc tcttactgtt tgagcatgta
      721 tcataactgg tcttatatta ttttcactac tttgctcttt taaatttaaa actttctcaa
      781 acgcatctat caactggtct gctgctgcat ctccattaca atgagttaag agttgcatct
      841 tttcttttag tgaaatatct ataaactttt ctacttcttc atctttgtat ataggatatc
      901 cacaataacc atcatctgaa ttttcatatg gtctactcaa ccatgctgtc ttaccttgtg
      961 gagaaccatc taagaaaatc ttataacctc ctattttaaa tctatttata tattttttta
     1021 taaactccct attattatct gctacagact tacttttttt caaatttaca tatcctacaa
     1081 catccatttt taatttattt tgatttgcca ttgcttttaa aatgttaaat tgattttctt
     1141 ccattaaacc ttcttgagct gttgtaatac catagcttag atatatgttc tgtgcttttt
     1201 caattgaatt aaaaatctca tttgatgatg gttgttttat tttagatgct acattaaaaa
     1261 atgcattttc ttctaaatat ccatttggtt cttcactgcc ttctactcta cctatatgtc
     1321 caccttctgg gtcacgtgtt tcattggtaa caccaagctg ttttaacccc aatgtatttg
     1381 caactcccat atgaccagaa gcatgtgaaa ttagtattgg attttctgaa gatgcactat
     1441 cgagtacact cttatctgga tgcttatttt cttctaaaaa attattatca tatgaaaatc
     1501 caattatcca gtctcctttt tttatattat tcgactcttt gaaatcctgt atttttttta
     1561 ctatgtcttt aaaactggtt gcatcttcta aaggaactag ctttagagta gtagcaaaag
     1621 ctatcaagtg actatgtgaa tctataaaag atggcatcaa tgtcttacct tgtaaatcta
     1681 taatttctgt atctttattt ttcaaagcaa atacttcttc ttttgttcct atctttttta
     1741 ttattttgtc ttttataaga atagcatctc cacatattga atcttccatt gtgatgatat
     1801 ttccattgta ataaattttt gctttcataa aattctcctt acgttgtcta ctttaataaa
     1861 tttttttcat tattttctgc ttaattatat tatcttcttt ataaactttt tctttagagt
     1921 ttaatcatat aaaagtttta tttttaatta ttgtttgcca agaagtaaat actattcatt
     1981 gtaaataaga caaattaata ggacttatac taattgaaaa aatattataa gttgttaaat
     2041 ttaaatatct aaactcaatt atatctgtac aattgtaatt ttttaaatca tgatgtatta
     2101 ttttaaatat acagttacat tattattcac aatataagta tcttagttct caaataacat
     2161 ctcatgtaat ctattcaatg caaaaaattt aaatattttt taggaggttt tttatgagca
     2221 aggaaaataa aaatgtaaga attgctgttg tccaggcgtc accagtcata atggatttag
     2281 aaaaaactgt tgaaaaagta ttaggattaa ttaaagaagc tggtagaaaa ggggcaaata
     2341 tcgtagtttt ccctgaagca ttcatacctg catatccaag aggtctttca tttggatttg
     2401 ttgttggaag tagaacaatg gaaggtagag aagattggaa aagatactat gataattctg
     2461 taccagttcc tagtgctaca acagatttat taggaaaagc agcacaagaa gcaggagtat
     2521 atctatcaat gggtataaca gaaagagatg gtaatgatat aaattgtacc ctttattgca
     2581 caaacttatt tttctctcca gaaggtaagc taattggtaa acacagaaaa cttaaaccta
     2641 caggaacaga aagatgtatt tggggagaag gagatggaag tactcttaca gtagttgata
     2701 ctccatatgg aaaaatgggt tcgttgattt gttgggaaaa ttatatgcca ttagctagaa
     2761 cagctcttta tgctaagggt gtaaaacttt acatagcccc tactgcagat tcaagagaag
     2821 agtggcaagc aactatgaaa catattgctt tagaaggaag atgttttgta attggatgca
     2881 atcaatatgt tgaaaaaaat atgtatccaa ctgaccttaa ctactataaa gagctagatg
     2941 cagaaccaga aattatgtgt ccaggaggaa gttgtatagt tgaccctttt ggaaaatatg
     3001 tagcaggtcc tattttcaat aaagaagaaa tgctaatagc tgatttagac ctagaaaaaa
     3061 tagtacttag tagactagat tttgattcag aaggtcacta ttcaagacca gatgtatttg
     3121 aattaattgt tcatgaataa tctttttaca caaacatcta cataaatctt tttacataaa
     3181 aaaatgagat actcataagt aaatttcaag tatctcattt ttgcttaaaa cctataatcc
     3241 taattataaa tttattcacc tatatttttt ctaaatcaac tactgttctt ccaacatgtt
     3301 ttcctgccat tattgaatct atactatcat ttaactcttc caatgatact acatttacca
     3361 tatcatctaa atcatctaat ttccattcat ttgataataa atcccatatt tttaatctta
     3421 agttcattgg gcaatccaca gaatctatac catataaagt aattcccttt aatataaatg
     3481 gatatactga acttgtaaaa tcaactcctc ctgcattacc acaagttgtt actgctccac
     3541 aataattagt tgtctttatt gctgttgcta gagtatttcc tcctactgta tctattactc
     3601 cagcccatct tggtctaagc agaggtcttc ctgagttatc atccacttct gacctatgta
     3661 taacatcttt aacacctaag tttagtaata cttctttttg ttcttctaat tttcctgttg
     3721 aaactacaac tgaataacct aatttaatca atattttagt tgcaattaca gcaactcctc
     3781 cagtaccacc agtgactaat acatctccca tattcttgtc tacagaatct ataaatttat
     3841 acacagataa agctgctgta aaccctgctg taccataatc cattgcttct tttagtgaaa
     3901 gatttttagg aagtttaact acccaactag aaggaactct tatgtattct gaaaaaccac
     3961 cagaagtatt cattcccaag tcataacctg ttactaaaac ttcttctcca attttaaaat
     4021 catcacaact actagattct actataccag atgcatcaat tcctggtgta tgaggatact
     4081 ttctcgtaac acccttatta ccatttgcag aaagtgcatc cttgtaattt agagaagaat
     4141 attttacttt tattagaact tcaccttctg gcaatgtatc tacttctctt tcaactatat
     4201 ttcttctaaa atttccttct tcttctgtaa tatatagtga tttaaatttt ttattcataa
     4261 atttcctccc aatacattta gttttatgtt aaactaatta tagtttataa atagttttat
     4321 gtcaaactaa tttaaataga ttaaagattg gagaataatt tatggaagat aaatatattg
     4381 tacatttcat aagtaaaaca aaggctagta tgattaaatt cattgaaaat aaactcagta
     4441 aaaatggttt aggtgagtta atacctactc atggaaatat tttaactgca ctttatgaaa
     4501 ataatggaat acttacaatg aaagaaattg cagctaaaat aggaaaagat aaatccactg
     4561 ttacagtgtt ggttaataaa cttataaacc taggatattt agaaagacaa aaatgtacaa
     4621 atgacaaacg tatcacgtat ataaagctta caaaaaaggc actgcttata gaagatactt
     4681 ttaatagcat ctcaacacaa gttaaagaga cggcatatca taatattaca gaaaatgaaa
     4741 aacaagaatt tcttagaatt ttaaaaaaaa ttaatgataa ttttaaaaat gctgactaaa
     4801 ttgagcataa ttatatctcc tattggaata ataaataaaa agaatcaaat attgtaaaaa
     4861 tttattgtga tagagaggta gaatatgaat ttaaaagata gtaaaactaa agaaaattta
     4921 ttaagagctt ttgcaggtga gagtcaagct agaaatagat acaatatttc tgcatctgta
     4981 gcaaaaaaag aaggattata tataatagag tctttattta attacactgc tgaccaagaa
     5041 aaatcacatg caaaacaatt cttttctaaa ttaaaagaat ttgctggtga aaatataagt
     5101 atatcaggag cataccctgt agatttatat gacaagacag cacaacacct taaagctgca
     5161 caacataatg agcttcaaga gtgggatgat gtatataaaa actttggaaa aacagcacgt
     5221 gaagaaggat ttgaatctat agctaagtta tttgaaaata tagctgaaat agaaaaagtt
     5281 catggtgata gatttggtag atatgctact gatttagaag aaggtacttt attcagaaga
     5341 aatgaagata tacaatggat atgtacacat tgtggccata tacatatagg taaagaagct
     5401 ccaaaagctt gtcctgtatg tcttcatcca caaggatatt tcttagattt taaaaactca
     5461 ttatttgaat aaattaaggc aataattcta tttaaacaat ttactatatt tatatcaagt
     5521 atttttataa atactgtatt atcttaataa caataaaatg aaccattgat attactataa
     5581 gtagaaaatg tagtttaaat acaatttata gtagttcatc atggttcatt ttattgttta
     5641 ctaatcccta taacctactt aaatctatct ttaactttta cacactcaca taaaatctca
     5701 tctttaaact aaatatttaa ataaagtgta ttttaagtag attattataa tacattatat
     5761 aagataatac actatgataa ctttatactt atatcattta tttcttttgt caagtcaacc
     5821 tcaataatct taactacatc tttttcaaac tttacttttt tataatccag acatcctact
     5881 acgaatggaa ctccaaactt tctaaacaaa taaatctttc cactttcatt gtatattgat
     5941 gtaaaatttt cttctattat atttactatc aattttaact cttcaaaagt tattatagat
     6001 ttaactaaaa acttctctat tatgctcaat ataagcagtt cttttttacc tatgctttgc
     6061 tcttttttat ataaattttg aaatatttct ttggtataat tacatataac ttctttactt
     6121 agaataaaat caatgtcaaa ttctttatta atatcctttt tcataaacat atttgcaata
     6181 tcatcaagag aagtctcttc tttcatagac aatattaact ctattctttc tagtatttta
     6241 tctcttggaa agaaagtctc ttgacctgta aaagctgatt tttttataaa ccattcctct
     6301 ggtattatgt tttttctttt ccatctatat aattgaccat aagatatatt agttttactc
     6361 aataaatctt tcttagatat taattcttcc ataacttttc tagcccacct ttatactgtt
     6421 ttttacagta gaattatctg aaactgataa attatcactg tattcaatat ttccaacgtc
     6481 acaattatct actagagata tgcttttacc tcgtatatct tttattgatg catcttctaa
     6541 ttcaatagta tctccctcta tcaaattaca tttaaacttt cctgaaaaaa tattaatgat
     6601 tttaattaat gaaaaagcat ctttgcttct tcctattttt atattagttg cccctatctc
     6661 tttacaataa gaacctcctc ctgaataaac atatatattt tctccattta aaagaccatc
     6721 acattttatt gctccatata cctttatatc ttcacattct atatttgatt tatttctcat
     6781 ttcgccatag atttttatat cttgtgcata catattttca tttacactta attgtcctct
     6841 tattgtaaca ttattacatt taagtttgcc atccactttc tgttgagcag ttataactaa
     6901 gtcatctatt tctgcatctc cctctatatt aagttgacca ttcactctta cacgctctgc
     6961 tttaagattg cctcttataa tagtttttcc gttaacaaca aaatttttta tattgatgtc
     7021 accttcaaat tttgcatctc caaggacttt tacttcgtta cttttaacat ttccttttga
     7081 tttagaactt cccacaattt ttatattttc atactcacca ctacttataa agccttctcc
     7141 aagtattttt atagaccctt tttccatatt attacctcat tttaaatttt atttttaaac
     7201 ctacttatta cttatccttc tttaatttta ttttttcact tatattcttt atttcaccaa
     7261 ctattgaatc ttcatcaatc tctatacttt cactgtactc caaactttct acataacatc
     7321 ccttgcctat tttaatattt tttcctctta caactttgac ttcactattt tctaagctta
     7381 tttcatctgc ttctataata tttgcaaaca atttactttc tgtatacttt ctaattgata
     7441 atagacctaa acttattaaa tttgaagaca ctttttcaaa gttgatatgc tttttttcta
     7501 gcatacgaaa ttttttgcaa taatctccat gccccttttt tatatttata ctacttgctc
     7561 caacttcatt aaaattagaa gttccattta aactaatatt aacttcttca caattcaaaa
     7621 atccattaca ctccaagatt ccacttaaat tgatattttc acaatctata ttctcatttg
     7681 cttttaaata tccttctcca tgaataatat ccgattttat acttccttct acattaataa
     7741 ttccactagt atcaatttca acagaattaa tatttcctgt acatctaaga agtcccgata
     7801 tctcaacttt tgatgccagt atgtttcctt ctactttact taaaccagat gtaaaaaatt
     7861 cattacattc tgttgtccca aaaacttttg ctgaaccaga agaatcaaat ttactacatt
     7921 ttacacttcc ttctattttg cctgttcctg atatattaac attatcaaaa attccacctt
     7981 tacattttcc aacaccatca aatcttagtg aacttaattc ttcattcata atgatatcct
     8041 ttctttaccg tattttatct ttaaacctaa tataacaaaa cattgttacg ttgtcaagta
     8101 tgataataat acttaataaa gcaactatca tttattttct ataaataggc ataaaaataa
     8161 tagggaaatt ctttatttaa actataaatc taatttctta gattgtggtt taaataaaat
     8221 atatccctat tactcaatct ttatatattc attgtataaa cttctaaaat ataagtcttt
     8281 aaattctact gtcaatactt tttatacact gtttgtgcta tcatctcaca cacctaaagg
     8341 agtgggcttt ccgcactatt ctgtaaccta caagacacta tcttcttcta tctattgata
     8401 aatttttcta ttacatcagc tagtctctcc atatcagttg gataaacttc acctatgcta
     8461 cctattctaa atgtatctat atcagtcaat ttacctggat agataacaaa tccattatct
     8521 tttaaataag tataaaattc cataaattca aactttgcat ttttaggata taagaaagtt
     8581 gttattacag gtgattgagc attatcattc accaaagtat caaatcctaa agattttaat
     8641 cttgaagcta ttgtaaactg attttcttta tatctagcat atcttttttc aacagaacct
     8701 tcttcttcca attctaataa agcttgataa aaagctctta caacatgtgt aggtgaagta
     8761 aatctccatt taccattatt tttctccata gtctcccatt gcgcatacac atcaagagat
     8821 aatgatttag ctataccttt acatttagat aattcctctt tattagctat tataaaacca
     8881 aatccaggta ctccttgtat acatttgtta gaactactaa ctaaaaaatc tatattaaaa
     8941 tcttcaacat caatctcaat tcccccaaaa ctactcatag catcaactat ataaattttg
     9001 ttatattctt ttgcaagttt ccctacctct tgtataggat ttaatctacc tgtagtagtt
     9061 tcacagtgaa ccattgatat atgtgttata tccttgtttt cttttaataa attttctact
     9121 acatttaaat ccactgcttc tatatcttta aaagtgcagt caacaaattc tatgttaaga
     9181 taattacata tatctttcat tctctttcca tatgctccat ttgcaattac aagaagttta
     9241 ccatcttttg aaatagtact tcctattata gcttctactg aaaaagtccc acttccttgc
     9301 attaatacac ttgtgtattt atctgtattt tgggttgcta gagatactaa tcttcttcta
     9361 acatcttgta ctaggttatt atattcaata tcccaagtac accaatcttt taacatagat
     9421 gctcttactg atttagtagt agatagtggt cctggtgtaa gtaatatgta atcattttct
     9481 ggtacaaaat ctgatttaat agtttcattc tctattttta ttagtatatc ttctaactca
     9541 gccatagttt ctattacaaa atgtgcacct gcctctttaa atttctttga aactatactc
     9601 atcttagctt ttaattcttc cttatccatg ttctctactt cttcttgtgt aagccctaac
     9661 tcacttgaac cttttattac agcaacactc cacattcctg catttacgcc ttcttttaca
     9721 tcacttattg tatctcctac ttttaccata gaactcatag gagacacccc taaagcttca
     9781 gcatttttat agcacatcca aggatacggt cttccttgag atacttctga tggtgttaca
     9841 agaaaatcgg gtgagtatcc tttctttgct gcatttggtt caactatgtt catcatctct
     9901 cttgtataac ccgttgtaga acctattttt aatccatttt ttctcaactt ttcaatagtt
     9961 tcaacaacat gaggaattgg agtcgtatac tcttctaatg tctcaaataa cattggctca
    10021 aattcagcat aaagttcatt tacatcatct tcagttggca ctttaccaaa cttatctgac
    10081 caaatattct ttattctatc catttcacac atttctctta tatggtctat ttttaatttt
    10141 cccataggtt ttcttgcttc ttccattgtt acatctatac ctcttctttt aaatatctct
    10201 ataaatacat taagaggtgc aaaacatcca taatctactg ttgttccagc ccaatcgaat
    10261 actactgctt ttattttttc tccatatatc tttttcatat ttatagcctc catagattct
    10321 ttttagaatt tttatgtttt taattgtttt tacttttaaa gtataacttt catatgttaa
    10381 ttatacatca tatttaggtt aaatatatat taaatttctg tttttagttg tatttagttg
    10441 tttttatttt gtatcacatt cccataaaat cctaagttac aatctaaaat ttactttcta
    10501 atcttaatct atgaactgac tatattttat gaatttagtt gtattgagca tagttgtatt
    10561 atataaaact aagggtttat tttgcttcta tccatgttat atttatctca tttaacttat
    10621 ccttccattc attcggaggt tctacatcac aaataacaaa atctaattcc tctaaatctg
    10681 ctatcttata catactagca attcctattt tagaactatc acataataca aaactttcct
    10741 ttgaattttt tatatattct tttgacaaca atgccttatt ttggtctata ctactaattc
    10801 caaaactatc aagtatacct tcactagata taaaagcttt atctacgtat aagtttttca
    10861 tagtatctat tgacattgga catgctgtcc tttggtgttt taaatttact tttccaccaa
    10921 taaatataac ttctccatca aacaaacctt gattctcata agaaatcaat tttgatagaa
    10981 ctggataaga gtttgtaata atttttaaat tgtttacaga tattatataa tctacaatct
    11041 gcaaattggt agtaccttca tctatgataa ttgactcatt gtcatttaca aatttagctg
    11101 catatttagc aatactttgc tttttatcta ggttagtatt gtttctcttt atatgttctg
    11161 gttcaatcac tccataattt atttttatag caccaccata tacctttttt aatttatttt
    11221 cattttctaa ttcttccaaa tatcttctta ttgtttctga tgatacttct aatcttttta
    11281 ctaattcttt agtgtttacc ttcccttttg aatctaataa tttaagtatt tctttttttc
    11341 tatctatcga atttaaagac attcattttc ctcctaaata taatatattt ttctcctata
    11401 atattacaat gatacctaag atttatgaaa tcatatttat gtattaaatt ttttacttaa
    11461 ttaaagttat tttcaaaaga ttttgtatta tcattaaaaa agtccatcaa atattagtat
    11521 cagatggact ttgtaaaatt ttattttata aattaatatt taaagtctaa ctactaattt
    11581 attttcttgt ctccaagaat tttactaaat tatttacagt agacatatct tttctctcta
    11641 aatcagttgg ttgaagctct atccctaatc tattttcaat ttcaagtaaa acttcaatta
    11701 tagctaatga gtctaaaagt tccgtctcaa ataaattaag gtctaaatct tctcttatct
    11761 catcagtccc taatacatct tcaaatattt ctataactgt ttcttgcata aattttcctc
    11821 cttaaaatta agctaaataa cctgagaata tcagcaatcc aaaacacaca atatggaatg
    11881 taatgaaaat ctgcactctc tcgaaccatt tatctttttt atgtttttta taaaatttag
    11941 attttcgctg atatatatca gttaacacaa gtgccaatcc ttggtataca ccataagcaa
    12001 cataatacca agttagacca tgccaaaatc ccattgttat catggttatc atctgagcca
    12061 catgagcagc agtagtcctt ttcttaaatc ttttctttct cattgatgac ataacaaatc
    12121 tagaaaaaat ataatctcca aaccatcttg aaagacttat atgccatctt gtccagaact
    12181 ctttcatatc tttactgata aatggcttgt caaagtttat aggtacttgt attccaaata
    12241 tgtaccctgt acctatagca aataaactat atcccgcaaa atcaaagaac aggtataaac
    12301 tatatgcgta catataactt aaaatattta taaaactcat atctttaggt attcttgata
    12361 cccaatatgt atttatcaaa aatgctataa caaacttata acctactccc atgacaatat
    12421 tttttattcc tggcagtaag tattcttcta tatactcttt cctagagatt tgtttttcca
    12481 agtcctgttc aaatcttctt gacctatcaa taggtccaga acttaaagtt ggaaaaaata
    12541 acataaaata aagcatcttt gaaattttaa cttcttttat agctccatca taaatttcaa
    12601 taaccatttg tatagttctg aaatttaagt atgatattcc aataaatcca attattccaa
    12661 aagaagtaac tggagatatt ttatttatta ttattgggag cattgatgca aacaagaatc
    12721 cccaatatat gtatttattt ttagtttttc ttcttacata ttcatatcct ttaactatta
    12781 ttacttcaag aataataaat attaccaagt actttagttg gacatcaatt cctactatta
    12841 agaatatcat aaacaaactt gccaacatac cataatactt tatattcttg cccattagac
    12901 ctaaaattac tgctggtatt gatgttaata ataatatata aagataaaaa taatctccat
    12961 attgtgaaaa aatcattaaa tttcctccat aagtttcttt ctgtctattt taccattgat
    13021 atttgttgga aactcagaga ttattgaaat atttcttgga atcatatatg aaggtatata
    13081 ctttccaagt tcctttttta ttatcatccc atttttaata ttacttaaat cattcttttc
    13141 atttaactca acaatacctt ttaagtaagc aattttttcg tccttatata ctggtaaaac
    13201 tactgcattt tttacattat gtacttttct caaattattt tctatatctt ctatttcaat
    13261 tctaaatcca tttaatttaa tctgaaaatc ttttcttcca caataatata tatttccatc
    13321 taaaagatat cccatatctc cagttttata agctctccac tttacaccat ctatttcatc
    13381 atagaaaaat acttcatcag tcttttcttt attattgaag taacctttag aaactgatgg
    13441 tccaattatt ataatttcac ccttttcatt ttctttaagc tcatttccat cttcatcaag
    13501 tatttttatt ttacaatttg acatagggta tccaactgga agactttttt catcatctat
    13561 agccttttga gtcatatcat ttacacttac accaacagta gcctcggttg gtccatatcc
    13621 attgataatt cttgtgtttg gaaatctact cataagttct ttagtaagat tttttgaaag
    13681 tgcttctcct atgaatatca ttgactcaag gttaggaagc attttactat taaactcttt
    13741 ttcagttata catactcctg caaaagaagg agttgacacc catacagcta tatctgagat
    13801 actaaattgt ctaaatagtt ctttatagtc tgctaataca tcttttgata ttgaaaataa
    13861 tgtagctcca tggataagac cagggtatat tgttgtaact gaaaggtcaa aagaatatgc
    13921 tggttggttc attattactt tttcacttcc atctatgttt agatatgggc ttatccaatc
    13981 tgaaaagcta tctaaattgt ttgaacttat ttgaacccct tttggctttc ctgtacttcc
    14041 tgaagtaaat agtatatatg cattttcatc atcttttacc cagttttctt tatctaatga
    14101 ttttccctga tattcattta ttatataatt taatttatcc atatcaatta cattgatatc
    14161 tccaaaattt ctttcatcac taaagttaaa taataccttt ggttttatct ctttagtgac
    14221 ttcaaaaact ctatcaattg gaaaactaat atcaagaggc acatacgctc tacctgattt
    14281 taaagctcct atcatacatg ccattatcat gttttcttta tttccataaa ttactatagg
    14341 agtatcttct tctttgtata catcctttag aaatactgat atagcatctg aatattcatt
    14401 taaatcttta taagaaagtt tatctccatt acacattagt gctgttctat ctgtatttga
    14461 atattttttt attccttcaa taattttcat ataagaatgc tcctttaaaa tccgttataa
    14521 ataaatggca attctttaaa tggagtaaat gttagaactc ctatcgccaa tattataacc
    14581 atagttaaac aaatactagt taacaaatct ttattgttct ttaaatattt taaataatct
    14641 ctcacagaca tctacccatc ctttcgtacc aagatgcatt acatccctta aataatactt
    14701 atctgaccct ttatctttaa ggtttagaac ttcaaagcct ttgctttctg ctattttttg
    14761 tgctttatcg tagtactgat ttctttcttt ttcagaaata ccagtcaaat tatagaattt
    14821 atccatactt gggattaata caacaactgg cttaatacct aagtcagtac atacatttag
    14881 catcagttta taattttcaa actccttaga tgttaataaa tttacatctt tgtctcttcc
    14941 tttgtattgc tctataccat ctttaaaatg ctctttataa tagtacttat caatatttag
    15001 tggattttta ccaactcttt ttttagcatc ttcaatggct ttctttctct catatgacca
    15061 atttattggt ctttttcttt ttgttgctct ctttttatct agcttaatta atcgtttata
    15121 aagaataccc ttttctttaa gagctataca gtactttctt ccttgaaaat aaggctctaa
    15181 aagcaccttt tcagcttttt caggaaatgt cttaggctca tatagcttag catataatgc
    15241 ttctgcttta tactcatcac ttccccataa taacttactt gatttttttg catattcaat
    15301 cttattttgt ttagatattt ttggattatc taaaaatcga taaaattgta ttggtgaaaa
    15361 tctagactga taatggtgac ttgtgactcc gtctttttcc ataaaccatt gcatagaaat
    15421 taacaagaca actttcttat tatcaatatt aggattcata cttcctaata ttgcagcatc
    15481 ttgtaatgtc tgagtatagg ctctcccaat tgcaaacacc ttatttttac tcctatttgt
    15541 attaaaatag tactttggat gttgttttgt tgaattacta agttctgatg accccaaaat
    15601 cataatatcc ttttctctta aaaaatgatt atttgctgta acccctttat cttttatatc
    15661 acttaaagta tcatccataa ttggaagtag atttttctcc ctcaaaagtt catcagtttt
    15721 agaatcaaga aatttgtcta acccaaataa aaatactacg cctattataa aaggtgtaat
    15781 aaagtatatc aattttctca tattttctct cctctaaaaa tattcaaatt tatgttatta
    15841 ttaatcatat tagttttgat atgtatattc aactacaaaa ttgtaataaa atcgtcaaaa
    15901 ctttttttga tactgttaca aaattgtaaa aaaaaagaaa aaaacgccaa aaataaattt
    15961 ctttttagca ctttttcata cttataattt gacattttaa cacttggcta agtatacacc
    16021 ttaccttaac ttaaaattaa atttatttaa gaatatatat tataaattct tatattttct
    16081 aatataaact atatactatc agctggataa acaatactac tttgtcttct tatctcatcc
    16141 ataatttcca ttactattat tgaattttca tgagaattta tatttgattc tattttattg
    16201 ttatttataa gacttataaa ctctacaagt tcataataca ttccttctat ttctctaggc
    16261 ttttcctctg ctttttcttt tggaatagat atatcttctt ctcttccatc tctgtattta
    16321 ataatagctt tttcaaatag attaagcttt tcaattatta tacttccctc ttctccttgt
    16381 atttcagatg gtatataaga atcagctact ttagaatact gaattatcgc atccatatca
    16441 tcatattgaa atacagctga accttctcca tccactccag tttgaagtat atgagaaact
    16501 gcctttacag tttttggagc gccaaataaa ttcaccatag gatgtataca atacactcct
    16561 atatccatta aagctccatt tgataactct tttttaaagg cattttctat aattccattt
    16621 ttatatttat catatcttga agaatactga caatagcttc caaaaaatct tcttattttc
    16681 cctattttat ataagttttc tttaactgct ttaaagtttg gaacacatgt aattctcata
    16741 gcttccataa gcaatacatt attatctttg gctgttttta tcattgattt gacttctttt
    16801 aaattagatg ctaacgactt ttcacaaagc acatgttttt tgtgttttaa acaagtaatg
    16861 gtttgctttg aatgtaatgc atttggagaa gctatgtata cagcatctat atccttcgat
    16921 ttagccattt gttctaaatc atcaaaaaaa atacttgctc catatagagt tccaaattct
    16981 ctagcttttt ctaaatttct agaatataca gcaaccaatt caaagtcttt aaccctagat
    17041 gcagctctta gaaatatatt tgctatattg cttgtcccaa caataccaaa tcttatttta
    17101 cccatcataa atcactcccc attttctaca ctttatttta attttaataa aaaactttac
    17161 aacttataga tatattataa aataatttta tcaaataaaa aagaaagcta attttaaatg
    17221 atttcaccat tttaacatag ctttctttta actacaatct aaattcaact catattttta
    17281 aagttatctt tctactatat ctttatttcc cctcaaaaat tttatagtaa gaatttcatc
    17341 cacaaacaat gttactaaaa gtatagttac aacaattggc caataaggta ttataagact
    17401 atataaacta tcaactatat acacttgaaa atatctaact ccaataaatg tcaatatagt
    17461 ccaatataca gaaaacttta gacatactat tcctctacta ttgtacttat aatctgaata
    17521 atcccaatag accttattaa aatatacttt cataagtatt cctgtaatat attctactac
    17581 tgtcggcact actagacata ataaaagcaa tgcaaatata ttattggggt caataaatct
    17641 tgagacctct attataatcg acattgcaat tgcatacatt ggtttaaatg gaccatttaa
    17701 aaatccatct tcttgaaaat gcccttgttt ataataacta tataagtttt caattatcca
    17761 acctataaaa ccatacaaga aaaaattaaa taaaaaatat aatagagaac ctacattatc
    17821 catgaatatt cctcctagct gtttatactt attttttctc atattcatgg atttattcta
    17881 aatcaaagaa attatattta atttatttaa ttaatacttt atattataga ttgtgacatt
    17941 ctttagagtt caaacttttt aggtattctc taacttccag taaattttca agtatcttat
    18001 gtgattgtga tttaatttgc tcatctggct cttttaaatc tggtacattt atacatctta
    18061 taccaccatt gtatgcagct tcaactccca taggcgaatc ctctatgaca atacaatttt
    18121 tgggattaac atttatcttt ttagctgctt ttaagaatat ctcagggttt ggtttagagt
    18181 ttacaacatc atctccacaa actatagcat caaagtaatc ttttaaattt gccttagcta
    18241 atctctttac tgctctttct ctctttgttg atgttgcaac agccattttg tatccatttt
    18301 cttttaaaaa agagattagt tcattgactc ctaatttgat aggagctccc ttcctctcca
    18361 taaattcaat catgttttta gttttttcgt catataaatc tattattggt acagaactat
    18421 cgtatatatc tgtaagacct tctattattc ctttacgatt tcttcccata actgatgtat
    18481 aaatttcctt agtcatagta tatccatact tttcaaacgt ctccatccaa aattcaagag
    18541 aaattctctc tgagtcaaac aaaactccat ccatgtcaaa aataattcct tctacttttt
    18601 gcatacaatt cctccaatat tttttgatat aattatatta cactttttat gattaatttt
    18661 acaaccctca atttatccaa ttctaatatt gtaaaattgt aattttcaaa agttacactt
    18721 tggtttactt taatatttga accaagcata taggatatcc acccacctat actgtcaaat
    18781 ccatcatgct ctatatcaag tccaaaatac ttattaatat agttgataga ttcagttcca
    18841 tcaactaagt aagaacctgc tcctattttc ttaatatgag tctcttcttt gtcaaattca
    18901 tcttgaattt ctccaactat ctcttctagt atatcttcta ttgttaccac accagatgta
    18961 ccaccatatt catccacaac tatagcaagt tgaagttttt cctttcttat tctctcaaga
    19021 gctttatcta tagttaaatt ttcagatata tatataatat ctctcaatat ttcttctagt
    19081 tcaattttat tttcatttat cttttgattg tataaatctc ttatatgtac aaaacctaat
    19141 atatcatctt tattttttct gcatactgga tatcttgtaa caccttcctc ttttaaaata
    19201 gctaatatct tttcttcaga atcactttca tatatacata ccatatcagt tctaggaacc
    19261 attatctctc taattttctt ttcttcaaat tcaaaaatat tatcaactaa tctttgctct
    19321 gattcatcta taagtccact tttataactt tcttctacta atagtcttat ttcgtcacct
    19381 gtatggggtt catcaacctc atcagcttgt gaatatccaa atggtttaag aaataaatca
    19441 gtacttaagt tgaaaaaata tattattgga tatgttaact tataaaatcc tactaataaa
    19501 aaagatgtac tcaacattat tttttctgta ttataaagtg ctaaagcttt aggaaccaac
    19561 tctcctatta caacctccat cattgttata attaaaaaag aaatggaaat tgatatagta
    19621 tatataatac tttcactaag attaaataca cttactattg gtaaaattaa ctctttaact
    19681 gtagattctc ccatccaacc taatgcaagt gaacaaagtg ttataccaag ttgacatgca
    19741 gaaagatagg aatttaagtt gtcttttatt attttacatc tttttgcatt attattacct
    19801 tcagttatca tggtatcaat cctagaacta cgaagtttta ccatagcaaa ttcacaagct
    19861 acaaaaaaag catttatcaa aacaagcatt actataagtg ttacttttaa taactcccgc
    19921 ataaagtatt ggttcttata atgtaagaaa tctataagaa ctcaaagttc cccccttata
    19981 tttataaatt taatttataa tatatattct atcatattta ttaatatatc ctactttatt
    20041 acaaataatt tttataaact aaataacccc ttttaatatt tatgactggc tatttgataa
    20101 atattaaagg ggttatttag ttatcatttt ttatttctaa aaatcttttc tcaaatgatt
    20161 tagttatcta tttatccaat tttttacata atttaatgat taaaa
//
LOCUS       DBGJFF010000067        19410 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.068, whole genome shotgun sequence.
ACCESSION   DBGJFF010000067 DBGJFF010000000
VERSION     DBGJFF010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19410
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.068"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(81..1949)
                     /locus_tag="ACOIAF_002386"
     CDS             complement(81..1949)
                     /locus_tag="ACOIAF_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp10"
                     /protein_id="HGZ2669394.1"
                     /translation="MSKRKSFIRTIAVSTMAVAVTGSATCAYAAPVLQGTKTYEKVNT
                     IDISVDSVENIVYSFQASIKVQGEVEVVDNEQKEKITWSDNIKSQIKSGNANATCRSE
                     YNKSSNTTTLDIYVTSNEDLLDGNRLNIGRISVKKSGSNSNADYKVLGKGTSDKPALK
                     IVTYNNKTVDYENISSDEGLIFTLINESEVKPIGGTGSSKNDPEKYKVEKSEALEYLL
                     NNIRINYSIVSKETQESGSNVILKLGLAQKTTKGRKATINKYVEVTLPKSLEYIVENE
                     LSKPDELPPDNGNGGGSNSGGSSSGGSSGGGNSSDSTSNVTVKKLKGTDRFETAIKIS
                     QSGWTKSDTVVIVNGEDKSMVDGLTATPLASVKNSPILLSSNEKLPQKTVEELKRLNP
                     SKVIVIGGNNSMPNSVVEAIKAVNSKISVQRIGGDTRYQTSINIAKEIDKTNNVSKLY
                     IGAGNGEADSLSIASLAGKEKTPIVLTQKDGVDNEAEQFIKSNKVSNIYFIGGVEKIS
                     NKAIEQVGKIANKDISNNRVAGQTRQETNAKVIDKFYSQSKLDGVVVANQDKLIDALA
                     VGPLAAKNNSPVILATNTLDKSQESSLKGKNSSKLFEVGGGIASSVIDKIKSLIEK"
     gene            complement(2159..8122)
                     /locus_tag="ACOIAF_002387"
     CDS             complement(2159..8122)
                     /locus_tag="ACOIAF_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M60 family metallopeptidase"
                     /protein_id="HGZ2669395.1"
                     /translation="MKKAISCVLAVSMCSAPLNVFAEPILEGKLRAVEKSVTEKLRGS
                     LEVDLNFSLPIKNSESESMTNMQLRLKDDSNNSGIIKLGEKDSGTVTVGTDNIEYTIK
                     KLSASRSEIKNKDENISYYNIVFNNLPVGKYNVEVSGAGFKNKEIKDIDISSYSQRVL
                     LSNRASVDNKNKPLNYELFLMGDVNGDGKVDKSDYNKVLENIDSNKREFDLNRDGKVD
                     IVDLDYVQKNLGQSEDVKSLESIVSTNPIVDTSKVELKGNSDVDINGNVENLFSGENN
                     GVTISSKNDEISEEKPAVMDIALSEALNMEVINIKAPEGTAPAKGIVEVTDKDGNVQQ
                     IPYENKEYMKNLKDEEQQEEVNNEQEEVNQEEVNNKDENINQTDNNSNNKDENNDKQQ
                     ENSDKQEVNSNTNQDKNNDKENSSMDKPNSDEETPKEEGNISKDDNSTTSTYSKSALE
                     TYADVQNSDIVINLGKQVAVKKITIKITATTANDRNLAEISKVEFLNNVYKEIPKPEM
                     NIPKINKISTSTAVGNEKISFYWNNEVNVSAYELILDKIDSKGKVLSTKKLQTSKNTI
                     EVRDIEAYGLYRVSIQSLNGDWSSGYKDATPENLDGVPENVDSNYKPIQFSPDSILEF
                     QVVPDTKPEPPEGINVKGKFKSLDVSWKNHKQAKDFDLYYKEKGSNGSWIKYNDKLIR
                     GTSSKIEGLKDNTTYEVRMTATNHLGTSGMSKTYIGTTESMDAPTTPNYRLINTPKEN
                     GGFGIPTNHIVDVTYPAGYDKSEYSDKNPFDKFNVVDGDFTTHWTFPTYNAAEGTNRG
                     PIITFDKSYTMDTFMATPRFDVSDKTINDFEVRYWDENGQLHNLGDVPFETKTDPVNN
                     NKYVMVRLKEPITATKIQVSVCSSYSYKSNISELKFYEYDSIEDDVRNLFMDDLQIEL
                     KEDVTQEKITELKDRLNTPDKASGEYHPYKTVIERELKLAQDLYNDRATLTDVTTVNQ
                     EINKLGKYKDKNGQYTVNSNNLGMQNDWQALGVAARAGDEITVYVGSKSGKTPKLIYT
                     QYYGESGAYKSGEINLKTGKNVIQLSKLHSLDIECGGSLYIRYAEDTPSGGDIKVRVA
                     GATKIPHLNLNGTINDKSPAGVSESKKKIKAYIEELAKYKDAVKGEPYYPGTNVGNSY
                     GYSEKTGVLNTTDIESDKVTLNVPATAVYQGISKGNTDLNTQVDRLYNSMLAWEQIMD
                     LVYSERGVFKTQDLDGNGTVDDNEFNLTKNDMAPKSRMNIKYQRMFIGAFMYASGLHV
                     GVEYGSVPGLMNGVPFQIDDSGKATGGNLFGWGIGHEIGHVTDIGKMTYSETSNNVLA
                     LLAQTFDDKTHSRLEGSTMDKIYEHVTSNSLGIPSNVFERLGMLWQLHLAYDDDFTGS
                     MLKNNSDADLSNDTFYAKISRKYRALSSSDPINSLPKDQMLVAMASSVVEKDLRDYFK
                     AWGVEITPELNSIMDSKNYEKESRKIQYVNDEARRKILNENIASMAKDTKVSASFSDG
                     IQSGSLVKSNKISIDLDVDKDKDKILGYEIIRSDGNYLDGENGTQVKYRTVGFVDAKG
                     NSKAKFTDNISPLNNRAFTYKVVAYDYHLNPTEEFEVGTVKLSDEGKINKSAWSFITN
                     TVSDGDVRTENDSHGPLQNPEIDNIKDNDASTTYKGKIISKAEWNKDPQKDPDINVEE
                     NPYIIVDMKETLPIVGLKYTKPKTEARKFSLKGLFNFRKDTSTTYNPLTNYKIQVSND
                     KKNWEDVSQGTFEYGQDLVGGGKDQDNEARVTFNKDGKLWTYQARYVKLISNNKSNIE
                     VAELDIIGPPGDNIEIGTMNNADQTRTNGIGKLAEDYVYQSDDSNTSEDETGKIPAGS
                     IVITGEYSGNPAFNLPLLIDKNNKTISGEALLFAEVPEKGELGEISKGTWVYYMPKEN
                     FDSLSDKVKAELYRYNDLQGDTPVGQRFVSDTMYVPVGAKNYDELKTISLTDSNSKAR
                     KATRNSIDISNKKVITVSNKVKGNIVRDK"
     regulatory      complement(8246..8331)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(8503..9906)
                     /locus_tag="ACOIAF_002388"
     CDS             complement(8503..9906)
                     /locus_tag="ACOIAF_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp9"
                     /protein_id="HGZ2669396.1"
                     /translation="MKNSKKILAIGLTLFLVMVNTPMVNALTSVEQIKGNDRYETAAK
                     IADKQNYNTAILINSDNSLADGLSASGLAGALNAPILMTKQNQIPNTTMERLNKAKTV
                     YIIGSESTISKNVENQLLSKKKVVQRIFGENRFDTSIKIAEKIKEIKPIDKVIIANGF
                     TGEADAISASPVAARDGVPIILTDGNSVGFDTTGLKSYALGSSEIISDELVKSTNSIR
                     LGGTDRFETNKIVIQEFYKNSKEFYLSKGLQLTDALVASTIAKNAPVVLVENGSNKSI
                     LSGADKLTVLGGINQNVIKQCINQASPNQQGLYYNPNDRAFKERIKGKVYALTKQYRK
                     ENGVRALSVASRLEGLANDWSNLMANKKTLSHTINGKNSYSTFLKYLDWSEIKPGYIA
                     VQGENIIKYKIPDKPVYTNRDADDIGNFIFNEWKTNPEEGTNMLHKGYEIMGFGIAIT
                     GDKNLYATHEFYGRYKE"
     gene            complement(10368..12263)
                     /locus_tag="ACOIAF_002389"
     CDS             complement(10368..12263)
                     /locus_tag="ACOIAF_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp8"
                     /protein_id="HGZ2669397.1"
                     /translation="MLSNKKRSMAIVMAGATVMSAAAPIFADNTVTENVDKNYTVSAK
                     DSAKLIEEVRKALEVKFEDTKAGANVNDRVYDIKVDNVNLTNATQLQNKINSLTEGQS
                     LKVTIQDKGHQVLGGKVVDYKIENYKTAQEIVDAVNAYNATLAEDSDNKLTATIKSTN
                     TVEVKRAKDSANVITLNVEDQHLDFSKVITSEEGTFEGYEKSYSDIDSKELHTITVKN
                     ADLQDISAEELFDGIRLTTLGREIVNKVKNGYALTFENEAILTQEQEDSDDKDKPEKS
                     SFDIVLSKANEKPETISVSSKNHKLVRDLHKVLTDVKDGKELKVEVLSGDSRFTTAVE
                     VSKERFKDGEAEAIILVGEDAIVDGLASAPLASQKNAPILLSKKDSLPSEIEAEILRV
                     LGSNLSSKKIYIVGGESKVSKETEEKLSKLGVSKVERVSGEDRFETSLEIAKQLKDTF
                     KTAFVVGGNGEADAMSISARAAQFGAPIIVTGNELDANAEKLLKGKELEIVGGENSVS
                     KEVEDKLVDIDLNNKVERLAGENRKDTNAKVINKYYAGATKAYVAKDGYVGGNGQLVD
                     ALTAAPLAASSKAPIVLTTEELSKSQEEVVELRLKNATKLVQIGEGIAKNAIEKIAEK
                     INLFTKN"
     gene            complement(12890..13033)
                     /gene="scfA"
                     /locus_tag="ACOIAF_002390"
     CDS             complement(12890..13033)
                     /gene="scfA"
                     /locus_tag="ACOIAF_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HGZ2669398.1"
                     /translation="MENKHVKTLSQATLKQSAAKGGCGECQTSCQSACKTSCTVANQE
                     CER"
     gene            complement(13099..13461)
                     /locus_tag="ACOIAF_002391"
     CDS             complement(13099..13461)
                     /locus_tag="ACOIAF_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HGZ2669399.1"
                     /translation="MGKSNYIFKGLGYAYIITLAVLLVYNLFLTFTDIGGDNITMVSS
                     FITTISAAIGGFYTSKHMKEKGLMYGLLVGLLYIVCIFLTVFLAQEKFVFEVGMIYKL
                     LLISAAGGIGGVLGVNFK"
     gene            complement(13519..13812)
                     /gene="yajC"
                     /locus_tag="ACOIAF_002392"
     CDS             complement(13519..13812)
                     /gene="yajC"
                     /locus_tag="ACOIAF_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HGZ2669400.1"
                     /translation="MPQQQIIMSIGLWVVVIAIFYFLMIRPQKKKDKQLKEMRSSLSV
                     GDKVITIGGIVANVAKVEDDVVILELGPNRTKVPFEKWAIGTVKSKKEEIEED"
     gene            complement(13946..15067)
                     /gene="tgt"
                     /locus_tag="ACOIAF_002393"
     CDS             complement(13946..15067)
                     /gene="tgt"
                     /locus_tag="ACOIAF_002393"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HGZ2669401.1"
                     /translation="MYAVRYELIKTCKQSGARLGRLHTPHGIIETPIFMPVGTQATVK
                     SMTPEELKEIGSQIILSNTYHLYMRPGHELIKRAGGLHKFMNWDKPILTDSGGFQVFS
                     LGPLRKIKEEGVEFRSHLDGSKHFLTPEKAMEIQNALGSDIMMAFDECAPYPSDREYV
                     KNSLERTTRWLKRCKDAHNNTDKQALFGIIQGGMYKDLREQSAKEITNIDLPGYAIGG
                     LSVGEPKPLMYDVLEHTTPLMPKDKPRYLMGVGSPDDLVEGVIRGVDMFDCVLPTRIA
                     RNGTAMTSQGKVVVRNATYAEDFTPLDPECDCYACKNYSRAYIRHLIKANEILGARLI
                     TTHNLHFLLNLMKQIRQAIMEDRLLDFRNEFFAKYGYEI"
     gene            complement(15146..15631)
                     /locus_tag="ACOIAF_002394"
     CDS             complement(15146..15631)
                     /locus_tag="ACOIAF_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HGZ2669402.1"
                     /translation="MHSLQKQKKNNPILKIAFIFERVLAVVVLIAVFLGTIDVLRLMW
                     GAYIVDFQNPVQYSQLNDFLAQILLLVIGVELVVMLSLHIPGAFIEALLYAIARKMLL
                     LPKNEGMIDVLIGVIAIAGLFAIKKFLVTKDKSALNIMSIHDEEAIKEQQNKEDEDKS
                     I"
     gene            complement(15643..16668)
                     /gene="queA"
                     /locus_tag="ACOIAF_002395"
     CDS             complement(15643..16668)
                     /gene="queA"
                     /locus_tag="ACOIAF_002395"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HGZ2669403.1"
                     /translation="MKTSDFKFDLPQELIAQVPIEDRASSRLMVLDKETGNIEHKVFR
                     DIIEYLNPGDCLVLNNTRVIPARLIGEKLETGGKIEFLLLKRTEEDTWQALVKPGKRA
                     KVGTKFSFGNGKLIGEVVDLSDEGSRIIKFHYDGIFEEILDELGNMPLPPYITARLDE
                     KERYQTVYSKHNGSAAAPTAGLHFTEELLNKIKEKGVDIAFVTLHVGLGTFRPVKVED
                     VLNHKMHSEYYMVSQEAADKINRAKENGKNVICVGTTSCRTIESACNEDGKMKETSGW
                     TEIFIYPGYKFKVLDKLITNFHLPESTLIMLVSAICGKDNVLNAYNEAVKERYRFFSF
                     GDAMIIK"
     gene            complement(16986..18005)
                     /gene="ruvB"
                     /locus_tag="ACOIAF_002396"
     CDS             complement(16986..18005)
                     /gene="ruvB"
                     /locus_tag="ACOIAF_002396"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HGZ2669404.1"
                     /translation="MQGFEDENRIITSTMKMEDIDIENSLRPKTLEDYLGQEKSKEQL
                     SIFIEAAKSRNEQLDHVLLYGPPGLGKTTLASIIANEMGVNLRITSGPAIERAGDLAA
                     ILTNLNENDVLFIDEIHRINRSVEEVLYPAMEDFCLDIIIGKGPSARSIRLDLPKFTL
                     IGATTRAGMLTNPLRDRFGVICKLDYYTVDELSKIVLRSSSILDAEIQSNGALELAKR
                     SRGTPRIANRLLKRVRDFAQVRADGKITDKVAKDALELLGVDSLGLDFVDEKLLMTII
                     EKFRGGPVGLDTLAASIGEDRNTIEDVYEPYLLQLGFINRGPRGRVAMPLAYEHLKIP
                     YPNEK"
     gene            complement(18112..18714)
                     /gene="ruvA"
                     /locus_tag="ACOIAF_002397"
     CDS             complement(18112..18714)
                     /gene="ruvA"
                     /locus_tag="ACOIAF_002397"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HGZ2669405.1"
                     /translation="MYSYIKGTVEEIYIDSIVVENNGIGYKINVSSNTIMNLQVGEAT
                     KIYTKLIVREDDMSLCGFVSREELKMFELLTSVSKIGPKVALSILSFASPAQLGAYIL
                     SEDIGKLSKAPGVGKKTAERIVLELKDKVDKNNIEFEPTLLSQKPTLISQDESVDALV
                     ALGYTLSESKEAVQKCKKDGMNTEAIIKKALTYIMSKSLK"
     gene            complement(18707..19216)
                     /gene="ruvC"
                     /locus_tag="ACOIAF_002398"
     CDS             complement(18707..19216)
                     /gene="ruvC"
                     /locus_tag="ACOIAF_002398"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HGZ2669406.1"
                     /translation="MIILGIDPGIAIVGYGIIEYKNSKFKVIDYGAVTTPAHMNISRR
                     LELVYKGIDTIVKNYNIDEVGMEELFFNKNVKTAITVAQARGVTMLACAHNGKPVYEY
                     TPLQVKQGVVGYGRADKAQVQQMVTSFLSLKKVPKPDDVADALAVAICHAHSNKLEKT
                     LKNIGGKYV"
BASE COUNT         5602 a         3615 c         2168 g         8025 t
ORIGIN      
        1 ataatgaaaa taaaataaga atgcatttaa atatataact ttatatctaa atacattctt
       61 attttctatt tacttaatat ttatttttca attaaactct taatcttgtc tattacagat
      121 gaggctattc ctccacctac ttcaaacaac tttgatgaat ttttaccttt taatgacgat
      181 tcttgtgatt tatctaacgt atttgtagca agtataactg gtgaattgtt tttagctgct
      241 aatggtccaa ctgctaaagc atctataagt ttatcttgat ttgcaactac aactccatct
      301 agcttagatt gagagtagaa tttatctatt actttagcat tcgtctcttg tctagtttga
      361 cctgccaccc tattgtttga tatatctttg tttgctattt ttccaacttg ttcaatagcc
      421 ttgttagata ttttttctac tccaccaata aaatatatat ttgacacctt atttgattta
      481 ataaattgtt cagcttcatt atctacacca tctttttgtg taagtactat tggagttttt
      541 tctttaccag ccagtgatgc tattgataat gaatcagctt ctccatttcc tgctcctata
      601 tacaacttgc ttacattatt cgtcttatct atttcttttg ctatattaat agaagtttga
      661 tatcttgtat ctccacctat tctttgtact gatatttttg aatttacagc tttgatagct
      721 tcaacaactg aatttggcat tgagttgttt ccacctatca caattacttt tgatggattt
      781 agccttttca actcttctac agttttttga ggaagctttt catttgatga taaaagaatc
      841 ggtgagtttt ttacactagc aagtggtgta gctgtaagcc catccaccat acttttatct
      901 tctccattta ctattacaac tgtatctgat tttgtccaac cactttgaga tattttgata
      961 gctgtctcaa atctatcagt accttttaat tttttgactg tgacattaga tgtagaatca
     1021 cttgaatttc ctcctccaga gcttccacca ctagacgagc cgcctgaatt acttcctcca
     1081 ccattgccat tatctggtgg taattcatcc ggttttgata attcattttc aactatgtat
     1141 tctaaacttt ttggaagtgt aacttcaaca tatttgttta ttgtagcttt tcttccctta
     1201 gttgtttttt gtgctaatcc aagttttaat attacatttg aaccactttc ttgtgtttct
     1261 ttactaacaa tagaatagtt tatcctaata ttgtttaata aatattcaag tgcttcactt
     1321 ttctctacct tatacttttc agggtcattt ttactagaac cagttccacc aattggtttt
     1381 acttcactct cattaataag ggtaaatatt aatccttcat cagagcttat attttcataa
     1441 tccactgttt tgttgttata tgttacaatt tttaaagctg gtttatcgct tgttcctttt
     1501 cctaaaactt tatagtcagc atttgagtta ctgccagatt tttttacaga tattcttcct
     1561 atattcaatc tatttccgtc tagtaagtct tcatttgatg taacatatat atctaatgta
     1621 gttgtattac ttgatttgtt gtattctgat ctacaagtag catttgcatt tccacttttt
     1681 atctgagatt ttatattatc agaccaagta atcttttctt tctgctcatt atctactact
     1741 tcaacctcac cttgtacttt tatactggct tgaaatgaat atactatgtt ttctactgag
     1801 tccacagata tatctatagt atttactttc tcatacgttt ttgttccttg taaaactgga
     1861 gctgcatatg cacatgtagc actaccagtt acagctactg ccatagttga tactgctata
     1921 gttcttataa aagattttct tttactcatt aacacccgtc cttatctatc attaactaag
     1981 tggataaatt tacttatcca cctagctttt atatttaatt tataaattac cctaaactta
     2041 tagttacatt tatatacttt acttttaata tctactattt taaaactatt ctagtaccta
     2101 atcttattag atatacaatg ttagtgttag tatttttacg tttttagagt tccacatact
     2161 atttatctct aacaatatta ccctttactt tattgcttac agttataacc ttcttattac
     2221 tgatatcaat agaatttctt gtagcttttc tagctttact attactatca gtaagactta
     2281 ttgtctttaa ctcatcataa ttcttagctc ctacaggtac atacattgta tcacttacaa
     2341 atctttgacc tactggagta tctccttgaa ggtcattgta tctatatagt tcagccttaa
     2401 ccttatcact taaagaatca aagttttctt ttggcatata gtaaacccat gtacctttac
     2461 tgatttcacc taattcacct ttttctggaa cttctgcaaa taaaagtgct tctccagata
     2521 tagtcttatt gtttttatct ataagtagtg gtaaattaaa tgctggattt ccactgtatt
     2581 cacctgtaat aactattgaa cctgctggaa tcttacctgt ttcatcttca cttgtgttag
     2641 agtcatcact ttgatataca taatcttctg ctaactttcc aattccatta gtacgagttt
     2701 ggtctgcatt attcattgtg ccaatttcta tattatcacc tggaggtcct attatatcta
     2761 attctgcaac ttctatatta gacttattat ttgagattag ttttacatat ctagcttggt
     2821 atgtccaaag tttaccatct ttattaaatg taactctagc ttcattatct tggtcttttc
     2881 ctccaccaac taaatcttgt ccatattcaa aagttccttg acttacatct tcccagttct
     2941 ttttatcatt acttacttgt atcttataat tagtcaaagg attgtatgtt gtgctagtat
     3001 cctttctaaa gttaaataat ccttttaatg agaactttct agcttctgtc tttggttttg
     3061 tatattttag tcctactata ggtaatgttt ctttcatgtc tactattata tatggatttt
     3121 cttctacatt tatatcaggg tctttttgag ggtctttgtt ccactctgct ttacttatta
     3181 tctttccttt atatgtagta cttgcatcat tatcttttat attatcaatt tcaggatttt
     3241 gtaatggtcc atgtgagtca ttttcagttc taacatcacc gtcagaaaca gtatttgtta
     3301 tgaatgacca tgctgattta tttattttcc cttcatctga taattttaca gtaccaactt
     3361 caaattcttc tgttggattt aggtgataat catatgcaac taccttatat gtaaatgctc
     3421 tgttattaag aggagatata ttatcagtaa atttagcttt actatttcct tttgcatcca
     3481 caaatccaac tgttctgtat tttacctgtg ttccattttc accatctaaa taatttccat
     3541 cacttcttat tatttcgtat cctagtatct tatctttatc cttatctaca tctaggtcta
     3601 tagatatttt attagattta actagagaac cactctgtat tccatcagaa aaactagcac
     3661 ttactttagt atcttttgcc atactagcta tattttcatt taaaatcttt cttctagctt
     3721 cgtcatttac atattgtatc tttcttgatt ctttttcgta gttcttacta tccattatac
     3781 tgtttaattc tggtgtaatt tctactcccc atgccttaaa gtaatctctt aaatcttttt
     3841 caacaacact tgatgccatt gctactagca tttggtcttt tggtaaacta ttaattgggt
     3901 cactacttga aagtgctctg tattttcttg aaatctttgc atagaatgtg tcattactta
     3961 aatcagcatc tgagttattc tttaacattg aacccgtaaa atcatcatca taagctaaat
     4021 gtaattgcca taacattcca agcctttcaa atacattgct tggtattcct aagctatttg
     4081 aagttacatg ctcatatatc ttatccattg ttgaaccttc aagtcttgaa tgtgtcttat
     4141 catcaaaggt ctgagcaagt aaagcaagta cattgttact agtttcgcta tatgtcatct
     4201 ttccaatatc tgtaacatgt cctatctcat gacctattcc ccatccaaat aagtttcctc
     4261 ctgtagcttt tccactatca tctatttgga aaggtactcc attcataagc cctggaactg
     4321 aaccgtattc tactcctaca tgtaatccag aagcatacat aaatgctcca ataaacattc
     4381 tttggtattt tatgttcata cgagattttg gagccatatc atttttagtt aagttaaatt
     4441 cattatcatc tacagttcca ttaccatcta aatcttgtgt tttaaatact cctctttctg
     4501 agtaaactaa atccattatt tgttcccatg caagcatact attatataat ctatctactt
     4561 gtgtatttaa atcagtattt ccttttgata ttccttgata tacagctgtt gcaggaacat
     4621 ttaatgttac tttatcactt tcaatatctg tagtgtttaa cacaccagtt ttttctgaat
     4681 atccatatga attaccaaca tttgttcctg gataatatgg ttctcctttt acagcatctt
     4741 tatatttagc taattcttct atatatgctt ttatcttttt cttagattca gaaactcccg
     4801 caggactctt atcatttatt gttccattta gattaagatg tggtatctta gtagctcctg
     4861 ctacacgtac ttttatatct cctccacttg gagtatcttc agcatatctt atgtataaac
     4921 ttcctccaca ttctatatct aaactatgaa gtttgcttaa ttgaattaca ttttttccag
     4981 ttttaagatt tatttctcca ctcttatatg caccagattc tccatagtac tgtgtatata
     5041 tcaacttagg agtttttcca cttttacttc caacatatac tgtgatttca tctcctgctc
     5101 tagcagctac tcctaatgcc tgccagtcat tttgcatacc taaattattg gaatttacag
     5161 tatactgtcc gtttttatct ttatattttc ctaatttatt tatttcttga tttacagtag
     5221 ttacatcagt aagtgtggct ctatcgttat ataagtcttg agctaatttt aattctctct
     5281 caataactgt cttatatgga tgatattctc cactagcttt atctggagta tttaatctat
     5341 cttttaattc agttattttt tcttgagtta catcttcttt taattcaatt tgtaggtcat
     5401 ccataaatag atttcttaca tcatcttcta tactgtcata ctcataaaac ttaagttctg
     5461 atatattaga cttatatgaa taggatgagc aaacacttac ttgtatcttg gtagcagtta
     5521 ttggttcttt taatctaacc attacatact tattattatt tacagggtct gtctttgttt
     5581 caaaaggaac atcccccaga ttgtgtaatt gaccattttc atcccaatat cgtacctcaa
     5641 agtcatttat tgtcttatca gaaacatcaa aacgaggtgt agccataaaa gtatccatag
     5701 tataagattt atcaaaagtt attattggtc ctctattcgt tccctcagct gcattatatg
     5761 ttgggaatgt ccaatgtgtt gtaaagtccc catctactac attgaattta tcaaatggat
     5821 ttttatcact atattcactt ttgtcatatc ctgctggata agtaacatct acaatgtgat
     5881 ttgttggtat tccaaatcca ccattctctt taggagtatt aattaatcta tagttaggtg
     5941 ttgttggtgc atccatgctt tcagttgtac ctatatatgt tttagacatt ccactagttc
     6001 ccaagtggtt agtagctgtc attctaactt catatgttgt attatcctta agaccttcta
     6061 tctttgaact tgtcccacga ataagcttat cattgtattt tatccaagac ccattagaac
     6121 ctttttcttt atagtacaag tcaaaatctt tagcttgttt atgatttttc cagcttacat
     6181 caagagattt aaatttacct ttaacattta taccttctgg tggttctggt tttgtgtctg
     6241 gaacaacttg aaattctaat atactatctg ggctaaattg tataggttta tagtttgaat
     6301 ctacattttc tggtactccg tctaagtttt ctggtgttgc atctttataa ccactagacc
     6361 aatctccatt taaagattgt atgctaaccc tatatagtcc atatgcttct atatctctaa
     6421 cttctattgt atttttacta gtttgtaatt ttttagtaga taatactttt cccttactat
     6481 caattttgtc tagtataagt tcgtatgctg atacatttac ttcattattc caataaaaag
     6541 atattttttc atttccaaca gcagtgctag tcgatatttt atttatttta ggtatgttca
     6601 tctctggttt tggtatttct ttatatacat tgtttaaaaa ttcaactttt gatatttctg
     6661 caagatttct atcatttgct gttgtagctg ttatttttat agttatcttt tttactgcta
     6721 cttgtttacc aagatttatt actatatcgc tattttgaac atctgcatat gtttctaatg
     6781 ctgacttact atatgtagat gttgtagagt tatcatcttt actgatatta ccttcttctt
     6841 ttggagtctc ttcatctgaa ttaggtttat ccattgatga attttcttta tcattatttt
     6901 tatcttgatt ggtattagag tttacttctt gtttatcgct attttcctgt tgtttatcat
     6961 tattttcatc tttattattt gagttattat cagtctggtt tatattttca tctttattat
     7021 ttacttcttc ttgatttact tcttcttgtt cgttatttac ttcctcttgt tgctcctcat
     7081 cttttaagtt tttcatatat tctttgtttt cataaggaat ctgttgaaca ttaccatctt
     7141 tgtcagtaac ctcaactatt ccttttgctg gagcagtacc ttctggagct tttatattga
     7201 tgacctccat attaagtgct tctgataaag ctatatccat tactgctggt ttttcctcag
     7261 atatttcatc attttttgaa gatattgtaa ctccattatt ttctccacta aataaatttt
     7321 ctacattacc attaatatct acatcactat ttccttttaa ttcaacctta cttgtatcta
     7381 ctataggatt ggtagatact atactttcta ggctttttac atcttcactt tgtcctaaat
     7441 ttttctgtac ataatctaag tcaacaatat ctacctttcc atctctattt aaatcaaatt
     7501 ctcttttatt tgaatctata ttctctaaaa ccttattata atcactctta tctactttac
     7561 cgtctccatt tacatcaccc attaagaata attcataatt taaaggttta tttttattat
     7621 ctacacttgc tctattgctt aataagactc tttgtgaata acttgatata tcaatgtctt
     7681 taatttcttt gttcttaaat ccagctccag agacttctac attgtatttt ccaacaggta
     7741 aattattaaa tacaatatta taatatgata tattctcatc tttatttttt atttcacttc
     7801 tacttgcaga taattttttt atagtatatt ctatattatc tgtcccaaca gttactgttc
     7861 ctgaatcttt ttctccaagc tttattattc cactattgtt actatcatct tttaatcgta
     7921 actgcatatt agtcatactt tctgactcgc tattttttat tggcagtgaa aaatttaaat
     7981 ctacttcaag actccccctt aacttttctg taactgattt ttcaactgcc cttagcttcc
     8041 cctctaatat aggttctgca aaaacgttta gtggtgcact acacatagat actgctagta
     8101 cacaagatat tgcctttttc atgaaataac aatcctccct taataaatat ctaatttttc
     8161 atgaatttaa atactataag tttcagacaa taaaaaaaga cactcaaaga gaatcttaat
     8221 tataaaataa aaagacttta ctctttttag caactggctg atatggcttt aaaagcttta
     8281 atccctatag ttttgcgtca ttagatttcc ctaattttgc catttaattt tcactacttt
     8341 cttacccatt ttatactata tttaaacttt ttaatacaat ttttttacat taaattataa
     8401 aaaaataaca tttatattaa tttattaata taaatgttat tttccattat aatttaatta
     8461 tttatatagc ttccttacaa gcataatttt gtgtatattt tcttattctt tatatcttcc
     8521 ataaaattca tgtgtagcat atagattctt atctcctgtt atagctatac caaatcccat
     8581 tatttcatac cctttatgca acatattagt gccttcttct ggatttgtct tccactcatt
     8641 aaaaataaag tttcctatat catctgcatc tctatttgta tatacaggtt tatctggaat
     8701 tttatattta attatatttt caccttgaac tgctatatat ccaggtttaa tctcactcca
     8761 atctaagtat ttcaaaaaag tagaatatga attttttcca tttatcgtat gagataaagt
     8821 cttcttattt gccattaaat tagaccaatc atttgctaga ccctctaatc tgcttgctac
     8881 agataacgct cttactccat tctcttttct atactgcttt gtaagagcat atactttacc
     8941 ctttattctc tctttaaatg ctctatcatt tggattgtaa tataaccctt gttgatttgg
     9001 tgaagcttga tttatacatt gcttgataac gttctgattt atgcctccta gtacagtcaa
     9061 tttgtctgca ccacttaaaa tactcttgtt actgccattt tcaactaaaa ctacaggagc
     9121 attttttgct attgtagagg caaccaaagc gtctgttaat tgtaagcctt tacttagata
     9181 aaattcttta ctatttttat aaaactcttg tataactatc ttattagttt caaatctatc
     9241 tgtgccaccc aatctaatag aatttgtgct cttaactaat tcgtcgctga ttatttcact
     9301 agaaccaagc gcataactct ttaagcctgt cgtgtcaaat cccacactat ttccatctgt
     9361 aagtattata ggtactccat ctctagcagc tactggtgat gcacttatcg catctgcttc
     9421 tcctgtaaat ccattggcta taattacttt atctattggc ttaatttctt tgattttttc
     9481 agctattttt atacttgtat caaatctatt ttcaccaaag attctttgta caaccttctt
     9541 tttggatagt aactgattct caacattttt acttattgtt gattctgagc ctattatata
     9601 cacagtcttt gctttgttta atctttccat agttgtgttt ggaatctggt tttgttttgt
     9661 catcaaaata ggcgcattta aggctccagc taaaccactt gcacttagac catctgctaa
     9721 gctattatct gagttgatta gtattgctgt attgtagttt tgcttatctg ctatttttgc
     9781 tgctgtctcg tatctgtcat tcccttttat ttgctcaact gatgtcaacg catttaccat
     9841 aggagtattt accattacta aaaatagtgt aagtcctata gctaatatct ttttactatt
     9901 cttcatatta ttttctaccc ccaatgtact tttcatattt tttattagta ctcatactta
     9961 tttttaatca aaagttacaa ttttgttacc atattttata tatgatacca tagttatcca
    10021 tacaactcaa acaatatacc taaagttttt attctctata aattcaaaac attataattt
    10081 attttattta attaagttat aagttcaaaa taatttatgt taataagatg tttaatcatt
    10141 ataagaagta tctttttata ataatttttg caatatgaat ttctcaacat tataatcaaa
    10201 aaataaaata aggtatctca atctaattct aaatgagaca ccttattttt attattttat
    10261 cttgttattt gtatatttta taatatctac actatgtctt acatattaac gcattgtata
    10321 atcatatatt atctaaataa acttttaata ttaattccta tatcttatta gttcttagta
    10381 aataagttta ttttttctgc tattttttca atagcgtttt tagctattcc ttcacctatt
    10441 tgtactaact tagtagcatt cttaagtctt aactcaacta cttcttcttg tgatttagaa
    10501 agttcttctg tagttaatac tattggagcc tttgaactag ctgcaagtgg tgctgctgta
    10561 agtgcatcaa ctaattgtcc atttccacct acataaccat cttttgctac atatgcttta
    10621 gttgcacctg catagtattt attgattact ttagcattag tatcttttct attttctcca
    10681 gctaatcttt caactttatt atttaaatct atatcaacta atttgtcttc aacttctttt
    10741 gatacagaat tttctccacc cactatttct aattcttttc cttttaataa tttttcagca
    10801 tttgcatcta attcgttacc tgtaactatt attggagcac caaattgagc agctctagct
    10861 gatatactca tagcatcagc ttctccattt ccacctacta caaaagcagt cttaaatgta
    10921 tcttttaatt gttttgctat ttctaaagaa gtttcaaatc tatcttctcc agaaactctc
    10981 tcaactttac ttacacctaa tttagaaagt ttttcttcag tttcttttga tactttagat
    11041 tctccaccta ctatatatat tttcttagaa gatagattac ttccaagtac tcttaatatt
    11101 tcagcttcta tttctgatgg tagtgaatct tttttagata ataatattgg tgcatttttt
    11161 tgagaggcaa gtggtgctga tgctaatcca tcaactatag catcttctcc aactaaaatt
    11221 atagcttctg cttctccatc tttgaatctc tctttactaa cttctactgc tgttgtaaat
    11281 cttgaatcac cagataaaac ttctactttt aattcttttc catcttttac atcagttaat
    11341 actttgtgta agtctctaac taacttatgg tttttacttg aaacacttat tgtttctggc
    11401 ttttcatttg ccttgcttaa aactatatca aagcttgatt tttctggttt atctttatca
    11461 tcactatctt cttgttcttg agtaaggatt gcttcatttt caaatgttaa agcatatcca
    11521 tttttaactt tattaactat ttctcttcct aatgtagtta atctaattcc atcaaataat
    11581 tcttctgctg atatgtcttg taaatctgcg tttttaactg ttatcgtatg taattctttt
    11641 gaatctatgt cactataact cttttcataa ccttcaaaag taccttcttc tgatgttatc
    11701 acttttgaga aatctaaatg ttggtcttct acatttaatg taataacatt tgctgaatct
    11761 tttgctcttt ttacttcaac tgtatttgta gattttatag ttgctgttaa cttgttatca
    11821 gaatcttctg ctaaagttgc attgtaagca ttaactgcat ctactatttc ttgagcagtt
    11881 ttgtagtttt caattttata gtcaactact tttccaccta acacttgatg tcctttatct
    11941 tgaatagtaa cttttaaact ttgcccttct gttaaagagt ttattttatt ttgtaattga
    12001 gttgcattag ttaagtttac attgtctact tttatatcgt aaactctatc atttacattt
    12061 gctcctgctt ttgtatcttc aaacttaact tctaatgctt ttctaacctc ttcgattagt
    12121 ttagcagaat ctttcgcact tactgtgtaa tttttatcaa cattttctgt cacagtattg
    12181 tctgcgaata ttggtgctgc tgcactcata actgtagcac ctgccattac tattgccata
    12241 cttctctttt tgttgcttaa catatttatg tcctccgaat attatttttt attttttata
    12301 ttcgtatttc ccacacataa ataatattat atataatatt atttttcaat atattttatg
    12361 tgtttacata atttactaac acaaacacta ataatatctt cttgaacccc ttccccctac
    12421 tgagctctct ataattatgc tcaaagatag aaatccctat tttatagtga ttttctacta
    12481 aacaatcata tactaacaaa agtattcaaa ctccattttt atatactact atgcactaat
    12541 ttttgcttta ctcttattac ttaaatatat atcactaaat taatatctat ctccatatat
    12601 taaaatatat aacatattat aaaaatcatc ataaattaga gtttaaaatt aaaaattatt
    12661 ctaatacatg taattgataa aaaataaaat ataagagtag ctactttaaa ataaatgctc
    12721 ttatatgatt agatattttt gcataattaa acgataagaa atcaaattta attctacata
    12781 aatagaatat atatttttat ataataattg aaaataaaag aggatacctc ataatgataa
    12841 aagaatcact ttgagatacc ctcttacaaa caactaaatc aataattttc tatctttcac
    12901 actcttgatt tgcaactgta caagaagttt tacaagctga ttgacaagaa gtttgacact
    12961 ctccacatcc accttttgca gcactttgct ttaaagtagc ttgagataaa gtttttacat
    13021 gtttattttc cattttcaac gcctccattt ctaattgttt cctattgatt atatcataaa
    13081 actactttaa tataaactct atttaaagtt gacacccaat acgcctccaa tgcctcctgc
    13141 tgctgatatt agcaataact tataaatcat ccctacttca aatacaaact tttcttgtgc
    13201 taaaaacaca gtcaaaaaaa tacatactat gtacaaaagt cctactaaaa gcccatacat
    13261 aagtcctttt tctttcatgt gttttgaagt gtaaaatcct cctatagctg cagaaatagt
    13321 agttataaat gatgaaacca tagttatatt atctccacca atatctgtaa atgtcaaaaa
    13381 taagttatac actaataaaa cagccaaagt tattatatat gcatatccta gacctttaaa
    13441 aatataatta gattttccca taaaaaaacc tcctctcaaa ttaatatata ttcgagaaaa
    13501 ggcttttaaa ttactaattt agtcttcttc tatttcttct tttttagatt taacagttcc
    13561 tatagcccat ttttcaaatg gaaccttagt tctatttggt cctaattcta atattacaac
    13621 atcatcttca actttagcta cattagctac tattccacct attgtaatga ccttatctcc
    13681 tacacttagg ctacttctca tttcttttaa ttgtttgtct tttttctttt gaggtctaat
    13741 cattaggaaa taaaagattg cgattactac tacccataga cctatactca taattatttg
    13801 ttgctgtggc attaattttt cactcctttg taaattatac tcttttgtaa atcataataa
    13861 atgtttatat aacaatttac tttctctatc tttgagtact attgaatatt atatattaaa
    13921 atgattataa ttaatagtgc aaaacctata tttcatatcc atattttgca aaaaattcat
    13981 ttctaaaatc aagtaatcta tcttccatta tcgcttgtct tatttgtttc attaaattta
    14041 ataaaaagtg aagattatga gtagttatta atcttgcacc cagtatttca tttgctttta
    14101 taaggtgtct tatataagct cttgaatagt ttttacaagc ataacaatca cattcagggt
    14161 caagtggggt aaagtcttca gcataagttg catttctaac tactacttta ccttggctag
    14221 tcattgcagt tccatttcta gcgatacgag taggtaggac acaatcaaac atgtctactc
    14281 ctcttataac accttctact aagtcatctg gactaccaac tcccattagg tatcttggtt
    14341 tatcctttgg cataagtgga gttgtatgct ctagcacatc atacataagt ggttttggtt
    14401 ctccaacact aagtccacca atagcatatc ctggtaaatc aatattagtt atttctttcg
    14461 ctgattgttc ccttaagtct ttatacatcc caccttgtat tataccaaat aaagcttgct
    14521 tgtcggtatt attatgggca tctttacatc tttttaacca tcttgttgtt ctttctaaag
    14581 aattttttac atattctcta tctgatgggt atggtgcaca ctcatcaaat gccatcatta
    14641 tgtctgaacc caatgcattt tgtatttcca tagctttttc tggagttaga aaatgttttg
    14701 aaccatctaa gtgtgaccta aattctacac cttcttcttt tatctttctt aaaggcccta
    14761 gactaaatac ctggaaccct ccactatctg taagtattgg tttatcccaa ttcataaact
    14821 tatgaagacc accagctctt tttataagtt catgacctgg tctcatatat aaatggtatg
    14881 tgttactaag tattatttgt gaaccaatct cttttaattc ttctggtgtc atagatttta
    14941 cagttgcttg agttccaact ggcatgaata ttggtgtttc aataatccca tgtggagtgt
    15001 gtaatcttcc taaccttgca ccactctgct tacaagtctt tatcaattcg tatcttactg
    15061 cgtacataaa gttctcctta atcatattta tttctcatta tttcttaaga tatatttgta
    15121 ataagctatt catataattt attgtctaaa tagatttatc ttcgtcttct ttgttctgtt
    15181 gttccttaat tgcttcttca tcatgaatac tcattatatt aagagcactc ttgtcttttg
    15241 ttactaaaaa cttttttata gcaaatagtc ctgctatagc tataactcct attaaaacat
    15301 ctatcatacc ttcattttta ggcagtagca acatttttct agcaattgcg taaagtaatg
    15361 cctcaataaa tgctcctggt atatgaagag atagcattac tacaagttca acacctatta
    15421 ctaaaagtaa tatttgtgct aaaaaatcat taagctgact gtattgtact ggattttgaa
    15481 aatcaactat atatgctccc cacataagtc ttagaacatc tatagttcct aaaaatactg
    15541 ctattagaac tactactgct aaaacacgtt caaatataaa agcaatcttt aatatagggt
    15601 tattcttttt ttgtttttgt aaactatgca tccattacct ccctacttta taatcatagc
    15661 atctccaaaa ctgaagaatc tatatctttc ttttactgcc tcattatatg catttaaaac
    15721 attatccttt ccacaaatag cacttactaa cattattagt gtagactctg gtaagtgaaa
    15781 attagtaata agtttatcta aaactttaaa tttataacct ggatatataa atatttctgt
    15841 ccatccacta gtttctttca tctttccatc ctcattacaa gctgattcta tagttctaca
    15901 actggtagta ccaacacata ttacattttt tccattttcc tttgctctat ttattttgtc
    15961 agctgcttct tggctaacca tataatattc tgaatgcatc ttgtgattta aaacatcctc
    16021 tacttttact ggtctaaaag ttcctaagcc aacatgtaga gttacaaagg ctatatctac
    16081 acctttttct tttattttat ttaacaattc ttctgtaaaa tgtaatcctg ctgtaggtgc
    16141 tgctgctgaa ccattatgct ttgaataaac agtctggtat ctttcttttt catctagtct
    16201 agcagtgata tatggaggta gtggcatatt tccaagttca tccaatattt cttcaaaaat
    16261 tccatcataa tggaatttta taattcttga accttcatct gataaatcta caacttctcc
    16321 aattagcttt ccatttccaa aagaaaattt agtccctact tttgctcttt ttcctggttt
    16381 tactaaagcc tgccaagtat cttcctctgt tctttttaat aataaaaact ctattttacc
    16441 tccagtttca agcttctctc ctattaatct agctggtata actctagtat tatttaatac
    16501 taagcaatct cctggattta aatattctat tatatctcta aatactttat gttcaatatt
    16561 acctgtctct ttatctaata ccatcaatct tgagctagct ctatcttcta taggaacttg
    16621 agctataagt tcctgtggta agtcaaattt aaaatcactt gttttcaatg ttaaacttcc
    16681 tctcttaaaa tttctacagc tacaataata tcaaaaaaac aacttatttt ccacaatatt
    16741 accataatct atcaagaaat tgctattttt aacagacacc atatccacaa tatatagata
    16801 tttttccacg atatacatgc cttatccaca atatatagat acaatacaca caatttttat
    16861 cgtgtttatc cacagtatct acagatatag ttaccatttt gtggatatgt ttgaattttt
    16921 agccatttta gaaatatttt aatttaatga ctcttttatt ttataaatac tcgtataatc
    16981 tatttttatt tttcattagg atatggaatt tttaagtgtt catatgctaa tggcatggct
    17041 actcttcctc taggacctct atttataaat cctaattgaa gcaaatatgg ctcatataca
    17101 tcttctattg tatttctatc ttctccgata gaagctgcca atgtatctaa acccactggt
    17161 cctcctctaa atttttctat tatagtcata agaagcttct catctacaaa atcgagaccc
    17221 aaactatcga ctcctaaaag ttccagagca tcttttgcca ccttatcagt tatttttcca
    17281 tcagctctaa cttgtgcaaa atctctaact ctttttagta atctatttgc tatccttgga
    17341 gtccctcttg aacgttttgc aagttctaat gctccattag actgtatctc tgcatctaag
    17401 atgcttgaag acctaagtac tatttttgaa agttcatcaa ctgtataata gtccaattta
    17461 caaataacac caaatctatc tctaagtgga tttgtaagca taccagctct tgtagtagct
    17521 ccaataagtg taaatttagg aaggtcaagt cttatgcttc tagcagatgg ccctttccct
    17581 attattatat ctaggcaaaa atcctccatt gctggatata aaacttcttc aacacttcta
    17641 ttgattctat gaatctcatc tataaataaa acatcatttt catttaaatt cgtaagtatg
    17701 gctgccaaat ctccagctct ttctattgca ggtcctgacg taattcttaa atttactccc
    17761 atctcatttg ctattatact tgctagtgta gtttttccaa gacctggtgg tccatataaa
    17821 agcacatggt ctaactgctc atttctcgat ttagctgctt ctataaatat actaagttgt
    17881 tctttggatt tttcttgacc caaataatct tctaaagttt ttggtcttaa actgttttct
    17941 atatctatat cttccatctt cattgtagaa gttattatcc tattttcatc ttcaaaacct
    18001 tgcatttatg cccctcctct cgtacttttg aaaatctact ttatatattt tcaaatttac
    18061 ataaactcct taaatattgt tttaaaatcc aaatattata aaatccaaat actatttcaa
    18121 ggatttactc attatatatg taagtgcttt ctttataata gcttctgtat tcataccatc
    18181 ttttttacat ttttgaactg cctcttttga ttccgacaaa gtatagccta aagctacaag
    18241 tgcatctaca gactcatctt gtgatatcaa ggtaggtttt tgtgataata acgttggttc
    18301 aaactctatg ttatttttgt ctaccttatc cttaagctct aagacgattc tttctgctgt
    18361 ctttttacca acaccaggag ctttggataa ttttccaatg tcttcactta aaatatatgc
    18421 tcctaactgt gctggtgatg caaatgatag tattgaaagc gccactttag gtcctatttt
    18481 agatacagat gttagtagtt caaacatctt aagttcttcc ctacttacaa aaccacaaag
    18541 actcatatca tcttctctta caattaattt agtatatatt tttgtggctt ctcccacttg
    18601 taaattcatt attgtattgg atgatacatt tattttatat cctatcccat tattttcaac
    18661 aactatactg tctatataaa tttcttctac tgtacctttt atataactat acatacttac
    18721 cacctatatt ctttagagtt ttttcaagtt tgtttgaatg agcatggcaa atagccacag
    18781 ctaaagcatc tgcaacatca tctggttttg gaactttttt tagacttaaa aatgaagtta
    18841 ccatctgttg aacttgtgcc ttatctgctc tcccatatcc aactacacct tgttttactt
    18901 gaagtggagt gtattcatat acaggcttcc cattatgagc acatgcaagc atagtaacac
    18961 ctctagcttg tgcaactgtt atagctgttt ttacattctt gttaaagaat aattcttcca
    19021 ttccaacttc atctatattg taattcttta ctattgtatc aattccttta tacacaagtt
    19081 ccaatcttct cgatatattc atatgggcag gtgttgtaac tgctccataa tcgattactt
    19141 taaacttgct atttttgtat tcaattatac catatccaac tatggctata cctgggtcaa
    19201 tccccaatat tatcattttt taaaattccc cctaaaaata aaagaaatac caaaatgtat
    19261 ttctcataaa aacttatgtt ctaaatcatt atatcataaa taatattaac aaatcataaa
    19321 atgctctatg taaaatataa aatcaaataa aaaagttata tcaaagcaag attttacctc
    19381 aatataactt ttatatttat tacaaataaa
//
LOCUS       DBGJFF010000068        19311 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.069, whole genome shotgun sequence.
ACCESSION   DBGJFF010000068 DBGJFF010000000
VERSION     DBGJFF010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19311
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.069"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            236..757
                     /locus_tag="ACOIAF_002399"
     CDS             236..757
                     /locus_tag="ACOIAF_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGZ2669407.1"
                     /translation="MDIAKLILNPARLRILQYIRLHSSVRTSDIVKYLNDIPRATVYH
                     HVKILEENNMIEVIEENRVRGTIEKVYALKEYTTSMEGETFVALSTAFYVGLMQEMNE
                     YFSRKEQDHKQDNVFFSSALLYMSDNEYENLLKSIADLLKPYIEQKPKSNLKLRKLSI
                     ISSPPVADNDKTK"
     gene            791..1567
                     /locus_tag="ACOIAF_002400"
     CDS             791..1567
                     /locus_tag="ACOIAF_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HGZ2669408.1"
                     /translation="MKFHEFGNKNRPHIMLIHGGGNAWWNYLRQAEVLSNKYHVILPT
                     LDGHGEEYETTYISTEDTADKLISYIDENCSGHLFALCGVSLGGQIVMELLSRKPNIT
                     KKAIIDGSICYPRPLMARFCIAGIRFFGGLLFSEKACRFQIAMMPKLLPENMQYPDEI
                     KAYYMTDMPHVRKETFYNMYRTYMMNYTLKENVKKTTAQVMYWYGEKEMKCVKKSAKM
                     FQSYVPSCEIYEAKSYNHGYLSLYLPNEWLEIAEPFFQKE"
     gene            1909..2862
                     /gene="gpr"
                     /locus_tag="ACOIAF_002401"
     CDS             1909..2862
                     /gene="gpr"
                     /locus_tag="ACOIAF_002401"
                     /EC_number="3.4.24.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPR endopeptidase"
                     /protein_id="HGZ2669409.1"
                     /translation="MISVRTDLALEASEMCEKSQEGSSIPGVKIETKELENCIVTKVE
                     VIDRQGAEIMNKDIGKYITLESNLMKFDDDESREEMISYLKEELVDIFGQDKNKKTLV
                     IGLGNRNITSDALGPKSVSKTLVTRHLFKNYNKDYDDDFTEVSALSPGVMGVTGIETS
                     EIVKSLVEKVKPDRVVAIDALASRKMERVNSTIQISTAGISPGGGVGNTRKSLTKETL
                     GVDVIAIGVPTVVDAATLTIDVLDMAIDNLIAQSEETESFYEMLKKLKEEEKYHLIKD
                     SLDPYDKNLIVTPKDIDDTIENLSIIISEGLNRSLHPGRLV"
     gene            2880..3899
                     /gene="spoIIP"
                     /locus_tag="ACOIAF_002402"
     CDS             2880..3899
                     /gene="spoIIP"
                     /locus_tag="ACOIAF_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061783 - peptidoglycan muralytic activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HGZ2669410.1"
                     /translation="MFKKCIKVVTLTFILVCILPGKSLALNQDDFLKFLVNSSYPEAK
                     VEGNDTENKKNNKNKETSKENKEESKEENTKSKDASKVDNKKESEKEYIKLYVGKENV
                     PDIESKNSDTTETNTTSSSDYKDDLRVTKENPRILIYHTHGCETYSNSPDGNYHSRDK
                     KNSVMEVGSALTSALDSKGWGVVHTTKYHDYPSYNNSYASSLKTIQSILPKYNSVDIA
                     IDLHRDARDLTNPATKEKDHLKYTTMINGERVSKFFFVVGGKNTNRKQLRALAEDITA
                     FAEKKYPGLVSPIVEKDYARFNQFAVKNHMLVEIGNNATSVEESKATTKYLAEILDEY
                     FKQKN"
     gene            3930..4181
                     /locus_tag="ACOIAF_002403"
     CDS             3930..4181
                     /locus_tag="ACOIAF_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669411.1"
                     /translation="MSRLEKNIESKSKLRKYKYIMRILFLITMIIGTSVCVFIVDSNA
                     KSMLGMKQYDFPSIKFDITKFDFDIPNFDIHEIIKKFNK"
     gene            4463..6268
                     /gene="lepA"
                     /locus_tag="ACOIAF_002404"
     CDS             4463..6268
                     /gene="lepA"
                     /locus_tag="ACOIAF_002404"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HGZ2669412.1"
                     /translation="MDNKQSRTRNFSIIAHIDHGKSTLADRLIQQTGLVSERDMKSQL
                     LDNMDLERERGITIKLQNIRLMYKAKDGNEYYLNLIDTPGHVDFNYEVSRSLAACEGA
                     LLVVDAAQGVEAQTLANVYLAIDQDLEILPIINKIDLPSARPEEVKNEIEDLIGLDSS
                     EAPLISAKTGLNIEDVLEDIVKNVPPPKGDNEAPLKALIFDSYYDAYKGVVAYVRVFE
                     GTVKKGMTIKMMNTNKKFEVTEVGVMAPGQTELSELSAGDVGYIAASIKDIRSCRVGD
                     TITDSNNPTEEPLPGYKKATPMVYCGIYPGEGEKYENVRDALEKLQVNDAALEYEAET
                     SAALGFGFRCGFLGLLHMEIMQERLEREFNLDIITTAPSVIYRVTKMDGEVVMIQNPA
                     NLPEPSEIKMIEEPIVKGDIIVPKDYVGVVMELCQERRGNMLNMEYIDERRVMLHYDL
                     PLNEVVYDFFDALKSRTRGYGSLDYEVKGYVASTLVKLDILINKEQVDALSFIVHETR
                     AFPRGKAMCEKLKGEIPRHQFAIPIQAAVGNKVIARETISALRKDVLAKCYGGDISRK
                     KKLLEKQKEGKKRMRQIGSVEVPQKAFMSVLKLDE"
     gene            6417..7754
                     /locus_tag="ACOIAF_002405"
     CDS             6417..7754
                     /locus_tag="ACOIAF_002405"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HGZ2669413.1"
                     /translation="MINIEIPKKVDYIIKELEKNGYEAYIVGGCVRDCLLERVPNDWD
                     ITTSARPEVVVELFEKTIPTGIQHGTVTVMIEHEPFEVTTYRIDGNYSDGRHPDSIEF
                     TNNIVKDLSRRDFTINSIAYNSKTGLVDPFNGYEDIQNKYIRCVGNPVDRFEEDALRM
                     LRAVRFSAQLNFKIAEGTKQSIHKKADLIKNVSIERIQTEFNKILVSDSSKLNLLKST
                     GLLKFIIPEICELEDVTQHNPYHIYDVQKHTLIATEVIEDELYLKLTMLFHDLGKKVT
                     KTTDKNGVDHFYTHSRESVKIAKKILKRLKYDNYTINKVLILIQYHDYRIEPKRKIIK
                     KLLNKLEDVELFEDLIKVNWADTLAKNPKYAKQKILNLIECEKEFKHIINQKECFNLK
                     DLAINGKDLISIGIKPGKDIGYILNKMLEIVINNPELNEKEILKEKALNIYTF"
     gene            7987..9300
                     /locus_tag="ACOIAF_002406"
     CDS             7987..9300
                     /locus_tag="ACOIAF_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HGZ2669414.1"
                     /translation="MHTQLQKTIGLSAALSTVVGMVIGSGVFFKPQAIYTTTNGAPGL
                     GIIAWLLGGFITITAGLTATEISAAIPKTGGMMIYIEEIYGEKLGFLTGWMQTVLFFP
                     GTSAALGVIFAQQASELLGMSPNNMANVLPIAIGVILFLALLNIIGSSLGGKVQTVAT
                     IGKMIPLILIIVFGFIKGQSSEVLNPFVGDGVNASNALGQVLIATLFAYDGWINVGAI
                     SGEMKSPEKDLPRAIVGGLSLVMAVYIIINIAYLWVVPASELATVTSPATLVATRLFG
                     NIGGKVITVGILISVFGTLNGYLLTGSRIPYTLAEMGTLPASKTLLKVNSGGSPVNSI
                     LLITVLACVYALSGQFNLLTDLTIFSIWVFYVLTFIGVMRLRRQKPDLHRPYKVPLYP
                     IIPIIAILGGLFVIINQILTSTVISLGGIFITLLGLPVYYYMKKR"
     gene            9472..10647
                     /gene="hemW"
                     /locus_tag="ACOIAF_002407"
     CDS             9472..10647
                     /gene="hemW"
                     /locus_tag="ACOIAF_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HGZ2669415.1"
                     /translation="MLGLYVHIPFCVKKCKYCDFNSYKMDIDSKKRYIEDLKIEMELY
                     SNKLYKDNKYKNKECCSLNKNDKITSIFVGGGTPSILTSDEIREVFISIKEMFDIDEN
                     AEITIECNPGTLTLEKLKTMKEIGINRLSIGLQAIQEKHLNFIGRIHTYEEFEKNYKD
                     ALSVGFKNINIDLMYSLPNQTLCDWKETLEKVVHLNPTHISAYSLILEEGTELYNMYE
                     SNKFELIDENVDIEMYEYTINYLKSKGYNQYEISNYSKEGYNCEHNILYWECEHYIGI
                     GAGASGYINENRYNNVESLEDYHLSLVKREKPIQENEILSEKDMIEEKIFMGLRMNKG
                     IKFEDFKKKFGIDFREKYNKQIEMLLARKLINQSFEGIQLTQKGREISNSVFIEFME"
     gene            10750..11769
                     /gene="hrcA"
                     /locus_tag="ACOIAF_002408"
     CDS             10750..11769
                     /gene="hrcA"
                     /locus_tag="ACOIAF_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HGZ2669416.1"
                     /translation="MELNERKLNILKAIVKDYIETAEAIGSRTISKRHDLGVSAATIR
                     NEMADLEELGYLIQPHTSAGRVPSEKGYKLYVNSLMSKSELDDNDKILIEQCMNHNIN
                     HIKELIHETSKLLSQLTNYTTVAVTKSLINQSVIKHIQLVAMNDNNILLIVVTDKGDL
                     KKANLTTNVYLDQSKLNLISDNLTRKLLGKSITDLDDNLIAFIKYEISEYSGLIDELL
                     NALNSNMKEEDFSLSLNGATNIFSYPEFNDVLKAKSFLNMLEKKETIADIIKSKGIQK
                     DNLNIIIGSDNDCELAQDCSIVTATYNVDRDLVGRISFIGPTRMDYARIYSIINYMSL
                     LINRK"
     gene            11799..12419
                     /gene="grpE"
                     /locus_tag="ACOIAF_002409"
     CDS             11799..12419
                     /gene="grpE"
                     /locus_tag="ACOIAF_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HGZ2669417.1"
                     /translation="MDKKNEQQEVREENDTSINQESETQVELEEEVVNEECEKSSEKT
                     DEKEVDDENVTDINSKLAEKKLQDELDELNDKYQRLQAEYANYRRRTQQEKETIGVFA
                     NEKIITELIPVIDSMERALDACEDKEDTMYKGISLVHKQLIDTLVKFGVEEIEAESKE
                     FDPNLHLAVMQESVDGVEANQIVMVLQKGYKLGTKVVRPSMVKVSC"
     gene            12511..14358
                     /gene="dnaK"
                     /locus_tag="ACOIAF_002410"
     CDS             12511..14358
                     /gene="dnaK"
                     /locus_tag="ACOIAF_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HGZ2669418.1"
                     /translation="MGKIIGIDLGTTNSCVAVLEGGEAQIIANSEGMRTTPSVVAFTK
                     DGERIVGEPAKRQAVTNADKTITSIKTHMGTDYKVNIDGKSYTPQEISAIILQKLKSD
                     AESYLGQTVTEAVITVPAYFTDAQRQATKDAGRIAGLDVKRIINEPTAAALAYGMDKL
                     DQEKKILVFDLGGGTFDVSILEIGDGTFEVLATAGNNRLGGDDFDQIVIDYLAEEFKK
                     AEGVDLRNDKMALQRLKEAAEKAKKELSSTMSSNINLPFITATAEGPKHLNIDLSRAK
                     FEELTRGLVEKTMEPTKTALQDAGLSTGDIDDVLLVGGSTRIPAVQEAVKKFIGKEPH
                     KGINPDECVAAGASIQAGVLAGDVKDLLLLDVTPLSLGIETMGNVMTKIIERNTTIPT
                     KKSQIFSTAADNQTAVDIHVLQGERSMAYDNTTLGRFQLTDIPPAQRGIPQIEVTFDI
                     DANGIVNVSAKDLGTGKEQKITITSNTNLSEAEIEQKIKEAEMNAEADKQKKEKIEAF
                     NQAESTIYQTEKTLNELGDKISSGEKEDIEKAIADLKAVKDNQDATAEELKKATDEVM
                     TKFQKVSQEMYQKAAQEQQAAQGAEQAQDNGPKDDNVVDADFKEVDEDK"
     gene            14569..15723
                     /gene="dnaJ"
                     /locus_tag="ACOIAF_002411"
     CDS             14569..15723
                     /gene="dnaJ"
                     /locus_tag="ACOIAF_002411"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HGZ2669419.1"
                     /translation="MSTKRDYYEVLGISKGAEAQEIKKAYRKLAMKYHPDRNPGDKEA
                     EEKFKEINEAYEVLSDDTKRKTYDQFGHDGLNGQGGFGGQGGFGGQGFGGFEDMFGDI
                     FGDMFGGSFGGGRARRRGPQRGADIRQSVTISFEEAAFGKKMSIKVNRSEECEECNGT
                     GAKPGTSKKTCSTCNGTGQVRTVQRTPFGNIASSRPCSACNGTGEVIESPCSKCHGTG
                     NTRKVKTIEVDIPAGIDDGQMIKLSGQGEVGEKGAPRGDLYIVVNVKSHPLFTRDGND
                     IYFEMPITFVQATLGDEIEVPTLDGKVKYSVPEGTQTGTVFRLKEKGIPRIRGNSRGD
                     QYVKVVVEIPKKLNDKQKELLREFAKECGSNVHEKKKTFGQKIEDMFKKK"
     gene            16689..17624
                     /locus_tag="ACOIAF_002412"
     CDS             16689..17624
                     /locus_tag="ACOIAF_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HGZ2669420.1"
                     /translation="MYYIGVDIGGTGIQAGVVDNYGKIIFRAECKTVIEKGFEGILND
                     IKIMIYKLLEDNKLTMSDIKSIGFGVPGFINKEGLVTCVNLKWNKKAFNKELKRRFPD
                     VEIHGENDATVAALGEAKFGSMKGANVGVLYTLGTGVGGGIVINQKVFSGAHGLGSEI
                     GHQIIGENYFNCNCGNNGCVETFCSATAIIKYSQKLIEEGEKSRILDLAEGNLENVNA
                     KMVFDAYRENDLVAIKVINRFKEYLAKTFANTINSLDPEIISIGGGISKSSDIILDGI
                     EDLVRKFVLYKTEDIATITCATLGSDAGIIGAAFL"
     gene            17660..19033
                     /locus_tag="ACOIAF_002413"
     CDS             17660..19033
                     /locus_tag="ACOIAF_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGZ2669421.1"
                     /translation="MGKTNARRTLFLIAIGSGTMLNPLNSSMISLALHSIQNDFHISF
                     TTVSWIVSAFYLASAIAQPVSGKIGDLIGRKVLFLSGLMLVILSASIVPLVQSFFILI
                     FIRVIQAIGTSTLYPSGVALIQNNIKERQSSALAVLTIFASTTAGLGPTLGGLLLDLG
                     GWHAIFLVNIPVVLVSICLGYFLFPKEVKEKKSIKETLKNVLSRLDIIGILLFSIAMI
                     FILLFLLSMKESFNLEQLVFSIILMCLFIWHELRTKLPFIDIKLFVSNPKLSKVYLQF
                     IILNFFNYILFFGLPSYFQDALHYSAKSTGLFMLFMSGIGIFISPLTGKWIDKSGTRF
                     PLVTSSIFMFISAVLLSVVFIHIPVIGKGIILSLAGISYGVGNVALQSSMFEESPRDS
                     IGTASGLFQTSRYLGSILATVLLGMVFEVNITSEQFQILGYVMVVLGTISFLLNFILK
                     KELRKVE"
BASE COUNT         7666 a         2128 c         3519 g         5998 t
ORIGIN      
        1 taattaagat aatttttcca ataatcaaga taattttttt cattagtatg tatgatagat
       61 gcatcaaaca atataactaa tatgttagaa ggttatatgt aattaatgtc agtgtttata
      121 tagaatagca tttgtagcat ttattatagt tgtaattacc atttatatca tagacttgaa
      181 tagtctaaaa aaatagacta taatattttt atagactaaa gaaagggctg ataagatgga
      241 catagcaaaa ctaattttaa atcctgcaag gcttcgtatt ttacaatata ttcgacttca
      301 tagcagtgtt cgcacatctg atattgtaaa gtatttaaat gatattcctc gtgcaacagt
      361 ttatcaccat gtaaaaattt tagaggagaa caatatgatt gaagtaattg aagaaaatcg
      421 tgtgcgtggt actattgaaa aggtttatgc actaaaggaa tatacaacat ctatggaagg
      481 agagacattc gttgcacttt ctacagcatt ttatgtaggt ctgatgcagg aaatgaatga
      541 atactttagc agaaaagagc aagaccataa acaggataat gtatttttct cctcagcatt
      601 actatatatg agtgataatg aatatgaaaa tcttcttaaa agtatagctg atttattgaa
      661 accatatata gaacaaaagc ctaaatcaaa tctaaaactt agaaaacttt caattatatc
      721 ttctccacct gtagctgata atgataaaac aaaataacat gcaacccatt tttataaaag
      781 gaggttatat atgaaatttc atgagtttgg aaataaaaac agaccacata ttatgttgat
      841 tcatggggga gggaatgcat ggtggaacta tttgcgacaa gcagaagttt tgtctaacaa
      901 atatcatgtg atacttccta cattggatgg tcatggagaa gaatatgaaa caacatacat
      961 ttctacagag gatacagcag ataagttgat atcatatata gatgaaaatt gcagtggaca
     1021 tcttttcgct ttgtgtgggg tatcacttgg tggtcaaatt gtcatggaac ttttatctcg
     1081 aaaacctaat attactaaaa aagcaattat tgatggtagt atttgctacc caagacctct
     1141 tatggcaagg ttttgtattg ctggtattcg attttttgga ggacttttgt ttagtgagaa
     1201 ggcttgtcgc tttcaaattg ctatgatgcc aaaattgtta cctgaaaata tgcaatatcc
     1261 agatgaaata aaagcatatt atatgacaga tatgccacat gtaagaaaag aaacattcta
     1321 taatatgtat cgcacttaca tgatgaacta tacacttaaa gagaatgtta aaaaaaccac
     1381 agcacaagta atgtactggt atggagaaaa agaaatgaaa tgtgttaaaa aatctgcaaa
     1441 aatgtttcag tcttatgtgc cctcttgtga gatttatgaa gctaagagct ataatcatgg
     1501 gtatttatca ctttatcttc caaatgaatg gttggaaatt gcagaaccat ttttccaaaa
     1561 ggaataaagg tgttaggtga ttattgtata attattgctg ttttttaata tagtaaataa
     1621 atcaggtata aatataaaaa attattttag ttaatatatt agtgaaagct taataggaaa
     1681 attataaata gattaaaggt agcgcctata taaatagttg ctaccttttt ttgcttgtaa
     1741 ataaatgata gataagaaag tttactgaca aatcactgac taaacccaat tttcttacat
     1801 tgaaagcctt aattttactt gttttaaata aatttacaat ttcttgttat aaaatactaa
     1861 attttgttaa caattataat tagcatttaa tttatactgg aggtttatat gattagtgta
     1921 agaacagatt tagctttaga agctagtgaa atgtgtgaaa agtctcaaga aggaagtagt
     1981 attcctggtg taaaaataga aactaaagaa ttagaaaatt gcattgtaac taaagttgaa
     2041 gtaatagaca ggcaaggcgc agagataatg aataaagata taggaaagta tataactctg
     2101 gaaagtaatc ttatgaagtt tgatgatgat gagtcaagag aagaaatgat aagttattta
     2161 aaagaggagt tagtagatat atttggccaa gacaaaaata aaaaaaccct ggtaattggt
     2221 cttggaaata gaaatattac ttctgatgcg ttaggaccaa agagtgtatc aaaaacactt
     2281 gtaactagac atctttttaa aaattataat aaagactatg atgatgattt cactgaggta
     2341 tcagctttaa gcccaggagt tatgggtgtt actggaatag aaactagtga aatagtaaaa
     2401 tcattggttg agaaggttaa gccagataga gtagtagcaa tagatgcatt ggcttcaaga
     2461 aaaatggaga gagtaaactc tactatacag atatcaacag caggaatatc acctggtgga
     2521 ggtgttggca atactagaaa atctctaact aaagaaactt taggcgtgga tgttattgca
     2581 ataggcgttc caacagtggt tgatgcagca actcttacaa tagatgtctt agatatggca
     2641 atagacaatt taatagctca atcagaagaa acagaaagtt tttatgaaat gttgaaaaaa
     2701 cttaaagaag aagaaaaata tcatctaata aaagattcgt tagacccata tgacaagaac
     2761 ttaatagtaa caccaaagga tatagatgat acaatcgaaa atctatctat aataataagt
     2821 gaaggcttaa ataggtcttt acatccaggt agattggtat aaaataagga ggagcaatta
     2881 tgtttaaaaa atgcattaaa gtagtaacct tgacttttat attagtatgc attttaccag
     2941 gaaaatcatt agctttgaat caagatgatt ttttaaagtt tttagtaaat tcatcttatc
     3001 cagaagctaa agtcgaagga aatgatacag aaaataaaaa gaataataaa aataaagaaa
     3061 caagtaagga aaataaggaa gaaagtaaag aagaaaacac taaatctaaa gatgcaagta
     3121 aagttgataa taaaaaagag agtgaaaaag aatatataaa actttatgtt ggtaaagaaa
     3181 atgttccaga tattgagtca aaaaattcag acacaactga aactaacaca acatctagtt
     3241 cagattataa ggatgattta agagtaacca aagaaaatcc aagaattttg atatatcata
     3301 ctcatggatg tgagacttat tcaaattcac ctgatggaaa ttatcattca agagataaga
     3361 aaaattcagt aatggaagta ggaagtgcat taactagtgc tttagatagc aaaggatggg
     3421 gtgtagtcca tactactaaa taccatgact atccatctta taataactct tatgcaagta
     3481 gcttaaaaac aatacaaagt atattaccta aatataactc agtggatata gcgatagact
     3541 tacatagaga tgctagggat ttaactaatc cagccactaa ggaaaaagac catttaaaat
     3601 atacaactat gataaatgga gagagagtat caaagttctt ctttgttgta ggaggtaaaa
     3661 atacaaatag aaaacaactt agagctttag cagaagatat aactgctttt gctgagaaaa
     3721 agtatcctgg tctagtatct ccaatagtag aaaaagacta tgctagattc aatcagtttg
     3781 cagttaagaa tcatatgtta gtagaaatag ggaataacgc aacaagtgta gaagaatcta
     3841 aggctactac aaagtattta gccgagattt tggatgaata ttttaaacaa aaaaattaga
     3901 gctttaagtt ataattagga gcatgctaaa tgagccgatt agaaaaaaat atagaaagta
     3961 aaagtaaact aagaaaatac aagtatatta tgagaatctt atttttgatt actatgatta
     4021 taggtacatc agtttgtgta tttatagtag acagtaatgc aaaaagtatg ctaggaatga
     4081 aacaatatga ttttccaagt attaaatttg atataacaaa atttgatttt gacataccaa
     4141 attttgatat acatgaaatc ataaaaaaat ttaataagta gtaataagtg aataaggttt
     4201 attgattaaa ataagcctta ttttagttat atatttttaa tgtataaaat aaaaattcat
     4261 taactgtatt ttataggatt tactttaagt ttacgatatt atataatatt tagtgttaga
     4321 cttgataatc cacatataat gtggataatt ttgtttagtg ccaattagtt tatattcaaa
     4381 aattttaaat ttggtatata aagagttttt agataatgta aaataataac tataagaata
     4441 atcaagagga gggaatttta aggtggacaa taaacaaagt agaactagaa attttagtat
     4501 aattgcacat atagaccatg gaaagtctac cttagctgat agattaatac aacaaactgg
     4561 gcttgttagt gaaagagaca tgaaaagtca attacttgac aatatggacc ttgaaagaga
     4621 aagaggaatc actataaagc ttcaaaacat aagattaatg tataaagcga aggatggaaa
     4681 tgaatattat ttaaatctta tagatacacc tggacatgta gactttaact atgaggtatc
     4741 aagaagttta gctgcatgtg aaggagcact attagtagtt gatgcagctc aaggtgttga
     4801 ggcacaaact ttagccaatg tttatcttgc tatagaccaa gatttagaaa tattaccaat
     4861 aataaataaa atagatttac caagtgcaag accagaggaa gtaaaaaatg aaatagaaga
     4921 tttaataggg cttgattcaa gtgaagctcc tcttatttca gctaaaactg gtttaaacat
     4981 agaagatgtt ttagaagata ttgtaaaaaa tgttcctcca cctaaaggag ataatgaagc
     5041 tccattaaag gcattaatat tcgattctta ctatgatgct tacaaaggtg ttgtagctta
     5101 tgttagagta tttgaaggaa ctgttaaaaa gggtatgaca ataaaaatga tgaataccaa
     5161 taaaaagttt gaggtaactg aagttggtgt catggctcca ggtcaaactg aacttagtga
     5221 attatcagca ggtgatgttg gttatattgc tgctagtata aaagatataa gaagttgtcg
     5281 tgttggtgat acaataacag attcaaataa tcctacagaa gaacctttac caggatacaa
     5341 aaaagctact ccaatggtat attgtggtat atatccagga gaaggagaaa agtatgagaa
     5401 tgttagagat gctcttgaaa agcttcaagt aaatgatgct gcacttgaat atgaagctga
     5461 gacttcagca gcactaggat ttggttttag atgtggtttc ttaggtcttt tacatatgga
     5521 gataatgcaa gaaagacttg agagagaatt taaccttgat ataattacta cagctccatc
     5581 tgttatatat agagttacaa aaatggatgg agaggttgta atgatacaaa atcctgcaaa
     5641 cttaccagaa ccatctgaga taaagatgat agaagagcct atagtaaaag gagatataat
     5701 agttcctaaa gactatgtgg gtgttgttat ggagctttgc caggaaagac gtggaaatat
     5761 gttaaatatg gaatatatag atgagagaag agtaatgctt cactatgacc ttcctctcaa
     5821 tgaagttgtt tacgatttct ttgatgcttt aaaatcaaga actagaggtt atggttctct
     5881 tgattatgag gttaaaggtt atgtagcatc tacacttgta aaacttgata tattaatcaa
     5941 taaagagcaa gtggatgcac ttagttttat tgtccatgag acacgtgctt tccctagagg
     6001 aaaggcaatg tgtgagaaat taaaaggaga aattccaaga catcagtttg ctataccaat
     6061 acaagctgct gttggtaata aggttattgc aagagaaaca ataagcgctc taagaaaaga
     6121 tgttcttgct aaatgttatg gaggagatat atctcgtaag aagaaacttc ttgaaaaaca
     6181 aaaagaaggt aagaagcgta tgagacaaat tggttctgtt gaagttcctc aaaaagcatt
     6241 tatgtctgtg cttaaattag atgagtaaat ccttataaat ttagataaag atatttaaaa
     6301 gctagagtga ttttagaatt atttctaaat tagtctagct tttttatggt atattttaag
     6361 taaactcata gagaatataa gtaagaatat taaagaagaa taaaaaggtg atatttatga
     6421 ttaatataga aattcctaaa aaagtagact atataattaa agaattagaa aagaatggtt
     6481 atgaagctta tatagttgga ggatgtgtta gagattgttt attagaaaga gttccaaacg
     6541 attgggatat aacaactagt gctagaccag aagtggtagt agaattattt gaaaaaacaa
     6601 ttccaactgg tatacaacat gggactgtaa cagttatgat tgaacatgaa ccatttgaag
     6661 tgacaactta tagaattgat ggaaattata gtgatggtag acatccagat tcaatagaat
     6721 ttacaaataa catagttaag gacttgtcaa gaagagattt tactatcaat tcaattgcat
     6781 acaattcaaa gacaggatta gtcgacccat ttaatggcta tgaagatata caaaataagt
     6841 atatacgttg tgttggaaat ccagtagata gatttgaaga agatgcactt agaatgctta
     6901 gagcagttag attttcagca caactcaatt ttaaaatagc tgaaggtact aagcaaagta
     6961 ttcataaaaa agcagatttg ataaaaaatg tttctattga aagaatccag acagagttta
     7021 ataaaatatt agtatctgat tcatctaaat taaacttatt aaaatcaaca ggtttgttga
     7081 aatttataat tcctgaaata tgtgaattag aagatgttac tcaacacaat ccatatcaca
     7141 tctatgatgt acaaaaacat actttaatag ctacagaagt aattgaagat gaactgtatt
     7201 taaaacttac aatgttgttt catgatttag gtaaaaaagt aactaaaaca actgataaaa
     7261 atggtgttga ccatttttat actcatagta gagaatctgt aaagattgca aagaagattt
     7321 tgaaaagact taagtatgat aattatacta ttaataaagt tttaatatta atacaatatc
     7381 atgattatag aatagaacct aaaaggaaga taattaaaaa gttattaaat aaacttgaag
     7441 atgttgaatt atttgaagat ttaataaaag ttaattgggc tgatacactt gcaaaaaatc
     7501 caaagtatgc aaaacaaaag atattaaatc ttattgagtg tgaaaaagaa tttaaacata
     7561 taataaatca aaaggagtgc tttaatctta aagatttagc tattaatgga aaagatttga
     7621 tatctatagg tataaaacca ggtaaagata taggatatat tttaaataag atgcttgaaa
     7681 tagtcataaa taatccagag ttaaatgaaa aagaaatact aaaagaaaaa gcattaaata
     7741 tatatacatt ttagattcaa atataaaaaa tttaattact ttaaaaaaca tagaataaaa
     7801 tacttattta gataaaataa ataattttta gagaatattt tagaagttac gattgtatga
     7861 tttttaaatg cataggcatt tactctagga ctatagacta atataaaaaa aaactttggg
     7921 aatactaaaa ccataaattt ataaaattaa tattttatat actgtaaaac agtgaggagg
     7981 gacagtatgc atactcaact acaaaagact attggtcttt cagcagctct ttctactgtt
     8041 gtaggtatgg ttataggttc gggagttttt tttaagccac aagcaatata cactacaact
     8101 aatggagctc caggtctagg tattatagcg tggcttttag gtggttttat aacaataact
     8161 gctgggctta cagctacaga aatttcagct gctataccaa aaactggtgg tatgatgatt
     8221 tatatcgaag aaatatatgg agaaaagtta ggttttttaa ctggatggat gcaaactgta
     8281 ttatttttcc caggtacatc agcggcatta ggagtaatat ttgctcaaca agcatctgaa
     8341 cttttaggaa tgagcccaaa taatatggca aatgtacttc ctatagcaat tggagttata
     8401 ctatttttag cgttattaaa tataataggt tcatccttag gaggaaaagt tcagacagta
     8461 gccactattg gaaagatgat acctttaatt ttaataatag tctttggatt tattaaaggt
     8521 caaagtagtg aagtattaaa tccatttgta ggagatggtg taaatgcatc aaatgcactt
     8581 ggacaagtat taatagctac tttgtttgca tatgatggtt ggataaatgt tggagctata
     8641 tcaggagaaa tgaagtctcc agagaaagat ttaccaagag caatagttgg tggtttatca
     8701 ttagttatgg cagtttatat tataataaat atagcttatc tttgggtagt accagcaagt
     8761 gaattagcaa cagttacttc cccagctaca ttagtagcaa caagattgtt tggaaatata
     8821 ggtggaaaag taataactgt gggtatatta atatcagtat ttgggacatt gaatggatat
     8881 ttattaacag gttcaaggat accttataca ttagctgaaa tgggaacttt accagcatct
     8941 aaaactcttt taaaagtaaa ttctggaggt tctcctgtaa attcaatact acttataact
     9001 gtgttagcct gtgtatatgc attatctggt caatttaatc ttttgacaga cttaacaata
     9061 ttttcaatat gggtatttta tgtattgact tttataggtg ttatgaggct tagaagacaa
     9121 aagccagatt tacacagacc atataaagtc cctttatatc caataatacc aattatagct
     9181 atattaggtg gactgtttgt aataataaat caaatattga cttcaacagt catatcttta
     9241 ggcggaatat ttataacact attaggttta ccagtttatt attatatgaa aaaaagataa
     9301 ctacttaatt taagttagat aaatttgggt aaaggtgaaa attaactttt atccaaattt
     9361 tttatactgt tatacaatat aagtattata tattgcttga tgtgctattt tgtttgattt
     9421 ataatttagt aattgatgtt ttaagataat ttaagaaatg aggagtgaaa gatgctagga
     9481 ctttatgtac atattccatt ttgtgtgaaa aaatgtaagt attgtgattt taattcttat
     9541 aaaatggaca tagattcaaa aaaaagatat atagaagact taaaaataga aatggaatta
     9601 tatagtaata aattatataa agataataaa tataaaaata aagaatgctg tagtctaaat
     9661 aaaaatgaca aaataacgag tatatttgta gggggaggta cacctagtat actaacctct
     9721 gatgaaataa gagaagtatt tataagtata aaagaaatgt ttgatataga tgagaatgca
     9781 gaaataacta tagagtgtaa tcctgggaca ttaacattag aaaaattaaa aactatgaaa
     9841 gagattggca taaatagact tagtataggg ctacaagcta ttcaagaaaa gcatttgaac
     9901 tttataggaa gaatacatac ttatgaagaa tttgagaaaa actataaaga tgcattaagt
     9961 gtaggtttta aaaatattaa tatagattta atgtacagtc ttccaaatca aactctatgt
    10021 gattggaagg aaactcttga aaaagttgta cacttaaatc caacacatat atcagcttat
    10081 tcccttatac tagaagaagg aactgaacta tacaatatgt atgaaagtaa taaatttgaa
    10141 cttatagatg aaaatgtaga tatagaaatg tatgagtata caataaacta cttaaagtca
    10201 aaaggatata atcaatatga aatatccaac tactcaaaag aaggctataa ttgtgagcat
    10261 aatattttat attgggaatg tgaacattat atagggattg gagcaggagc ttctggctat
    10321 attaatgaga acagatacaa taatgtagaa tctttagaag attatcattt aagtttggta
    10381 aaaagagaaa aaccaataca agaaaatgaa attctttctg agaaagatat gatagaagaa
    10441 aagatattta tggggcttag aatgaataaa ggcataaaat ttgaagattt taaaaaaaaa
    10501 tttggaatag attttagaga gaagtataat aagcaaatag aaatgctatt agctagaaag
    10561 cttataaatc agagctttga aggtatacaa ttaactcaaa aaggaagaga aatatctaat
    10621 agtgtattta tagaatttat ggaataattt ttaaggtgtt gacaaaataa atttataatg
    10681 gtatataata tatataatct ttttagcact cgaacttagt gagtgctaac agctaggagg
    10741 tataataata tggaattaaa tgaaagaaaa ttaaatatcc tcaaagctat agttaaagat
    10801 tatatagaaa cagctgaagc tataggttct agaacaatat ctaaaagaca tgatttaggt
    10861 gttagtgctg ctaccataag aaatgaaatg gctgaccttg aagaattagg gtatttaatt
    10921 cagcctcata cttctgcggg tagagttcca tctgaaaaag gctataagtt atatgtaaac
    10981 tctcttatga gtaaaagcga attggatgat aatgataaga ttcttattga acaatgtatg
    11041 aatcacaata taaatcatat aaaagaattg attcatgaaa cttctaagtt attatcgcaa
    11101 ctaacaaact atacaactgt agcagttact aagagtttaa taaatcaaag tgttataaag
    11161 cacatacagt tggttgctat gaatgacaat aatattttac ttatagttgt aacagataaa
    11221 ggggacttaa aaaaagctaa ccttacaact aatgtatatt tagatcaatc taagttaaat
    11281 ttgatttctg ataatcttac aaggaagctc ttaggtaaaa gtataacaga tttggatgat
    11341 aatctaattg cttttattaa atacgaaata agtgagtatt ctgggcttat agatgagctt
    11401 ttaaatgctt tgaactcaaa tatgaaagaa gaagattttt ctctatcatt aaatggagct
    11461 actaacatat ttagttatcc agaatttaat gatgtattaa aagcaaagtc atttttaaat
    11521 atgttggaaa aaaaggaaac aatagctgac ataattaaat caaagggaat acaaaaagac
    11581 aatctaaata taataattgg tagtgataat gattgtgaac tggcacagga ttgtagtata
    11641 gttacagcaa cttataatgt agatagagac ttggttgggc gaataagctt tataggccct
    11701 accagaatgg attatgctag aatttactct ataataaatt atatgagttt attaattaat
    11761 agaaagtaaa gaaattttaa attgcgaggt gtaaagtctt ggataaaaaa aatgagcaac
    11821 aggaagtaag agaagaaaat gatacttcta ttaaccaaga atcggaaact caagtagagt
    11881 tagaagagga agttgttaat gaagaatgtg aaaaatcaag tgaaaaaact gatgaaaaag
    11941 aagttgatga tgaaaatgta acagatataa attctaagtt agcagaaaaa aaattgcagg
    12001 atgagttgga tgaattaaat gataaatatc aaagacttca agccgaatat gcaaactaca
    12061 gaagaagaac tcaacaagag aaagagacaa taggcgtatt tgcaaatgaa aaaataataa
    12121 ctgaattgat accagttata gatagtatgg aaagagcctt agatgcatgt gaagataagg
    12181 aagatactat gtataagggt ataagccttg tacataaaca attaatagac actttagtaa
    12241 aatttggagt agaagaaata gaagcagaat ctaaggaatt tgaccctaat ctacatctag
    12301 cagttatgca agaaagtgta gatggagttg aagcaaatca aatagtaatg gtacttcaaa
    12361 aaggatataa gctaggaact aaagttgtaa gaccttcaat ggttaaagtt tcttgctaag
    12421 tataaattaa aggttaaatt atttaaccta atataaaata ttaaaataag taaaacttaa
    12481 ttatataatt actcgaggag gattttagtc atgggaaaaa taataggaat agatttagga
    12541 acaacaaatt catgtgttgc agtgttagaa ggtggagaag cacaaataat agcaaatagt
    12601 gaaggtatga gaactactcc atcagtagta gcatttacaa aagatggtga aagaatagtt
    12661 ggagaacctg caaaaagaca agcagttaca aatgcagata aaacaataac ttctataaaa
    12721 actcatatgg gaactgatta taaagtaaat atagatggaa aatcatatac tccacaagaa
    12781 atatcagcaa taatattaca aaaattaaaa tcagatgcag aaagttactt aggacaaaca
    12841 gtaacagaag cagttataac agtaccagct tactttacag atgctcaaag acaagcaact
    12901 aaagatgctg gtagaatagc agggcttgat gttaagagaa taataaatga gccgacagca
    12961 gcagctcttg cttatggtat ggataaatta gaccaagaaa agaaaatatt agtatttgac
    13021 ttaggtggag gaacttttga cgtatctata cttgaaatag gagatggaac ttttgaagtt
    13081 ttagctacag ctggtaacaa tagattaggt ggagatgact ttgaccaaat agtaatagat
    13141 tatttagcag aggaatttaa aaaagctgaa ggtgtagatt taagaaatga taaaatggct
    13201 cttcaaagat taaaagaagc agcagaaaag gctaagaaag aattatcatc aacaatgagt
    13261 tcaaacataa acttaccttt cataactgct acagcagaag gaccaaaaca cttaaatata
    13321 gatttatcaa gagctaaatt tgaagaatta actagaggtt tagttgaaaa aactatggaa
    13381 ccaactaaga cagctttaca agatgcagga ctttctacag gtgatataga tgatgtatta
    13441 ttagtaggtg gttctacaag aataccagca gtacaagaag ctgttaagaa gtttatagga
    13501 aaagaacctc ataaaggtat aaacccagat gagtgtgttg cagcaggtgc ttctatacaa
    13561 gctggagttt tagcagggga tgttaaagac ttattattac tagatgttac tccattatct
    13621 cttggtatag aaactatggg taatgtaatg actaagataa tagaaagaaa tactacaata
    13681 ccaactaaga agtctcaaat attctcaact gctgctgata accaaacagc tgttgatata
    13741 catgttcttc aaggtgaaag aagtatggct tatgacaata caactttagg aagattccaa
    13801 ttaacagata taccaccagc acaaagagga atacctcaaa tagaagttac ttttgatata
    13861 gatgcaaatg gtatagttaa tgtttcagct aaagatttag gtacaggaaa agagcaaaaa
    13921 ataactataa catcaaacac taatttatca gaagcagaaa tagagcaaaa gataaaagaa
    13981 gcagaaatga atgcagaagc tgacaaacaa aagaaagaaa aaatagaagc attcaatcaa
    14041 gctgaatcta ctatatatca aactgaaaaa actttaaatg aacttggaga taaaataagt
    14101 tcaggtgaaa aagaggatat agaaaaagct atagcagact taaaagctgt aaaagataat
    14161 caagatgcta cagcagaaga acttaagaaa gctacagatg aagtaatgac taagttccaa
    14221 aaagtatctc aagaaatgta ccaaaaagca gctcaagaac aacaagcggc acaaggtgca
    14281 gaacaagcac aagacaatgg acctaaagat gacaatgtag tagatgctga ctttaaagaa
    14341 gtagatgaag ataaataagc tatataatat gtaacactaa atgataattg gaacatatat
    14401 gatttgtgaa aatcatatag atgttaaaga tatatggaca atatacatca taaagaatat
    14461 aatatatcat agattatata acttaaagta gaatataaga attaatgata gcttgtagtt
    14521 tattattcta caagctatcg ttttgtaaaa tgaaggtggt gtcaattttt gagtacaaaa
    14581 agggattatt atgaagtctt aggtatcagt aaaggtgcag aagctcaaga gataaaaaaa
    14641 gcttatagaa aattagcaat gaagtaccat ccagatagaa atcctggaga taaagaggcg
    14701 gaagaaaaat ttaaagagat aaatgaagct tatgaagttc tatctgatga tacaaagaga
    14761 aaaacttatg accagtttgg acatgatggg ctaaatggtc aaggcggttt tggtggtcaa
    14821 ggaggatttg gcggtcaagg ctttggtggt tttgaagata tgttcggaga tatatttggt
    14881 gacatgtttg gaggaagctt cggaggtgga agagcaagaa gaagagggcc tcaacgtggt
    14941 gcagatataa gacagagtgt tactatatct tttgaagaag ctgcatttgg taaaaagatg
    15001 tctataaaag taaatagaag tgaagaatgt gaagaatgta atggaactgg agcaaaacca
    15061 ggaacatcaa agaaaacttg ttcaacttgt aatggaactg gtcaagttag aactgttcaa
    15121 agaactccat ttggtaatat agcaagttca agaccatgta gtgcttgtaa tggaactgga
    15181 gaagtgattg aatctccatg tagtaaatgt catggaactg gaaatacaag aaaagttaag
    15241 actatagaag tagatatacc tgctggtata gatgatggtc aaatgataaa actttctggt
    15301 caaggtgaag taggagaaaa aggtgctcca agaggagact tatatattgt agttaatgtt
    15361 aagtcacatc cactatttac tagagatgga aatgatattt attttgaaat gccaattaca
    15421 tttgttcaag caactttagg tgatgaaata gaagtaccta ctttggatgg aaaagttaaa
    15481 tatagtgtgc cagaggggac acaaactgga actgtattta gacttaaaga gaaaggaatc
    15541 cctagaatta gaggtaattc aagaggagac caatatgtta aggttgtagt tgaaattcct
    15601 aaaaaattaa acgataagca aaaagaacta ttgagagaat ttgcgaagga gtgtgggtcg
    15661 aacgtccacg aaaagaagaa aaccttcgga caaaagatag aagatatgtt taagaaaaag
    15721 tagttaaagt agccaatata taatcatatt tttgaaatct tagttattta taagattgtc
    15781 aaaggagata ttatgatttt tatattggct atttttgtat aaaaaataaa tgtgcataaa
    15841 ataaaactga atataaataa ttttctgaaa aatacttgtc ctaaaatata taatatgcta
    15901 taatataatc aaataaaaga aaggggtgat tccagtggga acggatgtgg cactgccaaa
    15961 tgatgaagaa aactttattg taatatacaa aataaaatta gatactttta gtgctagatt
    16021 aagtactgga aaaactcatg agctgggata attattgtaa ttacctagac tcaaactttt
    16081 agtagtaaaa tagtaatatt atatagacaa ggtatctaat ttttaaatct tttgtatgaa
    16141 ttaaaatttt tataaagggt atatctgatt agaatgtaat tagggaaacc tagttgtgaa
    16201 ttacttttag tatatacatg tgtaaagaaa tatgtatatg gtattggtta ttgtgctaga
    16261 tgtgtatgta aaggaatatg tgtctagttg ggtatatgta aagaaatata cttataatct
    16321 atgaaaaatt tttagtataa attgctaaaa cattgagatt gttttttata gaaagttagt
    16381 ttataccaac taaaaatgtg cttaagattt gtttgatgtt taattataaa taaggttgta
    16441 gataaaaaaa taagtaaaga caaaggttta aaatacttgc tttaaatata gtttgttttg
    16501 aacacttgcc aaattaaaat aattaataaa atagacatct tctcatagta aaatatactg
    16561 tgagaggatg tttttgcttt gttcataaac ttttatgaaa aatttgcata gtgtcacatt
    16621 ttaatatact atatatataa taaaatatag ataaaattaa atgattcaaa agagaaaggt
    16681 gaaaagagat gtattatata ggtgtagaca taggaggaac aggtatacaa gcaggagtag
    16741 ttgataacta tgggaaaata atttttaggg cagagtgtaa aacagtaatt gaaaaaggct
    16801 ttgaaggaat acttaatgac ataaaaataa tgatttataa gttgttggaa gataataaac
    16861 ttactatgag tgacataaaa tctattggat ttggtgttcc aggttttata aataaagaag
    16921 gattagtaac ttgtgtaaat ttaaaatgga ataaaaaagc ttttaataaa gagttaaaaa
    16981 gaagatttcc agatgttgaa attcatggag aaaatgatgc aacagtagca gcacttggag
    17041 aagcaaagtt tggaagtatg aaaggtgcta atgttggagt attatatact ttaggaactg
    17101 gtgtaggtgg aggaatagta attaatcaaa aagtcttttc tggagcacat ggattgggtt
    17161 cagaaatagg tcatcaaatt ataggagaaa actattttaa ttgtaattgt ggaaataatg
    17221 gttgtgtaga aactttttgt tcagcaactg caattataaa gtattctcaa aaactgatag
    17281 aagaaggaga aaagagtagg attttagatt tggcagaggg aaatttagaa aatgtaaatg
    17341 caaagatggt gtttgatgca tatagagaaa atgatttagt ggcaattaaa gtaataaata
    17401 gatttaaaga gtatttagca aaaacttttg caaacacaat aaattcttta gacccagaaa
    17461 ttatatctat aggaggaggc atttctaaat ctagcgatat aatacttgat ggaatagaag
    17521 atttggtaag aaaatttgta ttatacaaaa cagaagatat agctacaatt acatgtgcaa
    17581 ctttaggttc agacgcagga attataggag cagctttttt ataatgtaaa tgaactgctt
    17641 attggaggcg tgatgatata tgggtaaaac aaatgctaga agaacgcttt ttttgatagc
    17701 aataggttca ggaactatgc tgaatcctct taattcttct atgatttcac ttgctttaca
    17761 tagtatacaa aatgattttc atattagttt tacaactgta tcttggattg tttcagcatt
    17821 ttacctagct agtgctattg cacaaccagt ttcaggtaag ataggagatt taataggtag
    17881 aaaggtctta tttttgagtg gtttgatgct tgtaatttta tcagcatcca tagtaccact
    17941 tgttcaatca ttctttatat tgatttttat aagagtaatt caagcaattg gaacaagtac
    18001 cctatatcca tctggagttg ctcttataca aaataatata aaagaaagac aatcttctgc
    18061 attagcagtt ctaaccattt ttgcttcaac tacagcagga ttaggtccga cacttggagg
    18121 attattgttg gacttaggag gatggcatgc tatatttctt gtaaatatac ctgttgtttt
    18181 ggtttctata tgcttagggt attttttatt cccaaaagaa gtaaaagaaa agaaatctat
    18241 aaaagaaact ttaaaaaatg ttttgtcaag attagatatt attggcatac ttctattttc
    18301 aattgcaatg atttttatat tactattttt gctatctatg aaggagtctt ttaacttaga
    18361 acaacttgta tttagtataa tactgatgtg tctttttatt tggcatgaat tgagaacaaa
    18421 attgccattt atagatatta agctttttgt aagtaatcct aaattgtcaa aagtttactt
    18481 gcaatttatt atattaaatt tctttaatta tattttgttt tttggtcttc ctagttattt
    18541 tcaagatgcc ttacattata gtgcaaagtc gactggatta tttatgctat ttatgtctgg
    18601 tataggtata tttatatctc cattaactgg aaaatggata gataaatctg gaactagatt
    18661 tcctcttgtt acatcatcaa tctttatgtt tattagtgca gtacttcttt cagtagtttt
    18721 tatccatata cctgtaatag gaaagggtat tatattatca ttagcaggta ttagttatgg
    18781 agttggtaat gtagccctgc aatcctctat gtttgaagaa agtcctcgtg acagtatagg
    18841 gacagcttct ggtctgtttc aaactagtcg ttatttaggt tctattttag caactgtttt
    18901 acttggaatg gtatttgaag taaatataac atcagaacaa tttcagatat taggttatgt
    18961 tatggtggtc ttaggtacaa taagtttttt attaaatttt atattaaaga aagaattaag
    19021 aaaagtagaa tagtggattt atggatttaa taagtctaat aactgataag tttaaaaata
    19081 ttgtttgtaa aacataactg gtttgatatt tattaattct aagttttgaa gcatagagta
    19141 ctcgataagt atacaatatt ttgaattaaa attaagcaga aatttgaact agtctttcaa
    19201 atctctgctt aatttagtag ttaaattttt ttatagagaa aataagattt gacacttaga
    19261 taaaaaatca ttgcattttc aaaattcttt aaatcaaacc caagtatctc a
//
LOCUS       DBGJFF010000069        19140 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.070, whole genome shotgun sequence.
ACCESSION   DBGJFF010000069 DBGJFF010000000
VERSION     DBGJFF010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19140
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.070"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(<1..784)
                     /locus_tag="ACOIAF_002414"
     CDS             complement(<1..784)
                     /locus_tag="ACOIAF_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HGZ2669422.1"
                     /translation="MTIKEVEEKTGLTRSNIRFYENENLIKPLRNERNGYREYSDENV
                     EDIKKIAYLRTLGITIDDIRNIILQKVSLVEVVEKQEKVLETKISDLENAKTMCNKML
                     ESDELSYLNLKVEQYITELPEYWNKNKPVFKLDSVSFLYIWGGKITWGIITIACLLIA
                     ITVFPKLPPQIPIQWNNGVANYVDKKFIYAYPVICVVFRILLRPFIWRWLQIHAFYSD
                     TIADYLTNFMCFVALSIEIFSILFIYNIARHVTIIIFIDILVF"
     gene            complement(1112..1642)
                     /gene="bilS"
                     /locus_tag="ACOIAF_002415"
     CDS             complement(1112..1642)
                     /gene="bilS"
                     /locus_tag="ACOIAF_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein BilS"
                     /protein_id="HGZ2669423.1"
                     /translation="MKSLVLYSSLTGNTKKIAYTIYDEIQEEKDIKDVNELVDYSIDY
                     ENYDIVFLGYWVDKGICDKNSKQVLENIHNKKIALFGTMGASKKGSYGASIIEKIESI
                     IPKDNEILGSFICQGKIAEGLKAKYKEMLKLSPDNEHIRQQLNNHEESQSHPDEQEIY
                     EASMFAKNMMIKASIV"
     gene            complement(1730..2824)
                     /gene="bilR"
                     /locus_tag="ACOIAF_002416"
     CDS             complement(1730..2824)
                     /gene="bilR"
                     /locus_tag="ACOIAF_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bilirubin reductase"
                     /protein_id="HGZ2669424.1"
                     /translation="MYCNLLKPIKIGNLELKNRVSFAPTSMGLKLEEKIKKFSDIAKS
                     GVALITLGDVSIRPSFHKVAISLSDEDGVMKYKKIVDEIHNSGAKVSAQLFCSDYDVN
                     LIKDTMKMGITSHDEIKKIMNDGVKDYITNMTKEEIKNIINLFKVTALNAKKAGFDMI
                     QIHGDRLVGSFSSSIFNNRNDEYGGTCDNRSRFASEIISSIRDEVKDIPIDYKFAIRQ
                     ENPHYGNAGVLLSEVEYFVKKFESLGVNSFHVTLANHSKLEDTIPTNNHPYFKDEGCF
                     LYLADEVKKHTNLPVCGVGKLSSPDFIESIISNNRVDMVSMSRQLLADSNWLQKVKDG
                     RVDEIKKCCYCNKKCADALQTRSQFGCILD"
     gene            complement(2826..3272)
                     /gene="bilQ"
                     /locus_tag="ACOIAF_002417"
     CDS             complement(2826..3272)
                     /gene="bilQ"
                     /locus_tag="ACOIAF_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bilirubin utilization transcriptional regulator
                     BilQ"
                     /protein_id="HGZ2669425.1"
                     /translation="MDFKNLQYESLSYIICNLQKNFKLYCEKCLKPYKLTNGLYFYLI
                     YINKNRNCSLNDVSTEFEVDKAHTTRTISRLEQDGYIEKIQNPNDSRAFQLRVTDKGE
                     EVLGDIKNIFSKWDNHIKKEFSDTEYKELVKNLHVVKDIKTAVEEE"
     gene            3578..4768
                     /locus_tag="ACOIAF_002418"
     CDS             3578..4768
                     /locus_tag="ACOIAF_002418"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HGZ2669426.1"
                     /translation="MISNKMQTLVANSSVIRAMFEEGKKLSDIYGEENVFDFSIGNPS
                     VEPPETIKAVINDILNEESPNLVHGYMNNSGYEDVRDAIAEHINKKDGLNLTRENLIM
                     TCGAAGGLNIILKTLLNPGDEVIAFAPYFGEYKNYTENYDGKLIEVPTNIDTFEPDLD
                     ALKNAITPKTRALIINTPNNPTGVIYSEELLKNLGELLDSKQKEFNTSIYLISDEPYR
                     EIIYDGAKVPCVLKYYRNSFIGYSYSKSLSLPGERIGYIVANGQMDDFDDVMSSLNVA
                     NRILGFVNAPSLFQRVIARSLDAEVDVNIYKKNFDLLYNSLIDMGYSCVKPNGTFYLF
                     PKAPIEDDKKFCNDAKQFNLLLVPGSSFGCPGHFRVSYCVSYDKVKSSLPAFEKLAKL
                     YNLK"
     gene            4938..5900
                     /locus_tag="ACOIAF_002419"
     CDS             4938..5900
                     /locus_tag="ACOIAF_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HGZ2669427.1"
                     /translation="MKKGYVYIILTTLFFSSMEISLKTVTNDFNPLQITLSRFFVGGL
                     VLLPFAVKRLRALSLSITKSDLKYFAFLGFMCVVVSMSLYQLAVLNTKASVVAVLFSC
                     NPVFVMLLAYLILKEKIYRHNVISLILEVLGIIVIINPLHTKLTLSGIFLTLSSAIIF
                     SMYTVFGKRKTLKFGGIVVTCFSFIFGSLEMLILVLLTKINFIADILNKNKLHVFANI
                     PIFSGYTLHNMPIMIYVFVFVTGVGYALYFMAMEATSTSLTSLVFFFKPVISPILALL
                     ILKEIIPINMVVGILLIVIGSIISIIPTIITQMHNKHVEALYKE"
     gene            complement(6022..6684)
                     /gene="zmp1"
                     /locus_tag="ACOIAF_002420"
     CDS             complement(6022..6684)
                     /gene="zmp1"
                     /locus_tag="ACOIAF_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease Zmp1"
                     /protein_id="HGZ2669428.1"
                     /translation="MRPSKKLLIAIISIFLISSVPVSAHADSTTIQQNKDTLSQIVVF
                     PTGNYDKNEANAMVNRLANIDGKYLNALKQNNLKIKLLSGKLTDEKEYAYLKGVVPKG
                     WEGTGKTWDDVPGLGGSTVALRIGFSNKGKGHDSINLELHETAHAIDHIVLNDISKSA
                     QFKQIFAKEGRSLGNVNYLGVYPEEFFAESFAYYYLNQDTNSKLKSACPQTYSFLQNL
                     AK"
     regulatory      complement(6784..6869)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(7055..9973)
                     /locus_tag="ACOIAF_002421"
     CDS             complement(7055..9973)
                     /locus_tag="ACOIAF_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021362860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HGZ2669429.1"
                     /translation="MKKGNRKALLISLIMILSMVVSTIYPTVSYASELGENSQIQSGS
                     TNSSTGEEKESDNKKPEQTPEKDKATDNKKPEQTPEEEKPTDNKKPEQTPEEDKSTDN
                     KKPEQTPEGEKPIDNKKPEQIPEEDKSTDNKKSEQALEDEKPLDNKNTEKTPEEDNLL
                     EDENLLKVLEEELNEENEDYGFVVKINNNTIETESMKKISFNLTYTPTSKGIQAGDSI
                     TFKVPDVFNKVNLDYTSECFDKTESNGEYTLTFRELPNGQSVMQGKIGLEAYVKKVDE
                     DTNAKIHIETTGKIESGSGDIDVEIKPGDKTDVPDAKGTLKKLVEGKKSTTVFMPVKN
                     KDINYSIQVNEKQEELKDIILYDELPEGLTLINGSVSVVTSDGKEVSDFNIEQSKNSI
                     SVNFGNIDKSYTVKYKARISDKNAKHGNKYKNVARIESDGKKIQEDDATVSIFDRGDD
                     YLLTKGHSGATNITQVGQVINYQISINDDKSPISNVVITDNIPEGMRLTTSGEAGHDF
                     RVVEIPMNGSWTPWSKEKIANNISYKVEEKRNESGQVDKVITGFTINLSKEEVESKFF
                     IAYTLKVISIEDSYINRAVLDANNSEIDKNDEINFKKNSGLISAKKEVDKKVLNSSDN
                     QIVKYKINMSTYGVYDAGQVNLLDEVNSALEISNIKYSDNLELKKEAGDGKNTIRLVN
                     KYEFKQIKEGEPVQSWVTFDADFTNVKVGETIQNVAQINGSSPPGVETTKQGYAFEAK
                     KVDALDKNVLSGAKFNLEDAFGNIVVKDLVSDEDGIIQSSVKKPGTYYLVEIMAPSGY
                     EKLKDKIKVEIGNKDIGRIVDIGNIENIRQENPPVNPPAPPNTDEPIVNPPVPPSTDK
                     PRKPSSSSDTEDTIVINPPVPPSEDIINPPIPEVLNPPVPPSEEMIETPVKQIIPIPE
                     VVKPSVSEEKNNKVKDDTLVNPPVPPKTGDSTTIIGEILLVIGAIVGLIVLRRNKNTN
                     "
     regulatory      complement(10091..10176)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            complement(10303..11481)
                     /locus_tag="ACOIAF_002422"
     CDS             complement(10303..11481)
                     /locus_tag="ACOIAF_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HGZ2669430.1"
                     /translation="MFSKILVKTFIRDSENVQNTDVRNKYGYVAGVVGILSNLLLFVI
                     KVFIGMLTSSIAIMADAFNNLSDMASSAITMIGFKLASKPADKEHPFGHGRIEYLSAL
                     IVAFMVMLVGLQFVKSSIERIVNPIPVKFEVIPLILLIASIMIKIWLSRFNKFMGNKI
                     DSSALKAVSLDALGDVFTSSCVVISFIVARFTNFPIDGYVGIVVSLVILYAGFSLVKD
                     TINPLLGEAPDEEMVNSIIELLLSYKYIIGTHDLIIHNYGVGRCIASIHAEIPSNIDI
                     MEIHEIIDTAEREISEKLDIYLVIHMDPICLEDKEVMSAKKELEEILKKNSLVKSMHD
                     FRIVGKGSKKNLIFDIVVNPSEFSKNMSEDDLKEDITKLVKEINPEYNCVIVVDKDFI
                     "
     gene            11856..12041
                     /locus_tag="ACOIAF_002423"
     CDS             11856..12041
                     /locus_tag="ACOIAF_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HGZ2669431.1"
                     /translation="MFFISDIYTKSPIKFDTPLQKEAYKILQKLDIDFECVDTDEAIT
                     MEDCVQINKKLNMKMVI"
     gene            complement(12106..14880)
                     /locus_tag="ACOIAF_002424"
     CDS             complement(12106..14880)
                     /locus_tag="ACOIAF_002424"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005388 - P-type calcium transporter
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HGZ2669432.1"
                     /translation="MRYYNKPTKEVLKYLKTNPEIGLDDNEVEERKLRYGLNEFTIKE
                     GRTFWDELGESLTEPMILILIGAAVISSFVGELHDALGILGAIFIGISIGIITEGKSK
                     KAAHALSKLTENIEVKVLRNGKIIKISKNDLVPGDIVYIETGDMIPADGRLIQSINLK
                     LREDMLTGESDDVAKNADAVLDMEVVYSKTEIIEQDAIPAKQVNMVFGGTLVAYGRGI
                     MVVTHTGDKTEMGKIAQNLSNEDQQTPLQIKLGKLGAKIAGISGIIATLLCMFMIIQM
                     QRKGMLILDTSSVLSFLQSLEPAKNAYMVCIALIVATVPEGLPTMINITLAITMQKMA
                     KINALVTKKEACETIGSVSVICSDKTGTLTQNKMMVEVAYVDGKYISGGEYQSNSYFE
                     QNCIVNSTADIEKEDNSFKYIGSATECALLLYHNDKNYNEMRKQTYLISQIPFSSEEK
                     KMSTLIRQEDSDILLSKGAPEVLLKKCSYVQQGKNIVPITPKVEKSILDEIKKLQIKS
                     MRTLGFAYKKMSNSKTEVAMTSEGELNLIGNSRSYMKEDNLVFSGFVGIVDPLREGVK
                     DSIDKAFNAGVDVKMLTGDNINTATAIGNELGLLNDGKKAVEATYIDVLTDKELREEI
                     KGISIVARSKPDTKMRIVSALQKSGEVVAVTGDGINDAPALSQADVGIAMGISGTEVS
                     KNAADIILTDDSFSTIVEGIKWGRGIYENFQRFIQFQLTVNIVAFIIAIISQLTGKDM
                     PFTTIQLLWVNIIMDGPPALALGLEPVRDYVLKRKPINRHSGIIARSMFVNIIINAIL
                     IITIVFTQSAFNILGATSEEQGTVIFSLFAFSALFNALNCREFGLNSTIPNFFKNKLA
                     LQIIVVTGIIQIIFTQVFQSFFNSVSLDFDMWIKIILFASTILLSNEFVKLILRTMKN
                     SRTMNFNSKN"
     gene            complement(15052..16308)
                     /gene="glyA"
                     /locus_tag="ACOIAF_002425"
     CDS             complement(15052..16308)
                     /gene="glyA"
                     /locus_tag="ACOIAF_002425"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HGZ2669433.1"
                     /translation="MLKTTKISDPELYKIVADELVRQEHNIEMIASESTAPTEVLELS
                     GCVFTNKTEEGYPGARFQAGSEEADKLETLAIKRAKEVFGAEHVNVQPYSGSTANYCV
                     YSSILKTNDTVLSMRLDQGGHLTHGSAVNFLHDIYKYEFYGVDPNTGRIDYDALEAKA
                     KECRPKLIIAGASSYPRLIDYERISKVAKEVGAYFMVDMAHVAGLVAAKVIPSPVPYA
                     DFVSSSTTKTFCGPRSGIVLCKAEHAKKLDKGVFPGTLGSIHLNTVAAKAFSLLYLST
                     DKFKKIMEQVVVNAQTLASELISHGFSIVSGGTDNHIVMVDLRSKNLTGKQFEKALEY
                     VGITVNKNVIPDDPQSPFVTSGVRIGLTSISQRGLKEKEVIQIAGIMNKVAENIDNKE
                     VLDECKAEAQELISKFPLYPEGYFED"
     gene            complement(16380..17882)
                     /locus_tag="ACOIAF_002426"
     CDS             complement(16380..17882)
                     /locus_tag="ACOIAF_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HGZ2669434.1"
                     /translation="MRALNVVERAGNKLPDPVTIFLLLCIIVVILSAVISNLGVEEIH
                     PSTKEVVKVVNLLEKEQIQSYLGSIVTNFQSFAPLGLVLVTMLGAGVAEKSGFMEVLM
                     KKGISKVPQKLVTVAIVFAGMLSHTAADVGFIILPPLAALVFLGIGRHPLVGMFAAFA
                     GVAGGFAANVMLSTTDVLLAGFTIPAAQMMDPSYQGNPAMNLYFAFISAIVLTFAGAF
                     VTEKFIAPRFTKYSDSTNDKDIQELSELENKGIKYALLSLLVVVVVIVALCIGDNAFM
                     KDPETGSILSSNAPLMKGIVPIITIIFLTPGLVYGKVSKKIKSDKDLVSMMGSSMSDM
                     GGYIVLAFIASQFINLFNLSNLGTILSITGAKLLAESGIPSYGLIIGFILLSGFINLF
                     VGSASAKWAILAPIFVPMFMLLDFNPALTQIAYRIGDASTNPISPLFPYFPVILAFAR
                     RYDKDIGIGTVISNMIPYSVVFTLIEIIILLLFMGIGIPLGPGGGISYVL"
     gene            complement(17973..>19140)
                     /locus_tag="ACOIAF_002427"
     CDS             complement(17973..>19140)
                     /locus_tag="ACOIAF_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HGZ2669435.1"
                     /translation="EKYKEDILDFFTDIHMHPELSFKEFRTTKAIKDLLVSLDIEILD
                     LGMETGVVGLLKGKYDGPTVALRGDIDALPIYEEVDIKYKSRYDGIMHACGHDIHTSC
                     LVGCAYVLSHIRDSLHGNVKFIFQPAEEVNKGAKMLVERGVMENPKVDAIFGLHNHPD
                     IPCGKIGVKLGGLMAAVDTIKIEVNGFGGHGGIPNRTIDPIVASSAIIMGIQTIVSRN
                     ISPLESAVISIGTINGGTANNVISEKVDMTGTCRSFSNEVRKKISENLENIVCEIARG
                     YQATAKLDYLFDLPAVINSKEMYAIACKSVCDLYSEDAIVDPIPSTGGEDFSIFMEKA
                     PGFFYWLGVGNKEQDCIYQWHNPKFKADKNSILVGTNVLCQSVINYMDKLKNKI"
BASE COUNT         6045 a         3306 c         2258 g         7531 t
ORIGIN      
        1 taaatacaag tatatctata aaaataatta ttgtaacatg tctggcaata ttgtaaataa
       61 aaagtataga gaagatttct atagacaacg ctacaaaaca cataaaattc gttaaataat
      121 ctgcaatagt atcactataa aaagcgtgta tttgtaacca tctccatata aatggacgta
      181 aaaggattct gaatacaaca caaatgacag ggtaggcata aataaatttt ttatctacat
      241 aattagctac accattattc cattggatag gaatttgagg tggcaacttt ggaaatactg
      301 ttattgcaat caacaaacaa gcaatagtaa taattcccca ggtaattttc cctccccata
      361 tatataagaa acttacagag tcaagtttaa aaacaggttt gtttttattc cagtattctg
      421 gcaactcagt tatgtattgt tctactttca aattcaaata acttagctca tcagattcta
      481 acattttatt acacatagtt tttgcatttt ctaagtctga tattttagtt tcgagaactt
      541 tctcctgttt ctctacaact tcaactaaag atactttctg taatataata ttacggatgt
      601 catcaatagt aattcctaat gtacgtaaat atgcaatttt tttgatgtcc tctacatttt
      661 catctgaata ttctctatag ccattacgct cattacgaag gggtttaatt aaattctcgt
      721 tctcataaaa acgaatatta gagcgtgtca atcctgtttt ttcttcaact tctttaattg
      781 tcattttaaa cactacctct ctattctatt aatttataac tataatatag tcttaaatat
      841 tttcactcta taaaattgta tatttagtat atcttaaaat caaactaatt atcttttata
      901 agttttcata caataatatc tcctttcaat aatatatctt tttttatatt taaaattatc
      961 ttattttcta taaaataatg tataaacaag gtttacagtc aagtattttt attatttttt
     1021 tgatttcata aaaaaagcat agcaaattat ttgctatact tttttactaa attatttatt
     1081 ctatatctta tatgaactta ttgtttaact actacacaat actagcttta atcatcatat
     1141 tttttgcaaa catactggct tcatatattt cttgttcatc tggatgactt tgagattcct
     1201 catggttatt taattgttgg cgaatatgtt cgttgtcagg agaaagtttt aacatctctt
     1261 tatattttgc tttcaatcct tctgctatct taccttgaca tatgaatgaa cctaatattt
     1321 cattatcttt tgggataatt gattctatct tttctattat actagctcca taacttcctt
     1381 ttttacttgc tcccattgtt ccaaatagtg ctatcttttt attgtgaata ttttcaagca
     1441 cttgttttga atttttatca caaattccct tgtccaccca atagcctaag aatactatat
     1501 cataattttc atagtcaata ctataatcaa ctaattcatt tacatcttta atgtcttttt
     1561 cttcttgaat ttcatcatat atagtataag caatcttttt agtatttcca gttaaacttg
     1621 aatataaaac taaacttttc atgatgtctc ctcctcttta tctaaatact tatctaaatc
     1681 accaaaaata tattaacctt aacaaattat ttatttttat aaggatattc taatctagta
     1741 tacatccaaa ctgagagcgt gtttgtaaag catctgcaca cttcttatta caataacagc
     1801 actttttaat ctcatctact cttccatctt ttactttctg taaccaatta ctatctgcta
     1861 gtaattgtct tgacatagaa accatatcta ctctattatt tgaaataata ctttctataa
     1921 aatctgggct agacaatttt ccaaccccac atactggaag atttgtgtgt ttctttacct
     1981 catcagctaa atataaaaaa cagccttcat ctttaaagta aggatgatta ttggttggta
     2041 tagtatcttc taattttgaa tgatttgcaa gtgttacatg aaaactgttt acacctaggg
     2101 attcaaattt tttaacaaaa tactcaactt ccgaaagtaa aactcctgca tttccataat
     2161 gaggattttc ttgtcttatt gcaaatttat agtctattgg aatgtcctta acttcatctc
     2221 ggatactact aattatttcg cttgcaaatc tacttctatt atcacacgtt ccaccatatt
     2281 catcatttct attattaaat atacttgatg aaaaacttcc tacaagccta tcaccatgaa
     2341 tttgaatcat gtcaaaacct gctttttttg cattcaatgc tgttacttta aataaattta
     2401 taatattttt aatttcttct ttggtcatat ttgttatgta atcttttact ccgtcattca
     2461 ttattttttt tatctcatca tgtgaagtaa ttcccatctt catagtatct ttaattaaat
     2521 taacatcata atctgaacaa aatagttgtg ctgatacctt tgcaccagaa ttgtgtattt
     2581 catctacaat tttcttgtat ttcatgactc catcttcatc cgataggctt atagctacct
     2641 tatgaaaact aggtctaata gatacatctc ctagagtaat cagagctact cctgacttag
     2701 caatatcaga aaacttttta attttttctt ccaatttaag acccatgctt gtaggtgcaa
     2761 aagatactct attttttaat tccaaatttc caattttaat tggttttaat aaattacaat
     2821 acatcctatt cttcctccac agctgttttt atatccttta ccacatgtaa attctttacc
     2881 agttctttat attctgtatc cgagaactct ttttttatgt gattatccca ctttgaaaat
     2941 atgtttttta tatcccctaa tacttcctca cccttatctg ttactctcaa ctgaaaagct
     3001 ctactatcat tagggttttg gattttttct atgtatccat cttgttctaa cctagatata
     3061 gttcttgttg tatgagcttt atcaacttca aattctgtag aaacatcatt caaagaacaa
     3121 ttcctatttt tattaatata aattaaatag aaatatagac cattcgtaag tttatatggt
     3181 tttaaacatt tttcacaata aagtttaaag tttttttgca agttacaaat aatatatgac
     3241 aaactttcat actgtaaatt tttaaaatcc atcttaaatt tcccttctat aattataata
     3301 tgtttgacta tatcaactat attatattaa acaattaata tagtcaagaa tattttattc
     3361 tctaaatcta tgttttatta attctttaaa aaactattat ataaaataat catgataaaa
     3421 ttagaaattg gaatttttaa tatgcactgt acataaatat attttatata aaagtttata
     3481 ttttcaaaca agtaattttt cgaaaattgt ctaaattcag taaaaatatg gtaatatata
     3541 cataagcaat ataaatctat aaaaaggagt gttacatatg atatctaata aaatgcaaac
     3601 tttagttgca aatagttctg ttataagagc tatgtttgaa gaaggaaaga aactttctga
     3661 tatttatggt gaggaaaatg tttttgattt tagtatagga aatccaagtg ttgaacctcc
     3721 agagactatt aaagcagtta taaatgatat tttaaatgaa gaatctccaa atttagttca
     3781 tggatatatg aacaactcag gatatgagga tgtaagagat gctattgctg aacacataaa
     3841 taaaaaagat ggattaaatc ttacaagaga aaatttaatt atgacttgtg gtgctgctgg
     3901 tggtctaaat attattttaa aaactctttt aaatcctggt gatgaagtta ttgcttttgc
     3961 accgtatttt ggtgagtata aaaattatac agaaaactat gatggaaaat taatagaagt
     4021 tcctacaaat atagatactt ttgagccaga tttagatgct ctaaaaaatg ctattactcc
     4081 aaaaacaaga gctttaataa taaacactcc aaataatcct actggtgtca tatattctga
     4141 agaattatta aaaaatcttg gagaattatt ggattcaaaa caaaaagaat ttaatacaag
     4201 catataccta atttctgatg agccatatag agaaataatt tatgatggtg ccaaagttcc
     4261 ttgtgtacta aaatactata gaaattcatt tataggatac tcttatagta aatcattatc
     4321 tctacctggt gaacgtatag gatatattgt tgctaatggg caaatggatg attttgatga
     4381 tgttatgtct tcattaaatg ttgcaaatag aatacttgga tttgtaaatg ctccttctct
     4441 ttttcaaagg gttattgcaa gaagtttaga tgcagaagtt gatgtaaata tttataaaaa
     4501 gaattttgac cttctatata atagtttaat cgatatggga tattcttgtg ttaaacctaa
     4561 tggtacattc tatttgttcc caaaagctcc aattgaagat gataaaaaat tctgcaatga
     4621 tgcaaaacaa tttaatttat tattagtacc aggttcttct tttggttgtc caggtcattt
     4681 tagagtatca tattgtgtat cttatgataa agttaaaagt tctttaccag catttgaaaa
     4741 attagccaaa ttatataatt taaaataact aaatcaaagt aattcaaata attaactacc
     4801 ttagtataga atacttacat tctatattaa ggtagtttat aattttatgt actatatttt
     4861 atacatacca tattattcca atatgcttga atattcttat ctatagtact aatataaatt
     4921 acaaggaggt gtttgacttg aaaaaaggtt atgtttatat aatactgact accttatttt
     4981 ttagttcaat ggaaattagt ctaaaaactg tcactaatga ctttaatccc ttacaaataa
     5041 ctttatcaag attttttgtt ggcggtttag tcttactacc ttttgctgta aaaagattaa
     5101 gagctcttag tctttctatt acaaaatcag acttaaaata ctttgcattt ttaggtttta
     5161 tgtgtgtagt agtaagtatg tcattgtatc aacttgctgt tctaaacaca aaggcatctg
     5221 tagttgctgt tttgttcagt tgtaatccag tctttgtaat gcttttagct tacttaattt
     5281 tgaaggaaaa aatatatagg cacaatgtaa tttcacttat attagaagtt cttggaatta
     5341 tagttataat aaatccattg catacaaaac taactcttag tggaatattc ttaacattat
     5401 cttctgctat tattttttca atgtatactg tatttggtaa aagaaaaacc ttaaaatttg
     5461 gtggaatagt tgtaacctgc tttagcttta tatttggtag tttggaaatg ttgatattgg
     5521 ttttactcac taaaattaat tttattgcag atatattaaa taaaaataag cttcatgtat
     5581 ttgcaaatat ccctattttt agtggatata ccttacacaa tatgcccatt atgatttatg
     5641 tattcgtatt tgttacaggt gttggttatg cgctttactt tatggcaatg gaagctacat
     5701 ctactagtct tacatcttta gtattcttct ttaaaccagt aatatctcca atactagcat
     5761 tattaatact taaagaaatt atccctataa acatggttgt aggaatttta ttgatagtaa
     5821 ttggttcaat aatttctatt atacctacga taattactca aatgcataat aaacacgttg
     5881 aagccttgta taaggagtag taattgataa tctatcatag ttatacataa gacaaaattt
     5941 attagattta aacgcaatat aaaagagatg ccattataat agcatctctt tatttatatt
     6001 tattttaaat ttattaaaac tctatttagc taaattttgc aaaaagctat aagtttgtgg
     6061 acaagctgat tttaacttac tatttgtatc ttgatttaaa tagtaatatg caaatgattc
     6121 tgcaaaaaat tcttctggat atacacctaa gtagtttaca ttacctaaag aacgaccttc
     6181 tttagcaaat atttgtttga attgtgctga tttagatata tcatttaata ctatatggtc
     6241 tattgcatgt gctgtttcat gtaattctaa atttattgaa tcatgtcctt tacctttgtt
     6301 actaaatcct attctaagag ctactgtact tccacctaaa cctggaacat catcccaagt
     6361 ttttccagta ccttcccatc ccttaggaac tacacctttt aaataagcat attctttttc
     6421 atctgttaac tttccactta ataattttat ttttaagtta ttttgcttta atgcatttaa
     6481 atactttcca tctatattag ctaatctatt taccatagca ttagcttcgt ttttatcgta
     6541 atttccagtt ggaaaaacta ctatttgact aagtgtgtct ttattttgtt gtatagtagt
     6601 actatctgca tgtgcactta ctggaactga acttattaag aatattgata ttatagctat
     6661 taataatttt ttacttggtc tcatattatt ccctcctttg agttaggccg ggagtaataa
     6721 atttcaaaat aaaaaagata cccaagagta tctttgtata tacaaaaaga aatttttact
     6781 cttttcggca actggctggc atggtttact aaactttagc ccctatagct ttgcgtgact
     6841 aggtttccct agttttgcct catttaattt tatatctata ttatagctta tatcttttta
     6901 attgtctata tctctttgga acttttttct aaaaagttat tattttttta cataaatctt
     6961 caaaaaaaaa tatcttctaa caatattttc attattaaat attgttttag aagatatttt
     7021 ttaatataac ttatattttt tataatgtta gggtctaatt tgtattttta tttcttctta
     7081 atacgataag tcctacaata gctcctataa ctaaaagtat ttctccaata attgtagtac
     7141 tatcccctgt ttttggtggt actggtggat ttaccaaagt atcatcttta actttattat
     7201 ttttttcttc tgacactgat ggctttacta cttctggtat tggaataatc tgtttaacag
     7261 gagtttctat catctcttct gatggtggta ctggaggatt caatacctct ggtattggcg
     7321 gatttattat atcttctgat ggtggtactg gtggatttat aactatagta tcctctgtat
     7381 cagatgaact tgatggtttc cttggtttat ctgtgcttgg tggtactggt ggatttacta
     7441 ttggttcatc tgtatttggt ggtgctggtg gatttactgg tggattttct tgtcttatgt
     7501 tttctatatt tccaatatcc actattctac caatatcttt attaccaatt tcaaccttaa
     7561 ttttatcctt cagtttttca tatccacttg gagccattat ctccactaaa taatatgttc
     7621 ctggtttttt tacactagat tgaattatac catcttcatc actaactaaa tccttaacta
     7681 ctatatttcc aaatgcatct tctaaattga acttagcacc tgaaagtaca tttttatcta
     7741 aagcatccac ttttttagct tcaaaagcat aaccttgctt agtagtctct actcctggag
     7801 gacttgaacc attaatctga gccacatttt gtatagtctc cccaactttt acatttgtga
     7861 agtctgcatc aaatgtaacc caactttgca caggttcccc ttcttttatc tgtttaaatt
     7921 cgtatttatt tactaatctt attgtatttt ttccatctcc agcttctttt ttaagttcaa
     7981 gattatcaga atattttata ttagatattt ctagagctga gtttacttca tctaaaagat
     8041 ttacttgtcc tgcatcataa accccataag ttgacatatt gattttgtat ttaacaatct
     8101 ggttatcact actatttaaa acttttttat caacttcttt ttttgcgctt attaatccag
     8161 aatttttctt aaagtttatc tcatcatttt tatcaatttc actattattt gcatcaagaa
     8221 cagctctatt tatatagcta tcttcaatac taattacttt taatgtataa gctataaaaa
     8281 actttgattc cacttcttct ttactgagat taattgtaaa tccagttata actttatcta
     8341 cttgcccact ttcatttctt ttttcttcta ctttatatga aatattattg gcaatttttt
     8401 ctttactcca aggagtccag ctcccattca ttggaatttc tacaactcta aaatcatgcc
     8461 cagcttctcc tgaagtagtt aatctcatgc cttctggtat attatctgtt ataacaacat
     8521 tacttattgg tgatttatca tcatttattg aaatctgata gtttattact tgaccaactt
     8581 gagtaatatt tgttgctcct gaatgtcctt tagtaagaag ataatcatct cctctatcaa
     8641 atatacttac agtagcatca tcttcttgta tcttcttacc atcagattca atcctagcta
     8701 cattcttata tttattacca tgttttgcat ttttgtcact gattctagcc ttatacttaa
     8761 cagtatacga tttatctata tttccaaaat taactgaaat agaattttta ctctgttcaa
     8821 tattaaaatc actaacttct tttccatcac ttgttactac acttacagaa ccatttatca
     8881 atgtcaatcc ttcaggaagc tcatcataca aaataatatc ttttaactct tcctgctttt
     8941 catttacttg tatactgtaa tttatatctt tatttttaac tggcataaat acagtagttg
     9001 atttttttcc ttcaacaagc tttttaagtg ttcctttagc atctggaaca tccgttttat
     9061 ctccaggttt tatttctaca tctatatctc cacttccact ttctattttt ccagtagttt
     9121 caatatgtat ttttgcatta gtatcttcat caaccttttt aacatatgct tccaaaccta
     9181 ttttaccttg cattacactt tggccattag gtaattctct aaatgtaagt gtatactctc
     9241 cattactttc tgttttatca aagcattcac ttgtataatc taaattaact ttattgaata
     9301 catctggcac cttaaaagtt attgaatctc ctgcttgtat tccttttgat gttggagtat
     9361 aagttaaatt aaatgatatt tttttcattg attccgtttc tattgtattg ttattaattt
     9421 ttacaacaaa tccatagtct tcattttctt cattaagttc ttcttctaaa actttcaata
     9481 aattttcatc ttctaataga ttatcttcct ctggagtttt ttctgtattt ttattgtcta
     9541 gtggtttttc atcttctaaa gcttgttctg actttttatt atctgttgac ttatcttctt
     9601 ctggtatctg ctctggcttt ttattatcta ttggcttttc tccttctgga gtttgctctg
     9661 gctttttatt atctgttgac ttatcttctt ctggggtctg ttctggtttt ttattatctg
     9721 ttggtttttc ttcctctggg gtctgttctg gttttttatt atctgttgcc ttatctttct
     9781 ctggagtctg ttctggcttt ttattgtcgc tttccttctc ctctccagtt gatgaattag
     9841 ttgaaccact ttgaatctga ctattctctc ctaattctga agcataagat acagttggat
     9901 atattgtaga cactaccata cttaaaatca taattaaact aattaataat gcctttctat
     9961 ttcctttctt catgtaaatc tcaccccttt aggttaccta taatttataa tctaatcttc
    10021 cattaatatc gtatataaag taatttttac taaaaaatta tacaaaaaaa gctatgaaaa
    10081 taaataaatt tatattcata gccggttgca ccactgactc agtatatcca taaggtgccc
    10141 aataatagaa tatacttcaa catctctata atatttcata aaaacaatta aaattgcttt
    10201 atttatatta aaaatacaat tatatacttc tattatatta tgcctctata ctattgtcta
    10261 tcctttttac attttttacc ttatgatgag attaattaat aattaaataa aatccttatc
    10321 tacaacaatt acacaattat attcaggatt tatctcctta actaattttg ttatatcttc
    10381 ttttaagtca tcttctgaca tgtttttgga aaattcggaa ggattaacta ctatatcaaa
    10441 tattaaattc ttcttgcttc cttttccaac tattctaaaa tcatgcatag attttacaag
    10501 actatttttc ttaagtattt cttcaagttc ttttttggct gacataactt ctttatcttc
    10561 aagacatatt ggatccatgt gaattactag atatatatct aatttttcag atatttctct
    10621 ctctgcagta tctataattt catgtatttc cattatatct atattggatg gaatttctgc
    10681 atgtatagat gcaatacatc taccaactcc atagttatga ataattaaat catgtgtacc
    10741 tattatatat ttataagata acaatagctc tataatagaa tttaccattt cttcatccgg
    10801 tgcttctcct aaaagaggat ttatagtatc ttttacaaga gagaaaccag cataaagtat
    10861 cactaatgat actacgatac caacatatcc atctatggga aaattagtaa atctagctac
    10921 aataaatgat ataactacac aacttgaagt aaatacatct cctaaagcat caagtgaaac
    10981 tgcttttaag gctgaagaat caattttatt tcccataaat ttattaaaac gactcaacca
    11041 gattttaatc attatagatg caattaaaag tataagtggt attacttcaa attttacagg
    11101 aattggattt actatccttt ctatagacga ctttacaaat tgaagtccaa ccaacatcac
    11161 cataaatgct actattaatg ctgataaata ctctattctt ccatgaccaa aagggtgttc
    11221 tttatcagct ggcttacttg ctaatttaaa acctatcata gttattgctg aagatgccat
    11281 atcagaaaga ttattaaatg catcagccat tatagctata ctagatgtga gcattcctat
    11341 gaataccttt atcacaaata aaagtaaatt ggataatata cctactacac cagctacata
    11401 accatactta tttctaacat ctgtattttg aacgttttcg ctatctctga taaatgtttt
    11461 gactaagatt ttggaaaaca tgaaacttac ctccctgccc tttattttat taacaaattt
    11521 tataatctat atctttattt atgataacat aatttatata taaaattctt aaaaatcact
    11581 ctacttcagt aaaatattat tctatatatc tatttagata cgaattttat atttagtaaa
    11641 gtttttatgg cttagtgtct gtttaataga ttctatataa gtttttacaa agattattta
    11701 ttatgataat atcatttttc ccaagtatta cttagaatat gatttttttg ttttgtctgt
    11761 acatatttta aatttatact atatttatct atattttata accttatgat acactataac
    11821 tataagattt taaatggaaa ggaatgaaat aaatcatgtt ttttatcagt gatatttaca
    11881 caaaatcacc tattaaattt gatactcctt tgcaaaaaga ggcatacaaa atattacaaa
    11941 aattagatat tgactttgaa tgtgttgaca cagatgaagc aattacaatg gaagattgtg
    12001 ttcagattaa taaaaaatta aatatgaaaa tggtaatata aaacaacatt aaacaaacaa
    12061 aaaccgcaaa gttacaatat atagtacttt gcggtttcat aagaattaat ttttactatt
    12121 aaagttcata gttctactat ttttcattgt tctaagaatt agctttacaa attcatttga
    12181 caataggata gtggatgcaa atagtattat ctttatccac atatcaaagt ccaaagaaac
    12241 agaattaaag aaactctgga acacttgtgt aaatattatt tgaattatcc ctgtaactac
    12301 tattatttgc aatgccagtt tatttttaaa gaaatttggt attgtactat ttaaaccaaa
    12361 ctctctacaa tttaatgcat taaaaagggc actgaatgca aataaagaaa atataacagt
    12421 accttgttct tctgatgtag ctcccaatat attaaaagct gattgagtaa atacaattgt
    12481 aattataaga atagcattta taataatatt tacaaacata gacctagcta ttattccaga
    12541 atgtctattt attggttttc ttttcaatac ataatctcta acaggttcta atcctaatgc
    12601 tagggctgga ggaccatcca ttattatatt tacccataat aattgtattg tagtaaatgg
    12661 catatcctta ccagtaagtt gagatattat agcaattata aatgctacta tatttacagt
    12721 aagctgaaac tgtataaatc tttgaaagtt ctcatatata cctcttcccc attttattcc
    12781 ctctactata gtactaaagc tatcatcagt caatattata tctgctgcat ttttagatac
    12841 ttctgttcct gaaataccca ttgcaattcc aacatctgct tgactaagtg ctggggcatc
    12901 atttatacca tcacctgtta cagctacaac ttcgccactt ttttgaagtg ctgaaactat
    12961 tctcattttt gtatctggtt tacttcttgc aacaatagat attcctttga tttcttctct
    13021 caattcttta tctgtcaata catctatata tgttgcttca actgcctttt tcccatcatt
    13081 taataatcct aattcatttc caatagctgt tgctgtattt atattatctc ctgtaagcat
    13141 ttttacatct acgcctgcat taaatgcttt atcaatggaa tctttgaccc cttctctcaa
    13201 tgggtccact attcctacaa atccactaaa aactaaatta tcttccttca tataacttct
    13261 tgaatttcct attaaattca attcaccttc tgaagtcatt gcaacttctg ttttagaatt
    13321 actcattttt ttataagcaa atcctaatgt tctcatagat ttaatttgta acttcttaat
    13381 ctcatctaaa atactttttt ctacttttgg agttattgga acaatatttt ttccttgttg
    13441 tacataagaa cattttttta ataatacttc tggagcacct tttgaaagta atatatctga
    13501 atcctcttga cgaattaatg ttgacatctt cttttcttct gaactaaatg gtatttgtga
    13561 tatcaaatac gtttgttttc tcatttcatt atagttctta tcattatgat ataaaagtaa
    13621 tgcacactct gttgcgcttc ctatatattt aaatgaatta tcttcttttt ctatatctgc
    13681 agttgaattt actatacagt tttgttcaaa atagctatta ctttgatact ctcctccact
    13741 tatatattta ccatcaacat atgctacctc taccatcatt ttgttttgtg ttaaagtccc
    13801 tgttttatcg gaacatatta cagaaacaga acctatagtt tcacaggctt ctttctttgt
    13861 tactaatgca tttatctttg ccatcttttg cattgttata gctaaggtaa tatttatcat
    13921 agttggcaaa ccttctggaa cagttgcaac tattaatgca atacaaacca tatatgcatt
    13981 tttagcaggt tctaatgatt gtaagaaaga taatacactg cttgtatcta aaattaacat
    14041 accttttctc tgcatctgta taatcataaa catacacaat aaagtagcta ttatgcctga
    14101 tattcccgct attttagcac ccaacttccc taatttaatt tgaagtggag tttgctggtc
    14161 ttcatttgat aaattctgtg ctatcttacc catctctgtc ttatcaccag tatgagtgac
    14221 aaccattatg cccctaccat atgctacaag agtccctcca aataccatat tgacttgctt
    14281 tgcaggtatt gcatcctgtt cgattatttc tgttttagaa taaactactt ccatatctaa
    14341 tacagcatca gcattcttag ctacatcatc agattctcct gttagcatat cttctctaag
    14401 tttcaaattt atagactgta taagtcttcc atctgctggt atcatatccc cagtttctat
    14461 atatacaata tctcctggta ctaaatcatt ttttgatatt ttaattatct tcccatttct
    14521 aagcaccttt acttctatat tttctgttaa ttttgacagg gcatgagctg cttttttaga
    14581 ttttccttca gttattatgc ctatagaaat tccaataaaa atagccccta aaatacctaa
    14641 tgcgtcatgt aattctccca caaaagaact tattactgct gctcctatga gaattaatat
    14701 cataggttct gtcaaacttt ctcccaactc gtcccaaaat gttctacctt cttttatagt
    14761 aaattcattt aatccatatc taagcttcct ttcttctacc tcattatcat caagtccaat
    14821 ttctggattt gtttttaagt attttaatac ttctttggta ggtttattat aatacctcat
    14881 aactttatcc cccttgttaa ttatctataa ggaattatat gcttatgata gcttgtatat
    14941 tacttttcaa aaaaaataag gtgttgatta atcaacacct tatagtggtt atatattatt
    15001 aaattttatg ttacaggctt tataaattgt gagctataca aatcaaaata tttaatcttc
    15061 aaaataccct tctggatata atggaaactt agatataagt tcttgtgcct ctgctttaca
    15121 ttcatctaat acttctttat tatcaatatt ttctgctacc ttattcatta tccctgctat
    15181 ctgtataact tctttttctt tcagacctct ttgagatatt gatgtaagac ctatacgaac
    15241 accactagtt acaaatggtg attgtgggtc gtcaggtata acatttttat taactgttat
    15301 tcctacatat tcaagagctt tttcaaattg ttttcctgta agattcttac ttcttaagtc
    15361 aaccattact atatgattat cagtacctcc acttacaata ctaaaaccat gtgaaatcag
    15421 ttcagaagct agagtctgag catttactac aacttgctcc attatctttt taaatttatc
    15481 tgtacttaaa tataatagtg aaaaggcttt tgctgcaact gtattaagat gtatggaccc
    15541 taatgttcct ggaaaaacac ctttatcgag tttctttgca tgttctgctt tacaaagtac
    15601 aataccactt cttggtccac agaaagtctt tgtcgtagat gaagaaacaa aatctgcata
    15661 aggtacagga ctaggtatta ctttcgctgc gactaatcct gcaacatgtg ccatatctac
    15721 cataaagtaa gctcctactt cttttgcaac ctttgatatt ctttcataat ctattaatct
    15781 tggatatgaa cttgcccctg caattataag ttttggtcta cattcttttg cctttgcttc
    15841 aagagcatca taatctatac gtccagtatt tgggtctacc ccataaaatt catatttata
    15901 aatgtcatgt aaaaaattaa ctgcgctacc atgtgtcaag tgtccacctt ggtcaagacg
    15961 catacttaaa acagtgtcat ttgtttttaa tattgatgaa tatacacaat agttagctgt
    16021 agaacctgaa tacggttgta cgtttacatg ttctgctcca aatacttctt ttgcacgttt
    16081 tattgctagg gtttcaagtt tatctgcttc ttcagaacct gcttgaaaac gagcccctgg
    16141 atagccttct tctgttttat ttgtaaatac acatccactt aattcaagta cttctgttgg
    16201 tgctgtactt tcagaagcta tcatctcaat attgtgttct tgtctaacca actcatcagc
    16261 tacaatcttg tataactctg ggtctgaaat ttttgttgtt tttaacataa taatcctcct
    16321 cttaattaaa gaatatattt tattagttta atcttcattg ataaatttat tataacggtc
    16381 tataaaacgt aagatattcc tccaccaggt cctaatggta taccaatacc catgaatagt
    16441 aacaatatta ttatttctat taatgtaaat accactgagt atggaatcat attcgatatg
    16501 acagtaccta tacctatatc tttatcatat cttctggcaa aggccaatat aactggaaaa
    16561 tacggaaaaa gtggacttat tggatttgtt gaagcatctc caatacgata agctatttga
    16621 gttaatgctg gattaaagtc tagtagcata aacatgggaa caaatatagg tgctaaaatc
    16681 gcccattttg cagaagcact tcctacaaat aaattgataa atccagatag caatataaat
    16741 cctattatca atccatagct aggtatcccc gactctgcta gtaattttgc tcctgttatt
    16801 gataaaattg ttcctaagtt actaagatta aataaattaa taaactggga tgctataaat
    16861 gctaggacta tatatccacc catatcactc atactactac ccatcataga tactaaatct
    16921 ttgtcacttt ttatcttctt acttactttt ccatacacta atcctggagt taaaaatata
    16981 atagtgatta taggaactat acctttcatt aaaggtgcat tactagatag tatagaacca
    17041 gtctctggat ctttcataaa tgcattgtca cctatacata aggcaactat cactactact
    17101 acaactaaca atgataataa agcgtatttt atacccttat tttcaagttc actcaattcc
    17161 tgtatatcct tatcatttgt tgaatcacta tattttgtaa aacgaggagc tataaatttt
    17221 tcagttacaa atgctcctgc aaaagttaaa actatcgctg atataaaagc aaaataaaga
    17281 ttcattgctg gattgccttg gtaagatggg tccatcattt gagctgctgg tattgtaaat
    17341 ccagctaaca atacatctgt agtactaagc ataacatttg ctgcaaatcc ccctgcaaca
    17401 cctgcaaaag ctgcaaacat acctacaaga ggatgtcttc caattcctaa aaacacaagt
    17461 gctgcaagtg gtggtaatat tataaatcct acatctgctg ctgtatgtga taacatacct
    17521 gcaaatacga ttgctacagt tacaagtttt tgtggaactt tacttatacc ctttttcatt
    17581 aacacttcca taaatccaga tttctctgca acacctgctc caagcattgt aaccagtact
    17641 aatcctaaag gagcaaatga ctggaagttt gtaactatac tacctaaata tgattgaatc
    17701 tgctctttct ctagtagatt tactaccttt acaacttctt ttgtagaagg atgtatttct
    17761 tctactccta aattagatat aactgctgac aatattacaa caattataca tagtaataaa
    17821 aatatggtta ctgggtctgg aagtttattt cctgctctct ctacaacatt tagtgccctc
    17881 atcaataatc cactatcttt gtttttttcc tgtgaattaa ttttcctcaa actaataccc
    17941 cctcataaac tttatctcca gaatgctcta tcttaaattt tattttttaa tttatccata
    18001 taatttataa cagactgaca tagtacattt gtacctacca atatagaatt tttatcagct
    18061 ttaaatttag gattatgcca ctgatatata caatcttgtt ctttgttgcc tacccctaac
    18121 cagtaaaaaa atcctggggc tttttccatg aatatagaaa aatcttcccc tcctgtagaa
    18181 ggtattggat caactatagc atcttctgaa tacaaatcac acacagactt acaagctatt
    18241 gcgtacatct ctttactatt tataacagct ggtaaatcaa ataaataatc tagctttgca
    18301 gttgcttgat atccacgagc tatctcacaa actatatttt ctaaattttc tgatattttc
    18361 tttcgtactt cattactaaa gcttctacat gtgcctgtca tatctacctt ttctgaaatt
    18421 acattatttg cagtccctcc atttatagta cctattgata taacagctga ttctaatgga
    18481 cttatgtttc tgcttactat ggtttgaata cccattataa ttgcacttga tgctactata
    18541 gggtctattg ttctgtttgg tatacctccg tgacctccaa atccatttac ttctatctta
    18601 atagtgtcta ctgctgccat taacccacct aacttaacac ctatttttcc acaaggtata
    18661 tctggatgat tatgaagtcc aaatatagca tcaacttttg gattttccat tactcctcgt
    18721 tccactaaca tcttcgcacc tttattgact tcttcggctg gttgaaatat aaactttaca
    18781 tttccatgaa gactatctct aatatgagac aatacatatg cacatcctac aaggcaagac
    18841 gtgtgtatat catgcccaca tgcatgcatt ataccatcat atctggattt atacttaata
    18901 tcaacctctt catatattgg caacgcatca atatctcctc ttagtgctac tgtgggacca
    18961 tcatacttcc cttttaataa tccaacaacg cctgtttcca ttcctaaatc taatatttct
    19021 atgtctaaac ttactaataa atcttttata gcttttgtag tcctaaattc tttaaaactt
    19081 aattcggggt gcatatgaat atctgtaaaa aaatctaaaa tatcttcttt atacttttct
//
LOCUS       DBGJFF010000070        18839 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.071, whole genome shotgun sequence.
ACCESSION   DBGJFF010000070 DBGJFF010000000
VERSION     DBGJFF010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18839
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.071"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(<1..629)
                     /locus_tag="ACOIAF_002428"
     CDS             complement(<1..629)
                     /locus_tag="ACOIAF_002428"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669436.1"
                     /translation="MFEKKKKELFPWKISALILVGVFVLSSGVYIGTRLKHNGVQTGE
                     VVSTSANMNEKSKEAFGLNKNCEVWLQSEGENETNYKRTLTMAGIVPESLLDKTKSEI
                     VSYFKKEYPTKKIKSMDKNEIILVESKYSDNKDVQAMDVTIKGKYTIENDNGNIALYK
                     YDDSGSKTLVEKTKIRVDSLPKTVQDEIKKGVVMDTEEEAYSRLEDFAS"
     gene            832..1155
                     /locus_tag="ACOIAF_002429"
     CDS             832..1155
                     /locus_tag="ACOIAF_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HGZ2669437.1"
                     /translation="MERINCNVINCSHNNSHICYADKIFINGEKAHNDRHTCCSSFLD
                     ESIYSNLSSNVNNRNCEELVCTVKNCKYNKDGSLCTLDNILVAPETDRVNLYIETCCS
                     SFECK"
     gene            1423..2277
                     /locus_tag="ACOIAF_002430"
     CDS             1423..2277
                     /locus_tag="ACOIAF_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HGZ2669438.1"
                     /translation="MSTFYIQFLYFVIYSFIGWCCETTYCSVLQKQFVNRGFLNGPFC
                     PIYGFGALAIVATLTPFVHNIPLLFLFSIIITSIMEYCTSFILEKIFNMTWWDYSKHK
                     FNIHGRVCLENSLMFGVLSLIVMLIVHPIVVDFINSISKNVLFIFAISIEIYFVLDLV
                     ITVHTILQLNGKLKQINLIIKELKDKKEYYKLITQETIENKLESLVENRLDTLEDRFE
                     ILKEKENYRYAKNRIDELKNKLNTLLSNHKLLHRRIIKAFPNISSNKHTDILNKIKEN
                     IKSIKKDK"
     gene            complement(2463..3899)
                     /locus_tag="ACOIAF_002431"
     CDS             complement(2463..3899)
                     /locus_tag="ACOIAF_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HGZ2669439.1"
                     /translation="MREVKPISIDILNTFKQVDEDRLNKLLADELKHLDRKIVVLDDD
                     PTGVQTVHDISVYTDWDKDSMEQGFNEKNSMFFILTNSRGFTVAQTTKAHKEISKNIV
                     DVSKKVNKDFIIISRSDSTMRGHYPLETNLLKSEVERLSGKLFQGEIIMPFFKEGGRF
                     TIDNVHYVKEGEILVPAGMTEFAKDKSFGYKSSDIGEWCEEKTKGKYKSSDMIYISSK
                     ELRELNIEAITEKLKKAEGFNKIIVNATDYVDVKVFTIAFIRAVNSGKEFMFRSAAAI
                     TKVLGGVSDKELLTKDELVSKGNTNGGIILVGSHVNKTTQQLEELKNCKYPIEFIEFN
                     QHLVLQENGLKNEVKRVIKVVEEKISSGKTVAVYTRRERFDLDTNDKDKQLMISVEIS
                     DAVTSIIGMLNVRPNFIIAKGGITSSDVGTKALRVKKATVMGQIKPGIPVWMTGKESK
                     FPNMPYIIFPGNVGEISTLRESVEILMG"
     gene            complement(3970..5352)
                     /locus_tag="ACOIAF_002432"
     CDS             complement(3970..5352)
                     /locus_tag="ACOIAF_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate:H+ symporter"
                     /protein_id="HGZ2669440.1"
                     /translation="MITGYGLLIAFVISIGILLVSIIKFKVNPFLALLITSIITGFMV
                     RMPINEISTTISTGFGNTLGSIGIVIGLGIIFGNILSESRATESIAKGLLAKTGEKNS
                     ALAVTTAGFLISIPVFMDAAFVIMMPIIKYVSRVTKKSLMVFVCALGVGTIVGHALVI
                     PTPGPLAVAANVNANVGSFILYSIIVAFPAALIGGWIYGKRFEKYPAYAIDENDREKN
                     LEQGKDSIKIEDDDSSKVPGFGISMFSLLFPILLILISNVFSMFLEKGSTMSGVLAFI
                     GDKNIAILLGILVAIGFLKKYINKPMGDVVIEAADSAGLILLITGSGGAFGSVINASG
                     IGNFLVDTMSGLSISVVVLGFLLSALLRISQGSATVALVTTSSILGPTILATGMSPVL
                     VGLAICAGGVGFSLPNDSGFWVLSRFSGLSVKDTLNSWTIGGTIAGVVAFIMVLLLSV
                     INGIIPLPGL"
     gene            complement(5488..6396)
                     /gene="garR"
                     /locus_tag="ACOIAF_002433"
                     /pseudo
     CDS             complement(5488..6396)
                     /gene="garR"
                     /locus_tag="ACOIAF_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426591.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
     gene            6789..7460
                     /locus_tag="ACOIAF_002434"
     CDS             6789..7460
                     /locus_tag="ACOIAF_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGZ2669441.1"
                     /translation="MATFKRLQEQAYDYLKELILSGEMIENEIYSETKLASEIGISRT
                     PIRDALQRLSQDGFIDIIPSKGFRIHQITANEIVEIFQIRSAIEGFCTFLITSQYKEA
                     RAVETISKLKHLLDKQKDILLGDKNLNSFAEYDTLFHTTIVSYAQNTEFDKMFNNYMY
                     RIKKLALDSLSHEGRLETTLKEHTDIFNNIANGCIEDIYRTTLIHMETPKYINLEDFC
                     NNKLF"
     gene            7563..8111
                     /locus_tag="ACOIAF_002435"
     CDS             7563..8111
                     /locus_tag="ACOIAF_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669442.1"
                     /translation="MIFLNEDVVTNETIDKDYAIEEVRMACKHFGDLYFYFSKVLFEE
                     FGEDKTSEILRKVLFERSEERAIAMRERAHENGDELIADNIISTTDVPFLGWVPEFEE
                     LHCPYGASWLLRFEENPWFKKFASLYCDVTDTTVAEVFTGDTSHKITKNILWGDKSCE
                     RIYFHDDKVSEGKYTYGNKNCK"
     gene            8373..8681
                     /locus_tag="ACOIAF_002436"
     CDS             8373..8681
                     /locus_tag="ACOIAF_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669443.1"
                     /translation="MFNKIYEKKEFIVFQVKKGYVVYNTRKSFEEGHTHLKHFEAAKT
                     AIDLAINKKIPRSKDGYYLTSLIRISDDGYYIDKLSELLYVREQKGKKEKYCNSGYQM
                     "
     gene            complement(8843..10633)
                     /locus_tag="ACOIAF_002437"
     CDS             complement(8843..10633)
                     /locus_tag="ACOIAF_002437"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2669444.1"
                     /translation="MKKSKGFKKTIGRLLPFVKKYKFSFIIAIICIISAATMNALAPK
                     TEGLIITQLTKDVISIAKGVPGASVNFDYVTKILVILACIYFANAIFTYTSSFLLTNA
                     IQNTMRDLRNEVENKIRRLPISYFDSNSFGDVLSRISNDVDTISNALQQSFMQIVNSI
                     LVIILALSMMFTINIYMALIALFIIPISYFVSKFVVKKSQSRFSLQQNALGKLNGKVQ
                     EMYTGFNEIKLYGKEEDSIKEFKKVNQELCENGFKAQFISSMMNPMVSLVTYFGIAAV
                     AVVGSIYAVSGGITVGNLQAFVRYIWQINQPLSQMTQLSTVIQSSFAAIERVFEILDE
                     EEEIPDVENPVKIENVKGNVTFEHVNFGYGENSTLIEDLNAEVKSGQMVAIVGPTGAG
                     KTTLINLLMRFYDVKKGAIKIDGVDIRDMKRKDLRSMFGMVLQDTWLFNGTIFENIEY
                     GRFGATKEEIIQAAKVANVHHFITTLPDGYNMFLNEEASNISLGEKQLLTIARAFISD
                     PSILILDEATSSVDTRLELMLQKAMRNLMNGRTSFVIAHRLSTIRNADLILVMNNGSI
                     IEQGNHDELMEKGGFYEKLYNSQFADKESE"
     gene            complement(10633..12360)
                     /locus_tag="ACOIAF_002438"
     CDS             complement(10633..12360)
                     /locus_tag="ACOIAF_002438"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2669445.1"
                     /translation="MKLILSYLKNYKLLIVLNILAIFSFALVELGIPTIIAKIIDNGI
                     ANQNIAYIKQMGIVIVIISIIGVVGSILLGYCSAKVSTSVTRDIRNDIFEKSQEFSHT
                     EYNKFGISSMITRTTNDAFQIQQFVNILLRTALLTPVMFIISIIMTIRTSVELSIVLA
                     ISVPFIIIGVAIIAKVSQAISSKQQKGLDKLNLISRENLTGIRVIRAFGNDDYETERF
                     EKTNTYYANVSKKLFKLMSITQPAFFLLLNIAVLAVFWISSEKINIGELQVGQLVAFL
                     EYLFHAMFSIMLFSMVFIMYPRAEVSANRIKELLNEEPLIKNPENGIKDTENKGIIEF
                     DNVTFTYPDGEASVLKDISFTAKTGETVAFIGSTGSGKSTLINLIPRFYDVTEGSIKI
                     NGVDIREYDLKALRKKIGFIPQKSLLFTGSIANNIRFGKHKAGESELEYSAKVAQAYE
                     FISKKPRKFDELISEGGANVSGGQKQRLSIARAIIRRPEIYIFDDSFSALDFKTDAIL
                     RAKLKKETKDAIVLIVAQRISSIIDADKIIVLNEGQVVGMGTHKELLKNCEIYYEIAT
                     SQLKKEELE"
     gene            complement(12605..12871)
                     /locus_tag="ACOIAF_002439"
     CDS             complement(12605..12871)
                     /locus_tag="ACOIAF_002439"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HGZ2669446.1"
                     /translation="MNLEPKKLIEQIGKEKDKKDIWIVGGGDIVKLFMKDNLIDDYYI
                     YILPIILGRGISLFKSGIDKTNLNFKKASNIGELVKLEYSKKQK"
     gene            13169..14281
                     /locus_tag="ACOIAF_002440"
     CDS             13169..14281
                     /locus_tag="ACOIAF_002440"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HGZ2669447.1"
                     /translation="MKISEVATPNFLLDLDQLEKNIDKIQQICTNNNKQLWPMLKTHK
                     STYIARLQKNAGASGFLVGTLDEGEALIKAGFEDIMLAYPYMGEVNINRIVTMASKIN
                     LICATDNIECAKAYSEAFSKSKIECKLLIIVDCGLHRFGVEPEKVVELATEISKLENI
                     KIVGISSHPGQVYGCSSPEGVPDVCVQEDSSMEKAYKALTDNGFNIEIVASGSTPTVE
                     SEAASKTITAVRPGNYVYYDAIQIGLGCATEDMCALTVVCTVISKNSSGYYLIDCGSK
                     CLGLDKGAHGNSSIVGYGRVCGHPELTIDSLSEEVGKIKVNGETDIKIGDRIRIIPNH
                     SCSTANLTSNLLGFRGDTIEKVIEVDIRENSKQIIL"
     gene            complement(14646..15155)
                     /locus_tag="ACOIAF_002441"
     CDS             complement(14646..15155)
                     /locus_tag="ACOIAF_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HGZ2669448.1"
                     /translation="MSQQNTGNAIVNSKPKKTAVEIFMGGAKKGFYIGVEQILPAMIM
                     GYVIVQFLQLTGLVDILGNIFGPVMGIFGLPGESVVVLIAAFFAKAAGAATAANLFAQ
                     GLITAPQATILIMPCMLMGTLVGHYARIVLVANASTKYRMLLLAVPIFDAIVGMLIVR
                     VLLTVMGLM"
     gene            complement(15157..15804)
                     /locus_tag="ACOIAF_002442"
     CDS             complement(15157..15804)
                     /locus_tag="ACOIAF_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HGZ2669449.1"
                     /translation="MDVTAGDNKVTWKGYLSLFVLIVLFSGVFKDSQGFLKALDFANL
                     TGSFGTIADGVDFMGKGGTGAKDGFLFALTLIPTVCFAVGLIDVVESMGGMKAASKIF
                     NPLLRPLLGIPGIAGIAFVSSFTSSDVASIMTKELFESGEITDDERTIFVAYQYAGSA
                     VILNTINTQAPLLPIALLALGPIILIEIFCKILGANLVRLIIGVRNKKNKAKEAV"
     gene            complement(15818..16990)
                     /locus_tag="ACOIAF_002443"
     CDS             complement(15818..16990)
                     /locus_tag="ACOIAF_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HGZ2669450.1"
                     /translation="MNTYLNEISKFVDENREEIVSLWKEIVNMESYTHCKESVNKLAE
                     RLKLEFEKEGLDCELVDIGDNGSTLIGTLGSNIDKKPIIFSGHMDTVFETGTFGENPF
                     KIIEGKAYGPGVLDMKGGIIISLYAIKALNKIGYKERPIKIVFSGDEEIGHKDSTGAD
                     VILREAKGGLCAFNMETGLVDNSLCVGRKGRIGCNIHVKGVETHAGNDFEGGRNAIEE
                     MANKILRIQKLTNLDVGTTVSVSMIKGGRVENSIPEDCSIKVDLRFEKVEEMENVKEQ
                     VREICKETYIEGTSTEVNFISEMMPFETTEDVMRFHTFVNEVSKENGFGELNAKRLGG
                     SSDASYLTIANVPTICSFGVRGEWNHTSREYAVVDSMFERVKLISTVILNLDKFEV"
     gene            17258..18634
                     /locus_tag="ACOIAF_002444"
     CDS             17258..18634
                     /locus_tag="ACOIAF_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669451.1"
                     /translation="MKYKIGFLTTSYFMSDKFKQALKELNDTCEITFIELKGENPFNN
                     LPNIYYEQVDNFDGFVCSGIVPYSELIINIKDIKVPLNFLKLDERDFYKYLFKLLNSQ
                     KDIDFTKSFMDFLREDNNYYDIYSLIDSNKCPYTIKDFNIPQPVYDFKKLNDKLLEIH
                     LDLLRDNKINLSFTRNYIISCELNKLGYDCIYMVPSTESILNTFKSLVNEITLQNLDK
                     NKSATCIVTVNSSEYINEDLIFKNDEIQNQIYNTILNSLSRHGFYGVQVKKNDMKIEI
                     HTTKEMLNNMTNNYTDFFISEDLKEIKYSLNIGWGIGNTNIHAEQNARQANGKSASLN
                     GNCTFIVNDTNDTIGPLYSNKNSNNIEDSSIANKVASFIPLSNTNVSKIMCMINDRNS
                     NNVSAEILSDYMNITLRSANRILSILYKAGIATIVNTKLDNQRGRPKKIYKVDFLSFL
                     NKMQKLNS"
BASE COUNT         6522 a         3081 c         2260 g         6976 t
ORIGIN      
        1 taacttgcaa agtcttctaa tcttgaatag gcctcttctt cagtatccat aactacacct
       61 tttttgattt catcttgaac tgttttagga agtgaatcta ctcttatttt tgttttttct
      121 actaatgttt tacttccgct atcatcatat ttatatagtg ctatatttcc attatcattt
      181 tcaatagtat atttaccttt tatagtaaca tccattgctt gtacatcttt attatcacta
      241 tatttagatt ctaccaatat gatttcattt ttatccatac tctttatttt tttagttgga
      301 tattcttttt taaaatatga tactatttct gacttcgtct tatctaataa tgactctgga
      361 actattcctg ccattgtaag tgttctctta tagttagttt cattttcgcc ttcactttga
      421 agccaaactt cacaattttt atttaatcca aaagcttctt ttgacttttc attcatattc
      481 gcactagtac ttacaacttc accagtttga actccattgt gttttaatct tgtccctata
      541 taaactccgc tacttaatac aaatactcca accagtatta aggcagatat tttccaagga
      601 aataattctt ttttcttctt ttcaaacatt gattacgcct cctttttcta ttattttctc
      661 caagctgtaa gtttttatac aatttaggta aattttagaa gaaaatatat ttatatttca
      721 aattactcta agaatagata ttttctaaaa ggtagttttc tcttaatggt tatattcttg
      781 taattatatg gacataattt ataataaata atcgtttagg aggtaataac tttggaaaga
      841 ataaactgta atgtaattaa ctgttcacat aacaacagcc atatatgtta tgcagataaa
      901 atttttataa atggtgaaaa agctcacaat gacagacata cttgttgtag ttcattttta
      961 gatgaatcta tttatagtaa tttatctagc aacgttaata atagaaattg tgaggaatta
     1021 gtttgtactg taaaaaattg taaatacaac aaagatggta gcttatgtac acttgataat
     1081 atattggttg ctcctgaaac tgatagagta aatctttata ttgaaacatg ttgctctagt
     1141 ttcgaatgta aatagtcata gtataaatat aagggtgtca aatgtaatta aactaacata
     1201 gtgtagagta tctacaacta ttagtttaat tatacttgac acccttattt attttaaata
     1261 aaatttcatt attataacca tgtttataat gttctaactg taattaaatt tgataaattt
     1321 ctataaattg gtataatcaa tatgtaaatt gaatttagca aattaatcat aaaaataaaa
     1381 tatcgagtat atagtgttat actaactaga aatgaggtgc ttatgagtac attttatatt
     1441 caatttcttt attttgtaat ttatagcttt ataggatggt gttgtgaaac tacatattgt
     1501 tcagttttac aaaaacaatt tgtaaataga ggttttttaa atggtccatt ttgccctata
     1561 tatggatttg gagcattagc aatagttgct accttgactc cttttgtaca caatattcct
     1621 ttgttatttt tgtttagtat aattataact agcataatgg agtactgtac gagctttatt
     1681 ttagaaaaaa tatttaatat gacttggtgg gattattcta aacataaatt taatattcat
     1741 ggaagagttt gtctggaaaa ctcacttatg tttggtgtat tatcattgat tgtaatgctt
     1801 atagtacatc ctattgtggt tgactttatt aactctatct ctaaaaatgt gctatttata
     1861 tttgctatat ctatagaaat ttattttgtt ttagatttag ttatcacagt ccatacaatt
     1921 ttacaattaa atggtaagtt aaaacaaatc aatcttataa ttaaggaatt aaaagataag
     1981 aaagaatact acaaacttat tacacaagaa actatagaaa acaaacttga atctttagtt
     2041 gaaaatagac tggatacttt ggaagataga tttgaaatac ttaaggaaaa agaaaactat
     2101 cgctatgcta aaaatcgtat tgatgagcta aaaaataaac ttaatacttt gctgtctaac
     2161 cacaaattac ttcatagacg tataatcaaa gcattcccca acataagctc taataagcat
     2221 acagatattt taaataaaat caaagaaaat attaaatcaa ttaaaaaaga taaataataa
     2281 aataaacaca taagttaaat aataaaaata ctaatacata agattaaggt ttagaatatt
     2341 ttttgtaaaa tactctaaac cctaattaat atactattat ttttaatctt aatttataat
     2401 tttagtttat tttactttta acacagtaaa tttaagcgtt taaacttcaa tacactaata
     2461 tactacccca tcaatatctc aactgattct ctaagtgtgc taatctctcc cacatttcca
     2521 gggaaaatta tatatggcat attaggaaat ttgctttctt tgcctgtcat ccatactggt
     2581 ataccaggtt ttatctgtcc cattactgta gcttttttta ctcttagtgc tttagtacca
     2641 acatcactag aagtaattcc acccttagct ataataaaat ttggtcttac attaagcata
     2701 ccaattatac tggtaactgc atctgatatt tctacagaaa tcataagctg tttatcttta
     2761 tcattcgtat ccaagtcaaa acgctctcgt ctagtataaa cagcaacagt tttaccactg
     2821 cttatttttt cctctacaac ttttataact ctttttacct cattttttag accattttcc
     2881 tgaagaacta aatgttgatt aaattctata aattctattg gatatttaca atttttaagt
     2941 tcttctaact gttgggtagt cttattcaca tgtgaaccaa caagtataat tcctccatta
     3001 gtattacctt ttgatactaa ctcatcctta gtaagtagtt ccttgtcact cactccgcca
     3061 agtacttttg ttatggcagc agcgcttcta aacataaatt ccttaccact atttactgct
     3121 cgtataaacg caattgtaaa tacctttaca tctacatagt cggttgcatt tacaataatt
     3181 ttattgaatc cttcagcttt tttaagtttt tctgttatag cttcaatatt tagctctcta
     3241 agttcttttg acgaaatata tatcatatca ctagatttgt actttccttt tgtcttttct
     3301 tcacaccact cacctatatc agatgattta tatccaaaag atttatcttt tgcaaattca
     3361 gtcattcctg ctggtacaag tatttctcct tctttaacat aatgtacatt atctatagta
     3421 aacctaccac cttctttaaa aaatggcatt attatttcac cctgaaataa cttccccgat
     3481 aatctctcta cttcgctttt taataaatta gtttcaagag gataatgtcc tctcatagta
     3541 gaatcactac gacttatgat tataaaatcc ttattaactt tctttgatac atccacaata
     3601 ttctttgaaa tttctttatg tgcttttgta gtttgagcaa cagtaaatcc ccttgaattg
     3661 gtaagtataa aaaacatact atttttctca ttaaaacctt gttccatact atccttatcc
     3721 caatcagtat aaacagaaat atcatgaact gtttgtacac ctgttgggtc atcatctaat
     3781 acaacaattt ttctatctaa atgcttaagc tcatctgcaa gaagtttatt taatctgtcc
     3841 tcatctactt gtttaaaagt attaagtata tctatgctaa ttggttttac ttctctcatt
     3901 taaaacgccc ctttatttaa tttgtatagt ctaaatataa tttatatgat ataaacaata
     3961 gtttgttatt tatagacctg gaagtggtat aattccattt ataacactaa gcaataaaac
     4021 cattataaat gctacaactc cagcaatggt tcctccaatt gtccaagagt ttaaagtatc
     4081 tttaactgat aatccactaa atcttgaaag tacccagaat ccagaatcat ttggaaggga
     4141 gaaaccaact cctccagcac atatagcaag ccctactaaa actggtgaca ttccagttgc
     4201 caaaattgta ggtcctaaaa ttgatgatgt tgttactaag gctacagtag cagaaccttg
     4261 agaaattcta agcaaggcac ttaataagaa tccaagcact acaactgata tactaagacc
     4321 agacatagta tcaactaaga agttacctat accacttgca tttataacac tgccaaacgc
     4381 accacctgaa cctgttatca ataatattag ccctgctgaa tcagcagctt caataactac
     4441 atctcccata ggtttattta tgtatttctt taaaaatcct attgccacca atattccaag
     4501 aagtattgct atgtttttat ctcctataaa tgcaagtact ccactcattg tagaaccttt
     4561 ttctaagaac atactaaata catttgatat aagtataaga agtattggga acaataaaga
     4621 aaacatagaa attccaaaac ctggtacttt agaagaatca tcatcttcaa tttttatgct
     4681 atcttttccc tgttctaaat ttttttctct atcattctca tcaatagcat aagctggata
     4741 tttttcaaaa cgttttccat aaatccatcc acctataaga gctgctggaa aagcaacaat
     4801 tatagaataa agtataaaac tacctacatt tgcatttaca tttgctgcaa ctgctaaagg
     4861 tcctggtgta ggtattacaa gtgcatgacc tactatagtc ccaactccta atgcacatac
     4921 aaaaaccatt agagatttct tagttactct cgaaacatac ttaattatag gcatcattat
     4981 aacaaaagct gcatccataa aaactggaat tgaaattaaa aaacctgctg tagttactgc
     5041 taaagcactg tttttttctc cagtcttagc taacaacccc tttgcaatag attctgttgc
     5101 tcttgattct gaaagaatat ttccaaatat tattccaagt ccaattacaa tacctatact
     5161 acctaatgta tttccaaatc cagtcgatat tgtggttgat atttcattta taggcattct
     5221 aaccataaag cctgtaatta tagatgttat cagtaatgcc aaaaacggat ttactttaaa
     5281 tttaataatc gatacaagta aaattcctat agatataaca aatgctatca aaagtccata
     5341 tcctgtaatc ataatcttta cccctttatc attctacttt attatttatc aattttattt
     5401 ttcttttact atgataactt aacctcgctg tatttatata atctaatcct aataatatgt
     5461 atttaattat attgatatat ttaaatatta attatcgtat ttttttattt caacacctgc
     5521 caacttttca aaatactgtg ctattccact atggtcatct tccaaatgtc catgtacttt
     5581 taatgtctgc ataatttcta ataattgact tgtcataggt agcggtacat ctaaattgtg
     5641 tgctgtttgc attacattct taatatcttt caaattaata gatattttcc cacctggttt
     5701 aaaatcacga ttcataatca ttggtatctt cgcatccaat attgtactac cagctaggcc
     5761 tcctcttatt gccttatata ctttttctgg gtctgcacct gcttttgctg caagtacaaa
     5821 tgcctcagaa actgctgcta tcgtcaaatt aacaattact tggtttgtaa gttttgtaac
     5881 acttccacta ccattatctc caattaaaat agatgagctt cccattatat taaaaaatgg
     5941 ctctactttc ttataaattt cttccttacc accagccata aaagcaagtg taccatcaat
     6001 cgcttttggc tcccctccac ttactggtga gtctataaat tctaagccca tatcttttaa
     6061 tttatttgca cacaatatgg attctgtagg agtaactgaa ctcatatcga ctactatact
     6121 tccttctttt aaagtttttg ccaatccatc catcccaaac aatatatcct gcacaatagt
     6181 tccatttgga aggattgtaa atacaatatc acattcttga cccatttcta aaacacttgc
     6241 gtattttgct ccacatgata taagttcatc cacagcagat ttattaatgt catacaccaa
     6301 aagattacat ccatctttta ataaattttt tgccattggc tttcccatta ttcctaatcc
     6361 aataaatccc aatttcattt tataaccctc catatcttca catttttgaa aacgtttttt
     6421 atgttttaga gtatactaca taccagtata ccggtcaata taaaatataa aagtatttat
     6481 tttaacaata aatagtaatt tttaaagtca tttattatat ttagtcatcc tatataagtt
     6541 ttaatacata ctattgtttt caaatataat atactacttt tacagttttg tattttaact
     6601 aatttcaatt agttttaatt aatctgcctt taatgttaat catgcttcac acttcaataa
     6661 ttttttatat tttttcatct tatctgcaat ttaaattgca aaagattgac cttattttca
     6721 ctatgttgta tgcttaaaat tatataagtt tttgtatttt tatattgttt aggggggtat
     6781 tattaagtat ggctacattt aaaagattac aagaacaagc atatgactac ttaaaagaat
     6841 tgatactatc tggagaaatg atagaaaatg aaatatattc tgaaacaaaa cttgctagtg
     6901 aaataggtat ctctagaaca cctataagag atgctcttca aagacttagt caagatggat
     6961 ttatagatat aatcccaagc aaaggattta gaatccatca aattacagca aatgaaattg
     7021 tagagatatt ccaaatacgc tctgcaatag aaggattttg tactttttta attacaagtc
     7081 aatacaaaga agctagagca gtagaaacta taagtaaatt aaaacatctt ttagataaac
     7141 aaaaagatat tttattaggt gataaaaatt taaactcatt tgcagaatac gatacattat
     7201 ttcacacaac tatagtttca tatgctcaaa atactgaatt tgacaaaatg tttaacaatt
     7261 atatgtacag aataaaaaaa ttagctctcg attcattgtc acatgaagga aggcttgaaa
     7321 caacattaaa agagcacaca gatattttta ataatattgc aaatggatgt attgaagata
     7381 tttacagaac tactctcatt catatggaaa ctcctaaata cattaattta gaagattttt
     7441 gtaacaataa gttattttag tattatactt tcatttatta aaaatatatc tatctcaaat
     7501 taatttccta atttataaaa aatattctat aattatattg taactaatac ttattctaag
     7561 gagtgatttt tttgaacgaa gatgttgtaa ctaatgaaac tattgacaaa gattatgcta
     7621 ttgaagaagt tcgtatggca tgcaaacatt ttggtgattt atatttttat ttttccaaag
     7681 tgttatttga agagtttggt gaagataaaa ctagcgaaat attaagaaaa gttttatttg
     7741 aaaggtctga agaaagagct attgctatga gagaaagagc tcatgaaaat ggagatgaat
     7801 taattgcaga caatattatt agcacaactg atgttccatt tctaggatgg gttcctgaat
     7861 ttgaagagtt acattgcccc tatggagctt cctggctttt aagatttgag gaaaatcctt
     7921 ggtttaaaaa attcgcttct ctttactgtg atgttacaga tactactgta gctgaagtat
     7981 ttacaggaga tacttcccat aaaataacaa aaaacattct atggggagat aaaagttgtg
     8041 aaagaatata tttccatgat gacaaggtat ctgaaggtaa atatacttat ggaaataaaa
     8101 actgtaaata aataatttaa attttctata ttcatatact atattttgta ctttttcata
     8161 taaatattat aactagtctt ttattttact tttgtttaac ctaaagataa tttactttta
     8221 atctatatga attatcatat aaataatcag aaagttatct ttataaatta tagttttaga
     8281 tttataattt ataaaatatt tatgttcttt gtaaaatttt ttagaagctt ctaatgttat
     8341 gctataaaac tatcttgaga agggctgatt gaatgtttaa taaaatatat gaaaagaaag
     8401 aatttatagt ttttcaagtt aaaaaaggtt atgtagttta taatactaga aagagttttg
     8461 aagaaggtca tacacattta aagcattttg aagcagcaaa gacagctatt gatttagcaa
     8521 taaataaaaa gattcctaga agcaaagatg gatattatct gactagttta attcgcataa
     8581 gtgatgatgg ttattacatt gataagttaa gcgaattgtt atatgttaga gaacaaaaag
     8641 gtaaaaaaga aaagtactgt aattctggtt atcagatgta acaagactag ttgtgaatta
     8701 taagtaccaa tcataagtac ctttagtctt aaaatcaaag agtatcagta aatctataaa
     8761 aaaacaactt actgatactc tttttaatta tatgattaat atttgaattt gatacaaaat
     8821 tttatacaaa agtttatgtt atctattctg attctttgtc tgcaaattgg ctattataaa
     8881 gcttctcata aaatccaccc ttttccatta attcatcatg attcccctgc tctataatac
     8941 ttccattatt cataactaaa atcaagtcag cattgcgaat tgtagaaagt ctatgtgcta
     9001 taacaaaact agttctacca ttcataagat ttctcattgc tttttgaagc ataagttcca
     9061 gcctagtatc aacagaactc gtagcttcat caagtatcaa tattgaaggg tctgatataa
     9121 aagctctagc tatggttaaa agctgttttt cccctagaga tatattagat gcttcttcat
     9181 ttaaaaacat attgtatcca tctggaagag tggttataaa gtgatgaaca tttgctactt
     9241 ttgctgcttg tataatttct tctttagtag ccccaaatct accatattct atattttcaa
     9301 aaatggttcc attaaataac catgtatctt gaagcaccat accaaacatt gaacgcaaat
     9361 ctttacgttt catatctctt atatctactc catcaatttt aatagcacct ttctttacat
     9421 cataaaacct catcaataag tttattaatg ttgttttacc tgctccagta ggtccaacaa
     9481 tagctaccat ctgaccactt tttacttctg catttaaatc ttctattaaa gttgaatttt
     9541 ctccatatcc aaaattaaca tgctcaaatg taacatttcc ttttacattt tctatcttaa
     9601 ctggattttc aacatcaggt atttcctctt cttcgtctaa aatttcaaat acacgttcta
     9661 tagctgcaaa agaagattgt attactgtag ataactgagt catttgtgaa agtggctgat
     9721 ttatctgcca tatataacgc acaaatgctt gtaaatttcc tactgttatt cctccactta
     9781 ctgcatatat agaaccgaca actgccacag ctgcaattcc aaagtaagtt actaatgaaa
     9841 ccataggatt catcatactt gatataaatt gtgccttaaa tccattttca caaagttctt
     9901 gatttacttt tttaaattct tttatagaat cttcttcttt accatatagt tttatttcat
     9961 taaatccagt atacatttct tgtactttcc catttaattt tccaagtgca ttttgttgca
    10021 atgaaaatct actttgagat ttttttacta caaatttaga tacaaaataa ctaattggaa
    10081 ttataaaaag agctataaga gccatatata tattaattgt aaacatcatt gaaagtgcca
    10141 aaattatgac taagattgag tttacaattt gcataaaact ctgctgcaat gcatttgata
    10201 ttgtatctac atcatttgat atacgactta atacatctcc aaaactatta ctatcaaaat
    10261 aactaatagg caatcttctt attttatttt cgacttcatt tcttaaatct ctcattgtgt
    10321 tttgaatagc attcgtaagt agaaaacttg aagtatacgt aaaaattgca ttggcaaaat
    10381 atatacatgc taaaatcacc aatatttttg ttacataatc aaaatttacg gaagcccctg
    10441 gtactccttt tgcgatacta attacatctt tagtcaattg tgttattatc aatccttcag
    10501 ttttaggtgc aagtgcattc atagtagctg ctgaaattat acatataata gctataataa
    10561 aggaaaattt atatttcttt acaaatggaa gaagtctacc aattgttttc ttaaaacctt
    10621 tagacttttt cattattcta attcctcctt ttttaactga gatgttgcaa tttcatagta
    10681 tatttcacag ttttttaata attctttatg agttcccatt cctacaactt gtccttcatt
    10741 gagtactata atcttatcag catcaataat agaactaatt ctctgggcaa caatcaatac
    10801 aattgcatct tttgtttcct tcttcaattt agcacgaagt attgcatcag ttttaaagtc
    10861 aagtgcagaa aaactgtcat caaaaatgta tatttcaggt cttcttataa tagcccttgc
    10921 aatagaaagt ctttgttttt gacctcctga tacatttgct ccaccttcac taatcaattc
    10981 atcaaacttt ctaggttttt ttgatataaa ctcatatgct tgtgcaactt ttgcggaata
    11041 ttctaattct gactctcctg ccttatgttt cccaaatcta atattatttg ctatgctacc
    11101 agtaaaaagc agtgattttt gaggtatgaa tcctatcttt tttcttaaag cttttaaatc
    11161 atattctctt atatctactc catttatctt aatgctacct tctgttacat cataaaatct
    11221 aggtatcaag ttaattagag tacttttccc actaccagta cttcctataa aagcgactgt
    11281 ttcacctgtt tttgcagtaa atgaaatatc cttaagtaca gaagcttctc catctggata
    11341 ggtaaaagtt acattatcaa attctatgat acccttattt tcagtatcct taattccatt
    11401 ttcaggattt tttatcaatg gttcttcatt taatagttct tttattctat ttgcagatac
    11461 ctctgctctt ggatacataa taaataccat tgaaaaaagc ataattgaaa acatggcatg
    11521 gaaaagatat tccaaaaatg caaccaactg tccaacttga agctctccaa tatttatctt
    11581 ctcacttgaa atccaaaata cagccaaaac agctatattt aatagtaaaa agaatgctgg
    11641 ttgagtaatt gacataagct taaatagttt cttagaaaca ttagcataat atgtatttgt
    11701 tttttcaaat cgctctgttt cataatcatc atttccaaaa gctctaatta ctcttattcc
    11761 agttaaattt tctctcgata taaggtttaa tttatctagt cctttttgtt gttttgaaga
    11821 tattgcctga gatacttttg ctattattgc tactccaata attataaaag gtactgatat
    11881 agccaatact atagaaagtt ctacacttgt tctaattgtc attattatac ttataatgaa
    11941 cataactgga gttaataagg cagtccttag tagtatatta acaaattgtt gaatttgaaa
    12001 tgcatcattc gttgttcttg taatcattga agaaattcca aatttattgt attcagtatg
    12061 tgaaaattct tgtgattttt caaatatgtc atttcttata tctctggtta cacttgttga
    12121 taccttagca gaacaataac ccaacaatat actacctaca acccctataa tagaaataat
    12181 aacaatcact attcccattt gttttatgta agctatattc tgatttgcaa taccattgtc
    12241 aattatttta gctattatag ttggaatccc caactcgact aatgcaaaac taaatatagc
    12301 cagtatgttt aatactatta ataacttata attctttaaa taacttaaaa ttaatttcat
    12361 atttttctcc tttcttaatt tttgagatag tgtaatcatg taataatact aaatttataa
    12421 atgcttaatt ttataaaaaa aggagaaaag agttaacgca ctctcttctc cagcatattc
    12481 tttttaatac accctccgac aagtcgctta tatgttaaca tatttgacaa gtaatgtcaa
    12541 ttcatatagc caaaagtctt ttaaatgctt catttcataa cattgtaaca aattggcatg
    12601 taatttattt ttgtttttta ctgtattcca attttacaag ttctccaata ttactagctt
    12661 tcttaaaatt caaattagtc ttatctatac cagacttaaa tagtgaaatt cctctcccca
    12721 atattattgg caatatatat atataataat catcaattaa gttatctttc ataaataatt
    12781 ttactatatc gcctccacca actatccaaa tatctttttt atccttctct tttccaattt
    12841 gctcaattaa ttttttaggt tctaaattaa tttatagtgt gattattcta tgtaaacatc
    12901 acttttttac tcattcaata ataccctatt aataaatcct caaactaatt taaataaact
    12961 ttgcataaaa ttaaaaatat attatataaa taatttaaaa ctacactaac ttattgaaaa
    13021 ttctaattat tacaaataaa tattgctctc tgttaacgct agttctataa tataattata
    13081 agctattaat attataaagc cgatattact aatataataa ttataatact tatagttaat
    13141 ttacttttgt tgaaaggtgg taaataacat gaaaatttca gaagtcgcta ctccaaattt
    13201 tttgctagat ttagaccagt tagaaaagaa tatagataaa attcaacaaa tttgtacaaa
    13261 taataataaa caactttggc ctatgcttaa gacacataaa agtacttaca tagcgagact
    13321 tcaaaaaaat gcaggtgcta gtggttttct agttggaaca cttgatgaag gtgaagctct
    13381 gataaaagca ggttttgaag atattatgtt ggcttatcct tacatgggag aagtaaatat
    13441 aaatagaatt gtgactatgg catcaaaaat aaatttaatc tgtgctacag ataatataga
    13501 atgtgctaag gcttatagtg aagctttttc taaatcaaaa atagagtgca agctacttat
    13561 tattgtagat tgtggcttac accgttttgg ggttgaacca gaaaaagtgg ttgaacttgc
    13621 tactgaaatt tctaagctag aaaacatcaa gattgttggt ataagttcgc atccaggtca
    13681 agtgtatggt tgtagttctc cagaaggtgt tccagatgtt tgtgttcaag aagattcttc
    13741 tatggagaaa gcatacaaag ctctaactga taatggattt aatatagaaa ttgtcgcttc
    13801 tggtagtact ccaacagttg aatctgaagc tgcttctaaa actataactg ctgtaagacc
    13861 tggaaactat gtatactatg atgctataca gataggttta ggttgtgcta ctgaagatat
    13921 gtgtgctctt actgttgtgt gcactgtaat ctcaaaaaat agcagtggat actatttaat
    13981 tgattgtgga agtaagtgcc ttggacttga taaaggtgca catggaaata gtagtattgt
    14041 tggttatggt agggtatgtg gacatccaga acttactatt gattcacttt cagaagaagt
    14101 tggaaaaatt aaagtaaatg gtgaaactga tataaaaatt ggtgatagaa taagaattat
    14161 acctaatcat tcttgttcca ctgcaaactt gacaagtaat ttattaggtt ttagaggaga
    14221 tacaatagaa aaagtcatag aagtagatat tcgtgaaaat tcaaaacaaa tcatccttta
    14281 aaaacacatt tattctgata ttaacatgaa agtatatttt tcttaaaatt attgctaata
    14341 actaatgtta atgttggata aataaaaaac ataaattcta gaaatataaa tttaaaagaa
    14401 gaggctgtct caaaatagag ataagcctct ctttcattgt tgaaattatg aaaatatact
    14461 tgatttcata caaaaagagt atctcatgaa ctaaaaagtt cactttgaga tactctcttt
    14521 tttttaatta atatacacat taattactat cttagtttat tactatatat caattactat
    14581 tcattaatcc ttgtatatat taattcttat atatcattta tagttaggtt ataattttat
    14641 gttatttaca ttaatcccat aacagtgagt aaaactctta ctattaacat ccccactatt
    14701 gcatcaaata taggaactgc caacagtaac attctatact tagtactggc atttgctaca
    14761 agcactattc tggcataatg accaactaga gtacccatta acatacatgg cataattaat
    14821 atggttgctt gtggtgcagt gattagacct tgtgcaaata agtttgcagc tgtagctgct
    14881 cctgctgcct tagcaaagaa tgctgctatc aaaactacaa cagattcccc aggtagtcca
    14941 aaaataccca ttactggtcc aaaaatattt cctaaaatat caactaaacc agttaattgt
    15001 aaaaactgca ctattacata acccattatc atggctggta atatctgttc aacacctatg
    15061 taaaaacctt tttttgctcc ccccataaat atctcaacag cagttttctt aggtttacta
    15121 tttacaattg catttccagt attttgttga ctcatactac acagcctcct tagccttatt
    15181 ctttttattt cttacaccta tgattaatct aactagatta gcaccaagta ttttacagaa
    15241 tatctctata aggattattg gacctaatgc caataatgct attggtaaaa gtggagcttg
    15301 agtatttatt gtgttcagta taacagctga accagcatat tgatatgcta caaatatagt
    15361 tctttcatca tctgttattt ccccactttc aaataattct ttcgtcatta ttgaggctac
    15421 atctgaacta gtaaatgaag atacaaatgc tattcctgct attccaggta taccaagcag
    15481 aggtctaagt aatggattaa atatctttga tgctgctttc attcctccca tagattcaac
    15541 tacatctata agacctacag caaaacagac agttggaatc aaagtcagag caaataagaa
    15601 accatctttt gctcccgttc ctcctttacc cataaagtct acaccatctg caatagtacc
    15661 aaatgaacct gtcagattag caaaatctaa tgctttaagg aagccttgac tatctttaaa
    15721 cactcctgaa aataatacta tcaatacaaa taaagataaa taccctttcc atgttacctt
    15781 attatctcca gctgttacat ccatataaaa tcccccttta tacctcaaat ttatctaagt
    15841 ttaaaataac tgtagaaatt aatttcaccc tctcaaacat agaatctact actgcatatt
    15901 ctcttgatgt gtggttccat tcccctctta caccaaatga acagattgta ggaacgtttg
    15961 caatagtcaa ataactagca tctgaactac ctccaagtct cttagcattt aattctccaa
    16021 atccattttc ttttgacact tcatttacaa atgtatgaaa tctcataaca tcttctgtag
    16081 tttcaaatgg catcatttca cttataaaat taacttctgt agaagttcct tctatgtaag
    16141 tttctttaca tatctctcta acttgttctt taacattctc catttcttct actttttcaa
    16201 atctaaggtc tacttttata ctacaatctt ctggaataga attttccact cttccaccct
    16261 taatcatact tacacttact gtagttccaa catctaaatt agtcaatttt tgtatgcgta
    16321 gtatcttatt agccatttct tctatagcat ttcttccacc ttcaaaatca ttacctgcat
    16381 gtgtttctac accttttaca tgtatattac atccgattct tccttttcta cctacacaca
    16441 aactattatc tactaatcca gtttccatat taaaagcaca aagaccacct tttgcttccc
    16501 tcaatataac atctgcacct gttgaatcct tatgtcctat ctcttcatct ccagaaaata
    16561 ctatttttat tggtctttct ttgtatccta ttttatttaa tgctttaata gcatataagg
    16621 aaattattat accacccttc atatctaaaa cacctgggcc atatgctttt ccttctatta
    16681 ttttaaatgg attctctcca aatgtacctg tttcaaagac agtatccatg tgacctgaaa
    16741 aaattattgg ctttttatct atattagaac caagtgtacc tatcaatgta ctaccattat
    16801 ctccaatatc tactaattcg caatctaaac cttctttttc aaattcaagc tttaatctct
    16861 cagctaactt gtttacactt tctttacaat gtgtatagct ttccatatta acaatctctt
    16921 tccataatga aactatttct tctctattct catcaacaaa cttagaaatt tcatttaaat
    16981 atgtattcat aatcttctcc tttattttca aataaaatgt atttctttcc taaaacctaa
    17041 gtttatttta ggaaatctta aaggactagt ccttaattac aaaatagcac aattattctt
    17101 gccagtcaac atatttttca gtctttttta tattttctga tttttctttc aattatcctt
    17161 ttgtaaaata aattgacgcg ttttatctac tattataaaa tgaggttata aattgattga
    17221 ttagttacaa tactctcgaa attttaaagg aggttttatg aaatataaaa ttggtttttt
    17281 gacaacatct tattttatgt cagataagtt taaacaagct cttaaagaat taaatgatac
    17341 ttgtgaaatt acatttattg agctcaaagg ggagaatcct tttaataatt tacctaatat
    17401 atactatgag caagtagata attttgatgg atttgtatgc agtggtatag ttccatatag
    17461 tgaacttata ataaatataa aagacataaa agttccatta aattttttaa agcttgatga
    17521 aagagatttt tataaatact tattcaaatt attaaatagc caaaaagata ttgactttac
    17581 aaaatcattt atggattttt taagagagga caacaattat tatgatatat attcattaat
    17641 agactctaat aaatgtccat atacaattaa agattttaat atccctcaac ctgtatatga
    17701 ctttaaaaaa ttaaatgata aattattgga aatacattta gacttattgc gagataataa
    17761 gattaacctt tcttttacta gaaattacat cattagttgt gaacttaata agcttggata
    17821 tgattgtatt tacatggttc catctacaga atcaatcctg aatacattta aatctctggt
    17881 gaatgaaata acattacaaa acttagacaa aaataaatct gcaacttgta tagttacagt
    17941 aaatagttca gagtacataa atgaagattt gatatttaaa aatgatgaga tacaaaatca
    18001 aatatataac actatattaa attctctatc aagacatgga ttctatggag ttcaggtcaa
    18061 aaaaaatgat atgaaaatag agatacacac tactaaagaa atgttaaaca atatgaccaa
    18121 taactataca gattttttta tttcagaaga tttaaaagaa attaaatata gcttaaatat
    18181 aggttggggc attggaaata caaatattca tgcagagcaa aatgctcgtc aagctaatgg
    18241 taaatctgct tctttgaatg gaaactgtac ttttatagta aatgatacta atgatacaat
    18301 aggtccttta tattcaaata aaaattctaa taatattgaa gatagctcaa tagccaataa
    18361 agttgcttct tttatacctc tatctaatac aaatgtatct aaaatcatgt gtatgattaa
    18421 tgatagaaat tcaaataatg tgtcagcaga aatattatca gactatatga acataactct
    18481 tagaagtgct aatagaatat tgagtatctt atataaagca ggaattgcaa ctattgtaaa
    18541 tacaaaactc gataatcaaa gaggaaggcc caaaaaaata tataaagttg atttcttatc
    18601 ttttctaaat aagatgcaga aattaaattc ttaatattta attttttaat aaaagcaatg
    18661 tattttctct aattttaaag aatcaaaatg tttaaatttt attagaatat acaacttttt
    18721 actttaaaat acctaaacct tttatcagtg aaataaacca taatatatcg taacttatta
    18781 gaaaaacaaa ataatttcat atacaaataa taaaatagcc actacaatcc ataatttgc
//
LOCUS       DBGJFF010000071        18790 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.072, whole genome shotgun sequence.
ACCESSION   DBGJFF010000071 DBGJFF010000000
VERSION     DBGJFF010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18790
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.072"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(<1..591)
                     /locus_tag="ACOIAF_002445"
     CDS             complement(<1..591)
                     /locus_tag="ACOIAF_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HGZ2669452.1"
                     /translation="MLNETRKKQLTEVCQDLIRNASYSGQEENVVKAIEENFALLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEARIVDGKIYGRG
                     TSDMKGQVSAMMAAVSYFAEDTNKDFEGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKG"
     gene            complement(924..2138)
                     /gene="dpaL"
                     /locus_tag="ACOIAF_002446"
     CDS             complement(924..2138)
                     /gene="dpaL"
                     /locus_tag="ACOIAF_002446"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HGZ2669453.1"
                     /translation="MREIKWKLNNLPKSEEKEKGIEFLKDEEIAKAKAFHESFPQYEK
                     TPLVKLDNLAKLLGVSGIYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLNTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAKEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYNECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNYSSAFVSAPDWVSAKGMRILGNP
                     LRGDEKVISGESGAVTTGALVSILESEDLKDLREALKLDENSKVLLISTEGDTDPDKY
                     RDIVWNGECQSK"
     gene            complement(2770..3309)
                     /locus_tag="ACOIAF_002447"
     CDS             complement(2770..3309)
                     /locus_tag="ACOIAF_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HGZ2669454.1"
                     /translation="MQYAIELYYDKKTEKQLFDLSKKIADEKISTKYLEWKTRPHLTL
                     ACFNDVDEACCIEKLKGFAQNHKVIPAYIGSVGMFNDTKTVFVSPIMNSSMYQFQREL
                     YEVLQDFDSNGWEWYHPNRWVPHCTIALTGDDESEAFFRASDVLLREFKKISGEFVSI
                     GLVKITFPVEEIFTVNMNR"
     gene            complement(3386..5191)
                     /locus_tag="ACOIAF_002448"
     CDS             complement(3386..5191)
                     /locus_tag="ACOIAF_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HGZ2669455.1"
                     /translation="MLSKLKEFQQEMIKYTETVASVLDVDIEIVDDRLIRISGTGLYK
                     SKINESVVTEGFIYDNVIQTGQELVVLDICDNQLCIECSHYMKCLNKVIIAVPIKYNN
                     RTIGVIGAISTDKTKKVEISAKIDNYLKFVNHICDLISMKIEEHEASKNSSRKMDMMI
                     EIIENVEKGVIILDINSKISYINNIALKKLDIDKNIIENIVNIVSVESSSNGHELLEI
                     DIDNKIYNINAKIIPVYPYINQYDKIIIFDKTYINHKGHGKVNSGWGNSDIESIIGNS
                     EAMLKVKERTKKLAKSNSTVLITGESGTGKELIARAIHAEGSRWNKPFIAINCAAIPE
                     NLLESELFGYIKGAFSGASSGGKVGKFELANEGVIFLDEIGDLSMPLQAKLLRVLQER
                     KFARIGSNKLIDLDIRVIAATNKNLLKLVNEGKFRDDLYYRLNVIPINLPPLRERKDD
                     IEAIMMKFASKYSLELGIQLNKIEENVMNMLINYNWPGNIRELENAVEYMMNLVGDDG
                     IIYKDMLPLDILNYYNINGNIYKNKDVNIIFEDDIVGGIVENQERILSIKELELTYIN
                     KLLNKYGRDTKTKKKIAKDLGIGLATLYRKLEEEQ"
     gene            complement(5587..5964)
                     /locus_tag="ACOIAF_002449"
     CDS             complement(5587..5964)
                     /locus_tag="ACOIAF_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HGZ2669456.1"
                     /translation="MSKVVIATEKAPGAIGPYSQAIQVGNIVSTSGQLPIDMKTGELV
                     SDPKEATKASLDNVKAILEQAGTSMDKIFKTTVFVKDLKDFNDINEVYATYFKENPPA
                     RSCVQVAQLPKDAVLEIEAMAVL"
     gene            6862..7347
                     /locus_tag="ACOIAF_002450"
     CDS             6862..7347
                     /locus_tag="ACOIAF_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669457.1"
                     /translation="MSKSELTAETTEEMLEVLSGKDYDIACHLHELGKSLDCKIEPKT
                     GARSYKIVYSTKKPKRSLFTIECNEKKWRVKANLFHLNTYKDAVEECSKTIKDSITKT
                     RTCKKCNSKCIGGSSFELDGKSYLTCIGSGHYFANMEEMDWKNLEKLITKENNIMQES
                     V"
     gene            complement(7567..8106)
                     /locus_tag="ACOIAF_002451"
     CDS             complement(7567..8106)
                     /locus_tag="ACOIAF_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HGZ2669458.1"
                     /translation="MSKNVLIVSASPRKGGNSDLLCDQFIQGVLESKNQVEKIFLKDK
                     DINYCTGCGACYEKEANCSQTDDMGEILEKMIGADVIVLATPVYFYTMNAQMKTMIDR
                     ACSRYTEICDKDFYFIVTAADENVADMERTIEGFRGFTSCLNGSKEKGIVYGVGAWNV
                     GDIKGSKAMKQAYEMGKLA"
     gene            complement(8099..8512)
                     /locus_tag="ACOIAF_002452"
     CDS             complement(8099..8512)
                     /locus_tag="ACOIAF_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HGZ2669459.1"
                     /translation="MTISEVSKKYELSADTLRYYERIGLIPPVNRNKSGIRSFTEEDC
                     EWVNFIKCMRGAGLSIETLIEYVAMFQQGSSTIKARKELLIEQRNQLAKRIEEMQKTL
                     ERLNFKIDRYEEGIIEKEKVLKSSRNKKMEAVSYE"
     gene            complement(8548..9669)
                     /locus_tag="ACOIAF_002453"
     CDS             complement(8548..9669)
                     /locus_tag="ACOIAF_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HGZ2669460.1"
                     /translation="MEAKKLGFGLMRLPIKDENDVTTIDMEHLNKMVDTFLERGFTYF
                     DTAYVYHMGKSEIALRESLVKRHKRESFTIATKLPLMALKKKEEQETIFNEQLEKCGV
                     DYFDYYLLHNIGVSHYEVAKKFDSFKFIEEKKKEGKIKNIGFSFHDSAELLDKVLSEH
                     PEVDFVQLQINYLDWDNESIQSRKCYEVAEKHNKPVIVMEPVKGGTLAKIPEKAEKLL
                     NDYNPDMSISSWAIRFAASKDNVMMVLSGMSNMEQLLDNTGYMQNFKPFVQEEYNIID
                     EAVEIINESILVPCTACQYCVEGCPKNIAIPNYFALYNAEKQALNTGFSTQMVYYNNY
                     TKTYGKASDCIECKQCEESCPQHIKIIDALKNVAETFEK"
     gene            10268..11920
                     /locus_tag="ACOIAF_002454"
     CDS             10268..11920
                     /locus_tag="ACOIAF_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp21"
                     /protein_id="HGZ2669461.1"
                     /translation="MNKKNLAILISGMMFFSSSSAIFADNTTKQERLIGKTRYETAVE
                     VSKLGWVQSKTAIIVNGNSIQSALCANPFAKLKNAPILLVNNNSIENSTKAELKRLGV
                     DNVYIVDSGNSISSKVENEIKSLNIKINKIVGNNIYEMSTNVLKEIDKIKKVENVAVV
                     NGLKGLSDAVSIASPAAIHNMPIILVSDENSNDSSINFLNNRSIKKSYIVGGNKILSD
                     NTVKKYPNRERISGSDRNNTNAKIIDKFYKDKKINNVFVAKDGINQENALVDSLSIGV
                     LAAKENSPIMISNGSLVNSQKEFIKNKDIDEITQVGGGYNQKAFEDILKLKNVNIRNK
                     TKKISLIQAKEILKQALPGSNILVCYYDEDDNEYDCKVIKDNILYEIEIDAYSGKIIE
                     IEKDDDLNDNNLGTQNISLDKAKSIALDAVPNGNLIYCKYDAEDNEYEAKVIKNKKTY
                     ELEISAFDGRIVDIDMDNDSDDDFDDNDKNNSNTNDNSSIISAEKAKSIMLDKVPGGK
                     IVKFKYDEDDKEYEGEIIKGKTEYEITINAITGKILEFEIDD"
     gene            12219..12968
                     /locus_tag="ACOIAF_002455"
     CDS             12219..12968
                     /locus_tag="ACOIAF_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005340256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2669462.1"
                     /translation="MNTILKVNNITKTYNKGQQPSLDKVSFNVSEGEFIGIMGASGSG
                     KTTLLNVLSTIDKPTSGDIFINNINLKSLNDTCAADFRKDNLGFIFQEYFLLDSLTIF
                     ENISVPLTLQKLNPDKIENMVKSLAERFGIASQLNKYPNELSGGQRQRASAVRAIVKH
                     PSILFADEPTGALDSNSATELLHSLQEANEELKTTILMVTHDAYAASFANRILIFKDG
                     HIIKELNKQQNNRHTFYELIIQEIAKLDTKR"
     gene            12981..15008
                     /locus_tag="ACOIAF_002456"
     CDS             12981..15008
                     /locus_tag="ACOIAF_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HGZ2669463.1"
                     /translation="MNIFYTAIKNLKKNFSFYSLYLLSVSFVITVFFAFTSFSVNKIM
                     LEKISTDGRVESMCNVISIFLMAFVIFYMAYSNRFFLRRRTKELGIYALLGYRKFTIL
                     SLLTFENILICSGAFIIGLALGALAHKGIVFGITALLNLSIDNSQIPFFNINAINKTI
                     IFIALVVLVLAVSNGKFLYKTSLVKLIRYEKSAEKKLKCRKIPAFMGFIMILSGYCLS
                     LDILRGAKSVWVSIGFYQMGLITMIIIVIGTVLFINFFLPYIMQKSKKNKRTFYTDMK
                     IIAIPNFIYRIRSNSKTLIMLTLLSATTLTVSSVMALSLYYPIASVSRIAPSEIEFRV
                     EKNKQIEDVKQIVSQYTSNTDDIIFTQTNIYKVKSSSKDLPFEYEIGTSKNNLSNSSS
                     SKEVISRESGFECISFSDYYTLLKAQNRTKLLKQLTPLRDNECILMKYQPNNDGYSEN
                     GTIYPLLVNGKETPVAVRETSLNNAISFANSVGTLIVNDSIYEQIAQNKLPMTSILSI
                     NGKSIKNNENLYSNISDYLDGNPYLQGYSHRINDLLSINSSTFLLIGFLVVLFFIAVG
                     SILYFNNISAISDSTSDYKILQKMGYDNIQIKKIIKKQVLPFFNIPFLLGILDCIFAT
                     LVYKTALMQNLLGNSFSQYIPVIIAIFLTSVVYLIYYILTIKSCCKIVLKN"
     gene            15152..16735
                     /locus_tag="ACOIAF_002457"
     CDS             15152..16735
                     /locus_tag="ACOIAF_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGZ2669464.1"
                     /translation="MTNFNLKILFLGNYILSLLFMGISTSTETYMNIQSIIPLIISTL
                     LLIDITYITSCNVRENNIISLFCGLLALDSWYLLLSFVTTPIGTIIFKILSTVIVYVS
                     IKFIFLFLFQGSGYNFRKITDILLLITCIGAIVGAFISNRTYACMYGIQFVVSIISFF
                     YIIFYHLKRVVFVIKSEKKPISISVFITIIGFITYYFFTLRISNHIGNFGIYLTVLVF
                     FMSIHGIALKENDSFPISTVFNIKQRAIFLFISILVISLVVLFANGNFSIILIAINVL
                     FMLIFLCNIILEFNLRDKKNKVVKNSKYTFALEKLQQEEQLKTEFSNFLHDDILQNLL
                     SIKNMMSKSYRPEVQDIIYETLNNLNTCIRKQMQDYHPVILKNLTVKENYQNLIESVS
                     SSFPQKNICVSFKCSDTLFLVEPYDILIYRLIKELLTNVYKHSNGSHAWIALALEKNI
                     IKLNINDNGVKNTSFKTPLDITKITFDTIKHKGIISIKEQVENLGGTISVSDNIPHGV
                     CVQITIPMKGDGSYKYFVS"
     gene            16743..17327
                     /locus_tag="ACOIAF_002458"
     CDS             16743..17327
                     /locus_tag="ACOIAF_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HGZ2669465.1"
                     /translation="MLFAKSLSIALEEYKEIEQFYSTQDIHNLSEMIQDKKIDIVLMD
                     INLRGLSECDGLKVAYNLLETNSTIKIIILTGYDLPVYKHEAKKIGIYGFLNKNISPD
                     SLIDSLLKVYQGGTCFPSENTEVIIEDLTNMEKRILQLICSGKKRKIIADELYISERT
                     LSNHLQHIYDKLDVSSSVEAITKAIKMGYISPVY"
     gene            complement(17510..18436)
                     /locus_tag="ACOIAF_002459"
     CDS             complement(17510..18436)
                     /locus_tag="ACOIAF_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HGZ2669466.1"
                     /translation="MSNSFYQLNSAVLYFNEKRDVNRIRFNHYVMRKENIQRLKGTLP
                     RGQFMMTVRKAASDLDVSISTISRLVNEFIDLGILRLISRGVKGNCCSVYSYVCSESS
                     NAEIVTKNGDIFKNIEKYIDATINNNQKTMKYKHRNELFKNSNTNTYSRINQESIDKD
                     YDFIIHTGYETENETKKKELLKNNLNKKNLNKNTFENSDEDLTEDSTYKNPESIHEII
                     IRKLNNESGKNFKADLSITKALIDKRLKEGYSLEDFYKVIEAKVRRWRYTDMEIYIEP
                     TILFGYEFESYLNEESSLSACNYGDMCYEDMY"
BASE COUNT         6449 a         2929 c         2241 g         7171 t
ORIGIN      
        1 acctttttct ggattagcag agtgagctgg tttaccaaaa gtttctacta ctatttctcc
       61 tctacctctt tgacctatct ttaggtttaa ttctgaagat tcacctatta caacataatc
      121 aggttgaaca gccttactta tctctctagc tgaaacacct tcaaatattt cttcatgaac
      181 aacacctgct acatacaatt caccttcaaa gtctttgtta gtatcttctg caaagtatga
      241 aacagcagcc atcatagcac ttacttgccc cttcatatca gaagttcctc taccatatat
      301 tttcccatct actattctcg cttcaaaagg aggaacactc catttacttg catctggaac
      361 tggaactgta tctatatgcc cgtcaaataa tattttctta cctggcttgt taccttttat
      421 acatcctact atatttccat atctatctct tgaccaacta tcaaaaccta gtaaagcaaa
      481 attttcttct atagctttta ctacgttttc ttcttgacct gaataactag catttcttat
      541 taagtcttgg catacttcag ttaattgttt ttttcttgtt tcatttaaca ttttgatatt
      601 cccccatata atttttaatt tgttgacgtt ttattttgat atttcacttt tttaatttat
      661 tagcatttca cttaatctat tgatttttta tttctttata ttttatttat taaattttta
      721 tttagacttt attttaattt tatttggttt tatttaattt tatttagttt ttattttgaa
      781 tttttcattt gctttacttt ttcatttatt tatatttttc atttgtttta gtttttattt
      841 tatttaaagt tttcacttat ttaagttttt attttcctta aatttttcat ttatttgtta
      901 agtttttctt ttccttaagt cttttattta ctttggcatt caccattcca aactatatct
      961 ctatatttat cagggtcagt atctccttct gtacttatta ataatacttt agagttctca
     1021 tctagtttca aagcttctct taagtctttt aaatcttcac tttcaaggat tgaaactaat
     1081 gctccagttg taactgctcc agattctcca gagattactt tctcatctcc tcttagaggg
     1141 tttcctaata ttctcatacc tttagcagat acccagtctg gtgctgaaac gaatgctgat
     1201 gaataatttt ttaacacttc aaaacctata gtgtttggtt ctccacaagc aagccctgcc
     1261 attatagttg gcatatctcc tccaacaaat cttggttttc catctccagc ttctgctgat
     1321 ttatagtaac aatcagcctc atcagcctct actactactg ttattgggca ttcattatat
     1381 attgaagcaa catacccttg aactgcacca gcaagtgaac caactcctgc ttgaacaaat
     1441 acgtgtgttg gtctatctac tccatattct tttaattgtt ctatagcttc tttagccata
     1501 gttccatatc cttgcattat ccatgctggt atttcttcat atccgtccca tgctgtatct
     1561 tgaaccatta ctccatggtc atcagcttct gcataatcat tagctaatct tactgcatca
     1621 tcatagttta agtctgttat actagcatct gcaccttctc ctttgatttt atttaatcta
     1681 aattcagaag aaccttttgg cattaataca acagattttt gacctaattt attagctgtc
     1741 caagcaactc ctctaccatg atttccatct gtggctgtaa agaaagttat ttctcctaat
     1801 ttttctttta tttcttttga agttaattta tcatacccta actcagatat atctgtattt
     1861 aatctttgag ctaagtatct acccatagag taagaaccac ctaaaacttt aaatgcgttt
     1921 aagccaaatc tataagattc atctttaaca tatattccgc ttacgcctag taattttgct
     1981 aagttatcta atttaactaa aggagtcttt tcatattgtg gaaagctctc atgaaaagct
     2041 ttagcctttg caatttcttc atcttttaaa aattcaattc ctttttcttt ttcctctgat
     2101 ttaggtaagt tatttaattt ccattttatt tctctcattt attttatatt cccctttcaa
     2161 atttttcggt gtcattttaa ttttttatct ttgtgcctta tagcaaagca attaccgtgc
     2221 caatattatt tattctaaaa acaatttttt tatgttggaa ttacatactt ttataaaatt
     2281 tctttaaagt ttataaattt tgagtgtgaa aaataagatt tataaaagaa aaaattctca
     2341 tattgggaaa tttttatcaa tacgagaatt atcaattcga gaaatcgttt atttatctta
     2401 attcttcctt tattatgctc ttatttttta tatggctatt gttttgtcag atatattact
     2461 acttttattt tctataaata tatggctata accttcttac gcataaactc tataaattta
     2521 tatactttat atactttata ttctttatta cccaaatatt ctttattacc caaagcctcc
     2581 aaaatggctt aaatcttttc atagcttctt acagaagttt acatggtcgt ccccccaata
     2641 agcaaaagtg caatatgtgt accattgtgc cctacttgcg actattgagc tgtcgaaatt
     2701 cgcaacttcg catttatctg atgaaatgca ctatgcaaaa tattcataac atccacaaca
     2761 gagttaaagt tacctattca tattcactgt aaaaatttct tccacaggaa atgtaatttt
     2821 aaccaatcct attgagacaa actcaccact tatttttttg aactcacgca gtaatacatc
     2881 acttgcccga aaaaaagcct cactttcatc atctccagta agtgctattg tgcaatgggg
     2941 tacccaacga ttggggtggt accattccca accatttgaa tcaaaatctt gtagtacctc
     3001 atataattct ctctgaaact gatacatact gctattcatt ataggtgata caaacaccgt
     3061 tttagtatca ttaaacatgc ctactgaacc aatgtaagca ggtattacct tatggttttg
     3121 tgcaaacccc tttagctttt ctatgcaaca agcctcatcc acatcattga aacaagccaa
     3181 agtaagatga ggtcttgttt tccattctaa gtacttcgta ctaatttttt catcagcgat
     3241 ttttttagat aaatcaaaaa gttgcttttc cgttttttta tcataataca gctctatcgc
     3301 atattgcata ataacacctc caccataaag taaaaatacc tatctataaa aacacacaaa
     3361 tacctatcat tttaatatta tattactact gttcctcttc caattttcta tatagtgtag
     3421 ccaaacctat gcctaagtct tttgctatct tcttcttcgt ctttgtatct cttccatact
     3481 tattcaacaa cttgtttatg taagtcaact ctaattcttt aattgaaagt atcctttctt
     3541 gattctctac tatccctcca actatatcat cttcaaatat tatatttaca tccttatttt
     3601 tataaatatt tccattaata ttataatagt ttaaaatatc taatggaagc atatctttat
     3661 atattattcc atcatctcca accaaattca tcatatattc aactgcattt tccagttccc
     3721 ttatatttcc cggccaatta tagttaatca acatattcat tacattttct tctattttat
     3781 ttaactgaat acctaattct aaagaatatt tagaagcaaa cttcatcatt attgcctcaa
     3841 tatcatcctt tctttcccta agaggtggca aatttatagg aattacattc aatctatagt
     3901 ataaatcatc cctaaactta ccttcattta ctaatttcaa taagttttta tttgtagcag
     3961 caatcactct tatatctaaa tctataagtt tatttgaccc aattctcgca aattttctct
     4021 cttgtaaaac tctaagtaac tttgcttgta aaggcattga caaatctcct atttcatcaa
     4081 gaaatataac accttcattt gcaagttcaa atttacctac tttacctcca ctactagctc
     4141 cactaaatgc accttttata tagccaaata gctcactttc aaggagattt tctggaatag
     4201 cagcacagtt tatcgcaata aatggcttgt tccatctgct accttcagca tgtatagccc
     4261 tagcaattag ctctttaccc gtaccacttt ccccagttat aagtacagtt gaatttgatt
     4321 ttgctaattt cttagttctc tcttttactt ttaacattgc ttctgaattt ccaattatag
     4381 actctatatc actatttccc cagccactat tcacttttcc atgtccttta tgattgatat
     4441 atgtcttgtc aaatattatt attttgtcgt attgatttat atatggatat accggaatta
     4501 ttttggcatt tatattataa attttgttat caatatcaat ttctagtaat tcatgtccat
     4561 ttgaggaaga ttcaacagat actatattta ctatattttc tattatattt ttatctatat
     4621 ctaatttttt tagagctata ttgtttatat aagatatttt gctatttatg tctagtatta
     4681 taacgccttt ttcaacattt tcaatgattt ctatcatcat atccattttt ctactgctat
     4741 tttttgacgc ttcatgttct tctattttca tagaaattaa atcgcatata tgattgacaa
     4801 attttaaata attatctatt tttgcactta tttctacttt tttagtttta tctgtactaa
     4861 tagcaccaat tactcctata gttcgattat tatattttat tggaactgct attattactt
     4921 tatttaggca tttcatgtaa tgggaacatt ctatacaaag ctgattatca catatatcca
     4981 atacgactaa ttcctgacct gtctgtataa cattgtcata aataaatcct tctgtaacaa
     5041 cgctttcatt tatctttgat ttatacagac ctgtaccact aattcttatt aatctatcgt
     5101 caactatttc aatatctaca tctaacacac tagctactgt ttcagtgtat tttatcattt
     5161 cttgttgaaa ttcttttaat ttacttaaca tagtttcccc cattagaatt taatttacca
     5221 aattatcagt ttagctatat tttaacatat agtcactctt taaaatatat ataaaacatt
     5281 acttatttat atatttgcgt taaaacagac aatattaatt tataaaaagt ttgttttata
     5341 tagtaacctt aattataata cacagacaat aaaataaatc acaatacaat aataaaaaaa
     5401 agctttcctc atataacaaa tatatttttg acataaaaat atacattata tgagaaaagc
     5461 tcaatagtta aatatttaga tagattttta tatagacttt tttaatataa acttttttaa
     5521 ttgtagactt tttttattag tttttaattt ttattagttt tgttattggt tttattgtta
     5581 taatacttat aatactgcca ttgcttcaat ttctaaaact gcatcttttg gtaattgagc
     5641 aacttgtacg catgaccttg ctggtggatt ttctttaaaa tatgtagcat atacttcatt
     5701 aatatcatta aaatccttta aatcttttac gaatactgta gtcttaaata ttttatccat
     5761 acttgtacca gcttgttcta atattgcttt tacattatct aaagaagctt ttgttgcttc
     5821 ttttgggtca gatactaact cgccagtctt catatctatt ggaagttgac ctgatgtaga
     5881 aactatgttt ccaacttgaa ttgcttgaga atatggtcca attgctcctg gagctttctc
     5941 tgtagctatt actacttttg acatgacttc acctcctatt attaagttat atttacagga
     6001 aaataaaatt tcctaaacac caatattaat acgaaccttt agattaaatt tcaaatctaa
     6061 aacttcaaat aatcaatact tagatgatta tgtttatttg tgtcttgtta acttttatta
     6121 aagcaattaa cgtgccaaac tttatttata tataaaattt tgtgaagtca ttcaaaatag
     6181 ttacttctga attttttcgt aaaaaaatgc tttaaaatta cttttttata atgtcaattt
     6241 ttttctcata tctagaaatt taccaacatg agaattcttg ttttgagaat ttgcagatta
     6301 aagttatttt ttaaccttaa attctgaacc tcccatgact aaagtcacaa gtgcgcgtag
     6361 ctatttttta atcaagttat tacatatgaa agaactatag ttgacaatcc cactattata
     6421 aataataaaa cctttaccca taccttattt accctagtta tcatagagcc tattgaaaat
     6481 ataacagcta aatataataa taaccaaaaa attatcaata tctaccactg ttgctgaaaa
     6541 tccaaaaaac taccttttga tagtcttact taacaatcta attaatctca taataaattt
     6601 acaacttata aataaattgt tatattggcc gtatctcaac aactttctcc tttcctagtt
     6661 ttaaatcttt tatagaattg taccacgtct aagtaaattt tcatactatt aagtcttatt
     6721 tctaataaat cattagctct cattctatgc ccaataatgt aagattgcag taattttatt
     6781 gattttcata gttttattta atatacttat attaattatt ttcatatgaa attattattt
     6841 atataggggg atttttttaa tatgagcaaa agtgaattaa cagcagaaac aacagaagaa
     6901 atgttagaag tactaagtgg taaagattat gatattgcat gtcatttaca tgaacttggt
     6961 aaatcattag attgtaaaat tgaaccaaaa acaggtgctc gttcttacaa aatagtatat
     7021 tcaactaaga aaccaaaacg tagcttattt actattgaat gtaatgaaaa aaaatggaga
     7081 gttaaagcaa atctttttca tctaaataca tataaagatg ctgtggagga atgctctaaa
     7141 actattaaag atagtataac taaaactcgt acttgtaaga aatgtaattc aaagtgtatt
     7201 ggaggttctt cgtttgaatt agatggaaag tcttacctga cttgcatagg aagtggtcat
     7261 tattttgcaa atatggaaga aatggattgg aaaaacctag aaaaattaat tactaaagaa
     7321 aataatatta tgcaggaatc tgtatagttt tttaagggta attattaaaa tatatgagta
     7381 acaacagata cttaaatagg cataaaaaaa taaagggtat aataaggata tagaataaaa
     7441 ataaatgata tagcacaaag aggctgtctc aaaatacaga taagcctctt tttattgttg
     7501 aaatgataag aatatattca attttataca attttattca attttattca attttataca
     7561 aatatactac gccaacttac ccatttcata tgcctgtttc attgccttac ttcccttaat
     7621 atcacctaca ttccaagcac ctacaccata tactatacct ttttccttag aaccatttag
     7681 acatgatgta aatcctctaa atccttctat tgttctttcc atatcagcca cattttcatc
     7741 tgctgctgta acgataaaat agaaatcttt atcacaaatt tcagtatatc tagagcaagc
     7801 tctatcaatc atagttttca tttgcgcatt catagtataa aaataaacgg gagtcgctaa
     7861 aacaatcaca tctgccccaa tcattttttc aagaatttct cccatgtcat ctgtctgtga
     7921 acaatttgct tctttttcat aacaagcacc acatccagta caatagttaa tatctttatc
     7981 ttttaaaaat atcttctcca cttgattttt agattctaat accccttgta taaactggtc
     8041 acacaataaa tcagaatttc caccttttct aggactagct gatactatta atacattttt
     8101 actcataaga tactgcctcc attttcttat ttctactgct ctttaatact ttttctttct
     8161 caataatacc ttcttcatat ctatcaatct taaaatttaa tcgttccaaa gttttttgca
     8221 tttcttctat tctttttgct agttggtttc tctgctctat taaaagttct tttctagctt
     8281 tgatagtact gctcccttgt tgaaacatag ctacatactc aattaatgtc tcaatagaaa
     8341 gacctgcacc tctcatacac tttatgaaat taacccactc acaatcttct tctgtaaaag
     8401 acctaattcc acttttattt ctatttacag gtggtattaa accaattcgt tcataatatc
     8461 gtaaagtatc cgctgaaagc tcgtattttt tactaacttc tgaaatcgtc atacatatct
     8521 tctcctcttg atattgtata gattgaatta tttctcaaag gtttctgcta cattctttaa
     8581 agcatctata attttaatgt gttgaggaca gctttcttca cattgtttac attcaataca
     8641 atctgaggcc tttccataag ttttagtata attattatag tagaccatct gtgttgaaaa
     8701 tccagtatta agagcttgtt tttctgcatt gtacaacgca aaatagtttg gaatagcgat
     8761 attttttgga cagccttcta cacaatactg acaggcagta caaggaacta aaattgattc
     8821 gttgataatc tctactgctt catctataat attatattcc tcttgtacga atggtttgaa
     8881 attttgcata tatcctgtat tgtctaataa ttgttccata tttgacatcc cacttaagac
     8941 catcataaca ttatctttac ttgctgcaaa acgtattgcc caagatgaaa ttgacatatc
     9001 tggattgtag tcattcaata atttctctgc tttttctggt atctttgcaa gtgtacctcc
     9061 ttttactggc tccataacaa ttacaggctt attatgcttc tctgccacct cgtagcattt
     9121 tctagattgt atgctttcat tatcccaatc caaataatta atctgtaatt gcacgaagtc
     9181 tacttctgga tgctcactca agactttatc caataattct gcactatcat gaaaagaaaa
     9241 accaatattc tttatttttc cttctttttt cttttcttca ataaacttaa agctatcaaa
     9301 ttttttagct acttcataat gtgacacgcc tatattgtgt aaaagatagt agtcaaaata
     9361 gtctacacca catttttcta attgctcatt aaaaatggtt tcttgctctt cttttttctt
     9421 taaagccatt aatggaagtt ttgtagctat agtaaaactc tctcttttgt gtctttttac
     9481 tagagattct ctaagtgcta tctcactttt tcccatgtga tacacatagg ctgtatcaaa
     9541 atatgtaaat cccctttcta aaaaagtgtc taccatttta tttaaatgtt ccatatcaat
     9601 agttgtaaca tcattttcat cttttattgg taaacgcatt aatccaaatc ctaatttttt
     9661 tgcctccatt gttttacttc cccctttaaa acttgtttgt tttttctctc tactttaact
     9721 attcttacaa gtatataatt cttgtataat tatactttac tacttggagt taacttcaag
     9781 tcaatagatt tttttatttt taagacaaat attataaaaa tacagttctt taatattaat
     9841 ccattacatg tttctttcac cctccaattc tcaaacttca caaactccaa catgtaaaga
     9901 aatatatact ttaagaaatt cagtccatta atattactac aaatactttt tagatatgtt
     9961 taaaaagaaa tttcacctaa aatgtttata aaagatttag aaataggtct tgtgtctatt
    10021 ttaaaatata taaaacagtg gtaaagactt atttataaga taaaaaatga atagtgtata
    10081 ccctttttaa aaagtttttt cctttttata tttgtgatac aatgttaaat ataaaaagga
    10141 aaattttgta ttaaagaaat cttatttgtt gattttgtaa cttcaaactc agcatcaatt
    10201 tgattttaag attatatttt attaagtatg ctaaataaca aatttttaaa gattgtgagg
    10261 taaaaatatg aataagaaaa atttagctat attaatatca ggtatgatgt ttttttctag
    10321 ttcttcagca atttttgcag acaatactac aaagcaagaa agattaatag gtaaaacaag
    10381 atatgaaaca gcagtagaag taagtaaatt gggatgggtt caatcaaaaa cagccattat
    10441 agttaatggt aattctattc aaagtgcact ttgtgcaaat ccatttgcta agctaaagaa
    10501 tgcacctata cttttagtaa acaacaacag tattgaaaat tcaactaagg ctgagcttaa
    10561 aaggcttggt gtagataatg tgtacattgt agatagtgga aatagcattt cttccaaagt
    10621 agaaaatgaa ataaaatcac ttaacataaa aataaataaa atagtaggaa acaatatata
    10681 tgaaatgtct acaaatgtac ttaaagaaat agataagata aaaaaggtag aaaatgttgc
    10741 agttgtaaat ggactaaagg gactatctga tgcagttagt atagcttctc cagcagcaat
    10801 acataatatg cctataatat tagtatcaga tgaaaacagc aatgatagta gtataaattt
    10861 cttaaacaat aggtctataa aaaaatctta catagttgga ggaaataaga ttttatcaga
    10921 caatacagta aagaaatatc caaacagaga aagaatcagt ggttcagata gaaacaatac
    10981 taatgcaaaa attattgata aattttataa agataaaaaa ataaataatg tatttgtagc
    11041 taaagatggt attaaccaag aaaatgcatt agtagattca ttatcaatag gagtactagc
    11101 tgctaaagaa aattctccta taatgatatc taatggtagc cttgtaaata gtcaaaaaga
    11161 atttattaaa aataaagata tagatgaaat aacacaagta ggaggaggat ataatcaaaa
    11221 agcatttgaa gatattttaa aactaaaaaa tgtgaacata agaaataaaa ctaaaaaaat
    11281 atctttaata caggctaaag aaatattaaa acaagctctt ccaggaagca atattttagt
    11341 ttgttattat gatgaagatg ataatgaata tgactgcaaa gttataaagg ataatatcct
    11401 atatgagata gaaattgatg cctactctgg taaaataata gaaattgaaa aagatgatga
    11461 cttaaatgat aataatttag gtacacaaaa tatatcattg gataaagcta agtctatagc
    11521 attagatgct gttcctaatg gtaatttaat ctattgtaaa tatgatgctg aagataacga
    11581 atacgaagct aaagtaataa aaaataagaa aacatatgaa ttagagataa gtgcatttga
    11641 tggaagaatt gtagatattg acatggacaa tgattctgat gatgactttg atgataatga
    11701 taagaataac tctaatacta atgacaacag ttctattatt agtgctgaaa aagctaaatc
    11761 aataatgcta gataaagtgc ctggtgggaa aatagtaaaa tttaaatatg atgaagatga
    11821 caaagaatat gaaggtgaaa taataaaagg aaaaactgaa tatgagataa caattaatgc
    11881 aattactggt aaaatattag aatttgaaat agatgattaa taaaaaatat atattctatt
    11941 aattattaca agcaataaag ccataaatat tgaaattttc aatatttatg gctttatttt
    12001 atttttttta atttaaatcc acattatgcc attatatata tctatgaact cttttaaatt
    12061 aaaatcttgt ccatcatccc aaacctaata aagttagatt ttaaacaata tattgtgata
    12121 taagtaattt gtgtagaatt attgcgtgat ttactcatga agaaacaata aggttaatat
    12181 ataatttgac tatattaaga caaaaggaga catatacaat gaatacaata ttaaaagtaa
    12241 ataacataac taaaacttat aataaagggc aacagccgag tttagataag gtatccttta
    12301 atgtatcaga aggtgaattt ataggtataa tgggagcttc tggctctggg aaaacaactc
    12361 tacttaatgt attatctaca attgataaac caacaagtgg agatattttc ataaataata
    12421 taaatttgaa atcgcttaat gacacttgtg ctgctgattt tcgtaaggat aatttaggct
    12481 ttatctttca agagtatttt ttactagata gtcttacaat tttcgagaat atttcagtac
    12541 ctcttacact tcagaaactc aatccagata aaatagaaaa catggtaaag agtctagctg
    12601 aacgatttgg catagcttct caattaaata aatacccaaa tgaattatct ggtgggcaaa
    12661 gacagagagc ttcagcagtt agagcaatag taaaacatcc tagtattctc tttgcagatg
    12721 aaccaacagg agctttggat tctaattcag ctactgaact actccactca ctacaggaag
    12781 caaatgagga gttgaaaaca acaattttaa tggtaacaca tgatgcctat gcagcaagtt
    12841 ttgcaaatag aattttgatt tttaaagatg gtcatatcat taaagaatta aataaacaac
    12901 agaataatag acatacattt tatgagttga ttattcaaga aattgcaaaa ttggatacta
    12961 aaagatagga ggataattta atgaacattt tttatacggc aataaagaat cttaaaaaaa
    13021 acttttcatt ctattctcta tatctacttt cagtctcttt tgttattact gtatttttcg
    13081 catttacttc attttctgtt aataagataa tgctagaaaa aatttcaact gatggtagag
    13141 ttgaatctat gtgtaatgtt atttcgatat ttttgatggc atttgtcatc ttctatatgg
    13201 catactcaaa tcgattcttc ttgagacgtc gtacaaaaga attaggtata tatgctttac
    13261 ttggctatcg taagtttaca atattatcgc tacttacctt tgaaaatata ttaatttgta
    13321 gtggagcttt tattattgga ttggcattag gagcattggc acataagggt attgtctttg
    13381 gaattacagc actacttaat ttatcaattg ataattcaca gataccattt tttaatataa
    13441 atgcaataaa taaaacaatt atctttatag cattagttgt tttagtctta gctgtatcaa
    13501 atggtaaatt tctttataag acttcacttg taaaattgat aagatatgaa aaaagtgcag
    13561 aaaaaaaatt aaagtgcaga aaaattccag cctttatggg atttattatg attttaagtg
    13621 ggtattgttt atcactagat attcttcgtg gagctaaatc agtatgggta agtattggat
    13681 tttatcaaat gggcttgata actatgatta tcattgtaat tggtacagtt ctctttataa
    13741 atttctttct tccatatatt atgcaaaaga gtaaaaaaaa taaacgtact ttctacacag
    13801 atatgaagat aatagctatt ccaaacttta tctatcgtat acgttcaaac tctaaaactc
    13861 taattatgct tacattactc tctgctacta ctttgacagt ttcaagtgtt atggcactgt
    13921 ccttatacta ccctattgca tctgtttcgc gtattgcacc ttcagagata gaatttagag
    13981 tagaaaaaaa taaacaaata gaagatgtaa aacaaattgt cagtcaatat acgtctaata
    14041 cagatgatat tatttttaca cagacaaata tttataaggt aaaatcttca tcaaaagacc
    14101 ttccatttga atatgaaatt ggtacatcta agaataattt atctaatagt agttcttcta
    14161 aagaagttat ttcaagagaa tcaggatttg aatgtatatc tttttctgat tattatactt
    14221 tattaaaggc acaaaatcga acaaagctac taaaacaact taccccttta agggataatg
    14281 aatgtatttt aatgaaatat caaccaaata atgatggata tagtgaaaat ggaacgattt
    14341 atccattact tgttaatggt aaagaaacac ctgtagctgt aagggaaaca tcattaaaca
    14401 atgctatttc ttttgccaat agtgttggaa cacttatcgt taatgatagt atatatgagc
    14461 aaatagcaca aaataaatta ccaatgacaa gcattttaag tattaatggt aaatcaatta
    14521 aaaataatga aaatttatat agtaatattt cagattattt agatggaaac ccttatttac
    14581 aaggatattc tcacaggata aatgatttat tatcaattaa tagttctaca tttctgttaa
    14641 tcggatttct tgtggtatta ttctttattg ctgttggaag tattttatac tttaacaata
    14701 tatcagcaat ttcagattca acatctgatt ataagatttt acagaaaatg ggttatgata
    14761 atatacaaat taaaaaaatt attaaaaaac aagtattgcc attttttaat attccgtttt
    14821 tacttggtat attggactgt atatttgcta ccttggttta taagactgct ttgatgcaga
    14881 atttacttgg aaattcattt tcacaataca tacctgtgat tattgcaatt ttcttaactt
    14941 ctgtagtgta tttgatatat tatatactta ctattaaatc ctgttgtaaa attgtattaa
    15001 aaaattaata tttaccaaat cgtcaagctt agaggtgtga cgatttggta tacttcattt
    15061 ataagtattt gatataattt aaaattatag attcttaggt ttgatttagt cataaaatta
    15121 atataattaa ctcataagga ggtgtataag attgacaaat tttaatttaa aaatactatt
    15181 tttaggaaac tacattctat cacttctctt catgggaata tctacaagca ctgaaactta
    15241 tatgaatatc caaagcatta ttcccttaat aatttcaaca ttattactca tagacatcac
    15301 atatataacc tcttgtaatg tccgagaaaa taacattatt tcattgtttt gtggattact
    15361 agcactagac agttggtatt tactcttatc atttgtaacc acaccaatag gcactattat
    15421 ttttaaaata ttaagcacag ttatagttta tgtctctatt aaatttatat ttttattttt
    15481 gtttcaaggt agtggataca atttccgtaa gataacagac attttattgc ttatcacctg
    15541 cattggtgca attgtaggag catttatttc taacagaacg tatgcttgta tgtacggaat
    15601 acagtttgtt gttagtataa taagcttttt ctatattatt ttttatcatt tgaaaagggt
    15661 tgtttttgtc ataaaaagcg aaaaaaaacc tatatcgatt tcagtattta taacaattat
    15721 aggattcatt acttattatt tttttacact aagaatatca aatcatattg gtaattttgg
    15781 aatctatcta actgtattgg tattttttat gagtattcat ggtattgcac ttaaagaaaa
    15841 tgatagtttt cctatttcaa cagtttttaa cataaaacaa agggctatat ttctttttat
    15901 aagtatttta gtaattagct tggtcgtact atttgccaat gggaattttt ctataatatt
    15961 aatagctata aatgttttat ttatgcttat tttcctctgt aacattattt tggaatttaa
    16021 ccttagagat aaaaaaaata aagtagttaa aaatagcaag tatacttttg cattagaaaa
    16081 attacaacaa gaagaacaac taaaaacaga gttttctaat tttttacatg atgacatatt
    16141 acagaacctg ctttcaataa aaaacatgat gagtaagtca tatcgcccag aagttcaaga
    16201 tattatatat gaaacactta acaatctaaa tacatgtata aggaagcaaa tgcaagacta
    16261 tcatcctgtt attttaaaaa atttaacagt aaaagaaaat tatcagaatt taattgaaag
    16321 tgtttcttca tcctttccac aaaaaaacat ttgtgtttca tttaagtgtt ctgatacttt
    16381 attcttagtt gaaccatatg atatattgat atatcgactg attaaagaat tattgacaaa
    16441 tgtgtataaa cattcaaatg gtagccatgc atggatagct ttagcactag aaaaaaatat
    16501 aataaaatta aatattaatg ataatggtgt gaaaaataca tcttttaaaa cacctttaga
    16561 tataactaaa attacttttg atacaataaa acacaaaggg attatatcaa ttaaagaaca
    16621 agtagaaaat ctaggaggaa cgataagtgt ttctgataat atacctcatg gagtttgtgt
    16681 acagattaca atccctatga aaggagatgg ttcttataaa tattttgtta gttgatgacc
    16741 atatgctttt tgctaaaagc ttgtcaattg cactagaaga atacaaagaa atcgaacagt
    16801 tttattccac acaggacatc cataatcttt cagaaatgat tcaagataaa aaaatagaca
    16861 ttgtcttaat ggatataaac cttagaggct taagtgaatg tgatggctta aaagtagctt
    16921 ataatttatt agagactaat tcaactatta aaataataat cttgactgga tacgacctcc
    16981 cagtttataa acatgaggct aaaaaaatag gtatctatgg attcctcaat aagaatatta
    17041 gccctgatag tttaattgat tcattattaa aagtctatca agggggaact tgttttccat
    17101 ctgaaaatac tgaagtaatt attgaagatt taactaatat ggagaaaaga attttacagc
    17161 ttatctgtag tggtaaaaaa agaaaaataa ttgctgatga attgtatata agtgaaagaa
    17221 cattatcaaa tcatttacaa cacatatatg acaagctaga tgtttcatca tcagttgagg
    17281 ctattacaaa agctattaaa atgggttata tttctccagt atattaaaaa aagtgagtgt
    17341 ctcaaaataa atcttctaac tcataaagca ctctcctttg tattaaatca aatatatttt
    17401 catcatttaa acaattaaga agaggcttct ctctattttg agacaactcc attttaatac
    17461 actatttata cataaacata ttttaaacaa ctaaaaagca actaatatct caatacatat
    17521 cttcataaca catatcacca taattacaag cagataaaga actctcctca tttaaatacg
    17581 actcaaattc atacccaaac agaattgttg gttctatata tatttccata tctgtatatc
    17641 tccatcttct tacctttgcc tctataacct tgtaaaaatc ttctaaacta tacccttctt
    17701 ttaatctttt atctataagt gccttagtta tactcaaatc agctttaaaa ttttttcctg
    17761 attcattatt taattttctt ataataattt catgtatgct ttctggattt ttataagtag
    17821 aatcttcagt caaatcttca tccgaatttt caaaggtatt tttatttaaa ttctttttat
    17881 ttaaattatt ttttaataat tcttttttct ttgtctcatt ttctgtttca tacccagtat
    17941 gaattataaa atcataatct ttgtctatac tctcttgatt tatacggctg tatgtgttgg
    18001 tattactgtt cttaaacaac tcatttctat gtttatattt cattgttttt tggttattgt
    18061 taatagttgc atcaatgtat ttctctatat ttttgaaaat atcaccattc tttgttacaa
    18121 tttctgcatt actactttca gaacaaacat aactatatac agaacaacaa tttcccttta
    18181 ctcccctact tattaatctc aatataccta aatctataaa ttcatttaca agtctactta
    18241 ttgtagatat agatacatca aggtcagatg cagctttcct tactgtcatc ataaattgtc
    18301 ctcttggcag agttcctttt aatctttgta tattttcttt tctcattaca tagtgattaa
    18361 accttattct atttacatct cttttttcat taaaatataa tactgctgaa tttaattgat
    18421 aaaaactatt gctcatattc ccactcctcc aatttaaata tataatttgt tgtaaagttt
    18481 ttagattttt aaataactta ttaaatgatt atttttatta aaaagtattc tttgatattt
    18541 tcattttaaa gttttaagta taataagtta atagtgtaga tttaactttt ttatttaaac
    18601 aataaactta atattatttt taatataaaa attttattaa atttagtatg taatttttta
    18661 atcttatttt attccatcta tactcttatg ctaagtaata actttgataa tatttcccta
    18721 acttatgttt caactggttt atattgcaat ttatttaatt acagcttatt tgcataataa
    18781 ttcgtccata
//
LOCUS       DBGJFF010000072        18786 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.073, whole genome shotgun sequence.
ACCESSION   DBGJFF010000072 DBGJFF010000000
VERSION     DBGJFF010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18786
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.073"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(80..616)
                     /locus_tag="ACOIAF_002460"
     CDS             complement(80..616)
                     /locus_tag="ACOIAF_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HGZ2669467.1"
                     /translation="MEYKSPVYGIIAVPIEKIESNDYNPNAVAPPEMQLLYKSIKEDG
                     YTMPIVCYYNEEKDKYIIVDGFHRYRVMLENEDIYDRENGLLPVSVIDKSLDERMAST
                     IRHNRARGSHNVDLMSKIITELCEIGKSDEWISKHLGMEVDEILRLKQITGLAYLFKD
                     KEFGNAWIPFGSDIEEEN"
     gene            complement(634..1947)
                     /locus_tag="ACOIAF_002461"
     CDS             complement(634..1947)
                     /locus_tag="ACOIAF_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HGZ2669468.1"
                     /translation="MDKRYLESDVFEAFQERLSYLFNEFDYIYVSFSGGKDSGLLLNL
                     VLDYKRKNNISSKIGVFHQDFEAQYSLTTEYVEKVFEDNIEDIEPYWVCLPMATRTAV
                     GNYEMYWYPWDDEKEELWVREMPVKEYIINQKNNPFTYYKYKMRQESLAKQFGRWIRD
                     IHEGAKVVCLLGTRAAESLQRYCSIVNKQHGYKGKCWITKEFKNVWTASPIYDWEIED
                     IWLAHYKFDYDYNKIYDLFYKAGVAPRKMRVASPFNDSAKESLNLYRILDPDVWVKLV
                     ARVQGVNFTSIYGKTKAMGYRSVKLPYGHTWKSFTYFLLDTLPENIRNSYIKKFSTSI
                     EFWQNTGGGLSEIVIRELLRKGYKIELNGVSNHTKDKKSRVIFLDDIPDDTDDIKFTK
                     DIPSWKRMCYCILKNDHMCRFMGFGPTKEQRIRTEYIKKKYEKIH"
     gene            2302..2742
                     /locus_tag="ACOIAF_002462"
     CDS             2302..2742
                     /locus_tag="ACOIAF_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HGZ2669469.1"
                     /translation="MYERMLDKSNKPCLSQIIEYIGKDGYARLCKLETYLKTQYNLSR
                     ELRFPFGNNYGWGYKYSHKSFHLFYAFFEKGAFTVLIQIGDKQVPAVEKTLVALTPKA
                     KELWVTRYPCGNNGGWIKYRILEDADLLDIIQYIRAKKSPPKQN"
     gene            complement(2926..3114)
                     /locus_tag="ACOIAF_002463"
     CDS             complement(2926..3114)
                     /locus_tag="ACOIAF_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HGZ2669470.1"
                     /translation="MNKNNNLVIICMFIGMILGMAIGCAIGISKGNVGITMCYGLIFG
                     MIIGICIGTIIKNSNKKE"
     gene            4136..4531
                     /locus_tag="ACOIAF_002464"
     CDS             4136..4531
                     /locus_tag="ACOIAF_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6110 family protein"
                     /protein_id="HGZ2669471.1"
                     /translation="MNILKMKKTGIFLGGVLFGTVGVKILSSDCVKKTCTRATAGALK
                     VKDSVLETTTKIQENVEDILAEAKEINEQEDLSTSKVNVVQDTLKCEQEVLKTETIKT
                     VEDTNSNSIKCEEIKDNVIEEKNSTCTTE"
     gene            4574..6673
                     /locus_tag="ACOIAF_002465"
     CDS             4574..6673
                     /locus_tag="ACOIAF_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HGZ2669472.1"
                     /translation="MNFYIKHEIRGRIRFDLQLGKLNNKQSDTLLYYLTSINGITKAK
                     VYDRTGDAVIYYTGEREYIIREITKFSFNNSKIENLVPEHTSRELNNYYRDKLANKLL
                     VKSISKIFFPKSLRFFLIGLHSIKYFKKGLSSLLNKKIEVSVLDATAIGISIFRKDIN
                     TAGSVMFLLGIGELLEEWTRKKSIDDLAQSMSLNIEKVWLRKGDTEILIPISEIKEGD
                     LVSVTMGNMIPLDGVIVSGETMVNQASLTGESLAVNKKEGSYIYAGTVIEQGNIVMCV
                     KEKAGTTRFQKIVTMIEESEKLKSSVESKFEHLADTLVPYSFLGSILTYAITRNPIKS
                     LSILMVDFSCALKLSIPISVLSAMRECNNNNITVKGGKFLEGVACADTIVFDKTGTLT
                     KAQPTVSDIITFQNYNKEDMLRLAACLEEHFPHSIANAVVYEAEKQGLSHKEMHTEVE
                     YIIAHGISTKVDDKKVVIGSKHFVFEDEKCTIPDGEEDKYNNLSDEYSHLFMAISGKL
                     SAVICINDPLRKEAKYVISNLRECGIKKIVMMTGDSEKTAKSIASKVGVDEYYSEVLP
                     EDKANFVKSEKLKGRKVIMIGDGINDSPAISESDVGIAMSEGAEIAREISDITISADN
                     LNNLIILKQISNKLMKRVDLSSKFIIGFNLGLILLGVGGFVRPSTSAFLHNASTVGIS
                     LNSMTNLLENTNTYNYH"
     gene            complement(6898..12108)
                     /locus_tag="ACOIAF_002466"
     CDS             complement(6898..12108)
                     /locus_tag="ACOIAF_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HGZ2669473.1"
                     /translation="MANKLYSEIVNLLEEGRDELRKYDLKEKSILLFLGASGVGKSTC
                     INYLKGCVMEEKTDEETGQIYITAKDSAVEIGNGVYSKTLCPEVVDIANRDFSLCDCP
                     GFFDNRGAEYMIAGAMLVRETISTSSKVKGMVVILDYQSLLDSRHTLLIETGKNLKDM
                     LGDYAKYKENFFFLINKIPKTLIGNVTEEKIRNIFEKAKEDLDSQEKDYDGCKELVYL
                     LNVALSDGVSIKLCNPLDKQPEKLLNEMKNLPETFEKTDLGLMLTASCKIEIQKAVDV
                     TYKDLEKLLVPYIESVKKGMEDSISILSSINSLKDKKIQLDKMYTDLCSRTNNMKELM
                     EFVQELSYGDGVQKVNILEETRLLLELNSYCSETHIVGLSELLKSLLKNTLEDTLKDI
                     KYWIAEEEYREEEAKRLEDAEYRIEKIQSCTYIKSYEKENVLSIYIPIPKVNLSDLTK
                     IISGYQLNILSSIYMYGDCELKIDCNMEKEVFSGKNIVLLTEKLHIPEGTYSINVSGK
                     DAEKLVGESSIYDGMNGRNGNNAGTSGDIILSFKEIEKRGELKLYVDGGRGGNGQDGK
                     KGDTGITGEAGANAKIGSKKESKEFANVKGTKYYVVKAEKGKQGGTGGTGGNGGNAGL
                     GSKSGQVYLLGKAPLLKSAISKKDGNHGLAGKGGSGGAGGAGGLGGIRGIYEETIRYI
                     GTSPPMDNSDRNYYYFDRYVFNHALNQKQAADDGEKLNALYEEHKDWTISKREDSGSA
                     GAEGKAGHSGDALKEKELLKQEYSLSIDTFFYEFFERFGFSGIKGYRNLRLLCAVGIL
                     EADILEFSHIYEIFYAVEKIEMQLNREILQKYYSFEKKNKAVLSLDDMNTKKEFYKEA
                     KEIYEYLINKLMQSDRIESFVGKNQNFSEWSMILFALEQKTAFYVQMIEESEIGGNRV
                     TDIRSLTDYLDKTLRELEQGKKDELKEGYFNTYKESILTKIESGNQQIEVLKNELENK
                     MLSVNAKMNLLIQEIIKMKEDEKEDIEELKKKQAKLEEQMKTQLILSGIQSALSLLTA
                     FSGVGQACLGAFDSAKSLKENLSAKEEEYKWTNRMVNFSDFKLDSDGMRFVLGEQLSE
                     DRKNALDKMTYNLAGVDEERRTKFDQIKEIIVKSIDDDNYADKADDIQRAKEGLSESE
                     YKEIYTKIQKKEIGMLEANYNNKQQKDKLAALEKSKQAIERQQKTSKIKNALSIGSSA
                     CSLINTAISGVNNCLNVKSSFESKIEVVKEAIKDKENNIKKLDELNTQMVEFKDKTLK
                     ESVIPFVEKFDSTVKNQDIFENEISKLKMKEVLTELSIKFGSLKMENSEEISIIFKKV
                     QDMMETQIIITDKIDKKKDNLAMGELIYQMSDSGNQMNEYQKKLIHQIELNQVRYLCQ
                     VEAAAFQLWTFPFGSNIVNILNRCHVGITEDENEVINQAKEQNRQIREFLDQDAYQWQ
                     KTDNHILKVALTGSLDGKSRVGFENTYSLYDCNEEEVKKLLTGDEISFEVPLNKNFDA
                     IKYVTMYANIPNLRRNILEDSLKVKVHIHLYDSGYFSLYDDKKDVYYQYVFPQNYINI
                     THGLDIKYNDSDSINSIWSSVEDKGIEKFKNAGLNSGRSPYSMAKISLKAHFNRKLSY
                     DSLTNEQKKKLESYFDKPSKYAWSDSIDILRSLGYIDLKGILSKSWYEIKKDIDSDGE
                     IMKHFSILQVDLLERIIYEQTSILKENIPNLTIELVGCGIYVESEFNYLKQNDLKCYE
                     ERLVENTIIG"
     gene            complement(12123..14618)
                     /locus_tag="ACOIAF_002467"
     CDS             complement(12123..14618)
                     /locus_tag="ACOIAF_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGZ2669474.1"
                     /translation="MPENNNQLLEEITLLLNEGKVAINKAENQNLILILGGTGCGKST
                     AINYLAGCVMMEVEDEDTGISYIDCLDPVAEIGSGAVSQTLYPNIVNMRKPIEGMDAK
                     LCDTAGFSDNRGVAHDISAAVSLGEVFKNSASICAVIVLIPEADILDSRLTKVLDLFQ
                     QLNMCLHKENFKGSITFFISKSFLGRTEKQIYNVIKRKYEQLCENLDSNIEEKAWLFE
                     ELLKDNGKNIHLCKPLASDEREGLLKQVLELDEVKDKTSAFQYPISADARLVLDTLIK
                     EIQNNIVSSLEDYVKAYTEDFINIIGSITMIQEIAKYDEVVKVFKNWLESTTSLTIES
                     FINVLAKQSGLFLQDSCMKLQNKIKGNLDIIDALSKYTEHGTGEILLPSVHMEFIIEK
                     ASKKIMEQNFIIIRKNLEKVLVSYEVQKQVFTLPNREKLMEVANKKDYVYSVSDFNSI
                     LTNVTVTQNEELEDSITRLSAYGEEGNEIIREFIGKYILNPFIVKTININDKKGIEIR
                     ALIPNLVISSVLEYFKNRKVDDNVSTFMFFAKDTIYMDDNLGMDFASEKNIILASNNL
                     DVGSNVKINVSGKSGELVDNSMYGKNGEDGRDAGNIYLYIKEEIRNYRLTLIANGGNG
                     SKGIDGKNGETGASGSNGADAGYTSYFHGQMFKGYFEKGTSGKKGEKGGAGGNAGRGG
                     NAGKKGNIYFISDSEEQKNRIINHCDITVKDGEAGPNGRPGLGGYGGSGGYHGYNTLS
                     FSPSGIDKTVYYTGYFTSWDYKIEKVVYYRVELSGGSWNEGKTGPAREYAEYGDKGDN
                     GGFNAPNTKLSTAVSKVEENLIKEKYQGYCSEI"
     gene            complement(15287..15574)
                     /locus_tag="ACOIAF_002468"
     CDS             complement(15287..15574)
                     /locus_tag="ACOIAF_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669475.1"
                     /translation="MRLNITKQNTIEVENSRKEDVYFVIVDNVFFERQGDVFNSITNN
                     NLNNYDVKILPTDNIFLELKITSTKDLEPVFIVVEDRQYNHGSIMFKPVLV"
     gene            complement(15594..15869)
                     /locus_tag="ACOIAF_002469"
     CDS             complement(15594..15869)
                     /locus_tag="ACOIAF_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669476.1"
                     /translation="MSLFVGGWSKFRAVEEEDLKVFNEAVGMLEGVGYEPLIVSTQVV
                     AGTNFKFICNATSVTNPPQNYLAEIVVFKPLPCDEHNATITSINIIK"
     gene            complement(16504..16683)
                     /locus_tag="ACOIAF_002470"
     CDS             complement(16504..16683)
                     /locus_tag="ACOIAF_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6440 family protein"
                     /protein_id="HGZ2669477.1"
                     /translation="MGKDRFEKVYSQGKINATEIWVDKETGVNYVFHYYGHAAGFTPL
                     LDREGKPVISPIVSK"
     gene            complement(16968..18443)
                     /locus_tag="ACOIAF_002471"
     CDS             complement(16968..18443)
                     /locus_tag="ACOIAF_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGZ2669478.1"
                     /translation="MKRKPQYLIIFEHIIQQIYTGKITIGDRLDSIKSMSEEYMVSKN
                     TIKTVIKMLSEKGFIETKAGSRPVLIQSTSKQSIESDLLYEKILQISEIYKIFSLIFP
                     SIAMYNIKRFTSKDFEELNEIIDCMEKNSSNYLVFKEQRLKYINKLISKTNNFLIHHF
                     CEIIQHDVIISQLAYNFDNLDNLDNCCIDFFRNKYIEQVRKTYLYGLDGNLIEFKKLL
                     TYMYKACRESILKMVENRAPQSIDTSSFNSVTLEKCYLYDLIVSDVICQIFEGNIKIG
                     DCLPSITSIINRYNVSLPTVRSAYNELNELGIAKTINGKGTYVTLFSEYDDDYINTEE
                     GAKRLSLLLDAMEFVTITLEDVVLLLGNKMDLIAVDKIETHLRYLQNNCEECKVYPDF
                     VLLCKIVDYTQIYVLQETFLHLKQYMIFGIYLERFFHEEYNEILKTRFDVCFEILRYL
                     KKGDVERFAESFSNIFKESLQYLKKVISSLNVDMRLKLGIL"
BASE COUNT         6240 a         3291 c         2054 g         7201 t
ORIGIN      
        1 taatattaaa actctttatt ttttaatata tattttaaat tttctttttt aataaatctt
       61 tttcttttaa taaatctatt taattttctt cttctatatc agagccaaag ggaatccatg
      121 cattaccaaa ctctttatct ttaaataaat aagctaatcc agttatttgc ttcaatctaa
      181 gaatttcatc aacttccata cccaggtgct ttgatatcca ttcatcagat tttccaattt
      241 cacaaagttc agttataatt ttactcatta aatccacatt atgagaacct cttgcacgat
      301 tgtgtcgaat agtacttgcc attctttcat caagagattt atcaattact gaaacaggaa
      361 gaagtccatt ttctctatca tatatatcct cattttcaag cattactcta tatctatgaa
      421 aaccatctac aataatatac ttatcctttt cctcattata ataacagaca attggcattg
      481 tatacccatc ttcttttata cttttataca acaactgcat ttctggtggg gctactgcat
      541 ttggattata gtcgttagat tctatctttt cgattggcac tgcgatgatt ccatatacag
      601 gacttttata ttccatagca tcttaactcc tatctaatga attttttcat atttcttctt
      661 aatatattcc gttctaattc tctgttcttt tgtaggacca aatcccataa atctacacat
      721 atggtcattt tttaaaatac aataacacat tcttttccaa cttggtatat ctttggtaaa
      781 tttaatatca tccgtatcat caggtatatc atccaaaaat atgactctac tctttttatc
      841 ttttgtatga tttgaaactc catttaattc tattttataa ccttttctca acaattcacg
      901 tataactatt tctgacaaac caccacctgt attctgccaa aactcaatag atgttgaaaa
      961 ttttttaata tagctattcc ttatattttc aggtaaggta tcaagcaaaa aatatgtgaa
     1021 acttttccat gtgtgtccat atggcaattt aacactacga tatcccatag cttttgtttt
     1081 gccataaata gaagtaaaat taactccttg tactctagca accaatttca cccatacatc
     1141 tgggtccaat attctgtata aatttaaact ctcttttgct gaatcattaa aaggtgatgc
     1201 cactctcatt tttcttggag ctactccagc tttatagaat aagtcataaa ttttattata
     1261 gtcatagtca aatttatagt gtgctaacca tatatcttct atttcccaat catatatagg
     1321 agatgccgtc cagacatttt taaattcctt agttatccag cactttcctt tatatccatg
     1381 ctgtttattc acaatagaac aataacgttg tagagattct gctgctcgtg ttcctaataa
     1441 gcaaacaact tttgcacctt catgtatatc tctaatccat ctaccaaatt gttttgccaa
     1501 actttcttgt ctcattttat atttataata agtaaaagga ttattttttt gatttattat
     1561 gtattctttg actggcattt cccttaccca taactcttct ttctcatcat cccatggata
     1621 ccaatacatt tcataatttc ctacagcagt tcttgtagcc attggtaaac acacccaata
     1681 tggttcaata tcttcaatat tatcttcaaa tactttttca acatattcag tagtcaaact
     1741 atattgagcc tcaaaatctt gatgaaatac tcctattttg gaacttatat tattctttct
     1801 cttataatcc aaaactaaat ttagcagtaa accactatct tttcctccgc taaaagatac
     1861 atagatataa tcaaattcat taaaaagata agaaagtctt tcttgaaaag cttcaaatac
     1921 atcggattct aaatatctct tatccatttg aattatcccc tttcaattag aagtttataa
     1981 aaccttacaa tgcaatatta ttatgctttt ttagcagtaa tcagcataaa ttatacatta
     2041 tttaaataca caaatccaac taactacata taattataat gtaattatac ttttgtatta
     2101 taaactatat tttatcatta ttttacattt ttttccaata tatataaata tcatattttt
     2161 ctattagcta aaactaatta ctttatattt ctacgtatta gtataataat acataaaaac
     2221 ttgaataatg cataaaaact tgacatactg ttgtcataat tactttgcta taattaaaag
     2281 taaaaatgga ggtaaattct aatgtatgaa cgtatgcttg ataaaagcaa taaaccctgt
     2341 ttgtcccaaa taatagaata tataggtaaa gatggatatg cacgattgtg taagttggaa
     2401 acttacttga aaactcaata taatttatca agagaacttc gatttccttt tggaaacaat
     2461 tatggttggg gttacaaata tagccataaa tcttttcatt tattttatgc tttttttgaa
     2521 aaaggtgcat ttactgttct gattcagata ggtgacaagc aagttccagc agtagaaaaa
     2581 acgttggtag ctttgacacc aaaagctaaa gaactctggg tcactcgtta tccttgtggc
     2641 aataatggag gctggattaa gtatcgtatt ttagaagatg cagacttact tgatattatt
     2701 caatatataa gagcaaaaaa atctccacct aaacaaaatt aacttttatt ttttgtgatt
     2761 taaatatagt gtatataaat tccattcttt ccatattaaa aaataatata aaaaatatct
     2821 attagtataa cttatttagt atgtcttatt ctacttttta cagataatta aaaagtttta
     2881 tcataaataa atcataacta gacatcctag taaatcaaaa ttaatttatt cttttttatt
     2941 cgaatttttg ataatagtcc ctatacatat gccaattatc attccaaaaa ttagaccata
     3001 acacattgta atacctacat ttcctttaga aatacctata gcacaaccta tagccattcc
     3061 aagtatcatg cctataaaca tacatataat aactaaattg ttgtttttat tcatagtttt
     3121 tctccattca atatatttta aattgcctag ctcgccttct tagcttaaaa caaagaaata
     3181 taaattaact ttctttcatt ctaatatctg cctgtataaa tgtcaaacaa cacatttctt
     3241 gtcataacct cctctttttg aagtaagtac aatctcccag acgcaataat atcaatttcc
     3301 taaaaccatt taagtaactt ttatatttac ataatattta caatcataaa ctatattgtt
     3361 attcatttat acatcataaa cactccctcc taatcattat tatacttaaa ataatgttca
     3421 tttataataa tatatctaac gcactttaat tgaaaaaact aaaactttgc ctttcattcc
     3481 catatataaa taagtatact ttttgatgct ctattacaaa tcaactaaat taattgatat
     3541 aaattatcat tttctgttga aaataccatt cgcacatgct atacttaatt gcataaatgg
     3601 taataaatgt catatgattt tacaaatcta ttaataacaa ttattacttt tactgtattt
     3661 taaagttttt ggactaaata tcaagaactt tctaaaatac aaagtacaaa aaatttatat
     3721 aagttataaa tttatatgaa ctagataagc tcaaacaata tgttatgtat aaactgtgta
     3781 gtattcggtt ataaatttat ataaattata taagctagat aaagtattac ctcaactata
     3841 taagctagat atataaattg cttatataag ctagataaga attatttcaa ctatataaac
     3901 tacatatata aattacatac ataagctaga tatataaact gcatatataa gctatataag
     3961 aattatttca accatataaa ctacacatgt aaaattcata taaatttact aatataaatc
     4021 aaataaaatt actaaactct ctaaactaat tttgtaaaac atactctatt tttaacattt
     4081 gttaccttat acataagtac aaacaaaatc taactaaatg aaaggataat gtaaaatgaa
     4141 tattttaaaa atgaaaaaaa cagggatatt tttaggagga gtactttttg gtactgtagg
     4201 tgttaagata ctttcaagtg attgtgttaa aaaaacttgc actcgtgcaa cagcaggagc
     4261 attgaaagtt aaagattctg ttcttgaaac aactacgaaa attcaagaaa atgtagaaga
     4321 tattttagct gaagcaaaag aaatcaacga gcaagaagac ttatctacat ctaaggtaaa
     4381 cgttgtacaa gatactctta aatgcgaaca agaagttctt aaaactgaaa ctataaaaac
     4441 tgtagaagat actaattcta atagcattaa atgtgaagaa atcaaagata acgtaattga
     4501 agaaaaaaat agtacttgta caacagaata aatttaatga ttattatgat taataatatc
     4561 aggaggaagt aaattgaatt tttatataaa acacgaaata agaggtcgta tacgctttga
     4621 cttacaactt ggcaaattaa acaacaagca gtccgatact ctcttatatt atttaacttc
     4681 tataaatggt attaccaaag caaaagttta cgatagaact ggtgatgcag tcatttatta
     4741 cactggtgaa agagaataca taataagaga aatcactaaa ttcagtttta acaatagcaa
     4801 aatagaaaac ctagttcctg aacataccag cagggaatta aacaattatt atagagacaa
     4861 attagcaaat aaactgctag tcaaaagtat ctccaaaata ttttttccaa agtccttaag
     4921 atttttttta attggacttc attctataaa atattttaaa aaaggtctct cttccctgct
     4981 aaataaaaaa atagaagttt ctgttttaga tgccactgct ataggtattt ctatttttag
     5041 aaaagatatc aatacggctg gttcagttat gtttcttctt ggtatcggtg aactattaga
     5101 agaatggact agaaaaaaat ctatagacga cttggctcaa agtatgtcac taaacattga
     5161 aaaagtatgg ttaagaaaag gtgacactga aatcttaatt ccaatttcag agataaagga
     5221 aggagattta gtaagtgtaa ctatgggaaa tatgattcca ttggatggag ttatagtatc
     5281 aggagaaaca atggtcaatc aggcatcact tacaggtgaa tcccttgcag ttaacaaaaa
     5341 agaaggctct tacatatatg ctggaactgt aatagagcaa ggcaatattg ttatgtgcgt
     5401 caaggaaaaa gctggtacta ctagattcca gaaaatagta actatgattg aggaatctga
     5461 aaaactaaaa tcttctgtag agagtaaatt tgaacatcta gcagatacat tagttccata
     5521 cagcttttta ggaagtatat taacctatgc aattactaga aaccctataa aatcactctc
     5581 tatattaatg gtagatttct cttgtgcatt aaaactctct atccctattt cagtactatc
     5641 agccatgaga gaatgtaaca ataacaacat tactgtaaaa ggtggaaaat ttcttgaagg
     5701 tgttgcatgt gcagatacta ttgtatttga taagacaggc acattaacaa aagctcaacc
     5761 aactgtttca gatataatta cattccaaaa ttataataag gaggatatgt taagacttgc
     5821 agcttgctta gaagaacact tcccacactc catagcaaat gcagtagttt atgaagctga
     5881 aaaacaaggg cttagtcaca aagaaatgca tacagaggta gaatatatta ttgctcatgg
     5941 catttcaaca aaagttgatg ataaaaaggt tgtaataggt agcaaacatt ttgtatttga
     6001 agatgagaag tgtactatcc cagacggtga ggaggataag tacaacaatc tatcagatga
     6061 gtattcacac ctatttatgg caatatcagg taaattatca gcagttattt gcataaatga
     6121 ccctttacga aaagaagcaa aatatgtaat ttcaaattta agagaatgcg gaataaaaaa
     6181 gattgtgatg atgactggag atagcgaaaa aacagctaaa tctatagcaa gtaaagttgg
     6241 tgttgatgaa tattattcag aagtcttacc agaagacaaa gccaattttg ttaaaagtga
     6301 aaaactaaaa ggtcgcaaag taattatgat tggtgatgga atcaatgatt ctcctgctat
     6361 ttcagaatca gatgtcggaa ttgcaatgag tgaaggtgct gaaatagcaa gagagatttc
     6421 agatattacc atatcagcag ataatttgaa taatttaatc attttaaagc agattagtaa
     6481 taagcttatg aaaagagttg atttaagttc taagtttatt attggcttta atttaggtct
     6541 gattttactt ggtgtcggtg gatttgttag accaagtaca tctgcctttt tacacaacgc
     6601 atctactgtc ggaataagtt taaacagtat gacaaattta ttggaaaaca ccaatactta
     6661 taactaccat taatctccat tacataaaac atatgatata aaaaatttat aattcatctt
     6721 ttaaaaagaa tatgtcttaa aattagcttt tttctaatca aaattatata aagcatactt
     6781 tacaaatttt aaactcaact tatttctatt ttttacaaaa aaggagatga ttcaaatttt
     6841 cattttgaaa catccccttt tgtaaatttc tttaaaattt atatattaat tatattttca
     6901 accaataatt gtattttcaa ccaatctttc ttcatagcat tttaaatcat tttgctttag
     6961 ataattaaat tcagactcta cataaatacc acagcctacc aattcaattg tcaaattagg
     7021 tatattttcc tttagtatac tagtctgctc ataaataatt ctttccaata aatcaacttg
     7081 taatatagaa aaatgcttca taatctcccc atcactatct atatctttct tgatttcata
     7141 ccaactcttt gacaaaattc ccttcaaatc aatataccct agactcctca atatatctat
     7201 actatcgctc catgcatact tactaggttt atcaaagtat gattctagtt tcttcttctg
     7261 ctcatttgta agtgaatcat aagaaagctt cctattaaaa tgtgccttca aagaaatctt
     7321 agccatagaa tatggacttc taccagagtt taatcctgca tttttgaact tttcaattcc
     7381 tttatcttct actgaagacc atattgagtt tattgaatct gaatcattgt atttaatatc
     7441 aagaccatgt gtaatattta tatagttttg tggaaataca tattgataat agacatcttt
     7501 tttatcatca tatagagaga agtatccact atcatataaa tgaatatgta cctttacttt
     7561 taaactatcc tctaaaatat ttcgtctaag gttcgggata tttgcataca tggttacata
     7621 ttttatggca tcaaagtttt tatttaatgg aacttcaaaa ctaatttcat ctcctgtcaa
     7681 aagctttttc acttcttctt cattacaatc atatagagaa tatgtatttt caaatcccac
     7741 acgtgatttc ccatctaaac tgcctgttaa tgctaccttc aaaatatggt tatctgtttt
     7801 ttgccattgg taagcatctt ggtcaagaaa ctctctaatc tgtctatttt gctctttcgc
     7861 ctgatttata acctcatttt catcttctgt aatccctaca tggcatctat ttaagatatt
     7921 tactatattg cttccaaatg ggaatgtcca tagttgaaaa gctgctgcct caacctgaca
     7981 caaatatcta acctgattta attcaatttg atggataagt tttttctgat attcattcat
     8041 ctgattacca ctatctgaca tttgataaat caattctccc attgcaagat tatctttttt
     8101 cttatcaatc ttatcagtaa tgataatttg agtttccatc atatcctgta cttttttaaa
     8161 tattatagaa atctcctctg aattttccat ttttaagctt ccaaacttta tgcttaattc
     8221 agttaaaact tccttcatct ttaatttact tatctcattt tcaaaaatat cttgattttt
     8281 aactgtacta tcaaattttt ctacaaatgg tataacagat tctttcaaag ttttatcttt
     8341 aaattccacc atttgagtat tcaattcatc taatttctta atattatttt ctttatcttt
     8401 aattgcctct ttaactacct ctattttact ctcaaaacta gattttacat taagacaatt
     8461 attaacacca ctaatcgcag tatttataag agaacaagca gaacttccaa tactcaatgc
     8521 atttttaatt ttagatgtct tttgctgtct ttcaatagct tgtttacttt tctctagtgc
     8581 agctaactta tctttttgct gtttattatt gtaatttgcc tccaacatac ctatttcttt
     8641 tttctggatt tttgtgtaaa tttccttata ctcgctttca cttaacccct cttttgctct
     8701 ttgaatatcg tcagctttgt ctgcataatt atcatcatct atagacttaa ctataatctc
     8761 cttaatttgg tcaaatttag ttcttctttc ttcatcaaca cctgccaaat tataagtcat
     8821 tttatctaaa gcattttttc tatcttcact tagctgttct ccaagtacaa atctcatacc
     8881 atcagaatcc aatttaaaat ctgaaaaatt aaccatcctg ttagtccatt tgtattcctc
     8941 ttctttagct gaaagatttt ccttcaaaga ttttgcactg tcaaatgccc ctagacaagc
     9001 ttgaccaaca ccactaaatg cagtcagtaa actcaatgcg ctttgtattc cagaaagaat
     9061 taactgtgtt ttcatttgtt cctcaagttt tgcctgtttc ttttttagtt cctctatatc
     9121 ttctttttca tcttctttca tctttataat ttcttgaatc aacaggttca tttttgcatt
     9181 aacagaaagc attttattct caagttcatt ttttaaaacc tctatttgtt gatttccaga
     9241 ttcaattttt gttaggatac tttccttata tgtattgaaa tatccttctt ttaactcatc
     9301 tttcttccct tgttccaatt ctcttaaggt cttatccaga taatctgtca aagaacgaat
     9361 atctgtaaca cgatttcctc caatttcact ttcttcaatc atctgcacat aaaatgctgt
     9421 cttctgctca agtgcaaata aaatcatact ccattcgcta aaattctgat tttttcctac
     9481 aaaagattct attctatcag actgcatcaa tttatttata aggtattcat agatttcctt
     9541 tgcttcttta taaaattctt tctttgtatt catatcatcc agagaaagta ctgctttatt
     9601 tttcttctca aaactatagt acttctgaag tatttctcta tttaattgca tctctatttt
     9661 ctcaactgca taaaaaattt cataaatatg tgaaaattct aaaatatcag cttctaaaat
     9721 tcctactgca cacaacagtc taagatttct ataaccttta attccagaaa aaccaaatct
     9781 ctcgaaaaat tcatagaaga atgtatctat tgacaaagaa tactcctgct ttaacaattc
     9841 cttttctttt agtgcatctc cagagtgacc ggcttttccc tctgctccgg ctgaacctga
     9901 atcttcacgt ttgctaattg tccagtcctt atgttcttca taaagagcat ttaatttctc
     9961 tccatcatct gctgcttgct tctgatttaa agcatgatta aacacatatc tatcaaaata
    10021 atagtagttt ctatcactat tatccattgg aggagaggta cctatatatc ttattgtttc
    10081 ttcatatata cctctaattc caccaagacc tcctgcacca ccagctcctc cagagcctcc
    10141 ttttcctgct aaaccatgat ttccatcttt tttacttata gcacttttta ataaaggagc
    10201 ttttccaagg agataaacct gtccagactt gcttccaaga cctgcatttc caccattacc
    10261 tcctgtgcct ccagttccac cttgttttcc tttttctgct tttaccacat agtattttgt
    10321 acctttaaca ttagcaaatt cttttgactc ttttttagaa cctattttag catttgcccc
    10381 agcttctcca gttatccctg tatctccttt ttttccatct tgtccatttc caccacgtcc
    10441 accatctacg taaagtttaa gttctcctct tttttctatt tctttaaaag ataatatgat
    10501 atcacctgaa gtacctgcat tattgccatt tcttccattc attccgtcgt aaatagaact
    10561 ttctccaacc aatttttctg catccttacc agatacgttg attgaataag tcccttctgg
    10621 tatatgtaat ttttctgtaa gtagtacaat attcttacct gagaagactt ctttctccat
    10681 attacagtca attttcaact cacaatctcc atacatatat atagatgaca aaatattaag
    10741 ttgataacca gatattattt tagtcaaatc cgataaattc actttaggta taggaatata
    10801 tatagaaagt acattttctt tctcatatga ttttatatat gtacaacttt gaatcttctc
    10861 tatcctatac tctgcatctt caagcctttt ggcttcttct tccctatatt cttcttctgc
    10921 tatccaatat ttgatatctt tcaatgtatc ttctaaagta ttttttagca atgattttaa
    10981 caactcactt agaccaacaa tatgagtctc actacagtaa ctatttaact ctaaaagaag
    11041 acgtgtttct tctaatatat tcactttctg tacgccatca ccatatgaaa gttcttgtac
    11101 aaactccata agctctttca tattattagt cctactacat aaatctgtat acatcttatc
    11161 taattgaatc tttttatctt ttagactatt tatagaagat agaatggaaa tagaatcttc
    11221 catacctttt ttaacacttt ctatataagg aactaaaagt ttttctaggt ctttataggt
    11281 tacatcaaca gctttttgta tttctatttt acaagaagca gtaagcatca atcccaaatc
    11341 tgttttttca aatgtttcag gtagattttt catttcattc aacaactttt ctggctgttt
    11401 atctagtgga ttacataatt taatagatac accatctgat aatgctacat ttaataagta
    11461 cactaattcc ttacaaccgt cgtaatcttt ctcttgacta tctaggtctt cttttgcttt
    11521 ttcaaatata tttcttattt tttcttctgt tacattacca attagtgttt taggtatttt
    11581 attgataaga aagaagaaat tttctttata ctttgcataa tcaccaagca tatctttcaa
    11641 atttttgcct gtttctatca ataatgtatg tctagagtcc agtagggatt gatagtctaa
    11701 aataactacc atccctttta cctttgaaga tgtggaaata gtttccctta caagcatagc
    11761 accagcaatc atatactctg ctccacggtt gtcaaaaaaa cctgggcaat cacataatga
    11821 aaaatctcta tttgcaatat ctacaacttc tggacataaa gtcttagaat atacaccatt
    11881 tccaatttcg acagctgaat cttttgcagt tatatatatt tgtccagtct cttcatccgt
    11941 tttttcttcc atcacacagc cttttaaata atttatacaa gtactctttc caacgcctga
    12001 agcacctagg aataacaaaa ttgacttttc ttttaagtca tattttctaa gctcgtctct
    12061 tccttcttcc aaaaggttta cgatttcact ataaagttta tttgccatac atccatccct
    12121 ttctatattt cggaacaata gccttgatat ttttctttaa taagattctc ttctaccttt
    12181 gaaacagctg ttgataactt agtgtttgga gcattaaatc ctccattgtc tcctttatct
    12241 ccatattcag cgtattctct tgctggtcct gttttacctt cattccagct tccaccacta
    12301 agttccacac gataataaac tactttttct atcttataat cccaacttgt aaaatatcct
    12361 gtatagtata cagtcttatc tataccactt ggagaaaatg aaagtgtatt atatccgtga
    12421 tatccaccac ttcctccata tccacctaaa cctggtctac catttggacc tgcttctcca
    12481 tctttcactg ttatatcaca atgattaatt attctatttt tttgttcctc tgaatctgaa
    12541 atgaagtaaa tattaccttt cttaccagca tttccacctc ttccagcatt tccaccagct
    12601 ccaccttttt cacctttttt gccacttgta cctttttcaa aatacccttt aaacatctgt
    12661 ccgtggaaat aacttgtata tcctgcatca gctccattac ttccagaagc tcctgtttca
    12721 ccatttttcc catctattcc tttagaacca tttcctccat ttgcaatcag tgttagacga
    12781 taatttctaa tttcttcttt aatatataag taaatattac ctgcatctct accatcttca
    12841 ccatttttcc catacataga attatctaca agttctccac tctttccact tacattaatt
    12901 tttacattac tacctacatc aagattgtta gaagcaagaa taatattttt ctctgatgca
    12961 aagtccattc caaggttatc atccatataa atagtatctt tagcaaagaa cataaaagta
    13021 gaaacattgt catctacttt tctatttttg aaatattcca atacactgct aattactaag
    13081 tttggaatta atgctcttat ttcaatacct ttcttatcat taatattaat tgtttttact
    13141 ataaatggat ttaaaatgta tttaccaata aattctctaa ttatctcatt accttcttct
    13201 ccatatgcac ttaatcttgt tatactgtct tctaactctt cattttgtgt aactgtaaca
    13261 tttgtcaaaa ttgaattaaa atcacttaca ctatatacat aatccttttt atttgcaact
    13321 tccattaatt tttctctatt tggtaatgta aatacctgtt tttgcacttc ataagaaact
    13381 aaaacttttt ctaaattctt tcttattata atgaagtttt gttccattat cttcttactt
    13441 gccttttcta taataaattc catatgtaca gaaggtaata aaatttcccc agttccatgt
    13501 tctgtatatt tacttaaagc atcaattata tctaagtttc cttttatctt attttgtaat
    13561 ttcatgcaac tatcttgtaa gaataagcca ctttgtttgg ctaatacgtt gataaaactc
    13621 tctatagtaa gacttgttgt actttccaac cagtttttaa atacttttac aacctcatca
    13681 tattttgcta tttcttgaat cattgttata cttccaataa tattgataaa atcttctgta
    13741 tacgccttaa cataatcttc taggctagat acgatattgt tttgaatttc ttttatcaac
    13801 gtatctaaaa ctaatcttgc atctgcacta attggatatt ggaatgctga tgtcttatct
    13861 ttaacttcat ctaattctag tacttgcttt aataagcctt ccctctcatc tgatgcaagt
    13921 ggcttacaaa gatgaatatt ttttccatta tcttttagca attcttcaaa taaccaagct
    13981 ttttcctcta tattactgtc taaattttcg cataattgtt catattttct ttttataaca
    14041 ttatatattt gtttttctgt tcttcctaag aaactttttg aaatgaaaaa agttatacta
    14101 cccttaaaat tttctttatg aagacacata tttaactgtt ggaacaggtc tagtactttt
    14161 gtaagtctag aatctaatat atctgcttct ggtatcaata caattactgc acatatagat
    14221 gcagaatttt taaatacttc acctaatgaa actgctgcac taatatcatg ggctactccc
    14281 ctattgtcgc taaaacctgc agtatcacat aattttgcat ccattccttc tattggtttt
    14341 ctcatattta caatgttcgg gtataaagtt tgagaaacag ctcctgaacc aatctctgcc
    14401 acagggtcta agcaatcaat atatgatata ccagtatctt catcttctac ttccatcatt
    14461 acacatcctg caagatagtt aattgcagta cttttgccac atcctgtccc accaagaatt
    14521 agaattaaat tttgattttc agccttattt attgctactt ttccttcatt taataagagt
    14581 gttatttctt ctaaaagttg attgttattt tctggcattt attttccccc ttggttatat
    14641 attttattta aataaagttt aatcccttaa gtagattgca aagtttttag actatattta
    14701 aatttatttt ttatttttaa taaatttgca ttttcactaa atttgctttt tcactcaata
    14761 attattcttc tttgtttatt aaaataactg tgttgtataa tataatcttt accaacacag
    14821 ttattttatt taatgcgctt attttattgt attttatgta atacagttgt tttttatatt
    14881 atatgtccat tattttccta tggttattac atatagattt ttaattatat ttgtaccatt
    14941 aatttaaaca ttcctatact actgcttaag ttttacacct ataccagctt gaatatttta
    15001 tttttatttg cttaaatagt tcaactatac tggtttaaat aggttatctt tatttgctta
    15061 aatagttcaa ctatactggt ttaaataggt tatctttatt tgcttaaata agttcaacta
    15121 tactgattta aataggtttc ctttagttta aataattcac ctatactggt ttaaataggt
    15181 ttcctttagt ttaaataatt cacctatact ggtttaaata ggtttccttt agtttaaata
    15241 attcacctat acctacttga atagttcact tttatttgct taaattctat accaatactg
    15301 gcttaaacat tatactacca tgattgtatt gtctatcttc aactacaata aaaacaggtt
    15361 ctaaatcttt agtagatgtt atttttaatt ctaaaaaaat attatcagtt ggtaatattt
    15421 ttacatcata attatttaaa ttattattcg tgatactatt aaatacatca ccttgacgtt
    15481 caaaaaaaac attatcaact attacaaaat aaacatcttc ttttctacta ttttcaacct
    15541 ctatagtatt ttgtttagtt atatttagtc tcatcttcca cctcctatta atattactta
    15601 attatattaa tacttgtaat tgttgcatta tgttcatcac aaggaagagg tttaaaaaca
    15661 actatctctg ctaaataatt ctgtggagga tttgtaactg aagtagcatt gcaaataaat
    15721 ttaaaatttg ttcctgctac aacttgagta gaaacaataa gtggttcata accaacccct
    15781 tctaacattc ctacagcttc attaaatact tttaaatctt cttcttcgac agctctgaat
    15841 tttgaccatc caccaacaaa aagactcata taaaaacctc ctttatatat atatttattt
    15901 atatatagta gtaccaattt tctcaatatt caatattttc tgtgtaactg gtcaatgaaa
    15961 atgcataact ggataaaaat acttataaaa taacttaact ttacatataa atagcttact
    16021 ttacatgtaa taagattatt ctacaatatg aaatgcatct tgttttgaat atattctaaa
    16081 tacaataatg gatattttca attattataa ttagaaaata tccattactt aacttgtttt
    16141 atttaaaatc acatagagac tttattattt tttttattgc atgaatagct atttcaggta
    16201 tcataaggtg atataccccc tccttgaaac cacaaaaaac atgacaaact ttacgaatct
    16261 tgtcttaaaa aactacaggt acattcctta tttgtggatt ggagtagcca taacaagcaa
    16321 attttacaat ttttatgatg acaaaaatca ataaaatcgc ttagtatata acacgaaata
    16381 ctacttgtta ctattaagaa tagttagata ggaagtatgc attatacaaa taaaggaatc
    16441 aataaagaaa gttaaaacat ccctacaaat taaaaatctg ttacattttt ctatatttaa
    16501 acgttattta cttacaattg gagaaataac aggttttcct tctcggtcaa gtaaaggcgt
    16561 aaatccggct gcatggccat aatagtggaa tacatagttc acacctgttt ctttatccac
    16621 ccaaatctcc gttgcattta tttttccttg tgaatatact ttttcaaatc tatcttttcc
    16681 cattttataa tcctccatat ttttgattgg tctatgcctt cttcacttaa gtagaaaaaa
    16741 tacataactt atttttttaa ttctaatatg agcacgtaaa aatgtcaaac agcttattta
    16801 atttcataac ttcatgttac tgttagtaac cttgtctgtt tacttacact gcattacagc
    16861 accattttat cctttacatt attgtattgc acacagtaaa aatgcttact ttatgggatt
    16921 tttcaactaa aaaaaggatt ttatctccac aaaacattta ctatttgtta caatatacct
    16981 aattttaatc tcatatcaac atttaatgaa gatataacct ttttcaaata ttgtaaagac
    17041 tccttaaaaa tattagaaaa agattctgca aatctctcaa catctccttt ttttaaatac
    17101 ctaagtatct caaaacatac atcaaaacgt gttttcaaaa tttcattata ttcttcatga
    17161 aaaaatctct ctaaatagat tccaaatatc atatattgtt ttaaatgcaa aaatgtttcc
    17221 tgtaatacat aaatttgtgt ataatctact atcttacaaa gtaaaacaaa atctggataa
    17281 accttacact cttcacaatt attttgtaag taccttaaat gagtttctat tttatctaca
    17341 gctatcaaat ccattttatt tcctagtaaa agtactacat cctcaagagt aatcgtaaca
    17401 aactccatag catccaaaag caaactcaac cttttagctc cttcttctgt atttatataa
    17461 tcatcatcat attcagaaaa aagtgttaca tatgttccct tgccattaat tgttttagca
    17521 attcccaatt catttagctc attataggca cttctaactg ttggaagaga tacattatat
    17581 ctattaatta tcgaagtaat tgatggtaga caatctccta ttttaatatt gccttcaaaa
    17641 atttgacaaa ttacatctga aacaattaag tcatataaat aacatttttc caacgttaca
    17701 gaattaaaac tacttgtatc tatactttgt ggagcacgat tttctaccat tttaagtatg
    17761 gattctctac aagctttata catatatgtt aatagttttt taaattcaat tagattcccg
    17821 tccaatccat ataagtaagt ttttcttacc tgctctatgt atttattacg aaaaaagtct
    17881 atacaacaat tatccaagtt atccaagtta tcgaaattat aggccaattg tgaaataatg
    17941 acatcatgct gaataatctc acaaaaatgg tgaatcagga aattatttgt tttacttatt
    18001 agtttattta tatattttag cctttgttct ttaaagacta aataattaga actatttttt
    18061 tccatacaat cgataatctc gtttaattct tcaaagtctt tagatgtaaa tcttttaata
    18121 ttgtacatag caatagaagg aaatattaaa ctaaaaatct tatatatttc tgatatttgt
    18181 aaaatcttct catataataa gtcagattct attgattgtt tactcgtact ctggattaaa
    18241 actggtctag aaccagcttt tgtctctata aagccctttt ctgagagcat ttttattaca
    18301 gtttttatag tattttttga taccatatat tcttctgaca tgctttttat agaatcaagc
    18361 ctatctccaa tagtaatttt accagtataa atttgttgaa ttatatgctc aaaaataatt
    18421 aaatattgtg gttttctctt catatttttc ctcttcgata aattagattt attgtttttt
    18481 gtaaggtata tttactttta tttaataggg caaaatagtt gcttattttt tcctcataat
    18541 ctataattaa tataaaccat ttatatatat ggtctaagtt ttataatact gtaatgcagt
    18601 aaaatagcat attgtagcta tactacagca aattaacata ccattgtagt atattaattg
    18661 gaataatttt aagtacttca aaattccatc aaaacatata tgtgtttttg atattatgta
    18721 tattcgttaa tagaatagtt atacttgttt tacataataa attatatgat acttgctata
    18781 tgtaat
//
LOCUS       DBGJFF010000073        18576 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.074, whole genome shotgun sequence.
ACCESSION   DBGJFF010000073 DBGJFF010000000
VERSION     DBGJFF010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18576
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.074"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            98..1699
                     /locus_tag="ACOIAF_002472"
     CDS             98..1699
                     /locus_tag="ACOIAF_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp11"
                     /protein_id="HGZ2669479.1"
                     /translation="MKMNKKILSLGLAVSLILVNFKSVNASSVVEKIYGKDRYETAAK
                     IADKQTYETVILVNTEKSLADGLSASGLSGATKAPILFTQQNKIPADTNRCLKNIKKA
                     YIIGTEDTISKSVEKELDSKNIEVKRIGGEDRLKTSYLIAKEIATIKKVDKVLLTNAY
                     SGEADAMSVSSVATRDGAPIILTDGKSVPFDVKNIQSYCIGSEEIMSNPLVKNTNSVR
                     IEGTDRFETNKNVIDYFFNSADGFYVSDGYQLVDAIAAAPLTKNSPMVLVNDGSDKTV
                     LEGAKNITSVGEINEKVIQQCINASKSNGQPPTITVGSTEIYKGEKFDTGKLNIVAKD
                     NTGKVLPIEVDGFIDTNRVGTYILTLKATDEWGKSTGKRVEIKVLDDKSHDYNSPEFK
                     KMVSTEMYNLINSYRKEKGKEPLVVSSRLEGMANAWSKYMMDKKVFAHYIDGKNAPQV
                     FSEFGMRSEENIAYIYIDSKNVQTTQDAKDLAKAIFEVWKKSPEYNANMLSDEFYSTG
                     FGLYILSDGQVHATQEFLNGNEGSL"
     gene            1918..3507
                     /locus_tag="ACOIAF_002473"
     CDS             1918..3507
                     /locus_tag="ACOIAF_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp12"
                     /protein_id="HGZ2669480.1"
                     /translation="MKVNKRVLSIGLTISLIMAGAPNINALSSIEKIQGKDRYETAAK
                     IAQKQTYENVVLVNTDNTLADGLSASGLAGTVKAPILLSQRNSIPSDTEKMLKDVKKV
                     YIIGTEDSIGKSVENELKQKGIEVKRIGGNDRIETSYLIAKEIASIKPIDKVFITNGY
                     TGEADAMSASSVASRDGAPIILTNGKNVPFEKKEGVQCYALGSEEIISNDLVKKTNAV
                     RLAGEDRFETNKKVIKHFYSSAKEFYLSKGYQLVDAVAGSSIAKNAPIVLVDGNSDKS
                     VLRSADKITALGGIDEKTLEQCLSASSLDASAPTITVGNLNIYQGDKFDISKLNIVAK
                     DSNGNDLTPELIGNIDTDKVGKYKVTIKATDIGGKTTSISVEVNVLEYKTNDMNSSEF
                     KRMVSSEMYNLVNSYRKEKGKEPLQVSKNLEGLSNSWSKYMADKGQFSHVIDGKKSVE
                     VFTGYGLRSEENIAFVPLVTKSTYTTKDAREVANVIFTVWKKSDKYNENMLNSDFVYT
                     GFGLYILPNGEVYATQEFLNK"
     gene            3719..5860
                     /gene="slpA"
                     /locus_tag="ACOIAF_002474"
     CDS             3719..5860
                     /gene="slpA"
                     /locus_tag="ACOIAF_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer protein SlpA"
                     /protein_id="HGZ2669481.1"
                     /translation="MNKKNIAIAMSGLTVLASAAPVFAVDAPVKAPEGTDYVDNYTIV
                     QSKYEKALKKLQDGLVNGEIKSIDVYFDGSKIAQAITDKDELFRLVDAKLDGLGDGDS
                     VQFTVHYAGDAGKYTLETLDALKTAAVSASAEAIPPGGEGVVGVITNSDPSGTGTNAK
                     GTALKYSDIVKTAKFTGNGVVLTLQDSLSSVKLGEVKASANTYETVTKVAFDDKNTLT
                     KTVSVGDKLLDLSKPLVIKEIKNGTLIIDLPENTTSSSSDSSINLKVVNAKEQTIDLD
                     ASSYKSAEDIAKTYAFDTNEVKNLYTAIEELQKDNVKTNKVQLIDGKYQVTLWADGKR
                     LTTKSAAQPSKLAGDNSPLCLVIKADKLKDLKDYVEDLRNANNGYSNTVTVAGDDRIE
                     TAIELSRKYYNNTDEDNAVKKAPVDSVVLVGSQAIVDGLVASPLASEKTAPLLLTSKD
                     KLDSNVKSEIKRVMNLKSSTGIDSSKKVYLAGGVNSISKEVENELKDMGLKVTRLSGD
                     DRYATSLEIADEIGLDNDKAFVVGGTGLADAMSIAPVASQLDGKNATPIVVVDGKAKE
                     LSSAAEDFLDDAQVDIIGGKNSVSKDMEDAIDDATGKSPNRISGDDRQATNAEVLKES
                     DYFPDGAVNYFVAKDGSTKEDQLVDALAAAPVAAHFEAPIILATDSLSSDQNVAISKA
                     LPSGKSGDNLIQVGQGIASSVITKIKDLLDM"
     gene            6108..8453
                     /gene="secA"
                     /locus_tag="ACOIAF_002475"
     CDS             6108..8453
                     /gene="secA"
                     /locus_tag="ACOIAF_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HGZ2669482.1"
                     /translation="MSVLDTILDKADEQEIKKLNLIVDKIEALEEKIQNLSDDELKEM
                     TNVFRNRLNNGETLDDILIEAFAVVREVSKRKLGMRQYRVQLIGGIVIHQGKIAEMKT
                     GEGKTLVEVAPVYLNALTGKGVHVITVNDYLAERDKELMRPVYESLDMTVGVIISNQD
                     SSTRKEQYKCDITYGTNSEFGFDYLRDNMVPDLSHKVQRELNFAIVDEVDSILIDEAR
                     TPLIIAGDGDEDLKLYELANSFVKTVKEEDFEMDRKDKTIALTANGISKAESFFGITN
                     LTDIKNIELYHHINQALRGHKLMEKDVDYVISNGEVMIVDEFTGRVMDGRRYTDGLHQ
                     AIEAKEGVEIKNESKTMATVTYQNFFRLYDKLSGMTGTAKTEEGEFESIYKLNVVQIP
                     TNKPVIRADLHDKVFKTEEEKYSAVVEEIIRIHKTRQPILVGTVSVEKSEKLSKMLKK
                     QGIKHQVLNAKQHDKEAEIISKAGKLNAITIATNMAGRGTDISLGAGDKEEEQEVKDL
                     GGLYVIGTERHESRRIDNQLRGRSGRQGDPGTSRFFVSLEDDVIKLYGGKTIEKLMKR
                     TSSKENTAIESKALTRAIERAQKGVEGKNFEIRKNVLKYDDTINEQRKVIYNERNKVL
                     NDEDIQEDIQKMVKDMIQEAGETYLIGRKRDYYGYFKHLYSTFMPADTLLIPGVDKKS
                     VQEIIDSTYEISKRVYDLKKMMIGIDKIAELEKTVLLKVVDQYWIDHIDAMEQLRQYI
                     GLKSYAQKDPFKEYALEGYDMFEALNKNIREATVQYLYKFN"
     gene            8868..10739
                     /locus_tag="ACOIAF_002476"
     CDS             8868..10739
                     /locus_tag="ACOIAF_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp2"
                     /protein_id="HGZ2669483.1"
                     /translation="MNKKNLSVIMAAAMISTSVAPVFAAETTQVKKETITKKEATELV
                     SKVRDLMSQKYTGGSQVGQPIYEIKVGETLSKLKIITNIDELEKLVNALGENKEFIVT
                     ITDKGHITNSANEVVAEATEKYENSADLSAEANSITEKSKTETNGIYKVADVKASYDS
                     AKDKLVITLRDKTETVTSKTIYVGIGDEKVDLTANPVDSTGTNLDPSAEGFRVNKIDK
                     LGVAGAKNIDDVQLAEITIKNSDLNTVSPQDLYDGYRLTVKGNMVANGTSKSISDISA
                     KDSETGKYKFTIKYTDASGKATELTVESTNEKDLKDAKAALEGNSKVKLIAGDDRYAT
                     AVAIAKQTKYTDNIVIVNSNKLVDGLSATPLAQSKKAPILLASDNEIPKVTLDYIKDI
                     IKKSPSAKIYIVGGESAVSNTAKKQLESVTKNIERLAGDDRHTTSVAVAKAMGSFKDA
                     FVVGAKGEADAMSIAAKAAELKAPIIVNGWNDLSADAIKLMDGKEIGIVGGSNNVSSQ
                     IENQLADIDKDRKVQRVEGETRHDTNAKVIETYYGKLDKLYIAKDGYGNNGMLVDALA
                     AGPLAAGKGPILLAKTDITDSQKNALSKKLNLGAEVTQIGNGVELTVIQKIAKILGW"
     gene            10846..11553
                     /locus_tag="ACOIAF_002477"
     CDS             10846..11553
                     /locus_tag="ACOIAF_002477"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HGZ2669484.1"
                     /translation="MVDATLFFTPHQDDETLSMGSAIIEHVEKSDTHVILCTDGSKSI
                     IRKVLDDGGSCSYHIKDIHKYSLSENEFSKDRDEEFKDSCEAMGVKESNIHIEDNRAH
                     DGELSKEKAREIILKYLEEYPDAKVKTVTPFKASGIHEDHRALGEAALELYKEGKIKD
                     LRFYVEPYDYKDFKKVNPNVEVWKVLPSQEEKLLSAMNAYKKWNPESGHYAIGYHSVK
                     SHFDELAINKIQYVHAP"
     gene            11586..13424
                     /locus_tag="ACOIAF_002478"
     CDS             11586..13424
                     /locus_tag="ACOIAF_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HGZ2669485.1"
                     /translation="MKISKKIVALLTITFLTITLYGNISNASTKDTLTGSGRWETAIK
                     ISQAGWKKSENAVLVNDNSIADALSATPFAKAKDAPILLTQSNKLDSRTKAELKRLGV
                     KNVYLIGGSIALSSEIEKQLNAENINFERISGNSRYDTSLKLAEKLDREKSISKIVVV
                     NGEKGLADAVSVGAIAAQENMPIILSDSDNGTEVADNFIDSKDIEKSYVIGGTYSISN
                     SVERSLPNATRIAGSGRSETNAKIIEEFYKDTDIKNIYVTKDGTRNKNDLIDSLAVGV
                     LAAKNSSPIVLSGNKLDATQKDVLNTKIIDKVTQIGGLGNENAVEDILDMQEETKYTV
                     ETIDELNVAIKKADANDTIKFIPEKDKNINSSFKIETDKAIKVEFDGVYRQSITINMP
                     NGDVDNLGEINGTIEIRNIKNNSLVNRGSIQDLDISTENGCKIENESSGEIWLITILS
                     NAKGVYIINNGDITKISNSSSDVIIKNSGDIDIITGSEEPAISGRKPKVNDTDIDDNK
                     LARGLSVKTESCYVPEEDYIMVTVLSEPRNLDYNIYYRVVDNKPSAMSVGEKISPKNW
                     DAVPKFNKAFLEKAKDGSYIEVVEINSSTNKVSRWGRSNATDDDFR"
     gene            complement(13462..13857)
                     /locus_tag="ACOIAF_002479"
     CDS             complement(13462..13857)
                     /locus_tag="ACOIAF_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HGZ2669486.1"
                     /translation="MILKKHRETILYLFFGAFTTLVNIVSYLFFTRVILFNFMVANAL
                     AWILAVLFAYVTNKFFVFESKRIEIKFLFKEFLSFVSFRLLSGVVEMLIMYVMIDLLF
                     VNDVIVKVFTNIVVIVLNYLFSKMIIFKK"
     gene            14084..16495
                     /locus_tag="ACOIAF_002480"
     CDS             14084..16495
                     /locus_tag="ACOIAF_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp84"
                     /protein_id="HGZ2669487.1"
                     /translation="MRKYKSKKLSKLLALLTVCFLIVSTIPVSAENHKTLDGVETAEY
                     SESYLQYLEDVKNGDTAKYNGVIPFPHEMEGTTLRNKGRSSLPSAYKSSVAYNPMDLG
                     LTTPAKNQGSLNTCWSFSGMSTLEAYLKLKGYGTYDLSEEHLRWWATGGKYGWNLDDM
                     SGSSNVTAIGYLTAWAGPKLEKDIPYNLKSEAQGATKPSNMDTAPTQFNVTDVVRLNK
                     DKETVKNAIMQYGSVTSGYAHYSTYFNKDETAYNCTNKRAPLNHAVAIVGWDDNYSKD
                     NFASDVKPESNGAWLVKSSWGEFNSMKGFFWISYEDKTLLTDTDNYAMKSVSKPDSDK
                     KMYQLEYAGLSKIMSNKVTAANVFDFSRDSEKLDSVMFETDSVGAKYEVYYAPVVNGV
                     PQNNSMTKLASGTVSYSGYINVPTNSYSLPKGKGAIVVVIDNTANPNREKSTLAYETN
                     IDGYYLYEAKANLGESYILQNNKFEDINTYSEFSPCNFVIKAIIKTSSGQATSGESLT
                     GADRYETAVKVSQKGWTSSQNAVLVNGDAIVDALTATPFTAAIDSPILLTGKDNLDSK
                     TKAELQRLGTKKVYLIGGEDSLSKNVQTQLSNMGISVERIAGSDRYKTSISLAQKLNS
                     IKSVSQVAVANGVNGLADAISVGAAAADNNMPIILTNEKSELQGADEFLNSSKITKSY
                     IIGGTATLSSNLESKLSNPTRLAGSNRNETNAKIIDKFYPSSDLKYAFVVKDGSKSQG
                     DLIDGLAVGALGAKTDSPVVLVGNKLDESQKNVLKSKKIETPIRVGGNGNESAFNELN
                     TLLGK"
     gene            16792..18369
                     /locus_tag="ACOIAF_002481"
     CDS             16792..18369
                     /locus_tag="ACOIAF_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp5"
                     /protein_id="HGZ2669488.1"
                     /translation="MKAPKTILTILTIALTLSSISIIPSYALTEEKLIGNGRYETAVK
                     ISQKAYSSSSNVVLVNDNSLADALSATPFAKAKGAPILLTESDKLDDRTEKEIKRLGA
                     KDIYLIGGTAVLNKDIENKLKGNGLNVERINGKDRYETSLILANKLKDIKDIKEVAVV
                     NGEKGLSDAVSVGAPAAQNKMPIILSSPKDGVEAFDKFIRDEKVIKAYVIGGTTSVSR
                     AVEKSLPNAERVSGKDRNETNAKVIEKFYTDTNLSNLYVTKDGSKNENQLIDSLAVGV
                     LAAKNESPVVLVGNKLNTKQRDILSTKKLNTITQVGGNGNEEAFDEIKSLQEKTVFEA
                     KTVEELTDIINIASPNDIINFKPKENTVNEAFRMVTNKPITVNIKGDCSKTLTVDMPN
                     GEVNNYATLVNVIVRNIGEGGFNNHDTITILSVRDKNGRVIENTRNSDIDTLMILASA
                     NDTKLINDGYIGKLIDNSSNSDITNNGTIDKKVNQVEDLEAKVDSIEKAIDSISQKVN
                     KIQDILDKLGFLKKFLS"
BASE COUNT         7669 a         2095 c         3410 g         5402 t
ORIGIN      
        1 catatcatat atactctata atatatggaa aataaagaga aaataaatgg aaaataatca
       61 caatataaat ataaaatata aaaatcatca gggggatatg aaaatgaaca aaaaaatatt
      121 atcattaggt ctagcagtat cattaatctt agtaaacttc aaaagtgtaa atgcatcatc
      181 agtagtagaa aaaatatacg gtaaagatag atatgaaaca gcagcaaaga tagctgataa
      241 acagacttat gaaacagtaa ttttagtaaa tacagaaaaa tcacttgcag atggattaag
      301 tgcaagtgga ttatcaggag ctacaaaagc tccaatacta tttacacaac aaaataagat
      361 accagcagac acaaatagat gtctaaaaaa tatcaaaaaa gcatatataa taggtacaga
      421 agatactata agtaaatcag tagaaaaaga actagattct aaaaatatag aagtcaaaag
      481 aattggtgga gaagatagac taaaaacaag ctatttaata gctaaagaaa tagcaactat
      541 aaaaaaagtg gataaagtac tattaactaa tgcatatagt ggagaagcag atgcaatgag
      601 tgtatcatca gtagctacta gagatggagc tccaatcata cttacagatg gaaagagtgt
      661 gccttttgat gtaaaaaata ttcaatcata ttgtataggt tcagaggaga taatgagtaa
      721 tcctctagtt aaaaatacaa actcagtaag aatagaagga actgaccgat ttgaaactaa
      781 caaaaacgta atagactact tttttaatag tgcagatgga ttctatgtat cagatggata
      841 ccaattagta gatgcaatag cagccgcacc acttactaaa aactctccaa tggtactagt
      901 aaatgatgga agcgataaaa ctgtattaga aggagctaag aatataacct ctgtaggtga
      961 aataaatgag aaggtaatac aacagtgtat aaatgcttca aagtcaaatg gacaaccacc
     1021 aacaattaca gttggaagta cagagatata taagggtgaa aagtttgaca ctggcaagtt
     1081 aaatatagtg gctaaagata acactgggaa ggtattacca atagaagttg atgggtttat
     1141 agatacaaat agagtaggta catacatatt gacattaaaa gctacagacg aatggggaaa
     1201 aagtacagga aaaagagtag aaataaaagt gttggatgat aaatctcatg attacaacag
     1261 cccagaattt aaaaaaatgg tatctactga aatgtataac ttaatcaatt cctatagaaa
     1321 agaaaaaggc aaagagcctt tggtagtgtc tagtaggcta gaaggtatgg caaatgcatg
     1381 gtctaagtat atgatggata aaaaagtatt tgcacattat atagatggaa aaaatgctcc
     1441 acaagtattt tctgagtttg gaatgagaag tgaggaaaat atagcatata tttatataga
     1501 ttctaagaac gttcaaacga cacaagatgc aaaagacttg gcaaaggcta tatttgaagt
     1561 gtggaagaag tctccagagt acaatgcaaa tatgcttagt gatgagttct atagtactgg
     1621 atttgggctt tatatattat ctgatggtca agtgcatgct actcaagagt ttttaaatgg
     1681 caatgaaggt agcttgtaaa aaattccaca aatcttgttt ttttgttaat ttactgatat
     1741 tataaaatga aaatcacatc taaagtactt tttgtaatct gttttgttat tgctttacag
     1801 gttatggtat tttaggtgtt ttttgtttta gtagtttata aattttgagg gaatcactac
     1861 tgaggatgta atttatgatt agctgaatta tataaaaaaa ttagtaggag gagttcgatg
     1921 aaggtaaata agagagtatt atcaatagga ttgacaatat cattaataat ggcaggagca
     1981 ccaaatatca atgcactttc gagcatagaa aagatacaag ggaaagatag atatgagaca
     2041 gctgccaaaa ttgcacagaa acaaacatat gaaaatgtag tattagtaaa tacagataat
     2101 acactagctg atggattaag tgcaagtgga ttagcaggaa cagttaaagc acctatatta
     2161 ttatcacaaa gaaacagtat accatcagac acagaaaaaa tgttaaaaga tgtaaagaag
     2221 gtttatataa taggaactga agattcaata ggaaaatcag ttgaaaatga attaaagcaa
     2281 aaaggaatag aagttaaaag aataggtggt aatgatagaa ttgaaacaag ttatcttata
     2341 gcaaaagaaa tagcttcaat aaaacctata gataaggttt ttataactaa tggttataca
     2401 ggtgaagcag atgctatgag tgcgtcatca gtagcatcaa gagatggagc acctataatt
     2461 ttaactaatg gtaaaaatgt tccatttgag aaaaaagaag gtgtgcaatg ttatgctctt
     2521 ggttcagagg agataataag taatgattta gttaaaaaaa ctaatgcagt tagattagct
     2581 ggagaagata gatttgagac taataaaaaa gttataaaac atttttatag ttcagcaaaa
     2641 gaattttatc tttctaaagg ttatcaatta gtagatgcag ttgctggttc ttctatagca
     2701 aaaaatgcac ctattgtatt ggttgatgga aatagtgata agtctgttct tagaagtgct
     2761 gataaaatca cagcactagg aggaatagat gaaaagacgc tagaacaatg tttaagtgca
     2821 tcatcactag atgcaagtgc accaacaata actgttggaa atttaaatat ttatcaaggt
     2881 gataagtttg atataagtaa acttaatata gtagcaaagg atagcaatgg taacgatttg
     2941 acaccagagc ttataggaaa tattgatact gataaggtgg gtaaatacaa agttactata
     3001 aaagctactg atattggtgg aaagactacc tcaataagtg tagaggtaaa tgtacttgaa
     3061 tataagacta atgatatgaa tagtagtgag tttaaaagaa tggtttctag tgaaatgtat
     3121 aatttggtaa attcatatag aaaagaaaaa ggtaaagaac cattacaagt ttctaaaaac
     3181 ttagaagggc tttctaattc ctggtctaag tacatggcag ataaaggcca gttttcacat
     3241 gtaatagatg gtaaaaaatc tgttgaagta tttactggat atgggttgag aagtgaagaa
     3301 aatatagctt ttgtaccact tgttacaaag agcacttata caacaaaaga tgctagagaa
     3361 gtagcaaatg taatatttac agtttggaaa aagtcggata aatataatga aaatatgcta
     3421 aatagtgatt ttgtatatac tgggtttgga ctgtatatat tgcctaatgg agaagtgtat
     3481 gctactcaag aatttttaaa taagtaaaat taaatttttt aagttattaa atattgaaat
     3541 ttaagggtat aaaaactcat atgcttggga aaaataataa tttcaaaaaa atgccttgca
     3601 aaaagaataa aaatggatta ttatagagat gtgagaaata ttaggaatat atggatgatt
     3661 attctatgta cataataaga gatgtaattt taatataatg ttgggaggaa tttaagaaat
     3721 gaataagaag aatatagcaa tagctatgtc aggattaaca gtgttagctt cggctgcacc
     3781 tgtatttgct gtggatgcac cagtaaaagc cccagaaggt acagattatg tagataacta
     3841 cactatagta caaagcaaat atgagaaagc tttaaaaaaa ttacaagatg gattagtaaa
     3901 tggtgaaata aaatcaattg atgtttattt tgatggttca aaaatagctc aagctattac
     3961 tgataaggat gagttattta gacttgttga cgcgaaatta gacggtttag gtgatggaga
     4021 ttctgttcaa tttacagtac actatgcagg tgatgctggt aaatatacat tagaaactct
     4081 tgatgcttta aaaactgctg cagtttctgc ttctgctgaa gcaattccac caggtggaga
     4141 aggtgttgtt ggtgtaatta ctaatagtga tcctagtgga acaggaacaa acgctaaagg
     4201 aacagcacta aaatattctg atattgttaa aacagcaaaa tttactggta atggtgttgt
     4261 tttaacatta caagatagtt tatcatctgt taagcttggt gaagtgaaag catcagctaa
     4321 tacttatgaa acagtaacaa aggttgcttt tgatgataaa aatactctta ctaaaacagt
     4381 aagcgtagga gataaattat tagatttatc aaaaccactt gttataaaag aaataaaaaa
     4441 tggaacttta attatagatc ttccagaaaa tactacaagt tcaagttcag attcatcaat
     4501 aaatcttaaa gtagtaaatg caaaagagca aacaatagat ttagatgcta gttcatataa
     4561 atcagctgaa gatatagcaa aaacatatgc ttttgataca aatgaagtta aaaatcttta
     4621 tacagctata gaagagttac aaaaggataa tgtaaaaact aataaagttc aattaataga
     4681 tggtaagtat caagtaactc tttgggcaga tggaaaaaga ttaactacta aatcagctgc
     4741 acaaccttct aaattagcag gtgacaattc tccactttgt ttagtaataa aagctgataa
     4801 attgaaagat ttaaaagatt atgttgaaga tttaagaaat gctaataatg gttattcaaa
     4861 tactgttact gtagcaggtg atgatagaat agaaactgca atagaattaa gtagaaaata
     4921 ttacaataat actgatgaag ataatgctgt taaaaaagca ccagttgatt ctgtagtatt
     4981 agttggttct caagcaatag ttgatggtct tgttgcatct cctttagctt cagaaaagac
     5041 agctccttta ttattaactt caaaagataa attagattca aatgtaaaat ctgaaattaa
     5101 aagagttatg aatttaaaat cttcaacagg aatagatagt tctaagaaag tttatttagc
     5161 tggaggcgtt aattctatat ctaaagaagt agaaaatgaa ttaaaagata tgggacttaa
     5221 agtaacaaga ttatctggag atgatagata tgctacttca ttagaaatag ctgatgaaat
     5281 aggtttagat aatgataaag catttgttgt aggaggaaca ggtcttgcag atgctatgag
     5341 tatagctcca gttgcttctc aattagatgg taaaaatgct acgccaatag ttgttgttga
     5401 tggaaaagct aaagagttaa gttctgcagc agaagatttc ttagatgatg ctcaagttga
     5461 tataataggt ggaaaaaata gcgtttctaa agatatggaa gatgctatag atgatgctac
     5521 aggaaaatct ccaaatagaa ttagtggaga tgatagacaa gcaactaatg cagaagtatt
     5581 aaaagaatca gattatttcc cagacggagc agtaaattat tttgttgcta aagatggttc
     5641 tactaaagaa gatcaattag ttgatgcatt agcagcagcg ccagtagcag ctcactttga
     5701 agctccaatt atattagcaa ctgattcatt atcttcagat caaaatgttg caataagtaa
     5761 agcattacct tctggaaaat caggagataa cttaatacaa gttggtcaag gtatagcaag
     5821 ttcagtaata actaagataa aagatttatt agatatgtaa tataagtttt aaatataaag
     5881 taaaaaagat tcctcaaaga ggaatctttt tttgttttta aaccataata aaaataaaag
     5941 agagactaaa tacttagtct ctcttttttg tgagaaatac ttaaatgatt aaaagatgta
     6001 attcattcaa gtatgattat tatactataa ataaaaaaag ttgtctacta ttctacattt
     6061 ttaaggtata ataatagttg tatgataaaa aaggaggtaa gaaatacatg tcagttttag
     6121 atacaatact tgataaggca gatgaacaag aaattaagaa attgaattta atagtagata
     6181 aaatagaagc tttggaagaa aaaatacaaa atttatctga tgatgaactg aaagaaatga
     6241 ctaatgtatt taggaataga ttaaataatg gagagacttt agatgatata ctaattgaag
     6301 cttttgcagt tgtaagagaa gtatcaaaaa gaaaattagg aatgcgtcag tatagagtgc
     6361 aattaattgg tggtatagta atacatcaag gaaagatcgc agagatgaaa actggtgaag
     6421 gtaaaactct agttgaagta gcaccagtat atttgaatgc ccttacaggt aagggtgtac
     6481 atgtaatcac agtaaatgat tacctggcag aacgtgataa ggaacttatg cgtccagttt
     6541 atgaatctct tgatatgaca gtaggagtaa ttatatctaa ccaagactca agtacaagaa
     6601 aagaacaata taaatgtgat ataacttatg gaacaaatag tgaatttgga tttgactatt
     6661 taagagataa tatggtgcca gatttatctc ataaagtaca aagggaacta aattttgcta
     6721 tagtggatga agtcgactca atattaatag atgaagctag aaccccactt attattgcag
     6781 gagatggtga tgaagattta aaactttatg agttagcaaa tagctttgta aaaactgtta
     6841 aagaagaaga ctttgagatg gatagaaaag ataaaactat agcattaaca gcaaatggta
     6901 taagtaaagc tgagtcattt tttggtataa caaaccttac tgatataaag aatatagaat
     6961 tatatcatca tataaatcaa gctttaagag gtcataagct tatggaaaaa gatgttgact
     7021 atgttatttc aaatggagaa gtaatgatag ttgacgaatt tacaggaaga gtaatggatg
     7081 gtagaagata tacagatgga cttcaccaag ctatagaagc caaagaaggt gttgagataa
     7141 agaatgaatc taagactatg gctactgtta cttatcaaaa tttctttaga ttatatgaca
     7201 aactttctgg tatgactggt actgcaaaaa cagaagaagg tgaatttgag tcaatctata
     7261 aattaaatgt tgtccaaata ccaactaata aaccagtgat tagagctgat ttacatgata
     7321 aggtatttaa aacagaagaa gaaaagtata gcgctgttgt agaagaaata ataaggatac
     7381 ataagactag acagccaata cttgtaggaa cagtttccgt tgaaaaatct gagaagttat
     7441 ctaaaatgct taaaaaacaa ggtattaaac atcaagtctt aaacgctaaa caacatgata
     7501 aagaggcaga gataatttct aaagctggta aattaaatgc tataacgatt gctacaaata
     7561 tggcaggtag aggaaccgat atttctctag gtgcaggaga taaagaagaa gaacaagaag
     7621 ttaaagattt aggtggactt tatgttatag gaacagaaag acacgaatca agaagaattg
     7681 ataatcagct tagagggcgt tctggtcgtc aaggagaccc aggtacatca agattttttg
     7741 taagtcttga agatgatgta ataaagcttt atggtggaaa aactatagag aaacttatga
     7801 agagaacaag ttcaaaggaa aatactgcta ttgaaagtaa agcacttaca agggctatag
     7861 aaagagcaca aaaaggtgta gaaggtaaaa attttgaaat aagaaaaaat gtccttaaat
     7921 atgatgatac tattaatgaa caaagaaaag ttatatataa tgaaagaaat aaggtgttaa
     7981 atgatgaaga tatacaagaa gacatacaaa aaatggttaa ggacatgata caagaagcag
     8041 gagaaactta tttaattgga agaaaaagag attattatgg atattttaaa catttatata
     8101 gtacatttat gccagcagat acattactaa tacctggtgt agataaaaaa agtgtccaag
     8161 agataattga tagcacatat gaaatttcaa aaagggttta tgacttgaaa aagatgatga
     8221 ttggtattga taagattgca gagttagaaa aaacagtact tttaaaagtg gttgatcaat
     8281 actggataga tcatatagat gctatggaac agttaagaca gtatataggt cttaaatctt
     8341 atgctcaaaa agacccattt aaggaatatg ctttagaagg atatgacatg tttgaagctt
     8401 taaataaaaa tataagggaa gcaacagtac aatacttata taaatttaac taaaaaagtt
     8461 catgtaattt ttattaaatg aaaaataaaa tattttgatt ctttatattg taattacaat
     8521 taaataatta aaaaatacaa atgaaaaaga agctgaaaat cagcttcttt ttctgtgtga
     8581 gaaatacgga aaaaagagat gcttgaaatt tctttcttcc atttaatatt atactataaa
     8641 gaagatgaaa agtctattag ttataaactg gattattaaa aaaaattaca attttgttac
     8701 ttttttgtat acataaatat taaaaaaata aaatattaga aaaagtttat atcttttggt
     8761 taattattac aataagtctc atttattgaa ataatatcaa atatatatta taattggtaa
     8821 ataaggaaaa aataataaaa aattgaattt tttaggggga aaataccatg aataaaaaaa
     8881 atctttctgt aattatggct gctgcaatga taagtacatc agtagctcca gtttttgctg
     8941 cagaaactac acaggtaaaa aaagaaacaa taactaagaa agaagctaca gaactagttt
     9001 cgaaagttag agatttaatg tctcaaaagt atacaggagg ttctcaagtt ggacaaccaa
     9061 tatatgaaat aaaagttggt gagactttat caaaattaaa aataataact aatatagatg
     9121 aattagagaa attagtaaat gctttgggag aaaataaaga atttattgta actataacag
     9181 ataaagggca tataacaaat agtgcaaatg aagtagttgc agaagcaact gaaaaatatg
     9241 aaaattcagc agacctttcc gctgaagcta actctataac agaaaaatct aaaactgaaa
     9301 ctaatggaat ttataaagtt gcagatgtaa aagcttcata tgatagtgct aaagataagt
     9361 tagttataac tttaagagat aaaacagaaa cagtaacttc taaaactata tatgtaggta
     9421 ttggtgatga aaaagttgat ttaacagcaa atccagttga ttcaactgga acaaatttag
     9481 acccttctgc agaaggattt agagtaaata aaatcgataa actaggtgta gcaggagcta
     9541 aaaatattga tgatgtccaa ttggccgaaa taactataaa aaatagtgac ctaaatacag
     9601 tttcaccaca agatttatat gatggatata gattaactgt taaaggtaat atggtagcaa
     9661 atggtacatc aaagtcaatt agtgatattt cagcaaaaga ttcagaaaca ggaaaatata
     9721 aatttactat taagtatact gatgcatctg gaaaagcaac agagcttact gtagagagta
     9781 ctaatgaaaa agatttaaaa gatgccaaag ctgcattaga aggtaattca aaggttaaat
     9841 tgatagctgg agatgataga tatgcaactg cagtggctat agcaaaacaa acaaaatata
     9901 ctgacaatat agttatagtt aattcaaata aactagttga tggattatca gctacaccac
     9961 ttgctcaatc taaaaaagca cctatattat tagcatctga taatgaaata ccaaaagtaa
    10021 ctttagatta tataaaagat ataattaaga aaagcccatc agctaaaata tatatagtag
    10081 gtggagaatc agcagtatca aatacagcta aaaagcaatt agaatcagta actaagaata
    10141 ttgaaagact agctggagat gatagacata cgacttctgt agcagtagca aaagctatgg
    10201 gttcttttaa agatgcattt gtagtaggtg caaaagggga ggctgatgct atgagtatag
    10261 ctgctaaagc tgctgaactt aaggctccta taatagtaaa tggctggaat gatctttcag
    10321 cagacgctat caaattgatg gatggaaaag agattggtat agttggtggc tctaacaatg
    10381 tatctagcca aattgaaaat caacttgccg atattgataa agatagaaaa gttcaaagag
    10441 ttgaaggaga aacaagacac gatactaatg ctaaagttat agaaacatat tatggcaaat
    10501 tagataaact atatatagca aaagatggat atggaaataa tggtatgcta gtagatgcat
    10561 tggcagcagg acctctagca gcaggtaaag gtccaatact tctagctaaa actgatataa
    10621 cagactcaca aaagaatgca cttagtaaaa aattaaatct tggtgcagaa gtaactcaaa
    10681 taggtaatgg agttgaattg acagtaatac aaaagatagc taaaatacta ggttggtaat
    10741 aatttatact gtggagaaga ttccttaatt aatacaggaa tctttttcta taattatgct
    10801 atttattgta aattataaca ttttataaag taggaggatg tagtagtggt agacgcaact
    10861 ttatttttta caccacatca agatgatgaa accttaagta tgggaagtgc tataattgaa
    10921 catgttgaaa aaagtgatac acatgtcata ttatgcacag atggtagtaa atctataata
    10981 agaaaagttt tggatgatgg agggtcatgt tcttatcata ttaaagatat tcataaatac
    11041 tctttatcag aaaatgaatt ttcaaaagat agagatgaag aatttaagga tagctgtgaa
    11101 gcaatgggtg taaaagaaag taatatacat atagaagata atagagcaca tgatggtgag
    11161 ttaagtaaag aaaaggcaag ggaaataata cttaagtact tagaagaata tccagatgca
    11221 aaagtaaaaa ctgttactcc gtttaaagct agtggaatac atgaagatca tagggcatta
    11281 ggagaagctg cactagagtt atataaagaa ggcaaaataa aagatttaag attttatgta
    11341 gaaccatatg actataagga ttttaaaaaa gttaatccaa atgtagaagt atggaaggta
    11401 ttacctagtc aagaggagaa gttattaagt gcaatgaatg catataaaaa atggaatcct
    11461 gaaagtggac attatgctat aggatatcat tcagtaaaat ctcattttga tgagctagct
    11521 ataaataaaa tacagtatgt acatgctcca taaaatatta taaaagaatt aggaggtaaa
    11581 ttttaatgaa aatatcaaaa aagatagtgg cattattgac tataacattt ttaactatta
    11641 cattatatgg aaatatatct aatgcatcca caaaagacac attaacaggt tctggaagat
    11701 gggaaacagc aataaaaata agtcaagcag gatggaaaaa gtctgaaaac gctgtgctag
    11761 taaatgataa ttcaatagca gatgctttat cagctactcc atttgctaaa gcaaaagatg
    11821 cgccaatatt attaactcaa agtaataaat tggatagtag aacaaaagca gaattaaaaa
    11881 gacttggtgt gaaaaatgta tatttaatag gtggttcaat tgcattaagt tcagagattg
    11941 aaaagcagtt aaatgcagaa aatataaatt ttgaaagaat atccggaaat agtagatatg
    12001 atacttcttt aaaattagct gaaaagctag atagggaaaa atctatatct aaaatagtag
    12061 tagtaaatgg agaaaagggt cttgctgatg cagtgagtgt tggagctata gctgctcaag
    12121 aaaatatgcc aataatactt tctgattcag ataatggaac tgaagtagcc gataatttta
    12181 tagatagtaa ggatatagaa aaatcgtatg taataggtgg tacatattct atttctaatt
    12241 ctgtagaaag aagtttacca aatgcaacaa gaatagcagg tagtggtaga agtgagacaa
    12301 atgcaaaaat tatagaagaa ttttacaaag atactgatat aaaaaatatt tatgtaacaa
    12361 aagatggtac aaggaataag aatgatttaa tagattcctt agcagtaggt gtgttagcag
    12421 ctaaaaatag ttctccaata gtattatcag gaaataaatt ggatgctact caaaaggatg
    12481 tgttaaatac taagattata gataaagtta ctcaaattgg tggtttgggt aatgaaaatg
    12541 ctgtagaaga tatactagac atgcaagaag aaactaagta tactgttgaa actattgatg
    12601 aacttaatgt tgctataaag aaagcagatg caaatgatac tataaaattt atacctgaaa
    12661 aagataagaa cataaatagt tcatttaaaa tagaaactga caaggctata aaagttgagt
    12721 ttgatggagt gtacagacaa tcaataacta taaatatgcc taatggtgat gttgataatt
    12781 taggtgaaat taatggcact atagaaataa gaaacataaa gaataattca ttagtaaata
    12841 gaggtagtat acaagattta gatatttcta ctgaaaatgg atgtaaaata gaaaatgaaa
    12901 gttctggtga aatttggctt ataactatat tatcaaatgc aaaaggagtt tatattataa
    12961 ataatggcga tataacaaaa atatcaaata gttcctctga tgttataata aaaaactcag
    13021 gagatataga tattataact ggtagtgaag agccagcaat aagtgggaga aagcctaaag
    13081 ttaatgacac tgatatagat gataataaac ttgcaagagg actttctgtc aagacagaat
    13141 cttgttatgt accagaagaa gattatatta tggttactgt attaagtgaa cctagaaatt
    13201 tagattataa tatatattat agagttgtag ataataagcc ttcagctatg tctgtaggag
    13261 aaaaaattag tcctaaaaat tgggatgctg ttccaaaatt taataaagca tttcttgaga
    13321 aagctaaaga tggttcatat atagaagttg ttgagataaa ttcatcaaca aataaagttt
    13381 caagatgggg cagatctaat gcaactgatg atgattttag ataatatata tttaagtaga
    13441 caatttttta ttgtctactt tttatttttt aaaaattatc atcttgctaa acaagtaatt
    13501 taaaactata acaactatat tagtaaatac ttttacaatg acatcattta caaaaagcaa
    13561 atcaatcatt acatacataa ttaacatttc aacgacacca gaaagtagcc taaaactaac
    13621 aaaagacaaa aattccttaa ataagaactt tatttcaatt cttttactct caaacacaaa
    13681 aaacttatta gtaacataag caaataaaac agcaagtatc catgccaaag catttgcaac
    13741 cataaaatta aataaaataa ctctagtaaa aaataaatac gaaactatat ttacaagagt
    13801 agtaaatgct ccaaaaaata aatataatat tgtctctcta tgctttttta aaatcaaata
    13861 taatcctcct aagctaattt aatataaata tacttaaaga tactaacaaa cattagtata
    13921 gcacatctat taataaatca atatatctta aaatattata aaaataaaac tataatattc
    13981 tataattaat atgtactcat aatccatatt ttatcaaaat aaaatatgac cttaatttag
    14041 aaatctgata taataaaaat taaatacata taagggggta aacatgagaa agtataaatc
    14101 aaaaaaattg tcaaagctac tagcattgtt gacagtttgc tttttaatag tgtcaacaat
    14161 acctgtctca gcagaaaacc ataaaactct agatggagta gaaactgcag agtattctga
    14221 aagctatctt caatacctag aagatgtcaa aaatggagac acagcaaaat ataatggagt
    14281 aatacctttt ccacatgaaa tggaaggtac aacacttcga aataagggca gaagtagtct
    14341 tccatcagca tataaatcaa gtgtagcata caacccaatg gatcttggtc ttactacacc
    14401 agcaaaaaat caaggaagtc ttaatacatg ctggtctttt tcaggtatgt caactttaga
    14461 agcatatctt aaattaaagg gatatggaac gtatgactta tcagaagaac atttaagatg
    14521 gtgggcaact ggtggaaaat atggatggaa cttagatgat atgtcaggga gttcaaatgt
    14581 aacagctata ggatatctga cagcatgggc aggtcctaag ttagaaaagg acataccata
    14641 taatcttaaa tctgaggcac aaggtgcaac taaaccttca aatatggata ctgcacctac
    14701 tcaatttaat gtgacagatg ttgttcgtct taataaagat aaggaaactg taaagaatgc
    14761 tataatgcaa tatggttcag tgacatcagg ttatgcacat tattcaacat attttaataa
    14821 agatgaaaca gcatataact gtacaaataa gagagctcca ttaaatcacg ctgtagcgat
    14881 agtaggatgg gatgataatt attcaaaaga taactttgca tctgatgtaa aaccagaatc
    14941 aaatggagca tggttagtaa aaagcagttg gggagagttc aattctatga aaggattctt
    15001 ctggatttct tatgaagata aaactctact aacagataca gataactacg caatgaaatc
    15061 agtatcaaaa ccagatagcg ataaaaaaat gtaccaactt gaatatgctg gtcttagcaa
    15121 gataatgtca aataaagtaa cagcagcaaa tgtatttgat tttagcagag attctgaaaa
    15181 acttgactct gttatgtttg aaacagactc tgtaggagca aaatatgaag tatattatgc
    15241 accagtagta aatggagttc ctcaaaacaa ttcaatgaca aaacttgcaa gtggaacagt
    15301 atcatattct ggatacataa atgtacctac taattcttac agtttaccaa aaggtaaagg
    15361 agcaatagta gtagttatag acaacacagc aaatcctaat agagaaaaat caactttagc
    15421 atatgaaact aatatagatg gatactatct atatgaggct aaagcaaact taggtgaaag
    15481 ctacatactt caaaacaata agtttgaaga tataaataca tatagtgaat tttctccttg
    15541 taattttgtt ataaaagcta taataaaaac atcttctgga caagctactt caggagaatc
    15601 tttaactgga gcagatagat atgaaacggc agttaaagtt agccaaaaag gatggacttc
    15661 ttcacaaaat gcagtattag taaatggaga cgcaatagta gatgctttaa cagctacacc
    15721 atttacagca gcaatcgact ctccaattct tttaacagga aaagataact tagattcaaa
    15781 aactaaggcg gagttacaaa gattaggaac taaaaaagtt tatctaatag gtggagaaga
    15841 ctctttaagc aagaatgtac aaactcaact tagcaatatg ggtatatcag tagaaagaat
    15901 tgcaggtagt gatagataca agactagtat atctctagct caaaagttaa atagtataaa
    15961 atctgtttca caagttgcag tggcaaatgg tgtaaatgga cttgcagatg caataagtgt
    16021 tggtgcagca gctgctgata acaatatgcc aataatactt actaatgaaa agagtgagtt
    16081 gcaaggtgct gatgagtttt taaattcatc aaaaataact aagtcttata taattggtgg
    16141 aacagctact ttatcatcaa atttagaaag taagctttca aatccaacaa gacttgcagg
    16201 aagtaataga aatgaaacta atgctaagat aatagataaa ttctacccta gctcagattt
    16261 gaaatatgct tttgttgtta aagatggttc aaagagtcaa ggagacttga tagatggact
    16321 agcagtagga gcattaggtg ctaaaactga ttcaccggta gttttagttg gaaataagtt
    16381 agatgaaagt caaaaaaatg tacttaagtc taagaaaata gaaactccta ttagagttgg
    16441 tggaaatgga aatgaaagtg cttttaatga actaaatact cttttaggaa aatagtgaat
    16501 ggaaagatag taaggatata cattttatta ttaaaaaata ttcaatagaa gtttataaat
    16561 ataaaataac aaaacggggt gtctcaaaat gaacttttta gttcatgagg cactcttttt
    16621 tgttgtttta tatttttatc atttaaataa tgaaaaatag gcttgtctat atttttagat
    16681 agttttctta ttattgatga aaaatgtaat aattttaaac tgtaaaatag tgtaaagtta
    16741 ttttataata taaatatagt aagattttaa acaaaataag ggggaataat aatgaaagca
    16801 ccaaaaacta ttttaacaat actaacaata gcgcttactt taagtagcat ttctataata
    16861 ccatcatatg cacttacaga ggaaaaatta ataggtaatg gtagatatga aactgctgta
    16921 aagataagtc aaaaggcata cagttctagt tctaatgtag tgttagttaa tgacaattca
    16981 ttagctgatg cactttcagc tacaccattt gcaaaggcta aaggagcacc aatacttttg
    17041 acagaaagtg ataagctaga tgataggact gaaaaagaga taaaacgtct aggagctaaa
    17101 gatatttatt tgataggagg tactgctgta cttaataaag atatagagaa caagttaaaa
    17161 ggtaatggac ttaatgtaga aagaattaat ggtaaggata gatatgaaac ttcactgatt
    17221 ttggctaata agcttaagga tataaaagat attaaggaag ttgctgtagt aaatggtgaa
    17281 aaaggattaa gtgatgctgt tagtgttgga gcaccagcag ctcaaaataa gatgcctata
    17341 attttgtcta gtcctaagga tggagtagaa gcatttgata aatttataag ggatgagaaa
    17401 gttataaaag catatgtaat tggaggtaca acttcagttt caagagctgt agaaaagagt
    17461 cttcctaatg cagaaagagt gagtgggaaa gatagaaatg agactaatgc taaagttata
    17521 gaaaagtttt acactgatac aaatttaagt aatttatatg taactaagga tggtagtaaa
    17581 aatgagaatc aactaataga ctcgttagct gttggagtat tggctgcaaa aaatgagtct
    17641 cctgttgttt tggttggtaa taaattaaac acaaaacaaa gagatatact tagtactaaa
    17701 aaattaaata ctataacaca ggtaggtgga aatggaaatg aagaagcttt tgatgaaatt
    17761 aaaagtctac aagaaaaaac tgtgtttgaa gcaaaaactg ttgaggaatt aactgatata
    17821 ataaatatag caagtcctaa tgatattata aactttaaac caaaagagaa tactgtaaat
    17881 gaagctttta gaatggtaac aaataaacca ataactgtga atataaaggg tgattgttca
    17941 aagactctta cagtagatat gcctaatggt gaagtgaata actatgctac tttggtaaat
    18001 gttatagtta gaaatattgg agaaggtgga tttaataatc atgatacaat cactatattg
    18061 tcagttcgtg acaaaaatgg tagagttata gaaaatacta gaaattcaga tatagatacg
    18121 ttgatgatat tagcttctgc taatgataca aaattgatta atgatggata tataggaaaa
    18181 cttatagata attcatctaa tagtgatata actaataatg gtactataga taaaaaagtt
    18241 aatcaggttg aagatttaga ggctaaagta gattcaattg aaaaagctat agattctata
    18301 agtcaaaaag tcaataaaat acaagatata ctagataagc ttggatttct aaagaaattt
    18361 cttagctaag aatatgtata tttaagtgaa gtgtgtgttg taattgttct aaagttatat
    18421 agttaaacag gtttatatac aaattgattt atagataaat gtgtagttta gtatatattt
    18481 gaataagctt tggggaggta acatggggat tggaattgaa tgtaacaaaa aaataaataa
    18541 aagtattgac aaaactagaa taaagaaaaa taatct
//
LOCUS       DBGJFF010000074        18576 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.075, whole genome shotgun sequence.
ACCESSION   DBGJFF010000074 DBGJFF010000000
VERSION     DBGJFF010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18576
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.075"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            <1..321
                     /locus_tag="ACOIAF_002482"
     CDS             <1..321
                     /locus_tag="ACOIAF_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HGZ2669489.1"
                     /translation="VEKADCYTGNTIESERFFPGWLYDEEDEFVQAAYKGLKEAGIDS
                     EITQYSFCTNGSHYAGEAGIRTIGFGPSKENLAHTIDEYIELDQLFKGAEGYYGILKS
                     VYKK"
     gene            337..1920
                     /locus_tag="ACOIAF_002483"
     CDS             337..1920
                     /locus_tag="ACOIAF_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HGZ2669490.1"
                     /translation="MRTLIKHGLIVDGNKTPAYEGDILIENEKILKISQDLNEEADKV
                     IDAKGRVICPGFIDTHSHSDLVILVNPYNEVKIRQGITTEVLGQDGISMAPLPQEHIS
                     SWRKNLAGLDGESDEIDWKYETTENYLKMMDYNGVGLNETYLVPHGNVRMEAMGLEDR
                     PATKEEIQKMCEITERELKAGAIGLSTGLIYIPCAYSLTEEIIEMCKVVAKYDGVFVV
                     HQRSEADTILTSMEEIIEIGKQSGVKVHFSHFKVCGKANWKYIPQVIELLEKAEKEGI
                     RVSFDQYPYAAGSTMLGVVLPPWAHSGGTDKLIERLSDENERAKMKKDIANGIEGWDN
                     FIEFAGIDQIFVTSVKTEKNKDTIGKSLLEIGKMRGKDPLDATFDLLKEEENAVGMVD
                     FYGLEEHIIGFMKRDEQNVCTDGLLAGKPHPRAYGSFPKILGRYVRELNVLTIEEAVY
                     KMTKKAATSFSIKDRGELKEGYFADIVIFDKDTVSGCDDFINSMQYPTGIDYVIINGN
                     CVVEEGKYNKIKAGKVLKN"
     gene            1948..3276
                     /gene="ssnA"
                     /locus_tag="ACOIAF_002484"
     CDS             1948..3276
                     /gene="ssnA"
                     /locus_tag="ACOIAF_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HGZ2669491.1"
                     /translation="MILVGNGRLISQDSLNPYMEDGCVVINDNLIEDIGTTEEMKAKY
                     PVHEFIDAGKKIIMPGLINSHMHIYSSFARGMAVPGKPSENFLEILNNLWWRLDKQLT
                     LEDTKYSAYATYIECIKNGVTTVYDHHASPNAIEGSLFTISDVAKDLGIRTCLCYEVS
                     DRDGGNTIQQGINENVNFIKYAQKDDTDMIKGMFGLHAAFTLSDDTLNKCASEMAGLD
                     TGYHVHVAEGLADLQYNVDKYGKRVLERFNDFGVLKEKTIAVHCIYISAEEISLLNKT
                     NTFVVHNPESNMGNAVGCSPAIELLKRGVTVGLGTDGYTSDMFESLKVANIIHKHHLK
                     DPIVGFEESQTMLFDNNRKIAKAYYKNDLGVLKKGAYADVIVVDYIPHTPLTENTIGG
                     HTIFGMSGRSVDTTIINGKVLMKDRKLINIDEESILAKSRELSQNLWNRI"
     gene            3421..4773
                     /locus_tag="ACOIAF_002485"
     CDS             3421..4773
                     /locus_tag="ACOIAF_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HGZ2669492.1"
                     /translation="MSRDKSPKIAPVDERLPVSKSWVFSLQHVLAMCAGAVAVPMMIG
                     SAARLDHASIVFLINACLFMAGIGTLIQSVGIGNIVGAKIPVIEGTSFAAVAGMTAIA
                     SSYSDSQVAIQTVFGSVIVAGLFCILLAPVFEKLIRFFPSVVTGTVVMVIGISLFPVG
                     IKWITDNKLEAVEPNSLALALAVLAITLLSFKFLKGIWNSTAILVGIVLGTLLSMAVG
                     MADFSAVNGVGWLNLNVPLKFGMPRFEPSAILSLCLIMLVLMTESVGNMIAIHEMVDK
                     EVTGKNIKRGLFGDGISTALSGIFNSFPITPFGQNTGIVGLTGIKSRFIAVYAGVILL
                     VLGFLPKFAAIIGAIPKPVLGGVGFAMFGMVMVGGVKTLSKVKFDGTKNGVIVAVSVG
                     LSMIPLTNPDFYHNFPTWVQTIFHSGITTGSLSAVLLNIFFNELGSKKEKNNKVEEAK
                     "
     gene            4965..6074
                     /locus_tag="ACOIAF_002486"
     CDS             4965..6074
                     /locus_tag="ACOIAF_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HGZ2669493.1"
                     /translation="MANLKVNLYGMEFLNPIMTAAGPGAKDGALCQEAAKGGAGAICT
                     KTISVLPADVPRPCMAKTNSGFLNTELWSELPKEQWIEEEYKKVKETGLPVIVSMGYS
                     AEQIAEVAPLVKPYADAVELSTHYVGTDVAPIVNALKAAKAVLDVPVFMKMSPHTDIQ
                     TIAKAVEEAGADGLVMINSFGPCMAIDVNTGYPIMGSKAGYGWLSGAPIRPLAIRCIY
                     EASQVVNIPIIGVGGITCGKDVAEMMMAGASAVQVCTEAILKGPSIYGKIAKELNEFL
                     DENGYADVNEIKGLTHKKMAARGFRTHSVAPVVDDNKCIKCKQCVTSCVYEALEVTDK
                     LNIDADKCFGCGLCVTRCPKGALSICYNQVEAMNE"
     gene            6357..7748
                     /gene="hydA"
                     /locus_tag="ACOIAF_002487"
     CDS             6357..7748
                     /gene="hydA"
                     /locus_tag="ACOIAF_002487"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HGZ2669494.1"
                     /translation="MGIVLKGGKVVTSEKTYCADVRIEDEKIVDIGLDISKENDEIVD
                     VSDSYVIPGGIDTHTHFDLDTGVTKTADNFETGTKAAIVGGTTTILDFATQAKGCSLK
                     DGLEEWHEKAKDKSYSDYGFHMAITDWNDSVCNEMEDMVKEGVSSFKLYMAYKGSLQV
                     DDGVIFEALRKAEEIGGIIGFHCENGDIICELVDKAKSENNLSPKYHQLTRPAIMEAE
                     ATSRLMKIAEVANSKVYVVHLSCKESLEEVLKAKQRGVDVIVETCPQYLLLNDELYDL
                     DGFESAKYVMSPPLRNKENNEILWNAILDNQIDTIGTDHCSFNYKGQKELGINDFSKI
                     PNGSPGVEHRMSLLYTYGVCKNKISINKMVEVTSTNAAKLFGMYPNKGTIEIGSDADI
                     LVLNPNKKSKIEAKKQLQNVDYTPYEGYEVDCQIEHVYLRGKEVVRDGELICNTPSGR
                     YINRRLTKGAREW"
     gene            7742..10303
                     /gene="xdh"
                     /locus_tag="ACOIAF_002488"
     CDS             7742..10303
                     /gene="xdh"
                     /locus_tag="ACOIAF_002488"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0046415 - urate metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent xanthine dehydrogenase"
                     /protein_id="HGZ2669495.1"
                     /translation="MVEFILNGEKVVAKEGKKLLDFLREDMNITSVKNGCSEGACGTC
                     MVIIDGKATKACVFKTDKLAGKNITTIEGLSERERDVFAYSFASQGAVQCGFCIPGMV
                     ISSKALIDVNNNPTEDDIKKALKGNICRCTGYVKIIKAVDLAAKIFRENIDVPKVNCK
                     GLVGENLPRVDAVGKALGYSEYVDDMRIEGMIYGSAVRAKYPRALVKKIDIEEAKAYP
                     GVVAVLTAKDIPGTIKVGHLKKDWDTLIPEGEITRYLGDGIVLVAAETREALEEAKKL
                     VKIDYEELTPLSTTADALREDAPKIHETGNILVKEHLVRGNAKEKIENSKYVVTKRYT
                     TPATEHAFLEPECAVAGPYEDDGVIIYSTDQGVFATQHECADMLGLPYEKVRVINKMV
                     GGGFGGKEDMSVQHHAALLAYHTKRFVKVQLTRKESIIVHPKRHATEMEFTTACDENG
                     YLTGMKATIISDTGAYASLGGPVLQRLCTHAAGPYNYQDIDITGTAVYTNNPPGGAFR
                     GFGVTQSCFATECNLNLLAEMVGISPWEIRYKNAIRPGQVLPNGQFADEGTAMAETLE
                     AVKEEYEKHPRAGIACAMKNSGVGVGIPDVGRCRLVIKDGKVHIRTSASCIGQGLGTV
                     MTQVVCETANLLPENIVHEVPDTHLTPDSGNTTASRQTVFTGEATKVASNKLKEALDS
                     GKTLLDLEGEEFYGEYESITDKMGCDKENPVSHVAYGYATQVVILDENGKLEKVVAAH
                     DVGRAINPISLEGQIEGGVVMGLGYALTEDYPVVNSVPTAKFGTLGLFKSTDIPEIQA
                     LIVEKNKDLLAYGAKGVGEICTVPTAPAVQNAYYVFDKEFRTELPLQNTPYSKKK"
     gene            10455..11801
                     /gene="rpoN"
                     /locus_tag="ACOIAF_002489"
     CDS             10455..11801
                     /gene="rpoN"
                     /locus_tag="ACOIAF_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HGZ2669496.1"
                     /translation="MNFNNSLELTQSQKLIMTTQLKQSLSILNMSKLELEEEIKREAE
                     NNPLVEVEKSGEINWEEYIKDMDRSRRLDRTEISYNPDNEVNLENLVKYSSNLYEDLK
                     FQISLYKLTDKELEVCEYIIDSLDEDGYLRTEEKVIVDTLKIDEELFEKCLISVQQLE
                     PSGVGARSLSECLMIQMASLGIYNEILEEIVTKDLNLIANNKYKEISKKYNMSLQKCV
                     ELINIIKTLDPKPGRTCSVEKSVYIQPDVTVEKIDDEFIVYLNEKDSYQIRINSYYKD
                     ILKNSQSDESAKEFIKERLNSATGLMKNIESRKTTVLKIAEEIVKAQDEFLRKGTKYI
                     KPLKMKDIAEKLEFHESTISRGVNGKYMLTPFGVYEFRYFFSSAIETENNEMASSTSI
                     KKIIKETIKDENKKKPLSDDHISKLLKEKGINVARRTVAKYREELGIPSSSKRKEY"
     gene            12599..13621
                     /locus_tag="ACOIAF_002490"
     CDS             12599..13621
                     /locus_tag="ACOIAF_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HGZ2669497.1"
                     /translation="MKNLLEIQKKLIPQAIEFMERRYSILRQISLSEPIGRRTLSNVL
                     EISERIIRSETEFLKEQGLIDVAVSGMTITKEGSDLLDKLKDIMSDIMGLSKLQDRVR
                     EKLGIKRVILVPGSCDENGSLLKDVTRYGCEYFLGILKDGDIVSITGGSTMLEFSNVI
                     KTDKRYPNSTIVPARGSMGTDVEMQSNNIVASISKKLHSNCKLLHIPDELDESAMKTL
                     SQVPEIKTTLEYIKRTDILVFSIGRADVMAKRRKLPEDKVDEILSKNAVGEAFGYYFN
                     KDGEIVYKLNTVGIDLETVKQVKETIAIFAGTEKVEALLAISNMHKNMVLVTDEESAY
                     KILSLL"
     gene            13681..14688
                     /gene="gap"
                     /locus_tag="ACOIAF_002491"
     CDS             13681..14688
                     /gene="gap"
                     /locus_tag="ACOIAF_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HGZ2669498.1"
                     /translation="MVKVAINGFGRIGRLALRKMMEQQDKFEVVAINDLTDAKMLAHL
                     FKYDTAQGRFNGEIEVKEGAFVVNGKEIKVTAERNPADLPWAELGVDIVLECTGFFTS
                     KDKAEAHIQAGAKKVVISAPATGDLKTIVFNTNSDILDGSETVISGASCTTNCLAPMA
                     KVLNDKYGIEKGLMTTIHAYTNDQNTLDGPHPKGDLRRARAAAGNIVPNTTGAAKAIG
                     LVIPSLKGKLDGAAQRVPVVTGSITELVCTLGKNVTVEEINAAMKEASNESFGYTEEM
                     LVSSDIIGISYGSLFDATQTKVMEVDGKQLVKVVSWYDNEMSYTSQLIRTLGYFAQLA
                     K"
     gene            14910..16112
                     /gene="pgk"
                     /locus_tag="ACOIAF_002492"
     CDS             14910..16112
                     /gene="pgk"
                     /locus_tag="ACOIAF_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HGZ2669499.1"
                     /translation="MSMLNKKTIEDIDVCGKKVLVRCDFNVPLQDGVITDENRLNGAL
                     PTIQYLISKGAKVILCSHLGKPKGEAKPELSLAPVAKRLSEMLGKEVVFAADDNVVGE
                     NAKKATEKMENGDVVLLENTRYRKEETKNEENFSKELASLAEIFVNDAFGTAHRAHCS
                     TVGAGEFLQERVCGYLIQKELKFLGEAVANPVRPFTAILGGAKVSDKLAVINELLEKV
                     DNLIIGGGMAYTFLKAQGYEVGTSLLEIDKVEYAKEMMEKAKNKGVNLLLPVDVVMAD
                     HFAPDATPIVTEDANVKEDYMGLDMGPKTIANFVKTIKESKTVVWNGPMGVFEFENFA
                     NGTLSVARAMAELTDATTVIGGGDSAAAVNQLGFGDKMTHVSTGGGASLEFLEGKELP
                     GIAALDNK"
     gene            16138..16881
                     /gene="tpiA"
                     /locus_tag="ACOIAF_002493"
     CDS             16138..16881
                     /gene="tpiA"
                     /locus_tag="ACOIAF_002493"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HGZ2669500.1"
                     /translation="MRKPIIAGNWKMHKTIKEALEFVNEVKDKVNSDKVEAVICAPFT
                     LLKDLKEATKGTNIKIGAQNMHFEEKGAFTGEVSPLMLKEIDMDYVVIGHSERRQYFN
                     ETDETVNKKVLKALEVGIDPILCVGETLEQREAGKTKDVCKVQVEKALENVLKDDLAK
                     VVVAYEPIWAIGTGKTATAEDANDVISYIREVIKGLYGELANEVRIQYGGSVKPSNVA
                     EIMGQSDIDGALVGGASLASNDYLDLVNF"
     gene            16893..18422
                     /gene="gpmI"
                     /locus_tag="ACOIAF_002494"
     CDS             16893..18422
                     /gene="gpmI"
                     /locus_tag="ACOIAF_002494"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HGZ2669501.1"
                     /translation="MKKPVALIIMDGFGYNKDVKGNAIAESKTPNLDRIKKEYPNTLI
                     NASGLDVGLPDGQMGNSEVGHTNIGAGRIVYQDLTRITKSIKDGDFFTNKVLCEAMDN
                     AKENSLHVMGLLSDGGVHSHIDHLKAIIKMAKDKGVQKVYVHAFTDGRDTDPQSALEY
                     AKEVQVSMDEIGVGEFATVSGRYYAMDRDKRWERVELAYNAMVRGIGEKANSIEEAIQ
                     NSYDDGKNDEFIMPTVIMKDDKPVGSIKENDSIIFFNFRPDRARQITRALVCEEFDGF
                     KREDIKNFFVCLTEYDITIENVHIAFGPQSLANTLGEYLAKNGKTQLRAAETEKYAHV
                     TFFFNGGVEEPNKGEERLLIPSPKVATYDLKPEMSAYELTDKALDKLGEDKFDFIVLN
                     FANPDMVGHTGSIEAAIKAVETVDTCVGKLIDKIVELGGSAIITADHGNAEYMLDPET
                     GKTVTAHSINPVPFIVVGQEYESAKLLDGGRLSDIAPTVLDMMKLEKPEEMTGHSLIS
                     K"
BASE COUNT         7431 a         2143 c         3751 g         5251 t
ORIGIN      
        1 gttgaaaaag ctgattgcta tacaggaaat actatagaaa gtgaaagatt cttcccaggt
       61 tggttatatg atgaagaaga tgaatttgtt caagcagcgt ataagggatt aaaagaagct
      121 ggaatagatt cagaaataac tcaatactca ttctgtacga atggaagtca ctacgcaggt
      181 gaggctggaa taagaactat aggatttggt ccttctaagg aaaatcttgc tcatacaata
      241 gatgagtata tagaattaga ccaattattc aaaggtgcag aaggatatta tggtatatta
      301 aaatcagttt ataaaaaata attaggagat ggttttgtga gaactttaat aaaacatggg
      361 cttatagtag atggaaataa aacaccagca tatgaaggag atattttaat agaaaacgag
      421 aaaatattaa aaatatcaca agatttaaac gaagaagctg acaaagttat agatgcaaaa
      481 ggtagagtta tatgtccagg tttcatagat actcatagcc attcagacct agttatttta
      541 gttaatccat ataatgaggt aaagattaga cagggaatta caacagaagt gttgggccaa
      601 gatggtatat ctatggcccc tctacctcaa gagcatatat cttcttggag aaaaaattta
      661 gcaggtctag atggtgaaag tgatgaaatt gattggaaat atgaaacaac agagaattat
      721 ttaaaaatga tggattacaa tggggtaggt ttaaatgaaa cttatttagt acctcatgga
      781 aacgttagaa tggaagctat gggacttgaa gatagacctg ctactaaaga agaaattcaa
      841 aaaatgtgtg aaatcacaga gagagaatta aaagcaggag ctataggatt gtctactgga
      901 cttatatata taccttgtgc atactcattg actgaagaaa ttatagagat gtgtaaggta
      961 gtagcaaaat atgacggagt atttgtagtt caccaaagaa gtgaagcaga tacaatttta
     1021 acttcaatgg aagaaatcat agaaattgga aaacaatcag gagtaaaagt tcatttttca
     1081 cattttaagg tttgtggtaa agcaaactgg aagtatatac cacaagttat agagctttta
     1141 gaaaaagcag aaaaggaagg aataagagta tcttttgacc aatatccata tgcagcagga
     1201 agtacaatgc ttggagtagt tttacctcct tgggcacatt ctggaggaac tgataagtta
     1261 atagaaagat taagtgatga aaacgaaaga gcaaaaatga aaaaagatat agcaaatgga
     1321 atagaaggct gggataattt tatagaattt gctggaatag accaaatatt tgtaacaagc
     1381 gtaaaaacag aaaagaataa agatacaatt ggtaagagct tattagaaat tggaaaaatg
     1441 agaggaaaag acccattaga tgcaacgttt gatttattaa aagaagaaga aaatgcagtt
     1501 gggatggtag atttctatgg attagaagaa catataatag gattcatgaa aagagacgag
     1561 caaaatgtgt gtacagatgg tcttttagca ggtaaaccac atccaagagc ttatggttct
     1621 ttccctaaaa tactaggaag atatgttaga gagttgaatg tgttaacaat cgaagaagct
     1681 gtttataaaa tgactaaaaa ggcagctaca agctttagta taaaagatag aggcgaatta
     1741 aaagaaggat attttgcaga tatagttata tttgacaagg atacagttag tggttgtgat
     1801 gattttatca actctatgca gtaccctaca ggtatagatt acgtaattat aaatggaaat
     1861 tgtgttgttg aagaaggaaa atacaataaa attaaagcag gaaaagtact taaaaattaa
     1921 atcttagctt taaggaggaa taaatttatg attttagtgg ggaatgggag gctaataagc
     1981 caagattctt taaatcccta tatggaagat ggatgtgtag taataaatga taatctaatt
     2041 gaagatatag gtactacaga agaaatgaaa gctaaatatc cagtacatga atttatagat
     2101 gcaggcaaga aaataatcat gccgggtcta ataaattcac atatgcatat atacagctca
     2161 tttgctagag gaatggcagt tccaggaaaa ccttctgaga actttttaga gatactaaac
     2221 aatttatggt ggagacttga caaacagctt acactggaag atacaaaata cagtgcatat
     2281 gcaacgtata tagaatgtat aaaaaatggt gtaacaacag tttatgacca tcatgcaagt
     2341 ccaaatgcaa ttgaaggcag tttgtttaca atatcagatg ttgcaaagga tttgggaatt
     2401 agaacgtgtc tttgttatga agtgtctgat agagatggtg gaaatacaat ccaacaaggt
     2461 atcaatgaaa acgtaaactt tataaaatac gctcaaaaag atgatacaga catgataaaa
     2521 ggaatgtttg ggcttcatgc tgcatttaca ctatctgatg atacattaaa taaatgtgct
     2581 tcagagatgg ctggtcttga tacaggttac catgtacatg ttgctgaagg actagcagac
     2641 ttacaatata atgtagataa atatggaaaa agggttttag aaagattcaa tgattttgga
     2701 gtactaaaag aaaaaacaat agcagttcat tgtatatata taagtgcaga agaaatcagt
     2761 cttttaaata aaacaaatac atttgtagtt cataatccag aatctaatat gggaaatgca
     2821 gttggatgtt ctcctgcaat agagttatta aaaaggggag ttacagtagg attaggaact
     2881 gatggatata ctagcgatat gtttgaatca ttaaaagttg ccaatataat tcacaaacat
     2941 cacttaaaag acccaatagt tggatttgaa gaatcacaaa caatgttatt tgataacaat
     3001 agaaaaatag ctaaagctta ttataaaaat gacctaggag tacttaaaaa aggtgcttat
     3061 gcagatgtta tagtagttga ttatatacca catactccat tgacagaaaa tacaataggg
     3121 ggacacacta tttttggaat gagtggaaga agcgtagata caactattat aaatggaaag
     3181 gtacttatga aagatagaaa gttaataaac atagatgaag aaagtatttt agcaaaaagt
     3241 agagaattaa gtcaaaatct atggaataga atttaaataa ttgatattaa tatacaagta
     3301 agaaacaaga atttagatgt taaaaacaga gcttaaacaa aaataggaat ttaaatgaag
     3361 tatataaata aaaaatatac ggaatagaat attaaattaa atatagaagg gagacccaaa
     3421 atgtctcgag ataaaagccc taaaatagct ccagtagatg aaaggttacc tgtatccaaa
     3481 tcttgggtat tttcactaca gcacgtttta gcaatgtgtg caggagcagt agcagttcct
     3541 atgatgatag gaagtgctgc acgactagat catgctagta ttgttttttt aataaatgct
     3601 tgcttgttta tggcaggtat aggaacactc atacaaagtg ttggtatagg aaatattgta
     3661 ggtgctaaaa taccagtaat agaaggaact agttttgccg ctgtggcagg tatgacagca
     3721 atagcctcat catatagtga ttcacaagtt gcaatacaga cagtatttgg ctctgtaata
     3781 gtagctggat tgttttgtat attacttgct ccagtatttg aaaaattaat aagatttttc
     3841 cctagtgtgg ttactggaac agttgttatg gtaataggta tttcactatt tccagttggg
     3901 ataaaatgga taactgacaa caaattggaa gcggttgaac caaactcatt agcattagct
     3961 ttagcagtat tggcaataac tttactttca tttaaattct taaaaggaat ttggaatagt
     4021 acagctatat tggtaggtat agtacttggt acactacttt ccatggcagt tggaatggca
     4081 gattttagtg cagtaaatgg agttggttgg cttaacttaa atgtaccttt aaaatttgga
     4141 atgcctagat ttgaaccatc tgcaatacta tcattgtgtt tgattatgtt ggtacttatg
     4201 acggaatcag ttggtaatat gatagctatt catgaaatgg tagacaaaga agtaacaggt
     4261 aaaaatatta aaagaggtct atttggagat ggaatatcta cagcattatc tggaatattc
     4321 aattcatttc caataactcc atttggtcaa aatacaggaa tagtaggtct tacaggtata
     4381 aaatcaagat ttatagcagt ttacgcaggg gttatattgc tagtacttgg attcttacca
     4441 aaatttgcag caataatagg agcaatacct aagcctgttc ttggaggcgt aggatttgca
     4501 atgtttggaa tggtaatggt tggaggagta aaaactttaa gcaaagtgaa atttgatgga
     4561 actaaaaatg gggttatagt agcagttagt gttggtttgt ctatgatacc actaacaaat
     4621 cctgatttct atcataattt cccaacttgg gtacaaacaa tattccatag tggaataaca
     4681 acaggtagtt tatcagcagt gcttttaaac atattcttca atgaactagg ttctaaaaaa
     4741 gaaaaaaata ataaagttga agaagcaaaa taaaaataaa acaagtaaaa atctatattg
     4801 acttaaagtc attaagcatt aataaaaata attcagagat attaataatt caacaaaact
     4861 atcttatatt ttaaataata taaaaaagta aataaataac gaatacaaat aaaagaatac
     4921 aatctaaata ttaaaaatat ttaatttaag ggagagataa taatatggct aatttaaaag
     4981 ttaatttata tggaatggaa tttttaaacc caattatgac agcggcagga cctggagcaa
     5041 aagatggtgc tctctgtcaa gaggcagcaa aaggtggagc aggagctata tgtacaaaaa
     5101 ctatatctgt attaccagct gatgtaccaa gaccatgtat ggcaaaaact aatagtggtt
     5161 ttttaaacac agaattatgg tcagagcttc caaaagagca atggatagag gaagaataca
     5221 aaaaggtaaa agaaacaggg cttccagtaa tagtaagtat gggatattca gcagagcaaa
     5281 tagctgaggt tgctccttta gtaaagcctt atgcagatgc agttgaatta tctactcatt
     5341 atgtaggtac agacgttgca ccaatagtaa atgctttaaa agctgcaaaa gctgttttag
     5401 atgtaccagt atttatgaaa atgagtcctc atactgacat tcaaactatt gcaaaagctg
     5461 ttgaagaagc aggtgcagat ggacttgtaa tgataaactc atttggacca tgtatggcaa
     5521 tagatgtaaa tacaggatac cctataatgg gaagtaaagc tggatacgga tggttatcag
     5581 gtgcaccaat aagacctcta gcaataagat gtatatatga agcttctcaa gttgtaaata
     5641 taccaataat cggtgttggt ggaatcactt gtggaaaaga cgtggctgaa atgatgatgg
     5701 ctggagcaag tgctgttcaa gtatgtacag aagcaatcct taaagggcct tctatctatg
     5761 gaaaaattgc taaagaatta aatgaatttt tagatgagaa tggatatgct gatgttaatg
     5821 aaataaaagg attaactcat aagaaaatgg cagctagagg gtttagaact cattcagtag
     5881 ctccagtagt ggatgataac aagtgtatca aatgtaagca atgtgtaaca agctgtgtat
     5941 acgaagcttt agaagttaca gataagttga acatagatgc agacaagtgt tttggatgtg
     6001 gattatgtgt aactagatgt ccaaaaggag ctttaagcat ctgctacaat caagtagaag
     6061 ctatgaatga gtaataatta tatagaattt tggtaattaa cttagagtaa taggtattag
     6121 gataataaaa tatatcagat aaacaaacac acattatgca ttaataatac agtctaaagt
     6181 taataatttt attatattga tttaactaaa tattagttta gctgaatatt aatttaatta
     6241 ggtattggtt taatcttaat atagatttac tacgtataag tgaactaact agattacttt
     6301 aagttaattt tttcatataa aataaattga tacattacaa gtaaggtgga gtctatatgg
     6361 gaattgtttt aaaaggtggg aaagtagtaa caagtgaaaa aacttactgt gcagatgtaa
     6421 gaatagaaga tgaaaaaata gtcgatatag gtcttgatat atcaaaagaa aatgatgaaa
     6481 tagttgatgt tagtgattct tatgttatac caggaggtat agatacacac acacactttg
     6541 acttagatac aggcgttact aagacagcag ataactttga gacaggaaca aaagctgcta
     6601 tagtaggagg aactactact atattggatt ttgctacaca agctaaagga tgttctttga
     6661 aagacggatt agaagaatgg catgaaaaag ctaaggataa atcctattca gattatggct
     6721 ttcatatggc tataacagat tggaatgact cggtttgtaa tgaaatggag gatatggtga
     6781 aagagggagt atcttcattt aaactgtata tggcgtataa aggaagcctt caagttgatg
     6841 atggtgtaat ctttgaagca ttgagaaaag cagaagagat aggagggatt attggtttcc
     6901 attgtgaaaa tggggacata atatgtgaac ttgtggataa agcaaagtca gaaaataatt
     6961 tatctccaaa atatcatcaa ttaactagac cagctataat ggaggcagaa gctacatcaa
     7021 gacttatgaa gatagctgaa gttgcaaact ctaaagtata tgtagttcac ttaagctgta
     7081 aagaatcctt agaggaggtt ttaaaagcta agcaaagagg tgtggatgta atagttgaaa
     7141 cttgtcctca ataccttcta ttaaatgatg aattatatga tttagatgga tttgaaagtg
     7201 ctaagtatgt aatgtcacca cctttaagaa ataaagaaaa taatgaaata ttatggaatg
     7261 ctattttaga taatcaaata gatactatag gaacagacca ttgttctttt aattataagg
     7321 gacagaagga gcttgggata aacgatttca gcaagatacc aaatggttct ccaggtgtag
     7381 aacataggat gagtctttta tacacatatg gagtctgtaa aaataaaata tctatcaaca
     7441 agatggtaga agtaacatca actaatgcag caaagttgtt tggaatgtat cctaataaag
     7501 gaactataga aataggcagt gatgcagata tattagtctt aaatccaaac aaaaaatcca
     7561 aaatagaagc gaaaaaacaa ttacaaaatg tagattatac accttatgaa gggtatgaag
     7621 tggattgtca aatagaacat gtttatttaa gaggaaaaga agtagttaga gatggagaat
     7681 taatctgtaa tactccaagt gggagatata taaataggcg tctaacaaag ggggctagag
     7741 aatggtagaa tttatattaa atggagagaa agttgttgca aaagaaggca aaaaactttt
     7801 agacttcctt agagaagata tgaatataac ttcagttaaa aatggatgtt cagaaggtgc
     7861 ttgtggaaca tgtatggtta taatagatgg taaagctact aaagcatgtg tatttaaaac
     7921 tgataaatta gctgggaaaa acataacaac tatagaggga ttatcagaga gagaaagaga
     7981 tgtttttgca tattcttttg ctagtcaagg agctgtacaa tgtggatttt gtattccagg
     8041 tatggtaatt agttcaaaag cacttataga cgttaataat aatcctactg aagatgatat
     8101 aaagaaagct ttaaaaggca atatatgtag atgtacaggg tatgtaaaaa taattaaagc
     8161 tgtcgattta gcagcaaaga tttttagaga aaatatagat gtgccaaaag taaactgtaa
     8221 gggtctagta ggtgaaaatc ttcctagagt tgatgcagtt ggcaaagcac ttggatattc
     8281 tgaatatgta gatgatatga gaattgaggg tatgatttat ggttcagcag ttagagctaa
     8341 gtatcctaga gctttagtta aaaaaataga tatagaagaa gcaaaagctt atccaggagt
     8401 tgttgcagtt ctaacagcta aagatattcc aggaactata aaggtagggc acttaaagaa
     8461 ggattgggat actttaatac cagaaggtga aataactaga tacttaggag atggaatagt
     8521 tttagtagca gcagaaacta gagaagcgtt agaagaggcc aaaaagctag ttaagataga
     8581 ttatgaggaa ttaacaccat taagtactac agcagatgca cttagagaag atgctccaaa
     8641 gatacatgag acagggaata tacttgttaa ggaacattta gtaagaggaa acgctaaaga
     8701 aaagatagaa aattcaaaat atgttgtaac taaaagatat actacacctg ctacagaaca
     8761 cgcattttta gagcctgaat gtgcagtagc tggtccttat gaagatgatg gagtaataat
     8821 ctattcaaca gaccaagggg tgtttgccac tcaacatgaa tgtgcagata tgttaggatt
     8881 accatatgag aaggtaagag ttataaacaa aatggtagga ggagggtttg gtggaaaaga
     8941 agacatgagt gttcaacacc atgcagcctt acttgcatat catacaaaga gatttgtaaa
     9001 agtacagtta acaagaaaag aaagtattat tgttcatcca aaacgacatg ccacagaaat
     9061 ggaatttacg acagcatgtg atgaaaatgg atacttaacg ggtatgaagg caacgataat
     9121 atctgataca ggtgcatatg cctctcttgg aggaccagtg ctacaaagac tgtgtactca
     9181 tgcagcagga ccatacaatt atcaagatat tgatataaca ggaacagcag tatatactaa
     9241 taaccctcct ggaggagcat ttagaggatt tggagttaca cagagttgtt ttgcaactga
     9301 atgtaatcta aatttacttg ctgaaatggt tggaatatct ccttgggaga taagatataa
     9361 aaatgcaata agacctgggc aagttttacc aaatggtcaa tttgcagatg aaggaacagc
     9421 aatggcagag acactagaag ctgtaaaaga agagtatgaa aagcatccac gtgcaggtat
     9481 agcttgtgct atgaaaaata gtggggttgg agttggtata cctgatgttg gaagatgtag
     9541 attggttata aaagatggca aagttcacat aagaacaagt gcttcttgta taggtcaagg
     9601 gcttggtact gttatgactc aagtagtttg tgaaactgct aacctacttc ctgaaaatat
     9661 agttcatgaa gttccagata ctcatttaac accagactct ggtaatacta cggcttcaag
     9721 acagacagta tttacgggag aagctactaa ggttgcatca aataagttaa aagaagcttt
     9781 agactctggc aaaactttac ttgatttaga gggagaagag ttttatggag aatatgaaag
     9841 tattacagat aaaatgggtt gtgacaaaga aaatccagta agtcatgttg cttatggata
     9901 tgcaacacaa gttgtaattc tggatgaaaa tggaaagcta gaaaaagtag tagcagcaca
     9961 tgatgttgga agagctataa atccaatcag tttagaaggt caaatagaag gtggagtagt
    10021 tatgggtctt ggatatgctc taaccgaaga ttacccagta gttaactctg ttcctacagc
    10081 taagtttggt acattaggtt tatttaaatc tacagatata ccagaaatac aagctttaat
    10141 agttgaaaag aacaaagatt tattggctta tggggcaaaa ggtgtaggag aaatttgtac
    10201 agttcctact gcaccagcag tacaaaatgc ctattatgta tttgataaag aatttagaac
    10261 tgagctacca ctacaaaata ctccatatag taaaaaaaag taacaaaaaa aagaaatatt
    10321 aaataaaaaa atagatgaaa ttgtaaataa aaaaaaccct tttgcataag gtatatttta
    10381 aatgatagaa tatacatata caggagggtt tttcttttat aaaataagtt taataaaatt
    10441 gagaggtgag cagaatgaat tttaataata gtctagaatt aactcagtct cagaagctaa
    10501 taatgactac acaacttaag caatcattat caatattaaa tatgagtaag ttagagttag
    10561 aagaagagat aaaaagagaa gcagagaata acccattagt tgaagtcgag aaaagtgggg
    10621 agataaattg ggaagagtat ataaaagaca tggataggtc aagacgttta gatagaacag
    10681 agataagcta taatcctgat aatgaagtta atctagaaaa tttagttaaa tattcgtcaa
    10741 atttatatga agatttaaaa tttcagataa gcctgtataa acttacagac aaagaattgg
    10801 aagtctgtga gtatataatt gatagtttag atgaggatgg atatttaaga actgaggaaa
    10861 aagtaattgt tgatacctta aagatagatg aagaactttt tgaaaaatgt ttaattagtg
    10921 ttcagcagtt ggaacctagt ggagtagggg caagaagtct ttcagaatgt ctgatgatac
    10981 aaatggctag tttaggaata tataatgaaa ttttagagga aatcgttaca aaagacttaa
    11041 atttaattgc aaataataaa tataaagaga taagtaaaaa gtacaatatg tcattacaaa
    11101 aatgtgttga actaataaac ataattaaga cattagaccc caaaccagga agaacttgtt
    11161 cagtagagaa aagtgtatac attcaacctg atgtaacagt agaaaaaatt gatgatgaat
    11221 ttattgtata tttaaatgag aaagattctt atcaaataag aatcaatagt tattataaag
    11281 acatattaaa aaattcacag tctgatgaaa gtgcaaaaga atttataaaa gaaagactaa
    11341 attcagcaac aggacttatg aaaaatatag agagtaggaa aactacagta cttaagattg
    11401 cagaagagat agtaaaagct caagatgaat ttttgagaaa agggacaaaa tatataaaac
    11461 ctttgaaaat gaaagacata gcagaaaaat tagaatttca tgaatctact ataagtagag
    11521 gtgtaaatgg taaatatatg ctaacacctt ttggcgtata tgaatttagg tactttttca
    11581 gtagcgccat tgagactgag aataatgaaa tggcatctag tacaagcata aaaaaaatta
    11641 taaaggaaac aataaaagac gaaaataaaa agaaaccttt aagtgatgac catatatcta
    11701 agttattaaa agaaaaagga atcaatgttg caagaaggac agttgccaaa tatcgtgaag
    11761 aattaggaat cccatcttca agtaagagaa aagaatacta ataagtaaaa gacaaatatc
    11821 agtatggtaa ggggaaaagg ctaattaaga gatgaatgta gattgggcta aaaattttat
    11881 aaagtaaaat aatagaatat ggaaaagtat taaaatgcta taaggtatta aaaaactggg
    11941 gaacattaaa acattaagat agcaaagaac ataaaaaact aaagaagatt aaaacattaa
    12001 agaacattag agctttgagg aatattagaa ctttaaagaa tattaaaatt tataaaatat
    12061 aaaaatacta aaaagaattg aaaaactggg gaacattaaa atattgaaaa atgctaaaaa
    12121 actagaaaac ataattaaat aagtgcttat aaagaatgat taaattaaat aagtgtttgt
    12181 aaagaataat tagatattag aaaaagaaga atacctagat aaataaacta ttattaaatt
    12241 taataatcac aaaaggaaaa cacgtagggt ataaataaaa atcttaacta gataaaagta
    12301 aacttagttg tagataaaaa taactagaaa taaaagtaac tagaaataaa attatttata
    12361 gtaataaata ttagaatgaa aatataaaaa aatatatata aaaagcctta tataaaaaga
    12421 aaaaatactg tgatttgaaa atggtggaga aatattttat aaaataaaaa aataaaaaag
    12481 tttttgaaaa actattgaaa tctatacgca aatcctataa aataaaaata tcttgtggga
    12541 cttaaataaa cactagggac aaaaaaagtc ccgaaaggaa agcaataagg agtatgttat
    12601 gaagaactta ttagaaattc aaaagaaatt gattccacag gcaattgaat ttatggaaag
    12661 aagatactct attttaagac aaatatcttt aagtgaacca ataggtagga gaacattatc
    12721 aaatgtatta gagattagtg aaagaataat caggtcagaa actgagtttt taaaagaaca
    12781 aggacttata gacgtagctg tatctgggat gactatcact aaagaaggtt ctgatttgtt
    12841 agataaactt aaggacatca tgagtgacat catgggattg tctaagttac aagatagagt
    12901 cagagaaaag cttggaataa aaagggtaat attagttcct ggaagctgtg atgaaaatgg
    12961 aagtttatta aaagatgtaa ctagatatgg atgcgaatat ttcttaggca ttttaaaaga
    13021 tggagatatc gtttcaataa caggaggaag cactatgttg gagttttcaa acgttattaa
    13081 aactgataag agatatccaa actctacaat agttccagct agaggaagta tgggaactga
    13141 tgtagagatg caatctaaca atatagttgc ttcaataagt aagaaattac attctaattg
    13201 caaactttta catataccag atgagttgga tgaaagtgca atgaaaactc ttagccaagt
    13261 gccagagatt aagacaactt tggagtatat taaaagaaca gacattttag tttttagtat
    13321 aggtagagca gatgtcatgg caaagagaag aaaacttcca gaagacaagg ttgatgagat
    13381 attatctaaa aatgcagttg gagaagcatt tggatattat ttcaacaaag atggagaaat
    13441 agtttacaaa ctgaacactg ttggaattga tttagaaaca gttaaacaag taaaagaaac
    13501 tattgcaatc tttgcaggga ctgaaaaagt agaagcctta ttagctatat caaatatgca
    13561 taaaaacatg gttcttgtaa cagatgaaga aagtgcttat aagatattgt cattattata
    13621 aaagtcaaga cctagcacag agctagaaaa catacattta ctttatagga ggtagcgaaa
    13681 atggttaaag tagctataaa tggatttgga agaataggaa gattagcatt aagaaaaatg
    13741 atggagcaac aagataagtt cgaagttgtt gcaataaacg acttaacaga cgctaaaatg
    13801 ttagctcatt tatttaaata tgatacagct caaggaagat ttaacggaga aatagaagtt
    13861 aaagaaggtg cttttgtagt aaacggaaaa gaaataaaag taacagcaga aagaaatcct
    13921 gctgacctac catgggctga attaggagta gatatagtat tagaatgtac aggattcttt
    13981 acatctaaag ataaagctga agctcatata caagcagggg ctaagaaagt tgttatatct
    14041 gcaccagcta ctggagattt aaaaacaata gtattcaaca caaacagtga catattagat
    14101 ggttctgaaa cagttatatc aggagcttct tgtactacta actgtttagc tccaatggct
    14161 aaagtattaa atgataaata cggtatagaa aaaggattaa tgactactat acatgcatac
    14221 actaatgacc aaaatacttt agatggtcct catccaaaag gtgatttaag aagagctaga
    14281 gctgctgctg gaaacatagt tccaaacact acaggagctg caaaagcaat aggcttagta
    14341 ataccttcat taaaaggaaa attagatggg gcagcacaaa gagtaccagt tgtaactggt
    14401 tcaataactg aattagtttg tacattagga aaaaatgtta cagttgaaga aataaatgca
    14461 gctatgaaag aagcttcaaa cgaatctttc ggatatactg aagaaatgtt agtatcttct
    14521 gacataatcg gaataagcta cggttcatta tttgacgcta ctcaaacaaa agttatggaa
    14581 gtagatggaa aacaattagt taaagttgtt tcttggtatg ataatgaaat gtcttatact
    14641 tctcaattaa taagaacttt aggatacttt gcacaattag caaaatagtc agttttgaga
    14701 aaatagaaaa gtagattgac aaagtccgag tttgtagttt aatactacgg actcaggact
    14761 ttttttaaga aaaaatatat aaaatacacc taaataatgt tacatactat atataaaaat
    14821 atgataatat gatatattta atagggtaaa agtatgtact atattatact tggcaataaa
    14881 aaattacttt attaaaagga gagatattta tgtcaatgct taacaaaaaa actatagaag
    14941 atattgatgt gtgcggaaaa aaagtattag taagatgtga ctttaatgtg ccattacaag
    15001 atggtgtaat tactgatgaa aatagattga acggagcttt accaactatt caatatttaa
    15061 tttctaaagg ggcaaaagtt atattatgct ctcacttagg aaagccaaaa ggagaagcta
    15121 aaccagaatt atctttagca ccagtagcaa agagattatc tgagatgtta ggaaaagaag
    15181 ttgtatttgc agcagacgat aatgttgtag gagaaaatgc aaagaaagca actgaaaaaa
    15241 tggaaaacgg agatgtagta ttattagaaa atactagata tagaaaagaa gaaactaaaa
    15301 acgaagagaa tttctctaaa gaactagcat cacttgctga aatatttgta aatgatgcat
    15361 ttggaactgc tcatagagca cattgctcaa cagttggtgc aggcgagttt ttacaagaga
    15421 gagtttgtgg atacttaatt caaaaagaat taaaattctt aggagaagct gttgcaaacc
    15481 ctgtaagacc atttacagct atacttggag gagctaaagt ttctgataaa ttagctgtta
    15541 taaacgaatt attagaaaaa gttgataatc taataatagg tggaggaatg gcttatacat
    15601 tcttaaaagc tcagggttat gaagtaggaa cttctttact tgaaatagat aaagttgaat
    15661 atgctaaaga aatgatggaa aaagctaaga ataagggtgt taacttatta ttaccagtag
    15721 acgttgttat ggcagaccac tttgcaccag atgctactcc aatagtaaca gaagatgcta
    15781 atgtaaaaga agattacatg ggactagata tgggacctaa gactatagca aactttgtaa
    15841 aaactataaa agaatcaaaa actgtagtat ggaatgggcc aatgggagta tttgaatttg
    15901 aaaactttgc aaatggtaca ttatcagtag ctagagctat ggcagagttg actgatgcaa
    15961 ctactgtaat tggcggtgga gatagtgcag cagcagttaa tcaattagga tttggtgaca
    16021 agatgactca cgtatcaaca ggtggaggag catcattaga atttttagaa ggtaaagaat
    16081 taccaggtat tgcagcatta gataataaat aaactaatat atagaggaga ttgtaaaatg
    16141 agaaaaccta taattgcagg aaactggaaa atgcataaaa caataaaaga agctttagaa
    16201 tttgtaaatg aagttaaaga taaagtaaat tctgataaag tagaagctgt tatatgtgca
    16261 ccatttactt tattaaagga tttaaaagaa gctactaagg gtacaaatat aaagataggt
    16321 gctcaaaata tgcactttga agaaaaagga gcatttacag gagaagtttc acctcttatg
    16381 ttaaaagaaa tagatatgga ctatgttgta ataggacatt cagaaagaag acaatacttc
    16441 aatgaaactg atgaaactgt aaacaagaaa gttttaaaag cattagaagt aggaatagac
    16501 ccaatattat gtgttggaga aactttagaa caaagagaag ctggaaaaac taaagatgtt
    16561 tgtaaagttc aagttgaaaa agctttagaa aatgttctta aagatgattt agcaaaagta
    16621 gttgttgcat atgagccaat ctgggctatt ggaactggta aaactgcaac tgctgaagat
    16681 gctaatgacg ttatatctta tataagagaa gttataaaag gattatatgg agaattagct
    16741 aatgaagtta gaatacaata cggtggaagt gttaaacctt caaatgtagc cgaaataatg
    16801 ggtcaaagtg atatagatgg agctttagta ggtggagcta gtttagctag taatgattac
    16861 ctagaccttg ttaatttcta aggagtgaaa tgatgaaaaa accagttgct ttaattatta
    16921 tggacgggtt tggatacaat aaggatgtta agggaaatgc aattgctgag tccaaaactc
    16981 ctaatttaga tagaataaaa aaagagtatc caaacacatt gataaatgct agtggtcttg
    17041 atgttggtct acctgatggt caaatgggaa attcagaagt tggacacact aacataggtg
    17101 caggtagaat agtatatcaa gatttaacta gaataacaaa atctataaaa gatggtgact
    17161 tttttactaa taaagtttta tgtgaagcaa tggataatgc aaaagaaaac tctcttcatg
    17221 taatgggact tttgtctgat ggaggagtac attcacatat agaccatctt aaggctataa
    17281 tcaaaatggc aaaagataaa ggcgtacaaa aggtgtatgt tcacgcattt actgatggta
    17341 gagatactga ccctcaaagt gcattagaat atgccaaaga agtacaagtt agtatggatg
    17401 aaattggagt aggtgaattt gctacagttt ctggtagata ctacgctatg gatagagata
    17461 agagatggga aagagtagaa cttgcataca atgccatggt tagaggtatt ggtgaaaaag
    17521 caaattctat tgaggaagct attcaaaact cttatgatga tggaaaaaat gatgagttta
    17581 taatgccaac agttattatg aaagatgata aaccagttgg aagtataaag gaaaatgatt
    17641 caattatatt cttcaacttt agaccagata gagctagaca aataactaga gctttagttt
    17701 gtgaagagtt tgatggattt aaaagagaag atattaaaaa cttctttgta tgcttaacag
    17761 agtacgatat aactatagag aatgtgcata tagcatttgg acctcaatct ttagccaaca
    17821 ctctaggtga gtatctagct aaaaatggaa aaactcaact tagagctgct gaaactgaaa
    17881 agtatgcaca tgtaactttc ttcttcaatg gtggagtaga agagccaaat aaaggtgaag
    17941 aaagactatt gataccttct ccaaaggttg caacttatga tttaaaacca gaaatgtcag
    18001 catatgaatt gacagataaa gctttagata aactaggaga agataagttt gactttatag
    18061 tattaaactt tgcaaatcct gatatggtag gtcatacagg aagtatagaa gcggctataa
    18121 aggctgttga aactgtagat acttgtgtag gaaaattgat agataaaata gtagagttag
    18181 gtggaagtgc aataataact gctgaccacg gaaatgctga atatatgtta gacccagaaa
    18241 caggaaaaac tgtaacggct cactctatta atccagtacc atttattgta gttggtcaag
    18301 aatatgaaag tgcaaaatta ttggatggag gaagattatc tgatatagca ccgactgttt
    18361 tagatatgat gaagttagaa aagccagaag aaatgactgg tcattcactt atttcaaaat
    18421 agtcgatttt gataagtaga gaataatata taaactacaa tataaattta aaaataatgc
    18481 agtttaggtt tattttgtaa agtttagact ttatgacata agatttaaat tttgtaagtt
    18541 ctggatattg taaggtgtag atgaagatat gtttta
//
LOCUS       DBGJFF010000075        18560 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.076, whole genome shotgun sequence.
ACCESSION   DBGJFF010000075 DBGJFF010000000
VERSION     DBGJFF010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18560
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.076"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(365..1432)
                     /gene="rsgA"
                     /locus_tag="ACOIAF_002495"
     CDS             complement(365..1432)
                     /gene="rsgA"
                     /locus_tag="ACOIAF_002495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HGZ2669502.1"
                     /translation="MNGMKQINLQDYGLNRNFITQSMEYHDLYLARIISQEKNFYQVV
                     CVNGVLFAEVSGKLRFEATIQSDFPTVGDFVMLDRCNNKNGNAIIHHVLHRKSLFVRK
                     AAGKVNEEQVVAANIDIVFLCMSLNKDFNIHRLERYLSLTWESGAIPVVVLTKSDLCD
                     NVHRKLQVVNSIALGVDVVVTSSIEPDGYKQISKYIKPGCTVALLGSSGVGKSSLINC
                     LMGGEFLNTKEIRIDDDKGRHATTRRELFLISGGGIVIDTPGMRELGMWDVSEGLDKS
                     FADIEAYFGKCHFRNCTHTSEPGCAIWEAIAHQELSPKRWESYRRLKTEAVFADNKSE
                     YLAKKEKKFKNISKINKKIRR"
     gene            complement(1429..1830)
                     /locus_tag="ACOIAF_002496"
     CDS             complement(1429..1830)
                     /locus_tag="ACOIAF_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNHCP domain-containing protein"
                     /protein_id="HGZ2669503.1"
                     /translation="MNYKNRKRAYYQENSCMDNFICKNCGCLVVPEGAGTQHRNHCPH
                     CLHSLHVDIIPGDRAADCDANMEPIGVWVRKNGEWAIIHRCTRCGHLSSNRIAADDNS
                     VKLISIALKPLAQPPFPLERIIEKLEKEETE"
     regulatory      2626..2722
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            2774..2950
                     /locus_tag="ACOIAF_002497"
     CDS             2774..2950
                     /locus_tag="ACOIAF_002497"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669504.1"
                     /translation="MFRDKMDRCTHMLTAYIGSSYDYCDFIDTQLDDFILEYGENVVE
                     SYLHQVMVLVSKYN"
     gene            3360..3998
                     /locus_tag="ACOIAF_002498"
     CDS             3360..3998
                     /locus_tag="ACOIAF_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669505.1"
                     /translation="MSNQRKDFDITNDMFQESIPITTKIILEDMPSNDELNHVFSKGC
                     ERKMKKRKIVLITLLLIGVLLLGSILYNLFLGKTANISMLKESWNFDIPIPNKEIEVF
                     DTQDSINGDGQSYFIQGFSEKNFKKVFNLKGGIVVSKDNINEIEKYIDKFKRDSVNIN
                     KSNKNKIEEDFKKYKLEVKKDDKYIYKRNYENYVVLIIKKDEQKLYSLIWNQ"
     gene            complement(4487..6247)
                     /locus_tag="ACOIAF_002499"
     CDS             complement(4487..6247)
                     /locus_tag="ACOIAF_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HGZ2669506.1"
                     /translation="MQEVEVIKAKNVLARQARGKAIERLRVAAYCRVSTDSEDQLNSY
                     KSQVQYYTDMIKKNKEWVLADIYADEAITGTQVTKREDFQRMINDCMNGEIDMVFTKS
                     ISRFARNTLDTLKYVRMLKERNIAVYFEDEKINTLTMDGELLLVVLSSVAQQEVENIS
                     ANVKKGLKMKMKRGELVGFQGCIGYDYHPEDKTITVNQEEAEIVRYIFNRYVDGAGGS
                     VIGQELESLGYKTKYGSSTWAPSTVIGIIKNEKYKGDILLGKTFTVDPISKRRLENMG
                     EEDKFYMKDHHEPIISEEVFEKAQEILNRRNKNRTTVGSGKREKYSRKYAFSCMLECG
                     FCGGTLTRRNWHSGSKYSKVIWQCVTATKKGKKFCKHSKGIPETAIEEAFVESYRLLC
                     DDNKDVLEEFLQRMDDTLSSSVVSKQLAKAEKEIDALEKKKSKLVDMRLEEIIDKETY
                     ESKYADLVSKQEQLVEERQKLQETSDNEKDIKKRLKEFKKTLEQNEVLDKFDRYVFES
                     IVEKVIVGGLDENGNVDPAQLTFVYKTGLKNSVDGAKFKTQRKNARGRHRTNELCSHD
                     SNEVDKMCSDSSNDTCGNCS"
     gene            complement(6310..6453)
                     /locus_tag="ACOIAF_002500"
     CDS             complement(6310..6453)
                     /locus_tag="ACOIAF_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HGZ2669507.1"
                     /translation="MNNIIRYSMQIAMLKQLLARKLISEKEYTLVKQRLMKDYKVLSD
                     ITA"
     gene            complement(6772..7011)
                     /locus_tag="ACOIAF_002501"
     CDS             complement(6772..7011)
                     /locus_tag="ACOIAF_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002592280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGZ2669508.1"
                     /translation="MMKSTKKCPSFSTISSAADGNEVAIEKILNHYDAYISKASLRPF
                     YDEHGNMYIVLDMELKGRIRAALIKVILGFEVKVK"
     gene            complement(7016..7264)
                     /locus_tag="ACOIAF_002502"
     CDS             complement(7016..7264)
                     /locus_tag="ACOIAF_002502"
                     /inference="COORDINATES: protein motif:HMM:NF019886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 region 4 domain-containing protein"
                     /protein_id="HGZ2669509.1"
                     /translation="MDEYCVEFTSFDVFGSEVHIYDERLCEAIKELTESRRNILLMSY
                     FLEMTDAEIAEVMEMERFSVCRNRLHTLKLIKDMYEEE"
     gene            complement(8779..8946)
                     /locus_tag="ACOIAF_002503"
     CDS             complement(8779..8946)
                     /locus_tag="ACOIAF_002503"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669510.1"
                     /translation="MNWKELWMTLFGTTEWLGLNIGFWVAMAVVLLIVIVMNVVFWSM
                     KPKKDTKESYK"
     gene            complement(8959..11724)
                     /gene="mgtA"
                     /locus_tag="ACOIAF_002504"
     CDS             complement(8959..11724)
                     /gene="mgtA"
                     /locus_tag="ACOIAF_002504"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002571344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HGZ2669511.1"
                     /translation="MNKKENRMAIRQTVEKAVIRDEQNRRIQFAATNPIKEVLKKLHT
                     TLRGLEVDEVSVSRAKYGTNKVTHEKKKSLAKRLVGAFINPFTAILFCLALVSTITDM
                     IFPYFSLFGSVPEDFDSLTVVIILTMVFISGTLRFVQESRSGNAAEKLLAMITTTCTV
                     TRRNYEKKELPMDDLVVGDIVHLSAGDMIPADVRILDAKDLFVSQASLTGESEPIEKT
                     PNVSAQKESVTDYTNIAFMGSNVISGSATAVVVCVGDHTLFGSMASAVAGEAVETSFT
                     KGVNAVSWVLIRFMLIMVPLVFFVNGITKGDWLAAFLFGVSIAVGLTPEMLPMIVTTC
                     LAKGAVSMSKKQTIVKNLNSIQNFGAIDILCTDKTGTLTQDKVVLEYHLNVNGEDDTR
                     VLRHAYLNSYFQTGYKNLMDLAIIHKTEEEEAADSRLIDLSENYVKVDEIPFDFTRRR
                     LTTVVQDNKGKTQMVTKGAVEEMLSICAFAECNGGVQPLTDDVRYRILETVDNLNDKG
                     FRVLAIAQKSNPSPIGAFGVKDECDMVLIGYLAFLDPPKESTADAIKALKDHGVITKI
                     LTGDNDKVTRTICKQVGLEVRNMLLGSDLEQMGDAELARVAESTDVFAKLTPDQKARV
                     VSVLRENGHTVGFMGDGINDAAAMKAADIGISVDTAVDVAKESADIILLEKDLMVLEQ
                     GIIEGRKTYANMIKYIKMTASSNFGNMFSVLAASALLPFLPMMSVHLIFLNLIYDLSC
                     TAIPWDNVDEEFITKPRKWDASSVGSFMIWIGPTSSIFDFTTYIFMYFVFCPLFVSNG
                     VLFNDLPAHFSGAELAMLQAQYIGMFQAGWFVESMWSQTLVIHMIRTPKLPFVQSRAS
                     APVTLLTMTGIIVLTIIPFTVFGTMLGFVALPAAYFAYLIPCILLYMTLATSLKKAYV
                     RHFGELL"
     gene            complement(11890..12201)
                     /locus_tag="ACOIAF_002505"
     CDS             complement(11890..12201)
                     /locus_tag="ACOIAF_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3784 domain-containing protein"
                     /protein_id="HGZ2669512.1"
                     /translation="MDFTCIFFGILFTLAGFWLAFGKGYRHLSLWKNMPQEEKDKINI
                     VPLCRNVGEMIALNGIIFLMKGFLPGFSNHWFVFAIIAWLIVAGFDVWYIEKSVRYRN
                     Q"
     gene            complement(12448..13125)
                     /locus_tag="ACOIAF_002506"
     CDS             complement(12448..13125)
                     /locus_tag="ACOIAF_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018597221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HGZ2669513.1"
                     /translation="MFYTDFILRIALSLTLGFLIGLERQLTGHPAGIRINVLICMGTS
                     FFTLFPMLYGSDQVFRVGSSIISGVGFLCSGVIFKDRGTVRGMNTAATLWCTAAIGIL
                     ASTGMYAMAITAATILIGSNLILRPFARKFHPITSSDESEKQYRISVTCQEKAEQDIR
                     LLLINSNSCKTLFLNNLESGDVVGDKVEIVAEYCSVGKSKNNILEGIVGQVLVIPEVI
                     SAGWEVL"
     gene            complement(13263..13766)
                     /locus_tag="ACOIAF_002507"
     CDS             complement(13263..13766)
                     /locus_tag="ACOIAF_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005340338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HGZ2669514.1"
                     /translation="MKKNISYIPMDSALEIWLLEKLLRDPPDSDCAKDLLSGIQEMIR
                     GEVSSLLLGASEESDWPVGTLSDRILAGDLEIYPRGRKVLIKGSEVNLTPKEFDILYF
                     LVQNKGEVFTKEQIYRAVWEEDYILDNSNIMAFIRKLRKKIEPAPDCPTYILTIWGIG
                     YKFNDQI"
     gene            complement(13782..16445)
                     /gene="mgtA"
                     /locus_tag="ACOIAF_002508"
     CDS             complement(13782..16445)
                     /gene="mgtA"
                     /locus_tag="ACOIAF_002508"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HGZ2669515.1"
                     /translation="MSKATLFDSRIKKYAYCRTSEIYRDIGSSPDGLSIEQIGSMREK
                     YGANSFNGRKNDTTMQRLRRAFINPFHVILFVLGIVSLVTDVFVASNFARNATTAIII
                     FSMIVISGVIRLIQELRAKSAAAQLDRLVHESVTVRRDGKLIEIPGEELVVGDIVLFS
                     AGDRVPADIRLTKITDLFISQAAITGESAIIEKSCRKLSYGEQDTLTQLENLAFMATT
                     VISGKGEGIVLAVGKDTLYGGFTKPDSDDKNSFKKGADSIAWVMIRFMAILVPIVFIL
                     LGITDGKWMESFAFALSVAVGLMPEMLPMVITACLAKGSLAMSKKQTIIKDINAMQSF
                     GSMDVLCMDKTGTLTNESILLEYYMDIFGNENAEVLDLAYLNSCYHSGVCNTIDNAIL
                     ACNTMPGREAHYANLLTKFQKADEIPFDYARKFVSTLVQDSNGNSQLIMKGDIEHIFS
                     RCNHVEYRGKLLPMGQDARQSVSSVVGEMLQDGMKVIAVARKNVGAQKKITPGDEWGM
                     TLVGYLSFFDAPKQTAKESVTALKRLKVIPKILTGDQAAIAVSVCRRVGIYTERILTG
                     TQLDEMTDCELKKAVEHTHVFAELTPGHKVRLVSAMQDNGHSVGFLGDGVNDIPALNE
                     ANVGISVDTAVDAAKDAADVVLLQKDLNVLEQGVMEGRKTFTNMLKYIKITASSNFGN
                     IFSIICASAFLPFLPMTSIQILLLNLLYDTLCIVLPWDNVDEEEMLSPRDWSGKTLRQ
                     FMLSFGPISSLFDIVTFLFLYYFLCPMLCGGATYLQLTDPSLQLQYVALFQTGWFLES
                     MWTQVLILHFLRTSKIPFFQSRASAPVISITLVGIIAFTSLTFVGGASLFGLTKLPLW
                     YFVFLLLVVSLYMLLNSVAKYFYKKKYQELI"
     gene            complement(16538..16690)
                     /locus_tag="ACOIAF_002509"
     CDS             complement(16538..16690)
                     /locus_tag="ACOIAF_002509"
                     /inference="COORDINATES: protein motif:HMM:NF024055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HGZ2669516.1"
                     /translation="MPIYVRERYEKMLAVSIRAPNLEGMVENFDKDLSRPNETDLIEL
                     KNAISK"
     gene            16706..17032
                     /locus_tag="ACOIAF_002510"
     CDS             16706..17032
                     /locus_tag="ACOIAF_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669517.1"
                     /translation="MSADKLLLGFHLDHVSNLNELLRKVAHNNICIMSDRMTKVAVWK
                     PSVKTIQLVCMEYLNYIIDHSVIYEKIQCANWNGTEETNSIFNNSPSPLITLIYSCDL
                     QQNKTT"
     gene            complement(17231..18133)
                     /locus_tag="ACOIAF_002511"
     CDS             complement(17231..18133)
                     /locus_tag="ACOIAF_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HGZ2669518.1"
                     /translation="MFKKNKKQTENVKEKKVRTVKVGTHKKTVIALWVVLIASVSFGV
                     YKNFTAIDQHTTHEKEVIELRLQDTNGIENFVKNFVKSYYTWSNSKEAIDARTQAISG
                     YLTKELQDLNMDTIRTDIPTSSAITDVIVWSIEQSGTDTFSATYEVDQQIKEGEQTTA
                     VKATYTVKVHVDAGGNMVIIQNPTLAPAIEKSDYEPKTPEADASVDADTINDATAFLE
                     TFFKLYPTATEKELAYYVSGNVLESIGRDYLYSELVNPIFTVDGDNVKVKVAVKFIDN
                     QTKATQVSQFELVLHKESNWKIIG"
     gene            complement(18150..>18560)
                     /locus_tag="ACOIAF_002512"
     CDS             complement(18150..>18560)
                     /locus_tag="ACOIAF_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HGZ2669519.1"
                     /translation="VNQYLTVPQVSGELAQKVMNEALKYQGWKYVFGGSSPTTSFDCS
                     GLTQWCYGKAGISLPRTAQAQYDATQHLPLSQAKAGDLVFFHSTYNAGSYVTHVGIYV
                     GNNQMYHAGDPIGYTDLSSSYWQQHLIGAGRVKQ"
BASE COUNT         5954 a         3812 c         2909 g         5885 t
ORIGIN      
        1 tattatttgt attttataat tcatatattt ttactttata aattagcaat attgcagttt
       61 tgctcttaag aaacatctct atcatacata agcatatact ctattatgta ttagtataaa
      121 tatgcctttt cttatcttac ataagacctc tttataaagg agcctatcgt aacaaatatg
      181 tatacattat cacatgtata tatcatcata taaaaaatca aactttatta aaaaaattaa
      241 taaattgctt taaataatac cacaaaccaa atttgccatc aaaatcacat taaagtaaca
      301 aattgatggc aaatttaata tattaatagt atatagactc atataatgaa caaaaaaact
      361 acacttatct tctaatcttc ttatttattt ttgagatatt tttaaatttc ttttcttttt
      421 ttgctaagta ttcgctttta ttgtctgcaa atactgcttc agttttaagc ctacgatagg
      481 actcccatct tttaggcgaa agttcttgat gagcaatagc ttcccagata gcacagcctg
      541 gttctgaggt gtgagtacaa ttacgaaaat gacattttcc gaaataagct tctatatctg
      601 caaaactttt atctaaacct tctgaaacat cccacattcc caactctctc atacctggtg
      661 tgtcaataac aataccacca cctgaaatta aaaatagctc tcgtctagtc gtggcatgtc
      721 ttcctttatc atcatctatg cgaatttcct ttgtatttaa aaattctcca cccatcagac
      781 aattaattaa tgaagatttt ccaactcctg aagaaccaag taaggcaaca gtacagcctg
      841 gtttaatata cttagaaatc tgtttataac catctggctc tatagatgag gtaacaacta
      901 catctacacc caaggctata gaattaacta cttgtaattt acgatgcaca ttgtcacata
      961 agtctgattt agtaagtact actactggga tagctccact ttcccatgtt aaagacagat
     1021 acctctccaa tctatgaatg ttaaaatcct tattcagtga catgcacaaa aaaacaatgt
     1081 caatattagc tgcaaccacc tgctcctcat ttacctttcc agcagcctta cgcacaaaca
     1141 agctttttct atgaagcaca tgatgaataa tagcattacc atttttatta ttgcaacgat
     1201 ccaacataac gaaatcacca actgtgggaa aatcagattg tatagtagcc tcaaaacgca
     1261 gttttcctga aacttctgca aacagtacac catttacaca tacaacttga taaaagttct
     1321 tttcctgcga gataatccga gcaaggtata aatcatgata ttccatactt tgagtaataa
     1381 aattcctgtt tagaccatag tcttgtaaat tgatttgttt cataccattc attctgtttc
     1441 ctccttttcc aacttttcaa tgattctttc tagtgggaaa gggggctgtg ccagtggttt
     1501 taatgcaatt gatattaatt tcactgaatt atcatcagct gcaatacgat tagaactaag
     1561 atgtccacag cgtgtacaac gatgaataat tgcccactca ccattttttc tcacccacac
     1621 tccaataggt tccatattag catcacaatc tgcagcacgg tcaccaggaa tgatatctac
     1681 atgaagacta tgtaaacaat gtggacaatg atttctatgt tgtgtaccag ctccttcagg
     1741 aactacaagg caaccacaat ttttacaaat aaagttatcc atacaggaat tttcctgata
     1801 ataagctcgt tttctatttt tataattcat ttttatttcc tccatatcgt gtattttcta
     1861 caaaaaaagt ccacaggtat ttatatctgc ctttttatgt ggtatacata tttacctaca
     1921 caaaaacaga aaacccctgt ggactagaaa atttagtatc ttctatttca acataattat
     1981 attcaaaata atactgtgta aaattaataa aagtgtagca gattaaagcc cactacacca
     2041 caatttaaca aagtaatata taatattatt atttgttgtc taatgttagt tatctataaa
     2101 ggcagagtaa gacacaacaa tcaaatgtat caaaaataca cttaaaaatt ctgattaaac
     2161 agaacccata tgcgtctcca actatttatt tgttggttac cattatatat aactctgaca
     2221 tcaggcaaga cactccttca ctaataatta ttattgaaat tttatcataa aatataagta
     2281 tgttcaagta taatttaata tattcatata tttatgtttg tctcattttc ttattctata
     2341 aaatctagtg gtattttaaa atttaatgtt cacctagtat taataataaa aattaactgc
     2401 tattatttgt tttctcattt tatgcctatt gctcaattta tctcacttaa attaataaca
     2461 actgtctttt catacaaaac ctacaatcaa gtttttccat atattctaca tgcatagtct
     2521 atttttttga tataaaataa cgttcatatt tgtaaattta tttaattttc aaataagtat
     2581 attgaataat aagcaattct attgtattat tgtaattgta gaataaaact aaatcggcaa
     2641 aactagagaa atttagtgac gcaaagctat agggactaac gcttataaaa taaactatga
     2701 gttatgtcag ccagttgcca aaaagagata ttctttttgt ttttatgaaa gttttttagg
     2761 gggataataa tatatgttta gagataaaat ggatagatgt acacatatgt tgactgctta
     2821 tattggtagt tcatatgatt attgtgattt tatagataca caactagatg attttatact
     2881 agagtacgga gaaaatgtgg tagaatctta tttgcatcaa gtgatggtat tagtaagtaa
     2941 gtataattaa atagatttgg ttttttagag aacccttttt agttttatct actttaaata
     3001 gccttatgtt agaaagctaa ttaagataga tttctagtat tcataatttt attttttatt
     3061 taaaatacat ataataaaga tgtaaatttg tatcaaataa taaatatgag aacttaaatt
     3121 ctcttatcta tgtgaaaata aacatctctt aaaatgaaat tctgtaaaca taaatgattt
     3181 tttagtttga aggattagat taacaaaatt ataaatttgc aaataagcta tattaaaaaa
     3241 tataataatt ttttatataa cttattcttt tataactttt aattttaaaa gaggagaatg
     3301 tatataagag tattcaatgt actagaaaaa atcaaaagaa atattagaag aaatggatat
     3361 tgagtaatca aagaaaagat tttgatatta caaatgatat gtttcaagaa agcattccta
     3421 tcacaacaaa aattattctt gaagacatgc cttcaaatga tgaattaaat catgtttttt
     3481 caaaaggttg tgaacgaaaa atgaaaaaga gaaaaattgt attaatcaca ctactattaa
     3541 taggtgtttt gttattagga agtatcctat ataatctttt tttaggaaaa acagctaata
     3601 tttcaatgct aaaggaatct tggaattttg atataccaat acctaacaaa gaaattgaag
     3661 tatttgatac tcaagatagt attaatggag atggacaaag ttattttatt caaggatttt
     3721 ctgaaaaaaa tttcaaaaaa gtttttaatt taaaaggtgg tatagttgta agcaaagata
     3781 atattaatga aatagaaaaa tatattgata aatttaaaag agattctgtt aacattaata
     3841 aaagtaataa aaataaaatt gaagaagatt ttaaaaaata taagttagaa gttaaaaaag
     3901 atgataagta tatatataag agaaattatg agaactacgt agttttaatt ataaaaaaag
     3961 atgaacaaaa attatacagt ttaatatgga atcagtaggt acatatgtat tattaagaat
     4021 tataaataat aaaaataaat tttagtcaat atacaaaaac cttttaaatt gtctggaaat
     4081 cacctcttca tttgttataa aaacatacaa caaatgaaga ggtgatttta tggttttaaa
     4141 aaaaataatt ataagtggat atgtgctttc acatgtttat caacatttgt aatgcctata
     4201 tttgctaatg aattataaaa atattaaata atattcaact atagattatg taaattactc
     4261 tatgaaaaaa atgagttgta taaaatctgg ctactgtaga ttgactattt cttttaaaat
     4321 tacaattata ataagtttta tagaattaaa aataaaactt tagagatttt tgataacatt
     4381 gtagactaaa tttgccaccg aaatcgcttt acagtggcaa atcggtggca aatacaatat
     4441 attattagta tataatctat ataaacatta ctattgtttg attatcttag ctacagtttc
     4501 cacatgtgtc gttactacta tcggaacaca ttttatcaac ctcgttgctg tcatgtgaac
     4561 acaattcatt agtcctatgc cgtcctcttg catttttcct ttgtgtttta aatttagcac
     4621 catctacgct attcttcaag cctgttttat aaacaaaggt caactgtgca gggtcaacat
     4681 ttccgttttc atcaagtcca cctacaatta ctttttcaac aatgctttca aacacatagc
     4741 ggtcaaattt atcaagaacc tcattctgtt ccagagtttt cttaaactct ttcaaacgtt
     4801 tttttatgtc tttctcatta tcagaagttt cttgtaactt ttgtctttct tcaacaagct
     4861 gttcttgttt acttactaaa tcagcatatt tactttcgta ggtttcctta tcaataattt
     4921 cttccagacg catatccaca agtttacttt ttttcttctc taatgcgtct atctcctttt
     4981 ccgcttttgc aagttgtttt gacaccacac tactactaag agtatcgtcc attctctgca
     5041 aaaattcttc cagaacatct ttattatcat cacataataa acgataactt tctacaaatg
     5101 cttcttcaat agctgtttct ggaatacctt tgctatgttt acaaaatttc tttcctttct
     5161 tcgtagccgt tacacattgc catataacct tgctgtattt tgagccactg tgccaattac
     5221 gtcttgttaa agtaccacca caaaagccac attctagcat acagctaaat gcgtatttcc
     5281 tgctatactt ctcacgctta ccgctgccta ccgttgttcg gttcttattt ctccgattaa
     5341 gaatttcctg tgctttttca aaaacttctt cactaataat cggctcatgg tgatctttca
     5401 tataaaattt atcttcttca cccatgtttt caaggcgacg tttggaaatc ggatcaacag
     5461 taaaagtttt gcctaagagt atatcaccct tgtatttctc attcttaata attccaataa
     5521 ccgtagatgg tgcccatgtg gaactcccat acttcgtttt ataacctaga ctttcaagct
     5581 cctgtccaat aacagaacca ccagcaccat caacataacg gttgaaaata tatctgacaa
     5641 tctctgcttc ttcttgattt acagttattg tcttatcctc tgggtgataa tcatatccaa
     5701 tacaaccttg aaaacctacc agctcgccgc gcttcatttt cattttcaac ccttttttta
     5761 cattggctga aatgttctcg acttcctgct gtgcaacaga actaagaaca acaagcaata
     5821 actctccatc cattgtaagt gtattgattt tttcatcttc aaaataaacg gcgatatttc
     5881 tctcttttag catacgaaca tatttcaatg tgtctaaagt atttctggca aatcttgata
     5941 tggattttgt aaataccatg tcaatttcac cattcataca gtcatttatc atacgctgaa
     6001 aatcttctcg ttttgttact tgtgtccccg taattgcttc atcagcataa atgtcagcta
     6061 acacccattc cttgtttttc ttaatcatgt cagtataata ctgaacttgt gatttataac
     6121 tgtttaactg atcttcgcta tctgtactta cacggcaata tgcagctaca cgcaaacgct
     6181 caatggcttt tccacgggct tgtcttgcca aaacattttt tgcttttatt acttcaactt
     6241 cctgcatagt acacctcctt attttgtgtt cctttcatat tgttattgta ctactttaaa
     6301 aaacacaatt caagcagtta tgtcagaaag caccttatag tctttcatca gcctttgttt
     6361 tacaagagtg tattcttttt cagaaatcag tttacgggca agcagttgtt ttaacatagc
     6421 aatttgcata ctatacctaa ttatattatt cattaaaacc ctccttgctt ttttatttat
     6481 ggaagtaatc agacggcttc ggcagctcca ctggactttc accattccca ttctgatggg
     6541 tgaaccgtgt catacgggtg tatcattatt cagatatacg agtcatggca gcaacctttc
     6601 caaagatcgc ttgcggttca ctggcataat cgctcgctgc tttccccatt tcagtggtcg
     6661 tggcgttcca gtccgctggc tcgtcgtata tcaaacgtat ctgattactg tattcacttg
     6721 tcaaagaacg gaaaagagtt aaaatgaggt ggtagatact ctctgttata tttacttcac
     6781 tttgacttca aaccctaaga ttactttaat caacgctgcc cgtatcctgc ctttcagttc
     6841 catatcaagc acgatataca tattcccgtg ttcgtcatag aacgggcgta ggctggcttt
     6901 tgatatgtaa gcgtcataat ggttcaaaat cttttcaatt gccacctcgt taccgtcagc
     6961 tgcagaactg atggtcgaga atgatggaca cttttttgta gacttcatca tgtttttatt
     7021 cctcctcgta catatcctta ataagcttta atgtgtgcag cctgtttcta catacggaaa
     7081 atcgttccat ttccataacc tctgcaattt cagcgtcggt catttcaaga aagtaggaca
     7141 taagcagaat gttacgtcgg ctttctgtta attctttgat agcttcacat aagcgttcat
     7201 catagatatg gacttctgaa ccaaatacat caaatgacgt aaactcaacg caatattcgt
     7261 ccatggcacc gatctggttt aactccattt cagataaatc acagaacggc gtttcacgtc
     7321 ttgcacgtct gccaagctct cggtcatagt tctttacagt tcgtcctaca accttgcgag
     7381 ctagacaatc aaattgaagt ctgatagcgc cctcgaaaga agatggtttc ataatctcac
     7441 cccctttcca gcgaaagttg ctaaagcgaa aagacttcta cctcttttcg cactaacact
     7501 caccatgaaa ggtgggattt gatacccaac cgcaaaaatt ttttgaaaaa gttttatgca
     7561 gtaaaaaagc acaagggcaa tacagacgta ctgatcttgt gctaaacaaa ttattcctgt
     7621 tatatgctgt aaaaaaccac atataggagc attatgttcc gtagaattta aatgtttttt
     7681 taatgaacta ccgatggtcg aaagttgttg aatatgtaag tgtttcatac ggctatccct
     7741 agagccgata aaacaagacc atccagtaat ttgtccaacg ttaggcaaaa ataaaacggc
     7801 ggctatataa ccgtcgtctt attcaaaagt tcaaaaagaa ctaaactaaa ccctctaaag
     7861 cagtatgtat ttataatcag tcttgcaaaa taacaaagta gaattgaaat ataaggtagc
     7921 aatccttgtg catctaataa acttataggg gttacagcag accacggaat aatattttgt
     7981 atactttcag atcaatatag tccaacaaaa ccaattcccg atatttagta actaaaaagt
     8041 aaactagagt agaaaacatt tctttgcaaa acacaaataa taagtaatgg actcattgac
     8101 aatagattga gaaatccagt tttttagact tagatagatt atctcacttg taaatgattt
     8161 aagttctttg acttcatata tagcggtata atctgtcaac gtgatcatta caaaccataa
     8221 tgcctatata acatactttc caagcaaaaa ctttattcta ttagttgggg aatacaaaat
     8281 atttatcatt gtgcgttcaa catattcttt ttgtataaaa atgtaattac aaaacaccac
     8341 tcaatataag atagataata atttgaatag gaattatcaa tctacgtaac cattcattac
     8401 ctgtcattgt tgttacaata gatttgggat tctgtttcca ttacataatt tttttatcct
     8461 tctttcagat atctcctcat tttatcgaag cttcttctac agacatacat tccataataa
     8521 aatctacctt tgaatattat ttcacgattt cgttatagac gcgtaaacaa acaataaaaa
     8581 acgaaatttc ttttaagtac cataattttt atatcgaaat tacaattatc cacttatata
     8641 cagcatttat aggtaattca atgataaaaa aacacatact ttactactaa attgccacaa
     8701 ttgaatgcgc cttgatatga tagggagatt ccacaataaa aaactgtgca tctcccttga
     8761 ttcattcata ctgatatctt atttataaga ctcttttgtg tctttttttg gtttcatgga
     8821 ccaaaatact acattcatta caataacaat cagcaacaca actgccatag ccacccaaaa
     8881 gccgatatta agaccaagcc attcggtagt tccaaaaagg gtcatccaaa gttctttcca
     8941 gttcatcatt ccacctcctt aaagcagttc tccgaaatga cggacatagg ctttttttaa
     9001 acttgttgcc aaagtcatat acagcagaat acatggaatc agataagcaa agtatgctgc
     9061 aggcaatgca acaaatccaa gcatagttcc aaacacagta aacggaataa tggtcagaac
     9121 tataattcca gtcattgtta gcaaagtcac cggagctgat gcacgactct gaacaaaagg
     9181 tagctttgga gtacgaatca tgtgaatgac taatgtctgg ctccacatag attccacgaa
     9241 ccaccctgct tggaacatcc cgatatactg tgcctgaagc attgccagtt ctgcaccgct
     9301 gaaatgagca ggcaaatcat taaataatac accattcgat acgaacaatg gacaaaatac
     9361 aaagtacata aaaatatagg ttgtaaaatc aaagatagaa ctggttggtc caatccagat
     9421 cataaaactt ccaacactgg aagcgtccca tttacgaggt ttggtaataa actcctcatc
     9481 cacattatcc caaggaattg cagtacagga taaatcataa attaagttta gaaatataag
     9541 atgaacactc atcattggta agaaaggtag caaagcagat gcagcaagca ctgagaacat
     9601 attgccgaag ttggatgaag cagtcatttt aatgtacttg atcatgttgg catatgtctt
     9661 tcgtccctca ataataccct gttccaaaac catcagatct ttttccaata gaataatgtc
     9721 agcagattct tttgccacgt ccacggcggt atccaccgaa ataccaatat cggcggcttt
     9781 catagctgca gcatcattaa taccgtcacc catgaaaccg accgtatgac cattttcacg
     9841 gagaacagaa accacacggg ctttctgatc aggggttagc tttgcaaata catctgtaga
     9901 ctctgccact cttgcaagct ctgcatcgcc catttgttcc aaatcagaac cgagaagcat
     9961 attgcgaacc tctaatccca cctgcttgca gatagttcga gtcactttgt cgttgtcacc
    10021 tgtcaaaatt ttggttataa caccatggtc tttcagagct ttaatagcat ccgcagtaga
    10081 ttcttttgga ggatcaagga atgccagata tccaatcagt accatatcac actcatcttt
    10141 aacaccaaat gcgccgattg gggaaggatt actcttctgt gcaatagcta gtacgcggaa
    10201 tcccttatcg ttaagattgt ccactgtctc cagaattctg taacgaacgt catcagtcag
    10261 aggctgcact cctccattac actctgcaaa cgcacaaata gagagcattt cttccacagc
    10321 accttttgtt accatctggg tcttaccctt attatcctgt accactgtag tcagacgacg
    10381 acgtgtgaag tcaaaaggaa tctcatctac tttcacatag ttttcagaca ggtcaatgag
    10441 acgcgaatct gcggcttcct cttcctccgt cttatggata attgccaaat ccatcaggtt
    10501 tttatagcca gtctggaagt aactattgag gtaagcgtga cgcagtactc gcgtatcgtc
    10561 ctcgccattt acattcagat ggtattccag cacaacctta tcctgggtca gcgtgccagt
    10621 cttgtcagta cagagaatat caatagcacc gaagttctga atggaattga ggtttttaac
    10681 gattgtctgc ttcttgctca tggaaacagc gcctttcgca agacaggttg ttacaatcat
    10741 cggcaacatt tctggtgtca aaccaacagc aatagaaacg ccgaataaga aggccgccaa
    10801 ccaatcgccc ttggtgatac cattgacaaa gaacaccagc ggaaccataa tgagcataaa
    10861 acgaatcaat acccaagaaa cggcattgac acccttggta aagcttgttt caaccgcttc
    10921 tccagcaact gcggacgcca tagagccaaa cagagtatga tcgccaacac aaacaacaac
    10981 agctgttgcg ctaccagaaa ttacattact ccccataaaa gcaatgtttg tgtaatcagt
    11041 cacgctttct ttctgagcac ttacattggg tgttttttca ataggttcac tctcaccagt
    11101 aagacttgcc tgactgacaa acaaatcctt cgcatccaaa atacgaacat ccgccggaat
    11161 catatctcca gcagaaagat gcacaatatc tcccaccacc aaatcatcca taggaagttc
    11221 ttttttttca tagtttctac gagtaactgt acaagtggtc gtaatcatgg ctaacaattt
    11281 ctctgctgca tttccgcttc tggattcctg tacaaaacga agcgtaccgg aaatgaatac
    11341 cattgttaaa atgattacaa cagtcaggga atcaaagtcc tctggcacac tgccaaacag
    11401 agaaaaatac ggaaaaatca tatctgtaat tgtggatact aaagccagac aaaataaaat
    11461 ggcggtaaat ggattgataa atgcgccaac cagtcgtttt gccagagatt tctttttttc
    11521 atgagtgact ttattggtac catatttagc acgacttacg gatacttcat ctacttctaa
    11581 gccacgaagt gtggtatgaa gtttttttaa aacttcttta ataggattgg ttgccgcaaa
    11641 ttgaatgcgg cggttctgtt cgtcacggat gactgctttt tctacagtct gacgaatagc
    11701 catacgattt tcttttttgt tcattggtct tacctccttg aattttcata ggggcaagga
    11761 atattatatg agcgacacgg aatctccaaa ttctcatata atattctctg ccttcgtgga
    11821 ctgacatcag agtccatgtc tttcacctca caatcttaaa aaaatatcta tgtgaaacct
    11881 caatgtggat tattggttgc gatagcggac acttttttcg atataccaga catcaaagcc
    11941 ggcaacaatc agccatgcga ttattgcaaa tacaaaccag tggtttgaaa atcccggcaa
    12001 aaatcctttc attaagaata taatcccatt taacgcaatc atttctccaa cattacgaca
    12061 aagcgggaca atattaattt tatctttctc ctcctgcggc atatttttcc acagggaaag
    12121 atgacgatat ccttttccaa aggccaacca aaagcctgca agagtaaata aaattccaaa
    12181 aaagatacat gtaaaatcca tcgtttgttc ctcctttcaa tgcttaaacc tgtttcttag
    12241 tttcgtcagc ttcagaagtt taatgaagag cacaataata gtaacagcaa gaataaacca
    12301 gatcacaata ctcacctgat accatatctt aaagattacc attctttgtt caaagaaatt
    12361 ccaacccaaa tagctacata agattgtaaa tacagggtag aaagcaaaaa gaattgctcg
    12421 tttcaaatct tcgatttctc tcctcactca caacacctcc caaccagcac taatcacttc
    12481 cggaattacc aatacctgcc caacgattcc ttctaaaata ttgtttttgg attttccgac
    12541 agaacagtat tctgcgacaa tctcaacctt gtcaccaacc acatcaccgc tttccagatt
    12601 gttcaaaaag agcgtcttac aggaattgct gttaatcagc aataagcgta tatcctgctc
    12661 tgccttttcc tgacatgtaa ccgaaatacg atactgcttt tcagattcat cactggaagt
    12721 aattgggtga aactttcttg caaagggacg tagaatcagg ttggaaccaa ttaaaatagt
    12781 agcggcggtg atcgccattg catacattcc cgtacttgca agaattccaa tcgctgcagt
    12841 acaccataag gtggcagcgg tattcattcc acgtactgtc cctctgtcct tgaaaataac
    12901 accactgcat aaaaaaccaa caccggatat aatactgctt ccgacacgga atacctgatc
    12961 cgaaccgtag agcataggaa acaaggtaaa gaaacttgta cccatgcaga ttaacacatt
    13021 gatacgaata cctgcaggat gaccagtcaa ttgccgttct aatccaataa gaaatccaag
    13081 tgttaaggag agggcaattc tcaaaataaa atctgtgtaa aacatatgaa acacctccct
    13141 ttaactacta acagtatata aaaaatccaa atacaatcct atggctaaag tcttgcgatt
    13201 ttcttgcgat ttggtaagaa aatagacgga ctgaaaccga aaatggtctt agtccgtctt
    13261 tgtcatatct gatcattaaa tttgtatcca ataccccaga tggtcaaaat atatgtggga
    13321 caatccggag caggttcaat cttttttcgc aacttccgga taaaagccat aatgttgctg
    13381 ttatccagaa tataatcttc ctcccatacg gcacgataaa tctgttcctt tgtaaaaact
    13441 tcacctttgt tctggacaag aaagtacaaa atatcaaatt cctttggagt caggttgact
    13501 tcagaacctt ttatcagtac ttttctgcct cttggataaa tttccagatc ccctgccagg
    13561 attctatctg acagagtacc cactggccaa tcgctttctt ctgatgctcc taacaataac
    13621 gaagatacct ctccacgaat catctcctga ataccagaaa gtaaatcctt cgcacaatca
    13681 ctgtccggtg gatctcttag tagtttttct aatagccaga tttcaagagc cgaatccatc
    13741 ggaatataac taatattctt tttcatcatg aatgcctcct cttaaatcaa ctcttgatat
    13801 tttttcttat aaaaatattt ggctactgaa ttcagcagca tatagagaga taccaccagc
    13861 agtaaaaata cgaaatacca aagaggtaat ttcgtcagtc caaacaacga cgcaccgccg
    13921 acaaatgtaa gggaagtaaa agcaataatc cccactagag ttatagaaat gaccggtgca
    13981 gatgccctgc tctggaaaaa aggaatcttg gaagttcgca gaaaatgtaa aatcaaaacc
    14041 tgtgtccaca tagattccaa aaaccatcca gtctgaaaca gtgctacata ctgaagttgc
    14101 agggaaggat ctgtaagctg cagataggtt gcccctccac atagcatcgg acataggaaa
    14161 taatacaaaa ataggaaggt gacaatatca aacagagaac tgatcggtcc gaaagacagc
    14221 ataaactgtc tcagtgtttt gccggaccag tctcttgggg acagcatctc ttcctcatct
    14281 acattgtccc aaggaagcac aatacacaac gtatcataca gcagattcaa aagcagaatc
    14341 tgaattgaag tcataggcag gaacggtaaa aaggcactgg cacaaatgat ggaaaaaata
    14401 tttccaaagt tagagctagc ggtgatttta atatatttca gcatattggt aaaagttttt
    14461 cgcccctcca ttacaccttg ctccaaaaca ttcaagtcct tttgcagcaa aaccacatcg
    14521 gcagcatctt ttgctgcgtc cactgccgta tctacagaga ttcccacatt tgcttcattc
    14581 agtgccggaa tgtcgttgac tccatcacca agaaatccca cggaatgacc attatcctgc
    14641 atagcagaga caagacgcac cttatgtccc ggagtaagct ctgcaaaaac atgagtatgt
    14701 tccacagcct ttttaagttc acagtctgtc atttcatcca gttgagtacc agttaagata
    14761 cgctctgtat aaatacccac ccggcggcaa acagacacag caatcgctgc ctggtcccct
    14821 gtcagaatct tagggataac ttttaatctt ttgagtgcag ttacagactc ctttgctgtc
    14881 tgctttggtg catcaaagaa ggatagatag ccaactagag tcatgcccca ttcgtcacca
    14941 ggagttattt tcttttgcgc tcccacattt tttcgtgcca ccgcaatgac cttcattccg
    15001 tcctgtaaca tttcacctac tacagaagat acactctgtc tcgcatcctg ccccatgggg
    15061 agaagttttc cacgatattc tacatggttg cagcgtgaga atatatgttc aatatcacct
    15121 ttcataataa gctgactatt cccattgcta tcctgtacaa gtgtactgac aaacttacga
    15181 gcatagtcaa agggaatttc atcagctttt tggaattttg tcagaagatt tgcgtaatgg
    15241 gcttcccgcc ctggcatggt attgcaggca agaatggcat tatcaatggt attgcagaca
    15301 ccagaatgat agcagctatt cagataagca agatcaagaa cctctgcatt ttcattccca
    15361 aaaatatcca tataatattc cagcaggatg ctttcattgg taagtgttcc tgttttatcc
    15421 atacaaagca catccatgct accaaagctc tgcatggcat taatatcctt gataatggtc
    15481 tgctttttgc tcatagcaag acttcctttg gcaaggcagg ctgtaataac cataggaagc
    15541 atttccggca tcagacctac agcaactgac agggcgaaag caaaagattc catccatttt
    15601 ccatcagtaa tccccagtaa tataaataca atgggaacta atatcgccat aaaacgtatc
    15661 atcacccagg cgatggaatc ggctcctttt ttaaaggaat ttttatcatc agaatccggt
    15721 ttggtaaatc ctccatacag ggtatctttg cctactgcca gtacaatgcc ttcccctttt
    15781 ccactgatga ctgtggttgc cataaaagcg aggttttcaa gctgagtcag tgtatcctgc
    15841 tctccgtaac tgagttttcg gcagctcttt tcaatgatgg cgctctcgcc agtaatcgca
    15901 gcctgagaga taaagagatc cgtaatcttc gtcaatctga tatccgccgg aacacgatca
    15961 cctgcagaga atagtacaat atcccctacc actaattctt cgccaggaat ctctattaac
    16021 tttccatcac gcctcactgt gacgctttca tgaactaggc gatcaagctg agcagcagcg
    16081 ctctttgctc gcagctcctg aatcagacga attacgccac taattacgat catggaaaag
    16141 atgataatgg cggtagtagc atttctcgca aaattggatg ctacgaaaac atccgtaaca
    16201 agagaaacaa tgcccagaac aaaaagaatc acatgaaacg ggttaataaa tgcccgtctc
    16261 aaccgttgca tcgtcgtatc atttttccgc ccattgaagc tgttcgctcc gtatttctcc
    16321 cgcattgaac cgatttgctc aatggataaa ccatccggag atgagccaat gtctcgataa
    16381 atttctgatg ttctacagta tgcgtatttt ttgatacgac tatcaaatag tgttgctttt
    16441 gacacgaaac atcccctcct ttataagtat aagtaataac attttaacac agtgttctga
    16501 aaatgctata tttatattct tgcttttttc ttgcaattta ctttgatatg gcatttttca
    16561 gctcgatcaa atccgtctcg ttagggcgag ataaatcctt atcaaagttt tcaaccattc
    16621 cttctaaatt tggagcacgg atggatacgg caagcatttt ctcatagcgc tctcgcacat
    16681 aaataggcat ttccccttga cacatatgag tgctgacaaa cttcttcttg gttttcacct
    16741 tgaccatgta tcaaatttaa atgaactact tagaaaagtt gctcataata atatttgtat
    16801 tatgagtgat agaatgacaa aagtggcagt atggaaaccg agtgtaaaaa caatacaatt
    16861 agtttgtatg gaatatctga actacataat tgaccactct gtaatttatg aaaaaataca
    16921 atgcgcaaat tggaacggga cagaagaaac caatagtatt tttaataatt ccccttcacc
    16981 attgattact cttatctatt cttgcgattt acaacaaaat aaaacaacat aaattttaaa
    17041 tacgttgact ggcataattc atatgtatag taagtctaca attaagatgc gctttttcta
    17101 atccaaatat attaagaaaa aaattttgcg aagccattaa tactttgttt aagatgtctt
    17161 tcataaatat aaagaataat gctcttaaca aaagcgtgca aattcctaaa gaaatgcacg
    17221 ctttcaataa ttatcctata atcttccaat tgctctcctt atgtagcact aattcaaact
    17281 gcgatacctg tgttgccttt gtctgattat caatgaattt cacggcaacc ttgactttca
    17341 cattgtcccc atcaaccgta aagatagggt ttaccaattc agaatagagg tagtccctgc
    17401 cgatagactc aagcacattt ccagacacat agtaagcaag ctccttttct gtggctgttg
    17461 ggtatagctt aaagaaagtt tccagaaatg cagtagcgtc atttatggta tccgcgtcta
    17521 cacttgcgtc tgcttctggt gtctttggtt catagtctga tttctcgatt gctggtgcaa
    17581 gggtagggtt ctgaataatt accatattcc cgccagcgtc cacatgaact tttacggtat
    17641 aggttgcctt aaccgctgtt gtctgttccc cctcttttat ctgctgatct acttcgtagg
    17701 tagcggaaaa agtgtccgtt ccagactgtt caatgctcca tacaatcaca tctgtaatgg
    17761 cagagctggt cggtatatcc gttctaatgg tatccatatt caagtcctgc aattcctttg
    17821 tcagataacc gctgattgcc tgcgtccttg cgtcaatcgc ttctttgctg ttactccatg
    17881 tgtaatagga ctttacaaaa ttcttcacga aattttctat cccgttggtg tcctgcaagc
    17941 gaagctcaat cacttctttt tcatgggtcg tgtgctggtc aatagcggta aaattcttat
    18001 atactccaaa gctcacgctt gcaataagca ccacccacaa tgcaatcacg gttttcttat
    18061 gtgtgcctac cttgacagta cgtacctttt tttctttgac attttctgtc tgtttcttat
    18121 tcttcttaaa catatttttc aagtcctttc tattgtttta ctcgtcctgc accgattaag
    18181 tgctgttgcc agtaactact acttaggtct gtatatccta tcgggtcgcc tgcatggtac
    18241 atctgattat ttcccacata gataccgacg tgggttacat acgaaccagc gttataggtg
    18301 gaatggaaaa ataccaaatc gccagccttt gcctgcgaga gtggaagatg ttgggtagcg
    18361 tcatactggg cttgtgccgt tctcggtaag gagataccag cttttccata gcaccattgc
    18421 gtcagtcccg aacagtcaaa ggaagtggtt ggagaagaac caccaaacac atatttccac
    18481 ccttgatatt tcaacgcttc attcattacc ttttgtgcca gctctcccga tacctgcgga
    18541 acagttaaat actgattgac
//
LOCUS       DBGJFF010000076        17648 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.077, whole genome shotgun sequence.
ACCESSION   DBGJFF010000076 DBGJFF010000000
VERSION     DBGJFF010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17648
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.077"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            54..608
                     /gene="tcdR"
                     /locus_tag="ACOIAF_002513"
     CDS             54..608
                     /gene="tcdR"
                     /locus_tag="ACOIAF_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin sigma factor TcdR"
                     /protein_id="HGZ2669520.1"
                     /translation="MQKSFYELIVLARNNSVDDLQEILFMFKPLVKKLSRVLHYEEGE
                     TDLIIFFIELIKNIKLSSFSEKSDAIIVKYIHKSLLNKTFELSRRYSKMKFNFVEFDE
                     NILNMKNNYQSKSVFEEDICFFEYILKELSGIQRKVIFYKYLKGYSDREISVKLKISR
                     QAVNKAKNRAFKKIKKDYENYFNL"
     gene            926..8026
                     /gene="tcdB"
                     /locus_tag="ACOIAF_002514"
                     /gene_synonym="toxB"
     CDS             926..8026
                     /gene="tcdB"
                     /locus_tag="ACOIAF_002514"
                     /gene_synonym="toxB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_074049122.1"
                     /note="Toxigenic strains produce this toxin (TcdB) or
                     TcdA. TcdB-producing strains are tumorigenic in mice;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdB"
                     /protein_id="HGZ2669521.1"
                     /translation="MSLVNRKQLEKMANVRFRTQEDEYVAILDALEEYHNMSENTVVE
                     KYLKLKDINSLTDIYIDTYKKSGRNKALKKFKEYLVTEVLELKNNNLTPVEKNLHFVW
                     IGGQINDTAINYINQWKDVNSDYNVNVFYDSNAFLINTLKKTVVESAINDTLESFREN
                     LNDPRFDYNKFFRKRMEIIYDKQKNFINYYKAQREENPELIIDDIVKTYLSNEYSKEI
                     DELNTYIEESLNKITQNSGNDVRNFEEFKNGESFNLYEQELVERWNLAAASDILRISA
                     LKEIGGMYLDVDMLPGIQPDLFESIEKPSSVTVDFWEMTKLEAIMKYKEYIPEYTSEH
                     FDMLDEEVQSSFESVLASKSDKSEIFSSLGDMEASPLEVKIAFNSKGIINQGLISVKD
                     SYCSNLIVKQIENRYKILNNSLNPAISEDNDFNTTTNTFIDSIMAEANADNGRFMMEL
                     GKYLRVGFFPDVKTTINLSGPEAYAAAYQDLLMFKEGSMNIHLIEADLRNFEISKTNI
                     SQSTEQEMASLWSFDDARAKAQFEEYKRNYFEGSLGEDDNLDFSQNIVVDKEYLLEKI
                     SSLARSSERGYIHYIVQLQGDKISYEAACNLFAKTPYDSVLFQKNIEDSEIAYYYNPG
                     DGEIQEIDKYKIPSIISDRPKIKLTFIGHGKDEFNTDIFAGFDVDSLSTEIEAAIDLA
                     KEDISPKSIEINLLGCNMFSYSINVEETYPGKLLLKVKDKISELMPSISQDSIIVSAN
                     QYEVRINSEGRRELLDHSGEWINKEESIIKDISSKEYISFNPKENKITVKSKNLPELS
                     TLLQEIRNNSNSSDIELEEKVMLTECEINVISNIDTQIVEERIEEAKNLTSDSINYIK
                     DEFKLIESISDALCDLKQQNELEDSHFISFEDISETDEGFSIRFINKETGESIFVETE
                     KTIFSEYANHITEEISKIKGTIFDTVNGKLVKKVNLDTTHEVNTLNAAFFIQSLIEYN
                     SSKESLSNLSVAMKVQVYAQLFSTGLNTITDAAKVVELVSTALDETIDLLPTLSEGLP
                     IIATIIDGVSLGAAIKELSETSDPLLRQEIEAKIGIMAVNLTTATTAIITSSLGVASG
                     FSILLVPLAGISAGIPSLVNNELVLRDKATKVVDYFKHVSLVETEGVFTLLDDKIMMP
                     QDDLVISEIDFNNNSIVLGKCEIWRMEGGSGHTVTDDIDHFFSAPSITYREPHLSIYD
                     VLEVQKEELDLSKDLMVLPNAPNRVFAWETGWTPGLRSLENDGTKLLDRIRDNYEGEF
                     YWRYFAFIADALITTLKPRYEDTNIRINLDSNTRSFIVPIITTEYIREKLSYSFYGSG
                     GTYALSLSQYNMGINIELSESDVWIIDVDNVVRDVTIESDKIKKGDLIEGILSTLSIE
                     ENKIILNSHEINFSGEVNGSNGFVSLTFSILEGINAIIEVDLLSKSYKLLISGELKIL
                     MLNSNHIQQKIDYIGFNSELQKNIPYSFVDSEGKENGFINGSTKEGLFVSELPDVVLI
                     SKVYMDDSKPSFGYYSNNLKDVKVITKDNVNILTGYYLKDDIKISLSLTLQDEKTIKL
                     NSVHLDESGVAEILKFMNRKGNTNTSDSLMSFLESMNIKSIFVNFLQSNIKFILDANF
                     IISGTTSIGQFEFICDENDNIQPYFIKFNTLETNYTLYVGNRQNMIVEPNYDLDDSGD
                     ISSTVINFSQKYLYGIDSCVNKVVISPNIYTDEINITPVYETNNTYPEVIVLDANYIN
                     EKINVNINDLSIRYVWSNDGNDFILMSTSEENKVSQVKIRFVNVFKDKTLANKLSFNF
                     SDKQDVPVSEIILSFTPSYYEDGLIGYDLGLVSLYNEKFYINNFGMMVSGLIYINDSL
                     YYFKPPVNNLITGFVTVGDDKYYFNPINGGAASIGETIIDDKNYYFNQSGVLQTGVFS
                     TEDGFKYFAPANTLDENLEGEAIDFTGKLIIDENIYYFDDNYRGAVEWKELDGEMHYF
                     SPETGKAFKGLNQIGDYKYYFNSDGVMQKGFVSINDNKHYFDDSGVMKVGYTEIDGKH
                     FYFAENGEMQIGVFNTEDGFKYFAHHNEDLGNEEGEEISYSGILNFNNKIYYFDDSFT
                     AVVGWKDLEDGSKYYFDEDTAEAYIGLSLINDGQYYFNDDGIMQVGFVTINDKVFYFS
                     DSGIIESGVQNIDDNYFYIDDNGIVQIGVFDTSDGYKYFAPANTVNDNIYGQAVEYSG
                     LVRVGEDVYYFGETYTIETGWIYDMENESDKYYFNPETKKACKGINLIDDIKYYFDEK
                     GIMRTGLISFENNNYYFNENGEMQFGYINIEDKMFYFGEDGVMQIGVFNTPDGFKYFA
                     HQNTLDENFEGESINYTGWLDLDEKRYYFTDEYIAATGSVIIDGEEYYFDPDTAQLVI
                     SE"
     gene            8148..8648
                     /gene="tcdE"
                     /locus_tag="ACOIAF_002515"
     CDS             8148..8648
                     /gene="tcdE"
                     /locus_tag="ACOIAF_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin-like glycosylating toxin export protein
                     TcdE"
                     /protein_id="HGZ2669522.1"
                     /translation="MHSSSPFYISNGNKIFFYINLGGVMNMTISFLSEHIFIKLVILT
                     ISFDTLLGCLSAIKSRKFNSSFGIDGGIRKVAMIACIFFLSVVDILTKFNFLFMLPQD
                     CINFLRLKHLGISEFFSILFILYESVSILKNMCLCGLPVPKRLKEKIAILLDAMTDEM
                     NAKDEK"
     gene            8757..8888
                     /locus_tag="ACOIAF_002516"
     CDS             8757..8888
                     /locus_tag="ACOIAF_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669523.1"
                     /translation="MPRDTQVLNTYNFEASVHYYIDDKVVYQTLVHKDGAWSVGKIY"
     gene            8974..9159
                     /locus_tag="ACOIAF_002517"
                     /pseudo
     CDS             8974..9159
                     /locus_tag="ACOIAF_002517"
                     /inference="COORDINATES: protein motif:HMM:NF022413.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9376..17508
                     /gene="tcdA"
                     /locus_tag="ACOIAF_002518"
                     /gene_synonym="toxA"
     CDS             9376..17508
                     /gene="tcdA"
                     /locus_tag="ACOIAF_002518"
                     /gene_synonym="toxA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_105161403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdA"
                     /protein_id="HGZ2669524.1"
                     /translation="MSLISKEELIKLAYSIRPRENEYKTILTNLDEYNKLTTNNNENK
                     YLQLKKLNESIDVFMNKYKTSSRNRALSNLKKDILKEVILIKNSNTSPVEKNLHFVWI
                     GGEVSDIALEYIKQWADINAEYNIKLWYDSEAFLVNTLKKAIVESSTTEALQLLEEEI
                     QNPQFDNMKFYKKRMEFIYDRQKRFINYYKSQINKPTVPTIDDIIKSHLVSEYNRDET
                     VLESYRTNSLRKINSNHGIDIRANSLFTEQELLNIYSQELLNRGNLAAASDIVRLLAL
                     KNFGGVYLDVDMLPGIHSDLFKTISRPSSIGLDRWEMIKLEAIMKYKKYINNYTSENF
                     DKLDQQLKDNFKLIIESKSEKSEIFSKLENLNVSDLEIKIAFALGSVINQALISKQGS
                     YLTNLVIEQVKNRYQFLNQHLNPAIESDNNFTDTTKIFHDSLFNSATAENSMFLTKIA
                     PYLQVGFMPEARSTISLSGPGAYASAYYDFINLQENTIEKTLKASDLIEFKFPENNLS
                     QLTEQEINSLWSFDQASAKYQFEKYVRDYTGGSLSEDNGVDFNKNTALDKNYLLNNKI
                     PSNNVEEAGSKNYVHYIIQLQGDDISYEATCNLFSKNPKNSIIIQRNMNESAKSYFLS
                     DDGESILELNKYRIPERLKNKEKVKVTFIGHGKDEFNTSEFARLSVDSLSNEISSFLD
                     TIKLDISPKNVEVNLLGCNMFSYDFNVEETYPGKLLLSIMDKITSTLPDVNKNSITIG
                     ANQYEVRINSEGRKELLAHSGKWINKEEAIMSDLSSKEYIFFDSIDNKLKAKSKNIPG
                     LASISEDIKTLLLDASVSPDTKFILNNLKLNIESSIGDYIYYEKLEPVKNIIHNSIDD
                     LIDEFNLLENVSDELYELKKLNNLDEKYLISFEDISKNNSTYSVRFINKSNGESVYVE
                     TEKEIFSKYSEHITKEISTIKNSIITDVNGNLLDNIQLDHTSQVNTLNAAFFIQSLID
                     YSSNKDVLNDLSTSVKVQLYAQLFSTGLNTIYDSIQLVNLISNAVNDTINVLPTITEG
                     IPIVSTILDGINLGAAIKELLDEHDPLLKKELEAKVGVLAINMSLSIAATVASIVGIG
                     AEVTIFLLPIAGISAGIPSLVNNELILHDKATSVVNYFNHLSESKKYGPLKTEDDKIL
                     VPIDDLVISEIDFNNNSIKLGTCNILAMEGGSGHTVTGNIDHFFSSPSISSHIPSLSI
                     YSAIGIETENLDFSKKIMMLPNAPSRVFWWETGAVPGLRSLENDGTRLLDSIRDLYPG
                     KFYWRFYAFFDYAITTLKPVYEDTNIKIKLDKDTRNFIMPTITTNEIRNKLSYSFDGA
                     GGTYSLLLSSYPISTNINLSKDDLWIFNIDNEVREISIENGTIKKGKLIKDVLSKIDI
                     NKNKLIIGNQTIDFSGDIDNKDRYIFLTCELDDKISLIIEINLVAKSYSLLLSGDKNY
                     LISNLSNTIEKINTLGLDSKNIAYNYTDESNNKYFGAISKTSQKSIIHYKKDSKNILE
                     FYNDSTLEFNSKDFIAEDINVFMKDDINTITGKYYVDNNTDKSIDFSISLVSKNQVKV
                     NGLYLNESVYSSYLDFVKNSDGHHNTSNFMNLFLDNISFWKLFGFENINFVIDKYFTL
                     VGKTNLGYVEFICDNNKNIDIYFGEWKTSSSKSTIFSGNGRNVVVEPIYNPDTGEDIS
                     TSLDFSYEPLYGIDRYINKVLIAPDLYTSLININTNYYSNEYYPEIIVLNPNTFHKKV
                     NINLDSSSFEYKWSTEGSDFILVRYLEESNKKILQKIRIKGILSNTQSFNKMSIDFKD
                     IKKLSLGYIMSNFKSFNSENELDRDHLGFKIIDNKTYYYDEDSKLVKGLININNSLFY
                     FDPIEFNLVTGWQTINGKKYYFDINTGAALTSYKIINGKHFYFNNDGVMQLGVFKGPD
                     GFEYFAPANTQNNNIEGQAIVYQSKFLTLNGKKYYFDNDSKAVTGWRIINNEKYYFNP
                     NNAIAAVGLQVIDNNKYYFNPDTAIISKGWQTVNGSRYYFDTDTAIAFNGYKTIDGKH
                     FYFDSDCVVKIGVFSTSNGFEYFAPANTYNNNIEGQAIVYQSKFLTLNGKKYYFDNNS
                     KAVTGWQTIDSKKYYFNTNTAEAATGWQTIDGKKYYFNTNTAEAATGWQTIDGKKYYF
                     NTNTAIASTGYTIINGKHFYFNTDGIMQIGVFKGPNGFEYFAPANTDANNIEGQAILY
                     QNEFLTLNGKKYYFGSDSKAVTGWRIINNKKYYFNPNNAIAAIHLCTINNDKYYFSYD
                     GILQNGYITIERNNFYFDANNESKMVTGVFKGPNGFEYFAPANTHNNNIEGQAIVYQN
                     KFLTLNGKKYYFDNDSKAVTGWQTIDGKKYYFNLNTAEAATGWQTIDGKKYYFNLNTA
                     EAATGWQTIDGKKYYFNTNTFIASTGYTSINGKHFYFNTDGIMQIGVFKGPNGFEYFA
                     PANTHNNNIEGQAILYQNKFLTLNGKKYYFGSDSKAVTGLRTIDGKKYYFNTNTAVAV
                     TGWQTINGKKYYFNTNTSIASTGYTIISGKHFYFNTDGIMQIGVFKGPDGFEYFAPAN
                     TDANNIEGQAIRYQNRFLYLHDNIYYFGNNSKAATGWVTIDGNRYYFEPNTAMGANGY
                     KTIDNKNFYFRNGLPQIGVFKGSNGFEYFAPANTDANNIEGQAIRYQNRFLHLLGKIY
                     YFGNNSKAVTGWQTINGKVYYFMPDTAMAAAGGLFEIDGVIYFFGVDGVKAPGIYG"
BASE COUNT         7046 a         1888 c         2794 g         5920 t
ORIGIN      
        1 ttgtcaaaat tttcaaataa atcatcatta taaatataag agaggatgat tttatgcaaa
       61 agtcttttta tgaattaatt gttttagcaa gaaataactc agtagatgat ttgcaagaaa
      121 ttttatttat gtttaagcca ttagtaaaaa aacttagtag agttttacat tatgaagagg
      181 gagaaacaga tttaataata ttttttattg aattaataaa aaatattaaa ttaagtagct
      241 tttcagaaaa aagcgatgct attatagtca aatatattca taaatcatta ctgaataaga
      301 cttttgagtt gtctagaaga tattctaaaa tgaagtttaa ttttgtagaa tttgatgaaa
      361 atatcttaaa tatgaaaaat aattatcaaa gtaagtctgt ttttgaggaa gatatttgtt
      421 ttttcgaata tattttgaaa gaattatctg gtattcaaag aaaagttatt ttttataaat
      481 atttaaaagg atattctgat agagaaatat cagtgaaatt aaaaatatct agacaagctg
      541 ttaataaggc taaaaataga gcatttaaaa aaataaaaaa agactatgaa aattatttta
      601 acttgtaatt aatgagctta aagaaatatt tacaatagaa atcaaatttt agaattaact
      661 ttattgtaaa atcaataact taatctaaga atatcttaat ttttatattt tatatagaac
      721 aaagtttaca tatttatttc agacaacgtc tttattcaat cgaagagcaa attaatcaac
      781 tgagtgtctt aaatttaaaa tgttaggaag tgaatgtata tgaaaaccta agtagatatt
      841 agtatatttt ataaatagaa aggaggatat ataaaagagt tttagcattt agatgtaaaa
      901 atattatagt aaaggagaaa attttatgag tttagttaat agaaaacagt tagaaaaaat
      961 ggcaaatgta agatttcgta ctcaagaaga tgaatatgtt gcaatattgg atgctttaga
     1021 agaatatcat aatatgtcag agaatactgt agtcgaaaaa tatttaaaat taaaagatat
     1081 aaatagttta acagatattt atatagatac atataaaaaa tctggtagaa ataaagcctt
     1141 aaaaaaattt aaggaatatc tagttacaga agtattagag ctaaagaata ataatttaac
     1201 tccagttgag aaaaatttac attttgtttg gattggaggt caaataaatg acactgctat
     1261 taattatata aatcaatgga aagatgtaaa tagtgattat aatgttaatg ttttttatga
     1321 tagtaatgca tttttgataa acacattgaa aaaaactgta gtagaatcag caataaatga
     1381 tacacttgaa tcatttagag aaaacttaaa tgaccctaga tttgactata ataaattctt
     1441 cagaaaacgt atggaaataa tttatgataa acagaaaaat ttcataaact actataaagc
     1501 tcaaagagaa gaaaatcctg aacttataat tgatgatatt gtaaagacat atctttcaaa
     1561 tgagtattca aaggagatag atgaacttaa tacctatatt gaagaatcct taaataaaat
     1621 tacacagaat agtggaaatg atgttagaaa ctttgaagaa tttaaaaatg gagagtcatt
     1681 caacttatat gaacaagagt tggtagaaag gtggaattta gctgctgctt ctgacatatt
     1741 aagaatatct gcattaaaag aaattggtgg tatgtattta gatgttgata tgttaccagg
     1801 aatacaacca gacttatttg agtctataga gaaacctagt tcagtaacag tggatttttg
     1861 ggaaatgaca aagttagaag ctataatgaa atacaaagaa tatataccag aatatacctc
     1921 agaacatttt gacatgttag acgaagaagt tcaaagtagt tttgaatctg ttctagcttc
     1981 taagtcagat aaatcagaaa tattctcatc acttggtgat atggaggcat caccactaga
     2041 agttaaaatt gcatttaata gtaagggtat tataaatcaa gggctaattt ctgtgaaaga
     2101 ctcatattgt agcaatttaa tagtaaaaca aatcgagaat agatataaaa tattgaataa
     2161 tagtttaaat ccagctatta gcgaggataa tgattttaat actacaacga atacctttat
     2221 tgatagtata atggctgaag ctaatgcaga taatggtaga tttatgatgg aactaggaaa
     2281 gtatttaaga gttggtttct tcccagatgt taaaactact attaacttaa gtggccctga
     2341 agcatatgcg gcagcttatc aagatttatt aatgtttaaa gaaggcagta tgaatatcca
     2401 tttgatagaa gctgatttaa gaaactttga aatctctaaa actaatattt ctcaatcaac
     2461 tgaacaagaa atggctagct tatggtcatt tgacgatgca agagctaaag ctcaatttga
     2521 agaatataaa aggaattatt ttgaaggttc tcttggtgaa gatgataatc ttgatttttc
     2581 tcaaaatata gtagttgaca aggagtatct tttagaaaaa atatcttcat tagcaagaag
     2641 ttcagagaga ggatatatac actatattgt tcagttacaa ggagataaaa ttagttatga
     2701 agcagcatgt aacttatttg caaagactcc ttatgatagt gtactatttc agaaaaatat
     2761 agaagattca gaaattgcat attattataa tcctggagat ggtgaaatac aagaaataga
     2821 caagtataaa attccaagta taatttctga tagacctaag attaaattaa catttattgg
     2881 tcatggtaaa gatgaattta atactgatat atttgcaggt tttgatgtag attcattatc
     2941 cacagaaata gaagcagcaa tagatttagc taaagaggat atttctccta agtcaataga
     3001 aataaattta ttaggatgta atatgtttag ctactctatc aacgtagagg agacttatcc
     3061 tggaaaatta ttacttaaag ttaaagataa aatatcagaa ttaatgccat ctataagtca
     3121 agactctatt atagtaagtg caaatcaata tgaagttaga ataaatagtg aaggaagaag
     3181 agaattattg gatcattctg gtgaatggat aaataaagaa gaaagtatta taaaggatat
     3241 ttcatcaaaa gaatatatat catttaatcc taaagaaaat aaaattacag taaaatctaa
     3301 aaatttacct gagctatcta cattattaca agaaattaga aataattcta attcaagtga
     3361 tattgaacta gaagaaaaag taatgttaac agaatgtgag ataaatgtta tttcaaatat
     3421 agatacgcaa attgttgagg aaaggattga agaagctaag aatttaactt ctgactctat
     3481 taattatata aaagatgaat ttaaactaat agaatctatt tctgatgcac tatgtgactt
     3541 aaaacaacag aatgaattag aagattctca ttttatatct tttgaggaca tatcagagac
     3601 tgatgaggga tttagtataa gatttattaa taaagaaact ggagaatcta tatttgtaga
     3661 aactgaaaaa acaatattct ctgaatatgc taatcatata actgaagaga tttctaagat
     3721 aaaaggtact atatttgata ctgtaaatgg taagttagta aaaaaagtaa atttagatac
     3781 tacacacgaa gtaaatactt taaatgctgc attttttata caatcattaa tagaatataa
     3841 tagttctaaa gaatctctta gtaatttaag tgtagcaatg aaagtccaag tttacgctca
     3901 attatttagt actggtttaa atactattac agatgcagcc aaagttgttg aattagtatc
     3961 aactgcatta gatgaaacta tagacttact tcctacatta tctgaaggat tacctataat
     4021 tgcaactatt atagatggtg taagtttagg tgcagcaatc aaagagctaa gtgaaacgag
     4081 tgacccatta ttaagacaag aaatagaagc taagataggt ataatggcag taaatttaac
     4141 aacagctaca actgcaatca ttacttcatc tttgggggta gctagtggat ttagtatact
     4201 tttagttcct ttagcaggaa tttcagcagg tataccaagc ttagtaaaca atgaacttgt
     4261 acttcgagat aaggcaacaa aggttgtaga ttattttaaa catgtttcat tagttgaaac
     4321 tgaaggagta tttactttat tagatgataa aataatgatg ccacaagatg atttagtgat
     4381 atcagaaata gattttaata ataattcaat agttttaggt aaatgtgaaa tctggagaat
     4441 ggaaggtggt tcaggtcata ctgtaactga tgatatagat cacttctttt cagcaccatc
     4501 aataacatat agagagccac acttatctat atatgacgta ttggaagtac aaaaagaaga
     4561 acttgatttg tcaaaagatt taatggtatt acctaatgct ccaaatagag tatttgcttg
     4621 ggaaacagga tggacaccag gtttaagaag cttagaaaat gatggcacaa aactgttaga
     4681 ccgtataaga gataactatg aaggtgagtt ttattggaga tattttgctt ttatagctga
     4741 tgctttaata acaacattaa aaccaagata tgaagatact aatataagaa taaatttaga
     4801 tagtaatact agaagtttta tagttccaat aataactaca gaatatataa gagaaaaatt
     4861 atcatattct ttctatggtt caggaggaac ttatgcattg tctctttctc aatataatat
     4921 gggtataaat atagaattaa gtgaaagtga tgtttggatt atagatgttg ataatgttgt
     4981 gagagatgta actatagaat ctgataaaat taaaaaaggt gatttaatag aaggtatttt
     5041 atctacacta agtattgaag agaataaaat tatcttaaat agccatgaga ttaatttttc
     5101 tggtgaggta aatggaagta atggatttgt ttctttaaca ttttcaattt tagaaggaat
     5161 aaatgcaatt atagaagttg atttattatc taaatcatat aaattactta tttctggcga
     5221 attaaaaata ttgatgttaa attcaaatca tattcaacag aaaatagatt atataggatt
     5281 caatagcgaa ttacagaaaa atataccata tagctttgta gatagtgaag gaaaagagaa
     5341 tggttttatt aatggttcaa caaaagaagg tttatttgta tctgaattac ctgatgtagt
     5401 tcttataagt aaggtttata tggatgatag taagccttca tttggatatt atagtaataa
     5461 tttgaaagat gtcaaagtta taactaaaga taatgttaat atattaacag gttattatct
     5521 taaggatgat ataaaaatct ctctttcttt gactctacaa gatgaaaaaa ctataaagtt
     5581 aaatagtgtg catttagatg aaagtggagt agctgagatt ttgaagttca tgaatagaaa
     5641 aggtaataca aatacttcag attctttaat gagcttttta gaaagtatga atataaaaag
     5701 tattttcgtt aatttcttac aatctaatat taagtttata ttagatgcta attttataat
     5761 aagtggtact acttctattg gccaatttga gtttatttgt gatgaaaatg ataatataca
     5821 accatatttc attaagttta atacactaga aactaattat actttatatg taggaaatag
     5881 acaaaatatg atagtggaac caaattatga tttagatgat tctggagata tatcttcaac
     5941 tgttatcaat ttctctcaaa agtatcttta tggaatagac agttgtgtta ataaagttgt
     6001 aatttcacca aatatttata cagatgaaat aaatataacg cctgtatatg aaacaaataa
     6061 tacttatcca gaagttattg tattagatgc aaattatata aatgaaaaaa taaatgttaa
     6121 tatcaatgat ctatctatac gatatgtatg gagtaatgat ggtaatgatt ttattcttat
     6181 gtcaactagt gaagaaaata aggtgtcaca agttaaaata agattcgtta atgtttttaa
     6241 agataagact ttggcaaata agctatcttt taactttagt gataaacaag atgtacctgt
     6301 aagtgaaata atcttatcat ttacaccttc atattatgag gatggattga ttggctatga
     6361 tttgggtcta gtttctttat ataatgagaa attttatatt aataactttg gaatgatggt
     6421 atctggatta atatatatta atgattcatt atattatttt aaaccaccag taaataattt
     6481 gataactgga tttgtgactg taggcgatga taaatactac tttaatccaa ttaatggtgg
     6541 agctgcttca attggagaga caataattga tgacaaaaat tattatttca accaaagtgg
     6601 agtgttacaa acaggtgtat ttagtacaga agatggattt aaatattttg ccccagctaa
     6661 tacacttgat gaaaacctag aaggagaagc aattgatttt actggaaaat taattattga
     6721 cgaaaatatt tattattttg atgataatta tagaggagct gtagaatgga aagaattaga
     6781 tggtgaaatg cactatttta gcccagaaac aggtaaagct tttaaaggtc taaatcaaat
     6841 aggtgattat aaatactatt tcaattctga tggagttatg caaaaaggat ttgttagtat
     6901 aaatgataat aaacactatt ttgatgattc tggtgttatg aaagtaggtt acactgaaat
     6961 agatggcaag catttctact ttgctgaaaa cggagaaatg caaataggag tatttaatac
     7021 agaagatgga tttaaatatt ttgctcatca taatgaagat ttaggaaatg aagaaggtga
     7081 agaaatctca tattctggta tattaaattt caataataaa atttactatt ttgatgattc
     7141 atttacagct gtagttggat ggaaagattt agaggatggt tcaaagtatt attttgatga
     7201 agatacagca gaagcatata taggtttgtc attaataaat gatggtcaat attattttaa
     7261 tgatgatgga attatgcaag ttggatttgt cactataaat gataaagtct tctacttctc
     7321 tgactctgga attatagaat ctggagtaca aaacatagat gacaattatt tctatataga
     7381 tgataatggt atagttcaaa ttggtgtatt tgatacttca gatggatata aatattttgc
     7441 acctgctaat actgtaaatg ataatattta cggacaagca gttgaatata gtggtttagt
     7501 tagagttggg gaagatgtat attattttgg agaaacatat acaattgaga ctggatggat
     7561 atatgatatg gaaaatgaaa gtgataaata ttatttcaat ccagaaacta aaaaagcatg
     7621 caaaggtatt aatttaattg atgatataaa atattatttt gatgagaagg gcataatgag
     7681 aacgggtctt atatcatttg aaaataataa ttattacttt aatgagaatg gtgaaatgca
     7741 atttggttat ataaatatag aagataagat gttctatttt ggtgaagatg gtgtcatgca
     7801 gattggagta tttaatacac cagatggatt taaatacttt gcacatcaaa atactttgga
     7861 tgagaatttt gagggagaat caataaacta tactggttgg ttagatttag atgaaaagag
     7921 atattatttt acagatgaat atattgcagc aactggttca gttattattg atggtgagga
     7981 gtattatttt gatcctgata cagctcaatt agtgattagt gaatagataa aaatatgtta
     8041 aatatatcct cttatactta aatatataaa aataaacaaa atgatacact acataaagtg
     8101 ttctatctaa tatgaagatt taccaataaa aaggtggact atgatgaatg cacagtagtt
     8161 caccttttta tatttctaat ggtaacaaaa tattttttta tataaaccta ggaggcgtta
     8221 tgaatatgac aatatctttt ttatcagagc atatatttat aaagttagta attttaacta
     8281 tatcatttga tacattatta ggatgtttaa gtgcaataaa aagtcgtaaa tttaattcta
     8341 gttttggaat agatggagga atcagaaaag tagcaatgat agcatgtata ttttttttat
     8401 cagtagttga cattcttaca aagtttaact ttttatttat gttaccacaa gattgtatca
     8461 attttttaag actaaaacat cttggaatat ctgaattttt ctctatttta tttattttat
     8521 atgaaagtgt aagtatatta aaaaatatgt gcttatgtgg attaccagta cctaagagat
     8581 taaaggaaaa aatagcaatt ttactagatg caatgacaga tgaaatgaat gctaaggatg
     8641 aaaagtaagt aatggtagat ataataaaga tattaacaaa taaaaagtgt tatccaaata
     8701 agaatagctg aaagttatca taattcatga aactaataat gaaaacgagg gagcagatgc
     8761 caagagacac acaagtatta aatacatata atttcgaagc aagtgttcat tactatatag
     8821 atgacaaggt agtatatcaa acattggttc acaaagatgg tgcatggtca gttggtaaaa
     8881 tctattaagc tacattagtt acagatatca caaactataa tagttaaaca tagaaatatg
     8941 tgtaaattgt gatggaaatt attcaaaaac acaaaaatac gtgatgaagg acaaaatgat
     9001 atagaaaata agtatcaaac cttaataaat gatttaattg atagtttaaa agttatagga
     9061 aaaatatata aagaaataaa aacattaaaa aaatataaga tatgtttaca aattactatc
     9121 agacaatctc cttatctaat agaagagtca attaactaat tgagtatctt taaattgaaa
     9181 tgttaggaag tgatttaaat atgaaaactt aaattataaa aaatcaatat taatttattt
     9241 ttaaaaaata gaaaggagtg tataagattt attttcaaag tttaaaaaca agaaaatcaa
     9301 tttaaatttc agaaggaata aatgtggtta tagaagtgga tttattatca aaaataataa
     9361 tactaggagg tttttatgtc tttaatatct aaagaagagt taataaaact cgcatatagc
     9421 attagaccaa gagaaaatga gtataaaact atactaacta atttagacga atataataag
     9481 ttaactacaa acaataatga aaataaatat ttacaattaa aaaaactaaa tgaatcaatt
     9541 gatgttttta tgaataaata taaaacttca agcagaaata gagcactctc taatctaaaa
     9601 aaagatatat taaaagaagt aattcttatt aaaaattcca atacaagccc tgtagaaaaa
     9661 aatttacatt ttgtatggat aggtggagaa gtcagtgata ttgctcttga atacataaaa
     9721 caatgggctg atattaatgc agaatataat attaaactgt ggtatgatag tgaagcattc
     9781 ttagtaaata cactaaaaaa ggctatagtt gaatcttcta ccactgaagc attacagcta
     9841 ctagaggaag agattcaaaa tcctcaattt gataatatga aattttacaa aaaaaggatg
     9901 gaatttatat atgatagaca aaaaaggttt ataaattatt ataaatctca aatcaataaa
     9961 cctacagtac ctacaataga tgatattata aagtctcatc tagtatctga atataataga
    10021 gatgaaactg tattagaatc atatagaaca aattctttga gaaaaataaa tagtaatcat
    10081 gggatagata tcagggctaa tagtttgttt acagaacaag agttattaaa tatttatagt
    10141 caggagttgt taaatcgtgg aaatttagct gcagcatctg acatagtaag attattagcc
    10201 ctaaaaaatt ttggcggagt atatttagat gttgatatgc ttccaggtat tcactctgat
    10261 ttatttaaaa caatatctag acctagctct attggactag accgttggga aatgataaaa
    10321 ttagaggcta ttatgaagta taaaaaatat ataaataatt atacatcaga aaactttgat
    10381 aaacttgatc aacaattaaa agataatttt aaactcatta tagaaagtaa aagtgaaaaa
    10441 tctgagatat tttctaaatt agaaaattta aatgtatctg atcttgaaat taaaatagct
    10501 ttcgctttag gcagtgttat aaatcaagcc ttgatatcaa aacaaggttc atatcttact
    10561 aacctagtaa tagaacaagt aaaaaataga tatcaatttt taaaccaaca ccttaaccca
    10621 gccatagagt ctgataataa cttcacagat actactaaaa tttttcatga ttcattattt
    10681 aattcagcta ccgcagaaaa ctctatgttt ttaacaaaaa tagcaccata cttacaagta
    10741 ggttttatgc cagaagctcg ctccacaata agtttaagtg gtccaggagc ttatgcgtca
    10801 gcttactatg atttcataaa tttacaagaa aatactatag aaaaaacttt aaaagcatca
    10861 gatttaatag aatttaaatt cccagaaaat aatctatctc aattgacaga acaagaaata
    10921 aatagtctat ggagctttga tcaagcaagt gcaaaatatc aatttgagaa atatgtaaga
    10981 gattatactg gtggatctct ttctgaagac aatggggtag actttaataa aaatactgcc
    11041 ctcgacaaaa actatttatt aaataataaa attccatcaa acaatgtaga agaagctgga
    11101 agtaaaaatt atgttcatta tatcatacag ttacaaggag atgatataag ttatgaagca
    11161 acatgcaatt tattttctaa aaatcctaaa aatagtatta ttatacaacg aaatatgaat
    11221 gaaagtgcaa aaagctactt tttaagtgat gatggagaat ctattttaga attaaataaa
    11281 tataggatac ctgaaagatt aaaaaataag gaaaaagtaa aagtaacctt tattggacat
    11341 ggtaaagatg aattcaacac aagcgaattt gctagattaa gtgtagattc actttccaat
    11401 gagataagtt catttttaga taccataaaa ttagatatat cacctaaaaa tgtagaagta
    11461 aacttacttg gatgtaatat gtttagttat gattttaatg ttgaagaaac ttatcctggg
    11521 aagttgctat taagtattat ggacaaaatt acttccactt tacctgatgt aaataaaaat
    11581 tctattacta taggagcaaa tcaatatgaa gtaagaatta atagtgaggg aagaaaagaa
    11641 cttctggctc actcaggtaa atggataaat aaagaagaag ctattatgag cgatttatct
    11701 agtaaagaat acattttttt tgattctata gataataagc taaaagcaaa gtccaagaat
    11761 attccaggat tagcatcaat atcagaagat ataaaaacat tattacttga tgcaagtgtt
    11821 agtcctgata caaaatttat tttaaataat cttaagctta atattgaatc ttctattggt
    11881 gattacattt attatgaaaa attagagcct gttaaaaata taattcacaa ttctatagat
    11941 gatttaatag atgagttcaa tctacttgaa aatgtatctg atgaattata tgaattaaaa
    12001 aaattaaata atctagatga gaagtattta atatcttttg aagatatctc aaaaaataat
    12061 tcaacttact ctgtaagatt tattaacaaa agtaatggtg agtcagttta tgtagaaaca
    12121 gaaaaagaaa ttttttcaaa atatagcgaa catattacaa aagaaataag tactataaag
    12181 aatagtataa ttacagatgt taatggtaat ttattggata atatacagtt agatcatact
    12241 tctcaagtta atacattaaa cgcagcattc tttattcaat cattaataga ttatagtagc
    12301 aataaagatg tactgaatga tttaagtacc tcagttaagg ttcaacttta tgctcaacta
    12361 tttagtacag gtttaaatac tatatatgac tctatccaat tagtaaattt aatatcaaat
    12421 gcagtaaatg atactataaa tgtactacct acaataacag aggggatacc tattgtatct
    12481 actatattag acggaataaa cttaggtgca gcaattaagg aattactaga cgaacatgac
    12541 ccattactaa aaaaagaatt agaagctaag gtgggtgttt tagcaataaa tatgtcatta
    12601 tctatagctg caactgtagc ttcaattgtt ggaataggtg ctgaagttac tattttctta
    12661 ttacctatag ctggtatatc tgcaggaata ccttcattag ttaataatga attaatattg
    12721 catgataagg caacttcagt ggtaaactat tttaatcatt tgtctgaatc taaaaaatat
    12781 ggccctctta agacagaaga tgataaaatt ttagttccta ttgatgattt agtaatatca
    12841 gaaatagatt ttaataataa ttcgataaaa ctaggaacat gtaatatatt agcaatggag
    12901 gggggatcag gacacacagt gactggtaat atagatcact ttttctcatc tccatctata
    12961 agttctcata ttccttcatt atcaatttat tctgcaatag gtatagaaac agaaaatcta
    13021 gatttttcaa aaaaaataat gatgttacct aatgctcctt caagagtgtt ttggtgggaa
    13081 actggagcag ttccaggttt aagatcattg gaaaatgacg gaactagatt acttgattca
    13141 ataagagatt tatacccagg taaattttac tggagattct atgctttttt cgattatgca
    13201 ataactacat taaaaccagt ttatgaagac actaatatta aaattaaact agataaagat
    13261 actagaaact tcataatgcc aactataact actaacgaaa ttagaaacaa attatcttat
    13321 tcatttgatg gagcaggagg aacttactct ttattattat cttcatatcc aatatcaacg
    13381 aatataaatt tatctaaaga tgatttatgg atatttaata ttgataatga agtaagagaa
    13441 atatctatag aaaatggtac tattaaaaaa ggaaagttaa taaaagatgt tttaagtaaa
    13501 attgatataa ataaaaataa acttattata ggcaatcaaa caatagattt ttcaggcgat
    13561 atagataata aagatagata tatattcttg acttgtgagt tagatgataa aattagttta
    13621 ataatagaaa taaatcttgt tgcaaaatct tatagtttgt tattgtctgg ggataaaaat
    13681 tatttgatat ccaatttatc taatactatt gagaaaatca atactttagg cctagatagt
    13741 aaaaatatag cgtacaatta cactgatgaa tctaataata aatattttgg agctatatct
    13801 aaaacaagtc aaaaaagcat aatacattat aaaaaagaca gtaaaaatat attagaattt
    13861 tataatgaca gtacattaga atttaacagt aaagatttta ttgctgaaga tataaatgta
    13921 tttatgaaag atgatattaa tactataaca ggaaaatact atgttgataa taatactgat
    13981 aaaagtatag atttctctat ttctttagtt agtaaaaatc aagtaaaagt aaatggatta
    14041 tatttaaatg aatccgtata ctcatcttac cttgattttg tgaaaaattc agatggacac
    14101 cataatactt ctaattttat gaatttattt ttggacaata taagtttctg gaaattgttt
    14161 gggtttgaaa atataaattt tgtaatcgat aaatacttta cccttgttgg taaaactaat
    14221 cttggatatg tagaatttat ttgtgacaat aataaaaata tagatatata ttttggtgaa
    14281 tggaaaacat cgtcatctaa aagcactata tttagcggaa atggtagaaa tgttgtagta
    14341 gagcctatat ataatcctga tacgggtgaa gatatatcta cttcactaga tttttcctat
    14401 gaacctctct atggaataga tagatatatc aataaagtat tgatagcacc tgatttatat
    14461 acaagtttaa taaatattaa taccaattat tattcaaatg agtactaccc tgagattata
    14521 gttcttaacc caaatacatt ccacaaaaaa gtaaatataa atttagatag ttcttctttt
    14581 gagtataaat ggtctacaga aggaagtgac tttattttag ttagatactt agaagaaagt
    14641 aataaaaaaa tattacaaaa aataagaatc aaaggtatct tatctaatac tcaatcattt
    14701 aataaaatga gtatagattt taaagatatt aaaaaactat cattaggata tataatgagt
    14761 aattttaaat catttaattc tgaaaatgaa ttagatagag atcatttagg atttaaaata
    14821 atagataata aaacttatta ctatgatgaa gatagtaaat tagttaaagg attaatcaat
    14881 ataaataatt cattattcta ttttgatcct atagaattta acttagtaac tggatggcaa
    14941 actatcaatg gtaaaaaata ttattttgat ataaatactg gagcagcttt aactagttat
    15001 aaaattatta atggtaaaca cttttatttt aataatgatg gtgtgatgca gttgggagta
    15061 tttaaaggac ctgatggatt tgaatatttt gcacctgcca atactcaaaa taataacata
    15121 gaaggtcagg ctatagttta tcaaagtaaa ttcttaactt tgaatggcaa aaaatattat
    15181 tttgataatg actcaaaagc agtcactgga tggagaatta ttaacaatga gaaatattac
    15241 tttaatccta ataatgctat tgctgcagtc ggattgcaag taattgacaa taataagtat
    15301 tatttcaatc ctgacactgc tatcatctca aaaggttggc agactgttaa tggtagtaga
    15361 tactactttg atactgatac cgctattgcc tttaatggtt ataaaactat tgatggtaaa
    15421 cacttttatt ttgatagtga ttgtgtagtg aaaataggtg tgtttagtac ctctaatgga
    15481 tttgaatatt ttgcacctgc taatacttat aataataaca tagaaggtca ggctatagtt
    15541 tatcaaagta aattcttaac tttgaatggt aaaaaatatt actttgataa taactcaaaa
    15601 gcagttaccg gatggcaaac tattgatagt aaaaaatatt actttaatac taacactgct
    15661 gaagcagcta ctggatggca aactattgat ggtaaaaaat attactttaa tactaacact
    15721 gctgaagcag ctactggatg gcaaactatt gatggtaaaa aatattactt taatactaac
    15781 actgctatag cttcaactgg ttatacaatt attaatggta aacattttta ttttaatact
    15841 gatggtatta tgcagatagg agtgtttaaa ggacctaatg gatttgaata ttttgcacct
    15901 gctaatacgg atgctaacaa catagaaggt caagctatac tttaccaaaa tgaattctta
    15961 actttgaatg gtaaaaaata ttactttggt agtgactcaa aagcagttac tggatggaga
    16021 attattaaca ataagaaata ttactttaat cctaataatg ctattgctgc aattcatcta
    16081 tgcactataa ataatgacaa gtattacttt agttatgatg gaattcttca aaatggatat
    16141 attactattg aaagaaataa tttctatttt gatgctaata atgaatctaa aatggtaaca
    16201 ggagtattta aaggacctaa tggatttgag tattttgcac ctgctaatac tcacaataat
    16261 aacatagaag gtcaggctat agtttaccag aacaaattct taactttgaa tggcaaaaaa
    16321 tattattttg ataatgactc aaaagcagtt actggatggc aaaccattga tggtaaaaaa
    16381 tattacttta atcttaacac tgctgaagca gctactggat ggcaaactat tgatggtaaa
    16441 aaatattact ttaatcttaa cactgctgaa gcagctactg gatggcaaac tattgatggt
    16501 aaaaaatatt actttaatac taacactttc atagcctcaa ctggttatac aagtattaat
    16561 ggtaaacatt tttattttaa tactgatggt attatgcaga taggagtgtt taaaggacct
    16621 aatggatttg aatactttgc acctgctaat actcataata ataacataga aggtcaagct
    16681 atactttacc aaaataaatt cttaactttg aatggtaaaa aatattactt tggtagtgac
    16741 tcaaaagcag ttaccggatt gcgaactatt gatggtaaaa aatattactt taatactaac
    16801 actgctgttg cagttactgg atggcaaact attaatggta aaaaatacta ctttaatact
    16861 aacacttcta tagcttcaac tggttataca attattagtg gtaaacattt ttattttaat
    16921 actgatggta ttatgcagat aggagtgttt aaaggacctg atggatttga atactttgca
    16981 cctgctaata cagatgctaa caatatagaa ggtcaagcta tacgttatca aaatagattc
    17041 ctatatttac atgacaatat atattatttt ggtaataatt caaaagcggc tactggttgg
    17101 gtaactattg atggtaatag atattacttc gagcctaata cagctatggg tgcgaatggt
    17161 tataaaacta ttgataataa aaatttttac tttagaaatg gtttacctca gataggagtg
    17221 tttaaagggt ctaatggatt tgaatacttt gcacctgcta atacggatgc taacaatata
    17281 gaaggtcaag ctatacgtta tcaaaataga ttcctacatt tacttggaaa aatatattac
    17341 tttggtaata attcaaaagc agttactgga tggcaaacta ttaatggtaa agtatattac
    17401 tttatgcctg atactgctat ggctgcagct ggtggacttt tcgagattga tggtgttata
    17461 tatttctttg gtgttgatgg agtaaaagcc cctgggatat atggctaaaa tatatgtttg
    17521 ataaaaaatt attcctgtgc tactaagaaa ttatttttat ataataaata ttgagattta
    17581 attaaaagtc atgtgttatt gtaatacatg acttttagtt aaaatttttc tatcatttaa
    17641 taatctat
//
LOCUS       DBGJFF010000077        17573 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.078, whole genome shotgun sequence.
ACCESSION   DBGJFF010000077 DBGJFF010000000
VERSION     DBGJFF010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17573
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.078"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            381..2258
                     /locus_tag="ACOIAF_002519"
     CDS             381..2258
                     /locus_tag="ACOIAF_002519"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HGZ2669525.1"
                     /translation="MDYKNIAQEILLNVGGKENVNEVTHCMTRLRFKVKSASKVNKDK
                     LSKTEGVITVVESMGQIQVVIGNKVKKVYDEVIKIVPQSNNIGKKNESQEKQGIINSI
                     LSAVAGIFTPTIPAIAGVGMIKGILSVLAMYYMNKNGVDIKETQSYIILNAMADSIFY
                     FMPIILGYTAAKVFNANKIISMVLGATLCYPTFTALMAGEESVRFLGLAVTKATYTSS
                     VIPIIIAIWALSYVEKVLEKYIPEVIKIIMVPTLSLVVMLPATLFLFGPIGIYIGNVI
                     NFSYKYIYELSPALCGAFVGGLWCVLVIFGAHRALLPIGISDVAQTGRQNLLAFAGAA
                     NFSQAGAALGVFFKTKNQGLKTISMSATITALFGITEPAIYGANLRLKKPMVCAVICG
                     AFGGAIMGMGGAYGNAFANQGVLTIPVYAEAGALGFLSYLGGCAIAFFGSAISTYLVG
                     FEDLEESSNKENSSIKVETKDGVIDITSPVEGECIELSEVKDDVFASKAMGEGIAVLP
                     TKGVITAPTDCEVASLFPTLHAIGLKLDNGAEMLIHVGINTVELNGKYFTKHVNQGDL
                     VKKGDKLISFDIDKIKKAGYDVTTPVIVNNTFDFGQVISCKSSYVSTNDNIISLVLAG
                     N"
     gene            2570..3976
                     /locus_tag="ACOIAF_002520"
     CDS             2570..3976
                     /locus_tag="ACOIAF_002520"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HGZ2669526.1"
                     /translation="MNTGFPKDFLWGASSSAFQVEGAWDKDNKGKTVADYNSFKKSHL
                     QADTKVASDFYHNYEEDIELMKELGMKTYRFSISWARIIPDGEGEINQKGLDFYNKII
                     DKLIECDIEPFVTLYHFDLPFKLVEKYNGWESRETVYAFERFAKICFKHFGDRVKYWQ
                     PHNEQNLIVRVEERINIYDETDSWKIDKMRAQMDYNLCLAHALAVNACHEMIKESKIG
                     AAVSSSVTYPLTSKPEDVYAARMNDNFKVYYMLDMHHYGEYPGYYMKYLEKRNIVPHM
                     EDGDKEILKKAKMDFIAVNYYRTNCAEALPEDSQHPFGLREGTVDFSMYGLFKMSMNP
                     NLEASEYGAAIDPSGLRVALNEYWQRYHLPVIITENGLGAKDILEDGKIHDDYRIDYL
                     RSHINACKLAIEDGVEMLGYCPWSFTDLLSSSQGFNKRYGLVYINRTDHEVLDLKRIK
                     KDSFYWYKEVIENNGIVK"
     gene            4004..4852
                     /locus_tag="ACOIAF_002521"
     CDS             4004..4852
                     /locus_tag="ACOIAF_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HGZ2669527.1"
                     /translation="MYITKVLNNSLLLAKDDNDEEIILMGKGIGHNYKAGSELNKKDI
                     EKIFVLHDENIKKSIIQLATEIDAEYFGIAQMIIAYGIEKYNLKLMNHIYLALTDHIA
                     FAVRRFKEGIMIENHYLFEIKEFNPKEYDIGKYAITVFKEVLGLDLPEEEIGNIAGHF
                     INAQQDNPYSDRNKRSAKIVNAILQIVHYHFSIVYNKESFYYRRFVMHLKAFSQRFLA
                     NEPSREKIDFIYEQVQKNCKEEYECVKKVGAYMYKEYSRELPRQEELYLMIHIHKILG
                     ELCENS"
     gene            5513..6204
                     /locus_tag="ACOIAF_002522"
                     /pseudo
     CDS             5513..6204
                     /locus_tag="ACOIAF_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728879.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            6201..7136
                     /locus_tag="ACOIAF_002523"
     CDS             6201..7136
                     /locus_tag="ACOIAF_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HGZ2669528.1"
                     /translation="MKMDEQTIILFLLLLAIGLLFLSIFAFSKLGHIYKRLKDIEEIL
                     ADVQIGDENRKILIKPCDVMAPLVYQLNEIVYDYENKLLSLKKSDKASKQLMTSLSHD
                     VRTPLTTLIGYLNAVHSGIVIEQEREEYLEIARRRAYDLKDYIDVLFDWFRLNSDEFT
                     LSIESVEIAELSRNILKDWIPIFHEKKLNFEIDIPENRLMVNLDPDGYSRVVNNLVQN
                     VLAHSKARQIKITMSEDSRMVLLRVEDDGVGIARENLPHIFERLYKCDKGRSEKGSGL
                     GLSIVSQMVERMGGQISVESEIGKYTVFTVSLPLI"
     gene            7284..8207
                     /locus_tag="ACOIAF_002524"
     CDS             7284..8207
                     /locus_tag="ACOIAF_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2669529.1"
                     /translation="MNNLIIETKNLTKQYGAQKSVSNLNIHVQKGRIYGLLGRNGAGK
                     TTTMKMLLGLTKPTTGEIKIFGQDIHNNEKKLLPRIGCLIESPGFYPNLTGTENLKIF
                     ARLRGIPKKNAVESALDVVGLPNNDKKLFSQYSLGMKQRLAIALAIMHDPQLLILDEP
                     INGLDPIGIAEVRTFIRDLCTERGKTILISSHILSEIALLADDIGIIDHGILLEEESL
                     AQLEEKNSKYIHFAVSDAAQAAMLLETQLNLKNFKVADNYNIMLYDTDVSVAEINRAF
                     ILNNIEVSESHLCEDTLEDYFKKVTGGEGIA"
     gene            8191..8961
                     /locus_tag="ACOIAF_002525"
     CDS             8191..8961
                     /locus_tag="ACOIAF_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2669530.1"
                     /translation="MKELLDIISCEFSKLKRLKFILISILGACLFPIPATILIARDNL
                     PFEQLFKLVVNFGYFLLLPIVLSIVASQLFFIERDNDVLKNLATVPVQKGKLALAKLA
                     VLLFISLFYSVAGLGATIIGGFIVGIVEGVAVKLGMSIAFGIMFFVTVLPVVVLIVYF
                     NRSYIFSIILSFVYAIFNFSISLNIINFEPNNPLLSVLPAPVIMRWWMAFWGDPTGEY
                     TVLRQPYLLSTPACVGILCLITIIAVLLICMIYKKQEN"
     gene            8971..9753
                     /locus_tag="ACOIAF_002526"
     CDS             8971..9753
                     /locus_tag="ACOIAF_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2669531.1"
                     /translation="MLDIITTEFQKIKRYNILWIGVVAVLFSALLAVFQVVSSHGSDP
                     LTYESFANGVIWNNFSLSFPFGITLIGGYLINREYTDQTLKNILTVPISLRKLLVGKL
                     IAVGGITVLLSLFSFLCTFILGLLFCNINISVILVVKSLVQILSVNLYCYVAVLPIIA
                     YFGRKQNAFLTGVGIAFVYGFCGVFVAGRNLTDFYPITAGLGLVGYNNGAGTVYQPLI
                     GAMTLIVILILTTILLVFTPNYDKVMTISKKKGIKHKKYSHK"
     gene            10130..10666
                     /gene="rbr"
                     /locus_tag="ACOIAF_002527"
     CDS             10130..10666
                     /gene="rbr"
                     /locus_tag="ACOIAF_002527"
                     /EC_number="1.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016692 - NADH peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin"
                     /protein_id="HGZ2669532.1"
                     /translation="MNLKGTKTEKNLMAGFAGESEARNKYTYYASKARKEGYNQIAAI
                     FEETANNEKEHAKLWFKLLHDGMPSTEENLKDAAAGENYEWTDMYAKFAKEAREEGFD
                     KIAYLFEAVGKIEKEHEERYLKLLENLNEGKIFKRDEEVVWQCQNCGHVYVGTEAPEK
                     CPVCDHPKAYFNIKAENY"
     gene            10698..11087
                     /locus_tag="ACOIAF_002528"
     CDS             10698..11087
                     /locus_tag="ACOIAF_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HGZ2669533.1"
                     /translation="MKFSKQRELILNEILNNPVHPTADYLYENLKKDNPNLSLGTVYR
                     NLAQLTEHGFIRKVSIPGYPDRFDGRIDNHYHIICEVCGEVYDLESEVLNNLQELISE
                     ETDIKITSYNISFKGICNNCKRCSQVG"
     gene            11124..11510
                     /locus_tag="ACOIAF_002529"
     CDS             11124..11510
                     /locus_tag="ACOIAF_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin family protein"
                     /protein_id="HGZ2669534.1"
                     /translation="MCSEQKFFICETCGNLVGMIQSGGVPIFCCGKPMKELVPNTTDA
                     AVEKHVPVIEVDGNNVTVKVSSTTHPMTKEHHIAWVYLMTEQGGQRKCLAVDGEPVVK
                     FALNDDDKVISAYAYCNLHGLWKAEL"
     gene            11637..13079
                     /locus_tag="ACOIAF_002530"
     CDS             11637..13079
                     /locus_tag="ACOIAF_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HGZ2669535.1"
                     /translation="MSIYKCSVCGYIYDESKNDKTWDELSEDWECPVCTKGRSYFGKI
                     STVYYEEDEKIAEDIVEDESKLNTEKEGDLNYLSTYLRRDDEVEKHMDIIHEMAVTGK
                     SIIEPMRTKLPVISWDDILIMGAQLNPLPLNEHDEVNTTTIIGKKAKKPMIIENPVYI
                     SHMSFGALSKELKIALAKGAAQNKTAMCSGEGGILPEEKEASYKYIFEYVPNKYSVTE
                     ENLKNSDAIEIKIGQGTKPGMGGHLPGEKVTEEIAKVRNKPVGQDVISPSCFEEIQSK
                     EDLKKLVDELREVSEGRPIGVKISAGHIEKDMEFIAYAKPDFVTIDGRGGATGASPKL
                     LKDATSIPTIFALYRARKYIDTHGLDIDLVITGGLRISTDFAKAIAMGADAVAIASSA
                     LMAAACQQYRICGSGKCPVGVATQDEELRKRLHIENSANRVANFLNVSLEELKTFARI
                     SGHKDIHDLSVDDLYTVNSEISNYTNIQHV"
     gene            13376..14191
                     /locus_tag="ACOIAF_002531"
     CDS             13376..14191
                     /locus_tag="ACOIAF_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HGZ2669536.1"
                     /translation="MERLKIQVLVDNNTYIDRYFVGEPAVSYYIEIDGNRILFDSGYS
                     DVFISNAEKLNIDLGNLTHVVFSHGHNDHTRGIQFLENRYDLSTVELVSHPNCFIPRK
                     HGEKSIGAPFSAEEIKNIFMYNPKDKPFNLSKNCVFLGEIPSINDFEKRAKIGKCKIG
                     DLWEDDYVLDDSAIVCKTDKGIFIVTGCSHSGICNIVEYAKKVCGDDRVIGILGGFHL
                     FELNNRLDSTIQYLDKEEIRMLYPCHCVSFKVKAKMNEILNINEVGVGLTISI"
     gene            14793..15977
                     /locus_tag="ACOIAF_002532"
     CDS             14793..15977
                     /locus_tag="ACOIAF_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669537.1"
                     /translation="MQKSVKKAKVTGSMLAIFGVASVLFSSHAGGGFATGNQETQYYV
                     QYGWTAPLMAILAMIILTATMREVIIMYNNNNCRNYKDLFCELWRPYPKLEIIWEIYY
                     YLMVLIAVSAVIAGAAAVFQSIGVNYFVAVFIIGVVLLVFTIFGAMLVSKAATAMTIA
                     ILVCTLTIFIVGIKAKVPEITEILSNRTSFTPGYIKPILNTFIYAGFQSVVIPTLAGC
                     SRPLLKNSKEATKAMIISFVMNAIALGLAVTMLMGWYREIIAAGQTTLPTLYVAGQSG
                     NHTIYIIYNVALFLCLMSTGVTTIFGLVNRFEDHKALSFLSSRMKRRVFTACAIMVVS
                     MLISLTGLSNIVKYGYGYCGYLGLFTIVIPFLTLGHYKNKKFAKENPEAKWPAELNEN
                     VN"
     gene            complement(16354..17481)
                     /locus_tag="ACOIAF_002533"
     CDS             complement(16354..17481)
                     /locus_tag="ACOIAF_002533"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HGZ2669538.1"
                     /translation="MKGKITMDFKSLGISENTINILKKSGITTPTPIQKESIKLIKEG
                     KDVIAEAQTGTGKTLAFLLPIFENISLDINDIQVLILSPTRELAIQITEEAMKLKESK
                     DVSILAAYGGKDIGSQIKKLKGNIHMIIATPGRLLDHLNRKTIDLSKLKTFVLDEADQ
                     MLLMGFKNEVEAILKETSNKKQTLCFSATINSQVKKLAYRYTKNPVVVSIQKEEITLN
                     NIKQEVVETTDRKKLDALCKVLDEDNPFMAIIFCRTKRRVDNLEEALAIRGYNCQKLH
                     SDIAQSKRERIMKSFRNLDIQYLIATDVASRGLDISGVSHIYNYDLPETPEDYIHRIG
                     RTGRAGEEGYTCAFIDPKNERMLSEIETAIESKISRRIIEL"
BASE COUNT         6295 a         2182 c         3165 g         5931 t
ORIGIN      
        1 aacaatagaa agtaaaagta atttttgatt agatattttt ttcatattat cctccatatg
       61 gtatttttat aatgcaaata atataccatt tagatttttt aatcaagtac tttctctatt
      121 aaaaaaaatt taaatatata attattattt acaaaaaaat tatactttat agttgaatta
      181 agaaaacgct tatgatacta taaagttaac aaatttgaat atataacagt agacgtgtta
      241 ctggtaatgc aggcaagatc gaatcaattt ttatagatta tctatagagt actatgggta
      301 taatctattt atttggttcg atttttttgt ttaattggcc ataaaacaaa aagcctatag
      361 ttgaaaatgg aggatttttt atggattata aaaatatagc tcaagaaatt ctactaaatg
      421 taggaggaaa agagaatgta aatgaagtga ctcattgtat gacaagactt agatttaaag
      481 tcaaaagcgc ttctaaagtg aataaggata aattatctaa aactgaagga gtaataactg
      541 ttgttgagag tatgggtcaa atacaagttg taattggaaa taaagtaaaa aaagtatatg
      601 atgaggtcat aaaaattgta cctcaatcaa ataacatagg taaaaagaat gaaagtcaag
      661 aaaagcaagg tataattaat tctatattat cagcagtagc tggtatattt acacctacaa
      721 tacctgctat agcaggggtt ggtatgatta aaggtatttt atcagttctt gctatgtatt
      781 atatgaataa aaatggagta gatataaaag aaacgcaaag ctatattatc ttaaatgcca
      841 tggctgattc tatattctat tttatgccta ttatactagg atatactgcg gctaaagtat
      901 ttaatgcaaa taaaataata tctatggtat taggagctac actttgttat ccaactttta
      961 ctgctttgat ggcaggtgaa gaatctgtta gatttttagg attggcagta acaaaagcta
     1021 cgtatacatc atctgttatt ccaataatta ttgctatatg ggctctttca tacgttgaaa
     1081 aagtattaga aaagtatata cctgaggtaa ttaaaataat aatggttcca actttatcat
     1141 tggttgtaat gttacctgca acattatttt tatttggacc aatcggaatt tatataggaa
     1201 atgtcataaa cttttcttat aagtatattt atgaattaag cccagcattg tgtggagcat
     1261 ttgtaggtgg gttatggtgt gtgttggtta tatttggtgc tcatagagca ttattaccaa
     1321 taggtataag tgatgttgct caaacaggaa gacagaatct attggcgttt gcaggagctg
     1381 caaacttttc acaagcaggg gctgctctag gagtattctt taaaactaaa aatcaaggac
     1441 taaaaacaat ttctatgtct gcaactataa cagctttatt tggtataact gaacctgcaa
     1501 tatacggtgc caatcttaga ttaaaaaaac ctatggtatg tgccgtaata tgtggtgcat
     1561 ttggaggtgc gataatggga atgggtggag cttatggtaa tgcatttgca aaccaaggtg
     1621 ttttaactat acctgtatat gctgaagcag gtgcattagg tttcttaagt tatctaggtg
     1681 gttgtgctat agcatttttt ggttcagcta tttcaactta tctagttgga tttgaagatt
     1741 tggaagagtc ttctaataag gaaaattcat ctataaaagt agaaacgaag gatggtgtta
     1801 ttgatataac ttcacctgta gagggcgaat gtatagaatt gtcagaagta aaggatgatg
     1861 tatttgcttc aaaagctatg ggagaaggta tagctgtgtt acctacaaaa ggagtaataa
     1921 cagcccctac tgattgtgaa gtagcttctt tatttccaac attacatgca ataggattaa
     1981 aattagataa tggagcagaa atgttaatac atgttggaat caatacagta gagttaaatg
     2041 gtaaatactt tacaaaacat gttaatcaag gagatttagt gaaaaaaggt gataaactta
     2101 tatcctttga tatagataaa ataaaaaagg ctggttatga tgtaactacg ccagttatag
     2161 taaacaatac atttgatttt ggacaagtaa tttcttgtaa atctagttat gtttcaacca
     2221 atgataatat aatttcatta gtattagctg gtaattaagt taatttgact agagataagt
     2281 aaattgattt aaaaggaaat aagtaaatca acaattaaat acatctaaaa gtaagtttat
     2341 tgcaatgaat tgttaaagaa aataattaat atggagtaaa gctattgcaa tgaagtaaca
     2401 aagaaaataa ttaatataga gtaagtttat tgtaatgaat tgttaaagaa aataattaat
     2461 atagggtaaa tctatctcaa tgaaataaca aagaaaagag ttaatataga gtgaacttat
     2521 tgcaatgaat caacaaagaa aataatcaat ataaaaagga gaaatattta tgaatacagg
     2581 atttccaaaa gactttttat ggggagcatc atcttctgca tttcaagtgg aaggagcttg
     2641 ggataaagac aataaaggaa aaaccgtagc cgactataat tcttttaaaa agtctcattt
     2701 acaggcagat actaaagtcg catcagactt ttaccataat tatgaagaag atattgaatt
     2761 aatgaaagaa ttagggatga aaacatatcg tttttcaatt tcatgggcaa gaataatacc
     2821 agatggtgaa ggagagataa accagaaagg tttagacttt tataataaga taatagacaa
     2881 actaatagag tgtgatattg agccattcgt aactttatac cattttgacc ttccatttaa
     2941 attagtagag aaatataatg gttgggaaag tagagaaact gtctatgctt ttgaaagatt
     3001 tgctaaaata tgctttaagc attttggaga tagggtaaag tattggcaac ctcataatga
     3061 acagaattta attgttagag tggaggagag aattaatata tatgatgaaa cagattcatg
     3121 gaagatagat aaaatgcgtg ctcagatgga ttataatctt tgcttagcac atgcattagc
     3181 agtcaatgca tgtcatgaga tgataaaaga aagtaaaatt ggagcagcag tatcatcatc
     3241 tgtaacatat cctcttactt ctaagccaga agatgtatat gcagctagaa tgaatgataa
     3301 ttttaaagtg tactatatgc ttgatatgca tcattatgga gaatatccag ggtattatat
     3361 gaagtatctt gaaaaaagaa atatagttcc tcacatggaa gatggggata aagagatact
     3421 taaaaaagct aaaatggatt ttatagctgt aaattattat aggacaaatt gtgcagaagc
     3481 tttacctgaa gattcacaac atccatttgg attaagagaa ggaactgttg attttagtat
     3541 gtatggacta tttaaaatgt caatgaatcc taacttagag gcttctgagt atggagcagc
     3601 aatagaccca tcaggtctta gagttgcctt aaatgaatat tggcaaagat atcacttacc
     3661 tgttataata acagaaaatg gtctaggagc taaagatata ttagaagatg gaaaaattca
     3721 tgatgactat cgtatagact atttaagaag tcatataaat gcatgtaaac tagctattga
     3781 agatggtgta gagatgcttg gatattgtcc atggtcattt acagaccttt taagttcttc
     3841 tcaaggtttt aataagcgtt atggacttgt ttatataaat agaactgacc atgaagtttt
     3901 agatttaaaa agaatcaaga aagatagttt ttactggtat aaagaagtta tagagaacaa
     3961 tggaatagta aaataaacat aaatatatat tgagggtttt agtatgtaca ttacaaaggt
     4021 attaaataat tcgttgctac ttgcaaagga tgataatgat gaagaaataa tattgatggg
     4081 gaaaggcatt ggccataatt ataaggccgg gtctgagtta aataaaaaag atatagaaaa
     4141 gatttttgtt cttcatgatg aaaatataaa aaagagtatt atacaattgg caacagaaat
     4201 agatgcagaa tattttggta tagctcaaat gataattgca tatggaatag aaaaatataa
     4261 cttaaaactt atgaaccata tatatttggc cttaactgac cacattgcct ttgcagtgag
     4321 acgttttaaa gaaggtataa tgattgaaaa tcactatttg ttcgaaatca aggaatttaa
     4381 tccaaaagag tatgatatag gaaaatatgc tattactgtt tttaaggagg ttcttggttt
     4441 agacctacca gaagaagaga ttggtaatat agcaggccat tttataaatg cacaacaaga
     4501 taatccttat agtgatagaa ataagagaag tgctaaaatt gtaaatgcta ttttgcaaat
     4561 agttcattat cacttttcta ttgtatataa taaagagtca ttttattata ggagatttgt
     4621 aatgcatcta aaggcctttt cacaaagatt tctggctaat gaaccatcaa gagaaaaaat
     4681 agattttata tatgagcaag ttcaaaaaaa ttgtaaggaa gagtatgaat gtgtgaaaaa
     4741 ggtaggagct tatatgtata aggaatactc cagagaatta ccaagacaag aggagttata
     4801 tttgatgatt catattcata agattttagg agaattatgc gaaaatagtt agaatatctt
     4861 aatatgctag taatattata aaaaattaaa aattaactac actttaaata tgaataattg
     4921 cattacttaa gggggttgtc ttaaaattga tttttttaat taaatttgaa gctcaactcc
     4981 ttttttaaac ctttatatca ttacaacttt aatgtttata cgcctgtact tcagatgtat
     5041 atctactttt tacatttctc aacatgcaaa cctaccattt tttgcagtat gttaattgaa
     5101 ataaaccatt gagttaaccc taaatttatt ctggacttat aggaatagtt ccaaagatac
     5161 tttaaaatat tttctggaat aattgcttat ttcacatatg ttttattgtg atgttttgat
     5221 ttaataaatt cttcaactct gtatatagta aatgtattat catgtttgtt ttattcaagc
     5281 acacttataa tacgcttgat tttttcacaa tgatgaaata tactaatggg tgttataggt
     5341 ataagaatcg cattacactt gaagaagtaa gtgataaatg agatataaat cctcatatga
     5401 taaactacta tttcattgag gaacatattc taagtgatgt caagatacta caatatgact
     5461 aaattcctag tgataaaaaa gtctacgaat gagtgtagaa aggggaacta agatgaataa
     5521 agttttaatt atagatgatg ataaagatct ttgtattttg ttaaaaaaga gtatttcaat
     5581 tgaacagatt gaagcaaact attgccattc tgggagagat ggactggttg aactttcaaa
     5641 acaagattat cagttagtag ttctagacgt gatgatgcca ggtatggatg gttttgaaac
     5701 tatggaacaa atacgaaaat atagcagtat tcccatctta atgctgacat caaaaagcga
     5761 taattacagc aaagtgcatg gattgcgagc tggtgcagat gactacctaa ccaaaccatt
     5821 tgaaattaca gaatttactg ctcgtgtact gtctttaatc cgacgttaca cccgttttaa
     5881 tccaaaaaat gaggaaccac agcaactttc tttcaaaggt ttgttgattg accttgacca
     5941 gcatacagtg actaccaatg gcgaacctat gttgcttcca cccaaggaat ttggtgtact
     6001 tctttactgt gcaaggcatc agggtaagat tttgacaaaa cagcagattt atgaagccgt
     6061 gtggggtgag ccatatgtat atgatgacag caatatcatg gctattatca gtcgcctaag
     6121 aaaaaaattg aactagaccc atcatctccc atctatttgc aaacaataaa aggaattggg
     6181 tatcaattca acacggaggt atgaaaatgg atgaacaaac gataattctt ttcttactct
     6241 tgctggcgat tggtctactt tttctttcca tatttgcttt ttccaagctt gggcatattt
     6301 acaagcgcct aaaagatata gaggagatac tcgctgatgt acaaattgga gacgaaaatc
     6361 gtaaaatact aattaagcca tgtgatgtta tggctccatt ggtttatcag cttaatgaga
     6421 ttgtctacga ttatgaaaat aagttacttt ctctgaaaaa gtcggataaa gctagcaaac
     6481 aacttatgac aagtttatct catgatgttc gtacaccatt aacaactctg attggttatc
     6541 tgaatgctgt acacagtggt atagttatcg aacaagagcg ggaggaatac cttgaaatcg
     6601 ctaggcgtag agcctacgac ctcaaggatt acattgatgt tctttttgac tggtttcggt
     6661 tgaactctga tgaatttacg ctgtcaatag aatctgtgga aattgcagag ttgtcacgca
     6721 atattttgaa agactggatt cctatattcc atgaaaagaa actgaatttt gaaattgata
     6781 ttccagaaaa tcgactaatg gtaaacctcg acccagatgg atattcccgt gtggtcaata
     6841 atctggttca aaatgttctt gctcacagta aggctcggca aatcaagatt accatgagtg
     6901 aggattccag aatggtatta ctccgtgttg aggatgatgg tgttgggatt gccagagaaa
     6961 atttaccaca tatttttgag cgactgtata agtgtgataa aggacgttca gaaaaaggaa
     7021 gtggtcttgg tctatctatt gtcagtcaaa tggtggagcg aatgggtggt cagatttccg
     7081 tagaaagtga gattggaaag tataccgtat ttacagtgag cttaccatta atataaaatt
     7141 gtttttcccc catgtgacat gtatacacag ggggattttt tgtaagttat aaagttgcaa
     7201 ggttaataca aggttgttgc aaggttaatg taagattgag gtggtatgat aactttaata
     7261 aacaaaggag gcttttatta tttatgaata atttaattat tgaaacaaaa aatctcacta
     7321 agcaatatgg tgctcaaaag agcgtttcta atttgaatat tcacgttcag aaaggtcgca
     7381 tttacggatt gctaggaaga aacggtgcgg gtaagacgac aactatgaaa atgttattgg
     7441 gtctaacaaa accaactact ggagaaatta aaatatttgg tcaggacatt cacaataacg
     7501 agaagaagct tcttcctcga attggatgtt tgattgaatc accagggttt tatcctaatc
     7561 tgacaggaac agaaaatctc aaaatctttg caaggcttag aggaatacca aaaaaaaatg
     7621 cagtagaatc agcactggat gtagtcggtt tgccaaacaa tgacaaaaag ttattttctc
     7681 aatactctct tggtatgaaa cagaggttgg cgattgctct tgccattatg catgacccac
     7741 aactgttgat tttggacgag cctatcaatg gacttgatcc tattggtatt gcagaagtaa
     7801 gaacgtttat tcgtgatctc tgcacagaac gaggtaaaac tattttgatt tccagtcaca
     7861 ttctttctga aattgccttg ttggcagatg acattggcat tattgaccat ggtattttgt
     7921 tggaggaaga aagtcttgcc caattagaag aaaaaaacag taaatacata cattttgctg
     7981 tatctgatgc agcacaggca gcaatgcttc tggaaaccca acttaacctg aaaaatttca
     8041 aagtagcaga taattacaat attatgctat atgatacaga tgtttctgtt gcagaaataa
     8101 atcgtgcttt cattctaaac aacattgagg tatcagaatc tcatttatgc gaagatactc
     8161 ttgaagatta cttcaagaaa gtaacagggg gtgaaggaat tgcttgatat aatttcatgt
     8221 gaattttcaa aactcaagcg gttaaaattt atactgattt ctattttagg tgcttgctta
     8281 tttccaattc ccgcaacaat tttgattgca agggacaact tgccctttga gcaattgttc
     8341 aaattagttg tcaattttgg atattttttg ctattaccaa ttgtattaag tattgttgct
     8401 agccaactat tttttataga aagagataat gatgtattaa aaaatcttgc aacagtgcca
     8461 gtgcaaaaag gaaaactggc tctggcaaaa ttggctgtgt tgctgtttat atcattgttc
     8521 tactccgttg cagggttagg agctactatc attggaggtt tcattgttgg aatagtagag
     8581 ggggttgctg taaaattagg aatgagcatt gcttttggaa ttatgttttt tgttactgta
     8641 cttcctgttg tggtactcat tgtatatttc aatcgcagct atattttttc tattatactt
     8701 tcttttgtat atgctatttt caattttagt atatccttaa atattatcaa ttttgaacca
     8761 aataacccac tattaagcgt attacctgct cctgttataa tgcgttggtg gatggcattt
     8821 tggggagatc ccactggcga atacacggta cttagacagc catatttgct ctcaactcct
     8881 gcttgtgtag gtattttatg tttgataacc attatagcag tcctgctgat ttgtatgata
     8941 tacaaaaagc aggagaatta ggaggtatct atgcttgata taattactac agaatttcaa
     9001 aaaataaaaa gatataatat tctatggatt ggtgttgttg ctgttctgtt ttcagcacta
     9061 ttggcagttt ttcaggttgt aagttcacat ggcagtgacc ctctaacgta tgagagcttt
     9121 gcaaacggag ttatctggaa taatttcagt cttagttttc catttgggat tacacttatt
     9181 ggaggatatc ttattaaccg tgaatataca gaccaaacct taaaaaacat cctaactgtt
     9241 cctatttcat tacgcaaatt gctagtggga aaattaattg cggttggagg aattactgta
     9301 ttgttaagtc ttttcagctt cttatgcaca tttattttgg ggttgttatt ttgcaatatc
     9361 aatatatctg ttatactcgt tgtaaaatcc ttagttcaga ttttgtctgt taatctgtac
     9421 tgttatgttg ctgttttgcc gattattgct tattttggtc gtaaacaaaa tgcatttctg
     9481 acgggtgtag ggattgcatt tgtatacggt ttttgtggtg tgtttgttgc tggaagaaat
     9541 ctgacagact tttatccaat cacagcaggt cttggtctag ttggatacaa caatggtgct
     9601 ggtactgttt atcagccact tatcggtgct atgactttga ttgtaattct aatcttgaca
     9661 actattctac ttgtatttac acctaattat gataaagtta tgaccatatc taagaaaaaa
     9721 gggataaagc ataaaaagta ttctcataaa taacataaat attgtttcta ttatttaagt
     9781 tgctagacag tgaaaagaaa aaatctgtcg tacagtggct atatgctcat gtattcaaga
     9841 agctacagtt ttgatttttc ataactgtag cttctttttg tatttattaa atcacgacta
     9901 tcataatact tgcaataggt atagcgacaa gagagagtac tgtcatatgt gttttatata
     9961 ttacattaag tatattctaa aatatatgtt ttaaataata tatttgaaaa taggaatatt
    10021 taaataaaaa aaataatatt atatatagtt gacaaaaatt ggcaaatgag atactatata
    10081 aatatagaaa tagtaatcat tactattatt aaaaagggag gaattaatta tgaacttaaa
    10141 aggaactaaa acagaaaaga atttaatggc aggatttgca ggagagtcag aagcaagaaa
    10201 taaatataca tactacgctt caaaagcaag aaaagaaggt tataatcaaa tagctgctat
    10261 atttgaagaa actgctaaca atgaaaagga acatgctaaa ttatggttta aacttttaca
    10321 tgatggtatg ccttcaacag aggaaaattt aaaggatgca gcagcaggtg aaaactatga
    10381 atggactgat atgtatgcaa aatttgctaa agaagctaga gaagaaggat ttgacaagat
    10441 agcttattta tttgaagctg ttggaaaaat agagaaagag catgaagaaa gatacttaaa
    10501 gcttttagaa aacttaaatg aaggaaaaat atttaaaaga gatgaagaag ttgtttggca
    10561 atgtcaaaac tgtggtcatg tgtatgtagg tactgaagca ccagaaaaat gcccagtatg
    10621 tgaccatcca aaagcatatt tcaatataaa agctgaaaat tattaaaaat tatcattatt
    10681 aaaaaggaat gttaactatg aaattttcta aacaacgaga actgatttta aatgaaatat
    10741 taaataatcc ggttcatcct actgcggatt acctatatga aaacttaaaa aaagataatc
    10801 caaatttaag cttaggaact gtgtatagaa atttagctca gctaacggaa catggcttta
    10861 taagaaaggt tagtattcca gggtatccag atagatttga tggtcgaata gataatcact
    10921 atcatatcat atgtgaagta tgtggagagg tatacgattt agaatccgaa gttcttaata
    10981 atttacaaga attaatatct gaggagacag acataaaaat aacatcttat aacataagtt
    11041 ttaaagggat ttgtaataat tgtaaaaggt gtagccaagt aggttagttt tcaaattcta
    11101 aaaataatta atgaggtgag tttatgtgta gtgaacaaaa attttttata tgtgagactt
    11161 gtggaaacct tgtaggaatg atacaaagtg gtggtgtacc tattttctgt tgtggaaaac
    11221 ctatgaagga acttgttcct aatacaacag atgcagcagt tgaaaaacat gtacctgtaa
    11281 ttgaagtgga tggaaataat gttactgtta aagtaagtag cacaactcat ccaatgacta
    11341 aagaacatca tatagcatgg gtgtatctta tgactgaaca aggtggtcaa cgcaaatgtc
    11401 tagcagtaga tggagaacct gtagttaaat ttgctttgaa tgatgatgat aaagtgattt
    11461 ctgcctatgc ttactgtaat ttacatggat tatggaaggc tgaactgtaa tatttaaata
    11521 aattgagtca aaaaggaggc tataaatgta ttttcataca aatagcctcc tataaattta
    11581 tataaattca agttttaatt ttgtttaatg tcaatataat ataaggagaa aataatatgt
    11641 caatttataa atgtagtgtt tgtggatata tttatgatga atctaagaat gataaaacat
    11701 gggatgaatt aagtgaagat tgggaatgcc ctgtatgtac aaaaggtcgt agttattttg
    11761 gaaagataag tactgtttac tatgaagaag atgaaaagat agcagaagac atagttgaag
    11821 atgaatctaa attaaatact gaaaaagagg gagatttaaa ttacttaagt acatatttaa
    11881 gaagagatga tgaagttgag aaacatatgg atataattca cgaaatggct gttacaggaa
    11941 aatctataat agaacctatg agaactaaac taccagtaat ttcttgggat gatattttaa
    12001 ttatgggtgc tcaattaaat ccattacctt taaatgagca tgatgaagta aatacaacaa
    12061 caataatagg aaaaaaagca aagaaaccta tgattataga aaatccagtt tatatttccc
    12121 acatgtcatt tggagctttg tctaaagaac taaaaatagc tttagcaaag ggggcagccc
    12181 aaaataagac agctatgtgt agtggtgaag gaggtatatt accagaagag aaggaagctt
    12241 catacaagta tatttttgag tatgtaccaa ataaatatag tgtaactgaa gaaaatctta
    12301 aaaattctga tgctatagag ataaaaattg gtcaaggaac aaaacctggt atgggaggtc
    12361 atcttcctgg cgaaaaagta actgaagaaa tagcaaaagt aagaaataag cctgtaggtc
    12421 aagatgtaat aagtccatct tgttttgagg aaatacaatc caaagaagat ttaaagaaac
    12481 ttgtagatga acttagagaa gtttcagaag ggcgtccaat aggtgttaaa atttcagcag
    12541 gtcatattga aaaagatatg gaatttatag cttatgctaa acctgatttt gtaactattg
    12601 atggtcgtgg tggagctact ggagcaagtc caaagttgct taaagatgca acttcaattc
    12661 caacaatatt tgctctttat cgtgctagaa aatacataga tacacatgga ttagatatag
    12721 atttggttat aacaggtggg cttcgtatat ctactgattt tgctaaggca attgctatgg
    12781 gggctgatgc agttgctatt gcaagttctg ctcttatggc agcggcgtgt caacaatatc
    12841 gtatatgtgg ttctggtaag tgtcctgtag gagtagctac tcaggatgag gaattgagaa
    12901 aaagattaca tatagaaaat agtgctaata gagtggcaaa ctttttaaat gtaagtttag
    12961 aagaacttaa gacttttgct agaatttctg gtcataaaga tatacatgat ttaagtgttg
    13021 atgatttata tactgttaac tctgaaatat caaattatac aaacatacag catgtataga
    13081 ttgaagtttg aaactattag attttagata gctataaaag taaatgataa atatatttta
    13141 taaaagagag tgtcttaaaa tgaacttttt agttcataag taactctttt ttgtatgaag
    13201 ccaaatatat ttatcatttt agcaatgaaa aagaatctta tctctttatt ttgagataga
    13261 ctccttctat ttatgaaaat taaagatatt aaattgtaaa atattctgtg atgtcgtact
    13321 attaaactaa agattctgta ctttaaagta aataaaaatt taaggagtga ttattatgga
    13381 aagattgaag atacaagttt tagtcgacaa taatacttat attgatagat actttgttgg
    13441 agaaccagca gtaagctatt atattgagat tgatggaaat aggattttgt ttgactcagg
    13501 gtattcagat gtattcatat caaacgctga aaaattaaat atagatttag gaaatttaac
    13561 tcatgttgtt ttttctcatg ggcataatga ccatacaaga ggtatccagt ttttagaaaa
    13621 taggtatgat ttatctacag ttgaacttgt ttcacatcct aactgtttta tacctagaaa
    13681 acatggagag aaaagcatag gagctccatt ttctgctgag gaaattaaaa atatttttat
    13741 gtataatcct aaggataagc catttaatct aagcaagaat tgtgtgtttt taggtgaaat
    13801 accttctata aatgattttg aaaaaagagc taaaattggt aaatgtaaaa taggagattt
    13861 gtgggaagat gattatgttt tagatgattc tgctatagtt tgtaaaactg ataaaggtat
    13921 ttttattgtt acaggatgtt ctcatagtgg aatttgtaat atagtagagt atgctaaaaa
    13981 ggtatgtgga gatgatagag ttattggtat actaggtggt tttcatcttt ttgaattgaa
    14041 taataggtta gatagtacaa tacaatattt ggataaagaa gaaataagaa tgttgtaccc
    14101 ttgtcactgt gtttctttta aggtaaaagc gaagatgaat gaaatattga atataaatga
    14161 agttggagta gggctgacca taagtatata gtatttgaaa gtatataaga tttaagttga
    14221 aggttaaaaa gatattttct atagaaaata tcttttttgt tacgtttatt ttaagagaaa
    14281 tcaatatact tttatatgaa atattgtaaa aaaatttatt tttaatgaga atatattttc
    14341 attaataaac aattttatta tgtattaata atgttagaat aaatataaat aaatcaaagt
    14401 tacttacatt acagtatatt ttttcacaaa atatattgat ttattctttt aaagtttgat
    14461 aattccaatg aatgtaataa aagaaggaat tttatactat acgattatta ttataaaata
    14521 ataaagaaaa aattatataa taataaagaa aaaatgtata atatttaaca ttaaaaaaga
    14581 tatttcataa aattaaaaaa caaagtgaat ttgaatacta tttaaaaaat aaaataaata
    14641 tataaaaaat atttaatata aaggtagaaa taagttgaaa atgtaaagtc attggaaaaa
    14701 tatagttgaa tttaatgagc aaaaatgtta ttgttttatc aataaagtgt taaaataaaa
    14761 actttcaata tcattttagg gaggatgatt tcatgcaaaa aagtgtaaag aaagcaaaag
    14821 taacgggctc tatgttagcc atttttggtg ttgcaagcgt tctttttagt tcacatgcag
    14881 gaggaggatt tgctacaggg aatcaagaaa ctcagtacta tgtgcagtat ggatggacag
    14941 caccacttat ggcaatttta gcaatgatta tacttactgc taccatgaga gaagttatca
    15001 taatgtataa taataacaat tgtaggaatt ataaggattt attttgtgag ctttggagac
    15061 catatccaaa acttgaaata atatgggaga tatattacta tctaatggtt ttaatagctg
    15121 taagtgctgt tatagcaggt gcagcagcag tatttcaaag tataggagta aattattttg
    15181 tagctgtatt tattattgga gttgtacttc ttgtctttac tatatttggt gctatgcttg
    15241 tatctaaagc agctacagca atgacaatag ctattctagt atgtactctt actattttta
    15301 ttgttggtat aaaagctaaa gtaccagaaa taactgaaat attatcaaat agaacgagtt
    15361 tcacacctgg ctatatcaag ccaatactta atacttttat ttatgcaggt tttcaatcgg
    15421 ttgttatacc tacacttgca ggatgttcaa gaccacttct taagaactca aaagaagcta
    15481 ctaaggcaat gataatttca tttgttatga atgcaattgc attaggactt gctgtaacta
    15541 tgctaatggg atggtataga gaaattattg ctgctgggca aacaacactc cctactcttt
    15601 atgttgcagg tcaatctgga aatcatacta tatacataat ttataatgtt gcattattcc
    15661 tttgtttaat gtctacagga gtaactacta tatttggtct tgtaaataga tttgaagacc
    15721 ataaagcact ttctttcctt tcttcaagaa tgaaaagaag ggtatttaca gcttgtgcaa
    15781 taatggtagt gtcaatgctt atatcactta caggattaag taatattgtt aaatatggat
    15841 atggatattg tggttatctt ggtctattca ctatagtaat tccattcctt acactaggac
    15901 attataagaa taagaagttt gcaaaggaaa atccagaagc taaatggcca gcggaattaa
    15961 atgaaaatgt aaattagatg tagtagcaaa aaatattgct aaagagaata ctatagataa
    16021 gaatactata gataatatat ctgcatgttt attaagatta tttgcaaaat ctaatattaa
    16081 acaactataa acaagcaata taaaactaag aataaaagat aaaaagaggg tgtctcaaaa
    16141 tggactttta gtttataagg cactcttttt tatatgaagt caaatatatt ttcattattt
    16201 ttacaataaa agagactaat cctactttgg atagcctctt ttttctttaa tataaaatag
    16261 tataacagca aagtatagat tgatacatgt tttctgtagc tttctatata ttacagtgtt
    16321 tgtgtggcat tggtatttta agcaatctat aaattataat tctataattc ttctactaat
    16381 tttagattct atagccgtct ctatttcact taacattcgt tcatttttag ggtcaataaa
    16441 agcacatgta tatccttctt caccagctct accagttcta ccaatacggt ggatgtagtc
    16501 ttctggtgtt tctggtaaat cgtaattata tatatgactt actccagata tatcaagacc
    16561 acgagaagct acatctgtag ctattaagta ttgtatatct aaatttctaa atgatttcat
    16621 tattctttct cgttttgatt gagcaatatc actatgaagt ttttgacaat tatatcctct
    16681 tatagcaaga gcttcttcaa gattgtcaac ccttctctta gtcctacaaa atattattgc
    16741 cataaaagga ttatcttcat ctagtacctt acataaggca tctaattttt ttctatctgt
    16801 agtttctact acttcttgtt ttatattatt aagagtgatt tcttcttttt gaatagaaac
    16861 tacaacagga ttttttgtgt atctataagc taattttttt acttgagaat ttatagttgc
    16921 agaaaagcat agtgtttgct ttttgtttga agtctctttt aaaatagctt ccacttcatt
    16981 tttaaatccc ataagcaaca tttggtctgc ttcatctaaa acaaaagtct ttaacttact
    17041 aaggtctatt gtttttctat ttagatggtc taaaagccta cctggtgtag ctataatcat
    17101 atgaatattt ccttttagtt tctttatttg agaaccaata tcttttcctc catatgctgc
    17161 aagaatactt acatctttag attctttaag tttcatagct tcttctgtta tttgaatagc
    17221 tagttctctt gtaggagaaa gtataagtac ttgtatatca tttatatcta atgatatatt
    17281 ttcaaatatc ggaagtaaaa aagctagtgt ttttcctgtt ccagtttgag cttctgctat
    17341 tacatctttt ccttctttta ttagtttaat actttctttt tgtataggtg taggtgttgt
    17401 tattcctgat ttttttagaa tatttattgt attttcacta atccctaagc ttttgaaatc
    17461 cattgttatc ttacctttca tgaaaaattt tcttttttat taaccaatta ataatatcat
    17521 atttgtaagt atttttagta aattttttaa ttattattta tatagcttaa aaa
//
LOCUS       DBGJFF010000078        17547 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.079, whole genome shotgun sequence.
ACCESSION   DBGJFF010000078 DBGJFF010000000
VERSION     DBGJFF010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17547
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.079"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            453..2540
                     /locus_tag="ACOIAF_002534"
     CDS             453..2540
                     /locus_tag="ACOIAF_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HGZ2669539.1"
                     /translation="MVVDKEIVSILQILLNSTLITTNGLQEESNSSKRQITYRINKIN
                     DMLKVKNVPLICLRADKDIIVRKETKKAIKEILEKNYSKNTYYFSKTERLLYMYLMLF
                     INMEDISINHFINSIKVSRSTVNLDFKDLIPKLEKKNIKVKNNRINGYYIVGNEMEIR
                     RVLIKNIIETLSNEKSSRVFDIFIKEYNLDSFEEAKKIILKLIKKYEITFVEDRLIEF
                     IYIFIFLKARMYSTRIVTQENVDIPNISVMSSMKEYKFAKELLEVYGSEDKIKPHNIM
                     YISAWILGISVGNYEEDTEDKDVISKIVNKMMTKFGYLSGVYYISQEKIFKQLYSHFR
                     PAYYRLLFKLPIYNPICEKVKEEYKLVYRIVSNAMKEFCGLFGEEIQEEELAYLTMHF
                     ATLFSNKKEFDGFRKRIALIVCSNGVGSSAILHAELTNLFPDLHFIPMSESVGLENIS
                     EPVDIVFTTNYNAEGLKTDVPVIKVSPVMTTKEKYKITREVYIQLGDIFLRQPKIDEV
                     MDIIKKYANVTSESILSSELLAYFTQIENFTSKEGEGPMLSEITNETLVKIKVKAKNW
                     EEAIRQSASVLVENNKVTEGYVDAMINSAKASGPYIVITKHVALPHARPEAGSKEIAI
                     GIATLDSPIEFGNNENDPVKYVFCLSAIDNNSHLRAMSELVELLEEDEFFKVLDSAKE
                     AKEIIDFIKTHEL"
     gene            2555..2842
                     /locus_tag="ACOIAF_002535"
     CDS             2555..2842
                     /locus_tag="ACOIAF_002535"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGZ2669540.1"
                     /translation="MYKILIACRAGVGSSLMLKIKTQQIIKENNFPIEVEHGSLDSLN
                     GFNGDAVETLIDVAEELKNKNLKFDIIGIHNIVDRNEIKTALEKFLASKEG"
     gene            2869..4140
                     /locus_tag="ACOIAF_002536"
     CDS             2869..4140
                     /locus_tag="ACOIAF_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HGZ2669541.1"
                     /translation="MEFIISLLSTPAVLLGVVACIGLILQKKSAVEVFTGSSKTLIGF
                     LIFGIGASAMTGALQNFNKLFQHGFGITGVIASPEAATALAQGTYGFAVSCTLILGFI
                     LNLVFARITKFKNIFFTTGHSLFFSCVLVLIMKAHGFDNTITILIGGTILGFMSAALP
                     QFCQPFMRELTGGDEQAIGHFNMIGYGLSGYIGRLFSKHKDDTTETIEFPKWLSIFRD
                     FLMGLSIVMLILFYIATLKAGQAFTQEIAGSTHWLVFPLIEAFTFVAGMSILMSGVRM
                     FLAEITAAFVIISEKYIPGSRPALDVPTVFPYAPNAVIIGFISAYAAGLLAIAIMASF
                     PSIFPVVIIPAAHICFFSGGTAAIFGNTSGGWRGAIAGSFVVGLLLAFLPVILYPVYG
                     TLGIEGATFPNIDYNVVGSILHNILQFIKPI"
     gene            4198..4797
                     /locus_tag="ACOIAF_002537"
     CDS             4198..4797
                     /locus_tag="ACOIAF_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HGZ2669542.1"
                     /translation="MENTLSEFLDNISNELSEFIASIDETSINKACELILEAEKNHGR
                     VHVTGIGKPGHVSGYISSLLSSTGTSAYILHGTEAVHGSSGQVVEGDVVIAISNSGET
                     QELKATLKTLKVNGAKIIGVSGNESSFLKNISDIFLFAGVKQEGDCLNKAPRASILAE
                     TIILQSLSVVLQYAKGLNTQQYLKWHPAGSLGKSIREGI"
     gene            4802..5707
                     /gene="dapA"
                     /locus_tag="ACOIAF_002538"
     CDS             4802..5707
                     /gene="dapA"
                     /locus_tag="ACOIAF_002538"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HGZ2669543.1"
                     /translation="MKYKGIICAMITPFDENQNINSQATCQLIDYLIEKGIYGLFILG
                     TNGECHVLTDDEKVEFAKIVINHTNNRVPVFVGTGGNSTREVVDLSKRMEAIGASALS
                     IITPYFVTPTQQELILHYKTIANSVNLPIIMYNMPGKTGINIEPESVRELSKIKNIVG
                     IKDSSGKLDNMKAYIEATKNENFSVFSGSDSLILDSLKAGSQGAVAATTNFLTEIGIS
                     IYENFMKGDLEEAQKAQSSIEELRRILKLGTIPSVMKKTIVLNGINVGTARLPVTEPT
                     GEVLEEIKRVVENYRTILNKSSENR"
     gene            5813..6445
                     /locus_tag="ACOIAF_002539"
     CDS             5813..6445
                     /locus_tag="ACOIAF_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketohydroxyglutarate aldolase"
                     /protein_id="HGZ2669544.1"
                     /translation="MKKMNITKRMSECGALAIVREENLNRACEIAEGCIKGGITVIEM
                     SYTLNNAGEIIQGLNKKYGETLCVGAGTVLDSETARHAILHGAQFIIAPNYNEGVAKI
                     CNRYQIPYAPGCTSLTEAVDALSLGATFIKAFPISDFYGPKLVKVFKTPIPDMPVLAS
                     GGINIENLHIWLENGVDVCGFGGLLTKGSVEDIAKNAKKIREIIKNIRKI"
     gene            6923..9430
                     /locus_tag="ACOIAF_002540"
     CDS             6923..9430
                     /locus_tag="ACOIAF_002540"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HGZ2669545.1"
                     /translation="MKEDIRLHRMCKKEQATKNEVISEDILKSMSIGVSKHFLDDSFT
                     IIWGNTSFYQLLGYTEEEFLVKFSSLKDYYEQNLYHFESMKNHFLEAYHHGEKSIEYQ
                     ICISANNGDPVWVIMTGILIESQDNEKSMVYILYSKINELKIKQEELSDMEKESTENF
                     KWMMSEYAGNVYISDMDTYELLYLNKHSCNTLQASANELIGRKCYEVIQGRTSPCPFC
                     TNSYLKKDDIYEWEFYNKKLKRTFIIKDRMLNWQGRRVRIELSYDMYSAEYKLAKKDQ
                     ERESILKTIPAGMVRLDARDYLTILWCNDIFLNMIEYTKEQFAEELNNQCGYLFSEDY
                     KRSKKLAQDLKESGDNVVFEAKIYTRSREERIWTVTLCYISGEDSWDGIPSFYSIGID
                     ITKERKQIEKLQHISEKDALTGIYNRAETERQIKKYFEKNLNVMGALFMIDTDNFKQI
                     NDTEGHMIGDIVLTEMASGMKKIMRDSDVVGRIGGDEFTIFMKNISSVKDAEKKAEEL
                     LYMFRHLFQKEKSFLKVTCSIGIAIYPKDGTTFKEIYARADKALYQAKNMGKNNYVIY
                     NQDYFKELEEFDCSSLGTVIDSEKQYAEYPDNLTRYIFRMLYQTDDIDQAINMILEVA
                     GKQFDVSRVYIFENTEDGRYTSNTYEWCNEGISSQMAYLQNSNYQDYEDYEKLFEDEL
                     VFYCRDIHTLSPKLEELFSSQGIHSTLQCAYKEDRVFSGFVGFDECTGLRLWSQEEIS
                     TLSLISQIISMFLQRKKNNKLNQQIQQYQNILDNIDQCICVINKENNFLLYVNKKFKN
                     TYSKFQIGQPYYLDSIDIHKISIIWNKKEAYLCTSTI"
     gene            9772..11934
                     /locus_tag="ACOIAF_002541"
     CDS             9772..11934
                     /locus_tag="ACOIAF_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HGZ2669546.1"
                     /translation="MKKIIYVLFTIELLIVSLLGLNIVKDNEINNILYNDTTSISVMF
                     KDYKKLNKNYSKWIKDIADENNVTISKYVFNNNEKLSIYTTDTSLNNNIKLKSGKFPN
                     TNSNEFISNINTNNSNQVGKFSTMSKDISILIRDFGKISEVGESGILYISTQNEDTIN
                     KILRELNVDRSIFVARLHDRYVNSNLYLNPSIIRDLILIILCFLATIIHYSISVSREA
                     SILRLNGYSKLDIIARVIKNILRIMILSSVTALLIYIIYILKLNIGVRACLYFAMFSI
                     ICILINILISILIVGFNSRSSKYVLSLNGKKSYGFVNIMHFTLKIVFVSFLILSINNC
                     ILNYNMLQTQLGNLSEWDKAKNVYNLTLKDTGEQSLGKEEVIKNAKIKGFYKNLVEEK
                     DAFLIDSTNFEKLEDGSYMYEINSKGKNPEVSQYGKNIKINKNYLKVNPIYSNGKEIF
                     NLIDYGDKTINLLVPKKLQVHENEIKKEFRELFYFEKIEVENMYNKELNRELNKTKKS
                     DLNVNIIYVDNNQSYFTYNSLVMDDNRNLIDDPIAIVDIDNIDDSFYLSYISRCVYFN
                     SKKLDALADISNIIEAQGVEAHIQSLHAVYNEYGLEINKLEKYLNSEIFTIIIIAISN
                     LMITYNIVASYYERNKYKLYIKKIFGYSTTARSMLLIVSLILTNIIPIGIISTRVDLS
                     NNIILFGLLILVIEVIVSIALDKIISNSSFNKIIKGEH"
     gene            11939..12556
                     /locus_tag="ACOIAF_002542"
     CDS             11939..12556
                     /locus_tag="ACOIAF_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2669547.1"
                     /translation="MISLINISKKFKDKSIIDKFNLEISKGEFVAITGNSGSGKTTLL
                     NIMGLLEKPNSGDIIINNIKNPNSKQIRILQRDFYGYLFQNYALIENENVENNLKIAL
                     KYQKNINHNEKINYALESVGLRKYNKKKIYELSGGEQQRVALARVILKECEVIFADEP
                     TGNLDKKNRDIVFDILKSLNKNGKTIVFVTHDLELSEQADRVIKL"
     gene            12745..13089
                     /locus_tag="ACOIAF_002543"
     CDS             12745..13089
                     /locus_tag="ACOIAF_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HGZ2669548.1"
                     /translation="MENLPNCPKCNSEYTYEDGTLLVCPECSHEWALGLGAEEDSNII
                     KDVNGNVLNDGDSVTVIRDLKVKGSSSSIKIGTKVKNIRLIHDSSDGHDIECKIDGFG
                     AMKLKSSVVKKA"
     gene            13659..14525
                     /locus_tag="ACOIAF_002544"
     CDS             13659..14525
                     /locus_tag="ACOIAF_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HGZ2669549.1"
                     /translation="MIDIHKNIYDNKLFEELKIDCKKCFGLCCVALYFSASDGFPIDK
                     ESGKPCINLQPDFKCSVHNSLMKRGFKGCTAYDCFGSGQKVAQVTYKGIDWMQSSELT
                     NQMSEVFLIMRQLHEMLWYLKEASVLNISDTIKSKIDLIIEETEKITNMGPEQIINLD
                     IISHRTKVNLLLSQASESVMGKVKSFIKTSTLKNMKKLSKNIDLIGADLRKINLIGAD
                     LRGRFLIAANLRNTDLSGANLIGADLRDCDIRGANLENSIFLTQLQVNTAKGDSSTKL
                     PASLIRPKYWEK"
     gene            14638..15042
                     /locus_tag="ACOIAF_002545"
     CDS             14638..15042
                     /locus_tag="ACOIAF_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmaF family protein"
                     /protein_id="HGZ2669550.1"
                     /translation="MSYQNYKCERDYVYDDYKYNKCNKCYNDYEEMTHVHEYSESVKL
                     AEECEDRHNHRAAGVTGEAIPINGGTNHVHKINDNVDFLDHFHKICVTTGPAIRIPGT
                     DKHIHLICGETTVNDGHCHKFLFTTQIEAPLV"
     gene            15209..15658
                     /locus_tag="ACOIAF_002546"
     CDS             15209..15658
                     /locus_tag="ACOIAF_002546"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGZ2669551.1"
                     /translation="MKSGIILEMKQINSTEYDEMLQVWESSVRATHDFLTEKDIESLI
                     PLVEIGLKEVENIVCIKDNDIIKGFIGIDKDKIEMLFIEDKYRGNGIGKKLIKYAIDK
                     YNVKYVDVNEQNKKAVGFYIHLGFKVFDRSEIDGQGNPFPILHMKLI"
     gene            complement(15814..16191)
                     /locus_tag="ACOIAF_002547"
     CDS             complement(15814..16191)
                     /locus_tag="ACOIAF_002547"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGZ2669552.1"
                     /translation="MLTPYLIFNGTCEKAFNFYAEAFGGGKTIFARLDSNPNNPIMHA
                     SVTFTKYEGCIMGADTDKPVVISGMAICVVLPSREAIEEISVKLAEGGTLVQEFLPHP
                     PPHQNDGAAEVLDRYGYTWYLST"
     gene            16462..17370
                     /locus_tag="ACOIAF_002548"
     CDS             16462..17370
                     /locus_tag="ACOIAF_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing protein"
                     /protein_id="HGZ2669553.1"
                     /translation="MNIIEIRSDKIYKKIMDAPINKKEDIYRYELMKPFEFKWKCMNV
                     PIVARQKGGYDVIIASEMLGVLSPKDIDEKQKKNINVLSADKIWATCKETIENSINAF
                     IKEGYDLNIKDYKYSILLANPNSPYTILSDGYWGDGGIPGYIFLSLVPNEYTINRLPV
                     LIAHECNHNIRFQFIEWNNNVTLEEMMINEGLAENFATWMFGEEMLGPWVSRTDIETL
                     NTYIKPIIKSALKETGFQNITSYLYGDDIAKMQGYFPVGLPYCAGYACGYYMIKYYLE
                     KTNKSIIEATLLPYSEIIEAVKEFWE"
BASE COUNT         6875 a         1887 c         2955 g         5830 t
ORIGIN      
        1 tttaactttc ttggtttaaa atattaaaat tataaggaac atttattatt aaatacttta
       61 tttatatgaa ttaattgaag aaaaaggata ttaaactaca agttaaaaaa taaatttaat
      121 atattagaat tataaataag gcacattaat tttattttaa attaatgtgc cttttattta
      181 tggtaattta tgcataatta gtatcgtttt gattctttaa ataaattttt cttggtttct
      241 tcacataagg aacttttttt aatttataaa atctacagtt atattatagt atctatttta
      301 gagaagttta atttttgtat caatctaaaa caaaagttat acttaaatga aattgaaaac
      361 gataaaattc tcaaataaaa taagtattgt ggaggtgatg aaagaatcca atgataatat
      421 gaagactaat cttaataaga aagtaggggt taatggtagt ggacaaagaa attgtatcaa
      481 ttctccagat tttattaaat agcacattga ttacaactaa tggattacaa gaggagtcta
      541 attcatcaaa gagacaaatt acttatagaa taaataaaat caatgacatg cttaaagtaa
      601 aaaatgtacc acttatatgt ttaagagctg ataaagatat tatcgttaga aaagaaacaa
      661 aaaaagcaat taaagaaatt ttagaaaaaa actattctaa gaatacttat tattttagta
      721 agacagaaag acttttatat atgtatttaa tgctttttat taacatggag gatatttcaa
      781 ttaatcattt tataaattct ataaaagtaa gtaggtctac tgtaaatctt gatttcaagg
      841 atttgatacc aaagcttgaa aagaagaaca ttaaagttaa gaacaatcgt ataaatggat
      901 attacatagt tggcaatgag atggaaatta ggagagtgct tattaaaaat attatagaaa
      961 ctttgtctaa tgagaaaagt tcaagggtat ttgatatttt tattaaagaa tataatttag
     1021 atagttttga agaggctaaa aaaattatat taaagctaat aaaaaaatat gaaattactt
     1081 ttgttgaaga tagattaata gaatttatat atatatttat ctttctaaag gcaaggatgt
     1141 attcaactag aatagttact caggaaaatg tagatattcc taatatttca gttatgagtt
     1201 caatgaagga gtataaattt gctaaggaat tattagaagt atatgggagt gaagataaaa
     1261 taaagcctca caatataatg tatattagtg cttggatttt aggtatatca gtaggaaatt
     1321 atgaagaaga tacagaagat aaggatgtaa tttcaaaaat tgtaaataaa atgatgacaa
     1381 agtttggata tttaagtgga gtttactata taagccaaga aaaaatattt aaacaattat
     1441 attctcattt tagacctgcc tactatagac ttttatttaa attacctatc tataatccga
     1501 tttgtgaaaa agtaaaagaa gaatataaat tagtttatag aattgtaagt aatgcaatga
     1561 aagagttttg cggattattt ggagaagaaa ttcaggaaga agagttagca tatcttacaa
     1621 tgcactttgc aacacttttt tcaaataaaa aagaatttga tggttttaga aaaagaatag
     1681 cattaatagt ttgttcaaat ggcgttggta gttcagctat tttacatgca gaacttacaa
     1741 acttatttcc tgacttacac tttataccaa tgtcggaatc agttggatta gaaaatataa
     1801 gtgaacctgt agatattgta tttactacta attataacgc agagggttta aaaacagatg
     1861 taccagtaat taaagtaagc cctgttatga ccacaaaaga aaaatataag attacacgtg
     1921 aagtgtatat tcaattaggt gatatttttt taagacaacc taaaatagat gaggttatgg
     1981 atataataaa aaaatatgca aatgtaacct ctgaaagtat tctttcaagt gagctattag
     2041 cttattttac acagatagag aattttacat ctaaggaagg agaggggcct atgctaagtg
     2101 agattactaa tgaaacacta gtaaaaataa aggtaaaagc taagaattgg gaggaagcta
     2161 ttagacagtc agcttctgta ttagttgaaa ataacaaagt aactgaaggg tatgtagatg
     2221 ctatgattaa ctcagcaaaa gcatctggac catacatagt aattacaaaa catgttgcac
     2281 ttccacatgc tagaccagaa gctgggtcaa aagagattgc aataggcatt gctactttag
     2341 atagtcctat tgagtttggg aataacgaaa acgaccctgt aaaatatgta ttttgtctaa
     2401 gtgcaataga caataatagc catttacgtg ctatgtctga gctagttgaa ctattggaag
     2461 aagatgagtt ttttaaggtt ttagatagtg ctaaagaagc taaagaaatt attgatttta
     2521 taaaaacaca cgaattataa aggaggattt aattatgtat aaaattttaa tcgcatgtag
     2581 agcaggagtt ggctcaagtt taatgttaaa aataaagact caacaaatta ttaaggaaaa
     2641 taactttcca attgaagtag agcatggttc attggattca ctaaatggat tcaatggaga
     2701 tgctgtagaa acattaattg atgttgctga agaattaaaa aataaaaatt tgaagtttga
     2761 tataattggg attcacaata ttgttgaccg taatgaaata aagacagcac tagaaaaatt
     2821 ccttgcttca aaagagggat aaatttaaga tgataaggag gaatatttat ggagtttatt
     2881 attagtttgc tgagtacacc tgctgttttg cttggcgtag ttgcatgtat aggattgatt
     2941 cttcaaaaga aatcagctgt tgaagtattt acaggctctt caaaaacact tatcggtttt
     3001 ttgatatttg gtattggtgc ctctgctatg acaggagcac ttcaaaattt caataaactg
     3061 ttccaacatg gctttggaat tactggtgtt atagcttcac cagaagcagc tacagcactt
     3121 gcacaaggta catatggttt tgcagtttca tgtacattaa ttcttggatt catattaaat
     3181 ttagtatttg cacgtattac aaagtttaag aatattttct ttacaacagg tcatagtttg
     3241 ttcttttctt gtgtattagt tttaattatg aaagcacatg gatttgataa tacaatcact
     3301 attttaatag gtggaactat cttagggttt atgtctgctg cactacctca attctgtcaa
     3361 ccatttatgc gtgaattaac aggtggcgac gaacaagcta ttggtcactt taacatgatt
     3421 ggttatggtt tatcaggtta tattgggcgt ttattttcaa aacataaaga tgatacaaca
     3481 gagactatag aatttcctaa gtggttatca atattcagag acttcttaat gggtctatcc
     3541 atagttatgt taatcttatt ttatattgca acattaaaag ctggtcaagc atttactcaa
     3601 gaaattgctg gttcaacaca ttggttggtt tttccgctta tagaggcatt tacatttgtg
     3661 gcaggtatgt caatattgat gtcaggtgtt cgtatgttct tagctgaaat aactgcagca
     3721 tttgtaatca tttcagaaaa gtatattcca ggttcacgtc ctgcgctaga tgttccaacc
     3781 gtatttccat atgcaccaaa tgcagttatt attgggttta tatcagcata tgctgctggt
     3841 ctattagcaa ttgctattat ggcatcattt cctagtatat ttcctgtagt aattattcca
     3901 gctgctcaca tatgtttctt ctcaggaggt acagctgcaa tatttggcaa tacttcagga
     3961 ggatggagag gggctattgc tgggtcattt gttgtggggt tattacttgc atttttgcca
     4021 gttatactat atccagttta tgggactctt ggtatagaag gagcaacttt ccctaatatt
     4081 gattacaacg tagttggttc gatattacat aatattttac aatttataaa acctatttaa
     4141 actaaattat ataaaagtat agtcatagtt actatgattt caaggaggat acatcttatg
     4201 gaaaatacat tatctgaatt tttagataat atatctaatg aattgtcaga atttattgcc
     4261 tcaatagatg agacatctat aaataaagca tgcgaattaa ttcttgaagc tgaaaaaaat
     4321 cacggacgtg tacatgtgac tggtattggc aaaccaggac atgtatcggg atatatttca
     4381 tcattgcttt catctactgg aacgtcagca tatatattgc atggtacaga agcagtacat
     4441 gggtcaagtg gtcaagtagt tgaaggagat gttgttattg caatatcaaa tagtggtgaa
     4501 actcaagagt taaaagcaac tcttaagact ctaaaagtaa atggagcaaa gataattggt
     4561 gtaagtggta atgagagttc atttttaaaa aatatttctg atatattcct gtttgcaggt
     4621 gttaagcagg aaggagattg tctcaataaa gctcctcgtg cttcaatttt agctgaaaca
     4681 ataatattac aatcattaag tgttgtatta caatatgcta aaggtcttaa tacacagcag
     4741 tatttgaagt ggcatccagc aggaagtctt ggaaagtcta ttagagaggg gatataattt
     4801 tatgaaatat aaagggatta tatgtgcaat gataacacca tttgatgaaa atcaaaatat
     4861 taattcacaa gcaacatgtc aattaattga ttatttgatt gaaaagggaa tttatggatt
     4921 atttattttg ggcactaatg gtgaatgtca tgtattaaca gatgatgaaa aagttgaatt
     4981 tgcaaagatt gttattaatc atacaaataa tcgtgtacct gtttttgttg gaacaggggg
     5041 taattcaact cgtgaagttg tagatttatc taagagaatg gaagcgattg gagcaagtgc
     5101 attatctatt attacaccat actttgttac accaacacaa caggaattaa tcttacatta
     5161 caaaactatt gctaattcag ttaatttacc tattataatg tataatatgc caggtaagac
     5221 tggaattaat attgaaccag agtcagtacg tgaattatca aagataaaga atattgttgg
     5281 aattaaggat agtagtggta aacttgacaa catgaaagct tatattgaag caacgaagaa
     5341 tgagaatttt agtgtgtttt caggctcaga ttcacttatt ctggatagtc taaaagctgg
     5401 tagtcaagga gctgttgcag caacaacaaa cttcctcaca gagattggta tctctatcta
     5461 tgaaaacttt atgaaagggg atttggaaga agctcaaaaa gctcaaagta gtatagaaga
     5521 acttcgacgt atactgaaac ttggaacaat tccatctgtt atgaagaaaa ctattgtctt
     5581 gaatggaatt aatgttggaa ctgcacgact tcctgtaact gaaccaactg gggaagtact
     5641 tgaagaaatt aaacgagtag ttgaaaatta taggacaata cttaataaaa gtagtgagaa
     5701 tagataatat aaatagggaa attacactgg tatataatta atatatatat tataaaacat
     5761 gactaaaatt aatgaataag taaagtggtt tgatgagtat agaggtgata atatgaaaaa
     5821 gatgaatata accaaaagaa tgtcagaatg tggggctctt gcaattgtaa gagaagaaaa
     5881 tcttaaccgt gcatgtgaaa ttgctgaagg atgtataaaa ggtggtataa cagttataga
     5941 aatgagttat accctaaata atgctggaga aataattcaa gggcttaata aaaagtatgg
     6001 tgaaacactt tgtgtgggag caggaacagt tttggatagt gaaacagctc gtcatgctat
     6061 attacatgga gctcaattta ttattgcacc aaattataat gaaggtgtag cgaagatttg
     6121 caatagatat cagattcctt atgctccagg atgtacatca ttgactgagg cagtagacgc
     6181 actaagttta ggagcaactt ttataaaagc tttcccaata tcagatttct atggacctaa
     6241 actagtaaag gtatttaaga caccaatacc tgatatgcca gttttagcaa gtggagggat
     6301 taatattgaa aacctacata tatggttaga aaatggagtt gatgtatgtg gatttggtgg
     6361 tttattaact aaaggttctg ttgaagatat tgctaaaaat gctaaaaaga ttcgtgaaat
     6421 cattaaaaat ataagaaaaa tttaacatag ggctgtatca tagtatctta taattactat
     6481 gatacagcct ttttaatatt attcaaattt aaaacagttt acatttctat tatattacat
     6541 ataatttcag taattttatc ttaatttttc tatctggaaa aaaatgattt aaaacttaag
     6601 ttatgatata atttcattaa aagaaggcga tttttagtaa atatgagaaa ataatgtgtt
     6661 tttaggggaa aagttaaata cagatttaag tgaacaagaa tcgataatta agatattaaa
     6721 tggtgaatag ggcatcaaaa aaattactaa attaaaagtt ttagagggag aatcttagca
     6781 agtgtgtgtg aaaattcaat ttcaaacaaa aaataattgt aaatgaaatc aatttataca
     6841 cgttgaagat tcacttttat cattatatta aaaagcagag tgaattaaaa gtgagtctaa
     6901 agataggagg ataattaaaa caatgaaaga agacattagg ttacatcgaa tgtgtaaaaa
     6961 agaacaagca acaaagaatg aagttatatc agaggatatt ctaaaatcta tgagtattgg
     7021 ggtcagtaaa cattttttag atgattcatt tacaataata tggggaaata catcattcta
     7081 tcaattatta ggatatacag aagaagaatt tttagttaaa ttttctagtt taaaagatta
     7141 ttatgaacag aatctttatc attttgaatc tatgaaaaat cattttttag aggcttatca
     7201 tcatggcgaa aagagtatag aatatcaaat ttgcatttca gcaaataatg gagaccctgt
     7261 ttgggtaata atgactggta tattaattga atcacaagat aatgagaaat caatggtcta
     7321 tatattgtat tcaaaaatta atgagttaaa aataaagcaa gaagaactat ctgatatgga
     7381 aaaagagagt acagagaatt ttaaatggat gatgtcagag tatgctggga atgtttatat
     7441 tagcgatatg gatacatatg aattactata cttaaataag cactcatgca atactttaca
     7501 ggcatcagca aatgaactta ttggaagaaa atgttatgag gtaattcaag gtagaacttc
     7561 gccatgtccg ttttgtacaa attcgtattt aaaaaaagat gacatatatg aatgggaatt
     7621 ttataataaa aaactgaaac gaacatttat aattaaagac cgtatgttga attggcaagg
     7681 gcgaagagtt cgtattgaat tatcttatga tatgtatagt gctgaatata aattagcaaa
     7741 aaaagatcag gaaagagaat caattttgaa aactattcca gcaggaatgg ttagattaga
     7801 tgcaagagat tatcttacta tactttggtg taatgatata tttttgaata tgattgaata
     7861 tacaaaggaa cagtttgcag aggaacttaa taatcaatgt ggatatttat tttctgaaga
     7921 ttataaacgt agtaagaaac tggcacaaga tttgaaggag tctggggata atgttgtatt
     7981 tgaagcaaaa atatatacac gttcaaggga agaacgtata tggactgtta cattgtgtta
     8041 tatcagtggg gaagacagtt gggatggaat accatctttt tatagtatag gtattgacat
     8101 aacaaaggaa agaaaacaga ttgagaaatt acagcatata tcagagaaag atgcattaac
     8161 aggaatttat aatcgtgctg agactgaaag gcagattaag aaatattttg aaaaaaattt
     8221 aaatgttatg ggtgcactgt ttatgattga tacagataat tttaaacaga ttaatgatac
     8281 agaagggcat atgattggag atatagtact tactgagatg gcatcaggta tgaaaaaaat
     8341 aatgcgtgac agtgatgttg ttggaagaat aggtggagat gagtttacaa tttttatgaa
     8401 gaatatttcc tcagttaaag atgcagaaaa gaaagcagaa gaactattgt atatgttccg
     8461 tcatctattt cagaaagaaa aaagttttct aaaagtaact tgtagcatag gaatagctat
     8521 atatccaaag gatggtacta cattcaagga aatatatgca agagctgata aagccctgta
     8581 tcaagcaaaa aatatgggaa aaaataatta tgtaatctac aaccaagatt attttaaaga
     8641 attagaagaa tttgattgtt cttctcttgg tacagttatt gattcagaaa aacaatacgc
     8701 tgaatatcct gataatctta cacgttatat attccgtatg ctttatcaga ctgatgatat
     8761 agaccaggcg attaatatga tacttgaggt tgctgggaaa cagtttgatg taagtcgtgt
     8821 ttatattttt gaaaatacag aagatggtag atatacttct aatacttatg aatggtgtaa
     8881 tgaaggaatc tcttctcaaa tggcttattt gcaaaacagc aattatcaag actatgagga
     8941 ttatgaaaag ctttttgagg atgaattagt tttttattgt cgagatatcc atacattaag
     9001 tcctaaacta gaagaattgt tttctagtca aggtatccac tctacacttc aatgtgcata
     9061 taaagaagat agagttttca gtggatttgt tggatttgat gaatgcacag gactacgtct
     9121 atggtcacaa gaggaaataa gtacattatc attgatttct caaattatat caatgttttt
     9181 acaacgtaaa aaaaataata aacttaatca acagattcaa caataccaga atattttaga
     9241 taatattgac cagtgtattt gtgtgattaa taaagaaaat aattttttat tatatgtaaa
     9301 taaaaaattt aaaaatacat attctaaatt ccaaattgga caaccttatt atcttgattc
     9361 tatagatatt cataagatat ccattatatg gaataaaaaa gaagcatacc tatgtactag
     9421 cacgatttaa tggtaaagta aagtttatat tggacttaaa ttaaatttcg ttttaaataa
     9481 gatattattt tagttgctta ctgaaaagac tagaatctat attaatgaat tatactaatt
     9541 ttataccact attaaaatat tgtttgctaa aaataaaaaa tagccttaaa acagttgatt
     9601 ataaaaaatt aactgcttta aggctatttt tatctttgtg acgaaataga cacttgccat
     9661 ttagaatgtg acaatatagt agttgtttag aaaaggcaca aagcattata ctttaactaa
     9721 agtaaaatgt attttgcttt tagaataaaa acaattagga gggagctata attgaaaaag
     9781 ataatatatg tattatttac aattgaacta ttaatagtat ctttattagg tctaaatata
     9841 gttaaagata atgaaattaa taatatttta tataacgata caacaagtat atctgtaatg
     9901 tttaaagatt ataaaaagtt aaataaaaat tatagtaagt ggattaaaga tattgcagat
     9961 gaaaataatg tgactatatc aaagtatgtt tttaataata atgaaaaatt atctatatat
    10021 acgactgata caagtttgaa taacaatata aagttaaaat caggaaaatt tcctaatact
    10081 aattcaaatg aatttattag taatataaac actaataata gtaatcaagt aggtaaattc
    10141 tcaactatga gtaaagacat atcaatttta attagggatt ttggtaaaat aagtgaagtt
    10201 ggagaatcag gaatcttgta tatttcaact caaaatgaag atacaataaa taaaatatta
    10261 agagaactaa atgtagacag aagtattttt gtagcaagat tacatgatag atatgttaac
    10321 tcaaatttat acttaaatcc ttcaataatt agagatttaa tactaattat cctttgtttc
    10381 ttggctacaa taatacatta ttcaattagt gtgagtagag aggcttctat tttaagatta
    10441 aatggatact ctaagctaga tataattgct agagttataa aaaatatact tagaattatg
    10501 attttatcat ctgtcacagc attattaata tatataatct atattttaaa attaaatata
    10561 ggtgttagag catgcttata ctttgctatg ttttcgatta tatgtatttt gataaatatt
    10621 ttgatatcta tattaatagt tggttttaat agtagaagtt ctaaatatgt gctatctcta
    10681 aatggaaaaa aatcttatgg ttttgtaaat ataatgcatt tcacattgaa aattgtattt
    10741 gtatcatttc ttatattatc cataaataac tgtattttaa attataatat gctacaaact
    10801 cagttaggaa atctatctga gtgggataaa gcaaaaaatg tttataatct tactcttaaa
    10861 gatacaggcg aacaatcttt aggaaaggaa gaagtaatta aaaatgccaa gattaaaggt
    10921 ttctataaaa atttggtaga agaaaaagat gctttcttaa tagattcaac taattttgaa
    10981 aaacttgaag atggaagtta catgtatgaa ataaattcaa agggtaaaaa cccagaagta
    11041 agtcaatatg gtaaaaatat aaaaataaat aaaaactatc ttaaagttaa ccctatttac
    11101 tcaaatggca aagaaatatt taatctaatc gattatggcg ataagactat aaatttatta
    11161 gttcctaaaa agttacaagt acatgaaaat gagattaaaa aagagtttag agaattattt
    11221 tattttgaaa aaatagaagt agaaaatatg tacaataaag agttaaatag agagttaaat
    11281 aaaacaaaga agtcagattt aaatgttaat ataatatatg tagataataa tcaaagctat
    11341 tttacttata actcccttgt tatggatgac aataggaatc tcattgatga cccaatagca
    11401 atagttgaca tagataatat tgatgattct ttttatcttt cttatatatc tagatgtgta
    11461 tattttaatt ctaagaaatt agatgccctt gcagatataa gtaacattat tgaagctcaa
    11521 ggtgtagaag cgcatatcca aagtttacat gctgtttata atgagtatgg tttagaaata
    11581 aataaactag aaaaatattt gaatagtgaa atttttacta taataataat agcaatatca
    11641 aacttaatga ttacctacaa tatagtagct agctactatg aaagaaataa atataagtta
    11701 tatattaaaa aaatatttgg ttattcaact acagctagaa gtatgttgtt aatagtttca
    11761 ttaatattaa caaacattat tccaataggc attatatcaa caagagtaga tttaagtaat
    11821 aatattatct tatttggact tctaatatta gttatagaag taattgtatc tattgcatta
    11881 gacaagatta tctcaaatag ttcatttaat aaaattataa aaggagaaca ttaaaaagat
    11941 gatttcatta ataaatatat ctaaaaaatt taaagataaa tctataatag acaaatttaa
    12001 cctggaaatt tctaagggcg agtttgtagc gataacagga aatagtggtt caggtaagac
    12061 aactttactt aatataatgg gattattaga aaaacctaat tctggtgata ttataataaa
    12121 taatataaaa aatccaaata gtaaacagat aagaatttta caaagggatt tttatggcta
    12181 tctatttcaa aattatgctt taattgagaa tgaaaatgtt gaaaataact tgaaaattgc
    12241 tttaaagtat caaaaaaata tcaaccataa tgaaaaaata aattacgcac ttgagtctgt
    12301 aggtcttaga aaatacaata aaaaaaagat atacgaattg agtggaggag aacaacaaag
    12361 ggtagcatta gcaagagtaa tattaaaaga atgtgaagta atatttgcag atgaacctac
    12421 aggtaattta gataaaaaaa atagagacat agtatttgat atacttaaaa gtctaaataa
    12481 gaatggtaaa actatcgtat ttgtaactca tgatttagag ttatcggagc aggcagatag
    12541 agttataaaa ttgtaataat atcaactata ataaatataa gaaagagatt acagctaaat
    12601 caagtttaat aataatacga atagaattta aacattaagt aaattagtag tttgtaaata
    12661 taagtataat atgatatcat aaaggctcaa aagaaatttt atataataag aaatctaaaa
    12721 ataacaaatg gaggacatat aaagatggaa aatttaccaa attgcccgaa atgtaattca
    12781 gaatatactt atgaagatgg aactctttta gtttgtccag aatgctctca tgagtgggca
    12841 ttaggattgg gagctgaaga agattcaaat attatcaaag atgtaaatgg aaatgtatta
    12901 aatgatggag attctgttac agtaattaga gaccttaaag taaaaggaag ttcatcatct
    12961 ataaagattg gaacaaaagt gaaaaatatc cgtttaatcc atgactcatc agatggacat
    13021 gatatagaat gtaagataga tggttttgga gctatgaagc taaagtcttc tgttgttaaa
    13081 aaagcgtaat attataaata agctgtagtg tttattgttt ttcagtacag tataaaacca
    13141 taagaatcta ttaagtaata aaagtttgtt tatttatctt tatattttct tatggtttta
    13201 ttaaatataa attttattga acaccaaaat attctctcct ctacaaataa gccaatctta
    13261 tttaaactaa tatactttgt actatttata ctgaaattgt aaatttaaat gctatgttat
    13321 ttaggtttgt atagttaatt tatatatttc tgattttata aattgtattt attattatat
    13381 cttgattcta aaatcattaa aaatttcaac ttctataaag atttacaatt tatcaaatac
    13441 attatgagta tataaacaat ttattattac agtgctacat ttaaaataag tcttagtata
    13501 aaggtattgt tacaagtata aagaaactta tgtatgctat tggaataaat aataccttaa
    13561 attataatta agaataagaa ctgttaaata agggacttga ttatcattta gttatagata
    13621 atataatatt aaaaataaag tttaagagga gaaaaaatat gatagatata cataaaaata
    13681 tatatgataa taagttattt gaggagctaa agattgactg taagaagtgc tttggacttt
    13741 gctgtgtagc tctttatttt tctgcttcag atggatttcc tatcgataag gaatcaggaa
    13801 aaccctgtat taatttacaa ccagatttta aatgttctgt acataatagt cttatgaagc
    13861 gaggttttaa agggtgtact gcatatgatt gttttggttc aggtcaaaaa gtagcacaag
    13921 taacatataa ggggatagac tggatgcaat catcagaact aacaaatcaa atgtctgaag
    13981 tgtttttaat tatgagacaa ttacatgaaa tgttatggta tcttaaagaa gcatctgtat
    14041 taaatatatc agatacaatc aaatcaaaaa ttgatttaat aattgaagaa actgaaaaga
    14101 taactaatat gggcccagaa cagataatta atttggatat catatcacat agaacaaagg
    14161 ttaatttatt attatcacaa gcgagtgagt ctgtgatggg aaaggttaaa tcatttatca
    14221 agacttctac tcttaaaaac atgaaaaaat taagtaaaaa tatagattta ataggagcag
    14281 accttagaaa aataaatcta ataggagcag atttaagagg tagatttcta atagcagcaa
    14341 atttaagaaa tacagatttg agtggggcaa atttaatagg agcagactta agagattgtg
    14401 atattagagg tgctaatctt gaaaatagta tatttttaac acaattacaa gtaaatactg
    14461 caaaaggaga ttctagtacg aagttaccag cttcactaat acgccctaaa tactgggaaa
    14521 aataattaaa ttaatacata attttacaaa atatgaactt ttttgacttt aattcatatt
    14581 atacattaga gattctttac aagaatttct taatataata tagggagaga tataaaaatg
    14641 agttatcaaa attataaatg tgaaagagat tatgtgtatg atgattataa atacaataag
    14701 tgcaacaagt gctataatga ttatgaggaa atgacacatg ttcatgaata ttcagaaagt
    14761 gttaagttag cagaagaatg tgaggataga cataaccacc gtgcagcagg ggttactggt
    14821 gaagctatac caataaatgg aggtacaaat catgttcata aaataaatga taatgtagat
    14881 ttccttgacc atttccataa gatatgtgtt acaacagggc cagcaattag aatacctgga
    14941 actgataaac acattcactt aatatgtgga gaaactactg ttaatgatgg tcattgtcat
    15001 aaattccttt ttactactca aatagaagca cctttagtat aagctgatgt gaattattgt
    15061 agtgaatttg ctagattata gtataataat atataataga taatatttaa acttaatgag
    15121 gactatgata tttaaccttc gtagtccttt ctaagttttg tataaaaaag tgttatgcaa
    15181 ttaaaagtga tatagggagg aaatttttat gaaatctgga ataattcttg aaatgaaaca
    15241 aataaattca acagaatatg acgaaatgtt gcaagtctgg gaatcatcag taagagcaac
    15301 gcatgatttt ttaacagaaa aagatattga atctttgata ccattagttg aaattggact
    15361 taaagaagta gaaaatatag tttgtataaa agataatgat ataataaagg gctttatagg
    15421 aatagataaa gataaaattg agatgctctt tatagaagat aaatatagag gaaatggtat
    15481 tggtaaaaaa ttaataaaat atgctataga taaatacaat gtaaaatatg ttgatgtaaa
    15541 tgagcagaat aaaaaagctg taggatttta tattcactta ggatttaaag tgtttgatag
    15601 gtctgaaata gatggacaag gtaacccatt tccaatacta catatgaaat taatttaaag
    15661 ttatacttct cacttattcg caaaaatatt tacttaaatt tttatacaaa taaaagtcgt
    15721 gacagtttta ggacacgact tttttactac actattaaat tgaaatttat ctgttctata
    15781 ttatgattat tagatgtagt ttttttattt taactatgta cttaaatacc aagtataccc
    15841 atacctatca agtacttcag cagcgccatc attttgatgt ggtggtgggt gtggtaaaaa
    15901 ttcttgtaca agtgtaccac cttcggcaag ttttacagat atttcttcta tcgcttctcg
    15961 agatggtaga acaacacaaa tcgccatgcc agaaattaca acaggcttgt ctgtatccgc
    16021 acccattata caaccttcgt attttgtgaa agtaacactt gcgtgcataa taggattgtt
    16081 tggattgctg tctaatcgcg caaatatagt ttttcctcct ccgaaagcct cagcataaaa
    16141 attaaatgct ttttcacaag taccattaaa tattaaatat ggagttagca tgaccaatat
    16201 atcctccttg atatagaata atttatagtt gcacctgata atctaaatta tgatttagta
    16261 aattgtatca tagggaaaac ttgaagtata taatttttta ttattatatt atacaaattt
    16321 attaatttaa aaaaatgtta tatgtacgga aggtttacag gataaaatat aataaaaaga
    16381 ttaaggtgac attataaaaa atgtatattg acattgacgc tacgtaattg tatatacttt
    16441 gtcttaaagg agatggtaaa tatgaatatt atagaaattc gctcagataa aatatacaag
    16501 aagataatgg atgcaccaat aaacaaaaaa gaagatatat acagatatga attgatgaag
    16561 ccttttgaat ttaagtggaa gtgtatgaat gttccaatag ttgctagaca gaaaggtgga
    16621 tatgatgtaa ttatagcaag tgaaatgtta ggggttttat cgcctaagga tattgatgaa
    16681 aagcaaaaaa agaatataaa tgtgttatct gctgataaaa tttgggccac ttgtaaagaa
    16741 accatagaaa actctataaa tgcttttata aaagaagggt atgatttaaa cattaaggac
    16801 tataaatatt caatattatt ggcgaatcca aatagtcctt atacaatatt aagtgatgga
    16861 tactggggtg atggtgggat tcctggatat atatttctat cattggttcc taatgaatat
    16921 actatcaata gattaccagt attaatagca catgaatgta atcacaatat tagatttcag
    16981 tttatagagt ggaataataa tgtaacatta gaagaaatga tgataaatga aggtcttgca
    17041 gaaaattttg caacatggat gtttggagag gaaatgttag gaccttgggt cagtagaaca
    17101 gatatcgaaa cattaaatac ttatataaag ccaataataa aaagtgcttt aaaagaaact
    17161 ggatttcaaa atataacatc ttatctttat ggtgatgata tagctaaaat gcaaggatat
    17221 tttccagtag ggttgcctta ttgtgcagga tatgcttgtg gatattatat gattaagtat
    17281 tatttagaaa agacaaataa atcaataatc gaagcgactt tattgcctta tagtgagata
    17341 atcgaagcag taaaagagtt ttgggaataa attttataaa ttgcataatt tgaaataaat
    17401 tatgcaatta ttttattttt aagaaaaaat aaattgacaa taatttataa gaaatataca
    17461 ataatatata taaagaaata tttcaatatt taaaagtttt aagatttcat atctagagta
    17521 cctaacatta catgttagtt tcttgtt
//
LOCUS       DBGJFF010000079        17427 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.080, whole genome shotgun sequence.
ACCESSION   DBGJFF010000079 DBGJFF010000000
VERSION     DBGJFF010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17427
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.080"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            133..1659
                     /locus_tag="ACOIAF_002549"
     CDS             133..1659
                     /locus_tag="ACOIAF_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HGZ2669554.1"
                     /translation="MNESYWFLNSSPKEYNKLGENIKTDCLIVGGGITGLTTAYLLAK
                     EGKKVVLVEADKIGYGTSGRNTGKVTCQHDIFYSKIEKKYGLDKAKSYYNANNEALNL
                     VEQIIEENNIKCDFKRETSFVFTEKEDTIKNIKDEYRTCKKIGINCEYHETIENIPLD
                     IKGAISFTNQGQFNPKKYIDGLAKAAVNLGLKIYENTPVVDLEKGKICRVKTREDNII
                     EAENVIISSHSPWYDGLNFYFAKEYAERAYLMVAVLENKLADGMFISIDDPSITFRQY
                     NDGSENLLIFGGGDHKVGQGGTEKEIFDDLEHYGKEVFKVKDFKGKWSAQDNMSFDNV
                     PYIGYINKREDNIYVATGFSKWGITNGTAAGIIIKDLIINNNSDYKDTFNPSRLGSYF
                     SKDFIKENANVAINYVSGKLKIGSGDMPKNNGEGKIVNIDGKRYGVYKDDNGDFYIVD
                     TTCTHLGCELNFNSEEKTWDCPCHGSRFDYKGNILEGPALKPLKLYGHGDNDVNPKLL
                     "
     gene            1687..2244
                     /locus_tag="ACOIAF_002550"
     CDS             1687..2244
                     /locus_tag="ACOIAF_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669555.1"
                     /translation="MNKLLKPITILLYIVYILFAVFFVANKEYGSLGLVAISLVGTFI
                     LSFINKQTERLLSKELYIMLALFIMFSSLLGTCFNFYDINYYDDFLHLWSGIISCIVA
                     FSIIKYFYTQREISKMSKVFLVIFLFMFSMGVASLWEIGEFLMDTFVGTTTQAGGLED
                     TMIDMVDALIGTIITIPFIVKKSNI"
     gene            2766..3809
                     /locus_tag="ACOIAF_002551"
     CDS             2766..3809
                     /locus_tag="ACOIAF_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669556.1"
                     /translation="MLKKWFGIVKKRQKSESVKEEGEVILKNEKILSEEKLIDEEGVV
                     VNIDNEILTKVEVVNDDNEIEEKIIEEDLLISENTIKLDDKEAIINDKNIELCKKEVQ
                     VESEKIGLNKFEGLDQNENYNLEKNVIEEKKVSECLTEEDLEYIKEIKIKRGKSIKAI
                     NLYTKEEWVFDTHIQCSKKLKVPLGYIRENLKYGYMDYFGDAINYLSEVLNIDEYCKS
                     EWSYLDNSKSPSEIFNILNNKIFSIRLSNEKRNEILTNDKIEALKMNYRFECIDEEYD
                     EYFKKYKSIIKRGGKKKVELVNKKGDILEIFKSLEECAIYLQKEKNEVIQMLKYGDTK
                     VGRNFIRYSLRSI"
     gene            3920..5062
                     /locus_tag="ACOIAF_002552"
     CDS             3920..5062
                     /locus_tag="ACOIAF_002552"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HGZ2669557.1"
                     /translation="MSEEKNPKENTLDEPKNSDNVNSKKDDVSDEKNTDNKVKKREIK
                     LNNKTLKAIVAIVAVGAICLGGGYVFGKEAGRKLPATSKHYNSSEVVATIGDKKITGE
                     MLRKRMEPLFYLNAKTTMTDDEIRSYESNMIETMITSDILSSEAAKEKISITNDQLDA
                     QYNNLMSSIESAMGMTKEEFLKQFDLTEKYIKDDMKKELVASKYLNEKSKVTDKEAEN
                     YYNKNKSNYLQVRASHILIKTVDDKGKQVSSSKKAELKKEAEEILKKAQAGEDFATLA
                     KKYSEDSSAESGGDLGFFGKGQMVESFEKAAFALKKGEVSNKLVESDYGYHIIKKTDE
                     KYQPFEEVKSDLVSSLTSEKQNLLIQNLKEKYNVKITDKYQMKAKE"
     gene            5516..6907
                     /locus_tag="ACOIAF_002553"
     CDS             5516..6907
                     /locus_tag="ACOIAF_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HGZ2669558.1"
                     /translation="MSSTESVSKKSGHPKSFWLICMTILWERFSYYGLTALLVLFFTA
                     SVAQGGLGLSTQEATSMFGLFTGLIYLTPLVGGWLADKVLGQQKCISIGALLIAIGDF
                     VLFASSTSIAMVWVGLSIIILGNGFFKSSCSNIVGDIYPKTDVSRKDAAYSLFYMAVN
                     VGAFIAPIICGLLSDNVFAVRGASGDIVSYGYKMAFLICGIGMMLGMIIFTLLAPKLL
                     NEVGKYPVTKGNKGEKIEYGPLTKLEKNRIIAMVILFLFVVLFWTAYYQTQSSFMIYV
                     RDNVNRTLFGFEMPVAWLTSLNAILCVALCPVLATLWVKLSHTKRGDFSIPVKMGLGM
                     IIMGVGFIVMVFATLANGPDGMVKASILFIVLTYIFTTVGELFLSPIGLAMFNKLAPA
                     KYATLSMGAWYLTSFFASLLSGKIAGFTATLGFLQIFGGIATVLIIFGLILLGIRNVL
                     TRLMAMDLLTKEK"
     gene            7160..8953
                     /locus_tag="ACOIAF_002554"
     CDS             7160..8953
                     /locus_tag="ACOIAF_002554"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HGZ2669559.1"
                     /translation="MNIKDRLSGLRKFMEEKNIDAYMIPSSDNHQSEYVGDYFKSREF
                     ISGFNGSAGTVIVTKDEAGLWTDGRYFIQAESQLEGSTIKLFKMGQEGCPTTDEYLYK
                     NIPEGGTLGFDGRVISAREGATLAEKLSKKGIKIEYQYDLIDSIWPDRPALSDSKAFL
                     LDVKYCGESFSSKLARLREKMSEKGTSTHVITTLDDIAWLFNIRGGDVKYNPVVLSYA
                     VITLKEVYLFVDESKLNEEILNELAKENVQIKPYNDVYEFVKNIDKTEKVLLDGTKLS
                     YTIYNNIPCEVEKVDEFNPVMFFKAQKNEVELENIRNSHVKDGVAFTKFMYWLKKNVG
                     KMEITEISATQKLEDLRREQEGFFEPSFNTIAAYKEHAAMMHYSATPESNYKLEAEGL
                     FLVDSGGQYYDGTTDITRTTVLGPISDELKLHFTSVARGMINLSKAKFLHGCRGYNLD
                     ILSRSCMWNMGIDYQCGTGHGIGFVLNVHEAPNGFRWRVVPERFDSAVLEEGMVTTNE
                     PGIYIEGSHGIRTENEIVVRKAEKNFYGQFMEFEVVTLAPIDLDGIVPELMNKDEKDY
                     LNWYHKLVYDKISPFLTDEEREWLKVYTRAI"
     gene            complement(9034..10416)
                     /locus_tag="ACOIAF_002555"
     CDS             complement(9034..10416)
                     /locus_tag="ACOIAF_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HGZ2669560.1"
                     /translation="MSVLKTQEKLKQPRDLYFCCSIFTFVGLAYYGMKLVLLLFLAEQ
                     VSKGGLGLTPAESASMLASFLAWTYFSPVIGGWVSDRFLGPKNCIILGMLMVSIGYFL
                     GYIANSEMDINIMIFLSGLGIGFYKGNLHTMVGNLYTNDDPRKDGAFSITYMATNLGT
                     LFGPLICGLVANEWFAMKSGSSISMYGYRYVFLFSSITVLIGLFLFIFGNRKFYKPLN
                     NDPSNGRESIRKSLEYKKMLASRPLTLIEKKRIIVILVLAFFTVFFWIAYNQASMSIA
                     LYTKEHINLTVGNFQIPTSWIDSYNGLLCVILGPISALVWVKLSQTKKGDLSVPKKMS
                     LGFILLAIAFLFMIVAVIQTGTDPNSIQKASVFWVVGFLTFQSIGEICFSPVGYGMVN
                     KLSPEKYISFLMGVWFLGKFAANKLSGYTQAIIDQLGMLQVFIVIPSFLLIFGIILLI
                     MNKKLVKLSN"
     gene            10674..11021
                     /locus_tag="ACOIAF_002556"
     CDS             10674..11021
                     /locus_tag="ACOIAF_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HGZ2669561.1"
                     /translation="MIRKKSELQVEVKENLNGGIGKVKLENIIQNEELKGKGRLFKRV
                     ALPVGSSIGVHDHTTDFEVYYILKGKGKVFDNGEFVEVNEGDVVYTADGEKHSIENIG
                     EEDLEFVALVLYM"
     gene            11039..12073
                     /locus_tag="ACOIAF_002557"
     CDS             11039..12073
                     /locus_tag="ACOIAF_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6398 domain-containing protein"
                     /protein_id="HGZ2669562.1"
                     /translation="MTGKIEEIKDKINRFCNSNLNQEYKDISFKILQNLLDNNKEIIH
                     SSRADIWSSAILNIVLEQNLLYNKKHPLHITKKEFSKQIGVSLNTINNKSSLIKDVCS
                     IDFLNQDTIAISNVEFWVKETNSKSKSVDLDLEKKKILYKRYIKLSQDANSDIESIRY
                     MEEAVNIGKSMITKEDKELGFWKGLSTRAYMIALESLARKLESINNLKESKKVLEYLI
                     EINPSDEQGIRYKLLNVLIRLNDRTSINKLFELYKEEKSATWMYSKALYYFKNKSMFL
                     ANDSIKVARSKNKYVGLYLLDWRNAFGREFVTEEEKAEAVYYYDENIVIWNEVKGSMD
                     WLLKKMLEFS"
     gene            complement(12122..13171)
                     /locus_tag="ACOIAF_002558"
     CDS             complement(12122..13171)
                     /locus_tag="ACOIAF_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HGZ2669563.1"
                     /translation="MEINYKKRNFKSFAKRKNIEISVQRYLIDALSYMALGLFSSLLI
                     GTIFNTLGDNFNISLFTEVISPLAKQVTGPAIAVAIAYGLQAPPLVLFSCALVGACGN
                     ELGGPVGAFVATLFAVEVGKLVSKETKIDILVTPAVTILIGVLVAELIGPAVSAFMTS
                     FGNIIMQATTMQPIIMGALVSALVGIALTLPISSAAICMMLSLGGLAGGAATVGCCCQ
                     MVGFAVMSFRENGVGGLVAQGLGTSMLQVPNIIKNWKIWIPPTVASILLGPLSTTIFK
                     MENIPIGSGMGTCGLVGQFGTVTAMQSAGKGGISMWVGILLLNFILPAIVTLLISEFM
                     RKKGLIKPEDLKLDV"
     gene            13446..14981
                     /locus_tag="ACOIAF_002559"
     CDS             13446..14981
                     /locus_tag="ACOIAF_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669564.1"
                     /translation="MIFVKNKKYRKIAIIISIPILLVAQYYLFKFGILRPMVKGVEIE
                     IVDGEYVKDIDKYVVKLNETVTLSTGEYIKVPSYAKNPNIWFNVLDNSGTLKIDGNKM
                     TALKVGYSSVGIMKNSRVLKKATIKVVDPEVESLDMEINGDLKHVGDSATIESTIEVD
                     YDKFKKSYKPVYKSSNENILKIKGNKVSAVGVGKATIYAKCGNKEIERTFRIQARVAE
                     INMDDIKIEVDQNEKLKPDIITSPPGLEPPKIYYELVDSKLPVERAISLSSNGNVVGL
                     REGKDRVRITCGDKSKVITVTVVKETITNDYIQNLVGSHKIVNNKVIIKLEWDYMKDV
                     FDYDIYLKDKLSGESFKKIKSITINESDLGKSNKVYATIEVDMKGNQNIDFDIYVIGK
                     KDSDTTKPSNVINITNGNGNSNNDNNIKDMRVENISANVDRDNNVIRVNWSSISHKDC
                     TYSIYVKNNTNGDGEFTLYQSDIQGNEHTISIPEGDLNLDIYVVANYNGNSSQNSDVI
                     NVR"
     gene            complement(15166..15852)
                     /locus_tag="ACOIAF_002560"
     CDS             complement(15166..15852)
                     /locus_tag="ACOIAF_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HGZ2669565.1"
                     /translation="MKTIGLIGGMSWESTITYYQVINTVIKERLGGLHSSKCILYSVD
                     FQEIEECQSSGNWEKSAKILADAAIKLQEAGADFIVICTNTMHKVSDKIQESIHIPLL
                     HIADVTATVLREKEIKKVALLGTKYTMEQDFYKNVIINNGIEVLIPNEEDRIIVNDTI
                     FNELCLGIISESSKKAFLSIIDKLGKQGAEAVILGCTEIGLLIKQNDTSIPLFDTTVI
                     HAIEAALSSI"
     gene            15981..17363
                     /locus_tag="ACOIAF_002561"
     CDS             15981..17363
                     /locus_tag="ACOIAF_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HGZ2669566.1"
                     /translation="MPINSFENYPMTWKPKLDNRKPPIYKTLAMLLEEDIKRGNLNPG
                     DKLPPQRELADFLDLNLSTITRAFKLCEEKGLICAKVGKGTFISSDVNVSNTLLYQTE
                     SKDIIELGTVHPPYEQNTYIIDFIKNVLKQPEMDRFLQYMSPSGTYMQKKSIAKWIER
                     NNVYTSEENILLSTGGQNAICATLLGLFKAGDRIATDSLSFSGIKSIAKMIGIQLVPI
                     PQENNEISIEYLESYCKNENIKGIYLIPDYHNPTTHTMSDLSRKKIAKIAKQYNIIII
                     EDAINSIFRDGIQTPIFSLASDNTIYIFSTSKFLCAGLRVAFVVAPKRYIENLENALY
                     NMNLMVSPFTAEIVHRLLYSPIIDKIIEEKRDAIIERNEVADKVLSDYNLIGDKNCSF
                     RWLLLPDELDGKSFEISAKNLGVQVYCAERFSVGNSTVPKAVRICVTAPKDVEELEKG
                     LNIIKSLLKS"
BASE COUNT         6876 a         1914 c         2950 g         5687 t
ORIGIN      
        1 tataatgata ttaatataac caagcgattt ttaatttagc tttttattaa gcttattttt
       61 tatactattt ataataaata aactccatga tgggaaaact attactattt aatatgtaaa
      121 ggagtatttt aaatgaatga atcatattgg tttttaaact caagtccaaa agaatataac
      181 aaattaggtg aaaatataaa aacagattgt cttatagtag gtggtggaat aactggattg
      241 acaactgcat atttattagc aaaagaaggt aaaaaagtag tgcttgttga ggctgataaa
      301 ataggatatg gaacttctgg aaggaatact ggtaaggtaa cttgtcagca tgatatattt
      361 tactctaaga tagagaaaaa atatggatta gataaagcaa aatcatatta taatgcaaat
      421 aatgaagctt taaatttagt tgaacaaata atagaagaaa acaatataaa atgtgatttt
      481 aaaagggaaa cttcttttgt atttacagaa aaagaggata ctataaagaa tattaaagat
      541 gaatatcgta catgtaaaaa aataggaata aattgtgagt atcatgaaac tatagaaaat
      601 attccattag atataaaggg ggctattagt tttactaatc aaggtcaatt taacccaaag
      661 aagtatattg atggattggc aaaagcagca gtaaatttag ggctaaaaat atatgaaaat
      721 acaccagtag tagaccttga aaaaggaaaa atatgtaggg ttaaaacaag agaagataat
      781 ataatagaag ctgagaatgt tataatttca tctcatagtc catggtatga tggattgaat
      841 ttttattttg caaaagaata tgcagagcgt gcttatttaa tggttgcagt tttggaaaat
      901 aaattagcag atggtatgtt tataagtata gatgacccat caataacttt tagacaatat
      961 aatgatggaa gtgaaaattt actgattttt ggaggaggag accataaagt tggtcaggga
     1021 ggaactgaga aagaaatatt tgatgattta gagcattatg gtaaagaagt ctttaaggta
     1081 aaagatttta aggggaaatg gtctgctcaa gataatatga gttttgacaa tgttccttat
     1141 attggttata tcaacaaaag agaagacaat atttatgtag ctacagggtt ctctaaatgg
     1201 ggaattacta atggaactgc tgctggaata ataataaaag atttaataat aaataataat
     1261 tctgattata aagatacttt taatccatcc agactaggta gctacttttc aaaagatttt
     1321 ataaaggaaa atgcaaacgt agcaataaat tatgtaagtg gaaaattaaa aataggtagt
     1381 ggagatatgc ctaaaaataa tggagaagga aagattgtta atattgatgg aaaaagatat
     1441 ggtgtatata aggatgataa tggagatttt tatattgtag acactacatg tactcattta
     1501 ggatgtgaac ttaattttaa cagtgaagaa aaaacatggg actgtccatg tcatggttct
     1561 agatttgact ataagggaaa tatacttgaa ggaccagctc ttaagccatt aaaactttat
     1621 ggacatggtg ataatgatgt taatcctaag ttattataaa attattttta ggaaggtaaa
     1681 gtgtatatga ataaactttt aaagccaata actatactat tgtatatagt ttatatttta
     1741 tttgccgtat tttttgtagc aaacaaagaa tatggaagtc taggtctagt agcaattagt
     1801 ttagttggta ctttcatttt gagttttata aataagcaaa ctgaaagatt attgagtaaa
     1861 gaattatata taatgcttgc tttatttata atgttttcat cattgttagg tacttgtttt
     1921 aatttttatg atataaatta ttatgatgat tttttgcatt tatggtcagg tataatatct
     1981 tgtattgtgg ctttttctat aattaaatac ttttatacac aaagagagat aagtaaaatg
     2041 agtaaagtgt ttttagttat atttttattt atgtttagta tgggagtagc gagtttatgg
     2101 gaaataggtg aatttttaat ggatactttt gttgggacta ctacacaggc cggagggtta
     2161 gaagatacta tgattgatat ggtagatgct ttaataggca caattataac tattcctttt
     2221 atagtaaaaa aatctaatat atagttttaa attttttata ttaattataa tggatttaat
     2281 taatcatgtt gagtttcaag gtctatatga ttaattaaat ccatttttgt tatttataat
     2341 tattattgaa tatattttta catataagat ataaggtgat agatagaaat tgttggatta
     2401 tgtaaataat taatatataa taccattatg ggcaataaat acaaatcaaa tgataatata
     2461 gtctatttat gtaaatgtta tattgtatcc taagtataat agtaagtttt ttgatagtgg
     2521 tgtagaaagt aaaaatctac tagcctaaat tgatgtatgg attagtttag gaacagtaat
     2581 tgcatatctt aagtttgatg tcatgttggg ctactagaaa taggtcatct ttttcacttg
     2641 aatatctttt ctttaaacat ggggagtatc atatcaaaaa catatgaaaa ttaagtgatt
     2701 tatgagaaca gtaatatttt atatgatagt atagaatagt aataaagtat taggaggaac
     2761 attgagtgtt aaaaaagtgg tttggtattg tgaaaaaaag acaaaaaagt gagtcagtaa
     2821 aagaagaagg tgaagtaata ttaaaaaacg aaaaaatatt atctgaagaa aagttgatag
     2881 atgaagaagg agttgtagtt aatattgata atgaaatatt aactaaagta gaagtagtaa
     2941 atgatgacaa tgaaatagaa gaaaaaataa tagaagaaga tttgttaatt agtgaaaata
     3001 ccataaaatt agatgataaa gaagcaatta ttaatgataa aaacatagaa ttatgtaaaa
     3061 aagaagttca agttgaaagt gaaaaaatag gtttaaataa gtttgaagga ctggaccaaa
     3121 atgaaaatta taatctagaa aaaaatgtta ttgaagaaaa aaaagtaagt gaatgtttga
     3181 cagaagaaga tttagagtac ataaaagaaa ttaaaataaa aagaggaaaa agtataaaag
     3241 ctataaactt gtatactaaa gaagagtggg tttttgacac tcatatacag tgtagtaaaa
     3301 aactcaaagt tccattaggg tacataagag aaaatttaaa atatggatat atggattact
     3361 ttggagatgc aataaattat ttaagtgaag tattaaatat agatgaatac tgcaaaagtg
     3421 aatggagcta tctcgataat agcaaatctc catctgagat atttaatatt ctaaacaata
     3481 aaatatttag tataaggctt tctaatgaaa aaagaaatga aattttgaca aatgataaaa
     3541 ttgaagcatt aaaaatgaat tatagatttg aatgtattga tgaagaatat gatgaatatt
     3601 ttaaaaaata taagtctata atcaaaagag gtggaaagaa aaaagttgaa ttagtaaata
     3661 aaaaaggtga cattttagaa atatttaagt ctttagaaga atgtgccatt tatttgcaaa
     3721 aggaaaagaa tgaagttata caaatgttaa aatatggaga tacaaaagta ggaagaaact
     3781 ttataaggta tagtttgaga agtatttaat ttcattttaa attcataaga gtttcatttc
     3841 tgattcacac atttaatatt attttaatgt aaaataataa aggtgaagta ttgcacaatt
     3901 aaaattaagg agggtattaa tgtctgaaga aaaaaatcca aaagaaaata cattagatga
     3961 acctaaaaat tcagataatg taaattctaa aaaagatgat gtgtcagatg aaaaaaatac
     4021 tgataacaaa gttaaaaaaa gggaaataaa gctaaacaac aaaacactta aagctattgt
     4081 agcaattgtt gcagtaggag ctatatgttt aggtggaggc tatgtttttg gcaaagaagc
     4141 tggaagaaaa ttgcctgcta cttcaaaaca ctataatagc agtgaggtag tagctactat
     4201 tggggataaa aaaataacag gtgaaatgtt aagaaagaga atggagccat tattttattt
     4261 aaatgcaaaa actactatga ctgatgacga aatacgttca tatgaatcaa atatgattga
     4321 aactatgatt acatctgata tattatcctc tgaagcagca aaagagaaaa taagtataac
     4381 taatgaccaa ctagatgctc agtataataa tttaatgagt agtatagaat cagcaatggg
     4441 aatgactaaa gaagagtttt taaaacagtt tgatttgact gaaaaatata taaaagatga
     4501 tatgaaaaaa gagctagtag catcaaaata tttaaatgaa aaatcaaaag ttactgataa
     4561 agaagcagag aactattaca ataaaaacaa gagcaattat ttacaagtta gagcttcaca
     4621 tattttaata aaaactgttg atgataaagg taagcaagtt agtagtagca aaaaagctga
     4681 acttaagaaa gaagctgaag aaatacttaa aaaagcccaa gctggggaag attttgctac
     4741 tctagctaaa aaatactctg aagatagtag tgcagaatca ggtggagatt taggtttctt
     4801 tggtaaaggt cagatggtgg aatcttttga aaaagcagcc tttgcattga agaagggaga
     4861 ggtatctaac aaacttgtag aaagtgatta tggatatcat ataattaaga aaactgatga
     4921 aaaatatcag ccatttgaag aggtaaaatc agatttagta agtagtttaa catcagaaaa
     4981 acaaaaccta cttatacaaa atctaaaaga aaaatataat gttaaaatta cagataaata
     5041 tcaaatgaaa gctaaagaat agataaagtc aatttattaa aaaaaagaaa aattaactgt
     5101 atggataaaa gctattttta ctctcaatat agagagtata aaatagcttt tattattttg
     5161 caataaaatc tatttatata tatgaagtga caaagaaata acatgcaaaa agatattaaa
     5221 aagaaatatt tttaatttga attttttcaa taaaaattta caaaaatgaa aaaataaaca
     5281 aaaaagaatt gaagatttaa gttgttattt aaatatatat atattaagat tttctgctga
     5341 agatactaaa aaaataatat ttttcataat tttaaaaata ataataaaaa aatgaaaata
     5401 caggcgataa tctattgagt aaacatagaa aagatgatat tataagtatg tgctaaaatg
     5461 gttaaagcaa tattaatatt aaatttaact ataaaacaaa agggggcgtt tttttatgtc
     5521 aagtacagaa agcgtatcta aaaaaagtgg tcatccgaag agtttttggc ttatttgcat
     5581 gacaattttg tgggaaagat ttagttatta tggtttaaca gctttattgg tattattttt
     5641 tacagcaagt gtagctcaag gtggtttagg tctttctact caagaagcaa catccatgtt
     5701 tggcttattt acaggtctta tatatcttac accattagtc ggtggctggt tagcagataa
     5761 agttttaggt caacaaaaat gtatttctat aggtgcactt ttaatagcaa taggagattt
     5821 cgtactattt gcttcatcta catctattgc aatggtttgg gtaggtctat caataattat
     5881 tttaggaaat ggttttttta aatcaagttg ctcaaatata gttggggata tatatcctaa
     5941 aactgatgtg tcaagaaaag atgcagcata cagtttattc tatatggcag taaatgtagg
     6001 agcatttata gctccaataa tatgtggatt gctttctgat aatgtatttg cagtaagagg
     6061 agcaagtgga gatatagtat cttatggata taagatggca tttttaattt gtggtatagg
     6121 catgatgtta ggtatgatta tatttacact tcttgcacca aaattattaa atgaagtcgg
     6181 aaaatatcct gtgactaaag gtaataaagg tgaaaaaatt gagtatggac cacttactaa
     6241 gttggagaaa aataggataa tagcaatggt tattttattt ttatttgtgg tcttattctg
     6301 gacagcatac tatcaaacac aaagttcttt tatgatttat gtaagggata atgtaaatag
     6361 aactctattt ggatttgaaa tgccagtagc atggcttaca tcactaaatg ctatattatg
     6421 tgtggcttta tgcccagtat tagctaccct atgggttaaa ttatctcata ccaaaagagg
     6481 tgatttctct attccagtta aaatgggatt aggaatgata ataatgggtg ttgggtttat
     6541 agttatggta tttgctacat tagctaatgg tccagatgga atggtaaagg ctagtatatt
     6601 gtttatagta ttgacttata tatttacaac agtaggtgaa ttattcttat ctcctatagg
     6661 acttgcaatg ttcaataaat tagctccagc taagtatgct acgttatcta tgggagcctg
     6721 gtatctaaca agtttttttg caagtttatt atcaggaaag attgctggtt ttacagccac
     6781 acttggtttt ttacagatat ttggaggaat agcaacagtt ttaataatat ttggtttaat
     6841 attattggga ataagaaatg tattaacaag attaatggct atggatttac taacaaaaga
     6901 aaaataattc ataacgaatt attgtaaata aatgaattac tttaaataaa ggaagttaca
     6961 tttaaatata aaattaaatg taatttcctc tttgattatg aaaaaaagct ttacacaaat
     7021 agatattata attaaaatta taattgtgat aattagagtg tataagattt atataaatta
     7081 tacaaaaaat atttatgtaa taaactttac atacttatat atttagttat tcaaactatt
     7141 atttcgagga ggtcttgttg tgaatataaa agatagatta agtggattaa gaaaatttat
     7201 ggaagaaaaa aatatagatg cctatatgat tccatcttct gataatcacc aaagtgaata
     7261 tgtaggagat tacttcaaat caagagaatt tatatcaggg tttaatggtt cagcagggac
     7321 ggttattgtg actaaagacg aagctgggtt atggacagat ggaagatatt ttatacaggc
     7381 agaatctcaa ttagaaggaa gtacaataaa attattcaaa atgggacaag aaggttgccc
     7441 aactacagat gaatatttat ataaaaatat tcctgaagga ggaactttag gatttgatgg
     7501 tagagttata tctgctagag aaggagctac attagctgaa aaattatcta aaaaaggtat
     7561 aaagatagaa tatcagtatg atttaataga tagtatatgg ccagatagac ctgctttatc
     7621 agatagtaaa gcatttttat tagatgttaa atattgtgga gaaagtttta gctcaaaatt
     7681 agctagatta agagaaaaaa tgagtgaaaa aggtacttct actcatgtaa taactactct
     7741 tgatgatata gcatggttat tcaatataag aggtggagat gttaaatata atccagttgt
     7801 actttcttat gcagtaataa ctttaaaaga agtttatcta tttgttgatg aaagtaagtt
     7861 aaatgaagaa atactaaatg aactagctaa ggaaaatgtt caaataaagc catataatga
     7921 tgtgtatgaa tttgtaaaaa atatagacaa aactgaaaaa gttttattag atggaactaa
     7981 gcttagttat acaatttaca ataatatacc ttgtgaagtt gagaaagttg atgagttcaa
     8041 tccagtaatg ttttttaaag ctcaaaaaaa tgaggtagaa ttagaaaata taagaaattc
     8101 acatgtaaaa gatggtgtag ctttcactaa atttatgtac tggttaaaga aaaatgtagg
     8161 taagatggaa atcactgaaa taagtgctac tcaaaaatta gaggacttaa gaagagagca
     8221 agaaggtttc tttgagccaa gtttcaatac aattgctgca tataaagagc atgctgcaat
     8281 gatgcactat agtgcaactc cagagtcaaa ttataaatta gaggcagaag gattattttt
     8341 agtagattca ggtggacaat attatgatgg aactactgat ataactagga ctacagtttt
     8401 aggacctata agtgatgaat taaaactaca ctttacttct gtagcaagag gaatgataaa
     8461 tctttctaag gctaagttct tgcatggttg tagaggatat aacttagaca tactatctag
     8521 aagttgcatg tggaatatgg gaattgatta tcaatgtgga actggacatg gtataggatt
     8581 tgtgctaaat gttcacgaag ctccaaatgg atttagatgg agagtagtac ctgaaagatt
     8641 tgatagtgca gttcttgaag aaggaatggt aactactaat gaaccaggta tatatataga
     8701 aggttctcat ggtataagaa cagagaatga aattgtagtt agaaaagctg aaaagaattt
     8761 ctatggtcaa tttatggaat ttgaagttgt tacgttagct cctattgact tagatggtat
     8821 agtaccagaa ttaatgaata aggatgaaaa agattattta aactggtatc ataagttagt
     8881 ttatgataaa atatcacctt tcttaactga tgaagaaaga gaatggttaa aagtttacac
     8941 tagagctata tagtttgtac tagatacaat aaaaatcgag gtattataat gcctcgattt
     9001 tttattgtat attttattaa ctttaaaaaa tttttaattt gataatttta ctaatttttt
     9061 attcattata agtagtataa ttccaaatat caataaaaat gatggtatca ctatgaagac
     9121 ttgtaacata ccaagttggt ctataatagc ttgcgtatag ccagataatt tatttgctgc
     9181 aaatttgcct aaaaaccaaa cacccattag gaatgaaata tacttttcag gcgaaagttt
     9241 atttaccata ccatacccga ctggagaaaa gcaaatttct cctatacttt ggaatgttaa
     9301 aaatccaact acccaaaata cacttgcttt ttgaattgaa ttagggtctg ttcctgtttg
     9361 tataactgct actatcataa acaaaaatgc tatagctaat agtataaaac ctaagctcat
     9421 tttttttggc acacttaaat cgcctttttt agtttgagat aattttaccc atactaaagc
     9481 agagataggt ccaagaataa cacaaaggag accattgtat gaatctatcc atgaagtagg
     9541 aatttgaaaa ttgccaacag taagatttat gtgttccttt gtatagagtg caatagacat
     9601 agaggcttgg ttatatgcta tccaaaaaaa tactgtgaaa aaagctaaca ccaatattac
     9661 tattattctt tttttctcaa tcaaagttaa aggtcttgaa gcgagcattt tcttatattc
     9721 tagtgatttt cttatagatt ctctcccgtt actagggtca ttattcaaag gtttataaaa
     9781 ctttctgttt ccaaatataa agaggaataa acctattaga accgtaattg atgaaaataa
     9841 gaatacatat ctatatccat acatacttat agaggatccg cttttcatag caaaccattc
     9901 attagcaacc aaaccgcata ttaaagggcc aaataaagta cctagattag tagccatgta
     9961 cgtaatagag aaagctccat cttttcttgg gtcatcattg gtatataagt ttccaaccat
    10021 agtatgtaag tttcctttat aaaaacctat tcctaatcca gataaaaata tcatgatgtt
    10081 tatatccatc tcactatttg ctatgtatcc tagaaaataa ccaattgaaa ccattagcat
    10141 tccaagtata atacagtttt tgggacctaa aaatctatca ctgacccatc caccgataac
    10201 tggagagaaa taagtccatg ctaaaaaaga tgcaagcatt gatgcggatt ctgctggtgt
    10261 taaccctaaa ccacctttgg agacttgttc agctaaaaac aataataata ccaatttcat
    10321 accatagtat gcaagtccta caaaggtaaa tattgaacag cagaaatata aatctctagg
    10381 ttgtttaagt ttttcttgcg tttttaaaac tgacatatgt tgagaacctc cagataaata
    10441 gtttaacaaa attttaccat actatatgag tatatgtttt tttcatatag acgtcaactt
    10501 atgagataat ttaattaagt cgaaatatat gtaaaaatag tatcattttt gtgtattcag
    10561 taatattttt ttatgataat atatgttatt attatattaa aatacaaaca tatttatgga
    10621 aaggatatgc caatttagta gttagattga ttgtttggca ggtgggatga gctatgataa
    10681 gaaagaaatc tgaattacaa gtagaagtaa aagaaaattt aaatggtggt ataggaaagg
    10741 ttaagcttga aaacataata caaaatgaag agcttaaagg aaaaggaaga ctatttaaaa
    10801 gagtagcgtt accagtagga agttcaattg gtgtgcatga ccatacaaca gattttgaag
    10861 tatattatat actaaaggga aaaggtaaag tatttgataa tggggaattt gtagaagtaa
    10921 atgagggtga tgtagtgtat acagctgatg gcgaaaaaca ttctatagaa aatataggag
    10981 aagaagatct tgagtttgta gcactagttt tatatatgta aaatatggag gaggagtcat
    11041 gacaggcaag atagaagaaa ttaaggacaa gataaataga ttttgcaact ccaatttaaa
    11101 tcaagaatac aaagatataa gctttaaaat tttgcaaaac cttttagata ataataaaga
    11161 gataattcat tcttctagag ctgatatatg gtcttcagct atactaaata tagttttaga
    11221 acaaaatctt ttatacaata agaagcatcc actacatata acaaaaaaag agttttcaaa
    11281 acaaataggt gtttctttaa atactataaa taataaatcg tctttaataa aagatgtttg
    11341 tagtattgac tttttaaatc aagatactat agctatttct aatgtagaat tttgggtaaa
    11401 agagaccaat tccaaatcaa agtctgtaga tttagattta gaaaagaaaa aaatactata
    11461 taaaagatat attaagctat cacaagatgc gaatagtgat attgaatcaa ttagatatat
    11521 ggaggaagct gtaaatatag gtaaaagtat gattactaaa gaagataaag aacttggatt
    11581 ttggaaaggt ttatctacca gagcctatat gatagcactt gaatcccttg ctagaaagtt
    11641 agaaagtatt aataatctaa aggagtccaa aaaagtatta gaatatttaa tagaaattaa
    11701 tcctagtgat gagcagggaa taagatacaa attattaaat gtattaatta gattaaatga
    11761 tagaacttca ataaataaat tatttgaact ttataaagaa gaaaaatctg ctacatggat
    11821 gtacagtaaa gctctttatt actttaaaaa taaaagtatg tttttggcaa atgattctat
    11881 taaggttgct agaagtaaaa ataagtacgt tggattatat ttgttagatt ggcgaaatgc
    11941 ttttggtaga gaatttgtta cagaggaaga aaaggcagaa gctgtctatt actatgatga
    12001 aaacatagtg atttggaatg aagttaaagg ttctatggat tggctattaa aaaaaatgtt
    12061 ggaattttca taaaaaaaac tactccttac ttttatataa gtcaaaggag tagtttttaa
    12121 tttatacatc tagttttaaa tcttcaggtt ttattagacc tttttttctc ataaattctg
    12181 aaatcaacaa agttacaata gcaggaagta taaaattaag taaaagtata ccaacccaca
    12241 tagatatacc tccttttcca gcactttgca tagcagttac tgttccaaat tgtccaacca
    12301 atccacaagt acccatacca gaaccaatag gaatattctc cattttgaat attgttgtag
    12361 ataatggtcc aagtaatatt gaagctacag taggtggtat ccaaattttc caatttttta
    12421 ttatattagg tacttgcaac atagatgtcc caagaccttg tgccacaagg ccaccaactc
    12481 cattttctct aaagctcata acagcaaagc ctaccatttg gcaacaacaa cctacagttg
    12541 ctgcaccacc agcaagacca cctaaactta acatcataca aatagctgca ctacttatag
    12601 gtaatgtaag agctattcca acaagtgcag agactaaagc tcccattatt ataggttgca
    12661 tagttgtagc ttgcattatt atatttccaa atgaagtcat aaaagcagaa acagctggac
    12721 caataagttc agctacaagt actccaatta gtattgttac agcaggtgta accaatatat
    12781 ctatttttgt ttcttttgaa actaattttc caacctctac agcaaaaagt gtagcgacaa
    12841 atgctccaac aggtcctcct aattcattac cacaagctcc aacaagtgca caagaaaata
    12901 gtacaagtgg aggtgcttga agtccatacg caatagccac tgctatagct ggaccagtaa
    12961 cctgtttagc aagagggctt ataacttcag taaaaagaga aatattgaaa ttatctccta
    13021 atgtattgaa aattgttcca attaaaaggg acgaaaaaag accgagtgcc atataactta
    13081 gtgcgtcaat aagatacctt tgaacagaaa tttcaatatt ttttctttta gcaaatgatt
    13141 tgaaatttct ttttttataa ttgatttcca atttaaaatc ctcctcatat aatataagta
    13201 taatttcttt ttaattaatt atataacttt tcagctgaca agacaaatgt tatttcacta
    13261 aaattagact attgtaatag taagaaatta acattaattt tagttttaaa ttttaaattt
    13321 atggtttata attagtatag ggatattctg aaatataata attttccagt ttatcccaat
    13381 atttaactta ggtagattgc ttacaaaaca aatgattata tattaggcta aaaggaagtg
    13441 gatagatgat atttgtaaaa aataagaaat acagaaaaat tgccataatt atatctattc
    13501 ctatattgct tgttgcgcag tactaccttt ttaaatttgg aatacttaga ccaatggtaa
    13561 agggagtaga aattgaaatt gtagatgggg aatatgttaa agatatagat aaatatgtag
    13621 taaaattaaa tgaaacagtt actttgtcta caggtgaata tattaaggtt ccttcgtatg
    13681 ctaaaaatcc aaatatttgg tttaatgtat tggataactc agggacttta aaaatagatg
    13741 gaaataaaat gacagcatta aaggttgggt attcttctgt gggtatcatg aaaaattcaa
    13801 gagtattaaa aaaggctact ataaaagtag tagatccaga ggtagaatca ctagatatgg
    13861 agattaatgg ggatttaaaa catgttggtg atagtgctac tatagaaagt acaatagaag
    13921 ttgattatga taagtttaaa aaatcatata agccagttta taagagtagc aatgaaaata
    13981 ttttaaaaat caaaggaaat aaagtaagtg cagttggagt tggtaaagct acaatatatg
    14041 ctaagtgtgg aaataaagaa attgagagaa catttaggat tcaagctaga gttgctgaaa
    14101 taaatatgga tgatataaaa atagaagttg accaaaatga aaaattgaaa ccagatataa
    14161 taacttctcc acctggttta gaaccaccaa aaatatatta tgagttagta gattcaaaat
    14221 tacctgtaga aagagcaata agtttaagta gtaatggaaa tgttgtaggt cttagagaag
    14281 gtaaagatag ggtgagaata acttgtggag ataaatctaa agtaataaca gtaacagtag
    14341 tcaaagaaac tataacgaat gactatatac aaaacttggt tggttcacat aaaatagtaa
    14401 acaataaagt tattattaaa cttgaatggg attatatgaa agatgtcttt gattatgata
    14461 tatatcttaa agataagttg tcaggagaat catttaagaa aattaaatct ataactataa
    14521 atgaaagcga tttaggtaag tctaacaagg tatatgcgac tatagaagta gatatgaagg
    14581 gtaatcaaaa tatagatttt gatatttatg tcatagggaa aaaggattca gatacaacta
    14641 agccaagtaa tgtaataaat attaccaatg gaaatggaaa ttcaaataat gataataata
    14701 ttaaagatat gagggtagaa aatatatcag ctaatgttga tagagataat aatgtcatta
    14761 gagttaactg gagtagtata tctcataagg actgtactta cagtatatat gtaaaaaata
    14821 atactaatgg agatggagaa tttactctat atcaaagtga tatacaggga aatgagcata
    14881 ctataagtat tcctgaagga gatttaaatc tagatattta tgttgttgca aattataacg
    14941 gaaatagctc tcaaaatagt gatgttataa atgtaagata gatttgtata caaattgaaa
    15001 gtgttgagtt tatttaaata ttaaattaag aaaaaggata gtatagggta attatactat
    15061 ccttttttgt tacaatatat aggtttaaaa tctctagtaa tataaattat aaaaagtctt
    15121 atttacacaa atctatagat atttttttta ttaatcaata ttgtcttaaa ttgaacttag
    15181 tgcggcttct attgcatgta ttactgtcgt atcaaatagt ggaatagaag tgtcattttg
    15241 tttaattaaa agacctattt ctgtacatcc taatataaca gcctctgcac cttgtttgcc
    15301 aagcttatct ataatagata agaaagcttt ttttgaagat tcagatataa ttcctaagca
    15361 aagttcgtta aatattgtgt catttactat tatacggtct tcttcatttg gaattaagac
    15421 ttcaattcca ttattaataa ttacattttt ataaaaatct tgttccattg tatatttagt
    15481 acctaataag gccacttttt ttatttcttt ttcccttaat accgtagcag ttacatctgc
    15541 tatgtgaagt aatggaatat gtatactttc ttgtatttta tctgatacct tatgcatagt
    15601 attagtacag ataacaataa aatctgcccc tgcctcttgt aatttaattg cagcatcagc
    15661 taaaattttg gcactttttt cccaatttcc actagattgg cattcttcaa tttcttgaaa
    15721 atctacactg taaagtatac atttagaaga gtgtagtcca cctaatcttt cttttattac
    15781 tgtattaata acttgataat atgtaatggt actttcccaa ctcataccac caattaagcc
    15841 tatagtcttc atattatacc tctcttattt atttatttat tttgaaatag ctatttacaa
    15901 atacaagtat agcatctaaa atagaagcat acaataataa aattgtatgg agtacaatta
    15961 taattaagag gagtaattat atgccaataa attcttttga aaactatcct atgacatgga
    16021 aaccaaaatt agataaccga aaaccaccaa tatataaaac tttagcaatg ttattagaag
    16081 aagatattaa aagaggaaat ttaaatcctg gagataaact gcctccacaa cgggaattag
    16141 cagattttct agatttaaac ttaagtacaa taacacgagc atttaaactt tgcgaagaaa
    16201 agggactaat atgtgcaaaa gtgggaaagg gaacattcat ttcttctgat gtaaatgtaa
    16261 gtaatacatt attatatcaa acggaatcaa aagacataat tgaattaggg acagttcatc
    16321 ctccatatga gcaaaataca tatattattg actttattaa aaatgtacta aaacaaccag
    16381 aaatggatag gtttttacaa tacatgtctc catctggcac atatatgcaa aaaaagtcaa
    16441 ttgcaaaatg gatagaaaga aataatgtat acaccagtga agaaaatata ttattatcta
    16501 caggagggca aaatgctata tgtgcaactt tactaggatt atttaaagct ggtgatagaa
    16561 ttgctacaga ttctttaagt ttttctggaa ttaagtcaat agctaaaatg ataggtattc
    16621 aattagtccc aattcctcaa gaaaataatg aaatatcgat tgaatatctt gaaagttatt
    16681 gtaaaaatga aaatataaaa ggcatatatc ttataccaga ttatcataat ccaacgacac
    16741 atactatgtc tgatttatct cgtaaaaaga tagccaagat agcaaagcaa tataatataa
    16801 taattattga agatgctata aatagcattt ttagagatgg tatacaaact cccatatttt
    16861 ctttagctag tgataatact atatatattt ttagtacatc taaattttta tgtgctggtc
    16921 ttagagtagc atttgtagtt gctccaaaac gatatataga aaatttagaa aatgcactat
    16981 ataatatgaa tctaatggtt tctccattta cagcagaaat agtacaccgt ttattatatt
    17041 ctcctattat agataaaatt atagaagaga aaagagatgc aattatagaa agaaatgaag
    17101 ttgctgataa agttttgtca gattataatt tgattggtga taagaattgc agttttcgtt
    17161 ggttacttct tcctgatgaa ttggatggaa aatcatttga aatttctgca aaaaatctag
    17221 gagttcaagt atattgtgcc gaacgatttt cagtaggaaa ctctacagtt ccaaaagcag
    17281 ttcgtatttg tgtaacagct ccaaaagatg tagaagaatt agaaaaaggg cttaatataa
    17341 taaagtcact tcttaagagt taaatttatt ttactattat ttaattaaat aaataaaaag
    17401 gcggagaaat ttattataat aaacctc
//
LOCUS       DBGJFF010000080        17059 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.081, whole genome shotgun sequence.
ACCESSION   DBGJFF010000080 DBGJFF010000000
VERSION     DBGJFF010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17059
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.081"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            280..735
                     /gene="smpB"
                     /locus_tag="ACOIAF_002562"
     CDS             280..735
                     /gene="smpB"
                     /locus_tag="ACOIAF_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HGZ2669567.1"
                     /translation="MAGKKILATNKRARHEYFIEETYECGIELKGTEVKSIRQGKVNL
                     SDGFASVDNSEVFLKQVHISPYEQGNIFNVDSLRVRKLLLHKHEIRKLIGATTIKGYS
                     LIPLSLYLKNGKVKVEIALAKGKKLYDKRQDMAKKDAQRRIEREMTGKY"
     gene            893..1242
                     /gene="ssrA"
                     /locus_tag="ACOIAF_002563"
     tmRNA           893..1242
                     /gene="ssrA"
                     /locus_tag="ACOIAF_002563"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            1574..1789
                     /locus_tag="ACOIAF_002564"
     CDS             1574..1789
                     /locus_tag="ACOIAF_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3791 domain-containing protein"
                     /protein_id="HGZ2669568.1"
                     /translation="MDERKLEFSIFCIEALAEKLNIDSEGVYKLLKHDTDILDNYIIP
                     CYEALHSQSKGYIVEDLMQVLKEKGVI"
     gene            1789..2268
                     /locus_tag="ACOIAF_002565"
     CDS             1789..2268
                     /locus_tag="ACOIAF_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3990 domain-containing protein"
                     /protein_id="HGZ2669569.1"
                     /translation="MIVYHGSYCVVKTPSITFSRDSLDFGKGFYVTSIKTQAINWTER
                     FKKRGKKAYLNSYSLDIDKLKENCKVKIFDSYNLEWLDFIIECRNMSDIYMKYDIIIG
                     GIADDKIYNTIELYQDNLIEKDEALKRLKYYKPNEQICITNQNVIDKYLKFRYEEEV"
     gene            2274..2504
                     /locus_tag="ACOIAF_002566"
     CDS             2274..2504
                     /locus_tag="ACOIAF_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669570.1"
                     /translation="MEANKTLLQILYKKIILEFSKQTGKSLEESMDYLYTSETYKLIS
                     EGVSDMHCKGHIYLAQELMLENGLMYHKGYPR"
     gene            complement(2620..3786)
                     /locus_tag="ACOIAF_002567"
     CDS             complement(2620..3786)
                     /locus_tag="ACOIAF_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGZ2669571.1"
                     /translation="MATKRANGEGSIGKYKDGWRSRIMIGYNEDGKPLRKEFYGKTQK
                     EVKDKLDAYKKQYYLSSDISDDKITLEHWFYNWLFEYRIKDLKPKSFERYESIYRNYI
                     KDTDLGNVKLKYLRVSHIQKYYNRLLDSNKSIPTIRQINTKLKTCLSEAEKQGLIQRN
                     YCTMVNIPVEKKENKLEILTLEQQKSFIKAIDGHKLEVLFLLALGTGLRLGELLGLKW
                     FDIDFKKSNLTVKRTLQRTYFIDKTGNRELKVLEQGPKTSNSYRTVPIPKDVLNKLKE
                     HKVNQNTDILKAGELYINNNYVFCNELGIPIDDKRPLRNLKSILNSLNIEPIKFHGLR
                     KTYATRLFENDVPPKTVQVLMGHYDISITLNIYTQVMEDKKVEAVEKLDKIFSL"
     gene            complement(3776..4528)
                     /locus_tag="ACOIAF_002568"
     CDS             complement(3776..4528)
                     /locus_tag="ACOIAF_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HGZ2669572.1"
                     /translation="MNKSYIPKYTHKFPTNIPHGDKKYTLTYIRRMISEFVYDMGNLE
                     NNPFTFPEVQTLLDGITVGGHKLSDQNQILNIKSSWDYVIKLSNKENLSLNKDTICSI
                     HKIVAKDEALIVGDFRNGNIGIAGTTQYECIEATLLNDLFVNDINIINKITNPLEKAI
                     IINLWLSYCQFFYDGNKRTARLTSNLILINNDIGVLSIPAKHKQEYNTLMLNFYETLE
                     ADEVIKFLLEKCITFFDGYNYKSYKELFKNGN"
     gene            complement(4620..5108)
                     /locus_tag="ACOIAF_002569"
     CDS             complement(4620..5108)
                     /locus_tag="ACOIAF_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGZ2669573.1"
                     /translation="MNNTIGEKIKQYRKSINMSRDELANKIGCSVHAIAKYEQNQRMP
                     SLDMIRKIATALSIAINKLIVEENDLDTLTNNNINLKVFDKELDLVIHILENYGYDIK
                     TDYKKKFEITDNADTIKPINLNFSDFKDFSKNLNWVINGLVENFIQENSNGLIEDEDE
                     EY"
     gene            complement(5181..5306)
                     /locus_tag="ACOIAF_002570"
     CDS             complement(5181..5306)
                     /locus_tag="ACOIAF_002570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669574.1"
                     /translation="MNKSYMLKYTYKSPTNLFHGDKKYTLTYTKRVMSNLVYDMG"
     gene            complement(5363..6133)
                     /locus_tag="ACOIAF_002571"
     CDS             complement(5363..6133)
                     /locus_tag="ACOIAF_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGZ2669575.1"
                     /translation="MIILQIGKNLKKIRKQKELTQIQLAEISGISRNALINYENDKRI
                     PSIDTLSKLAKALKIEKTIFYFDIENEVMTNEDVLYLLQSLFPEEYYSFDEFLNAISN
                     LMEDDIRITSSLLLGAWSDKDQYYKLGEKLELNENQVYNWILSDCISNTELDFSLHPD
                     DIKEIIKNDVLTKQSIDELLDIGLSEESIETIYDYFSDSTDNGSNIRLKKLISQKENI
                     TIKNNDYFKLLQENNELKEKNEKLENTLKEIKKLLTKI"
     gene            6271..6471
                     /locus_tag="ACOIAF_002572"
     CDS             6271..6471
                     /locus_tag="ACOIAF_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGZ2669576.1"
                     /translation="MGINLKIFRIKKGLKQQELADRVGISRYYLSNLETGKANNPSND
                     LMIKLSRALDTTVEELFFSKED"
     gene            6475..6789
                     /locus_tag="ACOIAF_002573"
     CDS             6475..6789
                     /locus_tag="ACOIAF_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669577.1"
                     /translation="MKENNNLEVLKTLQATLNIIVKNEEEAQEKKQGYKIIENAKRNK
                     AELITCKEASKLYPIGESKFRQLCHSKNKGFPCIWINNRVYIIKNKLDDWFIENANGI
                     KL"
     gene            6811..7554
                     /locus_tag="ACOIAF_002574"
     CDS             6811..7554
                     /locus_tag="ACOIAF_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669578.1"
                     /translation="MSNKGWIKLYRELLDKSIWKDIKPERKVILITLLLMASHKENHV
                     FIDNKKIKIKFGQFISSYSKIAERCGKGVSYQMVRSCLLVFKNAGFLTYETTNKYIVI
                     TIENWELYQENIENQQPNQQTINNQSTTIKNIKNENNIIYSEIISKFNSTCIELSKVK
                     KLTDTRKRRINSRIKEYDKETVLEVINIVSKTSFLNGNNNTGWKANFDWIMNSSNFTK
                     ILEGNYNSKEIVINKETGIYNPDDSQLKW"
     gene            complement(8260..10173)
                     /gene="drmB"
                     /locus_tag="ACOIAF_002575"
     CDS             complement(8260..10173)
                     /gene="drmB"
                     /locus_tag="ACOIAF_002575"
                     /inference="COORDINATES: protein motif:HMM:NF038324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DrmB family protein"
                     /protein_id="HGZ2669579.1"
                     /translation="MSIKLDDKTHNLRINQATSVFGPGAMLDFIDQTLMTAHPNYWTN
                     TIKIQDDRLQATLRVNELRTPPTIDDRSSVPFVRFPRWYFCPKCRKFMPIEDWEKLYK
                     NAHKDKPMKIPICLTCSTKKSTKQLVPTGIITICEHGHIDDFPWVKWVHFRNKSGRKE
                     ICENPQILMKTGKSNLGLEGIELECSCGAKANMKGAFMRLKSSKSNDYGVPSEYKEMF
                     KCSGNSPWYNKKVNKCTKYSFASQRGASNVYYPKIESSIIIPPYSDTLNSKVLSSKGY
                     DTFKKFLDRAIKKNKLERFMDEDFEDFASDIAKETGFDTDKVSIVLKDLLPFEDSSPS
                     TTTRNQYREEEYEALIGNIDAKSKKTNDFTIEEQNLSDYDIPNFSKIVLVKRLREVRA
                     LTGFSRVNPPDNNIFGEEDIDDSCNDTKVISLRSRDCKKSFYPASEVRGEGIFIEINN
                     DMVKSWISSNPKIQDNASILNSRYNKDINVNDKAYRKITPKFLLLHTLAHLIIRELSF
                     VCGYATSSLRERIYCNNSENEKVMSGILVYTASGDSEGTLGGLVRQGMHDSLPSILNS
                     ALNRALWCSNDPICIDSTGQGRNSLNLGACHSCTLLPETSCEEYNLLLDRSMLIGDMD
                     NRDIGFFKNLILI"
     gene            complement(10175..13702)
                     /locus_tag="ACOIAF_002576"
     CDS             complement(10175..13702)
                     /locus_tag="ACOIAF_002576"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HGZ2669580.1"
                     /translation="MNCSELRDKTLDLVRKELLGPGSEDLCENIKYEIITDSPIERYS
                     TGILYPLNNNIDVSNTSEYDGEDNVFTSDDTQNELDRAISMSNETLPSSMGMTFFLKK
                     DAEVINFRVNYATYRNALYTDCHVKYTGDDFYSSNTINEYVELRGSFLYLKQKLTSEK
                     VYNLLKKHDLLDNAEAKFVLYKLANQCPDDENKTGGYVRVPHENLIVSIDMDKNYIKK
                     PISKDLDIVLSKRIYSNDFVSITCMLVNSQSGPKKYNNTIFQPELMVLAEDNNDLEFI
                     DIFTAGSVNTFKKLDSEEISLDLLYRNKKTFATGHGVAIDQDIQNDALIKLCTEFIPH
                     FEVPKLDFNVDELSDVSRELLSMKNLSDISDLSKDKKIYFLYKFVDAYDIWIDDLEED
                     SKDLSDVFKDVAYEHIKNCRDTKSRMLEGINLIKSNDEVYRAFSLMNRAILMQRCHSI
                     HITDRLPGSNNLDYIDYSNDNSAWRPFQLAYILTCLSSIENPDSKHRDLVDLLWIPTG
                     GGKTEAYLGLSAFTIFLRRIRNKENGNGTAIIMRYTLRLLAAQQFIRASILICACEII
                     RKENKSELGKKEISIGLWIGSDQTPNFNDEAEEMLNNLTGNNIKFRSSINSEYELDNL
                     KSKYNKFQLLKCPWCGTKLVKEWDKTSKKAVGDWGYRIKSRPKKFYLCCPEEDCSFED
                     MLPIQVVDEEIYNNPPTLLFGTVDKFAMITWTTEVASLFALDDDNSNLSPELIIQDEL
                     HLISGPLGTMVGLYETALDAMCSYKGIKPKIIASTATIRKAQNQVKNLYNRNVAQFPP
                     PGLTSSNSFFVKEMPLSKKRGRLYVGVIPSGKTQVTVEVRLMSALLQRLKMMENVELN
                     LLDKYWTLVSYFNSIRELGKCSTLLNDDIKDNMERLCKRVSAHPYQSLRNLNEQRELT
                     SRLTSTKINTTLDELSNTLTSNNLTVDTLLASNMISVGVDVDRLNLMVVVGQPKLTSE
                     YIQASSRVGRSNEGLIFTLYNPTKTRDRSHYEQFFSYHQSFYKFVEPTSITPFSEPAR
                     ERGMHAVLISMIRHILGLQANDLASRFSKNMDGVEDLKNFIIDRSIDIFRENNQNADD
                     NIVNEFKSEISSELDIIMDLWHDKAQISDKDNLLHYYKCPSKFDNKIYNNLLIPYDKD
                     VITNEKQTLNSMRNVDKQCTVEIIELEEN"
     gene            complement(13720..16338)
                     /locus_tag="ACOIAF_002577"
     CDS             complement(13720..16338)
                     /locus_tag="ACOIAF_002577"
                     /inference="COORDINATES: protein motif:HMM:NF038316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DrmE family protein"
                     /protein_id="HGZ2669581.1"
                     /translation="MLTTNIHYILDNDICEIYFNKQRLTNSKYYQDNINVLNYIINEK
                     NNNLIINYNDKYIYAIYLLNIAINSYLNNTLNPNNHILTKLDKGMPLLYKGQIVIFKN
                     INNTHITLGHKNKTDTTLIPIDSAYMLITYNGHLEVNSMGKTPASKTFQTKNVLSSLG
                     FSDLKNMTGVINDSTLIILPCKDDISDLVSNIKIKDINNTYKFTELFPCSYISSTGAE
                     TDYPGNHAKQIPLLKFTTNISSAFEIIKSDKSIKNVFIIGDYPIYKHINDFERILNRK
                     RIESVNIVTSNSNISNILDLPNIDNLNIYSWSKDVLLTYSQDFENTSTFQKLWLDKLI
                     NKNICTTSVDSNISDLIHQARKSLYYISKYDFDISIKNSLMMCSYSLLKILENTPFNL
                     DFLEETIKLLNLNIATPSAKLENIKYLISSTSPDLELSALFDDVIVKFERIINELSDN
                     NTKFNTLNKLVSSYKYKTFTPNTPVILLQKQYEAIILKNLLKSRNINLQVISAEKFKD
                     SKTIKNLIMFGFYDLKYLDILNSDLIINITLYLYNSELSKYNYYMNQINSARNKIEKN
                     NKIYNLLDIKPFKPTYETNKLEAYSTDSPISDDDSMDEYISNYKFDINLNEIILSLSI
                     NQGIYNSKKEHVTSFMISDNNEYIFCTKKAIAYSIDWDKKSVRKKKLSEAKVNDWFLF
                     VDDYLNEEGELVINVIQKLLELNLLPSHYLDDYKLTNSWKSELKNYISYSKYNYNDVS
                     NSLAVLGEKINPITIKNWVTNKRLIGPIYLKTFEKIFKVIDSKLDHTEVYDATKSIRS
                     LHTKIKKSIDTFVSDNFFSSKSDIIKDEVSAVVLSMLGNTNDYVTPVQIREIHSCDKY
                     LSPHLVNKVLDLYSVY"
     gene            16650..>17059
                     /locus_tag="ACOIAF_002578"
     CDS             16650..>17059
                     /locus_tag="ACOIAF_002578"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HGZ2669582.1"
                     /translation="MYNVIDLFAGAGGLSLGFEMTKKFNMVAFVEKNDNAAKTYLENH
                     PSVKRYCDIKRLDFQDILNSVDKIDVVIGGPPCQGFSNANRQKRKIINGNNELVKLYV
                     DAIDKLKPNVFVMENVKTISSNKHSFYLTKKDKN"
BASE COUNT         6115 a         2280 c         2291 g         6373 t
ORIGIN      
        1 tataatatgt aatatttttg ttttgaaatt tataaattag tattactggt tagagttagt
       61 ttagtattaa gtatattcga agtagttttt tagctatttt catacattta tattacttaa
      121 ttatatatag cctaactaac ttaatatatc agtgaaatat tgactaaaat gcctaaatat
      181 gttatcatac aagttagtca aataatttgg caacattaaa tatttagtaa atttctaaca
      241 tttatggaac aaatttctag caaagaaggt gagatttata tggctggtaa aaaaatatta
      301 gcaacaaaca aaagagcaag acatgaatat tttatagaag aaacttatga atgtggaatt
      361 gaattaaaag gaacagaagt aaaatcaata agacaaggca aagttaacct aagtgatgga
      421 tttgcatctg tagataatag cgaagtattt ttaaaacaag ttcatataag tccatatgag
      481 caaggaaata tattcaatgt tgattcttta agagttagaa aactattact tcataagcat
      541 gaaataagaa agcttattgg agcaactact ataaaggggt attcacttat accacttagt
      601 ttgtatctaa aaaacggtaa agttaaagta gagattgcct tagcaaaagg taaaaaacta
      661 tatgataaac gtcaagatat ggctaagaaa gatgcacaaa gacgtataga aagagaaatg
      721 acaggtaagt attaaactag ataaaactgt attttttaga ataaaattct ttattatcta
      781 gaacataaca ttgattttta ttcttaaaag tattatatta tatatacatt aaaaatttaa
      841 taaaaatggt gataaagttt gagaaaagtc tactggacta taaactactc aagggggcgt
      901 aaaggtttcg acgtgggttt ggaacttgag gctgcatgtc gtgttactct gggtcacgta
      961 aaaactgggg aactttaaaa taaacgcaga cgataatttc gcgatagcag cctagttgct
     1021 gctcgtccct cttagtcctc ctgccgacta agactggacg tcattatgca gggaactact
     1081 ttaggggtgt ctcgacttga agtagattaa ttgggactgg ctaagcatag agcctgccac
     1141 ttggcgctat gtgaggtgag atattagtga agttggctaa gcatgtagat gcagcaagga
     1201 aaagatttgc ggacaggggt tcgactcccc tcgtctccac caattttgaa aaaagaatga
     1261 tgaaatactt ggaaatacta gtctttgttg actagtattt tttattttgg agaagaaatc
     1321 caccaaccgt accccaacca cagaaaatga ggtttttttg ggggtatatt ggggtgcatt
     1381 taggctgtat aaaaagtttt tatttgttta ttattttatt tgtttattat tttatttgtt
     1441 tattatgata ataggtaaca tagaatttta atatctactt tatgatataa tatttttata
     1501 aatgtaagac agtttaagtt gttaattagt aaaaatatat attatataag cttgatagga
     1561 gttgaggttt gtaatggatg aaagaaaact agaattttct attttttgca ttgaagcttt
     1621 agcagaaaaa ttgaatatag attcagaagg agtttataaa cttcttaaac atgatactga
     1681 tattttagat aactatataa taccttgcta tgaagcacta cattctcaaa gtaaaggtta
     1741 catagtggaa gatttaatgc aagtacttaa agaaaagggt gtgatataat gattgtatat
     1801 catggttcat attgtgtggt aaagacgcct agtattacct tttcaagaga ttctttagat
     1861 tttggaaaag gattctatgt aaccagtata aagacacaag caattaactg gacagagcgt
     1921 tttaaaaaaa gaggtaaaaa agcatattta aatagttatt ctttagatat tgataaatta
     1981 aaagaaaact gcaaggtaaa aatcttcgat agttataatt tagaatggct tgattttata
     2041 atagaatgta gaaatatgag tgatatatat atgaaatatg acattataat aggtggtatt
     2101 gctgatgata agatttacaa tacaatagaa ctgtatcaag ataatcttat tgaaaaagat
     2161 gaagctttaa aaagattaaa atactataaa ccaaatgaac aaatatgtat aaccaatcaa
     2221 aatgtaatag ataaatattt aaagtttagg tatgaagagg aggtataatg attatggaag
     2281 caaataaaac attattacaa atactatata aaaaaatcat attagaattt tccaaacaaa
     2341 caggtaagag cttggaagaa agtatggatt atttatatac ttctgaaaca tataaactta
     2401 taagtgaggg tgtatcagat atgcactgta agggtcatat atacttggct caagaattaa
     2461 tgttagaaaa tggattaatg taccataagg gctatccaag ataaataatt aaaactagat
     2521 acgaaataag gatacaatat aatataaaat taatttgtat ccttattttg attttgtaat
     2581 taactatatg aaatcacaat atttatatgt atttgatttt tacaatgaaa atattttatc
     2641 caacttttct acagcttcaa ctttcttatc ttccataact tgagtatata tattcaaagt
     2701 tattgatata tcataatgac ccattaaaac ttgtacagtt ttaggaggaa catcattctc
     2761 aaaaagtcta gtagcataag ttttcctaag accatgaaac tttataggct ctatatttaa
     2821 gctatttaat atagacttta aattccttaa aggtctttta tcatctatag ggatgcctaa
     2881 ttcattgcaa aatacataat tattatttat atataattct ccagccttta atatatctgt
     2941 attttgattt actttatgct ctttcaattt atttaaaaca tcttttggaa taggtacagt
     3001 tctgtaagaa tttgatgtct ttggcccttg ctctaatact ttaagctctc tatttccagt
     3061 tttatctata aaatatgttc tttgaagagt tcttttaact gttaaattag actttttaaa
     3121 atcaatatca aaccatttaa gtcctaataa ttctcctaat ctaagtccag taccaagagc
     3181 aagtaaaaat aatacctcta atttatgccc atctatagct tttataaaac tcttttgttg
     3241 ttcaagagtc aatatttcaa gtttgttttc ttttttctca actggtatat ttaccatagt
     3301 acaatagtta cgttgtataa gaccttgttt ttcagcttct gataagcagg ttttcagttt
     3361 agtgtttatt tgccttatag taggtatact tttattggaa tcaagcaatc tattgtaata
     3421 tttttgtatg tgagatactc ttaagtactt taatttaaca ttgcctaaat cagtatcttt
     3481 aatataatta cgatagatgc tttcatatct ttcaaatgac ttgggtttta aatcttttat
     3541 tctatattca aataaccaat tgtaaaacca atgctctaaa gttattttat catctgaaat
     3601 atcagagctt aaataatatt gttttttata agcatctaat ttatctttaa cttccttttg
     3661 agtcttacca taaaattctt ttctcagtgg tttaccatct tcattatagc caatcattat
     3721 acgggaacgc caaccatctt tatattttcc gatagagcct tctccattag ctcttttagt
     3781 tgccattttt aaacagctcc ttatatgatt tatagttata tccatcaaag aatgtaatac
     3841 atttctctaa taagaatttt ataacttcat cagcttctag tgtttcatag aagtttagca
     3901 ttaatgtgtt gtactcttgt ttatgtttag ctggtattga tagaactcct atatcattat
     3961 ttataagtat taggtttgag gtaagtctag cagttctttt gttgccatca tagaagaatt
     4021 ggcaatagga caaccataaa tttattatta tagccttttc taatgggtta gttattttat
     4081 ttatgatatt tatgtcattt acaaataaat catttaatag agtggcttct atgcattcat
     4141 attgagtagt accagctata cctatattac catttctgaa atctccaacg attagagctt
     4201 catcttttgc aactatttta tgtatagagc aaattgtatc tttatttaaa gataagtttt
     4261 ctttgttact taattttatt acataatccc atgatgattt tatatttagt atttgatttt
     4321 ggtcacttaa tttatgtccg cctacggtta taccatcaag taatgtttgt acttctggga
     4381 atgtgaaggg gttattttct aggttaccca tatcatatac aaattcactt atcattcttc
     4441 taatgtatgt taaagtatat tttttatctc catgaggtat attagtaggg aatttatgtg
     4501 tgtattttgg tatatatgat ttattcatga ttttagctcc tttcatatct aatttttatt
     4561 tattaactat gaatgtataa aagttaatag gtaaactcat attttgaaaa tgtgcaatac
     4621 taatattctt catcctcatc ttcaataaga ccattagagt tttcttgtat aaaattttca
     4681 acaagaccat ttatcaccca atttagattt ttggagaagt ctttgaagtc agagaagttt
     4741 aggttaattg gttttattgt atctgcatta tctgtaattt caaacttttt tttatagtct
     4801 gtttttatat cataaccata attttctaat atgtgaatta ctaaatccaa ctctttatca
     4861 aatactttta aattaatatt attatttgtt aaagtatcta aatcattttc ttcaacaatg
     4921 agtttattta tagcaattga taaagcagta gctatttttc ttatcatatc taaactaggc
     4981 attctttgat tttgctcata tttagctata gcatgaactg aacaaccaat cttgttggct
     5041 agctcatccc ttgacatatt tatcgatttt ctatattgtt taatcttttc tccaattgta
     5101 ttattcatca taatttcacc ttaaatatat catcaaacaa tgattgtact tctaaacatg
     5161 taaaggagtt attttctaga ttatcccata tcatatacta aattactcat aactctctta
     5221 gtgtatgtta gagtatattt tttatctcca tgaaataaat tagtagggga tttatatgtg
     5281 tattttagca tatatgattt attcataatt gtaactccat tcatagtttt ttatcattgc
     5341 tttagatagt attgatatta ttttatattt tagttagtag tttttttatt tctttaagtg
     5401 tattttcaag cttttcattt ttttccttta gttcgttatt ttcttgtaat aatttaaagt
     5461 aatcattatt tttaatagta atattttctt tttgagatat taattttttt agtcgaatat
     5521 tacttccatt atctgttgag tcactaaagt aatcatagat tgtttctata ctttcttctg
     5581 atagaccaat atctaataat tcatcaattg attgttttgt aagaacatca tttttaataa
     5641 tttcttttat gtcatcagga tgtaatgaaa aatctaattc agtattagat atacaatctg
     5701 aaagtatcca attataaact tgattttcat ttagttctaa tttttcacca agtttataat
     5761 attggtcttt atcagaccag gcacctagca ataaacttga ggtaattcta atatcatctt
     5821 ccataagatt tgaaatagca tttaaaaatt catcaaaaga atagtattct tctgggaata
     5881 aagactgtaa aaggtataaa acatcttcat tagtcattac ttcattttca atatcaaaat
     5941 aaaagatagt cttttcaatt ttcaaagctt tagcgagttt tgataaggta tctatactag
     6001 gtattctttt atcattttca taatttatta gagcatttct tgagatgcca ctaatttcag
     6061 caagttgaat ctgcgttagc tctttttgtt ttcttatctt ttttaagttt tttccaattt
     6121 gcaatattat cacctcgtat aaagtatatc ccaaaagtac acaaatgaga acagaaaaat
     6181 aatatattct tattaacagt acacatatat cgacattata attaaaatgt aaagtttacg
     6241 aatgtaaact agaaaaaagg aggaaaaagt atgggaatca atttaaaaat atttaggatt
     6301 aaaaaaggtc taaagcaaca agaactggca gatagagttg gtatatcaag atattattta
     6361 tcaaacttgg aaacaggtaa ggctaataat ccaagcaatg atttgatgat taaactatct
     6421 agagctttag atacaacagt agaagaatta ttttttagta aggaggatta aaatatgaaa
     6481 gagaataata atttagaggt tttaaagaca ttacaagcca ctttaaacat tattgtaaag
     6541 aatgaagaag aagcacaaga aaaaaagcaa ggatataaaa ttattgaaaa tgcgaaaaga
     6601 aataaagctg agcttataac atgtaaagaa gcatctaagc tttatcctat aggagaatca
     6661 aaatttaggc agttatgtca ttctaaaaac aaaggattcc cttgtatatg gataaataat
     6721 cgagtataca taataaaaaa taaattagat gattggttta ttgaaaatgc aaatggtatc
     6781 aagttatagt cttaaagtag gtgtttgcaa ttgagcaata aagggtggat aaagctttat
     6841 agggagttgt tggataaatc aatttggaaa gatattaaac cagaaagaaa ggtaatatta
     6901 ataactctat tacttatggc tagtcataaa gaaaatcatg tttttataga taataaaaag
     6961 ataaaaataa agtttggaca atttatttct tcatatagca aaatagctga aagatgtggt
     7021 aaaggagtta gttatcaaat ggttagaagt tgtttgttgg tttttaaaaa tgctggtttt
     7081 ctaacatacg aaacaacaaa caagtatata gtaataacta ttgaaaactg ggagctttat
     7141 caagagaata tagaaaatca acaacctaat caacagacta ttaacaatca gtcaacaaca
     7201 atcaagaata taaagaatga aaataatatt atatatagtg aaatcataag caaatttaac
     7261 tcaacttgta ttgaactttc aaaggtcaag aaactaacag atacaagaaa gaggagaata
     7321 aattcaagaa taaaagaata tgataaagaa actgtattag aggttataaa tatagttagt
     7381 aaaacaagtt ttctgaatgg aaataataat acaggatgga aagcaaattt tgattggata
     7441 atgaattcaa gtaattttac taagatacta gagggaaatt ataatagcaa ggaaattgtc
     7501 ataaataaag agacaggaat atataatcca gatgactcac aattaaagtg gtaaggaggt
     7561 tatgttataa ataattcaaa caacttaatt aaaaatacag aagcagagca atcaattaaa
     7621 ttacactaag ttagttataa atctttcata tatttgtttt ttaagtatct ctaatatcgt
     7681 ttaaattaca ctaagttagt tgcagatata ccagaaaata tatataaaat tatgcgtaaa
     7741 ctatattgtt atgagtacaa ataatattta cctattcatt aaaaaactta actaagaatt
     7801 taatttatat tattgaagta tcaataattt tggaaagtag aaaattatac cccaataata
     7861 ccccaactac aaaaatagac aataaaaaat aaccagaaaa attgcttcaa catcatgacc
     7921 aaatagccat atacctatac ttataaaaac caacaaaaca atcaaataac cacttataaa
     7981 ttctcctcgg tctccaccat tggaatccac gaaaacatat tgatttaata gttaatatgt
     8041 tttttatttt gaaaaatcat atataatata aattaatagt ttatttaaaa atttcagtat
     8101 gagcacaaaa ttatataatt attgggaatt agttataata agtaatttct agtaagaatg
     8161 atttgcaaaa aataataatt taaatacaaa aaactaaagg atagaagtca taaaattaac
     8221 ttttaacctt tagttatata aataaaaaat tagttattac taaattaaaa ttagattttt
     8281 aaagaaccca atatctctat tatccatatc accaattaac atgcttctat caagtaataa
     8341 attatactcc tcacagctag tttcaggcaa taaagtacat gaatggcatg ctcctagatt
     8401 taaagaattt ctaccttgac cagtactatc aatacatatt gggtcattag aacaccaaag
     8461 agcacgatta agagcactat ttaatataga tggtaatgaa tcatgcatac cttgcctaac
     8521 taatcctcct aaagtacctt cagagtcacc acttgcagtg taaactaaaa ttccagacat
     8581 aactttttca ttttcggaat tattacaata tatacgttcc ctaagtgaag aggtagcata
     8641 tccacaaaca aaactaagtt ctcttataat taaatgagct aaagtatgta gtaataagaa
     8701 tttaggtgta atttttctat aagctttgtc attaacatta atatctttat tatatctaga
     8761 atttaaaata gatgcattat cctgtatttt aggattagat gaaatccagc tttttaccat
     8821 atcattattt atctctatga aaataccttc acctctaacc tcagatgcag gataaaaaga
     8881 ttttttacaa tctctagatc ttaaacttat aaccttagta tcattacagc tatcatctat
     8941 atcttcttca ccaaatatat tattatcagg tggattgact ctactaaacc cagttaatgc
     9001 ccttacttct ctaagtcttt ttactaacac tattttactg aagtttggaa tatcataatc
     9061 agatagattt tgttcttcta ttgtaaagtc attagttttt ttagatttag catctatatt
     9121 tccaattaaa gcctcatatt cttcttctct atattgattt ctagttgttg ttgatggtga
     9181 agaatcttca aaaggcaata aatctttcaa aactatggat actttatcag tatcaaaacc
     9241 tgtttcttta gcaatatcac tggcaaagtc ctcaaaatcc tcatccataa agcgttctaa
     9301 cttattcttt ttaatagcac gatctaagaa tttcttaaat gtatcataac ctttactaga
     9361 tagcacttta gaattaagag tatcagagta tggtggtata ataattgaac tttctatctt
     9421 aggataatat acattagagg cacctctttg agaagcaaat gaatatttag tgcatttatt
     9481 tactttttta ttataccaag gcgaattacc agagcattta aacatttcct tatattcact
     9541 aggtacacca taatcattag atttagaaga ttttaaacgc ataaatgcgc ccttcatatt
     9601 agctttagct ccacagctac attcaagttc tataccttct aatcctaggt tactttttcc
     9661 agtcttcatt aatatttgag gattttcaca aatttctttt cttccagatt tatttctaaa
     9721 atgaacccat tttacccatg gaaaatcatc aatatgacca tgttcacaaa tagtaattat
     9781 accagtaggt actaattgtt tagtagactt ttttgtgcta caagtaaggc aaataggtat
     9841 tttcataggt ttatctttat gagcattttt ataaagtttc tcccaatctt ctataggcat
     9901 aaattttcta cattttggac aaaagtacca tctaggaaac ctaacaaaag gaacagaaga
     9961 tctatcatct atagttggag gtgttcttag ttcattaacc ctcaatgtag cctgaagcct
    10021 atcatcttgt attttaattg tattagtcca ataatttgga tgagcagtca ttaatgtttg
    10081 atcaataaaa tctagcatag ctcctggtcc aaaaacactt gtagcttgat ttattcttaa
    10141 attatgagtt ttatcatcta attttataga catattaatt ttcctccaat tctataattt
    10201 caacagtaca ttgtttatca acatttctca tagaatttaa tgtttgtttt tcatttgtaa
    10261 taacatcctt atcataagga attaaaagat tattatatat tttattatca aatttactag
    10321 ggcacttata ataatgcaat aaattatctt tatcagatat ttgagcttta tcatgccata
    10381 aatccattat aatatctaat tcagatgaaa tctcagattt aaattcattt actatattat
    10441 catcagcatt ttgattattt tctctaaata tatcgataga tctatctatt atgaagtttt
    10501 ttaaatcttc tacaccatcc atattttttg aaaatcgact tgcaagatca ttagcttgaa
    10561 gtcctaaaat atgtctaatc atacttatta atactgcatg catgcctctt tctctagctg
    10621 gttcagagaa aggtgtaata ctagtaggtt caacaaattt atagaatgat tgatgataac
    10681 taaagaactg ttcataatga gatctatctc ttgtttttgt aggattatat aatgtaaata
    10741 taagtccttc attacttcta ccaactctac tgctagcttg tatgtattca gatgttaact
    10801 taggctgacc aactactacc attaaattta atctatcaac atcaacacca actgaaatca
    10861 tattactagc taatagagta tcgacagtta aattattaga agtaagagta ttagatagtt
    10921 catctaaagt tgtatttatc ttagtagaag taagtctact agtaagctct ctttgttcat
    10981 ttaggttcct taatgattga taagggtggg ctgagactct tttacataat ctttccatgt
    11041 tgtcttttat atcgtcattt aaaagtgtgc tacacttacc taattctctg atactattaa
    11101 aataagatac tagtgtccaa tatttatcca ataaattcaa ctctacattc tccatcattt
    11161 taagtctttg gagtaatgct gacataagtc taacctctac agtaacttga gtttttccag
    11221 atggaataac acctacatat agtcggccac gttttttact taaaggcatc tctttaacaa
    11281 agaaagagtt actagatgtt aatcctggag gtggaaactg agctacatta cgattatata
    11341 gatttttaac ttgattttgt gcttttctta tagtagcagt agaagctata atcttaggtt
    11401 ttataccctt atagctacac atagcatcaa gtgcagtttc atataaacct accatagtac
    11461 ctaaaggtcc agaaataaga tgaagctcat cttgaattat taactcagga gataagttgc
    11521 tattatcatc atctaaagca aaaagactag ctacttctgt agtccaagtt atcattgcaa
    11581 atttatcaac tgtaccaaat aaaagtgttg gagggttatt ataaatctcc tcatctacaa
    11641 cttgtatagg taacatatct tcaaatgaac aatcttcttc aggacagcat aagtagaatt
    11701 tcttaggtct agatttaatt ctatagcccc aatcaccaac agctttttta gaagttttat
    11761 cccattcttt aacaagtttt gttccacacc aaggacattt tagtaattgg aatttattat
    11821 attttgattt taaattatct aactcatatt cactatttat actagatcta aacttaatgt
    11881 tattacctgt taagttatta agcatttctt ctgcctcatc attaaagttt ggagtttgat
    11941 cactcccaat ccacaaacca atagaaatct cttttttccc aagttcagac ttattttctt
    12001 ttctaataat ttcacaggca caaattaata tacttgctct tatgaattgt tgtgcagcca
    12061 atagccttag tgtatatctc attatgatag ctgtaccgtt accattttct ttgtttctta
    12121 ttcttcttaa gaagatagta aaagcagata gacctaagta ggcctccgtt ttacctccac
    12181 cagtagggat ccaaagaagg tcaactaaat ctctatgttt agagtcagga ttttctatcg
    12241 aagataaaca tgtaagtata taagcaagct ggaatggtct ccaagcagaa ttatcatttg
    12301 agtaatctat atagtcaagg ttattagatc cagggagtct atccgtaata tgtatagaat
    12361 gacatctttg cataagtata gctctattca ttagagaaaa tgctctgtat acttcatcat
    12421 tagacttaat taaatttata ccttctagca ttctagactt agtatctctg cagtttttta
    12481 tatgctcata tgctacatct ttaaaaacat cactaagatc ttttgaatct tcttctagat
    12541 catctatcca aatatcataa gcatcaacaa acttataaag aaaatatatt ttcttatctt
    12601 tactaagatc agagatatct gataaatttt tcatagacag taattctcta gaaacatcac
    12661 ttaattcatc tacattaaag tccaatttag gtacttcaaa atgaggaata aactcagtac
    12721 aaagttttat taaagcatca ttttgaatat cttgatctat agctacacca tgaccagtag
    12781 caaaagtttt tttatttcgg taaagtaagt ctaaagatat ttcctcagaa tctaactttt
    12841 taaaagtatt tactgatcca gctgtaaaaa tatctataaa ctctaaatca ttgttatctt
    12901 cagctaatac cattagttca ggctggaaaa tagtattatt atatttctta ggcccagatt
    12961 gactattaac cagcatacaa gtaatactaa caaagtcatt tgaataaatt cttttagata
    13021 aaactatatc taaatcttta gatataggtt ttttaatata gtttttatcc atatctattg
    13081 atactattaa gttttcatgt ggaacacgaa cataaccacc agtcttattc tcatcatcag
    13141 ggcattggtt agctaacttg tataaaacaa atttagcttc agcattatca agtaaatcat
    13201 gcttttttag taaattataa accttctcag atgttagttt ttgcttaaga tataggaatg
    13261 atcccctaag ttctacatat tcattaattg tattgctaga atagaagtcg tcaccagtat
    13321 atttaacatg acagtctgta tataaagcat ttctataagt agcataattt accctgaaat
    13381 ttattacttc tgcatctttt tttaggaaaa aagtcatacc catagaagaa ggtaaagttt
    13441 cattagacat acttatagct ctatctagtt cattttgtgt atcatctgat gtaaatacat
    13501 tatcttcacc atcatattca gatgtattac ttacatcaat attgttatta agtggatata
    13561 aaatacctgt tgaataacgc tctattggac tatctgttat tatttcatat tttatatttt
    13621 cacaaagatc ttccgaacca ggtccaagaa gttcttttct tacaagatct agggttttat
    13681 ctcttagttc actacaattc atatttacct cctatttatt taataaacac tatataagtc
    13741 taaaactttg ttaacaaggt ggggtgacag atacttatca caactgtgta tttctcgtat
    13801 ttgtacaggt gtaacataat catttgtatt cccaagcata cttaaaacaa ctgcagacac
    13861 ttcatcttta attatatctg atttactgct aaagaaatta tcagatacaa atgtatctat
    13921 agattttttt attttagtgt gcaaagagcg tatactttta gtagcatcat atacctcggt
    13981 atgatctaat ttagaatcta taactttaaa tatcttttcg aaagttttta aatatatagg
    14041 acctattaat cttttatttg ttacccagtt tttgattgtt ataggattaa tcttttcacc
    14101 taaaacagct aaactgtttg atacatcatt ataattatat ttactataag atatatagtt
    14161 tttaagttct gatttccatg aattagttaa tttataatca tcaagataat gagatggcaa
    14221 taaattaagt tctaatagct tttgtataac attaataact aattcaccct cttcatttaa
    14281 gtaatcatca acaaataaaa accagtcatt aactttagcc tctgaaagtt tctttttcct
    14341 aacacttttt ttatcccaat ctattgaata ggctattgcc ttttttgtac agaatatata
    14401 ctcattatta tcagaaatca tgaatgatgt aacatgttct tttttagagt tgtaaatccc
    14461 ttgatttata gatagagata atataatttc atttaaattt atatcaaatt tataattaga
    14521 tatatattca tccatagaat catcatcaga aataggacta tcggtagaat aagcttctaa
    14581 tttattggtt tcataagttg gtttaaaagg ttttatatca agtaagttat atattttatt
    14641 atttttttca attttatttc ttgcagaatt tatttgattc atatagtaat tatatttgga
    14701 taattcacta ttatataaat acagtgtaat atttataatc aaatcactat ttaaaatatc
    14761 taaatatttc aaatcataga atccaaacat aataagattt tttattgttt ttgaatcttt
    14821 aaatttttca gcagaaataa cttgtaaatt tatatttcta gattttaata aatttttaag
    14881 tataatagct tcatattgtt tttgtagcaa tataacaggt gtatttggtg taaatgtttt
    14941 gtatttatat gaagatacta atttatttag tgtattgaat ttggtattat tatcagataa
    15001 ttcatttata attctttcaa atttgactat aacatcatca aataatgcac ttaattctaa
    15061 atctgggcta gtacttgaaa ttaaatattt tatattttct agcttcgctg aaggagttgc
    15121 tatatttaaa tttaaaagct ttatagtttc ttctaaaaaa tctaggttaa aaggtgtatt
    15181 ttctaagatt ttaagaagag agtaactaca catcataagt gagtttttta tggaaatatc
    15241 aaaatcatac ttagatatgt aataaagtga ttttctagcc tgatgtatca agtcagagat
    15301 attagaatca acagaagtag tacatatatt tttatttata agtttatcta gccataattt
    15361 ctgaaaagta gaggtatttt caaaatcttg gctataggtt aacagaacat ctttagacca
    15421 agagtatata tttagattat ctatatttgg taaatctaat atattgctta tatttgaatt
    15481 tgaggttaca atatttacac tttctatcct ttttctattt aaaattcttt caaaatcatt
    15541 tatatgctta taaattggat aatcacctat tataaataca ttttttatag acttatcaga
    15601 tttaataatt tcaaatgcac tagaaatatt agttgtaaat ttaagtaaag gtatttgctt
    15661 agcgtgattt ccaggataat cagtttcggc accagttgat gaaatatatg aacatggaaa
    15721 tagttcagta aacttataag tattattaat atcttttatt ttaatattac taactaagtc
    15781 agatatatca tctttacaag gcaatataat cagtgtagaa tcatttatta ctccagtcat
    15841 attttttaag tctgaaaaac ctaaagatga tagaacattc tttgtttgaa aagtcttaga
    15901 tgctggagtt ttgcccatag aattgacttc taaatgacca ttataggtta taagcatata
    15961 agccgagtca ataggtataa gtgttgtatc cgttttattt ttatgtccta aagttatgtg
    16021 tgtattattt atatttttaa atataactat ttgacccttg tagagcaggg gcataccttt
    16081 atcgagtttt gtaagaatgt gattatttgg gtttaatgta ttatttaagt atgagtttat
    16141 agctatattc aacaaataaa tagcatatat gtatttgtca ttataattga taattaagtt
    16201 attatttttt tcatttataa tataatttaa tacattaata ttatcttgat aatacttaga
    16261 atttgttagc ctttgtttat taaaataaat ttcacatata tcattatcaa gaatataatg
    16321 gatatttgta gttaacattt aaatctcctt tagtagttac tattgtttat tagaatagtt
    16381 tttattctat attataatta tattactaaa aaataatata tggtatataa tataaaaaaa
    16441 gattgataaa taaataaaaa atatattatg ttatttattt gaatagatta tccagaagac
    16501 ataaataaga aaaataatgt gtaagtaaaa atattagaat ttattaaaag ctattacaag
    16561 tataagaaat ttgaaatgat tattcaaaaa ttgacatgtg aaaaaaaata tgatatgctg
    16621 caataaaagt taatgtgagg tatgtcagaa tgtataatgt tatagatctt tttgcaggtg
    16681 caggtggttt gagcttagga tttgaaatga ctaaaaaatt taatatggtg gcttttgtag
    16741 aaaaaaatga taacgcagca aaaacatacc ttgaaaatca tccgagtgtt aagcgttatt
    16801 gtgatattaa aagattagat tttcaagata tattaaacag tgtcgataag atagatgttg
    16861 ttataggagg accgccatgt caaggttttt ctaatgctaa tagacaaaaa agaaaaatta
    16921 taaatggaaa taatgaatta gttaagctat atgttgatgc tattgataaa ttaaagccaa
    16981 atgtatttgt aatggaaaat gtcaaaacta tatcttcaaa taagcattca ttttatttga
    17041 ctaaaaaaga taaaaatca
//
LOCUS       DBGJFF010000081        16698 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.082, whole genome shotgun sequence.
ACCESSION   DBGJFF010000081 DBGJFF010000000
VERSION     DBGJFF010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16698
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.082"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            161..862
                     /locus_tag="ACOIAF_002579"
     CDS             161..862
                     /locus_tag="ACOIAF_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4253 domain-containing protein"
                     /protein_id="HGZ2669583.1"
                     /translation="MSIEDLYDFECVPLNNEIDIDVLFEKMIEDGKEKEYTPIIVIDE
                     KVGLLRKNIDMITKEYGSLENYREYCLTKYNEIDVNQFFDFRKSEISELLKELIDLED
                     DFYDYKHFRPIMNIDIFHEYNKVYIAKIPTIKPYEVFAYIPIGGFNDCPRDEEHIAIA
                     KYWYEKHGIFPIAIGCDTVQYSVKNLTKDGKKFDDLCIELVFYCEDIIIQGYETLKAL
                     KDVLKRSTIVLFWWD"
     gene            1133..1951
                     /gene="larE"
                     /locus_tag="ACOIAF_002580"
     CDS             1133..1951
                     /gene="larE"
                     /locus_tag="ACOIAF_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HGZ2669584.1"
                     /translation="MEVNFVKEKEKLDKLKKMLLELGSVVVAYSGGVDSNFLLKVAKD
                     TLGENVIAVTIHAMMHSSREIEEAKQYTQNFGVKHIILNIENFDLKEFKENGVDRCYH
                     CKKYIFSKIKEVAKEHNIKYIVDGTNIDDLGDYRPGLKALSELGVISPLKDSSLKKEE
                     IRSLSKTLGLNTFNKPSFACLASRIPYGVEITDENLRIVEKSEEYLYNLGFSQFRVRM
                     HGDIARIEVGQEELGKFFENNNFNKVDTKLKIFGFKYVTLDMSGYKMGSMNLNV"
     gene            2006..3154
                     /locus_tag="ACOIAF_002581"
     CDS             2006..3154
                     /locus_tag="ACOIAF_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HGZ2669585.1"
                     /translation="MKNYTLISPCFFGMEKMLAREITNLGYEIIKTEDGRITYKTDEF
                     GIAKSNMWLRCAERVHLKIAEFEAKSFDELFENTKRINWSRYIPYGAQFPISKASSIK
                     SKLYSTPDVQAIVKKAIVESLKKSYLEDGLLKEDKEKYPIFVFIHKDKVTISIDTTGD
                     ALHKRGYREKANKAPIRETLAAGLIYLTPWKAGRVLVDPMCGSGTILIEAAMIGINMA
                     PGLNREFISEKWRTLDKKIWWDVRKDAFNKIDNESKFKIYGYDIDEESIDIARENAEI
                     AGVDEYIEFNVGDATQFKSEDEFGFIITNPPYGERLEDKDSVKQLYKELGYAFRKLKN
                     WSYYLITSYEDFEYEFGQKADKKRKLYNGMLKTNFFQYPGPKPPRNNK"
     gene            3290..4678
                     /locus_tag="ACOIAF_002582"
     CDS             3290..4678
                     /locus_tag="ACOIAF_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HGZ2669586.1"
                     /translation="MKVLKLITVVALMMLFVTGCNIKTESPEQLAKIPDYDDTKKVLY
                     DGIDQLLKPDSSMILPSNTKEVGKINKVDLNSDGIDELVVFEQKEDLSNNVSQVGFVT
                     LSYNGEKYVLGDHFLENGESIEYANFYDLDSDGYKEVILLVKSKDKTNMHIYKVVDNE
                     ITKIYDLDASWLQNKEDFNDMKVKIGYIDGDDKLDILMLHLNNKTNEMFASVANFDGK
                     MKIKDSVKFENVKNLSELYITLGNVASSVGNSSAAIKGIVLDIPILKDNNYITQILYM
                     KDGKINKAFSDYDKTITKSYYIPVDDIDKDKIIEIPIVAGSTGNNKNTYSSKSSATIS
                     WYRWNGKEGADSSLIFTSQIYYNYKYNFKLFIPNNLFDKILVEQEFVGEKAVFKFYYF
                     DYSDRKNLFNIVVESKNKLEDRKNTGTQNSIVLQESDEYTFLLVVNNANEMDKLDMTI
                     DALKEYFSLIYG"
     gene            4707..5405
                     /gene="walR"
                     /locus_tag="ACOIAF_002583"
     CDS             4707..5405
                     /gene="walR"
                     /locus_tag="ACOIAF_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism DNA-binding response
                     regulator WalR"
                     /protein_id="HGZ2669587.1"
                     /translation="MKEKILILEDEIGIRSFVSINLKREGYEIVEAGTGREAIEKMTT
                     EKDITIALLDVMLPDISGIEVCKFIRENFDQVGIIMLTAKAQEDDKIEGFISGADDYI
                     IKPFSIKELLVRVSALLRRVAKDDSSVKSSEIVSPPFILDIDKRKLFKNGKEIELTPT
                     EFSIVKYLISNAKQSLSRDQILDEVWGTNYLYDFKIVDVNIRRIRNKIEDDPSKPKYI
                     QTIWGYGYCFRKEE"
     gene            5408..6841
                     /gene="walK"
                     /locus_tag="ACOIAF_002584"
     CDS             5408..6841
                     /gene="walK"
                     /locus_tag="ACOIAF_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HGZ2669588.1"
                     /translation="MLHFEGLRKKVIKNYFIIIIIMVTLFEGLFMFYIQNYYYDSVKQ
                     LLESEIKYADEYNAITMETTSFEKKVKNIFDKQPLTKNSEFGISIIDKDKNIILDQYG
                     FKSKEKANYEDVNNALKDIKTKNLTPYTYRIPDTGEHVMSISLPLKVNNIIEGVVRYT
                     VSLDAIDNAILKQATWLILAGIFILIIAILISLKFAETLIKPLRELKKFANELAVGNY
                     NIKLEKMKIVDDEIGDLAQTFEHMAHEIDKSEKLKEEFISSVSHELRTPLTSIKGWSE
                     TLGYESITREELDLGLGIIQDETERLIKLVEELLDFSRLSSDRIKLHVDIVDVEGLIV
                     GVVNQLKVKAAEKDISLLFEFENEFIENIQGDKNRLRQVLINLIQNSFKFTSQGGYIK
                     VVASQDEEITTISVEDNGSGIEKQNLNKVLDKFFQEDYNKAGSGLGLAISNEIVKLHG
                     GRMKIESEKNVGTKITFNIKNKFAKQA"
     gene            6863..7600
                     /gene="truA"
                     /locus_tag="ACOIAF_002585"
     CDS             6863..7600
                     /gene="truA"
                     /locus_tag="ACOIAF_002585"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HGZ2669589.1"
                     /translation="MRNIKMIVAYDGSRYKGYQKLGDNNMTIQEKLENVLSKMTNETV
                     EIIGSGRTDMGAHARGQVVNFRTNCMDSLDKIQKYLYEYLPEDIVVKTVEEVDERFHS
                     RYNVKSKTYMYKIDNNKYHNPFIRKYATHVSKKLDLDRMRKASEYLVGEHDFTSFASS
                     KSKKKSNVREIYSINIKEDDNVIEIYVEGNGFLYNMVRIIVGALIDVGLKRRAPQDIK
                     YMLESKDRCQSSDTAPAKGLCLWKVTY"
     misc_binding    7711..7918
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8171..9370
                     /locus_tag="ACOIAF_002586"
     CDS             8171..9370
                     /locus_tag="ACOIAF_002586"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HGZ2669590.1"
                     /translation="MKEKVVLAYSGGLDTSIIIPWLKENYEDIDVIAVCGNVGQEDKM
                     EDVYEKALQSGASKAYVDDISEEFVTETIFKAVKAEAKYEGKYLLGTSLARPIIAKKL
                     VEVAHKEGAKYICHGCTGKGNDQVRFEATIAALDPTIKVIAPWRIWDIKSREDAIDYA
                     EKHNIKVTATKAKIYSVDANLWHVSTEGGDIEHLENEHKKDVYKQCVDPEDACDVAEY
                     VEVYFEKGVPKKVNGEELSPVALIHKLNELGCKHGIGVIDIVENRLVGMKSRGIYETP
                     GGTILYEAHNILESATLDKDTLHFKQMVSYKYGELIYNGLWYCKLRESIDAFMEQTQD
                     NVTGTVKVKLYKGNIKPAGIFTENALYDEGISSFGNSELYDHKDAEGFINLFTLPLKI
                     RAMKAGK"
     gene            9617..10663
                     /locus_tag="ACOIAF_002587"
     CDS             9617..10663
                     /locus_tag="ACOIAF_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtrH N-terminal domain-containing protein"
                     /protein_id="HGZ2669591.1"
                     /translation="MRKIIEIKHHSHDYECMWNGIEDLYINKTGECLPNDFFFLLSGF
                     GSFCYMKTNKADLKRMVALGDGRTKQMYKFLAPIVGFEYKHHEFKKYEQALKKAKSEI
                     DDGYPVILGALDMYYLPYYKKLYHKEHIPFHYILMVGYDDEGIYLYDCGRTELLHLSY
                     DKLRESMNCNSPGLSKENTICTIRMNTSKNKYNIAREAIAIKRDMFLNPPKGFLGYKG
                     FEKFIKELPQWNTELDKDDYDKILTNMVMFFGTVPTIPNAIKGIKEPDTVQFKGGFDK
                     MSRMLNSIGEEYRNNSFIQVADIFDKTAIIIEKISNIIIDYLTQTCDDTEQLPLLFSE
                     VLEHMKTGYLILDV"
     gene            10771..11934
                     /locus_tag="ACOIAF_002588"
     CDS             10771..11934
                     /locus_tag="ACOIAF_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HGZ2669592.1"
                     /translation="MRTVKQVSDLTGISVRALHYYDEIGLLKPNKITDAGYRLYDDES
                     IKTLQQILFFKEIDIPLREVKEIMSSQYFDKVEALKNQKKLLILKRKRLDELIELINQ
                     TLRGEGNIDFKEFDMSEYFDVLEEFKREHRNKVIKIYGSVEKYNEYIERVKSNEEKIA
                     KMAIKQYGTIEKFAKAIKTNFSSDILNLGEKFDRYKNDCLKEKHPKLKELYRKLVEDL
                     SRNPSSTDLQEIAKEITDISKKDYEIFSMDTGDDNWYYMVQNYLVNPMWIEEVDKKYG
                     SGAGKFIGQVLKTYLRDRKPKINTLYEKLVEDLSRDCSSREVQSIVEEIDNEMKRSNE
                     FYKIDNGERYFDYMSELYLQDSNYIKVTDKNYGDGASRFIGEAFKIYFDNNNC"
     gene            complement(12023..12403)
                     /locus_tag="ACOIAF_002589"
     CDS             complement(12023..12403)
                     /locus_tag="ACOIAF_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4363 family protein"
                     /protein_id="HGZ2669593.1"
                     /translation="MKSTTFVILWTILFVLFGFYVNNKLYDFTEGYKDNISVLEKSIE
                     NEDWEKAQKEADSISTSWNKEKNHWYKVLNHEYFDEIGLRFNILDKAIYTENKLKSLE
                     EVESIKTYLGNIIESVKFDVNYIF"
     gene            complement(12406..13065)
                     /locus_tag="ACOIAF_002590"
     CDS             complement(12406..13065)
                     /locus_tag="ACOIAF_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HGZ2669594.1"
                     /translation="MTVVLIRSIILYITVLIALRVMGKGEIAEMNCFDLVITLLIAEV
                     ASVPMENNNIPIINGVAAISGLVIMQTLISFLSLKSRKLSSFLSGKPSVLIDKGKIVY
                     KELKKERISIDELLEQLRIQGYFNLKDVQYAILETDGNLSVVPASSYNSTPPRAFNHL
                     PIPLILDGRIINKNLDIAQKDTNWLMGILKSNHIETFKDVLICVLDENDKIFIQNKKG
                     D"
     gene            complement(13171..14145)
                     /locus_tag="ACOIAF_002591"
     CDS             complement(13171..14145)
                     /locus_tag="ACOIAF_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HGZ2669595.1"
                     /translation="MKYKLFIDFEFNILDDNKNKPKEYNGAELISIGGVLVDNEFNTI
                     DNYYSLVKPKYNKILSNKCKNLTKLNQLDINNAPNLLYVMDDFYKWFCKFDDVTIYNW
                     GDFDITGLLTSFRVYKYTGKCLELFNMMIDIQPFISQHITYNNRILSKQISLLNMKKI
                     FSVEGDIKHNSLSDAVDLMNVCKCFFFNSPKNIDILEELYNKLPALKTPLYYIPYFED
                     ENFELKFDRTPEDIIIYLKQILTILNISKKEIYFKKRSVLVNNSKIINFKNLSSSFKL
                     IKLNEFEEYPDFILKLGDKKSFVESVIKINKSNRKSIKNLILKLSKKP"
     gene            complement(14185..14532)
                     /locus_tag="ACOIAF_002592"
     CDS             complement(14185..14532)
                     /locus_tag="ACOIAF_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669596.1"
                     /translation="MLNLKEIREEIIKKYIGDSLISELTEELKNILITDLQSNPFKSN
                     IELSIIPANIEIFKKIENILIDSKVNLKMGWERILINYILKDLFELNYEINKSCNEPL
                     MFRVIISIDSYDA"
     gene            complement(14750..15706)
                     /locus_tag="ACOIAF_002593"
     CDS             complement(14750..15706)
                     /locus_tag="ACOIAF_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HGZ2669597.1"
                     /translation="MKKILITGALGQIGSELTIKLRNEYGEQNVIASSRRVKEGNPVC
                     ESGIFEILDVTDKNRFFEIAKKYDVDTIIHLASLLSAVAESKPLEAWNLNMNGLINGL
                     EIAKELDCKFFTPSSIAAFGENSPKHMTPQDTLQRPNTMYGVTKVSGELLCDYYHSKF
                     GVDTRGVRFPGLISYVTPPGGGTTDYAVDIYYEALKNKRYKSYIAEGTKMDMMYMPDA
                     LQSIVDLIEAPADKLIHRNAFNITAMSFSPEEIADSIKKYIPDFVIEYDVDPVRQSIA
                     DSWPNSLDSSSAVKEWNFKFSYDLDKMTKDMLEKLSEKGIGK"
     gene            16149..16517
                     /locus_tag="ACOIAF_002594"
     CDS             16149..16517
                     /locus_tag="ACOIAF_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family (seleno)protein"
                     /protein_id="HGZ2669598.1"
                     /translation="MTRPSIYHSQGYTCAEALIKSYNEEHNTDIPISIGSGMGVGMNV
                     GSVCGAVNAAVVIIGYLNGRNSNLDENIARKYSRELLNRVRNKYSSEICVSLKKNKVT
                     CEEIINFTYDALNDMLKNQK"
BASE COUNT         6581 a         1751 c         2712 g         5654 t
ORIGIN      
        1 atacagtttt atatagaaaa aagaaatgaa tcttttcttt atataagtat attttgtaaa
       61 ttgcctctat atttatagtt taaataatag tggtattaaa aatctgacaa caaaaataaa
      121 actttcaaaa aattaaatct aaaaagaata ggagataaaa atgagtattg aagatttata
      181 tgattttgaa tgtgttcctt tgaacaatga gattgatatt gatgttttat ttgaaaagat
      241 gatagaagat ggaaaagaaa aagaatatac accaattata gtcattgatg aaaaagtagg
      301 gctattaaga aaaaatatag atatgattac taaagagtat ggttctcttg aaaactatag
      361 agagtattgt ttaacaaaat ataatgaaat tgatgtaaat caattttttg attttaggaa
      421 gagtgagatt tcagaattat taaaggagct aattgattta gaagatgatt tttatgatta
      481 taaacatttt agaccaatta tgaatataga tatatttcat gaatataaca aagtttacat
      541 agctaaaatt ccaacaatta aaccatatga agtatttgca tacataccta ttggcggatt
      601 taatgattgt cctagagatg aagaacatat agctattgca aaatattggt atgaaaagca
      661 tggaatcttt ccaatcgcaa ttggatgtga tacagttcaa tattctgtaa aaaatttaac
      721 taaagatggt aaaaaatttg atgacttatg tattgaatta gtattttatt gtgaggatat
      781 tattatacaa gggtatgaaa ccttaaaggc tcttaaggat gtattaaaac gctctacaat
      841 agtgcttttt tggtgggatt gatattctat catttagata gtattaaatt gttagtcacc
      901 aagaatctac ttgctttaat tatggggagt gtgtcaaact aggtttttat acacagtgta
      961 gcatagtaat atgtttaaat tacaaaggtt aaaaaaatac tttaatattt ttaatattat
     1021 aattaataga tttaaaggta attataattt ataaaataaa tactttatat gttataatca
     1081 ttaagaatgt aaaaaaataa tatatagttt tatagttcat atttttccag atatggaggt
     1141 aaattttgtg aaggaaaaag aaaaattaga taaattaaaa aaaatgttac ttgaattagg
     1201 cagtgttgta gttgcttatt ctggtggagt agatagtaat tttttgctta aggtcgcaaa
     1261 agatacatta ggtgaaaatg ttatagctgt gactattcat gcaatgatgc attcaagtag
     1321 ggaaattgaa gaggctaaac aatatactca aaattttggt gttaaacata tcatattaaa
     1381 tatagaaaac tttgatttaa aagaatttaa agaaaatggt gttgatagat gttatcattg
     1441 taaaaaatat atattctcta aaataaaaga ggtagcaaaa gaacataata taaagtacat
     1501 agtagatgga acaaatatag atgatttagg agactataga cctgggctta aggcattgag
     1561 tgaattagga gtaattagtc cattaaaaga tagtagttta aaaaaagaag aaattaggtc
     1621 gctttcaaaa acattaggat taaatacttt taataaacca tcatttgcat gtcttgcgag
     1681 taggatacct tatggagttg aaataacaga tgaaaattta agaatagtag aaaagagtga
     1741 agaatatcta tacaatttag gtttttctca atttagagtg agaatgcatg gagatatagc
     1801 tagaatagaa gttggacagg aagagctagg taaatttttt gaaaataaca attttaataa
     1861 agttgatact aaattaaaaa tatttggctt taaatatgtt acactagata tgtcaggata
     1921 taaaatggga agtatgaatt taaatgtgta gattatttta aaaataaata agttttatag
     1981 ttaatataag gaaacaggag gttttatgaa aaattatacg ttaatatcgc catgtttttt
     2041 tggaatggaa aaaatgctgg ctagagagat tactaattta ggatatgaaa taataaaaac
     2101 agaagatgga agaataacat ataaaactga tgagtttgga atagcaaagt caaacatgtg
     2161 gttgagatgt gctgaaagag tacatcttaa aattgctgaa tttgaggcaa aaagctttga
     2221 tgaattattt gaaaatacaa aaagaataaa ctggtctaga tatatacctt atggagctca
     2281 atttcctata tcaaaagctt cctctataaa atcaaaatta tacagtacac cagatgtaca
     2341 agccatagta aaaaaggcta tagtagaaag cttaaagaaa agttatttgg aggatggttt
     2401 gcttaaagaa gataaggaga aataccctat ttttgtattt atacacaaag ataaagtaac
     2461 catttctata gatacaacag gagatgcttt gcataagaga ggatatagag aaaaggcaaa
     2521 taaagctcca ataagagaga ctttagctgc tgggcttatt tatttaactc cctggaaagc
     2581 aggtagagtt ttagtagacc ctatgtgtgg ttctggtact atattgattg aagctgctat
     2641 gattggtata aatatggcac ctggcttaaa tagagagttt atatcagaaa agtggagaac
     2701 tcttgataag aaaatatggt gggatgtaag aaaagatgct tttaataaga tagataatga
     2761 atctaaattt aagatttatg gatatgatat agatgaagaa tctatagata tagctagaga
     2821 aaatgctgaa atagcaggtg tagatgaata tatagagttt aatgtaggag atgcaactca
     2881 atttaaaagt gaagatgagt ttggatttat aatcactaat cctccttacg gagaaagatt
     2941 agaagataaa gacagtgtta aacagttgta taaggagctt ggttatgcat ttagaaaatt
     3001 aaaaaattgg tcgtattatt tgataacttc ttatgaagac tttgaatatg agtttgggca
     3061 aaaagcagat aaaaaaagaa aattgtataa tggtatgtta aaaactaact ttttccaata
     3121 tccgggtcct aagcctccta gaaataataa ataagtttta catgatttat ttgtaaaata
     3181 taaaatttta ttaaaataag taaaacatta tgatgccctg aaacgtctaa ttattgtgag
     3241 gagatttttg tgaacaaatt taaagattta taggattagg aggagttaaa tgaaagtgtt
     3301 aaagcttatt actgtagtag ccctgatgat gttatttgtt actggatgta atataaaaac
     3361 tgaatctcca gaacaattag cgaagatacc tgactatgat gatacaaaaa aagtattgta
     3421 tgatggtatt gaccagttac taaaaccaga ttcatctatg atattgcctt ctaatacaaa
     3481 agaagtagga aaaataaata aggtagattt aaacagcgat ggaattgatg aattagtagt
     3541 atttgaacaa aaagaagatt taagtaataa tgtgagtcag gtagggtttg taaccttgag
     3601 ttacaatgga gaaaaatacg tacttggaga ccatttttta gaaaatggcg aatcaataga
     3661 atatgctaat ttttatgatt tggactcaga tggatataag gaagttatct tattggttaa
     3721 atccaaagac aaaacgaata tgcatatata taaagtagta gataatgaaa taacaaaaat
     3781 atatgacttg gatgcatcat ggcttcaaaa taaagaagat tttaatgaca tgaaagtaaa
     3841 aattggatat atagatggag atgacaagtt agacatattg atgcttcatt tgaataataa
     3901 gactaatgaa atgtttgcaa gtgttgcaaa ttttgatggg aaaatgaaaa tcaaagattc
     3961 tgttaagttt gaaaatgtaa aaaacttaag tgaattatat ataacattag gtaatgttgc
     4021 ttcatctgtt ggaaattctt ctgcagctat aaaaggtatt gtacttgata ttcctatatt
     4081 gaaagataat aactatatta cgcagatact ttatatgaaa gatgggaaaa ttaacaaagc
     4141 ttttagtgat tatgataaaa ctattactaa atcttattat atacctgtag atgatattga
     4201 caaagataag attatagaaa tacctatagt tgcaggcagt acaggtaata ataaaaatac
     4261 ttatagttct aaatcatctg caactataag ttggtataga tggaatggaa aagaaggtgc
     4321 tgactcaagt ttaatattta caagtcagat atattataat tataaatata attttaaatt
     4381 atttattcca aataatttat ttgataaaat acttgttgaa caggaatttg ttggagaaaa
     4441 agcagtattt aaattttact attttgacta ttcagaccgt aagaatttat ttaatatagt
     4501 agtagagagt aaaaataaac ttgaagatag aaaaaataca ggtactcaaa attctattgt
     4561 acttcaagag agcgatgaat atactttctt actagttgta aacaatgcaa atgaaatgga
     4621 taagctagat atgactatag acgcattaaa agaatatttc tcattaatat atggttaata
     4681 ttatactcaa ttggaggttt tattttatga aggaaaagat acttatacta gaagatgaaa
     4741 ttggtattag aagttttgtt agtataaact taaaaaggga aggctatgaa atagttgagg
     4801 caggtactgg aagagaagct atagaaaaaa tgacaacaga aaaggatatt acaatagctc
     4861 ttttagatgt aatgcttcca gatataagtg gtatagaagt atgtaaattt ataagggaga
     4921 attttgacca agtaggtata ataatgctta ctgctaaagc tcaagaagat gataaaatag
     4981 aaggatttat ctcaggagca gatgattaca tcataaaacc atttagtata aaagagcttt
     5041 tagtcagagt ttctgctctt cttagaagag tagcaaagga tgattcaagt gttaagtcga
     5101 gtgagatagt ttctccacca tttatacttg atatagacaa aagaaaatta tttaaaaatg
     5161 gaaaggaaat tgagcttaca ccaactgaat tttcaatagt gaaatatcta atatcaaatg
     5221 ctaagcagtc gttaagtaga gaccagatat tagacgaagt atggggaact aactatttat
     5281 atgattttaa aatagtagat gtaaatataa gaaggataag aaataaaata gaagatgacc
     5341 catcaaaacc aaaatatata caaactattt ggggttatgg atactgtttt agaaaggaag
     5401 aataatagtg ttacattttg aaggtctgag aaaaaaagta ataaaaaact attttataat
     5461 aattattata atggttactc tgtttgaagg attgttcatg ttttacatac aaaactacta
     5521 ttatgattct gtcaaacagc tacttgaatc tgaaataaaa tatgctgatg aatataatgc
     5581 tattacaatg gaaactacaa gttttgagaa aaaagtaaaa aatatatttg ataaacaacc
     5641 cttaactaaa aattcagaat ttggaatttc aataatagat aaagataaaa atattatatt
     5701 agaccaatat ggttttaaaa gtaaagaaaa agcaaactat gaagatgtta acaatgcatt
     5761 aaaagatata aaaactaaaa atttaacacc ttatacttat agaataccag atacagggga
     5821 gcatgttatg agtatctcct taccacttaa ggtaaataat ataatagaag gtgttgttag
     5881 atatacagta tcattagatg ctattgataa tgcgatatta aagcaagcta catggcttat
     5941 attagcagga atatttatac ttattatagc tatactaata agtcttaaat ttgctgagac
     6001 tcttataaaa ccattacgag agctaaagaa atttgcaaat gaattggctg taggaaatta
     6061 taatataaaa ttagaaaaga tgaagattgt agatgatgaa attggtgatt tagcacagac
     6121 atttgaacac atggcacatg agattgacaa aagtgaaaaa ttaaaagagg aatttatatc
     6181 atctgtatct catgaactaa ggactcctct tacatctata aaggggtgga gtgaaacctt
     6241 aggttatgaa agtataacaa gagaagaact agacttaggt ttaggcatta tacaagatga
     6301 aacagaaaga cttataaaac tagttgaaga attattagac ttttcaaggc tttcttcaga
     6361 caggataaaa cttcatgttg atatagttga tgttgaaggt ttaattgtag gtgttgtaaa
     6421 tcaacttaaa gttaaagcag ctgaaaaaga tatatcactt ttatttgaat ttgaaaatga
     6481 atttatagag aacattcaag gagataaaaa tagattaaga caggttctca taaatttaat
     6541 acaaaattca tttaaattta cgagtcaagg tggttacata aaagttgtag cttctcaaga
     6601 tgaagagata acaaccatat cagtagagga taatggttca ggtatagaaa agcaaaattt
     6661 aaataaagtg ttagataaat ttttccaaga agattataat aaggctggta gtggattagg
     6721 actagcaatt agtaatgaaa ttgtaaaact tcatggtgga agaatgaaaa tagaaagtga
     6781 aaagaatgtt ggaacaaaaa taacttttaa tataaaaaat aaatttgcta aacaagcata
     6841 aattcttaga aagaggatta agatgagaaa cataaaaatg atagtagcat acgatggttc
     6901 aagatataaa ggatatcaaa aattaggtga caataatatg actattcaag aaaaacttga
     6961 aaatgtttta agtaaaatga ctaatgaaac agtggagata ataggttctg gaagaactga
     7021 tatgggagct catgcaagag gacaagtggt aaattttaga actaattgta tggattcttt
     7081 ggataaaatt caaaaatatt tatatgaata tttaccagaa gatatagttg taaaaactgt
     7141 tgaagaagtt gatgaaagat ttcattcaag atacaatgta aaatcaaaga cttatatgta
     7201 taaaattgac aataataagt atcataatcc ttttataaga aaatatgcta ctcatgtaag
     7261 taaaaaatta gatttagata gaatgagaaa agcaagtgag tatttagtag gagaacatga
     7321 ttttactagt tttgcttctt ctaaatctaa gaaaaaatca aatgtaagag aaatttattc
     7381 tataaatata aaagaagatg ataatgttat agaaatatat gtagaaggaa atggattttt
     7441 gtataacatg gtaaggataa tagtaggtgc attgatagat gttggtctaa aaagaagagc
     7501 tccacaagat attaagtata tgcttgaatc aaaagataga tgtcaatcat cagatactgc
     7561 accagctaaa ggactatgtc tttggaaagt aacatattaa tcaatcatat aaaactctct
     7621 ttaaaataaa aataaaataa attatatagt tgacataaat ggaaaaataa actattatat
     7681 attttataag agagatataa aactttatat ttaaatcctg tgagaaagag agtaacttct
     7741 atgtgtttta tagagagctg aggatggtgg aagcttggca aataagtatt agtgaaaaga
     7801 actttggagg atactaattg aatttagtat gttagtaggt aatctcgcct taagagaaag
     7861 agtggataag tttatcttat caattagagt ggtaacgcgg atatatttcg tctcttggtt
     7921 tttccaagag gcgtttttta atatatttaa ttattagtgt ttatctggta tgttgaacat
     7981 ttactaattg attagctaag tttatcaata agagtggtac cgcggatatt tcgcctctta
     8041 gtattttaaa atactaaggg gtttttttag tattaaaatg attttatgtg agtctaaatt
     8101 ctcagagtca ttcaatatta agaatttcta taaaataaaa attataattt aagattggga
     8161 ggaattaaaa atgaaggaaa aagtagtatt agcatattca ggaggacttg acacatcaat
     8221 cataatacct tggttaaagg aaaattatga agatatagat gttatagctg tttgtggaaa
     8281 tgtaggtcag gaagataaaa tggaagatgt atatgaaaaa gctttacaaa gtggagcatc
     8341 aaaggcatat gtggatgata taagtgaaga atttgtaact gaaactatat ttaaagccgt
     8401 aaaagcagag gctaaatatg aaggaaaata cttattagga acttctttag caagacctat
     8461 aatagctaaa aaattagttg aagttgctca taaagaaggt gcaaaatata tatgccatgg
     8521 atgtacagga aaaggtaatg accaagttag atttgaagct acaattgcag ctcttgaccc
     8581 gactataaaa gttattgcac cttggagaat ttgggatata aaatcaagag aagatgctat
     8641 agactatgct gaaaaacata atataaaggt aacagcaact aaagctaaaa tatattcagt
     8701 agatgctaac ctttggcatg tttcaacaga aggtggagat atagagcacc tagaaaatga
     8761 acataaaaaa gatgtttata aacaatgtgt agacccagaa gatgcatgtg atgtagctga
     8821 atatgtagaa gtttactttg aaaaaggtgt acctaaaaag gtaaatggcg aagaattatc
     8881 tccagttgca ctgattcata agttaaatga gttgggttgt aaacatggaa ttggtgttat
     8941 agatatagtt gagaatagac ttgttggtat gaaatcaaga ggtatatatg agactccagg
     9001 aggaacaatc ctttatgaag cacataatat tttagaaagt gctactttag acaaagatac
     9061 attacatttc aaacaaatgg tatcttataa atacggagaa cttatttaca atggactatg
     9121 gtattgtaaa ttaagagaat ctatagatgc atttatggaa caaactcaag ataatgttac
     9181 aggaacagta aaagttaaac tttacaaagg aaatataaaa cctgctggaa tatttactga
     9241 aaatgctctt tatgatgagg gaatatcttc ttttggaaat agtgaacttt atgaccacaa
     9301 agatgcagaa ggatttatta atttattcac tttaccatta aaaatcagag ctatgaaagc
     9361 tggaaaataa taaaataaat tatttaagag tgtataaaaa ctatttttag gtttttatat
     9421 gctctttttt tatttgaatt tattaaatta tacagtaaat agtataaaaa aatatattat
     9481 agttccaaat ggcttaataa tgtgaaaata taagacctaa aatataaatt ttataaatac
     9541 aatagttatt gactctctca ttgtaagagg gtttatactg aatctaatga aaatttatag
     9601 tgaggagata gattatatga gaaaaataat tgaaattaaa catcattcac atgattatga
     9661 gtgtatgtgg aatggtatag aagatttata tataaataaa accggtgaat gccttccaaa
     9721 cgattttttc tttttactgt caggttttgg ttcattttgt tatatgaaaa caaataaagc
     9781 tgatttgaaa agaatggtag ctttgggaga tggaaggaca aaacagatgt ataaattttt
     9841 agcaccaatt gttggatttg aatacaaaca tcatgagttt aaaaagtatg agcaagcatt
     9901 aaagaaagca aaatctgaaa ttgatgatgg atatccagtt attttaggag ctttagatat
     9961 gtattatcta ccatattata agaagctata tcataaagaa catattcctt ttcattatat
    10021 attaatggta ggatatgatg atgaaggaat atatctgtat gattgtggta gaacagaatt
    10081 attacatctt tcttatgata aattacgtga aagcatgaat tgcaattccc ctggattaag
    10141 caaagaaaat actatttgca caataagaat gaatacttca aagaataaat ataacattgc
    10201 tagagaagct attgctataa agcgtgatat gtttttaaat ccacctaaag gatttttggg
    10261 atataaaggt tttgaaaagt ttattaaaga gttgccacag tggaacactg aattggataa
    10321 agatgattat gataagattt tgaccaatat ggttatgttt tttggaacag taccaacaat
    10381 tccaaatgca atcaaaggaa ttaaagaacc agatactgtt caatttaaag gtggatttga
    10441 taaaatgagt agaatgctca acagtattgg ggaggaatac agaaacaact cttttataca
    10501 agtagctgat atttttgata aaactgctat tataattgag aaaatatcca atattataat
    10561 agattactta actcaaacat gtgatgatac agaacaactt ccgttattgt tctctgaggt
    10621 cttagagcat atgaaaacag gatatttaat cttggatgta taaaaaatat attgttgacc
    10681 ctaacattac gtaatagtgt aatatgaaaa tatagcttaa atatattagt aggtattgat
    10741 ggctgtaaaa ttaaaatttg aggtgtggat atgagaacag tgaaacaagt ttctgattta
    10801 actggaataa gtgtgagggc attacattat tatgatgaaa taggtctttt aaaaccaaat
    10861 aaaattacag atgcaggata tagactttat gatgatgaat ctattaaaac tcttcaacaa
    10921 attttatttt ttaaggagat tgatattcct ttaagagagg ttaaggaaat aatgtcaagc
    10981 caatattttg ataaggttga agctctaaaa aatcaaaaaa aattacttat tttaaaacga
    11041 aagagactgg atgaattgat agagcttata aatcaaactc taagaggaga aggaaatatt
    11101 gattttaaag agtttgatat gagcgagtat tttgatgttt tagaagaatt taaaagagag
    11161 cataggaata aggtaattaa aatttatggt agtgtagaaa aatacaatga atatatagaa
    11221 agagttaagt ctaatgaaga gaaaattgct aaaatggcta taaaacaata tggaactatt
    11281 gagaagtttg ctaaagctat aaaaacaaat tttagcagtg atatattgaa tttaggagaa
    11341 aaatttgata gatataaaaa tgattgttta aaagaaaaac atccaaaatt aaaagaatta
    11401 tatagaaagc ttgtagaaga tttaagtagg aatccttctt caacagacct tcaagaaata
    11461 gcaaaagaga taacagatat atctaaaaaa gattatgaaa ttttctctat ggatactggt
    11521 gatgacaact ggtattatat ggttcaaaat tatctagtaa atcctatgtg gatagaagaa
    11581 gtagataaaa agtatggaag tggagcagga aagtttattg gacaagtact aaagacttat
    11641 ctaagagata gaaaaccaaa gataaataca ttgtatgaaa aacttgtaga agatttgagt
    11701 agagattgtt cttcaaggga agttcaaagc attgttgaag aaatagacaa tgaaatgaaa
    11761 agaagtaatg aattttataa aatagacaat ggtgaaagat actttgatta tatgtcagag
    11821 ctttatttac aagattctaa ctatataaaa gtaactgata agaattatgg tgatggtgca
    11881 tctaggttta ttggagaagc ttttaagatt tattttgata ataataattg ttgaaaattt
    11941 tgaagtaaaa aatatgtaag ttttataaaa gcacaaaaat aaggatgtag attagctcca
    12001 tctatatcct tatttaaagt tattaaaata tatagtttac atcaaactta acactttcaa
    12061 taatatttcc caaatatgtt tttatacttt caacttcttc taaagatttt aatttatttt
    12121 cagtataaat tgctttgtcc agaatattaa accttaatcc tatttcatca aagtactcat
    12181 gatttaaaac tttgtaccaa tggttttttt ctttattcca actcgtagat attgaatcag
    12241 cttctttttg agctttttcc caatcttcat tttctataga tttttctaaa acagatatat
    12301 tgtctttata tccttctgta aaatcatata gtttattgtt aacgtaaaat ccaaataata
    12361 caaataagat agtccatagt ataacaaatg ttgtagattt caatattaat cacccttttt
    12421 attttgaata aatattttgt cattttcatc taaaacacaa ataagaacat ctttaaaagt
    12481 ttctatgtga ttagatttta aaatacccat taaccagtta gtatcttttt gagctatgtc
    12541 tagattttta tttattattc taccatccag tataagtggg ataggtaaat gattaaaagc
    12601 tcttggagga gtactattgt agcttgatgc tggtacaaca cttagattac catcagtctc
    12661 caaaatagca tattgtacat cttttaggtt aaagtatcct tgaatcctaa gttgttctaa
    12721 taattcatct atactaattc tttctttttt aagctcttta tatacaattt tccctttatc
    12781 tattaataca gaaggtttac ctgataaaaa tgaacttaat tttctacttt ttaatgataa
    12841 gaatgaaatt agagtctgca tgattacaag tccagatatt gctgcaacac catttatgat
    12901 tggtatatta ttattttcca ttgggacaga tgcaacttct gcaattaata gtgttataac
    12961 taaatcaaaa cagttcattt cagctatttc accttttccc atgactctaa gtgcaattag
    13021 cactgttatg tataatataa tacttcttat taatactact gtcatatcta ttaattactc
    13081 ctttaaaaaa tatttacact tagttagtat atcctatata aaaaatttaa ttcaaagtat
    13141 aaaaaacctt aaataattgg attcaactat ttaaggtttt tttgataatt ttaatattaa
    13201 attttttatg gattttctgt tacttttatt tattttaata acagattcaa caaaactctt
    13261 tttatctcct aattttaaga taaaatcagg atattcttca aattcattga gttttattaa
    13321 tttgaaagaa gaagatagat ttttaaagtt aataatttta gagttattca ctaatacact
    13381 tcttttttta aaataaattt cttttttact tatatttagt atagttaata tttgtttaag
    13441 atatattatt atatcttctg gagttctatc aaatttgagt tcaaaatttt catcttcaaa
    13501 gtagggaata taatataaag gtgttttgag agcaggtaat ttattatata attcttcaag
    13561 aatatcgata tttttagggg agttaaagaa aaagcattta catacattca ttaaatccac
    13621 agcatctgaa agtgagttat gtttaatatc tccttctaca gaaaaaattt ttttcatatt
    13681 cagtaaagat atttgcttag ataagattct attattgtat gtaatatgtt gagatataaa
    13741 aggttgaatg tcaatcatca tattaaataa ctctaagcat ttacctgtat atttatatac
    13801 tctaaatgaa gttagaaggc cagttatatc aaaatcaccc caattatata ttgtaacatc
    13861 atcaaattta caaaaccatt tataaaaatc atccataaca tataaaagat taggagcatt
    13921 attgatatct agttggttca atttggttaa atttttacat ttatttgaaa gtattttatt
    13981 gtattttggc ttaactaaag agtaataatt atctattgta ttaaattcgt tatcaactaa
    14041 aactccacca atagaaatta attccgcacc attatattct tttggtttat ttttattatc
    14101 atctagtata ttaaattcaa agtctataaa taacttgtat ttcataaatc taaagcacat
    14161 cctcaggtaa attatttatt gagcctaagc atcataagaa tcaatagata taatgactct
    14221 aaacatcaaa ggttcattac aacttttatt tatttcataa tttaattcaa ataaatcttt
    14281 taagatataa tttataagta ttctttccca acccattttt aaattaactt tagagtctat
    14341 taatatattt tctatctttt taaatatttc tatatttgct ggtattatac tgagctcaat
    14401 attactttta aacgggttag actgtaaatc tgttataagt atatttttta attcctctgt
    14461 aagttcagaa attaaactat cacctatgta tttttttatg atttcctctc taatttcttt
    14521 taaatttagc aatattagta cctgctactt acatagtagc ttttcctttc cttttaatac
    14581 tattattcat ttttgaatct tatttagata tattttaaga ttaaaagtct ttagctatat
    14641 aattttttac ccatttttaa cttatattaa catattacag gcttatatct ccaccctgtt
    14701 tacattgagg atggagtata ttgcctattt tgataaaaat gataggttgc tatttgccta
    14761 ttcctttttc tgataatttt tctaacatat ctttggtcat tttatctaaa tcataactaa
    14821 acttaaagtt ccattctttt actgcactac tactgtctag agagttaggc caagaatcag
    14881 ctatactttg tcttacaggg tctacatcat attctatcac aaaatcagga atatattttt
    14941 ttatagaatc agctatttct tcaggagaaa agctcatagc agtaatatta aaagcatttc
    15001 tatggatgag tttatcagct ggagcttcga ttaaatccac tattgattga agtgcatcag
    15061 gcatatacat catatccatt tttgtacctt ctgctatgta agatttatat ctcttatttt
    15121 ttaatgcctc ataatatata tcaactgcat aatctgtagt tcctccacca ggaggcgtaa
    15181 cataagaaat aagacctgga aatctaacac ctctagtgtc tactccaaat tttgagtgat
    15241 aataatcaca tagtaattct ccagaaactt tagtcacacc atacatagta ttaggtcttt
    15301 gtaatgtatc ttgaggagtc atatgttttg gagaattttc accaaaagca gcaattgaac
    15361 taggtgtaaa aaatttacaa tccaattctt tggctatttc aagaccattt attaatccat
    15421 tcatatttaa gttccaagct tctagaggtt tactttcagc aactgcagaa agtaaagatg
    15481 ctagatgaat tatagtatct acatcatatt tttttgcaat ttcaaaaaaa cgattcttat
    15541 ctgtaacatc taatatctca aatataccag attcacatac tggattacct tcttttactc
    15601 tccttgaact agcgattaca ttttgttctc catactcatt tctaagcttt attgttaatt
    15661 ctgaacctat ttgtcctaat gcacctgtta taagtatttt tttcaatttt acaacctcct
    15721 aagggtttaa tttatgtagt actacttatt tttattttag caaatctttt gtttaaatac
    15781 aaagaattaa agataataat atacgactta attgaatgaa gtgcagtaag tgcctaaaaa
    15841 tttaaagcta atatataatg gtatattgta taacaataaa agagtagatt aaattattat
    15901 atatagattg tgtgactaag tcacagaaaa tagagttaat ttaatatata ataaaagcat
    15961 atcaagtaaa gctttaaatt aaagtaactt tatataaaaa gtaatataaa ttgataaaat
    16021 tataaattta tttatagtga atatgggttg gattttatag atacaaaaat agtatatatg
    16081 atatgtagac cattagcatg tttaaagtag caaaacatat aaaataaaat ttgaaaggaa
    16141 gtataaaaat gacaagacca tcaatatatc atagccaagg atacacttgt gcagaggcat
    16201 taataaagtc ttataatgag gaacataaca cagatatacc aatctcaata ggaagtggta
    16261 tgggggttgg aatgaatgtt ggaagtgttt gtggagctgt aaatgctgct gttgttataa
    16321 tagggtattt aaatgggcga aatagtaatt tagatgaaaa tatagcaagg aagtattcaa
    16381 gagagctatt gaatagagta agaaataaat acagctcaga aatatgtgta tctcttaaaa
    16441 aaaataaagt aacttgtgaa gaaataataa atttcacata tgatgctcta aatgatatgc
    16501 taaaaaatca aaaataattc taagaaacaa gtattttaca ttcgatagaa tacttgtttt
    16561 tttatgatat ttttaatagg tattagttta ttataatttt taaatcaatt ttattatatt
    16621 ttaattgaaa caatctactt tgaaaattac taactgacaa aagaatatga ttatagtata
    16681 gaaaaaatgt aaattatt
//
LOCUS       DBGJFF010000082        16541 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.083, whole genome shotgun sequence.
ACCESSION   DBGJFF010000082 DBGJFF010000000
VERSION     DBGJFF010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16541
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.083"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            126..794
                     /locus_tag="ACOIAF_002595"
     CDS             126..794
                     /locus_tag="ACOIAF_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HGZ2669599.1"
                     /translation="MSKLESTTEKVEVKWYGYVALILGALFFSGIFKDAPGPLKVLDF
                     NNVLGSFGTLGTINDGVGTLAANFRGDGGTGPRDGWLYALTLIPSVMFALGVVRVIDH
                     LDGMKAAQKLLSPLLKPLLGLPGFAGLTLIASLQSTDAAASMTKELKDDGYIDEKQKA
                     VFCAFQFSGASAITNFFASGAALFPFIGDVPIFIPLALILIMKFVGANLLRLYLNKFD
                     KGEA"
     gene            796..1281
                     /locus_tag="ACOIAF_002596"
     CDS             796..1281
                     /locus_tag="ACOIAF_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HGZ2669600.1"
                     /translation="MSEVAKKEVKVKKGNVIDAFIGGARNGFQISTNSMAPNVIFGFA
                     IISVFNLTGLLDVIGTIFTPVMSIFGLPGVAATAIMTIFISMGGASGVIAGLFTAGQI
                     SQAHVAILLPMLLLSGAQIQFMGRLLGTAELKTKYYPHMFVIATLNGCIAMLIMNYFV
                     V"
     gene            1342..2463
                     /locus_tag="ACOIAF_002597"
     CDS             1342..2463
                     /locus_tag="ACOIAF_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 peptidase aminoacylase family protein"
                     /protein_id="HGZ2669601.1"
                     /translation="MGTNKDYIEYVHKVYDYLHTIPEKGFEEYKTSEFVLKELEKMGF
                     ENINKGVAGTGIIVTIDSKKEGPVFAIRADMDALEFIIDGEKKMIHACGHDGHTSMLL
                     TAAKLIKERNLVKRGKLKILFQPGEEKLYGALRVIDSGLLDDVEEMVGMHLRPGQDCR
                     LGKAIPAMYHCASCIMEFTIKGITCHGSKPHLGVNAVDAVCLAVNAINAIRVNPAIPN
                     SIKVTNISVKGETYNNIPDEAFMALDIRAESNDIIEELKEKAIHAVKTAVSSINAEAV
                     LISKDGVPAAEFDEELVNLAEETIKDVLGADASIGIFKNSGGEDFHYYTQKLKCKTTY
                     IGLGADATPGFHNPNVTFDTKALEYGVDIWCRLVEKRLG"
     gene            2744..3934
                     /locus_tag="ACOIAF_002598"
     CDS             2744..3934
                     /locus_tag="ACOIAF_002598"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HGZ2669602.1"
                     /translation="MSKYNFDRVIDRVGTDCVKWDFRTNCSTKAQKDGLPFWIADMDF
                     ECAEPIIEALRKKVEHKIFGYSSNDSDKYFNAVCDWYKRRFNWEINRKDIIFSPGVVP
                     AVAILVRILTNSNEGVIVQKPVYYPFEAKIKSNNRDVVNNPLIYENGTYRMDYDDLEE
                     KAKCSNNKVLILCSPHNPVGRVWREDELKKVVEICKKYDLWIIADEIHSDLIRKGFKH
                     TPLQSLCPEYKDKIVTCIAPSKTFNLAGMQLSNIIVNNDELKKKYQEEVTAVGVATSP
                     NPFAIVATIAAYNESEDWLNELNDYLDNNIQFIDEYLKENLPKVKLVYPEGTYLAWLD
                     FTAYGLNEVELEDLMFKKANVLFDEGYIFGKEGIGFERINVACPQSLLKECMDRLKTI
                     FENL"
     misc_binding    4068..4323
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            4382..5383
                     /gene="asnA"
                     /locus_tag="ACOIAF_002599"
     CDS             4382..5383
                     /gene="asnA"
                     /locus_tag="ACOIAF_002599"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HGZ2669603.1"
                     /translation="MSTIIPKDYKSSLNVIDTQIAIKKLKDFFEMRLSNELNLIRVSS
                     PLFVLPETGANDNLNGEKAVSFDIPFMNKNAEIVQSLAKWKRLALKKYGFEVGSGLYT
                     DMNAIRKNEELDNIHSLYVDQWDWELVIDKSSRNEKTLKDVVKRLYGVFKDTEIFVCS
                     MYEGIKEILPEEITFITSQELEDMYPELSPKEREDKIVKEKKAVFITQIGKTLISGEK
                     HDGRSPDYDDWELNGDILFYNPVLDSALELSSMGIRVDEESLDRQLKLAGCEERKEFD
                     YHKMLLNGELPYTIGGGIGQSRICMYFLQKAHIGEVQVGVWPQDMIQNCSSAGIELL"
     gene            5487..5876
                     /locus_tag="ACOIAF_002600"
     CDS             5487..5876
                     /locus_tag="ACOIAF_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HGZ2669604.1"
                     /translation="MEFLKIIINIVLDILKKILVRFKNAKFGLVFVFDLLKLPDFMTD
                     KRINIVDKIKVISVLIFTISYFVSGVDIIPEMIAGAFGFIDDAIVLIWSIGIVNEEIN
                     KYRVIAKKDKHSNIIENVEFSIKDEEE"
     gene            6037..7245
                     /locus_tag="ACOIAF_002601"
     CDS             6037..7245
                     /locus_tag="ACOIAF_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HGZ2669605.1"
                     /translation="MKKKIGLVPKLIIGIIVGILIGSYAPEIIVQILVTVSTLFSAFL
                     KFVIPFIIIGFVTAGIADLATGAGKLLGITTGIAYGSTLIAGLLSFAVSTLIFPNFID
                     ASVASQIGDPEAGMLAPIFTIPLEPMVDVTAAIVFAFVMGLGISALRNHGKGETLFNF
                     FQEFQGIVTKTLSTVIIPLLPLYIAGTFANITIAGEVWTILGVFWKVYLVVIPLHFVY
                     MACQFTAAGVFSGKNPVRMLKNQVPGYLTAIGTQSSAATIPVNVGCAEKNGVSKQIRE
                     FVVPLCATIHLSGSMISITGFTTAVLMMNGMPHGIGVLFPYIAMLGIAMVAAPGAPGG
                     AIMSALPFLPMVGIPSDGGLASLMIALYLTQDSFGTACNVSGDNAIAVVVDKINDGMS
                     KKGNKEEQSA"
     gene            complement(7331..8407)
                     /locus_tag="ACOIAF_002602"
     CDS             complement(7331..8407)
                     /locus_tag="ACOIAF_002602"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003415975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HGZ2669606.1"
                     /translation="MSTNHGANLYSLSSKYGFSKEEFMDFSSNINPFGTSSLAKQYIV
                     NNIDMVSMYPDPDYIDLKTSISNYCKCSIDNIVLGSGATELISSFIHTINPKQALLLS
                     PAYSEYEKELSKINCSIEKYFAKEEDNFHINLENLIKTINAKDYDLVVICNPSNPTGF
                     AFTKVEVREILKNTDSFLMIDETYVEFTDTDTYSCTQLVDDYSNLFVIRGTSKFFSTP
                     GIRLGYGLISNTNVKNEINKNLDLWNINIIASKMGEIMFSDLDFISNTISLMNTERNY
                     LLKELKNIKSLDIYNTKGNFILCKIKTKELTAKSLREQLLPQKIIIRDCCSFEGLDEY
                     FFRVCILKPNENKLLISFLKAIFK"
     gene            8636..9163
                     /gene="hpt"
                     /locus_tag="ACOIAF_002603"
     CDS             8636..9163
                     /gene="hpt"
                     /locus_tag="ACOIAF_002603"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HGZ2669607.1"
                     /translation="MYKVTGKMLTEEQIKEKVYELGKKIEEDFKGEDLLVVGILKGAS
                     VFVSDLIRCIDLDVNIDFMSVTSYGNSTESSGTVKILKDLDVDIEGKNVLIVEDIIDS
                     GLTLSNLVAALKTRNPKSLKLCTLLDKPQRRKANIPVDYVGFVIEDKFIVGYGIDYAE
                     KYRNLPYIGIVEDVE"
     gene            9252..10184
                     /locus_tag="ACOIAF_002604"
     CDS             9252..10184
                     /locus_tag="ACOIAF_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HGZ2669608.1"
                     /translation="MDIQLNILLFFQNLRNPILNFIFLVFTISTEAPLLILITTIIYW
                     CINKKCGQKILFAIIGNFVVNLGIKEFVKAPRPIGIKGLESLRVSTAGGYSFPSAHTQ
                     TATTFWVSIMTIFKKRYLHLIGAVMVLGVGLSRLYLAVHWPIDVIAGWILGIFFTVVF
                     VKIFDYIDDNKNYILLVVLLIPFIIVAIFLNSPEYFEKFGIIVGFVFGYMVEDRFVKF
                     NTDNNNKKINFSNKNKGSKNIIFSRICRFLVGIVTIGMLYIGIKLFISMLIVTLNISD
                     STMSIIFMNFIKNTVVVFYGIAGAPALFKLLKLN"
     gene            10246..11472
                     /locus_tag="ACOIAF_002605"
     CDS             10246..11472
                     /locus_tag="ACOIAF_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HGZ2669609.1"
                     /translation="MKKVVIIGGGPAGMIAASTACEKGYDVTLIEKNHKLGKKLAITG
                     KGRCNITNACEIEELIENVPTNGKFLYSAFYTFTNDDVISMFNNLGVKTKTERGKRVF
                     PESDKAFDIVNALERQLKSKKVNILLNSKVEKIISKNNKIEKVILNDKKEIKCDSVVV
                     ATGGLSYPLTGSTGDGYKFAISQGHTIIDTKPSLIGIEVQESFTKDLEKLSLRNVEIR
                     VFNSKQKKVYSDFGELEFTKFGLDGPIIKSASCRMKDTRKENYTILLDLKPALDEEKL
                     DKRVQKDFQKYTNKKFEKALDDLLPKKLIPIIINLSEINPNTVVHQISREQRKNLVHL
                     LKNLKFTVKRYRPIEEAIITSGGVKVNEINSSTMESKLVEGLFFAGEVIDIDAYTGGF
                     NLQIAFSTGYLAGFNC"
     gene            11556..11777
                     /locus_tag="ACOIAF_002606"
     CDS             11556..11777
                     /locus_tag="ACOIAF_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HGZ2669610.1"
                     /translation="MASNNNNNRTVVPGAKEALNQMKLEIANELGMSNYQQVDKGNLT
                     ARENGYVGGYMTKKLVEMAEQQMAGKSNR"
     gene            11966..12136
                     /locus_tag="ACOIAF_002607"
     CDS             11966..12136
                     /locus_tag="ACOIAF_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669611.1"
                     /translation="MSSVNFEDLKNKFINSDLDEKIRIYTTTEGLSVEQFKELLKYYP
                     IQHLSKLEKALG"
     gene            complement(12229..12690)
                     /locus_tag="ACOIAF_002608"
     CDS             complement(12229..12690)
                     /locus_tag="ACOIAF_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669612.1"
                     /translation="MQIEQLDLETRNKIYCYTKKILRKYQKGITSGKLTADKFADNIL
                     SQDFISSILNEKIVNETNFKISYRNYIETLINIQNENLSNLRKKSTKTAKCFNISQIT
                     QLKNLLSNTGYNLLIPYKYLTARDIEGIVTLINTGSIELGNERIYNYISKA"
     gene            12879..13340
                     /locus_tag="ACOIAF_002609"
     CDS             12879..13340
                     /locus_tag="ACOIAF_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669613.1"
                     /translation="MLNGIIKLFYSEIVKTKINNLDSSGYSLRDECKEIRNITLKIDS
                     VFFKLLKLAFGSILLMVAFNLNMVLGIGVFLVGVVYILYKRNMEEQVKEHINNVKNNI
                     DISKITFMDEKGRTGLNALITLLLIGLLTGFNWILVASFVVVFVFTIKNVC"
     gene            13383..15146
                     /locus_tag="ACOIAF_002610"
     CDS             13383..15146
                     /locus_tag="ACOIAF_002610"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing SpoIIE family protein
                     phosphatase"
                     /protein_id="HGZ2669614.1"
                     /translation="MTNGSYKMKNLVEISAMVTESTDFFNIKDDIIEKMLEVVHPTKA
                     CVNLFYKNDSKYAYLVCSQTLEYVPQIFPINSLKGAKIDFDTYPEYIHEAVNEKKIIY
                     VENVFEDSRAEGERDLAKAEGYIGRIVFPFIINNVVVGFMTCFLKEDDYITEQDIDFI
                     SSVASLLSLSIEITNKNNNVQILIDKLRGSISAINEATKKLYLNKSIDGFLENLSKQA
                     CNITNSKEALIIINSHHNKDAIVSCYNVEQKKKTNLYPMIDMFLKKESLGGYLNNLKP
                     CSKKGINLESYIYYKLQDKNETIGCIVCANSKNYTSDDLNILSILSKQVSVGMQLYEH
                     NEKEVKHKVLENELNILNKQQQLIMDKGKMDCNDEKELYFYHKPARVVGGDFYYATKV
                     DDEHIVYIVADVMGHGMVSNYVVAMIKGAFKVLCNQYNTPREIMTNLNRMLYDEFDKM
                     GVFTTCLIGIIDTKENMITVSNAGHYSPVVVKKDGTIENNLNCKKGIPIGIMEDATYE
                     NNTLSMEDYAMVCMYTDGILEIKNSSKEEYGINRLENFLKENFRLNQQLIVENLKLDL
                     KNFSSKDNYDDDILIVMLKDR"
     gene            15154..15762
                     /locus_tag="ACOIAF_002611"
     CDS             15154..15762
                     /locus_tag="ACOIAF_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HGZ2669615.1"
                     /translation="MDSNDKKGLKSPEIIIINGSPRINKNCSFIANEVKAMLEECGLS
                     CKIFNIYNMDIEYCTACGFCEKTGYCKFNDDMKPLYKMFDDSKGTIVISPVHFDSISA
                     KLKTVVDRTQAIYASKYVLNKPSIDRSKKRIGMYIAVGGSKSYDSQFKGGQIVMDFFF
                     KSINTKLLHNIYLNNTDDVSYIDNQEFKDIIYKNLKIYIDSI"
     gene            15834..16403
                     /gene="pduL"
                     /locus_tag="ACOIAF_002612"
     CDS             15834..16403
                     /gene="pduL"
                     /locus_tag="ACOIAF_002612"
                     /EC_number="2.3.1.222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HGZ2669616.1"
                     /translation="MKLPIALSNKHVHLSQADIDVLFGAGHELTKFKPLSQPGQYACE
                     EKVDLVGPKGTLKGVRVLGPARAHTQVEISLADGFPLGVKAPIKESGDIAGTPGVKLV
                     GPAGEVEMKEGVIVASRHIHMSNEDAAKFGVTDKQIVKVKTSGPRALVFENVLVRASD
                     SFNLEMHVDMEEGNAAGVKNGDLVELIVD"
BASE COUNT         6468 a         1677 c         2924 g         5472 t
ORIGIN      
        1 atcgctggaa taaatttaca ataaaaaaat aaatacaaag gctttatcat taaggtattg
       61 gcataagttt tgcttattaa acaagtatac taataaaatt catttaaaca ggagggagaa
      121 tttatatgag taaattagaa tcaacaacag aaaaagttga ggtaaaatgg tatgggtatg
      181 tagcattaat attgggagca ttattttttt caggaatatt taaagatgca ccaggaccat
      241 taaaagtatt ggattttaac aatgtattag gttcttttgg aacattggga acaattaatg
      301 atggtgtagg tacattagct gcaaacttta gaggtgatgg aggtactggc cctcgagatg
      361 gatggctata tgcattgaca ttaataccat cagttatgtt tgcacttgga gtagttagag
      421 ttatagacca cttagatgga atgaaggcag ctcaaaagct tcttagtcca ctattaaagc
      481 cattattagg gttacctgga tttgcaggac taactttgat agctagttta cagtctacag
      541 atgcagctgc atctatgact aaagaactta aagatgatgg atatatagat gaaaaacaaa
      601 aagctgtatt ttgtgcattt caattttcag gtgcgtctgc aattacgaat ttctttgctt
      661 ctggagcagc actatttcca tttataggag atgtaccaat atttatacca cttgctttaa
      721 tattaattat gaagtttgtt ggggcgaact tacttagatt atatttaaat aaatttgaca
      781 agggggaagc ttaacatgag tgaggtagca aaaaaagaag taaaagttaa aaaaggaaat
      841 gtgatagatg cgtttattgg aggagctaga aatggattcc aaataagtac aaattcaatg
      901 gctccaaatg tcatcttcgg ttttgctata atttctgtgt tcaatttaac aggtctttta
      961 gatgtaatag ggacaatatt tacacctgta atgagtatct ttggacttcc aggtgttgca
     1021 gcaacagcta taatgacaat atttatatca atgggaggag catctggagt aatcgcaggt
     1081 ttatttacag caggtcaaat aagtcaagca catgttgcaa ttctattgcc aatgttattg
     1141 ctttcaggag cgcagataca atttatgggg agacttttag gaacagctga gttaaaaact
     1201 aaatattatc cacatatgtt tgttatagca actttaaatg gttgtattgc aatgcttata
     1261 atgaattatt tcgtagtata ggtaattata ataaaaattt aatataaatg gaaagtttaa
     1321 attttaagga ggagctagat tatgggaact aacaaagatt atattgagta tgtacataaa
     1381 gtgtatgatt atctacacac tataccagaa aaaggatttg aagaatataa gacatcagag
     1441 tttgttttaa aagagcttga aaaaatgggg tttgaaaata ttaataaagg agttgcagga
     1501 actggtatta tagtaactat agattctaaa aaagaaggac cagtatttgc tataagagca
     1561 gatatggatg cattggaatt tatcatagat ggagaaaaga aaatgataca tgcttgtggt
     1621 catgatggtc atacttctat gcttttaaca gctgcaaagt taataaaaga gagaaattta
     1681 gtaaaaagag gtaagttaaa aatacttttt caaccaggtg aagaaaaatt atatggagca
     1741 ttaagagtaa ttgattctgg tttgttggat gatgtagaag aaatggtagg gatgcattta
     1801 agacctggac aagattgtag gttgggaaag gcgatacctg caatgtacca ttgtgctagc
     1861 tgtataatgg aatttacgat taaaggtatc acttgtcatg gttctaaacc acatttagga
     1921 gtaaatgcag tagatgctgt ctgtttagca gtgaatgcaa taaatgcaat aagagtaaat
     1981 ccagctatac caaattctat aaaagtaaca aatatatcag ttaagggaga aacttataat
     2041 aatataccag atgaagcatt tatggcttta gatataaggg cagaaagtaa tgatataata
     2101 gaagaattaa aagaaaaagc aatacatgct gtaaaaactg ctgtaagttc tataaatgca
     2161 gaagctgtac taatttccaa agatggagta ccagcagcag aatttgatga agaacttgta
     2221 aacttggctg aagaaactat aaaagatgta ttgggagctg atgcatctat aggtatattt
     2281 aaaaattctg gtggagaaga tttccattat tatacacaaa aactaaaatg taaaactaca
     2341 tatattggat taggagctga tgctacacca ggatttcata atccaaatgt aacatttgat
     2401 acgaaagctt tagaatatgg tgtagatata tggtgtagat tggttgaaaa gagattggga
     2461 taacatccaa actaataatt ctacatttaa aattataatt atagaagtta tagaaaaaga
     2521 ttgcttatgt taattagaaa ataattaata tgagcaatct tattttgata taatgaaact
     2581 gattttggaa tattgaaaaa atatgatgga aaagtaatta tattttaaat ctattggaca
     2641 aaaaagtgat attattataa tattaagaat tgtaaaataa taccaaagtg catctagtaa
     2701 tagacttaac aattataaaa attttattct taggagggaa aaaatgagta agtataattt
     2761 tgacagagta atagatagag taggaacaga ttgtgtaaaa tgggatttta gaactaattg
     2821 ttcaacaaaa gcacaaaaag atggtttgcc tttttggatt gctgatatgg attttgaatg
     2881 tgcagaacca ataatagaag cattacgtaa aaaagttgaa cataaaatat ttggatatag
     2941 ttcaaatgat agtgataaat actttaatgc agtttgtgat tggtataaaa ggagatttaa
     3001 ctgggaaata aatagaaaag atattatttt tagtcctggt gttgtacctg ctgttgctat
     3061 tttagtaaga atattaacta attctaatga aggtgtaata gtccaaaaac cagtgtatta
     3121 cccatttgaa gctaagataa agagcaataa tagggacgtt gtaaacaatc ctctgatata
     3181 tgagaatggt acttatagga tggattatga tgatttagaa gaaaaagcta agtgtagcaa
     3241 caataaagta ctgatacttt gtagtcctca caaccctgtt ggaagagttt ggagagaaga
     3301 tgaattaaaa aaggttgtag aaatatgtaa aaaatatgac ctatggataa tagctgatga
     3361 gatacattct gatttaatta gaaaaggatt taaacatact cctttacagt cattatgccc
     3421 agagtacaag gataaaatag ttacatgtat agctccaagt aaaacattta atctggcagg
     3481 aatgcagttg tctaatataa tagtgaataa tgatgaacta aaaaagaaat atcaagaaga
     3541 ggttacagct gttggagttg caacttcacc aaatcccttt gctattgtag ctacaatagc
     3601 agcatataat gaaagtgaag actggcttaa tgaattaaat gattacttag ataataatat
     3661 acaatttatt gatgaatact taaaagaaaa cctaccaaaa gtaaaacttg tttatccaga
     3721 aggaacatat ctagcatggt tagattttac tgcttatgga ttaaatgaag tagaacttga
     3781 ggatttaatg tttaaaaaag ctaatgtatt atttgatgaa ggttatatat ttggaaaaga
     3841 aggaatagga tttgaaagaa taaatgtagc ttgtccacag tctttattaa aagaatgtat
     3901 ggacagatta aagactatat ttgaaaattt ataaagtata ggtaaaaatt tataaagtgt
     3961 ttaaaaataa aaaaataata aacttgacaa attttaacta atcacttatc atactattat
     4021 gaataaatag aaaacataag agagctaaat tttattgaat taaactctta aagactttga
     4081 aaggaagagt agttatagaa tgtaattaaa gcgagttggt gatggtgtga gaccaatatg
     4141 aagtctataa tgaagaacat cctggagttg ctaaccgaaa ttttaaaaga gtaggcttag
     4201 tcggaaccaa accgttatca tattggggtg tatcgagtaa tatctgtaca ttgtgagctg
     4261 ggtttgaatt tatttcaaac taatcagggt ggtaccgcgg aaaagcaacc tttcgtccct
     4321 tttatatagg gatgaagggt ttttttaatg gaaaaatata taaatatagg gagtgaatga
     4381 tatgagcaca ataataccaa aggattataa aagtagtttg aacgtaattg atacacaaat
     4441 agctataaaa aaattaaaag atttttttga aatgagacta agtaatgaat taaatcttat
     4501 aagagtttct tctccgttat ttgtattgcc agaaacaggt gcaaatgata atttaaatgg
     4561 agaaaaggct gtaagctttg atataccatt tatgaataaa aatgcagaaa ttgttcaatc
     4621 acttgcaaaa tggaaaagat tagcccttaa aaaatatggg tttgaagttg gtagtggttt
     4681 atatacagat atgaatgcaa taagaaaaaa tgaagaatta gacaatatac attctcttta
     4741 tgtagaccaa tgggattggg aattggttat agataaaagt agtagaaatg aaaagactct
     4801 aaaagatgtt gtaaaaagat tgtatggagt atttaaagat actgaaattt ttgtatgtag
     4861 tatgtatgaa ggtataaaag aaatattacc agaagaaata acttttataa catcacaaga
     4921 gttagaagat atgtatccag aattatcacc aaaggaaaga gaagataaaa ttgtaaaaga
     4981 gaagaaagca gtatttataa ctcaaatagg aaaaacatta atatcaggag aaaaacatga
     5041 tggaagaagc cctgattatg atgattggga attgaatggg gatatattat tttacaatcc
     5101 tgttttagat tcagcattag aattatcttc aatgggaatt agagttgatg aagaatctct
     5161 tgatagacaa ttgaaacttg ctggatgtga agagagaaaa gagtttgact atcataagat
     5221 gcttttaaat ggcgaacttc cgtatacaat tggtggagga attggtcagt caagaatatg
     5281 tatgtatttc ttacaaaaag ctcatatagg agaagtacaa gttggagtat ggccacagga
     5341 tatgattcaa aattgtagta gtgcaggaat tgaattatta taatttaaat ataaactaat
     5401 tgataaaatt tatatctaat atgttcatca aattaattat atcggttata atgtagaata
     5461 acctataatt ataagaggtg ataaatatgg agtttttaaa aataatcatt aatatagttt
     5521 tagatatttt aaaaaaaatt ttggtcagat ttaaaaatgc taaatttggt ttggtttttg
     5581 tatttgattt acttaaacta cctgatttta tgacagataa aagaatcaat atagttgata
     5641 aaataaaggt aatttctgta ttaattttta ctatatccta ctttgtatct ggggttgata
     5701 taattccaga gatgattgca ggggcatttg gttttataga tgatgctata gttttaattt
     5761 ggagtatagg tatagtgaat gaagaaatta ataagtatag agttatcgca aaaaaagata
     5821 aacattcaaa tataattgaa aatgtagaat tttctataaa agatgaagaa gaataacaat
     5881 ttatatatgt tattcttttt tttattaaca aaaaatggtt aaattgttga cggagaggta
     5941 aaactctatt atcataataa gtggatatcc aatatttaac taagtattca atgtgcaaga
     6001 atgcttaatt taaaaaagtt atggagggta aatattatga agaaaaaaat aggtttagtg
     6061 cccaaactaa tcatcggtat catagtcggt attttaatcg gaagttatgc accagaaata
     6121 atagtacaaa tattagtaac agttagtact ttattctcag cattcctaaa atttgttata
     6181 ccatttataa ttataggttt tgtaacagca ggtatagctg atttagcaac aggtgcagga
     6241 aagttattgg ggataactac gggaatagca tatggttcta ccttaatagc aggattatta
     6301 tcatttgcag tatctacatt aatcttccca aattttattg atgcaagtgt tgcaagtcaa
     6361 attggagacc cagaggcagg aatgttagct ccaatattca caattccatt agagcctatg
     6421 gtagatgtaa cagctgcaat cgtatttgca tttgtaatgg gacttggaat atcagcttta
     6481 agaaatcatg gaaaaggaga gactttattt aatttcttcc aagaatttca aggaatcgta
     6541 acgaaaacgt tgtcaacagt gattatacct ttattacctt tatacatagc aggaactttt
     6601 gcaaatataa ctatagcagg ggaagtatgg acaatattag gggtattctg gaaagtatac
     6661 ttagtagtaa tacctttaca ttttgtatat atggcatgtc aattcacagc agcaggtgta
     6721 ttttcaggaa aaaatccagt aagaatgtta aaaaatcaag tacctggata tttaacagct
     6781 ataggaactc aatcttcagc agcaactata ccagttaatg ttggttgtgc agaaaaaaat
     6841 ggagtaagta agcaaataag agaatttgtt gtacctcttt gtgcaacaat acatttatca
     6901 ggtagtatga tatcaataac aggatttaca actgcagttt taatgatgaa tggtatgcca
     6961 catggaatag gtgtattatt cccttatatt gcaatgttag gtatagcaat ggttgcagca
     7021 ccaggagcac caggtggggc tataatgagt gcacttcctt tcctaccaat ggttggaata
     7081 ccatcagatg gtgggcttgc aagtttaatg atagctttat acttaactca agacagtttt
     7141 ggtacagcat gtaatgtatc aggagataat gcaatagcag tagtagtaga taaaataaat
     7201 gatggaatga gtaaaaaagg aaataaagaa gagcaaagtg cttagttttt taataatcaa
     7261 aaatgagtta tctcaaatag agatagctca ttttttatgg tataaaaaat tataaaataa
     7321 agtgattttt ttatttaaaa attgctttaa gaaatgatat taataattta ttttcattag
     7381 gttttaatat acatactcta aagaaatact catctagacc ttcaaatgaa caacaatctc
     7441 ttattattat tttttgtggg agcagttgct ctcgaagaga tttagcagtc aattcctttg
     7501 tcttgatttt acacaatatg aaattacctt tagtattgta tatatctaag ctttttatat
     7561 tcttaagctc tttaagcagg taatttcttt cagtgttcat taaggaaata gtatttgata
     7621 taaaatctaa atcactaaac atgatttcac ccattttaga agctattatg tttatattcc
     7681 ataaatctag atttttattt atttcatttt tgacattagt gttagatata agaccatatc
     7741 caagccttat tccaggcgtt gaaaaaaatt ttgatgtacc tcttataacg aataaattag
     7801 aataatcatc aactaattga gtacaagagt atgtatctgt atcagtaaac tctacataag
     7861 tctcatcaat cattaaaaat gagtctgtat tttttaaaat ttctctcact tctactttag
     7921 taaatgcaaa tccagttgga ttactaggat tacagattac taccaaatca taatctttag
     7981 cattgatagt ttttataaga ttttctaaat ttatatgaaa attatcttct tcttttgcaa
     8041 agtatttttc tatagaacaa ttaattttag ataactcttt ttcatactct gaataggctg
     8101 gagagagtaa aagagcctgt ttagggttaa ttgtatgtat aaatgatgaa attagttctg
     8161 tagctccact gccaagtact atattatcta tagaacattt gcagtaattg gatatagaag
     8221 tttttaagtc tatgtaatca gggtcaggat acatagatac catatctata ttgtttacaa
     8281 tgtattgttt ggctaaactt gatgtaccaa aaggatttat atttgagcta aaatccataa
     8341 attcttcctt tgaaaagcca tacttactag ataaactata taaattagct ccatggtttg
     8401 tgctcataaa agtacctcca aaaataaaat tatataatcg ataaaaataa ctgattaata
     8461 tatgtacata tagattataa tacaaaggaa tcgttattcc aaatttttaa tggaaaaagc
     8521 aaaaaagtat ggataagctg ttaatttagg tttaaaaatg atgaaaaaga ttttaattga
     8581 tattttggta tgctataata tataagttaa tagaaggaaa gcgggaggat acaaaatgta
     8641 caaagttaca ggaaaaatgt tgacagaaga acaaataaaa gaaaaagttt atgaattagg
     8701 aaaaaagata gaagaagact ttaaaggcga agatttacta gtggtaggta tacttaaagg
     8761 agctagtgta tttgtttcag atttaataag atgtatagat ttggatgtaa atatagattt
     8821 tatgagtgtt acaagttatg gaaactcaac agagtcttct ggaacagtta aaatattaaa
     8881 agatttagat gtagatattg aaggaaagaa tgtgttgatt gtagaggata taatagattc
     8941 tggtttaaca ttaagtaatc tagttgcagc tttaaaaact agaaatccaa aatctttaaa
     9001 actatgtact ctacttgaca aacctcagag aagaaaagct aatatacctg tagactatgt
     9061 tggatttgtt atagaagaca aatttattgt tggttatgga atagactatg ctgaaaagta
     9121 tagaaattta ccttatatag gtatagttga ggatgtagaa tagatttaaa tgtgaatcaa
     9181 aagcactaat ttctaaggga gttagtgctt tttagttaat tatataaaat tttagtaaag
     9241 gagacatcta tatggatatt cagttaaata tattattatt tttccaaaat ttaagaaatc
     9301 caattttgaa ctttatattt cttgtcttta caataagcac agaagcaccg ttattaatat
     9361 taattacaac aattatatac tggtgtataa ataaaaaatg tggacagaaa atactgtttg
     9421 ctataattgg aaattttgtt gtaaatttag gcatcaaaga gtttgtaaaa gctcccagac
     9481 caataggaat aaaaggtctt gaatcattga gagtttctac tgctggtggt tattcttttc
     9541 caagtgctca tactcaaact gcaacaacat tttgggttag tattatgact atatttaaaa
     9601 agagatatct tcatttaata ggtgcagtta tggttttagg agtaggattg tctaggctgt
     9661 atttagctgt tcattggcca atagatgtga tagcgggttg gatattagga atatttttta
     9721 cagttgtatt tgtaaaaata tttgactata ttgatgataa taaaaattat attttacttg
     9781 tagttttact tataccattt ataatagtgg ctatatttct aaatagccct gaatattttg
     9841 aaaaatttgg tataatagta ggatttgtat ttggatatat ggttgaggat agatttgtta
     9901 aatttaatac agataataat aacaaaaaaa taaattttag taataaaaat aaggggagta
     9961 aaaatataat tttttcaaga atatgtaggt ttttggttgg gatagtaaca ataggaatgt
    10021 tatatattgg cattaagttg tttatttcaa tgttaatagt cactttaaat ataagtgact
    10081 ctactatgag tataattttt atgaatttta ttaagaatac agtagttgtt ttttatggaa
    10141 tagcaggagc tccagcatta tttaaacttt taaaattaaa ttgattttaa ggtatagagt
    10201 caatagttta aattaaaaaa attaagggta gaaaagagtg attatttgaa gaaggtagta
    10261 ataataggtg gtggaccagc agggatgata gccgcatcaa ctgcatgtga gaaaggctat
    10321 gatgtaacat taattgagaa aaatcataaa ttaggaaaaa aacttgcaat aacaggaaaa
    10381 ggtagatgta atattactaa cgcttgtgaa atagaggaat taatagaaaa tgtacctaca
    10441 aatggaaaat ttctttatag tgctttttat acttttacaa atgatgatgt aataagtatg
    10501 tttaataatc ttggagtaaa gactaaaaca gaaagaggaa aaagggtttt tccagagagt
    10561 gataaagcat ttgatattgt aaatgctctt gaaagacaac ttaaaagtaa gaaagtaaat
    10621 atattattaa attcgaaagt agaaaaaata atatctaaaa ataataaaat tgaaaaagta
    10681 attttaaacg ataaaaaaga aattaagtgt gattcagttg tagtagcaac tggtggatta
    10741 agttatccac ttactggttc tactggtgat ggatataaat ttgcaatttc acaggggcac
    10801 acaattatag atacaaaacc atctttaatt ggtatagaag tacaagaaag ttttacaaaa
    10861 gatttagaaa aattatcatt gagaaatgtt gaaataaggg tgtttaatag caaacaaaag
    10921 aaagtttata gtgattttgg agaacttgaa tttactaaat ttggtttaga tggacctata
    10981 atcaagtctg caagttgtag aatgaaagat acaaggaaag aaaattacac tatattatta
    11041 gatttaaaac ctgcattaga tgaggaaaaa ttggataaaa gagttcaaaa agattttcag
    11101 aagtatacaa ataaaaaatt tgaaaaagca ttagatgatt tacttccaaa aaaactaata
    11161 cctataataa taaacttaag tgaaattaat ccaaatacgg ttgttcacca aatttctaga
    11221 gagcagagaa aaaacttagt ccatttatta aaaaatttaa agtttacagt aaagagatac
    11281 agacctattg aagaagctat aataacttct ggtggtgtaa aagtaaacga aataaattca
    11341 agtacaatgg aatcaaaatt agtagaaggt ttattttttg ctggagaagt aatagatata
    11401 gatgcatata caggtggatt caatttgcaa atagcttttt ctactggata tttggcaggt
    11461 tttaattgct aaaaattacc tgaattaaaa atgtttaaaa tcacataata ttatcagcaa
    11521 cacaaaataa attaaacaga aggaaggtaa tcaacatggc aagtaacaat aacaacaaca
    11581 gaacagtagt accaggagca aaagaggctt taaaccaaat gaaattagaa atagctaatg
    11641 aacttggtat gagtaattat caacaagttg ataaaggaaa ccttactgca agagaaaatg
    11701 gttatgtagg tggatacatg actaaaaaat tagttgaaat ggctgaacaa caaatggctg
    11761 gaaaaagcaa cagatagtta taaataatta tttataacta ttggctatta gtttagctag
    11821 agctatctca aatatagaaa gtaatttcta aattgagata gctttttttt attaaattat
    11881 taaatagata tatttttatg taataaattt tatgtatttg gataaaataa tacaaatgaa
    11941 tatgttacat aaggagtgat gtaaaatgag tagtgtaaat tttgaggatt taaaaaacaa
    12001 atttattaat agtgacttag atgaaaaaat aagaatatat acaacaacag aaggtttatc
    12061 agtagaacaa tttaaagagc tactaaaata ttatccaata caacatttat ctaaactaga
    12121 aaaagcactt ggatagcagt atttaatcca aaattaaaaa ggcaatttat ttaatattaa
    12181 aaatattttt tattaaataa attgcctttt tataataggt ttaaattatt atgcttttga
    12241 tatataatta tagattctct catttccaag ttcaatagac ccagtattaa taagagtaac
    12301 tattccttct atatctctag cagttaagta tttatatgga attaataaat tataaccagt
    12361 attacttagt aagtttttta attgggttat ttgagagata ttgaaacatt ttgcagtttt
    12421 agtagatttt tttcttaagt tagataaatt ttcattttga atatttatta aagtttctat
    12481 atagtttcta taagatatct taaagtttgt ctcattgact attttttcat taagtatact
    12541 actgataaaa tcttgagaca atatattatc tgcaaattta tctgctgtta attttccaga
    12601 tgttatgcct ttttgatatt ttctaagaat ttttttagta tagcagtata ttttatttct
    12661 cgtttctaaa tcgagttgtt ctatttgcac aaaaccactt ccttctaatt ataaaaatat
    12721 ttattaaaaa tctatactta actaataaag cttcaataac ttatttacaa taaatactaa
    12781 atcataaaat atttgttatt ttttattatt accaagtata aaaaatatta taattgtaaa
    12841 acttacaaat atatgtaaaa tatttaggag tgatgaaaat gttaaatggg ataataaagc
    12901 ttttttattc tgaaattgta aaaacaaaga ttaataactt agattcaagt ggatattctt
    12961 taagagatga atgtaaagaa atcagaaata taactctaaa gatagatagt gtatttttta
    13021 agcttttaaa gttagctttt ggttcaatat tgcttatggt agcttttaat ttaaacatgg
    13081 ttttaggtat aggagtgttt ttagttggtg tagtttatat actatataaa agaaatatgg
    13141 aagaacaagt aaaagagcat ataaataatg tgaaaaataa tatcgacatc tctaaaataa
    13201 cttttatgga tgaaaaaggg agaacaggtc taaatgcatt aattacatta cttcttatag
    13261 gattattgac aggatttaac tggatattag ttgctagttt tgtcgttgtt tttgttttta
    13321 ctataaagaa tgtatgttaa aatactaata aatagtttaa tatatagggg gagaaattga
    13381 gtatgactaa tggcagttat aaaatgaaga atttagtgga aatatccgct atggtgactg
    13441 aatctacaga tttttttaat ataaaagatg acataataga aaaaatgtta gaagttgttc
    13501 accctacaaa ggcatgtgtg aatctatttt ataaaaatga ttctaaatat gcctacttag
    13561 tatgttcgca aaccttagaa tatgtacctc aaatttttcc aataaatagt cttaaaggtg
    13621 caaaaattga ttttgacaca tatccagagt atatacatga ggctgtaaat gaaaaaaaaa
    13681 taatatatgt ggaaaatgta tttgaagatt ctagagcaga aggtgaacga gatttagcaa
    13741 aagctgaagg ttatattggg agaatagttt ttccttttat aataaataac gtagttgttg
    13801 gatttatgac ttgtttttta aaagaggatg attatataac agaacaagat atagatttta
    13861 tatcatctgt tgcatcactc ttaagtttat caatagaaat aactaataaa aataataatg
    13921 tacaaatttt aattgataaa ctaagaggct caataagtgc cataaatgaa gctactaaaa
    13981 aactatacct taataagagt attgatggat ttttggaaaa tttaagtaaa caagcgtgta
    14041 atattactaa tagtaaagaa gctttaataa ttataaatag tcatcataat aaggatgcta
    14101 tagttagttg ttataatgtt gagcaaaaaa agaaaacaaa tttatatcct atgattgata
    14161 tgtttttaaa gaaagagtca ttgggggggt atttaaataa tttaaaacca tgctcaaaaa
    14221 agggaataaa tttggaaagt tatatatatt acaaacttca ggataaaaat gagactatag
    14281 gatgtatagt ttgtgcaaat agcaaaaact atactagtga tgatttaaat attcttagta
    14341 tattgtccaa acaagtttct gttggtatgc aattatatga acataatgaa aaggaagtaa
    14401 aacacaaagt tcttgaaaac gagttgaaca tattaaataa acaacaacag cttattatgg
    14461 ataaaggtaa aatggattgt aatgatgaga aagaacttta tttttaccat aaaccagcaa
    14521 gggtagtagg tggagatttt tattatgcta caaaagtgga tgatgaacat atagtttata
    14581 ttgttgctga tgtaatggga catggaatgg tatcaaatta tgttgttgca atgataaaag
    14641 gtgcttttaa agtattatgc aatcaataca atacaccaag agaaataatg acaaatttaa
    14701 ataggatgtt atatgatgaa tttgacaaga tgggagtatt tacaacttgt ttgataggta
    14761 ttatagatac taaagaaaat atgattactg tgtctaatgc aggtcactat agtcctgtag
    14821 ttgtaaagaa agatggcaca atagaaaata atttaaattg taaaaagggc atacctattg
    14881 gaataatgga agatgctact tatgaaaata atactctttc aatggaagat tatgcaatgg
    14941 tatgtatgta cactgatgga attttagaga taaaaaattc atcaaaagaa gagtatggta
    15001 taaacagatt agaaaacttt ctaaaagaaa attttagatt aaatcaacag ttaatagtag
    15061 aaaatctaaa attggattta aagaacttct catcaaaaga taattatgat gatgatattt
    15121 taatagttat gttaaaggat aggtagattg attatggatt caaacgataa aaaaggactt
    15181 aaaagtccag agattataat aataaatgga agtccaagaa ttaataaaaa ttgcagtttt
    15241 atagcaaatg aagtaaaagc tatgctagaa gagtgtggat taagttgtaa aatatttaat
    15301 atctacaata tggatattga atattgcaca gcatgtggat tttgtgaaaa aacagggtat
    15361 tgcaagttta atgatgatat gaagccatta tataaaatgt ttgatgattc aaaaggaact
    15421 atagtaataa gtcctgtaca ttttgattca atatcagcta aattaaagac tgttgttgat
    15481 agaactcaag ctatatatgc aagtaaatat gtattaaata aaccttcaat tgatagaagt
    15541 aagaaacgga taggaatgta tattgcagtg ggagggagta aatcatatga ttcacagttt
    15601 aaaggtggac agattgttat ggattttttc tttaagagta taaatactaa acttttacac
    15661 aatatatatt taaacaatac agatgatgta tcttatatag ataatcaaga atttaaagat
    15721 ataatatata aaaatttaaa gatatatatt gattcaatat aatttgattt tgtaggtaat
    15781 attatgtata attgtattgc aataatttaa ataattaaaa ggagggaatt tcaatgaaat
    15841 taccaatagc attatctaat aaacatgttc atttgagtca agctgatata gacgtattat
    15901 ttggggcagg tcatgagtta actaaattta aaccattatc tcaaccaggg caatatgcct
    15961 gtgaagaaaa agtggattta gtaggaccaa aaggaacttt aaaaggagtt agagtactag
    16021 gaccagctag agctcatact caagttgaaa tatctttagc agatggtttc ccattaggag
    16081 taaaagcacc aataaaagaa tctggtgata tagctggaac tccaggtgtt aaattagtag
    16141 gacctgctgg agaagttgaa atgaaagaag gagttatagt tgcttctaga catatacata
    16201 tgagcaatga agatgctgct aaatttggtg taactgataa gcaaatagta aaagttaaaa
    16261 catctggacc aagagcatta gtatttgaaa atgtattagt aagagctagt gacagcttta
    16321 atctagaaat gcatgttgat atggaagaag gaaatgcagc aggcgttaaa aatggagatt
    16381 tagtagaatt aatagttgat taatattata aatagacttt atgctagtag atattttatt
    16441 tgctagcata tttttaaatg taataaattt ctataggata aatttaatcg attagattaa
    16501 tttataaaat aaaggggtat tataaaaaat gtaataaaaa c
//
LOCUS       DBGJFF010000083        16387 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.084, whole genome shotgun sequence.
ACCESSION   DBGJFF010000083 DBGJFF010000000
VERSION     DBGJFF010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16387
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.084"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(60..1148)
                     /locus_tag="ACOIAF_002613"
     CDS             complement(60..1148)
                     /locus_tag="ACOIAF_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669617.1"
                     /translation="MNFNFAKATNSRLMGSLGLIINWIDDENNHFCQYFLLDAEGLGL
                     ADYVSLNNPTQEEAYMEEERLMGGFGSDRVELTKDESLFLVSYFGNKNLYYDKLLPGD
                     KCEYIDIIKNYKTDLTIEKLYNKICKRVDEEVEFINYMTMRFIAWDRESLKYFSGSDE
                     IANMHITNINGTLLKNVVSDKGQGRYISEALYEDNDGYYICKIAFCISKCYETGFRIN
                     SLLVTDKEPMYDFEVFDEISKSEFVSVYSVNSPEEFAGVFYRENPFLLKSNMNEGIFF
                     TRFNFNNDHVKENVYIINNDMKAIYYLMGNKFFIGTYNENDCNYINEISQSNYSDYIK
                     FEEKFFFEQNALYDFAESGSLDFDDFLE"
     gene            1273..2001
                     /locus_tag="ACOIAF_002614"
     CDS             1273..2001
                     /locus_tag="ACOIAF_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HGZ2669618.1"
                     /translation="MKNIAAFFDIDGTLYRDSLMVEHFKKLIKYDIIDQKNWYKHARD
                     TFMDWDKRQGNYDDYLLEICDLYVDSLKGLDKTCINFTSDQVIKLKADRVYKYTRSRI
                     EWHLNQGHIVIFISGSPGFLVEKMAKKYNVTDFLGSDYVFENNIFTGTVIPMWDSISK
                     NNAINDFVVKYDLDLSNSYAYGDTNGDINMLKRVGNPIAINPTKELLSQISLDEEISK
                     NVEIIVERKDIIYSLSTDVNIVDI"
     gene            complement(2202..2693)
                     /locus_tag="ACOIAF_002615"
     CDS             complement(2202..2693)
                     /locus_tag="ACOIAF_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669619.1"
                     /translation="MSIKVAYGGVLLALNVILLTLTNIIPVNTLFIMGLASLLVSIVI
                     MEWGFKSGIAFYIGSIVLGFIVMASKSQWIVYSLTFGIYGIVKYLIEKDRSIYIEYFM
                     KLVFANIMILILYFLLRTIVYIPINIFIIGSFEIAFIVYDYVYSSFIGYYNNRLRKML
                     FKK"
     gene            complement(2704..3147)
                     /locus_tag="ACOIAF_002616"
     CDS             complement(2704..3147)
                     /locus_tag="ACOIAF_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669620.1"
                     /translation="MYSNTYNNENFMRARAFIENSEFKKAYDFLKTLTDKCAEWYYLT
                     GFSAMNIGYYEEGEDFLKRAKFMEPENSEYSDALRSYTQYRNDYSNRADNYNRRRRND
                     LDGCCCCCCDDCCCCLGDDCCENCAKLWCLDSCCECFGGDLITCC"
     gene            complement(3137..3976)
                     /locus_tag="ACOIAF_002617"
     CDS             complement(3137..3976)
                     /locus_tag="ACOIAF_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5685 family protein"
                     /protein_id="HGZ2669621.1"
                     /translation="MFGYVKINKMDLTFREYDYYKAYYCGLCKYLKRNHGEISRFSLN
                     YDITFLIVLLTAVYNPESISTEEVCIVNPFKKKKVITNDITEYAASMNILLTYYKLED
                     NLMDDKRIKDKLAYYIYKNKLKLAYEKYPEKAEYIKQQLNELNKLEKDKNINIDEVSS
                     IFGNIMGEVFVYKKDENERNLRMIGFNIGKYIYLLDAYEDLDEDFKKGRYNPFIEYID
                     KNDELKEKVKKIIVTSLGFLARGIDNLNLNKNVGIIENIIYSGVYLRYINILESRGGK
                     NVQ"
     gene            complement(4195..5580)
                     /locus_tag="ACOIAF_002618"
     CDS             complement(4195..5580)
                     /locus_tag="ACOIAF_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HGZ2669622.1"
                     /translation="MENLNKKFLMEYIDEIQGELRDISDKIWKNPELQYKEYYASNLQ
                     KECLKKHGFSIEGLEGMDTAFVASYGEGRPVIAVLGEYDALDGLSQKVSTTKEPIVDG
                     GAGHGCGHNLLGTGSIGAVIAVKELIKLKKLKGTIKYYGCPAEEDLSGKVLMIKKGFF
                     DGIDCAFSWHPFDINTPIRIPTLANYSVKFRYNGISAHAAQAPYNGRSALDAVELMNI
                     GCNYLREHIFDSCRVHYVTTNGGKMPNIVPDFAEVWYYIRGVKMEHVRDVFGRIVDIA
                     KGAALMTGTIMEYDIISGVYDYIPNTILTDILSQNMKLVGVQNYNENDYYFADKLAET
                     VSIDKRSSVSSVLSGNKDITKMNLHDEVTDDTFTHNNCISLSLDIGDVSYIIPTAQCS
                     CSVWPIGISAHTWQSCASAGSDMGFKAMLLASKSISCSINDVMLDELVINKAKKELKY
                     TVGSFKYIPII"
     gene            complement(5752..6495)
                     /locus_tag="ACOIAF_002619"
     CDS             complement(5752..6495)
                     /locus_tag="ACOIAF_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HGZ2669623.1"
                     /translation="MKKVLIVGESWVKNITHIKGFDTFVTTHYEEAVKWLKEAIESGG
                     YETVHMPAHVAADSFPYKLEELNEYDCIILSDIGSNTFLLSNSTFIDCNSNPDRLELI
                     KEYVNNGGALIMVGGYMSFTGIDAKARFGETAIKDVLPITMIDKDDRVEKPAGIIPEV
                     IDSEHPVLKGIPTEWPKFLGYNKTVARDNCPVLATIGGDPFVAVGEFGKGKSAIFSSD
                     CAPHWGPKEFTDWKYYNKLWVNMLDWLTC"
     gene            complement(6515..7777)
                     /locus_tag="ACOIAF_002620"
     CDS             complement(6515..7777)
                     /locus_tag="ACOIAF_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HGZ2669624.1"
                     /translation="MKKILLDTLNSKKQEYIDYLKELVSIKTEDVGHGILGGFEKEGQ
                     EYIEKLANYIGFSVDRQEMSEELIKKAKNIYKEGNLGHNYQDRYNLICKYSDDLPGKT
                     IVFNGHVDTMPPGDISKWKYNPYRATEDNGKLYGLGTADMKSGLIASILAVKLIKDSG
                     LNVPGNVKIMSVVDEEGGGNGTINAVMNGIGGDCCIICEPSEQNLIVAHMGFVFFEVE
                     VKGVSLHCGSKWEGVNAIEKAMLLLQDIKELEHNWLMIYKHPLLPSPTINLGVINGGT
                     AGSTVPDKCVFNLCVHFLPNIMSYEQVVNDVTNVIMTRANGDLWLKDNKPNINIYQSG
                     LGFEMDKDSDFVVNAHEILEETLGKKLEIKGSTAGNDARLMKNLAEIPTLILGPGSIE
                     QCHSIDEYVEIKEYLDSILMYASLILNL"
     gene            complement(7961..8959)
                     /locus_tag="ACOIAF_002621"
     CDS             complement(7961..8959)
                     /locus_tag="ACOIAF_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2669625.1"
                     /translation="MSSITKHKEEKSSPLLTKLTSVFRDQGSAAVGLVIIFIIMSVAS
                     SNFLTLDNLINVGRQISINAILAVGMTFVIITGGIDLSVGAVIALVGTFWATTVVNYN
                     APIWVGMILALAIGTILGVIKGAIISTQKLPPFIVTLAMLTIISGASFVFTGGRPISV
                     NTDAFKMLGRGYIGPIPIPVIIMIIVVIAGHFLLKRTNFGRHVHAVGGNEEAARLCGV
                     KVNKVIVKVYALAGLLTALAGIILSSRLASGSPTVGDGAELDAIAAVVLGGTNMMGGS
                     GSIVGTCIGVGIIGILGNGLNLLSVSSYNQMIIKGLVMLFAIWINNIKLKKSAKSK"
     gene            complement(8943..10454)
                     /locus_tag="ACOIAF_002622"
     CDS             complement(8943..10454)
                     /locus_tag="ACOIAF_002622"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HGZ2669626.1"
                     /translation="MSNIILKLSNIAKEFPGVRALDNVNFELFHGEVHALLGENGAGK
                     STMIKILTGAHSKTSGKFIFEGKEIENISPDISKKIGINAIYQELTVFDELTVAQNIF
                     MGKEINGKVLTNDKKMNEEAKKIFDNMGIDINPNSLVKELSIAQKQMVEIARVLSSET
                     KVLIMDEPTSSISKKETEILFRLINDLKESGVSIIYISHRMEELFEICDRITIMRDGK
                     TISTLNTKDVSSEEELVNLMIDRKLDQFFPKRKVEIKEEIMRVENLTKNNVFNDISFN
                     IRKGEILGIGGLVGSKRSEIVEAIFGLRTYDSGKIYLNNEEVKFKTPSDAIENGLGLI
                     TEDRKGTGLFLQMSVKENTTMAGLKKISKFKSIIDRKKEKEILEKYIEALKIKTPHMN
                     QVIQSLSGGNQQKAIIARWLLLQPDILIMDEPTRGIDVNAKAEIYNLMGDLVESGVSI
                     IMISSEIPELISMSDRIMVMREGHISGFLEGEEMVENNVLKLAFGGKINEFNN"
     gene            complement(10508..11470)
                     /locus_tag="ACOIAF_002623"
     CDS             complement(10508..11470)
                     /locus_tag="ACOIAF_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HGZ2669627.1"
                     /translation="MRVRKILSLGIAGILAIGMLTGCSMEGPSKSDNKGGSDKKDLTI
                     GVSTITLQHQFFIDIDEGIKEKAKELGVKVIVNDPDQDVAKQTSAIEDFIQQNVDGMI
                     VLGTDNSAIVPAVEGAFEKMPVVTVDAVLNTENITSYVGTVSYDAGKKLGEYTKKYID
                     EKLGGKSEIAIVTDLKSQIQMQRIDGFKDALKGSANVKILNSQPGYDREESLNTVENL
                     IQSNPDVDIIYATAENSVLGAKAALESAKNKDVKIVGFDLTEEASSGITDGTILAMIQ
                     QQPKEMGRLAVEAVVKAIKGEKVEKNIPVPALLYDKENIKDFKN"
     gene            complement(11518..12408)
                     /gene="rbsK"
                     /locus_tag="ACOIAF_002624"
     CDS             complement(11518..12408)
                     /gene="rbsK"
                     /locus_tag="ACOIAF_002624"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HGZ2669628.1"
                     /translation="MGNIVVIGSVNMDMVCSVDKRPEKGETVLGNSFFTSPGGKGANQ
                     AISASKLGANVKMISCIGEDGLGEELIRNFRTNKVDYSLVSRNNHKSSGVAVITLCEN
                     DNSIVVVPGTNELVDIELIKKNEEEIKNADIVLLQLEIPLKTINYVVNFCFENRIRVL
                     LNPAPAVKLNEDIIEKVTYLTPNEHEYKIVFDTNEGIEEVLKKYPNKLVITEGKNGAR
                     FYDGEEIKHVSCISVDVQDTTGAGDTFNGALAVAITEGKNLYTAVEYAVVVSGLSVTK
                     LGAQSGMPYKEDVEKYLNNK"
     gene            complement(12450..13472)
                     /locus_tag="ACOIAF_002625"
     CDS             complement(12450..13472)
                     /locus_tag="ACOIAF_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGZ2669629.1"
                     /translation="MNFLSITIKEIGELAGVSKTTVSKVINNKDENISQATREKILKI
                     MKEKNYVPNKLAQSLVTKKTNTIGLLIPDIRNPFFTDVSRGVEDKANEEGYNIILCNT
                     DEDAKKEYEGIRTLSERMIDGIIFAASSNTNWKEANYKDIKIPTVLIDKKISMNKETL
                     KGIVKINNFEGAYIATKHLLDIGNKKIIYLSGPLQNEIAVDRLEGYKKALIECNLSYN
                     PAYVFEGKYKIEWGQEFIKNLEKIDFDAIFCANDLIAIGVIRGLKERGLSIPNDISVV
                     GFDDIQTSSLISPSLTTVKQPSYDIGYKASEILINCLRGDKKESFDELIFKPELVIRD
                     STKESE"
     gene            complement(13692..14669)
                     /gene="bioB"
                     /locus_tag="ACOIAF_002626"
     CDS             complement(13692..14669)
                     /gene="bioB"
                     /locus_tag="ACOIAF_002626"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HGZ2669630.1"
                     /translation="MEKYIIKLKNKVLRGKEISYEEALNLISLDTNNKNDFDTLLKSA
                     NEIREYFMGRKADLCTIMNAKSGKCSEDCKFCAQSSHYKTGVEEYSLLDYNEILNRAK
                     EMESKGVHRFSLVTSGKGMSGKEFNDILNIYEGLRKNTNLKLCASLGIIDYEKAKMLK
                     SAGVTTYHHNVETCRDNFHNICTTHTYKDRIKTIKDAKKAGLDVCVGGIIGMNESEEQ
                     RLKMAFEIRELNVKSFPINILNPIKNTPMENYDVLEPMEILKTTAVFRFIIPNVYIRY
                     AGGRLSLKGYDKVGFNGGVNSAIVGDYLTTVGSGIEDDKKMIIEQGFEL"
     gene            complement(14875..15138)
                     /locus_tag="ACOIAF_002627"
     CDS             complement(14875..15138)
                     /locus_tag="ACOIAF_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669631.1"
                     /translation="MKIYYEKENSKKANVNNIPSVFAFNFLRNFLTSKEYEETRKEYF
                     INNLTKSQVNQAMKDLKWLFREYEGLEVITMESLDGVKTRIVL"
     gene            complement(15208..16281)
                     /locus_tag="ACOIAF_002628"
     CDS             complement(15208..16281)
                     /locus_tag="ACOIAF_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HGZ2669632.1"
                     /translation="MEKSKVYFCDLYSNSQNKNVPNNVRRLFDEAGFKDLIEKNDQVA
                     IKLHFGEKGNTTYMSPVAVRQVVDKVKDCEGKPFLTDTNTLYTGSRTNSVDHLTTAIE
                     NGFAYAVVNAPVIIADGLYSRNYENVKIDKKHFESVKIGGEIYNSSAMIVMSHFKGHE
                     AAGFGGALKNLAMGCASAAGKQMQHSDVTPVVKEKKCVGCGKCVNSCPTKAISIVDKK
                     AVIDSDVCYGCGECPTVCPTRAVTIQWESDSDVFVEKMAEYAYGAVSNKKDKVGYITF
                     VMNVTPLCDCVPWSGRPIAHDIGILASTDPVAIEQACYDLICKEMGHDVFKHEHPHVN
                     GTRIIDYACEMGMGSKEYELIKL"
BASE COUNT         5150 a         3051 c         1642 g         6544 t
ORIGIN      
        1 gaaataaatt taagcgagat taaattcagt taatctcgct taaataatgt ctaaatttat
       61 tattccaaaa aatcatcaaa atctaatgaa cctgattctg caaaatcata taatgcattt
      121 tgctcaaaga aaaatttctc ttcaaattta atatagtcgc tataattaga ctgacttatt
      181 tcattgatat aattgcaatc attttcgtta taagtaccaa taaaaaactt atttcccatt
      241 aagtaatata tcgctttcat atcattattt attatatata cattttcttt tacatggtca
      301 ttattaaaat taaatctagt aaaaaatatg ccttcattca tattactttt taacagaaat
      361 ggattttctc tataaaatac tcctgcaaat tcttcaggtg aatttacact gtaaacactt
      421 acaaattcac ttttagaaat ctcatcaaat acctcaaaat catacatagg ttctttatct
      481 gtcactaata gagaattaat tctaaaacca gtttcataac acttacttat gcaaaaagca
      541 attttacata tataatatcc atcattgtct tcatataaag cttcacttat atatctgcct
      601 tgacctttat cacttaccac gtttttaagt aatgttccat ttatattagt aatatgcata
      661 tttgcaatct cgtcacttcc agaaaaatac tttaaacttt ctctatccca tgctataaat
      721 ctcatggtca tatagtttat aaattcaact tcctcatcaa ctcttttaca tattttattg
      781 tataattttt ctatagttaa atctgtttta tagtttttta taatatcaat atactcacat
      841 ttatctcctg gcaatagctt atcataataa agattcttat taccaaaata agatactaaa
      901 aataaagatt catccttcgt taattcaact ctatctgaac caaatcctcc catcaatctt
      961 tcttcttcca tataagcttc ttcttgcgtt gggttattta aactcacata atctgctaat
     1021 cctaatcctt ctgcatctaa taaaaaatat tgacaaaagt gattgttttc atcatctatc
     1081 caatttatta taagacctaa actgcccata agtctagaat ttgtagcctt tgcaaaatta
     1141 aaattcatat agtcacatcc tttaaactct aaaatacttg ttttttaata tataaattat
     1201 tatacatata ttttaacaac ttagatacaa tttataatat aatataagga aatgattaat
     1261 tgaggtggtc ttatgaaaaa tatagcagct ttttttgata tagatggtac tctttataga
     1321 gactctctta tggttgaaca ttttaaaaaa ttaattaaat acgatataat tgaccaaaaa
     1381 aattggtata aacacgctag agacactttt atggattggg ataaaagaca aggtaattat
     1441 gatgattatc tattagaaat atgtgacctt tatgttgatt cattaaaagg attagataaa
     1501 acctgtatca actttacaag tgaccaagta attaaattaa aagcagatag agtttataaa
     1561 tacacacgtt caaggattga gtggcatcta aatcaaggac acatagttat atttatatct
     1621 ggtagccctg gatttcttgt tgaaaaaatg gcaaaaaaat ataatgtaac cgattttcta
     1681 ggaagtgatt atgtctttga aaataatata tttacaggca cagttattcc aatgtgggat
     1741 tctataagta agaataatgc tataaacgat tttgttgtaa agtatgattt agatttatca
     1801 aactcgtatg cctatggaga tactaatggt gatataaata tgctaaaaag agttggaaat
     1861 ccaatagcaa taaatccaac aaaggaactt ttatctcaaa tatcacttga tgaggaaatt
     1921 tctaaaaatg ttgagataat agtagaaaga aaagatatta tttattctct atctacagat
     1981 gtaaatatag tggatattta atttatacta tctattttaa aaacttatat tatatctaat
     2041 atttatatct aacatataaa agctttgact aaaaatcaaa gcttttatat gttgcactaa
     2101 gaatatatta aatttagctt tatgttttaa tgtatattca tatatataat ttaccttatc
     2161 caaactacaa tttcattaaa tttaaagata aaaataagca attatttttt aaatagcatc
     2221 tttcttaacc tattattgta ataacctata aaagaactgt aaacatagtc atatactata
     2281 aaggctatct caaatgaacc tataataaat atgtttattg gaatataaac tattgttctt
     2341 aataaaaaat acaatataag tatcattata tttgcaaata ctaatttcat aaaatactca
     2401 atatatatag acctatcttt ttctataaga tattttacaa taccatatat tccaaaggta
     2461 agtgaatata caatccattg agacttactt gccatcacta taaatcctaa aactatagaa
     2521 cctatataaa aagcaattcc actcttaaag ccccattcca ttattacaat tgatactaaa
     2581 agtgatgcta accccataat aaacaatgta tttacaggaa ttatatttgt aagagttaac
     2641 aatattacat ttaatgctaa taaaactcct ccataagcaa cttttatact catatttatc
     2701 atcctaacaa caagttatta aatctccacc aaaacattca caacatgaat ctaaacacca
     2761 aagtttagca caattttcac aacaatcgtc acctagacaa caacagcaat catcacagca
     2821 acagcaacaa catccatcta aatcattacg tcttcttcta ttataattgt cagccctatt
     2881 gctataatca tttctatatt gtgtataact tctaagtgca tcactatatt cactattttc
     2941 aggctccata aattttgctc tttttaagaa gtcttcacct tcttcataat atcctatgtt
     3001 cattgcagaa aagccagtta aataatacca ctctgcacat ttgtcagtca aagtttttaa
     3061 aaaatcatat gcttttttaa attcactatt ttctatgaat gctcttgctc tcataaaatt
     3121 ttcattatta taagtattac tgtacattct ttccacctct actttctaat atattaatat
     3181 atcttaaata aacaccagaa taaattatat tttctattat tccaacattt ttatttagat
     3241 ttagattatc aattccccta gctaaaaatc ctaatgatgt tactattatt ttttttactt
     3301 tttcttttaa ctcatcattt ttatcaatat actctataaa tggattatat cttcctttct
     3361 taaaatcttc atctaaatct tcataggcat ctagtagata tatatactta cctatattaa
     3421 aacctatcat tcttaagttt ctctcatttt catctttttt atagacaaat acttcaccca
     3481 ttatatttcc aaatatacta gatacttcat ctatatttat atttttatct ttttctaact
     3541 tatttaattc atttagttgt tgttttatat actctgcttt ttcaggatac ttttcatatg
     3601 ctaattttaa tttgttttta tatatataat atgccaattt atcttttatg cgtttatcat
     3661 ccattaaatt atcttccaat ttataatacg ttaaaagaat attcatactt gctgcatatt
     3721 ctgtaatatc atttgtaatt acctttttct ttttaaatgg atttactata cacacttcct
     3781 ctgtacttat agactctggg ttatatactg ctgttaataa aacaattaaa aatgttatat
     3841 catagttcaa agaaaaccta gagatttctc catgatttct ttttaagtat ttacatagtc
     3901 cacaatagta agctttgtaa taatcatatt ctctaaatgt taaatccatt ttgtttattt
     3961 taacatatcc aaacataaat actccttttt gtactatttt ttatttagat tatatatagt
     4021 ataacacttt ttatcaagat tatataattt acagtattct aagaaactta aaaaattcct
     4081 tctaatcatt gtatcttaaa tttaaaataa ttagaaggtt gggttaaaaa taatattaaa
     4141 ttatatactt atattaactc atacaagtta gttgtgctta tatcaattta agttttaaat
     4201 aattggtata tatttaaaac tacctacagt atacttcaat tctttttttg ctttatttat
     4261 aaccaattca tcaagcatta catcatttat tgaacatgat atagatttag atgccaataa
     4321 cattgcttta aatcccatat cagaacctgc actggcacaa gattgccatg tatgggcaga
     4381 aataccaata ggccaaactg aacaagagca ttgtgctgta ggaattatgt agctaacatc
     4441 tccaatatcc aatgataatg atatacagtt attatgagta aatgtatcat ctgtaacctc
     4501 atcatgtaaa ttcatcttag taatatcctt atttccactt aatacactag aaacactact
     4561 tcttttatct atagacactg tctcagctaa tttatctgca aagtagtagt cattttcatt
     4621 atagttttgc acacctacta atttcatatt ttgtgataga atatctgtta atattgtatt
     4681 tggaatatag tcatatactc cacttataat gtcatattcc ataatggtac cagtcattaa
     4741 tgctgctcct tttgctatat caacaatcct accaaataca tctcttacat gctccatttt
     4801 aactcctctt atgtaatacc acacttctgc aaaatcagga actatatttg gcatttttcc
     4861 accattagta gttacatagt gtactctaca actatcaaat atatgttctc tcaaataatt
     4921 acaacctata ttcataagtt ctactgcatc aagtgcactt cttccattat agggagcttg
     4981 tgctgcatga gcactaatcc cattatatct aaatttcaca gagtaattag caagtgttgg
     5041 gattcttatc ggagtattta tatcaaatgg atgccaacta aaagcacaat caattccatc
     5101 aaaaaaacct ttttttatca tcaatacttt tccagataaa tcttcctctg ctggacatcc
     5161 atagtatttt atagtccctt tcagcttttt tagtttgatt aattccttta ctgcaattac
     5221 agctcctatt gaacctgtac caagtaagtt atgtccacat ccatgacctg caccaccatc
     5281 tacaataggt tctttagtag tggatacttt ttgagacaaa ccatctaaag catcatattc
     5341 tcccaaaact gctataactg gtctaccttc tccataacta gccacaaatg ctgtatccat
     5401 accttctaat ccctctatac taaaaccatg tttttttaaa cattcttttt gtaaatttga
     5461 agcatagtat tctttatact gaagttctgg atttttccat atcttatctg atatatctct
     5521 taattctcct tgtatctcat ctatatattc catcaaaaac tttttattta aattctccat
     5581 attaattata taaacctccc tgtttctaat aacttataga ttatcagcca aaatgactaa
     5641 ttttaactga tttaaaaata ttttctacca taaaccattt tatacaataa aacctcttga
     5701 cttgtaagtt tcattttatc aagtaagagg ttttcaagtt ttataaaatt tttagcatgt
     5761 taaccaatca agcatattaa cccataattt attatagtat ttccaatcag taaattcttt
     5821 tggaccccag tgaggtgcac agtcagaact aaagatagca gattttcctt ttccaaactc
     5881 accaacagct acaaatgggt ctcctccaat agttgctaaa acaggacaat tatctctagc
     5941 tactgttttg ttatatccta aaaactttgg ccattctgtt ggtatacctt ttaatactgg
     6001 atgttcagag tcaataactt ctggaattat tcctgctggt ttttcaactc tatcatcttt
     6061 atctatcatt gttattggta atacatcttt gatagcagtt tctccgaatc tagcttttgc
     6121 atctattcct gtaaatgaca tatatccacc taccatgatt aacgccccac cattatttac
     6181 atactctttg attagttcaa gtctatctgg attactattg caatctataa aagtactatt
     6241 agataataag aacgtattag aacctatatc agataatatt atacagtcat actcatttaa
     6301 ctcttctaat ttatacggaa aactatctgc tgctacatgt gcaggcatat gaactgtctc
     6361 atatcctcca gattctattg cttctttcaa ccactttaca gcttcttcat aatgagttgt
     6421 aacaaacgta tcaaatcctt ttatatgagt tatatttttt acccaagatt cccctactat
     6481 taatactttt ttcatttata tattcctcct acttttataa gtttaatatt agacttgcat
     6541 acataagtat actatctaaa tactccttaa tttcaacata ttcatcaatt gaatgacatt
     6601 gttctattga acctggccct aatataagtg ttggtatttc agctaaattt ttcattaatc
     6661 tcgcatcatt gccagctgtt gaacctttta tctctaattt ttttcctaga gtttcttcta
     6721 gtatttcatg tgcattcact acaaaatcag aatccttgtc catctcaaaa cctaaccctg
     6781 attgatatat gtttatattt ggtttattat ctttaagcca taaatctcca ttagcccttg
     6841 tcattatgac atttgttaca tcatttacaa cttgttcata actcattata tttggcaaga
     6901 agtgtacaca taaattaaat acacacttat ctggcacagt agaacctgct gttccaccat
     6961 ttattacacc taaatttatt gtaggtgaag gtaatagtgg atgtttatat atcataagcc
     7021 agttatgttc caactctttt atatcttgaa gcaacaacat agccttctca attgcattta
     7081 caccctccca cttactacca cagtgaagag acacaccttt aacctcaact tcaaagaata
     7141 caaatcccat atgtgcaact attaagtttt gttctgatgg ttcacaaatt atgcaacaat
     7201 ctccaccaat accattcata actgcattta tggtaccatt tccgccacct tcttcatcta
     7261 ctactgacat tatttttaca ttaccaggta catttagacc actatctttt ataagcttta
     7321 cagctaatat tgatgctata agtccagatt tcatatcagc agttccaagt ccataaagtt
     7381 ttccattatc ttctgtagca cgatatggat tatatttcca cttacttata tcacccggtg
     7441 gcatagtgtc tacatgacca ttaaatacaa ttgtcttacc aggtaaatca tcactatact
     7501 tacaaattaa attatatcta tcttgataat tgtgacctag atttccttct ttatatatat
     7561 tttttgcttt tttaattaat tcttcgctca tttcttgtct atctacacta aagcctatgt
     7621 aatttgcaag tttttcaata tattcctgtc cttctttttc aaatccacct agtattccat
     7681 gaccaacatc ttcagtcttt atacttacaa gttcttttaa ataatctatg tattcttgtt
     7741 ttttactatt taatgtatct aagagaattt ttttcatatt tcctcctaag atttattttt
     7801 acatttaaaa tttcaagtct aatctcatct atttaagcca tagtaaactt tttcacttag
     7861 catattctca ttcaatgtat aaagatgtat gcaaattaaa gtataaaaat atatacaaaa
     7921 acttaataat ttttatagtt tataagctaa aatttaatta ctatttagat ttagctgatt
     7981 tttttaattt tatattattt atccatatag caaataacat taccaaacct tttataatca
     8041 tttgattata tgaagataca cttaataagt ttaatccatt tcctaatatt cctattattc
     8101 cgacacctat acatgtacct actatagaac cagaaccacc catcatatta gtaccaccaa
     8161 gcacaacggc tgctatagca tccaattctg ctccatctcc aacagttggt gaacctgatg
     8221 ccaaacgtga tgatagtatt atcccagcta atgctgttag tagtccagct agtgcgtata
     8281 cttttactat aactttattt acttttactc cacataatct agcggcctct tcatttccac
     8341 ctactgcatg tacatgacgt ccaaaattag ttctttttaa tagaaaatgt cctgctatta
     8401 caactataat cataattata actggtattg gtattggtcc aatatatcct ctaccgagca
     8461 tcttaaatgc atctgtattt actgatatag gtcttcctcc tgtaaataca aagcttgccc
     8521 cacttattat cgttaacatg gctaaagtta caatgaatgg aggtagtttt tgtgttgaaa
     8581 taattgcacc ttttatgact cctaatattg tccctattgc taatgcaagt atcataccta
     8641 cccatatagg tgcattataa ttcactactg ttgtagccca aaatgtacct actaaagcaa
     8701 ttactgctcc tacacttaaa tctattcctc ctgtaattat tacaaatgtc atacctactg
     8761 caagtattgc atttattgat atttgtcttc caacattaat taaattatct aaagttaaaa
     8821 agttagatga agctacactc attattataa aaattattac aagtcctaca gctgcactcc
     8881 cttggtctct aaatacactt gttaattttg tcaataatgg agaagatttt tcttctttat
     8941 gcttagttat tgaactcatt aattttacct ccgaaagcca attttaatac attattttca
     9001 accatttctt caccttctaa aaatccactt atatgacctt ctctcataac cattattctg
     9061 tcactcattg atattaattc tggtatttca gaagatatca ttattatact tacaccactc
     9121 tccactaaat ctcccattag attgtatatc tctgccttag catttacatc tatacctctg
     9181 gttggttcat ccataatcaa tatatctggt tgtaataaaa gccatcttgc tattatagct
     9241 ttttgttggt ttccaccact taaactttgt ataacttgat tcatatgtgg tgtttttatc
     9301 tttaaagctt ctatgtattt ttctaaaatc tctttttcct tttttctatc tataattgat
     9361 ttaaacttag atatcttttt taacccagcc atagttgtat tttctttgac actcatttgt
     9421 aaaaacaatc cagtaccttt tctatcttct gttattaatc ctagtccatt ttctattgca
     9481 tctgatggag ttttaaactt tacttcttca ttgtttaaat atatttttcc agaatcatat
     9541 gttctaagtc caaatatagc ttctactatt tcacttctct tacttccaac tagtccacct
     9601 attcccaata tttcaccttt tcttatatta aagctaatat cattaaaaac attgttctta
     9661 gttagatttt ccactcgcat aatttcttct tttatttcta cttttctttt tgggaaaaat
     9721 tggtctagct ttctatctat cattaaatta actaattctt cctcagacga tacatctttg
     9781 gtatttaaag tcgaaatcgt tttcccatct cgcattatag ttattctgtc acaaatctca
     9841 aagagttctt ccatcctatg ggatatatat attatactaa ctccactctc ttttaaatca
     9901 tttataagtc taaaaagtat ttcagtctct tttttactta ttgaagaagt aggttcatcc
     9961 attattaata ctttagtttc tgatgataaa actcttgcta tttctaccat ttgtttttga
    10021 gctatactaa gctctttgac taaactattt ggatttatat ctattcccat attatcgaaa
    10081 atctttttag cctcttcatt catttttttg tcattggtta agactttgcc atttatttct
    10141 tttcccataa aaatattttg tgctactgtt aattcgtcaa aaactgttag ttcttgatat
    10201 atggcattta tacctatctt tttagagata tctggggata tattctctat ttctttgcct
    10261 tcaaaaataa attttccaga agttttcgaa tgtgcaccag ttaaaatctt tatcattgtt
    10321 gatttaccag caccattttc acccagtagt gcatgaactt cgccatgaaa taactcaaaa
    10381 ttaacattat ctaatgctct gacaccagga aattctttag caatattact taacttcaga
    10441 attatattac tcataatgac actccttttt aaataggatt tctttcaaat aatctcattt
    10501 tattttacta attcttaaag tcttttatat tttccttatc ataaagtaaa gctggtactg
    10561 gtatattctt ttctaccttt tcccctttta tagcttttac tactgcttct acagctaaac
    10621 gtcccatctc tttaggttgt tgttgaatca tagcaagaat agttccatct gttattccac
    10681 ttgatgcttc ttcagttaag tcaaatccta caatcttaac atccttattt tttgcagatt
    10741 caagtgcagc tttagctcct agtacactat tttcagctgt tgcatatatt atatctacat
    10801 ctggattaga ttgaattaag ttttctactg tattaagaga ttcttctctg tcatatcctg
    10861 gttgtgaatt taatatctta acatttgctg aaccttttaa tgcatcttta aatccatcta
    10921 ttctctgcat ttgtatttga gattttaaat ctgtcactat tgctatttct gactttccac
    10981 ctaacttttc atctatgtat tttttagtgt attcccctaa ttttttacca gcatcataac
    11041 taactgttcc tacataactt gtaatatttt cagtatttaa tacagcatct actgtaacta
    11101 ctggcatttt ttcaaatgct ccttctactg ctggtactat agccgaatta tcagttccaa
    11161 gtactatcat tccatctaca ttttgttgta taaaatcttc tattgctgat gtttgcttag
    11221 ctacatcttg gtctgggtca tttactataa ctttcacacc taattcttta gctttttctt
    11281 ttattccctc atcaatatct ataaaaaatt gatgttgaag agttattgta gatactccaa
    11341 tagttaaatc tttcttgtct gaaccacctt tattatctga tttacttggt ccttccattg
    11401 aacaccctgt taacattcct attgctagta ttccagcaat tcctaaagat aaaatttttc
    11461 tcactctcat aattaacctc catttttaat agttttaaat ttgaacaaat tagattttta
    11521 tttattattt aaatactttt caacatcttc tttatagggc ataccagatt gtgcacctag
    11581 tttagttaca gaaagacctg atactactac tgcatattct actgctgtat ataaattttt
    11641 cccttctgtt atagccactg ccaatgctcc attaaatgta tctcctgctc ctgtggtatc
    11701 ttgaacatca acacttatac aagatacatg cttgatttcc tcaccatcat aaaatctagc
    11761 tccatttttt ccttctgtta ttacaagttt atttggatat ttttttaata cttcttctat
    11821 cccttcattt gtgtcaaaaa ctatcttata ttcatgttca tttggtgtta agtaagttac
    11881 tttttctatt atatcttcat ttagttttac tgctggtgct ggatttaata aaaccctaat
    11941 cctattttca aaacagaaat tcactacata atttattgtt tttaatggaa tctctaattg
    12001 tagcaatact atatctgcat tctttatctc ttcttcattc tttttaatta attctatatc
    12061 tactagctca ttagtccctg gtacaacaac aatactatta tcattttcac ataatgttat
    12121 aacagcaaca ccagaacttt tgtgattatt tctggatact aagctataat caactttatt
    12181 cgttctaaaa tttcttatta actcctctcc taagccatct tcccctatgc atgatatcat
    12241 ttttacattt gctcctagtt tagaagctga gatagcttga ttagcccctt ttcctccagg
    12301 tgatgtaaaa aaactattac ctaatactgt ttctcctttt tctggtcttt tatctacaga
    12361 acataccatg tccatgttta cacttcctat gacaactatg tttcccatta cattactcct
    12421 ttacatatct atttatgtct ctcgtgctgt tattcacttt cttttgttga atctcttatg
    12481 accaattctg gtttgaaaat caattcatca aacgattctt ttttatctcc tcttaaacaa
    12541 tttatcaata tttcactggc tttatatcct atatcataag acggctgttt tactgtggta
    12601 agagaagggc ttattagact tgatgtttga atatcatcaa atcccacgac acttatatca
    12661 tttggtatac ttaatcctct ttctttaaga cctcttatga cacctattgc aatcaaatca
    12721 tttgcacaaa atattgcatc aaaatctatc ttttctaaat tttttataaa ttcttgtccc
    12781 cattcaatct tatattttcc ttcaaataca taagcaggat tgtagcttaa attgcactcg
    12841 attagagcct ttttatatcc ttctaatcta tctactgcta tttcattttg aagtggacca
    12901 cttaggtata ttattttctt atttccaata tctaataaat gttttgtagc tatataggcc
    12961 ccttcaaagt tatttatttt aacaattcct tttaaggttt ctttgttcat gcttatcttc
    13021 ttatcaatca acacagttgg tatttttata tctttataat ttgcttcttt ccagtttgta
    13081 tttgaagatg ctgcaaatat aattccatct atcatcctct ctgatagtgt tcttattcct
    13141 tcatattctt ttttagcatc ttcatctgta ttacataaaa ttatattgta gccttcttca
    13201 ttagctttgt cttctacacc tcttgataca tctgtaaaga atggatttcg tatatctggt
    13261 ataagaagcc ctatagtatt agttttttta gttactaaac tttgtgctaa tttatttggt
    13321 acataatttt tttctttcat tattttcaaa attttttctc ttgttgcctg acttatattt
    13381 tcatctttat tattaatgac ttttgacaca gttgttttag aaacaccagc caattcgcca
    13441 atttctttta tagttatact caagaagttc acccccaaaa tttaggaaac cgttttactt
    13501 aaccggttac ctagatacta tcacatcttt tttccctttg caaccatttt atataaattt
    13561 tgatattttt cccttttttc aaaatattgc aacttataat tcaattttaa tatttaaatt
    13621 aatctgcaaa aaaaaattaa tatactttta taattatcta aaagtatatt aattaattta
    13681 agtagtattt attaaagttc aaatccttgc tctataatca tttttttatc atcttcaatc
    13741 ccacttccaa cagttgtcaa ataatcacca acaattgcag aatttactcc cccattaaat
    13801 ccaactttgt catatccctt taatgatagc cttccaccag cataccttat gtacacatta
    13861 ggaattataa acctaaatac tgctgttgtt tttagtattt ccattggttc taatacatca
    13921 taattttcca ttggtgtatt cttaatagga tttaaaatgt ttattggaaa tgattttaca
    13981 tttaattctc gtatttcaaa agccattttt agtctctgtt cttcactctc attcattcca
    14041 attataccgc ctacacatac atctagccca gcttttttag catcttttat agtttttatc
    14101 ctatctttat atgtgtgtgt tgtacatata ttatgaaaat tatctctaca cgtctcaaca
    14161 ttgtggtgat atgtagtcac acctgctgat ttcaacattt tagctttctc ataatctata
    14221 atgcctaaag atgcacataa ctttaaatta gtatttttcc tcaatccttc atagatattt
    14281 aaaatatcat taaactcctt tcctgacata cctttaccac ttgtaaccaa tgagaatcta
    14341 tgcactcctt tactctccat ctctttagct ctatttaaga tttcattgta atctaataat
    14401 gaatattctt ctacaccagt tttataatga gatgactgag cacaaaattt acagtcttca
    14461 ctacatttac cagatttagc attcattatc gtacataaat ctgcttttct tcccataaaa
    14521 tattctctta tttcatttgc agattttaat agagtatcaa aatcgttttt attatttgta
    14581 tcaagactta ttaaattaag agcttcttca taacttattt cttttcctct caatacttta
    14641 ttttttaatt ttattatgta tttttccata taccctcctc tttgtcaact taatattttt
    14701 atgcagttta cttattatat atagtattgc atatataaca tttattggtc aactttattt
    14761 taggcataag tttactattc gcaaaaaaat actgcttcaa aataatatta gtatatagat
    14821 tatacatatt attattaaag cagtattttt tattagaata taattcttac tggtttataa
    14881 aactatcctt gtcttaactc catccaagct ttccattgta ataacctcta agccttcata
    14941 ttctctaaat agccatttta aatccttcat ggcttgatta acctgacttt ttgttagatt
    15001 atttataaaa tattcttttc tagtttcctc atactctttg ctagtcaaaa agtttcttag
    15061 aaaattaaac gcaaatacac ttggaatatt gtttacattg gccttcttgc tgttttcctt
    15121 ctcatagtaa attttcataa atgcatactc ctttgatact tttagatttc tcttacaata
    15181 attatatgca tttaagtgct actattctta taattttatt aattcatatt ctttacttcc
    15241 catacccatt tcacatgcat agtctattat tctagttcca tttacatgag gatgttcatg
    15301 tttgaaaaca tcatgaccca tttccttaca aatcaaatca tagcatgctt gttcaatagc
    15361 tacagggtct gttgatgcta atattcctat atcatgggca attggtctac ctgaccaagg
    15421 tacacaatca caaagtggag ttacattcat aacaaaagtt atatacccta ccttgtcttt
    15481 tttatttgat actgctccat aagcgtattc tgccatcttt tcgacaaaca catctgagtc
    15541 actttcccat tgtatagtta ctgctctagt tggacagaca gttggacatt ctccacatcc
    15601 ataacataca tcagaatcta taactgcttt tttatccact atacttatag ctttggtagg
    15661 acaagaatta acacactttc cacaacctac acacttcttt tcctttacta ctggagtaac
    15721 atcagaatgt tgcatttgtt ttcctgctgc acttgcacat cccatagcta agttttttaa
    15781 tgctcctcca aaacctgctg cttcatgacc tttaaaatga ctcattacta tcattgcaga
    15841 agaattataa atttctccac ctatttttac actttcaaaa tgttttttgt ctatttttac
    15901 attctcataa tttctactat ataatccatc tgcaattatt actggtgcat ttacaactgc
    15961 ataagcaaat ccattttcta tagcagttgt aagatggtct acagaatttg ttctactccc
    16021 tgtatacagt gtatttgtat ctgttaaaaa tggctttcct tcgcagtctt taaccttatc
    16081 aaccacttgt ctaactgcca caggactcat atatgtagta tttccctttt ctccaaagtg
    16141 aagtttaatt gcaacttggt cattcttttc tattaaatct ttgaatcctg cttcatcaaa
    16201 taatcttctt acattgtttg gtacgttttt attttgcgaa tttgagtata aatcacaaaa
    16261 atatactttt gacttttcca tatctacacc cctttaaaat atttacttat actaatttta
    16321 tcacttttaa agtaattttt agtatatttt aacataatta aatattctta tatttatttg
    16381 atacaaa
//
LOCUS       DBGJFF010000084        16124 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.085, whole genome shotgun sequence.
ACCESSION   DBGJFF010000084 DBGJFF010000000
VERSION     DBGJFF010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16124
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.085"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            120..662
                     /locus_tag="ACOIAF_002629"
     CDS             120..662
                     /locus_tag="ACOIAF_002629"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HGZ2669633.1"
                     /translation="MKICITVGHSILKGGKSTGVNGIVDEYNYNKKLAPMLAEMLISQ
                     GNTVDVIICPERHFMSEREEFFYRVPKVNSGKYDLLVELHLNKADGTQCGTEVLYYGT
                     EGLEYAKRVSNRLGELFENRGAKKRENLYILRNTNPVAIQIESFFCDNVNDCNKANES
                     GYDYIARLITEGILNKDINM"
     gene            990..2483
                     /locus_tag="ACOIAF_002630"
     CDS             990..2483
                     /locus_tag="ACOIAF_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HGZ2669634.1"
                     /translation="MKYTFEKFKLNEKILKSLKSLGYNIPSRVQREVIPKLLKGQNLV
                     VRSKTGSGKTASFAIPLCENINVDYNNIQALIVVPTRELALQVKDEISDIGRLKKVRC
                     SAIFGKQSIKDQIAELKQRVHIVVATPGRILDHINRGSIKLENVKYLVIDEADKMFNK
                     GFVEQMEKILLNLPKEKIVSLFSATIDEEIKYICEKYMLDYSVINIEENESDTNQKTR
                     QIDDKIIKANGREKYILLKELIYSENPKSVIIFCNTKEKVSKLYNKMSKEGFLIRELH
                     ADLSQERRIFVIKDFKNQKFNILVSSDVASRGIHIDDISLVINYDVPQDKENYIHRIG
                     RTGRKGNSGKAITIVTEKDEKYIENIETYIGYKINELTDIEQSRITHGKVLFEEYSKG
                     ILKKVSKRKKVDKNSYKSKDIKLNIESEVVKLYLNAGKKKKIRVLDIVGAFSNIKGIT
                     NDDIGVIEVQDLCSYVDILNYKGDLILKKYKEIPIKKKMVKVKRDIR"
     gene            2557..2790
                     /locus_tag="ACOIAF_002631"
     CDS             2557..2790
                     /locus_tag="ACOIAF_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669635.1"
                     /translation="MKAEDMKRKVSPIDLERILVTGGVIKHEDSIWLLDFWGNKDIAG
                     ILLMPPTRHVILHLNDCCKWKEYFRTKKKFYRS"
     gene            2946..3710
                     /locus_tag="ACOIAF_002632"
     CDS             2946..3710
                     /locus_tag="ACOIAF_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGZ2669636.1"
                     /translation="MAYFNYNDKQCYYNEIGDGTPLLFLHGNTASSKMFNDIIDFYKD
                     EYKVILIDFVGHGKSQMVDKFSADLWFDEAMQVICFLEAMNYKKVNIIGSSGGALVAL
                     NVALERPDLVNKVIADSFEGEVPLESFVQNVKIEREASKQDDGAKAFYIYNQGENWEK
                     VVDNDTEAIFEHYKTIGKFFHKPLETMQPEVLLTGSREDEFVSLISSDFFENTFSLLL
                     EKIKNGKMYLFDKGGHPAILSNGLDFSNVAKKFLEE"
     gene            complement(3995..7174)
                     /locus_tag="ACOIAF_002633"
     CDS             complement(3995..7174)
                     /locus_tag="ACOIAF_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HGZ2669637.1"
                     /translation="MKIDMIYKLIRRNTEPSRWSRGVKYYTNQLVDYIDIDIDDLQNN
                     LLTFRGGVNSEDNYSFYFNSITIDIKHFKIIENDCSCADCQSKGKSIFICKHIVAIVI
                     KSLKQIKNKKDVPPILIDILGADKDKLSNTNSKTSLDLSKQLLSLLDNTKRERVNLDV
                     LINVDSNKAIAEFKIGIDKMYILKNITEFAYSKLNGYELDFGKKFTYNPNTHYFADED
                     EKLVDIIEEYGTNLSYNFSSSHSKKFMEINASSLKQFLNAIKYKDFTLIFSKEEYHPK
                     ILNDTLPVRFDFKNIEDKIVLYSKDSLPVPLTKKNDVLLYDGNIYLLSHKKAHDYSKI
                     YQILSKTKELKFDKEDSKDVLGLLVPRLKSISQEVHLDDNIKNNITKDFKSEFYFDMI
                     DGNICCDVKYIYDDEDKKFVLPNIQKEATIENKLTSSQFTKENNHYVFKGTDHDLFTF
                     LDLQLESLKEFGDIYYSEKFKLKKIYNASSIQASINQTNQNYLEFNFNISDINPKEYK
                     DILKAFQEKRTFYKLKDGNFIDLSERETKDFFELVENLDIMDKSKNNKIHKNKALYIN
                     DVIRSKNLKFIDGKKELDSICEKFRNFDSINLEIPANLNANLRDYQINGLNWFKVLDY
                     YKFGGILADEMGLGKTIQTIAFLLSLSNKKSLIVTPTSLIYNWKNEFEKFAPDIKVLL
                     IHGNKRDREKCFMELENFDVILTTYGTLRNDLEKYSEIKFDYCILDEAQNIKNPVALV
                     TESVKSINAENKFALTGTPMENNLLELWSIFDFIMPGYLYSKAKFQELFINKEDNVKN
                     LKKLIKPFILRRSKKQVMKELPDKIEKNFFVELNKEQKKIYSVYSKDIQDKMKDKNLK
                     KDKIVIFSYLTKLRQLCLDPSIVVKDYNKKSSKIETCLEILRDSINENHKILLFSQFT
                     SVLKNISKELDKYKIKYHYIDGKTNAKERLELVDEFNNSMDKKVFLISLKAGGTGLNL
                     TSADMVIHFDPWWNPSVENQASDRAHRFGQKNSVQVIKLIAKGTIEEKIIKLQESKKE
                     LINQFINGELSNEGVLKSLSDEEIINLFN"
     gene            7455..9632
                     /locus_tag="ACOIAF_002634"
     CDS             7455..9632
                     /locus_tag="ACOIAF_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HGZ2669638.1"
                     /translation="MYNKYNNLYLLLGKENVNMDSYKQLKAKIPCKETGIEIKRTLCD
                     ICSPGVHCGIDAYVKDGKVIKIEGTKEHPMNKGLLCTKGLCNREYIYREDRIKTPLKR
                     IGKRGEGKFKPISWDEAYREIAEKLNRVKSEYGAKHVAFFTGYGKWYRPFLQRFASSF
                     GSPNYGTESSSCSTSAQMAGNVATGRNFTMQDMENAKTYLGWGFNPYYSKFLNSVNLI
                     KLKEKGLKFIIVDPRITPTTQKLADLHLRIKPGTDGALALGMANLFIQKDLIDREYIE
                     KHVHGFNEYKEYVKLFDLDTVSRITGVSKDNIVKAVEMLSQGPFCINESAAPIVHHQN
                     GFQNYRAMMSLSAITGNYDRIGGNIPKQDYFAGKIGGFQTLEKEFVKEVLPKNINERI
                     GGERFPLWNEMVTQFQAMDLPRQIEEKTPYEIRALFCHGINARMFPNSGRLFKALNSV
                     DFFVNIDLFMTDTSKYADILLPACTSFEREECKSYPGGYITYTKKVIDKLYDSKSDVE
                     ILSDLANAMNIDDDLLKAGYEASVRHMFKNTCVDVDKLKESDLPLKCKDFKPYIVGSY
                     TREGYDTSTSRFELKSTIIEKYKDFGLDALPTYRGFNDNIDDEYTHLLTSGARISNAL
                     HSRLHDIKLSRILKNEASCDISSLDAKELGVISGDYIKITTKIGSLDVKVNVTDRVLY
                     KNIHMYHGYREADINSIICDNFDPYSGFPAYRSCKCKIEKMNR"
     gene            9646..10068
                     /locus_tag="ACOIAF_002635"
     CDS             9646..10068
                     /locus_tag="ACOIAF_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HGZ2669639.1"
                     /translation="MKSLFKFNKELCTACGACLLACIDQNDNNIDNGELFRHVFTIEE
                     GENIEYFSLACIHCNEPKCLGVCPKNCFKKEEGFVVLDNQNCIGCKLCEKACEYGALI
                     YGNDGKANKCNGCIERYKCGMDSPCVMVCPTGALKFNK"
     gene            10476..11279
                     /locus_tag="ACOIAF_002636"
     CDS             10476..11279
                     /locus_tag="ACOIAF_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HGZ2669640.1"
                     /translation="MKKLKLIIMFALVSALLVTGCSSGNNKNKEEQKVIRVATSGTYY
                     PFAFKDKDKLKGFEVDFWDEFAKRTNCKVEWVLTDFSGLFGLLEADKADVVSAQLTAT
                     PEREKKYAFSDAYNYSGTNIIVREDDTSIKSIDDLVGKKVGVGTGAVANEILKEKYPN
                     DEIKIVNYSSATLKGNYQDLEMGRLDAVVAQDVEALIAIKEQKLNLKMIEPPIQFGAC
                     SFALQKNEKGEKWTKEINEVIEEMYKDGTLTKISENWLGKDITKEPINE"
     gene            11272..11940
                     /locus_tag="ACOIAF_002637"
     CDS             11272..11940
                     /locus_tag="ACOIAF_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HGZ2669641.1"
                     /translation="MSNFNFEFCLSIFPFLIKALGITILISIVAFFLSILLGVILAFF
                     TQTKSKVLQYITRVYVSFFRGTPLLIQIFFFYFGFPMIFSFMKDCSPYLAIVICLGVN
                     SSAYMAEIIRASIESVDKGQQEACYAFGLSKYQSMVKIILPQAATSAVPPIVSCTLDI
                     IKGSSLGLTIGIQDLMGVAQVEAASSFKYFEIYMITILIYWAVSIILSNIQSKVEKRI
                     RRAY"
     gene            12176..12838
                     /locus_tag="ACOIAF_002638"
     CDS             12176..12838
                     /locus_tag="ACOIAF_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HGZ2669642.1"
                     /translation="MAFNMKYFLELFPTIMKSFNVTVILAISSLVFSLIIGTIVALSS
                     YYKVKVLNQICKVYVSIFRGTPLMPQLFFLYFGLAYMSDFVKNMDPVFATSIVLSLNM
                     GAYMSETIRASILSIDTGQIEAAYAIGLTNLQTMKRIVIPQAVRVALPSLFNNFIDLI
                     KGSSVAFVVGVNDIMGAAKSQGALSFKFFEVYGAVMVIYWCIITPLGWGQKKLEKKIS
                     NY"
     gene            12898..13695
                     /locus_tag="ACOIAF_002639"
     CDS             12898..13695
                     /locus_tag="ACOIAF_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HGZ2669643.1"
                     /translation="MIKRNIIKCLVAVLSLTFVITGCSKKEEVKNEKVITVASSNSAD
                     PYSYVDNGEHKGFEVDMWKEIGKRSGYKIKMEPTGFSSIFGMLDSGKVDVAANFFGMS
                     KERLEKYDASIPYGSDSVCIAVKDGNNEINKLEDLKGKVVAVSEGSQGQQVATSVNKD
                     NLFQEKVYGDGTSGLQDLDLGRVSAWIDAELTIVGDAKKANMKVRVLDKKLSATNIAY
                     FFKKNDDKSKTIKEDVNKAIEEMLADGTVKKISEKWFGMDVSADIQK"
     gene            13828..15003
                     /locus_tag="ACOIAF_002640"
     CDS             13828..15003
                     /locus_tag="ACOIAF_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HGZ2669644.1"
                     /translation="MIETKIKSIALKNREKIIKIRRQIHSNPELAFKEYKTSKLIKEE
                     LNKLNIEYINIAGTGVLATIKGKNNGGKTILLRADMDALPIKEENDLEFKSINDNMHA
                     CGHDAHVSWLLGTAMILNDIKEELNGNVKLLFQPGEEKGGSDIVIKENVLEGVDALAT
                     GHCWPTIESGKIGIARNCAMAATNTFEITIIGKGGHGAEPHNCIDPIAVGNAVYSSIQ
                     QIVSRKIDPVVPVVVSVCSFNSGVSKNIIPDVCTLQGTIRAISKEQVIEVSKILEYIV
                     KNVCKAHGADCKFEKSTGGDAVINDKNMIEIGRESACKILGAENVEIIDFPAMTGEDF
                     AIYMKEKPGLFMYIGVGDKEKNINYRLHSNKFNIDEKCLSIAPSLFSQLTVDYLCQL"
     gene            15112..15861
                     /locus_tag="ACOIAF_002641"
     CDS             15112..15861
                     /locus_tag="ACOIAF_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HGZ2669645.1"
                     /translation="MIKVENLHKSFKGTEVLKGINLEISQSEVVAILGSSGTGKSTLL
                     RCLNYLVEPTKGKIMIGDVKVDVEKATKKDITNLRKHTSMVFQNYNLFKNKTALENVM
                     EALIVVHKKSKKEAREISLKLIDKVGMLDRKDFYPSKLSGGQQQRIGIARAMAVNPNV
                     ILFDEPTSALDPELVGEVLSVIKSLAKEGITMLIVTHEINFAKEVADKIIFMDNGVIA
                     EEGTPDEIFNNPKNERTAKFLNIIRKEEVFS"
BASE COUNT         6270 a         1696 c         2664 g         5494 t
ORIGIN      
        1 aaaaatgata atcacggaga acttttacct tcttcaatta atatattatt attagtaata
       61 actttaaaat tatattacta ttatataaaa attggcttaa aagaaggtga aaaaagtaaa
      121 tgaaaatatg cataacagta ggtcatagta tactaaaagg tggtaaaagt acaggtgtaa
      181 atggtatagt tgatgaatac aattataata aaaaattagc tcctatgtta gctgagatgc
      241 ttataagtca aggtaataca gtagatgtaa taatttgtcc agaaaggcac tttatgtcag
      301 aaagagaaga atttttctat agagttccaa aagtaaacag tggtaaatac gatttattag
      361 tagagctaca tttaaataaa gctgatggaa cacaatgtgg tacagaagtg ctttactatg
      421 gcactgaagg attagaatat gctaaaagag tatctaatag acttggagaa ttatttgaaa
      481 atagaggagc taaaaaaaga gaaaatttat atatattgag aaatacaaat ccagttgcaa
      541 tacagattga aagttttttc tgtgataatg tgaatgattg taacaaagca aatgaatcag
      601 gttatgatta tatagcacga ttgattacag aaggtatatt gaataaagat ataaatatgt
      661 gagattatgg tgatttaggt attgattaat accttttaat attagatata aataaaaata
      721 caatgtatta gtataattta aactttcaaa agatagatta gtttgattat tgacaagaga
      781 tacctccttg aaaatattga tttatcagta tatttaagga ggtatttaaa tataaaaatt
      841 taattaattt gctactaaac tataggttta tagagtatat acttttatct tcagtctaat
      901 aaataatata aaaatagtag aaccttgtat tataataata taatgtagaa aaatatatag
      961 ataagataaa tataataagg tggtaatata tgaaatatac atttgaaaaa tttaagttaa
     1021 atgaaaagat acttaagtca ttgaagagtt taggatataa tataccttct agagtacaaa
     1081 gagaagttat tccaaaatta ttaaaaggtc aaaacttagt agttaggtca aaaacaggaa
     1141 gtggaaagac agcaagtttt gcaatacctt tatgcgagaa tataaatgta gattacaata
     1201 atattcaagc tcttatagtg gttccaacaa gagaattagc cttgcaagta aaagatgaga
     1261 tatcagatat agggagacta aagaaggtga gatgtagtgc tatatttggc aaacaatcta
     1321 taaaagacca aatagctgaa ttgaaacaaa gggttcatat tgttgtagct actccaggca
     1381 gaatacttga ccatataaat agaggttcta ttaagttgga aaatgtaaag tatttagtaa
     1441 tagatgaggc agataaaatg tttaataaag gttttgttga acaaatggaa aaaatattat
     1501 taaatttacc taaggagaaa atagtaagtc ttttttcagc tacaatagat gaagagataa
     1561 aatatatatg tgaaaaatat atgctagatt acagtgttat aaatatagaa gaaaatgaga
     1621 gcgatactaa tcaaaaaaca agacaaatag atgacaaaat aatcaaggca aatggaagag
     1681 agaagtacat ccttttaaaa gaattaatat acagcgaaaa tccaaagagt gtgataatat
     1741 tttgtaatac caaagagaaa gtatctaaat tatataacaa gatgagtaaa gagggttttt
     1801 taataagaga gttacatgca gatttatcac aagaaagaag aatttttgta attaaggatt
     1861 ttaaaaacca aaaatttaat atattagtta gtagtgatgt agcgtccaga ggaatacata
     1921 ttgatgatat ttctttagta ataaattatg atgtgccaca agataaagaa aactatatac
     1981 atagaatagg tagaactggg agaaaaggaa atagtggaaa ggcaattact atagtaactg
     2041 aaaaggatga aaaatatata gagaatatag aaacgtatat aggatataaa ataaatgaat
     2101 taacagatat agaacaaagt agaattacac atggcaaagt tttgtttgaa gaatattcaa
     2161 aaggaatcct aaaaaaagta tcaaagagaa aaaaagttga taaaaattct tacaaaagta
     2221 aagatataaa actaaatata gaaagtgaag ttgttaaatt atatttaaat gctggtaaga
     2281 agaagaaaat aagagtttta gatatagttg gtgcatttag taatataaaa ggtataacta
     2341 atgatgatat aggtgtaatt gaagttcaag atttatgttc atatgtagat atattaaatt
     2401 ataagggaga tttgatatta aaaaaatata aggagatacc tataaagaaa aaaatggtta
     2461 aagtgaaaag agatatacga taatttagtc aatattgatg aattgctttg ttatcgaaat
     2521 gaagttgcaa aaaacacaat agaagttgtt aaggatttga aagctgaaga tatgaaacga
     2581 aaggtctctc caattgactt agaaaggatt ttggtgactg gaggagtaat aaagcatgaa
     2641 gattctattt ggttgttgga cttttggggg aataaagata ttgctggaat tttgcttatg
     2701 cctcctactc gtcatgttat acttcatttg aatgattgtt gtaaatggaa ggaatatttt
     2761 cgtacaaaaa agaaatttta cagaagttaa attataataa attagacaaa caatagataa
     2821 attagcaagg aatgttgatt ttatctacat tctaagttat actattttta agtggctaag
     2881 ggaatagtca ttttaaatat aagtaataaa tatataccta tagggatatt aaagggggga
     2941 aatatatggc atattttaac tataacgaca aacagtgcta ctataatgaa attggggatg
     3001 gtacaccatt acttttttta catggaaata cagcctcttc caagatgttt aatgatataa
     3061 tcgattttta taaagatgaa tataaagtaa tattaattga ttttgtaggt catggaaaat
     3121 ctcaaatggt cgataaattt tctgctgact tatggtttga tgaggctatg caagtgatat
     3181 gttttttaga agcaatgaac tataaaaaag taaacataat tggtagtagt ggaggagctc
     3241 ttgttgcatt aaatgttgca cttgagagac ctgatttagt gaataaggta attgcagata
     3301 gttttgaagg agaagttcct ctagagtcat ttgtacaaaa tgtgaaaata gagcgtgaag
     3361 catcaaagca agatgatggt gcaaaagctt tttatattta taatcaagga gaaaactggg
     3421 aaaaagttgt agataatgat acagaagcta tttttgaaca ttataaaact ataggaaaat
     3481 tttttcataa gcctctagaa actatgcaac cagaggtgct tttaacagga agtagagaag
     3541 atgagtttgt atctctaata tctagtgatt tttttgaaaa tactttttca ttacttcttg
     3601 aaaagattaa aaatggtaaa atgtatttgt ttgataaagg tggtcatcct gcaattttat
     3661 cgaatggatt agatttttca aatgtagcaa agaaattttt agaggaataa tttaaccaaa
     3721 atttacaata atgaaaactg taaaatggat tgcaaaactt acaaaagtga gtgtacatat
     3781 acttcattat tatgatgaaa taggattgtt aaaacaaaat attctaatag tcaagttatt
     3841 acatttgtat atgcaatatg tatagaaaga gatatcatta gattgtaaag ttgggattaa
     3901 ataacaatat taaattttat ttattcagat attttagctt aactatataa atgttagaac
     3961 aaatttatat agttaagctg taggattaaa taatttagtt aaataagttt attatctcct
     4021 catcagatag actttttaat acaccttcat tagaaagttc tccatttata aattgattta
     4081 taagctcttt tttactttct tgtaatttta ttattttttc ttctatagtc cctttagcaa
     4141 tcaattttat aacttgtact gaattttttt gaccaaatct atgagctcta tcactagctt
     4201 gattttctac tgatggattc caccatgggt caaagtgaat taccatgtca gcagatgtaa
     4261 gatttaaacc agttccgcct gcttttaaag atattaaaaa aactttttta tccatactat
     4321 tattgaactc atctacaagt tcaagtcttt ctttggcatt agttttacca tctatataat
     4381 ggtatttaat tttatactta tcaagttcct tacttatgtt cttaagcaca gaagtaaatt
     4441 gtgaaaacaa taatattttg tggttttcgt ttatactatc cctaagtatt tctaaacatg
     4501 tttcaatttt tgaacttttt ttattgtaat cttttactac tatacttggg tctaaacaaa
     4561 gttgtcttag tttagttaaa taagaaaata taactatttt atctttcttt aaatttttat
     4621 ctttcatttt atcttgtata tcttttgaat atacactata tatctttttc tgctctttat
     4681 tcaattcaac aaaaaagttt ttttcaatct tatctggaag ttctttcata acttgttttt
     4741 tacttcttct aagaataaaa ggttttatca attttttcag gtttttaaca ttatcttctt
     4801 tatttataaa caattcctga aactttgctt tactatatag atagccaggc attataaaat
     4861 caaatataga ccataattct agtagattat tttccatagg tgtaccagtg agtgcaaatt
     4921 tattttctgc atttatactt tttacagact cagttactaa agctactgga ttttttatat
     4981 tttgagcttc atctaatata caataatcaa actttatttc actatatttt tctaaatcat
     5041 ttctaagagt accatatgtt gtaagtatta catcaaaatt ctctaattcc ataaaacatt
     5101 tttctctatc tcttttattt ccatgtatca ataaaacttt tatatctgga gcaaactttt
     5161 caaattcatt tttccaatta taaatgagtg atgtcggtgt aacaataaga ctttttttat
     5221 tagataatga taataaaaat gcaatagtct gtattgtttt accaagaccc atttcatcag
     5281 ctaatatacc accaaattta taataatcta aaactttaaa ccaattgagg ccatttattt
     5341 gatagtcacg aaggtttgca tttaaatttg caggaatttc taaatttatg ctatcaaaat
     5401 ttctaaattt ttcacatata ctgtctagtt cttttttacc atctataaac tttaaatttt
     5461 tactacgtat tacatcattt atatatagcg ctttattttt atgaatttta ttattttttg
     5521 atttatccat tatatctaga ttttcaacta attcaaaaaa atcttttgtt tctctttctg
     5581 ataaatctat aaagtttcca tctttaagtt tataaaaagt acgtttttct tgaaaagctt
     5641 tcaaaatatc cttgtactct tttggattta tatcagatat attaaaatta aattctaggt
     5701 aattctggtt agtctgattg attgaagctt gaattgatga agcgttatat atttttttca
     5761 atttaaactt ctcagaataa tatatatctc caaattcctt tagagattct aattgtaaat
     5821 ctaaaaatgt aaataggtca tggtcagttc ctttaaacac ataatgatta ttttcctttg
     5881 taaattgtga agaagtaagt ttattttcaa tagtagcttc cttttgtatg ttggggagca
     5941 caaatttttt atcttcatca tcatatatat actttacatc acaacatatg ttgccatcaa
     6001 tcatatcaaa gtagaactca gatttaaaat cttttgttat attatttttt atgttatcat
     6061 ctaaatgaac ttcttgagat atagatttta atctaggaac tagcaatcct aaaacatcct
     6121 tagaatcctc tttatcaaat tttaactctt tagtctttga taatatttga tatatttttg
     6181 aataatcatg agctttttta tgtgataata aatagatatt gccatcatat aaaagtacat
     6241 catttttttt agtaagtgga acaggtagtg aatctttaga atataagact attttatctt
     6301 ctatattttt aaaatcaaac cttacaggta gagtatcatt taatatcttt ggatgatatt
     6361 cctctttaga aaaaataagg gtaaaatcct tgtacttaat agcatttaaa aattgtttta
     6421 aagagcttgc atttatctcc ataaattttt ttgaatgtga tgaagaaaag ttgtatgaaa
     6481 gatttgtccc atattcttct ataatatcta ctaacttctc atcttcatca gcaaaataat
     6541 gagtgttggg gttgtaagta aatttcttcc caaaatctaa ttcatatcca ttaagttttg
     6601 aatatgcaaa ttcagtaatg ttttttaaaa tgtacatttt atcaattcct attttaaatt
     6661 cagcaattgc tttatttgaa tccacattta tcaatacatc taaattaacc ctttctcttt
     6721 tagtattgtc taacaaagaa agtaattgct tgcttaaatc taagctggtt ttagaatttg
     6781 tattagaaag cttatcctta tctgcaccta atatatctat tagaatagga ggaacatctt
     6841 ttttattttt tatttgtttt aagcttttta tcacaatagc aactatatgc ttacatataa
     6901 aaatactttt gccctttgat tgacagtcag cgcaactaca atcattttca attatcttaa
     6961 agtgctttat atctatagtt attgaattaa aataaaagct ataattatct tcagaattga
     7021 ctccaccacg aaatgtcaac agattatttt gtaggtcatc aatatctatg tctatataat
     7081 caactaattg attagtataa tattttacac ctcttgacca tcttgatggt tctgtatttc
     7141 ttcttattag tttatatatc atatctattt tcatattatt caccttacct cactgtatat
     7201 attataatct aaatgcattc aatttacact ctaaatttaa gactaaaatt aaggcagata
     7261 tgaatagctt ttatctatca cgatatatgt gtaaataaca atttactatt agatttttat
     7321 tgaattatct tataaactat gaatgaaaaa taattatata aatagattat taataaatat
     7381 aaaatcttat tattaaaaat attcttagaa attacactgt ataaaatata aagtatgata
     7441 tttgaataaa aagaatgtat aataagtata ataatttgta tttattattg ggaaaggaaa
     7501 atgttaatat ggatagttat aaacaactta aagctaaaat accctgtaag gaaacaggta
     7561 tagaaataaa gaggacttta tgtgatattt gttcgccagg agtacattgt ggaattgatg
     7621 catatgttaa agatggtaaa gtaataaaaa tagaaggtac aaaagaacat ccaatgaata
     7681 aagggttgtt atgtaccaaa ggtctttgca atagagaata tatctataga gaagatagaa
     7741 taaaaacacc tctaaaaaga attggaaaaa gaggtgaagg aaaatttaaa ccaatatcat
     7801 gggatgaagc ttatagagaa atagcagaaa aattaaatag agtaaaatct gagtatggag
     7861 caaagcatgt agcttttttt acgggttatg gaaagtggta tagacctttt ttacaaaggt
     7921 ttgcatcttc atttggttca cctaattatg gtacagaatc tagttcatgt tcaacatcag
     7981 cacagatggc tggaaatgtt gctactggaa gaaactttac aatgcaagat atggagaatg
     8041 caaaaactta tttagggtgg ggatttaatc catactactc taaattttta aatagtgtga
     8101 atctaataaa attaaaagaa aaaggtctaa aatttataat tgtagaccct agaataacac
     8161 cgacaactca aaaactagca gatttacatt taagaattaa acctggaact gatggagctt
     8221 tagctcttgg aatggcgaat ctatttatac aaaaggattt aatagataga gaatacatag
     8281 aaaagcatgt acatggattt aatgaatata aagaatatgt aaaattattt gatttagata
     8341 ctgtaagtag aattacagga gtttctaaag ataatatagt aaaagctgtt gagatgttgt
     8401 cacaaggtcc attttgtata aatgaaagtg cagctccaat agttcaccat caaaatggat
     8461 ttcaaaatta tagagcaatg atgtctttat ccgctataac aggtaattat gatagaatag
     8521 gtggaaacat accaaaacaa gattactttg ctggtaagat aggtggattc caaacactag
     8581 aaaaagagtt tgtaaaagaa gttctaccta aaaatataaa tgaaagaatt ggtggagaaa
     8641 gatttcctct atggaatgaa atggtgactc aatttcaagc tatggattta cctaggcaaa
     8701 tagaagaaaa gacaccatat gaaataagag ctttgttttg tcatgggata aatgctagaa
     8761 tgtttcctaa ttcagggaga ctttttaaag cgttgaatag tgtagatttt tttgtgaata
     8821 tagatttatt tatgacagat acatcaaaat atgcagatat acttttacct gcatgtacat
     8881 catttgagag agaagaatgt aagagttatc caggagggta tattacatat accaaaaaag
     8941 ttattgataa attatacgat tcaaaatcag atgtagaaat actttcagat ttagcaaatg
     9001 ctatgaatat agatgatgat ttattaaaag caggttatga agcaagtgta agacatatgt
     9061 ttaaaaatac atgtgtagat gtagataaac ttaaagaaag tgatttacca ttaaaatgta
     9121 aagattttaa gccatatata gtaggttcat atacaagaga agggtatgat actagcactt
     9181 ctaggtttga attaaaatct acaattattg aaaaatataa agattttggg ctggatgcac
     9241 taccaacata tagaggattt aatgataata tagatgatga atatactcat ttattgacat
     9301 caggagcaag aatttcaaat gcactacatt caagacttca tgacatcaaa ctttcaagaa
     9361 tattaaaaaa tgaagctagt tgtgatattt ctagcttaga tgcaaaagaa ttgggtgtta
     9421 tcagtggaga ttatataaaa ataactacta agataggaag tttggatgta aaagtcaatg
     9481 ttactgatag agtcttatat aagaatatac atatgtatca tggatataga gaagctgata
     9541 taaatagtat tatttgtgat aattttgacc cttatagtgg attccctgcg tatagaagtt
     9601 gtaagtgtaa gattgaaaaa atgaatagat aaatggagag atataatgaa aagtttattt
     9661 aaatttaata aagaattatg tacagcatgt ggagcatgtt tgcttgcatg tatagaccaa
     9721 aatgataata atatagacaa tggtgaactt tttagacatg tatttacaat agaggaaggt
     9781 gaaaatatag aatatttttc attagcatgt atacattgta atgagccaaa atgtttaggt
     9841 gtatgtccta aaaattgttt taaaaaagaa gaaggattcg ttgtattaga caatcaaaac
     9901 tgtatagggt gtaaactttg tgaaaaagct tgtgaatatg gagccttaat ttatggaaat
     9961 gatggtaagg caaataagtg taatggttgt attgagagat ataagtgtgg tatggattca
    10021 ccttgtgtaa tggtatgtcc tacaggagca ttaaaattta ataaataaat taaatataga
    10081 gaatgactta tacttaggta taagatattt ttacaaaaag ctgtattgag tttgttataa
    10141 tgaaaactca atatagcttt tttatggata tagttggata ctataatgtg attcttagta
    10201 atagtttttt gctattggac aaagactgta aaacatatta aacttatgtt gttcaaagtc
    10261 gaagtataat ataataacta gaaaattggt ttatagtgct ataaaagcaa atttagtaag
    10321 gtataaaatc agaggcaatc ctttactata atatcaatat ttgggagttc tgttattgca
    10381 gagctattaa acaattataa tggtacaagt gattcaacct ttgaaaatgt attatataaa
    10441 aaattaaaat aagattaaaa aggagagaat aaattatgaa aaaacttaaa ttaattatta
    10501 tgtttgcctt ggtatcagca ctgttagtta caggatgttc atcaggaaat aataaaaata
    10561 aagaagaaca aaaagttata agagttgcaa catcaggaac atattaccct tttgctttta
    10621 aagataaaga taaacttaaa gggtttgaag tagatttttg ggatgaattt gctaaacgaa
    10681 ctaattgtaa agttgaatgg gtattaactg attttagtgg attatttgga ttattagaag
    10741 ctgataaagc agatgttgta tctgctcaac ttactgcaac acctgaaaga gagaaaaaat
    10801 atgctttttc agatgcttat aattatagtg gaacaaacat tattgttaga gaagatgata
    10861 ctagtataaa atcaatagat gatttagttg gcaagaaagt aggtgtgggt actggagcag
    10921 tagcaaatga aatattaaaa gaaaaatacc caaatgatga aataaaaata gtaaattatt
    10981 caagtgcgac tcttaaaggg aattatcaag acctagaaat gggaagatta gatgcagtag
    11041 ttgcgcagga tgttgaggct ttaatagcta taaaagaaca aaaattaaat cttaaaatga
    11101 tagaacctcc aattcaattt ggtgcctgtt cttttgcgct tcaaaaaaat gaaaaaggtg
    11161 aaaaatggac aaaagaaatt aacgaagtaa ttgaagagat gtataaagat gggactttga
    11221 ctaaaatatc tgaaaactgg ctaggaaaag acattactaa ggagcctatt aatgagtaat
    11281 tttaattttg aattttgtct aagtatattt ccatttttga ttaaagcatt gggaataaca
    11341 atactaatat ctattgttgc atttttttta tcaatattat taggtgtaat acttgccttt
    11401 ttcacacaga caaaatcaaa agttttacaa tacataacaa gagtgtatgt ttcattcttt
    11461 agaggaacac cacttttaat tcaaatattt ttcttttatt ttggatttcc aatgatattt
    11521 tcttttatga aagattgttc accttattta gcaatagtaa tttgtttagg tgtaaattca
    11581 tctgcatata tggctgaaat tatacgagca tctattgaga gtgttgataa aggacaacag
    11641 gaagcatgtt atgcttttgg gttaagtaaa tatcagtcaa tggttaaaat aattctacct
    11701 caagcagcta catctgcagt acctcctatt gtaagctgta cactggacat aattaaaggt
    11761 tcttctttag gtttaactat aggaatacaa gatttaatgg gagtagctca agtagaggct
    11821 gcttcaagtt ttaaatattt tgagatatat atgataacga tattaattta ctgggcagta
    11881 tctataatat tgagtaatat acaaagcaaa gttgaaaaaa gaataagaag agcatattag
    11941 atttcaatat ttaaaaatgg gatgatatag atatattatt acttgtaaat aaatttttaa
    12001 ttttagaaaa ataaacttag taatctaaat tattgagaaa atatagtatc aaaatggact
    12061 gtataatcaa gttaaaaaat agtttttcta ttgaaaaaat aaataaaatt ctattgtaat
    12121 ttaatatgcg ataaagtttt ataaaaaaca acattaagct agggggactt attttatggc
    12181 ttttaatatg aaatattttt tagaattatt tccaacaata atgaaatcgt tcaatgtaac
    12241 tgtgattttg gcaataagtt cattagtatt ttcattaatt ataggtacaa tagtagcgtt
    12301 atcatcttat tacaaagtga aagtattgaa tcaaatatgt aaagtatatg tatctatttt
    12361 tagaggtaca cctcttatgc cacagttatt ctttttatat tttggactag catatatgag
    12421 tgattttgtt aaaaatatgg acccagtatt tgcaacatct atagtactta gtttaaatat
    12481 gggagcttat atgtctgaaa cgataagggc atctatactt tcaattgata caggtcagat
    12541 agaagcagca tatgcaatag gattaacaaa tttacaaaca atgaaaagga tagtgatacc
    12601 acaggccgta agagttgcac tgccatcatt attcaataat tttatagatt taattaaagg
    12661 gtcttctgta gcttttgttg ttggagtaaa tgatataatg ggtgctgcca aatcacaagg
    12721 agcgttgagt tttaagtttt ttgaagtgta tggagcagtt atggtaattt actggtgtat
    12781 aataactcct ttaggttggg ggcaaaaaaa attagaaaag aaaataagca attattaagt
    12841 tattaagaaa atacataaaa gtatttttat ataaaaaatg gtgtgaggag aagagatatg
    12901 ataaaaagaa atattataaa atgtttagtg gcagttttat cgttaacgtt tgtaattact
    12961 ggttgttcaa aaaaagagga agtaaagaat gaaaaagtta taactgtagc ttcatctaat
    13021 tcagctgacc catattctta tgttgataat ggtgagcata agggttttga agttgacatg
    13081 tggaaagaga ttggtaaaag aagtggatat aagataaaaa tggaaccaac aggttttagt
    13141 tctatatttg gtatgttaga ttctggtaaa gttgatgtgg cagccaactt ctttggaatg
    13201 tctaaagaaa gattagaaaa atacgatgct tctatacctt atggttctga ttctgtgtgt
    13261 atagctgtaa aagatggaaa taacgaaatt aataagcttg aagatttaaa gggaaaagtt
    13321 gtagcagttt cagaaggttc tcaaggtcaa caggttgcaa ctagtgttaa taaagataat
    13381 ttgtttcaag aaaaggttta cggtgatgga actagtggtc ttcaagattt agatttggga
    13441 agagttagtg catggattga tgcagagctt actattgtgg gagacgctaa aaaagctaat
    13501 atgaaagtaa gagtattaga taaaaaatta tcagctacta atatagcata cttctttaag
    13561 aaaaacgatg ataaatctaa aacgataaaa gaagatgtaa acaaagcaat agaagaaatg
    13621 cttgcagatg gaacagtgaa aaaaatatct gaaaaatggt ttggaatgga tgtaagtgct
    13681 gatatacaaa aataaatact ttaaaattaa aatgtaaaac tgtgataact atagataagt
    13741 atactaaatt tatctatagt tatctgtgta ttacaatgta tttagattag tctttataaa
    13801 tcaaaatgag ttatggaggt attgcaaatg attgaaacta aaataaaatc cattgcacta
    13861 aaaaatagag aaaaaattat aaaaataaga cgacagatac acagtaatcc agagttagca
    13921 tttaaagaat ataaaacatc taaacttata aaagaagagc taaataaact taatatagaa
    13981 tacataaata ttgctggtac aggagtatta gctactataa aaggaaaaaa taatggtgga
    14041 aaaactatat tactcagagc ggatatggat gctcttccaa tcaaagagga aaatgattta
    14101 gaatttaaat caataaatga taatatgcat gcttgtgggc atgatgcaca tgtttcatgg
    14161 ttgttaggaa cagcaatgat tttaaatgac ataaaagaag aattgaatgg aaatgtaaaa
    14221 ttactatttc aacctggcga agaaaaaggt ggttctgata ttgtaattaa agaaaatgta
    14281 cttgaaggtg tggatgcatt agcgactggt cattgttggc caactattga gtcaggaaaa
    14341 attggaatcg ctagaaattg tgctatggca gcgactaata cttttgaaat aacaataatt
    14401 ggcaagggtg ggcatggagc agagcctcat aattgtatag acccaattgc agttggaaat
    14461 gcagtatatt catccattca acagatagtt tcaagaaaaa ttgacccagt tgtacctgta
    14521 gtagtgtctg tttgttcttt taattctggt gtttctaaaa atataatacc agatgtatgt
    14581 acacttcaag ggactattag agcgataagc aaggaacaag taattgaagt aagtaaaatt
    14641 ctagaatata tagtaaaaaa tgtatgtaaa gcacatggag cagattgcaa atttgaaaag
    14701 agtacaggag gagatgctgt tataaatgac aaaaacatga tagagatagg aagagaaagt
    14761 gcttgcaaaa tacttggagc tgaaaatgtt gaaataattg attttcctgc aatgacaggt
    14821 gaggattttg ctatttatat gaaagaaaaa cctggtttat ttatgtatat aggtgtagga
    14881 gataaagaaa aaaatataaa ttacagattg catagtaaca agtttaatat agatgaaaaa
    14941 tgtcttagta tagcaccttc attattttca caacttacag tagattattt atgtcaacta
    15001 tagagtacat tcataaaaat atatttagcc tatttttaaa tttaatgcta gtatattaat
    15061 tttgatgtag aacatattat ttattatatt tatttaaaaa ggtggataac aatgataaaa
    15121 gtagaaaatt tacataaaag ttttaaaggg acagaagttc ttaaaggtat aaatttagag
    15181 ataagtcaaa gtgaagtagt agctatactt ggatcatctg gaacaggtaa atctacgtta
    15241 cttagatgtt taaattattt agttgagcct acaaaaggaa agataatgat tggtgatgta
    15301 aaagtagatg tagaaaaagc aacaaaaaaa gatataacca atttaagaaa acatacatct
    15361 atggtgtttc aaaattataa cttatttaaa aataaaacag cattagaaaa tgtaatggag
    15421 gcattgatag tagttcataa aaagagtaag aaagaagcta gagaaatatc tttaaaatta
    15481 atagataaag taggtatgtt ggataggaaa gatttttatc catcaaagtt gtctggggga
    15541 cagcaacaaa ggataggtat agcgagagcg atggcagtaa atccaaatgt aatattattt
    15601 gatgagccta catctgcact tgacccagaa cttgttggag aagttttaag cgttataaaa
    15661 tctcttgcaa aagaaggtat aacgatgctt attgtaactc acgaaataaa ttttgcaaaa
    15721 gaagttgctg ataaaattat atttatggat aatggtgtaa tagctgaaga aggaactcct
    15781 gatgaaatat ttaataatcc taaaaatgaa agaacagcaa aatttttaaa tatcattagg
    15841 aaagaagaag tatttagtta aaagtgtata aattaagcat tagaagatat gtaagatacc
    15901 cccattaaat ttataagtta gataataaat ttaatgggag tatattttta ttattagatt
    15961 tattatttaa tttaaaatta gaaatgtaac tatttgaaaa ttctttttaa aagtatatca
    16021 aaatatcaaa tacataaaaa aataatattt gacaaagtat catatgtgtt tacaaaatca
    16081 actttatgga gttatatagt ataggaatta caatgaatat acta
//
LOCUS       DBGJFF010000085        16061 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.086, whole genome shotgun sequence.
ACCESSION   DBGJFF010000085 DBGJFF010000000
VERSION     DBGJFF010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16061
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.086"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            <1..805
                     /locus_tag="ACOIAF_002642"
     CDS             <1..805
                     /locus_tag="ACOIAF_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2669646.1"
                     /translation="RESDFKKLRQYQNNNYEELTSKESVYYVSDSYKKIFFKDFEIEK
                     INLHIKDTNEYDINLFKVQKSILEPEINSQATFDLIVVKDDVFNQLKLHGNSRLIRLI
                     DIKNEKKELDLTLKLKNIVSENMKFTYPFNFTSSIESYNNLIKLSGLMLFIGIFLSVV
                     FLLCTGSIILFKQLSNIYDDKERYIMLIKLGANNKDIEKIISKQLKVIFLMPLVVGTV
                     HNLFAMSIAQKFIPRSLLVPIIITLVIYFIGYFIYYFLTLKYALNMIKE"
     gene            1030..2643
                     /gene="ggt"
                     /locus_tag="ACOIAF_002643"
     CDS             1030..2643
                     /gene="ggt"
                     /locus_tag="ACOIAF_002643"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HGZ2669647.1"
                     /translation="MKFNAYQHKYSSIRNVVYAKNGAVATSTPLASQAGLEILKKGGN
                     AVDAAVATAATLAVVEPTSNGIGGDAYALVWIEEEKRLYGLNSSGFAPENMELKNYNN
                     MKEMPKYGFGSVTVPGIPAAWSELNKKYGKLSLMECLSPAINYAREGYVVSPNVAKVW
                     KKSYELYEKELIGEEFKPWFDTFSKDGKAPEAGDIFICEEQASTLEEIANTQAESFYR
                     GRLADKIDEYSKKFNGAIRKSDLECFYPTWVEPISTEYKGYKIFEIPPNGHGITVLMA
                     LNILKELELEGNIENVEDIHKIIESLKLAFADSKTYVTDIEHMKVKIQELLSQEYAKK
                     RSLLIDNKEALYPTAGEPYCGGTVYLCTADKDGNMVSYIQSNYINFGSGIVIPRTGIA
                     LHSRGNNFNLDPKHHNVVKPFKKPYHTIIPGFLGKEDKAIGPFGVMGAFMQPQGHIQV
                     LTNMIDFGLNPQEALDAPRWQWIKGKEIEVEPEMPKHIIDSLIEKGHEIKVIHDTVDM
                     GRGQIIFKTEQESYICGTESRCDGHIAVY"
     gene            complement(2776..3627)
                     /locus_tag="ACOIAF_002644"
     CDS             complement(2776..3627)
                     /locus_tag="ACOIAF_002644"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HGZ2669648.1"
                     /translation="MKNIISAKELISKLEKNDNLVVIDCRFDLINRTYGIDSYKKGHI
                     KGSFILDIDKDLSSPTKEHGGKNPLQDPLILKEKLEKMGVDNDTTIVTYDDGDLNGAC
                     RLFFQLKHLGLKNVYVLDGGITSFVKEGGELEEKVNIPSCTGKEIRPNINNGLVVPME
                     YVKSKLYKKDTVIIDCRANERYQGLVEPAYSKAGHIPSAKNYFCKDLIKSDFENGSLK
                     DIEFLKKFFKDLNNYDEVILSCGSGISACVNSLALRELDIPHKIYIGSFSDWISYDDN
                     EIKTGQE"
     gene            4253..5146
                     /locus_tag="ACOIAF_002645"
     CDS             4253..5146
                     /locus_tag="ACOIAF_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HGZ2669649.1"
                     /translation="MSNKIVSKRQLTDSIYLMEIEAPRVAKSSQPGQFIIIKNDEKGE
                     RIPLTIADYDREKGTVTIVFQTVGASTKKLAMFEENDFVMDFVGPLGQASEFIHEDIE
                     ELRNKKILFVAGGVGSAPVYPQVKWFKEHGLDVDVIIGARTKELIILEDDMKKVAKNV
                     YVSTDDGTYGFNGRVTDLLKDLVDNQGKKYDQAIVIGPMIMMKFMCQLTKELNIPTIV
                     SLNTIMIDGTGMCGGCRVSVGNETKFACVDGPEFDGHLVDFDQAMRRQSMYKTQEGRA
                     MLKLEEGDSHHHSNCGCGGNK"
     gene            5146..6540
                     /gene="gltA"
                     /locus_tag="ACOIAF_002646"
     CDS             5146..6540
                     /gene="gltA"
                     /locus_tag="ACOIAF_002646"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HGZ2669650.1"
                     /translation="MDAKKVKVPVREQEPAVRATNFDEVCLGYNKEEAMAEANRCLAC
                     KKPKCVGGCPVGIDIPGFITKIKEDDIEGAAKVIAKSSSLPAVCGRVCPQESQCEGVC
                     ILGIKSDAVSIGKLERFVADWSKENDINLSDTEPKKNQKVAVIGSGPAGLACAGDLAK
                     KGYDVTIFEAMHEPGGVLTYGIPEFRLPKQAVVQPEIDNIRKLGVKIETNVIVGKTIT
                     VDELIEDEGFEAIFIGSGAGLPMFMNIPGENANGVFSANEFLTRVNLMKAYRDDYDTP
                     ISSGKKVAVVGGGNVAMDAARTALRLGSESYIVYRRSEKELPARAEEVHHAKEEGIIF
                     NTLTNPKEILVDENGYVKGMVCIRMELGEPDDSGRRRPIEIEGSEFVLDVDTVIMSLG
                     TSPNPLISSTTKSLDINKKRCLITDENGQTSKEGVFAGGDAVTGAATVISAMGAGKTA
                     AASIDEYLKAKVNA"
     gene            6906..9125
                     /locus_tag="ACOIAF_002647"
     CDS             6906..9125
                     /locus_tag="ACOIAF_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HGZ2669651.1"
                     /translation="MKKREKVFDGLVFKHILILLILLVLIDLTSTIIYASNSMEITSR
                     NMIEASKRELENYLEVNISLLKALSQDDRFSNGETSLIEKGKLLSPYQKEYNLFMIGI
                     TDTKGNASSTYRENVNSIKDKPSFEKAIKTKQVVVSDIEVSNVTGDKVFIIYVPIVKN
                     NEMIGTIFASFYFQDVNNLISRSNFDDSIKFLMIDKDYTIISHPNKKYVNDKSKMLDL
                     EGNIIGTTKSEILKNINKKCQGDFLSWDNWRLYNVKYTNIKWTNWTLVSKCNIFKNFK
                     NLIVNFMIKLYFYIVIFMILWKLSNAKLIEQLKKLAYYDSLSGIKNKEKFRKDSMYIL
                     KNYYQDNFYLVQLDVNKFKYINEMFGYAEGNKILIHISQVLNNNTNKYEICARMDNDH
                     FILLIACNTEDELLNRLSKINKEICNLNTTNSSKYKIVMSSGIYKITKKDDIKKIDLL
                     IDRANIAAKSKKEKYEHSYSFFNEDTRNRLYKEKRLEDNMNKALEKGEFIVYYQPKYS
                     LDDVNEIEGAEALIRWNSPEFGFISPIDFVPLFEKNGFIVNIDMFVFEEVCKTLNKWI
                     NKGYTPVPISVNMSRVHLYRDNFIENITDLISKYNISPEFIELELTESVVFDNLNILI
                     DIMKKIKEIGFLISMDDFGSGYSSLNLLKDLSFDILKLDRGFLIETTDTKRGKIIISK
                     IVEMAKAIDIKVICEGVETYEQVEFLKEIGCDKVQGYLFAKPMVLDEFEKHLNFKFD"
     gene            9272..10018
                     /locus_tag="ACOIAF_002648"
     CDS             9272..10018
                     /locus_tag="ACOIAF_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2669652.1"
                     /translation="MNILSIRNISKTYCGNIPFKALDKVSLNIEKGEFVSVMGPSGSG
                     KSTLLNIISTVDRQSEGEVVLDGYDVSKLKGEKLAEFRRKQLGFVFQDFNLIDTLTVG
                     ENIMLPLTLEGSSIKDMNIQTKSISKFLGIDKILDRKTYEISGGQAQRCAIARAIINK
                     PAILLADEPTGNLDSKSTDDVLKLFTRINKEQKVTTLMVTHEAYSASYSDRVIFIKDG
                     CIYTEIKKSESSNSFYSDILAVLSQIGGVR"
     gene            10018..11898
                     /locus_tag="ACOIAF_002649"
     CDS             10018..11898
                     /locus_tag="ACOIAF_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2669653.1"
                     /translation="MDFRKFAYNNIVRNFRAYLAYFLSSVFSIMIFFVFAVSMFHPII
                     TKSGMQSGSTAFMALMTSELIIFGFSLLFILYSLGNFLKYRLKDFGVLMIIGMSNRQL
                     KKLILIENLIIGFLAVMLGILLGLSISKLFLLYLSKLFYMNLTEFYFPVKAMLLTIVS
                     FMILFLITGPMSLKLLSKKSILELLVGTKKPKKEIKSSKLFGIIGVICLILGYAMILF
                     GNKLKINGGMYTTALLITLGIFLFFSQFTILILKYLKNKEKFYKNKVNMLLISNLIYK
                     LKDNTRLLFLITILLSATLVAFTTTSTLVSSQGEASKNNFPMVYSYFSEHNNEKEYEE
                     LQQIREAIKGYDYKELSFPVLKYNREVLLSVKNYNKLSKELGLDKISSKLEKYEGIII
                     PSSNSQEHINSLKQLKNYSIKNLNIEVKKSKNKIIFPTGLFSKVVVISDDLYNEVKGE
                     FSKINFHGFDYKNWQSSAKITENLKEKHFNLDREYNRSYFLTLPDMYLVELQQSKILQ
                     FMGVFIGVILFIAVFSFLYFRLYTDEIEDNVKYKKLSKIGLSFKNMNKIVSIEIGTLF
                     FIPYFIAIINSIISLLFLNQVLNNSFKLENLGILLLISSIYSLYFYLLKKIYTRKVWF
                     FD"
     gene            12017..13408
                     /locus_tag="ACOIAF_002650"
     CDS             12017..13408
                     /locus_tag="ACOIAF_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HGZ2669654.1"
                     /translation="MENQQLLGTERISKLLLKYSIPAIIGMLVNSLYNVVDRIFIGNI
                     PGVGPLAITGLGVTMPIMTIILAFGMLIGIGTTTTISIKLGQGKVEEARKLIGNAMTL
                     SVITGIIIMILGILFANKILTLFGASENTLIYAKSYINIILLGTVVNLLSFSLNHSIR
                     ADGSPKISAGIMIVGCLTNIVLDWILIFGFNLGIQGAAIATVTSQALTAILTIGYYIS
                     GKSNLRFSKSNLKLDKKLIKAVFAIGMSPFAMQLAASLVQVISNIALKTHGGDLAIGA
                     MATISSIAMVFLMPIFGINQGAQPIIGFNYGAEKYDRVKKAYLGSLVVATIILCMGMV
                     VVMLFPEAIIGIFNKDPELMNISVNGLRIYLLMLPIVGLSVTGTNFIQSIGKAKMAML
                     LSLLRQVILLIPAVLILPTFLGLQGVWTAQPVSDFIATVITGIVVFRELKRYTPKTEK
                     LNENERLNEITTE"
     gene            13515..14555
                     /locus_tag="ACOIAF_002651"
     CDS             13515..14555
                     /locus_tag="ACOIAF_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HGZ2669655.1"
                     /translation="MEFTKKSKLKNMYVNMNVARMLSKINEYKGRQLLYKKQPKEILE
                     NLEKKSLVDCSESTYIGNQRDSSNFNLEKLISNEVTPRSREELSIVEYRDVVKTINSA
                     YESIPISSQTILELHGYLYKFSSTRGGSYKSDNDFIEHNLKPSEFISIDSSVNKVEKA
                     VEEICEAYNTLIEEDEIDILILISAFVLDFILIHPFKEGNIKMARVLILLLLNKNGYE
                     VGRYISLGKIFDDSSYEYYSNLNYLKASIGSEKADMNAWIEYFLETILTAYEKLDDSL
                     NISDKKRQTKTSRIEKIINSTLGYFTKEDIRDLCPDIPEPTINRVFNNLRKQDKIEVV
                     ARGRSAKWKKKY"
     gene            15044..15697
                     /locus_tag="ACOIAF_002652"
     CDS             15044..15697
                     /locus_tag="ACOIAF_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HGZ2669656.1"
                     /translation="MKKKLLYINVNSKPEDLSSSKTVARKFINKFMEKNKDFEVEEID
                     LYKEHIPRLEYQYFEKRNSIVSEENAKNLDDKDRKELTKIRNLCDQFVSASVYVIAAP
                     MWSLSFPAPLKEYIDCIIQDGKTISFEGNDKPQGILNDIDRSMVYIQSSGGHIPWVLK
                     PVMNKGLNYVESIMKFIGIKKFDELLVDGTGTSEEERQAAIEKASEKIDGIIDGMKF"
     gene            15773..15982
                     /locus_tag="ACOIAF_002653"
     CDS             15773..15982
                     /locus_tag="ACOIAF_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669657.1"
                     /translation="MREEKSNEKYDCYWCNQENNFCVEIKDNIVMIDDGTGTLKQAVF
                     IGYKQIQINLNCSHCQNLNRIKLNL"
BASE COUNT         6370 a         1639 c         2747 g         5305 t
ORIGIN      
        1 tagagaaagt gattttaaaa aattaagaca atatcagaat aacaattatg aagaattaac
       61 ttcaaaggaa tctgtgtact atgtttctga ttcatacaaa aaaatatttt ttaaagactt
      121 tgagattgaa aaaataaact tgcatattaa agatacaaat gagtatgata taaatctgtt
      181 taaagtacaa aaaagcatct tggagccaga aataaattca caagctacgt ttgatttaat
      241 tgtagtaaaa gatgacgtat ttaatcagtt aaaattacat ggaaattcta gattaataag
      301 attaattgat ataaaaaatg aaaaaaaaga actggatttg actttaaaat taaaaaatat
      361 agtaagtgaa aatatgaaat ttacttatcc ttttaatttt acatcaagta ttgaaagtta
      421 taataacttg ataaaattaa gtggtctaat gctgtttata ggcatatttt tgtctgtagt
      481 atttctttta tgtacaggta gtataatctt atttaaacaa ttatcaaata tatatgatga
      541 taaggaaaga tatattatgt taataaaact tggagcaaat aataaagata ttgagaaaat
      601 aatatcaaaa cagttaaaag ttatattttt aatgccatta gttgtaggta cagttcataa
      661 tttatttgct atgtctatag cacaaaagtt tataccaagg tcattacttg taccaattat
      721 cataactcta gtcatttatt ttataggata ttttatatat tattttttaa ctttaaaata
      781 tgctctaaac atgataaaag aataaattat ttttaagtca aaagaattag atatatgttt
      841 tgaataaaat aacttattta agtgcagata gaaaatacta gaattgagtt taagatattt
      901 atttaaagat aaaattaaag ttaggaaaaa gtctatattt tatatcattt tgtatcagtt
      961 acattacttg atgttatata ataaatctaa gcagaatatc aaataaaaaa tgacgaggag
     1021 ctgaaattta tgaagtttaa tgcctaccaa cataaatact catcaataag aaatgtcgtg
     1081 tatgctaaaa atggagcagt tgcaacatca acaccattag catctcaagc tggacttgaa
     1141 atactaaaaa agggtggaaa tgctgttgat gcagcagttg caacagccgc cacattagca
     1201 gtagtagaac ctacaagcaa tgggattgga ggagatgcat atgcccttgt ttggatagag
     1261 gaagaaaaac gattgtatgg tttaaattct agtggctttg ctccagaaaa tatggaatta
     1321 aaaaattata ataatatgaa agaaatgcca aaatatgggt ttggatcagt aactgtacct
     1381 ggaatacccg cagcttggtc agaattaaat aaaaaatatg gtaaacttag cttaatggaa
     1441 tgcttatctc cagcgataaa ctatgcaaga gagggatacg tagtatctcc taatgtagct
     1501 aaggtatgga aaaaatcata tgaactctat gaaaaagaac ttattggaga agagttcaaa
     1561 ccatggtttg atacattttc taaagatggt aaagctccag aggctggaga tatatttata
     1621 tgtgaagaac aggcaagtac attagaagag atagcaaata ctcaggctga aagcttttat
     1681 agaggaagac ttgctgataa aatagatgaa tattctaaaa aatttaatgg ggctataaga
     1741 aagagtgact tagagtgttt ttatccaaca tgggtagaac ctataagtac agaatataag
     1801 ggatataaaa tatttgaaat cccaccaaat ggtcatggaa taacagtttt aatggcactt
     1861 aacatattaa aagaattgga attagaagga aatatagaaa atgtagaaga catacataaa
     1921 attatagaga gtttaaaatt agcttttgca gattctaaaa catatgtaac tgatatagag
     1981 catatgaagg taaagattca ggaattacta agccaagagt atgccaaaaa aagaagttta
     2041 ttaattgaca ataaagaggc tttgtatcca actgcaggag agccatattg tggtggtaca
     2101 gtataccttt gtactgcaga taaggatgga aatatggttt catatataca gagcaattac
     2161 ataaattttg gttcaggaat tgtgatacca agaactggga ttgctttaca tagtagagga
     2221 aataatttta atttagaccc taaacaccac aatgtagtaa aaccatttaa aaaaccttat
     2281 catactataa tacctggatt tttaggtaaa gaagataaag caataggtcc atttggtgta
     2341 atgggagctt ttatgcaacc acaaggacat attcaggtgt taacaaatat gattgatttt
     2401 ggattaaatc cacaagaggc gctagatgct ccaagatggc agtggataaa aggaaaagaa
     2461 atagaagttg aaccagagat gccaaagcat ataatagatt cattaattga aaagggtcat
     2521 gaaataaaag taattcatga tactgttgac atgggacgag gtcaaataat atttaaaaca
     2581 gaacaggaaa gttatatttg tgggacggaa tcaagatgtg atggtcatat tgcagtttat
     2641 taatattatc aactaaaata tatataataa atttaaagag tatatgaaat aataagtaaa
     2701 ggcgaaagtc tacaatgact gacgccttta caaaatttaa gctatcatat gctgatttta
     2761 tatagattat gagccttatt cttgaccagt tttgatttca ttgtcatcat aactaatcca
     2821 gtcgctaaag ctaccaatat aaatcttgtg aggtatatcc agttctctaa gagcaagact
     2881 gttaacacaa gcagatatcc ctgaaccaca agataaaatt acttcatcat aattatttaa
     2941 atctttaaag aattttttta aaaattctat atctttaagt gagccatttt caaagtcact
     3001 ttttattaaa tccttacaaa aataattttt tgcacttggt atatgaccag cttttgagta
     3061 agcaggttca actaaacctt ggtatctctc attagcacgg caatctatta ttacagtatc
     3121 ttttttgtat aatttagact taacatattc cattggaaca actaaaccat tgtttatatt
     3181 tggtctaatt tccttacctg tacaacttgg tatattcact ttctcttcca attctccacc
     3241 ttcctttacg aaagatgtta ttcccccatc tagtacatag acatttttaa gccccaaatg
     3301 ttttaattgg aaaaataatc tgcaagctcc atttaaatct ccatcatcat aagttacaat
     3361 cgtagtgtca ttatcaacac ccattttttc taatttttct tttagtatta aagggtcttg
     3421 taaaggattt ttaccaccat gttcttttgt aggagatgat aaatccttgt ctatatctaa
     3481 gataaaactg cctttgatgt gtcctttttt ataagaatca ataccataag ttctattgat
     3541 aagatcaaat ctgcaatcta ttacaactag attatcattt ttttctagtt ttgatataag
     3601 ttcttttgct gaaataatgt ttttcaaaga atgtacctcc tgtgtttata ttatctttat
     3661 tttaatttat attaattatt aactttataa actaatatta ttataattta ttacataagt
     3721 aaataaatct ttagaaattg taaaaatttt atataaaaat tcaaataatt aatgaatatt
     3781 gaaaatttta gatatataat aaaataagag tatattttat acaatatgaa agctggtatt
     3841 gcaatagatt aaagccttat acacgtttag tttttaaaga aattagtgtt attatgaaaa
     3901 cctaagttaa tttattaaat aaataattga ataaaaaaat tttattatgt atttataaaa
     3961 tttttgttac aaatttggca taatatagta tactttaagt gtgtattagg ttaaatctat
     4021 aacaaattag acgtatgata tagacttagt gtttattggc atttacaaga aatttgttaa
     4081 tatcaactat ttgtagtttg ggcagttata attattatag gggggatgtc caatacaaaa
     4141 caatcaacca agaactaata ttatattata aagagttata gttttgtgtg tttatgtttt
     4201 taattaaatt taaagtggga aaaatatatt tgttagaatg gagaatagcg agatgagtaa
     4261 taaaatagtt agtaagagac aattaacaga tagtatttat ttaatggaaa tagaggcacc
     4321 tagagttgca aaatcttctc aaccaggtca atttataata attaaaaatg atgaaaaagg
     4381 agagaggatt cctcttacaa tagctgacta tgatagagaa aaaggaacag taactattgt
     4441 attccaaaca gttggtgctt caactaaaaa gttagcaatg tttgaagaaa acgattttgt
     4501 aatggatttt gtagggccat taggacaagc aagtgaattt atacatgaag atatagaaga
     4561 attaagaaat aaaaagatat tatttgtagc tggtggagtt ggttctgctc cagtttatcc
     4621 acaagttaaa tggtttaaag agcatggatt agatgtagat gtaataattg gtgcaagaac
     4681 taaagaatta attatattag aagatgatat gaaaaaagta gctaaaaatg tatatgtatc
     4741 aacagatgat ggtacatatg gatttaatgg aagagttaca gacctattaa aagatttagt
     4801 tgataatcaa ggtaaaaaat acgaccaagc aatagtaatt ggaccaatga taatgatgaa
     4861 atttatgtgc caattaacta aagaactaaa tatacctact atagtaagtt taaatacaat
     4921 aatgatagat ggaacaggta tgtgtggtgg ctgtagagtt agtgttggaa atgaaactaa
     4981 gtttgcatgt gtagatggtc ctgaatttga tggtcattta gttgattttg atcaagcaat
     5041 gagaagacag tctatgtaca agactcaaga gggaagagct atgctaaaac ttgaagaagg
     5101 cgatagtcat catcatagta attgtggatg tggaggtaac aaataatgga tgctaaaaaa
     5161 gtaaaagtac cagttagaga acaagaacct gctgtgagag caacaaattt tgatgaggta
     5221 tgcttagggt ataataaaga agaagctatg gcagaagcta atagatgttt ggcttgtaaa
     5281 aaaccaaaat gtgtgggtgg atgtccagta ggaatcgaca taccagggtt tataacaaaa
     5341 attaaagaag atgatataga aggtgctgct aaagtaatag ctaagagcag ttcattacca
     5401 gctgtttgtg gtagagtatg cccacaagaa agtcaatgtg aaggtgtatg tatacttgga
     5461 ataaaatcag atgctgtatc aataggtaaa ttagaaagat ttgtagcaga ttggtcaaaa
     5521 gaaaatgaca taaatttatc tgatacagag cctaagaaaa atcaaaaagt agcagtaata
     5581 ggaagtggtc cagcaggtct agcttgtgct ggtgatttag ctaaaaaagg ctatgatgta
     5641 acaatctttg aagcaatgca tgagccaggt ggagttttaa cttacggaat acctgagttt
     5701 agacttccaa aacaagctgt tgttcaacct gaaatagaca atataagaaa attaggtgta
     5761 aaaatagaaa ctaatgtaat tgttggtaaa acaattacag tagatgaact tatagaagat
     5821 gaaggatttg aagctatatt cataggttca ggagctggtc ttccaatgtt tatgaatata
     5881 ccaggggaaa atgcaaatgg agtattttct gcaaatgagt ttttaactag agtaaactta
     5941 atgaaggctt atagagatga ttatgataca cctattagtt ctggtaaaaa agttgctgta
     6001 gtaggtggtg gaaatgttgc tatggacgct gctagaactg cattaagatt aggttcagaa
     6061 tcatatatag tttatagaag aagtgaaaaa gagcttccag caagagctga agaagttcat
     6121 catgcaaaag aagaaggcat tatatttaat acattaacaa atcctaagga aattttagta
     6181 gatgaaaatg gatatgttaa aggtatggtt tgtataagaa tggaattagg agagccagat
     6241 gattctggaa gaagaagacc tatagaaata gaaggttctg aatttgtact tgatgtagac
     6301 acagttataa tgtcacttgg aacaagccca aatccactaa tatcttctac aacaaagagt
     6361 cttgatataa ataagaagag atgcttaata acagatgaaa atggtcagac ttctaaagag
     6421 ggtgtatttg caggtggaga tgctgttact ggagcagcaa cagtaatatc tgcaatgggt
     6481 gctggtaaaa cagcagcagc atcaatagat gaatatttaa aagcaaaagt taatgcttaa
     6541 tttgtaaatt aaagagaggg tgtctcaaaa tgaacttttt agttcatgag gcactctttt
     6601 tgtacaaaat caaatatgtt ttaatcattt caacaatgaa aatttcattc ctgtagaaag
     6661 tctaactttt aggtcttagt acaatctcta ttttgatgta gccacttttt tagatggttt
     6721 aataatttat cagttattat tgtaatattt gatactaagt aacataactt tgtctaacta
     6781 tgataaaata gttatacaat aaaggtcttg taaggagtga ataatgtgtg gctaataaaa
     6841 tcagatttgt caaatctaac attaaataat agaaaaataa attgtgtaat taatggtatt
     6901 ttaatatgaa aaaaagagaa aaagtatttg atgggctagt ttttaagcat atattaatct
     6961 tattaatact attagttttg attgatttaa caagtaccat catatatgca agtaactcta
     7021 tggaaattac ttctagaaat atgattgagg catcaaaaag agaacttgaa aactatttag
     7081 aagtaaacat atctttatta aaagctctta gtcaagatga tagattttct aatggtgaaa
     7141 catcactaat tgaaaaggga aaattattaa gtccatatca aaaggaatat aatcttttta
     7201 tgattggtat aacagataca aaaggaaatg cttcaagtac atatagggaa aatgttaact
     7261 caataaaaga taagcctagt tttgagaaag caattaaaac aaagcaagta gtagtttcag
     7321 atattgaagt cagtaatgta acaggtgaca aagtatttat catatatgta ccaattgtaa
     7381 aaaataatga gatgattggg accatttttg cttcttttta ttttcaagat gtaaataatt
     7441 taataagtag aagcaatttt gatgattcta ttaaatttct aatgatagat aaagattata
     7501 ctattatttc tcatccaaac aaaaaatatg taaatgataa aagtaagatg cttgatttag
     7561 aagggaatat tataggaacc actaaaagtg aaatcttaaa aaatataaat aaaaaatgtc
     7621 aaggtgattt tttatcttgg gataattgga gattatataa tgtaaaatat acgaacataa
     7681 agtggactaa ttggacttta gtatcaaaat gtaatatatt taagaacttt aaaaatctaa
     7741 tagttaattt tatgataaag ttgtattttt atatagtaat atttatgata ttgtggaaat
     7801 taagtaatgc taaattaata gaacaactta aaaaattggc ttattatgat tctttaagtg
     7861 gaataaaaaa taaagaaaag tttagaaaag attctatgta tattttaaag aattattatc
     7921 aagataattt ttacttagta caattggatg tcaataaatt taaatatata aatgagatgt
     7981 ttggatatgc tgaagggaat aagatattaa ttcatatatc acaagtttta aataataata
     8041 caaataaata tgaaatttgt gctaggatgg ataatgacca ttttatattg ttgattgctt
     8101 gtaacactga agatgaactt ttaaatagat tatctaaaat aaataaagaa atttgtaact
     8161 taaatactac taactcatct aagtataaaa ttgttatgtc aagtggaata tataagatta
     8221 ctaaaaagga tgatattaaa aagattgact tgttaattga tagagcaaat attgcagcaa
     8281 aaagtaaaaa ggaaaaatat gaacattcat attcattttt taatgaagat acaagaaatc
     8341 gattgtacaa ggaaaaacga ttggaagata atatgaataa agctttagaa aaaggtgaat
     8401 ttatagtata ttatcaacct aagtatagtt tagatgatgt aaatgaaatt gaaggagcag
     8461 aagccttgat aagatggaat agtccagaat ttgggtttat ttcacctata gattttgtac
     8521 ctttatttga aaagaatggt tttattgtaa atattgatat gttcgtgttt gaagaagtat
     8581 gcaaaacatt aaacaagtgg ataaataaag ggtatactcc tgtaccaatc tctgtaaata
     8641 tgtctagagt tcatttatat agggataatt ttatagaaaa tataactgat ttgataagta
     8701 aatacaatat atctcctgag tttattgaac ttgaactaac agaaagtgtt gtgtttgata
     8761 atttaaatat attgattgat ataatgaaaa agattaaaga gataggattc cttatatcaa
     8821 tggatgattt tggttcggga tattcatctt taaatctgtt aaaagacctt tcttttgata
     8881 ttttaaaatt agatagagga tttttaatcg aaacaacaga tacaaaacgt ggaaaaatta
     8941 ttatttctaa gattgtggaa atggcaaaag cgattgatat taaagtaatc tgtgagggcg
     9001 tagaaactta tgaacaagta gagttcttga aagagatagg ttgtgataag gttcaaggat
     9061 acttatttgc taaacctatg gtattagatg aatttgaaaa gcacttaaac tttaaatttg
     9121 attaatcatt aataattatt agactatgag ataccctaac aacttactta caaatatgta
     9181 agttaattgt tagggtattt ttatggttaa agtagtatat tcacgatatt atggtattgt
     9241 agtaaaaata aaaaattatg gagaatataa aatgaacata ttgagtatta gaaatatatc
     9301 aaaaacatat tgtggaaata ttccatttaa agcattagat aaggttagtc taaatataga
     9361 aaagggagaa tttgtgtctg ttatgggacc atctggaagt ggtaaatcta ctcttttaaa
     9421 tataatatct acagttgata gacagagtga aggtgaggtt gtattagatg gatatgatgt
     9481 aagtaaattg aaaggagaaa aacttgctga atttagaagg aaacaacttg gttttgtatt
     9541 tcaggatttt aatttgattg acactttaac tgttggcgaa aatattatgt tgccacttac
     9601 actagagggg tcaagtatca aagatatgaa tatacaaact aaaagtatat ctaaattttt
     9661 aggaatagat aaaattttag atagaaaaac ctatgaaatt tcaggaggtc aagctcaaag
     9721 gtgtgctatt gctagagcta ttataaataa acctgccata cttcttgcag atgagccaac
     9781 tggaaatctt gattcaaaat ctactgatga tgttttgaaa ttatttacta ggataaacaa
     9841 agaacaaaag gtaacaacac ttatggtaac tcatgaagca tatagtgcaa gttattcaga
     9901 tagagtaatt tttataaaag atggttgtat atatacagaa ataaaaaaat ctgaaagcag
     9961 taattctttc tattctgata tactagctgt tttgtctcaa ataggaggtg taagatagtg
    10021 gattttagaa agtttgctta taataatata gttagaaatt tcagggcata tttagcatat
    10081 tttttaagtt ctgttttttc tataatgata ttttttgttt ttgcagtgtc tatgttccat
    10141 ccaattataa caaaatctgg aatgcagagt ggttctactg catttatggc tcttatgact
    10201 tcagaattaa taatattcgg ttttagtcta ctttttatat tgtactcttt aggaaatttc
    10261 ttaaaatata gattgaaaga ttttggagta ttgatgataa ttggaatgag taacagacaa
    10321 ctgaaaaaat tgatattaat tgaaaatttg ataataggat ttttagcagt gatgttagga
    10381 atattacttg gtttatcaat ttcaaaattg tttttattgt atttaagcaa actgttttat
    10441 atgaatttaa ctgaatttta ttttccagta aaagcaatgc tattgactat tgtatcattt
    10501 atgattttgt ttttaataac aggaccaatg agcttaaaat tattgagtaa aaagagtatt
    10561 ttagagcttt tagtaggtac aaaaaagcct aaaaaagaga taaaatcttc aaagctattt
    10621 ggaataattg gtgtaatatg tcttatatta ggatatgcta tgatactatt tggcaataag
    10681 ttaaaaataa atggaggtat gtatacaaca gcactattaa ttactttggg gatattttta
    10741 tttttttctc aatttactat tttgatatta aagtatttaa aaaataaaga aaaattttat
    10801 aaaaacaaag taaatatgtt acttataagt aatttgatat ataaattaaa ggataataca
    10861 cgattgttat ttttaataac aatactttta tcggctactt tagtagcatt tacaacaact
    10921 tcaacacttg taagttctca aggagaagct tctaaaaata atttcccaat ggtttactca
    10981 tatttttcag agcataataa tgaaaaagaa tatgaagaac tacaacaaat tagagaagca
    11041 atcaaaggat atgattataa ggagttaagt tttcctgtac ttaaatataa tagggaagtc
    11101 ttactaagtg taaagaatta taacaaatta tcaaaggaat tgggattaga taaaattagt
    11161 tcaaagttag aaaaatatga agggattata attcctagta gcaattcgca agaacatata
    11221 aattctttaa aacaattaaa aaattatagt ataaaaaatt taaatataga agtaaaaaaa
    11281 tcaaaaaata aaataatttt tcctacagga ctgtttagta aagttgtagt tataagtgat
    11341 gacttataca atgaagtaaa aggtgaattt tcaaaaatta attttcatgg atttgattat
    11401 aaaaactggc aaagcagtgc gaagataact gaaaatttaa aagaaaaaca ttttaatttg
    11461 gatagagaat ataacaggtc atatttttta acattaccag atatgtattt agtagagtta
    11521 caacaaagta aaattcttca atttatgggt gtttttatag gagtgatatt atttatagca
    11581 gtttttagtt ttttatactt cagattatat acagacgaga tagaagataa tgtaaagtac
    11641 aaaaaactat caaaaatagg tctatctttt aaaaatatga ataagattgt tagtatagag
    11701 ataggtacat tgttttttat accgtatttt atagcaatta taaatagcat aatttcttta
    11761 ctgtttttaa atcaagtatt aaataatagt tttaagttag aaaatttggg tatattacta
    11821 ctgatatcat ctatatattc actttatttt tacttattaa aaaaaattta tactagaaaa
    11881 gtttggtttt ttgattaaag tggtatataa tagatatata aattaatatt taaaaagtag
    11941 ttaatattag gggagggcat ttgcacctcc cttgtaatat gtggaaaatt gaatattaga
    12001 agaaggagga gacttgatgg aaaatcaaca attattagga acagaacgaa ttagtaaatt
    12061 actactaaaa tattctatac cagctataat aggtatgttg gtaaatagtt tatataatgt
    12121 agtagataga atttttattg gaaatatacc aggggttgga ccacttgcaa taacaggtct
    12181 tggtgtaaca atgccaataa tgacaataat acttgcattt gggatgttaa taggtatagg
    12241 aactactact accatatcaa taaaacttgg gcaaggtaaa gtagaagagg caagaaaact
    12301 tataggaaat gcaatgacgt tatctgtaat aacaggaatt atcataatga tacttggaat
    12361 attatttgca aataaaatac ttacattgtt tggagcaagt gaaaatactt taatatatgc
    12421 aaaatcatat atcaatatta tattattagg tacagtagta aatcttcttt ccttttcttt
    12481 aaaccactca ataagagctg atggtagtcc taaaatttca gcaggaataa tgatagtagg
    12541 atgtttaact aatatagttt tagactggat attaatattt ggattcaatt tgggaataca
    12601 aggagctgca attgctacag taacttctca ggctcttaca gctattttaa ctatagggta
    12661 ttatataagt ggaaaatcaa acttacgatt cagtaaatct aatctaaaat tagataaaaa
    12721 gctaataaaa gcagtgtttg caataggtat gtcgccattt gcgatgcaac ttgcagcaag
    12781 tttagttcaa gtaatatcaa atattgcact taaaactcat ggtggagatt tagctatagg
    12841 ggctatggct actatttctt ctattgcaat ggtatttttg atgcctatct ttggaataaa
    12901 tcaaggagcg caacctatta taggtttcaa ttatggagct gaaaaatatg atagggttaa
    12961 aaaagcatat ttaggttcat tagtagtagc tactataatt ttatgtatgg gtatggtagt
    13021 agttatgctt ttcccagaag ctattatagg tatatttaat aaagaccctg aacttatgaa
    13081 tatatctgtt aatggattaa gaatatactt acttatgttg ccaattgtag gcttatcagt
    13141 tacaggaaca aactttatac aatctatagg aaaagcaaaa atggctatgt tattgagcct
    13201 tttaagacaa gtaattttat tgataccagc agtattaatt cttcctacat tcttaggctt
    13261 acagggtgta tggacagctc aaccagtgtc tgattttata gctacagtaa ttacaggtat
    13321 agttgtattt agagaattaa aaagatatac tcctaaaact gaaaaattaa atgaaaatga
    13381 aagactaaac gagataacta ctgagtaaaa atataaattc agataattga gcaagaaatt
    13441 gcaaaaaata cgattatatg ataaaataaa accgctgtca aggataagag aaattctaat
    13501 tatggagtga ttttatggaa tttacaaaga aaagcaaatt aaaaaatatg tatgtaaaca
    13561 tgaatgtagc tagaatgttg tctaagataa atgagtataa gggtagacaa ctgctttaca
    13621 aaaaacaacc aaaagaaatt ttagaaaact tagaaaaaaa atctttagta gattgttctg
    13681 aatctacata cattggaaat caaagagata gtagtaattt taatctagaa aagttgataa
    13741 gtaatgaagt tacacctaga agtagagagg aattgagtat tgttgaatac agagatgtgg
    13801 taaaaactat taacagtgct tatgagagta ttcccatcag ttcacaaacg attcttgagc
    13861 ttcatggata cttatataaa ttttcatcta ctagaggagg aagttataag tcagataatg
    13921 attttattga acacaattta aaacctagcg aatttataag tattgattca agtgtcaata
    13981 aagttgaaaa agctgtagaa gaaatatgtg aagcttataa tacattgata gaggaagatg
    14041 aaattgatat attaatatta atttctgcat ttgtgttaga tttcatattg atacatccat
    14101 tcaaagaagg taatataaaa atggcgagag tacttatcct attgttacta aataaaaatg
    14161 ggtatgaagt tggaagatat ataagtttag gaaagatatt tgatgatagt tcatatgaat
    14221 attatagtaa tttaaattat ttgaaagcct caataggtag tgaaaaagca gatatgaatg
    14281 catggattga gtatttttta gagacgatat taactgcata tgagaagctg gatgatagtt
    14341 taaatatatc tgataagaaa agacaaacaa aaactagtag aatagagaaa ataataaatt
    14401 ctacccttgg atattttaca aaagaagata taagagattt atgtcctgac attccagaac
    14461 ctactataaa tagagttttt aataatctaa gaaaacaaga taagatagaa gttgttgcaa
    14521 gaggaagaag tgcaaaatgg aagaaaaaat attaaaaaat gtttagataa ctaaaatggt
    14581 atagtgtttt acactatacc atttttaatg tcattagcat gagcaataat ttaacaactt
    14641 aagcaatatc aattttattt attgattatt tttgaactga aaaatggcgt tatatggtat
    14701 acctcagtgg taattaattt tggatgagaa gatataatat tacagaatat attaggagta
    14761 tatgtagaaa aaatataata aaagtgtgag gcttagtaat taacaaatta tgtggcttca
    14821 cactttttta taaaataaag atacttagca gaataatatt ttagttgata tgtttataag
    14881 atggtacttg tttgggtaaa agtataaata gtaaaaaagt agaagtaatg tgtataaaaa
    14941 cataagttac attctgtaat aatagttatt ttataattca acaaaagaat agaatgggta
    15001 tatttgtgaa ataataatat aaaataatct taggagtgag cttatgaaga aaaaattatt
    15061 atatataaat gtaaattcaa aacctgaaga cttatcttca agtaaaactg tagcaagaaa
    15121 atttataaat aaatttatgg aaaaaaataa agattttgag gttgaagaga tagaccttta
    15181 taaagaacat ataccaagac ttgagtatca gtactttgaa aaaagaaata gcatagttag
    15241 tgaagaaaat gctaaaaatt tagacgataa ggatagaaaa gagcttacaa agattagaaa
    15301 tctttgtgac caatttgtga gtgcaagtgt atatgtaata gcagcaccta tgtggagttt
    15361 atcttttcca gcaccactta aagagtatat agattgtata attcaagatg gaaaaactat
    15421 atcttttgaa ggcaatgata aaccacaagg aattttaaat gatattgata gaagtatggt
    15481 atatatacag tcttctggtg gacacatacc atgggtgttg aagccagtta tgaataaagg
    15541 gttaaattat gtggaaagta ttatgaaatt tataggaatt aaaaaatttg atgaattgtt
    15601 agtagatgga actggaacta gtgaagaaga aagacaagct gcaatagaaa aagcatctga
    15661 aaaaatagat ggtataatag atgggatgaa attttaatta taatatttta ttaaaaatat
    15721 aaaggaaaaa cctcttttaa tgtagaaact tatattgaag gaggcgagaa tcatgcgaga
    15781 agaaaaaagt aatgaaaagt atgattgtta ttggtgtaat caagagaata atttctgtgt
    15841 agaaataaaa gataatatag tcatgataga tgatggcact ggtacgttaa aacaagccgt
    15901 tttcataggg tataaacaaa tccaaattaa tttaaactgt tcacattgtc aaaacttaaa
    15961 tagaataaaa ttaaatttgt agaattttaa aatggaaatt attgaaggaa ttaaaaatag
    16021 aaagtgttaa tatttagact gaaaattata ttttatacaa t
//
LOCUS       DBGJFF010000086        15978 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.087, whole genome shotgun sequence.
ACCESSION   DBGJFF010000086 DBGJFF010000000
VERSION     DBGJFF010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15978
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.087"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(75..2141)
                     /gene="fusA"
                     /locus_tag="ACOIAF_002654"
     CDS             complement(75..2141)
                     /gene="fusA"
                     /locus_tag="ACOIAF_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HGZ2669658.1"
                     /translation="MARKFPLERTRNIGIMAHIDAGKTTTTERILFYTGQTHKIGETH
                     EGASQMDWMEQEKERGITITSAATTASWKDHRINIIDTPGHVDFTVEVERSLRVLDGS
                     VAVFCAKGGVEPQSENVWRQAETYGVPRIAFVNKMDILGADFYNVVSMMKSRLNSNAV
                     PMQLPIGKEDSFIGIIDLLKMDAVIYKDDLGVEMEETDIPEDMKELAAEWREKLVESV
                     AETDEELMMKYLEGEELTIDELKVAIRKATIACEMNPVFCGTAYRNKGVQLVIDAVLD
                     YLPAPTDIPAIKGILADGEEAERHSSDEEPFSALAFKIMTDPFVGKLAFFRVYSGTLE
                     SGSYVLNATKNKRERIGRILQMHANTREEITKVYAGDIAAAVGLKDTTTGDTLCDPAN
                     PIILESMEFPEPVISVAIEPSSKAAQEKMGIALQKLAEEDPTFTVKTDQETGQTIISG
                     MGELHLEIIVDRLLREFKVEAKVGAPQVAYRETITQPVDVEYKYSKQSGGRGQYGHVK
                     IRVAPQEPGEGYKFTNKTVGGSVPKEYVGPVDMGIQGAMQSGIVAGYPVVDVAVELYD
                     GSYHEVDSSEMAFKMAGSMAFKDAMKKGNAVLLEPYFKVEVVTPEDYMGDVMGDLNSR
                     RGLIQGMEARSGAQVINAFVPLSEMFGYSTDLRSSTQGRATYTMIFDHYEQVPASVAK
                     KIAEGK"
     gene            complement(2190..2660)
                     /gene="rpsG"
                     /locus_tag="ACOIAF_002655"
     CDS             complement(2190..2660)
                     /gene="rpsG"
                     /locus_tag="ACOIAF_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HGZ2669659.1"
                     /translation="MPRKGNIPKREVLPDPMYGSKVVTKLINNLMVDGKKGKSQRIVY
                     DAFAIVAEKTGEEALEVFNKAMDNIMPVLEVKARRVGGANYQVPIEVRPERRQTLGLR
                     WLVKYTRARGEKGMVEKLAKEIMDAANNTGASVKKKEDTHKMAEANKAFAHYRW"
     gene            complement(2784..3206)
                     /gene="rpsL"
                     /locus_tag="ACOIAF_002656"
     CDS             complement(2784..3206)
                     /gene="rpsL"
                     /locus_tag="ACOIAF_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HGZ2669660.1"
                     /translation="MPTINQLVRKSRKALEKKSTAPALQKGYNSLNKKVTDASAPQKR
                     GVCTSVKTVTPKKPNSALRKVARVRLTNGIEVSAYIPGEGHNLQEHSVVLIRGGRVKD
                     LPGVRYHILRGTLDTAGVDKRRQSRSKYGAKRPKEAKK"
     gene            complement(3495..6980)
                     /gene="rpoC"
                     /locus_tag="ACOIAF_002657"
     CDS             complement(3495..6980)
                     /gene="rpoC"
                     /locus_tag="ACOIAF_002657"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HGZ2669661.1"
                     /translation="MFELNNFESIKIALASPEKIRQWSRGEVKKPETINYRTLKPEKD
                     GLFCERIFGPQKDWECHCGKYRRVRYKGVVCDRCGVEVTKSKVRRERMGHIELAAPMS
                     HIWYFKGIPSRMGLLLDMSPRSLEKILYFASYVVVDPGETGLNEKQLLTEKEYRTALE
                     KYGYTFTVGMGAEAVKTLLQNIDLEQQSKDLRAELKDSTGQKKVRTIRRLEVVEAFKK
                     SGNKPEWMILDAIPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLELGA
                     PDIIVRNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSDMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPELKFYQCGLPKKMALELFKPFVMDKLVKEGYAHNIKSAKSIVEK
                     VKPEVWDVLEDVIKSHPVLLNRAPTLHRLGIQAFEPILVEGKAIKLHPLVCTAYNADF
                     DGDQMAVHVPLSVEAQAEARFLMLSVNNILAPKDGSPITTPSQDMVLGCYYLTIEAQD
                     GAKGTGMVFKDFNELLLAYYNKSVHLHALVKLKVTLEDGRSSLVESTVGRFIFNENIP
                     QDLGFVDRKENPFALEVDFLADKKSLGKIIDKCFRKHGNTETAELLDYIKALGFKYST
                     LGGITVAVDDMSVPEEKKVFIAEAEAKVDKYEKAYRRGLISDEERYEKVIETWTETTD
                     KVTDALMGGLDRLNNIYIMAHSGARGSKNQIRQLAGMRGLMANASGKTVEIPVKSNFR
                     EGLSVLEYFTSSHGARKGLADTAIRTAESGYLTRRLVDVSQDVIVREIDCGTEDTTEI
                     YAIKEGNEVIEEIYDRIVGRYTIDPILNPETGEVIVEADSMIQEDEAETIVALGIEKI
                     RIRTVLNCKTNHGVCSKCYGRNLATGKEVNIGEAVGIIAAQSIGEPGTQLTMRTFHTG
                     GVAGADITQGLPRVEELFEARKPKGLAVITEVSGRVEIDETGKRKEVNVIPEEGETQT
                     YVIPYGSRLKVKQGQMLEAGDPLTQGFINPHDIVRVNGVKGVQEYIVKEVQRVYRLQG
                     VDVNDKHIEVIVRQMLSKVKVEDPGDTDLLPGGYEDVLTFNECNKDAIDKGLRPAVAK
                     RVLLGITKASLATDSFLSAASFQETTRVLTEAAIKGKEDHLIGLKENVILGKLIPAGT
                     GMKKYRNIAVEKIED"
     gene            complement(7022..10738)
                     /gene="rpoB"
                     /locus_tag="ACOIAF_002658"
     CDS             complement(7022..10738)
                     /gene="rpoB"
                     /locus_tag="ACOIAF_002658"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HGZ2669662.1"
                     /translation="MPHPVTIGKRTRMSFSKIKEIADVPNLIEIQVDSYEWFLKEGLK
                     EVFDDISPIEDYTGNLILEFVDYSLDDKPKYDIEECKERDATYCAPLKVKVRLINKET
                     GEIKEQEVFMGDFPLMTERGTFVINGAERVIVSQLVRSPGVYYAEERDKTGKRLISST
                     VIPNRGAWLEYETDSNDVISVRVDRTRKQPVTVLLRALGIGTDAEIIDLLGEDERLSA
                     TLEKDNTKTVEEGLVEIYKKLRPGEPPTVESASSLLNALFFDPKRYDLAKVGRYKFNK
                     KLALCYRIMNKISAEDIINPETGEVFVKAGEKISYDLAKAIQNAGINVVNLLMDDDKK
                     VRVIGNNFVDIKSHIDFDIDDLNIKEKVHYPTLKEILDGYSDEEEIKEAIKSRIKELI
                     PKHILLDDIIASISYEFNIFYNIGNIDDIDHLGNRRIRSVGELLQNQVRIGLSRMERV
                     IKERMTVQDMEAITPQALVNIRPVSAAIKEFFGSSQLSQFMDQTNPLSELTHKRRLSA
                     LGPGGLSRERAGFEVRDVHHSHYGRMCPIETPEGPNIGLINSLGTYAKINEFGFIESP
                     YRKFDKETSTVTDEIHYLTADEEDLFVRAQANEPLTEDGKFVNHRVVCRTVNGAVEMV
                     PESRVDYMDISPKQVVSVATAMIPFLENDDANRALMGANMQRQAVPLVRREAPIIGTG
                     IEYRAAKDSGAVVVARNSGIAERVTADEIIIKREDGNRDRYNLLKFKRSNSGTCINQT
                     PIINKGDQIIKGDVIADGPATDLGEVALGRNCLIAFMTWEGYNYEDAILINERLVKED
                     RLSTIHIEEYECEARDTKLGPEEITRDIPNVGDSAIKNLDDRGIIRIGAEVDSGDILV
                     GKVTPKGETELTAEERLLRAIFGEKAREVRDTSLKVPHGESGIIVDVKVFTRENGDDL
                     SPGVNELVRCYIAKKRKIKVGDKMAGRHGNKGVISRVLPEEDMPFMENGTPLDIILNP
                     QGIPSRMNIGQVLEVHLGLAAKTLGWYVATSVFDGANEYDIMDALEEAGYPRDGKLTL
                     YDGRTGESFDNRITVGYMYYLKLHHLVDEKLHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAHILQEILTVKSDDVVGRVRTYEAIVKGENIPEPGIPESFKVLIK
                     ELQSLCLDVKVLTDEDQEIEVRESVDEDDTIGEFELDVVNHMGEVEESNIIEEIEDDF
                     AENAEDEDIENLEEFTEDDLFEEEIDFDSDDFDM"
     gene            complement(11209..11997)
                     /locus_tag="ACOIAF_002659"
     CDS             complement(11209..11997)
                     /locus_tag="ACOIAF_002659"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HGZ2669663.1"
                     /translation="MEKLQGKIAVVTAATKGIGLASAEILAKNGATVYLAARSEELAN
                     EVINKISAEGGCAKFVYFNAREEETFTSMIEEVVKKEGKIDILVNNFGSTNPSLDKDL
                     VTGDTDNFFDTVNTNLKSVYLPCKAAIPHMIKNGKGSIVNISSIGSVLPDLSRIAYCV
                     SKAAINSLTQNIATQYAKDNVRCNAVLPGLIATKAALDNMSPEFIKEFLKHVPLNRIG
                     EPDDIAKAVLFYASDDSSFITGDLLEVAGGFGLPTPQFADNILG"
     gene            complement(12388..12753)
                     /gene="rplL"
                     /locus_tag="ACOIAF_002660"
     CDS             complement(12388..12753)
                     /gene="rplL"
                     /locus_tag="ACOIAF_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002842856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HGZ2669664.1"
                     /translation="MTIEQILEAIENMKVLELNELVKAAEEKFGVSASAPVMVAGAAA
                     GGPAAEEKTEFDVVLTDVGSSKVGVIKAVREITGLGLKEAKEVVDNAPKTVKEGASKE
                     EADQIKEKLEAAGAKVEVK"
     gene            complement(12811..13317)
                     /gene="rplJ"
                     /locus_tag="ACOIAF_002661"
     CDS             complement(12811..13317)
                     /gene="rplJ"
                     /locus_tag="ACOIAF_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HGZ2669665.1"
                     /translation="MRKAIEIKSEVVSEIVEKLQKSSAAVVVDYKGLTVEEVTELRKQ
                     MREAGVDYKVYKNTLVRRAAKEVGIEQFNDELLVGTNAIAFGYDDPVAPARILKGFMD
                     SHPKMKLKMGIVEGAFYDESKIVEMANIPSREVLIAKLLGSLKAPVSNFAYLIDAIAK
                     KAEGQEEA"
     misc_feature    complement(13360..13488)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(13540..14238)
                     /gene="rplA"
                     /locus_tag="ACOIAF_002662"
     CDS             complement(13540..14238)
                     /gene="rplA"
                     /locus_tag="ACOIAF_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HGZ2669666.1"
                     /translation="MAKKGKRYAGALEKVDRTKFYDASEALTLVSDIAGAKFDETVEA
                     HIKLGVDSRHADQQVRGAVVLPHGTGKTKRVLVFAKGEKAKEAEQAGADFVGAEELVQ
                     KIQGENWFDFDIVVATPDMMGVVGRLGRVLGPKGLMPNPKSGTVTFDVAKAIDEIKAG
                     KVEYRLDKTNIIHVPVGKVSFGGEKLTENFTALMDAIIKAKPAAAKGQYLRSITVASS
                     MGPGVKINPARTAE"
     gene            complement(14309..14734)
                     /gene="rplK"
                     /locus_tag="ACOIAF_002663"
     CDS             complement(14309..14734)
                     /gene="rplK"
                     /locus_tag="ACOIAF_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HGZ2669667.1"
                     /translation="MAKKVIGQIKLQIPAGKATPAPPVGPALGQHGVNIMGFTKEFNA
                     KTADQAGMIIPVVITVYQDRSFSFITKTPPAAVLIKKALNLKSGSGEPNKKKVAKMTS
                     AQVREIAELKMPDLNAASVEAAMSMIAGTARSMGVVIED"
     gene            complement(14773..15315)
                     /gene="nusG"
                     /locus_tag="ACOIAF_002664"
     CDS             complement(14773..15315)
                     /gene="nusG"
                     /locus_tag="ACOIAF_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HGZ2669668.1"
                     /translation="MSELQEASWYVVHTYSGHENKVKATIEKAVKTRGMEDCIRQVVV
                     PTEEVVETTKTGKEKTRQRKVYPSYVLVKMIITDESWYVVRNTKGVTGFVGPGSKPVP
                     LSEDEVKAMGIDTTDPKVVNSDIDFEIGDTVKVSQGPFSGQIGNIEEIDLENREVKVC
                     INAFGKRTLFVIELEGIEKI"
     gene            complement(15335..15556)
                     /gene="secE"
                     /locus_tag="ACOIAF_002665"
     CDS             complement(15335..15556)
                     /gene="secE"
                     /locus_tag="ACOIAF_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HGZ2669669.1"
                     /translation="MAAQKNDGAKTKKRFSLFGYLKETKQELKRVTWPTKKELFKNTS
                     IVLTVVISCTILVWGIDTILSGALALLLK"
     gene            complement(15582..15731)
                     /gene="rpmG"
                     /locus_tag="ACOIAF_002666"
     CDS             complement(15582..15731)
                     /gene="rpmG"
                     /locus_tag="ACOIAF_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014262850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HGZ2669670.1"
                     /translation="MRVKVTLACTECKQRNYNTTKNKKNNPDRIELQKYCRFCKKHTT
                     HKETK"
BASE COUNT         4484 a         3249 c         1987 g         6258 t
ORIGIN      
        1 atcttgtgct agatgagaga tatctctcat ctagctgatt attttttata tttatattat
       61 taattatttt taaattattt tccttcagct attttcttag caactgaagc tggaacttgc
      121 tcatagtgat cgaatatcat tgtgtaagtt gcacgacctt gagttgaaga tcttaggtct
      181 gttgagtatc caaacatttc tgaaagtgga acgaatgcat ttataacttg tgcaccactt
      241 ctagcttcca taccttggat taatcctctt cttgagttta agtcacccat aacatctccc
      301 atgtaatctt ctggagtaac aacttctact ttgaagtaag gctctaaaag aactgcatta
      361 cctttcttca tagcatcttt gaatgccatt gaaccagcca tcttaaacgc catctcagaa
      421 gagtcaacct catggtaaga accatcatat aactcaacag caacgtctac aactggatat
      481 ccagcaacta taccagattg catagctcct tgtataccca tgtcaactgg accaacatat
      541 tcttttggta ctgatccacc aacagttttg tttgtgaatt tgtatccttc acctggctct
      601 tgaggagcaa ctctaatctt aacgtgaccg tattgtcctc taccacctga ttgcttagag
      661 tacttgtatt caacatcaac tggttgagtt atagtctctc tatatgcaac ttgtggtgca
      721 ccaactttag cttctacttt aaattctctt aataatctat ctactattat ctctaagtgt
      781 aattcaccca taccagatat tatagtttgt cctgtttctt ggtcagtctt aactgtgaaa
      841 gttgggtctt cttcagctaa tttttgaaga gctataccca ttttttcttg agctgcttta
      901 gaacttggtt ctatagcaac agatataact ggctcaggga attccataga ttcaagtatt
      961 attggattag cagggtcaca taatgtgtct ccagtagtag tatcttttaa tcctactgct
     1021 gctgctatat ctccagcata cactttagtt atttcttctc ttgtattagc atgcatttgt
     1081 aatatacgac caattctttc tctcttattc ttagttgcat ttaaaacata agaacctgat
     1141 tctaatgttc cagaataaac tctgaagaac gctagttttc caacgaatgg gtcagtcatt
     1201 attttgaatg ctaatgcaga gaatggctct tcatcagaag aatgtctttc agcttcttct
     1261 ccatctgcta atataccttt tatagctggt atatcagttg gcgctggtaa gtaatctaaa
     1321 actgcatcta ttactaactg aacacctttg tttctataag ctgtaccaca gaaaactggg
     1381 ttcatttcac aagctatagt agcttttctt atagcaactt ttaattcatc tatagtaagt
     1441 tcttcacctt caaggtattt catcattaat tcttcatcag tttcagctac tgattcaact
     1501 aatttttctc tccactcagc agctaattct ttcatatctt ctggtatatc tgtttcttcc
     1561 atttcaaccc ctaggtcgtc tttgtatata acagcatcca tttttaataa gtctattata
     1621 cctataaatg aatcttcttt acctattggt aattgcatag gaactgcatt agaatttaat
     1681 ctagatttca tcatgcttac aacattgtag aaatctgcac ctaatatatc cattttattt
     1741 acaaaagcta ttctaggtac tccataagtt tcagcttgtc tccatacatt ttctgattga
     1801 ggctcaaccc caccttttgc acagaataca gcaactgaac cgtcaagaac tcttagagat
     1861 ctttcaacct caactgtgaa gtccacgtgt ccaggagtat ctattatgtt tattctatgg
     1921 tctttccatg aagcagtagt agcggcagaa gttattgtta tacctctttc cttttcttgc
     1981 tccatccagt ccatttgaga agctccttca tgagtttctc ctattttatg agtttgccca
     2041 gtataaaaca ggattctttc tgtagtagta gtttttcctg catctatatg agccatgatt
     2101 cctatattcc tagttctttc caaaggaaac tttctagcca tggttatcct ccttatgata
     2161 tcctcaattt tataagatat ctatatatcc taccatctgt aatgagcaaa tgctttatta
     2221 gcttctgcca ttttatgagt atcttctttt ttcttaactg aagctccagt gttgttagct
     2281 gcgtccatta tctctttagc tagcttttca accattcctt tttctccacg agctctagta
     2341 tattttacta gccatcttaa acctaatgtt tgtcttcttt caggtctaac ttcgattggc
     2401 acttggtagt ttgccccacc aactcttctt gcttttactt ctaaaacagg cattatatta
     2461 tccattgctt tattaaatac ttctaaagct tcttctcctg tcttttcagc aacaattgcg
     2521 aaagcatcat aaactattct ttgagacttt cctttttttc cgtctaccat tagattattt
     2581 attaatttag taactacctt actcccatac ataggatctg gtaaaacttc tctttttgga
     2641 atattacctt ttcttggcat ttcgcttccc tccttaatta tttaacttaa atcataggta
     2701 ctcgacataa atcatttaat gccgtgatgc cgtaaatata tatatattta aagcaccgaa
     2761 ctataatcaa tcattaattg tttttacttt ttagcttctt taggtctctt cgcaccgtat
     2821 ttagatcttg attgtcttct cttatcaaca cctgctgtat ctagagtacc tcttagtata
     2881 tggtatctaa cccctggaag gtcttttact cttcctcctc ttataagaac aacactatgc
     2941 tcttgtaagt tgtgtccttc tcctggtata taagcagaaa cttctatacc attagttaat
     3001 ctaactctgg caacttttct taaagctgag ttaggcttct taggagtaac tgtttttact
     3061 gaagtacaaa cccctctttt ttgtggggca ctagcgtcag ttactttctt atttaaagag
     3121 ttgtaacctt tttgtaatgc tggagcagta gatttcttct ctagcgcttt tctactttta
     3181 cgaactaatt ggttaattgt tggcatcatc tgcacctcct tccaaatttt cgatcttatc
     3241 agtgaccgcc aactacatca atatgaatat tcatactgac tgattgtaac attcgttact
     3301 tacaatttaa tgtctataac acataaatat gtgatattag cacttttcta gcttgatgct
     3361 ttttagttct catttattgc catctaaaag tcactaagaa cttgttcaag cccttagtgt
     3421 acttttaaac aacacacttt actattttaa caccacaaat aatggttgtc aagatttctt
     3481 tactctatat gctattaatc ttcaattttt tcaacagcta tatttctata tttcttcatt
     3541 cctgttcctg ctggtattaa cttacctaat ataacatttt ctttaagtcc tattaagtgg
     3601 tcttctttac cttttattgc tgcctctgtt aatactcttg ttgtttcttg gaaagaagct
     3661 gctgataaga atgaatcagt tgcaagagat gctttagtta taccaagtaa aactctttta
     3721 gcaactgctg gtcttagacc tttatctata gcatctttat tacattcatt gaatgttaat
     3781 acatcttcat atccacctgg caataaatct gtatctcctg ggtcttcaac ttttacttta
     3841 gatagcattt gtcttactat aacttctata tgcttatcat taacatcaac cccttgaagt
     3901 ctatacactc tttgaacttc tttaactata tattcttgaa ctcccttaac accatttact
     3961 cttactatat catgaggatt tataaatcct tgtgttagag ggtctccagc ctctagcatt
     4021 tgaccttgct taacttttaa tctagaacca tatggaatta catatgtttg agtctcgcct
     4081 tcttctggta ttacatttac ttcttttctc tttcctgttt catcaatttc aactctacca
     4141 gatacttcag ttattacagc taatcctttt ggttttctag cttcaaataa ttcttcaacc
     4201 cttggtagac cttgagttat atcagctcct gcaactcctc cagtatggaa tgtacgcatt
     4261 gtaagctgag tacccggctc accgatagat tgagctgcta ttataccaac tgcttcacct
     4321 atattaactt cttttcctgt tgccaagttt ctaccataac acttagaaca aactccatga
     4381 ttagttttac agttaagaac tgttcttatc ctaatttttt caattcctaa agctactata
     4441 gtttctgctt catcttcttg tatcattgaa tcagcctcaa ctataacttc tccagtttca
     4501 ggatttaata tagggtctat agtatatctt cctacaattc tatcatatat ctcttctata
     4561 acttcatttc cttcttttat agcataaatc tctgtagtat cttctgttcc acaatctatt
     4621 tcccttacaa taacatcttg acttacatca acaagtcttc ttgttaaata tccagattca
     4681 gctgtacgta tagctgtatc ggcaagacct tttctggcac catgtgaaga tgtaaagtat
     4741 tctagtactg ataaaccttc acggaaatta gatttaactg gtatctcaac tgttttacca
     4801 gatgcatttg ccataagacc acgcatacct gccagctgtc taatttggtt tttagaacct
     4861 ctggctcctg aatgtgccat tatatatata ttatttaatc tatctagtcc acccataaga
     4921 gcatcagtaa ctttatcagt tgtttctgtc catgtctcta taactttttc atatctttct
     4981 tcatcagaga ttagacctct tctgtatgct ttttcatact tatcaacttt agcttctgcc
     5041 tcagctataa atactttctt ttcttctggg acactcatat catcaacagc aactgttatg
     5101 ccacctaatg tagaatattt aaatcctaga gcttttatat aatctagtaa ttcagctgtt
     5161 tctgtatttc cgtgttttct aaagcattta tctattattt taccaagaga ttttttatct
     5221 gctaagaaat caacttcaag tgcaaatgga ttttcttttc tatctacaaa acctaagtct
     5281 tgaggtatat tttcattaaa tataaatcta ccaacagtac tttcaaccaa tgaacttctt
     5341 ccatcttcaa gtgttacctt aagtttcact aatgcatgta gatgaactga tttattgtaa
     5401 taagcaagta atagttcatt aaagtcttta aataccatac ctgttccttt agctccatct
     5461 tgggcttcta ttgttagata atagcaacct aaaaccatat cctgagatgg agtagttata
     5521 ggtgaaccat ctttaggagc aagtatatta tttacagaaa gcattaagaa tcttgcttct
     5581 gcttgtgctt ctactgataa aggtacatga actgccattt ggtcaccatc aaagtctgca
     5641 ttataagctg tacatacaag aggatgtagt tttatagcct taccttcaac taagattggt
     5701 tcaaatgctt gtatacctaa tctatgtaga gtcggtgcac ggttaagaag aactggatga
     5761 ctttttataa catcttctaa aacatcccaa acttctggct taactttttc tactatagat
     5821 ttcgcacttt ttatattatg tgcataacct tctttaacta atttatccat aacaaatggc
     5881 ttgaataatt ctaatgccat cttctttgga agtccacatt gataaaattt aagttctggt
     5941 ccaacaacta taacagaacg tcctgagtag tcaacacgct taccaagtaa gttttgacgg
     6001 aaacgacctt gcttaccttt taacatatct gataaagatt taagtggtct atttccaggt
     6061 cctgttacag gtctacctct tctaccatta tctatcaatg catcaacagc ttcttgaagc
     6121 attctttttt catttcttac aattatatct ggagctccaa gctctaataa tcttttaagt
     6181 ctattgtttc tatttataac tcttctatat aaatcattta ggtctgaagt cgcaaatctt
     6241 ccaccatcaa gttgtaccat tggtcttaaa tctggtggta ttactggtat tgcatctaaa
     6301 atcatccatt ctggtttatt tccagacttt ttaaatgcct ctacaacttc taatcttctt
     6361 attgttctaa ctttcttctg ccctgtacta tcttttaact ctgctcttag gtctttactt
     6421 tgttgctcta aatctatatt ttgtagtaat gtctttacag cttcagcacc cattcctaca
     6481 gtaaaagtat atccatactt ttcaagagca gttctgtatt ctttttctgt aagcaattgt
     6541 ttttcgttta atccagtctc tcctggatca actaccacat atgaggcaaa gtataatatt
     6601 ttttctaacg atcttggtga catatcaagt aaaagtccca ttctacttgg tatacctttg
     6661 aagtaccaga tgtgagacat aggagcagct agctctatat gtcccattct ctctcttctt
     6721 acttttgatt tagttacttc tactccacat ctatcacaaa ctacaccttt atatctaact
     6781 cttctatatt taccacagtg gcactcccag tctttttgtg gtccaaatat tctttcacag
     6841 aaaagaccat ctttttctgg ttttaaagta cggtaattta tagtttctgg ctttttaact
     6901 tctcccctag accattgtct tattttttct ggagaagcca atgctatttt tatcgactcg
     6961 aaattgttta attcaaacaa ggagttctct cccttctatt tgagatttac attaattaaa
     7021 tttacatatc aaagtcatca ctatcaaaat ctatttcttc ttcaaataaa tcatcttcag
     7081 tgaattcttc taaattctct atatcctcat cttctgcatt ttctgcaaaa tcatcttcaa
     7141 tttcttctat tatattactt tcttcaactt ctcccatatg atttacaaca tctagctcaa
     7201 attcacctat tgtatcatct tcatccacag attctcttac ttctatttct tggtcttcat
     7261 ctgtcaatac ttttacatct aagcataaac tttgaagttc ttttataaga accttaaatg
     7321 attctgggat tcctggttca ggtatatttt cacctttaac tatagcttca tatgttctaa
     7381 cacgacctac aacgtcatca gacttaacag taagtatttc ttgaagtatg tgagcagctc
     7441 catatgcttc cagagcccaa acctccatct ctccaaatct ttgtccacca aattgtgctt
     7501 taccacctag tggctgttga gtaactaatg agtatggtcc agtacttctt gcatgtagtt
     7561 tttcatctac taagtgatgt agtttcaagt agtacatata tccaacagtt attctgttgt
     7621 caaaagactc acctgttcta ccatcatata aagttaattt accatctcta ggatacccag
     7681 cttcttcaag agcatccatt atgtcatact cattagcacc atcgaatact gatgttgcta
     7741 cataccatcc taatgtctta gcagcaagtc ctaaatgaac ttctaaaacc tgaccaatgt
     7801 tcatacgtga tggtatacct tgtgggttaa gtattatgtc tagtggtgtt ccattttcca
     7861 tgaatggcat atcttcttca ggtaatactc ttgatataac ccctttatta ccatgacgac
     7921 cagccatttt atctccaact tttatttttc tctttttagc tatatagcat cttactaatt
     7981 catttactcc tggagataaa tcatctccat tttctctagt aaatactttt acatcaacta
     8041 ttataccaga ttcaccatga ggcactttaa gtgaagtatc tctaacttct ctagcctttt
     8101 ctccaaatat tgcacgaagt aatctttctt cagcagtaag ttcagtttct ccttttggag
     8161 ttactttacc aactaatata tcacctgagt ctacttctgc acctattctt attatacctc
     8221 tgtcatctaa gttcttaatt gcactatctc caacattagg tatatctcta gttatttcct
     8281 ctggccctag ttttgtatct ctagcttcac attcatattc ttctatatga attgttgata
     8341 atctatcttc tttaactaat ctttcattta ttaatatagc atcctcgtag ttataacctt
     8401 cccaagtcat gaatgctata agacagtttc ttccaagtgc aacttctcct aaatcagttg
     8461 ctggaccatc tgctataaca tcacctttta ttatctggtc acccttattt attataggtg
     8521 tttgatttat acaagtacct gagtttgaac gtttaaactt aagtagatta tatctatctc
     8581 tgtttccatc ttctcttttt ataattattt catctgctgt tactctttct gctataccag
     8641 aatttctagc aacaacaact gctccagagt cttttgctgc tctgtattct ataccagttc
     8701 ctataattgg tgcttctctt ctaactagag gcactgcctg acgctgcatg ttcgctccca
     8761 taagggcacg gttggcatca tcattttcta agaaaggtat catagcagtg gcaacagata
     8821 caacctgctt aggagatata tccatgtaat ctactctact ttcaggaacc atttcaacag
     8881 caccatttac agttctacaa acaactctat ggtttacgaa tttaccatct tctgttaatg
     8941 gctcatttgc ctgagctctt acgaataaat cttcttcgtc agcagttaaa taatgtattt
     9001 catcagtaac tgttgatgtt tctttatcaa attttctata tggtgattct ataaatccaa
     9061 attcatttat ttttgcataa gttcctagag agtttataag accgatattt ggtccctctg
     9121 gagtctctat tggacacatt ctaccatagt gtgaatggtg aacgtcacgc acttcgaatc
     9181 cagctctttc tcttgaaaga cctccaggcc caagggctga taatcttctc ttatgcgtta
     9241 actcagatag aggatttgtt tgatccatga actgagataa ctgagaacta ccaaaaaatt
     9301 cttttattgc agctgaaact ggtcttatgt taactaacgc ttgaggcgtt atagcttcca
     9361 tatcttgaac cgtcattctt tcttttataa ctctttccat tcttgaaaga ccaattctaa
     9421 cttgattttg taacaattca cctacagatc ttattcttct gtttcctagg tgatctatat
     9481 catcaatatt tcctatatta tagaatatat tgaactcata acttattgaa gctattatat
     9541 catctaataa tatatgtttt ggtataagtt ctttaattct tgattttata gcctctttta
     9601 tttcttcttc atcactatat ccatctaaaa tttcttttaa agttggataa tgtacttttt
     9661 cttttatgtt taaatcatct atgtcaaaat caatgtgaga cttaatatcc acaaaattat
     9721 ttcctataac tctaactttt ttatcatcat ccattagtag atttactaca tttatacctg
     9781 cattttgtat ggctttagcc aaatcatagc ttattttttc tccagctttt acaaatactt
     9841 ctccagtttc tggatttatt atgtcttctg ctgaaatttt attcattatt ctgtaacata
     9901 aagcaagttt cttattgaat ttatatctac caacttttgc taggtcatat ctttttgggt
     9961 caaagaataa agcatttaat aaagatgatg cactttctac agtaggaggt tcacctggtc
    10021 ttaatttttt gtatatctct acaagacctt cttctactgt cttagtatta tctttttcta
    10081 atgtagcaga taatctctca tcttctccta aaaggtcaat tatctctgca tctgttccta
    10141 ttcctaaagc tctaagtaaa actgtaactg gttgttttct tgttctatca actcttacag
    10201 atattacatc attagaatct gtttcatatt ctaaccatgc tcctctatta ggaattacag
    10261 tagatgaaat caatctctta cctgttttat ctctttcttc cgcatagtaa acaccaggag
    10321 atctaactaa ttgacttact ataactcttt ctgctccatt tataacgaaa gttcctcttt
    10381 ctgtcattaa agggaagtca cccataaaca cttcttgttc ttttatctca cctgtttctt
    10441 tatttataag tcttacctta acctttagag gtgcacaata tgtagcatct ctctctttac
    10501 actcttctat atcgtacttt ggtttatcat ctagagaata gtcgacaaat tccaatataa
    10561 gattaccagt ataatcttct attggtgaaa tatcatcaaa cacttctttt aaaccttctt
    10621 ttaaaaacca ctcataggaa tctacttgaa tttctataag atttggcaca tcagctattt
    10681 ccttaatttt agaaaagctc attctagttc ttttacctat cgtgacagga tgtggcattc
    10741 acttttcacc tctcaataat taaaaataaa acaatagttc attctaacgc atctaataat
    10801 tttagcataa tgtcaagtat ttttcaatat attttttatc ttatatgtaa actatgcatt
    10861 atttttgatt ttttgtaaat tattttaagc atatattatg tattatgtac aattttccaa
    10921 tataaaatcc atatttttca tttataggtc taattttcta taaaaaattt tatacaagta
    10981 ccatcccccc tatttaatta taattccata ttaaaatgat tgtaaataat tacatttata
    11041 caaatatata caatttatta aaatatacaa aataaagacc ataaataaat ttaacttgga
    11101 attttgaacc tcaaaccttt ccaatataaa ttatatctat ggcctttata aaaatatcat
    11161 ttctactata actaatgcat aagctttcat tgcatattag taatttaatt atcctaatat
    11221 attatctgca aattgtggag taggtaaacc aaatcctcct gcaacttcaa gtaaatcccc
    11281 tgttataaat gatgagtcat cactagcata gaatagaaca gcttttgcta tatcatctgg
    11341 ttcccctata cgatttaaag gaacatgctt taaaaattct tttatgaact ctggagacat
    11401 attgtctaat gctgctttag ttgcaataag ccctggaagt acagcattac atctaacatt
    11461 gtctttagca tattgtgtag ctatgttttg agttaatgag ttgattgctg cttttgatac
    11521 acagtaagct attctagata aatcaggtaa tactgaacct atacttgata tatttactat
    11581 acttcctttt ccattcttta tcatatgagg aattgctgct ttacatggta aatacacact
    11641 ttttaaatta gtatttactg tgtcaaagaa attatctgta tctccagtta caaggtcttt
    11701 gtcaagagaa gggtttgttg aaccaaaatt gtttacaagt atatctatct taccttcttt
    11761 tttaactacc tcttctatca ttgaggtaaa ggtctcttct tcacgagcat taaagtaaac
    11821 aaacttagca caaccacctt ctgcacttat tttatttata acttcattag ctaattcttc
    11881 tgaacgagct gctaagtaca cagttgctcc attttttgct aatatctctg ctgatgctaa
    11941 tccaatacct tttgttgctg cagtaactac tgcaattttt ccttgtaatt tttccattat
    12001 taactcctcc ttgaatatgt cctttaaatc ctacaaattt cgatttttct aaatatctaa
    12061 cttattttaa aatttatatt tgttaattaa ttatttatta cattttattt ataaaacact
    12121 cgtgtatttt tttaaactaa aatgtaaaat tttacatttt agtttaatta tatactttat
    12181 atacagacat tacaacaatt gtttatattt taatttgata ataatgttaa attaaaatac
    12241 cattacacca tatatgatac ccttaattta gtactattat ataaattttt ttattttata
    12301 aaaatacttt ctttataatt ccaaatactt tctttatagt tttttataaa taaaaaaaga
    12361 tacccaatgg gtatcttttt aaagaaacta cttaacttct actttagctc cagcagcttc
    12421 taatttctct tttatttgat cagcttcttc tttagaagct ccttctttaa ctgtcttagg
    12481 agcattgtca actacttctt tagcttcttt taatcctaat cctgttattt ctctaactgc
    12541 ttttataact ccaacttttg aagaaccaac gtcagttaat actacatcaa actcagtttt
    12601 ttcttcagca gctggtccac cagcagctgc accagctacc attactggag ctgaagctga
    12661 tacaccgaat ttttcttcag cagctttaac taattcattt aattctaaaa ctttcatatt
    12721 ttctatagct tctaatattt gttctattgt catttttagc acctccgaat tttatttcaa
    12781 attaatattt ataatttttt agttaagcaa ttaagcttct tcttgacctt ctgccttttt
    12841 cgctattgca tctattaagt atgcgaagtt tgatactgga gcttttaagc taccaagtaa
    12901 tttagcgata agaacttctc ttgatggtat gttagccatt tcaacaatct tagattcatc
    12961 atagaatgca ccttccacaa tacccatctt taatttcatt tttggatgag aatccatgaa
    13021 tccttttaat attctagctg gagcaactgg atcatcatat ccaaatgcta tggcattagt
    13081 tccaactaat aattcatcat taaattgctc tataccaact tctttagctg ctcttctaac
    13141 taaagtattt ttgtatactt tgtaatctac accagcttct ctcatttgtt ttcttaactc
    13201 agttacttct tcaactgtta atcctttgta atctacaaca acagcagcag aagatttttg
    13261 taacttttca actatctcag aaacaacttc tgatttaatt tctatagctt ttctcatata
    13321 gatgtgcacc tccttcacca aataggctta gtatttaccg caacattata aaaagctttt
    13381 gttgccaaaa gtagacatca aaagctttag taatctcaat ttatctaaaa cctcggcggg
    13441 atattaagct ttcgcaccca ctatctacgg caaatctatt cttttttaca ttgacaagtg
    13501 atataataac atttttgtca acctgtgtca acagttttat tattcagctg ttcttgctgg
    13561 gtttatttta actccaggtc ccattgaaga agctactgtt atacttctta aatattgtcc
    13621 tttagcagca gctggtttag cttttattat agcatccatt aatgcagtaa agttttcagt
    13681 taatttttct ccaccaaatg atacttttcc tactggaacg tgtattatgt tagttttgtc
    13741 taatctgtat tcaactttac cagcttttat ttcatctata gctttagcta catcaaatgt
    13801 aactgttcct gatttagggt ttggcattaa acctttaggt cctaatactc tacctaatct
    13861 acctactaca cccatcatat ctggagtagc aactactata tcaaagtcaa accagttttc
    13921 accttgtatt ttttgaacta attcttcagc tcctacaaag tcagcaccag cttgttcagc
    13981 ttctttagct ttttcacctt tagcaaaaac taaaactctt tttgttttac cagttccatg
    14041 aggtaataca actgcacctc ttacttgttg gtcagcatgt cttgagtcaa cacctaattt
    14101 aatgtgtgct tctactgtct catcaaattt agctccagct atatctgaaa ctaatgttaa
    14161 tgcttctgat gcatcataaa atttagttct atctactttt tctaatgcac ctgcatatct
    14221 tttacctttt ttagccattt tattttcctc ctcgtggtta atttcggctt ctagcctccc
    14281 actttataaa aaaaagtgta tcttttaatt agtcttcgat tacaacaccc atgcttctag
    14341 cagtaccagc tatcatactc atagcagctt ctactgatgc agcatttaag tcaggcatct
    14401 ttaattcagc tatttctctt acttgagctg aagtcatttt agcaactttt ttcttgtttg
    14461 gttctccaga acctgatttt aagtttaatg cttttttaat taaaactgca gctggtggag
    14521 tttttgttat aaaactgaat gatctatctt gatatacagt tataacaact ggtattatca
    14581 ttccagcttg atctgcagtt ttagcattaa attcttttgt gaatcccatt atattaacac
    14641 catgttgtcc taatgctggt ccaactggtg gcgctggagt agcctttcct gcaggtattt
    14701 gtaattttat ttgacctata acttttttag ccattttgag cgcacctcct cagatttttc
    14761 tataatatag tattagattt tttctatacc ttcaagctct attacaaata gagtcctttt
    14821 accaaatgca tttatgcata ctttgacttc tctattttct aagtcaattt cttctatgtt
    14881 acctatttgt ccactaaatg gtccttgaga tacttttact gtatctccta tttcaaaatc
    14941 aatatcgcta tttactactt taggatctgt tgtgtctatc cccatagctt tgacttcgtc
    15001 ttcacttaat ggcactggtt ttgaaccagg tccaacgaaa cctgtaactc ccttagtatt
    15061 tcttactaca taccaagatt catcagttat tatcatttta accaaaacat aacttggata
    15121 aactttacgt tgtctagttt tttcttttcc agttttagta gtttccacta cttcttctgt
    15181 tggaacaact acttgtctta tgcagtcttc catacctctt gttttaactg ctttctcaat
    15241 tgtagcttta actttatttt catgtcccga ataggtatga actacatacc aacttgcttc
    15301 ttgtaattct gacatttaac ttacctcttt tcgtctattt tagtaataac gctagtgcac
    15361 cagataatat agtgtctata ccccatacta atatagtgca tgaaataact acagttaaaa
    15421 ctatacttgt gtttttaaac agttcctttt ttgtcggcca tgtaactctt tttaattctt
    15481 gtttagtttc ttttaagtat ccaaacaaac taaacctttt tttagttttt gcaccgtcat
    15541 ttttttgggc agccattcca tactcacatc cttataaaaa actattttgt ttctttatga
    15601 gttgtatgct ttttacagaa tctacaatat ttttgtaatt ctattctgtc tggattgttt
    15661 ttcttattct tagtagtgtt gtagtttctt tgcttacact ctgtacatgc taaagttact
    15721 ttaactctca tctgatacac ctccaacttt gtaatacaat aatatatatt gttaatatat
    15781 gtgaatataa tatctttaaa atatgtattt attaaaaatc tacagtattc aagatattat
    15841 taacttccta taatcattac cttgtataag ttatcacaaa atcctatgct gtgtcaatgt
    15901 ttttgtattt ctatgttaaa aaaagaggag cataacccct ctttttgtta ttccttttaa
    15961 tccatcagtt tagtaaag
//
LOCUS       DBGJFF010000087        15820 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.088, whole genome shotgun sequence.
ACCESSION   DBGJFF010000087 DBGJFF010000000
VERSION     DBGJFF010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15820
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.088"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(<1..1079)
                     /locus_tag="ACOIAF_002667"
     CDS             complement(<1..1079)
                     /locus_tag="ACOIAF_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HGZ2669671.1"
                     /translation="MKKILKRLCTGVLAFATVVTALPSTAVHAESKQYWTESEERVGI
                     VEKVMNDGSIGSTFNEGHLTVEGEDAYCIDINTDFKNGYKTRADASTRMSADQISDVA
                     LSLEYVKQYGEAHKELNYKQVYLLEQCVVWQRLSVHLGWQCDNVRASYDEIPKATQDE
                     VFAGAKAFVKENKGRYECGGYIYSGEGQELGQFWAKLNVGNAKLQKTSSNTSITDGNG
                     NYSIAGATYGVFSDKDCTKQLATLTTDKNGNTDVVEVKAGTVYIRELSAPAGYKVDTT
                     VYSLKVEAGKTATLNVSDTPKVTDTLIELFKIDMETQKDNPQGNASLEGAEFTWKYYA
                     GFYNKDNLPAEATRTWVTKTIAETD"
     gene            complement(1322..2686)
                     /gene="rlmD"
                     /locus_tag="ACOIAF_002668"
     CDS             complement(1322..2686)
                     /gene="rlmD"
                     /locus_tag="ACOIAF_002668"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HGZ2669672.1"
                     /translation="MLSKNEMYIVDIVDIGQGGVGVGKYNGITVFVEGGLIQDKVKVR
                     INKVKKNYAVGDIVEIIEKSPFRVKRVCSDNLRDCGGCQIQDLDYNKQLEMKTNEVKQ
                     VLSRIGKLEDVEVHDTIGMDNPYRYRNKAQFPIQKNNGIPIIGFYKKKTHDVISTEKC
                     VIQHEINDKIVKIIKTYIRAYKVSIYDEKTHTGLIRHLVTKIGFTTKEVMVVLVANGN
                     KLPYLKELASVLKENIPGFKTLVVNVNQKDTNAILGRENIVIYGDGKINDYIGDLVFE
                     ISPLSFFQVNPVQTKVLYDKALEYANLNDTDTVFDIYCGIGTISLFLAQKAKKVYGIE
                     IIDDAIKDAKINAKLNNIHNAEFYVGKAEEIVPKMYREGKTANVVVVDPPRKGCDEKV
                     LETIVSMNPERIVYVSCNPSTLARDLDYLNVHGFKCVEAQPVDMFPHSTHIETCVLLQ
                     RRTM"
     gene            complement(2861..4621)
                     /gene="pyk"
                     /locus_tag="ACOIAF_002669"
     CDS             complement(2861..4621)
                     /gene="pyk"
                     /locus_tag="ACOIAF_002669"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HGZ2669673.1"
                     /translation="MLNNVKKTKIVCTLGPASQSEEVLTQLMQNGLNVCRFNFSHGSH
                     EEHKERIDMAKKVREKLNKPVAILLDTKGPEIRTGNFEDPEVFLEEGQKFTITMKDIM
                     GTKEMCTVSYKGLAEDVKSGDSILIDDGLVGLRVKEINGEDIVCVVENSGIVKNHKGV
                     NVPGVKINLPAITPKDISDIEFGISQGIDYIAASFVRKASDVLAIREVLENNNATDIQ
                     IISKIENQEGVENLDEILKVSDGIMVARGDLGVEIPTEEMPIVQKMMIKKCNELAKPV
                     VTATQMLDSMIRNPRPTRAEVTDVANAIYDGTDAIMLSGETAAGKYPVEAVKMMATIA
                     KRTEETLDYDRLLKENGTNNVTVTDAISHATCTTAVDLNASAIITSTSSGYTARMVSK
                     FRPKSPIIATTNNEKTMNKLALTWGVYPVKSSVAGNTDEVIEKAIEAARQADYIDNGE
                     LVVITAGVPVGVSGTTNLIKVHVISEEIVQGIGVGTQTVEGKVRIIKNGNACVDFNEG
                     DILVSTATDAEMNNYIEKAGAVVTENGGMTSHTAIVGINLDIPVIVSATDITNLVKDG
                     EVVTVDASRGVVYRGSTRVL"
     gene            complement(4673..5632)
                     /gene="pfkA"
                     /locus_tag="ACOIAF_002670"
     CDS             complement(4673..5632)
                     /gene="pfkA"
                     /locus_tag="ACOIAF_002670"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HGZ2669674.1"
                     /translation="MKTIGLLTSGGDAPGMNAAIRAVVRSAIYYGCKVYGINRGYKGL
                     LEEDLTEMNLSSVGDIIHRGGTILKSSRCEEFKTEEGRLKAVKILKKYKIDCLVVIGG
                     DGSFAGAQKLSDLGFPAIGIPGTIDNDLAYTDYTIGFDTAMNTIIDAIGKIRDTSSSH
                     ERVNIVEVMGRHCGDLALYAGLAGGAETIIVPEVEITVDEVALRLKTTQKRGKRHSII
                     VLAEGVGSASDLEKELKKESGADLRVTVLGHVQRGGSPTVSDRILASRLGVRAVELLL
                     DGKSARVVGIKENKIIDLEISEALAQKKVFDKEAYEMAKILSI"
     gene            complement(5841..9410)
                     /locus_tag="ACOIAF_002671"
     CDS             complement(5841..9410)
                     /locus_tag="ACOIAF_002671"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HGZ2669675.1"
                     /translation="MNKDFAHLHVHTEYSLLDGFSRVKKLIKRAKELNMSSIAITDHG
                     CMFGVIDFYKTAIKEGIKPIIGCEVYTAARGLRDKDPNYDKYQGHLVLLAKNMEGYKN
                     LIKIVSTSYVEGFYYKPRVDMEELKKHNKGIIALSACLAGDVARALMNRNYEKAKQFA
                     LDYRDIFGEENFFLEIQDHNLPEQKEVNSGLVKLSKETGIPLVATNDVHYVNKEDSKI
                     HDVLMCIQMGKTLNDPNRMRFGSDEFYLKSREEMEELFPYALEAIDNTVKIADMCNVE
                     FDFNTIHLPKYDVPEGYTPETYLRELCFNGLKERYDNPSNEILERLNYELDVIEKMGY
                     VEYFLIVWDFINFSKENNIIVGPGRGSAAGSIVAYTLKITDIDPIKYSLLFERFLNPE
                     RISMPDIDIDFCYERREEVIDYVKRKYGDDHVAQIITFGTMGAKAAIRDVGRVLDIGY
                     NKVDKVAKEIPFALGMTIDKALDTNPNLKELYDQDPETKEIINISKQIEGMLRHASTH
                     AAGVVISKNPVDEYVPLYKHQDAITTQFTMTTLEELGLLKMDFLGLRTLTVIRDTLDL
                     IEKNRVIKNYTEKIDFSKMDYDDPKVYEMLSSGNTLGVFQLESAGMRSFMKQLKPDNF
                     EDIVAGISLFRPGPMDSIPAYIKNKSNPKDVTYLHEKLKPIMEVTYGCLVYQEQVMQV
                     VRDLGGYSYGRSDLVRRAMSKKKMDVMEEERQYFIHGKFDEDGNIEIAGCIRNGVEEE
                     IANKIFDDMIDFARYAFNKSHAAAYGVLAYETAYLKTYYPVEFMAALITSVMGNTDKV
                     VEYIRECKALEIDVLPPDINKSFSKFSVEGDNIRFGLAAVKNVGVNIINNIIAERESN
                     GLFVDLVDLVKRLDQKDTNKRVIESLIKCGAFDNISENRASLMAGYESLLESVSMDRK
                     KNVQGQISLFDAFGDSEEDSDFQQIYSLPKRQEYEERERLNLEKEVLGMYVSGHPLSQ
                     FEKELQEKTSMDNGKLNSLKEEEESYLQMNETDVIMGGMIVTKTIKTTKRNEIMAFIE
                     LEDLYGAIEIIVFPQLLQKYNVILNEDNIIYVKGTLSIKEGENAKLIAREIKDINDDT
                     DFADRNYNSGRTRNVENIKPESGKKLYLKIDSMSDTDTTNNIVEIAKQYPGNDSIYLY
                     PMDENINGKRKAYKMTGALTCICDEFINNLEELLPKDNIKVKA"
     gene            complement(9567..10514)
                     /gene="whiA"
                     /locus_tag="ACOIAF_002672"
     CDS             complement(9567..10514)
                     /gene="whiA"
                     /locus_tag="ACOIAF_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HGZ2669676.1"
                     /translation="MSFSTETKNELARVISGNECCNIAELSALVKSGGSIQIVGYKKL
                     NLKITTELNSIARKVFKLLKKNFNINTTISVNKNQMLKRNNSYVLMVTSEMGSEMLLK
                     KLGILEDKEGFFTINKISENLIKHEGCKRAFIRGAFLGGGSISDPEKNYHMEFVTNNE
                     EFADSLKELINSLGFNSKIVARKNNHVVYIKESEQISDLLSIIGGHHALLSLQNTKIV
                     KEMRNNVNRIVNCETANLSKTVNAAVRQVENIKLIQETIGISSLPENLQEIAKIRIEY
                     EDMTLKELGEMLEPPIGKSGVNHRLRKIEEIATDLKKKS"
     gene            complement(10531..10956)
                     /locus_tag="ACOIAF_002673"
     CDS             complement(10531..10956)
                     /locus_tag="ACOIAF_002673"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HGZ2669677.1"
                     /translation="MREEVSAGGVVLFGNTILLLRKFNGDWVLPKGKVEEGENNQEAA
                     LREVSEETGVKADILKYLGEIHYTFKENWDENRAVHKTVFWYLMQAKNMDTIPQKEEG
                     FIDAKFIHLDRVVDLARYDDEKEIIKVALQEIKKRLKKN"
     gene            complement(11025..12131)
                     /gene="yvcK"
                     /locus_tag="ACOIAF_002674"
     CDS             complement(11025..12131)
                     /gene="yvcK"
                     /locus_tag="ACOIAF_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HGZ2669678.1"
                     /translation="MAKLLIIVGILGWIALIVSLFIYKKAKNEKVKLFQRSIVENREP
                     NVVVIGGGTGQSVFLRGLKHTTQNITAIVTVADDGGGSGVLREDLGMLPPGDIRNCLL
                     ALANIEPTMNEVMQYRFTEGLLKGQSFGNLFLAAMNGLYGNFEKAVYKLSEIFAITGR
                     VLPVTLEDVNLVAKLENGNIINGESSIPEESKIQKSSIDKIFLNPKDVKPLKDVIASI
                     YDADIIIMGPGSLYTSIIPNLLVEGIVDAIKSSVAPKVYIANIMTQPGETEGYNVLEH
                     VNAIVKHTDENLIDYVIANNEILPEEMLDLYKQDGAEQVLLDKKQKDKLKEIGIKTVE
                     KNLIEIKNNYIRHDAKYISNIVIELALNHNYNKS"
     gene            complement(12143..13000)
                     /gene="rapZ"
                     /locus_tag="ACOIAF_002675"
     CDS             complement(12143..13000)
                     /gene="rapZ"
                     /locus_tag="ACOIAF_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HGZ2669679.1"
                     /translation="MKFVIVTGLSGSGKSETMRALEDMGFYCVDNLPPALITKFAELC
                     YQPNSSIDKVALGIDIRGRKFFEALHESLNYLEKENYEYEMVYLDCNDDVLLKRYKMT
                     RRNHPLAKDMQIPEGIKMERKIMEPLKELSTCIIDTTNMKPKDLKEEIKKIYSSGEDN
                     PNLTISVVSFGFKHGILADADLVFDVRFLPNPYYVEELRAKTGDDKEVRDYVMNSKIS
                     EEFYVKLLDMIHFLVPQYIEEGKQHLVIGVGCTGGRHRSVTITNLIAEDLSNKGYRVV
                     KKHRDSMLR"
     gene            complement(13031..13744)
                     /locus_tag="ACOIAF_002676"
     CDS             complement(13031..13744)
                     /locus_tag="ACOIAF_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HGZ2669680.1"
                     /translation="MKILADYHTHTLYSHGKGTIEDNVKVAISKGIKTIGISDHSYKH
                     IAYGVKKKDILKMREEIDYLNDKYNDINILLGMECNILDDKGNIDMDDKFIRDFDYIM
                     AGYHFGSTPTSLRSLLNHCNNYIFKTEKAKEYNTKAVINAISNNDIFIITHPGDKGDV
                     YIEEVAKTAKKTNTRLEINSSHSFLNVNQLNKIKGLENKFIIGSDAHCPERVGDFELA
                     MKTISDSNLDISLVENIII"
     gene            complement(13748..14662)
                     /gene="murB"
                     /locus_tag="ACOIAF_002677"
     CDS             complement(13748..14662)
                     /gene="murB"
                     /locus_tag="ACOIAF_002677"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HGZ2669681.1"
                     /translation="MNNQDIYINLLNILSKEDIKVDEPMKKHISFRVGGPADILVRPR
                     TEEQLKNVLKLVKEESIPYLIIGNGSNILIKDGGIRGVVIELADNFNSYEINDTRMTA
                     QSGALLSVLGKALQKQELKGFEFASGIPGTLGGALAMNAGAYGGEMKDIVKSVRLMDM
                     EGNIFELSNEQMEFGYRKSIISKNGYIALSAELELQEGNYDEIKSLMDDLATKRITKQ
                     PLNFASAGSTFKRPTGYFAGKLIEETGLRGLTLRGAQVSEKHCGFVVNQGEASAKDIL
                     DLIYVIKSAVYAKFGVMLEEEVKILGED"
     gene            complement(14935..15705)
                     /locus_tag="ACOIAF_002678"
     CDS             complement(14935..15705)
                     /locus_tag="ACOIAF_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HGZ2669682.1"
                     /translation="MSKGFLTPGETAQATISAGIKKANISTQNAILLGLFGGAFIGFG
                     ALGSMIVSQTFGKIDPGVASFLSAMVFPVGLMLVVVAGAELFTGNTLMAIALMDKKIT
                     LGKMLRNWGLVYFANLVGSILLVALVFYSSTLTGDAATKAIAVAEAKVNSTIASMFLK
                     AILCNILVVLAVWMATASQDIMSKLASCWTVIMLFVLCGFQHSVANMFFIPMGMALGA
                     NITMVQFITNLVFVTLGNIVGGSIVVGGIYYLCYVKNS"
BASE COUNT         4638 a         3202 c         1784 g         6196 t
ORIGIN      
        1 ctgtctgttt cagcgattgt ctttgtaacc catgtacgag tagcttcggc agggagattg
       61 tctttgttat agaagccagc ataatacttc catgtaaact ctgctccctc taaagaagcg
      121 tttccctgcg gattgtcttt ctgcgtttcc atgtcaatct tgaaaagctc aatcaaggtg
      181 tccgttactt ttggtgtatc tgatacgttc aaggtcgctg tctttccagc ttcaactttt
      241 aaggaataca cggttgtatc aactttatat cctgctggtg cggataattc cctaatatag
      301 actgtacctg cttttacctc tacaacatct gtatttccgt ttttatcagt cgtaagggtg
      361 gcaagctgtt tcgtgcagtc cttatcagaa aagacaccat aggtcgcacc agcgattgag
      421 taattcccgt taccgtctgt aatgctggta ttactggaag tcttttgcag ttttgcattt
      481 ccgacattta acttcgccca gaattgtccc aattcctgcc cctcgccaga gtagatataa
      541 ccgccacatt catagcgtcc tttattttct ttcacaaagg ctttagcacc agcgaaaact
      601 tcgtcctgcg ttgcttttgg tatttcatca taagaagctc gcacgttgtc gcattgccag
      661 ccaagatgta cgctcaatct ctgccagaca acacattgtt ccaacagata gacttgcttg
      721 tagttcaatt ccttgtgtgc ttcgccatac tgtttgacat actctaagga taacgccaca
      781 tctgaaatct ggtcggcact catacgggtg cttgcgtcag ctctggtctt gtagccattc
      841 ttaaaatctg tattgatgtc tatacagtaa gcgtcctcgc cctcaacagt caaatgtccc
      901 tcgttaaagg tagaaccaat cgaaccgtca ttcattactt tttctacgat accgacacgt
      961 tcctctgatt ccgtccagta ttgcttgctt tctgcatgga cggcggtact cggtaaagca
     1021 gtaacgacag tcgcaaaggc aagtactccc gtacacaatc gtttcaatat ctttttcata
     1081 attctaaatg ctcctttcat tttgggtaaa aaaatagacg ctcaatattg aacgtcttac
     1141 attgaaaagg gatacttttt acaagtaccc ctcaaatcta atatttaatt gtttgtgttc
     1201 ccacactcta tatcaacaac tactgaaaca ttgatatagt tttttatctc tttatttctg
     1261 attttatcgc cctcgctcgt aaagactata tgcttaaaaa agtgcctaaa attaaggatt
     1321 tctacatagt tcgtctttgt agcaacacac aagtctctat atgtgtagaa tgagggaaca
     1381 tatctacagg ttgtgcctca acacatttaa atccgtgaac atttagataa tctaaatccc
     1441 ttgctaaagt agaaggatta caagaaacat agacaattct ttccggattc attgaaacaa
     1501 tagtctctaa taccttttcg tcacatccct ttctaggtgg gtctacaacg actacattgg
     1561 cagtcttacc ctctctatac atcttaggta caatttcctc tgcctttcca acataaaatt
     1621 cagcattatg tatattgttt aatttagcat ttatcttagc atcttttatg gcatcatcta
     1681 taatttctat tccataaacc tttttagcct tttgtgccaa aaataaagat attgtaccta
     1741 tcccacagta aatatcaaaa acagtatcag tatcatttaa atttgcatat tcaagagcct
     1801 tatcatacaa tactttagtt tggacagggt ttacttggaa aaatgataat ggtgaaatct
     1861 caaacactaa gtctcctata taatcgttta tttttccatc tccatatata actatatttt
     1921 ctcttcctag aatagcattc gtatcttttt gatttacatt tacaaccaaa gtcttaaatc
     1981 caggtatatt ttccttaagc actgaagcta attctttaag atatggaagt ttgtttccat
     2041 ttgctactaa tacaaccatt acttctttag ttgtaaaacc tatttttgtt actaaatgcc
     2101 taatcaaccc cgtgtgagtc ttttcatcat atatacttac cttataggcc cttatatatg
     2161 tttttattat tttaacaatt ttatcattta tctcatgttg tattacgcat ttttctgtcg
     2221 atattacatc atgagtcttc tttttataga agccaataat tggaatacca ttattctttt
     2281 gtattggaaa ttgtgcttta tttctatatc tatatggatt atccattcct atagtgtcat
     2341 gaacttctac atcctctaac ttacctattc tcgaaagtac ttgtttaact tcatttgttt
     2401 tcatttcaag ttgtttatta taatctaaat cttgtatctg acaccctcca caatccctaa
     2461 gattgtcact acacactctt ttcaccctga atggagactt ttctattatc tcaacaatat
     2521 ctcctacagc ataatttttc tttactttat taatcctaac tttaacttta tcctgtatta
     2581 atccgccctc tacaaaaact gtaataccat tgtactttcc tactcctact ccgccttgac
     2641 caatatctac tatatctaca atatacatct cgtttttcga aagcataaat caccctccaa
     2701 tcaatctatt atatttaaat ctttacaaac ttttttcttt aaatttatat taaaatattg
     2761 ctattaatta aactatattt tttactaagc tacaatattt tattttacca aaaaaaaagg
     2821 agcttataaa gccccttagt aaataaattt attatttttc ttataaaact ctagttgaac
     2881 ctctatatac cacgcctctt gatgcatcca cagttacaac ttctccatct tttactagat
     2941 ttgttatatc agttgcagac acaataactg gtatatctaa atttatacct acaatagctg
     3001 tatgtgatgt cattccacca ttctcagtta ctacagctcc tgctttttct atataattgt
     3061 tcatttcagc atcagtagca gtagaaacaa gtatgtcgcc ttcattaaag tctacacaag
     3121 catttccatt ctttattata cgaactttac cttctaccgt ttgagtacct acacctatac
     3181 cttgcacgat ttcttcgctt attacatgaa cctttattaa attagtagtt ccacttactc
     3241 ctactggtac tccagcagtt ataactacta attccccatt atctatataa tcagcttgtc
     3301 tagctgcctc aattgccttt tctataactt catctgtatt tcctgcaaca gaagacttaa
     3361 ctggatatac tccccatgta agagctaatt tattcatagt cttttcatta tttgtagtag
     3421 ctattattgg agattttggt ctgaatttag ataccattct agcagtatat cctgaagaag
     3481 tagatgttat tatagctgat gcatttaaat ctactgctgt tgtacaagtt gcatggctta
     3541 ttgcatctgt tacagtaaca ttgttagtac cattttcctt taataatcta tcataatcta
     3601 aagtctcttc tgttcttttt gctattgtag ccatcatttt tactgcctct acaggatatt
     3661 ttccagcagc agtttctcca gataacatta tcgcatctgt tccatcatat atagcattgg
     3721 caacgtctgt aacttctgct cttgttggtc ttggatttct tatcatagaa tcaagcattt
     3781 gagtggcagt aacaactggc tttgctaatt cattacattt ttttatcatc attttttgaa
     3841 ctattggcat ctcttcagtt ggtatctcaa cacctaagtc acctctagcc accattatac
     3901 catcagaaac ttttaatatt tcatctaggt tctctacacc ttcttggttt tctattttag
     3961 atattatttg tatatcagtt gcattattgt tctctaatac ttctcttatt gctagtacat
     4021 cagatgcctt tcttacaaaa gaagcagcta tataatctat tccttgactt attccaaact
     4081 ctatatcact tatatcttta ggagttattg ctggtaaatt tatcttaaca ccaggtacat
     4141 ttactccttt atgattttta actattcctg aattttcaac aacacaaact atatcttctc
     4201 catttatttc ttttactctt aagccaacta aaccatcatc tattagtata gaatctcctg
     4261 atttaacgtc ttcagcaaga cctttatagc ttactgtaca catttcttta gtccccataa
     4321 tatctttcat agttatagtg aacttttgac cttcttctaa aaatacctct gggtcttcaa
     4381 agttacctgt tctaatttct ggacctttag tatctaataa tatagcaact ggcttattta
     4441 atttttctct aactttcttt gccatatcaa ttctttcttt atgttcttca tgcgaaccgt
     4501 gagaaaagtt aaatctacac acatttagac cattttgcat aagttgagtt aaaacttcct
     4561 cactttgtga tgctggacct agtgtacaaa caatttttgt cttctttaca ttatttaaca
     4621 tatttgctcc tcctgagata ttattccaca gatttatttt taatactaaa acttatatag
     4681 ataatatttt tgccatctca taagcttctt tatcaaatac tttcttttga gctaaagctt
     4741 cacttatttc taaatcaatt attttgtttt cttttattcc tacaactcta gctgattttc
     4801 catctaataa taattctact gctcttactc caagtctact tgctagtatt ctatcagata
     4861 cagttgggct tcctcctctt tggacatgcc ccaatacagt gactcttaaa tctgctcctg
     4921 actctttttt aagttccttt tctaaatcac tagcacttcc gactccttct gctaaaacga
     4981 ttatactatg tctcttacct cttttttgag tagtctttaa tcttagagca acttcatcta
     5041 ctgtaatttc tacttctggt actattatag tttccgctcc tcctgcaaga cctgcatata
     5101 aggctaaatc tccacagtgt cttcccataa cttctactat gttaactctt tcatgagatg
     5161 atgatgtatc tcttatctta cctatagcat ctataatagt attcattgca gtatcaaatc
     5221 ctatagtata atctgtatat gctaaatcgt tatctatagt tcctggtatg cctatagctg
     5281 ggaatcctaa gtcactaagt ttttgggctc ctgcaaatga accatctcca ccaattacaa
     5341 ctaaacaatc aattttatac tttttaagaa tttttactgc ttttaatcta ccttcttctg
     5401 ttttaaattc ttcacatctg gaagatttta atatagttcc tcctctgtgt ataatatctc
     5461 caacagaaga taagttcatt tcagttaaat cttcttctag taatccctta tatcctctat
     5521 tgattccata tactttgcat ccataatata ttgctgatct tacaaccgct ctaatcgcag
     5581 cattcattcc tggagcatct cctccacttg ttagtaatcc tatagttttc attttttacc
     5641 tccttggatt ttatgtcaat tttttattat aatcattgtt attgtattct aatggacttt
     5701 ttttgtccct atatcacttt ttaatcccat caagactaaa aaaattaaat gtccttaaat
     5761 gtccattttg tgatattata tcacaaaacg gacaatattt ttcatattat tataatatat
     5821 ttagattttt atataaaaaa tcaagctttt acttttatgt tatctttagg aagtaactct
     5881 tctaagttat ttataaattc atcacatata caagtcaatg ccccggtcat tttatatgct
     5941 ttacgtttac catttatatt ttcatccata gggtacaagt atatactgtc attacctgga
     6001 tattgtttgg ctatttctac aatattatta gttgtatcag tatcagacat actatctatt
     6061 ttcaaataca attttttacc actttctggc ttaatatttt ctacatttct agttcttcct
     6121 gaattgtagt ttctatctgc aaagtcagta tcatcattta tatcttttat ctctctagct
     6181 ataagttttg cattttcgcc ttcttttata cttaatgttc cttttacata tattatatta
     6241 tcttcattca aaatgacatt atacttttgt aataattgag gaaaaactat tatttctata
     6301 gcaccataca aatcttctaa ttcaataaat gccataattt catttcgttt tgttgtttta
     6361 atagttttag ttacaatcat tccacccatt ataacatctg tttcattcat ttgtaggtat
     6421 gactcttctt cctcttttaa tgaatttaat tttccattat ccatagatgt tttttcttgt
     6481 agttcttttt caaattgtga taaaggatgt ccacttacat acattcccaa gacttctttt
     6541 tcaaggttaa gtctctctct ttcttcgtac tcttgccttt taggcaaact gtatatttgt
     6601 tgaaaatcac tatcttcctc actatctcca aaagcatcaa ataaagaaat ttgaccttga
     6661 acatttttct ttctatccat agatacactt tctaataaac tttcataacc agccatcaga
     6721 ctagctctat tttcacttat attatcaaaa gctccacatt ttattagact ttctattact
     6781 ctcttatttg tatctttttg gtctaatctt ttgactaaat ctactaaatc tacaaataat
     6841 ccattagatt ctctttcagc gattatattg tttattatat ttacgccaac attttttacg
     6901 gctgctagac caaatcttat attgtcgcct tcaactgaaa actttgaaaa acttttgttt
     6961 atatctggag gtaatacatc tatttctagt gctttacatt ctcttatata ctcaactacc
     7021 ttatcagtat ttcccattac actggttatc aatgctgcca taaattcaac tggataatat
     7081 gtttttagat aagctgtctc atatgctaaa accccatatg cagctgcatg tgatttatta
     7141 aatgcatatc tagcgaagtc tatcatatca tcaaatattt tattagctat ttcctcttct
     7201 acaccatttc tgatacaccc tgcaatttct atattaccat cttcatcaaa tttaccatga
     7261 ataaaatatt gtctttcttc ttccatcacg tccatctttt tcttactcat ggctcttcta
     7321 actaggtcac ttcgtccgta actataacca cctaaatctc taacgacctg cataacctgc
     7381 tcttgatata ccaaacaacc atatgtaact tccattatag gctttaattt ttcatgtaga
     7441 tatgttacat cttttgggtt gcttttattt tttatatatg caggtataga atccattgga
     7501 cctggtctaa atagggatat tcccgctaca atatcttcaa aattgtctgg ttttagctgt
     7561 ttcataaaac ttctcatgcc agcactctcc aattgaaaca cccctagtgt atttcctgat
     7621 gaaagcattt catatacttt cgggtcgtca taatccatct ttgaaaagtc tattttttct
     7681 gtgtaatttt ttataactct atttttttct attaaatcaa gagtatctct tataactgta
     7741 agagttctta gcccaagaaa atccatcttt aaaagcccaa gttcttcaag tgttgtcata
     7801 gtaaactgtg ttgtgattgc atcttgatgt ttgtacaaag gtacatattc atctacggga
     7861 ttttttgata taacaactcc tgctgcatga gtagaagcat gtcttagcat tccctctatc
     7921 tgttttgata tatttattat ttcttttgtt tccgggtctt ggtcatacaa ttctttaaga
     7981 ttaggatttg tatctagggc cttatctatt gtcattccta aagcaaatgg tatttccttg
     8041 gctaccttgt caactttatt gtagcctata tccaataccc ttccaacatc tcttattgca
     8101 gcttttgcac ccattgttcc aaaagttatt atctgggcta catggtcatc accatatttt
     8161 ctctttacat aatctataac ttcttctctt ctctcataac aaaaatctat atctatatct
     8221 ggcatggata tacgttctgg atttaaaaaa cgctcaaata atagagagta ctttattggg
     8281 tctatatcag ttattttaag tgtatatgct actatcgaac cagccgcact acctctacct
     8341 ggtcctacta taatattatt ttctttagaa aaatttataa aatcccaaac aattaaaaaa
     8401 tactctacat aacccatttt ttcaattaca tctaattcat aatttaatct ctctagtatc
     8461 tcattgctag gattatcata tctctctttt aatccattaa aacacaactc ccttaaataa
     8521 gtttctggtg tatatccttc tggaacatca tacttaggaa gatgtatagt attaaaatca
     8581 aactccacat tacacatatc agctattttc acagtattgt caatagcctc taatgcatat
     8641 ggaaataatt cttccatttc ttctcttgat tttaaataaa actcatcact accaaatctc
     8701 attctatttg ggtcatttaa tgtttttccc atttgaatac acataagaac atcatgtatt
     8761 ttcgaatctt ctttatttac atagtgaaca tcattagtag caactagtgg tataccagtc
     8821 tcttttgata attttactaa accagagttt acttcttttt gctcaggtaa attgtgatct
     8881 tgaatctcca aaaagaaatt ttcttctcca aatatgtctc tatagtcaag agcaaattgt
     8941 tttgcttttt cataatttct attcattaaa gcacgtgcta catctcctgc aaggcatgca
     9001 gaaagtgcta taatcccttt gttatgcttt ttaagttctt ccatgtcaac tctaggctta
     9061 tagtaaaaac cttctacgta tgaagttgaa actattttta ttaaattttt atatccttcc
     9121 atattcttag ctaacagaac taaatgtcct tgatatttgt catagtttgg gtctttatct
     9181 ctcaatccac gtgcagctgt atagacttcg caccctataa ttggctttat tccttctttt
     9241 atagctgttt tgtagaaatc tataacacca aacatacaac catggtcagt tatagctatt
     9301 gagctcatgt ttagttcctt agcccttttt ataagttttt tcaccctcga aaacccatca
     9361 agtaaactat actcagtatg tacatgaaga tgagcaaaat ctttgttcat taaatcacct
     9421 cctgctttat aattttaatt ttaccacaaa tatatgtact aaaacaaaaa cagagacttt
     9481 tatctcaaaa taggaattaa cttcaacgta taccattata tgttataaat taatgcttat
     9541 tctgagattg tcccctcaat ttattgctat gatttttttt tcaaatctgt tgcaatttct
     9601 tctatttttc taagtctatg attgactcca gattttccta ttggtggttc taacatctct
     9661 ccaagttctt tgagagtcat atcctcatac tcgattctaa tcttagctat ctcttgtaaa
     9721 ttttctggca aactgctaat tcctatagtc tcttgtatga gcttaatatt ttctacttgt
     9781 cttactgctg catttactgt tttagaaagg ttagctgtct cacaatttac tatcctgttt
     9841 acattatttc tcatttcttt tactatttta gtattttgaa gacttaaaag agcatggtgt
     9901 cctcctataa tactcaacaa atctgatatt tgttcactct ctttgatata aacaacgtga
     9961 ttattttttc tagccacaat tttactatta aaacctaaag agtttataag ttcttttaga
    10021 gaatctgcaa actcctcatt attagttaca aattccatat gatagttctt ttctgggtcg
    10081 cttatagaac ctccaccaag aaaagctcct cttatgaaag ctctcttaca tccttcatgc
    10141 ttaattaagt tctctgaaat tttgtttata gtaaaaaatc cttctttatc ttcaagtata
    10201 cctaactttt ttaataacat ttctgaaccc atttcgcttg ttaccattaa tacataactg
    10261 ttatttctct ttagcatttg attcttattt actgagattg tagtattgat attaaaattc
    10321 ttttttaata atttaaatac tttacgggct attgaattta actctgttgt tatctttaga
    10381 tttagcttct tatatccaac aatttgtata cttccccctg atttaactag agctgacaat
    10441 tcagctatat tacaacattc attcccagat ataactcttg ctagttcatt tttggtttca
    10501 gttgaaaaag acatatctat cacctattaa ctaatttttc tttaaccttt tttttatttc
    10561 ttgtaaggca acctttatta tctccttttc atcatcatac ctagctaagt ctactactct
    10621 atctaggtgg ataaatttag catcaataaa gccttcctct ttttgtggaa tagtatccat
    10681 attctttgct tgcatcaaat accaaaaaac tgttttatga actgctctat tttcatccca
    10741 gttttctttg aatgtatagt gtatttcacc taaatatttc aatatatctg cttttactcc
    10801 agtttcttca cttacttcac gtaatgctgc ctcttggtta ttttcccctt cttctacttt
    10861 tcccttgggt aacacccaat ctccattgaa cttccttagt aaaagaatcg tattgccaaa
    10921 caggactaca ccaccagcac taacctcttc tctcatcgat gttcactcct taaaactaac
    10981 ataatgataa ggatttctac acaatttttt aaaatccttt tttactaact tttgttgtaa
    11041 ttatgattta gggctaactc aataactatg ttagatatat actttgcatc atgtcttata
    11101 taattatttt ttatttcaat taaattcttc tctacagttt ttatacctat ctcttttaat
    11161 ttatcttttt gctttttatc taacaagact tgctctgctc catcttgttt atataagtca
    11221 agcatttctt ctggcaatat ctcattattt gcgattacat aatctattaa attctcatca
    11281 gtgtgtttta ctatagcatt aacatgctct aatacattat acccttctgt ctcacctggt
    11341 tgagtcatta tatttgctat atatactttt ggagctacag acgatttaat agcgtctaca
    11401 atcccctcta caagtaaatt gggtattata cttgtatata aacttccagg acccataatt
    11461 ataatgtcag catcataaat agatgcaata acatccttta atggttttac atcttttgga
    11521 tttaaaaaaa ttttatcaat agagcttttt tgaatttttg actcttcagg tatactggat
    11581 tctccattta ttatattgcc attttctaat ttagcaacta aatttacatc ttccaaagtt
    11641 actggtaaaa ctcttcctgt tatagcaaaa atttcactta atttatatac agctttttca
    11701 aagtttccat acaacccatt catagcagcc aaaaacaaat ttccaaaact ctgacctttt
    11761 aaaagccctt ctgtaaacct atattgcata acttcattca ttgttggctc tatatttgca
    11821 agtgctaata gacagtttct tatgtctcct ggtgggagca ttcctaagtc ttctctcaat
    11881 acacctgaac ctccaccatc atcagcaaca gttacaattg cagttatatt ttgcgtagtg
    11941 tgtttaagac ctcttaaaaa taccgattga ccagtacctc cacctatcac aaccacattt
    12001 ggttctctat tttcaacaat agacctttga aatagtttta ctttttcatt ttttgctttt
    12061 ttatatataa ataacgatac aattaatgca atccaaccta aaattcctac aataataagt
    12121 aatttagcca tgtttgcctc cattatcgca acatagagtc tctatgtttc tttacaactc
    12181 tatatccctt attagataaa tcctccgcta taagatttgt tatagtaact gacctatgtc
    12241 ttccacctgt gcatccaaca ccaattacta gatgttgttt tccttcctct atatattgag
    12301 gtactaaaaa atgaatcata tctaaaagtt tcacataaaa ttcttcactg attttagaat
    12361 tcataacata atctctaact tctttatcat caccagtttt agctcttaac tcttcaacat
    12421 aatagggatt tggaagaaat cttacatcaa atactaaatc tgcatcagca agtataccat
    12481 gtttaaatcc aaaagatact acagaaatag ttaggtttgg attatcttca ccagaagaat
    12541 atattttctt tatttcttcc ttaaggtctt taggtttcat gttagttgta tcaattatac
    12601 atgtagataa ctcctttaaa ggttccatta tctttctttc catttttata ccttctggaa
    12661 tctgcatatc ttttgccaat ggatgatttc ttcttgtcat tttatatctc tttaacaata
    12721 catcatcatt acaatccaaa tataccattt catattcata attttctttt tctaaataat
    12781 ttaagctttc atgaagcgct tcaaaaaact ttctccctct tatatctatc cctagagcaa
    12841 ctttatcaat acttgaattt ggttgataac ataattctgc aaatttagtt atcaaagcag
    12901 gaggtagatt atctacacaa taaaatccca tatcctctaa agctctcatt gtctcacttt
    12961 ttccagatcc tgaaagacct gtaactataa caaatttcat tgctaactcc tcccttttat
    13021 cttatgttta ttatattata atgttttcta ctaatgatat atccagatta gagtcagata
    13081 tagtcttcat agctagttca aaatcaccaa ctctttctgg acaatgtgca tctgagccta
    13141 ttataaattt attttctagc ccttttattt tatttaattg atttacattc aaaaaagaat
    13201 ggctactatt tatttcaagt ctagtatttg tcttttttgc tgtctttgct acttcttcta
    13261 tatacacatc gcctttatct cctggatgag taattataaa aatatcatta ttacttatcg
    13321 catttattac agcttttgta ttatattctt ttgctttttc agttttaaaa atatagttat
    13381 tacaatgatt taataagctt cttaatgatg ttggagtaga accaaagtga tatccagcca
    13441 taatataatc aaaatctcta ataaatttat catccatatc tatatttcct ttatcatcta
    13501 atatgttaca ctccatacct aaaagtatat ttatatcatt gtacttatca tttaaataat
    13561 ctatttcttc tctcattttg agaatatctt ttttctttac accatatgca atatgtttat
    13621 aactatggtc agatattcct attgttttta ttcctttaga aatggctacc tttacattat
    13681 cctcaatagt ccctttgcca tgactatata atgtatgtgt atgataatca gccagtattt
    13741 tcatttgtta atcctctcca agtattttga cttcttcttc caacataacc ccaaacttgg
    13801 catatacagc actttttata acatatatta aatccaaaat atctttagca cttgcttctc
    13861 cttgattgac tacaaatcca caatgcttct cagatacttg tgctcctctt aaagtaagac
    13921 ctctcaatcc agtttcttct attaatttac ctgcaaaata tcctgttggt ctcttaaatg
    13981 tacttcctgc actagcaaaa tttaatggct gtttagttat tcttttcgtc gctaaatcat
    14041 ccattaaact ttttatttca tcataatttc cttcttgtaa ttctaattct gctgataaag
    14101 ctatatatcc gtttttcgat attatacttt ttctataccc aaattccatc tgttcatttg
    14161 ataattcaaa tatattgcct tccatatcca tcaatcttac tgacttcact atgtccttca
    14221 tttcaccacc atatgctcca gcattcatgg ctaatgctcc tcccaatgtg ccaggtattc
    14281 ctgatgcaaa ctcaaaccct tttaattctt gcttttggag agctttccct aaaactgata
    14341 ataaagctcc tgactgagca gtcatcctag tatcatttat ctcataagaa ttgaaattat
    14401 cagctaattc tattaccact cctcttattc caccatcttt aattaaaatg tttgaaccat
    14461 ttccaattat taaatacgga atagattctt cttttaccaa cttaagtaca ttttttaatt
    14521 gttcttcagt tctaggtcta actaatatat ctgctggtcc tcctacccta aatgaaatat
    14581 gttttttcat aggttcatcg acttttatat cctctttact taatatattt aataaattta
    14641 tatatatatc ttgattattc attgtatttc ctcccaaatt atactgaact ttagctgtca
    14701 ttattattta aattatatta gtatatagta ttaactatat tcaataatat atgattttgc
    14761 ttatcatatg gattttatat tttaagcttc ttaattacaa actatattca ataaatatat
    14821 aataaagtaa aaaaaaaaaa ataacaacat ataaaagaat ataaataatt taaaaaaagc
    14881 atcaatatca ctgatatcaa tgctttaaaa ggcaatttaa ttcgtattat ttatctaact
    14941 atttttgaca tagcaaagat aataaatccc tccaactact atggaacctc caacgatatt
    15001 tccaagtgtt acaaatacca aattagttat aaattgaacc atagttatat ttgctcctaa
    15061 tgccattccc attggtataa aaaacatgtt tgcaacacta tgttggaatc cacataatac
    15121 aaatagcatt attactgtcc aacatgaagc caattttgac attatatctt gagatgctgt
    15181 agccatccat acagctaaaa cgactagaat attacaaagt attgctttta aaaacattga
    15241 tgctatagtt gaattaacct tagcttctgc cacagctata gcttttgtag ctgcatctcc
    15301 tgtcaatgta gaagaataaa acacaagtgc aactagcaaa attgaaccta ctaaatttgc
    15361 aaaataaacc agtccccagt ttcttaacat ttttcctaat gttatttttt tgtccataag
    15421 tgcaatagcc attaaggtgt ttccagtgaa taattctgct cctgccacta ctacaagcat
    15481 aagacctact ggaaagacca tagcacttaa aaagctagct acccctgggt ctatcttgcc
    15541 aaaagtttga ctaactatca tactccccaa tgccccaaaa cctataaatg ctcctccaaa
    15601 taaacctaat agaattgcgt tttgtgtcga aatatttgct tttttaatgc ctgcacttat
    15661 agttgcttgt gcagtttccc caggtgtcaa gaatccttta ctcataaatc cttccccctt
    15721 aaaaattata ctttcccttg tctttttatg gcaataatta tataataaca atatgatata
    15781 taataaattt tattaacata aaaaatatac tatatactta
//
LOCUS       DBGJFF010000088        15398 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.089, whole genome shotgun sequence.
ACCESSION   DBGJFF010000088 DBGJFF010000000
VERSION     DBGJFF010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15398
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.089"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            100..2601
                     /locus_tag="ACOIAF_002679"
     CDS             100..2601
                     /locus_tag="ACOIAF_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HGZ2669683.1"
                     /translation="MSSNKVVESYKITGMTCAACAKAVERVTKKMDGVYDQSVNIATE
                     KLKIEYDNSKVNFDDIKQVVEKAGYGIIKEESNKKIDMKIDGMTCAACAKAVERVVKK
                     LDGVESISVNIATDKANIDYDPSKVKLSQIKAAIEKAGYKPIEEVRNKVDVDEDKLRK
                     EREMKSLFVKFIVAIVFAVPLFYIAMGPMIIKPIGPWPLPEIINPMTNTFNYALIQLI
                     LVIPVMIAGYKFYINGFKSLFSLSPNMDSLVAIGTLAAFLYSLYTTLQIANGQIQGMH
                     HHQLYYESAGIIIALILLGKYLESKSKGKTSEAIKKLMGLQPKTAIVLVDGKEVETPI
                     EEVEIGDILLVKPGTKIPVDGVVIEGYTSVDESMLTGESIPVEKNVGSKVTGASINKN
                     GVIKFKAEKIGGDTALAQIIKLVEDAQGTKAPIAKLADTVSGYFVPIVIAIAVVASLL
                     WFLIGGKDIVFVLTIFISVLVIACPCALGLATPTAIMVGTGKGAENGILIKGGEALES
                     AHKVNTVIFDKTGTITEGKPKVTDIVLNNNVKEEYLIKIASSAEKGSEHPLGEAIVKY
                     GEEKNIKFEKVDNFKAIPGAGIQVTINDESILLGNRKLMNDNNIKLGDLEEKSNILAS
                     QGKTPMYIAVDGNLSGIIAVADVVKESSKKAIEILHDMGIKVAMVTGDNAKTANAIAN
                     QVGIDMVLAEVLPEDKSKEVEKLQNQGKFVAMVGDGINDAPALAKADIGIAIGSGTDV
                     AIESADIVLMKSDLIDVPTAIKLSHETIKNIKQNLFWAFGYNTIGIPVAAGILYVFGG
                     PLLNPMIAAAAMSLSSVSVVSNALRLKNFKAYKRD"
     gene            2761..3429
                     /locus_tag="ACOIAF_002680"
     CDS             2761..3429
                     /locus_tag="ACOIAF_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGZ2669684.1"
                     /translation="MNSSILVIEDDSNIQELISEFLSAEGYQVDTANDGLEGIQKFKQ
                     GSYDLVILDIMMPNLDGYGVCKMIRKSSSVPIIFLTALNDEGDQLKGFDLECDDYITK
                     PFSFNLLIKRVEAILRRSNKTINDKFIVFEKLKLDLNTYIAEIDGEPIELTLKEFNIL
                     KALIEKYPQVITREGLLDSIWGYDYYGDTRIVDAHIKNIRKKISLPYIKTVKGIGYTL
                     EKDI"
     gene            3413..4690
                     /locus_tag="ACOIAF_002681"
     CDS             3413..4690
                     /locus_tag="ACOIAF_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HGZ2669685.1"
                     /translation="MRRIFDKWEKLSIKYKLFSITTSLLIALALIIYLILYFLLPSYY
                     HEYKIESLQESLKSLVDSSIHFDTYTLEERLYYMAKDQNLAILLKDNQGKIVYGKNEV
                     VILRYSKYMINSLEDEYRTSIPIYTKDAKDGPYTLELVMPLQPIDEANEVIRKLMPYI
                     ISIAILIAIIGAYIYSIVITKPLINIIESEREQEYRRKDFVATISHELKTPITIISGQ
                     IEGMIYSVGKYKDRDTYLKKSYECTQELKDLVNEMIEVSKSEILEKDLKLVSINISEL
                     LNRLVKRQVFLIEEKHMKTILKIEENLEIKADQERITKAINNIINNAIKYSPEESELI
                     IRLYDKNKRISKKTSNQRVVLEIENTGVTIEKRYLEEIFNPFYRIEKSRSRKTGGSGL
                     GLYIVSQIFKSHGFDYSIKNKENSVVFTVEFKN"
     gene            5091..5312
                     /locus_tag="ACOIAF_002682"
     CDS             5091..5312
                     /locus_tag="ACOIAF_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669686.1"
                     /translation="MDRLKRDMKNMKEDLKEGVKKTAYVMKNTAEDLKDDAQGAMVNL
                     EMKKDEMMERYEQKKFSKELEKQMKNEMK"
     gene            complement(5601..6365)
                     /locus_tag="ACOIAF_002683"
     CDS             complement(5601..6365)
                     /locus_tag="ACOIAF_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HGZ2669687.1"
                     /translation="MFAEERIQAILNILKKEGRVTVKELSSKFNVTEDCIRKDLKNIE
                     KISSIRRIYGGAVLARETLENQDTKDRKEINIPTKKIIAEKAFNLIEDRETIFLDIST
                     INILLAKLLAESNKKVTLVTNMLDILNVVTSKPNNLNIISTGGLLNLSLDGFVGTPTI
                     EFISKYKFDKTFMGSCGVDVFNSSLTTFEIEDGLTKKAIINSSKKVFIVMEDKKFKFD
                     GNFKFAHLEDISSIITEKTPTPDIVDILSEFNVNVL"
     gene            6639..7313
                     /gene="fsa"
                     /locus_tag="ACOIAF_002684"
     CDS             6639..7313
                     /gene="fsa"
                     /locus_tag="ACOIAF_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HGZ2669688.1"
                     /translation="MQLILDTGNMEEIKELCTCLPIDGVTTNPSIVSKEKKNFKQLIN
                     EIGEIIGEDMPIHAQVLSTKYEEILEEALYISSLRKNIYVKIPVTQDGLRAIKDLHRK
                     GVKITATAIFTAHQGFLAAKAGANYIAPYVNRLDNISGDGVAMVSELIKIIDTYKMDT
                     KVLAASFKNAQQVIELMQHGVHSVTVPYDICKSMMNHPLTDWSVDKFIEDWENTFGKG
                     SKTNNI"
     gene            complement(7435..8829)
                     /locus_tag="ACOIAF_002685"
     CDS             complement(7435..8829)
                     /locus_tag="ACOIAF_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HGZ2669689.1"
                     /translation="MGIILKPKQFYMTVIATIILYFSLNYSFNIKFSICLSFLTILIW
                     AVDSVEKTFVALFFVILAFIFNIAPIKVILQFLFTENFYIIILAYIITNAVTKTGVAK
                     IISEKLILNTVNTPKKMILLSYILGILLIFFIPQPFPRVILVSAFYKEFLKEQQITED
                     SKSILLFSIFTASTFTSMFFVNGDTLLNYVVLELGNCNINWGQWAFYMSVPTIITCFI
                     TYFLFIFVFKKELHSIKFISNENLEGNKLHFSYSNLKKEFNLLNKEQKHVFLCLGIMF
                     FMFLTQFIHNINTLIIMSICVLLLLLRKIVGFSTLKEINWKVLIFFIAAFSIGGVLKY
                     SGVVDIMGNYLIKFIPNSSEIISILFLITLTIILNICLGSAVTTSSVVIPLLGSLHIL
                     KENSVTLCLFVYIIVSIQYILPFHHATIMVGHGENLYNSKVIFKYGLTLIPLTYFIII
                     CITFPWWKFIGLEI"
     gene            complement(8907..10253)
                     /locus_tag="ACOIAF_002686"
     CDS             complement(8907..10253)
                     /locus_tag="ACOIAF_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HGZ2669690.1"
                     /translation="MKDLTTGHEGKSIFFFAMPMLIGSLFQQLYNTADSIIVGRFIGK
                     EAMAAVSGANPIMFLLVAALMGVSLGFSILVSQFYGSGDLKKVKATIDTTYILLFIGS
                     ILISILGIVFGGPMLKLMNTPESIFAQSKLYLTIIFSGILFSAGYNSVSAILRGLGDS
                     VTPLYFLIIATILNIVLDLTFIVVLRMGVEGVALATIMAQAVSFIISIIYLNKRHEVL
                     KFKIKGIVYDNKIFKDGLRLGLPSGVQQMLFSIGNMALQFLVNSYGTSAMAAFGAGLR
                     IENFISLPIMNLGSAVSTFVAQNIGAGENERVKKGIRESIKMTLVLAVTVIALILLFR
                     ENLIALFNTDKDVIKIGSSYLFIIGPFFLFIGTSFVLSSAMKGAGDSMFALISSIVSL
                     WLGRLPASYMLSKFFGTDGIWMGIPFGWTLGLIVTVIYYKKGHWKTKAIVNHRINE"
     regulatory      10503..10605
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            10699..12075
                     /locus_tag="ACOIAF_002687"
     CDS             10699..12075
                     /locus_tag="ACOIAF_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HGZ2669691.1"
                     /translation="MQTTNSSTMQKLFPILTNKNVDMKKEIIAGVTTFLTMAYIIAVN
                     PNILSETGMPAGALVTGTCLAAAFGCILMGVVANLPFALASGMGLNAFFAYTVVLQMG
                     VPWEVALTAVFVEGIIFIVLSVSGVREAVVNAIPKNMKLAVTGGIGIFIALIGLVNSG
                     IVIGDQATLIKMGRFTPAVIITCVGLIIIAVLDKKRVKGSILFGIVVSSLLAWAFAFI
                     NPEYAQKLGIYLPGGLFKFESLAPIAGKIDLGYVLHPTNIGGFLVVVCTFLFVDFFDT
                     VGTLVGVCSKANMLDEKGNVPNVGRALLTDAVATTVGAGLGVSTVTTYVESSTGVIAG
                     GRTGWTAITVGILFLAAMFFSPVFIAIPSCATAPALIYVGYLMLGTVKDIEFDNITEG
                     VPAFVTIACMALTYSIGDGLTLGILTYVFVNIFYNIFGAKKAEDKKHVSVVMIVLAIL
                     FIIKLWLL"
     gene            complement(12189..13334)
                     /locus_tag="ACOIAF_002688"
     CDS             complement(12189..13334)
                     /locus_tag="ACOIAF_002688"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HGZ2669692.1"
                     /translation="MNTFEQLKISSTLIDGLKKQDITSPTEVQSLVIGNIIQNKDLLI
                     NSQTGTGKTLAYLLPIFEKIDTSKRETQALILAPTHELVMQITNQVELLAKNAELSVT
                     SLALIGEVNIQKQIKNIKAVKPHIVIGSCGRVLDLIKQKKLKSHNIKTIVLDEVDNLL
                     NGKNITCIEDIIRTTLRDRQIIGCSASLTDSTIKICDKLMKEFEIIKTKEKSQINPNI
                     NHSYLLGEIRDKFTFLRKALAATNPKKAIVFVNNEKNIEVLVSKLNYHNYKAIGIFGN
                     MEKEDRKNAINKFKLGKAKILITTDLSARGLDIVDVSHVFNLDFPKSKNEYLHRCGRT
                     ARGNRSGNTISIITKKELDIIKDLQKEFNIVITPKTLQNGELIDIIK"
     gene            13587..14294
                     /locus_tag="ACOIAF_002689"
     CDS             13587..14294
                     /locus_tag="ACOIAF_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2669693.1"
                     /translation="MLKVINVSKSYKNKKVVSSVSFDVNDGELFGFIGHNGAGKTTTI
                     KAIVGIHDFEEGEVLINSKSIKKQPVECKKEMAYIPDNPDLYESLTGIQYLNFIADIF
                     EVAKDKREELIKYYSNKFELNKALGDLISSYSHGMKQKLAIISALIHSPKILILDEPF
                     VGLDPKASFILKEIMREFCDKGGCIFFSTHVLEVAEKICDKIAIIKDGNIIAYGTTEE
                     VKGSNSLESIFMELIEK"
     gene            14341..>15398
                     /locus_tag="ACOIAF_002690"
     CDS             14341..>15398
                     /locus_tag="ACOIAF_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2669694.1"
                     /translation="MSNLSILIKNNIINEFKLNALKKADGREKQKIMVMMLSVVIIAG
                     LLIFYVTSLSLSLVDILKQINQLEMLLIFGFGLSTLTTIVTSLYKTSSYLFQAKDLDL
                     LTSLPIKESTVLASKILMLVGANYLFSSICMIIPAVVYFLNSDVSITYFPYLILLFLA
                     LPLLPIVASSIIFLFIGQISSRIKYKNLVLIIGSIILMLLYTLLMSRLNSMSVEMLKN
                     SVSISETIRKVYPPIYYFVDALKTGSIVSFLAFIAISIIPFFMFVTLFAKKFKDINAK
                     MSEGYKSKNYEFKGQKTSRPSKALLKKEFKRYTSSFIYFLNSSAGLLFLIVGTILILL
                     FGADKVGELLKLNIDFNL"
BASE COUNT         5917 a         1835 c         2388 g         5258 t
ORIGIN      
        1 aaatatagat ttctaaataa gtaatttatc aatattacat atcaaaataa aacgttacaa
       61 attagctgaa aatatattga aagaaaggaa gattttatta tgagttcaaa taaagtggta
      121 gaaagttata aaattacagg tatgacatgt gcagcttgtg caaaggcagt agagagagta
      181 actaagaaga tggatggagt atatgaccaa agtgtaaata tagctactga aaaacttaaa
      241 atagaatatg ataattctaa ggttaatttt gatgatataa agcaggttgt agaaaaagca
      301 ggatatggaa ttataaagga agaaagtaat aaaaaaattg atatgaaaat tgatggaatg
      361 acatgtgcag cttgtgcaaa agctgtagag agagtggtaa aaaaattgga tggagtagaa
      421 agtattagtg tgaatattgc aacagataaa gctaatatag actatgaccc atcaaaagta
      481 aaattatctc aaataaaagc agctattgaa aaagctggat ataaaccaat agaagaggta
      541 agaaataagg ttgatgtgga tgaagataaa ttgagaaaag aaagagaaat gaagtcttta
      601 tttgttaaat ttatagttgc aatagtattt gcagtacctc tgttttatat agcaatggga
      661 cctatgataa taaagcctat agggccatgg cctttgccag aaattataaa tcctatgaca
      721 aatactttta attatgcatt aattcagtta attttagtaa taccagtaat gatagctggt
      781 tataaatttt atataaatgg gtttaaatca cttttttctt taagccctaa tatggattct
      841 ttagttgcaa taggaacatt agccgcattt ttatatagtc tatatacgac actacaaata
      901 gccaatggac aaattcaagg tatgcaccat catcagttgt attatgaaag tgcaggaata
      961 ataatagcac ttatcttact tggtaaatat ttagaatcaa aatcaaaagg aaaaacatca
     1021 gaagcaatta aaaagcttat ggggctacaa cctaaaactg caatagtgtt agttgatggt
     1081 aaagaagttg aaactcctat agaagaagta gagataggag atatactatt agtaaaacca
     1141 ggaactaaaa tacccgtgga tggagttgta atagaaggat atacatcagt tgatgaatca
     1201 atgcttacag gtgaaagtat accagtagaa aagaatgttg gaagtaaggt gacaggagct
     1261 agtattaata aaaatggtgt tattaagttt aaggctgaaa aaattggtgg agatactgct
     1321 cttgcccaaa taataaagtt agttgaagat gcacaaggta caaaagcacc tattgctaaa
     1381 cttgccgata ctgtatctgg atattttgta ccaatagtaa ttgcaatagc tgtagttgca
     1441 tcattattgt ggtttttaat aggaggaaaa gatatagtat ttgtacttac tatatttata
     1501 tcagtacttg taattgcttg tccttgtgca ttaggattag caacaccaac tgctataatg
     1561 gttgggactg gaaagggcgc agaaaatgga atcttgataa aaggtggaga ggcactagaa
     1621 tctgcacata aagtaaatac agtgatattt gataaaacag gtactataac agaaggaaag
     1681 ccaaaagtaa cagatatagt attaaataat aatgtaaaag aagaatatct tatcaagatt
     1741 gcttcaagtg cagaaaaagg ttcagagcat cctcttggag aagcaatagt taagtatgga
     1801 gaagagaaga atataaaatt tgaaaaagtt gataatttta aagccatacc aggtgctggt
     1861 atacaagtta ctattaatga tgaaagtata ttacttggaa atagaaagct tatgaatgac
     1921 aataatataa agcttgggga tttagaagaa aaatcaaata tattggcaag ccaaggtaaa
     1981 acacctatgt atatagctgt agatggaaat ttatcaggta taatagcagt tgcagatgta
     2041 gtcaaagaaa gtagtaaaaa agcaatagaa atacttcatg atatgggaat aaaagttgca
     2101 atggtaactg gagataatgc gaaaactgca aatgctatag caaatcaagt aggtatagat
     2161 atggttttgg cagaggtttt accagaagat aagtcaaaag aagttgaaaa actacaaaat
     2221 caaggaaagt ttgttgcaat ggtaggggat ggaataaatg atgccccagc tcttgcaaaa
     2281 gcagatatag ggatagcaat aggaagtgga acggatgtag ccattgaatc tgcggatata
     2341 gtacttatga agagtgattt aattgatgtt ccaacagcaa ttaaacttag tcatgaaact
     2401 ataaaaaata taaaacaaaa cctattttgg gcatttggat ataatacaat tggtatacct
     2461 gtagcagcag gaatattata tgtatttgga ggacctttat taaacccaat gattgcagca
     2521 gcagcaatga gcttaagttc agtttcagtc gtatcaaatg cacttagact taaaaacttc
     2581 aaggcatata agagagatta gattaataaa ttaaataaaa tgtatccaaa agagctatct
     2641 ttaaattaaa aggattaatt tacaaattaa gatagctctt ttttcatgta aaattcacaa
     2701 caataagata aaatataagt aatatacgaa attttaaata aaatagaaag gattgataga
     2761 atgaattcgt caatacttgt aattgaagat gattcaaata tacaagagtt aatatcagaa
     2821 tttttaagtg cagaaggtta tcaagttgat acagcaaatg atgggttgga aggtatacaa
     2881 aagtttaagc aaggaagtta tgatttagtc atattagata taatgatgcc aaacttagat
     2941 ggttatggag tttgtaaaat gataagaaag tcatcgagcg ttccaattat atttttaact
     3001 gctttaaatg atgaaggaga ccaacttaaa gggtttgact tagaatgtga tgattatata
     3061 acaaaaccat tttcatttaa tcttcttata aaaagagtag aagcaatttt aaggcgaagt
     3121 aacaagacta taaatgacaa atttatagtt tttgaaaaat taaaattaga tttaaacaca
     3181 tatattgcag agatagatgg agaacctata gaacttacac taaaagagtt taatatatta
     3241 aaagctttga tagaaaagta tccacaagtt ataactaggg aaggtctttt ggatagtata
     3301 tggggttatg actattatgg agatactagg attgttgatg cacatataaa aaatataaga
     3361 aaaaaaatat cattaccata tattaaaact gtaaaaggaa taggatatac acttgagaag
     3421 gatatttgat aagtgggaaa agttaagtat aaagtataag ttattttcaa taacgacatc
     3481 attattaata gcattagctt taataatata tctaatttta tattttttat tgccatcata
     3541 ttatcatgag tataaaattg agtctcttca agaaagtttg aaatctttag tagatagttc
     3601 aattcatttc gatacatata ctttagaaga aagactttat tatatggcta aagaccaaaa
     3661 tttggctatt ttattaaaag ataaccaagg taaaattgta tatggtaaga atgaagtagt
     3721 tattttaaga tatagtaagt acatgattaa tagtcttgag gatgagtata gaacttctat
     3781 acctatatat actaaagatg caaaagatgg tccatatact ttagaactcg ttatgccatt
     3841 acaaccaatt gacgaagcaa acgaagttat aagaaaacta atgccataca tcatatcaat
     3901 agcaatattg atagcgatta ttggagcata tatatattca attgtaataa caaagcctct
     3961 tataaacata atagagagtg agagagaaca ggaatataga aggaaggatt ttgttgctac
     4021 aatatcacat gaactaaaaa ctccaataac cataataagt ggtcaaattg aaggtatgat
     4081 ttatagtgta ggtaagtata aggataggga tacttatctg aaaaaatcgt atgagtgtac
     4141 acaagaatta aaagatttag taaatgaaat gatagaggtt tcaaaatcag aaatacttga
     4201 aaaagatttg aaattggtat caataaatat aagtgaatta ttaaatagac ttgtaaagag
     4261 acaagtattt ttaatagaag aaaaacatat gaaaactatt ttaaaaatag aagaaaattt
     4321 ggaaattaag gcagaccaag agagaataac taaggcaatc aataatataa taaataatgc
     4381 cataaaatat tctcctgaag aatcagagct tataattaga ctctatgata aaaataaacg
     4441 aataagtaaa aagacttcta atcaaagagt ggttttagaa attgaaaata ctggtgtaac
     4501 tatcgagaaa cgttatttag aagaaatatt taatccattt tatagaatag aaaaatctcg
     4561 ttcgagaaaa actggaggaa gtggacttgg actttatata gtcagccaaa tttttaaaag
     4621 tcatgggttt gattatagca taaaaaacaa agaaaactct gtagtattca cagtagaatt
     4681 taaaaattaa ttacttatta taaagaatat ataactttca ataatagttc attttaaaat
     4741 cacaaaaaat tcataatgaa actgtattat ataaatataa agaagcttaa aaccataaaa
     4801 tacaccaact ataatgatga gatttcctta catcctccat ataagtcatc attatagtaa
     4861 cccaacaatt aagttactat gaatttaaaa aataacaccg agcatataat ctatgcttag
     4921 tgttattttt attttgaaat aaaaaatatt taatttattg aaatgtatag atttagatag
     4981 tttaaactat atttaagact taaaaattat aattgatatt acaaataaat atatgtactt
     5041 tgggaaaaat aaatatagga agtattatat ttacagagga ggtagttttg atggatagat
     5101 taaaaagaga tatgaaaaat atgaaagaag atttaaaaga aggtgttaaa aagacagctt
     5161 atgttatgaa aaatacagct gaagatttga aagatgatgc acaaggtgct atggtaaact
     5221 tagaaatgaa aaaagatgag atgatggaaa gatatgaaca aaagaaattt tctaaagaat
     5281 tagagaagca aatgaagaat gaaatgaaat aagcttttat ttatgaataa caaattattg
     5341 tattcatata aaaagtagcc agtattatta aatagtttat ttaaggaaat agactttcta
     5401 aaaataactg tattagattt agaaagtcta tttcaaatta gttttataaa tagacataag
     5461 gataaagtag atgttataga actagatttt aaaaaataga tactaaggat aaaataaata
     5521 ttatagaaat atgtttttaa gaatagttat caaataaaaa tttatattta taagaaatcc
     5581 tataaaatat ataattaaac ttataaaaca tttacattaa attctgataa aatatctact
     5641 atatcaggag taggcgtttt ttctgtaatt atagaactaa tatcttctaa atgagcaaac
     5701 ttgaaatttc catcaaattt aaacttttta tcttccataa ctataaatac ttttttactt
     5761 gaatttatta tagccttctt cgtaagacca tcttcaattt caaaagttgt aagtgaagag
     5821 ttgaatacat ctactccaca acttcccata aaagtcttat caaacttata tttcgatata
     5881 aactctatag ttggagtacc gacaaaaccg tctaaactga gatttaataa acctccagtg
     5941 gaaattatat ttaaattatt tggttttgaa gtaactacat ttagtatatc cagcatattt
     6001 gtaacaagtg ttactttttt attactttca gctaacaact tcgcaagcaa gatgttaatt
     6061 gtagagatat ctaaaaatat agtttctcta tcttcaatta aattaaatgc tttttctgct
     6121 attattttct ttgttggaat attaatttct tttctgtctt ttgtatcctg attctctagt
     6181 gtttctctag ctagtacagc tcctccataa attcttctta ttgaagaaat cttttctata
     6241 ttttttaaat cttttcttat acaatcttct gtaacattaa atttagaact gagctctttg
     6301 acagtaactc taccttcttt ttttaggata tttaatatag cttggattct ttcttctgca
     6361 aacattttct acctcttttc gcaatatagt attatagtaa atattataag ataaaataca
     6421 atatatcaac tattttaaca tgaaatacaa aattaattta atataaaaag tatgtttttt
     6481 ctaactatat atttagaatt tagtaaataa aaaaattata aaactaagaa ggaaaagtaa
     6541 aacaataata aaaaataaaa aacaataagc aaaatgttga aaataatacc aaatcaatat
     6601 ataataagaa taaagaaaga ttaaaggggg atattttaat gcaactaatc ttagatacag
     6661 gtaatatgga ggagattaag gagttatgta cttgtttacc aatagatggt gtaactacaa
     6721 atccaagtat tgtttcaaaa gaaaaaaaga attttaagca acttataaat gaaattggtg
     6781 agattatagg tgaggatatg cctatacatg ctcaagtgtt aagtacaaag tatgaagaaa
     6841 tattagaaga agcattatat ataagtagtt tgagaaaaaa tatatatgta aaaatacctg
     6901 taacacaaga tggtttaaga gctataaaag accttcacag aaaaggtgta aaaataactg
     6961 ctactgcaat attcactgct catcaaggtt ttttagctgc aaaggctgga gcaaattata
     7021 ttgcacctta tgtaaatagg cttgataata tatctggtga tggagtagct atggtttcag
     7081 aacttataaa aataatagat acttataaaa tggatacaaa agtacttgca gccagtttta
     7141 aaaatgcaca acaagttata gaattaatgc aacatggggt gcatagtgtt acagtaccat
     7201 atgatatttg taaatctatg atgaatcatc cattaactga ttggagtgta gataagttta
     7261 tagaggattg ggagaatact tttggaaaag gaagtaaaac aaataatatt tagtattatg
     7321 gtaaaagagt agttttgtcc aattttacaa gaacttatat aaaaagatga aaatatgaat
     7381 tttcttttta ataatttcat tattatctta agttaattca tattttcatc ttttttagat
     7441 ttctagtcct ataaatttcc accaagggaa tgttatacat attattataa agtaggttaa
     7501 aggaatcaaa gttaaaccgt atttgaaaat aaccttgcta ttatacagat tttcaccatg
     7561 accaaccatt attgtagcat gatgaaatgg taatatgtac tgtatactta ctataatgta
     7621 gacaaataga cataaagtaa cactattttc ctttagtata tgtaaacttc caagtaacgg
     7681 tattactaca gatgatgtag tgacagcact tccaagacat atattcaaga ttattgtgag
     7741 agttatcaaa aacaatattg aaataatttc tgaagagttt ggtataaact ttatcaaata
     7801 atttcccatt atatctacaa ctccagagta ctttaacact cctccaattg aaaatgctgc
     7861 tataaaaaaa ataaggacct tccaatttat ttcttttaaa gtactaaatc caactatttt
     7921 tcttagtaat aaaagaagta cacaaattga cattataatt aatgtgttta tgttatgtat
     7981 gaactgagtt aaaaacataa aaaacattat tcctaggcat aaaaatacgt gtttttgctc
     8041 tttgtttagc aaattaaatt ctttttttaa attagaatat gaaaaatgaa gtttattacc
     8101 ctcaagattt tcattagaaa taaattttat tgaatgcaat tcttttttaa agacaaagat
     8161 aaataaaaaa taagttataa aacaagtaat tatagttgga acagacatat aaaaagccca
     8221 ttgaccccaa tttatattac aatttccaag ttctagtaca acataattta gaagagtgtc
     8281 tccatttaca aaaaacattg atgtaaaggt agatgctgta aatatactaa ataaaagtat
     8341 agattttgaa tcttcagtta tctgttgttc ttttaaaaac tccttgtaaa aagcagatac
     8401 aagaataacc cttggaaatg gttgtgggat aaaaaatatt aaaagtatac ctaaaatata
     8461 cgataaaaga atcatttttt taggggtatt tacagtattt aaaattaact tctcagaaat
     8521 aatttttgcg acacctgttt ttgtaactgc attagtaatt atgtatgcaa gaattattat
     8581 gtaaaaattt tctgtaaata aaaactgtaa aataaccttt attggtgcaa tgttaaatat
     8641 aaacgctaga attacaaaaa acaatgcaac aaaagttttt tcaactgagt ctacagccca
     8701 tatcaagata gttaaaaaag ataagcatat agaaaatttt atgttgaaac tgtaattaag
     8761 agagaagtag agaattatgg tagctataac agtcatataa aattgttttg gcttaagaat
     8821 aatacccatt tttatattca cctccataat ttattatatc ataatggagg tgaatacaat
     8881 gaatgtgaat acattttata aaaagattat tcattgattc tatgatttac aatagcttta
     8941 gttttccaat gtcctttctt atagtagata actgtaacaa taagacctaa agtccaacca
     9001 aaaggaatac ccatccaaat tccgtctgtt ccaaaaaatt tagaaagcat ataagaagct
     9061 ggaagtcttc caagccataa agatactatt gagcttataa gtgcaaacat ggaatcacca
     9121 gcacctttca ttgcacttga aagtacaaaa gaagttccaa taaataaaaa gaatggtcct
     9181 attataaata agtaagaaga gccaatttta atgacatctt tatcagtatt gaatagagca
     9241 attagatttt ccctaaatag caaaattaaa gcaataactg taactgctaa aactaaggtc
     9301 atttttatag attctcgaat gcctttttta actctttcat tttctccagc acctatgttt
     9361 tgagcaacaa aagtagaaac agcagaacca agattcataa taggtaagct tataaaattt
     9421 tctatcctaa gaccagcacc aaaagctgcc atagcagatg taccataact attaactaaa
     9481 aattgaagag ccatatttcc aattgaaaac aacatttgtt gaactccact tggaagtcct
     9541 aatctaagtc catctttaaa tattttgtta tcatatacaa ttcctttgat tttaaattta
     9601 agcacttcat gtcttttatt taaataaatt atacttatta taaatgatac tgcctgagcc
     9661 attatagtgg caagtgcaac gccctcaaca cccattctta gcactacaat aaaagttaag
     9721 tctaaaacta tatttagaat tgtggctatt atcagaaaat aaagtggagt tactgaatct
     9781 ccaagtcctc taagtattgc acttactgaa ttatatccag cagaaaataa tattccacta
     9841 aatattatag taagatacaa ttttgattga gcaaaaatgg attctggtgt attcataagc
     9901 ttaagcatgg ggccaccaaa tactattcca agaatactta ttaatataga accaataaat
     9961 aaaagtatat atgtagtatc aatagtagct tttacctttt ttaaatctcc tgagccataa
    10021 aattgtgaca ctaaaataga aaatccaagg ctaacaccca taagcgctgc caccaataaa
    10081 aacattatag ggttagctcc agagactgcc gccattgctt cttttccaat aaatctacca
    10141 actattatac tgtcagctgt attgtatagt tgttgaaata aactaccaat aagcataggc
    10201 atggcaaaaa agaatatgga ctttccctca tgacctgtag ttaaatcttt caccttattc
    10261 ctccaaataa aaaaattatt aatttattat agtataatct taacataaac tttatatcca
    10321 aactataaat aaattgtatc aaaatatcat atattttttt aaaatgtaaa gaatataatt
    10381 ataatttgaa tttaataaat caatatttaa tataatcgaa tattaataca aaatcaatac
    10441 aatatatacg tatatattga caaaattaat tatataaatt aaaataatat tcgtaaagaa
    10501 aaattcatgt tttgtactgt ataacatata attttgacaa tatgggtcat aagtttctac
    10561 cggaataccg taaatattct gactatgtat actattattt agttaattaa atatttaatt
    10621 aactgaatta tgaaggatac ggctcagctt atcaactact gtgtcgtttt tttgtaaaat
    10681 ttaataggag gtgttgttat gcaaacaaca aacagtagta ctatgcagaa actttttcca
    10741 attttgacta ataaaaatgt cgacatgaag aaggaaataa ttgcaggagt aactactttt
    10801 ttaactatgg cttatataat agctgtaaat ccaaatattt tatctgagac aggcatgcct
    10861 gcaggtgcac ttgttacagg tacttgttta gcagcagcat ttggatgtat tttaatgggt
    10921 gtagttgcta accttccttt tgctttagca tcaggtatgg gtcttaatgc attttttgca
    10981 tatacagtag ttttacaaat gggtgttcct tgggaagtag ctcttacagc agtatttgta
    11041 gaaggtatta tttttatagt attatcagta agtggagttc gtgaggctgt tgtaaatgcg
    11101 ataccaaaaa atatgaaatt agctgttaca ggtggtatag gaatatttat tgcacttata
    11161 ggtctagtta acagtggaat tgttattgga gaccaagcca ctcttatcaa aatgggtaga
    11221 tttacaccag cagtaataat aacttgtgta ggattaataa ttattgctgt attagataaa
    11281 aaaagagtta aaggttctat actttttgga atagtagtga gttcattact tgcttgggca
    11341 tttgctttta taaatccaga gtatgcacaa aaattaggta tatatttacc tggtggatta
    11401 tttaaattcg aaagtttagc accaatagca ggtaaaatag atttaggata tgtactacat
    11461 ccaacaaata taggtggttt tttagtagta gtttgtacat ttttatttgt tgattttttt
    11521 gatacagtag gtacattagt tggtgtttgt tctaaagcaa acatgttaga tgaaaaaggg
    11581 aatgttccta atgtaggtag agccttactt acagatgcgg ttgcaactac agttggtgca
    11641 ggacttggag tatctacagt tacaacttat gttgagagtt caacaggagt tatcgcaggt
    11701 ggaagaactg gatggacagc aataacagtt ggaattttat tcttagctgc aatgtttttc
    11761 tctcctgtat ttatagcaat tccatcatgt gctacagcac cagctttaat atatgttgga
    11821 tatttaatgc ttggtacagt taaggatata gagtttgata atataactga aggtgttcca
    11881 gcttttgtta ctattgcttg tatggcatta acatatagta taggtgatgg tttaacttta
    11941 ggtatattaa cttatgtttt cgtaaatata ttttataata tatttggagc taaaaaggca
    12001 gaagacaaaa aacatgtttc tgtagtaatg attgttctgg caatattatt tataataaaa
    12061 ctttggctac tataataggt attatttaaa attttaattt aatataaaat aaagggctgt
    12121 aatttattat caattgtgat gataaactac agctcttttg tatttgttgt atttttgtat
    12181 ttattgtatt atttaataat gtctattagt tctccatttt gtaaagtttt aggtgtaatt
    12241 actatattga actctttttg caaatctttt ataatatcta attctttttt tgttattatt
    12301 gagattgtgt taccacttcg atttcctcta gcagttcgac cacatctatg caggtattca
    12361 tttttacttt ttggaaaatc caagttaaaa acatgactta catccacaat atctagtcca
    12421 cgagcagata aatcagttgt aattaaaatc tttgctttac caagtttaaa tttatttatt
    12481 gcattctttc tatcttcttt ttccatattt ccaaaaattc ctattgcttt gtaattatga
    12541 taatttagtt ttgatactag gacttctata tttttttcat tatttacaaa tacaatagct
    12601 ttttttggat ttgtagctgc taatgccttt ctaagaaaag taaatttatc ccttatctca
    12661 cctaataaat atgagtgatt gatgttagga tttatttgtg atttttcctt tgtttttatt
    12721 atttcaaact ccttcataag tttatcacaa atttttatag tgctatcagt aagacttgca
    12781 gagcatccta taatttgtct atctcttaac gttgttctta ttatgtcttc tatacaagtt
    12841 atattttttc cattcaatag attgtccact tcatctaaga caatagtttt tatattatgt
    12901 gattttagtt tcttttgttt tatcaagtct aaaactcttc cacaagagcc aattactatg
    12961 tgtggtttta ctgcttttat attttttatt tgtttttgta tattgacctc cccaattaaa
    13021 gctaaagaag taacggagag ttctgcattt ttagccaata attcaacctg gtttgtaatc
    13081 tgcattacta attcatgcgt aggtgcaagt ataagagctt gtgtttctct ttttgatgta
    13141 tcaatttttt caaatatagg taaaaggtac gctagtgttt ttcctgttcc agtctgtgaa
    13201 ttaatcaata aatccttatt ttgaattata tttcctataa caagtgactg aacttctgtt
    13261 ggagaagtta tgtcttgttt tttgagtcca tctattagag tggaacttat ttttaattgt
    13321 tcaaatgtat tcatattttg ctccttatat tttaataatt atagtcaact attttttatt
    13381 atacattgtt tattcaaatc gaactatata ttcttttata attatgtgta atttgaaagt
    13441 aaatatcaat tatatttata cctttatatt tctgatacct ttgcatttta tagatttcta
    13501 aaataaacaa ataaaagttg taattgtttg atatttgtta tatattatat ataacaaata
    13561 tcaaaactat aatatggagg gaaattatgt taaaagtaat caatgtatct aaatcatata
    13621 aaaataaaaa agtagtaagt agtgtctcat ttgatgtaaa tgatggagag ctttttgggt
    13681 ttataggaca taatggagca ggtaaaacta ctactattaa agcaattgtt ggaatacacg
    13741 attttgaaga gggagaggta ctaataaatt ctaaatctat aaaaaaacag cctgttgaat
    13801 gtaaaaagga aatggcatat atacctgata atccagattt atacgagtca ttaactggta
    13861 tacaatacct taattttata gctgacatat ttgaagttgc caaagataaa agagaagagt
    13921 taataaaata ttattcaaat aagtttgaac tcaataaagc attaggagat ttaatatctt
    13981 catattcgca tggtatgaaa caaaaattag ctataatttc ggctttgata cacagcccca
    14041 aaatcttaat tcttgatgaa ccttttgtag gtcttgaccc aaaagcttcc tttatattga
    14101 aggaaattat gagggagttt tgtgacaaag gtggttgtat atttttctca acacacgttt
    14161 tagaagttgc tgaaaaaatt tgtgataaga ttgccataat aaaagatgga aatataattg
    14221 cttatggaac tacagaagaa gttaagggaa gtaattcact tgaaagtatc tttatggaat
    14281 taatagagaa ataaaatgtt ttttatacaa tatagtgtct aaagtgagga atgataaaat
    14341 atgagcaatc taagtatatt aataaaaaat aatataataa atgaatttaa attaaatgcg
    14401 ttaaaaaaag ctgatggtag agaaaaacaa aaaataatgg taatgatgct ttcagttgtt
    14461 ataatagcgg gtctattaat tttttatgta acgagcttaa gtctctcttt agttgatatc
    14521 ttaaagcaaa taaatcaact ggaaatgcta ctgatatttg gttttggatt atcaacatta
    14581 acaactattg ttacatcatt atataaaacg tcatcatatc tatttcaagc aaaagattta
    14641 gaccttttga ctagtttacc tataaaagaa tctactgtac tagcaagtaa gatattaatg
    14701 ttggttggag caaactattt attttcttca atatgtatga ttatacctgc agtggtttac
    14761 tttttaaatt ctgatgtaag tataacttat ttcccttatt tgatattgct atttttagct
    14821 ttaccattat taccgatagt tgcttcatca ataatttttc tatttatagg gcagatatca
    14881 tcaagaataa aatacaaaaa tttagtatta atcataggta gcattatatt gatgttgctt
    14941 tataccctac ttatgtcaag acttaattct atgtcagttg aaatgctaaa gaatagtgtg
    15001 tctatttcag agacgattag aaaagtatat cctcctatat attattttgt tgatgctctt
    15061 aaaacaggaa gtatagtttc atttttagca tttatagcca tctcaataat accatttttt
    15121 atgtttgtaa ctctgtttgc aaaaaaattt aaagatataa atgctaagat gagtgaagga
    15181 tataagtcaa aaaattatga atttaagggt caaaaaactt caagaccttc aaaagcttta
    15241 ttaaaaaaag aatttaagag atatacttct tcgtttatat actttttaaa ttcatctgca
    15301 ggtttgttat ttctaatagt aggcacaatt ttaatattat tatttggagc agataaagtg
    15361 ggtgagttac tgaaattgaa tatagatttt aatcttat
//
LOCUS       DBGJFF010000089        15336 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.090, whole genome shotgun sequence.
ACCESSION   DBGJFF010000089 DBGJFF010000000
VERSION     DBGJFF010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15336
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.090"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            305..994
                     /locus_tag="ACOIAF_002691"
     CDS             305..994
                     /locus_tag="ACOIAF_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HGZ2669695.1"
                     /translation="MLQKVLWKFLKLVVGLFICSVGIVLTINCNLGLSPWDVFHQGLS
                     NHIGITIGTASIIVGSIVVIADVVLGENVGWGTVFNMLLIGFFMDLLLYSNLIPEADS
                     LFVGIIMLILGLVLLSVGMVFYMGSGLGSGPRDGLMVAIQKKTGKSLKLIRGTIEVGA
                     LIVGFLLGGKVGIGTIISAFGLGYFTQMVFGLFKLDCSKIKHRFIVDDIKFIKVYMSG
                     EKKAAVQNIVK"
     gene            1430..1969
                     /locus_tag="ACOIAF_002692"
     CDS             1430..1969
                     /locus_tag="ACOIAF_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HGZ2669696.1"
                     /translation="MDIGEKIKRLRTEKQLTQEELANRCELSKGFISQLENNLTSPSI
                     ATLIDILEILGTNLREFFNEIDDERISFTKEDMFETEDEDLKYKLKWLIPNSQKNEME
                     PIIITLYPGGQYKEEKPHEGEEFGYVLAGSIYVHIGEKKNKVKKGESFYFRPKANHYI
                     SNEGKTTAKVIWVSTPPSF"
     gene            1988..3031
                     /gene="potA"
                     /locus_tag="ACOIAF_002693"
     CDS             1988..3031
                     /gene="potA"
                     /locus_tag="ACOIAF_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:1902047 - polyamine transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein"
                     /protein_id="HGZ2669697.1"
                     /translation="MVENIIELRDISKHYEELTVLDNFNLDIKKNEFLTLLGPSGCGK
                     TTTLKIIAGFEYADDGKVLFEGKEINNLPPYERQVNTVFQKYALFPHMDVYENVAFGL
                     KIKKTPKDVIDAKVKEVLKLVALEGFERRQVESLSGGQQQRIAIARALVNEPKVLLLD
                     EPLGALDLKLRQEMQIELKRIQKKLGITFVFVTHDQEEALTMSDTIVVMYKGKIQQMG
                     TPQDIYNEPKNSFVAKFIGESNIFDGIMIEDYKVNFCNRDFECVDKGFEKNENIEVVI
                     RPEDIKMVKPEEGMLKGKVTSTVFKGVHYEIELNENGRLWILHNTKNAEVGTELGMDI
                     YPEDIHIMRKENN"
     gene            3033..3869
                     /locus_tag="ACOIAF_002694"
     CDS             3033..3869
                     /locus_tag="ACOIAF_002694"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2669698.1"
                     /translation="MKRKSFLAYPYVVWSAIFVIIPLILVVFFSFTKESGGGYAFTLE
                     NYKEVIDPIYMKVFGRSILLAGGATLICLIVGYPVAYIISKARVSRRGSLILLFILPM
                     WMNFLLRTYAWVAILGKNGLLNTFLGWFGIQPLAILYTNFAILLGMVYNFLPFMVLPI
                     YTALSKMDNDLINAAHDLGANNMTVFRKIIFPLSLPGVMSGITMVFMPAVTTFAISRL
                     LGGGKIMLVGDLIEQQFTVVGDWNFGSAISIFMMIVILISMSIMSKFGDESDKEGGGL
                     LF"
     gene            3974..4648
                     /locus_tag="ACOIAF_002695"
     CDS             3974..4648
                     /locus_tag="ACOIAF_002695"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2669699.1"
                     /translation="MARWNGFTWKWYGQLLQNESIMSALYYTIVIAILASVISTIVGT
                     ISAIGIHKMRGKSKKLILNVNYLPILNTEIVTAVALMSLFVFVKMEFGFTTMLLAHIM
                     FCLPYVILSVLPKMKQLPDNIEDAAMDLGATPIYALRKVILPQIKPGIVSGFLIAFTM
                     SIDDFIISFFNAGNGVSNLSIEIYGMARRGIKPEINALSTIMFAVVLGLLLLANKKES
                     IVRGIK"
     gene            4648..5700
                     /locus_tag="ACOIAF_002696"
     CDS             4648..5700
                     /locus_tag="ACOIAF_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HGZ2669700.1"
                     /translation="MIKFKKAISLIAVGMMIVTLFTGCNKATDSTKVLNVYNVGDYID
                     ESLIDKFEKETGIDVQYSTYDTNEMMYQKVKSGSTHYDLVFPSDYMVEKMKNEGLLEK
                     LDFKNIPNMKYLDKSFLNPVYDKTNEYSVAYMWGTLGILYNKKEVKDPMDSWNILWNP
                     KYKGNIMMFDSVRDTIGITLKKLGYSMNSVNPKEINEAKNLLMKQKDLVLAYVNDEGK
                     DRLLGGEVAMGMLYSGDAVTLMEQNPDLDYAIPKEGTNKWVDAMCVPTTAQNKKEAEL
                     FINFLLDPENAKVNAEYIGYSTPNTGALKLLDPEITQNPVAYPSKEVLDKCETFEDIG
                     ENIKLYDKAWIELKSK"
     gene            5930..6778
                     /locus_tag="ACOIAF_002697"
     CDS             5930..6778
                     /locus_tag="ACOIAF_002697"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HGZ2669701.1"
                     /translation="MNRINKKIDLLILALLVFIFLIVGIVILSIRTENKLNVFIMLAI
                     IFFIIMLTYLSNSVVGLITSSIIIFMYTSYILYNNITHNMDVEFISYMWIIATPVSSI
                     IMGNLNKSINELQNTNKKLSEQYKELVTIDSETGLRNLKIFYNDVNMEISKSIRHNTD
                     FSLMIVKLPYYGNLQTIFGENKTNKIVKYIGSNIIECTRNEDIIYSLQKDMIGILMPN
                     TSLEGSKVVKDRIKKRIKELNLDLNNRGKYVNIDVKIAFLQYKSSFGDSINFKNIVEE
                     ELQYDV"
     gene            6768..8432
                     /locus_tag="ACOIAF_002698"
     CDS             6768..8432
                     /locus_tag="ACOIAF_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2334 domain-containing protein"
                     /protein_id="HGZ2669702.1"
                     /translation="MMFKIFNKSLLKLVVVLSIVLTISNFSLSSYASEITSSTDKVLI
                     IYDSKKETAYNRDILNIMRTLLGRFSSDIELLKLSNYDGEINKNYYSHIFILGINENS
                     YNNDKNTKNLISSLNSYKGTICWLGYGIENLLEHKKYNLDYVGKTNNIVSVNYRGKSY
                     NLDEHYVFNIVESKDTSNKVIGSINDTLNKYPYIINDKNLFYVSKLDLDGVLFYIFCD
                     SLNDIFNIKTFDKGRIFVRIEDVHAFREPKNLVEIADYLSSKNIPFTIALIPAYVNPK
                     NHKVITLSESPEIVKAIKYMQDKGGTVILHGYTHQYKKEEVSGEGYEFWDGKKDEPLK
                     ENMKIFVKDRVLNGLRVCIENGIYPLAFEAPHYAMESEGYKELKKYFSTYMGQHQNND
                     KKFSTNTYPYIIRDTEEFNIFIPENLGYIDPEDKFTFQNIKENLDKLSIVRGFSGGFF
                     FHSYLNIEYLKNTIEYLEKQNIEFMNLRDFNNWVKVDEIQIRNNGDEIIVNYDKDLDE
                     ITKSDTRFKSISNISKILIFIVSISVLIFVIIFIYFKRIDKKKFLK"
     gene            8481..9737
                     /locus_tag="ACOIAF_002699"
     CDS             8481..9737
                     /locus_tag="ACOIAF_002699"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HGZ2669703.1"
                     /translation="MRDLNVSEYLFIFSLFSIWSLLLINIILAMGGYIFYFKNFDKEI
                     KEIDEYPMISILVPAHNEAKVIGRTVESLLLLNYPKSKMELIVINDNSSDNSKEILEN
                     IKDRYNNYNFTIINTDSLTGGKGKSNALNIGYTISKGDFIAVYDADNTPDKNALRYLV
                     QTIVMNDELGAVIGKFRTRNKNKNLLTKFINIETLSFQWMSQAGRWQLFNLCTIPGTN
                     FILRRSIIEEIGGWDSKAIAEDTEISFRIYKLGYKIKLVPQSITWEQEPETVKVWIKQ
                     RTRWAKGNIYVLMKYIKNIFKQGRNKIVFDIAYFFSVYFLFLTSVIISDILFVLSISK
                     LVEISIPINFFLIWILSYLLFIIEVSISLTIEKGEATIENIFIVAIMYFTYSQLWLFV
                     AIKGMIEYLKDIIFKREVKWYKTERF"
     gene            9760..11841
                     /locus_tag="ACOIAF_002700"
     CDS             9760..11841
                     /locus_tag="ACOIAF_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HGZ2669704.1"
                     /translation="MKKFIISIISLVLFFSNISLIYKVNADETKVKNYKFERDITIDG
                     VIGSNSTFFEVNKNWDIEEVLLHLNFSKSQILNGDVSSLTVLINNVPIKSIKLNAKTN
                     YKNTLEVLVPKDYIIQGYNEIKIKTYKTISDKICQDDSNTGNWMVIHKESYISIRYKQ
                     KKVENSINEYPYPYAEIENNHKLDTTIVVPDNMTRGETTAVFNLASAFGKITKNDDLK
                     LDVKLYSEMKNWSDDNIIYIGKPENTAEEILDILSIKEQTLLSSNCIIKQVDSPYNKN
                     KKMMVVIGSNEDDLIKASNLLIENRLSNQVLSSSVLVNKETNIKINREQKLNLGHLTL
                     KDLGYSDFLLEGAFNQQALFDVKIPTGKVLDDGSKIILNLRYSDNLDFEKSLVTVSIN
                     DVIVGSKKLDRSHSNNDKLELKIPKDIDNKNYYQVKLTFNLSIKNSNCVTRESNNPWA
                     YVSNNSYLALSTKENETLSFENYPYPFVRDDEFNDLTVIMPDYSGSQAMTWMFRLGVT
                     LGANINSHNGNINVIRGKEFSDKYKDTNIVVFGVPHNNSVIKMLNNNLNIKFDKNYSN
                     FISNDKISFIDDYGKNISTIQLIKSPYNNQKNIMVISSMNEKNLYLGMDYLLNKSKVN
                     DLKGDTLIIDEYGEVEDLAYNLKSKKEVKDSSWNMSINKTTKVFLMISFITIIVVMIL
                     SMLYIKKYKRR"
     gene            11878..12963
                     /locus_tag="ACOIAF_002701"
     CDS             11878..12963
                     /locus_tag="ACOIAF_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HGZ2669705.1"
                     /translation="MRKYMNKFKLKRSILISLIILIFIFIVYFVSIELKDRSLVNNQY
                     KGKIKSANLSVDYEINQVMKDIDKLGLNTVNVPIIINVESINSDIMSIDNNSKEKAIK
                     LIKKLNKKGISTILEAYPWIENGKLYETDWNPINKKRFFYTWQNAILNELIIDVANPL
                     DVNVLNIGSNFVHLEEYQQNWGEIIDFVQSKFDGLVTYRTNWWYTKKDDLSSKLFYEQ
                     KLNNNFFDKLDFISIAAYFELSNKPVNTVDELISALHSSTVNNRGQNIKQEIYNLYKA
                     HRKPIFFGELGFSNRESASSQPWNHTPSKAVNGEEQARCFEAYKKVFENEDWINGFSV
                     FCVGKIDDEKNFYPSKESIKVIKSWYE"
     gene            13017..14141
                     /gene="wecB"
                     /locus_tag="ACOIAF_002702"
     CDS             13017..14141
                     /gene="wecB"
                     /locus_tag="ACOIAF_002702"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HGZ2669706.1"
                     /translation="MNDIKVMTVFGTRPEAIKVAPLIKELEKRENIKSIVCVTAQHRE
                     MLDQVIETFNINVDYDLDIMEKGQSLNDITCKILNKLPLILNKENPNIILVHGDTTTT
                     LATSLTAFYNKTLVGHIEAGLRTYDKYSPFPEELNRQLTGIIADMHFAPTNLARKNLI
                     SEGKPNNNIFVTGNTAIDALKMTIKENYNHPIIDEIGNDRMILLTSHRRENLGKPMKN
                     IFRAIKRIVDDFEDVQIVYPIHLNPKIRTIADEIFGKFPEKIHIIEPLDVADFHNFLN
                     KSYMIMTDSGGIQEEAPSLGKPVLVLRDKTERTEGIEAKTLKLVGTNEDRIYNSVSDL
                     LINKDNYVQMSKASNPYGDGNASKYIVDIIIKKFNCKYLN"
     gene            14219..15193
                     /locus_tag="ACOIAF_002703"
     CDS             14219..15193
                     /locus_tag="ACOIAF_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HGZ2669707.1"
                     /translation="MARKLIKNLGKSKSVKRVKLLFKKIFITVFIVASIVAIFNITKY
                     FEELYKVRDLKSTKIEYYMDVADEAGDGKVQLSWKALLAIDMVIHDEDLSNIKKKDTL
                     DIGEKFIVEDKNGKGEKVYKVKKFNKVLSELKFDSSQKSRARKYMKDLEYTYLGNKQL
                     DSSDEKIKFIKKLEDSAIREYIDYGILPSITIGQAILESGWGNSKLTKQSNNLFGIKA
                     DKAWKGKSVEISTSEHYNEKIVASFRSYNSLQDSVKDHSLFLINNKRYRKHGLFEAKD
                     YISQAQALENAGYSTAEDKKGNRIYAELLIDVIRSYNLQLIDNKVETK"
BASE COUNT         6226 a         1453 c         2607 g         5050 t
ORIGIN      
        1 gtaataaaaa aactgtttaa gtataataac ttatatttag acagtttttt tcatttataa
       61 ataatataaa tgaaattgaa aacatttgaa ggatagaaac tggttggata aatgtataaa
      121 ttactttacc aaaaaataat gacaaaaatt gtaactaagt tgtaataaaa tggtagttat
      181 ttagcataaa ataatgtatc atagaagtat attataaaat taataaaata agatttttgt
      241 aagaaaactg ctggcttttg atatttagag tgagtagtta aattatgttt aggaggggta
      301 gaatttgttg caaaaagtat tatggaaatt tttaaagcta gtagtcggtt tatttatatg
      361 ttcagttggt atagtattga cgataaattg taaccttgga ttatctccct gggatgtatt
      421 tcatcaagga ctttcaaatc atataggaat aactatagga actgcttcta taattgtagg
      481 ttctatagta gttattgctg atgtcgttct tggagaaaat gtaggatggg gaactgtttt
      541 taacatgcta ctaattggat tttttatgga tttactttta tatagtaatt taattccaga
      601 ggctgatagt ttatttgtag gaataattat gttgatatta ggattggttc ttttgtctgt
      661 tggaatggta ttttatatgg gaagtggttt aggaagtgga cctagagatg gtttgatggt
      721 tgcaattcaa aagaaaacag gaaaatcact taaattaata agaggtacta tagaagttgg
      781 agcattaata gtaggatttt tattaggtgg aaaagttggt ataggaacta taataagtgc
      841 atttggattg gggtatttca cacaaatggt atttggctta tttaaattag attgtagtaa
      901 gattaaacat agatttatag tagatgatat aaaatttatc aaagtgtata tgagtggtga
      961 gaaaaaggct gctgttcaaa atatagttaa gtaatttaag aataaattca acgaaatgtt
     1021 aagtatttta aattaacatt ttgtttagat agataaattg aatattaaaa gaatattaaa
     1081 atatgaggat tgctagaaca ctataaaata tagatttcta gcaatttttt atttgttcaa
     1141 tattttttta gtactagaag tcttattctc tatttaatga atgaattaat ttataattag
     1201 agatactaaa ctttatagat attaatataa acacctttaa actgtgtatt ttccaaagcc
     1261 tttaaaatgc ttgtatttat tctaaataaa aaaataaaaa aatgtcaaaa aaactcttgt
     1321 attatatcca aattttatat ataattacct atgttgttta gaaattttaa actcaatgtt
     1381 tagtaaacaa aatgtttagt aaaataaaac agaaactagg aggtgcaaca tggatatagg
     1441 tgaaaagata aaaagattaa gaacagaaaa acaactgacc caggaagagt tagctaatag
     1501 atgtgaattg tcaaagggat ttatttctca attagaaaat aacttaacat caccatccat
     1561 agctaccctt atagatatac ttgaaatact aggtacaaat ttaagggaat tttttaatga
     1621 aatagatgat gaaagaatca gttttacaaa agaagatatg tttgaaactg aagatgaaga
     1681 tttaaaatat aaattaaagt ggttaattcc aaattcacaa aaaaatgaaa tggagccaat
     1741 aataataaca ttgtatccag gaggacagta taaagaagaa aagccacatg aaggtgaaga
     1801 atttggatat gtattagcag gttcaatata tgtccatata ggcgaaaaga aaaataaagt
     1861 aaaaaaagga gagagttttt actttagacc gaaagcaaat cactatatat caaacgaggg
     1921 caaaactaca gcaaaagtga tttgggtaag tacgccacca tcattttaga atagaggtgt
     1981 ttaataattg gtagaaaata taatagaatt aagggatata tctaaacatt atgaagaact
     2041 tactgtttta gataatttca atttagatat aaagaaaaat gagtttctaa cattacttgg
     2101 accaagtgga tgtggaaaaa ctacaacatt aaaaataata gcaggatttg agtatgctga
     2161 tgatggtaaa gttttatttg aaggtaagga gataaataat ttgccaccat atgaaagaca
     2221 agtaaacaca gtatttcaaa aatatgcttt atttccacac atggatgttt atgaaaatgt
     2281 agcttttgga ttaaaaatta aaaagactcc aaaagatgta atagatgcta aagtaaaaga
     2341 ggttttgaaa cttgttgcac tagaaggttt tgaaagacga caagttgaat cattgagtgg
     2401 aggacaacaa caaagaattg caatagcaag agctctagtt aatgaaccaa aggtattatt
     2461 actagatgaa ccacttggag cacttgattt aaagttaaga caagagatgc aaatagaatt
     2521 aaagagaatc caaaaaaaat taggaataac atttgttttt gtaactcatg accaagaaga
     2581 ggctcttact atgtcagata ctattgttgt aatgtataag ggcaaaattc aacaaatggg
     2641 aacaccacaa gatatataca atgaacctaa aaattcattt gtagctaagt ttataggtga
     2701 aagtaacata tttgatggaa ttatgataga agattataaa gttaatttct gtaatagaga
     2761 ttttgagtgt gttgataaag gattcgaaaa aaacgaaaat atagaagtag taataagacc
     2821 agaagatata aaaatggtaa agccagaaga aggaatgtta aaggggaaag ttacatctac
     2881 agtgtttaaa ggagtgcatt acgagataga attgaatgaa aatggaagat tgtggatact
     2941 tcataatact aaaaatgctg aggttgggac tgaactaggc atggatatat atccagaaga
     3001 tatccatata atgagaaaag agaataacta agatgaaaag aaaatctttt ttagcatatc
     3061 catatgtagt ttggagtgcg atatttgtaa taattccttt aatattagta gtatttttta
     3121 gttttacaaa agaatcaggt ggaggttatg cctttacact tgagaattat aaagaagtaa
     3181 ttgaccctat atatatgaaa gtttttgggc gttctattct tttagcagga ggagctacat
     3241 taatatgcct tatagttggt tatccagtgg catatataat ttcaaaggct cgtgtaagta
     3301 gaagaggttc attaatatta ttgtttattc ttcctatgtg gatgaacttt cttttaagaa
     3361 catatgcatg ggttgctata ttaggaaaaa atggactttt aaatacattt ttaggttggt
     3421 ttggaataca accattagca attttgtata ctaattttgc catactttta ggtatggtgt
     3481 ataacttttt accatttatg gttcttccaa tatatacagc actatcaaag atggataatg
     3541 atttgataaa cgctgctcat gacttgggtg caaataatat gacagttttt agaaaaatta
     3601 tatttccttt gagtttgcca ggtgtaatgt ctggaataac tatggtattt atgccagcag
     3661 tcactacttt tgcaatatca agactgttag gtggaggtaa aataatgctg gttggagatt
     3721 tgattgaaca acagtttaca gttgttggag attggaactt tggttcagct atatcgatat
     3781 ttatgatgat agtaatatta atatctatgt ctataatgtc aaaatttgga gatgaatctg
     3841 acaaagaagg cggtgggtta ctattttaaa attaaagaaa ataacttcta atatatattt
     3901 agcattagta ttcttatttt tgtatgcacc catatttgct ttggtactat tttctttcaa
     3961 tgattcaaaa tcaatggcaa gatggaatgg atttacatgg aagtggtatg gtcaattact
     4021 tcaaaatgag agtataatga gtgctttata ttatactatt gtaatagcta tattagcatc
     4081 tgtaatatct acaatagtag gaactataag tgctatagga atacataaga tgagaggtaa
     4141 gagtaagaaa ttaatactaa atgtaaacta tcttcctatt ttaaatacag agatagttac
     4201 agcagtagct cttatgagtc tgtttgtatt tgtaaaaatg gaatttggat ttacaacaat
     4261 gctattggca cacataatgt tttgtttgcc atacgttata ctttcagttt tgccaaaaat
     4321 gaagcaatta cctgataata tagaagatgc agctatggac ttgggagcaa cacctatata
     4381 cgctcttaga aaagtaattt taccacaaat aaaaccaggt atagtttctg gatttttaat
     4441 tgcatttact atgtcaatag atgactttat aataagtttc tttaatgctg gaaatggtgt
     4501 aagcaatctt tctatagaaa tctatggaat ggctagaaga ggtataaaac ctgaaataaa
     4561 tgcattatca acaataatgt ttgcagtggt tctaggttta cttttactag ctaataaaaa
     4621 agaatctata gtaaggggga ttaaataatg attaagttta aaaaagcaat atcccttata
     4681 gctgtaggta tgatgatagt tactttattt acaggatgta ataaagcaac tgattctaca
     4741 aaagttttaa atgtatataa tgttggagat tatatagatg agtctcttat agataagttt
     4801 gaaaaagaaa caggaataga tgttcaatat tcaacttatg atactaatga aatgatgtat
     4861 caaaaagtaa agagtggtag tacacactat gatttagtat ttccttcaga ctatatggta
     4921 gagaaaatga aaaatgaagg tcttttggaa aaattagatt ttaagaatat acctaatatg
     4981 aaatatttag ataagagttt tttaaatcct gtatacgaca aaactaatga atatagtgtt
     5041 gcatatatgt ggggaactct tggaatattg tacaataaga aagaagttaa agaccctatg
     5101 gatagctgga atatactttg gaatccaaag tataagggaa atataatgat gtttgactct
     5161 gttagagata ctatagggat aactcttaaa aaattaggat atagcatgaa tagtgtaaat
     5221 ccaaaagaaa taaatgaagc taaaaatctt ttaatgaaac aaaaggattt agtattggct
     5281 tatgtaaatg atgagggtaa ggatagatta cttggtggag aagtggcaat gggtatgctg
     5341 tattctggag atgcagttac actaatggag caaaatcctg accttgatta tgctatacct
     5401 aaagagggaa ctaataaatg ggtagatgct atgtgtgtac ctacaacagc acaaaataag
     5461 aaagaagctg agctgtttat aaacttttta ttagacccag agaatgcaaa agtaaatgct
     5521 gagtacattg ggtattcaac acctaataca ggagcactaa aattattaga cccagaaata
     5581 actcagaatc ctgttgcata tccatctaaa gaagtacttg ataaatgtga aacttttgaa
     5641 gatattggtg aaaatataaa attatatgac aaggcttgga tagagttaaa atcaaaatag
     5701 ggtcttgttg gtttaagtaa atttacttaa taaatactta ataaaactgt tcttttttag
     5761 aaaatttatt ttctaattat tgaacagttt tattttttgt ttaagttata ttcatatgca
     5821 atttaaatag tttattaata aatgaaaact tatttgtata atgactttaa taataattag
     5881 aaaaactggg caacttaata gaataattgg aggtgtaaat ttataaaaaa tgaataggat
     5941 aaataaaaaa atagatttat taatattggc tttgctcgtt tttatatttt taattgttgg
     6001 aatagtaata ctatcgatta ggacagaaaa taaattgaat gtgttcataa tgttagcaat
     6061 aatttttttt ataataatgt taacttattt atcaaattca gtagtggggt tgataacaag
     6121 ttcaataatt atatttatgt atacatccta tattttatat aacaatatta ctcacaatat
     6181 ggatgttgaa tttatatcat atatgtggat aatagcaaca ccagtatctt caattataat
     6241 gggtaatctg aacaaaagca taaatgaatt gcaaaataca aataaaaaac tatctgaaca
     6301 gtataaggaa ttagtgacta ttgatagtga aactggactt agaaatttaa agatttttta
     6361 taatgatgta aatatggaga taagtaaatc tattagacat aatactgatt tttctcttat
     6421 gatagtaaaa ttaccatact acggaaacct acagactata tttggtgaaa acaaaactaa
     6481 taaaatagta aaatatattg gttcaaatat tatagaatgt acaagaaatg aagatataat
     6541 ttatagttta caaaaagata tgatagggat actaatgcca aatacatctt tggaaggttc
     6601 aaaagttgtt aaagacagaa taaagaaaag gataaaagaa ttaaacttag atttaaacaa
     6661 taggggaaaa tatgtaaata tagatgtcaa aattgcattt ttacaatata agagtagttt
     6721 tggagatagt ataaatttta agaatatagt tgaagaggaa cttcaatatg atgtttaaaa
     6781 tatttaataa aagtctatta aaattagtag ttgtattatc aatagtactg acaatatcaa
     6841 attttagctt atcttcatac gcatcagaaa ttacttccag cacagataag gtattaatca
     6901 tatatgattc taagaaagaa acagcttata atagagatat tttaaatatt atgaggactc
     6961 tattgggaag atttagttca gatatagagc tattaaaatt gagtaactat gatggtgaga
     7021 taaataaaaa ttattacagt catatattta ttttaggaat caatgaaaac tcatataata
     7081 atgataaaaa cactaaaaac ttgattagtt ccttaaattc atataaagga actatatgtt
     7141 ggttaggata tggtatagaa aatcttttgg aacataaaaa atacaattta gactatgtag
     7201 gaaaaacaaa caatatagtt agtgtaaatt atagaggtaa atcatataac ttggatgaac
     7261 attatgtttt taacattgta gaatcaaaag atacaagtaa taaagtaata ggaagtatta
     7321 atgatacctt aaataagtat ccatatataa taaatgataa aaatcttttt tatgtatcaa
     7381 aattagattt agatggagtt ttattttata tattttgcga ttcacttaat gatattttta
     7441 acattaagac gtttgataaa ggacgtatat ttgtaagaat agaagatgta catgctttta
     7501 gagaaccaaa aaatcttgta gagatagcgg attatttaag tagtaagaat ataccattta
     7561 cgattgcatt aataccagca tatgtaaatc ctaaaaatca taaagtaata actttatcag
     7621 aaagccctga aattgtcaaa gcaataaaat atatgcaaga taaaggtgga actgtgatat
     7681 tgcatggata tactcatcaa tacaagaaag aagaagtttc tggagaaggg tatgaatttt
     7741 gggatggtaa aaaagatgaa cccttaaaag aaaatatgaa gatatttgtc aaagatagag
     7801 ttttgaatgg tcttagagta tgtatagaga atggaattta tcctcttgca tttgaagcgc
     7861 cacattatgc aatggaatca gagggatata aagaattaaa aaaatatttt tctacttata
     7921 tggggcaaca tcaaaataat gataaaaaat ttagcacaaa tacatatcct tatattatta
     7981 gggatacaga ggagtttaat atatttatac ccgaaaattt aggatatata gacccagaag
     8041 ataagtttac ttttcaaaat ataaaagaaa atttagataa attatctatt gtaagaggtt
     8101 ttagtggagg gttcttcttt cattcatatt taaacataga gtatctaaaa aacacaatag
     8161 aatatttaga aaaacagaat atagaattta tgaatttaag agattttaat aattgggtaa
     8221 aagttgatga aatacaaata agaaataatg gagatgaaat tatagtcaac tatgataagg
     8281 atttagatga aataactaaa agtgatacta gatttaaatc catatccaat attagtaaaa
     8341 ttttaatatt catagtatct ataagtgttt taatatttgt gattatattt atatatttta
     8401 agagaataga taaaaagaaa tttttaaaat aaatgatttt atattagaat caatatttta
     8461 aaatatttta ggagaaattt atgagagatt taaatgtatc agagtatttg ttcatattta
     8521 gtctgtttag tatatggtct ttattgctta taaatattat tttagctatg ggtggatata
     8581 tattttattt taaaaacttt gataaagaaa taaaagaaat tgatgaatat cctatgatat
     8641 caatacttgt tcctgctcat aatgaagcta aagtgatagg tagaactgtg gagtctttgc
     8701 tattactcaa ttatcctaag agtaagatgg aacttattgt tataaatgac aattcttctg
     8761 ataatagtaa agaaatttta gaaaatataa aggataggta caacaattat aatttcacta
     8821 taataaatac agatagttta acaggtggaa aaggaaagtc taatgcatta aatattggat
     8881 atacgatttc aaaaggagat tttatagctg tttatgatgc agataacaca ccagataaaa
     8941 atgcacttag atatttagta caaactatag taatgaatga tgaattaggt gctgttattg
     9001 ggaaatttag gactcgaaat aaaaataaaa acctattaac caaatttata aacatagaaa
     9061 cattaagctt tcaatggatg tcacaagctg gtagatggca actttttaat ctatgtacaa
     9121 taccaggaac taactttata ttgaggagaa gtatcataga agaaattgga ggatgggata
     9181 gtaaagcaat tgcagaagat acagaaataa gttttaggat atataaattg ggatacaaga
     9241 taaagcttgt accacaatct ataacatggg aacaagagcc agaaactgtt aaggtgtgga
     9301 taaagcaacg tactagatgg gcaaaaggga atatatatgt attaatgaaa tatataaaaa
     9361 atatatttaa acagggaaga aataagattg tgtttgatat agcttacttt ttttctgtat
     9421 attttctatt tttaacatct gtaattatat ccgatatttt atttgtttta agtatatcta
     9481 aactagtaga aatttctatt ccaattaact tttttctaat atggatacta tcttatcttt
     9541 tatttattat agaagtgagt ataagcctta ctatagaaaa aggtgaagca acaattgaaa
     9601 atatattcat agttgcaata atgtatttta catactctca actatggctg tttgtagcta
     9661 taaaaggaat gatagagtat ctaaaagata taatttttaa aagagaggtt aaatggtata
     9721 aaacagaaag attttgattc atgtacagga ggaattaaca tgaaaaaatt tattatatca
     9781 attatatctt tagtattgtt tttttcaaat atatcactta tatacaaagt aaatgctgat
     9841 gagactaaag ttaaaaatta taagtttgaa agagatataa ctattgatgg tgttatagga
     9901 agtaatagta ctttttttga agtaaataaa aattgggata ttgaagaagt tttattgcat
     9961 cttaatttta gtaagagcca aatattaaat ggagatgttt caagtttaac tgtattgata
    10021 aataatgtac caataaaatc tattaaatta aatgctaaga caaattataa aaatacatta
    10081 gaagtattag ttccaaaaga ttatataatt caaggatata atgaaataaa aataaaaaca
    10141 tacaaaacaa tatctgataa aatttgccaa gatgattcca atactgggaa ctggatggtt
    10201 attcacaaag aatcatatat atcaattaga tataagcaga aaaaggtgga aaactcaata
    10261 aatgaatatc cgtatccata tgcagaaatt gaaaataatc ataaattaga tacaactata
    10321 gtagtaccag ataatatgac tagaggagag actacagcag tgtttaattt ggcatcagca
    10381 tttggaaaaa taacaaaaaa tgatgattta aagttagatg ttaaattata ttcagaaatg
    10441 aaaaattggt cagatgataa tattatatat attggtaaac ctgaaaatac agcagaagaa
    10501 attttggata ttttaagtat aaaggaacaa accctattat cttcaaactg tataatcaag
    10561 caagtagatt caccatacaa taagaataag aagatgatgg tggtaattgg tagtaatgaa
    10621 gatgatttaa taaaggcatc aaatttatta atagaaaata gactttctaa ccaagtttta
    10681 tcatcatcag tacttgtaaa caaagagact aatataaaaa ttaataggga acaaaaatta
    10741 aatttaggtc atttaacatt aaaagactta ggatattctg actttttgtt ggaaggagca
    10801 tttaatcaac aagctttgtt tgatgtcaaa ataccaacgg gtaaggtatt agatgatggt
    10861 tcaaagatta tcttaaattt aagatattct gacaatttag attttgaaaa atctttagtt
    10921 acagtgtcta taaatgatgt tattgtggga agtaaaaaat tggataggtc acattctaat
    10981 aatgataagt tagaattaaa aatacctaaa gatatagata ataaaaacta ttatcaagtt
    11041 aaattgacat ttaatttgag tataaaaaat tcgaattgtg ttactagaga gagtaataat
    11101 ccttgggcat atgtatcaaa taattcttat ttggcgttat ctacgaaaga aaatgaaacc
    11161 ttatcttttg aaaattatcc gtatccattt gttagagatg atgaatttaa tgatttaact
    11221 gtgataatgc cagattattc agggtcacaa gctatgacct ggatgtttag attaggtgtt
    11281 actttaggtg cgaatataaa ttcacataat ggaaatataa atgtaattag aggaaaagaa
    11341 tttagtgaca agtataagga tactaatatt gtagtttttg gagtgccaca taataattct
    11401 gttataaaga tgttaaataa taatttaaat ataaagttcg ataaaaatta ctccaatttt
    11461 atatcaaatg ataagataag ctttattgat gattatggaa aaaatatttc taccattcaa
    11521 ttaataaaat caccatataa taatcaaaaa aatatcatgg taattagttc aatgaatgaa
    11581 aaaaacttat atttaggaat ggattatttg ttgaataaaa gtaaagttaa tgatttaaaa
    11641 ggagatacat tgataattga tgaatacgga gaggtagaag atttagctta taatttaaaa
    11701 tctaagaaag aagtaaaaga ttcaagttgg aatatgagta taaataagac aactaaagta
    11761 tttttaatga tttcttttat tactataatt gtagtcatga tattatcaat gctatatata
    11821 aaaaaatata aaagaagata atatgataaa tttattattt taaaagaggt atataatttg
    11881 aggaaatata tgaataaatt taaattaaaa agaagtattt taatctcatt aataatctta
    11941 atttttatat ttatagttta ctttgtaagt attgagttaa aagataggag tctagtaaat
    12001 aatcaatata aaggtaagat taaatcggcc aatttatctg tagattatga aataaatcaa
    12061 gttatgaagg atatagataa attgggattg aatacagtaa atgtacctat aattataaat
    12121 gtagagtcca ttaattctga tattatgtca atagataata atagtaaaga aaaggcaatt
    12181 aagttaataa aaaaattaaa taaaaaaggt atttcaacaa tattagaggc atatccatgg
    12241 attgaaaatg gaaaattgta tgagactgac tggaacccta taaataaaaa aagatttttt
    12301 tatacttggc aaaatgccat attaaatgaa cttataattg atgtagcaaa tccactggat
    12361 gtaaatgttt taaatattgg ttctaatttt gtacatttgg aagaatatca acaaaattgg
    12421 ggtgaaataa tagattttgt acaatccaaa tttgacggtc ttgtaacgta cagaactaat
    12481 tggtggtata caaaaaaaga tgatttaagt agtaagttgt tttatgaaca aaagttgaac
    12541 aataattttt ttgataagtt agattttata tctatagcag catattttga gttaagtaat
    12601 aaacctgtta atacagtgga tgaattaatt tctgccttac actcatctac tgtaaacaat
    12661 agaggacaaa atataaaaca agaaatatat aacctttata aagctcatag aaaacctata
    12721 ttttttggag aattaggttt ttcaaataga gaaagtgcaa gttctcagcc gtggaatcat
    12781 actccttcaa aagctgtaaa tggcgaagaa caagcaaggt gttttgaggc atataagaaa
    12841 gtttttgaaa atgaagactg gataaatggt ttttctgtgt tttgtgtagg taaaatagat
    12901 gacgaaaaaa atttttaccc atcaaaagag agtataaaag ttattaagtc ttggtatgaa
    12961 taattttgaa agtgtagaat ttatatagat taaatagatg attaggagag atataaatga
    13021 atgacatcaa ggttatgact gtatttggaa ctagacctga agctatcaaa gtagcacctt
    13081 taataaagga acttgaaaaa agagagaata taaaaagtat tgtttgcgtt acagctcaac
    13141 atagagaaat gcttgaccaa gttatagaaa cctttaatat aaatgtagac tatgatttgg
    13201 atataatgga aaaaggacaa agcctgaatg atataacatg taagatttta aataaacttc
    13261 cgttgatttt aaacaaggaa aatccaaata taatattggt acatggagat acaaccacaa
    13321 ctctagctac aagtttgact gcattttata ataaaactct tgttggtcat atagaagctg
    13381 ggcttagaac ctatgataaa tattctcctt ttccagagga gttaaataga caacttacag
    13441 gtattattgc tgatatgcac tttgctccta ctaatttggc tagaaaaaac ttgatttctg
    13501 aaggaaaacc taataataat atatttgtaa ctggtaatac tgctattgat gctttaaaga
    13561 tgacaataaa agaaaactat aatcatccta taattgatga gattggtaac gataggatga
    13621 ttttgttaac ctctcacaga agagaaaatt tgggtaaacc tatgaaaaat atattcagag
    13681 ctataaagcg aatagtagat gactttgaag atgtacagat agtatatccc atacatttaa
    13741 atcctaaaat acgaactata gctgatgaga tatttggaaa atttcctgaa aaaattcata
    13801 ttatagagcc tttagatgta gctgattttc ataacttttt aaataaatct tatatgataa
    13861 tgactgatag tggaggaata caggaagaag ctccatcatt aggtaagcca gttcttgtgt
    13921 tgcgtgataa aacagaaaga acagaaggaa ttgaagcaaa gacacttaaa ttagtaggta
    13981 ctaatgaaga tagaatatat aattcagtga gcgatttatt aataaataaa gataactatg
    14041 ttcagatgag taaagcatct aatccatatg gagatggaaa tgcaagcaaa tatatagtgg
    14101 atataataat taaaaaattt aattgcaaat atttaaatta gataaggcct aaaacattaa
    14161 ctaattgata taagatttat gataatatta aggaatacaa tttttaaggg ggagtaatat
    14221 ggctagaaaa ttaataaaaa atttgggtaa aagtaaaagt gtaaagagag tgaaactttt
    14281 atttaagaag atttttatta ctgtattcat agtagcaagt atagttgcta tttttaatat
    14341 aacgaaatat tttgaggaat tatacaaagt aagggattta aaaagtacta aaattgaata
    14401 ctatatggat gtggctgatg aagcaggaga tggaaaggtc caattaagtt ggaaggcctt
    14461 acttgctata gacatggtaa tacatgatga agatttaagt aatataaaaa agaaagacac
    14521 attggatata ggggaaaagt ttatagtaga agataaaaat ggtaaaggcg aaaaggtgta
    14581 taaggtaaaa aagtttaata aggtattaag cgaattgaaa tttgactctt ctcaaaaaag
    14641 tagagcaaga aaatacatga aagatttaga atacacatac cttggaaata aacaattaga
    14701 tagtagtgat gaaaaaatta aatttataaa gaagttagaa gactcagcta taagagaata
    14761 tattgattat ggaatattgc cctctataac aattggacaa gctatattag aatctggttg
    14821 gggaaattct aaacttacaa aacagagtaa taatttattt ggtataaaag cagataaagc
    14881 atggaaagga aaaagtgtag aaatttcaac ttcagagcat tataatgaaa aaattgtagc
    14941 tagttttagg tcgtataatt cattacaaga ttctgtcaaa gatcacagtt tatttttaat
    15001 taataataaa agatatagaa aacatgggtt gtttgaggca aaagattata ttagtcaagc
    15061 tcaagcatta gaaaatgcag gatatagtac agcagaagat aaaaaaggaa atcgtatata
    15121 tgcagagcta ttaatagatg ttattaggag ttataatttg caattgatag ataataaagt
    15181 tgagacaaaa taattgttaa ctttatatga ggagataaca agataagatt tttttctaca
    15241 aaaagaatag aaaaattgta aaaaaaacta aaaaaatgta ttgacttgtt atttaaaagg
    15301 tgatattatt attactgtac tcgaaagaga acaaaa
//
LOCUS       DBGJFF010000090        14989 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.091, whole genome shotgun sequence.
ACCESSION   DBGJFF010000090 DBGJFF010000000
VERSION     DBGJFF010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14989
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.091"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            177..833
                     /locus_tag="ACOIAF_002704"
     CDS             177..833
                     /locus_tag="ACOIAF_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669708.1"
                     /translation="MKKGDFIWSGVLICIIAVLAIPSSREVFMEATGAHPYIGGFLKF
                     AVLATMGDLLGARILYKDWKIPVGVFYRAIVWGIIGVVTTYGMSIYSSGVGQAQVDDM
                     LPFEGIAFANALFKSATMNILMSPTVFLFHKVMDCFIDKKYEVGKGIKVKLKDVVNIV
                     DWNAYLGFSVLKTIPFFWIPCHTIVFLFPAEYRVIASAFASIALGLILALANKSKVAV
                     "
     gene            complement(1341..2612)
                     /gene="sleC"
                     /locus_tag="ACOIAF_002705"
     CDS             complement(1341..2612)
                     /gene="sleC"
                     /locus_tag="ACOIAF_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex-lytic germination protein SleC"
                     /protein_id="HGZ2669709.1"
                     /translation="MQDGFLTVSIIDATNNRPIQNAVVNIYSMSNGSQSSSTLYQNLR
                     SNESGQVTGLVLPAPDVDYSLQPSDVRPYSQYIVEAIADGYETVVIEGTQLLATIEAR
                     QGVPMSPRTRSKRSFSRQSELIFDIGEHTLYGTYPPKIPESNLKPLPPPTGFVVLDNP
                     VVPEFIVVHDGLPEDSSAPNYWIPFKEYIKNIASSEIYSTWPEQTIYANVIAIISFTL
                     NRVFTEWYRNKGYNFTITSTTAYDHKFINNRNLFEPINVVVDAIFNTFIKRPPTSRQP
                     LLAQYCDGQKSQCPDQMTQWGSKDLGDQGYDYESILRYFYGDEIVFERAPIVSGVPVS
                     FPGTTLQVGSSGQYVRTIQNQLNAISNSYPAVPKVIEDGIYGTDTENAVKIFQGIFGL
                     PQSGVVDFKTWYEISRVYVATTRIASLNPLI"
     gene            2857..3573
                     /locus_tag="ACOIAF_002706"
     CDS             2857..3573
                     /locus_tag="ACOIAF_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HGZ2669710.1"
                     /translation="MKLDLFLLAIVPILIGMFWIRSKDRYCREPLIHLIKFFLIGAFL
                     SVIIILLENLLMKFNVFEGYSELIYVSFVVAGLVEEGVKALILIPALIKEKHFTEKLD
                     GIIYSVFLALGFATIENMVYIFSESRNLALQVGINRAVISIPAHVMFAITMGYYISKY
                     KFEGNKNKRREYLFMAVLIPILLHGVFDFILMIEYRWAIILLIVYVIILWKINLDKLE
                     KYMNHSKKVFFGNLRKKKKK"
     gene            3652..4962
                     /locus_tag="ACOIAF_002707"
     CDS             3652..4962
                     /locus_tag="ACOIAF_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HGZ2669711.1"
                     /translation="MTELPKEFLEDIEEILKDEYDDFIKSYEESKTTGLRINTLKIDK
                     KKLFDLELFDLNQIPWAEEGFYYDDKIDRPGRNPLHEAGAYYLQEPSAMSVIPKADIK
                     EGEKVLDMCAAPGGKSTYILSKLNDTGLLVSNEINASRIKALGENLERFGARNCIITN
                     TDSNSLRKTFNGYFDKVIIDAPCSGQGMFRKDEVAISDWSYAKVVECQSIQKEIIRDG
                     YKMLKKDGILIYSTCTFSREENEHVINEFISEYPHAELIEMERLWPHKIKGEGHFVAK
                     IKKLEEEECNVKELKIKNLDKEIKDYREFEKKFLKVDFYSNKKNKFYLRGENLYLLPE
                     IYPDTKKLKVLRYGLHLGVLKKNRFEPSHALSHYLKVEDVKNVENFSVQSESILKYLK
                     GDVVNSNDSRGWVLVSVEGIPLGWGKESSGVIKNHYPKGLRKVF"
     gene            5062..5598
                     /gene="lepB"
                     /locus_tag="ACOIAF_002708"
     CDS             5062..5598
                     /gene="lepB"
                     /locus_tag="ACOIAF_002708"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HGZ2669712.1"
                     /translation="MGEAVKKEVVEWIKVIVIALVLAFAITRFIVPTIVKGESMYPTL
                     VERDYLIVNRIAYKVGEPKYKDIIVFKTDLTEENGKKKDLVKRVIGVPGDHVKIQDSK
                     VYVNDKLLDETSYIHNNRTDGDIDIVVPEGKLFAMGDNREKSLDSRYDEVGLVDDHTI
                     LGKVLVRLYPFSKIGTID"
     gene            5764..6300
                     /gene="lepB"
                     /locus_tag="ACOIAF_002709"
     CDS             5764..6300
                     /gene="lepB"
                     /locus_tag="ACOIAF_002709"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HGZ2669713.1"
                     /translation="MNETIKEEIVEWIKIIITALFFAFIITRFIKPTLVNGESMYPTL
                     KSHDYLVANRMTYKLSEPKCGDIMIFKTDLLQENGRKKELVKRVIGVPGDHLKIKDSK
                     VYINGKLLNEVSYIHDNYTEGDIDMVIPKGKVFAMGDNREVSLDSRYKEVGLVDEENI
                     KGKVILRVFPFTDIGIFE"
     gene            6310..7065
                     /locus_tag="ACOIAF_002710"
     CDS             6310..7065
                     /locus_tag="ACOIAF_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HGZ2669714.1"
                     /translation="MAVGIASLLFNIEEALNICESIKQITHLEIGIDNISECSELCKY
                     KERISKLGLSIGIHLPMELNTCENIEYIRNSWINFIEKIEFELKGFDLRYFNLHLGYV
                     MTNRVRKNRDKYLGNSVDFLDKLNTNSYVCIENTYSKGGDFSNIGNISYDFEYIFKRI
                     KNSKICFCYDTGHCLIDEDAYVKNLKDKIRLIHLSDNDGINDTHVGIGRGILSEEGIK
                     EVLTLDAEYLVLEINYEDIEDTISKLNNIVGEG"
     gene            7139..8806
                     /locus_tag="ACOIAF_002711"
     CDS             7139..8806
                     /locus_tag="ACOIAF_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HGZ2669715.1"
                     /translation="MKKINLKIEGKDKEYSLEENSPGIRLGDIAKEFCDEHKGYITLA
                     VVDNKLKELNCRVKKDCEINFLDTTNEDGERVYFRVMSFIFVMACREIFWDSRVTIEH
                     SLSDGLYCEVHIDRKLKEADVENIKNKMKEIVNNDYVIEKIEVTRNEAISIFENNEMY
                     EKAELLKYKEYDSAKVYKCRNYIDHFYGYMLPSTGYIKSFDIKLYNGGVIILGPSEED
                     KTLPMKFVPQPKLSSVYYEAEEWSRLMGIDKVVSLNKIIENNEYGDIIRTFEALHEKK
                     LSQIADMIKDGNKRVVLIAAPSSSGKTSFAHRLSIHLRVNNLNPISISLDDYFIDRKH
                     TPLDEYGNYDFESIYAIDLEKFNLDLKKLLAGEEIDDIRFNFKEGKREYTGKKIKIDS
                     NQPIILEGIHGLNPILTSSIPDEDKFKIYISALTQINLDDHNRIPTADLRMIRRIVRD
                     YNFRGYSADNTILQWASVRRGEKKNIFPYQEEADAIFNSACVYELAVLKRFAKPLLEE
                     IKEDNPAHIEATRLLKFLQYFVELDDTSDIPGISILREFIGGSKIVD"
     gene            9490..10419
                     /gene="glsA"
                     /locus_tag="ACOIAF_002712"
     CDS             9490..10419
                     /gene="glsA"
                     /locus_tag="ACOIAF_002712"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HGZ2669716.1"
                     /translation="MNTLDETLLKEIISSNKKYTNYGQVASYIPELKNARRNDLGICI
                     IDSENNLYSAGNCSTKFTIQSISKPIVLAMALMDNDWEDVFSNVGMEPSGDPFNSIMK
                     LEINDTKKPCNPMINAGAIVTTSLINGSCLEEKEERMLSFFRKLAKNDNIGINYDVYK
                     SEKMTGDRNRAMAYLLKSDGFIRGNVEDVLDLYFKQCSIEIDSVDLARIGINLANYGV
                     DIENGEHLMSEMVSRIVKTFMMTCGMYDASGEFAIKVGIPAKSGVGGGIMASVPGRMG
                     IGVYGPALDKKGNSVAGVKVLEELSNKLKLNIF"
     gene            10517..11080
                     /locus_tag="ACOIAF_002713"
     CDS             10517..11080
                     /locus_tag="ACOIAF_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGZ2669717.1"
                     /translation="MKEVLVLRDLECIKAIAHPRRIDILKAFDKSPLSAKQLSQLLEE
                     PHAKINYHIKMLYKVGILELVEEKIKSGIVEKYYYPSAKNVVIGNRILNFSLDNGEEK
                     EELYISKFENMSEVFYKAAEEDVLENENIVDYHDISLTHDELVELSDTMKSKIDEILN
                     KRQHNVEGSKYDIAMVIVPTLEEECPS"
     gene            11567..12403
                     /locus_tag="ACOIAF_002714"
     CDS             11567..12403
                     /locus_tag="ACOIAF_002714"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008833 - deoxyribonuclease IV
                     (phage-T4-induced) activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HGZ2669718.1"
                     /translation="MIKLGTHLSIAKGFANAAETAVYIGANTFQFFSRNPRGGNAKEF
                     DEKDIIKFQEIRKENNFAPLLAHAPYTMNLGGTKDDVYEFATRVIDEDIRRMDSLGIE
                     YMCFHPGSHVGGGVDFGIDRIVKGLNNAIKGDENITILLETMSGKGTEIGFKFEQLKS
                     IIDKIEHKEKIGVCLDTCHIFSAGYDIVNDLDGVLEEFDKVIGIERLKTVHLNDSMMP
                     FGDKKDRHAPIGEGKIGLKALIDFMEHPSLKHLPFFLETPFDDEGHKRELKMIKEILS
                     SK"
     gene            12702..13697
                     /locus_tag="ACOIAF_002715"
     CDS             12702..13697
                     /locus_tag="ACOIAF_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HGZ2669719.1"
                     /translation="MRNLGNTNMKIKRVGFGGIPIQRITQDDTNLVINELEKQGINFI
                     DSARGYTISEEAIGIAIEGKRDKFFLATKSMSRDYDSMKRDVEISLNNFKTDFIDLYQ
                     FHNVKEEEYDNLFKDKMAYSALLEAKEQGKIKHIGITSHNLNTIEKAIEDGKFDTIQF
                     PYNIVEGQADEVFKKAHEKGIGIIVMKPLAGGALDNATLAIKYILSKDYIDVVIPGME
                     SVEQVRQNVAVLENLVLDEKDNKEIEEIRNSLGKKFCRRCEYCMPCAVGINIPLSFLC
                     EGYYTRYGLKEWAKEKYEVMDVKPTECIDCGLCESRCPYELPIREMLKTVVEKLG"
     gene            complement(13954..14358)
                     /locus_tag="ACOIAF_002716"
     CDS             complement(13954..14358)
                     /locus_tag="ACOIAF_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HGZ2669720.1"
                     /translation="MIKITIPGRPISKSNFKLHNINGQAWMPSKGKYSKYLAYENMIA
                     GFINQQYQGETVEENLITVLKLFFPNKRMGDLHNYPKSICDGIEKSGIIKNDKQLKPV
                     LLFDFIDKDNPRVEIELYPVSKYDISYNIFEK"
BASE COUNT         5719 a         1492 c         2758 g         5020 t
ORIGIN      
        1 gataatggtg gatagagtag taagaaaata aatagataaa gggatagaat aaatttaaat
       61 atggaaaatg aatatctaat aataagtgat taataaataa ttgtagatag attgtaaatt
      121 aaataaatct ttaatagaaa ggagaactat ttatatatta aatagtattt ataaatatga
      181 aaaaaggcga ttttatatgg agtggagttc ttatttgtat tattgctgtc ttagcaatac
      241 caagtagtag agaagtattt atggaagcaa caggtgcaca tccatatatt ggaggttttt
      301 taaagtttgc tgtattagct acaatgggag atttacttgg tgctagaatt ttgtataagg
      361 attggaaaat accagtagga gtattttata gagcaatagt ttggggaata ataggtgtag
      421 ttaccacata tggaatgtca atctactcat caggtgtagg tcaagcacaa gtggatgata
      481 tgcttccatt tgagggaata gcttttgcaa atgctctatt taagagtgca actatgaata
      541 tactaatgtc accaactgta tttttattcc ataaggttat ggactgtttt atagataaaa
      601 aatatgaggt tggaaagggg attaaggtaa aacttaaaga tgtggtaaat attgtggact
      661 ggaatgctta ccttggtttt agtgttttaa aaactatacc atttttctgg ataccatgtc
      721 atacaattgt atttttattc cctgctgagt atagagtaat tgcttctgca tttgcatcta
      781 tagctttagg tctaatattg gctttagcta ataagtctaa agttgcagtt taggttcact
      841 gatgaagata gtagtattat ttaaattagg aataactttt taaattaata ttatttgtat
      901 ttataataaa taataattta tataagaagt aatattctaa gtgatagata gtgttttaaa
      961 taataagaag taatactcta agtgatagac agtgttttaa ataataagga gtaatactct
     1021 aagtgagaag tagaatttta aataataagt gagattttaa ataaaaagta ggtttttcgt
     1081 tagaatagca ttttattaaa cttaaaaata aatataatat tttaaagaaa tctaagttgc
     1141 taagcactgt ttttaagaag cttaaaatcc cccatttaaa gttttaagat tctaaaaaaa
     1201 caagttataa ctaaaaaatg gctgttaaga ctacaatact tataagtaat acttatggta
     1261 tatattagtt cataacagcc atttttattt taaattaata attttattca ataatttgag
     1321 tttatgttct aaatcaagct ttaaattaaa ggatttaaag aagctattct agttgtagct
     1381 acataaactc tggatatttc ataccatgtt ttaaaatcta ctactccgct ttgaggtaaa
     1441 ccaaagattc cttggaatat cttaacagcg ttttctgtat ctgttccata gataccatcc
     1501 tctataactt ttggtacagc tggataagaa ttagatatag catttaactg attttgtatg
     1561 gttctaacat actgcccaga agaaccaact tgtagtgtag taccagggaa tgacactggt
     1621 acaccactta ctataggtgc tctttcaaat actatctcat ctccatagaa atatcttagt
     1681 atactctcat agtcatatcc ttggtcacct aagtctttac tgccccattg agtcatttgg
     1741 tcaggacatt gggatttttg tccatcacaa tattgtgcta atagtggctg tcttgatgta
     1801 ggaggtcttt ttataaaagt attaaaaata gcatctacaa ctacgttaat tggttcaaat
     1861 aagtttctat tatttataaa cttatggtcg tatgctgttg ttgaagttat tgtaaagtta
     1921 tatcctttat ttctatacca ttcagtaaag actctattta aagtaaatga tattatagct
     1981 attacatttg cataaattgt ctgttcaggc caagttgaat atatttcaga tgacgctatg
     2041 tttttaatgt actctttaaa tggaatccaa tagtttggag ctgatgaatc ttctggcaaa
     2101 ccatcatgaa ctactataaa ttcaggcact acagggttat ctaatacaac gaaaccagta
     2161 ggaggtggta atggttttaa gttactttct ggaatcttag gaggataagt accatataga
     2221 gtatgttctc ctatgtcaaa aatcaactca ctttgtcttg agaaagaacg cttacttctt
     2281 gttctaggag acatgggaac tccttgtctt gcttcaattg tagctaacaa ctgtgttcct
     2341 tctatgacaa ctgtctcata tccatctgct atggcttcca ctatatactg gctataaggt
     2401 ctaacatcag atggttgtag agagtaatct acatctggtg ctggtaatac taaaccagtc
     2461 acttgaccag attcattact tcttaggttt tggtataaag ttgatgagct ttggctacca
     2521 ttagacattg aatatatgtt tactactgca ttttgaatag gtctattgtt agtagcatca
     2581 attatgctta ctgttaagaa accatcttgc atcaaatcac cctttcttta aatgaattta
     2641 gttcccttat atttcaagat atgaatatag cttaaaattt gttaaataat atagatataa
     2701 atttaaattt ttttgagtat ttgagtatat ttgtatttaa ataatttaaa ataatttaat
     2761 gtatatttta gtatgtattt aaaacacgaa taagttaaaa ataagtagat gatataaatc
     2821 ataaataaat caaaaatttt gaaaggaaaa taatctatga agctagattt atttttatta
     2881 gcaatagttc ctatattgat aggaatgttt tggataaggt ctaaagatag atattgtaga
     2941 gaacccctaa tccatctaat taaatttttt ttaataggtg cttttctaag tgttataatt
     3001 atacttttag aaaatttact gatgaagttt aatgtatttg aagggtattc agaactaata
     3061 tatgtatcat ttgtagttgc aggacttgta gaagaaggtg taaaagctct gatactgatt
     3121 cctgccctaa ttaaagaaaa acactttact gaaaagttag atggtattat ttactctgta
     3181 tttttagctt taggatttgc aacaattgaa aatatggttt atattttttc tgaaagtaga
     3241 aatctagcac tgcaagttgg tattaatagg gcagttatct ctataccagc tcatgtaatg
     3301 tttgcaatta ctatgggcta ctatatttct aagtataaat ttgagggaaa taaaaataaa
     3361 agacgtgagt atttatttat ggcagtgtta attccaattt tattacatgg agtttttgat
     3421 ttcatattga tgatagaata tagatgggct attatattac ttatagttta tgtaataatt
     3481 ttgtggaaga taaatcttga taaacttgag aaatatatga atcactctaa aaaagtgttt
     3541 ttcggtaatc ttaggaaaaa gaagaagaaa taaaatttaa aggaaagaca taaataatta
     3601 aagtataata aatgacacac aagtaaagca gattgaaggg ggattataga tttgacagaa
     3661 ttaccaaagg aatttttaga agatattgaa gaaatactaa aagatgaata tgatgatttt
     3721 ataaaaagtt atgaggaaag taagactact ggccttagaa taaatacttt aaaaattgac
     3781 aagaaaaaat tatttgattt ggagttgttt gatttgaatc aaataccatg ggcagaagaa
     3841 ggtttttatt atgatgataa aattgacaga cctggtagaa atccactgca tgaggctgga
     3901 gcatactatt tgcaagagcc ttctgcaatg agtgtaatac caaaggcaga tattaaagag
     3961 ggtgaaaaag ttctcgatat gtgtgctgct cctggtggaa aatctacata tatactatct
     4021 aagctaaacg atacaggttt attggtttct aatgaaatta atgctagtag aattaaggct
     4081 ctaggggaaa atttagagcg atttggagcg agaaattgta ttataacaaa caccgattct
     4141 aatagcttaa gaaagacgtt taatgggtat tttgataagg ttattataga tgcaccttgt
     4201 tctgggcaag gtatgtttag gaaggatgag gttgccatat cagattggag ttatgcaaag
     4261 gtagtagaat gtcaatccat ccaaaaagag attataagag atggatataa aatgttaaag
     4321 aaggatggca ttttaattta ctcaacttgt actttttcaa gggaggaaaa tgagcatgtt
     4381 ataaatgagt ttatcagtga atatccacat gcagaactta ttgaaatgga aagattatgg
     4441 cctcataaga taaagggaga aggtcatttt gttgccaaaa taaaaaaatt agaagaagaa
     4501 gaatgtaatg tcaaagaatt aaaaataaaa aatttagata aagaaataaa ggattatagg
     4561 gagtttgaaa agaaattttt aaaggttgat ttttatagta ataaaaaaaa taaattttat
     4621 ttaagaggag aaaatttgta cctacttcct gaaatatatc cagatacgaa aaaactaaaa
     4681 gtattgagat atggtttgca tttaggggtt ttaaagaaga atagatttga gccttctcat
     4741 gcactatctc attacttaaa agttgaagat gtaaaaaatg ttgaaaactt cagtgtacag
     4801 agtgaatcaa ttcttaaata tttaaaaggg gatgttgtaa attctaatga tagtagagga
     4861 tgggttttgg tttcggttga aggaatacct ttaggttggg gaaaagaatc ttctggtgta
     4921 attaagaatc attatcctaa aggtcttaga aaagtatttt aaactattag aaaaaagaag
     4981 tataatattt atattgtagg tttatggtag attattttgc tgtagattga cttaatattt
     5041 aacacagagg agacgatttt agtgggtgaa gcagttaaaa aagaagttgt agaatggata
     5101 aaagtgattg tcatagctct tgttttggca tttgcaataa ctcgttttat agtgccaaca
     5161 atagtcaaag gagaatcaat gtatcctaca ttagttgaac gtgattattt gatagttaac
     5221 agaattgcgt acaaggtagg agagccaaaa tacaaagata taatagtatt caaaaccgac
     5281 ttaacagagg aaaatggaaa gaaaaaagat ttagtaaaaa gagttatcgg ggttcctggt
     5341 gaccatgtaa aaatacaaga ctccaaggta tatgtaaatg ataagttgtt agatgagact
     5401 tcctatatac ataataatcg tactgatgga gatattgata tcgtagttcc agaaggaaaa
     5461 ttatttgcaa tgggagataa tagagaaaaa agtttagata gtagatacga tgaggttgga
     5521 ttggtcgacg atcataccat tttaggaaag gttctagtca gattgtatcc attttctaag
     5581 ataggaacta ttgactaaac atataaaatt tggagcaatc gctgattagc tgaaatcacc
     5641 agaccataca caatagttgt ctatataaaa aagacaacac cttatttagt agacttatct
     5701 aaaagatagt gtttaagaga cattatcaat aaagataata taactataaa ggagaatttg
     5761 tgaatgaatg aaactattaa agaagagatt gtagagtgga taaaaataat tattactgca
     5821 cttttttttg catttattat aactcgtttt ataaaaccaa cattagtaaa tggagaatca
     5881 atgtacccaa cacttaaatc acatgattat ttggtagcaa acaggatgac atataagtta
     5941 tcagaaccaa aatgtggaga tataatgata tttaagactg atttattaca agagaatgga
     6001 aggaaaaaag agcttgtaaa aagggttata ggtgttcctg gtgaccatct aaaaattaag
     6061 gattctaagg tttatataaa tggtaagtta ttaaatgaag tttcatatat acatgataat
     6121 tatactgaag gcgatattga tatggtgatt cctaagggaa aagtatttgc gatgggagac
     6181 aatagagaag ttagtttaga cagtagatat aaagaagtgg gattagtaga tgaagaaaat
     6241 attaaaggaa aagttatttt aagagtattt ccttttacag atataggtat ttttgagtag
     6301 gtgatttgta tggcagttgg tatagcgtcc ttattgttta atattgagga ggctttaaac
     6361 atttgtgaga gtataaagca aataactcat ttagagattg gaattgacaa tatatctgaa
     6421 tgtagtgagt tatgtaaata taaagagaga atttctaagt taggattatc tatagggata
     6481 cacttgccta tggaattaaa tacttgcgaa aacatagagt atatacgaaa ttcatggata
     6541 aattttattg agaagataga atttgaacta aaaggatttg atttaaggta ttttaacctt
     6601 catttgggat atgttatgac aaatagagtt agaaagaata gagacaaata tttaggaaat
     6661 agtgtggatt ttttagataa attaaataca aattcatatg tatgtataga gaatacttat
     6721 tcaaaaggtg gggatttttc aaatattgga aatatatctt atgattttga atatatattt
     6781 aagaggatta aaaattctaa gatttgtttt tgttacgata ctggacactg tttaattgat
     6841 gaggatgctt atgtaaagaa cttaaaggat aaaataagac tgatacactt aagtgataat
     6901 gatggtatca atgatacaca tgttggtata ggaagaggaa ttttatctga agaggggatt
     6961 aaagaggtct taacattaga tgcagaatat ttagtattag aaataaatta cgaagacatt
     7021 gaagatacta taagtaaatt gaataatatt gtaggagagg gttaaatacc tctctttatc
     7081 ttgttgatat gcctcattaa ttaaaattta tattcatcaa aaggaaggag atgttgaagt
     7141 gaaaaaaata aacttaaaaa tagaaggaaa agataaggaa tattcattag aagaaaattc
     7201 ccctggaatc agattgggag atatagcaaa agaattttgt gatgagcata agggttatat
     7261 aacacttgca gttgtagaca ataaactaaa agagctaaat tgtagagtta agaaggattg
     7321 tgagataaat tttttagata ctactaatga agatggagag agagtttact ttagagtcat
     7381 gtcatttata tttgtaatgg catgtagaga aatcttttgg gatagcagag ttacaataga
     7441 acattcacta tcagatgggc tatattgtga agttcatata gatagaaagc ttaaagaagc
     7501 agatgttgaa aatataaaaa acaagatgaa agagatagta aacaacgatt atgttataga
     7561 aaaaattgaa gttacaagaa atgaagctat ttctatcttt gaaaataatg aaatgtatga
     7621 aaaggcagag cttttgaaat acaaagagta tgatagtgct aaagtatata agtgtagaaa
     7681 ctatatagac catttttatg gttatatgtt gccatcaact ggttatataa aatcatttga
     7741 tattaagctt tataatggag gcgttataat tttaggacct agtgaagaag ataagactct
     7801 tcctatgaaa tttgtacctc aacctaagct atcaagtgta tattatgaag cagaagaatg
     7861 gtcaagactt atgggaattg acaaggtagt atctcttaat aaaataatag aaaacaatga
     7921 atatggagat ataataagga catttgaagc attgcatgaa aagaagttat cccaaatagc
     7981 ggatatgata aaggatggca ataagagagt agttttaata gcagctccat cttcatctgg
     8041 aaaaacaagt tttgctcaca gattatcaat acatcttaga gtaaataatt taaatcctat
     8101 ttcaatttct ttagatgatt attttataga tagaaaacat actcctttag atgaatatgg
     8161 aaattatgat tttgaatcaa tttatgcaat agacttagag aagtttaact tagacttaaa
     8221 gaaactttta gctggtgaag aaattgatga tataagattc aattttaaag aaggtaagag
     8281 agaatacact ggtaaaaaga taaagataga ttcaaatcag ccaattatat tagaaggtat
     8341 acatgggcta aatcctattt taacatcatc tataccagat gaagataaat ttaagatata
     8401 cataagtgcc cttactcaaa taaatttgga tgaccataat cgaatcccta ctgctgattt
     8461 gagaatgata agaagaattg ttagagacta taattttaga ggatatagtg ctgataatac
     8521 tattttacaa tgggcatcag ttagaagagg agaaaagaag aatatattcc cgtaccaaga
     8581 ggaggcagat gctatattta actctgcttg tgtatatgaa ttggcagttt taaaacgatt
     8641 tgcgaaacct ttgttggaag agataaagga agataatcct gcacatattg aagctactag
     8701 attactaaag tttttgcagt attttgttga attggacgat acttctgaca taccaggtat
     8761 ttcaattctt agggaattta ttggaggaag taaaatagtt gattaatgtg tgtattatag
     8821 attgttttat gaagattaaa tggcatagca agtagtgtga ttggtttatg ttaagtatat
     8881 taagtgtaaa gtggagaatt aaattgatac agattttaaa gtttgagttg atatataaaa
     8941 aggcttgttt tatacttttg tatgaagcaa gctttttatt ttaaatttta cttttttgtt
     9001 taatattttg ttagtttatg tgattatttt ttaaaagagt aaaagagtaa aagagtaaaa
     9061 gagtaaaaga gtaaaagagt aaaagagtaa aaggtactgg gttagaaatt tttggtatta
     9121 taaaaattta cattactgga ttactagatt gttaaattgt tgaattatta ggttgttgta
     9181 ttactaaatt actaggttat taagttactg gattactaga ttgctaaatt attagcttgt
     9241 taaactattg aattattaga ctattggatt gctaaattat taggttgtta aagtattaat
     9301 tattagattg ttgtattact aaattactag attattaaat tattaagcca tttaaactat
     9361 ttaaattatt tgaaaaatga attgattctt gtagaaaata attttaaagt ataatttttt
     9421 ttaacactgt tttattatag tataaacttg tgctaaaata gaatatataa cataatataa
     9481 gaggtgcata tgaatacgtt agatgagaca ttattaaaag aaattataag ttctaataag
     9541 aagtatacta attatgggca agtcgctagt tacataccag aattaaaaaa cgctagaaga
     9601 aatgatttag gaatttgtat catagatagc gaaaataatc tttatagtgc tggaaactgt
     9661 agcacaaagt ttactataca aagtatttca aaaccaattg tgcttgctat ggcacttatg
     9721 gataatgatt gggaagacgt tttttcaaac gttggaatgg agccaagtgg ggacccattt
     9781 aattcaataa tgaaacttga aattaatgac acaaaaaaac cctgtaatcc aatgataaat
     9841 gcaggggcta tagtaactac ttcccttata aatgggagtt gtttagaaga aaaagaagaa
     9901 agaatgctta gtttctttag aaagttagca aaaaatgata atataggaat aaactatgat
     9961 gtctataagt ctgaaaaaat gactggagat agaaacagag ctatggctta cttattgaaa
    10021 agtgatggat ttatcagagg gaatgtagaa gatgttctag acctttattt taaacaatgc
    10081 tcaattgaga tagattctgt agatttagcg agaattggaa taaatcttgc aaattatgga
    10141 gtagatatag aaaatggtga gcatttaatg agtgaaatgg tttctaggat tgtcaagaca
    10201 tttatgatga cttgtggaat gtatgatgct tctggggaat ttgctattaa agttggcatt
    10261 cctgcaaaat ctggagttgg gggtggtata atggcttctg tgccgggacg catggggatt
    10321 ggtgtttatg gacctgcgct tgacaaaaaa ggtaacagcg tcgcaggagt taaagtttta
    10381 gaggagttgt caaataaact caaattaaat attttttaaa atattaagaa aggatttttt
    10441 tgacaacaac atataattta atatataatt aaattgtaaa gaaatatgaa ttaaataaat
    10501 tttttaggag gggtcaatga aagaggtatt ggtattacga gatttggagt gcataaaggc
    10561 tattgcgcat ccacgtagaa tagatattct aaaagcattt gacaaatcgc cattgtcagc
    10621 aaaacaatta tctcaattat tggaagaacc tcatgcaaaa ataaactacc atataaaaat
    10681 gttatacaaa gttggtattt tagaattagt tgaggaaaaa ataaagtcgg ggattgtgga
    10741 aaagtactat tatccaagtg caaaaaatgt ggttataggg aataggatac ttaatttttc
    10801 tttagacaat ggggaagaaa aagaagaatt gtacatatca aaatttgaaa atatgagtga
    10861 agttttttat aaggcagcag aggaagatgt tttagaaaat gaaaatatag ttgattatca
    10921 tgatatttct cttactcatg atgaattagt tgaacttagt gacactatga agtcaaagat
    10981 agatgaaatt ttaaataaaa gacaacacaa tgttgaaggt tctaagtatg atatagctat
    11041 ggttatagtt cctactcttg aggaggaatg tcctagttag agaattaatt atggattaat
    11101 taaagatata atattagaga ttgaacatca aaatttactt aataatcaaa aaatagtttt
    11161 tggtatgggt aaaacaatga tttttaatct ctattttttt gttttttata cctattgtat
    11221 ttgtaaataa tatgtatagt gtaagatgta tacactatct aagaagtgct tagataatat
    11281 acaaagtatg taagattata atatttgaaa ttgattaaaa tgggtataat taaagtgtaa
    11341 atattaaaat taaaaattaa tataaattaa atcaatgcta gaaatatgtt aaaagaggac
    11401 tgagattagt tgggatagaa catcccttag agcttacgaa tttttagtga aaaagtacta
    11461 tcaaatagga atctccatct tcatatgttg gctaaatgta gttaaggtgg agtagttcaa
    11521 aattaaaatt gagtacaagt agtagtacaa ataaaaggag attaatatga ttaaattagg
    11581 aacacatctt tcaatagcaa aaggatttgc taatgcggct gagacagctg tatatattgg
    11641 tgcaaataca tttcaatttt ttagtagaaa tccaagagga ggaaatgcta aagaatttga
    11701 tgaaaaagat ataattaaat ttcaagaaat tagaaaagaa aataattttg caccactgct
    11761 tgcacatgca ccatatacta tgaatctggg tggtacaaag gatgatgtat acgaatttgc
    11821 aacaagggtg atagatgagg atataagaag gatggactca ttaggaattg aatacatgtg
    11881 ttttcatcct ggaagtcatg taggtggtgg tgtagatttt ggcatagata gaatagtaaa
    11941 aggattgaac aatgccataa agggtgatga aaatataact atacttcttg aaactatgtc
    12001 gggaaaggga actgaaatag gttttaaatt tgagcaatta aaaagtataa tagacaaaat
    12061 tgagcataaa gaaaaaatag gtgtgtgtct tgatacatgt catatttttt cagctggtta
    12121 tgatatagtg aatgatttag atggggtttt ggaagaattt gataaggtta taggcataga
    12181 aagattgaaa actgtccatc taaatgatag tatgatgcca tttggagaca aaaaagacag
    12241 acatgctcct ataggggaag gaaagatagg attaaaagca ttaattgatt ttatggaaca
    12301 tcctagtcta aaacatttac catttttctt agaaactcct tttgatgatg aaggtcacaa
    12361 gagagagctt aaaatgataa aagaaatact atctagtaaa taaaccttta ttaaaaatat
    12421 gtgaaatact ccgtcctcaa cataattagg gtggagatat aatcatgaaa tgtgataatt
    12481 taaattaatg gataggaata acaaaaattg tatacaaatt aactgggtat aaagtatgat
    12541 tatataaagt atgattatat tatgaataga ttgatataaa atataattat attatgaata
    12601 gtatgtaaat atattgcaaa gattctcata atttgttgta taattaaagg tgacatattt
    12661 atattatata aaaaattatt aaaagttagg tggggaaaga tatgagaaat ttaggcaata
    12721 ctaacatgaa aataaagaga gttggctttg ggggcattcc aatacaaagg attactcaag
    12781 atgatactaa cttagttata aatgagttag aaaaacaagg aataaacttt attgacagtg
    12841 caagagggta tacaataagt gaggaagcaa tcggaattgc cattgaagga aaaagagata
    12901 aatttttctt agctactaaa agtatgtcca gagattatga ttcaatgaag agagatgttg
    12961 aaataagttt aaataatttt aaaactgatt ttatagattt atatcaattc cataatgtta
    13021 aagaagagga gtatgataat ctttttaaag ataaaatggc ttatagtgcc cttttagagg
    13081 caaaggagca aggtaagata aaacatattg gtataactag tcacaattta aatactatag
    13141 aaaaagctat agaagatgga aaatttgaca ctatacaatt cccttacaat atagttgaag
    13201 gacaagcaga tgaagtgttt aaaaaagctc atgaaaaagg aattgggata atagtaatga
    13261 aacctttagc tggaggtgct ttagacaatg caacactggc tattaaatat atactatcta
    13321 aagattacat agatgtagtt ataccaggta tggagagtgt tgaacaggtt agacaaaatg
    13381 tcgctgtact tgaaaatctt gtgctagatg aaaaagataa taaggaaatt gaagaaataa
    13441 gaaattcttt aggtaaaaaa ttctgtagaa gatgcgaata ttgtatgcct tgtgctgtag
    13501 gaataaatat accattgagt tttttatgtg aaggttatta tactagatat ggattaaaag
    13561 agtgggctaa agaaaaatat gaggttatgg atgttaaacc tactgagtgt attgactgtg
    13621 ggctttgcga gagtagatgt ccatatgaac ttcctataag agaaatgctt aagactgttg
    13681 ttgaaaagct aggatagatt ttatttattg aaatcggtgt attaaattta ctccgatttc
    13741 tttttattag atgtggtctt atatacaaat attaaattta catagttata gctattatat
    13801 aaatttaata tttgtttaat acttattaaa tatttactta atgcttttta aaaacatttg
    13861 tttgagtata gacataacac aattattatt gttgtattag tatagtttca aatactggag
    13921 tatcaatata atgtcgaata acacataaaa tctttatttt tcaaaaatat tataggatat
    13981 atcgtattta gatactggat aaagttcaat ttcaacccta gggttatctt tatctataaa
    14041 atcaaataaa agtactggct taagttgttt atcatttttt attattccac ttttttcaat
    14101 accatcacaa atactttttg gataattatg taaatccccc attctcttat ttgggaagaa
    14161 taatttcaag actgttatta gattttcttc aacagtttcc ccttgatact gttggtttat
    14221 aaaacctgca atcatgtttt cgtatgcaag atacttagaa tattttccct ttgaaggcat
    14281 ccatgcttga ccatttatgt tatgaagctt aaaattagat ttactaatag gtctacctgg
    14341 aattgttatt ttaatcaaat ttttcaccat ccttatacag tatttattat aatctgtata
    14401 tgatttaaaa gcaaattgtt atttaaagtg tttttacaga gcttgtttgt aaaattttcc
    14461 actttagatg tagtaatata agataagtat tttgcttaca aattttaaaa tttgatatat
    14521 gatgggcata ttcatattag actattataa gaccttcata atattaataa gtataagttt
    14581 agtaaagaga gtaaaaagaa atccatataa gtcaagtaaa gacttaataa atggcaatgt
    14641 aaatgggata ttaattataa gaaaaaatca atctttttgc attaactata ggaagaatgt
    14701 caaaatataa ctaatgatat ttgaagtttt agagtttata ttaaaaagta agaaggttaa
    14761 gtgggtaaaa aaggttgaat agattaaaaa ggctaggtat ctaaaagagt tagtaggtaa
    14821 aaaagttaaa taagtaaatg aaaaaggcta agtatataaa ggagttagta gataaaaaaa
    14881 ttaaatagat aaaaagatta agtatctaaa agcgttagta gataaaaaaa ttaaataaat
    14941 aaaaaaaact aagtattcaa aagagttagt aggtaaaata attaaatag
//
LOCUS       DBGJFF010000091        14913 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.092, whole genome shotgun sequence.
ACCESSION   DBGJFF010000091 DBGJFF010000000
VERSION     DBGJFF010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14913
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.092"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            55..279
                     /locus_tag="ACOIAF_002717"
     CDS             55..279
                     /locus_tag="ACOIAF_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669721.1"
                     /translation="MIIKLTKDNFNFRFKQYEGMFFHAEVVEGMESIRYKITKDKEGT
                     ELKEFGSTHIKDNMIYVPQVEAYIDLNSIK"
     gene            332..856
                     /locus_tag="ACOIAF_002718"
     CDS             332..856
                     /locus_tag="ACOIAF_002718"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGZ2669722.1"
                     /translation="MSKPIDITIRPIRREDAQAFNEIRRTYSVMRNTLALMSDRVDKT
                     EAMLASLGENDYMFVAETNNNGEKDVIGFAGLHVNSSPRLRHSAEIGISVAENYQGRG
                     VGKKLMEQLLDIADNWIMLTRVGLEVIVDNEKGLNLYKKLGFEIEGTKKYAVIRDGKF
                     EDVYIMGRYNKNLI"
     gene            complement(961..1107)
                     /locus_tag="ACOIAF_002719"
     CDS             complement(961..1107)
                     /locus_tag="ACOIAF_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669723.1"
                     /translation="MDMDLLFKIVFAIVAIGVSVKIIKTVASLAFKIALILLIILFVY
                     RMFL"
     gene            complement(1246..2172)
                     /locus_tag="ACOIAF_002720"
     CDS             complement(1246..2172)
                     /locus_tag="ACOIAF_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HGZ2669724.1"
                     /translation="MNFSNIFIQVAVLFIIILVGYFVRKFNLLDDHCTSKLSTLTMTV
                     FLPSMIISSMQINFDSKMIQKILLLLFTSLIMYIVSIIIAFLLKYILKCNDKKDLGIY
                     QYIVVFSNVAFMGYPVIEAVLGHEAIFYTAIFNLPFNLFSFTLGIYLLSKGSTSRGFS
                     IKSLISPATIAIVIGLFLFVTGLRLPQFINEPLEMLGSITTPISMIIIGSLLANSSAL
                     DCFVNKKLYIVTFIRLLVLPVIVYFILKGWINDKMILAIPVVISSMPAAANTAIMANQ
                     YDSNITLASQCVFFTTLFSVISIPFISILLLS"
     gene            2362..4233
                     /locus_tag="ACOIAF_002721"
     CDS             2362..4233
                     /locus_tag="ACOIAF_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HGZ2669725.1"
                     /translation="MKNTKFSIKKIKNISNDPKVVHTKGALLEKMGKMESAIELYKSA
                     ALDNYVKSQYTLGNIYESKKQFKEAEKWYSMAYKSGSEDAAFDLGNMYYKLDGYEYAI
                     YWYEKIANLGYLQAQNNLGVCHFKMKDFVRCEKWLKTAADKNLGKACFNLGVLYTFLE
                     KDDEAYEYYKKGSVLLDDDAKYNLAILNGKKKDNKSTVSLYKQLYKSGHMKGCFNLGM
                     IMEINDNLEDAEKYYKKSADRDDVKSEYRLAYVYDRKEELGDAIYYYEKAIEKNHTLS
                     KYRLANLYNRENEIEKAKTYYKMAAEDDITEAKNNLAGIYFEEKDYENAIKYYEDAIA
                     VGCKSSLENLGDLYYQNQDIEKAISYYSRIPNNVSCQIKLGNIYEDLNNIEEAISWYK
                     KASENGDTRSSYRLGCIYESLGNTKNARKYFEMASSKNHMNARIHLGRIYFREGKLEE
                     SKMMFDTPANENNVYAQHMVGLIYDMFYKDYVNSKFWYEKARAQGCVESIYNLGQIYL
                     KLNDDAEAEKYYKEGIKYGSKKCEYMLAGLYYKKSLDMYISLANEEYDNCEEIVEGFP
                     NLNIDFDEVLIPPFKLQEEVIDEEEYVPIYILNIKESLDSMLEGLETEMIIDEEHDS"
     gene            complement(4325..5260)
                     /locus_tag="ACOIAF_002722"
     CDS             complement(4325..5260)
                     /locus_tag="ACOIAF_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HGZ2669726.1"
                     /translation="MIRYYKTIDSKLEKLSFFEDGCWINLVEPNHSEINEISNLLNID
                     VESIESALDEEERSRIDVEDNHTLILIDIPVDESDSNSSHYTTIPLGIILTEEAIVTV
                     CDAQTKILNDFIVGHIKDFFTFKKTRFLLQILHKNAAYYLHYLRKINKMTIIIEREIY
                     KSMKNKELVQLLELEKSLVYFSTSLKSNELVLNKMVRTAGIKKYPDDEDLLEDVIVEN
                     RQALDMAKIYGDILSRIMDAFSAIISNNQNNVMQILTVVTLIFSIPTIISGFFGMNVI
                     NMPFSNNPNGFWIILLITSIICIVITFFMSRNKLL"
     gene            5780..6292
                     /locus_tag="ACOIAF_002723"
     CDS             5780..6292
                     /locus_tag="ACOIAF_002723"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HGZ2669727.1"
                     /translation="MSDFKEILAEEIKNNPFGLIGKDWTLITAENEGKVNTMTASWGG
                     LGVMWGKDVAFVVIRPQRYTKEFIDNTDKFSLTFFDEDFRKELSYCGKVSGREEDKIS
                     QIGFNIEHLNDTPYFKESKMAIICKKMYSQKLEPQCFIAEGIDGRWYPQKDYHTLYIA
                     EITNVLVKED"
     gene            complement(6447..7502)
                     /locus_tag="ACOIAF_002724"
     CDS             complement(6447..7502)
                     /locus_tag="ACOIAF_002724"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent flavin oxidoreductase"
                     /protein_id="HGZ2669728.1"
                     /translation="MKELIIRDLVAKVPIIQGGMGVGISRSSLAGAVAKLGGVGVISG
                     VQIGYDEEDFETNTINANLRAIKKHISKAKEISNGGIIGINFMVAMKEYETYVKEAVK
                     AGVDLIISGAGLPNKLPSLVKGSNVKIAPIVSTAKAANVILKMWDRKEKTTADLIVVE
                     GPKAGGHLGYSNEELDNIDSIDYDKEFVEILKVANTYGEKFGRNIPVVAAGGITSSSD
                     VKKYIDMGASGVQVGTRFVATYECDAHENFKMAYINASEEDVHIVKSPVGLPGRAIRN
                     KFIEEVKINRPEIKKCYNCLIPCNPKETPYCISQALINAVKGDVENSLLFCGNDAYKI
                     DKLSTVEDVINELISEL"
     gene            complement(7632..8525)
                     /gene="thrB"
                     /locus_tag="ACOIAF_002725"
     CDS             complement(7632..8525)
                     /gene="thrB"
                     /locus_tag="ACOIAF_002725"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HGZ2669729.1"
                     /translation="MLEIIVPATSANIGPGFDCLGIALNIYNKFYVEEIESGLEIEGC
                     EDAYKNENNLVYTSMKYFFDRVKPEKIPAGIKIKIQSEVPICRGLGSSASCIVAGVIA
                     ANALSGANLDKNQLLNIASEIEGHPDNVAPAILGNMIVSVTDNENIHYDIIKIPEELK
                     FCAMIPNFKLSTEKARGVLPKEIPYSDGVFNVSRVALLISALLNKNFDLLKVACQDKL
                     HQDYRGTLIENYNDIVEKSEQLNSIGVFLSGAGPTIMSLIKENDDSFVDNMKNYLQKL
                     KSDWEIKELCCDSNGAVLNII"
     gene            complement(8535..10016)
                     /gene="thrC"
                     /locus_tag="ACOIAF_002726"
     CDS             complement(8535..10016)
                     /gene="thrC"
                     /locus_tag="ACOIAF_002726"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HGZ2669730.1"
                     /translation="MYYSSTRGTEEKVTASQAIIKGISNDGGLYVPSEFPNVKNELIN
                     LVNLTYSQIAFFVLSKFLCDFTEDEIKNCIENAYDEKFDCSSIAPLNKVNDTYFLELY
                     HGPTLAFKDMALTIMPHLLKTSIKKDNLEKDVVILTATSGDTGKAALEGFKDIDKIKI
                     IVFFPEDGVSPVQKLQMKTQTGKNTYVVGIKGNFDDAQSGVKKIFSDKEFNQKLLDNN
                     YILSSANSINIGRLLPQVAYYFYSYMKLVSNSDINLNDKINFVVPTGNFGNILAGYYA
                     KQMGLPINKLICASNDNNVLYDFFKTGVYDKNRTLKLTTSPSMDILISSNLERLLFEI
                     SNRNTDIVNKLLSDLSDKGVYKISDDMEKNLASFYGEYSNEEEVKSTINNVFKNYNYL
                     IDTHTAVAYNCYEKYKKETSDNTKTVIVSTASPFKFSEDVLKSIDCDFKDLDDFAIID
                     RLSSISKIDIPKPIKNLKNAEILHKDIYEKDELKLAIKKFLKV"
     misc_binding    complement(10124..10355)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            11157..12065
                     /gene="trxB"
                     /locus_tag="ACOIAF_002727"
     CDS             11157..12065
                     /gene="trxB"
                     /locus_tag="ACOIAF_002727"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HGZ2669731.1"
                     /translation="MVDIIVIGAGPAGLTSAIYAMRAGLSVTVFEKNIYGGQVASTSE
                     VENYPAVQKISGVEFSNNIYNQAVAQGVDIQFDEVEEINLEGKVKVVKTSSGEHKAKA
                     VILANGVERRKLGCAGEQEFTGRGVSYCATCDGAFFKDKEVAIVGGGNTALEDALFLA
                     NNCTKVYLIHRRDSFRGEEVLEKSVKARENIEILYSHGVEKIEGEKTVSKIEVKNLKT
                     EEKRTIDVSGIFIAIGLKPNNKMFENVLDLDEGGYIISDESCTTSVEGVYVAGDSRTK
                     FLRQIITAASDGAIAAVQAANYINVE"
     gene            complement(12155..13978)
                     /gene="typA"
                     /locus_tag="ACOIAF_002728"
     CDS             complement(12155..13978)
                     /gene="typA"
                     /locus_tag="ACOIAF_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HGZ2669732.1"
                     /translation="MSQKHKIINIAVIAHVDAGKSTLVDAFLSQSGVFRKNEVVKDCV
                     MDSNDLEKERGITIYSKNCAINYEDYKINIVDTPGHSDFSSEVERVMKTVDTVILLVD
                     ASEGPMPQTRFVLQKSLEFGLKPILFINKIDKKDQRAEEVVNEVFDLFVDLNATDEQC
                     EFPIIYGIAKQGIAKLEMDDDSEDLSPLFKTIVNHVEAYPNYDNEPLQFQISALAYDD
                     YVGRLGIGRIYKGTLKNNTQVAICREDSVVSKGKVSKLSVYEGLKQVEVDEATSGEIV
                     VIAGIPDISIGETICDLDSPLPMEMIKIEEPTLSMNFLVNDSPFVGKSGKFVTTRHLK
                     DRLEKELEVNVGLKVEPLDTTDGYKVSGRGELHLSILLENMRREGYEVGVSKPEVLMH
                     KEDGKLMEPIERVVVNCPEVYSGTIINELNMRKGMMESMSIEGDYVKIEFLAPTRGLL
                     GYRSEFINATRGEGTLVRSFEKFEEFKGEIPSRGNGVLIAQGPGVTMGYSLNALSDRA
                     VMFVDPGVEVYEGMIIGMNSRKDDMVVNPCKNKKMSNVRASGSDDAIKLSPPRIFTLE
                     EALEFIEDDELVEITPDSIRLRKRFLNEHDRLRYNKSRQGK"
     gene            14190..14432
                     /locus_tag="ACOIAF_002729"
     CDS             14190..14432
                     /locus_tag="ACOIAF_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669733.1"
                     /translation="MLEVTKIIIKYFASESDTFVKEEVCNFEELLKWSHKFIEGKKIE
                     DVLYIKSKENYKVNVIKSLEIKELEIVRKQQIPPYM"
     gene            14580..14771
                     /locus_tag="ACOIAF_002730"
     CDS             14580..14771
                     /locus_tag="ACOIAF_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1450 domain-containing protein"
                     /protein_id="HGZ2669734.1"
                     /translation="MIRVCPNCSNVDVNKLKTLVGEENVKTGCIGQCRAFKKEAVGII
                     DNELEIKETEAEFFEACKE"
BASE COUNT         5592 a         1919 c         2187 g         5215 t
ORIGIN      
        1 tatatacaac taataataag aaaaatatca attatataag gagtagataa aaatatgata
       61 ataaaactta ctaaagacaa ttttaatttt agatttaaac aatatgaagg tatgtttttt
      121 catgctgaag tagttgaagg tatggaatcc attagatata aaataactaa ggataaagaa
      181 ggtacagaat taaaagaatt tggaagtaca catataaaag ataatatgat atatgtacct
      241 caggtagaag catacataga tttgaacagt ataaaataaa gatgtaaaat tttaattaaa
      301 aataaagatt atgtttgagg gggaatccaa tatgagtaag cctatagata taacaataag
      361 acctataaga agagaagatg cacaagcttt taatgaaatt agaagaacat acagtgttat
      421 gagaaatact ttggcattaa tgagtgacag agtcgataaa actgaagcta tgttagcttc
      481 tttgggagaa aatgattata tgtttgtagc tgaaactaat aataatggag aaaaagatgt
      541 aataggtttt gctgggttac atgtaaattc ttctccacga ttgagacata gcgcagaaat
      601 aggaataagt gttgcagaga attatcaggg aagaggagta ggtaagaagc ttatggaaca
      661 acttttagat atagcagata attggattat gttaactaga gtaggattag aagtaattgt
      721 ggataatgaa aagggattaa atttatataa aaagctaggt tttgaaatag agggaactaa
      781 aaaatatgct gtaatcagag atggaaaatt tgaagatgtt tatattatgg gaagatataa
      841 taaaaattta atttaaataa gctaatagag aagtaaaata gagtgtcttt aataaaaaat
      901 ttacattaaa gataatctat tttacttctc tattgtgtta aaaaacttat ttttaaatgt
      961 ttacaaaaac attctatata caaataaaat tattagcaat atcaacgcta ttttaaaagc
     1021 taaactagca acagttttaa tgatttttac agaaacacct atagcaacta tagcaaatac
     1081 tattttaaag agtaaatcca tatccatatt taagcctcct attctataat acatatagat
     1141 attattgaca aatatctagg aaaaaattcc atcaatatac tacaataaaa tgtttatatt
     1201 tgtaatttat tgaatatgtt attatataat acataaaaaa ataaatcaag ataagagtaa
     1261 aatacttata aaaggtatag atataactga aaatagtgtt gtaaaaaaca cacattgtga
     1321 agctagtgtt atattggagt catattgatt agccattata gcagtatttg cagcagcagg
     1381 cattgaagaa attactactg gtatagctaa aatcatctta tcatttatcc atccttttag
     1441 aataaaatac acaatcacag gaagtaccaa taatcttata aaagtgacta tatataattt
     1501 tttgttgaca aaacaatcga gagctgaaga attagcaagt aagcttccaa tgataatcat
     1561 ggatattggt gtagttatac ttcccaacat ctcaagtggc tcgtttataa attggggtaa
     1621 cctaagtcct gttacaaata aaaaaagtcc aataacaata gctattgtag cagggcttat
     1681 aagtgatttt attgaaaaac ctctacttgt actgccttta gataaaagat aaattcctaa
     1741 agtgaaagaa aataagttaa aaggcaaatt gaatattgca gtatagaata ttgcttcatg
     1801 acctaaaact gcttcaatta caggataacc cataaaagca acatttgaaa aaacaactat
     1861 atactggtat attcccaaat cttttttatc attacatttt aaaatgtatt tcaataaaaa
     1921 tgcaattata attgaaacta tatacataat caaagatgta aataataaca atagtatttt
     1981 ttgtatcatt ttactatcaa agttaatttg catggagcta attatcattg atggtaaaaa
     2041 aactgtcatt gtcaaagttg aaagcttaga tgtgcagtgg tcatctaata agttgaattt
     2101 tctgacaaaa tatccaacta aaataattat aaataaaact gcaacttgga taaaaatatt
     2161 gctgaaattc ataaaatgtc ccccctatat atacattata taccaattac ataataaaat
     2221 ctaatgatta taaaatttaa aatttaatgt tacaatatag tgacaaaagg taaaaattca
     2281 atattatagc attaattgta gattataatg tataataatg tttgagagaa tatattttat
     2341 aaaatgggta ggtagcgaat aatgaaaaat acaaaatttt ctataaaaaa aataaaaaat
     2401 atatctaatg acccgaaagt cgtacatact aagggtgccc tccttgaaaa gatgggtaag
     2461 atggaaagcg caatagagtt gtataagagt gctgcattgg ataattacgt aaaatctcaa
     2521 tatactttag gaaatatata tgaaagtaaa aaacaattta aagaagcaga aaagtggtat
     2581 tcaatggcat ataaaagtgg aagtgaagat gctgcttttg atttaggtaa tatgtactat
     2641 aagttagatg gctatgaata tgctatatat tggtatgaaa agatagcaaa tttaggttat
     2701 ttacaagcac aaaataattt aggtgtatgc cattttaaaa tgaaagattt tgtaaggtgt
     2761 gaaaaatggt taaaaacagc agcagacaag aatttaggta aagcttgttt taatttaggg
     2821 gtattatata cttttttgga gaaagatgat gaagcatatg aatactataa aaaaggttct
     2881 gtgcttttag atgatgatgc aaaatataat ttagcaatat taaatggaaa aaagaaagat
     2941 aataaatcaa cagtaagttt atataaacag ttgtacaagt caggtcatat gaaaggttgc
     3001 tttaacttgg gtatgattat ggaaataaat gataatttag aagatgcaga gaagtattat
     3061 aaaaagagtg ctgatagaga tgatgtgaag tctgagtata ggcttgcata tgtatatgat
     3121 agaaaagaag agctgggaga tgcaatttat tattatgaga aagcaataga aaaaaatcat
     3181 acactttcta agtatagact tgctaattta tataatagag aaaatgagat agagaaggca
     3241 aaaacttatt ataaaatggc tgctgaagat gatataacag aagcaaaaaa taatctggca
     3301 ggtatatatt ttgaagaaaa agattatgaa aatgctatta aatattatga ggatgctata
     3361 gctgttggat gcaaaagttc ccttgaaaat ctaggggatt tatattatca gaatcaagat
     3421 atagaaaaag cgatttcgta ttattcgagg attccaaata atgtatcttg tcaaatcaaa
     3481 ttaggaaata tctatgaaga tttaaataat attgaagaag caataagttg gtataagaaa
     3541 gcatctgaaa atggagatac gaggtctagt tatagattgg ggtgtatata tgagagctta
     3601 ggaaatacaa aaaatgctag aaagtatttt gaaatggcaa gtagcaaaaa ccatatgaat
     3661 gctcgtatac atctaggtag aatttatttt agagaaggta aattggaaga gtctaaaatg
     3721 atgtttgata cacctgccaa tgaaaataat gtgtatgctc aacatatggt aggattaatt
     3781 tatgatatgt tctataaaga ttatgtaaat tctaaatttt ggtatgaaaa ggctagagca
     3841 caaggttgtg ttgaatcaat atataattta ggtcaaattt atttaaaatt aaatgatgac
     3901 gcagaagctg agaaatatta taaagaaggt attaaatatg gaagtaagaa gtgtgagtat
     3961 atgcttgcag gcctttatta taaaaagagc ctagatatgt acatttcttt agcaaatgaa
     4021 gagtacgata attgtgaaga gatagtagaa ggctttccaa atttaaatat agactttgat
     4081 gaagtattaa ttcctccatt taagttgcaa gaagaggtaa tagatgaaga agagtatgta
     4141 ccaatttata tactaaatat aaaagaaagt ttagattcaa tgctagaagg gcttgaaact
     4201 gaaatgatta tagatgaaga acatgatagt taaaaagtat tataagatta tatagattta
     4261 aatatataaa agccattagg ataaatgtaa atcaattaat tctaatggct tttttattaa
     4321 tattttataa tagcttattt cttgacatga aaaatgtaat cactatacat attatagagg
     4381 taataagaag tattatccaa aatccgtttg ggttattaga aaatggcatg ttgatgacat
     4441 tcataccaaa gaatcctgat attattgtag gtattgagaa aattagtgta actactgtta
     4501 aaatttgcat aacattattc tgattattac ttataattgc actgaaagca tccataattc
     4561 tactcaatat atctccatat attttagcca tatcaagagc ctgtctattt tcaactataa
     4621 catcttctaa taggtcttca tcatctggat acttctttat accagcagtt ctaaccattt
     4681 tgtttaaaac aagttcatta gattttagtg aggtagagaa gtataccaaa gatttttcta
     4741 attccaataa ttgaaccaat tctttgtttt tcattgattt gtaaatttct cgctctatta
     4801 tgattgtcat tttatttatt tttcttaagt aatgcaagta ataagctgca tttttgtgaa
     4861 gaatttgaag caaaaagcga gtctttttaa acgtaaaaaa atctttaata tgaccaacta
     4921 taaagtcatt taaaatctta gtttgtgcat cacaaactgt tacaatagct tcttcagtta
     4981 gtataatacc taagggtatt gtagtataat gagaagaatt agagtcactt tcgtctactg
     5041 gtatatctat aagtataaga gtatgattgt cttcaacatc tattcttgag cgttcttctt
     5101 catctagtgc agattctatg ctttctacat ctatatttaa taaattagaa atttcgttga
     5161 tttcactatg attaggttca acaagattta tccaacagcc atcttcaaaa aaactaagtt
     5221 tctctaattt tgaatctata gttttgtaat atcttatcat ggcaacacat cctttattca
     5281 gttttatata aatctcatca tttaatagta tctattaatt aactaattta tataactgaa
     5341 tatatgtatt agttttgatt ttagatatta ttaagtgatg aaaatgtaaa aaattctctc
     5401 cgcaacggac tagtatccat tgcccatcac tctccttatt tattatttta tatatagcta
     5461 tttatgaaat gcgtatttta agcactcttt aatataatac cttttccagg tatataaatc
     5521 aagttttgat ttgattaaat aatattttta ccataattta cacataaagt atataagtta
     5581 tatttaattt gagtaaagaa tatatatatt ctgagtgaaa ttattaaata tattaaaaaa
     5641 taagagagta tatatatatt tacttatttg caataatata tttaaaaaag tattttattt
     5701 tcaaaactag acttcattta taatttatca cgaaaataaa aggaatataa atataagaga
     5761 atgagaggtt attagagata tgagtgattt taaagagatt ttggcagaag agattaaaaa
     5821 taatccattt ggattgatag gtaaagattg gacacttata acagcagaaa atgaaggtaa
     5881 agtgaataca atgactgcta gctggggagg gcttggagtt atgtggggaa aagatgttgc
     5941 ttttgtagta ataagaccac agcgttatac taaggaattt atagataata cagataaatt
     6001 ttctcttaca ttttttgatg aagattttcg taaagagctt tcatattgtg gaaaggtatc
     6061 tggtagagaa gaggataaga tatcacagat tggatttaat atagaacact taaatgatac
     6121 accttatttt aaagaatcta aaatggctat tatatgcaaa aaaatgtata gccagaaact
     6181 tgaacctcaa tgttttattg ctgaaggtat agatggaaga tggtatccac aaaaagatta
     6241 tcatacttta tatatagctg aaataaccaa tgtacttgta aaagaagatt agttataaag
     6301 gttaatacta tctctaatta aattaaaatt tccataaaag gaaattgaca caaaaaagct
     6361 aaggacataa atatgtttat aaagattaat aaataacgta tttaaactcc ttagcttgat
     6421 aaaattttaa tttagttttc tttaaattat aattcagaaa tcaattcatt gataacatct
     6481 tcaacagtac ttaatttatc aattttatag gcatcatttc cacaaaataa aagtgaattt
     6541 tcaacatctc ctttaactgc attgattaga gcttgagaaa tacagtaagg agtttctttt
     6601 ggattacaag gaatcaaaca attatagcac ttctttattt ctggacgatt tattttaact
     6661 tcttctataa atttgtttct tatggcgcgt ccaggaagac ctacaggact tttaactata
     6721 tgaacatctt cttcactagc atttatgtat gccattttaa agttctcatg ggcatcacat
     6781 tcgtaagtag caacaaatct tgtaccaact tgtacaccac ttgcacccat atctatatat
     6841 tttttaacat cactactaga agttatacca ccagcagcaa ctactggtat gtttctacca
     6901 aatttttctc cataagtatt tgcaaccttt aaaatttcaa caaattcctt atcatagtct
     6961 atagaatcta tgttatcaag ttcctcgtta gagtatccaa ggtgaccacc agcttttgga
     7021 ccttcaacaa ctattaagtc agcagtagtt ttctcttttc tatcccacat ttttaaaatt
     7081 acattagcag cttttgcagt tgaaactatg ggtgctattt ttacattact tccctttact
     7141 aatgatggta gcttattagg taatccagct ccagatatta taaggtctac accagcttta
     7201 acagcttcct ttacgtatgt ttcatattct ttcatagcta ccataaaatt aataccaata
     7261 ataccaccat ttgagatttc tttggcctta gaaatatgtt tttttatagc tcttaaattt
     7321 gcatttatag tattagtttc aaaatcttcc tcatcatatc caatttgaac tccagaaatt
     7381 acaccaacac caccaagctt agcaactgca ccagctagtg atgacctaga tataccaacg
     7441 cccattccac cttgaattat aggaactttt gcaactaaat ctctaattat taactctttt
     7501 atgataatcc ctcctcaatg taaacatgtt taaagaacta gattataata cttaatatta
     7561 ttacctagtc ctaattatta tactacaaat tataataaaa gagtagttat attttgtata
     7621 gaagacgata attatattat atttaaaaca gctccattag aatcgcaaca taactctttt
     7681 atttcccaat cagattttag tttttgtagg taatttttca tattatccac aaaactatca
     7741 tcattttctt ttattaaaga cattatagta ggtccagctc cacttaaaaa tactccaata
     7801 ctatttaatt gttcagattt ttcaacaata tcattgtagt tctcaattag agttcctcta
     7861 taatcttgat gcaatttgtc ttgacaagct actttaagta ggtcaaagtt tttgtttagt
     7921 aaggcagata ttagtaaagc aactctacta acattgaata ccccatcaga atatggtatt
     7981 tcctttggta aaacacctct agctttttct gttgataact taaaattagg tatcattgca
     8041 caaaatttaa gttcttcagg aatttttata atatcataat gtatattctc attatcagta
     8101 acagatacaa tcatatttcc taaaatagca ggagcaacat tatctgggtg accttctatt
     8161 tctgaagcta tatttagtaa ttgattttta tctaaattag cacctgatag agcatttgca
     8221 gctataactc cagctactat acaagaggca ctactgccaa gacctctaca tataggtact
     8281 tcactttgga tttttatttt gataccagca ggtatttttt ctggtttaac tctatcaaaa
     8341 aagtacttca tagatgtata aactaagtta ttttcgtttt tgtaagcatc ttcacaacct
     8401 tctatttcaa gaccactttc aatttcttca acataaaatt tattgtatat atttaacgca
     8461 attccaagac aatcaaatcc tggacctata ttagcacttg ttgcaggaac tataatctct
     8521 aacataataa tcacctatac ctttaagaat ttttttatag ctagttttaa ttcatctttt
     8581 tcataaatat ctttatgtag tatttctgca tttttaagat ttttaatagg ttttggaata
     8641 tctattttag aaatacttga taatctatca attatagcaa agtcatcaag gtctttaaaa
     8701 tcacaatcta tagactttag aacatcttca gaaaacttga atggacttgc agtacttaca
     8761 ataacagttt ttgtgttatc agaagtttct tttttatatt tttcataaca attgtatgca
     8821 acagcagtat gagtatctat caaataatta taatttttaa acacattgtt tatcgtgctt
     8881 ttaacttctt cttcatttga atattctcca tagaatgagg ctaaattttt ttccatatca
     8941 tcacttattt tgtagactcc tttatcacta aggtctgata aaagcttatt tacaatatca
     9001 gtatttctat ttgatatttc aaacagtaat cgttctaaat tacttgaaat taatatatcc
     9061 atagaaggag aagtagttaa tttcaatgtt ctatttttat catatacacc agttttaaag
     9121 aaatcataaa gaacattatt atcatttgat gcacatatca atttgtttat tggaagaccc
     9181 atttgtttgg cataatatcc agctagaata ttaccaaaat taccagttgg gacaacaaaa
     9241 tttattttat catttaaatt tatatcacta ttgctaacta atttcatata tgaataaaaa
     9301 tagtatgcaa cttgtggaag aagtcttcct atattaattg aatttgcaga agataatata
     9361 taattattat ccaatagttt ttgattaaat tctttatcag aaaaaatctt ctttacacct
     9421 gattgtgcat catcaaaatt tccttttata ccaactacat atgtattttt tccagtttga
     9481 gttttcattt gaagtttttg tactgggctt acaccatctt ctgggaagaa tacaattatc
     9541 tttattttat ctatatcttt aaatccctca agtgcagctt tacctgtatc tccagatgta
     9601 gcagtaagaa tcacaacatc tttctctaaa ttatctttct ttatagatgt tttaagaaga
     9661 tgaggcatta tagtaagtgc catatcttta aatgctaaag ttggaccgtg atatagttct
     9721 aagaaataag tatcatttac tttatttaat ggagctatac tactacaatc aaacttttca
     9781 tcatatgcat tttctataca atttttaatt tcatcttcag taaaatcaca taaaaattta
     9841 ctaagtacaa aaaatgctat ctgagaatac gttaaattta ctaaatttat aagttcattt
     9901 tttacattgg gaaattcact tggaacataa agaccaccat catttgatat tccttttatt
     9961 attgcttgtg atgcagttac tttttcttcg gtgcctctcg tacttgaata atacattttt
    10021 tataatcctc catatatttt aaatttttaa ttagctaatt aaattagata cagaatattt
    10081 taaaaactga atttaggtaa taaaaaaaga ccctcatccc taaaaaaggg acgagaatct
    10141 gtttcccgcg gttccaccca aattgatttt aaaaaatcca cttgatactt taacgcaagt
    10201 aataaaaacg tctattccta ctaaaatcat taagataact tcggatagaa gctcctaggt
    10261 agttttcaat ccttctaatt agggaaacat ttcagctagt agtttccttc tctggtaatc
    10321 gattcagatt tactcttcct aatcaatgcc tttcaatatt cttaatatta ttaattattc
    10381 tagtttataa aacagacaaa gtcaagtatt aatataaaaa tttggaaaaa atatttttta
    10441 aattgtaaaa aaattgtaat caaattgagt attagaaaaa tattttttta acttttttaa
    10501 aagtttaaaa acctagattt tcatagtgat gaagctgttt ttaatgaaag taatcaaaag
    10561 ataactatta aatatctgta atattacaat tttatactac actatattac aaaaaaagtt
    10621 tacaaaactt taatttgaaa aatgtaatta tatggtatat catataaata taagtaaata
    10681 gtgctaatat ttaaactatt aatcataatt ggttcaacaa aagtatatag ttaataatgt
    10741 ttaatttgaa gaaattgtta gtgtgaaatt acatatatat tcaaattaaa ttcataaata
    10801 tttcacataa taatagtata ctgtaaacaa aaggaggtat taaatatggg aatagtactt
    10861 ataacagcag cattagtttt ggttatgtat ggactttcaa ttctatcata tatatcagaa
    10921 agcaatatat tagaagacac tgtaaatgat aaccaataag gttttgaatt atataaataa
    10981 gtgagaactg taaataaaag ttaattttta tttgcagttt ttttatatat atgtaaaata
    11041 aagttaatta ataaaaaata atgttttgtt tatagtatat atggataata atagtaaagt
    11101 ataagaatga tgagaataat atataagaaa taataatata aagagaggta aacgttatgg
    11161 tagatatcat tgtaattggt gctggaccag caggtttaac ttcagcaatt tatgctatga
    11221 gagcagggtt aagcgttaca gtatttgaaa aaaatattta tggaggtcaa gttgcaagta
    11281 cctctgaggt agaaaattat ccagcagttc aaaagatttc tggggtagaa ttttctaata
    11341 atatatacaa tcaagcagtt gctcaaggtg ttgatattca gtttgatgag gttgaagaga
    11401 taaatttaga gggaaaagta aaggttgtaa aaacttcttc aggtgaacat aaagcaaagg
    11461 ctgttattct tgcaaatgga gttgaaagac gtaaattagg ttgtgcaggg gaacaagaat
    11521 ttacaggtag aggagtatct tactgtgcaa catgtgatgg agcctttttt aaagataaag
    11581 aagttgctat agtaggtgga ggaaatactg cactagaaga tgctttattt ttagctaata
    11641 attgtacaaa agtgtattta attcacagaa gagatagttt tagaggtgaa gaagtgttgg
    11701 agaaatctgt gaaagcaaga gagaatattg agatacttta cagtcatggt gtagaaaaaa
    11761 tagaaggaga aaagactgta tcgaaaatag aagtaaaaaa cttaaagact gaagaaaaaa
    11821 gaactataga tgttagtggt atttttattg ctataggtct taaacctaat aataaaatgt
    11881 ttgaaaatgt tcttgatttg gatgaaggtg gatatataat ttcagatgag tcttgtacaa
    11941 catcagtaga aggtgtatat gtagcaggag atagtagaac taagttttta cgtcaaataa
    12001 taacagcagc atcagatgga gctattgcag ccgttcaagc agctaattat ataaatgttg
    12061 aataatataa atttcaaata aaaatagcca tataaactga aataattttt cagtactata
    12121 tggctatttt gttaattaga ggtaaatgta cgttttattt accttgtcta gatttgttat
    12181 atcttaatct atcatgttca tttaagaatc tttttctaag tcttatagaa tcaggtgtaa
    12241 tttcaactaa ttcatcatct tctataaact ctagtgcttc ttcaagagta aatattctag
    12301 gaggagataa ttttatagca tcatctgaac cagaagcacg tacgttactc attttcttgt
    12361 ttttacaagg gttgactacc atatcatctt ttctggaatt cataccaatt atcatacctt
    12421 cgtaaacttc tacaccaggg tctacaaaca ttacagctct atcacttaag gcattaagag
    12481 aataccccat agtaacacca ggtccttgtg ctattaaaac accatttcct cttgaaggta
    12541 tttctccttt aaattcttca aatttttcaa atgaacgaac taaagtacct tctccacgag
    12601 tagcgtttat aaattcactt cgatatccta aaagaccacg agtaggtgct aagaactcta
    12661 tttttacata gtctccttct attgacatag actccatcat accttttctc atgtttaatt
    12721 catttataat agtaccagag taaacttcag gacagtttac aacaactctt tctattggtt
    12781 ccattagttt tccatcttct ttatgcatta aaacttcagg tttagaaaca ccaacttcat
    12841 aaccttcacg tctcatattt tcaagtagta tagataagtg aagctctcca cgtccagaaa
    12901 ctttgtatcc atcagtagta tcaagaggtt caactttaag accaacattt acttcaagtt
    12961 ctttttcaag tctatctttc aaatgtctag ttgtaacgaa ttttccactt tttccaacga
    13021 atggagagtc gtttactaag aagttcatag aaagagtagg ctcttctatt tttatcattt
    13081 ccataggtag tggactatct aaatcgcata tagtttcacc tatagatata tcaggaatac
    13141 cagctataac aactatttct ccactagtag cttcatcaac ttcaacttgt tttaaacctt
    13201 catacacaga aagttttgat acttttcctt tagatacaac agaatcttct ctacatatag
    13261 caacttgtgt attgttttta agagtacctt tgtaaattct acctatacca agtctaccta
    13321 cataatcatc atatgcaaga gctgatattt ggaactgtag aggttcatta tcgtaattag
    13381 gataagcttc aacgtgattt acaatagttt taaatagagg agaaaggtct tcactatcat
    13441 catccatttc aagtttagca ataccttgtt tagcaatacc atatattata ggaaattcgc
    13501 attgctcatc tgttgcattt aagtctacaa ataaatcgaa aacttcatta actacttctt
    13561 ccgctctctg gtctttttta tcaatcttgt ttataaataa tattggtttt aaaccgaact
    13621 ctaaagattt ttgtaaaacg aatctagttt gaggcattgg gccttcacta gcatcaacta
    13681 atagaataac tgtgtcaaca gttttcataa cacgttcaac ttcagaagaa aagtcactgt
    13741 gtcctggagt atcaactatg tttattttgt aatcttcata gtttattgca cagttttttg
    13801 aatatatagt tatgcctctt tctttttcaa ggtcgttact gtccataacg cagtctttaa
    13861 ctacttcgtt ctttctaaaa acaccgcttt gagaaaggaa agcatcaacc aatgttgatt
    13921 ttccagcgtc aacgtgggct ataactgcga tatttataat tttatgtttt tgtgacattt
    13981 ttgttgctcc cttcgattaa aataacttat taattatagc acaatatatt attaggtaca
    14041 aggaggaata tattaaattg tattaatata gtttaaattg gataaaactg ttagttttgc
    14101 atctgaaaat tattctgttt aaaaaaattt tttagacaaa taataacctt aatagataaa
    14161 aagattaata aaagagtaaa ggatgatata tgttggaagt aaccaaaata ataattaaat
    14221 attttgcaag tgaaagtgat acttttgtaa aagaagaagt ttgtaatttt gaagaacttc
    14281 ttaagtggtc tcataaattt attgaaggta aaaagataga agatgttttg tatataaagt
    14341 ctaaagaaaa ctataaagta aatgtgatta agtctttaga aattaaggaa ttggaaatag
    14401 tgagaaaaca acagatacca ccgtacatgt agtattaata atatataaaa ttaatatgta
    14461 atactcgcaa taggatataa atggtatata tggtaataat ttatattaaa aaaactttta
    14521 taaagtttat atttgggtat aaaagtatta attagacatt aattaggagg aacttaaata
    14581 tgataagagt atgtccaaac tgttcaaatg tggatgtaaa taagctaaaa actttggttg
    14641 gagaagaaaa tgtaaagact ggttgtatag gtcagtgtag agcatttaaa aaagaagctg
    14701 ttggaataat agacaatgag cttgaaataa aggaaacaga agcagagttt tttgaagcat
    14761 gtaaagaata aattttaaat aaaatataat aaatagagca tctaaattgt ataagcatta
    14821 aacaatttag atgctctatt tttatatata catttaatta tgagtatact tttctattaa
    14881 atacaaataa atataactaa tacatttatt tat
//
LOCUS       DBGJFF010000092        14707 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.093, whole genome shotgun sequence.
ACCESSION   DBGJFF010000092 DBGJFF010000000
VERSION     DBGJFF010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14707
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.093"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(23..601)
                     /locus_tag="ACOIAF_002731"
     CDS             complement(23..601)
                     /locus_tag="ACOIAF_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGZ2669735.1"
                     /translation="MAYAKYLTKITDINKFYLENIINEGDIVIDATMGNGYDTRYLAE
                     KVGEKGFVYAFDIQEEAIKSTRKKLEKEGYIDRVKLIFDGHEKMNQYIKEEVSCVLFN
                     LGYLPRAKHSIITKPDTTLKAIKLSLEMLKENGVISIAIYTGHDGGQEEKDCIYNLVN
                     NLNQDEFNVLESKFLNQVNNPPQLILIEKKKA"
     gene            complement(659..1375)
                     /locus_tag="ACOIAF_002732"
     CDS             complement(659..1375)
                     /locus_tag="ACOIAF_002732"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HGZ2669736.1"
                     /translation="MRINKYIASCGIASRRKAEEIILGGRIKVNGSVVKELSFNIDEE
                     KDIVEFDNKKVKPSENYIYIVLNKPEGYITTVKDQFNRPSVIDILKDVRERVYPIGRL
                     DYETSGLLILTNDGDLTYKLTHPKHEIDKTYVASVKGIISDDEIRKFETGLKIEDYTT
                     APAKIRVIKENKEKNYSVCEITIHEGRNRQVRKMCKAINHPVLNLRRISVGKIVLKDT
                     KVGEYRYLTEDEIKYLKSIK"
     gene            1611..2651
                     /locus_tag="ACOIAF_002733"
                     /pseudo
     CDS             1611..2651
                     /locus_tag="ACOIAF_002733"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
     gene            complement(2807..3466)
                     /locus_tag="ACOIAF_002734"
     CDS             complement(2807..3466)
                     /locus_tag="ACOIAF_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669737.1"
                     /translation="MRGRVILLISILSIFLVGCSFNKKDEINLVEQGKTYLEKHNYKK
                     AMESLSSALEEDSTNENARAMYMQAMRMSNMTEFEELKNYEGAIKELKLIEKIKNGSS
                     VIKEEATKKKEELTKLEKEQNEAEEQRKKKAKDTAGEDRYRVESDARKSSYSGSSKNN
                     KSSSKNNDKKSDSNKSDNNNSSSQNQEKPNNSNPTPAPTPQDTSGGSDSGGNSPNSNN
                     Q"
     gene            complement(3683..4975)
                     /gene="lysA"
                     /locus_tag="ACOIAF_002735"
     CDS             complement(3683..4975)
                     /gene="lysA"
                     /locus_tag="ACOIAF_002735"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HGZ2669738.1"
                     /translation="MKLHGTMTVNNDELYIGGVSCLELSKKYQTPLYVFDEALVRQNC
                     REYIEYFKAREGKNKVAYAGKAFLPLYMCNLINEEQMCLDVVSGGELYTAYKSNFPME
                     KVFFHGNNKTKEEINMGLDLGVGKFVVDNFYELDLLEALCEDKGKTQEIYFRITPGIE
                     AHTHDYIKTGQIDSKFGFALVNGDLFRAIEKLSEYKNIKLVGLHAHIGSQIFDVEPYI
                     DTVDIMMNLVKEIKEKFNIQLTEIDLGGGVGVYYSEGDKPKTIKEFCETIITKAETSC
                     KELGIEVPTLVIEPGRSIVANAGSTLYTVGSIKDIKDVRVYVSVDGGMTDNVRPSLYQ
                     ASYECSIVNKINHEAMNHVTIAGKCCESGDILIGDINIMDIKSGDILITTSTGAYGYS
                     MSSNYNKIPRAAVVSVFDGNDKIICKRQSYEDLLALEV"
     gene            complement(4993..6186)
                     /locus_tag="ACOIAF_002736"
     CDS             complement(4993..6186)
                     /locus_tag="ACOIAF_002736"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HGZ2669739.1"
                     /translation="MSIIVQKYGGSSVADTEKIKSIAENIIERRKENPQMVIVVSAMG
                     KSTDEYITLAKELSNEPSKRELDALMSTGEMISASLLSIALNALGCKAISYNAYQLNI
                     KTSGLHGKSQIDDINVNRIKNSLDEGNVVIVTGFQGINEDGDVTTLGRGGSDTSAVAL
                     AVKLNGKCEIYTDVDGIYFTDPRKYSKASKLDEIEYEEMLELASLGAQVMHSRSIELA
                     QKYGVEIYVGRTCGTEKGTYIRGGKDMKLEDKVITGLATSDDDSSITIKDFKAENISS
                     LFEDIATIGISVDMISQTAPILDKISVSFTVPKEELGECKKIVSKYTDEEHVVIDNNI
                     TKFSLVGLGMKNTSGVAAKVFKIFNENGIMIKLITTSEIRITCAINSDDKQVAIEKIA
                     EVFNI"
     regulatory      complement(6268..6436)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            complement(6547..7164)
                     /locus_tag="ACOIAF_002737"
     CDS             complement(6547..7164)
                     /locus_tag="ACOIAF_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669740.1"
                     /translation="MTNMEKEILLLISRVRCVTESQFRKIYGDQKRYNKKNFKKTLRK
                     MCSEYTLKKYPCDVDYYNYKDLSYVYYLNGSKLFKGDDLIKALVGSELVVRINTAGCD
                     VKRFYRNVSVDDVKYDLFIEYTDQFGNIKQVLVDVDLNNEIDISKYENLSKKIDKSTI
                     PFFKVPNVLVVTPDNLSELNDLERYDNEYSINFIDLGLNKLFNCI"
     gene            complement(7334..9034)
                     /locus_tag="ACOIAF_002738"
     CDS             complement(7334..9034)
                     /locus_tag="ACOIAF_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669741.1"
                     /translation="MKRYLKTYGVALIIVIIYAFIKLPVLRLDFTSLFSVGIIFFVVA
                     GILDMMLDRNERASKMAKYNFSIAIALLVYIVVVPFITSTPVLHAKSYRELLGKVTES
                     KFTDDISPVSVNDIRLVDEDMAMKLGDKKLGEVPAIGSVSKLGKFHIQNVDGELYWVA
                     PLVHRDIIKWITSLSGTSGYVKVSASNPQDVQLVQEIDGKPIKIVYQPEAYLHQDLQR
                     HLYIHGIVNVGMTDFTLEINDEGRPYWVVSLYEHKIGYGGANATGIATVDAETGKINV
                     YDVKNTPKWVDRIQPQNFITDQIKDWGVYVNGFLNSVISEKGVLVPTEGTSLVYGNDN
                     RSYWYTGITSSGGDESTIGFMLVDSRTKEAKLYKQPGATETAAMKSAEGNVQEKNYEA
                     TFPVMYNILGQPTYVSSLKDKAGLVKRVAFVSVEDYNVLGIGEDKNEALRNYKDALES
                     KGNDLKLDNDLKDEVIEGTVTRISPDVRGGNTNYYVTLDSNKDIIFRATSKVSSELPL
                     TQVGDKVKISYSKEESGVIEMSEFDNLNIANFEDKKEKEKTGTDENTGSQDSENQGNV
                     "
     gene            complement(9431..9994)
                     /locus_tag="ACOIAF_002739"
     CDS             complement(9431..9994)
                     /locus_tag="ACOIAF_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669742.1"
                     /translation="MNKVYIVTTYTGTILSYLIRNISKKLYTHVSISLNENLKPMYSF
                     GRLNPRNPFIGGFVEENINQGLYAIRKNTVCRVYSLEVDNLQYENLYKNIKLISDYRE
                     DYYYDTMALIYLPMNIQRQSEYKYVCSNFVADMLEKSEIEILNKQAFEVTPNDFYNLS
                     GLTLEYEGLLSEYNSRQHSYVSKIANI"
     gene            complement(10155..10868)
                     /locus_tag="ACOIAF_002740"
     CDS             complement(10155..10868)
                     /locus_tag="ACOIAF_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2140 domain-containing protein"
                     /protein_id="HGZ2669743.1"
                     /translation="MGKVAKILLSLIVIVLVLAGIVVYILTPKERYDVSSQNNKIIDE
                     EYLSKGSYINSMEVVKNPLRMVGKISVSEEEFKNLIYTLMQKHGINELENNFVEMKSG
                     KIKVAGPYKVFGLVNSQYELELRPTLKDGNLVVSLENVKLGKFKLSDKMLEKILSNYN
                     KKVPFEVNGNKITLEKSYLYPITLKNISIKKGNVDLDMEVEIDLKSQINGALGYLKDS
                     GKVQDILNHLINLKKTSVG"
     gene            11160..12824
                     /locus_tag="ACOIAF_002741"
     CDS             11160..12824
                     /locus_tag="ACOIAF_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HGZ2669744.1"
                     /translation="MSNETNSSNFIKNIIINDLETGKHDSIITRFPPEPNGYLHIGHA
                     KSICLNFGLAKEFNGKANLRFDDTNPLKEDVEYVESIKEDVKWLGFDWNELNFASNYF
                     DEMYKRALILIKKGKAYVCDLTQEEMREYRGTLTEPGKESPHRNRTIEENLDLFERMK
                     NGEFKDGEKTLRAKIDMSSPNINLRDPIIYRISHSTHHNTGDKWCIYPMYAFAHPIED
                     AIEGITHSICTLEFEDQRPLYDWFVKECEMENIPRQIEFARLNINNTVMSKRKLKQLV
                     DEGIVDGWDDPRVPTISGIRRKGYTAEALRNFCSEIGVSKVNSTVDSQMLDYFLRENL
                     QPKAPLTMGVLRPLKLIITNYPEDKIEMLEIENNAKDESQGKRLVPFSRELYIEQDDF
                     MEEPVKKYFRFFPGNEVRLKGAYFVKCTDVIKDENGNVVEIHGTYDPETKSGSGFTGR
                     KVKSTIHWVDAKSAIPCEFRLFEPLILDDIPENEGKHFLEQINPNSLEILQGFVEPTQ
                     IKDAKPFDKFQFVRNGFFSIDNKYTTDEKFVFNRIVPLKSSFKPGK"
     gene            complement(12931..13830)
                     /locus_tag="ACOIAF_002742"
     CDS             complement(12931..13830)
                     /locus_tag="ACOIAF_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HGZ2669745.1"
                     /translation="MKKVKLIYNPNSGEKKILSNLDTIIELYQEKNYLLIPYRLTIKE
                     PVKNAFKEVDSSYEHILIAGGDGTVDLVVNAMKELDIKIPIGILPTGTANDFSNALQI
                     SFDIKEAINEIINSKPKKIDIGKVNNKYFINVASAGMFTDVSQRINTDLKNSFGRISY
                     YIKGIEEALYMRKFKIKVSSEEMYYDGDMYLMLIFNGKTAGNINLAYKAEIDDGYLDV
                     IIFKGMPIPKSIPVLISVLRGDYLDQYNGNEILYFKTKKVHIECEDSLITDIDGEKGP
                     DFPLNIECIKDGIEVMGICSEKS"
     gene            complement(13857..14495)
                     /locus_tag="ACOIAF_002743"
     CDS             complement(13857..14495)
                     /locus_tag="ACOIAF_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HGZ2669746.1"
                     /translation="MDQNLNWLNEKRIEKTIKSLENNNMKGYLVSNKEELLKKIEEIV
                     KAGSLVGCGGSMTLFETGVIDFIREKYNFLDRYAKDIKPEEMKEIYRKSFSADAYFTS
                     TNAITEDGELYNLDGNGNRVAAMIYGPDKVIVIAGVNKIVKDVDEAIKRTKTIAAPAN
                     CKRLSTNTGCKTTGVCIDCSSPGRICCSYTLIKRQLTPERIHVIFLNEDFGY"
BASE COUNT         4729 a         2464 c         1597 g         5917 t
ORIGIN      
        1 ttgtcaagtt ttttagtatt tattatgctt ttttcttttc aattaaaatc aattgtggtg
       61 ggttattgac ttgatttaaa aacttagatt ctaaaacatt aaattcatct tgatttaaat
      121 tattgacaag gttatatatg caatcttttt cttcttgacc tccatcatgc cctgtatata
      181 tggcaatgct tattactcca ttttctttta acatttctaa gcttaacttt atagccttta
      241 aagttgtatc tggtttggtt ataattgaat gtttcgctct tggaagataa cccaaattaa
      301 ataaaacaca tgaaacttct tcttttatat attgattcat tttttcatgc ccatcaaata
      361 tcaattttac tctatctata tatccttctt tctctagttt ctttcttgta gactttattg
      421 cttcttcttg tatatcaaaa gcatatacaa agcccttttc tccaaccttc tcagctagat
      481 accttgtatc atacccattt cccatagtag catcaattac tatgtcacct tcatttataa
      541 tattttccaa ataaaattta tttatatctg taattttagt taaatacttt gcatatgcca
      601 taatactatt tctccctact attaacttta tttcattttt tctccattta tttttatctt
      661 attttattga ttttaaatat tttatttcat cttctgttag gtatctatat tctccaactt
      721 tggtgtcttt taatactatt ttccctacag aaatccttct taaatttaaa actggatgat
      781 ttatagcttt acacattttt cttacttgtc tatttctacc ctcatgtata gttatttcac
      841 acactgaata atttttttct ttattttctt taattaccct tatcttggca ggtgctgttg
      901 tgtaatcttc tatttttagt cctgtttcga attttcttat ctcatcatca cttataattc
      961 ccttcacact tgccacatac gttttatcaa tttcatgctt tggatgggta agcttatatg
     1021 ttaaatcacc atcattagta agtattaata atccactagt ttcataatct aatctcccta
     1081 ttggatatac tctttccctt acatctttaa gtatatctat tacactgggt ctattgaatt
     1141 ggtcttttac tgtagttata tatccctctg gtttatttaa tactatatat atataatttt
     1201 cacttggttt tactttttta ttatcaaatt ctactatatc tttttcttca tcaatattaa
     1261 aagatagctc ttttactaca cttccattta cctttattct tcctccaaga attatttctt
     1321 ctgcttttct tctggacgct attccacaag aagctatata cttattaatt ctcatactta
     1381 atccttcctt atcttaatta tgtgttgttt aatatttcta tcaatgttaa ttttgctaca
     1441 ttattatact atactagacc ttaattaaaa acaagccaaa aaattattta attttcctaa
     1501 ataattgtaa tttcttttca catactatta atatgtaata gctgactaaa cactatttta
     1561 aggaagtgat tattatttca aaggaaaata taaaatatta tttaatagtc gtgtttattg
     1621 gaattttatt ttttaaattt attaatactc catcagactt tatatctagt attgaaggat
     1681 tcttaaagtt ttttagccca tttttattag ctattttact tgctttactt ttaaatccac
     1741 ttgtgatgct ttttgaggta aagttcaaag cacatagact tttagccatt tttttatctt
     1801 atatatttat aggttttatt ttagcctttg ccattaggtt attaattcca tcaatagcta
     1861 atacattaaa tagattgata aatgaaatgc ctatgtaaac tgattatata gatagtttta
     1921 tagaaaaaaa tatgtcaaac atagattttc taaaaacttt aattcctcat atacaacata
     1981 gtttagataa tttattaaaa gaagcaagta attttattgg taaaattcct aaaaacttct
     2041 taacctacac acttagtata tcttctatgc tatttaatat gacaatggga tttatactat
     2101 ctatatacat tatatatgac aaagaaaaaa ttgcattggg atttaagaga tttctatatt
     2161 cttctactgc tagaaacaag gcagataaca ttatagaatt ttttaggact actcatgata
     2221 ttttctatga ttatttactt ggtagaatat tagactctct tattattggt ataattgcat
     2281 ttttaggatt tcaatttgtt atcagaatag aaaatgcttt atttttagct tcaataatat
     2341 tcttaggtaa tataattccc tattttggtc cttttattgg tgcaatccct ccaatagcca
     2401 tgactatatt atatagtcca caaaaaacta tatgggtcat tgcatttcta tttatattgc
     2461 aacaattaga tggaaatttt atagaaccaa aagttatggg taatcaagtt ggtataggag
     2521 ctatatgggt aatatctgct attttggtag gtcaatctct atttggtttt ataggagttt
     2581 ttctttcagt accaatagct gctgttgtaa aaacttatgt tgataaatat atagataatc
     2641 gtttacaata gtttatctct actgtgactt taatttttag tttaaaattt aactgttatt
     2701 tattaaatta tttctcaaaa aaatatctct tagtaagaaa agattgctaa gagatatttt
     2761 ttataatata tattttgttc attaaattta tgtatatatt ttatacttat tgattatttg
     2821 agtttggaga atttccacca ctatctgaac caccactagt atcttgtgga gttggtgctg
     2881 gtgttggatt actgttattt ggcttttctt gattttgaga agaactgtta ttattgtcag
     2941 acttattact atctgacttt ttatcattat ttttagaact agatttatta tttttactac
     3001 ttccactata agaagatttc cttgcatctg attcaactct atacctatct tctccagccg
     3061 tatcttttgc ctttttcttt ctttgttcct ctgcttcgtt ttgttctttt tctaatttag
     3121 ttaactcttc tttcttttta gttgcctctt ctttaataac agaggaacca tttttgattt
     3181 tctctataag tttaagttcc ttgattgcac cctcataatt ttttaattct tcaaactccg
     3241 tcatatttga cattctcata gcttgcatat acatagctct tgcattttca ttagtactat
     3301 cttcttccag tgcagaggat aatgattcca tagctttttt atagttatgt ttttctaaat
     3361 atgtttttcc ttgctctact aaatttattt catctttttt attaaatgaa catcctacaa
     3421 gaaatataga taatatcgat attaataaaa ttactcttcc tctcattaaa tccctccttg
     3481 tcgtatactt taaattttat cataattata aagtttttaa aatattttat atttattaca
     3541 agaaatttgt tagtaaaaca aaaaataagc agatatgcta cgaacgatac aataaaccaa
     3601 aaattaattt agagatacat atcctagcaa atctgctcat aataaacttt aatatttaat
     3661 ttttattata ttcttctcca ttttatacct ctaaagctaa taagtcctca tagctttgtc
     3721 tcttacaaat tatcttatca tttccatcaa atactgatac tactgctgct cttggtattt
     3781 tattataatt tgaagacatt gaatatccat atgcaccagt tgatgttgtt ataagtatat
     3841 ctccactttt aatatccatt atgtttatat ctccaattaa tatatctcca ctctcacaac
     3901 attttccagc tattgttaca tgattcatag cctcatgatt aattttattc acaatactac
     3961 actcataact agcttggtac aacgaaggtc ttacattatc tgtcatacct ccatctacac
     4021 ttacataaac cctaacatct tttatatctt ttatagagcc tactgtatac aatgtacttc
     4081 ctgcatttgc aactattgac cttcctggtt ctattaccaa cgtaggtact tctattccaa
     4141 gctctttaca tgaagtttct gctttagtga ttatagtttc acaaaactct tttatagttt
     4201 taggtttatc tccttcgcta taataaactc caactcctcc acctaggtca atttctgtta
     4261 gttgtatgtt gaatttttct tttatttctt ttaccaagtt catcattata tccactgtat
     4321 ctatgtatgg ttctacatca aatatttgtg aacctatatg agcgtgaaga cccacaagtt
     4381 taatattttt atattcactt aatttttcta ttgctctaaa taaatctcca tttactaaag
     4441 caaatccaaa tttagaatct atctgtccag tttttatata atcatgtgta tgagcttcta
     4501 taccaggtgt tattctaaag tatatctctt gagttttacc tttatcttca cataatgctt
     4561 caagcaaatc taattcataa aaattatcca ctacaaattt acctacacct aaatctaatc
     4621 ccatgtttat ttcttcttta gtcttattgt ttccatgaaa gaaaactttt tccataggaa
     4681 aatttgattt ataagctgta tataactcgc ctcctgaaac tacatctaaa cacatttgtt
     4741 cttcatttat aagattacac atgtaaagag gtaaaaaagc tttccctgca taagcaacct
     4801 tattttttcc ctctctagcc ttaaaatatt ctatatattc tctacaattt tgtctaacta
     4861 aagcttcatc aaatacataa agtggagttt gatatttttt actaagttct aaacaactca
     4921 ctcctccaat atataattca tcattattta cagtcatagt tccatgaagt ttcataatag
     4981 cctccccaaa ttttatatat taaatacttc tgctattttt tctatagcta cttgcttatc
     5041 atcagaattt atagcacaag ttatacgaat ttctgatgta gttataagtt ttatcattat
     5101 accattttca ttgaatatct taaatacttt agcagcaact ccagaagtat ttttcatacc
     5161 aagacctact aaagaaaatt tagtgatatt attatcaatc actacatgtt cttcatctgt
     5221 atatttagat actatctttt tacattctcc caactcttct tttggaactg taaatgatac
     5281 acttatctta tctaaaatag gtgcagtttg gcttatcata tctacactta tacctatagt
     5341 tgctatatct tcaaataagc tagatatatt ttcagcttta aaatctttta ttgttataga
     5401 actgtcatca tcactagtag ctaatcctgt tattacttta tcctctaatt tcatgtcttt
     5461 tccccctctt atatatgttc ctttttctgt accacatgta cgtccaacat agatttcaac
     5521 accatatttt tgtgcaagct ctatacttct tgaatgcatt acttgtgctc ctaaactcgc
     5581 taactcaagc atttcttcat attcaatctc atcaagttta ctagcctttg aatattttct
     5641 tgggtctgta aaataaattc catcaacatc tgtatatatc tcacattttc catttaattt
     5701 aaccgcaaga gctacagcag atgtatctga accacctctt ccaagagtgg ttacatcacc
     5761 atcttcgttg attccctgaa atccagttac tatcactaca tttccttcat ctaaactatt
     5821 tttaattctg tttacgttta tatcatcaat ttgagacttt ccatgaagac cactcgtttt
     5881 tatatttaac tggtaagcat tataacttat agctttacat ccaagtgcat ttaatgctat
     5941 tgataataaa gatgctgaaa tcatttcacc agtagacatt aaagcatcta attccctctt
     6001 actaggttca ttactcaatt cctttgccaa agttatatat tcatcagtgg attttcccat
     6061 ggcagacaca actattacca tttgtggatt ttcttttctt cgttctatta tattttctgc
     6121 tattgatttt attttttcag tatctgctac tgaacttcca ccatattttt ggacaattat
     6181 actcatgctt tctccccctc aagctaaatt ttgataaata aaaacgccca aaggtatact
     6241 ttggacgtgt aaatttcata gaccaagtaa ccttgtagct caccacttta aaagtgacag
     6301 ccttatgcat attctgcata agtccagaaa atttgtccgt caacaaaatt tcttcggcta
     6361 tatacccttt catcaattat caacgcttac cagctcctat tgaatactat tgaatacagc
     6421 tacctctacc ctaggctcat tatttttaat taatttatta atatagtttt atcatattct
     6481 atataatatt gcaacaatta ttttacaaaa tgtggcaaat cgatagtttt cttttctaaa
     6541 gttagcttat atacaattaa atagtttatt taatcctaaa tctataaaat ttatgctata
     6601 ctcattatca tatctctcta aatcatttaa ttcggataga ttatctggtg taactaccaa
     6661 aacgtttggt actttaaaaa atggaattgt agatttatct atttttttag aaagattttc
     6721 gtatttagaa atgtctattt cattatttaa gtcaacatca actaaaactt gctttatatt
     6781 accaaattgg tctgtatatt caataaataa atcgtatttc acatcatcta cactaacatt
     6841 tcggtaaaat ctttttacat cacaacctgc agtattgatt ctaacaacta attctgatcc
     6901 tactagagcc tttattaaat catctccctt aaaaagctta ctaccattta aatagtatac
     6961 ataagagagg tctttataat tataataatc cacatcacaa ggatattttt ttagtgtata
     7021 ttcactacac attttcctca aagttttttt aaaatttttt ttattgtatc ttttttggtc
     7081 tccataaatt tttctaaatt gagactctgt aacacatcta accctgctaa ttaaaagtaa
     7141 tatctctttt tccatattcg tcatagtaca ttctccttga agtattattt gcaaagttgt
     7201 actaatatta tattaatttt atgacattaa atttacaaag aattaaaata aaatagctaa
     7261 tttttataga atttaattct ataaaaatta gctattacta tgctaaatac tgttatcaat
     7321 ctttttattt aaattataca ttaccttgat tttcagaatc ttgggaacct gtattttcat
     7381 cagttcctgt tttttctttt tcttttttat cttcaaagtt tgcaatattt aggttatcaa
     7441 attctgacat ttctattaca ccagattctt cttttgaata cgatattttg actttatcac
     7501 caacctgagt taatggcaat tcgctagata cttttgaagt agccctaaat attatatctt
     7561 tatttgaatc taaagttaca taataattag tgttaccacc tcgtacatct gggcttattc
     7621 ttgtcactgt gccttctatt acctcatctt ttaaatcatt atcaagtttt aaatcattac
     7681 ccttagattc taatgcatct ttatagtttc ttaatgcttc attcttatct tcacctattc
     7741 caagtacatt ataatcttca acagatacaa atgcaactct cttaacaaga ccagccttat
     7801 cttttaatga tgatacatat gttggctgac ccaatatatt atacattaca gggaatgtag
     7861 cttcataatt tttttcttga acattacctt cagccgattt cattgcagcc gtttcagtag
     7921 ctcctggttg tttatataat ttagcctctt ttgttctaga atcaactaac ataaatccaa
     7981 ttgtagattc atctccacct gaagatgtta tacctgtata ccaataagac ctattatcat
     8041 ttccataaac taatgatgta ccttctgttg gtactaaaac tcccttttct gatataacag
     8101 aatttaaaaa tccattaaca taaacacccc agtctttaat ctggtctgtt ataaagtttt
     8161 gtggttgtat tctatctacc catttaggtg tgttcttaac atcataaaca tttatttttc
     8221 cagtttctgc atcaacagtg gctattcctg tggcatttgc tcctccatag ccaattttat
     8281 gttcatataa acttacaacc caataaggtc ttccttcatc atttatttca agagtgaaat
     8341 ctgtcatacc aacatttaca ataccatgta tatataaatg tctttgtaaa tcttgatgta
     8401 aataggcttc tggttggtaa actatcttta ttggctttcc atctatttct tgaactaatt
     8461 ggacgtcttg aggattacta gctgatactt ttacataacc acttgtacca cttagagatg
     8521 ttatccattt aattatgtct ctgtgaacaa gaggcgctac ccagtacaac tctccatcta
     8581 cattttgtat atgaaatttt cctaatttgg atacactccc tattgcagga acttctccaa
     8641 gctttttgtc acctaatttc attgccatat cttcgtctac aagacgaatg tcatttacac
     8701 tgactgggct aatgtcatca gtaaatttac tttcagttac ttttcccaat agttctctat
     8761 atgattttgc atgaagtaca ggtgtagaag ttataaaggg aactactaca atatacacaa
     8821 gcaaagctat tgctattgaa aaattatatt tagccatttt agatgctctt tcatttctat
     8881 ctaacatcat atctagtatt cctgctacaa caaagaaaat tattcctact gaaaataaag
     8941 atgtaaaatc taatcttagt acaggtaatt taataaatgc atagattatt acaattatta
     9001 atgcaactcc atatgtttta agatatcgtt tcatttaata ctcctctcta aaaaatgtta
     9061 ttattactat tatatccata tatttacaaa aaataaatat ttatcatatt tattatatat
     9121 taagatatct ttatatattt atagttctgc ttattcaact atatttaaca ttatatgtgt
     9181 atgtataaat actttttata cactgtttgt gctatccatc ccacacctaa aggagtagga
     9241 tttccgtact attctgtatt atatatatcc aaaaatatta taaaaataca aatattagca
     9301 gaaatttaaa aactgaagaa agtattctaa ctaagattta caaattctta atcctatcga
     9361 agttaaacta ctatcttcag cctaattatc tagcaatatt tttttatatg aaaaactcta
     9421 aatcaagttt ttaaatgttt gcgatttttg atacataaga atgttgtcta gaattatatt
     9481 ctgataataa accttcatac tctaaggtta agccagataa attataaaaa tcatttggtg
     9541 ttacttcaaa tgcttgttta tttaaaattt ctatctcact tttttctaac atgtcagcaa
     9601 caaaatttga acatacatat ttatattcag attgtctctg aatattcatt ggtaaatata
     9661 ttaatgccat tgtatcgtaa tagtaatctt ctctataatc actaattaat tttatatttt
     9721 tatatagatt ttcatattgt aaattatcta cttctaacga atatactcta cacacagtat
     9781 tttttcttat agcatataat ccttgattta tattttcttc aacaaatcct ccaataaatg
     9841 gattccttgg atttagtctt ccaaaagaat acattggttt tagattttca tttaaggata
     9901 ttgacacatg tgtatacagt tttttactta tattccttat taagtaagac aatatagttc
     9961 ctgtatatgt tgttacaata tagactttat tcatcatttc cccccaatct agtaactaaa
    10021 taatattaca cacttacaat ttaatatcat aagatattct aacataataa atgtggtttt
    10081 acaaattttg attttaaggt actttaatat tatataaaat ataataaaaa tataaatata
    10141 agttttctat atttttaacc aacagatgtt ttcttcaaat taatcagatg atttagtata
    10201 tcttgaacct ttccagagtc ctttaaatat cctaatgccc catttatttg tgattttaaa
    10261 tctatttcta cttccatatc caaatcaaca tttccttttt ttatagatat atttttaagt
    10321 gttattggat aaagataact tttttccaaa gtaatcttat ttccattaac ctcaaaggga
    10381 acttttttat tgtagttact taatattttt tctaacattt tatcacttag cttaaatttt
    10441 ccaagtttca cattctcaag acttacaact aagtttccgt cctttaaagt aggtctcagt
    10501 tcaagttcat attgactatt taccaaacca aataccttat atggtcctgc tacctttatt
    10561 ttaccacttt tcatttcaac aaaattattt tcaagctcat taattccgtg cttctgcatc
    10621 aatgtatata ttaaattttt aaactcttcc tctgaaacag atatttttcc aaccattcta
    10681 agtggatttt ttactacctc catagaattt atatatgagc cttttgataa atattcttca
    10741 tctatgattt tattattttg tgaactaaca tcatatcttt cttttggtgt caagatatat
    10801 acaacaattc ctgcaagtac taaaacaata acaattaaac ttaacaaaat ctttgcaacc
    10861 tttcccataa tcgtctcctt tacaaatctg tcatcagatt taaaataaat ttagataact
    10921 aacttcacat aatattctta acaatataca tactataata cagtctttta aaaattaatt
    10981 caaccaatct aatgttttgt aaataaactg taaaaacatg tttttagtat aaaagttaat
    11041 attttgcata atactcttaa ttacaccttt atttttttct aatcttggat taaaattaat
    11101 attatgcaat acagtgtagt gttctgtatt ataatcaaat taaataggag gtatagaata
    11161 tgtcaaatga aacgaactca tctaacttta taaaaaatat tattataaac gacttagaaa
    11221 caggaaaaca tgacagtatc ataactcgtt ttccacctga gccaaacgga tatttacata
    11281 ttggtcatgc aaaaagtata tgtcttaatt ttggattagc taaggagttt aatggaaaag
    11341 ctaatttaag atttgatgat acaaacccat taaaagagga tgttgaatat gtagaatcca
    11401 taaaagaaga tgtaaagtgg ttaggatttg attggaacga gttaaatttt gcatctaact
    11461 actttgatga aatgtataag agagctttaa tactaataaa aaagggtaaa gcttatgtat
    11521 gtgatttgac acaagaggaa atgagagaat atcgtggtac tctaactgaa ccaggaaaag
    11581 aaagtcctca tagaaacaga actattgaag aaaaccttga tttatttgaa agaatgaaaa
    11641 atggtgaatt taaagatggt gaaaaaacat taagagccaa aatagatatg tcttctccaa
    11701 atataaacct tagagaccca attatatata gaatatctca ttcaacacac cataatacag
    11761 gagataaatg gtgcatatat cctatgtatg cttttgctca tccaattgaa gatgcaattg
    11821 aaggaataac tcactctata tgtactttag agtttgaaga ccaaagacct ctttatgatt
    11881 ggtttgttaa agaatgtgag atggaaaata tacctagaca aatagaattt gctagactta
    11941 atataaataa tactgtcatg agtaaaagga aattaaaaca acttgtagat gagggtattg
    12001 ttgatggttg ggatgaccct cgtgttccta caatatccgg aattagacgt aagggatata
    12061 ctgcagaagc attgcgcaat ttctgtagtg aaataggggt atcaaaagtt aactctacgg
    12121 tagatagtca aatgcttgat tatttcctaa gagaaaactt acaaccaaag gctcctctta
    12181 ctatgggagt tttaagacct ttaaaactta tcataactaa ttacccagaa gataagattg
    12241 agatgcttga aatagagaat aatgctaaag atgaatctca aggaaagaga ttagttcctt
    12301 tctctagaga attgtatata gaacaagatg attttatgga agaacctgtt aagaaatatt
    12361 ttagattttt cccaggaaat gaagtccgtt taaaaggtgc ttattttgta aaatgtactg
    12421 atgtaattaa ggatgaaaat ggaaatgtag ttgaaataca tggtacttat gaccctgaaa
    12481 ctaagagtgg ctctggcttt acaggacgta aggtaaaatc tacaatacat tgggttgatg
    12541 ctaaatcagc tataccatgt gaatttagat tatttgaacc actaattctt gatgatatcc
    12601 ctgaaaatga aggtaaacat ttcttagaac aaataaatcc taattcattg gaaatattac
    12661 aaggttttgt tgaacctaca cagataaaag atgctaaacc ttttgataaa ttccaatttg
    12721 ttagaaatgg attctttagt atagataata aatatacaac tgatgaaaaa tttgtattta
    12781 atagaatagt tcctctaaaa agttctttta aaccaggtaa ataatcttat aatcaaaaaa
    12841 tgaagactta aaatataaat tacttctact ataaataaac ttagttagaa gtaatttttt
    12901 atttgagtct tcatcattta ctttaaatga ttaacttttt tcactacaga ttcccataac
    12961 ttctattcca tcttttatgc attctatgtt aagaggaaaa tctggtcctt tttctccgtc
    13021 aatatctgtt ataagtgaat cttcacattc tatatgcacc tttttagttt taaagtatag
    13081 tatttcattt ccattatatt gatctaaata atcacctcta agcacactta tcaaaactgg
    13141 tattgatttt ggtattggca ttcctttaaa aataattaca tccaaatatc catcatctat
    13201 ttcagcctta tatgctagat ttatatttcc tgctgttttg ccattaaaaa tcaacataag
    13261 atacatatct ccatcataat acatttcctc agaagatact tttatcttaa atttcctcat
    13321 atacaatgct tcttctattc cctttatata ataagaaatt cttccaaatg aattttttaa
    13381 gtctgtgttt atcctttgtg atacatccgt aaacatacca gcacttgcaa catttataaa
    13441 gtacttatta ttgactttcc ctatgtctat tttctttggt tttgaattta tgatttcatt
    13501 tatagcttct tttatatcaa atgaaatttg aagagcattt gaaaaatcat ttgcagttcc
    13561 tgtaggcaaa attccaattg gaatttttat gtctaattct ttcattgcat ttactactaa
    13621 atctacagta ccatcaccac cagctatcaa tatgtgttca taagaactat caacttcctt
    13681 aaatgcattc tttacaggtt ctttaatagt taatctatag ggtatcaata aataattttt
    13741 ttcttgatat aattctatga tagtgtctaa atttgataat atcttttttt cacctgaatt
    13801 aggattgtat ataagtttta cttttttcat tatttctcct tactcatttt attcttttag
    13861 tatccaaaat cttcatttaa aaaaatcaca tgtattcttt ctggtgttaa ttgtcttttt
    13921 ataagtgtat aactacagca tattcttcct ggacttgaac agtctataca tacaccagta
    13981 gtcttacatc cagtatttgt acttaatctt ttacaattgg ctggagcagc tattgttttt
    14041 gttcttttta tagcttcatc tacatcttta actattttat ttactcctgc tataactatt
    14101 actttatcag gtccatatat catagcagca actctattcc cattcccatc taaattgtaa
    14161 agttctccat cctctgttat cgcattagta cttgtaaaat aagcatctgc agaaaatgac
    14221 tttctatata tttctttcat ttcttctggt ttgatatctt ttgcatatct atcaagaaaa
    14281 ttatattttt ctcttataaa atcaatcact cctgtttcaa ataaggtcat agaaccccca
    14341 catcctacta gagaacctgc tttaactatt tcttcaattt ttttaagaag ctcttctttg
    14401 ttacttacta aatacccttt catattattg ttttccaaag attttattgt tttttctatt
    14461 cttttttcat taagccaatt taaattttga tccattttaa cttccccctt atttttaata
    14521 tgtacttaat tatttataaa ctttattttc tttataagtt tattttagta catctaaaaa
    14581 aaaatacaat ataaaactta aatagtttta cataattttt tccaaaataa cttactcaac
    14641 caaaaaagga aagtagatgt actattctac tttccctata ttgtatgtag tattatatat
    14701 tgcctaa
//
LOCUS       DBGJFF010000093        14470 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.094, whole genome shotgun sequence.
ACCESSION   DBGJFF010000093 DBGJFF010000000
VERSION     DBGJFF010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14470
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.094"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(<1..812)
                     /locus_tag="ACOIAF_002744"
     CDS             complement(<1..812)
                     /locus_tag="ACOIAF_002744"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGZ2669747.1"
                     /translation="MLNKLCMYLERPELYKQSEINFWDDEYISKQLLKAHLDTNFEGA
                     SRNFNFIEDSVNWIVTVANPANYPKLLDLGCGPGLYAEKFAQKGYKVTGIDFSKRSIN
                     YAQNRNKETNLNINYLFQSYLNMNYNEEFDLATLIYCDYGALSTENRRLLMEKIYDSL
                     KPGGKLILDVFTINKYNNFEEIKYWEINEDGGFWSNEKYMCLQDNCKYNDYNTLEQTL
                     VITEKDENVFYVWNHYFSKESFLLEVENIGFKSVEFFSNVKGEAYSDDSMTMG"
     gene            complement(1164..2066)
                     /locus_tag="ACOIAF_002745"
     CDS             complement(1164..2066)
                     /locus_tag="ACOIAF_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HGZ2669748.1"
                     /translation="MYNKIKYIPINCEIACNKLKRSMPYKWDLNIYRGCEHGCKYCYA
                     IYSHKYINSNNYFEEIYVKTNIVEKLERQLKSSKWKKEVINIGGVTDSYQPIEADYKI
                     MPEILNLLIKYKTPAIISTKSDLILRDYDLIDKLSRITYINVASTITTVNEDTQRLIE
                     PNGVDSMRRFKMLKEFRKTNASVGLHIMPIIPYITDDFDNINSLFRHAKECNVHYVLP
                     GTLYLRGITRGVFFDFVKKEFPELLDKLSILYSAGSLNREYKNQLYKMVNELRYKYSL
                     SSSYSKIMKEKLKNSGDVQISFFD"
     gene            complement(2116..2577)
                     /locus_tag="ACOIAF_002746"
     CDS             complement(2116..2577)
                     /locus_tag="ACOIAF_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HGZ2669749.1"
                     /translation="MRLWHKDLIDVLPKNQLVSQWRELLAIKGSIDKKGTPNHLLVNK
                     VLNYSIDEFKFYTKIVHDEMLKRNYKPNELKYTSILKWKNRNFANDISNEHSLNLENL
                     YDDWHNKMYLKQCLYNLEEKATCGGIPINEWNILLCKYSKDYELWSGNIMF"
     gene            complement(2629..3084)
                     /gene="def"
                     /locus_tag="ACOIAF_002747"
     CDS             complement(2629..3084)
                     /gene="def"
                     /locus_tag="ACOIAF_002747"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HGZ2669750.1"
                     /translation="MAIRKIRTFDDEILRKKSKYVENVDNKIREILNDMAETMYNTPN
                     GGGLAACQVGVLKRLVVIDLGEGLIKLVNPEIIKQEGEQIVVEGCLSFPEVWGKLKRP
                     KKVTVQALNEYGEKIEIKGSGFMAKCLCHEIDHLNGIVFTDKIIEHVKL"
     gene            complement(3056..3319)
                     /locus_tag="ACOIAF_002748"
     CDS             complement(3056..3319)
                     /locus_tag="ACOIAF_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGZ2669751.1"
                     /translation="MKNKIKILREKLGLTQEQLGRLVGTSRQAINAIETGKNEPSIWL
                     AYDISRIFNEPIESVFLFEESERKSRAQISRGEYYGYKKDKNI"
     gene            complement(3416..4132)
                     /locus_tag="ACOIAF_002749"
     CDS             complement(3416..4132)
                     /locus_tag="ACOIAF_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2669752.1"
                     /translation="MIKIINAFKQEFEQIKRDAMLFIVCVSPILCGVFIKFGIPLIQN
                     ISLNKFYYQLNLEPYFLMFDLLLAFITPFMFFFASTMVILGEIDDSISRYLIVTPLGK
                     TGYLISRFGIPGILAFIITMILLIFFSLTKISLLLNLSISLLVLLQGVIISILVISLS
                     SNKLEGMVITKFSGVFMMGILAPFFILNKAQYILFFLPTFWISKAFKEDNYVYMFISI
                     VISLIWILLLFKKFSKKIVK"
     gene            complement(4129..4812)
                     /locus_tag="ACOIAF_002750"
     CDS             complement(4129..4812)
                     /locus_tag="ACOIAF_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2669753.1"
                     /translation="MRLKNLILGDIKFQFKYGFYFLYLFLSIIYICIINVFPTFMREK
                     IAIIMIYSDPAAMGLFFMGAIVLLEKSQRVLNSLAVSPVKVSEYILSKVISLGVISSI
                     VAMFIAITLNLDNIIISTIGTFFSSIIFSLLGLIIASKASSLNQFIVLSIPIEIICFI
                     PPILNVLLDTKSYANLYPFNICISLISGDKNFIMINILILISIIIIIYFITYYFICSS
                     WKKVGGVKL"
     gene            complement(4809..5654)
                     /locus_tag="ACOIAF_002751"
     CDS             complement(4809..5654)
                     /locus_tag="ACOIAF_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2669754.1"
                     /translation="MIKVDDLSFSYTDRDFLQNINFEVGKGEILGFLGPSGAGKSTLQ
                     KILIGMITNYGGSVIVNGVESKRHSNKFYENIGVDFEFPSLYEKLTAIENLKYFGSLY
                     SKKLLSIDELLKSVGLENESNKRVSEYSKGMKSRLNFIKALLHNPDILFLDEPTSGLD
                     PSNSKVMKDIILSEKSKGKTIILTTHNMLDATELCDRVAFIVNGKISALDTPHNLIMS
                     KGAIKVRYTYFDNGEKTSECFLNNTANDKNLNMLIEKNKLLSIHSSEPTLNDIFIEIT
                     GRNLQ"
     gene            complement(5768..6358)
                     /locus_tag="ACOIAF_002752"
     CDS             complement(5768..6358)
                     /locus_tag="ACOIAF_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HGZ2669755.1"
                     /translation="MPKSYSDKEREYIIKRLKEEARLCMEQYGIRKTTVDELVKRVKI
                     PKGTFYLFFQSKELLFFEVLRDIHDSIQKEILYEINRVDESITCEQLTDIFMKFYRMV
                     DSTSILNLMINGEFEILVRKLPDSIIEEHFRHDDFEIGEIISCIPNAKNKDIESFSGA
                     FRAVFLTMLYKREVGSNCFEDALRLMINGLVIQLME"
     gene            6647..6880
                     /locus_tag="ACOIAF_002753"
     CDS             6647..6880
                     /locus_tag="ACOIAF_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HGZ2669756.1"
                     /translation="MATTTKNRFYDYVQENLNKYVEVHSYFSSCPLYGEIIEADSLSI
                     TLCSRYIDESNPTLNETYTLYLPLSSIISIRTL"
     gene            complement(7111..9498)
                     /locus_tag="ACOIAF_002754"
     CDS             complement(7111..9498)
                     /locus_tag="ACOIAF_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp13"
                     /protein_id="HGZ2669757.1"
                     /translation="MKKFTSKKVTRFVALFLISILVMSTIPVSADNSNTLNQIKKAEY
                     SETYKQYLNDAKNGRTEKYNGIIPNPYQLEGTQIQSKGRTAPTSYDPRKLGLMTSIKN
                     QEDLGICWDFAAMATLESFLKLNNYGDYDLSEEHLRWWASDGEYGWSVDDMNGALNYE
                     AMGYLTSWSGPKLEKDIPYNGRVSKAQGAKKPTNMNTAPTVFNVTDAVCVSNDINSTK
                     NAILQYGAVTSGYYEDIKYQSDDQNSYYCPTKSFNTNHAISIVGWDDNYSKDNFNANI
                     RPSKNGAWLIKNSWGDYNSEGGYFWISYEDKTLMSDIDNFSIKGGKKPNDDEKMYQHD
                     YASIVPLISKKITAANVFDFNRGDETLKSVMFLTESIGAKYEVYYAPVVNGIPQENNM
                     KKLKEGTAQYSGYITVPIDSFDIPEGKGAIVVSIEGKNGESTIGSESNVPGYDTFKAK
                     ANLGESYIIDNTGKFFDINRDSNFYPCNFTIKAVTEKSSGESIPNESLIGSDRYETAI
                     KVSQNGFNSSENVVLVNGSSIVDALAATPFTSAINSPILLTQKEALNSKTKAEIQRLG
                     AKKVYLIGGENSISKEIEQQLKSLNISIERISGSDRYKTSLLLAQKLNGIKNVSQIAV
                     VNGVKGLADAISVGAAAAENNIPIILANEKSELQGADEFLNSLNIEKSYIIGGTASLS
                     NNLESKLKNPTRLSGSSRDETNSKIIDNFYKKDTLKNAFVVKNGIKNQNDLIDGLAVG
                     PLGAKTGSPVILVGDKLADSQKEVLKNKTLEKVTQVGGGANKNAFKELVKLKSAK"
     gene            complement(9690..10445)
                     /locus_tag="ACOIAF_002755"
     CDS             complement(9690..10445)
                     /locus_tag="ACOIAF_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HGZ2669758.1"
                     /translation="MFSIGVDSGSVATKGVLFDGEKIIKKIIIPTGWSPKSTSKQVYE
                     LLSSEIDKKDIKKVVGTGYGRGVMDFADKKVTEITCHTRGIYFLNKNIRTILDVGGQD
                     SKVINLDRDGNVFNFIMNDKCAAGTGRFLEITSNLLGSDIESIDTLAKGYEPVNISSM
                     CTVFAESEIVSLLAQNISTGEVAAGILKSIANKSTSMLARGEVIDEVAFTGGLAKSKE
                     LVKMIEEILGKKIFIAEDTQIIGALGAAVIGFR"
     gene            complement(10470..11621)
                     /locus_tag="ACOIAF_002756"
     CDS             complement(10470..11621)
                     /locus_tag="ACOIAF_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HGZ2669759.1"
                     /translation="MTNLPKQFDSFNEARQKGFINAKELKESGKKMVGVFCTFTPVEI
                     PMAAGATVVGVCGVSEEPIPDAERVLPRNLCPLIKSSYGHAITDTCPYFYFSDLLIGE
                     TTCDGKKKMYEELAKVKPTYVMHLPNTSKGEFAYKLWKDEMIRLKEEVEKSLGVTITE
                     EDIRTAIKDKNEERELLKEFYALGKLQPSALTGLELHNVLYQAGFKFDRAELKHSLRK
                     VIDDMKERYEKGECPVQKDKPRILITGSPIGGISEKIVKTLEDAGASVVAYELCGAIR
                     SNDLLVDEEIEDVYDALTQKYINIGCSCMMNNDNRIELLDRIIDEYNVDAVIDVVLQA
                     CHTFNIESYRIREFVTKEKNKPFMSLETDYSKSDTEQLRTRFEAFVEML"
     gene            complement(11837..12577)
                     /locus_tag="ACOIAF_002757"
     CDS             complement(11837..12577)
                     /locus_tag="ACOIAF_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HGZ2669760.1"
                     /translation="MKDYEDVVYIKLVKKELEEWKKNILKKPSIFDKASKGIQNKFNG
                     VLPDKYHEIITSSIKVMTKTVLFGYKYATKPPLRNMSLEEREKLVLEKAKTYKTTAMI
                     EGAGTGAGGILLGLSDFPLLLGIKVKFLYDVASIYGYDVKDYRERIYILNILQLAFSS
                     QQHINKTFKLMNNWDEYVNSLPEDINDFDWRTFQQEYRDYLDLAKFLQLMPGIGAFVG
                     FYVNGKLIDKLCETAVSAYRLRIRELKL"
     gene            complement(12649..13638)
                     /gene="add"
                     /locus_tag="ACOIAF_002758"
     CDS             complement(12649..13638)
                     /gene="add"
                     /locus_tag="ACOIAF_002758"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HGZ2669761.1"
                     /translation="MFENLPKIDLHCHLDGSVRVETMLDIAIKEKIDLPSNNMDEIKK
                     LAKVSFNCTSLDEYLEKFDLPLKVMQSKENLKRITFELLEDASRENVKYIEIRFAPLL
                     HTQKGMSVKNIIEGIIEGIREAESIYDIKGNLILGCMRTMTSKEALLVIEEGKSFVNK
                     GVVAVDLCGPEKEGFCKEYKDVFKLAREYGYKVTIHAGEAASGENVLDAINILKADRI
                     GHGVKIKDHKKAYNLVKDKKILLELCPTSNVQTKTVDSYEAHPFYTFYKDNLHVSINT
                     DNRTVSDINLSSELNVIFDTFKLGLEDYKIIYRNAVEASFADKETKEYLNSLI"
     gene            13898..>14470
                     /locus_tag="ACOIAF_002759"
     CDS             13898..>14470
                     /locus_tag="ACOIAF_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HGZ2669762.1"
                     /translation="MITLSFDIVQTLTLSIFFFLIGNLLKNKVNFLNNFCIPAPVIGG
                     LLFCFLNLFFKYFNIADISISGNLMPNFITFFFTTIGLEISINLIKKGGSVLFRYWIL
                     CGVLAFCQNILAITISKIIKLEPLLGLMCGNVSMEGGHGYSAAFGLTIENLGIEGAVG
                     VGLSAATIGLIMGGILGCPVAKFLINKYKLK"
BASE COUNT         4802 a         2432 c         1461 g         5775 t
ORIGIN      
        1 cccatagtca tactatcgtc agaatatgct tcacccttaa cattactaaa aaactcaaca
       61 gacttaaatc caatattttc aacttctaat aagaaacttt cttttgaaaa atagtgattc
      121 caaacataaa atacattttc atctttttcg gttattacaa gagtttgctc taatgtatta
      181 taatcattgt atttacaatt atcttgtaga cacatatatt tttcattact ccaaaatcct
      241 ccatcttcat ttatttccca atatttaatt tcctcaaaat tattgtattt atttattgta
      301 aatacatcta atattaattt tcctccaggt tttaaactat cataaatttt ttccattaat
      361 aaccttctat tttcagttga caaagctcca taatcacaat aaattaaggt agccaaatca
      421 aattcctcat tatagttcat atttaaataa ctttggaaca agtaatttat gtttaaatta
      481 gtctccttat ttctattttg tgcatagtta atagaacgtt ttgagaaatc aattccagtt
      541 accttataac ctttttgtgc aaatttctct gcatataaac ctggaccaca acctaaatca
      601 agtaatttag gataattagc tggatttgca actgtaacta tccaatttac agaatcctcg
      661 ataaagttaa aatttctact tgctccttca aaattagtgt ctaagtgtgc ttttaataat
      721 tgctttgaaa tatactcatc atcccaaaaa ttaatttcac tttgtttata aagctctggt
      781 ctttctaaat acatacataa tttgtttaac aaaacatacc actccttaaa atttattttt
      841 aaaataaaaa aaccacagag tttactctgt ggtttaactt caaaaagatg agtttaaata
      901 agagtaaaat agaaccaaat atttttaagc acaacaataa aactatagct ttactttgtt
      961 tgcttattaa atttgatttt attcactctt ttccagcaat acttatatca acgattttat
     1021 gatatcaaat atcaccgaac aacatcatcc tttcatattt ttattttatg aattaattat
     1081 atatttaaat tttatatttg tcaataaatt ttaattttaa taaattttaa tcatcatagt
     1141 aaaaaaaatt tttttatata aaactaatca aaaaaagata tctgtacatc tcctgagttt
     1201 tttaactttt ccttcattat tttagagtaa ctgcttgata gagaatactt atatctaagt
     1261 tcattaacca ttttatacaa ttgattttta tactccctat taagtgaacc tgcactatat
     1321 agtattgata atttatcaag taattcagga aactcttttt taacaaagtc aaaaaaaaca
     1381 cctctagtta taccccttaa gtataaagtc ccaggtaaaa cataatgtac attacattct
     1441 tttgcatgtc taaatagcga atttatatta tcaaaatcat ctgtaatata tggtataatt
     1501 ggcataatat gtagaccaac tgatgcgtta gtttttctaa attcttttaa cattttaaat
     1561 cgtctcattg aatccacacc atttggctca ataagccttt gtgtatcttc atttactgtg
     1621 gtaattgtag atgctacatt gatatatgta atcctagata gcttatcaat taaatcgtaa
     1681 tctcttaaaa ttaaatctga ctttgtagaa ataatagctg gtgtcttgta ttttattagc
     1741 aggtttaata tttctggcat aattttatag tcagcttcta ttggttgata actatctgta
     1801 actcctccta tattgattac ttctttcttc catttactgc ttttaagttg tctctcaagt
     1861 ttttctacta tatttgtttt tacataaatc tcttcaaaat aattattgga gttgatgtac
     1921 ttatgagagt aaatagcata acaatattta catccatgct cacatccacg ataaatattt
     1981 aaatcccatt tatatggcat actccttttt aatttattac aagctatttc acaatttatt
     2041 ggaatatatt ttattttatt atacaaataa tcacctcttt atatacaata atactataat
     2101 gttaatctat agtaattaaa acatgatatt tccagaccac aattcataat ctttactata
     2161 tttacaaagt aatatattcc attcatttat tggtattcct ccacatgttg ctttttcttc
     2221 taaattatat aagcattgct ttaaatacat tttattatgc caatcatcat ataaattttc
     2281 taaatttaaa ctatgttcat tagatatatc atttgcaaaa tttctatttt tccattttaa
     2341 tatacttgta tatttaagtt cattaggttt ataatttctt tttaacattt catcatgtac
     2401 aatttttgta taaaatttaa attcatctat actataattc aatactttat tcactaatag
     2461 atggtttgga gtaccttttt tatctataga accttttata gcaagtagct ctctccattg
     2521 acttactaat tgatttttag gtaatacatc aattaaatcc ttatgccata atctcataat
     2581 ttaactcctt actataaaat attttacaaa ttcaataacc aatctatctt acaattttac
     2641 atgttcaata attttatctg taaaaactat accatttaaa tggtctatct catgacaaag
     2701 acattttgcc ataaaacctg aacctttaat ttctattttc tcaccgtatt cgtttagagc
     2761 ttgcactgta actttttttg gtctttttaa tttaccccat acttcaggaa aacttaaaca
     2821 tccttcaaca acaatttgtt ccccttcttg tttaataatt tcaggattaa caagcttaat
     2881 aagtccttct cctaaatcta taacaactaa tcgctttaat actcctactt ggcatgcagc
     2941 taatcctcct ccatttggag tattgtacat ggtctctgcc atgtcattta atatttctct
     3001 tatcttatta tctacgtttt ctacatactt actctttttt cttaaaattt catcatcaaa
     3061 tgttcttatc tttcttatag ccataatatt ctcccctact tatctgtgct cttgattttc
     3121 tttcactttc ttcaaataaa aatacacttt caattggttc attaaaaatt cttgaaatat
     3181 catatgccaa ccatattgaa ggttcatttt taccagtttc tatagcattt atagcctgtc
     3241 tagatgttcc aactaatctc cctagttgtt cttgtgtcaa tcctaatttt tctcttaata
     3301 tctttatttt atttttcata tttgctccat tcatgtaagg ttaactttac atcttaaatg
     3361 taacattttt caacattact gtcaagtcaa ccttacattt atatattaaa ttttcttatt
     3421 taactatttt tttactaaac tttttaaata ataataaaat ccatattaat gatataacaa
     3481 ttgaaataaa catatagaca taattatctt ccttaaatgc cttcgatatc caaaaagttg
     3541 gtaagaagaa cagaatatat tgtgctttat ttaaaataaa aaatggtgct aaaataccca
     3601 tcataaaaac acctgagaat ttcgtaatta ccattccttc tagtttattt gatgataaag
     3661 aaatcactaa tattgatata ataactcctt gtaataaaac taataaagaa attgataagt
     3721 ttaacaagag agaaatttta gttagagaaa aaaagataag tagaatcata gtaataataa
     3781 atgctaagat acctggtatt ccaaatcgtg aaataagata accagtttta ccaagcggag
     3841 ttactattaa atatcttgaa atgctgtcat caatttctcc caatatgacc attgtagaag
     3901 caaaaaagaa cataaaaggt gtaataaaag ctaaaagtaa atcaaacatc aagaaatacg
     3961 gctctaaatt taattgataa taaaacttgt taagtgatat attctgtatt aatggtattc
     4021 caaattttat aaatactcca cataatattg gagaaacaca tactataaaa agcatagcat
     4081 ctctcttaat ttgttcaaac tcttgtttaa acgcatttat aatctttatc atagttttac
     4141 tccacctacc tttttccaac tgctacaaat aaaataataa gttataaaat aaattattat
     4201 tataattgat attaaaatta aaatattaat cattataaaa tttttatctc cactaataag
     4261 agaaatacat atattaaaag ggtacaaatt tgcatatgat tttgtgtcaa gaagtacatt
     4321 cagtattggt ggaataaagc atataatctc aataggaata cttaaaacaa taaactgatt
     4381 caaactagat gctttacttg caattatcaa tccaagcaaa gaaaatataa tagaagaaaa
     4441 aaaggtacct atagttgata ttataatatt atctaaattt aatgtaatag caataaacat
     4501 agctacaata ctagatatta ctcctaatga aattaccttt gataaaatat actcagatac
     4561 ttttactgga gatacagcta aggaatttaa aaccctttga cttttctcaa gaagtacaat
     4621 agctcccata aaaaacaatc ccattgctgc tgggtcagaa taaatcataa ttatagctat
     4681 tttttctctc ataaaagttg gaaatacatt gataatacaa atatatataa tacttaaaaa
     4741 aagatataaa aaataaaaac catacttaaa ttgaaacttt atatctccta aaataagatt
     4801 ttttagcctc attgtaaatt cctccctgtt atctcaataa aaatatcatt taatgttggt
     4861 tcactactat gtattgataa aagtttattt ttctcaataa gcatattcaa atttttatca
     4921 ttagcagtat tgttcagaaa acattcagaa gttttttctc cattatcaaa gtaagtatat
     4981 ctaactttaa tagctccttt tgacataata agattatgtg gtgtatctaa agcagatatt
     5041 ttaccattta caatgaacgc tactctgtca caaagctctg ttgcatctaa catgttatga
     5101 gttgttaaaa taatagtctt acctttcgat ttttctgata aaattatatc tttcataacc
     5161 ttgctatttg atgggtcaag accacttgtt ggttcatcta aaaataaaat atctggatta
     5221 tgtaataaag cttttataaa atttaatcga gatttcatac cttttgaata ctcagatacc
     5281 cttttatttg attcattttc tagtcctact gattttaata actcatcaat agatagtagt
     5341 tttttagaat atagagaccc aaaatatttt aaattttcta ttgcagtcaa cttttcatat
     5401 aaactaggaa attcaaaatc aactcctata ttttcataaa atttatttga gtgtctttta
     5461 ctctctactc catttacaat gacacttcct ccataattag ttatcattcc aattagaatc
     5521 ttttgtaatg tagattttcc tgcacctgat ggtcctaaaa atcctagtat ttcccctttt
     5581 cctacttcaa agttaatatt ttgtaaaaaa tctctatctg tataactaaa tgataaatca
     5641 tcaactttaa tcattcaata tcaccctttc aaatttttga ctattttgac tatatggtca
     5701 ataaaatata atttttttgt ttgttaataa tttttaataa aagttattag caaacaaaat
     5761 ttaaattcta ttccataagt tgtataacta atccgtttat cattaatctt aaagcatctt
     5821 caaaacaatt acttccaacc tcgcgcttat atagcatggt aagaaaaact gctctaaatg
     5881 ctccactaaa gctttctatg tctttatttt ttgcatttgg tatgcaagat ataatctctc
     5941 caatttcaaa gtcatcatgt ctaaagtgtt cttcaattat agaatctggt aactttctca
     6001 ccaatatctc aaattctcca tttatcatga gatttaaaat tgaagtacta tcaaccatcc
     6061 tatagaattt cataaatata tctgtcaatt gttcacatgt aatactttca tctactctat
     6121 ttatctcata taagatttct ttttgtatag agtcatgtat atctcttaat acctcaaaaa
     6181 agagcaattc ttttgattga aaaaatagat aaaaagttcc ttttggtatt tttacccgtt
     6241 ttacaagctc atcaacagta gtttttctaa taccatattg ctccatacac aatcttgctt
     6301 cttctttaag tctttttatt atatactctc tttctttatc agaatatgat ttaggcatct
     6361 ttttcctcca gtttgactaa aataatttat atagtcaata taatactttt tctatctaat
     6421 gtcaatagta ttattagtat atttaaaaat aagcttaaaa actttcgaaa attctgaatt
     6481 ttatatagca ttttttatta tctagtgtta taattaaaat ataaataata tattattcta
     6541 attttcatat ctatttgctt attttttaac ttagtttcta atagaagtaa tcatatttat
     6601 ctttttaaaa gtttcaactt taataaaaaa taaaggagtt gattttatgg ctactactac
     6661 aaaaaaccgt ttttacgatt atgttcaaga aaacttaaat aaatatgttg aagtacattc
     6721 ttactttagc agttgtcctc tatatggtga aattatagaa gcagatagct tatcaattac
     6781 actttgctca agatatatag atgaatctaa tccaacttta aatgaaactt acacattata
     6841 tttaccatta agttcaataa tatctattag aacactataa aaagttaatt aaatatgggt
     6901 tatttatata taaaatgtaa gattttctat atttctatat aaagtaaccc ttttttatgt
     6961 ttttaaactt atactacata aatttaaagc tgaaaataaa attttacaat cattgattat
     7021 cttatttatc aataatttaa agtaattatt ttcagctttt attaagataa gttgctaata
     7081 cacactaata tatgttaata cttaagtact ctatttagca ctttttaatt ttactaactc
     7141 tttaaatgca tttttatttg ctcctccacc aacttgagtt actttttcta aagtcttatt
     7201 tttaagaact tctttttggc tatcagctaa tttatcacca actaaaatta ctggtgaccc
     7261 agtttttgct cctaaaggac caactgccaa tccatctatt aaatcatttt gattcttgat
     7321 tccattttta actacaaatg cattttttaa agtatctttt ttataaaaat tatctattat
     7381 ctttgaatta gtttcgtctc tactactacc agataatcta gttggatttt ttaacttact
     7441 ttctagattg tttgataggg atgctgttcc tccaataata taagatttct ctatatttaa
     7501 tgaatttaga aattcatcag ctccttgcaa ttcgctcttt tcatttgcaa gtatgattgg
     7561 tatgttattt tctgctgctg cagcgcctac actaattgca tcagctaatc ctttcactcc
     7621 atttacaact gctatttgag atacattttt tataccattt aacttttgag ctaataataa
     7681 acttgtcttg tatctatcac ttcctgatat tctctctatc gatatattta agctttttaa
     7741 ttgttgttca atttctttac ttatagaatt ttctccacct attaggtata ctttcttagc
     7801 acctaatctc tgtatctcag ctttagtttt tgaatttaaa gcttcttttt gtgtcaataa
     7861 tattggtgaa tttatagcag atgtaaaagg agttgctgcc aatgcatcta ctattgaact
     7921 gccatttact aaaactacat tttctgaaga attgaaacca ttttgactaa ctttgatagc
     7981 agtttcatat ctatcactac caattaaaga ctcatttggg atactctcac cagaagactt
     8041 ttctgtaaca gcttttatag taaaattaca tggatagaag ttactatctc tatttatatc
     8101 aaagaatttt ccagtattat ctatgatata actttcacct aaattagctt ttgctttaaa
     8161 tgtatcatat cctggtacat ttgactcaga acctatagtt gactctccat ttttcccctc
     8221 tatgctgact acaattgcac cctttccttc tggtatatca aaagaatcaa ttggaactgt
     8281 tatgtatcct gaatattggg cagtaccttc ttttaatttt ttcatattgt tttcttgagg
     8341 tataccattt actactggag cataatatac ttcatactta gctcctatac tttcagttaa
     8401 gaacattaca gattttagag tttcatcgcc tctattgaaa tcaaatacat ttgcagctgt
     8461 tatctttttt gaaattaaag gtacaatact tgcatagtca tgttgataca tcttctcatc
     8521 atcatttggt tttttaccac ctttgattga aaaattatct atatcagaca taagtgtctt
     8581 gtcttcataa gatatccaga aatatccacc ctcagaatta taatctcccc agctattttt
     8641 tattaaccaa gcaccatttt ttgatggtct aatatttgca ttaaaattat ctttagaata
     8701 gttatcatcc caacctacaa ttgaaatagc atgatttgta ttaaaacttt ttgttggaca
     8761 ataatatgaa ttttggtcat cactttgata tttaatatct tcataataac cagatgttac
     8821 tgctccatac tgtaatatag cattttttgt agaatttatg tcattactta cacacacagc
     8881 atctgtcaca ttaaaaacag taggtgctgt attcatatta gttggttttt ttgctccttg
     8941 tgctttagat actctaccat tgtatgggat atctttttct aatttaggac ctgaccaaga
     9001 tgttaaatat cccatagctt cataatttaa agcaccattc atatcatcta cagaccatcc
     9061 atattcacca tctgaagccc accatcttaa gtgttcttct gataaatcat agtctccata
     9121 attatttaac tttaagaaac tttctaaggt tgccattgca gcaaaatccc aacagattcc
     9181 taaatcctct tgatttttta ttgatgtcat aagacctaat tttctagggt cataagatgt
     9241 tggagctgtt cttcctttac tttggatttg agtaccttcc aactgatatg gatttggtat
     9301 aatcccatta tatttttctg tacgtccatt tttagcatca tttagatatt gtttatacgt
     9361 ctcactgtat tctgcttttt ttatctgatt caatgtattg ctattgtcag ctgatacagg
     9421 aattgttgac attactaaaa tagatattaa aaacagcgca acaaaccttg ttactttttt
     9481 tgaagtaaat tttttcacgc tttctccccc ttattttgag ctaattttta aaattataat
     9541 gttatatatg actaaatatc aagtatttac aaatatacaa tcatttaaat caattatacc
     9601 aaagttttcc tttataaata aataaaataa aataaaaaac tgaatttatt tataataaat
     9661 tcagcttctc attttctttt tatatgtttt tatctaaatc ctattacagc agctcctaaa
     9721 gctcctatta tttgagtatc ttctgcaata aaaatttttt tacccagtat ttcttctatc
     9781 atttttacta gttctttact ttttgcaagc cctccagtaa atgcaacttc atctattacc
     9841 tcacctcttg ctaacattga ggtggattta tttgcaatag atttaagtat ccctgctgct
     9901 acctctccag tagaaatgtt ttgagctaga agacttacaa tttcagactc agcaaaaact
     9961 gtacacatac tagatatatt aactggttca tatcctttag ctaatgtatc tatactttca
    10021 atatcgcttc ctaaaaggtt agaagtaatc tctaaaaatc gaccagttcc tgcagcacac
    10081 ttatcattca ttataaagtt aaatacattt ccatctctat ctaaatttat aactttacta
    10141 tcttgaccac ctacatctaa aatagtcctt atgttcttgt ttaaaaagta aatacctcta
    10201 gtatgacaag ttatttctgt tactttctta tcagcaaaat ccattactcc ccttccataa
    10261 cctgtaccta ccaccttttt tatatctttt ttgtcaattt cactacttag caactcatag
    10321 acctgtttag atgtactttt agggctccat ccagttggta taattatttt cttaataatt
    10381 ttttcaccat caaatagcac accctttgtt gccactgacc ctgagtcaac tcctatacta
    10441 aacatagttc ctcctacttt tatttattcc tacaacatct ctacaaacgc ttcaaatcta
    10501 gttcttaatt gttcagtatc tgattttgaa taatcagttt caagagacat aaatggctta
    10561 tttttttctt ttgtaacaaa ttctcttatt ctataacttt ctatattaaa tgtatgacat
    10621 gcttgtaaaa ctacatctat tacagcatct acattatatt catctattat tctatctaat
    10681 aattcaatac gattgtcatt attcatcata caagaacaac ctatatttat atatttttgt
    10741 gttaaagcat catatacatc ttctatttct tcatcaacca ataaatcgtt tgatctaata
    10801 gctccacaaa gttcatatgc aacgactgat gcccctgcat cttctaaagt tttaactatt
    10861 ttttcgctta ttccaccaat aggagaacct gttatcaata ttcttggctt atctttttga
    10921 acaggacatt cacctttttc atatctttct ttcatatcat ctataacttt tcttaaagaa
    10981 tgtttaagtt ctgcacggtc aaatttaaaa cctgcttgat ataaaacatt atgtaactca
    11041 aggcctgtaa gtgcagatgg ttgtaattta ccaagtgcat aaaactcttt taaaagttct
    11101 ctttcttcat ttttatcttt tatagcagtt cttatatctt cttcagttat agttacacct
    11161 aaagatttct caacctcttc ttttaatctt atcatttcat ctttccaaag tttataggca
    11221 aattctcctt ttgaagtatt tggcaaatgc attacatatg ttggcttaac ttttgcaagt
    11281 tcttcataca tttttttctt accatcacaa gtggtttctc ctatcaacaa atcagaaaaa
    11341 tagaaataag gacaggtatc tgttattgca tgtccatagc ttgatttaat taaaggacaa
    11401 agatttcttg gaagcaccct ttctgcatca ggtattggct cttcactaac gccgcataca
    11461 cctactacag ttgctccagc tgccattgga atttcaactg gagtaaatgt acagaaaact
    11521 ccaaccatct ttttcccact ttccttaagt tcctttgcat ttataaatcc tttttgtcta
    11581 gcttcattga agctatcaaa ttgttttggc aaatttgtca tgaaaatatt cctcccaatt
    11641 acttctcttc ataattcaat ctcttcgtaa ttcaatttgt atcttaagca ataaaaacta
    11701 aatttttcca ttaaaataca aacctctaca ttagattcta tcatgcttta ccttttatcc
    11761 ataattgttt ttatcaatgc aaaattagat acatatagat atattgtata actaattttt
    11821 tatgttataa tttttcttat aacttaagct ctcgaatcct caatctataa gcagacacag
    11881 cagtttcaca taatttatca attaattttc cattcacata aaatcctaca aatgctccta
    11941 ttccaggcat tagttgtaaa aacttagcta aatctaaata atccctatac tcttgttgaa
    12001 atgttctcca atcaaaatca ttgatgtctt ctggtagact atttacatat tcatcccagt
    12061 tattcatcaa tttaaaagtt ttgtttatat gctgttgact agaaaaagcc aactgtaata
    12121 tatttaatat ataaatcctt tctctataat ctttaacatc atatccatat attgaagcta
    12181 catcatataa aaatttcact ttaataccta aaagcaaagg aaaatcagat aaacctaaga
    12241 ggattcctcc tgcccctgtt cctgcccctt caatcattgc tgtagtcttg taagttttag
    12301 ctttttctaa aactaatttt tctctttctt ctaatgacat atttctaagt ggtggctttg
    12361 tagcatattt atatccaaac agtacggttt ttgtcattac ttttatagaa gaagtaatta
    12421 tttcatgata tttatctggc aatactccat taaatttatt ctgtataccc tttgatgctt
    12481 tgtcaaatat agaaggtttc tttaatatat ttttcttcca ttcttcaagt tcttttttga
    12541 ctagtttaat ataaacaaca tcttcataat ctttcacatt ttttctccta atacaatgta
    12601 tttttgtcaa tttaatagct taatttaaag tttaaatatt tttatctatt aaattaaact
    12661 attcaaatac tctttagttt ctttatctgc aaaactagct tctactgcat ttctataaat
    12721 tattttatag tcttctaatc ctagtttaaa agtatcaaat ataacattta gttctgaact
    12781 taaatttata tctgatacag ttctattatc agtatttata cttacatgta aattatcttt
    12841 ataaaaagta taaaatggat gtgcttcata agaatctact gtcttagttt gtacattact
    12901 agttggacat aattcaagca atattttttt atctttaact aaattatacg cctttttatg
    12961 gtctttaatt tttaccccat gaccaatcct atccgccttt aaaatattta tagcatctaa
    13021 tacattttct ccacttgcag cttctcctgc atgtattgta actttatatc catattctct
    13081 tgctaattta aaaacatctt tatattcctt acaaaatccc tctttctctg gaccacacaa
    13141 gtctactgct acaacacctt tatttacaaa tgatttccct tcttcaatta ctaataatgc
    13201 ctcttttgag gtcattgttc tcatacaacc taatattaaa ttgcctttta tatcataaat
    13261 actttcagct tctctaattc cttcaattat accctcaatt atatttttaa cactcatacc
    13321 tttttgggtg tgaagtaatg gtgcaaatct aatttcaata tattttacat tttctctact
    13381 tgcatcttcc aaaagttcaa aagtaattct ttttaaattt tccttagatt gcattacttt
    13441 taagggtaaa tcaaattttt ctaaatattc atctagtgaa gtacaattaa aagaaacctt
    13501 tgctaatttt tttatttcat ccatattatt tgaaggtaaa tctattttct ctttaatagc
    13561 tatatcaagc atggtttcta ctctaacact tccatctaag tggcaatgta aatctatctt
    13621 aggtaaattt tcaaacattt tatcctccta ctgtacttat aaataatact atcaacagat
    13681 ttttaagtct tatttctatt atatcaatat actacagttt ttaattctaa aaaagtcaca
    13741 tctcattaaa atattttaat ttaatttatg attatctaat tatatctact gattcttttt
    13801 aatatacatt tattaatata catttgataa taaaatttca atacataaga atttaagtta
    13861 tataattaaa actttgaatt acaaaggagg acttattttg attaccttaa gttttgatat
    13921 agtgcaaacc ttgacactat ctatattctt ctttttaatt ggtaatttat tgaagaataa
    13981 agttaacttt ttaaataatt tctgtatacc tgcaccagta ataggaggtc tattattttg
    14041 ttttctaaat ttatttttta aatattttaa tatagcagat atatctataa gtggaaattt
    14101 aatgccaaat tttataacct tcttttttac aactatagga ctcgaaataa gcataaatct
    14161 tataaagaaa gggggtagtg tattatttag atattggatt ttgtgtggag tattggcatt
    14221 ttgtcaaaat atattagcta ttacaatatc aaagattata aaacttgaac ctcttttagg
    14281 gcttatgtgt ggaaatgtat ctatggaagg tggtcatggt tattctgctg catttggact
    14341 tacaatagaa aatctcggta tagaaggagc agttggggtt ggattatctg ctgcaacaat
    14401 aggacttatt atgggtggta tattgggatg tcctgtagct aaatttctaa taaataaata
    14461 taaattaaaa
//
LOCUS       DBGJFF010000094        14440 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.095, whole genome shotgun sequence.
ACCESSION   DBGJFF010000094 DBGJFF010000000
VERSION     DBGJFF010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14440
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.095"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            952..2310
                     /locus_tag="ACOIAF_002760"
     CDS             952..2310
                     /locus_tag="ACOIAF_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HGZ2669763.1"
                     /translation="MKAKSTSKFELDGIPPLKEAIPLGLQHLMAMVIGSCLPAILIAN
                     ASGLNHKMSTLLIQAALVFAGLSTLLQLYPIPLFKGFKLGGGIPVIMGATAMFIGAGV
                     SVASKYGLPVLFGSQIIAGIVIIIIGFGLKKIRFIFTPAVSGTIVACMGLGLFPIAIK
                     NLAGGMGNETFGNPINFIVGISVSLMILGLNKYGKGLFKDASILVSIIFGYILSLILG
                     IVNFSSIQEFTLVALPKPLAFGLDIRLEVVVMFSIIYLVEIADIMGACTLSAVGGLNR
                     QVTDEELSSAVLGNGVLSTIGALFSATPMGMFGQNSAIVSNTKVVSKFVLAIGGIGLF
                     LAGISPLLANLIRTIPPCVVGGATLVIFSTLTTSGLRLVSMDGFNQENSMILGLSMAS
                     GIGFMVAPQVLEKFPKFIETLLADSSVVSGAMVAIIIQALYMVKFPGNKSDKVEDKNK
                     SL"
     gene            2417..3211
                     /locus_tag="ACOIAF_002761"
     CDS             2417..3211
                     /locus_tag="ACOIAF_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HGZ2669764.1"
                     /translation="MVTIKEYSVPQSLEEAYKILISKKNNVILGGCGFIKLSNKNIGT
                     AIDLKDINLNYIKEDEKNILIGADTSLRSLEIDKTIKNYCSGILSSAVSNIVGVQFRQ
                     GARIGASVFSKYGFSDLIPALLVVGAKVRLYNKGILELSEFLDSELSRDILVEVILPK
                     KDAIAVFDSIRKCTGDFSVLNSAMLKENDTYKIAIGARPQKARLALEASNILNKEKDI
                     DKASIVASKELTYGSNMRASKEYRKDMASALVKRMYNAIEEGMYND"
     gene            3204..5510
                     /locus_tag="ACOIAF_002762"
     CDS             3204..5510
                     /locus_tag="ACOIAF_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HGZ2669765.1"
                     /translation="MINKGIRKIDSTAIVTGKPLYTEDLIMHKDVLTLKLLRSPHAHA
                     KIKNIDTSKAEKIPGVVGIYTYKDVPQVRYCVPGQTEPEASPYDRVLLEDTVRFIGDE
                     VALVAAEDEKTALKALKMIKVDYEVLKPVFDAREAEGNEVVIHPEKDIVEPYPFGLDA
                     KNNIVSKEFFEFGDVEAKLKESDFVVESSYKTQSQSHCMMETHRSYSYKDINDRIVVV
                     SSLQSPHNVKRILRKVLGIPSSKIRVIKPRIGGGFGGKNIAATDIFTAFVTMKTGRPA
                     KFVFTRSETFVGSNSRHEMFFDVKVGADKEGNLKAIELKSLNNTGAYGGNGVSVSSES
                     GHNTLPIYNDIDAVRFNARTVYTNRLPAGALRGYGATQGIFAIDSAIDELAHKIGMDP
                     LEFRIKNIIDRYTNGKAAEDEILSCELRQCIEKGKKLINWDDKYPCKDIGNNKVRAVG
                     MAVANHGSGIPRVDMATVTIRMDEDGTYKLLSGLADLGQGADTIQAQIAAEALNTTVD
                     RISIYTGDSDLCPYDTGAFASCTTTVTGNATIKTANKLKDILLSVAESKLGFKKEDLE
                     LGKDRVFVKNDNSKFVLLEELGRESVGGIGFASDDVPVVLEATESFGMLKKIKPFIAG
                     FAEIELDKNTGEYKVINFACVPDCGTVINPTLARIQVEGGVMMGIGFAMYEDPVFDKD
                     GRLLTDSFLQYKVPTKKEVGNILVDFAENFDPGGPYGAKSLGEIVIHTPAPAIANAIF
                     NATGVRLRELPMTFEKVYMGMKNNNKQL"
     gene            5649..6926
                     /locus_tag="ACOIAF_002763"
     CDS             5649..6926
                     /locus_tag="ACOIAF_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HGZ2669766.1"
                     /translation="MSNRIKNIHYGTQRQQLKNLTKKEYQALREMCFLAKNLYNVGTY
                     NVRQHYFNQGEHLNYEANYHVCKENENYKLLNSNIAQQILKEVDSSFRSFFGLIKLAK
                     EGKYDFRGIKSPKYLDKEGFFSIIIGQIRIKEDGILNVPMSPAFKKLYGKVSIKVPQN
                     ILEKKIKEIRIIPKHKARFFEIQYCYEIPKSEEVSNQKNALAIDLGLENLCTCVTNLG
                     DSFIIDGKRLKSVNQWANKQNDKIEALKLKKNITAITRKQFKVWNKRNNQVKDYVNKT
                     CFYIIDYCVKNNIGNLIVGYNAKLEKSDNMERKIYRNFIRIPFGEIKGKLEYLCKIKN
                     INFIRQEESYTSKSDFFADDFLPDINVDNVKKYDFKGERITRGQYKSSVGIIINADVN
                     ASLNILKKSQVVNLSNLQNNPQILKQPQRVRIS"
     gene            7109..7831
                     /gene="yqeC"
                     /locus_tag="ACOIAF_002764"
     CDS             7109..7831
                     /gene="yqeC"
                     /locus_tag="ACOIAF_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HGZ2669767.1"
                     /translation="MELSNIIEKNEIITVVGAGGKTSFINYFANFYRDKLKVLLTTTT
                     KIYVPNDYDNIIITIDGTVIPSICHGITVCGSYINNENKLVSIDSSILDEIVDQFDLV
                     LIEGDGSKRKKLKGWNAKEPVVYHKTTKTIGILDITSFGMNINEENIHRVEIFKKIAN
                     LDTSSINSSSTVSIENLKNIVLNPNGLFKNYSGKRVLFINKVENEKYKNLAIKLIENI
                     KEYESEIEIFYGSVKQKFCVRY"
     gene            7938..8507
                     /gene="mocA"
                     /locus_tag="ACOIAF_002765"
     CDS             7938..8507
                     /gene="mocA"
                     /locus_tag="ACOIAF_002765"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HGZ2669768.1"
                     /translation="MINAVIMASGFARRMGANKLLLNYKGCSIIEHVFREVSKIDFHQ
                     VVVVSQYEEVLLLCNKYGFEYIDNKNANIGQSESIKLGVLNSSKCDGYMFFVGDQPFI
                     DNLYIDKIINTFKLDRDFIVIPRYKDMCGNPVIFPYSKKEELLALKEDEKGKTILKNS
                     SKIKYVEVPKEMLFDIDTKMDYERIGGKL"
     gene            8504..9523
                     /locus_tag="ACOIAF_002766"
     CDS             8504..9523
                     /locus_tag="ACOIAF_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HGZ2669769.1"
                     /translation="MKLIKTIDAVGQVLCHDITQIIPGEFKGRKFKKGHIVKEEDIPV
                     LLSLGKDNLYVWEKSEGMVHENEGALFLKELTAGENLDFSEIKEGKIDFIAACDGLLK
                     IDVDALFDLNCVGEIMMATLHNNFIVNKGLKVAGTRVIPLVIDEKKLEDAKKVVGNRK
                     IVNVVPFKPKKVGIVTTGNEVFYSRIVDKFGPVIEEKVKGFGCEVIGQTICPDDKEII
                     KSAIKEFISQGAELICCTGGMSVDPDDVTPTAIKETGADLVTYGSPILPGAMFLLAYY
                     GEVPIMGIPGCAMYHKTTVFDIVLSRVLIDEKLDKYDIARYGHGGLCMNCDVCTYPAC
                     NFAKI"
     gene            9861..11486
                     /locus_tag="ACOIAF_002767"
     CDS             9861..11486
                     /locus_tag="ACOIAF_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HGZ2669770.1"
                     /translation="MLQVTGVGLRFGDKELFKDVNLKFTKGNCYGIIGANGAGKSTFL
                     KILSGEIEPNTGSISITDKERMSVLKQDHFEYEEETVLNVVIRGHERLWNIMKEKDAL
                     YMKEDFNEEDGIKAAELEGEFAELDGWDAETNAEKILMGLGITKDMHYKQMKELVGGE
                     KVKVLLAQSLFGKPEILLMDEPTNHLDFKSINWLNNFIMDLEESIVIIVSHDRHFLNQ
                     ICTNIVDVDFGKIQMYVGNYDFWYESSQLALQLAKDQNKKTEEKIAQLKEFIARFSSN
                     ASKAKQATSRKKQLEKLEVEDIQPSRRRYPYVGFTPAREIGNEVLEVHNLTKTIDGVK
                     VLDNVSFRLDRDDKVVFMGDEIATTALLNIVMGELEPDSGEYKWGVTTSQDYLPKNHN
                     KFFDGVEYSLVDWLRQFSEEKSESFIRGFLGRMLFSGEEALKEAQVISGGEKVRCMLS
                     KLMLSNANVLVLDDPTNHLDLESITSVNKGLEKFPGVLLFTSHDHEFISTIANRIIEI
                     TPNGIMDRKMDFDEYLESKEIQDQLAKMYGKDK"
     gene            11685..12161
                     /locus_tag="ACOIAF_002768"
     CDS             11685..12161
                     /locus_tag="ACOIAF_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669771.1"
                     /translation="MVKKRLVAVVLSLAMISVGNFSYAHSGGHNSSGGHHNKNKSELT
                     RYYCGGHSAHQHNKGFCPYASTKENSGVTISSVHNEKNHKNYFAEKGYNKGYEDGCKG
                     LYNLSVTEAKSDNLYEKEILKSSYEKGYLIGYNEYKAKKVDDVMKKMLDIPMLASI"
     gene            complement(12369..13481)
                     /locus_tag="ACOIAF_002769"
     CDS             complement(12369..13481)
                     /locus_tag="ACOIAF_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGZ2669772.1"
                     /translation="MNAFIRKRNKNYVVYLEFEDEKTGKRKQKNMGTFDKKREASKRL
                     NEVKESLYNDGFLVPSEITVSEFLLDFLNKYSENISLATYNNYVSICKNYINPSIGKY
                     KIQDLHPVHIQNYIDKLSYKLNPQSIKIHINILKLAIKRAYRLKLIKENVMDSIEAPR
                     YKKFKNEIYDREQMIKLLDLAKNTEMELPINLAIGLGLRISEVLGLTWDNIDFEENTI
                     TVNKITSRINGSVILKEPKTESSVRKISSPKELMSLLKEYKIKQNKNLLKSSIRNNNN
                     LLFFNKKCEPIAEDVMSKKFKRFLEKNELPHIRFHDLRHSHVTLLINSKVPIKVISER
                     VGHSNISTTLSVYSHVLKEMDKEASDKISESLFNAN"
     gene            13822..14199
                     /locus_tag="ACOIAF_002770"
     CDS             13822..14199
                     /locus_tag="ACOIAF_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HGZ2669773.1"
                     /translation="MTITKIPPAELKVMKFIWSSDFTVASKDVIEAMEKLYNWKQTTT
                     LTLLSRLVNKQFLSARKIDRHTHYTVLIGEEEYLHFETKTFLDNMHGSSIESFMKSLF
                     KKGVNEEELNSVEKWVKDIKKDE"
BASE COUNT         5646 a         1489 c         2558 g         4747 t
ORIGIN      
        1 ttgtatattt tatgttgcag atacaaatat agtaaaggag agtacattgg tacaactgag
       61 atacatagct tattaagttt ttctgtctta tgagaattat attataaaat attcataaat
      121 attttgagtt taaattaaat aattatttta tgcttagatt aattgatatt taattaattt
      181 aataacaaaa acagtgaaag agtaaataat attatttaca gggggtaaag caaagtatgt
      241 taaataaatt gtacttttgg ccccgtacat agagaaatct atgtaggaca caactttaaa
      301 accagtaaat cctaaagcct tgcaactaca ataaaagata gaaagaaact tttatgaatg
      361 tggtgaaaac cagaaacaat agtgaggatg gtatatggtg agagcctaag tacttaaaaa
      421 tggacatttg gtcgccaagc ctagaaatag gaaggtcaaa cgactatctc gagagagagt
      481 acactataag cgattgatag tggaaacggg ttgctcttag caggtaatgc tgaagatgaa
      541 gaaatagtct acgctcatat gaaagtatga ggtcactgga tttgaagtga catctataaa
      601 gtagcgttta taggtaaatg tttaaaactt gaaacatgta aatacttatg aaaacttata
      661 acttataatg attaagattg aaagatttaa attgaattaa atttactata aaaaattaaa
      721 aattcatagc tataatactc ttatatttgt aaagcatggt atataatgta tacaaaatta
      781 aagaatacgt gtatttgtta ataattaaat ttattgcatc tgttttttaa gactaaaaac
      841 ttaaatatat gttggagtat agttgatatt tatgaacatc attaatttaa tatattagat
      901 ttttaagtat ttatataatt caataagttt tatgtaacgg gagtttttat tatgaaagca
      961 aaaagtacat caaaatttga attagacgga ataccaccac taaaggaagc aataccttta
     1021 gggttacaac acttaatggc aatggtaata ggtagttgtt taccagcgat attgattgca
     1081 aatgcatctg gacttaatca taagatgtca acattattga ttcaagccgc attagttttt
     1141 gcaggattat caactttatt acaattatat ccaatacctt tgtttaaagg ttttaaatta
     1201 ggaggaggaa taccagttat aatgggagct acagctatgt ttataggagc tggggtatct
     1261 gttgcaagta agtatggact accagtatta tttggctctc aaataatagc tggtatagta
     1321 ataattataa ttggttttgg tcttaaaaaa atacgattta tatttactcc agctgtatca
     1381 ggaacaatag ttgcatgtat gggtcttgga ctttttccaa tagctataaa aaacttagct
     1441 gggggtatgg gcaatgagac ttttgggaat ccaattaatt ttatagttgg aatatctgta
     1501 tctcttatga tattaggatt aaataagtat ggaaaaggat tatttaaaga tgcatcaata
     1561 ttagttagca taatttttgg atacatttta tcattaattt tgggtatagt aaatttttct
     1621 tcaattcagg agtttacttt ggttgcactt ccaaaaccac ttgcttttgg gctagatata
     1681 agacttgaag ttgttgtaat gttttcaata atatatttag ttgagatagc tgatataatg
     1741 ggtgcttgta cactttctgc ggtaggaggg cttaatagac aagtaacaga tgaagaactt
     1801 tcttctgctg ttttaggaaa tggagtactc tctacaatag gtgcattatt ttctgcaact
     1861 ccaatgggga tgtttggaca aaattctgca atcgtttcca atacaaaggt tgtaagtaaa
     1921 tttgtactag ctattggagg aattggatta tttttggcag gaatatctcc tttattagct
     1981 aatttaataa gaacaattcc tccctgtgtg gttggaggag caacactcgt tatattttca
     2041 actcttacaa catctggttt aagacttgtt agtatggatg gatttaatca agaaaatagc
     2101 atgatattag gtctttctat ggcttctggt attggattca tggtagctcc tcaagttctt
     2161 gaaaaatttc caaaatttat agagacatta cttgcagatt caagtgtagt ttcaggagca
     2221 atggtagcca taataattca agctttatat atggtaaaat ttcctggaaa taaaagtgat
     2281 aaggttgagg ataaaaataa aagtttataa tgtaaaggtt taatatatgt gaattaaata
     2341 tactacaatt ataaatataa ataaaagatt tattaaaaac ttttgtgttt taataagtta
     2401 atgatggggg gataagatgg ttactataaa ggaatattca gttccgcaga gtttagaaga
     2461 agcatataaa attttaattt ctaaaaaaaa taatgttatt ttaggtggat gtggatttat
     2521 aaaattaagt aataaaaata taggaacagc gatagattta aaagatataa atctaaatta
     2581 tataaaagag gatgaaaaaa atattttgat aggtgcagat acttcactta gaagtttaga
     2641 aattgataaa actataaaaa attactgtag tggaatactt tcaagtgctg tttctaatat
     2701 agttggtgtt caatttagac aaggggctag aattggtgca agtgtatttt caaagtatgg
     2761 cttttctgac ttaataccag cacttttggt tgttggagcg aaggtgagat tatacaataa
     2821 aggaatacta gagttaagtg agtttttgga tagtgaatta agtagggata tattggtcga
     2881 agtaatacta ccaaaaaaag atgcaatagc tgtttttgac tcaataagaa aatgcacagg
     2941 agatttttct gtattaaatt cagcaatgct aaaagaaaat gatacttaca aaatagcaat
     3001 tggggcaaga cctcaaaaag ctagattagc acttgaagcg agtaatatat taaataaaga
     3061 aaaagatata gataaggcat ctattgttgc tagtaaggaa cttacttatg gaagtaatat
     3121 gagagctagt aaagagtaca gaaaggatat ggcaagtgca ttagttaaaa ggatgtataa
     3181 tgctattgag gagggaatgt ataatgatta ataaggggat aagaaagata gatagtacag
     3241 caattgtcac aggaaaacct ctatatactg aagacctcat tatgcataaa gatgttttga
     3301 cattaaaact acttagaagt ccacatgctc atgctaagat aaagaatata gatacatcaa
     3361 aagccgaaaa aattccaggg gttgtgggta tttatacgta taaagatgta ccacaggtca
     3421 gatattgtgt tccaggtcaa acagaaccag aagcatcacc ttatgataga gttttacttg
     3481 aagatacagt tagatttatt ggagatgaag tagctttagt ggctgctgaa gatgagaaaa
     3541 cagcacttaa agccttaaaa atgataaagg tagattatga ggttttaaag cctgtgtttg
     3601 atgcaagaga agcagaagga aatgaagttg ttatacatcc agaaaaagac atagtagagc
     3661 catacccttt tggattggat gcaaaaaata atatagtatc aaaagaattt tttgaatttg
     3721 gagatgttga agctaagctt aaagaaagtg attttgtggt tgaatcttca tacaaaaccc
     3781 aaagtcaatc gcattgcatg atggaaactc ataggtcata ttcatataaa gatataaatg
     3841 atagaattgt tgtagtttca tcattacaat caccacacaa tgtaaagaga atacttagaa
     3901 aagtattggg tataccgtct tcaaaaataa gagtaattaa acctagaata ggtggtggat
     3961 ttggaggaaa gaatatagca gcaacagata tatttactgc atttgttact atgaaaactg
     4021 gtagaccagc aaaatttgta tttacaagaa gtgaaacttt tgtaggttct aatagtagac
     4081 atgaaatgtt ttttgatgtt aaagttggtg cagataaaga aggaaattta aaagcaatag
     4141 agctaaagtc attaaacaat acaggtgctt atggtgggaa tggtgtctct gtttcttccg
     4201 aatctggaca caatacctta ccaatatata atgatattga tgctgttaga tttaatgcta
     4261 gaacagtata tactaatagg ttaccagcag gagcacttag agggtatggt gctacacagg
     4321 gaatatttgc aatagatagt gctattgatg aattggcaca taagattgga atggacccac
     4381 ttgaatttag aattaaaaat ataatagata ggtataccaa tggaaaagca gctgaagacg
     4441 aaatattaag ttgtgagctg agacaatgta tagaaaaggg caagaaatta attaattggg
     4501 atgataaata tccatgtaaa gatataggaa acaacaaagt aagagcagta ggtatggcag
     4561 tagcaaatca tggttctggt ataccgcgtg tagatatggc aacagtcact ataagaatgg
     4621 atgaagatgg cacatataaa ctattatctg gtcttgctga cttaggtcaa ggggctgata
     4681 caattcaagc tcaaattgct gcggaagctt taaatactac tgtagataga atttctattt
     4741 atacaggtga ttcagactta tgtccctatg atacaggagc atttgcttct tgtacgacaa
     4801 cggttacagg aaatgcaaca ataaagacag caaataaatt aaaagatata ttactttcag
     4861 ttgcagagtc aaagctagga tttaaaaaag aagatttaga gttgggaaaa gacagggttt
     4921 ttgttaaaaa tgataatagc aagtttgttc tattagagga attgggtaga gaatctgttg
     4981 gaggaatagg atttgctagt gatgatgtac cagtagtttt ggaagcaaca gaatcttttg
     5041 gcatgttgaa aaaaataaaa ccatttatag caggatttgc agagatagaa ttggataaaa
     5101 atacaggcga atataaagtc ataaattttg catgtgtacc tgattgtgga acagttataa
     5161 atccaacttt agctagaata caagtagaag gtggtgtgat gatgggtata gggtttgcaa
     5221 tgtatgaaga ccctgtattt gataaagatg ggagattatt aacagatagt ttcctacagt
     5281 ataaagttcc tacaaaaaaa gaagttggaa atatattggt agattttgct gagaatttcg
     5341 acccaggagg tccttatggt gctaaatcat taggagaaat agttatacat acaccagcac
     5401 cagctatagc aaatgcaata tttaatgcta caggagttcg tttaagagag cttcctatga
     5461 cttttgaaaa agtttatatg ggtatgaaaa ataataataa acaattataa taaataataa
     5521 tgtgtataaa agcaagtaaa tttaaattat tttgtaaaat aaagtaatta aatggacatt
     5581 atatatggtg ttttgtaaaa ataccgtata taatgtctag tagagtatat tctaaaggag
     5641 gtgagttcat gtcaaacaga ataaaaaata tacactatgg aactcagaga caacaattaa
     5701 aaaacttaac taaaaaagaa tatcaagctc tacgagagat gtgcttctta gctaagaact
     5761 tatataatgt tggtacatat aatgtaagac agcattattt caatcaagga gaacatctta
     5821 actatgaagc taattatcat gtatgcaaag aaaatgagaa ctataagctt ctcaatagta
     5881 atatagcgca acaaatatta aaagaagtag acagttcgtt taggtcattt tttgggttaa
     5941 ttaaattagc caaagaagga aaatatgact ttagagggat aaaatcacct aaatatttag
     6001 ataaagaagg attttttagc ataataatag gtcaaataag aatcaaagaa gatgggattt
     6061 taaatgtacc tatgtctcca gcatttaaaa aactatatgg gaaagtaagt ataaaagttc
     6121 cacaaaatat actagagaaa aaaattaaag aaataagaat aatacctaaa cataaagcta
     6181 ggttctttga aattcagtat tgttatgaaa taccaaaatc agaggaagtt tcaaatcaaa
     6241 aaaatgcact agctatagac ttgggtttag aaaatttatg tacatgtgtt accaatttag
     6301 gtgatagttt tatcatagat ggaaaaaggc taaaatctgt taatcagtgg gcaaacaaac
     6361 agaatgataa aatagaagcc ttaaaattaa aaaagaatat aactgcaata acaagaaaac
     6421 aatttaaagt ttggaataag agaaataatc aagttaaaga ctatgtaaat aaaacctgtt
     6481 tttatataat tgattattgt gtaaaaaata atattggaaa tttaattgta ggatataatg
     6541 ctaagttaga aaaaagtgat aatatggaga gaaaaatata tagaaatttt ataagaatcc
     6601 catttggaga gataaaagga aaattagaat atctgtgtaa aataaaaaat atcaatttta
     6661 ttaggcaaga agaaagctat actagtaaat cagatttttt tgctgatgat tttctacctg
     6721 atatcaatgt ggacaatgtt aaaaaatatg actttaaagg ggaaagaata acgcgtggac
     6781 agtacaaatc cagtgttgga atcattataa atgcagatgt aaatgcgagt cttaatatac
     6841 taaaaaaatc acaagtagtt aatttatcta atttacagaa taatcctcaa atattaaagc
     6901 aaccacagag agtaagaata tcttaattat atttaaaaga taaactgata gcgtaagctt
     6961 acagcttagc ggcgaattgg atccgccttg ttgtttacaa cagaatctat ttactttcga
     7021 gtttatggaa tttcaaaaat cacttttact aaaaaggtct aactataata attaagagat
     7081 tagaaaactg gttataagag gtgaaagtgt ggaactatca aatataatag aaaagaatga
     7141 aataataact gtagttggag caggaggaaa aacttcgttt ataaactatt ttgcaaattt
     7201 ttatagagat aaattgaaag ttcttcttac aacaacaact aaaatatatg taccaaacga
     7261 ttatgacaat ataattatta caatagatgg aactgttatt ccatctattt gtcatggaat
     7321 aacagtatgt ggtagttata taaataatga aaataaatta gtaagtattg attcctctat
     7381 tttggatgaa attgtagacc aatttgattt agttttaata gagggagatg gctcaaagag
     7441 aaaaaaactt aaaggttgga atgctaaaga accagtagta tatcataaaa ctacaaaaac
     7501 catagggata ttagatataa catcatttgg aatgaatatt aatgaagaga atatacatag
     7561 agtagaaata tttaaaaaaa tagcaaattt agatacaagt agtattaata gtagttctac
     7621 agtaagtatt gaaaacttaa aaaatatagt attaaatcca aatggattat ttaaaaatta
     7681 ttctggaaaa agagtattat ttattaataa agttgaaaat gaaaaatata aaaatttagc
     7741 tataaaatta atagaaaata ttaaagaata tgaaagtgaa atagagatat tttatggaag
     7801 tgtaaaacaa aaattttgtg taagatattg aagtattaaa atagtttata tgatattata
     7861 tatatagata atatcaatag agatattata aaaatcatag aactatcaat atattataaa
     7921 taccaaggat gatatatatg ataaatgcag tgataatggc atcaggcttt gccagaagaa
     7981 tgggagcaaa taaactttta ttaaattata aaggctgttc tataatagaa catgtattta
     8041 gagaagttag caagattgat tttcatcaag ttgtagtagt aagtcaatat gaagaggttt
     8101 tgttattgtg taataaatat ggttttgaat atatagataa taaaaatgca aatataggtc
     8161 aaagtgaatc tataaaatta ggagttttaa atagctcaaa atgtgatggt tatatgttct
     8221 ttgtaggaga ccagccattt attgataact tatatataga taaaattata aatactttta
     8281 aattagatag agattttata gtaattccaa gatacaaaga tatgtgtgga aatccagtca
     8341 tattcccata tagtaagaaa gaagaattgt tggcacttaa agaagatgaa aaaggtaaaa
     8401 ccattttaaa aaattcatca aaaataaagt atgtagaagt tcctaaagaa atgttatttg
     8461 atatcgatac aaaaatggat tatgagagaa ttgggggtaa attatgaagc taataaaaac
     8521 tatagatgct gttggacaag ttttatgtca cgacataact caaataatac ctggagaatt
     8581 taaaggaaga aaatttaaaa agggccacat agtaaaagaa gaagatattc cagtgttatt
     8641 atctcttggt aaagataatt tgtatgtatg ggaaaagtca gaaggaatgg ttcatgaaaa
     8701 tgaaggagca ttgtttttaa aagaactaac tgctggagaa aatttagatt ttagtgaaat
     8761 aaaagaaggg aaaatagatt ttatagcagc atgtgatggt ctattaaaga tagatgttga
     8821 tgcacttttt gatttgaatt gtgttggaga aataatgatg gctactcttc ataataattt
     8881 tatagtaaat aaaggtttaa aagtagctgg aacaagagtg atacctttag ttatagatga
     8941 gaaaaaatta gaagatgcaa aaaaagtagt tggtaataga aaaatagtaa atgtagtacc
     9001 ttttaaacct aagaaagtag gaattgttac tacaggaaat gaagtgtttt atagcagaat
     9061 tgttgataaa tttggaccag taatagaaga aaaagtaaaa ggctttggtt gtgaagttat
     9121 aggacagact atatgcccag atgataaaga gattataaag agtgcaataa aagaatttat
     9181 aagtcaagga gcagaactta tctgttgtac tggtgggatg agtgtagacc cagatgatgt
     9241 tacgccaaca gctataaaag aaactggagc tgatttagtg acatatggtt caccaatatt
     9301 accaggagct atgtttttac ttgcttatta tggagaagtt ccaattatgg gtataccagg
     9361 atgcgcaatg tatcacaaaa ctacagtatt tgatatagtt ttatctaggg ttttaattga
     9421 tgaaaagctt gataagtatg atatagctag atatggtcat ggtggccttt gtatgaactg
     9481 tgatgtatgt acttatcctg catgtaattt tgctaaaata taaagttatt aaaaaagagt
     9541 atttacaaat taagtactct ttttaaatat gaaacaaaac agctagaatg atgttttaaa
     9601 aaaagcatat ttatttagaa attataataa cttattttca atgtgtaaaa gaatgaataa
     9661 agttgcaatt tgatttcttt ataagtataa tataagagat tgttaatttt tataaattta
     9721 caatttttag tattatttta ataaatattt cacaaattta gtataaaaaa tatactattt
     9781 acgtgataat atataataaa aatgttatca aagttttatg ataaaataaa ttatgagcaa
     9841 aaagaaagga aaatatatta atgttacaag ttacaggtgt tggacttaga tttggtgata
     9901 aggaactatt taaagatgtt aacttaaaat tcactaaagg taattgctat ggaataatag
     9961 gagctaatgg tgcgggaaaa tctacttttt taaaaatatt atcaggagaa atagagccta
    10021 atacaggtag tatatctata actgataaag aaagaatgtc agttttaaaa caggaccact
    10081 ttgaatatga agaagaaact gttttaaatg ttgttataag aggtcatgag agactttgga
    10141 acataatgaa agaaaaagat gcattgtata tgaaggaaga ctttaatgaa gaagatggga
    10201 taaaagctgc cgaacttgaa ggtgagtttg cagagcttga cggatgggat gcagaaacta
    10261 atgctgagaa aatacttatg ggtcttggca taacgaaaga tatgcattac aaacaaatga
    10321 aggaactagt tggtggggaa aaggttaagg tattgttagc acaatcactt tttggtaagc
    10381 ctgaaatact attaatggac gaacctacaa accacttaga ttttaaatca ataaactggt
    10441 taaataattt tataatggat ttagaagaat ctatagttat aatagtatca catgatagac
    10501 atttcctaaa tcaaatatgt actaatatag ttgacgtaga ttttggtaaa atacaaatgt
    10561 acgttggaaa ctatgatttc tggtatgagt caagtcaatt ggctttacaa ttggctaagg
    10621 accaaaataa aaagactgaa gaaaagatag ctcaattaaa ggaatttata gctagattta
    10681 gttctaatgc ttcaaaggct aaacaagcaa catcaagaaa gaaacagtta gagaaattag
    10741 aagttgaaga tatacaacca tcaagaagaa gatatccata tgtaggattt acacctgcaa
    10801 gagaaattgg gaatgaagtt ttagaagtac acaatttaac taaaactata gatggggtta
    10861 aggttcttga taatgtgtca tttagattgg atagagatga taaagtagta tttatgggag
    10921 atgaaatagc tactactgca cttttaaaca tagttatggg agaacttgaa ccagacagtg
    10981 gagagtataa atggggagta actacttctc aagattatct tccaaaaaat cataataagt
    11041 tttttgatgg agtagaatat tctttggttg actggcttag acagttctct gaagaaaaaa
    11101 gtgaaagttt tataagagga tttttaggta gaatgttatt ctcaggtgaa gaagcattaa
    11161 aagaagctca agttatatct ggaggagaaa aggttagatg tatgcttagt aagctaatgc
    11221 tttcaaatgc aaatgtactt gtattagatg accctactaa ccacttagac ctagaaagta
    11281 taacttctgt aaataaagga cttgaaaagt tcccaggagt gcttttattt acttctcatg
    11341 accatgaatt tatatctact atagcaaata gaattataga gataactcca aatggaataa
    11401 tggatagaaa aatggacttt gatgagtatt tagaaagtaa agagatacaa gaccaattag
    11461 ctaaaatgta tggaaaagat aaataattat tagtataaaa ccttttaata tttgagacat
    11521 gctatgcatg agtgtgtaaa tttttaatca aatattagga ggttttttat gttgtttaaa
    11581 catgtttatt agcagaatag agtaatattt tgtatattaa gaatttaaat ataatatagt
    11641 agatgatata ataaaaatat ataacatttt ggggggaata gattatggtt aagaaaaggt
    11701 tagtagctgt agtattgagt ttagctatga ttagtgtggg gaatttttca tatgctcact
    11761 caggagggca taattcatca ggaggtcatc ataataaaaa taaatcagag ttaacacgtt
    11821 attattgtgg aggacattca gcacatcaac acaataaagg tttttgtcct tatgctagta
    11881 caaaagaaaa ttctggagta acaatatctt cagtacataa cgaaaaaaat cataaaaact
    11941 attttgcaga aaaagggtat aataaaggtt atgaagatgg gtgtaaaggg ctttataatt
    12001 taagtgtcac agaagccaaa tctgataatt tatatgaaaa agagatttta aaaagttcat
    12061 atgaaaaagg atatttaatt ggatacaatg aatataaagc aaaaaaagta gatgatgtaa
    12121 tgaagaagat gttagatatt ccaatgttag caagtatata ataatatagt atttaaaaag
    12181 atattttcta taagggaata tcttttttat tgtaaagtat aatttttcgt aaaaaagtat
    12241 aataactgag gcgataaatt aaaataaaac agattattta agtagttatt aatttgagat
    12301 agatacattt aatattaatg aatctatctc aataagctaa gctaatttaa agctaagata
    12361 aattaatatt aattagcatt aaataaactt tcagaaattt tgtcagaagc ctctttatcc
    12421 atttctttaa gtacatgaga ataaacgctt agagtagtac ttatatttga gtgtcctact
    12481 ctctcggata taacctttat aggtacttta gaatttataa gtaaagtaac atgtgagtgt
    12541 cttaaatcat ggaatctaat atgaggtaat tcattctttt ctaaaaatct tttaaatttt
    12601 ttactcataa catcttcagc aataggttca catttcttat tgaaaaacaa caaattatta
    12661 ttatttctaa tggaagattt taataaattt ttattctgtt ttattttata ttcttttaat
    12721 aaagacatta attctttggg agaagatatt tttctaacag agctttctgt ctttggctct
    12781 ttgagaataa cagaaccatt aattcgggaa gttatcttat ttactgttat tgtattttct
    12841 tcaaaatcaa tattatccca agttagacct aaaacttctg aaattctaag acctaatcca
    12901 atagccaaat taataggtaa ttccatttct gtattttttg ctaaatctaa taattttatc
    12961 atttgttctc tatcataaat ctcattttta aattttttat atcttggagc ctctatacta
    13021 tccataacat tttcttttat taatttgagt ctataagctc tttttattgc aagtttcaat
    13081 atatttatat gaatcttaat tgattgagga tttagtttat aagatagttt atctatgtag
    13141 ttttgtatgt gaaccggatg taagtcttgt attttatatt ttccaataga tggattaatg
    13201 taattcttac aaatactaac ataattatta tatgtagcta aagatatatt ttcactatat
    13261 ttatttagaa aatctaatag aaattctgac actgtaattt cgcttggtac taaaaaacca
    13321 tcattatata agctttcttt tacctcattt agtcttttac ttgcttccct ttttttatca
    13381 aaagtaccca tatttttttg ttttctttta cctgtctttt catcttcaaa ttccaggtaa
    13441 acaacatagt ttttatttct ttttctaata aatgcattca tatataaccc accaatcaaa
    13501 attttataca tttattataa catatatata tcacaaaata attttgaaat agagttgcat
    13561 acattttaat acattgtata tatttattta aaaaatgtat atattatgtc aaatattaga
    13621 attttaattt gataatctgt tatacttata gaatagatat ttatctttat attaatagat
    13681 tgtgtgttta attttgaata aaaatataaa ttttttgtat taataaattt ggatgagata
    13741 ttttaaataa aacctttgac tcgaaaagtc tattaatata gtatttaatt atgtagaagt
    13801 ttgagaggag tgtaaaatca tatgactatt actaagatac caccagcaga attaaaagtc
    13861 atgaaattta tttggagttc agattttaca gtagcatcaa aagatgtcat tgaagctatg
    13921 gaaaaactat ataattggaa gcaaactaca acactaacac ttttatcaag attagtaaat
    13981 aagcaattct taagtgcaag aaaaattgat agacatacac attatacagt tcttattgga
    14041 gaagaagagt atttacattt tgaaacaaaa acttttttag ataatatgca tggaagttct
    14101 attgagagtt ttatgaaatc cttgttcaaa aagggggtaa atgaagaaga attgaactct
    14161 gttgaaaaat gggttaaaga tattaaaaaa gatgaataat ttggtgtagt taattatcat
    14221 aaaataaata gtttttacaa taattagtta aatgaatcaa atataaagtt ttatgtaaaa
    14281 tttgtgattt ttataaatat ttttgtcttg ttatttaata ttaatacaat aagtttgatg
    14341 taaaatcaaa cctgtttgaa tttaatttat attgggtttg tcattaatat tattttagtt
    14401 tgatattgta tacgaattta aaagtcagaa tttttgtata
//
LOCUS       DBGJFF010000095        14392 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.096, whole genome shotgun sequence.
ACCESSION   DBGJFF010000095 DBGJFF010000000
VERSION     DBGJFF010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14392
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.096"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            631..4176
                     /locus_tag="ACOIAF_002771"
     CDS             631..4176
                     /locus_tag="ACOIAF_002771"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HGZ2669774.1"
                     /translation="MNRLKPRILGISILFIIIIILTCGAVFKFKFKLDNKLKETTHTY
                     LSEFTEHNIINLKTRLSGQFDMLESIANFIGNLNNINDEMVIDLMDSDVKRSSLIRMT
                     ISTLDGECYSNDGITHNVQDRDYFKKAKNGEKNISEPLKSIVNNKEIIVLSTPVYKSG
                     KIVGVLSGIYDSKKLNELLGLSAFDEKADVFVAKKNGDIVAQKTNKNRKTLSCIFQLF
                     DKDNCDEDDCLKTIKENMKNDKSGYFNYNLDNEKFMLNYQPLGVNDWYVFSVVPNKIV
                     SIQSENISYDVYIFALEILLVILIFIIYIACIINNNTKIIINEKEKFKVLTDNILGGV
                     KTCLKDEYMTMTYVSQGFLEFTGYSCEDLNKIFKNRFKDMIYKDDLDKFNCIILNQLE
                     KQDKIEVEYRIVRKDGSIIWILERSKSINDNKGSECIQGVLTDITELKKIQEELDSKR
                     FEIQAINDSIVGGIVITEINDDFDSIYLNEGYLNMIGYTRKQLEDELNNKLIKLIFKD
                     DRTELINSINKQLKLSDTFASEQRVEKRDGTIIWILLKGRKMIYDNGKSVLCCVIVDI
                     TSAKLLEQELKKSEERHEIILKQTQNIIIEWDIEKDKMNISPNWYNKFKYDPITENAT
                     KNIIKSVDIYHDDMDKFISLIKLTSQGSEYEECEIRLRTRDTIYAWFSIKATTLFDNN
                     GKPYKVVGSLTDIDESKREAEKLKERAEKDPLTDLYNKKASQSLIEEYITLDKKRKGA
                     LMIIDIDDFKGINDNLGHLYGDAVLSEIANDLINLFRATDIVGRIGGDEFIVFIKDIS
                     ETRDIINKAEELVKTFERSFLGKGRNYKISLSVGIAKFPQDGMDYMTLFRNADMALYS
                     AKGKGKNCYTLYDKKIDKVQYITNTRNAGDSDNVEMSFKEHITNYIFDILYESSDIEV
                     AVNLILSIVGKHFNVSRMYIFENSDDNRVCKNTFEWCNEGVTSEINNLKNICYKDLGN
                     YLLNFNADGLFYCNDIENLGSDVKKVLKPQGIRSILQCKILEGNEFRGFIGFDECSYN
                     RMWTNDEVNTLTFISKTLSMFLLKMRIQSKLEESLTIQKSVMDNMDIWAYVVDRNTYE
                     LLFINKKMLESAPNTHVGDLCYKAIWNNQDKPCKKCPMIYLKDGDDTYKSFMHNKKFE
                     VQANITTTTLKWFDGKDACLIFGSEITSIEAEENT"
     gene            4361..6295
                     /locus_tag="ACOIAF_002772"
     CDS             4361..6295
                     /locus_tag="ACOIAF_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HGZ2669775.1"
                     /translation="MENKFLPISKQDMIDRGWEELDFVLVTGDAYVDHHSFGTAIISR
                     VLESAGYKVGIIAQPDWRTTDDFMKLGKPRLAFLVNAGNMDSMVNHYSVSKKHREKDM
                     YSPGGKMGYRPDRATIVYCNKIREAYSDVAIVIGGIEASLRRFAHYDYWSDKVRKSML
                     IDSGADLLVYGMSEKQIVEVANALNDGYDPKYIRHIDGTCYISDTLEEIYDKYILIPS
                     YKEICEDKMKYVEAFKIQYDEQDPFRGNIIVQPHGSKYLVQNKPEKPLSREELDEVYG
                     LPYQKTYHPVYEKFGGIPAIEEVKFSIVSSRGCFGSCSFCAITFHQGRAVQSRSEKSI
                     IDEAIGITNLDDFKGYIHDVGGPTANFRRPACKKQITKGACKNRQCLSPSPCKNLDAD
                     HSEYLHLLRAVRKLPKIKKVFVRSGIRYDYVMADKNNKFLRELIEHHVSGQLKVAPEH
                     ISEEVLKYMQKPAGKTYDKFRQKFFAINEELGKKQYLIPYLMSSHPGSTLNSAIELAE
                     YLRDTHYQPEQVQDFYPTPGTLSTTMFYTGIDPLTMKPVYVPKSKRDKAMQRALLQYR
                     APRNYDLVYSALVEAGREDLIGFGHRCLIKPKNEKPYFNRNNSKKNVSKGTNKNKKTN
                     TNNRSNKNQQKSSTKKKKKH"
     gene            6401..7396
                     /locus_tag="ACOIAF_002773"
     CDS             6401..7396
                     /locus_tag="ACOIAF_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MqnA/MqnD/SBP family protein"
                     /protein_id="HGZ2669776.1"
                     /translation="MKKYISIILLVVLTMVVLVGCSPGKDNPKNKELSVVKQVKVAVP
                     DGLPAVAIAKLANENPEIKEGYETVYSIEKTPEAISTRVMKKDADIAIVPSNMAAIAY
                     NKTSSYNIVGTVGMGSLYLVSTENIKDYSDLVGKEVGCTGKGLTPDITIKSLLLQKDI
                     NYSDIKFNYVNSASELVPLLATGKVKTGIVPEPALSALMSKNPDIKIIKSLNDSWKEV
                     SGSKDGYPQSTLIVKSEFLRDNKDFVDSFVGQLSNSIDWANKNPEELGAYSEKIKIST
                     ESKIIGKSLERANLKYIPVKDMIKDYKNYYEKLANFDDKTLGGKVPDEAIYFVEK"
     gene            7368..8153
                     /locus_tag="ACOIAF_002774"
     CDS             7368..8153
                     /locus_tag="ACOIAF_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2669777.1"
                     /translation="MKQYTLLRNKKNKEQFIDKMEVTLSCVILLFLWQIIALKIDNDI
                     FLPTVGQVFNSIKEIMLNSSFYIDILYSMGRCFFSFLLAMIFAIILSIVSYLNRFFRN
                     LLKPINALTRSIPTMILVVLALIWFEKDSTPFIVGFTIVFPILYDNVLGAILNIDKNL
                     LEMANVYKIRFIDKVLKIYLPAIKFQIISILVSTFSLSLKVAIAGEVYSQPTYGIGTM
                     IQTEKINFNTSGIFAWIIIVLLISAILQIAQKFLARRAFLWKR"
     gene            8141..8839
                     /locus_tag="ACOIAF_002775"
     CDS             8141..8839
                     /locus_tag="ACOIAF_002775"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2669778.1"
                     /translation="MEKIKLSIENINKRYDSRIIFRDFNIDFYVNEVNCILGKSGCGK
                     TTLLNIISGIIKNDTNNLNIKENLNRVGNKLEASYIFQDDRLIDWLTVEENIKIVVNK
                     YYNKTQLNKICDEYLELVGISDYKKFYPQMLSGGIRQRVNIARAFIYPSKNIIMDEPF
                     KSIDAKNTQLIMDNFRNILRKEKRTVLFVTHNIEEALFLADRIFILGDSPIRIKKILK
                     NSKELEKNEVLKLI"
     gene            8962..9435
                     /gene="ybaK"
                     /locus_tag="ACOIAF_002776"
     CDS             8962..9435
                     /gene="ybaK"
                     /locus_tag="ACOIAF_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HGZ2669779.1"
                     /translation="MVKTNAMRILDSNKIDYKVMSYEVKSEHVDGVEVAHDIGRDVNE
                     VYKTLVTQGVSKNIYVYVIPVHENLDLKKAAKVAKEKSVEMIHVKDINKLTGYIRGGC
                     SPIGMKKFYKTFVNESAKNLDTIIVSAGKIGYQIELSPFDLQRLIKVEFVDVIKK"
     gene            9632..10471
                     /locus_tag="ACOIAF_002777"
     CDS             9632..10471
                     /locus_tag="ACOIAF_002777"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HGZ2669780.1"
                     /translation="MRKIKSEQIVEQVKKLCIGASLYLGEDVLSCIKEKAKSEKSEVG
                     KNILNILVENAEIAKEKNIPICQDTGMAVFFVEIGQEVLIEGDTLTDAINEGVRQGYE
                     EGYLRKSVVSPINRVNTKDNTPAVIHYDMVKGDKIKIEFAAKGFGSENMSKMKMLKPS
                     DGLEGIKKFIIDTVSEAGPNPCPPMVIGVGIGGTVDKCAQIAKKALFRELGEFNKDEN
                     IAKLESELLTAINKLGIGPQGLGGTTTALGLNIETFPTHIAGLPVVVNINCHASRHKK
                     VVI"
     gene            10484..11032
                     /locus_tag="ACOIAF_002778"
     CDS             10484..11032
                     /locus_tag="ACOIAF_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HGZ2669781.1"
                     /translation="MIKITTPVNEIDIAKLNCGDTISLSGILYTARDAAHKRLIDCIN
                     KGEELPFDVYGQGIYYVGPTPTKPGEVIGAAGPTTSYRMDDLTIPLLERGLRLMIGKG
                     KRSDEVIEGMQKYGAVYLAAIGGAGAYISNSIKSCEIIAYEDLGAEAIRKIVVEDLKL
                     TVAIDSYGNNIYEQGRAIYECK"
     gene            11098..12279
                     /locus_tag="ACOIAF_002779"
     CDS             11098..12279
                     /locus_tag="ACOIAF_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HGZ2669782.1"
                     /translation="MSKDYAKLALEMHEINKGKVSVESKVEIKTKDDLSTAYTPGVAE
                     PCLKIHENQDDVYRYTSKGNLVAVVSDGSAVLGLGNIGAEASIPVMEGKAILFKQFAD
                     VDAFPICLKTNDVDEIVKSVELMEPVFGGINLEDISSPRCFEIEERLKKSLSIPVFHD
                     DQHGTAIIVAAAIINSIKLIENKKIEDLEIVINGAGAAGIAIAKILLNMNVKNIILCD
                     RSGALEASIENLNYVQKEMLKVTNIRNEKGPLKDIIKGKDVFIGVSGPGAVTKEMVES
                     MSEKPIILAMANPTPEIMPEEAKLGGAFIMGTGRSDFPNQVNNVLAFPGIFRGALDVK
                     AKEINEEMKIAAAYAIANTISDEEICPEYILPDVFNKNVVKNVAKAVKEAAIKTKVNR
                     I"
     gene            12570..12956
                     /locus_tag="ACOIAF_002780"
     CDS             12570..12956
                     /locus_tag="ACOIAF_002780"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGZ2669783.1"
                     /translation="MEYKVNDQELNASMFIPFVNRIWPGDYDMDKTQTALSKTLNITA
                     YDDKVLVGCLRVLSDGYYFGTITELLVLPEYQKQGIGSKLFQLAKENTPTMLYFGAQP
                     GVEEFYEKNGCQRSLQSYIIKKKDSY"
     gene            13106..13369
                     /locus_tag="ACOIAF_002781"
     CDS             13106..13369
                     /locus_tag="ACOIAF_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HGZ2669784.1"
                     /translation="MEETKFCQCCAMPMGQTDELYGTNKDGSKSTDYCSYCFKDGEFT
                     ADISMEEMIEVCIPHMLQSNKDMTEDEARTMMNNFFPTLKRWK"
     gene            complement(13498..14340)
                     /locus_tag="ACOIAF_002782"
     CDS             complement(13498..14340)
                     /locus_tag="ACOIAF_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1-like domain-containing RNA-binding protein"
                     /protein_id="HGZ2669785.1"
                     /translation="MINIGDFNKLTVKRKTEFGYFLDGQTNNTKDDILLHNRLIGKNE
                     INIGDEVNAFIFKDSDDRTAATLIPPLAKVGDVAHLKVVDNTDIGTFIDMGLPKDILV
                     PFKAKTYPLFRDEKYLFYIYLDKSKRIAATTDIDSYLLTDHTYNVGDIVTGVVYGFQT
                     NNSAMICVDNKYAGVILHNEYFTELKTGDVLENLHVIKIYEDGKLGLSPRGNRKDELD
                     TLENKILSYLEGSDGYMRFNDKSDPKDISILFNSSKKNFKRALGVLMKKGLIYQDEEG
                     TYLK"
BASE COUNT         5740 a         1487 c         2532 g         4633 t
ORIGIN      
        1 atttgtatat ttataaggtt ttaaatatgt atttataata gtactgttat atatttttat
       61 tataattata aaaaagttaa taaaaaaagt gattttgaaa tttatatttt atttttataa
      121 atataattat tatatttata aaaataaaat agtaaaagga tattataata ttttactaga
      181 attattatct ggtaacttaa gttttgagta ttaatctaaa gtatatgttt ttatagggtt
      241 taaattttat gtatacaaaa ttaatttgct aactgaatca tataaaaaaa acaaaatatt
      301 ttttagaggg aatgattaga tactattttc tttgttgtga aattaattga agagtaatta
      361 attagaaaag gaatgaataa taatgaatat attacattat tactttttga taaatcaaaa
      421 ataaagttat agtttttaat attaattaag aatgatgaaa tatgaataaa gattttacaa
      481 ataaatctta agctatctta tttaattcta atacaggaag aaggtaaatg tatattgaaa
      541 aaatcataga aatagaagta aacaatttaa ataaatacaa aggtttgcaa aagaagtaat
      601 ataattatat acaatgagga tgtgagtatg atgaacagac taaagccaag aattttaggt
      661 attagtatct tgtttattat tataattatt ttaacatgtg gagctgtttt caagtttaaa
      721 tttaaattag ataataaatt aaaagaaacc acacatacat atttgtctga gtttacagaa
      781 cacaatataa ttaatctcaa gacaagactt agtggtcaat ttgacatgct tgaatctatt
      841 gctaacttca taggaaactt gaataatata aatgatgaaa tggtgattga tttaatggat
      901 tctgatgtaa agcgcagttc tttaattaga atgactatct ctacattaga tggggaatgt
      961 tattctaatg atggaattac ccataatgtt caagatagag attattttaa aaaagctaaa
     1021 aatggagaaa agaatatatc agagccttta aaatctattg ttaataataa agaaataata
     1081 gttttatcta ccccagttta taaaagtggg aaaatagttg gtgttttaag tggaatttat
     1141 gattcaaaaa agttaaatga gttattagga ttatctgcat ttgatgaaaa agcagatgtt
     1201 tttgttgcaa agaaaaatgg agatatagtt gctcaaaaaa caaataaaaa tagaaagacg
     1261 ctatcatgta tatttcagct ttttgacaaa gataattgtg atgaagatga ttgtctaaaa
     1321 actataaaag aaaatatgaa aaatgataag tctggatatt ttaattataa cttagataat
     1381 gaaaaattta tgttaaacta tcagccactt ggagttaatg attggtatgt tttttctgtt
     1441 gtgccaaata aaatagtatc tattcagtca gagaatatat cttatgatgt atatattttt
     1501 gcattagaaa tactactagt tatattgatt tttataatat atatagcatg tattataaat
     1561 aataacacca aaattattat aaatgaaaaa gaaaaattta aagtactcac tgataatata
     1621 ttaggagggg ttaaaacttg tttaaaagat gaatatatga ctatgacata tgtaagtcag
     1681 gggtttttag agtttacagg ttactcttgt gaagatttaa ataaaatatt taaaaataga
     1741 tttaaagaca tgatttataa agatgattta gacaagttta attgtattat tctaaatcaa
     1801 ttagaaaaac aagataaaat tgaagttgaa tatagaattg taagaaaaga tggaagtata
     1861 atttggattt tggagagaag taagtctata aatgataata aaggaagtga gtgtattcag
     1921 ggagttttaa ctgatataac agaacttaag aaaatacaag aagaattaga ttcaaaaaga
     1981 tttgaaatac aggctatcaa tgatagtatt gttggtggaa tagtgataac tgagataaat
     2041 gatgattttg atagtatata tttaaatgaa ggttatttaa atatgattgg atatacacgc
     2101 aaacaactgg aagatgagct aaataataaa ttaatcaaac ttatttttaa agatgataga
     2161 acagaattaa ttaatagtat aaacaaacaa ctcaaattaa gtgatacatt tgcatctgaa
     2221 caaagagttg aaaaaaggga tggtactatt atatggattt tattaaaagg aagaaagatg
     2281 atttatgata atggaaaatc tgtactttgt tgtgttattg tagatattac ttctgctaaa
     2341 ttattagagc aagaacttaa aaagagtgaa gaacgtcatg aaatcattct aaagcagacg
     2401 caaaatatta taatagaatg ggatatagaa aaagataaaa tgaatatatc acctaattgg
     2461 tataataagt ttaaatatga ccctataact gaaaacgcaa caaaaaatat aattaaatca
     2521 gtagatatat atcatgatga tatggataag tttatttcat taattaaact tacttcacaa
     2581 ggtagtgaat atgaagagtg tgaaattaga cttagaacta gagatactat ttacgcttgg
     2641 tttagtataa aggcaacaac tttatttgat aataatggta aaccttataa agtagtagga
     2701 agtttgactg atattgatga atcaaaacga gaagctgaaa agttaaaaga aagagcagaa
     2761 aaagaccctc ttacagattt gtataataaa aaagcatcac aatcacttat agaagaatac
     2821 ataacattgg ataaaaaaag aaaaggtgct ttaatgatta ttgatattga tgactttaaa
     2881 ggaataaatg ataatttagg acatttatat ggggatgctg tattaagtga aattgcaaat
     2941 gacctaataa atctatttag agcaaccgat atagtaggaa gaattggtgg agatgagttt
     3001 attgtattta taaaagatat ttctgaaaca agagatatta taaataaagc agaagaattg
     3061 gtaaaaacat ttgaaaggtc ttttttaggt aaaggacgaa attataaaat atcattaagt
     3121 gtaggaattg ctaagtttcc acaagatggt atggactata tgacactatt tagaaatgcg
     3181 gatatggctc tttactcagc caaagggaag ggtaagaatt gttatacatt gtatgataaa
     3241 aaaattgata aagtccaata tataactaat acaagaaatg caggagatag tgataatgta
     3301 gagatgtctt ttaaagaaca tatcacaaat tatatatttg atatactata tgaatcaagt
     3361 gatatagaag tggcagtaaa tttaattcta agtatcgtag gtaaacactt taatgtgagt
     3421 cgcatgtata tatttgaaaa ttcagatgat aatcgagtat gtaaaaatac atttgaatgg
     3481 tgtaacgaag gtgtaacctc ggaaattaat aatcttaaaa atatttgtta caaggattta
     3541 ggcaactatc ttttaaattt taatgcagat gggttatttt attgtaatga tattgaaaat
     3601 ttaggtagtg atgtgaaaaa agtacttaag ccacaaggca tacgttcaat attacaatgt
     3661 aaaattttag agggtaatga atttagaggg ttcataggat ttgatgaatg ttcatataat
     3721 cgtatgtgga ctaatgatga agtgaacact ttgacattta tatctaaaac gctcagtatg
     3781 tttttattaa aaatgcgtat tcagagtaaa ctagaagagt ctttaacaat tcaaaaatct
     3841 gtgatggata atatggatat atgggcatat gttgttgaca ggaataccta tgaattacta
     3901 tttatcaata aaaaaatgct tgagtcagca ccaaacactc atgtaggtga tttatgttat
     3961 aaagcgatat ggaataatca agataaacct tgtaaaaaat gccctatgat atatttaaaa
     4021 gatggtgatg atacttataa atcattcatg cataataaaa agtttgaggt acaggcaaat
     4081 ataactacaa ctactttgaa atggtttgat ggtaaagatg catgtttaat atttggaagt
     4141 gaaataacta gtattgaagc tgaagaaaat acttaaaata ctgtgttata tatatactta
     4201 aaatcgcaat ttgaatttat tagacttaaa atgtctatat aaatcattta agttgtggtt
     4261 ttaagtattt tttatgatat aattaagcgt taagaacaaa attaaaagat acttttgtat
     4321 aaaaaaatat ttgttaaata tatattgagc aggtgataat atggaaaata agtttttacc
     4381 aataagcaaa caagatatga tagatagagg atgggaagaa ttagattttg ttctagttac
     4441 aggagatgct tatgtggacc atcatagttt tggaacagca ataatatcta gagttttaga
     4501 aagtgctggg tataaagttg gaataatagc tcaaccagac tggagaacta cagatgattt
     4561 tatgaagcta ggaaaaccta gacttgcatt tctagttaat gcaggaaata tggattcaat
     4621 ggtcaatcat tattctgtaa gtaaaaagca tagagaaaag gatatgtatt ctccaggtgg
     4681 gaaaatgggt tatagaccag atagagctac tatagtatat tgcaataaaa taagagaagc
     4741 atacagtgat gttgcaatag taataggtgg aattgaagcg agcttaagaa gatttgcaca
     4801 ctatgattat tggagtgata aggttagaaa gtctatgtta atagatagtg gagcagattt
     4861 attagtttat ggtatgagtg aaaagcagat tgtagaagta gccaatgcac ttaatgatgg
     4921 atatgaccca aaatacatta gacatattga tgggacatgc tatatttcag atacattgga
     4981 agaaatatat gataaatata tattaattcc atcatataaa gaaatttgtg aagataaaat
     5041 gaagtatgta gaagctttta aaattcaata tgatgagcaa gacccattta gaggtaatat
     5101 aattgttcaa cctcatggaa gtaagtatct ggttcaaaat aagccagaaa aaccacttag
     5161 tagagaagaa ttagatgaag tatatggatt accatatcag aagacatacc atcctgtata
     5221 tgaaaaattt ggaggaatac cagctataga agaagtaaaa tttagtatag taagttcaag
     5281 aggctgtttt ggtagttgtt cattttgtgc aataacattt catcaaggta gagcagtgca
     5341 aagtagaagt gaaaaatcaa ttattgatga agcaatagga ataacaaatt tagatgattt
     5401 taaagggtat atacatgatg ttggaggtcc aacagctaat tttagaagac cagcatgtaa
     5461 aaaacaaatt actaaaggtg cttgtaaaaa tagacaatgc ttatctccat caccatgtaa
     5521 aaatttagat gcagaccaca gtgaatacct acatttactt agagctgtaa gaaaattacc
     5581 taaaataaaa aaggtgtttg tgcgttctgg aattagatac gattatgtaa tggcagataa
     5641 gaacaataaa tttcttagag agttgataga gcatcatgta agtggtcagt taaaggtagc
     5701 cccagaacat atatcagaag aagttttaaa gtatatgcaa aaaccagcag gaaaaactta
     5761 tgataaattt agacaaaagt tttttgctat aaatgaagaa ttaggtaaaa agcaatattt
     5821 gataccctat ttaatgtcta gtcaccctgg ttctacctta aatagcgcta tagagttagc
     5881 agagtattta agagatactc actaccaacc agagcaggtt caggattttt atccaactcc
     5941 agggactcta tctacaacca tgttttatac aggtattgac ccgcttacaa tgaagccagt
     6001 ttatgttcct aaatcaaaac gtgacaaggc aatgcaaagg gctttattac aatatagagc
     6061 acctagaaat tatgatttgg tatactctgc gttagttgaa gcaggaagag aagacttaat
     6121 tggttttgga catagatgtc ttataaagcc aaaaaatgaa aagccttatt ttaatagaaa
     6181 taattctaaa aaaaatgtat ctaaaggaac aaataaaaac aaaaaaacta atacaaataa
     6241 cagaagtaat aaaaatcaac aaaaaagttc aactaaaaaa aagaaaaaac attaaagtat
     6301 aaataaaaat acttaaaaaa gaaggattat tcgataaatt aacagaaatt agtataaata
     6361 aatataatta ttttaatgac gttacctttg ggaggaagag atgaaaaagt atatttctat
     6421 aatattatta gtggtgttga ctatggttgt tttggtagga tgttcaccag gaaaagataa
     6481 ccctaaaaat aaggagctat cagttgtcaa acaagtaaaa gtagctgttc cagatggact
     6541 accagcagtg gcaatagcta aacttgcaaa tgaaaatcca gagattaaag aaggatatga
     6601 aacagtgtat agcattgaaa agacacctga agcgatttcc actagagtga tgaaaaaaga
     6661 tgcagatata gctatagttc cttcaaacat ggctgcaata gcgtataata aaacttcatc
     6721 atataatatt gttggaactg taggaatggg ttcactttat ttggtgtcaa cagaaaatat
     6781 aaaagattat tctgatttag ttggtaaaga agttggatgt actggaaaag gacttactcc
     6841 agacataact ataaaaagtc ttttgttaca aaaggatata aattattcag atataaaatt
     6901 taattatgta aattcagcaa gtgaactggt accattattg gctactggta aagttaaaac
     6961 aggtattgtt ccagaacctg cacttagtgc tttgatgtct aaaaatccag acataaaaat
     7021 tataaaaagt ttaaatgact catggaagga agtaagtggt tcaaaagacg gttatcctca
     7081 atctacttta attgtaaagt ctgaattttt aagagataat aaagattttg ttgattcttt
     7141 tgtaggtcaa ttatcaaata gtatagattg ggcaaacaaa aatcctgaag aattaggtgc
     7201 atattctgaa aaaataaaaa tatctactga atcaaagata ataggaaaat ctcttgaaag
     7261 agctaattta aaatatattc cagttaagga catgattaaa gattataaaa attactatga
     7321 gaaattagct aattttgatg ataagacatt aggaggcaaa gtaccagatg aagcaatata
     7381 ctttgttgag aaataaaaaa aataaagaac aatttataga taaaatggag gtaacacttt
     7441 cttgtgttat cctcttattt ttatggcaaa ttattgcttt aaaaattgat aatgatattt
     7501 ttttaccaac agtaggtcaa gtatttaata gtataaaaga gattatgcta aattctagtt
     7561 tctatataga tatactttac tctatgggga gatgtttttt tagtttttta ctggctatga
     7621 tttttgctat tatactttca atagtgtctt atttaaatag attttttaga aacttattaa
     7681 aaccaatcaa tgctttgaca cgttcaatac caactatgat attggtagta cttgcactta
     7741 tttggtttga aaaggatagt acccctttta tagttggatt tactatagtt tttcctatat
     7801 tgtatgataa cgttttgggc gctatattga atatagacaa gaacttatta gaaatggcta
     7861 atgtgtataa aataaggttt atagataagg tgttaaaaat ttatttacca gccataaaat
     7921 ttcaaattat atctatacta gtatcaacat tctctttaag tttgaaagtt gctatagcag
     7981 gagaggtata tagtcaacct acatatggta ttggaactat gatacagact gaaaaaatta
     8041 actttaacac atctggaata tttgcatgga taattattgt tcttttaata tctgctattt
     8101 tacaaatagc tcaaaagttt ttagcaagga gagctttttt atggaaaaga taaagttgag
     8161 tattgaaaat ataaataaaa gatatgatag tcgtattata tttagagact ttaatattga
     8221 tttttatgta aatgaggtaa attgtatcct tggaaaatct ggttgtggaa aaactacact
     8281 cttgaacata ataagtggga ttattaaaaa tgacaccaac aatctaaata tcaaagaaaa
     8341 tttaaataga gtaggaaata agcttgaagc tagttacata tttcaagatg atagacttat
     8401 agactggtta acagtagaag aaaatataaa gatagttgta aataaatact ataataaaac
     8461 tcaattaaat aaaatttgtg atgaatactt ggaattggtt ggaattagtg attataaaaa
     8521 attctatcct caaatgctta gtggaggtat tagacaaaga gtaaatattg ctagagcttt
     8581 tatatatcca tcaaaaaaca ttataatgga tgaacctttt aaatctattg atgcaaagaa
     8641 tacacagctt ataatggata attttagaaa tatattaaga aaagaaaaga gaactgtgtt
     8701 atttgtaact cataatattg aggaagcatt gtttttagca gataggatat ttattttagg
     8761 agactctcca attagaataa aaaaaatatt aaaaaattct aaggaattgg aaaaaaatga
     8821 agttttgaag ttgatataga gttataataa tactgtcgtt ttaaaatata tttggttaaa
     8881 atctataaga ctatattaaa atagtacaga atattcaaaa ctgtgtataa tgttcgtatt
     8941 ttataaaaag gaagtgaatg tatggttaag actaatgcta tgagaatatt agattcaaat
     9001 aagattgatt ataaagtaat gtcttatgaa gtgaaaagtg aacatgtgga tggtgttgag
     9061 gtggcacatg atatagggag agatgtaaat gaagtgtata agacattagt aactcaagga
     9121 gttagtaaga atatatatgt atatgtaata ccagtacatg aaaacttaga cttaaaaaag
     9181 gctgcaaaag tagctaaaga gaaaagtgta gagatgattc atgtaaaaga tataaataag
     9241 cttacaggtt atattagagg cggatgttct cctataggta tgaaaaagtt ttataaaact
     9301 tttgtaaatg aaagtgcaaa gaatttagat acaattattg tgagtgcagg aaaaatagga
     9361 tatcaaatag aactatctcc atttgattta caaagactta taaaagtaga atttgtagat
     9421 gtaataaaaa aataagaatt acatataaaa tatagagctt atattctcaa taataggtgt
     9481 tgtaatatta ataaaatgaa taagaatttt aaaataaata ttcagctact tatgtgtaat
     9541 tggtataaaa atattgagaa attttaaaaa caatggtaaa gtgtaagtat aatcacgaat
     9601 attttttata aagtaatgga gggatgttcg cttgagaaaa ataaaatctg aacaaatcgt
     9661 cgagcaggta aaaaaacttt gtataggagc gagcctatat ttaggagaag atgttttaag
     9721 ttgtataaaa gaaaaagcga aaagcgaaaa gagtgaagtt ggaaaaaata tattgaatat
     9781 attagttgaa aatgctgaaa tagctaaaga aaaaaatata ccaatatgcc aagatactgg
     9841 tatggctgta ttttttgttg aaataggaca agaagtttta attgaaggag acacacttac
     9901 tgatgctata aatgagggag taagacaagg gtatgaagaa ggctacttaa gaaaatctgt
     9961 tgtaagtcca attaacagag taaatacaaa agataataca cctgctgtta ttcattatga
    10021 tatggtaaaa ggtgataaaa ttaaaataga atttgcagct aagggatttg gaagcgaaaa
    10081 tatgagtaag atgaaaatgt taaagccatc agatggattg gaaggaatta aaaaattcat
    10141 aatagataca gtttcagaag ctggacctaa cccatgccct cctatggtaa ttggagttgg
    10201 tataggtggg actgtggaca aatgtgctca aatagctaaa aaggcacttt ttagagaact
    10261 aggtgaattt aataaggacg aaaatatagc aaaacttgaa agtgaactat taacagctat
    10321 taataaactt ggtataggac ctcaaggctt aggtggtact acaacagctt tgggattaaa
    10381 catagagact tttcctacac acatagcagg tctacctgtt gttgtaaata taaactgcca
    10441 tgcatcaaga cataagaagg tagtaattta ggaggaaaat aaaatgataa aaataactac
    10501 acctgtaaat gaaattgata tagcaaaatt aaattgtgga gatacaatta gtttgagtgg
    10561 tatactttat acagcaagag atgctgctca taaaagattg atagattgta taaataaagg
    10621 tgaagaatta ccatttgatg tatatggtca aggaatatat tatgtaggac ctactcctac
    10681 aaagccagga gaagttatag gtgctgcagg tccaacgacg agttatagaa tggatgattt
    10741 gactatacct cttttagaaa gaggtcttag attgatgata ggcaagggaa aaagaagtga
    10801 tgaagttata gaaggtatgc aaaaatacgg agcagtttat cttgctgcaa taggaggagc
    10861 tggagcgtat atttcaaatt caataaaatc atgtgaaatt attgcatatg aagatttagg
    10921 agctgaagct ataagaaaga ttgtagtaga agaccttaag cttacagtag caatagatag
    10981 ctatggaaat aatatatatg agcaaggtcg agcaatttat gaatgtaaat aatgtttaga
    11041 aataaatatg aatatcaaaa ttaaaaatag ttgtgtatat tttaaggagg aaaattgatg
    11101 tctaaggatt atgcaaaatt ggcattagaa atgcatgaaa tcaacaaagg gaaagtaagt
    11161 gtagaaagca aggtagaaat taaaactaag gatgatttat caacagcata tacaccaggg
    11221 gttgctgaac cttgtttaaa aatacatgaa aatcaagatg atgtttatag atacacttct
    11281 aaagggaatc tagtagcagt agttagtgat ggaagtgctg tacttggatt aggtaacatt
    11341 ggtgctgaag catctatacc tgttatggaa ggtaaagcta tattatttaa acaatttgca
    11401 gatgtagacg cttttccaat atgtttaaag actaatgatg tagatgagat agttaaatct
    11461 gtagagttga tggagcctgt atttggaggt ataaatttgg aagacatatc ttctccaaga
    11521 tgttttgaga tagaagagag attaaaaaaa tctttaagta tacctgtttt tcatgatgac
    11581 caacatggga cagcaataat agttgcagca gccataataa actcaataaa gttaattgaa
    11641 aataaaaaaa tagaagattt agaaatagtt ataaacggag caggagctgc tggtatagcc
    11701 atagcaaaga tactacttaa tatgaatgtt aaaaatataa tcttatgtga tagaagtgga
    11761 gcattggaag ctagtataga aaatctaaac tatgttcaaa aagaaatgtt aaaagtaaca
    11821 aatataagaa atgaaaaagg acctttaaaa gatattataa aaggaaaaga tgtatttatt
    11881 ggtgtttcag gtccaggagc tgttacaaag gaaatggttg aaagtatgtc tgagaagcca
    11941 ataatattgg caatggcaaa tcctactcca gagataatgc cagaagaagc aaaattaggt
    12001 ggagcattta taatgggaac aggtcgttca gacttcccta atcaagtcaa taatgtactg
    12061 gcttttcctg gaatatttag aggtgcactt gatgttaagg ctaaagagat aaatgaagaa
    12121 atgaagatag ctgctgcata tgcaattgct aatacaataa gtgatgaaga aatatgccca
    12181 gagtatatat taccagatgt atttaataag aatgtagtaa aaaatgttgc taaggcagtt
    12241 aaagaagcag cgataaaaac taaagtaaat agaatataaa tttttatatg ttcatatatc
    12301 cttatattat cgggtatatg ggcattttta ttataggttt atactcatat atccttactt
    12361 ttatatacat atctttatga ttaaaggtgt tagaaaatgt agtaaatttt atactatcag
    12421 actctatctt tattacttta gtagatacat ggttgtaaag gtttatagaa aaaaatatag
    12481 gaaggcagat ttttattgtt gcgtatatat gagaatgata taatatgaat attataaatt
    12541 aataaattta aatttatgga ggtacaatga tggaatataa agttaatgac caagaactta
    12601 atgcttcaat gtttatccca ttcgtcaaca ggatatggcc aggagattat gatatggata
    12661 aaacacagac tgcattatca aaaactttga atataactgc ttatgatgat aaagtgcttg
    12721 taggatgtct gcgagttctt tcagatggtt attattttgg aacaattaca gaattgcttg
    12781 ttcttccaga gtatcagaaa caaggcattg gtagtaagct tttccagctt gctaaagaga
    12841 acaccccaac tatgctatat tttggtgcac agccaggagt agaagagttc tatgaaaaaa
    12901 atgggtgcca aagaagtctg caatcctaca taataaaaaa gaaagatagt tattaaagtt
    12961 ccagctttca agaatcttaa aagtacaata ttttagtgga atcatttcat tttttattac
    13021 taatatgaca taaacatgtc atattagcat tgatatactt ttaatatggt taagagccaa
    13081 agaaaaatat aaggagtgat ttaaaatgga agaaactaaa ttttgccaat gctgtgcaat
    13141 gccaatggga caaactgatg aattatatgg gacaaataaa gatggaagta aaagtactga
    13201 ttattgtagt tattgtttta aagatggtga gtttacagct gatataagta tggaagagat
    13261 gatagaagtt tgtattcctc atatgcttca aagtaataag gatatgactg aagatgaagc
    13321 aagaacaatg atgaacaatt tctttccaac attaaaacgc tggaaataat tttatgcaaa
    13381 attagttata tgcaaaacat gaaatatggt gttgagaata aggggtggtg tgctttatag
    13441 cataccacct gttttgttac ttggagttca gttattttcc taaatatttt aaatatacta
    13501 ttttagataa gttccctctt catcttgata aattaatcct tttttcataa gcactccaag
    13561 agctcgttta aaatttttct tacttgaatt aaataatata gaaatatctt tagggtcaga
    13621 tttatcatta aatctcatat atccatcaga accttctaaa taacttaaaa tcttattttc
    13681 taaagtatca agttcatcct ttctatttcc tctaggagaa agaccaagtt ttccatcttc
    13741 atatattttt atgacatgta aattttcaag aacatcacca gtttttagtt cagtaaagta
    13801 ctcattatga agtattacac cagcatattt attgtctaca catatcattg cagaattatt
    13861 agtttggaat ccatatacaa ctccagttac aatatctcct acattgtaag tatggtctgt
    13921 taataagtat gaatctatat ctgtagtagc agcaattctt ttgcttttat ctaaatatat
    13981 ataaaataaa tatttttcgt ctctaaatag tggataagtc ttagctttaa aaggaactaa
    14041 tatgtcttta ggaagtccca tatctataaa agtaccaatg tctgtattat caactacttt
    14101 taaatgtgct acatccccaa ccttagctaa aggcggtatt aaagttgcag cagttctgtc
    14161 atcagaatct ttaaatatga aagcatttac ttcatcacct atgtttattt cattttttcc
    14221 gattaatcta ttatgaagaa gtatatcgtc ttttgtgtta ttagtttgtc catccaaaaa
    14281 atatccaaat tcagtttttc tttttactgt aagtttatta aaatcaccta tgtttatcaa
    14341 attaaaatca tcctctcatt ttatatttat aatttagcca taatatactt at
//
LOCUS       DBGJFF010000096        14333 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.097, whole genome shotgun sequence.
ACCESSION   DBGJFF010000096 DBGJFF010000000
VERSION     DBGJFF010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14333
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.097"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            160..624
                     /gene="bcp"
                     /locus_tag="ACOIAF_002783"
     CDS             160..624
                     /gene="bcp"
                     /locus_tag="ACOIAF_002783"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HGZ2669786.1"
                     /translation="MLSIGTKAPEFTLEDKDGNKVSMSDFKGKKVVVYFYPKDNTPGC
                     TRQACAFRNAYDGFKKEDIQVIGISKDSIKSHQKFAEKHELPFILLSDPDLVAIKAFD
                     VWKEKKMYGKTALGVVRATYIIDENGIIEKVFEKAKPDTNAQEILEYLEKQE"
     gene            634..1377
                     /gene="yaaA"
                     /locus_tag="ACOIAF_002784"
     CDS             634..1377
                     /gene="yaaA"
                     /locus_tag="ACOIAF_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HGZ2669787.1"
                     /translation="MIIMLSPAKNMKNIEVFDRDLSLPCFIDNTKEIVENIKTFGIED
                     FKNKMKINEKLAVLNKNRFESIKFDRLGNPAILTYDGIQYKNIEAENFTRKDEEFANS
                     CIRIISGLYGVVKPYDSIYEYRLEMQTKLRVGEFKNLYEYWGNRIYKELIKEKTAIVN
                     LSSNEYSKSIEKFIKDSDTYITCTFKVNKNGILKVESTQAKKARGMMTKYIVKNRIRD
                     IEELKKFNLEGYKYKENLSNNSEYIFVKE"
     gene            1475..4162
                     /locus_tag="ACOIAF_002785"
     CDS             1475..4162
                     /locus_tag="ACOIAF_002785"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005388 - P-type calcium transporter
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HGZ2669788.1"
                     /translation="MKKRKEWSSKMENHKQMPWHSRATHEILESLDSSEEGLTDTEAE
                     KRLKENGLNELRKKPPRTTLQMLWSQITDAMVMILIGAAILSLIFGEFTEAFVILIIV
                     TVNAVIGIVQEKKAESSLEALKNMNSPTARVMRDGEENVIPASNLVVGDIVFLEDGAM
                     VPADLRLIETSSLKIQEASLTGESVPSEKDADTILPKECVLGDRSNMAYTSSIVTYGR
                     GVGVVVATGMDTEVGNIAHLLDSQDDFDTPIKRKLNTVGKTLSVIGIIVCVVIFAIGA
                     FYQRPLLPMFMIAISLAISIIPEGLPATATIVMALGVQRMAKRNALIRKLPAVETLGG
                     ATVICCDKTGTLTLNKMTVTHIAVNGDFESGKVTSIDTASEKHPLVYKEIVYAGALCN
                     DACINPDKTDEILGDPTEGALIFMAKKFGIDQEALEEEYPRVFEQPFDSDRKCMTTVH
                     NIDEKIIAYTKGAVDEILDLCTKILTSKGERNITEVDKKNIHELCLNMSKDALRVLGF
                     AKREISSIPKEDSENIEYDLTFIGMVGMIDPPRTEVIDAVSTCKEAGIRTIMITGDHK
                     VTATTIAHELGIWSEENTVISGDELDNLSDDELDEAVKNTTVFARVSPFDKLRIIQSL
                     KRIGEVPAMTGDGVNDSPALKSADIGIAMGISGTDVAKDSSDMILMDDSFTTIAYAIK
                     EGRRVYRNIQKVIQFLLVGNIAEILTLFVAILLNWDTPLLAVHILWVNLATATLPALA
                     LGVDPPSRNIMKHQPVKSTTLFEKNLIQRVVTQGIFVAIMTILAYKIGLNIENHSVGQ
                     TMAFCVLAFSQMLRAFSQRSNTDSMFNRNNGKNPFLLISFLTSAFLIAVILFAPVFRN
                     AFNLTVLNNLEWLITIVLAIMSVIQVEVGKIIKRKKLKK"
     regulatory      4216..4318
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            4428..5693
                     /locus_tag="ACOIAF_002786"
     CDS             4428..5693
                     /locus_tag="ACOIAF_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
                     /protein_id="HGZ2669789.1"
                     /translation="MYNKETICVQGNYKPGNGEPRVLPLYQSTTFKYSSIDQLAELFD
                     LKVDGHIYSRISNPTIQAFEEKISLLEGGVSSVAVSSGQSANMLAVLNICKSGDSILC
                     SSKVYGGTFNLLGPSLKKFGIDLISFDLDSSEDEIVELAKENTKVVFAETLANPTLEV
                     IDFEKIANVAKRINVPFIVDNSLASPVLCNPLKYGANIVTHSTTKYLDGHASSVGGII
                     VDGGNFNWDNGKFPELVEPDPTYHGISYTQKFGNAAYATKARVQLLRDYGNCLSPFNA
                     YLTNLNVETLHLRMERHSENALKIARFLEKHENVDWINYPGLEDNKYYENAKKYLSRG
                     CSGVLSFGVRGGLENAKKFVEKLQIASLVTHVSDVRTCVIHPASTTHRQLTEEQLIAS
                     GVLPSLIRLSVGIENVEDLIADLNQALNF"
     gene            5723..6613
                     /locus_tag="ACOIAF_002787"
     CDS             5723..6613
                     /locus_tag="ACOIAF_002787"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004414 - homoserine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HGZ2669790.1"
                     /translation="MALILPKGLPVINKLLDEGIDVIYKEDFKKELKYEENIDTKIAI
                     LNLMPIKIDTELDLLRRIDKTGFNVSVEFIKISTRESKRSCNEYVKEFYKTFDEAKGE
                     YFDGFIITGAPVEQMEFEEVDYWNELEEIMDYSKRKTKSTLYICWAAQASLYKYYNVK
                     KLPLSQKCFGVFKHKVDKDSKIVDGFENEFFAPHSRHTTVNIEALKENKELSIVSHSK
                     EAGPYIITNSRDVFVMGHSEYDKYTLDKEYKRDINKGDKISIPQNYYINDDPSEEPTV
                     KWKKHSELLFRNWIKNYLIQ"
     gene            7101..7544
                     /locus_tag="ACOIAF_002788"
     CDS             7101..7544
                     /locus_tag="ACOIAF_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HGZ2669791.1"
                     /translation="MDEMSFKKELLELTRDINMKFTTLLSDFYQPLGITAVQALILSE
                     LCEHGEKKISDLGKNLNMTNSNVSVICQRLEKNGFLNRIRDIEDQRIVKVKVTNKSLD
                     IQEHISSSIFDSYFENMTSEKLQDMEDIIEGLEKLNKLLTYIDCK"
     gene            7575..9392
                     /gene="ftsH"
                     /locus_tag="ACOIAF_002789"
     CDS             7575..9392
                     /gene="ftsH"
                     /locus_tag="ACOIAF_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HGZ2669792.1"
                     /translation="MDEHKPQKRSALYYYTVIIIVVMFLNALIFPSLMDKKMVEIDYG
                     KFLTMLDTGKVKSVEIENNRIAINPSSSKDKNIYITGRMDDPELVDRLKNAKVEFTKV
                     IPKENSPLLSILLTWIIPIGILMLFGNLMMKSMQKRMGGNAMQFGKSNAKMYVSAQSG
                     KRFNDVAGQDEAKEALTEIVDFLHNPEKYKKIGAQMPKGALLVGPPGTGKTLLAKAVA
                     GEANVPFFSISGSEFVEMFVGMGASRIRDLFKQAKEKAPCIVFIDEIDTIGKKRDNGS
                     GLGGNDEREQTLNQLLSEMDGFDGGIGVVILAATNRPDSLDKALLRPGRFDRRIPVEL
                     PDLGGREAILKVHAQNVNVSSDIDYNAIARSTSGASGAELANIVNEAALLAVKSGRDI
                     VEQQDFDESVETVIAGYQRKGAVISDKEKKIIAYHEIGHALVAAMQKHSAPVHKITII
                     PRTSGALGYTMQVAEDESVLMSKEEAIDKITTFTGGRAAEEVIFNTCTSGASNDIEQA
                     TKIARAMVTRLGMSEEFDMVALETVSNQYLGGDTSLACSSDTAAKIDEEVFKIVKACH
                     KNAIEILENNIDKLHELANYLLEKETITGDEFMEILNLN"
     gene            9570..12272
                     /locus_tag="ACOIAF_002790"
     CDS             9570..12272
                     /locus_tag="ACOIAF_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGZ2669793.1"
                     /translation="MDRPNPDILLEKIKNEEEKLSRGQLKIFFGYAAGVGKTYSMLES
                     AQNLKKVGVDVVVGYIEPHTRPETLALLDGLEILPVKEIEYKSIKLKEFDLDAALERK
                     PEVILVDEFAHSNVAGLRHTKRWQDIEELLLAGINVYTTVNVQHLESLNDIVEIITNV
                     SVKETIPDKFLDTNTQLELIDVEPDVLLERFNEGKIYKKEQATRAKNNFFIKDNLVAL
                     REIALRKTAERVNKEVQMTRLSKGDVTVIPTSDTLLACISPSPSSAKVIRTASRISDS
                     TFAKWIALYVETPNTARLPFDEQKQLQNNLKLAKKLGGEIIVLHGENIIEQILRIAKL
                     RNVTKIVIGRNHSSNKRFSKRFKKDIVDKLIDEVDYIDIHIIPYKFASDVKYKPKKDK
                     SSIKSKFKISKVDFIKLIFITLMISILAYAVQSMGFIRENILLIYMLGVVLVSMWTKG
                     YSTGIISSVFNIVLLNYFFTAPLYTLSIADSNYIVTLVVFSIVGIITSTLTSKIQHEA
                     ETAAKREENTKMIYQIIKGFLRLSNKDDIVNKGIELLSLSLSRDVICYLNVDKHNKSK
                     LYKKNTNNKDKNDLDSEDEKAVASWVLSNSTVAGNDTDTLPGSKGYYIPIVGMNLTLG
                     VVGISCIDSKLDTEDISLIETIIAQMAIALDREILSEAKENTNLEIERERLRSNLLRA
                     VSHDLRTPLAGISGAVSTIIKNKGTIGQDIIDELLSGVYEDTQWLIRLVENLLSMTKI
                     DEGKLEVKKHSELVEEIISEALQKIKKRIENALIDINIPEQILFVPMDAKLIEQVLIN
                     LIDNSLKYSKEDCKIEINVYEKDDYVWFEVSDNGPGISKELKKHIFDRFFTGEEGAKD
                     SRKGVGLGLSICKSIIQAHKGEIMVENNKDKGSTFKFSLPKENE"
     gene            12306..13004
                     /locus_tag="ACOIAF_002791"
     CDS             12306..13004
                     /locus_tag="ACOIAF_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGZ2669794.1"
                     /translation="MSKKLILLVEDDKTIRKFISTALLTQDYDVKEAITGKEGISIAV
                     SYSPDVVLLDLGLEDMDGIEVIKAIRQFSNIPIIVVSAREQDRDKVEVFDAGADDYLT
                     KPFSIVELLARVRVAFRHSQVEAQQKDDVKSTFEVDKLLIDFDKRKVIVDDVEVHLTP
                     IEYNILSLLAKYHGKVLTHNFIIKEIWGSVIGNETKSLRVFMATLRRKIEKQPANPRY
                     IITEVGVGYRLNDE"
     gene            13361..14317
                     /locus_tag="ACOIAF_002792"
     CDS             13361..14317
                     /locus_tag="ACOIAF_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HGZ2669795.1"
                     /translation="MYILNLNTRETIEDFRDKFYVAENSYLILSAPKNLKLLKETLDI
                     DEITFNDCLKFDEITKLDLFDNYDFLSLNTFELRDGEAVIEEVNMYLSDNFILVVVNE
                     EHFLFEFVKNIILKNSQLEKNPVINLFKINYLILREVIKNGFESLEKVEELILQIEDE
                     MMDNINKNHVSRISDVRGLTRIIVKNTRPLLYIGDRIVKENIRYLKYSNVKKYNLENF
                     QGIDFGIDKLYNFALSTRELADKLLDIYSSRVGEKTNNLITKLTLLTAISAPLTIITG
                     IYGMNFRYMPELNWIYGYPATLFFMLCIIFVGIIIFKIKKLL"
BASE COUNT         5657 a         1606 c         2569 g         4501 t
ORIGIN      
        1 aactattttc agtctatttt aacatataaa tacatgaaaa ttatatattt atgaaacttt
       61 atacataaaa aataaaatat tttatttata tatagattta tagggtaata atataagcat
      121 agtataaaat tattaataaa aacaaaagga gagatattta tgttgagtat aggaacaaaa
      181 gcaccagaat ttacactaga agataaagat ggaaataagg taagtatgtc agattttaaa
      241 ggcaaaaaag tagtagtata tttttatcct aaagataata caccaggatg tacacgtcaa
      301 gcttgtgctt ttagaaatgc atatgatgga tttaaaaagg aagatataca agttattggg
      361 ataagtaaag atagtataaa atcacatcaa aaatttgcag aaaagcatga acttcctttt
      421 attttacttt ctgaccctga cttagttgct ataaaagctt ttgatgtatg gaaagagaaa
      481 aaaatgtatg gaaaaactgc attgggagtt gtacgtgcga catatattat agatgaaaat
      541 ggaattattg aaaaagtctt tgaaaaagca aaaccagaca caaatgcaca agagattctt
      601 gaatatttgg aaaaacagga gtaataagaa tttatgatta taatgttatc accagctaaa
      661 aatatgaaga atatagaagt atttgataga gatttgagtt tgccttgctt tatagataac
      721 acaaaagaaa tagtggaaaa tataaagaca tttggtatag aagattttaa aaataaaatg
      781 aaaattaatg aaaaattagc tgtattaaat aaaaatagat ttgaaagcat taaatttgat
      841 agattaggaa accctgcaat cttaacttat gatggaatac aatataaaaa tatagaagca
      901 gaaaatttta caaggaaaga tgaagagttt gcaaatagct gtattcgaat cataagtgga
      961 ttgtatggtg ttgtaaaacc atatgatagt atatatgaat atagattaga aatgcagaca
     1021 aaattacgag taggtgagtt taagaattta tacgaatatt ggggaaatcg tatatataaa
     1081 gaactaatca aagaaaaaac agctatcgtt aatctttcat caaatgaata cagcaaatcc
     1141 atagaaaaat ttattaaaga tagtgataca tatattacat gtacttttaa agtaaataaa
     1201 aatggcatat taaaagtgga atctacacaa gcaaagaaag ctagaggtat gatgacaaaa
     1261 tacattgtaa aaaataggat aagagatatt gaagaactga aaaagtttaa tttagaaggt
     1321 tataagtata aagaaaactt atctaacaat agtgaataca tatttgtaaa agagtagaag
     1381 ttttgttaaa tattaaatgg cattgttaaa tttaggtaaa atatacttag tgtgtattga
     1441 attgaacttg attttaaaat ataattattc ttaaattaaa aaaagaaagg agtggtcttc
     1501 caaaatggaa aatcacaaac agatgccttg gcattcaaga gcaacgcatg aaattttaga
     1561 aagtctagat tcatcagagg aggggctcac cgataccgag gcggaaaaac ggcttaaaga
     1621 gaatggctta aatgaactta gaaaaaaacc acctagaaca actttacaaa tgctttggag
     1681 tcaaattaca gatgctatgg ttatgatact aataggagca gcaatattat ctttgatatt
     1741 tggcgaattt acagaagctt ttgtaatact aataattgta acagtaaatg cagttattgg
     1801 aattgttcaa gagaaaaaag cagaatcatc attagaagca ttaaaaaata tgaactcacc
     1861 tacagctcgt gttatgcgtg atggtgaaga aaatgttatt ccagcaagta atcttgtagt
     1921 aggagatatt gtttttttag aagatggagc tatggttcca gcagaccttc gtttaataga
     1981 aacatcaagt ttaaaaattc aagaggcttc tttaacagga gaatctgttc catcagaaaa
     2041 agatgcagat acaatcttac ctaaagaatg tgtattagga gaccgtagca atatggcgta
     2101 tacatcttca atagtcacat atggtagagg tgtaggtgtt gtagtagcaa ctggtatgga
     2161 tacagaagtg ggtaacatag cacatctttt agattcacaa gatgattttg atactcctat
     2221 aaaacgtaaa cttaatactg ttggaaagac attaagtgtc ataggtatta ttgtatgcgt
     2281 tgttatattt gcaataggtg cattttatca gagacctctc ttgccaatgt ttatgattgc
     2341 aatttccctt gccatatcaa ttattccaga gggattacct gcaacagcta ctattgtaat
     2401 ggctttaggc gtacagcgta tggctaaaag aaatgcttta attagaaaat tacctgctgt
     2461 tgaaacatta ggaggagcta cagtaatatg ttgtgataaa actggtacgt taaccttaaa
     2521 taaaatgact gttacacata ttgctgtcaa tggagatttt gaatctggaa aagtgacatc
     2581 aatagatact gcatctgaaa aacatccatt agtgtataaa gagattgttt atgctggtgc
     2641 actttgtaat gatgcatgta ttaatccaga taaaacagat gaaatattag gtgacccaac
     2701 agagggcgca ttaattttta tggccaaaaa atttggaata gatcaagaag ctcttgaaga
     2761 agaatatcca cgtgtatttg aacagccttt tgattcagat agaaaatgta tgacaactgt
     2821 tcataatata gatgaaaaga ttattgctta tactaaagga gcagtagacg aaattcttga
     2881 cctatgtact aaaatattaa cttcaaaagg tgaaagaaac attaccgaag tggataaaaa
     2941 gaatatacac gaattatgtc ttaacatgtc taaagatgca ttgcgtgtac ttggttttgc
     3001 taagcgtgag atttcatcta taccaaaaga agatagtgaa aatatagaat atgatttaac
     3061 atttataggt atggttggta tgattgaccc accaagaact gaggtaattg atgcagtaag
     3121 tacttgtaaa gaagcaggta taagaaccat aatgataaca ggtgaccata aagttactgc
     3181 cacaacaatc gctcatgagt taggaatatg gagtgaagaa aatactgtga tatctggtga
     3241 tgaacttgat aatttgagtg atgatgaact agatgaagct gttaaaaata ctacagtatt
     3301 tgctcgtgta tccccatttg ataaacttag aattatacaa tctctaaaaa gaattggaga
     3361 agtaccagca atgactgggg atggtgtaaa tgactctcct gccttaaagt ctgctgatat
     3421 tggtatagcc atgggtataa gtggtacaga tgttgcaaaa gattcttctg acatgatttt
     3481 aatggacgat agttttacaa caattgctta tgcaataaaa gaaggtagac gtgtatatag
     3541 aaacatacaa aaagttatac aatttttgct tgttggtaat atagcagaga tacttacgtt
     3601 gtttgtagca atattattaa attgggatac tccgcttctt gctgttcata ttctatgggt
     3661 aaaccttgca actgctacgc ttccagccct tgctcttgga gttgacccac caagcagaaa
     3721 tataatgaag caccagccag tgaaatcaac cactttgttt gaaaaaaatc ttatacaaag
     3781 agttgttacc caaggtatat ttgtggcaat aatgactatt ttagcatata aaattggtct
     3841 taatatagaa aatcattctg taggtcaaac aatggcattt tgtgtattag cattttcaca
     3901 gatgttacga gcatttagtc agcgttcaaa tacagattca atgtttaata gaaataatgg
     3961 taaaaatcca tttcttttaa tctcattttt aacttctgca tttttaatag cagtaatact
     4021 atttgcacct gtttttagaa atgcttttaa cttaacagtt ttaaataact tagagtggct
     4081 tataacgatt gttttagcca taatgtctgt aattcaagta gaggttggga aaattattaa
     4141 aagaaaaaaa ttaaaaaaat agttgacaaa attaggaaaa atatataaga tataaaacat
     4201 agaaaaatta aataaatctt atccagagag gtggagggac tggccctatg aaacccggca
     4261 accagtatat ttatatacat tggtgctaaa tcctgcggcg aaagctgaga gatgagtata
     4321 attaagctta tacagagcct gagttatata ctcaggcttt atttatttta actctcatct
     4381 ggtaaatagt ttaatttttt ttactaaaat ctaaggggga tacaagaatg tataataaag
     4441 aaacaatatg tgtgcaagga aattataaac caggtaatgg agaaccaaga gtactacctt
     4501 tatatcaaag tacaactttt aaatatagca gtatagacca acttgctgaa ttatttgatt
     4561 taaaagttga tggacatata tattcaagaa taagcaatcc tactattcaa gcttttgaag
     4621 aaaaaataag tttactagag ggtggagtat cttctgtagc tgtatcatca gggcaatctg
     4681 caaatatgtt ggcagtttta aatatatgta aatcaggaga tagtatactt tgttcttcaa
     4741 aagtatatgg aggaacattc aatttactag gacctagtct taaaaaattt ggtatagatt
     4801 taatatcgtt tgacttagat tcaagtgaag atgagatagt agaacttgca aaggaaaata
     4861 ctaaggttgt gtttgcagaa acacttgcaa atccaactct tgaagtcata gattttgaaa
     4921 aaatagcaaa tgtagctaag agaattaatg ttccatttat tgttgataat tcattagcat
     4981 ctccagtgct ttgtaaccct ttaaagtatg gagcaaatat agttactcat tctaccacaa
     5041 aatatttaga tgggcatgct tcaagtgttg gaggaattat agtggatggt ggaaacttta
     5101 actgggataa tggaaaattt ccagaattag ttgagccaga cccaacatat catggtataa
     5161 gctatactca aaaatttgga aatgccgcat atgcaactaa agcaagagtt cagttgctta
     5221 gagactatgg aaattgttta agcccattca atgcgtatct tactaattta aatgttgaaa
     5281 cactacatct tagaatggag agacatagtg aaaatgcact taaaatagct agatttttag
     5341 aaaaacatga aaatgtagat tggattaatt acccaggact tgaagataac aagtattatg
     5401 agaatgccaa aaagtattta tcaagaggat gtagtggtgt tttatcattt ggagtaagag
     5461 gtgggttaga aaatgccaaa aaatttgtgg aaaaattaca gatagcatct ttggttacac
     5521 atgtttcaga tgtaagaact tgtgttatac atccagcttc aactactcat agacaattaa
     5581 cagaggaaca attaattgca tctggagtat tgccttcact aataagatta tctgttggaa
     5641 tagaaaatgt agaggattta atagctgatt taaatcaagc tttaaatttc taatattcta
     5701 agagaaagag aagtgaaaag atatggcttt aatactacca aagggattac ctgttattaa
     5761 taaattatta gatgaaggaa tagatgtaat ttataaagaa gattttaaaa aggaattaaa
     5821 gtatgaagaa aacatagaca ctaagatagc tatactaaat ctaatgccta taaagataga
     5881 tacagaatta gatttactaa gaaggataga taaaacagga ttcaacgtgt ctgtggaatt
     5941 tattaaaatt tcaacaagag aaagtaaaag aagttgtaat gaatatgtta aagaatttta
     6001 taaaacattt gatgaagcca aaggagagta ttttgatggc tttataataa caggagctcc
     6061 agttgaacaa atggagtttg aagaagttga ttattggaat gaactagaag aaattatgga
     6121 ttattctaag agaaaaacta aatctacatt atatatatgt tgggcagctc aagcaagtct
     6181 ttacaaatac tacaatgtaa aaaaacttcc actaagtcaa aaatgttttg gagtatttaa
     6241 gcataaggta gataaagata gtaaaatagt agatggattt gaaaatgaat tttttgctcc
     6301 acattcaaga catactactg taaatattga agctttgaaa gagaataaag agttgagcat
     6361 tgtgagtcat tctaaagaag ctggtcccta tataattaca aattcaagag atgtgttcgt
     6421 aatgggtcat agcgagtatg acaaatacac tcttgataaa gaatataaga gggatataaa
     6481 taaaggagat aaaatatcta tacctcaaaa ttactatatt aatgatgacc cttcagaaga
     6541 gccaactgta aaatggaaaa aacattcaga attgttgttt agaaattgga taaaaaacta
     6601 tttgatacaa taaattttaa gagtatataa atgttaacta aaaaaatata gaaatttata
     6661 ggtataaagt taatcttaat gatactttat accttatttt tttaaaattt gaccttataa
     6721 tagtagtttt atattttaag aaaatcactt taggtgtatg tagatagagg tggctttaat
     6781 tttatatatg gtgcgataat gggagactaa acctttatag tatcaataaa tttgtatttt
     6841 aacttttaaa agagattaaa aagggagaaa tcagtgtaaa atatatatag attttttatt
     6901 aaattttatt aaaaatatcc tgtataaaat taaatatcaa taagataaca aaatttcaat
     6961 tgaagttaca atattgtaat tgacaataaa tacttaatac tataatattt aatacattaa
     7021 acattatagt gttaagtatt tattacagta tgcacttcag cctaaatttg aaataaagta
     7081 ttttcgtagg gagcgtttgt atggatgaga tgagttttaa gaaagagcta ctagagttga
     7141 ctagagatat aaatatgaaa tttacaactt tattaagtga tttttatcag ccacttggta
     7201 ttacagcagt gcaagctttg attttatctg agttatgtga acatggagag aaaaaaataa
     7261 gcgatttagg gaagaactta aacatgacaa atagtaatgt atcagtaatc tgtcaaaggc
     7321 ttgaaaaaaa tggcttttta aatcgcataa gagatataga agaccaacga attgtaaaag
     7381 taaaagttac aaataaatca ttggatatac aagaacatat ttcaagtagt atttttgata
     7441 gttattttga aaatatgacc agtgaaaaat tgcaagatat ggaagatata atagaaggtt
     7501 tagagaaatt aaataaactt ttaacttaca ttgattgcaa ataacataaa aataatttaa
     7561 aggagaataa aattatggat gaacataaac cccaaaaaag gtcagcactc tattattata
     7621 cagttataat aatagtagta atgtttttaa atgctttaat atttccaagt ttaatggata
     7681 aaaaaatggt tgagattgac tatggaaagt ttctcacaat gttggataca ggaaaggtta
     7741 aatctgttga gatagaaaat aatagaatag caataaatcc atctagtagc aaggataaga
     7801 atatatacat aactggtaga atggatgacc cagaattggt agatagacta aaaaatgcaa
     7861 aagtagaatt tactaaggtt attcctaagg aaaattcacc tcttttaagt attttactta
     7921 catggataat acctataggt attcttatgc tatttggtaa cttaatgatg aagtctatgc
     7981 aaaagcgaat gggtggtaat gctatgcaat ttggaaaaag caacgccaaa atgtatgtat
     8041 ctgctcaaag tggaaaaaga tttaatgatg tagctggtca agatgaggca aaagaagctt
     8101 taactgaaat agtagatttc ttacacaatc cagaaaagta taaaaagata ggagcacaaa
     8161 tgcctaaagg agcactttta gtaggacctc caggtacagg aaaaacactc cttgcaaaag
     8221 ctgtagcagg tgaagcaaat gtaccatttt tctcaatatc tggttctgag tttgttgaga
     8281 tgtttgtagg aatgggagct tctaggattc gtgatttatt taaacaagca aaagaaaaag
     8341 ctccatgtat agtgtttata gatgagattg atactattgg taaaaagcgt gataatggaa
     8401 gtggattagg tggaaatgat gaacgtgaac aaactttaaa tcagttgtta agtgagatgg
     8461 atggttttga tggtggaatt ggagtagtaa tacttgctgc tacaaataga cctgacagtc
     8521 ttgacaaagc attacttcgt ccaggaagat ttgatagacg tataccagtt gaacttccag
     8581 accttggagg acgtgaagct atattaaagg tacatgctca aaatgtaaat gtaagctcag
     8641 atatagatta taatgcaatt gcaaggtcta catcaggagc atcaggtgca gaacttgcaa
     8701 atattgtaaa cgaagcagca cttttagcag ttaagtctgg cagagatata gtagaacaac
     8761 aggattttga tgaatctgtt gaaactgtaa ttgctgggta tcaacgtaaa ggagctgtaa
     8821 tttcagataa ggaaaagaaa attatagcat atcatgaaat aggtcatgca cttgttgctg
     8881 ctatgcaaaa acattctgca cctgtccata agattactat aatacctaga acttcaggag
     8941 cattgggata tactatgcaa gttgctgaag atgagagtgt acttatgtct aaagaagaag
     9001 caattgataa aataactact tttacaggtg gtcgtgctgc tgaagaggtt atctttaaca
     9061 cttgtacatc aggagcatca aatgatatag aacaagctac taaaattgca agagctatgg
     9121 ttactcgcct tggtatgagt gaagagtttg atatggtcgc tcttgaaact gtaagcaatc
     9181 aatatttagg aggagatact tctcttgctt gttcttccga cactgctgct aaaatagatg
     9241 aagaggtatt taaaatagta aaggcttgtc acaaaaatgc aattgaaatt ttagaaaata
     9301 atattgataa attgcatgaa cttgctaatt atttattaga aaaagaaact attacaggtg
     9361 atgagtttat ggaaatactt aacttaaact aaaatgatta tacacaagat aaaaatgcct
     9421 attgttaggc atttttattt tacaactaaa ttttttaaat tcatatttaa atctttacat
     9481 aatcttaatc attaaaaata catcttaact acaattttat atcggaattg atattataat
     9541 agtaatgaat ggtgtaagga ggttttttaa tggatagacc taatcctgat attctattag
     9601 aaaaaattaa aaatgaagaa gaaaaactaa gtagaggtca attaaaaata ttctttggtt
     9661 atgcagctgg agtaggaaaa acctactcta tgttggaatc tgcacaaaat ttaaaaaaag
     9721 tgggtgtaga tgtagtagtt ggatatattg aaccacacac tagaccagaa acattggctt
     9781 tacttgatgg cttggaaatt cttcctgtaa aggagataga atataaatct ataaaactaa
     9841 aagaatttga tttagatgct gctttagaaa gaaaaccaga agttatatta gtagatgaat
     9901 ttgcccattc taatgttgct gggttaaggc atacaaaaag atggcaagat atagaagaat
     9961 tgctgttagc aggcataaat gtctacacta ctgtaaatgt acaacattta gaaagtctaa
    10021 atgacattgt tgagataatt acaaacgtat cagttaaaga gactataccg gataagtttt
    10081 tggatacaaa tacacagtta gaacttatag atgttgaacc tgatgtactt cttgaaaggt
    10141 tcaatgaagg taagatatat aagaaagaac aagctactag agcaaaaaat aattttttta
    10201 taaaagataa cctggttgca cttagagaaa tcgcacttag aaaaacagca gagagagtta
    10261 ataaagaagt tcaaatgacc agactatcaa agggtgatgt aactgtaata cctacatcag
    10321 atacacttct tgcatgtata agtccatctc catcttcagc aaaagttatt agaactgcat
    10381 ccagaatatc agacagtaca tttgcaaaat ggatagcttt atatgttgaa actccaaaca
    10441 cagcaagact tccatttgat gaacaaaaac aactacaaaa taacctaaaa ttagctaaga
    10501 aattaggtgg agaaataatt gttttacatg gtgaaaatat aatagagcaa atacttagaa
    10561 ttgcaaagct gagaaatgta acaaaaattg taataggtag aaatcattca agtaataaaa
    10621 gatttagtaa aagatttaaa aaagatatag ttgacaaatt aattgatgaa gttgattata
    10681 tagatataca tataattcca tataaatttg cctcagatgt taaatacaaa cctaaaaagg
    10741 ataaatcatc aataaaatct aaatttaaaa ttagcaaagt tgattttata aaacttatat
    10801 ttattacttt gatgataagt attttagcat atgcagtaca aagcatggga tttataagag
    10861 aaaatatatt gttaatatat atgttgggtg tagttttagt ttctatgtgg actaaagggt
    10921 atagtacagg tataataagt tctgtattta atatagttct tttaaattat ttttttacag
    10981 caccattata tacattatct atagcagatt caaactatat agtaacactg gttgtattct
    11041 caatagtagg tataataact agcactctta cgtctaagat acagcatgaa gcagaaactg
    11101 ctgctaaaag agaagaaaat acaaaaatga tatatcaaat aattaaggga tttttaagat
    11161 tatcaaataa agatgatata gtaaacaaag gtatagaact tttgtctctt agcttaagta
    11221 gagatgtaat ttgttattta aatgtagata agcataataa atcaaagctt tacaaaaaaa
    11281 atactaataa taaggataaa aatgatttgg atagtgaaga tgaaaaagct gttgcaagtt
    11341 gggttttatc caattcaaca gtggcaggaa atgacacgga tacacttcct ggttcaaagg
    11401 gatactatat accaattgta ggaatgaatt taactttagg agtagtggga atatcatgta
    11461 ttgattcaaa attggataca gaagatataa gcttaattga aacaataatt gctcaaatgg
    11521 caatagcgct tgatagagaa atcttgtcag aagctaaaga aaataccaat cttgagattg
    11581 aaagggaaag attaagaagt aatcttttaa gagcagtttc gcatgacctt agaactccac
    11641 tagcaggaat atctggtgca gtaagtacaa ttataaaaaa caagggtact ataggtcaag
    11701 atataataga tgaattactt agtggtgtat atgaagatac tcagtggtta ataagacttg
    11761 ttgaaaattt acttagtatg accaagatag atgaagggaa attggaagtt aaaaaacatt
    11821 ctgaactggt tgaggaaatt atatcagaag cgttacaaaa aataaaaaaa agaattgaaa
    11881 atgcacttat agatattaat ataccagaac agatattatt tgttcctatg gatgcaaaat
    11941 taatagaaca agttttaatt aatttaatag ataattcttt gaaatattca aaggaagatt
    12001 gtaagataga gataaatgta tatgagaaag atgattatgt ttggtttgaa gtatctgata
    12061 atggtcctgg tatatcaaag gaacttaaaa agcatatctt tgacagattt tttactggag
    12121 aagaaggagc taaagattcc agaaagggtg ttggacttgg attatcaatt tgtaaatcta
    12181 taattcaagc tcataaaggt gaaattatgg tagaaaacaa taaagataag ggttcaactt
    12241 ttaagtttag tttgcctaag gaaaatgaat agttttaaat tttaaaatta gttttggagg
    12301 aagaaatgag taaaaaactt atattgttag tagaggatga taagacaata agaaaattta
    12361 tatctactgc acttttaaca caggattatg atgtcaagga ggctataact ggaaaagaag
    12421 gtatatctat agctgtctca tattctccag atgttgttct tttagattta ggcttagaag
    12481 atatggatgg aatagaggta ataaaagcta tcagacaatt ttcaaatata ccaataattg
    12541 tagtttcagc tagagagcaa gatagagaca aggtagaagt atttgatgca ggggcagatg
    12601 attacttaac aaaaccattc tccatagtag agttattagc aagagtaaga gtagcattta
    12661 gacattcaca agtagaagct caacaaaaag atgatgttaa atctacgttt gaagtagata
    12721 aattattgat tgattttgat aaaagaaaag ttattgttga tgatgttgaa gtccatttga
    12781 cacctattga atataatatt ctttcattac ttgcaaagta tcatggaaaa gtgcttactc
    12841 ataattttat aataaaagaa atatggggta gtgttatagg gaatgagact aagtctttaa
    12901 gggtctttat ggcaacgtta agacgaaaaa tagaaaaaca acctgcaaac ccaagatata
    12961 taattacaga agttggtgta ggatacagat taaatgatga atagttgtaa tatattaatt
    13021 attagattat gtgcttatta atatatatta aaattatata gtttagttaa aataagaatg
    13081 aagtgtatgt gcttgttgtc agtttagcac atgcatttta ttttttagct tttttagtat
    13141 caataatttt ttagtatcaa taatatgaaa tatgttttaa aataagtata tagtgtaatc
    13201 tgtaataaaa actttggtta aataataaat gtatttaaca cttaccttat aataaattta
    13261 atataaatat taaataataa ttattaaaat agtgtaaaat aattacagta aggattataa
    13321 aaaattttta gaatgtaaat tgaaatttag gagtagatgt atgtatatac tgaatttaaa
    13381 tacaagagag actattgaag attttagaga taaattttat gtagcagaaa attcttatct
    13441 tatattaagt gctccaaaaa atttaaaatt gcttaaggaa actttggata tagatgaaat
    13501 aacttttaat gattgtctta aatttgatga aataaccaaa cttgatttat ttgataatta
    13561 cgatttttta agcctaaata catttgaact aagagatgga gaagctgtta tagaagaggt
    13621 aaatatgtat ttatcagata attttatatt ggttgtagtg aatgaagaac actttttatt
    13681 tgaatttgta aaaaatatta ttttgaagaa ctctcaactt gaaaaaaatc cagttattaa
    13741 tctatttaag ataaactact tgattcttag agaagttata aaaaatggtt ttgagtcttt
    13801 ggaaaaagtt gaagagttaa ttcttcaaat agaagatgaa atgatggaca acattaataa
    13861 gaatcatgta tctagaataa gtgatgtaag aggattaaca agaattatag ttaagaatac
    13921 tagacctctt ttgtatattg gggatagaat agttaaggaa aatataagat atttaaaata
    13981 ttcaaatgta aaaaaatata atttagaaaa ttttcaaggt atagattttg gaattgacaa
    14041 attatacaat ttcgcattgt caacacgaga gttagcagat aaattattag atatttattc
    14101 ttccagagta ggtgagaaaa caaataactt aattacaaaa ttaaccttgt taacagccat
    14161 atcagcacca cttacaatta taactggtat atatggaatg aactttagat atatgccaga
    14221 attaaactgg atttatggat atccagcaac tttatttttt atgctatgta taatttttgt
    14281 tggaattata atatttaaaa taaaaaaatt attgtaaatt acagatgatg ttt
//
LOCUS       DBGJFF010000097        14180 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.098, whole genome shotgun sequence.
ACCESSION   DBGJFF010000097 DBGJFF010000000
VERSION     DBGJFF010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14180
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.098"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            127..1278
                     /locus_tag="ACOIAF_002793"
     CDS             127..1278
                     /locus_tag="ACOIAF_002793"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004452985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGZ2669796.1"
                     /translation="MELRPRSAKLEEFDKVIELINYVFRISRNHKPTMMEEFPLLLSK
                     NNIENMIIISEDDKVVSDVNYLIQDVSIQGNRLKVAAIGGVCTHPDYEKRGYSSKILD
                     KVEEKMFYDGVDIVIISGTRSLYSRRNCSLVKSFYKYTIKPEDVKIAYEIVEFDETNF
                     EKDNDLDKMIELYNQNSTRFIRTRDEFQKLLHAATIAWGPIGYKKVFIKENNNIIGYL
                     IIRTIKKEASTVGEVAEIGLNSVNVENILKYVANKFGLESLNYKVHVKNLKDQLKCNG
                     TKSLDYQQGTMKIINFTKLCDSLRSYFSQYVDFELLKYMEFKQVENKYIIKYKEEELV
                     IENLDKLNKLFFEKNEEQYNEFKHLKNIYEFATKAFPVDFPWTANLNYQ"
     gene            1396..2034
                     /locus_tag="ACOIAF_002794"
     CDS             1396..2034
                     /locus_tag="ACOIAF_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423639.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HGZ2669797.1"
                     /translation="MDILDDIAECILNMGVDNIENLVKIAIDKNIDVEDIYEYGLNKG
                     MIGALDKFENKEYYLSEVIVCTDALNKGINLLKGTGKVKKKSKGVILMSVVEGDTHEI
                     GKNIVKVMVEATGYKVIDLGVNRKSEDIIEEAIKNNVDIIGLSSMMTTTMENMKSVID
                     ELNMIEIDKRPKVIIGGGPVSMEFAEEIGADGYSSNAPKAVKLINKLIGGEV"
     gene            2035..3057
                     /locus_tag="ACOIAF_002795"
     CDS             2035..3057
                     /locus_tag="ACOIAF_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HGZ2669798.1"
                     /translation="MFSDMTLLQYINSEDRGFFLPDMGTNGLFLINKKAYEVYDSVDL
                     QLELAKTMDKYFESDFIYSFCDGITFCETLGLDTLRPDYDFPSVLTHTITDRDKLRKL
                     DVPDPYTSGRMPLNIESLSVISKSIKKPLYESIQGPFTLAGQLAGATQLLRCIITDKK
                     FVEELLEFTTELVRRYAVAANKAGAKYISIAEPTSVTLSKDRFDEFIVKNLNKIYNEL
                     DCWKGMHICGDTRELLDNMLSCNIDAVSLDQILDYEEIAPMIPRDIVLIGNLDPIKLL
                     GRSKPDKIRRETLKLLKKMRGYDNFLCDFGCNCLNTTPVENLQAAMKAGRISYKELDR
                     IDIDEL"
     gene            3047..3667
                     /locus_tag="ACOIAF_002796"
     CDS             3047..3667
                     /locus_tag="ACOIAF_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669799.1"
                     /translation="MNYNSNKNKYLLKDINLDDIFIDKNIVLKFLGYGNRKAPDTISK
                     IVEREIRNINDILDIKIYISEVDINKTPKECKKAFAVLYTIGDKIDFKMNDYMENCNM
                     MAGIALDKIGIVCLDYINEKIKMYLKGKYDSLKISHEIYPGDKDFEVERQKDIYNYVK
                     NKYNSIEIEINDYHQLSPIKSVAMLILMGDEENLESRCSKCPRKCF"
     gene            complement(3791..4477)
                     /locus_tag="ACOIAF_002797"
     CDS             complement(3791..4477)
                     /locus_tag="ACOIAF_002797"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HGZ2669800.1"
                     /translation="MKTYKFIFFDADDTLFDFKKSESHAFKKLLSEFDLEFNFENYIE
                     SYRNISDKLWLDLEKNIITLNELKLLRFELFANKISLDVDSETLSKMYLNFLGECTFL
                     IPDAIDILQYLKKKYTIVIITNGISAVQKKRLENSKIKGYIDGMVVSEELKISKPNPE
                     IFKYALKKFNCHDKSSALIIGDSLTSDVLGGINSGIDTCWLNSNNSINSTNHIPTYEI
                     NTLIELKKLL"
     gene            complement(4491..5036)
                     /locus_tag="ACOIAF_002798"
     CDS             complement(4491..5036)
                     /locus_tag="ACOIAF_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGZ2669801.1"
                     /translation="MNDLNLGEKIAEVRKKQNLSIRDLAKLADVTPSLLSQIERGLAN
                     PSVNSLKSIASSLNVPLFTFFVSEVDKKNLIVRHDNRKKVILPGSKEVIYEILIPDSS
                     GNLEFAIMDLAPNTSSCVDRITHNGDEIAYVLEGEVKLFMDDDEFTLSKGDSVKVPLG
                     TKHKWQNDSNSESKVIFAVIL"
     gene            5264..6511
                     /locus_tag="ACOIAF_002799"
     CDS             5264..6511
                     /locus_tag="ACOIAF_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HGZ2669802.1"
                     /translation="MQITTFGAIFGLLIAIILIIKKFQAVYSLMLGAFIGGLVGGANI
                     TQTVDFMANGAMNISPSILRALASGVLAGSLIKTGAVDKISEQIVKIFGEKRALFSIA
                     ISTMVLAGVGVNLDVSIITVAPIGLYIGRKLNYSKLSILLAMLGGGKAGNIISPNPNT
                     IAVADNFSVNLSSVMMSNIIPAIIGVVITVILASILINKGNKVQSYEILEQREDLPSL
                     FKSLCGPIIAIFLLFLGNVSPIVIDPMIALPIGGIVTLIVTGNLNNSREYLAFGLSKM
                     QGVCILLLGTGTIAGIIQMSELQQSTIGALQFLNMPQFLLAPVSGILMSLATASSTAG
                     ATIASSTFHDAIINGGLSPISGASIVNAGSSVFEQLPHGSLFHTSAGSINMDIGERFK
                     LIPYEALIGIVMTIISTSIQLVL"
     gene            6598..8592
                     /locus_tag="ACOIAF_002800"
     CDS             6598..8592
                     /locus_tag="ACOIAF_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HGZ2669803.1"
                     /translation="MNKKKIVIIGIIYSFLVVFSLTNMYVNMEYNLNVFEYIKKSLPF
                     TEEEKKWLEKHKNLIYSSDQSSPPLRYKGKEDGQYKGIIVDLINSLSIQIGRDFYFKP
                     NNWWKESFVNSIDDSIKFFDLIPSKERANKFIFTDPIYTLSANILKDKKSQDINSYMD
                     LKGKTVVIPEGDYSINFLKQKIQDINILLTPDIKTGVNHLMSGKVDVVVGDEPVLRYY
                     INNYGLSNKYSVLSNPIYTKKAVLAVPKQYEELVSILNKGIFKLQKNGVYKDLKKKWY
                     STYNEVDDILYERGIVPSIYLFIGIILISIYVFYSYTYLLKIEIKRKTEQVIENKKTL
                     EATFNSITDIIMLVDENNNIVESNKVLYDFMGEMSYKIADLISMIKGVIENTFSENTN
                     KTSEIEIHNKILKINTFPVEYKKNNTEYIVVLIKDITNDKIVEAKLLRENKMISIGQL
                     ASGVAHEIRNPLGIIRNNCYLLKDNVTMEEVNDCVKSIESNVDRASNIITNLLNFARI
                     SDDNLEHINIKNFIENIVKLQYKMLQLKNVEIKIDCEHNLICYINGESLKHVFINLIS
                     NSIDAIHQDGKIIIYCYEKNHCLFIDFKDNGEGIKEDVLKDIFNPFYTTKPIGEGTGL
                     GLYITYNEIKKNNGDISVESKLGVGTCFHIKIPLNKEVTI"
     gene            8592..9914
                     /locus_tag="ACOIAF_002801"
     CDS             8592..9914
                     /locus_tag="ACOIAF_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HGZ2669804.1"
                     /translation="MKILLVDDELEYGVVMKKILQKKGYLVDIALSGEEAINIIKKDK
                     NYDLVLSDVMMKNMDGVQLLDRIKSINRDIEVILVTGYGSIENAVDAMKRGALSYFIK
                     SNPIENLLEEVEKVKASKISVSVQKNNFEFTLESKNRDFNNIIKIAKKAACKDVNILI
                     LGESGVGKDILARYIHSISPRKNEIFVPVNCCSFSENLLESELFGHEKGSFTGAVDSR
                     KGRFELSNKGTLFLDEIGDIPLNVQVKLLRTLEDKSIERIGSNKSIKVDFRLICAMNK
                     EPKVEISNGNIREDFFYRISTITITIPPLRKRREDLTTLIEFFLNKYQIEHDKKIHSI
                     DKEVKDFLLNYNYPGNIRELKNIINRLVVLSEEGNLSKDNLNLISNNVYIDDKISIRP
                     LREIRKEFECEYIEKVLSLCGNNISNTAKKLEISRRQLTNKISEYNIK"
     gene            10193..11479
                     /gene="grdG"
                     /locus_tag="ACOIAF_002802"
     CDS             10193..11479
                     /gene="grdG"
                     /locus_tag="ACOIAF_002802"
                     /EC_number="1.21.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006807454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine reductase complex component B subunit
                     alpha"
                     /protein_id="HGZ2669805.1"
                     /translation="MKLELGNIFIKNVEFGEKTEVKDGVLYISSEEIEKIVLEDERIV
                     SVNVELARPGESIRIAPVKDVIEPRVKIGDESKIFPGIINKVKTVGSGRTHVLLGACV
                     VTCGNIVGFQEGVIDMSGPTAKYTPFSKTNNICIVIKAKDGIDTHDYEEAARIAGLKI
                     GAYVGEAGREVEPDEVIVYETKPLLKQVKEYPDLPTVAYVHMLQSQGLLHDTYYYGVD
                     AKQIVPTFMYPTEIMDGAIISGNCVAPCDKVTTFHHLNNPVIHDLYKRHGKDLNFIGV
                     ILTNENVFLVDKERSSDMVAKLIEFLGVDGVLVTEEGYGNPDTDLMMNCRKCSEVGAN
                     VVLITDEFPGKDGKSQSIADATKEADAVVSCGQGNLVVHFPAMEKIIGTLDYVEMMIG
                     GYKGCLNEDGSMDAELQIIIASTIANGYNHLTARYY"
     gene            11565..12872
                     /gene="grdF"
                     /locus_tag="ACOIAF_002803"
     CDS             11565..12872
                     /gene="grdF"
                     /locus_tag="ACOIAF_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_082007859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:12612..12614,aa:Sec)
                     /transl_table=11
                     /product="sarcosine reductase complex component B subunit
                     beta"
                     /protein_id="HGZ2669806.1"
                     /translation="MSKIRVVHYINNFFAGIGGEEKADIPPEKRAGVVGPGIAFQSQF
                     NDDAEIISTVICGDTYFGENIESATQELLKMIKEESPDLFIAGPAFNAGRYGVACGTI
                     CKAVEEELNIPVITGMYKENPGVDMFKLDLHIVSTGNSAASLRKVVPIMTKLGLKLVR
                     GEEIGPPEEEGYIMRGIRKNFFHELRGSERAIDMLVKKMKGESFETEYPMPEFDRVTP
                     MKPVKDLSKIKLALVTSGGIVPIDNPDKIESSSATKYGIYDLTDMDSMSNKEFTTIHG
                     GYDRAYVLENPNLVVPLDVVRELEKEGVIGELVDYFITTTGTGTSVGNSKRFGEEFSK
                     KLLEDDVDAVILTSTUGTCTRCGATMVKEIERAGIAVVHICTVVPISLTIGANRIVPA
                     VGIPYPLGNPMLGEEESKKIRKNIVLKALNALTEDIEEQTVFE"
     gene            13165..13881
                     /locus_tag="ACOIAF_002804"
     CDS             13165..13881
                     /locus_tag="ACOIAF_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HGZ2669807.1"
                     /translation="MYILISIAICDDESYMLNHLKENIELYMKQKNLLYNIELFNKVE
                     NLLCNHEKFNIVFLDIQMEEINGMQAARKLRSYGNECFIIFVTVLNEFVFDAFEVDAI
                     NYLIKPLSNEKLYKTLDKIVTKLDDNESNYITIQKGYSYWKLKLADILYCEVIKRKIY
                     VHLKKETVDYYETIENLERSLPTNFFRCHRSYIINLQYVYGYENGNAVMESGDRIPVS
                     RLRQQIFSKVMLEFMKNRRI"
     gene            complement(13975..>14180)
                     /locus_tag="ACOIAF_002805"
     CDS             complement(13975..>14180)
                     /locus_tag="ACOIAF_002805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669808.1"
                     /translation="VDTIDKTVDCFIMYFTNKKVIRKNRICEENKLYKLKYVFIMIKK
                     IEIIEYKIKHQNFELKFVKLIRK"
BASE COUNT         5672 a         1416 c         2447 g         4645 t
ORIGIN      
        1 aaaatttgct aaaaaaaaca attaaaaatt gtaaagaaaa aagatataat tagaactgta
       61 gtaataaatg ttttattata gataatagag ttttaagtat cttaaataat atttggagga
      121 atatgaatgg aattaagacc aagaagtgca aaattagaag aatttgataa agtaatagaa
      181 ttaataaact atgtatttag aatatcaaga aatcataaac caactatgat ggaagaattt
      241 cctcttttac taagtaaaaa taatatagaa aatatgatta taatatcaga agatgataaa
      301 gtggtttctg atgtaaacta tctaattcag gatgtatcta ttcaaggaaa cagattgaag
      361 gttgcagcta taggtggagt atgtactcat ccagattatg aaaagagagg ctattcatct
      421 aagatattgg ataaagtaga agaaaaaatg ttttatgatg gtgtagatat tgtaattata
      481 tcaggaacaa gaagtctata cagtagaaga aattgtagtt tggtaaagag tttttataaa
      541 tatacaataa aacctgaaga tgtaaaaata gcatatgaaa tagttgaatt tgatgaaact
      601 aattttgaaa aagataatga tttagataaa atgattgaac tatataatca aaatagtact
      661 agatttataa gaacgagaga tgagtttcaa aaactattgc atgcagcaac tattgcctgg
      721 ggaccaattg gttacaagaa ggtatttata aaggaaaata ataatataat aggatatttg
      781 ataataagaa caataaaaaa agaagcttct actgtaggag aagttgctga aatagggtta
      841 aactctgtaa atgttgaaaa tatattgaaa tatgttgcaa ataaatttgg attagaatct
      901 ttaaattata aagtacatgt gaaaaatctt aaagaccagt tgaaatgtaa tgggacaaaa
      961 tctttggatt atcaacaagg aactatgaaa ataataaatt ttactaagtt gtgtgatagt
     1021 ttaagaagtt attttagtca atatgtagat tttgaattac taaaatacat ggaatttaaa
     1081 caagttgaaa ataaatatat tattaaatac aaagaagaag aacttgtaat agaaaatttg
     1141 gataagctta ataaactgtt ttttgaaaaa aatgaggaac agtacaatga atttaaacat
     1201 ctaaaaaata tatatgaatt tgctacaaag gcatttcctg tagattttcc atggactgca
     1261 aatttaaatt atcaatagtt ttagatgata ttaaataaaa ttgattgata cctagagtta
     1321 gtccttgtta aataagagaa attattggat attgaaaaag caattttgta agtaattaga
     1381 aaggatgagt attatatgga catacttgat gatatagcag aatgtatatt aaatatgggt
     1441 gtagataata tagagaattt agtaaaaatt gctatagata aaaatattga tgtggaagat
     1501 atttatgaat atggtttaaa caaaggtatg ataggagctt tagataagtt tgagaataag
     1561 gaatactatc tttcagaagt tatagtatgc acagatgcat taaataaagg tattaatctt
     1621 ttgaagggaa ctggaaaagt aaagaaaaaa tcaaagggag taattctgat gtctgtagta
     1681 gaaggagata ctcatgagat aggaaaaaat attgtaaaag taatggtaga agccactgga
     1741 tataaggtta ttgacttagg tgtaaataga aaaagtgaag atataataga agaagctata
     1801 aaaaacaatg tagatataat aggcttatct tcaatgatga caactacaat ggaaaatatg
     1861 aaatcggtta tagacgaact taatatgatt gagatagata aaagaccaaa agtaataatt
     1921 ggtggaggac ctgtaagtat ggagtttgca gaagaaatag gagcagatgg atacagttca
     1981 aatgctccaa aagcagttaa attaataaat aaacttatag gaggtgaagt ttaaatgttt
     2041 tcagatatga ctttacttca gtatataaat agtgaagata gaggtttttt tctgcctgat
     2101 atgggtacaa atggattgtt cttaataaat aaaaaggcat atgaagtata tgatagtgta
     2161 gatttgcaat tagaattagc aaagactatg gacaagtatt ttgaatcaga ttttatatac
     2221 tcattctgtg atgggattac attttgtgaa actctgggtt tagatacatt aagaccagat
     2281 tatgattttc caagtgtatt gacacatact ataactgaca gagataaatt aagaaaatta
     2341 gatgttccag acccttatac gagtggacgt atgccactca atatagagag cttatcagtt
     2401 atatcaaaaa gtattaaaaa accactatat gaatcgatac aaggtccatt tacacttgca
     2461 ggtcaattag ctggagctac tcaactttta agatgtataa taactgataa aaaatttgta
     2521 gaagaattat tggaatttac aactgaattg gtaagaagat atgctgtagc tgcaaataaa
     2581 gcaggagcaa aatatatttc aatagcagag ccaacatcag taaccctaag taaagataga
     2641 tttgatgaat ttatagtaaa aaatttaaat aaaatctaca atgaacttga ttgttggaaa
     2701 ggtatgcata tatgtggaga caccagagaa ttattagata atatgctaag ttgtaatata
     2761 gatgcagtaa gtttagacca aattttagac tatgaagaaa ttgctccaat gattcctaga
     2821 gatatagttt tgattggtaa tttagaccca ataaaacttc ttggacgttc taagccagat
     2881 aagattagaa gagagacact aaaattgtta aagaagatga gaggatatga taacttttta
     2941 tgtgattttg gctgtaactg cttaaatacc actccagtag aaaatttaca agcagctatg
     3001 aaggcaggaa gaattagtta taaagaatta gataggattg atattgatga attataatag
     3061 taataaaaat aaatatcttt taaaggatat aaatttagat gatattttta tagataagaa
     3121 tattgtgcta aagtttttgg ggtatggaaa tagaaaagct ccagatacaa ttagtaaaat
     3181 cgttgaaaga gaaataagaa atataaatga tattttagat atcaagatat atataagtga
     3241 agttgatatt aataaaacac ctaaagaatg taaaaaagca tttgctgtac tatatacaat
     3301 aggggataaa attgatttta agatgaatga ctatatggaa aattgcaata tgatggcagg
     3361 gatagcattg gataaaatag gtatagtatg tttagattat attaatgaga aaataaaaat
     3421 gtatttaaaa ggaaagtatg atagtctcaa aatatcgcat gaaatatatc caggagataa
     3481 agattttgaa gtagagagac aaaaggacat ttataactat gtaaaaaata aatataatag
     3541 tatagaaata gaaatcaatg attatcatca actatccccc ataaaaagtg ttgcaatgct
     3601 tattttaatg ggggatgaag aaaatttaga atcaagatgt agcaaatgtc ctagaaaatg
     3661 cttttaacta gttttcaaga ctaagatttg ttttgtgtat gaattgcaat aatttttttg
     3721 gtcattttat gtataaacta aaaaaattat tgcaaagtaa tattctattt aatttgaaca
     3781 gacttacaaa ctatagtagt ttctttaatt caataagtgt atttatttca taagttggta
     3841 tatgattagt agaatttata ctgttattgg aatttaacca acaagtatct attcctgaat
     3901 ttattccacc taagacatca gaggttaagc tatccccaat tattaaggca gaagatttat
     3961 catgacaatt aaactttttg agagcgtatt taaatatttc aggatttggt tttgatattt
     4021 tcaactcttc agatacaacc attccatcaa tatatccttt tatttttgaa ttttcaagtc
     4081 ttttcttttg aactgcagaa attccatttg ttattataac aatagtatac ttttttttaa
     4141 gatattgtaa tatatcgatt gcatctggaa ttaaaaatgt acattcacct aaaaaattta
     4201 agtacatttt gctaagagtt tcagaatcaa catccaaaga tattttattt gcaaaaagtt
     4261 caaatctaag taatttaagt tcattcaaag ttataatatt tttttcaagg tcgagccaaa
     4321 gcttatcact tatatttcta tatgactcta tataattctc aaaattaaat tctaaatcaa
     4381 actcagatag aagtttttta aaagcatgtg attcactttt cttaaaatca aataaagtat
     4441 catctgcatc aaaaaatata aatttatatg tcttcatggt ttttactcct ttacaagatg
     4501 actgcaaaaa ttactttact ttcactattt gagtcatttt gccatttatg ttttgttcct
     4561 aaaggaactt ttacactatc tcctttggac aaagtaaatt catcatcatc cataaaaagt
     4621 ttaacttctc cctccaaaac atatgcaatt tcatctccat tatgagtaat cctatcaaca
     4681 caagaagaag tgtttggagc taaatccata attgcaaatt ctaaattacc actagagtct
     4741 ggaattaaaa tctcatatat aacttctttg cttccaggta aaataacttt ttttctatta
     4801 tcatgtctta ctatcaagtt ttttttatct acttcagata caaaaaatgt aaataaagga
     4861 acgtttagag aagaggctat agattttaat gaatttacag atggattagc aagacctcgt
     4921 tctatttggc tcaataatga tggtgtaaca tcagctagtt ttgctaaatc tcgaatactt
     4981 aagttttgtt tttttcgaac ttcagctatt ttttccccta aatttaaatc attcatataa
     5041 atatcctctc tttttaattt aaaaaatatt aacttaaaat aatcaagtaa aatgattatg
     5101 tagtatgaaa ttattataac atatagaatt aggttttgaa aaaactatgt tcaatataat
     5161 taaataaatt aaaatataat tgacaaagtg gatacagaat acttaaaata tatttaaata
     5221 aaattaagca aaagatagtt aatttatgat aggaggggat attatgcaaa taacgacttt
     5281 tggagctata tttggtttat taatagcaat tattttaatt ataaaaaaat ttcaagctgt
     5341 atatagttta atgttaggag catttattgg aggattagtt ggaggagcaa atataacaca
     5401 aactgtagat tttatggcaa atggagctat gaatatatca ccatcaattc ttagagcttt
     5461 ggcatcaggt gtattagctg gaagtttaat aaaaacaggg gcagtagata aaatatcaga
     5521 acagattgta aaaatatttg gtgaaaagag ggctttattt tcgattgcca tttcaactat
     5581 ggtacttgct ggagtaggtg taaatttgga tgtatctata ataactgtag cacctatagg
     5641 attatatata ggtagaaaat taaattattc taaattgtca atactattag ctatgttggg
     5701 agggggtaaa gcaggtaata taatatcacc aaatccaaat actatagcag tagcagataa
     5761 tttttctgtc aatctctcta gtgttatgat gtcaaatata attccagcaa ttattggagt
     5821 tgttattaca gttattttag caagtatatt aataaataaa ggaaacaaag tacaatcata
     5881 tgaaatttta gagcaaagag aagatttacc aagtttattt aaatcattat gtggtccaat
     5941 tatagctata tttctattgt ttttaggaaa tgtatctcct atagttatag acccaatgat
     6001 agcccttcca ataggaggaa tcgtaactct aattgtcaca ggaaacttga acaattcaag
     6061 agaatattta gcttttggat tatctaagat gcagggggta tgcatattat tacttggaac
     6121 agggactatc gcaggaatta tacagatgtc tgaattacaa cagagtacaa taggtgcttt
     6181 acaattttta aatatgccac aatttttatt ggcaccagtt tcgggtatac ttatgtcatt
     6241 agcaacagca tcatcaacag ctggtgctac tatagcatca tctacttttc atgatgctat
     6301 aataaatggt gggctatcac cgatttcagg tgcatcaata gtaaatgctg ggtctagtgt
     6361 attcgagcaa ttaccacatg gctcactatt tcatactagt gcaggtagta taaacatgga
     6421 tataggtgaa agatttaagt taattccata tgaagcactg ataggaatag taatgactat
     6481 aatttcaaca tctattcaat tagttttgta ataaatataa atatttaatg aaaaaagtag
     6541 gattttgtta ttaaatattg aatttattat agtaaaaaag aagattggag ttttaaagtg
     6601 aataaaaaaa agattgttat tatagggatt atttattcat ttttagtagt attttcactt
     6661 acaaatatgt atgtaaatat ggagtataat ctaaatgtat ttgaatatat taagaagtca
     6721 cttccattta cagaagaaga aaaaaagtgg ttagaaaaac acaagaattt gatttacagc
     6781 tctgaccaaa gttcacctcc tttgagatat aaaggtaagg aagatggaca gtataaggga
     6841 attattgttg atttaataaa ttctctgtca attcaaattg gtagagattt ttattttaaa
     6901 ccaaataatt ggtggaaaga atctttcgta aattcaatag atgatagtat aaagtttttt
     6961 gacttgattc catcaaaaga aagggcaaat aaatttatat ttactgaccc aatatacact
     7021 ttgagtgcaa atatattgaa agataaaaag tcccaggata taaatagtta catggattta
     7081 aaggggaaaa cagtagtaat tccagaaggt gattattcta taaatttttt aaagcaaaaa
     7141 attcaagata taaacatttt gttgactcca gatataaaga ctggagtcaa ccatttaatg
     7201 tctggaaaag tagacgtagt tgttggtgat gaaccagtat tgcgatacta tataaataat
     7261 tatggacttt caaataaata cagtgtttta agtaatccta tatatactaa aaaagcagtt
     7321 cttgctgtac ctaaacaata tgaagagctg gttagtattt taaataaagg catttttaaa
     7381 cttcagaaaa atggagtgta taaagattta aagaaaaaat ggtattctac gtataatgaa
     7441 gttgatgata tattgtatga gcgaggaatt gtgcctagta tttatctttt tataggtata
     7501 attttaatat caatttatgt tttttatagc tatacatatt tgttaaagat agaaatcaaa
     7561 aggaaaacag aacaggtaat agaaaataaa aaaactttag aggcaacttt taatagtatt
     7621 acagatataa taatgcttgt agatgaaaat aacaatatag tagaatcgaa caaagtacta
     7681 tatgacttta tgggtgaaat gagttataag atagcagatt tgattagcat gataaaaggt
     7741 gttattgaaa ataccttctc tgaaaatact aataaaacta gtgaaataga aatacacaat
     7801 aaaattttaa agataaacac ttttcctgta gagtataaaa aaaataatac tgagtatata
     7861 gtagttctta ttaaagatat aactaatgat aaaatagtag aggcgaaatt acttagagaa
     7921 aacaaaatga tatctatagg acaattagct tctggggtag cacatgagat aagaaatcct
     7981 ttgggtataa taagaaataa ttgctatctc ttaaaagata acgttacaat ggaagaagta
     8041 aatgattgtg ttaaatcaat agaaagtaat gtagataggg caagtaatat aataaccaac
     8101 ttgttaaatt ttgcaagaat atcagatgat aatttagaac atataaatat aaaaaatttt
     8161 attgaaaata tagtgaaact tcaatataaa atgttacagt taaagaatgt tgaaataaaa
     8221 attgactgtg aacataattt aatatgctat ataaatggag aatcgttaaa acatgtattt
     8281 ataaatttaa tttctaattc aatagatgca atacatcaag atggaaaaat tattatatac
     8341 tgttatgaaa aaaatcattg tttatttata gattttaaag ataatggtga aggaataaaa
     8401 gaagatgtcc taaaggacat atttaatccc ttctatacaa caaaaccaat tggtgaggga
     8461 actggtttag gtttatatat tacttataat gagattaaaa agaataatgg agatataagt
     8521 gttgaaagta aattgggggt tggaacttgt tttcatataa aaattccatt aaataaagag
     8581 gtgactatat aatgaaaata ctattagttg atgatgagct agaatatgga gtggtaatga
     8641 aaaaaattct acaaaagaag ggctatcttg tagatatagc actaagtggt gaagaagcta
     8701 taaatataat taaaaaagat aaaaattatg atttggtttt atctgatgtt atgatgaaaa
     8761 atatggatgg agtacagcta ttagatagaa ttaaatctat taatagagat atagaggtta
     8821 tattagttac aggatatgga agtattgaaa atgcagttga tgctatgaaa agaggagctt
     8881 tatcttattt tataaaaagt aatcccattg aaaatttatt agaagaagtt gaaaaggtaa
     8941 aggcaagtaa aatatctgtt tctgtacaaa aaaataattt tgaatttact ttggaatcta
     9001 aaaatagaga ttttaataat atcataaaaa tagcaaaaaa agctgcttgt aaagatgtta
     9061 atatattaat cttgggggaa tctggtgtag gaaaagatat attagctaga tatatacata
     9121 gcataagtcc aagaaagaat gagatatttg tacctgtaaa ttgttgttct ttttcagaaa
     9181 atctattgga gtctgagctg tttggtcatg aaaaaggttc ttttacagga gcagtagata
     9241 gtagaaaagg tagatttgaa ctttcgaata aaggaactct atttttggat gaaataggag
     9301 atatacctct aaatgttcag gtaaaacttt tgaggacact tgaggacaaa tctatagaga
     9361 ggataggaag taataaatct attaaagttg actttaggtt aatttgtgct atgaataaag
     9421 aaccaaaagt ggaaatatca aatggaaata taagagaaga ttttttttat agaataagta
     9481 caataacaat aactatacca ccactaagaa agagaagaga agacttaact actcttatag
     9541 aatttttctt aaataagtac caaattgaac atgataaaaa gatacactcg attgataagg
     9601 aagtaaagga ttttttatta aattacaatt atccaggaaa tataagagaa ttaaaaaata
     9661 taataaatag acttgtagtt ttaagtgaag aaggtaattt atcaaaagac aatttaaatt
     9721 taatatcaaa taatgtatac atagatgata aaataagtat tagaccttta agagaaataa
     9781 gaaaagagtt tgaatgtgag tatattgaaa aagttttatc tttgtgtgga aataatattt
     9841 caaatacagc taaaaaattg gagattagta gaagacagtt aaccaataaa atatcagagt
     9901 ataatattaa gtgagaaaaa aacttctcat taagtgagaa aaaaatttct caaactgtat
     9961 taaaagataa acagaaaaaa tctgtttatc tttttttatt ataattttaa aaattaatat
    10021 ttacaataat tattgctaaa ttcaatattg gtgtaaatta aaaatgaaaa tttctatgac
    10081 attaatacat agaatatata ttttaaataa ttggcataga aaatgcttta tatattaata
    10141 aacgtaataa atacttttaa ttgcgattaa atatattaag ggggattttc atatgaagct
    10201 agagctaggg aatatattta taaaaaatgt tgaatttgga gaaaagacag aagtaaaaga
    10261 tggagtacta tatattagta gcgaagaaat agaaaagata gtattagaag atgaaaggat
    10321 tgtaagtgta aatgttgaac ttgcaagacc tggcgaatca ataagaatag ctccagttaa
    10381 agatgtaata gagcctagag ttaaaattgg agatgaaagc aaaatatttc ctggaattat
    10441 aaataaagtg aagactgtag gaagtggaag aacccatgta cttttaggag cttgtgttgt
    10501 aacttgtgga aatatagttg gatttcaaga aggcgtaatt gatatgagtg gtcctactgc
    10561 taaatataca cctttttcaa aaacaaataa tatatgtatt gttataaaag caaaggatgg
    10621 tatagatact catgattatg aagaagcagc tagaattgca ggattaaaaa taggtgcata
    10681 tgtaggtgag gcaggaagag aagttgagcc agatgaagtc attgtatatg agacaaagcc
    10741 attattaaaa caagtaaaag aatatccaga cctgccaaca gtagcatatg ttcacatgtt
    10801 acaatcacaa ggacttttac atgacactta ttactatggg gtggatgcta aacagatagt
    10861 gcctacattt atgtatccaa ctgaaataat ggatggagct ataatatctg gaaactgtgt
    10921 agcaccatgt gataaggtta caacattcca tcatttaaat aatccagtta tacatgactt
    10981 atataaaaga catggaaaag atttaaactt tataggagtt attttgacta atgagaacgt
    11041 attcttagtc gataaagaga gaagctctga tatggtagct aaattaatag aatttttagg
    11101 agttgatgga gttctagtga cagaagaagg ttatgggaat ccagatactg acctaatgat
    11161 gaattgtaga aaatgtagtg aagttggagc aaatgtagtt ttgataacgg atgaatttcc
    11221 tggaaaagat ggcaagagtc aatctatagc agacgctact aaggaagcag atgcagttgt
    11281 ttcttgtggt caaggaaatc tagtggttca tttcccagca atggaaaaaa ttataggaac
    11341 tttagattat gttgaaatga tgataggtgg gtataaagga tgcttaaatg aagacggaag
    11401 catggatgca gagctacaaa ttataatagc atcaactata gcaaatggct ataatcattt
    11461 gacagcaaga tattattaaa taagtatttg aatataaagt tctgttgatg taatcattgg
    11521 tctaactgat gtaataacta taaagtgatt tggaggatta agagatgagt aaaatcagag
    11581 tggttcatta tataaacaat ttctttgctg gtataggtgg agaggaaaaa gcagatattc
    11641 cgccagaaaa aagggctggt gtagtaggac ctggaatagc atttcagagt caatttaatg
    11701 atgatgcaga gataatctca actgtaattt gcggagatac gtattttggt gaaaatattg
    11761 agagtgcaac tcaagaactt ttaaaaatga taaaagaaga aagccctgat ttatttattg
    11821 caggcccagc atttaatgca ggtagatatg gagtagcttg tgggactatt tgtaaggcag
    11881 ttgaagaaga gttaaatatt cctgtaatta caggcatgta taaagaaaat ccaggtgtag
    11941 atatgtttaa attagatttg catatagtgt ctacaggaaa ttcagcagca agtcttagaa
    12001 aggtagttcc aataatgaca aaactaggtc ttaaattggt tagaggtgag gaaataggac
    12061 ctcctgaaga ggaaggatat ataatgagag gaataagaaa aaatttcttc catgaactta
    12121 gagggtctga aagagcaatt gatatgttgg ttaaaaagat gaaaggggaa agctttgaaa
    12181 ctgagtatcc aatgcctgaa tttgatagag ttacacctat gaaacctgtt aaagatttat
    12241 ctaagataaa acttgcccta gtaacttctg gagggatagt tccaattgat aatccagaca
    12301 aaattgaatc ttcaagtgct actaaatatg gaatttacga tttaacagat atggattcta
    12361 tgtcaaataa agaatttaca actatacatg gggggtatga cagagcatat gtccttgaaa
    12421 atccaaattt agttgtacca ctagatgtag ttagagagct tgaaaaagaa ggtgtaattg
    12481 gagaattagt agattatttt ataactacta caggaacagg aacgagtgtt ggcaattcta
    12541 agagatttgg agaagagttt tctaagaaat tgttagaaga tgatgtagat gctgtaattt
    12601 taacttctac atgaggtact tgtactcgtt gtggagcaac gatggtaaaa gagattgaga
    12661 gagctggtat agcagttgtt catatatgta cagtagtacc aatatcactt acaataggag
    12721 ctaatagaat tgttccagca gtagggatac catatccact tggaaatcct atgttaggag
    12781 aagaggaaag taaaaaaatt agaaagaata tagttttaaa agctttaaat gcactaacag
    12841 aagatataga agaacaaaca gtatttgaat aataaaaata caacacaaca ctgaaataaa
    12901 aaaagatatc aaaaaataat tttttaaaat tattttacga tatctttttt ttattaggta
    12961 aattcgaaat ttgcattttt ttaaattaaa tttataaaag tgataaatta gtttattata
    13021 atattgatac acaaattcaa attagatata ctatgaaaag cgagattatt acattttgga
    13081 acaaatattc aacatattag atcatattaa ttgaacagaa aaaattaact ttatataata
    13141 attagaataa tgataagaaa gaaggtgtat atattgattt caattgccat ttgtgatgat
    13201 gaatcctata tgctaaatca tttaaaggaa aatatagaat tatacatgaa gcagaagaat
    13261 ttattatata atatagagct ttttaataaa gtagaaaatt tactatgcaa tcatgaaaag
    13321 tttaatattg tatttttaga tatacagatg gaagagatta acggaatgca ggcagctaga
    13381 aaattaaggt catatggtaa tgagtgcttt ataattttcg ttacagtttt aaatgaattt
    13441 gtatttgatg cttttgaagt agatgctata aattatctga taaaacctct atctaatgag
    13501 aaactatata aaactttgga taaaatagtt acaaaacttg atgataatga aagtaattat
    13561 atcacaatac aaaaaggtta ttcatattgg aaattaaaac ttgctgatat tttatattgt
    13621 gaagttataa aaagaaagat ttatgttcat ttaaagaaag agactgtaga ttattatgag
    13681 actattgaaa atttagagag aagtttacca acaaattttt ttaggtgtca tcgtagttat
    13741 ataatcaatt tacaatatgt atatggatat gaaaatggta atgcagttat ggaaagtgga
    13801 gatagaatac ctgtttctag actacgtcaa caaatatttt caaaagttat gcttgagttc
    13861 atgaaaaata ggaggatata agatgattaa tttattttct tccgatttat ttttaaagta
    13921 cagttttttc ttctctttta ttgtatatag aattttaatg gtaatagtgc aatactattt
    13981 tctaatcaat tttacaaatt ttaattcaaa attttggtgt tttattttat attcgataat
    14041 ttcaattttt ttaatcatta taaatacata ttttaattta tataatttat tttcttcaca
    14101 gattctattt tttcttatga cttttttatt tgtaaagtac ataataaaac aatctactgt
    14161 tttatctata gtatcaactt
//
LOCUS       DBGJFF010000098        14114 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.099, whole genome shotgun sequence.
ACCESSION   DBGJFF010000098 DBGJFF010000000
VERSION     DBGJFF010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14114
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.099"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            <1..645
                     /locus_tag="ACOIAF_002806"
     CDS             <1..645
                     /locus_tag="ACOIAF_002806"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HGZ2669809.1"
                     /translation="NYIGKIPNKGNLFMKIKENVLMLDSIRGSNCFIVFDKEIILIDT
                     GLPFMGENIIKELKTLGIKLTDIKHILLTHHDVDHISNIKLLKEKTGAKVWAHVEDIP
                     FIIGEKDRPGFKKFIGKAISRRLIKDVEPYGENMQIGNIKIVHTPGHTPGHVCMIFED
                     VLFAGDLVKNKNGNIVPYPNPWNWDYKKMMKSIRELDNLKYEWICMSHGTPCIK"
     gene            1149..1898
                     /locus_tag="ACOIAF_002807"
     CDS             1149..1898
                     /locus_tag="ACOIAF_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HGZ2669810.1"
                     /translation="MRLESLVNQNYDNLNENDKIIIKQILICQREYRNFSCEKIANEC
                     NVSRATLLRLCRKIGLDSFSDLKYLLKNNQLEFVNNDNNFHDVCENYRLLMNELQKIS
                     FQDICKIIYDAETIYIYGTGNEQKSLAQEIKRIFLSVGKCVIDLFDIGEIEFMRESFQ
                     KSDLFIIISLSGETKAGIEIAKFINNHIHTISLTRLDNNTIATLCEYNLYVETQKLDT
                     LQAISYEVVGAFYALLDLLHMNYIEYRRRKE"
     gene            1895..2659
                     /locus_tag="ACOIAF_002808"
     CDS             1895..2659
                     /locus_tag="ACOIAF_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HGZ2669811.1"
                     /translation="MKIEGLFNKYYNSFTANDKYICECILNHKEDCIKLSIDEFADKY
                     HISTSALSRFAQKLQLPGYSELRVIIRLNETEVEYKNQSKHQLMDCYNKVIDDIEKKD
                     CSIMFERMKKANRIIVFGEGYSQERVAKEMKRIFLPTGKIIYDVYGYDMIQALNHFIK
                     TDDMIFFISLNGDASTIIDFAKEMRMKGIYMVSITKMISNPLARLCDENLYIQSISIS
                     VDKQLDYDVTTPYFILIELLYIKYKMYLESEHIPRK"
     gene            2758..3579
                     /locus_tag="ACOIAF_002809"
     CDS             2758..3579
                     /locus_tag="ACOIAF_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HGZ2669812.1"
                     /translation="MKLYKLLILDIDGTLRDEVYGIPESAKHAIHLCQKNHCSVVICT
                     GRSMGTIQDDVLSLGVDGYIAGGGNYIQYHGELLYNQSFNQRLIKEVVYLLKKREVAF
                     SIESQEKVFMNQKAKEIFETMNQLKGTNSCINKQHIQEKITYENNIEEYKSQDIHKIC
                     LWSNEKVFDEVKDILQDKMELAQRDISSQYYEIIQKDFHKGKAIKRLQERLGVTQKET
                     ICFGDGQNDIVMFQASDVTIAMKNSHQQLKDIATSICEDIFDNGIYKELKRRNII"
     gene            3593..5257
                     /locus_tag="ACOIAF_002810"
     CDS             3593..5257
                     /locus_tag="ACOIAF_002810"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HGZ2669813.1"
                     /translation="MEKKWWQKEIVYQIYPRSFQDSNNDGIGDLQGIISRLDYLKDLG
                     ITSLWLCPIFKSPMDDNGYDISDYLDIASEFGSMEDLKELIKESKDRGIKIILDLVLN
                     HTSDEHPWFQEALKNPESPYHDYYIFKKGNDLPNNWRSVFGGSVWEKVENRDEYYFHS
                     FGKKQPDLNWENPILRSELYQMINTWSQMGVAGFRVDAITFIKKNLSFQSLESDGVDG
                     LVKCTKTVRNQPGIEKMLYELKENTFKKYDCMTVAEAAGISYDQLGDFIGEDGFFSMV
                     FDFKHADLDVASGSEWFKRISWTIPDLRKCILDSQMYVQEHGWAANFIENHDQPRATT
                     KYLLQYERNDDAVKMLGAMYFFLRGTPFIYQGQELGMTNFKRDTINDFNDLSSIDQYY
                     RSIDEGYSEEEALEFVNLRSRDNARTPFPWADKENAGFSDTTPWLKVIENYKDINAQS
                     QINNSESILEFYKKMIKLRQDSEYSDCLIYGSIEPIDIESEDVIAYRRQLDLVVIDCY
                     YNFSDKPVEIRVEEKYDIVFQNLDDVNLLNKTVRLKPFHAVLLKAR"
     gene            5262..7130
                     /locus_tag="ACOIAF_002811"
     CDS             5262..7130
                     /locus_tag="ACOIAF_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HGZ2669814.1"
                     /translation="MNKYNKIANELIKIIGEDNIISITHCATRLRVMVKDREIINDKK
                     VEKVDEVKGVFFTSGQYQIILGTGIVNKVYAEVEKMGLKTLSKKEQDELVKNNETGFK
                     KVMRTLADIFVPIIPVIAATGLFLGLKGCLFNDNVLGLFGMSSANIPLYIQTLVSVLT
                     ETAFAFLPAIIVWSAFKVFGGTPVIGLVIGLMLVSPILPNAYSVADPSNEIEAIMAFG
                     FIPIVGCQGSVLTAIVTAFIGANLEKWFRKHMPNVLDLIFTPFFVMLITMLVILLGVG
                     PIMHTIELKMVDIISLLIDLPLGIGGFIIGFTYPLAVITGLHHTYVMIETSLLANTGF
                     NALITLCAMYGFANIGTCLAFMKKSKNNQVKQTAVGAMLSQLFGISEPVLFGIQLRYN
                     LKPLIIMCASSGLGAAILSILHIQSNSYGLAVLPSYLMYIYDGYNLITYLLVSIFVVA
                     FCFIVTCLFGVPKEAINEDEELVFNENNENFVSPAKGKIVALENVPDETFSKKMLGDG
                     FAIDIIDGKIVSPISGKLETVFSSGHAFGIKGTNGEVLIHVGIDTVALNGDGFDVAVK
                     QGDMVKQGDVLVIVDLKRIHELGKSTLTMVLFPDGKKVNILDINKDVKIGQRICVE"
     gene            complement(7485..9284)
                     /locus_tag="ACOIAF_002812"
     CDS             complement(7485..9284)
                     /locus_tag="ACOIAF_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaR1 family beta-lactam sensor/signal
                     transducer"
                     /protein_id="HGZ2669815.1"
                     /translation="MYAPFFIRFLLSTLTLSILIVVILLAKKVFKKHISAKYQYNIGF
                     ILLIMLIIPFVPLKYINLGNVFNYLYTFNQLGSSTNRTSMIKNSPNSIINNSNALNDF
                     SVSLNQVNFDSLNILLLISWIIGILIMIAITIHCNIKIKNMKKSIQILRDDEIVFIFE
                     KCKKDIGINKDFILCKSPIINTPITFGYFKPHIILPDKSISKLSLKDIKYILLHELQH
                     FKNKDILINYIMCFLQILYWFNPLVWYAFKEMRTDREIACDISVLKKLDKDSHIEYGQ
                     TLINFVDNISRPSNLTLTSDIGGSKKQIKKRLLKIMDFHNESNTLKFKSILIFTLIAL
                     IVFGNIPKLSIMADINEKYRPTNEQTVYEDLSQYFKGFDGSFVMYDLKSKQYHIYNKE
                     KSKTRISPNSTYKIYSALLGLENGIITRNNSHIPWDGKHHSNSSWNEDQDLFSAMENS
                     VNWYFQTLDKKTGLKNIQTYLRQIGYGNYDVSGGISNFWLESSLKISPIEQVKLLESF
                     YTNKFKFEDKNIETVKDSLLISKKDGISLFGKTGTANINGKDVNGWFIGYLEKNENTY
                     FFATNIQSKDYSNGSSASKITLSILNDKKIYNP"
     gene            complement(9287..9673)
                     /locus_tag="ACOIAF_002813"
     CDS             complement(9287..9673)
                     /locus_tag="ACOIAF_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HGZ2669816.1"
                     /translation="MKKLPHISEAEYQVMKIIWKYAPISTTEVIEKLVETSTWSPKTI
                     QTMLLRLVKKGALTYEKNSRVFVYTPLVKEEEYVATESSSFLNRFYNGTLNSMVLNFL
                     ENDKLSEDDIEELREILNKRTTKGDK"
     gene            9906..10583
                     /locus_tag="ACOIAF_002814"
     CDS             9906..10583
                     /locus_tag="ACOIAF_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HGZ2669817.1"
                     /translation="MIITVINGSPRKNGATSKVLTYLYKDIERLIPDVKINYFDLSEV
                     NPSYCIGCLNCYKMGKCINQNDKVEYIHDIITKSDGVIFGSPTYGSSVTGLFKVFTDR
                     AHMMLERLLYRKPCIAVTTYENARGSKAISFIKSMVLDSGGYVCGSLSIKTGFNQNPI
                     TEKVESKIQKVSKKFIYCIEEKKNPPVLSQIYNFIAINAVLKPMAFKDIEQYKGIIDR
                     WEEQGII"
     gene            10595..11020
                     /locus_tag="ACOIAF_002815"
     CDS             10595..11020
                     /locus_tag="ACOIAF_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HGZ2669818.1"
                     /translation="MKDFEKLYEATTKLVSTSLKVKDDLKKMFKQNGYDITTDHYALL
                     RFLWEQDGISQIDLCEKSCKDKSNTTRILDVMKNKGLIVRKVDVKDRRKFQIFLTDLG
                     RELEEPLNEIASIYATETFKSISDEELPLFTDILDRIGK"
     gene            11088..11867
                     /locus_tag="ACOIAF_002816"
     CDS             11088..11867
                     /locus_tag="ACOIAF_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901940.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HGZ2669819.1"
                     /translation="MNSKIYYFSGTGNSLVVSNILKETLNGSKEVIPLAIHRKKNNID
                     INEDILVIVFPVYFADVPDIVKSFVEKLNFNINTHIYAIATCNGVAGHSLFTIDKLLK
                     KRGKKLSAGFLINMPGNALITPPDVEEERLKNQTNRVAEIAKCINNLEIGNIEGKNNL
                     KSHIDSVVLRLVGKNMQIATTKFFAENKCNGCGTCKRVCPVENISIVDKKPKWGNECE
                     RCLACFHWCPKEAINVKKSFLTNRKKYHHPDITVKDIFLDK"
     gene            complement(12114..12740)
                     /locus_tag="ACOIAF_002817"
     CDS             complement(12114..12740)
                     /locus_tag="ACOIAF_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HGZ2669820.1"
                     /translation="MKINIKSKLSEFIKQNNLFDDSRIISYLPNITIETDKIKTIMIN
                     EVVPTNTNDDFYSVDKDADYLKTTIPLFDSAGIKVSNINDILDMGIYITNAVKLPKSE
                     YTITRDTIKLHMPILEEEIKLFKNLEVVMLMGDVAKKSFNMITKKHIKKNVIPSISTY
                     KLRNNELYYDNLRVFPSYIMTGGNILIEKSKFEMASEDIKKMFEIING"
     gene            complement(12883..13140)
                     /locus_tag="ACOIAF_002818"
     CDS             complement(12883..13140)
                     /locus_tag="ACOIAF_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669821.1"
                     /translation="MILNRAELFRGYSLNRFNEICNILSVNKIKYKYKVKNNAYSKKP
                     FFNEITLGNLSQKEDFSYEYLIYVHKDDYEQAESLINSKLR"
     gene            13466..13978
                     /locus_tag="ACOIAF_002819"
     CDS             13466..13978
                     /locus_tag="ACOIAF_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669822.1"
                     /translation="MKKISQEKISTHIQEVPQEKIEQWKSLYEKYKSILKVNSKSVLE
                     IIEFLKRKYVIVEETSEEFKHVVINNIKLNKPFSQKISSDKELKPIVFTIPNEGNAKK
                     LYDTQEEIYSGCKIIVGMEFETGCVFVEGSDELNDEITSFKGLDKYDINNYYLVANYI
                     RCLKKYKLID"
BASE COUNT         5313 a         1491 c         2302 g         5008 t
ORIGIN      
        1 aattatatag gtaaaatacc aaataagggg aatttattta tgaagataaa agaaaatgta
       61 ttaatgttag atagtattcg tggctcaaat tgttttattg tgtttgataa agaaattatt
      121 cttatagaca caggtcttcc ttttatgggg gaaaatataa ttaaagaatt aaaaacactt
      181 ggtattaaac ttacagatat taagcatatt ttattaactc atcatgatgt tgaccatata
      241 tctaatatta aattattgaa agaaaaaaca ggagcaaaag tatgggctca tgtagaggat
      301 atacctttta ttataggaga gaaggataga cctgggttta agaagtttat tggaaaggca
      361 atttccagaa ggctcattaa ggatgttgaa ccttatggag agaatatgca gattgggaat
      421 ataaaaattg tccatactcc tggtcatact ccagggcatg tctgcatgat ttttgaagat
      481 gtattatttg caggtgattt agtcaaaaat aagaatggaa atatagttcc ttatcctaat
      541 ccatggaatt gggattataa aaagatgatg aaatctataa gagaattgga taatttaaaa
      601 tatgaatgga tatgtatgtc tcatggaact ccttgtatta aataataaaa attaacatgg
      661 attaatagag gcgtagttga ttgtatgtat gataataatt tacagcttct ttttaatata
      721 tcatgtatat ttgtagctgt aagcatattt ataacaatac ttacaatttt gtattttaac
      781 atctataata atagttttag ctttgttaac tatgaaattt acttaaagaa gtaaatattc
      841 aagtaatagt aattattaaa taagataaaa aataaatggc agatgaatta aagtaatttc
      901 taccatggaa aacacttttg agttagaaaa aactctattt tagagtatga agagatgaag
      961 cttccgaaga aagcaatatc taaagtacat gttcgaaagg ataagtactt tttggtgcct
     1021 gcttttagca ggttattttt attttaataa aaataaaatc aaatgattat tttatcctta
     1081 ttatttaaaa ctcttcccta gaaaaagcaa ctaatgtata ttaaaataat atttagggag
     1141 tggaaaaaat gagattagaa tcgttagtta accaaaatta tgataattta aatgaaaatg
     1201 ataaaattat tattaaacaa atattgattt gtcaaagaga ataccgtaat ttttcgtgtg
     1261 aaaaaattgc aaatgaatgt aatgtttcta gagctacttt attaaggctt tgtagaaaaa
     1321 ttggattaga ttcattctct gatttaaagt atttacttaa aaataatcaa ctagaatttg
     1381 tcaataatga taataatttt catgatgttt gcgagaatta tcgactatta atgaatgaat
     1441 tacaaaaaat atcatttcaa gatatttgta aaataatata tgatgcagaa acaatttata
     1501 tttatgggac aggtaatgaa cagaaaagtt tagctcaaga aataaagaga atatttcttt
     1561 cagtaggtaa atgtgttatt gacttatttg atataggtga aattgaattt atgagagaat
     1621 catttcaaaa aagtgattta tttattatca tttctttgtc tggagaaaca aaagcaggaa
     1681 ttgaaattgc aaaatttatc aacaatcata tacatacgat ttctttaact aggcttgata
     1741 ataatacaat tgcaacactt tgtgagtata atctgtatgt tgaaacgcaa aaacttgata
     1801 cattacaagc tatatcttat gaagtcgtag gagcatttta tgctctatta gatttgcttc
     1861 atatgaatta catagagtat agaaggagaa aagaatgaaa attgaaggac ttttcaataa
     1921 atattataat tcatttacag caaatgataa gtatatctgt gagtgtattc ttaatcataa
     1981 agaagattgt attaaacttt ctatagatga atttgcagac aaataccata tatctacaag
     2041 tgcactttct cgttttgctc aaaaactaca attacctgga tatagtgaat taagagttat
     2101 tatacgctta aatgaaacag aagtagaata taaaaatcaa agtaaacacc aattaatgga
     2161 ttgttataat aaggtgatag atgatataga gaagaaggat tgttcgatta tgtttgaaag
     2221 aatgaaaaag gcaaatcgta taatcgtctt tggagaaggt tattctcagg aaagagttgc
     2281 taaagagatg aaaaggattt tcttaccaac agggaaaatt atatatgatg tttatggtta
     2341 tgatatgatt caggcactta atcattttat aaaaacagat gatatgattt tctttatttc
     2401 attgaatgga gatgcatcta caattattga ttttgctaaa gaaatgagaa tgaaaggaat
     2461 ttatatggtt tctattacaa aaatgataag taatccttta gcaagattat gtgatgaaaa
     2521 cctgtatatt caatctatat ctatttctgt agataaacaa ctggactacg atgtaacaac
     2581 accttatttt attttaattg aactgcttta tattaaatac aagatgtatt tagaaagtga
     2641 acatattccc cgaaaatgaa aatttgtgct aaaaatgaac atatttttta aaagatatca
     2701 aaatattgat atcttttttt ttgaatgcta ttatgatttt gatagtaaga aaggaagatg
     2761 aaattgtaca aattattaat acttgatata gatggtacgc ttagagatga ggtatatgga
     2821 atcccagaat ctgcaaagca tgctatacat ttatgtcaaa agaatcattg cagtgttgtt
     2881 atttgtactg gaagaagtat gggtacaata caagatgatg ttttatcatt aggagttgat
     2941 ggatatattg caggaggagg taattacatt caatatcatg gtgaattact ttataatcaa
     3001 tcttttaatc aaagattgat aaaggaagtt gtttatttat taaaaaagag agaagttgct
     3061 ttttcgattg agagtcagga aaaagttttc atgaatcaaa aagccaaaga aatatttgaa
     3121 acaatgaatc agttgaaagg aactaattct tgtataaata aacaacatat ccaagaaaaa
     3181 ataacatatg aaaataacat tgaagagtat aagtcacaag atatacataa aatttgttta
     3241 tggtcaaatg aaaaggtatt tgacgaagtg aaagatatac tacaagataa aatggaatta
     3301 gcacagagag atatttctag tcagtattat gaaattattc aaaaagattt tcataagggc
     3361 aaagcaatta agagattaca agaaagatta ggagtaactc aaaaagaaac catatgtttt
     3421 ggtgatggtc aaaatgacat tgtcatgttt caagcatcag acgttacaat tgcaatgaaa
     3481 aatagtcatc aacaacttaa agatatagcg acatctatat gtgaggacat attcgataat
     3541 ggaatttata aagaattaaa aagaagaaat atcatataaa aaggagtaat ttatggaaaa
     3601 gaaatggtgg caaaaagaaa tcgtttatca aatttatcca agaagctttc aagatagtaa
     3661 taatgatgga attggagatt tacagggaat tatttctcgt ttagattatt taaaagattt
     3721 aggtattaca tcattgtggt tatgtccaat ttttaaatca cctatggacg ataatgggta
     3781 tgatattagt gattatttgg atattgcaag tgaatttgga agtatggaag atttaaaaga
     3841 gttaattaaa gaaagtaaag atagaggaat caaaattata ttagatttag ttcttaatca
     3901 tacaagtgat gaacatcctt ggtttcaaga agcattaaaa aatccagaaa gtccttatca
     3961 tgattattat atttttaaaa aaggaaacga tttaccaaat aactggaggt ctgtttttgg
     4021 tggaagtgtt tgggaaaagg tagagaatag agacgaatat tattttcatt cttttggtaa
     4081 aaaacaacct gaccttaatt gggaaaatcc aatattaaga agtgaacttt accaaatgat
     4141 taatacctgg tctcaaatgg gagtggcagg atttagagtt gatgctatta cattcatcaa
     4201 aaagaattta agttttcaaa gtttagaaag tgatggtgtt gatggtcttg ttaaatgtac
     4261 aaaaacagtt agaaatcagc ctggaataga aaaaatgctt tatgaattaa aagaaaatac
     4321 gtttaaaaaa tatgattgta tgactgttgc tgaagcagca ggtattagct atgaccagtt
     4381 aggtgatttt attggagaag atggtttttt ttcaatggtc tttgacttta aacatgctga
     4441 cttagatgtt gcttctggta gtgagtggtt taaaagaatt tcatggacaa ttccagattt
     4501 aagaaaatgc attttagata gtcaaatgta tgttcaggaa catggatggg cagctaattt
     4561 tattgaaaat catgaccaac caagagctac aacaaagtat cttttgcaat acgaaaggaa
     4621 tgatgatgca gtaaaaatgt tgggagcaat gtatttcttt ttaagaggaa caccatttat
     4681 ttatcagggg caggaattag gaatgacaaa ttttaaaaga gataccataa atgattttaa
     4741 tgatttatct agcattgacc aatactatcg ttcaatagat gaaggttact cagaagaaga
     4801 agcgttagaa tttgtgaatt taagaagccg agataatgca agaactccat ttccatgggc
     4861 agataaagaa aatgcaggtt tttcagatac aacaccatgg ttaaaggtta ttgaaaatta
     4921 taaagatatt aatgcacaga gtcagattaa caattcagag agtattttag aattttataa
     4981 aaagatgata aagttgagac aagatagtga gtattctgat tgtttgattt atggttcaat
     5041 agaacctatt gatatagaaa gtgaagatgt tattgcgtat agaaggcaat tagatttggt
     5101 agttattgat tgttattata atttttctga taaacctgta gaaataagag tagaagaaaa
     5161 atatgatatt gtattccaaa atttagatga tgtaaatcta ttaaataaaa cagtgcgttt
     5221 gaaaccattt catgcagtat tattgaaagc gaggtagaaa aatgaataaa tataataaaa
     5281 tagcaaatga gcttattaaa attattggtg aagataatat tatttctatt acacattgtg
     5341 ccacaagatt aagagtgatg gttaaagata gagaaattat taatgataag aaagttgaaa
     5401 aagttgatga ggttaaaggg gttttcttta cttctggaca gtatcaaatt attttaggaa
     5461 caggaatagt taataaagtt tatgcagaag ttgaaaaaat gggattaaaa actttatcaa
     5521 aaaaagaaca agatgaatta gttaaaaata atgaaactgg atttaagaaa gttatgagaa
     5581 ctctagcaga tatatttgtt cctattattc cagttattgc agcaacagga ttattcttgg
     5641 gattaaaggg ttgtttgttt aatgataatg tacttggatt atttggaatg tcttctgcaa
     5701 atattccatt gtatattcag acacttgttt cggttcttac agaaacagca tttgcttttt
     5761 taccagcaat tattgtatgg tcagcattta aagtatttgg aggaacacct gttattggac
     5821 ttgttattgg acttatgtta gtttccccaa tacttcctaa tgcatattct gttgctgacc
     5881 catcaaatga aattgaagca attatggcat ttggatttat tcctattgta ggttgtcaag
     5941 gaagtgtttt aacagctatt gttacagcat ttattggagc taatcttgaa aaatggttcc
     6001 gtaaacatat gcctaatgta ttagacctta ttttcacacc attttttgtt atgctaataa
     6061 caatgttagt tattttgtta ggtgtaggcc ctataatgca tacaatagaa ttaaaaatgg
     6121 ttgatatcat cagcttattg attgatttgc ctttaggaat tggaggattt attattggat
     6181 ttacatatcc attagctgtt attactggct tacatcatac ttatgtaatg attgaaacat
     6241 cactattagc aaatacagga tttaatgccc ttattactct ttgtgcaatg tatggatttg
     6301 cgaatattgg aacatgtcta gcatttatga aaaaatctaa aaataatcaa gttaaacaaa
     6361 ctgcggttgg agcaatgcta tctcaacttt ttggtataag tgagcctgtt ttatttggga
     6421 ttcagcttcg atataattta aagcctttga ttattatgtg tgcatcttca ggtttaggag
     6481 cagcaatatt atcaatttta catattcaat ctaattcata tggattagca gttttacctt
     6541 cttatttaat gtatatctat gatggatata atttaatcac atacttatta gtatcaatat
     6601 ttgtggttgc attctgcttt attgtaacat gtttatttgg agttcctaaa gaagctataa
     6661 atgaagatga agaattagta tttaatgaaa ataatgaaaa ctttgtgtct ccagctaagg
     6721 ggaaaattgt tgcattagaa aatgtaccag atgaaacatt ttctaaaaaa atgttaggtg
     6781 atggttttgc tattgatatt atagatggaa aaattgtttc tcctatttca ggtaaattag
     6841 aaacagtatt ttcaagtgga catgcatttg ggattaaagg aactaatgga gaagtattaa
     6901 ttcatgttgg aattgataca gttgcattaa atggagatgg ctttgatgtt gctgttaaac
     6961 aaggagatat ggttaaacag ggggatgttc ttgttattgt tgatttaaag agaatacacg
     7021 aattaggaaa gtctactttg acaatggtat tgtttccaga tggtaagaag gtaaatattt
     7081 tggatataaa caaggatgta aaaataggtc aaagaatatg tgttgagtag taggtgatat
     7141 gtaatgacta caaaagaaga ttatcaagag taattcaatc tttttaaagt atgagaaata
     7201 attcataaac tctaaaacaa ttaaagaggt tgtttcaaaa tagagattgt attgcaccaa
     7261 aattgttgaa atgatgaaaa tatatttaac ttcatacaaa aaattaaata gtggcaacaa
     7321 ttgaggatgt gagggagagt tgccactatt tgtatagggt attatattat gtttttaagc
     7381 tagttttact atcttctatg tttatataat aagccttgta tatgaattta atcgataatt
     7441 aatatgaaaa ttatgtgaat tttttagaat gtacagtaaa tatattaagg attatagatt
     7501 tttttgtcat ttaatatgga taatgttatc ttagatgctg aactaccatt agaataatct
     7561 ttgctttgaa tatttgtagc aaagaaataa gtattttcat ttttttccaa ataacctata
     7621 aaccaaccat ttacatcttt tccatttata ttagcagtgc ctgttttacc aaaaagtgat
     7681 atcccatcct tttttgatat taacaatgaa tcttttactg tttctatatt tttatcttca
     7741 aatttaaact tatttgtata aaaactttca agtaatttta cctgttcaat aggtgatatt
     7801 tttaatgaag attctagcca aaagtttgag ataccacctg aaacatcata attgccatat
     7861 ccaatttgcc taagataagt ttgtatgttt tttagccctg tttttttatc tagagtctga
     7921 aagtaccagt ttacggagtt ttccatagca gaaaataagt cttggtcttc attccaagaa
     7981 ctattagaat gatgctttcc atcccaaggg atatgtgaat tatttcttgt tattatccca
     8041 ttttccaatc ccaataaggc actataaatc ttataagtag aatttggaga aattcttgtt
     8101 ttactttttt ctttattgta aatatgatat tgcttggatt ttaaatcata cataacaaaa
     8161 cttccatcaa aacctttaaa atactgactt aagtcctcgt acacagtttg ttcatttgta
     8221 ggtctatatt tttcgttgat atctgccata atagatagtt tgggaatatt tccaaataca
     8281 attaaagcta tcaatgtaaa aattaaaatg cttttgaatt ttaacgtatt tgattcatta
     8341 tgaaaatcca ttatcttcaa tagtcgcttt tttatttgtt tttttgaccc accaatatct
     8401 gatgtaagtg taagatttga tggtcttgaa atattatcca caaagtttat tagagtttgt
     8461 ccatattcta tatgactatc tttatcaagc ttttttaaaa ctgaaatatc acatgcaatt
     8521 tcacggtctg tacgcatttc tttaaatgca taccatacaa gaggattaaa ccaatataat
     8581 atttgaagga agcacattat ataatttatc agtatatctt tatttttaaa atgttgtaat
     8641 tcatgaagta agatatattt tatatctttt aaagataatt tagatataga cttatctggt
     8701 aatataatgt gtggtttaaa gtatccaaat gtaataggtg tatttataat aggtgatttg
     8761 catagtataa aatccttatt tataccaata tcttttttac acttttcaaa tataaatact
     8821 atctcatcat ctcttaaaat ttgtattgat tttttcatat tttttatttt aatgttgcaa
     8881 tgaatagtaa tagctatcat gattaaaatt ccaattatcc atgatattag taataaaata
     8941 tttaaactat caaaattaac ttggtttagg gatactgaaa aatcgtttaa tgcatttgag
     9001 ttattaatta tagaatttgg actatttttt atcatagaag tcctattagt actgcttcca
     9061 agttgattaa aagtatataa atagttaaat acatttccaa gattaatata tttaagaggg
     9121 acaaatggaa ttattaacat tataagcaat ataaatccaa tattgtattg gtactttgca
     9181 gaaatatgtt ttttaaatac ttttttggca agtaaaatta caacaattaa tattgataag
     9241 gttaatgtac tgagtaaaaa tcgtataaag aatggggcat acatatttat ttatccccct
     9301 tagtcgtcct tttatttaag atttctctca gttcctcaat atcatcttca gatagtttat
     9361 cattttctaa aaagtttagt accatagaat taagagtacc attataaaaa cgatttaaaa
     9421 aagaactact ttctgtagct acgtattctt cttctttaac aagtggagta taaacgaata
     9481 ctctgctatt tttttcatat gttaaagctc cttttttaac taatctcaat aacattgttt
     9541 gaattgtttt aggactccat gtacttgttt ctaccaactt ttcaattact tcagtagtgc
     9601 tgataggagc atatttccaa ataattttca taacttgata ttctgcttct gaaatatgtg
     9661 gtaacttttt catactatac ctccttctac ttacatttgt aatacataaa gtatattatc
     9721 attaatttag tttgtcaatt attaaaaata attcatttat agttgattaa gtaagcacat
     9781 gtagttaaat aaaactttta tttaagctgt gtaagtatag ctttaaataa atggtttata
     9841 ttgacagcta aaaatatagt tgatatactt attgttgata taacaactaa aaaggagata
     9901 tagaaatgat aattacagtt ataaatggaa gccctagaaa aaatggagct acatcaaaag
     9961 ttttaactta cttgtataaa gatatagaaa gattaatacc agatgtaaaa attaattatt
    10021 ttgatttaag tgaggtaaat ccatcatatt gtattggttg tttaaattgt tataaaatgg
    10081 gcaagtgtat caatcaaaat gataaagtag agtatattca tgatataatt actaagagtg
    10141 atggtgttat atttggtagc ccaacttatg gaagtagtgt aactggactt ttcaaggtgt
    10201 ttacagatag agcacatatg atgcttgaaa gattattgta tcgtaagcca tgtatagcag
    10261 ttactacata tgaaaatgca cgtggttcaa aagcaatttc ttttataaaa tccatggtct
    10321 tagattcggg aggttatgtt tgtggtagtt tatctataaa aactggattt aatcaaaatc
    10381 ctataactga aaaagtagaa tcaaagatac aaaaggtatc taaaaaattt atttattgca
    10441 tagaggaaaa gaaaaatcca cctgtgcttt ctcaaatcta taattttatt gctataaatg
    10501 ctgttttaaa gccaatggct tttaaagata ttgagcaata taagggaata attgatagat
    10561 gggaggagca agggattatt taaatggagg taaaatgaaa gattttgaga aactatatga
    10621 agctacaact aagttagtaa gtacttcgtt aaaagtaaaa gatgatttaa aaaagatgtt
    10681 taaacaaaat ggatatgata taacaactga ccattatgcg ttattaaggt ttctatggga
    10741 gcaagatgga atttctcaga tagatttatg tgaaaaatca tgtaaggata aatctaatac
    10801 gacaagaatc ttagatgtta tgaaaaataa gggattaatt gtccgtaaag tagatgttaa
    10861 agatagacgt aagtttcaaa tattcctaac agatttaggt agagaattag aagaaccatt
    10921 aaatgaaatt gcaagtatat atgcaacaga gacatttaaa tctatctctg atgaagaatt
    10981 gcctttgttt actgatattt tggatagaat tggaaagtaa atataaatcc tattttaaga
    11041 gactttttaa ctattgatga tgtagaaata tagaaagagg gataagaatg aatagtaaga
    11101 tttattattt ttctggaaca ggaaattcgc tagtagtttc aaatatttta aaagaaacgt
    11161 taaatggaag taaagaagtt atacccttag ctatacatag aaaaaaaaat aatatagaca
    11221 taaatgaaga tatattggta attgtttttc ctgtttattt tgctgatgtt cctgatatag
    11281 taaagtcttt tgtagagaaa ttaaatttta atattaatac tcatatatat gccattgcca
    11341 cttgtaatgg ggtagctgga catagtttat ttactataga taagttactt aaaaaaaggg
    11401 ggaaaaaatt atctgctggt tttttaatca atatgcctgg aaatgctttg ataacacctc
    11461 cagatgttga agaagaaaga ttgaaaaatc agactaatag ggtagctgag attgcaaaat
    11521 gtatcaataa cttggagatt ggaaatatag aaggaaaaaa caatttaaag tcacatatag
    11581 atagtgttgt acttcgttta gtaggtaaga atatgcaaat tgcaactact aaattttttg
    11641 cagaaaataa atgtaatgga tgtgggacat gtaaaagggt ttgtcctgta gagaatataa
    11701 gtatagttga taaaaagcca aagtggggta atgagtgtga aagatgccta gcttgttttc
    11761 actggtgtcc aaaagaggct attaatgtaa aaaaatcttt tttaacgaat aggaagaagt
    11821 atcaccatcc agatattact gtaaaagata tatttttaga taaatagata tatttgactt
    11881 gtattaataa ttgttttatt aaataaaaac gttagagata taaaagaaat ataaagtaat
    11941 cttgtaaaga gtaatgttac ctagactaaa tggaatagag attaaaaaga acaatataaa
    12001 taataaatta tgttaatagt atatagttac actaatataa aataaaagtg cctcataaat
    12061 agaagttttt gaggcacttt ttattttata ttagttttta ttattttgta atatcaaccg
    12121 tttataatct cgaacatttt ttttatatct tctgatgcca tttcaaattt tgatttttca
    12181 atcagtatgt ttccaccagt cataatatat gatggaaata ctcttaaatt atcataataa
    12241 agttcattat tacgaagttt atatgttgaa atggaaggga tgacattctt ttttatatgc
    12301 tttttagtaa tcatattaaa agattttttt gcaacatctc ccattaacat tactacctct
    12361 aggtttttaa ataatttaat ttcttcttct aaaataggca tatgtaattt aattgtatca
    12421 cgagttattg tatattctga tttaggaagt tttactgcat tagttatata gatacccata
    12481 tctaggatat catttatgtt agatactttt attcctgcac tgtcaaataa aggaatagta
    12541 gtttttaaat aatcagcatc tttatctaca ctataaaaat cgtcattggt gttagttggg
    12601 acaacctcat taatcattat agtttttatt ttgtctgttt ctattgttat attaggaagg
    12661 taggatatta ttctgctgtc atcaaaaaga ttgttctgtt taataaattc acttaacttt
    12721 gattttatat ttattttcat ataacgagtc tccttagttt taagctttaa attactataa
    12781 aaagctagtt ttaaagcaca caattttgaa aatatattag catatttgaa agtataataa
    12841 catataaaac atgacaacag aatgtcatgt tttataaata atctatcgca acttagaatt
    12901 tattagagac tctgcctgtt cataatcgtc tttatgaaca taaattaagt actcataaga
    12961 gaaatcttcc ttttggctta aattgccaag tgttatttca ttaaaaaaag gttttttaga
    13021 gtaagcatta tttttcactt tatacttata tttaatttta tttactgata atatattgca
    13081 tatctcgtta aatctattta aagaatatcc tcggaaaagc tcagctctat ttaaaatcat
    13141 aatgaaaacc tccttttaaa agtaatatat tgaaaagcat aggagaaatg tactataagt
    13201 tgttataaat aaaaggtagc aaatttaaat tgatttgtaa agtttttaag agtattatat
    13261 tagtgataaa ataaataatt tttataatct aactaataac aatttaaaat aagtaactta
    13321 tgtaagaatc gtaggaacta cggagatatc ctgtagagaa ttaataagac atatttaagt
    13381 atgcaaaatt ctattaagta gaaaccttgt aactttagtc atgtgagatt cataagaata
    13441 tttaagtatt gaaaggagag tttaattgaa aaaaatatct caagaaaaaa taagtacaca
    13501 tatccaagaa gtaccacagg aaaaaattga acagtggaaa tctttatatg aaaaatataa
    13561 aagtatttta aaagtaaatt ctaagtctgt tttagaaata atagagtttt taaagcgaaa
    13621 atatgtaata gtagaagaaa cttcagagga atttaaacat gtagttatca ataatattaa
    13681 gttaaacaag cctttttcac aaaagatttc cagtgataaa gaattaaaac ctattgtatt
    13741 tacaattcca aatgaaggaa atgcaaaaaa attgtacgat actcaagagg aaatttatag
    13801 tggctgtaag attattgtag gtatggaatt tgaaacaggt tgtgtttttg tagaaggtag
    13861 tgatgagctt aatgatgaaa taacttcttt taaaggatta gataaatatg atataaataa
    13921 ttattattta gtagcaaact atattagatg tctaaagaaa tataaattaa tagattagtg
    13981 tttgattaat aaattagtgt ttgatttaag gattatagag taaatttgaa gttagtgtga
    14041 taatgaaact aagcttaaat ctaatataac tgtaaaacat aaacttaaat agagttgcca
    14101 aaataaaatt ggta
//
LOCUS       DBGJFF010000099        13943 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.100, whole genome shotgun sequence.
ACCESSION   DBGJFF010000099 DBGJFF010000000
VERSION     DBGJFF010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13943
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.100"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            <1..532
                     /locus_tag="ACOIAF_002820"
     CDS             <1..532
                     /locus_tag="ACOIAF_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HGZ2669823.1"
                     /translation="VTESLIIFYPKNSLESKSDYLLILGASVKKTTPSTTLKGRLDTA
                     LKYLKINDNCYVVVSGGKGSGEKITEAKAMKDYLIKNGIDKNRIIEEDKSTNTYENFK
                     YSKLKIEEHSQRKLSDLKVKIVTTDFHVLRSKILAYRNGYKNTSFYASKSKLSFVPTY
                     YTREFFALWKTIIFDR"
     gene            complement(481..789)
                     /locus_tag="ACOIAF_002821"
     CDS             complement(481..789)
                     /locus_tag="ACOIAF_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HGZ2669824.1"
                     /translation="MNFLKENSTFYFATVEDNKPRVRPFGFIMDYEGKLYFGIVKYKT
                     SYKQLLVNPNVEISTTSKVGKWIRIKRIEKTRYEFISSLFIYYLSKMIVFHNAKNSLV
                     "
     gene            1589..5731
                     /locus_tag="ACOIAF_002822"
     CDS             1589..5731
                     /locus_tag="ACOIAF_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HGZ2669825.1"
                     /translation="MATVSGQIVFDRNRSATIDSGDSGIANIPVVLQDIATGVRLVVL
                     TDASGNYVFTNVPNGNYRIVEAFGTTGGVPTPGNFATAIVGPVPIAEMPPISSVTNPP
                     IGSTNLDGTTPNTLLITVAGSDITNQNILNGPVIYTPIENILDSCVSVSNINLITDAD
                     NGTFGFFPPGTPSNTGPEIEPYPNVTPDFTYVVPDPTKFTPLDGEYTVQNIMTNAMSN
                     VIGAWWRIADHTTGNETGRMMVVNGFNPGAVFFKSTVQVTPNTNYLFSTWILNLFKVT
                     GYPPPQLGVRIFDQNNNILYQATLGAQIPVNTNAPEWKEVGTVINSQNNNQLTVEFFS
                     EGESAIGNDYAIDDVSLREIAVPTFTPIKTSNTETANVGDIITYTVTLDNTCDSPLTN
                     VFFRDLIPNGLEFIPGTVTVDGVSVPGVDPNTGFPLPDIGGGTGVEVTFDVVVESIPN
                     PNPTNNIANIDYSYTPVEGGIPNDFSVDSNPVPVEVISADIEVTKLSEPTIVNPGEEL
                     IYTIKVVNNGPFPSENVVLTDDVPASIINPEYSLDGGVNFQPWTGSLNIGTLEVGETR
                     VIIIRGIVNPSTVGIITNTAVVSSTTADPNLNNNASTIETEVNLLADILVMKTAEPNS
                     AVPGTLLRYTIQVENLGPANAENVILNDDIPASIINPEYSLDGGASFQPWNGSLNIGT
                     LNSGISLTVLIQGTVSLNSSEYIVNTATVSSTTPDPDLSNNISTIITPVNPQAGISII
                     KVADEDVAVPGEEFVYTIEIFNEGPSNATNVVLTDDIPDVILNPEYSLDGGATFQPWT
                     GSLNIGTVAPGQLIRIIIKGLVSSTATGDITNTAEVSADVPEPVTDSSTVTTPIVPSA
                     DIEVIKTSNMDTAVPGETFSYTITVTNLGPSAAQSIVLTDDIPDVILNPEYSLDGGVT
                     FQPWNGNLSIGTLDAGEIRSIIIRGTVSQTAVGTIINTATVSSPTPDPNPDNNTSTDE
                     TDISPLADISVIKGNEPVAIPGGRFIYGIEIANAGPSFAENVTLTDNIPASILNPEYS
                     IDNGVTFQPWNGSLNIGILDAGEIRNIIIRGTVSQTAIGTIINTATVSSTTPDPNLNN
                     NTSTSEAEVSSSADISVVKRSNQIVVVPGDVLDYTIEVRNAGPSTAQNVTLTDNIPAS
                     ILSPEYSIDNGVTFQPWNGSLSIGTLDAGEIRNIIIRGIVSQTAIGTIINTATVSSTT
                     PDPNPDNNTSTSEIDISSSADISVIKLANKTEACVGEQIDFVIVVSNAGPENAQNVTL
                     IDNVSDKLKKAVFSLDRGVSFQPWTGSLNIGTLPAGTLRVIILRGIIKSTCLDRLTNI
                     AEVTSTTPDSNLNNNISRVQVEIKQCCCNDCCCNNCSNYCCNDCGCKDCCCNCCKNDF
                     WC"
     gene            complement(6140..7333)
                     /locus_tag="ACOIAF_002823"
     CDS             complement(6140..7333)
                     /locus_tag="ACOIAF_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGZ2669826.1"
                     /translation="MKEKRRDSKGRILHTGESQRTDGKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKRDKLSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRANVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDCVRKNPFDFKLSEVLENDTKEKIALTEEQEQALLSFIKTDNVYHKYYDDVLI
                     LLKTGLRISELCGLTVTDIDLKNEVVIIDHQLLKSKEQGYYIETPKTKSGTRQVPLSR
                     ETIQAFQRVMKKRPKAEPFVIDGRGNFLFVNLKGKPKVAIDYSTLFVRMVKKYNKHHM
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(7412..7615)
                     /locus_tag="ACOIAF_002824"
     CDS             complement(7412..7615)
                     /locus_tag="ACOIAF_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006730246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HGZ2669827.1"
                     /translation="MNNNDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWLI
                     MNGNRIQIKRRQFEKVIDKLDAI"
     gene            complement(7615..8676)
                     /locus_tag="ACOIAF_002825"
     CDS             complement(7615..8676)
                     /locus_tag="ACOIAF_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HGZ2669828.1"
                     /translation="MDYISIVFETATAEDVITHILELPTDIFNVYPATVKFKTYQIRW
                     EIGDIYVSGDARKTEDNPQGLGCYLVMTGRGCDDIFRILNSRNYTFGDMFKHCERRYG
                     LDNFHFTRLDIAIDDKNEKPFFTIEQIKKKCEKEEFISNSEGYHFDESKFDDFDTAKT
                     VYIGAGKSGLSYRFYDKDKEVCSKYNKTLDEVGSWKRTEMQLRDDKAHVFAMTFKDRP
                     LELGELAFGLLANNLRFVVANRNESNKSRWKTCRFWERFLGAVEVLKLQVPKPHNSLE
                     ETQQWLTAGGVISAVKSFYFLEEHDALGGLEKVGTMLDKARYSTSLSSKLTAHLQRIN
                     RTDLIPYIQYDTKHGKGGI"
     gene            9163..9798
                     /locus_tag="ACOIAF_002826"
     CDS             9163..9798
                     /locus_tag="ACOIAF_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGZ2669829.1"
                     /translation="MEHYEKKISFLGENIQTIRKHRGMKQQELADKIGINMQSLSKIE
                     RGVNFPTFDTLEKIIDVLGVTPNELLSGEWKFVNQGEKEVCQFLKIEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIVASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIVGHKYRTPHQVVKMINPNSKEDMELLREVLKNNHFDDEDE"
     gene            10095..10757
                     /locus_tag="ACOIAF_002827"
     CDS             10095..10757
                     /locus_tag="ACOIAF_002827"
                     /inference="COORDINATES: protein motif:HMM:NF015123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HGZ2669830.1"
                     /translation="MTIVHSRDIIFVKGVDHMNITKQKSQKTFDKQAADYDTNIQGEH
                     ARKLYKPIIENLKNKNIHSILDLGCGTGALLKKIKELNIAEQLFGIDISPNMLEIARN
                     KLGNDATLILGDSERLPFEDNSFDAIVCNDSFHHYPLPDIVAKEVSRCLKQNGVFIIG
                     DCWQPIGARQIMNYYMKHSNSGDVKIYSKKEMLLLLSKDFHEIEWKSFGTTSCLIIAY
                     NN"
     gene            10781..11341
                     /locus_tag="ACOIAF_002828"
     CDS             10781..11341
                     /locus_tag="ACOIAF_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008728836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HGZ2669831.1"
                     /translation="MRISKEPEERKQEILETAIKLFSVNGFEKTSISDIAKEIGIAQG
                     LCYRYFPSKDVLFQSAINEYADILVANMTKNINIKKDSLKDILRKMILFTEQEDDTYY
                     PVFHNNQNKNFHNLLTLNVCEKLFPIVSEIIERANCTNEIQVNDVEMYASFCIYGQLG
                     ILLNTDISTKEKSTRIKAFFRDLFRL"
     gene            11417..12769
                     /locus_tag="ACOIAF_002829"
     CDS             11417..12769
                     /locus_tag="ACOIAF_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005363205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HGZ2669832.1"
                     /translation="MNKNIDFVNGNTKKCLIEMTVPMIIAMFLNMTYNLVDSLWIGNL
                     LGEQAYAALTNSTPIILILTSIGMGATNGLAILLSQVIGAKNEQKKKNIIATSFFVSV
                     ILSVLITIVLEIFLEPILIVLNTPTEIFDMAKNYLSIYVLGYVAVYLYLYFTAVLRSF
                     GNTMFQAVAMLVSTLLNAILDPIFINIIGFHGAAIATLISQIICLAFMCIYIYRKKLF
                     NLSLSDFDTTEIMPFINNAVPSVIQQSIPAISTTFLTAIVSTYSISAIAGYGVAGKLE
                     MILFYPAMALNMVLTSIVGQCIGAQRIDRAKNYLKLALKYGIIVSIVLSAVVIFFSKQ
                     LAKLFVSNEAVTNVVSHYFLIVSIGYILYTITSCYLGTLNGMGKPTKSMVLMIFYYII
                     IRIPLAYLLSWLETDLNGVWIAILISHIVACIITILTSNQIIKRLYGRFYDRTKKSKI
                     "
     gene            13015..13434
                     /locus_tag="ACOIAF_002830"
     CDS             13015..13434
                     /locus_tag="ACOIAF_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HGZ2669833.1"
                     /translation="MNTSSFEHIVRIQFNALMMTVIKCTVKSRNKQFARRSKREVLFC
                     ELSDMKMFEQGAEDNYSCNYTSFEVLNFTIQISNEKLAVALYKLSNKERDVILLRYFQ
                     SISDQEIAELYHVSRSTVYRRRSNGLKRLKTLLKERN"
     gene            13439..13678
                     /locus_tag="ACOIAF_002831"
     CDS             13439..13678
                     /locus_tag="ACOIAF_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGZ2669834.1"
                     /translation="MRKEYGYPELEVICKASAGNEASIKEILNFYDAYICKLCLRPFY
                     HSESGKITMQIDEELKGQIHTEMMKAILKFEIRVK"
BASE COUNT         4819 a         2195 c         2436 g         4493 t
ORIGIN      
        1 agttactgag agtctaatta ttttttatcc caaaaatagt ttagaatcaa aaagtgatta
       61 tctcttaatt ttaggagctt ctgtaaaaaa gactacacca agtacaaccc tgaagggtag
      121 gttagacact gcattgaaat acctaaaaat taatgataat tgttatgtgg tagtatctgg
      181 tggtaaagga agtggagaaa aaataactga ggcaaaagct atgaaagatt atcttataaa
      241 aaatggtata gataaaaata ggataataga agaggataaa tccacgaata cttatgagaa
      301 ttttaaatat tcaaaattga agatagaaga acatagtcaa agaaaactaa gcgatttaaa
      361 agtaaaaata gtaacaacag attttcatgt tctcagaagt aaaatattag catatagaaa
      421 tggatataaa aatacgtctt tttatgctag taaatcaaaa ctttcatttg taccaactta
      481 ctatacaaga gaattttttg cattatggaa aacaatcatt tttgatagat aataaataaa
      541 aagactagat ataaactcat atctagtctt ttctattctc ttaattctaa tccattttcc
      601 aactttactt gttgtagaaa tttcaacatt gggatttaca agcaattgct tataagaagt
      661 tttatatttt acgattccaa aatataattt tccttcataa tccattataa atccgaatgg
      721 tcttactctt ggtttattat cttcaactgt agcaaaatag aatgttgagt tttcttttaa
      781 aaaattcatt attttttcat gattaaatcc tctttgtatc ttatactaat ataatgaata
      841 taaataaaat atttataata ctaatataaa tcgatataaa aacaattaca atattatatt
      901 gattattcaa aattatatat aaaattcaga aaaaaattag tttacataat aaaaataaag
      961 taaactaaaa ttattgattt tagattaaag taccagatta agcatttaag tagattttaa
     1021 gcttgatttg attagaaagg tacaaactat caccgaaaaa aattaaaccc atttaaaaag
     1081 caatataaaa gcttgttatt tttgagaaaa atagaccctc taaactcaat tagataaaaa
     1141 taaaatacag aaaattatat ctctaaatat cttaaataat taagtttaaa ttttacgata
     1201 ttttgtttga tgatttataa tataaaagca tactcttaat cttcaaaatt taaaaagagt
     1261 aaaaaataac aaaaaaagca ctgacatttc actgacaaat tgtaattttg ttattataaa
     1321 tacattgaaa atacttgatt attgaaaaat ggcataaaat aatacatttt atgccattta
     1381 ctattatttg atagttttta acttatgaca taagatattt tttaacatat acaataaaat
     1441 ataaaaaaat tgatattgca aataaaaaaa atacatatta gatgttatag aaaaaaagcg
     1501 cacttatagg cttaaaatgt cacataaaag agttttaaag aataatatat tatgaacaca
     1561 aattttgtaa tcacaaagga gtgataatat ggcaacagta tcaggacaaa tagtttttga
     1621 tagaaataga agtgctacca ttgattctgg agactcagga atagccaata taccagttgt
     1681 attacaagat atagcaactg gagttagact tgtggttctc acagatgcat caggaaatta
     1741 tgtttttact aatgttccaa atggtaacta tagaattgta gaagcttttg gaactacagg
     1801 aggagtacca acaccaggga attttgcaac agcgatagta ggtccagttc caattgcaga
     1861 aatgccacct attagttctg tgacaaatcc tcctattggt tcaacaaact tagatggtac
     1921 aacacctaac acactattaa taacagtagc aggttcagat ataacaaatc aaaatatatt
     1981 aaatggacct gtgatttata cacctataga gaatatattg gattcttgcg tatctgtatc
     2041 taatataaac cttattacag atgcagataa cggaacattc ggtttttttc caccaggtac
     2101 accaagtaat acaggacctg aaatagaacc ttacccaaat gtgacgccag attttacgta
     2161 tgttgtacca gacccaacta agtttacacc tttagatgga gaatatacag tacaaaatat
     2221 tatgacaaat gcaatgagca atgttatagg agcttggtgg cgtattgctg accatactac
     2281 aggaaatgaa acaggaagaa tgatggtggt aaatggtttt aatcctggag ctgtattttt
     2341 taaatcaaca gtacaagtta cacctaacac taattattta tttagcactt ggattttaaa
     2401 cctttttaag gtaacaggat atccaccacc acagctaggt gtaagaatat ttgaccaaaa
     2461 taataatata ctatatcaag caacattagg agctcaaatt ccagttaata caaacgcacc
     2521 agaatggaaa gaagtaggta ctgtgattaa ttctcaaaat aataatcaac ttacagtaga
     2581 attttttagt gaaggagaaa gcgccattgg aaatgactat gctatagatg atgtttcttt
     2641 aagagaaata gctgttccaa cttttactcc aataaaaaca agtaacacag aaactgctaa
     2701 tgtaggtgat ataatcacat atacagttac attagataat acttgtgata gccctttaac
     2761 taatgtattt tttagggatt taattccaaa tggtttagag tttatacctg gaactgtaac
     2821 ggttgatgga gtatctgtgc caggagtaga ccctaataca ggtttcccac ttccagatat
     2881 tggaggagga acaggggttg aagtaacttt tgatgttgtg gttgaaagta ttcctaaccc
     2941 aaatcctact aataatatag ctaatataga ttactcctat acacctgtag aaggcggtat
     3001 accaaatgat ttttcagtag attctaatcc agttcctgtt gaagtaatat cagcagatat
     3061 tgaagttaca aaattatctg aacctactat agtgaatcca ggagaggaat taatatacac
     3121 tataaaagta gtaaataatg gaccattccc atctgagaat gtggttttaa cagatgatgt
     3181 accagcttct attataaatc cagagtattc attagatgga ggagtaaatt ttcaaccatg
     3241 gactggaagt ttaaatatag ggacattaga agtaggagag acaagagtta ttataattag
     3301 gggtattgta aatccatcaa cagtaggaat tataacaaat acagctgtgg tatcttcaac
     3361 tacagcagac ccaaacctta ataataatgc atctacaata gagacagaag tgaatttatt
     3421 agcagatatt ttagtaatga aaacagcaga acctaattca gctgttccag gtactttatt
     3481 gagatatact attcaagtag aaaacttagg accagcaaat gctgaaaatg taatattaaa
     3541 tgatgatatt ccagcttcta ttataaatcc agaatattca ttggatggag gagcaagttt
     3601 tcaaccatgg aatggaagtc ttaatatagg aactttaaac tctggaattt cattaacagt
     3661 tttaattcaa ggaacagtta gtttaaattc ttcagaatat atagtaaata cagcaacagt
     3721 ttcttcaact acacctgacc cagatttaag caacaatata tctaccatta ttacaccagt
     3781 taacccacaa gcaggtattt ctattataaa agtagctgac gaggatgtag ctgttccagg
     3841 agaagaattt gtatatacaa ttgaaatttt taatgaagga ccatctaatg ctacgaatgt
     3901 agttttaaca gatgatatac cagatgttat tttaaatcca gaatattcat tggatggagg
     3961 agcaactttt caaccatgga ctggaagtct taatataggt acagttgcac cagggcaatt
     4021 aataagaatt atcataaagg gattagtaag ttcaactgct acaggggata ttactaatac
     4081 agcagaagta agtgcagatg taccagaacc agttacggat tcatcaacag tgacaactcc
     4141 tatagttcca tcagcagata tagaagtaat aaaaactagc aatatggata cagcagttcc
     4201 aggagaaact tttagttata ctataacagt tacaaattta ggaccatctg ctgcacaaag
     4261 tatcgttttg acagatgata taccagatgt tattttaaat ccagagtatt cattggatgg
     4321 aggagtaact ttccaaccat ggaatggaaa tcttagtata ggcactttgg atgctggaga
     4381 aataagaagt attataataa gaggaacagt gagccagaca gcagtaggta caattataaa
     4441 tacagcaaca gtaagttcac ctacacctga cccaaatcca gataataata cttcaacaga
     4501 tgaaacagat ataagtccat tagcagatat ttctgtgata aaaggcaacg aaccagttgc
     4561 tataccaggg ggaagattta tttatggaat tgaaattgca aatgcaggac catcttttgc
     4621 agagaacgta actttaacag ataatatacc agcttctatt ttaaatccag agtactcaat
     4681 agataatgga gtgactttcc aaccatggaa tggaagcctt aatataggta ttttagatgc
     4741 tggagagata agaaatatca taataagggg aacagtaagt caaacagcaa taggcacaat
     4801 tataaataca gcaacagtaa gttcaactac acctgaccca aatctaaata acaatacttc
     4861 aacatcagaa gcagaggtaa gttcatcagc agatatttct gtagtaaaac gaagcaatca
     4921 gattgttgtt gtaccaggag atgtactaga ctatacaatt gaggtgagaa atgcaggacc
     4981 gtctactgct cagaacgtaa ctttaacaga taatatacca gcttctatct taagtccaga
     5041 atattcaata gataatggag tgacttttca accatggaat ggaagtctta gtataggcac
     5101 tttagatgct ggagagataa gaaatattat aataagggga atagtaagtc aaacagcaat
     5161 aggtacaatt ataaatacag caacagtaag ttcaactaca cctgacccaa atccagataa
     5221 taatacttca acatcagaaa tagatataag ttcatcagca gatatttctg taataaaatt
     5281 agcaaataaa acagaagcat gtgtaggaga gcaaatagat tttgtaatag tagtatcaaa
     5341 tgcaggacca gaaaatgctc aaaatgttac tttaatagac aatgtttcag ataagcttaa
     5401 gaaagcagta ttttcacttg atagaggtgt aagtttccaa ccatggactg gaagtcttaa
     5461 tataggtaca ctaccagctg gaactttaag agtgattata ttaagaggaa ttataaaatc
     5521 aacatgtctt gatagattaa caaatattgc agaagttact tcaacaacac cagattcaaa
     5581 ccttaacaat aatatatcaa gagtgcaagt agaaataaaa caatgttgtt gtaatgattg
     5641 ctgttgtaac aactgtagta attattgttg caatgattgt ggttgtaaag attgttgctg
     5701 taattgttgt aaaaatgact tttggtgttg attttataaa ataattttgt ataccttaaa
     5761 ttaaactgaa ataaaaaata aattcacata tgagtaaaat attttataaa tattaataaa
     5821 ttttctatct ttgatatgga ccacattcgt aatactagtt attaaatgaa gtggtccttt
     5881 ttaattcatt aaaattattt attctaatta aattttaaat gtattaaata attttaattt
     5941 tttataaaaa atattgatat gttatttgtc agaaattata tatgtctttt taactatctt
     6001 tttatatctt ctcaaatgtc cgcaaagcct tattttatcg gctctacggg cattttgcct
     6061 ttgtggtaaa cctcacatat ctaggcttat cttcttatat tttccctgtc aatcgtggtt
     6121 aaaatcgtgg taaatacttc tatgcaatta gacgctgaac ctctgatttt gcggtatcta
     6181 tggacgcatg agcataccag ttcattgtga tactgatatt tgaatgcccc atgatatact
     6241 gtaaatcttt cgggttcatg ttcttgcttg ccagtcttgt gcagaacgta tggcgaagcg
     6301 tatgcggtgt gatatgtggc aaagggttgt ccatgtggtg cttgttgtat ttctttacca
     6361 tacggacaaa taaagtgctg taatcaatcg caacttttgg cttgcctttg agattgacaa
     6421 ataagaagtt gccccgtccg tctatcacaa atggttctgc ctttgggcgt ttcttcataa
     6481 cccgttgaaa tgcctgtatc gtttctctgc ttaatggcac ttgcctagtt ccgctctttg
     6541 tcttaggcgt ttcaatgtaa taaccctgtt ccttgctctt taataactgg tggtcgataa
     6601 tcacgacttc attctttaag tcaatatcag ttaccgtcag tccgcacagt tccgagatac
     6661 gaagtcctgt ctttaacagt atcagtacat catcataata cttgtgatac acattgtccg
     6721 tcttgatgaa tgagagtaag gcttgttcct gttcctctgt caatgcgatt ttctctttgg
     6781 tatcattttc taggacttca cttaacttga aatcaaaagg gtttttcctt acacaatcgt
     6841 cttgtatggc gatatagaat gacgctttta acgagcgttt atggttgtta atggtgttat
     6901 aggaaaagcc tttgtctttc atgcgtaacg cccattcctt agcgtcagag ggttttatcg
     6961 tatcaatgct cctagcacct aacttgtcct cttttaataa ccgcataagt tgttcccgct
     7021 gtttttgtgt gcttttcttc acgtttgccc tctgtgcgtt ctgtttggcg tagagttggc
     7081 aaagcgtcat tttcttacct gtgctgtcga taccgtcctc aatatcccgt cttatctgct
     7141 gttccagttc acgaagtgag agtttatccc gttttccctt tggtgttggg tctgtgggtg
     7201 tcaatctcca agcatacaca tattttgtgt ttccaaatgc gtccacatat ttatataagt
     7261 attttccgtc tgttcgttgg ctctctccag tatgcaggat acgtcctttg ctatcccgtc
     7321 ttttttcttt catggtgtct gctcctttcc ttgttggaaa gagccttgat atgacttgtg
     7381 ttcatcataa cacatacaag gctcatttgc attagattgc gtccaattta tcaatgacct
     7441 tttcaaactg tcggcgttta atctgtatgc ggttgccatt cataatgagc cagccagcgt
     7501 ccttgttttc ctctgccaag cgtcttaatt tgttttctcc gatacggaaa tattttgacg
     7561 cttcttcaat ggtaagggtg tatttttccc atacgggaat atcgttgtta ttcatcagat
     7621 accccctttc ccatgtttcg tgtcatactg gatatagggg ataaggtcgg tgcggtttat
     7681 cctctgtaag tgagcggtca gtttactgga aagggaagtg ctgtatcttg ccttatcaag
     7741 catagttccc actttttcca gtccacctaa tgcgtcatgt tcttccaaga aatagaaact
     7801 tttgacagca gaaatcacgc cacccgctgt gagccattgc tgtgtttctt caagggaatt
     7861 atgtggtttg ggtacttgca gtttcaagac ttccacagcc cctaaaaagc gttcccaaaa
     7921 ccgacacgtt ttccacctgc tcttattact ttcatttctg ttcgctacga caaagcgtag
     7981 gttgtttgcc aatagcccga aagccagttc cccaagttcc agtggtctgt ccttgaatgt
     8041 catggcaaaa acatgagcct tatcatcacg cagttgcatt tctgtccgtt tccaactgcc
     8101 gacttcatca agtgtcttat tatattttga acagacttcc ttatccttgt cataaaagcg
     8161 gtaggacaat cccgattttc cagcaccgat ataaaccgtt tttgcggtgt cgaaatcgtc
     8221 aaacttgctt tcatcaaagt ggtagccctc actattggaa ataaattcct ctttttcgca
     8281 tttcttcttt atctgctcta tggtaaagaa tggcttttcg ttcttatcat caatggcaat
     8341 atcaagtctt gtgaagtgga aattatccag tccgtatctt ctttcacaat gtttgaacat
     8401 atccccaaag gtatagtttc tgctgttgag aatacggaaa atatcatcac aacctctgcc
     8461 agtcataaca agataacagc ctaacccttg tgggttgtcc tctgtctttc ttgcgtcccc
     8521 cgatacataa atatctccaa tctcccagcg tatctgatag gtcttgaatt taacggttgc
     8581 cggatagaca ttgaatatgt cagtcggtaa ctctaaaatg tgtgtgatta catcttctgc
     8641 ggttgcggtt tcaaatacaa tgctgatgta atcaatctta acggataaat tttcgtgtgt
     8701 tgcgatactt cattactcct ttcgtatgcc ccgatttttt gcggggtata aattgcattt
     8761 tttcctttat ttatcacagt ttctaggtac tatgacatgt atacgcaggg cggtgttaca
     8821 tatacgccct gtacgaacgc ctaaaggcgt tccctttcca aaaggaactg tctgtccgaa
     8881 acacgtctgg gggacgtgcc ccgtccagcc agcccctttt ggaaacccga ttttcagtct
     8941 gatagtccat gcttgttcat gctatcaaag tgaaaatata tcttttctat gcttactcaa
     9001 gcgtgtgatt ggccctagtg tgcttaatca cttcatccga tacgataata acgccattag
     9061 aattacttgt caagaagttt ttgttgactt tttgatacag attaagtatg atatgcttat
     9121 aaataccaag aaaagcaacg aaaggagagg ttatactaag ttatggagca ttatgaaaag
     9181 aaaatcagtt ttttaggaga aaacatacaa accataagaa aacacagagg aatgaaacag
     9241 caggaacttg cggataaaat cggtatcaat atgcagagcc tttccaagat tgaacgaggt
     9301 gtgaattttc ctacctttga tacgctggaa aagataatag acgtgctggg agtaacaccc
     9361 aatgaattgt tgtcggggga atggaagttt gtcaatcagg gagaaaaaga agtctgccag
     9421 tttttaaaaa tcgaagaacg tctaaatgca gaactaaaac atggacacta tgacaacttc
     9481 tttgatagcg aggaagaatg gctggaatat gagttagaaa agttacggga atatattacc
     9541 gattacatca acgggaaaag cattgtagcg tccgaccttt acccaatcaa agaatttatc
     9601 cagcatttga aattccaaaa actactagac cgctatgatg atttatacag catggatatg
     9661 ttcggagaaa gcatagtggg acataaatat aggacaccac atcaagtagt gaaaatgata
     9721 aacccgaact caaaagagga tatggaactg ttacgggaag tattaaagaa taaccatttt
     9781 gatgatgagg acgaataatc tttttcctct tgtgagaaga tacagaaaag aaaaagccca
     9841 atcaagagtg caagcaccac caatggtggc aagctcttga cagggctttt tcttttctgt
     9901 tgtgggtaat caagaggaag aaagaagtaa aatctgattt gtgacaaatc aggcaatatc
     9961 aacgcaagtg tcgcagtatg agttgtacta cataagaata gtaactggaa gattgtaaga
    10021 taatcaaaaa ggcgtgtatt tcaaattaga gatacacgtc tttgaaatgc acaaagttta
    10081 ttgaatgtta ttcattgaca atcgttcatt cacgagatat aatttttgtg aaaggagttg
    10141 atcacatgaa tattacgaaa caaaagtcac aaaagacctt tgataagcaa gcagccgatt
    10201 atgatacaaa cattcaggga gaacacgctc gaaagctcta taagccgatt atagaaaatt
    10261 taaaaaataa gaatatccat tctatattag atttaggttg tggaacgggt gcgttgctta
    10321 aaaaaataaa agaattaaat atagcagaac aactatttgg aatagatata tctcccaata
    10381 tgcttgaaat tgcaagaaat aaactaggaa acgatgcaac acttatctta ggcgattctg
    10441 agagattacc ttttgaagat aattcttttg acgccattgt ctgtaatgac tcatttcatc
    10501 attatccact accagatatt gtagcgaaag aagtgtctag gtgcttaaag caaaatggag
    10561 tattcattat cggcgattgt tggcaaccaa tcggagcaag acagataatg aattactata
    10621 tgaaacatag caatagtggg gatgtaaaaa tttattcaaa aaaggaaatg ttgttattgt
    10681 tatcaaaaga cttccacgaa attgaatgga aatcttttgg cacaacatct tgtttaatta
    10741 tagcatacaa taactgaacc gaaggaaagg aaagaaaaca atgcgtattt caaaagaacc
    10801 agaggaacgt aaacaagaaa ttttagaaac agctattaaa ttatttagtg taaatgggtt
    10861 tgaaaaaact tcaatatcag acatagcaaa agaaattgga atagcacaag gattatgtta
    10921 tagatatttc ccatcaaaag atgtactttt tcagtccgct atcaatgaat atgcagatat
    10981 tttagtcgcc aatatgacaa aaaatataaa tataaaaaag gatagtttaa aagatatttt
    11041 gcggaagatg atacttttta cagaacaaga agatgataca tattatcccg tttttcataa
    11101 taaccaaaat aaaaatttcc ataatttatt aacattgaat gtatgtgaaa aactttttcc
    11161 tattgtatct gaaattattg aaagagcaaa ttgcacaaat gaaatacaag ttaatgatgt
    11221 tgaaatgtat gccagctttt gcatatatgg acaactagga attttattaa ataccgatat
    11281 atcaactaaa gaaaagagca cacggataaa ggcatttttt agggatttgt ttaggcttta
    11341 aatattcccc tgccaaaagg cagggaatat ttattacaat aaaatgaatg attttcattc
    11401 aaaaggagga attaagatga acaaaaatat tgattttgta aacggaaaca cgaaaaaatg
    11461 tttaattgaa atgactgttc ccatgataat agccatgttt ttaaatatga catataatct
    11521 tgtagacagt ctttggattg gaaatttgtt aggtgagcag gcatacgcag cgttaacaaa
    11581 ttctacacct attattttaa ttttaacttc aattggtatg ggagcaacaa acgggttagc
    11641 cattctatta tcacaggtaa ttggtgcgaa aaatgagcaa aagaaaaaga atattatagc
    11701 tacttctttt tttgtatctg taattctttc agtgcttatt acaattgtat tggagatttt
    11761 tttagaacct attttgatag ttttaaatac accgactgaa atttttgata tggcaaagaa
    11821 ttacttatct atatatgttt tgggatatgt agcagtttat ttgtatttat actttacagc
    11881 cgttttaaga agttttggca atactatgtt tcaggcagtt gcgatgcttg tgtctactct
    11941 attgaacgct attttagatc ctattttcat taacataatt ggatttcacg gagcggcaat
    12001 cgcaacctta atatcacaga ttatttgttt ggcttttatg tgcatttata tttatcgcaa
    12061 aaaattattt aacctttcac tatctgattt tgatacaaca gagattatgc cgtttataaa
    12121 taatgctgtg ccgtctgtta ttcaacaaag tatccctgct attagtacaa catttttaac
    12181 tgctattgtt agtacttata gtatttctgc tattgctggc tacggagttg caggaaaatt
    12241 agaaatgata ttattttatc ctgctatggc tcttaatatg gtgttgactt caatcgtagg
    12301 acaatgtatt ggagcacaac gtatcgacag agcaaagaat tatctgaaat tagcattaaa
    12361 atatggtatt attgtttcaa ttgtattatc agcagttgtt attttcttct ctaaacagtt
    12421 ggcaaaattg tttgtttcca acgaagctgt aacaaatgtt gtcagccatt attttttaat
    12481 tgttagtatc ggttatattc tttacactat tactagttgt tacttaggta ctttaaacgg
    12541 catgggaaaa ccaacaaaaa gtatggtact aatgatattc tattatatta ttattcgtat
    12601 tcctcttgcc tatcttttat cttggctgga aacagattta aatggtgttt ggatagcaat
    12661 tttaattagt cacatagttg cctgcattat aactatttta acaagtaatc aaatcattaa
    12721 gcggttatat gggagatttt atgataggac taaaaaaagc aagatataaa aaacattgaa
    12781 agtaactgct atgagaagga cttgtattat ggaaagcttt aagcatactt ccatacgagc
    12841 aacagaaacc taacagtatt ttaccaacaa ctaaatattt tacaacatta aaagttcgta
    12901 cagacagtat gttttgtgcg gacttttttc ttgttctttt ttccgtattt tccaaagata
    12961 tagacggcat ttccgtttat atagtttcgg cgtatgcgga aagggggtga gattatgaac
    13021 acatcttctt tcgagcatat cgttagaata cagtttaatg ctttgatgat gactgttatt
    13081 aaatgcacag taaaaagcag gaacaaacag tttgcaagac gttctaaacg tgaagtttta
    13141 ttctgtgaat tatcagatat gaaaatgttt gagcaaggag cagaggataa ttattcttgt
    13201 aattatacct cttttgaagt cttgaatttc acaatccaaa tatcaaatga gaaacttgct
    13261 gttgctttat ataagttatc gaataaagag cgtgatgtta ttttattacg ctattttcaa
    13321 agcataagcg accaagaaat cgcagaatta tatcatgtat cacgttctac tgtttatcgc
    13381 agaagaagta acggattgaa gagactaaaa acactattga aagagagaaa ttgaaccaat
    13441 gagaaaagaa tatggctatc cggaattaga agtaatatgt aaagcgtctg ctggaaatga
    13501 agcttcaata aaggaaatct tgaatttcta tgacgcttat atatgtaaat tgtgtttgcg
    13561 tccgttttac cactctgaaa gtggtaaaat cactatgcag attgatgaag aattaaaagg
    13621 tcaaattcat acagaaatga tgaaagcaat cttgaaattt gaaatcagag tgaagtaatc
    13681 atattacatg aatgaaaaaa cgggagatac actaatgtct gtgctatctc ccatgttatt
    13741 ttttagtatc atatcaaggc tcattcaatc gtggtaaatt tgtggtaaaa tgaatggttt
    13801 tctatacttc aaaatgcttg aaagtatagt atttatgctg ataatcgaaa atcagatata
    13861 aaatttatat taaagtttag atatctaaaa atacttttag aatttattat attatgttaa
    13921 ttttttatat cttataagga ttg
//
LOCUS       DBGJFF010000100        13824 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.101, whole genome shotgun sequence.
ACCESSION   DBGJFF010000100 DBGJFF010000000
VERSION     DBGJFF010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13824
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.101"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            122..568
                     /locus_tag="ACOIAF_002832"
     CDS             122..568
                     /locus_tag="ACOIAF_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669835.1"
                     /translation="MLTYLMKRQMHKKDDGMSKPMMITGAVITGVGAYAVYKMVKSHK
                     SNSQMVNTNYYGNSDYDDYKYDEFDYDCDCRDTKQDYEYEELKKKVKEFNSRRINCEN
                     CPHVSQEEMMQYVNSVKDVKEELDLHEDAKKDNIYKEDEYKKYEEE"
     gene            853..1557
                     /locus_tag="ACOIAF_002833"
     CDS             853..1557
                     /locus_tag="ACOIAF_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGZ2669836.1"
                     /translation="MLKLLIVEDDSTISFGIKYALEQEGFSIDISKDLSSGKEMISSN
                     EYSMILLDVTLPDGTGYELCQYIRGFSQVPIIFLTACDEEVNIVMGLDIGGDDYITKP
                     FRIRELISRIKAVLRRKGNTSEENKKILKFGDLSIYTLEARVYKNDKEIFLTSVEYKL
                     LLILIQNKNTVLSRTQILEKLWDVTYDFVNDNTLTVYIKRLREKIGDDLCEPIYIETV
                     RGIGYKWIGSENSVSI"
     gene            1535..2788
                     /locus_tag="ACOIAF_002834"
     CDS             1535..2788
                     /locus_tag="ACOIAF_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HGZ2669837.1"
                     /translation="MKIVFLYNPEVKKFLSKYVTLMFIVIIISIGFSIINVSLTKEMV
                     VRNNQAIIGTLSSKYPNLESQIVDIITQGKSMENIDYGQKILIKYNYDKSIRINSEPI
                     ISKLVLETIKINIILVCIIFILIFVLIVRYFTSIYNDLSDMTKYVYYSSEGKPFDMKN
                     KNQEGQIGLLKTELLKMTTILNEKVELLKREKIFLNNTISDISHQLKTPMTSLIMLND
                     LLYNDVPYEVKIDFLNKIKNQLNRMDWLIKSMLKLSKVEAKVINFKKDKVKFSELIHR
                     AMLSMKIPMEIKNQKLTIEGNDNVSYIGDIDWSVEALVNIIKNCVEHTPEFGNIIITY
                     KENPLFSELIIKDEGEGIDKKDIPHVFKRFYRGRSSSKEDSVGIGLAMSKSIIESQNG
                     DIYVNSEKGKGTEFHIIFHKMYDSD"
     gene            2868..3545
                     /locus_tag="ACOIAF_002835"
     CDS             2868..3545
                     /locus_tag="ACOIAF_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2669838.1"
                     /translation="MEILRVENLTKSYGKNETKVDAIKNVSLSVEKGTFIAITGPSGS
                     GKSTLLHLLGGVDRPTSGKVYINDVDIYNLKTKDLAIFRRRNIGLIYQFYNLIPVLNV
                     KENILLPAELDNRDIDREYFDDLMKTLGLIDREKHLPNQLSGGQQQRTSIGRALINRP
                     SIILADEPTGNLDSKNSKEILELLKLSVKKYNQTLIMITHDKNMALQADRIISIEDGI
                     IKSDEVM"
     gene            3546..6068
                     /locus_tag="ACOIAF_002836"
     CDS             3546..6068
                     /locus_tag="ACOIAF_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2669839.1"
                     /translation="MNLYTSLTIRYLKQNKRRTIVTIIGIILSTALICGIGNIFESFM
                     DYQMRETIKNDGSFHVTFYDVNRKNVEYVTKSAEIEKHAFTKQLGYSKLENSENAILS
                     IKQYDKNAINGYKVSIKEGRFPAKEGEIVLSESIINLIDDKLKIGDKITLKVGDMFDS
                     SKKKIDSMTFHEGDYIANEKDRTFKVVGIIEKPGFERYNGIATAIAYFNPMNNYNDTI
                     NVSVAVKNPKDVYKISNKISKNIYSNKDKEAVSDMIKYNEHLLRLQGASKYSNINSSI
                     KSIIIAVTILVIVCTIATVYNSFSISINERKKQFGILNSIGATSSQIKRLVFIEGIII
                     SLIGIPIGLISGTVAIDLLFKIINKYFTESVVTKMSLQIVYNPIIIIVSIIIVLLTIF
                     ISILLPAISASNISPLNVIKNTGEYKVGKIKSSRLIKMIFKTEGVLAYKNIRRNRKKF
                     IITLFSLMVSIIIFISFSGFTLLLLRGEEIRNSQRKYDLYLTAKNTARSVEDTISELE
                     DIDGIKNFELATGQYVSIRVSENKINKSKEDLIRKYYQKYKIGDSYEYDFSNNELIFP
                     GDFAVKNKINNLVQGSFNKERAIEENGKKGVVELTNYKVGDTVNCEYLDENALSKKVK
                     VKILAITDEERLGLGYQNMGLQFITYDEVAKNLNLKLSKSLIFIDSGGSIKTKKEIEA
                     LANKNNFNFYDESASNESEKQDLKVIKIFVYGFIVVISLVSVTNILNTVSTSINLRKR
                     ELAVIQSIGVTPKGFRKMIYLESFIYGILSLLFGIPISIGIILIMNKLISGVIEFSPI
                     IPWTAIVICIVSVFIITFIAGYIPMSKLNKENIIDNIRRESI"
     gene            6289..7563
                     /locus_tag="ACOIAF_002837"
     CDS             6289..7563
                     /locus_tag="ACOIAF_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster-binding protein"
                     /protein_id="HGZ2669840.1"
                     /translation="MITISHITAKNMYKSLEERINKFPQGAPPSDTLYKILNVLYTEQ
                     EAKLVAQLPIKPFRVKTAAKIWSVSESEAYRVLDKLASKALILDIEDNKGKKYIMPPP
                     MAGFFEFAMMRTRHDIDQKLLAELYYQYMNVEEDFIKDLFYSTETKLGRVYVQEEVLT
                     NDNEVSILDYERATHIIDESTHIGISMCYCRHRMQHVGKACDAPMDICMTFDNVANSL
                     INNKFARRVDKIECKELLHQAYEHNLVQCGENVRKGVTFICNCCGCCCEAMVAAKRFG
                     NLHPVQTTSFIPNINHENCVKCGKCITACPIDAISKVKEDGKEYIKIDEDRCLGCGVC
                     VRNCHKNSIMLLKRDEKIITPANSVHRAVLMAIEKGQLQNLIFDNNALASHRAMGAIL
                     SAILKLEPAKKILASKQLKSVYLDKLLSMNDK"
     gene            complement(7724..10765)
                     /locus_tag="ACOIAF_002838"
     CDS             complement(7724..10765)
                     /locus_tag="ACOIAF_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp20"
                     /protein_id="HGZ2669841.1"
                     /translation="MFKQRLSKLLSSTLVLSMLFTAAPNITFADNTKDNSEKYQSSDI
                     ELHDYSKNAESYTKTKALAKEKIQTLLSKYGAVSAQYALIDNGKIEISGNGGVYSKQD
                     NKNLNKDNMYSIASISKMFTTTAVMKLVDDGKLNLDTPVVKYIPEFKMADDRYKEITP
                     RMLLNHSSGLMGSSFKNTILLADNDSYGHDNFLKELQKQRLKAKPGAFSVYCNDGFTL
                     AEILVERVSGMSFTNFLDKYINNPLNLQNTKTPENSFDSSKLAKAYVPYWEDAVPQDN
                     LNAIGAGGLYSSAENLCTFAQTFMKNSNGILSPASVKAMENKEYLNGLWPEGEDSILG
                     YGLGWDCVNTYPFNQYNLKALTKGGDSLLFHSNLIVLPDENMAVAVLSSGGSSQLNEI
                     IGQEILLSALKEKGKIKEIKPDKTFSKPQQVKMPSSLKENSGLYASSNMIKVDVNDNG
                     TLTVSSPYIENGPEDKYVYIGQDRFVSEKGNSCLKFVKEKNNITYLNMSSYDDVPGLG
                     QTASLYYVAQKIDDNNISNSVKEAWKKRNGKDYYLVDEKYTSQSYMFGSVKATLALSD
                     ETPGYIVNTKIMDENNSNAFIEIPGVIGRDLSDIKLHKENGTEYLSFGTLTYVSEDSI
                     TNLPAEKSFTCELESNGYAKWYKIGDDIANKKIEVNLPQNSSFAVYDDKGVPVNYSLV
                     TKNNRVRLPKGGVIVFLGSPNARFEVTYQDEVNASALTGTDRYETSIKISQAGWENAE
                     NAVLINDSAIADALAATPFAYKKNAPILLTGSSQINEKTLAELKRLKVKNVYVVGGEA
                     SINEKSLDTIKSNNISVSRISGSDRYQTSMNIAKELNNISNISKISVVNGEKGLADAV
                     SIGAVSAQNDMPIILTNENSNITEINNLFKNKKIDKSYVIGGEYTVSKNIESKLQNPQ
                     RISGSTRNETNAKVIKEFYKDSKIDNLYVAKNGMNKQDDLIDGLSVGVLAGKTKSPVM
                     LVGNSLDYNQKELFKTMRFKSVTQIGGNGNENSFKQIKEIA"
     gene            10971..11270
                     /locus_tag="ACOIAF_002839"
     CDS             10971..11270
                     /locus_tag="ACOIAF_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HGZ2669842.1"
                     /translation="MYKNINKKQLKEMMKNEKDVLLLDVRTKDEFKEYKIENAINISL
                     QELINNIDEIYDYKDKKVVVYCRSGHRSVTACNLLAEEGFEHLYNLNSGIIDYMS"
     gene            complement(11508..13736)
                     /locus_tag="ACOIAF_002840"
     CDS             complement(11508..13736)
                     /locus_tag="ACOIAF_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HGZ2669843.1"
                     /translation="MRHLKKTNSLKEILNWNNIKNLRPSKGLKNILEIFKSDLKDIRG
                     NYAVLVIVLGLAFLPSLYAWFNIKASWDPYGSTANMLVAVTNEDKGNEIAGQKINVGD
                     QLIEQLKDNKSLGWQFVDEKTALEGVKKGTYYASLKIPKNFTKDLTSLITDDVKKGEI
                     IYTVNEKINAVAPKITDKGASTIQLQVNQTIVKTVSEIVLGISNGVGAGIEHSLPKLT
                     KVENSLIDLQKRFDHINKTVDLASDATSKLSDTVKDIKSDLPTIKKTLNDTKLLSSDL
                     KKFLEDTNDNLDELSPLIKSDLNLMVDLSSSASSLTLNLIDAVNSGSEDVPKLIDNLS
                     EKLSNLQSLNDTLVDFLTKLNQLTSNNRLDDVIDNLEDSSNKIDSSISTLNDIKNKVI
                     SGQQPSISALNNVLSLSNGIGRINLNILNNFDSKISKPINNIFANSIKVANDIITVLD
                     KAEAKLPKVEEILTTSLKLSGNAQESISLIRERLPLAKGMLDDLIDTLSKISNGEDMK
                     KLVSLLESDILTKSNFLKEPVEVKTEKLYPIENYGSAMTPFYSVLSTWVGILLISALL
                     STSVHGNYKPYEIYFGRGLTFLSIALVQGFIIGTGDILLLGVTAKHPILLIVLLMFTS
                     MVFNFIVYSLVSVFGNVGKAIAIVLLVVQIGSSGGTFPIQVTPTFFQRINPIMPFTYA
                     IGATREAVGGIYAPNLTKDILVLLLFMILSICLNVLLKGPINRVIKPFNDKFGDSNLT
                     GH"
BASE COUNT         5261 a         1650 c         2102 g         4811 t
ORIGIN      
        1 caaacacaaa atataagttt atattttgta aaaattgtaa tttaaaaaat aacataattt
       61 ttattaaaat aatacagata ctatttatag atagaaataa aaattagaaa ggagaaaaaa
      121 tatgttaaca tatttaatga aaagacaaat gcataaaaaa gatgatggaa tgagtaaacc
      181 aatgatgatt acaggagctg taattacagg tgtaggagca tatgctgtat ataaaatggt
      241 aaaaagccat aaatctaatt ctcaaatggt caatacaaat tattatggaa atagcgatta
      301 tgatgattat aaatatgacg aatttgatta tgactgtgat tgtagagaca caaaacaaga
      361 ttatgaatat gaagaattaa aaaaaaaagt aaaagaattt aattcaagaa gaataaattg
      421 tgaaaattgt ccacatgttt ctcaagaaga aatgatgcaa tatgtaaata gtgttaaaga
      481 tgtaaaggaa gagcttgatt tacatgaaga tgctaaaaaa gataatattt acaaagaaga
      541 tgaatacaaa aaatatgaag aagaataagt aatattttgt ataactgaac aaatagtaat
      601 gtcaaattat ttaatatcta gttttaatat gtaagttgta acttaacttg aaagaatcag
      661 tatcattctt tagatagtta agtaacaact tacattttat ttttataaat tttaatagct
      721 ctttgtgact attttgtaat tttagaaata ttttagtatc cagggtaatt gtgtataata
      781 aaacttgtat agatagatag tatgaaatta agcagatagt ttaaaattaa aatgaaaaga
      841 aaggaaatag ttatgttaaa gttgttgata gttgaagatg atagtactat atcttttgga
      901 ataaagtatg ctttagaaca agaaggattt agtatagata tatcaaagga tttatctagt
      961 ggaaaagaaa tgatttcttc taatgaatat agcatgatac tgttggatgt tacattgcca
     1021 gatggaactg gttatgagct ttgtcaatat ataagaggat tctctcaggt tcctataata
     1081 ttcttgactg cttgtgatga agaagtaaat attgtaatgg gtctcgatat aggtggagat
     1141 gattatataa caaaaccttt tagaattaga gaattaatat ccagaataaa agctgtttta
     1201 agaagaaaag gtaatacttc agaagaaaat aagaaaatac ttaaatttgg agatttaagt
     1261 atatatacat tagaagctag agtatataaa aatgataaag aaatatttct gacatcagtt
     1321 gaatataaac ttcttttaat tcttatacag aataaaaata ctgttttaag tagaactcaa
     1381 atattggaaa aactttggga tgtaacttat gattttgtaa acgacaatac tctaactgtt
     1441 tatataaaaa ggcttagaga aaaaataggt gatgatttat gtgaaccaat ctacatagaa
     1501 actgtaaggg ggattggtta taaatggata ggaagtgaaa atagtgtttc tatataatcc
     1561 agaagtgaag aagtttttaa gtaagtatgt tactttgatg tttattgtta tcataatatc
     1621 tattggattt agcataatca acgtgtcctt aactaaagag atggttgtaa gaaataatca
     1681 ggctataata ggaactttat caagcaagta tccaaactta gaaagtcaga tagtagacat
     1741 aataactcaa gggaagtcta tggagaacat agattatggt cagaaaatat taattaagta
     1801 taattatgat aaatctatta ggataaactc tgagcctatt atttctaaat tagttttaga
     1861 gactatcaaa ataaatataa tattagtatg tataatattt attttgatat ttgtgttgat
     1921 tgttagatat tttacgtcaa tttacaatga tttatcagat atgactaagt atgtttacta
     1981 tagttcagag ggtaagccat ttgatatgaa aaacaagaat caagaagggc agataggtct
     2041 tctaaaaact gaactcttaa aaatgacgac aattcttaat gaaaaggttg aacttttaaa
     2101 aagagaaaag atatttttaa acaatacaat ttccgatata tcacatcaat taaaaactcc
     2161 tatgacttct ttaataatgt tgaatgattt attatataat gatgtaccat acgaagtaaa
     2221 aatcgatttt ttaaataaga taaaaaatca attaaataga atggattggt tgattaaaag
     2281 catgcttaaa ttatcaaagg tagaagcaaa agtgataaat tttaaaaaag ataaggtaaa
     2341 atttagtgaa ctcatacata gagcaatgct atctatgaaa atacctatgg agattaaaaa
     2401 tcaaaaactg actatagagg gaaatgataa tgtatcttat attggtgata ttgattggtc
     2461 cgtggaagca ttagtgaata taatcaaaaa ttgtgtagaa catactcctg aatttggaaa
     2521 tataattata acctataaag aaaatccatt attttctgaa ctaataataa aagatgaggg
     2581 tgaaggtata gataaaaaag atataccaca tgtatttaaa cgattctata gaggaagaag
     2641 tagttcaaaa gaagatagtg taggaatagg tcttgcaatg tcaaaatcta taatagaaag
     2701 ccaaaatggc gatatatatg taaacagcga aaaaggtaag ggtacagaat ttcatataat
     2761 atttcataaa atgtatgata gtgactaatc tgtaatattt aattcacttt atagtaatag
     2821 ccaaaaccta atatatgaag tatagaatat aaatattgga ggtagttatg gaaattttaa
     2881 gagtagaaaa tctaactaaa agttatggta aaaatgaaac aaaagttgat gctataaaaa
     2941 atgtaagttt atcagttgaa aaaggaacgt ttatagcaat aacaggtcca agtggaagtg
     3001 gtaaaagtac cttattacat ttattaggag gagttgacag accaactagt ggtaaggttt
     3061 atataaatga tgttgatata tataatttaa aaactaagga tttggcgata tttagaagaa
     3121 gaaatatagg tcttatatat caattttaca acttaattcc agtcttaaat gtgaaggaaa
     3181 acatattgct tccagcagag cttgacaata gagatattga tagagaatat tttgatgatt
     3241 taatgaaaac attaggtcta attgatagag aaaagcacct tccaaaccaa ttatcaggag
     3301 gtcaacaaca gagaacctct ataggacgtg cattaattaa tagaccttct ataatattag
     3361 cagatgagcc aacaggtaat ttagatagta aaaattcaaa ggaaatatta gaattgttaa
     3421 aattgtcagt aaaaaaatac aaccagacac taataatgat aactcatgac aaaaatatgg
     3481 cacttcaagc tgatagaatt ataagcatag aagatggaat cataaaaagt gatgaggtga
     3541 tgtagatgaa tttatataca tcactaacta ttagatactt aaaacaaaat aaaagaagaa
     3601 ccattgtaac cataatcggt ataatacttt caacagcttt aatttgtggt ataggaaata
     3661 tatttgaaag ttttatggat tatcaaatga gagaaactat aaagaatgat ggaagtttcc
     3721 atgttacatt ttatgatgta aatagaaaaa atgtggaata tgtaacgaaa tcagctgaaa
     3781 tagaaaaaca tgcatttacc aaacaacttg gatattctaa attagaaaat agtgaaaatg
     3841 ctatattaag tataaagcaa tatgataaaa atgcaataaa tggatataaa gtttctataa
     3901 aagaaggtag atttccagct aaggaaggag aaattgtttt aagtgaaagt attattaatc
     3961 ttatagatga taagttaaaa ataggagata agataacttt aaaagtaggg gatatgtttg
     4021 actcaagcaa gaaaaagatt gatagtatga catttcatga aggtgattat atagcaaatg
     4081 aaaaagatag aacttttaaa gttgttggta taattgaaaa acctgggttt gaacgttata
     4141 atggaattgc aacagctata gcctacttta atcctatgaa taattacaat gatacaatta
     4201 atgtatcagt agcagtaaaa aatcctaaag atgtgtataa gataagtaat aaaataagta
     4261 aaaatatata ttcaaataaa gataaagaag cagtttctga tatgataaaa tacaatgaac
     4321 atttattgag gttacaaggt gctagtaagt attcaaatat aaatagctct ataaagtcta
     4381 taattatagc agtaactatt ttagtaatcg tatgtacaat tgctacagtg tacaattcct
     4441 ttagtatttc tataaatgaa cgaaaaaaac agtttgggat attaaattct ataggtgcta
     4501 catctagtca aattaaaaga ttagttttta tagaaggtat tataattagt ttaattggta
     4561 taccaatagg actaatatct ggaacagtag ctatagactt attgtttaaa attataaata
     4621 agtattttac tgaatcggtt gttactaaga tgagtttgca gatagtgtat aatccaataa
     4681 taattattgt tagtataata atagttttgc ttacaatctt tatatcaatt ctactacctg
     4741 caatatcggc ttctaatatt tctcccctta atgtgattaa aaatactgga gagtataaag
     4801 tgggcaaaat caaaagctca agacttatta aaatgatttt taaaactgaa ggagtacttg
     4861 catataaaaa tattaggaga aatagaaaaa aatttataat aactcttttt tctttaatgg
     4921 taagtataat aatatttata tcttttagtg gctttacatt acttttacta agaggtgaag
     4981 aaattagaaa ttctcaaaga aaatatgatt tgtatttaac tgcaaagaac acagctaggt
     5041 ctgtagagga tactataagt gaactagaag atattgatgg gataaaaaac tttgaattgg
     5101 caacaggaca atatgtgtct ataagagtaa gtgaaaataa aataaataaa tccaaggaag
     5161 atttaatcag gaaatattat caaaaatata agattggaga tagttatgaa tatgatttca
     5221 gtaataatga attaattttt ccaggagatt ttgcagtaaa aaacaaaata aataacttag
     5281 tacagggctc ttttaataaa gagagagcaa ttgaagaaaa tgggaagaaa ggtgtagtag
     5341 aacttaccaa ctataaagta ggagatacag taaattgtga atatcttgat gaaaatgcct
     5401 taagtaaaaa agtaaaggtc aagattcttg ctataactga tgaagaacgt ctaggtcttg
     5461 gttatcaaaa tatggggtta caatttataa cttatgatga agttgctaaa aatttaaatt
     5521 taaaattaag taaaagcctt atatttattg attcaggagg aagcattaag actaagaagg
     5581 aaatagaagc tttggcaaat aaaaataatt ttaactttta tgatgaaagt gcaagcaatg
     5641 aaagtgaaaa acaagattta aaggttatta aaatatttgt atatggtttt atagtagtga
     5701 tatctttagt gagtgttaca aatattctaa atacagtaag tacaagtata aatctaagaa
     5761 aaagagaact tgcagtaata caatctatag gggttactcc taaaggattt aggaaaatga
     5821 tatatttaga aagttttata tatggtatac tgtcattgtt gtttggaata ccaattagta
     5881 ttggtataat tctaattatg aataaactta tttcaggtgt aattgaattt tctccgatta
     5941 taccgtggac agccatagtt atctgtatag taagtgtttt tataataact tttatagcag
     6001 ggtatatacc aatgagtaaa ttaaataaag aaaatataat agataatata agaagagaaa
     6061 gtatatagat ataaaaataa ttcttaacta tataaaatat tattaaaatc atttaagtgg
     6121 tataaaatat tattatatca cttaaatgat tttttattta tataagaaat ctaataataa
     6181 attactagag gtatttaact atttagaaag aattggtatt atataaagta agagaatata
     6241 taataagaaa gaatatttga ttttattgga aaacaaaaga aaaagaaggt gattactatt
     6301 tctcatataa ctgcgaagaa tatgtataaa agtttagagg aaagaataaa caagtttcca
     6361 caaggtgctc caccatctga tacattatac aaaatactaa atgtactata tacagaacaa
     6421 gaggcgaagt tagttgctca gcttccaata aagcctttca gagtaaaaac agcagctaaa
     6481 atttggagtg taagtgaaag tgaagcatat agagtattgg ataaacttgc tagtaaggct
     6541 ctgatactag atatagaaga taataaaggg aagaaatata taatgcctcc tccaatggct
     6601 ggtttttttg agtttgccat gatgagaacg agacacgata tagaccaaaa acttttggca
     6661 gagctttact atcaatatat gaatgtagaa gaagatttta tcaaagattt attttattca
     6721 acagaaacga agcttggaag agtatacgtt caagaggaag ttttaactaa tgataatgag
     6781 gttagtattt tagattatga aagggctact catataattg atgaatcaac tcatataggt
     6841 ataagtatgt gttactgtag acatagaatg caacatgttg gaaaagcttg tgatgctcct
     6901 atggatatat gtatgacgtt tgacaatgta gcaaattctt taataaataa taaatttgca
     6961 agacgtgtgg ataaaataga atgtaaagaa ctacttcatc aagcatatga acataactta
     7021 gttcaatgtg gagaaaatgt taggaaaggg gttacgttta tatgtaattg ctgtggatgt
     7081 tgctgtgaag caatggtagc agctaaaaga tttggaaatc ttcatccagt acagacaaca
     7141 agttttatac caaatataaa ccatgaaaat tgtgtaaagt gtggtaaatg tataactgca
     7201 tgtccaatag atgcgataag caaggttaag gaagatggta aagaatatat aaaaattgat
     7261 gaggatagat gtttaggttg tggagtttgt gttagaaatt gccataaaaa tagcattatg
     7321 ttattaaaga gagatgaaaa aataataact ccagcaaatt cagtacatag agcagttctt
     7381 atggcaattg aaaaagggca gttacaaaat ttaatttttg ataataatgc tctggctagt
     7441 catagagcta tgggagctat actgtcagct atattaaagc ttgaaccagc taaaaagatt
     7501 ttagccagta aacaattaaa gtcagtatat ttagataaat tgttaagtat gaatgataaa
     7561 tagaaactaa cttaaaaaag gcaaagtttt agacttatct atatcttaat aaacttatca
     7621 tttaatattt aaatttaatt tttacacata taaaacttat aacttaaata aaaaaaggct
     7681 atattcaatt aatattactt gaatacagcc ttttttagtt atgttaagct atttctttta
     7741 tttgcttaaa tgagttttca tttccatttc caccaatttg tgttacagat ttaaatctca
     7801 tagttttaaa tagttctttt tgattataat ctagtgagtt tcctactagc attacaggag
     7861 atttagtttt tccagccaaa actccaacag ataatccgtc tattaaatca tcttgtttat
     7921 tcattccatt tttagcaaca tataaattgt ctatcttaga atccttgtaa aattctttta
     7981 ttacttttgc attagtttca tttctagtgc tacctgatat tctctgtgga ttttgaagtt
     8041 tactttctat atttttagaa actgtatatt ctccaccgat tacataagat ttatcaatct
     8101 ttttgttttt aaacaagtta tttatttcag ttatattact attttcatta gtaagtatta
     8161 taggcatatc attttgagct gatacagctc caatgcttac tgcatcggct aatccttttt
     8221 cgccattaac tactgaaatc tttgatatat tgctaatatt gttcaattct ttagcaatgt
     8281 tcatagaagt ttgatatcta tcacttcctg aaattctaga tacagagata ttgtttgatt
     8341 ttatagtatc taaagatttt tcatttatag aagcctctcc accaactaca tatacatttt
     8401 taacttttaa tctctttaat tcagccaaag ttttttcatt tatttgagag cttccagtca
     8461 ataatatagg agcgtttttc ttgtatgcaa atggtgttgc agccaaagca tctgcaattg
     8521 ctgaatcgtt tattaaaact gcattttcag cattttccca acctgcttga cttatcttaa
     8581 tagaagtttc atatctatca gttcctgtaa gtgcacttgc atttacttca tcttgatatg
     8641 taacctcaaa tctagcattt ggacttccta agaaaacaat tacgcctcct tttggcaatc
     8701 taactctatt gttctttgta actaaagaat agtttacagg aacaccttta tcatcataaa
     8761 cagcaaaaga tgaattttga ggaagattaa cttctatttt tttatttgcg atatcgtcac
     8821 caatcttata ccattttgca taaccattgg attctagttc acaagtaaaa gatttttctg
     8881 ctggcaaatt agttatagaa tcttcactaa catacgttag tgttccaaaa gataaatact
     8941 ctgtaccatt ttctttatga agtttaatgt cggataaatc acgacctatt acaccaggta
     9001 tttctataaa agcatttgag ttattctcat ccattatttt agtgttaact atataacctg
     9061 gagtttcatc agaaagagca agagttgcct tcacagaacc aaacatatag ctttgagaag
     9121 tatatttttc atctactaga tagtaatctt taccatttct ttttttccat gcttctttta
     9181 cactatttga tatattgttg tcatctatct tttgagctac atagtataaa cttgctgtct
     9241 gacctaatcc agggacatca tcataagagc tcatatttaa atatgtgata ttgttttttt
     9301 cttttacaaa ttttagacag ctgttacctt tttcagatac aaatctatct tgacctatgt
     9361 atacgtattt atcttcaggt ccattttcta tataaggaga agatactgtt aaagtaccat
     9421 tatcattaac atctactttt atcatatttg aagaagcata aagaccacta ttttctttta
     9481 gagagctagg cattttaact tgttgtggtt tactaaatgt tttatcaggt tttatttctt
     9541 taattttacc tttttctttt aatgcagata gtaagatttc ttgtccaatt atttcgttaa
     9601 gttgactact tccaccagaa gaaagaactg caactgccat attttcatct ggaagaacta
     9661 taagattact gtggaataat aaagagtctc caccttttgt aagagctttt aaattatact
     9721 gattaaatgg ataagtgttg acacaatccc aacctaaacc atatccaagt atagaatctt
     9781 ctccttcagg ccatagacca tttaaatatt ctttgttttc catagctttt actgaagcag
     9841 gagaaagaat accatttgaa tttttcataa atgtttgtgc aaatgtacat agattttctg
     9901 cacttgaata taaaccacct gcacctatag cattaaggtt atcttgcgga actgcatctt
     9961 cccaataagg tacgtatgct tttgctagtt ttgaactatc aaaactattt tctggagttt
    10021 ttgtattttg taagttaagt ggattattta tgtatttatc aagaaaatta gtaaatgaca
    10081 taccagatac tctttctact aaaatttcag ctaaagtaaa tccatcatta caataaacag
    10141 aaaaagctcc tggtttagct tttaatcttt gtttttgtaa ttcttttaag aaattatcat
    10201 gaccataaga atcattgtct gctaataaaa tagtgttttt aaaactactt cccatcaacc
    10261 ctgatgaatg atttaataac attcttggag tgatttcctt gtatctatca tcagccattt
    10321 taaattcagg tatatacttt acaacaggag tatctaaatt taattttcca tcatcaacca
    10381 acttcataac agctgttgta gtaaacattt tactgataga tgcgatacta tacatattat
    10441 ccttatttaa gtttttgtta tcctgtttgc tgtaaactcc accattacca gaaatctcaa
    10501 ttttgccatt atcaatcaaa gcatattgag cacttacagc accatacttt gatagcaaag
    10561 tttgaatttt ctctttagca agagctttag tttttgtgta agattccgcg tttttactat
    10621 aatcatgtaa ttctatatca gaactttgat atttttcgga attatctttt gtattatcag
    10681 caaaagtaat atttggagca gcagtaaata acatactcaa tactaatgta ctagataata
    10741 atttagagag cctttgctta aacatattac attacctctt tcccttttta ataatttata
    10801 attagcataa cattcttaat tattttaatc aacagattat tatcaataaa aaaagctttt
    10861 ataagtatag ctttttatat aacagagaaa aatataaaaa taatatttgt tgttgtataa
    10921 agaataaagg gtataggtaa tatataaata aaatggagga attttagaca atgtataaga
    10981 atataaataa aaagcaactt aaagaaatga tgaaaaatga aaaggatgtt ttattattag
    11041 atgtaagaac taaagatgaa tttaaggaat ataaaataga aaatgctata aatatatctt
    11101 tacaagagtt aatcaataat atagatgaaa tttacgatta taaagataag aaagtagtag
    11161 tttattgcag aagtggtcat agaagtgtta ctgcatgtaa tttgttagct gaagaaggtt
    11221 ttgagcattt atataattta aattcaggaa taattgatta tatgagttaa ataatgtaag
    11281 ttatacatta aatactatgt atagctctgc ttattaagta ggaatatatc taaatattta
    11341 taagataaat ttaggaaaga acgaattttt tcgttttttt ctatgacagt ttttgtagta
    11401 aatattagtt tttaagttgg aaaaaggtag tagcttttta gctactacct tttaatatga
    11461 acttagaata ttattaaaaa aatatattta aagacagaat taatttttta atgaccagtt
    11521 aaattactat cgccgaattt atcattaaat ggttttataa ctctatttat aggtcctttt
    11581 agaagtacat ttaaacaaat agacagaatc ataaatagta atagaactag tatatcttta
    11641 gttagatttg gagcatatat accaccaact gcttcccttg tagcacctat tgcataggta
    11701 aatggcatta tagggtttat tctttggaag aatgtaggtg taacttgtat agggaatgta
    11761 ccaccagaag aaccaatctg aacgactaat agtactattg caattgcttt tcctacattt
    11821 ccaaatacag aaactaaaga atacactata aagttaaata ccatactagt aaacataagc
    11881 agaactatta ataaaattgg atgttttgct gttactccta acaataatat atctccagtt
    11941 cctattataa aaccttggac aagagctata cttaaaaaag taaggcctct tccaaagtaa
    12001 atttcataag gtttatagtt tccatgtaca gatgtagaca atagtgctga aataagtaat
    12061 ataccaaccc atgtagaaag aacactataa aatggtgtca ttgcagaacc ataattttca
    12121 attggatata atttctctgt tttcacctca acaggttctt ttaagaaatt agactttgtc
    12181 aatatatcac tttcaagcaa gctgactaat tttttcatat cttcaccatt actaattttg
    12241 cttaaagtat caatcaaatc atccaacatt ccttttgcta aaggaagtct ttctcttatc
    12301 aatgatatac tttcctgtgc atttcctgaa agttttagag aagtagtaag tatttcttca
    12361 acctttggaa gttttgcttc tgccttatct aaaacagtaa ttatatcatt tgctactttt
    12421 atactgtttg caaatatatt atttataggt tttgatattt ttgagtcaaa attatttaaa
    12481 atatttaaat taattcttcc aataccattt gatagactta aaacattatt caaagcactt
    12541 atagatggtt gttgtccaga tataactttg tttttaatat catttaatgt tgatatagat
    12601 gaatctattt tattgctaga gtcttcaaga ttatctatta catcatccaa tctattattt
    12661 gatgtaagtt ggtttaattt agttaaaaag tccaccaaag tatcgtttaa agattgcaaa
    12721 ttagaaagtt tttctgataa attatctatc aacttaggta catcttcact gccactattt
    12781 actgcatcaa ttaaatttaa tgtaagagaa gaagcagaag atgaaaggtc aaccattaaa
    12841 tttaagtcag acttaataag aggagaaagt tcgtctaaat tatcatttgt atcctctaag
    12901 aattttttta agtcacttga taatagtttt gtatcattca gagttttttt aattgtaggt
    12961 aaatcagatt taatatcttt aactgtatca gataattttg aagtagcatc agaagctaaa
    13021 tcaacagtct tatttatatg gtcaaatctc ttttgaaggt caataaggga attttcaact
    13081 tttgtcaatt ttggaagact gtgttcaatt cctgcaccaa ctccgtttga tataccaaga
    13141 acgatttcac taactgtttt tactatagtc tgattaacct gtagttgtat tgtagaagca
    13201 cctttatctg taatctttgg tgcaactgca tttatttttt cattaacagt atagataatt
    13261 tcgccttttt taacatcatc tgtaattaaa gatgtcaaat cttttgtaaa attttttgga
    13321 atcttaagtg atgcataata agtacctttt ttaacacctt caagagcagt tttttcatct
    13381 acaaactgcc aacctaaact cttgttatct ttgagttgtt caattaattg gtctcctaca
    13441 ttaatctttt gtcctgctat ttcatttcct ttatcttcat ttgttactgc tacaagcata
    13501 ttagcagtgc ttccataagg gtcccaagat gcctttatat tgaaccaagc atacagagat
    13561 ggtaaaaaag caagacctaa aactattaca agtacggcat aattgccacg tatatctttt
    13621 aggtcacttt taaaaatttc taatatattt tttaaaccct ttgaaggtct taaattcttg
    13681 atattattcc agtttaaaat ttcttttaaa ctattagttt ttttcaaatg tctcacctcc
    13741 taaaatattt tgataaagaa aaaagttaga tttataatca attttaaatt atattacttt
    13801 ttacacaaat agtaaagtta aaat
//
LOCUS       DBGJFF010000101        13735 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.102, whole genome shotgun sequence.
ACCESSION   DBGJFF010000101 DBGJFF010000000
VERSION     DBGJFF010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13735
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.102"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(84..842)
                     /locus_tag="ACOIAF_002841"
     CDS             complement(84..842)
                     /locus_tag="ACOIAF_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HGZ2669844.1"
                     /translation="MREYIAFTKKEFKENLRNYKLFSLIILFLIFGISSPISAKFMPD
                     LIAHFAPTLKVTAAPTALDSWTQFFSNISGLGMSLTLIIFCNILSNEYSKGTLVIMLT
                     KGLSRSSIVLSKFSVTVIIMTIGFWLSFLFAYGYTIYFWATANLNHIIFSAFNLWLIG
                     IMYISVLILGCVLFRPAFASVLLVLVATLLLSLISIPKQIAPYTPNFLMSKNIDLISG
                     KVAMPEFIIPIIITVLISVVCLLAAVILFNKKPV"
     gene            complement(839..1759)
                     /locus_tag="ACOIAF_002842"
     CDS             complement(839..1759)
                     /locus_tag="ACOIAF_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGZ2669845.1"
                     /translation="MMNIVSINGLSKGFGNRKIIDNLNFTVPEGSVFGFVGKNGAGKT
                     TTMKMVLGLLKPDSGTIDVCGEKVTYGKTSSNRHVGYLPDVPEFYNYMRPLEYLSLCG
                     EITGLSKKEIQIRSEELLSLVGLGNEKRRIGGFSRGMKQRLGIAQALLSRPKLLICDE
                     PTSALDPVGRKEILDIMLKIKDSTTVIFSTHILSDVERICDHVAILNKGSIALSGTLS
                     EIKSMHGKDRLLLEFASNDEIQKFKSSDGIKSLLKDSEETNMEIVLHGKDIKAIQKTV
                     ISTLAEMNLCPVKMELIELSLENLFLEVVK"
     gene            complement(1756..1956)
                     /locus_tag="ACOIAF_002843"
     CDS             complement(1756..1956)
                     /locus_tag="ACOIAF_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLD nuclease N-terminal domain-containing
                     protein"
                     /protein_id="HGZ2669846.1"
                     /translation="MENLMEYLPLLVPVIILDLILIITALVHVLRHPNYKIGNKAIWI
                     IVVLFISLIGPILYFTIGRGEE"
     gene            complement(1961..3055)
                     /locus_tag="ACOIAF_002844"
     CDS             complement(1961..3055)
                     /locus_tag="ACOIAF_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGZ2669847.1"
                     /translation="MNELNIAKTLILKRKEKGITQDELANYIGVSKASVSKWETGQSY
                     PDITFLPQLATYFNITVDELICYEPQMMKEDINKLYNKLCKDFTVKPFDEVMDEIREI
                     IKKYYSCFPLIFRMGLLIVNHYDIVDEKKRELLISEALEIFIRIQETCNDIDICRQAK
                     SMEATCYILLNQPIQVIDLLQNSNFPIINESILLAQGQMMNGQMDEARETFQLGAYQN
                     LISLVQNLVGILQNADKLQMKEIERRILAISDIFELDTLSPAIMLSSYLTQAQINLIH
                     GDNEGAIKSLRKYVDLATRDIYPIIIHGDDFFNKLDRWISEIGTGITRDDTIVKAGIV
                     AAIKNNPMFSVLSENKEYKFLIEKLSLLEE"
     gene            complement(3565..3705)
                     /locus_tag="ACOIAF_002845"
     CDS             complement(3565..3705)
                     /locus_tag="ACOIAF_002845"
                     /inference="COORDINATES: protein motif:HMM:NF021468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HGZ2669848.1"
                     /translation="MNGEGCILSNKITKECYKVGYMIREKSIDTYPDSGWGFMTWDEN
                     GE"
     gene            complement(4015..5322)
                     /locus_tag="ACOIAF_002846"
     CDS             complement(4015..5322)
                     /locus_tag="ACOIAF_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HGZ2669849.1"
                     /translation="MDISRSVDNSLNRTIEFLKELIKIDSQQGEPISQCPFGIGPKKS
                     LDKTLDYCASLGFSVKNIDNYIGYAEIGEGEELIGIPMHLDIVPPGEGWSVDPFSGAV
                     IDNIIYGRGVIDNKGAVSMLIHVLKNIEDMYPTINKRIRLIFGTNEETGMKCIKYYLD
                     KGEEIPSMGFTPDAMYPVVNGEKGRVHIRIEKEIKIDKSKPYIIVRGGTKENVVPSHC
                     TAKIINGIISELTTKGVAVHASNPEKGENAISKMVIKIVEDNMDFQHREDIELVSKYL
                     CSDYYGDALGINQYDEVFKNTTLNLGILKVNEEKIVCELDIRYGKNIVLNNIIDRFKK
                     VFCNGWKIEVIAHKDLHYVDESNLVLKKLLEAYEEVTDENGYTIAMGGGTYASWFKDM
                     VAFGPKFLAYKTGGHGVDERVPINHIRKNMEIYTLALIKLLEL"
     gene            complement(5387..5866)
                     /locus_tag="ACOIAF_002847"
     CDS             complement(5387..5866)
                     /locus_tag="ACOIAF_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HGZ2669850.1"
                     /translation="MSEKMYTEKEYLEGIRGATGERAVWFYLLMKEAEKLGANPDDIC
                     KEAIYGFGKMRGQKYNVADTPGKMAEMLYNSKGQKVFEMELVENTDENGVLKFHHCPL
                     DAAWKEYGLTKEERKEICRLACYGDYGRVDCAQGVKLDFAQKCAHDDEVCELVFTKK"
     gene            complement(5913..6776)
                     /locus_tag="ACOIAF_002848"
     CDS             complement(5913..6776)
                     /locus_tag="ACOIAF_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HGZ2669851.1"
                     /translation="MQEIKNKEVKNGQGPTEVNKTRVLTKKDVTKTYLRWWWTAELSN
                     SFERMQALAVCASFTPALEKLYKRKEDLVDALKRHLQFFNTQAIWGGLIHGTVLAMEE
                     EKATEGKIPGEVISGVKNGLMGPLAGIGDTLDFGTFQTIFLALGASFGAEGSVIGAFF
                     PIMFSILLFCEGYYLFHLGYSLGRDSIKKILSGGIVNKIIDGASILGMFMMGALSATT
                     VKLSTPLSFDIGGKAIVVQDTLNMIAPGLLPLGVVFFVYWGMKYKKWTITKLLVILVV
                     LALVGSFIGIF"
     gene            complement(6776..7531)
                     /locus_tag="ACOIAF_002849"
     CDS             complement(6776..7531)
                     /locus_tag="ACOIAF_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HGZ2669852.1"
                     /translation="MESVLMLAIVTGLWYWFAAGLAGYTLFSTLKSPLFIGFSLGLLW
                     GDVTTGMIVGASIEMVYLGMVAAGGNIPSDKCLAALIAIPVALQTGVNAEVAVSIAVP
                     LGVIGVLVNNLRRTGNAVLVHKADKYAEEGNTKGIWRCATLYSLIFGFVLRFPIVFVC
                     NFFGADLVQSLLDVIPQWLMNGLTVMGGILPALGFATTIFTIGKNKFLPMFIIGFFMV
                     QYFEISITAAAIFGVCIALLITFMKEDKRVGEV"
     gene            complement(7558..8046)
                     /locus_tag="ACOIAF_002850"
     CDS             complement(7558..8046)
                     /locus_tag="ACOIAF_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HGZ2669853.1"
                     /translation="MAKITLVRVDHRLIHGQVITKWVKIAQAQKIIIVDDFLGQDEFM
                     ADIYKMAAPSGVEVVILTAEDAGQAFQNNTLGDKNIFILFKNVDMANKAYKAGLKYEK
                     IQLGGIPNEAGKKMVFTAVSLGNEDVEQLNELNENGVEIVLQVIPEESSMTYENALKK
                     FK"
     gene            complement(8064..8465)
                     /locus_tag="ACOIAF_002851"
     CDS             complement(8064..8465)
                     /locus_tag="ACOIAF_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGZ2669854.1"
                     /translation="MNKYKVIILCHGTWGKVLVEETNKNFGLSNQYEVLSLSSEKDVS
                     VFMKEVEEVVDKEDVKIIISDLYGGSTSSVAIAVAIRRGIDAINGLSLQTILIVDEEL
                     SKEDKLHTLPERIVKKNNNLCVDLIKEFKKI"
     gene            complement(8468..8965)
                     /locus_tag="ACOIAF_002852"
     CDS             complement(8468..8965)
                     /locus_tag="ACOIAF_002852"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGZ2669855.1"
                     /translation="MSLVKYCRCDDRLIHGQVIYKWVKHLGVKKIVVVDDETTNDVIA
                     KGLIKMAAPKNIDLSILTVSESRRYFYNNQADDNVFVLIKNLDTANRMIEEGINIKKL
                     IVGRIPTGIGKKKISQNVYINKKEFLLIDEFIKKNINVSIQMVPDEEEVELNQNINRY
                     KGEFI"
     gene            complement(8989..9393)
                     /locus_tag="ACOIAF_002853"
     CDS             complement(8989..9393)
                     /locus_tag="ACOIAF_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGZ2669856.1"
                     /translation="MIGIIVMTHGSFSEEIIKSCELIAGPAERTAAIKLNRNDNIEDL
                     NKNFVEKLNELDEGDGVLVLADLLGGSPSNVASLNLKKGGKFHALTGVNLPMLLEALI
                     NREGKSLEELAEACIEAGQTGINNINKVLSSM"
     gene            complement(9624..12191)
                     /locus_tag="ACOIAF_002854"
     CDS             complement(9624..12191)
                     /locus_tag="ACOIAF_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HGZ2669857.1"
                     /translation="MTQKKLSRKEKVLICLKRLTEEHLNFNNGIRFGFDAEYIATEVG
                     INRSNASKELNQLIKESIIIKIKGKPVQYLYKESIESILGKKLNKGIFDNINSINEYS
                     ENDSIKDEYILESIKKENNIFNLVGCDGSLRSQIEQAKAAVIYPPKGLSTLIIGPTGV
                     GKSIFAEYMYKYSLTIKEDSDNAPFIIFNCADYSDNQQLLLAQLFGYVKGAFTGADKD
                     KYGIVHEANNGVLFLDEVHRLSAEGQEMLFLLMDKGIYRRLGEANKVHECRVMIIAAT
                     TEDPETAMLETFLRRIPVTIKIPSLEERGFQERMKLICYFFKEESVRIGVKLKVSKEV
                     IKAFILYKCKGNIGQLKSDIQLICARAFLDYMTYKRECVYVRLSLLPNNIRESLYGNN
                     RKEEFVQNFSFIGKDDIIFLPEEKDFESENLLIENKKYDLDFYGMIKETWNKLQQEGI
                     KESQIRSVIDGDIQKYSYNLMNTFIYNSSNQTAYNNIVNDSIATTVKYILQKHDKWSK
                     REGLDKLIKAIALHIQNLIERIKIGNIVKHPNKDEIMKERAYEYNIAKEILSNMSLMY
                     GVEFPEDEAIFLATFLYLSYVGLNEESIAILVIMHGESTASSMVNVANTLLDCNHAVA
                     INMGLEDRVQDILVQAVEIANKIDKGKGILILTDMGSILTFAKVIRDATGKEVKAIDM
                     VSTPIVIEATRKALTPEMTLEKLYFNIIESIKKHYGDMEVTYAEKDNGGRYFDRLLID
                     NISKTLTFLNGEKAYFILKEVINKISEHYSLVIQDELLVKFIFHCSCMIERVIIKESL
                     VYKSYEERISRNKELYLVIKESFKLVEETFDIIISDMEYANILDIFESQYDTDKFKS"
     gene            12357..13100
                     /locus_tag="ACOIAF_002855"
     CDS             12357..13100
                     /locus_tag="ACOIAF_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HGZ2669858.1"
                     /translation="MNFLSDLHTHSIVSGHGYSTLLENINYCKENGIKILGTSEHGPK
                     MPGSPHKWYFHNIQNIPRIINNIIILRGCEANILDIKGNIDLEPFVIPRLDYLILSFH
                     EAVFSPNTLENNTKALINAINKHDNIEILGHLGNPNYPIDYELIIKLAIEKNILIEIN
                     NCSIKGVSRNGSSDNCKYIATLCKKYGAKIILTSDAHICFDIGNYEYSENILKEINFP
                     DELIMNYPKKLINHFHQKGKLLDVDYTNI"
BASE COUNT         4529 a         2498 c         1424 g         5284 t
ORIGIN      
        1 tgaaacttaa gtccaagtct ttttgatttt taggtgtagt ttttaacaac ataagattac
       61 tttaccttgt gataaaggta ttattaaact ggttttttat taaataaaat aacagctgct
      121 agtaaacata ctacagaaat aagaactgtt ataataattg gaataataaa ttctggcatt
      181 gccactttcc ctgaaatcaa gtctatattt tttgacatca agaaattagg agtataaggt
      241 gcaatctgtt taggaataga aattaaactc aataaaagag ttgccaccaa tacaagtaaa
      301 acactggcaa atgctggtct aaatagtaca cacccaagaa ttaatacact aatatacata
      361 attcctataa gccataaatt aaatgctgaa aatataatat gattaagatt tgcagttgcc
      421 caaaaatata ttgtatagcc ataagcaaat aaaaaactca accaaaaacc aattgtcata
      481 ataatcaccg taacagagaa tttagacaat actatagatg aacgtgataa acctttagtc
      541 agcataataa caagtgtccc tttagaatat tcatttgata aaatattaca aaaaataatt
      601 agagttaaac tcataccaag accagagata ttgctaaaaa attgtgtcca agaatctaaa
      661 gcagttggtg cagcagtaac ttttaaagtc ggtgcaaaat gagcaatcaa gtctggcata
      721 aattttgcag aaataggact acttatacca aatattagaa ataaaataat taaactaaat
      781 aatttataat ttcttaaatt ctctttaaat tctttttttg taaatgctat gtattctctc
      841 actttactac ctccaaaaat aaattttcaa gtgataattc tattagttcc atctttactg
      901 gacaaagatt catttctgcg agtgtagata taactgtttt ttgtattgct tttatatctt
      961 taccatgcaa aacaatttcc atattagttt cttctgaatc ttttagtaag gatttaatac
     1021 catcactaga cttaaacttt tgaatttcat cattacttgc aaactccagt aaaagtctat
     1081 cctttccgtg catacttttt atttctgaaa gtgtaccact aagggcaatg cttcctttgt
     1141 ttaatatagc aacatggtca cagattcttt caacatctga tagtatatgt gttgaaaata
     1201 ttacagttgt tgagtctttg attttaagca taatatctaa tatttctttt cgaccaacag
     1261 ggtcaagtgc acttgttggt tcatcacaaa taagtaattt ggggcgtgaa agcaaagctt
     1321 gagctatacc aagtcgttgc ttcattccac gagaaaaccc acctattcgt ctcttttcat
     1381 ttccaagacc cacaagtgat aacaactctt cactccttat ctgtatttct ttttttgaaa
     1441 gtcccgtaat ttcaccacaa agagaaagat attcaagtgg tctcatataa ttgtaaaact
     1501 ctggaacatc aggcagataa cctacatgac gattagaact tgttttccca taggtcactt
     1561 tctcaccaca aacatctata gttccactat ctggtttaag taacccaagt accattttca
     1621 ttgttgtagt cttacctgca ccattcttac ctacaaaccc aaagactgac ccttctggta
     1681 cagtaaaatt taaattatct attatctttc gattaccaaa accttttgat aatccattta
     1741 ttgagacaat attcatcatt cttcacctct tcctattgta aaatacaaaa ttggtcctat
     1801 tagactaata aacagtacta caataatcca tattgcctta tttccaattt tataatttgg
     1861 atgtctcaac acatgaacaa gtgcagtaat tataagtatt aaatctaaaa taataacagg
     1921 aaccaaaagt ggtaagtatt ccatcaaatt ttccataatc ttattcctcc aataaagata
     1981 atttttctat aagaaatttg tattctttgt tttcacttaa tacagaaaac attggattat
     2041 ttttaatagc agcaacaata cctgctttta cgattgtatc atcacgtgta atacctgttc
     2101 caatttcaga aatccatctg tcaagtttat taaaaaagtc atccccatgt attataattg
     2161 ggtatatatc acgtgttgct aaatctacat attttcgtaa tgatttaata gctccttcat
     2221 tatcaccatg tattaaatta atttgtgcct gtgttaaata tgaagaaagc ataatagcag
     2281 gagataatgt atcaagctca aatatatctg aaattgctag aatacgtctt tctatttcct
     2341 tcatttgtaa tttatctgca ttttgcaaaa tacccactag attttggact aatgatataa
     2401 ggttctgata tgcaccaagt tgaaatgttt ctcttgcttc atccatttgg ccattcatca
     2461 tttgcccctg tgcaagaagt atagattcat ttataatggg aaaattgctg ttttgcaata
     2521 aatcaatcac ttgtatgggt tgatttaaca atatataaca agttgcctcc atactttttg
     2581 cttgacggca aatatctatg tcattacaag tttcctgtat acgaataaat atttctaatg
     2641 cttcactaat taatagttca cgcttttttt catctacaat atcatagtga ttaactatta
     2701 aaagacccat ccgaaaaatc aaaggaaaac atgaataata tttttttatg atttctctta
     2761 tttcatccat gacttcatca aatggtttaa ccgtaaagtc tttgcacagt ttattataca
     2821 gtttattaat atcttccttc atcatttgag gttcataaca tattaactca tcaacggtaa
     2881 tgttgaaata tgtcgcgagt tgcggtaaaa aggttatgtc aggatagctt tgtccagttt
     2941 cccatttaga tacagaagct ttagaaacgc ctatataatt tgcaagttca tcttgtgtaa
     3001 ttcctttttc tttacgcttt aaaatcagcg tttttgcgat gtttaattca ttcatgtcaa
     3061 tccctcctta tgtataatta tatccttaaa catgtaaagc taacaagcga tttgaagttg
     3121 acttttgacg aaaaaatcaa ccacagagaa acaaataaac aagtatataa aatatatatt
     3181 taaatttatc tttaatctct tttgtaaaat atgtaaactt tgtaaataaa atccatatta
     3241 tataaaacta atagttaatc tatctaaata cagtttttat ttatcacgtt tttaaatatt
     3301 ttgtgtaaaa cctcttttaa tataaacacc ttattttcat ttctcatttc atattctttt
     3361 taaaatactg aatataactc ttcgattttc catttaattc ttttataaag ttattattcg
     3421 tatttatttc tctaattata acaggcaact atcatgtatt ggtatttgtc tattatataa
     3481 aaatctataa aagaaaagca gtcaatcatt tagattaact gcttgtacat atatcttatt
     3541 aaactgtata aactataatt tttattattc tccattttca tcccaagtca taaatcccca
     3601 tccactatct ggataagtat caatagactt ttctctaatc atataaccaa ctttataaca
     3661 ctcttttgtt attttattag atagtataca tccttctcca tttatctaat tccattcaat
     3721 taacttttct tttcaacctt aacttttata aaatcatctt ttttaataat ttcatactta
     3781 ttttctcttc aacatcatca aatgagggtg tctcaaaatt aactttttac ttcataaggc
     3841 actctttttt gtatgaagta aaatatattt tcatcatttc aacaataagt aagaggctta
     3901 tctctatttt gagacagccc catttgatgg tgttgaacat ttaaataagg ttgaactcgt
     3961 tttatctctc tttttaagat atctgtaaat ttgcacataa agtctcttct tcttttacaa
     4021 ttctaatagt tttattaaag ccaaagtata tatctccata ttctttcgta tatgatttat
     4081 tggtactctt tcatctactc catgaccacc agttttatac gctaagaact taggtccaaa
     4141 cgccaccata tctttaaacc aacttgcata tgtgccacca cccatagcaa ttgtatatcc
     4201 attttcatct gttacttctt cataggcttc taataatttt ttaagtacta aattactctc
     4261 atccacataa tgtaagtctt tatgagcaat aacctctatt ttccaaccat tacaaaaaac
     4321 ctttttgaat ctatctatta tattgtttaa aactatattt ttaccatacc ttatatctaa
     4381 ctcacatacg attttttcct catttacttt taatattcct aaatttaagg tagtgttttt
     4441 gaatacctca tcatattgat taatccctaa tgcatctcca taataatcac tacaaaggta
     4501 ttttgaaact aattcgatat cttctctatg ctgaaaatcc atattatcct caactatctt
     4561 tattaccatt ttagatatag cattttctcc cttttcagga ttacttgcat gtacagctac
     4621 tcctttagta gttaactctg aaataattcc attaataatt tttgcagtac aatgagatgg
     4681 aacaacattt tctttagttc ctccccttac tattatgtaa ggtttacttt tgtcaatttt
     4741 aatttctttt tctatcctta tatgaaccct tcctttttct ccattaacca ctggatacat
     4801 agcatctgga gtaaatccca tacttggaat ttcttcacct ttatctaagt aatatttgat
     4861 acatttcata cctgtctcct catttgtacc aaaaattaat cttattcttt tattaattgt
     4921 aggatacata tcctctatgt ttttcaatac atgtataagc atagatacag cacccttatt
     4981 atctattaca cctcttccat aaataatatt gtcaattact gctcccgaaa atgggtctac
     5041 actccacccc tctcctggtg gaactatatc taaatgcatt ggaatcccta ttaattcctc
     5101 tccctctcca atttctgcat atccaatata attatcaata tttttaacac taaaacctaa
     5161 actagcacag taatctaaag ttttatctaa agatttttta ggtcctatac caaaaggaca
     5221 ttgtgaaata ggttcacctt gttgtgaatc aatttttatc aattctttta gaaattctat
     5281 ggttctattt aaactattat ctacacttct gcttatatcc ataaatcaac ctactttcta
     5341 tctttttaaa gcctgtcctt aaagaacagg cttttagcat taaaaattat ttttttgtaa
     5401 atactaattc acaaacttca tcatcatgag cacatttttg agcaaaatct aatttaacac
     5461 cttgtgcgca gtcaactctt ccataatccc cataacaagc taatctacaa atttcttttc
     5521 tttcttcttt agttaatcca tattctttcc aagcagcatc taatggacag tgatggaatt
     5581 ttaaaacgcc attctcatct gtattttcta ctaattccat ttcaaacact ttttgtcctt
     5641 tactgttata taacatttct gccatttttc ctggagtatc tgctacgtta tatttttgac
     5701 ctctcatttt accaaatcca tatattgctt ctttacatat atcatctgga ttagctccta
     5761 atttttcagc ttctttcata agaagataga accatactgc tctttctcct gttgctcctc
     5821 taattccttc taaatattcc ttttctgtat acattttttc tgacatttct ttctcctcct
     5881 aaaattaaat tctatatttt aatcactgtg aattagaata ttccaatgaa tgaccctacc
     5941 aatgctaata caactaaaat tactaataac ttagttattg tccacttctt gtatttcatt
     6001 ccccaatata cgaagaatac aacaccaagt ggtaataaac ctggagcaat catatttaat
     6061 gtatcttgaa caacaattgc tttaccacca atatcaaatg atagcggagt tgaaagctta
     6121 actgtagttg cagataatgc tcccatcata aacattccta gaattgaagc accatcaatt
     6181 atcttattga caataccacc tgatagaatt ttcttaattg aatctctacc taaagaatat
     6241 cctaaatgaa ataagtaata accttcgcaa aatagtaata tactaaacat tattgggaaa
     6301 aatgcaccta taacacttcc ttctgctcca aatgaagcac ctaaagctaa gaatattgtt
     6361 tgaaatgtac caaaatctaa agtatctcca attccagcca aaggtcccat aagaccattc
     6421 ttaacacctg aaataacttc tcctgggatt tttccttcag tagctttctc ttcttccata
     6481 gctaaaactg taccgtgaat taatcctccc catatagctt gagtattaaa aaattgtaag
     6541 tgtctcttta acgcatctac taaatcttct tttctcttat ataacttttc taaagccgga
     6601 gtgaaacttg cacaaactgc taatgcttgc attctttcaa aagagttgga taattctgct
     6661 gtccaccacc atcttagata tgtttttgtt acatctttct ttgtaagtac tcttgtctta
     6721 ttaacttcag ttggaccttg tccattttta acttctttat tttttatttc ttgcattata
     6781 cttcacctac ccttttgtct tctttcataa aggtaattaa aagagctata caaacaccaa
     6841 atattgcagc tgcagtaatt gatatttcaa aatattgaac cataaaaaat cctataataa
     6901 acattggtaa aaatttattt ttacctatag taaatattgt tgttgcaaaa cctaatgcag
     6961 gtaatatacc gcccattact gttaatccat tcattaacca ttgtggtata acatctaata
     7021 atgattgaac taaatctgct ccaaaaaagt tacatacaaa aactattgga aaccttaaaa
     7081 caaacccaaa tattaaagaa taaagtgttg cacatctcca gatgcctttt gtattacctt
     7141 cttcagcata cttatctgcc ttatgaacca atacagcatt accagttctt cttaagttat
     7201 taactaatac tcctataaca cctaaaggta ccgcaataga tactgcaact tctgcattaa
     7261 caccagtttg aagtgctact ggtattgcaa taagagcagc tagacattta tctgatggaa
     7321 tatttccccc tgctgcgacc attcctagat aaaccatttc tatacttgca ccaacaatca
     7381 taccagttgt tacatcaccc caaagtaatc caaggctaaa acctataaat aaaggagact
     7441 ttagtgttga gaaaagggta taccctgcaa gacctgctgc aaaccaatac cataatcctg
     7501 ttacaattgc caacatcaaa acagattcca tttataattc ctcctaattt atatttttta
     7561 tttaaatttc tttaacgcat tttcatatgt catagatgat tcctctggta taacttgtag
     7621 gactatttca accccatttt cattaagttc attaagttgt tctacatcct catttcctaa
     7681 tgaaacagct gtaaacacca ttttctttcc tgcttcattt ggaatacctc ctaattgaat
     7741 cttttcatat ttaagtccag ctttataagc cttatttgcc atatcaacat ttttaaatag
     7801 aataaatata tttttatctc ctaaagtgtt gttttggaat gcttgtcccg catcttctgc
     7861 tgttaaaata actacttcta ctcctgatgg tgctgccatt ttatatatgt cagccataaa
     7921 ttcatcttga cctaagaagt catcaactat tattattttt tgagcttgag cgatttttac
     7981 ccattttgtt ataacttgcc catgaattaa tctgtggtct actcttacta atgttatttt
     8041 tgccattata ttcacctcat aatttatatt tttttaaatt ctttaattaa atccacacaa
     8101 aggttattat tttttttgac gattctttct ggtaatgtat gtaatttatc ttctttgctt
     8161 aattcttcat ctacgattaa aattgtctgt aagctaagtc catttattgc atctattcct
     8221 cttcttatag caacagctat tgcaacactt gatgtagatc ctccatataa atctgatatt
     8281 ataattttca catcttcttt atctactact tcttcaacct ctttcataaa tacagaaaca
     8341 tctttttcag aacttagtga taaaacttca tattgatttg acaatccaaa gtttttattt
     8401 gtttcttcta ctaagacttt tccccaagtt ccatggcaca gtatgattac cttatattta
     8461 ttcattgcta aataaactct cctttatagc gattaatatt ttggtttaat tctacttctt
     8521 cttcatcagg taccatttga attgatacat taatattctt tttaataaat tcatctatta
     8581 aaagaaactc ttttttatta atgtatacat tttgagatat tttctttttt cctattcctg
     8641 taggtattct tccaacaata agttttttga tattaatccc ttcttctatc attctatttg
     8701 cagtatctaa atttttaatt aatacaaaaa cgttatcatc agcttgatta ttataaaaat
     8761 atctcctcga ttcacttaca gttaatattg ataaatcaat attctttggt gctgccattt
     8821 ttatcaaacc tttagcaata acatcattgg ttgtttcatc atctactact actatctttt
     8881 ttacacctag atgcttaacc catttatata taacttgacc atgtattaat ctatcatcac
     8941 atctgcaata ttttactaaa gacattttta atcaaactcc tttttctatt acattgagct
     9001 aagaacttta ttaatattgt ttataccagt ttgtcctgct tcaatacatg cttctgctaa
     9061 ctcttctaaa ctttttccct ctctattaat taaagcttca agtagcattg gaagatttac
     9121 tccagtaagt gcatgaaact tgccaccttt tttaaggttt aaacttgcaa cattacttgg
     9181 cgaacctcct aataggtcag ctaatactaa aactccgtca ccttcatcta attcatttaa
     9241 cttttctaca aaatttttat ttaagtcttc aatattatca ttcctattta gtttaattgc
     9301 tgctgttctt tctgctggtc ctgcaataag ttcacagctt tttataattt cttcactaaa
     9361 actcccatga gtcataacta ttattcctat caccaatttc acctcacttt tcttgtcact
     9421 ttttatataa gcaattgccg tgccaactta tgtgtatcaa taacaaatgg ctattttaca
     9481 ggatttaatc aaaacttttt actcaatttt ttttaaaaac ttattatttc gatatgtatc
     9541 aaaagatttt ttcaacctgt atcaaatctg tatcaaaaaa agtaaaaaaa aagatagcct
     9601 tttggctatt tttaaatttt gtatcaactt ttgaatttat ctgtatcata ttgtgattca
     9661 aatatatcta atatatttgc atattccata tctgaaataa ttatatcaaa tgtctcttct
     9721 actagcttaa aactttcttt tataactaaa tataattctt tatttctact aattctttct
     9781 tcatatgatt tataaactaa agactctttt attattactc tttcaatcat acatgaacaa
     9841 tgaaatataa actttactaa aagttcatct tgaattacta aagaatagtg ttcactaatt
     9901 ttattaatta cttcttttaa tataaaataa gccttttccc catttaaaaa agtcaaagtt
     9961 ttactaatat tatcaattag taatctatca aaatatctac caccattatc tttttcagca
    10021 tatgtaactt ccatatctcc ataatgtttc tttatagatt ctataatatt aaaatataat
    10081 ttctctaatg tcatttctgg agttagagcc tttctagttg cttctattac aataggtgta
    10141 cttaccatat ctattgcttt tacttctttt cctgtagcat cacgaataac tttagcaaat
    10201 gttaaaatag accccatatc agttaaaatt aaaattccct ttcctttatc aattttatta
    10261 gctatttcta cagcttgtac taaaatatcc tgaactctat cttctaatcc catattaatt
    10321 gccactgcat ggttacaatc cagtaaagta ttggctacat tcaccatgct agatgctgta
    10381 gattcaccat gcattatgac taatattgct atactctcct catttaatcc cacatacgat
    10441 aaataaagaa atgttgctaa aaaaattgct tcatcttctg gaaactctac tccatacatt
    10501 aatgacatgt tagataatat ttcttttgct atgttatatt catatgctct ttccttcata
    10561 atttcatctt tattaggatg ctttactata tttccaatct ttattctttc aattaaattc
    10621 tgaatatgta aagctatagc ttttattaat ttatctaatc cttctctttt gctccattta
    10681 tcatgctttt gtaaaatata ttttacagta gttgcaattg aatcattaac aatgttgtta
    10741 taagctgttt gattactact attatatata aaagtattca ttaaattata ggaatacttc
    10801 tgtatgtctc catctataac acttctaatt tgtgactctt taataccttc ttgttgaagt
    10861 ttattccaag tttcttttat cattccataa aaatctaaat catatttttt attttctatt
    10921 aataaatttt cactttcaaa atccttttct tctggtaaaa aaataatatc atcttttcca
    10981 ataaaactga aattttgtac aaactcctct tttctattat ttccatataa tgattctcta
    11041 atattatttg gtagaagaga gagccttaca tatacacatt cccttttata ggtcatataa
    11101 tctaaaaaag ctcttgcaca aattaattgt atatcgcttt ttaattggcc tatgtttccc
    11161 ttacacttat ataaaataaa tgccttaata acttcttttg aaacctttaa cttaacacca
    11221 atcctaacac tctcttcttt aaaaaaataa catataagct tcattctctc ttgaaagcct
    11281 ctttcctcta aagacggaat ctttatagtt acaggtattc ttcttaaaaa cgtttccaac
    11341 attgctgttt ctgggtcttc tgtagtggct gcaattatca taactctaca ctcgtgtacc
    11401 ttattagcct ctcctaacct tctatatatc cctttatcca taagcagaaa tagcatttct
    11461 tgtccctctg cagataatct atgaacttca tctaaaaaca atactccatt attagcttca
    11521 tgtacaatac catacttatc tttatcagct ccagtaaatg ctccttttac atatccaaaa
    11581 agctgcgcta ataataattg ctgattatca gaataatcag cacagttaaa tatgataaat
    11641 ggtgcattat cactatcttc ttttatagtt aaactatact tatacatata ttctgcaaat
    11701 atagatttac caactcctgt aggtcctatt attaatgtac tcaacccctt aggtggatat
    11761 attactgctg ctttagcctg ttctatttgg gaccttaaac ttccatcaca tcctactaaa
    11821 ttaaaaatat tgttctcttt ttttatactc tctaaaatat actcatcttt gatagaatca
    11881 ttttcactat attcgtttat agagttaata ttatcaaata tccctttatt tagtttcttt
    11941 cctaaaatac tctcaatact ttctttatat aagtactgaa caggcttacc ctttatcttt
    12001 ataataatac tttcttttat taattgattt aattccttac tagcattact tctatttata
    12061 cctacttcag tagctatata ttctgcatca aatccaaatc taatgccatt attaaagttc
    12121 aagtgttctt ctgttaacct tttcaagcaa attaaaactt tttctttcct tgatagcttc
    12181 ttttgtgtca tagttaacct cccctataag cagtaatact gttaaacatt tattattaag
    12241 cttaattcta caatatttta atatttttgt ctaattatgt aactattgat tttctttcct
    12301 attcgtttta taatataaat tacgttaccc tcatctttta tagaggagga atttatatga
    12361 attttttatc agatttacac acccactcta tagtcagtgg tcatggctat agcactttac
    12421 tagaaaatat taattattgt aaagaaaatg gaattaagat actaggtaca tctgaacatg
    12481 gtcctaagat gcctggttct ccccacaaat ggtactttca caacattcaa aatatcccaa
    12541 gaatcataaa taacattata attttaagag gctgtgaagc aaatatacta gatattaaag
    12601 gaaacataga cctagagcct tttgtaattc ctcggttaga ttatcttatc ttgtcatttc
    12661 acgaagctgt gttttctcca aatacattag agaataatac taaagcttta ataaatgcaa
    12721 taaataagca tgataatatt gaaatattag gacaccttgg aaacccaaac tacccaatcg
    12781 actatgaatt aatcattaaa ttagctatag aaaagaatat cttaatagaa ataaataatt
    12841 gttctattaa aggcgtgtca agaaatggta gctcggataa ctgtaagtat attgctacac
    12901 tttgcaaaaa atatggtgct aaaatcatac tgacttcaga tgctcacatt tgttttgaca
    12961 taggtaacta tgagtattct gaaaatatct taaaggaaat taattttcca gatgaattaa
    13021 taatgaatta tcctaaaaaa cttattaatc attttcacca aaaaggtaaa ttactagatg
    13081 ttgattatac aaatatctaa gtaataatac atgcttactt taactcccat aaaaatttta
    13141 atactattta tattatatga aataaagaaa ttataataaa gaagctattt aataaagctt
    13201 aattactttt atgaacttga tggattgata tggtctatga tagcaataga aattataaca
    13261 tttttaataa acttagtttt atatacctta tagtataaat ctaaaaaagc atcatctaaa
    13321 aaggagctgt cacatgagca aattaaattt gttctatgat agctcttttt ttacttattt
    13381 taattaggta tatttttaat tattttatat aaagcttatg ttctaagtaa aattatgcat
    13441 aacaaacaaa ggtataaaaa taccattttt tcagataata tttaattata aataataaat
    13501 ttagaatact tcttttaaat gatgagagtc tagaaatttt tatgtgtatt ctttaaataa
    13561 aaatttccag actctagccc ctttttgaat attttaaaat ttaacttaaa ctaagcaaat
    13621 tctataatag cttatctcta aatatttaaa tttaatttat catacaatat tacttattat
    13681 aaaacttaaa acatataaaa atatatttta aagcaataat tttattttac ttatt
//
LOCUS       DBGJFF010000102        13445 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.103, whole genome shotgun sequence.
ACCESSION   DBGJFF010000102 DBGJFF010000000
VERSION     DBGJFF010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13445
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.103"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            214..2073
                     /locus_tag="ACOIAF_002856"
     CDS             214..2073
                     /locus_tag="ACOIAF_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HGZ2669859.1"
                     /translation="MKRFLRRIILVLFILLIFISFISIKLIHNVGDYGKLINYVGIVR
                     GASQRLTKLEMNHQPNDELIEYIDEILQELITGHGDYGLVITDCNEYNEDLLLLEKKW
                     EDLNIEIKKVRMKEQNNQLLSISEEFFSLANDTVFKIENFSKEKSNYLMTLIIIISII
                     GILACIILILQYSKKMVRLEKLNVDLKNIAYKDELTGVNTIEKFKLDANQNICIHKDK
                     KFAVFYIDFENFKYINDIFGYDYGDMILKRYANLMMNDIGKYEIFAREIADRFVALRC
                     YVDKEDLVVRQRDVDNELINITNEIKNKHSITVVSGICCIEDVNEKLSIDGLINRANF
                     AQKTVKNKPGTNYAFYNDSIRKKMIEENTIKSRIHEAIEKREFIVYLQPKVNLHSQKI
                     NCAEALVRWLTPDKGIISPAIFIPVLEKNFFIALVDKYVFEEVCKWIRKRLDENKPFV
                     QISVNVSRIQFYNTKFVETYSNIQNKYRIPKNTIEIEFTESVAFENQKHLLEIIHDLH
                     ENGFTCSLDDFGKGYSSLSVLKDLPFDVLKLDSMFFKASLDKDKEKIVIKNIVHMLKE
                     LNITTVAEGIECKEQVEFLRDIGCDLVQGFVFYKPMPILEFEKILDKEFVYNS"
     gene            complement(2365..2964)
                     /locus_tag="ACOIAF_002857"
     CDS             complement(2365..2964)
                     /locus_tag="ACOIAF_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HGZ2669860.1"
                     /translation="MAIELKKGQKINLTKKENSDLGEILVNLNWNQKVQKKGFFGSLR
                     SSNIDLDLGCLFDMKNGVKGAVQALGNAFGSLDNPPFAQLDGDDRTGSNTQGENLRIN
                     GNKIKDIKRILIYAFIYEGVANWSEADGIVTIKQKQDSDLVVKLDEHKNGYNMCSIAL
                     IENVNDETFSVEKVVKYFKGHREMDTEFHWGLKWVAGRK"
     gene            complement(3067..3864)
                     /locus_tag="ACOIAF_002858"
     CDS             complement(3067..3864)
                     /locus_tag="ACOIAF_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HGZ2669861.1"
                     /translation="MKFKKLLCLLLCLVLTLAVVGCSKAKDDKKIVVGATLVPGGELL
                     EELKPLIKEKGYTLEVKNFDDYILPNEALNNGEIDANLFQHEPYLKEAVKAKGYKIMA
                     GKKLYVCPAILYSYKIKSVDEFKKGDTIAISNNPSSCSKNLRYLESIGLLTLPKGDGL
                     VSPKDIIENPKGIQFKELDIAQIPSSLPDVTAAFIDTTYAVPAGLDAKKNGIYTAPIN
                     DEYANLLAFRTEDKDSEKIKVLQDVLTSDKARSLIEEKYKGIVIPTF"
     regulatory      complement(3977..4082)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(4174..5160)
                     /locus_tag="ACOIAF_002859"
     CDS             complement(4174..5160)
                     /locus_tag="ACOIAF_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HGZ2669862.1"
                     /translation="MLLIYNEKTNPYFNLAMEEYLLKNFDEDLFILWRNEPSIIVGKN
                     QNTLSEINLEYIKEYSIPVVRRQSGGGAVFHDLGNINFTFIACNNNNFSDFRRFTQLI
                     IDLLKTLDVNAEFSGRNDLLIDGKKFSGNAQYNYKNKVMHHGTLLFSSEINDLSKALK
                     VKPIKFEGKGIKSVKSRVTNISEHLHSKMDILEFKDAIMDYLSKTNTDNTNYCLSEHD
                     IKQIEKLTKSKYETWDWNFGNSPKYTLSNELKYSGGNVEFNLNVDKGIITSIKFFGDF
                     FGKCDVAFVENKLIGIRHEENALRNTLDSIEINDYFLGADTDILVSGILGVK"
     regulatory      5324..5500
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            5644..7374
                     /locus_tag="ACOIAF_002860"
     CDS             5644..7374
                     /locus_tag="ACOIAF_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HGZ2669863.1"
                     /translation="MRNKKINIIFLTTIMFIMSTVMVFAEEDIDTIALANAEKFGILT
                     LIPPLVAIILAFITKNVIISLLIGILSGSFIIKASGINVFATFIQAFLDLVDRALVSL
                     ADPWNAGIILQVLAIGGVINLVAKMGGAKAIAEALAKRAKSAKGTQLITWFLGLLVFF
                     DDYANSLIVGPMMRPVADKMKISREKLAFIIDATAAPVAGLAIISTWIGLEVGLIHDA
                     FESISIDVDAFGIFLNTIPFRFYNILILAFIVISALLLKEFGPMRKAEIKSRSRKISI
                     DLDEGVEELDDLAPKNGVKLSVWNAIIPIGTLIIVALASFYYSGYTSIMGGDDKALIQ
                     LFTNSPYSFEAIKEAFSASDASRALFQSALVASLVAIIMAVVKKIFTISEAIDVWIDG
                     MKSLVITGVILILAWSLSSVIKELGTAKFLIHLLSGSLPPFLLPSLIFGLGAIISFAT
                     GTAYGTMGILMPLAIPLAYSLNPDMSYVIVSTSAVLTGAIFGDHCSPISDTTILSSMG
                     AGCNHIDHVNTQMPYAIFTAVITIVFGYIPAGLGLPIYIVLPVAIAAIFVGIQIIGKK
                     VDEAEIELVE"
     gene            7484..7564
                     /locus_tag="ACOIAF_002861"
     tRNA            7484..7564
                     /locus_tag="ACOIAF_002861"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7518..7520,aa:Leu,seq:tag)
     gene            7899..8414
                     /locus_tag="ACOIAF_002862"
     CDS             7899..8414
                     /locus_tag="ACOIAF_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
                     /protein_id="HGZ2669864.1"
                     /translation="MEKYIWLFPLLFIFHDMEEIIGFGIWLKKNKSMLDKKYPFISKV
                     YENYSTEGMAFAVFEEFILCIIFCILTVITENQYVYLLWLGSFIAYTLHLVIHIGQSI
                     IIRKYIPSLITSIICLPISIWCISKSIYIVDCEMSTTILYSIIGIIIVALNLKFAQSL
                     IGKFTKWMSNI"
     regulatory      8906..9010
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            9220..11694
                     /gene="gcvPA"
                     /locus_tag="ACOIAF_002863"
     CDS             9220..11694
                     /gene="gcvPA"
                     /locus_tag="ACOIAF_002863"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HGZ2669865.1"
                     /translation="MNELKRVSLYNIHKELGAKLVEFAGWEMPLEYEGINKEHEKVRK
                     SAGIFDVSHMGEVQIKGAESEKFIQNLVTNDISTLKINDIIYTPMCYENGGVVDDLLI
                     YKFGEEDYLLVINAGNIDKDVAWIIKQSEGYNVDIKNISSEVSQLAIQGPKAEEILQK
                     ITDIDLNSIKFYKSIPSTKVCGCPCLVSRTGYTGEDGFEIYCKNKYVEIIWNEVLKVG
                     GEDICPAGLGCRDTLRFEAALPLYGHEINEHISPIEGGLSIFVKTNKESFIGKSILSK
                     EKESGAKRKLVGFEMQGKGMPRNGYDIRIGDKTVGFVTTGCASPTTGKILGMGIIDSE
                     YAKVGNEIGIAIRKKVVPAVIVKKPFYKKQYKKDNIILNKENKFSYIPATSEDKSKML
                     KVVGLNSVDELFSDIPEEVKLKRDLNLEIGKSELEVSKIVKRLSEENLSLEDLTCFLG
                     AGAYDHYIPSIIKHITSRSEFYTAYTPYQAEISQGTLQVVFEFQSMIAEITGMEIANA
                     SMYDGATAAIEACIMAMNQTRKSKIVVSKTIHPETLSILRTYLQYKDCEIVEIDFCNE
                     YGTTDIEKLKASVDKDTACVLIQTPNFFGIIEEMEEIEKITHENKAMLIMSVDPISLG
                     VLKTPGEIGADIVVGEAQSLGNPLNFGGPYVGFLASKSKYTRKMPGRIVGQSLDVEGK
                     IAYVLTLQTREQHVRREKATSNICSNQALNALVASIYMATMGKEGFKEVGMQSMKKAH
                     YTYNKLVQTGKYKPIFKGKFFKEFAVQGNLNIETINDKLLEENILGGYNLEYNYPELK
                     NSTLLCVTEKRSKEEIDKLVGIMEGL"
     gene            11694..13151
                     /gene="gcvPB"
                     /locus_tag="ACOIAF_002864"
     CDS             11694..13151
                     /gene="gcvPB"
                     /locus_tag="ACOIAF_002864"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HGZ2669866.1"
                     /translation="MKEYNSLLIDISKKGRKAYSLPKLDIDDIKIEDMIDQDMARKSE
                     LNLPEVGELELVRHYTLLSNKNFGVDTGFYPLGSCTMKYNPKINEDMAALPNFTGMHP
                     YQSSDTAQGSLSLMYDLSRRLAEITGMDEVTLQPSAGSHGEFTGLMIIKAYHENRDDH
                     KRTKVIIPDSAHGTNPASAAMANFDVIQIASDKNGAVDINALKEVLNDEVAALMLTNP
                     STLGLFEKNIKEIATLVHEAGGLLYYDGANMNAIMGITRPGDMGFDVVHLNLHKTFST
                     PHGGGGPGAGPVGVKKELVPFLPIPVIERKEDKYVLNYDREKSIGKIKNFYGNFGVLV
                     RAYTYILTMGRDGLKEASEMAVLNANYIKESIKDDYILPIDTLCKHEFVLGGLPRGEA
                     NIKTIDIAKRLLDYGYHPPTMYFPLIINEALMIEPTESESIETLDSFIEAMKSVAKEA
                     KEEPELLKTAPHNTLVKRVDDARAVKKPILTWSQR"
BASE COUNT         5058 a         1590 c         2206 g         4591 t
ORIGIN      
        1 tataaattac attaagagta ataatctata agaacttata tattagaaaa aattttgtat
       61 atatagagga ctctaaagat gaactatgta aaacaaggtt tatatattct aattgtatag
      121 aaaatttatt gtaaacgcat gtatattatt agaaaaaata gtttgaaggt attcaataaa
      181 cataaaaaac aatatagggg ataaaggtgt tttatgaaga gatttttgag gagaattatc
      241 ttagttttat ttattttgtt gatttttata agttttattt ctattaaatt aattcataat
      301 gttggagatt atggaaaatt gattaattat gttgggattg ttcgtggagc tagccaacgt
      361 ctaactaaac tagagatgaa tcatcaacca aatgatgaat taatagaata tattgatgaa
      421 atcctacagg agttgattac agggcatgga gattatggtt tagtaatcac tgattgtaat
      481 gaatacaatg aggacttact tttattagaa aaaaagtggg aagatttaaa tattgaaatt
      541 aaaaaagtaa gaatgaaaga acagaataac cagttattaa gtataagtga agagtttttt
      601 tctcttgcaa atgatacagt gtttaaaata gaaaactttt ctaaagaaaa aagtaattac
      661 ttaatgactt taataataat tatatctatt ataggtatat tagcatgtat catattgatt
      721 ttacaatata gtaaaaaaat ggttagactg gaaaaattaa atgtagattt aaaaaatatt
      781 gcatataaag atgaactaac aggagtaaat actatagaaa aatttaaatt ggatgcaaat
      841 caaaatattt gtatacataa ggataagaaa tttgctgtat tctatattga ttttgaaaac
      901 tttaaataca ttaatgatat atttggatat gattatggtg acatgatact gaaaagatat
      961 gctaatctta tgatgaatga catagggaaa tatgaaatat ttgcaagaga aattgcagat
     1021 agatttgttg cgttaagatg ctatgtagat aaggaagatt tagtggtaag acaacgagat
     1081 gtagacaatg aactaattaa tataacaaat gaaattaaaa ataaacatag tattactgtt
     1141 gttagtggaa tttgttgtat tgaagatgta aatgaaaaat taagtataga tggtttaata
     1201 aatagagcaa attttgcaca aaaaactgtt aagaataaac caggtactaa ttatgctttt
     1261 tataatgaca gtattcgtaa aaaaatgatt gaagaaaata ctatcaagag tagaatacat
     1321 gaagctattg aaaaaagaga atttatagtt tatttacagc ctaaagttaa tttacatagt
     1381 caaaaaataa attgtgcaga agctttagtt cgttggctta ctccagataa aggtattata
     1441 tcgccagcaa tatttattcc agtacttgag aagaattttt ttattgcctt agtagataag
     1501 tatgtttttg aggaggtttg taaatggatt agaaagaggt tagatgaaaa taaacctttt
     1561 gttcagatat cagttaatgt ttcaagaata caattttata acactaaatt tgtagaaact
     1621 tattccaata tacaaaataa atacaggata cctaaaaata ctatagaaat tgaatttaca
     1681 gagagtgttg cttttgagaa tcaaaaacat cttttggaaa ttatacatga tttgcatgaa
     1741 aatggattta cttgttcttt agatgatttt ggaaaaggtt attcgtcttt aagtgtttta
     1801 aaagatttac catttgatgt tttaaagctt gactctatgt ttttcaaggc tagtttagat
     1861 aaagataaag agaaaatagt aataaaaaat attgtacata tgttaaaaga acttaatatc
     1921 acaactgttg ctgaaggcat tgaatgcaag gaacaagttg aatttcttag ggatattggg
     1981 tgtgatttag ttcagggatt tgtattttat aaaccaatgc caatattaga atttgaaaaa
     2041 atattggata aagagtttgt ttataatagc taaaatgtac tttataaaat atgaactaat
     2101 taagtaaaat tattttgtac tgaataaatt tttactttat ttacagaata atgattattt
     2161 acagaaaagc gcgaaaagtg tattccttta agtgtgggat ggatagcaca aataggggag
     2221 aaaaaatatt ggccatataa gaatgcataa taatataaat atttgaaagg tcagcatcat
     2281 ataaaaatta aaaagaaaat aaataatttt tctataaaaa agagactatg agaaaattta
     2341 ctcatagtct cctaaataat gtatttattt tcttcctgca acccatttta atccccaatg
     2401 aaattcagta tccatttctc tatgaccttt gaagtattta actacttttt caacactgaa
     2461 tgtctcatca tttacatttt ctatcaatgc tattgaacac atattatagc catttttatg
     2521 ttcatcaagt ttgacaacta agtctgaatc ttgtttttgt ttgattgtta caattccatc
     2581 agcttcagac cagttagcta caccttcgta tataaaagca taaataagta ttctttttat
     2641 atcttttatt ttattaccat ttattcttaa attttctcct tgtgtattac ttcctgttct
     2701 gtcatctcca tctaattgag caaaaggtgg attgtctaaa ctaccaaagg catttcctaa
     2761 tgcttgaaca gcacctttta caccattttt catatcaaat aggcaaccca aatctaaatc
     2821 tatattactg cttcttaaag agccaaaaaa accctttttt tgtacttttt gattccaatt
     2881 taaattaact aagatttcac ctaaatcgct attttccttt tttgtaaggt ttattttttg
     2941 tccctttttt aattctatag ccatagtaat cttcctccac aaacatagat ttatttaaat
     3001 ggtagcactt aataagtcaa tattcaacaa gattagaata ataatattaa aattatacta
     3061 aatatactaa aaagtaggaa tcacaatacc tttgtatttt tcttcaatta agcttcttgc
     3121 tttatctgat gtaagaacat cttgtagaac tttaatcttt tcactgtctt tatcttcagt
     3181 tctaaatgct aaaagatttg catactcgtc atttatagga gctgtataaa ttccgttttt
     3241 cttggcatca agaccagcag gtacagcata agtagtatct ataaatgcag cagtcacatc
     3301 tggaagagaa gatggaattt gagcaatatc taactctttg aattgtattc cctttggatt
     3361 ttctattatg tccttaggac taactaatcc atccccttta ggtaacgtta aaagtccaat
     3421 actttcaagg tatctaaggt tcttagaaca agaagatggg ttattgctta ttgcaattgt
     3481 atctcctttt ttaaattcat caacagattt tatcttataa gagtaaagta ttgctgggca
     3541 tacatatagt tttttaccag ccattatttt gtaacctttg gccttaacag cttcttttag
     3601 ataaggttca tgttgaaata gatttgcatc aatttcacca ttatttaaag cttcgtttgg
     3661 aagtatgtaa tcatcaaagt ttttaacttc taaagtatat cctttttctt taataagtgg
     3721 ttttaattct tctaataact ctcctcctgg aactaaagta gcaccaacaa caattttttt
     3781 atcatcttta gctttagaac atccaaccac agctaaagtt aatactagac ataataacaa
     3841 acataataat tttttgaatt tcatataaat tccctcctga atataattga ttagttaaaa
     3901 ataaaatacc attctttatc acactgcaaa ataaaaataa gcctgagttt aaactcaggc
     3961 tttaaaagct aatttagact catctttcag tataatactg caggatttag caccaatgta
     4021 tataaaaata tactggttgc tgggtttcat agggccagtc ccttcaccac tctggataag
     4081 aagtatcaat ttttaattat gttataaata ttatcatgat tttttacgtc tgtaaacata
     4141 attttataaa tattttaaaa tctttaatgt attttattta acacctaaaa ttccagaaac
     4201 tagaatatca gtatcagcac ctaagaaata gtcatttatt tcaatagaat ccagagtatt
     4261 tctcaaagca ttttcttcat gccttatacc aataagttta ttttctacaa atgcaacatc
     4321 acattttcca aaaaaatctc caaaaaattt tatactagtt attatgcctt tatcaacatt
     4381 taaattaaac tcaacatttc caccagaata ttttaattca tttgaaagag tatattttgg
     4441 tgaattacca aaattccaat cccaagtttc gtattttgat ttagtaagct tttctatttg
     4501 ttttatatca tgctcactca aacaataatt agtattatca gtatttgttt tagaaagata
     4561 atccattata gcatctttaa attctaatat atccattttt gaatgtaaat gttcacttat
     4621 attggttaca cgagatttta cagattttat tccttttcct tcaaatttta taggtttaac
     4681 ctttaatgct tttgagaggt catttatttc agatgaaaat aaaagagtcc catgatgcat
     4741 tactttgttt ttgtagttgt attgagcatt tccagaaaat ttcttgccat ctataagaag
     4801 gtcatttctt ccagaaaatt cggcattgac atctagtgtt ttaagtaaat ctattatgag
     4861 ctgagtgaat cttctgaaat cactaaaatt attattgtta caagctataa atgtaaaatt
     4921 tatatttccc aaatcatgaa atacagctcc accaccagac tgtctcctaa cgacaggtat
     4981 agaatattct tttatatatt ctaaatttat ttcagaaagt gtgttttggt tttttccaac
     5041 aattatagaa ggttcattcc tccataaaat aaataagtct tcatcaaaat tttttagaag
     5101 atattcttcc attgctaaat taaaataagg gtttgttttt tcattataaa ttaataacat
     5161 taaagtagtt cctccataga attgataaaa tttttattat gaatacaatt aattttataa
     5221 ttcgacaata atataaaaac tcctttaaat tagaaatttt aaaaactacc ttgcaaagtt
     5281 taaataaata atatatactc aaaatatgtt taataaaagt gaatagaagg gtagaggcgc
     5341 aataatctat cagtactaat aaggagttaa gcacagagag ttattaggaa aggagatatt
     5401 gccgaagttt tataatcgat gctttaagat tatattgctg ggggtacata gaaaatatgt
     5461 acaactgtca caggtttatt tgtggtgagc tatcatttaa ggaattatat tatatttatt
     5521 tttactattg ttgatatatt gtaatgttta agccttgagt gtttaccact caaggctttt
     5581 ttagttttat tgtaacataa taccctgtac agttcacaaa attaaaagga gagggagatt
     5641 tatatgagga acaaaaaaat taatattatt tttctgacga caatcatgtt cataatgtca
     5701 acagtaatgg tttttgcaga agaagatata gacactattg cactagccaa tgcagaaaaa
     5761 tttgggattt taactttaat accaccatta gttgctataa tacttgcatt tataactaaa
     5821 aacgtaataa tttctttact tataggtatt ctttctggta gttttataat taaagctagt
     5881 ggtattaatg tttttgcgac atttatacag gcatttttag atttagtaga tagagcttta
     5941 gtatctttag cagacccttg gaatgcaggg ataatactac aggttttggc aattggagga
     6001 gtaataaatc ttgttgctaa aatgggagga gctaaagcca tagctgaagc acttgctaaa
     6061 agagcaaagt cagcaaaagg gacacagctt ataacatggt ttttggggct tttagtattc
     6121 tttgatgatt atgccaattc actaatagta ggtcctatga tgagacctgt tgcagataaa
     6181 atgaaaatat cgagagaaaa gctggcattt ataatagatg ccacagcagc accagttgct
     6241 ggtttagcta taatatctac atggataggt cttgaggttg gtttaataca tgatgctttt
     6301 gaaagtataa gtatagatgt agatgcattt ggtatatttt taaatacaat accatttaga
     6361 ttttacaata tattaatatt agctttcatt gtaatatcag cattattatt aaaagaattt
     6421 ggtccaatga gaaaagctga gataaaatct agaagtagaa aaattagtat agaccttgat
     6481 gaaggcgtag aagagttgga tgatttagct ccaaaaaatg gagtaaaatt gagtgtatgg
     6541 aatgctataa tcccaatagg aacattgata atagttgcat tagcatcatt ttattatagt
     6601 ggatatacat ctataatggg aggagacgat aaggctctga tacaactatt tacaaattct
     6661 ccatattctt ttgaagcaat aaaagaagca tttagcgcat ctgatgcatc tagagcatta
     6721 ttccagtctg cacttgttgc aagtttagtt gctattataa tggctgtagt aaaaaagatt
     6781 tttacaatat cagaagcaat tgatgtttgg atagatggaa tgaaaagttt agtaatcact
     6841 ggtgtaatat taatacttgc atggtcatta agttcagtaa taaaagagtt aggtacagct
     6901 aaatttttaa tacacctatt atcaggttca ctaccaccat tcttattacc aagtttaata
     6961 tttggtttag gagcaataat atcttttgct actggaacag cttatggaac aatgggtata
     7021 cttatgccac ttgcaattcc attagcatac tcattaaatc ctgatatgtc ttatgtaata
     7081 gtaagtacta gtgcagtttt aacaggtgca atatttggag accattgttc tccaatttca
     7141 gatacaacaa tactttcttc aatgggagct ggctgtaatc atatagacca tgttaacact
     7201 caaatgccat atgctatatt tacagcagta atcactattg tatttgggta tataccagct
     7261 ggacttggat tgccaatata tatagtatta ccagtggcaa tagctgctat atttgtaggt
     7321 attcaaataa ttggtaagaa agttgatgaa gcggaaatag aacttgtaga ataaagatat
     7381 aaataagttt caccttttat tatcactatt ttgttagttt ttagataatt ttaaaaagtt
     7441 attgaaaata atggcgaaat atagtaaaat aaaagagtga taagcggatg tggcggaatt
     7501 ggcagacgca ctagacttag gatctagcgc cattggcgtg ggggttcgac tcccttcatc
     7561 cgcactttac agtacttaat tgtactgaaa aaaactgggg ttgctggctt tcggagtggg
     7621 ctatcccgtt tttatttttt tgtatatata atagattggc actttagtat aaggtgcttt
     7681 ttttataggc aaaaacagta catttatttc tgttttatta tcattcatct attagaatat
     7741 actttagtta agaatgtgtt aataagcagt gttcacattc ttgataaaat gtggaataat
     7801 atgtattttt actaagacgg aaatgaaata attaaaccat taaataatat tataagatga
     7861 tgaataagta gataaatcaa attttgtagg aggatattat ggaaaaatat atatggttgt
     7921 ttccattact ttttattttt catgatatgg aagagataat aggttttgga atatggctta
     7981 aaaaaaataa atcaatgctt gataagaagt atccatttat cagtaaagtt tatgaaaatt
     8041 atagtactga aggcatggca tttgctgtgt ttgaagaatt tattttatgc attattttct
     8101 gtatacttac agttataaca gagaatcagt atgtatatct actatggtta ggtagtttta
     8161 tagcatatac tttacatttg gtaattcata tagggcagtc aatcataata agaaaatata
     8221 taccttcact tattaccagt atcatatgct taccaataag catttggtgt ataagtaaga
     8281 gtatttatat agttgattgt gaaatgagta caacaattct gtacagtata attggcatta
     8341 taattgtagc attgaattta aagtttgcac agtcgctgat tggaaaattt acaaagtgga
     8401 tgtcaaacat ataaatttta gtttattgga cagttcaagg aatcagaaat tgaaatttta
     8461 tagtattaat attttatagt attaatataa tgtataaaat tttatagcaa tgtatttcat
     8521 aagtataaag tgactagaat tttaagaaat atacaaattc tataaaaact aaataatttc
     8581 aagtgtattt taaaaatctt atttttcaca agtttaaaat tgtaaacgat ttcatcaagt
     8641 ctatgaatat acttttaaca tgttatagta aatctcaata taaatttaca tttggttctt
     8701 tgtgtgttat tttaataata aattaagtga aaatgttatt ttacattaat atataatatc
     8761 tttgcataag agtgaataat ataaaagaga aataattgat aataaaaact taacaaaaaa
     8821 attaatacat aaaaaaaaat aaatataatg tgttgacatt taattgaaat attcatacaa
     8881 taaaattgaa aataaatatt tccaaatgag attagcgaga gagtccttta tacgaaaagg
     8941 cgccgaagaa gaaatttaag aaatgccaat ttttagagaa gctttcaggc aaaaatatcg
     9001 ctaattgatg ggactctgga gagacttaag ttaagatact ataagtcacc gaagaagcaa
     9061 aataatatta aattattaaa tctttcaggt atacaaacag ggatatatag gctaggatta
     9121 ttttccatgc tgatatatct tttttttatg aaaaagtaca ggaaataata tcaaagttac
     9181 ttaaatacag taggtctaat tttctaggga gggttatata tgaatgaatt gaaaagagta
     9241 tctttgtaca atatacacaa agagcttgga gcaaaacttg ttgaatttgc tggttgggaa
     9301 atgcctttag aatatgaggg tataaataaa gagcatgaga aggtcagaaa aagtgcaggt
     9361 atatttgatg tatctcatat gggagaggta cagataaagg gagcagagtc agaaaaattt
     9421 atacaaaacc tagttacaaa tgatataagc acattaaaga taaatgacat tatatataca
     9481 cctatgtgtt atgaaaatgg tggagtagtt gatgatttat taatatataa atttggtgaa
     9541 gaagattatt tattagtaat aaatgcaggt aatatagata aagatgtagc atggataata
     9601 aaacaaagtg aaggatacaa tgtagatata aaaaatatat ctagtgaagt gagtcaatta
     9661 gcaattcaag gacctaaagc agaagaaatt cttcaaaaaa tcacagatat agacttaaac
     9721 tcaataaaat tttataagtc aattccatca acaaaagtat gtggttgtcc ttgtttagtt
     9781 tcaagaacag gatatactgg ggaagatggg tttgagatat actgtaaaaa taaatatgta
     9841 gaaataattt ggaatgaagt tctaaaggtt ggaggagaag atatatgtcc agctggtcta
     9901 ggttgtaggg atactttgag atttgaagca gctctacctc tatatggaca tgaaataaat
     9961 gaacacatat ctccaattga aggtggttta tctatctttg tgaaaacaaa taaagaatca
    10021 tttattggca aatcaatttt atcaaaagaa aaagaaagtg gagctaaaag aaagttagta
    10081 ggatttgaaa tgcaaggtaa aggaatgcct agaaatggat atgatataag gattggtgat
    10141 aaaacagttg gatttgtaac aacaggatgt gcatctccaa cgacaggaaa aattttaggt
    10201 atgggaatta tagattctga atatgcaaaa gtaggaaatg aaataggtat agcaatcaga
    10261 aaaaaagttg ttcctgctgt gatagttaag aaaccttttt ataaaaaaca atataaaaaa
    10321 gataatatta tattaaacaa agaaaataaa ttttcatata tacctgctac aagtgaagat
    10381 aaatcaaaaa tgttaaaagt agttggatta aattcggttg atgaattatt ttcagatata
    10441 ccagaagaag taaaattaaa aagagattta aatttggaaa ttggaaaatc tgaattagag
    10501 gtaagcaaaa tagtaaaaag attgtcagaa gaaaatttaa gtttagaaga cttgacttgt
    10561 tttcttggtg ctggtgcata tgaccattat ataccatcaa ttataaaaca tataacttca
    10621 agatctgaat tttatacagc atatactcca tatcaagctg aaataagtca gggaacttta
    10681 caagtagttt ttgaatttca atctatgatt gcagaaatta caggtatgga aattgcaaat
    10741 gcatcaatgt atgatggagc aactgctgca atagaagcat gtataatggc aatgaatcag
    10801 actagaaaaa gtaaaatagt agtgtcaaaa actattcatc ctgaaactct gtcaatatta
    10861 agaacttact tacaatataa agattgtgag atagtagaaa ttgacttttg taatgaatat
    10921 gggactacag atatagaaaa attaaaagct tctgtagaca aagatacagc atgtgttctt
    10981 atacaaactc ctaatttctt tggaattatt gaagaaatgg aagaaataga aaagataact
    11041 catgaaaata aggctatgtt aattatgagt gtagacccaa tatcattagg agttttaaaa
    11101 acaccaggtg agataggtgc agatattgtt gttggagaag ctcaatcact tggaaaccct
    11161 cttaattttg gaggtcctta tgtaggattt ttagctagca aatctaaata tactagaaaa
    11221 atgccaggaa gaatagttgg gcaaagttta gatgtagaag gtaaaattgc atatgtatta
    11281 actttacaaa ctagagaaca gcatgtaaga agagaaaaag caacttctaa tatatgttca
    11341 aatcaagctt taaatgctct tgtagcttca atatatatgg ctactatggg taaagaagga
    11401 tttaaagagg ttggtatgca gtctatgaaa aaagcacatt atacttacaa taagcttgta
    11461 caaactggaa aatataagcc aatatttaaa ggtaagtttt tcaaagaatt tgctgtacaa
    11521 ggaaatctta atatagaaac tataaatgat aaactcttag aagaaaatat attaggtggt
    11581 tataatttag aatacaatta tccagaatta aaaaattcaa ctttgctttg tgtgactgag
    11641 aaaagaagca aagaggaaat agataagtta gttggaataa tggagggatt ataatgaaag
    11701 aatacaatag tttgttgata gatatatcta aaaaaggtag gaaagcatac tcacttccaa
    11761 agttagatat agatgatata aaaatagaag atatgattga ccaagatatg gcaagaaaaa
    11821 gtgagttgaa tttgccagaa gttggtgaat tagaattagt tagacattat acattattat
    11881 caaataaaaa ctttggagtt gatacaggtt tttatccttt gggttcttgt actatgaaat
    11941 ataatcctaa aataaatgaa gatatggcag cacttccaaa ctttactgga atgcatccat
    12001 accaatcttc ggatacagct caaggttctc tttctttgat gtatgattta tcaagaagac
    12061 ttgcagaaat tacaggtatg gatgaagtta cactacaacc ttcagcaggt tctcatggtg
    12121 agtttacagg gcttatgatt ataaaagcat atcatgaaaa tagagatgac cataagagaa
    12181 ccaaagtgat aattccagat tctgcacatg gaacaaatcc agcaagtgca gctatggcaa
    12241 actttgatgt gattcaaata gcatctgata aaaatggagc tgtagacata aatgcattaa
    12301 aagaggtttt gaatgatgaa gtagcagctc ttatgcttac taatccaagt actcttggtt
    12361 tatttgaaaa aaatataaaa gaaatagcta ctcttgtaca tgaagcagga ggacttttgt
    12421 actatgatgg tgcaaacatg aacgctatta tggggataac aagacctgga gatatggggt
    12481 ttgatgtggt tcatcttaat cttcataaga ctttttcaac tcctcatgga ggtggaggtc
    12541 caggagctgg tcctgttgga gttaaaaaag aattagtacc ttttttacca ataccagtaa
    12601 ttgaaagaaa agaagataag tatgtactaa attatgatag agaaaaatct attgggaaaa
    12661 ttaaaaattt ctatggtaat tttggagttc ttgtaagagc ttacacatat atactaacta
    12721 tgggaaggga tggacttaaa gaggctagtg aaatggcagt tttaaatgct aattatataa
    12781 aggaaagtat aaaagatgat tatattttgc caatagatac cttgtgtaag catgagtttg
    12841 tattaggtgg attgccaaga ggagaggcca atataaaaac tatcgatatt gcaaaaagac
    12901 tactagatta tggataccat cctccaacaa tgtactttcc tcttattata aatgaggcac
    12961 ttatgataga acctacagaa agtgagagta tagagacatt agatagtttt atagaagcta
    13021 tgaaaagtgt tgctaaagag gcaaaggaag aaccagaact attgaaaact gctccacata
    13081 atactttggt caaaagagta gatgatgcaa gggcagtaaa gaaacctata ctaacatggt
    13141 cacaaagata aatagttaaa aagtagagat ttaaagtata aagagaatta aatttgtaag
    13201 aaaaagctat ctaacttaat ctagtttaga tagctttttt gtatattagt atgtatggta
    13261 ttttctatat aaagttttaa attgtattag tgcttatata gaatattgat atattatgta
    13321 tagatattaa taagaattgt tgaatttatc ttgccttaac tttatttact cttttatatt
    13381 aagaaaaact taactaaaat atagcgaaat tcataaaaat ttaggaaatc ttaagaatta
    13441 taata
//
LOCUS       DBGJFF010000103        13398 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.104, whole genome shotgun sequence.
ACCESSION   DBGJFF010000103 DBGJFF010000000
VERSION     DBGJFF010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13398
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.104"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            163..1239
                     /locus_tag="ACOIAF_002865"
     CDS             163..1239
                     /locus_tag="ACOIAF_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HGZ2669867.1"
                     /translation="MKAQRLNAVLEQMKKDDISQMLVSDPTSIFYLTGVLIHPGERLL
                     ALYLNLNGNNKLFINELFPVSEDLGVEMVWFNDTQNPVEIITEHIDKNATMGVDKNWP
                     ARFLLNLIELGGGSKFVNSSYIIDTLRMCKDEEEKELMRIASKLNDKAMEQLKATVSG
                     ELTEKQLVGKLSKIYEDLGTDGFSFDPIIGFGPNGANPHGEPGNALVKPGDAIILDIG
                     CIKDNYCADMTRTVFYKEIPEKGREIFEIVLEANKRAEAIVKPGVRFCDIDAAARDYI
                     TEKGYGQYFTHRTGHSIGLEVHDKGDVSSINTDTVQPGMIFSIEPGIYLPGEFGVRIE
                     DLVLVTEDGCEILNKHDKEICVVG"
     gene            complement(1356..2198)
                     /locus_tag="ACOIAF_002866"
     CDS             complement(1356..2198)
                     /locus_tag="ACOIAF_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HGZ2669868.1"
                     /translation="MDKIKLIVDSACDLPDDIIEKYNIEVVGLNVSFGEESYISGKEI
                     DNETFYRKMSESKVLPKTSCPSPDKFLEAYHCQEESVLVINISSGLSGTYNSALLAKD
                     MFEKEGNNKKVEVIDSLSGSIGIGQLVLKAAILIKEGKEIEEIVSIINKYKDNPPFFG
                     TLETLENAIKGGRINPIAGKIINTLNFKAIIQVADGVVTPIDKARGEGNSLKKLITLV
                     ESKIKDKEEKVLFIGHANCPEKAQKVREVMEKNVKYKDVVICEIGSVMGTYTSKGAIL
                     ITAI"
     gene            2382..2954
                     /locus_tag="ACOIAF_002867"
     CDS             2382..2954
                     /locus_tag="ACOIAF_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HGZ2669869.1"
                     /translation="MKTNNMKQAILSSATNLIAKNGVQNTSLADIAKDVNISKGTLYY
                     HYASKDDIIYDIADTHLEVITSAILNCVKNVKSKNSQIEMVNLILEKISTIESRGRVH
                     MYLICEAITSNNDLKERIRLKYIEWRTTLKSEIVESLEKYNSSENEQDAESFSFLLMS
                     IVDGLVVQSLLKTEKIPYENIASFLVNHWI"
     gene            complement(3117..3389)
                     /locus_tag="ACOIAF_002868"
     CDS             complement(3117..3389)
                     /locus_tag="ACOIAF_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669870.1"
                     /translation="MLRERFVVRNHHNPHTSGCCGTGGEGCCGGDNHEHSHQHSHSHG
                     HEHEHVNGVGHDHNHDDGFSGTHEHSHEHTHTHGHEHSDGHTHEHK"
     gene            complement(3503..4375)
                     /locus_tag="ACOIAF_002869"
     CDS             complement(3503..4375)
                     /locus_tag="ACOIAF_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HGZ2669871.1"
                     /translation="MYGESKKLSIDNNIFLQYCLIILGMILSTLGINLYLAPAKLLSG
                     GVAGICVILYKLFGINQGISSFLMNIPIFIIARKYFDNKFLLISFVNMLLFSLALGLT
                     QDIAKYFPIDDTMLQCIYGGALTGIGMGLTFKARATAGGLDIIAAIMKRKYDIPMKNT
                     FLFINFFVVCAGAFLFGAKLVMYTLITMYIISFTMDIGKDCFDRKKSILLISNKYDEI
                     SKVIMNKMGRGVTFLEAEGAYTQNKKKMIYCIISANEIGKFKDLIYDMDSEAFISVNN
                     VEEVKGGGFKDKFL"
     gene            complement(4671..7778)
                     /gene="ileS"
                     /locus_tag="ACOIAF_002870"
     CDS             complement(4671..7778)
                     /gene="ileS"
                     /locus_tag="ACOIAF_002870"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HGZ2669872.1"
                     /translation="MAKFKPLVDSSVKQAEAQVFDYWKDINILEKTLEKGKDDPSFVF
                     YEGPPTANGNPGVHHVLSRTLKDSVCRYKTMSGYQVKRKAGWDTHGLPVEIQVEKELG
                     LTSKQQIEEYGIAEFNQKCRESVFSFEKQWRIMTERMAYEVDLDNPYITLDNNYIESV
                     WWILNKFNKEGYIYEGHKILPYCPRCGTGLASHEVAQGYKEVKTNTVIAKFKKKDADE
                     YFLAWTTTPWTLPSNVSLTVNADVDYLKVKKGDEVYYVSKPLADKVLGEDYEVLEEMK
                     GKDLEGLEYEQLMPFVEVDKKAFFITVGDYVTTEDGTGIVHTAPAFGEDDYNTGKRYD
                     LPVIQPVDETGKFTSTPWEGRFVMEDGLDVEIIKWLASENKLYSKEKVVHNYPHCWRC
                     QTPLVYYAKPSWYIEMTKLKDKLIENNNGVKWFPSFVGEKRFGNWLENLNDWAISRTR
                     YWGTPLPIWRCECGHTDSVGSRAELAEKAIEDVNPETVELHRPYVDDIHLKCEKCGKP
                     MTRVTEVIDCWFDSGAMPFAQHHYPFENKENFDQLFPADYISEGIDQTRGWFYSLLAV
                     STFVLGKAPYKSVLVPDLVLDKDGKKMSKSRGNTVNPMELFDQYGADALRWYLLYVSP
                     PWTPTKFDMDGLKEIQSKFIGTMKNVYNFFTLYANTDDINPTEFFVEYKDRPELDRWI
                     LSKFNNLMKDVEENLAIFELNKTLRMIQDFINDDLSNWYIRRSRRRFWATELTEDKKA
                     VYNTTYELLHGLCRAIAPFAPYMSEEMYRNLTGEVSVHLAEYPKCNEELVDTKLEEKM
                     DLAKNLVTLGRASREVERIKVRQPLQKVLVDGKFEDTISDVVDLIKEELNVKEVIFAK
                     DLDEYMNFSLKPNFKEAGPLLGSKMNLFVGALSKLNAHETANKLEKGETLTVDLDGEA
                     FEFNKDLVLIGITAKEGFNVSVENNLFVILDTKLTEELINEGYAREFISKVQQLRKSN
                     NFEVLDNIVIEYCGDDEIAKAVDHFNEYIKSETLALEINRVDDKSLEEQNLNDHMTGI
                     KVIKK"
     misc_binding    complement(7871..8103)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8278..8805)
                     /locus_tag="ACOIAF_002871"
     CDS             complement(8278..8805)
                     /locus_tag="ACOIAF_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HGZ2669873.1"
                     /translation="MLTPIEIENKEFKKGLRGYRDEEVDEFLDIIKEDYESLCRENTA
                     LKEKLGLYQDQVNKYENIEETLKATLITAQSAAEDTCSAANKKAKIIVEEADLKARQI
                     IEQANNRVIEIRKEYDALVKEFKIFRNKFKSLLEDEIRSVDEIFYDVDEKCVNAFEGT
                     TVYNYSNDEAATTLE"
     gene            complement(8840..9625)
                     /locus_tag="ACOIAF_002872"
     CDS             complement(8840..9625)
                     /locus_tag="ACOIAF_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmH/Sll1252 family protein"
                     /protein_id="HGZ2669874.1"
                     /translation="MDKLKLTNHIKDIDLKNKMFKVIDKANSCIKNYDVKSTEFLNPY
                     EVKNAVAILNSTNEIKYSVDGGYEQAERSTVFIYPFYMEYEDIEDTLRFLQIEGNFKF
                     KNISHKDYLGSILGLGIKREKIGDIIIHDSFCQVVVSSDICDFIIVNLEKVSRNNVIV
                     KEISRENIVNSSSKYKEVSFTVSSDRLDCVISGIYNISRQDSAKYINGEKVHVNYEKI
                     TSTSKIVKNDDLISIRGKGRAKVTQIGDITKKGKIKVQARLIV"
     gene            complement(9635..9892)
                     /locus_tag="ACOIAF_002873"
     CDS             complement(9635..9892)
                     /locus_tag="ACOIAF_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HGZ2669875.1"
                     /translation="MGTIRIALYYLLDIIAWMIVIKSLMTWFPNGRQSKIFDILENLT
                     EPIEGPIRSIMYKYTNGPIDFSPLIAIVLLMFLRQLVLIIF"
     gene            complement(9911..10369)
                     /locus_tag="ACOIAF_002874"
     CDS             complement(9911..10369)
                     /locus_tag="ACOIAF_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HGZ2669876.1"
                     /translation="MSNGIISKFKNWIVDEEEDYIEDEYESGMDDIVQEEEMNSGFST
                     AKANKIVNLHTTSQMKVVIVEPKVYDEAATIADHLKQRRAVIVNLEGLTNSEVRKSIF
                     NFMNGAVYVLDGSIQKVSKSIFILAPNNVDIDANMKKELESKAFFPWQNK"
     gene            complement(10409..11113)
                     /locus_tag="ACOIAF_002875"
     CDS             complement(10409..11113)
                     /locus_tag="ACOIAF_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HGZ2669877.1"
                     /translation="MGSIKDNLSEIRNKIDKAAKKVDRDSKEITLLAVTKTVDVDLVN
                     EAMREGITSVGENKPQELARKYDIIGDKLNWHLIGTLQTNKVKYIIDKVCMIHSLDRI
                     ALCEEIQKRAEQINREIDCLVQVNISKEESKHGLDREEVVDFVKEVSEKYKNIKIKGL
                     MTMAPFIEDEDEIRGVFKGLKDLSKQIKELNIPNVGMDVLSMGMSHDYEIAIEEGATI
                     VRVGTSIFGERNYNKK"
     gene            complement(11123..11758)
                     /locus_tag="ACOIAF_002876"
     CDS             complement(11123..11758)
                     /locus_tag="ACOIAF_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HGZ2669878.1"
                     /translation="MKKVKYTRLVILGILIYLVFNILVMIISKNVDTLVLKNEKVDAK
                     IHVKGTIEKDANDQSNYLSTYISKEDSNNFEKNQNVIIEYHEQKLDAKVYKIYENNNK
                     IMVKLKISNQIVANQDTSVEEFDIIYNQMECLKIPKTSIKTKDNKRGVYVIDEQSQSV
                     KFVILEGITYENESSVFVDYYKNDINGVTSVNLYDKIILRPNSINTNIRIK"
     gene            12139..13179
                     /locus_tag="ACOIAF_002877"
     CDS             12139..13179
                     /locus_tag="ACOIAF_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HGZ2669879.1"
                     /translation="MSEILLGTGLLLLVLCCFTIFSYKAPYGMKAMGALANAACATFL
                     VEAFNLSLLGDVLGMEFFRSVGAANGSLGGVAAAILVPLALGVSPVYAVLIGLSCLDF
                     SILSGFVAGYFVSFLIKFIEKKVPPGVDLVVVILLCAPLSRLIASNVDPLVNSTLLQI
                     GQVLIEATNISPIIMGIILGGLITVVGTSPLSSMALTSIIGLTGLPMAIGALGAFGSA
                     FMNSTLFAKLKLGSKKDIISVAIEPLTQSDIISANPIPIYSTNFFGGAFSGIIVALMG
                     LVNMTPGTATPVAGFAIMFAYNPVQQVLITAGLCALFNVIAGFLGSKIFSTFKVLTAG
                     EIRGTDVEEEIA"
BASE COUNT         4266 a         2259 c         1584 g         5289 t
ORIGIN      
        1 aaagtcattt tacttccgta tttaatgcaa aagtagagat taaacttaat ataaaaaata
       61 tataacaaat tttttttata tttctagaag aaaagtatac tcttttttgt ttaataatgc
      121 taaaattgat ttgtattgaa ttttgattag gaggaaatta ttatgaaagc tcaaagatta
      181 aatgctgtac ttgaacaaat gaaaaaagat gatatttctc aaatgttagt aagtgaccct
      241 acttctatat tttatcttac aggagtacta atccatccag gcgaaagact attagcatta
      301 tacttaaatt taaatggcaa taataaatta tttataaatg aattattccc agtttcagaa
      361 gatttaggtg ttgaaatggt ttggtttaat gatactcaaa atccagtaga aataataact
      421 gaacatatag acaaaaatgc tactatgggt gttgataaaa actggccagc tagattctta
      481 ttaaacttaa tagaattagg tggaggaagt aagtttgtaa attcatctta tataatagat
      541 actttaagaa tgtgtaaaga tgaagaagaa aaagagttaa tgagaatagc ttcaaaatta
      601 aatgacaagg ctatggaaca acttaaagct actgtttctg gtgaacttac agaaaaacaa
      661 cttgttggta aattatctaa aatatatgaa gacctaggta ctgatggatt ttcatttgac
      721 cctattatag gttttggacc taatggagct aatccacatg gtgaacctgg aaatgcactt
      781 gtgaaaccag gagatgcaat aatcttagat ataggttgta taaaagataa ctattgtgct
      841 gatatgacaa gaactgtatt ctataaagaa atacctgaaa aaggtagaga aatatttgaa
      901 atagttcttg aagctaataa gagagctgaa gctattgtaa aaccaggtgt tagattctgt
      961 gatatagatg ctgctgctcg tgactacata actgaaaagg gatatggaca gtacttcact
     1021 catagaactg gtcactctat aggacttgaa gttcatgata aaggagatgt atcttctata
     1081 aatacagata ctgtacaacc aggaatgata ttctctatag agccaggtat atatttacca
     1141 ggtgagttcg gagttagaat agaagattta gtacttgtta ctgaagatgg ttgtgagata
     1201 ttaaacaagc atgacaaaga aatatgtgta gttggttaat tctaaaatca atttgataaa
     1261 attaaaaagc catataaaaa tgttttaaac atttttatat ggctttaatt tctcaatcta
     1321 ttatttattt caatccatta tttatattgc agtatttata ttgcagttat taagattgca
     1381 ccctttgaag tatatgtccc catcactgaa ccaatttcac agattacaac atctttgtat
     1441 tttacattct tttccataac ttcccttacc ttttgagcct tttctgggca attagcatgt
     1501 cctataaata aaaccttttc ttctttatct ttaatttttg actctactaa agtaattagc
     1561 tttttcaaac tgtttccttc acctcttgcc ttatctatag gagttacaac gccatctgca
     1621 acttgtatta tcgccttaaa atttaaggta tttataattt ttcctgctat tggatttatt
     1681 ctaccaccct taatagcatt ttctaaagtt tcaagtgttc caaaaaatgg aggattatct
     1741 ttatatttat ttattattga aacaatctct tcaatttctt ttccttcttt tattaatatt
     1801 gctgctttta acactaattg tcctattcca attgacccac taagtgaatc tataacctca
     1861 actttcttgt tatttccttc cttttcaaac atatcttttg ctaaaagtgc actattataa
     1921 gtcccagata atcctgaact tatattaatc actaatacac tttcttcctg acaatggtat
     1981 gcttctaaaa atttatcagg agaaggacat gatgtttttg gtagtacttt actctcactc
     2041 atttttctat agaaagtttc attatctatt tcctttccag atatataact ttcttctcca
     2101 aaagatacat ttaaaccaac aacttctatg ttgtattttt ctattatatc atcaggtaag
     2161 tcacatgcac tgtccactat caattttatt ttatccatat taacctcact ataagatatt
     2221 attttattta ttcagtcggt cgtacgaccg atttaattat tattttatat attgaatgaa
     2281 aaaaagtcaa tagatataga ttctcgcttt attgacaata aatatccata agcttataat
     2341 gttattaatt tgttataaat atacatggaa aggtaggttt tatgaaaaca aataatatga
     2401 aacaggctat tttatcatct gcaaccaatt taatagccaa aaatggagtt caaaatacta
     2461 gccttgctga tattgcaaaa gatgtaaata taagtaaagg tactttgtat tatcactacg
     2521 catctaaaga tgatattata tatgatattg cggatactca tttagaagtt ataactagtg
     2581 ctatactaaa ctgtgtaaaa aatgtcaaat caaaaaattc tcaaatagaa atggttaact
     2641 taatcttaga gaaaatatct actatagaaa gtcgtggtag ggtacatatg tatcttattt
     2701 gtgaggcaat tacttcaaat aatgatttaa aggaaagaat aagattaaaa tatattgaat
     2761 ggagaactac tcttaaatct gaaattgttg aatcactaga aaaatataat agtagcgaaa
     2821 atgaacagga tgctgaatca ttttcttttt tactcatgtc aatagttgat ggtttagttg
     2881 tccaaagttt attaaaaaca gaaaaaatac cttatgaaaa tatagcttct tttttagtta
     2941 atcattggat ttaaacttaa tacttagata attaataaaa gtgaactatt taagttatct
     3001 actttaacag ttcactttct tatttattct caataatttt tacttaaaac attcaatttt
     3061 tatttaaacc ttcatggaat cacaaaataa aaatctctta taaatttaaa atgtttttat
     3121 ttatgttcat gtgtatgccc atctgaatgt tcatgaccat gtgtatgtgt atgctcatgg
     3181 ctatgctcat gagtacctga aaaaccatca tcatgattat ggtcgtgacc tacaccattt
     3241 acatgctcat gttcatgccc atgactgtga gaatgttgat gtgaatgttc atggttatct
     3301 cctccacagc atccttctcc tccagtccca caacacccac ttgtatgtgg attatgatga
     3361 tttcttacta caaatctctc tcttaacata attcttatac ctcctagtat attattgagt
     3421 acttcatgta ccccattaac tacagtatac atctaactag gtataatctc aatacatatg
     3481 gcatttttta acatatataa tcttaaagaa atttatcctt aaaacctcca ccttttactt
     3541 cttcaacgtt attgacagaa ataaaagctt cactatccat atcataaatt aaatctttaa
     3601 atttacctat ttcatttgca gatattatac aataaatcat ctttttctta ttttgtgtat
     3661 atgcaccttc tgcctccaaa aaagtgactc ctctacccat tttattcatt atcactttgg
     3721 atatttcatc atatttattt gatattaaaa gtatagattt ttttctatca aagcaatctt
     3781 ttcctatatc catagtaaaa gaaattatat acattgtaat taaagtgtac atcaccaatt
     3841 tagctccaaa taaaaaagct cctgcacata ctacaaaaaa attaataaat aaaaatgtat
     3901 ttttcatagg tatatcatac tttcttttca ttatggctgc tattatatca agtcctccag
     3961 cagtagctct tgctttaaaa gttaacccca tacctatacc agttaatgca ccaccataaa
     4021 tacactgtag catagtatca tctattggga aatatttagc aatatcttgt gttagcccca
     4081 gtgctagaga aaatagtaac atattgacaa aacttattag caaaaattta ttatcaaaat
     4141 actttctagc tataataaat attggtatgt tcatcaagaa acttgatatt ccctgattta
     4201 taccaaataa tttgtataat ataacacaaa ttccagctac tcctccactt aataacttgg
     4261 ctggtgctag atataaattt attcctaagg ttgatagaat catacccaat ataataagac
     4321 aatattgtaa aaatatatta ttatcaatac ttagcttttt actttcacca tacatcaaaa
     4381 cgcacccctt tgcataataa ataaaccacc ctgcataaca cacgataaat ttacaaataa
     4441 atttatcaga agtagtttcc taaaaatatt attcatcctc acttaaacgt ttattcatta
     4501 atattctatc acatgacata aataatttat atgatttata tagatttatt tattgtaaat
     4561 tttggaatat taaaacattt tattgaatag ataattatat tacataaaaa atccctatag
     4621 aaatctataa actaatttct atagggattt tggttaacta actattattt ttattttttt
     4681 ataactttta tacctgtcat atggtcattt aagttttgtt cctctaaaga tttatcatct
     4741 actctgttta tttctaaagc taaagtttca gacttgatat attcattaaa atggtcaaca
     4801 gccttagcaa tttcatcatc accacagtat tctattacta tattatctaa tacttcaaag
     4861 ttattagatt ttcttaattg ctgtacttta gatataaatt ctctagcata accttcatta
     4921 atcaactctt cagttagctt agtatctagt ataacaaaaa gattgttttc gacagataca
     4981 ttaaatcctt ccttagcagt tatacctatt aatactaaat ccttattaaa ttcaaaagct
     5041 tcaccatcta aatcaactgt taaagtttca cctttttcaa gtttattagc agtttcatga
     5101 gcatttaatt tacttaatgc tccaacgaat aaattcatct tagaacctaa taatggccct
     5161 gcttccttaa agtttggttt taaactaaag ttcatatact catctaaatc cttagcaaat
     5221 attacctctt taacattaag ctcttctttt attaaatcaa ctacatcact tattgtatct
     5281 tcaaacttac catctactaa cactttttgt aatggttgac gaacttttat tctttctact
     5341 tctcttgaag ctctacctaa agtaactaag tttttagcta aatccatttt ttcttctaat
     5401 ttagtatcta ctaactcttc attacattta ggatattcag ctaaatgaac agatacttct
     5461 cctgttaagt ttctatacat ttcttctgac atatatggtg caaatggagc tatagctcta
     5521 caaagaccat gtaacaattc ataagttgta ttgtacactg cttttttatc ttctgttaat
     5581 tcagttgccc agaatcttct tcttgaacgt cttatatacc agttagataa atcatcattt
     5641 ataaaatctt gtatcattct taaagtttta tttaactcaa atatagctaa attttcttct
     5701 acatctttca ttaaattatt gaacttagat aatatccatc tatcaagttc tggtctatct
     5761 ttatattcta caaaaaattc agttggattt atatcatctg tgtttgcata taaagtaaag
     5821 aagttatata cattcttcat agtacctata aatttacttt ggatttcttt taatccatcc
     5881 atatcaaatt tagttggagt ccatggtgga gaaacatata gtaaatacca tcttaatgca
     5941 tctgctccat attggtcaaa taattccatt ggatttactg tatttcctct tgatttactc
     6001 attttcttac catctttatc aagtacaagg tctggaacta aaacactctt atatggtgct
     6061 tttcctaata caaatgttga tactgcaagt aatgagtaga accaacctct agtctggtct
     6121 ataccttctg atatatagtc agctgggaac aattggtcaa agttttcctt attttcaaat
     6181 ggatagtggt gttgagcaaa tggcatagcc ccactatcaa accaacagtc tataacctct
     6241 gttactcttg tcattggttt tccacatttt tcacatttta agtgtatatc atctacatat
     6301 ggtctatgaa gttcaactgt ttctggattt acatcttcta tagctttttc agctaattca
     6361 gctcttgaac caactgaatc tgtatgacca cattcacatc tccatatagg aagtggagtt
     6421 ccccaatatc tagttcttga tattgcccag tcatttagat tttctaacca gtttccaaat
     6481 ctcttctctc ctacaaaact tgggaaccat tttacaccat tattattttc tattaattta
     6541 tctttaagtt tagtcatctc tatataccag cttggttttg catagtatac taatggagtt
     6601 tgacatctcc agcagtgtgg ataattatga actacttttt ctttgctata taatttattt
     6661 tcagaagcta gccattttat tatttcaaca tctagcccat cttccattac aaatctacct
     6721 tcccaaggag tagatgtaaa tttaccagtt tcatctactg gttgaataac tggtaaatca
     6781 tatcttttac ctgtgttgta gtcatcttca ccaaatgctg gtgctgtatg aactatacca
     6841 gttccatctt cagtagttac ataatctcct actgtaataa aaaatgcttt tttgtcaact
     6901 tctacaaaag gcattaattg ttcatattct aaaccttcta ggtctttacc tttcatttct
     6961 tctaaaactt cataatcttc acctagtact ttatcagcta aaggtttaga tacataatat
     7021 acctcatcac cttttttaac ttttaaataa tctacatcag catttactgt taatgaaaca
     7081 tttgatggta aagtccatgg agttgttgtc catgctaaga agtattcatc agcatctttt
     7141 ttcttaaatt tagctataac tgtattagtt tttacttctt tatatccttg tgcaacctca
     7201 tgactagcaa gaccagttcc acatcttggg caataaggaa gtattttatg tccttcatat
     7261 atatatcctt ctttgttgaa tttgtttagt atccaccata cagactctat atagttatta
     7321 tctaaagtta tatatggatt atctaaatca acttcataag ccattctctc agtcattatt
     7381 ctccattgtt tttcaaatga gaaaactgac tctctacatt tttggttaaa ttcagctatt
     7441 ccatattctt ctatttgttg cttactagta agtcctaact ctttctctac ttgtatttca
     7501 actggtaatc catgtgtatc ccaaccagct tttctcttta cttgataacc agacattgtt
     7561 ttatatctac aaactgaatc tttaagcgtt ctagataaaa catggtgtac tccaggattt
     7621 ccattagctg ttggaggtcc ttcatagaat acgaaacttg ggtcatcttt ccctttttct
     7681 aaagtttttt caagtatatt tatgtctttc caataatcaa aaacttgtgc ttctgcttgt
     7741 tttacagaac tgtctaccaa tggcttaaat ttcgccatgt aaatcaccct ttcctactaa
     7801 atattataat tccaaaattt tttattcttt cacataaatt taaatactaa aaaactcgcc
     7861 ccctttaaac caaagggacg agttataaat cgcgttacca ccctaattct atatgtaaac
     7921 taaatactag ctacatataa cactctaata gtataacggt actaaccggc acagcttagt
     7981 agaatttaat cgttcagcct gcagctcaag agtgattttt atctttataa attcattggt
     8041 tctcagcata cccaattctc tgtaaaatta tctaaagata aatgtctcta tcaacgcttt
     8101 taaaaattta tctgtgttta tatgaaattc tataacaaat aatttgaata gtcaatatat
     8161 ttttgaaaaa tataataatt tctatttttc ttacattttt atcctatttt taatctttgt
     8221 tatagatttc tcaaactcac acaattattc tacataaaaa ataaacaaat tacaagctta
     8281 ttctaaagtt gtagcagctt catcattact ataattatac actgttgttc cttcaaatgc
     8341 gtttacacat ttttcatcta catcataaaa aatctcatca acacttctta tttcatcttc
     8401 taacagtgat ttaaatttat ttctaaatat cttaaattct ttgactaaag catcatactc
     8461 ttttcttatc tctataacac ggttattagc ttgttctata atttgtctag cttttaaatc
     8521 agcttcttca acaattatct tagccttttt attagccgcg ctacaagtgt cttctgctgc
     8581 actttgagca gttattaaag tagcttttag tgtttcttca atattttcat acttgtttac
     8641 ttggtcttga tataacccta atttttcttt aagagctgta ttttccctac atagagattc
     8701 ataatcttcc ttgatgatat ctaaaaattc atcaacctct tcatctctat accctcttaa
     8761 acctttttta aactctttat tttctatctc aattggagtt agcattacat ttcctcccag
     8821 ctataattta aattagtcct tatacaatta atcttgcttg aacttttatt tttccttttt
     8881 tagtaatatc tcctatttgt gttaccttag ctcttccttt gcctctaata gaaatcaaat
     8941 catcattttt tactatttta gaagttgaag ttatcttttc ataattgaca tggacttttt
     9001 ctccatttat gtattttgca ctatcttgtc tagatatatt gtaaatacca cttatcacac
     9061 aatctaacct atctgaagat actgtaaatg atacttcttt atattttgaa gaactattaa
     9121 caatattttc tctggaaatt tcctttacaa ttacattatt tcttgaaacc ttttctaagt
     9181 ttacaattat aaaatcgcaa atatcactac ttacaactac ttgacaaaag ctatcatgta
     9241 taattatatc tccaattttt tctcttttta tacctagacc cagtattgac cctaagtaat
     9301 ctttatgaga tatattttta aatttgaaat tcccttctat ttgtaaaaat ctaagagtat
     9361 cttctatatc ttcatattcc atgtaaaatg gataaataaa tacagtgctt ctttcagctt
     9421 gttcataacc tccatccaca ctgtatttta tttcatttgt agaatttaat attgctacag
     9481 catttttcac ttcatatgga ttaagaaact ccgtagactt aacatcatag ttttttatac
     9541 aactattagc cttgtctatc actttaaaca ttttattttt caagtctata tcctttatgt
     9601 gatttgttag ttttaattta tccatcctat caccttagaa tataatcaat actaattgtc
     9661 ttaggaacat caataatact attgcaatta aaggagaaaa atctataggt ccatttgtat
     9721 atttatacat tatagacctt attggccctt ctattggctc tgtaagattt tccaaaatat
     9781 caaaaatttt actttgtctt ccatttggaa accaagtcat caagcttttt ataactatca
     9841 tccatgctat tatatctaaa agataataaa gtgcaattct tattgttccc atatattaac
     9901 cgccttttta ctatttattt tgccaaggga agaatgcttt actttctaat tcttttttca
     9961 tatttgcatc tatatccaca ttattaggag ctagtataaa tattgattta gaaacctttt
    10021 gtatacttcc atctaagaca taaactgccc cattcataaa gttaaatata gatttacgaa
    10081 cttcagaatt agtcaatcct tccaaattca ctataactgc ccttctttgt tttagatggt
    10141 ctgctatagt tgcagcttca tcataaacct taggttcaac tattacaact ttcatttggc
    10201 tagttgtatg taaatttaca attttatttg cttttgcagt actaaagcca ctgttcattt
    10261 cttcttcttg aactatatcg tccatgccac tttcatattc atcttcaatg taatcttctt
    10321 cttcatcaac tatccaattc ttaaatttag atattattcc atttgacatt attaacttcc
    10381 tccttaaaat ttattcaaat ttgagtaatt attttttatt gtagtttctt tctccaaaga
    10441 ttgatgttcc aactctaaca atggtagccc cttcctctat agcaatttca taatcgtgac
    10501 tcattcccat tgacaaaaca tccatcccta cattagggat gtttagttct tttatttgtt
    10561 ttgataaatc tttaagacct ttaaatacac ctcttatctc atcttcatct tctataaaag
    10621 gtgccattgt cattagaccc tttattttta tatttttata tttttctgac acttctttga
    10681 caaagtctac aacttcttct ctatcaagtc catgcttaga ctcttcttta gatatattca
    10741 cttgtactaa gcaatctatt tctctgttta tttgttctgc acgtttttga atttcttcgc
    10801 ataatgcaat tctatctaaa gagtgtatca tacaaacttt atcgattatg tacttaactt
    10861 tattggtttg caaagttcct atcaggtgcc agtttaattt gtcacctata atatcatact
    10921 ttctagctaa ttcttggggt ttattttcac caacagaagt gattccttcc ctcattgcct
    10981 cattaactaa gtctacatca acagtctttg tgactgccaa aagggtaatc tctttgctgt
    11041 ctctatctac tttttttgca gctttatcaa ttttgtttct tatctcagac aaattatctt
    11101 taatagaacc cattattatc acctacttta tccttatatt tgtgtttata ctgttaggtc
    11161 ttaaaattat tttgtcatat agattaacac ttgtaactcc atttatgtca tttttataat
    11221 aatccacaaa tacagaagat tcattctcat aagttattcc ttctaatatg acaaatttta
    11281 cactttgtga ttgttcatca attacatata caccacgttt attgtctttt gtttttatag
    11341 aagttttagg tatttttaaa cattccatct gattatatat tatatcaaat tcttcgacac
    11401 ttgtatcctg atttgcaaca atttggttac ttatttttaa ttttaccatt attttattat
    11461 tattttcata tattttgtaa acttttgcat ctaatttttg ctcatgatat tcaattatta
    11521 cattttgatt tttttcaaag ttattagaat cttctttgct gatgtatgta gatagataat
    11581 tactctggtc attggcatct ttttctatag ttccttttac atgaatcttt gcatcaactt
    11641 tttcattttt taacaccaac gtatctacat ttttgctaat tatcattact aatatattaa
    11701 atactaaata tataagaata ccaagtatca ctaatctggt atattttacc tttttcaaag
    11761 actctccccc tataaaatta ctttcattat aagtacttct tctaaaaaat gtttattcct
    11821 tttttataag ctaatatttt acgttctaat aattattttt tccatattaa atttattttt
    11881 tatcaatctt cctatacaag actgcattat ttatatataa ttgtattata aagtataagt
    11941 ttaatttaca ttttatacgt taagcttact ttatatgttt ttatagttat ttttattttt
    12001 agatgcaata taaaaccaat taagatttaa aataactttt gttatgttga catttattta
    12061 ggtaatcatt tatgaatcag gataacgtta tcataacaaa agtttatgtc ctaaaaaaaa
    12121 tacaaggagg attttcttat gtcagaaatt ttattaggaa caggtttact tttattggtt
    12181 ctatgttgtt tcacaatttt tagttacaag gccccatatg gtatgaaggc tatgggagct
    12241 ctagcaaacg ctgcatgtgc tactttctta gtagaggcat tcaacctttc tttacttggt
    12301 gatgttttag gtatggaatt ttttagaagt gttggagctg ctaatggtag tcttggtgga
    12361 gttgctgctg ctatacttgt gcctttagcc ttaggtgtat ctccagttta tgcagtttta
    12421 ataggattat cttgtttaga tttttctata ttatctggat tcgttgctgg atactttgtt
    12481 tctttcttaa ttaagtttat agagaaaaaa gtgccacctg gagtggattt agtggttgta
    12541 attcttctat gtgcaccact atctcgtctt atagcatcaa atgtagaccc attagttaat
    12601 agtacactac tacaaattgg acaagttttg atagaagcta ccaacattag ccctataata
    12661 atgggaatta tactaggagg tctaataaca gtagttggaa cgtctccact tagttcaatg
    12721 gctcttacat ctataattgg tcttactggt cttcctatgg caataggagc attaggtgct
    12781 tttggttcag cttttatgaa ctcaacacta tttgcaaaat tgaaattagg ttctaaaaaa
    12841 gatattatat ctgttgctat tgaaccatta acccaatcag acataatttc agctaatcca
    12901 atacctatat attctactaa tttctttgga ggagcctttt ctggtatcat tgtagccttg
    12961 atgggtcttg taaacatgac accaggtaca gctacacctg tggctggttt tgctataatg
    13021 tttgcttata atccagttca acaagttctt ataacagctg gtttatgtgc tttattcaat
    13081 gtaattgcag gatttttagg ttcaaaaata ttcagtacct ttaaagttct tactgctggt
    13141 gaaatacgtg gtactgatgt agaagaagaa attgcttaaa aaacaataag atttataaat
    13201 ttatatctca agtagtagag ttttatattg taggcaattt tattaattgc aaacgtttta
    13261 ttttttattt atgtttatga ttattatgtg tgttgaatat ttagataaat ttttatttat
    13321 tatttttatt tagatttttg tttagttttt tatttggttg atatattagg aataaaaatt
    13381 agtatagttt attactag
//
LOCUS       DBGJFF010000104        13369 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.105, whole genome shotgun sequence.
ACCESSION   DBGJFF010000104 DBGJFF010000000
VERSION     DBGJFF010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13369
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.105"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(2..538)
                     /locus_tag="ACOIAF_002878"
     CDS             complement(2..538)
                     /locus_tag="ACOIAF_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HGZ2669880.1"
                     /translation="MIKETHERGGYIFALLILPFINNLYLVKYDIAYKIVLIIIYIYF
                     AYLGSLFPDIDMRGSYISKKFMLIYKLFGSRFRHRGFTHSLIALLLIYSFFKSLTIFT
                     NNNIVFSCLSSGFIIGYLSHLCLDLITKEGIELFYPITINISLLPIKTNSKTEKFISK
                     LLNFIVIFLIGYQFYILL"
     gene            complement(651..1355)
                     /locus_tag="ACOIAF_002879"
     CDS             complement(651..1355)
                     /locus_tag="ACOIAF_002879"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HGZ2669881.1"
                     /translation="MKKKILIPVIMSLFIISQCITSFAFTPENNKFKVKPLPYAYDAL
                     EPYIDKETMKLHHDKHYQAYVDKLNAALEKYPELYNYSLCELLQNLDSLPKDIATTVR
                     NNAGGAYNHKFFFDIMTPEKTIPSESLKEAIDRDFGSFEKFKQEFQKSALDVFGSGWA
                     WLVATKDGKLSIMTTPNQDSPVSKNLTPIIGLDVWEHAYYLKYQNRRNEYIDNWFNVV
                     NWNGALENYKNLKSQD"
     gene            1528..3402
                     /gene="asnB"
                     /locus_tag="ACOIAF_002880"
     CDS             1528..3402
                     /gene="asnB"
                     /locus_tag="ACOIAF_002880"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HGZ2669882.1"
                     /translation="MDGNISIYYKGDISKKEDIKFKIKESVNNTRLSSRIEYKNEEKT
                     FDFERFNVIDAIYGNKPFEKDGKVIVFNGNIYNYHEIKEELIKKGHNFTTNDDIEVLL
                     ASYIEFGKNCVHKIKGMFNFIIYDRENESIFGARDLFGIKPLYYINKENAIIFSSEYK
                     FLLEYMKNLNINERSLQSYFSFQYVLPEDTMIQGIRLIPAGHYFRVENGILSLKRYNK
                     LEFRSSTKFFYTKNHLGNRDVSEDDIRNIVVDSIVTHMEEEKEIGTFLSGGIDSSIIT
                     TVASQINPNIKSFSAGFSVKGYSELEVAKKTADKLGIENIQINITQDEYIKSLPNVIY
                     SLDDPIADPSEVGLYFLIKEAGKHVKVALSGEGADELFGGYNIYKEYSTMKSVVNSPT
                     YIKSILGRVSELMPNIKGRNYLYRATTPLEKRYIGNAKVFENSEVKRFFFKYKEKNIY
                     EYLLSNLYRDAQKNNYDYISKMQHIDVNTWLQGDILQKISKLSTAEQVELRVPFLYKD
                     VFDVAKNLRMDQKINKNNTKVLLREAFREIVPEHVVQRKKLGFPTPIRVWLKDSLGDI
                     VKETIYNSNVDEFIDKKYAIKLLDTHLKGHRDNSRKIWTIFTFCLWHQLFIEHKNVEY
                     "
     gene            complement(3494..4930)
                     /locus_tag="ACOIAF_002881"
     CDS             complement(3494..4930)
                     /locus_tag="ACOIAF_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HGZ2669883.1"
                     /translation="MICTIKPLKISIKKSTEPLIEFISDTTYKNKSSSYIKEVYFTRY
                     IGVDDSIDKYIEKIKNIDTYFFNNPTIPYIRLNNLKINFDKEQTDKMLKIFNIYDSSK
                     FNSWDLYNIDFPCKIENDTLNWTKKIAFKNTLDLFSTSMPNCTSSIIKNFAVKLLCWI
                     DYFLPKLFYNKVKTTISPKIVYFGNIKRQELFFLYFLSQLGCDILYINPNKDILDLYP
                     ECSKFSTLFELSRKTPNILEIPFERHITNINYSENTNKTSPTTNKPNSKNTLNNTNLT
                     SKKIVQDEEVELSYEDLAKLSASVVMIVVCNNENKPFKSGSGVVINNEGYILTNLHVV
                     NDGYSFLVRFENDDKVYTSYQIIKYHSDYDLAVIKVDRKCKPIPVKVSKKPVRGQKIV
                     AIGSPLGLFNTVSDGIISAFRDFETVQMIQFTAPISSGSSGGALLDMFGNLLGLISAG
                     YDDGQNLNLAVESNLVKIFANNFIEIVN"
     gene            5256..5837
                     /locus_tag="ACOIAF_002882"
     CDS             5256..5837
                     /locus_tag="ACOIAF_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HGZ2669884.1"
                     /translation="MMGITLSKGQKVDLTKGNPGLKNILVGLGWDTNKYDGGFDFDLD
                     TAAFLTGASGSVTNDGDFVFYNNLKHTSGAVEHLGDNRTGEGDGDDEQIVVDLSKIPG
                     EISKISFTVTIHDATERRQNFGQVSNSYIRIVDKDTNEELIKYELGEDFSIETAIVVA
                     EIYKHNGEWKFNALGSGFEGGLAALCGNFGINL"
     gene            5876..6454
                     /locus_tag="ACOIAF_002883"
     CDS             5876..6454
                     /locus_tag="ACOIAF_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HGZ2669885.1"
                     /translation="MAIVLKKGQKIDLTKGNPGLKNIKLGLGWDTNSFDSGYDYDLDV
                     SVFMVGESQRVEKDEDFIFYNNLKHPSGAVEHLGDNRTGEGDGDDEEILVNLETMPKH
                     IQKIAVTVTIYEAAERRQNFGQVSNSYIRVLNSENDEEILRYDLGEEFSIETAITVCE
                     IYKYNGEWKFSAVGAGFEGGLEALCRNYGLNV"
     gene            6540..7169
                     /locus_tag="ACOIAF_002884"
     CDS             6540..7169
                     /locus_tag="ACOIAF_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HGZ2669886.1"
                     /translation="MSINLSKGDKIDLKKSNPGLSNILVGLGWDPVQQSGGGFFKSLF
                     GGGQADIDCDASVFMLNQEGKLSGIKDLIYFGNLKSVCKSVLHTGDNLTGEGTGDDEQ
                     ILVNLDKVPSNIHKLLFVVNIYNCVDRKQHFGMIENAYIRVEDQGNKKEIAKYNLSDN
                     YSEKTTLIVGAIYRKDGSWQFKAIGEGTKDAGLKEVMQNLDRIECAYGI"
     gene            7159..9918
                     /locus_tag="ACOIAF_002885"
     CDS             7159..9918
                     /locus_tag="ACOIAF_002885"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005388 - P-type calcium transporter
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HGZ2669887.1"
                     /translation="MEFNNDIKYYNVDKEEVTKDLSVNPEKGLSESEVKTRREKYGLN
                     EFTPKEEGSFWDDLKESLSEPMIVILIIAAVVSAVIGETHDAIGIVGAIAIGIAIGMI
                     TEGRSKKAAEALSKLTENIEVKVLRDGEVHQISKSELVPGDIVYIETGDMVPADGRLI
                     ESINLKIREDMLTGESDDVSKKCDVVVSMEEIESKGAVVVQEPIPAKQINMVFGGTLV
                     AYGRGALVVTSIGDNSEMGKIAQNLSETDEQTPLQIKLGNLGGLIAKVSSAVAGLLFI
                     FMVFQMISKNVLNVDMSGILPFLESIDPVKTAFTVCVALIVAAVPEGLPTMINMTLAI
                     TMQKMAKINALVTKKEACETIGSVSVICSDKTGTLTENRMTVEVAYVDGRYIESSEEE
                     INSYFEENCMINSTADVEHNDGDIKYLGSATECALLLYYKNVDYRQVRKNSNIVAQNP
                     FTSDSKRMTSVIGQDNHHVLLSKGAPEVLLELCSHIQRGKDIVPLTENIKHEILEEIK
                     KLQIKSMRTLGFAYKEISQAEEEAAVTAESDAMNVSAMENNLVFSGFVGIRDPLRKDV
                     IESVNTANKAGVSVKMLTGDNINTARAIGEELGLLKNNMRVVEASYIDTLDDEELKQE
                     IQSISIVARSKPDSKMRIVSALQKSGDVVAVTGDGINDAPALSKADVGIAMGISGTEV
                     SKNAADIILTDDSFSTIVKGIKWGRGIYDNFQRFVQFQLTVNVIAFLVAIISQVMGQE
                     MPFTTIQLLWVNIIMDGPPALALGLEPVRDHVLNRKPVDRKANIISKSMVYTIVLNAF
                     YITAILMLQSTFNFLGVDEAHKGTVMFALFAFSALFNAFNCREFNADSIFPNFTHNKL
                     ALQIIVLTGIAQVIFTQLFQDFFNSVAMDFMTWVKVLCVAASVIVVNEVVKCIIRLVK
                     PKKTA"
     gene            10005..11567
                     /locus_tag="ACOIAF_002886"
     CDS             10005..11567
                     /locus_tag="ACOIAF_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceG family protein"
                     /protein_id="HGZ2669888.1"
                     /translation="MGDSKVFEKIFSSQSDRGNYTPSKGYLSYFISYIGLEDEVLYNL
                     EIFKTKQNIDSKKDIALFTDVIANPSDFDIINYFKSGLQKYRTSMEDVDINILGFEEI
                     DYKIKQAMDRVLKEEEKEFTNDRVKQNFIVKIMAWIKIYIGALDINKNEAPKVIFYGD
                     IKKHEVYLLLILYLAGFDVLYLNPNSKSNINILKSERYNIEFEEANIIEEKISFEERV
                     ILGEKIDKSSVKKAFTVGAEASKRISEELLNDAGFIKPWQLQDRKIKNLLLSSTVDEI
                     SIYWNQPLKLRPGFKFNDAIVEAPNFLSKINGIYNDKNEYIKFLDLLRDSESSTFIEF
                     NGDVDRFSKAFTREAFSLSFLLDSKGAIDKNSVLNNKDYSISTLALNQQIMILEKVEE
                     LLEGSMFLNGLSGEDKIKGLFTVLHMDKKFVHMMNNFDYSLINPKLIIYMYKSIVFDK
                     EIVFLMLLLSKIGFDIIILCPGGENNIENVINNQLIDVHRLDKMVYDLKLNSLENDIP
                     LLKKIFGKRRRF"
     gene            11568..12704
                     /locus_tag="ACOIAF_002887"
     CDS             11568..12704
                     /locus_tag="ACOIAF_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HGZ2669889.1"
                     /translation="MGININSFSKDKNSVEVTENLPTAVQEENFDIMEYTNNKKNELR
                     KSKEVEALTSLIEVENPDTILQFGRKASEGVARVSDSLLNTIKLNRNEENSKMLVHLT
                     KIMDKFDLDDFQETKEPNFVQKLFKKANNAIEMMFQKYETLGGEVEKIQIELEQYERD
                     IALSNKQIGAMLNENFEFYNELQKYIVAGEMAIEEMDNEILPFFKQKSETSGDQMDVV
                     NYQELLKVYDMLNQRVYDLRIAENIAIQTIPMLRGMQHNNYGLIRKINSAFVVTLPVF
                     KQCLSQAILLKKQELQAKSLKALDDKTNELLLRNAQNVSTQSAQIARMAGTSSVQIET
                     LEKMYNTIKSGIDETMRIEENNRALIKDNTKRLEELNTTIIYNK"
     gene            12768..>13369
                     /locus_tag="ACOIAF_002888"
     CDS             12768..>13369
                     /locus_tag="ACOIAF_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HGZ2669890.1"
                     /translation="MKYFDYICKDDLERIFLKEPEDFSAKTEKDVLKYALGAFLYVPA
                     TQYNMIYKSIIGDVKGVRPLAICLEDAVGVNGELEAIENLRLILKNISNESITNKDGI
                     PLIFVRIKDVEQLLRIKEIIIKNSHFITGILIPKANSELIENCIEALDSMNLQDMYVI
                     PIVETKEFIYNEKKELSFTNLYNAILRHKSRILSIRVGIT"
BASE COUNT         5269 a         1457 c         2314 g         4329 t
ORIGIN      
        1 actataataa aatataaaat tgatatccaa tcaaaaaaat aactatgaaa tttaatagct
       61 tagatatgaa tttttctgtt tttgagttag tttttatagg tagtaaagaa atgtttatag
      121 taatgggata aaacaattca ataccttctt tagtaataag gtcaagacac aagtgagata
      181 gatatcctat tataaaccca ctggacaaac atgagaaaac tatattattg ttagtgaaga
      241 ttgtcagaga tttaaaaaaa ctataaataa gtaataaggc aattagactg tgagtaaacc
      301 ctctatgtct aaacctactt ccaaataatt tgtatattaa cataaatttt ttacttatgt
      361 aagaacctct catatctata tctggaaata aagaacctaa atatgcaaaa taaatataaa
      421 ttataattaa tactatttta tatgcaatat cgtatttaac taggtacaga ttgtttataa
      481 aggggagtat caaaagtgcg aatatataac ctcccctttc atgtgtttct tttatcatat
      541 aaaaatcctc ctgaatcgtc actataaaca ttatattggt aaatttagac tatcttttat
      601 tatattatat agcaaacata agtttttttc aaaaggattt taatatttaa ttaatcttga
      661 gattttaaat ttttgtaatt ttctaaagca ccattccaat ttactacatt aaaccagttg
      721 tcaatgtatt catttcttct attttgatat tttaaatagt aagcatgttc ccaaacatca
      781 agtcctatta taggagttag gtttttactt acagggctat cctgatttgg agtagtcata
      841 atagataatt tgccatcttt agtagctaca agccaagccc aaccagaacc aaagacatct
      901 aaagcagatt tttggaactc ttgcttaaat ttttcaaaag aaccaaagtc tctatcaata
      961 gcttctttta aagattcaga aggtatggtt ttttctggcg tcattatatc aaaaaagaat
     1021 ttatgattat aagctccacc tgcattattt cttacagttg tagcaatatc tttaggtaaa
     1081 gaatctaaat tttgcaataa ttcacataaa gaataattat aaagctcagg atatttttca
     1141 agagcagcat ttaatttatc aacataagct tgataatgct tatcatgatg cagtttcatt
     1201 gtttctttat ctatataagg ttcaagtgca tcatatgcat aaggtaatgg tttaacctta
     1261 aatttgttat tttcaggtgt aaaagcaaat gaagttatgc actgtgagat tataaataaa
     1321 gacataataa ctggtattaa tatttttttc ttcataaaaa tcacctccta atatagtatt
     1381 tctagaaata aaaaaaatat gaaatattaa cagataattt ataagaattt aatagcaatt
     1441 tcattttaaa ttgaataagt tttaaagcca aagacaaaac tatgaaatat agaataagac
     1501 taaaggacag aaaggaattt tgattatatg gacggcaata tttcaattta ttataaaggt
     1561 gatatatcta aaaaagaaga tattaaattt aaaataaaag aatctgtaaa taatacaaga
     1621 ctttctagta gaatagagta taagaatgag gaaaaaactt ttgattttga aagatttaat
     1681 gtaattgatg ctatttatgg aaataaacca tttgaaaaag atggaaaagt tattgttttt
     1741 aatggaaata tatataacta tcatgaaata aaagaagaat taattaaaaa aggacacaat
     1801 tttactacaa atgatgatat tgaagtctta ttggcatctt atatagaatt tggtaagaat
     1861 tgtgtacata agattaaagg aatgttcaac tttataatct atgatagaga aaatgaaagc
     1921 atatttggag caagagattt atttgggata aaaccacttt attatataaa taaggaaaat
     1981 gcaataattt tttcctcaga gtataagttt ttacttgaat atatgaaaaa tttaaatata
     2041 aatgaaagaa gcttacaaag ttatttttca tttcaatatg tacttcctga agatactatg
     2101 atacaaggaa taaggctgat accagcaggt cactatttta gggtagaaaa tggtatacta
     2161 tctttaaaga gatataataa acttgagttt agaagtagta caaaattttt ttatactaaa
     2221 aatcacttag gcaatagaga tgtgagtgag gatgatataa gaaatatagt agtagattca
     2281 atagtaactc atatggaaga ggagaaagaa attggaacat ttttgtctgg tggaatagat
     2341 tcatctatta taactactgt ggcttcacaa attaatccaa atataaaatc tttttctgca
     2401 ggctttagtg tcaaaggata tagtgaatta gaagtagcaa agaaaacagc tgataagtta
     2461 ggtatagaaa atatccagat taatataaca caagatgaat atataaaatc attaccgaat
     2521 gtaatttatt ctttggatga ccctatagca gacccttcag aagtaggtct ttatttctta
     2581 attaaagagg caggtaaaca tgttaaagta gcattgtctg gagaaggagc agatgaatta
     2641 tttggaggat ataatatata taaagagtat agtactatga aatcagttgt caattcacct
     2701 acttatatta aaagtatatt aggaagagtt tcagaactta tgccaaacat aaaaggaaga
     2761 aattatcttt atagagctac aacaccatta gaaaaaagat atataggaaa tgcaaaagtt
     2821 tttgaaaaca gcgaagtaaa aagattcttt tttaagtata aggaaaagaa tatatatgaa
     2881 tacctgttgt ctaatttgta tagagatgca caaaaaaata attacgatta tataagtaaa
     2941 atgcaacata tagatgtgaa tacatggctt caaggcgata tacttcaaaa aattagcaaa
     3001 ctttctacag ctgagcaggt tgaacttaga gtaccatttt tatataaaga tgtatttgat
     3061 gtagcaaaaa atttaagaat ggatcaaaaa ataaataaga ataatacaaa agttttactt
     3121 agagaggcgt ttagagaaat agtcccagaa catgttgttc aaaggaaaaa attaggattt
     3181 ccaactccaa taagagtttg gttaaaagat tctctagggg atattgtaaa ggaaactatt
     3241 tataattcaa atgtagatga atttatagat aaaaaatatg caataaaact tttagatacg
     3301 catcttaaag gtcatagaga taattcgaga aaaatatgga ctatatttac attttgtcta
     3361 tggcatcaat tatttattga gcataaaaat gtcgagtatt aatactgttt tcaatattaa
     3421 tcagagggca aaagcataca atgttttcac cctcttgatt attatggaat aggataagta
     3481 gaattttgat tgattagttt actatctcaa taaaattatt ggcaaatatt ttgacaagat
     3541 tactttcaac agctaaattt aagttttgtc catcatcata tccagcggaa atcaatccta
     3601 gtaagtttcc aaacatatct aaaagagctc ctcctgaact accactagag attggagctg
     3661 taaattgtat catttgaacc gtttcaaaat cacgaaaagc tgaaataatt ccatctgaaa
     3721 ctgtattaaa aagacctaga gggcttccaa ttgcaactat tttttgacct ctcacaggtt
     3781 ttttactgac tttaacaggt attggcttgc attttctatc aacctttatc acagctaaat
     3841 cataatctga atggtatttt ataatttggt aagatgtata aactttatca tcattttcaa
     3901 atctaactaa aaatgaatat ccatcattga ctacatgaag atttgtaagt atataacctt
     3961 cattatttat tactactcca gaaccgctct taaacggttt attttcgtta ttgcacacaa
     4021 caatcataac aacagaagct gataactttg caaggtcttc ataagaaagt tctacttcct
     4081 catcttgtac aattttttta gatgttagat ttgtgttatt aagtgtattt ttagagttag
     4141 gtttattcgt agtaggagaa gtcttattgg tattttcact gtaatttata tttgtaatat
     4201 gcctttcaaa aggtatttct aatatatttg gagtcttcct agaaagttca aatagagtag
     4261 aaaattttga acattcagga tataaatcaa gaatatcttt atttggattt atatataaaa
     4321 tatcacaccc aagttgtgat aaaaaatata agaaaaaaag ttcttgcctt ttaatatttc
     4381 caaagtatac tatctttggt gaaatagtag tcttcacctt gttataaaat aatttaggta
     4441 gaaaatagtc tatccaacac aaaagtttaa ctgcaaagtt tttgatgata gaggatgtac
     4501 aatttggcat acttgtggaa aataaatcta aagtattttt aaatgcaatc tttttagtcc
     4561 aatttaaagt gtcattttct attttacatg gaaagtcgat attatataaa tcccaactgt
     4621 taaacttaga actatcataa atattaaaaa ttttaagcat cttatctgtt tgttctttgt
     4681 caaaatttat ttttagattg tttaatctaa tatagggaat tgtaggatta ttaaaaaaat
     4741 aagtatctat atttttaatt ttttctatgt atttatcaat tgaatcatca acacctatat
     4801 atcttgtaaa gtagacttct ttaatatatg aagaactctt atttttatag gtagtatcag
     4861 atataaattc aattagaggt tcagtagact tttttattga aatctttaat ggtttaatag
     4921 tacaaatcat attttttctc ctataataag atgatataat ctattataaa acaacttaaa
     4981 acaatatagt acaataattt tgtagtttca aaataaaata tgtacttgtt tatataaatg
     5041 atatataatt acatattaaa catagctttt atgcatttaa taaagtatgt aaaatctatg
     5101 taaaaagtaa gaaatataaa cattttttta attatatttt aaaaatatca agcaagtttt
     5161 cttcacaaat gaataaaaaa tgttataata tgattaaaga aaaagataaa taattagcaa
     5221 gatttgttta gctaaaatta acaagggagc gataaattat gggtataaca ttatcaaaag
     5281 gccaaaaggt tgatttaact aaaggaaatc caggtcttaa aaatatttta gtaggtttag
     5341 gatgggatac taacaagtat gatggtggat ttgactttga tttagataca gcagcatttt
     5401 taacaggagc aagtggaagt gttactaatg atggagattt tgtattttac aacaatttaa
     5461 aacatacatc tggagcagtt gagcatctag gagataacag aactggcgaa ggtgatggag
     5521 atgatgaaca aatagttgtt gatttatcta agattcctgg agaaataagc aagatttctt
     5581 ttactgttac aattcatgat gcaacagaaa gaagacaaaa ttttggacaa gtaagtaatt
     5641 cttatataag aatagtagat aaagacacaa atgaagagtt aataaagtat gaattaggag
     5701 aagattttag tatagaaaca gctatagtag tagctgaaat atataagcac aatggagaat
     5761 ggaagtttaa tgcactaggt tctggctttg aaggtggtct tgctgcatta tgtggaaact
     5821 ttggaataaa cttataattt caactataac aataaactaa agggggatat tttccatggc
     5881 tatagtatta aaaaaaggac aaaaaatcga tttaactaag ggaaatccag gtcttaaaaa
     5941 tataaaactt ggactaggat gggatacaaa ttcatttgat agtggctatg attatgattt
     6001 agatgtaagt gttttcatgg ttggagaatc tcaaagagtt gaaaaagatg aagattttat
     6061 attctacaat aacttaaaac atccatctgg agcagttgaa cacttaggtg ataatagaac
     6121 tggtgaaggt gatggagatg atgaggaaat attagtgaat ttagagacaa tgccaaagca
     6181 tatacaaaag atagctgtaa cagttacaat atatgaggct gctgaaagaa gacaaaattt
     6241 tggacaagta agtaactcat atataagagt attaaattct gaaaatgatg aagaaatatt
     6301 aagatatgat ttaggtgaag aatttagtat agaaactgca attacagtat gtgagatata
     6361 taagtataat ggagaatgga agtttagtgc agttggagca ggatttgaag gtggcttaga
     6421 agcactttgt agaaactatg gcttaaatgt ttaatttaag caatataata tattaaatat
     6481 aaataaccta tacaaataat gtataggtta tattatataa aattttgtgg aggtttagta
     6541 tgtcgattaa tttatcaaaa ggtgataaaa tagatttaaa aaaatctaac ccaggactga
     6601 gtaacatact tgtaggtcta ggatgggacc ctgtgcaaca atctggcgga ggttttttta
     6661 aatcgttatt tggtggagga caagctgata tagattgtga tgcatctgta tttatgctta
     6721 atcaagaagg taaattaagt ggaataaaag atttaattta ttttggaaat ctaaaaagtg
     6781 tctgtaaatc agttttgcat acaggagaca atctaactgg tgagggtaca ggtgatgatg
     6841 aacaaatcct agttaatttg gataaagttc catcaaatat tcataaatta ttgtttgtag
     6901 taaatatata caattgtgta gatagaaaac aacactttgg aatgatagaa aatgcctata
     6961 taagagttga agaccaaggt aataagaaag agatagcaaa atacaatcta tctgataatt
     7021 attctgaaaa aactactctt atagtaggag ctatatatag aaaagatgga agttggcaat
     7081 ttaaagcaat tggtgaagga acaaaagatg cgggtcttaa agaagtaatg caaaatttag
     7141 acaggattga gtgtgcatat ggaatttaat aatgatatca aatattataa tgtagataaa
     7201 gaagaagtta caaaagactt atctgttaat cctgaaaaag gtttatcaga atcagaagtc
     7261 aagactagaa gagaaaaata tggtttgaat gaatttactc caaaagaaga gggtagtttt
     7321 tgggatgatt taaaggaaag tttaagtgaa ccaatgatag ttatcttgat tattgcagca
     7381 gtagtaagtg cagtaattgg ggaaactcat gatgcaatag ggattgtagg tgctatagca
     7441 ataggtatag caataggtat gattacagaa ggtcgttcta agaaagcagc agaggctctt
     7501 tctaagttga ctgaaaacat agaagtaaaa gtattgagag atggggaagt acatcaaatt
     7561 tctaagagtg aattggtacc aggtgatata gtatatattg aaactggtga catggtacct
     7621 gctgatggta gacttataga aagtataaac cttaaaatta gagaagatat gttaacagga
     7681 gaatctgatg acgtatctaa aaagtgtgat gtagtagttt ctatggaaga gatagaatct
     7741 aaaggagcag tagtagtaca ggagccaata cctgcaaaac agataaatat ggtgtttggt
     7801 ggtacacttg tagcttatgg tagaggagct ctagttgtaa cttcaattgg agataatagt
     7861 gaaatgggta aaattgctca aaacttatca gaaacagatg agcaaactcc acttcagata
     7921 aaacttggaa atttaggtgg tttgatagcc aaagtttcta gtgcagtagc aggattacta
     7981 tttatattta tggttttcca aatgataagt aagaatgtat taaatgtaga tatgtctgga
     8041 atattaccgt ttttagaatc aatagaccca gttaaaacag catttacagt atgtgttgcg
     8101 ttgatagttg cagcagtgcc agaaggtctt ccaactatga taaatatgac acttgcaata
     8161 acaatgcaaa aaatggctaa aataaatgca ttggttacca aaaaagaagc ttgtgaaacg
     8221 ataggttctg tttctgtaat atgttcagat aaaactggta cattaactga aaatagaatg
     8281 actgtagaag ttgcatatgt tgatggcaga tatattgaaa gttcagaaga ggaaataaat
     8341 agttattttg aagaaaactg tatgataaac tctacagctg atgtagagca caatgatggt
     8401 gatattaagt atttaggaag tgctactgaa tgtgcattgc ttttatatta caaaaacgta
     8461 gattatagac aagttagaaa aaattctaat atagtagctc aaaatccttt tacttcagat
     8521 tcaaagagaa tgacttctgt tataggtcaa gataatcatc atgtactatt atcaaaagga
     8581 gcaccagaag tattacttga attatgttca catattcaaa gaggaaaaga catagttcca
     8641 ttaactgaaa atataaaaca tgagatttta gaagaaataa agaaattgca aataaaatca
     8701 atgagaactt tagggtttgc atataaagaa atctcacaag ctgaagaaga agcagcagta
     8761 actgctgaaa gtgatgctat gaatgtaagt gcaatggaaa ataatttagt atttagtggt
     8821 tttgttggta taagagaccc acttagaaaa gatgttatag agtctgtaaa tacagcaaat
     8881 aaagctggtg tttctgtaaa aatgcttaca ggagataata taaatacagc aagagctata
     8941 ggtgaagagt taggcctatt aaaaaataat atgagagtgg ttgaagcttc atatatagat
     9001 actttagatg atgaagaatt aaaacaagag atacaaagta tttcaatcgt ggcaagaagt
     9061 aaaccagata gtaaaatgag aatagtttca gctctgcaaa agagcggaga tgttgttgca
     9121 gtaacaggtg atggtataaa tgatgctcca gcactttcaa aagcagatgt tggtatagca
     9181 atgggtatat ctggtacaga ggtatctaaa aacgcagcag acataatttt aactgatgat
     9241 agttttagta ccattgtaaa aggtattaaa tggggtagag gaatttatga taacttccaa
     9301 agattcgttc aattccaatt aactgtaaat gtaattgcat ttttagtggc tataatatct
     9361 caagttatgg gacaagagat gccatttact acaatacaat tattatgggt aaacatcatt
     9421 atggatggac caccagcact agcattaggt ttagagccag ttagggacca tgtgttaaat
     9481 agaaaacctg ttgatagaaa agccaatata atttctaagt caatggtgta tacaatagta
     9541 ttaaatgcat tttatataac agcaatacta atgttacaat ctacatttaa tttcttagga
     9601 gttgatgaag cgcataaggg aacagtaatg tttgcgttat ttgcttttag tgcactgttt
     9661 aatgcattta actgtagaga gtttaatgca gatagtatct tccctaattt tacacataac
     9721 aaattagcac tacaaataat agtgttgaca ggaattgcac aagtaatatt tactcagcta
     9781 ttccaagatt tctttaactc tgtagcaatg gattttatga catgggtaaa agttttatgt
     9841 gtagcagcaa gtgtaattgt ggttaatgaa gttgtaaaat gtataataag actggtaaaa
     9901 ccaaaaaaga ctgcttaaaa caaatttttt agaagaaaac agactgactc aaaatatgaa
     9961 ttttgggtca gttctgaata aacatttaaa ttgggaggat agttatgggt gactcaaagg
    10021 tatttgaaaa aatattctcc agccaatcag atagaggaaa ttacactcct tcaaagggat
    10081 atctaagtta ttttatttcg tatattggtt tagaagatga agttttgtac aatctcgaga
    10141 tatttaagac aaaacaaaat attgatagca aaaaagatat tgctttattt actgatgtta
    10201 tagcaaatcc tagtgatttt gatattataa attattttaa aagtggactt caaaaatata
    10261 gaactagcat ggaagatgta gatataaata ttttgggatt tgaagagatt gattataaaa
    10321 taaagcaagc aatggataga gtgttaaaag aagaggaaaa agaatttact aatgatagag
    10381 ttaaacaaaa ttttatagtg aaaataatgg catggataaa aatttatatc ggtgctttag
    10441 atataaataa aaatgaagct ccaaaagtaa tattttatgg agatataaaa aaacatgaag
    10501 tttatttact cttaatatta tacttggcag gttttgatgt tttatatctt aatccaaatt
    10561 caaagagtaa tataaatatt ttaaaatcag aaagatacaa tatagagttt gaagaagcca
    10621 acataataga agaaaaaatt tcttttgaag aaagggttat tttaggggaa aaaatagata
    10681 aaagttctgt aaaaaaagct tttactgttg gggcagaagc atcaaagcgt ataagtgagg
    10741 aattgttaaa tgatgcagga tttataaaac cttggcaact ccaagataga aaaataaaaa
    10801 atctacttct aagttcaact gttgatgaaa tatcaatata ctggaatcaa ccacttaaat
    10861 taagaccagg atttaagttt aatgatgcta ttgtagaagc tccaaatttt ctttctaaga
    10921 ttaatggaat atataacgat aagaatgaat atattaagtt tttagattta ttaagagatt
    10981 cggaaagttc aacatttatt gaatttaatg gagatgtaga tagattttca aaagctttta
    11041 caagagaagc ttttagttta tcctttttat tggattcaaa aggtgcaata gataaaaact
    11101 ctgttttaaa taataaagat tattccatat caacactagc attgaatcaa caaattatga
    11161 ttttagagaa agtggaagag ttgttagaag gaagtatgtt tttgaatggt ttaagtggag
    11221 aagacaagat aaaaggtcta tttacagtac ttcacatgga taagaaattt gttcatatga
    11281 tgaacaattt cgattactca cttataaacc caaaattgat tatatacatg tacaagagta
    11341 ttgtatttga taaagaaata gtttttctta tgcttttact tagtaagatt ggatttgata
    11401 ttatcatatt atgtccagga ggagaaaaca atatagagaa tgttataaat aaccaattga
    11461 ttgatgtaca cagattggat aaaatggtat atgaccttaa attaaattcg cttgaaaatg
    11521 atataccttt acttaaaaaa atttttggaa agaggagacg attttagatg gggataaata
    11581 taaatagttt ttcaaaagat aagaactctg tagaggttac tgagaatttg cctacagcag
    11641 ttcaagaaga aaacttcgat ataatggaat atacaaataa taaaaaaaat gaactgagaa
    11701 agtctaagga agttgaggca ttgacatctc ttatagaagt agaaaatcca gatacgattt
    11761 tacagtttgg taggaaagca tctgaaggag tagcaagagt ttctgatagc cttttaaata
    11821 caataaaatt aaatagaaat gaagaaaatt ctaaaatgct ggttcattta actaaaataa
    11881 tggacaagtt tgatttagac gattttcaag aaactaagga accaaatttc gttcaaaaac
    11941 ttttcaaaaa agctaataat gccattgaaa tgatgtttca aaagtatgaa acattgggag
    12001 gagaagttga gaaaattcaa attgagcttg aacaatacga aagagatata gcactttcta
    12061 ataagcaaat aggtgctatg ttaaatgaaa actttgagtt ttacaatgaa cttcaaaaat
    12121 atattgttgc tggagaaatg gcaatagaag aaatggataa tgaaatacta cctttcttta
    12181 aacaaaaatc agagactagt ggagaccaaa tggatgttgt caattatcaa gaactattaa
    12241 aagtttatga tatgttaaat caaagagtct atgatttaag gatagcagaa aatatagcta
    12301 tccaaacaat acctatgctt agaggtatgc aacataataa ttatggttta attagaaaaa
    12361 taaatagtgc atttgtcgta actttaccag tatttaaaca atgtttatct caagctatat
    12421 tacttaaaaa gcaagaatta caagcaaaat ctttaaaagc acttgatgac aaaactaatg
    12481 aattattgct tagaaatgca caaaatgtaa gtactcaaag tgcacaaata gctagaatgg
    12541 ctggaacaag ttctgttcaa atagaaacat tggaaaaaat gtacaatact atcaaatctg
    12601 gtatagatga aactatgaga atagaagaaa acaatagagc tttaattaag gataatacaa
    12661 agagattgga agaattaaat acgactataa tttataataa gtagtattat attaagggct
    12721 aatttgtata aagttggccc tttctatgta tatgaggagg ctagacattg aaatatttcg
    12781 attatatctg caaagatgat ttagagagaa tttttttgaa ggagccagaa gattttagtg
    12841 ctaagactga aaaagatgtg ttgaaatatg cactgggcgc ttttttatac gttccagcta
    12901 ctcaatataa tatgatttat aagagtatta taggggatgt taagggagtt agaccattag
    12961 ctatttgttt agaagatgct gttggagtaa atggagagtt agaggctata gagaatctta
    13021 gattaatttt aaaaaatatt agcaatgaaa gtataactaa taaagatgga ataccactaa
    13081 tttttgtaag aataaaagat gtggagcaac tgttgagaat taaagagatt ataattaaaa
    13141 atagtcattt tataacagga atattaattc ctaaagcaaa ttcggagtta attgaaaatt
    13201 gtatagaagc tttagattct atgaatcttc aagatatgta tgttattccg atagtagaga
    13261 caaaagaatt tatatataat gaaaaaaaag aacttagttt tactaatttg tacaatgcta
    13321 ttttaagaca taagtcaaga atattaagca ttagagtagg cataactga
//
LOCUS       DBGJFF010000105        13304 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.106, whole genome shotgun sequence.
ACCESSION   DBGJFF010000105 DBGJFF010000000
VERSION     DBGJFF010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13304
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.106"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            274..903
                     /locus_tag="ACOIAF_002889"
     CDS             274..903
                     /locus_tag="ACOIAF_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HGZ2669891.1"
                     /translation="MSIKERRKNEKEEMKKKIMDASIEIINQHGYENLSIRKIATKIE
                     YSPTTIYLYYKDKAEIISDMTNKLYKTVESNAIDIMNNCSSLPIDKQIIEIMTSFIKT
                     LSSEPEMAKSIMHSRMNIIFASENTNNTPSNNGIKMLDKFLSIGIEQGIFKKNIDKSS
                     WMLISALLGFVLCVVENKLYSLHNFSQLIENFLDILVGGLYNENSQQGY"
     gene            878..1282
                     /locus_tag="ACOIAF_002890"
     CDS             878..1282
                     /locus_tag="ACOIAF_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HGZ2669892.1"
                     /translation="MKIRNKATNKKKKYVKISIALFFVFICIIIVIVSNLKPENLVVK
                     DIDINNVKNGIYTGSADNNLVKATVSVEVNNGKIQNINILKHDHLLGKPAEKITTSII
                     KQQSLDVDAITSATYSSNTIRKAVENALRKGE"
     gene            1285..1758
                     /locus_tag="ACOIAF_002891"
     CDS             1285..1758
                     /locus_tag="ACOIAF_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HGZ2669893.1"
                     /translation="MNTIIIYSSKYGCTKDCANILKNKLSDNVTIVDINNNNKIELSK
                     FDKIIIGSSIYVGSVSKKIQVLCNDNVELLNKKQVGIFLCCGFSEQADKYLKSNFPSS
                     LLESANAIGIFGSEARLEKMKFLDKLIMKAVSKGNYDSFRISQDNIDNFLINLNS"
     gene            2328..2993
                     /locus_tag="ACOIAF_002892"
     CDS             2328..2993
                     /locus_tag="ACOIAF_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HGZ2669894.1"
                     /translation="MIKLIGILIVVIGFILKIDTLFTVLLAGVATGIVAGLDFNQIFT
                     ILGDSFVSNRGVSLFILTLPVIGVLERYGLKQRAVSLIEKLKRLTTGKVLTIYMIARQ
                     IAGALSIRMSGHPQFVRPLVNPMAQAAGLSNSNELKESDEEAIKALSAASENYGNFYG
                     QNLFAGSSGVLLIASTLTQFGYNVTGLNIVKASTIMAVIALVVATIQFTLYDKKLNKS
                     HKK"
     gene            3015..3947
                     /locus_tag="ACOIAF_002893"
     CDS             3015..3947
                     /locus_tag="ACOIAF_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HGZ2669895.1"
                     /translation="MQNFINISLEIFYALMGFLMIVIAYKSFTTINNNKKYGTSLFWI
                     LISLPFIFGRLIPANIIGIILILSSLLTLSKQVVFAKYEEPDENFGKEQADKLKNKIF
                     IPSLILAFAAVVVAMSLSNFDNSSQFAIGVGSIIALISALIITKAKPATSVQDGSRLL
                     QQMGPASMLPQLLVALGALFTQAGVGEVISTMISGVVPADSRLFGVIAYVLGMVIFTM
                     IMGNAFAAFSVITAGIGIPFVLSQGGNPAIIGALALTAGYCGTLLTPMAANFNIVPAA
                     LLECKNDYIVIKYQAPVALVLIIAHILVMYFLGF"
     gene            3968..4609
                     /gene="pcp"
                     /locus_tag="ACOIAF_002894"
     CDS             3968..4609
                     /gene="pcp"
                     /locus_tag="ACOIAF_002894"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HGZ2669896.1"
                     /translation="MKILLTGFDPFGGEPINPAQEAVERVNNNINGAEIIKITIPTVM
                     TKSVEVIDKAIQEHNPDIVISVGQAGGRFDITPERVAINIDDFRIKDNEGNQVIDTII
                     KEDGEPAYFSKLPVKAMVKHMNENKIPASVSNTAGTFVCNHVMYGILYMIDKKYPNIR
                     GGFIHIPYTTSQVIDKKNTPFMSLEEIVKGLELAIEACIIYKEDVKEIGGEIS"
     gene            complement(4729..5061)
                     /locus_tag="ACOIAF_002895"
     CDS             complement(4729..5061)
                     /locus_tag="ACOIAF_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HGZ2669897.1"
                     /translation="MSIDCISNTDLSETGFSYTLSLISGKYKMIILYCLVEFEVVRYN
                     ALKRYINTISYKTLSLSLKELEADNLIIRTEYPQIPPKVEYSLSERGKSLIPILDAMC
                     EWGERNRP"
     gene            5327..5947
                     /locus_tag="ACOIAF_002896"
     CDS             5327..5947
                     /locus_tag="ACOIAF_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HGZ2669898.1"
                     /translation="MKVLLINGSPNQYGCTYTALNEITKVLSKHDIKTEILYLGKETI
                     PGCISCASCFETGKCIRNDKVNELIEDLDNIDGIIIGSPVYFSSATGQLTSFLDRLFF
                     IAGSRMATKLGASVVSCRRGGASATFDQLNKYFSISNMPIVSSQYWNQVHGFTPEDVM
                     KDEEGLQTMRTLGENMAWLLKCISAGKKAGIKEPQYEETIMTNFIK"
     gene            complement(6272..7096)
                     /locus_tag="ACOIAF_002897"
     CDS             complement(6272..7096)
                     /locus_tag="ACOIAF_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraX family protein"
                     /protein_id="HGZ2669899.1"
                     /translation="MDFFKKGISGFTIKILALIFMTFDHIAAFMPQTMQIPIWFHWVG
                     RISAPLFIFMAVEGFYHTSNRKKYISRLYIWSVIMAIGNQIINNVFSHPEGAIIINNI
                     FSTLFLIAIYLQAIEFIKKFRKEKEIKYFIIGLLMIIIPIILGIFTVALLFKVTNRVI
                     ALFMILVPVPFLVEGGPIWIILGIIFYLCRGKKFSLSICYALMCIFIFTTMSNGDYSL
                     KNSILQNYQWMMIASLPLMLLYNGEKGKSMKYLFYLYYPIHVYILYILGIYLINGF"
     gene            complement(7107..8510)
                     /locus_tag="ACOIAF_002898"
     CDS             complement(7107..8510)
                     /locus_tag="ACOIAF_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGZ2669900.1"
                     /translation="MKWKITRNFIFTIVFVAISVVIINIISILYVISTNSFFKVVDSG
                     NNPEEFARSFEKDLYEKDGEFKLSKIGAEKLEKSNSWIQVLNDLGEEVYGVNVPKDTP
                     KKYTPFQMVNNYKYIETKYVNFVLEKHLNKKHLNIIVGIPSRDISRIILTYSQNNIKK
                     TLNKVIIITLVIDSVVALGVGYLFSRKLTKPISSVLWSIETMANGNYSLYLKDRGIYE
                     EVFKNINMLADTLRVNEVERKENEELREEWLANITHDIKTPLASIQGYAEIINDKDYE
                     FEEDEIQEYTEIIYNKSKYIKDLVDDLNLSTRLKNNTIVLDKKKINLVSLVRNIIIDI
                     LNDNRYKNRNIEFESNEDLIEVYIDSILFRRAITNLIFNSIVHNSEGTLISVEIVKKD
                     NIEIIIKDNGIGISKSDLKHIFKKYYRGTNTGEMHKGSGLGMAISKEIIEIHKGKIYV
                     SSEIGIGTKIIIEIKQN"
     gene            complement(8514..9239)
                     /locus_tag="ACOIAF_002899"
     CDS             complement(8514..9239)
                     /locus_tag="ACOIAF_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGZ2669901.1"
                     /translation="MENRVLIIDDEVEILKLLETVLKKEGLNNIYTAKTKKEGLELFK
                     SINPDLIVLDIMLPDGEGYDICKEIRKTSNSPIIFLSAKTEELDKLLGLAIGGDDYVT
                     KPFSPKEVAFRVKAHLRRLSYFSDAQNESKNLNNNEEKIISFGPYILNESRAELIKDG
                     KSIGLSAKELKILSLFAHNQNQIISKEKLWDKVWGEDYVGFDNTIMVHIRKIREKLED
                     NPSKPEYILTIKGLGYKLAVKED"
     gene            complement(9351..10124)
                     /locus_tag="ACOIAF_002900"
     CDS             complement(9351..10124)
                     /locus_tag="ACOIAF_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2669902.1"
                     /translation="MMYLVKAEMIKILKSKSVWIIWLFLIFFGFFLIRKFDVLDTYAD
                     IFYKIEGSIPLIGLIMFIITSGNYIKEYDSNMTGLINTTKKGKKSVVLSKLVANGLIL
                     SIINISFILLVGIRGFSFFDFKNLNEPIHNLWYFGNSNLNLTVIQMYIIVILTTIFAS
                     FIFAQIGLTLSSIFKSAVIPFILGGLIMAIPYFSVGFIPDKAIKFMSVTPNWIMMSQQ
                     MVKYNVPSILIVFSIVISIILMIVLTKITYENFTSSKRF"
     gene            complement(10130..11017)
                     /locus_tag="ACOIAF_002901"
     CDS             complement(10130..11017)
                     /locus_tag="ACOIAF_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2669903.1"
                     /translation="MNKLEIENLSKIYGKKVANDKITVTLENGVYGLLGPNGAGKSTL
                     MKQITTLIKPDEGQILYNGEDIFKMDADYRNILGYLPQEFGVYKNFTAKEFLQYIGAL
                     KGLRGRYLNSKIGELLDLVGLYDVRNKSIGKFSGGMKRRVGIAQVLLNDPKIIVLDEP
                     TAGLDPQERARFRNLIAKMSRDKIIILSTHIISDIESVAKETIMIKDGKLLMKGTHKD
                     ILSHMENKVYNVQVKNECEVDDIQSKYKVVSIQSDINSIVLRIVSEDIPSEDNIQPVQ
                     AKFEDVYMFYFDLEDAREV"
     gene            complement(11031..12053)
                     /locus_tag="ACOIAF_002902"
     CDS             complement(11031..12053)
                     /locus_tag="ACOIAF_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669904.1"
                     /translation="MVNWEIKKLAKSKSMFISLGILVLILMISIFITPVLETESYYID
                     KEKGRIEDTRSGIDIGNEKFQNKINVLKQFSNQTDTGEFSKKIELMSKKKLDNLSVNE
                     YKDISFWKVFNYRVTNSLINVGMLIIISIIISNIYVDEVVTSVKDIILSSKEKKKALK
                     SKIFVALLAPLVIYSVYLMGIFIITCMQQGVPVNGDLESFRIVDNVVALKTNLSITKY
                     VILNIGIELLMFEGWAMIVIFGSFISKSSISSISFFIFIISITKIMSVIHILPKKLLS
                     VVSNVNYYDLIFGFNKIIGNYLGDIMIFKVNIDIVNIAIGLLMAILIGAMSLCFLVIK
                     SDYISR"
     gene            12628..12804
                     /locus_tag="ACOIAF_002903"
     CDS             12628..12804
                     /locus_tag="ACOIAF_002903"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669905.1"
                     /translation="MFIGEIDKCTHILTAYISSSYDYCNFLDTQLDDFISEYGETVVE
                     TCLHQVLLLVSRYN"
BASE COUNT         4904 a         1902 c         1537 g         4961 t
ORIGIN      
        1 aataaatttt atagcataaa agaatttatt atattttaat gtatcttatt aaagtttatt
       61 tagaattatg aaccaacttt aataagatgt ggcgcattta aaccgattat tattatacaa
      121 taatatctta aatattacaa ttaataacat taatttatta tccataaaaa tatattaact
      181 tatattattt aataatttat tgacagtatt aaaattaaat tatacaataa cagtgtaaat
      241 taacttaaca ccgttaagca aagaggtgat tatatgagta tcaaagaaag acgtaaaaat
      301 gaaaaagaag aaatgaagaa aaagattatg gatgctagca tcgaaattat aaaccaacat
      361 ggatatgaaa atttatcaat aagaaaaatt gctacaaaaa tagaatattc tccaactaca
      421 atttatcttt actacaaaga caaagcagag attatcagcg atatgaccaa taaactttat
      481 aaaacagtag aaagtaatgc tattgacatt atgaataatt gctcatcact accaatagat
      541 aaacaaatca tagaaattat gactagcttt ataaaaactc tttctagtga accagagatg
      601 gcaaaatcta tcatgcatag tagaatgaat attatatttg catctgaaaa tacaaacaat
      661 acgcctagca ataatggtat aaaaatgctt gataaattcc tttccattgg aattgaacaa
      721 ggaatattca aaaaaaatat agataaaagc tcatggatgc ttataagtgc attgttagga
      781 ttcgttttat gtgttgttga aaataaacta tattctttac ataatttttc tcaacttata
      841 gaaaatttct tagatatttt agtgggagga ctttacaatg aaaattcgca acaaggctac
      901 taataaaaag aaaaaatatg taaaaatatc tattgctcta ttctttgtat ttatatgcat
      961 tattattgta attgtaagca acttaaagcc agaaaacttg gtagtaaaag atatagatat
     1021 taataatgtt aaaaatggca tttatactgg aagtgctgat aataacttgg taaaagctac
     1081 tgtgtctgtt gaagttaata atggtaaaat ccaaaatatt aatatcttaa aacacgacca
     1141 tcttttaggt aaacctgctg aaaaaattac aacaagtatt attaaacaac agtctttaga
     1201 tgtggatgct ataacttctg caacttatag cagtaatact atacgaaaag ctgtagaaaa
     1261 tgctttaaga aagggagaat aattatgaat actattatta tatattcaag taaatatggt
     1321 tgtacaaaag actgtgcaaa tatactaaaa aataaactat cagataatgt cactattgtt
     1381 gatattaata ataataataa aattgagtta tcaaaatttg ataaaattat tattggaagt
     1441 tctatatatg ttggttcagt ttcaaaaaaa atacaagtgc tatgtaatga taatgtagag
     1501 ttattaaaca aaaaacaagt tggcattttc ctttgctgtg gcttttctga acaagcagat
     1561 aaatatttaa aatcaaattt tccatcatcc ttattagaaa gtgcaaatgc tattggaatt
     1621 tttggtagtg aagctcgatt agaaaaaatg aaatttttag ataaacttat tatgaaggct
     1681 gtttcaaaag gtaattatga cagttttaga atatcacagg ataatataga taatttttta
     1741 ataaatctta atagctaaaa taaaactaat tatttctatg ataaattctt ttatttataa
     1801 gtaatattgc tcaaaatagt ctttaaactt tatcaaaatt ttatttttct tagtgaaatc
     1861 taatgaaact agcacttaat atatgcttta ttaatcataa caaatatgtt ttattaatca
     1921 taacaaaaag agagtgtcta atgaactaaa aaattcatta gacactctct ttttgtatga
     1981 aatcaaatat atttttatca tttcaaaagt gaaaaataca cttgtctcta tgtttggcac
     2041 attctctttt tataatataa tatctaattt aaaacctatt tcaaatattg acatatactt
     2101 aaactataga cttacaaaat caattcatat tagcttaaaa tataatcttt gttattcaaa
     2161 ttcctataca caaccattat ttactttgat actttaaaac atcttcccta ttttttatta
     2221 aattttataa taatagtcct attaacgcaa atactataaa aagagtttaa ctttatccat
     2281 taaataaatt taaattaaaa catatagaaa tggaaggaga acaatttatg ataaaactaa
     2341 tcggtatatt gatagttgta attggattta tacttaaaat tgacacacta tttactgtat
     2401 tattagcagg agtggcaaca ggaattgttg caggattaga ttttaatcaa atttttacaa
     2461 ttttaggaga ttcttttgtc tcaaacagag gtgtatcatt attcatatta acattgccag
     2521 taatcggtgt attagaaaga tatggtttaa aacaaagagc tgtttcttta atagaaaaat
     2581 taaaaagact tacaacagga aaagtactta ctatatacat gatagctaga caaatagctg
     2641 gtgctctatc aataagaatg agtggacatc ctcaatttgt aagaccactt gttaatccta
     2701 tggcacaagc tgctggttta agtaatagta atgaactaaa agaatcggat gaagaagcca
     2761 taaaggcatt atctgctgca tcagaaaact atggaaattt ttatggtcaa aacttatttg
     2821 caggtagtag tggagtatta cttatagcat ctactctaac tcaatttgga tacaatgtaa
     2881 ctggacttaa tattgtaaag gctagtacta ttatggcagt aatagcactt gtagtggcta
     2941 caatccagtt taccttatat gataaaaaat taaacaaaag tcataaaaaa tagttttatt
     3001 taggaggaat aaaaatgcaa aattttataa acatatcatt ggaaatcttt tacgcactaa
     3061 tgggattttt aatgattgtt atagcatata agtcttttac taccataaat aacaataaga
     3121 aatatggtac atcattattt tggatactta tatcactgcc attcatattt ggtagattga
     3181 ttccagccaa tataatagga ataatattaa tattatctag tttattaaca ctttctaaac
     3241 aagttgtatt tgcaaagtat gaagaaccag atgaaaattt tggaaaagaa caagcagaca
     3301 aacttaaaaa taaaatattt ataccatcat taatattagc atttgctgca gtagtagtag
     3361 ctatgagcct ttctaatttt gataattctt ctcaatttgc aataggtgtg ggttcaataa
     3421 ttgcattaat atcagcgctt ataataacta aggcaaaacc tgctacatca gttcaagatg
     3481 gctcaagatt acttcaacaa atgggacctg ctagtatgtt accacaactt ttagttgcat
     3541 tgggagcttt atttactcaa gccggagtcg gtgaagttat ttcaaccatg atatctggtg
     3601 tagtacctgc tgatagcaga ttatttggtg ttatagctta tgtattaggt atggttatat
     3661 ttaccatgat aatgggaaat gcatttgccg cattttcagt cataacggca ggaattggta
     3721 ttccttttgt attgtctcaa ggtggaaatc ctgctataat tggagcatta gcattaacag
     3781 caggatattg tggaactcta cttactccaa tggctgctaa ttttaatata gttccagcag
     3841 ccttgctaga atgtaaaaat gattatattg ttataaaata tcaagctcca gttgctttgg
     3901 tattaattat agcccacata ttagtaatgt actttttagg attttaatat aattaggagg
     3961 taaaataatg aaaattttgt taacaggatt tgacccattt ggtggagaac caataaaccc
     4021 agctcaagaa gcagttgaaa gggtcaataa taatataaac ggtgcagaaa tcataaagat
     4081 aactatacca acagttatga caaaatctgt tgaagttata gataaagcta ttcaagagca
     4141 caatccagat atagtaatat cagttggaca agctggaggt agatttgaca ttacacctga
     4201 gagagttgct ataaatatag atgattttag aataaaagat aatgaaggta accaggttat
     4261 agatactatt ataaaagaag atggtgaacc tgcatatttt tcaaaattac ctgtaaaagc
     4321 tatggtaaaa catatgaatg aaaataaaat tccagctagt gtttcaaaca ctgcaggaac
     4381 attcgtttgc aatcatgtga tgtatggaat cctttatatg atagacaaga aatatcctaa
     4441 tataagaggt ggatttatac acatcccata tacaacttca caagtaatag ataaaaaaaa
     4501 tacaccattc atgtctttag aagaaatagt aaaaggctta gaattagcta tagaagcttg
     4561 tattatatat aaagaagatg taaaagaaat tggtggtgaa atttcataat aaatttttta
     4621 gtatttttat aaattaaaaa tactaactat taatttaaat atagactgtg agtagattat
     4681 tcaaacaatt ttactcacag tctttttatg gtatctacag atacaaaatt aaggtctatt
     4741 cctctctccc cactcacaca ttgcatctaa aattggaatc agagattttc ccctttctga
     4801 tagactatat tctaccttag gaggtatttg aggatattca gtacgaataa tcaagttatc
     4861 tgcttctaat tcttttaatg ataaactcaa agttttatag gagatagtat ttatataacg
     4921 ttttaatgca ttatatctta caacttcaaa ttctacaaga cagtaaagaa ttatcatctt
     4981 atatttacca ctaatcagtg aaagtgtata actaaatcct gtttcactta aatctgtatt
     5041 tgatatacag tctatactca tatattacac tctccttttg gttagtattg aacaaaaatg
     5101 tgcgtacttg tttttagaat aatttataat aaaattatag tgtaagaata attgaaagtc
     5161 aattttataa taggaggtgt atttactaaa aaaaaagtta ttttaaatag gattctaaga
     5221 ttagacattt ctatataaat tgataatatt catgaaaatc ctattttata aaaaataata
     5281 tctcttagaa aatctattaa ataaaattca tgggaggaaa tttataatga aagttttatt
     5341 gataaacggt agcccaaacc aatatggatg tacttataca gcactaaatg agattactaa
     5401 agtattgagt aaacacgata ttaaaacaga aatactgtat ttgggtaaag aaacaatccc
     5461 aggatgtatt agttgcgcat cctgttttga aactggaaaa tgtattcgaa atgataaagt
     5521 taatgaattg attgaagatc tggataatat tgatggtatt attattggtt ctcctgttta
     5581 cttttctagt gcaactggtc aattaacttc ttttcttgac cgtctatttt tcatagctgg
     5641 aagtcgcatg gctacaaaat taggtgcttc agttgtgtca tgtagacgtg gtggtgcaag
     5701 tgcaacattt gaccaactaa ataaatattt ttcaatcagc aatatgccta ttgtatcatc
     5761 acagtattgg aatcaggtac atggctttac tccagaggat gttatgaagg atgaagaagg
     5821 attacagact atgcgaactc taggagaaaa tatggcatgg cttcttaagt gtatatcagc
     5881 aggtaaaaaa gctggaatta aagaaccaca atatgaagaa acaattatga ctaattttat
     5941 aaaataaata aaaaatgcac taatattaat taaaacaaaa cactaattta taaataaaat
     6001 aagcataaat gtaggataaa aagcactaaa acagaggtat tgaattttat atcaaacctc
     6061 tgttttatta ttacattaat aaccaaacca tgtataacat catatatttc taaataaatt
     6121 ataatattat attctaatgt tattgaaatt acaataactg aatttctgct cataaattta
     6181 aatattctac aaatctcatc taaataacaa ttttaataga cctaaaacaa tcctcatatt
     6241 gttcattttg tattaaaaat atcacttaca atcaaaaacc atttattaag tatattccta
     6301 agatatacaa tatatataca tggattggat agtataaata aaataaatac ttcatactct
     6361 tccctttttc tccattgtaa agtaacatta aaggtagaga agcaatcatc atccattggt
     6421 aattttgtaa aatagaattt tttaaactat aatctccatt tgacattgtt gtaaatataa
     6481 atatacacat taaagcataa caaatactca aactgaactt tttccctcta cacaaataaa
     6541 aaataatccc aagtataatc caaattggtc caccttctac taaaaatggt actggtacca
     6601 aaatcataaa taaagcaata actctgttag tgactttaaa aagtaaagct actgtaaata
     6661 ttcctaaaat tataggaatg attatcatca ataatcctat aataaaatat ttaatttctt
     6721 tctcttttct aaatttcttt atgaactcta ttgcttgtaa ataaatcgct attaaaaata
     6781 atgttgaaaa aatgttattt ataattatag ctccttctgg atgtgaaaat acattattaa
     6841 ttatttgatt tcctatagcc ataataacag accaaatata caatctactt atatattttt
     6901 ttctgttact agtgtgataa aatccttcaa cagccataaa tataaacagt ggtgctgata
     6961 ttctgcctac ccaatgaaac caaattggta tttgcattgt ttgtggcata aatgctgcta
     7021 tatggtcaaa tgtcataaat attaatgcta aaatcttaat agtaaaccca cttatacctt
     7081 ttttaaaaaa atccatatca tatctcctaa ttttgtttta tctctataat aatttttgtt
     7141 cctattccaa tttcactact tacataaatt ttacctttat gaatctctat tatttcttta
     7201 gaaatagcca taccaagacc tgaaccttta tgcatctcac ctgtattagt acctctataa
     7261 tattttttaa aaatatgttt caaatcactt ttgcttattc caattccatt atcttttatt
     7321 ataatttcta tattatcttt ttttacaatt tctacactta tcaaagttcc ttcgctatta
     7381 tgaactattg aattaaatat aagattggtt atagcccttc tgaataagat tgaatctata
     7441 tatacttcaa ttaaatcttc attactttca aattcaatat tcctattttt atatctatta
     7501 tcatttaaaa tatcaattat tatatttctg actaaactga ctaaatttat ttttttcttg
     7561 tccagaacta tagtattatt ttttaatcga gtagataaat ttaaatcatc aactaaatct
     7621 tttatatact tagacttatt atatataatt tctgtatact cttgtatttc atcttcttca
     7681 aactcataat ctttgtcatt aattatctct gcataccctt gtatagatgc tagaggtgtc
     7741 tttatatcat gtgttatatt tgccagccat tcctctctca actcttcatt ttcttttctc
     7801 tcaacttcat ttactcttaa agtatcagct aacatattta tatttttaaa aacttcttca
     7861 tatatacctc tgtccttaag atataaacta taatttccat tagccatagt ctcaatactc
     7921 catagtacgc ttgaaattgg ttttgtaagt tttctactaa ataaatatcc tacacctaaa
     7981 gctaccacgc tatcaattac tagtgttata atgataactt tatttaaagt ttttttaatg
     8041 ttattttgag aataggttaa aattattctt gatatatctc tactaggtat acctactatt
     8101 atgtttagat gttttttgtt taaatgtttt tctagtacaa aattaacata ctttgtttct
     8161 atatacttgt aattattcac catttgaaag ggtgtatatt ttttgggagt atcctttggc
     8221 acatttactc cataaacttc ctctcctaaa tcatttaaaa cctgtatcca ggaatttgat
     8281 ttttctaatt tttcagctcc aatcttagat agtttaaatt ctccatcttt ttcatataaa
     8341 tctttttcaa aagaacgtgc aaattcttca ggattatttc cagaatctac aactttaaaa
     8401 aaactattag ttgaaatcac atacaatata gatatgatat ttataattac tactgatata
     8461 gctacaaaca caattgtaaa tataaaattt ctagtaatct tccacttcat attctaatct
     8521 tcctttactg ctaatttata tccaagacct tttattgtca gaatatattc aggttttgaa
     8581 ggattatcct ctaatttttc cctaattttt cttatatgaa ccattatagt attgtcaaat
     8641 ccaacataat cttctcccca cactttatcc catagttttt ctttacttat aatttgattt
     8701 tgattatgtg caaataaaga taggattttt agttcctttg cagacagtcc tatagatttt
     8761 ccatctttta taagttctgc cctactttca tttaatatat atgggccaaa tgatataatt
     8821 ttttcttcat tattatttag atttttactt tcgttttgag catcactaaa ataacttaat
     8881 cttctcaaat gcgctttaac tctaaaagcc acttccttag ggctaaaagg ttttgtaaca
     8941 taatcatctc ctccaatcgc tagtccaagt aacttatcta attcttcggt tttagctgat
     9001 aaaaatatta ttggagaatt ggaggttttt cttatttctt tacaaatatc ataaccttcg
     9061 ccatctggta acataatatc caatacaatc aaatcaggat taatactttt aaataattct
     9121 aatccttctt ttttagtttt tgcagtatat atattattaa gaccctcttt ttttaggaca
     9181 gtttctaaaa gcttcaatat ttctacttca tcatctatta ttaaaacacg attttccata
     9241 aacaacctcc tgtaataatt tacatatata ttagcatata ttttatctaa aattcattta
     9301 tcttattttt aattaaacta aaatataaat aaaattacta ttatttttag ttaaaacctt
     9361 ttgcttgatg taaaattttc atatgttatt ttagttaaca ctatcattaa tataattgag
     9421 attactatag aaaaaactat caaaatactt ggtacattat acttaaccat ttgttggctc
     9481 atcataatcc aatttggagt cactgacata aattttatag ctttatctgg tataaaacct
     9541 acactaaagt atggaattgc cattatcaat ccacctagta taaatggaat tacagccgat
     9601 ttaaatatag atgaaagtgt taagcctatt tgtgcaaata taaaagaagc aaaaatagta
     9661 gtcaatataa caattatata catttgtatt actgtaagat ttaaattact atttccaaag
     9721 taccataagt tatgaatcgg ttcatttaaa tttttaaaat caaaaaaact aaaacctcta
     9781 attcctacta acaatataaa agaaatatta ataatagata aaattaatcc attagctact
     9841 agttttgata atactactga tttttttccc tttttagttg tatttattaa acctgtcata
     9901 ttagaatcat attctttaat gtaatttcca gaagttatta taaacattat caaacctatt
     9961 agaggtattg agccttctat cttgtaaaat atatctgcat aagtatctaa aacatcaaac
    10021 tttctaatca gaaaaaatcc aaaaaatatt aaaaacagcc aaattatcca aacactcttt
    10081 gattttaata tttttatcat ttcagcttta actaaataca tcatttctac tacacctccc
    10141 ttgcatcttc taaatcaaaa taaaacatgt atacatcttc gaattttgct tgaactggtt
    10201 gtatattatc ctcgcttgga atatcctctg aaacaattct aagtactatt gaattaatat
    10261 cactttgaat acttacaacc ttatatttag attgtatatc atcaacttca cattcatttt
    10321 taacttgaac attatatact ttattttcca tgtgacttaa tatgtctttg tgtgttcctt
    10381 tcattaggag tttgccatct ttaatcatta ttgtttcctt tgcaactgat tctatatcgg
    10441 aaattatatg agtagaaagt attattatct tatctctaga catttttgct attaaattcc
    10501 taaatctagc tctttcttgt gggtcaagac ctgctgttgg ctcatcaagg actattatct
    10561 tagggtcgtt tagtaaaact tgtgctatcc caactctcct cttcatacct cctgaaaatt
    10621 ttcctataga cttatttctc acatcataaa gacctactaa atctaataat tctcctatct
    10681 tactatttaa atatcttccc cttaaacctt ttaaagctcc aatatactgt aaaaattctt
    10741 ttgctgtaaa atttttataa actccaaatt cttgtggcaa atatcctaaa atatttctat
    10801 aatcagcatc cattttaaat atatcctcac cattataaag tatctgacct tcatcaggtt
    10861 ttatcaaagt tgttatttgt ttcattaatg tagattttcc tgctccattt ggtcctagaa
    10921 gaccataaac tccattttcc agagttactg taatcttgtc gtttgctact tttttaccat
    10981 atatctttga aagattttct atttctaatt tattcatctt aactcccctt ttatctactt
    11041 atataatctg attttattac taaaaaacat aatgacatag cacctatcaa tattgccatc
    11101 aaaagtccta ttgctatatt gacaatgtct atattcactt taaatatcat aatatcacct
    11161 agataatttc ctattatttt attaaaacca aatatcaaat cataataatt tacatttgat
    11221 acaactgaaa gtaatttttt aggtaatata tggattaccg acattatctt agttatagat
    11281 ataataaata taaaaaaact tattgaagat attgaagatt tacttataaa agacccaaat
    11341 atgactatca tggcccatcc ttcaaacatc aaaagctcta tgcctatatt taatattaca
    11401 tactttgtta tacttaaatt agttttcaat gcaactacat tatctactat tctaaaagat
    11461 tctaagtctc cattaacagg tactccttgt tgcatacaag ttataataaa tattcccatt
    11521 aaataaacag aataaattac taatggagct aataaagcaa caaatatttt agactttaat
    11581 gcttttttct tctcttttga tgatagtatg atatctttta ccgaagtaac gacttcatct
    11641 acgtaaatat tggatataat tatagatata atgattaaca ttcctacatt tataagtgaa
    11701 tttgtaactc tatagttaaa tactttccaa aaactaatat ccttatattc attcacactt
    11761 agattatcaa gttttttctt tgacattaat tcaatttttt tagaaaattc acctgtatct
    11821 gtttgatttg aaaactgctt taaaacatta atcttatttt gaaatttctc attacctatg
    11881 tcgataccac ttctcgtatc ttctatacgt cctttttctt tatctatata ataactttct
    11941 gtttctaaaa caggcgtaat aaagatacta atcattagta ttaacactaa tatccctaga
    12001 gatataaaca tactcttaga tttagccaat ttttttattt cccaatttac caatatattt
    12061 ctcctcctaa atcattttaa ttacacatat atattaaaac ttaaatctta tataaatata
    12121 atactaacct taaataaagc ttaaattatt ttataattta taggtgtgtt tattattgga
    12181 gttacattta ttatttttct gggaaattat acattttcaa ttatttttat tatttttgat
    12241 atttttattg attggaaatt ttattgttgt tcaaattgtg gttattcttt aaatttaaag
    12301 atggagtttt ttaagaatag aacctactgt ccttattatg gaaagattat taaatagttg
    12361 catataactt tatttatctt gaatcttatt tttaaaaaca attataattt tagaatattt
    12421 tctattctca aacaagcatg ttgaataatg aagcatcttc tagtattata tacttgtaga
    12481 ataaaactaa attggtaaaa ctgggaatat ttaaccttaa ggagttaagt tatagaaact
    12541 agtactaaaa aattttataa attatgttaa ttgactaata gaatgcgact ttttattttt
    12601 actaaatttt ttagggggat ggataatatg tttattggag aaatagacaa atgtacacat
    12661 atactaactg cttacatcag tagttcatat gattattgta attttttaga tacacagtta
    12721 gatgatttta tatcagaata tggagaaacg gtagttgaaa cctgcttaca tcaagtctta
    12781 ttattagtaa gtagatacaa ttaaaactaa atttttctag aaaaaccttt ttagatttat
    12841 ctactctaat aaatatataa ttttttgtac tttataaata actataactt ctgctaaagt
    12901 aaagatgtat tgggagtaca tctttacttt cccattatag aagttaaaag catacaccat
    12961 aaaattaatc atccaataca aattatatat ttcctatgtc taacttaatt tacaagaatc
    13021 attctatatg catattctgc aatccactaa acatggctct aaacctttac tattttatac
    13081 attctctata aatcatacaa atcatatata aaatccccct aatatgttta aaccacctat
    13141 aaaataacac caatatttag aatattcaat atcaactgca taactggtca ataaaaacgt
    13201 ataactagac aaatttcaat atttatataa ataatacaag aaaaacaagc attttattta
    13261 tataagtttt ttattttttg tttatattaa aaaactatat tgat
//
LOCUS       DBGJFF010000106        13208 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.107, whole genome shotgun sequence.
ACCESSION   DBGJFF010000106 DBGJFF010000000
VERSION     DBGJFF010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13208
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.107"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(68..634)
                     /locus_tag="ACOIAF_002904"
     CDS             complement(68..634)
                     /locus_tag="ACOIAF_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3073 family putative ECF transporter S
                     component"
                     /protein_id="HGZ2669906.1"
                     /translation="MDTKKVTFSSMCIVINIVLGMVISMINIPLLFLDTVGTVLGAVV
                     LGPFWGALIGGCTNLVLGVISGPTNIPFALVNIVLGLIVGYISKKKGFGYKEAIITGV
                     ILSIVCPLIGTPISVLLFGGLSGSGADLLVGFLVQSGQKIFTAAFFPRILSNIVDKPL
                     SCIMVVYFINKMPKGFIKQYSKNTSSSY"
     gene            801..1322
                     /locus_tag="ACOIAF_002905"
     CDS             801..1322
                     /locus_tag="ACOIAF_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3072 family TudS-related putative desulfidase"
                     /protein_id="HGZ2669907.1"
                     /translation="MKRNREIVVVCHCILNCNSKVEGLSEFKGAQNIAIDLINKGYGI
                     IQLPCPEMIMYGINRWGHVKEQFDNLFFREQCRQMLIPHVRQFDEYLKNNYDIKSIIA
                     IDGSPSCGYNNTCSSKRWFGELSGCGELMDKINDIQMVEGKGIFIEELEKLLKEYNLE
                     IPIVGFDEIREDK"
     gene            1902..2723
                     /locus_tag="ACOIAF_002906"
     CDS             1902..2723
                     /locus_tag="ACOIAF_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HGZ2669908.1"
                     /translation="MNKQIKRLEEKSNQIRKEILDVVYNAKSGHVGGAMSSTDILTTL
                     YYHVMNISIENAKNSNRDRFILSKGHIAEALYCILADKGFFSRDKLKTYSQFNSTLIG
                     HPNNKNEGVEVSTGSLGHGLSLAVGMAIAGKRDKKSYRVFTLMGDGELAEGSVWEGAM
                     ASSHYKLDNLVAIIDRNKLQISGTTEEVMTLENLQEKWESFGWEVVQVDGNNIRELVE
                     VFDKIPLKKNKPTMIIANTIKGKGISFMENNVKWHHGVLTEEQYIKAVKDLKEGE"
     gene            2724..3665
                     /locus_tag="ACOIAF_002907"
     CDS             2724..3665
                     /locus_tag="ACOIAF_002907"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HGZ2669909.1"
                     /translation="MNNVANRKVICDVLVDIAKYDKDIMVLTSDSRGSASMSGFAELY
                     PEQFVEVGIAEQNLVGIASGLASCGKKTYVASPACFLSMRSIEQIKVDVAYSKNNVKL
                     IGISGGVSYGALGMTHHSLQDIAVTRAIPDIQVLLPADRYETKKMIQELYKSDIPAYI
                     RIGRNPVEDVYENEDFDFKIGRANELHSGNDITVIATGETVRIALDAAIELEKHGIEC
                     RVINMHTIKPLDKEAIIKSSKETKGIITIEEHSIYGGLGAAVSEVVVQNIPVKMKVLG
                     IADESPIAGNSKEVFNYYGLNSENIVKIAKEMLFSEN"
     gene            3677..5191
                     /gene="glpK"
                     /locus_tag="ACOIAF_002908"
     CDS             3677..5191
                     /gene="glpK"
                     /locus_tag="ACOIAF_002908"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: protein motif:HMM:NF000756.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HGZ2669910.1"
                     /translation="MDKKYILTIDQSTSGTKVLLIDSRGIIINKLSKKHTQYYLNNSW
                     IEHDPIEIYSNVICLLKEIIISHELCTGDIAGISITNQRETVVIWDKNTGKPVYNAIV
                     WQCRRTSDICKNLSVYKNEIQEKTGLKLDPYFSATKVKWILDNVDGARAKAERGDLLL
                     GNIDSWLVWNLTNGKSHATDYTNASRTLLLNIRDLKWDDSLLSIFDIPRSMLPKIYPS
                     DEIFGETNIEGYFSENLKISGVIGDSQGALFGQRCFEVGMAKSTFGTGNSIMINVGEN
                     FIPSQNGVVSTVGYITKNIVSYAVEGIVNSSGDTINWIKNELGLFSDFEEFNKAVEEV
                     KNNDGVYFIPAFIGIGVPFWKPNARASIVGISRNTTKKHIMRAAMESIAYQVKDAIDI
                     LEWESNITVKILSVDGGVTVNDIYNQFLADLLDCKIAKNKNEELSALGSAYLGGIGLG
                     VWNGIEEIKNLPENIKNYTSNVREENREKLYRKWKNAMKSVLYMTDLNSQNVNL"
     gene            5202..6626
                     /locus_tag="ACOIAF_002909"
     CDS             5202..6626
                     /locus_tag="ACOIAF_002909"
                     /inference="COORDINATES: protein motif:HMM:NF014649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose/L-arabinose isomerase family protein"
                     /protein_id="HGZ2669911.1"
                     /translation="MIEYKIKLGVAPTRRNIFSKKEAIRYKQLVSKKLKSMNIDFVDI
                     EDINEEGLLFKESDVEKVVDKFKQEKVDALFFPHCNFGTEDLVAKVAHKLHLPVLLWG
                     PRDGAPLEDGTRLRDSQCGLFATGKILRRFKVKFTYIPNASLECEAFERGVNNFIAAV
                     NVVKEIKNLRILQISTRPLSFWTMMCNEGELLEKFGVQVNPISLSEFVSCVKEVESKN
                     DEKLKETIQFLKENLEINVENESVVRVAALKLALNLLTEKYGCKAVTIQCWNALQEEL
                     NIMPCMANAILTDEGIPVVCETDIHGAITSVIAQSAAMGKTPPFFADWTVAHPTNKNG
                     ELLQHCGCYPISLTKEGEKAKFGVPFALESGCIGSSFAEIKGGNISLLRFDGDNGEYS
                     LLLGHAKGIEGPYIQGTYLWVEVENLTRLEDKLVTGPYVHHCVGIHHDVLPTIYEACK
                     YLPDLNLDFYDNVEEDVKAWIRGE"
     gene            6778..7803
                     /locus_tag="ACOIAF_002910"
     CDS             6778..7803
                     /locus_tag="ACOIAF_002910"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HGZ2669912.1"
                     /translation="MNSKTIMQDIAKLAGVSPGTVSNALNNRKGVGKETKERIIKIAE
                     EKGYFRNQKKNEDNAIRLIVFKKHGYVVSDTPFFSALIEGCERECRNNGYELLISHVR
                     DDVNSKENIDTIIRQKNVDGILLLATEMNEEDLRFFENLSISIPIVIVDSYHETKSSD
                     YVCINNVSGAYSAVKYFVEKGHKKIGYLGSSRSIKNFEYRATGFDKALNDLNIKQNQE
                     YKFLLEPTIDGAYNDMNKIIDEKVELPTALFAFNDIIALGAMKAMNEKGIKIPEDVSI
                     IGFDDITFSALANPALTTIKVQTVEMGKIAVNRLIDKIDNQINLKLKIEVETELIERD
                     SVKDINK"
     gene            7852..9129
                     /locus_tag="ACOIAF_002911"
     CDS             7852..9129
                     /locus_tag="ACOIAF_002911"
                     /inference="COORDINATES: protein motif:HMM:NF024602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4038 domain-containing protein"
                     /protein_id="HGZ2669913.1"
                     /translation="MSRLKVASDNRTFTLDKEHFFYLADTCWSAFTNIDFNEWRFYLE
                     KRKQQGFNTLQVNILPQWDRSAKHNELLPFDIKDDGAFDYSTLNKLYFENARKMCLLA
                     KEYGFQLALVVLWSNYVPGTWASAIMDKNIIPISFLSTYFDIVNKTFNDMNPIYLISG
                     DTDFEKEESIGYYDKALEFFNKVSPNTLKTLHIRGRLEEIPEILVSKIDFYMYQSGHN
                     SLFTNMPYYLAEVFYSKYPCKPILNSEPCYEQMGYSRKVYGRFNDYDIRKAGWQSVLS
                     GGCAGLTYGAHGIWSWHTNDSTFFMGIGEAFDSPLLWQSALQLPGAFDYGMIKYLLEQ
                     NNLKNIIPKNNLVVNDTDEIRYATNDDKSSVVLYIPINTNVKISENLSEYKFHLFDLE
                     TKNILIPLIEFKKDSTLFKMHSCNKDIVIIGQK"
     gene            9603..11459
                     /locus_tag="ACOIAF_002912"
     CDS             9603..11459
                     /locus_tag="ACOIAF_002912"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HGZ2669914.1"
                     /translation="MSTKYCDLSKEIIEGVGGLENISYVTHCATRLRFKLKNIGKANT
                     ENLKKNNGVITVVESGGQYQVVIGNHVSEVYNQLVKGTILETQVDNIEDKEKAKEKLI
                     DRFISAISAIFSPLMMALSGAGMLKALLILGNTVGVLQENSGAYMILYAAADAIFNFL
                     PIALTFTSAKYFKANQFLAFIIGASLVYPNIVALYNEGQSINFLGLPVILVSYTSSVI
                     PAIISVWALAKVEAFLNKIIPSILKSFLVPLLCISIIVPLTYLTIGPVTSYAGVYLAD
                     GYKWVMSINPIIAGGLVSVIWPTVIIFGLHWGFVPIVLNNIATYGRDTLFVITGPNNF
                     SQAGACLGVFLKTKDKDLKSIAGPAAVSAVLTGITEPAIYGVNLKFKKPFIIASIFSG
                     VAGAITAAAGTGVGAFIGTSLITLPAYIGVGFIGFLIACAIAYVGSAVTTFIWGFNDN
                     MILEGLTDKSVLDSKKDSVVLKSALQGELVSLKDIPDQCFSEGAMGQGIAIKPYKGEL
                     YAPADGEVKFVFPTKHALGFETNEGVEILMHIGIDTVELEGKYYEMHINAGDKVKAGD
                     LLVTFDIDKISEAGYSTITPIIVTNSSEFQDIIISTNDKELMIESDLLTVVI"
     gene            11577..12986
                     /locus_tag="ACOIAF_002913"
     CDS             11577..12986
                     /locus_tag="ACOIAF_002913"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015526340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HGZ2669915.1"
                     /translation="MSRFPENFLWGGAVAANQCEGAYNVDGKGLSIQDVTPNGIMTPR
                     TEKPTEDNMKLVGIDFYNRYKEDIKLFAEMGFKVFRTSIAWSRIYPKGDELEPNEKGL
                     KFYDDLFDECRKYDIEPLVTLSHYETPLYLAEKYDGWISRDLIDFYCRYVTTVFKRYK
                     DKVKYWLTFNEINSIVHEPFLSGGINTPKEKLTKNQIYQAIHHELVASAKAVKIGHEI
                     NPEFKIGCMVLSMPVYPLTPNPDDVICAMEKEHENLFFADIHVRGKYPSYSKRYFRKN
                     NIHIDVMDEDIEILKNTVDFISFSYYMSICESSDKTIKGSKGNIMGGAKNPTLKESEW
                     GWQIDPQGLRYVLNQFYDRYQKPLFIVENGFGAFDELIVGENGEFTVNDDYRIDYLRN
                     HLKQVAEAIDDGVDLMGYTTWGCIDLVSASTAELKKRYGFIYVDRNDDGSGTLNRYKK
                     KSFNWYKKVIETNGEYLFE"
BASE COUNT         5178 a         1387 c         2468 g         4175 t
ORIGIN      
        1 aaatgttata taaaaatgag tatatagcat tttaaagagt aataaacctt acataataaa
       61 tgatttatta atatgaacta gaagtatttt tactatattg ttttataaaa cctttaggca
      121 ttttatttat aaaatatacc accataatac aagacaaagg tttgtctact atattactaa
      181 gaatccttgg aaagaacgca gcagtaaata tcttctgacc agactgaact aaaaaaccaa
      241 ccaataaatc agcaccactt ccacttaaac caccaaacaa taaaactgaa ataggagtgc
      301 caattaaagg acaaacaata gataaaatta ctcctgtaat aattgcttct ttataaccaa
      361 aacctttttt cttagaaata tatccaacaa taagaccaag aacaatattt acaagggcaa
      421 aaggtatatt tgtaggtcct gatataacac ctaaaactaa atttgtacac ccgccaataa
      481 gagcacccca aaaaggacct aacacaacag ccccaagtac agtaccaact gtatctaaaa
      541 ataataatgg tatatttatc atagatataa ccatacctaa gacaatatta ataactatgc
      601 acattgaaga aaatgtgact tttttagtat ccaaatataa tacctcctaa aaaaatttat
      661 acatttatca tagtatagat tataaaagat tactaattgt atatttctat atagaatatt
      721 ggaaatatag aacaaataaa taatataatt atcaaaatat atagtatcat taaaattaag
      781 aataactaag gagattatgt atgaagagaa atagagagat tgtagtagta tgtcattgta
      841 tattaaattg taattctaaa gtagaggggt tgtcagaatt taaaggagca caaaatatag
      901 caatagattt aataaataaa gggtatggaa taattcaact accatgtcca gaaatgatta
      961 tgtatggaat aaataggtgg ggacatgtaa aagaacagtt tgacaatttg ttttttagag
     1021 aacaatgtag gcaaatgtta atacctcatg taaggcagtt tgatgaatat ttaaaaaata
     1081 attatgatat aaaaagtatc attgcaatag atggtagccc aagttgtgga tataataata
     1141 catgtagttc taaacgttgg tttggtgagt taagtggttg tggtgaattg atggataaaa
     1201 taaatgatat acaaatggtg gaagggaaag gtatttttat agaagagcta gaaaagctac
     1261 ttaaagagta taatttagag atacctatag ttggatttga tgagattaga gaagataagt
     1321 aaatgtataa atatttatca aaaagatgac attataaaat gttcaaaaaa actttaagaa
     1381 ttaaagaaat aaatatacaa aatgtataac taagtttaca ggttaaaaag aaagctgttg
     1441 tgtaaataat ataagaaaaa tcttacactt ttttgtaaaa attgaaaata tcaatctaat
     1501 gatgataaaa taagagtaat taaaataaat gtaatattaa aatttgtgag aaagagtata
     1561 tttcaaaata gagataagac tctttttcgt tgttaaactg atgaaaatat atttgatttc
     1621 atataaaaaa gagtgcctca tgaactaaaa agttcacttt gagacactca tttttttgtt
     1681 tatataccat aggttttatt tataaccttg gatatacgat gtaattttaa tttacagtgt
     1741 ttaacttcta atgactaaat atttagcaat acaaagaagc aaaaaactaa aacataatca
     1801 atattaataa taaatattta ctaatttaaa aataagtgat aatataaaaa tataactaaa
     1861 tatttactaa atataaaatg aagatttata tggaggggtc tatgaataaa cagattaaaa
     1921 ggttagaaga aaaatcaaat caaataagaa aagaaatatt agatgttgta tataatgcaa
     1981 aatcaggaca cgtaggaggt gcaatgtcta gtactgatat cttaacaact ctttattatc
     2041 atgtaatgaa tataagcatt gaaaacgcaa aaaactcaaa tagagataga ttcatattaa
     2101 gtaaaggaca catagcagaa gctctatact gtatattagc tgataaagga ttttttagta
     2161 gagataagtt aaaaacatat agccaattta attcaacact tataggtcat ccaaacaata
     2221 aaaatgaagg ggtagaggtt agcacaggtt ctttaggtca tggtctttca ttagctgttg
     2281 gtatggccat agcaggaaag cgtgataaaa aaagctatag agtttttaca cttatgggag
     2341 atggtgaatt agctgaaggg tctgtatggg aaggagctat ggcatctagc cattacaagt
     2401 tagataacct agtagcgatt atagatagaa ataaattgca aatatcagga actacagaag
     2461 aagttatgac acttgaaaat cttcaagaaa agtgggagag ctttggctgg gaagtagttc
     2521 aggtagatgg gaataatata agagaattgg ttgaggtctt tgataagata ccattgaaaa
     2581 aaaataagcc aacgatgata atagctaata ctataaaggg caagggaata tcttttatgg
     2641 aaaataacgt aaaatggcat catggagtgc taacagagga gcaatacata aaagcagtta
     2701 aagatttaaa ggagggagaa tagatgaata atgtagcaaa ccgtaaagta atttgtgatg
     2761 ttttagtgga tatagcaaaa tatgataaag atataatggt tttaactagt gattctagag
     2821 gctctgcctc aatgtcaggg tttgctgagt tatacccaga acagtttgtt gaagttggaa
     2881 tagccgagca aaatttagtt ggtattgcat cgggattagc atcttgtggc aaaaaaactt
     2941 atgtagcttc tccagcatgt tttttatcaa tgagaagtat agagcaaata aaagtagatg
     3001 tagcttattc taaaaataat gtcaaactta taggtataag tgggggtgta agttatgggg
     3061 ctctaggaat gactcatcat tcccttcaag atatagcagt aactagagca ataccagata
     3121 tacaagtact gcttccggct gaccgatatg agactaagaa gatgatacaa gaattgtata
     3181 aaagtgatat tccagcttat ataagaatag gaagaaatcc tgttgaagat gtatatgaaa
     3241 atgaagattt cgactttaaa ataggtagag ctaatgaatt acatagtgga aatgatataa
     3301 cagtgatagc aactggagaa acagtaagaa ttgcactaga tgcagctata gaacttgaaa
     3361 aacatggtat tgagtgtaga gtaataaata tgcatacaat taagccttta gataaagaag
     3421 ctataataaa atcttctaaa gaaacgaagg gaattataac aatagaagaa cacagtatat
     3481 atggtggatt gggagcagca gtatcagagg tcgtagtcca aaatatccct gttaagatga
     3541 aagtgttagg aatagcagat gaatctccta tagctggtaa tagtaaagaa gtatttaatt
     3601 attatggact gaatagcgaa aatatagtga aaatagctaa ggaaatgcta tttagtgaaa
     3661 attaaagagg caaaatatgg ataaaaaata tatattaacc atagaccaga gtacatcagg
     3721 aacaaaggta ctgcttatag atagtagagg aataataata aataaattat ctaagaaaca
     3781 tacccaatat tatcttaata atagttggat agaacacgac cctattgaaa tatacagtaa
     3841 tgtaatctgt ttattaaagg aaataatcat atctcatgaa ttatgtacag gggatatagc
     3901 tggtatttct ataacaaatc aaagagagac tgtagtaatt tgggataaga atacaggtaa
     3961 gcctgtatat aacgccatag tatggcaatg tagaagaact agtgatatat gcaaaaatct
     4021 aagtgtatat aaaaatgaaa tacaagaaaa gactggttta aaattagacc cttatttttc
     4081 agctactaaa gtcaagtgga tattagacaa tgtagatggc gctagagcta aagctgaacg
     4141 aggtgattta ttacttggta atattgattc atggttagtt tggaatctta ctaatgggaa
     4201 aagtcatgct acagactata ctaacgctag tagaacctta ttgcttaata taagagattt
     4261 gaagtgggac gattctttat tgagcatatt tgatatacca aggagtatgt tacccaaaat
     4321 atatccatca gatgaaatat ttggagaaac taatattgaa ggatattttt cagaaaatct
     4381 taagattagt ggagtaattg gagattctca aggtgctttg tttggtcaaa gatgttttga
     4441 agtaggtatg gcaaagtcta catttggtac tggtaattct ataatgataa atgtaggaga
     4501 gaactttatt ccttctcaaa atggagttgt atcgactgtt ggttatatta ctaaaaatat
     4561 agttagctat gcagttgaag gaattgtaaa ttctagtgga gatacaataa actggataaa
     4621 aaatgaatta ggtctatttt ctgattttga agaatttaac aaggctgtcg aagaagttaa
     4681 aaataatgat ggtgtatatt ttataccagc atttatagga attggtgtac ctttttggaa
     4741 gcccaatgcg agggcatcta tagtaggtat atccagaaat actactaaaa agcatataat
     4801 gagagctgct atggaatcaa tagcatatca agttaaagat gctattgata tattagaatg
     4861 ggaaagtaat ataacagtta aaattttaag tgttgatggt ggtgtaacgg taaatgatat
     4921 atataatcaa tttttagcag atttacttga ttgcaaaatc gctaaaaata aaaatgaaga
     4981 gttatcagct ttaggttctg catacttagg aggtataggt ttaggtgttt ggaatgggat
     5041 agaagaaatt aaaaatttac ctgaaaatat aaaaaactat acttctaatg taagagaaga
     5101 aaatagagaa aaattgtata gaaaatggaa aaatgcaatg aaaagtgtat tatatatgac
     5161 agatttaaat agccaaaatg ttaatttata ggagagataa tatgatagag tataaaataa
     5221 agctaggagt tgcaccaact cgtagaaata tatttagtaa gaaagaagct ataaggtata
     5281 aacaattagt aagtaagaaa ttaaaatcca tgaatataga ttttgttgat atagaggata
     5341 taaatgaaga aggactgtta tttaaagaat cagatgtaga gaaggttgtc gataagttta
     5401 aacaggagaa agtagatgct ttgttttttc cacattgtaa ctttggtact gaagatttgg
     5461 tggcaaaagt tgctcataaa ttacatctac cagttctttt atggggacct agagatggag
     5521 ctccactaga agatggaaca agacttagag atagtcaatg tggtctattt gctacaggta
     5581 agatacttag aagatttaaa gttaagttta catacatacc aaatgcatct ctggaatgtg
     5641 aagcttttga aagaggtgta aataatttta ttgccgcagt taatgtagta aaagaaatta
     5701 aaaatttaag aatacttcaa atatcaacta gaccattatc tttttggact atgatgtgta
     5761 atgaaggtga attacttgag aagtttggag ttcaagtaaa tccaatttct ttatcggaat
     5821 ttgttagctg tgtaaaagaa gtagaatcta aaaatgatga aaaattaaag gaaacaatac
     5881 agttcctaaa agagaattta gaaataaatg ttgaaaatga gagtgtagtt agggtcgcag
     5941 cacttaagtt ggcacttaac cttttaactg aaaagtatgg atgtaaagca gtaactattc
     6001 aatgctggaa tgccttacaa gaggagctta atataatgcc atgtatggca aatgctattc
     6061 ttactgatga ggggatacca gtagtttgtg aaactgatat acatggagcg ataacctctg
     6121 tcatagctca atctgctgca atggggaaaa caccaccgtt ttttgctgat tggacagtag
     6181 cacatccaac caataaaaat ggagaattgc ttcaacattg tggatgttac cctatatctt
     6241 tgactaaaga aggagaaaaa gcaaaatttg gagttccttt tgctcttgaa agtggatgta
     6301 taggttcatc atttgcagaa ataaaaggag ggaatatatc tttattaagg tttgatggag
     6361 ataatggaga gtattctttg ttgttaggac atgcaaaagg aatagaagga ccttatatac
     6421 aaggaacata tctttgggtt gaagtagaaa acttgacaag attagaggat aagcttgtaa
     6481 ctggtccata tgtgcatcat tgtgttggaa tacatcatga tgtactacct actatttatg
     6541 aagcttgtaa gtatttacca gatttaaatc tagattttta tgataatgta gaagaggatg
     6601 taaaggcgtg gataagagga gaataagatt ttgtttatta aaaattgaaa agcattagta
     6661 aaataaaaat atttactaaa tatttagctt aagtgtttat tttatttaat tattgcctta
     6721 tactataatt tatggagtgt gaatttaatc atttaatata gaaaaggtag tgataaaatg
     6781 aatagtaaaa ctataatgca agatatagct aaattagcag gtgtatcgcc tggaactgtt
     6841 tcaaatgcat tgaataatag aaaaggtgta ggaaaagaaa ctaaagaaag aataattaaa
     6901 atagcagagg aaaaaggtta ttttagaaat cagaaaaaaa atgaggataa tgcaattaga
     6961 ctaatagtat ttaaaaagca tggctatgtt gtttctgata ctcctttttt ctctgcgctt
     7021 atagaaggtt gtgaaagaga gtgtcgtaat aatggttatg aactcttgat ttctcatgtt
     7081 agagatgatg ttaattcaaa agagaatatt gatacaataa taagacaaaa aaatgtagat
     7141 ggtattttat tgttggcaac agaaatgaat gaagaggatt taagattttt tgaaaattta
     7201 agtatatcta tacctatcgt tatagtagat agttaccacg aaactaaaag ttcagactat
     7261 gtttgtataa acaatgtaag tggtgcatac tctgctgtaa agtattttgt tgaaaagggg
     7321 cataaaaaaa taggttactt aggaagttct agaagtataa agaattttga atatagagca
     7381 actggatttg ataaggctct aaatgattta aatataaaac aaaatcaaga atataagttt
     7441 ttattagagc ctactataga tggtgcttat aatgatatga acaagataat agatgaaaaa
     7501 gtagagctac caactgcatt atttgcattc aatgacataa ttgctttggg agcaatgaaa
     7561 gcaatgaatg aaaaaggaat caaaattcct gaagatgtat ctataatagg atttgatgat
     7621 ataactttta gtgcacttgc taatcctgct ttaacaacta ttaaggtaca gactgtagaa
     7681 atggggaaaa tagctgtaaa taggcttata gataagattg ataatcaaat taatctgaag
     7741 ttaaagatag aagtagaaac agaattaata gaaagagata gtgttaaaga tattaataag
     7801 taatagtttt aatcttgttt aagactattg ttaaggaaga gggttgtaat tgtgagtaga
     7861 ttaaaagtag catctgacaa tagaactttt actttggata aagagcattt tttttattta
     7921 gcagatacat gttggagtgc atttacaaat atcgatttta atgagtggag gttttatctt
     7981 gaaaagagga agcaacaagg atttaatact cttcaagtaa atatacttcc tcaatgggat
     8041 agaagtgcta agcataatga gttgttacca tttgatataa aggatgatgg agctttcgac
     8101 tattcaactt tgaataaact atattttgaa aatgctagaa agatgtgttt attagctaaa
     8161 gaatatgggt ttcaattagc tttagtggta ttgtggtcaa attatgtacc aggaacatgg
     8221 gcaagtgcta ttatggataa aaatataata cctatatcgt ttttatcaac ttattttgac
     8281 atagtaaata aaacttttaa tgatatgaac cctatttatt taattagtgg ggatactgat
     8341 tttgaaaaag aagaaagtat aggatattat gataaagcat tagagttttt taataaagta
     8401 tctccaaata cattaaaaac tctccatatc agagggaggc tggaggaaat accagagata
     8461 ttagtaagta aaattgattt ctatatgtat caatcaggac ataatagttt atttaccaat
     8521 atgccttact acttagcaga agtattttat agtaaatatc catgtaaacc aatcttaaat
     8581 tcagaacctt gttatgagca gatgggctat agtagaaagg tgtatggtag atttaatgac
     8641 tatgatataa gaaaggcagg atggcaaagt gtactatctg gaggttgtgc tggattaacg
     8701 tatggagctc atgggatatg gagttggcac actaatgatt cgacattttt tatgggaata
     8761 ggagaagctt ttgacagtcc tttattgtgg caaagtgcac ttcaattacc aggtgctttt
     8821 gattatggca tgataaagta tcttcttgaa caaaataatt taaaaaatat tatccctaaa
     8881 aataatcttg ttgttaatga tacagatgaa ataagatatg caactaatga tgataaatca
     8941 tctgttgtac tatacatacc gataaatact aatgttaaaa taagtgaaaa tttatcagag
     9001 tataaatttc atttgtttga tttagaaaca aagaatattc ttatcccttt aattgaattt
     9061 aaaaaagata gtactttatt taagatgcac tcatgtaata aagatatagt aattatagga
     9121 caaaaataaa tattgtaata aattttcaaa atcctaagca tatgaaatat tgcttaggat
     9181 ttttgtgttc aataaaaaaa tgaaaataat tagataaata tttagtttaa aaataataaa
     9241 tatttacaaa aacaattagt tatgctacta taaaaatata aagaatattt agacttagaa
     9301 aggagtgaag atactaatgt tctaacaagt gatgttaact tcttaagaca tgatttgatt
     9361 agatttaacg aggattaata gtttgaaaat tatggaaaaa gtttacagaa gtaaatattt
     9421 agtattaatt aatatacttg aactaaatga tataaaaata taagatacat agaatgttta
     9481 taattaattt gaaatcgttt tctaaaatat cagtaagtaa ttcaaaacat gtaaaagaga
     9541 agttgatata gttgattatt tgagataaaa attgatttta tattaaatgt gggaggttta
     9601 ttatgtctac aaaatattgt gacctatcta aagagataat agaaggagtt ggtggattag
     9661 aaaacatatc atatgttact cactgtgcaa ctagattaag gtttaagtta aaaaatatag
     9721 gaaaagcaaa tacagagaat ctaaaaaaaa ataatggtgt aataacagta gtagaaagtg
     9781 gaggtcagta tcaagtagtc ataggaaatc atgtttctga agtatacaat caactcgtaa
     9841 aagggactat acttgaaacg caagttgata atatagaaga taaagagaaa gcaaaagaaa
     9901 aacttattga tagattcata tctgcgatat ctgcaatttt ttctccttta atgatggctc
     9961 tttcaggtgc gggtatgtta aaagctttat taatactagg aaatacagta ggagttttac
    10021 aagagaatag cggtgcatat atgattttat atgcagcagc agatgcaatt tttaattttt
    10081 tacctatagc tttaacattt acatcagcca aatattttaa ggcaaatcaa tttttagcat
    10141 ttatcattgg agcatcgtta gtatatccaa atatagttgc tttatataat gaaggtcaaa
    10201 gtataaattt tttaggatta ccagttattc tagtaagtta tacttctagt gtcataccgg
    10261 caattattag tgtatgggca cttgctaagg tagaagcatt tttaaataaa ataataccaa
    10321 gtatattaaa aagtttttta gtcccactat tatgtatatc gattattgtt ccattaacat
    10381 atttaactat tggaccagtt acaagctatg caggagtata tttagctgat ggatataagt
    10441 gggttatgag tataaatcct ataattgcag gaggtttagt atctgtaata tggccaacag
    10501 taataatatt tggattgcat tggggatttg tacctatagt attaaataat atagctacat
    10561 atggtagaga tactttattt gtaataactg gacctaataa ttttagtcaa gctggagctt
    10621 gtttgggagt gtttttaaaa acaaaagata aagatttaaa atctatagca ggacctgcag
    10681 cagtttcggc agtattaaca ggaataacag agcctgcaat atatggagtc aatttaaagt
    10741 ttaaaaagcc ttttattatt gcaagtatat tttctggagt agcaggtgct ataacagcag
    10801 cggcaggcac aggtgtagga gcatttattg ggacaagttt aattacttta cctgcatata
    10861 taggtgttgg ttttattggt ttcttaatag catgtgcgat tgcttatgta gggtcagctg
    10921 ttacaacttt tatatgggga tttaatgata acatgatttt agaaggactt acagataaaa
    10981 gtgttttaga ttcaaaaaaa gatagtgtag tgttaaagag tgcattacaa ggagaattgg
    11041 tatctttaaa agatatacct gaccagtgtt tttcagaagg agctatgggg caaggaattg
    11101 caataaaacc atataaaggt gaattatatg cacctgcaga tggagaagta aaatttgttt
    11161 ttcctacaaa acatgcttta ggatttgaaa ctaatgaagg tgtagaaata ttaatgcaca
    11221 taggaattga tacagttgaa ttggaaggaa aatactacga aatgcacatt aacgctggag
    11281 ataaagttaa ggctggagat ttgttagtta cgtttgatat tgataaaatc agtgaagctg
    11341 ggtattcaac aataacacca attattgtga ctaactcatc agaatttcaa gatataataa
    11401 taagtactaa tgataaggag ttgatgatag aatctgattt attaacagta gtaatataaa
    11461 tttgattaac tagaaagtga attattgtac atatattaat aaatataaca taagaaattt
    11521 cttaaagatt atattttaag taacgtttaa tattgaatta agggagatga tagaaaatga
    11581 gtagatttcc agaaaatttt ttatggggtg gtgcagtagc tgcaaaccaa tgtgaaggcg
    11641 catataatgt agatgggaaa ggacttagca tacaggatgt gactccaaat ggaattatga
    11701 ctccaagaac tgaaaagcca acagaagata atatgaaatt agttggaatt gatttttata
    11761 atagatacaa agaagacatt aagttgtttg ctgaaatggg atttaaagta tttagaacat
    11821 caatagcatg gtctagaatt tatcctaagg gagatgaact agagccaaat gaaaaaggtc
    11881 ttaaatttta tgatgaccta tttgatgagt gtagaaaata tgatatagaa ccattagtta
    11941 ctttgtctca ctatgaaaca ccattatatt tggcagaaaa gtatgatgga tggataagtc
    12001 gtgatttaat agatttttat tgtaggtatg taactacagt ttttaaaaga tacaaagata
    12061 aggtaaagta ctggcttaca ttcaatgaaa taaactctat agtacatgaa ccatttttaa
    12121 gtgggggaat taatactcct aaagaaaagt taacaaaaaa tcaaatatat caagctatac
    12181 accatgaatt ggtagcaagt gcaaaagcag taaagatagg gcatgagata aatcctgagt
    12241 ttaagatagg atgtatggtg ttatctatgc cagtttatcc attaactcca aatcctgatg
    12301 atgtgatttg tgcaatggaa aaggaacacg aaaatttatt ctttgcagat attcatgtta
    12361 gaggtaaata cccaagttat tcaaaacgtt attttagaaa aaataatata catatagatg
    12421 taatggatga agatattgag atattaaaaa atacagtaga ttttatatct tttagttatt
    12481 atatgagtat ttgtgaaagt tcagataaga ctattaaagg tagtaaaggc aatatcatgg
    12541 gtggagcaaa aaatccaaca ttaaaagaaa gtgaatgggg atggcaaata gacccacagg
    12601 gtcttagata tgtacttaat caattctatg atagatatca aaaaccattg tttatagtag
    12661 aaaatggatt tggagctttt gatgaattaa tagtgggtga aaatggagag tttacagtta
    12721 atgatgatta tagaatagac tatttgagaa atcacttaaa gcaagttgct gaagctattg
    12781 atgatggtgt tgatttgatg ggatatacta catggggatg tatagattta gtaagtgcgt
    12841 caacagcaga gcttaagaaa cgttatggat ttatttatgt agaccgtaat gatgatggaa
    12901 gtggtacttt aaatagatat aaaaagaaga gctttaattg gtataagaag gttatagaaa
    12961 caaatggaga atatttattt gaatagatat acaaaattta gtgaaattga atatttagga
    13021 taaaaaagga ggggatgttt caaaattaat tttttctaat aaaatcttaa attcaccccc
    13081 ttttttaatt tttatatgtt attttatttt tcattatttc taaatccttt acttattgaa
    13141 atttactact atcaatcata ttatttcatg actttcaacc gtgggtaata tataattaca
    13201 catattga
//
LOCUS       DBGJFF010000107        13115 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.108, whole genome shotgun sequence.
ACCESSION   DBGJFF010000107 DBGJFF010000000
VERSION     DBGJFF010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13115
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.108"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            370..1074
                     /locus_tag="ACOIAF_002914"
     CDS             370..1074
                     /locus_tag="ACOIAF_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGZ2669916.1"
                     /translation="MSKLIYIADDEDNIRNLVKTFLKNEGHDVMDFKTGDELLEQFNI
                     KECDLVILDIMMPGSSGFEVCTKLREKSTVPIIMLTARDTDIDYITGITLGSDDYFTK
                     PFSPMSLVMRVKSIFRRIEFEKKQNYDKYSNSIDMELKFGDVIINKKNKIVTSKNVNI
                     DLTPNEYNLLTYLFENIDRAVSRDELLNKIWGYDIEVETRAADDTVKRLRKKILNTNI
                     LIETVWGFGFRLKEKS"
     gene            1076..2563
                     /locus_tag="ACOIAF_002915"
     CDS             1076..2563
                     /locus_tag="ACOIAF_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HGZ2669917.1"
                     /translation="MKNGNDKNRIGKIKSKFKLKFSKKNISIKTRILLSILCLIIIVF
                     MIILVSFNVFLDTYIKTTANEELTKSTEIVEHMDSNFRPIKPPQDDGKLPPKVLSNFM
                     RNVQDKVRMAETQSDADAMVVDSKYNLIFPTREDDFLKNVDEMELIRECIQNEKLDLN
                     SDANTRISTGNKDYYISTVKITSIYGEQDKYLILFIDISKTLNLAQKINIVLISVMCF
                     AGILAIFTAVILSEKIAKPIKELCEFAKKMGQGDFKRTYFDFSDKELVELSTVMNKSA
                     EYLDKYDNEQKIFFQNASHELRTPLMSIKGYAEAIKYNVIDSKHASDIILEESDRLSD
                     MVEDLLYISKIDNITKDFELVECDLREVLSNCGARQNVRAINKGIKFIYEFDEEMVLF
                     ECDEKNISKAFMNLIENALRYAKSEIKIVCKYNQKNIVVIIEDDGIGIKKEDLPHVFE
                     RFYKGVGGNHGIGLSIVKSIVNKHGGRIYVENGKKGAKFTILFKL"
     gene            complement(2674..3237)
                     /locus_tag="ACOIAF_002916"
     CDS             complement(2674..3237)
                     /locus_tag="ACOIAF_002916"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669918.1"
                     /translation="MGISKCTSFLNRQIILQVNKKTILDKIVANTLILLFSSIISKPS
                     FTNKTKILVTTISDSIKNILNKTAVNNRVFKFPLYKNDVEKIRMKIKGIVILGFKVIK
                     LQNPKHIIKPSLAYLTTFIYPSFWPKKSDKNIWMTKASNTTVTKFVILKISCSFIHIF
                     YKIRSLLKPKYTSDFTFPFVSIFKHLL"
     gene            3230..4381
                     /locus_tag="ACOIAF_002917"
     CDS             3230..4381
                     /locus_tag="ACOIAF_002917"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HGZ2669919.1"
                     /translation="MPIKYWLPLFLIPIFSLFLSVSIFDVSKFFSLESLKFLSLISSV
                     GILYINFIVFYLFKFIIDKTKLSMKYELLEKEIIHKEELRLSNECYKIIVEQTDSVVF
                     EWNIKENKSFVSQAWTEKFGYNNACKNIFKEIKDKDLVHSEDKAIFEGFLESIKKKNM
                     HNQAVYRLKKSNGEYIWCRTSITSIYNDENELLRVVGVIVDVDSDIKKYEELRTRAES
                     DSLTNIYNKGTFEKLVEETIVMNTGDKKDALFIIDLDDFKEINDNFGHPFGDFVLKTF
                     ADKIQTSFGSKDLVGRIGGDEFVVYMQDYVTEVNLHKKAKELNRVLSDNYTDLSFSFD
                     ASVSIGIARYPQDGTSFFELFKNADRALYSIKASGKNSYCLFEEELYVQ"
     gene            complement(4743..5573)
                     /locus_tag="ACOIAF_002918"
     CDS             complement(4743..5573)
                     /locus_tag="ACOIAF_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HGZ2669920.1"
                     /translation="MSQVSKNYFTTGEFAKICGINKKTLFHYDDIGLFSPELKKENGY
                     RYYSYHQLSIFGIISSLREVKMPLKEIKAYIDKRTPNLLIELLEKKTIDIKNEIEKLN
                     NIQALMESTISFTKNACNIDANTITLKEHEEEYLVKTPVTYKEQFLGDEEENFLYECI
                     NFMDNYELSDYGTIGSIIKGEDIINKDFESYSYLFTKVNKEYKKYPVSIRPKGLYVTA
                     YHKGSYETIYKTYEKLLNFFNQNNLRIGDFVYEEYLLYDISVRDSNEYLTQISAEVKM
                     "
     gene            5646..7373
                     /locus_tag="ACOIAF_002919"
     CDS             5646..7373
                     /locus_tag="ACOIAF_002919"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2669921.1"
                     /translation="MKKLIHFLKPYRVLIVVVLIFTFLQTLGTLYIPTLTANIVNNGV
                     VKGDIDYIVKTGLMMMIVAGITALSAVLVCKVSTNLSSGFCRDIREAVFIKSQDLSIN
                     DFNNIGTASMITRSTSDITLIGQSVFMFIQLVLPAPIITVSGLFLAYSIDKAMTIIIV
                     VVMFLFMLSAFLVGKKLIKLFKMMQIKMDNMNRVLREVVTGVRVIRAFNRSRFEKKRF
                     DRTAIDYSETAISINKIFAVLMPIVMLIMNLGIVSIIWFGGMRVSNGNMEIGHIMALV
                     EYCILILFYLIMGVMVFMYIPRAGACADRVNQILDTEPEIVDGNGHKDTVSERGHLVF
                     KNVTFSYAQSEEPVLNNITFEAKSGEVTAIIGSTGSGKSTIANIIPRFFEIQSGEISI
                     NGQDIKKIPQKELRDKIGFVPQKAFLFSGTIEENIRYGKEDASIEEVKHAASIAQADE
                     FISDMEDKYDSFVAQGGNNLSGGQKQRISIARALVRKPEVYVFDDSFSALDFKTDKRL
                     RKALKNEIKDSSAIIIAQRISTIMDANQIIVLNDGKIVGIGKHKDLLENCEVYKQIAD
                     SQLSKEELA"
     gene            7410..9272
                     /locus_tag="ACOIAF_002920"
     CDS             7410..9272
                     /locus_tag="ACOIAF_002920"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2669922.1"
                     /translation="MSEKRIDEQDIEVLSFEDGMSAEKAKDAKKTTKRLLKYMAKQKF
                     KILIIFISVLISSALTVLAPMVMGKAIDQLFNGIKTAVQTGTKFSVNFSTMGGIVSIL
                     LGLYLISAVFIYIQQYIMSGVAQNLVLSMRKDLSDKLNKLPLKYYDSHKKGETLSIVT
                     NDLEKVADSLQEGLMQLITAVVTVVGSIVMMISISVPLTIVSAITLLVSLGITVVIAR
                     KSQERFSENQKALGELNSNIEEIFTGQIVVKAFSKEKDTIRNFKILNQNLYNASRKAQ
                     FSSYAISPIIRFINLIGYVIIAVVGGIFASTGAMTLGSIQAFIQYVNQASEPTTEISY
                     IVNMLQAAIASAERVFTVMDEVEEIKDEESSKVISMPKGKVQFEHVKFGYSDDFILMK
                     DININLNAGDKIAIVGPTGAGKTTLVNLLMRFYEIQGGRITIDGVNIKDLKRGELRTM
                     FGMVLQDTWLFNGSIKENIAYSKSDATMDEIVRAAKSARVDHFIRTLPQGYDTILTED
                     ASNLSQGQKQLLTIARAILSDPSVLILDEATSSVDTRTEVEIQKAMNNLMKGRTSFVI
                     AHRLSTIRDADLILVMKEGTIIEQGSHKELIEKKGFYEELYNSQFTSEYDEDVV"
     gene            9605..10294
                     /locus_tag="ACOIAF_002921"
     CDS             9605..10294
                     /locus_tag="ACOIAF_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HGZ2669923.1"
                     /translation="MNKKLNYRFEMKHKITEADVLALKSRLYPIMKKDENASKDGKYL
                     IRSLYFDTPEDKALLDKLNGVAIREKFRIRFYNNDCSYIRLEKKIKHYNMTSKLSTNL
                     TKKEVINILNNDIEFLKESTNCLLREFYLKLKCERLEAKSIVDYNREAFIYPVGNVRV
                     TIDSDIKTSVNSVDLFNKDLPTISVIDENMTVLEVKYDEFIPDFIKDLIQINKTTSTA
                     VSKYASSRLYI"
     gene            10411..11085
                     /locus_tag="ACOIAF_002922"
     CDS             10411..11085
                     /locus_tag="ACOIAF_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HGZ2669924.1"
                     /translation="MTFNDIFKSSFIENVSGFSFVDSALALGSAFLVGLFIYMVYKKT
                     YMGIMYSRPFNVSLVALTMLTTFVILAVTSNVVLSLGMVGALSIVRFRTAIKDPMDLV
                     FLFWSLGSGIVLGAGLIPLAVMGSIIMGLILIFFSNKTISETPYILMVNCNNEDSEDI
                     ATDKIKKVFTKYQIKSKSVTPEKGIELVFEVRMKDGETSLINDLSQVDGVTNAVLVSY
                     NGDYVA"
     gene            11102..13009
                     /locus_tag="ACOIAF_002923"
     CDS             11102..13009
                     /locus_tag="ACOIAF_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HGZ2669925.1"
                     /translation="MKDKKFTLLISIMIIFLCAVVGVYSTSSNKSADLYSDVYIEKYF
                     NRDKVMEVNIEIDESDLKDMNENAIKEEFKVAKVTVDGDTYGNVGIRTKGNSSLISVA
                     NSDSDRYSYKINFDKYNTSQSMEGLTQLNLNNCYSDPSYMREFLTYSICEEMGLATPE
                     FAYAKVSINGEYHGLYLAVEGLKESYLENNFGNVTGDLYKSDEGSSLQYKGDDPESYS
                     NLIVESDKKTADWSKITKLLKSLDTGEDIEKYLDVDSVLKNIAINTALLNLDSYQGSF
                     AHNYYLYEQDGVFSMLPWDFNMSFGGFSGFGGGSQSIAIDEPTTGNLEDRPLISSLLK
                     NETYKTKYHKYLEEIVTKYLDSDYLENMTTKLHDMIASYVKEDPTAFYTYEEFEKNIT
                     SSIEDSSDNKGFGNKGFDNNNSNNSDSNNNSNSENKRSGNQSDEKEVNAELTSSVVKA
                     NTDNETKNKTTNDSESKNNTDKDKSGNDNNQKLEGPMGKGGKSIPGVLEVAEDMSKTI
                     KSQLSGETSSTKQNSGDESSSGIKGSEKFDEDMSGMPEPPEGMDGKMPPGMGNMDKGD
                     MNGKNGNMNMDRNQDNPREAGGFGNRGGGSVSKTTTYFKLILGGASMIIMSIMLVGVS
                     RVKRRRFIKSK"
BASE COUNT         5101 a         1357 c         2270 g         4387 t
ORIGIN      
        1 caataaaaag tttgaaaaat atcaattttg atataaactg aataaataca gttgaatatt
       61 aaattattat tttatcaaaa atatgcaaag tactacacct tcaaatatca cctaaagtaa
      121 ttaaaaggca attaaagtga agtagttcaa gtatttctaa aataagttta ataaattcta
      181 taataattta ttttttgcct taaaattgcc ttaaaatcca aacaatttta aaactaatgt
      241 actataaaaa gctggtataa tttaaatata gtaaaaaata agatttatct tatctattgg
      301 gatttaatta tttaatcaat atatttatac catttaattt aaataaaaag tctattaagg
      361 agtgagctta tgagtaagtt aatttatata gcagatgatg aagataatat aagaaatttg
      421 gtaaagacat tcttaaaaaa tgaaggtcat gatgttatgg attttaaaac gggtgatgaa
      481 cttttagaac aatttaatat aaaggagtgt gacttagtaa tactagatat aatgatgcct
      541 ggctcaagtg gttttgaagt atgtacaaaa cttcgtgaga aaagtacagt cccaataatc
      601 atgcttactg caagagatac agatatagat tatattacag ggataacctt aggtagtgat
      661 gattatttta caaaaccatt tagcccaatg tctttggtta tgagagtaaa atctatattt
      721 agaaggatag agtttgaaaa gaaacaaaat tatgataaat atagtaactc gatagatatg
      781 gaattaaaat ttggtgatgt aataataaat aaaaagaata aaatcgttac aagtaaaaat
      841 gtaaatatag accttacacc aaatgaatat aacctattga cttatttgtt tgaaaatata
      901 gatagagcag tttccagaga tgaattatta aacaaaatat ggggatacga tatagaagtt
      961 gagacaaggg ctgctgatga tacagtgaag agattacgta aaaaaatttt aaatacaaat
     1021 attttaatag aaactgtatg ggggtttgga tttaggttga aggagaaaag ttaagatgaa
     1081 gaatggaaat gacaagaata gaattggaaa gattaaatcc aaatttaaat taaagttctc
     1141 aaagaaaaat ataagcataa aaactaggat tttgttgagt atactttgtt tgataattat
     1201 agtatttatg ataatattag tatcttttaa tgtatttctt gatacatata taaaaacaac
     1261 tgcgaatgaa gaattgacaa agtctacaga aatagtagaa catatggata gtaattttag
     1321 acctattaag ccaccacaag atgatggaaa gcttcctccc aaagtattat ctaattttat
     1381 gagaaatgta caagataaag ttaggatggc agaaacacaa tcagatgctg atgctatggt
     1441 agttgattct aagtataacc tgatatttcc aacaagagag gatgatttct taaaaaatgt
     1501 tgatgagatg gagctgatta gggagtgtat tcaaaatgaa aaattagatt taaattcaga
     1561 tgcaaataca cggatatcta ccggaaataa ggattattat atttcaactg taaaaattac
     1621 tagtatatat ggtgaacaag acaagtatct tatacttttt atagacatat caaaaactct
     1681 aaatctagca caaaagataa atattgtatt aatatctgta atgtgttttg ctggaatatt
     1741 agcaatattt acagctgtga ttttatctga gaaaattgct aaaccgataa aagagttgtg
     1801 tgagtttgca aaaaaaatgg gtcaaggaga tttcaaaaga acctactttg acttttcaga
     1861 taaagaatta gtagagctct caactgttat gaataagagt gctgagtatc tggataagta
     1921 tgataatgaa caaaaaatat tttttcaaaa tgcatctcat gaattaagga caccacttat
     1981 gtctattaaa ggttatgcag aagctataaa atataatgtt atagatagta aacatgcaag
     2041 tgatataatt cttgaagaaa gtgataggtt aagtgatatg gttgaagatt tattatacat
     2101 ttctaaaata gataatataa ctaaagattt tgaacttgta gaatgtgatt taagagaagt
     2161 actttcaaat tgtggagcta ggcaaaatgt aagagctata aataagggga taaaatttat
     2221 ttatgaattt gatgaagaaa tggttttatt tgaatgtgat gaaaaaaaca tttccaaagc
     2281 atttatgaat cttatagaaa atgctttgag atacgctaag agtgaaataa aaatagtgtg
     2341 taagtataat caaaaaaata tagttgtgat aatagaagat gatggaattg gaatcaagaa
     2401 agaagattta cctcatgttt ttgaaagatt ctataaggga gtagggggaa atcatggaat
     2461 tggattatca atagttaaat ctattgtaaa taagcatggt gggcgtatat acgtagaaaa
     2521 tggcaaaaaa ggagctaaat ttactatatt atttaaattg taaaaaaatt agagaaaata
     2581 tagagatgtt tgaagttgtt ttttatatat atctgttgta tactgttgaa aaaagaacaa
     2641 tattaagctg acatatatta cgatagttga catttaaagt aaatgcttaa atatagatac
     2701 gaaaggaaaa gtaaaatctg aagtgtattt aggtttaagt aagcttctta ttttataaaa
     2761 aatatgtatg aaagaacagc tgatttttaa tattacaaat ttagtaacgg ttgtatttga
     2821 agcttttgtc atccatatgt ttttatcaga ctttttaggc cagaaagaag ggtatataaa
     2881 agttgtaaga tatgctaaac tgggttttat aatatgttta ggattctgta atttaataac
     2941 tttaaaccct aaaatcacaa tgcctttaat tttcatcctt attttttcaa catcattttt
     3001 atataaagga aatttaaaga cacgattgtt tactgctgtt ttattaagta tattctttat
     3061 actgtcagaa attgtagtta caagtatttt tgttttattt gtaaaagaag gctttgaaat
     3121 aatgcttgaa aataatagta taagagtatt ggcgacaatt ttatctaaaa ttgtcttttt
     3181 gttgacttgt aaaattattt gtctatttaa aaaagatgta catttagata tgcccataaa
     3241 gtattggctc cctttatttt taatacctat atttagttta tttttgtctg tttctatctt
     3301 tgatgtaagt aaatttttta gtcttgaaag tttaaaattt ctaagcttaa tatctagtgt
     3361 tggtatttta tatattaatt ttattgtctt ttatttattt aaatttatta tagataaaac
     3421 taaactatct atgaaatatg aactacttga aaaagaaata attcataagg aagaattaag
     3481 acttagtaat gagtgttata aaattattgt tgaacagact gattctgtcg ttttcgaatg
     3541 gaatatcaaa gaaaataaat catttgtatc gcaggcttgg actgaaaaat ttggatataa
     3601 caatgcatgt aagaatattt ttaaagaaat taaagataaa gacttagttc attctgaaga
     3661 taaagctata tttgaaggtt ttcttgaaag tattaaaaaa aagaatatgc acaatcaagc
     3721 agtatatcgt ttaaaaaaat ctaatggtga atatatttgg tgtagaacat caattacaag
     3781 tatttataat gatgaaaatg aacttttaag agttgtcgga gtaattgtag atgtagatag
     3841 tgatataaaa aaatatgaag agttaagaac tagagcagaa agtgactcat tgaccaatat
     3901 atacaataaa ggtacatttg aaaaattagt ggaagaaact atagttatga atacaggtga
     3961 taaaaaagat gcattattca ttattgattt ggatgatttt aaagaaataa atgataattt
     4021 tggtcatcca tttggtgatt ttgtactaaa aacatttgct gataagatac aaactagttt
     4081 tggtagtaaa gacttagtcg gaagaattgg tggagatgaa tttgttgtat atatgcaaga
     4141 ttatgttaca gaagtaaatt tgcataaaaa agctaaagaa ttaaatagag tactttcgga
     4201 taattacact gatttgagtt ttagttttga tgcttctgtc agtataggta ttgcaaggta
     4261 tcctcaagat ggaacttcat tttttgaatt gtttaaaaat gcagatagag cactttatag
     4321 cataaaagca tcaggtaaaa attcatattg tttatttgaa gaagaactat atgtacagtg
     4381 aatttgaatt tagattttat gtgagagtaa gatttatata tacttgtggc tgtctgattt
     4441 ataaaatatg ttactttgta tatttttata tgtttcagta aggttaatta acataagcaa
     4501 aggtgtaagt aaatttatat tagtttaagt ttacttacac ctttataaaa atacaaataa
     4561 tagattcatt tacattctta aatgattaaa gctctttacc ttctaaacat gcataacata
     4621 tcgttataat tccagtaaat aagactaaaa tgccattcct aagattacag tttcatcatc
     4681 tattactcca aatgttttta aagtaactta tttcgctttg tatcataata atttatctcc
     4741 ttttacattt taacttctgc tgagatttga gttagatatt cgttagaatc tcgcacactt
     4801 atatcatata gaagatattc ttcataaaca aaatccccaa tacgtaaatt attctgatta
     4861 aaaaaattta ataacttttc ataagtcttg taaatagttt cataactccc tttatgataa
     4921 gcagttacat aaagtccctt tggtctgatt gatacaggat atttcttgta ctctttatta
     4981 acttttgtaa ataaatatga atatgattca aagtctttat taattatatc ttcaccttta
     5041 atgattgagc caatcgtacc ataatcactc aattcatagt tgtccataaa attaatacat
     5101 tcatataaaa aattttcttc ctcatctcct aaaaactgtt ctttatatgt aacaggtgtt
     5161 ttgaccaagt actcttcttc atgttctttt aaagtaatag tattcgcatc tatattacaa
     5221 gcatttttag tgaatgaaat agtagattcc attagagctt gaatattatt tagtttttca
     5281 atttcatttt taatatcaat ggttttcttt tctaaaagct caattagtaa attaggtgtt
     5341 cttttgtcta tataagcttt aatttctttt aaaggcattt ttacttccct aagtgaagaa
     5401 attataccaa aaatgctaag ttgatggtat gagtagtatc tatatccatt ttctttcttt
     5461 aattctggtg agaaaagacc tatatcatca taatggaaaa gtgttttttt attgattcca
     5521 caaatctttg caaattctcc agttgtaaaa taattttttg aaacttgact cataattata
     5581 attcctcctt gactatcctg ttacagtata gtttatcata gtattttgta aaggagtgtg
     5641 attatatgaa aaaattaatt cattttttaa aaccatatag agttttaatt gttgtagttt
     5701 tgatatttac gtttttacaa acacttggaa ctttgtatat accaacactt acagcaaata
     5761 tagtaaacaa tggtgtagta aagggagata tagattatat tgtaaaaaca ggtttaatga
     5821 tgatgatagt agctggtata acagctttat cagctgtgtt agtttgtaaa gtatcaacaa
     5881 atctttcatc tggattttgt agagatatta gagaagcagt atttataaaa tcacaagatt
     5941 tatcaataaa tgattttaat aatataggta cagcatcaat gattactagg agtactagtg
     6001 atattacttt aataggtcag tctgtgttta tgtttattca attagtatta ccagcaccaa
     6061 ttataactgt ttctggtcta ttcttagcat attctataga taaagcaatg acaattatta
     6121 ttgttgtagt aatgttttta ttcatgcttt cagcattttt agttggtaaa aagcttataa
     6181 agttgtttaa aatgatgcaa ataaagatgg acaatatgaa tagggtgtta cgtgaggtag
     6241 taactggagt aagagttata agagctttta ataggagtcg ttttgaaaaa aagagatttg
     6301 atagaactgc aatagattat tctgaaacag caatttcaat aaataaaata tttgcagtac
     6361 ttatgcctat tgtaatgtta ataatgaatt taggtatagt aagtattatt tggtttggtg
     6421 ggatgagggt cagtaatgga aatatggaaa taggtcatat aatggcgctt gttgaatatt
     6481 gtatcttaat tttattctat ttaatcatgg gagtaatggt atttatgtac atacctagag
     6541 caggtgcatg tgcagataga gtaaatcaga tattagatac agaacctgaa attgtagatg
     6601 gaaatggtca taaagatact gtctcagaga gaggacattt agtatttaaa aatgtaacct
     6661 tttcatatgc acaatcagaa gaacctgtgt taaataatat aacatttgaa gcaaaatctg
     6721 gagaagtaac tgcaattata ggaagcactg gctcaggcaa atctactatt gcaaatataa
     6781 taccacgttt ttttgaaatt caatctggag aaatatctat aaatggtcag gatattaaga
     6841 agattcctca aaaagaactt agagataaga ttggatttgt accacaaaaa gcttttttat
     6901 ttagtggaac aatagaagaa aatataaggt atggaaaaga agatgcaagt attgaagaag
     6961 taaagcatgc tgcaagtata gctcaagctg atgaatttat ttctgatatg gaagacaagt
     7021 atgactcatt tgtagcacaa ggtggaaata atttgtcagg tggtcaaaaa cagagaatat
     7081 ccattgcaag agcgttagtt agaaagcctg aagtatatgt ctttgatgat agtttttctg
     7141 cattagattt taaaacagat aaaagactta gaaaagcgtt gaaaaatgaa atcaaagatt
     7201 catcagctat aattatagca caacgtataa gtacaatcat ggatgctaat cagattatag
     7261 ttttaaatga tggaaaaatt gtaggtattg gaaaacataa agatttactt gaaaattgtg
     7321 aagtttataa gcaaattgcg gattcacaac ttagtaaaga agaacttgca taagatgaag
     7381 aattaatctg atatttggag gtgtatgata tgagtgaaaa aagaatagat gaacaagata
     7441 tagaagtact tagttttgaa gatggcatgt cagcagaaaa agctaaggat gcaaaaaaaa
     7501 ctactaagag attattaaaa tatatggcaa aacaaaagtt taaaatatta attattttta
     7561 tttcagtatt gataagttca gcgcttacag ttttagcacc tatggttatg ggaaaagcta
     7621 ttgaccaact atttaatgga ataaaaactg cagtccaaac aggtacaaaa ttcagtgtga
     7681 attttagtac tatggggggt atagtatcaa tacttctagg gctgtactta attagtgcag
     7741 tatttatata tattcaacag tatattatgt caggagttgc acaaaatcta gttcttagca
     7801 tgagaaaaga tttaagtgat aaactcaata aacttcctct taagtactat gattctcata
     7861 aaaaggggga aacacttagt atagtaacaa atgatttaga aaaagtagca gatagtttac
     7921 aagaaggatt aatgcaactt attacagcag tagttacagt agttggttct attgtaatga
     7981 tgataagtat aagtgtacca cttacaatag tatcagctat aacattactt gtttcattag
     8041 gtattactgt tgtaattgca agaaaatcac aagaaagatt ctctgaaaat caaaaggcac
     8101 taggagagtt aaatagcaat atagaagaga tatttactgg tcaaatagtg gttaaagcat
     8161 ttagtaaaga aaaggatact ataagaaact ttaaaatatt aaatcaaaat ctttataatg
     8221 caagtagaaa agcacagttt tcgtcatatg caatatctcc aattattaga tttataaacc
     8281 taattggcta tgttattata gcagttgtag gtgggatatt tgcatcaact ggagcaatga
     8341 ctcttggtag tatacaagcg tttattcaat atgtaaatca agcatcagag cctactacag
     8401 aaatatctta tattgtaaat atgcttcaag cagcaatagc atctgcagag agagttttta
     8461 cagttatgga cgaggtggaa gagataaagg atgaagaatc ttcaaaggtt attagcatgc
     8521 ctaaaggtaa agtacaattt gagcatgtta aatttggata tagtgatgat tttattctta
     8581 tgaaagatat taatatcaat ctaaatgcag gagataagat tgcaattgtt ggacctacag
     8641 gagctggaaa gacaactctt gtaaatttac ttatgagatt ttatgagata caaggtggaa
     8701 ggattactat tgatggtgtt aatataaaag atttaaagcg tggagaactt agaactatgt
     8761 ttggtatggt tcttcaagat acatggctgt ttaatggaag tataaaagaa aatattgcat
     8821 atagtaagtc agatgccaca atggatgaaa ttgtaagagc agcaaaatca gcaagagtag
     8881 accattttat aaggacttta ccacaagggt atgacactat attgactgag gatgcttcta
     8941 atctatctca aggtcaaaag caacttctaa caattgccag ggcaatactt tcagacccat
     9001 cagtattgat tcttgatgaa gcaacatcta gtgtagatac tagaacagag gtagaaattc
     9061 aaaaggcaat gaataacttg atgaagggaa gaactagttt tgttattgca catagattat
     9121 caactattcg agatgctgat ttaatattag taatgaaaga aggaactatt attgaacaag
     9181 gaagccataa ggaacttatt gaaaagaaag gattctatga agaactttat aacagtcaat
     9241 ttacaagtga atatgatgaa gatgttgtat gattatcaag ttttaggcaa ctaaatgatt
     9301 gataaatatt aagttaaata tagtaacatg taaggttata tggaataaga gtatttctct
     9361 atttcatata acctttttta tttatcaaaa taagataaaa tttcctttct aaattagatt
     9421 gaagaatgta tttatcaata taaaagtaaa ataatcatta ttattagtga agtaaatatt
     9481 gtttgccaca tatatgccat aaagatgcac ttattttacg gagatttaac aaagtagtgt
     9541 aaagtatact ctaaatataa aataaatcaa atagagttaa ataaattgag aggtgatata
     9601 aatagtgaat aaaaaattga actatagatt tgaaatgaag cacaaaataa ctgaagcaga
     9661 tgtgttagct ttaaaatcaa gactctatcc aataatgaag aaagatgaaa atgcatctaa
     9721 agatggaaaa tatcttatta ggagtttata ttttgatact cctgaagata aagcactttt
     9781 ggataaatta aatggcgttg caataagaga aaagtttagg ataagatttt ataataatga
     9841 ctgctcttat ataaggttag agaaaaaaat caaacattac aacatgacat caaagctatc
     9901 aactaattta accaaaaaag aagtaataaa tatactaaat aatgatattg aatttctaaa
     9961 agagtcgaca aattgtttat taagagaatt ttatttgaaa ttaaaatgtg aaagattaga
    10021 ggcaaaaagc atagttgatt acaatagaga agcttttatt tatccagttg gaaatgtaag
    10081 agttacaatc gatagtgata ttaaaacttc tgtaaattcg gtagatttat ttaataaaga
    10141 tttgccaact atttcggtaa ttgatgagaa tatgactgta ttggaggtta aatatgatga
    10201 atttatacca gattttataa aggatttaat tcaaattaat aagactacct ctacagcagt
    10261 ttcaaagtat gcttcaagta gattatatat ttaagaataa aggctatgat ttatataaag
    10321 atatacataa ataaggttaa aaaacaaata agttaaaaat aaataaaata aaaaataaat
    10381 aagttaaaaa taggaaggat gattttaatt atgacattta atgatatttt taaatcaagt
    10441 tttatagaaa acgtatcagg tttttcattt gtggacagtg ctttagcact aggttcagct
    10501 tttttagtag gtttgtttat atatatggtc tataaaaaga cttatatggg aattatgtat
    10561 tctcgaccat tcaatgtgtc gcttgtagca cttacaatgc ttacaacttt tgtaatattg
    10621 gcggttactt caaatgtagt tttatcctta ggtatggttg gtgctttatc tatagtaaga
    10681 tttagaacag caattaaaga cccaatggat ttagtatttc tattttggtc attaggttca
    10741 ggtatagttt taggagcagg tttgattccc ttagctgtca tgggttcaat aattatggga
    10801 ttgattctaa tattcttttc aaataaaaca atatctgaaa ctccatatat attgatggta
    10861 aattgtaata atgaagattc agaagatata gcaactgata aaattaaaaa agtatttact
    10921 aaatatcaaa taaaatcaaa aagtgttaca ccagaaaaag gaatagaatt agtgtttgaa
    10981 gtaagaatga aagatggaga aacaagtctt ataaatgact tgagtcaagt agatggagtt
    11041 actaatgcag ttcttgtaag ttataatggt gactatgtag catagagagg agtaatcttt
    11101 tatgaaagat aaaaaattta cccttcttat ctcgattatg attatatttt tatgtgctgt
    11161 agttggagtt tatagtacat ctagcaacaa aagtgctgat ttatatagtg atgtatatat
    11221 tgaaaaatat tttaacagag acaaggttat ggaagttaat atagagatag atgaaagtga
    11281 cttgaaggat atgaatgaaa atgctataaa agaagaattt aaggttgcaa aagtaactgt
    11341 agatggagat acatatggaa acgtaggtat aagaactaaa ggaaattcaa gtcttatatc
    11401 tgtagcaaat agtgatagtg atagatacag ctataagatt aattttgata agtataatac
    11461 tagtcaaagt atggaagggc ttactcaatt aaatcttaat aactgttact ctgacccatc
    11521 ttatatgaga gagtttttaa catatagtat ttgcgaggaa atgggattag cgactccaga
    11581 atttgcatac gctaaagtct ctataaatgg cgaatatcat ggtttgtatt tggcagtaga
    11641 aggattaaaa gagtcttatc ttgaaaataa ttttggtaat gtaactggag acttatataa
    11701 gtcagatgaa ggaagctcgt tgcaatataa aggagatgac ccagaaagtt actcaaactt
    11761 aatcgttgaa agtgataaaa agacagctga ttggtctaaa attacaaaac tattaaaatc
    11821 tttggataca ggtgaagata ttgaaaaata tcttgatgta gattctgtcc ttaaaaatat
    11881 agcaataaat acagctttat taaaccttga tagctatcaa gggagttttg cccataacta
    11941 ttatttatat gagcaagatg gagtattttc tatgttacca tgggatttta atatgtcatt
    12001 tggtggattt agtggttttg gtggaggtag tcaatctata gcaattgatg aacctacgac
    12061 aggtaattta gaagacagac ctctcatatc ctcgttatta aaaaatgaga catacaaaac
    12121 aaaataccat aaatatctgg aagagatagt aacaaaatac ctagattcag actatttaga
    12181 gaatatgaca acaaaattac atgacatgat agcatcatat gtaaaagaag acccaacagc
    12241 attttatact tatgaagaat ttgaaaaaaa tataacatct tcaattgaag attctagtga
    12301 taataaggga tttggtaata aagggtttga caacaataac tctaataaca gtgattctaa
    12361 taataattct aatagtgaaa ataagcgctc tggaaatcaa agtgatgaaa aagaagttaa
    12421 tgctgaatta acatcaagcg tagtcaaagc taatacagat aatgaaacta aaaataaaac
    12481 tacaaatgat agtgaaagta agaataatac agataaagat aaaagtggaa atgataataa
    12541 tcaaaagcta gaaggtccta tgggtaaagg aggtaagtca ataccagggg ttttggaagt
    12601 tgcagaagat atgagtaaaa ctataaaatc tcaattaagt ggagaaactt cttcgacaaa
    12661 gcaaaactct ggtgatgaaa gttcaagtgg aattaaaggt agtgaaaagt ttgatgagga
    12721 tatgagtggt atgccagaac cacctgaggg aatggatggt aaaatgccac caggaatggg
    12781 taatatggat aagggagata tgaatggtaa aaatggcaat atgaatatgg atagaaatca
    12841 agataatcca agagaagctg gaggttttgg caatagagga ggaggctctg tgagtaaaac
    12901 aacaacatac ttcaaattaa ttttaggtgg agcttcaatg ataataatgt cgattatgtt
    12961 agtaggtgta tcaagggtaa agagaagaag atttataaag tcaaaataag attaaaattg
    13021 aatataaata attttaatac tgttaagtct aaatatttac ttagcagtat ttttttagaa
    13081 aataatgtat ttggttaata atataaggta ataaa
//
LOCUS       DBGJFF010000108        12885 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.109, whole genome shotgun sequence.
ACCESSION   DBGJFF010000108 DBGJFF010000000
VERSION     DBGJFF010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12885
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.109"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            169..1440
                     /locus_tag="ACOIAF_002924"
                     /pseudo
     CDS             169..1440
                     /locus_tag="ACOIAF_002924"
                     /inference="COORDINATES: protein motif:HMM:NF012593.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
     gene            complement(1604..2287)
                     /locus_tag="ACOIAF_002925"
     CDS             complement(1604..2287)
                     /locus_tag="ACOIAF_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HGZ2669926.1"
                     /translation="MKKFLEKHIDLLKKIPLFEGIKSDELEEMLECLGVVDKTYPKDS
                     IIFSVGSEVTSIGIMLKGSAHIIKEDIEGNRNIVAELLPGDLFGEVFACTRLHKSNVT
                     VTTTSSCEVLFIKFKSVTGICSSACVFHNRLVENMLQLIAEKNILLHNKIELLSRKTT
                     REKLLMYFSKQIEQTGSHQFTIPFSRNEMADFLCVDRSSMSRELGKMRDEGLLSFNKN
                     KFEIFYFQK"
     gene            2473..3198
                     /locus_tag="ACOIAF_002926"
     CDS             2473..3198
                     /locus_tag="ACOIAF_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGZ2669927.1"
                     /translation="MMRKIIKIDEEKCNGCGLCVEACHEEAIGMVNGKAKLLRDDYCD
                     GLGDCLPTCPTNAISFEYREAAEYDEAAVKANMEAKKAQKKTLACGCPGSQSKSINRE
                     VSNSTSISNDIVEIKGSQLNQWPVQIKLVPTNASYLKNASLLIAADCTAYAYGNFHNK
                     FMKNKVTLIGCPKLDEVDYAEKLTEILKENDIKNIVVTRMEVPCCGGIVNAVKTALQN
                     SGKMIPWQIVTISVDGKIVDGEI"
     gene            3258..4850
                     /gene="hcp"
                     /locus_tag="ACOIAF_002927"
                     /gene_synonym="priS"
     CDS             3258..4850
                     /gene="hcp"
                     /locus_tag="ACOIAF_002927"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HGZ2669928.1"
                     /translation="MEKMFCFQCEQTAGGKGCTINGVCGKKGGTANLQDELTGVLIGL
                     SRATIGNKNRPTSETDKIMIEGLFTTITNVSFDDEAIKRQIEKTEVEKSKLVPRCSDC
                     STTCNRNDNYDMKDLWNDNEDIRSLKSLILFGLRGMAAYAYHAMVLGYTDKDVNEFFY
                     EGLIAVGGDLSIDELLGLVMKTGEINLKCMELLDRANTETYGTPEPTQVSMKIEKGPF
                     IVVTGHDLYDLHKLLEQTKDKGINIYTHGELLPAHAYPKLKEYKHLKGNFGTAWQNQQ
                     KEFDNIPAPILFTTNCLMPVKDSYRDRVFTTEVVAYPGMVHIDEDKDFSSVIEKALEL
                     EGYREDVHMTGINGGDTLTTGFARGTVSSVQDKVVDAVKSGAIRHFLLVGGCDGAKPG
                     RNYYTEFVKQAPKDTVILTLACGKYRFNDLDLGDIGGLPRLMDMGQCNDAYSAIKVAV
                     SLANAFECGVNDLPLSMVLSWYEQKAVCILLTLLHLGIKNIYIGPSLPAFLSKNVLDI
                     LVDKFALTPISTPEEDMKKILG"
     gene            5222..6127
                     /locus_tag="ACOIAF_002928"
     CDS             5222..6127
                     /locus_tag="ACOIAF_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HGZ2669929.1"
                     /translation="MKNSNFNILAFDGGGLKGALSISILERIVKEYPNLLNNINMFGG
                     TSTGSLIALGLAYGVSPKEIKELYSIENSKYIFNKSYAEILRPKYENKNLKEVLLSIF
                     PEELELKDLNKLVMIPSFYIGNEENAWKPVFYNNMPNSFTKTSKVVDVAMASSAAPVF
                     FPTYNRHVDGGIIATDPSLACIIHAIDSGFRLKNTRLFSIGTGYVYNSIKADTTEWGA
                     IDWIINKEPDLPIISITLEGNSQMSQLFSQKLLGDNYYRLNPKMEKDVAMDDCDALEY
                     LMSLGENCDIKDAFGWIKKKSFYRN"
     gene            6251..7204
                     /gene="msrA"
                     /locus_tag="ACOIAF_002929"
     CDS             6251..7204
                     /gene="msrA"
                     /locus_tag="ACOIAF_002929"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HGZ2669930.1"
                     /translation="MIKLATFAGGCFWCMVKPFDKYDGVIKVVSGYTGGHVENPTYQE
                     VCSGTTGHLEAIQITYDDDIISYDELLNIFWKQINPTDDGGQFGDRGTQYKTAIFYHD
                     EEQKNIALESREHLENSKIFDSKIVTDIIPATKFYEAEENHQDYYKKRPLNYNMYYKG
                     SGRYNFVKSHWDGNNINREELKKTLTPIQFEVTQNDMTEVPFENEYYDNNKEGIYVDI
                     VSGEVLFSSKDKFDSGCGWPSFSKPVIDKSIIERSDFSQGMYRVEVRSAKGNSHLGHV
                     FDDGPKELGGLRYCINSASLKFIPRENMEALGYGEYLKLFE"
     gene            7357..8487
                     /locus_tag="ACOIAF_002930"
     CDS             7357..8487
                     /locus_tag="ACOIAF_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HGZ2669931.1"
                     /translation="MNLKDFNGKRLKSARIFRAKTIEQLSKETKINKKDLKAFEENKY
                     VPNIENTLKLSNILNFPKEYFYKNENINIVVEDSHFNPQSKLPRVEEISYREKIIIIH
                     KIYSFMENYIKFPKENLPNRKVMSDININDIESLAYKTREFWDLNSTPIVNMVSLLES
                     KGIIISGMNVDRKGATIFTQKQRISKESKYLISLGNDKKSASIRNYTLACELGYIISS
                     ELRIPSKQFSEDEYACAFLLPKESFLKDLTHPEDLDYYVELKKKWIVPISAMIFRAYN
                     LEKINYKKYNYLMNEMDKKGWLIEEPLDKMKGSSPTYLKRAVELLIENKIMSVNSIVS
                     SLEEFGINLYPEDLELLMGLKKGLLSQEVNKKSKVIKFDGKK"
     gene            complement(8604..9572)
                     /locus_tag="ACOIAF_002931"
     CDS             complement(8604..9572)
                     /locus_tag="ACOIAF_002931"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HGZ2669932.1"
                     /translation="MAIKPRKISIVGSGHVGSHCGFSLITQGVCDELFMIDIDESKSK
                     AQALDLADAVSYLPHKVHIEKGTFSDCKDSDIVVISVADSSEGPLRRQNTTRLDLLRP
                     TIGMIKSIVKPIVDSGFDGIFVVISNPVDVVTNYIWEKSGFPKNKVIGTGTALDSTRL
                     RRILSEETGIAQQSIQAYSMGEHGDSQMVPWSHVSIGGKPILDMIKDNPSTYSNLDLP
                     SIVEKNKKTGISIINGKDCTEFGIGTALVEIVKAILHNEKKVLPVSTLLEGQYNERNV
                     FAGVPCVIGKDGIEQIIEINMTEYEQNEFNKSCSVLREYIALSKTL"
     gene            9907..10095
                     /locus_tag="ACOIAF_002932"
     CDS             9907..10095
                     /locus_tag="ACOIAF_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669933.1"
                     /translation="MNYHTKEELVEALRVVSSSIINCGKGQKKFSEETSHHTCFKNII
                     EVMYISKSLIMDEISKRD"
     gene            10355..11554
                     /locus_tag="ACOIAF_002933"
     CDS             10355..11554
                     /locus_tag="ACOIAF_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HGZ2669934.1"
                     /translation="MEKLYYTNQYIRDFTAEIIEIKEVENKYHVLLDKTAFFPGGGGQ
                     FGDLGKIENDDVINVYEDGKKIYHVLDKKPIKIHKVKCSIDWDRREDGMHQHFGQHVL
                     SGCFFKTFNANTTGFHLGNEYSTVDIEGNLEEDKIREIELFANEIIRQNIQVETLLPT
                     KKELKKIWLRRDLPNTDEEIRVIKIGDLDTNACCGVHPKSTGELRMIKIKKWEKHKSS
                     TRIEFLAGKRAVDEVLKRDIYLTKICRYLSCSEEDAINGIVNLNSKVEEALIKKRRLE
                     EVVSTYQVKEMIEKANKVGNISVIKKIYDDEDLKYVNKIANKIAEYENSIALLAVKFN
                     DKINLVFACSKNLKDINMGVLLKDAINLIDGKGGGSKVLAQGGGKNNGNLEATLDYAF
                     MKIEKTL"
     gene            11606..12109
                     /locus_tag="ACOIAF_002934"
     CDS             11606..12109
                     /locus_tag="ACOIAF_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HGZ2669935.1"
                     /translation="MEQQKDYIELYENEELIEIREARMDDLDTIAKFNYNLAKETEGK
                     ELDMDVLTKGVKALLLDERKGKYHVYTVFDKVVAQIMYTYEWSDWRNGNFLWIQSVYV
                     DKEYRRKGIFNYLFNYIKNICDKDENIVGMRLYVEKENINAKATYESLNMYECDYNMY
                     EYEVIHS"
     gene            12216..12533
                     /locus_tag="ACOIAF_002935"
     CDS             12216..12533
                     /locus_tag="ACOIAF_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669936.1"
                     /translation="MGVLKGMFYVFLFLDLVSIFCFFFNKGKIASNKIVFNAIGVLTF
                     VLCFMLFSYYPNNNLIGKFIASLFFIFGVAGVMLKEKNFLYARLLLTVVIVFSTLRLF
                     VIQ"
     gene            12691..>12885
                     /locus_tag="ACOIAF_002936"
     CDS             12691..>12885
                     /locus_tag="ACOIAF_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HGZ2669937.1"
                     /translation="MIESKKSEKLLLSIIYIIFLVYILMIIRIILFRDVPIYAIFKGT
                     FRNVNLIPFYTIYQFIVDSNL"
BASE COUNT         5003 a         1368 c         2222 g         4292 t
ORIGIN      
        1 acgttaaaaa tacaaaaaaa ctatagaaat tgacatatat tccagtattt gctaaagtag
       61 ctgttgggga aaattacaaa aattattaat gcgcattaga taatttactt taattttttt
      121 agcatttggg gtaatttata atataaagca ttagaaagga aaatataaat gagggacttt
      181 ttaaaaaatt ataagtcatc aattatactt ttagcatgta ctatagtagg ggcgatagta
      241 ggattagtac ttggtccaaa agcttcaata ctaaaaccat ttggagattt attcataaat
      301 gctctattaa tgatagtagt accattagtt tttcttagtg tttcttcagg tgttgctagt
      361 ataaaaaata tggaacgatt tggaaaaata atgaagggta cagctatagt atttatggga
      421 acaacacttg taattgcatt tttaggtatt acatctgcat tattatttaa tccgtctaaa
      481 ggattagatg tatcaagtgt aaaaaattta atggtggctc aagatgcaga aactttagaa
      541 tcaaaaggaa cgttattaga ccaagtagta tcaagtatta cagttaaaaa ttttggagat
      601 ttattttcca gtagtaattt actacaatta gttatatttg cagttttaat tggaacagca
      661 atagctatga tgggagatag gggaaataca ataagaaagc ttttatcaga aggaacggaa
      721 gcaatattaa aagtagtaag tatattcatg ctttatgctc ctatagggct aggctgttat
      781 acagctacaa taattgggga acttggaagt aaaataattc aaggatatgt aagggtgttt
      841 gtaatttatt cactaacagc agccatgtat ttcttattat tttacagtct gtatgctttt
      901 ttatctgcag gaaaacaagg gtttagacaa ttctggagaa atgctataaa accaatagca
      961 acggcactag ctacttcttc aagttcagca tgtataccat caaatttaga agctagtaaa
     1021 aatataggag ttcctgatga tatatcagaa acagttatac ctcttggaat aaatatacat
     1081 aaagatgggg cagttttagc agtaataata aaagtggcat ttttatcagg tattttaggt
     1141 agtggaatgg caaatccagc aacgataggt caattattta taactgtatt tgttgttgga
     1201 gtatttatgg ctcctgtagc aggtggctga cttattggtg agatgctcgt ggttagcatc
     1261 ttcggttttc cagtggaggc aattccactg ttaacagtta taactactat aactgatatt
     1321 cctaatacta tattaaatac aactggaaat acagtatcat caatgttagt ttcaagatta
     1381 gtagaaggaa aagattggct tatagataaa acagcaatta ctacaaagaa aataagctaa
     1441 ttgtacactt aattaataaa gttaataggt atcttaattt taataaatta gatacctatt
     1501 aactttttaa gtaaaaatat aaatttaaga ttcaaatatg atatttataa tttgaatcaa
     1561 ctaatatagt atatattgaa ataaatatgt ttaaatatct attttatttt tgaaaataaa
     1621 agatttcaaa tttatttttg ttaaaagata acaagccttc atctcgcatc ttgccaagct
     1681 ctcttgacat agaacttctg tcaacacata aaaaatcagc catttcattt cttgaaaagg
     1741 gaattgtaaa ctggtggcta cctgtttgtt ctatttgttt tgaaaaatac ataagcaatt
     1801 tctcgcgagt tgttttacga gaaagtaatt ctattttatt gtgaagtaaa atgttttttt
     1861 cagctattaa ttgtagcata ttttcaacaa gcctattatg aaatacacat gcagatgaac
     1921 aaataccagt tacactttta aactttataa ataaaacttc gcaagaagat gttgtggtta
     1981 ctgtaacatt acttttatgt agtctagtac atgcgaatac ctctccaaat aaatctccag
     2041 gcaagagttc agctacaata ttcctattcc cttcgatatc ttcctttatt atatgagcac
     2101 ttcctttaag cataattcca atagaagtaa cttcacttcc aacagagaat attatactat
     2161 cttttgggta agtcttatct acaacaccta aacattccaa catttcttca agttcatcac
     2221 ttttaattcc ttcaaataga ggaatttttt taagtaggtc tatatgcttt tctaaaaatt
     2281 ttttcaaata atcacttcct tgttgcatat gcaaccgata atttgttttc attgtactat
     2341 actaagttta taaaagcaag gggatataaa aatcctacta caagaaattt gaaataaaga
     2401 aatataggta ttaaaaataa atatataaga aatcaatact ttaaattaaa aaaattttat
     2461 gaggtgatat aaatgatgag aaaaataatt aaaattgatg aggaaaagtg taatggttgt
     2521 ggactatgtg ttgaagcttg tcatgaagaa gctataggaa tggtaaatgg aaaagcaaaa
     2581 cttttaagag atgactattg tgatgggctg ggagattgtc tgccaacttg tccaacaaat
     2641 gcgatttcat ttgagtatag agaagcagca gaatatgatg aagctgcagt aaaagcaaat
     2701 atggaagcaa agaaagcaca aaagaaaact ttagcatgtg gatgtccagg ttctcagtct
     2761 aaatcaataa atagagaggt ttcaaatagt acgtctataa gcaatgatat agtagagata
     2821 aaaggttctc aacttaatca atggccagta caaataaaat tagtaccaac taatgcatca
     2881 tatttaaaga atgcaagtct tttaatagca gcagactgta cagcgtatgc atatggaaat
     2941 tttcataata aatttatgaa aaataaggtt acattaatcg gatgccctaa acttgatgaa
     3001 gttgactatg ctgaaaaact tacagagatt ttaaaagaaa atgatattaa aaatatagta
     3061 gtaacacgta tggaagttcc ttgctgtggt ggtatagtaa atgctgttaa gacagctcta
     3121 caaaatagtg gaaaaatgat accatggcaa atagttacta tatctgtaga tggaaaaatt
     3181 gtagatggag aaatttaatt aagtgaatta tagaaaaata taataaattt taataaataa
     3241 aaagggagga ataaaaaatg gaaaaaatgt tttgttttca atgtgaacaa acagcaggag
     3301 gtaaaggttg tactataaat ggtgtctgtg gtaaaaaggg gggtacagct aacttacaag
     3361 acgaacttac aggagtgttg attggtcttt caagagcaac tattggaaat aagaatagac
     3421 caacaagtga aacagataaa attatgatag aggggctatt cactactata acgaatgtaa
     3481 gttttgatga tgaagctata aaaagacaaa ttgaaaaaac tgaggtagaa aaaagtaaac
     3541 ttgtacctag atgttcagat tgttcaacaa cttgtaatag aaatgataac tatgatatga
     3601 aggatttatg gaatgataat gaggacatac gttcattaaa atcattaatc ttatttggat
     3661 taagaggtat ggctgcctat gcatatcatg caatggtact tggttataca gacaaagacg
     3721 taaatgaatt tttctatgaa ggattaatag cagttggagg agacttgtct attgacgaac
     3781 ttttaggtct agttatgaaa actggagaaa taaacttaaa atgtatggaa ttgcttgata
     3841 gagcaaatac tgaaacttat ggaacaccag aaccgactca ggtatctatg aaaatcgaga
     3901 aagggccatt tattgtagtt acaggacatg acctttatga tttacacaaa ttattagaac
     3961 aaacgaaaga taagggtata aatatatata ctcatggtga gttattacca gcacatgcat
     4021 atccaaaact aaaagaatat aaacatctaa aaggtaattt tggtacagca tggcaaaacc
     4081 agcaaaaaga atttgataat attcctgctc caatattatt tacaacaaac tgtttaatgc
     4141 ctgttaaaga tagttataga gatagagtat ttactacaga agtagttgct tatccaggta
     4201 tggttcatat tgatgaagat aaagattttt ctagtgtaat agaaaaagct cttgaacttg
     4261 aaggatatag agaagatgtt catatgactg gtattaatgg aggagataca ttaactactg
     4321 gttttgcaag aggtacagtt tcatctgtac aagataaagt agtagatgct gtaaagagtg
     4381 gagctataag acacttttta ttggttggag gttgtgatgg tgcaaaacct ggaagaaatt
     4441 attacactga atttgtaaaa caagctccta aagacactgt tatattgaca cttgcatgtg
     4501 gaaaatatag atttaatgat ttagaccttg gggatatagg tggcttacct cgtttaatgg
     4561 atatgggaca atgtaatgat gcttatagtg caattaaagt ggcagtttca ttggcaaatg
     4621 cttttgaatg tggagtaaat gatttgcctc ttagcatggt actgtcatgg tatgagcaga
     4681 aagctgtttg tatacttctt acactacttc atttaggtat caaaaatatc tatatagggc
     4741 cttctttgcc agcattctta tctaagaatg tattagatat attggttgat aaatttgcat
     4801 tgacacctat atctactcct gaagaagata tgaagaagat tttaggataa ataaacatat
     4861 ctatacttaa agatatttat agttaaaata tttttaagta taccacaatt aagataaggg
     4921 gttaaactta aaatttgatt aaaaaatcaa ttttgagatg accctttatt tattttttgt
     4981 aataattgaa aaataaacct gctttcagta tattcttttt aagaatcttt gtattaaatt
     5041 tataatactt agatatataa tatcttatat attttatttt aagtattaaa ggtttgaatg
     5101 taacttaaat attaaaagag gaacattgta atatgtaact tgcataaaat atatatacta
     5161 tagtgaaatt attttgatta aaatttaaaa tataaggtgt tttatgtggg ggtatatgta
     5221 tatgaaaaat tctaatttta atatattggc atttgatggt ggtggattaa aaggggcatt
     5281 gagtatatcc atacttgaga ggatagtaaa agagtatcca aatctattaa acaatataaa
     5341 tatgtttgga ggtacatcta cagggagtct aattgcacta gggcttgcgt atggtgtaag
     5401 ccctaaagag ataaaagaac tttactcaat tgaaaattca aagtatattt ttaataaaag
     5461 ttatgcagaa atactcagac caaagtatga aaacaaaaac ttaaaagaag ttttattaag
     5521 tatatttcca gaggaactag aattaaaaga tttaaataaa ttagtaatga taccaagttt
     5581 ttatatagga aatgaggaaa atgcatggaa accagtattt tacaacaata tgccaaattc
     5641 ttttacaaag actagcaaag ttgtagatgt agcaatggca agtagtgctg ctccggtatt
     5701 ttttccaaca tataatcgtc atgttgatgg aggaataata gcaactgacc cgagtttagc
     5761 gtgtataatt catgccattg acagtggatt tagacttaag aatacaagac ttttttcaat
     5821 agggactgga tatgtatata acagtataaa ggcagatact accgaatggg gagctattga
     5881 ctggataatt aataaagaac ccgatttacc aattatatct attaccttag aaggaaattc
     5941 tcaaatgagt cagttatttt ctcaaaaact tcttggagat aattactata gattaaatcc
     6001 taagatggaa aaagatgtag ctatggatga ttgtgatgca ttagaatatc taatgtcttt
     6061 aggagaaaat tgtgatataa aagatgcttt tggatggata aagaaaaaga gcttttatag
     6121 aaattagatt caatttgcaa ttgtattttt acacatgatt tcattgagta ggtaaaaata
     6181 ataaaatgca gattattaag ttacttctta gggaataaat aaagtataat ttatttaagg
     6241 agtgatgaaa atgataaaac ttgctacttt tgcaggtgga tgtttttggt gtatggtaaa
     6301 accttttgat aagtatgatg gagttattaa agtagtttct ggatatacag gtggtcatgt
     6361 tgaaaaccct acatatcaag aggtatgttc aggaactact ggtcatttag aagcaattca
     6421 aataacatat gatgatgata taataagtta tgatgaactt ctaaatatct tctggaaaca
     6481 gattaatcct acagatgatg gtggacaatt tggtgatagg ggaactcaat ataagactgc
     6541 tatattttat catgatgaag aacaaaaaaa tatagcttta gagtcaagag aacatctgga
     6601 aaatagtaaa atatttgata gtaaaatagt gactgatata attccagcaa ctaaattcta
     6661 cgaagcagaa gaaaatcatc aggattatta taagaaaaga ccattaaact acaatatgta
     6721 ttataaaggg tccggaagat ataattttgt aaaaagtcat tgggatggaa acaatataaa
     6781 tagggaagag ttaaaaaaga ctttaactcc tatacaattt gaagttactc aaaatgatat
     6841 gacagaagta ccttttgaaa atgaatatta tgacaataac aaagagggaa tttatgttga
     6901 tattgtaagt ggagaagtgt tattttcttc aaaagataaa tttgattctg gatgtgggtg
     6961 gccaagtttt tcaaagccag ttatagataa atctatcatt gagagaagtg atttctcaca
     7021 aggtatgtac agagttgaag taagaagtgc taaaggaaat tctcatttag gtcatgtatt
     7081 tgatgatggt ccaaaagaac ttggaggttt aagatattgt ataaattctg cttctcttaa
     7141 atttatacca agagaaaata tggaagcttt aggatatgga gaatatttaa aactttttga
     7201 gtaattagga atatttatta taaacttatc taaaaggtga aaatctaaaa aaatattttc
     7261 accttttgtt tttttgtcaa tagtatgata aattattaat tgtaatctta tttataccaa
     7321 tagattaaga atttaatttt gaaagagaga aaacaaatga atttaaaaga ctttaatgga
     7381 aagagattaa aatcagctag aatttttaga gcaaagacga tagaacagtt gtctaaagaa
     7441 actaaaataa ataaaaaaga tttaaaagct tttgaggaaa ataagtatgt accaaatata
     7501 gaaaatacat taaaattatc taatatatta aattttccaa aagagtattt ttataaaaat
     7561 gaaaatataa atatcgttgt agaggatagc cactttaacc ctcaatctaa actacctaga
     7621 gtagaagaga tttcttatag agaaaaaata attataatac ataaaatata ttcatttatg
     7681 gagaattata ttaagtttcc aaaagaaaac ttgccaaata gaaaagtaat gtcagatatt
     7741 aatataaatg atatagaatc tcttgcatat aagactagag agttttggga tttaaatagt
     7801 actccaatag taaatatggt aagtttgtta gagtctaaag ggattataat atcaggtatg
     7861 aatgtagata gaaaaggggc aactatcttt actcaaaaac agagaattag taaagaatct
     7921 aaatatctaa tatctctggg taatgacaaa aagtcagcat caattagaaa ttatactttg
     7981 gcatgtgagc ttggatatat aatatcaagt gaattaagaa taccaagtaa gcaattttca
     8041 gaagatgagt atgcctgtgc atttttattg ccaaaagaga gttttttaaa agatttgact
     8101 catccagaag atttagatta ttatgtagaa ctaaagaaaa aatggattgt acctatatct
     8161 gctatgattt ttagagcata taatctagaa aagataaatt acaaaaaata caattatctt
     8221 atgaatgaaa tggataaaaa aggctggttg atagaagaac cactagacaa aatgaaaggt
     8281 tcaagcccaa cgtatttaaa aagagcagtt gaattattga tagaaaataa aataatgagt
     8341 gtaaatagta tagtatcaag tttagaggaa tttggaataa atctttatcc agaagactta
     8401 gaattattga tgggattgaa aaaaggattg ttatctcaag aggtaaataa aaaaagtaaa
     8461 gttataaaat ttgatggtaa aaaataggtt tatattttag tattaaaatt aaaagcaggt
     8521 acaaatgatt taaacattaa cttagttaaa agttttttca ataagtcatt tacctgcttt
     8581 tagtgtaaga aagaatattg aatctaaagt gtttttgaaa gtgcaatata ttcccttaaa
     8641 acagaacaag atttattaaa ttcgttttgc tcatattcag tcatattgat ttcaataatt
     8701 tgttcaatac catcttttcc tataacacat ggaacacctg caaatacatt tctttcgtta
     8761 tattgacctt ctagtaatgt agaaactgga agaacttttt tctcattgtg tagtatagct
     8821 tttacaattt ctactaaagc agtacctata ccaaattctg tacaatcttt accatttatt
     8881 atacttattc ctgttttttt atttttttca actattgatg gtaagtctaa attgctataa
     8941 gtacttgggt tgtcttttat catgtctaag attggtttac caccaattga aacatgagac
     9001 caaggaacca tttgagaatc tccatgttca cccatagaat aagcttgaat tgattgttga
     9061 gcgattccag tttcttcaga taaaatcctt ctaagtcttg ttgaatctaa agctgttcca
     9121 gttccaatga ctttgttttt aggaaagcca gatttttccc aaatataatt tgtaactaca
     9181 tcaacaggat tagatatgac tacaaatata ccatcaaagc cagaatcaac tatgggtttt
     9241 actatagatt ttatcattcc aatagttggt cttagtaaat ctaatctagt tgtgttttgt
     9301 ctacgtaaag gcccttctga agagtctgca acacttatca ctactatgtc agaatcttta
     9361 cagtcagaaa aagttccttt ttctatatgt actttatgtg gcagatatga aactgcatca
     9421 gctaaatcta aagcttgagc ttttgattta gattcatcta tatcaatcat aaagagttca
     9481 tcacaaactc cttgagttat taaactaaat ccacaatgag aaccaacatg ccctgaacct
     9541 acaatagata tttttcttgg ctttattgcc attttaaatc actcccccta aaataacgat
     9601 acattgaaaa ttatagatgt tattaaaagc tgtttttgaa tattttaata ttctattatt
     9661 tcagaaaatt tatttaatat gattttatac taaaattttt aaaaaagcaa tgttaatata
     9721 ctctctatta gtgaatatta aagtattata tgtatattaa taattattaa aatatgtaat
     9781 gtcactgata gttagaatat ttaagtgcta aaagatgtgt ataaataata atgatgtcag
     9841 cttataatga tattaaattg agatatactg tttctctaaa ttaaaaaata aaggagtaaa
     9901 tgtgttatga attatcatac aaaagaagaa ttggtggaag cactgagagt agtatcttca
     9961 agtattatta attgtggaaa aggacagaag aaattttctg aggagacatc tcatcataca
    10021 tgttttaaga atattataga agtaatgtat atttcaaaat cattaataat ggatgaaatt
    10081 agtaaaagag attgaagctt attttgcaat ttaacatgtt ttaatataca ttttattata
    10141 taagaaaata agtaaacata catgttataa aatattggaa taatatacaa ttataaattt
    10201 gttaacacta atatctatat gttaaaatat agtagaagat gttagtaaat aataggatga
    10261 gttctatttt aatacaatta aattaagctt agtttgaaat ttgtatctaa aaagtaaagt
    10321 aatctagaat taagaaaatg gagatagaat tataatggaa aaattatact atacaaatca
    10381 gtatataaga gattttacag cagaaataat tgaaataaaa gaagtagaaa ataagtatca
    10441 tgttttatta gataagacag ctttttttcc tggtggagga ggtcagtttg gagacctggg
    10501 aaaaatagag aatgatgatg taataaatgt gtatgaagat ggaaaaaaaa tataccatgt
    10561 attagataaa aaacctataa agatacataa ggtaaagtgt agtattgatt gggatagaag
    10621 agaagatgga atgcatcaac attttggaca acatgtattg tctggatgtt tttttaaaac
    10681 ttttaatgca aatacgacag gttttcattt gggaaatgag tatagtacag tagatataga
    10741 aggtaattta gaagaggata aaattagaga gatagagttg tttgccaatg aaattattcg
    10801 tcaaaatata caagtagaga cattacttcc aacgaaaaaa gaactaaaaa agatttggtt
    10861 aagacgtgat ttgccaaata ctgatgaaga gataagagta ataaaaatag gtgatttaga
    10921 tacgaatgca tgttgtggag tgcatccaaa atcaactggt gaattgagaa tgataaaaat
    10981 aaagaaatgg gaaaaacata agtcaagtac aagaatagag tttctagcag gtaaaagggc
    11041 agtggatgaa gtattgaaga gggatatata tttgactaaa atatgtagat atttaagctg
    11101 tagtgaggaa gatgctataa atggtattgt aaatctaaat agtaaagtgg aagaggcttt
    11161 aattaaaaaa agaaggcttg aagaagtagt atctacatat caggtcaaag aaatgattga
    11221 aaaagcaaat aaggttggca atatttcggt cattaaaaaa atttatgatg atgaagattt
    11281 gaaatatgta aataagatag caaataaaat tgcagaatat gaaaatagta ttgcattatt
    11341 agctgttaaa tttaatgata aaataaattt agtttttgca tgttctaaaa atttaaaaga
    11401 tattaatatg ggagttttat taaaggatgc aataaatctt atagatggca agggtggtgg
    11461 aagtaaagta ttagctcaag gaggaggtaa gaataatgga aatttagagg ctacacttga
    11521 ttatgcattt atgaagatag aaaaaactct ataaatatat tatataaaaa accttagtag
    11581 ataaaataga tatggaggaa taagtatgga gcaacaaaag gactacatag agttatatga
    11641 aaatgaagaa ctaatagaga taagagaagc acgtatggat gatttggata caatagcaaa
    11701 atttaattat aatttggcta aagaaactga aggaaaagaa cttgatatgg atgttttaac
    11761 taaaggtgta aaagcattgt tattagatga gagaaaagga aaatatcatg tatatacagt
    11821 atttgataaa gttgttgcac agattatgta tacatatgag tggagtgatt ggagaaatgg
    11881 aaatttcctt tggattcaga gtgtatatgt agataaggaa tatagaagaa aaggtatctt
    11941 taattattta tttaattata taaaaaatat atgtgataaa gatgaaaata tagttggaat
    12001 gagattatat gtagaaaaag aaaacataaa tgcaaaagca acatatgagt ctttaaatat
    12061 gtatgagtgt gattataata tgtatgaata cgaagtaata cattcgtaat ataaatttgt
    12121 aatactgtta tactgatgtt ataaagtatt gattatattg acgattatat gaaataaact
    12181 ggatattata attagaattt ttagggggta taaaaatggg cgttttaaaa ggaatgtttt
    12241 atgtattttt atttttagac ttagtgtcaa tattctgttt ctttttcaat aaaggaaaaa
    12301 tagcatctaa taaaattgtg tttaatgcaa taggagtact tacatttgta ctttgtttta
    12361 tgctttttag ttattatcct aataataatc ttattggaaa atttattgct tctctatttt
    12421 ttatatttgg agttgcagga gttatgttga aagaaaaaaa ttttttatat gctagacttc
    12481 ttttaactgt agttatagtt ttttctacat taagattgtt cgttattcaa taaaatgtat
    12541 ttgtatgaaa atttattaaa gtctacacat ttaagtgtag gcttttttga ttttttgttt
    12601 gaaaattaag gaatttttaa ggaaatttta aaatcgtctt aaatatttat tgatatactt
    12661 aaaatattaa tagcgaaagg agacttatca gtgatagaaa gtaaaaagtc agaaaaattg
    12721 ttactgtcaa ttatatacat aatatttttg gtatatattt taatgattat aagaataatt
    12781 ttatttagag atgtaccaat ttacgctatt ttcaagggaa catttaggaa tgtaaattta
    12841 attccatttt atacaatata tcaatttata gtagatagta atctt
//
LOCUS       DBGJFF010000109        12878 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.110, whole genome shotgun sequence.
ACCESSION   DBGJFF010000109 DBGJFF010000000
VERSION     DBGJFF010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12878
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.110"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            <1..544
                     /locus_tag="ACOIAF_002937"
     CDS             <1..544
                     /locus_tag="ACOIAF_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HGZ2669938.1"
                     /translation="DLDFNINYNIIEDDSKNTDKLELIFSPNIDKIDLKRYNNIILYD
                     YLYNKGEYSYIYENKREESEIIKYYNKTDLLYLKNVVSNIVPSRDEFITIYKQALIKK
                     EIDLDMVNIRETFNVIPLKFFTILNVFRELNLLDFNLNYEKNSVLIRILPKPQKKLDL
                     NESLILNNLKNLEKQYNSSY"
     gene            557..1069
                     /locus_tag="ACOIAF_002938"
     CDS             557..1069
                     /locus_tag="ACOIAF_002938"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HGZ2669939.1"
                     /translation="MDLKNFIRNIDDFPKPGIDFKDVTTLFKDGDAFKYAVDSIVEEL
                     KDKDVDLVIGPEARGFLMGTPVAYALGVGFVPIRKPGKLPGEVESYEYGLEYGTDTLE
                     IHKDAIKKGQKVAIVDDLLATGGTMEAAAKLVEKLGGEVVSMQFLIELKFLNGREKLS
                     NYDVNSLIKY"
     gene            1143..3350
                     /locus_tag="ACOIAF_002939"
     CDS             1143..3350
                     /locus_tag="ACOIAF_002939"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HGZ2669940.1"
                     /translation="MHDKELQEIIEKIKVYAPNANFEMVEKAYNLAKKAHEGQYRKSG
                     EPYFIHPLAVANILIEMELDIETITAGLLHDVVEDTEYTYEDIKKEFGKEVADLVDGV
                     TKLGQIKYRSKEETQSENLRKMFLAMAKDIRVILIKLADRLHNMRTLKYMPPEKAKYK
                     AKETLEIYGGIAHRLGISKIKWELEDRALRFMDPEGYYDLVSRVSMKRSQREDYIQGI
                     VELLKDKFKDLSIPCDVYGRPKHFYSIYRKMQKKHKTFEEIFDLTAVRILVDNVKDCY
                     AVLGIVHTLWRPIPGRFKDYIAMPKPNMYQSLHTTVVGPDGEPLEIQIRTHEMHNIAE
                     NGIAAHWKYKEGISSKESKVEEKLQWLRQMMEWEKDLKDPQEFMDALKEDVFNSQVYV
                     FTPKGDVIELPAESTPIDFAYRVHTNVGNKCVGAKIDGRIVPIDYKLQNGNIVEVLTS
                     SNSNGPSRDWLNIVKTPNAKSRIRQFFKKERREENVDRGNTILEREFKKHDIPQKDPL
                     VEKYMALVAKKFNQPSVEDLIATVGYGGIMSSQVVSKVKDLYLKEVKKAKKEQQIQEE
                     DLNKHNISETEYSKKRKKNASQGIIVKGLDNILVRFAKCCNPLPGDEIVGYITKGRGV
                     AIHRADCPNANNNGDFFQNRLVEVSWSSAGKGKFEAEIQIKAIDRRGIVNEITHIVAM
                     DKLSLNGINARKGKDSIVSINLLVEVNDTEELKILMKKLKAIPGVESIYRMTN"
     gene            3375..3824
                     /gene="dtd"
                     /locus_tag="ACOIAF_002940"
     CDS             3375..3824
                     /gene="dtd"
                     /locus_tag="ACOIAF_002940"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HGZ2669941.1"
                     /translation="MRAVVQRVSSSKVTVDENTIGQINKGLLVLLGVTHDDKSSDVDY
                     MIDKILNLRIFEDENDKMNLSLMDIGGELLVVSQFTLYGDCRKGRRPGFSNAARPELA
                     NNLYEEFVKKAKDKGVTVGTGQFAAHMMVELTNDGPVTILLDSSKSF"
     gene            3839..4453
                     /locus_tag="ACOIAF_002941"
     CDS             3839..4453
                     /locus_tag="ACOIAF_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HGZ2669942.1"
                     /translation="MIIKKFVDSYFGVNTYVLGDEKTKKCAVIDPGGSLVETLSFVKE
                     NELNIEYIILTHGHGDHIGYVKDIKEKTGAKVVAHVDEKELLNDKNKNLSYTMRCGAQ
                     EFDADIYVNDKDKLELGELKMTFLHTPGHTQGCMCIRVEDEMFTGDTLFAGSIGRTDL
                     YSGDFNQMEKSLKKLCKYEDAVRVYPGHGPTSTLGVEKKTNPYM"
     gene            4544..6100
                     /locus_tag="ACOIAF_002942"
     CDS             4544..6100
                     /locus_tag="ACOIAF_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HGZ2669943.1"
                     /translation="MLYVFLKGHDYKYEVAELIKLFTSEFKFKDSNANNNIESKHLIN
                     RLIYENGVLFSSTEYYENGNLKYESIQNIENMNLEFLDGFDSSKTIEEFLNSLDSESK
                     RNFKKLCKENIKKSMFLVLKQVFNSYVPWGILTGIRPVKIVHNLMDKKLDDNSIRTIL
                     KDNYFIIDEKIDLALEIAKRERLFMYPIDKNKISLYVSIPFCPTRCVYCSFPSNSLKQ
                     FGHLKREYVYKLIEEIKGFAKVIKDTKKEVETLYIGGGTPTTLDEEELDLLINSLFNE
                     LDLSKIKEFTVEAGRPDTITEQKLRVLKKHNVSRISINPQTMNDDTLVKIGREHRVSD
                     LVDCFNMARRMGFDNINMDIILGLIDEDLNMVKNTLEEIKKLSPESLTVHTLAIKRAS
                     NLNINMDRYKEHLTQYEEMVKMIDLSMKYAKDMGLNPYYMYRQKHMLGNLENIGYAKE
                     GYECIYNIQIMEEKQSNYALGAGAISKFVYVDEDRIERVENVKNVEQYIERVDEMIKR
                     KKEEVYKNVD"
     gene            6090..7352
                     /gene="hisS"
                     /locus_tag="ACOIAF_002943"
     CDS             6090..7352
                     /gene="hisS"
                     /locus_tag="ACOIAF_002943"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HGZ2669944.1"
                     /translation="MLTKAPRGTKDITPKEAYKWRYVENKFREICALYGYEEMVTPIF
                     EHTELFKRSVGDTTDIVQKEMYSFKDKGDREITLKPEGTAGVVRAFIENKLYADTQPT
                     KLFYVTPCFRYERPQAGRQRQFHQFGIEALGSDTPSMDAEIIALAVQFFNEVGLNDLV
                     VSINSVGCPVCRKEYNALLKEYLDSKADILCDTCNERREKNPMRVIDCKNPTCKENIK
                     DIPFIADHLCDDCKSHFDKLQEYLKEMNINFVIDKTIVRGLDYYRKTAFEIISNDIGA
                     QSTVCGGGRYDGLVEQLGGPKGISGIGFGLGIERLLLTLEGNGIEIENPQSTDIFIVT
                     IGEEANTRSFKLLKDLRQNHISADKDHIERSVKAQFKYSDKINSKFTIVIGDDELKND
                     TATLKNMKTSEQTTVKLSTLVEELKQKL"
     gene            7426..9213
                     /gene="aspS"
                     /locus_tag="ACOIAF_002944"
     CDS             7426..9213
                     /gene="aspS"
                     /locus_tag="ACOIAF_002944"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HGZ2669945.1"
                     /translation="METLKGLKRTHYCGELREKNINEEVVLMGWVQKKRNLGGLVFVD
                     LRDTSGLCQIVFDTDVDKEAFEKAEKLGAEFVIAVKGKVCERQSKNPNMPTGDIEIFA
                     TELRLLNKSETPPIYIKDDDDVSEALRLKYRYLDLRKPSMQRNLKLRHKVMNITRNYL
                     SNNRFCEIETPFLIAPTPEGARDYLVPSRVNPGKFYALPQSPQLYKQLLMVSGMDRYF
                     QIVKCFRDEDLRADRQPEFTQIDCEMSFVEQEDVMSMIEGLLEAIFKEVLDVELALPL
                     PKMTYAEAMSKYGSDKPDTRFGYELTDISDVVCNCGFKVFADATQPGKSVRGINVKGK
                     ADDFTRKQISSLEEHAKTYRAKGLAWMKVGQEGVTSPIAKFFNEEEMNAILTRMNAEV
                     GDLLLFVADKNSIVFDALGQVRLEVANRLNLLDKNVYNLLWVTEFPVFEEDEETGTFS
                     AMHHPFTSPMDEDLDKLEEGDKSSLRAKAYDIVLNGYEIGGGSVRISNSDVQSRMFKA
                     LGFTEERANEKFGYLLEAFKYGTPPHAGLAFGLDRLVMLLAGTDNIREVIAFPKNQNA
                     VCPMTNAPTLAEDEQLEELSIKVDIKDNE"
     gene            9672..10904
                     /locus_tag="ACOIAF_002945"
     CDS             9672..10904
                     /locus_tag="ACOIAF_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HGZ2669946.1"
                     /translation="MQEREELVLNPVPKEERVGWLAPLFNMLGSNIAISELMVGGTLI
                     LGMTLSNMIITSIIGNLILVAIIMIQGYIGYKEGLNTYVLAKGAFGEIGGKYLISLLL
                     GITSFGWFGVQAGVAGLSVQKIFPSVNLTLVTVILGLFMVVFALYGFKAMAKFNYLVI
                     PPLMILMVWGVFKAFSTYGVEAIYNYTPQTTMSMVEGLNIVVGLVIVGAIISPDQLRY
                     TRRVKDIWIISFLGLGIISLFQQVAAGVMSMGAPTWDITEVLANLGFGWVAFVILILA
                     SWSTNVSNAYSGGLALKTIFPNVKRNVLTLVAGLIGTIIAATGIIFKFQSFLSFLGIA
                     VPAIAGIMWCEYYFIQGRTYKHREGINWIAVISWLIGFAASYYSSKINFLIPPINGIV
                     VSMVIYYILMNCFGIKDK"
     gene            10918..11724
                     /locus_tag="ACOIAF_002946"
     CDS             10918..11724
                     /locus_tag="ACOIAF_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HGZ2669947.1"
                     /translation="MGRKVKIGIIQQHSVLGNVKKNIEKAVEMIDDLGKQGADIICLP
                     ELFATGYNLESLGGVKTLELIREHNKYIEESMSEAAKRNNVYLISPYGTLEKGSTHVY
                     NSAVIFDRKGKIMGEYCKNHLWSLEAVYFKGGEKVEVYDADFGRFGVMICYDAGFPEV
                     SRELTLKGSEIIFIPSAWRIQDEDMWDLNVSQRALENTVYTVGVNLVSNDSNLILFGK
                     SKICNPRGTVITQLDTYREKYVLSEIDLDEIKKYREDIPYLKDRKSSYNI"
     gene            complement(11836..12705)
                     /locus_tag="ACOIAF_002947"
     CDS             complement(11836..12705)
                     /locus_tag="ACOIAF_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGZ2669948.1"
                     /translation="MDLLHLKYFQTVARMEHITKASHKLNIAQPALSKTISSLEKELG
                     VQLFDRKGRYIVLNEYGRLFLKRVDSILDLVESSKKELQDTSLENSGEVKILSPAAAN
                     VLPSLLSNFRKLYPNITFNVSHTLPSSYKKSDFDLYISSSFTKLNSENSITLTCEEIL
                     LGVSINHPLSLKDEVYLSEVSDENFIVITKGENYREVIDILCESANFKPKIAFESDSP
                     YTIYALIKSLQGVGFICGKSWGLSQDPEIKLLHIKDIEFKRYLNLSWFSENYESKAVL
                     LFKNFLINYFKNI"
BASE COUNT         5070 a         1396 c         2382 g         4030 t
ORIGIN      
        1 agatttggat tttaacataa attacaatat tattgaagat gatagcaaaa atactgataa
       61 attagagctt atattttctc ctaatattga taaaatagac ttaaaaagat ataataatat
      121 tattctttat gattatttat acaataaagg cgaatattcg tatatatatg agaataaaag
      181 agaagaaagt gaaattataa aatattataa taaaacagac ctattatatt taaagaatgt
      241 agttagtaat atagttccat caagagatga atttataaca atttataaac aagcactcat
      301 aaaaaaagaa attgatttgg atatggtaaa tataagggaa acttttaatg taattccatt
      361 aaagtttttt acgatattaa atgtatttag agaattaaat ttacttgact ttaatttaaa
      421 ttatgaaaaa aattctgtac tcataagaat attgccaaaa ccacagaaaa aattagactt
      481 aaatgagagt ttaatactca ataatctaaa aaatttagaa aaacaataca atagcagtta
      541 ttaggaggcg tttcccatgg atttaaaaaa ctttataagg aatattgatg atttcccaaa
      601 gccaggcata gattttaaag atgtgacaac tctatttaaa gatggagatg catttaaata
      661 tgcagtggat tctatagttg aagaattgaa agataaagat gtagatttag taataggacc
      721 agaagcaaga ggatttttga tgggtacacc tgtagcatat gctttaggag taggatttgt
      781 tccaattaga aaacctggaa agttaccagg tgaagttgag agttatgagt atggattaga
      841 gtatggtaca gatactttag agatacataa ggatgcaatt aaaaagggtc aaaaagtagc
      901 aatagtagat gatttattgg ctactggtgg aactatggaa gccgctgcca agcttgtaga
      961 aaaattaggt ggagaagtag tttctatgca atttttgatt gagctaaaat tcttaaatgg
     1021 gagagaaaag ttgtctaact atgatgttaa ttctcttata aaatactagt acttttataa
     1081 tagttggtat aacaccaact attttcatat atattaaaat tttatacaaa ataggtggtt
     1141 atatgcatga taaagaatta caagaaataa ttgaaaaaat aaaagtttat gcacctaatg
     1201 caaattttga aatggttgaa aaagcctata atcttgcaaa aaaagcgcat gaaggacaat
     1261 atagaaaatc tggggaacca tactttattc atccattagc agttgcaaat attttaatag
     1321 agatggaatt agatattgaa actataacag caggtttact tcatgatgtt gtagaagata
     1381 cagaatatac ctatgaagac ataaaaaaag aatttggcaa agaagtagcg gatttggttg
     1441 atggagtaac taaacttggt caaataaaat atcgttcgaa agaagaaact caatctgaaa
     1501 atttaagaaa aatgtttttg gcaatggcga aggatataag ggttatactt ataaaacttg
     1561 ctgatagatt gcacaacatg agaacattaa aatacatgcc tccagaaaaa gcaaagtata
     1621 aagcaaaaga gacacttgaa atttatggtg gtatagctca tagattagga atttccaaga
     1681 taaagtggga actagaagac agagcattaa gatttatgga cccagaaggg tattatgatt
     1741 tagttagtag agtatcaatg aagagaagtc aaagagagga ttatattcaa ggaattgttg
     1801 aacttcttaa agataagttt aaagatttaa gtataccatg tgatgtatat ggtagaccaa
     1861 agcattttta cagtatatat agaaagatgc aaaaaaaaca taagactttt gaagagattt
     1921 ttgatttaac agcagtaagg atattggttg ataatgtaaa ggattgctat gcagtacttg
     1981 gtatagttca tacattatgg agacctattc caggaagatt taaagattat atagcaatgc
     2041 caaaacccaa tatgtatcaa tcattacaca ccactgttgt aggtcctgat ggagaacctt
     2101 tagagataca aataagaact catgagatgc ataatatagc tgaaaatggt atagcagccc
     2161 attggaaata taaagaaggt atctctagca aagaatcaaa ggttgaagaa aaacttcaat
     2221 ggttgagaca gatgatggag tgggaaaaag atttaaaaga cccacaagag tttatggatg
     2281 cacttaaaga agatgtattt aatagtcaag tttatgtatt tacgccaaaa ggagacgtaa
     2341 ttgaattacc agcagaatca acacctatag attttgccta tagggttcat acaaatgtag
     2401 gtaataaatg tgtaggagct aaaattgatg gaagaatagt tcctattgat tataagcttc
     2461 aaaatggtaa tattgtcgag gtacttacat catctaattc taatggacca agtagggatt
     2521 ggcttaatat agtgaagacc ccaaatgcaa aaagtagaat tagacaattt ttcaagaagg
     2581 aaagaagaga agaaaatgtt gaccgtggaa atacaatatt agaaagagaa tttaagaagc
     2641 atgatatacc acaaaaagac cctcttgttg aaaaatatat ggcattagtt gctaagaaat
     2701 ttaatcagcc aagtgttgag gacttaattg ccactgtagg ttatggtggt ataatgtcat
     2761 ctcaagtggt atctaaggta aaagacctat atttaaaaga ggttaaaaaa gctaaaaagg
     2821 aacaacagat acaagaagaa gatttaaata aacacaatat aagtgaaaca gaatacagta
     2881 aaaagagaaa gaaaaacgca tcacaaggaa ttatagtaaa agggcttgac aatatcttag
     2941 taagatttgc taagtgttgt aatcctcttc caggagatga aattgtagga tatattacta
     3001 agggtagggg agtagccata cacagagctg attgcccaaa tgcaaacaat aatggtgatt
     3061 tcttccaaaa tagattggtg gaggtttcat ggagttcagc aggaaaaggt aagtttgaag
     3121 cggaaattca aataaaagct attgacagaa gaggaattgt taatgaaata actcatattg
     3181 ttgcaatgga taaacttagt ttaaacggaa taaatgcaag aaaaggtaag gatagtattg
     3241 taagtataaa cttacttgta gaggttaatg acacagagga attaaaaatt ctaatgaaaa
     3301 aattaaaagc aattcctggt gtagaaagta tttatagaat gacaaattag agttaaaata
     3361 atagggagga aactatgaga gcagtagtgc aaagagtatc ttcatctaaa gttacagtag
     3421 atgaaaacac tataggtcaa ataaataagg ggttactagt attgttagga gtgactcatg
     3481 atgataaatc aagtgatgta gattatatga ttgataaaat attgaatctg agaatttttg
     3541 aagatgaaaa tgacaagatg aatttatctc taatggatat aggtggagag cttttagtag
     3601 tatctcaatt tactttatat ggagattgca gaaaaggaag aagacctgga tttagtaatg
     3661 cagctagacc agaacttgca aataaccttt atgaagaatt tgtaaaaaaa gcaaaagata
     3721 aaggtgtgac agttggtaca ggtcaatttg cagctcatat gatggtagag ttaactaatg
     3781 atggtccagt aacaatacta ttagactcaa gtaaatcttt ttaaggggga aaactttaat
     3841 gattataaaa aaatttgtag atagttattt tggagtaaat acttatgtat taggtgatga
     3901 aaagactaaa aaatgtgcag ttatagaccc aggtggaagt ttagtagaga cattaagttt
     3961 tgtaaaagaa aatgaattaa atattgaata tattatcctt actcatgggc atggagacca
     4021 tataggatat gttaaggata ttaaagaaaa aactggtgct aaagtagttg cacatgttga
     4081 tgaaaaagaa ttattaaatg acaaaaataa aaatctaagt tatacaatga gatgtggagc
     4141 acaagagttt gatgcagata tctatgtaaa tgataaagac aagcttgaac ttggtgaatt
     4201 aaaaatgact ttcttgcata ctccaggaca tactcaagga tgtatgtgta taagagttga
     4261 agatgaaatg ttcacaggag atactttgtt tgctggaagt attggtagaa ctgatttata
     4321 tagtggagac tttaaccaaa tggaaaaatc acttaagaaa ttatgtaaat atgaagatgc
     4381 tgttagagta tatcctggac atggaccaac cagtactcta ggagttgaaa agaagacaaa
     4441 tccttacatg taattttata ttagtattta aaaaagtata aaatatttaa taactatatt
     4501 agtgaggggg ttcttctact gttaaataga aggagttaag ttgatgttgt atgttttttt
     4561 aaaaggacat gattataagt atgaagtagc agaacttata aaattgttta ctagtgaatt
     4621 taagtttaaa gactctaatg ctaacaataa tatagagtca aagcatttaa taaatagatt
     4681 gatttatgag aatggtgtat tattctcaag tactgaatac tatgaaaatg gaaacttaaa
     4741 gtacgagtca atacagaata tagaaaatat gaatcttgaa tttttagatg gatttgatag
     4801 cagcaaaacg attgaagaat ttcttaatag cctagatagt gaaagtaaga ggaactttaa
     4861 aaagctgtgt aaagaaaata tcaaaaaaag catgttttta gtgcttaaac aagtatttaa
     4921 ttcttatgta ccatggggaa ttttgactgg tattagacca gtcaaaattg tccacaattt
     4981 aatggataaa aagctagatg ataatagcat aagaaccatt ctaaaagata attattttat
     5041 aatagatgag aaaatagatt tagctcttga aatagctaaa agagagcgat tatttatgta
     5101 tcctatagat aaaaataaaa tttctcttta tgtaagtatt ccattttgtc caactagatg
     5161 tgtatattgt tcatttcctt caaattcatt aaaacaattt ggacatttaa aaagagaata
     5221 tgtttataaa ttgattgaag aaattaaggg gtttgccaaa gttattaagg atactaagaa
     5281 agaagttgaa actttatata ttggtggtgg aactccaact acacttgatg aggaagaatt
     5341 ggatttgctt ataaattcat tgttcaatga attagattta agtaaaatta aagaatttac
     5401 tgtagaagca ggtagacctg acactattac agaacaaaaa ctaagagtgt taaaaaaaca
     5461 caatgttagt agaattagta taaatcctca aactatgaat gatgatacct tggtaaagat
     5521 tggaagagag catagagtat ctgatttagt agattgcttt aatatggctc gtagaatggg
     5581 atttgataat ataaatatgg atataatttt aggtcttata gatgaagact taaatatggt
     5641 aaagaacaca ttggaggaga taaaaaaact ttctcctgaa agccttacag ttcatacatt
     5701 agccataaag agagcatcta acttaaatat aaatatggat aggtataaag aacatctaac
     5761 acaatatgaa gaaatggtaa aaatgataga tttatcaatg aaatatgcaa aagatatggg
     5821 attaaatcca tattatatgt acagacaaaa acatatgttg ggaaatttag aaaatatagg
     5881 ttatgctaaa gaaggatatg agtgcattta taatatacag attatggaag aaaaacaaag
     5941 taactatgca ttaggtgcag gtgctatatc gaaatttgtt tatgtagatg aagatagaat
     6001 tgaaagagta gaaaatgtaa aaaatgttga acaatacatt gaaagagtag atgaaatgat
     6061 aaaaaggaag aaagaagagg tatataaaaa tgttgactaa agctcctaga ggaactaaag
     6121 acataactcc aaaagaggct tacaaatggc gttatgtaga aaataaattt agagaaatat
     6181 gtgctttata tggatatgaa gaaatggtaa cacctatatt tgaacataca gaacttttca
     6241 agagaagtgt tggagatact acagatatag ttcaaaaaga aatgtattct tttaaagata
     6301 aaggtgatag agaaattacc ctaaagccag aaggtacagc aggtgtagta agagcattta
     6361 tagaaaataa attatatgca gatactcaac caacaaaatt attttatgtg acaccttgtt
     6421 ttagatatga aagaccacaa gctggtagac aaagacaatt tcaccaattt ggaatagaag
     6481 cacttggaag tgatactcct tcaatggatg ctgaaataat cgcactagct gttcaatttt
     6541 ttaatgaagt tggattaaat gatttggtag tgagtataaa ttcagttggc tgtccagttt
     6601 gtagaaaaga gtacaatgct ttattaaaag agtatcttga ctcaaaagct gatatattgt
     6661 gtgatacttg caatgagaga agagaaaaaa atccaatgag ggttatagac tgtaaaaatc
     6721 ctacatgtaa agaaaatatc aaagatatac catttattgc agaccatttg tgtgatgatt
     6781 gtaaatctca ctttgataaa cttcaagaat acttaaagga aatgaatata aactttgtaa
     6841 ttgataaaac aatagttaga ggcttagatt actatagaaa aactgctttt gaaattatat
     6901 ctaatgatat aggagctcaa agtactgtat gtggtggagg aagatatgat ggtcttgttg
     6961 aacaattagg aggaccaaaa ggtataagtg gaattgggtt tggactagga attgaaagac
     7021 ttttacttac acttgaaggt aatggaattg aaatagaaaa ccctcaatct acggatatat
     7081 ttatagtaac tataggtgaa gaagcaaata caagaagctt taaattgtta aaagacttga
     7141 gacaaaatca tataagtgca gataaagacc atatagagag aagtgttaaa gcacaattta
     7201 aatattcaga taaaataaat tcaaaattta caatagtaat tggagatgat gaacttaaaa
     7261 atgacactgc gacattaaaa aatatgaaaa catcagagca aactactgta aaattaagta
     7321 cattagttga agaattaaag caaaagctgt aaaataagaa agtatgatgt tttgtgtatt
     7381 agataaatat atagcattaa aattaaataa aatggaggta gtatcttgga aactttaaag
     7441 ggtttaaaga gaactcacta ctgtggagag ttgagagaaa aaaacataaa tgaagaagtt
     7501 gtacttatgg gatgggtaca gaaaaaaaga aatttaggtg gtcttgtatt tgttgattta
     7561 agagatacaa gtggattatg tcaaatagtt tttgatacag atgtagataa agaagctttt
     7621 gaaaaagctg aaaaattagg ggctgaattt gtaatagctg taaaaggaaa agtatgcgaa
     7681 agacaatcta aaaaccctaa tatgccaact ggtgatatag aaatatttgc aactgaactt
     7741 agacttttaa acaagtcaga aactccgcct atttatataa aagatgatga tgatgtttct
     7801 gaagcactaa gattaaaata tagatactta gatttaagaa aaccatctat gcaaagaaat
     7861 ttaaaattaa gacataaagt aatgaatatt acaagaaatt acttatcaaa caatagattt
     7921 tgcgaaatag aaacaccatt tttaatagct ccaacaccag aaggagcaag agattatcta
     7981 gtaccaagta gagttaatcc aggaaaattt tacgcattac cacaatcacc acaattatat
     8041 aaacaattat tgatggtaag tggtatggat agatatttcc aaatagttaa atgctttaga
     8101 gatgaagact taagagcaga tagacaacca gagtttacac aaatagactg tgaaatgtct
     8161 tttgttgagc aagaagatgt tatgagtatg attgaaggtt tgcttgaagc gatatttaaa
     8221 gaagttttag atgtagaatt ggcacttcca cttcctaaga tgacttatgc agaagctatg
     8281 tctaaatatg gttcagataa accagatact agatttggat atgagttaac agatatatct
     8341 gatgttgttt gtaactgtgg atttaaagtg tttgctgatg ctacacaacc aggaaaaagt
     8401 gttagaggta taaacgtaaa aggcaaagct gacgacttta ctagaaaaca aatatcatca
     8461 ctagaagaac atgctaagac atatagagca aaaggtcttg cttggatgaa agtaggacaa
     8521 gaaggagtaa cttctccaat agcaaaattc ttcaatgaag aagaaatgaa cgctatactt
     8581 acaagaatga atgctgaagt aggagattta cttttatttg tagctgataa aaactcaata
     8641 gtatttgatg ctttaggaca agttagactt gaagtagcaa ataggcttaa tctattggat
     8701 aaaaatgtat ataacttact atgggtaact gagttcccag tttttgaaga agatgaagaa
     8761 acaggcacat tctcagcaat gcatcaccca tttacatcac ctatggatga agatttagac
     8821 aagttagaag aaggtgataa gtcatcttta agagcgaaag cttatgatat tgtattgaat
     8881 ggttatgaaa taggtggagg aagtgttaga atatcaaatt ctgatgttca atcaagaatg
     8941 tttaaagcac ttggttttac agaggaaaga gctaatgaaa agtttggtta tttattagaa
     9001 gcatttaaat atggtactcc tcctcatgct ggtctagctt ttggtcttga tagacttgtt
     9061 atgttacttg caggaactga taatataaga gaagttattg cattccctaa aaatcaaaat
     9121 gctgtttgcc ctatgacaaa tgctcctaca ttggcggaag atgagcaatt agaagaatta
     9181 agtataaaag ttgatataaa agataatgaa taatatgtag ttgcaagaaa ttaaaatgta
     9241 aaaagtctgg atataaagta tatttatatt cagacttttt ttattagaat tattttatat
     9301 tttttctcta tatattttca taaattatac ttgtattctc aaccaatata attaaaatac
     9361 tatgtttaaa aattttacat aaacttggtt gttttagaaa ctattttttt aatgagtaca
     9421 cgattttaat taaagaatat ataacaaatt tgaaataagt ctaatatatg acttaataga
     9481 ttaaattaaa ataataaatt tggacgattt atctatttat aacttaaatt atatcaaatt
     9541 gataacaaac tagatattat acttttttgt agaatagaat tacaattata taaagaaaaa
     9601 ttctattaat agtgaaaaaa aagtagaaaa aagtagaaat tattctttta taaagaaagg
     9661 gagggataat attgcaagag agagaagaat tagttttaaa tccagttcca aaagaggaaa
     9721 gagttggatg gttagctcca ttgtttaata tgcttggttc taacatagct atatctgaac
     9781 taatggttgg gggaacttta attttaggaa tgactctttc taatatgatt attacatcaa
     9841 ttatagggaa cttaatatta gttgcaatta ttatgataca aggttatata ggttacaaag
     9901 aagggttaaa tacgtatgta ttagctaagg gggcatttgg agagatagga ggaaagtatc
     9961 ttatatctct attacttggt ataactagtt ttggatggtt tggtgtacaa gcaggggttg
    10021 caggactatc agtacaaaag atattcccaa gtgtaaacct aacattagtt acagttatac
    10081 ttggattatt tatggttgtt tttgcactgt atggttttaa agctatggct aaatttaatt
    10141 acttagttat accaccactt atgatattaa tggtgtgggg agtttttaaa gctttttcta
    10201 cttatggagt tgaagctatt tataattata ctcctcaaac aactatgagt atggttgaag
    10261 gtcttaatat tgtagtaggt cttgttatag ttggtgcaat tatatcacct gaccagttaa
    10321 gatatacgag acgagtaaaa gacatttgga ttatcagttt tttaggtcta gggattattt
    10381 cattattcca acaagttgca gctggtgtaa tgtctatggg agcacctact tgggatataa
    10441 cagaagtatt ggctaattta ggttttggat gggtagcgtt tgttatttta atacttgcat
    10501 cttggtctac aaacgtatca aatgcttatt ctggtggatt agcattaaaa actatttttc
    10561 caaatgtaaa aagaaatgtt ttgactttag ttgcaggtct aataggtaca ataatagcag
    10621 ctactggtat aatatttaaa tttcagtcat ttttatcttt cttgggaatc gcagttcctg
    10681 caatagcagg aattatgtgg tgtgagtatt attttataca aggaagaact tataagcata
    10741 gagaaggaat aaattggatt gcagtaataa gctggttaat aggttttgca gcgtcgtatt
    10801 atagttcaaa aataaatttc ttaatacctc cgataaatgg aattgtagtg agtatggtta
    10861 tttattatat attaatgaat tgttttggaa tcaaagataa ataaaggggg caattacatg
    10921 ggtcgtaagg tgaaaatagg tataattcaa caacacagtg tacttggaaa tgttaaaaaa
    10981 aatatagaaa aggcagtaga gatgatagat gacttaggaa aacaaggggc agatataatt
    11041 tgtttaccag aactatttgc tacaggatat aatttagagt ctttaggtgg agttaaaaca
    11101 ttagaattaa taagagaaca taataagtac atagaggaat caatgtcaga agctgctaaa
    11161 agaaataatg tatacctgat atctccatat ggaaccttag aaaaagggtc tacacatgtt
    11221 tataattcag cagttatatt tgatagaaaa ggtaagatta tgggagaata ctgtaaaaat
    11281 catctgtggt cattagaagc agtatacttt aaaggagggg aaaaagtaga ggtatatgat
    11341 gctgattttg gtagatttgg tgtaatgata tgttatgatg caggttttcc agaagtatcc
    11401 agagaattga cattaaaagg ttctgaaata atatttatac catctgcatg gagaatacaa
    11461 gatgaagata tgtgggattt aaatgtatct caaagagctt tagagaacac tgtatataca
    11521 gtaggtgtaa atttagtaag taatgactct aatttaatac tatttggaaa gagtaaaata
    11581 tgcaatccta gaggtactgt aataactcaa ttagatacat acagggaaaa atatgtatta
    11641 tcagagattg atttagatga aataaaaaag tatagggaag atatccccta tttgaaagac
    11701 agaaaaagta gctataacat ataattctat actataatac aagttcaata taaaatgata
    11761 aaaagaggat gtatcaatat taagattaaa tttattatac tttgatatat cctctttaat
    11821 attatcaact ttgatttaaa tatttttaaa atagttgata agaaagtttt tgaacaacaa
    11881 cacagcttta gattcatagt tttcgctaaa ccatgaaaga tttaaatatc ttttaaattc
    11941 aatatctttg atatgtaaca acttaatctc agggtcttga gataatcccc atgattttcc
    12001 acatataaat ccaactcctt gaagtgattt tataagagca taaattgtat atggtgaatc
    12061 agactcaaat gctattttgg gtttgaaatt agctgattcg cacaggatat caattacctc
    12121 tctataattc tcacccttcg ttataactat aaagttttca tctgaaactt cgcttagata
    12181 tacttcatcc tttaatgata aaggatggtt tattgaaact cctaatagta tttcttcgca
    12241 tgttaaagtt atgctatttt ctgagtttag ctttgtaaat gaagaagata tgtacaaatc
    12301 aaaatcactt tttttataag atgatggcaa agtatgtgac acattaaaag taatattggg
    12361 atatagtttt ctgaaattac tcaaaagtga tggtaacaca ttagccgctg caggagataa
    12421 tatctttact tctcctgaat tttccaaaga agtgtcttgt aactcctttt ttgaactttc
    12481 tactaaatct aaaatgctat ctactctttt tagaaacaat cttccatact catttaaaac
    12541 aatatatctt ccttttctgt caaataactg aactccaagt tctttttcta aggaagatat
    12601 agttttgctt aaagctggct gtgctatatt taacttgtgt gatgcttttg ttatgtgctc
    12661 cattctagca acagtttgaa aatacttaag atgcaataaa tccatagtat aaaatacacc
    12721 tcttttaata tgacttgagt tatatcaatt ttataatcaa ctccacatta tacattataa
    12781 tataaagttt atataataaa ctacaatata ttaaaaagtt atttaaattg ttatttatat
    12841 tttgtacata atctaattaa aaaaagtaac tttaaatg
//
LOCUS       DBGJFF010000110        12848 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.111, whole genome shotgun sequence.
ACCESSION   DBGJFF010000110 DBGJFF010000000
VERSION     DBGJFF010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12848
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.111"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(122..646)
                     /locus_tag="ACOIAF_002948"
     CDS             complement(122..646)
                     /locus_tag="ACOIAF_002948"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HGZ2669949.1"
                     /translation="MENKNPIVTIEMENGKEIKIELYPNIAPNTVKNFVSLVNEGYYN
                     GIIFHRVIPGFMIQGGCPNGTGMGGPGHSIKGEFSGNGFTNNLKHERGVISMARTMAP
                     NSAGSQFFIMHKNSPHLDGQYAGFGRVIEGMDTVDEIASVRTDSADKPQTPQVMKSVT
                     VETFGIDYSDVEKN"
     gene            complement(711..1463)
                     /locus_tag="ACOIAF_002949"
     CDS             complement(711..1463)
                     /locus_tag="ACOIAF_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HGZ2669950.1"
                     /translation="MKLGLCLEGGGAKGAYQAGVVKALYDGGINKFYSISGTSIGAIN
                     GYYLYTGNVDNLEKMWTNIKDIQNGNVKIVNNTVDNSPAIDNLRELDDSNIEEMNFYV
                     NYVEVDNKVVSEKIVDVSKMPRNEAIISISYSGLLPSNPNATLGFKEQFVKDVQEGIY
                     DGFKLDGGLIRSALIEPLIGDNVDKIILISTKYNYELPEDIKKVYDEDKIIVVRPNTQ
                     FAPKDTLNFDDEFCKTIYQEGYEIGKNILDRL"
     gene            complement(1494..2792)
                     /locus_tag="ACOIAF_002950"
     CDS             complement(1494..2792)
                     /locus_tag="ACOIAF_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HGZ2669951.1"
                     /translation="MDSINFAKNLIDFIYDSPSSFHAVESTKEILDKNGFEELVLNQR
                     WNLRVGGKYYVTKNLSAIVAFVVNSEGIERDGFRIIGSHSDSPTFSIKPNAEIEAEKS
                     YLKLNTECYGGAILSTWLDRPLGIAGRVVLKGDSILKPNERLIDFKKPICIIPNLAIH
                     LNRSINDGHSYNKQKDMLPLVGMLNDTLEKDNFLIKQIASNLDVNMDDIIDFDLFLYE
                     FEKGSLIGANDEFISIGRQDNLAMVHASLNALVNTKGQHGVNVMAVFDNEEVGSSTKQ
                     GADSNMLLNILERICISMGKDREGFFESIYSSFMISSDLAHAIHPNIPEKHDPTNKPI
                     MGKGPVIKINASQAYTSDGYSIAIYKNICREANVEYQEFVNRSDERGGSTIGPISSTH
                     IDIPSVDVGSPILAMHSIRELGNVEDHYSIYKTFSKFFEI"
     gene            complement(2809..5073)
                     /locus_tag="ACOIAF_002951"
     CDS             complement(2809..5073)
                     /locus_tag="ACOIAF_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3553 domain-containing protein"
                     /protein_id="HGZ2669952.1"
                     /translation="MNLDTLNPAQREAVEKTEGPVLILAGAGSGKTKVLTTRIAYLIE
                     DKGVQAPNILAITFTNKAANEMRERVEQNIGPETKDMWISTFHSCCVRILRKDINKIG
                     YNRSFVIYDSADQVTLVKDCLKELNLSDKVFEPKAVISAISGAKDKLYTPKQFKDINM
                     ADNRMVKIADIYALYQDRLKRNSALDFDDLILKTVELFKANDEVLAYYRSRFRYIMVD
                     EYQDTSKAQYELIKLLAREHQNICVVGDDDQSIYGWRGADIRNILEFEKDYDNVHVVK
                     LEQNYRSTQVILDAANKVISNNIERKRKKLWSEKKEGELIKIQLTGSEIEEADFIADS
                     IAQIARKENRPYKDFAVLYRANAQARPVEDALNRSQIPYNIYGGTKFYERKEIKDLLA
                     YLRVIQNPQDDISIKRIINVPRRGIGLRTIEKIEDRANLKQESIYSVLIDIETNSDIS
                     TKARASISGFVDIIGTLRTIKEVYPVSKLIEKVLDTTGYMDELVEIRNKNEKDLTGKG
                     EEAQDRIDNLREFISIALEFESSNDDTYENKDLETFLTSIALTSESNDEEDNDRVSLM
                     TIHTSKGLEFPVVFLTGMEEGLFPISRAIKSMSDSQIEEERRLCYVGITRAKEELYMS
                     LTEKRTLYGKTNVAIASRFMEELPEECIERLYKVKKELSYSKASYNMLDKYTKKYMST
                     ISKSKVADKVNATIKDSNKETNPDDIKLGSKVHHPKFGVGTVVSIIGTDVTIAFDQQG
                     IKKINKEYTTLNIL"
     gene            complement(5328..6146)
                     /locus_tag="ACOIAF_002952"
     CDS             complement(5328..6146)
                     /locus_tag="ACOIAF_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HGZ2669953.1"
                     /translation="MFKINNLTYQYEKNTNALLNINMDFSKGNVIGIIGSNGSGKSTL
                     FMNLMGILKPTSGEILFKEEKLKYDKRSLYNLRKNVGIVFQDPEKQIFYSKVYDDIAF
                     AMRNIGMDEKTIKERINKALVAVNGIDFIDRPVHFLSYGQKKRVAIASVIAMENEIVL
                     LDEPTAGLDPVSTRSIVDIIKGLNKNNIKIVISSHDMNLMYEICDYIYVLDKGILIDE
                     GKAENVFINENNIIQAGLESPWLVKVHRNMNLPLFKKEEDLYKYWKERELNTNK"
     gene            complement(6139..6837)
                     /gene="cbiQ"
                     /locus_tag="ACOIAF_002953"
     CDS             complement(6139..6837)
                     /gene="cbiQ"
                     /locus_tag="ACOIAF_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HGZ2669954.1"
                     /translation="MLIIDKYAYTNRLSKVNPNKKVAIGVIFLIASMVIQNIFILSGI
                     MILMSILVVCVAGIDLKNYLKLLRIPMYFLFLSIGITLVNISFNKADLLYSFEFFSFN
                     VGISKASIDMSIHVLFRAMSCLTCVYFCILTTPFNQLIFFFKKLHLPDTFVELSMLIY
                     RFIFIFLEEFSEIYKSQELRFGYINLKTSYKSLGILGSMLYKRLMTRYDDMCISLDIK
                     LYDGKFHIVGDNDV"
     gene            complement(6840..7124)
                     /locus_tag="ACOIAF_002954"
     CDS             complement(6840..7124)
                     /locus_tag="ACOIAF_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     substrate-binding protein"
                     /protein_id="HGZ2669955.1"
                     /translation="MSAKTKTKTNILLLLLVVALIIFPLLVNSGAEYGGADGQAESEI
                     TKINPNYKPWFSSPYEPPSGEIESLLFSAQAALGAGVIGYILGLQKGKRS"
     gene            complement(7114..7866)
                     /locus_tag="ACOIAF_002955"
     CDS             complement(7114..7866)
                     /locus_tag="ACOIAF_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0000041 - transition metal ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HGZ2669956.1"
                     /translation="MKQNIKLGVIAALMLIILTPVTSNAMHIMEGYLPVKWSIAWGVI
                     FIPFFLFGLKSIGKIVKQDPKKKVLLALCGAFVFVLSALKIPSVTGSCSHPTGVGLGA
                     IMFGPSVMFVLGTIVLIFQALLLAHGGITTLGANAFSMAIIGPIVSFLIFKALKKKDG
                     NNAIPVFLAAAIGDLATYTITSIQLALAFPDPSGGVMASAIKFLGIFFMTQIPIAIAE
                     GILTVIVYNLITENGEKSILENNDKGVKANEC"
     regulatory      complement(8048..8240)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(8372..10144)
                     /locus_tag="ACOIAF_002956"
     CDS             complement(8372..10144)
                     /locus_tag="ACOIAF_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation-like protein"
                     /protein_id="HGZ2669957.1"
                     /translation="MKWIYDDYEFENRANNLSWTIAGDYNESIDISEKDYSSKEIGLY
                     FAIIAGARRKYVDWDIVKKYLTNRIRKGYNKDIILGLIQVILNTIVEEKVIKDRPGIE
                     DIRRNAYKDIVSRFTKIHNDNIMDKIKYTFVLYSMDKHPALDSMTRRIVNDIRNIDTN
                     QDIMEILKALDVIYLTYFEYILNGEENSLIDENNVESVEVNFDTFADFMYEELYSDEE
                     ENIIESDIDSISSSMLVEGVGDFDDSKCSNSADRVIYVDEDTANKIYSKIEHYYGKTY
                     LSKGEIKKIETQNCRNVHEGCRIHFTDGVLRSECNNVFQLKYVTRQKENNLGKFREHA
                     RMYRQQIKRLKESLSRILIEENAKSRVYSDCGTILANRAWRVGRSNNNKIFYKDIENE
                     KGKYVIDILLDASGSQSRNQGNVAIQAYIIANALTEVGIPNRVMGFSSFLDYTILKRF
                     KDYDDSIKNSENIFEYFCAGNNRDGLAIKSICSGLINRDEENKILIVLSDGRPNDVKI
                     GKSSERTLRGEKAYRGIVGLRDTANEVRKARKQNILVLGVFTGKESELEAERLIYGND
                     FIYSRDITRFSDVVSMYLKKIIRN"
     gene            complement(10158..11087)
                     /locus_tag="ACOIAF_002957"
     CDS             complement(10158..11087)
                     /locus_tag="ACOIAF_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HGZ2669958.1"
                     /translation="MDIIDNLRAQGVDEKLIEDVMYFRNYYGLEKDLEYRVTKSKTYF
                     YGKDILSMCIAAILEEENILLSGPKATGKNLLADNLGEIFNRPQWNTSFHINTDSSTL
                     IGTDTFIDNEVKLRRGSVYECAINGGFGVFDEINMAKNDAIVVVHSALDYRRIIDVPG
                     YERVNLHPATRFIGTMNYEYAGTKELNEALVSRFMVIDIPPIEEDKLMMILKNEFSDA
                     DEEKLIQFAGIFLDLQLKSQNGEISSKAIDLRGLMASLKTIRRGLKPTLAINMGLTGK
                     TFDAYEKEMVGDVIKTRIPNKWESIDVFPISHI"
     gene            11551..11703
                     /locus_tag="ACOIAF_002958"
     CDS             11551..11703
                     /locus_tag="ACOIAF_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669959.1"
                     /translation="MKKIKKLIILIVFLFLFGMLHCYTEWKFNTDPPKGIIERINEWT
                     DKIGLD"
     gene            complement(12075..12683)
                     /locus_tag="ACOIAF_002959"
     CDS             complement(12075..12683)
                     /locus_tag="ACOIAF_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HGZ2669960.1"
                     /translation="MSVINSFILQYGLISVFVLIMIEYACFPLPSEVVLPLCGAIAAR
                     NHFGFLTILILSIIAGILGSIFCYTVGNWGGKSIINKIIEICPKAKKGIFASQDYFNK
                     YSSISVCVCRLIPLCRTYISFIAGIAGQNIITFVVSSIVGITIWNTSLISIGYIFSES
                     WVRIMSHYNDYKFLVLIIPISIIFIGFVIKSFIHKKHHKIRL"
BASE COUNT         4129 a         2306 c         1348 g         5065 t
ORIGIN      
        1 ataaattaaa aagtgaaatg tatttgtcta aaaacataat aacatttcac tttatcctca
       61 tttttaagta atatatattt ttaattttta tttataacta aatctagttt ttatataaat
      121 tttagttttt ttctacatct gagtaatcta ttccaaaagt ttctacagta acagatttca
      181 taacttgtgg agtttgtggc ttatcagctg aatctgttct aactgatgct atttcatcaa
      241 ctgtatccat accttctatc actctaccaa aacctgcata ctgaccatct aagtgtggtg
      301 agtttttatg cattatgaaa aattgagaac cagctgaatt aggtgccata gttctagcca
      361 ttgatatgac tcctctttca tgttttaaat tgttagtaaa tccattacca ctaaattcac
      421 cttttattga gtgacctgga ccacccattc ctgtaccatt tgggcatcct ccttgtatca
      481 taaatcctgg tattactcta tgaaatatta ttccattgta atatccttca tttactaatg
      541 atacaaagtt tttaactgta tttggagcta tatttggata taactcaatt ttgatttctt
      601 ttccattttc catttctata gttactatag gatttttatt ttccataatt tattaccttc
      661 cttttcatta gttaaattac ataattatat ttattatatt atactctaaa ttaaagtcta
      721 tcaagtatat tttttcctat ttcatatcct tcttgataaa tagttttaca aaactcatca
      781 tcaaagttaa gcgtgtcttt aggagcaaat tgagtattag gtctaactac tattatttta
      841 tcttcatcat atactttttt aatatcttct ggcaactcat aattatattt tgtggatatt
      901 aaaataattt tatccacatt atccccaatt aatggttcaa ttaatgcact tctaattaaa
      961 cctccatcta atttaaaacc atcataaatt ccttcttgca catctttcac aaattgttct
     1021 ttaaatccaa gagttgcgtt tggatttgat ggtaacaacc cactgtatga aatgctgata
     1081 atggcttcat tacgaggcat ttttgatacg tcaacaatct tttctgatac tactttatta
     1141 tcaacttcaa cataattaac ataaaagttc atttcttcta tattactatc atctagttcc
     1201 cttaggttgt ctatagcagg agaattatcc acagtattat ttactatttt tacatttcca
     1261 ttctggatat cttttatatt tgtccacatt ttctccaagt tatcaacatt ccctgtgtac
     1321 aaataatatc catttattgc tcctattgat gtccctgaaa tcgaataaaa tttatttatt
     1381 cctccatcat acaaggcttt aactactcct gcttgataag ccccttttgc tcctccacct
     1441 tctaaacata atcctaattt catagtctca tctccaaatt gtttatctta tatttatatt
     1501 tcaaaaaact tactaaatgt tttatatata ctatagtggt cctcaacatt tcctaattct
     1561 cttatagagt gcattgctaa aattggactt cccacatcca cagatggtat atctatatga
     1621 gtagaagata ttggacctat agtacttcct cctctttcat ctgacctatt aacaaattct
     1681 tgatactcaa cattagcttc tctacatata tttttataaa tagctataga atatccatca
     1741 ctagtatatg cctgactagc atttatcttt ataaccggac cttttcccat tattggttta
     1801 ttagttgggt catgcttttc tggaatgttt ggatgaatag catgagctaa atctgatgat
     1861 atcataaaag atgaataaat agactcaaag aatccctctc tatccttacc cattgatatg
     1921 caaattctct ccaatatatt taacaacatg tttgaatctg caccttgttt agtagagcta
     1981 ccaacttctt cattgtcaaa tacagccatt acatttacac catgttgacc tttagtatta
     2041 acaagagcat tcaaactagc atgtaccatt gcaagattat cttgtctccc aatagatata
     2101 aattcatcat tagcccctat taaacttcct ttttcaaatt catataaaaa taaatcaaaa
     2161 tctataatat cgtccatatt tacatctaag ttagatgcta tttgctttat aagaaaatta
     2221 tctttttcta atgtatcatt taacatccca actaaaggta acatatcttt ttgcttatta
     2281 tatgaatgac catcatttat acttctattt aaatgtattg ctagatttgg tattatacaa
     2341 attggctttt taaagtctat aagcctttca tttggtttta atatactatc accttttaaa
     2401 actactctac cagctatccc aagaggtcta tccaaccatg tacttaatat tgctcctcca
     2461 tagcattctg tatttaactt caaataagat ttttcagctt ctatttctgc atttggttta
     2521 atactaaatg taggagaatc tgaatgagag cctattattc taaatccatc tctttctata
     2581 ccttctgaat ttactacaaa agctactata gctgataaat tcttagtaac ataatacttt
     2641 ccaccaactc ttagattcca tctttgattc aaaactaatt cttcaaatcc atttttatct
     2701 aagatttcct tagtactctc gactgcatga aaagacgacg gactatcata tatgaagtct
     2761 attaagtttt tagcaaagtt tatactatcc attcctcatt tacctccatt ataaaatatt
     2821 tagtgttgta tattctttat ttattttttt aattccctgt tggtcaaaag ctatagtaac
     2881 atctgtacca attatagaaa ccactgtccc aaccccaaat ttaggatgat gtaccttaga
     2941 accaagtttt atatcatctg gattagtttc tttattagaa tcttttattg tagcattaac
     3001 tttatctgca actttagatt tgcttatagt actcatatac tttttagtat atttatctaa
     3061 catattataa cttgctttag aatagcttag ttcttttttt actttgtaca atctttctat
     3121 acattcttct ggcagttctt ccataaatct tgaagctatt gcaacatttg ttttaccata
     3181 taatgttctc ttctctgtca atgacatata tagctcttct ttagctctag ttataccaac
     3241 ataacaaagt cttctctctt cttctatttg tgaatcactc atagatttta tagctcttga
     3301 tataggaaat aacccttctt ccatacctgt taaaaacaca acagggaact ctaaaccttt
     3361 ggatgtatga attgtcataa gcgaaaccct atcattgtct tcttcatcat ttgattcaga
     3421 tgtaagtgct atacttgtta ggaatgtctc taagtcttta ttttcataag tatcatcatt
     3481 actactttca aactccaaag ctatagaaat aaattctctc aaattatcaa ttctgtcttg
     3541 agcttcttca cctttgcctg ttaaatcttt ttcattttta tttcttatct ctacaagctc
     3601 gtccatatat cctgtagtat ccaagacttt ttctataagt ttgctaacag gataaacttc
     3661 ttttatagtt ctaagagtcc ctataatatc aacaaatcca cttatacttg cccttgcctt
     3721 agtagatata tctgaatttg tttctatatc aattaatact gagtatatac tttcctgttt
     3781 taagttagct ctatcttcaa ttttttctat agttctaagc cctatacctc ttctaggaac
     3841 atttataatc ctttttatag atatatcatc ttgtggattt tgaataactc ttaaatacgc
     3901 caacaaatcc tttatttctt ttctctcata gaattttgta ccaccatata tattgtatgg
     3961 aatttgactt ctatttaaag cgtcctctac aggtcttgcc tgtgcatttg ctctataaag
     4021 tactgcaaaa tccttataag gtctattttc ttttctagct atttgtgcaa ttgaatcagc
     4081 aatgaaatca gcctcttcta tttcactacc agttaattgt atctttataa gttctccttc
     4141 ttttttttca ctccaaagct tttttctttt tctttctata ttattagata taactttatt
     4201 agctgcatct aatatcactt gtgtagacct ataattttgt tccaacttta caacatgtac
     4261 attgtcataa tctttctcaa attcaagtat gtttcttata tctgcacctc tccatccata
     4321 aatactttgg tcatcatctc caacaacaca aatattttga tgttctcttg ctaataattt
     4381 tatcaattca tattgagctt tacttgtatc ttggtactca tctaccatta tgtatctaaa
     4441 tctacttctg taatatgcta acacttcatc attagccttg aaaagttcta cagttttaag
     4501 tattaaatca tcaaagtcaa gtgcactatt tctttttaaa cggtcttgat atagcgcata
     4561 aatatctgca atcttaacca ttctattatc tgccatattg atatctttaa actgctttgg
     4621 agtataaagt ttgtcttttg ctcctgaaat tgcacttata actgcttttg gttcaaacac
     4681 tttatcactt aaatttaatt cttttagaca gtctttaacc aatgtaactt ggtctgctga
     4741 atcataaatt acaaaacttc tattgtaacc aatcttattt atatcttttc ttagtatacg
     4801 aacacagcaa gaatggaatg tacttatcca catatcctta gtttcaggac ctatattttg
     4861 ttcaactctc tctctcattt catttgcagc tttgtttgta aatgttatag ccaatatatt
     4921 tggtgcttgt acacctttat cttctataag atatgctatt cttgttgtta ataccttagt
     4981 tttacctgaa ccagcaccag ccagtataag tactggacct tcagtctttt ctactgcttc
     5041 tctttgagca ggatttaatg tatctaaatt catatttctc ctcctgttaa cagccataaa
     5101 tatattttaa tctaaaatat gaattacttc aaattataat tatgtattat attaatagat
     5161 tatttccaat atattacaat taaataacat ttttccatat aatttatttc attgtttaat
     5221 aaaaaaaata accttatgat ttggggaatc ataaggcacc tttaaggggt ctaggttaag
     5281 ggttattata tataaatcta tttttttaaa ataggtttag ggcttaatta tttatttgta
     5341 ttaagttctc tttccttcca atacttatac aaatcttctt ctttcttaaa taagggtaaa
     5401 ttcatatttc tatgtacttt tactaaccat ggggattcta ggccagcttg tataatatta
     5461 ttttcattta taaacacatt ttctgccttt ccttcatcaa tcaaaatccc cttatccaat
     5521 acatatatgt aatcacatat ttcatacatt aaattcatat catgactgga tattactatt
     5581 tttatattat ttttatttaa tccctttatt atatctacaa tagacctagt acttactggg
     5641 tctaaaccag ctgtaggttc atcaagtaat acaatttcat tttccattgc tataactgat
     5701 gctattgcca ctcttttttt ctgtccatag ctaagaaaat gtactggcct gtctataaaa
     5761 tctattccat taacagctac tagagctttg tttattctct ctttaattgt tttttcatcc
     5821 ataccaatgt ttctcattgc aaaggctata tcatcatata cttttgaata aaaaatttgt
     5881 ttttctgggt cttggaacac aatacctaca ttttttctaa ggttgtataa acttctctta
     5941 tcatatttta acttttcttc tttaaaaaga atttctccag atgtaggctt taatataccc
     6001 attaaattca taaataatgt tgattttcct gaaccatttg aacctattat acctataaca
     6061 tttccctttg aaaaatccat attaatgttt aatagtgcat tggtattttt ttcatattga
     6121 tacgttaaat tatttatttt aaacatcatt atcccctact atgtgaaatt taccatcata
     6181 caattttatg tctaatgata tacacatatc atcatacctt gtcataagtc ttttgtacag
     6241 catacttcca agaataccta aggatttata tgaagttttt aaatttatat atccaaatct
     6301 tagctcttgt gacttgtata tttctgaaaa ttcttctaaa aatatgaata taaacctata
     6361 tattaacatt gataactcga caaaagtgtc aggtaaatga agttttttaa aaaagaatat
     6421 taattgatta aaaggtgttg taagtataca aaaatacaca caagtcaaac aagacatcgc
     6481 tctaaataac acatgaatag acatgtcaat agacgccttt gatattccta cattaaaact
     6541 aaaaaattca aaactatata ataaatctgc tttattaaaa gatatattta caagagttat
     6601 tcctatacta agaaataaaa agtacattgg tattctaagt aattttaaat aattttttaa
     6661 atctattcca gccacacaaa caaccaaaat actcattaaa atcataattc cacttaaaat
     6721 aaatatattt tgtattacca tagaagcaat cagaaaaatt actcctatgg ctactttttt
     6781 gtttggattt actttactca atctatttgt atatgcatat ttatcaatta taagcatgat
     6841 taacttctct ttcctttttg aagacctaat atgtatccta ttacaccagc ccctaaagct
     6901 gcttgtgcag agaataataa actttctatt tctccacttg gtggctcata tggactgcta
     6961 aaccatggtt tgtaatttgg atttatctta gtaatttcac tttcagcttg accatctgct
     7021 cctccatatt cagcacctga gttgacaagt aatgggaata taataagagc tactactaat
     7081 aaaagtaata atatgttagt ttttgttttt gttttagcac tcattagctt taactccttt
     7141 atcattattt tctagtatgc ttttttctcc gttttctgta attaaattat atacaattac
     7201 agtaagtata ccttctgcta ttgctattgg tatttgagtc ataaagaaaa ttcctaaaaa
     7261 tttaattgct gaagccatta ccccacctga tgggtctggg aaagccagtg ctaattgtat
     7321 tgaagttatt gtatatgtgg ctaagtctcc tattgctgct gcaagaaata ctggaatagc
     7381 attatttcca tcttttttct ttaaagcctt aaatattagg aatgatacga ttggccctat
     7441 tatcgccatt gaaaaagcat tagctcctaa agtagttatt cctccatgtg ctaataacaa
     7501 tgcttggaat attaaaacta tagttccaag aacaaacatt acgcttggtc caaacattat
     7561 tgctccaaga cctacaccag taggatgtga acaacttcct gttactgaag gtatttttag
     7621 tgctgataat acaaacacaa atgctccaca taaagctaat aatactttct ttttagggtc
     7681 ttgctttact attttaccta ttgattttaa tccgaataag aaaaatggta taaatataac
     7741 tccccatgct atagaccatt ttactggtaa gtaaccttcc attatatgca ttgcatttga
     7801 tgtaactgga gttaaaatga ttaacataag tgcagctata acccctaatt taatattctg
     7861 tttcatctta gtatccctct ctccctttct aataattaaa acataataat acatgtctga
     7921 tttatttaat aaaataaaaa agcctaactc gatggttagg cttctaattt tccacaatct
     7981 aaatacaaac acataaaaca aacatatctg tataattatt ataagaaact ttctccatcc
     8041 ccgaagaaat tactttaata ttttaggcag gtctcctgac ttagcttcat cctactcctc
     8101 ttccttccca tgagatataa aaaatatcat catagtggct aacaaaagtt tcgtctgcat
     8161 cacagtagcg ggggctgtaa aggaatttaa cctttttccc tattaatctt gcttaaagaa
     8221 cctataatat tatatattta atttttaaat tattcttaaa tcattttcta tataaatcat
     8281 tgtatacctt ttctgtgaaa atttctacat tttttataaa ttttattcat ttttatattt
     8341 ttatgtatat aaattctttt ttgtaagttt attaatttct aattatcttc tttaaataca
     8401 ttgaaactac atcagaaaat ctagttatat ctcttgaata tataaaatca tttccatata
     8461 ttaatctttc agcttcaagt tctgattctt ttcctgtaaa tactcctaat actaatatat
     8521 tttgctttct agcttttctc acttcattgg ctgtatctct caaaccaact atacccctat
     8581 aagccttttc tcctcttaat gttctctcac tactttttcc tatctttaca tcatttggtc
     8641 ttccatcgct gagaactatc aggattttat tttcttcatc tctatttatt agaccactac
     8701 atatagattt tattgccaaa ccatctctat tattaccagc acaaaaatat tcaaatatat
     8761 tttcactatt ttttatacta tcatcataat ctttaaatct ttttaatatt gtatagtcta
     8821 aaaaactaga aaatcccatc actctatttg gtatgccaac ttctgttaga gcatttgcaa
     8881 ttatgtaagc ttgaattgcc acatttccct gatttctact ttgagagcca cttgcatcca
     8941 ataatatatc aataacgtat ttcccttttt cattttctat atctttataa aatattttat
     9001 tattattact tctaccaact ctccaagctc tatttgctaa tatagtacca caatcagaat
     9061 atactctact tttagcattt tcttctatta aaattcttga cagactttct tttaatctct
     9121 ttatttgctg tctatacatt ctagcatgtt ctctaaattt tcctaaatta ttttcttttt
     9181 gtcttgttac atattttagt tgaaatacat tattgcactc acttctaaga actccatctg
     9241 taaagtgtat tctacaacct tcatgaacat ttctacaatt ttgtgtctca atttttttaa
     9301 tttcaccttt actcaaataa gtttttccat aataatgttc tatttttgaa tatatcttat
     9361 ttgcagtgtc ttcatcaaca tatataactc ggtctgctga attgctacat ttagaatcat
     9421 caaaatcccc tacaccttct acaagcatgg atgaagatat tgaatctata tcactttcaa
     9481 taatattttc ttcttcatca ctataaagtt cttcatacat gaaatcagca aatgtatcaa
     9541 aatttacttc tacactttct acattatttt catcaataag actattttct tctccattta
     9601 atatgtattc aaaataagtt aaatatataa catctaatgc ttttaaaatc tccataatat
     9661 cttgattggt atctatattt cttatatcat taactattct tctagtcata ctatctaatg
     9721 ctgggtgctt gtccatacta taaagtacaa atgtgtattt aattttatcc ataatattat
     9781 cattgtgtat tttggtaaaa cgtgaaacaa tatctttata agcatttctt cttatgtctt
     9841 ctatccctgg cctatcttta atgacctttt cttcaactat tgtatttaat atgacttgaa
     9901 ttagacccag tataatatct ttgttgtatc cttttcttat tctatttgta agatattttt
     9961 taactatatc ccaatctaca tactttcttc tagcacctgc aattattgcg aaatatagac
    10021 ctatttcttt tgaagagtaa tctttttctg atatatctat actttcattg taatcaccag
    10081 ctattgtcca tgataggtta ttagctctat tttcaaattc ataatcatca tatatccatt
    10141 tcataatctc acctatatta aatatgactt attggaaaca catctatact ctcccattta
    10201 ttaggtattc tagttttaat tacatctcca accatttctt tctcatatgc atcaaaagtc
    10261 tttcctgtaa gccccatatt aattgcaaga gttggtttca atcctcttct tatagtctta
    10321 agagatgcca taagaccacg taaatcaata gctttactag atatctcacc attttgagac
    10381 ttcaattgta aatctaaaaa tattcctgca aattgtatta atttttcttc atcagcatct
    10441 gaaaattcat tttttaaaat catcattaac ttatcttctt ctataggagg aatatctatt
    10501 accataaatc tagataccag tgcttcatta agttcttttg ttcctgcata ttcataattc
    10561 atagtcccaa taaatcttgt tgctggatgt agattgactc tctcataccc aggtacatca
    10621 attattcttc tatagtctaa agctgaatgt accacaacta tagcatcatt ttttgccata
    10681 ttaatttcat caaatacccc aaaacctcca ttaatagcac actcataaac tgaacctctt
    10741 ctaagtttaa cttcattgtc tataaacgta tctgtaccta taagtgttga actgtctgta
    10801 tttatatgaa atgatgtatt ccactgaggt ctattaaata tctcacccaa attatcagca
    10861 agtaagtttt ttcctgttgc ctttggtcct gataataata tattttcttc ttctaaaata
    10921 gctgctatac acatagataa tatatctttt ccataaaaat aagttttaga ctttgttact
    10981 ctatattcta aatctttttc taatccataa taatttctaa aatacatcac atcttcaata
    11041 agcttttcat ctaccccttg tgcccttaga ttatctatta tatccattat tcttacccct
    11101 tttatataaa tagtatttgt ttcatttctt atcaccattt tagcataaaa ggatataagc
    11161 tagaattgat tttttaaact aacccacctt aaacaagaca atttatcatt ttagcactac
    11221 aaaaattaat aaggacgaca tgattaagaa ggattcaata acaaatctac ctttacataa
    11281 gaatattaca ctactgtaca atgaaatcag caagtacaag ataaattttg tagatgtgac
    11341 aatcacatta tatatgttta tacgttctta cgattaacca gaataaagca tattacttac
    11401 ttatatttct tgattttatt cgtatacaca tatataatag tagaaaaaat tatgggaagt
    11461 atcataagtt tgttgatttg tgtctgcctt tctgcattta tactgaaagg gctaaaatac
    11521 aatgaaaaaa attaaaatag gtatcttaaa atgaaaaaga taaaaaagct gattatacta
    11581 attgtatttt tgtttctttt tggtatgcta cactgttata ctgaatggaa attcaatact
    11641 gacccaccta aagggataat agaaagaata aatgagtgga ctgataagat tggactggac
    11701 tagtaactta agttaattat agtgaatgta atattagcaa gttattgttt catattttaa
    11761 tactataact ttaaatattt ggttttaatc tgccaaacga atgtaaaaaa actgtcacag
    11821 aggcaacata tttttgaata tttataaagc ggtagttttg attttcaaag ctgccgcatt
    11881 ttcttttata taagcaaact taccaccaac aaattgtaaa acatggtata ttttaaaaaa
    11941 aagcaccaca gacataagcg ataaaagaaa cgccgagaac ctacaaaaac gtagtgttta
    12001 tgcagattta cgacgccttc tattttctca accgtcttaa tgaccttgta caaaagaaat
    12061 ttttctaata ttttttataa tcttatcttg tgatgttttt tatgtataaa tgacttaata
    12121 acaaatccaa taaaaattat ggatattggt ataattagaa ctaggaattt ataatcatta
    12181 taatgtgaca ttattcttac ccaactctca gaaaaaatat atcctatact gattaatgaa
    12241 gtattccata tggttattcc cacgatagat gatactacaa aagttataat attttgacct
    12301 gctatacctg ctataaaaga tatgtatgtt ctacataaag ggattaatct acatacacaa
    12361 acagaaatag aagagtattt attaaaataa tcttgagatg caaatattcc tttctttgcc
    12421 tttggacata tctctattat tttatttatg atagatttac ctccccaatt tcccactgta
    12481 taacaaaata tcgaaccaag tatacctgca attatactta aaattaaaat tgtcaaaaat
    12541 ccaaaatgat ttcttgcagc aattgctcca cataaaggta aaacaacttc gctaggtaat
    12601 ggaaaacaag catactcaat cataattaat acaaatacag agattaatcc atattgtaaa
    12661 ataaaactat taattacaga cacttactta tctatctcct ttcttattta aaggttttaa
    12721 gggtacatat gctaaggatt ctcaccctat atttaaagaa tcaatctctt taaactatta
    12781 tatgtattta cacaaagcat ttataacata ctttaaaaat agtaccttaa aataagaaac
    12841 ttaataaa
//
LOCUS       DBGJFF010000111        12747 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.112, whole genome shotgun sequence.
ACCESSION   DBGJFF010000111 DBGJFF010000000
VERSION     DBGJFF010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12747
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.112"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            105..506
                     /locus_tag="ACOIAF_002960"
     CDS             105..506
                     /locus_tag="ACOIAF_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669961.1"
                     /translation="MKKCLYCNRDIKSPNNKLAIKYDDNMNIYPCRAECKEKIEEYIA
                     KKPTYKFINILEVLLGVGGIFCFLSKWTIAAQVVLGIDALVIFLFPLAGFKMNGKLSM
                     EQTKNQSRSIAISILAFIVIITVLYFKQVGK"
     gene            549..842
                     /locus_tag="ACOIAF_002961"
     CDS             549..842
                     /locus_tag="ACOIAF_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HGZ2669962.1"
                     /translation="MEERKLAPNNEREALIKRLKRIEGQVKGIQKMVEQERYCVDILV
                     QISAIRSAINRVGTIILENHIKGCVAESIKHDDADKTEEMIKELMDTINKFTK"
     gene            857..1297
                     /locus_tag="ACOIAF_002962"
     CDS             857..1297
                     /locus_tag="ACOIAF_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HGZ2669963.1"
                     /translation="MNQQGFIMEIVDDRTAKLKMQRHSACAACGKCATTNSAESKEIL
                     VEVDNTIGAKVGDHVEVSMDNMNVLKAAVMAYIIPLIALLVGTIGTYYILGAIGMTTG
                     VEAISGLVGICLTIICYLYLKKNDKKFRDSREFIPVITRILIDL"
     gene            complement(1389..1862)
                     /locus_tag="ACOIAF_002963"
     CDS             complement(1389..1862)
                     /locus_tag="ACOIAF_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HGZ2669964.1"
                     /translation="MTDMSMILHFLFSGVATAGFAVFFNAPLYLLLPAGVTGGIGWIV
                     YVYLFNFTTNAVFAGFIAAALVSACSETLARKLKQPAIVFVIPGILPLIPGIGLYNTM
                     LSLIQKNYSLAMSKGTDALFLSAAIALGVLVVTSFVRTLNLLKIRKNFIPYRKSK"
     gene            complement(1862..2650)
                     /locus_tag="ACOIAF_002964"
     CDS             complement(1862..2650)
                     /locus_tag="ACOIAF_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HGZ2669965.1"
                     /translation="MNQNEELEYKKNILKLALFVGELMIKNGAETSRVEDSVLRICRS
                     RGFYHVNIFTTPTVIIISDEKFDGFSFMKTIQSRGINLNKISLLNSFSREFVSDKEYD
                     IDNAIARLYEIQDVKPYPLWLFYSCTGIASACFACLLGGNYVLNFILTFIIAIFAAIT
                     YDKTMKISAISAFSCLVSSFLIALSGVLLVEIGILDDPKMLIVGAIMPLLPGVAFIKA
                     IRDLISGNLISGISRAFDAAMIIIAIASGVGFVLDTWYRIGGPF"
     gene            2978..4222
                     /gene="glyA"
                     /locus_tag="ACOIAF_002965"
     CDS             2978..4222
                     /gene="glyA"
                     /locus_tag="ACOIAF_002965"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HGZ2669966.1"
                     /translation="MFENLKKADPEIYESMKRELKRQQRNIELIASENIVSVPVMETM
                     GSHLTNKYAEGYSGKRYYGGCEYIDEVETLAIDRIKKIFGAEHANVQPHSGANANIGV
                     YFAMLKPGDVVMGMNLSQGGHLTHGAPVNISGQYYKFYEYGIDKDSGLIDFDEVRKIA
                     HEVKPKMIVAGASAYPREIDFKKFREIADEVGALLMVDMAHIAGLVAAGLHQNPCEVA
                     DFVTTTTHKTLRGPRGGVILCKEKYAKDIDKAIFPGIQGGPLEHIIASKAVCFKEALS
                     DEFKEYQVQVAKNAKALAEELIKRDFKLISGGTDNHLILLDLTNKNITGKAAEKRLDD
                     AYITANKNTIPFDPNGALVTSGIRLGTPAVTTRGMKEEDMAIIAEAIDLCLTYDEESK
                     ARTLVVGLTEKYPLYEEYNIMD"
     gene            4545..5642
                     /locus_tag="ACOIAF_002966"
     CDS             4545..5642
                     /locus_tag="ACOIAF_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HGZ2669967.1"
                     /translation="MKVLILTGKFGMGHYSASNSLSEDIKAKFDNSEIIIKDIFEYIM
                     PNYSDKMYKTFSILVNRGSSLYNLFYKCAENGKKDIKFTFSDYFLNKLDTLLHEVQPT
                     VVISTFPFCSQLVSRYKEKYNSNLPLITCITDISSHSEWISKNTDCYLVASKSTKEEL
                     VFKGIDESKIKVNGIPVKKEFKRIEHVNHSTKKNILIMGGGLGLLPKSEQFYKELNSL
                     EGVKTTVITGNNKKMYYKLYGRYENIEVVGYTNEVYKYMKDSDLIISKPGGITLFETI
                     YSELPILAFNPFLQQEIDNASFILNNEIGRILGKNKKYYVDEIKDLIYDDATLKEMSS
                     NMKELKKQFDNNTLENILFSLDEQGACRECM"
     gene            5633..6331
                     /locus_tag="ACOIAF_002967"
     CDS             5633..6331
                     /locus_tag="ACOIAF_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HGZ2669968.1"
                     /translation="MYVVGLIIVVALIFLVHSIIPTYYNKLLNKEVLKNMAGENEIAL
                     TFDDGPDKRYTEKLLDVLKENDIQAMFFVVAKNAEKEPEIINRMLRENHIVGLHSLEH
                     RNAWLYSYSYVKKDFIESTNIMKNLGVDVNYYRPPWGHTNIFSNSFVKKYNLKMTLWD
                     VMAEDWEKDSTVDIIINKLMSRTKENSIICLHDAGENSGGAVGAPERTIEALKIAIPK
                     LKASGLKFVTPERM"
     gene            6334..7398
                     /locus_tag="ACOIAF_002968"
     CDS             6334..7398
                     /locus_tag="ACOIAF_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HGZ2669969.1"
                     /translation="MISKTEKKNKCLGSVAFLVLLMGITGYFVFRGQSVESLIKSLKG
                     ASPMFILIGFAMMFIYVACEGINIYLGMKALNQKTTLLKCMGYAFIGFYFSSITPSAS
                     GGQPAQVYYMKKDDINISYSSLILLVIVVIHQVVILAYSGIMFIMEREFILNNVSGMN
                     ILLIYGVITNVALVIGVIAIIFSKKLVNNFIISITNLLGKLRIIKDVESSRKVIICQI
                     EEYVKGAQYIKQNPKLVVQILVITIVQITAMFLVPFFVYKAFHLSTYTVFEILAIQSL
                     LNIAVSSLPLPGAVGASENSFMTLFKIFFPGHLLVPAMLLSRGISFYAFVAISGLICI
                     VVHVKSSEKVKAVKKVAYVR"
     gene            7581..8285
                     /locus_tag="ACOIAF_002969"
     CDS             7581..8285
                     /locus_tag="ACOIAF_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGZ2669970.1"
                     /translation="MNKEIPKKILVADDNPEIREIVEILLTGEGYEVIMATNGEEAVN
                     LVDSTIDLIILDVVMPVKTGFVACNEIREKTTAPILFLTAKTQDSDKVIGFSAGGDDY
                     LSKPFSYSELISRVKSLLRRYYIYQGKEKEREPNILKTKELCVNLDTQDVTLNGEPVF
                     LTTIEYSILILMLKNRKKVFSSENLYETIWDEQYFYTANNTIMVHIRNLRKKLEKDQK
                     NPQYIKTAWGKGYYID"
     gene            8278..9600
                     /locus_tag="ACOIAF_002970"
     CDS             8278..9600
                     /locus_tag="ACOIAF_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HGZ2669971.1"
                     /translation="MINKIKPSKLGVILLIYVVISAAVAFILNIPFYSAVESIVKDVS
                     EGKVYFKREVNQLMDNFQTYVNNNGIRADDGDDIAKWNSDNWFVSMKIYKGNKVFYDT
                     LYYPNEVPDKSEENEFYSSPMARNYEIQFSDIKANIVVVALFRFRFQNFIEAISITFM
                     ALTFIITFLLLFSRKIKYLRKIEMGIKIVESGSLKYRIPVKGNDELSSLASSINEMSH
                     TLENQLIKEREMKNKNKDIVTSISHDVRTPLTSVICYLDLIKDHKYNSPEQLEQYIKN
                     VRSKAYQIKDLTDDLFTHFVNSGEEVSKGYEKINANEFIFQLLLDSSYTLEEKGFHIT
                     IENELYNQYHIYVDVTQFRRIFDNLCSNIIKYARKDKPIVFLIKVEDSKLLIIQKNRV
                     SNFSKNVESFGIGIKNCEKIVAYHNGSINVNSDDKEFAIEISIPAKES"
     gene            9787..11160
                     /locus_tag="ACOIAF_002971"
     CDS             9787..11160
                     /locus_tag="ACOIAF_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HGZ2669972.1"
                     /translation="MVLTGAHYIYILFMVIILITMIMKKDTIVPCILGVFFMGLFFEK
                     NIFGAITAVFNSFIISLNELGPIILIIAIMVALSKALEANNAIQYMVRPFSRVIKNSN
                     TAFFVTGFVMLVLSWFFWPTPAVALVGAVFLPVAMRAGLPAIGVAVALNLFGHGLALS
                     TDFVIQGAPSITAGAAGVAVSYVINDGMILFWVMGIVTISMAFYTLKRDINKGMFREE
                     LKGFESEEVEEFNGKSKIATILVALGFLADIIAMYVFDLKGGDASALLGGTAVFLIII
                     INVINFGKDSLENVCENIIDGFVFGIKIFGAIIPIAAFFYMGEVAPLTGVFGKVLAPG
                     SQGLLSDIGIALSQTIPLNKFAVSGIETVVGAITGLDGSGFSGISLVGSLASVFGTAI
                     NASVGALAALGQISGIWVGGGCLVPWGLISAAAICGVSPIELAKRNFVPVITGLVVTT
                     IVAMFII"
     gene            complement(11532..12470)
                     /gene="pdaA"
                     /locus_tag="ACOIAF_002972"
     CDS             complement(11532..12470)
                     /gene="pdaA"
                     /locus_tag="ACOIAF_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HGZ2669973.1"
                     /translation="MFRKVLHYLTLLSISIFFIVGCSNSQNNQNENQNKETQLQEDKE
                     KIDSGKDTSNVIVSDGTDKPSKATTNNDNNKLDVSSLDNTTLDWFYIPNNKHKTPEVN
                     TDIEFKFSDYDALYNGPTKDGQKTLYLTFDEGYENGYTTKILDTLKQNQVKAVFFVTA
                     PYIKENKDLVKRMVSEGHIVGNHSKTHPSMPTKTSNLKNFNDELYDVEKLYKDVTGKD
                     MVKFFRPPMGKYSEKSLAMTKNLGYKTVFWSFAYRDWDTDKQPSHEEATQKIMDNLHD
                     GSILLLHAVSKTSTEILNDFISNARKLGYEFELLEY"
BASE COUNT         4780 a         1485 c         2144 g         4338 t
ORIGIN      
        1 ttattccttt tatttttgtt ttttgtatct atttggtata atagtattag agcgtataat
       61 aatttaggca aaatttttta aggaagacta gaggagaata taaaatgaaa aaatgtcttt
      121 actgtaatag ggatataaag tcaccaaata ataaattagc tataaaatat gatgacaata
      181 tgaatatata tccatgtcgt gcagaatgta aggaaaaaat tgaagaatat atagcaaaaa
      241 aacctacata taagtttata aatatattag aggttctttt aggtgtgggc ggaatattct
      301 gctttttgag taaatggact attgcagccc aagttgtact tggaattgat gcattggtta
      361 tatttttatt cccattggca ggatttaaga tgaatggaaa gttaagtatg gaacaaacta
      421 agaatcaaag taggtctatt gcaatatcaa ttttggcatt tatagtaata ataactgtat
      481 tatattttaa acaagttggt aagtagagca taattgtata agacatcatt agagtatgag
      541 gtgagtttat ggaagaaaga aaattagcac caaacaatga gagggaagct ctcataaaaa
      601 gattaaaaag aatagaaggt caagttaagg gtatacaaaa gatggtggaa caagaacgtt
      661 attgtgttga tatattggtt caaatatctg ctataaggtc tgctataaat agagtaggca
      721 caattatctt agagaatcat ataaaaggat gtgtagcaga aagcattaaa catgatgatg
      781 ctgataaaac tgaagagatg ataaaagagc ttatggatac tataaataaa ttcacaaaat
      841 aaggaggcgg ttagttatga atcaacaagg atttattatg gaaattgttg atgatagaac
      901 tgcaaagtta aaaatgcaaa gacattcagc ttgtgctgct tgtggaaaat gtgctactac
      961 aaattcagca gagtcaaaag aaatattagt agaagtagac aacacaattg gtgcaaaagt
     1021 tggtgaccat gtagaagtga gtatggataa tatgaatgta ttaaaagcag ctgtaatggc
     1081 ttatataata cctcttatag cacttcttgt aggaacaata gggacttatt atatattggg
     1141 ggcaattgga atgacaactg gagttgaagc cataagtgga ttagttggaa tttgtcttac
     1201 aataatctgt tatttatact taaagaagaa tgacaaaaaa tttagagaca gtagagaatt
     1261 tataccagta ataacaagaa tattaattga cctttaagat aattataatt aaaaaatagc
     1321 aatgtgcttt ttaaataaat actcaatacg tatttgtaga gtttaagtac attgctattt
     1381 ttatattttt attttgattt tctgtaagga ataaagtttt ttctgatttt caaaagattc
     1441 aatgttctta caaaagaagt tacaactaat acaccaagtg ctattgcagc acttagaaat
     1501 agcgcatctg taccttttga catagccaaa gaataatttt tctgaatcaa agacaacatt
     1561 gtattatata gcccaattcc tggtattaga ggaagaatac ctggaattac gaacacaata
     1621 gctggttgct ttagttttct tgctaaagtc tcactgcacg ctgagaccaa agcagcagcg
     1681 ataaaacctg caaaaactgc atttgtagta aaattaaata aataaacata tacaatccaa
     1741 ccaattccgc cagttactcc agctggaaga agtagatata gtggagcatt aaagaaaaca
     1801 gcaaatccag cagtcgcaac acctgaaaat aaaaaatgca aaatcataga catatcagtc
     1861 attagaaagg acctcctatt ctataccatg tatcaaggac aaaaccaact ccagaagcaa
     1921 ttgcaataat aatcatagca gcatcaaaag cacgagaaat tcctgatatt aaattccctg
     1981 atattaaatc tctaatagct tttataaaag caactcctgg gagtagtggc attatagcgc
     2041 cgactataag cattttaggg tcatctaaaa tgcctatttc aactagtaga actcctgaaa
     2101 gtgcaattaa aaaagaagat actaaacagg aaaatgcaga tattgcactt atcttcatcg
     2161 ttttatcata agtaattgca gcaaagattg ctattataaa cgtaagtata aaatttaata
     2221 cataattccc tccaagcaaa caagcaaaac aagcagatgc aattcctgta caagagtaaa
     2281 ataaccataa aggataaggt tttacatctt ggatttcata aagacgtgca atggcattat
     2341 caatatcata ctctttgtca ctaacaaatt ctcgagaaaa actgtttaat aaagaaatct
     2401 tatttaagtt tatacctctt gattgaatcg ttttcataaa cgaaaagcca tcaaatttct
     2461 catctgaaat aattattact gtaggagttg taaaaatatt tacatgataa aaccctcttg
     2521 acctgcatat tctaaggaca ctgtcttcaa ccctagatgt ttcagctcca ttttttatca
     2581 tcagttcccc tacaaataga gctagtttta gaatattttt cttatattca agttcctcat
     2641 tttgattcat aaatacacct ccaaattaaa aaacgtaata ttgaaattat atcaaatttt
     2701 taagtaatat tccttaatat ctatataaat atattatctt ttttttgttt caaaatagtc
     2761 acatttaaat tatatccaat taaaagaatt agacaatatt atttattata ttttaactta
     2821 aaaataaaga aagtaactat gttaaatatg gaaaaatggc aaaacacttg actattaatt
     2881 ggtaaaggac tataataata aattatcata ttgataaaaa cgaaaattat gaaatgtctg
     2941 taaaaataaa atttaaataa taactaggag gagtaatatg tttgagaact tgaaaaaagc
     3001 ggaccctgag atttatgaaa gtatgaaaag agagttaaaa agacaacaaa gaaatattga
     3061 attaatagca tcagaaaaca tagtatcagt tccagtaatg gaaactatgg gaagtcatct
     3121 tacaaataag tatgcagaag gttactcagg caaaagatat tatggtggat gtgaatacat
     3181 agatgaagtt gaaactcttg caatagatag aatcaaaaaa atatttggag cagaacatgc
     3241 taatgttcaa cctcactctg gagcaaatgc aaatattggt gtatattttg ctatgttaaa
     3301 accaggtgat gttgttatgg gaatgaattt atctcaaggt ggacatttaa ctcatggtgc
     3361 tccagttaat atttctggtc aatattataa attttatgaa tatggtatag ataaagacag
     3421 tggacttata gattttgatg aagttagaaa gatagcacat gaagtaaaac ctaaaatgat
     3481 agttgcagga gcaagtgcat atcctagaga aatagatttt aaaaagttta gagagatagc
     3541 tgatgaggtt ggagcattgc ttatggtaga tatggctcat atagcaggtc ttgtggctgc
     3601 tggtcttcac caaaatccat gtgaagtggc tgattttgtt acaacaacaa ctcacaaaac
     3661 tttaagagga cctcgtgggg gtgttattct ttgtaaagaa aaatatgcta aagacataga
     3721 caaagcaata ttccctggta ttcaaggtgg acctcttgaa catataattg cttctaaggc
     3781 agtttgcttt aaagaagctt taagtgatga gtttaaagaa tatcaggtac aagttgctaa
     3841 aaatgcaaaa gccttagctg aagaacttat taaaagagat tttaaattga tttctggagg
     3901 tacagataat catttaatac tattagattt gactaataaa aatataactg gtaaagcagc
     3961 agaaaagaga ttggatgatg cttatataac agctaataaa aatacaattc catttgaccc
     4021 aaatggtgct cttgttacaa gtggaataag acttggtact ccagctgtaa ctactagagg
     4081 tatgaaagaa gaagatatgg caataatcgc tgaggctata gacctatgtt taacttatga
     4141 tgaagaatct aaggctagaa cattggttgt tggtttaact gaaaaatatc ctttatatga
     4201 agaatataat attatggatt aattataaag aacaactaaa taaaatacat ttaaataatt
     4261 acaatgattg tgtatgtatt tgaatatatt tagatataaa aaaggtgtct caaaatgaac
     4321 tttttagttt atgaggcact tttttgtatg gaatcgaaca tatttgtatt atttaaataa
     4381 taaaaaatat ggttatctct atttttagat ggtctctttg tattttttaa ttaaaacttt
     4441 aagatttctt aagaattata ctaggttttt ctaaagattt aaagtctata cttataagta
     4501 tagtaatcat atgaaaagaa aagctttaac gaggagggaa tcttatgaag gtattaattc
     4561 ttacaggaaa atttggtatg ggtcattact cagcatcaaa ttctttaagt gaagatataa
     4621 aagcaaaatt tgataattca gaaataataa ttaaagatat atttgagtac attatgccta
     4681 actattcaga taaaatgtac aagacttttt ctattcttgt aaatcgggga agtagtcttt
     4741 ataatttgtt ttataaatgt gctgaaaatg gaaagaaaga tataaaattt actttttcag
     4801 attatttttt aaataaatta gatacattac ttcatgaagt acaaccaaca gtagttattt
     4861 caacttttcc attttgttct caattagtat caagatataa ggaaaaatat aactcaaatc
     4921 ttcctttaat aacatgcata actgatataa gtagtcactc agaatggatt agtaaaaata
     4981 cagactgtta tttagtagct agtaaatcta ctaaggaaga acttgttttt aaaggaatag
     5041 atgaatctaa gattaaagtt aatggtattc cagtaaaaaa agaatttaaa agaatagaac
     5101 atgtaaatca ttcaactaaa aaaaatattc ttataatggg tggaggatta ggattactgc
     5161 ctaaatcaga acaattttac aaagaattaa atagtttaga aggagtaaaa actactgtaa
     5221 taactggtaa taataaaaaa atgtactata aactatatgg tagatatgaa aatatagagg
     5281 tagtaggtta caccaatgaa gtatataaat atatgaaaga ttctgacctt attatttcta
     5341 aaccaggtgg aataacacta tttgaaacaa tttattcaga acttccaata ttggcattta
     5401 atccattttt acaacaagag atagataatg ctagttttat attaaataat gaaattggga
     5461 gaatacttgg taaaaataaa aaatactatg tagatgagat aaaagattta atttatgatg
     5521 atgctactct taaagaaatg agcagtaaca tgaaagaatt aaaaaaacaa tttgataata
     5581 ataccttaga gaatatttta ttttcacttg atgaacaagg agcgtgtaga gaatgtatgt
     5641 agtaggttta attatcgttg ttgcattgat ttttttagtc cattcaatta tacctacata
     5701 ctataataaa ctattaaata aagaggtatt aaaaaatatg gctggagaaa atgaaattgc
     5761 acttacattt gatgatggtc cagataagag atatacagaa aaattactag atgtgttaaa
     5821 agaaaatgat atacaagcta tgttttttgt agtagcaaag aatgctgaaa aagagcctga
     5881 aattataaac agaatgttga gagaaaatca tatagttggt ttacattcat tggaacacag
     5941 aaatgcatgg ctttatagtt atagttatgt taaaaaagat tttattgaaa gtacaaatat
     6001 aatgaaaaat ttaggtgtag atgtaaacta ctatcgtcca ccatggggtc atactaatat
     6061 attttcaaat tcatttgtta aaaaatataa tctaaaaatg actttgtggg atgtaatggc
     6121 agaagattgg gagaaagata gtacagtaga cattattata aacaagttaa tgagtagaac
     6181 aaaagaaaat tctataattt gtttacatga tgcaggtgaa aattcaggtg gtgcagtagg
     6241 tgcacctgag cgtactattg aagctttaaa gatagctatt ccaaaactta aagccagtgg
     6301 tttaaagttc gtaacaccag aaaggatgta gttatgataa gtaaaactga aaagaaaaat
     6361 aaatgcctag gtagtgtagc ttttttagtt ttactaatgg gcataacagg atattttgtt
     6421 tttagagggc agtcagttga atcattgata aaatcattaa aaggtgcaag ccctatgttt
     6481 atactaattg gatttgcaat gatgtttata tatgttgcct gtgaaggtat aaatatatac
     6541 ttaggtatga aagctttaaa ccaaaagact actcttttaa aatgtatggg atatgctttt
     6601 attggatttt attttagttc tattacgcct tcagcatcag gtggtcaacc agcacaagtt
     6661 tattatatga aaaaagacga tataaatata tcatattctt cacttatact attagtcatt
     6721 gttgttattc accaagtagt tatactggca tatagtggaa taatgttcat tatggaacgt
     6781 gagtttatat taaataatgt aagtggtatg aacattttac taatatatgg tgtaataaca
     6841 aatgttgctc ttgtcattgg agtaattgca attatatttt caaaaaaact tgtaaacaat
     6901 tttataatat caataacaaa tttattaggt aagttaagaa taataaaaga tgttgaaagt
     6961 tcaagaaaag ttataatatg tcaaattgaa gagtatgtta aaggtgctca atatataaag
     7021 cagaatccaa aacttgtagt ccaaatttta gtcataacta ttgtacaaat aacagctatg
     7081 tttttagttc ctttctttgt atacaaagct tttcatctat caacatatac tgtatttgag
     7141 atattagcga tacaatcctt gcttaatatt gctgtttctt cattaccatt gccaggggct
     7201 gttggagctt ctgaaaatag ttttatgaca ttatttaaga tatttttccc tggtcactta
     7261 ttagttccgg caatgttact tagtagggga ataagttttt atgcatttgt agctataagt
     7321 ggattgatat gtatagtagt acatgtaaaa tcttctgaaa aagtaaaagc agtgaaaaaa
     7381 gttgcttatg taagataata ttatatagat tatttttatt aatatgtcaa tttgtgtgta
     7441 tattttaatt aagctttaaa catgtttaca ggagtagaaa ctattaaaac gtgtgacatt
     7501 gctaagcttt tgctaataaa gtataatatt ttatatagtt tgaaatattt aagaaatata
     7561 gcaaaaaaag gagcttggtg atgaataaag agattccaaa aaaaatttta gtagctgatg
     7621 ataatcctga gattagggaa atcgtagaaa tacttttaac tggtgaaggt tatgaagtta
     7681 ttatggcaac gaatggagaa gaagctgtaa acttagtaga tagtacgatt gatttgatta
     7741 ttttggatgt tgtaatgcct gtaaaaacag gatttgttgc atgtaatgaa attagagaaa
     7801 aaacgacagc accaatatta tttttaactg caaaaactca agactctgat aaagttattg
     7861 gattttcagc aggaggagat gactatttat caaagccttt ttcatattct gaactaattt
     7921 caagggttaa gtctctactt agacgttatt atatatacca gggaaaggaa aaggaaagag
     7981 agccaaatat attaaaaaca aaagagttat gtgtcaattt agatactcaa gatgttacac
     8041 taaatggaga acctgtgttt ttgacaacta tagaatatag tatattaatt cttatgttga
     8101 aaaatagaaa aaaagttttt tcgtctgaaa atttatatga aactatttgg gatgagcagt
     8161 atttttatac agcaaataat acaataatgg ttcatattag aaatttaaga aaaaagttag
     8221 aaaaagacca aaaaaatcca caatatataa aaaccgcatg gggaaagggg tattatattg
     8281 attaacaaaa ttaagccatc taagttggga gttatactat tgatatatgt agtaatctca
     8341 gctgcagtgg ctttcatttt aaatatacca ttttactctg ctgttgaatc tatagtaaaa
     8401 gatgtatcag aagggaaagt atattttaaa agagaagtaa atcaacttat ggataatttt
     8461 caaacttatg tgaataacaa tggtataaga gcagatgatg gagatgatat agcaaaatgg
     8521 aatagtgata attggtttgt ttctatgaag atatataaag gaaataaggt tttttatgat
     8581 acattatact atcctaatga agtacctgat aagagtgagg aaaatgagtt ttatagcagt
     8641 cccatggcta gaaactatga gatacaattt tctgatatta aagctaatat agttgtagta
     8701 gctttattta gatttagatt tcaaaatttt atagaagcaa tttctattac atttatggca
     8761 cttacattta ttataacatt tttactgttg tttagtagaa agataaagta cctaaggaaa
     8821 atagaaatgg gtataaagat agtagagagt ggcagtttaa agtatagaat cccagtaaaa
     8881 ggtaatgatg aattatcatc acttgcatct agtattaatg agatgagtca tacattggag
     8941 aatcaactta taaaagaaag ggagatgaag aataaaaata aagatatagt aacttcgata
     9001 tcacatgatg ttagaactcc attaacgtca gtaatttgtt atttagattt gattaaagac
     9061 cataaataca atagtcctga gcaattggaa caatacataa aaaatgtaag gtctaaagca
     9121 tatcaaataa aagatttaac agatgacctt tttacgcatt ttgttaattc tggagaggaa
     9181 gtttcaaaag ggtatgaaaa aataaatgct aatgaattta tttttcaatt acttttagat
     9241 agttcatata cattggagga gaaaggattt catataacaa tagaaaatga actttataac
     9301 caatatcata tttatgtaga tgttacacaa tttagaagga tatttgataa tttatgttct
     9361 aatataataa aatatgctag gaaagataag cctatagtat ttttaattaa agtagaggat
     9421 tctaaattac taattataca aaaaaataga gtaagtaatt tttctaagaa tgtagaaagt
     9481 tttggaattg gtataaagaa ttgtgagaaa atagttgcat atcataatgg aagcataaat
     9541 gtaaattcag atgataaaga atttgctatt gaaatatcta ttccagcaaa agaatcataa
     9601 aaatttaaat tatatattgt gtagttgcta ttatatattg tgtaattgct tatgtaaaat
     9661 tctatgaagc aggaagcatg tgactttagt tatgggatgt tcaatattgt gtacctctag
     9721 gcaagtaaca agtttactta caagaccata taatattatt atcttaaaaa aaggggacat
     9781 aataatatgg ttttgacagg agcacattat atttacattc tatttatggt aattatatta
     9841 attactatga ttatgaaaaa ggatacaata gtaccatgta tattaggtgt tttttttatg
     9901 ggattgttct ttgaaaaaaa catttttggt gctataacag cagtgtttaa ctcttttatt
     9961 atatctttaa atgagttggg tccaattatc ttgattatag ctattatggt tgcattatct
    10021 aaagccctag aagccaataa tgctatacag tatatggtga gaccattttc gagagttata
    10081 aaaaattcta atacagcatt ttttgtaact ggatttgtta tgttagttct ttcttggttt
    10141 ttctggccaa ctccagctgt tgccttagtt ggagctgtat ttttacctgt tgctatgaga
    10201 gctgggctac cagcaattgg tgttgcagta gcattaaatt tatttggtca tggtcttgcc
    10261 ttgtctacag actttgttat tcaaggagct ccaagtataa cagctggtgc agcaggtgtt
    10321 gcagtatcat atgttataaa tgatggtatg attttgtttt gggtgatggg aatcgtaacc
    10381 atatctatgg cattttatac attaaaaaga gatattaaca aaggaatgtt tagagaagaa
    10441 ctaaaaggtt ttgaaagtga agaagtagaa gagttcaatg gaaaatctaa aatagcaact
    10501 attttagtag ctctgggatt tcttgctgat ataattgcta tgtatgtatt tgacctaaaa
    10561 ggaggagatg catcagctct tttgggtgga acagcagtat ttttaataat aattataaat
    10621 gttataaatt ttggcaaaga tagtctcgaa aatgtatgtg agaatataat tgatggtttt
    10681 gtgtttggga taaaaatatt tggagcaatt ataccaatag cagcattttt ctatatgggt
    10741 gaagttgcac cattgacagg tgtatttgga aaagtattag caccaggttc acaaggttta
    10801 ttatcagata taggcatagc tctttctcaa acaataccac ttaataaatt tgccgtatca
    10861 ggtatagaaa ctgtagtggg agcaataact gggcttgatg gttctggatt ttctgggatt
    10921 tcattagttg gttcacttgc ctctgtattt ggaactgcta taaatgcaag tgttggtgca
    10981 ttagctgcat taggacaaat cagtggtata tgggtaggtg gaggctgtct agttccttgg
    11041 ggtcttattt cagcagcggc aatttgtggg gttagtccaa tagaattggc aaagcgaaac
    11101 tttgtcccag ttataaccgg attggttgta acaaccatag tagcaatgtt tattatttaa
    11161 taaatcgaat aaatctttaa ttgtataaat taaaaataga tgtgtagcta taatttatta
    11221 taattgtttt actaattcta agccatacat ctatttttat tgatattttt attagtattc
    11281 tttaataaag taatattttt cattatacta tacataacat ctaaatataa catctaaaat
    11341 actgttatct atatttaata tcttgccaaa ttttttgatg ttttattgcc ctgtcccacc
    11401 aacatatata agctatcaca agtttttacc tatactatca ccaaacaaaa attctaattt
    11461 taatttatta ttttataacc tatattatat taactatatt agtttgtaga ctttagatgt
    11521 aattataaat attaatactc caagagttca aattcatatc ctagttttct tgcatttgat
    11581 ataaaatcat ttaaaatttc tgtactagtt ttggaaactg catgtagtaa caagatagaa
    11641 ccatcatgta gattatccat tattttttgc gttgcttctt catgtgatgg ttgtttatca
    11701 gtatcccagt ctctgtatgc aaaactccaa aatacagtct tgtatcctaa atttttagtc
    11761 attgccaagc ttttttctga gtattttccc ataggaggtc taaaaaattt aaccatatct
    11821 ttcccagtta catctttata taacttttca acatcatata attcgtcatt aaagtttttt
    11881 agattgcttg tttttgttgg catagatgga tgggttttac tatgatttcc aactatatgt
    11941 ccctcagaaa ccattctttt tactaaatct ttattttctt ttatatatgg agctgtaaca
    12001 aaaaaaactg ctttaacctg gttttgtttt agagtgtcga gtatctttgt agtgtatcca
    12061 ttttcataac cttcatcaaa tgtaagatat agtgtttttt gaccatcttt tgtaggtcca
    12121 ttgtataggg catcatagtc agaaaattta aattcaatgt ctgtatttac ttctggtgtt
    12181 ttatgcttat tgtttggtat ataaaaccaa tcaagagtag tgttatctaa agaagataca
    12241 tctaatttat tattatcatt attagtagtg gcttttgatg gtttatctgt tccgtctgaa
    12301 actattacat tggaagtatc tttaccacta tctatttttt ccttatcttc ttgtaactgt
    12361 gtttccttat tttgattttc gttttgattg ttttgtgaat ttgaacatcc aacaataaaa
    12421 aaaatagaaa tagaaagtag tgttaaataa tgcaatactt ttctaaacat tttgaagctc
    12481 ctttctaaat taataataat tgcatataat aataattata tgattaatta caattgcata
    12541 tagtattatt ataattggaa attactatta ataatagtta tatctttaaa aataaagtca
    12601 caatagtatg acaaagggtg aataatcaat aaatttaaaa aaaaatgaaa aaaatagttg
    12661 accatattat gagaagatgt tattataata aatgtgctca acaagaagta acaaaataac
    12721 aagcacaaag aagttaattg aatgaaa
//
LOCUS       DBGJFF010000112        12617 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.114, whole genome shotgun sequence.
ACCESSION   DBGJFF010000112 DBGJFF010000000
VERSION     DBGJFF010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12617
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.114"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            <1..577
                     /locus_tag="ACOIAF_002973"
     CDS             <1..577
                     /locus_tag="ACOIAF_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGZ2669974.1"
                     /translation="NVYHTKEDMIDCVNAFYEGMVQRSEEMKRHPDYKTGENYAYLGL
                     PPCFLIFDEYVAFFEMLGTKESVSLLSHLKKIVMLGRQAGYFLIVACQRPDAKYFSDG
                     IRDNFNFRVGLGRISELGYGMLFGSDVKKQFFQKRIKGRGYCDVGTSVISEFYTPLVP
                     KGHDFLQTIGSLAQARQDGTATCEAQGDGTD"
     gene            779..1978
                     /gene="mobT"
                     /locus_tag="ACOIAF_002974"
     CDS             779..1978
                     /gene="mobT"
                     /locus_tag="ACOIAF_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HGZ2669975.1"
                     /translation="MNETDFLTALKEKRCAYGVSQTRLAIMANISREHLSRIEAGKVT
                     LTEDMKHTLLEAVEKFNLDNPMFLLFDYVRIRFPTMDIKHIIKDILKLNIGYMLHEDY
                     GHYKYTEHYHIGDVFVYVSQDEEKGVLLELKGKGCRQFESYLLAQERSWYDFFTDALV
                     EGGVMKRLDLAINDRTGLLDIPELIRKCENEECISKFRSFKSYGSGELVKHNETDKYG
                     MGHTLYIGSFSSEVYFCCYEKNYEQYAKLGIPIEEVPIKNRFEIRLKNERAYYAVVEL
                     LTHYDAEQTAFSIINQYIRFADKEPDKRKSDWKTNVRWAWFIGEGRPPVKLTTKPEPY
                     TLERTLNWLQRQVAPTLKMLKQIDKDNQTEYLETIEKQAKLTERHHQIIKQQTATVEE
                     MVAKVKE"
     gene            1986..2120
                     /locus_tag="ACOIAF_002975"
     CDS             1986..2120
                     /locus_tag="ACOIAF_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017143882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HGZ2669976.1"
                     /translation="MWELLKDLLLVSLGMGIGVVLMCILTVGKEADRCMEEIKEREEH
                     "
     gene            2862..4772
                     /gene="ltrA"
                     /locus_tag="ACOIAF_002976"
     CDS             2862..4772
                     /gene="ltrA"
                     /locus_tag="ACOIAF_002976"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HGZ2669977.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGTPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMPDGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWHENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKTKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKTQKDKLVSQAKKI
                     ASPSKGKTELEEIRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGASKKG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYSANCYTPEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTRAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            4876..5100
                     /locus_tag="ACOIAF_002977"
     CDS             4876..5100
                     /locus_tag="ACOIAF_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669978.1"
                     /translation="MSGTYLSIYTWFLSNAQSLVLMAIVVIGIYLGFKREFSKLIGFL
                     VVALVAVGLVFNAGGVKDVLLELFNKIIGA"
     gene            5181..5558
                     /locus_tag="ACOIAF_002978"
     CDS             5181..5558
                     /locus_tag="ACOIAF_002978"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGZ2669979.1"
                     /translation="MDYKVDDKNLDASMFISFVNQVWQGDYDMEQTRQALSKTLNITA
                     YDNKKLVGCLRILSDGYYFGTITELLVLPQYQKQGIGSKLLQLAKDNTPTMLYFGSQP
                     EAEKFYEKNGCQKSLQSYMINKG"
     gene            5665..5961
                     /locus_tag="ACOIAF_002979"
     CDS             5665..5961
                     /locus_tag="ACOIAF_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005339688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HGZ2669980.1"
                     /translation="MNDIFKDMQEKVSCENISDLPSYKRKVWHEMKQLNIADYEEKQL
                     EDFSKYVFGMSYQTLKDVIKQQKGREEQCRKQGCWWKRAEQLVKKQRHTGSTCQ"
     gene            5880..6383
                     /locus_tag="ACOIAF_002980"
     CDS             5880..6383
                     /locus_tag="ACOIAF_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HGZ2669981.1"
                     /translation="MQETRVLVETSGTTGEETTSYWFDLPIDVAEFEEKLGVGAESQD
                     YRIIEKVLPYADEVHEHTSVYQLNELDYMYRQLSSDMQEEYVSLLTVYENLEALYICR
                     NVITVYPDCKSMIDVARQKLMSDPTFKHLSEDCQEYYFDFEAYASHLQEHGKFLVTEH
                     GIFELPE"
     gene            6401..6904
                     /locus_tag="ACOIAF_002981"
     CDS             6401..6904
                     /locus_tag="ACOIAF_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HGZ2669982.1"
                     /translation="MIDDMAVYIANLGKYNEGYLVGAWFTFPIDEEDVKEKIGLNEQY
                     EEYAIHDTDNFPIEIGEYVSIEELNEMYEMIKELPDYIVDCLDEFIGHYGTLEEVVEH
                     KDDIYYYPDCETMTDVAYFYIDELQALGDIPPNLQNYIDYEAFGRDLDMGGYFIETSR
                     GMCEIPY"
     gene            7016..7414
                     /locus_tag="ACOIAF_002982"
     CDS             7016..7414
                     /locus_tag="ACOIAF_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HGZ2669983.1"
                     /translation="MKKIKSYTGIWNVEKVLYAINDFNLPFPVTFTQITWFVITEFII
                     ILFGDMPPLSMIEGAFLKYFGIPVALTWFMSQKTFDGKKPYSFLKSQITYALRPKITY
                     AGKAVKLDKQILNETITAVRSVNDVPDKIY"
     gene            7392..9842
                     /locus_tag="ACOIAF_002983"
     CDS             7392..9842
                     /locus_tag="ACOIAF_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGZ2669984.1"
                     /translation="MFPIKYIDNNLVWNKDNEVFAYYELIPYNYSFLSAEQKFIVHDS
                     FRQLIAQSREGKIHALQIATESSIRSMQEQSKKLVTGKLKEVACQKIDEQTEALVSMI
                     GDNQVDYRFFLGFKLMVTEEQLNLKNIKKSAWLTFTEFLHEVNHTLMNDFVSMPNDEI
                     NRYMKMEKLLENKISRRFKVRRLEINDFGYLMEHLYGRDGIAYEDYEYQLPKKKLQKE
                     MLIKYYDLIRLTRCVIEESQRYLRLEHEDKESYVSYFTVNAIVGELDFPSSEIFYFQQ
                     QQFTFPVDTSMNVEIVENRKALTTVRNKKKELKDLDNHAYQAGSETSSNVVDALDSVD
                     ELETDLDQTKESMYKLSYVVRVSADDLDELKRRCDEVKDFYDDLNVKLVRSAGDMLGL
                     HSEFLPASKRYINDYVQYVKSDFLAGLGFGATQQLGENTGIYMGYSVDTGRNVYLQPS
                     LASQGVKGTVTNALASAFVGSLGGGKSFCNNLLVYHSVLFGGQAVILDPKSERGNWKE
                     TLPEIAHEINIVNLTSDKDNAGLLDPFVIMKNVKDAESLAIDILTFLTGISSRDGEKF
                     PVLRKAVRSVTQSETRGLLHVIDELRKEDTAISRNIADHIDSFTDYDFAHLLFSDGMV
                     ENAISLDNQLNIIQVADLVLPDKDTTFEEYTTIELLSVSMLIVISTFALDFIHSDRSI
                     FKIVDLDEAWAFLNVAQGETLSNKLVRAGRAMQAGVYFVTQSAYDVSKESLKNNIGLK
                     FAFRSTDINEIKQTLEFFGIDKDDENNQKRLRDLENGQCLLQDLYGRVGVVQIHPVFE
                     ELLHAFDTRPPIQRNEVE"
     gene            9842..12037
                     /locus_tag="ACOIAF_002984"
     CDS             9842..12037
                     /locus_tag="ACOIAF_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HGZ2669985.1"
                     /translation="MKKRIKGAFTKRKILHFLKMALFVVALSLILLSLLGTVAHATGL
                     VDDTINAENLYSKYPLSNYQLDFYVDNSWSWLPWNWLDGIGKSVQYGLYCITNFVWTI
                     SLYLSNATGYVVQEAYKLDFINDMADSIGQSIQTLAGVTQNGFSSTGFYVGFLLLIIL
                     VVGIYVAYTGLIKRETSKALHAVINFVVVFVLSASFIAYAPDYIKKINEFSSDISTAS
                     LDLGTKIMLPNSDSEGKDSVDLIRDSLFSIQVEQPWLLLQFGNSNAEEIGTDRVEALV
                     SVSPEDEDGKTREEIVKTEIEDNDNNILTIPQVVNRLGMVFFLLFFNLGITIFVFLLT
                     GMMLFSQILFIIFAMFLPISFLLSMIPSYESMAKQAIVRVFNTIMTRAGITLIVTVAF
                     SISSMFYNISTDYPFFMVAFLQIVCFAGIYMKLGDLMSMFSLNASDSQSMGRRIFRRP
                     YLFMRHRARRMERRMARAVSAGGISGGVAGALAGSTVSNARTTRNNPTKENRGNTTSS
                     MGQRAGSKIGAVLDTKNKVKDKANAVKENVKDMPTQTAYAVYSAKEKAKSSVSDFKRG
                     MVQEQQSRQTGRLEKQEQRKKNIANKRMELQKAQEARQARKADGSATTGATRPHERPA
                     TASKPSVEKVQEVKRPATTPTSKASEPVKTNVIKERPLSSGASDGKATQSAQPIHRQN
                     IEKVVSQETRQNYVKDRRTKVQQTQSVQKNQQTTEKNHNLVTKKGQKKK"
     gene            12034..>12617
                     /locus_tag="ACOIAF_002985"
     CDS             12034..>12617
                     /locus_tag="ACOIAF_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HGZ2669986.1"
                     /translation="MKLKHIALIGSLFPILFSIVLFLGVLISADSDDENSNFSSGITG
                     LNLSAEVLKHQPMVEKYAREYGISEYVNVLLAIIQVESGGTAEDVMQSSESLGLPPNS
                     LDTESSIKQGCKYFASLLSSYKNQGINDLNVAIQSYNYGGDYVGYVAGKGKKHTFTLA
                     ESFAREKSGGKKVTYTNPIAVAKNGGWRYGYGNM"
BASE COUNT         4135 a         2346 c         2907 g         3229 t
ORIGIN      
        1 aaatgtgtat cacaccaaag aagatatgat tgattgtgtc aatgcctttt atgagggcat
       61 ggtacaaaga agcgaagaaa tgaagcgaca cccagactat aaaacgggcg aaaactacgc
      121 ctatcttggt ttaccgccct gctttcttat ctttgatgaa tatgtggcgt tttttgaaat
      181 gctggggaca aaagaaagcg tgagcctact tagccattta aagaaaatcg tcatgctggg
      241 gcgacaagct ggatatttcc ttatcgttgc ctgccagcgt ccagacgcaa aatatttctc
      301 agacggtatc agagataatt tcaatttccg tgttggattg gggcgtatca gcgaattggg
      361 ttacggtatg ctgttcggtt cagatgtgaa aaagcagttt ttccagaagc gtatcaaggg
      421 gcgtggctat tgtgatgtgg gaacaagcgt cataagcgaa ttttacacgc ctttagtccc
      481 gaaaggacat gattttttgc agactatcgg ctctcttgca caagcaaggc aggacgggac
      541 ggcgacgtgc gaagcgcaag gcgacggcac ggactagccg ttgctggtgt ggcgttagcc
      601 acgccagcag aaacaagccc ctcattatct aacagagggg cacaattacg gcggaaactt
      661 ccacaaaaac ctttgcaaga ccgcataaaa taagggcttt tcgtacattt cgccttttgt
      721 gataaagcct tgaaattatc acaatatcac aaaattatca cgattggggg tatgacaact
      781 gaacgaaact gattttttaa ccgccttgaa agaaaaacgc tgtgcatacg gcgtatcaca
      841 gacaaggctt gcaatcatgg caaacatcag ccgtgaacat ttgagccgta tcgaagcagg
      901 aaaggtaacg ctgaccgagg acatgaaaca tacgctttta gaagcggtgg aaaaattcaa
      961 tctcgataat cccatgttcc tgctgtttga ttatgtgcgt atccgttttc ccacaatgga
     1021 tataaagcat atcatcaagg atatattaaa gctcaatatc ggctatatgc ttcacgaaga
     1081 ttacggacat tacaaataca ccgagcatta ccatatcgga gatgtgtttg tgtatgtatc
     1141 gcaggacgaa gaaaaaggtg tcctgctgga actgaaaggc aaaggctgtc gccagtttga
     1201 aagctatctg ctggcacaag aacgaagctg gtatgacttc ttcacggacg cattggttga
     1261 gggcggtgtg atgaagcgtc ttgatttggc aatcaatgac cgaacgggtt tacttgatat
     1321 tccagagctg atacgaaaat gcgagaatga ggaatgtatt tctaaattcc gttctttcaa
     1381 aagctatggt tctggggaac tggtaaagca taacgaaacg gataaatacg gtatgggaca
     1441 cacgttgtat atcggttcat tttcaagtga agtgtacttc tgctgttatg aaaagaatta
     1501 cgaacagtac gccaaacttg gcataccgat tgaagaagtc cccataaaaa atagatttga
     1561 gatacgtttg aaaaatgagc gtgcctatta tgctgttgtg gaactgctga cacattatga
     1621 cgcagaacag acagcatttt ctattatcaa ccagtatatc cgcttcgctg ataaagaacc
     1681 agacaagcga aaaagcgact ggaaaacaaa tgtacgctgg gcttggttca ttggcgaggg
     1741 gcgaccaccc gtaaagctga caacaaagcc agaaccgtac acgttagaac gtacactcaa
     1801 ctggctacaa aggcaggttg caccgacctt gaagatgttg aaacagatag acaaggacaa
     1861 tcagacggag tatctggaaa cgattgagaa gcaggcaaaa ctgaccgagc gacaccacca
     1921 gataatcaag caacagacag ccacggttga agaaatggtt gccaaagtaa aggagtgatg
     1981 aaaaaatgtg ggaattattg aaagacttac tgctggtatc tctgggaatg ggtatcggcg
     2041 tagtcttgat gtgcatttta actgttggca aagaagctga cagatgtatg gaagaaatca
     2101 aagaaagaga ggaacattaa atgaattttg gacaaaattt atgtgcgact cgttccttag
     2161 tgaaaagcta aggcactaat aaaagaacga aaaagtttga ttattaggag aactgataat
     2221 ctgacaggtg gaatgatgaa gtaacgctct gaaacacctg ccctgagcct ccgacaagca
     2281 ttgaatgagg aaactcagaa tgtttgaagc tcggtaaaga cggctgaaag ataccgtaat
     2341 agttgatgaa catatatctt ctatcgaaaa ctatccagag gtgggtagcg tatccaacag
     2401 gtcgggggtc tataaaatat ctatggttag aatgttccac aagggactga cgaaacagcg
     2461 aatgtacggg tctaaatttg tactttgtag aaatgcaaag gtacgagaaa tcgtagctgg
     2521 tgtggcaaag taagaatata ggttatgaaa tgccatatac cgttacaggc ggtatcaagc
     2581 cagcaggctt atagctagtg acctaaggat atatatgtat agataagatt atcggaacga
     2641 ggaaaggtat tgggttctca tcaagagaat aagctgacga agaacaataa gcagttgaac
     2701 caatgctgaa aagcagaggg cgaacctaag aaattcttgt aatgagaatg gaggaatacc
     2761 ctcaagtcgt gtatatatta gaaaagtatt atgttgtttt aataaggatt acgagtaaga
     2821 taaaaaagct cactcaattt caagcaaagg agtgaagcct tatgcctaaa aataaaaatg
     2881 aaacattgtg tgtagaagat ttaagacacg ctgaatatta cgaaatgcaa aacacctttg
     2941 atgatttata tgccaaaagt aagaatggag atattttcac tcatcttatg gatattatcc
     3001 tatcgagaga aaatatatta ctggcatatc gaaatatcaa agcaaatgca ggcagtaaga
     3061 cagcaggaac agacggtaca ataattaaag atattggcaa attgccagca gaaacagttg
     3121 tcaaaaaggt aagatatatt gtcgcaggca ctccgcacgg gtatcgacct aagccagtaa
     3181 ggcgaaaaga aataccaaaa ccaaatggga aaaccagacc tctaggcatt ccttgtatgt
     3241 gggacaggct tatacaacaa tgtatcaaac aagtattaga accaatctgc gaagctaaat
     3301 ttagtgaaaa cagctacggt ttcagaccta ataggtcggt tgaaaatgca ataaaagcaa
     3361 cctataaccg cctacaaatc agccagctac attatgtgat tgaatttgat attaagggtt
     3421 tctttgacaa cgtgaaccat tccaaactga taaaacagat atgggctatg ggaatacggg
     3481 acaagcactt gatattcatt ttaaaaagga tattaaaagc tccaattaaa atgcctgacg
     3541 gcacaataac atatccagag aaaggaactc cacagggggg aattatatcc ccactactag
     3601 ccaacattgt tcttaatgag ttagaccatt gggtagaaag tcaatggcat gaaaatcctg
     3661 ttacaaaaaa ctatgtggtt catatcaata aaagcggtag cccatgtaaa agcaacgctt
     3721 acaaagaaat gaagaaaaca aagttaaagg aaatgtatat ggttcgttat gcagatgatt
     3781 ttagagtttt ctgtcgctac aaagaaagtg ctgaaaaaac caaaatagca attactcaat
     3841 ggatcgagca acgactaaaa ttggaagtat cacaagaaaa aacaagaatt gtaaatgtta
     3901 gaaaaagata ctccgacttt cttggtttca agattaaaat gataccgaga agaaagaagt
     3961 tagttgttaa atcacatata agtgataaac aattcaagac ccaaaaagac aagctggtat
     4021 cacaggcaaa gaaaattgct tctccctcaa agggaaaaac agaattagag gaaataagac
     4081 tttataattc aatggtatta ggtatgcaaa attattatca gattgcaaca aatgtaaatc
     4141 tggattgtat gaaacttaat agagctgtaa tggctatctt cactaacaga ttaggtgcaa
     4201 gcaaaaaggg cagattgaga aaaacaggca gacaacttac aacaaaagaa gcaacaagat
     4261 atggtaaatc taaaatgctt cgatatgttg ctggcgaggg cgaacctata ttccctgttg
     4321 gatatatcca acacagaaaa ccaatggcaa aagtgtattc agcaaactgc tatactccag
     4381 agggaagaaa atttatccat accaatctaa gtgttgataa attactgatg tatcagttga
     4441 tgaaattatc tttggaaaat cgcacaatag aatacgcaga caatcgaatt tcgctatttt
     4501 ctgcccaaca tggaaaatgt gctataactc tggaagaatt tcaacaagct aatgaaatac
     4561 actgtcatca tattgtacca actagggctg gtggtaatga cgattataaa aatcttattt
     4621 tggtaaaaga ggcggtacat cgtctaatac acgcaaaaac agaagaaaca atacaaaaat
     4681 atatagctct attgaaacta aataaagtcc agctcataag actaaataaa tatagagtat
     4741 tagctggcaa tcaagaatta aacttgatat aggtctacta cataaatact tttcaatata
     4801 aacacgatgg aacgcggagt gcggtgaaag tcgcacgctc cgtgtgaatg gggggaaaag
     4861 tttgagatat ttataatatc aggaacttac ctatccatat acacttggtt tttaagcaac
     4921 gcacagagtt tagtgcttat ggcgattgtg gttatcggta tctacttagg gtttaagcgt
     4981 gagttttcaa aacttatcgg cttcttggta gttgctcttg ttgccgttgg cttggtattc
     5041 aacgctggcg gtgtgaaaga cgtattgtta gagctgttca acaagattat cggtgcataa
     5101 aattttattg tagtgtgagt gtagaaatgg tatgatatgg atatgaagaa agcaacaaat
     5161 tagaagttgg aggaacaata atggactaca aagtagatga taaaaacctt gatgcatcaa
     5221 tgtttatttc ttttgtcaat caagtatggc agggcgatta tgatatggaa caaacaaggc
     5281 aagcattatc taaaacacta aacataacag cctatgacaa taaaaagtta gtagggtgtt
     5341 tgcgtattct ttccgatggt tactattttg gaacaattac agagctactt gttcttcctc
     5401 aatatcaaaa acagggtatt ggaagtaaac ttcttcaact tgcaaaagac aatacaccta
     5461 caatgttata tttcggttct caaccagaag cagagaagtt ttatgaaaag aacggctgtc
     5521 aaaaaagctt acaatcttac atgataaata agggataata agagataact tccagcttga
     5581 caatttcata tccatataca caaagcagtt agtcttagga ttgactgctt ttttcttacc
     5641 taaaaattag attggagtga tgtaatgaac gatattttta aggatatgca ggaaaaggtt
     5701 agctgtgaaa acatttcaga cctgccctct tacaagcgta aggtgtggca tgaaatgaag
     5761 caactgaaca ttgccgacta cgaagaaaaa cagttagaag atttttcaaa gtatgtgttt
     5821 ggtatgtcat accagacctt aaaagatgtg ataaaacagc agaaaggacg tgaggaacaa
     5881 tgcaggaaac aagggtgctg gtggaaacga gcggaacaac tggtgaagaa acaacgtcat
     5941 actggttcga cctgccaata gatgttgcag agtttgaaga aaagttaggt gtcggtgcag
     6001 aaagtcaaga ttaccgcatt atcgaaaagg tgttgcccta tgctgatgaa gtccatgaac
     6061 atacaagcgt ataccagctc aacgaattag attatatgta ccgccagctt tcaagtgata
     6121 tgcaggaaga atatgtatca ctacttacgg tgtatgaaaa tttagaagca ctttatattt
     6181 gcaggaacgt gattaccgtt tatcccgact gcaaaagcat gattgacgtt gcaagacaaa
     6241 agctgatgag cgacccgacg ttcaagcatt tatccgagga ttgtcaagaa tattattttg
     6301 actttgaagc ctacgcttct cacttgcagg aacacgggaa atttttagta acagaacacg
     6361 gtatctttga actgccagag taggaaatga ggtggtgctt atgattgatg atatggcggt
     6421 ttatattgca aacctcggta agtacaacga ggggtattta gtgggtgctt ggttcacatt
     6481 tcccattgac gaggaagatg tgaaagaaaa aatcggcttg aatgaacagt atgaagaata
     6541 cgcaatccat gataccgata acttccctat tgagattggg gagtatgttt ccattgaaga
     6601 actcaatgag atgtatgaaa tgataaagga acttcccgac tatatcgtgg attgtctgga
     6661 cgaatttatt ggtcattacg ggacgttgga agaagtcgtg gaacacaagg acgatattta
     6721 ctattatccc gattgtgaaa ccatgacaga tgttgcctat ttttacatag acgaattgca
     6781 ggcacttggg gacattcccc ctaacttgca gaactacatt gactatgaag catttgggcg
     6841 agatttggat atgggcggtt acttcattga aacaagccga ggtatgtgtg aaataccata
     6901 ttaacgctgg aagatcagcg acaaggattg ttgctactga cagcgtattt ctctttaagg
     6961 gacagtctga aaacggctgt ccttttccat ttcaaagaag ggagttgaaa aaaatttgaa
     7021 aaagattaaa tcttatacgg gtatctggaa cgtggaaaaa gtcttgtatg caatcaatga
     7081 ctttaactta ccctttcccg ttacctttac acagattaca tggtttgtga ttacggaatt
     7141 tatcatcatt ctgtttgggg atatgccccc actttccatg attgagggag catttttaaa
     7201 atacttcggt attcccgttg ctctcacttg gtttatgtcg cagaaaactt ttgacggaaa
     7261 gaagccgtac agctttttga aatcacagat aacctatgcc ctgcgaccta aaatcactta
     7321 tgcaggaaaa gccgtgaaac tggataagca gatactcaat gaaacaatca cggcagtaag
     7381 gagtgtgaac gatgttcccg ataaaatata ttgacaataa ccttgtctgg aacaaggaca
     7441 atgaggtgtt cgcctactat gagctgatac cctacaacta ttcttttcta tccgcagagc
     7501 agaaatttat cgtgcatgac agctttcgac agcttatcgc acagtcccgt gagggtaaaa
     7561 ttcatgcctt gcagatagcg acggaaagct caatccgcag tatgcaggag cagtcaaaaa
     7621 aactggtaac gggaaaatta aaggaagttg cctgccagaa gatagacgaa cagaccgaag
     7681 cgttggtatc tatgattggg gacaatcaag tggactaccg cttttttctt ggctttaagc
     7741 tcatggttac ggaagaacag ctcaatctga agaacatcaa aaaatcggcg tggctgacgt
     7801 tcacagaatt tctccatgaa gtgaaccaca cgctgatgaa tgacttcgtt tctatgccga
     7861 atgatgaaat caaccgttac atgaaaatgg aaaagttact ggaaaataaa atctcccgtc
     7921 gctttaaggt gcgtcgcttg gaaatcaatg attttgggta tctcatggaa catctttacg
     7981 gcagggacgg tatcgcctat gaagattatg agtaccagct accaaagaag aaattacaga
     8041 aagaaatgtt gataaaatac tacgacctta tccgtctgac aaggtgtgtg attgaggaaa
     8101 gccagcggta tttacgattg gaacatgagg acaaggaaag ctatgtgtcc tactttaccg
     8161 tcaatgcgat tgtcggcgag ctggattttc cgtcgtctga aatcttctat ttccagcaac
     8221 agcaattcac attccccgtt gatacttcta tgaatgtaga aatcgtggag aaccgtaaag
     8281 cattaacaac cgtccgcaac aagaaaaagg aattgaaaga ccttgacaat cacgcctatc
     8341 aagcaggaag tgaaacaagc tcaaatgtgg tggacgcatt agacagcgtg gacgagctgg
     8401 aaacggactt agaccagaca aaagaaagta tgtataagct ctcttatgta gtgcgtgtat
     8461 cggctgatga tttggacgaa ttaaaacgcc gttgtgatga agtcaaggac ttttacgacg
     8521 atttgaatgt aaagctggtg cgttctgctg gggatatgct ggggcttcat tctgaatttc
     8581 ttccagccag caaacgatat atcaacgact atgtgcagta tgtaaaatca gactttctag
     8641 ctgggcttgg ctttggtgcg acccagcagt taggggaaaa cacgggtatc tatatgggct
     8701 attccgttga tacgggaaga aatgtgtacc tgcaaccgtc ccttgccagt caaggcgtaa
     8761 aaggtacagt taccaacgct ttggcttctg cttttgtggg aagtcttggc ggtgggaaat
     8821 cattctgtaa taacctgctt gtgtaccatt ctgttttgtt tggcggtcaa gcggttatct
     8881 tagaccctaa atcagagcgt ggcaactgga aagaaacgct cccagaaatc gcccatgaaa
     8941 tcaatatcgt aaaccttacc agcgacaagg acaatgcagg acttcttgac ccgtttgtga
     9001 ttatgaagaa tgtaaaagac gctgaaagtc tggcaatcga catcttaaca ttccttacgg
     9061 gtatttcctc tagggacggc gaaaaattcc ccgtacttcg taaggcggtg cgttccgtta
     9121 cccagagtga aacaaggggc ttgctccatg tgatagacga gcttagaaaa gaagataccg
     9181 caatctcacg caatatcgca gaccatatcg acagcttcac ggactacgac tttgcacatc
     9241 tgcttttttc agacggtatg gtggaaaatg ctatcagtct ggataaccag ctcaatatca
     9301 ttcaagtagc agacttagta ttgccagata aagataccac ctttgaggaa tacacgacca
     9361 ttgaattatt gtcggtatct atgctgattg tcattagtac ctttgcttta gattttattc
     9421 acagcgacag aagcattttt aagattgtgg atttagatga agcgtgggcg ttcttaaatg
     9481 tggcacaagg cgaaacactt tcaaacaagc tggtgcgtgc tggacgagct atgcaggcag
     9541 gcgtttattt cgttacccag tctgcctatg atgtttcaaa agaaagtctg aaaaacaata
     9601 tcggcttgaa gtttgcattt agaagtaccg acatcaacga gataaaacag accttagaat
     9661 ttttcggtat cgacaaggac gacgagaaca accagaaacg cttgcgtgat ttggagaacg
     9721 gacaatgctt attgcaggac ttatacgggc gtgtcggcgt ggtacagata caccccgtct
     9781 ttgaagaact gctacacgcc tttgatacca gaccgcccat acagagaaat gaggtggagt
     9841 gatgaaaaaa aggataaaag gtgcgttcac aaaaaggaag atacttcact ttctcaaaat
     9901 ggctctgttc gtggtggcac tttcccttat tctgctttca cttttgggga cggtggctca
     9961 tgcaacggga cttgtggacg ataccataaa cgctgaaaat ctttattcca aatatccgtt
    10021 atccaactac cagcttgatt tttatgtgga taatagctgg tcgtggcttc cgtggaactg
    10081 gctggacggt atcggaaaat cggtgcagta cgggctttac tgcattacca actttgtctg
    10141 gactatcagt ttgtatttaa gcaatgcaac gggttatgtg gtgcaggaag cctataaact
    10201 ggattttatt aacgatatgg cagacagtat cgggcagagc atacagacct tagcaggcgt
    10261 aacacagaac ggtttttctt ctacgggctt ctatgtcggt ttcctgctac tcattatctt
    10321 agtggtggga atttatgttg catacacggg actgataaaa agagaaacca gcaaggcact
    10381 tcacgccgtt atcaactttg tggtggtgtt tgtattatcg gcttcattta ttgcctatgc
    10441 tcccgattac atcaagaaga taaacgaatt ttcaagcgac atcagtaccg cttcacttga
    10501 tttgggaaca aaaatcatgc tcccaaactc cgacagcgag ggcaaggaca gcgtggactt
    10561 gatacgggac agcctatttt ctattcaagt ggaacagccg tggctactct tacaatttgg
    10621 gaacagcaac gcagaggaaa tcgggacaga ccgtgtggaa gctctggtat cggtaagccc
    10681 agaggacgag gacggaaaaa ccagagagga aatcgtgaaa acagaaatcg aggataacga
    10741 caacaacatt ctgacgatac cgcaggtagt aaaccgttta ggtatggtgt ttttcctctt
    10801 gttcttcaac ttaggtatca cgatatttgt attcttgctt acgggcatga tgttgttcag
    10861 ccagatactt tttattatct ttgcaatgtt cctgcctatt agtttcctgc tctctatgat
    10921 accgagctat gaaagcatgg caaagcaggc aatcgtgagg gtatttaata ccataatgac
    10981 acgggcagga ataacgctca ttgtaacggt ggcgttcagc atttccagta tgttttacaa
    11041 tatctccaca gactacccgt tctttatggt ggcgttcttg cagatagttt gttttgcagg
    11101 tatctatatg aagctgggcg atttaatgag tatgttctca cttaatgcca gcgacagtca
    11161 aagcatggga cgcaggatat tccgcagacc gtatctgttt atgcgacata gagcgagaag
    11221 aatggagcga cgtatggcaa gggcggtaag cgctggcggt atttcgggcg gtgtcgctgg
    11281 tgcgctggct ggcagtaccg tttctaatgc aaggacaaca agaaacaatc ctacaaagga
    11341 aaatcggggc aataccactt ccagcatggg acagcgtgca ggctcaaaga taggcgctgt
    11401 cttagatacg aaaaataaag tgaaagacaa ggcaaatgct gtcaaagaga atgttaagga
    11461 tatgccgaca cagaccgctt atgcggtgta ttctgcaaaa gaaaaggcaa agtccagcgt
    11521 gtccgacttc aagcgtggca tggtgcagga acagcaatcc agacagacgg gacgcttaga
    11581 aaagcaggaa caacgtaaaa aaaatatcgc taacaagcgt atggagcttc aaaaggcaca
    11641 agaagcaagg caggcacgaa aggctgacgg atcagcgaca acgggagcta cccgtcccca
    11701 tgagcgacca gccacagctt caaagccgag tgtggaaaaa gtgcaggaag tcaaacgccc
    11761 tgccacaact ccgacttcaa aagcaagtga gccagtcaag acaaatgtta tcaaagagcg
    11821 tccgctatct tcgggtgctt ctgacggaaa agcaacccag tcggcacagc caatacatag
    11881 gcagaatata gaaaaagtgg tatcgcagga aacacgccag aattacgtta aagaccgcag
    11941 gacaaaggtt cagcagacgc agagcgtcca gaagaaccag cagaccacag agaaaaacca
    12001 taaccttgta acgaagaaag gacagaagaa aaaatgaaac tgaaacatat cgctcttatc
    12061 ggcagtctgt ttcctatcct cttttccata gtcctttttt tgggggtgct gattagtgcg
    12121 gacagcgacg acgaaaacag caacttttct tctggtatta cgggtttgaa cctatccgca
    12181 gaagtcttga aacatcagcc tatggtggaa aagtacgcca gagaatacgg catttccgag
    12241 tatgtcaatg tattactggc tatcattcaa gtagaaagtg gcggtacagc ggaagatgtc
    12301 atgcagtcca gcgaaagtct gggactaccg cccaactctt tagatacgga aagctcaatc
    12361 aagcaggggt gtaaatattt tgcgtccctg ctttcttcct acaaaaatca aggaataaat
    12421 gacttgaatg tggcgataca gtcctataac tatggcggtg actatgtggg atatgtggca
    12481 ggaaaaggga aaaagcatac gtttactctt gcagagagct tcgcccgtga aaaatcgggt
    12541 ggaaagaaag taacctacac caacccgata gccgttgcga agaatggggg ctggcgttat
    12601 ggctatggaa atatgtt
//
LOCUS       DBGJFF010000113        12542 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.115, whole genome shotgun sequence.
ACCESSION   DBGJFF010000113 DBGJFF010000000
VERSION     DBGJFF010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12542
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.115"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            134..922
                     /locus_tag="ACOIAF_002986"
     CDS             134..922
                     /locus_tag="ACOIAF_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HGZ2669987.1"
                     /translation="MATLIFFIVGIASLVVAFVIDGGHVGALFVFSSALVVFGGTIGA
                     VGASTPFPVFKKSIKIILLAFKNKKPNNIENIIYFKEIATKARKEGLLSIDKEISEND
                     ELDPFIRKGLELMVDGVDAVTIRNILESQIQITSKRHKEGIAIFDSAGGYAPTLGIIG
                     TVMGLVHILGNLSSNPDALGPQISVAFLATLYGVGSANLIWLPIASKLKAINQQEVSE
                     RLLVIEAILSILEGDNSNLMTEKLKIFLNSKELIELEEIGGRAE"
     gene            943..1683
                     /locus_tag="ACOIAF_002987"
     CDS             943..1683
                     /locus_tag="ACOIAF_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HGZ2669988.1"
                     /translation="MLYDEDEKEENNERWLLTYSDLITLLMIFFVIMYSMSNVDAEKY
                     KQLSQSLNSAFGGSSGVIEGGNSKIEPVVEPGSNDLDTLQNAKFKKVGEEIQKYLNEN
                     GMANSVSLRVQDRGLVISLKDTILFDTGKAIVKDNSRDKIIQIGKMLNEMNSYMRVEG
                     HTDNMSIKNSEFKSNWDLSVMRATSVVQLLIDNAGIAPDKLSAVGYGEFRPIAENSSE
                     EGRSKNRRVDIVLVDSKYDNVENVSKDK"
     gene            1859..2233
                     /locus_tag="ACOIAF_002988"
     CDS             1859..2233
                     /locus_tag="ACOIAF_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669989.1"
                     /translation="MGNKNKNTKYKDKTRERYNKKVENLNKEKLNKVNKQSKKVDKID
                     DIINKSSIVLCVLLVVFSLLYLFKLVQLNIIMILLVITVAIQQGLVSFGAFRNKNYVK
                     ALFSIFVIVCFLIAFKYVVNPV"
     gene            2325..3785
                     /locus_tag="ACOIAF_002989"
     CDS             2325..3785
                     /locus_tag="ACOIAF_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HGZ2669990.1"
                     /translation="MNRCKFAKLIITSMIVLLLTGCSLKKEDNYKLDPENPTSIQIWH
                     YYNGQQKMAFDKLVKEFNDTVGAEKGIIVEAFSQGNVVDLSEKVLDSANKKVGSEDVP
                     NIFAAYSDTAYEMDKLGLLVDMDKYISKEELNEYIDEYVEEGRINSKDEFKLFPIAKS
                     TEILMLNKTDWDKFAKATNSNINDIKTIEGLVETSKKYYEWTDSLTKEPNDGKAFFGR
                     DALANYIIIGSKQLGVEIFSVKDGKVTLNIDEKVMKKLWDNFYVPYINGYFASYGKFR
                     SDDAKTGDILALVGSSSGAAYFPDRVMLNDNESYNIETSVFQAPRFKGEGKYAVQQGA
                     GMAVTKSTKEEEYASVEFLKWFTDKEPNIKFSIASGYLPVKKKTNDLDVIKEVMKKDN
                     LSLSDKVEGSIASSIEQINSCNLYTNKAFDGGTKARQILEYSMLDKAKEDSNKINELV
                     KSGTSKKDAVAQFNNDKNFKTWFESFNAELENAIKK"
     gene            3838..6867
                     /locus_tag="ACOIAF_002990"
     CDS             3838..6867
                     /locus_tag="ACOIAF_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HGZ2669991.1"
                     /translation="MENNLRKNNSIMKKMIFPLIIVMLIQTSLFCATILWGGTIKKLN
                     DNSFDILNERVINRKNYIQNEMLQRWSNTSETEATINSSVKKVLNEKNALIEDVGNNE
                     EINKDIIKSISNDLIYLLRKNSVTGAFIILSDKNINDKSIDSKGEISRTGLYIRDLDP
                     KFNPNDNSDLLIERGSSDIARSFGISMDSYWEPKFSFKSENEQDGDDFFYKPFRAAID
                     NPNVSSSNLGYWGSKFLLTNAGDRLMTYTVPLIYEDGTVYGVLGIDLSVNYLRELLPY
                     SEINGNKKGNYILGVDRNNDMAFENVISNGYSLKEKFWDGLHSNLEQKESYKNIYKIK
                     NKENECGNGNIYASVQYLDLYNSNTPFENERWALLGIIEEKSLLSFSKRVEKLVIISI
                     AISLLIGLIGIYIASRLFTKPIVLLANKVRNSNPSKPVNLDKINISEIDELSSAIELL
                     SANVADSSSKLSQIIEMVDMPIGAFELYKNAENVFCTKEFFSVFTTEIKFEEYGYMEK
                     NKFLKKLRGLDKYLQESYETENTYIYKFESKDKKLKWIRMNIFEDELKVLGVVVDVTK
                     EFIEKRKIEYERDFDILTNLLNRRAFHSQIQEKFKNKDELKIAAFIMWDLDNLKYIND
                     TYGHDAGDEYIRCAADILKKFISWNGIVSRLSGDEFYVFIYGYESKDSIRDIINSVKK
                     QMNNTLLKLHDGTEFKIRASAGISWYPDDSTNYDELMRYADFAMYQIKNTIKGEVSEF
                     DINIYNKDSFLLSNREELNKLIDGQLVEYAFQPIVDCKGKVFAYEALMRPKIKTFSSP
                     IDVIRLARSQSKLYQIERITWFKALKSFKKYKEKFGDCKLFLNSIPNNNLSKVDFEEI
                     EDLYGEYLNRVVLEITENDKINEDFIEKKKAYIDHWNIEIALDDFGTGYNGDAVLLYI
                     MPDYVKIDMSIVRGIDKDENRQKILKNLISYSNERNIKVIAEGVETKGEMETLINLGV
                     DYMQGYYISKPSFIPEDISDKIKKELKICHENRNDVV"
     gene            6944..9010
                     /locus_tag="ACOIAF_002991"
     CDS             6944..9010
                     /locus_tag="ACOIAF_002991"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HGZ2669992.1"
                     /translation="MVELIITTNIDIRKLNENQLEAVEHIDGPCMILAGPGSGKTRVI
                     TYRIANMVVNRNIAPTRILAISFTKASSIEMKNRALNLSDDIRLNKVTYGTFHSVFFK
                     ILRYFERYNLDSIFDEKSKRMTIKAILKSLNIENADDDENIGQVINEISYVKNELMDK
                     NEFNSEVLTKDEFLKVYNLYEEQKSKVNKIDFDDMLIRTYYLLLNNKSALEMVRNVYK
                     YILIDEFQDINKVQFEVLKLICSPLNNIFAVGDEDQSIYGFRGARPDFLLEFEQYFNN
                     TKKIILDINYRSKSEIVHTANRLIDKNKNRYEKIIKCSQGDGGSVTYISPHDSEEEAL
                     YIAREIIDEIQKDYVEYSDFAVIYRTNIQSRALVDVFMDMRIPFVVKDSVITIYDHWA
                     SQDILAYLRIGINPKSNKDWLRIINKPFRYISKDSINMVKDEKDFITALINKCNLHPK
                     QVKTINDLEIDLSYLNTLNPKNAISYIRTSLDYDRYVLDYCSNRKIKTNGLVEILNEL
                     ESSATNFNTVTEFLEHIDRVKTELTENNKNKQTEGVIFTTMHSAKGLEFRNVYIIGVN
                     EGTIPHEKSYDICKEEKKEEQLEEERRLMYVAITRAEEKLCISSTLNKYGKKVDKSLF
                     INDIKSPTKKEIDSIGVGDKVYHKKFHEGEIIKKDGIMFTIKFKDRERILDLKTCLLK
                     NIIYTI"
     misc_binding    9435..9645
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            9871..10686
                     /locus_tag="ACOIAF_002992"
     CDS             9871..10686
                     /locus_tag="ACOIAF_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HGZ2669993.1"
                     /translation="MKVFKKLLSLGLVLGLTLSLVGCSGGGEKTKLEQIKDNGKLVVG
                     TSAEFPPFEFHKVVDGKDSIKGFDIMLAEEFAKELGVKVEIKDMSFDGLIGALNADQV
                     DIVLAGMSPTPEREKSVDFSELYYLSRNAVIVKDADIDKVKTEDDLKKLRVGVQAGSI
                     QEEYVVNTLKMTTTKSLKAIPDLITELKNGNIDAVVTNEAVSLINVKKYDGIKMANTE
                     VGKDVTEGMAAAIKKSDNNKDFIELLNKKIKELQDGKKIEEFLNEASTEAASN"
     gene            10714..11391
                     /locus_tag="ACOIAF_002993"
     CDS             10714..11391
                     /locus_tag="ACOIAF_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HGZ2669994.1"
                     /translation="MSLDFSFLSRFGTSFLEGTGVTVSISLVALCFGFIIGIIICMAK
                     ISKSKVLRAISSIYIEVLRGTPLLVQIYIVWFGLPQLGIRFPMLFGIPSEFIASAFAL
                     SVNSGAYVAEILRSGIQSVDNGQMEASRSLGLNYWSTMRYIIIPQAIKNILPSLANEF
                     ITLVKESSIISVIGVVEIMRTADIVKNAAFRALEPLIVAAAIYFVITFTLSRLVGLLE
                     KKLSVSN"
     gene            11450..12172
                     /locus_tag="ACOIAF_002994"
     CDS             11450..12172
                     /locus_tag="ACOIAF_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HGZ2669995.1"
                     /translation="MITIKNLSKSFGDLNVLKNIDLEIAKGEIMVIVGPSGSGKSTLL
                     RCMNLLEIPTGGEIIFEGKNLVDKKTNIDEVRQNIGMVFQNFNLFPHKTILDNITLAP
                     IKLKKMTKEEAEKKAEILLSRVGLLDKKDSYPSQLSGGQKQRIAIARALAMEPDMMLF
                     DEPTSALDPEMVNEVLDVIKELAKEGMTMAIVTHEMGFAKEVADRVIFIDGGSILEDN
                     TPEEVFGNPKHERTKAFLAKVL"
BASE COUNT         5065 a         1225 c         2273 g         3979 t
ORIGIN      
        1 aggctgggta accggtgcga gggttcgaat ccctccttct ccgccaatta ttaaaggcta
       61 tttttattaa agatagtctt tttataatgt cataaatgat tagatatgta taactaaaga
      121 aaggagggaa tagatggcaa ctttaatttt tttcatagtg gggatagcat ctttggtagt
      181 tgcttttgtt attgatgggg gacatgttgg agcattattt gtattttcat cagcacttgt
      241 tgtgtttggt ggaactatag gagctgttgg agcttcgact ccattccctg tgtttaaaaa
      301 gtctataaag ataatattat tagcttttaa aaataaaaag cctaataaca tagaaaatat
      361 aatctatttc aaagaaattg caacaaaggc taggaaagag gggcttctta gtattgacaa
      421 agaaataagt gagaatgatg aattagaccc ttttattaga aaagggcttg aacttatggt
      481 agatggagtg gatgcagtta ctataagaaa tatattggaa tcacagatac agataacatc
      541 aaaacgacat aaagaaggaa tagcaatttt tgattcagca ggtggatatg cacctacact
      601 ggggataata ggtacagtta tgggattagt acatatttta ggtaatcttt caagtaatcc
      661 agatgcatta ggacctcaaa tttctgttgc attcttggct actttgtatg gtgttggttc
      721 tgccaattta atatggttac caatagcatc aaaacttaag gctataaatc aacaagaagt
      781 aagtgaaaga cttttggtga tagaagcaat attatccata cttgagggtg ataattctaa
      841 tttaatgaca gaaaaattaa aaatattttt aaattcaaaa gaactaattg aacttgaaga
      901 aattggtggt agagctgaat aatataaaag gatggaatgt ttatgttgta tgatgaagat
      961 gaaaaagaag aaaacaatga gcgatggctt ttaacttatt cagacctcat aacattactt
     1021 atgattttct ttgtaataat gtattctatg agtaatgttg atgcggagaa atataaacaa
     1081 ttatctcaat ctctaaattc tgcttttggt ggttcttctg gagttattga aggtggtaat
     1141 agcaagatag aaccagtagt tgaaccagga agtaatgatt tagatacttt acaaaatgct
     1201 aagtttaaaa aagttggtga agaaatccag aagtatttaa atgaaaatgg tatggctaat
     1261 tcagtatcat taagagttca agatagagga ttagtaataa gccttaaaga tacaatacta
     1321 tttgatacag ggaaagcgat tgtgaaggat aattctagag ataagattat tcaaataggt
     1381 aagatgttaa atgaaatgaa tagttacatg agagtagaag gccatacaga caatatgtct
     1441 ataaaaaatt cagagtttaa gtcaaattgg gatttatcag ttatgagagc aaccagtgtt
     1501 gttcaacttc ttattgacaa tgcaggtata gcaccagata aattatctgc tgtaggatat
     1561 ggtgagttta gaccaatagc agaaaattca tctgaagaag gtaggtcaaa aaatagaaga
     1621 gttgatatag ttttagttga tagtaaatat gataatgtag aaaatgtttc aaaggataaa
     1681 tagttaaatt agctgtctca atacagaatt aattctaaag gagatggctt ttttatgcga
     1741 aaatcaaaaa tagatacata gtaagttacc tttttgtagg aatatatggt atcttaaata
     1801 tataatattt aaataatgta taagctcaat taaaaaattt atagaggatg gaaaataaat
     1861 gggaaataaa aataaaaata ctaaatataa agataaaaca agagaaaggt ataataaaaa
     1921 agttgagaat ttaaataaag aaaaattaaa taaagttaat aagcaatcta agaaggtaga
     1981 taagattgat gatattatta ataaatctag tattgtatta tgtgtacttc tagtagtgtt
     2041 ttcattgctt tacttattta aactggttca acttaatata ataatgatac ttttagtaat
     2101 tacagtagct attcaacaag gattggtgtc atttggtgca tttagaaata aaaattatgt
     2161 aaaagcttta ttttctatat ttgtaatagt atgtttctta attgctttta aatatgttgt
     2221 aaatcctgtt taaatatatc aataaacgtt ctataattaa tataaatagt gaagttacag
     2281 ttttaatgct attataaatt ttataatttg aaggagtaga ttttatgaat aggtgtaagt
     2341 ttgcaaaatt aattatcaca tcaatgattg ttttattact tacaggatgt agtttaaaga
     2401 aggaagataa ttataagtta gacccagaaa atccgacatc aatacaaatt tggcattatt
     2461 ataatggaca gcagaaaatg gcttttgata agcttgttaa ggaatttaat gacactgtag
     2521 gtgctgaaaa aggaattatt gttgaagcat tcagtcaagg taatgtagtt gatttatcag
     2581 agaaagtttt agattctgca aataaaaaag ttggttctga agatgtacca aatatatttg
     2641 cagcatactc agatactgca tatgaaatgg ataaattagg gttactagta gatatggata
     2701 agtacataag taaagaagaa ttaaatgaat acatagacga atatgtagaa gaaggcagaa
     2761 taaattctaa ggatgagttt aaacttttcc caattgcaaa atcaactgaa attttgatgt
     2821 taaataaaac tgattgggat aaatttgcaa aagctacaaa ttcaaatata aatgatataa
     2881 aaacaattga aggattagta gagacatcaa aaaaatatta tgaatggaca gatagtttaa
     2941 cgaaagagcc taatgatggt aaagcatttt ttggaagaga tgctttagca aattatatta
     3001 ttataggaag taaacaacta ggagtagaaa ttttttctgt taaagatggt aaagttacac
     3061 taaatattga tgaaaaagtt atgaaaaaac tatgggataa tttctatgta ccatatataa
     3121 atggatattt tgcttcttat ggtaagtttc gttctgatga tgcaaaaaca ggtgatatat
     3181 tagctcttgt aggttctagt tcaggagcag catactttcc agatagagta atgctaaatg
     3241 ataatgagag ttataatatt gaaacatctg tttttcaagc accacgtttt aaaggagaag
     3301 gaaaatatgc tgtacaacaa ggtgcaggaa tggctgtaac aaaatctact aaagaagaag
     3361 aatatgcatc agtagaattt ttaaaatggt ttacagataa agaacctaat ataaaattct
     3421 ctattgcttc tggatactta ccagtaaaga aaaaaacgaa tgatttggat gttataaaag
     3481 aagttatgaa gaaagataat ttgagtttat ctgataaagt tgaaggttca atagcctcat
     3541 ctattgaaca gattaacagt tgtaatttat atactaataa agcatttgat ggtggaacta
     3601 aagctagaca aatacttgaa tattcaatgc ttgataaagc taaagaagat tcaaataaaa
     3661 taaatgaatt agtaaaaagt ggaacttcta agaaggatgc agtagcacaa tttaataatg
     3721 ataaaaattt taaaacttgg tttgaaagtt ttaatgctga gcttgaaaat gctataaaaa
     3781 aatagatgaa ttggcagtta gtgtgttgac gagcacaaag gtataggtgg aatgggtatg
     3841 gaaaacaatc taagaaaaaa caattcaata atgaaaaaga tgatatttcc cctaattatt
     3901 gttatgctaa ttcaaacaag tttattttgt gccacaattt tatggggagg aactattaag
     3961 aaattaaatg ataattcgtt tgacatctta aatgaaagag taattaatcg taaaaattat
     4021 atacaaaatg aaatgttaca aagatggtca aatacaagtg aaacggaagc tacaattaat
     4081 tcctctgtaa aaaaagtatt aaatgaaaaa aatgccttaa ttgaggatgt aggcaacaat
     4141 gaagaaatta acaaagatat tataaaaagc atatcaaatg atttaatata tcttttgaga
     4201 aaaaattctg ttacaggagc ttttataatc ctaagtgaca aaaatattaa cgataagtca
     4261 atagatagta aaggtgaaat aagtaggaca ggattgtata taagggattt agaccctaag
     4321 tttaatccaa atgataattc tgatttgtta attgagagag gttcatcaga tatagcaaga
     4381 agttttggaa tatctatgga cagttattgg gaacctaagt tttcatttaa aagtgaaaat
     4441 gagcaagatg gagatgattt tttttataaa ccatttagag ctgcaattga taatccaaat
     4501 gtatcaagtt ctaatttagg atactgggga agtaagtttt tattgacaaa tgcaggtgat
     4561 aggcttatga catatacagt tccccttatt tatgaagatg gaactgtata tggagtactt
     4621 ggaatagatt taagtgtaaa ttatttaaga gaattacttc catactcaga gataaatggg
     4681 aataaaaagg gtaattatat tttaggtgta gatagaaata atgatatggc ctttgaaaat
     4741 gtaatttcaa atggatactc attaaaagaa aaattttggg atggtttaca ttctaattta
     4801 gagcagaagg aatcgtataa aaatatatat aagattaaaa ataaagagaa tgaatgtggt
     4861 aatggcaaca tttatgctag tgtacaatac ttggatttat ataactccaa tactccattt
     4921 gaaaatgaac gttgggctct tttgggtatt attgaagaaa aaagtctttt gtcattttca
     4981 aaaagggtag agaaattagt tataatatct atagcaattt ctttattaat aggtctaata
     5041 ggtatttata ttgcatcaag attatttaca aaaccaattg ttcttttagc caacaaagtt
     5101 agaaacagta atccatcaaa accagtaaat cttgataaga taaatatatc tgaaatagat
     5161 gaattatctt cagctataga gttgctcagt gcaaatgttg cagattcttc gtcaaaatta
     5221 tcacagatta ttgaaatggt agatatgcca attggggctt ttgagttata caaaaacgca
     5281 gagaatgttt tttgtacaaa agaatttttt agcgtgttta ctactgaaat caaatttgaa
     5341 gaatatggat atatggaaaa aaataagttc ctgaaaaaat taaggggact agataaatat
     5401 ttgcaagaaa gttatgaaac agaaaataca tatatatata agtttgaatc taaagataaa
     5461 aaacttaagt ggataagaat gaatatcttt gaagatgagc taaaggttct tggtgttgtt
     5521 gttgatgtaa caaaagaatt tattgagaaa aggaaaatag aatatgaaag agattttgat
     5581 attttaacga atcttttaaa taggagagct tttcattcac agatacaaga aaaatttaag
     5641 aataaagatg aacttaagat agcagcattt attatgtggg atttagataa tctaaaatat
     5701 attaatgata catatggaca tgatgctggt gatgaatata ttaggtgtgc tgcagacata
     5761 cttaagaagt ttatatcatg gaatggtata gtatctagac tttcaggtga tgagttttat
     5821 gtatttatat atggatatga aagcaaagat agtataagag atattattaa ttctgttaag
     5881 aaacagatga ataatacctt attaaaactt catgatggaa cagaatttaa aataagggca
     5941 tcagcaggaa tttcctggta ccctgatgat tcaaccaact atgatgaatt aatgagatac
     6001 gcagattttg ctatgtatca aattaaaaat acaatcaagg gtgaagttag tgaatttgat
     6061 attaatatat ataataagga ttcattctta ttgagtaata gagaagaatt aaacaagtta
     6121 attgatggtc aattagttga gtatgcattt cagcctattg tagattgtaa aggtaaagtt
     6181 tttgcatatg aagcacttat gagacctaaa ataaaaactt ttagttcacc aatagatgtc
     6241 attagattag ctcgttcaca gtctaaatta tatcaaattg agcgtattac atggtttaaa
     6301 gcacttaaat catttaaaaa atacaaagaa aaatttggcg attgcaaact gttcttaaat
     6361 tctattccta ataataattt gtcaaaagta gattttgagg aaattgaaga tttatatggt
     6421 gaatatttaa atagagtagt actagaaata acagagaatg ataagattaa tgaagatttt
     6481 attgagaaaa agaaagcata tatagaccat tggaatatag aaattgcttt agatgacttt
     6541 gggacaggtt acaacggaga tgctgtacta ttatatataa tgccagacta tgtaaagatt
     6601 gatatgtcta ttgtaagagg tattgataaa gatgaaaaca gacagaaaat attaaaaaat
     6661 cttatctcat attctaacga aagaaatatt aaagtgatag cagaaggtgt agaaacaaaa
     6721 ggtgaaatgg aaacactaat taatcttgga gtagattata tgcaaggcta ctatattagt
     6781 aaaccatcat ttatacctga agatataagc gacaaaatta aaaaagaact caagatatgt
     6841 catgaaaata ggaatgatgt ggtttaatag aggaaaaatt aaatttattt ttatgaactt
     6901 ttagtttata attaaaaaga aaataatata attataaatc aaggtggtgg agctaataat
     6961 cacaacaaat atagatataa gaaagttaaa tgaaaatcaa ttagaagcag ttgagcatat
     7021 agatgggcct tgcatgatac ttgcagggcc tggttcaggt aagaccagag taattacata
     7081 tagaatagcc aatatggttg taaacagaaa catagcacca acaagaatct tggcaataag
     7141 ttttaccaag gcatcttcaa tagaaatgaa aaatagagct ttgaatttaa gtgatgacat
     7201 aagacttaat aaggtaacct atggtacttt tcactctgta ttttttaaaa ttctaagata
     7261 ctttgaaaga tacaacttag acagtatatt tgatgaaaag tcaaagcgaa tgacaatcaa
     7321 ggcaatccta aaaagtttaa atatagagaa tgctgatgat gatgagaata taggacaagt
     7381 tataaatgaa atttcctatg taaaaaatga acttatggat aaaaatgaat ttaattcaga
     7441 agtattaaca aaagatgagt ttttaaaagt atacaactta tacgaagaac aaaaatctaa
     7501 agttaataaa atagattttg atgatatgct tataagaact tattatttac ttttgaacaa
     7561 taagtctgca ctagaaatgg ttagaaatgt gtataaatat atattgatag atgaatttca
     7621 agatataaat aaagtacagt ttgaagtttt aaagttaatt tgtagcccat taaacaatat
     7681 atttgcagtt ggagatgaag accaaagtat atatggattt agaggtgcta gaccagattt
     7741 tttgcttgaa tttgaacaat actttaataa tactaaaaaa ataatattag atataaatta
     7801 caggtctaaa agtgagatag ttcatactgc aaatagactt attgacaaaa ataaaaatag
     7861 atatgaaaaa atcattaagt gtagtcaggg ggatggagga agtgttacct atatttctcc
     7921 gcatgattca gaggaagaag ccttatatat tgccagggaa ataattgacg aaatacaaaa
     7981 agattatgta gagtattcag attttgcagt aatttataga actaatatac agtcaagagc
     8041 attagtagat gtatttatgg atatgaggat tccatttgta gtaaaagact cagtaattac
     8101 aatatatgac cattgggcaa gtcaagatat actagcctat ttaagaatag gtattaatcc
     8161 aaaatcaaat aaagattggt tgagaattat aaacaagcct tttagatata tatccaaaga
     8221 cagtataaat atggttaagg atgaaaaaga ctttataaca gcattaataa ataagtgtaa
     8281 tcttcatcct aaacaagtta aaacaataaa tgatttggaa atagatttaa gttatttaaa
     8341 tactttaaat cctaaaaatg caatatctta tattagaact agtcttgatt atgatagata
     8401 tgttttagat tattgtagca acagaaagat aaagactaat ggacttgtag agatattaaa
     8461 tgaacttgag agttcagcaa ctaattttaa tactgttaca gagtttttag agcatattga
     8521 tagagtaaag actgagttaa cggaaaataa caagaataag cagactgagg gtgtcatatt
     8581 tacaactatg catagtgcaa aaggattaga gtttagaaat gtttatatta taggtgtaaa
     8641 tgaaggaaca atacctcatg aaaaatcata tgatatttgt aaggaagaga aaaaagaaga
     8701 acaattagaa gaagaaagaa gacttatgta tgttgctatt acaagagctg aagaaaagtt
     8761 atgtataagc tctactttaa ataagtatgg taagaaagtt gataaatcat tatttattaa
     8821 tgatataaaa tctccaacga aaaaagaaat tgacagtatt ggtgtagggg ataaggtata
     8881 ccataaaaaa tttcatgaag gagaaataat taaaaaagat ggaattatgt ttacaataaa
     8941 gtttaaagat agagagcgaa tcttggattt gaaaacttgt ttattgaaga atataattta
     9001 tactatttaa tttagaatct aaatttagat ataaatataa tgtattattt attatgtgca
     9061 agacaaatag atatgtgtaa aatgtattat ttaacttatt agaaaaagaa ataagggttt
     9121 aatatattat ttatagttgt ttagaaaaag caacaatgtg gaaaatatta tttagtatta
     9181 ttaaagaaaa gcaacaatgt gaaagatatt atttagtatt gtttaagaaa aagcaacaat
     9241 gtgaaagata ttatttagta ttatttaaga aatagctaaa aatatgaaat atactattta
     9301 taattacaca aataatatca ataaatataa acagtagtat ttagataatt tacaagtaaa
     9361 aattattaaa aaaataaaaa acttattgac aaaaatatta taaggaagta caattgtatt
     9421 aacaatttaa ggaaacaaat ccaatgatag agaaagtagc atatatggat ttacagcgag
     9481 ttaggggttg gtgtaagcct agcaatgaag tctatgtgaa gagagctctg gaggagatta
     9541 tcagaaactt tagtatgata attggtttac ccgccttaag ggattgagag gataagtact
     9601 tacttatcaa aaagagtggt aacgcggata taattcgtct cttagctgta aagctaaggg
     9661 acttttttga ttttaaaata aaaatatcca tacagcttaa aagctaacag tcacaaattt
     9721 tgaaagtaat ataagtattt atcaataaga gtggtaacgc ggtaaagtcg tctcttagtc
     9781 attaaagact gagagacttt ttttgtaact cgaaacattt aagtattaaa atctgaggga
     9841 aattataatt aaaagagagg ggtagttaaa atgaaagtat ttaaaaaatt attaagttta
     9901 ggattagtat taggattgac attatcgcta gtaggatgta gtggtggagg agaaaaaaca
     9961 aagttagaac aaataaaaga caatggaaaa ttagttgttg gtactagtgc agaatttcct
    10021 ccatttgaat ttcataaggt agtagatgga aaagattcaa taaaaggatt cgatataatg
    10081 ttggctgaag aatttgcaaa agagcttggt gtaaaggttg aaattaagga tatgtcattt
    10141 gatggattaa taggtgcttt aaatgcagac caagttgata ttgttcttgc aggtatgtct
    10201 ccaacaccag agagagaaaa gagtgttgat ttctcagaat tatattattt aagtagaaat
    10261 gcagttatag ttaaagatgc agatatagac aaagtaaaaa ctgaagatga tttaaagaaa
    10321 cttagagttg gagttcaagc aggaagtatc caagaagaat atgtagttaa cactttaaaa
    10381 atgacaacta caaaatcttt gaaagcaata cctgatttaa ttacagaatt aaaaaatgga
    10441 aacatagatg cagttgttac taatgaagca gtttctttaa taaatgttaa aaaatatgat
    10501 ggaataaaaa tggcaaatac tgaagttggg aaagatgtaa cagaaggtat ggcagcagca
    10561 atcaaaaaat ctgacaataa caaagatttt atagaattat taaacaaaaa gataaaagaa
    10621 ttacaagatg gcaagaagat agaagaattt ttaaatgaag catctactga agctgcttca
    10681 aattagtaag aaagttatgt gggagatgaa attatgagtt tagattttag ttttttaagt
    10741 agatttggga cttctttttt ggaaggaaca ggtgtgacag tatcaatttc tcttgtggca
    10801 ttatgctttg gatttataat aggtataatt atctgtatgg caaaaatatc aaagagcaaa
    10861 gtattaagag caatatcatc aatctatatt gaggttttaa gagggacacc attattagta
    10921 cagatatata tagtatggtt tggtttacct caattaggaa taagattccc tatgttgttt
    10981 ggtataccaa gtgaatttat agctagtgca tttgcattat cagtaaactc tggtgcatac
    11041 gtggcagaga tacttagatc aggtatacaa tcagttgata atggacaaat ggaagcaagt
    11101 agatcattag gattaaatta ttggtcaact atgagatata taataatacc tcaagctata
    11161 aaaaatatat taccttcatt agcaaatgag tttattacac ttgtaaaaga atcttcaata
    11221 atatctgtaa taggagttgt tgagataatg cgtactgctg atatagtaaa aaatgcagca
    11281 tttagagcat tagagccatt aatagtagca gcagcaattt actttgttat aacatttact
    11341 ctatcaagat tagttggatt attagaaaag aaactatctg tgtctaacta gatgagagca
    11401 tgtttagcat ttagggaaaa catagaatag taaaaaggaa agtgataata tgattacaat
    11461 aaaaaattta agtaaatctt ttggagattt aaatgtatta aaaaatatag acttagaaat
    11521 cgctaaaggc gaaataatgg ttatagtagg tccaagtggt tctggtaaaa gtacacttct
    11581 tagatgtatg aatttactag aaatacctac aggtggagaa ataatatttg aaggaaaaaa
    11641 tttggtcgat aaaaaaacta atattgatga ggttagacaa aatataggta tggtgtttca
    11701 gaactttaat ttattcccac ataagactat tttagataat ataacattag caccaattaa
    11761 attaaaaaag atgactaagg aagaagcaga aaaaaaagca gaaattttgt tgagtagagt
    11821 tggtctttta gataaaaaag attcatatcc atcacaatta tctggaggac aaaagcaaag
    11881 aatagcaata gctagagcac ttgctatgga accagatatg atgttgtttg atgagccaac
    11941 atctgctctt gacccagaaa tggtaaacga agttttggat gttataaaag aacttgctaa
    12001 agaaggtatg acaatggcaa ttgtaactca tgagatggga tttgctaagg aagttgcaga
    12061 tagagttatt tttatagatg gaggaagtat acttgaagat aatactccag aagaagtttt
    12121 cggaaatcca aaacatgaaa gaacaaaagc atttttagca aaagtgctat aatatataaa
    12181 aaggatgtta ttgatattag aaattcaata catccttttt tagtgatggt taatattaat
    12241 tttaaaatta aagttatatt aatattaact ttatttttac gattttttgc agaaaaaaaa
    12301 agagctcggg gtaagctctt tttttccacc gaattaattt ccgttactaa tccgattttt
    12361 aataaatata aaatatatcc ttatgaaagg gtattgggaa tagatatatt aactaactag
    12421 ggttaccagg gggataacct taatttatta taacaaaatg cgacacaaaa atcaatattt
    12481 tttttgaaat aaattataaa agttattttt ataacaagta agcttataaa tttaactttt
    12541 at
//
LOCUS       DBGJFF010000114        12526 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.116, whole genome shotgun sequence.
ACCESSION   DBGJFF010000114 DBGJFF010000000
VERSION     DBGJFF010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12526
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.116"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(348..893)
                     /locus_tag="ACOIAF_002995"
     CDS             complement(348..893)
                     /locus_tag="ACOIAF_002995"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGZ2669996.1"
                     /translation="MRVEPIENKKDIEKLKRYFRNKGKIRDLTLITLGLNTGFRIEDI
                     LKLRVKDVYKKREINIKESKTGKRIRKPINDSLKEILDIYCKDKPTNEYLFKSQKGEN
                     EAISTTQAYRIIKKASKACRIKLNIGTHSMRKTHGLHIFKDKGIEVAQRALNHANQID
                     TLTYIGVVQKQADNAIMKLNL"
     gene            complement(909..1157)
                     /locus_tag="ACOIAF_002996"
     CDS             complement(909..1157)
                     /locus_tag="ACOIAF_002996"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669997.1"
                     /translation="MEGTKRSYRKIPKLDLRKVKLADVFEKNKSGVFRVEEADEQLLH
                     ECLDETKSIREEKHLTWQIQFKGKSVYCVFYEHPFWDL"
     gene            complement(1144..2079)
                     /locus_tag="ACOIAF_002997"
     CDS             complement(1144..2079)
                     /locus_tag="ACOIAF_002997"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2669998.1"
                     /translation="MINIIDLRFYLRNDLFTNEELTEITKQITEENVFLYDIAEEKGC
                     HPYTLNKCLQRFEHSSMEVGKFIGEMSELHKEGALTTEELKLKFEIVKEILILIKRSE
                     EAEKILHLLENEKVKTVAEKLYKSRSSVYDILKQMGFKKNKYINVWYHIDNENIAGNV
                     ENTKRDVELVHLYQQRYDNFLKNYYLDSEIIDIDKNILDDIETLSEEFEYETVNDFIS
                     VLLLRGLQNERFKNKENINNKNDNNNIVKMKDVEHEIFFTQGAEMLEEYYKTNLIDVH
                     KISKTEIEEMNPKELMELYHLKNEGKAKEDFYGRD"
     gene            complement(2250..2897)
                     /locus_tag="ACOIAF_002998"
     CDS             complement(2250..2897)
                     /locus_tag="ACOIAF_002998"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HGZ2669999.1"
                     /translation="MIIGYERVSTRKQDNSRQERVLENEGIKFDKIYRDKVTGATKER
                     EALNRMLLEVNEGDTVYCESISRLGRNLKDLIDIIDTLVQKGVRVKILKEGIDTGNNT
                     YKLLLGIFGAVAEMERETIQERTQQKIDQLKEDKKNTGEINTKSGKWFGRQKVTKELL
                     ETNKEFIKYYNKILDGSLKKNEVAKILNVGRTTLYEWIKVYEGGEIKRSNRAKKN"
     gene            complement(3487..3855)
                     /locus_tag="ACOIAF_002999"
     CDS             complement(3487..3855)
                     /locus_tag="ACOIAF_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGZ2670000.1"
                     /translation="MELDFKFIGQRIKIARIKKKLTQEVLSEKINVSPQHVSNIETGN
                     SSVSLPTLVAIANTLGVSVDELLCDTILSSKAVFENELEEVLRDCNEYEIRFITDIVK
                     ASKKSIRDIKLFQSNLNDRY"
     gene            3971..4303
                     /locus_tag="ACOIAF_003000"
     CDS             3971..4303
                     /locus_tag="ACOIAF_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HGZ2670001.1"
                     /translation="MDIPKIPQTELKVMKFIWSKNDIVTSKEVAKAMELEYSWKMTTT
                     LTILSRLVIKHFLASERIGRMTHYTILIAKKDYKISETKRFLNDIHSNSLESLLNSLK
                     DCNITHKK"
     gene            complement(4451..4888)
                     /locus_tag="ACOIAF_003001"
     CDS             complement(4451..4888)
                     /locus_tag="ACOIAF_003001"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670002.1"
                     /translation="MRVKNIISIFVCALIIIMSVNVVSFAEQFNSSDIIFMEDGKEEI
                     NPFSVYILNGRCNLSFSNGKANVNASVVGKSGVTRTSIVTKLQELKGGSWVNLKTSTK
                     KTGGKTCSVLNSYSVTKGHTYRAVAIITAENESKTLISTSQKY"
     gene            complement(4957..5658)
                     /locus_tag="ACOIAF_003002"
     CDS             complement(4957..5658)
                     /locus_tag="ACOIAF_003002"
                     /inference="COORDINATES: protein motif:HMM:NF025643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4367 domain-containing protein"
                     /protein_id="HGZ2670003.1"
                     /translation="MNKDKLDIEKLLYEQLPKIEEKILYELPCDEDIDYEFSIEFESK
                     MDKIIKENEKKVKKNTYKNLKKIAIVFIIILSSIVTITTIKTEAFPENLIRIMKKVYK
                     EFTDYNFINPHKSNVSFKYIEPKYISTGYKEVERIEEENYLSILYKDKLDNYIIYQMM
                     LYSRDVIVLDTEDAFVENIKFNNQYAQYVEKGNTQQLLWHDKNNIYMLSKELPTKKIT
                     KKDKKEFIKIAKNIK"
     gene            complement(5651..6196)
                     /locus_tag="ACOIAF_003003"
     CDS             complement(5651..6196)
                     /locus_tag="ACOIAF_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HGZ2670004.1"
                     /translation="MLIYLAIIDSKEERIKFEEIYIKYKKLMFYVANQILKDDVLAED
                     AVHSSFLKIIDNLDKVNDVNSPKTKGFVVIIVKRISINIYNRRKKEEISNINDDNYKF
                     SSLDLSIEKLGECSYLGEALKKLSETDLQVILLKYSHGFSIREISKLLNIKEVNLYKR
                     NKRALEKLKKILSEMEALSNE"
     gene            6332..7060
                     /locus_tag="ACOIAF_003004"
     CDS             6332..7060
                     /locus_tag="ACOIAF_003004"
                     /inference="COORDINATES: protein motif:HMM:NF039973.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670005.1"
                     /translation="MKSKRINKMLATTMTMAMLAIPAVTATSYAMESTGLGTEYTYNN
                     ESKQQEEWVKSFDTETGKEILLNKTTNEKVSEVYENVNGKLVKLSINEALEKFNLNEE
                     IITKSPKSIFGVSSVKKITGSARKISQDVSGGKQGATISIGNSTTVTESFTVGGTTEA
                     IKAAVRANAGFTWSKSLTRSVTTTHKVPAGKIGYVAFKPYYKEVRGTVNGILAGTSIT
                     QKIVAKSPLKAGKVCDGLEYLAYK"
     gene            7086..7334
                     /locus_tag="ACOIAF_003005"
     CDS             7086..7334
                     /locus_tag="ACOIAF_003005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670006.1"
                     /translation="MENKNMYLRVSLILAVLGFIILFFNNDLALSLKATYLADKGFED
                     IVENQILKNYSYMFLIIGGVLFSIGIYNLTKLKQINKK"
     gene            complement(7520..10129)
                     /locus_tag="ACOIAF_003006"
     CDS             complement(7520..10129)
                     /locus_tag="ACOIAF_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011572948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HGZ2670007.1"
                     /translation="MKIDIKQLIDIIKNANIDELYLRDIGVDTTQKRNVPCPIHGGKD
                     KNFQFDLNKRIYTCYSHNCVVGADIIELCRTYERFERPIEAILFLANKYNISLPINKS
                     KTNIKKTNNTIKNIYIKKKDINSFIEEKKQEAFKNNDIELAFEFECMTDKDKRKYYLD
                     NNIKIKSMLEKQNYSSYKADSIINIDKYISENLKGIRESIKHATEGKNVLMVAPTGSG
                     KTYSIINTLKELDIKALFIFPNSANVQQAIVEYKIAGAYDKIPTKHALEGNKLVAMTW
                     DKTEQLLKEDLSEYIIVIDEIHQTYTDAFRSKAIKSLNNITNKCRGRLDITATPSKLE
                     FEIYNKVIEYKQNIQTEYKVKLYDKINIGKVIEILNNSNNSMLLKDSISTLNYISKKV
                     NKKSGVVISDTKDTSKLYDRIVSYSDMEGYEVLLNTSVITAGVNINNPNVTDIIVIGE
                     KDVGKIKQYVARARGAKSINVHIFNSYKTTNEDSNVYSVEWCINENIKDVESLTNIYN
                     KASKRDLPYRAIGIDISPINIKNIDSNIYYDKKDMCYKTDKLYIKSNAYNNYYQTRTI
                     NSFKVLLEEYFNKIEIVETEKETKKIEEEIKEHEEAIKEFKKSAIEQLEGHKKYLVGY
                     SEIKKSMTSSNLLKYHHDMRIDNNICLKYYMEHDLYSLIINNNCRKTIDLFSDFVLDN
                     SYSIDLSWKLATMSDEKRNLFFEQINTTIYRKINKKYANSLLNDDNIGTYTYNYINFN
                     FGVGTSYTKEHLEALAVDLQTFLATKKKLTIKKISLILQSIFVIEVKQHRGVTGLPYK
                     YYKNILPNPVTDKKVIRVYTIKKHIDIEDIKKELGLIDNDLSIEHLVEARINKLLNAI
                     DETEKEVLLEGLI"
     gene            complement(10459..10761)
                     /locus_tag="ACOIAF_003007"
     CDS             complement(10459..10761)
                     /locus_tag="ACOIAF_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011572949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670008.1"
                     /translation="MLKDDLLRIGFDTYGVNLEDKLLENNEIYLNDKKESERIMNRLL
                     EILEEKEHKKMLIDFEEKVFGNSVIEKEYAYRMGVLDGLDLAEFIDLNKKLCLKRN"
     gene            10927..11133
                     /locus_tag="ACOIAF_003008"
     CDS             10927..11133
                     /locus_tag="ACOIAF_003008"
                     /inference="COORDINATES: protein motif:HMM:NF024835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGZ2670009.1"
                     /translation="MLDIQINKLLENQNKTRYWLAKQIGMTHQNLTKLANNNTNSIKF
                     DSLEKICMALKCSPNELFGWEQDK"
     gene            11213..11758
                     /locus_tag="ACOIAF_003009"
     CDS             11213..11758
                     /locus_tag="ACOIAF_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011572951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670010.1"
                     /translation="MDRYTFDNLPIKEQIQYINNKLLEGNTLTNTCKDIGIGRTTIRN
                     RFKQHGYEFNPTKKLYISIIEVVELESSSKVVAGDTSNDNTLVDATNIDFNNILSKFN
                     DMNNKLNEVYSWYESQSSNKVVGADKLNIDDFKGNIVVRSYKVYEDIQKEFVAFCEAN
                     KKYRVQDIISQALRDFMDKYK"
BASE COUNT         4134 a         1982 c         1342 g         5068 t
ORIGIN      
        1 aaaaaaaaat aaccatacgc attgatttta cttaaattaa aagtgttttt cgagttgttt
       61 tttcacttag tttagtaatt ttgaaatatt ataatattaa gcttcaatat gtatctatta
      121 tcatttaata atcaattaat aatagatatt ttttctaaac aagataaaaa caatatctat
      181 accagtaata gcaatagata gaagtatata aaaaaatgta cgattactat aaactattac
      241 atttatttta gttgccttga tacattgcaa ttacttacac atacgcctat ttaattaatc
      301 attaactata aaatgtaaaa acagcaaaga aaatcataca tttttaacta taaattaagc
      361 ttcataatgg cattatcagc ttgtttttgt actaccccaa tataagttaa tgtatctatt
      421 tgattagcat ggtttaaagc tctttgtgct acttcaattc ctttatcctt aaatatatgt
      481 aaaccatgtg ttttcctcat actatgagta cctatattta attttattct acaagctttt
      541 gaagcttttt taataattct atatgcttgt gtagtgctta ttgcttcatt ctctcccttt
      601 tgacttttaa ataaatattc attagttggt ttatctttgc aatatatatc taatatttcc
      661 ttcaaactat catttatagg ctttcttatt cttttccctg tcttactttc ttttatattt
      721 atctctcttt tcttgtatac gtctttaact cttagtttta gaatatcctc tatcctaaag
      781 ccagtattta accctaaagt tattagggtt aaatctctta ttttaccttt atttctaaaa
      841 tatcttttaa gcttctctat atctttttta ttttcaatgg gctctactct catttaaacc
      901 actcctattt ataaatccca aaatggatgt tcataaaaaa cacaatatac acttttccct
      961 ttgaattgta tttgccaagt aagatgcttt tcttctctta tacttttagt ttcatctaaa
     1021 cattcatgta aaagttgttc atcagcttct tccactctaa aaacgcccga tttattcttt
     1081 tcaaatacat ctgctaattt tacttttctt aaatctaatt ttggtatttt tctataactt
     1141 cttttagtcc cttccataaa aatcctcctt agcttttcct tcatttttta aatgatatag
     1201 ttccattaat tcttttggat tcatttcttc aatttctgtt tttgaaattt tatgaacatc
     1261 tattaaattt gttttataat attcctctaa catttcagct ccctgtgtaa aaaatatctc
     1321 atgttcaaca tctttcattt taacaatatt gttgttatcg ttcttattgt taatattttc
     1381 tttattttta aatctttcat tttgaagccc tcttaaaagt aaaaccgata taaaatcatt
     1441 tactgtttca tattcaaatt cttctgacaa agtttctata tcatcaagaa tatttttatc
     1501 tatatcaata atttcactat ctaaataata gttttttaaa aaattatcat atctttgttg
     1561 atacaagtgt actagctcaa catccctttt agtattctca acatttcctg ctatattttc
     1621 attatcaata tgataccaaa cattaatata tttatttttc ttaaatccca tttgttttaa
     1681 aatatcatat acacttgacc ttgatttata cagtttttct gcaactgtct tgaccttctc
     1741 attttccaat agatgtaata ttttttcagc ttcttcagac ctttttataa gtattaatat
     1801 ttcttttaca atttcaaatt ttaatttcaa ttcttctgtt gttaaagctc cttccttatg
     1861 taattcactc atttcaccaa taaatttccc aacctccatt gatgaatgtt caaacctctg
     1921 taagcattta tttaaagtgt atggatgaca gcctttttcc tctgcaatat catataaaaa
     1981 cacattttct tctgttatct gcttagttat ttcagttagt tcttcattag taaataaatc
     2041 atttcttaaa taaaatctta aatcaattat attaatcact cctttttaat tatttttcat
     2101 acattaatct tatcaccaaa aaaattaaat ttaaatattc caagacatca tctggacgct
     2161 aaaaagtgtc gtttttatta atttaataat attatcaaaa aaaagagcaa agacagtccc
     2221 ctaaaataaa aaaagaacta gttataaccc tagttcttct ttgctctatt gcttcttttt
     2281 atttctccac cttcatatac ctttatccac tcgtaaagcg tagttctacc aacatttaat
     2341 attttcgcca cttcattctt ctttaaactt ccatcaagaa ttttattata atattttata
     2401 aattctttgt tggtttctaa aagctccttc gttacttttt gtctaccgaa ccattttcca
     2461 ctctttgtat ttatctcgcc agtatttttc ttatcctctt tcaactggtc tattttctgc
     2521 tgtgttctct cttgtattgt ttccctctcc atctctgcaa cagctccaaa tataccaagt
     2581 aataacttat aagtattgtt tcctgtgtct ataccttctt ttaatatttt tactcttaca
     2641 cctttttgaa ccaatgtatc tataatatca attaaatcct ttaaatttct acctagacga
     2701 cttatagact cacaataaac cgtgtccccc tcatttactt ctaacaacat tctatttaat
     2761 gcttctcttt cttttgtagc tcctgtgact ttatccctat atatcttgtc aaacttaata
     2821 ccctcatttt ctaaaactct ttcttgtctg ctattatctt gttttcttgt acttaccctt
     2881 tcatatccta ttatcataac actacccccg tttatcttgt tataataatt atacaatata
     2941 ttttccgttc ggtcaaacta tattttctat tttcacaata ccgaacgtat tttattgttg
     3001 atttccaatg attctatgta aaaataacat aaatatcata cgtacggtta aacgttggtt
     3061 tttccgaaca cgaacatatc acctttttta tatcaactat tccttaaata ttttatataa
     3121 aatcgccaaa atctttttat attttttagg caaccaagtt tttaaaacaa aaatggtata
     3181 tcagtaagaa ttatgttttc acacaatata attcttaatg atataccatt taagaacctc
     3241 ataagaggta gatagagata attacataat catcacatac gttttatcaa atctgttttg
     3301 taatagggtt tatacttaaa taataacaaa aataccgtat tttttctaca tttatacttt
     3361 ttttgttgat agtaaaatat ggaaattagt catattttca gttattaact cataagttta
     3421 tacaactttt atttaaatat aataaaaaca tcaataataa ttaaattatt gaagcatata
     3481 tttatattaa tatctatcat ttaaatttga ttgaaacagt tttatatctc taatggattt
     3541 tttagaagct tttacaatat cagttataaa tcttatttca tactcattac aatccctcaa
     3601 aacttcttct aactcatttt caaagacagc tttagaactt aagattgtat cacataataa
     3661 ttcatcaacc gacaccccaa gagtatttgc aatagcaacg agcgttggca aacttacact
     3721 tgaatttccc gtttcaatat tgctgacatg ttgtggacta acattgattt tttctgataa
     3781 aacttcttgt gttagctttt tcttgattct tgcaattttt atcctttgac ctataaattt
     3841 gaaatcaagc tccatatgcg tattcccttc ctacaagtat tcacacatat tgtaaccttt
     3901 cccaaagtat attataataa attaatagaa tgtttcatta tgatagttat agtgtataga
     3961 aaggaattat atggatatac caaagatacc tcaaacagaa ctcaaagtca tgaaatttat
     4021 ttggagcaaa aacgacattg taacatctaa agaagttgca aaagctatgg aacttgaata
     4081 tagttggaaa atgacaacaa ccctaacaat cttatcaagg cttgttatta aacacttttt
     4141 agcatctgaa cgaataggca gaatgacaca ttatacgata ctcatagcaa aaaaagatta
     4201 taaaatatct gaaacaaaaa gatttctaaa tgacatacat tcaaattcat tggaaagcct
     4261 tttaaactct ttgaaagatt gcaatattac acataaaaaa tagtacatga gtataaatta
     4321 tattattata cctatgtact atttaagttt ttctataatg ttcctatatc tatctaattt
     4381 tgattgattt agatacttct gtagtagttt tcaaaacgtt tcttaactct tgtaagtgta
     4441 ttttcttttt ttaatatttt tgagatgtac ttattaatgt ttttgactca ttttcagcag
     4501 taattattgc aacagctcta taagtatgtc cttttgtcac agaatatgag tttaaaacag
     4561 aacaagtttt tcctccagtt ttctttgtag atgtttttaa atttacccaa cttccgcctt
     4621 ttaattcttg taacttagtt acaatagatg ttctagttac tccagatttt ccaacaacag
     4681 aagcatttac atttgctttt ccattagaaa atgataaatt acatctacca tttaatatgt
     4741 aaactgaaaa aggatttatt tcttctttac catcttccat aaaaataata tcagatgaat
     4801 taaactgttc agcaaaacta acaacattta ctgacattat tattatcaac gcacatacaa
     4861 atattgagat tatatttttt actctcatta agtacctcct tataaaatct tatatattca
     4921 atataacaac taactagcac tattttagac aaaaatttac ttaatatttt ttgcaatttt
     4981 aataaattct tttttatctt tcttagttat tttttttgta ggtagctctt tagataacat
     5041 ataaatatta tttttatcat gccacaataa ttgttgagta tttccttttt caacatactg
     5101 tgcatactga ttattaaatt ttatattctc aacaaaagca tcctctgtat ccaagactat
     5161 aacatcccta gaatataaca tcatttgata aattatatag ttatctaatt tatctttata
     5221 taatattgat agataatttt cttcctcaat tctctcaact tctttatatc cagtagaaat
     5281 gtatttaggt tctatatatt taaatgatac atttgattta tgagggttta taaaattata
     5341 gtctgtaaat tctttatata ccttcttcat tattctaatt aaattttctg gaaaagcttc
     5401 tgtttttata gtagttatag tgactatact tgataaaatt attataaaaa caatagcaat
     5461 tttttttaag tttttatagg tgttcttttt tacttttttc tcattttctt ttataatttt
     5521 atccatttta ctttcaaatt ctatagaaaa ttcatagtca atatcttcat cacaaggtaa
     5581 ttcatacagt attttttctt ctatttttgg aagctgttcg tataataatt tttctatgtc
     5641 taatttatct ttattcattt gataatgcct ccatttcgct taaaattttt tttaattttt
     5701 ctaatgctct tttatttctc ttatacaaat taacctcttt tatattcagt agtttagaaa
     5761 tttctctgat agaaaaacca tgactatatt ttaagagaat cacctgtaaa tctgtctctg
     5821 acagtttttt taaagcttct cctaaataac tacactcacc taacttttca atcgataaat
     5881 ctaaagagct gaatttatag ttatcatcat taatattact tatttcctct ttttttcttc
     5941 tattatatat atttattgaa atccttttta ctataataac aacaaaacct ttagtttttg
     6001 gactattcac atcatttact ttatcaagat tatcaattat ttttaaaaat gaactatgaa
     6061 cagcatcctc cgctaaaaca tcatctttta aaatttgatt agctacataa aacattaatt
     6121 tcttatattt aatatatatt tcttcaaatt taattctttc ttcttttgaa tctataatag
     6181 ctaaatatat taacataaat tattctccct taaaaaatat tatccttaaa aaaattatat
     6241 acaatttata aaaaattata tataaatgtc taaaaataaa aacttcattt gttatatata
     6301 taaataaaaa cataaaagga tggtgtttta tatgaaatca aaaagaataa acaaaatgtt
     6361 agcaactaca atgacaatgg caatgctagc aataccagca gtaacagcaa catcatatgc
     6421 aatggaatca acagggttag gaacagaata tacatataat aatgaatcta aacagcaaga
     6481 agagtgggtt aaatcattcg atactgaaac aggaaaagaa atattattaa ataaaactac
     6541 taacgaaaaa gtttctgaag tatatgaaaa tgtaaatgga aaattggtta aattaagtat
     6601 taatgaagca ctagaaaaat ttaatttaaa tgaggaaatt atcacaaaat ctcctaagtc
     6661 tatttttgga gtatcaagtg ttaaaaaaat tactggttct gcacgcaaaa tatcacaaga
     6721 cgtatcaggt ggaaaacaag gtgctactat atctatagga aattcaacaa ctgtaactga
     6781 aagttttact gttggtggaa ctacagaagc aataaaggca gcagtaagag caaatgcagg
     6841 atttacatgg agcaaatctt taacaagaag tgtcacaact acacacaaag tacctgcagg
     6901 taaaatagga tatgtagcat ttaaaccata ttataaagaa gtaagaggta cagtaaatgg
     6961 gatacttgca ggaacttcta taactcaaaa aatagtagca aaaagtcctc ttaaagcagg
     7021 aaaagtttgt gatgggttag aatatcttgc atacaaataa taaataatag aaagagttga
     7081 ttaaaatgga aaataaaaat atgtacttaa gggttagttt aatattagca gtattaggct
     7141 ttataatatt attttttaat aatgacttag cattatcact aaaagcaaca tacttagctg
     7201 ataaaggctt cgaagacata gttgaaaatc aaatattaaa aaactattcc tatatgtttt
     7261 taataatagg tggtgtacta ttttccatag gaatctataa cttaaccaaa ctgaaacaaa
     7321 ttaataaaaa ataaataaaa taagaggact tataaattat aagtcctttt attttacact
     7381 tataagctac tttcaacctt tcatgattta acctttattt atacaacacc caaaactcga
     7441 cctcaaaaag agaacaggac ttcgtccccc ttaatacaaa gcaacatttt ttagtactat
     7501 tcgtacttaa gattagctat tagattaatc cttccaaaag aacctctttt tccgtttcat
     7561 caatcgcatt taaaagttta tttattctag cttcaactaa atgctctatg cttaaatcat
     7621 tgtctattag tcctaattct ttttttatgt cctctatgtc tatatgtttc ttaatagtgt
     7681 aaactctaat tactttttta tctgtcacag ggtttggaag tatattttta tagtatttat
     7741 agggcaaccc tgtgacaccc ctgtgttgtt tgacttcaat aacaaaaatt gattgtaata
     7801 ttagacttat tttcttaata gttagctttt tcttagttgc taaaaacgtc tgtaaatcaa
     7861 cggctagagc ttctaagtgt tcttttgtat aacttgtacc aactccaaaa ttgaagttta
     7921 tataattgta agtataagta ccaatattat catcatttag caaagaattt gcgtattttt
     7981 tattaatttt tcgataaatt gttgtattta tctgttcaaa aaatagattt cttttctcgt
     8041 ctgacattgt cgcaagtttc caagataggt caatactata actattatct aatacaaaat
     8101 cactaaataa gtctatcgtt tttctacaat tattattaat tattaggcta tataaatcat
     8161 gttccatata atattttaaa cagatattat tatctatcct catgtcatga tgatatttta
     8221 ataagttact gctggtcatt gacttcttaa tttcactata gccaactaga tactttttat
     8281 gtccttctag ttgttctata gctgatttct taaattcctt aatagcttcc tcatgctctt
     8341 ttatttcttc ctctattttt ttagtttctt tttcagtttc tactatctca attttattaa
     8401 aatattcctc taatagcact ttgaagctgt ttattgttct tgtttggtaa tagttattgt
     8461 aagcattact ttttatgtat aacttgtccg ttttatagca catatctttt ttatcatagt
     8521 atatattact atcaatgttt tttatgttta tagggcttat atcaattcct atagctctat
     8581 atggtaagtc tctcttagaa gctttattgt atatatttgt taggctctca acgtctttta
     8641 tgttctcatt tatgcaccat tcaaccgaat atacattgct atcctcattt gtcgttttgt
     8701 aactattaaa aatatgtaca tttatacttt ttgcccctct agctcttgca acatactgtt
     8761 ttatttttcc tacatccttt tctccaatta caataatatc tgttacatta ggattattta
     8821 tgtttacacc tgccgtgatt acagacgtat ttaaaagcac ctcatagcct tccatatcgg
     8881 agtaactaac aattctatca tatagtttgc tagtgtcttt agtatctgaa ataacaactc
     8941 cactcttttt attaactttc tttgatatat agttaagtgt tgatatacta tcttttaaaa
     9001 gcattgaatt atttgaatta tttagtatct ctattacttt cccaatattg attttatcgt
     9061 ataacttaac tttgtattcc gtttgtatat tttgtttata ttcaataact ttgttatata
     9121 tctcaaattc taatttagag ggtgttgcag ttatatctag tctacctctg cacttattag
     9181 ttatattatt taagctcttt attgctttgc ttctgaacgc atctgtgtaa gtctgatgta
     9241 tttcatcaat aactattatg tactcgctca aatcctcttt tagaagctgt tcagtcttat
     9301 cccacgtcat agccactaac ttgtttccct ctaaagcgtg tttagtaggt atcttatcat
     9361 atgcaccagc tattttatat tcgactattg cttgttgcac attcgcagag ttggggaata
     9421 taaagagtgc ttttatatcc agctctttta atgtatttat aattgagtat gtttttccac
     9481 ttcctgtagg agctaccatt aatacgttct taccttctgt agcatgttta attgactctc
     9541 taataccttt tagattttca cttatgtatt tatctatatt aattatcgaa tcagctttat
     9601 aacttgaata gttttgtttt tctagcatgg attttatttt aatattgtta tctaagtaat
     9661 actttctttt gtccttatca gtcatacact caaattcaaa agctaactca atatcattgt
     9721 ttttaaaagc ttcttgtttc ttttcttcta tgaagctatt tatatctttt ttctttatgt
     9781 aaatgttttt aatagtatta tttgtttttt taatatttgt cttactcttg ttaattggta
     9841 atgagatatt atatttattg gctaggaata aaatagcttc tataggtctt tcaaagcgtt
     9901 catatgtgcg acataattca attatatcag caccaacaac acaattatgg ctatagcatg
     9961 tatatattct tttgtttaaa tcgaattgga agtttttatc cttaccacca tgaattgggc
    10021 aaggaacatt ccttttttga gttgtatcga ctcctatatc tcttaaatat agttcatcta
    10081 tattggcatt ttttattata tcaattaatt gttttatatc tattttcatt tgtaaaaatc
    10141 cccctttttt agttaggggc taatcaagtt ttatcaagct atgtatttac attctgccgt
    10201 aaatctcgta taattaacat ataaaagaat aaggaaatat atattttata tgttaattaa
    10261 gcctaagtga ttagctccta gtattaaccc ctagtggcta actcctaatc ttgattaacc
    10321 cttaaatgga aagaatgagt ggatgccgtc aaacaaatac tcgttctttt tcgttttttt
    10381 tgttattagt attatatcac tattaaagtt ataattcatt gtttagaatt atttttctga
    10441 cggttttaat atatattttt aatttctttt caagcataat tttttattta agtctataaa
    10501 ttcagctagg tcaagcccat ctaaaacccc cattcggtat gcgtattctt tttcaattac
    10561 actgttacca aatacttttt cctcaaaatc aattagcatt tttttatgtt ctttttcttc
    10621 caatatttct aacaatctgt tcattattct ttcagattct tttttgtcat ttaaatatat
    10681 ttcgttattt tctagtagtt tatcttctaa atttactccg tatgtgtcaa atccaattct
    10741 taataaatca tcttttaaca ttacttttaa cactcctttt tatgcacact taaatctttt
    10801 gcacccctaa atatggtaat atattctatt tattattatg taattattta ctatgtattg
    10861 tcaagcatat atattgtttt tggtacatat ttccgatact atataataaa aaaggtggcg
    10921 attaaattgt tggatataca aataaataaa ttattggaaa atcaaaataa aacacgttat
    10981 tggttagcaa agcaaattgg aatgactcat caaaacttaa ctaaactagc caacaataat
    11041 acaaattcta ttaaatttga ttctttagaa aaaatttgta tggctctaaa atgttctcct
    11101 aacgagctgt tcggttggga gcaagacaaa taaaaaaata taaatcctaa agggtttatt
    11161 tataactact agaaaactac tttagtagtt attttatttt ggaggtttaa acatggatag
    11221 atatacattt gataacttac ctatcaagga gcaaatacaa tatattaata acaagctctt
    11281 agagggcaat acattaacta acacatgtaa agatattggt attggtcgta caactataag
    11341 aaatagattt aaacaacatg gatatgaatt taatccaact aagaagctat atatatctat
    11401 tattgaggtt gtcgaattag aaagtagttc aaaagtagtt gcgggcgaca ctagcaatga
    11461 caatacttta gttgatgcaa ccaatattga ttttaataac attttatcta aattcaatga
    11521 tatgaataat aaactaaatg aagtatatag ttggtacgaa tcacaaagta gtaataaagt
    11581 agttggggct gataaactaa atatagacga ttttaaaggc aatatagttg ttagaagcta
    11641 taaggtctat gaggatattc aaaaagagtt tgtagccttc tgtgaagcta ataagaagta
    11701 cagggtacaa gatattatca gccaagctct tagggatttt atggataaat ataagtagtt
    11761 ttacttactt tctgatattc taaaaacttt tcaatgaaaa tctcattttt tggcttcctg
    11821 tgaaaaatat gtactccttc cagtcctcta tctttattta ctttattaat ccgatagggg
    11881 gcgtattata tatatttcta tcgagcatat actcatcaaa acatagcatt agtttgatta
    11941 ttatatttct atttatttta agattatata tacaatttgc cccttaatac attgcaatta
    12001 ctaacttctt tagttttgat attaatattt aaatcattcc tttttaaagg tgctataagt
    12061 gctatatagg cattgcaacc tatatacttt ttaggggtgc agttgcatat gcaagctctt
    12121 tatttcccaa cacttaacca gttataaata tttatatcct ttattagcca aatatagcat
    12181 tttaaacaac tccgactatc tttagtagta acattttaca tagctttatt aatgtatttt
    12241 tgcatctgca accatgtttt taagttgcaa caccccaccc ccacacctta cacaaaacaa
    12301 tacttatctc cgaagccata tacctacctc ggttcacata tttttcttca acagcgttat
    12361 tttcagcaag gggggctaaa cattatactt tttttcagtg cattaaatag ccttacatgt
    12421 attggtataa ctttatttca aacccttaat tgaattgttt caatctaaat taagtaaatt
    12481 ttaataagat aaaatatata gtttgtaact ttatttcaaa atacag
//
LOCUS       DBGJFF010000115        12410 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.117, whole genome shotgun sequence.
ACCESSION   DBGJFF010000115 DBGJFF010000000
VERSION     DBGJFF010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12410
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.117"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(86..706)
                     /locus_tag="ACOIAF_003010"
     CDS             complement(86..706)
                     /locus_tag="ACOIAF_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HGZ2670011.1"
                     /translation="MQYIQELLDLTQNYWVLATIIGLLSAFIESFIPALPLVAIVTAN
                     AAIQGLFIGCLLSWIGSGLGTTSLFLLISRFNDSKLFNKLRNSKTEKAISWMDKQGFK
                     LLFIAYACPFMPGCLVTIASAFGKKDLKDFVPAMLAGKFVMFIVISYVASDIEGFITS
                     PLKIASFILLVFLSWKIGNRVNKNLENHNYDFHHKKHHNDKDDKMI"
     gene            complement(1020..3158)
                     /gene="vanT"
                     /locus_tag="ACOIAF_003011"
     CDS             complement(1020..3158)
                     /gene="vanT"
                     /locus_tag="ACOIAF_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HGZ2670012.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKKLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKPLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVKMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSSPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINGCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLSE
                     LSASEVANNSCSISNELLSRVGRRLNVIKK"
     gene            complement(3176..3982)
                     /locus_tag="ACOIAF_003012"
     CDS             complement(3176..3982)
                     /locus_tag="ACOIAF_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HGZ2670013.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            complement(3979..5079)
                     /gene="vanG"
                     /locus_tag="ACOIAF_003013"
     CDS             complement(3979..5079)
                     /gene="vanG"
                     /locus_tag="ACOIAF_003013"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HGZ2670014.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNNDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            complement(5340..6422)
                     /gene="vanS"
                     /locus_tag="ACOIAF_003014"
     CDS             complement(5340..6422)
                     /gene="vanS"
                     /locus_tag="ACOIAF_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS"
                     /protein_id="HGZ2670015.1"
                     /translation="MRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKA
                     ALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEV
                     ALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
                     LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLE
                     QLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEIT
                     AVQEDDYIKLKFMNHSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVEL
                     HNGTITAHSENNIITFKVIIPIVGNL"
     gene            complement(6472..7173)
                     /locus_tag="ACOIAF_003015"
     CDS             complement(6472..7173)
                     /locus_tag="ACOIAF_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-G-Cd"
                     /protein_id="HGZ2670016.1"
                     /translation="MNENILIVDDEKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KNLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKTQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            complement(7607..10138)
                     /locus_tag="ACOIAF_003016"
     CDS             complement(7607..10138)
                     /locus_tag="ACOIAF_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HGZ2670017.1"
                     /translation="MSLKSLKIKENLYWVGSLDPDLRVFDIIMYTPYGTTYNSYVLKG
                     TEKTVLFETVKDKHFDNYIERLNDLNIDFEKIDYIVVSHTEPDHAGSVEKLLDLAKNA
                     KVVASETAIKYLKEIVNKDFEYVAVTDGDTLSIGDKTLEFFSVPMLHWPDTIYTYIKE
                     DKTLVTCDSFGSHYSNDKIVNTLDENEEKDYLDALRYYYDCIMGPFKPSMVTAIEKIK
                     DLDIDTVCPGHGPVLTENPRKIIDLYYNWSVNEQIKLEKEVTICYVSAHGYTKIMAEA
                     IKAYIEKNSNYKVNLFDVIEHKQEDILAKIAVSQGVLFGTPTILGDALKPIWDILISL
                     NPVLHGGKVASVFGSYGWSGEGIENAMERISQLRMTAVKPFAVNFKPSNEEIDKLHSY
                     TGKFLDKLNSTFGSKKKTKKFKCVICNEVFEGDSAPSVCPVCGAKEDQFIEVEEDEVT
                     FRKDTDEYFVIVGNGAAGFYAADAIRKRNKTCKITMISNEDELTYYRPALSDGINEEL
                     GSDFYMEDKDWYDKNNIVVILGTNVDKLDEVNKTIIVNDGAIKFDKLVIATGSRNFIP
                     PIKGHDLENVFTLRNIKDLYSVKEALEKSKKVVVIGGGLLGLEAAWEFRLKGLEVVVV
                     EAMDSILSKQLDKEGSKILEQCVRDTGIDVRLGVAVDGIEGDGKAQKVVFKDGDSVDC
                     DMVVFSIGVRANTQMVQDTSVKIDRGIVVDKTLQTNVKDIYACGDVAQVGNISLAIWP
                     SSVEMGKIAGANASGDNLTFESEVYPVSLDAMNVKVFSIGNIQNFDKEISSKDEGQRI
                     YKKLFMKDGSLVGAILINDLSCTVKLIRLISEKGDFEDIMKADIL"
     gene            complement(10246..10863)
                     /locus_tag="ACOIAF_003017"
     CDS             complement(10246..10863)
                     /locus_tag="ACOIAF_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HGZ2670018.1"
                     /translation="MCKKTYLLVVSMLIAGLLTACSGSPTKDSGQAQDNTKKEANASN
                     NALKDEKNNENLMEQDFKVPYTDAINIFKDKYKDADIVDLSLERDLNKFVYTVEGVDD
                     NNEYKMKIDANTKDVLEDKTEKLDSEDLNGVARKEKLDLNDIMTPQQAMEIALKEQNG
                     IVKEWSLDKDLDVTFYKIRIDKDKNEYDIKVDSKKGTVLKVEKED"
     gene            complement(11225..12109)
                     /locus_tag="ACOIAF_003018"
     CDS             complement(11225..12109)
                     /locus_tag="ACOIAF_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HGZ2670019.1"
                     /translation="MDTTKIDDISKLDLNNLDINKMDMGGIMESLLEWATTSGVKLII
                     GILILSIGFKIIKKFVNHVMLLLSKRDIDLTLRKFLKSLLLSVLKVAVIIIVLEYWGM
                     SLSSFAAVIASAGVAIGLALQGSLSNFAGGFIILLIRPFKVGDYVEAAGHGGTVEQIG
                     LFYTQLVTPDNKQILIPNGSVSNDSLINYSAKNTRRVDLTFSVGYEDDILHVRRVLKD
                     IVNNHKLIINEPEPFIGVVEHGDNAIKFATRVWCKTEDYWTIYLDLLEEVKVKFDEEG
                     ITIPYPKMDLTVKELNKI"
BASE COUNT         4212 a         2222 c         1274 g         4702 t
ORIGIN      
        1 ttttgtactt taatacaaaa aataaaacgt atcttaaaat aatttagtac taaatcattt
       61 taagatacgt tttatttcgt acaagctaaa tcattttatc atctttatca ttgtgatgtt
      121 ttttatgatg gaaatcataa ttatgatttt caagattttt atttactcta tttccaatct
      181 tccatgacaa aaacaccaat aatataaatg atgctatctt taaaggactt gttataaaac
      241 cttctatatc acttgccacg taacttataa ctataaacat tacaaatttt cctgccaaca
      301 ttgctggcac aaaatctttt aaatcttttt taccaaaagc tgatgcgata gtaactaaac
      361 aaccgggcat aaatggacag gcatacgcta taaataataa cttaaatccc tgtttatcca
      421 tccaacttat tgctttttca gttttactat ttctcaactt attaaaaagc ttactatcat
      481 taaatctgct tattaataag aataatgaag ttgtcccaag tcctgagcca atccatgata
      541 acaaacatcc aataaataat ccttgtattg cagcatttgc tgtaactata gcaacaagtg
      601 gtaaggctgg tataaagctt tctataaatg cacttaataa gccgattatt gtggctaaca
      661 cccaataatt ttgagtcaaa tcaagtagtt cttgtatata ctgcatattg cttttcccct
      721 tttttaactt taactattat tatatactct atatgtttat tatataccaa tctcaaaaaa
      781 tgaatctaat attaaaaagt aaataatcta tataagttta cagttaaggt ataattattt
      841 tattatatat tataacaaaa aaggtgatgt ttcaaaataa aaatctgaaa catcaccttt
      901 ttatagctta aattgtcatg taagttataa aaaatatata ttagacataa tattactaaa
      961 aatgtctagt cagagtaaaa taaattacac agtacaacaa atattactat ttaattgagt
     1021 tacttcttta taacatttaa cctccttcct actctgctca ataattcatt tgatatacta
     1081 catgagttat ttgcaacttc agaagcagat aattcagata aattatctct tcctattatt
     1141 attgccgtat taccaacttc tacattaggt atatctgtaa tatcaacagc aagttggtcc
     1201 atgcaaattc gacccactat tgctgctcta caaccattta ttattacgta actttttcca
     1261 caggataaat ttctaggtat accatcggca tagccaatag ataatatagc aattacgcta
     1321 tctctgcttg caataaagac tcttccatat ccaaaactat catctttttg tatttttcta
     1381 ataagaatta cttgtgattt taaggataaa acaggtcgta aatcaagttg caatttagtc
     1441 gtatcatttg gagaacttaa tatcccgtat aatgcaattc caattcttac ataattacac
     1501 ttaagattgg gataattaaa aaaaccataa ctactttgaa tatgtacttt aggaattttt
     1561 ataccttttt ttaacagaaa atcaattaac ttataaaaac attcaatttg tttatttgta
     1621 aagtctacat cttcatcttt cagactatct gaaacacaca agtgtgtaaa tatgccacat
     1681 atatctaagt attttaattt aaatgccttt aaaattgacc tcacatcatt aacatcaaaa
     1741 cccagtctgt gcatacctgt atcaatttta atgtgtgatt ttatgttgta tttctgtttg
     1801 tttaaagaga tagcatgatt aaggtcaata acactctgtg taagattgta tttatgaagt
     1861 tcctttgccc ttattgggtt agtatatcca agaatcaata tttctcctag tattccatat
     1921 cgacgtaaat caatcccctc atctattgtt gctactgcaa atgcttttac acctatttgg
     1981 tttgcacaag tcgctacttc aaaagcacta tgaccatagg cattagcttt tacaactgcc
     2041 atcaattcac aatcttctgg catagctttt tgtatcatct ttacattatg ttccaaatta
     2101 tttaaatcaa tttcaatcca agctctattg gtatttacct tatgttctcc tttcaagtct
     2161 ttttttagag gtttaagaat caatataata attatagaag ctataaaaga tgctatactt
     2221 accactatat aatgaataag gctattgtca accaagacag tttgtattcc gaatatttta
     2281 gaaaacatac gaataacaac aatcatcata ggatgtataa tataaataat taatgatgat
     2341 gttcgcaata acctagctcg agaacctttc aaaaatgtta aggctgtaaa taagaaatac
     2401 atacatggca ataacataat atacatgcta tcatgacgct gtacccctag tttatgtaaa
     2461 aacattccct cacataacat aagtaagaat gatataagaa atccaatcaa gctattttta
     2521 gttgacatat aattagattt attagatatt attccaccaa gtatgaaaaa tacaggtgca
     2581 aaaaatatac catttcgtgt ataatctgat acttcaaaaa tatacccata taaattcttt
     2641 aatagtggta ttttttctaa aaaaccataa taactatcac caaacatacc aattacataa
     2701 ataacaatag tgattactag tgagtacttt aaacctagtt tttttacaag aaagtatgca
     2761 atacttgcac caataattga tgcaggaaga taccataaat gatataaagt cccatcaaat
     2821 acaatgtcct taataatatt gggcactaaa ttattcatgg aaaaatatcc agtgtaaata
     2881 tttaagggaa tataaattac aatagctatc ccgtaaataa ataatacatt ttttagaaaa
     2941 gatttcagtt tattacaatt ataattatat gttgaaatta gaaaaaagcc tgatagcata
     3001 aaaaagaatg gcacagcaat acgagcaatt atactagtta acacaaagtc agccatttta
     3061 ctatatgata ataatggaga tgtatgaatt gcaattataa gtaatgcagc tataatccta
     3121 aaataatcaa gccctgcata agatttattc ctaatcattc ttgttcctcc atttcttagt
     3181 cctttctatt tctccaaaat gtcacaataa aaccatcctt attgttaccc gatatttgat
     3241 atacacattg ttcaggtata gagattctca cttcttcttt agtatgaata ggcacataaa
     3301 agatttctat aattttttct ttatcttcta tttttaaaca atttgtatac gaaaatctct
     3361 taatatattc tatatattct tcaagagcga gattcatttt attgataatc tcagaatgcg
     3421 gatatcccac ataacgaaaa tgccatggtt cttgagatat tcctgttatt tcttcttttt
     3481 tacactgata acgctctata aaaccataat gtagtgctgc cttacgaaac tcattacaaa
     3541 taccattata aggaaattca gggcagataa aatctataac ttctttcttt aaccctaaat
     3601 caatagcaag ccctgtctga tgttcactgt gatttggaag tgctacaaac tttttagtaa
     3661 agtcttttcc attgtcacgc agagaacttg aatatatatc ttgttgttcc tctgcagtac
     3721 gatatccact tacaggtact atttcattta ttccacatat actttttaaa atattttgta
     3781 aaatgtttgt agcttcacgt ttcataagaa tacttggaaa ccttgtatct acaggaatta
     3841 aatctatatc ttcagatgtt ttaactggta aaaaagcatt tactagaatt agattcccat
     3901 cataaacttt atctttagtc aataagatag tatttgtcat tttatcttct ttatttttat
     3961 ctaataaaat attattcatc attttatgta cacctcaatt atcttgtcca atatttcttc
     4021 aaaagacagt ccaatacctt tcatcatatt tggataacga ctatgagaag taaagccagg
     4081 aattgtattg acttcattaa aaataatttc accagatgga gttaaaaaca tatctactct
     4141 agcaaatcca gaacatccaa gagttctata aataattttt gctgtctctt gtattctttt
     4201 ctcacaatcc aaatcaattc tagctggcat atgaattttt gaacttttca atgtatattt
     4261 ttctgtataa tcaaaaaatc cttttgacaa ttctatttca tctactcttc caacaagtaa
     4321 gtcattgttt cctaaaactg cacaccctac ctcaaaacca tcaatatttt cctcaattat
     4381 tacttcatta tcatgttcaa aagccaagtc tactgctact tgtaactctt cttttttata
     4441 aattttacta ataccaaatg atgaacctgc acgcacaggt tttataaata atggataagt
     4501 taagtctttt gacagttcag ccacattaaa tttatctgag tattttagta atattgattt
     4561 tggtacttta ataccttctg aatgaacaag tttatgtgat ttgtctttgt ccatacataa
     4621 tgctgaagaa agagtaccac atccaataac ctgtatacct gccaattcaa ataacccctg
     4681 cactgttcca tcttctccat tttttccatg cagtacagga aacacaaaat caagtttagt
     4741 gtatttaata tcattttcac atgattcaat agcactattt ggaatatgat tctgagaaac
     4801 agcatcagga attaaaaatt caataatacc atttttgaca cggttctgcg aaacaataac
     4861 tggaattata tcagtatccc tttcaaacca agtgttattc tggatattat tataatctcc
     4921 aatataatga taccaattac cctcccttgt aattccaatt ggtataatat catatttttc
     4981 cgtattcaaa ttctcaagta ctgcaaaagc tgattgtaat gatacatcat attctgttga
     5041 gtttccacca aatataactg caattttttt attattcatc tttgattctc ctttgttgat
     5101 ttatttattt ttatacaatc tacagaaaca tgttaaaata ctttttctat ttcaaatcca
     5161 atcaaccttc acttacaatt gcatctgttc cacataatat attttcacat ttttctattg
     5221 atataattac tgtatcaaaa taaaagcact cttgttttga tacagtaatt atatcaataa
     5281 caagagtgct ctttatttat ttatcctaaa gaatttccta tttttttata cttaaattct
     5341 tacaaatttc ctacaatcgg aataataacc ttaaaggtaa ttatattgtt ctcgctatga
     5401 gctgttattg tcccgttatg aagctccact atttctttag ctatagcaag tcccaaccca
     5461 gctctaccac tgtttgaatt acgagatgta tccaacctgt aaaattgttc aaaaatacgc
     5521 tcaagttttt cctctggaat cgtattgcta tgattcataa atttaagctt tatataatca
     5581 tcctcctgta cagctgttat ttcaatagta gtatcttcaa aactataata aacagcattt
     5641 cgtaataagt tatcaaatac acgttgcatt ttattaacat cacaccttat catcatattt
     5701 ggtgtaacat tcagtgtata ttttaaattc ttactcatca aagtaggttt aaactcatat
     5761 gttagttgtt caagcatacg agttaagctt actgtactgt attcaagtga tatatttgat
     5821 aaattaaacc ttgtaatttc aaagaattca ttaattaaat cttcaagacg ctctgcctta
     5881 tccaacgaaa tagataaata tttttttcgc agttcttcag aaatctgact ttcatctcga
     5941 agtagtgtca gataccctat aactgatgta agtggtgttt ttaaatcatg tgcaagataa
     6001 acaataagtt cattcttacg ttgttctgcc atttgtgtct caaatttacg ttgttcaagt
     6061 atatgtttaa ttgagtttat tttcttttct attgccaata actctggaga taaagccact
     6121 tctccaacat cttctttgat aagagagtca ataccttgat ttatttcact aaaatatttt
     6181 gtaaaccaat ttaaataaac acggaatatt ataaaaaaca caatcaccat agctagtatc
     6241 atgaagagct ccatgctatt acggaaaatc tgctgatata aattcaatgc agctttatat
     6301 tccatacgaa aagcattttc aaagaatgat actatccaga tagaaaactt accatacaat
     6361 ataaaagaat ataaaatcca tatgaaaata atggctataa atgccattaa agctgttctc
     6421 ataaaaattt tatttctcac ttgagtaaag tcaggattga ctttaatatt tttacttttc
     6481 aattgtataa cctactcccc atacagtttt tataaatttc gggtttttag aaggttcatg
     6541 aattttttca cgtaatcgcc ctatatgagc cataactgtg ttattattat caagatattt
     6601 ttctccccat acagcttcaa acaattcttc agaaggaacc acttttcctt gatgttcaca
     6661 taaataccaa agaatagaaa attcaattgg tgttaatgct acttctttcc caaataaact
     6721 acatttatgt gtttctttat tgataatcaa tcctttaata tcatactcat taacaattat
     6781 cgactgttgt tcataagaat tattatatcg catatatctt cttaattgtg tctttactct
     6841 tgcaacaact tccaaagggt taaatggctt tgttatataa tcatcagcac ctatggttaa
     6901 acccattatc ttgtctatat cttcaatttt agctgttaac ataatgatag gataatagta
     6961 tttttctcga attttctggc aaatatgaaa tccatcaata tcaggaagca taatatctaa
     7021 aatagccata tccaaatttt tagattcaat acattctaga gcttccttac ctgtataaaa
     7081 cttgtatact gtaaaacctt cattttttaa atatacttca attaaatctg ctatttcttt
     7141 ttcatcatcc acaatcaaaa tattttcgtt catagattat ttttcactct ccttattatc
     7201 gaggtcatca tttataatat tggtaaattt acatatcaaa taattacaag catatttgta
     7261 actaattttt ataagaaaag aactactaca tcagaatgtg ttgatgctac cattggaaca
     7321 caaaaactga gattaattgt tattataaaa actcccctaa cacgtcctta ttatcgcata
     7381 acaggtatta actttcaata aaaatatcta tcagtcaaac acaattcaat ttattatacc
     7441 agagacaatc catataaaaa ataattaaac ctatgtatgt aaccacagac attactaaaa
     7501 tttccatgtt attttatata aaaattcaaa aaaggagttg aatttaaaat ttgattagaa
     7561 aaaaatcaat tttaagacaa ctccttttta ttttaatgta tttaaattat agtatatcag
     7621 ccttcataat atcctcaaaa tcaccttttt cagatattaa tcttatcaat ttaacagtac
     7681 agcttaaatc atttataagt atagctccaa ctaatgaacc atctttcata aataattttt
     7741 tatatattct ttgaccttca tcttttgatg aaatttcttt atcaaaattt tgtatattac
     7801 caatactaaa tacttttaca ttcattgcat ctaacgaaac tggatatact tcactttcaa
     7861 atgtcaaatt atctccactt gcatttgccc ctgctatttt tcccatttct actgaagatg
     7921 gccatattgc taaactaata tttcccactt gtgcaacatc tccacaagca tatatatctt
     7981 taacatttgt ctgtaatgtc ttatctacaa caatacctct atcaattttt acagatgtat
     8041 cctgaaccat ttgagtgttt gctcttacac caattgaaaa tactaccata tcacagtcaa
     8101 cactatctcc atctttaaat acaacttttt gagcttttcc atcaccttca ataccatcaa
     8161 ctgctacacc caatcttaca tctattcctg tatctcttac acattgttct aatattttac
     8221 taccttcttt atcaagttgt tttgatagta tgctatccat ggcttcaact actactactt
     8281 ctaatccttt tagtctaaat tcccatgcag cttcaagtcc caataaaccg ccaccaatca
     8341 ctacaacttt cttagatttt tctagtgctt cttttacact atataaatct tttatatttc
     8401 taagagtaaa cacattttct aaatcatgac ccttaattgg tggtataaag tttctacttc
     8461 ctgtagctat tacaagctta tcaaatttaa tagctccatc attcactata atagttttat
     8521 tcacttcatc taatttatct acattcgttc ctaatattac tactatatta tttttatcat
     8581 accaatcttt atcttccatg tagaaatctg aaccaagttc ttcatttata ccatcagaaa
     8641 gtgcaggtct atagtaagtc aactcatctt cattagaaat catagttatc ttacaagttt
     8701 tatttctttt tcttatagca tctgctgcat aaaaaccagc tgctccattt cctactatta
     8761 cgaaatattc atcagtatct tttctaaaag taacttcatc ttcttcaact tctataaatt
     8821 ggtcttcctt agctccacat acagggcata cactaggagc actatcacct tcaaaaactt
     8881 cattacaaat tacacattta aatttcttag ttttcttttt acttccaaaa gtagaattta
     8941 acttatctag gaatttacct gtataagaat gtaatttatc aatctcttca tttgatggtt
     9001 taaagttaac agcaaaaggt tttactgctg tcattctaag ctgacttatt ctctccatag
     9061 cattttctat accttctcca ctccatccat atgaaccaaa tactgaagca acttttccac
     9121 catgaagaac tgggtttaac gatattaata tatcccatat aggctttaac gcatctccta
     9181 atattgttgg tgttccaaat aatacacctt gtgatactgc tatttttgcc aaaatatctt
     9241 cttgcttatg ctctattaca tcaaataaat ttactttata gttgctattt ttttcaatat
     9301 atgcttttat agcttctgcc ataatctttg tatatccatg tgcagaaaca taacaaattg
     9361 taacttcttt ttctaacttt atttgttcat ttacactcca gttgtaataa aggtctataa
     9421 tttttctagg attttcagtt aatactggac catgacctgg acatacagta tctatatcta
     9481 aatcttttat cttttctata gcagttacca tagatggttt aaatggaccc attatacaat
     9541 cataatagta tcttaaagca tccaagtaat ccttttcttc attttcatca agtgtattta
     9601 ctatcttatc attactatag tgactaccaa atgaatcaca tgttacaaga gttttatcct
     9661 cttttatata tgtgtatatg gtatctggcc aatgaagcat aggtacagag aaaaattcta
     9721 aagttttgtc cccaattgat aatgtatctc catctgtaac tgcaacatat tcaaagtctt
     9781 tgtttacgat ttccttaaga tatttaattg ctgtttctga agctactact tttgcatttt
     9841 tagctaaatc caaaagcttt tcaacgcttc ctgcatggtc tggttcagta tgacttacaa
     9901 caatatagtc tattttttca aaatctatat ttaaatcatt taatctttca atataattgt
     9961 caaaatgctt atctttaact gtctcaaata aaactgtttt ttctgttcct tttaatacat
    10021 atgaattata agtagttcca taaggtgtat acataattat gtcaaatact cttaaatcag
    10081 ggtcaagcga acctacccaa tataaatttt cttttatttt tagtgatttc aatgacatac
    10141 cgtactcctc ctaaaatagt tatttagtct ctttataccc tgcttgttat attttaaaca
    10201 aaatttattc aaaaaagaaa ttattttaaa gatagtttaa atcttctaat cttctttttc
    10261 taccttcaat acagttccct ttttagaatc tacttttatg tcatattcat ttttatcttt
    10321 atctattctt attttataaa aagtaacatc taaatctttg tctaaactcc attctttaac
    10381 tattccattt tgttctttta atgcaatttc catagcttgt tgaggtgtca ttatgtcatt
    10441 taaatctaat ttttcttttc tagcaactcc atttaaatct tctgaatcca acttttctgt
    10501 tttatcttct aatacatctt ttgtattagc atcaattttc atcttatatt cattattatc
    10561 atctacacct tccacagtat aaacaaattt atttaaatct ctctcaagac ttaaatctac
    10621 tatatcagca tccttatatt tatctttaaa tatgtttatt gcatctgtgt atggcacttt
    10681 aaaatcttgt tccattaaat tttcattatt tttttcatct tttaaagcgt tatttgatgc
    10741 atttgcctcc ttttttgtat tatcttgtgc ttgtcctgaa tccttagttg gactcccact
    10801 acatgcagtt aataaacccg ctataagcat acttaccact aataaatacg tttttttaca
    10861 cataaaaacc ctccttaaat atattaattt attctatatt atgccctcta aaccctttat
    10921 ttaccctttt atattaaaaa ttcctcctat tttatcaatt ttaaaacttt atatttacta
    10981 aaattaaagt tacataaaaa ttatactgtc tcaaaatgga aacaagcctc tttttcattg
    11041 ttgaaataat aaaaatatat tttatttcgt acaaaaagag tgcctcatga actaaaaagt
    11101 tcattttgag acactcccat ttttttatat ttttacttac aaaataacag tacttattaa
    11161 ttttttacaa accttataag tatatctata tgatattctt aaatttaaac actatattaa
    11221 aaaattaaat tttgtttaat tcttttaccg ttaaatccat tttaggatat ggaatcgtaa
    11281 ttccttcttc gtcaaactta actttaacct cttctagtaa atcaagataa attgtccaat
    11341 aatcttctgt cttgcaccaa actctagttg caaatttaat agcattatct ccatgttcta
    11401 ctactcctat aaatggttct ggttcattta ttattaattt atgattatta acaatatctt
    11461 taagtactct tctaacatgt agtatatcat cttcataccc tacactaaaa gtcaaatcaa
    11521 ctcgtctagt attctttgct gaatagttta ttagactatc atttgataca gaaccattag
    11581 gtataagtat ctgtttatta tctggagtaa ccaattgtgt ataaaaaaga cctatttgtt
    11641 caaccgttcc tccatgacct gctgcttcta cgtaatctcc tactttaaaa ggtctaatca
    11701 atagtataat aaatcctcca gcaaaatttg atagactacc ttgtaaagcc aaacctattg
    11761 ctacccctgc tgatgctatc acagcagcaa aactggacaa actcattccc caatattcca
    11821 gaacaatgat tataacggca actttaagta cactcaaaag aagagacttt aaaaactttc
    11881 ttaaagttaa atcaatatct cttttactaa gtaataacat aacatggttt acaaattttt
    11941 ttattatttt aaatccaata gaaagtataa gtatgcctat tataagtttt actccgcttg
    12001 tagtagccca ttctagcaga ctttccatta tacctcccat atccatctta ttaatatcta
    12061 aattatttaa atctaacttg cttatatcat ctattttcgt cgtatccata aaaaactcct
    12121 taccttatat tttagaacca atattatagt ttaaagttct tattttctat ttaatataat
    12181 acaataccat ttgtacatag taaacaagat ttatattcca attatacata ttaggaaaat
    12241 gcaaactatt tgttatatat atgcaaaata taatactaaa ttaaactatt aataaaacac
    12301 tatatagcct taaaatcatc attttttacc cctatatgag atgatactat agttataata
    12361 cttagtacta taaatactac aaaacctttt ttatatccat caactatatt
//
LOCUS       DBGJFF010000116        12125 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.118, whole genome shotgun sequence.
ACCESSION   DBGJFF010000116 DBGJFF010000000
VERSION     DBGJFF010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12125
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.118"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            556..1734
                     /locus_tag="ACOIAF_003019"
     CDS             556..1734
                     /locus_tag="ACOIAF_003019"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HGZ2670020.1"
                     /translation="MKYHIFLIITTLLIIELCNIYICKIASFRNKNITIHKNYKYIFL
                     IKPLTFLIIIFTKLPLPLYYILFYIVSHKLLLSRKSSTNNHFIPNMAVISFCILHLIT
                     VSTISLVKNTSPDIIFANMTYKLISILIASILDFILWYSMNKIKEHIYIITQNQIEFS
                     LFLKFIWFTLGYILFDSIQITFAPPSSLTSYFILGSNILLILQLIIFINHIYSIEKNI
                     YLEKEHNRLKMIKQEQLLKVNLLKKKANIDELTSAYNRKFAFEKIQHYIDNNLLFSLI
                     YIDLDKLKTINDTYGHLAGDNYIINFSSIVIENLGIDDIFARIGGDEFIVIMPKSNEN
                     TTKEFIDKIRKIMDNTISFSYGIVEVPSENKMTVEEIINIADIRMYTNKQNNHKRNNN
                     "
     gene            2066..2965
                     /locus_tag="ACOIAF_003020"
     CDS             2066..2965
                     /locus_tag="ACOIAF_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020397798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HGZ2670021.1"
                     /translation="MSIVLNMPAYLFDSNSIANIYRVFPISFSFIVTGLYFSYKNNKN
                     NKINFEAVLEDKKNLIFLLSLMFGMYLYLCLSLSIYYTVGNVLILKLYGIKSAIIVLV
                     GNYIAERYSFRITQIKGYDIKNQIEYVESVKTEYEINKLNDIVYTDLLTGFYNRPFVN
                     QKLSEWTSNHYKFTLCFTDLNSLKPVNDKFGHQFGDKYISTTAQIIKKLINVENSLLF
                     RYGGDEFLFLLQNTSCVEAEKIMININYELSKLSNTDEYPYALSISYGLVEWDGISDI
                     ETLISKADSLMYENKLKNKSTLN"
     gene            3174..3323
                     /locus_tag="ACOIAF_003021"
     CDS             3174..3323
                     /locus_tag="ACOIAF_003021"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HGZ2670022.1"
                     /translation="MTTVAEGIETSFQKDFLQEINCDMLQGYVFSRPLPIKDFEKLMF
                     QNSSN"
     gene            complement(3583..6150)
                     /locus_tag="ACOIAF_003022"
     CDS             complement(3583..6150)
                     /locus_tag="ACOIAF_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HGZ2670023.1"
                     /translation="MIYYVLPLEHKQATIEIVGGKGMSLSKLLTAGIPVPDGFHVTTA
                     SYQIFVETNHIQSRINKLLDGIDSNNTSQLEDVSKKIGELFHNGEMPQEVSDAIKMAY
                     AGLGNISMAVRSSATAEDLPDASFAGQQETYLNIQGEDKVIDSVKRCWASLWTARAIA
                     YRVKNNIKHEIVALAVVVQKLAFSDSSGIMFTLNPINGRRSEMIINAAWGLGEAVVSS
                     LVTPDTIVVDKDSERIISYEVANKEIMTVRTSEGTEETMVPERLRKKYALTRNQVMQL
                     IQLGKKIEKYYQMPMDVEWALEKDKLYIVQARPITVLPPEWVLPEQDVVYTKGSLAEH
                     LPNPVTPLFATLGLEIVNRASELLWIDMFGKSAKKLLPKNGAYTVINGYVYLSAKSKP
                     LLIAIKSLSPRSLRRTLMNSVARWETARKGYEDAIKQWEEKPLHTMNAHQIMEGIQTI
                     FYAACIYFTRIQLTLPAASISETLFTKLFQGAVRRAGITDTSVFLLGFDTIVLQSEKE
                     LWGISEWVKQNNSINLYLQNSPAIKIAEDFMSSVVPVGVSQEVWIEWKNRINKYFKEF
                     GRTAYEFDFAHSTPQETLTPTFESVKTFVEGKGESPFLRQTTFEKRRKHAEEEILQHI
                     GGLRKKLFFKLLHWAQETSPMRENAIYLMGMGHPLIRCMFQEISERFIRGGAISHLDD
                     IYWLTKSELEVLIEQLDKNKSLSDMNGIIPVRKAELKKYMGYVSPSKLPEKNKKTISQ
                     AQQKQKDGKIVLTGIGTSTGVVTAPACVLNSPADFESFQPGSVLVAVTTTPAWTPLFS
                     SASAIVTDIGGPLSHSSIVAREYGIPAVMATHIATRTIKSGQMITVDGLAGTVTFDE"
     gene            complement(6153..6758)
                     /locus_tag="ACOIAF_003023"
     CDS             complement(6153..6758)
                     /locus_tag="ACOIAF_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HGZ2670024.1"
                     /translation="MPKGIVLTPEQQAERREKIVAVALSLMAENGFQKTSMREIAVLA
                     NMGKSSLYDFFKTKDEIVVYAVEKEIEKTIQQVHRIIDGEPSPQQCLRKIMLNHLRFP
                     EQYRTVLMWLNTESDYLEEEYRKRLKDARYAYQDIIKSVIDNGVTTGIFRKTDTALVT
                     RLLINSMLSISYTSRPSDSSEKMLDETMNIFLHGIMNDGGE"
     gene            complement(7144..8604)
                     /locus_tag="ACOIAF_003024"
     CDS             complement(7144..8604)
                     /locus_tag="ACOIAF_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HGZ2670025.1"
                     /translation="MFNELKTKENVYKNLINGKWVESNSRKPIEIYSPIDNSLVGKVQ
                     SMTKHEVDEVIKNTKESIKVWAEMPVYKRANIFHKAADLLLENIDEIANILVLEIAKD
                     IKSARAEVERTADFLRYTADVGKNMEGEAISGDNFPGGTRNKMSYVSRVPLGTVLAIS
                     PFNYPVNLSMSKIAPALIGGNAVVLKPATQGAISALHVVEIMRKAGIPDGVLNTITGR
                     GSEIGDYVVTHKGINFINFTGSTEVGQHISKISGMVPLLLELGGKDAAIVLEDADLDF
                     AAKNIVSGAYSYSGQRCTAVKRILVQESVADKLVGKIKPLVEKLTIGNPMDEVVITPL
                     IDNKATDFVQGLVDDALHKGAKLITGNVRKNNLFYPTLLDNVNVDMKIAWEEPFGPVL
                     PIIRVKDINQAIEIANQSEYGLQSSVFTSDIDKAFYIADKLEVGTVQINNKTERGPDH
                     FPFLGVKASGMGTQGVKYSIEAMTRPKAVVVNVREL"
     gene            8943..9521
                     /locus_tag="ACOIAF_003025"
     CDS             8943..9521
                     /locus_tag="ACOIAF_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HGZ2670026.1"
                     /translation="MAKSFTEIEKDNIRKRLISECEINWSKYGYKKTNIDELCSKSGI
                     AKGSFYTFFDSKEKLFFEVLNGIQNRLISSVDEILSNMPPKEGFAKALKMLYRLYDKN
                     PFLYSPNNPDYITLLNKIPKEMISQLEYSNVQSFYSLIERHNLKLKIEKEKAFGVCNA
                     LLSSLLLKEDIGYDYIEVFDFMVDNLVEHMLE"
     gene            9572..9805
                     /locus_tag="ACOIAF_003026"
     CDS             9572..9805
                     /locus_tag="ACOIAF_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670027.1"
                     /translation="MIELIQRLNDIIYKINDKLNIHTKLVDPSSEFMPLYSMLSIIVS
                     IFLIILGIKVRKKVLIIIAIISIITNILIILDR"
     gene            9814..11064
                     /locus_tag="ACOIAF_003027"
     CDS             9814..11064
                     /locus_tag="ACOIAF_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGZ2670028.1"
                     /translation="MVKSSNTFNKNFNLMILGQIVSLFGASILKFALSLYILDITGKA
                     EIFATILAVSSIPIIVFSPIGGAIADIFNRRNLMVIFDFSSSLTVLILAVFLFNNNGS
                     ILLVGIIMTILSVISTMYQPTVQSSTPLLVDNEHLMNANGIVAGVASLTNIAGPVLGG
                     VLYGVIGINAIIVISCVSFFLSAIMEIFIQIPFIKQDQNSNIIKTIFTDIKDGIVHIC
                     KENKFILKILFLAIAINLFLSSMLIVGIPYIVKITMGASNFLYGLAEGAISFSSLVAA
                     VSIGIFSKHLKITNLHISFIICAVMFMPMAISVYPFILNTGFPVPFLIFIVSSMLIFF
                     TTTLISIYVITVTQLKTPNELLGKVMSILFAGTAIAMPLGQVMYGKLFDLLSDRVYII
                     ILGVGFITLLIAFVSKITLRNENL"
     gene            11225..11794
                     /locus_tag="ACOIAF_003028"
     CDS             11225..11794
                     /locus_tag="ACOIAF_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HGZ2670029.1"
                     /translation="MSYMPTLEQAWEILRKYNKEEFHIRHAQIVSGVMRYYAKEYDPE
                     RVEFWEIVGLLHDLDFEMYPDEHCVKQRELMTELDLDKSIIDSTISHGYGLTGSDVKP
                     EQFMEKVLFAVDELTGLIGAAAIMRPSKSVSDLELKSVKKKFKDKSFAAGCSRDVIRS
                     GAEMLGWELDKLIQTTIEAMRSLIPEMAI"
BASE COUNT         4198 a         1993 c         1678 g         4256 t
ORIGIN      
        1 aattactata cttagacaca taactactat atttactata tgtaattata ctatacgtgg
       61 acatatagtt atcatacata ctatatgtaa ttactatact tggacacata attattatat
      121 ttgctatgtg taattatact atacttggac atatagttat catacatact atatataatt
      181 actatactta gacacattat catacatact atatgtaatt actatactta tacacatagt
      241 tatcatatat actatatata attactatac ttgaacacat agttatcata catgctatat
      301 gtaattacta tacttataca cataactatt atactttcca tacataatta ctatatttgg
      361 acacatagtt atcatacata ctgtatatta agatataata ctttttaaat tttatataac
      421 acagacacat gcaataaaaa agaaggtatc cataataagc tctttgcatg tacctaaata
      481 aaaactgtaa aatagaatat ataaaatcta atctatttta agtaaataca atttcacttt
      541 aaaagaggat gtactatgaa ataccatata tttttaatca taactacttt attaataatc
      601 gaactatgta acatttacat atgcaaaata gcatctttta gaaacaaaaa tataactata
      661 cataagaatt acaaatatat ttttttaata aaaccattaa ccttcttgat aataatattc
      721 acaaaattac cattaccact atattatata ttgttttaca tagtaagtca taaattgctc
      781 ttaagcagaa aaagttctac taacaaccat tttattccca atatggcagt aataagtttt
      841 tgtattttac accttattac tgtaagtaca atatcacttg ttaaaaatac aagtccagat
      901 ataatatttg caaatatgac ctataagtta attagtattt taatagcttc tattttagat
      961 tttatcttat ggtactctat gaataaaata aaagagcata tttatataat aactcaaaat
     1021 caaatagagt tcagtctatt tttaaaattt atctggttca ctttaggata tatattattt
     1081 gatagtatac aaattacatt tgctccacca agttctctaa cttcctactt tatattggga
     1141 agtaacatat tattaatctt acaactaatt atattcatca atcatatata ttccattgaa
     1201 aaaaatattt atttagaaaa agaacataat agactaaaaa tgataaaaca agaacaatta
     1261 ctaaaagtaa atttattaaa gaaaaaagct aacattgatg aattaacatc agcatataat
     1321 cgaaaatttg cttttgaaaa aatacagcat tacattgata acaatttatt attttcattg
     1381 atatatatcg atttggataa attaaagacg ataaatgata catatggaca tttagctggg
     1441 gataactata ttattaactt ctcaagtata gttattgaaa acttaggaat agatgacata
     1501 tttgctagaa taggaggaga tgagtttata gttattatgc ctaaatctaa tgaaaacacc
     1561 acaaaagaat ttatagataa aatacgtaaa attatggata atacgatatc ttttagctat
     1621 ggtattgtag aagttcctag cgaaaacaaa atgactgtag aagaaattat taatattgct
     1681 gatattagaa tgtacactaa taaacaaaat aatcataaaa ggaacaataa ctaaaatgaa
     1741 tatttacatg agcttacttg taatcttact atgcctttta tcttattcta tctatcatta
     1801 taattttttt aaatatctag acataatgat taataactct aaaaataaat acaaagtata
     1861 tattgtatac tttcttttta attatttaat ttttattatt tgtacttatt taaggttaca
     1921 tctagttata aattggacaa tttttatgtt aattttattt atgcaaatta aagtaactta
     1981 caaactttcc acaataaaaa acctttttta ttcgttaaac tgctccatca ttggtttagc
     2041 aataaatata ttttttaggt ctttaatgtc aatagtgtta aatatgccag catatttatt
     2101 tgatagtaat tctattgcaa atatttacag agtatttcct atatcattta gctttatagt
     2161 tacaggacta tatttcagtt ataaaaataa taaaaataat aagattaact ttgaggcagt
     2221 tttagaggac aaaaaaaact taattttctt attgagcctt atgtttggaa tgtacttgta
     2281 tctatgtttg agtttgtcaa tatactatac tgttggaaat gtattgattt taaaattata
     2341 tggaatcaag tctgccataa tagttttggt aggtaactat attgcagaaa gatatagctt
     2401 tagaattact caaataaaag gttatgatat taaaaatcaa atagaatatg tagaaagtgt
     2461 aaagacagaa tatgagatta ataaattgaa tgatattgta tatacagatt tactgacagg
     2521 cttctacaat agaccttttg taaatcaaaa gctatcagag tggacatcaa atcactacaa
     2581 atttacctta tgttttactg atttaaatag cttaaaacct gtaaatgata aatttggaca
     2641 ccaatttggt gataaataca tatccactac tgctcaaata ataaaaaaac ttataaatgt
     2701 agaaaattcc ttactattta gatatggtgg agatgaattt ttattccttt tacagaatac
     2761 aagttgtgta gaagcagaaa agataatgat taatataaat tatgaattat caaaattaag
     2821 taatactgac gagtatcctt atgctctttc cataagctat ggattagttg agtgggatgg
     2881 aatatccgat attgaaactt taatatcaaa ggcagattct ttgatgtatg aaaataagct
     2941 taaaaataaa tctactttaa actagctagt taagatattc tacaaaatat atgttataag
     3001 agagtactaa gaatgtcaaa atatatagat tttgcaaata ttgaatacag tttactaatg
     3061 aatgcactgg atataagtgt tagtaaacac ctattagatg aacactttac tgttgtttgg
     3121 gctaatgacc gttattatga aatgttcggc tatacctagc taaaaagtta aaaatgacca
     3181 ctgtagccga gggaatagag accagttttc aaaaagattt cttgcaagaa ataaactgtg
     3241 atatgttaca aggatacgtg ttctctagac ctttaccaat aaaagacttt gaaaaactta
     3301 tgtttcaaaa ctcctccaat taacaatata aaattgctta ttataagtga tattattgta
     3361 tcaacaaata aaggaattgt ctcaaaattg attttttcta atcaaatttt tattttgaga
     3421 cattctcatt tttttgcata agaaaatacc caccaagcta cggtttttct taccgctgtt
     3481 cggtgggtat tagtagtatt atgaaatctg aaaactttaa gtattactac ttccgctagc
     3541 gcaatcacaa aggtcgaaag cttcaaagcg aaaataggct ttttactcat caaacgtcac
     3601 cgtacctgcc aacccgtcca ccgttatcat ttgtccgctc ttaatagttc gtgtggcaat
     3661 gtgtgtagcc ataacggctg ggatgccata ttcacgtgcg acaatgcttg aatggctaag
     3721 aggaccacca atgtccgtta caatcgcact tgcagatgaa aataatggtg tccatgctgg
     3781 agttgtggta actgcaacca aaacacttcc aggttgaaag ctttcaaaat ctgctggact
     3841 attcagtaca caagctggag ccgtcacaac accagtactg gtaccaatgc ccgtcaacac
     3901 aattttccca tctttctgct tctgttgtgc ttgtgatata gtttttttgt ttttctctgg
     3961 caacttggat ggtgacacat aacccatgta ttttttaagt tcagcttttc gaacaggtat
     4021 tatcccgttc atatcagata gagacttgtt tttatccaac tgctctataa gtacctccaa
     4081 ttccgatttt gtgagccaat aaatatcatc taaatgtgaa atagctcctc ctcgtatgaa
     4141 tcgttctgaa atttcctgaa acatacaacg aatcagagga tgtcccattc ccatcaaata
     4201 aatagcattt tcacgcatag gagaggtttc ctgtgcccaa tggagcaact taaaaaatag
     4261 ttttttacgc aatccaccta tgtgttgtaa aatctcttct tctgcatgtt ttctacgctt
     4321 ttcaaacgtg gtctggcgta aaaaaggact ttcccctttt ccttccacaa atgtttttac
     4381 ggattcaaaa gttggtgtaa gtgtttcctg cggtgtggaa tgcgcaaaat caaactcata
     4441 agcggtacga ccaaactctt taaagtactt attgattcga tttttccatt ctatccatac
     4501 ttcctgtgaa actccaacag gcacaactga agacatgaaa tcttctgcta tctttattgc
     4561 aggactgttt tgcagataaa ggttgatgct gttattttgc tttacccatt ctgaaatacc
     4621 ccacaactcc ttttccgatt gcagaacaat cgtatcaaac ccaagcagga aaacggaagt
     4681 atccgttata ccagcacgtc gaactgctcc ttgaaataat tttgtaaata atgtttcact
     4741 gatagaagca gcaggcaatg taagctgaat ccttgtgaaa taaatacatg cggcataaaa
     4801 tattgtctga ataccctcca taatttgatg ggcgttcata gtatgcaggg gcttttcttc
     4861 ccattgttta atagcatcct catatccttt tcgtgccgtt tcccagcgtg caacgctgtt
     4921 cataagtgtc ctacgtaaag agcgtgggga aagggatttg atagcaatca aaagtggctt
     4981 tgatttagca gaaagataaa catatccatt aataaccgta tatgccccgt tttttggtaa
     5041 taattttttc gcacttttac caaacatatc tatccataaa agctctgatg cacggtttac
     5101 gatttcaaga ccaagcgtag cgaacaaagg tgtaacagga tttggcaagt gttctgccaa
     5161 acttcccttt gtgtatacaa catcctgttc tggcaatacc cattctggtg gcagaacagt
     5221 aattggacga gcctgaacaa tgtacaattt atctttttcc agtgcccact ctacatccat
     5281 aggcatttga taatacttct caattttctt tccaagctgt atcagttgca ttacctgatt
     5341 acgagtaaga gcatattttt ttctcaacct ttcaggaacc atggtttctt ctgttccttc
     5401 tgaagtacga accgtcataa tctctttatt tgccacttca tacgatataa ttcgttcgga
     5461 atccttatcc actacgattg tatcgggtgt aactaagcta gaaaccaccg cttcaccaag
     5521 cccccatgca gcgttaataa tcatttcgct acgtctacca ttgattgggt ttaacgtaaa
     5581 cataatacca gatgaatcgg aaaatgcaag cttttgtact acaacagcaa gtgcgacaat
     5641 ctcgtgcttt atattattct tcacacgata agcaatagca cgagctgtcc ataaggaagc
     5701 ccagcatctc tttacagagt caataacttt atcttcacct tgtatgttaa gataagtttc
     5761 ctgctgacct gcaaaagaag cgtcaggtaa atcttcagca gtcgcagacg aacggacagc
     5821 catcgatatg ttccctaatc cagcataagc cattttaatt gcgtccgaca cttcctgtgg
     5881 catttctcca ttatggaaaa gctcccctat tttcttagat acatcttcaa gttggctggt
     5941 attattggaa tcaataccat ctagcagttt attaattcga gactgaatat ggttcgtctc
     6001 aacaaaaatc tggtatgaag cagtcgtaac atgaaaaccg tctggcacag ggatgcctgc
     6061 tgtcaatagt tttgataaag acatcccttt tccaccaaca atttcaatgg ttgcttgttt
     6121 atgttccagt ggcaagacat aataaatcat aatcattcac ctccatcatt cataattccg
     6181 tgcagaaata tattcattgt ttcatccaac attttttccg aactatccga tggtcttgac
     6241 gtgtaactga ttgataacat ggagttaatt aacaaacgtg tcacaagagc ggtatccgtt
     6301 ttgcgaaaga tgcctgtggt cacaccatta tcaattactg attttataat atcttgatat
     6361 gcataacgag catctttaag tctctttcga tactcttctt ctaaataatc agattctgta
     6421 tttaaccaca tcagtacagt tctatactgc tctggaaatc taagatgatt aagcatgatt
     6481 tttctaaggc actgttgtgg tgaaggttca ccatcaataa tcctatgaac ttgttggata
     6541 gttttttcta tttctttctc aactgcatat acaacaatct cgtctttcgt tttaaaaaaa
     6601 tcgtatagac ttgactttcc catgttggct aaaactgcaa tttctctcat tgatgttttt
     6661 tggaatccat tttcagccat gagactcaaa gcaacagcaa ctattttttc tcggcgttct
     6721 gcttgttgtt cgggtgtaag cacaattcct tttggcatag ggcaatacct ccttttagcg
     6781 accactggtc gtttcttttt tattcatcat acagcgacca acggtcactt gtcaaggtta
     6841 atttttttca ttgtatatgg tactcgagat aaaaatgttc attttagaaa ttgctctatt
     6901 taagtagaaa taggcttcat cattagaaaa caagactata aatttgagat atttatcatg
     6961 ttcaaatttc atcaatatca aagtttaaga tattaataaa aaaagaatta ctaagaaata
     7021 acatataaag attcaaaaaa ggagttgaac ttaaaattca attacaaaaa atcaaattta
     7081 agacaactcc tttattcttt atctctacta ttctttatct ctactattct ttatctctat
     7141 ttattacaac tccctaacat ttaccactac tgccttaggt ctagtcatag cctcgattga
     7201 atacttaaca ccttgagttc ccataccaga tgcctttaca cctaaaaatg ggaagtggtc
     7261 tggacctctt tcagtcttat tatttatttg aacagtacca acttcaagtt tatcagctat
     7321 ataaaatgcc ttatcaatat cacttgtaaa tactgatgat tgaagcccat actctgattg
     7381 attagcaatt tcaattgctt gatttatatc tttaactctt ataattggaa gtactggacc
     7441 aaatggttct tcccaagcaa ttttcatatc aacatttaca ttatctaaca atgttggata
     7501 gaataaatta ttttttctaa cattgcctgt tattaacttt gctcctttat gtaatgcatc
     7561 atcaaccaac ccttgaacaa aatcagttgc tttattatct ataagaggag tgataactac
     7621 ttcatccatt ggatttccaa ttgtcaactt ctctaccaat ggctttattt ttccgaccaa
     7681 tttatccgcc acactctctt gtactaaaat tctctttact gccgtacatc tttgtcctga
     7741 atatgaatac gctccagata ctatattctt agctgcaaaa tctaagtctg catcttctaa
     7801 tactatagcc gcatcttttc caccaagctc caataataat ggtaccattc cagatatttt
     7861 agatatatgc tgaccaactt cagtactacc agtaaagtta ataaaattaa ttcctttatg
     7921 cgttacaaca taatctccta tctcactacc cctacctgta atagtattaa gtactccatc
     7981 tggtatccca gcttttctca taatttctac cacatgaagt gcgctaatag ccccttgtgt
     8041 agcaggtttt aatacgactg catttccacc aataagtgct ggagcaatct ttgacattga
     8101 taaatttaca ggataattaa aaggagatat agctaaaaca gtacctaatg gaacccttga
     8161 aacataagac atcttatttc ttgttccacc agggaaatta tctccactaa tggcttcacc
     8221 ctccatattt tttcccacat cagctgtgta tcttaaaaaa tctgctgttc tctcaacttc
     8281 tgcccttgca gattttatgt ctttggctat ttcaagtacc agtatattag ctatctcatc
     8341 tatattttct aacaataaat cagcagcctt atgaaatata ttggctctct tatatacagg
     8401 catttcagcc catactttta tactctcttt tgtgtttttt ataacctcat ctacctcatg
     8461 ttttgtcata gattgtactt tcccaacaag agaattatct attggtgagt aaatttctat
     8521 tggctttctc gaattagatt ctacccattt tccatttatt aaatttttat atacattttc
     8581 ttttgtttta agttcattaa acatattttt attcctctct ctgatataac tttgtgtatt
     8641 taaattatag ccatttattc gttatgtaaa catttctttt tttcataaac gtttacctat
     8701 aaaaaaattt taaagttaac gtttttctac attcttctat tttaataaca tactcgctcc
     8761 aaatctatat aggtcacact ttaatatcaa tataccaata taccaatata ccaatatacc
     8821 aatataaata ttataaaaat aaatcacttg acaaatttat aagtagacaa tattatatat
     8881 ctatgactta aaatattttt atagtcatag atatcatata tagacagaaa ggattgataa
     8941 aaatggctaa aagttttaca gaaatagaaa aagacaatat aagaaaaaga ctaatttcag
     9001 aatgtgaaat caactggtct aaatatggat acaaaaaaac taatattgat gaactttgtt
     9061 caaaatcagg aattgcaaag ggttctttct atacattctt tgattcaaaa gaaaagttat
     9121 tttttgaagt actaaatgga atccaaaaca gacttatatc atcagtagat gaaattttaa
     9181 gtaacatgcc tccaaaagaa ggctttgcaa aagccttaaa gatgttatat cgattatatg
     9241 ataaaaatcc atttttatat tcaccaaata atcctgatta catcacttta ttgaataaga
     9301 tacctaaaga gatgatttca caacttgaat acagcaatgt acaaagtttt tatagcttaa
     9361 ttgaaagaca taatctcaag ttaaaaatag agaaagaaaa agcctttggt gtttgcaatg
     9421 ccctactgtc aagcctattg ctaaaagaag atattggata tgactacatt gaagtatttg
     9481 attttatggt agacaatctt gtagaacaca tgcttgagta ataatttaaa ataaattttc
     9541 taaacatttc tttataacta ggaggggtaa tatgattgaa ttaatacaaa gactaaatga
     9601 tattatttac aaaataaatg ataaactaaa tatccacaca aaacttgtag accctagcag
     9661 tgagtttatg ccactttact ctatgttatc tataatcgtt tctatatttc taataatatt
     9721 aggtattaaa gtaagaaaaa aagtccttat aataattgct attatatcaa ttattacaaa
     9781 tattttaata atcttagata ggtaggtaca tatatggtaa aatcctcgaa cacttttaat
     9841 aaaaacttca acttgatgat actaggtcag atagtttcat tatttggtgc atcaatactt
     9901 aaatttgctt tatctttata catacttgat ataactggta aagcagaaat atttgctact
     9961 atactagcag tatcttcaat cccaattata gtattctccc ctattggtgg agctattgcg
    10021 gatatattca acagaagaaa cttaatggtt atttttgatt tttcaagtag tcttacagta
    10081 ctaatcttag cagtattctt attcaacaat aatggttcaa tattacttgt aggaattatt
    10141 atgaccatat tatcagtcat aagcacaatg tatcaaccaa ctgttcagtc aagtacaccc
    10201 cttttagttg ataatgagca tttaatgaat gcaaatggta ttgtagctgg agttgcatct
    10261 cttacaaata ttgcaggacc tgtattagga ggtgtcttgt atggtgtgat tggaattaat
    10321 gcaattatag ttataagttg tgtaagtttc tttctatctg caattatgga gatatttata
    10381 caaattcctt ttatcaaaca agatcaaaac agcaacatta taaaaactat atttactgat
    10441 ataaaggatg gtattgttca tatatgtaaa gaaaataaat ttatactaaa aatacttttt
    10501 ttagctatag caataaactt atttttaagc tctatgttaa tagttggtat cccctatata
    10561 gtaaaaatca ctatgggagc tagtaacttc ttatatggtc tagcagaagg agcaatttct
    10621 tttagttcac tggtagctgc tgttagtatt ggtattttct caaaacatct aaaaatcact
    10681 aatttgcata tttcctttat tatatgtgca gttatgttca tgccaatggc gatttcagta
    10741 tatccattta tattaaacac aggatttcca gttccatttt taatctttat agtatcatct
    10801 atgctaatat tttttacaac gactcttatc tcaatatatg ttataacagt cacacaatta
    10861 aaaactccaa atgaactctt aggaaaagta atgtcaattc tttttgcagg aacagcaatt
    10921 gcaatgcccc ttggtcaggt tatgtatggt aaactttttg atttattatc agatagagtg
    10981 tacatcatta tactaggagt tggatttatt acactgctta tagcatttgt atcaaagata
    11041 acattgagga acgaaaatct ttagatacaa agaatttaga tataaagatt taattttcac
    11101 taatttctga atttaaatgc aaaattgtgt atcagtatgt tgacgtttta gaaattgatt
    11161 gatataatca atggaaaata aactttataa aaattcaaaa tattcagcta gaggagtgat
    11221 aaaaatgagt tacatgccaa ctttagaaca agcatgggaa atattaagaa aatacaataa
    11281 ggaagaattt catataagac atgctcaaat tgtatctgga gttatgagat actatgcaaa
    11341 ggagtacgac cctgaaagag tagaattttg ggaaatagtt ggacttttac atgatttaga
    11401 ttttgaaatg tatcctgatg aacattgtgt taaacaaaga gaattaatga cagagcttga
    11461 tttagataaa tcaataatcg attctacaat aagtcatgga tatggattaa ctggttcaga
    11521 tgttaaacca gagcaattta tggagaaagt attatttgct gtggatgaac ttactggttt
    11581 aattggtgct gctgcaataa tgcgtccttc aaagagcgtt tctgatttag aattaaaatc
    11641 tgttaagaag aaatttaaag ataaatcatt tgctgctggc tgttctcgag acgttataag
    11701 aagtggtgca gaaatgcttg gatgggaact tgataagctt atccaaacaa ctatagaagc
    11761 tatgagaagc ttgattccag agatggcaat atagctcctc catacatatg ccatagaagt
    11821 tacatcttta aagagcattt ctctttttga tgcaacttct ttttatattt tgtagaataa
    11881 aaagtttaat attttaaact cactatataa taaataatat aatgtataat agtcataaac
    11941 tttctaacct taaaagtttc taataatcaa taaacaattc taatatacaa atgtcattat
    12001 atgatttaat tatatatctt caatttatat attaaaataa atatatatat attaaattct
    12061 tatacaccaa atttttacat taaacttcta tgcaccacat ttatacatat taaattctta
    12121 tatat
//
LOCUS       DBGJFF010000117        11997 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.119, whole genome shotgun sequence.
ACCESSION   DBGJFF010000117 DBGJFF010000000
VERSION     DBGJFF010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11997
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.119"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(203..1933)
                     /gene="lpdA"
                     /locus_tag="ACOIAF_003029"
     CDS             complement(203..1933)
                     /gene="lpdA"
                     /locus_tag="ACOIAF_003029"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HGZ2670030.1"
                     /translation="MSVEVIMPKAGVAMEEGTIVSWLKQEGEEVKIGEPILEITTDKV
                     NMEIESEGEGTLAVIIHKEEGEVLPVFTVIGVIAEKGENQEEVKAKYLSGNVSKEDTV
                     KENQNIEVKEEKINKKECNHDYDVVVIGGGPGGYLSALKAALLGGRVALVEENILGGT
                     CLNRGCIPTKTYIKTAEILEEIDQLSKRGVKVTVDKEQDIKKAIKYKNRVVKKLTAGV
                     GGLLKSRDVDVFNLKASVKEEHKVILSDGKVLDTENIIIATGSKVRILPIKGIESNLI
                     ITSTEALDLETVPEKLVIIGGGVIGCEFAEIFNSRGSKVTIVEMEDRVIPRMDKELSE
                     SLKYSLSKKGINVLTKKKVSEFKEEGNNILVCIEGEEPIKADLCLYAIGREANLSGIE
                     DLDIKIDKGSIVVNSKMETSIPSIYAVGDVTGGVMLAHAAFKMGEVAASNALGVNKEV
                     DLGALPSCVYTIPEVASVGITEEDARKKYNVKVGKFNFAGNGRALASGQEQGYVKVVA
                     DAKYGEILGIHMFGCGVAELINHAASFKALEIPTDEASELIFGHPCTSEALMEALADV
                     NGECLHLPKK"
     gene            complement(2100..3146)
                     /locus_tag="ACOIAF_003030"
     CDS             complement(2100..3146)
                     /locus_tag="ACOIAF_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HGZ2670031.1"
                     /translation="MVANKIKATPAARSQARKDNIKLDRLIGSGENGRIHLVDVLNYL
                     KDNKANTTPLARRIAEDLNIDLETIVGTGYNGKIRKCDVEKLTGKETIVSTNTSKSSE
                     KKELKIENENSRMFNTVEGIFEKPNPMRATVAKRMSESYFSAPVFTFNIEVDATELKV
                     LRAKLIDTVKESTGVKLTMTDLIVMAVSKILPNHQALNSAWTDEGIFRYKDVNIAIAV
                     GLDEGLYVPVVKNANKKSLKEIAKESKELAEKVKTGKLMPADQEGNTFTISNVGMYGI
                     TTFTPIINMPSSAILGVGATQDKFVPVNGEAKIKPIMNLSLTSDHRVIDGTVAAKFLK
                     DLKELLENPLSMLV"
     gene            complement(3159..4145)
                     /locus_tag="ACOIAF_003031"
     CDS             complement(3159..4145)
                     /locus_tag="ACOIAF_003031"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HGZ2670032.1"
                     /translation="MSTRELTYAQAIKEAMSEEMRRDENVIFMGEDIGIYGGAFGVSV
                     GMIDEFGPERVRDTPISEAAIAGAAAGAAATGLRPIMEVMFMDFVTISMDAIVNQAAK
                     MRYMFGGKAQVPMVVRCPGGSGTGSAEQHSQSLEAWFCHVPGVKVVAPSTPADAKGLL
                     KAAIRDNNPVIFVENKLLYRKKGFVPEDDYVIEIGKADIKREGTDVTVITYGRMLQSV
                     EEAAETLSKENINVEIIDLRTLYPLDKETIVKSVCKTGRVLICHEAAKTGGLGGEISA
                     LITESESFDYLDAPVKRICGKDVPIPYNPELEKAVVPRVDEIEEAIKSLIVR"
     gene            complement(4170..5132)
                     /locus_tag="ACOIAF_003032"
     CDS             complement(4170..5132)
                     /locus_tag="ACOIAF_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HGZ2670033.1"
                     /translation="MSISKETLLEMYKRMNQARKFEEKVSWFFARGMVHGTTHLSVGQ
                     EASSVAAVMALEKGDLVSLTHRGHSQFIGMGIDLNKMMAELMGKETGFCKGKGGSMHI
                     ADIESGNLGANGVVGGGLTIAPGAALTQQYKKTGKIVLCSFGDGASNEGTFHEGINLS
                     SIWKLPIIFYCENNLYGMSTSIKRHMNIESIATRAASYGIEGISIDGYNPIEVYETVQ
                     KAAEKCRRGEGPVLIESRTYRWLGHSKSDANVYRTKEEIESWKAKDPIEFLKNYLIEN
                     NLSNEDELDKIQEFAKQSIEDAVEFAQNSPNPKIESLLEDVYAD"
     gene            complement(5170..6123)
                     /locus_tag="ACOIAF_003033"
     CDS             complement(5170..6123)
                     /locus_tag="ACOIAF_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HGZ2670034.1"
                     /translation="MKKIGDLRLMMKCCSLYYEDNLNQQEIANQLGISRPTISRILKE
                     AFEQGIVKIQIVDVLKNDYQKIERSLERKYKLKEVIVVDDKQDALTQKQELARAVSEY
                     LTRVVKENDIIGVSIGTTLKEIPRYVEKSNCKNVTFIPLLGGIGDNEIDIHANQIAVS
                     LARAFGGDFKLLHAPAVMSDLSTKEKLCKDEKIKEVLDLIDKTTIAIVGIGNPMSLNS
                     TIMASGYMNEADIEDLKKYNSIGAICLQAFDKEGKTSILEFNQRVLGVKLEDLKKIKR
                     TIGVASGDEKIEAIKASLKAKFINSLAINHSLALKLLEDCD"
     gene            complement(6568..7941)
                     /locus_tag="ACOIAF_003034"
     CDS             complement(6568..7941)
                     /locus_tag="ACOIAF_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGZ2670035.1"
                     /translation="MNTEVNVKATTIRYEKISFFERISYGCGDLGCNIIYSAMSAFLL
                     FYYTNYADVSAAAVGSIMLVSRILDGFSDLTMGIIVDRTKSKYGKARPWILRMAIPFA
                     IAAVLLFSVPSNLGITSKLIYIFITYNLVSTVIYTAINVPYATLNSLITQNQYERGVL
                     SIFRMILATCGTLLINGLTLPLVEYFGNNLSAWTKTFFVFGIASIMVFFITFTGTKER
                     VKAVKQDRNEVIPFKIGIKSLFRNKYWIQITLCLVCIFIVFAINGGSSVYYAKFILGD
                     EKLFGPINMVSNISQIIAMFMVAPFIKKFGKRNVLIVGSIILISSNVMFIIVGQNHIG
                     VIFASAIKGIGSAGIASTMFAIVSDTIEYGEWKTGYRTEGLINSASSFGFKVGNGLGS
                     AILGAVLSIGGYVGTSATQSDLAIVSIKVCFIYLPIFITILQIIIMSFYKLDKEYSTI
                     LDELNTK"
     gene            complement(8038..9684)
                     /locus_tag="ACOIAF_003035"
     CDS             complement(8038..9684)
                     /locus_tag="ACOIAF_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HGZ2670036.1"
                     /translation="MIKNPILPGFNPDPCICRKGDDYYLVVSSFEWFPGIPVYHSKDL
                     KNWELYTHILTDETKIDLKKLPSSKGIWAPCLTYCEEEDLFYIVYGIMNSMNARYFDV
                     DNYLITSKDIKGEWSEPVYLHSSGFDASIFHDDDGKKWIASLDWETREGYEKPGVICL
                     VEYCTKKKEIVGYPKRIWSGGTDRGCIEAPHITKRGDYYYIMCAEGGTGYGHSVTMGR
                     AKNIWGPYEKDSMNPIVTSIPGDFYERHDPDHLKPKYYNPESKLQKSGHGSYIETTSG
                     EVYLVHLTSRPFVPELRCTLGRETAIQKMKWTKDNWLRMEDESNLAKEYVSESKLEEH
                     LVSSIPSFDDFDSNELGLQYYAPRISPLSFADVKSRPGYVRIRGQESRTSLNKVSILA
                     RKLTSVYARITTKMEFYPEVHQHSAGLIMYYDNMNYINLRKYYSETLGQSALSIIHLE
                     NGEKTEFLNTRIPIKDIPIYLRLYVQGRKSYFEWSYDEKNYQRIGKVFDTTKFSDEYC
                     KYGEFTGTFIGLTCADRVKHKHYADFDFFEYIVDESKDVD"
     gene            complement(9706..10872)
                     /locus_tag="ACOIAF_003036"
     CDS             complement(9706..10872)
                     /locus_tag="ACOIAF_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HGZ2670037.1"
                     /translation="MYIKGVNLGGWLVLEKWMTSSLFEGTEAEDEYYLPRQLSREAYE
                     SRIKTHRSEYITERDFATIKSMGFNSVRIPVPYFIFGDCEPFIGCVKELDKAFAWADK
                     YGLSILIDLHTVPGSQNGFDNGGISGICSWSQNPEYVAFTLNVLERLAKRYGMRHELY
                     GIQILNEPITERMWNIMNVPNRFKAVDKEMARGSKPNSLEFLRDFYIKAYRVMRPYMR
                     EENVIVFHDAFELKAWKDFMREEEFKNVVLDTHQYLMLAEADGCEQSIDSYLKYIREN
                     YAKDILQMQKYFPVICGEWSLFNSYVCGIDTNGGQSPLNGIESNIDKLSKDDKRELYR
                     KIAKAQLDAWRNGSGHYYWNYKLLLDTVNEEGWIGWDSWDLGKCVAQEWYPIEY"
     gene            11089..11934
                     /locus_tag="ACOIAF_003037"
     CDS             11089..11934
                     /locus_tag="ACOIAF_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGZ2670038.1"
                     /translation="MNRNNFSHEIIEPIDNLDVNFKLFDDSGSYVANHWHNSLEIIYI
                     TSGDLQINMEGYTYNLKANECMFINSGIIHSTRCIYQNTSILLQVPLSFLNKYIPDFK
                     NCYFDFKVNANDNNYKKNSSKVKLILENMREIKLSSPPAANLLFTSLLFELLFELYTN
                     FKISVGNKNMKKTVLDLSKFEPVLEYTKINYKMPISINKIAKVAHLQPEYFCRKFKQY
                     MGQTYLEYLNDVRISHIYKDLINTNDTLCSILEAHGFTNTKLFYRIFKEKFKCTPKHI
                     RKKST"
BASE COUNT         3673 a         2314 c         1284 g         4726 t
ORIGIN      
        1 ataatgttat tttatattac tatctttttt gtacaacaaa aactacaagg attctatatt
       61 ctatattttt ccataaaaaa taccccttat tatgcaaata aattatttgc ataataaggg
      121 gtatggctaa gtaagtgttt taaaatttta tgatataaat taatattaat aatagactta
      181 aattaataat ttatgtttag atttattttt taggtaagtg taaacattct ccatttacat
      241 ctgccaaagc ttccattaaa gcttctgaag tacatggatg accaaatatc aactcacttg
      301 cttcatcagt aggtatctct aacgctttaa atgatgctgc atgatttata agttctgcta
      361 caccacatcc aaacatatgt atacctaaaa tttctccata tttagcatct gctactactt
      421 ttacatatcc ttgttcttga ccagaagcta aagctcttcc atttccagca aaattaaact
      481 taccaacttt tacattatat ttttttctag catcctcttc tgtaatacca actgatgcta
      541 cttctggaat tgtataaaca caactaggta atgcacctaa gtcaacttct ttattcacac
      601 ctagagcatt tgaagctgct acctctccca ttttaaaagc agcatgagct aacattacac
      661 ctccagtaac atcacctact gcatatatgc ttggtatact tgtttccatt ttactattta
      721 caactattga acctttatct attttaatat ctaagtcctc tatgccagaa agattagctt
      781 ctcttcctat agcatataga cataaatctg cttttattgg ctcttcacct tcaatgcaca
      841 ctaatatgtt atttccttct tctttaaatt cagaaacttt ctttttagtt aatacattta
      901 tacctttttt actaagagaa tattttaaag attcgcttaa ctctttatcc attcttggga
      961 ttactctatc ttccatttct actatagtta ctttagaacc tcttgaattg aatatctctg
     1021 caaattcaca accaatcact ccaccaccaa ttattactaa tttttctggc actgtttcca
     1081 aatctaatgc ttctgtacta gttattatta aatttgattc tattcctttt ataggtaata
     1141 tccttacttt ggaacctgta gcaattatta tattttctgt atctaaaact tttccatctg
     1201 ataaaattac tttatgctct tctttaacac tagctttaag attaaacaca tctacatctc
     1261 tactttttaa taatccgcca acaccagctg taagtttttt aactactcta tttttatatt
     1321 ttatggcctt cttaatgtcc tgttctttat ctacagttac ttttacacct ctctttgata
     1381 attggtcaat ttcttctaat atttctgctg tttttatata agtctttgta gggatacaac
     1441 ctctatttaa gcaagtacct cctagtatat tttcttcaac taaagcaact cttccaccta
     1501 aaagagctgc ttttaatgct gataaatatc ccccaggtcc tcctcctatt accactacat
     1561 cataatcatg attacactct tttttgttta ttttttcttc tttaacttct atattttgat
     1621 tctctttaac tgtatcttct tttgaaacat ttccagataa atatttagct tttacttcct
     1681 cttgattttc ccctttttct gctattacac ctattactgt aaatactggt aatacttcac
     1741 cttcctcttt atgtatgatt acagcaagtg taccttctcc ttctgactct atctccatat
     1801 taactttatc tgttgttatt tctagtatag gttctccaat ctttacttct tcgccttctt
     1861 gctttagcca agatactata gtaccttctt ccatggctac tcctgctttt ggcattatta
     1921 cttctactga cataggctct cctcctattt attttcttat attttaatta agcaaatcat
     1981 attcacatat agttactgtt aaaaataatt cttaatatat aaaagcttta aatttactga
     2041 aacaattata ataatataat ctattcaaac tatttatatc agtaacaaaa tatcaaaacc
     2101 tatacaagca ttgataaagg attttctaac aattctttta aatcttttaa gaattttgca
     2161 gctactgttc catctattac tctatggtct gatgttaatg ataagttcat tataggtttt
     2221 attttagcct ctccatttac tggaacaaac ttatcttgtg tagcacctac ccctaagatt
     2281 gcacttgaag gcatatttat tataggtgta aatgtagtta taccatacat tccaacatta
     2341 cttattgtaa atgtattacc ttcttggtct gctggcataa gtttccctgt ctttactttt
     2401 tccgcaagtt ctttgctttc ttttgctatt tcttttaaag actttttgtt tgcatttttt
     2461 actaccggaa catataagcc ttcatctaaa ccaactgcaa tagctatatt tacatcttta
     2521 tatctaaata taccctcatc tgtccatgct gaatttaagg cttgatgatt tggaagtatt
     2581 ttagatactg ccattactat taaatctgtc atagttaatt ttactccagt tgattctttt
     2641 actgtgtcga ttaactttgc tctaagtacc ttcaattctg tagcatctac ttctatattg
     2701 aatgtaaata caggagcact aaaataactt tctgacattc gttttgctac agtagctctc
     2761 attggatttg gcttttcaaa tattccttct actgtgttaa acattcttga attttcattt
     2821 tcaattttca gctctttttt ctcactagat ttagatgtat tagttgatac aatagtttct
     2881 tttccagtta atttttccac atcacacttt cttatctttc cattatatcc agtacctaca
     2941 atagtttcta aatctatatt taaatcttct gctattcttc tagctaaagg agtagtattt
     3001 gctttattat cttttaaata atttaacaca tctactaaat gaatccttcc attttctcca
     3061 cttccaatca gcctatctaa ttttatattg tcttttcttg cctgacttct agctgctggt
     3121 gtggctttta ttttatttgc caccatatct atccctcctt atctaacaat taaagatttt
     3181 attgcttctt ctatttcatc aactcttggg acaactgctt tttctaactc tggattatat
     3241 ggtattggca catcttttcc acaaattctt tttactggag catctaaata atcaaatgat
     3301 tcactttctg ttatcagggc tgatatttct ccacctagcc caccagtctt agctgcttca
     3361 tggcaaatta aaactctccc agttttacat acactcttta ctatagtctc tttatctaat
     3421 ggatataaag ttcttaaatc tataatttca acatttatat tttcttttga tagagtctct
     3481 gctgcttctt caacactttg taacattctt ccataagtaa ttactgtaac atcagtaccc
     3541 tctcttttaa tatctgcctt tccaatttca attacataat catcttctgg cacaaaacct
     3601 ttttttctgt ataacaattt attctcaaca aatatgactg gattattatc tcttatagct
     3661 gctttcaaca atcccttagc atctgctggt gtagaaggtg caactacttt tactcctggt
     3721 acatgacaaa accaagcttc taaactttgt gaatgttgct ctgctgaacc agttccagaa
     3781 cctcctggac atctaacaac cattggaact tgtgcttttc ctccaaacat atatctcatt
     3841 tttgccgctt gatttacaat tgcatccata gaaattgtta caaagtccat aaacattact
     3901 tccataatag gtcttaaacc agttgctgct gcacctgctg ccgctcctgc tatagctgct
     3961 tctgatatag gagtatccct tactctttct ggtccaaatt catctatcat accaactgat
     4021 acaccaaatg ctcctccata tattcctata tcttctccca taaatattac attttcatct
     4081 cttctcattt cttctgacat tgcttccttt attgcttgtg catatgttaa ttcccttgta
     4141 ctcattttta ttttcctcct ttaccattct tagtctgcat atacatcctc taataaagac
     4201 tctatttttg gatttgggct attttgagca aactctactg catcttctat tgattgcttt
     4261 gcaaattctt gaatcttatc taattcatct tcattagata gattattttc tataagataa
     4321 ttttttaaaa attcaatagg gtcttttgct ttccatgatt ctatttcttc ttttgttcta
     4381 tatacatttg catctgattt tgaatgacct aaccatctat aagttctact ttcaattaat
     4441 acaggtcctt ctcctcttct acacttctca gcagcttttt gtactgtttc gtatacttct
     4501 attgggttat atccatctat tgatattcct tctattccat aagaagctgc tctagtggca
     4561 atactttcta tattcatatg acgttttatg cttgttgaca taccgtataa attattctca
     4621 cagtaaaata ttattggcaa tttccatatt gaagataaat ttattccttc atgaaaagta
     4681 ccttcatttg atgctccatc accaaaacta cataaaacta ttttgcctgt ttttttatac
     4741 tgttgtgtaa gtgcagctcc tggagctatt gtaagacctc cacctacaac tccatttgct
     4801 cctaagttac ctgactcaat atctgctata tgcattgagc ctccttttcc cttacaaaat
     4861 ccagtttctt tacccataag ttcagccatc attttattta ggtctattcc cattcctata
     4921 aattgactat gtcctctatg agttaatgat actaaatctc ctttttctaa agccattaca
     4981 gccgccacac ttgacgcttc ttgacctaca gataagtgag ttgttccatg aaccatacct
     5041 cttgcaaaaa accaactaac tttctcttca aattttctag cctgattcat tctcttatac
     5101 atttcaagca atgtttcttt tgatattgac atatacattc cctcctgtta acttttatct
     5161 atctttctat tagtcacaat cttctaataa tttaagtgct aaactgtgat taattgctaa
     5221 actatttata aatttcgctt ttaatgatgc ctttattgcc tctatttttt catctcctga
     5281 agcgacacct attgttcttt ttatcttttt taaatcttcc agtttcactc ctaatactct
     5341 ttgattaaat tcaagtatag aagtttttcc ttctttatca aaggcttgta aacaaattgc
     5401 acctattgag ttgtattttt ttaaatcttc tatatctgct tcattcatgt atccagaagc
     5461 cattattgtt gagtttaaac tcataggatt tcctataccc acaattgcta tagttgtttt
     5521 atctattaaa tctagtactt ctttaatttt ttcgtctttg caaagtttct cttttgtaga
     5581 caagtcagac ataacagctg gagcatgtaa taatttaaaa tcacctccaa aagccctcgc
     5641 taaacttact gctatttgat ttgcatgaat atctatttca ttgtctccaa tccctcctaa
     5701 tagaggtata aatgtaacat ttttacagtt acttttttcc acatatcttg gtatttcttt
     5761 tagtgttgtt cctattgaaa cacctattat atcattttcc tttactactc tagttaaata
     5821 ttcagataca gctcttgcta gttcttgttt ttgtgttagt gcatcctgtt tatcatctac
     5881 tactattact tcttttaatt tatattttct ctccaaactt ctctctattt tttggtaatc
     5941 atttttcaaa acatcaacta tctgtatttt tactattcct tgttcaaaag cttcttttaa
     6001 tattcttgat attgttggtc ttgatattcc caattgattt gctatttcct gttgattcaa
     6061 attatcttcg taataaagtg aacaacactt catcattaat cttaagtctc caattttttt
     6121 catttgcttt cctccaaaat caagcttttc aattatcttt acatttgttc aatcatttta
     6181 cttaatttca gtaacattat agaaggggtt catataaaaa gtcaactgtt tttttgtata
     6241 acaaaaaaaa ggtgatactt ttcttgtacc accaccttca taaatagttg aaaaattgct
     6301 tcttagtgca tattatatat tacttctaat tgttaaatat catctccata aattaaaaat
     6361 taattgtcca cttataatgc tttaaataac aataacttat taaatcattt ctaaaaaacc
     6421 aaccatttca tcatttaaag tcttcttata tttttattat tcgcttctct aaaaattctt
     6481 caataatcat aaatatataa tagtttttat agggaacatt tacaatagac cttaattaaa
     6541 cttaagactt tataagaaac tttttcatta tttagtattt aattcatcaa gtatagtact
     6601 atattcttta tcaagcttat aaaaagacat tatgataatt tgtaatattg ttataaatat
     6661 aggtaggtat ataaaacata cttttattga tactatagct aaatcacttt gagtagctga
     6721 tgttcctaca taaccaccaa ttgataatac tgcacctaat attgctgaac ctaatccatt
     6781 tcccacttta aacccaaaac tagatgcact atttataagc ccctcagttc tatatccagt
     6841 cttccattct ccatattcaa tagtatctga aacaatagca aacattgttg atgctattcc
     6901 agcacttcct attcccttta tagcacttgc aaatattact cctatatgat tttgtccaac
     6961 tattataaac ataacatttg acgatattaa tattattgaa cctactatta atacatttct
     7021 ttttccaaat ttttttataa aaggagcaac cataaacata gctataattt gggatatatt
     7081 tgaaaccata tttataggac caaataattt ttcatctccc aaaataaact ttgcataata
     7141 tacggatgaa ccaccattta tcgcaaatac aataaatatg caaactaaac ataatgttat
     7201 ttgtatccag tacttatttc taaataaaga tttaatacct attttaaatg gtattacttc
     7261 attcctatct tgcttaactg cttttactct ttccttggtt ccagtaaatg ttataaaaaa
     7321 taccattata gatgctattc caaatacaaa gaatgtttta gtccatgctg ataaattatt
     7381 tccaaagtat tcaactagag gtagtgtaag tccatttatt agtaaggtac cacaagtggc
     7441 taatatcatt ctaaatatac ttaatactcc tctttcatac tggttttgag ttataagtga
     7501 atttagagtt gcatagggta cattgattgc tgtataaata acagtagaaa ctaagttata
     7561 tgtaataaaa atataaatta atttagaagt gataccaaga tttgatggaa cagaaaataa
     7621 taatactgct gctatagcaa aaggaatcgc cattctcaaa atccaaggcc tagccttacc
     7681 gtatttggat ttagttctat ccactataat tcccattgtt aagtcactaa acccatccag
     7741 tatcctagat acaagcataa tactacctac agctgctgca ctaacatctg cataattagt
     7801 ataataaaac agcaaaaatg ctgacatagc tgagtatatg atattgcacc ccaaatcccc
     7861 acaaccataa gaaattcttt caaaaaatga aatcttttca tatctaatcg tagttgcttt
     7921 aacattaact tctgtattca taataccata ctcctttttg taagatttgc cttctaatgt
     7981 ttcttatttc agagcataag atttaccttt gctctgaaaa ttttagtaat gattttatta
     8041 atcgacatct tttgattcat ctactatata ttcaaaaaaa tcaaaatcag catagtgttt
     8101 atgctttaca cgatctgcac aagttaatcc tataaacgta cctgtaaatt ctccatattt
     8161 acagtactca tctgaaaact tagttgtatc aaataccttt cctattctct ggtagttttt
     8221 ttcatcataa ctccattcaa aatatgactt acgaccttgt acatacagac gaagatatat
     8281 tggtatatct tttattggta tcctagtatt taaaaattcc gttttctctc cattttcaag
     8341 atgtattatt gatagtgcac tttgacccaa tgtttcacta taatactttc ttaaatttat
     8401 ataattcata ttatcataat acattatcaa acccgcacta tgctgatgta cctctgggta
     8461 aaactccatc ttagtggtta ttcttgcata tactgaagtt aattttcttg ccaatatgct
     8521 cactttattt aatgatgttc ttgattcttg tcctctaatc cttacatatc ctggtctaga
     8581 cttaacatct gcaaatgata atggtgaaat cctaggagca tagtactgta aacctaattc
     8641 atttgaatca aagtcatcaa aacttggtat agatgaaact aaatgttctt ctagtttact
     8701 ttctgatacg tattcttttg ccaaattact ttcatcttcc attcgaagcc aattatcctt
     8761 tgtccatttc attttttgaa tagctgtttc tctacctaaa gtacacctta actctggtac
     8821 aaatggtcta gatgttagat gaactaaata tacttcacct gatgttgttt ctatatagct
     8881 tccatgacca gatttttgaa gttttgattc tggattatag tatttaggct ttagatggtc
     8941 agggtcatgt ctttcataaa aatcgcctgg aattgaagtt acaattggat tcattgaatc
     9001 tttttcataa ggtccccata tatttttagc ccttcccata gttacactat gaccatatcc
     9061 agttccacct tctgcacaca ttatataata gtaatctcca cgctttgtta tatgtggtgc
     9121 ttcaatacaa cctctatcag ttccccctga ccatattctc tttggatatc ctactatttc
     9181 tttctttttt gtacagtatt ccactaaaca tataacacca ggtttttcat atccttctct
     9241 agtctcccag tctaatgatg caatccactt tttaccatca tcatcatgaa aaatagacgc
     9301 atcaaaacct gatgagtgta gatatactgg ttcactccac tctcccttta tatcttttga
     9361 tgtaattaaa taattatcaa catcaaaata tcttgcattc atagagttca taattccata
     9421 aactatgtaa aataaatctt cttcttcaca ataagtcaaa cagggtgccc aaattccttt
     9481 agatgaagga agcttcttca aatctatttt tgtttcatct gtcaaaatat gagtatataa
     9541 ctcccaattt tttaagtctt tagagtgata aacaggtata cctggaaacc attcaaaaga
     9601 tgaaactaca agataataat catctccctt tctacaaata caaggatctg gattaaaacc
     9661 tggaagtata gggtttttaa tcatatcttt tcctccaatt ttagtttaat attctatagg
     9721 ataccattct tgagctacac atttgcctag gtcccaacta tcccatccta tccaaccttc
     9781 ttcattaact gtatctagta ataacttata attccaatag tagtgaccac ttccatttct
     9841 ccatgcatca agctgtgctt ttgctatctt cctatataat tctcttttat cgtcttttga
     9901 taatttatct atatttgatt caatcccatt tagaggtgat tgacctccat ttgtatctat
     9961 accacaaaca tatgaattaa ataagctcca ctccccacat ataacaggaa aatatttttg
    10021 catttgtaaa atatctttag cataattttc tctaatatat ttgagatagc tatcaatact
    10081 ttgttcacat ccatctgctt cagctaacat caaatattgg tgtgtatcta gaacaacatt
    10141 tttaaattct tcctctctca taaagtcttt ccatgctttt aattcaaatg catcatgaaa
    10201 cactataacg ttttcctctc tcatataagg acgcattacc ctatatgctt ttatataaaa
    10261 atcacgtaaa aactctaatg aattaggttt actccccctt gccatctcct tatctacagc
    10321 cttaaaccga tttggaacat tcattatgtt ccacattctt tctgttatag gctcatttaa
    10381 aatttggatt ccatacaatt catgcctcat cccataacgt tttgcaagtc tttctaatac
    10441 atttaatgta aacgctacat attctggatt ttgtgaccag ctacatatac ctgatattcc
    10501 tccattatca aatccatttt ggcttcctgg aactgtatgt aagtctatca atatactaag
    10561 cccatactta tctgcccaag caaatgcttt atcaagttct ttaacacatc ctatgaatgg
    10621 ttcacaatct ccaaaaataa agtatggtac tggtatacga actgaattaa atcccataga
    10681 tttaatcgtt gcaaaatctc tctctgtgat gtattcacta cgatgagtct taattctaga
    10741 ttcatatgcc tctcttgaaa gctgtcttgg taaatagtat tcatcttcag cttctgtgcc
    10801 ttcaaataaa cttgaggtca tccatttttc taaaactaac caacctccta aatttacacc
    10861 ttttatatac acataaaatc ctcccctctt atcttattta aataggtata ttttgcactt
    10921 ataataaaat aattttttta agttatttta atatacagtt attatatttg attttatccg
    10981 ttttcttaag tatgtttatt atctaattta ttagtctata ttatcttttt aatattgaac
    11041 attataaatt tactatttat aatttaaaat tataaggagg atattattat gaacagaaat
    11101 aatttttcac atgaaataat tgaacctatt gataatttag atgttaattt caaactattt
    11161 gatgatagtg gtagttatgt agcaaatcat tggcataact cattagaaat aatttatata
    11221 acttctggtg atttacaaat taacatggag ggttacactt ataatttaaa agctaatgag
    11281 tgtatgttca ttaattctgg aattattcac tcaactagat gtatttatca aaatacctct
    11341 atattattgc aagtaccatt gtcttttcta aacaaatata tccctgattt taaaaattgc
    11401 tattttgact ttaaggttaa tgccaatgac aataactata aaaaaaattc aagtaaagtt
    11461 aaacttatat tagaaaacat gagagaaatt aaacttagtt ctcctcctgc agctaatctg
    11521 ctctttacaa gcttattatt tgaactttta tttgaattat atacaaactt taaaatatct
    11581 gtaggtaata aaaatatgaa aaaaacagta cttgatttgt caaaatttga accagtttta
    11641 gaatatacaa agattaatta caaaatgcct atttctatta acaaaatcgc taaggtagca
    11701 cacttacaac cagaatattt ttgtagaaaa tttaaacagt atatgggaca aacttactta
    11761 gagtatttaa atgatgtgcg tatttctcat atttataaag atttaattaa cacaaatgat
    11821 accttatgca gtatattaga agctcatgga tttactaata caaaattatt ttacagaatt
    11881 tttaaggaaa aatttaaatg cactcctaaa catataagaa aaaaatctac ctaaaatatg
    11941 aggttatatg ttatttatct ttataataaa aaaagtttat cttaaaattt gattaga
//
LOCUS       DBGJFF010000118        11762 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.120, whole genome shotgun sequence.
ACCESSION   DBGJFF010000118 DBGJFF010000000
VERSION     DBGJFF010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11762
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.120"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            97..258
                     /locus_tag="ACOIAF_003038"
     CDS             97..258
                     /locus_tag="ACOIAF_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III"
                     /protein_id="HGZ2670039.1"
                     /translation="MKQDKQNEKQSEISKIKQSNDNKLEIAQELHVSKNIGNMLGYRI
                     DKKKMNRPS"
     gene            599..1867
                     /locus_tag="ACOIAF_003039"
     CDS             599..1867
                     /locus_tag="ACOIAF_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HGZ2670040.1"
                     /translation="MEASIHTIASNNLLIIFAIVSITGIICSKLSEIIKVPDVVLYLL
                     VGILIGPSFLKFIDIRGFQIENQLILTFGSAFILYLGGKEISLKVLRNVKISVFLLAT
                     LGVVISAFIMQQVIGFTFGISAMTALLAGTIIASTDPATLVPIFNQVKIKDRVKQTVI
                     SESAFNDATGAILTSAVLAVILSNKFSLGENIYELGTMVIVGVLVGLITGILLLKLVN
                     DKPYGIFKDFAPIISVISVVVAYELSTKLGGSGYMSCFIVGIITGNKKNFKIWLSQKS
                     YDADFYVAETLGTICRMAIFIILGSQVNLAQLSKYFMPSVITVLVLMFIARPLCVLVC
                     TLVDREAKWSKSEILFMMWVRETGVIPAALCGIISAMKVPGYEVISSVVFMTILITLV
                     IQGSTTKLVAKKLGLLEEEVINISEKVSTF"
     gene            2062..2850
                     /locus_tag="ACOIAF_003040"
     CDS             2062..2850
                     /locus_tag="ACOIAF_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HGZ2670041.1"
                     /translation="MFTVMDIVQPDTVEEAYSILNKRKTNQVIGGSAFLRMGKKRIGT
                     GIELSKLNLDYIKEYEDYVEIGSMTTFRTLETSSIIKNNFGRIIEDSVKNIIGVQFRN
                     VVTVGATVFSKYGFSDLIVALLSLDTEVELYNIGRISLEEFLNRDYEKDLLIKIYIKK
                     TNKNASYKSLRNAKSDYPILNVSVSKHMEQFKLCVGARPQKATIAKQASEFLSNNEIN
                     EINIDKAVEIASEELTFGSNMRASREYRKAMSKVLLKRAIMEVI"
     gene            2850..3299
                     /locus_tag="ACOIAF_003041"
     CDS             2850..3299
                     /locus_tag="ACOIAF_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HGZ2670042.1"
                     /translation="MLVKININGKKKSVDIEPEEYLVDTLRKVGNLSVKRGCDTGCCG
                     LCTVLIDKKPTLSCATLTLRALDKEITTIEGLENEVKEFSEVLVKEGAEQCGFCSPGF
                     ILTVLAMKDELENPTDDEIKHYLTGNLCRCTGYMGQLRAIKTYLEVK"
     gene            3303..5567
                     /locus_tag="ACOIAF_003042"
     CDS             3303..5567
                     /locus_tag="ACOIAF_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HGZ2670043.1"
                     /translation="MNIVGKGIKKIDGMSITTGKPVYTDDLSAKDALVVKILRSPHAY
                     AKIKDIETSKAEMVDGVECVLTYKDVPKSRFTLAGQTYPEPSPYDRLILEDVVRYVGD
                     EVAIVAAIDEKTAIKAMNLIKVKYEVLTPVLDFEEAIGNKTMVHDEDRHCNFDIGMQR
                     EKNIVSHHDYTKGDVEKVLSNCDVVIEETYYTQAQAHAMMETYRAYNYLDHMGRLVVV
                     SSTQIPFHVKRHLSRALEIPASKIRVIKPRIGGGFGGKQTACVEIFSAIVTLKTGKPA
                     KIIYDRKETFNCSTSRHAMRLKVRLGATNDGIIKAIDIKALSDTGAYGEHASTTIGLV
                     GEKTLPMYNKVEATRFIADVVYTNKMPAGALRGYGASQGCFAVESTINMLANKLKMDP
                     TDLRLKNVVTEGETTFAYGKTLNSVDLIKCINKGKELIGWNEKYPCKHLDNGKIRSVG
                     MAITMQGSGIQGLDKSSAEIKLNDCGDYTLMIGSTDMGTGSDTILAQMAAEVLDTSIE
                     NITVISADTDLVPYDPGSYASSTTYVTGMAVVKAAQDLREKIISAGATKLGVEKDEVE
                     FDGIKLTSKKGSTLLFDIAVDSTIGPDTNQLIGLASHGSPVSPPPFIAGFAETEIDTE
                     TGKVDLVNYVAVVDCGTVINKNLAKIQVEGGIVQGIGMALFEEVRYTEKGSMDTNTFM
                     QYKIPARCDIGNVKVDFVETYEPTGPFGAKSVGEVVINTPLPAIQEAVFNGIGVRINT
                     LPITPEKVFMEMNK"
     gene            5707..6018
                     /locus_tag="ACOIAF_003043"
     CDS             5707..6018
                     /locus_tag="ACOIAF_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670044.1"
                     /translation="MAGNLNNMRAVNNFRGDKNILECLVSFEGRSISQRKVRVFFKEK
                     QNQIEIDFAEEEISKLVENVVLNTSYQEMLYDEIEKQLEIDCIGTWMILSKLKDGSRV
                     H"
     gene            6304..6804
                     /locus_tag="ACOIAF_003044"
     CDS             6304..6804
                     /locus_tag="ACOIAF_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HGZ2670045.1"
                     /translation="MRKNSINIALTAAITAIYAVLTLSLGFISYGPIQFRVAEIMMLL
                     AFLDKGYIVGLTLGCFLANVIGPYGVPDIIFGTLATFISASMIYITRKLGKQNKSTLV
                     IASLWPTVINSIVVGLMLNVFFGLPLILSMIQVGFGEFVVVTIVGVPFFCFLMKKYKN
                     VINIKF"
     gene            6861..7847
                     /locus_tag="ACOIAF_003045"
     CDS             6861..7847
                     /locus_tag="ACOIAF_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGZ2670046.1"
                     /translation="MSKVKRLTDVWIYINDKQKFTIKELSEEFNLSAKTIQRYLKELN
                     KMGLPIQAEQGRNGGYRVLNNSYIPPVIFTEKEVMIILFALKSLQIYNYKLMEIEINS
                     IMRKVSYERKSSIKVGIENIKRYIGFVANDTNDGDFKSSNVTEFFRASSESLILNIKY
                     KCGNQILEKNVCPIGIYLNKGAWFSPAYDCKSDKIILVHIDNIIDIKRTGESKKIHIN
                     LEEWLHEVYNNLYGNSSTTKKFGEIIYLNVLLTKEGVSKIKDSSWNLDMELNEDGSGI
                     ISTFIKVEDIDCYASILFFIGNNAKVIEPKFLNKLLYDKANELRYFYEENMV"
     gene            7897..8619
                     /locus_tag="ACOIAF_003046"
     CDS             7897..8619
                     /locus_tag="ACOIAF_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein"
                     /protein_id="HGZ2670047.1"
                     /translation="MENFNKNAKIIGIGAEGINIINEVEEKIKANMDIEKININQEIE
                     KEYVRSLLDGVDILFLIYSSEDKHIRDIIKAVSYMSNERRVLSIGMDCSEKENKEDLE
                     LGREFKINNDSIFKFVDLMNIMVESISDSCMINIDITDLKEAIVGDKGIKYSFEEFED
                     TKSYSEIADILFDRMEYIGEEFISKKGIVFVEGSSEFSIMELNDLISNIQSKVEESYE
                     VIFSLYIKENLNGNIRVGLLYN"
     gene            8648..10582
                     /locus_tag="ACOIAF_003047"
     CDS             8648..10582
                     /locus_tag="ACOIAF_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HGZ2670048.1"
                     /translation="MSENIRGTEYWLMEEFFYQETDTPQIFREESIIALGFDIKINLK
                     KVFSMGKKSEKYINENCNDKDFIKKFMSIKKGDYFLYKAYQNIEEKVKCNVSIGIVEE
                     DFENGYELSHILGHTLPVKWMHNFDTVSNCKVYTSEFYKLNNNDISSYLEIIKSQDKK
                     LLRKRIEESENNKWYLKNYYYKNVDKCVDDEELFIQPINLEQLESLKNIKTDDYILLY
                     NSEKVICENDHTMKSLFGVCQVMEGFDKEKVSALEGGYFFPVRWIEYEKENIDGNAWV
                     IFENNSIVEKYVKRFTNKMPLNTILYGPPGTGKTYNTKQMVYNLVSETSLRNRYYTGY
                     LDYKNNVEFCTFHQSYGYEEFIEGLKSDGEGNFKPEDGILKDISIEACYNALKFEKKM
                     SFELEKDSLTLETIKDRKKKLVLENIEYSESFNFEESENYVLIIDEINRGNVSKIFGE
                     LITLLEDDKRLTKSNQTIVKLPYSKEKFSLPPNLYIVGTMNTSDKSIALLDIALRRRF
                     NFEEIMPDYDLLSIVDGIDIRKMLYTINKRIEFLYDRDHVIGQAYLLAVENINDIVVV
                     FRSKIIPLLQEYFYYDSEKVGLILGGIGRNEKDKYIVYKEKLIASELFKNNNASIMES
                     KVNYCIKQNISSEELKNIYE"
     gene            10575..>11762
                     /locus_tag="ACOIAF_003048"
     CDS             10575..>11762
                     /locus_tag="ACOIAF_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HGZ2670049.1"
                     /translation="MSKNIYVKETYEWIRIGNGENELTEIEYEKLLKYLENNNDVLKS
                     NIIDIKYKKLRFINYVGIICFENVILEILPKLSLSDNLVKDREILLQMLSICNKIPIT
                     MNEKIRLSLKNYNLLNFFVMYFIESMQTQMKKGIYFEYINKIENLNVMRGKILLSTYA
                     KEKGISPMKIRCEYDEYSENNFLNQVLKKACISILCRINDNSIQGKIKKILSYFQNVD
                     LIHIDRKKLLDYKFYKNNDRFKDCYLLARLLLLNLSMDNSQNNQEAFSILFEINTLYE
                     EYIGILIKSIWDNSFRETYIQDKSKFLLKNEQTGKKNFNLRPDIVLYDLKNEYEIIID
                     TKWKAIEVDSNVFYRSSDIYQMYAYITAYENAKRCILLYPCIQKDKNYSSWKLSESFK
                     GKFI"
BASE COUNT         4735 a         1067 c         2118 g         3842 t
ORIGIN      
        1 tatattgaga tatttccctt ttttgattaa aattatagaa atttgaaata ataagtaata
       61 aacagaaaaa gtgatattgt tttgaaagga gaaactatga aacaagataa acaaaatgaa
      121 aaacaaagtg agatttctaa gataaaacaa agcaatgata ataaacttga aatagctcaa
      181 gaattacatg taagtaaaaa tatagggaat atgcttggat atagaataga taagaaaaaa
      241 atgaatagac caagttaatg aaaatgttga aaatggtaca ttttaaaaaa tatatataat
      301 gaaatgtttt attgttaaat ttaaacttat tttaatgggg gaaaaagtcg aaaaatatct
      361 aaaaagatat ggatataaaa aatattaata tatatatact ataaacttga caaaatcaac
      421 cttttaataa ctagcaaaaa atagcaaaat tatataagcg ttatttaatt gtcaaaaatg
      481 aaagctaaat tgcatgtcct aaaaaaaatg attgataaca tttatttgtg tgctataatt
      541 atatagtaaa acaataagta tattgcataa tgtatgcaaa tcgggaggag agacggacat
      601 ggaagcatct atacacacaa ttgccagcaa taatttattg ataatttttg ctattgtaag
      661 tataactgga ataatttgta gtaagcttag tgaaataata aaggttccag atgtagtgtt
      721 atatttactt gtaggtattt taataggacc atcattttta aaatttatag atattcgtgg
      781 atttcaaata gaaaatcaac taatacttac atttggttct gcatttattt tatatttagg
      841 tggaaaagaa ataagtttaa aagtattgag aaatgtgaag atatctgtat tcttattagc
      901 tactttagga gttgtgatat ctgcttttat aatgcaacaa gtaataggat ttacttttgg
      961 gataagtgca atgactgctt tactagcagg aactataata gcatcaacag acccagccac
     1021 tcttgtacct atatttaatc aggtaaagat aaaggatagg gtaaaacaaa ctgtaataag
     1081 tgaatcagct tttaatgatg caacaggagc tatactaaca tcagcagtgt tggctgtaat
     1141 tttgtcaaat aaattttcat taggtgaaaa tatttatgaa ctggggacaa tggttattgt
     1201 cggagtttta gttggtttaa ttacaggaat actgcttttg aaattagtta atgataaacc
     1261 ttatggaata tttaaagatt ttgcaccaat aatatctgtt atttcagttg tagttgcata
     1321 tgaattatct acaaaattag gtggaagtgg atatatgtct tgctttattg taggtataat
     1381 aactggtaac aagaaaaatt ttaaaatttg gttaagtcaa aaatcttatg atgcagattt
     1441 ttatgttgct gaaactttag gtactatttg tcgtatggca atatttataa tattgggtag
     1501 ccaagttaat ttggctcagt taagtaaata ttttatgcca tctgtaatta cagtattagt
     1561 attaatgttt atagcaagac cactttgcgt tttagtttgt acacttgtag acagggaagc
     1621 aaaatggagt aaaagtgaaa tattatttat gatgtgggta agggaaacag gcgtaatacc
     1681 agcagcactt tgtggtatta tttcagcaat gaaagttcct ggatacgaag tgatttcatc
     1741 tgtagtattc atgactatac taattacatt agtaatacaa ggaagtacta caaaattagt
     1801 agctaaaaaa cttggattat tagaagaaga agtgataaat ataagtgaaa aagtatcaac
     1861 tttttaaagt tttgctttaa aatagtacct gttaatggct attttaatat tgtctaatga
     1921 tgattattga aatagtcatt tttttatgta taagtaattt acagaattta catattagac
     1981 atatttatta tcaaaatttg ttgaagctat gatataatta gttgtattta aaataagaat
     2041 agcaaataag ggaggaaatt tatgtttacg gttatggata ttgtacagcc agatacagta
     2101 gaagaagcgt actcaatttt aaataaaaga aaaactaacc aagtaattgg tggaagtgct
     2161 tttttaagaa tggggaaaaa aagaatagga acaggaatag aattatctaa attaaattta
     2221 gattatataa aagagtatga agattacgtt gagattggtt ctatgactac ttttagaaca
     2281 ttagaaacaa gttccataat aaaaaataat tttggaagaa taatagaaga ttcagttaaa
     2341 aatataatag gagttcagtt tagaaatgta gtaacagttg gtgcaacagt attttctaaa
     2401 tatggttttt ctgacttgat agtagcatta ctttcattag atacagaagt agaattatat
     2461 aatattggga gaattagtct tgaagagttt ctaaatagag attatgagaa agatttactt
     2521 attaagatat acataaagaa gacaaataag aatgcttctt ataaatcact tagaaatgca
     2581 aagagtgatt atccaattct aaatgtatct gtatcaaaac atatggaaca atttaagtta
     2641 tgtgtaggag caagaccgca gaaagctacg attgcaaaac aagctagtga atttctatct
     2701 aacaatgaaa tcaatgaaat taatatagat aaagcagtag aaatagcatc agaagaacta
     2761 acttttggtt ctaacatgag agcatctagg gagtatagaa aagctatgag taaagtactt
     2821 ttaaaaagag ctataatgga ggttatttaa tgttagtaaa gattaatata aatgggaaga
     2881 aaaaaagtgt tgatatagaa ccagaagaat atctggttga tactttaaga aaagttggta
     2941 atttaagtgt gaaaagaggt tgtgacacag gctgttgtgg tttatgtaca gttttaattg
     3001 acaagaaacc tacattatcc tgtgcaacac tgacattgag agctttagat aaagaaataa
     3061 ctacaattga aggtttagaa aatgaagtta aagaatttag tgaagttttg gtaaaagaag
     3121 gagctgagca atgtggtttc tgttctcctg gctttatact tactgtactt gcaatgaagg
     3181 atgagcttga aaatcctaca gatgatgaaa taaagcatta tcttacagga aatctatgta
     3241 gatgtacagg gtatatggga cagttaagag ctatcaaaac ttatctggag gtaaaataga
     3301 acatgaatat agttggaaaa ggaatcaaga aaatagatgg aatgagtatc acaacaggaa
     3361 agcctgtata tacagatgat ttatcagcaa aagatgcttt ggtggtaaag atacttagaa
     3421 gtcctcacgc ttatgcaaaa ataaaagata tagagacatc aaaggcagaa atggttgatg
     3481 gtgttgaatg tgttttaaca tataaagatg taccaaaatc tcgatttaca cttgctggtc
     3541 aaacatatcc agagccatct ccatatgata gattaattct tgaagatgtt gtaagatatg
     3601 taggtgacga agtagctata gtggcagcta tagatgaaaa aactgcaatt aaagcaatga
     3661 atcttatcaa agtaaagtat gaagtactta cacctgtttt ggattttgaa gaggcaatag
     3721 gaaataaaac tatggtacat gatgaagata gacattgtaa ctttgatata ggtatgcaaa
     3781 gagaaaaaaa tatagtatcc catcatgatt atacaaaagg tgatgtagaa aaagtattaa
     3841 gtaattgtga tgtagtgatt gaagaaactt attatactca agctcaagca catgcaatga
     3901 tggagacgta tagggcatac aattatttag accatatggg aagattggtt gtagtaagtt
     3961 caactcaaat accatttcat gtaaaaagac atttatctcg tgcactagaa ataccagcta
     4021 gcaaaattag ggttataaaa cctagaattg gtggcggatt tggtggtaaa caaactgcat
     4081 gtgttgagat tttttctgca attgttactt taaaaacagg gaaaccagct aaaataattt
     4141 atgatagaaa agaaactttt aattgttcaa caagtagaca tgcaatgaga ttaaaagtta
     4201 gactaggtgc tacaaatgat ggcataataa aggcaataga tataaaagca ctttctgata
     4261 caggagcata tggagagcat gcttctacaa ctattggttt ggttggagaa aaaactttac
     4321 caatgtataa taaagttgaa gcaacaagat ttattgcaga tgtggtatat actaataaga
     4381 tgccagcagg agcacttaga gggtatggag catcacaagg atgttttgct gttgaatcaa
     4441 ctataaatat gcttgctaat aaattaaaaa tggacccaac agatttaagg ttaaaaaatg
     4501 tggtaacaga aggtgaaact acatttgcat atggtaaaac tttaaatagt gtcgatttaa
     4561 taaaatgtat aaataaaggc aaagaactta ttggatggaa tgaaaaatat ccttgtaaac
     4621 atcttgataa tggaaaaatt agaagtgtgg gtatggcaat tactatgcaa ggttctggta
     4681 tacaaggtct tgataagtct tcagctgaaa taaaattgaa tgactgtgga gattatactc
     4741 ttatgatagg ttctactgat atgggtacag gaagtgatac gattttagct caaatggctg
     4801 ctgaggttct tgatacaagc attgaaaata taacagttat atcagctgat actgacttag
     4861 ttccatatga cccaggttca tatgcttcaa gtactactta tgtaacaggt atggcagtag
     4921 ttaaggcagc tcaagactta agagaaaaaa taatttctgc tggagcaact aaactaggtg
     4981 ttgagaagga tgaagtagaa tttgatggta taaaattaac tagtaaaaaa ggtagcactt
     5041 tattattcga tatagcagtt gattctacaa ttggtccaga tactaatcaa ttaataggtc
     5101 ttgcttctca tggtagccca gtatctcctc ctccttttat agcaggtttt gcagaaactg
     5161 aaattgacac agaaactgga aaagttgatt tagtaaatta tgtagctgta gtagattgtg
     5221 gaacggttat aaataaaaat ctagctaaga tacaagttga aggtggaatt gttcaaggga
     5281 ttggaatggc attatttgaa gaagttagat acacagaaaa aggaagcatg gacacaaata
     5341 catttatgca atacaagatt ccagctagat gtgatattgg aaatgttaaa gtagattttg
     5401 ttgaaactta tgaaccaact ggaccatttg gtgctaaatc tgttggagaa gttgttataa
     5461 atacaccttt accagcaata caagaagctg tgtttaatgg aattggtgta agaataaata
     5521 cactccctat tacacctgaa aaagttttta tggaaatgaa taaataatta tttatgaaaa
     5581 tttagactag ttttacatgt taaacaacca gttatgtcat acctgttgaa agatatttaa
     5641 taagtagtta ttataatatt atgaatttta cttataaaat acaaaattaa ataaggggat
     5701 gatgttttgg ctggtaatct aaataatatg agagcagtaa ataattttag aggagataag
     5761 aacattttag aatgtttagt cagctttgag ggtcgttcaa taagtcagag aaaagtaagg
     5821 gtatttttta aagaaaaaca aaatcaaata gaaattgatt ttgcagaaga ggaaatttct
     5881 aaattggttg aaaatgttgt tttaaataca tcatatcaag aaatgttata tgatgaaata
     5941 gagaaacaac tggaaattga ttgtataggt acttggatga tattatctaa attaaaagat
     6001 ggtagtagag ttcactgatt ttaaaataaa ttattagagc ctaattttaa ggctcttttt
     6061 tgctgttttt aattaacaat agaaaattta tctttagttt gtatataatt ttaatatcat
     6121 atattctata tatataaaat tgaatttgta tgtattatgt tgtataatat aaaatgaatt
     6181 aggaaaatag ttcgctaaaa taatttgttt ttataaaatt actgtttaag tttatagatt
     6241 ttgtataaaa taaagttatg ttattaattt ttttagaaat aaaactttta ctaggagaca
     6301 gatatgagaa aaaatagtat taatatagca ctaactgcag caattacagc aatctatgca
     6361 gttttaacat tatcattggg atttataagt tatggaccaa tacaatttag ggtagcagag
     6421 attatgatgc ttttggcatt tttagataaa ggatatatag taggtcttac attaggttgt
     6481 tttttagcaa atgtaatagg accatatggt gtacctgata taatttttgg aacattagcc
     6541 acatttataa gtgcaagtat gatttatatt actagaaagt taggtaaaca gaataaaagt
     6601 actttagtta tagcttcttt atggccaact gttataaatt cgattgtggt tggtctaatg
     6661 ttaaatgtat tctttgggct tccattaatt ctatctatga tacaagtagg ttttggagaa
     6721 tttgtagtag taacaattgt aggagtacca tttttctgct ttttaatgaa aaaatataaa
     6781 aatgtcatta atattaaatt ttaaatagtg tatagtaatt taattaattg agagtattat
     6841 tagtgaaaag aggtgtttat ttgtcgaagg taaaaagatt gactgatgtt tggatatata
     6901 ttaatgataa gcaaaagttt acaataaaag agttgtcaga ggaatttaat ctttcagcta
     6961 aaacaataca acgatattta aaagaattaa ataaaatggg acttccaatt caagcagaac
     7021 aaggtagaaa tggaggttat agggtgctaa ataatagcta tatacctcct gttatattta
     7081 cagaaaaaga agttatgatt atactatttg cacttaagtc acttcagatt tataattata
     7141 agttaatgga aatagaaatt aattctataa tgagaaaagt aagttatgaa cgtaaaagca
     7201 gtataaaagt aggtatagaa aatattaaaa gatacattgg ctttgtagct aatgatacta
     7261 atgatggaga ttttaagagt agtaatgtaa ctgagttttt tagagcttct tcagaatctc
     7321 taattttaaa tattaagtat aagtgtggta atcagatttt ggaaaaaaac gtatgcccta
     7381 taggtatata tttaaataag ggagcatggt tttcaccagc atatgattgt aaaagtgata
     7441 aaataatatt agttcatata gataatataa ttgatataaa aagaacagga gaaagtaaaa
     7501 aaatacatat aaacttggaa gaatggttac atgaagtata taacaattta tatggaaata
     7561 gttcaactac taaaaaattt ggtgaaatta tttatctaaa tgtattatta actaaagaag
     7621 gtgtttctaa aataaaggac agttcttgga atttagatat ggagctaaat gaagatggta
     7681 gtggaataat tagtacattc ataaaggttg aagatataga ttgttatgct tcgattttat
     7741 tttttattgg aaataatgct aaggttatag agccaaaatt tttgaataag ctactttatg
     7801 ataaggccaa tgagttaaga tatttttatg aagaaaatat ggtataatat ttagaaatgt
     7861 tgaaattaaa taattattaa ggagatatac gttataatgg aaaattttaa taagaatgct
     7921 aaaataattg gtattggagc tgaaggtatt aatataataa atgaagtaga agaaaaaata
     7981 aaagcaaata tggatataga aaaaataaac ataaatcaag agattgaaaa agaatatgtt
     8041 agaagtcttt tagatggtgt agatatatta tttttgatat attcatctga agataaacat
     8101 attagagata ttataaaagc tgtttcatat atgtcaaacg aaagaagagt tctatctata
     8161 ggaatggatt gttcagagaa ggaaaataaa gaagatttag agcttgggag agagtttaaa
     8221 atcaataatg atagtatatt taaatttgtt gatttaatga atatcatggt agagtctatt
     8281 tctgattctt gcatgataaa tatagatatt actgatttaa aagaagctat tgtaggagat
     8341 aaaggaatta agtattcttt tgaagagttt gaagatacaa agtcatatag cgagatagct
     8401 gacattttat ttgacaggat ggaatatatt ggtgaggaat ttataagtaa gaaaggaatt
     8461 gtatttgttg aaggaagttc agaattttct ataatggaat tgaatgattt aataagtaat
     8521 attcagagca aggtagaaga aagctatgaa gtaatatttt ctttatatat aaaggaaaat
     8581 ttaaatggaa atattagagt aggcttattg tataattagt atattttaga tattaaggag
     8641 agtgtatatg tctgaaaata taagaggtac agagtattgg ttaatggaag aattttttta
     8701 tcaggaaaca gacacaccac agatttttag agaagaaagc ataattgcac ttggatttga
     8761 tataaaaata aatttaaaaa aagtattcag tatggggaaa aaatcagaaa agtatattaa
     8821 tgagaattgt aatgataaag attttataaa aaagtttatg tcgattaaaa aaggagatta
     8881 ttttttatat aaagcatatc aaaatataga agaaaaagtt aaatgtaatg tgtctattgg
     8941 catagttgaa gaagattttg aaaatggata tgaattaagt catattttag ggcatacact
     9001 accagtaaaa tggatgcata attttgatac agtttcaaat tgtaaagtat atacaagtga
     9061 attttataaa ttaaataata atgatattag ctcatattta gaaattatta agtcacaaga
     9121 taagaaatta ttgagaaaaa gaatagaaga aagtgaaaat aataaatggt atttaaaaaa
     9181 ctattattat aaaaatgtag ataagtgtgt agatgatgaa gaattattta ttcagcccat
     9241 taatctagaa caattagaaa gtctaaaaaa tataaagaca gatgattata ttttattgta
     9301 taatagcgag aaagtaattt gtgaaaatga ccatacaatg aagagtctat ttggcgtatg
     9361 tcaagttatg gaagggttcg ataaagagaa agtaagtgct ttggagggtg gatatttttt
     9421 tcctgttaga tggatagagt atgaaaagga gaatatagac ggaaatgcat gggttatatt
     9481 tgaaaataat agtatagttg aaaagtatgt aaaaagattt actaataaaa tgccattaaa
     9541 tactatactt tatggaccac caggaactgg taaaacatac aatacaaaac agatggttta
     9601 caatctagta agtgaaacca gtctaagaaa cagatactat acaggttact tagattataa
     9661 aaacaatgta gaattttgta catttcatca atcatatgga tatgaggaat ttatagaggg
     9721 tcttaagtct gatggtgaag gcaattttaa gccagaagat gggatattaa aggatatatc
     9781 tatagaagca tgttataatg cattgaaatt tgaaaaaaag atgagctttg agttggaaaa
     9841 agatagcttg actttagaaa ctataaaaga caggaaaaag aaattagtat tagaaaatat
     9901 agaatatagt gagagtttta attttgaaga atcagaaaac tatgttttaa taatagatga
     9961 aattaataga gggaatgtat ctaaaatatt tggtgagcta ataacattat tggaagatga
    10021 taaaagactt actaaatcaa atcaaactat tgtcaaacta ccatattcaa aggaaaaatt
    10081 ttctctacct ccaaatttgt atatagttgg aactatgaac acatcagata aatcaatagc
    10141 acttttagat atagctttaa gaagaaggtt taattttgaa gaaattatgc cagattatga
    10201 tttattgtct attgtagatg gtatagatat tagaaaaatg ctatatacaa tcaataaaag
    10261 gatagagttt ttatatgata gagaccatgt tattggacaa gcttatctac ttgctgtaga
    10321 aaatattaat gatatagtag tagtatttag aagtaaaata ataccacttc ttcaagagta
    10381 tttttattat gattcagaaa aagtaggttt gattttagga ggaattggaa gaaatgaaaa
    10441 agataaatac attgtttata aagaaaaact tatagcttct gagttattta aaaataataa
    10501 tgcctcaata atggagagta aagttaacta ttgtataaaa caaaacatat ctagtgagga
    10561 gctaaaaaat atatatgagt aaaaatatat atgttaagga aacctatgaa tggataagga
    10621 taggtaatgg tgaaaatgag cttacagaaa ttgaatatga aaaattgctt aagtatctag
    10681 agaacaataa tgatgtatta aaaagtaata taatagatat taaatacaaa aaacttagat
    10741 ttataaacta tgtaggcatc atatgttttg aaaatgtaat acttgaaata cttcctaaat
    10801 tgtcattaag tgataatctt gtaaaagata gagagatact attacagatg ctttctatat
    10861 gtaataaaat cccaattaca atgaatgaaa aaataagatt gagtttgaaa aattataatc
    10921 tcttaaattt ttttgttatg tatttcattg agagtatgca aacacagatg aaaaaaggta
    10981 tttattttga atatataaat aaaatagaga acttaaatgt tatgaggggt aaaatattat
    11041 taagtacata tgcaaaagaa aaaggcattt ctccaatgaa aataagatgt gaatatgatg
    11101 agtatagtga aaataatttc ttgaatcaag ttcttaaaaa agcatgtata tctattttat
    11161 gtagaataaa tgacaattca attcaaggca agattaaaaa aattttaagc tattttcaaa
    11221 atgtagattt aattcatata gatagaaaaa aactgttgga ttataaattt tataaaaaca
    11281 atgatagatt taaagattgt tatttactag ctagacttct acttttaaac ttgtctatgg
    11341 ataatagtca aaataatcaa gaggcatttt ctatattatt tgaaataaat actttgtatg
    11401 aagaatatat aggaatttta atcaaaagta tatgggacaa ttcatttaga gaaacatata
    11461 tacaagataa gagtaaattt cttttgaaaa atgaacaaac aggtaagaaa aactttaatt
    11521 taagaccgga tatagtttta tatgacttaa aaaatgaata tgaaattata attgacacta
    11581 aatggaaagc tattgaagta gattcaaatg ttttttatag gtcaagtgat atataccaga
    11641 tgtatgcata tataacagca tatgaaaatg caaaaagatg tatattatta tatccttgta
    11701 ttcaaaaaga taagaattat agttcctgga aattatcaga atcatttaag ggtaagttta
    11761 ta
//
LOCUS       DBGJFF010000119        11739 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.121, whole genome shotgun sequence.
ACCESSION   DBGJFF010000119 DBGJFF010000000
VERSION     DBGJFF010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11739
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.121"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            197..1111
                     /gene="queG"
                     /locus_tag="ACOIAF_003049"
     CDS             197..1111
                     /gene="queG"
                     /locus_tag="ACOIAF_003049"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HGZ2670050.1"
                     /translation="MDNKELKAFCNLIGLNCVGVAGVDKYDNLEKILKDRQQKGYLTG
                     MEEPIIENRINPRHIMKDANSIIVCAFPYHINRQEKNKNSNLSKYCHGKDYHIVVKDF
                     LQQICDYISKTVTDFKYKLFADNGPLVDRYLAYLSGIGYFGINNNIITDEYGSYVFIG
                     YIVNNYKFEEDKPLEKSCFKCGKCVKYCPGNAILGNYDMNPKKCLSYITQKKGDLSEK
                     EKVVLKNGKKVFGCDICQEVCPHNVEIPTTHILEFKENIIDYLDYDEIQNISNKEFKR
                     RYGDRAFSWRGRNIIKRNMEIILDKYDD"
     gene            1217..3454
                     /locus_tag="ACOIAF_003050"
     CDS             1217..3454
                     /locus_tag="ACOIAF_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HGZ2670051.1"
                     /translation="MKYNNTSEINPLDYILGQERAVEAMELGLKINNPAYNIYIAGEP
                     GTGKSTYALKVLNEYASKKNTHKDWCYIYNFENPREPIIVELEKGFGRELKKDMEKLI
                     ESLLEDFKNAFESENFEIEKNKLLDEYEIEKDMLLKQIKKYGEEKGFKLKQSKMGIVF
                     IPLKEEEDESDESDEEFYKAKRELENMAIQVVYKIRNLEEIAEKAVLELEEEIAKLVV
                     EPHIKRLCEKYENYDKIQTYLENIKKDIIEYMYLFYLDEEELKDKYDKEHFIKYKINL
                     FVDNGSSKNKESAPVVVEINPSPANLFGKAEYDYANGNIKTDFTKLLSGAFHRANGGY
                     LVLYADQLLKYTMSWEILKKTLQTKKVILETQTAIKPENMPLDVKLVLIGSHYIYDIL
                     YRYDEDFEKYFKVFVDFDSEMDKNEDNEEGIARFIAYQCDKNNLRHFTKSAVEEVIKF
                     STRLSGDLEKLSTKFNKIMEVVIEGSAYAEFRNSEYTEQCDVKKAISEKRKRINRVET
                     HMDESIENGFTLIETEGRKVGVINGLSVLSTGEYSFGRASRITATTSPGSKGIVNIER
                     EVNMSGSIHNKGVLILGGYLSENFAQDLLLSLNAYVCFEQNYGGIDGDSASSAELYVL
                     LSSLSGVSIKQNIAVTGSINQKGDIQVVGGISEKIEGFYSICKTKGLNGEQGVIIPRK
                     NQRNLVLSDEVNDAVRDGKFKIYTVERVEEAIEILTDVKFEEIKKLVKEKLISFSKIQ
                     TVSKE"
     gene            3650..4702
                     /gene="nth"
                     /locus_tag="ACOIAF_003051"
     CDS             3650..4702
                     /gene="nth"
                     /locus_tag="ACOIAF_003051"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HGZ2670052.1"
                     /translation="MKNVNLKEINEKDVIVEEDINLKGMDKKDVIEKEEVNKKSIDKK
                     DIIEKEEIDKKNVKKVEVQKEIDKMIGKKDEIIDEKDIENFMDIEEDKKSIKVSKKST
                     KKDEKEKKAKKSSSSKVTQKQSKEVKSKSKIKNESANEKDVNKILDELEKLYPDAKCE
                     LNYGTAFELLIATILSAQCTDVRVNKVTSELFKKYNTARDFANLSIEEISKEIKSCGL
                     YKSKSQKIKDTSEQLCELYDGEVPDSLEKLIKLPGVGRKTAGVVLSNAFNHPAIAVDT
                     HVFRVSNRIGIVDEPNPQKTEFALMEAIPKERWSHSHHVLIFHGRRMCKARNPECASC
                     PIKEDCNYYKELNETK"
     gene            4896..5366
                     /gene="trmL"
                     /locus_tag="ACOIAF_003052"
     CDS             4896..5366
                     /gene="trmL"
                     /locus_tag="ACOIAF_003052"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HGZ2670053.1"
                     /translation="MSLNIVLVEPEIPQNTGNIIRTCAATGSTLHLVKPLGFSLDDKH
                     LKRCGLDYWDIADIQYYDSFEELQEKYPNSKYFFSTTKAKQSHSDVKYEENCFIVFGK
                     ETKGLPEPLLKANMDTCIRVPMLDVEKARSLNLSNSVAIVVYEALRQIGYPNMR"
     gene            5402..6250
                     /locus_tag="ACOIAF_003053"
     CDS             5402..6250
                     /locus_tag="ACOIAF_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HGZ2670054.1"
                     /translation="MKNLKIICDSLSDVPKDLLDKYDIDIVSLTVILEEREYKDRIDI
                     SGDEFYKRLREENVYPKTSQATYAQFKEIFEKYTNEDREILYIAGSATATGTYQSAVM
                     AKNDVDGKIYTYDSNLLCMGTGLLVIKAGELASEGKSVAEILPVLDELKEKAFVIFSV
                     DTLEYLQKGGRISSTKAAIGSILNIKPILEIRDGLVSQISQVRGKKNVISKMMELIKS
                     NCGDDLSDQIVYIGYSDDFKEKEQLSKVMQDELRPKEIRFFQIGVCIGAHAGPGVTGI
                     ICLKNK"
     gene            6657..7661
                     /locus_tag="ACOIAF_003054"
     CDS             6657..7661
                     /locus_tag="ACOIAF_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HGZ2670055.1"
                     /translation="MRFKRILILMLTVAMIAGMLVGCANNPSNNPSSSKDNKSDISDK
                     SNSKKSVSMILDIEGTNNEAMNNSALLALNNAQKKLNIDTNKVESDDSSTFSNSIDIL
                     CNDNYDLIIAVGARFAKPLEMVAKKYPKQQFAIIDYEYDKQPSNITSISYEDNKSGYL
                     AGLIAGKMTESDKVGFIGGTKSSSRDKFESGFREGVKFSNSSIKDISVEYADVFKDSK
                     SVESIAKKMMDNGVDIIFSTTEDDSKAVIDAVRAKNKKVIATNKDQHELAPENVISSI
                     VKDFENPTYNLIQSFVKGNYKGGKVIENTVKSGSTGLAQNSSQNIPPDVLEYVNKNNK
                     "
     gene            7787..8899
                     /locus_tag="ACOIAF_003055"
     CDS             7787..8899
                     /locus_tag="ACOIAF_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HGZ2670056.1"
                     /translation="MKIKRILMACFVVVFTIFILAICNFDFGFDKVNKNNSNIDSAES
                     KQNESKSLPTSAEKDKSNSKSKNKEDDTEDDSYVRKTIGVLLDTENLNNESYNNNVLS
                     ALKRADEDFDVRIKLEKPKTSKSFKESIEKLHKYEPDLIIAVGARFAEPIKEAATKYP
                     KQQFAIVDYTYKVQPANVISVSFQDNVTGYLAGLIAGKMTDTGKVGFIGGVEGSSRDK
                     YESGFRKGLKTSNKDAELLVKYSNIFENPESARTIAKEMRDSDRDVVFSATEDDSKRA
                     IQVARENGGKVIAINKDQYDMAPDNVISSIIKDVEQPVYELIKNVAKDGFKGSKVMEF
                     KIKDGELGLTSKSSRNIPPDVLEYIKKEYNKVKDTY"
     gene            9322..10083
                     /locus_tag="ACOIAF_003056"
     CDS             9322..10083
                     /locus_tag="ACOIAF_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6873 family GME fold protein"
                     /protein_id="HGZ2670057.1"
                     /translation="MEFTREPFILDDDLCLALVDKRISYLMENELKLRNIEIIKTIEC
                     KELYESIKYHPDICICNLGKGDIIVAPNVYNQYKELLSRYKFNIIKGEANLLNRYPYD
                     IPYNIAIVGDYAIHNFKYTDKAILDYIENNKLKKINIEQGYSKCSICIVDRTSIITSD
                     KGIWKSMKNTEIECLLIEKGHINLFEMNYGFIGGCTGLISKDKLAFCGDVKKHPDYER
                     IKSFVESKNKEIVTLSCENLLDIGSIVPLMTRKER"
     gene            10086..10904
                     /locus_tag="ACOIAF_003057"
     CDS             10086..10904
                     /locus_tag="ACOIAF_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sporulation protein YtxC"
                     /protein_id="HGZ2670058.1"
                     /translation="MVEKDNISSMKFLRNNNVNDRLIYTNDIVEVVIDSAKQEVLNKK
                     ILNGTKAEQRALNIDEISTEITSMIISIMKEKLLKEYILKCYGRTYPEDKNNIYIYSL
                     NIFAKKEIFMRETVFTRVRNYIEENDFINVEGFIRFRMREFMKYISAIGDIAVEEYLI
                     KKDQDEFIRVLKYFIDTQEEKIDLLKVHIMEDNTFVLYDKNGNKIDSIDDEEIINMVI
                     RENLNYEDFLISTLLTLCPKKIEILDLLNNNCSKEIIDTVEAIFENKVSIIMEN"
     gene            11084..11686
                     /locus_tag="ACOIAF_003058"
     CDS             11084..11686
                     /locus_tag="ACOIAF_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HGZ2670059.1"
                     /translation="MDNLIRILILAVIGGFIGYVTNVVAIRLIFRPIEPIKIPILNIE
                     IVGLIPKRRAEIAANVGEIIQEEFLSMDEILANIITDEDKEEVVRYIKARVKIIIHEK
                     VSFIPSGIKNMIQDYLGEIIESEVKQSIDELSKNIINKANERIDIQKMVEDKINELDL
                     YELEEIIIRIAKKELKHIEFLGLVLGFLIGIAQGLITMFI"
BASE COUNT         4944 a         1047 c         2182 g         3566 t
ORIGIN      
        1 aagtacctaa aagagttagt aggtaaaaaa attaaataga taaaaagacg aagtatctaa
       61 aagatttaag taggtaagaa aattgaatag ctaaaaagat taagtattta aaagagttag
      121 tagctagaaa aggttaaaaa gactaaaaaa attaaataaa tttaaaaagt caattagatt
      181 aaaaaggaat aattttatgg ataataagga attaaaagca ttttgtaatt taataggatt
      241 aaactgtgta ggagtagcag gggtagataa gtatgataat ttagaaaaga tacttaagga
      301 cagacaacaa aaaggttatt taactggtat ggaagaacca ataattgaga acagaattaa
      361 cccaagacat ataatgaagg atgctaactc tataatagtc tgtgcatttc catatcatat
      421 aaatagacaa gaaaagaata aaaattcaaa tttatcaaaa tattgtcatg gaaaggatta
      481 tcacattgta gtaaaggact ttttacaaca aatttgtgat tatatttcta aaacagtaac
      541 agattttaaa tacaagttat ttgctgataa tggaccatta gttgacaggt atttagctta
      601 tttatcagga ataggatatt ttggaataaa taataatatc ataactgatg aatatggttc
      661 ttatgtattt atagggtata tagtaaataa ttataaattt gaagaagata aaccactaga
      721 aaaaagttgt tttaaatgtg gtaagtgtgt aaaatattgt ccgggaaatg ctatattggg
      781 aaattatgat atgaacccca aaaaatgttt atcatatata actcaaaaaa aaggagattt
      841 gtcagagaaa gaaaaagttg ttttaaagaa tggcaaaaag gtatttggat gcgatatttg
      901 tcaagaagta tgtccacata atgttgaaat accaacaact catattttgg aatttaaaga
      961 aaatataata gattatttgg attatgatga gatacaaaat atttcaaata aagaatttaa
     1021 aaggagatat ggagatagag cttttagctg gagagggaga aatataataa agagaaatat
     1081 ggaaataatt ttagataagt atgatgacta ataataagag tctgtttcat atactttaat
     1141 gtgtatttac tttaacactt gttgtgtaaa agtcatatta tatcattaaa gttaaatatg
     1201 gggaggtggg ggaatattga aatataataa tacatctgaa ataaatccac tagattatat
     1261 attgggtcaa gaaagagctg tagaagctat ggaattaggg ttgaaaataa ataatccagc
     1321 gtacaacata tatatagctg gtgaacctgg aactggtaaa agtacttatg ccttaaaggt
     1381 tttaaatgag tatgcatcca aaaaaaatac gcataaagat tggtgttata tatataattt
     1441 tgaaaaccct agagagccta ttatagttga attagaaaag gggtttggaa gagaacttaa
     1501 gaaagatatg gagaaattaa ttgaaagtct tttagaagat tttaaaaatg cgtttgaaag
     1561 tgaaaatttt gaaatagaaa aaaataaact tctggatgaa tatgaaatag aaaaagatat
     1621 gcttttgaag caaattaaga aatatggaga agaaaaagga tttaaattaa aacagagtaa
     1681 aatgggaata gtttttatcc ctttgaaaga agaggaagat gaaagtgatg agtctgatga
     1741 agagttttac aaagctaaaa gagaactaga aaatatggcg atacaggtcg tatataaaat
     1801 tagaaactta gaagaaatag ctgaaaaagc agtgttagag cttgaagaag agatagctaa
     1861 acttgtagta gaacctcata tcaagagatt gtgtgaaaaa tatgaaaatt atgataagat
     1921 tcaaacttat ctggaaaata taaagaaaga tataatagaa tatatgtatt tattctattt
     1981 agatgaagag gagttaaaag ataagtatga taaagagcat tttattaaat ataaaattaa
     2041 cctatttgta gacaatggaa gctcaaagaa taaagaatca gcacctgttg tagtagagat
     2101 aaacccaagt ccagcaaatc tatttggaaa ggcagagtat gattatgcaa atggaaatat
     2161 aaagactgat ttcacaaaac tattatcagg agcatttcat agggctaatg gtggttattt
     2221 agtactttat gcagaccaat tactaaaata tacaatgtct tgggaaattc ttaaaaagac
     2281 tttgcagact aaaaaagtaa tattggaaac acaaacagca attaaaccag aaaatatgcc
     2341 attggatgtt aaattagtac tgattggaag ccactatata tatgatattt tatatagata
     2401 tgatgaagat tttgaaaaat attttaaagt atttgtagac tttgatagtg aaatggataa
     2461 aaatgaagat aatgaagaag gaatagcaag atttatagca taccaatgtg ataagaataa
     2521 tttaaggcat tttactaaat cagcagttga agaggttatt aaatttagca ctagattatc
     2581 tggtgattta gaaaaattat caactaagtt taataaaata atggaagttg taatagaagg
     2641 aagtgcttat gcagagttta gaaattcgga gtatacagag cagtgtgatg ttaaaaaagc
     2701 aataagtgaa aagagaaaaa gaatcaatag agtggaaaca catatggatg aatcaattga
     2761 aaatggattt actctaatag aaacagaggg aagaaaagta ggagtgataa atggtttgtc
     2821 agtcttaagt acaggagaat actcttttgg tagagcttca aggattacag cgaccactag
     2881 cccaggaagt aaaggcatag taaatataga aagagaagta aatatgagtg gctctataca
     2941 taacaaagga gtacttatat taggtggcta tctttctgag aattttgcac aggatttact
     3001 attgtcacta aatgcatatg tttgttttga acaaaattat ggtggaatag atggagatag
     3061 tgcatcttct gcagaattat atgtactatt atcatctctt agtggagtat caattaaaca
     3121 aaatattgct gttacaggtt ctataaatca aaagggtgat atacaagtag ttggtggtat
     3181 aagtgaaaag atagaaggat tttattctat atgtaaaact aaagggttaa atggtgagca
     3241 aggcgtcata attcctagaa aaaatcaaag aaatctcgtt ttatcagatg aagttaatga
     3301 tgcagtgcga gatggaaaat ttaagatata tactgttgaa agagtagaag aggctataga
     3361 gatattgaca gatgttaaat ttgaagaaat aaagaaattg gtaaaagaaa aattaatatc
     3421 tttcagcaag atacaaactg tgtcaaaaga ataagtttat tatagaattt aatgggtatt
     3481 tatgtataat aaaaagcaag aactttaata ggtttttgct ttttttgtaa atatatataa
     3541 attaaattct tattttgtat gttttatata ttgatgttta ttttttgttg atatgattaa
     3601 actatttata aataggtttt ataaattagt attttaaagg gggataatta tgaaaaatgt
     3661 aaatctaaaa gagataaatg aaaaagatgt aattgtggaa gaagatataa atctaaaagg
     3721 aatggataag aaagatgtaa ttgagaagga agaagtaaat aaaaaaagta tagataagaa
     3781 agatataatt gagaaagaag agatagataa aaaaaatgta aagaaggtcg aggttcaaaa
     3841 agaaatagat aaaatgattg gtaaaaaaga tgagataatt gatgagaaag atatagagaa
     3901 ttttatggat attgaagaag ataaaaaaag tatcaaagta agtaaaaaaa gtactaaaaa
     3961 agatgaaaag gagaaaaaag caaagaaaag tagttctagt aaggttacac aaaaacaaag
     4021 caaagaagtt aaaagtaagt ctaaaattaa aaatgagtca gcaaatgaaa aagacgtaaa
     4081 taagatttta gacgagttgg aaaaattgta tccagatgct aaatgtgagt taaattatgg
     4141 gacagccttt gaacttttaa tagcaactat tttatcagca caatgtactg atgtaagagt
     4201 gaataaagtt acttcagagt tgtttaaaaa gtacaacaca gctagagact ttgctaattt
     4261 gagcattgaa gaaattagta aagagataaa gagttgtggt ctttacaaaa gcaagtctca
     4321 aaaaataaag gatacatctg aacagctatg tgaattatat gatggtgaag tacctgatag
     4381 cttagaaaag cttataaaat tacctggtgt tggaagaaaa acagcagggg ttgtacttag
     4441 taacgctttt aatcatccag caatagctgt agatactcat gtatttagag ttagcaatag
     4501 aataggaata gtggacgagc caaatcctca aaaaacagaa tttgcactta tggaagctat
     4561 accaaaagaa agatggtcac actcacatca tgtattaatt tttcatggaa gaagaatgtg
     4621 taaagctaga aatccagaat gtgcaagttg cccaataaaa gaagattgta attattataa
     4681 agaacttaat gaaacgaaat aacttttaga aagtaagttg tacaagaggg attttctgat
     4741 ataataatac ttgtgaaaat gttagataaa aaactattat atacatgaaa taaaaattat
     4801 tattaacatt attttatata atagtataat attattgtta ttctgtttat aaaataaaat
     4861 ttgataaata aaagcgatac ttagggagga ttaaaatgtc attaaatata gtattagtag
     4921 aaccagaaat acctcaaaat acaggaaata taataagaac ctgtgcagca acaggttcaa
     4981 ctttacattt agtaaaacca ctgggttttt cattggatga taaacaccta aaaagatgtg
     5041 ggcttgacta ttgggatata gctgatatac aatattacga tagctttgaa gaattacaag
     5101 aaaagtatcc aaatagtaaa tacttttttt ctacaacaaa ggctaaacag tcacattcag
     5161 atgtgaaata tgaagaaaat tgttttatag tatttgggaa agaaacaaag ggattaccag
     5221 aacctttgtt gaaagcaaat atggatactt gtattagagt gcctatgttg gatgttgaaa
     5281 aagctaggtc attaaattta tcaaattcag ttgctattgt agtgtacgag gctttaagac
     5341 aaataggata cccaaatatg aggtagtttc aacaagccta aatgagaaag gagcatactt
     5401 aatgaaaaat ctaaagataa tctgtgacag tttatcggac gtgccaaaag atttgttaga
     5461 taaatatgat atagatatag tttcattaac agttatactg gaggaaagag aatacaagga
     5521 tagaattgat atatctggag atgaatttta taaaaggctt agagaagaaa atgtatatcc
     5581 aaaaacttct caagctactt atgcccaatt taaggaaatt tttgagaaat atacaaatga
     5641 ggatagagag atactttata tagcagggtc tgccacagct acaggtacgt atcaaagtgc
     5701 tgtaatggca aagaatgatg tagatggtaa aatatataca tatgactcta acttgctttg
     5761 tatgggaact ggtttacttg taataaaagc aggagaactt gcaagtgaag gaaagtctgt
     5821 tgctgaaata ttgcctgttt tggatgaatt aaaagagaag gcttttgtta tattttcagt
     5881 agatacattg gaatatttac aaaagggagg tagaatatca tctactaagg cagcaatagg
     5941 aagtatatta aatataaaac cgattcttga aataagagat ggattagttt cgcaaatatc
     6001 tcaagtaaga ggaaagaaga atgtaatttc taagatgatg gaattgataa aatcaaattg
     6061 tggtgatgat ttatcagacc aaattgtgta tataggatat agtgatgatt ttaaggaaaa
     6121 ggaacaatta tctaaggtta tgcaagatga attaagacct aaagaaatta gatttttcca
     6181 aattggtgtg tgtataggtg cacatgctgg tcctggtgtt actggtatca tatgcttaaa
     6241 aaataaataa tatatttttg gatgacatta tttatttttt tattaaatta ggatagaata
     6301 ttaattattg agatgttaaa ttggatttat tttggatgaa attaagtgtt gatatattag
     6361 atttagtttg tagggaagtg tgtaaataga taagaatcta ttgcacactt ttttatagta
     6421 agatttatta aaaaaatttc aaaatgtact tgtaaattgt atataaaaaa attgtaaata
     6481 ggatgtaaaa gtaaatagga tgtaaaagta aataggatgt aaaagtaaat aggatgtaaa
     6541 agtaaatagg atgtaaaagt aaataggatg taaaaataaa taggatgtaa aagtaagtag
     6601 gatgtaaaaa taaataggat ataaaaatat cgtaaatgta tgaggagtga aatttaatgc
     6661 gtttcaaaag aatattgata ttgatgttaa ctgtagcaat gatagctgga atgttggttg
     6721 ggtgtgctaa taacccaagc aataatccta gctctagcaa agataataaa agtgatataa
     6781 gcgataaaag taattcaaaa aaatctgtat caatgatact agatattgaa ggaacaaata
     6841 atgaagctat gaataattca gctttattag cattaaacaa tgctcaaaag aaacttaata
     6901 tagatacaaa caaagtggaa tcagatgatt cctcaacttt ttctaatagt atagatattt
     6961 tatgtaatga caattacgat ttaattatag ctgtaggagc tagatttgca aaaccattgg
     7021 agatggtagc caagaaatat ccaaagcaac aatttgcaat tatagactat gagtatgaca
     7081 agcaaccttc taatataact tcaatttctt atgaagataa taaatctgga tatctagctg
     7141 gattaattgc tggaaaaatg actgaaagcg ataaggttgg ttttatagga ggaacaaaga
     7201 gttctagtag agataaattt gaatcaggtt ttagagaggg tgttaaattt tctaatagta
     7261 gtataaagga tatttcagtt gagtatgcag atgtttttaa agatagcaaa tcagtagaat
     7321 ctatagctaa aaaaatgatg gataatggag tggatattat tttttctact actgaagatg
     7381 attcaaaagc tgtaatagat gcagtaaggg caaaaaataa aaaagttata gcaacgaata
     7441 aagaccaaca tgagttagct ccagaaaatg ttataagttc tattgtaaaa gattttgaga
     7501 atcctactta taatctgata caaagttttg taaaaggaaa ttataaaggc ggaaaagtta
     7561 tagaaaacac agttaagtct ggaagtactg ggcttgccca aaatagttct caaaatattc
     7621 ctccagatgt gttagaatat gttaataaga acaataaata atctactata atatccaaga
     7681 atacttatga ctttagttat gaggtgaatt ggatgttata atagacatat atagaaagac
     7741 atatgcagag gtaggtcaga cctatctaaa tcagggagtg aattttgtga aaataaaaag
     7801 gatattgatg gcttgctttg tagtagtttt tacaattttt atattagcta tatgtaattt
     7861 tgattttgga tttgataaag taaataagaa taattctaat atagatagtg ctgaatcgaa
     7921 acagaatgag tctaaaagtt tgccaacttc agcagaaaag gataaatcaa atagtaaaag
     7981 taagaataaa gaagatgaca cagaagatga ttcatatgta agaaaaacta ttggagtatt
     8041 gttagatacc gaaaacttaa ataacgaatc atataataac aatgtactat cagccttaaa
     8101 aagggctgac gaagattttg atgttaggat aaaacttgaa aaaccaaaga cttctaaaag
     8161 ttttaaagag agtatagaaa aactgcataa gtatgaacca gatttgataa tagcagttgg
     8221 agccagattt gcagagccaa ttaaagaagc tgctactaaa tatcccaaac agcaatttgc
     8281 aatagttgac tatacttata aagttcagcc agctaatgta atttctgtat catttcaaga
     8341 taatgttact ggatatttag caggacttat tgcaggtaaa atgactgata ctgggaaagt
     8401 cggttttata ggaggagttg agggttctag tagagataag tatgaatctg gatttagaaa
     8461 ggggcttaaa acttcaaata aagatgctga gttactagtt aagtattcaa atatatttga
     8521 aaaccctgaa tcagctagaa ctattgcaaa ggaaatgaga gattctgata gagatgttgt
     8581 attttcagct acagaagatg atagtaaaag agctattcag gtagcaagag aaaatggtgg
     8641 taaagttata gctattaata aagaccaata cgatatggct ccagataatg taataagttc
     8701 aattataaaa gatgtagaac aacctgtata tgaactgata aagaacgttg caaaagatgg
     8761 ttttaaggga agtaaagtaa tggaatttaa aataaaagat ggggaacttg gcttgacttc
     8821 aaagagcagt agaaatatac caccagatgt tttggaatac ataaaaaaag agtataacaa
     8881 agtgaaagat acatattaat tattgatgta gaagattata aaaatatatt gcttaagttt
     8941 agattagcaa taaaaagata tatttattaa atttgtgcta actgtacaaa atatattgat
     9001 taagtttaga ttggtgataa aaggatatat ttattaaatt tagtctaact gtacaaaata
     9061 tattgattga gtttagatta gcaataaaaa aatatattta ttaaatttgt gctaactgta
     9121 cggaatatac tgcttaagtt tagattagtg ataaaaagat atatttatta aatttgaacc
     9181 aactgtacaa agctatacta attaagctta gattaactat ataaaattat aacaataagt
     9241 gtgtgggcag aaaaattctg ctacacactt tatttatatt cacataaaaa atttatcaaa
     9301 aaaaagaaaa aggagtaaaa aatggaattt acaagagaac catttatact agatgacgat
     9361 ttgtgtctag ctttggtgga caaaagaatt agttatctaa tggaaaatga attaaaatta
     9421 agaaatatag agataataaa aacaatagaa tgtaaagaat tatatgaaag cataaaatat
     9481 cacccggata tatgtatttg taatttagga aaaggagata taattgttgc gccgaatgta
     9541 tataatcaat acaaagagtt attaagtaga tataaattta atataattaa gggagaagct
     9601 aatttattaa atagataccc ttatgatata ccttacaata ttgcgatagt tggtgactat
     9661 gctatacaca attttaagta tactgacaaa gcaatattag attatattga aaataataaa
     9721 cttaaaaaga taaatataga gcagggctat tctaaatgct caatctgtat agtagataga
     9781 acgtctataa taacatcaga taaaggtatt tggaaaagta tgaagaatac agaaatagag
     9841 tgtttattga tagaaaaagg tcacataaat ttatttgaga tgaattatgg atttataggt
     9901 ggatgtacag ggttgatttc taaggataaa ctagcttttt gtggagatgt taaaaaacat
     9961 cctgactatg aacgtataaa aagttttgta gaaagtaaaa acaaggagat agttacactt
    10021 agctgtgaaa atttactaga tataggttct atagttcctc tcatgacaag aaaggagcga
    10081 taactttggt tgagaaggat aatatttctt ccatgaaatt tttgaggaat aataatgtaa
    10141 acgatagatt aatatatacg aatgacattg tagaagtagt gatagattct gcaaagcaag
    10201 aagtgttaaa taaaaaaata cttaatggta ctaaagctga acaaagagct ttaaatattg
    10261 atgagatatc aacggaaata actagtatga taataagtat aatgaaagag aaacttttga
    10321 aagaatacat tctaaaatgc tatgggcgta catatcctga agataaaaat aatatataca
    10381 tatattcact gaacatattt gctaaaaaag agatttttat gagagaaaca gtatttacta
    10441 gggtgagaaa ttatatagaa gaaaatgatt ttataaatgt agaaggtttt attagattta
    10501 gaatgagaga atttatgaaa tatatttctg ctataggtga tattgcagtt gaggagtatt
    10561 taataaagaa agaccaagat gaatttataa gagttttaaa atactttata gatactcaag
    10621 aagaaaagat tgatttgctt aaagttcata taatggaaga taatacattt gttttatacg
    10681 ataaaaatgg aaataagata gacagtatag atgatgaaga aattataaat atggtaataa
    10741 gagaaaattt aaattatgag gactttttaa taagtacctt attaactcta tgtcccaaaa
    10801 aaatagaaat attggatttg ttaaataata attgctcaaa ggaaattata gatactgtag
    10861 aagcaatttt tgaaaataaa gttagtataa ttatggaaaa ctagaatcta agcatataaa
    10921 agctatccat ataaaattaa aaaaattctt tttaaatcga cacaaaagac ttataattgt
    10981 atataaagct atagtataac tagtgttatt aagtataaag aatatctact tgattaatac
    11041 aatatagctg gcaaatatac aattgataag gagtaatttt ataatggata atttaattag
    11101 gatactgata ttagctgtta ttggtggatt tataggttat gttacgaatg tagttgctat
    11161 aagactgata tttagaccaa tagagcctat taaaatacct attttaaata tagaaatagt
    11221 aggtcttata ccaaagagaa gagcagaaat agcagcaaat gtaggagaaa taattcaaga
    11281 agaatttctt tctatggacg aaatacttgc aaatatcatt actgacgaag ataaggaaga
    11341 agttgtaaga tacataaaag caagggtaaa aattataata catgagaaag tttcatttat
    11401 cccgagtggt ataaaaaaca tgatacaaga ttatctagga gagataattg agtctgaagt
    11461 taagcaaagt atagatgaat tgagtaaaaa tataataaat aaagcaaatg aacgtataga
    11521 tatacaaaaa atggtcgaag ataagattaa tgaactagat ttatatgaat tggaagagat
    11581 aataataaga atagctaaga aagaattaaa acatatagag tttttggggc ttgtcttagg
    11641 gtttttaata ggtatagctc aagggttaat tactatgttt atttaaatat atgagaagta
    11701 gcatttattt aagtactaat tatatttacc tatttgagt
//
LOCUS       DBGJFF010000120        11488 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.122, whole genome shotgun sequence.
ACCESSION   DBGJFF010000120 DBGJFF010000000
VERSION     DBGJFF010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11488
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.122"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            791..1501
                     /locus_tag="ACOIAF_003059"
     CDS             791..1501
                     /locus_tag="ACOIAF_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HGZ2670060.1"
                     /translation="MYRIVICEDDITQITFLRECILKSLEGISSQIELFEFNSGEELL
                     ESNLESIDIFFLDIKMLKITGMDVAKIIRETNDTSEIIFITSIVDYIQEGYKVRAYRY
                     LLKPIDFGDLNESILSCISDIIKRRENFMLIENKGIINKILINSIMYIEVRKKVLTIH
                     TKNDTYYTKNSMDKIELELEKYNFFRCHKSYLINLQYIQFICKNAVVINNEDIPVSKY
                     RISDLKTKLTHVLGDVLC"
     gene            1495..2814
                     /locus_tag="ACOIAF_003060"
     CDS             1495..2814
                     /locus_tag="ACOIAF_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HGZ2670061.1"
                     /translation="MLETYVFWIILTIVSATIEWTVLKFILDEISELRNKKLVLNSSL
                     LLAIMTITILTLVEFNINIKLLICIFITYILYKMNYIVDKWKSMFISLLYWMILIGFD
                     IIGLSIVGTLNSIQNMTTLLDNNLLKLELIMISKSLLIALIPLLKVIRLKIKINKKDC
                     IYSSIPIISNIIGVIVIFEFIFKDKNIDSTENLIILIISIVFLLSNISLISIIGRIIK
                     DNNLRIEHEITKEKMNMQYKYYLNLQEYQLKIRKLYHDMSNHVICIQNIYGKNEFADK
                     YIKDINKQIKESNAMFNTQSMILDVILSEKKSICDMNNIDFLVDINFLECNFIEMPDV
                     CSIFSNIIDNAIEACNKIKDTSILKKIKLRGTVINRLFVIKCENTKINEVVLKNNKLI
                     TSKKDSFLHGIGISSVKGSVEKYNGNVEVYSDKNKFTITIYIPLARN"
     gene            3355..5904
                     /locus_tag="ACOIAF_003061"
     CDS             3355..5904
                     /locus_tag="ACOIAF_003061"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2670062.1"
                     /translation="MINIKLAMSYLKKQKGKTLSLILSIGIAVILVFSLSVIKESQGQ
                     SDINHAYKNSGDYHAFYEDVDSKIVKKLNSEEDIKEFNNVLKFGEIVSKDNGSSINLC
                     SYNKNFLDSTRYKLIEGREPRNSNELVIEQKVLDKMKLDVKLNQTIDFQIVNEYVNKN
                     NENSIYSKDKQFKIVGILSKTDEYYEGLNKYKVRAFTYSKDGKDIVPYELITHMGTLK
                     LNTKDLDTQKVVNLGRKYKLTSNQFYPNTEVSMSMYMKNISKKTTYSIKQSLLPLLCA
                     TLVIYNMFNIIILDMTKQIGLLRAVGASKRNIRQIFFIQSIVVLSVGIVIGLLGGIVF
                     SYLGLMIVYDKSAELIINTTAIIESIIMSIIAVILASVVPVYKAGNLSIIDAIKKTDK
                     LNYIPKFLLLKSHKKVIGIINEIAFKNIFRNKVTSIIIIITISLCGVLFIGRLTSTKF
                     IYGKESDSANITSKSYGNFDIYLGYSPINANYTFSKYDNSLLNEIKAIDDVTDIESNI
                     YLKGYLRNADLEKGYLDELKRTEINNTNEPTVLIRGYNKELLNNIDKYIDKGENVYSY
                     TKGDYKNILLVNNFYSRIELSNNTQIIKSPKLGDLIDIKTPVYKDGEYKYINTKVRIA
                     GIMKNSYISEQDGESQSGGIQVIFNEKDLKELTGMNYYNKAFVKIKKGTDKKVIKEIN
                     NIIEKSGFSDIEGRYVRNHFGDHYNKSSYKLAMICVFGVLLISSINIIFIVRSNIIVR
                     LSEICTFRAVGMSKKNVKRMLIKENIIYGVLSMLVAGIISSFSYYKMVSETNKLNKQI
                     YGISNSIKFEIPIYEILIFGLISVFVCIIAVYFSNKMINKLSIVSGIKENE"
     gene            6139..6813
                     /locus_tag="ACOIAF_003062"
     CDS             6139..6813
                     /locus_tag="ACOIAF_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2670063.1"
                     /translation="MKILKVKDLKKIYGKYENEVRALDGIDLEIEPHKFTAIVGASGS
                     GKSTLLHCVAGLDKPTLGNVYLDDVDLYTLNDNKLSEIRRQEFGFIFQSYNLIPVINV
                     YDNIVLPVLLDGKKEDKEYVMGLINKLGIENQIKKYPNELSGGQQQRVAIARALANRP
                     SVIFADEPTGNLDSKTTNEVMNMLEWCVKEYKQTLVMITHNDNIAKKADRIITIKDGK
                     IDEKNY"
     gene            complement(7082..8326)
                     /locus_tag="ACOIAF_003063"
     CDS             complement(7082..8326)
                     /locus_tag="ACOIAF_003063"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HGZ2670064.1"
                     /translation="MEDEILKGKIKQLTILALIFIFITPVFAFADTPPVPNSSRAALL
                     IDQETKRILFEKNIDEKMPLASLSKMMTFLLAIEAVDKNQVKETDMVKIDKSTASVGG
                     STCKLKDGDEISLGELMQGLMLVSGNDAAIAIAKHIGKTEKNFVNMMNKKAEEIGMID
                     TYYFNPNGLPIYTDPEHKEPPIENMSTAHDIVTLGKYMYDHYENQVTRITTMQVYNDT
                     KKDFTHYNTNPLLVSVPGVDGIKTGYTDNAGYCLAFSMMVPKDAKNERNHRLIGVVLG
                     DGNKKNRISSSATLLKYGKDNFHSKKIAHKGDIIETPCVDGIDDFKITVKVDKDLYGV
                     VSDNENINPKVVFKNMNYPIHKGDIVGVAKYYNDSGKFVGSVDVKSESNIGCIPLKDK
                     IKIKVAKINKKLDIKNSVCFKA"
     gene            complement(8369..11203)
                     /locus_tag="ACOIAF_003064"
     CDS             complement(8369..11203)
                     /locus_tag="ACOIAF_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HGZ2670065.1"
                     /translation="MLRKDKVLLSLHKLCENMTLKDLQSDKTGFTTIEIANHSSLDRS
                     NTSKELNNLYNEKKVLKISGKPILFLHASIIENLIGRTLNTDEFSINNCNELLKSSKN
                     DSEDIFSTLLGHDSSLKLPIEKAKSAILYPPKGLNTLLIGPTGVGKSTFAETMYKYAV
                     ESKIFSSESKFVVFNCAEYAENANLLLSNLFGYVKGSFTGANKDKFGIVAQADGGILF
                     LDEIHRLPPEGQEMLFLLMDKGIYRRLGESDIVHKANVFIVCATTEDIHSSLLGTFLR
                     RIPVTINLPALQDRSLKERLSLIKYFFIIESKYTNATIKVHKDVIKSLLLYDCFGNIG
                     QLKSDIQLMSARAFLNYKTGLKDNIEIDLSLVPDYIYIGLLKLNENRSNLNKLSELDY
                     MLFYEFNGNNLEVEYTKELSKYNIDTSNNGFKYSTEIPYLENDIRSMFINYMEKYSTK
                     LLSISSVTHSDYTSNEIFKVINPKIYYAVEHSLKEAEKISRNKYTKQTYIALSMHISA
                     LIENINQDTYNPKYNLYNDKIFLDKDLNIAKIVLSIIENDLQTSLPKDEVKFISMFLN
                     SMYLEPNNQSESIGVIILAHGNSTASSMCDVANSLLGTNHCNAIDMPLDVKVSTILDK
                     TIELVKECDEGKGVLLLTDMGSLVAFGELISEKTKIKTRSIEMVSTPIVLEAVRKSTL
                     PEMTLDELANSLSSLNPYIGRAAIDSTEINYPEKSREYVIITTCITGYGSAMKVADFI
                     NSSLKNISKYNINLVPHNIESFKMYKNKFSNTKILAVVGSVDFNVANTPFIHIEELLN
                     GYGLDMLNKIIEGTFNANSITNIKETNLSYNSLIINFMTQNLELLDSVKLFNYVNSSL
                     FTIIELIDENLKSKFKLGYILHCSFMIERCFKNTTFPYNNITELISKNHELYSLIREA
                     FKPTEAYFNILIPDEEIAYIIDMINEYLNK"
BASE COUNT         4377 a         1264 c         1652 g         4195 t
ORIGIN      
        1 acttaagtgc tatatcaaca attggtagac taatgcttaa gtttactaat ctggttaaat
       61 aatttaatac ctaattaaac taaattatat acttgtatag acatgttttt tgcttattaa
      121 atattactgg agatttttaa tcccagtaat attttttatt aaaaaatata taagcaataa
      181 tttgtataca tgttattaca aatttagcat gattaatata tactaaaaga tagatttatg
      241 ttaaaattca agtaaataga tcgaaaacaa aatataaaag gtcagccgtt cattttcgat
      301 ttattactag acttttctat agaaaaatct tagatagaga gctttagtta aaaaattttt
      361 tatatcatta attacgtatt aatatcaata taatagtcat taaacatcaa taattttaat
      421 atttaaaata atataaaaaa ttacctataa atagtataca aaactatatt aagtatcaat
      481 cgtttttaaa aatctaagct atcttcttat ttttgctgtt aacatactaa aaatagatgt
      541 ctatttttta tgtgaataac agcattttgt gactatttgt acaatgaaag tacagatgtt
      601 tatacacaaa tactaaagat tatgagctaa tttaaagtag gtagctaaat cattaagttt
      661 agggatagag atagtaaata aatattggag tttatatgtg gtaatccatt tgttagttgt
      721 ataactaaat agttatttat agatgtgata ttttatttgt tttatatcta atgtctaagg
      781 agggtcgttt atgtatagaa tagttatctg tgaggatgat attacacaaa taactttttt
      841 aagagagtgt atattaaagt ccttagaggg tatttctagt caaattgaac tatttgagtt
      901 taattcagga gaagagctat tggagtctaa tttggaaagt attgatatat tctttttaga
      961 tattaaaatg cttaaaataa caggtatgga tgtggctaaa ataattagag aaactaatga
     1021 tacttctgaa ataatattta taacttcaat agtcgattat atacaggaag gctataaggt
     1081 aagggcttat agatatttat taaagcctat agattttgga gatttaaatg aaagtatttt
     1141 gagctgtata tcggatatta taaaaagaag agaaaatttt atgttgatag aaaataaagg
     1201 tataatcaat aaaattttaa ttaatagtat tatgtatata gaggttagga aaaaggtttt
     1261 gactatacat actaaaaatg atacttatta tactaaaaat agtatggata aaatagaatt
     1321 agagctagaa aaatataatt tttttagatg tcataagagt tatttaatta atttgcagta
     1381 tattcaattt atatgtaaaa atgcagtagt tattaataat gaagatattc cagttagtaa
     1441 atatagaata tctgatttaa agacaaaatt aacacatgta ttgggcgatg tattatgtta
     1501 gagacatatg tattttggat tattctaaca atagtttccg caacgataga atggactgta
     1561 ttaaaattta tattagatga aattagtgaa ttaagaaata aaaagttagt cttaaacagt
     1621 agtctgttac ttgcaattat gacaattact attttgactt tagttgaatt taatataaat
     1681 attaaattac ttatctgtat atttattaca tatatattgt ataaaatgaa ttatatagta
     1741 gataaatgga aaagtatgtt tataagtttg ttatattgga tgatattaat tggatttgat
     1801 attattggat tgagtatagt aggaacactt aactctatac aaaatatgac tactttatta
     1861 gataataatt tgctgaaatt agaattaata atgatatcta aatcattact aatagcatta
     1921 ataccattac ttaaagttat cagattaaaa ataaaaataa acaagaagga ttgcatatat
     1981 tcatctattc caataatatc aaatattatt ggtgtgatag ttatatttga atttattttt
     2041 aaagacaaga atattgattc cacagaaaat ttaataatac tcataatatc aattgtattt
     2101 ttactatcaa atatttcatt aataagtatt ataggaagaa taataaaaga taataattta
     2161 agaatagaac atgagattac aaaagaaaaa atgaatatgc agtataaata ttatttaaat
     2221 ttacaggaat atcagttgaa aataagaaaa ctatatcatg atatgagtaa tcatgttatt
     2281 tgtattcaga atatttatgg taaaaatgag tttgcagata aatatattaa agatataaat
     2341 aagcaaataa aagaatctaa tgctatgttt aatacacaga gtatgatttt agatgttatt
     2401 ttaagtgaaa aaaaatctat ttgtgatatg aataatatag atttcttagt agatataaat
     2461 tttttagaat gtaattttat agagatgcca gatgtatgta gtatattttc aaatataata
     2521 gataatgcga tagaagcttg taataagatt aaggatacaa gtatactaaa aaagattaaa
     2581 ctaagaggaa ctgttataaa tagattattt gtaataaaat gtgaaaatac aaagataaat
     2641 gaggttgtat taaaaaataa taaacttatt acaagtaaga aagattcatt tttacatgga
     2701 ataggaataa gtagtgtgaa aggttcagta gaaaaatata atggaaatgt ggaagtatat
     2761 tcagataaaa ataagtttac aattactatt tatataccat tagctaggaa ctaattctag
     2821 ttggagccct aaagtgccca gttggagcaa tggtattgaa aaatgaaata gaaaatgata
     2881 tcttttaagc atagaagatt tagagatgat agtactcaat aaaataatag aacaaaaaat
     2941 gtgatttata tttatatgga tttaaaatgt atgtttagta gaaatttatg atgagtgata
     3001 taagaatagc aattaagtag ttgataaata gtgagattaa tatagtaatc aatcctaaaa
     3061 aaacagaaaa tttataattt ataaaataat tttataaata gattgtatgt aggaatatgc
     3121 aggagaaatt taaaaagaaa cagtagcata ttaaaaaaga ctagtcaatc tatttaaata
     3181 gtaaaagtac tagatatgat tttaggaatg aagtattctt ttttaggctc tagtattttt
     3241 aataattaaa tttaactagc acaataaatt agttagtttt taatgagact ctataatcta
     3301 ctattttaga tcatagagtt tttcttgtaa atataaataa ggagaatttt aattatgatt
     3361 aatattaaac tggcaatgtc gtatttaaaa aaacaaaaag gaaaaacttt atcattgata
     3421 cttagtatag ggattgctgt tatactagtt ttttcattaa gcgttattaa agaatctcaa
     3481 ggtcaatcag atataaatca tgcatacaaa aactcaggag attatcatgc tttttacgaa
     3541 gatgtagata gtaagatagt aaaaaaactt aatagtgaag aagatataaa agaatttaat
     3601 aatgtgctta aatttggaga aattgtaagt aaagataatg ggtcatctat aaatttgtgc
     3661 tcttacaata aaaatttttt agattctact agatataaat taattgaagg tagagagcct
     3721 agaaattcta atgaattagt aatagagcaa aaagttttag ataaaatgaa actagatgtt
     3781 aaattaaatc aaactattga ttttcaaatt gttaatgagt atgtaaataa aaataatgaa
     3841 aattctattt atagtaaaga taagcaattt aagatagttg gtattttaag taaaaccgat
     3901 gaatactatg aaggtttaaa taaatataag gtaagagctt ttacatattc aaaagatggg
     3961 aaagatattg ttccatatga attaataact catatgggta cattaaaact taatacaaaa
     4021 gatttagata ctcaaaaagt agtgaatcta gggagaaaat ataagttaac aagtaatcaa
     4081 ttttatccaa atacagaagt aagtatgtct atgtatatga aaaatatttc aaaaaaaaca
     4141 acttattcaa taaagcaaag cttactacct ttattatgtg caacattagt aatatacaat
     4201 atgtttaaca taataatact agatatgact aagcaaattg gtttacttag agctgttgga
     4261 gctagcaaga gaaatattag acagatattt tttattcaga gtattgtagt tttatccgtg
     4321 ggtatagtaa ttggtttact tggaggtata gttttttctt acttaggtct tatgatagta
     4381 tacgataaaa gtgcagaatt aatcataaat acaactgcta taattgaatc tatcatcatg
     4441 tcaattatag cagttatatt agcctctgta gtacctgtat ataaagctgg aaatttatct
     4501 ataattgatg ctataaaaaa gacagataag ttaaattata ttcctaagtt tttattgtta
     4561 aaatcacata aaaaagttat tggaattata aatgagatag cttttaaaaa tatatttagg
     4621 aataaggtta catcaataat aattattatt acgatatcac tatgtggagt tttatttata
     4681 ggcagactaa cttctacaaa attcatatat ggaaaagagt cagactcagc taatataaca
     4741 agtaaatcat atggcaattt tgatatttat ttgggatatt caccaataaa tgcaaattat
     4801 acattttcta agtacgataa ttctttatta aatgaaataa aagctattga cgatgtaaca
     4861 gatattgaat ctaatattta tttaaaagga tatctaagaa atgctgactt agaaaaaggt
     4921 tatttggatg agctaaaaag aactgaaata aataatacta atgagcctac agttttaatt
     4981 cggggttaca ataaagagct attaaataat atagataagt atatagataa aggagaaaat
     5041 gtctatagtt atacaaaagg tgattataaa aatattttat tggtaaataa tttttattct
     5101 agaattgaat tgtcaaataa tactcaaatt ataaaatcac ctaaactagg agacttaata
     5161 gatataaaaa ctcctgtata taaagatgga gagtataaat atataaatac aaaagtaaga
     5221 atagctggaa taatgaaaaa tagctatata tcagagcaag atggagaatc tcaatcaggc
     5281 ggaattcaag tgatatttaa tgaaaaggat ttaaaagaac taacagggat gaattattat
     5341 aataaagctt ttgtgaagat aaaaaaggga acagataaaa aagtaataaa agaaataaat
     5401 aatataatag aaaaaagtgg gttttcagat atagagggaa gatatgtacg taatcatttt
     5461 ggagaccatt ataataagtc ctcatataag ttagcaatga tttgtgtatt tggcgtttta
     5521 ttaatatcgt ctataaatat tatttttata gttagatcaa atataattgt gagattgagt
     5581 gagatatgta cattcagagc agtagggatg tctaagaaaa atgtaaaaag aatgttaata
     5641 aaagaaaata tcatatatgg agtattaagc atgcttgtgg ctggaatcat ctctagtttc
     5701 agttattata aaatggtttc tgaaactaac aaacttaaca aacagatata tggtataagt
     5761 aattctatta aatttgaaat tccaatatat gaaattttaa tatttggatt aatatctgtt
     5821 ttcgtttgta tcattgcagt ttatttttct aataaaatga taaataaact tagtatagta
     5881 agtggcataa aagaaaatga atagaattgc taatttaaat ataccagaag ggaatattct
     5941 ttacttaata actttacata tattgggaat tatgatacat ataatttttt tactatttta
     6001 aatagagcat ttaatcttgg tattgcctac aaagatgtat agttatatca attaaagaat
     6061 ttagatatgg tagtagaact tatttacgaa aatttaatct aagaatatat attttttaaa
     6121 tgggaatgga gatacgttat gaaaatactt aaggtaaaag atttaaaaaa aatatatgga
     6181 aaatatgaga atgaagttag ggctcttgat ggtattgatt tagagataga gccacataaa
     6241 tttacggcta ttgtaggcgc aagtggttct ggtaaaagta cactacttca ctgtgttgca
     6301 ggcttagaca agccaacctt agggaatgtg tacttagatg atgtggattt atatacatta
     6361 aatgataata agctgtctga aataagaaga caggaatttg gtttcatatt tcaaagctac
     6421 aatttaatac cagttataaa tgtatatgat aatatagtac ttcctgtatt gcttgatggg
     6481 aagaaagaag acaaagaata tgttatggga ttaattaata agttggggat tgaaaatcaa
     6541 ataaagaaat atcctaatga actatcagga ggtcaacaac aaagagttgc aatagctaga
     6601 gctttagcca atagaccgtc agtaatattt gcagatgaac ctactggtaa cttagatagt
     6661 aagactacaa atgaagttat gaatatgctt gaatggtgtg ttaaagagta caaacaaaca
     6721 ttagttatga taactcataa tgataatatt gcaaagaaag ctgatagaat tataactata
     6781 aaagatggaa aaatagatga aaaaaactat taatatttga agaataccta gtaaggaaat
     6841 caaaatttca cataattttc atattaatta tcaattaaat tcacacacaa gagttattat
     6901 ataaacatag aagatagtaa agctagctta aaaacataat ataataccct atacaaaata
     6961 gtggcaactc tccctcacat cctcaattgt tgccactatt ttaatttttt gtaaaaaaca
     7021 aaaaaactcc acaaagtgga gtctttataa atttaacata cccttttaat aatatgcttt
     7081 actatgcttt aaaacaaaca gaatttttaa tatcaagttt tttatttatc ttagcaacct
     7141 taatcttaat cttatccttc aaaggaatac atcctatatt actttcactt tttacatcaa
     7201 cacttccaac aaatttacca gagtcattat aatactttgc aacaccaaca atatctccct
     7261 tatggatagg ataattcata tttttaaaca ctaccttagg attgatattt tcgttatctg
     7321 aaacaacacc atacaaatct ttatcaacct taactgttat cttaaaatca tcaataccat
     7381 caacacaagg agtctctata atatcaccct tatgtgctat ctttttagaa tggaaattat
     7441 ctttaccata tttcaataaa gtagcagaag aagaaattct atttttcttg ttaccatcac
     7501 caagtacaac acctatcaat ctatgatttc tttcgttttt agcatcttta ggtaccatca
     7561 tagaaaatgc aaggcaatat ccagcattat ctgtatatcc tgtctttatt ccatcaacac
     7621 caggtactga aacaagcaga ggatttgtgt tataatgtgt aaaatccttt ttagtatcat
     7681 tatatacttg catagtggtt attcttgtaa cttgattttc ataatggtcg tacatatact
     7741 ttccaagtgt aactatatcg tgagcagttg acatattttc aataggaggt tctttatgtt
     7801 ctgggtcagt atatatagga agcccatttg gattaaaata atacgtatca atcattccta
     7861 tttcttctgc tttcttgttc atcatattta caaaattctt ttcagtttta ccaatatgtt
     7921 tagctatggc tatagcagca tcatttccag atactagcat tagaccctgc ataagctctc
     7981 ctagagatat ttcatcacca tctttaagtt tacaagtaga acctcctact gaagctgtag
     8041 acttgtctat tttcaccata tcagtctctt taacttgatt tttatcaact gcttctattg
     8101 ccaataaaaa ggtcatcatt ttacttaaac tcgctaatgg cattttttca tcaatgtttt
     8161 tttcaaataa tattctttta gtttcctggt ctataagtag tgctgctctt gaagaatttg
     8221 gtactggtgg agtatctgca aatgcaaata caggagttat aaatataaaa attaatgcaa
     8281 gtattgttaa ctgttttatt tttcctttca aaatctcgtc ctccatttca tatattaatt
     8341 aatatgcaca aaataagtgc tataaatttt atttatttag atactcatta atcatgtcta
     8401 ttatataagc aatttcttcg tctggtatca aaatattaaa ataagcttct gttggcttaa
     8461 aggcttcacg tattaaacta tatagttcat gatttttact aattaattca gtaatgttat
     8521 tatatggaaa agttgtattt ttaaaacatc tttctatcat aaaagagcaa tgtagtatat
     8581 atcctagctt aaatttgctc tttaagtttt catctattaa ttctattatt gtaaataagg
     8641 aagaattgac atagttgaat aatttaacag agtccaaaag ctctaagttt tgggtcataa
     8701 agtttattat taaactgttg tatgatagat ttgtttcttt tatatttgta atactattgg
     8761 cattgaatgt accttctatt attttgttta gcatatctag gccatatcca tttagtagtt
     8821 cttctatgtg tataaaaggg gtgttggcta cattaaaatc tacacttcca acaactgcca
     8881 gaattttagt attactaaat ttattcttat acattttaaa actttctata ttatgaggaa
     8941 ctaaattaat attgtattta ctaatatttt taagtgaaga atttataaag tctgctactt
     9001 tcatagctga accatatcct gttatacaag tagttattat tacatactct cttgactttt
     9061 caggatagtt tatttctgtt gaatcgattg ctgctcgtcc aatatatgga tttaaagagc
     9121 ttaaggaatt agctaattca tccaaagtca tttctggtag tgtacttttt cttactgctt
     9181 ctaaaactat tggagtagat accatttcaa ttgagcgagt tttaattttt gttttttctg
     9241 aaataagttc tccaaaagct acaagagagc ccatatctgt taaaagaagt acacccttac
     9301 cttcgtcaca ttcttttacc aactctatag ttttatcaag tatggtactt acttttacat
     9361 caagaggcat gtcaattgca ttacaatggt ttgtaccaag caatgaattt gctacatcac
     9421 acatactgct tgctgttgaa tttccatgag caagtattat gactcctata ctttcagatt
     9481 gattgtttgg ttcaagatac atgctattta gaaacatgga tataaatttt acttcatctt
     9541 taggtaaaga tgtttgtaag tcattttcaa ttatacttaa aactatttta gctatattta
     9601 aatctttatc taagaaaatt ttatcattat ataaattgta cttaggatta tatgtgtctt
     9661 gatttatatt ttctataagt gcacttatat gcattgaaag agcaatataa gtttgttttg
     9721 tgtatttgtt tctcgatatt ttttcagctt cttttaaaga atgttcaaca gcatagtaaa
     9781 tctttggatt gattacttta aaaatctcat tacttgtata atcagaatga gttactgaag
     9841 atatcgaaag taattttgta gagtactttt ccatgtaatt tataaacata ctccttatat
     9901 cattttccag ataaggaatc tctgtagagt atttaaatcc attgtttgat gtatctatat
     9961 tgtatttaga tagttctttt gtatattcaa cttctaagtt attaccatta aattcataaa
    10021 aaagcatata atcaagttct gataatttat ttagattact acgattttca ttcaatttta
    10081 aaagaccaat atagatgtaa tcaggaacaa gggataaatc tatttctata ttatccttaa
    10141 gtccagtttt gtaatttaaa aaggctcttg cactcataag ttgtatgtca gatttaagct
    10201 gacctatatt accaaaacaa tcataaagga gtaatgattt aattacatct ttgtgaactt
    10261 ttatagttgc attggtgtat tttgattcta taataaagaa gtatttaata agagataatc
    10321 tttcttttaa agacctatcc tgtaaagcag gtaggttgat tgtaacaggg attcttctta
    10381 aaaaagtccc aagcagagaa gaatgtatat cttctgttgt ggcacacaca ataaagacat
    10441 tagctttgtg cacaatgtca gattctccta atcgtctgta tatgcctttg tccatcaaaa
    10501 gaaaaagcat ctcttgacct tctggtggca atctgtgtat ttcatctaag aataatatgc
    10561 ctccatcagc ctgtgcaact atgccgaatt tatccttatt agcaccagta aaagaacctt
    10621 tgacataacc aaataaatta cttaataata agttagcatt ttctgcatat tcagcacagt
    10681 taaatactac gaatttgctt tcagaagaaa agattttact ttctacagca tatttgtaca
    10741 ttgtttcggc aaatgtactt tttccaacac cagtaggtcc tatgagtaat gtattaagac
    10801 cttttggagg atagagtata gcagatttag ctttttctat aggtagttta agactagaat
    10861 catgtccaag taatgttgaa aaaatatctt ctgagtcatt tttacttgat ttaagtagct
    10921 cattacaatt atttatagaa aattcatcag tgttcaaagt acggccaatt aaattttcta
    10981 taatactagc atgtagaaat aaaattggct taccagatat tttcagaact tttttttcat
    11041 tgtataggtt atttaattct ttactggtat tacttctatc aagagatgaa tggttagcaa
    11101 tttctattgt agtgaatcct gttttatctg attgaaggtc ttttaaagtc atattttcac
    11161 ataatttatg tagagataat agaactttat cttttcttag catataccgt ttcctttctt
    11221 tatgtttaaa ctatatttaa gctacattaa atatattata atatatattc agtattaaaa
    11281 taaggtttat agtggatatt ttgtatttta ctttttaata tgttgttttg tgtaatagat
    11341 ggtgttaaaa taagagttgc attatattag gtatggattt ttatagatta aattagagta
    11401 ttatgttgtt aaataactaa atttaagttt tttaatatac ttaagtctat gtaatatcat
    11461 ttattaatat atggtcggta tagatgaa
//
LOCUS       DBGJFF010000121        11455 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.123, whole genome shotgun sequence.
ACCESSION   DBGJFF010000121 DBGJFF010000000
VERSION     DBGJFF010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11455
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.123"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            <1..781
                     /locus_tag="ACOIAF_003065"
     CDS             <1..781
                     /locus_tag="ACOIAF_003065"
                     /inference="COORDINATES: protein motif:HMM:NF013122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HGZ2670066.1"
                     /translation="FMEYIIFDLEFNQGFDKKLNKTVSNEKCPFEIIQIGAIKLDSKF
                     NIIDTFNSYIKPTIYKDIHPFISRMTNIKNSDFNDSPTFPEVYNNFIKFISSQDPILC
                     VWGAGDLKELYRNINYHKLPSNSLPKSYINIQQHASKYFNNPAGKSIGLQNAISILEL
                     DEKMSYHNALNDAYYTAKVFIKIYNPSIVPDIYLYTSIKPKTIRYSNKKRVDYDKLFD
                     EFRKILNRELTKDEKKIINLAYNMGKTNQFTLENVKQRKNK"
     gene            complement(873..1625)
                     /locus_tag="ACOIAF_003066"
     CDS             complement(873..1625)
                     /locus_tag="ACOIAF_003066"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HGZ2670067.1"
                     /translation="MKFISWNVNGIRACVGKGFLDFFKEVDADIFCLQETKLQEGQIE
                     LDLPGYFQYWNYAERKGYSGTAIFTKKEPLKVMYGINIEEHDKEGRVITLEFEDFYFV
                     TVYTPNSQSELKRLEYRTRWEDDFIDYLTKLDNHKPVIVCGDMNVAHKEIDLKNPKNN
                     MKNAGFTKEEREKFSKLLDSGFIDTYRYFNPDKEGVYSWWSYRFNARKNNAGWRIDYF
                     CASKKLEDRLVSADIHTEILGSDHCPVELEIK"
     gene            complement(1786..2736)
                     /gene="galT"
                     /locus_tag="ACOIAF_003067"
     CDS             complement(1786..2736)
                     /gene="galT"
                     /locus_tag="ACOIAF_003067"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HGZ2670068.1"
                     /translation="MKELRIDPITNDVVIFATDRLKRPLDTADIPNEDEETNEYDEEC
                     PFCKGNEAHATDALFEIDSEEGWLVKSVYNKFPIIDDMARDVYGVHEVMIESDKHNRS
                     FYNMSQKEFEDVFFMYRNRFRDLSKDDKVEYVSIFKNFLRKAGASLMHPHAQILSMSF
                     IPPEITNELLVSKEYYDSNKSSLYDDLIENEISLDRRVVYNGEAFLVLIPYATKYSGE
                     VRIIFKDKIEFGELNDNNIQELSTIFEKLFKKLYNINGYMPFNLCIHTHPTKIETKSY
                     FNVHMHIIPRKYNFGGFELGTNMYVSSMSPEDLTKKLKID"
     gene            3121..4317
                     /locus_tag="ACOIAF_003068"
     CDS             3121..4317
                     /locus_tag="ACOIAF_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HGZ2670069.1"
                     /translation="MNYSNRVSAMQASPIRKLVPFAQAAKDKGIKVYHLNIGQPDIKT
                     PKGFFDAVKNFDSEVLEYATSEGIPELLEALQNYYKTYDMNFEKDELLVTNGGSEALL
                     FTMMAVCDPGDNLLVPEPFYTNYNGFGQSVNVEVNAVTTKAENGFHLPSKEEILSKVD
                     DKTKAIILSNPGNPTGAIYTKEELNILAEIAKEKDLWIIADEVYREFVYDGLEYTSCG
                     NLEGVQDRVIIIDSVSKRYSACGARIGSIACKNKGLIAQILKLCQGRLCVPTLEQIGA
                     VELYKTPVSYFKEVNEEYKKRRDVLYNELMKVEGVICKKPTGAFYIVAKLPVENAEDF
                     TIWMLKEFNKDNETVMVCPAEGFYATPGLGRDEIRLAYILNEKDLHRAATLLKEGLEQ
                     YVALQK"
     gene            complement(4652..5104)
                     /locus_tag="ACOIAF_003069"
     CDS             complement(4652..5104)
                     /locus_tag="ACOIAF_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HGZ2670070.1"
                     /translation="MNYIVKDTISFLVKTFPKIYSSLYLEDLKKFAPDYNVNKTQLRA
                     LVFIKNYGVISMTDLCSKLNIEKGSLTSMVDDLTDKKYVTRKRDLVDRRKYLIDITEE
                     GDKIATDFMDKLSDGLGEKLSKLTEEDRKKYLEAINTLQYILNKEEFR"
     gene            complement(5415..6860)
                     /gene="purB"
                     /locus_tag="ACOIAF_003070"
     CDS             complement(5415..6860)
                     /gene="purB"
                     /locus_tag="ACOIAF_003070"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HGZ2670071.1"
                     /translation="MIDNKYTTYSNPLTDRYCSKDMSYIFSPQFKFSTWRRLWVALAE
                     GEHELGINITEEQLDELRANIDNINFDEARKIEKEVRHDVMSHVKAYGLQCEKAKGII
                     HLGATSAYVGDNTDVIQMNEALKLLRVKLVNLINNLKEFALKYKDMPALGFTHFQAAQ
                     LTTVGKRACLWAQDLILDLEDLNYRIDNMKLRGVKGTTGTQASFLSLFEGDHEKVKEL
                     DKKICKKMGYHSSYAVSGQTYTRKLDYQVISILSGIAQSMHKMTNDIRLLQSLKELEE
                     PFEKNQIGSSAMAYKRNPMRSERIASLSKYIISESISPAMVQATQWLERTLDDSANKR
                     LAIPQAFMAADAILEIGINVTDGLVVYENMINRRVNEELPFMATETILMEAVKRGGDR
                     QELHEIIREYSMKAAYRVKHEGKDNNLIELIMNDDSFKMSKEEILSIMDPKNFIGRAP
                     EQVVEFVNEVVEPAIKDYKEDLGKVDVDLRV"
     gene            complement(7004..7912)
                     /locus_tag="ACOIAF_003071"
     CDS             complement(7004..7912)
                     /locus_tag="ACOIAF_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGZ2670072.1"
                     /translation="MYSTKILDDYEYETFNVFSTKLNPNTKHDYLSKVILFKEFLKGK
                     ELIYATKEDCKNFVDYIQTKYAKSTCEKIYSYLHSFYNFLKKEGYIDINPFRYVEKPT
                     VTRIKTKDDVLSIQEINKLIGILPKLNIRDRVIIVCLVTTGCLLNELVSLKWKDLMVD
                     ENDNSYVRLGKGRKERVVKLHPYFFKLLEDYRNYSGLPEVIIPSDDFIFTTQKSNSIT
                     DRNVRLIVKKALDLAGLSQYSARDFRHSFAAISLRLGADESDVKNQLGWSDKYYAIRY
                     KYVLNFVDSEIVDYLIEKDNLDINKK"
     gene            8169..8993
                     /locus_tag="ACOIAF_003072"
     CDS             8169..8993
                     /locus_tag="ACOIAF_003072"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HGZ2670073.1"
                     /translation="METFSLLEIFKAVILGIVQGITEWLPVSSTGHMILVDEFIKLNF
                     SNTFISTFLVVIQFGSILAVLVIFFRKLNPFDSAKNIKQKKETVRLWLKVIIAVIPSG
                     VIGILFEDDIDRLFFNSTVVAIALIVYGIIMIGLEKRNKKPKYKDFSQVTYKLALCIG
                     LFQCLALIPGTSRSGSTIIGAVLLGTSRYVAAEFSFFLAIPTMLGASALKLLKAGFGF
                     TGFEWLILGVGSVVAFVVSIVVIKFFMDYIKKHDFKVFGYYRIVLGIVVLAYFFLL"
     gene            9033..9563
                     /gene="lepB"
                     /locus_tag="ACOIAF_003073"
     CDS             9033..9563
                     /gene="lepB"
                     /locus_tag="ACOIAF_003073"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HGZ2670074.1"
                     /translation="MSVKKEIFDWIKSIAMAIVLAFVILQFIRPSIVSGESMYPTLDD
                     KDYLILNRISYKVGKPEKGDIVVFKTNLVDGETGKKKDLIKRVIATEGDRIKISNSKV
                     YVNGKLLNEPYIHNNYTSGDIDTVVPKGKLFAMGDNRENSNDSRFPDVGMVDEDEVLG
                     KVMVRLLPLDNIGKVD"
     gene            complement(9645..11426)
                     /locus_tag="ACOIAF_003074"
     CDS             complement(9645..11426)
                     /locus_tag="ACOIAF_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HGZ2670075.1"
                     /translation="MSQYININSQLDALKLKEFEKIFYENINEDVKVVPKVTPFKGIN
                     TDLLYVKDGRILFIKFMDTTEDIFFILEEELLEVMNEEYELLKLKMGQKNRNISYNYV
                     YIMPYVEVEETYEFEEFVNNNIIDKNKLQDIMNKGSLEEYLNDENDEINLNLFLLDIC
                     SEYYIINDKLHLNEKFKKISFYNDDYKYTATMMEDVQIKDVVSIKYGNTLIEGGSGIG
                     KTAIMLSRAIKLAKVYPHHKLVIFTHTKQLCNELRERIELLYKDNNNLEVHTFSSFIF
                     KLAKKFNLIVDYNMLKNDYEKTFNNLVKQAQNIIKNKNMFKAIFIDEAESFLEYEIDF
                     IREFLYKTKFIFNVCSCNSLNISNRLNIFKKLYNGIEFDDKIILSKNYRQAKEIVDFT
                     NKFSNNSNSYINELRPNTEFSTFFYTKALRGGNKSVDIIKVSDLDDQISSVIWEIEYL
                     ISKKGLDYSEVAIVYPYNKKKLKSGKTIYFQYMLKKALEEAKIPYICAEDNLTNISKK
                     VGTTIANIYAIKNLEYKAVIVCELEMLYNQTINDIEQDYQVNDFVGDLNKVYLAMSRA
                     TDYLSIVTTFNEEASDIIRLITESKDI"
BASE COUNT         3838 a         1734 c         1396 g         4487 t
ORIGIN      
        1 ctttatggaa tacataattt tcgatttgga atttaaccaa ggatttgata aaaaactcaa
       61 taaaactgta tccaatgaaa agtgtccttt tgaaattata caaataggag caattaaatt
      121 ggattctaaa ttcaatataa tagatacgtt taatagctat ataaaaccta caatttataa
      181 agacatacat ccctttatta gtcgtatgac taatattaaa aattctgatt ttaatgattc
      241 gcctactttc cctgaggtct acaacaattt tataaaattt atatcatcac aagaccctat
      301 attatgtgtt tggggagctg gagatttaaa ggaattatac aggaatatca actaccacaa
      361 gctaccatca aattccttac ctaaatctta tattaatata caacaacatg catctaaata
      421 tttcaataat ccagcaggaa aaagcattgg cctacaaaat gctatttcta tattagaact
      481 tgatgaaaaa atgtcttatc acaatgcttt aaatgatgca tattatactg ctaaagtttt
      541 tattaagata tacaatccaa gtatagtccc agatatatat ctttatacaa gtataaaacc
      601 taaaactata agatattcta acaaaaaaag agttgattat gataagttat ttgatgaatt
      661 taggaaaatt ttaaatcgtg aattaactaa agacgagaaa aaaatcatta acttagctta
      721 taatatgggg aaaacaaatc aatttacatt agaaaatgtt aagcaaagaa aaaacaaata
      781 gagtttattt agataaaaaa taggcatgta gattaaaaaa ctagttaact ttaaatttat
      841 atctacagcc caatttttat cataattaat ttttatttta tctctaattc aactggacaa
      901 tggtctgaac ctaatatttc agtgtgtata tccgcactta ctaatctatc ttctaatttt
      961 ttggatgcac aaaaataatc tattctccac cctgcattat tttttcttgc attaaatcta
     1021 tatgaccacc aactataaac accttcttta tcaggattaa aatatctata tgtgtctata
     1081 aacccactgt ctaataattt agaaaacttc tctctttctt ctttagtaaa acctgcattt
     1141 ttcatattat ttttaggatt ttttaaatct atttctttat gtgcaacgtt catatctcca
     1201 cacacaatta caggcttatg attatctagt ttggttaaat aatctataaa gtcgtcttcc
     1261 catctagttc tatattctag tctctttaat tcagattgag aatttggcgt atatactgta
     1321 acaaagtaaa aatcttcaaa ctctagagtt atcactctac cctctttgtc atgttcttct
     1381 atatttattc catacattac ttttaaaggc tctttttttg taaatatagc agtacctgaa
     1441 taaccctttc tctcagcata attccaatat tgaaaatatc ctggtaaatc taactctatt
     1501 tgcccttctt gcaatttagt ttcttgtaaa caaaatatat ctgcatctac ttctttgaaa
     1561 aagtctaaaa atcctttccc aacacaagct cttattccat taacattcca tgatataaat
     1621 ttcatactta tactcctcat ctaaacattg atacactccc catagctaaa gatgggggct
     1681 tttcaacaat tttattgtaa attcttaatt cacttttatt ttaacaatgc tatactataa
     1741 tttcatctaa aattactatt tacttaatac ctaaaaatat atgttttaat caatttttaa
     1801 cttttttgtt aaatcttcag ggctcattga agaaacatac atatttgttc caagttcaaa
     1861 tcctccaaaa ttatattttc ttgggattat gtgcatatga acattaaaat acgattttgt
     1921 ttcaatcttt gttggatgag tgtgaataca taagttaaat ggcatatatc cattgatatt
     1981 gtataacttc ttaaacaact tttcaaatat tgtagaaagc tcctgtatat tattatcatt
     2041 taactcacca aactctattt tatctttaaa tattattctt acttctccac tatacttggt
     2101 tgcatatggt ataagtacca aaaaagcttc tccattataa acaactcttc tatctaaact
     2161 tatttcattt tctattaaat catcatacaa agagctttta ttgctgtcat aatactcctt
     2221 tgaaaccaac agttcattcg taatttcagg tggaataaaa gacatagaca atatttgagc
     2281 atgtggatgc atcaaagatg ctcctgcctt tctcaagaag tttttgaaga tactaacata
     2341 ctctacttta tcatctttcg acaaatctct aaatctattt ctatacataa aaaaaacatc
     2401 ttcaaattct ttttgactca tgttataaaa actacgatta tgtttatcgc tctctatcat
     2461 tacttcatga acaccatata catctcttgc catatcatca attataggaa atttattgta
     2521 gacagatttt acaagccatc cttcttcact atctatttca aataatgcat cagttgcatg
     2581 agcttcattt cctttgcaaa atgggcattc ttcatcatat tcattagttt cttcatcttc
     2641 atttgggata tctgctgtat ctaaaggtct ttttaaccta tctgtagcaa atataacaac
     2701 atcattggta attggatcta ttcttaattc tttcataatt aacacctctt agttaatagc
     2761 ctttaatata attatatgat atttatcgta tctgttaaat aaatttataa tattttttat
     2821 aattttgcat atttattatt atatattttg tctatagctg aaagattata actaaaaaat
     2881 cactatcagt atatcagaat attatcttac tataaattaa tttatgaata cataatttag
     2941 tggcataaac atgggtattt tactataatt tgttaaaagt ttattttaaa gctatattaa
     3001 ttttttttga tgatttttac ccataagtat gttattatta tcttgtaaag atgtcttgta
     3061 ttcaaacata caatttacat aatacaatat acgtatttaa attaaatagg gggtattaaa
     3121 atgaattatt caaacagagt tagtgcaatg caagcctctc caatcagaaa acttgttcca
     3181 tttgcacaag cagcaaaaga taaaggaatc aaagtatatc acttaaacat aggacaacca
     3241 gacataaaaa ctcctaaagg attttttgat gctgttaaaa actttgatag cgaagtctta
     3301 gaatatgcta catcagaagg tattccagaa ttattagaag cacttcaaaa ttactataaa
     3361 acttacgata tgaattttga aaaagatgaa ttattagtaa ctaatggtgg tagtgaagct
     3421 ttacttttca ctatgatggc agtttgtgac ccaggagata atctattagt tccagaacca
     3481 ttctatacaa actacaatgg atttggtcaa tctgtaaacg tagaagttaa tgctgttaca
     3541 acaaaggcag aaaacggttt ccaccttcca tctaaagaag aaatattgtc taaagtagat
     3601 gataaaacta aggctataat tctttctaac ccaggaaatc caactggtgc aatttataca
     3661 aaagaagaac ttaatatcct agctgaaata gctaaagaaa aagatttatg gataatagca
     3721 gacgaagttt atagagaatt tgtttatgat gggcttgagt atacaagctg tggtaactta
     3781 gagggtgtac aggatagagt tataataata gacagtgttt ctaagagata cagtgcttgt
     3841 ggtgctagaa ttggttctat cgcttgcaaa aataaaggtc ttatcgctca aatacttaaa
     3901 ttatgtcaag gaagactttg tgttcctaca ttagagcaaa taggagctgt tgagttatac
     3961 aaaactcctg tttcttactt caaagaagtt aatgaagagt acaaaaaaag aagagatgta
     4021 ttatacaatg aattaatgaa ggttgaaggt gttatatgta aaaaaccaac tggtgctttc
     4081 tatatagttg ctaaattacc agttgaaaat gcagaagatt tcacaatttg gatgttaaaa
     4141 gaatttaaca aagataatga aacagttatg gtttgccctg ctgaaggatt ctatgctact
     4201 ccaggtttag gacgtgatga aataagatta gcttacatct taaatgaaaa agaccttcat
     4261 agagctgcta cattattaaa agaaggttta gagcaatatg tagctttaca aaaataattt
     4321 tttaaactta atttatataa atttaatgat ataaaatgtc cccttttcag atattatttg
     4381 aaaaggggac atttcttaat taatatatta attaagactt agacctgact atttttcatt
     4441 ttctttatat agtgttggcc agtaatcttt attcacttta attaaagcat ctaaaacttg
     4501 tcttgcctta gttagagaaa ttataaaaaa gtacatcaag aaaatttaag atgtactttt
     4561 tagattgtat ttagacttat aattatatag ttaaatgaat aacaaagaat taatcttagt
     4621 accattaaat acaaaaattt ttatgttttt tctatctaaa ttcttctttg tttaatatat
     4681 attgaagagt atttattgct tctaaatatt tttttctgtc ttcttcagtt aatttagaaa
     4741 gtttttctcc taaaccatca cttaatttat ccataaaatc agttgctatc ttatctccct
     4801 cttcagtaat gtcaattaga tattttcttc tatcaactaa atctcttttc cttgttacat
     4861 acttcttatc agttaaatca tcaaccatac tagttaaact gcctttttct atatttaact
     4921 tagagcataa atcagtcata cttattactc cataattttt tatgaaaact aatgctctta
     4981 attgtgtttt atttacatta taatctggtg caaatttttt taggtcttct aagtacaggc
     5041 tagaataaat cttaggaaat gtttttacta aaaaacttat ggtatctttt actatatagt
     5101 tcatataatc acctcggttg ttttaaaatt tattaaatta tctaaataac tttatttcag
     5161 tttacattaa aaatattgca agtacaatag tttttgctga atgtttaaat ttaattagta
     5221 atcttatcat ttttttattt ttatttatat tattcctatt aaatattatt tatatagcta
     5281 ttttattaaa ttcatggtat agtaagtaaa gaagaactct tcacagcggt ggagagaaga
     5341 cgctagccca ttaaaatggc tatcttattt ataaataaga tagccgtttt taattatatt
     5401 ttatctgtac aattctatac tcttaggtct acatctactt ttcctaagtc ttccttataa
     5461 tcttttatag caggttctac aacttcattt acaaattcta caacttgttc tggagctcta
     5521 cctataaagt tttttgggtc cattatagat agtatttctt ccttgctcat tttaaatgaa
     5581 tcatcattca ttataagttc aattaagtta ttatccttgc cttcatgctt tactctatag
     5641 gcagctttca tagaatattc tcttattatt tcatgtaatt cctgtctatc tccacctctt
     5701 tttacagctt ccataagtat tgtttctgta gccataaaag gaagttcttc atttacacgt
     5761 ctatttatca tattttcata tactactaaa ccatcagtta catttatccc tatttcaagt
     5821 atagcatctg ctgccataaa tgcttgaggt attgccaatc tcttattggc tgagtcatct
     5881 aatgttctct ctaaccattg agtggcttgt accattgcag ggcttataga ttcagagatt
     5941 atatacttag ataaagatgc tattctctca cttctcattg gattcctctt ataagccatt
     6001 gctgatgaac ctatttgatt tttttcaaat ggctcttcta attcttttaa actttgtaaa
     6061 agtcttatat cattagtcat cttatgcata ctttgagcta tccctgatag tatgcttatt
     6121 acttgataat ctaactttct agtatatgtc tgtccactta ctgcatagga agaatggtat
     6181 cccatctttt tacaaatttt cttatctaac tcttttactt tttcgtggtc tccttcaaat
     6241 aagcttaaaa aacttgcttg agtaccagta gtacctttaa ctcctcttaa cttcatgtta
     6301 tctattctat agtttaaatc ttccaaatca agaattaaat cttgagccca taaacaagct
     6361 ctttttccta ctgttgtaag ttgagcagct tggaagtgtg taaatccaag agctggcata
     6421 tctttgtact taagtgcaaa ttcttttaaa ttgtttatta aatttacaag ctttactctt
     6481 agaagtttta aagcttcatt catttgtata acatctgtat tatctccaac atatgcagat
     6541 gttgctccta aatgtattat tcctttggct ttttcacatt gtaaaccata agcttttacg
     6601 tgagacatta catcatgtct tacttctttt tctatttttc tagcttcatc aaaatttata
     6661 ttatctatat tagctcttag ctcatctaat tgttcttcag ttatattgat tcctaactca
     6721 tgttcacctt ctgcaagtgc aacccataac cttctccatg tagagaattt aaattgtgga
     6781 gaaaatatat aactcatgtc cttgctacaa tatctgtctg taagtgggtt tgagtaagta
     6841 gtatatttat tatctatcat tatattagca ccttccttaa atcttttgtc gtgtgattac
     6901 tgtgtacaca ttagtacata aataattata tataatttta cgaactataa caagactttt
     6961 ttattttttg ttcacattta tatatagtat tttttattta ttactatttt ttatttatat
     7021 ccaaattatc tttttcaatt aagtaatcca ctatttcact atcaacaaaa tttaatacat
     7081 acttatatct aattgcatag tatttatcac tccatccaag ctgatttttt acatcacttt
     7141 catctgcccc aagccttaaa cttattgctg caaatgaatg tctaaaatct cttgctgaat
     7201 attgagaaag tccagctaaa tctaatgctt ttttgactat aagtctgaca tttctatctg
     7261 ttatagaatt actcttttga gttgtaaaaa taaaatcatc actaggtatt attacctctg
     7321 gaagcccaga ataattcctg taatcttcta aaagtttaaa aaagtatgga tgtaatttaa
     7381 ctactctttc ttttcttccc ttacctaacc ttacatatga gttatcgttt tcatctacca
     7441 taaggtcttt ccactttaag ctaactaatt catttaataa acacccagta gtaactagac
     7501 agactattat tactctatct cttatattta attttggtag gatacctata agtttattta
     7561 tttcttgtat acttagaaca tcatcttttg tttttattct tgttactgtg ggtttttcta
     7621 catatctaaa tggatttata tctatgtatc cttctttttt taagaagtta taaaaactat
     7681 gtaaataact atatatcttt tcacaagttg atttcgcata tttagtctgt atatagtcta
     7741 caaaattttt acaatcttct ttcgtagcat aaatcaattc tttccctttt aaaaattctt
     7801 taaacagtat aacctttgat aaataatcgt gtttcgtgtt tggatttaat tttgtagaaa
     7861 aaacattaaa agtttcatac tcataatcat ctagtatttt tgtactatac ataataactc
     7921 ctttcctttt cataaaataa aatagctata cttttactat accatatatt tccccctcaa
     7981 atatacccct tattcaatag atgactttta agtttatttt gttatattat gaaggtttta
     8041 tgaaaatatc aatcttccat tgtgcactac acagtactat ggtattataa gttagtaaaa
     8101 ggtggcattt ttttattaat ctatgaataa aatatgctac tataaatttg tattggaggt
     8161 attttgattt ggaaacattt agtttgcttg aaatttttaa ggcagtcatt ttgggcatag
     8221 ttcagggaat aacagaatgg ctaccagtaa gtagtacagg gcatatgata ttagttgatg
     8281 agtttataaa attgaatttt tcaaatacat ttataagtac atttttagtt gtaattcaat
     8341 ttggttcaat attagctgta cttgttatat tttttagaaa attaaatcct tttgatagcg
     8401 caaagaatat taagcagaaa aaagaaacag tccgtttatg gcttaaggtt ataattgcag
     8461 ttataccatc tggagttatt ggaatactgt ttgaagatga tatagataga ttattcttta
     8521 attcaacagt agttgctatt gctcttattg tatatggtat aataatgata gggcttgaaa
     8581 agagaaataa aaaacctaag tataaagatt tttctcaagt tacatataaa ttagctcttt
     8641 gtataggatt attccagtgt ctagctctta taccaggtac atctaggtct ggttctacta
     8701 taataggtgc agtattactt ggtacttcta gatatgtagc agcagagttt tcattcttct
     8761 tagccatccc tacaatgctt ggagctagtg cattaaaact tctaaaagct ggatttggat
     8821 ttactggatt tgaatggctt attttaggtg ttggttctgt agtagcattt gttgtttcaa
     8881 ttgtagttat taaatttttt atggactata tcaaaaaaca tgacttcaaa gtatttggtt
     8941 attatagaat agtcttgggt atagtggttc ttgcttattt ctttctatta taatgtattc
     9001 taatttaata acaaatgata aggagaaaag ttatgagtgt taaaaaagaa atatttgatt
     9061 ggattaagtc aatagctatg gctattgtac ttgcatttgt aattctacaa tttataagac
     9121 cttctattgt aagtggagaa tcaatgtatc ctactttaga tgataaagat tatctgattt
     9181 taaataggat atcatacaag gttggtaaac ctgaaaaagg cgatattgta gtttttaaaa
     9241 ccaatttagt tgatggagaa acaggaaaga aaaaagactt aataaaaaga gttatagcta
     9301 ctgaaggtga cagaataaaa atatcaaatt ctaaagtgta tgtaaatgga aaattattaa
     9361 atgaaccata tatacacaat aactatactt ctggagatat agatactgtt gttccaaaag
     9421 gtaaactatt tgcaatggga gataatagag aaaatagtaa tgatagtaga ttccctgatg
     9481 taggtatggt tgatgaagat gaagttcttg gtaaggttat ggtgagacta ttacctcttg
     9541 ataatattgg aaaagtagac taaaaaataa gagactgaat tggtaaagtt tattttacaa
     9601 atctcagtct ctttttattt tcttaaatta caaatatttt tttactaaat atcttttgat
     9661 tctgtaataa gtcttattat atcacttgct tcttcattaa aagttgttac tatacttagg
     9721 tagtctgttg ctctactcat agctaaataa accttattta aatctcctac aaagtcattt
     9781 acttgataat cttgttcaat atcatttata gtttgattgt ataacatctc taattcacat
     9841 actattaccg ctttatactc taaattcttt attgcatata tatttgctat tgtagttcct
     9901 accttcttac ttatatttgt caaattatct tctgcacata tataaggaat ttttgcctct
     9961 tccaatgctt tttttaacat atattgaaag taaatggttt ttccactttt taatttcttc
    10021 ttattgtaag gataaacgat agcaacttca gaataatcaa gacctttttt acttattaaa
    10081 tactcaattt cccagattac agaacttatt tggtcatcaa ggtcacttac ttttattata
    10141 tcaacagact tatttccacc tctaagtgct ttagtataaa agaaagtact aaattctgta
    10201 tttggtctta attcgttgat atatgagtta gaattatttg aaaatttatt tgtaaaatct
    10261 acaatctctt tagcttgtct ataattttta cttaagataa tcttatcatc aaattctata
    10321 ccattgtata attttttaaa tatattcaat ctattagaga tattcaagga gttacaagaa
    10381 cacacattaa atatgaattt tgttttatat aaaaactccc ttataaaatc tatttcatac
    10441 tctaaaaagc tttctgcttc atcaataaaa atagccttga acatattttt attttttata
    10501 atgttctgtg cttgttttac aagattatta aaggtctttt cgtaatcatt ttttaacatg
    10561 ttataatcta caattaaatt gaatttttta gctagtttaa atataaaaga actaaatgtg
    10621 tgaacttcta aattgttatt atctttgtat aataactcta tcctctctcg aagctcattg
    10681 cacaattgtt ttgtatgtgt aaatatgact aatttatgat gtggatatac ttttgctaat
    10741 tttatagccc tagaaagcat aattgctgtt ttgcctatac cagaaccacc ttcaataagt
    10801 gtattcccat atttaataga aacaacatct ttaatttgaa cgtcttccat catagttgct
    10861 gtatacttat agtcatcatt gtagaatgaa atttttttaa atttttcatt tagatgaagt
    10921 ttatcattta ttatatagta ttctgaacat atgtctagta aaaataaatt taagtttatt
    10981 tcatcattct catcattaag atattcctcc agcgaaccct tattcattat atcttgaagc
    11041 ttatttttat ctattatatt attattaaca aattcttcaa attcatatgt ttcctcaact
    11101 tcaacgtatg gcataatgta aacgtagtta taacttatat ttctattctt ttgtcccatt
    11161 tttagtttca aaagttcata ttcctcattc ataacttcaa gtaactcttc ttctaaaata
    11221 aaaaatatat cttctgttgt atccataaat tttataaata aaattcttcc atctttaaca
    11281 tacaacaaat cggtatttat tcctttaaaa ggagtaactt ttggcactac tttaacgtcc
    11341 tcattgatat tttcgtaaaa tattttctcg aactccttca attttagggc gtctaactga
    11401 gaatttatat ttatgtattg gctcacttac attacctcct tctgttatac ttaat
//
LOCUS       DBGJFF010000122        11304 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.124, whole genome shotgun sequence.
ACCESSION   DBGJFF010000122 DBGJFF010000000
VERSION     DBGJFF010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11304
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.124"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(406..1113)
                     /locus_tag="ACOIAF_003075"
     CDS             complement(406..1113)
                     /locus_tag="ACOIAF_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HGZ2670076.1"
                     /translation="MIFTKSNKYDKDFLMKNMMGPNCIKILEELTSKIKLEKGMRILD
                     LGCGKGISSIFLAKEFDATVFATDLWIEPTENYKRFKEFKLDDKIFPIQAEAHELPYA
                     EGFFDAVISIDSYHYFGNKEGFLDNHISPLVKEGGILAMAMPGLKEDFVDCIPDELIP
                     FWQDNMNFHSITWWNKLWSESESVIVEKCEALNCHDEAWKDWINCDNSYAINDKKMME
                     VENGKYFNTISLIARTK"
     gene            complement(1917..2702)
                     /locus_tag="ACOIAF_003076"
     CDS             complement(1917..2702)
                     /locus_tag="ACOIAF_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HGZ2670077.1"
                     /translation="MNFENDFITSEFRKKYIGLVLDNAQNINKVYTTTFPDKEIIKQV
                     IDKDEKDILIFSHHAMGYIASDEGFPFHDIPLSYMEEMKNRRISFYVLHSPLDNYSDY
                     STSVSFAKAMGLEIVKPFCKYDDKIEVGVICKTSLKSIEEIKELIKKSVGHDVKLYDY
                     GDSVLKDGLVAIAAGGGSYPFVAREVAELGINLYITGFTKPLKHFEPVLEFHQIAKDN
                     LINVIGATHYSTEKFACMGMVDYFKNLGLESEFLQGKYYLEDL"
     gene            complement(3091..3561)
                     /locus_tag="ACOIAF_003077"
     CDS             complement(3091..3561)
                     /locus_tag="ACOIAF_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGZ2670078.1"
                     /translation="MKYQVSLLAVKDIEVSKKFYKELFEQEIELDLGKNVTFKGGFAI
                     QEDFAWLTGINPDTVIQKSNNMELYFEVDDFDEFIEKLNYYKDVEFVHKPLKHEWQQR
                     VVRIYDPDKHIIEIGESMEVIARRYLSEGYSVEETSKIIQHPIEFVKQCENSKE"
     gene            complement(4295..4684)
                     /locus_tag="ACOIAF_003078"
     CDS             complement(4295..4684)
                     /locus_tag="ACOIAF_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HGZ2670079.1"
                     /translation="MTIKKLPQSELKIMKFIWKSDSKVTSRDIVLGMEQKYQWKQTTT
                     LTLLSRLVVKRFLNSQKIDKYTHYEVLIKEKEYIGVETRDFFRNIHDSSIKSLLLSLH
                     ENINLSKDDILLIEEWIKNLKEEEKDV"
     gene            complement(6444..7850)
                     /locus_tag="ACOIAF_003079"
     CDS             complement(6444..7850)
                     /locus_tag="ACOIAF_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HGZ2670080.1"
                     /translation="MDSVKYILRRFIGSSILISLLFIVLNIAGFLLFSYMLFVSKYSD
                     NLDTSDSREIKSNLIMIEDNLVLKDGKFILDDSSKEILHKNKVWAMLIDNNGDRIWGF
                     DLPKEIPSHYTLTDVAKFSRFFLKDYPVYVWEHPNGILVTGYPKDKYTKFNLTYTVKE
                     IEQFPMLIIGMFLVNAIVTFLIALFIGLKMVKSIKPIITGIKLMSKGEPVLLQEKGIL
                     SDISKSINTVSKELQMKDEKLRKKEEARSNWIAGISHDIRTPLSMIIGYAGELEESSS
                     LSNREQEQVSIICNQGIKIRELVNDLNLVSKLEYNMQALNCDKIRVSAFLRELISEFI
                     NNNLDNDFYVELDILNEDIIIDADKKLLKRAISNLIQNSITHNQQGCNIRVTSKSDLD
                     FCYIIVEDDGKGMSQERINSLLKSQNGLSEAYAKGQNHGLGILIVSGIINAHRGKLDI
                     TSIDSQGLKTILKLPLIK"
     gene            complement(7895..8602)
                     /locus_tag="ACOIAF_003080"
     CDS             complement(7895..8602)
                     /locus_tag="ACOIAF_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGZ2670081.1"
                     /translation="MESIKDKKILVVDDHKELLKMIDEILRKEGFSRVFLASSYEEAV
                     KVFRNVKPDCAILDVVLPDGDGFSIMRKIRETSKIPVIFLSARGEDEDRLIGLGLGAD
                     DYIVKPFLPKELTLRLVGILNRVYVPIEEEELPVFKLGDSVVVNLNSACVEKEGQEIS
                     LTAKEHSLLLKLYENKGRIVTNDSLCQAIWGDDMYGYENTLMVHVRRVREKIEKTPST
                     PKHLITVRGLGYKLMIN"
     gene            8764..9687
                     /locus_tag="ACOIAF_003081"
     CDS             8764..9687
                     /locus_tag="ACOIAF_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2670082.1"
                     /translation="MKHMEMIIRTQRLSKEYNKIFRVKDIDLRVPQGAVYGFLGPNGA
                     GKSTTLKMLLGLAKPTQGNINILGKELNEKNRISILKDIGSLIESPSYYGHLTGLENM
                     TIMQRLLDLPKKNVNEALKIVRLENQKNKKVSQYSLGMKQRLGIAMAIMKFPKLLILD
                     EPTNGLDPAGIEEIRELIKSLPEKYGMTVLISSHLLSEIDQIATSIGIINHGELVFQD
                     SIKELHNRGQSQIAIKTKNVVSAQKLLNTKGYMSLIQDGYLTIENLKDSDVAKINGML
                     VHSNIDVFRIEEHKKSLEDIFLELTGKAVTL"
     gene            9687..10448
                     /locus_tag="ACOIAF_003082"
     CDS             9687..10448
                     /locus_tag="ACOIAF_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2670083.1"
                     /translation="MKYLGLEFYKLKRKKIILMTFLFAAVEIMWCIVNSNRAITRNPD
                     MLGGFEYSYMLMSFASLNGLFFPILISIITSRISDIEHKGDTWKLLKSSVTSLNSIYL
                     SKFLCSAILVSIPVLMQVLSIVLFGHFRGVIESISVSLLLKYTLGTILVSIAIIALHL
                     WIATVIPNQMIAITFGMIGSFIGLTSGLFYQGIRRLFIWSYYLELSPLSYAYDNVLGS
                     SVYRIDMNFSITILVFLIGMLIYYIGRNHLLKKEV"
     gene            10460..11197
                     /locus_tag="ACOIAF_003083"
     CDS             10460..11197
                     /locus_tag="ACOIAF_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2670084.1"
                     /translation="MLRKSVLGEILKLKNSFIWYVLLALPLISVLIGSGNFYLNQGIL
                     KKEWYSLWTQVSLFYGEFFFPILIAIFCAYICRLEHMNHNWNNVMTLPVNLKNIFLSK
                     LTVVSILTAITQVFFVIFYIVAGNMFGFSGSVPKELTGWIAKGWIISISIASIQLYMS
                     IKIRSFATPIGISLCLSLFGMGLIVAKPAVGIFFPYSMLGSAMGVIKQSALNVNDTIV
                     TICVAFITTIVFSEIANRNFRKNDVIS"
BASE COUNT         3830 a         1833 c         1117 g         4524 t
ORIGIN      
        1 atatacttaa tcattttgaa atttgattta aagtataata aaatatataa aaaaatatat
       61 aaaatggtac tataaaattt ttagtcttca tcaatacctt ttttgctttc attgttaaag
      121 ctattttttc tattagttta tttcttataa aacaaacatt tagtattaaa atatatatca
      181 cacaatagta tttgcaattt aactttaaaa aaataaaaat acccaactga cctaagcaac
      241 tcgattgggt ataaataatc taaaaaatca tttttgtata aactctttat ttaacttaca
      301 tttttattta ccttctactt taattcaact tatatttcta tgcttcttat actctcattc
      361 aacttatatt tctacttatc ttatactttt ataaattcat cacaattatt tagttctcgc
      421 tatcaaagaa attgtattaa aatactttcc attctctact tccatcattt ttttgtcatt
      481 tatagcatat gaattatcac aatttatcca atccttccaa gcttcatcat gacaatttaa
      541 agcttcacat ttctctacaa tcacagactc cgattcagac caaagtttat tccaccatgt
      601 aattgaatgg aagttcatat tatcttgcca aaatggaatg agttcatctg gtatacaatc
      661 cacgaaatct tcttttaatc caggcattgc catagcaagt attcctccct cttttacaag
      721 aggtgatata tgattgtcta aaaatccttc tttgtttcca aagtagtgat aagaatcgat
      781 acttatcacc gcatcaaaga aaccttctgc ataaggtagt tcatgtgcct ctgcctgtat
      841 tggaaatatt ttatcatcca atttaaactc tttaaatctt ttatagtttt cagttggttc
      901 tatccataaa tctgttgcaa atacagtagc atcaaattcc tttgctaaaa atatcgaaga
      961 tataccctta ccacaaccta agtctaaaat acgcattccc ttttctagtt ttatcttact
     1021 tgttaattct tccaatattt ttatacaatt aggtcccatc atatttttca tgagaaagtc
     1081 cttatcgtat ttattacttt ttgtaaatat cattctatct ttcctttccc tatcagttat
     1141 tatttattaa attttatacg tttgattttt tattttttat atgcttctta tattgagcaa
     1201 tctctttttt attcattctt taacagacaa ttttaatgtg taattttatc cactctgtga
     1261 tagtaacttt aatgaataat cttatctact gtgatagtta actttgatgg gtaattttat
     1321 ctactccaca gcaatcgctt taatgagtaa tcttatccac tctatgatag ttattttagt
     1381 gagtaatttt atctactcta cagtaatcgc tttaatgagt aatcttatcc actctataat
     1441 agtcacttta atgagtaatc ataattttta tatcatttta gatacttact ctaataagaa
     1501 gtcattttct aatacttaat aagttttaat tacttccact ttaacctata tactaattaa
     1561 cttatttaat ttagtgcata tttgacactt taccttttat tataccattt attatcataa
     1621 atataataat tctatttttt taaaataaat atttataata atctaaaata aatatttata
     1681 aattctaatc aatcctaaat aataatctaa aatacatcaa aaaataaact aaaaaatatc
     1741 gaaaaataat ttaaaagaca tcaaaactaa atcttctaat cagcaattaa aagtctattt
     1801 tcttagttta aaaaataaag atagattttt tcaaaacact ggataaatag gtaaaaaaat
     1861 aaacagttag taaaataaat ttttactaac tgtttatttt tattgtttaa tatgtcctat
     1921 aaatcctcta agtaatattt tccttgcaaa aattctgatt ctaaacctag atttttaaaa
     1981 taatcgacca tgcccataca cgcaaatttt tctgtagaat aatgagtagc acctataaca
     2041 ttaattaaat tatcttttgc tatttgatga aactctaata ctggttcaaa atgttttaaa
     2101 ggcttagtaa acccagtaat atatagattt ataccaagtt cagcaacttc tcttgctaca
     2161 aatggataac ttcctccccc tgctgcaata gcaactaaac catcttttaa tacagaatct
     2221 ccataatcat acaatttcac atcatgacca acagattttt taattaattc cttaatctct
     2281 tctattgatt ttagtgatgt tttacatata actcctacct caattttatc gtcatattta
     2341 caaaatggtt tgactatttc taaacccata gcttttgcaa aactaacaga agttgaatag
     2401 tcactatagt tgtctagtgg agaatgtaat acataaaaag aaattctacg atttttcatc
     2461 tcttccatat aagatagtgg tatgtcgtga aatggaaaac cttcatctga tgctatgtat
     2521 cccattgcat gatgtgaaaa aattaaaata tctttttcat ctttatctat aacctgctta
     2581 attatttctt tatcaggaaa agtagttgtg tatactttat taatattttg agcattatct
     2641 aatactaatc ctatgtattt ttttctaaat tctgaagtga taaaatcatt ttcaaaattc
     2701 ataaaactcc aatcatcatt aatattttta atattaaatt ctaaatctaa ttttttataa
     2761 agttcaattg ccttaataat aaccactcct ttataataat attgaattac tacatattaa
     2821 tatagaaatt tcacgataat aaactttata cgtttaagta ttcctactta aaattaaaaa
     2881 tattataaat attcaaattt attaatatat tttaatatta tatttatttt ttctttatta
     2941 atattcccct tatttttatt tattttttct ttattaatat tttctttatt ttaatttaat
     3001 ttatgacatt tgtcaaatac tagtcctata tattatcttg aattagttct accacaatta
     3061 atttctacta aataattata atttaaacaa tcattcctta ctattttcac actgctttac
     3121 aaattcaatt ggatgttgaa ttattttaga agtttcttct acagaatatc cctcagataa
     3181 atatcttcta gctataactt ccatagactc tcctatctca ataatatgct tatctgggtc
     3241 ataaatacgc actactcttt gttgccattc atgttttagt ggcttatgga caaactctac
     3301 atctttataa taattgagtt tttcaataaa ttcgtcaaaa tcatcaacct caaaataaag
     3361 ttccatatta tttgattttt gtataactgt atctggatta atccctgtaa gccatgcgaa
     3421 gtcttcttgt atagcaaatc ctcctttaaa tgttacattt ttacctaagt ccaattcaat
     3481 ttcttgctca aataattctt tgtaaaattt ttttgatact tcaatatcct tcactgctaa
     3541 taatgaaacc tgatatttca taaattttcc tcctaatttt ttatttctat tttttcagta
     3601 tcttctatta acattttttt ctctatctct tcagtacctt ttattaatcc tttattttta
     3661 cttttttagt atcttctatt aacacttttg tttctacctc ttcagtatct tatatcaatc
     3721 ttttattttt tcaatatctt ctattaacac ttttatctct atctattcag tactttctat
     3781 caatcttttg tttttatttt ttcaatatct tctattaata cttttgtttc tacctcttca
     3841 gtatcttata tcaatctttt attttttcaa tatcttctgt taacactttt atctctatct
     3901 ctttaatatc ttatattaat cctttatttt tattttttca atatcatcat taattcctat
     3961 ttattttatc atcttaaata cgaaaacact gtgtcatatt tgcaaaatat atattatcat
     4021 ctatattaaa gcttcacaat ttatatttaa aaattctctt taattacact acctctattt
     4081 aaaaagctac cttaattata cttataagta aaggtagctt tttaaattta attaaataga
     4141 atatccacta ttaatttata tttaatttat ttagattcac attatagtat cataatccac
     4201 ttataatcat ttgtcatatt acttaagtag actaatatat tacataaata cacttacata
     4261 cactttatat tgatgctata agaaatacgt ttgcttatac gtctttctcc tcttctttta
     4321 gattttttat ccactcttca ataagtaaaa tatcatcttt gctcaaatta atattttcat
     4381 gtaatgacaa caataaactt tttatagaag aatcatgaat atttctaaaa aaatctcttg
     4441 tctcaactcc tatgtattcc ttttctttaa ttaaaacttc ataatgcgta tatttatcta
     4501 tcttttgaga atttagaaat ctttttacta ctaatctaga taaaagagtc aaagttgtcg
     4561 tttgtttcca ttgatatttt tgctccattc ctaaaacaat atctctagat gttactttgg
     4621 aatctgattt ccatataaat ttcattatct ttaattctga ttgtggcaac ttttttatag
     4681 tcatgataaa accttgctac tcagaatata gacaagcaat atccataata aaatttttcg
     4741 attaaataca tcttatacgg atttatataa gctcagactt ataagatatt tttcaatctt
     4801 aatcctaaat ttagctattt gtacacggta aatatatata aataatgtca aaattctctt
     4861 gtattatatt gcttaatttt atgaaatatt ttgtactcag ctatctattt tggatacttg
     4921 tccagtagct ctcctttctt ttaaactctt tgattttaaa tatttgtatt tcagtaaatc
     4981 ctacttccta aattttttat ttactacaac ttattactga acttcctact tcctaaactt
     5041 tttatttact acaacttatt actgaacttc tcacttacta aatttcttac ttactataaa
     5101 ttataactaa acttctcact tactaaattt cttacttact ataaattact actaaacttc
     5161 ctactttcta aatttcttac ttgctacaaa ttattactaa acttcctact ccctaaattt
     5221 cttacttgct ataaattatt actaaacttc ctactttcta aatttcttac ttactataaa
     5281 ttattactaa acttcctact ccctaatttt cttacttact ataaattatt actaaatttc
     5341 ctactttcta aatttcttac ttactacaaa ttattactaa acttcctact ccctaaattt
     5401 cttacttgct ataaattatt actaaacttc ctactcccta agtttcttac ttgctacaaa
     5461 ttattactaa acttcctact tcctaaattt cttacttgct ataaattatt actactaaac
     5521 ttcccactta ctaaatttct tacttactac tacttataac taaacttccc acttcctaaa
     5581 tttcttactt actaccactt atcactaaac ttcccacttc ctaaatttct tacttactac
     5641 cacttatcac taaacttccc acttcctaaa tttcttactt actaccactt atcactaaac
     5701 ttcctacttt ctaaatttct tgcttcttaa gaataatgtt aattttaatt ttaagattat
     5761 agtttttacc ttgtttaatt gaaacattct atataattat atccttctat tttagagttt
     5821 ttttatttat atattacaaa gtttaaattt tttcattaat tatattataa attttccata
     5881 tatttcaaca taaatacata tatatatatg ttatttctaa aaatactaca caaaaactct
     5941 actttagtag aacttaatgg cgcaaaatac ttcaatttaa aacatttatc gtactttaaa
     6001 taaataacta taataatata cttacaatag ttacatagaa gctattataa ctacacagaa
     6061 cagcgtataa ttaaaacttt tttaggatgt cattatattt tttcttaaaa ttaaaaaaca
     6121 ctataccata attctatttt agtagaattt ttttgtcaat aataaaatta aatattaact
     6181 aaaaaataaa aaaaatggaa ttatctaaag aatttaataa ccttacaata attccaaaat
     6241 attatttcta tgaaaatttt gataccatga gtaactcagc cataagcgca taaagcacaa
     6301 gttactcttt actttaataa aacttgacaa gaaaagaatt tatccttatt ctactctaat
     6361 agagttaatt tgtcaataaa gaataaaatt attttcaaaa tattttcatt cttcattctt
     6421 atctgttatt attttataaa atatcactta attagaggta attttagtat tgtttttagt
     6481 ccctgactat caatgctcgt aatatctaat ttacctctat gtgcattaat aatgccagat
     6541 actatcaaaa tacctagacc atgattttga cctttggcat atgcctcact tagtccattt
     6601 tggctcttta ataaactatt aattctttct tgactcatac cttttccatc atcttcaaca
     6661 attatatagc aaaaatctaa atcagactta gaggtaaccc ttatattaca tccttgttgg
     6721 ttatgagtta tactattttg tattaaatta ctaatagccc tttttaataa ttttttatct
     6781 gcatctataa taatatcttc atttagtata tcaagttcaa cgtaaaagtc attatccaaa
     6841 ttattattta taaactcaga aataagctct ctcaaaaacg cagaaactct aatcttatca
     6901 caattcaatg cttgcatatt atattcaagt ttagacacta aatttaggtc atttactaat
     6961 tctctgattt taatcccttg attgcatata atagacactt gctcttgttc tctatttgaa
     7021 aggctactgc tttcttcaag ttctccagca tatccaatta tcattgaaag tggagttctt
     7081 atatcatgag atattcctgc aatccagtta gacctagctt cctctttctt cctaagtttt
     7141 tcatctttca tttgcaactc tttagataca gtattaatag attttgaaat atcacttagt
     7201 attccttttt cttgtaaaag tactggctct ccttttgaca taagctttat gccagtaata
     7261 attggtttta tagatttcac catttttaaa cctatgaata gagctattaa aaaagtaact
     7321 attgcattaa ccaaaaacat acctattatt aacataggaa attgttctat ttccttaacc
     7381 gtataagtaa ggttaaactt agtgtacttg tcttttggat aacctgttac taatattcca
     7441 tttggatgtt cccatacata cacaggatag tcctttaaaa agaaccttga aaactttgct
     7501 acatctgtca aagtataatg acttggaatt tcttttggta aatcaaatcc ccatattcta
     7561 tctccattgt tatcaattaa cattgcccaa actttatttt tatgtagtat ttccttacta
     7621 gaatcatcta aaataaactt tccatctttt aaaaccaaat tatcttcaat cataatcaag
     7681 tttgatttta tttctctgct atcacttgta tctaaattat cagagtattt agatacaaat
     7741 aacatatatg aaaacagaag aaaacctgct atatttaata caataaaaag taatgaaatc
     7801 aagatacttg aacctataaa tcgcctcaat atgtacttta cactatccaa aattaaatca
     7861 ctcctcttga tttcttttat tctaatatct aaaattaatt aatcatcaac ttatatccaa
     7921 gacctcttac tgtaataaga tgtttaggtg tagatggagt tttttctatc ttctctctaa
     7981 cccttctaac atgaaccata agggtatttt catatccata catatcatct ccccaaatgg
     8041 cttgacatag tgaatcatta gtaacaattc tccctttatt ttcatacaac tttaaaagca
     8101 acgaatgctc tttagctgtt aaagaaatct cttgcccttc tttctcaaca catgcactat
     8161 ttaaattcac aacaacagaa tctcccagtt taaatactgg caactcctct tcttctatag
     8221 gcacatatac acgatttaat atacccacaa gccttaaagt aagttccttt ggtaaaaatg
     8281 gctttactat ataatcgtca gcccctaagc ccaaaccaat caatctatct tcatcttcac
     8341 ctcttgcaga gagaaaaatc actggtattt tagatgtctc tctaattttt ctcataatag
     8401 aaaaaccatc tccatctggc aatacaacat ctaaaattgc acaatctggc tttacatttc
     8461 taaatacctt aacagcttcc tcataacttg atgctaaaaa tactctacta aatccctctt
     8521 ttctcaggat ttcatctatc atttttaata attccttgtg gtcatcaacc acaagtattt
     8581 ttttatcttt tattgactcc ataatcatca ccctccttta gtataccata tacccatttt
     8641 cacaactttg cctaaataga cttttaaggt tattgtaagg taaatttcag ctaattgaaa
     8701 tgtaaccaag ttattatact gatataagtt aaaagaatca ataagcacag attaaaaaag
     8761 gggatgaaac atatggaaat gataattaga acacaaagac tttcaaagga atacaataaa
     8821 atatttagag taaaggatat agacctaaga gttccacaag gagctgtata tggttttttg
     8881 ggtcctaatg gagctggaaa atctaccact cttaaaatgc ttcttggact tgctaaaccc
     8941 acacaaggaa atattaatat tttaggtaaa gagttaaatg aaaaaaatag aatatcaatt
     9001 ttaaaagata taggttcttt gatagaatct ccatcatact atggtcatct tacagggctt
     9061 gaaaacatga ccataatgca aagactctta gatttaccta aaaaaaatgt aaatgaagca
     9121 ttaaaaattg taaggttaga aaatcaaaaa aataaaaaag ttagtcaata ttctttaggt
     9181 atgaagcaac gtcttggtat tgctatggca ataatgaagt ttcccaaatt attaatactt
     9241 gatgaaccta caaatggact cgacccagca gggattgagg aaattagaga attaataaaa
     9301 tctctaccag aaaaatacgg gatgactgtt ctaatctcta gccatctttt aagtgaaatt
     9361 gaccaaattg ccacatcaat aggaataata aatcatggcg aactggtttt tcaagatagc
     9421 ataaaagaac ttcataatag aggtcaaagt cagattgcta tcaaaactaa gaatgttgta
     9481 tctgcacaaa aactattaaa tactaaagga tatatgtcac ttatacaaga tgggtacttg
     9541 actattgaaa atttaaaaga ctcagatgtg gctaaaataa atggtatgct tgtccattca
     9601 aatatagatg tatttagaat agaagaacat aaaaaaagct tggaagatat attcttagaa
     9661 cttactggta aggcggtgac tttataatga aataccttgg tctagaattt tataaactta
     9721 aaagaaagaa gattattctg atgacctttt tatttgcagc tgttgaaata atgtggtgta
     9781 tcgtaaattc aaatagagct attacacgta atccagatat gttaggtgga tttgaatatt
     9841 cctatatgct tatgagtttt gcaagtttaa atggtctatt ctttcctatc ttaatatcaa
     9901 taatcacatc acgtataagc gatatagaac ataaaggaga tacatggaag cttttaaaat
     9961 cttctgtgac atctttaaat agtatttatt tatctaaatt tttatgttct gctattttgg
    10021 tatctatacc tgtattgatg caagtgttat ctattgtgtt gtttggacat tttagagggg
    10081 ttattgagtc aatttctgta tctttacttt taaaatacac attaggaaca attcttgtaa
    10141 gtattgctat cattgcacta cacctatgga ttgcaactgt aattccaaat caaatgattg
    10201 caattacatt tggcatgatt ggtagtttta taggtttgac atcaggtttg ttttatcaag
    10261 gtattcgtag attgtttata tggtcttatt acctagagtt atcaccttta agttatgctt
    10321 atgataatgt tttaggttca tctgtctata gaattgatat gaatttttct atcacgattt
    10381 tagttttctt aattggaatg ctaatttatt atattggaag aaatcattta ttaaaaaaag
    10441 aagtatagga gggatttaaa tgttaaggaa gtcagtcttg ggagaaattc taaagttaaa
    10501 aaattctttt atatggtatg tattattagc tttgccactt ataagtgtgt tgattggttc
    10561 tggtaatttt tatctaaatc aaggcatact caaaaaagaa tggtacagct tatggactca
    10621 agttagtctt ttttatgggg aatttttctt tcctatctta attgcaatct tttgtgcata
    10681 tatttgtagg cttgaacata tgaatcataa ttggaataat gtaatgacac tgccagttaa
    10741 tttaaaaaat atctttttat ctaaacttac agtagtttca atacttacag ctattacaca
    10801 agttttcttt gttatctttt atatagttgc aggaaatatg tttggttttt ctggtagtgt
    10861 acctaaagaa ttgactggtt ggattgctaa aggttggatt atttctattt caatagcttc
    10921 tatacaatta tacatgtcta taaagattag aagttttgca actcctattg gtattagtct
    10981 ttgcctttct ctatttggta tgggattaat agtagcaaaa cctgctgttg gtatattttt
    11041 cccttattct atgttaggta gtgctatggg ggttataaag caatctgctt taaatgttaa
    11101 tgacactata gttacaatat gtgttgcatt tattactact atagtatttt ctgaaattgc
    11161 caatagaaat tttagaaaga atgatgttat ttcttaatgg taaatgctag atatttttgg
    11221 gtttgaaatt tatattcttt ttagttgaaa tctatatcca ttttatttaa aaatatatat
    11281 cttttatttg aattttatat gttt
//
LOCUS       DBGJFF010000123        11186 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.125, whole genome shotgun sequence.
ACCESSION   DBGJFF010000123 DBGJFF010000000
VERSION     DBGJFF010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11186
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.125"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            <1..319
                     /locus_tag="ACOIAF_003084"
     CDS             <1..319
                     /locus_tag="ACOIAF_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGZ2670085.1"
                     /translation="FIFEQLLSNSLKYSSLGDNISIYCKRTKNYLQLRVEDTGCGIKK
                     EDLNRIFNKGFTGNNGRNNSKSTGLGLYLVKELCDKLGHKIDVDSEYNQYTKFTITFK
                     CDL"
     gene            431..1660
                     /locus_tag="ACOIAF_003085"
     CDS             431..1660
                     /locus_tag="ACOIAF_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HGZ2670086.1"
                     /translation="MSFLKKFKNKRFRNNSFKSNNFRNSSFGSSGFKSKNKGFKNNPF
                     KNKRFKIYAGIAVIILLILGVLAYVDSKNTELQANENFIETYTIPENEKIFINGMVVP
                     KQTKDFNISGDYELSDVNVTNGQKVNQGDLLFTAKNPTIIAEIDSLKSQLSQYKKQKI
                     SLSDIAENKDAIASINAQITALNSQIASLDKKAYDRVTAPFDGTVYLNDQTGNPDQPV
                     SFMTIQGLEFYMKGQASEQDLPKMKIDQMVNILVFSTDQKLTGRISFISDKPSTPNTE
                     MGAQQNTLSYYDINIAFDNQEGLVNGFHLQASLEVANSSFKIPASCVLKDNKHSYVFK
                     DLDGILKKQIVDVASQNDDFAVVRGGLEQGDIIIKHPTKEMKEGDPVQGDGVTAGSNN
                     GDTTPNKKVEEVTMDVN"
     gene            1668..2342
                     /locus_tag="ACOIAF_003086"
     CDS             1668..2342
                     /locus_tag="ACOIAF_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2670087.1"
                     /translation="MLIKLENIQKYYKVGKDELHVLKSLNLEIESGEFVMIMGKSGSG
                     KTTLLNILGFLDVFDEGRYIFDGTDVTNLSENERSVFRNINIGFVFQQFNLIETLNVY
                     QNVELPLIYNKALKKSNREEIVKDKLSSVGLLDKLKQKPLQLSGGQQQRVAIARCLAN
                     DPQIIFADEPTGALDSETSREIMELLTRLNKQGKTIIMVTHDQDLTKYATKVIRLKDG
                     VFTSEV"
     gene            2345..3577
                     /locus_tag="ACOIAF_003087"
     CDS             2345..3577
                     /locus_tag="ACOIAF_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2670088.1"
                     /translation="MSLLKNSLANLKGHKLRVFVALLWIIIGITSVILVSSIGNGFQK
                     EIKKSVNNVNPNKTTISFESADNTGLTDDMSIFLKPFNAKDLEELSFVEGVERIAPSR
                     DGFNLDSVYSSQASFDKKTTYVDVGPVKKDSKINLICGRDFSLDDEKRKVILLTLQST
                     SEIFENPEDALGHGININGTIFEIIGVLDDSQQNQAGGFFGGYQDMQFTTSLVPKKAF
                     DTLMSQNSYSNEIYQLDLVSSKGYNVNEVANNVIAKLYEMHPGINGSYTTPDPTEQTA
                     YLESINSNVNKYVSIITVVAMFVGGIGVMNIMYVSVMERQREIGIRRAIGAKPRSILF
                     QFLVEAVFITVCGGILGTIVGFAATNYVSKYIGFEAIPSLNSLFYAIVATILTGVVFG
                     LIPAFKASKLDPIKAIYK"
     gene            complement(3863..5128)
                     /locus_tag="ACOIAF_003088"
     CDS             complement(3863..5128)
                     /locus_tag="ACOIAF_003088"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HGZ2670089.1"
                     /translation="MILIKNGRVIDPKSQRDENIDLIIKENKIYKIGKFDESDEYEKI
                     IDASGNVVAPGLVDVHVHFRDPGFTYKEDIESGAKSAARGGFTTVICMANTNPIVDNE
                     DTFNYVKEKSKNACINVLQAAAITKGFEGKELVDMEALKKAGVPGFTDDGLPLMDSNL
                     IMEAMIKAKELDVPLSFHEEDPSLVGNPGVNAGKVASELGLKGASSVAEDVMVARDCM
                     LALKTGAKVDIQHISSGVSVDMVRFAKYLGATVVAEASPHHFTLTEEDVLEFGTNAKM
                     NPPLRSKWDRDKIIEGLNDGTIEIIATDHAPHSKEEKDREFIKAPSGIIGLETSLALG
                     ITNLVHKNHLSMMQLIEKMSINPAKLYNLNIGFIEEGAVADIVIFNPEEEWTVESFVS
                     KADNSPFKGKSLYGKVNYTICNGEIVYNA"
     gene            complement(5181..6329)
                     /locus_tag="ACOIAF_003089"
     CDS             complement(5181..6329)
                     /locus_tag="ACOIAF_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HGZ2670090.1"
                     /translation="MKSVSVRSCKTYNYEEVKLSIEKNLEDIGGIDKFVKKGSKVLIK
                     PNLLMKKKPEEATTTHPMVVKVVCEKLLELNCDITIADSPGGPYNQSSLKSIYKASGI
                     EEVANELGIKLNYDVSDVKIHSEKAHVLKYMDIIKPIVDSDYIINLCKLKTHIMATFT
                     GGTKNLYGCIAGLKKAEIHYRFPTEELFCENVLLDICDYIKPTLTIMDGIVGMEGDGP
                     SAGTTREIGVLLASQNPYAIDVVACKIISLLPNRVATIRGAIKRGYIQEDFSDIEILG
                     EDIKDLIIKNYKIPNVSKDLRLFSVKLPKFLNEPISKLITPKPVVRFQDCIKCGKCKE
                     ACPASTIEIGTKGAIIDLSKCIRCYCCHELCPKKAIDVKRNFVFKYVK"
     gene            complement(6429..6974)
                     /locus_tag="ACOIAF_003090"
     CDS             complement(6429..6974)
                     /locus_tag="ACOIAF_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HGZ2670091.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEMVWADE
                     HRIGVAQGVDAEIIEGLRANFTGECTEVGMYLAMSRQADREGYPEVAEAYKRIAFEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKRAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRHFGK"
     gene            complement(7197..8141)
                     /locus_tag="ACOIAF_003091"
     CDS             complement(7197..8141)
                     /locus_tag="ACOIAF_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HGZ2670092.1"
                     /translation="MQKNKKEGALLKKYFGEIGLIFIAIIWGSGFVATQFALDGGLTP
                     LQIITLRFFLAAIIMNLLFFKQIRANMGKKLLKAGGILGIFLFLAFTVQTIGLMYTTP
                     SKNAFITAANVVIVPFIGFILYRRKLDKIGIISSLVALIGIGILSLEADFSINFGDFL
                     TLICSFGFAFHIFFTSEFAKDNNPMALTAIQFTVAFLMSVVVQTFAGQLKMEAELSGY
                     MGTMYLAVFSTTIGFLFQTICQKRVDGTRTAIILSTEAVFGTIFSIIILKELITAKLV
                     IGSILIFVAIITAETKLSFLKSKKVKLKDSEESSLESI"
     gene            8527..9408
                     /locus_tag="ACOIAF_003092"
     CDS             8527..9408
                     /locus_tag="ACOIAF_003092"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HGZ2670093.1"
                     /translation="MVKKRKKKTVTLTILVVIVIGIFSMAWADMSRKKEMKETQERLK
                     AERLEKERIEKEKNEPIIGAKKEAVKEYGYDAKIVWDKLNKYDYSNNGEKIVFLTFDD
                     GPSTTNTPQVLDILKRHGVRGTFFIKGDSLERKGANEILKRTFDEGNAIAHHSYTHDY
                     KKLYPNRSLNLDTFVNELNKTDEAMKKVLGKNFSSNVVRCPGGYMSWKNMEPLGNYLK
                     EKNMASIDWNALNADAEGKKKNAQELFEHAKKSSEGKEMVVLLMHDTYGKQETVNALD
                     QIITYFKDNGYQFKILI"
     gene            complement(9469..10677)
                     /gene="tyrS"
                     /locus_tag="ACOIAF_003093"
     CDS             complement(9469..10677)
                     /gene="tyrS"
                     /locus_tag="ACOIAF_003093"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HGZ2670094.1"
                     /translation="MKSIDEQMRIIMKGVDDLIDEKELREKLIKSEKEGKPMIVKLGL
                     DPSAPDIHLGHTVVLRKMKQLQDLGHQIVIIIGDFTGKIGDPTGKSKARKALTTEQVL
                     ANAKTYEEQIFKVLDKEKTIVRFNSEWLAKLNFEDVIKLAATITVARMLEREDFKKRY
                     EGQMPISVHEFFYPLMQAYDSIALEADIELGGTDQRFNLLMGRSLQREFGMESQIVIM
                     MPLIEGLDGKEKMSKSLGNYIGIDEEAGIMYQKSMEIPDELIIKYYNLVTDVHPDEVN
                     KIESQLKEGSVNPRDIKMNLAREIVTLYHGEESAKEAEERFKSVFQKGQIPEDIQTIQ
                     VKEDGFDLIEVLVSNEIVKSKSEVRRLASQGGVKVNGEKVEDLSTIVKESELVVQIGK
                     KKFVKIELVK"
     misc_binding    complement(10750..11002)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         3825 a         1858 c         1442 g         4061 t
ORIGIN      
        1 cttcatattc gagcaattat tgagtaattc cctaaaatac tcctcattag gagataatat
       61 atctatctat tgcaaaagaa ccaaaaatta tttacaatta agagtggaag atactggttg
      121 tggtataaaa aaggaagatt taaatcgaat attcaataag ggatttacag gtaataatgg
      181 aagaaacaat tctaaatcta caggtcttgg gctttattta gtaaaagaac tttgtgataa
      241 gcttggtcat aaaattgatg tagattcaga gtacaaccaa tatacaaaat ttactattac
      301 attcaaatgt gatttatagt atcttattat tgatagttta ttttcattaa cttatattca
      361 acttcttata gtaattctat atgtacatcc cagtatataa ttaagtagtt tttataaaaa
      421 ggaggaagac atgtcttttc taaaaaaatt taaaaataaa agatttagaa ataacagttt
      481 taaaagtaac aactttagaa atagcagttt tggaagtagt ggcttcaagt ctaaaaataa
      541 aggttttaaa aacaatccct ttaaaaataa gagattcaaa atatatgcag gaatagcagt
      601 aatcatactt ttaattctag gagttcttgc ttatgttgat tcgaaaaaca ctgaattaca
      661 agctaatgaa aattttatcg aaacatatac tattcctgaa aatgaaaaaa tatttataaa
      721 tgggatggtt gttcctaagc aaactaagga cttcaatata tctggagatt atgagttaag
      781 tgatgttaat gttacaaatg gtcaaaaagt aaatcaagga gaccttcttt ttactgctaa
      841 aaatccaact ataatagcag aaatagatag tttaaaatca caacttagtc aatacaaaaa
      901 acaaaaaata tcactttctg atattgctga aaataaagat gctattgctt ctataaatgc
      961 acagataact gctttaaact ctcagatagc ttctctggat aaaaaagctt atgacagagt
     1021 tactgctcct tttgatggca cagtatactt gaatgaccaa actggaaatc cagaccaacc
     1081 agtttcattt atgactattc aaggtctaga gttttatatg aaaggacaag caagtgaaca
     1141 agatttacct aaaatgaaga ttgaccaaat ggtcaatata ctcgtttttt caactgacca
     1201 aaagcttact ggtagaataa gttttatatc tgataaacca tctactccaa atactgaaat
     1261 gggagctcag cagaatacac tatcttatta tgatataaac atagcatttg ataatcaaga
     1321 aggattagta aatggattcc atcttcaagc atcacttgaa gtagctaatt caagctttaa
     1381 aataccagca tcttgtgtac ttaaagacaa caaacactca tatgtattta aggatttaga
     1441 tggtatattg aaaaagcaaa ttgtagatgt agcaagtcaa aatgatgatt ttgctgtagt
     1501 tagaggcggt cttgaacaag gcgatataat aataaaacac cctactaaag aaatgaaaga
     1561 aggagaccca gttcaaggcg atggcgtaac tgctggttct aataatggag atactactcc
     1621 aaacaaaaaa gtagaagaag ttactatgga tgtgaattaa tttacatatg ttaattaaat
     1681 tagaaaatat tcaaaaatat tacaaagtag ggaaagatga attacatgtt ttaaaatcct
     1741 taaatttaga aattgaatct ggagaatttg tcatgataat gggtaagtct ggtagtggta
     1801 aaactacttt acttaatatc ttaggttttc tagatgtatt tgatgaagga agatatatat
     1861 ttgatggaac agatgtaact aatttgagtg aaaatgaacg ttctgttttt agaaatatta
     1921 atattggatt tgttttccaa caatttaatt taatagaaac tctaaatgta tatcaaaatg
     1981 tagagttgcc tctaatttac aataaagcac taaaaaaatc taatagagaa gaaattgtta
     2041 aagataaatt aagttctgtt gggcttttgg acaagcttaa gcagaaacca cttcaattat
     2101 ctggtggtca acaacagcgt gtagccattg cacgttgcct agcaaatgac cctcagataa
     2161 tctttgctga cgaaccgact ggggcattag atagtgaaac tagcagagaa attatggagc
     2221 ttttaacaag gctcaacaaa caaggtaaga ctataataat ggttacacat gatcaagatt
     2281 taactaagta tgcaactaag gttatacgac ttaaagatgg tgtgtttact tcggaggtgt
     2341 aatcatgagt ttattaaaaa attctttggc caatttaaaa ggtcacaagc ttcgtgtatt
     2401 tgtagcttta ctctggatta taataggaat aacttcagta atacttgtta gttctatagg
     2461 taatggattt caaaaagaaa ttaagaagtc tgtaaacaat gtaaacccta ataaaactac
     2521 tatttcgttt gaatcagccg ataacacagg tcttactgat gatatgagta tttttttaaa
     2581 accctttaat gcaaaagatt tagaagaatt atcatttgtt gaaggagtag aaagaatagc
     2641 tccttcaaga gatgggttta accttgattc agtttactct tcacaagcat cttttgataa
     2701 aaaaactacg tatgttgatg ttggaccagt aaaaaaagat tctaaaataa atttaatttg
     2761 tggtagagat ttttctttag atgatgaaaa aagaaaagta attttactta cattacaaag
     2821 tacaagtgaa atttttgaaa atccagaaga tgccttaggt catggtatca acataaatgg
     2881 aactatattt gaaataatag gggtattaga tgattctcag caaaaccaag ctggaggatt
     2941 ctttggaggt taccaagata tgcaatttac tacttcccta gtacctaaaa aggcttttga
     3001 cactttaatg agtcaaaatt cttattcaaa tgaaatttac cagttagact tagtttcttc
     3061 taaaggctat aatgtaaatg aagttgcaaa caatgttata gctaaattat atgagatgca
     3121 ccctggtatc aatggttctt atactacacc agacccaact gaacagacag cttatcttga
     3181 aagcattaat tccaatgtaa acaaatatgt atctataata actgtagttg caatgtttgt
     3241 tggtggtatt ggtgtaatga atataatgta tgtatctgtt atggaaagac aaagagaaat
     3301 tggtataaga cgtgctattg gagcaaaacc taggtctata ttatttcaat ttttagttga
     3361 agctgttttt ataacagtat gtggtggaat tttaggaact atagttggat ttgctgctac
     3421 taactatgtt tctaaatata taggttttga agctatacct agcttaaata gcttatttta
     3481 tgcaattgta gctactatat tgactggagt tgtatttggt ctgattccag cctttaaagc
     3541 ctctaaacta gaccctataa aggcaattta taagtagaaa ttgtataaag attttcgatt
     3601 tttagattag tttatacaaa acatacttaa atatataacg cactattata tatgatagat
     3661 taggaactta atctttccaa atataatagt gcgttctttc ataaaaagga ttctcttcct
     3721 tactatatct aaattaccaa gatgttatgt tctaattgtt caaactaata cttccaattt
     3781 tgattaatca atctcattct cctaatatac aatcttttac tttcaatcat tgtacactcc
     3841 gcctaaaaaa aataatctta aattaggcgt tatatacaat ctccccatta catattgtat
     3901 aattaacctt tccatataaa gatttacctt taaatggtga attatcagct ttagaaacaa
     3961 agctttcaac tgtccattct tcttctggat tgaatataac aatgtctgct acagcccctt
     4021 cttctataaa tccaatattt aaattgtaca actttgcagg atttatagac attttttcta
     4081 ttaactgcat catactaaga tgatttttat gcaccaaatt tgttattcca agagctaaag
     4141 aagtttcaag cccaataatt ccacttggag cttttataaa ctctctatct ttttcttctt
     4201 tactatgagg tgcatggtcc gttgctatta tttcaatagt accatcattt aatccctcta
     4261 taatcttatc tctgtcccat ttacttctta gaggtggatt catttttgca tttgttccaa
     4321 actccaatac atcttcctca gtcaatgtaa agtgatgtgg tgaagcttct gcaactacag
     4381 ttgcacctaa gtattttgca aatctaacca tatcaacaga tacacctgaa cttatatgtt
     4441 gtatatcaac tttagcacca gtctttagtg ccaacataca atctctggct accataacat
     4501 cttctgctac acttgatgca ccttttaatc ctagttctga agctaccttt cctgcattta
     4561 cacctggatt tccaactaaa gaagggtctt cttcatgaaa actaagtggt acatccaact
     4621 cttttgcctt aatcattgcc tccataatta gattgctatc cattaaaggt aatccatcat
     4681 cagtaaaccc tggtacacca gcttttttaa gagcttccat atcaactaac tctttacctt
     4741 caaaaccttt agttatagct gctgcttgta aaacatttat acatgcattt tttgactttt
     4801 ctttaacata attaaaagta tcttcattat ctacaatagg attagtattt gccatacata
     4861 taacagttgt aaatccacct ctagcagcag atttagctcc actttctata tcttctttat
     4921 atgtaaaccc agggtctcta aagtgtacat gtacatccac taaacctgga gcaactacat
     4981 taccactagc atctattatt ttttcatatt catcactttc atcaaacttg cctattttat
     5041 aaattttatt ttcttttata attaagtcaa tattctcatc tctttgactt ttagggtcta
     5101 ttactctacc atttttaatt aaaatcatat tatttctcct ttatattttt aatttatata
     5161 gactcatata atattataca ttattttaca tacttaaata caaaattacg tttgacatca
     5221 atagcctttt taggacaaag ttcatggcaa cagtaacatc ttatacattt acttaagtct
     5281 ataatagcac ccttagtccc tatctctata gtggatgcag gacaagcttc cttgcatttt
     5341 ccacatttta tgcaatcttg aaatcttacc acaggctttg gtgttataag ctttgaaata
     5401 ggttcattta aaaattttgg tagctttacg gaaaacaatc ttaaatcctt acttacattt
     5461 ggaatcttgt aattttttat aattaaatct tttatatctt cacctaaaat ttcaatatct
     5521 gaaaaatctt cttgtatata tcctctctta atagcacctc taattgtagc aactctattt
     5581 ggaagtaaac ttataatctt acatgcaaca acatctatag catatggatt ttgtgatgct
     5641 aataaaactc ctatttctcg agtagtacca gctgaaggtc catcaccttc catacctact
     5701 ataccatcca taattgtcaa tgttggtttt atataatcac aaatatctaa aagtacattt
     5761 tcgcaaaaca actcttctgt aggaaatcta taatgtatct ctgctttttt caatccagct
     5821 atgcatccat ataagttttt tgtaccacca gtaaaagttg ccattatatg agtctttaat
     5881 ttacaaagat ttattatata atctgaatct actattggtt taataatatc catatatttt
     5941 agaacatgtg ctttttcact atgtatctta acatctgaaa catcataatt taacttaatg
     6001 cccagttcat ttgctacttc ttcaattcca gatgccttat aaatgctctt aagagaactt
     6061 tgattatatg gaccaccagg gctatctgct attgtaatat cacaatttaa ctctaaaagt
     6121 ttttcacata caacttttac aaccatagga tgtgtagttg tagcttcttc aggctttttt
     6181 ttcatcaata aatttggttt tataagtact ttgcttcctt ttttaacaaa cttatctata
     6241 ccaccaatat cctctaaatt tttttcaata gataacttta cttcttcata attgtaagtt
     6301 ttacaactcc taacagatac cgacttcata ttatctccat ccttctatca caacagttta
     6361 tttgtataat ttaaaaaagg agaaaccaaa gtttctcctt ttaatctatg ttttaattgt
     6421 tttaattatt attttccaaa atgtctgttt aataatccta agaatgcttt accatgtcta
     6481 gcttcgtctt tacacatttc atgtactgta tcatgtatag catctaatcc taattcttta
     6541 gctctcttag ctaattttaa ttttccatca gttgcaccat actcagcatc aactctaact
     6601 cttaagtttt cttttgtatc tgcaactaca acttctccaa gaagttctgc aaatttagca
     6661 gcatgttcag cctcttcaaa agctattctc ttatacgctt cagctacttc tggataacct
     6721 tctctatcag cttgtctact cattgctaaa tacattccta cttctgtaca ctcaccagta
     6781 aagttagctc ttaatccttc gattatttct gcatctacac cttgagctac tcctattcta
     6841 tgttcatcag cccaaaccat ttcgcctttc atttcttcaa atttatcagc tccaacttta
     6901 catactggac attgtgctgg tgcagcatct ccttcatgta tatatccaca tactgtacaa
     6961 acaaattttt tcataattaa tttcctccca attttatata attaaatttt tatttttaag
     7021 tttaataact tgctacaaga atatatatac cccagacttt atagcttaaa cactaaaaat
     7081 aaaatttatt tcctaattaa ttttatcact ttgaaacacc agtatcaacc aagttttgat
     7141 gtcaaatttc tacataatac ataatttatt tgattaatta ggaaatagtt gtcatattaa
     7201 atactttcta atgaagactc ctctgaatct ttaagtttca cttttttgct ctttaaaaaa
     7261 gataatttag tttctgcagt tatgattgca acaaatatca agatactacc aataaccaat
     7321 tttgctgtaa taagctcctt taaaattatt atagaaaata ttgtaccaaa tacagcctct
     7381 gtggataata ttatagctgt tctagttcca tcaactcttt tttgacatat agtctgaaat
     7441 aaaaatccaa ttgtagtact aaatacagct agatacatag ttcccatgta accagataat
     7501 tcagcttcca tctttaattg acctgcaaat gtttgaacta caacagacat taaaaacgct
     7561 actgtaaatt gaatagctgt caatgccatt gggttgttat cttttgcaaa ctcacttgta
     7621 aagaatatat gaaatgcaaa tccgaatgaa catataagtg ttagaaaatc tccaaaatta
     7681 atagagaaat cagcttctaa agaaagtatc ccaattccta taagagctac caaactactt
     7741 attattccta ttttatcaag ttttcttcta taaagtataa atcctatgaa tggaactatt
     7801 acaacattag ctgctgttat aaacgcattt ttagatggtg tagtgtacat aagtccaata
     7861 gtttgtactg tgaatgctag aaataaaaat attcctaaaa tccctcctgc ttttaataat
     7921 tttttaccca tattggcacg aatctgttta aaaaacagta aattcataat tatagctgcc
     7981 aagaaaaatc ttaaagttat gatttgtaga ggtgtaagcc caccgtctaa tgcaaattgg
     8041 gttgctacaa agccacttcc ccatataatt gctataaata ttagtcctat ttctccaaag
     8101 tattttttca ataaagcccc ctccttttta tttttttgca aaattataaa ccccctaaac
     8161 agcaagagag tttttctata agcattacta taaattaata tagtaaattt tttcagtatt
     8221 ttaaatctaa atttacatac attttgagct caaatttata tccactattt aacagtaata
     8281 tattaatcat tttttgtaaa caccttttta ataaattttt atatttttta tattttttta
     8341 cctatactaa atttttttat agatattttt aatatatatg atactttaat attatgcaaa
     8401 taatgtcata taagttacac tttagttatc ttagttacaa ctttgtcggt ttactttttc
     8461 tactttcttc gatataatta taatatattt ttgatacaat tcgacaaaag ggggaatgct
     8521 gtaattatgg taaaaaaaag aaaaaagaaa actgttacac ttactatact tgttgtaata
     8581 gttattggta ttttttctat ggcatgggcg gacatgtcta gaaaaaaaga aatgaaagaa
     8641 actcaagaaa gacttaaagc tgaaagatta gaaaaagaaa gaattgaaaa agaaaaaaat
     8701 gaacctatta ttggtgcaaa aaaagaagct gtaaaagaat atggatatga cgcaaaaata
     8761 gtttgggaca aacttaacaa atacgactat tcaaacaatg gagaaaagat agtctttctt
     8821 acatttgatg atggaccatc tactactaat actccacaag ttctagatat cttgaaaaga
     8881 catggtgtta gaggaacttt ctttattaag ggtgattctc ttgaaagaaa aggagctaat
     8941 gaaattttaa aaagaacatt tgacgaagga aatgcaatag cccatcactc ttatactcat
     9001 gactataaaa aactatatcc taacagaagc ctaaatctcg atacattcgt aaatgaactt
     9061 aacaaaactg atgaagctat gaaaaaagtc ttaggaaaga atttttcctc aaatgtagtt
     9121 agatgcccag gcgggtacat gtcatggaaa aatatggaac ctctaggaaa ctacttaaaa
     9181 gagaaaaaca tggcatcaat tgattggaac gctttaaatg ctgatgcaga aggtaaaaag
     9241 aaaaatgcac aagaattatt tgaacatgca aagaaatctt ctgaaggcaa agaaatggtt
     9301 gttcttttaa tgcatgatac ttatggaaaa caagaaacag taaatgcatt ggaccaaatt
     9361 ataacatatt ttaaagacaa tggttatcaa tttaaaattt taatataata aaaaagcaag
     9421 tttattgaat gtacatttta tactcaatag acttgctttt actttactct attttactag
     9481 ctcgattttt acaaattttt tctttcctat ttgaacaact aattcactct cttttacaat
     9541 agtgcttaaa tcctctactt tttcaccatt tacctttact ccaccttgag aagctaatct
     9601 tctaacttca ctcttactct taacaatctc attagaaact agtacttcga ttaagtcaaa
     9661 tccatcttcc ttaacttgaa ttgtttgtat atcttcaggt atttgacctt tttggaatac
     9721 agatttaaat ctttcttcag cttctttagc agattcttct ccatggtata atgttactat
     9781 ttctcttgct aagttcatct ttatatctct tgggtttact gaaccctctt tcaactgaga
     9841 ttctatttta ttaacttcat caggatgaac atctgtcact aaattgtagt atttaattat
     9901 aagttcatca ggaatttcca tagatttttg gtacattatt cccgcttctt catctattcc
     9961 aatatagtta cctaaactct tactcatttt ttctttacca tctaatcctt ctattaaagg
    10021 catcattatt actatttgag actccatacc aaattctctt tgtaatgatc ttcccattag
    10081 taaattgaat ctttggtcag ttccaccaag ttctatatca gcttctaatg ctatagagtc
    10141 atatgcttgc attaatgggt agaagaactc atgtactgat attggcattt gtccttcata
    10201 tctcttttta aaatcttctc tttctaacat tctcgcaaca gtaatagttg ctgctaattt
    10261 tattacatct tcaaaattta acttagctaa ccactcgcta ttaaatctta caatagtttt
    10321 ttctttatct aaaactttga aaatttgttc ttcataagtt tttgcatttg caagaacttg
    10381 ctctgttgtt aaagcttttc tagctttaga ttttccagtt gggtctccaa tttttcctgt
    10441 aaaatctcca attattatta ctatttgatg acctaaatct tgtaattgtt tcatctttct
    10501 aagaactact gtatgcccta aatgtatatc tggagcactt gggtctagac ccaattttac
    10561 tatcataggt tttccttctt tttctgattt aataagcttt tcccttaact ctttctcatc
    10621 tattaaatca tctacacctt tcataattat tcgcatttgc tcgtctatcg atttcatttt
    10681 tcattcctcc taaaatattt atataatcta taatttttat ttttataata aaaaaagctt
    10741 tcatcctctt aaaaggacga aagcattgct tacgtgttac caccttagtt catcaatatc
    10801 tcacgatatt aatctcttag agtacaatta catatactcg tgtactataa cgtgtactaa
    10861 tccgatatct cttaatggtt gttatctttc agagatagtg ctctgagatg tattcaaact
    10921 aaatcaattt attccatttc accaaactgg aactctctgt taaataaaat ctagccctac
    10981 ttcttctctt catagcattt atatattcag attatttcat caattacaag aaatttttct
    11041 taacttactc ataatatatt attattttta tattttgtca acctttttta ttattatata
    11101 catgtttttt tacattatac tactttttta tattttttac caatacccaa aatttatttt
    11161 tcaatcaaaa gatttatttg tttatt
//
LOCUS       DBGJFF010000124        11087 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.126, whole genome shotgun sequence.
ACCESSION   DBGJFF010000124 DBGJFF010000000
VERSION     DBGJFF010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11087
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.126"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(38..670)
                     /locus_tag="ACOIAF_003094"
     CDS             complement(38..670)
                     /locus_tag="ACOIAF_003094"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HGZ2670095.1"
                     /translation="MTIPSSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVPPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIKHIQSGNIDKLV"
     gene            complement(763..1095)
                     /locus_tag="ACOIAF_003095"
                     /pseudo
     CDS             complement(763..1095)
                     /locus_tag="ACOIAF_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009271880.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase DNA-binding domain-containing protein"
     gene            complement(1173..1376)
                     /locus_tag="ACOIAF_003096"
     CDS             complement(1173..1376)
                     /locus_tag="ACOIAF_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HGZ2670096.1"
                     /translation="MNNTDVPIWEKYTLTIEEASKYFRIGEKKLRKLADENLDSGWVI
                     MNSNRIQIKRKQFEKIVDTLDVI"
     gene            complement(1766..1996)
                     /locus_tag="ACOIAF_003097"
     CDS             complement(1766..1996)
                     /locus_tag="ACOIAF_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGZ2670097.1"
                     /translation="MKPTKEVLPFTIILLAVHGDVNAINGILKHFEHYIIRLSQKTLF
                     DEYGNPYIHVEEETKRMLGTKLITAILRFNLS"
     gene            complement(1998..2351)
                     /locus_tag="ACOIAF_003098"
     CDS             complement(1998..2351)
                     /locus_tag="ACOIAF_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HGZ2670098.1"
                     /translation="MRNYARDIYDENKRRNDYLVSLESLSLAELSKLSILDDYDSNYI
                     CMVSYDYNIRIEDVLMAQAIGKLTKRKQDIILLSFFLNMTNADIATLMDLAENTIHYH
                     KTNALKELKELMEEH"
     gene            complement(2765..3214)
                     /locus_tag="ACOIAF_003099"
     CDS             complement(2765..3214)
                     /locus_tag="ACOIAF_003099"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGZ2670099.1"
                     /translation="MELIEWNYAYITELVEFANDKEIANNLRDNFPHPYREQNARDFI
                     SFCLATPDVKQLSYAIFYQERAIGSISLTFEEDTSINKNAELGYWLARNYWGKGIATN
                     AIKEICKIAFEKYNLNKIHANVYSYNRGSSKVLEKCGFKIEEYCHNS"
     gene            complement(3228..4154)
                     /locus_tag="ACOIAF_003100"
     CDS             complement(3228..4154)
                     /locus_tag="ACOIAF_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670100.1"
                     /translation="MSDILSRITNKIGDKNIVDKLSALSKSDLNSLLLEVFDRQANTL
                     TATDILKSYQLNRFTIPSGIDPEEIHALESNLLRKAFNMDIKTIMLSPSAPLGSCSVF
                     GSVDQYNVVSALRGTETLADPSNMLAIIIADKLKRKEENNTIPIHMATTARVIRAQNF
                     VGKGLYSHFGLYCMVSSGIDTGSYTCEKMLLEKHLNYYKDILSEIENVRFSIILRKRN
                     GYKDNDGFFDRMVETIKLIFPNIELSIHNDDVDNNYYKGINFKISVKQGNETVEIADG
                     GFVDWTYQMLGNKKERCLISGIGLERFLVAVS"
     gene            complement(4279..5061)
                     /locus_tag="ACOIAF_003101"
     CDS             complement(4279..5061)
                     /locus_tag="ACOIAF_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HGZ2670101.1"
                     /translation="MTKTQQKMIQNVLEFSKRDSRIIGLAISGSYITKSLDEYSDLDF
                     LIVADDKSYEEVIEERLLIVEQFGTLVSAFTGEHVGEPRLIISLYDSPVLHVDFKFTT
                     LYGLLDRVEDSAILYEENNCITEIYSKKEAYFPTPDLQWIEDRFWIWVHYACTKIGRR
                     ELFETIDFLSFLRQSVLAPLIQLLLGKLPRGVRKIELDAPQYIEALEKTVANHEYKSC
                     VNALQATINLYLELREELSQSPIVRKVEAERVALQYFQDLIK"
     gene            complement(5217..5837)
                     /locus_tag="ACOIAF_003102"
     CDS             complement(5217..5837)
                     /locus_tag="ACOIAF_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HGZ2670102.1"
                     /translation="MHLIDFMNEDIKKKLVKKTYKKKETILFAEQENEYVILMIDGIA
                     EAFILNVKGNISTIHLYRSGSFFGEMEQFNDGKKPVEIVAFTDCNVYKLHRDDFLNWL
                     RGDFEATKFLIREISSKLVVNAELIEELSSLSVKERLLRCISLHYYRSELNLLTKKQL
                     ASEVNAPIRSINRAINECVQEHLIAYENKRFSVINQREVLKNLPTT"
     gene            complement(5964..6632)
                     /locus_tag="ACOIAF_003103"
                     /pseudo
     CDS             complement(5964..6632)
                     /locus_tag="ACOIAF_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            6697..7539
                     /locus_tag="ACOIAF_003104"
                     /pseudo
     CDS             6697..7539
                     /locus_tag="ACOIAF_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220015.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            8211..8978
                     /gene="aroD"
                     /locus_tag="ACOIAF_003105"
     CDS             8211..8978
                     /gene="aroD"
                     /locus_tag="ACOIAF_003105"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HGZ2670103.1"
                     /translation="MKRKVQVKNITIGEGRPKICVPIIGKNKKDIIKEAKELKDACLD
                     IIEWRVDFFENVENIKEVKEVLYELRSYIHDIPLLFTFRSVVEGGEKLISRDYYTTLN
                     KEISNTGLVDLIDVELFMGDEVIDEVVNFAHKKEVKVIISNHDFNKTPKKEEIVSRLC
                     RMQELGADLPKIAVMPQNEKDVLVLLEATNEMFKIYADRPIITMSMSGMGVISRLCGE
                     IFGSALTFGAAKSVSAPGQISFKELNSVLNLLHKSIN"
     gene            complement(9306..9983)
                     /locus_tag="ACOIAF_003106"
     CDS             complement(9306..9983)
                     /locus_tag="ACOIAF_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670104.1"
                     /translation="MIVIEGSDKFKIAKEYIDVEYTLFSKVTFRYEKLKFKDNAELEK
                     IKMFKYKNGYIPNKLNLSFGYGFSSYKKQIIRETVDTLRLTEIFSSENIEDIKFIKDG
                     TKKLEISIEKVVKFKRRKKRNYVCCYCPDMYRDIKLDKESINKIYNRKIKIEREVNIF
                     EDEDVIINKKVLKFPKSWTKNMQKYWLSENKYPIHSTVIDDDRYKCCNVQYTKNRVII
                     LYYIYNH"
     gene            complement(10133..10450)
                     /locus_tag="ACOIAF_003107"
     CDS             complement(10133..10450)
                     /locus_tag="ACOIAF_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGZ2670105.1"
                     /translation="MNYIGKRLKEERKKANLTSKEFANMVGVSPWYITQIESGKKNPS
                     LKTFIKFVNILNISADVLIKDITSTGKTYLENDINEELKDLNSRELNLISQIIDIIKK
                     NKI"
     gene            complement(10472..10810)
                     /locus_tag="ACOIAF_003108"
     CDS             complement(10472..10810)
                     /locus_tag="ACOIAF_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGZ2670106.1"
                     /translation="MANIGKIIGDRIKVYRTKLGYSQEFLAEKAGLHPTYIGQIERAE
                     TNTSINIIMKIATALDIPLELLFANIITTEGVDNSVPLECYEMINKLTEKEQIAMLEL
                     IKCIIKYKEI"
BASE COUNT         3688 a         1737 c         1570 g         4092 t
ORIGIN      
        1 attaaagtaa agtttaatat aaataaatta taaaatatta aactaattta tcaatatttc
       61 ctgattgaat atgcttaatg tttgattgaa tttttttata tggccaatcc caccatttaa
      121 tttctaataa ttttgaaatt gtatcattat taaatctctt ttttataatt tttgcaggta
      181 ctcctccaac aatagaatac ggtgggacgt cattagtaac tactgctctt gttccaataa
      241 ttgcaccatc accaatattt acacctgaca taattataga atcataacct atccacacat
      301 catttccaat tataatgtta cccttattgt cccaagcgtc cgttatatgg ttgatatcga
      361 gtttccattc ttcataaaag atgggaaaag tatatgtgga ttttgatttc attgagtgat
      421 ttccactagt cattagaaat tttgcattac atgctattga gcaaaactta ccaattatta
      481 atttatcatt gtttataggg taatgataca atacattgtt tttctcaaaa tctcttgggt
      541 cattataaaa gtcattatat attgtgtaat ctccaacttc tatattttcc cttgttatta
      601 catttttaag atatacagtt tgataatcat tacttcttgg atatacctta tttgaacttg
      661 gaatagtcat atttaaatct ctcctttaat atatattatg tttttgacta ttccactact
      721 acaatacatc tcttcacaga atcactcctt gttaagaatc ttcataagat aatggcgtcc
      781 ttttttagtg ttaagtcgga tattctttct tagtacattc tttttagcgt ataactgaca
      841 aactgtcatt ttcttaccaa tagtgtcaat gctatcatca aggtcatttt gtattgcttt
      901 ttccttatcc cttaatgata tatcatcacg ttttcccttt gggggtttat cagtaggtac
      961 aagtttccaa gagtaaacaa attgtggttt accaaatgaa tatacatact tatatgcgta
     1021 tcttccatct tttctctggc tctctccagt tcttaaaatt ctatctttat tatctcgtct
     1081 tttttcagtt gacatgataa gaagctcctc tcaataaccg aattgagctt gatacgtcat
     1141 atgaatatta taacatataa ggctcaattt cattagataa cgtcaagtgt atcaacgatt
     1201 ttctcaaatt gttttctctt tatttgaatg cgattgctat tcataatgac ccagccagaa
     1261 tcaaggtttt catcagcaag tttacgtaat tttttctctc caatacgaaa gtattttgac
     1321 gcttcttcaa tcgtcaaggt atatttttcc caaataggta catcagtatt gttcatagaa
     1381 tgtatcactc ctttctatgt tcgtagttca gaagtagata gatggctttg gtaagctcca
     1441 ttggtatttc aaccaatccc attcttatgg gcagaaccgt gtcatacagg tgtatcatta
     1501 tactgtggta caggttacgg caaaacaacc gttccaaagg ttattcacaa atcactatat
     1561 agtagctctc ttgattaagg tcatggcgta tagtatttgt tggctatctg tatcacaaac
     1621 gtatctatct actttattca gttgtcaaag aacagtcggc attgatttgc ctataaaaaa
     1681 tcacaatagc ttgtgggctt ttatagacaa ataattagtg attctccatg taccaaatag
     1741 cttgatacat ggagagaggg cgagattaag acaaattaaa tctcaaaata gcagtgatta
     1801 gttttgttcc tagcattcgc tttgtttctt cttctacatg aatataggga tttccgtatt
     1861 catcaaataa agttttctgt gaaagacgaa tgatatagtg ttcaaaatgt ttaagtattc
     1921 cgtttattgc atttacatcg ccatgtacag caagtaaaat aattgtaaaa ggtaatactt
     1981 ctttagtagg tttcatttca atgttcctcc atgagttctt tcaactcttt tagtgcattt
     2041 gttttgtgat aatgtatggt attttctgct aaatccatca atgttgctat gtcagcattt
     2101 gtcatgttga gaaagaatga aagtagaatt atatcttgtt ttctttttgt aagttttccg
     2161 attgcttgtg ccattaatac atcttcaatt cgtatgttgt agtcgtaaga aaccatacag
     2221 atgtagttag aatcataatc atctaaaata ctcaatttac ttaattctgc aagtgacaaa
     2281 gattcgagag ataccaaata atcatttctg cgtttatttt cgtcataaat atctctagca
     2341 tagttacgca aaactgtttt acagaaactg tcgaattgat gttcaattgt cttttcatca
     2401 accataagat ttcaccctct ttccatcgca aggagttaaa taaccttgct tactcctatc
     2461 acggtgggag ggaattaaat cttcgataaa tttcaaaaaa aagagaaaaa aatacacact
     2521 ctgcctatag ggcaaaaatg tgtattcttt aaaacgtatt taatttatat tggttgtaag
     2581 ccaccaccat attgatgtat ggaaatatgc attggctagc ttttcataaa aatacaaaga
     2641 tgcataagaa attaatagag gggaaaaact tattctgtta ttaaaaagta ggttaattct
     2701 aataaatgct attgcggtcg aattaaacta tatacatggc aatcaatcat gtttctattc
     2761 tttattatga attgtgacag tattcttcaa tcttaaaacc acatttttcc aatactttac
     2821 ttgaccctct attgtaggaa tatacattag catgaatttt gttaaggttg tatttttcaa
     2881 aggcaatttt gcatatttct tttattgcat ttgttgctat ccctttgccc caatagttcc
     2941 tagcaagcca gtaacctaat tcagcatttt tattaataga ggtatcttct tcaaatgtta
     3001 ggctgatact tccaatagcc ctttcttgat aaaaaatagc atagctaagt tgtttaacat
     3061 ctggagttgc caaacaaaaa ctaataaaat ctctagcgtt ttgctcccta taagggtggg
     3121 ggaagttgtc acgaagattg tttgcaattt ctttatcatt cgcaaactca actaactcgg
     3181 taatataagc ataattccac tctataagtt ccatttttct tctcctttta tgaaacagct
     3241 acaagaaatc tttctaagcc aataccactt atcaaacagc gttctttttt atttccaagc
     3301 atttgataag tccagtcaac aaatccaccg tcagctattt caacggtttc atttccttgt
     3361 ttgaccgata ttttgaaatt gataccttta taataattat tatcaacatc atcattatga
     3421 attgataact ctatattagg aaaaataagt ttgatagttt caaccattct gtcgaaaaat
     3481 ccgtcattat ctttgtatcc gtttcttttg cgtagaataa tagagaaacg aacattttct
     3541 atttcactta atatatcttt ataatagttt aagtgctttt ctaacaacat tttttcacaa
     3601 gtgtaagacc ctgtatctat accagatgaa accatacaat ataagccaaa atgtgaatat
     3661 aaacctttac ctacaaaatt ttgtgctctt attaccctag cagtagtagc catgtggata
     3721 ggtatggtat tattttcttc ttttcttttt aatttatcag caataattat tgccaacata
     3781 tttgaggggt ctgcaagtgt ttctgtccct cgtagagcac ttactacgtt atattgatct
     3841 acggagccaa aaacgctaca gctacctaat ggggcagagg gagaaagcat aatcgtcttg
     3901 atatccatat tgaatgcttt tctcaataaa tttgattcca aagcatgaat ttcttctgga
     3961 tctataccgc tagggatagt aaatctattt agttggtatg actttagaat atcagttgca
     4021 gttaaagtat ttgcttgtct atcaaacact tctaatagaa gtgagttcaa atctgatttt
     4081 gatagagctg ataacttatc aactatattc ttatccccaa ttttatttgt aattctactt
     4141 aaaatatcac tcatagttat cctccatact gttactttca aaataattat agaattgtta
     4201 caacaactaa tctaggtcat ttgaccaaaa atgaaaaaac actgaatatt tactattcaa
     4261 tattttttta ctttgggttt atttgattaa atcttgaaaa tactgtaatg caaccctttc
     4321 agcttcaacc tttcggacaa taggactttg tgataattcc tctcttaact caagatacag
     4381 gttgatagta gcctgcaatg cattaacaca gcttttatat tcatggttag caaccgtctt
     4441 ttctaatgct tcaatatatt ggggtgcgtc caattcaatt tttctaactc cacgaggtaa
     4501 tttacctaag agaagttgta ttaatggggc aagtactgat tgtcttaaaa aagataaaaa
     4561 atcaattgtt tcaaatagtt ctctacgtcc aatcttagtg caagcgtaat gaacccaaat
     4621 ccaaaatcta tcttcaatcc actgtaagtc tggagttggg aagtatgctt cttttttaga
     4681 ataaatttct gttatgcagt tgttttcttc atagagaata gctgaatctt caaccctatc
     4741 tagtaaacca tataaggttg taaacttaaa atcaacatga agaacagggg aatcatagag
     4801 ggaaattatc aatcgaggtt ctccaacatg ttcgccagta aaagcagaaa ctaatgttcc
     4861 aaactgttcc acaataagta atctctcttc gataacttct tcataggatt tatcatctgc
     4921 tacaatcaga aaatccaaat cagaatattc atctaacgat tttgtgatat aagaaccgct
     4981 aattgcaagt cctattatcc tactatctct ttttgaaaat tctaatacgt tttgtatcat
     5041 tttctgttgt gttttagtca taatataaca cctctcactg tcttttatta gctatcaatc
     5101 caacataatt atgcatagga gtattatata atgaagaaat aaaaaaaaat aggtcacttg
     5161 acccgaaata aaaaagcatt gaataattat tattcaatac ttttttgaaa ttatacttat
     5221 gttgtaggaa gattctttaa tacctctcgc tgattaatta cactaaatct tttgttttca
     5281 taagcaatta gatgttcttg tacacattca tttatagcac gattaatgct tctaattgga
     5341 gcgtttactt cgctagcaag ctgttttttg gtaagcagat ttaattcact tctataatag
     5401 tgtaaggata tacacctcag aagtcgttct ttaacactta aagatgataa ttcttcaatt
     5461 aattcagcat ttacaactaa tttactagat atttctcgaa tcagaaattt tgtagcttca
     5521 aaatctcccc ttaaccagtt taaaaaatca tctctatgaa gtttatatac attacaatct
     5581 gtgaaagcga caatttcaac gggttttttc ccatcattaa attgttccat ttctccaaaa
     5641 aaactaccac ttctataaag atgaatcgtt gagatgtttc ctttcacatt taagatgaat
     5701 gcttctgcaa taccgtcaat cataagaatt acatattcat tttcttgctc agcaaaaaga
     5761 atagtctctt tctttttata cgttttttta acaagcttct ttttaatatc ttcattcata
     5821 aaatcaatta aatgcatatt catccctcct taccaaatct ttgattaaat tatatcatct
     5881 ttcaattagt taggtaagat agtaaaaata tatttacata gaaactattc cattattatt
     5941 ttccagttgt catttttctg caaaaactta tcactgtcac gactggagat accagtaaga
     6001 aatgttagga tatcaatcgc taaactttca gaatcctttt tacggctcaa aatgacgtaa
     6061 tggtcaagca atcctttgtt ttcatcatca ctggttaaat tgacaatgtt tatctcatgg
     6121 gctatttctg gtagcgtttc tttccatttg ccgcgttttg ctttagggtc aactatgacc
     6181 gcctatccgc cgaatagtac cgcatagtaa acaagcatat tgttactgaa taatttacca
     6241 ccacccaatg aaccgataaa cgcagacgct aacgcattgg taatagaacc ctttacccct
     6301 tgactagcaa gggcaggttt tagatagaca ttctttccag tgtccagatt gtagctaaca
     6361 tagatatcct catgttcgcc aagggtttgt gtcgctccaa aaccaagtcc agccaaaaaa
     6421 tccgaagtga catattgaat ataatcattg atgtaacgtt tgctgacagg aataaattcg
     6481 ccatgcaatc ccaacatatc cccaaaggga tgcacgagct tgattgataa gtcgtcgtaa
     6541 aagtctttga cttcatcaca atgccttttt agctcgtcaa gatcctttgt agatacacgg
     6601 acaacatagc taagtttata catagattct ttacagttaa aagtctgttt ttaaaaaaat
     6661 gtataaatta taaatcaaaa tatagatact atcaatgacc cactaggtgc aaaaagcatt
     6721 gatatggtta aattatcaga cgctaaaagc tgggctatcc gaatgagtga agatgggtgt
     6781 gcttataaat ccattggcaa ttataagcgc tcactgaaag cgtcgttttt catggcaatt
     6841 caagacgact gtattagaaa gaatccattt aattttcagt taagtgatgt tttggaagat
     6901 acaacagaac caaaaatcgc tctaacacca gaacaggaaa tgggcttatt atcctttatg
     6961 gagagcgata agacttattg taaatattat gatgatgttg ttatcctctt agaaacagga
     7021 ctacgcattt ctgaattatg cggtttgact ttagaattgg atatgaaaaa taaagccatt
     7081 atggtagacc atcaattgtt gagtgatact aagattggct attatgtaga agttccaaag
     7141 accacacagg gtaagcgaga aattccaatg actgaaagag cttatcaatc atttgaacgt
     7201 atcgttgaac ggcgtaagaa cgctaaagca gaaccaatca ttgttgatgg ttaccagaac
     7261 ttcctatttc ttaaacaaga tggattacca aaacaagcaa ctcattatca aagcatgtta
     7321 agagcgttag tcaagaagta taacaagacc catgaggaca agctaccaaa gattacgcca
     7381 tatatcttta tgcatacact ctgtacaaat atggctaaca aaggaatgag tcctaatata
     7441 ttgcaatata ttatggggca taaaaacatt actatgacgc ttggctatta tgcacatggt
     7501 tcatttagtt ctgcgagagc tgaaatggaa cggttataag cctagtagta tgactagagt
     7561 ttactactta tttactactt ttgatggcat tcttatacgg ttaaatgcca acttatgcaa
     7621 agtatcttcc aaaaagaaaa tgtcgatact ccttgtagat acaggatata tcgacattta
     7681 ccaacttatg aaaagataat caaatttaat ataagttttt caaataaaaa gatactaaat
     7741 tctttttcga gaatctatat aacattactt aagcttggtt ggggtataaa caattgagta
     7801 gctacttttg tatggtgttc aactttagtc aaaactctta aataatgcaa atgatttaga
     7861 ttcattaata ttcaatctct tcataagtag tgaaattcga gttatttctg cataattata
     7921 ctatgattta tagattaatt ctattagcat ccagaataat attgtttata tgaataaaat
     7981 ttaaagaata ttatgaaaat tataggatgt taaaataaat ttaggtaaaa aagaagtgtt
     8041 tgtatattat agagtacctt ttctaaaata tgtgggattg ttttaagaat tagaataaaa
     8101 attattttta aattagtatt tttataatgt atagtatttt taatgtataa ttaaattgtg
     8161 cagaaaatag aatttaaagt cctatagaat ttctgattat ggaggagaat atgaaaagaa
     8221 aagttcaagt aaaaaatatt actattggag aaggtagacc taaaatttgt gtaccaatta
     8281 ttgggaaaaa taaaaaagat attataaaag aggctaaaga gttaaaagat gcatgtttgg
     8341 atattataga gtggcgagtt gatttttttg agaatgtaga aaatattaaa gaagttaagg
     8401 aagttctcta tgaattaaga agttatatac atgatatccc tttattattc acatttagaa
     8461 gtgtagtaga agggggagaa aaattaatat caagagatta ttatactact ttgaataaag
     8521 aaatttctaa tacaggttta gtagatttaa tagatgtaga gttatttatg ggagatgagg
     8581 tcatagatga agtagttaat tttgctcata aaaaagaagt gaaagtaatt atatctaatc
     8641 atgattttaa taaaactcca aaaaaagaag agattgtatc tcgtttatgt agaatgcagg
     8701 aattgggcgc agatttaccc aaaatagctg ttatgccgca aaatgaaaaa gatgtgttag
     8761 tgctacttga agctacaaat gaaatgttta aaatttatgc agatagacca ataataacaa
     8821 tgtcaatgtc aggaatgggt gttataagta gattgtgtgg tgaaatattt ggttctgcat
     8881 tgacttttgg agctgcaaaa agtgtttcag caccagggca aatatcattt aaagagttaa
     8941 attcagtact taatctatta cataagagta ttaattaaat taattatata gaaatattag
     9001 acggtatttt tgaagatata aattaaatat aaaaaacttt tcagatagta aaatttaaat
     9061 tgggcaatta tatctgaaaa gcaactattt agtgtgtatt ttgaaagtga tatagaaaat
     9121 cttataaatc aactatggca tatttaaaat atgccattaa aacctactag acattaggtt
     9181 atagatggta tactagcatt tctattattt atagatagta agttttcata ataactgttt
     9241 tatctaaagt agaagatata tttgtattat gtatatttgt cattattttt ttgtaattat
     9301 gtatattaat ggttatatat gtaatataat attatcactc tattttttgt atattgtaca
     9361 ttacaacatt tatatctatc atcatcaatt acagtagaat gtatgggata cttattttca
     9421 cttaaccaat atttttgcat attctttgtc caagactttg gaaacttcaa tactttcttg
     9481 tttattataa catcctcatc ttcaaaaata ttaacttctc tttctatttt tatttttctg
     9541 ttgtatatct tattgataga ttctttgtcg agttttatgt ctctatacat atcagggcaa
     9601 tagcaacaaa cataatttct tttttttcga cgtttaaatt tcacaacttt ctctatgcta
     9661 atttctaatt tttttgtacc atcttttata aatttaatat cttctatgtt ctcgcttgaa
     9721 aaaatttctg tcaatcttaa agtatctaca gtttctctaa ttatttgctt tttataagaa
     9781 gagaatccat atccaaaaga aaggtttaat ttattaggga tgtagccatt tttatattta
     9841 aacatcttaa ttttttccaa ttcagcatta tctttaaatt tcaacttttc atacctaaaa
     9901 gttactttgc taaaaagagt atattctaca tcaatatatt cttttgcaat cttaaattta
     9961 tcgctacctt caatcacaat catagcctat ctccaatcgt tttatattaa aataaggcca
    10021 tagaacatgg tctaatagta ctcattagca ctataagaca atgttaacac aaaaagacaa
    10081 aaaaaggtta tttatttcaa ataacctttt tttttatata aaatataaag aattatattt
    10141 tattcttttt tatgatgtct ataatctgtg atatcagatt tagttctctt gaatttaagt
    10201 cttttaactc ttcatttata tcattttcta aatatgtttt tccagtggaa gtaatatctt
    10261 ttataagaac atctgcagaa atatttaata tatttacaaa ttttatgaat gttttaagac
    10321 ttggattctt tttaccactt tcgatttgag ttatatacca aggcgatact ccaaccatat
    10381 ttgcaaattc ttttgaagtt aaatttgctt ttttccgttc ttctttaagt cttttaccta
    10441 tataattcat tattgttact atttccctca cttatatttc tttgtattta atgatgcact
    10501 ttattaactc tagcattgca atttgttctt tttcagttaa tttattaatc atttcataac
    10561 attctaaagg tacagagtta tcaacgcctt ctgttgtaat aatatttgca aataacaatt
    10621 caagtggtat gtctaaagca gtagcaattt tcattataat attgatagat gtatttgtct
    10681 cagctctttc aatctgacca atatatgttg gatgtaaacc tgccttttct gctagaaact
    10741 cttgactata tcccaatttt gttctataga cttttattct atctcctatt atttttccta
    10801 tatttgccaa ttattatccc tccactttag attatattca tagtttaagt tgttttatat
    10861 agactattta tactttttta gtctaaatat agactatata tgtttttata ctgaaataag
    10921 tgctatttat ttttgtatag tctgtatcta caataaacaa atatggataa atcatatttt
    10981 taattagaga aatattttca taatttcata gccatactac aataataaaa cgaattaata
    11041 atatgttaat tatagcattt tttattgtaa ttacatatta gatatgt
//
LOCUS       DBGJFF010000125        11025 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.127, whole genome shotgun sequence.
ACCESSION   DBGJFF010000125 DBGJFF010000000
VERSION     DBGJFF010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11025
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.127"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(35..982)
                     /locus_tag="ACOIAF_003109"
     CDS             complement(35..982)
                     /locus_tag="ACOIAF_003109"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HGZ2670107.1"
                     /translation="MFYFLKVKVGDIMDEVKEFLVLEEEEEVRLDIYLAEQLGDMSRS
                     YIQKIIKDGKVKVNNKVEKARYLVKEEDNIVIEIPEPKVLEVLPQDIPIDIVYEDDDV
                     LIINKPQDMVVHPAPGNYENTLVNGILYHCKDKLSSINGVIRPGIVHRIDKDTSGLLM
                     IAKNNYAHNFLAEQLKEHTITREYEFICYGVVKEDKITVDKPIGRNPKDRLKMAVVKD
                     GKNAITHFEVVERFDKFTHMRARLETGRTHQIRVHALFINHPLLGDYVYGPKETKFKL
                     KGQTLHAKKLGFIHPTTKEYIEFNSELPEYFKEVIKKIK"
     gene            complement(951..1397)
                     /gene="lspA"
                     /locus_tag="ACOIAF_003110"
     CDS             complement(951..1397)
                     /gene="lspA"
                     /locus_tag="ACOIAF_003110"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HGZ2670108.1"
                     /translation="MLYILIIILLIGLDQLSKIWVLNNLVDVSTIPIINNVFHLTYVE
                     NRGAAFGLLQNNQWIFIIVALLATVFGLYYLNTRKVHIFGRLGIILIISGALGNLIDR
                     VRLGFVVDYFDFRIIWEYVFNIADVFVVVGTVFLCIYVLFFESKSR"
     gene            complement(1707..2486)
                     /locus_tag="ACOIAF_003111"
     CDS             complement(1707..2486)
                     /locus_tag="ACOIAF_003111"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HGZ2670109.1"
                     /translation="MKKIIWGICICLLSIVGVYTYNHNTHNQDSSKEAYSLNDNIKDD
                     YEDVIIKKGNEDEKILALTFDDGPDEDFTPQVLDILKKNDVKATFFVVGEKVEYNKEL
                     LKRQYDEGHEIGNHTFTHINVAKNSYSSVEKEITDTQNIIKSVIGVEPKIFRPPYRAM
                     SKSVCDIIVSKGMNIILWSNLDPRDWSNPGVDAIINTILTKVQNGNIILLHDYNTKRN
                     DKSQTIQALEVVIPKLKERGYKFVTVSELIQHLDKNKQVQK"
     gene            complement(2541..3023)
                     /locus_tag="ACOIAF_003112"
     CDS             complement(2541..3023)
                     /locus_tag="ACOIAF_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HGZ2670110.1"
                     /translation="MDTKKYKDKLLKERENLTNLVEDMKDNTLFGDTTKHTSEKYSSG
                     ELSSYDNHIGDMGTDLYMQNMQNSLINHEEGRLYQIDLALSKIENGTYGICDLCHNQI
                     DLERLDILPDTSLCNDCAKKENNLLGDAMQNPDVEDSNFYSEDLTNLTDLNKNGILRD
                     "
     gene            complement(3289..4710)
                     /locus_tag="ACOIAF_003113"
     CDS             complement(3289..4710)
                     /locus_tag="ACOIAF_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HGZ2670111.1"
                     /translation="MVSFLGSIVVLIIGYFVYGTFVEKVFGINDKNQTPAIACRDGVD
                     YVPMKWKRIFLIQFLNIAGLGPIFGAIQGALFGPSAFLWIVFGTIFAGGVHDFASGYL
                     SMKNKGTSASELVGLYLGNGAKIAMRIFSVILLVLVGVVFVTGPAGLLKTLTGVNAQI
                     WVGVIILYYIMATVLPIDKLIGKIYPLFGAALLIMAVGVAGGLIIKGYNIPNINLQNM
                     NPNGTPLFPYLFITIACGAISGFHATQSPLMARCVEKESEARPVFYGSMVAEGIIALV
                     WAAAAMSYFHGIPQLNVIFNDGGAATVVNTVSVGLMGPIGGALAILGVVACPITSGDT
                     AFRSARLTIADAMNYNQDAVKNRFLIALPLFAVGVALTFIDFNIIWRYFSWANQTLAM
                     IMLWTGSAYLVKANKNHYITTLPALFMTVVTFSYIMQAKEGFRLPVNISNGIGVIVAI
                     ILGLLFFKKAKHIKEQNNHKLAS"
     gene            complement(4856..5626)
                     /locus_tag="ACOIAF_003114"
     CDS             complement(4856..5626)
                     /locus_tag="ACOIAF_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HGZ2670112.1"
                     /translation="MRAIIVEDEFPARKELRYFIENKSGIEVVSEFTNGIEVLDFIQE
                     NKIDVIFLDINIPHLDGMLLAKTLNQFKSRPKIVFITAYESYAVDAFSLDVFDYILKP
                     YSEERIISMLNKLEKSEMSDIELSNGNSNLYKYKKEAVNQEIEEITHKISLWKGDKLV
                     VIDIDDIYYCEANERQTFIYTEKEKFILKEGISEVENLINDKTFFRTHRSYIVNLTKV
                     KEIIPWFNNTYILKLKNSDYEVTVSRSKVKEFRLLMHI"
     gene            complement(5631..6833)
                     /locus_tag="ACOIAF_003115"
     CDS             complement(5631..6833)
                     /locus_tag="ACOIAF_003115"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HGZ2670113.1"
                     /translation="MKTSILIALIEKTSLIVVLFLLITKLKIFKQIFQKEEYSFNDLV
                     CIALVFTFLAIFGTYSGINYMGSIVNTRIISIVSGGILFGPMVGITAGVFSGIHRYFM
                     DIGGITSVPCLLSSILAGVLSGFLYKRIPKQHRVMYGILVGMISESFTILLIYLISYP
                     HSLAIQIIGGIYLPLIVGQIGIGFVISIVEGIEKDKKDIEARNKAEIKALQRQINPHF
                     LFNSLNTIASFIRFSPDKARELIINLSTYLRYNLEYSDNLIDINKEIEQVKSFVEIEK
                     ARFGELLTVSYDIEDVNIKIPSLIIQPLVENAIIHGILESGRAGVVKISIKKLPYSLE
                     NTVRISIEDNGIGISEEIINNVYQDNMPENKIGLYNVHLRLKLMYGRGLNIRRIDNGT
                     LIVFYVKE"
     gene            complement(7421..8422)
                     /gene="trpS"
                     /locus_tag="ACOIAF_003116"
     CDS             complement(7421..8422)
                     /gene="trpS"
                     /locus_tag="ACOIAF_003116"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HGZ2670114.1"
                     /translation="MSEKKVIFSGAQPSGKMTLGNYLGAIKNWTELQDNYDCYYSIVD
                     LHAITVPQDPKVLRANTIELLAQYIACGLDPEKNTIFIQSHVKEHVELMWVLNTMTYM
                     GELSRMTQFKDKSQKSEANLNAGLFTYPVLMAADILLYQTDLVPVGDDQKQHLELARD
                     LANRFNNRFSPTFVVPEGYYPKGGARVMSLQEPTKKMSKSDSNENAFILLADDSDSIK
                     RKIKRSVTDSLGVVKYNDEQPGIKNLIDIYSNLSKKSVEEIVNMYEGKGYGIFKEDVA
                     EVVSEALRPIREKYVDLLNNKDYLEKIYSMGAEKAEKQARKTLRKVYKKVGFIERRY"
     gene            complement(8445..9143)
                     /locus_tag="ACOIAF_003117"
     CDS             complement(8445..9143)
                     /locus_tag="ACOIAF_003117"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HGZ2670115.1"
                     /translation="MNVIGIIGAMDEEVSILVDLMDIRETIKKASLEFYKGILEGKNV
                     VLVKCGIGKVNSALCAQILISEFKVDAIVNTGVAGALNEKLDVNDIVISTDAIQYDVD
                     TTAFGDPKGVIPRMKTSVFKADERLIDAAYKSSVEEVKTHKVLKGRVVTGDKFINSKE
                     LKEELVNDFGGYCGEMEGGAIAHVCYLNNTPFVIIRAMSDKADGSADVTYDVFVHDAA
                     NNSKDIVLNMLKSI"
     misc_binding    complement(9186..9426)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(9517..10920)
                     /locus_tag="ACOIAF_003118"
     CDS             complement(9517..10920)
                     /locus_tag="ACOIAF_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HGZ2670116.1"
                     /translation="MEKKKIGLWSLVFMNVSALYGIRWIAKSTASSFGLGLGAIPMWL
                     LFSFIYFVPGALICAELASTYQKGDGGLYDWVKEAFGEKYGFLVSWLNWTAKIFWYSS
                     FLTFLAVNVSYTIGNPALADNKMFVLILSLIIFWALSLIATKGISFTKIFTNTGALGS
                     TIPSAILIIFSFVAVFVLKKHDIASTYTIASMTPKLNMDAFVAISAIMFGLAGAETAA
                     NFITEIDKPEKNFPKAILISAGIVASLYVLGSIAITMIIPPDQITASKGVLDALSAVC
                     ASLGIGSWLVQLIAFGIAFSVLGAIVLYIASPIKMLFGSVKKGIFPDSLVEVNEHKIP
                     SKAVILQAIIVTIILLVTTLMPSVDAIYNVLVTMTALTALFPYVLLYASYIKLRKERP
                     DEIRPYTMAKSTSTCLGLAKMVLVVTVVGILLSAAPVMPTFAENVIYEIEMIGGGLLV
                     ILSGLWLWNRYEKKTAK"
BASE COUNT         3660 a         1950 c         1142 g         4273 t
ORIGIN      
        1 aacaaaaaat gatttataag tattatacaa atggttattt tatcttttta ataacttcct
       61 taaaatactc aggtaattct gaattaaatt cgatatattc cttagtagta ggatgtataa
      121 atcccaactt cttagcgtgc aaagtttgtc cttttaattt aaatttagtt tcttttggac
      181 catatacata atcacctaaa agtgggtgat ttataaacaa agcatgaact cttatttgat
      241 gagttctacc agtttcaagc ctagctctca tatgtgtaaa cttatcaaat ctttctacaa
      301 cttcaaagtg agtgattgca ttttttccat cttttactac agccattttt aatctatcct
      361 taggatttct ccctataggt ttatcaacag ttattttatc ttctttaacc acaccatagc
      421 atataaattc atactcccta gttatagtat gttcctttaa ctgttctgct agaaaattat
      481 gtgcataatt gttcttagct atcataagaa gaccagaagt atctttgtct attctatgaa
      541 ctataccagg tctaataact ccatttattg atgacaactt atctttacaa tgatataaaa
      601 tcccgtttac taacgtattt tcatagtttc caggagcagg atggacaacc atatcctgag
      661 gtttatttat tattaagaca tcatcatctt catatacaat atctattgga atatcttgag
      721 gtaaaacttc taatacttta ggttctggta tctctataac aatattatct tcctctttaa
      781 ccagatatct agctttttct actttattat taacctttac ttttccatct tttataattt
      841 tttgtatata acttcttgac atatcaccta attgttctgc tagataaata tcaagtctaa
      901 cttcttcttc ctcttcaagt actaaaaact cttttacttc atccattata tcacctactt
      961 ttactttcaa aaaataaaac atatatacat aaaaacacag ttcctacaac tacaaataca
     1021 tcagctatat tgaatacata ttcccatata attctaaagt cgaagtaatc tactacaaaa
     1081 cctaatcgta ctctatcaat tagatttccc aatgcaccag atataattaa tataattccc
     1141 aaccttccaa atatatgtac tttccttgta ttaagatagt atagtccaaa tactgttgca
     1201 agtaatgcaa caattataaa tatccattga ttattttgta ataatccaaa tgctgcacct
     1261 ctattttcga cataagttaa atgaaataca ttatttatta ttggtattgt tgatacatcc
     1321 accaaattat tcaataccca tattttagac agttggtcta aacctatgag tagaattatt
     1381 attaatatat atagcaatga tttttccccc tataaattat ttaaaaacca attttatttg
     1441 aagcacacta ttattattat acaaaaaaaa tgctctaaag agcaacttta ttaaaattgt
     1501 attttcttaa taaagctaga acaaaattta aaatagtact acttttttta tattgtagat
     1561 ttctacatat ttaaaatcat atcattatgt ataaaaaatt taaaaatacc atactaatca
     1621 atattttttg tgtattttat agattaaaga ttaatgatgt ctgtaattta aacttacaat
     1681 caccactaat cttcaaacaa atatttttac ttttgcactt gcttattttt atctaaatgt
     1741 tgtataagtt cagatactgt tacgaattta tatcctctct cttttaattt aggaattact
     1801 acttctaaag cttgtatagt ttgtgattta tcatttcttt tagtgttata atcatgtaaa
     1861 agtattatat taccattttg gacctttgta aggatagtat ttataattgc atctactcca
     1921 gggttagacc aatctctagg gtctaagttt gaccaaagaa ttatattcat gcctttagaa
     1981 actataatat cacagacact tttactcatg gctctatatg gaggtctaaa tatctttggt
     2041 tctactccta ttacactttt tataatattt tgcgtatctg ttatttcttt ttcaacacta
     2101 ctataactat tttttgcaac atttatatgt gtaaatgtat gattccctat ttcatgcccc
     2161 tcatcatatt gtctttttaa taattctttg ttatattcta ctttttctcc aactacaaaa
     2221 aaagttgctt ttacatcatt tttctttaat atatcaagta cttgtggtgt aaaatcctca
     2281 tctggaccat catcaaaagt caatgctaaa attttttcat cctcatttcc tttttttatt
     2341 attacatctt cataatcgtc ttttatattg tcatttaaac tatatgcttc ttttgaacta
     2401 tcttgattat gtgtattatg attatatgta tatacaccta ctatagataa taaacatata
     2461 catatacccc atattatttt cttcatgtca ttacctcttt tctaattcat tctagcaata
     2521 tattttataa tgtcagtttt ttaatctctc aatattccat ttttatttaa atcagttaaa
     2581 tttgttaggt cttctgaata aaagttagaa tcttctacat cagggttttg cattgcatca
     2641 cctaataaat tattttcttt ctttgcacaa tcattacata aacttgtatc tggcaatata
     2701 tccaatcttt ctagatctat ttgattatga cataaatcac aaatcccata tgttccattc
     2761 tctattttag ataatgctaa gtctatttga tacaatcttc cctcttcatg atttattaga
     2821 ctgttttgca tattttgcat atataaatct gttcccatgt ctcctatatg attatcataa
     2881 ctagataatt ctcctgatga atacttttca cttgtatgtt tagttgtatc tccaaataaa
     2941 gtattatctt tcatatcctc tactaaatta gttaaatttt ctctttcttt caataattta
     3001 tctttatatt ttttagtatc catttttaac acctctaatt ttaatattta catcataaaa
     3061 atagtatgtg gaaaagtgaa ttaaacatac aataaaaata agatttgatt cgaaaaatag
     3121 aatgtctcaa aatgaaattt ttagttcatt taaccctctt ttttatataa aatcaaatat
     3181 atttttatcc tttcagcaat aaaaaagaga cttatctcta ttttgagata gcctcttttt
     3241 tgaacataat ttaaatacaa ttttttaatt tttatagtta ttattttact aacttgccaa
     3301 tttatgatta ttttgttcct taatatgctt tgctttttta aagaataaaa gaccaagaat
     3361 tattgctaca ataacaccta ttccattaga tatattaact ggtagtctaa atccttcttt
     3421 tgcttgcatt atataactaa atgttactac agtcataaaa agtgctggta atgtagttat
     3481 ataatggttt ttatttgctt tgactaaata tgcagaacca gtccacaaca ttatcattgc
     3541 aagtgtttga tttgcccaag agaaatatct ccaaattata ttaaaatcta taaatgtaag
     3601 agcaacacca actgcgaata atggtaaagc tattaaaaat ctatttttga ctgcatcttg
     3661 attataattc attgcatctg ctattgtcag tctagcactt ctaaatgctg tatctccaga
     3721 agttatagga catgccacaa ctccaagtat agctaaagct cctccaatag gccccattaa
     3781 tccaacagat actgtattta ctactgttgc tgctccacca tcattaaata tcacattaag
     3841 ttgaggtatg ccatggaaat atgacatggc tgctgctgcc caaactaaag ctattatacc
     3901 ttctgcaacc attgacccat aaaatactgg tcttgcctca ctctctttct caacacatct
     3961 cgccataagt ggtgattgag ttgcatgaaa acctgatatt gctccacatg ctattgttat
     4021 aaataagtat gggaataaag gtgttccatt tggattcata ttttgtaaat ttatatttgg
     4081 tatgttatac ccctttatta ttaatcctcc agctacacct actgccataa ttaataatgc
     4141 tgctccaaat aaaggatata ttttaccaat caatttatct attggtaaaa cagtagccat
     4201 aatataataa agtattatca ctccaaccca tatttgtgcg tttacacctg tcaatgtctt
     4261 taataatcct gctggacctg ttacaaatac aactccaacc aatactaaaa gaataacact
     4321 aaatattctc attgctattt ttgcaccatt tcctagataa aggcctacca actctgatgc
     4381 agatgtacct ttgtttttca ttgatagata tcctgaagca aaatcgtgta ctccaccagc
     4441 aaagattgtt ccaaacacaa tccataaaaa tgctgatggt ccaaataatg ccccttgtat
     4501 agctccaaat atcggaccta accctgctat atttagaaac tgaattaaaa agattctctt
     4561 ccatttcatt ggcacataat ctactccatc cctacaagct atagctggag tttgattttt
     4621 gtcattgatt ccaaaaacct tttctacaaa agtaccatag acaaaataac ctattataag
     4681 tactactatt gaacctaaaa agcttaccat aaattcatcc ccttagtttt tatataattg
     4741 ctttataaat ttttatgagt ttaagaaaac tatttccagt tcaaatagta atactttttt
     4801 atgctaatat aaatattaat tacattaagt gcatatttaa gtacatgaaa tgactttata
     4861 tatgcattaa cagcctaaat tcttttactt tgcttctact tacagttact tcatagtcac
     4921 tattttttaa ttttaatatg tatgtattat taaaccatgg tataatttct tttactttgg
     4981 ttaagtttac gatataagac ctatgtgttc taaaaaatgt tttatcattt ataagatttt
     5041 cgacttctga tatgccttct tttaatataa acttttcttt ctcagtatat ataaatgtct
     5101 gcctttcatt agcttcacag taatatatat catcaatatc tattacaact aatttatcac
     5161 ctttccaaag gctaatttta tgtgttattt cttctatctc ctgatttact gcttccttct
     5221 tatatttgta taaattacta tttccattag atagttcaat atcactcatc tcactttttt
     5281 ctaatttatt cagcatagaa ataattctct cttcagaata tggtttcaat atataatcaa
     5341 atacatccaa agaaaaagca tctactgcat aactttcata tgctgttata aaaacaattt
     5401 taggtcttga tttaaactga tttaatgttt ttgcaagtag cattccatca agatgaggta
     5461 tatttatatc taaaaaaatt acatctattt tattttcctg aataaagtct aacacctcta
     5521 taccattggt aaattcactt actacctcga ttccactttt attttcaata aaatatctca
     5581 attcctttct tgctggaaat tcatcttcca ctattatagc tctcatactt ctactccttt
     5641 acataaaata ctatcaaagt cccattatct attcttctta tattaagccc tcttccatac
     5701 atcagtttaa gccttaaatg tacattataa agcccaatct tattttctgg catattatct
     5761 tgatatacat tatttataat ttcctcactt atacctatac cattatcctc tattgatatt
     5821 ctcacagtat tttctaatga atacggtaac ttttttatgg atattttcac tacaccagct
     5881 ctaccacttt ctaaaatacc atgtattatt gcattttcca ctaatggttg tataatcaaa
     5941 cttggaatct ttatatttac atcttctata tcataagata cagttagtaa ttctccaaac
     6001 ctagcttttt ctatctctac aaaagattta acttgttcaa tctctttatt tatatcaatt
     6061 aagttatcac tatattcaag attatatctt agataagttg ataaatttat tattaactct
     6121 ctagccttat ctggactaaa tcttataaat gaagctattg tgtttaggga attaaacaaa
     6181 aaatgcggat taatttgtct ttgaagtgct tttatttctg ctttatttct cgcttctata
     6241 tcttttttat ctttctcaat cccctctact atagatataa caaatcctat tccaatctgc
     6301 ccgactatta aaggtaaata tattccaccg attatttgaa ttgctaaaga atgtggatat
     6361 gatattaagt atataagtaa tatagtaaaa ctttcagaaa tcatgcctac caatattcca
     6421 tacattactc tatgttgttt tggtatcctc ttatatagaa atcctgataa tacacctgct
     6481 agtatacttg ataaaagaca tggaacagat gttatccccc caatatccat aaaatatcta
     6541 tgaatccctg aaaaaactcc tgctgtaata cctaccattg gaccaaataa aatgccacct
     6601 gatactatag atattattct agtgtttact attgacccca tataatttat accactataa
     6661 gttccaaata tggctaaaaa agtaaaaact aatgctatac agaccaagtc attaaaacta
     6721 tattcttcct tttgaaatat ctgcttaaat atttttaact ttgttattag aagaaacaat
     6781 actacaataa ggcttgtctt ttctattaat gcgattaaaa ttgatgtttt cataatcccc
     6841 ctttaaaaaa taaaacatta cttaatttaa gtttattatt cttataataa attagataca
     6901 taaaaataaa ttccataatt attgtatcat atcacctata tataaacata aattcattta
     6961 attatataaa tttaaaaaat atttttttga tatattattg acaattttca ttataagagt
     7021 taaaattaac tcataaatta catattgggt ataaaataca atgtatgtta gaagttgttt
     7081 cagaaggcta tggttttctg atttttttat gctcagtagt gatatgcaaa aaagacaaag
     7141 ttgacatcag ttaactttta cctttattct actttcccca aagtaagaat aatctaaccc
     7201 ctttgagatg agcaaaagct catctttttt tattatcaaa agatgctgtc tcaaaataga
     7261 gataatcctc tttttcattg ttgaaatgat taaaatatat tttatttcat acaaaaaaga
     7321 gtgcctcatg aacaaaagtt cactttgaga ctctctcttt catatcatat acctttttaa
     7381 taactttaaa atttatttat caagtaaaga tagctaataa ttagtatctt ctctctataa
     7441 atccaacttt tttataaact tttctaagag tttttctagc ttgtttttcg gctttttctg
     7501 ctcccatact atatattttt tctaaataat ctttattatt aagaaggtca acatattttt
     7561 ctcttatagg tcttaaagct tctgatacta cttcagccac atcttcttta aatatgccat
     7621 aaccctttcc ttcatacatg tttacaatct cttctacact ctttttactt aaattagaat
     7681 atatatcaat tagattttta atacctggtt gttcatcatt gtattttaca acacctaaag
     7741 aatcagtaac acttcttttt atctttctct ttatagaatc tgaatcatct gctaatagta
     7801 taaatgcatt ttcatttgaa tcggatttac tcattttttt agttggctct tgcaaactca
     7861 tcactcttgc tccacctttt gggtagtatc cttctggaac tacaaaagta ggtgaaaatc
     7921 tattattaaa tctatttgct aaatcacgag ctaattctaa atgttgtttt tggtcatctc
     7981 caactggaac taggtcagtt tgatacaaca atatgtcagc agccataaga actggatatg
     8041 tgaataaacc tgcatttaga tttgcttcac tcttttgtga tttatctttg aattgagtca
     8101 ttctacttaa ttcacccata taagtcatag tattcaatac ccacataagt tcaacatgct
     8161 ctttgacatg tgattgtata aatatagtat ttttttctgg gtctaatcca caagctatat
     8221 attgagcaag taattctata gtattggctc ttaaaacttt agggtcttga ggtactgtta
     8281 ttgcatgtaa atcaactata ctataataac aatcataatt gtcttgaagt tcagtccagt
     8341 tttttatagc tcctaaataa tttcctaaag tcattttgcc tgatggctgt gctccactaa
     8401 atataacttt tttttcactc atttttttgt acctccttat atatttatat tgattttaac
     8461 atatttaaaa ctatgtcttt agagttattg gcagcatcat gtacaaatac atcataagta
     8521 acatcagcac ttccatctgc cttatcagac attgctctta ttattacaaa tggagtatta
     8581 tttaaatagc atacatgagc tatagctcca ccttccattt ctccacaata tccaccaaaa
     8641 tcatttacta attcttcttt taattcttta gaatttataa atttatctcc tgtaactact
     8701 cttcctttta atactttatg agtctttact tcttccacag aagatttgta agcagcatct
     8761 ataagtcttt catctgcttt aaatacagaa gtcttcattc ttggtataac tcccttaggg
     8821 tctccaaatg ctgtagtgtc tacatcatac tgtatagcat cagttgatat tactatatca
     8881 ttaacatcaa gtttttcgtt taaagctcct gcaacacctg tattaacaat agcatcaacc
     8941 ttaaattcgc ttattagtat ctgggcacaa agagcagagt taaccttacc tatgccacac
     9001 tttactaata ctacattctt accttccaaa attcccttat aaaattctaa gctagctttt
     9061 tttatagtct ctctaatatc cattaaatca actaaaatac taacttcttc atccattgca
     9121 cctataattc ctattacgtt catttttcct cctaaatttt ctatattaaa aaatccgtcc
     9181 taaatcaata ggacggatat attttccgtg gtaccaccta aatttatata cactcaaaat
     9241 tgaatatata tatcttaata tatgctataa cgtgcataca cgatatcaaa tactctgtaa
     9301 tcttaaatta catttcattt tactcctcaa agacccattc aataaatact ttttattaag
     9361 ttctcagcat ccttaattct ctgtgcaaaa atatatttat ttactatttc ttatctcagg
     9421 atttaattta ttaatttctt tattttataa tattccttac actataaatt gtcaactata
     9481 tattataata tccaaaagaa atttttaaaa tacattttat ttagctgttt tcttttcata
     9541 tctattccat aaccatagac cagataatat aactagaaga cctccaccaa tcatctctat
     9601 ctcatatata acattttcag caaatgtagg cataactggt gctgcagata ataatatacc
     9661 cactacagtt acaactaaaa ccatttttgc taaacctagg caagtacttg ttgattttgc
     9721 catagtataa ggtctaattt catctggtct ctcttttctt agtttaatat aagaagcata
     9781 taacaataca tatgggaata atgcagttaa tgctgtcata gttacaagaa cattgtatat
     9841 agcatcaaca gaaggcatta atgtagtcac caatagtatt atagtaacta ttattgcttg
     9901 aagtataact gctttagaag gtatcttgtg ttcatttacc tctactaaac tatctggaaa
     9961 tattcctttt ttaacactac caaataacat ttttattggt gatgctatgt aaagaactat
    10021 tgctcctaat actgaaaaag ctataccaaa agctattaat tgaaccaacc aagaaccaat
    10081 tcctaaagac gcacaaacag cagaaagtgc atctaataca cctttagaag cagtaatttg
    10141 gtctggtggt attatcattg ttattgcaat tgaacctaat acatataatg aagctactat
    10201 tccagctgat attaatattg ccttagggaa gttcttttca ggcttatcta tttctgttat
    10261 gaagttagca gcagtctctg ctcctgctaa accaaacatt atagctgata tagcaacaaa
    10321 tgcatccata ttaagttttg gagtcattga agctattgtg tatgtagaag ctatatcatg
    10381 ttttttcaac acaaatactg ctacaaatga aaatattata agtatagcac ttggtattgt
    10441 tgaacctaat gcacctgtat tagtaaatat ttttgtaaaa cttattcctt tagttgctat
    10501 aagagataat gcccagaata ttattaaaga taatataagt acaaacatct tattatcagc
    10561 aagggctgga tttcctattg tataagaaac atttactgct aagaaagtta aaaatgaaga
    10621 ataccaaaat attttagctg tccagtttaa ccaagaaact aaaaatccat acttttctcc
    10681 aaaggcttcc ttaacccaat cgtaaagacc tccatcacct ttttgatatg tagaagctaa
    10741 ttcagcacaa attaaagctc ctggtacaaa gtatataaat gagaataata accacatagg
    10801 tattgcacct aatcctaaac caaagcttga tgctgtagat ttagctatcc atcttatacc
    10861 atataaagct gatacattca taaataccaa actccatagg cctatttttt tcttttccat
    10921 cctaatttaa tttcccctct ctattaatta ttttaaattt aatttcaaaa ttaataaaat
    10981 agattcatta ttattaaact gatatttttc aaaatatttg catat
//
LOCUS       DBGJFF010000126        10914 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.128, whole genome shotgun sequence.
ACCESSION   DBGJFF010000126 DBGJFF010000000
VERSION     DBGJFF010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10914
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.128"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            158..832
                     /locus_tag="ACOIAF_003119"
     CDS             158..832
                     /locus_tag="ACOIAF_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGZ2670117.1"
                     /translation="MNNKILVVDDEEGIITLLKDYFEMNGYEVYTAQSGKDALKKISK
                     NPDIILLDINMPEIDGIEVCNRIRDYVSCPILFLTARIENTDKINGFRAGADDYIVKP
                     FDLDELGARISAHLRREQRKSNQTKIKFYNDLTIDYEKRLICIYNNQISLSKKEFDIV
                     ELLSMNPGQVFDKEKIYDRVWGYNSNGNSDVIMEHIRKIRIKLSKHTLENYIETVWGV
                     GYKWIK"
     gene            820..1980
                     /locus_tag="ACOIAF_003120"
     CDS             820..1980
                     /locus_tag="ACOIAF_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGZ2670118.1"
                     /translation="MDKINNMSLKKTFFVLTLSSLIIASVLTTFAYILLNNLYNSIQD
                     KYLKTPINTGIITIVQNNVNQFSDYDKTLLNIINILQLVLPLIFFIGLLLLADIIFYK
                     VKLKTPIEILNKGAMEISNNNLDFCLEYNNNDELGNLCNAFEKMRSELNKNNIKMWTM
                     INDRKQLNAAFSHDLRNPLTVLKGYSNYLTKYIPTGKLSDEKILSTTQLMSEHINRIE
                     YYVENMSNAQRLEDLVVSKSMSNINKFIENLDENISIIAKQEGKSFTLKSQINNINLF
                     FDENIILRVVENIISNAFRYARNNVSILIYLEQELLTFVIEDDGIGFSKESLKLALRP
                     FYRDKTLNDSNAHFGMGLYISKILCEKHGGSISIENNSTNSAKITAKFSTRN"
     gene            2218..2724
                     /locus_tag="ACOIAF_003121"
     CDS             2218..2724
                     /locus_tag="ACOIAF_003121"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HGZ2670119.1"
                     /translation="MTFSRIVFALLMLSSTFCSLPFWICYFWCGISDILDGLIARKLR
                     QQSGIGAKLDSIADFIFAVTILVTVGKNIALPMYLWWCIVAIALLRLISYSIGFFKYR
                     TFSSLHTLMNKITGISIFAFPLLYICFGLDVAGIMICIVAFLSSFEEIAIMIKSKELN
                     RDIKSIFM"
     gene            3010..3678
                     /locus_tag="ACOIAF_003122"
     CDS             3010..3678
                     /locus_tag="ACOIAF_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2670120.1"
                     /translation="MIIKAKQLSKIYGSNNNKVIALNNVNLEINSGEFVSVIGPSGSG
                     KSTLLHILSGLDNPTSGQVLLDDKDIYKHTEKELSALRRKSFGFVFQQFNLLPVLTAS
                     ENISMPVLLDKKQPDKGYLNEISSLLGIADRLNHLPHELSGGQQQRVAIARALIAKPD
                     IIFADEPTGNLDSKSGSEVMNLLIKTSKQFGKTLVVITHDDRIAKLADRKISIIDGVL
                     MEVK"
     gene            3681..6149
                     /locus_tag="ACOIAF_003123"
     CDS             3681..6149
                     /locus_tag="ACOIAF_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2670121.1"
                     /translation="MKNYLSLSIKELKSQKLMTTFIIIAIVLSTIMTTVVGQSIGILQ
                     NLRIEQARSFNGDRHVSFHQLTKNQVDDIKKDDRVYQAGTSITIGSSKIKDSGISVLV
                     KEYDKTGLSNYPKLMKLKSGHLPKDKNEIALDENTLKLMGIKPRLGVTIPMNLDISLL
                     NDTIPPYNYTANFKLSGILEDDYTGYVSGIVNGIVGKGTSENLLPKRYILSSLDFKIK
                     QQEKFQEIVNQLAKKINLSHNSIQYNWIYLNALKIQFEKDENSSNSDGISMIILVSLF
                     VALLVLLASGLVIYNILKISVTKKIKEYGCLRAIGAEPNQIYKIVILQILILCTVAIP
                     IGAVIGIISSKGITGMVTNILNPDILLANDNKEITELIHKNTTAYMFPLVLSTNVSLI
                     FSFISALPSAIYASHVSPKIAMAGSTTKIKRKIKREKTIKNFERHLAWLNLKRNKGRT
                     IITILSLFMSITVFVALSEFSNVLDVSRSVSNLKEGDFSLTNEISGFDKSTLDKIAKM
                     KNVNRTSFIKYSEYKQGEIDTDINFENSGEMLKIIGIDEQTLKDLMPSITDSILEDFK
                     NGSICFIKNPIAISTPGVKTRHTNLKPKDSITINKKQLDIYSTVDKMFFLQGNGWING
                     VDVIVYDSVYNTLTNKNKFNQINIYAKDKSKLEQIRLSIEQICENNPGSHWISYIESD
                     KQLKESFKQIEFLAWAVILFVGLIGTLNIINTTHTNINTRTNEIGVKRAIGMSNSSLY
                     KMFLWEGVYYGIFAAIFGSIAGYASAIIINMATIEKLDFTNIPITSILQATIISVLAC
                     IIATLIPLRKVKKMNIIDCIDINL"
     gene            complement(6389..7558)
                     /locus_tag="ACOIAF_003124"
     CDS             complement(6389..7558)
                     /locus_tag="ACOIAF_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HGZ2670122.1"
                     /translation="MLYQVVDNNGNILYGTNPKKLFKTKEELFNNFINKTVRKGGYIH
                     TVPIKGDNGKIEGAVILFYQVKITFANIRGRFVFAVIIMALFSPFLYIVGFTRWLSKR
                     FVKNINQPLHLLIDASKKIKEKDLDFEIDYYSDNELGKLCSAFSEMKDELKGSLSAQW
                     KMEQERVEMVEALAHDLKSPLSIILGYTDALIGNNTDDNEKLHRYLTVIRENTEKSAA
                     LVQKMQYTSDLEKSNIQLNLVPINLPEFLRQKVQDYELQAHQKEVELILKMQGNIQSP
                     IQIDVDRLTRIFDNIISNSLQYTPSGGNISITVKDEKNCISYEICDSGRGFSSKDLKK
                     ALDKFYRGDEARQTKGGHSGLGLYIVKQLVEQLGGSVKIENSKSGGACVKFWHSI"
     gene            complement(7832..8524)
                     /locus_tag="ACOIAF_003125"
     CDS             complement(7832..8524)
                     /locus_tag="ACOIAF_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGZ2670123.1"
                     /translation="MSKILIIDDEMDLVMLLEDELKAKGHEVLIAYDGQAGIELSKQE
                     PDLIILDIMMPKMNGFEVCQAIRDDVLCPIIFLSAKQSETDKIKGLTFGGDDYVTKPF
                     GLRELMARIEANLRREKRSQYINEENKRSTLYFGKLCLYMKERAIKIDGKNIDLTKIE
                     YDIVELLALHAGQVFSREQIYEKVWECDSEGDSSTVVERVKKIRAKFSAVTPEKEYIS
                     TVWGIGYKWNKM"
     gene            complement(8579..9355)
                     /locus_tag="ACOIAF_003126"
     CDS             complement(8579..9355)
                     /locus_tag="ACOIAF_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HGZ2670124.1"
                     /translation="MIKLLSSEWLRAKRTAVQWLTLCMPIIFSLCVVFYLTTKSGSTQ
                     EFAFEGFFTVWTVFIIPIGVGILAGFIVQEEELAGNFNGFLCVGISRVRLYLGKFLFL
                     LFCLTICTFIATLILCIGMSIAVPSGATIWLFLSAAGLVVIGTLPLLAIHLWVSFAWG
                     MGASIGISIGGILMAAILGLTSVGEKVWAFVPWTWPVKLGMLPGIYFIKEAGTISTEA
                     FYSKVMQTASIELIVVAIGLIIFLIGGVIWFKMWEGRKSY"
     gene            complement(9352..10122)
                     /locus_tag="ACOIAF_003127"
     CDS             complement(9352..10122)
                     /locus_tag="ACOIAF_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HGZ2670125.1"
                     /translation="MNALQSELLKYKRTFMGKLIVFFPVSFAAYAFIMQSTLMQNPLS
                     QTTSWAWQSLLALIFNWWSFLFLPIGFALFATLVAFQEKKAGNYRALRTHNVSPMTLW
                     INKVIAMAVYSFISTLVLIFVTIITGLILKAGPVPFGQIIGASIVCWVVSLAILPLQL
                     WLATWKGMFLSMGVGALGMIFGVLAATKPFWIAVPWSWAVRMVCPIINIHPNGTILEA
                     GDPLLNTSVIPIGIVVSLVVFIVLTALTAAWFNRRDDK"
     gene            complement(10119..10823)
                     /locus_tag="ACOIAF_003128"
     CDS             complement(10119..10823)
                     /locus_tag="ACOIAF_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HGZ2670126.1"
                     /translation="MKDLILETKNLSKRYGEQMAVNNVSLQIERNSIYGLLGPNGAGK
                     STTLKMLVGLLRPTNGRIIFDRKPWKRESLAKIGSLIESPALYGNLTAEENLLVHTKI
                     LGIPKDKIYEVLEIVNLKNTGKKRASQFSMGMKQRLGIAIALLSDPELLILDEPTNGL
                     DPFGIQELRELIASFPKKGITVILSSHILSEVAQVVDNIGIINGGRLLFQGMPDPNEN
                     LEEFFTDVILKGGQNR"
BASE COUNT         3948 a         1742 c         1560 g         3664 t
ORIGIN      
        1 atttctaata atttcatcct tgatttattt aatatataaa aatatactaa ctttaatgat
       61 taagttttga aatataacta agaatttgat aatttattga gatttttata ctaaaatata
      121 catactatac aaacttaatt tagaagggag cattcttatg aacaataaaa tactcgtcgt
      181 agatgatgag gaaggtataa taacactctt aaaagattac tttgaaatga atggttatga
      241 ggtttacact gctcaaagtg gaaaagacgc attaaaaaaa atatccaaaa atccagatat
      301 cattctactt gatataaaca tgcctgaaat agatggaatt gaagtttgta atagaattag
      361 agattatgta tcatgcccta ttctatttct tacagctaga attgaaaata ctgacaagat
      421 aaatggtttt cgtgctggtg ctgatgatta tattgtaaaa ccttttgatt tagacgaact
      481 tggagctaga atatctgccc atttaagacg tgaacaaagg aagtcaaatc aaactaaaat
      541 taaattttat aatgatttaa caatagatta cgaaaaaagg ttaatttgta tatataataa
      601 tcagatttca ctttcaaaaa aggaatttga tatagtagag cttctctcta tgaatcctgg
      661 gcaggtattt gataaagaaa aaatatatga tagagtctgg ggatacaata gcaatggaaa
      721 tagtgatgtt ataatggaac acattagaaa aatacgaatc aaactatcaa aacatactct
      781 tgaaaattat atagaaacag tctggggagt tggttataaa tggataaaat aaataatatg
      841 tcattaaaaa aaactttctt tgttcttact ttatcttcac taattatagc atcagtactt
      901 actactttcg catacatttt acttaataat ctatataatt ctatacaaga taagtattta
      961 aaaacaccta taaatacagg tattataacc atagttcaaa acaatgtgaa ccaatttagt
     1021 gattatgata aaacattact taatataatc aacatacttc agcttgtact tccacttata
     1081 ttttttatag gacttttatt attagctgat ataatttttt acaaggtaaa attaaaaaca
     1141 cccatagaaa tactcaataa aggtgcaatg gaaatatcca acaacaattt agacttttgc
     1201 ttagagtata ataataatga tgaattaggt aatctatgta atgcttttga aaaaatgagg
     1261 tctgaactaa ataaaaacaa cataaagatg tggactatga ttaatgatag aaaacaactt
     1321 aatgctgctt tttctcatga tttaagaaat cccctaacag ttctaaaagg ttactcaaac
     1381 tatttaacaa aatacatacc aacaggtaag cttagtgatg agaagatttt atcaaccaca
     1441 caacttatgt ctgagcatat taacagaatt gaatactatg tagaaaatat gagtaatgct
     1501 caacgattag aagatttagt tgtttcaaaa tctatgtcga atataaacaa gtttatagaa
     1561 aacttagatg aaaatatatc tatcattgct aaacaagaag gaaaatcttt tacacttaaa
     1621 agtcaaataa ataatataaa tctatttttt gatgaaaata tcattcttag agtcgtcgaa
     1681 aatataatat cgaatgcttt tagatatgct agaaataatg tatccatact tatatatctt
     1741 gaacaagaac tccttacctt tgtaattgaa gatgatggta taggatttag taaggaatct
     1801 ctaaaattag ctttaagacc tttctataga gataagactt taaatgacag caatgcacac
     1861 tttggtatgg gtctttatat ctcaaaaata ttatgcgaaa aacatggtgg ctctatttct
     1921 attgaaaata attctactaa cagtgctaaa attactgcta aattctcaac aagaaattaa
     1981 aatgattttg taagctttca ctgttcttat ataagctttg ttagtttaga atttataaaa
     2041 tgtatttcaa tttataacca atatatatac tattttgtag aagttgtaag agttgcttca
     2101 attataatat tttggcaaaa ggcatttgaa acaataatag aaatatatat ttaaatatgt
     2161 agaatagtat gaaattagaa ttttggaggt gattatatta aaagttttgc taataatatt
     2221 acattctctc gtattgtgtt tgcattgcta atgcttagtt ctacattctg ttcactgcct
     2281 ttctggatat gttatttttg gtgtggaatt agtgatatac ttgatggtct tattgcccga
     2341 aagttgagac aacaaagtgg aataggtgca aagttagata gtattgctga tttcattttt
     2401 gcggttacaa ttttggttac tgtcggaaaa aatattgctc ttcccatgta tctatggtgg
     2461 tgtattgttg caatagcatt gttacggttg attagttaca gtattggttt ttttaagtat
     2521 cgcacatttt catcacttca tactttgatg aataaaatca ctggaatttc tatctttgct
     2581 tttccattac tctatatttg ctttggtctt gatgttgcag gaattatgat atgtatcgtg
     2641 gcttttcttt cttcttttga agaaatagca attatgataa aatcaaaaga attaaatcgt
     2701 gatataaaaa gtatatttat gtgaaaactt agaatttgtg acaaaaataa tttatataat
     2761 aatgaggaag taaaacataa aaaattatcc acaagtttat gtgtaaaata gttttaaagc
     2821 aatagagttg tattgtaaag cctttagtgc ataaattaca ttttaaatta aaaatgatac
     2881 tgaaactgct tatcagcact gcgaattatc cgcaaatagt gaagactttt tagatttaag
     2941 ctaaaagttg ggaaaaaatt gagttttaaa acttacactc ttcttatata gaaaaggaga
     3001 gtgtttttta tgattataaa agcaaaacaa ttatcaaaaa tatatggttc taataacaat
     3061 aaagttatcg cacttaataa tgtaaattta gaaataaact caggtgaatt tgtttctgta
     3121 attggacctt ctggaagtgg aaaaagcaca cttttacata ttttaagtgg attagataat
     3181 cctacctctg gacaagtgtt acttgatgat aaagatatat ataaacacac tgaaaaagaa
     3241 ctatctgcac ttagaagaaa atcctttgga ttcgtctttc aacaatttaa tcttttacct
     3301 gttcttacag catctgaaaa tatttcaatg cctgtacttc tagataaaaa acaacctgat
     3361 aaaggctatt taaatgaaat ttcatcatta cttggaattg cagatagact caatcatctt
     3421 ccccatgaac tttcaggcgg tcagcaacaa agagttgcaa ttgctagagc acttattgca
     3481 aaaccagata taatatttgc tgatgagcct acaggaaact tggacagtaa aagtggcagt
     3541 gaagttatga atcttcttat aaaaacttca aaacagtttg gaaagacttt agtggtaatt
     3601 actcatgatg atagaattgc aaaacttgct gatagaaaaa tttcaattat tgatggtgtt
     3661 cttatggagg tgaaatagaa atgaagaatt acctttcact ttcaataaaa gaattaaaaa
     3721 gccagaaact tatgactaca ttcatcatta tagcaatagt actatcaact attatgacaa
     3781 ctgtggttgg acagtcaatt ggtatcctac aaaatttaag aattgaacaa gcccgttctt
     3841 ttaatggtga tagacatgta tcctttcacc aactcactaa aaatcaagtt gatgacataa
     3901 agaaagatga tagagtttat caagctggta cttcaataac tattggcagt agtaaaataa
     3961 aggattctgg tatctctgta cttgtaaaag agtatgataa aacaggactt tctaactacc
     4021 caaaattaat gaaattaaaa tctggacatt tgcctaaaga taaaaacgaa atagccttag
     4081 atgaaaacac actaaagctt atgggtataa aaccaagact aggagtgact atacctatga
     4141 atttagatat ttcactttta aatgatacca taccaccata taattacaca gctaacttta
     4201 aattgagtgg cattttagag gatgattata caggatatgt aagtggtata gtaaatggta
     4261 ttgtaggtaa aggtacatca gaaaatctat tacctaagag atatatttta tcctctttgg
     4321 attttaaaat aaaacagcaa gaaaagtttc aagaaatagt aaatcaatta gctaaaaaaa
     4381 taaatctttc tcacaattca attcaatata attggattta cttaaatgct cttaagatac
     4441 agtttgaaaa agatgaaaat agttctaata gtgatggtat atctatgatt atattggtat
     4501 ctttatttgt tgccttattg gttttactgg cttctggact tgtaatctat aatattttaa
     4561 aaatctctgt tacaaagaaa attaaagagt atggatgttt aagagcaata ggagctgaac
     4621 caaaccagat atataagata gttattttac aaatcttaat tttatgtact gttgccatac
     4681 ctattggagc tgtaattgga attatatctt ctaaaggcat aactggaatg gtcactaata
     4741 tcttaaatcc agatatactt ctagcaaatg ataataaaga aattactgag cttatacata
     4801 aaaatacaac agcatatatg ttcccattag tattaagcac taatgtatct ttgatttttt
     4861 catttatttc agcattacca tcagcaatat atgcttcaca tgtctctccc aaaattgcta
     4921 tggctggtag tactaccaaa atcaaaagaa aaattaaaag agaaaaaaca ataaaaaact
     4981 ttgaaagaca tctagcatgg ctaaacttaa aaagaaataa aggaagaact ataataacta
     5041 tactatcatt atttatgagt ataacagtat tcgttgcact gagtgagttc tctaatgtat
     5101 tagatgtatc taggtcagta agtaatttaa aagaaggaga tttttcctta acaaatgaaa
     5161 tatctggttt tgataaatca acactagata aaatagctaa aatgaaaaac gtcaatcgca
     5221 cttcctttat aaagtactct gaatacaaac agggtgaaat agatacagat ataaattttg
     5281 aaaatagtgg tgagatgtta aaaatcattg gaattgatga gcaaacttta aaagacttaa
     5341 tgccttctat tacagattct atactagaag actttaaaaa tggttctatc tgttttataa
     5401 aaaatccgat tgctatttct acaccaggtg taaaaactag acatacaaat ttaaaaccta
     5461 aagatagtat cacaatcaac aaaaaacaac tagatatata tagtacagta gataaaatgt
     5521 tctttttaca aggtaatggc tggataaatg gtgttgatgt tattgtatat gattctgttt
     5581 ataacacctt gacaaataaa aacaagttca accagataaa tatttatgct aaagataaat
     5641 caaaattaga acaaataaga ttgtctattg aacagatttg tgaaaataat cctggctctc
     5701 attggatatc atatattgaa tcagataaac aacttaaaga gagttttaaa caaatagaat
     5761 ttctagcatg ggcagttata ttatttgtgg gattgattgg tactttaaac atcattaaca
     5821 caactcatac aaatataaat actaggacta atgagattgg agttaaaaga gctattggta
     5881 tgagtaatag tagcttatat aaaatgtttt tatgggaagg tgtttattat ggaatttttg
     5941 ctgctatatt tggttcaatt gctgggtatg ccagtgctat tattataaac atggcaacta
     6001 tagaaaagct tgatttcact aatataccaa ttacttcaat tttacaagct actattattt
     6061 ctgtacttgc atgtattata gctactctta ttccgttaag aaaagttaaa aagatgaata
     6121 taattgactg tatagatatc aatttataac tattttacag ataataatat aaattaaatt
     6181 atcaatgact gtctctattt tctttcaaat ctaactaata aatcttttta actataatat
     6241 aagctttaaa aaaggcctgt ctcaaaatag agataagcct tttttcattg tcgaaataat
     6301 gaaaatatat ttaacttcac aaaaaaagag tgcctcacga attaaaaatt tcgctttgag
     6361 acacccccct ttaatttaaa atatatagtt aaatgctatg ccaaaatttc acacatgctc
     6421 cacctgattt tgaattttct attttaacag aacctccaag ctgttccact aattgtttga
     6481 caatgtaaag cccaagacca gaatgtccac cttttgtctg cctagcttca tctcctcgat
     6541 aaaatttatc aagagccttt tttaaatctt tagagctaaa ccctctacca gaatcacaaa
     6601 tctcataaga aatacaattt ttttcatcct ttacagtaat actaatatta ccacctgatg
     6661 gagtgtattg cagactgttg gatatgatat tatcaaaaat tcgtgtcaac ctatcaacgt
     6721 cgatttgaat tggggattgt atattcccct gcattttcaa tataagttca acttcctttt
     6781 gatgagcctg taattcatag tcttgcacct tttgcctcaa aaattcaggt aaatttatag
     6841 gaacaagatt taattgtata tttgactttt ctaagtcaga cgtatattgc atcttttgga
     6901 caagagcagc acttttttct gtattttctc ttatcactgt aagataccta tgaagttttt
     6961 cattgtcatc tgtattatta ccaataagtg catctgtata tcccaaaata atagaaagag
     7021 gagattttaa atcatgtgct agagcttcca ccatttcaac acgttcctgc tccatcttcc
     7081 actgtgcaga taaagagcct tttaattcgt ctttcatttc agaaaaggca ctacaaagtt
     7141 taccgagttc attatctgaa taatagtcga tttcaaaatc aaggtccttt tcttttatct
     7201 tcttggacgc atcaatcaat agatgtaatg gttgattgat attctttaca aagcgcttag
     7261 aaagccatct agtaaaaccc acgatatata gaaatggaga aaacagagcc attataataa
     7321 ctgcaaatac aaatctccct ctaatatttg caaaggtgat tttcacttgg tagaatagaa
     7381 taacagctcc ctcaatcttt ccattatccc ctttgattgg aacagtatgg atataaccac
     7441 ctttacgaac agttttattg ataaaattat taaataattc ttcttttgtt ttaaatagtt
     7501 ttttggggtt agtaccatat aaaatattgc cattattatc cacaacttga tatagcatat
     7561 catccccacg aatcgttctt tttaaacctt cttcattcga ttgtgataac aaagctatgt
     7621 ttttttcatt gatgtatttt tcaattctag gaacttgtcg ctcataatag tttggtggat
     7681 agatatcctt atttaaggaa tgaatataca aatagagagc aaatacataa gtaatcactg
     7741 tggcaactat actggctatt ataataaaaa taaaagtatg tctaaattgg gtaataaggg
     7801 attgattttt catttaataa cctctctctc tttacatttt attccactta tacccaatac
     7861 cccaaactgt ggaaatatat tctttttctg gtgtcacagc agaaaactta gctcgtattt
     7921 tttttactcg ttcaacaaca gttgaggaat caccttcaga atcacactcc catacttttt
     7981 catagatttg ttctcttgag aaaacttgtc ctgcatggag tgctagtaat tcaacaatat
     8041 catactctat ttttgttaaa tcaatattct taccatcaat tttaattgca cgttccttca
     8101 tatataagca taacttccca aaatacaaag tagatctctt gttctcttca ttgatatact
     8161 gtgaacgctt ttccctacgc agattagctt caatccttgc cataagttca cgcagtccaa
     8221 atggctttgt tacataatca tcccctccaa aggtcaaccc tttgattttg tcggtttctg
     8281 attgctttgc acttaaaaat ataattgggc ataaaacatc atctctaatt gcctgacata
     8341 cctcaaatcc attcatttta ggcatcataa tatccagtat aataaggtca ggctcctgtt
     8401 ttgataactc tatacctgcc tgaccatcat aagctatcaa cacttcatgt ccttttgcct
     8461 tcaattcatc ctcaagtagc attactaggt ccatttcatc atcaataatc aatattttac
     8521 tcaatataac accacctttc tcttatggga aggtcagtat taaaacaata ttcgtctact
     8581 aataactttt ccttccctcc cacattttaa accaaataac gcctcctatc aagaatataa
     8641 tcaaacctat tgctactact ataagctcta tactagctgt ttgcatcact ttagagtaaa
     8701 atgcctcagt tgaaatagtt ccagcttctt taataaaata aattcctgga agcataccta
     8761 gttttactgg ccatgtccat ggaacaaatg cccatacttt ttcaccaacg ctagttaatc
     8821 caagtattgc tgccatcaaa attccaccga tactaatacc aatagatgca cccatacccc
     8881 atgcaaaact cacccaaaga tgaatagcta atagtggaag tgtcccaatc accacaagtc
     8941 ctgctgctga caggaataac catattgttg ctccagatgg cactgcaata ctcattccta
     9001 tgcaaagtat caacgtagca ataaatgtgc atatagttag gcaaaacaga agaaaaagaa
     9061 attttcccag atataatcta acacgagaaa tacctacaca taaaaaacca ttaaaatttc
     9121 cagcaagctc ttcctcctgc actataaatc ctgctaaaat acctactcca ataggaatga
     9181 tgaatacagt ccatactgta aaaaatcctt caaaagcaaa ttcttgtgta cttccagatt
     9241 tagttgtgag ataaaaaacc acacataatg aaaaaataat aggcatacaa agagtaagcc
     9301 actggacagc tgtcctcttt gctcgaagcc attctgatga taatagcttt atcatttgtc
     9361 atccctccta ttgaaccaag ctgcagtaag tgctgttaat actataaaca caaccaaaga
     9421 aacaacaatc ccaataggaa tcactgaagt attcaaaagt gggtctccag cttctaatat
     9481 agtaccgtta ggatgtatgt tgatgattgg gcataccatt ctaactgccc aactccaagg
     9541 tactgcaatc caaaatggtt ttgtcgcagc aaggacacca aatatcatac ctaaagctcc
     9601 aactcccata cttaggaaca tacctttcca tgtagctagc catagctgaa gtggaagtat
     9661 agcaagagaa acaacccagc atacaatact tgcccctata atctgtccaa aaggaactgg
     9721 accagctttc aatattaaac cagttataat tgttacgaat attaaaacta acgttgaaat
     9781 aaaactgtag actgccatag ctataacttt attaatccaa agtgtcatag gagacacatt
     9841 gtgtgtacgt aaagcacgat aatttccagc ttttttttcc tggaaagcta ccaaagtagc
     9901 aaacaatgca aatccaatag gcagaaataa aaatgaccac cagttaaaaa ttaatgctaa
     9961 aagactttgc cacgcccaag aagttgtttg agataatgga ttttgcatga gtgttgattg
    10021 cattataaat gcatatgctg caaaagacac tgggaaaaaa acaataagtt tacccataaa
    10081 tgttctttta tactttaaaa gttctgattg taaagcattc atctgttttg tcctccttta
    10141 agaataacat ctgtaaaaaa ttcttctaga ttttcattag ggtcaggcat tccttgaaat
    10201 aatagtcgtc ctccatttat aattcctata ttatctacaa cttgagcaac ctctgataga
    10261 atatggctag aaagaattac agttatacct ttttttggaa aagaagcaat caattcacgc
    10321 aattcttgaa tgccaaaggg gtcaagacca ttggttggtt catctagtat taacaactct
    10381 gggtcactca aaagtgcaat agctattcct aacctttgtt tcatacccat agaaaattgt
    10441 gaagctctct ttttacctgt atttttcaaa tttactattt ccaacacttc ataaatttta
    10501 tctttaggta ttcccaatat ttttgtatgt actaacaaat tttcctctgc tgtaaggttt
    10561 ccatacaatg caggggattc tattaaagaa cctatttttg caagagattc tcgtttccaa
    10621 ggctttctat caaaaataat acgaccattt gttgggcgaa gaagaccaac aagcatttta
    10681 agtgttgtgg actttcctgc tccatttggt cctaaaagtc catatatact atttcgttca
    10741 atttgtagag aaacattgtt aactgccatt tgttctccat atctttttga taagtttttt
    10801 gtttcaagaa ttaaatcttt catattaatt tctcccttct tgtaatacct taaatctgtt
    10861 actaagagaa tactaacatg aaaaaataac aattctataa cacctactaa tttt
//
LOCUS       DBGJFF010000127        10867 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.129, whole genome shotgun sequence.
ACCESSION   DBGJFF010000127 DBGJFF010000000
VERSION     DBGJFF010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10867
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.129"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            240..704
                     /locus_tag="ACOIAF_003129"
     CDS             240..704
                     /locus_tag="ACOIAF_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HGZ2670127.1"
                     /translation="MATMTDIIEKFIKDLMEEDNSIQIQRNELANLFSCAPSQINYVL
                     TTRFTIDRGYYIESKKGGGGYVQIEKIRKSKDGHIRELLNEKIGSQISYKKAKELLEG
                     LKESDLINERELKLILYAIDDKSLCMPIYELKEKVRSNILKNIIIGLFSIEE"
     gene            711..1205
                     /locus_tag="ACOIAF_003130"
     CDS             711..1205
                     /locus_tag="ACOIAF_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HGZ2670128.1"
                     /translation="MLCQKCNKNKASVYYNKIVNGEKTEMYLCSECAKENTEMNFNLD
                     MPFSMMDIFSNLGFQPKKELEEKLVCPKCNTTYSEFKNNGRFGCSECYNAFSSQVNPM
                     LQNIHGHIEHTGKAPKKSFYKISVENEIKELKEDLDRAIKNEEYELAAQFRDKIKYLK
                     GSID"
     gene            1221..2246
                     /locus_tag="ACOIAF_003131"
     CDS             1221..2246
                     /locus_tag="ACOIAF_003131"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0046314 - phosphocreatine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HGZ2670129.1"
                     /translation="MKENIVMKSRVRLARNLNNYPFPNKLDKECAMEIIEKVKNAFIN
                     SNLEQKEEFDFYKIEDLDQSKKMLMVEEHIISPDLAENDKSAVIVKKDKTISIMINEE
                     DHIRIQTICDDLNLEYAYSVANEIDDLLESSLEYAFNTKLGYLTSCPTNTGTGMRASV
                     MMHLPALSQLGYMDELYKISSQIGIAIRGIYGERTEALGNIYQISNQLTLGRTESNII
                     ENVSGLTKDAISKEIKAREILQKKLGIKLEDKIFRSIGTLENSRVMSSAEAMSHLSNI
                     KMGIEMNYIDKLDLRAIEQLMIGIQPAHQSIMYKSDDVENRDINRATYIRETLEKLRG
                     GMNCELQ"
     gene            2233..4680
                     /locus_tag="ACOIAF_003132"
     CDS             2233..4680
                     /locus_tag="ACOIAF_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HGZ2670130.1"
                     /translation="MNFNRFTQRAKKAIDLAFESAKSLGHNIVGSEHILLGLLREEEG
                     IAAKVLSKVGFTEAYLEGKIVDMEGKGEEISEDIVLSPRSKQILELSGMFANKLKTNY
                     IGTEHILLAIIQEGEGIANKILNYAGVNDRTLAQLTIDMMGISDKNQYKAENSYTGNQ
                     NQAESKVLDKYGRNLTLYAKQNKIDPVIGREKEIQRVIQILSRRTKNNPVLIGDPGVG
                     KTAIAEGLATNIALGNVPETLKNKTLYSLEMGSLLAGAKYRGEFEERIKEVVDEVVKN
                     GNIILFIDEMHTIIGAGSTGEGSIDASNILKPALARGEIQVIGATTIDEYRKHVEKDS
                     ALERRFQPVMVDEPTKEDSIKILEGLRDKYEAHHKVKITDDAIKAAVELSTRYISDRY
                     LPDKAIDLIDEAASKVRLKENTPPSEIKKLELEIENIDKEKEEAVRCQDFEKAAKIRD
                     EQGLLKKQLEDVRERWNKSSKHSDLVDGEVIAEVVGLWTGIPVNKILEEEADRLLRLE
                     EILHNRVIGQEQAVKSISKAIRRSRAGLKDPNRPIGSFLFLGPTGVGKTELSKALAEV
                     QFGDENQIIRIDMSEYMEKHAVSRMIGSPPGYVGHDEGGQLTEKVRRNPYSVILFDEI
                     EKAHPDVFNILLQILDDGRLTDSKGRTVDFKNTIVIMTSNVGASTIGRQKTLGFSIAK
                     GDEEEKSQYEKMKENIMGELKQRFRPEFLNRIDDIIVFHSLNENHISKIVLLMAAKLQ
                     ERLKEMDIKLEMSDEAVKLISKSGFDLEYGARPLKRALQKELEDELSEAILKGNVKKG
                     SNVVAKVKDEKIVFETK"
     gene            4860..6233
                     /gene="radA"
                     /locus_tag="ACOIAF_003133"
     CDS             4860..6233
                     /gene="radA"
                     /locus_tag="ACOIAF_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HGZ2670131.1"
                     /translation="MAKIKTKYVCQSCGYETAKWLGKCPECTKWNTFVEEIDQKSTKK
                     EVFIIDKSSSKPVSINSIESKEEERFTTDINELDRVLGGGIVKGSLVLVGGDPGIGKS
                     TLLIQVSSNVANLGKTVLYITGEESESQIKMRAKRLGINSENLYIFAENNLSIIESYL
                     ESVNPELIILDSIQTVFSPEISSAPGTVSQIKEGTSKFMKISKKMGISTFIVGHVTKE
                     GSLAGPKLLEHMVDTVLYFEGERYNTYRLVRAVKNRFGSTNELGVFEMRDLGLVELDN
                     PSKILISEKPKDVAGSVIISTVEGTRPMLLELQALVSPTSFGIPKRTSTGVDYNRVGM
                     LLAVLEKRVGLQIQNQDVYINIVGGIKINEPSIDLGIAISVASSFRNIPIDEDIAVTG
                     EVGLTGEVRAVSFIEKRIAECKKLGFKKIVVPRSNYDVVKETKGIEIWPVDNLRQAIN
                     IVLGRNQ"
     gene            6236..7306
                     /gene="disA"
                     /locus_tag="ACOIAF_003134"
     CDS             6236..7306
                     /gene="disA"
                     /locus_tag="ACOIAF_003134"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning diadenylate cyclase DisA"
                     /protein_id="HGZ2670132.1"
                     /translation="MENFLDNKNMLYALKMISPGTPLRLGLNNVLRAKTGGLIVIATN
                     EDVMKIVDGGFAINAEYSPSYLYELAKMDGAIVLSGDVKKILFANAQLIPDYFIETSE
                     TGTRHRTAERVAKQTGAIVIGISQRRNVITVYRGNEKYVVEDISKIFTKANQAIQTLE
                     KYKTVLDQAVTNLNALEFNDLVTIYDVALVMQKMEMVMRVTSIIEKYVIELGDEGTLV
                     SMQLEELMGTTRIDQKLIFKDYNKENTEIKELMKKVKNLNSEELIELVNMAKLLGYSG
                     FSESMDMPIKTRGYRILSKIHRLPTAIIENLVNYFENFQQILDASIEELDEVEGIGEI
                     RATYIKNGLIKMKQLVLLDRHI"
     gene            7395..8489
                     /locus_tag="ACOIAF_003135"
     CDS             7395..8489
                     /locus_tag="ACOIAF_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HGZ2670133.1"
                     /translation="MIRKVTRILFILLGFTIGITTYLTLMKDFEILTFGKETYGYIAA
                     VVAGIIIAILGYLIEPWVVNKVKEIAKIVDKELSKYPQTDILLGSMGLIVGFVIAYLL
                     SGLVNRIPIVGGILSLLLYLFLGYLGMKVALKSKNDLFNIGKLGRLANPIKDKDKDKE
                     NKKEVKAIPPKVLDTSVIIDGRIADICKTGFIEGKLIIPAFVLEELRHIADSSDDLKR
                     VRGRRGLDILNIIQKELNIEVEISERDFDDIAEVDSKLLKLAQVLNGKVVTNDYNLNK
                     VAQFQGVEVLNINELANAIKPVAIPGEDMVVQVVKEGKEAQQGVAYLDDGTMIVVDGG
                     RKYMNETIKVLVTSVLQTPAGRMIFAKPKN"
     gene            8618..10795
                     /locus_tag="ACOIAF_003136"
     CDS             8618..10795
                     /locus_tag="ACOIAF_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670134.1"
                     /translation="MRKKIISFLATFIIILTSASQYSFADDISTRGKVIFIDMNRTSM
                     SNMLRIKSLREELDNRGYIGLMNIRGDKGSDDRRSYASMGAGGRANVANEEDINFESS
                     SKDRNIVFESATGKSAKGINNLTINKSINENLNFGEYGSVLGSLGQSLSDNGLKASVL
                     GNSDIIENGQLIKNRNLCLTAMDEYGRIPNGNVDTINKKDLSMPYGISTDYDKLIVET
                     KEAYKNNDVIFVELGDTYRLDLYKPNLNEKTYESMKDNIEKNIDIYLKNIFSMVEEND
                     TIYIASAFPSDLDYKNKRRLSPIIKLNGEGKGILSSSTTRREGIVTNLDVGVEILDNF
                     NIKNQNMVGRKYELINRDDNKEFLMDEYQKIVSISSIRSTILNGFVSIVFLSWIVAMI
                     AILFRKHISKNYKETTFFILKELLKIGIVMPLSFMITPIMNFKTPLAISLGIIIITLT
                     IYLISKVLIKNDLKNMLFFTGLTIVIMVIDAGFGSYLMKSNVMSYDCIIGARYYGVGN
                     EYQGVAIGSAIFTFAILLTYKNIPKWSIIVFSLVILITSASPIMGANVGSAISECVAF
                     LLFILLIYNVKIDFKKIVLLGIAVVFVLGVFVAIDMILGSNSHLGMFVKEIYFNGPGE
                     IIQTFSRKIEMNLKLAQTSAWVNILLTGIGVILVLMINQIRYFKQLMDEYPIVFKGFI
                     ASIAGCLVTLLVNDSGIVSSATAFIYVIVPMITLSINLTALKE"
BASE COUNT         4546 a         1096 c         2008 g         3217 t
ORIGIN      
        1 ggtatattta aaatataaag tatttaaagt tctgaaatct aatcatgaag caaaaaatta
       61 gcatttggtt atcaataatt ataataaata aaaactcata attaatgaaa gaataaattc
      121 atgtctaaat ttctataaaa aatttaaatt tatacaattt agggctttaa aaatttaaat
      181 aataggtgta aaataatagt caaagaaagt caaaaataaa aaggtgtgtg gtgatattaa
      241 tggcaactat gacagatata attgaaaaat ttattaaaga tttgatggaa gaagataata
      301 gcatacaaat tcaaagaaat gaactagcaa acttgtttag ttgtgcacca tcacaaataa
      361 attatgtctt aacaacaaga tttacaatag atagaggata ttacattgaa agtaaaaaag
      421 gtggcggagg atatgttcaa atagaaaaaa tacgaaaaag taaagatggg catattagag
      481 aactactaaa tgaaaaaata ggaagtcaga tatcctataa aaaagcaaaa gaattattag
      541 aaggtttaaa agagtcggat ttgattaacg aaagagaatt aaaattaata ttatatgcaa
      601 tagatgataa atcattgtgt atgccaatat atgaattaaa agagaaggta aggtctaata
      661 ttttaaagaa tataataata ggattattta gtatagagga gtgataattt atgctatgtc
      721 aaaaatgcaa taagaataaa gcatctgtat attataataa gatagtcaat ggagaaaaaa
      781 cagagatgta tttatgtagt gagtgtgcaa aagagaatac agaaatgaat tttaacttag
      841 atatgccttt ttcaatgatg gatatatttt ctaatttagg ttttcaacct aaaaaagaac
      901 ttgaggaaaa gctagtttgt cctaaatgca atacaacata tagtgagttt aagaataatg
      961 ggagatttgg atgtagtgaa tgttataatg catttagtag tcaagtaaat ccaatgcttc
     1021 aaaatataca tgggcatata gaacatacag gaaaagcacc taaaaaatct ttttataaga
     1081 taagtgtaga aaatgagatt aaagaactaa aagaagattt agatagagct ataaaaaatg
     1141 aagagtatga attagcagct caatttagag ataaaattaa atatttaaaa ggttctattg
     1201 actaagattg gagtgatatt atgaaagaga acatagtcat gaaatcaaga gttaggctag
     1261 ctagaaattt aaataactat ccatttccaa ataaattaga taaagaatgt gccatggaaa
     1321 taatagaaaa agtaaaaaat gcgtttatta attctaactt agaacaaaaa gaagaatttg
     1381 atttctataa aattgaagat ttagaccaat ctaaaaaaat gcttatggtt gaagaacata
     1441 taataagccc agatttagca gaaaatgata aaagtgcagt tatagtaaaa aaagataaaa
     1501 ctataagtat aatgataaat gaagaagacc atataaggat acaaactata tgtgatgatt
     1561 taaacttaga atatgcatat agtgtagcaa atgaaataga tgatttgtta gaatcaagtt
     1621 tagaatatgc ctttaacaca aagctaggtt atctaacatc ttgtccaact aatacaggaa
     1681 caggaatgag agcttctgta atgatgcatt taccagcatt aagtcaacta ggctatatgg
     1741 atgaacttta taaaatttca tcacaaatag gtatagcaat tagagggata tatggagaac
     1801 gaacagaagc attgggaaat atatatcaaa tatctaatca acttacactt ggaagaacag
     1861 agtctaatat tatagaaaat gtaagtggac ttacaaaaga tgcaatttca aaagaaataa
     1921 aagctagaga aatattgcaa aaaaaattag gtataaaact agaagataaa atatttagat
     1981 cgatagggac acttgaaaat tcaagagtaa tgagtagcgc agaggcaatg agccatctat
     2041 caaatataaa aatgggaata gaaatgaact atatagataa actagattta agggctatag
     2101 aacaacttat gataggtata caaccagcgc atcagtcaat aatgtataag agtgatgatg
     2161 ttgagaatag agatataaat agagcaacat atattagaga aactttggaa aaattaagag
     2221 gagggatgaa ttgtgaactt caatagattt acacaaagag ctaaaaaggc aattgattta
     2281 gcgtttgaat ctgctaaaag tctaggacac aatattgtcg gaagtgaaca tatactttta
     2341 ggtcttttaa gagaagaaga aggtatagct gctaaagttc taagtaaagt tggatttaca
     2401 gaagcttatt tagaaggcaa aatagttgat atggaaggta agggagaaga aatctcagaa
     2461 gatatagtat taagtccaag aagtaaacaa atacttgaat tatcaggaat gtttgcaaat
     2521 aaattaaaaa caaattatat tgggactgag catatattat tagcaattat tcaagaaggt
     2581 gaaggaatag ctaataagat tttaaattat gctggagtaa atgataggac tttagcccaa
     2641 ttaacaattg atatgatggg tattagtgat aaaaatcaat acaaagcaga aaatagttac
     2701 acgggcaacc agaatcaagc agaatctaaa gtacttgata agtatggaag aaatcttaca
     2761 ctatatgcaa aacagaataa aatagaccct gtaataggta gagaaaaaga aatacaaaga
     2821 gtaatacaaa tattaagtag aagaactaaa aacaatccag tgttgatagg agacccaggt
     2881 gtagggaaaa cagccatagc tgaaggtctg gctacaaata tagccttagg aaatgttcca
     2941 gaaacactaa aaaacaaaac tttatactct ttagagatgg gttcattatt ggctggggct
     3001 aagtatagag gagaatttga agaaagaatt aaagaagttg tagatgaagt tgttaaaaat
     3061 ggaaacataa tcttatttat agatgagatg cacacaataa taggggctgg gtctactgga
     3121 gaaggctcta tagatgcttc aaatatatta aagccagcct tagcaagagg agaaatacaa
     3181 gttataggtg caactacaat tgatgaatat agaaagcatg ttgaaaaaga ttctgctcta
     3241 gaaagaagat tccaaccagt tatggtagat gaaccaacta aagaggactc aataaaaata
     3301 ttagaaggat taagagataa atatgaagct catcataaag ttaaaataac tgatgatgct
     3361 ataaaggcag ctgtagaatt atcaactaga tatatatcag acagatatct gccagataaa
     3421 gctatagact tgattgatga agctgcatca aaagtaagat taaaagaaaa tacacctcct
     3481 tcagaaataa aaaaattaga actagaaata gaaaatatag ataaagaaaa agaagaagca
     3541 gtaagatgtc aagactttga gaaagcagcc aaaataagag atgaacaagg tctacttaaa
     3601 aaacaattag aagatgttag agaaagatgg aacaagtcat ctaaacattc agatttagtt
     3661 gatggagaag ttattgctga agtagtgggc ttatggacag gaataccagt taataaaatt
     3721 cttgaagaag aggctgatag acttttgaga cttgaggaaa tactgcacaa tagagttata
     3781 ggtcaagaac aagcagtaaa atctatttca aaggcaatca gaaggtcaag agcaggtctt
     3841 aaagacccta atagaccaat tggttcattt ttatttttag gtccaacagg agtaggtaaa
     3901 acagaattat ctaaggcatt agcagaagtg caatttggag atgaaaatca aataattaga
     3961 atagatatgt ctgaatatat ggaaaaacat gctgtgtcaa gaatgatagg ttcacctcca
     4021 ggttatgtag gtcatgatga aggtggtcaa ttaactgaaa aagtaagaag aaatccatat
     4081 tcagttatat tatttgatga gatagaaaaa gctcatccag atgtatttaa tattctgtta
     4141 caaattctag atgatggtag actaactgat tcaaaaggaa gaactgtaga ttttaagaat
     4201 actattgtaa taatgacatc aaatgttgga gcatctacaa ttggtagaca aaaaacttta
     4261 ggatttagta tagctaaagg agacgaagaa gaaaaatctc aatatgaaaa aatgaaagaa
     4321 aatataatgg gtgaattaaa acaaagattc agaccagagt ttttaaacag aatagatgat
     4381 ataattgttt tccactcact aaatgaaaat catatatcaa aaatagtact tttaatggca
     4441 gcgaaactac aagagagatt aaaagaaatg gatataaaat tagaaatgag tgatgaggct
     4501 gttaaattaa tttctaaatc tggatttgac ttagaatatg gagcaagacc tcttaaaaga
     4561 gctttacaaa aagagttgga agatgagcta tctgaagcaa tcttaaaagg taatgtgaaa
     4621 aagggaagta atgtagtagc aaaagtcaaa gatgaaaaaa tagtttttga aactaaataa
     4681 tttaatagag ttgcatttta aaatattgca aatataagtt ttataatcta aacaaaattt
     4741 taagggcaat gaatctataa taattcattg tccttaatta ttttatttaa tctttacata
     4801 ttgtctttca gatatgctaa aattatagta tagaataaaa aattaaggat gataaaagta
     4861 tggcaaaaat aaaaacaaaa tatgtatgtc aatcttgcgg atatgaaaca gctaaatggc
     4921 ttggtaaatg tccagaatgt acaaaatgga atacatttgt agaagaaata gaccaaaaaa
     4981 gcaccaaaaa agaggtcttt ataatagata aatcttcatc taaaccagtc agtataaact
     5041 caatagaaag taaagaagaa gagcggttta caacagatat aaatgagtta gatagagtgc
     5101 ttggaggagg tatagttaaa ggctctttag tgcttgttgg tggtgaccct ggtataggta
     5161 aatcaacact tttaatccaa gtatctagta atgttgccaa tttaggaaaa actgttttat
     5221 atataactgg agaagaatca gaatctcaaa taaaaatgag agctaaaaga ttaggtatta
     5281 actcagaaaa tttatatata tttgctgaaa ataatttaag tataattgaa tcttatttag
     5341 aaagtgtaaa tccagaatta ataattctag actcaattca aactgtcttc agtccagaaa
     5401 tatcctcggc accaggaact gtcagccaaa ttaaagaagg tacttcaaaa ttcatgaaaa
     5461 tttctaaaaa aatgggaatt tcaacgttca tagttgggca tgttacaaaa gaaggttcac
     5521 tagcaggacc aaaattatta gagcatatgg ttgatactgt tctatatttt gaaggagaaa
     5581 gatataatac ttatagatta gtaagagctg ttaaaaatcg atttggttca actaatgaac
     5641 ttggagtatt tgaaatgagg gatttaggat tagtagaact tgataatcca tctaaaattt
     5701 taatctctga aaaaccaaaa gatgtagcag gttcagtaat aatatctact gtagaaggta
     5761 cgagaccaat gttacttgaa ttgcaagcat tagtttctcc tacaagtttt ggtataccaa
     5821 aaagaacatc tacaggagtc gattacaata gagtaggtat gttattagca gtattagaaa
     5881 aacgtgttgg tttgcaaata caaaatcaag atgtctatat aaacatagtt ggaggaataa
     5941 agataaatga accctcaatt gatttaggga tagccatttc tgtagcatct agttttagaa
     6001 atataccgat agatgaagat atagctgtta caggagaagt gggacttaca ggagaagtta
     6061 gagcagtaag ctttatagaa aagagaatag ctgagtgtaa aaaacttgga tttaaaaaga
     6121 tagtagttcc tagaagtaat tatgatgttg taaaagagac caaaggcata gaaatatggc
     6181 cagtagataa cttaagacaa gctataaata tagtacttgg gaggaatcaa taaatatgga
     6241 gaattttcta gataataaaa atatgctata tgcattaaaa atgatatctc ctggaactcc
     6301 acttagatta ggtctaaaca atgtactaag agctaagact ggtggattaa ttgtaattgc
     6361 aacaaacgaa gatgtaatga aaatagtaga tggaggattt gctataaatg cagaatattc
     6421 accatcatat ctatatgaat tagctaaaat ggatggagct atagttttaa gtggtgatgt
     6481 aaagaaaata ttatttgcta atgcacaact tatacctgac tattttatag aaacatcaga
     6541 gacaggaaca agacatagaa cagcagaaag agtagcaaaa caaactggtg ctatagtcat
     6601 aggaatttca caaagaagaa atgttataac agtttataga ggaaatgaga agtatgtagt
     6661 cgaagatata tctaagatat ttactaaggc aaatcaggct atacaaactc tggaaaaata
     6721 taagacagta ttggaccaag ctgtaacaaa tttaaatgcc ttagagttta atgatttggt
     6781 aactatttat gatgttgcat tagtcatgca aaagatggaa atggtaatga gagttacaag
     6841 tataattgaa aaatatgtga tagaattggg tgatgaagga actttagtaa gtatgcaatt
     6901 agaagaatta atgggtacaa ccagaataga ccagaaatta atattcaaag attataataa
     6961 agaaaacaca gaaataaaag aacttatgaa aaaggtcaaa aatttaaatt cagaagaact
     7021 aatagaattg gtcaatatgg caaaactatt agggtatagt ggtttttcag aaagtatgga
     7081 tatgcctata aaaacaagag gttataggat tcttagcaaa atacatagac taccaacagc
     7141 aataatagaa aacttagtaa attattttga aaactttcaa caaattttag atgcatctat
     7201 tgaagaatta gatgaggttg aaggaatagg tgaaataaga gcaacatata taaaaaatgg
     7261 actcataaaa atgaaacaat tagtcttatt agatagacac atatgatgaa ttttaaatga
     7321 attttactac ttttttcttg aatataatat acactttggt ctataataga taagtataac
     7381 tagggggtga agcattgata aggaaagtaa caagaatatt gtttatttta cttggattta
     7441 caataggtat aacaacctat ttaacattaa tgaaagattt tgaaatatta acatttggaa
     7501 aagaaacata tggttatatc gcagctgtag tagcaggtat aataatagcc attttaggat
     7561 atcttataga accatgggtt gtaaataaag taaaagaaat tgctaaaata gtggataaag
     7621 aattgtcaaa atatcctcag actgatatat tgttagggtc tatgggtctt atagtaggat
     7681 ttgttattgc gtatttatta agtggattag taaatagaat accaatagta ggtggaatac
     7741 tatccttact attatattta ttcctaggat atcttggaat gaaagttgcg cttaaaagta
     7801 aaaatgattt atttaatatt ggaaaactag gaagattagc aaatcctatt aaggataaag
     7861 ataaggataa agaaaataaa aaagaagtaa aagccattcc tccaaaagta ttagatacaa
     7921 gtgtaataat tgatggaaga atagctgata tttgtaagac tgggtttata gaaggaaaac
     7981 ttattattcc tgcttttgtt ttggaggaac taagacatat tgcagattct tcagatgatt
     8041 taaaaagagt aagaggtaga agaggtctgg atatattaaa tataattcag aaagaattaa
     8101 atatagaagt tgaaataagt gagagagact ttgatgatat agcagaagtt gatagtaaat
     8161 tattaaagct tgctcaggtt ttaaatggaa aagttgttac aaatgattat aacttaaata
     8221 aagttgctca attccaaggt gttgaagtat taaatataaa tgagttagct aatgctataa
     8281 aaccagttgc tataccaggt gaagatatgg ttgttcaagt ggttaaagaa ggaaaagagg
     8341 ctcaacaagg tgttgcatat ctagatgatg gaactatgat agttgttgat ggaggaagaa
     8401 aatatatgaa tgagactata aaagtactcg ttacttctgt tcttcaaact cctgcaggta
     8461 gaatgatatt tgcaaaacct aaaaattaat atgttcaaag aaacctataa tttgggctgt
     8521 ttattttaaa tagatggtcc aaatttattt atttataacg ataatgttta atgtattctt
     8581 aaagatgggg atttaaatca gaaagcaggg agaatttatg agaaaaaaga ttatatcttt
     8641 cttagcaaca tttatcataa tattaacatc agcaagtcag tatagttttg cagatgatat
     8701 atcaacacgt ggaaaagtaa tatttataga tatgaataga actagtatga gtaatatgtt
     8761 aagaataaaa tcattaagag aagaactaga caatagaggg tatattggct tgatgaatat
     8821 aagaggcgat aaaggaagtg atgatagaag gtcatatgct agtatgggtg ctggtggtag
     8881 agctaatgtg gctaatgaag aagatattaa ttttgaaagc tcgagtaagg atagaaatat
     8941 agtttttgaa tcagctacag gaaaaagtgc aaaaggtata aataatttaa ctataaataa
     9001 gtctataaat gaaaatttaa actttggaga gtatggttct gtattagggt cattaggaca
     9061 aagtttatct gataatggat taaaagcatc tgtattgggt aattcagata taattgaaaa
     9121 tggtcaactt ataaaaaata gaaacttatg tctaactgca atggatgaat atggaagaat
     9181 acctaatgga aatgtagata ctataaataa aaaagactta tctatgcctt atggaatatc
     9241 aactgattat gataaattga ttgtagagac taaagaagct tacaaaaata atgatgtgat
     9301 ttttgtggag cttggagaca catatagatt agatttatat aaaccaaatt taaatgaaaa
     9361 aacatatgag agtatgaaag ataacataga aaaaaatata gatatatatt taaaaaatat
     9421 attttctatg gttgaagaaa atgacactat ctacatagca agtgcatttc caagtgattt
     9481 agattataaa aataaaagaa gactttctcc aataataaaa ttaaatggag aagggaaagg
     9541 tatattatca tcttcaacta ctagaagaga aggtattgta acaaatttag atgttggggt
     9601 tgaaatatta gacaacttta atataaaaaa tcaaaatatg gttggaagaa aatatgaact
     9661 tattaacaga gatgataata aagaattttt gatggatgaa tatcaaaaga tagtatctat
     9721 ttcaagcata aggtctacaa tactgaatgg ttttgtaagt atagttttct tgtcatggat
     9781 agttgctatg attgcaattt tatttagaaa gcatatatca aaaaattata aagaaacaac
     9841 attttttata ttaaaggaac ttttaaaaat aggaatagta atgccactgt catttatgat
     9901 aacaccaata atgaatttca aaacaccatt agcaatatct ttaggaataa ttataataac
     9961 acttactata tatcttataa gtaaagtttt aataaagaac gatttaaaaa acatgctgtt
    10021 ttttactgga ttaaccatag taattatggt tattgatgct gggtttggct catatttaat
    10081 gaaaagcaat gttatgagtt atgattgtat aattggagct agatattatg gagtaggaaa
    10141 tgagtatcaa ggtgtggcaa tcggtagtgc aatatttaca tttgcaattt tacttacata
    10201 taaaaatata ccaaagtggt ctataatagt tttttctttg gttatcttaa ttacaagtgc
    10261 atctccaatt atgggagcaa atgttggaag tgctatttct gaatgtgttg catttttact
    10321 atttatacta ttgatatata atgtaaagat agatttcaaa aaaatcgtat tattaggtat
    10381 agcagtagta tttgtattag gagtttttgt agcaatagat atgattttgg gaagtaattc
    10441 acatttaggt atgtttgtaa aagaaattta ctttaatgga ccaggagaaa taatacaaac
    10501 atttagtaga aagatagaga tgaatttaaa gctagctcaa accagtgcat gggtaaatat
    10561 actgttaact ggaataggtg taattttagt tcttatgatt aatcagatta gatactttaa
    10621 gcaactgatg gatgagtatc caattgtatt taaaggattt atagcatcaa tagcaggatg
    10681 tttagtaact cttttagtga atgactctgg gattgtgtca tcagctacag cctttattta
    10741 tgtaattgta cctatgataa cattaagtat aaatcttaca gcattaaaag aataagtact
    10801 gtatgaataa tatgtaaata ttaatacatt aatttaactt ctttatttac aaaataaaga
    10861 agaaagt
//
LOCUS       DBGJFF010000128        10813 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.130, whole genome shotgun sequence.
ACCESSION   DBGJFF010000128 DBGJFF010000000
VERSION     DBGJFF010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10813
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.130"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(178..1263)
                     /locus_tag="ACOIAF_003137"
     CDS             complement(178..1263)
                     /locus_tag="ACOIAF_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670135.1"
                     /translation="MQVQEKILELLYTINEDVVSIKNDMSEVKQEINVLKEDMSEVKQ
                     EMTVMKEDMSEVKQEINVLKEDMSEVKQEMTVMKEDISEVKQEMTVMKGDMSEVKQEI
                     NVLKGDMSEVKQEMTVMKEDMSEVKQEMTVMKEDMSEVRQEINIMKEDISEVRQEMTV
                     MKEDMSEVKQEINVLKEDMSEVRQEINIMKEDMSEVRQEMTVMKEDTSEVKQEINVMK
                     KDMSEVRQEITVMKEDVSEVKSEVNFMQNKINNINEDMNGIKDEVSIANEKLYGIEIK
                     IDSLECEDKSMKEIQIEQNNILGSLLHNSEINKATHDNIEHNIAYIKGDTNSIKEDIA
                     EIRRDLNLVELATSKNWSDIVKLKSVK"
     gene            complement(1499..2584)
                     /locus_tag="ACOIAF_003138"
     CDS             complement(1499..2584)
                     /locus_tag="ACOIAF_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HGZ2670136.1"
                     /translation="MYNFLGKELKSPLIIGSGPLTYSAAGCKILSDAGAGAVVTKTIR
                     KERAINPAPHMVRNTTNALLNNEKWTDFEPEQWIDFEIPQMKRDGTVCIASVGHTIEE
                     SSELVEKVANAGADFIELVSYDYRDLIPMLKDAKERVNIPVIVKLPPMIDEIGDFAKK
                     LEEAGADAITACDSVGPAFRIDIETGQPLLGGNGIGYLSGETIKPITLQRIYEIKKQV
                     NIPIIGLGGCVSGDDALEMIMAGADFVGICSVVILKGAQVISKIHDDLKSNLNRLGYN
                     TIENACGIVHNHMGDVNERLSFEFTYDEEKCTKCKMCERVCAYQARKLNDKMELNEEE
                     CRYCGLCISVCKPKALGRKISCSELNV"
     gene            complement(2620..3933)
                     /locus_tag="ACOIAF_003139"
     CDS             complement(2620..3933)
                     /locus_tag="ACOIAF_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HGZ2670137.1"
                     /translation="MIAIKSKYLISSADEIYTNSAVVVENDIIKDILPNEEVEIKYKN
                     IEEIIDKSDAIIMPGFVNGHMHQYGVLSRGIPANVHFTDFEGFLNDYWWPFIENRIGL
                     KEVKATTKASAIELIESGVVAFCDTLEAPNTEEGTLIEQGKILEEIGMKAILSLESCE
                     RISFENGLRCLDENSNLIKWSRENSKLINGIMCTHTSFTCSDRFIKKAKEDAKKLNAP
                     MQFHLCESIYEPNYAEKHFGKKAVDYYNDLDILDKTVLASQCVKVNDEEIDILKEKGV
                     KVVHMPLSNCEVGGGFSPVPKMIKKGIKVALGTDGYINDFFEVMRGAFLMHKSVEEDA
                     SVMPANLVFRMATEHGAHVLGLQNSGKIAVGNKADIIVMEDEFKTPVTLDNIFDQIVV
                     QGKKEFISNVYIDGRHILKEKQLVDLDKKAIVKEMKEVACEFWKF"
     gene            complement(3934..6225)
                     /locus_tag="ACOIAF_003140"
     CDS             complement(3934..6225)
                     /locus_tag="ACOIAF_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HGZ2670138.1"
                     /translation="MVNKEVRKIDSEAIVTGKPIYTEDLIFHKDVLTIKLLRSPHAFA
                     KIKNIDIKNALKVKGVVNIFTYKDVPNYRYSMVGESYPEASPHDQLILEDVVRYVGDE
                     VAIIAAEDERAAIKAMKLIKVEYEVMTPILDARESEGNKILIHEEDDLFCPFDFGLDA
                     TKNIVSREKMGKGDVEKEFKESDVIVEHSYMTQSQAHCMMETHRAYTYIDTNGRLVVT
                     SANQSAYHMRRQVSKALGIPLSKVRVIKPRIGGGFGGKNIAVTEIYASFVTWMTKRPA
                     KLIYTREETFAMTNTRHQMFFDIKIGADKEGNIKAIDMKALNNTGAYGDNGPSVCSES
                     GHNVLPTYNNVPAIKFDGKTVYTNLVPGGALRGYGATQGTFALDCAIDELAHKLNMDP
                     TEIKLKNIIKKQTEGGIMNFPIRSCALDECIIRGKKLIGWDEKYPVRDIGNNKVRAVG
                     MSVGTHGSGIANIDMAIVNMRMDEDGTYKLFSGSSDLGTGSDTILAQIAAKALNTSID
                     RISVYTGDTDMCPYDTGAYASCTTYVTGNATIKSAESLKQKILKVAQKKLDLPIDDLI
                     LYEDRVCYSEDVEKFVLLSELGKESVGGGNQEVLYSSESYGIQESAKPFLAGFVEIEL
                     DKTTGEFKVINYACSTDCGTVMNPALARIQVEGGVTQGIGLAMYEDPRHGSDGKLQTS
                     NLLQYKVPTKKDIGNIIVDFVDNYEPTGPYGAKSLGEIVIHTPAPAIANAIYNATGVR
                     IRELPITSEKVYMAMKKLNQGGK"
     gene            complement(6215..7009)
                     /locus_tag="ACOIAF_003141"
     CDS             complement(6215..7009)
                     /locus_tag="ACOIAF_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HGZ2670139.1"
                     /translation="MITIKEYVVPKSLDEAYELLISRKNNIILGGCGFLKLGSKNIGS
                     AIDLKDLALDYINETDDSILIGADTSLRTLELNKVIKNYCNGVISNAVSNIVGVQFRS
                     GARVGASVFAKYGFSDLIPSLLVVDAKVKLYKKGVMNLADFLENELEKDILIEVILPK
                     KDAIGVFDSIRKCTGDFAVLNGAMLKEGSIYKIAIGARPRRAKIAYKASEILSLENDI
                     EKAGEVSSEELSFGSNIRGSKEYRKDMAKALVVRMYNSIGGECDGK"
     gene            complement(7048..7494)
                     /locus_tag="ACOIAF_003142"
     CDS             complement(7048..7494)
                     /locus_tag="ACOIAF_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HGZ2670140.1"
                     /translation="MIIKCTINNKEKELSIRPDDFLADTLRENGYVSVKKGCDTGSCG
                     LCTVMVNDKAVLSCNYLTARVEGQHITTLEGVLDEAKKFGDFLADEGADQCGFCAPGF
                     IMMVLAMKRELQNPTEEEVIDYVNGNLCRCTGYKGQLRAIHKYLNT"
     gene            complement(7566..8972)
                     /locus_tag="ACOIAF_003143"
     CDS             complement(7566..8972)
                     /locus_tag="ACOIAF_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HGZ2670141.1"
                     /translation="MMDILIKNAIIVTVNKEREVIFDGALVVKDNKIADIGNSKEIES
                     KYTDVKKIIDAKGKVLFPGFINTHNHLFQTLLKGLGDDMVLKDWLETMTFPAANYLEP
                     KDTYDAAMLGCIEGLRSGITTMVDYMYPHSKPGLCDGIIDAYKELGIRGILGRGCMNT
                     GAQFGVHPGIMQDVETVEKDVRRLFEKHHNTENGRIKIGVAPAAIWSNSQEMLEMLWR
                     VVKEYDDALFTVHISETPFDREAAKELHGQYDIDVLEKLGILGPNVLMVHCVYLTEKD
                     MELTKKYDMKVSHNTASNMYLSSGVAPVPEMLKKGITVSLGVDGAASNNSQDMLELMK
                     LTALQHKVNKCDPLAMSAEKVLELATIDGARAIGMEDEIGSLEIGKKADLLIFNPMLS
                     PKAIPMHNPVSTLVYSSSMKNIESVIVDGNIIMEDSKILTANEEKALKDAQDTAERLC
                     VRGTIKNRMEGHKWNSLY"
     gene            complement(8990..10360)
                     /gene="hydA"
                     /locus_tag="ACOIAF_003144"
     CDS             complement(8990..10360)
                     /gene="hydA"
                     /locus_tag="ACOIAF_003144"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HGZ2670142.1"
                     /translation="MGTILRGGTIITSDKTYISDLRIEDEKIVEIGNNLEINGDKVID
                     ATGKIVIPGGIDTHTHFDMNAGSITTADNFETGTRAAIAGGTTTILDFAEANEGENLL
                     QGVEAYHKKASGNCYCDYGFHMTITCLNKDTFNHMEKLISDGIVSFKMYMAYDGMKVD
                     DGTIYRVLKKARDLGCIVGFHCENGDLLDVLIEENIQNGNLEPKYHPLTRPNIVEKES
                     VSRLADITKLSNSKSYVVHLSCRESLETVKMAREKNIDMIVETCPQYLLLEDNLYNKD
                     KFEGAKYVMSPPLRKKEDINYLWEGLSSGDIQTVGTDHCSFNFKGQKDLGIDDFSKIP
                     NGAPGVEHRLALLYTYGVLENRISANKFVEVTSTNAAKIFGMYPKKGEIAVGSDADIV
                     ILNINKEETISYKTQKQNVDYTPYEGFKVKCKVEDVFLRGNHVVQSCNVKEHPTGQYI
                     KRKITK"
     gene            complement(10493..>10813)
                     /locus_tag="ACOIAF_003145"
     CDS             complement(10493..>10813)
                     /locus_tag="ACOIAF_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HGZ2670143.1"
                     /translation="VEKADCYTGNTIESERFFPGWLYDEEDEFVQAAYKGLKEAGIDS
                     EITQYSFCTNGSHYAGEAGIKTIGFGPSKENLAHTIDEYIEQEQLFIGTEGYYGILKS
                     VYGK"
BASE COUNT         3139 a         2196 c         1121 g         4357 t
ORIGIN      
        1 ttatttttat aaattaattg tatttacata aaaaaaaaga cctaaaaaca aatttttaac
       61 ggtcttttct caatttttta ttttcatttc taaaatttat aataaatagt aagaacacta
      121 taaattcttt tatataaaat tcttagtttg tggatgataa attagtgaat taaaatacta
      181 ctttactgat tttagtttga caatatcact ccaattttta ctagtggcta actctactaa
      241 atttaagtct cttcttattt cagctatatc ctcttttata gaattagtgt caccttttat
      301 atatgctatg ttatgttcta tattatcatg agtagcttta tttatttcag aattatgtaa
      361 taaacttcct aaaatattat tctgttctat ctgtatctct ttcattgatt tatcttcaca
      421 ttctagacta tcaatcttaa tttcaattcc atataatttt tcatttgcta tggatacctc
      481 gtccttaatt ccattcatat cttcattaat gttatttatt ttattttgca taaagtttac
      541 ttctgattta acttcggaaa catcttcttt cattacagtt atttcttgtc ttacttctga
      601 catgtctttt ttcattacat ttatctcttg ttttacctct gatgtatctt ctttcattac
      661 agtcatttct tgtcttactt cagacatatc ttctttcatt atatttatct cttgtcttac
      721 ttcggacata tcttctttta atacatttat ctcctgtttt acttcagaca tatcttcttt
      781 cattacagtc atttcttgtc ttacttcgga tatatcctct ttcattatat ttatctcttg
      841 tcttacctct gacatatctt ctttcattac agtcatttct tgttttactt ccgacatatc
      901 ttctttcatt acagtcattt cctgttttac ttccgacata tcccctttta atacatttat
      961 ttcctgtttt acttccgaca tatctccttt cattacagtc atttcttgtt ttacttctga
     1021 tatatcttct ttcattacag tcatttcttg ttttacttcc gacatatctt cttttaatac
     1081 atttatttcc tgttttactt ccgacatatc ttctttcatt acagtcattt cttgttttac
     1141 ttccgacata tcttctttta acacatttat ctcttgtttt acttccgaca tatcattttt
     1201 tatgctaact acatcttcat ttatagtata taataactct aaaatcttct cttgcacttg
     1261 catcaccatc cctattaatt attataacat tttacacaaa tgctatacaa tgttaacata
     1321 tatatcagaa ttattaaaat tttaattaaa caaaattgat tcactttatt acttcaaaac
     1381 taattaatat ctaaaataac tacaaataaa caaaaataag gtcaacaaca aaaaaggctt
     1441 atctctttga gatagccttt cttattttta tttaaaactt tgttttttat aaaataatct
     1501 atacattcag ctctgaacat gaaatttttc ttccaagtgc ttttggttta caaactgata
     1561 tacaaagtcc acaatatcta cactcttctt cattcaattc catcttatca ttcaattttc
     1621 tagcttgata tgcacaaact ctctcgcaca tcttacattt agtacatttt tcttcatcat
     1681 aagtaaactc aaagctaagt ctttcattaa catctcccat atgattatgt actataccac
     1741 aagcattttc tatagtatta tatcctaatc tattaagatt agattttaaa tcatcatgta
     1801 ttttagatat tacttgtgcg ccttttaaaa ttactactga acatatacca acaaagtcag
     1861 cacctgccat aatcatttct aaggcatcat ctccacttac acatccacct aagccaatta
     1921 ttggtatatt aacttgtttt tttatttcat atattctctg taaagttatt ggcttgatag
     1981 tttctccact taaatatcct attccatttc cacctaataa aggttgccct gtttctatgt
     2041 ctattctaaa tgctggtcct acagaatcac aagcagttat tgcatctgct cctgcttctt
     2101 ctaatttttt agcaaaatcg cctatctcat ctatcatagg aggaagttta actattacag
     2161 gtatatttac tctttccttt gcatctttaa gcattggtat caaatctcta tagtcataag
     2221 atactaattc aataaagtca gctcctgcat ttgcaacttt ctctactaat tcgctacttt
     2281 cttcaattgt atgacctact gaagctatac agacagtccc atctcttttc atttgtggta
     2341 tttcaaaatc tatccattgt tctggctcaa aatctgtcca tttctcatta tttaaaagag
     2401 cattagttgt atttctaacc atatgtggtg ctggatttat agctctttct tttcttatag
     2461 tcttagtcac aactgcccct gctcctgcat cacttaatat cttgcatcca gctgcactat
     2521 atgttaacgg tcctgaacct attataagtg gtgattttaa ttctttacct aaaaaattat
     2581 acattatctg cttccccttt tcatatatat ttacattcgt taaaatttcc aaaactcaca
     2641 agcaacttct ttcatttctt taacaatagc ttttttatcc aaatcaacta actgtttctc
     2701 ttttaaaata tgtctaccat ctatgtatac atttgatata aattcttttt taccctgaac
     2761 tactatctgg tcaaatatat tatcaagagt cacaggagtt ttaaattcat cttccataac
     2821 tattatatca gccttattac caactgctat tttaccagaa ttttgaagtc ctaatacatg
     2881 agcaccatgc tctgttgcca ttctaaatac taaatttgct ggcattactg atgcatcttc
     2941 ttcaactgat ttatgcatta aaaatgcacc tctcataact tcaaaaaaat catttatata
     3001 tccgtcagta cccagtgcca cttttatgcc ttttttaatc atttttggta ctggagaaaa
     3061 acctccacct acttcacagt tactaagagg catatgaacc acttttacgc ctttttcttt
     3121 taatatatct atttcttcat catttacttt tacacactga gatgctaata cagttttatc
     3181 caatatatct aaatcattat agtagtcaac tgcttttttg ccaaagtgtt tttcagcata
     3241 gtttggctca tatatactct cacataaatg aaattgcatt ggtgcattta atttttttgc
     3301 atcttcttta gctttcttaa taaatctatc agaacatgtg aagcttgtat gtgtacacat
     3361 tattccattt attaatttac tattttctct actccacttt attaaattag aattttcatc
     3421 taaacatcta agtccatttt caaagcttat tctttcacaa ctttctaagg ataaaatcgc
     3481 tttcatgcct atttcttcta gtatttttcc ttgctcaatt aaagttcctt cttctgtatt
     3541 tggagcttct agggtatcac aaaaagctac cacaccagat tctataagtt ctattgctga
     3601 tgctttagtt gttgccttca cttcttttaa tccaattcta ttttctataa atggccacca
     3661 atagtcattt aaaaaacctt caaagtcagt aaaatgtaca tttgccggta ttcccctaga
     3721 aagaactcca tattgatgca tatgaccatt gacgaatcct ggcattatta tagcatctga
     3781 tttatctatt atttcttcaa tatttttata ttttatttca acttcttcat ttggcaatat
     3841 atcttttata atgtcatttt ctactacaac agcagaattt gtatatattt catcagcaga
     3901 agatattaag tatttacttt taatagcaat catttattta cctccctgat ttaatttttt
     3961 catggccata taaactttct cagaagttat aggaagttct cttatacgaa ctcctgtggc
     4021 attgtatatt gcatttgcta ttgctggtgc aggtgtatga ataactatct ctcctaaaga
     4081 tttagctcca tatggacctg ttggctcata attatctaca aaatcaacaa ttatgtttcc
     4141 tatatctttt tttgttggaa ctttgtattg taatagatta gaagtttgaa gctttccatc
     4201 agaaccatgt ctagggtctt catacattgc aagccctata ccttgagtta caccaccttc
     4261 aacttgtatt ctagcaagtg caggattcat aactgtaccg caatctgtag aacaagcata
     4321 atttattact ttaaactccc ctgttgtttt atctaactct atttcaacaa atcctgctaa
     4381 gaatggttta gcactctctt gtataccata agattcagat gaatataaaa cttcttgatt
     4441 tccaccacca acagattctt ttcctaattc acttaacaaa acaaacttct ctacatcttc
     4501 actataacaa actctgtctt catatagtat taaatcatct attggtaaat cgagtttctt
     4561 ctgtgctact tttaatatct tttgttttaa actttcagca gattttattg tagcatttcc
     4621 agttacataa gtagtacaag aagcgtatgc accagtatca tatggacaca tatctgtatc
     4681 tcctgtataa acggaaattc tatcaataga tgtattcaat gcttttgcag ctatttgtgc
     4741 aagtattgtg tcagaaccag ttcctaagtc tgaagaccca gaaaacaact tataagttcc
     4801 atcttcatcc atcctcatat ttactattgc catgtctatg ttggctatac cagaaccatg
     4861 agttcccaca gacattccaa cagccctcac cttattgttt ccaatatccc taacagggta
     4921 cttttcatcc catccaataa gttttttacc tctaattata cattcatcaa gtgcacaact
     4981 tcttattgga aaattcatta ttccgccttc agtttgtttt tttataatat tctttaactt
     5041 tatttctgtt gggtccatat taagtttatg cgctaattca tcaattgcac aatcaagtgc
     5101 aaatgtacct tgtgttgctc catatcctct taatgctccc cctggaacta aattagtata
     5161 tacagttttc ccatcaaatt ttattgcagg tacattatta tatgttggaa gtacattatg
     5221 acctgattca gagcaaactg aaggtccatt atcgccataa gcgcctgtat tgttcaatgc
     5281 tttcatatct atagctttta tgtttccttc tttatctgcc ccaattttta tatcaaaaaa
     5341 catctgatgt ctagtattag tcattgcaaa agtttcttct ctagtataaa ttagttttgc
     5401 tggtcttttt gtcatccaag ttacaaatga tgcatatatc tctgttacag ctatgttttt
     5461 tccaccaaaa cctccaccta ttcttggttt tattactctc acttttgaaa gaggtattcc
     5521 cagtgcttta gaaacctgtc ttctcatatg ataagctgat tggtttgcag aagttactac
     5581 taatctgcca ttcgtatcta tatacgtata agctctatga gtttccatca tacaatgggc
     5641 ttgtgattga gtcatataag aatgttctac tattacatca ctttctttaa actctttttc
     5701 tacatcccct ttacccattt tttctctcga tacaatattt tttgtagcat ccaatccaaa
     5761 atcaaatgga caaaacaaat catcttcttc atggattaaa attttatttc cttccgattc
     5821 tcttgcatcc aatataggag tcattacttc atattcaacc tttataagtt tcatagcttt
     5881 aatagcagcc ctttcatctt cagcggctat tatagcaact tcatctccta catatctaac
     5941 tacgtcttca agtattaatt ggtcatgagg tgatgcttct ggatatgact ctccaaccat
     6001 tgaatatcta taatttggaa catctttgta agtgaatatg tttactacac cttttacctt
     6061 taatgcattt tttatatcta tgttttttat cttcgcaaaa gcatgtggac ttctaagaag
     6121 cttaattgtt aaaacatctt tatgaaaaat taaatcttct gtatatattg gttttccagt
     6181 aacaatggct tcactatcta tttttcttac ctctttattt accatcacac tcacctccaa
     6241 tactattata cattctcact actaaggctt ttgccatatc ttttctgtat tctttacttc
     6301 ctcttatatt acttccaaat gaaagttctt cacttgatac ttcaccagct ttttctatat
     6361 cattttctaa acttaatatt tcacttgctt tataggcaat ttttgctctt cttggtctag
     6421 cgcctatcgc aattttataa atacttcctt cttttaacat tgctccattt aatactgcaa
     6481 aatcacctgt acattttctt atagaatcaa atacacctat agcatctttt ttaggtaata
     6541 taacttctat taatatatct ttttcaagct cattttctaa aaaatcagct aagttcataa
     6601 cccctttttt atataactta acctttgcat ctaccactag taatgaaggt attaaatctg
     6661 aaaatccata cttagcaaat acgctagctc ctactctagc tccacttcta aattgaactc
     6721 ctactatatt agaaacagcg tttgaaatta ccccattaca gtaattttta ataaccttat
     6781 ttaattctaa agttcttaaa cttgtatctg ctccaattaa tatgctatca tcagtttcat
     6841 ttatataatc taaagctaag tcttttaaat ctattgctga accaatattt ttactaccta
     6901 gttttaaaaa tccacatcct ccaagtatta tgttgttttt tcttgatatt agcagttcat
     6961 aagcttcatc taaacttttt ggaactacat attcctttat agttatcata ctctctcccc
     7021 atatcaactt aattttcact taaaagtcta agtgtttaaa tatttatgta ttgctcttaa
     7081 ttgaccttta tatcctgtac atctacaaag gttaccattt acataatcta taacttcttc
     7141 ttctgttgga ttttgaagct ctcttttcat tgccaaaacc atcattataa atccaggagc
     7201 acaaaatcca cactggtcag ccccttcgtc tgcaagaaaa tctccaaact tctttgcttc
     7261 atccaagaca ccctcaagtg ttgtaatatg ctgaccttct actctagcag taagataatt
     7321 acatgaaagc acagccttgt catttaccat cactgtacat aacccacatg agcctgtatc
     7381 acaccctttt ttaacactta catatccatt ttctcttaat gtatctgcta aaaaatcatc
     7441 tggtcttata cttaattctt tttctttatt atttatggta catttgataa tcaaataaat
     7501 cacctcaaaa ctcatatttt taagctaaag ttttactata cttttttatg aataaattac
     7561 atttattaat aaaggctatt ccatttatga ccttccattc tattttttat agttcctctt
     7621 acacaaagtc tctcagctgt atcttgagca tcttttaatg ctttctcttc attagctgtc
     7681 aatatttttg aatcttccat tattatattt ccatctacta taacactctc tatatttttc
     7741 atactagaag aatatactaa tgtagatacc ggattatgca taggtatcgc ctttggtgaa
     7801 agcattggat taaatattag taagtctgct tttttaccta tctccaatga acctatctca
     7861 tcttccattc caatagctct tgctccatct atagttgcca actcaagaac tttttctgcc
     7921 gacattgcta atgggtcaca cttattaact ttatgttgaa gtgctgttaa tttcataagt
     7981 tcaagcatat cttgagagtt attactagcg gctccatcta ctcctaaact aactgttatt
     8041 ccttttttaa gcatttctgg cactggtgca acacctgaag ataagtacat attacttgct
     8101 gtattatgag acactttcat atcgtatttt ttagtaagtt ccatatcttt ttcagttaga
     8161 tacacacaat gtaccataag cacatttggt ccaagtattc ctaatttttc taaaacatct
     8221 atgtcatatt gcccatgcaa ttcttttgct gcttctctat caaaaggagt ctctgatata
     8281 tgtactgtaa ataaagcatc atcatattct tttactactc tccatagcat ttcaagcatt
     8341 tcttgagaat ttgaccatat tgctgcagga gctactccta ttttgattct tccattttct
     8401 gtattatggt gtttttcaaa caatcttctt acatcttttt caaccgtttc tacatcttgc
     8461 attattccag gatgaactcc aaattgagct cctgtattca tacatcctct tccaagtatt
     8521 cctcttattc ctaattcttt ataagcatct attattccat cacaaagacc tggcttacta
     8581 tgaggataca tgtagtcaac cattgttgtt ataccacttc taagtccttc aatacatcca
     8641 agcattgcag catcataagt atcttttggc tctaaatagt tagctgctgg aaatgtcata
     8701 gtttctaacc agtctttaag taccatatca tctcccaatc cttttaataa agtttggaat
     8761 aaatgattat gtgtatttat aaaacctggg aataaaactt tacctttagc atctataatt
     8821 tttttaacat cagtatattt actttctatc tctttagaat ttccaatatc agcaatcttg
     8881 ttatctttta ctactaatgc cccatcaaaa ataacttctc tctctttatt cacagttact
     8941 attatggcat tcttaataag tatatccatc attcttattt ccccccgcat tatttagtaa
     9001 tttttctttt aatatattga cctgttggat gttctttaac attacaactt tgtactacat
     9061 gatttcctct caaaaataca tcttcaactt tacatttaac cttaaatcct tcatatggag
     9121 tataatctac attttgtttt tgagttttat aactaattgt ttcttcttta ttgatattta
     9181 aaatgactat atctgcatca cttcctacag ctatctcacc ttttttagga tacattccaa
     9241 atatttttgc agcattcgta gaagtaactt ccacaaactt atttgcactt attctatttt
     9301 ctaagactcc atatgtatat aaaagtgcta acctatgttc aacaccaggt gctccatttg
     9361 gtatcttact aaaatcatca attcccaagt ctttttgccc cttaaaatta aatgaacagt
     9421 gatctgtgcc aactgtttga atatctccac tagacaatcc ttcccaaagg taatttatgt
     9481 cttctttttt tcttaaagga ggagacatta catacttagc accctcaaat ttatccttat
     9541 tatataaatt gtcttctaat aatagatact gtgggcatgt ttctactatc atatctatat
     9601 ttttttctct agccattttt acagtttcaa gtgactccct acaacttaag tggactacat
     9661 aggattttga attagataat ttagttatat cagcaagtct tgaaacagac tctttctcaa
     9721 ctatgttagg tcttgtaagt ggatgatatt ttggctccaa attaccattt tgtatatttt
     9781 cttctatcaa tacatctagt aaatccccat tttcacaatg aaatccaact atacaaccta
     9841 aatcccttgc tttctttaaa accctgtata ttgttccatc atctactttc atgccatcat
     9901 atgccatata catcttaaaa gatactatcc catctgaaat aagtttttcc atatgattaa
     9961 atgtatcttt atttaaacat gtaattgtca tatgaaaacc ataatcacaa taacaatttc
    10021 cacttgcttt tttatgatat gcttctacac cttgcagtag attttccccc tcattggctt
    10081 ctgcaaaatc tagtattgta gtagttcctc cagctatagc tgctcttgtt cctgtttcaa
    10141 aattatctgc agttgtaata gaacctgcat tcatatcaaa atgagtatgt gtgtctattc
    10201 caccaggaat tacaatttta cctgtagcat caataacctt atcaccattt atttctaagt
    10261 tatttcctat ttcaactatc ttttcatctt ctatccttaa atcacttata taagtcttat
    10321 cagaggtaat tatagttcca cctcttagta ttgttcccat atatacctcc taacttttaa
    10381 gctttaaagt attttctaag tattgtaaaa ctttatttaa aattgtattt tctaaaggtg
    10441 ctatattcag cacctctaga ctatatttaa ataatttatt gtgaactaaa tattattttc
    10501 cgtatacaga ttttaatatt ccatagtaac cttctgttcc tataaataac tgttcttgct
    10561 ctatatactc atctattgtg tgagcaagat tttccttaga aggaccaaat cctatagttt
    10621 ttattccagc ttcacctgca tagtgacttc cattcgtaca gaatgagtat tgagttattt
    10681 ctgaatctat tccagcttct tttaatccct tatatgctgc ttgaacaaat tcatcttctt
    10741 cgtcatataa ccaacctggg aagaatcttt cactttctat agtatttcct gtatagcaat
    10801 cagctttttc aac
//
LOCUS       DBGJFF010000129        10763 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.131, whole genome shotgun sequence.
ACCESSION   DBGJFF010000129 DBGJFF010000000
VERSION     DBGJFF010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10763
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.131"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            322..780
                     /locus_tag="ACOIAF_003146"
     CDS             322..780
                     /locus_tag="ACOIAF_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGZ2670144.1"
                     /translation="MEFINFVSDKVIDLKMDCKTKEDFFKEIHNKVFDLGFVKEEFGE
                     KILSRENVFPTGLNLGDYGVAIPHTDAEYIKEQFISVCTFNEPVVFSSMEDQDEKVPV
                     NLAFVLGLNQPHSQLSVLTELMGIMQNKELVDKLMSSTDKEEVLKTLKSL"
     gene            841..1116
                     /locus_tag="ACOIAF_003147"
     CDS             841..1116
                     /locus_tag="ACOIAF_003147"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGZ2670145.1"
                     /translation="MKKILVACGAGIATSTVVCDKVERLVKENNIQAEVIQCKIAECA
                     TKQEGADLIVSTTILPTTYNIPAIKATAYITGVNTAALDKKILDALK"
     gene            1175..2449
                     /locus_tag="ACOIAF_003148"
     CDS             1175..2449
                     /locus_tag="ACOIAF_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HGZ2670146.1"
                     /translation="MDKLLAVVQYILNMGPTVILPITIVVLGMIFRIKFTKALKSGLT
                     IGIGFVGINLVVNLLTSTLGPAAQGMVERFGLNLTVIDVGWPTTASATWASPVAPILI
                     PVCLLVNLLLLYFNKTKILNVDIWNYWHFIVAGAVGYIVTGSYVWAIFCAILMELLVL
                     FIAEKTAKNVQEFYDLEGIALPTGSTASFAPLGYLVGKFVEKIPGINKIQADPDSIQK
                     RFGIFGEPMMMGIILGIILGALAGYDVAGIFNVGISMGAVMLLMPRMVRILMEGLIPI
                     SESIRDFLQAKYGDRELYIGLDAAVATGHPAVISTALILVPITLFLAVILPGNKVLPF
                     GDLATIPFYMAFIVCFRKGNIVQSVITGTIVIAISLYMATNFADVHTQLLQQANMMPK
                     GTTMVTSIDTGGNLLKWGILKLSQLVSSFGIF"
     gene            2579..3631
                     /locus_tag="ACOIAF_003149"
     CDS             2579..3631
                     /locus_tag="ACOIAF_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HGZ2670147.1"
                     /translation="MKAAVLHGTNDMRFEDIEIKPCESDEVKIKVMAAGICGSDPPRV
                     LKHWKYPVPAIPGHEFSGVIAEVGKDVKNVKVGDRVVAIPFIPCNECEYCKRGLFSLC
                     DDHGMLGAKSFGAFAEYVNIKATNVLPIGDMDFEDAAMIEPLAVAMHGVLNIGVQVGD
                     TVAVMGSGTMGQLVIQGLKIAGAGTIIAVDISDNKLRESKELGADIIINAKDINPVEK
                     IKELTGGKGVDIALECAGSKITQEQCLLITKKKSKIGFLGIAYSDITLSEEAFENIFR
                     KELELKGFWNSYSAPFPGQEWTKGINLVNEGKIKLKEMVSHRFSLEDTYKAFEMIRDR
                     KEEFNKILILPQGVEK"
     gene            3631..4662
                     /locus_tag="ACOIAF_003150"
     CDS             3631..4662
                     /locus_tag="ACOIAF_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HGZ2670148.1"
                     /translation="MKAVVKTKPGYDNVEVLEVEEPKATGDKVKIKVEYSGICGSDIH
                     SFKGEYANIKAPVTLGHEFSGIVVEVGEDVKNIKIGDRVTSETTFATCEKCIYCETKD
                     YNLCPTRKGIGTQVNGSFAEYVLSREESIHVLPENVSLLSAALTEPLACCVHAALEKT
                     TIESSDTVLIIGPGPIGLLLAQVVKSQGATVIMSGVTKDKERLEIAKNLGVDRTVNVM
                     EESLADVVNEMTDGNGVNKGFDCSGFMPAVNEALRLLRKKGTFVQVGIFAKKLNEMDQ
                     EAIIQRELQYIGSRSQKPSSWITALDLMASGKVNTEALVTRTVGLDEFKEGITALMNG
                     EEIKVAVKS"
     gene            4754..5566
                     /locus_tag="ACOIAF_003151"
     CDS             4754..5566
                     /locus_tag="ACOIAF_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HGZ2670149.1"
                     /translation="MSTLRDHSNNIRKNIIKMVAEAKSGHPGGSLSIADMLTVLYFDK
                     MNVCAENPKMADRDRFVLSKGHAAPALYATLAEKGFFPEEELITLRKFGSKLQGHPDM
                     KKVAGIDMSTGSLGQGLSAANGMALAGKLDNKDYTVYTILGDGEIQEGQIWEAAMTAS
                     HYKLDNLIAFVDLNGLQIDGSNEEVMNVNPVDDKFKSFGWNVIVINGHSFEEIGNAVD
                     EAKKVTGKPTVIIAKTVKGKGVSFMENNAAWHGTAPNAEQAEQALKDLEGGL"
     gene            5569..6489
                     /locus_tag="ACOIAF_003152"
     CDS             5569..6489
                     /locus_tag="ACOIAF_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HGZ2670150.1"
                     /translation="MGIATREAYGQVLKELAENKDIVVLDADLGKATKSISFKEVAPD
                     RFFDMGIAEGDMIGTAAGLATCGKIPFASTFAIFAAGRGYEQIRNSVAYPNLNVKIAA
                     THAGVTVGEDGGSHQAIEDISLMRGIPNMVVLNPADALEARQAILASIDYNGPVYIRL
                     GRAATPDVNSENYKFEIGKGTVLREGSDITVIATGIMVAKALEAAEELAKEGINVEVV
                     NISTIKPLDEALIKETAKKTGKVVTAEEHSIIGGLGSAVCEALAETKDVVVRRIGVKD
                     VFGQSGTPADLLKHYGLTTEDIVKNIKELL"
     gene            6664..7110
                     /gene="rpiB"
                     /locus_tag="ACOIAF_003153"
     CDS             6664..7110
                     /gene="rpiB"
                     /locus_tag="ACOIAF_003153"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HGZ2670151.1"
                     /translation="MKISIGCDHGGYELKEYIKENLINKGIEVEDVGTNSKESVDFPI
                     YAKKVSQNVQSKKSDLGILCCGTGIGMSIAANKFKGIRAAVVSDCFSAKATREHNNTN
                     VLCLGERVIGKGLAMRIVEEWLNSSYNGERHARRLQMIDDIEKENL"
     gene            7140..7805
                     /locus_tag="ACOIAF_003154"
     CDS             7140..7805
                     /locus_tag="ACOIAF_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HGZ2670152.1"
                     /translation="MSIICASIMCADQMKLKEELEALEEAGVKLLHCDVMDGIFVKNL
                     AMGPELLKSISENTTIPLDIHLATETPDKYIDMMSYIKPKYISFHVESSTNVKADIQK
                     LRNYGIGPVLAISPQTSVDKIEEYISLVEGILVMTVNPGFAGQKFNLSVLDKLDKLTE
                     ILKDYDNPPFIEVDGNINKDTIKLMNGKKVDIYVVGTSALFNDKPPISYKDKIEELKE
                     SIK"
     gene            7915..9417
                     /locus_tag="ACOIAF_003155"
     CDS             7915..9417
                     /locus_tag="ACOIAF_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HGZ2670153.1"
                     /translation="MGDLYKLQNGTDIRGIAYENDSKEVNLTVEEVKKIAKAFHIWLK
                     EKTKKDKVTVAIGTDSRITGSQFRSTVIETLTNDDCNVIDCEIATTPAMFMTTIMDGY
                     NSDGAIMITASHLPYYYNGLKFFTESGGLEKTDIKEMLDIAVKNDSVDYEEVNKKGEV
                     IVKNLIEDYSNLLIDKIRKGVNSSKNYEKPFSGLKILVDAGNGAGGFFAEKVLHILGA
                     DTTGSQFLNPDGMFPNHIPNPENKEAMESICKAVLDNKSDLGIIFDTDVDRAAIVGKN
                     GKPINKNALIAVISSIVLEEHPKTAIVTDSITSEGLAKFINELQGRHHRFKRGYKNVI
                     NEAIRLNSEGEECHLAIETSGHAALKENYFLDDGAYLIAKILIKVAKLSLEGKTIEEL
                     IENLEEAQEEKEVRIGINKEDFRPYAEDILKDLDSHVKSIDGWSVAPNNFEGIRVNCD
                     ESNGDGWFLLRISLHEPLLALNIESNKIGGAEQIYSKLKLFLEKYDLKGL"
     gene            complement(9729..>10763)
                     /locus_tag="ACOIAF_003156"
     CDS             complement(9729..>10763)
                     /locus_tag="ACOIAF_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HGZ2670154.1"
                     /translation="YNKKTINKKLAIVFLISIVVQAIIQYLVLIFFMYIYYAEAIAIV
                     ILFTLVNTVFHFYIYNFLSVKFNYINNISKNIKNIENGNLKYKLEVIGDDEISTLAKS
                     INNITGGLETAVDANIKNERMKAELITNVSHDLKTPLTSILSYVDMLKNNDFDRETIN
                     DYINILDKKSQRLKLLIDDIFEASKLSSGDVEFNITKTDIRELLIQSIVEFEDKIQNS
                     SLDFIIETPEYAVFTMIDGKKTWRVFENLICNILKYSMPNTRVYIDMFIKEENIILTF
                     KNISNDKLNLKPEELIERFRRGDISRKTDGSGLGLSIAQNIIELENAHMEIIIDADLF
                     KVMLTFRRVT"
BASE COUNT         4303 a         1218 c         1966 g         3276 t
ORIGIN      
        1 ttcataataa ataattttat aaaatagttg ttaaacataa aaaaccattt gtaaaaaaca
       61 aaaaaatcat ttctctaaca aaaaaaacca caaaaaatat aaaaaagtat ggctagttaa
      121 gtgattaaca agtaaaaatt aaaataaaaa tcaaaaaaaa tttatcaatg aaagtcagct
      181 aaaatcctgg aagagagcat tatttttggg aaaacatttt cttttgtttc acaaaaaaag
      241 ccactttttg gcacacaaca tgctttatat aaagtcaaga aaaactaagg tataagacaa
      301 aggcaattag gaggtgtaaa tatggagttt ataaattttg taagtgataa agttatagac
      361 cttaaaatgg attgtaaaac gaaagaagat ttctttaaag aaatacataa taaagttttt
      421 gatttaggct ttgtaaaaga agaatttgga gaaaaaatat tatcaagaga aaatgtattt
      481 ccaacaggtt taaatttagg agattatgga gtagcaatac cacatacaga tgcagaatat
      541 attaaagagc aatttatatc agtctgtact tttaatgaac cagtagtgtt tagttctatg
      601 gaagaccaag atgaaaaagt acctgtaaat ttggctttcg tattaggact taatcaacca
      661 cacagtcaat tatctgtatt aacagaatta atgggaatta tgcaaaataa agagttagtt
      721 gataagttga tgagttctac tgataaagaa gaagttttaa agacactaaa gtctttataa
      781 aaataaaaaa gtactaaaaa taaaaaatat aaaaataaaa attattagga ggaaataaaa
      841 atgaaaaaaa tattagtagc atgtggagca ggaatagcaa catcaacagt agtttgtgac
      901 aaggtagaga gattagttaa ggaaaataat atacaagcag aagttataca atgcaaaata
      961 gctgaatgtg caacaaagca agaaggagca gatttaatag tatcaacaac tatactacca
     1021 acaacttata atataccagc aataaaagca acagcatata taactggagt aaatacagca
     1081 gcattagata aaaaaatatt agatgcttta aaataatcct atcatagata cattgaaata
     1141 atattttata aaacagaaaa aggaggattt ataaatggat aaattattag cagtagtaca
     1201 atacatttta aatatgggac cgacagtaat actaccaata acaattgtag tactaggaat
     1261 gatatttaga attaagttta caaaagcttt aaagtcaggt cttacaattg gtataggatt
     1321 tgtgggtata aacttagttg taaacctatt aacatcaaca ctagggccag cggctcaagg
     1381 aatggttgaa agattcggac ttaatttaac agtaatagat gttggttggc caacaacagc
     1441 atcagcaaca tgggcttcac cagttgcacc aatattaata ccagtttgtt tattagtaaa
     1501 cttactatta ttatacttca ataaaacaaa gatattaaac gttgatatat ggaattattg
     1561 gcactttata gtagcaggtg cagttggata tatagtaaca ggaagttatg tttgggctat
     1621 attctgtgca atactaatgg agttattagt actgtttatt gcagaaaaga cagcaaaaaa
     1681 tgttcaggag ttttatgatt tagaaggtat agcattacca actggttcaa cagcttcatt
     1741 tgctccactt ggatatttag taggtaaatt tgttgaaaaa ataccaggaa taaataaaat
     1801 acaagcagac ccagactcta tacaaaaaag atttggtata tttggagaac caatgatgat
     1861 ggggattata ctaggtataa tacttggagc acttgcggga tatgatgtag caggaatatt
     1921 caatgtaggt atatcaatgg gtgcagtaat gttacttatg cctagaatgg ttagaatatt
     1981 aatggaaggt ttaattccaa tttcagaatc aataagagat ttcttacaag caaaatatgg
     2041 agacagagaa ctttatatag gtctggatgc agcagtagca actggacatc cagcagttat
     2101 atcaacagct cttatacttg ttcctataac tttgttctta gcagttatac taccaggaaa
     2161 caaagtttta ccatttggag acttagcgac aataccattt tatatggcat ttatagtgtg
     2221 ctttagaaaa ggtaatatag ttcaatcagt tataacagga acaatagtaa tagctatatc
     2281 actttatatg gctacaaact ttgcagatgt tcatacacaa ttattacaac aagcaaatat
     2341 gatgccaaaa ggaacaacaa tggttacaag tatagataca ggtggaaact tgctaaaatg
     2401 gggaatattg aaattatctc aattagtttc ttcatttgga atattctaat atgtagttaa
     2461 tagagattac tcttttttag taaagtatgc gaaaatttaa aatttagcag ctactaaaaa
     2521 agagtaatat tttttataaa tttttaaaaa tacaaaatac aagaggagga gatttatcgt
     2581 gaaagcagca gtattacatg gcacaaatga tatgagattt gaagatatag aaataaaacc
     2641 atgtgaaagt gatgaagtaa aaataaaagt tatggcagca ggaatatgtg ggtcagatcc
     2701 tccaagagta ttaaaacatt ggaaatatcc agttccagct ataccaggac atgagttttc
     2761 tggagtaatt gctgaagttg gtaaggatgt aaaaaatgta aaagttggag atagagtagt
     2821 tgcaattcct tttatacctt gtaatgaatg tgaatattgt aaaagaggat tattttcatt
     2881 gtgtgatgac catggaatgt taggagctaa aagttttgga gcttttgcag agtatgtaaa
     2941 tataaaagct acaaatgtac ttccaatagg agatatggac tttgaagatg ctgcaatgat
     3001 agagccacta gcagttgcta tgcatggagt acttaatata ggtgttcaag ttggagacac
     3061 tgtagccgta atgggtagtg gaacaatggg gcagttggta atacaaggac ttaaaatagc
     3121 aggggcagga acaattatag cagttgatat atctgataat aaattaagag aatcaaagga
     3181 attaggggct gatataataa ttaatgctaa agatattaac ccagtagaaa aaataaaaga
     3241 acttactggt ggaaaaggtg ttgacatagc tcttgaatgt gcaggttcaa aaataactca
     3301 agaacaatgt ttacttataa ctaaaaagaa aagtaagata ggatttttag ggatagcata
     3361 ttcagatata actttatcag aagaagcatt tgaaaatata tttagaaaag aactagaatt
     3421 aaaaggtttt tggaactctt attctgcacc atttccagga caagaatgga ctaagggaat
     3481 caatctagtt aatgaaggaa agataaaatt aaaagaaatg gtatctcata gattctcact
     3541 tgaagatact tacaaagcat ttgaaatgat aagagataga aaagaagaat ttaacaaaat
     3601 attaatttta ccacaagggg ttgaaaaata atgaaagcag tagttaaaac taaaccaggc
     3661 tatgataatg tagaagtttt agaagtagaa gaaccaaaag ctacaggaga caaggttaaa
     3721 ataaaagtag aatatagtgg tatatgtggt tcagatatac attcatttaa aggtgaatat
     3781 gcaaatataa aagccccagt aacattaggt catgaatttt ctggaattgt tgtagaagta
     3841 ggagaagatg ttaaaaatat aaaaatagga gatagagtta catcagagac tacttttgca
     3901 acttgtgaga aatgtatata ttgtgaaact aaagattata acttatgccc aactagaaaa
     3961 ggtattggta ctcaagtaaa tggaagtttt gctgagtatg tattatcaag agaagaaagc
     4021 atacatgtac ttcctgaaaa tgtatcttta ttatcagcag cccttacaga gccactagct
     4081 tgttgtgttc atgcagctct tgaaaagact actatagaaa gttctgatac agttttaata
     4141 ataggaccag gaccaattgg tttattatta gctcaagttg ttaaatctca aggtgctaca
     4201 gttataatgt ctggtgttac aaaagataag gaaagattag aaatagctaa aaatcttgga
     4261 gtagatagaa ctgtaaatgt aatggaagaa agtttggcag atgtagtgaa tgaaatgact
     4321 gatggaaatg gagtaaacaa aggatttgat tgttctgggt ttatgccagc agttaacgaa
     4381 gccttaagac tgcttagaaa aaaaggtact ttcgtacaag ttggaatctt tgctaaaaaa
     4441 ttaaatgaaa tggaccaaga agcaataatc caaagagagt tacaatatat aggaagtaga
     4501 tctcaaaagc caagttcttg gattacagca ttagatttaa tggcaagtgg gaaagtaaat
     4561 acagaagctt tagttactag aactgttggt ttagacgaat ttaaagaagg tattacagct
     4621 ttaatgaacg gggaagaaat aaaagtagca gtaaaatctt aaaaaaacat ttgtattatt
     4681 ttaaaaaaaa ttgtaaaatc tactgtagga taaaaaaatg taaggtctta aaaaataaca
     4741 ggaggaagaa agtatgagca ctcttagaga tcattctaat aatattagaa aaaacataat
     4801 aaaaatggtt gcagaagcta aaagcggaca tccaggtggt tcattatcaa tagctgatat
     4861 gttgacagta ttatattttg ataagatgaa cgtatgtgca gaaaatccaa aaatggctga
     4921 tagagataga tttgttctat caaaaggtca tgcagcacca gctttatatg ctacattagc
     4981 agaaaaagga tttttcccag aagaagaatt aataacacta agaaaatttg gttctaagtt
     5041 acaaggtcac ccagacatga aaaaagtagc aggaatcgat atgtctactg gttctcttgg
     5101 tcaagggcta tctgcagcaa atggtatggc acttgcagga aaattagata acaaagacta
     5161 tacagtatat actatattag gtgatggaga aatccaagaa ggtcaaatat gggaagctgc
     5221 aatgacagca tcacattaca aattagataa tttaattgca tttgttgact taaatggatt
     5281 acaaatagat ggttctaatg aagaagtaat gaatgtaaat ccagtagatg acaaattcaa
     5341 atcttttgga tggaatgtaa tagtaataaa tggacattct tttgaagaaa taggaaatgc
     5401 agttgatgaa gctaaaaaag ttacaggaaa accaactgta ataatagcta aaacagtaaa
     5461 aggtaaaggt gtttcattta tggaaaataa tgctgcatgg catggaacag ctccaaatgc
     5521 agaacaagca gagcaagcat tgaaagattt agaaggaggt ctataggaat gggaatagca
     5581 actagagaag cttacggaca agtgttaaaa gaacttgcag aaaataaaga tatagtagta
     5641 ttagatgcag atttaggaaa agctacaaaa agtatatcat ttaaagaagt tgccccagat
     5701 agattttttg acatggggat agcagaaggt gacatgattg gtacagcagc aggtcttgct
     5761 acttgtggaa aaataccttt tgcgagtact tttgcaatat ttgcagcagg aagaggctat
     5821 gagcaaataa gaaactctgt agcatatcca aatttaaatg taaaaatagc agctactcat
     5881 gcaggtgtaa cagtaggtga agatggtggt agccatcaag ctatagagga tatatctcta
     5941 atgagaggta taccaaatat ggttgtatta aatccagcag atgctttaga agcaagacaa
     6001 gctattcttg catctattga ttataatgga cctgtttata taagattagg tagagctgca
     6061 actcctgatg taaacagtga aaattataaa tttgaaatag gaaaaggaac tgttttaaga
     6121 gaaggttctg acataacagt aatagctaca ggaataatgg tagcaaaagc cttagaagca
     6181 gcagaagaac ttgctaaaga aggtataaat gtagaagttg ttaatatatc tacaataaaa
     6241 ccattagatg aagcattaat aaaagaaact gctaagaaaa ctggaaaagt agttacagca
     6301 gaggagcaca gtataatagg tggtttaggt tcagctgtat gtgaagcttt agcagaaact
     6361 aaagatgtag tagttagaag aattggtgtt aaagatgtat ttggtcaatc aggtacacca
     6421 gctgatttat taaaacacta tggtttaact acagaagata tagtaaaaaa tataaaagag
     6481 ttgttataga tataaatata tctaaataac aaattgattt gacataagtc aattaaagct
     6541 gtcttaaaaa tagaaagaaa tttctacaat gggacagctt tatttttaca atactttatt
     6601 tttatgataa ttataaaata gtctgaaagt aatcaaaaaa attataaaaa gaggtgctat
     6661 ttaatgaaaa tatctattgg atgtgaccat ggtggttatg aattaaagga atatataaag
     6721 gaaaatttaa taaataaagg tatagaagtt gaagatgtgg gtacaaattc aaaagaaagt
     6781 gtagattttc caatatatgc aaaaaaggta agtcaaaatg ttcaaagtaa aaaatctgat
     6841 ttaggcatac tttgttgtgg aacaggaata ggtatgtcta ttgcagcaaa taagtttaaa
     6901 ggaatcagag cagctgttgt ttctgactgt ttttcagcta aggcaactag agaacacaat
     6961 aatacaaatg tactttgttt aggagaaaga gttattggga aaggattagc aatgagaatt
     7021 gttgaagaat ggttaaattc aagttataat ggagaaagac atgcaagaag attacaaatg
     7081 attgatgata tagaaaaaga aaatttataa atatataatt tataacaagg gggagcgtta
     7141 tgtctattat ttgtgcttca ataatgtgtg cagaccaaat gaagttaaaa gaggaattag
     7201 aagctcttga agaagcaggt gtaaaattac ttcattgtga tgttatggat ggaatatttg
     7261 ttaaaaactt ggctatggga ccagagttat taaaatcaat aagtgaaaat acaacaatac
     7321 ctttagacat acacttagcc acagaaactc cagataaata tattgatatg atgtcttata
     7381 taaagccaaa atatatttct tttcatgtag aatcaagtac aaatgtaaaa gctgatatac
     7441 aaaagttaag aaattatggt atagggccag tgttagcaat aagccctcaa acaagtgtgg
     7501 ataaaataga agaatatata agtttagttg agggtatctt agtaatgaca gtaaatccag
     7561 ggtttgcagg tcaaaagttt aatctttctg tattggataa gttggataaa cttacagaga
     7621 tactaaaaga ttatgataat cctccattta tagaggttga tggaaatata aataaggata
     7681 ctattaaact tatgaatggt aaaaaagtag atatatatgt tgttggtact tcagctttat
     7741 ttaatgataa gcctccaatt tcatataaag ataaaataga agaactaaaa gaaagtataa
     7801 agtaaccatg taataaaatt ataatatgtt tctgtttaat agtaaaaatt taggtggaaa
     7861 caagaaattt tataatattt aataaaaatt ttggttgagt aaggagaatt ttttatggga
     7921 gacctatata aactacaaaa tggtactgat attagaggaa tagcgtatga aaatgattct
     7981 aaagaagtta acctaactgt agaagaagta aaaaaaatag ctaaagcgtt tcatatttgg
     8041 ttaaaagaaa agactaagaa agataaagta actgttgcca taggaactga ttctaggata
     8101 acaggaagcc aatttagaag tactgttatt gaaactttaa ctaatgatga ttgtaatgta
     8161 attgattgtg agattgccac aactccagct atgtttatga caactattat ggatggatac
     8221 aattctgatg gggcgattat gattacagca agtcacttac cttattacta taatggttta
     8281 aagtttttca cagaaagtgg aggtttagaa aaaactgata taaaagagat gttggatata
     8341 gctgttaaaa atgatagtgt agactatgag gaagtaaata aaaaaggtga agttatagta
     8401 aaaaatttaa tagaagatta ttctaattta ttaatagata aaattagaaa aggtgtaaat
     8461 tcaagtaaaa attatgaaaa gcctttttct gggcttaaga tattggttga tgcaggaaat
     8521 ggggctggtg gattctttgc tgaaaaagtt ttacacatac tgggagcaga tacgacaggt
     8581 agtcagtttt tgaacccaga tggtatgttc ccaaatcata tacctaatcc cgaaaacaaa
     8641 gaagcaatgg aaagcatatg taaagctgtg ttagataata agagcgacct aggaataatc
     8701 tttgatacag atgtagacag agctgctatt gtaggtaaaa atggcaaacc aataaataaa
     8761 aatgctctta tagctgttat atcatcaata gtattagaag aacatccaaa aacagctata
     8821 gtaacagatt cgattacttc tgaaggactt gctaaattta taaatgaatt acaaggaaga
     8881 catcatagat ttaaaagagg atataaaaat gtaataaatg aagctataag attaaatagt
     8941 gaaggtgaag agtgccattt agctatagaa acgtctggac atgctgcttt aaaagaaaat
     9001 tatttcttag atgatggggc atatttaata gctaaaattt taataaaagt agcaaagcta
     9061 tccttagaag gaaagactat agaagaattg atagaaaacc ttgaagaagc tcaagaagag
     9121 aaagaagtta gaataggcat taataaagaa gattttagac catatgcaga ggatatatta
     9181 aaagatttag attctcatgt caagagtatt gatggatggt ctgtagcacc aaataatttt
     9241 gaaggcataa gagttaattg tgatgaatca aatggagatg gatggttttt acttagaata
     9301 tcactacatg aaccattatt agctttaaat attgaatcaa ataaaattgg tggagcagaa
     9361 caaatttatt ctaagttaaa acttttctta gaaaaatatg atttaaaagg tctttagggt
     9421 aaatagaaag aatatattta tattgaagaa caaatgtaaa tttgtaccta agattacaaa
     9481 tattacaaaa ttgtaatctt aggtaacaga gatgtaactg attatggaaa cactatgtga
     9541 tattatagat acagagagat gaaatatatg atgaattata gagaaatata atttaaaatg
     9601 tattaatatc acaccaaaaa ggtataatca aaaaatatgt aaatataaac cccaacattt
     9661 agaaaaagac ctctaaggag gtttttttct tgtttaaata acttaaaata tatttttata
     9721 ttaatatatc aagtaactct tctaaaagta agcattacct taaataaatc agcatctata
     9781 attatttcca tgtgtgcatt ttctaattca attatatttt gagcaattga taaaccaaga
     9841 ccagagccat ctgtttttct agatatatca ccccttctaa atctttctat taactcttct
     9901 ggctttagat ttagtttatc atttgaaata tttttaaacg ttaatattat attttcttcc
     9961 tttataaaca tatctatgta gaccctagtg ttaggcatag agtattttag tatattgcaa
    10021 attagattct caaaaactct ccaagttttt ttaccatcta tcatggtaaa aacagcatat
    10081 tcaggtgttt ctattataaa atctaaactg ctattttgaa ttttgtcttc aaactctaca
    10141 atactttgaa ttaaaagttc tcttatatcg gtttttgtta tattaaattc tacatctcca
    10201 ctacttagct tagatgcttc aaatatgtca tctattaata attttaatct ttgtgatttt
    10261 ttatctaaaa tatttatata gtcatttata gtctctctgt caaagtcatt attttttaac
    10321 atatcaacat agcttaatat agatgtaagt ggagttttta agtcatgaga tacgtttgtg
    10381 ataagctctg ccttcatcct ttcatttttt atattagcat ctacagcagt ttcaagaccg
    10441 cctgttatat tatttatact tttggctaaa gtagatattt catcatctcc tataacttct
    10501 aatttatact ttaaattgcc attttctata ttttttatat tttttgatat gttatttatg
    10561 taattaaact taacagataa aaagttgtat atataaaaat gaaaaactgt gtttacaagt
    10621 gtaaataaaa ttactatagc tattgcctca gcataatata tatacataaa gaatatcaaa
    10681 acaagatatt ggataatagc ttgtacaact atagatatta aaaatactat agctaatttt
    10741 ttatttatag tttttttatt ata
//
LOCUS       DBGJFF010000130        10724 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.132, whole genome shotgun sequence.
ACCESSION   DBGJFF010000130 DBGJFF010000000
VERSION     DBGJFF010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10724
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.132"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            103..837
                     /locus_tag="ACOIAF_003157"
     CDS             103..837
                     /locus_tag="ACOIAF_003157"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGZ2670155.1"
                     /translation="MKQNKYDDDRFFNKYSKMERSTNGLAGAGEWHVLKKMLPDFKGK
                     RVLDLGCGFGWHCQYAVENGAISAVGVDISEKMLKEARNKTQLDNINYICMPIEDINF
                     PKDSFDVVISSLAFHYIQSFEDICKNISNCLSNKGNFVFSVEHPIFTAYGSQEWYCDK
                     DGNNLHWPVDRYFEEGVRRSNFLGEEVIKYHKTLTTYLNALMKTGFEILEIIEPQPEE
                     KLLDTIPDMKDELRRPMMLLVSAKKR"
     gene            complement(980..1471)
                     /locus_tag="ACOIAF_003158"
     CDS             complement(980..1471)
                     /locus_tag="ACOIAF_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HGZ2670156.1"
                     /translation="MNNQQQEVYKKLDELSIPYEAVNHPPVYTIEEMEELKTLHMEFV
                     VKNLFLRDAKGKEHFLVVLGKDKKADLKDIRQQIDSKPLSFASEERLQKYLKLTKGAV
                     TPFGILNDKEAVVKVVFDKDLLKMDKIGIHPNDNTATVFLKFEDMKKLIEENGNEIYY
                     VEV"
     gene            1820..2203
                     /locus_tag="ACOIAF_003159"
     CDS             1820..2203
                     /locus_tag="ACOIAF_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBECR2 nuclease fold domain-containing protein"
                     /protein_id="HGZ2670157.1"
                     /translation="MSTDKVVGIIDEETAELAGIEYTGKIYASSGVIKHIKKKHRCQL
                     SKDIFNDIIDTIKMVLKSPEYIGSHPKKTGKSVEFIKKIDDYILVATELDTKKGYLYV
                     SSMYAIKEAKLESRVNSGRVKQYDG"
     gene            2711..3094
                     /locus_tag="ACOIAF_003160"
     CDS             2711..3094
                     /locus_tag="ACOIAF_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HGZ2670158.1"
                     /translation="MNLSKKIEDKRKAKQRAEKVKKAKIATAGVVLGAVTGAVSGVLL
                     APKSGKETREDIKDASQQIAEKINMKTVDVKGKVSEKLEDKKGNFIESKKKIKKYIDN
                     KKSVENSSEEDKIIDIVEPVSVTEE"
     gene            3096..3446
                     /locus_tag="ACOIAF_003161"
     CDS             3096..3446
                     /locus_tag="ACOIAF_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670159.1"
                     /translation="MDNTITINISIIYVLLAILACIALVYLIIVLVKLHKILNNVGTM
                     LNENTQNINTTLNNLPNVVETFGEVGENMKDVTEVVTEVTADFLVTKDSVKSNMDVIV
                     DILTIIKNIFVDKK"
     gene            complement(3547..4005)
                     /locus_tag="ACOIAF_003162"
     CDS             complement(3547..4005)
                     /locus_tag="ACOIAF_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670160.1"
                     /translation="MIRTKVVELIATVCRENKPHKWVDENYTPYDKSGKVELMSIEDL
                     NELISSSGRADFLYSSRLQKLLNEVYINQSRASYISGCGLFWSSYWDILEEKFEEWLY
                     NSYIFFDEDDEYLEGMEDFELECKDVLMDVIETTSIDIYVQMIKRNITNY"
     gene            complement(4050..4838)
                     /locus_tag="ACOIAF_003163"
     CDS             complement(4050..4838)
                     /locus_tag="ACOIAF_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HGZ2670161.1"
                     /translation="MKVVIIPGHTLIGKGTGAVGYINESKETRILNDLIVKWLKIGGA
                     TVYTGRVDESSNHLADQCAIANKQETDLAVQIHFNSNATTSTPVGTETIYKTNNGKTY
                     AERVNTRLATVFKDRGAKSDVRGLYWLNHTIAPAILIEVCFVDSKADTDYYVNNKDKV
                     AKLIAEGILNKSISNSQGGGENKVYENVIVYTGDADKVAAQILHWQLKDSLIIEASSY
                     KQGLGKKVYVVGGEANKLVKGDVVINGADRYETVKLALQEIDKL"
     gene            complement(4977..5414)
                     /locus_tag="ACOIAF_003164"
     CDS             complement(4977..5414)
                     /locus_tag="ACOIAF_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HGZ2670162.1"
                     /translation="MNKKLENRCSWQEYFMRLCETVAERGTCDRAYVGAIIVNSENRI
                     VSTGYNGSISGDKHCSEVGHEMRDGHCIRTIHAEQNALYYCAKEGISVKNCSIYVTHF
                     PCLNCTKAIIQAGIKHIYYRTGYRIDEYAIKLLQSSNVLYTKL"
     gene            complement(5648..5782)
                     /locus_tag="ACOIAF_003165"
     CDS             complement(5648..5782)
                     /locus_tag="ACOIAF_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670163.1"
                     /translation="MGILEGAAMIREIAIKIAKEKGITEQKAWPEAVKEFKEKYELVL
                     "
     gene            6236..6745
                     /locus_tag="ACOIAF_003166"
     CDS             6236..6745
                     /locus_tag="ACOIAF_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGZ2670164.1"
                     /translation="MLSENIKTIRKSKGLSQQELAIKLNVVRQTISKWEQGLSVPDSD
                     MLISISEVLETSVSTLLGETIVESEVDNLKVISEKLEIINLQLAQRKNMRRKMFHWLF
                     ILLCAVIVIVFVVLGGLNSPYLDWNYTDPETAVFGVAFHAFEWLFVRLVPIVLIGAII
                     GIFLTRKKI"
     gene            6772..6918
                     /locus_tag="ACOIAF_003167"
                     /pseudo
     CDS             6772..6918
                     /locus_tag="ACOIAF_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            7150..7686
                     /locus_tag="ACOIAF_003168"
     CDS             7150..7686
                     /locus_tag="ACOIAF_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HGZ2670165.1"
                     /translation="MQGGVNIRQVKSFVFPVISLIFLDQISKVLIGLFLMDFEIDIIG
                     KFLRFNPVQNTNLSYGGNFIGILSNLWVLVLFNLLVILVIISGYAFYKSKNEQTSYSV
                     KVIMSCGLAGTICSLIDKLFWGGSLDFLQIPSFFIFDLKDCYLTVAEIIFVVIGILHN
                     REISMKEYIYFCYRQFKR"
     gene            complement(8126..8926)
                     /locus_tag="ACOIAF_003169"
     CDS             complement(8126..8926)
                     /locus_tag="ACOIAF_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2670166.1"
                     /translation="MRLNKRSEGYINYVKGVKREKRNVLFYQLLILIGFIVIWELLAD
                     LNVINTFLFSKPSDIYNLFIQYAFSGQLFKHIGISVYETVLGLVIGTVLGILVAIALW
                     WSEKLSKILDPFLVVLNALPKTALAPIIIVWVGAGIEGIVVTAVTISVVVTILSAYNY
                     FMNIDEEKIKMLKSFGATKSQILFKLILPANTGNLINLTKINIGMAWVGVIVGEFLVS
                     RYGIGYLIVYGSQVFKLDLVMMGVFVLAICAWAMYAVVNIIEKIYNSR"
     gene            complement(9121..>10724)
                     /locus_tag="ACOIAF_003170"
     CDS             complement(9121..>10724)
                     /locus_tag="ACOIAF_003170"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HGZ2670167.1"
                     /translation="ILKAAIYSRKSRFTGKGDSIENQIQLCIDYAKSIGVKDFLIYED
                     EGFSGSNIDRPQFKKMMQDAKDKKFSYLVCYRLDRISRNVSDFSTLIEKLNELNISFI
                     SIKEQFDTSTPMGRAMMFISSVFAQLERETIAERIKDNMYELARSGRWLGGNTPHGFN
                     SEKITFLDENLKERSMYKLQVNDEQMEIVKLIYNKYLELRSLSKLYKYVYNNGIRGPR
                     GGKFDPSSLSRILRNPAYVKANDEILDYLKNSGMDVVGTPDRKCGILTYAKNTSNSIA
                     AIAKHHGVIEPEIWLEVQNQLDKNRDKTPRISTGKTALLSGLIKCGKCGSNMRITYKG
                     KKTDKDLKYYYICGTKKTLGSDSCNCKNLNGPLVESLVIDKIKNCKEESIINAFNSNN
                     IKTDNVFNIFKSETNLLKNQILDKERLIKNLVIELAKNTGSTASDYIVSQIESLNNEI
                     SILKDKINTLESSNINISSDSINIDIIVSNLKRFNDEVDNASLDDKRLLLSTIINSIV
                     WDSSLGNLNIVYMGVEVDNSVSLSN"
BASE COUNT         3882 a         1524 c         1416 g         3902 t
ORIGIN      
        1 tttatagaaa cttttattat tttatataaa agtgataaaa acttatattt tgtatttaat
       61 agagtgttgc taagtgaata tatttaaaat ggaggaaact ttatgaaaca aaataaatat
      121 gatgatgata gattttttaa taaatacagt aaaatggaac gttctacaaa tggattagca
      181 ggtgctggag agtggcatgt tttaaaaaaa atgttacctg atttcaaagg aaaaagagtt
      241 ttagatttag gatgtggatt tggatggcat tgtcaatatg cagttgagaa tggagcaatt
      301 tctgctgttg gagttgatat ttcagaaaaa atgttaaaag aagctagaaa caaaacgcaa
      361 cttgataata taaattacat atgtatgcct attgaagata ttaattttcc aaaagatagt
      421 tttgatgtag ttattagttc cttggcattt cattatattc aatcatttga agatatatgt
      481 aagaatataa gtaattgtct ttcaaataaa ggtaattttg tattttctgt tgaacatcct
      541 atatttactg catatggaag tcaagagtgg tactgtgata aagatggaaa taatctacac
      601 tggccagtag atagatattt tgaagaagga gtacgtaggt caaattttct aggagaagag
      661 gttataaaat atcataaaac attaacaact tatttaaatg cgttaatgaa aacaggattt
      721 gaaatattag aaattattga acctcaacca gaagaaaaac tgttagatac aattcctgat
      781 atgaaagatg agctacgtag acctatgatg ttacttgtat ctgcaaaaaa aagataagta
      841 aatataaata atcattattg tagaaaaaaa gttgtagaaa ataattacac taatggagat
      901 gattatacca gtggtattaa ttacaccagt agtaaggagg caaaagcatt cgttgctttt
      961 gccatttttt aagttgcaat tatacttcta cgtagtatat ttcgtttcca ttttcttcta
     1021 taagcttttt catatcttca aattttaaaa atacagtagc agtattatca tttggatgta
     1081 tacctatttt atccattttt aataaatcct tatcaaatac aacttttaca acagcctctt
     1141 tatcatttaa tataccaaaa ggagtcacag ctcctttagt aagtttcaag tatttttgaa
     1201 gtctttcttc tgatgcaaaa ctaagaggtt tactatcaat ttgttgacga atatctttta
     1261 aatctgcttt tttgtcttta cctaatacaa ctaaaaaatg ttcttttcct ttagcatcac
     1321 gaagaaatag gttttttaca acaaattcca tatgtaatgt ttttaattct tccatttctt
     1381 caattgtata aactggtgga tgatttacag cctcataagg tatgcttaac tcatctaatt
     1441 ttttatatac ttcctgttgt tgattgttca tgatttcctc ccattagaaa tttattttat
     1501 atataaataa tattatcaca gaaaacttta aaagaaaacg ttttatataa tatagacaat
     1561 agattatatt tttataaaga agcaagaata ctcaataaaa cttaaagaaa ttaaacgatt
     1621 tctaaaaata ccttgaaaat agaaccttat ttgccattta taaattatat aaaaagggat
     1681 tatataaagt catatatgaa aaaactatat tatactatct tttctttttg gaataaacta
     1741 tactttaaat atagatattt aatttaaata aattttacag tattaatagt aaattatctt
     1801 agaaaggaat gtgctttata tgtctacaga caaagttgtt ggaatcatag atgaagaaac
     1861 ggcagaactt gcaggaattg aatatactgg taaaatttat gcttcttctg gtgttataaa
     1921 acatataaag aaaaaacata gatgtcaact gagtaaggat atttttaatg atataataga
     1981 cactataaaa atggtattaa agtcacctga atatatagga agtcatccta aaaaaactgg
     2041 aaaaagtgtg gagtttataa aaaaaattga tgattatatt ttagtagcta cagaattaga
     2101 tacaaaaaaa ggatatttat atgtatcttc aatgtatgct ataaaagaag caaaattaga
     2161 aagtagagta aacagtggaa gagtaaaaca atatgatggt taacataata ttattaattt
     2221 tccctaaaat attgaaaaat attattatat aagttataat attaatatac acgtagatat
     2281 ttattagtta gatttgaggt tggaaaaggc acccaatacg ctactataga gtagttaata
     2341 gagatgcagg atacgccgcc ctgccaaatc taattaaatt aaaaagagag gattaattcc
     2401 tctcttattg tttttttgag atactaattt aatctataat agaaagattg aaggtcgttt
     2461 atacaaaaaa attttcagtt atatacatta aactaattaa ataagatatt tataatctat
     2521 atgtgataca tattatatat taataaaaga aaaatatata gaaaatcctt ataaaaattt
     2581 aaattgtaat gaataggaag taattaaaaa gaatttttat tgatttttaa aaatttagtt
     2641 agaaactata tcattatggt aaaaaatgta ttataatgtt tagaaatatt ataataagga
     2701 gcgattaaac atgaatctat cgaagaaaat agaagataaa agaaaagcta aacagagagc
     2761 tgaaaaagtt aagaaagcta aaattgctac agctggtgtg gtattaggag ctgttactgg
     2821 tgcagtgagt ggagtacttt tggctcctaa atctggaaaa gaaacaagag aggatataaa
     2881 agatgcttca caacaaattg ctgaaaagat aaatatgaaa actgtagatg taaaaggcaa
     2941 agtatctgag aagctagagg ataaaaaagg aaactttata gaatcaaaga aaaaaataaa
     3001 aaaatatata gataacaaaa agagtgttga aaattctagt gaggaagata aaataataga
     3061 tatagttgaa ccagttagtg ttactgagga ataatatgga caatacaata acgattaaca
     3121 taagtataat atatgtatta ttagctatac ttgcttgtat tgctttagtt tatttaatta
     3181 ttgtacttgt taaactacat aagattttaa ataatgttgg aactatgtta aatgaaaata
     3241 ctcaaaatat aaatacaact ttaaataatc ttcctaatgt tgtagaaact tttggagaag
     3301 taggagaaaa tatgaaagat gtaacagaag tagttactga agttacagca gattttcttg
     3361 ttactaaaga cagtgtcaaa tcaaatatgg atgttatagt tgatatattg actataatta
     3421 aaaacatttt tgtagataaa aaataataag ttaatataaa ataagcctat aagatataat
     3481 ttaaacgacc ttaaaatagc atttataagg tcgtttttta ttagcttgat ttgacactat
     3541 gtgtttttaa taattggtaa tattcctctt tatcatttga acatatatat ctatgcttgt
     3601 tgtttcaatt acatccataa gaacatcttt acattcaagc tcaaaatctt ccattccttc
     3661 taaatattca tcatcttcat caaaaaagat ataagaatta tatagccatt cttcaaattt
     3721 ttcttcaagt atatcccaat acgaactcca aaataatcca caacctgata tataagatgc
     3781 tctactttga tttatataaa cttcatttag aagcttttgt aatctactgc tataaagaaa
     3841 atctgctctt ccactgcttg atattaattc atttaaatct tctattgaca ttaattcaac
     3901 tttaccactt ttatcataag gagtataatt ttcatcaacc catttatgtg gtttgttttc
     3961 tctgcatact gttgctatca actctacaac ttttgttctt atcatttttt tatcctaact
     4021 tatttatttt ttataaaata atcatgattt tataatttat ctatttcttg taatgctaat
     4081 ttaacagttt catatctatc agcaccattt attactacat caccttttac aagtttatta
     4141 gcttctccac ctacaacata aaccttttta cctaaacctt gcttataact agacgcttca
     4201 attattaaag aatctttcaa ttgccaatgt aaaatctgag cagccacttt atcagcatca
     4261 cctgtataaa cgattacatt ttcatacact ttattttccc ctcccccttg agaatttgat
     4321 atagatttgt ttagtatacc ttctgctatc aatttagcaa ctttatcttt attattaaca
     4381 taataatctg tatctgcttt actatctaca aaacatactt ctattaatat tgctggtgca
     4441 atagtatgat taagccaata aagacctcta acatcagact ttgcacccct atctttgaat
     4501 actgtagcta acctagtatt cactctttct gcataggttt tgccattatt agtcttatat
     4561 attgtttctg tccctactgg agttgaagtt gtagcatttg aattaaaatg aatctgaaca
     4621 gccaaatcag tttcctgttt atttgctata gcacattggt ctgctaaatg attacttgat
     4681 tcatcaactc ttcctgtata aacagtagct ccacctattt taagccattt aacaattaag
     4741 tcattcagta ttcttgtttc tttgctttcg tttatatacc caacagctcc tgtacctttt
     4801 ccaattaaag tgtgccctgg tattattact actttcaaac taacatctcc tttacaatta
     4861 aatttaactt ctatttaata tatgcataac ctagaatttt gtttcttttt atatcttata
     4921 cttttattat aacccatttt gtaaaaatat agaatattaa gtaaataatg ttaaaattat
     4981 aattttgtat ataagacatt acttgattgc aataatttta tagcatattc atctattcta
     5041 tatccagttc tataatatat atgtttaatt ccagcttgaa ttatagcctt agtacaattt
     5101 aagcatggaa agtgagttac ataaatagag caatttttaa ctgaaatacc ttcttttgca
     5161 caataataaa gggcattttg ttcagcgtga atggttctaa tacaatgtcc atctctcatt
     5221 tcatgaccaa cttcactaca atgcttatct ccagaaatac taccattata tccagtagac
     5281 actattctgt tttctgaatt gacaattata gctcctacat aagctctatc acaagttcct
     5341 ctttcagcta ctgtttcgca cagtctcata aaatattctt gccatgagca tctattttca
     5401 agttttttat tcaaatcata tacctccaaa atttatatct aatatataag atttatacct
     5461 taaaaataat attaaaaacc aatttatatg ataaataaaa tttattaata ctgtttttag
     5521 tctaaattat ttcattttaa attaaagtta caaggaatct aaaattatat atatctttct
     5581 tcgtaatatg aatgttacaa tataaaaaac tgtctcaata aaatagagac agttttacag
     5641 tcaaagttta taatacaagt tcatattttt ctttaaattc ttttacagct tcgggccatg
     5701 ccttttgttc tgtaatacct ttttctttag ctattttaat tgcaatctct cttatcattg
     5761 cagcgccttc taatattccc atttttaatc tcccttcaaa tatctttttg tatagaatat
     5821 aaatagtgct aagatttatt acatgctgca tatacaatat ctatacaaca cataacttgt
     5881 aaattccttt tattttcata cgtctttaac cgacatataa ttatattcaa taagttttaa
     5941 aattatgaac tcaattataa ttatttttat ttttttagaa tctaaacact aaaaattgta
     6001 ggtaaatttg tcttatgcct ttataaatat ttctacagtt ttaattactg gacatatcta
     6061 tttttattat aaaaagcgct taaaaatcga gaatatccgc ataaaaactg tatataagca
     6121 aaacgattaa agtaaaaaga agaaatttac ttgaatgtta aaatgcgttg cttgaagcaa
     6181 tgggcagttt tgcttataat atcggtaaca actgaaagaa aggtggttag ccctaatgtt
     6241 aagcgaaaac attaaaacaa tcaggaagtc aaaaggactt tcacaacaag aacttgctat
     6301 caaactgaat gtagttcgac aaacaatatc taaatgggaa caaggactgt cggttcccga
     6361 ttctgatatg ttgatttcca tatcagaggt gcttgaaacg tccgtaagca ctttacttgg
     6421 agaaactatt gttgagtcgg aagttgataa cttaaaagtg atttctgaaa aattagagat
     6481 tataaacttg caacttgcac aaagaaaaaa catgagaaga aaaatgtttc attggttatt
     6541 tattttatta tgtgcagtca tagtaatagt ttttgtggtc ttagggggac taaatagtcc
     6601 ttacttggat tggaattata ccgaccctga aaccgctgtt tttggagtag cttttcatgc
     6661 ttttgagtgg ctatttgtca gattagtacc gattgtcctt ataggagcaa ttattggaat
     6721 ctttttgaca cgtaagaaaa tatgaagtaa ctctgctttt tataatacag caagcgttcc
     6781 ttaaaagtgt cattctacat ggcaatcgag gacaactatg taaggaagaa cctttttgat
     6841 ttccaactaa gcgaagttat tgaggacgac agtaggctca aaaccgcatt aagtaaagta
     6901 caggaagcaa gactgctgga cttatactct catgcgtcag atgcaggaaa tgcgaggtct
     6961 ggtagcatga tttacaacga tttgtacaac gactacgagt agaaatatat ggagataagt
     7021 attatctacg aaatcagaaa agaaatgact tgataattta ctcatgttat aattattgat
     7081 gtgaaattaa taaatcgaaa ttcctttttt ggatttaaca atactgagta aggaaaaagt
     7141 gaatgacttt tgcaaggagg tgttaatatc agacaagtaa agtcttttgt ttttccagtt
     7201 atatccttaa tattcttaga ccaaattagc aaagttctta taggattatt cttaatggac
     7261 tttgaaattg atataattgg gaaattttta agattcaatc ctgttcaaaa tacaaatctc
     7321 tcttatgggg gaaactttat tggtattcta tctaatttat gggtattggt attgtttaac
     7381 cttttagtta tattagttat tatatctgga tatgcttttt ataaatcaaa aaatgaacaa
     7441 acaagctatt cagtaaaagt aattatgtct tgtggacttg ctggtacaat atgtagcttg
     7501 atagataaat tattttgggg aggaagttta gattttttgc agataccaag cttttttatt
     7561 ttcgacttaa aagactgcta ccttactgtt gcagaaataa tatttgttgt cataggaatt
     7621 ttgcataata gagaaatatc aatgaaagaa tacatatatt tttgctatcg tcagtttaaa
     7681 agataaattt agaagataaa tttaagtttc aaggtaaact tatttaatca cagcaaatag
     7741 ttggcttact taagcttttg ggctaaactg tgagcactaa ttaagtctct gctatctaac
     7801 aaacggagca caaaaataaa aataaaaatt aaatattaaa tataaagcac agttaatttt
     7861 gattaaaaag ttgactgtct tttcttttgt agaaatttaa gcataggtaa taaagaaatg
     7921 actgcattta agcattatgt atataattgg aggcaataaa aagccagcaa agcccataaa
     7981 atagagctgt gctagcaaca tgatgagtta taagaagata atcaaaaact tacgctttat
     8041 tttaatttaa aaaaatgatt actgtaaaga aatcattttt tattataatc actgatacat
     8101 caaatattct aaataaaatt tttatttatc tggaattgta tattttttct ataatattaa
     8161 ctacagcgta cattgcccaa gcacagatag ccaatacaaa tactcccatc attactaaat
     8221 ctaatttaaa tacttgagaa ccatatacta tcaagtagcc tattccatat cttgaaacta
     8281 aaaattctcc aactattaca cctacccatg ccataccaat attaatttta gttaaattta
     8341 ttaaatttcc tgtgtttgca ggtaatatga gtttaaataa tatttgagat ttagtagctc
     8401 caaagctttt taacatctta attttttctt catctatatt cataaaataa ttataagctg
     8461 aaagtatagt aacaacaaca gaaatcgtta cagctgttac gacgattcct tctattccag
     8521 cacctaccca aactataatt ataggagcta aagcagtctt aggaagagca tttaaaacta
     8581 ccaaaaatgg gtctaatatt tttgataatt tttctgacca ccaaagagca attgctacca
     8641 aaatacctaa tacagtacct ataacaagac caagaacagt ttcatataca gatattccaa
     8701 tatgtttaaa taattgacca ctaaatgcat attgaataaa taaattgtaa atatcacttg
     8761 gtttgctaaa tagaaatgta tttataacat ttaagtcagc taaaagttcc cagattacaa
     8821 taaaaccaat taaaattaat agctggtaga ataatacatt tcttttttct ctttttacac
     8881 ctttaacata gtttatatat ccttcagatc ttttattaag cctcatgtgt atctagctcc
     8941 ttccataata tgtcgaaata cttttgaaaa tcaggaactt tacgtgtttt gagaggcgtt
     9001 tttgttccat ccatgttaag ttcaatatcg taaatatttt ttatggtagc tggtctttta
     9061 gatagtattg caaccttatc tgatatgctt attgcttctg ctatagctaa aaaagtgcaa
     9121 ctaattgctt aaagaaacag aattatccac ttcaacaccc atataaacaa tattaagatt
     9181 tcctaaactg ctatcccaaa cgatactatt aataatagta gataataaca atctcttatc
     9241 atctaaactg gcattatcaa cttcatcatt aaatctcttc aaattactaa caataatatc
     9301 aatattaata ctgtcactac taatattgat gttactagat tccaaagtat taatcttatc
     9361 ctttaaaata ctaatctcat tatttaggct ctcaatctgg gaaactatat aatcagaagc
     9421 agtactacca gtattttttg caagctctat aactaaattt ttaataagtc tttccttatc
     9481 taaaatttga tttttaagta aattagtctc agatttaaaa atattaaaaa cattatcagt
     9541 cttaatgtta ttactattga atgcatttat aatagactct tctttacaat tcttaatttt
     9601 atctataact aaactttcaa ctaatggacc attaagattt ttacagttgc aactatcaga
     9661 cccaagtgtt tttttagtac cacaaatata ataatacttt aaatccttgt cagttttttt
     9721 gcctttatag gtaattctca tattagaacc acatttacca cattttataa gaccagataa
     9781 aagagcagtt tttccagttg atatccttgg agttttatca cgatttttat ctaattgatt
     9841 ttgtacttca agccatattt caggttctat gactccatgg tgttttgcaa ttgctgctat
     9901 tgagttagat gtatttttag cataggtaag gattccacat tttctatctg gagtacctac
     9961 aacatccata ccactatttt taaggtaatc taaaatttca tcattagctt ttacatatgc
    10021 tggatttcta agtattctag ataaagaact agggtcaaat ttaccaccac gaggacccct
    10081 tattccatta ttatagacat atttatataa cttagataag gacctaagtt ctaaatattt
    10141 attgtatatt agctttacaa tctccatttg ttcatcattt acttgaagct tatacatact
    10201 tctttctttt agattttcat ctaagaatgt gattttttca ctgttgaatc catgaggtgt
    10261 attgcctcca agccatctac cactacgggc taattcatac atattgtctt ttatacgttc
    10321 tgctattgtt tctctttcta attgagcaaa tacacttgat ataaacatca tagctcttcc
    10381 cattggagtt gaagtatcaa attgttcttt gatagatata aatgatatgt ttagttcatt
    10441 taatttttct attaaagtag agaagtcaga tacatttctc gatatcctat ctaatctata
    10501 acaaactagg taactaaatt tcttatcttt ggcatcctgc atcattttct taaattgtgg
    10561 tctatctata ttgctaccac taaatccctc atcttcatat atcaaaaaat cttttacacc
    10621 aatagacttt gcataatcaa tacataattg tatttgattt tctatagaat caccttttcc
    10681 agtaaatctt gattttcttg aataaattgc agctttcaaa atta
//
LOCUS       DBGJFF010000131        10466 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.133, whole genome shotgun sequence.
ACCESSION   DBGJFF010000131 DBGJFF010000000
VERSION     DBGJFF010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10466
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.133"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(<1..432)
                     /locus_tag="ACOIAF_003171"
     CDS             complement(<1..432)
                     /locus_tag="ACOIAF_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGZ2670168.1"
                     /translation="MRMIWNKGHRIRASDKNLVYRFSLGTLLIVFVVVLLLLNMKQLM
                     RTDWEHISLLENGLTLSPYNFITILIATGVCALVAFWYYRFCYDSFKKLLHRQKLARM
                     ILENKWYEADTVQDSGFFTDLQSRSREKIVWFPKIYYQMEKG"
     gene            complement(543..1049)
                     /locus_tag="ACOIAF_003172"
     CDS             complement(543..1049)
                     /locus_tag="ACOIAF_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcxB family protein"
                     /protein_id="HGZ2670169.1"
                     /translation="METVELRFQYTQSEYIKAERQYLISSKTIHKYDIALVAVFLLLS
                     VVYMLFSSFSVFSILIFGLIIVVTALGSYLYILMPILKFKQTAKYHEEYTLVFSKENI
                     KFKTQSIDSEVKWDIYSALWESHDFYYLIQAPRLYTLIPKRVFKDLNEKQLFEEIAQS
                     RVKTTKHI"
     gene            complement(1106..1648)
                     /locus_tag="ACOIAF_003173"
     CDS             complement(1106..1648)
                     /locus_tag="ACOIAF_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HGZ2670170.1"
                     /translation="MKGFERKNQLFSLCGLNCGLCPMLLGNYCGGCGNGNQSCRIAKC
                     SLEHGQIEYCYECKQYPCEKYQHIDDYDSFITHKRRKADLERAKNIGIEQYNLEQQEK
                     TQILSYLLSNYNDGRRKTFFCVAVNLLELSELQEAMKQIQSNDELTLLPFKAQCLYVV
                     EVFQKIAERRNIKLKLFKKK"
     gene            complement(1709..2311)
                     /locus_tag="ACOIAF_003174"
     CDS             complement(1709..2311)
                     /locus_tag="ACOIAF_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGZ2670171.1"
                     /translation="MDKVVIYIHGKGGNAEESSHYKPLFSDCDVVGLNYTAQYPWEAK
                     EEFPSLFDLICKHYKSVQIVANSIGAYFAMNALSDKQIEKAYFISPIVNMEKLIADMM
                     YWANVTEDELRDKKEIKTTFGETLSWEYLCYVKENPTTWKIPTNILFGEKDNLTSYAT
                     ISEFTKQTNATLTVMRNGEHWFHTEEQMKFLDEWINKSAR"
     gene            complement(2366..2779)
                     /locus_tag="ACOIAF_003175"
     CDS             complement(2366..2779)
                     /locus_tag="ACOIAF_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HGZ2670172.1"
                     /translation="MLDKIPNAEEMTALVGQSLYAIWNKLCALIDEKYDMECLWNKGG
                     KAWTYEYKYRRGGKTLCALYARENCVGFMIILGKDERLKFDTDRNSYSKEVQKIYDET
                     KTYHDGKWLMFEPTDTILFNDFVKLLGIKRKPNRK"
     gene            complement(2843..3301)
                     /locus_tag="ACOIAF_003176"
     CDS             complement(2843..3301)
                     /locus_tag="ACOIAF_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGZ2670173.1"
                     /translation="MKFKNPMLVVTDIDKSVEFYKKVFGLRVIMDFGANKTLTGGLAL
                     QTSETYKEFIGTSDISFGGKNFEVYFEEDDFDKFADRLKEYDIEYVHPIIEHSWGQRV
                     VRFYDPDKHIIEVGENMKIVCKRFLNSGMTPEQVAERMDVPMKFINACMR"
     gene            complement(3382..3795)
                     /locus_tag="ACOIAF_003177"
     CDS             complement(3382..3795)
                     /locus_tag="ACOIAF_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HGZ2670174.1"
                     /translation="MRLANGIVLDNEKTFGVLKFSALRHEVRVENEDGTTSDEIKRRT
                     YDLKCSVQERMIQVSIPADVPVKDFPYNAEVELINPVADTVANANFRGADVDWYIKAD
                     DIILKNKGNQPAGNPQNNNPQNLAPQGQQKNKLAN"
     gene            complement(3810..4133)
                     /locus_tag="ACOIAF_003178"
     CDS             complement(3810..4133)
                     /locus_tag="ACOIAF_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HGZ2670175.1"
                     /translation="MELKYIVPNVAQTFGNLEFAGENKVDQQRINGRYTPVSRSYNLY
                     SDIQRADDIIVVLPAKAGEKHFKPEQKVKLINPRITAEGYNVKGRGFTNYILMADDML
                     PAESK"
     gene            complement(4355..7402)
                     /locus_tag="ACOIAF_003179"
     CDS             complement(4355..7402)
                     /locus_tag="ACOIAF_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HGZ2670176.1"
                     /translation="MKKILKRLCTGFLAFATVVTALPSSTVHASNTQYWTESKERVGI
                     VEQVMNDGSISSTFNEGHLTVEGKDAYCIDINTAFKNGYKTRTDASSRMSADRISDVA
                     LSIEYVKQYTDSHSGISSKHAYLLRQLVVWQRLSVQLDWQCDNVRASYDEIPKAVQDE
                     VFAGAKAFVKENKGRYDCGGYIYSGEGQELGQFWAKLAVGNAKLQKTSTNANITDGNG
                     IYSIAGATYGVYSDKDCTKQLATLTTDTSGNTEAVEVRATTVYIKELSAPAGFKIDKT
                     VYSLSVEAGKTATLKVSDTPKVTDTLIELFKIDMEIQKSNPQGNASLEGAEFTWNFYA
                     GYYNKNNLPAQPTRTWVTKTIAEKDSDGAIHYITRLANAYKVSGDSFYMQDGKNVLPL
                     GTLTVEETKSPSGYLLEGAYMQGDGSEEQIKGMYLTQITEDGDLAVLSGSNQYHVSDK
                     VIRGGVKIQKRDLETGDTKAQGGATLKDTTFEIISLNDNAVLVEGKLYKKNEVVKTIY
                     TDIEGIASTSADLLPYGKFRMSEQKPPEGYLTEGAKEIDFEITENGKIVDLTDEAHSI
                     YNQIKRGDIEGVKIGAGSHKRLADVPFRITSKTTGESHVVVTDDNGQFSTASDWASHK
                     HNTNAGKTSEDGVWFGTSEPDDSKGALLYDTYIIEELRCESNKGFKLIPPFEIVISRN
                     NVVVDLGTLTDEYEKEITIHTTATSKDGEKTILAGNDVTIIDTVKLDGLVKGTKYQLK
                     GWQMLKEENAELLVGGKRVESDYTFVADDEAMKVEIAYTFNASALGGKNLVTFEELYD
                     LSNPEEPVKVAEHKDIEDDGQTVLITERIIKIHTTATDKDGKKEIEAGKDVTIVDTVK
                     LEGLEVGTKYQLVGWQMLKEENAELIINDKKVENDYTFTSDSETMEVKIAFTFDASAL
                     GGKQFVTFEELYDLSNPDKPIKVTEHKDIEDDGQTVTIKEVPETPTPEEPEKPTTPDT
                     PTKTDSPKTGDNTNILAFAIMMFVSAGGLAGTYFFKRRKMKKS"
     gene            7787..8650
                     /locus_tag="ACOIAF_003180"
     CDS             7787..8650
                     /locus_tag="ACOIAF_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HGZ2670177.1"
                     /translation="MKFFKVDTKAKVLRYVFVLTLTIMAVLAIKHDYKYNNLRKDASI
                     NLNSNKEAVTVMSNQSLPKGNTNSKQTLSVSTIGSKTIVKNDEYLESTINMPVITNSN
                     KIVERSTNDKIKNDIFEFYNKSYKEAKQYLKDNPDEKNKFVANVDFELKKNTDSALSI
                     KVRYYTYSGGAHGFYQDIAYNVDMRTGKFLELMDLFKDNTKYKEVIDEEIKRQIAELE
                     KKDEENIGIYNFKGIKENQNFYLQDENLVVYFDLYDITPYAAGIPEFSINKALISSML
                     KPEYVDLFDLK"
     gene            8717..9706
                     /locus_tag="ACOIAF_003181"
     CDS             8717..9706
                     /locus_tag="ACOIAF_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HGZ2670178.1"
                     /translation="MLLLKKDDIKKVFTMRDAIEADKEAFRIYCEGKSVNPLRTNISV
                     PSQDASMLFMPGYVEELGCGGIKIVSVFPKNAQKGKPVIPATVLLLDGETGEVSAVLD
                     GTYVTQIRTGAASGAAIDVLAKKDSKIGALIGLGGQGEPQLEAMIEARNLDEVRVFSR
                     NKESREKFAEEMNLKLSKYNTKIVAVESSDEAIDNADVIVLATPSKQPVLNGNLVKKG
                     ALISAVGSYMPDMQELCPNCLTRASKIFFESTDAVLSESGDILIPLKEGKISKDDFSG
                     DLGNVINGTIPGREDDDEIIVFKTVGIGVQDVVTAKRIYDKAKENGIGIDWKW"
     gene            9798..10409
                     /locus_tag="ACOIAF_003182"
     CDS             9798..10409
                     /locus_tag="ACOIAF_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HGZ2670179.1"
                     /translation="MKMVKKLILSTIMALVLVLTIGCSNAEEKVKSNFEANLAALEMY
                     KYDEKGISDALTEAEYKQIQTYNSKDKVSMIAQLTPLYGKENAEKIYETIVKLIQGVE
                     KTVTVESSSKDKITLKVVSKALDTSKVMSEANTLVKSYVTDEKNYDKKEKEVQDDMTK
                     MIIDLYNKKPTKEVTTMITYTKKDGKWQPPSNIQKLCEGIYTI"
BASE COUNT         3238 a         1884 c         1807 g         3537 t
ORIGIN      
        1 tcctttttcc atttgataat agatttttgg aaaccagacg attttttccc ttgatctgct
       61 ttgcaggtca gtaaaaaaac cgctgtcttg tacggtatcg gcttcatacc acttgttttc
      121 cagtatcatt cttgccagct tttgacggtg cagtagcttc ttgaaactgt catagcagaa
      181 gcggtaatac caaaaagcga ccaatgcaca aacaccagtc gctatcagta tggttatgaa
      241 gttgtaaggg gaaagcgtca agccgttctc taacaagctg atatgctccc aatcggtacg
      301 catgagctgt ttcatattca gtagcagaag aaccaccacg aatacaatta gaagcgttcc
      361 caatgaaaag cgatagacga ggttcttatc gctggctctg atacggtgtc ctttattcca
      421 aatcattcgc ataaatcaat cactccaatc ttaaatttaa gtatgaaaaa agcagtcaat
      481 cttaaaagac taaccgcttt gtgtgtatga atatgaagtt gttacattgt agggtatgtt
      541 cattatatat gctttgttgt tttaactctt gattgtgcaa tttcttcaaa tagctgtttt
      601 tcgtttaggt ctttaaaaac tcttttggga attaacgtat ataatcgtgg tgcttgaatt
      661 aagtaataaa aatcatggct ttcccatagt gcagagtaaa tgtcccactt cacttctgag
      721 tcaatgcttt gtgttttaaa tttaatgttt tccttagaaa ataccagcgt atattcctca
      781 tgatattttg cagtttgttt gaatttcagt ataggcatta ggatatataa atagcttccc
      841 aatgcagtta caacaattat caatccaaat attaaaatac taaatacgct aaaagaggag
      901 aacagcatat aaacgactga cagcaacaaa aatacagcga ctaaagcaat atcatattta
      961 tgtatagttt tactagaaat taaatattgc ctttctgctt ttatatattc actttgagta
     1021 tattgaaatc ttaactctac tgtttccaaa ggtattcccc cattcgtcat ataaataatc
     1081 tttgctacaa tttcaaattt gaaaatcact ttttcttgaa aagtttcagt tttatatttc
     1141 tcctttcagc aatcttctga aacacctcca ccacatacaa gcattgtgcc ttaaatggta
     1201 gtaaggtcaa ttcgtcattg gattgtattt gtttcattgc ttcttgtaat tctgaaagtt
     1261 ctaataaatt gactgccaca caaaagaaag tctttctacg cccgtcatta tagttagaaa
     1321 gtaagtaaga aagaatttgt gttttttcct gctgttcaag attatattgc tcaatgccta
     1381 tgttttttgc tctttctaaa tctgcttttc gccgtttatg cgtgatgaaa gaatcataat
     1441 catcaatgtg ttgatatttt tcgcatggat attgtttgca ttcatagcaa tattcaatct
     1501 gtccatgttc aagactacat tttgcaattc tgcatgattg attaccattg ccacaaccgc
     1561 cacagtaatt tcccaaaagc atagggcata gtccgcaatt caatccgcaa agagaaaata
     1621 attggttttt tcgttcaaat cctttcatag taccagtctc ctaacttcaa atttatcttt
     1681 gtatttattg acttttacaa actgggattt atctggcaga cttgtttatc cactcatcaa
     1741 gaaatttcat ttgttcttcc gtgtgaaacc aatgctctcc gtttctcata acagtaagtg
     1801 ttgcattagt ttgttttgta aattcagata tagttgcata agaagtcaag ttatctttct
     1861 caccaaataa aatatttgtt ggaattttcc atgtagttgg attttctttt acataacaga
     1921 gatattccca tgagagtgtt tctccaaaag ttgttttaat ttctttttta tctcgaagtt
     1981 catcttctgt aacatttgcc caatacatca tatctgcaat cagtttttcc atattcacaa
     2041 taggtgaaat aaaatatgct ttttctattt gcttatctga taaggcgttc atagcgaaat
     2101 aggcacctat gctatttgca actatttgaa cagatttgta atgtttgcaa atcaaatcaa
     2161 aaagtgatgg aaattcttct tttgcttccc atggatattg tgcagtatag ttgagtccaa
     2221 ccacatcaca atcactaaaa agaggtttgt aatgtgaaga ctcctctgca tttccacctt
     2281 taccatgtat ataaataact actttatcca taatagttgt cctcctcaac ttccgatttg
     2341 tatactaaat tgaagtataa aatcatcatt tcctgttggg ctttcttttt attcccaata
     2401 atttaacaaa atcattaaat aagattgtat ctgttggctc aaacatcaac cattttccgt
     2461 catgataggt ttttgtttca tcataaattt tttgcacttc ttttgaataa ctatttctat
     2521 ctgtatcaaa ttttaatcgt tcatctttcc ctaagataat cataaatcct acacagtttt
     2581 cccttgcgta caatgcacaa agcgtttttc cgccacgacg atatttatac tcataagtcc
     2641 acgctttacc acctttgttc cataaacatt ccatgtcata cttttcatca attaaggcac
     2701 acagtttatt ccatatagca tataatgatt gcccgactaa agcagtcatt tcttctgcat
     2761 taggtatttt atccagcata ctcatttctc ctttataact tcttatttat ctttgtattt
     2821 attgacttct acaaacaaga atttatctca tacaggcatt tataaatttc ataggcacgt
     2881 ccatgcgttc tgcaacttgt tctggtgtca ttccgctatt taagaaacgc ttgcatacta
     2941 ttttcatgtt ttctccaact tcgataatat gtttatctgg gtcataaaag cggactacac
     3001 gttgtcccca tgaatgttct ataatagggt ggacatattc aatatcatat tctttcagcc
     3061 tatcagcaaa tttatcaaaa tcatcttctt caaaatagac ttcaaaattt ttaccgccaa
     3121 aagaaatatc acttgtaccg ataaactctt tgtatgtttc acttgtctgt aaggctaagc
     3181 cacctgttaa agttttattt gctccaaaat ccataattac acgaagtcca aaaacttttt
     3241 tataaaactc aactgattta tctatgtcag tcactaccaa cataggattt ttaaatttca
     3301 ttttcgctac ctccgcaagt tcaaattatc tgtttccata cccatattat aacagtcact
     3361 tatatttgct acaatgaaaa tctagtttgc cagtttattt ttttgttgtc cttgtggtgc
     3421 aaggttctgt ggattgttgt tctgtggatt tcctgctggc tggttgcctt tgttcttcaa
     3481 gataatatcg tcagccttga tgtaccagtc cacatcagcc cctctaaagt tggcatttgc
     3541 caccgtatca gctacgggat tgattaactc cacttccgca ttataaggaa agtcctttac
     3601 gggaacatca gcggggattg acacttgtat catgcgttcc tgcacactac atttcaaatc
     3661 ataagtacga cgcttaattt catcacttgt cgttccgtcc tcattttcta cacgcacctc
     3721 atgtcgaagt gcagagaatt tcaatactcc aaaagttttt tcgttatcta atacaattcc
     3781 atttgctaat ctcataatca aataccccct tatttgcttt ctgctggtaa catatcatca
     3841 gccattaaaa tgtagtttgt aaatccacgt cctttgacgt tgtaaccctc tgcggtaatg
     3901 cgtgggttaa tcagttttac tttctgctct ggcttaaaat gcttctcgcc agcttttgca
     3961 ggcaatacaa caataatgtc gtcggctctc tgaatatccg aatacaagtt gtaacttctt
     4021 gatacgggag tgtaacgccc attgatacgt tgctggtcta ctttgttttc ccctgcaaat
     4081 tccaagtttc caaaagtctg tgccacattc ggcacgatat attttaattc catattgttt
     4141 tacctcgttt ctttctatta gtttgattgt tttgtattct ggcagttaat gagataccag
     4201 caagcccaga cagtaaagag taaactgtat gctgacgcac ccttgacgat ctgggttctt
     4261 gcaggtaatc ggcagacaag ccagaataga agtagtctgc ctatttaagt tacggcggag
     4321 agcgtgattt ttcttttctc atgcagttac cgccttatga tttcttcatc ttacggcgtt
     4381 tgaagaagta tgtaccagcc agaccgccag cagaaacaaa catcataatc gcaaaggcaa
     4441 gaatatttgt attgtcgccc gtttttggac tgtctgtttt cgttggtgta tctggtgtag
     4501 ttggcttctc tggttcttct ggcgtaggtg tttctggtac ttccttaata gtaaccgtct
     4561 gcccgtcgtc ctcaatgtcc ttatgctccg taacttttat cggtttgtct ggattgctta
     4621 aatcgtataa ttcctcaaaa gttacaaact gtttaccgcc aagagcagaa gcgtcaaaag
     4681 taaatgcaat ctttacttcc atagtttcac tgtcagacgt aaatgtgtag tcattttcaa
     4741 cttttttatc attgatgata agctctgcat tttcttcttt taacatctgc cagcccacaa
     4801 gctgatactt tgtaccgact tctaagccct ctaacttgac cgtatcaaca atcgttacgt
     4861 ccttgccagc ttcaatctct ttcttgccgt ccttgtccgt agctgtggta tggattttga
     4921 tgatacgttc tgtgataagt accgtttgtc cgtcgtcctc aatatcttta tgttccgcaa
     4981 ctttcacggg ttcttctgga ttgcttaaat catacaattc ttcaaaagtt acaaggttct
     5041 taccgccaag agcagacgca ttgaatgtat aagcgatttc cactttcata gcttcatcat
     5101 cagcgacaaa ggtataatca ctttccactc gtttaccacc tacaagaagc tcggcatttt
     5161 cttctttcaa catctgccag ccttttaact gatactttgt accttttaca agaccgtcta
     5221 atttgaccgt atcaatgata gtaacgtcat tccctgcaag gatagtcttt tcgccgtcct
     5281 tgctggtagc tgtggtatgg atagtgattt ctttttcgta ttcatcagtc agcgtaccta
     5341 aatcaacgac aacattattt ctggaaataa caatctcaaa cggtggaata agtttgaagc
     5401 ctttgttgct ttcacaacgt aattcttcaa tgatgtaggt atcataaagc aaagcacctt
     5461 tgctgtcgtc tggttcagaa gtaccaaacc acacaccgtc ctcgcttgtt tttcctgcat
     5521 tggtattgtg cttgtgggaa gcccaatcag aagcagtaga gaattgtccg ttatcatcag
     5581 ttacaacaac atgactttcg cccgtcgtct tgcttgtgat cctaaacgga acatctgcaa
     5641 gacgcttgtg cgaacctgca ccaattttta cgccctcaat atcgccacgt ttaatctggt
     5701 tatagataga gtgggcttcg tcagtcaaat ctacgatttt tccattttct gtgatttcaa
     5761 agtcaatctc ttttgcaccc tctgtcaaat atccctctgg cggtttttgt tcagacatcc
     5821 taaattttcc gtaaggtaac aaatcagcag aagtagaagc gataccctca atgtcggtat
     5881 agattgtctt tactacttca tttttcttgt atagcttgcc ctcaaccaag acagcattat
     5941 catttaagga aatgatttca aaggtggtat ctttcaaagt cgcaccgcct tgagctttcg
     6001 tgtcacccgt ttctaaatct cgtttttgga ttttcacacc gccacgaatg actttatctg
     6061 atacatggta ctgattactt ccagacaata cggcaaggtc gccgtcctct gtaatctgtg
     6121 tcagatacat tccttttatc tgttcttcgc taccgtctcc ctgcatatat gcaccctcta
     6181 ataagtaacc agacggactt tttgtttctt cgacggttag tgttcctaaa ggaagcacgt
     6241 ttttaccgtc ctgcatatag aagctgtccc cagatacttt ataagcgtta gctaaacggg
     6301 taatgtaatg gatagctccg tcgctgtctt tttcagcgat agtctttgtt acccatgttc
     6361 ttgtaggctg tgctggaaga ttgttcttgt tgtaatatcc agcgtagaag ttccatgtaa
     6421 actctgctcc ctctaaagaa gcgtttccct gcggattgga tttctgtatt tccatgtcaa
     6481 tcttgaatag ctcaatcaag gtatctgtta cttttggtgt atcagatact tttaaagtcg
     6541 ctgtttttcc agcttccaca gataaagagt aaactgtttt atcaatttta aatcctgctg
     6601 gtgcagaaag ttccttgata tatactgtag tggctcttac ttcaacggct tctgtatttc
     6661 cgctggtatc agtcgtaagg gtggcaagct gtttcgtgca gtctttatca gaatacacac
     6721 cgtatgttgc accagcgatt gaataaatcc cgttgccgtc tgtaatattg gcattggtgg
     6781 aagtcttttg aagtttggca tttccgacag ctaacttcgc ccagaattgc cctaactctt
     6841 gaccctcgcc agaatagata tatccgccac agtcataacg tcctttgttt tctttcacaa
     6901 aggcttttgc tcctgcgaat acttcatctt gtacggcttt tgggatttca tcataggaag
     6961 ctcgcacatt atcgcattgc cagtcaagct gaacacttaa tctctgccag acaacaagct
     7021 gtcttaatag gtaggcgtgt ttactgctta ttccgctgtg gctgtctgtg tactgtttca
     7081 catactcaat agataaggca acgtcgctta tccggtcggc actcatgcgt gagcttgcgt
     7141 ctgttctggt cttatagccg tttttaaacg ctgtgttgat gtcaatacaa taagcgtcct
     7201 tgccctcaac ggttaaatgt ccctcattaa aggtagaact gatagaaccg tcattcatta
     7261 cttgttctac gataccgaca cgctctttac tttccgtcca atactgtgta ttagacgcat
     7321 gaaccgttga actcggtaaa gcggtaacga cagttgcaaa ggcaagaaag cctgtacaca
     7381 atcgttttaa tatctttttc ataattctaa atgctccttt cattttggta gtaaaatagc
     7441 cgtccactag gaacggctgg aaatagaaaa ggaacgctga tattgtgcgt tccgtagtct
     7501 atgaagtatt caatttttct tgtttcaata ctgatgtgct gtaccgtatc tttgcatatc
     7561 tgggaaatag gacgtatcaa ggctcattca atcgtagtaa attagtagta ttttgatgtg
     7621 tttagctcct tgataatgcc gatagataca gcgttttagc ggataatcaa atttttagtg
     7681 tgtttttata aataaaaatt aatcttttat taaattatat ggataaaaaa ccctagaaac
     7741 tccaaatata ataagagaga tttatattta ggaggaaaat gataatatga aattttttaa
     7801 agtcgataca aaagctaaag ttttaagata tgtatttgtt ttaacattga ctataatggc
     7861 tgttttggca attaaacatg actataaata taataattta aggaaagatg cttcaattaa
     7921 tttaaatagt aataaggaag cagtgactgt tatgagtaac cagtcattac caaagggtaa
     7981 tacaaatagt aaacaaactt tatcagtaag tacaatagga agtaaaacaa tagtgaaaaa
     8041 tgatgagtat ttagaaagta ctataaacat gcctgtaata acaaattcaa ataaaattgt
     8101 tgaaaggtct acaaatgata agataaaaaa tgacatattt gaattttata ataaatcata
     8161 taaagaagca aagcagtatc ttaaggataa tcctgatgag aaaaataagt ttgttgcaaa
     8221 tgtagacttt gaacttaaaa agaatactga ttctgctctt agtataaaag tgagatatta
     8281 tacttatagt ggtggagcac atggatttta tcaagatata gcatataatg tggatatgag
     8341 aactggaaaa tttttagaat taatggactt gtttaaggat aatactaaat ataaagaagt
     8401 tatagatgaa gaaattaaaa gacaaatagc tgaacttgaa aaaaaggatg aagaaaatat
     8461 aggaatatat aattttaaag gtataaaaga gaatcaaaac ttttacttgc aagatgagaa
     8521 tctagttgta tattttgatt tatatgatat aactccatat gcagcaggga taccagaatt
     8581 ttcaataaat aaagcgttga taagttctat gctaaaacca gagtatgtag atttatttga
     8641 tttgaaatag aagattaaat taaatgttat gctataatat ttagcataga gacttattaa
     8701 aattgggggt ataaaaatgc tacttttaaa aaaagatgac ataaaaaaag tatttacaat
     8761 gagagatgca attgaagctg ataaggaagc ttttagaatt tattgtgaag gaaagagtgt
     8821 aaatcctttg agaaccaata tatctgttcc aagtcaagat gctagtatgt tatttatgcc
     8881 aggatatgta gaagaattag gctgtggggg aataaaaatt gtatctgtat ttcctaaaaa
     8941 tgctcaaaaa ggcaaaccag taattccagc aacagtacta ttattagatg gagaaactgg
     9001 agaagttagt gcagtattgg atggtactta tgtaactcag attcgtactg gagcagcctc
     9061 tggagcagct atagatgtat tggctaaaaa agattctaag attggagctt tgataggtct
     9121 aggaggacaa ggtgagccac aacttgaagc catgatagaa gctagaaatt tggatgaggt
     9181 aagagttttt agtagaaaca aggaaagtag agaaaaattt gcagaagaaa tgaacttaaa
     9241 attaagtaaa tataatacta aaatagttgc agttgaatca tctgatgaag ccatagataa
     9301 cgcagatgtg atagttttag ctactccatc aaaacaacca gtacttaatg gaaatttagt
     9361 taaaaaagga gctttaataa gtgcagtagg ttcatatatg ccagatatgc aagagttgtg
     9421 cccaaactgt ttaactagag cgtctaagat attttttgaa tcaacagatg cagttttatc
     9481 tgagtcagga gatatattaa ttccattaaa agaaggtaag ataagtaaag atgattttag
     9541 tggtgattta ggaaatgtta taaacggaac tattcctgga agagaagatg atgatgagat
     9601 aattgtattt aaaactgttg ggataggtgt acaagatgtc gtaacagcaa aaagaatata
     9661 cgataaagca aaagaaaacg gtattggaat agactggaag tggtaaaata ttcaaaataa
     9721 ttctatagtt agacaaattt atgttataat gttgataaat aaatttttta ttattttaat
     9781 tgagggagga tgtttgtatg aaaatggtaa aaaagttaat attatctacg attatggctc
     9841 tagttttagt tctaactata gggtgttcta atgcagaaga aaaagtaaag tcgaattttg
     9901 aagctaattt agctgcatta gaaatgtata agtatgatga aaaaggaatt tctgatgcat
     9961 taacagaagc tgaatataaa cagattcaaa cttataacag caaggacaag gtttcaatga
    10021 ttgctcaatt gacacctctt tatggtaaag aaaatgctga aaagatatac gaaacaattg
    10081 ttaaattaat tcaaggtgtt gaaaagacag taacagtgga atcttctagt aaggataaga
    10141 taactttgaa agttgtaagt aaagctttag atactagtaa agtaatgagt gaagcgaaca
    10201 ctttagtaaa atcttatgta actgatgaaa agaattacga taaaaaagaa aaagaagttc
    10261 aagatgatat gacaaaaatg attatagacc tttacaataa gaaaccaact aaggaagtta
    10321 caactatgat tacatatact aaaaaagatg gtaaatggca accacctagt aatatacaaa
    10381 agttatgtga aggaatatat acaatttaat ttattttaaa gacacaaaac tatcttttaa
    10441 gcatataata aaatgtttgg aagata
//
LOCUS       DBGJFF010000132        10450 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.134, whole genome shotgun sequence.
ACCESSION   DBGJFF010000132 DBGJFF010000000
VERSION     DBGJFF010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10450
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.134"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            <1..1004
                     /locus_tag="ACOIAF_003183"
     CDS             <1..1004
                     /locus_tag="ACOIAF_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HGZ2670180.1"
                     /translation="NDNKFILISYFLELILIFILCNYTKTFNSIYFVPLILDISFLIK
                     EKYKYILFSFVIISSLIISLDKNIYLALESSSILLIITVLSIYIENENLSKLYSQNLY
                     DKLRISKDELKKVNADLETYASSIEELAILKERNRISREIHDSVGHSLSTTIIQLNAI
                     EKLLKDKPLIYDLVHELREFVTESFQDVRRAISELKPVEYENYQSLFKIKELVKSFIK
                     LTNINVKLTISKNTWNLSRNQSIALYRLIQESLSNSSRHGKATEIRIFITFNTSNLII
                     TISDNGIGCGNIKKGNGLNSINERIYELNGKVEFDNSNNGFTIRASFPKFSGGDFFE"
     gene            997..1644
                     /locus_tag="ACOIAF_003184"
     CDS             997..1644
                     /locus_tag="ACOIAF_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HGZ2670181.1"
                     /translation="MNKIKVLIVDDEKLIRKGLKIILSSYNDLEIVGDASNGYEALEF
                     CKMNDVDIVLMDIRMKVCDGVLGTRLIKEYNNSITLLILTTFNDDEYIKDAMKFGASG
                     YLLKDSSDEVLHEGIRSSFFGNIVLDKSVAEKIMTSEKTVKQEHLYDMYNLTEKEISI
                     IRLIANGLNNKEISQELFLSEGTIKNNITNILAKLELRDRTQLAIFAFKNKIVIE"
     gene            1772..2707
                     /locus_tag="ACOIAF_003185"
     CDS             1772..2707
                     /locus_tag="ACOIAF_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGZ2670182.1"
                     /translation="MDIVRVNNITKRFNDKLVLDNISFSVKKGEIFGLIGPNGAGKST
                     LINIITNLMLPNSGSIQINGLDLSKDYIKAKSIIGLVPQELAIIETLTPFDNLEYFGA
                     FYGLKGKLLKERIIEALEVTGLTEVKKKKVKKLSGGMQRRLNIGIALLNHPKILILDE
                     PTVGVDPQSRNHIFNFIKDISKKHETTVIYTSHYMEEVEHLCSKIFIMDEGKEIAFGD
                     NDYLKSLVSTNTKLIIEIKNINAQLIFDLKNTKGVISVLENNSLLELDIDKKLQLTDI
                     LSVIDKNNSKIMKISYEEPSLEDVFLNLTGKNLRD"
     gene            2721..3818
                     /locus_tag="ACOIAF_003186"
     CDS             2721..3818
                     /locus_tag="ACOIAF_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2670183.1"
                     /translation="MKLYMSVKTMLKSLKSSFILNLIYFLALPLILSWFLGMVTESMF
                     QNPIKTESTPIVIYDKDNTRLSNDLTKYLKNDLSYILTVKKDDSKAELKLTIPKGYES
                     SLLNEKSNTLNIEKLGTRDDIAILLQDILDTYHEKFYLNNSQKISSEDFSKLFNKNSI
                     DTSIINNNVKQSSYEYFALVSLGFLVIIFIMNNILSNYISESKGLSKRLYSMPITRVQ
                     FLIYDFVGLWIYSFIFLLLYVLFFRILGITFKGNFAILLLLCALSSYFMTSISTFVTS
                     FFSKKYSTAIVYALLFLQTIFGGIFSMISDTFTKLTSLSPTYLIGELFSNYETFKTID
                     SIGDLIFTCLIISTILIALAIVKEKYKWREV"
     gene            3821..4885
                     /locus_tag="ACOIAF_003187"
     CDS             3821..4885
                     /locus_tag="ACOIAF_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2670184.1"
                     /translation="MIRLTIANIKRYLKNHTLLINMVMLPIILIFSLNFFINNSGNQS
                     MYFSPVAIVSDSSGKYEHKLINSSKLKENSFRLNEQDKAMNLLKNNKVSAVFVLDKNF
                     SNSIDKLQRPVVKCFKIENGGGSLWAESQIESFITKSLKLKVDKNIDDKLTKTNIIDN
                     KPDKNKGSFLVVFLICYFMYINAAHLASDLFTLKKSNVLKRLISTDNKDIKIIFSIFL
                     GLFFIQSIVYTFALLCFSIIEDFNLSLNVLMIVLANSFVSTGFVFWVARVFKNESSIS
                     LISTFYSLIGLAISISSLIPSMDKLSFMTNLSKFTPFYWTIDAIKNNGSILINIIALI
                     LIGIIFVTAGSLKLRDFAKN"
     gene            5129..5626
                     /locus_tag="ACOIAF_003188"
     CDS             5129..5626
                     /locus_tag="ACOIAF_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HGZ2670185.1"
                     /translation="MYKIGESVMYPKEGACSVNDIVTKKINHEMQKYYELSVIFNSNL
                     KISIPVLNADRIGIRPVMDGNDVDNFIQSINKTDGVWIFDRKERLKLYQDKFHSGDVF
                     EIVKLIKMLMIQDSSKQLCSTDKEFLNKAQKFALSELAAAQCKSYTMVLEEMKKHILN
                     SKNIN"
     gene            complement(5803..6051)
                     /locus_tag="ACOIAF_003189"
     CDS             complement(5803..6051)
                     /locus_tag="ACOIAF_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670186.1"
                     /translation="MSRKAYEEALVELEKFIDERKEIIKSAEDCIDKYIVDRTLPFDY
                     KDKCVEWQQELLDIAEAQVLEANELSVLLQEKKELEED"
     gene            complement(6117..7133)
                     /locus_tag="ACOIAF_003190"
     CDS             complement(6117..7133)
                     /locus_tag="ACOIAF_003190"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HGZ2670187.1"
                     /translation="MEYNELLKSARENLNGSCKVCKVCNGVACAGEVPGMGGKGSGSS
                     FIENSKSLEKVKVNMRVIHDVSNPDTSIEMFGKKMSAPIFAAPVTGTTLNMGGKINER
                     DYIEPVVAGCANSGIYAMVGDTAVDAFLMENLDVVKKYNGAGIVFIKPWDNENIIKKI
                     RLAEEAGAFAVGVDIDACGLVTLSLHGKPVLPKNVKQIKELVKSTKLPFILKGIMTVE
                     DALMAVEAGVDAIVVSNHGGRVLDCTPGACEVLPKIADAVKGKVTILADGGVRTGLDV
                     LKMIGLGADAVLIGRPFVTASFGGATDGVETYVNKLQSELSSSMILTGCQTIKDIDGK
                     VIYK"
     gene            complement(7191..7958)
                     /locus_tag="ACOIAF_003191"
     CDS             complement(7191..7958)
                     /locus_tag="ACOIAF_003191"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HGZ2670188.1"
                     /translation="MQDKSVREIKEIIETLEVEKYMEYIELLRVDERKSVQGLAIKLA
                     KKLDNIRKEEERLETINIFENEGYDKGYLYIGGIDEAGRGPLAGPVVASVVVFKKDTK
                     IEGVNDSKKLSEAKRDELFEVIKEEALDYGIGIVNNEEIDEFNILNATYMAMKKAINC
                     LKKAPDYLLVDAATIPGIDISQNPIVKGDSKSISIAAASILAKVTRDSIMYQYDRVYP
                     EYGFKSHKGYGTKEHYEAIEKYGITPIHRKSFLKNIL"
     gene            complement(8026..10215)
                     /locus_tag="ACOIAF_003192"
     CDS             complement(8026..10215)
                     /locus_tag="ACOIAF_003192"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HGZ2670189.1"
                     /translation="MKYIIKRDGTKELFSKNKIEDAVYKASINSEGGVDRELAYDISR
                     KIANDYNSMEKCLSVEEIQDIVEVLLMESNRKDIAKHYILYRERRSEIRTKAWEFDEL
                     QKSIWKNKYRHNGESFDEWIKRISNNSSRISKLIRQKRFLFAGRILANRGLQKEGKKV
                     TYSNCYVIEPPKDSIEDIFDTAKKLARTFSYGGGVGIDISKLRPKGAKVNNAAKHTTG
                     AVSFMELYSMTTGLIGQRGRRGALMISMDVNHPEIEDFIDIKTDLEKVTKANISVRVT
                     DEFMQAVREKKLYNCTFEVETTGEKIVKIVNAYDLFNKFVKNNWDYAEPGILFWDKIK
                     TYNFMSEDKNFCFSGVNPCAEEPLPSGGSCLLGSINLSEFIIQPFGEHATFDINKLKS
                     CVREAVVALNDVLDEGLNLHPLQEQRDSVHKYRQIGLGVMGIADMLIKMGIRYGSDES
                     LELCDLIGKTMLNEAVKQSALIAKEYGTFSEYKYENISKSKFFIENLNEDTKEMVKKY
                     GLRNSQLLTIPPTGSISTMIGISGGIEPIFNLSYIRKTESLHDEDVYYKVYTPIVKEY
                     MDKKGITDEECLPDFFVTAMNIGYEERINMQKVWQKYIDASISSTINIPFETTVEDVF
                     NIYIKAWEAGLKGVTIFRDGCKRSGILINEKKEENNKEDKKENSKKEYYKNNKKEKDN
                     KDEKAKEIINKNFEEDEKFVCPECGSEGIAHTGGCSICLKCGYSGCN"
BASE COUNT         3642 a         1597 c         1199 g         4012 t
ORIGIN      
        1 caaatgataa taaatttata cttatttctt actttttaga acttatatta atttttatac
       61 tttgtaatta tacaaaaact tttaattcga tttactttgt tcctttaata ttagatatat
      121 catttttaat aaaggaaaaa tataagtaca tattgttctc ttttgttata atatcttctc
      181 ttattattag tttagataaa aatatatatt tggcactgga gtcaagctct atattgctaa
      241 ttataactgt actttctata tatatagaaa atgagaattt atcaaaatta tacagtcaaa
      301 atctttatga taagcttaga atttctaaag atgaattaaa aaaagttaat gctgatttgg
      361 aaacttatgc aagttctata gaagaattag caattttaaa agaaagaaat cgaatatcaa
      421 gagaaataca tgatagtgta ggacattctc tttctacaac aataatacaa ttaaatgcta
      481 tagaaaaact cttaaaagac aaacctctaa tttatgactt agtacatgaa cttagagaat
      541 ttgtaactga aagttttcaa gatgtaagaa gggctatatc tgagttaaaa cctgttgaat
      601 atgaaaatta ccaaagttta tttaaaatca aagaattagt taaaagcttt attaagctta
      661 caaatattaa tgttaaatta actatttcaa aaaatacttg gaacttatcc agaaatcaat
      721 ctatagcttt atatagactc atacaggaat ccctttccaa ctcatctagg catggaaagg
      781 caactgaaat cagaattttt attacattca atacttcaaa tcttataatt actattagtg
      841 ataatggtat aggatgtggc aatataaaaa aaggaaatgg acttaatagt atcaatgaga
      901 gaatatatga attaaatggt aaagttgaat ttgataattc taataatggt ttcacaatta
      961 gagcttcttt tcctaaattt tcagggggtg atttttttga ataaaattaa agttcttata
     1021 gtagatgacg aaaaattaat tagaaaaggt cttaaaatta tattatcatc ttataatgat
     1081 ttagaaattg taggagatgc ttctaatgga tatgaagctt tagaattttg taaaatgaat
     1141 gatgtagaca tagttcttat ggatatacga atgaaagtat gtgatggggt tctaggtaca
     1201 aggcttataa aggaatataa taattctata acattattaa ttttaacaac ttttaatgat
     1261 gatgaatata taaaagatgc tatgaagttt ggtgcttcag gatacctttt aaaagatagc
     1321 tctgatgaag tattgcatga agggattcgt tcttcatttt ttggcaatat tgtgcttgat
     1381 aaaagtgtag ctgaaaaaat catgacctct gaaaaaacgg ttaaacaaga gcatctttat
     1441 gacatgtaca atttaacaga aaaagaaata tctataatta gactaattgc aaatggactt
     1501 aataataaag aaataagtca agaactattc ttatctgaag gtacaataaa gaataacata
     1561 actaatattt tagctaaact agaacttcgt gacagaactc aactagcaat ctttgctttc
     1621 aaaaataaaa ttgtaataga ataaatctaa aaatataaag tgacttaagt cactttttta
     1681 gtgacttata gtaatagaat tataattcac aattctttat tataatatta tagcttttta
     1741 acttatacaa aattattttg gagggattac tatggatata gtcagagtaa ataatataac
     1801 aaaaagattt aatgataaat tagtacttga taacatcagc ttttctgtta aaaaaggtga
     1861 gatatttgga cttattggcc caaatggtgc aggtaaatct acacttataa atataattac
     1921 aaatcttatg ttaccaaata gtggtagtat tcaaatcaat ggtttagatt tatcaaaaga
     1981 ttatataaaa gcaaagtcta taatagggtt agttccacaa gaattagcaa ttatagaaac
     2041 tcttactcct tttgataact tagagtactt tggagctttc tacggtttaa agggaaaact
     2101 acttaaagaa agaatcattg aagcattaga agttacaggt cttactgaag tcaaaaagaa
     2161 aaaggtcaaa aaattatctg gtggtatgca aaggagatta aatattggaa tagcactctt
     2221 aaatcatcct aaaatattaa tattggatga accaactgta ggtgtagacc cccaatcaag
     2281 aaatcatatt ttcaatttta taaaagatat atctaaaaaa catgaaacta ctgtaatata
     2341 tacttcccat tatatggaag aagttgaaca cctttgttct aaaattttta ttatggatga
     2401 aggtaaagaa attgcttttg gagataatga ttatttaaaa tcactcgtat ctacaaatac
     2461 aaaattaatt atagaaataa aaaatataaa tgcccaactt atctttgatt taaaaaatac
     2521 taaaggtgtt atatctgttt tagaaaataa ttcattatta gaattggata tagataaaaa
     2581 attacaactt acagatatac tctctgttat tgacaaaaac aattcaaaaa taatgaaaat
     2641 ttcttatgaa gaacctagcc tagaagacgt atttttaaat ctaacaggga aaaatttaag
     2701 ggactaatgg aggtgatttt atgaaattat acatgagtgt aaaaactatg ctaaagagtt
     2761 taaaaagctc ctttatactt aatttaatat attttttagc tttgcctctg attttatctt
     2821 ggtttcttgg gatggttaca gaaagcatgt ttcaaaaccc tataaaaaca gaatctaccc
     2881 ccattgttat atatgacaaa gataacacaa gattatcaaa tgatttgaca aagtatttga
     2941 aaaatgattt atcttatata ttaacagtta aaaaagatga ttctaaggca gaattaaagc
     3001 ttactatacc taaaggctat gagagtagtt tattaaatga aaaatcaaat actctaaaca
     3061 tagaaaaact tggaactcgt gatgatattg caatattgct tcaagatata ttagatactt
     3121 accatgaaaa attttattta aataattctc agaaaataag ttcagaagat ttttctaaat
     3181 tatttaataa aaattctatt gatacttcta taataaataa caatgttaaa cagagctctt
     3241 atgaatactt tgctttagtt tcactaggat ttcttgtaat aatttttata atgaacaata
     3301 ttttgtcaaa ttatataagt gaatcaaagg gactttcaaa aagactttat tctatgccta
     3361 taactagagt acaattctta atttatgact ttgtaggttt atggatttat tcatttatat
     3421 tcttattatt atatgtttta ttctttagaa tacttggaat aacatttaaa ggtaattttg
     3481 ccatattgct cctactttgt gctttatcat cttattttat gacttcaata tctacctttg
     3541 taaccagctt ttttagtaaa aaatacagta cagcaatagt ttatgcatta ctctttttac
     3601 agacaatttt tggaggtatc ttcagtatga taagtgatac atttactaag cttacaagtt
     3661 tatctcctac ctatttaata ggagaattgt ttagtaatta tgaaacattt aaaaccatag
     3721 attcaatagg agatttaatt ttcacatgtt taattatctc tacaatatta attgcacttg
     3781 ctattgtaaa ggaaaaatat aaatggaggg aggtttaata atgattagat taactattgc
     3841 aaatataaaa agatatctaa aaaatcatac tcttttaatt aatatggtta tgcttcctat
     3901 tatactaatt ttttcactta atttttttat caacaactcg ggaaatcaat caatgtactt
     3961 ttctccagta gcaattgtat ctgactcttc aggaaagtat gaacataagt taataaatag
     4021 ttctaaatta aaagaaaatt catttagatt aaatgaacaa gataaggcta tgaatctatt
     4081 aaaaaataat aaggtttctg ctgtattcgt tttagataaa aatttttcaa atagtataga
     4141 taaattacaa cgacctgtag ttaaatgctt taaaattgaa aatggtggtg gtagtctttg
     4201 ggcagaatca caaatagaat cttttattac taaatcgtta aaattgaaag tggataaaaa
     4261 tatagatgat aaattgacaa agacaaatat aattgataac aaaccagata agaataaagg
     4321 ttcattttta gtagtcttct tgatatgcta ttttatgtat attaatgcgg cacatcttgc
     4381 ttctgattta tttacattaa aaaaatcaaa tgtgctaaaa agacttatct ctacagataa
     4441 taaagatata aaaataattt tcagtatatt tttaggacta ttttttattc aatcaattgt
     4501 atacactttt gctctattat gtttttcaat tatagaagac tttaatttga gtctgaatgt
     4561 attgatgatt gtactagcaa atagttttgt ttccactgga tttgtatttt gggtagctag
     4621 agtatttaaa aatgaaagtt ctataagtct tatatctaca ttttattctt taataggtct
     4681 agctatatct atctctagtc taataccatc aatggataaa ttatccttta tgactaatct
     4741 ttcaaagttc acaccatttt attggactat agatgctatt aaaaacaatg gtagtatttt
     4801 aataaatata atagctctaa tattaattgg aattatattt gtaactgcag gaagtcttaa
     4861 actaagagat tttgctaaaa actaataatt attaaatata attagtatct atttttatat
     4921 aattgtaaaa gaatatttca acatattaaa ttattttatc ttatattttt ctctttagtt
     4981 tcacacatca ccaagtctaa actttatatt atatattaat atttttgact tgtcaagttt
     5041 tttatgttat aatattaatc attagataat taaagcattg tacctaagtt gcaatatcta
     5101 aaattcaatt tggaggtata actagcatat gtataaaatt ggtgaaagtg taatgtatcc
     5161 aaaagaggga gcctgctctg taaatgatat tgtaacaaag aaaataaatc atgagatgca
     5221 aaaatactat gaattaagtg tcatttttaa tagtaacctt aaaatttcga ttccagttct
     5281 aaatgcagat agaattggca tacgtccagt tatggatgga aatgatgttg ataattttat
     5341 ccaatctatt aataaaacag atggtgtatg gatttttgat agaaaagaaa gacttaaact
     5401 gtaccaagat aaatttcatt caggagatgt ttttgaaatt gtaaaattaa tcaaaatgct
     5461 tatgatacaa gacagttcaa aacaattgtg tagcacagat aaggagtttt taaataaagc
     5521 tcaaaaattt gctttatcag aattagctgc tgctcaatgt aaatcctata caatggtatt
     5581 agaggaaatg aaaaaacata tattaaattc aaagaatata aattaaacca gttttttaaa
     5641 ctggttttat tttttatact aatcatccat gctactttag cttattttaa actgcctaat
     5701 ctaatattaa taaagttatc tcaattagat agatgtattt tattcaaata aaaaaaaact
     5761 aagagtaaat catattactg atttactctt agctaataaa atttaatctt cttcaagttc
     5821 ttttttctct tgtaataata cacttaattc atttgcttct aaaacttgtg cctctgcaat
     5881 atcaagtaac tcttgttgcc attccacaca tttatctttg taatcaaaag gtagcgttct
     5941 gtcaactatg tacttatcaa tacagtcctc cgcactttta attatttctt ttctttcatc
     6001 aataaatttt tctaattcta ctaaggcttc ttcataagct tttctactca tataaattcc
     6061 ccctttgtac aattattcta cattaaaatg atttttcctc ttttttgtat aaaattttat
     6121 ttgtatataa cttttccatc aatatctttt atagtttgac atccagttaa tatcatagaa
     6181 ctacttagct ctgattgaag tttatttaca taagtttcta caccatcagt tgcaccacca
     6241 aatgaagcag tcacaaaagg tcttcctatt aatactgcat ctgcacctaa acctatcatt
     6301 tttagtacgt ctaaaccagt tctcactcca ccatctgcaa gtattgtaac tttaccttta
     6361 acagcatcag caatttttgg taaaacttca catgctcctg gtgtacagtc aagcactctt
     6421 cctccatgat ttgatacaac tattgcatcc acacctgctt ctactgccat taaagcatct
     6481 tcaacagtca ttataccttt caatataaat ggtagtttag tagactttac taactccttt
     6541 atttgtttaa catttttagg aagtactggt tttccatgta atgataaagt tactagacca
     6601 caggcatcta tatcaactcc aactgcaaat gcaccagctt cttcagctaa tcttatcttt
     6661 tttataatat tttcattatc ccaaggttta ataaatacaa ttccagctcc attatacttt
     6721 ttgactacat ctaaattttc cattaaaaaa gcatctactg ccgtatcacc aaccattgca
     6781 taaataccac tattagcaca tcctgcaact actggttcta tgtaatctct ttcattaatt
     6841 tttccaccca tatttaaagt agttcctgtt actggagctg caaatatagg tgcactcatt
     6901 ttctttccaa acatctctat agatgtatca ggatttgata catcatgtat tactctcata
     6961 tttactttta ctttttctaa actcttgcta ttttctataa aagaagaacc acttccttta
     7021 cctcccatgc caggcacttc acctgcacaa gcaaccccat tacaaacctt acaaacttta
     7081 caacttccat ttaaattttc tctagcactt tttaataatt cattatattc catctttaat
     7141 cccccattaa ctatagtaat ttatattata tacattatat catattatat ttacagtata
     7201 tttttcaaaa aactttttct gtggatagga gttatgccat atttttcaat agcttcatag
     7261 tgttctttag ttccatatcc tttgtgagat ttaaacccat attctgggta tactctatca
     7321 tattggtaca ttatactatc cctagtaact ttagcaagta tacttgctgc tgctattgat
     7381 atagattttg aatctccttt cacaattgga ttttgagata tgtcaatacc tggtattgtt
     7441 gctgcatcaa ctagtaaata atctggtgct ttttttaaac aatttatagc ctttttcatt
     7501 gccatatatg tagcatttaa tatattaaat tcatctattt cttcattgtt tactatacct
     7561 attccataat ctagtgcttc ttcttttatt acttcaaaaa gttcatctct ttttgcctca
     7621 ctcagtttct tagagtcatt tacaccttct attttggtat cttttttaaa tactacaaca
     7681 gaagctacaa caggtccagc aagtggtcct cttcccgcct catcaattcc tccaatatat
     7741 aaataacctt tatcatagcc ttcgttctca aatatattta tagtttccaa tctttcttct
     7801 tcttttctaa tattatccag cttttttgcc agttttatag ctagaccttg tacagatttt
     7861 ctttcatcaa ctcttaacag ctctatatat tccatatatt tttctacttc taaagtctct
     7921 attatttctt taatctccct cacactttta tcctgcatac cattccctca tctttcgttt
     7981 ttaaaaattt aatttttaaa tatattaaaa ttttttaatt agagtttaat tgcatccact
     8041 atagccacat tttaaacata tactacatcc acctgtatgt gcaattcctt cacttccaca
     8101 ctcaggacac acaaattttt catcttcttc aaaattttta tttattattt ctttggcttt
     8161 ttcatcttta ttatcttttt cttttttatt atttttataa tattcttttt tactattttc
     8221 ttttttatct tctttattat tttcttcctt tttttcattt attaaaattc cactcctttt
     8281 acatccatct ctaaatattg ttactccttt aagaccagcc tcccatgcct taatatatat
     8341 attgaaaaca tcttcaactg tagtttcaaa aggtatattt attgtagatg atatagaagc
     8401 atcaatatac ttttgccata ctttttgcat gtttattctt tcttcatatc ctatattcat
     8461 agcagtaaca aaaaaatctg gtaaacattc ttcatctgta atacctttct tgtccatata
     8521 ctctttaaca attggagtat atactttata atacacatcc tcatcatgca agctttcagt
     8581 ttttcttata tatgaaagat taaatatagg ttctattcca ccactgatac ctatcattgt
     8641 agatatggaa cctgtaggag gtatggttaa aagttgtgaa tttctaagcc catacttttt
     8701 aaccatttcc ttagtgtctt catttagatt ttctataaaa aacttagatt tagaaatatt
     8761 ttcatattta tactcactaa atgttccata ttctttagca attaatgctg attgcttcac
     8821 agcttcattt aacatagtct taccaattaa atcacatagt tctaaagatt catcagaacc
     8881 atatcttatt cccattttta taagcatatc agctataccc attacaccta aacctatctg
     8941 tctatactta tgtacactat ctctttgttc ttgtaatgga tgcaagttca atccctcatc
     9001 taagacatca tttaaagcta ctacagcttc tctaacgcaa gattttagtt tattaatatc
     9061 aaaagtagca tgttcaccaa atggttgtat tataaactct gacaagttta ttgagccaag
     9121 tagacaactt cctccacttg gaagtggttc ttctgcacaa ggatttactc ctgaaaagca
     9181 aaagttctta tcttcactca taaaattata tgtttttatt ttatcccaaa acaatatacc
     9241 tggttctgca taatcccagt tatttttcac aaatttatta aataaatcat aagcatttac
     9301 tatcttaact attttttctc cagtagtttc aacttcaaaa gtacaattat ataacttttt
     9361 ttctcttaca gcttgcataa attcatctgt tactcttaca gaaatatttg ctttagtaac
     9421 cttctccaaa tctgttttta tatctataaa gtcttctatc tctggatgat ttacatccat
     9481 agaaatcata agagctcctc ttctccctct ttgaccaatt aagccagttg tcatactgta
     9541 aagttccata aaagagactg ctccagtcgt atgttttgca gcattgttaa ctttcgctcc
     9601 ttttggtctt agctttgaaa tatcaattcc aactcctcca ccataactaa aagttctagc
     9661 aagttttttt gcagtatcaa atatatcttc tatgctatct tttggtggtt ctattacata
     9721 acaattagaa tatgttactt tttttccctc tttctgtaat cctctatttg ctaaaattct
     9781 tccagcaaat agaaatcttt tttgtcttat tagttttgat attcttgaac tattatttga
     9841 aattctcttt atccattcat caaaactctc accattatgt ctatacttgt tcttccatat
     9901 tgatttttgt aattcatcaa actcccaagc tttagtcctt atttcagagc gtctttctct
     9961 atataatatg tagtgttttg ctatatcttt tctatttgat tccataagta gtacttcaac
    10021 tatatcttgt atttcttcta cggataagca tttttccata gaattatagt cattagctat
    10081 ttttcttgat atatcatatg ccaactctct atctactcca ccttcactat ttatactagc
    10141 tttatagaca gcatcttcaa tcttattttt actaaaaagt tcttttgtac catctctttt
    10201 tattatgtat ttcattttta aacctccaat aaattagtat taaattaata cacacattaa
    10261 ctataactct aaacaaactt tttatatatt tgtatatttt catattaatt atctaacata
    10321 atatatatta tactatttta tattcaatat aacactcctt atagttttct taacttgtaa
    10381 aatatcttcc taaaaaactc aaaaataata taaaaaagta tataaaagtc aaattatact
    10441 cttatatact
//
LOCUS       DBGJFF010000133        10433 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.135, whole genome shotgun sequence.
ACCESSION   DBGJFF010000133 DBGJFF010000000
VERSION     DBGJFF010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10433
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.135"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            187..1509
                     /locus_tag="ACOIAF_003193"
     CDS             187..1509
                     /locus_tag="ACOIAF_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HGZ2670190.1"
                     /translation="MFDIDGRPPILKCLPLSMQHILAMIVGTVTVPIVVGGAVGVSPT
                     QITLLVQYALIIAGISTLIQLYPIGNIGSRLPIIFGVGFTYVPTLVAVGGNYGLASIL
                     GAQLIGGVATVLIGIFIKKIRKFFPNIVAGTVVFTIGLSLYPIAINYMAGGVNSPTYA
                     SMQNWAVAAITLVVVIGLSQFAKGYAKLAAIFLGIIVGYCVSLVLGIINFDPIREATW
                     FAIPKPLEFGMEFDMPVIISVVIVSIINAVQAIGDLSATTMGGMDRNITDKELSSGVI
                     GSGICTMIGALFGGLPPSSYSQNVGMVAMNKVISRFVLGIAGIFMLLSGFVPKFGALM
                     TTIPYSVLGGATISIFSMITMTGIKLIITEELSSRNITIVGLAIALGMGITSVPAAQE
                     AFPSFVKLVFGESPIVIATLVAFVLNLIIPNKSLEDEAKERAVMEETN"
     gene            1806..3167
                     /locus_tag="ACOIAF_003194"
     CDS             1806..3167
                     /locus_tag="ACOIAF_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HGZ2670191.1"
                     /translation="MKTRNTSKYDVDGIPPLKESIPLALQHLLAMIVGNMVPAILIAN
                     VVGLNQGQATMLIQGSMLAAGLATFLQLYPIPLFKGFKLGSRLPVMMGMSYVFLGACL
                     SVAAKDGLAALFGAQIAAGVIVFFVGFGVKKIRHIFTPIVSGTIIACMGLGLFATAIK
                     NLAGGEGTQTFGSPINFTVGVIVAFVIIMINKYGKGLVKNSSILIGILVGYAISLVLG
                     LVDFSAVQGAAIVSLPTPAAFGLEFRPELIVMFTIIYIIGIADMMGACTIATVGAMDR
                     EVTDEELASVVLGNSITSIISSLFAALPTGVFSQNTVIVSMNKVTSRFVIALGALVLL
                     LAGISPALGAIMTTIPSCVVGGATLVVFSSIAMSGFSIMSMDGFTEENNLIAGVSIAT
                     SMGLTTAPQVLDQFPETIGTVLGGSSIVSGAIIAILLQTLFKLKSRKSAENVTSNLEE
                     NIG"
     gene            3240..4634
                     /locus_tag="ACOIAF_003195"
     CDS             3240..4634
                     /locus_tag="ACOIAF_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HGZ2670192.1"
                     /translation="MIDILIKNAIIVTVNKDRDVIFDGAIAIQGDRILDIGNTVDIEP
                     KYLDAKKVIDANGKVIFPGFINTHNHLFQVLLKGLGDDMALHEWLNTMMFPSAKFLTE
                     QDTYDAAMLGCMEGLKSGITTMVDYMHTHNRPGLTDGIVKAYKDLGIRGVVGRGCIDL
                     GIHKELIEDVETVEKDLRRVFEKYHNSENGRIKICVAPSSMWAISEEMGKMLWNIVKE
                     YDSYFTVHISETEFARTATKDIHGEIDIKLLEKWGIVGPEVVAVHCVCITDEDMEMLK
                     KYDIKVSHNVASNMYLASGVAPVPEMLKKGINVSLGLDGAASNNAQDMVELMKLTALQ
                     HKVNNCDPLAISAEKVLEMATIEGARTLRMEDEIGSLEIGKKADLVIFNSMLSPKSIP
                     LHNPVSTLVYSASMHNVESVLVDGNVILENGKVTTIESEEQVLLNAQHAAEELCKRAN
                     VTNRMEGHKWNSLY"
     gene            4696..5166
                     /locus_tag="ACOIAF_003196"
     CDS             4696..5166
                     /locus_tag="ACOIAF_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HGZ2670193.1"
                     /translation="MEKIKLNINDYMYEIEVEKNETLLHVLREKLNLTGAKQGCNSGS
                     CGACKVLIDGVDMKSCKLLAKNCEGKVIYTIESFANGENLHPIQEAFIEVGAVQCGYC
                     TPGMIITTQALLNRNASPNEDEIREAFKENLCRCTGYVKIIEAVKLSAARLGGR"
     gene            5169..7385
                     /locus_tag="ACOIAF_003197"
     CDS             5169..7385
                     /locus_tag="ACOIAF_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HGZ2670194.1"
                     /translation="MEKVIGKSYPLKDAALKVTGQMKYVADMKPQNVLYAKMLLSPVA
                     HAKIKSIDTSEAEKVVGVRAIATYLNTSQRKYNGSLRFYEHKIPENEVIFSDTVRFVG
                     DRVAAVAAETPEIAEKAVKLIKVEYEELPAVFTIQDAIKEDAICIHGDSNIVSQNNIN
                     GGDVEKGFKECDYIFEDEYSTPAIHHSAIETHSVIAHYDYNDKLTVHTPCQNTFGFRI
                     ILSQIFGMSLNKVRVIRPAIGGSFGGKLELTIEPVAAALSKMTRRPVKMVLTRKESMI
                     STRTRHGSFVKLKTGVNKDGEVIAQDIKIYTNTGAYASSALNVIGALSHKVFKVYKIP
                     NIKFTGMPVYTNTPIAGAMRGYGSPQIFMAQQAQFAKIAKEIGMDLVDFQNKNAVEPD
                     DVDIIFHGSLGNPRVLDCIEQGKKMFKWDEKKKLPKEEGKYLRGIGMAIGAHGNGVFG
                     AHRDVVTLTLKLNEDGTLTLLTGAHDMGNGVVTMQTMMVAEVLGITPDKVDTFETDTD
                     ACTFNLGDYASRGVFVEGGGAKKTAEKLKNLILEEAEKLLETSKEDLYLDNGYVISKL
                     DENVKASLSDVAVYSQSKSLRELTVVEDYSSPAGRTSYGVHFAEVLVDKETKDIKVVD
                     FVAVHDVGRVINPLNLEGQLEGGIHMGLGYALSEELAFDEQGRPKATDFKTYKLFRTV
                     DMPKCQVAFVDGYEKAGPFGGKSIGECAVVPVAPAVANAVYNATDVEIHSLPIKLK"
     gene            7468..8139
                     /locus_tag="ACOIAF_003198"
     CDS             7468..8139
                     /locus_tag="ACOIAF_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HGZ2670195.1"
                     /translation="MAKILGICGSPRKGATEYAVLEALKEAETIPGIETEYWSVRGKK
                     ISPCVHCDACIRKETMCIIKDDIQELEQKILEADGIIVGSPVYDMNITAQLTAVFNRL
                     RPMYLVHPGKLQNKVGAAITTGGTRYGGQELTKLPIINFFLMHEMLVSGGLGGCYIGG
                     TIWSKDGKAKGAQEDETGMDTVKRLGKGAAEAVMVSKFGLEKWNVVKEELDVKKDEKS
                     PLRDH"
     gene            8502..9716
                     /gene="dpaL"
                     /locus_tag="ACOIAF_003199"
     CDS             8502..9716
                     /gene="dpaL"
                     /locus_tag="ACOIAF_003199"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HGZ2670196.1"
                     /translation="MLKEIKWKVNNLPKGDKENCIKFLNEEEITKVRNFHKSFPQYKE
                     TPLANLEGLAKKLGVAGVYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLDTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAQEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNHAAAFVSAPDWVSAKGMRTLGNP
                     LNGDEKVISGESGAVTTGLLVAAMEREDLADLRKDLKLDENSRILLISTEGDTDPDKY
                     RSIVWDGEYPSI"
     gene            9843..>10433
                     /locus_tag="ACOIAF_003200"
     CDS             9843..>10433
                     /locus_tag="ACOIAF_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HGZ2670197.1"
                     /translation="MLNEQRKQEVTEICQKLIQNPSSSGNEEGVVKAIEESFEKLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEGKIVDGKIYGRG
                     TSDMKGQYTAMMSAVAYFAKDTNRDFAGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKG"
BASE COUNT         3902 a         1221 c         2146 g         3164 t
ORIGIN      
        1 actaacaaat ataataaaaa ttatatatgt aaaacaatta tgttaaaaaa ttggcattat
       61 aattgcttca tattaagttg agatgacaaa ataaaaaaag aaaggatgtt gtgtgagata
      121 ataataactg tcattaaaaa atagttggga gtgataacct tgagtaaata tcaaaagaat
      181 gtttctatgt ttgatattga tggaaggcca ccaattttaa agtgtttgcc actatcaatg
      241 caacatatac tagcaatgat agttggaact gttacagtac ctatagtcgt aggaggagct
      301 gtgggtgtta gccctacaca aataacactt ttagtgcaat atgcattaat aatagctggt
      361 atatcaacac ttatacaact atatccaata ggaaatattg gttcaagatt gcctataatt
      421 tttggagttg gttttactta tgtaccaaca cttgtagcag tcggaggaaa ttatggatta
      481 gcaagcattt tgggtgcaca attaataggg ggagtggcta cagtattaat agggatattc
      541 ataaaaaaga taagaaaatt ttttccgaat atagttgcag gaacagtggt ttttacaatt
      601 ggattatctt tgtatcctat agcaataaat tatatggcag gtggtgtaaa tagtcctact
      661 tatgcctcta tgcaaaactg ggcagtagcg gctataacat tagtggtagt tataggtctt
      721 agtcaatttg caaaaggata tgcaaaattg gcagcaatat ttttgggaat tatagttgga
      781 tattgtgtat ctttagttct gggtataata aattttgacc ctattagaga ggcaacttgg
      841 tttgcgatac ctaaaccgct tgaatttggg atggaatttg atatgcctgt aataatctca
      901 gtagtaattg taagtataat caatgctgtt caagcaattg gagatttatc agctactaca
      961 atgggaggca tggataggaa tatcacagat aaggaattat caagtggagt aataggaagt
     1021 ggaatttgta ctatgatagg agctttgttt ggaggtttac caccatcatc atatagccaa
     1081 aacgtgggga tggtagctat gaacaaagta ataagtagat ttgtgcttgg aatagctggt
     1141 atatttatgt tattgtcggg gtttgtgcct aaatttggtg cattaatgac tactatacca
     1201 tattctgttt taggaggagc tacaatatct atatttagta tgataacaat gacaggtata
     1261 aagttaatta ttacagaaga gttgtcatca agaaatatta ccattgttgg tttggcaatt
     1321 gccttaggca tgggaataac atcagtacca gcagcccaag aggcatttcc ttcatttgta
     1381 aagttagtat ttggagaatc tccaatagta attgcaacat tggttgcatt cgttttaaat
     1441 ctaataatac caaataaaag tctagaggat gaggctaaag aaagagctgt tatggaagag
     1501 actaattaag atataataat ttaaaagaat tgtttaaatt atttagtatg agattgttag
     1561 attatttttg atagctattc attttaataa aaaatttaac aaataaatat ataccaggca
     1621 attaatgttc atttaagttg aaaatcgggg agggaatatt tagaaatttg ttaatttttt
     1681 ataaaaatag ctcagtttag ctgtgtttat cggttgagca ataaattcat ggtcataaaa
     1741 atatttatag aaaataatta taaaaatatt tagtcaagat atttatgtat gggaggattt
     1801 attttatgaa aacacgtaat acttcaaaat atgatgtcga tgggatacca ccactaaagg
     1861 aatcaatacc tttggcctta caacatctgt tagcaatgat agtaggaaat atggtacctg
     1921 cgatattaat agcaaacgtg gtaggactta accaggggca agcaacaatg ttgattcagg
     1981 gttctatgtt agcagcaggt ttagcaacgt ttttacagtt atatccaata cctttattta
     2041 aaggatttaa gttaggttca agattacctg taatgatggg aatgagttat gtatttttag
     2101 gagcttgtct ttcagtagca gctaaagatg gtttagcagc tctatttgga gcacagattg
     2161 ctgctggggt aatcgtattc tttgtaggat ttggagttaa gaaaattaga catatattca
     2221 ctccaatagt ttctggaact ataatagcat gtatgggact tggactattc gcaacagcta
     2281 taaagaattt agctggagga gaaggcacac aaacatttgg aagcccaata aacttcacag
     2341 taggagttat agttgctttt gtaataataa tgataaataa atacggtaaa ggtttagtaa
     2401 aaaattcttc aatattaata ggaatattag taggttatgc tatatcttta gttttaggat
     2461 tggttgattt ctcagccgtt caaggagcag ctatagtatc tttaccaaca ccagcagctt
     2521 ttggattaga atttagacca gagttaatag taatgtttac tattatttac ataataggta
     2581 tagctgatat gatgggtgcc tgtacaatag ctacagtagg agcaatggat agagaggtaa
     2641 ctgacgaaga attagcatca gttgttttag gaaattctat aacttctata atttcatcac
     2701 tatttgcagc tcttccaact ggagtattta gccaaaatac agtaattgta tctatgaaca
     2761 aagttacaag tagatttgta atagcactag gggcacttgt attattacta gcagggatat
     2821 ctccagcatt gggtgcaata atgacaacta taccatcttg tgttgttggt ggagcaactt
     2881 tagtagtttt ctcatcaata gcaatgtctg gtttctctat tatgagtatg gatggattca
     2941 ctgaagaaaa caatcttata gcaggtgtat ctatagcaac aagtatgggt cttacaactg
     3001 ctccacaagt tttagaccaa ttcccagaaa caattggaac tgtccttgga gggtcaagta
     3061 tagtttctgg agcaataata gctatattac ttcaaacatt atttaaatta aagtcacgta
     3121 aaagtgctga aaacgttact tcaaatcttg aggaaaatat tggataaatg tattagatat
     3181 aatatgagaa aaccatatgg taaaatgttg taatgaaagt attcaaaggg ggatttaaga
     3241 tgattgatat actaataaaa aatgcaataa ttgttacagt taataaagac agagatgtta
     3301 tatttgatgg agcaatagct attcaaggtg acagaatctt ggacatagga aatacagttg
     3361 atatcgagcc taagtatctt gatgctaaaa aagttataga tgctaatgga aaagtaatat
     3421 tccctggctt tataaatact cataatcacc tgttccaagt gttattaaaa ggtttaggtg
     3481 atgatatggc acttcatgag tggttaaata caatgatgtt tccatcagca aaattcttaa
     3541 cagaacaaga tacttatgat gctgcaatgc ttggatgtat ggaaggttta aaaagtggta
     3601 taactacaat ggttgattat atgcatactc acaatagacc aggacttaca gatggaatag
     3661 taaaggcata taaagattta ggcataagag gggttgtagg tagaggttgt atagatttag
     3721 gtattcacaa agagcttata gaagatgtgg aaacggttga aaaagatttg agaagggtat
     3781 tcgaaaaata tcataattca gaaaatggaa gaataaaaat ctgtgtagct ccttcttcaa
     3841 tgtgggctat atctgaagaa atgggtaaga tgctttggaa tatagttaaa gaatatgatt
     3901 catattttac agttcatata tctgaaacag aatttgcaag aacagcaaca aaggatatac
     3961 atggagaaat agatataaaa ttacttgaaa aatggggtat agtaggtcca gaagttgtag
     4021 cagtacattg tgtatgtata actgatgaag acatggaaat gcttaaaaag tatgacataa
     4081 aagtttctca taatgtagca agtaatatgt atcttgcttc tggtgttgca cctgtgccag
     4141 aaatgttaaa aaaaggtata aatgtgagtt taggccttga cggtgcagca agtaataacg
     4201 cacaagatat ggtagagctt atgaaattaa cagctttaca acacaaagta aataattgtg
     4261 accctctggc aatttctgca gagaaagttc ttgaaatggc tacaattgag ggagcaagaa
     4321 ctcttagaat ggaagatgaa ataggttctt tggagattgg gaaaaaagct gacctagtaa
     4381 tatttaactc aatgctttct ccaaaatcaa tacctcttca caatcctgta tcaactttgg
     4441 tatactcagc tagtatgcac aatgtagaga gtgttttagt tgatggaaat gtaatacttg
     4501 aaaatggaaa agtaactaca attgaaagtg aagaacaagt gttgcttaat gcacaacatg
     4561 cagcagaaga attatgtaag agagctaatg taactaatag aatggaagga cataagtgga
     4621 atagcttata ctaatattaa aataagattg taataatatt ctattacaac attagtttat
     4681 ctgtaggagg agaaaatgga aaaaataaaa ttaaatatta atgattacat gtatgaaata
     4741 gaagttgaaa agaatgaaac tcttttacat gtacttcgtg aaaaattaaa tctaactgga
     4801 gcgaaacaag gttgtaatag tggcagttgt ggagcatgta aagttttgat agatggagtt
     4861 gacatgaaat cttgtaaact tcttgcaaaa aactgcgaag gtaaagttat atacactata
     4921 gaaagttttg ctaatggaga aaatcttcat ccaatacaag aagcttttat agaagttgga
     4981 gctgtacaat gtggatattg tactcctggt atgattataa caacacaagc tcttttaaat
     5041 agaaatgcat ctcctaatga agatgaaatt agagaagctt ttaaagaaaa tctctgtaga
     5101 tgcacaggat atgtaaagat tatagaagct gtaaaattat ctgcagctag attagggggt
     5161 agataattat ggaaaaggta ataggaaaat cataccctct aaaagatgca gctttaaaag
     5221 ttactggtca aatgaaatac gtggctgata tgaaaccaca aaatgtactt tacgctaaga
     5281 tgcttttaag tcctgtagca catgcaaaaa taaaaagtat agatacaagt gaagctgaga
     5341 aagtagtagg tgttagagct atagctactt atctcaatac atcacaaaga aaatacaatg
     5401 gttcattaag attttatgag cacaaaatac ctgaaaatga agttatattt tcagatacag
     5461 ttagatttgt tggtgaccgt gttgcagcag tagctgcaga gactccagaa atagcagaga
     5521 aagctgtaaa acttataaaa gttgaatatg aagaattgcc agctgtattt actatacaag
     5581 atgcaataaa agaagatgct atctgcatac atggagattc aaatatagtt tctcaaaaca
     5641 acataaatgg gggagatgta gaaaaaggct tcaaggaatg tgactatata tttgaagatg
     5701 aatactctac tccagcaata catcatagtg caatagagac tcatagtgta attgcacact
     5761 atgattataa tgataaatta actgtacata caccttgcca aaatacattt ggatttagaa
     5821 taatactatc tcaaattttt gggatgtcat taaataaagt aagagttata agacctgcta
     5881 ttggaggctc atttggtgga aaattagaac ttacaattga accagtagca gccgcattat
     5941 caaaaatgac aagaagacca gttaaaatgg ttcttacaag aaaggaatct atgatatcaa
     6001 caagaactcg tcatgggtct tttgtaaaat taaaaactgg tgtaaataaa gatggtgaag
     6061 taatagcaca agatataaaa atatatacaa atacaggagc atatgcatca agtgctttaa
     6121 atgttatagg agcacttagt cataaagtat ttaaagttta taaaatacca aatataaagt
     6181 ttactggtat gcctgtatat acaaatacac caatagcagg agctatgaga ggctatgggt
     6241 cacctcaaat atttatggct caacaggctc aatttgcgaa aatagcaaag gaaataggta
     6301 tggatttagt cgattttcaa aataaaaatg cagtagagcc agatgatgtc gatataatct
     6361 tccacggttc tcttggaaat cctagagtat tggattgtat agagcaaggt aagaagatgt
     6421 ttaagtggga tgaaaagaaa aaacttccaa aagaagaagg aaaatattta agaggtatag
     6481 gtatggcaat aggtgcacat ggtaatggag tttttggtgc acatagagat gttgttacat
     6541 tgactcttaa attaaatgaa gatggtacgt tgacactact aacaggagct catgatatgg
     6601 gcaatggtgt tgtaactatg caaacaatga tggtagctga agttttagga ataacacctg
     6661 ataaagttga tacatttgaa actgatacgg atgcatgtac attcaacctt ggtgactatg
     6721 caagtagagg ggtatttgta gaaggtggag gagctaaaaa gacagcagag aaattgaaaa
     6781 atcttatatt agaagaagca gaaaaattac ttgaaacatc aaaagaagat ttatatctag
     6841 acaatggata tgtaattagt aaacttgatg aaaatgtaaa agcatcactt tctgatgtcg
     6901 cagtatatag tcaaagtaaa tcattaagag agttaactgt tgttgaagat tattcttcac
     6961 cagctggacg tacttcttat ggtgtacatt ttgctgaagt tttagtagat aaagaaacta
     7021 aggatataaa agtagttgat tttgtagctg tacatgatgt tggtagagtt ataaatcctc
     7081 taaatttaga aggacaatta gaaggtggga ttcatatggg tctaggatac gcattgagtg
     7141 aagaattggc atttgatgaa caaggtagac ctaaagctac tgattttaaa acatataaat
     7201 tatttagaac tgtagatatg ccaaaatgtc aagttgcatt tgtagatggt tatgaaaaag
     7261 ctgggccttt tggtggtaag agtattggag aatgtgccgt tgtaccagtt gcgccagctg
     7321 ttgcaaatgc agtatataat gcaactgatg tagagataca ttcattacca ataaaattaa
     7381 aatagtagaa attaaagcag aaataacaat ttattgtatt tgtaaataga taataaaaat
     7441 agatttattt ggtgaggaga tattattatg gcaaagatat taggtatttg tggaagtcca
     7501 cgtaaaggag caacagaata tgcagtgtta gaagctttga aagaagcaga aactatacct
     7561 ggtatagaaa ctgagtattg gtcagttaga gggaaaaaaa taagtccttg tgtacattgt
     7621 gatgcatgta taagaaaaga aactatgtgt attataaaag atgatatcca agaattagag
     7681 caaaaaatat tggaggcaga tggaattata gtaggttctc cagtttatga tatgaatata
     7741 actgctcaac ttacagcagt atttaataga cttcgtccaa tgtatttagt tcatccagga
     7801 aaacttcaaa ataaggttgg tgcagctata actactggtg gaactagata tggtggccaa
     7861 gaattgacaa aacttcctat aataaatttc ttcctaatgc atgaaatgtt agtgtctgga
     7921 ggtttaggtg gatgctatat aggtggtaca atatggagta aagatggaaa agcaaaaggt
     7981 gcccaagagg atgagacagg tatggacaca gtaaaaagac ttggaaaagg tgcagcagag
     8041 gcagttatgg tttctaagtt tggtcttgaa aaatggaatg tcgtaaaaga agaattagat
     8101 gttaaaaaag atgaaaaatc acctcttaga gaccactaaa gatttattat attattgaat
     8161 atatacaaat agtgatgtaa tagattattg catcactatt ttatgtttta atttagattt
     8221 tataaatttt atattaaaaa atatagaaac aaagtgtttt gagatttatt tttaaatttt
     8281 aaaaatgaga attaaaaagt gagaagatag aaaaggtttt ttagttatta aattctacat
     8341 acattgtatg aactaaagat agaaaagaaa aaataataaa agcattgcaa tttctatact
     8401 aaagagtcat ggaaaaatat ctagtatata tttagtattg gcatgttaat tgcttatata
     8461 ttaaagtata aaaatttaac aataatttgg aggtatttta gatgttaaaa gaaattaaat
     8521 ggaaagtaaa taatttgcca aagggcgata aggaaaattg tatcaaattt ttaaatgaag
     8581 aagaaataac taaggttaga aattttcaca aaagctttcc tcaatataaa gaaactccat
     8641 tagcaaacct agaaggttta gcaaaaaaat taggtgttgc aggagtatat gtaaaagatg
     8701 aatcttatag atttggatta aatgcattta aagtattagg tggttcttat tctatgggta
     8761 gatacttagc tcaaagatta gatacagata tatctgagtt agggtatgac aaattgactt
     8821 cgaaagaaat aaaagaaaaa ttaggagaaa taactttctt tacagctaca gatggaaatc
     8881 acggtagagg agttgcttgg acagctaata aattaggtca aaaatctgtt gtattaatgc
     8941 caaaaggttc ttctgaattt agattaaata aaattaaagg agaaggtgca gatgctagta
     9001 taacagactt aaactatgat gatgcagtaa gattagctaa tgattatgca gaagctgatg
     9061 accatggagt aatggttcaa gatacagcat gggacggata tgaagaaata ccagcgtgga
     9121 taatgcaagg atatggaact atggctcaag aagctataga gcaattaaaa gaatatggag
     9181 tagatagacc aacacacgta tttgttcaag caggagttgg ttcacttgct ggtgcagttc
     9241 aagggtatgt tgcttcaata tatgatgaat gcccaataac agtagtagta gaggctgatg
     9301 aagctgattg ttactataaa tcagcagaag ctggagatgg aaaaccaaga tttgttggag
     9361 gagatatgcc aactataatg gcagggcttg cttgtggaga accaaatact ataggatttg
     9421 aagtattaaa aaatcatgca gcagcatttg tttcagcacc agactgggtt tctgctaaag
     9481 gtatgagaac attaggtaat cctttaaatg gtgatgaaaa ggtaatttct ggagaatctg
     9541 gagcagttac aactggtctt ttagtagcag ctatggaaag agaagattta gcagatttaa
     9601 gaaaagattt aaaattagat gaaaattcaa gaatactttt aataagcaca gaaggagata
     9661 ctgaccctga caaatataga agtatagttt gggatggaga atatccaagt atataaggaa
     9721 ttactaaaat gcaattatta gatattggtt tacatattta gaagatatat aagttctaaa
     9781 atttagaaaa tatataagta tataaaatac atacattata aaaaatctag ggggataatg
     9841 aaatgttaaa cgaacaaaga aaacaagaag taactgaaat atgccaaaaa ttaattcaaa
     9901 atcctagttc ttctggaaat gaagaaggtg ttgtaaaagc aatagaagag tcttttgaaa
     9961 aattaggttt tgatagttgg tcaagagata gatatggaaa tatagtagga tgtataaaag
    10021 gtaacaagcc aggtaagaaa atattatttg atggacatat agatacagtt ccagttccag
    10081 atgcaagtaa atggagtgtt cctccttttg aaggaaaaat agttgatgga aagatatatg
    10141 gtagaggaac ttctgacatg aaaggtcaat atactgcaat gatgtctgct gtagcgtatt
    10201 ttgctaaaga cactaataga gattttgctg gagaactata tgtagcaggt gttgttcatg
    10261 aagaaatatt tgaaggtgtt tcagctagag agataagtaa ggctgttcaa cctgactatg
    10321 ttgtaatagg tgagtcttca gagttaaact taaagattgg tcagagaggt agaggagaaa
    10381 tagtagtaga aacttttggt aaaccagctc actctgctaa tccagaaaaa ggt
//
LOCUS       DBGJFF010000134        10382 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.136, whole genome shotgun sequence.
ACCESSION   DBGJFF010000134 DBGJFF010000000
VERSION     DBGJFF010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10382
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.136"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            196..757
                     /locus_tag="ACOIAF_003201"
                     /pseudo
     CDS             196..757
                     /locus_tag="ACOIAF_003201"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
     gene            complement(914..1366)
                     /locus_tag="ACOIAF_003202"
     CDS             complement(914..1366)
                     /locus_tag="ACOIAF_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staygreen family protein"
                     /protein_id="HGZ2670198.1"
                     /translation="MVNLNANNLNVNMVYPISSTAPIRFRRYTVSYLENMENIYLTIA
                     TYFDTLGLGTMNIDKVYGQWAWFTDNIYDLNLFVFVGNYPYQIAKNRYDTFVDILPVA
                     ITSIVNGDRVFLISNPCLLNSKITVRFISSHPTLNRTVDYGNIRDFIF"
     gene            1814..3172
                     /locus_tag="ACOIAF_003203"
     CDS             1814..3172
                     /locus_tag="ACOIAF_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HGZ2670199.1"
                     /translation="MFIRKNVIKLAIPIMIEQTFVMLLGVFNTMMAGHIGEEAVSAVG
                     MVDSINNIFISVFAALSVGATVVVAQQIGKKDNEKVNETIKQALVSGVILSFTITILM
                     WVFRTGVINLFYGSAEELVKSNAKLYIEFTLFTYPFIATQQIANGILRGCGDVKKPMY
                     VTMFMNIVNVTLGYILIYGIDLNPLGINLQTPSYGITGAAMSIAIARIIGCIIIIRAL
                     FKGNEFISMGKIFPFKIDVETQKSIFNIGIPAGVEQLLFNAGKLIVQVFIVTMGTSAI
                     ASNAIGMSIASIINVIGNALALSATTLVGQYIGRGDIKGAKSTLLYLTKFATICLFIV
                     GAIFIPIAPWISSMYTQNASVIDISTQLIRSDCLAMVIWPISFVLSAGLKGAGDTRYT
                     MMTAVIGMWIFRIFTGYVLGVVLEFGVLGIWIGMYTDWLVRGTLYCLRLRGTKWLKHK
                     VA"
     gene            3445..3825
                     /locus_tag="ACOIAF_003204"
     CDS             3445..3825
                     /locus_tag="ACOIAF_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGZ2670200.1"
                     /translation="MINKIGKITLYVNNQEDAKLFWTEKLNFVVTYEKEMGPNMKWVE
                     VAPSKNEFTTFVLYDKNLMMRQNKEINVSHPSIILSTRDINGTYEQMANNEVEVGELM
                     EMPYGRMFSFKDQDGNDYLVREDK"
     gene            3950..4150
                     /locus_tag="ACOIAF_003205"
     CDS             3950..4150
                     /locus_tag="ACOIAF_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670201.1"
                     /translation="MFKKMAVLKDIATKIGRKKAYELLEMVEGNDAFVAEVKIKKNGI
                     ESKKEEIMLKDNQKIILEYIEG"
     gene            4346..5074
                     /locus_tag="ACOIAF_003206"
     CDS             4346..5074
                     /locus_tag="ACOIAF_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2670202.1"
                     /translation="MLEIKNLSFNVESNNEELGIINDVSLSFERGKLIVITGPNGGGK
                     STIAKLIMGIEKATSGQIILDGEDITNLSITERAKKGIGYAFQQPPRIKGMTVENLLT
                     LAHGKPLSTDVCCQYLTDVGLCSKDYLNREVDNSLSGGEMKRIEIATLFARDLKVSIF
                     DEPEAGIDLWSFGKLNESFKKIHEESNQTIIIISHQERILELADEIIVLQDGSVKSHG
                     TKEAILPEIMCQVNSSCELMKDMN"
     gene            5096..6013
                     /locus_tag="ACOIAF_003207"
     CDS             5096..6013
                     /locus_tag="ACOIAF_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HGZ2670203.1"
                     /translation="MNKIDLNLLEKVAELRGTPLGAYNIRKNGQGVERHSTENVTIIP
                     KTDKPGIDIIVKPNTKGENVHIPVIVTETGVQDMVYNDFEIGENADVVIIAGCGIHNC
                     GCDDSEHNGIHRFFLRKGARLKYVEKHYGEGDGEGKRILNPVTIVNMDEDSYCEMETI
                     QIKGVDSTKREMDANLGKGAKIVVFEKLLTHGNQVAESNMKVILEGENSSAQVVSRTV
                     GQDNSKQKFNPCVIGDSLCSAHVQCDSIIMGNSQISAIPEIAANHPDALLVHEAAIGK
                     IAGDQILKMMTLGLTEEEAEQQILNCFLK"
     gene            6155..7339
                     /locus_tag="ACOIAF_003208"
     CDS             6155..7339
                     /locus_tag="ACOIAF_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HGZ2670204.1"
                     /translation="MKNLLIRNGTIISPTDGIKFEKRDILVLHGKIYAIGLNLKEKIR
                     KEKLLTNKEFEIINADNMYISPGFIDVHTHCYKRKLPTGMDSDSIGIEKGSTVIFDAG
                     TAGPLNYYDFKKKYMDECKTEVYSFLNVTNEGLNILNESTSLDVINFDKVESVVEKNI
                     EKIKGIKVKASKFQSNESGIDIIKKSKQVAKKLNLPLVVYIGEYPSYIDEVMNILGKG
                     DVVTPVYGNSTNGILKESGTLRKSVINAKNRGVLFDVCHGLNQFDFKVFKKAIKQGLE
                     PDLISTDMHIKNMKDVNYSLLNVINKIMELGISLEKCIEKVTEYPTKIFDLHGNKGRI
                     KIGGEGDFTIFTMEECNDIIKDYNNNELVLNKKLRLQYTVKSWMKSSEVYRHGYENTI
                     IN"
     gene            7446..7634
                     /locus_tag="ACOIAF_003209"
     CDS             7446..7634
                     /locus_tag="ACOIAF_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HGZ2670205.1"
                     /translation="MKAFVDKDVCVACGACIGTCPDVFSMSDEGHSVAIEGEIPADLQ
                     DLCKEAEEGCPVSAITVK"
     gene            7784..8434
                     /locus_tag="ACOIAF_003210"
     CDS             7784..8434
                     /locus_tag="ACOIAF_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HGZ2670206.1"
                     /translation="MKKNYEIVLFDLDGTLTDPKEGITKSIQYSLNSFGIKEDLENLD
                     QFIGPPLHDTFKEYYKFEDKKAKEAVEKYREYFADKGIFENKIYENIKEILEMLYKNG
                     KILLVATSKPTVFAETILRYFDIDRYFKYIAGSNLDGTRVNKNEVIQYVLDLCNVKDK
                     DKVIMVGDRKYDIIGAKKIGIDSIGVLYGYGSFEELSESEPTYIVENVESIKDILL"
     gene            8537..9475
                     /locus_tag="ACOIAF_003211"
     CDS             8537..9475
                     /locus_tag="ACOIAF_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGZ2670207.1"
                     /translation="MKSILIMGGSDFIGSALAKRLIKCGYQIDILTNGKKEIDYNGFK
                     EHLICDRKVRKDMENIITGRKYDYIYDMTAYTKEDVSNLIDFISMDNLKKYIVLSAGA
                     VYKDSGRNIKEENEKGENENWGKYGLNKKEAEDFIINSPIPYIIIRPTYIYGENNNLY
                     REYYFFEKIEKNEKIPVPKGKQVSNQFIYIGDLVKVLESIMKNPHVREAYNVTNPQLI
                     SWDDLIYTCGEIIGKEPIIKYVDMEKVEFRERTYFPFRNIDFNLDINKLIEHGLYIPN
                     VLLKEGLTATYKWFSANKPKMHDRKMNKVESVMQIV"
     gene            9597..10352
                     /locus_tag="ACOIAF_003212"
     CDS             9597..10352
                     /locus_tag="ACOIAF_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HGZ2670208.1"
                     /translation="MKKYNISENGILIKSDTIESKSERIKVVQISDTQIGSFYSTKNL
                     KKVANKINTLNPDIIVFTGDLIDYSNKNPSVDEITTILSSMNARLGKFSVFGNHDYMY
                     KLPRYYRQIMKNSNFNLLVNENKKIKLKDDKYINILGVDEILNGNPNIKYLESQIDNK
                     SFNLLLAHEPDLVDMLSKDTMNLVLSGHSHGGQIRLPIKGALVTPPYGRKYTKGFYDI
                     NGNHLYVSSGLGSTKLPFRFFNIPEIIEFNIII"
BASE COUNT         4218 a          995 c         1838 g         3331 t
ORIGIN      
        1 ttttaaggat ataaagtaaa aaaatataca atatcaagaa aaaaagatat tgggtattta
       61 aagtaatact ttatttaatt ttaagtaaat cgggtatcta catatataga tactaatttc
      121 aaagaatagg agggtgttta tatggaaata ttcgcaatag gacattcaaa ttatccatat
      181 gataagctta ttaatatgat aaaaaaatat gacataaatt gtgtagttga cattagagaa
      241 actccatatt ctaaatataa tattcaatac aataaagagg catttaacga atctcttaga
      301 aattccggat ttttatatat acatgggaaa agaatttgga gctaaaagaa ctaataaaga
      361 tgtatatacg caagaaggtt atgcagattt tgagaaagta gcaaaagaag atattttttt
      421 aaacggaata gaaagattaa aaaagggttg tcaaatgggt tataggatag ttttattagg
      481 agctatgcaa gaacctataa gatgtcatag aagtatcttg gtgggaaaag tattaaataa
      541 agaaggtttt gatgtaaaat atataatgca tgaaggaaat ttggcatatc aggaagatat
      601 agaggaatca ttattggata agtattttag tgatagaaag caactgagta ttgacaattt
      661 attagggagt gcattaacga gagaagaaat gatacaagaa ggttataagc tagctaataa
      721 agaaatagga tatagaactg agaaactagg taaataaaaa tatacaagta gataaaatac
      781 agtacttatc ttaagtaaaa taatattatg aatttataat agctaggcat gatgtgccaa
      841 ttaaaaatat tctagttatt taataatatc attaaattta gggtattttt taatagcaca
      901 tcatgttgca gttttaaaaa ataaaatctc taatattgcc ataatcaact gttctattaa
      961 gagtaggatg actagatata aatctaactg taatttttga atttaacaaa caaggatttg
     1021 aaattaaaaa tactctatct ccattgacaa tagaagtaat agctactggc aaaatatcta
     1081 caaatgtatc atatctgttt ttagcaattt gataaggata atttcctaca aaaacaaata
     1141 gatttaaatc atatatatta tcagtaaacc aagcccattg accataaact ttgtcgatat
     1201 tcattgtgcc taaaccaagt gtatcaaagt atgttgctat agttaaataa atattttcca
     1261 tgttttctaa atatgaaaca gtatatcttc tgaaacgtat gggtgctgtg gaagaaatag
     1321 gataaaccat atttacattt agattatttg catttaaatt aaccaaagta tcacatccta
     1381 aaattagtct ataaaataca atatttaaat ggatgcgatt ttgttacatc taaagtgtaa
     1441 tattttatat attgaaaaag tggtaaaagt ttggatgatt aaataatatt ataaaaaata
     1501 tataaaatgt acttattgtt attgctacac gatagtgata caatattaac gaaatatagc
     1561 tacttttaat aataacggaa aatatttata ttaaattaat aaatatttta aatgtggtat
     1621 ccataaaaaa taataaacat aagataatta taaagatata ctgtgttcaa gtgatattaa
     1681 acaagattta tattatttga aagcagtata ttataatgcg aacaatacaa ttctaaactt
     1741 tgcattgtca atttacagtt cgataaacta attaaaaatt aaatttatat aaagagggaa
     1801 aagaggaata gggatgttta taaggaaaaa tgttataaag ttagcaattc ctataatgat
     1861 agagcagaca tttgtcatgc ttttaggagt atttaataca atgatggcag gtcatattgg
     1921 ggaagaagct gtatctgcag ttggaatggt tgattcaata aacaatatat ttatatctgt
     1981 atttgctgca ttatctgttg gagctactgt tgttgtagct caacaaatag gaaaaaaaga
     2041 taatgaaaag gtgaatgaaa ctattaagca ggctctagtg tctggagtga tattatcctt
     2101 tacaataaca atattgatgt gggtttttag aactggtgta attaatttat tttatggttc
     2161 agcagaagaa ttggtaaaat caaatgcaaa attatacata gagtttacat tatttacgta
     2221 tccatttatt gcgacacaac aaatagcaaa tggtatttta agagggtgtg gagatgtaaa
     2281 gaaacctatg tatgtgacta tgtttatgaa tatagtaaat gtaacattag gatatatact
     2341 tatatatggt attgatttaa atcctctagg aataaatttg caaacaccat cctatggcat
     2401 aacaggtgca gctatgtcta ttgctatagc tagaataatt ggatgtataa taattataag
     2461 agctttattt aaaggaaacg aatttatatc tatgggtaaa atatttccat ttaagattga
     2521 tgttgaaact caaaagtcta tatttaatat agggatacca gctggagttg aacaactact
     2581 ttttaatgca ggtaaattga ttgttcaagt atttatagtt actatgggaa catcagcaat
     2641 tgcatccaat gcaataggaa tgtcaattgc atccataata aatgtaattg ggaatgcttt
     2701 agctttgtct gctacaacct tggtgggaca gtatatagga cgtggagata ttaaaggagc
     2761 taagagtact ttattatatt taactaaatt tgcaacaatt tgtttattta tagtaggagc
     2821 tatatttata ccaattgcac cctggatttc aagtatgtat actcaaaatg ctagtgtaat
     2881 tgacatctca acacaattaa ttcgaagtga ttgtttagct atggtaatat ggcctatatc
     2941 ctttgtatta tcagcagggc taaagggagc cggtgatact agatatacaa tgatgactgc
     3001 agtaataggt atgtggattt ttagaatttt tacaggttac gtattggggg ttgtattaga
     3061 atttggagtg ttaggaattt ggataggtat gtatactgat tggcttgtta gagggacttt
     3121 atattgttta agattaagag gaacaaagtg gctaaaacac aaagttgcat agaatatatg
     3181 aaatcacttt tagatggata actctattta gaagtgattt tttatgtagt tattatcata
     3241 attataagta tgatatataa ggtgattaaa ttacataaat aaagcctata atagtatgga
     3301 tactataaat tatatattga gcagaattac ataatttggc gataaatatt agaaatatat
     3361 atctatagaa aataatatat atgggtatat tattaaatat aaaaaatatt aggtatttat
     3421 ttaataataa aggtgggtat tgttatgata aataaaatag gaaaaataac tctctatgta
     3481 aataatcaag aggatgcaaa gttgttttgg acagaaaaat tgaactttgt agtaacttat
     3541 gaaaaggaaa tgggtccaaa tatgaaatgg gtggaagtag caccaagtaa aaatgaattt
     3601 acaacatttg tgttgtatga taaaaatcta atgatgagac aaaacaaaga gataaatgta
     3661 tctcatccat caataatttt aagtacaaga gatattaatg gaacttatga acaaatggca
     3721 aataacgagg ttgaggttgg ggaattaatg gagatgccat atggaaggat gttttctttt
     3781 aaagaccaag atggaaatga ttatttggtc agagaggata aataatttta caaagtatta
     3841 aatttactca atatctgata taattagaga caataattat tataaactag tctaggatta
     3901 gaattatata attaaaaatt aagtgtaaat tttgagtaga ggtgataaaa tgtttaagaa
     3961 aatggcagta ctaaaagata tagcaactaa aataggtcgt aaaaaagctt atgaactatt
     4021 agaaatggtt gaaggtaatg atgcctttgt agctgaggtg aagataaaaa agaatggaat
     4081 agaatctaaa aaagaagaaa ttatgttaaa agataatcaa aaaataatat tagagtatat
     4141 agaaggctaa tttttaaata catataagtt gatatactaa atttctgaga tgagtttaca
     4201 taaaactcat cttttttact tttcaataac tttgttttat tatttaatat acattttatc
     4261 tgcttagagt tgctgatata taaaaattac tgatataata aaagaatgga tattaatgta
     4321 atattttata gaaaagaggg gtattatgct tgaaataaaa aatctttcct ttaatgttga
     4381 gtctaacaat gaggaactag gaataataaa tgatgttagc ctttcatttg agaggggaaa
     4441 acttattgtt atcacagggc caaatggtgg aggaaaatct acaattgcta agcttattat
     4501 gggaattgaa aaagctacga gtggtcagat aattttagat ggagaagata taacaaatct
     4561 tagtattaca gaaagagcaa aaaaaggaat aggatatgca tttcaacaac caccaagaat
     4621 aaaaggtatg acagttgaaa atttacttac acttgctcat ggaaaaccat tgtctactga
     4681 tgtatgttgc caatatctta cagatgtcgg attgtgttca aaagactatt taaatcgtga
     4741 agttgataac tctctttctg gaggggaaat gaaaagaatt gagatagcaa ctctttttgc
     4801 tagagattta aaggtttcta tatttgatga acctgaagca gggatagacc tttggagctt
     4861 tgggaaactc aatgaaagtt ttaagaaaat acatgaagag tctaaccaga ctataatcat
     4921 aatttctcac caagaaagaa tattagagtt agctgatgaa ataattgttc tacaagatgg
     4981 ttcagtaaaa agccatggaa caaaagaggc tatattacct gaaatcatgt gtcaagttaa
     5041 ttcaagttgt gaattaatga aagacatgaa ttaattagaa aggaaggcaa attaaatgaa
     5101 caaaatagat ttaaatcttt tagaaaaagt tgctgaactt cgtggaactc cattaggagc
     5161 atacaatata cgtaaaaatg ggcaaggagt agagagacat tcaactgaaa atgtaactat
     5221 aattcctaaa actgataaac ctggaataga tattatagta aagccaaata caaaaggaga
     5281 aaatgtacat ataccagtaa tagtaacaga gactggtgta caggatatgg tatataatga
     5341 ttttgaaatt ggagaaaatg cagatgttgt tataattgct gggtgtggta tacataactg
     5401 tggttgtgat gatagtgaac ataatggtat acatagattt ttcttacgta aaggagctcg
     5461 cctaaaatat gtagaaaaac attatggtga aggtgatgga gaaggaaaga gaattttaaa
     5521 tccagttact attgtcaata tggatgagga ttcatattgt gaaatggaaa caattcaaat
     5581 taagggtgta gattcaacta aaagagaaat ggatgcaaac ctaggtaaag gagctaaaat
     5641 agttgtattt gagaaattat taactcatgg taatcaagtt gcagaatcta atatgaaggt
     5701 tatcttagag ggggaaaact cttctgctca agtagtttct cgtacagttg ggcaagataa
     5761 ttctaagcaa aagtttaatc cttgtgttat tggggattcc ctttgttctg cacatgttca
     5821 atgtgattca ataattatgg gaaattctca aattagtgct atacctgaaa ttgctgcaaa
     5881 tcatccagat gctcttttag tacatgaagc agctattgga aagattgcag gagaccaaat
     5941 actaaagatg atgacattag gtcttactga agaagaagcc gaacaacaaa tacttaattg
     6001 cttcttaaag taaattaaag attttattta atttaatata attaatatag tataatatag
     6061 tgaataagtc taaaatattt ttatttttag gcttattttc tttatgtaag aaaagctatt
     6121 agagaaatta tattgtaatt aggatgtgga ataaatgaaa aatttactta taagaaatgg
     6181 gacaataata tctccaacag atggtatcaa atttgaaaaa agagatatct tggttttaca
     6241 tgggaaaata tatgctattg gattgaacct taaagagaaa ataagaaaag aaaaattact
     6301 tacaaataaa gagttcgaga taataaatgc cgataatatg tatatttccc caggatttat
     6361 agatgttcac acccattgtt ataagagaaa attacctaca ggtatggatt ctgattcaat
     6421 tggaattgaa aaaggttcta cagttatatt tgatgcaggt acagctggtc ctttaaacta
     6481 ctatgatttt aaaaaaaagt atatggatga gtgtaaaaca gaagtatatt cttttttaaa
     6541 tgttacaaat gagggactaa atatattgaa tgaatcaact tctttagatg ttataaattt
     6601 tgacaaggtt gagtcagttg tagaaaaaaa tatagaaaag ataaagggta taaaagtaaa
     6661 agcgagtaaa tttcaatcta atgaaagtgg tatagatata attaagaaat caaagcaagt
     6721 tgctaaaaaa ttaaatttac ccttagtggt atatatagga gagtatccat catatataga
     6781 tgaagtaatg aatattttgg gaaaaggaga tgtggttaca ccagtatatg gaaatagtac
     6841 aaatggaatc cttaaagaat ctggcacatt aagaaaatca gtaataaatg caaagaacag
     6901 aggagtattg tttgatgtat gtcatggatt gaaccaattt gattttaaag tttttaaaaa
     6961 agcaataaag caaggtttag agccagacct aataagtact gatatgcata taaaaaacat
     7021 gaaagacgta aactattctc ttttaaatgt tataaataaa attatggaac ttgggatatc
     7081 cttagaaaag tgtatagaaa aagtaactga atacccaaca aagattttcg atttacatgg
     7141 aaataaaggc agaataaaga ttggtggaga aggtgatttt acaatattca caatggagga
     7201 gtgtaatgat attataaaag actacaataa taatgaactt gttttaaata aaaaattaag
     7261 attgcaatat actgtaaaat catggatgaa aagcagtgaa gtttatagac atggatacga
     7321 aaatactata ataaactaaa caatagtgat aaaataataa atatatgttt aaattggtat
     7381 atattggata aatatctaag gaaacaacaa ttaaaaaata ataattttag gggaggtatt
     7441 ttagtatgaa agcatttgtt gataaggatg tatgtgtagc ttgtggagca tgtataggaa
     7501 catgtccaga cgtttttagt atgagcgatg aagggcattc agtagcaatt gaaggagaaa
     7561 taccagcaga cttacaagat ttatgtaaag aagccgaaga aggatgtcca gtttcagcta
     7621 taacagttaa atagagctgt ttaaatttat ttttaaaata aatatataag atttaattgg
     7681 gtacttaagc tatagtatta gaaatttcta ttactatagc tttttaaaat atataaactt
     7741 aaaatataag atattacttt ttacaaatat ggaggactga aatatgaaaa aaaattatga
     7801 aatagtatta ttcgatttag atggaacact tacagaccct aaagaaggaa taacaaagtc
     7861 tattcagtat tctttaaata gctttggcat aaaagaagat ttggaaaact tagaccaatt
     7921 tataggacca ccattacatg atacttttaa ggagtattat aaatttgaag ataaaaaagc
     7981 taaagaagct gttgaaaagt atagagaata ttttgctgat aaagggatat ttgaaaataa
     8041 aatttatgag aatataaaag agatattaga aatgctctat aaaaatggta aaatattatt
     8101 agtagcaact tctaagccaa cagtttttgc agaaactata cttagatatt ttgatataga
     8161 tagatatttt aaatatatag caggtagcaa tcttgatggt acaagagtga ataaaaatga
     8221 agttatacaa tatgtactag acctatgtaa tgtaaaagat aaagataaag ttattatggt
     8281 tggagataga aagtatgaca taattggagc caaaaaaata ggtatagatt ctattggagt
     8341 gttgtatggg tatggttctt ttgaagaact tagtgaaagt gaacctactt atattgtcga
     8401 aaatgtagag tctataaaag atattttgtt ataaatatct tttatagaca aaaatattac
     8461 aaaatggtgt cgcatattta aaaatatgat attatagtta aggaagtcat ttaaaaaaga
     8521 ggaggatgca cttaaaatga aatctatttt aattatgggt ggaagcgact tcataggttc
     8581 tgcacttgct aagcgcttga ttaaatgtgg gtatcaaatt gatatactta caaatggtaa
     8641 aaaggaaata gattataatg gttttaaaga acatctaatt tgtgatagaa aagttcgaaa
     8701 agatatggaa aacattatca caggaagaaa atatgattac atatatgata tgacagcata
     8761 tacaaaagaa gatgtaagta atctgattga ttttataagt atggataact taaaaaagta
     8821 tatagtattg tcagcaggag cagtatataa agatagtgga agaaatataa aagaagaaaa
     8881 tgagaaaggt gaaaatgaaa actggggaaa atatgggtta aataaaaaag aggctgaaga
     8941 ttttattata aatagcccta taccatatat aattattaga ccaacttata tatatggtga
     9001 aaataataat ttatatagag agtattattt ctttgaaaaa atagaaaaaa atgaaaagat
     9061 accagtacca aaaggaaaac aagttagtaa tcaatttata tatattgggg atttggtaaa
     9121 ggtattggaa agcataatga aaaatcctca tgtaagagag gcctataatg tgacaaatcc
     9181 acagttaatt tcttgggatg accttatata tacatgtgga gaaattattg gcaaagaacc
     9241 tataataaaa tatgtagata tggaaaaagt agagtttaga gaaagaacgt atttcccatt
     9301 tagaaatata gactttaatc ttgatataaa taagcttatt gaacatgggc tttatatacc
     9361 aaatgtgtta ctaaaagaag gtcttacagc aacatataaa tggttttcag caaataagcc
     9421 aaaaatgcat gacagaaaaa tgaacaaagt tgagtctgta atgcaaatag tttaaaaaaa
     9481 taaacaagta ggtagatatt ttgaactcaa aaattaagta ttttttaaaa ttttttatct
     9541 taatatttat tttgattttt atatactcag ttgcgataga gccgagtcta ttaattgtga
     9601 aaaaatataa tataagtgaa aatggaatat tgataaaaag tgatacaatt gagagtaaat
     9661 ctgaaaggat aaaagtagtt caaatttcag atactcaaat aggtagtttt tattcaacta
     9721 agaatctcaa aaaagtagct aataagatta acactttgaa tccagatata atagtattta
     9781 ctggtgattt aattgattac tccaataaaa atccaagtgt agatgaaatt acaactattt
     9841 tatcaagtat gaatgctagg ttgggaaaat tttcagtatt tggcaatcat gattatatgt
     9901 ataaacttcc taggtattat aggcagatta tgaaaaattc caacttcaat cttttagtaa
     9961 atgaaaataa aaaaataaaa ttaaaagatg ataaatatat taatatatta ggtgttgatg
    10021 aaatattaaa tggaaatcct aatataaaat atctggagag tcaaatagat aataaaagtt
    10081 ttaatttatt gttagcacat gaaccagatt tagtagatat gcttagtaaa gatacaatga
    10141 atttagttct atctgggcat agtcatggtg gtcaaataag attacctatt aagggagcat
    10201 tagttactcc tccatatgga aggaaatata ctaaagggtt ttatgatata aatggaaatc
    10261 atttatatgt gagttcgggt cttggaagta caaaattacc atttagattt tttaatatac
    10321 cagagataat agaatttaat attataatct aacataaaat atttagaaaa tataattttg
    10381 ct
//
LOCUS       DBGJFF010000135        10317 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.137, whole genome shotgun sequence.
ACCESSION   DBGJFF010000135 DBGJFF010000000
VERSION     DBGJFF010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10317
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.137"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            282..1706
                     /locus_tag="ACOIAF_003213"
     CDS             282..1706
                     /locus_tag="ACOIAF_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HGZ2670209.1"
                     /translation="MSSSNQSTIKTSIQRIGKFLSGMVMPNIGAFIAWGLITALFIPT
                     GWMPNEKLSTIGDPMIKYLLPLLIAYTGGKAIAGQRGGVIGAAAAMGVIVGADIPMFI
                     GAMIMGPFAGWVIKKFDKFVDGKIPTGFEMLVNNFSIGIIGMLLAILGFYAIGPAIVA
                     GTALIESGVQFIVSKSLLPLVSVFIEPGKVLFLNNAINHGILGPIGIAEAKEAGKSIM
                     FLLESNPGPGLGVLLAYWMFSRGSVKQSAPGAVIIHFFGGIHEIYFPYILMNPVLILA
                     TMAGGAAGILTFSILGAGLVAAPSPGSIFALMALAPKGGLLPVLAGVAVATVVSFLVA
                     APFVKRASANQSEEDSTSLEEAKAKMSDMKSASKNSEKNIEEKQLEVNEIKKIVFACD
                     AGMGSSAMGASRFKNRIKNLDLNIEITNSSVDNLPDDTQIVVTHNTLVERVAKNNSSV
                     EIVSINNFLNDPNLDALFKRLESK"
     gene            1732..3870
                     /locus_tag="ACOIAF_003214"
     CDS             1732..3870
                     /locus_tag="ACOIAF_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HGZ2670210.1"
                     /translation="MDNLKKKKITSRQKKIILMIVENSKKNIPITISEIAGTLELSSR
                     TVLRDMSGIEKWFDENDFNFVKKPGVGLILEENIENQNFIIELLEEEKIEKEYSKEER
                     NLIILSKLLVSNEPVKSYYFAKILKVSEGVLNNDFALASKWLERFDIELVRKPGLGVY
                     LKGQEKNFREAYVNLIYDSFNEKEILDMVRNISENIQTDKAIEILSENRLLNLMDRCI
                     IRKVEATLTKKLSDLDVNLADSAYIGLVVHISLALQRIKNGENITMDKEFLKELSITE
                     EFKLAKEIVKGMETDFNMDIPVDEVGYITMHIRGAKQRSSSNHKALNLDDIEIMEITN
                     KMIDLAEDEFKISLKNDERLFKDLANHLGPSINRLNMGLEIRNPLLDEIKSKYSYAYD
                     GVEKISRIIKDKLNINSIPESEIGYIAMHFASAIEKNLMMNTNINIVVACPTGIGTSR
                     FLSTKIENKFPNLNILETISAINIDEEYLKEKDVDLIVSTVELNTSLNYICVGPFMSL
                     DDEQIIKEKIKSIAQNKLINLNAKNDTKSKNKVYEQITESMNIGKDILQFLEEIRFEK
                     FESKDLSELIEDSSRIFAKSSEDIISIKESLKERLKISIPYIEESKILLLHCMSERID
                     IMKLSIIKLENRIVLDSNEEIDNVVFMLLPKNSPSYQRQIMSEISGSLIDNIIFTNKI
                     NKFSIEEMALEVKDIVFNFYTNRLKAFIDK"
     gene            3900..4322
                     /locus_tag="ACOIAF_003215"
     CDS             3900..4322
                     /locus_tag="ACOIAF_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGZ2670211.1"
                     /translation="MSKVLNENNIFLGLDSVSKEEAITLAGRKLVENGYVKEEYIPAM
                     LEREKVMTTYMGMGVAIPHGVNEAKKEILSSGIVILQFPNGIDFDGEKAYLLIGIAGV
                     GDEHLEILSNIAVVLDDDLTERLKNSNDKQAFMEAFAN"
     gene            4341..5492
                     /locus_tag="ACOIAF_003216"
     CDS             4341..5492
                     /locus_tag="ACOIAF_003216"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HGZ2670212.1"
                     /translation="MKKAIQFGAGNIGRGFIGGLLVKSGYHVVFADVNEYILNSINKD
                     KKYTIHIRDVECIDEVIDNISAVSSIKEEIIDEIVQAEIITTAVGPLVLTKIASTIAK
                     GIKARKEKGLTSNLNIIACENAIYASSSLKEEVLKYLNKEEVEYLEMYVGFPNCSVDR
                     IVPPGKNENPLDVTVENFYEWNVEKQGFKGEIPTIVGMNLADNLMAYIERKLFTLNTG
                     HAITAYIGYLKGYKTIEESIKDKFICDIVKSAMVESGEGLIKKYNFDSEVHYKYIDKI
                     LNRFKNPYLNDDVLRVGREPLRKLSDKDRLIKPLMTAKSYGLSVDNLILGIGAALHYN
                     NSEDTQSVELQELIKSIGVKKAVAKIANISNDEELLNNIEKSYIFMKNL"
     regulatory      5598..5699
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            5882..6454
                     /locus_tag="ACOIAF_003217"
     CDS             5882..6454
                     /locus_tag="ACOIAF_003217"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HGZ2670213.1"
                     /translation="MKALQEKILREGSVSGNDILKVDSFLNHQIDVAFLNEIGREFKE
                     RFKGEKVDKIFTIEASGIAIASIVSQYFDNAPVVFAKKSESKNLDKDVYETNVYSFTK
                     AREYSVKVSKKYINKGENILIVDDFLANGRAALGLKDLIEQAEANLVGVGIVIEKGFQ
                     AGGALLKANDVRLESLAVVESIDNGTVKFR"
     gene            6518..7168
                     /gene="fsa"
                     /locus_tag="ACOIAF_003218"
     CDS             6518..7168
                     /gene="fsa"
                     /locus_tag="ACOIAF_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HGZ2670214.1"
                     /translation="MRFFIDTANIEEIKEANDLGVICGVTTNPSLIAKEGRDFIEVVK
                     EISEIVDGPISAEVISLEHKGMIEEAEKLSKIHKNIVIKIPMTAEGLKAVKVLSSKGI
                     KTNVTLIFSAGQALLAARAGATYVSPFVGRLDDISQNGLDLIEEIVDIFSVNSIEAQI
                     IVASVRNPIHVLQAARMGADIATVPLKVINQMIKHPLTDKGIDSFMKDWEGASLKL"
     gene            7320..10244
                     /locus_tag="ACOIAF_003219"
     CDS             7320..10244
                     /locus_tag="ACOIAF_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HGZ2670215.1"
                     /translation="MDKILREIQREDKKNPYTDQELAEMLNVARSEVISVRKKNNILD
                     SRERRKKILIKDISKIVSENPQMSERKITEILIENGYNISRSAVSKLLKEENLNQSKI
                     GIKDKDPASSGKYVKVSKVVEEDGFEELIGIKGSLKEKVNLAKSAIMYPPNGLHTIIY
                     GETGVGKSELATCMYKYAIKNNIKEENSPFIVFNCADYAENPNLLIAQLFGVVKGAYT
                     GADANREGLVEQANNGILFLDEIHRLPAAGQEILFSLIDRGEFRRLGESSSNRKANVL
                     IISATTENPDSNLLQTFRRRIPMVINLPNMSERPKSERYEIIIKFFEREAKRVNKNFI
                     ITKEVMCALMNYKCTGNIGQLKSDIQVTCARAFSKSIFSSDKVIIDLDSLRDYIKSGY
                     YDCNCKNEKYSEFSVEDIYIDISELVSGKNRILSDNEISEIYNYAEKELKVLESKCFS
                     EEELKNAFIEKLDKKFNAIKNNKNLSDRRSRIDWGGQLKESTLEIMDKVIVFIKEQFK
                     HVNQGLYLALAIHIEHAIGRIKDGKTIINPSLDKIKASMPMEYELSRYIMGIVEDLTQ
                     VTFPEDELGYLAYYINKFCYNEESIKDKVKVVIVTHGKVGIEMSKVVNHILGIECTLG
                     IEIALTDSPSEGIEHVLEELQKIEARKGILVLIDMGSLVILGDEVEKRLGIRCKTVNR
                     VDTLLAMEAGKLATIEGKSLDGIIADLKKNKNYAMINTNKFSYRKNEYGKKNVIITLC
                     LSGVGTALNLKEHIEKQIKEYDTLIEVKPVAFLNNNLEEELNDIESEYNIIAICGTID
                     IDYKNVPFISYNEVLGKNGINKVLEHLNSKQPALNIENQLNNLIHEDLILYNFEGISK
                     EYIIDTLVSKLEEGGYVDSKYILSVYKREAMGSVVMASKVAVPHGLPENVIKPAIAIA
                     RLNKPIVWDNKFMVDLVVLLALKENNKKEIRSLFSKINDQHTLEVLLNTEDKNEIKEL
                     LS"
BASE COUNT         4230 a         1006 c         1939 g         3142 t
ORIGIN      
        1 tatatccaaa agtacattta ataaatttat ttaactgaat tattattttc atttctatta
       61 aatttaaata ttttataaaa atagtattat ttacaaaatc atatactcca aaatactaca
      121 taaataaaat taaacaaaaa ctaaaatgac aaatgtcatt atctaaagtg acatagaaac
      181 ggtttcttaa agttgttact ataaaatata aactctataa taaaagtata acaaaaggga
      241 atcatatcga tatagaatat aattcaagag gaggtaaaat tatgagtagt tctaatcaat
      301 cgacaataaa gacatctata cagcgaatag ggaaattttt aagtggtatg gtaatgccaa
      361 atataggagc ttttatagca tggggtctta taacagcatt atttatacca acaggatgga
      421 tgcctaatga aaaattgagt actataggtg acccaatgat aaaatacctt ttaccactat
      481 tgatagctta tactggtggt aaagcaatag caggacaaag aggtggtgta ataggtgcag
      541 cagcagctat gggagtaatt gttggtgcag atataccaat gtttattgga gcaatgataa
      601 tgggaccatt tgcaggatgg gtaattaaga agtttgataa atttgtagat ggaaagatac
      661 caacaggatt tgaaatgtta gtaaataact tttcaatagg tataataggt atgcttcttg
      721 caatactagg attttatgca attggaccag caatagtagc agggacagct cttatagaat
      781 ctggagttca atttatagtt agcaaaagtt tattgcctct agtttcagta tttatagaac
      841 ctggaaaagt attattctta aacaatgcaa taaatcatgg tatattaggg cctataggta
      901 tagctgaggc aaaagaagca ggtaaatcta taatgttctt actagaaagt aaccctggac
      961 caggtttagg agttctatta gcatattgga tgtttagtag aggaagtgta aaacaatcag
     1021 caccaggagc tgtaataata catttctttg gtggtatcca tgaaatttac ttcccatata
     1081 ttttaatgaa tccagtttta atattggcta caatggcagg tggagcagca ggaatattga
     1141 ctttttcaat attaggagct gggcttgtag cagcaccatc accaggaagt atatttgcac
     1201 ttatggcact agctccaaag ggaggattac ttccagtatt agcaggagtt gcagtggcaa
     1261 cagttgtatc attcttagtg gcagcaccat ttgttaagag agcttcagca aatcaaagtg
     1321 aagaagacag tacttcttta gaagaagcta aagcaaaaat gagtgatatg aaatctgcaa
     1381 gtaagaactc tgagaaaaat attgaggaaa aacagcttga agtaaatgag attaagaaga
     1441 ttgtatttgc atgtgatgca ggaatgggtt cgagtgcaat gggagcttcc agatttaaaa
     1501 atagaataaa aaacctagac ttaaatatag aaattacaaa ttcatctgtt gataatcttc
     1561 cagatgatac acaaatagta gtaactcaca atacattagt tgaaagagtt gctaagaata
     1621 attcaagtgt tgagatagtt tcaataaaca attttttaaa tgatccaaat ttagatgcat
     1681 tatttaaaag attagaatct aaataaaaat aaaaagtacc actaattagg agtggataat
     1741 ttgaaaaaaa agaagattac atctaggcaa aaaaagataa tactaatgat agtagaaaat
     1801 tcaaagaaaa acattccaat aactatttct gagattgcag ggactttaga gttaagttca
     1861 agaactgtac ttagagatat gtctggaatt gaaaaatggt ttgatgaaaa tgattttaac
     1921 tttgtaaaaa aaccaggagt gggattgatt ttagaggaaa atatagaaaa tcaaaacttt
     1981 ataattgagt tgttagaaga agaaaaaata gaaaaagaat acagcaagga agaaagaaat
     2041 ttaattatat tatcaaagct actagtttct aacgagccag taaaatccta ttattttgca
     2101 aaaatattaa aggtatctga aggggtactg aataatgatt ttgccttagc aagtaaatgg
     2161 ttggaaagat ttgatattga gcttgttaga aaaccagggc ttggtgtcta cttaaaaggt
     2221 caagaaaaaa actttagaga ggcatatgta aatcttatat acgattcatt caatgaaaaa
     2281 gaaatactag atatggtcag aaatataagt gaaaatatac aaacagataa agcaatagaa
     2341 attttaagtg aaaatagact tttaaattta atggacaggt gcataataag aaaagttgaa
     2401 gctacattaa caaagaagct ttcagattta gatgtaaatt tagctgatag tgcatatata
     2461 gggcttgtag ttcatatttc attagcctta cagaggataa aaaatggtga aaatataact
     2521 atggataaag agtttctaaa agaattatct ataacagaag agtttaaatt agctaaagaa
     2581 atcgtaaaag gtatggaaac agattttaac atggatatac cagtagatga ggttggatat
     2641 ataaccatgc atataagagg tgcaaagcaa aggtcatctt caaaccataa ggctttaaac
     2701 ttagatgata tagaaataat ggaaattaca aataagatga tagatttggc ggaagatgaa
     2761 tttaaaataa gtttaaaaaa tgacgagagg ttatttaaag acctagcaaa tcacttaggg
     2821 ccttctataa atagattgaa tatgggatta gaaataagaa atcctctttt agatgaaata
     2881 aaaagcaagt attcatatgc ctatgatgga gttgaaaaga tttctagaat tataaaagat
     2941 aaactaaata taaattcaat acctgaatca gaaataggat atattgcaat gcactttgca
     3001 tcagccatag aaaaaaatct tatgatgaat actaatataa atattgttgt ggcatgtccc
     3061 actggaatag gtacttcaag attcttatca actaagattg aaaataaatt tcctaattta
     3121 aatatacttg aaaccatatc agctataaac atagatgaag agtatttaaa ggaaaaagat
     3181 gttgatttaa tagtgtctac agttgagtta aatacaagtt taaattatat atgtgtaggt
     3241 ccatttatga gtttagatga tgaacagata atcaaggaaa aaataaaatc tatagctcaa
     3301 aataaattaa tcaatttaaa tgcgaaaaat gatactaaga gtaagaataa agtatatgaa
     3361 cagataacag aaagtatgaa tataggaaaa gatattcttc aatttttaga ggaaattaga
     3421 tttgaaaaat ttgaaagtaa agatttaagt gagcttattg aagactcatc aagaatattt
     3481 gcaaaatcta gtgaagatat aatctctata aaagaatcgt taaaagaaag attaaaaatc
     3541 tcaattccat atatagaaga atcaaaaatt ctattgttac attgtatgag tgaaagaata
     3601 gatattatga agttatcaat aataaaacta gagaatagaa tcgtgctaga ttcaaatgaa
     3661 gaaatcgaca atgtagtttt tatgttactt cctaaaaatt caccaagtta tcaaagacaa
     3721 ataatgagtg aaataagtgg ttcattaata gacaatataa tttttactaa taagataaat
     3781 aaattctcta tagaagagat ggcattagag gtaaaagata tagtttttaa tttttataca
     3841 aacagattaa aagcatttat agataaataa aattaatatt aaaaaggaag gttgatacta
     3901 tgagtaaagt attgaatgag aataatatat ttttagggtt agatagtgtt tcaaaggaag
     3961 aagctataac tttagcagga agaaaattag ttgaaaatgg atatgtaaaa gaagaatata
     4021 taccagcaat gttagaaaga gaaaaagtaa tgactacata tatgggaatg ggagttgcta
     4081 ttccacatgg tgtaaatgaa gcaaaaaaag aaatattatc ttctggaata gttatattac
     4141 aattccctaa tggtatagat tttgatggag aaaaagcata tttattaata ggaatagctg
     4201 gtgttggaga tgaacattta gaaatactat ctaatatagc agtagtttta gatgatgact
     4261 taacagaaag actaaaaaat tcaaatgaca aacaagcttt tatggaagct tttgccaact
     4321 aatcttagga gggatttaaa atgaaaaagg caattcagtt tggagcagga aatattggaa
     4381 ggggttttat tggaggcttg ttagttaagt cgggttatca tgtagtattt gctgatgtaa
     4441 atgagtatat attaaactca ataaacaaag ataaaaagta tactatacac ataagagatg
     4501 tagaatgtat agatgaagta attgataata taagtgcggt ttcatctata aaagaagaaa
     4561 taatagatga gattgtacag gctgaaataa taactacagc agttggacct ttagttttga
     4621 ctaaaatagc ctctacaatt gcaaaaggta taaaagctag aaaagaaaag ggattaacaa
     4681 gtaatctaaa tataatagca tgtgaaaatg caatatacgc aagttcttct ttaaaagaag
     4741 aggtacttaa gtatttaaat aaggaagaag tagaatattt agagatgtat gttggttttc
     4801 caaactgttc agttgataga atagttcctc ctggtaaaaa cgaaaatcct cttgatgtaa
     4861 cagttgaaaa tttttatgag tggaatgttg aaaaacaagg atttaaaggg gaaataccaa
     4921 ctatagttgg aatgaattta gctgataatc ttatggctta tatagaacgt aaattgttta
     4981 cactgaatac aggtcatgct ataacagctt atataggata tttaaaagga tataagacta
     5041 tagaagagag tataaaagat aaatttatct gtgatatagt caaaagtgca atggtagaaa
     5101 gtggagaagg attgattaaa aaatacaatt ttgactcaga agtgcactat aaatatattg
     5161 ataaaatttt aaatagattt aaaaatccat atcttaatga tgatgtatta agagtcggaa
     5221 gggaaccatt aagaaaacta agtgataaag atagattgat taaaccttta atgacagcaa
     5281 aatcatatgg attatcagtt gataatttga ttcttggaat aggtgcagcg cttcattata
     5341 ataatagtga agatactcaa agtgtagaat tacaagagtt gataaagtct attggggtaa
     5401 aaaaagctgt tgctaaaata gctaatataa gcaatgacga agaactttta aataatatcg
     5461 aaaaaagtta tatatttatg aaaaatttat aaatttactt gaaaaaaagt agttctgata
     5521 aagtaactat aatatattat tctacacagt ttgaattatt tgttgaataa tagagaatta
     5581 taatatataa ttaaaaggaa taaattaata tatttacctg tatatgtttg ataatatggt
     5641 tcaaatgttt ctactaagct accattaata gcttgactac aggagtgaag aagatatagt
     5701 ttatacttat tttaaattat atattaagtt aattactttg aattttaagc tcctgtctat
     5761 gatatagaca ggagcttttt tagtatttta atataaaata cttacaagaa tagatagcaa
     5821 aattagaaca aatataattt ttttattaat ataattttag aaagggctag gtggaagttc
     5881 aatgaaggct ttacaagaaa agatattgag agaaggcagt gtttcaggaa atgatatact
     5941 aaaagttgat agttttttaa accatcaaat tgatgtagct tttttaaatg aaataggaag
     6001 agaatttaaa gagagattta aaggagaaaa ggtggataag atatttacaa tagaggcttc
     6061 aggaatagct atagcatcaa ttgtttcaca gtattttgat aatgcacctg tagtatttgc
     6121 aaaaaaatca gaatctaaaa atttggataa agatgtatat gaaactaatg tatattcatt
     6181 tactaaggca agagagtatt cagttaaagt ttctaaaaag tacataaaca aaggcgaaaa
     6241 tatattgata gtggatgatt ttttggcaaa tggaagagct gctcttggac ttaaagattt
     6301 aattgagcaa gcagaagcta acttagttgg agtaggaatt gtcattgaaa aaggatttca
     6361 agctggagga gcacttttaa aggctaatga tgtaagatta gagtctttgg cagtagttga
     6421 atctatagat aatggaactg taaaatttag gtaatttatg atattatata cttgtgttta
     6481 gcacatattt taactatgta attatggagg attttttatg agatttttta tagacacagc
     6541 caatattgaa gaaataaaag aagcaaatga tttaggtgta atctgtggtg taactactaa
     6601 tccgtctcta atagctaaag aaggtagaga ttttatagag gtagttaaag aaataagtga
     6661 aatagtagat ggaccaataa gtgcagaagt aataagttta gaacataaag gtatgataga
     6721 ggaagcagaa aaattatcta agatacataa aaatatagtt ataaagatac ctatgacagc
     6781 agaaggtcta aaagcggtga aagtactatc aagtaaaggt ataaagacta atgtaacgct
     6841 tatattctct gctggacaag ctcttttagc tgctagagct ggagctacat atgtaagtcc
     6901 ttttgttggt agattggatg atatatcaca aaatggtctt gatttaattg aagaaatcgt
     6961 tgatatattt agtgtaaata gtattgaggc tcaaataata gttgctagtg ttagaaatcc
     7021 aatacatgtt ttacaagctg ctagaatggg agcagatata gcaacagttc ctcttaaagt
     7081 tataaaccaa atgattaagc atcctttgac agacaaagga atagatagct ttatgaagga
     7141 ttgggaaggt gcttctttaa aattataatt ataaactacg ctttataagt atattaaaga
     7201 ggttattatg gaaattctaa gtaataaggc taggctattt tgcctagtct tattttggtg
     7261 tataatatat tgaaacatat tattgaaata gattaatttt ttaagtgagg aggcgaaaaa
     7321 tggataaaat attaagagag atacaaagag aagataaaaa aaatccatat actgaccaag
     7381 aacttgctga aatgttaaat gtagctaggt ctgaagtgat atcagttagg aaaaaaaata
     7441 atattttaga ttcgagagaa agaagaaaaa aaatattaat aaaagatata tcaaaaattg
     7501 taagtgaaaa tcctcaaatg tctgaaagaa aaataactga aatattaata gaaaatggat
     7561 ataatatatc gagaagtgca gtttcaaaat tattaaaaga ggaaaattta aatcagtcaa
     7621 agattgggat taaggataaa gacccagcat catcaggaaa gtatgtgaag gtgagtaaag
     7681 ttgtagaaga agatggattt gaagaattga taggtataaa gggaagtttg aaagaaaagg
     7741 taaatctagc taagtcagca ataatgtatc ctcctaatgg ccttcatact ataatatatg
     7801 gagaaacagg agtaggtaaa agtgaattag ctacatgtat gtataaatat gctataaaaa
     7861 acaacattaa agaagagaac agcccattta tagtgtttaa ctgtgcagac tatgcagaaa
     7921 atcctaatct attgatagct caactctttg gggtagtaaa aggtgcatat acaggagcag
     7981 atgcaaatag agaaggtctt gtagaacaag ctaataatgg aatattgttt ttagatgaaa
     8041 ttcatagatt gcctgctgct gggcaagaaa tcttattttc attaatagat agaggagaat
     8101 ttagaaggtt aggagaaagt agttctaata gaaaggcaaa tgttctcata ataagtgcta
     8161 caacagaaaa tccagattct aatttacttc aaacatttag aagacgtata ccaatggtaa
     8221 taaatttacc taatatgagt gaaagaccaa aatctgaaag atatgaaata ataataaagt
     8281 tttttgaaag agaagcaaag cgagtaaaca agaattttat aataactaaa gaagttatgt
     8341 gtgcacttat gaattataag tgtactggaa atataggtca gttaaagagt gatatacaag
     8401 taacttgtgc tagagctttt agtaaatcga tattttctag cgataaggta attatagatt
     8461 tagatagttt aagggattat ataaaaagtg gatattatga ttgtaattgt aaaaatgaaa
     8521 agtattctga gttttcagtt gaggatatat atatagatat tagtgaattg gtgagtggaa
     8581 aaaatagaat tttatctgat aatgagataa gcgagatata taattatgca gaaaaagagc
     8641 ttaaggtttt ggaaagtaag tgcttttcag aagaagagtt aaaaaatgca tttatagaaa
     8701 agttagataa gaagtttaat gctattaaaa acaataaaaa tttatctgat agaagaagtc
     8761 gaatagactg gggaggacaa ttaaaagagt ctacactgga gataatggat aaggtaattg
     8821 tatttattaa ggagcagttt aagcatgtaa atcaaggtct gtacttggct ctagcaattc
     8881 atattgagca tgctataggt aggataaaag atggaaaaac tataataaat ccgagtttag
     8941 ataaaataaa agcatcaatg ccaatggaat atgagttgtc aagatatata atgggtatag
     9001 tagaagattt gacacaagta acatttccag aagatgagct tggatatttg gcttattata
     9061 taaataaatt ttgttataat gaggaaagta taaaagataa agttaaagta gtaatagtta
     9121 cgcatggtaa agttggaatt gaaatgtcta aagttgtaaa tcatatttta ggtatagaat
     9181 gtacactagg tatagaaata gcacttacag attcaccatc agaggggata gagcatgtat
     9241 tagaagagct tcaaaagata gaagctagaa aaggtattct agttctaatt gatatgggtt
     9301 ctttggtaat tcttggtgat gaggttgaaa aaaggcttgg aattagatgt aaaactgtaa
     9361 atagagtaga tacattattg gcaatggaag caggaaaact tgcaaccatt gaagggaaaa
     9421 gtttagatgg tataatagct gatttaaaga aaaataagaa ttatgctatg ataaatacaa
     9481 ataaatttag ttacagaaaa aacgagtatg gaaagaaaaa tgtaatcatt actttatgtt
     9541 tgagtggtgt aggtacagct ttaaatttaa aagagcatat agaaaaacaa attaaggaat
     9601 atgacacttt aattgaagta aagccagttg cttttttaaa taataattta gaagaagagt
     9661 taaatgatat tgaatctgaa tataatatta ttgctatctg tggaactatt gatatagatt
     9721 ataaaaacgt gccttttatt tcttataatg aggtacttgg taaaaatggt attaataaag
     9781 tattagagca tttaaatagt aaacaaccag ctttaaatat tgaaaaccag ttgaataatt
     9841 taatacatga agatttgatt ttatacaatt ttgaaggaat ttcaaaggag tatataattg
     9901 atactcttgt ttcaaaactt gaagaaggtg gatatgtcga ctcaaagtat atattaagtg
     9961 tatataaaag agaagcaatg ggaagtgttg taatggcctc taaggtagcc gtgccacatg
    10021 gacttccaga aaacgttata aagcctgcaa tagcaattgc gaggttgaat aaacctatag
    10081 tatgggacaa taaatttatg gtggatttgg tggtactact ggcacttaaa gaaaataata
    10141 agaaagaaat acgaagctta ttttcaaaga ttaatgacca gcatacttta gaagttttat
    10201 taaatactga agataaaaat gaaataaaag aacttttaag ctaattgtta gtaccgagaa
    10261 gaaagacaaa aaaatttgct ttagtataaa atttaaaact actcaattgc taagtag
//
LOCUS       DBGJFF010000136        10255 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.138, whole genome shotgun sequence.
ACCESSION   DBGJFF010000136 DBGJFF010000000
VERSION     DBGJFF010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10255
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.138"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            <1..606
                     /locus_tag="ACOIAF_003220"
     CDS             <1..606
                     /locus_tag="ACOIAF_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HGZ2670216.1"
                     /translation="DLADLGTVMGNVYHTKEEMIDCVNAFYEGMVQRSEQMKRHPNYK
                     TGENYAYLGLPPCFLIFDEYVAFFEMLGTKESVGLLSQLKKIVMLGRQAGYFLIVACQ
                     RPDAKYFSDGIRDNFNFRVGLGRISELGYGMLFGSDVKKQFFQKRIKGRGYCDVGTSV
                     ISEFYTPLVPKGHDFLQTIGFLAQARQDGTATCEAKGDGSD"
     gene            791..1987
                     /gene="mobT"
                     /locus_tag="ACOIAF_003221"
     CDS             791..1987
                     /gene="mobT"
                     /locus_tag="ACOIAF_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HGZ2670217.1"
                     /translation="MVLNEEQWIKELREKRIAYGISQGRLAVASGITREYLNKIESGK
                     MKPSKELLETLHKELARFNPEAPLTMLFDYVKIRFPTLDIQHIIKDILKLNINYMLHE
                     DYGHYSYTEHYSLGDIFIYTSADEEKGVLLELKGRGCRQFESYLLAQQRSWYDFLMDA
                     LIDGGIMKRIDLAINDHTGILDIPELAEKCRKREYIGKSRSYKYYQSGELIKHREDDR
                     EYMGSTLYLGSLKSDVYFCIYEKDYEQYVKLGTPLEEADIINRFEIRLRNERAYYAVR
                     DLLTYYDAEQTAFSIINQYVRFIDEEPDKRKNDWKLNDRWAWFIGDNRQSLKLTTKPE
                     PYTLDRTLRWVQRQVAPTLKMLKKIDKGNGTDYMETIEKQAILSEKHEMIIKQQTTPA
                     KDLVES"
     gene            2000..2134
                     /locus_tag="ACOIAF_003222"
     CDS             2000..2134
                     /locus_tag="ACOIAF_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017143882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HGZ2670218.1"
                     /translation="MWELVKDFLLVSMGMGIGVVLMCILSIGKEADYEMKQLEESEDN
                     "
     gene            2135..2356
                     /locus_tag="ACOIAF_003223"
     CDS             2135..2356
                     /locus_tag="ACOIAF_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670219.1"
                     /translation="MNFGQNLYQWFLSNAQSLVLMAIVVIGIYLGFKREFSKLIGFLV
                     VALIAVGLVFNAGGVKDVLLELFNKIIGA"
     gene            2395..3189
                     /locus_tag="ACOIAF_003224"
     CDS             2395..3189
                     /locus_tag="ACOIAF_003224"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670220.1"
                     /translation="MGTINYFLKGIKVLMISDEMRVDILHAVFINKNFIKDEENCNYE
                     KYLLELVNKSIYFREKSNFAEYVPPKSENHGECDCNSPNYQMDFKLLESTTRLHASKE
                     LTGQIQKFCDGVIGKCPPRRPNTQMTVTRLFASLRDYDCESLHSCLTEKYEYGTIEFD
                     IQTYVKLLTFKKNLFFFFPYKFSFNTCYNFKYALDSIRIALEKDFRESNLFREKYYAE
                     YDTFLAYIYEDNLIISKFEQDGKLQMIDCIYLFKSQTYSKLYEYTW"
     gene            3288..3584
                     /locus_tag="ACOIAF_003225"
     CDS             3288..3584
                     /locus_tag="ACOIAF_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005339688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HGZ2670221.1"
                     /translation="MNDIFKDMQAKVGCEYISDLPSYKRKVWHEMKRLNHADYEEKQL
                     EDFSKYVFGMSYQTLKDVMKQQKGREEQCRKQGYWWKREEQMAKKQHHTGLTYR"
     gene            3503..4006
                     /locus_tag="ACOIAF_003226"
     CDS             3503..4006
                     /locus_tag="ACOIAF_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HGZ2670222.1"
                     /translation="MQETRVLVETGRTNGEETTSYWFDLPIDVAEFEEKLGVGAESQD
                     YRIIEKVLPYADEVHEHTSVYQLNELDFMYRQLSADMQEEYVELLTVFENLEALYICR
                     NVITVYHDCKSMIDVARQKLMNDPTFKHLSEDCQAYYFDFEAYASHLLENKRYLVTEN
                     SIFELPE"
     gene            4024..4527
                     /locus_tag="ACOIAF_003227"
     CDS             4024..4527
                     /locus_tag="ACOIAF_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HGZ2670223.1"
                     /translation="MIDDMAVYIANLGRYNEGYLVGAWFTFPIDEEDVKEKIGLNEQY
                     EEYAVHDTDNFPIDIGEYVSIEELNEMYEMIEELPDYIVECLDEFISHYGTLEEVVEH
                     KDDIYFYPDCETMTDVAYYYIDELNALGDIPANLQNYIDYEAYGRDLDMGGRFIETSR
                     GICEIPY"
     gene            4645..5043
                     /locus_tag="ACOIAF_003228"
     CDS             4645..5043
                     /locus_tag="ACOIAF_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HGZ2670224.1"
                     /translation="MKKIKSYTGIWNVEKVLYAINDFNLPFPVTFTQITWFVITEFII
                     ILFGDMPPLSMIEGAFLKYFGIPVALTWFMSQKTFDGKKPYSFLKSQITYALRPKITY
                     AGKAVKLHKQILNETITAVRSVNYVPDKIY"
     gene            5021..7471
                     /locus_tag="ACOIAF_003229"
                     /pseudo
     CDS             5021..7471
                     /locus_tag="ACOIAF_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898619.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            7471..9675
                     /locus_tag="ACOIAF_003230"
     CDS             7471..9675
                     /locus_tag="ACOIAF_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HGZ2670225.1"
                     /translation="MKERIQSAFTKRKILHFLKMALFVVALSLILLSLLGTVAHATGL
                     VDDTINAENLYSKYPLSNYQLDFFVDNSWSWLPWNWLDGIGKSVQYGLYCITNFVWTI
                     SLYLSNATGYVVQEAYKLDFINDMADSIGKSIQTLAGVTENGFSSTGFYVGFLLLIIL
                     VVGMYVAYTGLIKRETSKALHAVINFVVVFVLSASFIAYAPDYIKKINEFSSDISTAS
                     LDLGTKIMLPNSDSEGKDSVDLIRDSLFSIQVQQPWLLLQFGNSNAEEIGTDRVEALV
                     SASPEDEDGKTREEVVKTEIEDNDNNNLTIPQVVNRLGMVFFLLFFNLGITIFVFLLT
                     GMMLFSQILFIIFAMFLPISFLLSMIPSYESMAKQAIVRVFNTIMTRAGITLIVTVAF
                     SISSMFYNISTDYPFFMVAFLQIVCFAGIYMKLGDLMSMFSLNAGDSQSMGRRIFRRP
                     YLFMRHRARRMEHRIARAVSAGGISGGVAGAVVGSAVAGKRAERKNTASKENRGNTTS
                     SMGQRAGSKVGAVLDTKNKVKDKANAVKENIKDMPTQTAYAVYSAKEKAKSSVSDFKR
                     GMVQEQQSRQTGRLEKQEQHKKSIAVKRMELQKAQEARQAQRKADGSATTGATRPHER
                     PATASTVSKTGAEKMQEVKRPATTPTTKASEPVKTSGVKERPLSSSTPDGKATQSTYR
                     QNVEKVTVSKETRQNYTKDRRTKVQQTQTVQKNQQTTEKIRNLVTKKGQKKK"
     gene            9672..>10255
                     /locus_tag="ACOIAF_003231"
     CDS             9672..>10255
                     /locus_tag="ACOIAF_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HGZ2670226.1"
                     /translation="MKLKHIAIIGSLFPILFSLVLFFGVLISADSDDENSNFSSGITG
                     MNLSAEVLKHQPMVEKYARENGISEYVNVLLAIIQVESGGTAEDVMQSSESLGLPPSS
                     LDTESSIKQGCKYFASLLSSCKNQDIDDLNVAIQSYNYGGGYVGYVAGNGKKHTFNLA
                     ESFAREKSGGKKVTYTNPIAVAKNGGWRYGYGNM"
BASE COUNT         3203 a         1917 c         2414 g         2721 t
ORIGIN      
        1 gacttagcag acttgggaac agtcatggga aacgtgtatc acaccaaaga agaaatgata
       61 gactgtgtca atgcttttta tgagggcatg gtacagcgaa gcgagcaaat gaaacgacac
      121 cctaactata agacgggcga aaattacgcc tatctgggac tgccaccctg ctttcttatc
      181 tttgatgaat atgtggcgtt ttttgaaatg ctggggacaa aagaaagtgt gggcttactt
      241 agccagttaa agaaaatcgt catgttagga cgacaagcag gttatttcct tatcgttgcc
      301 tgccagcgtc cagacgcaaa atatttctcg gacggtatca gagataactt caatttccgt
      361 gtgggattgg ggcgtatcag cgagttaggt tacggtatgc tgtttggttc agatgtgaaa
      421 aagcagtttt tccaaaagcg tatcaaaggg cgtggctatt gtgatgtggg aacaagcgtc
      481 ataagtgaat tttacacgcc tttagtcccg aaaggacatg attttttgca gacaatcggt
      541 tttcttgcac aagcaaggca ggacgggacg gcgacgtgcg aagcgaaagg cgacggctcg
      601 gactagccgt tgctggtgtg gcgtaagcca cgccagcagg taaaacccct cggtatctaa
      661 cagaggggta cgtttgcaaa tagacaatag gcaaaaaaac ataggaaaca taaggcttct
      721 ggcatggttt cctagcaaaa atcggctgtg aagtcgcctt aaaaaacttc acacttgaca
      781 gattgggggt atggttctga atgaagaaca atggataaaa gaattacggg agaagcggat
      841 tgcttacggt atctcacaag gcaggctggc ggtagcttct ggtatcacaa gggaatatct
      901 aaacaagata gaaagcggaa aaatgaagcc gtcaaaagaa cttctggaaa ctttgcataa
      961 agaactggca aggttcaatc cagaagcacc gcttaccatg ctgtttgatt atgtaaaaat
     1021 tcgttttccc acgctggata tacagcacat catcaaagac atattaaagc tgaatatcaa
     1081 ttatatgctc catgaagatt acggacatta cagttatacg gagcattatt cattagggga
     1141 catctttatc tatacgtcgg ctgacgaaga aaaaggtgtc cttttagagt taaagggtcg
     1201 gggttgcagg cagtttgaaa gttacctgct ggcacagcaa agaagctggt atgactttct
     1261 tatggacgca ctcatagacg gaggcattat gaagcgtatc gaccttgcta tcaacgacca
     1321 tacgggcatt ttggatattc cagagcttgc ggagaaatgc aggaaacggg aatatatcgg
     1381 aaagtccaga agctataagt attaccagtc gggcgagctt atcaagcaca gagaggacga
     1441 cagagaatat atggggagta ccctttatct tggttcgctg aaatcagatg tgtatttctg
     1501 tatctatgaa aaggactatg agcagtatgt caaattaggt acacctctgg aagaagccga
     1561 cattatcaac cgttttgaga tacggcttag aaatgaacgt gcttactatg cagtacgaga
     1621 tttgctgacc tactatgacg cagagcagac cgctttttct atcatcaacc agtatgtgcg
     1681 gtttattgat gaagaaccag acaagcggaa aaatgactgg aaactcaatg accgctgggc
     1741 ttggtttatt ggcgataatc ggcaaagtct gaaactgacg acaaaaccag aaccttacac
     1801 cttagaccgt acattgcggt gggtacaaag acaggtagca ccgaccttga aaatgctgaa
     1861 aaagattgat aagggaaacg gtacagacta catggaaacg attgaaaagc aggcaatctt
     1921 gagtgaaaag catgaaatga taatcaaaca gcagacgacc cctgcaaaag atttggtgga
     1981 aagttaggag tgataaaaaa tgtgggaatt agtaaaagac ttcctgctgg tatctatggg
     2041 aatgggtatc ggcgtagtct tgatgtgtat tttgagtatc ggcaaagaag ctgactatga
     2101 aatgaaacaa ttagaagaaa gcgaggacaa ttaaatgaat ttcggacaaa atttgtatca
     2161 atggttttta tcaaatgcac agagcttagt gcttatggcg attgtggtta tcggtatcta
     2221 cttagggttc aagcgtgagt tttcaaaact tattggcttc ttggtagttg cccttattgc
     2281 tgtcggctta gtattcaacg ctggcggtgt gaaagatgta ctgttagagc tgttcaataa
     2341 gattattgga gcataacgaa ttttattgtt gctgtaatat gagaatgtta taatgtgggt
     2401 acgataaatt attttttgaa aggaataaaa gtgcttatga tttcagatga aatgagagtg
     2461 gatattttac atgcagtatt cataaacaaa aattttatta aagatgaaga aaattgtaat
     2521 tatgaaaaat acttgttgga attagttaat aaatcaatat attttagaga aaaatcaaat
     2581 tttgcagaat atgtaccacc taaatctgaa aatcatggag aatgtgattg taactcacct
     2641 aattatcaaa tggactttaa attacttgaa agcacaacaa gacttcatgc ttcaaaagaa
     2701 cttactggtc aaatacagaa attctgtgat ggtgtaattg gtaagtgtcc accgagaaga
     2761 ccaaatacac aaatgactgt aactaggtta tttgcttcgt tgcgggatta tgattgtgaa
     2821 agtttacatt cttgtcttac agagaaatat gagtatggaa cgattgaatt tgatatacaa
     2881 acctatgtga aattgcttac tttcaagaaa aacttatttt tcttttttcc atacaagttt
     2941 agttttaata cttgttataa ctttaaatat gctttagaca gtataagaat tgctttagaa
     3001 aaagatttta gagagtcaaa tctttttaga gaaaaatact atgcagagta tgatactttc
     3061 ttagcatata tctatgaaga taatctgata ataagtaaat ttgaacaaga cggcaagtta
     3121 caaatgattg attgtatata tttatttaaa agtcaaacat acagtaaact ttacgagtac
     3181 acatggtaaa tcagttacac ttaaacaaat tcatacttac acacaaagca gttagtctta
     3241 ggattgactg cttttttctt acctaaattt cagattggag tgatgaaatg aacgatattt
     3301 ttaaagatat gcaggcaaaa gtcggctgtg aatacatttc cgacctgccc tcttacaagc
     3361 gtaaggtgtg gcatgaaatg aaacggctga accatgccga ctatgaagaa aaacagttgg
     3421 aagatttttc taaatatgtg tttggtatgt cataccagac cttaaaagat gtgatgaaac
     3481 aacagaaagg acgtgaggaa caatgcagga aacaagggta ctggtggaaa cgggaagaac
     3541 aaatggcgaa gaaacaacat catactggtt tgacttaccg atagatgttg cagagtttga
     3601 agaaaagtta ggtgtcggtg cagaaagtca agattaccgc attatcgaaa aggtgttgcc
     3661 ttatgctgat gaagtccacg aacacaccag cgtgtaccag cttaacgaat tagattttat
     3721 gtaccgccag ctatcagccg atatgcagga agaatatgta gaacttctta ccgtgtttga
     3781 gaacttagaa gcactttata tttgcaggaa cgtgattacc gtttatcacg actgtaaaag
     3841 catgatagat gttgcaaggc aaaagctgat gaacgacccg acattcaaac atttatccga
     3901 ggactgtcaa gcatactatt tcgattttga agcctacgct tctcacttac ttgaaaacaa
     3961 acgctatctt gtaacggaaa acagtatctt tgaactgcca gaataggaaa tgaggtggtg
     4021 cttatgattg atgatatggc ggtttatatt gctaatcttg gcagatacaa tgagggatat
     4081 ttagtcggtg cgtggttcac tttccctatt gacgaggaag atgtaaaaga aaaaatcggc
     4141 ttgaatgaac agtacgaaga atacgctgtc catgataccg acaactttcc tatcgacata
     4201 ggggaatatg tttccattga agaactcaat gaaatgtatg aaatgataga ggaacttccc
     4261 gactatatcg tagagtgtct ggacgaattt atcagccact atgggacgct ggaagaagtc
     4321 gtggaacata aggacgatat ttatttttat cccgattgtg aaaccatgac agatgttgcc
     4381 tactactaca tagacgaatt gaacgcactt ggggacattc cagctaactt acagaactat
     4441 attgactatg aagcctacgg gcgagatttg gatatgggtg gtcgattcat tgaaacaagc
     4501 cgaggtatct gtgagatacc atattaacgc tggaagatca gcaacaagca ttgttgctac
     4561 tgacagcgta tttctcttta agggacagtc tgaaaatggc tgtccttttc tctttgaaga
     4621 acttacgaaa ggggctgaaa gaacttgaaa aagattaaaa gctacacggg tatctggaac
     4681 gtggaaaaag tcttatatgc aatcaatgac tttaacttac cctttcccgt tacctttaca
     4741 cagattacat ggtttgtgat tacggaattt atcatcattc tgtttgggga tatgccccca
     4801 ctttccatga ttgagggagc atttctcaaa tacttcggta ttcccgttgc tctcacttgg
     4861 tttatgtcgc agaaaacctt tgacggaaag aagccgtaca gctttttgaa atcacagata
     4921 acctatgcct tgcgacctaa aatcacttat gcaggaaaag ccgtaaaact gcataagcag
     4981 atattgaatg aaacaatcac ggcagtaagg agtgtgaact atgttcccga taaaatatat
     5041 tgacaataac cttgtctgga acaaggacaa tgaggtgttc gcctactatg agctgatacc
     5101 gtacaactat tcttttctat ccgcagagca gaaatttatc gtgcatgaca gttttcgaca
     5161 gctaatcgca cagtcccgtg agggaaaaat tcatgcgttg cagattgcca cagaaagctc
     5221 cattagaagt atgcaggagc agtcaaagaa actggtaacg ggcaaacttc gtgaggtggc
     5281 agtctaaaaa atagacgaac agaccgaagc gttagtatca atgattgggg acaatcaagt
     5341 ggactaccgc ttttttcttg gctttaagct catggttacg gaagaacagc tcaatctgaa
     5401 gaacatcaaa aaatcggcat ggctgacgtt caaggaattt cttcatgaag tgaaccacac
     5461 gctgatgaat gattttgttt ccatgccgaa tgatgaaatc aaccgttaca tgaaaatgga
     5521 aaagttattg gaaaataaaa tctcccgtcg ctttaaggtg cgtcgcttgg aaatccatga
     5581 ttttgggtat ctcatggaac atctttacgg cagggacggt atcgcctatg aagattatga
     5641 gtaccagcta ccaaagaaga aattgaagaa agaaacgctg ataaaatact acgaccttat
     5701 ccgtccgaca aggtgtgtga ttgaggaaag ccagcggtat ttacgattgg aacatgagga
     5761 caaggaaagc tatgtgtcct attttaccgt caatgtaatt gtcggggagt tggattttcc
     5821 gtcctctgaa atcttctatt tccagcaaca gcaattcaca ttccccgttg atacttctat
     5881 gaatgtagaa atcgtggaga accgtaaggc attaacgaca gtccgcaaca agaaaaagga
     5941 attgaaagat ttggataatc acgcctatca agcaggaagt gaaaccagct caaatgtggt
     6001 ggacgcttta gacagcgtgg acgagctgga aaccgattta gaccagagca aagaaagtat
     6061 gtataagtta agctacgtca tacgggtgtc tgcacccgat cttgacgagc tgaaacgccg
     6121 ttgtgatgaa gtcaaggact tttacgacga cctcaatgta aaactggtgc gtcctgctgg
     6181 cgatatgttg ggacttcatt ctgaattttt acctgccagc aagcgatata tcaatgacta
     6241 tgtgcagtat gtaaagtcag acttcttggc tgggcttggt tttggtgcga cccagcagtt
     6301 aggggaaaac acgggtatct atatgggcta ttccgttgat acgggaagaa atgtgtacct
     6361 gcaaccgtct ttagcttcgc agggagtaaa aggcacagtt actaatgccc ttgcttctgc
     6421 ttttgtggga agtcttggcg gtggaaagtc attctgcaac aatctacttg tatattatgc
     6481 ggtgctgttt ggcggtcaag cacttctgtt agaccctaaa agtgagcgtg gcaactggaa
     6541 agaaacgctc ccagagatag cccatgaaat caatatcgta aaccttacca gcgacaagga
     6601 caatgcagga cttcttgacc catttgtgat tatgaagaat gtgaaagacg ctgaaagtct
     6661 ggcaatcgac atcttaacat tccttacggg tatttcctct agggacggcg aaaaattccc
     6721 cgtgttacga aaagcggtgc gttctgttac ccagagtgaa aatagaggac tgttacacgt
     6781 tattgaggaa ttgagaaaag aagataccgc tatctcacgc aatatcgcag accatatcga
     6841 cagcttcacg gactacgact ttgcacacct gctgttttca gacgggacgg tagaaaatgc
     6901 tataagcctt gataaccagc tcaatatcat tcaagtagca gaccttgtac tgccagataa
     6961 agataccact tttgaggaat acacgaccat tgaattattg tcggtgtcta tgctgattgt
     7021 gattagtacc tttgcccttg attttatcca ttcggacaga agcattttta agattgtaga
     7081 tttggacgaa gcgtgggcgt tcttaaatgt ggcacaaggc gaaaccctat caaataagct
     7141 ggttcgtgct ggacgagcta tgcaggcagg cgtttatttc gttacccagt ctgcctatga
     7201 cgtgtcaaag gaaagtctga aaaacaatat cggtttgaag tttgcatttc gttctactga
     7261 catcaacgag ataaagcaga ccttagaatt tttcggtatc gacaaggacg acgaaaacaa
     7321 ccagaaacgc ttgcgtgatt tggagaacgg acaatgttta ttgcaggact tatacgggcg
     7381 tgtcggtgtg gtgcagatac acccagtctt tgaagaatta ctacacgcct ttgatactag
     7441 accgcccgta cagagaaatg aggtggagtg atgaaagaaa ggatacagag tgcgttcaca
     7501 aaaaggaaga tacttcactt tctcaaaatg gctctgttcg tggtggcact ttcccttatc
     7561 ctgctttcac ttctggggac ggtggctcat gcgacggggc ttgtggacga taccataaac
     7621 gcagaaaatc tatattcaaa atatccgctt tccaactacc agcttgattt tttcgtcgat
     7681 aatagctggt cgtggcttcc gtggaactgg ctggacggta tcggaaaatc ggtacagtac
     7741 gggctttact gcattaccaa ctttgtctgg acgataagcc tttatttaag caatgcaact
     7801 ggctatgtgg tgcaggaagc ctataaactt gattttatta acgatatggc agacagtatc
     7861 ggaaaaagca tacagaccct tgcaggcgtt accgagaacg gcttttcttc tacgggcttc
     7921 tatgttggtt tcctgcttct tattatctta gtggtgggaa tgtatgttgc ctatacggga
     7981 ctgataaaac gggaaaccag caaggcactt cacgctgtta tcaactttgt ggtggtgttc
     8041 gtgctgtccg cttcgtttat tgcctacgct cccgactaca tcaagaagat aaatgaattt
     8101 tcatcagaca tcagtaccgc ttcgcttgat ttgggaacaa aaatcatgct ccccaactct
     8161 gacagcgagg gcaaggacag cgtagacttg atacgggaca gcctattttc tattcaagta
     8221 caacagccgt ggctactctt acagtttgga aacagcaacg cagaggaaat cgggacagac
     8281 cgtgtcgaag ctctcgtatc ggcaagtcca gaggacgagg acggaaagac cagagaagaa
     8341 gtcgtgaaaa cagaaatcga ggacaacgac aacaacaatc tgacgatacc gcaggtggta
     8401 aaccgtttag gtatggtgtt cttcctcttg ttctttaatc ttggtattac gatatttgta
     8461 ttcttgctta cgggtatgat gttgttcagc cagatacttt ttattatctt cgctatgttt
     8521 ttacctatca gctttctact ttccatgata ccgagctatg aaagcatggc aaagcaggca
     8581 atcgtgaggg tatttaatac cataatgaca cgggcaggaa taacgctcat tgtaacggtt
     8641 gcgtttagca tttccagtat gttttataac atatccacgg actatccgtt tttcatggtg
     8701 gcgttcttgc agatagtatg tttcgctggt atctatatga agctgggcga cttaatgagt
     8761 atgttttctc tcaacgctgg cgacagtcaa agcatgggac gcaggatatt ccgcagaccg
     8821 tatctgttca tgcgacatcg ggctaggcgt atggaacacc gtattgcaag ggctgtaagt
     8881 gctggcggta tttcgggcgg tgtggctggt gcggtagttg gaagtgccgt tgctggcaag
     8941 agagccgaaa gaaaaaatac ggcttctaaa gaaaatcggg gcaataccac ttccagtatg
     9001 ggacagcgtg caggctcaaa ggtgggtgct gtcttagata cgaaaaataa agtgaaagac
     9061 aaggcaaacg ctgtcaaaga gaatatcaag gatatgccca cacagaccgc ttatgcggtg
     9121 tattccgcaa aggaaaaggc aaagtccagc gtgtctgatt tcaagcgtgg tatggtgcag
     9181 gaacagcagt ccagacagac gggacgcttg gaaaagcagg aacagcataa gaaaagtatc
     9241 gctgtcaagc gtatggagct tcaaaaggca caagaagcaa ggcaggcaca gcgaaaggct
     9301 gacggatcag cgacaacggg agctacccgt ccccatgagc gaccagccac agcttcaacc
     9361 gtttcaaaga cgggtgcgga aaaaatgcag gaagtcaaac gccctgccac aactccgact
     9421 acgaaagcaa gcgaaccagt taagacaagc ggtgtcaaag aacgtccgct atcttctagt
     9481 actcctgacg ggaaagcgac ccaatcgaca tataggcaga atgtagaaaa agttaccgtg
     9541 tcaaaggaaa cacgccagaa ttacaccaaa gaccgtagga caaaggttca gcagacccag
     9601 actgtccaga agaaccagca gaccacagag aaaatccgta atcttgtaac gaagaaagga
     9661 cagaagaaaa aatgaaactg aaacatatcg ctatcattgg aagtctgttt cctattctct
     9721 tttctctggt gcttttcttt ggggtgctga ttagtgcgga cagcgacgac gaaaacagca
     9781 acttttcttc tggcattacg ggtatgaact tatccgcaga agttttgaaa catcagccta
     9841 tggtagaaaa atacgccaga gaaaacggta tctccgagta cgtcaatgta ctactggcta
     9901 tcattcaagt agaaagtggc ggtacggcag aagatgttat gcagagttca gaaagtcttg
     9961 gtttaccgcc tagttcctta gatacggaaa gctcaatcaa gcaggggtgt aagtattttg
    10021 cgtccctgct ttcttcctgc aaaaatcaag atatcgacga tttgaatgta gcgatacagt
    10081 cctataacta tggcggtggc tatgtgggct atgtggcagg aaacggaaag aaacacactt
    10141 ttaatcttgc agagagcttc gcccgtgaga aatcgggtgg aaagaaagta acctatacca
    10201 acccgatagc cgttgcgaag aacggaggct ggcggtatgg ctatggaaat atgtt
//
LOCUS       DBGJFF010000137        10003 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.139, whole genome shotgun sequence.
ACCESSION   DBGJFF010000137 DBGJFF010000000
VERSION     DBGJFF010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10003
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.139"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            <1..1010
                     /locus_tag="ACOIAF_003232"
     CDS             <1..1010
                     /locus_tag="ACOIAF_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HGZ2670227.1"
                     /translation="LDDKGYYFNSNWKDIHIGIYDTKLGDSLKYITNEYIENMESVKY
                     DYRELKNIENQTDGMCILAIRQNYKALEYVKNQTEELCLEAIKYNYKALEYVKEQTEY
                     LCLEAIKKDCNALKYVRNKTEGLIIKAISHSSNIDVVSILKALETQTRRICLEAIKKD
                     GRCLAYVREQSEELCIEAIKQNYKALKYVKNQTEKMCIESVRQNGMALQYVNKQTDKI
                     CIEAVKQDGRSIQFVNNKTEEICINAIRYLNKKYNIKDVLSYIDKYTEDICIEIVRQN
                     GKMLMYIKNQTEKMCIEAVKENYKSLKYVKEQSERICKEALKQNHKAKEYVKIAIDDC
                     I"
     gene            1149..1601
                     /gene="tsaE"
                     /locus_tag="ACOIAF_003233"
     CDS             1149..1601
                     /gene="tsaE"
                     /locus_tag="ACOIAF_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HGZ2670228.1"
                     /translation="MAKIYLENENKTREIGYKLGKLLKEGSVICLVGDLGAGKTTMTQ
                     SLADSLGIEDYITSPTFTIINEYEGKIPLYHFDVYRIGSSDEMYDIGYDEYVNSNGIC
                     IIEWANLIEDILPKEYLNIELRYKDEGREMILTPKGEFYKEIVEELIK"
     gene            1601..2317
                     /gene="tsaB"
                     /locus_tag="ACOIAF_003234"
     CDS             1601..2317
                     /gene="tsaB"
                     /locus_tag="ACOIAF_003234"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HGZ2670229.1"
                     /translation="MKILGMDTSSMAASVAVVEDDNLICEFTVNNKKTHSQKLMPMIE
                     NMLSMSDLSIKDMDLLAVCIGPGSFTGLRIGMATVKAMAHVNNIPIIAVNSLESLANN
                     INFCDRKICCILDAQKNQVYSAKYKFENGEIIELDGVDVVEFDALVDEIVSTNEEFII
                     VGEAVYKYKDKLENIKNIKIPSPANNVSNAGSLCSLALNKYNKNIDVHTCYTINPMYI
                     RKSQAEVQYDEKMKRLNNGR"
     gene            2307..2783
                     /gene="rimI"
                     /locus_tag="ACOIAF_003235"
     CDS             2307..2783
                     /gene="rimI"
                     /locus_tag="ACOIAF_003235"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HGZ2670230.1"
                     /translation="MEDNTKLIVDDIKIEEMTTEDIDEVFEVEKNCFEDYWSKESFRK
                     ELSNEVARYIVAKLNGKIVGYVGIWLILDEGHINNVAVHSDYRGKKIGDKLIKGIVDL
                     CKDNNIASMTLEVRASNKIAQNLYRKYGFKMGGIRKEYYSDNKEDAIIMWNQLKEV"
     gene            2789..3805
                     /gene="tsaD"
                     /locus_tag="ACOIAF_003236"
     CDS             2789..3805
                     /gene="tsaD"
                     /locus_tag="ACOIAF_003236"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HGZ2670231.1"
                     /translation="MSDIITLAIESSCDETAASVLKNGREVLSNIISTQIETHKKFGG
                     VVPEVASRKHVENIDIVVQEALDKANIGFNDIDHIAVTYGPGLVGALLVGLSYAKALA
                     YTLNIPLVGVNHIEGHLSANYIEHKDLKPPFITLIVSGGHTHLVEVKDYGKYEILGKT
                     RDDASGEAFDKISRAMNLGYPGGPIIDNLAKNGNKHAIEFPRAYLEEDSYDFSFSGLK
                     SSVLNYLNGKRMKNEEIVVEDVAASFQEAVVEVLSTKALKAVKDKGYNIITLSGGVAS
                     NSGLRAKITELAKDNGITVKYPPLILCTDNAAMIGCAGYYNFINGKTHDMSLNAVPNL
                     KINQ"
     gene            4102..6810
                     /gene="hpdB"
                     /locus_tag="ACOIAF_003237"
     CDS             4102..6810
                     /gene="hpdB"
                     /locus_tag="ACOIAF_003237"
                     /EC_number="4.1.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase large
                     subunit"
                     /protein_id="HGZ2670232.1"
                     /translation="MSQSKEDKIRSILEAKNIKSNFQNKENLSEFNEKKASKRAEDLL
                     DVYYNTLSTADMEFPYWYNREYRKSDGDIPVVRRAKALKAAFSHMTPNIIPGEKIVMQ
                     KTRHYRGSFPMPWVSESFFVAQGEQMREEAKKLASNTADELTKFGSGGGNVTESFGNV
                     VSIAGKFGMRKEEVPVLVKMAKEWVGKSVEDLGFHYEKMMPDYDLKENLMSTLICMFD
                     SGYTLPQGREVINYFYPLNYGLDGIIEMAKECKKAVAGNASGDGLIGMDRLYFYEAVI
                     QVIEGLQTWILNYAKHAKYLESIETDLEAKKEYSDLVEILEHIAHKQPRTFREALQLT
                     YTIHIASVNEDAISGMSIGRFGQILYPWYEQDIEKGLITKEEVIELLELYRIKITCID
                     CFASAGVNGGVLSGNTFNTLSIGGLKEDGSTGANELEELLLEASMRCRTPQPSLTMLY
                     DEKLPEDFLMKAAECTKLGSGYPAWVNNSNGTTFMMKQFADEGMTVEEARAFALGGCL
                     ETSPGCWKQLTLNGKTYSIAGGAGQSAGSGVHFIANPKILELVLMNGKDYRMNIQVFE
                     PHNKPLDTYEEVIEVFKDYYKQAINVLERANNIELDIWRKFDTSIINSLLKPDCLDKG
                     QHIGNMGYRYNATLNVETCGTVTMVNSFAALKKLVYDDKAFTIEEMKDAILNNFGFKD
                     ALEVGNYSMADQVKVDKTGKYDAIYKACLDAPKYGNNDLYADNILKNYEVWLSKVCEE
                     AQSLYAKKMYPCQISVSTHGPQGAATLATPDGRLSGTTYSDGSVSAYAGTDKNGVYAL
                     FESATIWDQAVVQNSQMNLKLHPTTIKGQQGTKKLLDLTRSYLRKGGFHIQYNVVDSE
                     TLKDAQKNPDNYRQLMVRVAGFTQYWCELGKPIQDEVIARTEYEGV"
     gene            6814..7071
                     /gene="hpdC"
                     /locus_tag="ACOIAF_003238"
     CDS             6814..7071
                     /gene="hpdC"
                     /locus_tag="ACOIAF_003238"
                     /EC_number="4.1.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase small
                     subunit"
                     /protein_id="HGZ2670233.1"
                     /translation="MRKHSDCMNFCAVDATKGICRLSKQMINLDDAACPEIKVMPKCK
                     NCKNFVEANDEGIGKCVGLEKEDWVYSTLNAITCEGHVFNE"
     gene            7064..8014
                     /gene="hpdA"
                     /locus_tag="ACOIAF_003239"
     CDS             7064..8014
                     /gene="hpdA"
                     /locus_tag="ACOIAF_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase activase"
                     /protein_id="HGZ2670234.1"
                     /translation="MSSQKQLEGMIFDVQSFSVHDGPGCRTTVFLNGCPLSCKWCANP
                     ESWTVRPHMMFSELSCQYENGCTVCHGKCKNGALSFNLDNKPVIDWNICKDCESFECV
                     NSCYYNAFKLCAKPYTVDELVQVIKRDSNNWRSNGGVTFSGGEPLLQHEFLHEVLLKC
                     HEVNIHTAIETSACVSNEVFNKIFNDIDFAFIDIKHMDREKHKEQTGVYNDLILENIS
                     NLANSDWNGRLVLRVPVISGFNDSDENISDIISFMHKNNLVEINLLPFHRLGESKWTQ
                     LGKEYEYSDKGDVDEGHLEELQDIFLDNGIACYVGHETAF"
     gene            8329..9291
                     /locus_tag="ACOIAF_003240"
     CDS             8329..9291
                     /locus_tag="ACOIAF_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HGZ2670235.1"
                     /translation="MNKSWSNLIIKNKDRKVRSGWIIIAVMATFYILQYYLSMILVEI
                     IRKILVATGDINLKTNYFSEYANWLNDVGLQIVFQILTDSLMIIIPIIVWRFIMKNPI
                     SEMGLHSYRIKKKEGYVGMLLGIFNCTVIFIITITIGGGHVTSWVPKISPLTVSWILV
                     FILVAFAEEILNRGFFMAVLRRCKNIYFIMIVPSVIFGLIHIWNPDVTFLSVINIIII
                     GILFSYMFIKSSNIWMCIGYHFAWNVFQGIIYGMPVSGLQVPGIITTQITHGNLLNGG
                     MFGIEGGILTTFVNLLSFIFVWYYYRDSKYDFVSDKVNVTNIKN"
     gene            complement(9390..9830)
                     /locus_tag="ACOIAF_003241"
     CDS             complement(9390..9830)
                     /locus_tag="ACOIAF_003241"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HGZ2670236.1"
                     /translation="MYIYSNNIINYYLYFFSEEYAWRGFLQNIFFDKFGKKLGVIILG
                     MCWSLWHLPLIFTLYTPEAPILGIILRSIHIVGISIFLGYLYIKTKNIWFCAIIHVLN
                     NSAFLITNSKESTITYHDILIVSMVVLIFYVPFLFTKEYKNNLE"
     gene            complement(9716..>10003)
                     /locus_tag="ACOIAF_003242"
     CDS             complement(9716..>10003)
                     /locus_tag="ACOIAF_003242"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
                     /protein_id="HGZ2670237.1"
                     /translation="ISSLSIIIYSSNKKNLYPVKNIKLGSLLIIYFMISVIVPTSFIL
                     ILQGAQPNYSGIIKFIFTPITSLTTIYIFLVKNMPGEVFYRIFFLINLVKS"
BASE COUNT         3912 a         1046 c         1858 g         3187 t
ORIGIN      
        1 ttttagatga taaagggtat tattttaata gtaattggaa agatattcat attggtattt
       61 atgatacaaa attaggagat tctttaaagt atatcactaa tgaatatatt gaaaatatgg
      121 aaagtgtaaa atacgattat cgtgaattaa aaaatataga aaatcaaact gatggtatgt
      181 gtattttagc aataaggcag aattacaagg cattagagta tgtaaaaaat caaaccgaag
      241 aattatgttt agaagcaata aagtataatt ataaagcact tgaatatgta aaagaacaaa
      301 cagaatactt gtgtcttgaa gctataaaaa aagattgtaa tgcacttaag tatgtacgaa
      361 ataaaacaga agggcttatt ataaaagcaa taagtcattc ttccaatata gatgttgtaa
      421 gtatactaaa ggctttagag actcaaacaa gaaggatatg tttagaagca attaaaaaag
      481 atggcaggtg tcttgcatac gttagagaac agagtgaaga attatgtata gaggccatta
      541 agcaaaatta taaagcatta aaatatgtaa aaaatcaaac ggaaaaaatg tgtatagaat
      601 ccgtaagaca aaatggtatg gcattacaat atgtaaataa acaaactgat aaaatatgta
      661 tagaagctgt caaacaagat ggaagaagta tacaatttgt caacaataag acagaagaaa
      721 tatgtataaa tgctataaga tatttaaata aaaaatataa tattaaagat gtactaagtt
      781 acatagataa gtatacagaa gatatatgta tagagattgt aagacaaaat ggaaaaatgc
      841 ttatgtatat aaaaaatcaa acagaaaaaa tgtgtattga agcagtaaaa gagaactata
      901 agtctcttaa gtatgtaaaa gagcaatctg agagaatatg taaagaagca ttgaagcaaa
      961 accataaagc aaaagagtat gtaaaaattg ctattgatga ttgtatataa ggtgtagata
     1021 cagccatcaa tagtgaaaga taaaaaaaga taaagacaag tactttataa taatctaggc
     1081 ttatactata attaattaag cagtgatata aattcacatg acgattatat caaaatggag
     1141 atgaattgat ggcaaaaata tatttagaaa atgaaaataa aactagagaa ataggatata
     1201 aattaggtaa acttttaaaa gaaggcagtg ttatctgttt agtaggagat ttaggagctg
     1261 gtaaaactac aatgacacaa agtttggcag attcattagg gatagaagat tatataacaa
     1321 gtcctacttt tactataata aatgaatatg aaggaaaaat acctttgtat cattttgatg
     1381 tttatagaat aggtagtagt gatgaaatgt atgatattgg ttatgatgaa tatgtaaatt
     1441 caaatggaat ctgtataata gaatgggcta atctaataga agatatactt ccaaaagaat
     1501 atttgaatat agaattgaga tataaagatg aaggaagaga aatgatatta actccaaaag
     1561 gagagtttta taaagagata gttgaggagt tgattaaata atgaaaatac taggtatgga
     1621 tacgtcttct atggcagcaa gtgtagcagt tgttgaagat gataacttaa tatgtgagtt
     1681 tacagtaaac aacaaaaaaa ctcattcaca aaagcttatg ccaatgatag aaaatatgtt
     1741 gagtatgagt gatttaagta taaaagatat ggatttatta gctgtatgta taggtccagg
     1801 ttcgtttaca ggacttagaa taggtatggc tacagtaaaa gctatggccc atgtaaataa
     1861 tataccaata atagcagtaa attctttgga atctctagca aataacataa atttctgtga
     1921 tagaaagata tgttgtatat tagatgcaca gaaaaatcaa gtttattcag ctaaatacaa
     1981 gtttgaaaat ggagagataa tagaattaga tggtgttgat gttgtagagt ttgatgcttt
     2041 agtagatgaa attgtatcaa ctaatgaaga atttataata gttggagaag ctgtatacaa
     2101 atataaagac aagttagaga atataaaaaa tataaaaata ccttcaccag ctaataatgt
     2161 atctaacgca ggaagcttat gctctttagc attaaataaa tataataaaa atatagatgt
     2221 acatacttgt tatacgataa atcctatgta tatcagaaaa tctcaagctg aagtacaata
     2281 tgatgaaaaa atgaagaggc taaataatgg aagataatac aaaactaata gtagatgata
     2341 taaaaataga agaaatgact acagaagata tagatgaagt ttttgaagta gaaaaaaatt
     2401 gttttgaaga ttattggtca aaagagtctt ttagaaaaga gttgtctaat gaagtagcaa
     2461 ggtatatagt agctaagtta aatggaaaaa ttgtcggata tgtgggaatt tggcttatac
     2521 ttgatgaagg gcatataaat aatgtggctg ttcatagtga ctatagaggg aaaaaaatag
     2581 gggacaagtt gataaaaggc atagttgatt tatgtaaaga taataatatt gcatctatga
     2641 cattagaagt tagagcatca aataaaatag cacaaaattt atatagaaaa tatggattta
     2701 aaatgggtgg aataagaaaa gaatactaca gtgataataa agaagatgca attataatgt
     2761 ggaaccaatt aaaagaggtg tagagtttat gagtgatata ataacattag ctatagagag
     2821 tagttgtgac gaaacagctg catcagtttt aaaaaatggt agagaagtac tttcaaacat
     2881 aatctctact caaatcgaga cacataaaaa atttggtggt gtagttccag aagtggcctc
     2941 aagaaaacat gtggaaaata ttgatatagt tgttcaagaa gctttagaca aagcaaatat
     3001 aggttttaat gatatagacc atatcgcagt tacatatgga cctgggctag taggtgcttt
     3061 attggttggt ctatcttatg ctaaagcact tgcatatact ttgaatattc cacttgtagg
     3121 agtaaatcat atagaagggc atctaagtgc aaactatata gagcataagg atttaaaacc
     3181 tccttttata acattaatag tatctggagg tcatactcac ttggttgaag taaaagatta
     3241 tggcaagtat gaaattttag gaaaaacaag agatgatgca tctggagaag catttgataa
     3301 gatttcaaga gctatgaatt taggctatcc aggaggtcct attattgata atctggccaa
     3361 gaatggaaat aaacatgcta tagaatttcc aagagcctat ttagaagaag atagctatga
     3421 ttttagtttt agtggattaa aatcttctgt attaaattat ctaaatggaa aaagaatgaa
     3481 aaatgaagaa atagtagttg aagatgtagc agcatctttc caagaggctg tagtagaggt
     3541 tttatctact aaagcactaa aagcagtaaa agacaaagga tacaatatca taactctttc
     3601 tggtggggtt gcttctaatt cggggcttag agctaaaata acagagcttg ctaaagacaa
     3661 tggtattact gttaagtatc ctcctctaat tttatgcaca gataatgcag ctatgatagg
     3721 gtgtgcagga tattataatt ttataaatgg gaaaacgcat gatatgagtt taaatgcagt
     3781 tccaaattta aagataaatc agtagagtta gatgaatttg taaatataaa agtattgttt
     3841 caaaaaagta agtagaagtg aaattaaata taataaaaat aaatatagta tatattaaaa
     3901 cttgaagtaa aactatttat aatctttata ttccaatgaa ttgcaacaaa ttttaggata
     3961 tacagattgt ctttggctta gatttgcttc aagtttattt ttttctatag aaagattatt
     4021 ttaaaaagtg ggtaatatac cctttttttt cattcttaaa aagttttata ctaattatag
     4081 aaagattaag gaggggaaga aatgagtcaa agtaaagaag acaaaataag aagtattttg
     4141 gaagcaaaaa atattaaatc aaattttcaa aataaagaaa atttaagtga atttaatgaa
     4201 aaaaaagcaa gtaaaagagc agaagactta ttagatgtat attataatac attatctact
     4261 gctgatatgg aatttccata ttggtataat agagaatata gaaaatcaga tggtgatata
     4321 ccagtagtta gaagagctaa agctcttaaa gctgcttttt cacatatgac accaaatata
     4381 atacctggtg agaaaatagt aatgcaaaag acacgccatt atagaggttc ttttccaatg
     4441 ccatgggtaa gtgaaagctt ctttgttgct caaggagaac aaatgagaga agaggcaaag
     4501 aaattagcca gtaacacagc tgatgaactt acaaaatttg ggtctggtgg aggaaatgtt
     4561 actgaaagtt ttggaaatgt cgtttcaata gctggtaaat tcggaatgag aaaagaagaa
     4621 gtacctgtac ttgtaaaaat ggcaaaggaa tgggttggaa agtctgttga agatttaggg
     4681 tttcactatg aaaaaatgat gccagattat gatttaaaag aaaacctaat gagtacactt
     4741 atatgcatgt ttgattcagg gtatacactt cctcaaggta gagaagttat aaattatttt
     4801 tatcctttaa attatggatt agatggaatt atagaaatgg caaaagaatg taaaaaggca
     4861 gttgcaggaa atgcatctgg agatgggctt attggtatgg accgtctata tttttatgag
     4921 gctgttattc aagttattga aggattacaa acttggattt taaattatgc aaaacatgca
     4981 aaatatttag aaagtataga gacagattta gaagctaaaa aagaatactc agatttagtg
     5041 gagatattag aacatattgc acataaacaa cctcgtacat ttagggaagc tcttcagttg
     5101 acttatacaa ttcatatagc atcagtaaat gaggatgcta tatctggtat gtctatagga
     5161 cgttttggtc aaattttata tccttggtat gaacaagata tagaaaaagg actaattaca
     5221 aaagaagagg ttatcgaatt attagagctt tatagaataa aaatcacttg tattgactgt
     5281 tttgcttcag cgggcgtaaa tggaggagta ctatcaggca atacatttaa tactttatct
     5341 ataggtggat taaaagaaga tggttctaca ggagctaatg aactagaaga gttattatta
     5401 gaagcaagta tgagatgtag aactcctcaa cctagtttaa ctatgctata tgatgaaaag
     5461 cttccagaag atttcttaat gaaagctgct gaatgtacaa agcttggttc tggttatcca
     5521 gcatgggtaa acaactcaaa tggaacaaca tttatgatga aacaatttgc tgatgaaggt
     5581 atgactgttg aagaagcaag agcatttgca ttaggtggat gtttggaaac gtctccaggg
     5641 tgttggaaac agttaacttt aaacggaaaa acatactcta ttgcaggtgg agcaggtcaa
     5701 tctgctggct ctggagttca ctttatagct aatccaaaaa ttttagaact tgttcttatg
     5761 aatggtaaag actatcgtat gaatatacaa gtatttgagc ctcataataa gccattagat
     5821 acttatgaag aagtcataga agtatttaaa gattattata aacaagcaat aaatgtttta
     5881 gaaagagcaa ataacattga gttagatata tggagaaaat ttgatacttc tataataaat
     5941 tcactactaa agccagactg tttagacaaa ggtcaacata taggaaatat gggatatcgt
     6001 tataatgcaa cattaaacgt agaaacttgt ggtactgtta ctatggtcaa ttcatttgca
     6061 gctcttaaga aacttgtata tgatgacaaa gcatttacaa tagaggaaat gaaggatgcc
     6121 atattaaata attttggatt taaagatgca ttagaagtag gaaattattc tatggcagac
     6181 caagtaaaag tagataagac tggtaaatat gatgctattt ataaggcctg tcttgatgca
     6241 cctaaatatg gaaataatga tttgtatgca gataatattc ttaaaaacta tgaggtatgg
     6301 ttatcaaaag tatgtgaaga agcacaatct ttatatgcta agaagatgta cccttgccaa
     6361 atatctgttt ctacacatgg gccacaggga gctgctacct tggctactcc agatggtaga
     6421 ctaagtggaa caacatattc agatggttca gtctctgcat atgcaggaac agataaaaat
     6481 ggagtatatg cattatttga atctgcaact atttgggacc aagcagtagt tcaaaattct
     6541 cagatgaatt taaaacttca tccaactact ataaaaggac aacaaggaac taaaaaatta
     6601 ttagatttaa caagaagtta tttaagaaaa ggtgggttcc atattcaata taatgttgta
     6661 gattcagaaa cattaaaaga tgcacaaaaa aatcctgaca attatcgtca attaatggta
     6721 cgtgttgctg ggtttactca atattggtgt gaattaggta aaccaataca agatgaagta
     6781 attgctagaa cagagtatga aggggtgtaa aatatgagaa agcatagtga ttgtatgaat
     6841 ttttgtgcag tagatgcaac caaaggaatt tgtagattat caaaacaaat gattaattta
     6901 gatgatgcag catgtccaga gataaaagta atgccaaaat gtaaaaattg taaaaatttt
     6961 gttgaagcta atgatgaagg tataggcaaa tgtgttggcc tagagaaaga agactgggta
     7021 tattcaacat tgaatgcaat tacttgtgaa ggacatgtgt ttaatgagta gtcaaaagca
     7081 attagaaggc atgatttttg atgtacaaag cttttcagtt catgatggtc caggttgtag
     7141 aacaactgta tttttaaatg gatgtccact gagttgtaaa tggtgtgcaa atccagaaag
     7201 ttggactgta agaccacata tgatgtttag tgaattatct tgtcaatatg aaaatggatg
     7261 tactgtatgt catggtaagt gtaaaaatgg tgcattgagc tttaatcttg ataataagcc
     7321 agttatagat tggaacatct gtaaggactg tgaaagtttt gaatgcgtca actcatgtta
     7381 ttataatgcg tttaaattat gtgcaaaacc ttacactgta gatgaacttg tgcaggtaat
     7441 taaacgtgat tctaacaatt ggagaagtaa tggaggagta acatttagtg gaggtgagcc
     7501 actacttcag catgaatttt tacatgaagt attattaaag tgtcatgagg taaatataca
     7561 cactgccatt gaaacgagtg catgtgtatc aaatgaagtt tttaataaaa tatttaacga
     7621 tattgatttt gcatttattg atataaagca tatggataga gaaaaacaca aagaacaaac
     7681 aggtgtgtat aatgatttaa tattagaaaa tatatcaaac cttgcaaatt ctgattggaa
     7741 tggaagatta gtacttagag taccagttat ctctgggttt aatgatagtg atgaaaatat
     7801 aagtgatatt atatctttta tgcacaaaaa taatttggta gaaattaatc tattaccatt
     7861 tcacagatta ggagaatcaa aatggactca attaggcaag gaatatgagt attctgataa
     7921 gggtgatgtt gatgaaggtc atcttgaaga attacaagat atatttttgg ataatggtat
     7981 agcttgttat gttggtcatg agacagcttt ctaaaattaa atacaagttt taattaaaaa
     8041 aggatgttac actaaaagct agagttacat ccttttttat gtggttttta tgtagaaata
     8101 attaagcaaa gttttatatg ttttatatat tgttcatatt actttatgtc cataaatatt
     8161 gctttaaata tactttatat attttttata gtttaaatta cagttagaga atatattacg
     8221 tttacttcat tgaagcagaa gttttatggt aaaatactac tatgaaaaaa tttaatagaa
     8281 tatattttag aaacaactaa aaataatggt taggaggaag acatctatat gaacaaatca
     8341 tggagcaact tgattattaa gaataaagac agaaaagtac gttcagggtg gattattata
     8401 gctgttatgg ctacctttta catacttcag tattatttaa gcatgatact tgtggaaata
     8461 ataagaaaaa tcctggttgc tacaggtgac attaatttaa aaacaaacta tttttcagaa
     8521 tatgctaatt ggttaaatga tgttgggtta cagattgtat ttcaaattct aacagattca
     8581 cttatgatta ttattcctat aattgtatgg agatttatta tgaaaaatcc tattagtgaa
     8641 atgggattac attcttatag aattaagaaa aaagaaggat atgtaggaat gttattagga
     8701 atatttaatt gtacagtaat ctttattata acaataacaa ttggtggtgg tcatgttact
     8761 tcatgggtac caaaaatttc acctttaaca gtttcttgga tactggtctt tattttagtt
     8821 gcttttgcag aagaaatttt aaatcgtggt ttttttatgg cagttttacg aagatgtaaa
     8881 aatatttatt ttataatgat tgttccatct gttatttttg ggttgattca tatatggaat
     8941 cctgatgtta cttttttgtc tgtaattaat attataatta ttgggattct tttttcatat
     9001 atgtttataa aatcgtcgaa tatatggatg tgtataggtt atcattttgc atggaatgta
     9061 tttcagggga ttatatatgg tatgcctgta agtggacttc aagttccagg gattataact
     9121 acacaaatca ctcatggtaa tctattaaat ggaggaatgt ttggtattga aggtggaatt
     9181 ttgactactt ttgttaatct acttagcttt atatttgtat ggtattacta tagagattct
     9241 aagtatgatt ttgttagtga taaagtaaat gtgaccaata tcaagaatta gttgattata
     9301 ataaatttta ataatatctt ctaagcttaa agtattctgt aggattgttg gaaatttgca
     9361 aaaatataaa aataacttgg atattctatt tactccaagt tatttttata ttcttttgta
     9421 aatagaaatg gaacataaaa aattaaaaca accatagata ctattagtat atcatggtaa
     9481 gtgatagttg attctttaga atttgtgata agaaaagcac tattatttaa aacatgaatt
     9541 atagcacaaa accagatatt cttagttttt atataaagat atcctaaaaa tattgaaatt
     9601 ccaactatat gtattgaacg taatattatt cctaatattg gcgcttcagg tgtatataat
     9661 gtaaatatta gtggtaagtg ccacaagctc caacacatac ctaatattat aactcctaac
     9721 tttttaccaa atttatcaaa aaaaatattc tgtaaaaaac ctctccaggc atattcttca
     9781 ctaaaaaaat ataaatagta gttaatgatg ttattggagt aaatataaat tttatgatac
     9841 cactataatt aggttgagcc ccttgtagta ttagtataaa tgatgttgga acaattacag
     9901 aaatcataaa gtaaataatt aataaagaac ctagtttaat attttttaca ggatataaat
     9961 tttttttatt tgatgaatat attataattg ataagctact tat
//
LOCUS       DBGJFF010000138         9785 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.140, whole genome shotgun sequence.
ACCESSION   DBGJFF010000138 DBGJFF010000000
VERSION     DBGJFF010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9785
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.140"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     misc_binding    158..369
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            532..1821
                     /locus_tag="ACOIAF_003243"
     CDS             532..1821
                     /locus_tag="ACOIAF_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HGZ2670238.1"
                     /translation="MEEEKINYALEQIPENQKRGWVAMFSVLVAIGVDLSSVILGAEL
                     AQSMPMKQAILSVIVGSFFSAILYTTCSLVGSSTSLSTSMITKYVFGEAGAKIFSLVI
                     GVSLLGWFGVQVGFFAQNAQIIIKDIFNLDVSMQILSLIGGLLMMSTAIYGYKAMEKL
                     SVYSVPFLLVLMMLTIFLAFRANGISVDDNMKSTMTFAGGVSLSMSIIIVGAIVSPDI
                     SRWAKSRRDCALSSFLGIQFGNAFMIIVSIVLVKCMGTSDIMRIFITLGIAIPGIIVL
                     TLAQWTTNTSNVYSASLSIALVLKKAPEKVLTIVLGIIATLLAVFGIYEGFIGFLNLL
                     GIVIAPVGGVYTAEYYIVKQELKGFDKGVLYKPIVKRSIVSWIIGILITYLSTYGFIT
                     LTTIAPLDGFIAGFVVQSIIGKVLCSTKNKQEIDKAV"
     gene            1875..2954
                     /locus_tag="ACOIAF_003244"
     CDS             1875..2954
                     /locus_tag="ACOIAF_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF917 domain-containing protein"
                     /protein_id="HGZ2670239.1"
                     /translation="MRFLTEESVDKIAVGAAVLGTGGGGDPYVGKLVAKQAIKKYGPV
                     KVISLDELDDDALVVPVSGMGSPVITIEKLLSEVELTTPLEIMEKLLNRKVDVIIPIE
                     IGGINSLMPIAVAAKKGLPILDADSMGRAFPEAQMVTFYLEGYEASPAVMSDEKGNSA
                     ILYPVDGIWSERLARTLTVEMGGSSSISDYNLSGAQVKKAAIGNTLTIAETIGGFLLE
                     NKSDSKGAVENILKELRGYKLFEGKVIDIKRELKGGFTRGHAFFAGINEYDGEYSILF
                     QNENLIAKKGDTPLCITPDLIAVLDLETGFPITTERIKYGSRVMVVAFPCNEKWRTEK
                     GIETVGPGYFGYDVEYKTVEELQGK"
     gene            2975..4519
                     /locus_tag="ACOIAF_003245"
     CDS             2975..4519
                     /locus_tag="ACOIAF_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase N-terminal
                     domain-containing protein"
                     /protein_id="HGZ2670240.1"
                     /translation="MYRVGIDVGGTNTDACILDGELKVIHSVKVATTKDVETGVYNAL
                     KKVIDESKIDHSLIKYAMLGTTHCTNAIVERKRLNNVAMIRIGKPATTAILPFIDWPE
                     DLREKIELDSILVSGGYEFDGRKINELCKDEVVEFCNRIKGKVESVAISGVFAPVNKQ
                     QEEEVAKWVREILGDIPISLSNEIGNIGILERENGAILNSALSQVGKAVSLGFEKALK
                     DLGIDAQIYFSQNDGTLMNLEYTMKYPIFTIGCGITNSIRGASFLSKVKNAIVLDVGG
                     TTSDLGVLYNGFPRESSIPVTIGGVHTNFRMPDVLTIGLAGGTCVKGEKDNIKIGPES
                     VGYRITEDAIIFGGDTLTLSDVVTKLDMAFEDRKSNVENLDKEYCNEVYKKVILKLED
                     AVDQMKTSQDDVELVLTGGGSIIVPEKLKGISNVIRPPHYTAANAIGAALGQISGDVE
                     KVYSLDKMSREDAIEDAKKEAVNKALKAGAKEDTIEVLNIEDVPLAYLPGNALLIKVK
                     VVGDLI"
     gene            complement(4682..5224)
                     /locus_tag="ACOIAF_003246"
     CDS             complement(4682..5224)
                     /locus_tag="ACOIAF_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HGZ2670241.1"
                     /translation="MKSYIALLRGINISGKNKIIMSELKASFVELGYSEVSTYLNSGN
                     VVFLSDVDDSKVISNTIRLMIKKQFELEIPVFIISQEELKDILNNAPNWWGDNNKEIY
                     DNLIFMFPNLSYDEFYDEVGNPKEEYEKVYHYKNAIFWSFSRKDYKKTNWWSKTASSN
                     VSDKITIRTANTVRKIVGMR"
     gene            complement(5372..5656)
                     /locus_tag="ACOIAF_003247"
     CDS             complement(5372..5656)
                     /locus_tag="ACOIAF_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGZ2670242.1"
                     /translation="MAKNTKQTTVHIDETLLKEVKRIGINENKSISQIINESIIDYIL
                     VYRENEDKEKNEHDKNLNETINEFKKEEEKRMQAKSAARAIAEAKKRVLY"
     gene            complement(5719..6483)
                     /locus_tag="ACOIAF_003248"
     CDS             complement(5719..6483)
                     /locus_tag="ACOIAF_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HGZ2670243.1"
                     /translation="MNNYLKFATYQLIGFTSSIPRLIKIKKNPDKFSLKEKFEFMQKQ
                     AKKSLDIVNIELNIIGKETLPKEPLLFVVNHSSMLDSFILTASVERPIGCVIADEPVW
                     RNIPIFKEWAKLLRCVYVNRKNNREGIKSIAQASQNILTGQSMAVFPEGDLTWIKEPN
                     SLVSEFRSGALKIAYKAKCPIVPLVIKNSKDTYEGYQPIGKINSVPVEVEFLEPIYDH
                     IENPRLKSSVLGENIKNKMINTIENFRKSNKTFKEF"
     gene            complement(6765..7031)
                     /locus_tag="ACOIAF_003249"
     CDS             complement(6765..7031)
                     /locus_tag="ACOIAF_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HGZ2670244.1"
                     /translation="MIESRCGILCYECGYKEKVNCKGCTEIEKPFWGEQCPVKSCCED
                     KNLTHCGLCDTFPCEMLNQFAYDKEQGDDGKRISQCKKWAVLAL"
     gene            complement(7073..7882)
                     /locus_tag="ACOIAF_003250"
     CDS             complement(7073..7882)
                     /locus_tag="ACOIAF_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGZ2670245.1"
                     /translation="MNKIINNILNTCKQYTYILPHKLLRQYIAHYTISIPDTSIKENL
                     TLIPDASGCMIFKFDKKGIESAFWGATTKTTIVKNDIENVLFRVFVEFRPGGVYYLTG
                     LSQRESTDLKISLEDFNTLFSLEVNSIFERTSTIKELVEQLDMLFLSYLLKSNIVDMT
                     IPILENARKQNSIMSVKNISQISCYSERHLNRIFNNSLGMSVKSYLRLLRINLVLQEI
                     QNNKIPFATLAQDIGYYDQSHFINDFKSICGVNPTTYIKNLSDFYNEKYKF"
     gene            8195..8476
                     /locus_tag="ACOIAF_003251"
     CDS             8195..8476
                     /locus_tag="ACOIAF_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670246.1"
                     /translation="MASKQTPRNNLEKFLRIRVLSHTGDKIHIKLPVNFAKRMIENNA
                     LDLFNGKDDVVDGKKVTEILVKAFDYDLTGEVVHLERKNGDIIKVSIVQ"
     gene            8740..8994
                     /locus_tag="ACOIAF_003252"
     CDS             8740..8994
                     /locus_tag="ACOIAF_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670247.1"
                     /translation="MENKDFKLTICIFAFVIGVVVLFCCIGLGEFSAEHIIQQNGGIM
                     DTDQYNIYLDQSISQYRNFGSILALLGGIGILFDKYIRKY"
     gene            9039..9362
                     /locus_tag="ACOIAF_003253"
     CDS             9039..9362
                     /locus_tag="ACOIAF_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6054 family protein"
                     /protein_id="HGZ2670248.1"
                     /translation="MATEIFTVKITPLEAQKMIKENLDADLVFSDSYNLGDDKFILIT
                     TFEKYYARNNSDAGLIVVYENTTGKTVIKATSTGSAIGILKIDWGAGGNLLKRVKNIL
                     ADYII"
BASE COUNT         3568 a         1129 c         1567 g         3521 t
ORIGIN      
        1 attttgatga aatttaaaaa aatacattat ataaatatag taatagtatg aaaatttaca
       61 tataaaacta aaaaatgcaa gtaattgact ttttaagttg acaaaatatt gcatatatat
      121 tatgatataa atcataaatt aactacaaat caatattata tattcgtaga agaagaaagt
      181 agcataaagg aattttacag agagttagat ttggctgaga actaatattg gaatttatgt
      241 gaaaaggact ttggagaata ctattgtaat tatgttagaa tagtggttta attcgcctta
      301 agaaaatgag tgagtaaaat taatttttac taataagagt ggtaacacgg atttatattc
      361 gtctcttgga tttttccaag aggcgttttt tattataaac ttatttcaga aaatttaaaa
      421 ctatatatta aatgaaaatt ataaaaatat gatagttaat aaatttataa attagaaaat
      481 tgtaaaatat gctaaggtca gtagttttac gatttaaatg gagggatgaa aatggaagaa
      541 gaaaaaatta actatgctct ggaacagatt ccagagaatc aaaaacgtgg ttgggtagct
      601 atgttttcag ttttagtagc aataggtgtg gatttatcat ctgttatttt aggcgctgag
      661 ttggcacaga gtatgccaat gaaacaagca atattgtcag taattgttgg ttcatttttt
      721 tctgcaattt tgtatactac atgctctttg gttggctcat ctacaagttt atctacatct
      781 atgataacaa agtatgtatt tggagaagct ggagcaaaaa tattttctct agttataggg
      841 gtttctctat taggttggtt tggagttcaa gtaggtttct ttgctcaaaa tgcacagata
      901 ataataaaag atatatttaa tttagatgta agtatgcaga ttttaagcct aataggtggt
      961 ctcttgatga tgagtactgc aatttatgga tataaggcaa tggagaagtt gagtgtgtat
     1021 tcagtaccat ttttattagt tcttatgatg ctaactatat tcttagcttt tagagcaaat
     1081 ggaatttcag ttgatgataa tatgaaatct actatgacat ttgcaggtgg agtttctctg
     1141 tcaatgagta taataatagt tggtgcaatt gtatctcctg atataagtcg ttgggctaaa
     1201 agtagaagag actgtgctct ttcaagcttt ttgggaatac aatttggaaa tgcttttatg
     1261 ataatagttt cgatagtatt agtaaaatgt atgggaactt cagatattat gagaattttt
     1321 attacattag gaattgctat accaggtata atagtactta cattagcaca atggactaca
     1381 aatacaagta atgtatattc agcatcatta agtatagctc ttgtacttaa gaaggctcct
     1441 gagaaagtat taactatagt ccttggtatt atagcaacat tattggctgt atttggaata
     1501 tatgaaggtt ttataggatt tttaaattta cttggaattg taattgcacc tgttggtggt
     1561 gtatatacag cagaatatta catagtaaag caagaactta aaggctttga taaaggagta
     1621 ttatataaac caatagttaa acgttcaata gtctcttgga taattggtat attaataact
     1681 tacctttcaa catatggatt tataactctt actactatag cacctttaga tggttttata
     1741 gcagggtttg tagtacaatc tattattgga aaagtgcttt gttcaacaaa aaataagcaa
     1801 gaaatagaca aagcagtata aagaaatttg tttataaagt ataaaataaa ttagaaatat
     1861 gaggagaaag taacatgaga tttttaacag aagaaagtgt agataaaata gcagttggtg
     1921 cagcagtgct tggaactgga ggtggaggtg acccatatgt aggtaaattg gttgcaaaac
     1981 aagccattaa aaagtatggt cctgtaaaag taataagttt agatgaactt gatgatgatg
     2041 ctttggtagt tccagtatct ggtatgggtt ctcctgtaat tacaatagaa aaattacttt
     2101 cagaagtaga gttaactacc cctttagaaa taatggagaa attattaaat agaaaagtgg
     2161 atgttataat tcctatagaa ataggaggga taaactcact tatgcctata gcagttgctg
     2221 ctaaaaaagg acttcccatc ttagatgcag attctatggg tagagctttc cctgaagctc
     2281 aaatggtaac tttttatctt gaaggatatg aggcatctcc tgccgtaatg tctgatgaaa
     2341 aaggtaattc tgccatcctt tatcctgtgg atggaatttg gtcagaaaga ttagctagaa
     2401 ctttaactgt agaaatggga ggaagttctt ctattagtga ctataattta agtggagctc
     2461 aagttaaaaa agctgctata ggtaatactc ttacaatagc tgaaactata ggagggtttt
     2521 tacttgaaaa taaatctgat tcaaaaggtg ctgttgagaa tatactaaaa gaattgcgtg
     2581 gatacaagtt atttgaagga aaagtaattg atattaagag agagttgaaa ggtggattta
     2641 ctagaggaca tgcctttttt gctgggatta atgagtatga tggagagtat tctatattat
     2701 tccaaaatga gaatttaatt gccaaaaaag gtgatacacc actttgtatt acaccagatt
     2761 taatagcagt attagattta gaaactggat ttccaataac tacggagaga ataaaatatg
     2821 gctctcgtgt tatggtagta gcttttcctt gtaatgaaaa gtggagaaca gaaaaaggaa
     2881 ttgagacagt tggacctgga tactttggat atgatgtgga atataaaaca gtagaagaat
     2941 tacaaggaaa ataaaagttt aggaggaaat tttgatgtat cgtgtaggaa ttgacgttgg
     3001 aggaacaaat acagatgctt gtatactgga tggggaatta aaagtaattc attcagttaa
     3061 ggtggcaact acaaaagatg ttgaaactgg tgtttacaat gccttaaaaa aagttataga
     3121 tgaaagtaag attgaccatt cattaataaa atatgctatg cttggtacaa ctcattgtac
     3181 aaatgctatt gtagaaagaa aaagacttaa taatgtggct atgataagaa taggaaagcc
     3241 ggccactact gccattcttc catttataga ttggccagaa gacttaagag aaaaaataga
     3301 attagattca atattggtat ctggtggata tgagtttgat ggcagaaaaa taaatgaact
     3361 ttgtaaagat gaagttgtag agttttgcaa tagaataaaa ggcaaagtgg aaagtgttgc
     3421 aataagtgga gtatttgctc cagtaaataa acaacaagaa gaagaagttg caaaatgggt
     3481 tagagaaatt ttaggggata ttcctatatc tttatcaaat gaaataggta atataggtat
     3541 attagaaaga gaaaatggag ctattttaaa ttctgcattg tctcaagtag gtaaagctgt
     3601 atctttagga tttgaaaaag cacttaaaga cttaggtata gatgctcaaa tatatttcag
     3661 tcaaaatgat ggaacattaa tgaatttaga gtatacaatg aaatacccaa tttttacaat
     3721 tggatgtgga ataacaaatt ctataagagg agcttccttc ttatcaaaag taaaaaatgc
     3781 aatagtatta gatgttggtg gaactacaag cgacttgggt gtactatata atggattccc
     3841 tagagaatca tctattccag ttacaattgg aggagtacat acaaacttta gaatgccaga
     3901 tgttttaact attggccttg ctggtggtac atgtgtaaaa ggcgaaaaag ataatataaa
     3961 gataggtcct gaaagtgtgg gatatagaat aactgaagat gcaataatat ttggtggaga
     4021 cactctaact ttatcagatg ttgttacaaa attagatatg gcgtttgaag atagaaaaag
     4081 caatgttgaa aacttagata aagagtattg taatgaggta tataagaagg ttattttaaa
     4141 attagaagat gctgtagacc aaatgaagac tagtcaagat gatgttgaat tggttttaac
     4201 tggtggagga agtataattg ttcctgaaaa actaaaaggc ataagcaatg ttataagacc
     4261 tcctcattat actgctgcaa atgctattgg tgcagcttta ggacaaataa gtggagatgt
     4321 agaaaaagta tacagtttag ataaaatgtc gagagaagat gccatagaag atgcaaaaaa
     4381 agaggctgta aataaagctc taaaagctgg tgctaaagaa gatactatag aagtattaaa
     4441 tatagaagat gttccacttg cataccttcc tggtaatgca cttcttataa aagtaaaagt
     4501 tgttggtgat ttaatttaaa gaataaatat aagtatttcc agaattatat aaatatttct
     4561 ggactcattt ttaaacttat tttatcaata aaaacagcat aacttttgac tatattctta
     4621 taagtttcca aacagctgat atatacctat tttttgaggt ttatcggctg tttgttatct
     4681 tttatcgcat tccaactatt tttcttaccg tatttgctgt acgaattgta atcttatcac
     4741 ttacatttga actggcagtc tttgaccacc aatttgtctt tttataatct ttacgactaa
     4801 aagaccaaaa gatagcattt ttataatgat atactttttc atactcttct ttaggatttc
     4861 caacctcatc ataaaactcg tcataagata agttaggaaa cataaaaata agattatcat
     4921 atatttcttt gttgttatct ccccaccaat taggagcatt atttagtata tcttttaact
     4981 cctcttgtga aataataaaa actggtattt ccaattcaaa ctgttttttt atcattaatc
     5041 ttattgtatt tgagataacc ttgctgtcat ctacatcact taaaaagaca acattaccac
     5101 tattaaggta tgtagaaact tctgaataac caagttctac aaagcttgct tttaattcag
     5161 acataattat cttgttctta ccacttatat tgatacctct taataatgcg atataacttt
     5221 tcatataata atttcacctc taaacattta catttttcta tgtagtacaa acttgaattt
     5281 gcatctttta taattctttc aaaacttctt catatgtaat ttttttaata taaaaaaatc
     5341 ggtgcttaat ttgtaccgat ttttgttttt cttagtaaag aactcgtttt tttgcttctg
     5401 ctatagctct agcagcactt tttgcttgca ttcttttttc ttcttctttt ttaaattcat
     5461 ttattgtttc atttagattc ttatcatgtt catttttttc tttatcttcg ttttctctgt
     5521 atactaatat ataatctatt attgattcgt ttattatctg agatatactt ttattttcat
     5581 taattcctat tctcttaact tccttaagta gagtctcatc aatatgaacc gttgtttgct
     5641 ttgtattttt tgccacctta atcacctctt aaaaaaaatt ctaacattaa aatttttttt
     5701 agtcaataaa ttttaatatt aaaattcttt aaatgtttta tttgattttc taaaattttc
     5761 tattgtattt atcatcttat tttttatatt ttcacctagt acagaagact ttagtctagg
     5821 attttcgata tggtcataaa ttggttctaa aaattcgact tcgacaggaa cagagttaat
     5881 tttaccaata ggttggtatc cttcataagt atcttttgaa ttttttatta ctagtggaac
     5941 aatagggcat tttgctttat atgctatctt taaagctcca cttctaaact ccgaaaccaa
     6001 tgaatttggt tctttaatcc atgttaaatc tccttctgga aacactgcca tactttgacc
     6061 tgtaagtata ttttgagagg cttgggctat actctttata ccttcacgat tgttttttct
     6121 gtttacatat acacatctta gtaattttgc ccattcttta aatataggta tatttctcca
     6181 aactggttca tctgcaatta cacatccaat tggtctttct acacttgctg ttagtatgaa
     6241 gctgtcaagc atactagaat gatttacaac aaatagaagt ggttcttttg gcagtgtttc
     6301 cttaccaatt atattaagct ctatgttcac tatatctaaa gattttttag cttgtttttg
     6361 cataaattca aatttttcct ttaagctaaa tttatcagga ttctttttaa ttttaataag
     6421 cctaggtata gaagatgtaa aacctataag ttgatatgta gcaaatttta agtaattatt
     6481 cataaaatta tctccttaaa ataaaattat ttattactgt aaagatttta acataaaata
     6541 aaatttaagt ctattttaat tcaattttgt aacatatatt ttaaatagac agagattgag
     6601 attttatatt tccaatttta gtaatcagat tttatcaata aattactatt ctattaattt
     6661 aattaaataa cctttaaatt taattataaa aactgttgca gaaatttcta caacagttta
     6721 aaaaatttac cttacaaaaa cttattttat aacaagagag tatattataa ggctagtaca
     6781 gcccattttt tgcactgaga aattcttttg ccatcatcac cttgttcttt gtcatatgca
     6841 aattgattaa gcatttcaca tggaaatgta tcacatagac cacaatgtgt aaggttttta
     6901 tcttcacaac aagattttac aggacattgt tcaccccaaa atggtttctc aatctctgta
     6961 caacctttac aattgacttt ttccttgtat ccacattcat aacataaaat tccacatctt
     7021 gattctatca ttttaagtca ctccttaaat ataaatttta tatgattgta tatcaaaatt
     7081 tatacttttc attgtaaaaa tcggacaaat tttttatata ggttgttgga ttaacaccac
     7141 aaatagattt aaaatcgttt ataaaatgag attggtcgta ataaccaata tcttgtgcta
     7201 aagttgcaaa gggtatttta ttattttgta tttcttgtaa tacaaggttt atacgaagta
     7261 atctaaggta tgatttaaca ctcataccaa gagaattatt aaaaattcta tttaaatgtc
     7321 gttcactata acaactaatt tgtgaaatgt ttttaacaga cataatagag ttctgttttc
     7381 tagcattttc aagaattggt atagtcatat ctactatatt tgatttcaat aagtatgata
     7441 agaataacat atctaattgt tctactaatt cctttatagt acttgttctt tcaaaaatgg
     7501 aattaacctc taagctaaat aatgtgttaa aatcttcaag tgaaattttt aaatctgtag
     7561 attctctttg agaaagacct gtcaaataat atactccacc tgggcgaaac tcaacgaata
     7621 ctctaaaaag tacattttct atatcatttt ttacaatagt agtttttgtt gtggctcccc
     7681 agaatgcgct ttctatacct tttttgtcaa atttaaatat catacatcca ctagcatcag
     7741 gtattaatgt aagattttct ttaatacttg tatctggaat agatattgta tagtgtgcta
     7801 tatactgtct taacaattta tgtggcaaaa tatatgtata ctgtttgcag gtatttaaaa
     7861 tgttattgat tattttattc ataagtatat cctcctgata tataaaaaat aagtatacta
     7921 atttattttt tatataataa accagttttg ttttaattga aatattctaa taagatattt
     7981 tgttaaattt agatacaata attgtaaagt aaaatctcaa aatatacaat tattataaat
     8041 catagatatt tattaaataa atatattaaa gaagaaggaa tataaaacaa gattagaaaa
     8101 taatagtgtt actttcataa taaatacata taaagatatt tttaagaata aaatttatta
     8161 ttaaataaaa aataaaggag agtgatttaa tagcatggca tcaaaacaaa caccaagaaa
     8221 caatttagaa aagtttttaa gaataagagt attatcacat acaggcgata aaatacatat
     8281 aaagttacct gtaaattttg caaagagaat gattgagaat aatgccttgg atttatttaa
     8341 tggaaaagat gatgtagtag atggtaaaaa agttactgaa atattagtta aggcatttga
     8401 ctatgattta actggagaag tagttcactt ggagagaaaa aatggagaca ttattaaagt
     8461 ttcaattgta caatagaatt attttatatg aattttattt ttgaatagtt ataaataatt
     8521 agaaatttgt aaaaaagtat aatatattat tagtaatggt acagaataca catttataat
     8581 ttatagatgt gtattttttg tttaaaaaat ttaaatatat aaaactataa aggttacaat
     8641 tttggaaata gatgtaaata tatgttgtat tgtataataa gagtaatata aaatctagta
     8701 aaaataatca aataaattta gcttatgaga gggataacta tggaaaataa ggattttaaa
     8761 ttaacaatat gtatatttgc ttttgtaatt ggagtagtag tattattctg ttgtataggt
     8821 ttaggtgaat ttagtgcaga gcatattatt caacaaaatg gtggaatcat ggatactgac
     8881 caatataata tttatctaga ccaatctatt tctcaatata gaaattttgg ttctatatta
     8941 gctctgttag gaggaatagg gatattgttt gataaatata tcagaaaata ctagagatta
     9001 tgttttgtct aaaataaatt cttatgagga gataaaaaat ggcaacagaa atatttactg
     9061 taaaaattac acccttagag gcacaaaaaa tgataaaaga aaatcttgat gcagatttag
     9121 tttttagtga ttcttataat ttaggagatg ataaatttat actaattaca acttttgaga
     9181 agtattatgc aagaaataat agtgatgcag gattaatagt agtttatgaa aataccacag
     9241 gaaaaacagt tattaaagct acttcaacag gcagtgcaat tggaatttta aaaattgatt
     9301 ggggtgcagg tggaaattta cttaaaagag taaaaaacat acttgctgat tatattattt
     9361 aattatttta tctaactgat aatatgtcaa gaaaatctcg taaaagaggt taagaacatt
     9421 tttataatat atagataaat aagaatttga aggtaaagtt tttaaggtat aaagaaaaat
     9481 aatataataa aacgaattgg ctttataatt ctttataagg caaagagcac atttctttat
     9541 ataattgttg gatattaaat aatatgtaaa tacaccattt acatataaaa gtttaattaa
     9601 tatgttaaga gaaaatgaaa tggaaattac taaaaaatat ttttaaattg gataatggtt
     9661 gatattatgt ttatacaaaa atcatgtaga attttatata ttttaaatat gatagtttaa
     9721 aattatgaaa gaatgataaa ttccagtttg tagggtaatt ttgataaata atattaataa
     9781 gtatg
//
LOCUS       DBGJFF010000139         9771 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.141, whole genome shotgun sequence.
ACCESSION   DBGJFF010000139 DBGJFF010000000
VERSION     DBGJFF010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9771
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.141"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            152..1582
                     /locus_tag="ACOIAF_003254"
     CDS             152..1582
                     /locus_tag="ACOIAF_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGZ2670249.1"
                     /translation="MSSQESKPKGNLISNFRDDMRDPKKRAIYTYSSSINLAYNFIGY
                     FVSTVTNIFLTDVAKLGVIMAGGMQIIQSFIKIAVAPITGTVFDKQPFKKGKYYPWLR
                     YAPAALALTYGIFFIVPLMKISPALLLPLMTALLLIASCAQQVVFTIIYAIYPVVAKT
                     PRDRVIGSTTTNIFKEVGKFLVGFSYPLLLVLFADVLGGEGMGYLGTYLFFAAGCLLV
                     FWFSSTEIIKSGAEEEVLQKEEKPKIKASEMAKALFTNVPLMMAFCLEFLICIRSIGL
                     GPLAPYYFKYVIEDERGLAVFLSIMPLVSVAFMFFAPVFIKICREQKLASIISFSICA
                     LCHLLVAWTPWGKTTLGVTMLLAIGGGFSNVVSIINLNFFAGSCDYGHWKSGKDLPGL
                     SMSLYPVAIQVGVLLATTIRTVLMNSMGYQADMVVTEAVKSGFINMISYSMAIPLIIA
                     VVIAILYPVSDKKLNQIREELNQRNA"
     gene            1685..2788
                     /gene="hisC"
                     /locus_tag="ACOIAF_003255"
     CDS             1685..2788
                     /gene="hisC"
                     /locus_tag="ACOIAF_003255"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HGZ2670250.1"
                     /translation="MIEKLLRNTVKELHQYVPGEPIEKVKEKYGVKEIIKLASNENPL
                     GPSPKAIEAMIEMLKQGQLYPEPEANELRRKLAEKLDLKPENFIVANGADNVITLIGE
                     AFINRGDEVIYCNPTFPSYRTATIKNEGIPVEVPLTEDYKYDLQGILDKITDKTKLIC
                     VCNPNNPTGTIVDDKELEEFLKKVPENIITILDEAYIEFLTVPNYVDGLKYVRENYNV
                     IVTRTFSKIYGLAGLRVGYGIAKDEIIRTLFTVKEPFSANRVAISGATAALDDEEFIK
                     ETYELNRVGMAYFKEEFTKMGFDVVDSQSNFLYVDMKTDIPKLFEDLKKRGFVIRPSA
                     THARVSIGTMEENRKFVAVLKEILNIKGEPSIG"
     gene            2821..3039
                     /locus_tag="ACOIAF_003256"
     CDS             2821..3039
                     /locus_tag="ACOIAF_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HGZ2670251.1"
                     /translation="MNLKINAERCKSCEYCVISCKKGALKISSKINKEGYAHVEVDES
                     KCILCGICYQVCPDNVFEIIKEEACQEA"
     gene            3056..4105
                     /gene="vorB"
                     /locus_tag="ACOIAF_003257"
     CDS             3056..4105
                     /gene="vorB"
                     /locus_tag="ACOIAF_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HGZ2670252.1"
                     /translation="MKKMWKGNHAIAEAALRGGCEFYAGYPITPQTEVMEFLSHRMSE
                     LGRTFIQSENEMAAIYMVYGAYASGMRSMTSSSGPGISLKQEGISYLCANHYPCVIVN
                     VQRWGPGLGSLDSAQTDYLRDTRGGGNGDYHLIVYAPNSIQETVDLMYNSFDVAEKYR
                     VPVEILTEAALGQMMEPVEFPEFKKREGDLGWTYDGSNRDHAKVADNQKPTFCMEKRM
                     RITENEQQWEDYQVEDAEYVFVAFGIPSRTTMNAVRRLREQGEKVGIIRPITVWPFPY
                     KAFEKVSKDVKGFISVESTDTGQLVEDVALASKKVCKENVPVYGLFSGNHIPKTLQVM
                     DTYSKIKSGEIKEVF"
     gene            4107..4847
                     /locus_tag="ACOIAF_003258"
     CDS             4107..4847
                     /locus_tag="ACOIAF_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HGZ2670253.1"
                     /translation="MATEKLTPSVVSIPNKFCPGCGHGIVNRIIAEVIEEHGYEKNHV
                     LTLGVGCVCNMNFSWNGDKMQTAHGRASSTAIGVKVALPDTLVMTYQGDGDAYVIGLS
                     ETLNTAYRNHNVTVFVINNNNFAMTGGQMSWTTMPGQVTTTSVNGRDIKTTGSPLKVP
                     EMVANFFDVAYVARGSVHSPKEIINLKKYIKNAIEAQLNGEGYSLVEILAPCPTNWGV
                     SLEKSIKWMEEEIVPYYALGEFKQRDGE"
     gene            4850..5389
                     /locus_tag="ACOIAF_003259"
     CDS             4850..5389
                     /locus_tag="ACOIAF_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HGZ2670254.1"
                     /translation="MEKELVFAGFGGQGVLTLGLIVAESALELGKQVTWMPAYGPTMR
                     GGKAYSVVKFSDESIGGPDMEEIDVLVAMNKPSLDYINLVKEGGTVVINTSAIDENVT
                     LREDISVVKINCQELAQKVNNPKAANIVVLGALIAKTGLFEKELALKTMCDFFEEKGK
                     GKFNVQNEAAFMEGYNNAI"
     gene            5513..7612
                     /locus_tag="ACOIAF_003260"
     CDS             5513..7612
                     /locus_tag="ACOIAF_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HGZ2670255.1"
                     /translation="MSLEKLIRPKSIAIVGVTDKLGFGRSAALSIVKSKETDRVYYVN
                     PKREELFGRKCYKTIQEVPEVVDCVVVCTPRNVVPSVLKDSGELGTKAAVVYASGFAE
                     EGTEEGTDLENQLIEISNTYDMKILGPNCMGLLNCIDKVNLWAGGSKWDLDTKKPGIG
                     IVGQSGFITAEIVSSDYFNISYGFSTGNGNIVTLEDAVDFLIDDDYASVIAIYLEGLK
                     NPQKFIDALKRAAQKRKPVIILKSGRSEKGAISAASHTGNLAGSSKAFESIFEKYGVI
                     SVENLEQFMCLAQAFSVLDGNLPANSNFAAINFSGGENTICADLAEENGVELAEISSE
                     TKEEMKKYLPGFATPKNPLDATTALFHEKDMIVGLLHTFEKDDSVGFTMVGANIRDEE
                     NEMHETLCKAVSEAREQGLKKPVFAVPTLEGNRYLDYRTRLEDNQVPIMSSVGTTFTC
                     FNKMAKFIDYDYSKRTLEFKAVKKRESNNVVALSELDSKIEMKKYGVPVPGQGNAKSI
                     DELDEILKYIKYPVVLKINSSEILHKSDVGGVKIGIKNRDEAVDAYNEILTNVKKAKP
                     DANIDGILVQEMVESGIEIIIGITNDDQFGPLLLVGLGGVFVEVFKDTTLYPLPINHD
                     EAIMMLKKLKSFKLLNGYRGSEPCDIDALADMMVKLGKYAYENKDEVKEIDLNPVFVY
                     PKGKGVCAVDALIVKYK"
     gene            complement(7879..8394)
                     /locus_tag="ACOIAF_003261"
     CDS             complement(7879..8394)
                     /locus_tag="ACOIAF_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HGZ2670256.1"
                     /translation="MISAKMEKLLNEQINHELYSAYLYLSMSSYLEAEGLKGFSNWFY
                     VQYKEETDHAMFFYKYLHNVGGKVQLDAIPMPDSDFTSAMDILERTLAHEKKVTALIN
                     NLAAVANSESDFRTSQFLLWFISEQAEEETNCEDNIKRVKLAGEGGLFFVDQEFANRT
                     YAAPTNPPVTI"
     gene            complement(8486..9697)
                     /locus_tag="ACOIAF_003262"
     CDS             complement(8486..9697)
                     /locus_tag="ACOIAF_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HGZ2670257.1"
                     /translation="MSNKQGSNIKDVKIKNKLIILKLLSTNTPMSRVDLAKSTGLTKM
                     TLSNLVTELINENMIREIESSTLNAHSSGRKPILLDISNISPCICGILIRRNICKVII
                     SDLKGNIVKQLSETYPKCLSNDVLKDIIKRMFTKIMKINKRDIIGIGISSIGPVDDIN
                     GTILNPPDFGNVSNLNIVDFIKEMSDLPIFLINDANSGALAEKMYGLGKNISNYIYLH
                     IMNGIGAGLVLENKLHTGNLGQSGEIGHTSINFSGPLCSCGNNGCLDYYTSVSNLIKR
                     IESLSHIYPNSPLINCKDFSLVKLIDEANNKDSLAMFLLDEFCTYVSYALVNTLTLID
                     CSSIIIGYDFNIPGTFIENTLLKKLTASASFSKYKKISVRHSNFGANAPLIGSIAIVS
                     YNFFNGSINFY"
BASE COUNT         3653 a         1212 c         1822 g         3084 t
ORIGIN      
        1 caattatgaa ttttgagaat ataattaaat taaaattata cacatataaa aatatactcc
       61 tgaatcaaga atgagatatt taagattcaa aatatatatt attatacgtg aataataaaa
      121 agtaagtaaa ttttataagg gaggggtaga tgtgagtagt caagaaagca aaccaaaagg
      181 aaatcttata tcaaatttta gagatgatat gagagaccct aagaaaagag caatatacac
      241 atattcaagt tctataaatt tagcgtacaa ttttataggt tactttgtat caactgttac
      301 aaatattttt ttaactgatg tggcaaaact tggtgtaatt atggcaggtg gtatgcaaat
      361 tatacaatct ttcataaaaa ttgctgtagc tcctataaca ggtacagtat ttgacaaaca
      421 accattcaaa aaaggaaagt attatccatg gcttagatat gcaccagcag cgttagcatt
      481 aacatatgga atatttttca tagttccatt aatgaaaata agtccagcat tacttttacc
      541 tttgatgaca gctttattat taatagcatc ttgtgctcag caagtagttt tcactataat
      601 ttatgctatt taccctgtag ttgctaagac acctagagac cgagttatag gctcaactac
      661 tacgaatata tttaaagaag ttggtaaatt cttagtaggt ttttcatatc cgttattatt
      721 agttttattt gcagatgtat taggtggaga aggaatgggt tatttaggta cctacttatt
      781 ctttgcagca ggatgccttt tagtattttg gttttcttca acagagataa taaaatctgg
      841 agcagaagaa gaagttcttc aaaaagaaga gaagccaaag attaaagcaa gtgaaatggc
      901 aaaagcttta ttcactaatg taccacttat gatggcattt tgtttagaat ttcttatatg
      961 tataagatct ataggattag gacctttggc tccatattat tttaaatatg taatagaaga
     1021 tgaacgtgga ttagcagtat ttttatcaat catgcctttg gtaagtgtag cattcatgtt
     1081 tttcgcacca gtgtttataa aaatctgtag agagcaaaaa ctagcttcaa taatttcttt
     1141 ttctatatgt gcactatgcc acttattggt tgcatggact ccttggggga aaactacttt
     1201 aggagtaact atgttgttgg caattggtgg aggtttttca aatgtagtat caataataaa
     1261 tcttaatttc tttgccggtt cttgtgatta tgggcattgg aaatcaggaa aagacttacc
     1321 tggcttaagt atgtcactat atccagttgc aatacaagta ggagtattac ttgcaactac
     1381 tataagaaca gttttaatga actcaatggg atatcaagct gatatggtag tcacagaagc
     1441 agttaaatca ggttttataa atatgatttc ttattctatg gcaatacctt taataatagc
     1501 tgttgttata gcaatactat atccagtaag tgacaagaaa ttaaaccaaa taagagaaga
     1561 gttaaatcaa agaaatgctt agttaaaaga aagttgagta gatggttaat ttaagctatt
     1621 aaagtaagta gacgcaatag cgtttatata aatgaaaata catatacaga gggggctttt
     1681 aaaaatgata gaaaaattat tgagaaatac cgtaaaagaa ttacatcaat atgttccagg
     1741 tgaaccaata gaaaaagtta aggaaaaata tggtgtaaaa gaaataataa aattagcttc
     1801 aaatgaaaat cctctaggac catcaccaaa agcaatagaa gctatgattg aaatgttaaa
     1861 acaaggtcaa ctttatccag agccagaagc taacgaactt agaagaaaat tagctgaaaa
     1921 gctagattta aaaccagaaa attttattgt tgctaatggt gcagataacg ttatcactct
     1981 aataggtgag gcttttataa atagaggaga tgaagttata tattgtaacc caacatttcc
     2041 atcatataga actgcaacta taaaaaatga agggatacca gtagaagtac ctttaacaga
     2101 agattataag tatgatttac aaggtatatt agataagatt acagataaaa caaaattaat
     2161 atgtgtatgt aaccctaata atccaacagg aacaattgtg gatgataaag agttagaaga
     2221 atttttaaag aaagtccctg aaaatattat aactatattg gatgaagcat atatagaatt
     2281 tttaacagtt cctaattatg tggatggact taagtatgta agggaaaatt ataacgttat
     2341 agtaacaaga actttctcta aaatatatgg tcttgcaggg cttagagtag gatatggtat
     2401 tgctaaagat gagataataa gaactttatt tacagtaaaa gagcctttct ctgcaaatcg
     2461 tgttgctata agtggagcaa cagcagcact tgatgatgaa gagtttataa aagaaacata
     2521 tgaattaaat agagttggaa tggcttactt taaagaagag tttacaaaaa tgggatttga
     2581 tgttgtagat tcacaatcaa atttccttta tgtagatatg aaaacagata tacctaagtt
     2641 atttgaagac ttgaaaaaaa gagggtttgt aataagacca agtgctacac atgcacgtgt
     2701 aagtattgga actatggaag aaaataggaa atttgtagca gttttaaagg aaatattaaa
     2761 tattaaagga gagccaagta ttggataata gatagaaata ttatgaaacg gggtgtagat
     2821 atgaacttaa aaataaacgc agagagatgt aaaagttgtg aatactgcgt tatatcttgt
     2881 aaaaaaggag cattaaaaat atcaagtaag ataaataaag aaggttatgc tcatgtagaa
     2941 gttgatgaga gtaaatgcat actttgtgga atttgctatc aggtgtgtcc agataatgtt
     3001 tttgaaataa ttaaagaaga agcatgtcaa gaggcttaga aggagttggg ataatatgaa
     3061 aaagatgtgg aaggggaacc acgccattgc agaagcagcc ctaagaggag gttgtgaatt
     3121 ttatgcagga tatccaatta caccacaaac agaggtaatg gaatttttat cacatagaat
     3181 gtctgaactt ggaagaactt ttatacagtc cgaaaatgag atggctgcta tatatatggt
     3241 atatggagca tatgcaagtg gaatgaggtc tatgacgagt tcttcaggac caggaatatc
     3301 tttaaaacaa gaagggattt cttacttatg tgcaaatcat tacccttgtg taatcgtaaa
     3361 tgtacagaga tgggggcctg gtcttggctc attggactca gcacagacag actatctaag
     3421 agatactaga gggggaggaa atggagatta tcatttgata gtttatgcac caaacagtat
     3481 tcaagagact gtagatttga tgtataactc atttgatgta gcggagaagt acagagtgcc
     3541 tgtcgaaatt ttgacagaag cagctttagg tcaaatgatg gaaccagtag aatttccaga
     3601 gtttaagaag agagaaggag atttaggttg gacttatgat ggaagtaata gagaccatgc
     3661 aaaggttgca gataaccaaa agccaacttt ttgtatggag aaacgtatga gaataactga
     3721 aaatgaacaa caatgggaag attatcaagt agaagatgca gaatatgtgt ttgtagcgtt
     3781 tggtatacca tcaagaacaa ctatgaatgc tgttagaaga cttagagaac aaggtgaaaa
     3841 agttggaata ataagaccaa taacagtatg gcctttccca tataaagcat ttgaaaaggt
     3901 aagtaaagat gtaaaaggat ttatctcagt agaatcaaca gacacaggtc aattagtaga
     3961 agatgttgct ttagcctcaa agaaagtatg taaagaaaat gtacctgtgt atggtttatt
     4021 tagtggaaat catataccaa aaacattgca agtaatggac acttatagca aaataaaatc
     4081 tggcgaaatc aaggaggtgt tctaatatgg ctacagaaaa attaacacct agcgttgttt
     4141 caataccaaa caagttttgc ccaggttgtg gtcatggaat agtaaatcgt ataatagcag
     4201 aggtaataga agaacatgga tatgaaaaaa atcatgtatt aacattagga gttggttgtg
     4261 tctgcaatat gaattttagc tggaatggtg ataagatgca aactgcacat ggtagagctt
     4321 catctacagc tataggagtt aaagtagcac ttccagatac attagtaatg acttaccaag
     4381 gtgatggaga tgcttatgtt ataggtcttt ctgaaacttt aaatacagca tatagaaatc
     4441 ataatgtgac tgtatttgta ataaacaata ataattttgc aatgactggt ggacaaatgt
     4501 catggaccac tatgccgggt caagtcacaa caacaagtgt aaatggaaga gatataaaga
     4561 ctactggtag tccactgaaa gttcctgaga tggtggcaaa cttctttgat gtagcatatg
     4621 tagcaagagg ttcagttcat agcccaaagg aaataattaa tctaaaaaaa tatataaaaa
     4681 atgcaataga agctcaatta aatggagagg gatattcctt agttgaaata ttagcacctt
     4741 gcccaactaa ctggggtgta tcactagaga aatctatcaa atggatggaa gaagaaatag
     4801 tgccatatta tgctcttggc gaatttaagc aaagggatgg tgaatagtta tggaaaaaga
     4861 gctagtattt gcagggtttg gtggtcaagg agttttaact cttggtctga ttgtagctga
     4921 gtcagcactt gaattgggga aacaagtaac ttggatgcct gcatatggtc caactatgag
     4981 aggtggaaaa gcctatagtg tagtaaagtt ttctgatgag agtataggtg ggcctgatat
     5041 ggaagaaata gatgttttag tagcaatgaa taaaccatct ttagattata taaatttagt
     5101 taaagaaggt ggaacagtag ttattaatac atctgcaata gatgagaatg taacattaag
     5161 agaagatata agtgtagtta aaataaattg tcaggaatta gctcaaaaag taaacaatcc
     5221 aaaagcagca aatatagtag tacttggagc attaattgct aaaactgggt tatttgaaaa
     5281 agaattagct ttaaaaacta tgtgtgattt ctttgaggaa aaaggaaaag gaaagtttaa
     5341 tgttcaaaat gaagcagcat tcatggaagg ctataataac gccatttgat aaattaccct
     5401 tatcttaagt gatttatctc aagatttaat aatctttagt gccaagagaa acttcatctt
     5461 gagataaaat tcacttaaaa tcacaactat aaacatatag gagcgtgaat ttatgagttt
     5521 ggaaaaactt attagaccaa aaagtattgc tatagtagga gtgactgata aacttggttt
     5581 tggaagaagt gcagcactta gtatagtaaa aagtaaggaa acagatagag tatattatgt
     5641 taatcccaaa agagaagaat tgtttgggag aaaatgttat aagacgatac aagaagttcc
     5701 agaagtagta gactgtgtag tagtatgtac gcctagaaat gtagttccat ctgtattaaa
     5761 agattctgga gagttaggaa caaaggcagc agtggtatat gctagtggat ttgcagagga
     5821 aggtacagag gaaggtacag atttagaaaa tcaacttata gaaatatcaa atacatacga
     5881 tatgaaaata ttgggtccaa actgcatggg gttgctaaat tgtatagata aggttaatct
     5941 atgggcagga ggttctaaat gggatttaga tactaaaaaa cctggtatag gaattgtagg
     6001 tcaaagtgga tttataactg ctgaaattgt ttcaagtgat tattttaata tatcttatgg
     6061 tttttcaaca ggaaatggaa atatagttac actagaagat gcagtagatt tccttataga
     6121 tgatgattat gcatcagtaa tagcaattta ccttgaaggt ttaaaaaatc ctcaaaaatt
     6181 tatagatgct ttaaaaaggg cagcacaaaa aagaaagcca gtaataatat tgaaatcagg
     6241 aaggtcagaa aaaggtgcta tttctgctgc ttctcataca ggaaatttag ctggttcgag
     6301 taaagctttt gaaagtattt ttgaaaaata tggagtaatt tctgtggaaa atttagaaca
     6361 gtttatgtgt ttagcacaag cttttagtgt tttagatggt aatttacctg caaattctaa
     6421 ttttgcagca ataaacttct caggaggaga aaatactatt tgtgcagatt tagcagaaga
     6481 aaatggtgta gaacttgcag aaatatcttc tgaaactaaa gaagaaatga aaaaatatct
     6541 tccaggattt gcaacaccta aaaatccact agatgctaca acagcattat tccatgaaaa
     6601 ggatatgata gtaggtttat tacatacctt tgaaaaggat gatagtgttg gatttactat
     6661 ggtaggtgca aatataagag atgaagaaaa tgaaatgcat gaaacccttt gtaaagctgt
     6721 aagtgaagct agagaacaag gattgaaaaa acctgtgttt gcagttccta cacttgaagg
     6781 aaatagatat ttagattata ggactagatt agaagataat caagtgccta taatgtcatc
     6841 agttggaact acatttactt gttttaataa aatggctaag tttatagatt atgattattc
     6901 aaaaagaact ttagaattta aagctgttaa gaaaagagag tcaaataatg tagtagcatt
     6961 atcagaatta gatagtaaga ttgagatgaa aaaatatgga gtaccagttc caggacaagg
     7021 taatgcaaaa agtattgatg aattagatga aatcttaaaa tatattaagt atcctgttgt
     7081 tttaaaaata aattcttctg aaatattgca caaatctgat gttggaggag ttaaaatagg
     7141 aattaagaat agagatgagg cagtagatgc atataatgag attttaacta atgtaaaaaa
     7201 agcaaaacca gatgctaata ttgatggtat attagttcaa gaaatggtag aaagtggtat
     7261 agaaattatt attggtataa ctaatgatga ccaatttgga ccactgttat tagtcggttt
     7321 aggaggagta tttgtagagg tatttaaaga tactacatta tatccattac ctataaatca
     7381 tgatgaagct attatgatgc ttaaaaaact aaaatcattt aagcttttaa atggctatag
     7441 aggaagtgag ccttgcgata ttgatgctct tgcagatatg atggtcaaat taggaaaata
     7501 tgcttatgaa aataaggatg aagtcaagga aatagactta aatccagtgt ttgtatatcc
     7561 taaaggaaaa ggcgtatgtg cagtagatgc cttaatagtt aaatataaat agtttataag
     7621 ttttggtatt caaccataat taaagcaccg actgaaaatc acattttgta agttagatag
     7681 tgtgattttc agtcacccca aatttaattt aaaaatattt taaaatgaaa gtatagtttt
     7741 aagtttcatt gtgaaatagc actgtataaa caataaaata ctacgtataa aacgaataat
     7801 aaaaggtgtt ttaatgataa gaatcaatct tgattttcat taaaacacct tctttattat
     7861 attaaaaatt tttaaacgtt atattgtcac aggtgggttt gttggagctg catatgtacg
     7921 attagcaaat tcttggtcaa caaagaatag accaccctct ccagcaagtt taacacgttt
     7981 tatattgtct tcacaatttg tctcttcttc tgcttgttca gatataaacc aaagtaagaa
     8041 ttgagaagtt ctaaagtctg attcagagtt tgctacagct gctaagttgt ttattaaagc
     8101 tgtaaccttt ttttcatgag ctaacgtacg ctcaagaata tccattgcag aagtaaagtc
     8161 tgaatctggc attgggatag catcaagctg aacctttcct cctacattat gtaagtattt
     8221 gtaaaaaaac atagcgtggt ctgtctcttc tttatattga acataaaacc aattagaaaa
     8281 tcctttaagt ccttctgctt caaggtaaga agacatagaa agataaagat acgcagaata
     8341 taattcatgg ttaatctgct cattaagtaa tttttccatt ttagctgaaa tcatttttat
     8401 acctccaaat tttaaatgct cttataaaat ataataatat taaatggaaa aatattcaat
     8461 aaaagtgttt aaatatttta catatttaat aaaaatttat actaccatta aaaaaattat
     8521 aacttacaat ggctatagag ccaattaatg gtgcatttgc tccaaaattt gaatgcctta
     8581 cacttatttt tttatattta gaaaaacttg ctgaagcagt caattttttt aataatgtat
     8641 tttctataaa tgtaccaggt atattaaagt catagcctat tattatagaa ctacaatcaa
     8701 tcaatgtaag tgtattaact aatgcatatg atacgtatgt acaaaattca tcaagcaaaa
     8761 acatggcaag actatcttta ttatttgcct catctattaa cttgactaaa gaaaaatctt
     8821 tacagttaat taatggggag ttaggataaa tatggctcaa tgattcaatt cttttaatta
     8881 gattactgac acttgtataa taatctaaac atccattgtt accacaactg cacaatggtc
     8941 cactaaaatt aattgatgta tgtcctattt ctccactttg tcctaggttg ccagtatgaa
     9001 gtttattttc tagaactaga ccagctccaa taccattcat tatgtgtagg tatatataat
     9061 ttgatatatt tttacctaaa ccatacattt tttctgctaa tgcacccgaa ttagcatcat
     9121 ttatcaaaaa tattggcaaa tcagacattt cttttataaa atctactata tttaggtttg
     9181 atacatttcc aaaatctggg ggatttagta tggtaccatt tatatcatct actggaccta
     9241 ttgaagaaat accaattcca attatatctc tcttgttgat tttcattatt ttagtaaaca
     9301 ttctttttat tatatctttt aaaacatcat ttgatagaca tttagggtaa gtttcagaaa
     9361 gttgttttac aatattacct tttaaatctg aaataattac cttacatata tttcttctta
     9421 ttaggattcc acaaatacaa ggcgatatat tagaaatatc taataaaatg ggtttacgac
     9481 ctgatgaatg tgcatttaaa gttgatgact ctatttccct aatcatattt tcattaataa
     9541 gttcagtaac tagatttgat aaagtcattt tagtaagtcc tgtagactta gctaaatcaa
     9601 ctctggacat aggtgtattt gtagaaagta atttcaatat tattaactta tttttaattt
     9661 ttacatcttt gatgttagaa ccttgtttgt tactcatcat aaatctcctt aaataaagtt
     9721 aagtgtttta tttattttca agtatactat caccttataa tagagtaaca t
//
LOCUS       DBGJFF010000140         9490 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.143, whole genome shotgun sequence.
ACCESSION   DBGJFF010000140 DBGJFF010000000
VERSION     DBGJFF010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9490
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.143"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(92..1846)
                     /locus_tag="ACOIAF_003263"
     CDS             complement(92..1846)
                     /locus_tag="ACOIAF_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp23"
                     /protein_id="HGZ2670258.1"
                     /translation="MYIMNKKFKKFSKKLSILILVLTMFLFNNLTANAQIKEDSIIGI
                     DRYETAGLIADRQTYDTVILVNGDKNLSDGLSSSGLAGAINAPILLTKKSEIPKATLN
                     RLDNKSLNVVKKVYIIGGYNTIENSVEKDIKRKGIQVERINGNNRIETSYNVAKKINE
                     VSSVKEVMLTNGFVGEADAMSIAPVVAKNKGAIILTDGKSVPFKTESLNVYAIGGKSA
                     INEDLVNETKATRIGGNDRFETNKKVMEKFYNGATDFYITNGYQLVDALTLSPLAKEK
                     PIVLVADGSNKGILKGAKSITKVGGIDANIYKQCLDMIKYNNMDMTPNILKHLTSVEG
                     NEVSEILIESDNLGVVVEKTNTNKFEFDYVSVTNEKNCTFSVYKENSSNNIKNGKLVV
                     SAKKKIDKKDSDLQDMNGDNMINANKDRMVNVIKIGVPDKMYSNFKVDVESGSVELYN
                     IKGGATVDVNDGIANIVDNNVTNPFNINTNSGISGVTAEAISSEIKFRANNGIVNVTA
                     TNINGNISLFGSDNKDTFDGIFKLNLKKEPSNLHLKLIGNGINKLPEGWSKDYILGNG
                     QPIIEVKNNGINNISIGE"
     gene            complement(2064..2954)
                     /locus_tag="ACOIAF_003264"
     CDS             complement(2064..2954)
                     /locus_tag="ACOIAF_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HGZ2670259.1"
                     /translation="MAKLTLEHIKGNTYYIPLPTIVGVYVDGKDAILIDSGNNKDTAR
                     QVLRLLEEHNLNPKLIINTHSNADHIGGNAYLKNQTKCKIATTKIEGYFTENPILESA
                     FLYGGYPSKALKNKFLLAKESEVDYIIPSNGKIVDTELEAISLPGHYFEMIGVRTPDN
                     VLFVADSLTPENIITKYHFFFLLDIDSQFKTLEKLRMLEADFFVPSHSVKTTDIKSLI
                     NINKKKMEEIIDNIKKVCYEPVMIDEVIEKMCDLYNVKLDANQYVLVGSTIRSYITYL
                     YENNMVEYIFDGGKMMIKAV"
     gene            3103..3963
                     /locus_tag="ACOIAF_003265"
     CDS             3103..3963
                     /locus_tag="ACOIAF_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HGZ2670260.1"
                     /translation="MLLKKDRITFSEILILIVGCILMAISLNLFFNPHAIAAGGITGL
                     GVVLNSLFGVELWIVNLLLNVPLFIFAYKILSKKDCFKTVLGIIFLTIALKLTANMAT
                     LDITNDMYLAIISGSILMGVGQGLIFRINGSTGGTDLMALLLNKYFPTFSIPVLMGIV
                     DCVVVVLSGIVNRQVEIALYSTVALYILVKVSDLLIEGFNYSKSFTIISDLSKDISKK
                     IMEDLDRGATILKGEGAYTGENKNVLLVVVEKKEVVELKKLVKNVDPNAFIIITDIHE
                     ALGNGFKKIE"
     gene            4419..4985
                     /locus_tag="ACOIAF_003266"
     CDS             4419..4985
                     /locus_tag="ACOIAF_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HGZ2670261.1"
                     /translation="MSVSLQKGQRVSLVKDNNPVKNLVVGLGWDMNKLGKKNYDLDAF
                     AIALTNQDKMNVRGDLVYFGNLKHPSKAIIHTGDNLTGKGEGDDEQIIVNLEDIPEYV
                     HKIVFGVSIYKAKKRDQDFGQMNNSFIRLIDSNSKQELFKYNLQEDFSGKVTVLAGEI
                     YRRNEEWKFNALGIGQNEELRELINTYK"
     gene            5025..5606
                     /locus_tag="ACOIAF_003267"
     CDS             5025..5606
                     /locus_tag="ACOIAF_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HGZ2670262.1"
                     /translation="MGITLAKGQKVSLTKSNPGLKKVIVGLGWDINKYDGGFDFDLDA
                     SAFLTGTDGKVTNDGDFIFYNNLKHASGAVEYMGDNRTGVGDGDDEQINVDLSKIPQN
                     IAKISFSVTINEAITRRQNFGQVENSYIRIYNEETNEELIKYELGEDFSIETAIVVAE
                     LYRHNGEWKFNALGSGFEDGLAGLCKNFGVNIG"
     gene            complement(5663..6580)
                     /locus_tag="ACOIAF_003268"
     CDS             complement(5663..6580)
                     /locus_tag="ACOIAF_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGZ2670263.1"
                     /translation="MTIRSLEIFVKVAECGKMSEVARNMYITQSSVSQAISEIEKEYG
                     VKLFDRISKKLYLTEAGKKLLGYGRHLLAVNEEMNDCMKHCAKNIRIRVGATVTVGTC
                     VISPIMLELYKVNPLIEPEVFVEDTRLIAKKLLNSELDIAIVEGKIKHPDIVTKSIIN
                     DNLVLICSHKHEFYKRDSIKVSELSNQPLIMRELGSGTRAQLEKQLKELKIPMNIRWS
                     CYNSEAILRAVVDNFGIAVISELLIEDYLKKHLLWACDIEGINLHRTFDIAYHRSKFF
                     TENISAFFDISVEYGKKQARKKSRSVNSL"
     gene            6710..8341
                     /locus_tag="ACOIAF_003269"
     CDS             6710..8341
                     /locus_tag="ACOIAF_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HGZ2670264.1"
                     /translation="MKVLIIGGVAAGTKTAAKIKREMGDNCSVTILNKGDDISYAGCG
                     LPYYVGKIIEDKSSLIVNTPESFSKLTGAEVRCGVEVTSIDRNAKQVTLKTLSTGEKE
                     NLSYDKLVIATGADPIKPPIPGIDLPGVFFMRTPNDAIKLRDSIENGIKRAVVIGGGF
                     IGLEIAENLSAMGIRVSVVDMAEHVMPGFDTDFAEYVENHMADKGIMIFTGDQVVGIE
                     GENKVEKLRTKNRAIKTDLVVMSVGIRPNTGFLKDTGLEFAPNNTIIADEYMLTNDKD
                     IYVVGDCAFVKNSLTNKPTWSPMGSSANHEGRICAQNISGKSKTYNGVLGTTVVKLPE
                     LNAGKTGLSKEIAEKEGYNVCSVTIATDDKAHYYPGASNFIIRMVAEKNTRKLLGVQV
                     MGPGAVDKIVDIAATAITLGADLYSLESMDLAYAPPFSTAIHPFVVAVNVLQNKLNGE
                     LDSVLLDEIEDFDSWTKLDVSKAPSIPSLRYIPVGEINGEIEGLAKDEKILLICAKGK
                     NSYMAQNRLRRFGYTNTKVLEGGILFYPNLKVMEE"
     gene            8344..9291
                     /locus_tag="ACOIAF_003270"
     CDS             8344..9291
                     /locus_tag="ACOIAF_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HGZ2670265.1"
                     /translation="MSNVTAEQKKEVKGRGFLPSRDGEHFAARIITVNGVINASQAKK
                     IAEAAEKFGNGQLAFTTRLTVELPGVKFEDIEALSEFIASENLITGGTGSRVRPVVAC
                     KGTVCVHGLADTQALAAEIHEEFYKNWYDVKLPHKFKIGVGGCPNNCIKPDLNDLGIV
                     GQRVPDYDSELCVGCKKCAVVEVCPVKAAKLTDKGKLEIDSNLCNNCGKCIESCNFDS
                     IEEKESGYKVYIGGKWGKSVRPGTQIDRLFSKEELMTLIEKAILLFREQGNTGERFGI
                     TIDRIGADKFIEMLLSDEVLERKEEILEALLHLTGGAVC"
BASE COUNT         3327 a         1334 c         1372 g         3457 t
ORIGIN      
        1 ttaaaaaata attttttatt taaagaatat atataaatat tttaaaattc tataattttt
       61 aaaactatat aaactttatt ttcatatgtt tttattctcc aattgatata ttatttatgc
      121 cattattttt aacttcaata attggttgtc catttcctaa aatataatct ttagaccaac
      181 cttctggcaa tttatttatc ccatttccaa ttaacttcaa gtgtaaattt gatggttctt
      241 ttttcagatt taatttaaat attccatcaa aagtatcttt attatcgctt ccaaataaac
      301 ttatattgcc atttatattc gtagctgtta catttacaat tccattgttt gctctaaatt
      361 ttatttcaga tgatatagct tcagctgtta cacctgaaat cccactatta gtatttatat
      421 tgaaaggatt tgttacattg ttatcaacaa tgttagcaat cccgtcattg acatcaacag
      481 tagctcctcc tttaatatta taaagttcta ctgaaccact ttcaacatct actttaaaat
      541 tagaatacat tttatcggga actccaattt ttatcacatt taccatccta tctttatttg
      601 catttatcat attgtcgcca ttcatatctt gcaaatcact atctttctta tctatttttt
      661 tcttagcact tacaactagc tttccatttt ttatattatt agaagaattt tctttatata
      721 cagaaaaagt acaatttttt tcattagtta cacttacgta atcaaactca aatttattgg
      781 tattcgtctt ttcaacaacc acacctaaat tatctgattc tataagtatt tcagaaactt
      841 catttccttc aactgaagtt aagtgtttta aaatatttgg agtcatatcc atattattat
      901 atttaatcat atctaaacac tgtttatata tattggcatc aatacctcca acttttgtta
      961 tacttttagc tccttttaaa atacctttat tacttccatc agcaactaaa acgattggtt
     1021 tttctttggc taaaggagat aatgttagtg catctactaa ttgatatcca tttgttatat
     1081 aaaaatctgt cgctccatta taaaactttt ccattacctt tttattggtc tcaaatctat
     1141 catttcctcc gattctagtt gctttagttt catttactaa atcctcattt atcgcagatt
     1201 tgccacctat agcgtaaaca tttaaacttt cagtcttaaa tggaactgat ttcccatctg
     1261 taagtattat agctccttta ttttttgcaa ctacaggtgc tatactcata gcatcagcct
     1321 ctcctacaaa tccatttgta agcattacct cttttacact acttacttca tttatttttt
     1381 tagctacatt ataactagtt tcaattctat tgttaccatt tattctttcc acttgaatac
     1441 cttttctttt tatatctttt tctactgaat tttcaatagt attataacca ccaattatat
     1501 atactttttt tacaacattt aaactcttat tatctagtct atttaaagta gcttttggta
     1561 tttcactttt tttagtcaaa agtattggtg cgttaatagc tccagctaat ccacttgatg
     1621 aaagcccatc agataaattt ttatctccat taactaatat tacagtatca taagtttgtc
     1681 tatccgctat aagtcctgct gtttcatatc tatcaattcc tattattgaa tcttctttaa
     1741 tctgtgcatt tgcagttaaa ttattaaaaa gaaacatagt caaaactaaa attaatatac
     1801 ttagtttttt actgaatttt ttgaattttt tattcataat atacactatt cctcctgtaa
     1861 attaaggtct ttaatttgct tataaattat ccccttattt acaaataata acataatatt
     1921 atagtaacgt atatattacc ttaaaattat cagtaacttt atatcaatca tcttagctta
     1981 tttatataaa tttttttata ttacttttca aataacagtc atactaaaac aatttcatca
     2041 tataatttga aaattattac tatttacaca gcttttatca tcatcttacc accatcaaat
     2101 atatattcaa ccatattatt ctcatataaa taagttatat aagaacgtat tgtacttcca
     2161 accaacacat attgattagc atcaagcttg acattgtaca aatcacacat tttttcaata
     2221 acttcatcaa tcataactgg ctcataacaa acctttttaa tattatctat tatttcttcc
     2281 atcttcttct tattaatatt aattaaactt tttatatctg tagttttgac agagtgactt
     2341 ggaacaaaga aatcagcctc taacattctc aatttttcta gtgtcttaaa ctgtgaatct
     2401 atatccagta ggaagaaaaa atgatattta gtaattatat tctctggtgt aagactatct
     2461 gcaacaaaca aaacattatc tggagttcta acaccaatca tttcaaaata atgacctgga
     2521 agagaaatcg cttcaagttc tgtatctaca attttgccat ttgaagggat aatataatca
     2581 acttcacttt cctttgctaa taaaaattta ttcttcagtg ctttacttgg atagccacca
     2641 tataaaaatg ctgattctaa tattggattt tctgtaaaat atccttcaat tttagtcgta
     2701 gcaatcttgc attttgtttg attcttcaaa tatgcatttc ctcctatatg gtctgcattt
     2761 gaatgagtgt ttattattaa tttaggattt aaattatgtt cttctaataa tcttaaaact
     2821 tgtctagctg tatccttatt atttccactg tcaattaaaa ttgcatcctt accatctacg
     2881 tatacaccta ctattgtagg gagtggaata taatatgtat ttccttttat atgttctaga
     2941 gttaatttag ccattttata acctcccttt aaatttactt ttaattatag cattaatttt
     3001 ctgaaaattc caatcaaatt gtttttataa tatattttta tttcttttat taaaaattgg
     3061 aaaaactata taatatatat tataatctat atataaggag tgatgttatt gaaaaaagat
     3121 agaataactt tttcagaaat tttaatatta attgtaggct gtatattaat ggccatttct
     3181 cttaacttat tttttaatcc acatgcaatc gctgctggtg gtataacagg acttggagtt
     3241 gttttaaatt cattattcgg tgtagaactt tggattgtca atttactttt aaatgttcca
     3301 ttattcatat ttgcttacaa aatactttca aaaaaagatt gttttaaaac ggttttagga
     3361 ataatttttc ttaccatagc acttaagctt actgcaaata tggctacatt agatataaca
     3421 aatgatatgt atcttgcaat tatatctggt tctattttaa tgggagttgg tcaaggactt
     3481 atttttagaa taaatggctc tacaggtgga actgatttaa tggcactcct actaaataaa
     3541 tattttccta catttagtat acctgtttta atgggtattg tagactgcgt tgtagttgtt
     3601 ttatcaggta tagtaaatag acaggttgaa attgcacttt attcaactgt ggctttatac
     3661 attttagtaa aagttagtga cttgcttatt gaaggattta attattccaa atcattcact
     3721 attatatctg acctttcaaa agatataagt aaaaaaataa tggaagattt agatagaggt
     3781 gcaactattc taaaaggaga aggtgcatat actggagaaa ataaaaatgt acttttagta
     3841 gtagttgaaa agaaagaagt tgtcgaatta aaaaaattag taaaaaatgt cgaccctaat
     3901 gcttttatta taattacaga tatacatgaa gctttaggta atggatttaa aaaaatagaa
     3961 taactaacaa ataaatagca agtataatta acatatacaa attcaacttt ttatatgtta
     4021 tattatactt gctatttata tattatactt gctatttata tattataatt gttgttttta
     4081 agataaaaat taaatgtatc aattattgta ttatacaagt atttccaaac ccgttctcat
     4141 ttagatagta tgcttagaaa ttttagaatt attgagtcta actttattac ttacttcact
     4201 ttataacaat taaatacaaa tttaaatatt ataaaactac aaaataattt aaagcatata
     4261 atgtcaatat gtttaaatta taaaatattc atgtagttat ataataaata atttataaat
     4321 atagccaact tagcctaaat acgtatataa tatagctaat atggttctta tacttgatac
     4381 catcaataaa actaaaaata taaaaaggag aacttaaaat gagtgttagt ttacaaaaag
     4441 gacaaagagt tagcttagta aaagataata atcctgtaaa aaatctagta gtaggcttag
     4501 gatgggatat gaataaattg ggtaaaaaaa attatgacct agatgcattt gcaatagcat
     4561 tgactaacca agataaaatg aatgttagag gtgacttggt ttattttgga aatcttaaac
     4621 acccttctaa agctattata cacactggag ataacttaac tggaaaaggt gaaggtgatg
     4681 atgaacaaat aattgttaat ttagaagaca tacctgaata tgtacataaa atagtttttg
     4741 gagtttctat atacaaagct aaaaaaagag accaggattt tggtcaaatg aacaactctt
     4801 ttataagatt aattgattca aattccaagc aagaattatt taaatataat cttcaagaag
     4861 acttttctgg taaagtaaca gttttggcag gagaaattta tcgtagaaat gaagaatgga
     4921 aatttaatgc tttaggaatt ggtcaaaatg aagagcttcg tgaattaatt aatacatata
     4981 agtaataatt acaataaaaa atttataatg aggagttggt ttaaatgggt ataactttag
     5041 caaaaggaca aaaagtaagt ttaacaaaaa gtaaccctgg tctaaaaaaa gtaatagtag
     5101 gactaggatg ggatattaat aaatatgatg gtggctttga tttcgattta gatgcctctg
     5161 catttttaac tggaactgat ggaaaagtaa cgaatgatgg agactttata ttctataata
     5221 atttaaaaca cgcttctgga gctgttgaat atatgggtga taatagaaca ggtgttggtg
     5281 atggtgatga tgaacaaata aatgtagact tatctaaaat acctcaaaat attgctaaaa
     5341 tatcatttag tgttactata aatgaagcaa ttacaagaag acaaaatttc ggacaagttg
     5401 aaaattcata tataagaata tataatgaag aaactaatga ggaacttatt aaatatgaat
     5461 tgggtgaaga ttttagcata gaaacagcaa tagttgtggc agaactatat agacataatg
     5521 gagagtggaa atttaatgct ttaggttctg gatttgagga tggacttgca ggattgtgta
     5581 aaaattttgg cgttaatata ggctaatata tctaaataaa aataagagta tattgatata
     5641 ctcttatttt tatttagata tattataaac tgtttacact tctactcttc tttctcgctt
     5701 gttttttccc atattctact gaaatatcaa aaaatgctga aatattttct gtaaaaaact
     5761 tgcttctatg atatgcaata tcaaatgtcc tatgtaaatt aataccttca atatcacaag
     5821 cccacaaaag atgctttttt aaataatcct caataagtaa ttcggatata actgcaatgc
     5881 caaaattatc tacaactgct cttaatatag cttcagagtt ataacatgac catctaatat
     5941 tcattgggat ttttaattct tttaattgct tttctaattg tgctcttgta ccacttccca
     6001 attcacgcat aattaatggt tgatttgaaa gttctgaaac ttttatagag tctcttttat
     6061 aaaactcatg cttgtgtgaa catataagta caagattatc attaataatg ctttttgtaa
     6121 caatatctgg gtgcttaatt tttccttcta caatcgcaat atctaattca ctattaagta
     6181 atttttttgc aatcagtctt gtatcttcta caaatacttc aggttctata agaggattta
     6241 ccttgtataa ttctaacatt attggactta ttacacatgt accaacagtt actgttgccc
     6301 caactcttat acggatattt tttgcacaat gcttcataca atcattcatt tcttcattta
     6361 cagctaaaag atgcctccca tatccaagaa gttttttccc tgcctcagtt aaatataatt
     6421 tttttgaaat tctatcaaat agcttaacac catactcttt ttctatttct gaaatagctt
     6481 ggctaactga tgactgggta atatacatat ttcttgctac ttcactcatt ttaccacatt
     6541 cagcaacctt aacaaagatt tcaagactac gaattgtcat attatcacaa ccttagcaaa
     6601 cattagtaaa tgcttatgat tttattaata ttttagtatt ttacaaatat attgtcaaga
     6661 agtataattt tattatagag ttataaagta gattggagtg tgatatattg tgaaagtatt
     6721 aattataggt ggagttgctg ctggaacaaa aactgctgca aaaataaaac gtgaaatggg
     6781 tgataattgc tctgttacaa tactaaataa aggtgatgat atttcttatg caggttgtgg
     6841 tcttccatat tacgttggta agataattga agataaatca tctttaatcg taaatacacc
     6901 tgaaagtttt tctaaactaa caggagcaga agttcgttgt ggagttgaag ttacatctat
     6961 tgatagaaat gcaaaacaag taactttgaa aacgctttca acaggagaaa aagaaaatct
     7021 aagctatgac aaacttgtaa ttgctacagg ggcagaccca ataaaaccac caatacctgg
     7081 aatagattta cctggagtat tctttatgcg tactccaaat gatgctataa aactaagaga
     7141 ttctattgaa aacggtatta agagagctgt tgttattggt ggaggtttca ttggtcttga
     7201 aattgctgaa aatctttctg caatgggtat acgtgtaagt gttgttgata tggctgaaca
     7261 tgttatgcct ggatttgata ctgattttgc tgaatacgtt gaaaatcata tggctgataa
     7321 aggcataatg atttttacag gagaccaagt tgtgggtatt gaaggtgaaa ataaagttga
     7381 aaaacttcgt actaaaaata gagctattaa aacagattta gtggttatgt cagttggaat
     7441 tcgtcctaac acaggttttc taaaagacac aggtttagaa tttgctccta ataatacaat
     7501 tattgcagat gaatatatgc ttacaaatga caaagatata tacgtagttg gagactgtgc
     7561 ttttgtaaaa aattctttaa caaataaacc tacatggtca cctatggggt catctgcaaa
     7621 tcatgagggt agaatttgcg cacaaaatat atctggaaaa tctaaaactt acaatggtgt
     7681 tcttggaaca acagtagtaa aattacctga gctcaatgca ggaaaaacag gtctaagtaa
     7741 agaaatagct gaaaaagaag gttataatgt ttgctctgta accatagcta ctgatgataa
     7801 agctcattat tatccaggtg catctaattt tattataaga atggttgctg aaaaaaatac
     7861 tagaaaatta ttaggtgttc aagttatggg acctggagcg gttgataaaa tagtagatat
     7921 cgctgctact gcaataactt taggcgctga cctttattca ttagaaagta tggaccttgc
     7981 atatgcacca cctttttcaa cagctattca cccttttgta gtggcagtaa atgttttaca
     8041 aaataaactt aatggagaac ttgatagtgt tttacttgac gaaattgagg attttgacag
     8101 ttggactaaa cttgatgttt caaaagctcc ttctattcct agtttacgtt atattcctgt
     8161 tggtgaaatt aatggagaaa ttgaaggtct tgctaaggat gaaaaaattc ttcttatatg
     8221 tgctaaaggt aaaaatagct atatggcgca aaatcgctta agacgctttg gctatacaaa
     8281 tacgaaagtt cttgaaggtg gaatactatt ttatccaaat cttaaggtaa tggaggagta
     8341 aatatgagta atgttacagc agaacaaaaa aaggaagtaa aaggtagagg ttttctacca
     8401 agcagggatg gagaacattt tgcagcacgt ataataactg taaatggtgt aataaatgca
     8461 tcacaagcaa aaaaaattgc agaagctgct gaaaaattcg gaaacggtca gttagcattt
     8521 actactagac taacagtaga attacctggt gtaaaatttg aagatattga agctctaagt
     8581 gaatttattg ctagcgaaaa tctaataaca ggtggaactg gatcaagggt tcgtcctgtt
     8641 gtggcttgta aaggtactgt ttgtgttcat ggtctagctg atacacaggc acttgctgct
     8701 gaaatacacg aagaatttta caaaaattgg tatgatgtaa aactacctca taagttcaaa
     8761 attggtgttg gtggatgccc taataactgt attaaacctg acctaaacga tcttggaatt
     8821 gttggtcaac gtgtacctga ctatgattca gagctttgtg ttggttgtaa aaaatgtgct
     8881 gttgtagagg tctgtcctgt taaagcagct aaattaacag ataaaggtaa acttgaaata
     8941 gatagtaatc tatgtaataa ctgtggtaag tgtatagaaa gttgtaattt tgatagtatt
     9001 gaagaaaaag aatctggtta taaagtctat ataggaggga aatggggaaa atcagttcgt
     9061 cctggaacac agattgatag attattttca aaagaggaac ttatgacttt aattgaaaaa
     9121 gcaattcttt tatttagaga acagggtaac acaggtgaac gttttggaat aactatcgat
     9181 agaatcggtg ctgataaatt tatagaaatg cttttatcag atgaagtttt ggaaagaaaa
     9241 gaagagatac tagaggctct tttacacctt acaggtggag ctgtatgcta acaataaata
     9301 ttttaattgt acagataacc tatggaaaaa attttagtta agaataatta aaattctata
     9361 tatgatatat gataaatgag gctatctcaa aacacagata cgcctctttt tcattgttga
     9421 aatgataaag tatatttgag ttcatataaa aaagagtgtc tcgtaaatta taaaattcat
     9481 tttgaggcac
//
LOCUS       DBGJFF010000141         9324 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.144, whole genome shotgun sequence.
ACCESSION   DBGJFF010000141 DBGJFF010000000
VERSION     DBGJFF010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9324
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.144"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(142..795)
                     /locus_tag="ACOIAF_003271"
     CDS             complement(142..795)
                     /locus_tag="ACOIAF_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGZ2670266.1"
                     /translation="MKVDPLTTQVYDYISKKIQNGEYEANQRITESEICKSIGVSRTP
                     AREALTRLAGENLLEKIPNKGFVVKEFQEKEKLDTYSVIGVLDALAGSSALQNLTESD
                     LVKMEELTEMMAVSIKYKNYNSYLKLSNEFHDIYITKSDNQVLVNLLNSLRYNFMSKS
                     YTSDNEDELYKMLTYSNNQHIEVVKSMRENDLENVERVLREHWKTIPIAEMKKEKLL"
     gene            complement(1040..2905)
                     /locus_tag="ACOIAF_003272"
     CDS             complement(1040..2905)
                     /locus_tag="ACOIAF_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HGZ2670267.1"
                     /translation="MQSVIEYNSWDKNFKAPFGALKFDEELTICVKVNEGYNIKSISL
                     EINREEEMRTITLNEELDNDKLGKCFCGKIEKFDGTGVYFYYFKVDVEMDGQIKTLFY
                     GKNRDNGYSCEYNYSDINKYQITVYKDFKVPTWYKEGILYHIFVDRFNNGNRSGKVDN
                     PKKNSFIYGNWEDTPMYIKDSQGDVIRWDFHGGNLRGIINKLGYLKKLGVSILYLSPI
                     FEASSNHKYDTGDYKKIDPMFGDEDTFKELIDKAKEKGISIVLDGVFSHTGADSKYFN
                     MYGNYNSLGAYQSKESPYYSWYMFEEFPQKYKSWWDVKTLPNINELEHSYMDYIIYDN
                     DSVINKWVNMGIKGWRLDVADELPTKFIRELKKELKKADDDSILIGEVWEDASNKISY
                     GQRRSYLLGEELDSVMGYPFRNNMFSFLKGEINSYELCNRYMQIKENYPKESFRSNLN
                     LIGTHDVTRAKTELNDDVDLVKLAVAIQMTFEGVPYIYYGDEAGLCGGVDPDNRRTYP
                     WKNEDEDMLNFYRDVIKIRNKNKLLSSGNTEFIYTNNDSVFSFIRVNENNDRMLILVN
                     RSENVESISLCIESSFIEEIPIKYSLKNANSTIQIENNELKVDLDSKSFRIFRVN"
     gene            complement(2910..5330)
                     /locus_tag="ACOIAF_003273"
     CDS             complement(2910..5330)
                     /locus_tag="ACOIAF_003273"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HGZ2670268.1"
                     /translation="MVTISKKKFKQKFEVKMYSLYAQSIKEATDEQLLNVLCSLLKDE
                     IAKKWVATKLDKKKEVYYFSLEFLIGRQLKSNLLNLNIEEEVREGLSELGINLDDLIE
                     AEVDPALGNGGLGRLAACFLDSMASLNISGQGYGIRYKYGLFEQKFVNGYQVEVPDNW
                     LTEGRYAWETVRPNEATMVKFGGEVELIKEGSHLKVIHKNYLPVMAMPYDIPIIGYQN
                     QCINTLRLFKSEIPKRDFGELTSNALNYSGSYEEALKHKYYTEEISQVLYPDDSNYAG
                     KLLRLKQEYFFVSAGIQDIIRKYKKNKLNINNLFDKVAIHINDTHPTLCIPELMRILL
                     DEENLSWDEAWEITKKTVSYTNHTIMSEAMEKWPVSMIKELLPRIYMIIEEINRRYVE
                     ELNNKGYEQDRIKRMSIIDCDNINMANLCIVTSHSVNGVAKLHTHILETEVLKDFYQD
                     EPNKFNNKTNGIAHRRWLISSNPQLSNLITDLIGDSWKKDTLQLKNIEKFKNDSSVLQ
                     RLDDIKYNNKANLAKFIKDKYDLNVDPSSIFDVQVKRLHAYKRQLLNIFNVLHMYHEL
                     LDNPNLNLDPRTFIFGAKAAPGYYLAKCIIKFINSVASTINNDVRVKDKLKVVFLENY
                     GVSLAEIIIPAANVSEQISTTTKEASGTSNMKFMMNGAITLATLDGANVEICEQVGKE
                     NMFLFGLSAEQVLNYNKYGGYSSLDLYHSNMDIKRVVDDLINGFIPNLGEEGRSIYNS
                     LTTYNDEYFVLRDFENYGQAQADINRLYRDKEKWNKMSLVNIANSGFFSSDRTISEYA
                     KDIWFKRV"
     gene            complement(5344..6786)
                     /gene="glgA"
                     /locus_tag="ACOIAF_003274"
     CDS             complement(5344..6786)
                     /gene="glgA"
                     /locus_tag="ACOIAF_003274"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HGZ2670269.1"
                     /translation="MKVFYVTAECWPFAKTGGLGDVSYALPKELKKEGVDVRVIMPKY
                     STIPSYLKDQLKEIAVFSVRVGWRNQYCGLLEMELDGVKFYFIDNEFYFRREDERKSI
                     YGYGDDAERYTFFTDAVLEAISRIDFYPDVIHINDWHTGMLPLILKERYATLEGYKNI
                     KTMYTIHNLQYQGVFDKHVLYDILDLPQKYFDNGDIEYYGSINFMKAGINFADKIITV
                     SPTYANEIQTSFYGEQLDGLLRKESGKLKGILNGIDYDLNDPAKDKDIFVHYDVDSID
                     KKVENKLRLQDILGLKKDSSIPLIGIVSRLVSQKGFDLIAYMMPELMREDLQIVVLGT
                     GEHQYQSMFNYYDSNFSDKVSARITFNASLAQQIYAASDMFLMPSLFEPCGIGQMLAM
                     RYGSLPIVRETGGLRDTVTPYNKFTGEGNGFSFKNYNAHEMFFCLKNAIKVFKDKEKW
                     IKLVENAMKTDNSWKKSAKEYIETYRDICD"
     gene            complement(6815..7945)
                     /gene="glgD"
                     /locus_tag="ACOIAF_003275"
     CDS             complement(6815..7945)
                     /gene="glgD"
                     /locus_tag="ACOIAF_003275"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HGZ2670270.1"
                     /translation="MRNECLGIINLNKKGDPAINKLNYGRPIASTPIAGRYRIIDFAL
                     SNMINSGITKVGIFAKEKYRSLTDHIGSGKDWDLSRKKGGLSIFSPENTKYRNTYSHR
                     EGDIYTILANLDYIEKSEEEYILIAPSYMLCNLNYSQALEYHKKSHNDITIIYKNVNN
                     ANKDFAGNLTLNLDSNNRVINVGNNLGKFPRANICMETYIMKREDFVECIYNIVNKGS
                     YCYLEEFIIEEAENMKIGAYEYTGYLKCVNSVESYFEMSKDLLNLEVADELLYSERKI
                     FTKEKNESPTIYTDSAKVENSFIASGCLIEGTVKDSIIFRKVNVEKGTVIENSIVMQN
                     CVIKSNAKLYNAILDKNTSVSNGKELRGDEKYPLVVEKNTNI"
     gene            complement(7947..9107)
                     /locus_tag="ACOIAF_003276"
     CDS             complement(7947..9107)
                     /locus_tag="ACOIAF_003276"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HGZ2670271.1"
                     /translation="MKKEMLAMILAGGQGSRLGVFTKRIAKPAVSFGGKYRIIDFVLS
                     NCSNSGIDTVGVLTQYRPLILNSHIGMGSHWDLDRINGGVYVLQPFMNEKEGNWYNGT
                     AHAIYQNMDFVDTYNPEYVLILSGDHIYKMDYSKMLKFHKEKGSKATIAVIEVPWDEA
                     SRFGIMNTNEDSSIYEFEEKPSEPKSNLASMGVYIFDWKMLRNYFKEAEKNPEINYDD
                     FGKNLIPKMLEDNVGMYAYPFKGYWRDVGTIQSLWDANMDIIKSPETLDLADPKWKIY
                     TNTMAMPPQYIGKNANVHRSMIADGCRILGEVGNSVLSHGVVVGKGSKVIDSVIMPNV
                     VIGENVTIEKAMIGECATINDNVQIKNVNNEINVVSEYENIEPRCVLIEGGL"
BASE COUNT         2939 a         1714 c          909 g         3762 t
ORIGIN      
        1 tttatatatg ttaatttaaa tacattattc tatcgtagtg ccttagagtt tttatttgta
       61 tttaaagtta aatttattct tgtttttcct ttaatattta atagtaaaac aacaaaatta
      121 taaaaaaaat aaagagaaac tttataaaag cttctctttt ttcatctctg ctataggtat
      181 cgttttccaa tgttctctca aaactctttc aacattttct aaatcattct ccctcataga
      241 ttttactact tcaatatgtt gattattgct gtatgtcaac attttatata actcatcttc
      301 attatcacta gtatatgact ttgacataaa attatatctt aaagagttca atagatttac
      361 taacacctga ttatctgatt tagttatgta tatatcgtga aattcattac ttaattttaa
      421 atagctattg taatttttat actttataga gaccgccatc atttctgtta attcttccat
      481 ttttactagg tctgattcag ttaaattttg taatgctgaa gaaccggcta acgcatccaa
      541 tactccaatt acggaatagg tgtctaattt ctccttttcc tgaaattctt ttactacaaa
      601 tcccttatta ggtatttttt ctagtaaatt ttcacctgct aacctagtta atgcttctct
      661 cgctggtgtt ctactaactc cgatagattt acaaatctca gattcagtaa ttctctgatt
      721 agcttcatat tctccatttt ggattttctt tgatatatag tcatatactt gagttgtaag
      781 tggatctacc ttcataagac gttcctccat attaagatta taaaagttaa atctccctac
      841 aactttggta gagagttcag ttttatctta ctaatttata taatatcaca attatgatat
      901 tttttatact tctttgtaat attttgaaat actaaaattc ttatgataat ttatacaaaa
      961 aaagtagaaa aagttttgat aaaacaaaac tctttccaca ttcaccttat tttctattta
     1021 tttttctaaa aaaagatatt taatttactc taaaaattct aaatgattta gagtctaaat
     1081 ctactttcaa ctcattattt tcaatttgta tagtactatt tgcattttta agactatact
     1141 ttataggtat ttcctcaata aaactactct ctatacataa tgatatactt tccacatttt
     1201 cacttctatt tactaaaatt aacattctat cattattttc attaactcta ataaaagaaa
     1261 atacagaatc attattagta tatataaatt ctgtatttcc agaacttaaa agtttattct
     1321 tatttctaat ttttataaca tctctataaa aattaagcat atcttcgtct tcatttttcc
     1381 atggataagt tcttctatta tctgggtcaa cacctccaca aagacctgct tcatctccat
     1441 aatatatata tggtactcct tcaaatgtca tctgtatagc tacagcaagc ttcactaaat
     1501 ctacatcatc atttaattcc gttttagctc ttgtaacatc atgtgttcca attaaattta
     1561 aattagacct aaatgattct tttggataat tttctttaat ctgcatatat ctattacaaa
     1621 gttcatatga attaatttct ccttttaaga aagaaaacat gttatttcta aaaggataac
     1681 ccataacaga atctaattct tcacctaata aataacttct tctctgacca tagctaatct
     1741 tatttgatgc atcttcccat acttcaccta taagaattga gtcatcatct gcctttttta
     1801 attccttttt taactctctt ataaacttag ttggtaattc gtctgcaaca tctagtctcc
     1861 agcccttaat acccatattt acccatttat ttataacgct atcattgtca tatattatat
     1921 aatccatata gctatgttct aattcattta tatttggcaa tgtcttaaca tcccaccaac
     1981 ttttatattt ttgaggaaat tcttcaaaca tataccatga ataatatgga gattctttag
     2041 attgatatgc ccctaagcta ttatagtttc catacatatt aaaatattta ctatctgctc
     2101 cagtatgact aaaaactcca tctaatacta ttgatatacc tttttctttt gctttatcaa
     2161 taagttcttt aaatgtatct tcatctccaa acattgggtc aattttttta tagtcacctg
     2221 tatcatactt atgattacta gaggcttcaa atatagggct tagatatagt atacttacac
     2281 ctaatttttt taaatatcca agtttattga ttatccctct taaatttcca ccatggaaat
     2341 cccatcttat aacatcccct tgactatcct ttatgtacat aggtgtatct tcccaatttc
     2401 catatatgaa actatttttt ttaggattat caaccttacc actacgattt ccattattga
     2461 atctatctac aaatatatga taaagtattc cttctttgta ccatgttggt actttaaaat
     2521 ctttataaac agtgatttga tatttattaa tatcactata attatattca caagaatatc
     2581 cattatctct gttttttcca taaaataaag tctttatctg accatccatt tcaacatcaa
     2641 ctttaaagta ataaaaataa acacctgtac catcaaactt ttcaatctta ccacaaaagc
     2701 actttccaag tttatcatta tctaattctt catttaaagt tattgttctc atttcttctt
     2761 ctctatttat ttctagggaa atagacttga tattgtatcc ctcatttact ttaacacaaa
     2821 tggtcaattc ctcatcaaat tttaaagctc caaacggagc cttaaaattt ttatcccaag
     2881 agttatattc tattacactt tgcatttaat cacacccttt taaaccaaat gtcttttgca
     2941 tactcagaaa ttgttctatc agaagaaaaa aatccagagt tagctatatt aactaaagac
     3001 attttattcc atttttcttt atctctatat aacctgttta tatctgcttg agcttgacca
     3061 taattttcaa aatctctcaa tacaaaatac tcatcattgt aagtagtgag tgaattataa
     3121 atacttcttc cttcttcgcc aagattagga ataaatccat ttattaaatc atctactacc
     3181 ctttttatat ccatattaga atgatatagg tcaagtgaag aatatccacc atatttatta
     3241 taatttagta cttgttctgc actaagtcca aataaaaaca tattttcttt accaacctgt
     3301 tcacatatct ctacatttgc accgtcaagt gtagccaaag ttatagcacc attcatcata
     3361 aacttcatat tacttgttcc agatgcttcc ttagttgtag ttgaaatttg ctcactaaca
     3421 tttgctgctg gtattattat ttctgctaat gatactccat aattctctaa aaatactact
     3481 tttaacttgt cctttactct aacatcatta tttatagtac ttgcaacaga atttataaat
     3541 ttaattatac atttagccaa ataatatcct ggtgctgctt ttgcgccaaa tataaaagtt
     3601 cttgggtcaa gattcaaatt aggattatca agtaattcat gatacatatg aagtacatta
     3661 aatatattca gtaactgtct tttatacgca tgcaatctct taacttgaac atcaaatatc
     3721 gaacttgggt ctacatttaa atcgtattta tcttttataa actttgctag atttgcttta
     3781 ttattgtatt ttatatcatc aagtctttgt aaaacagagg aatcattttt aaatttttca
     3841 atatttttta attgtaatgt atctttcttc catgaatcac caattaaatc tgtaattaaa
     3901 ttgctaagtt gaggatttga acttataagc catcgcctat gtgcaattcc attagtctta
     3961 ttattaaatt tatttggttc atcttgatag aaatctttta atacctctgt ttcaagaata
     4021 tgagtatgaa gcttagcaac tccatttaca ctatgacttg tgacaataca taagtttgcc
     4081 atattgatat tgtcacagtc tattatactc attcttttta ttctatcctg ctcatatcct
     4141 ttattattta attcctctac atatcttcta tttatttctt ctattatcat ataaattctt
     4201 ggtaatagtt ctttaatcat acttacaggc catttttcca ttgcttctga cattatagta
     4261 tgattagtat aggacactgt ttttttagtt atttcccatg cttcatccca agataaattt
     4321 tcttcatcaa gcaaaattct cataagttct ggtatgcata aggtaggatg tgtatcattt
     4381 atatgaattg ctaccttatc gaataaatta ttaatattta atttgttctt tttatatttt
     4441 cttattatat cttgtatccc tgcacttaca aaaaagtatt cttgtttcaa cctcaacaac
     4501 ttacctgcat aatttgaatc atcaggatat aatacctgtg atatttcctc agtataatac
     4561 ttatgtttta aagcctcttc atagctacca gaataattta gagcatttga agtaagttca
     4621 ccaaaatctc tctttggtat ttcactctta aacaatctta aagtatttat acattggttt
     4681 tggtatccga ttattggtat atcataaggc attgccatta caggaaggta gtttttatga
     4741 attactttta aatgactacc ttccttgatt aattctacct caccaccaaa cttcaccatt
     4801 gttgcttcat ttggtcttac agtttcccat gcatatcgtc cttctgtaag ccagttatca
     4861 ggcacttcta cctgataacc atttacaaat ttttgttcaa acagaccata tttatacctt
     4921 attccatatc cttgaccact tatgttcaaa gatgccatag aatctaaaaa gcaagctgct
     4981 aatctaccaa gtccaccatt tcctaaggct gggtctactt cagcttcaat taaatcatcc
     5041 aagttgattc ctaattcaga taaaccttct ctaacttctt cttcaatatt taaatttaat
     5101 aaattagatt ttaactgtct ccctattaaa aattctaaac taaaataata aacttctttt
     5161 ttcttatcta actttgtagc aacccatttt tttgctatct catctttcaa tagactacat
     5221 aatacattta acaattgttc atcagtagct tccttgatag attgtgcata taagctatac
     5281 atttttactt caaatttttg cttgaatttc tttttgctta ttgttaccaa aaaaaccact
     5341 cctttaatca catatgtctc tatatgtttc tatatactcc ttagctgatt ttttccaact
     5401 attatctgtt ttcattgcat tttcaactaa tttaatccat ttttctttgt ccttaaatac
     5461 tttgattgca tttttcaaac agaaaaacat ctcatgagca ttgtaatttt taaaactaaa
     5521 tccattgcct tctccagtaa atttattata aggtgttact gtatctctaa gccctccagt
     5581 ctctctaact attggtaaag aaccatatct catagctaac atttgaccta taccacaagg
     5641 ctcaaataat gatggcatta agaacatatc acttgcagca tatatctgtt gagccaatga
     5701 tgcattaaaa gtaattctag ctgatacttt atctgaaaaa ttagaatcgt aatagttaaa
     5761 cattgattga tattgatgtt ctccagttcc taaaacgaca atctgtaaat cttctctcat
     5821 aagttctggc atcatgtatg ctattaaatc aaatcctttt tgagaaacta atctcgaaac
     5881 tatacctatc aacggaatag atgaatcttt tttaagtccc aatatatctt gtaatctcaa
     5941 tttattttct acttttttgt caattgagtc tacatcataa tgaacaaata tatctttatc
     6001 ttttgcaggg tcatttaagt cataatctat accatttaat atacctttta attttcctga
     6061 ctcttttcta agtaatccat ctaattgttc accataaaaa gatgtttgta tttcatttgc
     6121 ataagtagga ctaacagtta taattttatc tgcaaaattt ataccagctt tcataaaatt
     6181 tattgagcca taatactcta tatctccatt atcaaagtat ttttgtggta agtccaatat
     6241 atcatataaa acatgcttat caaatacacc ttgatattgg agattatgta tagtatacat
     6301 agtttttata tttttatacc cctcaagggt tgcatatctt tcttttagaa ttaatggtaa
     6361 catccctgta tgccaatcat ttatatgtat tacatctgga taaaaatcaa ttctacttat
     6421 agcttctaaa actgcatctg taaagaaagt atatctttct gcatcatctc catatccata
     6481 gatactcttt ctttcatctt ctcttctaaa ataaaattca ttatctataa agtaaaattt
     6541 tacaccatct aattccattt ctaaaagacc acagtattga tttctccatc caacacgtac
     6601 actaaataca gctatttctt ttagttgatc ttttaaataa cttggtatag ttgaatattt
     6661 gggcattatt acccttacat ctacaccttc tttttttaat tctttaggca atgcataaga
     6721 aacatctcct aatcctccag tttttgcaaa gggccaacac tctgctgtta cataaaaaac
     6781 cttcattagt cattccccct tctaaaaaat ttatttatat atttgtattc ttttccacta
     6841 caagaggata tttttcatct cctcttaatt ctttcccatt tgacacactc gtatttttat
     6901 ctaatattgc attatatagt tttgcatttg actttattac acaattttgc attactattg
     6961 agttctctat aactgttcct ttttcaacat ttacctttct aaatatgata ctatctttta
     7021 cagtaccttc tattaaacaa ccagatgcta taaagctatt ttctacttta gcactatctg
     7081 tataaattgt aggtgattca ttcttttctt ttgtaaatat ctttctttca gaatataata
     7141 attcatctgc aacttctaaa tttaataaat ctttgctcat ttcaaaatat gattcaacag
     7201 aatttacaca ttttagatat cctgtgtatt cataggctcc tattttcata ttttctgctt
     7261 cttcaataat aaattcttct aaataacaat aactgccttt atttacaatg ttatatatac
     7321 attcaacaaa atcttcacgc ttcataatat atgtctccat acatatgttt gctctaggga
     7381 acttacctaa attatttcct acatttatta ccctattgtt actatctaaa tttagagtta
     7441 agttaccagc aaaatcttta tttgcattat ttacattttt atatattata gttatatcat
     7501 tatgagattt tttatggtat tccaatgctt gagaataatt taaattgcat agcatataac
     7561 ttggagctat taaaatatat tcttcttcac tcttttctat ataatctaga ttagctaaga
     7621 tagtatatat atcaccttct ctatgtgagt aagtatttct atacttagtg ttttctggac
     7681 taaaaattga taggccacct ttttttctac ttaaatccca atctttacca ctacctatat
     7741 ggtctgtcaa tgatctgtat ttttcttttg cgaatattcc aactttagtg attcctgaat
     7801 ttatcatatt agataaagca aagtctatta ttctatatct tccagctatt ggtgtagatg
     7861 ctattggtct tccatagtta agtttgttta ttgctgggtc tccttttttg tttaaattta
     7921 ttatccctaa acattcattt ctcatcttaa agacctcctt ctattaatac gcatctaggt
     7981 tcgatatttt catactcaga aactacattt atttcattat ttacattctt tatctgaaca
     8041 ttatcattta tagttgcaca ctcacctatc atagcttttt ctatagttac attttcacct
     8101 attacaacat ttggcattat tactgaatca attactttac ttccttttcc aacaacaact
     8161 ccatgtgata atacagagtt acctacttca cctaaaattc tacaaccatc agctatcatt
     8221 gatctatgaa catttgcatt tttacctata tattgtggag gcattgccat agtatttgta
     8281 tatatcttcc acttagggtc tgctaaatca agtgtttctg gagattttat tatatccata
     8341 ttagcatccc atagactttg tatagttcct acatctctcc agtatccttt aaatggataa
     8401 gcatacattc caacattatc ttctaacatt ttaggtatta aattttttcc aaaatcatca
     8461 taatttattt ctggattttt ttctgcttcc ttaaaataat ttctcaacat tttccaatca
     8521 aatatgtata ctcccataga tgccaaattg ctcttaggtt cacttggttt ttcttcaaat
     8581 tcatagatac ttgaatcttc atttgtattc attatcccaa atcttgaagc ttcatcccaa
     8641 ggaacttcaa tcactgctat tgtagcttta gagccttttt ccttatgaaa ttttaacatt
     8701 ttgctataat ccatcttgta tatatggtca ccagataata tcaatacata ttctggatta
     8761 taggtatcta caaagtccat attttgatat atcgcatgtg ctgtaccatt ataccaattt
     8821 ccttcttttt cattcataaa tggttgtaaa acatagacac caccatttat tctgtccaaa
     8881 tcccaatgac ttcccatgcc tatatgtgaa tttaatatca gcggtctata ttgagttaaa
     8941 acacctacag tatcaatacc agagtttgaa caattgctta acacaaaatc tataattcta
     9001 tacttacctc caaatgatac agctggtttg gcaattcttt ttgtaaaaac acctaatctt
     9061 gaaccttgcc ctcctgccaa aatcatagct aacatctctt ttttcattta atgacactcc
     9121 cctcatctag taattaatat ttataattgc atttttactt ttataaactt tatcagtcaa
     9181 taaatgtcgt gtataaatgt tgcttaaata ataccttaat aaaaataaaa taatatcaac
     9241 tatacaattg atataatgta taccccataa tggacttacc gacgtataca ttattttcca
     9301 taagcactat agtatttatt aata
//
LOCUS       DBGJFF010000142         9318 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.145, whole genome shotgun sequence.
ACCESSION   DBGJFF010000142 DBGJFF010000000
VERSION     DBGJFF010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9318
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.145"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            261..1055
                     /locus_tag="ACOIAF_003277"
     CDS             261..1055
                     /locus_tag="ACOIAF_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HGZ2670272.1"
                     /translation="MESVMENLNNLKVEVKDKVCVITINRPKALNALNSDTLRELSQV
                     IDVVSENEAILGVIITGEGKVFVAGADIRQMQNYKSEEGRKYAGYAQGIFDKIEALEK
                     TVIAAVNGYALGGGCELAMSCDIRIASEKAIFGQPEVNLGVIPCFGGTQRLSRLVGTG
                     IAKELIFTGRQVNAEEAKSIGLINKVVPSDLLLEESMKMMNQIVEKAPIAIRYAKVVI
                     NKGIDMDLKNALELEKDIAGLTFATRDKQEGMNAFIEKRKPVFENK"
     gene            1150..2490
                     /locus_tag="ACOIAF_003278"
     CDS             1150..2490
                     /locus_tag="ACOIAF_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HGZ2670273.1"
                     /translation="MNTGVIISLVGIIIALSLLVVMVMKGINIFIIAIVCSSLVAITG
                     SLNVYDALKVDYMTGFAGFLQANFFIFLTGTLMGKIMEITGGAKSIAKMIVRWIGKDK
                     ALLSIPIACGILAYGGVSVFVVSFAVFPIALEIFKEADLPRRFIPAALTFGCSTFAMV
                     APGAPQIQNIVPASTLGTDIMAGAVNGFISCAVMFIVGSTILYKMVSKEKANGGHFIA
                     HESDIFDEAEATNSKRENGPNGVIALIPLIVSILIINVKVNGKAIVPIEVGVFIGALL
                     VYLLLNKYQDNTKIVGHVGDACKTTVVAICNTCAVVAFGSVVKSAVGFDFIVNAMTNI
                     PGPPIAGAAVGTTVIAGICGSASGGLGIAAPLLGPVFLAKGVSAAALTRTMAISSAAL
                     DSLPHNGYIVTVTNGLCKETHKDAYMPIFWVTVLTPFIGTIVAVLLFTMFPGLP"
     gene            2627..3859
                     /locus_tag="ACOIAF_003279"
     CDS             2627..3859
                     /locus_tag="ACOIAF_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HGZ2670274.1"
                     /translation="MLNKPLEGVKVIDLTYFVAGPGTSKILADWGADVIKVEPSFGDP
                     GRKTGATMTMPIDDYNNPFYSTYNSNKRGLSINLKSETGIEIMDKLLSEANVFVSSYR
                     TGALKRLGLDYESLSKKHPHLIWGQINGFGDFGPAKDNAGFDTVAFWARSGAMLDIAE
                     KDTSPINPPIAFGDATTSCSLSGGICAALYQQAKTGKGQKVMASLFGQAIWNASSLLA
                     STQFSDEYPKTRKNAISPVINSYRCKDGKWIFLSILEHERYFEALCRLFGREDLIKEE
                     KFASSVESKKNATELINILDGEFAKFTQNEMVEKLTAADIAHEKIQHVNDVVTDEQAI
                     ANNYIYEHTSKNGNKTMLASTPVKFGNIEVNMTCDAPLIGEHSDEILRELGYSDSDIE
                     ALIESGIVTMNKEACEIC"
     gene            3899..5041
                     /gene="acrC"
                     /locus_tag="ACOIAF_003280"
     CDS             3899..5041
                     /gene="acrC"
                     /locus_tag="ACOIAF_003280"
                     /EC_number="1.3.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016628 - oxidoreductase activity, acting
                     on the CH-CH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0043958 - acryloyl-CoA reductase (NADH)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acryloyl-CoA reductase"
                     /protein_id="HGZ2670275.1"
                     /translation="MDFRLTEAQLMLQRVAKEFAENEIAPIAAETDKTGIFPRELFSK
                     MAKIGFNGIGTPVEYSGSGGADIEKVIVVTEIAKKCAASAAILSIHTIYAQAILKFGT
                     EEQKKKYLPMMAEGGCVGAFALTEPNAGSDAARAATTAIIDEETDEYVLNGTKCFISG
                     GGQAESLIIFALTDPSKGIKGMSAIIVDKGTPGFSIGKIEEKMGIHGSETAELIFDNC
                     RVPKSNLLGKEGKGFNIAMTCLDGARIGVGAQAVGIAEGALEESIKYSKERVQFGKPI
                     SALQGIQWYIADMATMVESAKLLVYYAADLKARGEKHTKEAAMAKYNASRTAREVTNL
                     ALQIHGGYGYMKDYPLERMYRDAKITEIYEGTSEIHKVVISRAVLG"
     gene            5052..5846
                     /locus_tag="ACOIAF_003281"
     CDS             5052..5846
                     /locus_tag="ACOIAF_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta"
                     /protein_id="HGZ2670276.1"
                     /translation="MNIVVCLKQVPDTNEVKINKETGTLIRDGVPSIINPDDRNALEE
                     ALKMKDELGAVIKVISMGPPQAKSALKEALAMGADEAYLISDRAFGGSDTWATSTIIA
                     AAIEKVGKYDVIFCGRQAIDGDTAQVGPEVAEFLGIPQVTYAKEVKVQDDKLLVTRYT
                     ETGDYLIEAKMPVLLTAIKELNNPRYPSVKGILEAYNNGDAKITVLTLADLDVDTTQI
                     GLKGSPTNVYKSFVPVKDKHNEIIEGINKKEKAEKLIEILFDLKLV"
     gene            5860..6879
                     /locus_tag="ACOIAF_003282"
     CDS             5860..6879
                     /locus_tag="ACOIAF_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HGZ2670277.1"
                     /translation="MRAKVNQGINLNDYNGVWVIGEQREGKINPVTIELIGEGRKLAD
                     QLGKELAVVIAGYEVEKEVKELLHYSVDKVYYINDPLLKDFTTDGYAISIANLIERKK
                     PEVVLVGATSIGRDIAPRIAGKVGTGLTADCTKLEIDSTDNKLLQTRPAFGGNLMATI
                     VCPKNRPQMSTVRPGVMAKAVRNESETGILEVVTPELTEKMIRTRLVEILPQEKKSVN
                     LTDARIIVSGGRGLKRAEGFELIKELADKLGAEIGASRAAVDSGWIEHSHQVGQTGTT
                     VRPELYIACGISGAIQHLAGMSDSKYIVAINKDAKAPIFSICDYGIVGDLYEIIPEMI
                     ESLNR"
     gene            6978..8354
                     /locus_tag="ACOIAF_003283"
     CDS             6978..8354
                     /locus_tag="ACOIAF_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 interaction domain-containing protein"
                     /protein_id="HGZ2670278.1"
                     /translation="MKEQWYKDIFARVLSMTDDGFIVVNTSGVIIDINDKYCDFLGKE
                     RKDIIGQNIQSIIPNTKMLDVMKNKYCEEGAIHHYSGGNTKEKSVIVSRSYVENDNGE
                     VVAGVAQVKFRLQSFDVAKKLMSEYMELQYYKEQFKDNCGFDKLIGENRDFIELKKTG
                     VKASKTNFPVLLTGETGTGKEVFARAIHNNSSRSDKPMVSINCAAIPEELLESELFGY
                     DEGAFTGAKKGGKKGKFLVANNGTIFLDEIGDMPLTMQAKLLRVLQESEIEPVGGLKT
                     IKIDVRVISATRKNLSKMVEEGLFREDLYYRLNVINIHMMELKDRQDDILLLANYILN
                     KLNVEYKELKVLSDKVKNCFINYTWPGNIRELQNVIKSAYAVSDDMVIMMCDLPSKMD
                     NISRVAQCNVDSNCSIHEMVENYEKSLIIDVLRKYNWKCSKAAEVMGIHKSLLYKKIK
                     KYEIELNN"
     gene            complement(8685..9029)
                     /locus_tag="ACOIAF_003284"
     CDS             complement(8685..9029)
                     /locus_tag="ACOIAF_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670279.1"
                     /translation="MDQIKRLHELQQKSYLTKEEFEELKNNTYIERFELRDECDNSYI
                     YTFYTNDKCNDIKFIKSQFIVTLIKIGFLCECGGVFRQKKIVDEYDYGYNAIYKCDSC
                     GKELDKEVENYD"
BASE COUNT         3472 a         1107 c         1864 g         2875 t
ORIGIN      
        1 tattttctcg aaaatgagac aaaaatatta ttttatgggt tttattgaag aaaaattctt
       61 ctgtgaatta caatttaatc aattttaaag ttgaaaattg ttaaaaattt agtgaaattt
      121 atgtatattt cgtttatttt attaaaaata acaatttttt actaaaaata aagaacttca
      181 atggtgattg gcatagtact tgctatttta tattaacaag aaatgtagaa aaaataacaa
      241 tgtataatgg ggggaatatg atggaatctg taatggaaaa tttgaacaat cttaaggtgg
      301 aggttaagga caaagtctgt gttattacta taaatagacc taaagcttta aatgctctta
      361 atagtgacac tcttagggaa ctatcacaag ttattgatgt agtatcagaa aatgaggcaa
      421 tattaggagt tattattact ggagaaggta aagtttttgt agccggtgct gatatacgcc
      481 agatgcaaaa ctataaaagt gaggaaggca gaaaatatgc aggttatgct cagggaattt
      541 ttgataaaat tgaagctctt gaaaaaacag taatagcagc agttaatgga tatgcattag
      601 gtggtggatg tgaacttgct atgagttgtg atattagaat tgcatcagaa aaggctatat
      661 ttggacaacc tgaagttaat ttaggagtta ttccatgttt tggtggaact caaagattat
      721 caagacttgt tggaactgga atagctaaag agttaatttt tacagggaga caagtaaatg
      781 cagaagaagc taaatctatt gggcttatta ataaagtggt tccaagtgat ttacttttag
      841 aagagtctat gaagatgatg aaccaaatag ttgaaaaagc gccaatagca ataagatatg
      901 caaaagttgt tattaacaaa gggatagata tggatttaaa gaatgctctt gaactagaga
      961 aagatattgc aggtttaact tttgctacaa gagataagca agaaggaatg aatgcattta
     1021 tagaaaagag aaagccagtt tttgaaaata agtagctatt ataactaaaa ttggaacaat
     1081 gtagaatata ttcaaaatct aaatttcgtg aaaaaataca tatagtttaa ttttaaaagg
     1141 agttaattta tgaatacggg agtaattata agtttagtag ggattatcat agctctatct
     1201 cttctagttg taatggttat gaagggaata aatattttta ttatagcaat tgtatgttca
     1261 tcattagttg ctataacagg aagtctaaat gtgtatgatg cacttaaagt ggattatatg
     1321 acagggtttg ctggatttct tcaggcaaat ttctttattt tcctaacagg gacattaatg
     1381 ggaaaaataa tggaaattac tggtggagca aaatctattg ctaagatgat tgtaagatgg
     1441 attgggaagg ataaagcact actttctatt ccaatagctt gtggaatatt agcctatggt
     1501 ggagtaagtg ttttcgttgt aagttttgca gtgtttccga tagctttaga aatttttaaa
     1561 gaagctgatt tgccacgaag atttatacca gcagctttga cttttggatg ctcaactttt
     1621 gctatggttg caccaggggc accacaaatt cagaatattg ttccagcatc aacattaggg
     1681 acagatatta tggctggagc agtaaatggt tttatatcat gtgcagtaat gtttatagtt
     1741 ggaagtacaa ttttgtacaa aatggtttca aaagaaaaag ccaatggtgg tcattttata
     1801 gcacatgaat cagatatatt tgatgaagca gaagcaacta attctaagag agaaaatggt
     1861 cccaatggtg taattgcctt aattccattg atagtgtcaa ttttaataat taatgtaaaa
     1921 gttaatggaa aagcgatagt accaattgag gtgggagtat ttattggagc tttattagta
     1981 tatttattgt taaataaata tcaagataat actaaaatag ttggacatgt tggtgatgca
     2041 tgtaaaacta cagtagtagc aatatgtaat acttgtgctg tagttgcatt tggttcagta
     2101 gtaaaatcag cagttggttt tgacttcata gttaatgcca tgactaatat tcctggtcct
     2161 cctattgcag gggctgcagt tggaactaca gttattgcag gtatttgtgg gtcagcttca
     2221 ggtggactag gaattgcagc tccacttctt ggaccagtat ttttagccaa gggtgtttcc
     2281 gctgcagcat taactaggac tatggcaatt tcatcagcag cattagattc acttccacac
     2341 aatggatata ttgttactgt aacaaatggt ctttgtaaag aaacacataa ggatgcctat
     2401 atgccaatat tctgggtaac tgttttaact ccgtttattg gaaccatagt agcagtccta
     2461 ctgtttacaa tgttcccggg attgccataa gagaaaagat aaaaggtata aattatattt
     2521 tgatattata aaatcaattt tcaaaatata tacaataaat ttcatgtttt aatactgtaa
     2581 atatgtattt aaaataacaa attgaccata taaggaggaa ttgaaagtgt taaataaacc
     2641 attagaggga gttaaggtta ttgacctaac gtattttgta gcaggtccag ggacttctaa
     2701 aatattagct gactggggtg ctgacgttat taaggtagag ccaagctttg gagacccagg
     2761 aagaaagaca ggggcaacaa tgacaatgcc aatagatgat tataataatc ctttttacag
     2821 cacatataat tcaaataaaa gaggtttgtc tataaatctt aagagtgaaa ctggaattga
     2881 aattatggat aagcttttaa gcgaagcaaa cgtatttgtt tcaagttata gaacaggagc
     2941 attaaaaaga ctaggtcttg attatgaatc attaagtaaa aagcatccac accttatctg
     3001 gggtcagata aatggttttg gcgattttgg acctgctaag gataatgcag gatttgatac
     3061 agttgccttt tgggcaagaa gtggggctat gcttgatata gcagaaaaag atacaagtcc
     3121 aataaatcca cctatagcct ttggagatgc aacaacttct tgtagtttat ctggaggaat
     3181 atgtgcagct ttgtaccagc aagctaagac tggaaaagga caaaaagtaa tggcatcttt
     3241 atttgggcaa gcaatatgga atgcatcatc tttattagca tctacacagt ttagtgatga
     3301 gtatccaaaa actagaaaaa atgctatttc accagtaata aattcttatc gttgtaagga
     3361 tggaaagtgg atattcttaa gtattttaga gcatgaaaga tattttgagg cactttgtag
     3421 attatttgga agagaagact taataaaaga agagaaattt gcatcttcag tagaaagtaa
     3481 gaaaaatgct acagaactta ttaatatttt ggatggtgaa tttgcaaagt ttactcaaaa
     3541 tgagatggtt gaaaaactta ctgctgctga tatagctcat gaaaaaattc aacatgttaa
     3601 tgatgtagtg actgatgaac aggctattgc taataactat atatatgaac atacaagtaa
     3661 aaatggaaat aagacaatgc ttgcatctac tccagttaaa tttggaaata ttgaagttaa
     3721 catgacttgt gatgctccac ttataggaga acattcagat gagatattga gagaacttgg
     3781 atacagtgat tctgatatag aggctctaat tgaatctgga attgtaacaa tgaataaaga
     3841 agcatgtgaa atatgttaat aaacatgtga agtatgttaa taaataggag ggaattttat
     3901 ggattttaga ttaacagaag cacaattaat gcttcaaaga gtagcaaaag aatttgcaga
     3961 gaatgaaata gcaccaatag ctgctgaaac agataaaaca ggtatttttc caagggagct
     4021 ttttagcaaa atggctaaaa taggatttaa tggaattgga acacctgtag agtatagtgg
     4081 ttctggtggg gcagatatag aaaaggtaat tgttgttact gagattgcca aaaaatgtgc
     4141 agcatctgca gcaatattat caatacacac aatatatgct caagctattt tgaaatttgg
     4201 tactgaggaa caaaagaaaa aataccttcc aatgatggct gaaggtgggt gtgtaggtgc
     4261 ttttgcctta accgaaccta atgctggttc agatgctgca agagctgcaa caactgcaat
     4321 tattgatgaa gaaactgatg aatatgtttt aaatggaact aagtgtttta tttctggtgg
     4381 aggtcaagca gaatcattaa taatatttgc actgacagac ccatcaaagg gaataaaggg
     4441 aatgtctgct atcattgtcg acaaaggtac tccaggtttt tcaataggaa aaattgaaga
     4501 aaaaatgggt attcatggtt cagaaactgc agagttaata tttgataact gtagagttcc
     4561 aaagtctaac ttattaggaa aagaaggcaa aggatttaat atagctatga cttgtttaga
     4621 tggagcaaga attggagttg gagctcaagc tgtaggaatt gctgaagggg ctttagaaga
     4681 aagtatcaaa tattctaaag aaagagtcca gtttggcaag ccaatttcag ctttgcaagg
     4741 tattcaatgg tatatagcag atatggcgac aatggtggaa tcagcaaaat tattagtata
     4801 ttatgctgca gatttgaaag ctagaggaga aaaacacact aaagaagctg ctatggcaaa
     4861 atacaatgcg tcacgtactg caagagaggt tacaaattta gcccttcaaa tccatggtgg
     4921 atatggatat atgaaggatt atccattaga gagaatgtac agagatgcca aaataacaga
     4981 aatttacgaa ggtacttcag aaatacacaa agttgttatt tcaagagcag tactaggata
     5041 ggaggaaaag aatgaatata gttgtttgct taaagcaagt tccagatact aatgaagtta
     5101 aaataaataa agagacaggt actctaataa gagatggtgt accaagtata atcaatccag
     5161 atgatagaaa tgctttagaa gaagctctaa aaatgaaaga tgaactagga gcagtgatta
     5221 aggttataag catgggacct cctcaagcta aaagtgcttt aaaagaagct ttggcaatgg
     5281 gagctgatga agcttattta atctcagata gagcttttgg tggttcagat acttgggcta
     5341 catctacaat tattgctgct gctatagaga aggttggaaa gtatgatgta atattctgcg
     5401 gaagacaagc tatagatgga gatacagctc aggttggacc agaggttgca gaatttttag
     5461 ggataccaca agttacttat gcgaaagaag taaaggttca agatgacaaa ttactagtaa
     5521 ctcgttatac agagacaggg gattatttaa tagaagcaaa gatgccagtt ttattaactg
     5581 ctataaaaga gttaaacaat ccaagatatc caagtgtcaa aggaatctta gaagcttata
     5641 ataatggaga tgcaaagatt actgtattaa cacttgcaga tttagatgtt gatacaactc
     5701 aaattggatt aaagggttca cctacaaacg tttataagtc ttttgtacca gttaaagaca
     5761 agcataatga aataatagaa ggtataaata aaaaagaaaa agctgagaaa ctaatagaaa
     5821 tactattcga tttgaaacta gtataggagt gttaagatga tgagagcaaa agttaatcaa
     5881 ggtataaatt taaatgatta taatggtgtt tgggtaatag gagagcaaag ggaaggtaaa
     5941 attaatcctg ttacgataga acttattggt gaaggaagaa agcttgctga ccaattagga
     6001 aaagagcttg cagttgtgat agctggatat gaagtagaga aagaagtgaa ggaattactt
     6061 cactatagtg ttgataaagt atattacatc aatgaccctc ttcttaaaga tttcactact
     6121 gatggatatg caatttcaat tgcaaattta atcgaaagaa aaaagccaga ggttgtattg
     6181 gttggtgcta cttctatagg aagagatata gcgcctagaa ttgcaggtaa ggttggaaca
     6241 ggtctaactg ctgactgtac aaaacttgaa atagattcaa cagataataa gttattacaa
     6301 acaagaccag catttggtgg aaatttaatg gccactattg tttgtccaaa aaatagacca
     6361 cagatgtcaa cagttcgtcc aggagttatg gcaaaagctg taagaaacga atcagagact
     6421 ggaattttag aagttgttac tccagaactt actgaaaaaa tgattagaac tagattagta
     6481 gagatacttc cacaagaaaa gaaatctgta aatctaactg atgctagaat aatagtatca
     6541 ggaggaagag gattaaaaag agcagaagga tttgagctta taaaagaatt ggctgataaa
     6601 ttgggggcag agattggtgc ttcaagagca gcagtagatt cagggtggat agaacattct
     6661 catcaagtgg gacaaacagg aactactgtt agaccagaac tatacattgc atgtggaata
     6721 tctggagcaa tacaacattt agcaggtatg agtgattcta aatatatagt tgctataaat
     6781 aaagatgcaa aagcccctat ttttagtata tgtgactatg gaatagtagg agatttatat
     6841 gaaattatac ctgaaatgat agaatcatta aataggtaac ttttagaaaa cattgaatat
     6901 gtagaatgat aagtcttata atattgtatt gtaagactta ttatttttgc tgaaggttat
     6961 agtggggaga tgaaacaatg aaagagcagt ggtataagga tatatttgca agagtattat
     7021 ctatgactga tgatggtttt attgttgtaa atacaagtgg agtaataata gatattaatg
     7081 ataaatattg tgacttttta ggaaaagaaa gaaaagatat aataggacaa aatatacaaa
     7141 gtattatacc aaatacaaaa atgctagatg ttatgaaaaa taagtattgt gaagaaggag
     7201 ctatacatca ttattcaggt ggaaatacaa aagaaaaaag tgtaatagta agtaggtctt
     7261 atgtggaaaa tgacaatgga gaagtagttg caggagtagc tcaagttaaa tttagattgc
     7321 aatcatttga tgtagctaaa aagctaatgt cagaatatat ggagctccaa tattataaag
     7381 agcagtttaa ggataactgt ggttttgaca agctcatagg tgaaaataga gacttcatag
     7441 aacttaagaa aacaggtgtg aaagcttcta aaacaaactt tccagtactt ttaacgggtg
     7501 aaacaggcac aggtaaagaa gtttttgcta gagcaattca caataatagc agtaggtcag
     7561 ataaacctat ggttagtata aattgcgctg caataccaga agaattatta gagtcagagc
     7621 tatttggcta tgacgaagga gcttttacag gagcaaaaaa gggtggtaaa aaaggtaaat
     7681 ttttagttgc gaataatggt acaatctttt tagacgagat aggtgacatg cctttgacca
     7741 tgcaagcaaa acttttaaga gttcttcagg aaagtgagat agaaccagtg ggaggtctta
     7801 aaactataaa gatagatgta agagtaatct ctgccacaag aaagaattta agtaaaatgg
     7861 ttgaagaggg attatttaga gaagacctat actatagatt aaatgtaata aacattcata
     7921 tgatggaatt gaaggacagg caagatgata ttttattgct agcaaattat atattaaaca
     7981 aattaaatgt tgaatacaag gaattgaagg ttttaagtga taaggttaaa aattgtttta
     8041 ttaactatac ttggccggga aatataagag aactacaaaa tgtaataaaa agtgcctatg
     8101 ctgtaagtga tgatatggtc attatgatgt gtgaccttcc atctaagatg gataatatca
     8161 gcagagtagc tcaatgtaat gtggatagca attgttcaat tcatgaaatg gttgagaatt
     8221 atgaaaagag cttaattatt gatgtgttaa ggaagtataa ttggaaatgt tctaaggcag
     8281 cagaggttat gggaatacac aagagtcttt tatataagaa aataaaaaag tatgaaattg
     8341 aattaaataa ttaagataac ttgaattaag gcaatgctaa aaaacgccct tattcaaagc
     8401 gaactgtata aactgtttta tgcagttaaa acgtgtaaaa gtagatgtga agtactaatg
     8461 tcaatcatta gtactttttt attatattta taattactat ctatatttca gtatcttttg
     8521 atatctaaga ttattatgtt taatttagga tataacaaaa ccctcttagt tatggacagt
     8581 tttttatatt ttaactgtct actaagatgg gatagtttag tttaaattta gagtaaattt
     8641 tatctagcat ttataaaaaa tattttgaat agcttttttg ttatttagtc ataattttct
     8701 acttctttat caagttcttt gccacaagaa tcacacttat atatagcatt gtatccgtaa
     8761 tcatattcat caactatttt cttttgtcta aatacaccac cacactcaca taggaaacca
     8821 atttttataa gtgtaacgat aaattgtgat tttataaatt ttatatcatt acatttatcg
     8881 ttagtgtaaa atgtataaat ataagagtta tcgcactcat ctcttaattc aaatctttca
     8941 atatatgtgt tattttttaa ttcctcaaat tcttctttag ttaaatatga tttttgttgc
     9001 aattcatgta aacgcttaat ttggtccatt atttatatcc tcccaataat tttagtattt
     9061 taagcttaga taaggaatct aagttttatt aaatttatcc caaggaatta tttaaatcat
     9121 atttagtttt attaattaca acatatataa attacacgaa aataaaacta attgtaaaaa
     9181 taatatcctg tgaaaaccta aactactaac cccttactaa cccgaaaact aaaaaaagaa
     9241 aatatatcca aatataagtg atatataaat aaagatgttt catataaatt taactttaaa
     9301 tattctgaca ataatata
//
LOCUS       DBGJFF010000143         9295 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.146, whole genome shotgun sequence.
ACCESSION   DBGJFF010000143 DBGJFF010000000
VERSION     DBGJFF010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9295
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.146"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(70..402)
                     /locus_tag="ACOIAF_003285"
     CDS             complement(70..402)
                     /locus_tag="ACOIAF_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HGZ2670280.1"
                     /translation="MNTREMKEYRGFYNKKLSETDEFFRVFGSLDDKTYSEGAISKKN
                     KELMGLAISILSRCDECICYHIEGCINSGASKDEIIEAIKIGVIGGGSITYPNARFAI
                     KILEEFNI"
     gene            complement(646..1287)
                     /locus_tag="ACOIAF_003286"
     CDS             complement(646..1287)
                     /locus_tag="ACOIAF_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HGZ2670281.1"
                     /translation="MKKAINKQFILSTLICFIPFIVSIYFYNRLPNEVAIHFDNYGNP
                     DNYAPKVIAAFGVPLLMLCIHLYTWFRLENEPKKANFSNVIQNLSKWGVAILSVIIQI
                     MMVLYSTGVSLESNFYTGLILGIIVILFGNYLPKCKRNYSVGIILPWTLNDEDNWNKT
                     HHLAGWIWLIGGILLIINAFINIPFYNIFVIFVIVILPFIYSYLMYKISGKNN"
     gene            complement(1268..1561)
                     /locus_tag="ACOIAF_003287"
     CDS             complement(1268..1561)
                     /locus_tag="ACOIAF_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autorepressor SdpR family transcription factor"
                     /protein_id="HGZ2670282.1"
                     /translation="MGFPETFKALSDPIRREILVMLRENKMSAGEISEKFNMTNATIS
                     YHLSQLKKAGLIFETKYKNFIFYEINISVFEEVMLWFSQFMEKGEKDNEKSDK"
     gene            1942..2004
                     /locus_tag="ACOIAF_003288"
                     /pseudo
     CDS             1942..2004
                     /locus_tag="ACOIAF_003288"
                     /inference="COORDINATES: protein motif:HMM:NF024729.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2185..2319)
                     /locus_tag="ACOIAF_003289"
     CDS             complement(2185..2319)
                     /locus_tag="ACOIAF_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670283.1"
                     /translation="MNISRRAMKIIELAQKIANKRGVTVQDAWNDAMKEYKEKYEYVA
                     "
     gene            complement(2530..3282)
                     /locus_tag="ACOIAF_003290"
     CDS             complement(2530..3282)
                     /locus_tag="ACOIAF_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HGZ2670284.1"
                     /translation="MFSVIFLCIGGFLAAFVDSIVGGGGLISMPVLMAIGVPVHLAIG
                     TNKFAASAGCISSAYRYAKSGKINNDLLKKLVPFTIIGSVLGVRCVLSISEEILNVLV
                     VVMILIVAIYTFISKNLGQEDNFEAVNKKNLRLGMLMALIMGFYDGFFGPGTGTFLTF
                     GFIKIYGYDFLHASANTKILNLTSNITSLLLFMINGQVDYKIAIVFALVMIMGAYVGA
                     KVAIKKGSKMIKPIFLVMALFMVVKLVYQTLV"
     gene            complement(3341..4996)
                     /locus_tag="ACOIAF_003291"
     CDS             complement(3341..4996)
                     /locus_tag="ACOIAF_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGZ2670285.1"
                     /translation="MVDFIESNNEVQKFDWGEVMWIHEPSKTQFNRLSAGIVRFFPGN
                     CQEKHFHLSEEQLLYVIQGEGIQIIDGKKVNIKETSIVYCPPYSEHEIINTGKIDLVI
                     LITYVPHKFSSLRQIPIVFSENNIQELVNVNIIENLANQISNILKLRISVYDLKYKEI
                     FETKKENKFCDICKNIKQCTKKLVKSINNDNFEKVYHCEYGVSSLEIPIVLEENIVGY
                     IECGNFIVYKSIDIDKNLSELSSSSGIDIKYINKIYNEFPLNPKSRLYVLKENLLMMS
                     EFIQEIAKRNFFENQLSIKDEEILKSRKENIRLEEALKKANSRLLEEEYIINPIDMFN
                     NSSKEYPFELELYIEKEIKNMNLEGVYNLIDTNKLRYNDVRDTIQEMIFVLSRTVLRD
                     LEDLKLISYLRNKYNKKISLVNSDEDLWNVLFEFSKECIDKNRAFWRKDKGNLIENIN
                     EYIKKYYKENINLNSISDVFFISPNYLSSIFNERNKVSITEYINLLRIEESKKYLLDR
                     SMSISDICKKVGFNNSSYFSQIFKKFNSITPNEYRKNMLDNKY"
     gene            complement(6269..7348)
                     /locus_tag="ACOIAF_003292"
     CDS             complement(6269..7348)
                     /locus_tag="ACOIAF_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGZ2670286.1"
                     /translation="MILITLCILVAVFVLAFAGLRLIAPTIIRTSHKIEAPGIDRMEM
                     VEIGGIQQALYIRGQNADNPIILWVHGGPGNSMMPFLHLYQYEWENDFTVVNWDQRNV
                     GKTYFANDPAAVLQTMSAERVLQDVHEVTAYLKQEFNKEKIILIGHSWGTVLGTMAVQ
                     TYPEDYSAYIGIAQTVNMVDNERVGYEKTLEMVKAAGKQKDIKALEAIAPYPPAKYGP
                     SYLAALMTVRTYQSKYGLSINMDLGNIISILATPYYSLRELKNTAIVNPNAIDNQGDV
                     LRFLVEEADIRNYGVDYQVPVYYIMGENDYNSPYLPAKKFFDEIVAPDKQIFFIPDAD
                     HLPFLSKKAEFDCVLLEEIAPKLAK"
     gene            complement(7432..8088)
                     /locus_tag="ACOIAF_003293"
     CDS             complement(7432..8088)
                     /locus_tag="ACOIAF_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HGZ2670287.1"
                     /translation="MTRKNNPKQAIENIITISAKLFAEKGYDKTSMQNIVDASGMSKG
                     GIFHHFSSKEDIFNVVMERRFEQIIETVNQWLGEMHGLTAKEKLRSLVRRHLTDEVIK
                     ESSNMITSAIESPHIILAFTQDNLKKLAPILANVLREGIEDRSIITEFPDECAEVILL
                     LFNFWCDTDVFQGDFPTLRKRFQFLQFLMRQIGVDILEDEIIESINIFYERKPMGQVE
                     "
     gene            8410..8616
                     /locus_tag="ACOIAF_003294"
     CDS             8410..8616
                     /locus_tag="ACOIAF_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670288.1"
                     /translation="MISHAAEICKKYKMPFIIFPTIEKKSIEEWVNIGANMLCFAQDT
                     TFITYVINQLLSFYNEIVPIQKQH"
     gene            complement(8769..9197)
                     /locus_tag="ACOIAF_003295"
     CDS             complement(8769..9197)
                     /locus_tag="ACOIAF_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HGZ2670289.1"
                     /translation="MSKIYIVYWSGTGNTEKMANFVAEGVKLKGKTPEVLDVSLLKPS
                     DLKEEDKFALGCPSMGAEQLEEGYMEPFVSELESMVSGKQIGLFGSYGWGNCEWMRDW
                     EERMQNAGATIIGGEGITAMEDPNEEAKDECIELGKTLAE"
BASE COUNT         3105 a         1658 c          996 g         3536 t
ORIGIN      
        1 aataaagctt aatttcaaag gcttaaatag actattatta ttagtctaaa taaactacgt
       61 gtatcaaaat tatatattaa attcttctaa aatttttatt gcaaatcttg cgtttggata
      121 tgtaatagac cctccaccaa ttacacctat ttttatagct tctataattt catctttact
      181 tgctccagaa ttaatacaac cctcaatatg ataacaaata cactcatcac atcggcttaa
      241 aattgaaata gctagtccca ttaattcctt atttttttta cttatagccc cttctgaata
      301 agtcttatca tcaaggcttc caaatactct aaaaaactca tctgtttcac ttaatttttt
      361 attgtaaaat cccctatatt ctttcatttc tctagtattc atcttctacc tcctattttt
      421 ttcttataag agtatcacac tgtacttata ttttcaatat taaatacaaa ttttcatttt
      481 atgttcatta gcctaattta tattattttt tgaaatctca ataagttatc atataagtaa
      541 gattttttca gaactaaaat agaaatctta gtaatttctt tattacctat ctcagttctg
      601 aaatatatta ttcttatcca aacattatta tattgattaa ttatattagt tatttttacc
      661 tgaaatttta tacatcaaat aagaatatat aaatggtagt attacaatta caaagattac
      721 aaagatatta taaaaaggaa tattaataaa tgcattaata attaataata ttccaccaat
      781 aagccaaatc catccagcta aatggtgtgt cttattccaa ttatcttcat cattcagagt
      841 ccatggcaaa attattccta ctgagtaatt tcgtttacat ttaggaagat agtttccaaa
      901 taaaataact attattccta aaattaatcc tgtataaaaa ttagactcca aactaactcc
      961 tgttgaatat aaaaccatca ttatttgtat aattactgaa agtatagcaa ctccccattt
     1021 tgataaattt tgtataacat ttgaaaaatt agctttctta ggctcattct caagtctaaa
     1081 ccatgtatac aaatggatac atagcattaa caatggtaca ccaaaagctg ctataacctt
     1141 tggagcataa ttatctggat ttccataatt atcaaaatga attgcaactt catttggtaa
     1201 tctattataa aaatatattg atacaataaa tggtataaaa catattaaag tagataaaat
     1261 aaattgttta tttatcgctt ttttcattat ctttttctcc cttctccata aattgtgaaa
     1321 accaaagcat aacctcttcg aaaactgaaa tatttatttc gtagaaaata aagttcttat
     1381 actttgtttc aaatatgagt cctgcctttt ttagttgaga tagatgataa gaaattgtag
     1441 cattcgtcat attaaatttt tcactaattt ctcctgctga cattttattc tccctcaaca
     1501 taaccaatat ttctcttctt atagggtctg aaagagcttt gaatgtttct ggaaacccca
     1561 acaccatcac ctcgctattt agaatttcat ctaaatagaa tataacataa catccatttt
     1621 atttcaatag ctatttagaa atttttctaa atagttattt ctaaatattt attgaaaatt
     1681 ttcatattaa gattctcaat ttacttaagt cgcttttatt taatcttact ttatctaatt
     1741 tttttaactt aacaaaagtg tattgcacca taattgttaa agcaaaaacc taatgactga
     1801 ggtacttata tatataacaa tacttttaat tttaaattaa aagtagctaa acccaattta
     1861 aaaagtagtt aagtcctatt taaatagaaa agaatgaaac gagaaatata ctgtataaaa
     1921 gtacataaaa acatctacta attagataca actataacag aatatattta ttattataat
     1981 aacaaaagaa taaaaaaaaa ttaatttagc ataaccttgt ttaatataga tttgaatatg
     2041 aatttaaagt tatgtaaaat taaaaataca ggaatgaaaa ttccattcct gtataaaatc
     2101 taacttttga gtctcagtac aatcttattt tgagacaatc ttttttgttt tatattttct
     2161 tatatttaaa atataacgca tatcttatgc aacatattca tatttttctt tatattcttt
     2221 catcgcatca ttccaagcgt cttgtactgt tacacctctt ttattagcaa ttttttgtgc
     2281 cagttcaatt atcttcatag ctcttcttga tatgttcata aacattcctc ctacaaatta
     2341 ccatatattt catatatata aatcaaggtt atatttttat gatatataat ccttctattg
     2401 taagattaca gtttatatgt gtattatata tgaactatat tagaagataa ttagaatttt
     2461 tataaagcta tatgattaga ttaaatataa aagctttaca attacttata aatttaaaaa
     2521 gtctataagc taaactaatg tttgatatac caattttacg accataaata atgccattac
     2581 taaaaatatt ggttttatca tctttgaacc tttctttata gcaactttag cacctacata
     2641 agctcccatt atcatcacta atgcaaacac tatagcaatt ttataatcta cttgaccatt
     2701 tatcatgaat aataacaatg aagttatatt acttgttaag tttaatattt ttgtatttgc
     2761 agaagcatgt aaaaaatcat atccatatat ttttataaat ccaaaggtca aaaaagttcc
     2821 tgtacctgga ccaaaaaaac catcataaaa acccattata agagccataa gcatccctag
     2881 tcttaaattt ttcttattaa cagcttcaaa attatcttct tgacctaaat ttttagaaat
     2941 aaaggtgtaa atagccacta ttaaaatcat aaccacaacc aaaacattta atatctcttc
     3001 acttattgat aatacacatc tcacacctag aacactacca ataatagtaa atggtactaa
     3061 tttttttaaa aggtcgttat taatttttcc agactttgca tatctatagg cactacttat
     3121 acatcctgct gatgctgcaa atttatttgt accaatggca agatgtactg gcactccaat
     3181 tgccataaga acaggcatgc ttataagtcc tcctcctcct actatagaat ctacaaaagc
     3241 agctaaaaac ccccctatac ataaaaaaat aactgaaaac ataaaattcc tccatcattc
     3301 tctttcaatt tttcttacaa tatatattat ataatcttat ttaatactta ttatccagca
     3361 tattctttct atattcattt ggagttatac tattaaactt cttaaatatt tgtgaaaaat
     3421 aactactgtt attaaaacct actttcttac aaatatcact aatactcatg cttctatcta
     3481 acagatattt tttagattct tctatcctaa gaagatttat atactcagtt attgaaactt
     3541 tatttctttc attaaaaata gaactcaaat aatttggact tataaaaaaa acatctgata
     3601 tactattcaa atttatattt tctttataat atttttttat atattcgttt atattttcaa
     3661 ttagatttcc tttatctttt ctccaaaatg ctctattctt atcaatacac tctttggaaa
     3721 attcaaataa tacattccat aaatcttcat cactgtttac aagtgatatt tttttattat
     3781 atttatttct taaataagat ataagtttta aatcctctaa atctctaagc actgttctag
     3841 acaatacaaa tatcatttcc tgaatagtat ctctaacatc attatatctt aatttgtttg
     3901 tatctattag attataaaca ccttctaaat tcatattttt aatttctttt tcaatataaa
     3961 gttctaattc aaagggatat tcttttgaag aattattgaa catatctatc ggattaataa
     4021 tatactcttc ttctaataat ctactatttg cttttttaag tgcctcttca agtcttatat
     4081 tttcttttct actcttcaat atctcctcat cttttatact aagttgattt tcaaaaaaat
     4141 tcctcttagc tatttcctga ataaattcag acatcattaa taaattttct tttaatacat
     4201 ataacctact tttaggattt aatggaaatt cattgtaaat tttgtttatg tattttatat
     4261 caataccaga actacttgat aattcagata aatttttatc tatatcaata gacttatata
     4321 ctataaaatt accacattct atatatccaa ctatattttc ttctaatact ataggtatct
     4381 ctaaactgga aacaccatac tcacaatggt acactttttc aaagttatca ttatttatgc
     4441 ttttaacaag ttttttagta cactgtttta tatttttaca aatatcgcaa aatttatttt
     4501 ctttcttagt ctcaaatatt tctttatact ttaagtcata tacagatatt ctaagcttca
     4561 atatattaga tatttgattt gcaagatttt caattatgtt tacattaact aactcctgta
     4621 tattgttttc agaaaatact ataggtattt gtctcaaact cgaaaattta tgaggaacat
     4681 aagtaattaa tattactaaa tcaattttac cagtgtttat aatttcatgt tctgaatatg
     4741 gtgggcaata aactatagat gtttccttta tattgacctt tttaccatca attatttgta
     4801 ttccttctcc ctgaatcaca tataaaagct gttcttcact taaatgaaaa tgcttttctt
     4861 ggcagtttcc cggaaaaaat cttacaattc cagcactaag tctattgaat tgtgtttttg
     4921 atggttcgtg tatccacata acctctcccc aatcaaattt ttgtacttca ttatttgact
     4981 caataaaatc caccataaac tcacctcgta gtttttgtct aaatattttt tcttcccttc
     5041 ttatattaaa ataatatcat catcattaac tttcattact ataaaatcat catactcttt
     5101 taatactcct gtaattttag ttgcacttag tttactacaa ttcagttttt taatatattg
     5161 ataaattcta taattacttt tttattttgt aaaatcagtg ttttcccaaa ataattatat
     5221 ctataaatga aagtattttt actgcttaca tcaatatatg ttatcataat tttccttttt
     5281 attgcatata taaataactg tatataacat tgtccaatac tatatagaaa tacatctaag
     5341 aaagaatatt attaaattgt tccaattagt attatttttc ttataaaaat aaaaaaactg
     5401 ttggattctt ttagaaaaat tattttttac ataccttttt tgcttagata gtctatcttc
     5461 tcagctattt tgtatataca ctcttctagt tcatgatttt attttaatat cttgcttata
     5521 ttgctgaaat aattgaattc ttttatctat catattgtct cttatatcat aaacatattg
     5581 tttcttatat cacaaaaatg aaagcattct tatatcttca atatataaac ctgctttttt
     5641 taatcttgta ataaatatag gatactctat attctcttcc tagaaataac taataccact
     5701 tatttttcta ttatatagaa agtaataaat ctttttctta ataaaattca atagtaagtg
     5761 gaataaaatt ttatatgaat aagaacatta taaaaatact ttatacaata gtcaataaaa
     5821 gatattagct tcaaattatg ttaaagttgt attatttaaa gtgaaaaaac aaataaaaaa
     5881 gaaataatag tataagtaga aaaatatact taaaacaagg cgaagcctct cttgtatcta
     5941 aatcaagtag agaattaaat ctaaaaaaga aaataaatat aaaagatgac tcctgtacat
     6001 tacaagaatc atcttatggt ttcataatca ctccatggtt tttaactgtc tgatataaag
     6061 gtctcagttc actcatagca tgaccaccct ttccgattgc tgaccccgtc acttatggac
     6121 aaaatgtcca taagtgatag tcctctctta aaaccctcta aatagatata tctcaagcac
     6181 ctcattgaaa ccagttgctg gaaacaaact gaagcgttct ggaatagtaa aagaaattgc
     6241 caccatatcc acgttactgt cctagttgtt actttgctaa cttcggagca atttcctcta
     6301 acaatacgca gtcaaattct gcctttttgc taagaaatgg cagatggtct gcatctggta
     6361 taaaaaaaat ctgtttgtct ggcgccacta tttcatcaaa aaatttcttc gctggaagat
     6421 aggggctatt atagtcattt tctcccatga tgtagtatac agggacctga tagtcaactc
     6481 cataatttct tatgtcagct tcttccacta aaaaccgcaa tacatcacct tgattgtcaa
     6541 tcgcatttgg attgacaatg gccgtatttt ttaactctct caatgaataa tacggggttg
     6601 ccagtatact gattatattg cccaaatcca tatttataga aagcccatac ttgctttggt
     6661 atgttcttac tgtcataaga gctgccaaat agcttggacc atattttgct ggcggatatg
     6721 gtgcaatagc ttccagcgcc ttgatatctt tttgcttccc tgcagctttc accatttcaa
     6781 gtgtcttttc atagcccacg cgctcattgt ccaccatatt aacggtctgc gcaatcccaa
     6841 tgtacgcgct gtaatcctcc ggataggttt gcacagccat agttcccagc actgttcccc
     6901 agctatgacc tataaggata attttctcct tgttgaactc ttgcttcaaa tatgctgtca
     6961 cctcatggac gtcttgcaga acacgctccg cgctcatggt ctgcaaaact gctgccggat
     7021 cgttcgcaaa gtatgttttg cctacgtttc gctgatccca gttcaccaca gtaaaatcat
     7081 tttcccactc atactggtac agatgtaaaa atggcatcat tgaattacca ggacccccat
     7141 gaacccaaag gatgatgggg ttgtctgcgt tttgtccccg gatatataac gcctgctgta
     7201 tgccgccgat ctccaccatt tccatgcggt cgattccagg agcttctatt ttatggcttg
     7261 tacgtataat cgtgggggct atcagacgca aaccggcaaa cgccagaaca aaaacagcaa
     7321 ctagtatgca aagtgtgatt aatatcattt taagtacctt ctttcatgaa tttttttagc
     7381 catagatatc tcctctaaca ttaaaaaaaa tatgttgcta ttttagtttt gttactctac
     7441 ctgtcccatt ggtttccttt cataaaaaat attaattgat tctataatct catcttccag
     7501 gatatcaact cctatttgtc tcataagaaa ctgtaagaat tgaaaccgtt tacgaagtgt
     7561 aggaaagtcc ccctgaaata catctgtatc acaccaaaag ttaaagagca gcaaaattac
     7621 ctctgcgcat tcatctggga actctgtgat gatgcttcta tcctctatgc cttctcgtaa
     7681 aacatttgct aatattggtg ctagtttttt caaattatcc tgtgtaaatg ccagtatgat
     7741 atgcggactt tctatagcgc ttgtaatcat gttactagat tcttttatca cctcatccgt
     7801 taaatgccgc ctaactaaac ttctaagctt ctcttttgct gtaagtccat gcatttcacc
     7861 gagccattga tttactgtct ctattatctg ttcaaagcgc ctttccatga ccacattaaa
     7921 aatatcttct tttgaactga agtgatgaaa aattccgccc tttgacattc ccgaagcatc
     7981 aacaatattt tgcatacttg ttttgtcata tcctttttca gcaaacaatt ttgcagaaat
     8041 ggtaatgata ttttcaatag cttgctttgg attgttcttt ctagtcatta tataactcct
     8101 tcttattaaa aaccgactgt tggtttttat tatataagct attcctactt ctgtcaagca
     8161 acattatgtt tatggcaaaa caataaggtt tggtggttag acaggtatta attagactaa
     8221 tgcgactttc ccctagatgg aacccttggt aaatttgcaa tctttaccgg ctttgattat
     8281 atctgtaagc aaggcaggta tggccacacc tgccctgttt gaccctcgga tattcattcc
     8341 tatccctcct ctccttagcc attacaccaa aacgccgctc attgtctcca atggcggcgt
     8401 tttaaagata tgatttctca tgctgctgaa atatgcaaaa aatataagat gccctttatt
     8461 atttttccta caatagaaaa gaaaagtatt gaagaatggg ttaatattgg agcaaatatg
     8521 ctttgttttg cacaagatac tacctttatc acttatgtta tcaatcaatt attatctttt
     8581 tataatgaga ttgtaccaat tcaaaaacaa cattaaatta taaatctaat tttatattaa
     8641 aagagactaa tattttatct atctctatta aggatatttt cttatgtatc ataatattag
     8701 tctctcttat tattatatac tgctatcatc attagttgct atttatacta tttttataat
     8761 atacaaattt attcagctaa cgttttgcct aattctatac actcatcttt tgcttcttca
     8821 tttgggtctt ccattgctgt aattccttct ccaccaataa ttgtagcacc agcattttgc
     8881 atacgttctt cccagtctct catccattca caatttcccc atccatatga accaaataat
     8941 ccaatctgtt tacctgatac catagattct aattctgaaa caaatggctc catatacccc
     9001 tcttctagtt gctctgctcc catagatggg caacctaatg caaatttatc ttcttctttt
     9061 aaatcacttg gtttcagtaa gctcacatct aaaacttctg gtgtcttacc ttttaacttc
     9121 acaccttcag ccacaaaatt tgccattttt tcagtatttc ctgttccact ccaataaaca
     9181 atatatattt tactcatttt tacctcctgt agtatttttt aattaatcaa tagttagtta
     9241 tagctaacta acatctaaaa aacaaaagat ttctttatct gaaatctctt tattt
//
LOCUS       DBGJFF010000144         9241 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.147, whole genome shotgun sequence.
ACCESSION   DBGJFF010000144 DBGJFF010000000
VERSION     DBGJFF010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9241
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.147"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            92..1180
                     /locus_tag="ACOIAF_003296"
     CDS             92..1180
                     /locus_tag="ACOIAF_003296"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HGZ2670290.1"
                     /translation="MRDFSTYSKDDVIFLLKDISNLIEEEGNKERELKIQNGRHYSEM
                     IPIEYEVSEDYLNLYHEKLRENKDKLAFSIGVMCEKIIKKNGNDVVLVSLARAGTPIG
                     ILAKRYIRSKYNISLPHYTISIIRDKGIDMNAIEYIVKNHPSSKIQFLDGWTGKGTIS
                     KELEKACDELNVKFKKYFDSTLAVLADPAGYSGLYGIREDFLIPSACLNSTVSGLVSR
                     TVLREDLIGKNDFHGAKFYRHLKDKDESMNYIETIEECFKNQFKNISDEVENWESDII
                     TRDGYFDVLNIKEKYNITDINFIKPGVGETTRVLLRRVPYKILVKDLNDKSLDHIFIL
                     AKEKNVEVEQMDLKAYKCCGIIKNMKDI"
     gene            1208..1999
                     /locus_tag="ACOIAF_003297"
     CDS             1208..1999
                     /locus_tag="ACOIAF_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HGZ2670291.1"
                     /translation="MIVFSDLDRSIIYSNKFLNADSKYTNIEIYREKEISYISLDTIN
                     LIKQIQYYGMFIPTTTRTVEQFKRIEFNKYGIYFPWSITSNGGVILKDNEILKSWSEK
                     IDKLKSDYEPIESMIHKFKDYLNVDGITNFKVAEDTFFYIVVDLSRFNLDSIKEYTNI
                     LESKNWKFYVSGRKIYFIPKEISKENAIKYLTKELGIEYFYAVGDSIMDYGMLNISNK
                     SYVLKHGDINKNEIENSFISSSFNGMSGTEEILSNILDENCLLNI"
     gene            complement(2114..2668)
                     /locus_tag="ACOIAF_003298"
     CDS             complement(2114..2668)
                     /locus_tag="ACOIAF_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HGZ2670292.1"
                     /translation="MNIDVGSKIRSLRKSKNISISTLAKNSDLSTGLISQIERNMVVP
                     SIVAMWKISKALDINIGYFFEEIGREDSDIVVKKNNRKKIVTNDSTKFYELLVPNLSG
                     KKIEFILVTLDGHTQKNSEFVTHEGEECGYIIKGKMKITLGNKEYLLEEGDSFYFNST
                     IPHVYENYDDEVCISVWAMTPPSF"
     gene            2864..4066
                     /locus_tag="ACOIAF_003299"
     CDS             2864..4066
                     /locus_tag="ACOIAF_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HGZ2670293.1"
                     /translation="MKSFVSPDNIWVLWAVVTGWAAFSIYLEQKYNWASKVSGAIIAL
                     VGAMLLSNLNIIPVESVVYDQVWGYVVPLAIALLLYQCNIKKIWKESGRLLIIFLVGS
                     VGTVLGAMIGFLALKNVVPDLNIVAAMMTGSYIGGNVNFAAMSGAFDAPGELVSATVV
                     ADNLLMALYFFVLIAIPSIGFFRKHFKHPHVDEMESIGITEGETVAANYWGRKEISLK
                     DIAIAIGSAFIIVAVSVELSTWFKAIIPLSNPFLAMLNTLFGNQYLIITTLTMLFATF
                     MPNFFGDIKGAQELGTFLIYIFFVVVGVPASITLIIQKSPLLLLYCGIMVLINMLVTF
                     IVAKIFKFSLEEAILASNANIGGPTTAAAMAISKGWSKLVGPILIVGTFGYIVGNYFG
                     LLVGNILI"
     gene            4142..5122
                     /locus_tag="ACOIAF_003300"
     CDS             4142..5122
                     /locus_tag="ACOIAF_003300"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HGZ2670294.1"
                     /translation="MIFDGHSDTWADVTIRRLNGEKDVFRRIHLDNFKRANIEGAIFV
                     IWIDPPYDKEPEKRVKQIISSIKDEIEDMKDVVNIVKKYSDIDKGISENKINILIGLE
                     GLSHIGRNIDLLDYFYKEVGARHASLTWNEQNELATGCLGDENRGLTEIGKKAVNKME
                     KLGMIVDVSHLNEKSFWDLMSVASKPVIASHSNARSLCDAKRNLTDNQLKEISNTGGL
                     VGINSFRGFTNSNPKKQDLIHLVDHIDYMVNLIGIEHVSFGFDFSDYLGKETIGSFTG
                     NASVPSVDGLESTHTAQNIVVELENRGYKKKDIELISSGNFYRLFKDILK"
     gene            5365..6318
                     /locus_tag="ACOIAF_003301"
     CDS             5365..6318
                     /locus_tag="ACOIAF_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HGZ2670295.1"
                     /translation="MKKRYLVIGIIILSYFSLFIGAEDINIMHIFAKNQHKLMIFIMS
                     RIPRLISILIAGVGMSIAGLIMQQISKNKFVSPTTGATIDAAQFGIVICMLLVPTASI
                     FTKTIIAFVFSLVGTFTFMKIIGKLQFKNIIFVPLVGIMFGNIIGSMTDFIAYKYDLS
                     QNVSSWMQGDFSMILKGNYEILYITIPLIILAYIYANKFTVVGMGMDFATNLGLSYKR
                     IVNIGLIIVALVTVCVVVTAGNIPFIGLIVPNIVSLYMGDNIRANIWYTGLLGAIFVL
                     ICDIFGRIIIYPYEISIGLTVGVIGSILFLYLILRRNVNEA"
     gene            6308..7264
                     /locus_tag="ACOIAF_003302"
     CDS             6308..7264
                     /locus_tag="ACOIAF_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HGZ2670296.1"
                     /translation="MKLSLNQKIYFVSILLFISIALFIIYGIDMNHLEYALSQRIPKI
                     FAMVLGGGCIAFTTVVFQTITNNQILTPSVLGLDSLYVMIQTIIVFIFGSSSSLIINE
                     NYNFIINVAMMIGASLLLYKMLFEKNKNNIFFLILVGMIFGTLFKSATTFIQVMIDPN
                     EFLALQTSIMASLNNINTNVLLIAFIIIIAIIPFIYDEIKYLDVLSLGKEQAINLGVD
                     FDKAVKKMIILIAILVSISTALIGPMTFLGLLLANITREIFKTYKHTYLISGSMLIGM
                     ITLIIGQFFIQHVFKFDTTLSVVINFIGGIYFIQLLLKGANR"
     gene            7261..8016
                     /locus_tag="ACOIAF_003303"
     CDS             7261..8016
                     /locus_tag="ACOIAF_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2670297.1"
                     /translation="MIEIKNIFKRYKNKNVVDDVSFSIEKGKITSFIGPNGAGKSTVL
                     SIVTRLIGGDGGEVIIEGKSLTNYSNKELAKKIAILKQSNNITLKLTIRELVGFGRFP
                     YSEGNLTKEDENYIDEAIEYMKLTDIQHKYLDELSGGQRQRAYIAMVIAQDTEYILLD
                     EPLNNLDMNHSVQMMKVLRSLCDELDKTIVLVMHDINFASCYSDNIVALKNGKVEKVG
                     RTDEIVNEKVLEDIYEMNFNIKNINGNRICIYF"
     gene            8108..9073
                     /locus_tag="ACOIAF_003304"
     CDS             8108..9073
                     /locus_tag="ACOIAF_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HGZ2670298.1"
                     /translation="MNKKAAIVAAVAIIGLVTVFALGGSKKNESKTSENSNNTIKITH
                     NLGETDVKLNPKKVVVFDYSALDTMDALGVAENLVGLPKASLPASLEKYKDDKYADLG
                     GLKEPDLEGIKSANPDLIIINGRQEDFYEQLSKIAPTISTSKDDKKYLESVKNNIDKI
                     AKIFGVEEKANQEFSKIEKKIETLNKKVTDKNLNALTIMVNEGNLSVFGEESRFSILY
                     NSFGFENKDKNIKESSHGQNITFEYIAKQNPEVMFVIDRGIATGSDVKESSTAKSVLN
                     NDIIKSMDAYKNDNIIYLDSPTWYVNDGGLTSLNKMIDDASKAVN"
BASE COUNT         3638 a          935 c         1543 g         3125 t
ORIGIN      
        1 caagtataaa agaattaact atagtatgtt gtagctaaag tgttttaaca ggataaaata
       61 tatcacgatt ttaagatagg agttgagtca tatgagagat tttagcacgt attctaaaga
      121 tgatgttatt tttttattaa aagatatatc aaatttaatt gaagaagaag gaaataaaga
      181 aagagaatta aaaatacaaa atggtagaca ttattcagaa atgataccaa tagagtatga
      241 agttagtgaa gattatctga atttatatca tgagaaacta agagaaaata aagataaact
      301 tgcattttct ataggagtta tgtgtgaaaa aataattaaa aaaaatggaa atgatgtagt
      361 actagtttca cttgctagag caggaacacc tattggtatt ttggcaaaaa gatatataag
      421 aagcaagtat aatataagtt tacctcacta tacaatctct ataataagag ataagggaat
      481 agacatgaat gcaattgaat atatagtaaa gaatcatccg agctcaaaaa ttcaattttt
      541 agatggatgg actggtaagg gaactatatc aaaagaactt gaaaaagcat gtgatgaact
      601 taatgttaaa tttaaaaaat attttgactc aacacttgct gtattagctg accctgctgg
      661 atattcagga ctttatggaa ttagagaaga tttcttaata ccaagtgcat gtttaaattc
      721 aaccgtttct ggtttagtaa gtagaactgt actaagagag gatttaattg gtaaaaatga
      781 ctttcatgga gctaagtttt atagacattt aaaagataaa gatgaatcta tgaactatat
      841 cgaaactata gaagaatgtt ttaaaaatca atttaagaat atatctgatg aagttgaaaa
      901 ctgggaaagt gacattataa caagagatgg atactttgat gttttaaata taaaagaaaa
      961 gtataacata acagatatta actttatcaa accaggtgta ggggaaacga caagggtttt
     1021 acttagaaga gttccgtata agattcttgt taaagactta aatgataaat ctttagacca
     1081 tatattcata ttagctaagg agaaaaatgt tgaagttgaa caaatggatt taaaagccta
     1141 taaatgttgt ggaataataa aaaatatgaa agatatttag gaaatatatt agacaggaga
     1201 atattgaatg attgtttttt cagatttaga caggagtatc atttactcca ataaattttt
     1261 aaatgctgat agtaaatata ctaatataga aatttatagg gaaaaagaaa tatcttatat
     1321 atctttagat actataaatt taataaagca aatacaatat tatggtatgt ttatacctac
     1381 aactactaga acagtggagc aatttaagcg tatagaattt aataaatatg ggatttattt
     1441 tccttggagt ataacttcaa atggaggagt tatacttaaa gataatgaaa tattgaaatc
     1501 atggagtgaa aaaatagata aactaaaatc tgattatgag cccattgaaa gtatgattca
     1561 taaatttaaa gactatttga atgtagatgg aataactaat tttaaagttg cagaagatac
     1621 atttttttat atagttgttg atttatcaag atttaatttg gattcaatca aagaatatac
     1681 aaatatattg gaatcaaaaa attggaagtt ttatgtgagt ggcagaaaaa tatattttat
     1741 accaaaagaa atttcaaaag aaaatgctat aaaatacttg actaaagaat taggaataga
     1801 atatttttat gcagttggag attctattat ggactatggt atgcttaata tctcaaataa
     1861 atcctatgta ttgaagcatg gtgatataaa taaaaatgaa atagaaaatt catttatatc
     1921 aagttctttc aatggaatga gtggaactga agagatacta tctaacattt tagacgagaa
     1981 ctgcctactt aacatataat gactgatata taaataataa actagaaaaa tctgaaataa
     2041 attatagtaa agtttatcat actaatttat ttcagatttt tatcaattta tatttaagta
     2101 agaactcaat atgttaaaat gaaggtggag tcattgccca gactgagata catacctcat
     2161 catcataatt ttcatacaca tgtggtatag tactgttaaa ataaaaacta tctccttcct
     2221 ccaatagata ttccttattt ccaagtgtta tcttcatttt accttttatt atatagccac
     2281 attcttctcc ttcgtgtgtc acaaattcac tgtttttttg tgtatgacca tctagcgtaa
     2341 caagtataaa ttctattttt ttcccagata aattaggaac aagaagttca taaaattttg
     2401 tagaatcatt agttactatt ttttttcgat tatttttctt tactactata tcagagtctt
     2461 ctcttccaat ctcttcaaaa aaataaccta tatttatatc aagagctttt gatattttcc
     2521 acattgctac aatagatgga acaaccatat tacgctcaat ttgacttatt aagcctgtac
     2581 ttaaatctga attttttgct aaagtagaga tacttatatt tttagatttt ctaagagatc
     2641 taattttgct tccaacatct atattcatta gatacaaacc ccctcagaca tgattataac
     2701 atataattta taaagattaa taaaataaaa aaaaagtttt acaatacaag tattttttat
     2761 gctatattga attttctgaa aagatgcatt ataatagtat taatattcaa tatattgaat
     2821 attgataacg atattttaat ttaatgaaat ggaggtaatt aagatgaaga gttttgtaag
     2881 tcctgataat atttgggtat tatgggcagt tgtaactgga tgggcagcat ttagtattta
     2941 tttggaacaa aagtacaatt gggcatcaaa agtgtcggga gcaataatag ctcttgtagg
     3001 agcaatgtta ttatcaaatc taaatattat accagtagag tctgttgtat atgaccaagt
     3061 atggggatat gttgttcctt tagccatagc tttattgtta tatcaatgta acattaaaaa
     3121 gatatggaaa gaaagtggaa gattgctcat tatatttctt gtaggttctg taggaactgt
     3181 acttggagct atgattggat ttttggcatt aaaaaatgta gtaccagatt taaatattgt
     3241 tgcagctatg atgacaggtt cttatatagg tggaaatgta aactttgctg ctatgtcagg
     3301 cgcgtttgat gcgccaggag aattagtatc agcaacagtt gtagcagata atcttttaat
     3361 ggcattatat ttctttgtct taatagctat accatcaatt ggatttttta gaaaacattt
     3421 taaacatcca catgtagatg aaatggaaag tataggtata actgaaggag aaacagtagc
     3481 agcaaattat tggggaagaa aagaaatatc tttaaaagat atagcaatag ccattggtag
     3541 tgcttttata attgtcgcag tatctgtaga actatcaact tggtttaaag ctataatacc
     3601 attatcaaat ccttttttag caatgttaaa tactttattt ggaaatcaat atctaataat
     3661 aactactttg actatgttgt ttgccacatt tatgcctaat ttctttggtg atataaaagg
     3721 agcacaggaa ttgggtacat ttttaatata tatattcttt gttgtagtag gtgttcctgc
     3781 ttcaataaca ttaataattc aaaaatcacc attactactt ttatactgcg gaataatggt
     3841 actaataaac atgctagtta catttatagt tgcaaaaata tttaagttta gtttagaaga
     3901 agctattctt gcatctaatg caaatattgg tggtcctaca acagcagcag ctatggcaat
     3961 atcaaaagga tggtctaagc tagtagggcc aatacttata gttggaacat ttggatacat
     4021 agtcggtaat tattttggtc ttttagtagg aaatattttg atataatttt acgtgaaaaa
     4081 aagttgtttt ttagaaagat ttttatttat tagaaagatt tttatttaat tggaggttgt
     4141 tatgattttc gatggacatt cagatacttg ggcagatgta acaataagaa ggcttaacgg
     4201 ggaaaaagat gtatttagaa gaatccatct tgataatttt aaaagagcaa atatagaagg
     4261 agctattttt gtaatatgga tagacccacc atatgataaa gagcctgaaa aaagagttaa
     4321 acaaattata agctctataa aagatgaaat tgaagatatg aaagatgttg taaacatagt
     4381 aaaaaaatat tcagatatag ataaaggtat aagtgaaaac aaaataaata ttttaattgg
     4441 tcttgaagga ttaagccata ttggaagaaa tatagattta ctagattatt tttataaaga
     4501 agttggtgca agacatgcat cattaacttg gaatgaacag aatgaacttg ctacaggatg
     4561 tttaggggat gaaaatcgtg gtttaactga aataggtaaa aaagcagtca ataaaatgga
     4621 aaaattgggc atgatagtag atgtgtcaca tttaaatgaa aaatcatttt gggatttaat
     4681 gtctgttgca agtaagccag ttatagcttc tcattctaat gctaggagtt tatgtgatgc
     4741 taaaagaaat cttacagata atcaattaaa agaaatttca aatacaggtg gattagttgg
     4801 aataaatagt tttagaggat ttactaatag caatcctaag aagcaagatt taatacattt
     4861 ggttgaccat atagactata tggtaaatct tataggtata gaacatgtat cttttggttt
     4921 tgattttagt gattacttag ggaaagagac tataggttct tttactggaa atgctagtgt
     4981 gccaagtgta gatggtcttg aaagcacaca tacagcacag aacatagtag tagagcttga
     5041 aaatagaggt tataaaaaga aagatataga gttaatctct agtggtaatt tttataggct
     5101 ctttaaagat atactaaaat aatataataa agaacttaaa tttataaaat agtatattta
     5161 tacaaataat atctacatat ttaaattggc gttcataatt tattaaattt taatgttact
     5221 agaagtttca attctagtaa cattttttat gttaaaaaca gatatagata tgtattgaca
     5281 atgataatta ttattgataa actataaaag tgaaaatgat aataattttc attttaatta
     5341 ataaaagatt tttaaggagg gagaatgaag aaaaggtatt tagtcatagg aataataatt
     5401 ttgtcttatt tttctttatt tataggagct gaagatataa atataatgca tatatttgcc
     5461 aaaaatcagc ataaacttat gatatttatc atgagtagaa taccaagatt aataagtata
     5521 ttgattgctg gtgtaggaat gagcatagca ggtcttataa tgcaacagat aagtaagaat
     5581 aaatttgtat cacctacaac aggtgctaca atagatgcag cacaatttgg gatagtaata
     5641 tgtatgcttt tagtaccaac tgcctctatt tttacaaaga caataatagc atttgtattt
     5701 tctcttgtag ggacttttac gtttatgaaa ataataggga agttacaatt taaaaatata
     5761 atctttgtac cattagtagg tataatgttt ggaaatataa taggttctat gacggatttt
     5821 atcgcgtata aatacgattt gagtcaaaat gttagttctt ggatgcaagg tgacttttct
     5881 atgatactta aaggaaatta tgagatatta tatatcacaa taccccttat aatattagca
     5941 tatatttatg caaataaatt tactgttgtt ggtatgggta tggattttgc gacaaactta
     6001 ggtcttagtt acaaaagaat tgtcaatata ggtctgataa tagtagcact tgttacagta
     6061 tgtgtagtcg ttacagctgg taatatccct tttataggtc taatagttcc aaatatcgta
     6121 tccttgtata tgggtgataa tataagagca aacatatggt atactggttt acttggagcg
     6181 atatttgtac ttatatgtga tatttttgga agaattatta tttatccata tgaaatatct
     6241 atagggctta cagttggagt tataggaagt atactattcc tttatttaat attaaggagg
     6301 aatgtaaatg aagcttagtc tcaatcaaaa aatttacttt gtaagtatat tactattcat
     6361 atcaatagca ctttttataa tttatggaat agacatgaat catttagaat atgcattatc
     6421 acaaagaata ccaaaaatat ttgctatggt attaggtgga ggatgtatag catttactac
     6481 tgtagtgttt caaacaatta caaacaatca aatattaaca ccaagtgtac taggtttaga
     6541 ttcattgtat gtaatgattc aaactataat agtattcata tttggttcaa gcagtagtct
     6601 gattataaat gaaaattata attttattat taatgtagct atgatgattg gagcttcatt
     6661 gttattatat aagatgttat ttgaaaaaaa taaaaataat attttctttt taatcctagt
     6721 aggaatgata tttggaacct tatttaaaag tgcaacaaca tttattcagg taatgataga
     6781 cccgaatgaa tttttagctt tacaaactag tataatggca agtttaaaca atataaacac
     6841 taatgtattg ttaatagcat ttataataat tattgccata atacctttta tatatgatga
     6901 aataaagtat ttagatgtat tgtcgttagg taaagaacaa gcaattaatt taggtgtaga
     6961 ttttgataaa gcagttaaaa aaatgataat attaatagca attttagtct caatatcaac
     7021 agcacttatt ggacctatga catttttagg acttttactt gctaatataa caagagaaat
     7081 ttttaaaaca tataagcata catacttgat ttcgggttct atgttgatag ggatgattac
     7141 attgataata ggtcaatttt ttatacaaca tgtatttaag tttgatacaa ctttaagtgt
     7201 agtaataaat tttataggag gaatttactt tattcaatta ctattaaagg gggcaaatag
     7261 atgatagaga taaaaaatat atttaaaaga tataagaata aaaatgttgt agatgatgtc
     7321 tcttttagta tagaaaaggg aaagataaca tcttttatag gaccaaatgg agctggaaaa
     7381 agtacagtac tatcaatagt tacaagactt ataggaggtg atggtggaga agtaataata
     7441 gaaggaaaaa gcttgactaa ttatagcaat aaagaattag caaaaaaaat agctattctt
     7501 aaacaatcta acaatataac tttaaagctt acaataagag aattagttgg atttggaaga
     7561 tttccatata gtgaaggtaa tctaactaaa gaggatgaaa attatattga tgaagcaata
     7621 gaatatatga agttgactga tatacaacat aagtatttag atgaattgag tggaggtcaa
     7681 agacaaagag cttatatagc aatggtaata gctcaagata cagagtacat attacttgat
     7741 gaaccactga acaatttgga tatgaaccat tccgtacaga tgatgaaggt acttagaagt
     7801 ctttgtgatg agttagataa gactatagta ttagtaatgc atgatataaa ttttgcatca
     7861 tgttattcag ataatatagt tgcattaaaa aatggaaaag ttgaaaaagt aggtcgtaca
     7921 gatgagatag taaatgaaaa agttttagaa gatatttatg agatgaattt taacattaag
     7981 aatataaatg gaaatcgaat ctgtatttat ttttaaaata ttgtatatga aaataaactt
     8041 gctatataat tttttataaa gtttttaaat taaaataaag attaatagga gagaggaaaa
     8101 tattgatatg aataaaaaag cagcaatagt agcagcagta gctattatag gattagtgac
     8161 agtgtttgca ttaggtggaa gtaaaaagaa tgaatcaaaa acatcagaaa attcaaataa
     8221 taccataaag ataactcata acttgggaga aacagatgta aaattaaatc ctaaaaaagt
     8281 agtagtattt gattattcgg ctttagatac aatggatgca ttaggtgtag ctgagaattt
     8341 agtaggactt ccaaaagcga gtttaccagc atctttagaa aaatataagg atgacaagta
     8401 tgcagattta ggaggattaa aagaaccaga tttagaaggt ataaagtcgg caaatccaga
     8461 tttaattata ataaatggaa gacaagaaga tttttatgag caattgtcaa aaatagcacc
     8521 aacaataagt acaagtaaag atgataagaa gtacttagaa tctgttaaaa ataatataga
     8581 caaaatagca aaaatatttg gagtagaaga aaaagcaaat caagaattta gtaaaattga
     8641 aaagaaaata gaaactttga ataaaaaagt aacagataaa aatttaaatg ctttaactat
     8701 aatggtaaat gaaggtaatt taagtgtatt tggtgaagaa tcaagattta gtatattata
     8761 caatagtttt ggatttgaaa ataaagataa aaatataaaa gaatcaagtc atggacaaaa
     8821 tattactttt gaatatatag ctaaacaaaa tccagaagtt atgttcgtta tagatagagg
     8881 aatagctact ggcagtgatg taaaagaaag ctcaactgca aaatctgttt tgaacaatga
     8941 tataataaaa tctatggatg catataagaa tgataatata atatatttag attcaccaac
     9001 atggtatgta aatgatggtg gtctaacatc tttaaataaa atgatagatg atgcttcaaa
     9061 agcggtaaat taataatccg atataattaa tcaaaatgta ttttttataa aatgattatc
     9121 aaataaacta ggagttttta agaactccta gtttattttt tgcataaaaa agatatagtt
     9181 taaacaataa tattatctat aataagataa atctattcaa aatctaataa aatatgaagt
     9241 t
//
LOCUS       DBGJFF010000145         9105 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.148, whole genome shotgun sequence.
ACCESSION   DBGJFF010000145 DBGJFF010000000
VERSION     DBGJFF010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9105
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.148"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(190..924)
                     /locus_tag="ACOIAF_003305"
     CDS             complement(190..924)
                     /locus_tag="ACOIAF_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine biosynthesis TtcA family
                     protein"
                     /protein_id="HGZ2670299.1"
                     /translation="MSIQKLLSKARKAIQDFDMIQENDKIAVGLSGGKDSLTLLHILK
                     SYQRFSPQNFELIAITLNTGGVDNSPLDKLCKEINVPFYEFQTDIKEIVFDIRQEKNP
                     CALCANLRRGALNDNAKKLGCNKVALGHHKDDAIETFLMSMFYEGRVNCFSPKTYLDR
                     QDLTVIRPMVYIEEYMTKKISKDSNYPIITNPCPANGHTRREYIKNLIANLNKEMPDF
                     KRNVFGALNNSEKLFIWDKEKIKIFK"
     gene            1049..1765
                     /locus_tag="ACOIAF_003306"
     CDS             1049..1765
                     /locus_tag="ACOIAF_003306"
                     /EC_number="3.4.17.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HGZ2670300.1"
                     /translation="MKKFKLIGAIILVVFISVFISKDRLLKKDNDPIVAAQHDSEQDV
                     EANSQNKNHSNSISNILLVNKTNGISKNYTPENITKVNIPFVEEATEEEKQMAGEPAK
                     AVEDLVKQANSEGIQFLGSSAYRSYDTQLDTYTRRVKSQGREKADVYVAKPGYSEHQT
                     GLCIDLTNPERWFVGSTKEAKWLAENAHKFGFIIRYPEGKEDITGTAYEPWHIRYVGK
                     DAAEEIYSKGLTLEEYLQNK"
     gene            1899..2591
                     /locus_tag="ACOIAF_003307"
     CDS             1899..2591
                     /locus_tag="ACOIAF_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGZ2670301.1"
                     /translation="MNNTVLVVDDEKEIRDLIEIYLLNSGYNVIKAEDGKEALDILKK
                     ENIHLMILDIMMPKMDGIEVCRKVRESLNIPILMLSAKSEDMDKIQGIMTGADDYLTK
                     PFNPLELTVRVKSLIRRAYYFSGANNEDKDVIKVGVVIIDKEKHSVTVAGNEIILTSR
                     EFDILYLLANNKGRVYSTEEIFEKVWKEKYYQSNNTVMVHMSRIRDKIEKYTDGEKII
                     HTVWGVGYKIEK"
     gene            2581..3762
                     /locus_tag="ACOIAF_003308"
     CDS             2581..3762
                     /locus_tag="ACOIAF_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGZ2670302.1"
                     /translation="MKNKSLLNHMFRPILDLIISCALTSMLILTSMYIIKSLYVNSDM
                     SVVNQLVYQARDFRSLIGYDLFYPFLFTITSLAIYSIISYKRNKQIVDFIRKTNRKNQ
                     KLEIIKSAVNLMDEGNLEKSIDIEYDLDILKDKEKDDIDELAHKINNIVHQLRNITIE
                     ERQAQQTKTDLITNVSHDLRTPLTSIMGYLGVIEEGKYKDEVQMMYYVDIAYEKSKNL
                     NVLINDLFELTKMQNNTIKLNKIEINLVELLSQVVSQFEIYFKQEFMVSRINFNEEKL
                     IVKADPNKLVRAFENLITNAVKYGKDGHYVDIVTEKKEDMAVVKVINYGEPIPVLDLP
                     NIFDRFYRVEKSRNRNVGGSGLGLAITKNIVNLHDGEITVSSDKYQTVFEVQLPIIQS
                     A"
     gene            4044..4499
                     /locus_tag="ACOIAF_003309"
     CDS             4044..4499
                     /locus_tag="ACOIAF_003309"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HGZ2670303.1"
                     /translation="MEKVESFKLDHTKVKAPFVRKCSVLDGVKGDKVTKFDLRFLQPN
                     VESFGTAAMHGLEHLLATYLRDTLDGVIDLSPMGCRTGFYLILWGDVDAKTVKIGLEE
                     ALKKVLESDKMPAATAIECGNYRDLSLFGAKEYAKDVLDKGFSLNIYGE"
     gene            4489..5214
                     /locus_tag="ACOIAF_003310"
     CDS             4489..5214
                     /locus_tag="ACOIAF_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HGZ2670304.1"
                     /translation="MENNLIGREDISINQSEVLRYLQYRGQEIDKDLKYTIDECVAIT
                     KNKINPRYLSRIYPIKINKDNVVELEGTNLTLESKDVYELLKECNECVIMAATIGIDI
                     EKEIRRHSYSNLTKGLIIDSCGTTAIEEVCDIVQNNLEKVFLKQDKFITMRYSPGYGD
                     LPIEKNIDILNVLNAQKQIGLTITSNGIMIPRKSVVAIIGISNTRINKSKKEKSCENC
                     KNYRNCTYRKGANSCGNKTISEK"
     gene            5186..7567
                     /locus_tag="ACOIAF_003311"
     CDS             5186..7567
                     /locus_tag="ACOIAF_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HGZ2670305.1"
                     /translation="MEIRQYLKNNILIFDGAMGTMLQQKGLKLGENPEVFGLQNPDKL
                     IEIHTAYLEAGSNVILTNTFGCNELKLDSKYTVEEVIDNAVLVARKAIENVDNTKPRY
                     VALDIGPIGEMLEPMGTLSFDKAYEIFKRQVLQGVKSGVDVIVIETMMDLYEAKVAVL
                     AAKENSDLPIFCTMTFDEGGRSFTGCMPECMVATIEGLGVDAIGVNCSLGPKQLLPIV
                     EKIASRATVPVMVQANAGLPNIVDGEAIYDVDAKEFFEGVKKFVEVGATIIGGCCGTN
                     PSFIKEISENINSVTKGCIEKIDKCVVCSPSKFVEVQSPTVVGERLNPTGRKSLQEAL
                     KNENVDYAINLGLEQVNAGAQILGVNVGLPEIDEKKLMPKLIREIQAVVDTPLQVDSS
                     NVEALEQGLRYYNGRTIVNSVNGKEESLESILPIVKKYGSCVVGLTLDEKGIPKKAEE
                     RFEIAKRIVNRAVSYGIKPKDIFIDCLSLTVSAQQEEAIETIKAITMVKTLGVKTILG
                     VSNISFGLPNRKALNASFLTLALGAGLDLAIINPNEYSMMEAINSFKILNNTDKGCIN
                     YINQYSNINNSKKSDSSTKIDKDLPLDILVERGLKDEAKNVTLNLLKEKDENYILDEI
                     LIPALDKVGKRYDSGDIFLPQLIQSAETVKVSLNTIKETLLSKSSNNVSKGKIIVATV
                     KGDIHDIGKNIVKIMLENYGYEVIDLGKDVPIEEVVEVAKKRNIKLVGLSALMTTTVQ
                     SMKDTIQALRDNEINAKVFVGGAVLTEEYAEEMGADYYSKDAKSAVEIAKLNF"
     gene            complement(7772..9001)
                     /locus_tag="ACOIAF_003312"
     CDS             complement(7772..9001)
                     /locus_tag="ACOIAF_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HGZ2670306.1"
                     /translation="MNLNRNLEKYAELAIKVGVNIQPGQILLIKSPIECADFARNALK
                     EAYKCGAKNVYIEWSDEESTLIKYLYAPDEAFHEFPKWTAEQYVDIAKEGGAFLSVYA
                     QNPDLLKDVDPERVANFQKASGKALKEWRSYTLTDKCKWSIVSVPTKDWAKKVFPNLS
                     EDKAIEKLWDAIFKCSRVDGQDPIKAWEEHNENLKSKMDFLNKNNFKTLKYKSSKTDL
                     TLDLPKGHVWLSGASKDPNGISFNPNIPTEEIFGMPHKFKVNGTVYSTKPLVYGGNII
                     DNFFLTFKDGKIIDFSAEKGLDTLEKLIETDEGSHYLGEVALVPYNSPISNTDIIFYN
                     TLYDENASCHFAIGSAYKTCLEGGNNLKDEELDEHGVNDSLTHVDFMIGSADMDIIGE
                     TYDGKQIQIFKNGNWAF"
BASE COUNT         3578 a         1047 c         1540 g         2940 t
ORIGIN      
        1 gtagattgaa tgaattaata gattaaatta atatataaaa tatttaagtt ataattaatg
       61 tattagacaa aataaatact attaagaact attaaaatat agattaaaga taataacata
      121 taataaataa aaaagtaaga ttgtgtagat tatataaaga ttatttacaa ttaatacaat
      181 cttgcttttt tatttaaaaa tttttatctt ctctttatcc catataaaca atttctcaga
      241 attatttaaa gcaccaaata catttctttt aaaatcaggc atctctttat ttaaattagc
      301 aataagattt tttatgtact ctctcctagt atgaccattt gcagggcagg gatttgtgat
      361 aataggatag ttagaatctt ttgaaatttt cttagtcata tactcttcta tgtaaaccat
      421 aggtcttata actgttaaat cctgtctgtc taggtatgtc tttggggaaa aacaatttac
      481 tctaccctca taaaacatag acattaaaaa tgtctcaatt gcatcatctt tatgatgacc
      541 aagagctact ttattacatc caagtttttt agcattatcg ttcaaagcac ctcttcttaa
      601 attagcacat aatgcacaag gattcttttc ttgtcttata tcaaatacaa tttcttttat
      661 gtcagtttga aattcataaa aaggaacatt tatttcttta cacaatttat ctagtggaga
      721 attatcaaca cctccagtat ttaatgttat ggctatcaat tcaaagtttt gtggagaaaa
      781 tctttgataa cttttaagta tatgaagtaa agttaaacta tcttttccac cagaaagtcc
      841 aaccgcaatt ttatcatttt cttgtatcat atcaaaatct tgtatagcct tacgagcttt
      901 acttagaagt ttttgtatag acattttatc accatccttt aaaaatatta aatttaagat
      961 ttatttaaga tttacttata aaatacttaa gtcttatact ctaaaattaa cattatagca
     1021 aacataacaa aaaggagaag atttaattat gaaaaaattt aaattaatag gagccattat
     1081 tttagtagta tttataagtg tatttatatc taaagataga ttacttaaga aagataacga
     1141 tccaatagta gctgctcaac atgattcaga acaagatgtt gaggcaaatt ctcaaaataa
     1201 aaatcactca aatagtatat caaatatatt attggtaaac aagacaaatg gaattagcaa
     1261 aaactatact cctgaaaata ttactaaggt aaacatacct tttgttgaag aagcaacaga
     1321 ggaagaaaaa caaatggcag gagaacctgc aaaggcagtt gaagatttag ttaagcaagc
     1381 taattcagaa ggaatacagt ttttagggtc ttcagcatac agatcttatg atactcaact
     1441 ggatacttat acaagaaggg taaaatctca gggacgagaa aaggcagatg tatatgtagc
     1501 aaagcctggt tacagtgagc accaaactgg actttgtatt gatttaacaa atccagaaag
     1561 atggtttgtt ggaagtacaa aagaagctaa atggttggca gaaaatgctc acaagtttgg
     1621 atttataata agatatcctg aaggtaaaga agatataaca ggtacagcat atgaaccatg
     1681 gcatattaga tatgttggta aggatgctgc cgaggaaatt tattcaaaag gattaacttt
     1741 agaagaatat ttacaaaata aataatataa atgtgtaaat aatttgtatt atattggaaa
     1801 tttagaaatt aataaattta tagattacac taactaaaat atagtataat tttatatgaa
     1861 taaagctata tacaaagatt aagttaggat gtgtaaaaat gaataataca gttcttgttg
     1921 tggatgatga aaaggaaata agagacctta tagagatata tcttttaaat agtggatata
     1981 atgtaattaa agctgaagat ggaaaagagg ctctagatat tttaaaaaag gaaaatattc
     2041 atttaatgat attggatata atgatgccta aaatggatgg gatagaagtt tgtagaaaag
     2101 ttagagaaag tttgaatata ccaatactta tgttaagtgc aaaatcagaa gacatggata
     2161 aaatacaagg gataatgact ggtgcagatg attatttgac aaaaccattt aacccacttg
     2221 aacttactgt gagagtgaag tcgctgatta gaagagcata ttattttagt ggtgctaata
     2281 atgaagataa agatgtcata aaggttggag tagtaattat agataaggaa aaacatagtg
     2341 taacagtggc tggaaatgaa ataattttga catcaagaga atttgatata ttatatttac
     2401 tagcaaataa taaaggcaga gtatatagta cagaagaaat atttgaaaag gtatggaaag
     2461 agaaatatta ccaatctaat aatactgtaa tggttcatat gagcagaata agggataaaa
     2521 ttgaaaaata tacagatgga gaaaaaataa ttcatactgt atggggagta ggatataaaa
     2581 ttgaaaaata aaagtttatt aaatcatatg tttcgaccca tattagatct tataataagc
     2641 tgtgctttaa cttcaatgct tattttaaca agtatgtata taataaaatc attatatgtc
     2701 aatagtgata tgtctgtagt taatcaactt gtatatcaag caagagattt cagaagtctt
     2761 atagggtatg atttatttta tccattttta tttacaatca catcattagc aatttattcc
     2821 attatatcgt acaagagaaa taagcaaatt gtagatttta taagaaagac taatagaaaa
     2881 aatcaaaaat tagaaatcat taaaagtgct gtaaatttaa tggatgaagg taacttagaa
     2941 aaaagtatag atattgagta tgatttagat atattaaagg ataaagaaaa ggatgatatt
     3001 gatgaactag cacataaaat caataatata gtccatcaac ttagaaatat aactattgaa
     3061 gagagacaag ctcagcagac aaaaacagat ttaataacaa atgtgtcaca tgacttaaga
     3121 acacctttga catccataat gggttattta ggagtgatag aagaaggtaa gtataaagat
     3181 gaagttcaga tgatgtatta tgttgatatt gcatatgaaa aatcgaaaaa tttaaatgtt
     3241 cttataaatg atttgtttga attgactaaa atgcaaaata atacaataaa attaaacaaa
     3301 atagaaataa atttagtaga gttattaagt caagtagtat ctcaatttga aatttatttt
     3361 aaacaagaat ttatggttag tagaattaat tttaatgaag aaaaattaat tgtaaaagct
     3421 gatccaaata aattggtaag agcatttgaa aatcttataa ctaatgctgt gaaatatgga
     3481 aaagatgggc attatgtaga tatagtcaca gagaaaaagg aagatatggc ggttgtaaaa
     3541 gtgataaatt atggggaacc aattccagtg ttagatttac caaatatatt tgatagattt
     3601 tatagagttg aaaaatcgag aaatagaaat gttggaggtt ctggattggg tcttgcgatt
     3661 acaaaaaata ttgtaaattt acatgatgga gaaattactg tttcaagcga taaatatcaa
     3721 actgtgtttg aagtacaact gccaataata caatcagctt agttataaaa taaaagtacg
     3781 attatgtgat ataaatatta taacaataaa gtgtttacaa aaataaaaaa gtaatatata
     3841 atttaagtca gtttaataag atacttatca agagttaggg gagagagtta gctcagtgat
     3901 cctataccaa ccttttatca ggtggtcctg ctaacaacga tgagatgact tgaaataata
     3961 tactcttctt atcaataata gataggaaga gtttttttta gaattaatgt aacaaatttt
     4021 aaagtataat ggaggattaa ataatggaaa aagtagaaag ttttaagcta gaccatacaa
     4081 aagtaaaagc tccttttgta agaaagtgta gtgtacttga tggagtaaag ggagataaag
     4141 taactaaatt tgacttgaga tttttacagc caaatgtaga aagttttgga actgctgcta
     4201 tgcacggttt agagcatctt ttagctactt atttaagaga tacattagat ggtgtaatag
     4261 acttatctcc aatgggatgt agaacaggat tttatttaat actatgggga gatgtagatg
     4321 ccaaaacagt taagattggt ttagaagaag cattaaaaaa agttcttgaa tcagataaaa
     4381 tgccagcagc tacagccata gaatgtggaa actacagaga cttatcttta tttggggcaa
     4441 aggaatatgc aaaagatgtt ttagataaag gattttcttt aaatatatat ggagaataat
     4501 ttaattggaa gagaagatat aagcataaat caaagtgaag ttttaagata tttacaatac
     4561 agaggtcaag agatagataa agaccttaaa tatactattg atgaatgtgt agctataact
     4621 aaaaataaaa taaatcctag atatttatct aggatttatc ctattaaaat aaataaagat
     4681 aatgttgtag aattagaggg aacaaattta acattagaga gtaaagatgt atatgaactt
     4741 ttaaaagaat gtaatgagtg tgtaattatg gcagctacta tagggataga tatagaaaaa
     4801 gaaataagac gacactctta ttcaaattta acaaaaggac taataattga ttcatgtgga
     4861 acaacagcaa tagaagaagt gtgtgacatt gttcaaaata atttagaaaa ggttttttta
     4921 aagcaagata aatttataac tatgcgatat agcccaggat atggagattt accaatagaa
     4981 aaaaatatag atatattaaa cgttttgaat gctcaaaaac aaataggtct aacaattact
     5041 tctaatggaa ttatgatacc gagaaagtca gtagtggcta ttataggtat ttctaataca
     5101 agaataaata aatctaagaa agaaaagtcg tgtgaaaatt gtaaaaacta tagaaactgt
     5161 acatatagaa aaggagcgaa ctcttgtgga aataagacaa tatctgaaaa ataatatatt
     5221 aatatttgat ggtgcaatgg gaacaatgct tcaacaaaaa gggttaaagt taggtgaaaa
     5281 tccagaagta tttggtcttc aaaatccaga taaacttata gaaatacata cagcatactt
     5341 agaggcaggt tcgaatgtaa ttcttacaaa tacttttgga tgtaatgaac taaaattaga
     5401 tagtaagtat actgtagaag aagtgattga caatgctgtt ttggtggcta gaaaagctat
     5461 agaaaatgta gataatacta aaccaagata tgtagcttta gatattggtc ctataggaga
     5521 aatgctagag cctatgggta ctttaagttt tgataaagca tatgaaatat ttaaaagaca
     5581 agtactacaa ggcgttaaat ctggagttga tgtaatagta atagagacta tgatggattt
     5641 atatgaagct aaagtagctg tattagcagc aaaagaaaac tctgatttac ctattttctg
     5701 tactatgaca tttgatgaag gtggaagaag ttttactgga tgtatgccag aatgtatggt
     5761 ggctactata gaaggtctag gtgtagatgc aataggagtt aattgttcat taggacctaa
     5821 acaactttta ccaatagttg aaaaaatagc atcaagagct acagtccctg ttatggttca
     5881 agcaaatgca ggtcttccaa atatagttga tggagaggct atatatgatg ttgatgcaaa
     5941 agaatttttt gaaggggtta aaaagttcgt tgaagttggt gctactataa taggtggatg
     6001 ttgtggtacg aatccaagtt ttataaaaga aatatctgag aatataaatt ctgttacaaa
     6061 gggatgtatc gagaaaattg ataaatgtgt agtctgttca ccatcaaaat ttgtggaagt
     6121 acaatcgcct acagttgttg gagaaagatt gaatcccaca ggtagaaaat ctctacaaga
     6181 agcattaaaa aatgaaaatg ttgattatgc tataaattta ggtttagaac aagtaaatgc
     6241 tggagcacaa atacttggag taaatgttgg tcttccagag attgatgaaa aaaaactaat
     6301 gccaaaatta ataagagaga ttcaagcagt agtagataca cctttacaag ttgattcttc
     6361 caatgtagag gcattagagc aaggacttag atattataat ggtagaacaa tagtaaactc
     6421 tgtaaatggc aaagaggagt ctttagaaag tatattacca atagttaaaa agtatggttc
     6481 atgtgtggtt ggacttacac ttgatgaaaa ggggatacct aaaaaggctg aagaaagatt
     6541 tgaaatagca aaaaggatag ttaatagagc agtttcttat gggataaaac caaaagatat
     6601 atttatagac tgtttatcat taactgtttc agcacaacaa gaagaagcaa tagaaactat
     6661 aaaagctata acaatggtaa agacactagg agtaaaaact atattaggtg tttcaaatat
     6721 ttcatttggt cttccaaata ggaaagcgct taatgcttct tttctaacat tagctttagg
     6781 ggcagggctt gatttagcaa ttataaatcc aaatgaatat agtatgatgg aagctatcaa
     6841 ttcttttaaa atattaaata acacagataa aggatgtata aactatataa atcaatatag
     6901 taatataaat aattcaaaaa aatcggattc atctacaaag attgacaaag atttgccatt
     6961 ggatatttta gttgaaagag gattaaaaga tgaagcaaaa aatgtaacac tgaatctttt
     7021 aaaagagaag gatgaaaatt atatattaga tgagatttta attcctgctc ttgataaagt
     7081 aggaaaaaga tatgatagtg gagatatatt tttaccacaa ttgattcaat cagcagaaac
     7141 ggtaaaagtt tcattaaata caataaagga aactttatta agtaaaagta gcaataatgt
     7201 atctaaagga aaaataatag ttgctacagt aaaaggtgat atccatgata ttgggaaaaa
     7261 tatagtaaaa ataatgctag aaaattatgg atatgaggtt atagatttag gtaaagatgt
     7321 ccctattgaa gaagtagtgg aagttgctaa aaagagaaat ataaaattag taggattaag
     7381 tgcacttatg actacgactg ttcaaagtat gaaagataca attcaggcac tgagagataa
     7441 tgagataaat gcaaaggtat ttgtaggagg tgcagttttg acagaagaat acgctgaaga
     7501 aatgggtgct gattattatt caaaggatgc aaagtctgct gttgaaatag caaagttaaa
     7561 tttttagcat ataaattaaa ataaaggttt tactaaatag gatattttat atataatata
     7621 aatataagtt ttagatactt atgtttaaaa caataggaat taatctataa taatgagatt
     7681 aattaatata aaaaatattt atcatcaaca ttaaaaaggg ctgggaagtc tggtataact
     7741 tccatcagcc ctttttagtt aagatcatat gttaaaaagc ccaattacca tttttaaaaa
     7801 tctgtatttg ctttccatca taagtctcac caataatatc catatcagca gaaccaatca
     7861 taaaatctac atgtgttaaa ctgtcattta caccatgttc atctaattct tcatctttta
     7921 aattgtttcc accttccaga caagtcttat aagcagaacc aattgcaaag tggcaagaag
     7981 cattttcatc atatagagta ttataaaaaa ttatatccgt attggatatt ggtgagttgt
     8041 atggaacaag agcgacttct cctaaataat gagagccttc atccgtttca ataagttttt
     8101 ctagtgtatc tagtcccttt tcagcagaga aatctattat tttaccatct ttaaatgtta
     8161 agaagaaatt gtcaattatg tttccaccat aaacaagagg ttttgtacta taaacagttc
     8221 catttacttt aaacttatgt ggcattccaa aaatctcttc agttggaata ttcggattaa
     8281 aacttattcc attagggtct ttggatgctc cacttagcca aacatgaccc tttggcaagt
     8341 ctagagtcaa atcagttttt gaggatttat attttaaagt tttaaagtta tttttattta
     8401 gaaagtccat tttagattta agattttcat tatgttcttc ccatgctttt atagggtcct
     8461 gaccatctac tcttgaacat ttaaatatag catcccacag tttttctatt gctttatctt
     8521 ctgataaatt tggaaacact ttttttgccc aatcttttgt tggcacagag actatactcc
     8581 atttacattt atcagttaga gtgtaagaac gccattcttt taaagctttt ccagatgcct
     8641 tctgaaaatt tgcaactctt tcagggtcaa catcttttaa taaatctgga ttttgggcat
     8701 agactgataa aaatgcccca ccttcttttg ctatatctac atattgttct gctgtccatt
     8761 tggggaactc atgaaatgct tcatctggag catataagta tttaataaga gtactttctt
     8821 catcagacca ctctatataa acatttttag caccacattt atatgcttct ttaagtgcat
     8881 ttcttgcaaa atcagcacat tctattggtg actttataag tagaatttga cctggctgta
     8941 tgttaactcc aacttttatt gctaattcag cgtatttttc taaatttcta tttaaattca
     9001 tatttatcct cctatatttt taatattgaa taacttataa agtattataa cattacttat
     9061 gtttttaata ttgaacaact tataaaatat tataacttta cttag
//
LOCUS       DBGJFF010000146         8775 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.149, whole genome shotgun sequence.
ACCESSION   DBGJFF010000146 DBGJFF010000000
VERSION     DBGJFF010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8775
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.149"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            371..496
                     /locus_tag="ACOIAF_003313"
                     /pseudo
     CDS             371..496
                     /locus_tag="ACOIAF_003313"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
     gene            551..817
                     /locus_tag="ACOIAF_003314"
     CDS             551..817
                     /locus_tag="ACOIAF_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HGZ2670307.1"
                     /translation="MAFIFINTDLSKSMKEFKKTILEYDTVLECHHISGEYDYLIKVL
                     AEDTSNLEEFISVTLKEIGGIQKANTTIVLSTIKEEFNTIKDIF"
     gene            866..1450
                     /locus_tag="ACOIAF_003315"
     CDS             866..1450
                     /locus_tag="ACOIAF_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HGZ2670308.1"
                     /translation="MKVLVFLAKGFETMEFSVFVDVMGWARNDYGHDIDVVTCGFKKQ
                     VMSTFNIQVLVDKTIEEVCVDDYDALAIPGGFEEFGFYDEAYDSSFLNLIREFNSKEK
                     IIASICVAALPVGKSGVLKNRKATTYHLKNGKRQRQLSEFDVNVVNEPIVVDKNIITS
                     YCPETAPHVAFKLLEMLTSKEQMDEVKLAMGFKL"
     gene            complement(1455..1778)
                     /locus_tag="ACOIAF_003316"
     CDS             complement(1455..1778)
                     /locus_tag="ACOIAF_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HGZ2670309.1"
                     /translation="MKKCLDNYSCPIEATLALIGGKYKTLILWHLKDTILRFNELKKL
                     IPKATPKMLTQQLRELESDGLIIRVVYPVVPPKVEYSLSDFGKSIIPILDSMCDWGSD
                     YLENL"
     gene            1930..2448
                     /locus_tag="ACOIAF_003317"
     CDS             1930..2448
                     /locus_tag="ACOIAF_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HGZ2670310.1"
                     /translation="MNFVELAKKRYSCRNYQDRKVEKEKLEKVLDVARIAPTGGNRQP
                     QRLIVIQEKEGINKLSKAANIYDAPLAILVCGDKDKVWTRPFDGKQLTDIDTSIVTDH
                     MMLQATELGLASVWVCYFNPDIIREEFSLPDNLEPINILLMGYESKIPESPERHEKTR
                     VPLSEIVSYETL"
     gene            complement(2624..3292)
                     /locus_tag="ACOIAF_003318"
     CDS             complement(2624..3292)
                     /locus_tag="ACOIAF_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HGZ2670311.1"
                     /translation="MLEVSHKVKEIYPNIKFGVMVINITHSKPNKENFLVLKNSTIKN
                     IIEQHPEYNRKEKIKTEPVSSYIKYYKKFKKTYPVLLQLESILLKSKGIPDVGVTIES
                     MFLAELKNLLLTAGHDLDKMELPLKIELANGSEHFYGIGGKEHILTKDDLFLSDNIGI
                     LSSILNGPDNRTAITKDTKNIMYFVYGPDKISEKQIQDHLNDIKHYISSAFPDLKVNS
                     IDIF"
     gene            3407..4822
                     /locus_tag="ACOIAF_003319"
     CDS             3407..4822
                     /locus_tag="ACOIAF_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HGZ2670312.1"
                     /translation="MWITINRNSNISLSRQIYCEIKDMILDGSLKFEQKLPSSRTLSK
                     ELSVSRNTVLDAYNQLIAEGYLETRNGYGTIVAKGISGHKVDFKLNDTFRDEKDLHTN
                     KQKNYIDFRSGVPELKLFPRKELSKLYKQILYDLSDSDFRYGSTAGVWVLRVEISKYL
                     FRTRGIRCNPKNIMIVSGSTQGLSLISQLLYKSNKKVAVEDPIHKGLLKVISSIGYSV
                     VGIKVDDKGMNTNLIKSIKGISFVYTTPSHQYPLGGILPIQRRLELIKCATENDYYII
                     EDDYDSEFRYEGQPISSLYELSPNKVIYIGSFSKILSPAIRVGFMLLPDNILESYKTL
                     KMYSDVHTETILQHVLAEFIGNGGLEKYIFRMKKLYNRKRKYLIKVLNTEFKNEFKIK
                     GEAAGLHIVAHFYNVKFTESLIHKIFEAGIKVYPIQQYAIENILEYDYEIILGYAHLS
                     FDEIEKGVKILSKIIHMNQGT"
     gene            5021..5503
                     /locus_tag="ACOIAF_003320"
     CDS             5021..5503
                     /locus_tag="ACOIAF_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGZ2670313.1"
                     /translation="MKTNTDVIMEIIDYIEENLTENLNLDKIAYIANYSKYHLHRMFT
                     NVVGCTIHQYIQRRRLTEAARQLIFTDKYIIDIAITVGYSTHQSFTLAFRNLYKESPQ
                     VYRNKDEFYPIQQRFVLNLNHQIQSEKCISYANMNYKQRRDEINSYLNFNINCEVKVA
                     "
     gene            5500..6084
                     /gene="clpP"
                     /locus_tag="ACOIAF_003321"
     CDS             5500..6084
                     /gene="clpP"
                     /locus_tag="ACOIAF_003321"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HGZ2670314.1"
                     /translation="MSLVPHVIEQTGQGERSYDIYSKLLKDRIIFIGEEINDAIASLV
                     VAQLLFLESEDPDSDIIIYINSPGGSATSGFAIYDTMNYVRCDVSTICIGMAASMSAF
                     LLAGGTHGKRCALPNSEIMIHQPMGGAKGQATDVKIAVDNILKIKERLDKILSENTGK
                     SIEDIRRDTDRDNFMTALEAKEYGLIDYIMNRNE"
     gene            6396..7472
                     /locus_tag="ACOIAF_003322"
     CDS             6396..7472
                     /locus_tag="ACOIAF_003322"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HGZ2670315.1"
                     /translation="MITISLCMIVKNEEEVIGRCLECVKDIVDEIIIVDTGSTDSTKE
                     IVSKYTDMVYDFEWIDDFSAARNFSFSKASKDYTMWLDADDIILEADREKLLKIKESL
                     DTSIDIVMAKYNVSFDENGNPTLSYYRERLFKRSSNHKWVDPIHEVVPLFGEVFYSDI
                     AISHKKLHRQDPLRNLRIFEKMISEGKTLEPRHQFYYSRELYYNARYKEAIEGFTKFL
                     DSSRGWIEDCISACRDLATCYYLINDEKSALYSLFRSFEFDEPRAEICCDIGKHMFDR
                     QKYKEAIFWYKVALTRDKNDTNGGFKSNDCYGYIPSIQLSVCYDRLGESDKAIYYHEK
                     TKEIKPNDSAVLHNENYFSKFKNS"
     gene            7539..>8775
                     /gene="bclA3"
                     /locus_tag="ACOIAF_003323"
     CDS             7539..>8775
                     /gene="bclA3"
                     /locus_tag="ACOIAF_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA3"
                     /protein_id="HGZ2670316.1"
                     /translation="MLLIMSRNKYFGPFDDNDYNNGYDKYDDCNNGRDDYNSCDCHHC
                     CPPSCVGPTGPMGPRGRTGPTGPTGPTGPGVGATGPTGPTGPTGPTGNTGNTGATGLR
                     GPTGATGATGPTGATGAIGFGVTGPTGPTGATGATGADGVTGPTGPTGATGADGITGP
                     TGATGATGFGVTGPTGPTGATGVGVTGATGLIGPTGATGTPGATGPTGAIGATGIGIT
                     GPTGATGATGADGATGVTGPTGPTGATGADGVTGPTGATGIGITGPTGPTGATGIGIT
                     GATGLIGPTGATGTPGATGPTGATGPTGVGVTGATGATGATGADGATGVTGPTGATGA
                     TGANGLVGPTGATGAAGTPGATGPTGATGPTGVGITGATGATGATGPTGADGATGPTG
                     ATGNTGADGVAGPTGATGAT"
BASE COUNT         3354 a         1107 c         1593 g         2721 t
ORIGIN      
        1 acaaagttat ttgaagactc gctatcaata aaattttata gtctataaga aaattaagat
       61 tatgagtttt ataattttga tatagggata ctattttcat aaatattaca acatgtagaa
      121 gtatatgtct agtattgttg aattttcaat gatatattaa ggttctaata tattatacaa
      181 aataaaaaca ttaattcaac ttaaataatt ttaagtatta tatgtgtttt gattaaagaa
      241 aactaaaatt ttaaggaaaa ataacatatt tgtattaaat tttattgaaa tgattaaatt
      301 tatttactta atgtaaatta ttagctaaaa ttgtattaaa taattaagga ggtgagtagc
      361 tctggatgaa atagatttaa aaataattaa ttctctaaaa aagaatagta gggcttcaac
      421 ctctgaaata agcagacaaa ttaatttatc agtaccagca gtctcagagc gtataaggaa
      481 aattgagcag tcaaatttaa ttcaaaagta cactattcaa attaatcggg aaaaaacaaa
      541 atataagctt ttggctttta tatttatcaa tactgattta tctaaaagta tgaaagaatt
      601 taagaaaact atattggaat atgatactgt attagaatgt catcatattt caggagagta
      661 tgattactta ataaaagtat tagcagaaga tacaagtaat cttgaagagt ttatatcagt
      721 gactttgaag gaaattgggg ggattcaaaa agctaataca accattgttt tatctacaat
      781 taaagaagaa tttaatacaa taaaagatat tttttaaata atttactagt aaagtgttaa
      841 ttatatttta ggaggaaaat agaatatgaa agttttagtt tttttagcaa aaggttttga
      901 aactatggaa tttagtgtat ttgtagatgt tatgggatgg gctcgtaacg attatgggca
      961 tgatattgat gtagtgactt gtggatttaa aaaacaagtt atgagcacat ttaatataca
     1021 agtattagtt gataagacga tagaagaagt atgtgttgat gattatgatg ctctagctat
     1081 tcctggagga tttgaagaat ttggatttta tgatgaagca tatgattcat cttttttaaa
     1141 tctaataagg gagtttaatt caaaagaaaa aataatcgct tctatttgtg tggcagcatt
     1201 gccagttgga aaaagtggtg ttttaaaaaa tcgtaaagct actacatatc atttgaaaaa
     1261 tggtaaaaga caaagacagt taagtgagtt tgatgtaaat gtagttaatg aaccaattgt
     1321 tgtagataaa aatattataa cctcatattg tccagaaaca gctccacatg tagcttttaa
     1381 gcttttagaa atgttgacat ctaaagaaca aatggatgaa gttaaattag caatgggatt
     1441 taaactgtaa tggtttatag attttctaag taatcagaac cccagtcaca catggaatct
     1501 aaaattggaa taatactctt tccaaaatca gatagcgagt attctacttt tggaggaaca
     1561 acaggataaa ccactcttat aattaaacca tctgattcta attcacgtaa ttgttgagtt
     1621 aacatcttag gcgttgcttt aggaattaat ttttttaatt cattgaacct caatatagtg
     1681 tctttaagat gccaaagtat aagtgtctta tatttaccac caatcaatgc aagtgttgct
     1741 tctattgggc aactgtaatt atctaaacat tttttcattt ttgggctcct ttatttattt
     1801 taaagtatct ttttaggtac tatattacaa aaaagtgcat tattgtaata tagtaccata
     1861 gtgataaaat tatagcatag gttaaataaa tcatcaactt agcctaataa aacaataggg
     1921 ggtaaataaa tgaatttcgt tgaacttgca aaaaaaagat attcttgccg taattatcaa
     1981 gatagaaagg ttgaaaaaga aaaacttgag aaggttttag atgttgcaag aatagctcca
     2041 actggtggta atcgtcaacc tcaaagattg attgttattc aggaaaaaga aggtataaat
     2101 aaactttcta aggctgccaa tatatatgat gcgccattag caattcttgt atgtggagat
     2161 aaagacaaag tttggacaag accttttgat gggaaacaac ttacagatat tgacacaagt
     2221 atagttacag accatatgat gttacaggca actgaacttg gattggcaag tgtttgggtg
     2281 tgctatttta atccagatat aattagagaa gagtttagtt tgccagataa tctagaacca
     2341 ataaatatat tattgatggg atatgaatct aagataccag agagcccaga gagacatgaa
     2401 aaaactcgtg ttcctttaag tgaaattgtt tcttatgaaa ctctttaatt ataaaattaa
     2461 gttattcata ggataaatgt aataaagtgt ttgttataaa caaaattcaa aataaataat
     2521 atctacatat aattaatttt agtgaagtat atctagttta actagaatgt atacttcact
     2581 aatttattta ttcaaaatat ttatgaggat tttaatatat taactaaaat atatcaattg
     2641 aattaacttt taaatctgga aaagcacttg aaatatagtg ttttatatca tttaaatggt
     2701 cttgaatttg cttttctgat attttatcag gtccatatac aaaatacata atatttttag
     2761 tatcttttgt tatagctgtt cgattatcag ggccattaag aatactagat aatataccaa
     2821 tattatctga taaaaataaa tcatcttttg ttaaaatatg ttctttacca cctataccat
     2881 aaaaatgttc acttccattt gctaattcta ttttcaaagg taattccatt ttatcaaggt
     2941 catgacctgc tgttagaaga agatttttca attctgctaa aaacattgac tcaattgtta
     3001 cacctacatc tgggatacct tttgatttta atagaataga ttctaattga agcaatacag
     3061 ggtaagtttt cttgaatttt ttataatact ttatataact tgaaacaggc tctgttttaa
     3121 ttttttcttt gcgattgtat tctgggtgtt gttctataat atttttaatt gtagagtttt
     3181 taagaactaa aaaattttct ttattaggtt ttgaatgtgt aatatttata accataactc
     3241 caaatttaat attgggataa atttctttaa ctttatgtga tacctctaac ataatataga
     3301 ctccttttta ttttatattt tatgatataa tcttagcaaa ttaaaagtgg attttatagt
     3361 tccattttta tagtaatttt atagaaccac ttttggaggt attagaatgt ggattacgat
     3421 taatagaaat agtaatatat cattatcaag acaaatttat tgtgagataa aagatatgat
     3481 tctggatgga agcttaaagt ttgagcaaaa actgccatca tctaggactt tatcaaaaga
     3541 actatctgta tctcgtaata cagttttaga tgcatataat caattaattg cagaaggata
     3601 tttggaaaca aggaatggat atggaacaat tgtagctaaa ggaatatcag ggcataaagt
     3661 tgattttaag ttaaatgata cttttagaga tgaaaaagat ttacatacta ataaacaaaa
     3721 aaattatata gattttcgtt caggagttcc agaattaaaa ctatttccac gcaaagaact
     3781 ttcaaagcta tataaacaaa tactgtatga tttatcagat tcggatttca gatatggaag
     3841 tacagctggt gtatgggttt taagagtgga aatatctaag tatttatttc gtactagagg
     3901 aattagatgt aatcccaaaa acattatgat tgtttctggt tcaacacaag gtttatcttt
     3961 aatttcacag cttttataca aaagtaataa aaaggttgct gtagaagacc ctattcataa
     4021 aggcttatta aaggttattt catctattgg atattcagtt gttggaatta aagtagatga
     4081 taagggaatg aataccaact taataaaatc aataaagggg atttcttttg tttatactac
     4141 accttctcat caatacccat taggagggat tttacctatt caaagaagac tagagcttat
     4201 taaatgtgca acagagaatg attactatat aattgaagat gattatgata gtgaatttag
     4261 atatgagggt caaccaatta gttctcttta tgaacttagc ccaaataaag taatttacat
     4321 aggttctttt agtaaaatat tatctccagc tattcgtgtt ggatttatgt tattaccaga
     4381 caatatttta gaaagctata aaaccttaaa aatgtactct gatgttcata cagaaacaat
     4441 attgcaacat gtattagctg aatttattgg aaatggaggt ttagagaagt acatatttag
     4501 aatgaagaag ttgtacaata gaaaacgtaa gtacctcata aaagttttaa acacggaatt
     4561 taaaaatgaa tttaaaataa aaggtgaagc agcagggctt catatagttg ctcatttcta
     4621 taatgttaaa tttacggaaa gtttaattca taaaattttt gaagcaggta taaaagttta
     4681 tcctatacaa caatatgcaa ttgagaatat attagaatat gactatgaaa ttatactagg
     4741 ttatgctcat cttagttttg atgaaattga aaaaggtgta aaaatactca gcaaaataat
     4801 acatatgaat caaggtacat agataaaaca tgtagcaatc tacatataat agatgtaatt
     4861 aagtttaaac tttaaaagaa atatatatta atatttttgt ataaaaatac ctgatatttt
     4921 tttatcgata ctttattgct atacactaaa ttaagatagc aatttatatt aagaaaaaac
     4981 tttttgcata ggttataata cttaaaagga gaggatagag ttgaaaacaa atacagatgt
     5041 tattatggag ataattgatt atatagaaga aaatcttaca gaaaatctaa atttagataa
     5101 aatagcttat attgctaatt attcaaaata ccatttacac agaatgttta caaatgttgt
     5161 aggatgtact atccatcaat acatacagag gaggagactt actgaagcag ctagacaact
     5221 tatatttaca gacaaatata ttatagacat agctattaca gtaggatatt ctacacatca
     5281 atcttttaca ctagcattta gaaatttata taaagaatct ccacaagtat atagaaataa
     5341 agatgaattt tatcctattc aacagagatt tgttttaaat ctaaatcatc aaattcaaag
     5401 tgaaaaatgt atttcttatg caaatatgaa ttataaacaa agaagagatg aaatcaattc
     5461 ttatcttaac tttaatatta attgtgaggt gaaagtagca tgagtttagt gccacatgta
     5521 attgaacaaa caggtcaagg ggaaaggtct tatgatatat attcaaaatt attaaaagat
     5581 agaattatat ttataggaga agaaattaat gatgctattg caagtttagt agtagctcaa
     5641 ttactgtttt tagaatcaga agacccagat tcagatataa ttatatatat aaatagccct
     5701 ggtggctctg ctacatcagg atttgctata tatgatacta tgaattatgt cagatgtgat
     5761 gtatcgacta tttgtattgg tatggctgca agcatgagtg cttttttact tgcaggagga
     5821 acacatggaa aaagatgtgc tttacctaat tcagagatta tgattcatca gccaatggga
     5881 ggagctaaag gtcaagcaac agatgtaaaa atagctgtag ataatatatt gaaaataaaa
     5941 gaacgattag ataaaattct ttctgaaaat acaggtaaat caatagaaga tattaggaga
     6001 gatacagata gagataattt tatgactgca ctagaggcta aagagtatgg gctgattgat
     6061 tatataatga ataggaatga ataaagtatc aaagtatcat attaaaatgt ctgttttaca
     6121 aatatatata taatattaaa taagagtgtc tcaaaatgaa ctttttagtt tatgggacac
     6181 tcttttttgt atgaaattaa atatggtttt atcatttcaa caattattac caaatttcaa
     6241 gtttaataaa ttctactata aaaaatacat aaaagataag gttctttagt aactttctgt
     6301 cgtcaaaagt ttttataaat ttaataacaa aacaagagaa aataccatat attacttatg
     6361 tctgcaatat atagaagaca aaggagttgt acattatgat tacaataagt ttgtgcatga
     6421 ttgttaagaa tgaggaagaa gttataggca gatgcttaga atgtgtaaag gatatagtag
     6481 acgaaattat aattgtagat acaggttcta cagattccac aaaagaaata gtctcaaaat
     6541 atacagacat ggtgtacgat ttcgaatgga tagatgattt ctctgctgca agaaattttt
     6601 cattttctaa agcttctaaa gactatacta tgtggcttga tgcagatgac ataattttag
     6661 aagctgatag agaaaaatta ttaaagatta aagaaagctt ggatacttcc attgatattg
     6721 ttatggcaaa atacaatgta tcctttgatg aaaatggtaa cccaactctc tcgtattata
     6781 gagaacgatt gtttaaaaga agttctaatc ataaatgggt tgaccctatt catgaagtag
     6841 tacctctttt tggagaggta ttttattctg acattgcaat atctcataaa aagcttcata
     6901 gacaggaccc tctaagaaat cttagaattt ttgaaaaaat gatttcagaa ggaaaaacat
     6961 tggagccaag acaccaattc tattattcta gagagttgta ctataatgct aggtataaag
     7021 aagcaataga aggttttact aagtttttag actcatcaag aggatggatt gaggattgta
     7081 tcagtgcttg cagagattta gccacatgtt attacttaat taatgatgaa aaaagtgcct
     7141 tatattcatt gtttagaagt ttcgaatttg atgagccaag agcagaaatt tgttgtgaca
     7201 taggaaagca tatgtttgat agacaaaaat ataaagaagc cattttttgg tataaggtag
     7261 cattaacaag agataagaat gatactaatg gaggttttaa gtcaaatgat tgttatggat
     7321 atataccatc tatacaatta agtgtctgtt atgacaggtt aggagaatca gataaagcta
     7381 tttattatca tgaaaaaaca aaagaaatca aacctaacga tagtgctgtt ttgcacaatg
     7441 aaaattattt tagtaaattt aaaaatagtt gataggtcac aatcatacta aatttgacat
     7501 attatatata gagcttaaaa gctcaaatat atcaggaggt gcttttaata atgagtagaa
     7561 ataaatattt tggaccattt gatgataatg attacaacaa tggctatgat aaatatgatg
     7621 attgtaacaa tggtcgtgat gattataata gctgtgattg ccatcattgc tgtccaccat
     7681 catgtgtagg tccaacaggc ccaatgggtc caagaggtag aaccggccca acaggtccaa
     7741 cgggtccaac aggtccagga gtaggggcaa caggcccaac aggaccaacc ggtccgactg
     7801 gtccaacagg aaatacaggg aatacaggag caacaggatt aagaggtcca acaggagcaa
     7861 caggggcaac aggcccaaca ggagcgacag gagctatagg gtttggagta acaggtccaa
     7921 caggcccaac aggagcgaca ggagcaacag gagcagatgg agtaacaggt ccaacaggtc
     7981 caacgggagc aacaggagca gatggaataa caggtccaac aggagcaaca ggggcaacag
     8041 gatttggagt aacaggtcca acaggcccaa caggagcaac aggagtagga gtaacaggag
     8101 caacaggatt aataggtcca acaggagcga caggaacacc tggagcaaca ggtccaacag
     8161 gggcaatagg agcaacagga ataggaataa caggtccaac aggagcaaca ggagcaacag
     8221 gggcagatgg agcaacagga gtaacaggcc caacaggccc aacaggggca acaggagcag
     8281 atggagtaac aggcccaaca ggagcaacag gaataggaat aacaggccca acaggtccaa
     8341 caggagcaac aggaataggg ataacagggg caacaggatt aataggtcca acaggggcaa
     8401 caggaacacc tggagcaaca ggtccaacag gagcaacagg cccaacagga gtaggagtaa
     8461 caggagcaac aggagcaaca ggggcaacag gagcagacgg agcaacagga gtaacaggtc
     8521 caacaggagc aacaggggca acaggagcaa atggattagt aggcccaaca ggagccacag
     8581 gagcagcagg aacacctgga gcaacaggtc caacaggagc aacaggccca acaggagtag
     8641 gaataacagg agcaacaggg gcaacaggag cgacaggtcc aacaggagca gatggagcaa
     8701 caggtccaac aggagcaaca ggaaatacag gagcagatgg agtagcaggt ccaacaggag
     8761 caacaggagc aacag
//
LOCUS       DBGJFF010000147         8766 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.150, whole genome shotgun sequence.
ACCESSION   DBGJFF010000147 DBGJFF010000000
VERSION     DBGJFF010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8766
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.150"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            190..957
                     /locus_tag="ACOIAF_003324"
     CDS             190..957
                     /locus_tag="ACOIAF_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HGZ2670317.1"
                     /translation="MGEIINALDRALDIILLLYHEKREMGITEISKAMGVYKSTVHRT
                     LVTLENKGFVIQNAENGKYWLGINLYAIGMVVGEKMSLTEIVKPYTKKLNQEFNEVVN
                     VSILEERAQDSPRSIIIHKEYGSNQLLSVNPSVGSSSECYCSAVGKCLMAFNDSIDFE
                     KYRKTPIHKYTEHTIDNWDDMMLFLAKIKEQGYAIDDEELEHGLTCIGAPILDKNNKA
                     IAAISLSGPTIRMREGDFEYKIKRVIETAKSISELFR"
     gene            1240..1437
                     /locus_tag="ACOIAF_003325"
     CDS             1240..1437
                     /locus_tag="ACOIAF_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HGZ2670318.1"
                     /translation="MEKVKLSVERCKGCYLCIEACKKDAISLSDYTNKKGFVTIKVDE
                     EKCIQCGACYTMCPDLVFEIL"
     gene            1466..2542
                     /gene="vorB"
                     /locus_tag="ACOIAF_003326"
     CDS             1466..2542
                     /gene="vorB"
                     /locus_tag="ACOIAF_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HGZ2670319.1"
                     /translation="MQSLLIKGNEALAEGAIRGGCRFYAGYPITPQSEILEMMAWRQK
                     EVGGVFIQGESEIASVNMVMGASALGARAMTSSSGPGFSLKQEGIAYWVSAGLPAVVA
                     CVQRYGIGDGFIGAGQDNYWQAVKGGGNGDYHLIVLAPNSVQENMDMAYESFELAEKY
                     MHPVLILSDATIGQMVEPCIAPPMKEYDMDKAPWAAKGTPVGEKNKVITDITYFEDQD
                     TWQKGYIEKMRKVQEDEQRWEEIEIEDAEVVFVAYGISSRIAEGAVRDARAKGMKLGL
                     IRPKTLWPYPRKAFKKLSDSLKGLVTVEMNMMAQMKEDVIIACDNRFPVYSVATAQYM
                     AEVNQLIDFAQQIIDGKAKQEEVF"
     gene            2545..3264
                     /locus_tag="ACOIAF_003327"
     CDS             2545..3264
                     /locus_tag="ACOIAF_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HGZ2670320.1"
                     /translation="MAKKAPAIMMCENMFCGGCGHSIVDRIIGEVLTEMDICQDTIIC
                     SDIGCNHMFQFSMNVDVVVPPHGKMGAAMAAMKRVRPDKYIFTIAGDGGAYAIGLGET
                     MSAATRNDNVIFLVMNNNMFAMTGGQMAPTTIEGQYTASTPAGRNYADHGSTFDVVKV
                     MGNLDIAYLARGTITNPKEINTTKRYIKKALEKQKEKKGFCLIEVLVPCPTNWGLKPV
                     DAMKRIENELVPKYPLGEFID"
     gene            3277..3816
                     /locus_tag="ACOIAF_003328"
     CDS             3277..3816
                     /locus_tag="ACOIAF_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HGZ2670321.1"
                     /translation="MLNEIICAGFGGQGVLTAGKIIMYVAYKAEKKITWFPSYGNEMR
                     GGSANCNVVVSDEKIANPCVKNPDILLGMNSVSVDKYESLMKKNGYMFVNESLVSKDR
                     KYREDINVIKVPVTEIAQNLGNEKAANICMLGAMVKSLDLFTKEEFESGMCEYFENQG
                     KGKFNSKNVEAFEAGYDYE"
     gene            3973..5031
                     /gene="buk"
                     /locus_tag="ACOIAF_003329"
     CDS             3973..5031
                     /gene="buk"
                     /locus_tag="ACOIAF_003329"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HGZ2670322.1"
                     /translation="MKVLVINPGGTSTKISVFQDENEILKKNITHTREDLKNFSKVFD
                     EYDYRKQLIVDILSSENHSINSFDAVVGRGGLMKAIKGGTYTVSEEMIEDMRNEINGE
                     HASNLGALLAKTIADEIGVQSFVVDPVSVDEFDDVSRITGISDIEKASWLHALNHKAV
                     SRKIAQKLGGKYEDYNFIVAHLGSGISIVAHKQGKMIDGSGGRSDGPFSPERSGGLLA
                     YPLIELCYSGKYTHDEMVAKVSSIGGMYDYLGTKDMIEIENRIKEGDEKAKLIMDAFI
                     YQVAKEISMYGASLSGNVDRVILTGGISHSEMVVKGVAEKVSYLAPIEIVPGEMEMEA
                     LALGALRVLNGEEIAKTY"
     gene            5049..5942
                     /locus_tag="ACOIAF_003330"
     CDS             5049..5942
                     /locus_tag="ACOIAF_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HGZ2670323.1"
                     /translation="MLKDFNNLLEEAKKLEPVIVSVAVAEDKEVLKAVKSATDLNIIK
                     PILVGDKDKIESISREIGLKGHEIIGCQNPDESVKKAVEIVKNKEAKVLMKGLVNTSV
                     YMRAILNKETGLRTGRLLSLLAVYELPQYHKLLYCTDSGINVSPNLNQKKDIMTNALL
                     TMKSIGMENPKTAILTANEMVDPKIQSTVDANLLVEMEKSGEIPKCIVEGPIAFDVAF
                     DAHAAEHKGIKSNIAGDVDLLVFPNIEAGNILGKSWLRFNKAKWAGIVLGATNPIILG
                     SRSDTAEIKINSIALACLASQ"
     gene            5972..7180
                     /locus_tag="ACOIAF_003331"
     CDS             5972..7180
                     /locus_tag="ACOIAF_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HGZ2670324.1"
                     /translation="MKYAKRMDMVKASAIRDSQKKIAQKVASGGTVISFAAGLPDPNL
                     FPIEEISEITQKVLNERGKFALAYGPTKGEDELLDLLVKRMKEEENIDTKAENIIITT
                     GSQQGIALSAMILLEKGDVVVTENPSYLGAINAFRPYECDFLGVDTDEEGMVTDDLDK
                     LLSENSNIKVIYVIPTFQNPTGKTWSLQRRKDFMEVVNKYDVIVIEDNPYGEIRFTPE
                     PLPTLKSLDTKDKVLYLGSFSKVLCPGFRVAWMCGNVDIIDKAELLKQGIDLQSNQFS
                     QVQVIEFLKKYNLNEHIEKIRAEYKKRCLLMLDAMKKYFPEEAKYTEPDGGMFIWVEL
                     PESINVDDLLDKAIDAGVAYVSGESFFANNGPKNTMRLNYTTMSEEQIVKGVEILAEV
                     IKKELNYACE"
     gene            7204..8463
                     /locus_tag="ACOIAF_003332"
     CDS             7204..8463
                     /locus_tag="ACOIAF_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGZ2670325.1"
                     /translation="MGEKENGIKFGKLMFQLVIIAISWELVYIIPFIQYTLYDPILKA
                     LECTNTQLGFLLTIYGLGNIFGAPIGGWLADRFDYRKIILGSVFLNGVVSFLFAFNMN
                     YPFAVATWVGCAITSLVMNYPSMVKILRVIGKDNQGKVYGFNEAMVGVSGVVMGAIFL
                     YIYTCFATPTLGMRWVMISLGILSIAMCPVLWFVIKDVDIKEEREEAGEKMSAGDFIT
                     VLKSPNTWLVGISIFCVYSFTVTMSYFTPYITSVLGGSVALSGALAIIRQHGLKLFGA
                     PFGGYCADKLKSPTKVLLPIYVFGIAVIVLFLVLPASTPMTIFIALTFVVGILGYMGK
                     GIYYAVQDEVKVPVKYSATTIGIAAALGFSPDVFQFALIGHWIDTYGNKGYTYTFIFQ
                     IAILVIGILSCLYILKVKKRRASKLEA"
BASE COUNT         3404 a          969 c         1746 g         2647 t
ORIGIN      
        1 gaataattct attaaaagta agaataataa ggacatagtg agtattttgt ttttatttta
       61 caaattaagt ataatatttt tgttttataa aatataatta agataaatag ataattttgt
      121 aattttaata tataatattt agtagagtgt gggagtagca cttaatattt gcaggaggag
      181 tttttgtata tgggagaaat aataaatgca ttagatagag ccctagacat aattttgctg
      241 ttatatcatg aaaagagaga aatgggaata actgaaataa gcaaggctat gggagtatat
      301 aaaagtactg tacatagaac tttggttact cttgaaaaca aaggttttgt tatacaaaat
      361 gcagagaatg gtaagtattg gctaggaata aatttatatg caataggtat ggttgtggga
      421 gaaaaaatgt cattgactga aatagtaaaa ccatatacga aaaaattaaa tcaagaattt
      481 aacgaagtag ttaatgtatc tatattagaa gaaagagctc aagattctcc aagaagtatc
      541 ataatacata aagagtatgg ttcaaatcaa ttactttcag taaatccatc cgttggttca
      601 agtagtgaat gttattgttc agcagttggt aaatgtctta tggcatttaa tgattctata
      661 gattttgaaa aatatagaaa aacaccaata cacaagtata cagaacatac aattgataat
      721 tgggatgata tgatgctttt tttagcaaaa ataaaagaac aaggatatgc tatagatgat
      781 gaagaactag aacatggact aacttgtatt ggagcaccta tcttagataa aaataataaa
      841 gctattgcag ccataagttt gtcaggtcca actataagaa tgagagaagg ggactttgag
      901 tataagatta aaagagttat agaaactgca aaaagtatat ctgaactttt tagatgattt
      961 ttaatctcga taataaaaat aggctatatt taaattgtta tttgtaaata tcaatttaaa
     1021 tgtagcctat ttttctttaa tctaaaatta aatattctta aaatttaaaa tgttattgaa
     1081 atatataagc gaatgtgtta tcttttaatt aagcaataaa aatagaacaa ggttctatac
     1141 tatagaacaa aattgatgca tatatagtat ttatacaaaa tataaatatt atatatttaa
     1201 aattaagtaa aaatattaca taaaggggag atttgagaaa tggaaaaagt caagttgagt
     1261 gttgaaagat gtaagggatg ctatctttgt attgaagctt gtaaaaaaga tgcaataagt
     1321 ctttctgact acacaaataa aaaaggcttt gtaactatta aagtcgacga agaaaaatgt
     1381 attcaatgtg gcgcttgtta tacaatgtgc cctgatttag tatttgagat attataaatt
     1441 atttttatta gaggggagaa aaggtatgca aagtttatta attaaaggaa atgaagcttt
     1501 agcagaaggt gctatacgtg gtggctgcag attttatgca ggttatccaa taacaccaca
     1561 atcagaaata ttagaaatga tggcatggag acaaaaagaa gttggaggag tatttatcca
     1621 aggtgaatca gaaatagcca gtgtaaatat ggttatggga gcaagtgcac ttggagcaag
     1681 agctatgact tcttcatctg gtccaggatt ttcattaaaa caagaaggta ttgcatattg
     1741 ggtatcagct ggtttgccag cagttgtagc atgtgtgcaa agatatggta taggtgatgg
     1801 atttatagga gctggtcaag ataattattg gcaagcagta aaaggcggtg gaaatggaga
     1861 ttatcattta atcgtgttag cacctaatag tgttcaagaa aatatggata tggcatatga
     1921 atcttttgaa ttggctgaaa aatatatgca tccagtactt atactttctg atgctacaat
     1981 aggtcaaatg gtagaacctt gtatagcacc accaatgaaa gaatacgata tggataaagc
     2041 tccatgggct gcaaaaggaa cacctgtagg agaaaaaaat aaagtaataa ctgatataac
     2101 atattttgaa gaccaagata catggcaaaa agggtatata gaaaaaatga gaaaagttca
     2161 agaagatgaa caaagatggg aagagataga aatagaagat gctgaagtgg tatttgtggc
     2221 atacggtatt tcatcaagaa tagcagaagg tgcagttaga gatgctagag caaaaggaat
     2281 gaaattaggt ctaataagac caaaaacatt gtggccttac ccaagaaaag catttaagaa
     2341 attaagtgat tcattgaagg gacttgtaac agttgaaatg aacatgatgg cacagatgaa
     2401 ggaagatgta attatagcat gtgataatag atttccagta tattcagtag caacagctca
     2461 atatatggca gaagtaaatc aattaataga ttttgctcaa cagattatag atggaaaagc
     2521 taagcaagag gaggtatttt aattatggct aaaaaagcac cagcaataat gatgtgtgaa
     2581 aatatgttct gtggaggctg tggacactct atagtcgata gaattattgg agaagtatta
     2641 acagaaatgg atatatgtca agatacaatt atatgttcag atataggttg taatcatatg
     2701 tttcaattta gtatgaatgt agatgttgtc gttccacctc atggtaaaat gggagcagca
     2761 atggcagcta tgaaaagagt tagaccagat aaatatatat ttacaatagc tggagatggt
     2821 ggagcttatg caatagggct tggagaaact atgtcagctg ctactagaaa tgataatgta
     2881 atatttcttg ttatgaacaa taatatgttt gcaatgacag gagggcaaat ggctccaact
     2941 actatagaag gtcaatatac agcttctact ccagcgggta gaaattacgc agatcatggt
     3001 tcaacatttg atgttgtaaa agttatggga aatttagata tagcatatct agcaagagga
     3061 acaataacta accctaaaga aataaataca acaaaaagat acatcaaaaa agctctagaa
     3121 aaacaaaaag aaaagaaagg tttttgctta atagaagtgc ttgtaccatg tccaacaaac
     3181 tggggattaa agccagtaga tgctatgaag agaattgaaa atgaacttgt tccaaagtat
     3241 ccacttggag aatttattga ttagggagga aataatatgt tgaatgaaat tatatgtgct
     3301 ggttttggag gacagggagt tcttacagca ggaaaaatta taatgtatgt tgcatataag
     3361 gcagaaaaga aaattacatg gttcccatct tatggaaatg agatgagggg aggaagtgca
     3421 aattgcaatg ttgttgtaag tgatgaaaaa attgcaaatc cttgtgtaaa aaatccagat
     3481 atactattgg gaatgaacag cgtatcagta gataagtatg aaagtttaat gaaaaaaaat
     3541 ggttatatgt ttgtcaatga atcactagta agtaaggata gaaagtacag agaagacatt
     3601 aatgttataa aagtacctgt tactgaaata gcacaaaatt taggcaatga aaaagctgct
     3661 aacatttgca tgttaggtgc tatggtaaag agtttggatt tatttacaaa agaagaattt
     3721 gaaagtggta tgtgtgaata ctttgaaaat caaggcaaag gtaaattcaa ttctaaaaat
     3781 gtagaagcat ttgaagctgg atacgactac gaatgaatat tttaaatatt taaaataatt
     3841 attgaaatac ttatttttat atgttagtat ttaattaata aaatatagaa cgtcgttctg
     3901 gacaatagga cgaagtcttt aggtcggaat acaagaacgg cgttctgcat agtagaacta
     3961 gggggtgtta aattgaaagt tttagtaata aatcctggtg gtacatccac aaaaatttct
     4021 gttttccaag atgaaaatga aattttgaaa aaaaatataa cccatacaag agaagattta
     4081 aaaaattttt ctaaagtatt tgatgaatat gattatagaa aacaactgat agtagatatt
     4141 ttaagtagtg agaatcatag tataaattct tttgacgcag tagttggtcg tggtgggctt
     4201 atgaaggcta taaaaggtgg tacctatact gtgagtgaag aaatgattga ggatatgaga
     4261 aatgaaataa atggggaaca tgcatcaaat ttaggtgcat tattagctaa aactatagct
     4321 gatgaaatag gagtacaatc atttgtagta gacccagttt ctgtagatga atttgatgat
     4381 gtatcgagga ttacaggaat atcagacata gaaaaagcaa gttggttaca tgcattaaac
     4441 cataaagctg tttcaagaaa aattgcacaa aaactagggg gaaaatacga ggattataac
     4501 tttattgtag ctcatttagg ttcaggaata tcgattgtag cacataaaca aggcaaaatg
     4561 attgatggaa gtggtggtag gtctgatggt ccattttcgc cagaaaggag tggtggatta
     4621 cttgcatatc ctcttataga attgtgttac tccggaaaat atactcatga tgaaatggtc
     4681 gcaaaagtaa gttcgattgg gggaatgtac gattatttag gaacaaagga catgatagaa
     4741 attgaaaaca gaatcaagga gggtgatgaa aaggcaaaac ttataatgga tgcatttatt
     4801 tatcaagtag caaaagaaat ttctatgtat ggagcttcac tttcagggaa tgtagataga
     4861 gttatcttga caggagggat atctcactca gaaatggtag tcaaaggagt agcagaaaaa
     4921 gtttcatatc tagctccaat agagatagta ccaggagaga tggaaatgga ggcacttgct
     4981 ctaggggcac ttagagtact taatggagaa gaaatagcaa aaacttatta atatttagga
     5041 gaaagagtat gttaaaagat tttaataatt tattagaaga ggctaaaaag cttgaacctg
     5101 ttattgtaag tgttgctgtt gcagaagaca aagaagtatt aaaggctgtg aaatcagcaa
     5161 cagatttaaa cattataaag ccaattcttg taggagacaa ggataaaata gaaagtatat
     5221 caagagaaat tggattaaaa ggtcatgaaa taataggttg tcaaaatccc gatgaatcag
     5281 ttaaaaaagc tgtagagata gttaaaaata aggaagctaa agttcttatg aagggattag
     5341 taaatacaag tgtctatatg agagctatat taaataaaga aacgggtctt agaacaggaa
     5401 gattattaag tttgcttgca gtatatgaat tacctcaata tcataaactt ctttattgta
     5461 ctgatagtgg aataaatgta tcgccaaact taaatcaaaa aaaagatata atgacaaatg
     5521 cacttcttac tatgaaaagt ataggcatgg aaaatcctaa aactgcgata cttactgcaa
     5581 atgaaatggt agaccctaag atacaatcta cagtggatgc caacctactt gtagagatgg
     5641 aaaaatcggg agaaatacca aagtgtatag tggaaggtcc aatagcattt gatgtagcat
     5701 ttgatgcaca tgcagcagaa cataaaggca ttaaaagtaa tattgctggt gatgttgatt
     5761 tattagtatt tccaaatata gaagcaggga atatattagg aaagtcatgg cttagattta
     5821 ataaagctaa gtgggcaggt atagttttag gagcaacaaa tccgataatt ttaggttcac
     5881 gttctgacac agctgaaata aaaataaatt caatagcatt agcgtgccta gcatcacaat
     5941 aagaaattat tattaaggag gaaaaagtat tatgaaatat gcaaaaagaa tggatatggt
     6001 aaaagcttca gctataagag attctcaaaa gaaaatagct cagaaggtag caagtggtgg
     6061 aactgttata tcttttgctg cagggcttcc tgacccaaat ttatttccaa tagaagagat
     6121 aagtgaaata actcaaaaag tacttaatga aaggggaaaa tttgccctag catatggacc
     6181 tactaaaggt gaagatgagt tgttagatct attagtaaaa agaatgaaag aagaagaaaa
     6241 tattgataca aaagcagaaa atattataat aactactggg tcacaacaag ggatagcatt
     6301 aagtgctatg attttattag aaaagggaga tgtagtagtt acagaaaatc caagttactt
     6361 aggtgcaata aatgcattta gaccttatga atgtgatttt ttaggagtag atacagatga
     6421 agaaggaatg gtaacagatg atttagacaa attattatct gaaaattcta acataaaggt
     6481 gatatatgta ataccaacat ttcaaaaccc tacaggaaaa acatggtctt tacaaagaag
     6541 aaaagatttt atggaagtcg tgaataaata tgatgtcata gtaatagagg ataatcctta
     6601 tggagaaata agatttacac cagaacctct acctacactt aaatctcttg atactaaaga
     6661 taaagtattg taccttgggt cattttcaaa ggtattatgt ccaggattta gggttgcttg
     6721 gatgtgtggt aatgtagaca tcatagataa ggcagaactt ttaaaacaag gtatagattt
     6781 acagtcaaat caattttcgc aagtacaagt aatagaattt ttaaagaaat acaacttaaa
     6841 tgaacatata gaaaaaataa gagcagagta taagaaaaga tgtctgctaa tgttagatgc
     6901 tatgaagaaa tatttcccag aggaagctaa atatacagaa ccagatggag gaatgtttat
     6961 atgggttgaa cttcctgaaa gtataaatgt agatgattta ttagataaag ccattgatgc
     7021 tggagttgct tatgtttctg gagagtcctt ctttgcaaat aatggtccaa aaaatactat
     7081 gagacttaac tatacaacaa tgtcagaaga acaaatcgtt aaaggtgttg agatacttgc
     7141 agaagttata aaaaaagaac tcaattatgc ttgtgaataa atataaaaag tgagggttta
     7201 aatatgggag aaaaagaaaa cggtataaag tttggaaagt taatgtttca acttgttatt
     7261 atagcaatat cttgggaatt agtttatatt ataccattta tccaatatac attatatgat
     7321 ccaatcctaa aagcattaga atgtacaaac actcaactag gttttctact tacaatttat
     7381 gggttaggta atatatttgg agctcctata ggaggctggt tagcagatag atttgattat
     7441 agaaagataa ttttaggttc tgtatttctt aatggagtag tatcattttt atttgcattt
     7501 aatatgaact atccatttgc agttgctact tgggtaggtt gtgcaatcac atcattagtt
     7561 atgaactatc cttctatggt aaaaatactt agagttatag gaaaagataa tcaaggtaag
     7621 gtttatggat ttaatgaagc tatggttgga gtttctggag tagtaatggg agcaatattc
     7681 ttatatattt atacatgctt tgcaactcct acattgggta tgagatgggt aatgatatct
     7741 ttaggaatat tatcaatagc aatgtgtcca gtattatggt ttgtaattaa agatgtagat
     7801 attaaagaag aaagagaaga agcaggtgaa aaaatgtctg ctggtgattt tataacagta
     7861 ctaaaatcac caaatacttg gctagtagga ataagtatat tctgtgtata ttcattcaca
     7921 gttacaatgt cttattttac accatatata acatctgtac ttggaggttc tgtagcatta
     7981 tctggtgcat tagcaataat aagacagcat ggtctaaaat tatttggagc accatttgga
     8041 ggatattgtg cagacaaatt aaaatctcct acaaaagtac ttttaccaat atatgtattt
     8101 ggtatagcag taatagtttt attcctagta ttacctgcat caactccaat gactatattc
     8161 atagctttaa catttgtagt aggtatatta ggttacatgg gaaaaggtat atattatgca
     8221 gtacaagatg aagtaaaagt tccagttaaa tattcagcaa ctactatagg tatagctgca
     8281 gctttaggat tttcaccaga tgtattccaa tttgcattaa taggacactg gatagatact
     8341 tatggcaata aaggttacac atatacattt atattccaaa tagcaattct agttattgga
     8401 atactatctt gtttatatat attaaaggtt aaaaagagaa gagcaagtaa attagaagct
     8461 taaatattca atttggtaat taagtatata taaatacaat acttattttg atgaatgtga
     8521 ttgtataaat aacataaaaa atatcaatag attaagtttt ataataatgt ttagagaata
     8581 aagtataaaa ataaaaatgt tatcaagttt tctaggtttt aacataaagt tacccctgaa
     8641 ggtgtaaaat tgagtggcaa ttattatcta ttttcttacc tacataaacc tatttaattt
     8701 aaattctaag agattttcaa aatagatggt agttgtactc aattttaaac ctcataataa
     8761 atacga
//
LOCUS       DBGJFF010000148         8404 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.151, whole genome shotgun sequence.
ACCESSION   DBGJFF010000148 DBGJFF010000000
VERSION     DBGJFF010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8404
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.151"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            111..563
                     /locus_tag="ACOIAF_003333"
     CDS             111..563
                     /locus_tag="ACOIAF_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGZ2670326.1"
                     /translation="MVLQEKYIFIDKDLKSKEEVLNFIADSAYILGITTNKEALLKDL
                     LKRENEFPTGIKDGFAIPHTKSVNVKEASILYIKTTNELEWKTLDDSKVRYVFSLLAP
                     HKNEGNIHLKTLSKLATCLMEDDFTSEVKNASDASHLVKYITNKMKEE"
     gene            566..874
                     /locus_tag="ACOIAF_003334"
     CDS             566..874
                     /locus_tag="ACOIAF_003334"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HGZ2670327.1"
                     /translation="MKIVAITSCIAGLAHTPMAAKALEQAASKMGHEIKVEQQGAMGR
                     VNTITKEEAQDADFILIASDQTISGMERFDGKKVIKVKIGIALKKPEAVLTKCIEAIS
                     "
     gene            988..2094
                     /locus_tag="ACOIAF_003335"
     CDS             988..2094
                     /locus_tag="ACOIAF_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HGZ2670328.1"
                     /translation="MSKKNKSLLSELKGHLMTGISYMLPLVIGSSLVVAIPKLTALAV
                     GITSLDAYKNVSGFYHILYLMEQVGWTGIGLLNTVLAGFMAFSIGEKPAMGAGFIGGL
                     IASNTNAGFLGAVIAGFFAGYVCKFLKKKIKISGSASGMMPLIIMPLITVGLTGFLMS
                     VVLAGPLGNINTSLNAWVAEMCQNGTNSVVLALILGAMIGFDLGGPVNKAAWMAGNAL
                     LLEGIYLPAILVNCAIVIPPLGYGLATFIRKSKFSNALAETGKGNIVMGIIGITEGAI
                     PFTLTNPLKLVPVNMIGCALGVGLSALLGVHAIMPPVGGLYGFISIGSGWAYLVGAIF
                     GALVIAILSTTLVDFNEDETTENTEFDEIELDIM"
     gene            2169..4847
                     /gene="mngB"
                     /locus_tag="ACOIAF_003336"
     CDS             2169..4847
                     /gene="mngB"
                     /locus_tag="ACOIAF_003336"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HGZ2670329.1"
                     /translation="MKKTKVHVVPHSHWDREWYFTTSRSKIYLMKDFQDILDILEEKE
                     DFKYFTMDAQASLLDDYLKWRPQDEYRIRDLVQRRRLVIGPWYTQTDQLVISGESIIR
                     NLYYGIDRCNEFGEPMRVGYVPDSFGQSAQMPQIYRGFGIDSSLFWRGVSDDMVDTTE
                     FIWKGSDGSKVLAVQIPFGYYYGGNIPEDDSDLKEYLKDIIVKLKVKASTNNVYFPNG
                     FDQAPIRKNLPDILKKANEIDNENEYEISDIENYIDSVRNERNEFTELKGEFLNAKHM
                     RIHKSIFSSRSDLKIMNNKLENYITNVLEPLLTLSYSLGNEYPHLVIKDIWKLMFENA
                     AHDSIGSCNSDTTNEDVYTRYKQARDLSINLLDLHMRLISTQIKNNNEEITLTVFNGF
                     PEVRNEVVEFDTYIPGQDFVIKNKNGKILNYIIKEKEDITNYILTQTIRLNPSKKIYI
                     PKTVYRAKIALSVKDIPPMGYTQLVFELDTKNKCEIETSNCKEIENKYYKITANKNGS
                     LEILDKESNKTYKNQAVIEDNGDDGDSYNYSPPRKDIYVSSLDSVSSVEVLKSNIQEE
                     MILKYSLTIPTSLEERAIGKVSVKMPVTMKITLEPKEQIIKFNVNIKNNQALSHRVRV
                     LFNTEIASKFSIADQQFGIIQRPTVLEKDLALWKRDNYSWQEKPITIEPMQSFVTLED
                     GQRGIALITGCVREYQIVGDNLDTIALTLFRSFGYMGRENLLYRPGRASGEKIIATPD
                     AQLLKELNFDFGLYIYNNKFDEANVANTAKRFLTPIQIYEYADFLNGRLIFAFNDEKQ
                     IYENEFSLMNVNNSNFTVSAIKKEEKGDGIVVRIFNGMKNEDAKGSLNINKNVNDAYS
                     AMLDEIYDNTSKLKVNTKTVEVNSLSHCKVQTIVIK"
     gene            complement(5164..7500)
                     /locus_tag="ACOIAF_003337"
     CDS             complement(5164..7500)
                     /locus_tag="ACOIAF_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HGZ2670330.1"
                     /translation="MLDTKLKKFKDKYIVTIIVFILMLSASLGMISQYSTIQAGAKGG
                     AKNPFEQESFVDSIYKGSYVLYHNMKEEQEGKMIQPSKLFINEETMNYIKNVSKEKDT
                     YDGSETYYTDDSINADFNENFENWEHFIKASGKNLKYYLVDKESNFEFFNDNKELGVL
                     DTTKNDASKENSDENQSKNTEMNDEERQKQITDIKENYRFYVVMNFDKDGKVNIVDSY
                     GVNQKVINQMLLSKAREDLMDTSLGGNSISYKLSPIKNITLVYAVPKAISKGDSVEYG
                     YGNTYFDDGIISYISNVENNSYYKISGMILKIILAIVVIVALAFPYRKSKELLGFDTL
                     SRIPLEILFIALAIIQGLVDLYPTVIISNTLSGNYVEYLINNDISAKMASILVSLMNL
                     TYWFVIFSIIFVFIILLKHILNVGIREYFTKHTVTGMFFVWLKKAGKTVINQIRTINL
                     KEKPNKTIVTILGINLLIIVIMCSMWFFGIILALIYSVVLFKILSDYSKKTIYEYNQL
                     LDVTKKISDGNLDVNMEKDLGFFNPIKDELGNIQTGFKKAVDEEVKSQKMKTELISNV
                     SHDLKTPLTSIITYIDLLKDESITDENRKLYIDTLDRKSQRLQHLIEDLFEVSKANSG
                     DVYLNIVNVDIVSLMKQTLLELDDKLADSSLIVKNNFSNEKIILPLDSQRTFRVFENL
                     IINISKYAMPNSRVYIDILETDNKVNIMLKNMSASEIDFSVDDIMERFVRGDKSRNTE
                     GSGLGLAIAKSFIELQGGKMNISVDGDLFKVTITFNKN"
     gene            complement(7472..8188)
                     /locus_tag="ACOIAF_003338"
     CDS             complement(7472..8188)
                     /locus_tag="ACOIAF_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGZ2670331.1"
                     /translation="MNSYNILVVEDEKEIADAIEIYLLNQGYNVFKGYNGLEGLKVIE
                     NQEIHLAIVDIMMPKMDGITLTMKLRENYNFPVIMLSAKSEEVDKIMGLNIGADDYVT
                     KPFKPLELLARVNSQLRRYTKYLNMVGNKEQKVESDDSVFAIGGLELNENTKEVSIDG
                     KYIKTTPIEFKILSLLMRNAGRVFSADEIYERVWNENAVNTDTVMVHVRNIREKIEID
                     PKNPKYLKVVWGVGYKIEKI"
BASE COUNT         3142 a         1177 c         1161 g         2924 t
ORIGIN      
        1 ataatataaa tttccatgtc gaagtggaaa ttagttatat ttaatttaat taggataatg
       61 attacaatat aaatataagt taagacatta tgaatggagt ggaaaaatat atggtactac
      121 aagaaaaata tatatttata gataaagact taaaaagcaa agaagaagta ttaaatttta
      181 ttgcagatag tgcttatata ctaggtatta caactaataa agaggctctt ttaaaagacc
      241 ttctaaaaag agaaaatgaa tttccaacag gaataaaaga tggatttgcg atacctcata
      301 caaaaagtgt aaatgtaaag gaagcttcca tactctatat taaaacaact aacgagttag
      361 agtggaaaac tttagatgat agtaaggtta gatatgtatt tagtttgtta gccccacata
      421 aaaatgaagg aaatatacat ttgaaaactc tatctaaatt agccacatgt ttaatggagg
      481 atgattttac aagtgaagtt aaaaatgcta gtgatgcaag tcatttagta aaatatataa
      541 ctaataaaat gaaggaggaa taattatgaa aatagtagca ataacatcat gtatagcagg
      601 attagcacac acacctatgg cagcaaaagc tttagaacaa gctgcatcaa aaatgggtca
      661 tgaaatcaaa gtagaacaac aaggtgctat gggacgagta aatactataa caaaagaaga
      721 agcacaagat gcggacttta tacttatagc atctgaccaa actatatctg gaatggaaag
      781 atttgatggt aaaaaagtga taaaagtcaa aataggtata gcgttaaaaa aaccagaagc
      841 tgtcttaaca aagtgtatag aagctatttc ttaataaaaa aatctcactc taatatagaa
      901 tgagaaaaat caaaaattaa aaaattgtct aatgtaatta taacatatta tcaaaaaata
      961 taacaaactt atataggagg attaaaaatg agtaaaaaaa ataaatctct tttaagtgaa
     1021 cttaagggac acttaatgac aggtatctca tatatgttgc cacttgtaat aggctcatct
     1081 ttagttgtag ctatcccaaa gcttacagct ttagcagtgg gtataacaag tttagatgct
     1141 tataaaaatg tatctggttt ttatcatatt ctttacttaa tggaacaagt tggttggact
     1201 ggcataggtc tattaaatac ggtattagca ggatttatgg cattttcaat aggtgagaaa
     1261 ccagctatgg gtgcaggttt tattggtggg ctcatagcaa gtaatactaa tgcaggattc
     1321 ttaggagcag taatagcagg tttctttgct gggtatgttt gtaagttttt aaagaaaaaa
     1381 attaaaatat ctgggtcagc atcaggaatg atgccactta taataatgcc acttataaca
     1441 gttggattaa ctggtttttt aatgagtgtt gtattagcag gacctttagg aaacataaat
     1501 acttcattaa atgcttgggt agctgaaatg tgtcaaaatg gaacaaattc ggtggtattg
     1561 gctttaattt taggcgcaat gataggattc gatttaggtg gaccagtaaa taaagcagct
     1621 tggatggcag gaaatgcatt attgttagag ggaatctatc taccagcaat attggtaaac
     1681 tgcgcaatag ttatacctcc actaggatat ggtttagcta cattcataag aaaatcaaag
     1741 ttttccaatg cattagctga aacaggaaaa ggcaatatag ttatgggtat aattggaata
     1801 acagaaggtg caattccttt cactttaaca aatccattaa aattagttcc tgttaatatg
     1861 ataggatgcg ctttgggtgt aggattatca gctttgttag gtgtacatgc tataatgcct
     1921 ccagttggtg gattatatgg atttatatca ataggttctg gatgggctta cttagtaggt
     1981 gcaatatttg gagctttagt aatagcaata ttatcaacaa ctttagtaga cttcaatgaa
     2041 gatgaaacta cggaaaatac agaatttgat gaaatagagt tagatataat gtaaattaaa
     2101 aacttaattt tagataattg agaattgagt gtactctcaa ttctcaatta aattttggga
     2161 ggatttttat gaaaaagaca aaagtacatg tagttccaca cagtcattgg gatagagaat
     2221 ggtattttac aacatcaagg tcaaaaatat atttaatgaa agattttcaa gatatattag
     2281 acatattgga agaaaaagaa gactttaaat actttactat ggatgcacaa gcttctctct
     2341 tagatgatta tttaaaatgg agacctcagg atgaatatag aataagagat ttagttcaaa
     2401 gaagaagatt agttattgga ccttggtata ctcaaacaga ccaattggtt atatcaggag
     2461 aatctataat tagaaacctt tactatggaa tagatagatg caatgaattt ggtgaaccta
     2521 tgagggttgg atatgtacca gattcttttg ggcaatcagc tcaaatgcca caaatataca
     2581 gaggttttgg aatagatagt tcattatttt ggcgtggagt ttctgatgat atggtagata
     2641 caacagagtt tatatggaaa ggttctgatg gttcaaaagt attagcagtt caaataccat
     2701 ttggttatta ctatggaggt aatataccag aagatgattc agatttaaaa gaatatttaa
     2761 aagatataat cgtaaaactt aaggttaaag cttctactaa taacgtatat tttccaaatg
     2821 gatttgacca agcaccaata agaaaaaacc ttcctgatat attaaaaaaa gctaatgaaa
     2881 tagataatga aaatgaatac gaaataagtg atatagaaaa ctatatagat tcagtaagaa
     2941 atgaaagaaa tgaatttaca gagcttaaag gtgaattttt aaatgctaaa catatgagaa
     3001 tacacaaatc catattctcc tcaaggtcag accttaaaat aatgaataac aaattagaaa
     3061 attatattac aaatgtactt gaaccattat taacattaag ttattcactt ggaaatgaat
     3121 accctcacct agttataaaa gatatatgga aacttatgtt tgaaaatgca gctcatgaca
     3181 gcataggttc ttgtaatagt gacacaacaa atgaggatgt atacacaaga tacaaacaag
     3241 ccagagattt gagtataaat ttattagatt tgcatatgag acttataagt actcaaatca
     3301 aaaataataa tgaagaaata acattaacag tattcaatgg attcccagaa gtcagaaatg
     3361 aagttgtaga atttgatact tatattccag gtcaagattt tgtgataaag aataaaaatg
     3421 gaaaaattct taattatata ataaaagaaa aagaagatat tacaaattat atattaactc
     3481 aaacaataag attaaaccca agtaaaaaaa tatatatacc caaaactgta tatagagcaa
     3541 aaattgcact ttcagtaaaa gatataccac caatgggata tactcaacta gtttttgaat
     3601 tggatactaa aaataaatgt gaaatagaaa caagtaattg caaggaaata gaaaacaaat
     3661 actacaaaat aacagccaat aaaaatggtt cattagaaat attagataaa gaatctaata
     3721 agacttataa aaaccaagct gtaattgaag acaatggaga tgatggagat tcatacaact
     3781 attctcctcc aagaaaagat atttatgttt catctttaga ttctgtatct tcagtagaag
     3841 tgctaaaatc aaacattcaa gaagaaatga tacttaaata ttcattgacc ataccaactt
     3901 cactggaaga aagggctata ggtaaagttt ctgtgaaaat gccagtaaca atgaaaatca
     3961 ccttagaacc taaagaacaa ataattaagt ttaatgttaa tataaaaaat aaccaagcac
     4021 tatctcatag agtaagagtt ttatttaata cagaaatagc atctaagttt tctatagcag
     4081 atcaacaatt tggaataata caaagaccaa cagtattaga aaaagacctt gcattgtgga
     4141 aaagagacaa ttactcatgg caagaaaaac ctataactat cgaaccaatg caaagttttg
     4201 ttactttaga ggatggacaa agaggaatag cattaataac tggttgtgta agagaatatc
     4261 aaatagttgg tgataattta gatacaatag cattaacact attcaggtca tttggatata
     4321 tgggaagaga aaatttactt tatagaccag gaagagcttc tggagaaaaa ataatagcaa
     4381 caccagatgc acaattactt aaagagttaa actttgactt tggtctttac atatataaca
     4441 ataaatttga tgaagcaaat gtagctaata cagctaagag attcttaaca cctatacaaa
     4501 tatatgaata tgctgatttt ttaaatggta gacttatatt tgcattcaat gacgaaaaac
     4561 aaatatatga aaatgaattt agtttgatga atgtaaataa ttcaaatttc acagtaagtg
     4621 caataaagaa agaagaaaaa ggagatggaa ttgtagttag aatcttcaat ggtatgaaaa
     4681 atgaggatgc taagggaagt cttaacatca ataaaaatgt caatgatgct tatagtgcaa
     4741 tgctcgacga aatctatgat aatactagca agcttaaagt aaatactaaa actgtagagg
     4801 taaattcttt atcacattgt aaagtacaaa caatagtaat taaataatat aaaatagctt
     4861 atacaagatt tcaaaataaa ttgctagtac tatgttaata gtaaagccag atattatgct
     4921 atttataagc tctgtaacac taaataacat tttgtaaaat aaaaaggtga accttgtata
     4981 ttaggattca ccttttttaa ataaatcaac attctgtttt aaaataacat ttataagctg
     5041 ttttgaaata atgcttacaa attattttaa aacaatacct ataaattttt atttaaagaa
     5101 tacacataaa tatttccaga ttcttttatt ctatagactt tataattatt aaagaagtct
     5161 aatctaattt ttgttaaaag taattgtaac tttgaataaa tcaccatcca cacttatatt
     5221 catcttacca ccctgtaatt ctataaaact ttttgctatc gctaaaccaa gaccagaacc
     5281 ttctgtgtta cgtgatttat ctcctcttac aaatctctcc attatatcat ctacactaaa
     5341 atcaatctca gatgcagaca tatttttaag cataatattt actttattat ctgtttctaa
     5401 aatatcaata tacactcttg aatttggcat tgcatatttg cttatattta ttataagatt
     5461 ttcaaatact ctaaatgtac gctgactatc taatggtaat attatttttt cattagaaaa
     5521 gttattcttt actattagag atgaatctgc aagtttgtca tctaattcta aaagtgtctg
     5581 cttcattaga gatacaatat ccacatttac tatattcaaa taaacatctc cactatttgc
     5641 cttgcttact tcaaacaaat cttctattag atgttgcaat ctttgtgact ttctgtctaa
     5701 tgtatcaata tacaacttac gattttcatc tgttatactt tcatctttta ataaatctat
     5761 ataagtgatt atagatgtta gaggtgtttt taagtcatgt gatacatttg atataagctc
     5821 tgttttcatc ttttgactct taacctcttc gtcaacagct tttttaaatc cagtttgaat
     5881 atttcctaat tcgtctttta taggattaaa aaatcctaaa tctttttcca tatttacatc
     5941 taagtttcca tctgaaattt tctttgtaac atctaaaagt tgattatatt catatattgt
     6001 ctttttagag taatctgaca gtatcttaaa taatactact gaatatatta atgcgagtat
     6061 tattccaaag aaccacatac tacacattat aactataatc aataaattta ttcccaaaat
     6121 tgtaactata gttttatttg gcttttcttt tagatttatt gttctaattt gattaataac
     6181 tgtttttcct gcttttttta gccatacaaa aaacattcct gtcacagtat gttttgtaaa
     6241 atactctctt attccaacat ttaaaatatg ttttaaaagt attataaata caaatattat
     6301 tgagaatatt acaaaccaat aagttaaatt cattaaactt accaatatgc tagccatttt
     6361 agcacttatg tcattgttta taaggtactc tacataattc ccactcaagg tattacttat
     6421 tataacagta ggatataaat ctacaagccc ctgtataata gcaagagcaa taaagagtat
     6481 ctctaacggt attcttgata atgtatcaaa tcctagcaac tctttagatt ttctatatgg
     6541 gaatgctaaa gctacaataa caactatagc taatataatt tttaaaatca ttccagaaat
     6601 cttataataa gaattatttt ctacattgct tatatatgaa attataccat catcaaaata
     6661 agtatttcca taaccatact ctacactatc acctttactt attgctttag gtacagcata
     6721 tactaatgtt atatttttta ttggagataa cttatatgaa attgaatttc ctcctaaact
     6781 tgtatccatt aaatcttctc ttgctttaga taacaacatt tgattaataa ctttttgatt
     6841 tactccataa gaatccacaa tatttacttt tccatcttta tcaaaattca ttacaacgta
     6901 gaatctatag ttttctttta tatctgtaat ttgcttttgt ctttcctcat cattcatttc
     6961 tgtatttttt gactgatttt catcagaatt ttcttttgaa gcatcattct ttgttgtatc
     7021 aagtactcct aattctttat tatcattaaa aaattcaaaa ttgctttctt tatcaactaa
     7081 atagtacttt aaattctttc ctgacgcttt aatgaaatgc tcccaatttt caaagttttc
     7141 attaaaatct gcattaatac tatcatctgt ataataagtt tctgaaccat cataagtatc
     7201 tttttcttta cttacatttt ttatatagtt catagtttct tcattgataa atagctttga
     7261 tggttgtatc atcttacctt cttgttcttc cttcatatta tgatataaaa cataacttcc
     7321 tttatatata ctatctacaa agctttcctg ttcaaatgga ttctttgctc cacctttagc
     7381 acctgcttgt atagtagaat attgactaat catacctaat gatgctgata acataagtat
     7441 aaaaactata atagtaacta tatatttatc tttaaatttt ttcaattttg tatccaacac
     7501 cccacaccac ctttaaatat ttaggatttt ttgggtcaat ttctatcttt tcccttatat
     7561 tcctcacatg taccataact gtatctgtat ttacagcatt ctcattccaa actctttcat
     7621 atatttcatc agcagaaaaa actcttcctg catttctcat aagtaaactt agtattttaa
     7681 attctatagg agttgttttt atatactttc catcaatact aacttctttt gtattttcat
     7741 ttaattctaa tccacctatt gcaaacacac tatcgtcact ttctaccttt tgctctttat
     7801 ttccaaccat gtttagatac ttagtgtacc ttctaagttg tgaattaact ctagccaata
     7861 gttctaatgg cttaaaaggt tttgttacat aatcgtctgc tcctatatta agccccatta
     7921 ttttatcaac ttcttctgat tttgcagata gcattattac tggaaaatta tagttttctc
     7981 taagcttcat tgtaagtgtt ataccatcca ttttaggcat cattatatca actatagcta
     8041 aatgtatttc ttggttttct ataaccttaa gaccttctaa tccattgtat cccttaaaaa
     8101 cattatatcc ttgatttaat agatatattt ctatagcatc tgctatctcc ttttcatctt
     8161 ctaccaccaa aatattatat gaattcattc taatctcctt tatattaatt tataaatttt
     8221 ctttacatat atgattatac aatatgatag aagtttttcc atttatatat atatttatat
     8281 cttttgttct ggttatttgt atttaatgtc catcttagaa acctccttct agtttaaatt
     8341 taatattttt tcattttgtt ttatatactt aatattaaaa ttaaaaactt aagatattac
     8401 atat
//
LOCUS       DBGJFF010000149         8385 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.152, whole genome shotgun sequence.
ACCESSION   DBGJFF010000149 DBGJFF010000000
VERSION     DBGJFF010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8385
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.152"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            240..1226
                     /locus_tag="ACOIAF_003339"
     CDS             240..1226
                     /locus_tag="ACOIAF_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGZ2670332.1"
                     /translation="MKKITINDIANLAGVSKSTVSRYLNNKDISDSTKEKIKTIIDEY
                     GYEPNAFAQSLRAKKTYFIGIITPCLDSFVKSKIMMAIDEELKELKYTSLIINTNRKI
                     RSEIDSISKLASLKVDGIILIGTEITKEHKNEIEKLDIPIVVVGQKVDGINSIVNDDY
                     GAGYKMGQYIANKGYKNIVYLGVDESDISVGLNRKNGVLNGLKDKGYDAKVFYTDFDQ
                     ETSIQRSGEMLESENPDIIICATDNIAIATMKEINKRGKSIPQDISVAGFGGYDVLSI
                     ISPKLTTIKFENKNAGKVAANTIVNLIQERKEPLLKEIKFELIEGESTINKN"
     gene            1467..1544
                     /locus_tag="ACOIAF_003340"
     CDS             1467..1544
                     /locus_tag="ACOIAF_003340"
                     /inference="COORDINATES: protein motif:HMM:NF012587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
                     /protein_id="HGZ2670333.1"
                     /translation="MKGVAHCVTRLRIVLDSNNKANIKL"
     gene            1563..3044
                     /locus_tag="ACOIAF_003341"
     CDS             1563..3044
                     /locus_tag="ACOIAF_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HGZ2670334.1"
                     /translation="MYKEPKYRTILESTKEELNKLRNISLNDKYKPLFHIHPQHGLLN
                     DPNGLAYYNGKYHVFYQWYPYDATHGMKHWAYVSSDDFVNWNREDVALIPIESYESHG
                     AYSGNAIEVDGKLHMYYTGNIKYSAEDRYAYQNLAIMNKDGKITKYENNPIVSEIPKG
                     YTGHVRDPKVFKRKDKYFMLLGAQTSDKKGVIIVYESKNSIDWNFKGELNVKNIDEDF
                     GYMWECPDYINIDEKDILIFSPQGVEPKGFDYQNIYNVVYAIGNMDLDNLTFEIDTMK
                     ELEKGFDFYAPQTFIKDSQIILFAWAGMGEVLYPTDKNKWAHCLTVPRKLNIKNNKLL
                     QMPVDELIKLRYDETSGQNTIKNNINIIENNENLYELNINIKNIDSNKFGLELFSSQD
                     EGVKLEFNKLGNIVTLDRSNFKKVFGVEYGTNRKEYINIDENTNIKVLADRSILEIFI
                     NDGEVVFTSRIFAKENSNQIRVYSDKIVEYEYTKFKLKQGIEL"
     gene            3070..4041
                     /locus_tag="ACOIAF_003342"
     CDS             3070..4041
                     /locus_tag="ACOIAF_003342"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HGZ2670335.1"
                     /translation="MKKVISIGEALIDFIPNQNGGKLKNVSEFRRVAGGAPANVSAVV
                     AKLGGKSSFISKLGKDAFGDYIIDVLNEVNVNTDYVLRTSKANTGLAFVSLKEDGNRD
                     FSFYRNPSADMLLEADEVKKEWFNNCHILHFCSVDLIDSPMKLAHKKAIEYALESNSI
                     ISFDPNIRLPLWDSEQSCKKAISEFLPFAHIVKISDEELEFITGENDIEKSLHKLFVG
                     NVELVLYTKGKDGVDAYTKKVKGMCKGVKVNAIDTTGAGDSYIGSFLYTLLYKQITLN
                     DIKEMKQETLNEYLEFSNYYAARSTTIKGAISSYATKEEITEFIKNL"
     gene            complement(4282..4884)
                     /locus_tag="ACOIAF_003343"
     CDS             complement(4282..4884)
                     /locus_tag="ACOIAF_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HGZ2670336.1"
                     /translation="MEVCILMGSPKKKGNTAAILKPFIEELENYNSNIEVIWLYDCKI
                     EPCIACKKCQDNLFDFGCYCKDDVQKIFKKILMCDLIVFATPIYSWYCTSPMKALLDR
                     LVYGMNKYYGNEKGPALWAGKPVALITTCGYRPEKGADIWENGMKRYCKHSQLKYIGM
                     LVERDLGKPNFMNKEKEEHSRQFAKQIYYILDNSIEVDNC"
     gene            5165..6376
                     /locus_tag="ACOIAF_003344"
     CDS             5165..6376
                     /locus_tag="ACOIAF_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HGZ2670337.1"
                     /translation="MYSNEDYKFIEENAPCYIYDETNIIEKCKELKNNILGFDFLYSI
                     KTNPYNKIVKSIAEQGFGADASSTNEVMISSKCGVSKDDIYYSSPGKTEIDIETVLDK
                     CIIIADSITEIELINKISKKHNQKVKIGIRINPDFTMDDDSAKSSKFGIDIEDMSSVF
                     KMLSMCDYVSVVGIHIHIKSQVLDFEKLGHYYEKCFFIALNLNKSKYINIEFINFGSG
                     LGVAYDNLRENPLNLKELSKLTQKIAFTNSNTLKSRLLIESGRFIVCKSGNYYVKIVD
                     IKVSRGKKYLIVKNGLNGFMRPIISNLIENITCGVELEGQEPLYTSNHAFSLSVLNNL
                     KEEEYVNVVGNLCTSLDVIANNVKLKKANIGDLISISNAGSYSYSLSPLLFSSYEFPK
                     QYYKRKDGYIE"
     gene            6444..6641
                     /locus_tag="ACOIAF_003345"
     CDS             6444..6641
                     /locus_tag="ACOIAF_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HGZ2670338.1"
                     /translation="MELWKRNLFVCWIGMFFSSIGMSQIAPILPLYIKQLGVTDVSLI
                     QQYSGIIFGCTFVVAAFFSPI"
     gene            6675..7646
                     /locus_tag="ACOIAF_003346"
     CDS             6675..7646
                     /locus_tag="ACOIAF_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGZ2670339.1"
                     /translation="MLLRASIGMGIVIFTMAFVQNVYQLLGLRILQGVFTGYATACTT
                     LIATQTDKNHSGWALGTLATASTTGSLIGPTVGGYIESILGLKSTFIITGGLLFVSFI
                     ISILFVVDNFKPKEIKESISIPKEQLNILPNKFLIASIFVTTFITQLALYSIEPIVTI
                     YISQLTNFASNVALIAGLTFSASGLANLLAAQKLGKMSDKIGPQKVLLISLLWAGIIF
                     IPQAFVKTAWQLMLLRFLLGLSVAGLNPSVNSLLKKIAPEEYVGKIFGYNASAQYIGC
                     SSGAFLGGQISAHLGIRTVFFSTSLLLFMNALWMYKFTGLFIKDKTK"
     gene            7833..8210
                     /locus_tag="ACOIAF_003347"
     CDS             7833..8210
                     /locus_tag="ACOIAF_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HGZ2670340.1"
                     /translation="MTISKIPQAELKVMKFIWEKNDTVTSKETIEAMERKYGWKQTTT
                     LTLLSRLVKKHFLSAEKIDRYTHYTIVVGHKEYLSVETKDFLSNIHDNSLQSLISALH
                     DDEVVDKDMLDLFESHFKNLEEE"
BASE COUNT         3407 a          876 c         1348 g         2754 t
ORIGIN      
        1 tttttatatt ataaataaat tagagtattt atcaattcat agtagataaa attgaaatta
       61 aatataacaa caaaaaatga aaattacaat taatttaatg ttaaagactc aattatttgg
      121 atgtggaatg gatagtacaa gtagtatata aaaagtattg actggacaaa taactatatt
      181 ttattttaaa tatataatat aatgtaataa aatatatcaa atgtagaagg tgataaccta
      241 tgaaaaaaat aacaataaat gatattgcta acttggcagg agtatctaaa agtactgtat
      301 caagatatct aaataataaa gatatcagtg attctactaa agagaaaata aagacaataa
      361 tagatgaata tggatatgag ccgaatgcat ttgctcaaag tttgagagct aaaaaaacat
      421 actttattgg tattataaca ccgtgtttag attcttttgt aaaatctaaa attatgatgg
      481 caatagatga agaactcaaa gaattaaaat atacatcact aattattaat actaatagaa
      541 aaataaggtc agaaatagat agtatatcaa aattagctag tctaaaagtt gatggaataa
      601 tactaattgg taccgaaata actaaagaac ataagaatga aattgaaaag ttagatatac
      661 cgatagttgt agttggacaa aaagttgatg gtatcaatag tatagtgaat gatgattatg
      721 gagcaggata taaaatgggt caatatattg ctaataaagg ttacaaaaac attgtatatt
      781 taggtgttga tgaaagtgat atatcagttg gtttaaatag aaaaaatgga gtacttaatg
      841 ggttaaaaga taaagggtat gatgcaaaag tattttatac tgactttgac caagaaacgt
      901 ctatacaaag aagtggagaa atgctagaaa gtgaaaatcc agatataata atatgtgcaa
      961 ctgataatat agcaatagca acaatgaaag aaatcaataa gagaggtaaa agtataccac
     1021 aagatatatc tgtagctgga tttgggggat atgatgtatt gtctattata agccctaaac
     1081 ttacaacaat aaagtttgaa aataaaaatg caggtaaagt agcagcaaat acgatagtaa
     1141 acttaataca agaaagaaaa gaaccactat taaaggagat taaatttgaa ttaatagaag
     1201 gtgaaagtac aataaataaa aattaagata cacattacat atattagtaa tgtgtatttt
     1261 tttactaaaa aaactataat tctaaaaatt aggaaaactt ttttgaaaga aatattgcat
     1321 ctgaaataaa tttatgctaa tctttaatta aaccggtttc gaaaccggtt taattaaacc
     1381 gatagaatag caaaatgagt gcaaaaataa ctgattgtaa caaagtatca aagtaattat
     1441 tagagtacat aggaaataaa taaaatataa aaggtgtagc acattgtgta acaagattaa
     1501 gaattgtttt agatagcaat aataaagcaa atataaaatt ataaaagaaa aggaataaaa
     1561 atatgtacaa agagcctaaa tatagaacca tattagaatc gacaaaagaa gaattaaata
     1621 aattaagaaa tatatcttta aatgataaat ataaaccact atttcatata catccacaac
     1681 atggactact taatgaccca aatggattag catattataa tggtaagtat catgtatttt
     1741 atcaatggta tccatatgat gcaactcatg gaatgaagca ttgggcttat gtaagttctg
     1801 atgattttgt aaactggaat agagaagatg tagcattaat accaatcgaa agttatgaat
     1861 ctcatggagc atattcaggt aatgcgatag aggtagatgg aaaattgcat atgtattata
     1921 cagggaatat aaaatatagt gcagaagata gatatgcata tcaaaactta gctataatga
     1981 acaaggatgg taaaataact aaatatgaaa ataatccaat agtaagtgaa ataccaaaag
     2041 gatatacagg acatgtaaga gacccaaaag tatttaaaag aaaggataaa tactttatgt
     2101 tacttggagc acaaacaagt gataaaaaag gagttataat agtatatgaa tctaaaaact
     2161 ctatagattg gaattttaaa ggagaattaa atgtaaaaaa catagatgaa gattttggtt
     2221 acatgtggga atgtccagat tatattaata tagatgaaaa agatatatta atattttcac
     2281 cacaaggagt agaacctaaa ggatttgatt atcaaaatat atataatgta gtatatgcta
     2341 ttggtaatat ggatttagat aatttaacat ttgaaataga tacaatgaaa gaactggaaa
     2401 aaggatttga tttctatgct ccacaaacat ttataaagga ctcacaaata atactatttg
     2461 catgggctgg aatgggagaa gttttatacc ctacagataa aaacaaatgg gcacattgtc
     2521 taactgttcc aagaaaatta aatataaaaa ataataagct attgcaaatg cctgtagatg
     2581 aattaattaa attaagatat gatgaaacaa gtgggcaaaa tactataaag aataatatca
     2641 atattataga aaataatgaa aatttatatg agcttaatat taatataaaa aatattgaca
     2701 gcaataaatt tgggctggaa ttattttcat cacaagatga aggtgtaaaa ttagaattta
     2761 acaaattagg aaacattgta actttagaca gaagcaactt taaaaaagtt ttcggtgttg
     2821 aatatggaac aaatagaaaa gaatatataa atattgatga aaatactaat atcaaagtac
     2881 tagcggatag aagtatttta gaaattttta taaatgatgg agaagtagta tttactagta
     2941 gaatatttgc taaagaaaat tctaatcaaa taagggttta tagtgataaa atagtagagt
     3001 atgagtatac taaatttaaa ttaaagcaag gtattgaatt ataaaaacac attataaggg
     3061 gtagaaaaga tgaaaaaagt aattagtata ggagaagctt taatagactt tatacctaat
     3121 caaaatggtg gtaaattaaa aaatgtatca gaatttagaa gagtagcagg aggagctcca
     3181 gcaaatgtta gtgctgttgt agcaaagctt ggaggaaaat caagttttat atctaagtta
     3241 ggaaaagatg catttggtga ttatataata gatgtattaa atgaagtaaa tgtaaatact
     3301 gattatgtat tgagaacaag taaagcaaat acaggacttg cttttgtatc attaaaagaa
     3361 gatggtaatc gtgatttctc attttataga aatccaagtg cagatatgct tttagaagct
     3421 gatgaagtta aaaaagagtg gtttaataat tgtcatatcc tacatttttg ttcagtagat
     3481 ttaatagata gtccaatgaa attagcacat aaaaaagcaa tagaatacgc acttgaaagt
     3541 aatagtataa taagctttga ccctaatata agattacctt tatgggatag tgagcaatct
     3601 tgtaaaaagg caatatcaga atttttacct tttgctcata ttgttaagat atctgatgaa
     3661 gaattagaat ttataacagg agaaaatgat atagaaaaat cattacataa actctttgtg
     3721 gggaatgttg aactagtatt atatacaaaa ggaaaagatg gagtagatgc atacactaaa
     3781 aaagtaaaag gtatgtgtaa aggggttaaa gtaaatgcaa tagatacaac cggagcagga
     3841 gattcttata taggttcatt tttatacacc ttattgtata aacaaataac attgaatgat
     3901 ataaaagaaa tgaaacaaga aacgttaaat gagtatttag aattttctaa ttattatgca
     3961 gctcgtagta caactattaa aggtgcgata agttcatatg caacaaagga agaaataact
     4021 gaatttataa aaaatttata gtaggaatga gtaataggtt ttgaaggaag taacatgtaa
     4081 agagttttta aggatgttta ctatgaaaat agcaataaag aggctgtctc aaaatagaga
     4141 taagcctctt tataattgtt gaaatgataa aaaatatttt aattattgtt actttttaga
     4201 agaatatata tcttatttta aataagcaaa ttatgtaaga tttatttata ttatcaatac
     4261 tttacttgta gtattatgtc tttagcagtt atctacttca attgaattat ctaaaatata
     4321 ataaatttgt tttgcaaatt gacgagaatg ttcttctttt tctttattca taaaattagg
     4381 tttacctaaa tcacgttcaa caagcattcc tatatatttt aactgtgaat gcttacagta
     4441 gcgcttcata ccattttccc aaatatcagc acccttttca ggtctatatc cacatgttgt
     4501 aataagtgct actggttttc ctgcccatag agcggggcct ttctcattac catagtattt
     4561 attcattcca tacaccaatc tgtcaagtag tgctttcatt ggagaagtgc agtaccaaga
     4621 ataaattgga gtagcaaaaa caattaaatc acacataaga attttcttga aaattttctg
     4681 aacatcatct ttacaataac atccaaaatc aaataagtta tcctgacatt ttttacaagc
     4741 aatacatggc tctattttac aatcatataa ccatattact tcaatatttg aattataatt
     4801 ttctaattcc tcaataaaag gttttaaaat agcagcagta ttaccttttt tcttaggact
     4861 tcccataaga atgcatactt ccatattgat acctccacag tattttaaaa tagtttttag
     4921 tgcttaatat agttttagga tactatgaga atacgttaac aacaatacac tttgtatatt
     4981 tgtaataaaa ttgaaacaaa ttgttttagc aataaaagtt ggaatgtatc tataattaaa
     5041 aatattttag gtatgttttt acatatatgt gtgtagttaa ctatggtaat ttattgtaaa
     5101 attatcttat aggattataa ttgcttaatt tatgattata tttaaaatct tggaggtaaa
     5161 aaatatgtac tcaaatgaag actataaatt tatagaagaa aatgcaccat gttatatata
     5221 tgatgagact aatataattg aaaaatgtaa ggaattgaaa aataatattt taggttttga
     5281 ttttttatat tctataaaaa caaatccata taacaaaatt gtaaaaagca ttgcagagca
     5341 aggttttgga gcagatgctt cttcaactaa tgaagttatg atttcatcaa aatgtggagt
     5401 ttctaaagat gatatttatt attcatcacc aggcaaaaca gagattgata tagaaactgt
     5461 tttagataaa tgcataatta ttgcagatag tattacagaa atagaactta taaataagat
     5521 atctaaaaag cataatcaga aagttaaaat aggaataaga ataaatcctg attttactat
     5581 ggatgatgat agtgctaaat caagcaaatt tggtatagat atagaagata tgagcagtgt
     5641 ttttaagatg ttaagtatgt gtgattatgt atctgtagtt ggtattcata tacatattaa
     5701 atctcaggta ttggattttg aaaagttagg acattattat gaaaaatgtt tttttatagc
     5761 tttaaattta aataaaagta agtatattaa tattgaattt ataaattttg gtagtggact
     5821 tggagtagct tatgataatc ttagagaaaa tccacttaat cttaaagagc tttcaaaatt
     5881 aactcaaaag attgctttta ctaattcaaa cacactaaaa tcaagacttc ttattgaatc
     5941 aggacgtttc atagtttgta aatcaggtaa ttattatgtt aaaattgtag atattaaagt
     6001 atcaagaggg aaaaaatatc ttattgtaaa aaatggactt aatggattta tgagacctat
     6061 tatttctaat ttaattgaaa atattacttg tggagtagaa ttagaaggtc aagagccatt
     6121 atatacatca aatcatgcct tttctctttc agtattaaat aatttaaaag aagaagagta
     6181 tgtaaatgtt gttgggaatc tctgtacatc attagatgta attgcaaata atgttaaatt
     6241 gaaaaaagca aacataggag atttgatttc aattagtaat gctggaagtt actcctattc
     6301 tttatcaccg ctattatttt caagttatga atttcctaaa caatattata aaagaaaaga
     6361 tggatatatt gagtaaacaa tagcataaat taataaactt agtattattg aaaaaattta
     6421 aaatgcaggg ggtgtaattc tatatggaat tgtggaaaag aaatttattt gtatgttgga
     6481 taggtatgtt ttttagttct atagggatga gccaaatagc acctatactt ccattatata
     6541 ttaaacagtt gggggtaact gatgttagtt taatacaaca gtattcaggt ataatttttg
     6601 gatgtacctt tgtagtagca gcattctttt cgccaatttg agggaaagct gctgataaat
     6661 atggaagaaa atcaatgctt ttacgtgcaa gtattgggat gggaatagtt atatttacca
     6721 tggcgtttgt tcaaaatgtc tatcagttat taggattaag aatacttcaa ggagttttta
     6781 cgggttatgc tactgcatgt actactctta ttgcaacaca gacagataaa aatcattcag
     6841 gatgggcttt gggtactctt gcaactgcat caactactgg ctcattaatt ggtccaacag
     6901 taggaggata tatagaatca atcttaggtc ttaaaagtac atttattata acaggaggat
     6961 tgttgtttgt atcatttatc atctctattt tatttgtagt agataatttt aaacctaaag
     7021 aaataaaaga atcaatttct atacctaaag aacaattaaa tatattgcca aataaatttt
     7081 taattgcatc tatttttgta accacattca ttactcaact agctttatac tctatagaac
     7141 caattgtaac catatatata tcacaactta caaattttgc atcaaatgta gctttaatag
     7201 ctggattgac attttctgca tcaggattag caaatttatt agcagctcaa aaacttggaa
     7261 aaatgtcgga taaaataggt cctcaaaaag tattgttaat ttcactttta tgggctggga
     7321 ttatatttat accacaagct tttgtaaaaa ctgcatggca actgatgttg ttgagatttt
     7381 tattagggtt gtcagttgca gggcttaatc cttctgttaa cagcttactt aaaaaaattg
     7441 caccagaaga atatgtggga aaaatatttg gatataatgc atctgcccag tatataggct
     7501 gttcatcagg agcattttta gggggacaaa tatcagcaca tttaggtatt agaacagtat
     7561 ttttttcaac aagtttattg ttgtttatga atgcactttg gatgtataaa tttactggat
     7621 tgtttattaa agataagact aaataatcat actatttaca tttctaattt tatgatgtaa
     7681 aataaatatt ttttatgtaa tttatcatag aattatataa aaaataaccc tatggtaaaa
     7741 attggaaaat atttttatcg acaaaatctt tgacttaaaa actatattgc gatagtatta
     7801 atttaataaa agtttgagag gggcgtaagg ttatgacaat ttcaaaaata ccacaagcag
     7861 aattaaaagt tatgaagttt atttgggaaa aaaatgatac agtaacatca aaagaaacta
     7921 ttgaagctat ggaacgaaaa tatggttgga aacaaacaac tacattgaca cttttatcaa
     7981 gacttgttaa aaaacatttt ttaagtgcag aaaaaataga tagatacact cattatacaa
     8041 tagttgttgg acataaggag tatttaagtg ttgagacaaa agatttttta agcaatatac
     8101 acgacaattc tttacagagt ttaatttcag cactacatga tgatgaagtt gttgataaag
     8161 atatgttgga tttatttgaa agtcatttta aaaatttaga agaagagtaa ttatatcagg
     8221 atagaactct aagaatttaa ttaatctatg gctgaaaacc ataaatggta ttatttaatc
     8281 agctactcac tattcataaa gtaatattaa ataggttctc taattagtaa aatgcctaaa
     8341 ataattggaa tagggagtat caagttaaat gataccactt attta
//
LOCUS       DBGJFF010000150         8342 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.153, whole genome shotgun sequence.
ACCESSION   DBGJFF010000150 DBGJFF010000000
VERSION     DBGJFF010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8342
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.153"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            108..263
                     /locus_tag="ACOIAF_003348"
     CDS             108..263
                     /locus_tag="ACOIAF_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670341.1"
                     /translation="MKGILKELTAKLYSFFYFMFWGFYFSAGYFFYKNICTSFLVRPY
                     FSDFEHK"
     gene            393..1406
                     /gene="aroF"
                     /locus_tag="ACOIAF_003349"
     CDS             393..1406
                     /gene="aroF"
                     /locus_tag="ACOIAF_003349"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HGZ2670342.1"
                     /translation="MIVVLKMGADKNEVKKLIEAIGREGVEVNPIDGTELTVLGLVGD
                     TSKIDAKRIEANKVVEKVMHVVEPFKKANRKFHPEPSIINVNGMEIGSKKIAMIAGPC
                     SVETEDQIVSIAKDVKKSGAGFLRGGAFKPRTSPYAFQGLKYDGLDLLKKAKEKTGLP
                     IVTEIMSTQDIDIFEENVDVIQVGARNMQNFDLLKELGKTNKTILLKRGLSATIEEWL
                     MSAEYIMAGGNENVVLCERGIRTFETYTRNTLDLSAILAVKKLSHLPVIVDPSHAAGK
                     SWMVDSLSKAAIAVGADGLIIEVHNDPAHALCDGKQSIKPNEYDELISELKTIASAVG
                     REI"
     gene            1428..2492
                     /gene="aroB"
                     /locus_tag="ACOIAF_003350"
     CDS             1428..2492
                     /gene="aroB"
                     /locus_tag="ACOIAF_003350"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HGZ2670343.1"
                     /translation="MEEKLLVNLKENSYNIFIKKNILKNIGEEIKKIYFGNKIFIVTD
                     ENIAKLYLDKVKNNLSESGFEIKSLILKSGEETKSFNTLPKIYDSLLDFKLTRKDLII
                     TLGGGVIGDLGGFAAATFLRGISFIQIPTSLLAQVDSSVGGKVGVDLERGKNLVGSFY
                     QPKAVFIDPNVLLSLPEKFYRDGMAEVIKYGCIKDKTFFNKLLSLKNREEVMQHIEYI
                     IHKCCFIKKTVVEADEKDFGDRMLLNFGHTLGHAIEKYYNFTGYTHGEAVAIGMYKIT
                     LESEKIGITKKGTSELIKSILMNYNLPYEIELRDNKSIVDAISLDKKNLGDTLKIILL
                     KDIGESIIYNTTSKFFQEVR"
     gene            2492..3805
                     /gene="aroA"
                     /locus_tag="ACOIAF_003351"
     CDS             2492..3805
                     /gene="aroA"
                     /locus_tag="ACOIAF_003351"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HGZ2670344.1"
                     /translation="MGSLKIYPSKLSGDVKIPPSKSMAHRAVICSSLSNGKSRISNID
                     FSDDIIATIRAMTSLGAIIEKKEDILEISGIFSKEGILNRENQLNQPKLTIDCNESGS
                     TLRFLVPISLTFDGVKRFIGRGNLGKRPLDTYYEIFDRQNIKYSYKENQLDLIISGKL
                     KPDEFRVKGNISSQFITGLLFILPTLESDSKIIITTELESKGYLDLTLSTIKDFGVEI
                     INNNYKEFIIKGNQTYKARDYKVEGDYSQGAFYLSADAIGEDISILDLKEDSLQGDSE
                     VVEILSRMGMEILREGNKIKGITNGLNGTLIDASQCPDIIPVLSVVASLSIGKTTIIN
                     AGRLRIKECDRLHAINVELSKLGANIEEKEDSLIIEGVSKLNGGVEVWSHKDHRIAMT
                     LAIASCRCDKPIILKDFECVSKSYPHFFKDFKMLGGRIDEWNMGK"
     gene            3783..4862
                     /gene="aroC"
                     /locus_tag="ACOIAF_003352"
     CDS             3783..4862
                     /gene="aroC"
                     /locus_tag="ACOIAF_003352"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HGZ2670345.1"
                     /translation="MSGIWGNNLKVSIFGESHGNAIGINIDGLPSGIELDLDNIDKEM
                     KRRAPGKNSISTSRNESDIPEILSGYFNGRTTGTPLCAIIRNSDTRSKDYGELKNLMR
                     PGHADFTGNVRYSGFNDYRGGGHFSGRITAPLVFCGAICKQILSQKGIEIGAHIKKIK
                     NIEDMSFDYVNISKQQLSNLQTLELPLLDLSKEEAMKNTIIDAKNQGDSVGGIIECAV
                     VGINVGLGNPFFDSVESTLSHLLFSVPAVKGVEFGLGFELADMYGSQSNDEMYYEGNQ
                     VKSKTNNNGGIIGGITTGMPIIFKVAIKPTPSISRQQNTVNIKDKKDDILYIKGRHDP
                     CIVQRAIPVIEAVTAIGIFDLMKGR"
     gene            4865..6061
                     /gene="pheA"
                     /locus_tag="ACOIAF_003353"
     CDS             4865..6061
                     /gene="pheA"
                     /locus_tag="ACOIAF_003353"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HGZ2670346.1"
                     /translation="MEDLTRYRNEIDNIDKELIQLFEKRMNTVLEIARHKMKNNTTIL
                     QKDREEKVLSRAVDNLDNKAYSQETVQFFNSIMEISRGLQKRLIDNDGEQKIEFGLDS
                     SNKNFDLSNVNKYKSLKDELNKKNILVGFPGKSGSFTEEALNKFFNKKISKKQFKEFE
                     DVFIALKNKEINYGIIPIENSSTGAISETYDLLRKYGFYIVGEECIKIDQNLIGIKDT
                     KLEDIKEIYSHPQGIGQCSEFLKQNSTWKLIPFNNTATSAELVRKLQDKTKAAIASKK
                     AASIYGLEIISPCINDIANNYTRFVVISNQIHIEEESNKMSVVFSVEHEAGKLYKVLG
                     YFAKNNINMTKIESRPMKNASWRYFFYIDFECSIYNAKVYDLLELIEHNTAYFKFMGV
                     YKNKTE"
     gene            6108..6917
                     /gene="aroE"
                     /locus_tag="ACOIAF_003354"
     CDS             6108..6917
                     /gene="aroE"
                     /locus_tag="ACOIAF_003354"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HGZ2670347.1"
                     /translation="MNFFGLVGEKLSHSVSPQIHKRVFEILNIESAYKNFEISKEDIS
                     KLDGAIKLLGIQGVNVTVPYKERIMKYLDFISPEAKRIGAVNTILLRENMLYGYNTDY
                     FGLDSMFKMANIDVQGKVAVILGTGGASKAALTYFIDSGIEKLYVSTRKKDDKKLLNS
                     KAILIDYEELKHIKGDIILNATPVGMYPNVGISPVSKSIIQNFDILIDLIYNPGETEF
                     LRIGNSMGKKTCDGLYMLVGQAIKSQEIWQDTKIDNSILDVIYNELKLEFL"
     gene            6929..7462
                     /locus_tag="ACOIAF_003355"
     CDS             6929..7462
                     /locus_tag="ACOIAF_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HGZ2670348.1"
                     /translation="MINKTKEKLILIGMPGSGKTTIGKLLAKEYNCSFCDMDDYIIQI
                     SQKSIAELFSEGEDIFRNYETQACRELSISDKTVISTGGGVIKKDVNMEILKETGIII
                     FIDRPIQKILEDININSRPLLKNGKDRLYNLYNERINLYKKFSDIEILNDKSLNNAVY
                     NITNAVSENFKFDFKEK"
     gene            7483..8322
                     /locus_tag="ACOIAF_003356"
     CDS             7483..8322
                     /locus_tag="ACOIAF_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HGZ2670349.1"
                     /translation="MNIVIVGLGVIGGSFAKALKKAGYENVFGVDVDLETLKKAEKAK
                     IIKKGCTTGKELFKKADLIILSIYPRLVVDFLNNNKNFFKKGTIITDTTGIKETLIND
                     VLQIIPDDIDFIFGHPMAGREKKGIDFASEQVFNGANYIITPTGRNNIKNLELVENLI
                     LEIGFKRVKKLTSQKHDEIIAFTSQLPHVMAVALINSDEEGRDTGKFIGDSYRDLTRI
                     ANMNEDLWSELFLGNRDNLLKVIENFESEVNLVKEAIFNNDKNKLIEYFKKSSIRREA
                     LEK"
BASE COUNT         3430 a          831 c         1502 g         2579 t
ORIGIN      
        1 aatattttaa attgaaaata tttatagaaa atattgacaa ttatattttc tttgttataa
       61 ttattaaaca agaagaataa taaattataa aaatggagga taatactatg aaaggaatat
      121 taaaagaact aacagcaaaa ctttatagct ttttttactt tatgttctgg ggcttttatt
      181 ttagcgctgg ctatttcttt tacaaaaata tatgtacttc ctttctggtg aggccttatt
      241 ttagtgattt tgagcataag taaattttat aaaaatgatt attttatata tttatataag
      301 atttagtgct tgatagatta ttttagagaa ctcattagtg agttctcttt tttattttaa
      361 atataaaatt ataatattgg gaggtagtaa aaatgatagt agtattaaaa atgggagcag
      421 ataaaaatga agttaaaaaa ttaattgagg ctattgggag agaaggagta gaagttaatc
      481 ccatagatgg aacagaatta actgttttag gtttagttgg agatactagt aaaatagatg
      541 ccaaaagaat agaagcaaat aaggtagttg aaaaagttat gcatgtggta gagcctttta
      601 aaaaggccaa tagaaaattt catccagaac cttcaatcat aaatgttaat ggaatggaaa
      661 ttggttcaaa gaaaattgct atgatagctg gaccttgttc tgttgaaaca gaagaccaaa
      721 tagtatctat agctaaagat gtgaaaaaat caggtgcagg atttttgaga ggtggagcat
      781 tcaaaccaag aacatcacca tacgctttcc aaggattgaa atatgatgga ttggatttat
      841 taaaaaaagc aaaagaaaaa acagggcttc ctatagttac agaaataatg tcaacacaag
      901 atatagatat atttgaagaa aatgtagatg taattcaagt tggagctaga aacatgcaaa
      961 actttgattt attaaaagaa ttaggtaaaa caaataaaac aatactttta aaaagaggat
     1021 tatctgctac aattgaagag tggctaatgt cagcagagta tataatggct ggaggaaatg
     1081 aaaatgtagt tctttgtgaa agaggtataa gaacttttga aacatatact agaaatactt
     1141 tggatctaag tgcaatttta gctgtgaaga aacttagtca tttaccagtt atagttgacc
     1201 caagtcatgc agcaggtaaa tcatggatgg ttgactcttt atctaaggca gcaattgcag
     1261 ttggagcaga tggtctaata atagaagttc ataatgaccc agcacatgca ctttgtgatg
     1321 gaaaacaatc gattaagcca aatgaatatg atgaattgat aagtgaatta aagactatcg
     1381 catcagcagt tggtagagaa atataatcta taaaaggatg gttaaacgtg gaagaaaaac
     1441 tattagtaaa tttaaaagaa aatagctaca atatatttat taaaaaaaat atactaaaga
     1501 atataggaga agaaataaaa aaaatatatt ttggaaataa gatttttatt gtaactgatg
     1561 aaaatatagc aaaattatac ttggataaag taaaaaataa tttaagtgaa tctggatttg
     1621 aaattaaatc tttaatatta aaatctggtg aagaaacaaa atcatttaat actcttccta
     1681 aaatatacga tagtttactt gactttaagt taactagaaa agatttgatt attactttag
     1741 gaggaggggt aataggtgat ttaggtggat ttgcagcagc aactttttta agaggtataa
     1801 gttttattca aattccaaca tctttattag cacaagttga tagttctgta ggtggaaaag
     1861 ttggagttga ccttgagaga ggcaaaaatt tagtgggaag cttttaccaa ccaaaagctg
     1921 tgtttatcga cccaaatgta ttattatcac ttccagaaaa attctataga gatggaatgg
     1981 cagaagttat aaaatatgga tgtataaaag acaaaacttt tttcaataaa cttttaagtt
     2041 taaaaaatag agaagaagta atgcaacata tagaatacat tatacataaa tgttgcttta
     2101 ttaaaaaaac tgtagttgaa gctgatgaaa aagactttgg agatagaatg cttctaaact
     2161 ttggccatac attaggtcat gctattgaga aatactacaa ttttacaggt tatacccatg
     2221 gagaagctgt tgccatagga atgtataaga taactttaga aagtgaaaag ataggaatta
     2281 caaaaaaagg gacatcagaa cttataaaat caattttaat gaattataat ttgccttatg
     2341 agattgaatt aagagataat aaatctatag tagatgctat atctcttgat aaaaaaaatc
     2401 taggtgatac attaaaaata atacttttaa aagatattgg agagagtatt atatataata
     2461 ctacttctaa attttttcag gaggtaagat aatgggaagt ctaaaaatat atccaagtaa
     2521 attatctgga gatgtaaaaa taccaccatc aaaaagtatg gcacataggg cagttatttg
     2581 ttcatctcta agcaatggaa aaagtagaat ttctaatata gatttttctg atgatataat
     2641 agctactata agagcaatga catcattagg agctattata gaaaagaaag aagatatttt
     2701 agaaatttcg ggaatttttt ctaaagaagg tattttaaat agagagaatc agttgaatca
     2761 acctaaattg acaatagatt gtaatgaatc aggctctaca ttgagatttt tagtacctat
     2821 atctttaaca tttgatggtg taaaaaggtt tataggtaga ggtaatcttg gaaaaagacc
     2881 acttgatacc tattatgaga tatttgacag acagaatata aaatatagtt acaaagaaaa
     2941 tcagttagat ttgattatat caggtaagct taaaccagat gaatttagag taaaaggaaa
     3001 tataagttct cagtttatca caggtttact gtttatattg cctacgctag aaagtgattc
     3061 aaagataata attactactg agctagaatc taaaggatat ttagatttaa ctctttcaac
     3121 gataaaagat tttggggtag aaattataaa caataactat aaagaattta ttataaaagg
     3181 aaatcaaacg tataaagcga gagattataa agtagaagga gattattctc aaggagcatt
     3241 ttatttatca gcagatgcta taggagaaga tattagcata ttagacctta aagaagattc
     3301 tcttcaaggg gatagtgaag tagttgaaat tttatctagg atgggaatgg aaatattaag
     3361 agaaggcaac aaaataaaag gaataacaaa tggtttaaat ggtactttga tagatgcatc
     3421 tcaatgccca gatataatac ctgtattatc tgtagtagca tccttaagta tagggaagac
     3481 tacaataatt aatgctggta ggcttagaat taaagaatgt gatagacttc atgcaataaa
     3541 tgttgagtta agtaaacttg gagcaaatat agaggagaaa gaggatagtt tgataatcga
     3601 aggagtaagc aagttaaatg gtggagtaga ggtgtggagt cacaaagacc atagaattgc
     3661 tatgacactt gcaattgctt cttgtagatg tgataaacca ataatattaa aagattttga
     3721 gtgtgtatca aaatcttatc cacatttttt taaagatttt aagatgttag gaggtcgaat
     3781 tgatgagtgg aatatgggga aataatttaa aagtatcaat ttttggagaa tctcatggaa
     3841 atgctatagg tataaatata gatggtcttc cttcggggat tgaacttgac cttgataata
     3901 ttgataaaga aatgaagaga agagcacctg gaaaaaattc tatatctact tcaagaaatg
     3961 aaagtgatat accagaaatt ctaagtggat attttaatgg aagaactaca ggaacgccat
     4021 tatgtgcaat cataagaaat agtgatactc gttcaaaaga ttatggagag cttaaaaatt
     4081 taatgagacc aggacatgca gatttcacag gtaatgtgag atattctgga tttaatgact
     4141 atagaggggg ggggcatttt tcaggtagga taacagctcc attagtattt tgtggggcta
     4201 tatgtaaaca aattttatca caaaaaggaa tagagatagg agcacacata aaaaaaatta
     4261 aaaatataga agatatgagt tttgattatg taaatatttc aaaacaacag ttatcaaatt
     4321 tgcaaacttt ggaactacct ttacttgatt tatcaaagga agaagccatg aaaaatacga
     4381 ttatagatgc aaaaaatcaa ggagattcag ttggtggtat aattgagtgt gcagtagtag
     4441 gtattaatgt agggcttggg aatccatttt ttgactctgt tgaatctact ttatcacatt
     4501 tattgttttc tgttcctgcc gtaaaaggtg tagagtttgg tttaggtttt gaacttgcag
     4561 atatgtatgg ctctcaaagt aatgatgaaa tgtattatga aggaaatcaa gttaagagca
     4621 aaacaaataa caatggagga attataggag ggattactac tggaatgcca ataattttca
     4681 aagtggcaat taagccaact ccatcaatta gcagacaaca aaacacagtt aatataaaag
     4741 ataaaaagga cgatatttta tatattaaag gcagacatga cccttgtata gttcaaaggg
     4801 ctatacctgt aatagaagca gtaacagcca ttggaatttt tgatttgatg aaaggacgat
     4861 aataatggag gatttaacga gatatagaaa tgagattgac aatatagata aagagcttat
     4921 tcaattattt gaaaagagaa tgaatacagt tcttgaaatt gccagacata aaatgaaaaa
     4981 taacactact atacttcaaa aagatagaga agaaaaagta ctaagtagag ctgtagataa
     5041 tttagataat aaagcatatt cacaagaaac ggtacagttt tttaattcta taatggaaat
     5101 tagtagagga ttacaaaaaa gattgataga taatgatggt gagcagaaaa tcgagtttgg
     5161 tttagattca tcaaataaga attttgattt gtcaaatgta aataaatata agtctttaaa
     5221 agatgaatta aataaaaaaa atatcctagt aggatttcca ggaaaatcag gttcatttac
     5281 agaagaagca ctaaataaat tcttcaacaa aaaaatatca aaaaaacaat ttaaagaatt
     5341 tgaagatgtt ttcatagcac ttaaaaataa agaaataaac tatggaataa tccccataga
     5401 aaattcatct acaggagcta tctctgaaac ttatgacctt ttgagaaagt acggttttta
     5461 tatagttgga gaagaatgta taaaaataga ccaaaattta atagggataa aagacacaaa
     5521 gcttgaagat ataaaagaaa tatattctca tccacaaggt ataggacaat gtagtgaatt
     5581 tttaaaacaa aatagtacat ggaaattgat accatttaat aacacagcaa caagtgcaga
     5641 acttgtaaga aaacttcaag ataaaacaaa agcagcaatt gcaagcaaaa aagctgcaag
     5701 tatttatgga ttagaaataa taagtccttg tataaatgat atagcaaaca actatacaag
     5761 atttgtagta atcagtaatc aaatacacat tgaagaagaa tcaaacaaaa tgagtgtagt
     5821 attttctgtt gaacatgaag caggaaaact ttataaagta cttggatact ttgctaaaaa
     5881 taatataaat atgacaaaaa tagaatcaag acctatgaaa aatgcctcat ggagatattt
     5941 cttttatata gattttgagt gttctatata caatgctaaa gtgtacgatt tattagaatt
     6001 gatagaacat aatacagcat attttaaatt tatgggagta tataaaaata aaactgaata
     6061 gataagataa atttttattt aatttataat tattaggagg tgtaagtatg aattttttcg
     6121 gtctagtagg tgaaaaactc tctcacagtg tttcacctca aatccacaag agagtattcg
     6181 agatattgaa tattgaaagt gcttataaaa actttgaaat ttcaaaagag gatatatcta
     6241 agctagacgg tgctataaaa ttattaggaa tacaaggagt aaatgttact gtaccatata
     6301 aggagcgtat tatgaaatat cttgatttta tatcaccaga ggcaaaacgt ataggagcag
     6361 ttaatacaat tttacttaga gaaaatatgt tatatggtta caatacagat tactttggtc
     6421 ttgatagtat gtttaaaatg gctaatattg atgttcaagg aaaagtagca gttatattag
     6481 gaactggagg tgcttctaaa gctgctctta catattttat tgattctggt atagaaaagc
     6541 tttatgtttc aactagaaaa aaggatgata aaaaattatt aaatagtaaa gctatattaa
     6601 tagattatga agaacttaaa catataaaag gagatataat attaaatgct acaccagtag
     6661 gtatgtaccc taatgtagga atttcaccag tgtcaaaatc tattatacaa aattttgata
     6721 tattaataga tttaatttac aatcctggag aaacagagtt tttaagaatt ggtaatagta
     6781 tgggtaaaaa aacttgtgat ggattatata tgctggtagg gcaagcaata aaatctcagg
     6841 aaatttggca agatactaaa atagataata gtattttaga tgttatatat aatgaactaa
     6901 aattagaatt tttgtaaggg agattgttat gataaataaa acaaaagaaa aattgatatt
     6961 aataggtatg ccaggtagtg gaaaaactac aattggtaaa cttttggcca aagaatataa
     7021 ttgtagtttc tgtgatatgg atgattatat aattcaaata tctcaaaaaa gtatagcaga
     7081 gttgttttca gaaggagaag atatctttag gaactatgag actcaagcat gtagagaact
     7141 ttctataagt gataaaacag tgatatctac aggtggaggt gtaataaaaa aggatgttaa
     7201 tatggaaata ctaaaagaaa caggtataat aatttttatt gatagaccta tacaaaagat
     7261 tttggaagat ataaatatta attcaagacc attattaaaa aacggaaaag atagattgta
     7321 taatctttac aatgagagaa taaatcttta taaaaaattt tcagatatag agattttaaa
     7381 tgataaatca ttaaataatg ctgtatataa tattacaaat gcagtcagtg aaaattttaa
     7441 atttgacttt aaagaaaaat aatctagggg gaaataaaaa taatgaatat tgttattgta
     7501 ggactaggag ttataggagg ctcttttgct aaagcattaa aaaaagctgg atatgaaaat
     7561 gtatttggag tagatgtaga tttagaaact ttaaaaaaag cagaaaaagc aaaaatcata
     7621 aaaaaaggat gtactacagg taaagagtta tttaaaaaag ctgatttaat tatattatct
     7681 atatatccta gattagttgt agattttcta aataataata aaaacttttt taaaaaaggg
     7741 actataatta ctgatactac aggaataaaa gaaacactta tcaatgatgt gttgcaaatt
     7801 attccagatg atatagattt tatatttgga catccaatgg caggtaggga aaaaaagggt
     7861 attgattttg caagtgaaca agtttttaat ggcgcaaatt atataattac tccaactggc
     7921 agaaacaaca taaaaaattt agagttagtt gaaaatttaa ttttagagat tggatttaaa
     7981 agagtaaaaa agttaacttc acaaaaacat gatgagatta tagcttttac ttctcagtta
     8041 cctcatgtta tggcagttgc tcttataaat agtgatgaag aaggtagaga tacgggtaaa
     8101 tttattggag atagttatag agatttaact agaattgcaa atatgaatga agacttatgg
     8161 agtgaacttt ttttaggaaa tagagataat ttactaaaag ttatagaaaa ctttgaatca
     8221 gaagtaaatt tagtaaaaga agctatattt aacaatgata aaaataaatt aatagagtat
     8281 tttaaaaaat catcaataag aagagaagct ttagaaaaat aaatattaat atttaagcta
     8341 ta
//
LOCUS       DBGJFF010000151         8316 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.154, whole genome shotgun sequence.
ACCESSION   DBGJFF010000151 DBGJFF010000000
VERSION     DBGJFF010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8316
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.154"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(<1..1023)
                     /locus_tag="ACOIAF_003357"
     CDS             complement(<1..1023)
                     /locus_tag="ACOIAF_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HGZ2670350.1"
                     /translation="MNLNLTNTINLMKKYLDIPSPAGYTENAVLEIKKDFENLGLDTI
                     LTKKGALIATLPGEDDSNQITISAHMDTLGAIVKEISSDGTLKYHKVGGGCWAAIEGE
                     NCTVITRKGKKIRGSVVFKYASTHIYGQTKAATERNEETMIIRLDEKVFTKQDVLDLG
                     ISVGDFVCLDHRFEATESGFIKSRYIDNKSAVAMVLEICRYFKENNLTPKHTTNFYIS
                     NYEEIGHGVSKCLPEKTKEFVAIDIAPTGPSQTSSEHGVTIVAKDNLSLYDFNLRNKL
                     VDVAEDNNLDYAVDVFTFYGSDASEAIRWGEDVQIACVGPGTNNSHHYERTHIEAIEN
                     TLKLLIN"
     gene            1493..2653
                     /locus_tag="ACOIAF_003358"
     CDS             1493..2653
                     /locus_tag="ACOIAF_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HGZ2670351.1"
                     /translation="MIETGFVYCSFLVVFVGVIFFMVDKLKWKVFDVINPMLLIYLGS
                     AILGSFGLFAQNEDVGTYQNIITVNFLPLMLVFLLIQCDLRKILKMGPKMLLSFFCAT
                     FTFMLGFVIAFIIFKNSMGADSWKILGAASGSWIGGSSNMIAAANALGVAEEGLSYAI
                     LMGSIGYTVWMSVCLMSVKFGPKFNKWTKCDTSFIDEVTSKLEKENKDNTAPTFVELM
                     ILLSVGFGVASFAQIVSKILPTSGILNETMWVVLVASTVSILLGMTKISKLKGSTLVG
                     NVFMYLLLAATGSKANFFGLSEAPVYIMFGLVVVLVHAISFIGLAKLFKLDLFTCEVG
                     SIANIGGVTTAPMIAGVHNPVLIPVGVLMGLLGNIIGTYCALMVTQILHMLA"
     gene            2995..3156
                     /locus_tag="ACOIAF_003359"
     CDS             2995..3156
                     /locus_tag="ACOIAF_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670352.1"
                     /translation="MIIPSRRIQDITLTNLKNGEVTLIELDEIYKKMGFLFVVSEGKL
                     KKIKKENHH"
     gene            3349..3978
                     /locus_tag="ACOIAF_003360"
     CDS             3349..3978
                     /locus_tag="ACOIAF_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HGZ2670353.1"
                     /translation="MKIVTYNIHKCMDSNNRLTLTKMGLYLKQLDCDVICLQEVLYPQ
                     FLALKAVLNMDGVFATNVKKVNMIYGICTFTTSKMLNSNHFFLTSKKEQRGALCITID
                     AYGRIINVINTHLGLDRQERAKQLDEIIDYRNRLVGIVILCGDFNEKNVFLSMFNDMA
                     ISLNKSYLPTFEKSNSRIDYIFVNKNTELKGYTVEKIYLSDHYPVIGYI"
     gene            4217..5476
                     /locus_tag="ACOIAF_003361"
     CDS             4217..5476
                     /locus_tag="ACOIAF_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HGZ2670354.1"
                     /translation="MGRRTTRVSRLGKEKKRSVLIVLAALVLIMGGFTYFFNSKFLYN
                     GKIAKNVYIEGVNVSDMTKAEALKAITDKYTPEDLNLAYDGKKYTISPKDIDLKYDTE
                     DVVKDAYESTKKGSYFQNLKKYIDIRVNKANMKIKAEYDEAKLSSKVSSIADSINVKM
                     KNASISVGSGGLSYTDSVVGREFDLAANKESIYNMIKNKEHKTLELKVNLQKPDITTE
                     QVKTVNSVIGQYSTTYSQAVEGRSYNVGLSARKTSDVLLMPGEEFSYNKLTGPSNKAN
                     GYKDAPVIVYGKLEQSAGGGVCQTSSTVYNAALLSGMEITQVTNHSSASTYVPKGRDA
                     TVSDGGLNLKFKNPYKHPVYIKNYAGGGSVSSVIYGNSGDKPNISIEVKQTGQNKYST
                     YRIFKDSSGKVIKKEHISNSSYKELKK"
     gene            5747..6730
                     /locus_tag="ACOIAF_003362"
     CDS             5747..6730
                     /locus_tag="ACOIAF_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HGZ2670355.1"
                     /translation="MTWLNFIGNRYEVVNSDDVLEINKIYKARDVFYRKNVLIKVIKH
                     NNYICEDFVSNLIDESTALDEINSPYILKIIDVGIHCTEETTLYYVVSEDFNGIGLDE
                     LILGNYLHLEAIVNIMIQVLKALEMIHRNHSYHGSLKSSSIIVDTEYNIKICDFGITK
                     ANNGVNTRSYGNRRYLCPHQLCINYTDKESDFFATGLILFESIFKKLPFGESNSEEKM
                     LQSIDKGLDWNSIKAINGNTELINVIKRLLGRNNKYTRANDIIIDLSKVMYEKAYIEV
                     EDEKEEEQKQDKVIQVKKEYKSKMRKKLHKKLVVAGLAIVMISMIIISSII"
     gene            6976..8043
                     /locus_tag="ACOIAF_003363"
     CDS             6976..8043
                     /locus_tag="ACOIAF_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HGZ2670356.1"
                     /translation="MSNIKIIASNKCWIEDIARKQLEDISKLNGILRVVGLPDLHAGK
                     IPVGLAVETKNIIYPHIIGNDIGCGMTLFKTGVLKKKFKKDKWIKSLSKIKDLSDIEI
                     KNTYKEECPILNLGTIGSGNHFVEIQCISEIYNKEQFEQLKFSSDDIMMLVHCGSRNY
                     GEDILKKFYDKDGLEVESEKAIDYIKKHDNALMWAERNREAISKKIMQSIGTSGDVET
                     IFSINHNFIEKREDKFIHRKGAVSSERGAVIIPGSRGSLSYIVMPTENTEISLYSLSH
                     GAGRKWSRSVCKSRLKGKYSKDTIKQTKFKSQIICNDVNLLFQEAPEAYKNIEQIIES
                     LIEYELIQVVATMKPLITYKG"
BASE COUNT         3241 a          885 c         1344 g         2846 t
ORIGIN      
        1 atttataagt aattttagtg tattttcaat agcttctata tgtgttcttt cataatggtg
       61 actattgttt gttccaggac caacacatgc tatttgaaca tcttctcccc aacgaatagc
      121 ttctgaagca tctgaaccat agaaagtaaa tacatcgaca gcgtagtcta aattattgtc
      181 ttctgcaaca tcgacaagct tgtttcttaa attaaaatca tataatgata aattatcttt
      241 tgcaactata gttacaccat gttcactaga tgtttgactt ggtccagtag gtgctatatc
      301 tattgctaca aattcttttg ttttttcagg taaacattta gaaacaccat gtccaatttc
      361 ttcataatta cttatataaa agttagtagt atgcttaggt gttaaattgt tttctttaaa
      421 atatctacat atttcaagaa ccattgctac agctgattta ttatctatat atctagattt
      481 tataaaacca gattctgtag cctcaaatct atggtctagg catacaaaat ccccaacact
      541 tattcctaaa tctaatacat cttgctttgt aaatactttt tcatcaagtc taataatcat
      601 tgtttcttca tttctttcag tggcagcttt tgtttgacca tatatatgag tagaagcata
      661 cttaaataca acacttcctc tgattttttt accttttcta gttataacag tacagttttc
      721 tccctctata gctgcccaac atccaccacc aactttatgg tatttaagag taccatcaga
      781 tgatatttct tttactatag ctccaagtgt atccatatgt gcagatattg ttatttgatt
      841 tgaatcatct tcaccaggaa gagtagctat aagtgcacct ttttttgtaa gaatagtgtc
      901 aagacctaaa ttttcaaaat cttttttgat ttctaaaact gcattttcag tatatccagc
      961 tgggcttgga atgtccaaat attttttcat taaatttata gtattagtta aatttaaatt
     1021 catattgttt cctcctaaat aaatatattt atacaaatta taacataaat acaaatgttc
     1081 tgaaaactaa aattaaaaga atagaatcta aacatattta aaaaaaaagt atataaaata
     1141 aatttgattt atattatata gatttaaagt aaaaatagtt ttatttaata attaataaaa
     1201 ataaatataa aacaaaataa ataaataata aaatatttag tttaattatc tttacaaata
     1261 aaaattttag taataaaata attgttgaaa aagattttaa attcagatta ttcatttcta
     1321 aagtaattaa tgtgcaaatc tttattaata tatgtcaata gttaggtata tatgtattta
     1381 attagtatga tatagataaa agtatttatt ttaattgtct attatctgaa aaagtcttta
     1441 cttagaaatc ataaaaatca aattaattag gaaaagaaag ggtgtgtata atgtgattga
     1501 gactggattt gtatattgta gtttcttggt tgtattcgta ggtgtaattt tttttatggt
     1561 tgataaattg aaatggaagg tatttgatgt tattaatcca atgcttttaa tatacttagg
     1621 ttcagctata cttggatcat ttggtttatt tgcacagaat gaagatgttg gaacgtatca
     1681 aaacataata acagtgaact tccttccatt aatgttagtg tttttgttaa ttcaatgtga
     1741 tttaagaaaa atattaaaga tggggcctaa aatgttatta tcatttttct gtgctacatt
     1801 tacatttatg cttggatttg ttatagcatt cataattttt aaaaattcaa tgggtgcaga
     1861 ttcatggaaa attcttgggg cagcatctgg ttcatggata ggtggctcat caaatatgat
     1921 tgcagcagct aatgctctag gagttgcaga agaaggactt agttatgcaa tacttatggg
     1981 ttctatagga tatacagtat ggatgtctgt ttgtctaatg tctgtgaaat ttggacctaa
     2041 atttaacaaa tggacaaagt gcgatacaag ttttatagat gaggttacat caaaacttga
     2101 aaaagaaaat aaagataata cggcacctac gtttgtagaa cttatgattt tactttctgt
     2161 tgggtttgga gttgcgtctt ttgcacaaat tgtatccaag atacttccaa cttcaggtat
     2221 acttaatgaa actatgtggg tagtacttgt tgcatctaca gtatcaatat tattgggtat
     2281 gacaaaaata tcaaagctta aagggagtac actggtagga aatgtgttta tgtatttact
     2341 ccttgcagct acaggtagta aagcaaattt ctttggattg tcagaagcac ctgtttacat
     2401 aatgtttgga ttggtagtag tccttgtaca tgctatttca tttatagggc ttgctaaact
     2461 atttaaactt gatttattta cttgtgaagt aggaagtata gctaatatag gaggagtaac
     2521 tacagctcca atgattgctg gggttcacaa tcctgtatta ataccagtag gtgtacttat
     2581 ggggcttcta ggaaatatta tagggactta ttgtgctctt atggtaactc aaatattaca
     2641 tatgttagct taataaaaat atggtataaa attaataatt taaataagaa aagtcatttc
     2701 aatttaagca gaagctttta ctgaaatggc ttttttgttt gacaagataa tctatttcaa
     2761 gaagatgttt tgacatattt agtgtataat attgtaaaat ttactaaaaa cgagatatat
     2821 ttaattttta tattgaatcg aacgtctgta tgatgtataa taaatataca gaacgaatgt
     2881 ttgcagaacg ggtgtttttt gaatttattt taataattta tgaatatata tcagaatgca
     2941 taaatatatt taaatataaa gattaaaaag aatttaaagg agtgttgata acttatgata
     3001 attccatcaa gaagaataca agatataaca ttaacaaatt taaaaaatgg tgaagtaact
     3061 ttaattgaat tggatgaaat atacaagaag atgggatttt tatttgttgt tagtgaaggc
     3121 aaattgaaaa agataaaaaa ggagaatcat cattagaaat gaatgaaata ataaatatta
     3181 taatagttta taaaacctaa cttatgtaga atataaatac ttaagttagg tttttttgtg
     3241 ataaagttta taagtactca attttataag ataaataatg gtcttaagtg aaaagtaaat
     3301 aagtttaaat tttagtaagt attatattgc ataagaggtg atattattat gaaaatagta
     3361 acctacaata ttcataaatg tatggattca aataatagat taactctaac taaaatgggg
     3421 ttatatttaa aacaattgga ttgtgatgta atttgtttgc aagaagtatt atatcctcaa
     3481 tttttagctt taaaagctgt tttaaatatg gatggtgtct ttgcgacaaa tgtaaaaaaa
     3541 gtaaatatga tatatggtat ttgtacattt acaacatcta aaatgttaaa cagtaatcat
     3601 tttttcttaa ctagcaaaaa agaacaacgg ggagctcttt gtataactat agatgcatat
     3661 ggaagaatta taaatgtaat aaatactcac ttgggacttg ataggcagga gagagcaaaa
     3721 caattagacg aaataataga ctataggaac agattagtgg ggatagttat tttatgtggt
     3781 gattttaatg agaaaaatgt ctttttaagt atgtttaatg atatggctat ttcactaaac
     3841 aagtcttact taccaacatt tgaaaaatca aactctagaa tagattacat ttttgttaat
     3901 aaaaatactg aactaaaagg atacacagta gaaaaaattt acctgtcaga ccattatcct
     3961 gtaataggtt acatttaaat ttcaatataa atgaaattag ttgttataaa ataaacaatt
     4021 ttttgttaag aaatagtaaa agaaatataa taaaaaatat caaaaataaa actaattata
     4081 agacataaat ttacaataaa gttacatttg attaaattcc ttgtttaaca aaagctattc
     4141 aaatataatc taaggtagtt ttgaaatggg aactctatta tgcaaattta agctagtgtt
     4201 aaaatgaggt gaagttatgg gcagaagaac aacaagagtt tcaagattag gtaaagaaaa
     4261 aaaacgaagt gttttaattg tattggcagc tttagttcta ataatgggtg gatttactta
     4321 tttttttaac agtaaatttc tgtacaatgg gaaaatcgct aaaaatgtat atatagaagg
     4381 agtaaatgta tctgatatga ctaaagcaga ggcattaaaa gctataacag ataagtatac
     4441 tccagaagat ttaaatcttg cttatgatgg gaaaaaatat acaatatcac caaaagatat
     4501 tgatttaaaa tatgatacag aagatgttgt aaaagatgct tatgaaagta caaaaaaagg
     4561 gtcctatttt caaaacttaa aaaaatatat tgatataaga gtgaataagg caaatatgaa
     4621 gataaaagct gaatatgatg aggcaaaact tagttccaaa gtgtcttcta tagcagatag
     4681 tataaatgtt aagatgaaaa atgcttctat tagtgttgga agtggtggac taagttatac
     4741 agactctgtt gtaggtagag aatttgattt ggcagcaaat aaagagtcta tatataatat
     4801 gataaaaaat aaagagcata aaactttaga acttaaagta aatcttcaaa aaccagatat
     4861 aacaactgaa caagtaaaaa ctgtaaattc tgttataggt caatattcaa ctacttattc
     4921 acaagctgta gagggtaggt catataatgt aggattatca gcaagaaaaa ctagtgatgt
     4981 attattaatg ccaggtgaag aattttcata caataaactt acaggaccta gtaataaagc
     5041 aaatggttac aaagatgcac ctgttatagt ttatggtaag ctagaacaat cagcaggagg
     5101 aggtgtttgt caaacatctt caacagttta taatgcggct cttctttcgg gtatggagat
     5161 aactcaagtt acaaatcatt catctgcatc tacttatgtg cctaaaggga gagatgctac
     5221 tgtgagcgat ggaggattaa acttaaagtt taaaaatcca tataagcatc ctgtatatat
     5281 taaaaattat gcaggtggag gaagtgtatc atcagtaatc tatggtaatt ctggtgacaa
     5341 accaaatata tctatagagg taaaacaaac tggtcagaat aaatattcta catatcgaat
     5401 atttaaagat tcaagtggaa aggtcataaa aaaagaacat atatcaaata gttcttataa
     5461 agaattaaaa aaatagataa ttttttagag aaatatttgt aatataaaag ggagtgtctc
     5521 aaaatgaact ttttagttta tgaggcactc tttttctgtg aaatcaaata tattttcatc
     5581 atttcaacaa tacaaaagaa gcgtatctct attttgagac agcctttttt gtaaaaatta
     5641 acactattag ttcttgtttt aatattacaa tcattaatat atagaaaata ttcctatata
     5701 ttggtataat tgtaaataga tttagttata taaaatgtat gaggaggtga catggttgaa
     5761 ttttataggc aatagatatg aggtagtaaa ttctgacgac gttttagaaa ttaacaaaat
     5821 ctataaagct agagatgttt tttacaggaa aaatgtgtta ataaaagtta taaagcacaa
     5881 taattatatc tgtgaagatt ttgtttcaaa tttaattgat gaaagtactg ctttagatga
     5941 gataaactct ccttatatac taaaaataat agatgttggg attcactgta cagaagaaac
     6001 aacattatat tatgttgtaa gtgaagactt taatggtatc ggactagatg aattaattct
     6061 aggtaattat cttcatttag aagcaattgt aaacataatg atacaagttt taaaagcttt
     6121 agaaatgata cataggaatc attcatatca tggtagttta aaatcgagta gtataatagt
     6181 agatacagaa tacaatataa aaatttgtga ttttggaatt acaaaagcaa ataatggggt
     6241 taatacaaga agctatggaa acaggagata tttatgccca catcaactat gtataaatta
     6301 tactgataaa gaaagtgatt tttttgcaac aggtcttatt ctatttgaat ctatatttaa
     6361 aaaattgcct tttggagaat caaattcaga agaaaaaatg ctacaatcta ttgataaggg
     6421 attagattgg aatagtataa aagcaattaa tggaaatact gaacttataa atgttataaa
     6481 aaggcttctt ggaagaaata ataagtatac cagagccaat gatattatta ttgatttaag
     6541 taaagtaatg tatgaaaaag catatataga agttgaagat gagaaagaag aagagcagaa
     6601 acaagacaaa gttatacaag ttaaaaaaga atataaaagt aaaatgagaa aaaaattaca
     6661 taaaaaactt gttgttgccg gtctagctat agtcatgatt tcaatgatta ttattagttc
     6721 cataatataa aaattgtata ttaagtattt taaaatcaat gtctataaag gatatacata
     6781 gaaattttag tgtagtctta aattatgaaa ggaagtggtt attatgttaa caaaaaataa
     6841 tgaggtatta aacgtctagg agtattttaa caaatagcca cctgctttta cagtttagaa
     6901 taaaaaagct gtggctaaag gtcacagctt tttgctactt tttaaaggca ggagaatatt
     6961 aaaaaggagc aaattatgag caatataaaa ataatagcaa gtaataaatg ttggattgaa
     7021 gatatagcaa ggaaacaact tgaagatatt tcaaagttaa atggaatttt aagagtagta
     7081 ggattacctg acttacatgc aggtaaaata cctgtaggtt tagcagttga gactaaaaat
     7141 attatttatc cacatataat tggaaatgat ataggatgcg gaatgacttt atttaaaact
     7201 ggggttctaa aaaagaaatt taaaaaagac aaatggatta aatcacttag taagattaaa
     7261 gatttgtctg atatagaaat aaaaaacaca tataaagaag aatgtccaat tttaaattta
     7321 ggaactatag ggagtggaaa tcattttgta gaaattcaat gtataagtga aatttataat
     7381 aaagaacaat ttgaacaatt aaaattttca tctgatgata ttatgatgtt agttcactgt
     7441 gggtcaagaa attatggaga agatatttta aaaaaatttt atgataaaga tggacttgaa
     7501 gttgaaagtg agaaagctat agattatatt aaaaaacatg ataatgcttt aatgtgggca
     7561 gagcgtaatc gtgaagctat cagtaaaaaa attatgcagt caataggtac atctggagat
     7621 gttgaaacaa tattctctat aaatcataat tttatagaga aaagggaaga taaatttata
     7681 catagaaaag gtgctgtttc aagtgaaaga ggagctgtga ttataccagg ttcaagaggc
     7741 agtctatcat atatagttat gcctactgaa aatacagaga tatctttata ttctctatca
     7801 catggagcag gtaggaaatg gtctcgttca gtttgtaaat ctcgccttaa aggtaagtat
     7861 agtaaagata ctataaaaca aactaaattt aaaagtcaga ttatatgtaa tgatgtaaat
     7921 ttattatttc aagaagctcc agaggcatat aagaatatag agcagattat tgaaagtttg
     7981 atagaatatg aacttattca agtagtggca actatgaagc cattgattac ttataaagga
     8041 taatttttaa agttaaaaaa gattaaaatt atattataaa tagctaattt tatagaacat
     8101 ttaaaagtgt tttattatta gctatttttt tattttgtag agacgataat ttatagagct
     8161 aaattctttg atatataatg attacaaagt tagactgtat tggaaaatgc acatacatga
     8221 attgatgaca atataagtga ttcccaaata taaatgaaag tataagctta ttttcactat
     8281 aaaaattttc actgtacaaa aacattaatg acatta
//
LOCUS       DBGJFF010000152         8173 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.155, whole genome shotgun sequence.
ACCESSION   DBGJFF010000152 DBGJFF010000000
VERSION     DBGJFF010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8173
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.155"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     regulatory      68..111
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            212..1180
                     /locus_tag="ACOIAF_003364"
     CDS             212..1180
                     /locus_tag="ACOIAF_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HGZ2670357.1"
                     /translation="MEKRKVIIDCDPGIDDSLAILLALNSPELEVIGITTCCGNVPAN
                     IGAENALKTLQMCSSLNIPVYIGEEAPLKRKLVTAQDTHGEDGIGENFYQKVVGAKAK
                     NGAVDFIINTLHNHEKVSIIALAPLTNIAKALIKDKKAFENLDEFISMGGAFRIHGNC
                     SPVAEFNYWVDPHGADYVYKNLSKKIHMVGLDVTRKIVLTPNIIEFINRLDKKMAKYI
                     TEITRFYIDFHWEQEGIIGCVINDPLAVAYFIDRSICKGFESYVEVVEDGIAMGQSIV
                     DSFNFYKKNPNAIVLNEVDEKKFMYMFLKRLFKGYEDIIDSVEGVI"
     gene            1182..1745
                     /locus_tag="ACOIAF_003365"
     CDS             1182..1745
                     /locus_tag="ACOIAF_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HGZ2670358.1"
                     /translation="MKRKICVKTITLIAFGISINIIGAFIAMGLRLPVYLDSIGTIMI
                     ASLLGPKYAVVTGVFGSLISGITFDVYSLYFAPVQISTGLLAGIAFKKNFLRGLKTPL
                     GVLLFAIPTSIISSIISAFLFGGMTSSGSSYIVQILKVLGLGDVFSVFVTQVFTDYGD
                     KLLAVVLVNLGLNAVPKTLKVSLTGGK"
     gene            1753..2442
                     /locus_tag="ACOIAF_003366"
     CDS             1753..2442
                     /locus_tag="ACOIAF_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HGZ2670359.1"
                     /translation="MDRYSEITNKNQREIVLLKGFPCIWGKCSFCDYIDDNSNLEEEM
                     NKLNLKVLKNVTGKYGVLEVINSGSCFELPKDTLEKIKCIIKEKNIKKLFLESHWSYK
                     NRLKEMREYFEIPVVFKIGVETFDNDFRNNILNKNANFKTPQDVKEYFDSPCIMVGIK
                     GQTKEMIDKDIEIILNTFEKATVNVFINNSSSIKRDEELVKWFVSKYKFLDENPNIEV
                     LYNNTDFGVGD"
     gene            2569..3297
                     /locus_tag="ACOIAF_003367"
     CDS             2569..3297
                     /locus_tag="ACOIAF_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HGZ2670360.1"
                     /translation="MMFDRGKWNKLDYNELLEYMMSIQDIKYRDFNKKLIPGTENIIG
                     IRVPNLRKLSKEISQGNWKEFLEVAQDTYYEEVRLQGMVIGNINSNFEETLYYVKRFI
                     PKIDNWSVCDGFCSDLKSMKKYKENMYDILKKYVYSKNPWEIRFALVMFLIYYVDDKH
                     IKEIFEYCNNIQSEEYYVKMGMAWLLSICFIKCEEETFLYIKNNNLDDFTYNKMLQKI
                     IESNRVDLEKKNIIRSMKRKTRRC"
     gene            3311..3985
                     /locus_tag="ACOIAF_003368"
     CDS             3311..3985
                     /locus_tag="ACOIAF_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGZ2670361.1"
                     /translation="MNNNEDRIDKFLELQSKGFKFAEIAQNLKISQSTLRTFLNKKGY
                     KSEKGKYILKDELNYNKSLDNRNLEEEKAKEYEKSKKNTISNRKKVSTKKKISNEKAL
                     SKSVEQIEFNDTGLKNAKPKQNVKPKKDRKINITQEDLDKLCEVYDWYLEVKDYNIKP
                     KKVSNKKDVHIDDEHIDELKTTRIRIDKEIWNDFERLCSNSNFEKHQILTQALKEFMK
                     KYKNLL"
     gene            4011..4847
                     /locus_tag="ACOIAF_003369"
     CDS             4011..4847
                     /locus_tag="ACOIAF_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670362.1"
                     /translation="MNKIVKKSISLMIILTIFIFMLTACEKDEQPDSVDIQTEDKNEI
                     KIDEGNAKVLNIGKSEIINIDEGDKVDTSTKVENNSTFNISNVELIYNEYDENKKIIS
                     SDSKALLDMTLMPGKVAYIECGHKTFVKSVEVYAYEYEAEGKIVYVNLKENTINIRNN
                     NIKLENSSQYEVLSTSELKKVNESNEGITYQVKVKNSSSKDLGNIILKTAEVNENGEY
                     LTVNRVPSYKILKPSEETDIDILCSTKAKSVEIVGYTYDDIKEKANVDIDLKSHKVKI
                     DK"
     gene            4863..5534
                     /locus_tag="ACOIAF_003370"
     CDS             4863..5534
                     /locus_tag="ACOIAF_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGZ2670363.1"
                     /translation="MKVLIVEDNKILLESVVEELNKHFETEKCEDGEEALYLVNQNIY
                     DLVILDLMLPNINGFDILKKMRVNNIDTPVLILTAKETLDDKVEAFTIGANDYLTKPF
                     YMEELVARVYAILRTNGKIKERNGLEFKSLYLDTLEKRVYIEKEEIKLQNKQFNLLEY
                     FVLNKGSILLKEQIYDRIWGIDSDATIEIVEVYVSNLRKKLSKYGYDKYIKTKRKVGY
                     IFDDK"
     gene            5524..6756
                     /locus_tag="ACOIAF_003371"
     CDS             5524..6756
                     /locus_tag="ACOIAF_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HGZ2670364.1"
                     /translation="MINKNVFTSTKNHLIKMYIIVVGSFLIIFSIFIYSYFRGLTYSG
                     IDSEINDELEYIVSQFKRTSFLNPIRLKDPKDMVYVYEDGRISYYTQNEYFDELLPDR
                     RLDKKNSFFKYTENGYTFRELNVDVGRYQIQIIRNIDSEMNSLRQLTSVLIIGILISV
                     IITYFVAVYLTRKALIPIETAWKNQAKFIQDASHELRTPITIVSSKLESMLKSPESTV
                     NDEVETIATAMKETRRLKKMITDLLSLTKEDSIVKVNLEEIDLEKLLEEISEDYIDIA
                     EFQEKRFVFNSKLKNKIIITDKNKLRQLILIFIDNAFKYTKLGDEIALELKEDIEDEV
                     TLLISDTGIGIKKEEIPLIFDRFFRSENVRNKDLEGSGIGLSIARMISLNLSIDINVT
                     SDVDIGTTFELSIPKKLK"
     gene            6906..7223
                     /gene="trxA"
                     /locus_tag="ACOIAF_003372"
     CDS             6906..7223
                     /gene="trxA"
                     /locus_tag="ACOIAF_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HGZ2670365.1"
                     /translation="MAKVINTSEFRSSVEGSKGVVVVDFFATWCGPCNMLGPVFAELG
                     EEMKDKARFVKVDIDESLEIAQQFNVSTVPTMIIFKDGKPVETLIGFMPKNKIEMQVK
                     SYL"
     gene            7240..8118
                     /locus_tag="ACOIAF_003373"
     CDS             7240..8118
                     /locus_tag="ACOIAF_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HGZ2670366.1"
                     /translation="MRYDIAIIGSGPAGLSAAINAKIRNKTIIMFGNDNLSNKLVKAP
                     SIDNYLGFYDISGDELKDKFKSHIDSMDISIENKRINNIYAMGEYFTIMSGNDMYEAT
                     TVILATGVEYTRPIKGEEEFLGRGVGYCATCDAPLYRNKKVAVIGYNEESKEEANFLS
                     ELTSKTYFIPMYKKDNLMRSSDNLDDSIEVIHDRPVQIDGDKLVNKVSFKENHIEVDG
                     VFVIKDSTAPSALVPGIEIDGIHIKVDNNMKTSIDGCFAAGDCVGKPYSYIKAAGQGQ
                     IAALNAVYYLDKLKRA"
BASE COUNT         3386 a          767 c         1458 g         2562 t
ORIGIN      
        1 aaaaattttt taacagtcta tagtttttaa gagtctatag taagtagcct aaaaatatga
       61 ttttacatct gtacttgttc gagaactcca agtataaaaa actaaggaat tatatagtgg
      121 cagtatataa ttaataccct tagacacacc tttttgaaag tggaaaaaag atgtgtagtt
      181 ctcctcgacc acaaatcaag gaggataaaa aatggaaaag agaaaagtaa taattgattg
      241 tgacccagga attgatgatt ctttggcaat tcttctggct ttaaactcac cagagctaga
      301 agtaattgga attaccacat gttgtggaaa tgttccagca aatataggtg cagaaaatgc
      361 actaaaaaca cttcaaatgt gttcttcact aaatattcca gtatatatag gagaagaagc
      421 accactaaaa agaaaacttg taacagctca agatacacat ggagaagatg gtattggaga
      481 aaacttttat caaaaggttg tgggagctaa agcaaaaaat ggagcagtgg attttataat
      541 aaatacttta cataatcatg aaaaagtatc aataatagca cttgcaccac ttacaaacat
      601 agctaaagca cttattaaag ataagaaagc atttgaaaat ctcgatgagt ttatatctat
      661 gggaggagca tttaggattc atggaaattg ctctccagta gcagagttta attattgggt
      721 agacccacat ggagcagatt atgtttacaa gaatttatct aaaaaaatcc acatggtagg
      781 tttagatgta actagaaaaa ttgtacttac tcctaatatt attgagttta taaatagact
      841 tgataagaag atggcaaagt atataactga aataactaga ttttatattg atttccattg
      901 ggaacaagag ggaataattg gctgtgtgat aaatgaccct ctagcagtag catactttat
      961 agatagaagt atatgtaaag gatttgaatc atatgtagaa gttgtagaag atggaatagc
     1021 tatgggtcag tctatagtgg attctttcaa tttctataaa aaaaatccta atgcaattgt
     1081 tctaaatgaa gttgatgaga agaaatttat gtacatgttt ttaaagaggc tttttaaagg
     1141 ttatgaagac attatagact ctgtggaagg agtgatatag tgtgaaaaga aaaatatgtg
     1201 taaaaactat tacacttata gcttttggta tttctataaa tataataggc gcttttatag
     1261 ctatgggatt aagattacct gtatacttag attcaatagg aactataatg atagcttcac
     1321 ttttaggacc taaatacgct gttgttacag gtgtgtttgg aagtttaata agtggaatta
     1381 cgtttgatgt gtattctctc tattttgcac cagttcaaat atcaaccgga ttacttgctg
     1441 gaatagcatt taaaaagaat tttttaaggg gtttaaaaac tccactagga gtacttttat
     1501 ttgccattcc aacatctata ataagttcaa taatttcagc atttttattt ggaggtatga
     1561 cctcttcagg ttcatcatat atagtgcaaa tcttgaaagt gttaggactg ggagatgttt
     1621 ttagtgtgtt tgtaacccaa gtatttactg attatggaga taagctgtta gcagttgttt
     1681 tagtgaattt aggattaaat gcagttccaa aaactttaaa ggtatcattg actggaggaa
     1741 aatagataga aaatggatag atatagtgag ataacaaata aaaatcaaag agaaattgtt
     1801 ctgcttaaag gatttccttg tatatgggga aagtgttctt tttgtgatta tatagatgat
     1861 aattctaatt tagaagaaga gatgaataaa ttaaatctaa aggttttaaa aaacgtaact
     1921 gggaaatatg gagttcttga agtcataaat tctggaagct gttttgaatt acctaaggat
     1981 actttagaaa aaataaagtg tattataaaa gagaagaata taaaaaaatt atttttagaa
     2041 agtcattggt cttacaaaaa taggcttaag gaaatgagag agtattttga aattcctgtg
     2101 gtatttaaaa ttggagttga aacctttgac aatgatttta gaaataatat tttaaataaa
     2161 aatgcaaatt ttaagactcc acaagatgta aaagaatatt ttgattcacc ttgtattatg
     2221 gttggaataa aaggacaaac aaaagagatg atagataaag atatagagat tatcctaaat
     2281 acttttgaaa aagcgacagt gaatgttttt ataaataatt cttctagtat aaaaagagat
     2341 gaagagttag tgaaatggtt tgttagtaag tataaattct tggatgaaaa tccaaatata
     2401 gaagttttat acaataatac agactttggt gtaggagatt agcattaatt tagtttaaat
     2461 ttaaaattga aaagtttctt atgtttttga aaaagcttct aatagttaga agcttttttt
     2521 gtagagtttt atgttacaat aagaaaaaag gaattaagga gcaattaaat gatgtttgat
     2581 aggggaaaat ggaataaatt ggattataat gaacttttag aatatatgat gtctatacaa
     2641 gatataaaat atagagattt taataaaaag cttatacctg gaacagaaaa tataataggt
     2701 attagagttc ctaatttaag aaagttatca aaagaaataa gccaagggaa ttggaaggaa
     2761 tttttagaag tagcacagga tacatactat gaagaagtta ggctacaagg tatggtaatt
     2821 ggcaatataa atagtaattt tgaagaaact ttatactatg taaaaagatt tatacctaaa
     2881 atcgataatt ggtcagtttg cgatggattt tgttctgatt taaagtctat gaaaaaatat
     2941 aaagaaaata tgtatgatat acttaaaaaa tatgtttatt caaagaatcc atgggaaatt
     3001 agatttgcat tagttatgtt tttaatatat tatgttgatg ataaacatat taaagaaata
     3061 tttgaatact gtaataatat acaaagtgaa gaatattatg ttaaaatggg tatggcatgg
     3121 ttgctatcaa tttgttttat taaatgtgaa gaggaaactt ttctctatat aaaaaataat
     3181 aatttagatg atttcactta taataaaatg ctacaaaaaa taattgaatc aaatagagta
     3241 gatttagaaa agaagaatat aataagaagt atgaaaagaa agacaagaag atgttgacgg
     3301 aggaatatta atgaataaca atgaagacag aattgacaaa tttttagaac ttcaaagcaa
     3361 aggatttaaa tttgcagaga tagctcaaaa tctaaagatt agtcaaagta ctttaagaac
     3421 ttttttaaat aaaaaaggat ataagtcaga aaaaggtaaa tacatattaa aagacgaatt
     3481 aaattataat aaaagtttgg ataatagaaa cttggaagaa gagaaagcta aggagtatga
     3541 aaaaagtaaa aagaatacta taagtaatag aaaaaaagta agcaccaaaa aaaagatttc
     3601 taatgaaaag gctttaagta aaagtgttga acaaattgag tttaatgata caggtttaaa
     3661 aaatgcaaag ccaaaacaaa atgttaagcc taagaaagat agaaaaataa atataactca
     3721 agaagattta gataagcttt gtgaagtata tgattggtat ttagaagtaa aagattacaa
     3781 tataaagcct aaaaaagtat ctaataaaaa agatgtacat atagatgatg aacacataga
     3841 tgaattaaaa acaacaagaa tacgtataga taaagaaata tggaatgatt ttgaaagatt
     3901 atgtagtaat tcgaattttg aaaaacatca aatacttaca caggcattga aagaatttat
     3961 gaaaaagtat aaaaatctac tataaaatac tactatatga ggtgcttaat atgaataaaa
     4021 tagtaaaaaa atcaatttct ctaatgatta tattaacaat atttattttt atgttgacag
     4081 catgtgaaaa agatgaacag cctgattcag ttgatattca aacagaagat aaaaatgaga
     4141 taaaaataga tgagggaaat gcaaaagtat tgaatatagg aaaatcagaa attataaata
     4201 tagatgaagg tgataaagta gatacatcaa caaaggtaga aaataattca acatttaata
     4261 tatccaatgt tgaattgata tataatgaat atgatgagaa taaaaaaatt atatcttcag
     4321 atagtaaggc tttgttggac atgactttaa tgcctgggaa agttgcttac atagaatgtg
     4381 ggcataaaac ttttgtaaag agtgtagaag tttatgcata tgaatatgag gcagagggta
     4441 agattgtgta tgttaattta aaagaaaata ctattaatat aagaaataat aatattaagc
     4501 tagaaaatag tagtcagtat gaggtactgt ctacatcaga acttaaaaaa gtaaatgaat
     4561 caaatgaggg aattacatat caagtaaaag taaaaaattc atcatcaaaa gatttaggaa
     4621 atattattct taagacagca gaagttaatg aaaatggaga atatttaacg gtaaatcgtg
     4681 taccatcata taagatttta aagccttcag aagagacaga tatagatata ttgtgttcaa
     4741 caaaggcaaa aagtgttgaa atagtggggt atacgtatga tgatataaaa gaaaaagcca
     4801 atgtggatat tgacttaaaa tctcataaag ttaagataga caaataaagg taggggatac
     4861 ttatgaaggt tttaatagtt gaagataata agatactttt agaaagtgta gtagaagaac
     4921 ttaacaagca ttttgaaact gaaaagtgtg aagatggaga agaagcttta tacttagtaa
     4981 atcaaaatat atatgattta gtcattttag acttaatgct tcctaatata aatggatttg
     5041 atatcctaaa gaaaatgaga gttaataata tagatacacc tgtacttata ttgacagcaa
     5101 aagaaacatt agatgacaaa gtagaagcat ttactatagg agcgaatgat tatttgacaa
     5161 aaccttttta tatggaagag ttagtagcta gggtatacgc tatattaagg actaatggaa
     5221 agataaaaga aagaaacgga ttagaattta aatctttata tttagataca ctagaaaaaa
     5281 gagtgtatat tgaaaaagaa gaaataaaac ttcaaaacaa acagtttaac ttgcttgagt
     5341 attttgtatt aaataaaggt tcaattttat taaaagagca gatttatgat agaatatggg
     5401 gaattgattc agatgcaact attgaaatag tggaagtata tgtaagtaat ctaagaaaaa
     5461 agctaagtaa atatggatat gataaatata ttaaaactaa acgtaaggta ggatatatat
     5521 tcgatgataa ataagaatgt atttaccagt actaaaaatc atttaataaa aatgtatatt
     5581 attgtagtag gtagtttttt aataatcttt tctatattta tatactcata ttttagggga
     5641 cttacatata gtggaattga tagtgagata aatgatgaac ttgaatatat agtctctcaa
     5701 tttaaaagaa cttcattttt gaacccaata agactcaaag accctaaaga tatggtgtat
     5761 gtatatgaag atggaaggat aagttactat actcaaaatg aatattttga tgagttactt
     5821 ccagatagaa gactagataa aaaaaattcg ttttttaaat acacagaaaa tggatatact
     5881 tttagagaat taaatgtaga tgtaggtaga taccaaatac agataattag aaatatagat
     5941 tctgagatga attctttaag acagcttaca tctgttctta taattggaat attaatttct
     6001 gtaataatta cttactttgt ggctgtatat ctcactagaa aagctttaat tccaatagaa
     6061 acagcatgga aaaatcaagc taagtttata caagatgctt ctcatgaatt aagaacacca
     6121 ataacaatag tttcatcaaa attagaaagt atgttaaaat ccccagaaag tacagtaaat
     6181 gatgaagttg aaacaatagc tactgcaatg aaggaaacta gaagacttaa aaaaatgata
     6241 actgatttat tgtctcttac taaagaggac tctattgtca aagttaattt agaagaaata
     6301 gacttagaaa agttattgga agaaatatca gaagactata ttgacattgc tgagtttcaa
     6361 gaaaaaagat ttgtgtttaa ttctaaactg aagaataaaa ttataataac agataaaaat
     6421 aaattaagac agcttatatt gattttcatt gataatgcct ttaagtatac aaaattgggt
     6481 gatgagatag cattagaatt aaaagaagat atagaggatg aggtaacttt acttataagt
     6541 gatactggta taggcataaa aaaagaagag atacccctta tatttgatag attttttaga
     6601 agtgaaaatg taagaaataa agatttagaa ggttctggaa taggactttc aatagctcgc
     6661 atgattagtt taaacttatc tattgatata aatgtaactt ctgatgtaga tattgggact
     6721 acatttgaat tgtcaatacc aaaaaagtta aaataataaa aatataaaaa attgttttta
     6781 gaaatgaaga atgggtatac taattagtat aaaatataaa aagaccttat aaaagtaaat
     6841 ttatatttat aaatttcata gtataattat atagggataa taaagtttaa taggaggatt
     6901 taaaaatggc aaaagttata aatacatctg aatttagaag tagtgttgaa ggtagtaagg
     6961 gagtagttgt agttgatttc tttgcaactt ggtgtggacc ttgtaacatg ttgggacctg
     7021 tatttgcaga attaggtgaa gagatgaagg ataaagctag atttgtaaaa gttgatatag
     7081 atgagagttt agaaatagct caacaattca atgtatctac agtaccaact atgattatat
     7141 ttaaagatgg aaaaccagta gaaactttaa ttggattcat gccaaagaac aaaattgaaa
     7201 tgcaagtaaa atcatattta taaaaaatgg gtgagtatta tgagatatga tatagctata
     7261 atcggcagtg gtccagctgg attgtctgct gctataaatg caaaaataag aaataaaaca
     7321 attatcatgt ttggtaatga taatttaagt aataagcttg ttaaagctcc ttcaatagat
     7381 aattatttag gtttttatga tataagtggt gatgaattaa aagacaagtt taaatcacat
     7441 atagatagta tggatatttc aattgaaaat aaaagaataa ataatattta tgctatggga
     7501 gaatacttta caataatgtc tggaaatgat atgtatgaag ctactactgt aatccttgca
     7561 actggggtag agtatactag accaatcaag ggtgaagaag aatttctagg aagaggtgta
     7621 ggctactgtg ctacatgtga tgcaccactt tatagaaata aaaaggtagc agttatagga
     7681 tataatgaag aatctaaaga agaagcaaat tttttaagtg aactcacttc aaagacttat
     7741 tttataccta tgtacaaaaa agataatctt atgagaagtt cagataacct agatgattct
     7801 attgaagtta tacatgacag accagtacag atagatggag ataaacttgt aaacaaagta
     7861 tcttttaaag aaaaccacat tgaagttgat ggagtattcg ttataaaaga tagtacagct
     7921 ccaagtgcat tggttcctgg aatagagatt gatggcattc atattaaagt tgacaataat
     7981 atgaagacta gtatagatgg atgctttgct gctggagatt gtgttggaaa accctattct
     8041 tatataaaag cagctggtca agggcaaata gcagctttaa atgctgttta ctatttagat
     8101 aaattaaaga gggcttaata tttatattga ttaacttttt aaagatattt ataaaaaact
     8161 gtcttgaaat aat
//
LOCUS       DBGJFF010000153         8087 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.156, whole genome shotgun sequence.
ACCESSION   DBGJFF010000153 DBGJFF010000000
VERSION     DBGJFF010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8087
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.156"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            <1..667
                     /locus_tag="ACOIAF_003374"
     CDS             <1..667
                     /locus_tag="ACOIAF_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670367.1"
                     /translation="SFRYESNINNTRINESMVKKNETVGKDTYLSNIMKQKQELNDRI
                     RDLKYRQEVYTKKINDAIKNLCKSEIRETTNNFSNIEIGIKNSIIEDKNKSTMLDENS
                     TYLNTNDEKESLITKESNEKIEEEILNDEKLEELEQKKDYKEDSNKKEKVSEDLSLVG
                     KTREELENMLKNFINLTQEEIMKLESRIEKLDKNAEEYKQNSKTNIFDKTDEQKKHIN
                     VLI"
     gene            845..1735
                     /locus_tag="ACOIAF_003375"
     CDS             845..1735
                     /locus_tag="ACOIAF_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670368.1"
                     /translation="MKKNILTTMITMIIILMMSMTVMAEPYQEDETSSLKKQSANERN
                     QTNVGTMDGRVQTNAISKGANYDPKYPLKGMLTQLGLNTTGKEGWDLLYGGNPNGVEL
                     RADGKYYYINKGMEGLGFYAAALSGGSYQRIGFGKLDPIEVIADGNWISYKQAQDTLT
                     VIRNFLNSFDWRNASEMERANRAAKLVTEAKYADSKYCNIVYGNLVDKRGVCGSFASS
                     FHLLTRLMGMDSLSILNPSLNHAWNYIQIDGKWYRFDGSEISAFGGSLDFDYRKLKDA
                     TREMTTYYDAKALSILGFNQ"
     gene            complement(2136..3092)
                     /locus_tag="ACOIAF_003376"
     CDS             complement(2136..3092)
                     /locus_tag="ACOIAF_003376"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HGZ2670369.1"
                     /translation="MENNTIQKQLRDLSKKIWHIYILNPEEENLKFIISLLDDNLALI
                     GTGKHEFYETLEEFIIGLEKDQTESEQIDFEIIDEWYSCTKVTDEAFIVYGTLWVIEK
                     DYLEKDIYVEMDTRFTMVYRVTDDMIKLIHVHHSIPNFDQMKDEYYPKTITEKAQEAL
                     AMANEFKLKSEKDLMTGLYNRHFFEVHIKEMLKTVSHGYFYIFDLDNFKQVNDIFGHV
                     RGDEILIHFSNILTKVFVNDAIIGRLGGDEFAVFEYSNQSKENVENKAKKVLELCLES
                     NSCSIGISKLENNSDTFQSLYKQSDYALYYSKRNNKGNFSWF"
     gene            3795..4790
                     /locus_tag="ACOIAF_003377"
     CDS             3795..4790
                     /locus_tag="ACOIAF_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGZ2670370.1"
                     /translation="MSIIELNELTKSYRRFEKKAGFMGSVKSLFNRKYTEKTAISSFD
                     LKIEEGEFVGLIGQNGAGKTTLIKMLTGIISPSSGEISVMGYYPNKLENNFKKQYAVV
                     MGQKSQLFFELTPADTFLLFKELYDISNEEYKKNLDYFIELFEVSDYLNVQVRTLSLG
                     ERMKMELIVALLHNPKILFLDEPTIGLDAIAQKQIRYFLKEVNETKGTTIILTSHYME
                     DIKSLCSRSIVVENSRKIYDGSTEKLFNHFQKYKKITVSFSNETNIVFPKDCKVVEQT
                     NHKISVIVPKERGNETIKWIFDKYDVKDVMIEEEDIGSIVERIYKKGSDEYYEQL"
     gene            4777..5631
                     /locus_tag="ACOIAF_003378"
     CDS             4777..5631
                     /locus_tag="ACOIAF_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2670371.1"
                     /translation="MSNYNEASSNYSMFYKFKQSIRRYYEVAKINFKVQLIWRFDVVV
                     NMVFTIVKILFAYILWKAIFGENKIVSGFTFNSMLSYYIISSFLAGIDMSKSTSEEIC
                     NRVKLGTFSKYMIIPINVQNYFIAKNFGMTCFYFVFNMISAFVWIFIFKIDFAFTSNP
                     VQILISILMIFLGFIFMMKLHFFIGILSFKFLEISFFRMIIENIVEFITGTMIPLVLL
                     PETIVTVMKVFPFYYTTYLPSMLLIGRNSNEAIQGLIILALWVLFFSVVNKVMYKRMR
                     TLFDGVGI"
     gene            5628..6422
                     /locus_tag="ACOIAF_003379"
     CDS             5628..6422
                     /locus_tag="ACOIAF_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HGZ2670372.1"
                     /translation="MMKKNLKIIFMLLKMKLSKMMVFRFDFFGAFFVDSSLFILQLLM
                     FNSIYSHVDSIGGWQQGEMLIFIGTFSLINAINMTIFFFGIYDIPRKIQEGELDYYIT
                     KPLDPLFRLTFENINIGSSPLILASILIVLYGISELNSEITLVMIIIYIFMVLLMTLL
                     FYDVCIILRTVSFFVISSSAIMRLEDNLLPMNMKIPGVIYEGIFKVLFYLILPYGIMS
                     TIPTQILAGTITINGLIYSILIVFLFTLFMWRFWKLGMRHYKSASS"
     gene            6707..8086
                     /locus_tag="ACOIAF_003380"
     CDS             6707..8086
                     /locus_tag="ACOIAF_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HGZ2670373.1"
                     /translation="MSDVYYSENHEQDNLYEQDNLLAQRFNLFTLLKFAFPTIFMMIF
                     MGLYTIVDTIFVSRFINTNALSAINIVCPIINLIVGIGTMLATGGSAIVARKMGNGDI
                     EGANKAFSLIVLSGVVIGVIITGIGIVFMDYIIIWLGASEVLIKYCRSYLFIILVFAS
                     ANILQVLFQCLFVTAGKPQFGLWLVLGAGCANIILDYVFIVLLQMGISGAALATGVGY
                     LIPTISGMVFFSKNKGLLKFCIPRLDKRILFEACFNGSSEMVGQLSTAVTTFLFNAMM
                     MKLIGEDGVAAITIIIYSQFLLTSLYIGFSMGVAPIISYNYGSENNTQLNHVFKICIL
                     FISVASLLVFIFSISCSPIIVETFSQRGSRVYEIARSGFYIFSFSFIFCGYNIFTSSM
                     FTALSNGKVSAILSFLRTFGFITIGLLILPNILNINGIWLAVPSAEILTLFLTVFCIF
                     KYKNRYKYM"
BASE COUNT         2992 a          870 c         1270 g         2955 t
ORIGIN      
        1 ttcatttaga tatgaaagta atataaataa tacaagaata aatgaaagta tggttaagaa
       61 aaatgaaact gtaggaaaag acacttattt atctaatatt atgaaacaaa agcaagaact
      121 taatgataga attagagatt taaaatatag acaagaggtt tatactaaaa aaataaatga
      181 cgcaattaag aacttatgta aatcagaaat aagagaaaca actaataact tttctaatat
      241 agaaataggt attaaaaata gcattataga agataaaaat aaaagtacaa tgttagatga
      301 aaattcaact tatctaaata caaatgatga aaaagaatct ttaatcacta aagagtctaa
      361 tgaaaaaatt gaagaagaaa tattaaatga tgaaaaatta gaagagttag aacaaaaaaa
      421 ggattataaa gaggattcta ataaaaaaga gaaagtatct gaggacttat ctttagtagg
      481 taaaactcgt gaagagcttg aaaatatgct taaaaatttt ataaatttaa cacaagaaga
      541 aataatgaaa cttgagtcga gaatagaaaa gttagataaa aatgctgaag aatacaaaca
      601 aaattcaaag actaatatat ttgataaaac agatgaacaa aaaaaacata taaatgtact
      661 gatttaaaca atatgtttta aaaaaataag tttattatat aatgaaaata taaaggtgca
      721 atttcaatac acttctatta aaccaataat ttatgaatca gacttaattt caatttacta
      781 ataaccatat atttatgagt taataatttt atgttttaaa aagaattaaa taagggggag
      841 tagtatgaag aagaatatat taactactat gattaccatg attattatat tgatgatgag
      901 tatgacagta atggcagagc catatcaaga agatgaaaca tctagcttga aaaaacagag
      961 tgcaaatgaa cgtaatcaaa ctaatgtagg cacaatggat ggtagagtgc aaacaaatgc
     1021 aataagtaaa ggtgctaatt atgaccctaa atatcctctt aaaggaatgt taacacaact
     1081 tggtcttaat acaacaggta aagagggatg ggatttactc tatggaggaa atcccaatgg
     1141 agttgagctt cgagcagatg gtaaatatta ttatataaat aagggtatgg aagggctagg
     1201 attttatgcg gcagcattgt caggtggctc atatcagaga ataggctttg gaaaacttga
     1261 ccctatagaa gtaatagctg atggaaattg gatttcttac aaacaagctc aagatacact
     1321 tacagtaata aggaattttt taaatagttt tgactggaga aatgcatctg agatggaaag
     1381 agctaataga gctgcaaaat tggtaaccga agcaaaatat gcagactcta aatattgtaa
     1441 catagtctat ggtaatctgg ttgataaacg tggtgtatgt ggaagttttg caagtagctt
     1501 tcatttatta acaaggttaa tgggaatgga ttcactatct atattaaatc catcattaaa
     1561 tcatgcttgg aactatatac aaattgatgg gaaatggtat aggtttgatg ggtctgaaat
     1621 atcagctttt ggtggatcat tagactttga ttaccgtaaa ttaaaggatg caacaagaga
     1681 gatgactact tactatgatg ctaaggcatt gtctatatta ggatttaatc aataaaaata
     1741 gggaaggctt cttatggagc ctttttttat tgatttgaaa tttaataagg aattttatat
     1801 gaaaatttaa tcaataaaag caaaaaaggc tctttataaa gcctttctaa gtgttttttt
     1861 aagtaaaatt ttttatatca acatcagttt taatcaaaaa atataacatt ttggacttac
     1921 ttttaaaaat aaaattatta aaatataata ttaatcatgt taataatgtt tttagtttat
     1981 tatattctat aatagtggtc atgttgacta ttgtagtttt attaaaaata gttggtttat
     2041 ataaatatat gcaaagatag cttgttgttt actttactta ttagtatata ggacaagata
     2101 ctctaataaa tttgcctatt ttaatatctt ttgtactaaa accaactaaa gtttccttta
     2161 ttatttcgtt ttgaataata caaagcataa tccgattgtt tgtataaaga ctggaaagta
     2221 tcagagttat tttctaattt agatatacct atagaacaag aattactctc taggcataat
     2281 tctaaaacct tctttgcctt attttcaaca ttctcttttg attgattact atattcaaaa
     2341 acagcaaatt catctccacc aagtcttcca ataatagcat catttacgaa tactttagtc
     2401 aaaatatttg aaaaatgtat tagtatttca tctcctcgaa catgaccaaa gatatcattt
     2461 acctgtttaa aattatctaa atcaaagatg taaaagtatc catgtgaaac agttttaagc
     2521 atttctttta tgtgtacttc aaaaaaatgt ctattgtata gtccagtcat aaggtctttt
     2581 tctgatttta gtttaaattc atttgccatt gctagtgctt cttgtgcttt ttcagttatt
     2641 gttttagggt aatactcatc tttcatttgg tcaaagtttg gaatagaatg gtgaacatgg
     2701 ataagtttaa tcatatcatc tgtaactctg tataccatag taaatcgagt atccatttct
     2761 acatagatat ctttttctag gtaatctttt tctattaccc aaagagtacc atatactata
     2821 aaagcttcgt ctgtgacttt ggtacatgaa taccattcat ctataatctc aaagtctatt
     2881 tgctctgatt ctgtttggtc tttttcaaga cctataataa attcttctaa tgtttcgtaa
     2941 aattcatgtt ttcctgttcc aattaatgca aggttgtcat ctaataagga aataataaat
     3001 ttaaggtttt cttcttctgg atttaaaata taaatatgcc aaattttttt tgataaatct
     3061 cttaattgtt tttgtattgt attattttcc atagattacc cccttaagat ttacagttta
     3121 tattttaatg tattataagt tgaactctta ttacaatatt attataatat agaactaaag
     3181 aaattacatc ctgtataggt aaaagtatat cttttacaat aaaaatatta cagtaaaata
     3241 accagtttta atatacgtat tatattttta atttataaac gaatattata aatgaaattt
     3301 aaattcaacc ctgaagaaat aagctaaatg tattagtata taaatccata cttgaacata
     3361 aataaccttt atgatatgat atcaataata atattatgag aggagtgcct tgatgtcgga
     3421 gttatataat ggtaaccaac aattaaatag ttggagacac atatattctg tataatcaga
     3481 aatttcaagt gtatttttaa tacatttaat ttttgtgtct tattctgcct ttatagataa
     3541 ctgacatatg acaacaaata gtatttaaaa tatatattac tttgttaaat tatggtgtag
     3601 taggctttaa tctgctacac ttttattaat tttgtatagt attattttga taactatttt
     3661 gaaatagaga gcattaattt ttctagtcca caggagtttt ctgtttttgt gctgttaaat
     3721 ataagcacag aaaaaggcag atatatacct gtggactttt tttatataaa ttatgaaata
     3781 tggaggtagt taaaatgagt ataattgaac ttaatgaact gactaaatct tatagaagat
     3841 ttgagaaaaa ggcaggattt atggggagtg taaagtcttt atttaataga aaatatacag
     3901 aaaaaacagc tatatcgtca tttgatttaa agatagaaga aggagaattt gttggattaa
     3961 taggtcaaaa tggagctggc aaaactactc ttattaagat gttaactggt attatttcac
     4021 cgtcttctgg agaaatatct gtaatgggtt attaccctaa caaattagaa aataatttta
     4081 aaaaacaata tgctgttgtt atgggccaaa aaagtcaact attctttgaa ttgacaccag
     4141 cagatacgtt tcttttattt aaagaattat atgatatatc taatgaagaa tataaaaaaa
     4201 acttggatta ctttatcgaa ttatttgagg tatctgacta tttaaatgtt caagttagga
     4261 cgctttcttt aggagaaaga atgaaaatgg aactaatagt agcccttcta cataatccaa
     4321 aaatattatt tttggatgaa ccaactatag gcttagatgc tatagcccaa aaacaaatta
     4381 ggtatttcct aaaagaagta aatgaaacaa aaggaactac tattatactt acttctcatt
     4441 acatggaaga tattaagagt ctctgtagta gaagtattgt tgtagaaaat agccgtaaaa
     4501 tatatgatgg ttctacagag aaactattta atcattttca aaaatataaa aaaataacag
     4561 ttagtttttc caatgaaact aacattgtat ttccaaaaga ttgtaaagtt gttgaacaaa
     4621 caaatcataa gatatcagta attgtgccaa aagaaagagg aaatgaaacg attaaatgga
     4681 tttttgataa atatgatgtt aaagatgtaa tgattgaaga agaagatata ggtagtattg
     4741 tagagagaat atataagaaa ggaagtgatg agtattatga gcaactataa tgaagcaagt
     4801 tcaaattatt caatgtttta taaatttaaa cagagtatta gaaggtatta tgaagtagcc
     4861 aaaattaatt ttaaagtgca acttatctgg agatttgatg tggtggttaa tatggtgttt
     4921 acgattgtaa aaattttatt tgcttatatt ttatggaaag caatttttgg tgaaaataaa
     4981 atagtctctg gttttacatt taactctatg ttgtcttact atataataag ctcattttta
     5041 gcaggtattg atatgtcaaa gagtaccagt gaagagatat gtaatcgtgt aaaattgggc
     5101 accttttcaa agtacatgat tattccaatt aatgttcaaa attattttat tgctaaaaat
     5161 tttggaatga catgttttta ttttgtattt aatatgattt ctgcatttgt atggattttt
     5221 atatttaaaa tagattttgc ctttacatca aatccagttc aaatattaat ttcaattttg
     5281 atgatttttc tagggtttat ttttatgatg aaactccact tcttcattgg gattttgtca
     5341 tttaaatttc ttgagattag tttttttaga atgataattg aaaatattgt tgaatttatt
     5401 acaggaacaa tgataccact tgtactctta ccagaaacta tagtaacagt tatgaaggta
     5461 tttccatttt attatacaac atatttgcca tctatgttac taataggtag aaacagcaat
     5521 gaggcaatac aaggattaat tattctagca ttatgggttt tatttttttc tgtagtaaat
     5581 aaagttatgt ataaaagaat gagaacatta tttgatgggg tgggtatatg atgaaaaaaa
     5641 atttgaaaat aatatttatg ttattaaaaa tgaaattatc aaagatgatg gtatttagat
     5701 ttgatttttt tggagcattt tttgtagata gttcattatt tatattacaa cttctaatgt
     5761 ttaatagtat atattctcat gtagactcaa tagggggatg gcaacaagga gagatgttaa
     5821 tatttatagg aactttctca ttaataaatg cgataaatat gacgattttc ttttttggta
     5881 tttatgatat tccacgaaaa atccaagaag gagaattaga ctactatatt acaaaaccat
     5941 tagacccact atttaggtta acatttgaaa atattaatat tggttcatca ccattaattc
     6001 tagcaagtat acttattgtt ttatatggaa tatctgagtt aaactcagag ataactttag
     6061 ttatgataat aatatatatt ttcatggtgt tattgatgac attacttttt tatgatgtat
     6121 gtatcatact tagaacagtt tcattttttg ttatatcatc ttctgcgata atgagattag
     6181 aagataattt attgccaatg aatatgaaaa ttcctggtgt aatatatgaa ggtattttta
     6241 aagtattatt ctatttaatc ttaccatatg gaattatgtc gacaattccg actcaaattt
     6301 tggctgggac aataacaatt aatggattaa tatactcaat tttaattgta tttttgttta
     6361 cattgtttat gtggagattt tggaagttag gtatgagaca ttataaaagt gcaagtagtt
     6421 aataaattag taaaaataga caatgtataa ctatatataa atacgtttca tatgacacta
     6481 aactatgtga acaagtattt gcgctaaagg cactgaaagc tataaaatga ttaaagtaag
     6541 gatttaaaag tttctttagt agaaatataa ttgtgtaaaa aagcaagatt aattaaaact
     6601 atatccaaat aattagatat aattcttatg cagaaaggag gtctgtaaat gtctaattgt
     6661 ttggaatata ttttaaaaat aattaatgta cagaaaaata aacaaaatga gtgatgtata
     6721 ttattcagaa aatcatgaac aagataatct ttatgagcaa gataatttac tggcacaaag
     6781 atttaattta tttactctat tgaagtttgc atttcctact atttttatga tgatttttat
     6841 ggggttgtat acaatcgtag atactatatt tgtatcaaga tttattaata caaatgctct
     6901 atcagcaatt aatattgtat gtccaattat taatcttata gttggaattg gaactatgct
     6961 tgccactgga ggaagtgcta ttgtagcacg taagatggga aatggtgata ttgagggagc
     7021 aaataaagct ttttctttaa tcgtattatc aggagttgta attggggtaa taattactgg
     7081 tataggtatt gtatttatgg attatatcat tatatggtta ggagcaagtg aagttttaat
     7141 taaatattgt cgttcatatc tttttattat cttggttttt gcttctgcaa atatactaca
     7201 agtattattt caatgtttat ttgtaacagc tggaaagcct caatttggat tatggctagt
     7261 gttaggtgct gggtgtgcta atataattct tgactatgtg ttcattgttc tgttacaaat
     7321 gggtattagt ggagcagcac ttgcaactgg agtaggatac ctaattccta ccatttcagg
     7381 tatggttttc ttttcaaaaa ataaagggtt acttaaattt tgtataccta gactagataa
     7441 aagaatatta tttgaagctt gttttaatgg ttcatctgaa atggtgggtc aactatcaac
     7501 agcagttact acttttttat ttaatgctat gatgatgaaa ttaattggtg aagatggggt
     7561 tgcagctatt acaataatta tttattctca atttctttta acttcgcttt atataggttt
     7621 ctcaatgggt gttgcaccaa taataagtta taactatgga agtgaaaata atacgcagtt
     7681 aaatcatgtt tttaagattt gtatattatt tatttcagta gcatctttat tggtatttat
     7741 cttttctata tcatgttcac caatcattgt agagactttt tctcaaagag gaagccgagt
     7801 ttatgaaatt gcaaggtccg gattttatat attttctttt agttttattt tttgtggata
     7861 taatattttt acctcatcta tgtttacagc attatcaaat ggaaaggtgt ctgcaatatt
     7921 atcttttcta cgtacatttg gatttataac tataggttta ttgatattgc caaatatatt
     7981 aaatataaat gggatatggt tagcagtacc aagtgctgag attttaacct tatttttaac
     8041 tgttttttgt atatttaagt ataagaatag atataaatat atgtaga
//
LOCUS       DBGJFF010000154         7990 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.157, whole genome shotgun sequence.
ACCESSION   DBGJFF010000154 DBGJFF010000000
VERSION     DBGJFF010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7990
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.157"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            151..918
                     /locus_tag="ACOIAF_003381"
     CDS             151..918
                     /locus_tag="ACOIAF_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaP domain-containing protein"
                     /protein_id="HGZ2670374.1"
                     /translation="MQIIDININNIDSEHICCSISEKKGENCVFSKKAWMKNNFNKGL
                     VFKRFDVRGKAFIEYIPAENAWCPITADNYIFINCFWISGKFKGQGYSNLLLQECIDD
                     AKSKGKYGLVVLSSSKKMPFLSDTKYLKYKGFCLADTSEPNYELLYFPFTDNAPIPCF
                     KNCVKKRKLNENGIVLYYSNQCTHTEKYAPILKKIANSRGIDVNLIKFETAKEAQNAP
                     APFTTYSLFFNGEFVTNEILSEKKIEKFLDTNIKKFN"
     misc_binding    1136..1349
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1603..2637
                     /gene="argC"
                     /locus_tag="ACOIAF_003382"
     CDS             1603..2637
                     /gene="argC"
                     /locus_tag="ACOIAF_003382"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HGZ2670375.1"
                     /translation="MNKIKVGIIGATGYVGAELIRLLINHDKVEVTAIGSNSYVGKDI
                     VDIYPSIGYKNNMICIENEKVIDMCDVVFTALPHGVSEKFVIKAIKSKKKVIDLGADF
                     RIKDEEVYSKWYGVSFIDKILHKKAVYGLSEIYKEDIKDADIIANPGCYPTSISLPLM
                     PLLSSKLIKNNNIIIDSKSGLTGAGRELSISSHFTEVNENITAYKIGKHRHTPEIEQN
                     LSESCKEKVSVVFTPNLIPVNRGILSTIYCTKEDNISINDIHRKLTDYYEFKEFIEVL
                     PLDKVASLKNVRFSNKCVISLHENGDTLIICSAIDNMIKGAAGQAIQNMNIIFGIEEN
                     TGLKNIAPSF"
     gene            2678..3895
                     /gene="argJ"
                     /locus_tag="ACOIAF_003383"
     CDS             2678..3895
                     /gene="argJ"
                     /locus_tag="ACOIAF_003383"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HGZ2670376.1"
                     /translation="MEIIKGGVTVSEGFFASGIHCGLRKNKEKRDLALVYSDVLCDAA
                     AVVTQNKVKGNPVYVTQEHIKNGKAQAIIVNSANANTFNGKEGLINAYKMAKFTSDKL
                     NIKESDVLVASTGVIGKPLNIDLIEENMDELVNNLSKQGHIGAREAIMTTDIIKKEIA
                     VAIMYGDKKITIGGMAKGSGMIHPNMATTLGFITTDANIDGVLLKEALKIAVDKSFNR
                     VSVDGDTSTNDMVMILANGKAKNDRINKKDEHYQVFLSALTYVCIELAKLVAKDGEGC
                     TKLIECYINGALSEEHAVKLAKTVISSSLVKTAVFGADANWGRILCALGYAGEEIDME
                     KVDIIFESMKGYIEVCKNGNGLDFNEEKAKKILEDDEISILVDLNMGNARGNAWGCDL
                     SYDYVRINGSYRN"
     gene            3910..4770
                     /gene="argB"
                     /locus_tag="ACOIAF_003384"
     CDS             3910..4770
                     /gene="argB"
                     /locus_tag="ACOIAF_003384"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HGZ2670377.1"
                     /translation="MINIEKANTLIEALPYIEKHQGKTIVVKYGGSAMKKDGLKESVM
                     EDLVLMSYVGINIVLVHGGGAEINKMLAKVDIESKFVNGLRYTDEETMEIVKMVLAGK
                     VNKDLVNKIHTKGGKAVGLCGIDNNMILCDPYKNYELGFVGEIKKVNVELIESCLKSG
                     YISVIATIGVGDDGETYNINGDTAASAIAKELNADKLILLTDVPGLLREPDEEKSLIT
                     EVILEDVDKLFEEGIITGGMIPKIQGCVDALNNGVNRVHILDGRVPHSIITELFTDSG
                     IGTLIRKENE"
     gene            4800..5987
                     /locus_tag="ACOIAF_003385"
     CDS             4800..5987
                     /locus_tag="ACOIAF_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HGZ2670378.1"
                     /translation="MNNNNTISKWNEYFIDTYNQPNFVIDYGEGSCFFDTNGNKYIDF
                     TSGYGVSSLGYSNGNLKNALKEQVDKLLHTSNLYFNEPVLYSGEKIINSSGMAKVYFC
                     NSGTEANETAFKIARKYSSDKYGNGRGTIISLKDSFHGRTMMSLMATGMDKYHKYFYP
                     LPEGFKYIERNNIEDLKNNIDSTVCAIILEAIQGEGGVNVLEKDYVLELVKICQEKDI
                     VVIFDEVQCGIGRTGKLFGYEYFDVKPDIVTVAKGLGAGIPVGGVLVNKKLSKVLGKG
                     DQGTTFGGNLLAMVATSVVLDEISKDGFYNEVLEKGNYIRKSIESFNNKVVLKTKGIG
                     LMIGIETNIESSIIEEKARKKGLLILTAGKNVLRFLPPLTISYKEIDEALEILKDILL
                     ETN"
     gene            6097..7119
                     /gene="argF"
                     /locus_tag="ACOIAF_003386"
     CDS             6097..7119
                     /gene="argF"
                     /locus_tag="ACOIAF_003386"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HGZ2670379.1"
                     /translation="MNNISLKGKSFLTLKDFTKEEIRYLLTLSSYLKTKKKVGIKGDL
                     LQGKNIALLFEKTSTRTRCSFEVAAHDEGAHVTYLGPNDCHMGKKESVSDTAKVLGRF
                     YDGIEFRGFKQETVESLAKYSGVPVWNGLTDEYHPTQILADFLTVIENVDKDLNKVKF
                     VYVGDARNNMGNSLMIGCAKMGIHFVALAPKELWPNEELVKEMKEIAKKSKGDIMLTE
                     NIDDVKGADVIYTDVWVSMGEEEQYEARINQLKDYQVNMDMIKKTENENVIFLHCLPA
                     FHDLETKVAQEIYEKFGLSELEVTNEVFNSKYSKVFEEAENRMHTIKAVMVSTMTDVN
                     MNNNLV"
     gene            7246..7863
                     /locus_tag="ACOIAF_003387"
     CDS             7246..7863
                     /locus_tag="ACOIAF_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4878 domain-containing protein"
                     /protein_id="HGZ2670380.1"
                     /translation="MKKIILALTTFLLAISLTACSSAKPEDTIDSFFNSAKKFDFEGM
                     NKVMENNDEKYKDILKELDTKDPNAQYVLDYLKQNASKITYTIKDSEVKGDKATIKVE
                     CKFIDSTPLLQEIVAEAFTKMIGMSFSGQDLTDEKTTEMLVSIMKEKQKSIKETYVTK
                     NVEFECSKKDNKWIISSVNDAVADVLLSNLVTAGQEFSNSMNTLE"
BASE COUNT         3257 a          756 c         1491 g         2486 t
ORIGIN      
        1 atatgcaatt tgataattca aataatctaa aaataatatt atgtaaaata aaaatataga
       61 tgacataatg ttgttatatt caaaatgtta aaataaaaaa tatatagaat atatttaaaa
      121 attattatgt aattagaaaa ggagataagt atgcagatta ttgatataaa tataaataat
      181 attgacagtg aacatatatg ttgttcaatt tctgaaaaaa aaggagaaaa ttgtgttttt
      241 tctaaaaaag cttggatgaa aaataatttt aataaaggcc tagtctttaa acgatttgat
      301 gtaagaggta aagcttttat agaatatatt ccagccgaaa atgcatggtg tcctattact
      361 gcggacaatt atatatttat taactgtttt tggatatctg gaaaatttaa gggtcaagga
      421 tattctaatc tattgctaca agagtgtatt gatgatgcta aatcaaaagg taaatatgga
      481 ttagttgtgc tttcgtctag taaaaaaatg ccttttttat ctgatactaa atatctaaaa
      541 tataagggct tttgtttagc agatacatca gaaccaaatt atgagctatt atattttcct
      601 tttactgata atgctcctat tccatgtttt aagaattgtg taaaaaaaag aaaattaaat
      661 gagaacggta tagttttata ttacagtaat cagtgtacac atacagaaaa atatgctcct
      721 attttgaaaa aaattgcaaa ttctagaggt attgatgtaa atcttataaa attcgaaaca
      781 gcaaaagaag cacaaaatgc tcctgcacca tttactacat acagtctttt ttttaatggt
      841 gaatttgtaa ccaacgagat actttctgag aaaaagattg agaaattttt agataccaac
      901 attaaaaaat ttaactaaaa agagatgtaa atattgtatt ttgaaaatat attattttat
      961 aatttaatat attttggatg ttttttaaat tgacgaatga aatataaaaa ttatatcgca
     1021 atttagatac aaaatgctgg aaattaacca tatagaaaat atttccaaaa atttaaaaat
     1081 tatattgaca aagttaaaaa aataaactat catataaaac aataagaaaa taaattgaaa
     1141 tcctgtgaaa aagagagtag cataaagcta gttgatagag agctgggaat ggtggaagcc
     1201 tagtaacgaa ttttattgtg aatagagctt tggaggagat taccagaagt aatagtatgg
     1261 taattggtga atctcgcctt aagagattag agtgggtaag attatcttat caattagagt
     1321 ggtaacgcgg gtgtatattc gtctcttggt ctttgaccaa gaggcttttt taattttaaa
     1381 tgtacatttt gtagatactt tttaatttga gttttaatga ttattgtaat tttaccaata
     1441 agagtggtac cgcggaaaat cgcctcttag tatttataat gctaaggggt tttttgtttt
     1501 aattagagta ttttaatcta cgtattatca aatgattatc atatggtaaa gtggaaagat
     1561 aggattttaa gactatttga atataaaaat ggagggaaca aaatgaataa aataaaagtt
     1621 ggtattattg gtgcaactgg atatgtcggg gcagagttga ttagattatt aataaatcat
     1681 gataaagtcg aagtgacagc gataggttca aattcgtatg ttggaaaaga tatagttgat
     1741 atatatccaa gtataggata taaaaataac atgatatgta tagaaaatga aaaagttata
     1801 gatatgtgtg atgttgtgtt tacagccctt ccacatggag ttagtgaaaa gtttgtaata
     1861 aaggcaatta aatcgaaaaa gaaagttata gatttaggtg ctgactttag aataaaagat
     1921 gaagaagtat atagtaaatg gtatggagta tcttttatag ataaaatctt acataaaaaa
     1981 gctgtatatg gattaagtga aatatacaaa gaagatataa aagatgcaga tataattgca
     2041 aatcctggat gttatccaac tagtatttct ttaccattga tgccattact aagttctaaa
     2101 ttgataaaaa ataataatat aataattgat tctaaatcag gtcttacagg tgctggtaga
     2161 gaattatcga ttagtagtca ctttacggag gttaatgaaa atatcactgc atataaaata
     2221 gggaaacaca gacatactcc agaaatagaa caaaatttaa gtgaatcctg caaagaaaaa
     2281 gtaagtgtag tttttacacc aaacttaatc ccagtaaata ggggaatctt atcaactata
     2341 tattgtacaa aagaagataa tatttctata aatgatatac atagaaaatt aactgattat
     2401 tatgaattta aagaatttat agaagttctt ccattagata aggtagcttc tttaaagaat
     2461 gttaggttct caaataaatg tgtaatatca ttacatgaaa atggagatac attgataatc
     2521 tgttcagcta tagataatat gattaaggga gcagcaggtc aagctataca aaacatgaat
     2581 ataatatttg gtattgaaga aaatacaggt ttaaaaaata ttgcaccatc attttagaaa
     2641 taaaaaatac taaactcata taattaggag gataaatatg gagattataa agggtggagt
     2701 tacagtttca gaaggatttt ttgcttcagg gattcactgt ggcttgagaa aaaataaaga
     2761 aaaaagagat ttagctttgg tttattcaga tgttttatgt gatgcagcag cagtagtaac
     2821 tcaaaataaa gtaaagggca atcctgttta tgttacacag gaacacatta aaaatggaaa
     2881 agcacaagct attatagtaa atagtgctaa tgctaatacc tttaatggaa aagaagggct
     2941 tataaatgct tataaaatgg ctaaattcac cagtgataaa ttaaatatta aagaaagtga
     3001 tgttttggtt gcatctacag gtgttatagg aaagccatta aatatagatt taatagaaga
     3061 aaatatggat gaattagtga ataatctaag taaacaaggt catattggag caagagaagc
     3121 tattatgaca actgatatta taaaaaaaga aatagcagta gcaataatgt atggagataa
     3181 aaaaataact attggaggta tggcaaaagg ttctggcatg atacatccta atatggcaac
     3241 aacgttagga tttataacta cagatgcaaa tattgatgga gttttgctaa aagaagcttt
     3301 gaaaattgct gttgataaaa gttttaatag agttagtgta gatggagata caagtactaa
     3361 tgatatggtg atgattctag caaatggaaa agcaaaaaat gatagaataa acaaaaaaga
     3421 tgaacattat caagtatttt tgagtgcact tacatatgtc tgtatagaac ttgctaaact
     3481 tgttgcaaaa gatggagaag gatgtacaaa actaattgaa tgctatataa atggagcatt
     3541 aagtgaagaa catgcagtaa aattagcaaa aacagttata tcttcttcac tagtaaagac
     3601 agctgtattt ggagctgatg caaattgggg cagaatattg tgtgctttag gttatgcagg
     3661 tgaagaaata gatatggaaa aagttgatat tatttttgag agtatgaaag gatatattga
     3721 agtatgtaaa aatggaaatg gattagattt taatgaggaa aaagcaaaaa aaatccttga
     3781 agatgacgaa ataagtatat tagtagattt aaatatgggc aatgccagag gaaatgcatg
     3841 gggatgtgat ttaagctatg actatgtaag aataaatgga agttatagaa attaggagga
     3901 tatactttaa tgattaatat agaaaaagca aatacactta tagaagcttt accatatata
     3961 gaaaaacatc agggaaagac tattgtagtt aagtatggtg gaagtgcaat gaaaaaagat
     4021 ggtctaaaag aatctgttat ggaagacctt gtgttaatga gttatgttgg aatcaatata
     4081 gttttagtac atggtggagg ggcagaaatc aataagatgc tagcaaaagt ggatatagaa
     4141 agtaaatttg taaatggtct tagatataca gatgaagaga ccatggaaat tgtaaaaatg
     4201 gtgcttgctg gaaaggttaa taaagaccta gtaaataaaa tacatacaaa aggtggaaaa
     4261 gcagtagggc tttgtggaat agataataat atgattttat gtgacccata taaaaattat
     4321 gaacttggat ttgtaggaga aatcaaaaaa gtaaatgttg agcttataga atcttgtcta
     4381 aagtcaggtt atatttcagt aattgcaact ataggtgttg gagatgatgg agagacttat
     4441 aatataaatg gtgacacagc agcttctgct atagctaaag agttaaatgc agataaattg
     4501 atacttctta cagatgtccc tggtttgtta agagagccag atgaagaaaa atcattaata
     4561 acagaagtta tattagagga tgtcgataaa ttatttgaag aaggtataat tactggaggt
     4621 atgataccta aaatacaggg atgcgtagat gccttaaata acggagtaaa tagggtacat
     4681 attttagatg gaagagttcc acattctata ataacagagt tatttactga tagtggaata
     4741 ggtacattaa taagaaaaga aaatgaataa aattataagt ttaagaaggg agaataagaa
     4801 tgaataacaa caatacaatt tcaaaatgga acgaatattt tatagataca tataatcaac
     4861 ctaattttgt catagattat ggtgaaggaa gttgtttttt tgatactaat ggcaataaat
     4921 acatagattt tacaagtgga tatggagtat cttctcttgg gtatagtaac ggtaatttaa
     4981 agaatgctct aaaagagcaa gtagataaat tattacatac ttcaaattta tattttaatg
     5041 agccagtgtt gtattcagga gagaaaataa ttaattcaag tggaatggct aaagtgtatt
     5101 tttgtaattc tggaacagaa gcaaatgaaa cagcattcaa aatagcaaga aagtatagta
     5161 gtgataaata tggtaatggt agaggaacta tcataagttt aaaagactct tttcatggta
     5221 gaacaatgat gtctttaatg gcaactggta tggacaaata ccataagtac ttttatccac
     5281 ttcccgaagg ttttaaatat atagaaagaa ataatattga agatttaaaa aataatattg
     5341 attcgacagt ttgtgcaata attcttgaag cgattcaagg cgaaggtgga gttaatgttc
     5401 ttgaaaaaga ttatgttttg gaactagtga aaatatgtca agaaaaggat attgtagtga
     5461 tttttgatga agtccaatgc ggtataggaa gaactgggaa actttttggc tatgaatatt
     5521 ttgatgtaaa accagatata gttaccgtag caaaagggtt gggagcagga ataccagtag
     5581 gaggagtatt ggtaaataaa aaactttcaa aagtactagg aaaaggagac caaggtacaa
     5641 cttttggagg taatttatta gctatggtgg caacttcagt tgtattagat gagatatcta
     5701 aagatggttt ttataatgaa gtattagaaa aaggaaatta tataagaaaa tctattgaaa
     5761 gttttaataa taaagttgta ttaaagacaa aaggtattgg tctaatgata ggaattgaaa
     5821 ctaatattga atctagtatt atagaagaga aagctagaaa aaaaggattg cttatcttga
     5881 ctgctggaaa aaatgtattg agatttttac caccattaac aatcagttat aaggaaatag
     5941 atgaggctct tgaaatccta aaagatatat tactagaaac taactaaata ttcgaaaaat
     6001 agtttaatta aagacaaaaa ttagaagtaa ataaaaaagt aaagtgttaa caagtttaat
     6061 tgaatttaaa aagtttataa aaaggggcga attaatatga ataatataag tttaaaaggg
     6121 aagagttttt tgacattaaa ggattttaca aaagaagaga ttagatattt attaacttta
     6181 tcaagttatt taaaaactaa gaaaaaagtt ggtataaaag gcgatttgct acaaggaaaa
     6241 aatatagctt tattatttga gaaaacatca acaagaacta gatgttcatt tgaagtagca
     6301 gctcatgatg aaggagcaca tgtaacttat ctaggtccaa atgattgtca tatgggtaaa
     6361 aaagagtctg tatcagatac agcaaaagtt ctaggaagat tctatgatgg aatagaattt
     6421 agaggcttta aacaagaaac agttgaaagt ctagcaaaat attctggtgt acctgtttgg
     6481 aatggactta cagatgaata tcatccaaca caaatactgg cagacttttt aacggtaatt
     6541 gaaaatgtag ataaagattt gaataaagtt aaatttgtat atgttggtga tgctagaaat
     6601 aatatgggaa actcactgat gataggatgt gctaaaatgg gaatccattt cgtagcacta
     6661 gcacctaagg aactttggcc aaatgaagaa ttggttaagg aaatgaagga aatagccaag
     6721 aaatccaaag gagatataat gttgactgaa aatattgatg atgttaaagg tgcagatgtt
     6781 atatatacag atgtatgggt ttctatggga gaagaggagc aatatgaagc aagaataaat
     6841 caacttaaag attatcaagt aaatatggat atgataaaga aaactgaaaa tgaaaatgtt
     6901 atatttttac actgtcttcc agcatttcat gatttagaaa ctaaggttgc tcaggagatt
     6961 tatgaaaagt tcggattatc agaattagag gttacaaacg aggtatttaa ttcaaaatat
     7021 tcaaaggtat ttgaagaagc tgaaaataga atgcatacaa taaaagctgt tatggtttct
     7081 actatgactg atgtaaatat gaacaataat ttagtataga tatgttttaa tatagacttt
     7141 actttaatga aaatttataa aatattttaa atatattatt caaaaactct cattatatgt
     7201 taaaataact tatgtatcaa caaattaaga gggaggattt gagatatgaa aaaaatcata
     7261 ttagctttaa caactttttt attagcaata tctttaacag catgttcaag tgcaaaacca
     7321 gaagatacaa ttgatagttt ttttaacagt gcgaaaaagt ttgactttga aggcatgaat
     7381 aaggtaatgg aaaataatga cgaaaaatat aaggatatat taaaggaatt agatacaaaa
     7441 gacccaaatg cccaatatgt tttagattat ctaaaacaga atgcatctaa aatcacatat
     7501 acaataaaag acagcgaggt taaaggtgat aaggctacaa tcaaagtaga atgtaaattt
     7561 atagattcaa caccattact tcaagaaata gtagctgagg catttacaaa gatgattggt
     7621 atgagttttt caggacaaga cttaactgat gagaaaacaa ctgaaatgtt agtatcaatt
     7681 atgaaagaaa aacaaaaaag cattaaagag acttatgtta caaaaaatgt agagtttgaa
     7741 tgttctaaaa aggataataa atggataata agttcagtaa atgatgcagt agctgatgta
     7801 ttgttatcta atcttgttac agcaggacaa gaattttcta actctatgaa tacacttgaa
     7861 taatattgtt taggtgtagt aaagtaataa atatatgatt atttttataa ataaacatat
     7921 aaatatttat gaaggatacc atttttggta tcctttttat taatataatg aaatacatgg
     7981 ttaatttcta
//
LOCUS       DBGJFF010000155         7938 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.158, whole genome shotgun sequence.
ACCESSION   DBGJFF010000155 DBGJFF010000000
VERSION     DBGJFF010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7938
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.158"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            <1..561
                     /locus_tag="ACOIAF_003388"
     CDS             <1..561
                     /locus_tag="ACOIAF_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HGZ2670381.1"
                     /translation="GGIIGINIYINMNKLFPSDINRFKAIIGTSLVLGVIGLGVISKN
                     YHNLLTFKFRPDRKISKILIEENREIIKDINKDTVDIVGTFESFKKGIITIKAGSNNK
                     VKRPENLIDSDGNIRIYLNENTKLVSYIITKESKMDIVKYEEFDVKNLDLLRRYDTIN
                     VWIDKENQPKDKHGIMASKLLIGLYE"
     gene            676..1461
                     /locus_tag="ACOIAF_003389"
     CDS             676..1461
                     /locus_tag="ACOIAF_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HGZ2670382.1"
                     /translation="MEIIKYILYFFIYSFLGWTVESIGCSIASKRIINRGFLNGPICP
                     VYGFGAVIVISLLGRFNNVVIVFLLGMILTTILEYFTGFILETLFHAKWWDYSDRKFN
                     IKGRVCLKNAIYFGVMSVLIIRFIHPFIKYFVSIIPYRILISMAIITTLWTILDLIVT
                     IITLKKLDIKLNLLDDIITDLNDINVKLDEFDRGEIQALFKTINNDELEVREKINKIN
                     SKLDRIESNIILQKRVIKAFPHIKHKKQQEQLEHFKKIINEKR"
     gene            1753..3072
                     /locus_tag="ACOIAF_003390"
     CDS             1753..3072
                     /locus_tag="ACOIAF_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HGZ2670383.1"
                     /translation="MSEITSQMISTEEKKYVAKTQKIPYYPVAFKSGDGAMLYDYEGN
                     EYVDFLASAGSANVGHGNKEISQAVKEQMDDITQYTLAYFHSDPPVKLAEKLVEIAPG
                     DNDKKVLYSATGSACIDAAIKLARGYTGRTKIISMCESYHGSTYGAISISALSTNMRR
                     KMGPLLPEVYHFHYPDKNRTAKECLDEIEYAFAHYLPAEEVAAIFIEPIAGDAGIIVP
                     PVEWVQGLSKICKENGILLVSDEIQQGMGRTGKWFGIENFGVEADLIVLGKSVGGGLP
                     LGAVVGRTEIMQSLDAPAHLFTLAGNTTVCVAALKSIEIIEKENLLQKSIEMGDYIKA
                     GFEKLKEKYDIIGEIRGIGLSIGVDIVKGKGSNEKHPDATAKICYRCIQTGLIMIFLG
                     QSTLRVQPPLVITKEQVDKAMNIIDSAIDDYLNGRIGDEVYEVTQGW"
     gene            complement(3147..3575)
                     /locus_tag="ACOIAF_003391"
     CDS             complement(3147..3575)
                     /locus_tag="ACOIAF_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670384.1"
                     /translation="MKDNNNTNKTIQFGEKKASLENNTQKNKNIKLDNTHKVIPYPKN
                     RKKDDKKKGNKKKNTNTSKVNKNNYGHSFFNNKYKYKRKPYVIALYTVILILLAILII
                     TKLTDSTNKGNYSRNTVSDTHKNDIRNIVFNESNNFLICG"
     gene            complement(3666..5042)
                     /gene="hydG"
                     /locus_tag="ACOIAF_003392"
     CDS             complement(3666..5042)
                     /gene="hydG"
                     /locus_tag="ACOIAF_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HGZ2670385.1"
                     /translation="MFINHELINSLLEDAKNSTSDDIEKVLDKADRREKLSYKDIATL
                     LEVEDKKQLDRLFSIAGQIKNEIYGNRVVLFAPLYVSNYCVNECVYCGFSKCNKFKRK
                     KLTMEEIKEEVKILEKMGHKRLALEAGEDPKNCDINYILDCLDAIYSTYNENGNIRRV
                     NVNIAATSVDEYKLLKEKGIGTYILFQETYHKPTFIKMHGQSIKNDYYYHLTAFDRAM
                     EAGVDDVGAGVLFGLSDPKFEVLGLMMHNEHLEEKFGVGFHTISFPRLKKAEGMSLED
                     FPHLVSDDMFKKIVAITRLAVPFTGIIMSTRETAEMRNELLKYGVSQISAGSLTGVGG
                     YKAYEDGDNTEQFEVGDHRSPVEVLKELITDGYIPSYCTACYRKGRTGDRFMSLAKSR
                     QIHNVCTPNALTTLNEFLIDYGDEELKIMGKKLIAEEIGKIEREDIRNIVSNNMTALE
                     RGERDLYL"
     gene            complement(5043..6128)
                     /gene="hydE"
                     /locus_tag="ACOIAF_003393"
     CDS             complement(5043..6128)
                     /gene="hydE"
                     /locus_tag="ACOIAF_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HGZ2670386.1"
                     /translation="MTKNKIKYFIDKLYSINSLSFDELLYLIENIDNEEYNFEQNLRN
                     YLYKQASDMRNKHYGNRVYLRGLIELTNYCKNNCYYCGIQSSNKNIIRYRLSLDEILE
                     CCDIGYKIGYRTFVLQGGEDAYFTDDKICEIVSSIKNKYEDCAITLSLGEKSYDSYKK
                     YFLCGADRYLLRHETATDSHYKKLHPPNIELRTRKECLKNLKEIGYQVGAGFMVESPF
                     QNNEDLVRDLLYLKELNPHMVGIGPFIPHHDTIFKDYKHGDLEKTLLMLSITRLLLPK
                     VLLPATTALASINPSGRNAGLLAGCNVIMPNLSPQEFRKQYSLYDNKAFTGQEACEYH
                     QSLEENIKSLGLEVDYSRGDNIEWRRL"
     gene            complement(6164..7372)
                     /gene="hydF"
                     /locus_tag="ACOIAF_003394"
     CDS             complement(6164..7372)
                     /gene="hydF"
                     /locus_tag="ACOIAF_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HGZ2670387.1"
                     /translation="MSLNSTPQSVRVHIGLFGKRNAGKSSIINAITNQSAAIVSDIAG
                     TTTDPVFRPMEILPIGPCVLIDTAGLDDVGELGELRIGKSLDVLEKTDIALLVVDCQI
                     GISQEDLSLIEKFNDKNIPHILILNKIDTIKNQSEILNLTKNKVKCPVVSVSSTDKIG
                     IENLKNEIIKVLPKDSTEFKLVSDLIEPNDLVVLVVPIDKAAPKGRLILPQQQVIRDI
                     LDNGAISIVTKEDSLKETLSNLGKKPKLVITDSQVFPQVDKDTPKDIPLTSFSILFAR
                     QKGDLKELINGAYALENLKDGDKILMAEGCTHHRQTDDIGTVKIPNMIRKKTGKNITF
                     EFSSGVSFTEDINKYALVVHCGACMMNRAGMLSRIEKAKSFNVPIVNYGVLIAYVKGI
                     LERSLELFNY"
     gene            complement(7387..7638)
                     /locus_tag="ACOIAF_003395"
     CDS             complement(7387..7638)
                     /locus_tag="ACOIAF_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0065007 - biological regulation [Evidence
                     IEA];
                     GO_process: GO:0065009 - regulation of molecular function
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TM1266 family iron-only hydrogenase system
                     putative regulator"
                     /protein_id="HGZ2670388.1"
                     /translation="MKKVAVISAILEEPKECQFKFNEVVSGFKGIIKGRMGIPCEEEG
                     VSIVCIAVVGEMDTINSLTGKLGNIPHVSVKTSISKKEV"
BASE COUNT         2828 a         1101 c         1163 g         2846 t
ORIGIN      
        1 ggtggcataa ttggaattaa tatttacata aacatgaata aattatttcc cagtgatatt
       61 aacagattta aggctataat aggtactagt ttagttttag gagttatagg gttaggagtt
      121 atttctaaaa attatcataa cttactaact tttaaattta gacctgatag aaaaatatca
      181 aaaatattaa ttgaagaaaa tagggaaata attaaagata taaataaaga tacagtagat
      241 atagttggga catttgagag ctttaaaaaa ggaataataa ctattaaagc aggttctaat
      301 aacaaagtaa agagaccaga gaatttaatt gattctgatg gaaatataag aatttattta
      361 aatgaaaata caaaattagt atcttatata attaccaaag aatcaaaaat ggatatagtt
      421 aaatatgagg aatttgatgt gaagaattta gatttattaa ggagatatga tactattaat
      481 gtttggattg ataaagaaaa tcagccaaag gataaacatg gtattatggc tagtaaatta
      541 ttaattggat tgtatgaata aaatattaaa caagaataaa agaataacaa atataaaaaa
      601 cgaaaaaact atttctataa ttatttttac gttgtgcata aaataagaac attagtgaag
      661 gagtagagat aaattatgga gataataaaa tatatcttgt acttttttat atattcattt
      721 ttaggatgga cagtagaaag tataggatgt tctatagcaa gcaaaagaat aatcaacaga
      781 ggttttttaa atggtccaat atgccctgtt tatggttttg gagcggtaat tgtgatatca
      841 ctattgggaa gatttaataa tgtagtaata gtatttctat taggaatgat tttaactacg
      901 atactagaat attttacagg atttatttta gaaaccttat ttcatgctaa atggtgggat
      961 tattctgaca gaaaattcaa tataaagggt agagtatgtt taaaaaatgc aatatacttt
     1021 ggtgtaatgt ctgttttaat aataagattt atacatccat ttataaaata ttttgtaagt
     1081 ataattccat atagaatatt aatttcaatg gctataatta caacactttg gactatactt
     1141 gatttgattg ttactattat aacacttaaa aaattagata taaagttaaa cttattagac
     1201 gatataatta cagaccttaa tgatataaat gtgaaattgg atgaatttga tagaggagaa
     1261 atacaagctt tatttaaaac aataaataac gacgaattag aagttagaga aaaaataaac
     1321 aaaataaata gtaagttgga ccgtatagag tctaatatta ttttacaaaa aagagttata
     1381 aaagcttttc cacatataaa acataaaaaa caacaagagc aactagaaca ttttaaaaaa
     1441 attattaatg agaaaaggta attatacaat ttaatgaaga cgaatagaat aatggtattt
     1501 ataagatgat tttcagagga ctctataaaa atagaataga gtcctttatt ttttgaaaaa
     1561 taatgtttat ttgataaaaa taaaatatat tgtttctgaa taacaaatat tttgaaaatt
     1621 ataaaaacat atattgacaa tttgtgtcaa aaatactaac attaagtata taaagaattt
     1681 tatatgagaa tatctttata aatcgcaata attttaaaac ataaatttat ttaaagaggg
     1741 gatgatttgc caatgagcga gataactagt caaatgataa gtacagaaga aaaaaaatat
     1801 gttgctaaaa ctcaaaagat accttattat ccagtagcat ttaaatcagg tgatggtgca
     1861 atgctatatg attatgaagg taatgaatat gtagattttt tagcaagtgc aggctctgca
     1921 aatgtaggtc atggtaataa ggaaatctca caagcagtta aagaacaaat ggatgatata
     1981 acacaatata cacttgcata tttccatagt gatcctcctg taaaattagc tgagaagctt
     2041 gtggaaatag cccctggaga taatgataaa aaagtactat acagtgcaac tggttctgct
     2101 tgtatagatg ctgctataaa attagcaaga gggtatactg gaagaacaaa gataatatct
     2161 atgtgtgaat cttatcatgg aagtacttat ggagctatat ctatatctgc tttaagtaca
     2221 aatatgagaa gaaaaatggg tccattgtta ccagaagttt atcacttcca ttatcctgat
     2281 aaaaatagaa cagctaaaga atgtttagat gagatagaat atgcttttgc acactatcta
     2341 ccagcagaag aggtagctgc aatatttata gagccaattg caggagatgc aggaataata
     2401 gtgcctccag tagagtgggt acaaggttta agtaaaatat gtaaagaaaa tggaatatta
     2461 cttgtaagtg atgaaataca acaaggtatg ggaagaacag gtaagtggtt tggtatagaa
     2521 aattttggtg ttgaagcaga ccttatagtt cttggaaaat ctgttggagg tggattacct
     2581 ctaggagcag ttgttggaag aacagaaata atgcaaagtc ttgatgcacc agcacattta
     2641 tttactttag ctggaaacac aacagtatgt gtggctgctt taaaatctat agagataatt
     2701 gaaaaagaaa acttacttca aaagagtata gaaatgggag attatattaa agcaggattt
     2761 gaaaaactaa aagaaaaata cgatataatt ggagaaataa gaggcatagg attatctata
     2821 ggagttgata tagttaaagg aaaaggttct aatgaaaaac atccagatgc tacagctaag
     2881 atatgttata gatgtataca aactggatta ataatgatat tcttaggaca atctacttta
     2941 agagtgcaac cacctctagt aataactaag gagcaagtag ataaagcaat gaacatcata
     3001 gattctgcaa tagatgatta tttaaatggt agaattggtg atgaagtata tgaagtaact
     3061 caaggttggt aaaataagta ttaaatgaaa ataggagtta tctcaatttt agaaaattat
     3121 ttctgaaatg agataactct tttaagttac ccacaaataa gaaaattgtt tgattcatta
     3181 aagactatat ttcggatgtc gtttttgtga gtatcagaga cagtattacg agaataatta
     3241 cctttattcg tactatctgt taatttagtt ataattaaga tagctaaaag aataagaatt
     3301 actgtgtata aagcaataac atatggtttt cttttatatt tgtatttgtt attgaaaaaa
     3361 gaatggccat aattattttt atttacttta gatgtatttg tattcttttt cttgttacct
     3421 ttctttttat catccttttt tctattttta gggtaaggaa tgactttgtg tgtattatca
     3481 agtttgatat ttttattttt ttgagtattg ttttccaggc tagccttttt ttctccaaat
     3541 tgaatagttt tattggtatt gttgttatct ttcaatgtta atctccctcc cttaaacttt
     3601 ccctttagat aatattacta ctaacctatg gaatatacaa taaataatat ataagtaata
     3661 aaattttata ggtataaatc tctttctcca cgctctaagg cagtcatatt gttagatact
     3721 atgtttctta tatcttctct ttctatctta cctatttctt ctgcgataag ttttttaccc
     3781 ataattttaa gttcttcatc tccataatcg attaaaaatt catttaatgt agtaagagca
     3841 tttggtgtac atacattatg gatttgtcga cttttagcta agctcatgaa tctatcgcca
     3901 gttctacctt tacgataaca agctgtacaa taactaggta tatatccatc agtaatcagt
     3961 tctttaagca cctcaacagg gcttctatgg tctccaactt cgaattgttc tgtattgtca
     4021 ccatcttcat atgctttata tcctccaaca cctgttagag aacctgcact aatttgtgat
     4081 acaccatatt ttagtaactc atttctcatt tcagcagttt ctctagtgga cattatgatt
     4141 ccagtaaaag gaacagccaa tctagttata gctactattt ttttgaacat atcgtcagaa
     4201 actaagtgag gaaaatcttc taagctcata ccttcagctt ttttcaatct agggaaagat
     4261 atagtatgga aacctacacc aaatttttcc tctaagtgtt cgttgtgcat cataagtcct
     4321 agaacttcaa atttagggtc agataaacca aataaaactc ctgcaccaac atcatcaaca
     4381 ccagcttcca tagctctatc aaaagctgtt aaatggtaat aatagtcatt ttttatagat
     4441 tgaccatgca tttttataaa tgttggtttg tgataagttt cttggaataa tatataagtt
     4501 cctattcctt tttcttttag taatttatac tcatctacac ttgtagctgc tatgttaacg
     4561 tttacccttc ttatatttcc gttttcatta tatgttgaat aaattgcatc taagcaatct
     4621 aagatgtaat ttatatcaca atttttaggg tcttcaccag cttctagagc aagtctttta
     4681 tgacccattt tttctaatat tttaacctct tcctttatct cttccatggt aagttttttt
     4741 cttttaaatt tgttgcattt actaaaaccg caataaacac attcatttac acagtagttt
     4801 gatacataaa gaggggcaaa taaaaccact ctattaccat atatttcatt ttttatttgc
     4861 ccagctatac taaataatct atctagttgt tttttatctt ctacttcaag aagtgtagct
     4921 atatctttat aactaagttt ttcccttcta tcagccttat caagtacctt ttcaatatca
     4981 tcagaagttg aattttttgc atcttctaat aaagaattaa taagttcatg gtttatgaac
     5041 atttacaaac gcctccattc aatattatca ccgcgagaat agtcaacttc aagtcctaat
     5101 gacttgatgt tttcttctaa actttggtga tattcacaag cttcttgacc agtaaaggct
     5161 ttgttatcgt aaagagaata ttgttttcta aattcttgag gagaaaggtt tggcataatt
     5221 acattgcaac cagctaaaag acctgcattt ctaccactag ggtttataga tgccaaggct
     5281 gttgtagcgg gtaataaaac ttttggaagt agaagtcttg taattgaaag cataagtaat
     5341 gtcttctcta aatctccatg tttataatcc ttaaaaattg tatcatggtg tggaatgaaa
     5401 ggtcctattc caaccatatg aggatttaac tcttttaggt ataataaatc acgaacaagg
     5461 tcttcgttgt tctgaaatgg tgattcaacc ataaatccag cacctacctg ataaccaatt
     5521 tctttaaggt tttttaggca ttcttttcta gttctaagct caatgtttgg cggatgtaat
     5581 tttttataat gactgtctgt agcagtttca tgtcttaaaa gatatctatc tgcaccacat
     5641 aaaaagtatt ttttatacga atcataactc ttttctccta gtgatagagt tatggcacaa
     5701 tcctcatatt tattttttat acttgaaaca atttcacata ttttatcatc agtaaaatat
     5761 gcatcttcac caccttgcaa aacgaatgtt ctgtatccta ttttataacc tatatcacaa
     5821 cattcaagta tttcatcaag tgaaagcctg tatcttatga tatttttatt agaagattgt
     5881 attccacaat agtagcaatt atttttacaa tagtttgtaa gctctataag tcctctcaag
     5941 tagactctat ttccataatg tttatttctc atgtcgctag cttgtttata aagatagttt
     6001 cttaaatttt gttcgaagtt atattcctca ttgtcgatat tttctattaa atatagtagt
     6061 tcatcaaaac ttaatgaatt aatgctgtaa agtttatcta taaagtattt aatcttattt
     6121 ttagtcatat tcaaatatgt aattattaag tatagttaaa tttttaataa ttgaaaagtt
     6181 caagtgatct ttccaaaata ccttttacat atgctattaa gacaccatag tttacaatag
     6241 gtacattgaa agattttgcc ttttctattc tagataacat tccagctcta ttcatcatac
     6301 aagctccaca gtgaactaca agggcatatt tatttatatc ttcagtgaag gatactccag
     6361 aactaaattc aaaagtaatg tttttgccag ttttctttct aatcatatta ggaattttta
     6421 ctgttccaat atcatcagtt tgtctatgat gggtacatcc ttcggccatc aagattttat
     6481 caccatcttt taaattttct aaagcataag ctccattaat caattcttta aggtctccct
     6541 tttgtctagc aaacaatata gaaaaagatg taagaggaat atcttttggt gtatctttat
     6601 ctacttgtgg aaatacttga gagtcagtaa taacaagttt tggttttttg cctaaattag
     6661 atagagtttc ttttaaacta tcttcttttg taactataga aattgcacca ttatccaaaa
     6721 tatctcttat aacttgctgt tgaggaagta taagtctacc ttttggagct gctttatcta
     6781 ttggaacaac taaaacaact aaatcatttg gttcaattaa atctgatacc aatttgaact
     6841 cagtgctatc cttaggcaaa acttttatta tttcattttt taaattttca attccaattt
     6901 tatctgttga tgagacagaa acaactggac atttaacttt atttttagtt aagtttaaaa
     6961 tttctgattg attcttaata gtatcaattt tgttaagaat tagaatatga gggatattct
     7021 tatcattaaa tttttcaatt agtgacaaat cttcttgtga tataccaatt tggcaatcca
     7081 ctactagtaa agctatatct gtcttttcta aaacatctag tgatttacct attctaagtt
     7141 caccaagttc accaacatca tcaagaccag cagtatctat aagaacacaa ggtcctatag
     7201 gaaggatttc cattggtcta aatactgggt ctgtagtagt tccagcaata tctgagacta
     7261 ttgcagcact ttgatttgtt atagcattta ttatgctaga tttacctgca tttcttttac
     7321 caaagagacc tatatgtact cttaccgatt gtggagtaga atttagactc atttgtactc
     7381 accaccttaa acttcttttt ttgatatact agtttttaca gatacatgag gtatgttgcc
     7441 caatttacca gtcaaactat taatagtatc catttcgcca acaactgcta tacatacgat
     7501 tgagacacct tcttcttcgc aaggaatgcc cattcttcct ttaataatac ctttaaaacc
     7561 tgatacaacc tcattgaatt taaattgaca ttcttttggc tcttccaaaa tagcacttat
     7621 aactgcaacc tttttcataa atatcctcct aactcagaaa atatgtctta aacaaatcca
     7681 aaatcaaaaa acttttcttt gtaaagaaaa gtttctacaa aagaaaagtt attaattaca
     7741 caagtataat gtgctatatt aaactatttc cttacagatt agataactct tttctggtag
     7801 ggttagcttg taaaaattta atttaacaac aagtaactga agaaaatttt tatacaattt
     7861 atataaatta taataaaaat taactaaaga aaattgaagt tacttatata atagtagtaa
     7921 attgaaatta agtctata
//
LOCUS       DBGJFF010000156         7927 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.159, whole genome shotgun sequence.
ACCESSION   DBGJFF010000156 DBGJFF010000000
VERSION     DBGJFF010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7927
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.159"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(449..1267)
                     /locus_tag="ACOIAF_003396"
     CDS             complement(449..1267)
                     /locus_tag="ACOIAF_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HGZ2670389.1"
                     /translation="MFKIGDFSKLSKISIRMLRHYDEIGLLTPSHTNKTNGYRYYSAD
                     QLSTTNRIHALKDMGFGLYSIKEILTEYNDKESLIKYLNIHHSQVKEQLEDTQKKLLK
                     IETTIKRIGGNDIMKNYDVTIKNFAPKYMMTLRRVIPTYQDEGMLWHQAFLETKDQNV
                     QIEPPKYSKAVFYDTGYKEDYVDVEVQVAVSGKYKDTEHVKFKTVPSVTAATAIVNGN
                     FNQVADACEAIGNWISDNNYDVDGPMFNIYHVSPGNDSNPDNWVTEVCFPVKKK"
     gene            1435..2016
                     /locus_tag="ACOIAF_003397"
     CDS             1435..2016
                     /locus_tag="ACOIAF_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HGZ2670390.1"
                     /translation="MKSNDKDARERIIEVTLNLLNEVDDIEEITVRKIAERANVGVGL
                     INYHFKTKDNLLSTAIGDVMSNIIAELYDDSVYTLRPIEDLKNLLKKLCDTGLHYEKV
                     LPFVLNQCITNGDMQAELDIVPMLRKIFGNKKDEMSLRIIALQIILPIQISALSTESF
                     QLYSGINIKNKYERDKFIDILIENIIGEDVDVR"
     gene            2013..2207
                     /locus_tag="ACOIAF_003398"
     CDS             2013..2207
                     /locus_tag="ACOIAF_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670391.1"
                     /translation="MKIITIGSSLITVLLFLSTMVCGFWIKNNKVTDASSIKFHMNSA
                     IFTGIFLLISTILLIIYIKK"
     gene            complement(2371..4113)
                     /locus_tag="ACOIAF_003399"
     CDS             complement(2371..4113)
                     /locus_tag="ACOIAF_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M3"
                     /protein_id="HGZ2670392.1"
                     /translation="MVKSKKKIFVFLILIAVFIIFASYIRNYTNDTKKDSVRGTIPFE
                     KMEYKRPNIQSICENINKCNDKLLTAKTAKEQLNLFNEVDKIYQDFYSTLTIAKIRNN
                     IDSSDEFYSKEYKYLMSKSVDVDMSYKDFQDRFVTSKFSKELEKELGKDTFNYLKNIG
                     KLNSKEVEGLLKKEQDLVVKYEGLLAKSTVSIDGIEVDFEEALSRPNLSPEEYVKIYS
                     DYLKKYNPIFGNIFLELIQTRTEIASKQGFKNYIDYAYMNLNKDYSQEEAKKFRQDVK
                     DYIVPLYREISSKPSDSSIYIKVYKNRSFRKFDKVLEDISPKLKESFDYMKKYDLYDY
                     SSGKNKSPGGYTTYINKYKAPFLFNTWDNSFLGVTSFAHEFGHFYNYYNSINLNNKMQ
                     PSIDVCEVHSTSLEILFYKYFDDFFGKQSEAIKKEHLSIVLNTIIDACLYDEFQEIIY
                     KNPYMSLNDINKLFFDLEEEYGVSNNILREKNAPFWILVSHNFQVPFYYLSYGLASDV
                     SLQIWQLSQEDYRKAVDVYMDFLNQNTDAGFKDVVEKVNLQLPFQNGNLEEISSTLYD
                     YFGIDNPLELKNAS"
     gene            4542..5918
                     /locus_tag="ACOIAF_003400"
     CDS             4542..5918
                     /locus_tag="ACOIAF_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HGZ2670393.1"
                     /translation="MKTIVRRLILYADGMRPTQIMVSGFATIIVIGALLLTLPIASQS
                     GESIGLLNALFTATSAVCVTGLVMVDTATYWSLFGQIVIITLIQIGGLGFMTVATMFS
                     LMARKKIQLRERLLIQESLNQADLSGLVRLTRFVLIITITIEGIGALVLSTVFIPQFG
                     LSKGIWYSVFHAISAFCNAGFDLMGSVSGPFTSLNSYVNNFTVSMTVCALIVLGGLGF
                     PVVLDIVRKRRFSKLNVHSKVVLFSTATLIFVGALFIFLIEFNQKATMANLPLKGKVL
                     SAIFQSVTARTAGFNTLDLATLRESSVFVMIILMFIGASPASTGGGIKTTTLAVLIIT
                     VRSFLSGKSDIEAFERRLAPSTIKKSLGIFVISISAVIFGTLIISITQPNFTLVQSAF
                     EVTSALATVGSSLAGTPNLNALGKIIIIIFMFMGRVGSLTLFMAILSGGRRKSQPIRY
                     AEGKIMVG"
     gene            6229..6900
                     /locus_tag="ACOIAF_003401"
     CDS             6229..6900
                     /locus_tag="ACOIAF_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HGZ2670394.1"
                     /translation="MKQYIVIGCGRFGSSVASTMHLLGHQVMAIDKNEDSVQCISDKV
                     THSLIVDVTDEQALRSLGLGNFDVAVVAIGSDIRASIMATLIAKEMGVELIICKAKDE
                     LQAKVLYKIGADRVVFPERDMGVRVAHNLVSDNILDHIELDPEYSIVEIVTPNSWVGK
                     TLIELELRARYEITVLAIKTGKNINVTPSPDEELTAGSILVIIGQNTSITAITSGNKG
                     IIRRR"
     gene            7089..7886
                     /locus_tag="ACOIAF_003402"
     CDS             7089..7886
                     /locus_tag="ACOIAF_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HGZ2670395.1"
                     /translation="MITNINSKDNEKLKYTRALLKSKNRNKESKFIIEGYRIVMLALE
                     CMANLDYVFINEEFEHKKEHVKLLEDLDKKNTKIYKTTNKNFKELVDTENTQGIIGVV
                     SFKKKKLSESINKKDKFVLILDRIQDPGNMGTIIRTADSAGVDAIIALKGCVDIYNPK
                     VIRSTMGSIFDMNIIDASQDETVDMLKSLDFNIVSSYLNTENFYDKIDYGSKVALVIG
                     NEANGINEELVSKSDILVKIPIYGKAESLNAAISSAILMYEIKKYLI"
BASE COUNT         2927 a          973 c         1219 g         2808 t
ORIGIN      
        1 attgatttat ctgacaaatt gagataatat cctaattaaa ttgtaggtat ttaaacataa
       61 cattataaat aatttatctg acaaattaag ataatatcct aatcaaattc tagtcattta
      121 aacacaacat cacaagtgat ttatctgaca aattaagata atatcctaat taaattgtag
      181 gtatttaaac ataacatcac aagtgattta tctgtcaaat taagataata tcctaattaa
      241 attctagtca ttcaaacata acatcacaag tgatttatct gacaaattaa gataatatcc
      301 taattaaatt gtagatattt atacataaca ctaaaagtaa tttgtttaac aaattaagat
      361 aatatcctaa ttaaattgta ggtatttata cataacacta aaagtaattt gtctaacaaa
      421 ttaaaatatt atccaattaa tataaaaatt acttcttttt aactgggaag catacctctg
      481 ttacccaatt atcaggatta ctatcgttgc caggacttac atgataaata ttaaacatag
      541 gaccatccac atcatagtta ttatctgata tccaattacc aatagcctca caagcgtctg
      601 caacttgatt gaaatttcca ttaacaattg cagttgcagc agtcacactt ggaacagtct
      661 taaatttaac atgttcagta tctttatact ttccagatac agcaacttgt acttcaacat
      721 ctacataatc ttctttatat ccagtatcat aaaaaactgc tttagaatac tttgggggtt
      781 caatttgaac attctggtct tttgtctcta aaaatgcttg atgccataac attccttcgt
      841 cttgataagt tggtataacc cttctaagtg tcatcatata ttttggtgca aaattcttta
      901 tagttacatc ataatttttc ataatatcat ttcctccaat tcttttaata gtggtttcta
      961 tttttaatag ttttttctgt gtatcttcta attgttcttt tacttgagaa tggtggatat
     1021 ttagatattt tatgagactt tctttatcgt tatattctgt caaaatttct tttatagaat
     1081 acaagccaaa gcccatatct tttagtgcat ggattctgtt tgtagtagaa agttggtctg
     1141 cactatagta tctatatcca ttagttttat tcgtatgaga aggagttaaa agtcctattt
     1201 catcataatg acgtaacata cgaatactaa ttttacttag ctttgaaaaa tcgcctatct
     1261 taaacatgtt tttgtctcct ttataaaggt acttttagct taatattaat ataaaccata
     1321 tcataatgtt agagtcaact aaaattataa ttaaattata ttgacatatg atgaaaaaaa
     1381 tattatactg aacatgttca gaaccgaaaa tgttaattgt tttggaggtc tattatgaaa
     1441 agtaatgata aagatgctag agaacgtata attgaagtta cattgaatct tttaaatgaa
     1501 gtagatgata ttgaggagat tacagttaga aaaattgctg agcgtgcaaa tgttggagtt
     1561 ggtttaataa attatcattt taaaacaaag gataatttgt tgagtactgc cataggagat
     1621 gtgatgtcaa atataatagc agagctatat gacgatagtg tatatactct aagacctatt
     1681 gaagatttga aaaatttatt aaaaaaattg tgtgatactg gattacatta tgaaaaagtg
     1741 cttccttttg tgttgaatca atgtataacc aatggagata tgcaagcaga acttgatatt
     1801 gtccctatgc ttcgtaaaat ttttggaaat aaaaaagatg aaatgagtct aagaatcatt
     1861 gccctgcaaa ttattttacc aatacagatt tctgctttat ctacagaatc atttcagtta
     1921 tacagtggta taaatataaa gaataaatat gagcgagata aattcattga tattttaata
     1981 gaaaatatta ttggagagga tgttgatgta agatgaaaat tataactatt ggttcaagtc
     2041 ttatcactgt tttacttttt ctttcaacta tggtttgtgg gttctggatt aagaataata
     2101 aggtaacaga tgcaagttct attaagttcc atatgaatag tgctattttt acagggatat
     2161 ttttattgat ttccacaatc cttttaatta tatatataaa aaaataataa tattattaat
     2221 aaataaagta tcagaaatta taaagacaag agtcttaaaa atttctgata ctttaccata
     2281 taattttatt aaacattaat tgtgtgtgaa aaaatatcac ttatttcaat ctaacactaa
     2341 atcttaagag tgagtataaa gtattagaaa ttaagaagca ttttttagtt caagtggatt
     2401 atctatacca aaataatcat ataaagtaga gcttatttcc tctaaatttc cattttggaa
     2461 aggtaattgt aaatttacct tttctaccac atctttaaat ccagcatcag tattttgatt
     2521 taaaaaatcc atatatacat caacagcttt tctgtaatct tcttgtgata attgccatat
     2581 ctgaagtgat acatcagatg caagaccata actcaagtaa taaaaaggaa cttggaagtt
     2641 atgagataca agtatccaaa aaggagcatt tttttctcta agtatattat tggaaactcc
     2701 atattcttcc tctaaatcaa aaaacaattt attgatatca tttagactca tatatggatt
     2761 cttgtatata atttcttgaa attcatcata aagacaagca tcaataatag tatttaaaac
     2821 aatcgataag tgttcttttt ttatagcttc agattgttta ccaaaaaaat catcaaaata
     2881 tttataaaat agaatttcta aagatgttga atgaacttca catacatcta tacttggttg
     2941 catcttattg tttaagttaa tactgttgta gtaattataa aaatgaccaa actcatgagc
     3001 aaaactagta acacctaaaa aagaattgtc ccatgtatta aacaagaaag gtgctttata
     3061 tttatttata taagtagtat atcctccagg tgatttgttt tttccagaag aataatcgta
     3121 caagtcatat tttttcatat agtcgaagct ctcttttagt tttggagata tatcttcaag
     3181 tactttatca aacttcctaa aagagcgatt tttatatact ttaatataaa tagaagaatc
     3241 agatggttta gatgaaatct ctctatataa tggaactatg taatctttta catcttgtct
     3301 aaatttttta gcttcttctt gagaatagtc tttgtttagg ttcatatagg cataatctat
     3361 ataattctta aatccttgct tacttgctat ttcagttctt gtctgtataa gttctaagaa
     3421 tatgttacca aatataggat tgtatttttt taagtaatct gaataaattt tcacatattc
     3481 ttcagggcta agatttggtc ttgacaaggc ttcttcaaag tctacttcta taccatctat
     3541 tgatactgta gatttagcta ataagccttc atattttaca actaggtctt gctctttttt
     3601 aagcaaacct tcaacttctt tagaattaag ttttccaatg ttctttagat aattaaatgt
     3661 atcttttcca agttcttttt ctaattcttt agagaattta gaagttacaa atctatcttg
     3721 aaagtctttg tatgacatgt caacatctac tgatttactc ataagatact tatattcctt
     3781 agagtaaaat tcgtctgatg aatctatgtt atttctaatt tttgctatag taagtgtact
     3841 gtaaaaatcc tgataaattt tatcaacttc attaaataaa tttaattgtt ccttagctgt
     3901 tttagctgtt aaaagtttgt cgttacactt gttaatgttt tcgcaaattg actgtatatt
     3961 aggacgctta tattccattt tttcaaaagg aatagttcct ctaacagaat ctttttttgt
     4021 atcattagta taatttctta tgtatgatgc aaatataata aaaactgcaa ttaaaattag
     4081 aaaaacaaat attttcttct tggatttaac cataaaaatc ctccaatcat ggatatagtt
     4141 attaattatt ttactatatt aaatataaaa aatgcatgtt aagaaaattt aacataaaga
     4201 tatagataag attataaata atataaaaaa tttaacttca aaaaagtata tgaaatttat
     4261 actggtaaaa tgataaataa attttaagta caaaatagta aaagctatga tagttttaga
     4321 cccattatat gtaaaaaata tagatgtttt tggtgcaatt taaattaaaa gatatatata
     4381 aaaatttaaa atattccaaa cagaaattat aaaaattact atcattgtct ttatacaata
     4441 tttatactat aatagaataa taatttagag gaaagctcta aaaataaaca aattaagggt
     4501 cttaacagta gtgattttat gttaaggagg tataaagaaa gttgaaaaca atagttagaa
     4561 gacttattct ctacgcagat ggaatgcgtc ctactcaaat aatggtatct gggtttgcta
     4621 cgattattgt aatcggagca ttgttgttaa cactcccgat agcgtcgcag agtggagaga
     4681 gtataggctt attaaatgct ctatttacgg ctacttcagc ggtttgtgtt actggtctag
     4741 ttatggtaga tacagcaact tattggagtt tatttggaca gatagttata ataacattga
     4801 tacaaattgg tggtcttgga tttatgaccg tagccacaat gttttcactt atggcacgaa
     4861 aaaaaataca actaagagaa agattattaa tacaagagtc attaaatcaa gctgatttat
     4921 cggggcttgt aagacttaca agatttgtat taataataac cataacaata gagggaatag
     4981 gtgcactggt tttatcaact gtctttattc cacaatttgg tttgtctaaa gggatatggt
     5041 atagtgtatt tcacgcaata tcagcgtttt gtaatgccgg atttgacttg atgggtagtg
     5101 taagtggccc atttacatcg ttaaattctt atgtaaacaa ctttacagta tcaatgactg
     5161 tttgcgcact tatagtgctt ggagggctag gattccctgt tgtacttgat attgtaagaa
     5221 aaagaagatt ttcaaaatta aatgtgcatt ctaaggttgt attgttttca actgcgacac
     5281 ttatttttgt gggtgcatta tttatttttc ttatagaatt taatcaaaaa gctactatgg
     5341 cgaacttgcc tttaaaggga aaagtactat cagcaatatt tcaatcagta acagcaagaa
     5401 ctgctggttt taatacgctt gatttagcta ctttacgaga aagtagtgta tttgtaatga
     5461 taattttgat gtttattgga gcatcacctg catcaactgg tggaggtata aaaacaacaa
     5521 cactagctgt tttaattatt acagttagaa gttttctatc aggaaaatca gatatagaag
     5581 cttttgaaag aagattagct ccatcaacta taaaaaaatc tcttggtata tttgtaatca
     5641 gtatatcagc agttattttt ggaacattga ttatttcaat aactcagcct aattttacat
     5701 tagttcaatc agcttttgag gttacatcag cacttgctac agttggttca agtttagctg
     5761 gaactccaaa cttaaatgct ttaggtaaga ttataataat aatctttatg tttatgggta
     5821 gagttggttc tcttacttta tttatggcta tactttctgg tggtagaaga aagagtcaac
     5881 caatcagata tgcagaaggt aaaattatgg ttggttaagt taccaagaag tagtataaat
     5941 ttataatgga tttagagtag cactgtaaat ctgttgataa aataaatgag cctagatgta
     6001 attatatgaa gaaataaaat caactaacga acaaaattaa ttaatgaaat aaaaaaacag
     6061 tgaaataaaa tacaaataaa aagggaaatc aaaattaaat taatctatgt atataaaatt
     6121 atatattgta tataagtttt gtatgtgtaa agtatataag ttttgtatgt gtaaataatt
     6181 aatttaagag atattatata aattgtgtat tagggaggaa gtttcgagat gaagcaatat
     6241 atagtcattg ggtgtgggag atttggaagt tcagttgcgt ctactatgca tcttttagga
     6301 catcaagtaa tggcaataga caaaaatgaa gattcagttc aatgtatatc tgataaggta
     6361 acccattcac ttatagtgga tgttactgat gagcaagcgt taaggtcatt aggtttaggt
     6421 aactttgatg tagcagtagt tgcaataggt tctgatataa gggcatctat aatggcgact
     6481 cttatagcca aagaaatggg tgtagagttg ataatatgta aggcaaagga tgaattacaa
     6541 gctaaagtgc tttataaaat tggcgcagat agagttgtat ttccagaaag agatatggga
     6601 gtaagagttg cacacaattt agtttcggat aatatattag accatattga acttgaccca
     6661 gagtattcaa ttgttgaaat cgtaactcca aatagttggg ttggcaagac acttatagag
     6721 cttgaattaa gagctagata tgagataact gtacttgcta taaaaacagg taaaaatata
     6781 aatgttacac cttctccaga tgaggaactt acagccggaa gtatcctagt tataatcggt
     6841 caaaatacta gtataacagc gataacatct ggaaataagg ggataattag aagaagataa
     6901 tttactattt aatatatatt taattgcaat gaaagtagat agtatcatat aattatgaat
     6961 agttatatga tactattttt ttaatcgaag gtagtattta tttgtaagat tagataaaga
     7021 gaagttaaat taaaagtaag gaggctgtgc taatataaaa atttataagt tattagcatt
     7081 tagataaaat gattacaaat ataaacagta aggataatga aaagttaaag tatacaagag
     7141 cactattaaa atcaaagaat aggaataaag agtcaaagtt cataatagaa ggatacagaa
     7201 tagtaatgct tgcacttgaa tgtatggcaa accttgatta tgtatttatc aatgaagaat
     7261 ttgaacataa gaaagaacat gtaaaactat tagaagattt ggataaaaaa aacacaaaga
     7321 tatacaagac tactaataaa aactttaaag aattagtgga tacagaaaat actcaaggaa
     7381 ttataggtgt agtttcattt aagaaaaaaa aattaagtga aagtataaat aaaaaagata
     7441 aatttgtatt gattttagac agaatacaag acccaggaaa tatggggact ataataagga
     7501 ctgctgattc tgctggagta gatgccataa tagcactaaa gggatgtgtc gatatataca
     7561 acccaaaagt aattaggtct actatgggtt ctatttttga tatgaatata attgatgctt
     7621 cacaagatga aactgtggac atgcttaaat cattggattt taatatagtt tcaagttact
     7681 taaatacaga aaatttttat gacaaaatag attatggctc aaaagtagca ttggtgatag
     7741 gaaacgaagc aaatggaata aatgaagaac ttgtatcaaa gtctgatatt ttggttaaga
     7801 tacctatata tggtaaagcc gagtcgttaa atgctgcgat aagctctgct atactgatgt
     7861 atgaaataaa aaaatactta atttaatgta ttgtaataaa tataatgtca tgttataatc
     7921 ttgaatt
//
LOCUS       DBGJFF010000157         7783 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.160, whole genome shotgun sequence.
ACCESSION   DBGJFF010000157 DBGJFF010000000
VERSION     DBGJFF010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7783
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.160"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            237..1937
                     /locus_tag="ACOIAF_003403"
     CDS             237..1937
                     /locus_tag="ACOIAF_003403"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2670396.1"
                     /translation="MIKQFVKYYKPYKKIFTLDLIAAFLFSLCDLVYPMITRNIMDDV
                     VPNKNLRMLVVFAVALILIFIAKAGLNYFMQYWGHVIGVDMQADMRNEVFTHLQRLPN
                     TYFDNNKSGVTMSRIVNDLMDITELAHHGPEDLFISIVMLVGSFFILIDINIPLTLII
                     FAILPFIIWFAIAKKDKMNIAFMKSRVTIGDVNATLENSIAGMKVTKSFCTEKEELNK
                     FVRSNKLFRRARQDSYKVMAEYYSGMNLYMDILEWVVVIAGGYFTYIGKITLGDFAAY
                     ILYVKMFIQPMKKLINFTEQYQNGMTGFKRFIEIMEQDHQKEAKNPIELENVKGDIEI
                     ENISFTYEDKTQVLDNLSLSIKAGKTIALVGPSGGGKTTLCNLLPRFYEFDKGDIKID
                     GKSIKDVSLKSLRKNIGIVQQDVFLFTGTIRDNILCGNPNATDEEMIAAAKKARIHDF
                     VETLPDGYDTYIGERGVKLSGGQKQRISISRIFLKNPPIIILDEATSALDNVTEREIQ
                     ESLEELSKDRTNLVVAHRLTTIKNADEIIVLTDKGIEERGTHEELVNKNGVYSRLHNN
                     "
     gene            2300..3487
                     /locus_tag="ACOIAF_003404"
     CDS             2300..3487
                     /locus_tag="ACOIAF_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670397.1"
                     /translation="MEAITAFCLVLVSLTIGDIVSAKTKAFVPSVFVSAIIFIVGFWT
                     FFPENIVDLAALGTPLAQLGMLLLITHMGTMMSIKELAGQWKTIVIALAGIVGICVGA
                     LALGTVVFGWDTAVIATPPLTGGLVASIMMADAATAKGLASLSVLAILMYVAQGFAGY
                     PITALMLKKEGKRLLSDFRSGKVTVNTDEEKVKDLPEQKSRFKIIPDLPEKYDTTYMI
                     ILRLGFVAWLAVQFTNLTNEVVSRYVICLIFGVIASELGIVDRKPLNKSGSFGWLMTS
                     LMAFIFAGLAKATPAMLVEMAVPLVGIIIFGVIGMGIFSIIVGKLLGESKEMAFSVSL
                     TSLYGFPPNYILTEEAVKALAETPEEKEFLMDKMLPKMLVGGFTTVTIASVVIAGIFV
                     NLI"
     gene            3547..4710
                     /locus_tag="ACOIAF_003405"
     CDS             3547..4710
                     /locus_tag="ACOIAF_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HGZ2670398.1"
                     /translation="MDIKEITKSYKDYVIKLRREFHENPEKSMEEVRTSKRVKEELDK
                     IGIPYVSAGGTGVIATIKGANPGKTVALRGDMDALQVVECTDVEYKSKNEGLMHACGH
                     DGHTSMLLGAAKVLNDIKDSINGTVKLFFQPGEEVGKGARAMIQDGAMEGVDSVFGIH
                     LWTDVESGTISVEEGPRMASADFFKITVKGRGGHGSLPHQGVDAVLASSAIVMNLQSM
                     VSREVSPLEPLVVSVGVLNSGTRFNVIASEAVLEGTIRLFNPELRKQIPGILERIAKS
                     TAEAYRADAELEYGYLTPAVINDKECSKIATDAAIKLFGEDCITLFEKVTGAEDLAEF
                     MNIAPGALAFVGARNESKGACYPHHHGCFNIDEDALEIGTALYVQYAVDFLNK"
     gene            4861..5664
                     /gene="zupT"
                     /locus_tag="ACOIAF_003406"
     CDS             4861..5664
                     /gene="zupT"
                     /locus_tag="ACOIAF_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HGZ2670399.1"
                     /translation="MNNVIFAFLITLLAGLSTGIGSCIAFFAKKTNTKFLSISLGFSA
                     GVMIYVSMIEIFPKAQDALTKSMGEKLGSWSTVIAFFIGMAIIAVIDKLIPQEENPHE
                     IKKMENIEEKNIKKNKSLLRTGIFTAMAIAIHNFPEGLATFISALDDVTIAIPIAIAI
                     AIHNIPEGISVSVPVYYATGDKKKAFYYSFLSGMSEPLGAIIGYVLLRNFLNDITLGI
                     VFAIVGGIMVFISLDELLPSAREYGEHHLSIYGLIAGMGVMAISLLLFK"
     gene            5768..6319
                     /locus_tag="ACOIAF_003407"
     CDS             5768..6319
                     /locus_tag="ACOIAF_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HGZ2670400.1"
                     /translation="MEKKIKFKFDWYSSPDKIEKWLEDIEAKGYNLYKINKFGGIFYF
                     HKGSPRKIKYTVDYYYDVENNYFDEHKSNGWNLVFRSIGSFMGTSAWYIWSKEYKDIP
                     PKLYSDSDKTNKVKSILFMNMFIFFMLVVIFIYLLNIEIRNLYSGYGASVTAIVLQII
                     LLILYAVQLYRRLMYYLRLKKFK"
     gene            6570..7283
                     /gene="rgbR"
                     /locus_tag="ACOIAF_003408"
     CDS             6570..7283
                     /gene="rgbR"
                     /locus_tag="ACOIAF_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgbR"
                     /protein_id="HGZ2670401.1"
                     /translation="MIKIAVCEDEKETQLLIEDYLENILKDISIEYEIQKYISGEELL
                     ESNLKDIDILLLDIKMEKLNGMDTARKIREVDNEMEIIFVTSLIDYVQEGYEVRAYRY
                     LLKPIELEELKKHVLTCIKDIEINKESHITIKNKSNTYKIYLNEIKYIEVQKKDMLIH
                     TINKNFDIKYSLDKIEKELNPYKFIRCHKSFIVNLRYVENIKPNTAILESGEEVPISR
                     YRYKEVKEKFLKFLGDTIC"
     gene            7277..>7783
                     /locus_tag="ACOIAF_003409"
     CDS             7277..>7783
                     /locus_tag="ACOIAF_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HGZ2670402.1"
                     /translation="MLNFMTELLVVLSIFIQFSIMNGILIYKKDSKKNEKIVFYIVLF
                     LIYIIIQISRLFNGGNYFSNINYLFYILAFIAWGGYYNYFYKMGYIKYSIFFYLFSVY
                     YKCLDAVIYRILFFAITGNVISSKESLLVPHLESIKIQIYSNLIIILIYLIFSFFRKS
                     IKLISQDKR"
BASE COUNT         2948 a          791 c         1396 g         2648 t
ORIGIN      
        1 aatttagtat taaatataga gttgtatctt tataaaattt taaatataga gatatagctc
       61 tttttatgtt gtttcaagta tacaactata tgatataaat cttcaatttt atgaaaaaga
      121 ttgttattac tttgtaagtt tataaaagat ttaaagttat tgttatgtta tactaattta
      181 tgggtatttt ttagaagtac taatagaagt actaataaag gagggattta atacttatga
      241 taaaacaatt tgtcaaatat tataagccat ataagaaaat ttttacacta gaccttatag
      301 cagcttttct attttcctta tgtgatttag tttatcctat gataactaga aatataatgg
      361 atgacgttgt tccaaataaa aacttaagaa tgttagtagt ttttgcagta gcacttatac
      421 ttatatttat agcaaaagca gggcttaact attttatgca gtattgggga catgttattg
      481 gtgtagatat gcaagctgat atgagaaatg aggtatttac acacttacaa agacttccaa
      541 atacgtattt tgataataac aaatctggag taactatgtc tagaatagta aatgatttaa
      601 tggatataac agaacttgca catcatgggc ctgaagattt attcatttct atagtaatgt
      661 tggttggttc attctttata cttatagata taaatattcc tcttacgctt ataatatttg
      721 ctatattacc gtttataatt tggtttgcaa tagctaaaaa agataagatg aatattgctt
      781 ttatgaagag tagagttact ataggtgatg taaatgctac tttagaaaat agtatagcag
      841 gtatgaaggt aactaagtct ttttgtactg agaaagaaga gttaaataag tttgtaagaa
      901 gcaataaatt atttagaaga gcaagacaag attcttataa ggtaatggca gaatattact
      961 ctggtatgaa tttatacatg gatatcttag agtgggtagt tgtaatagct ggtggttatt
     1021 ttacttatat aggaaagatt actttgggtg attttgctgc ttatatacta tatgtaaaaa
     1081 tgtttataca acctatgaaa aaattaataa actttacaga gcaatatcaa aatggtatga
     1141 caggttttaa aagattcata gagataatgg aacaagacca tcaaaaagaa gctaaaaatc
     1201 ctattgaact tgaaaatgta aaaggtgaca ttgagataga aaatatatct ttcacatatg
     1261 aagataagac tcaagttttg gataatttaa gtttatctat aaaagctggt aagactatag
     1321 cattagttgg accttctgga ggaggaaaga ctacattatg taatctattg ccaagatttt
     1381 atgaatttga taagggtgat ataaaaattg atggaaagag tataaaagat gttagtttaa
     1441 aatcattgag aaaaaatata ggtatagttc aacaagatgt atttttattt acaggaacta
     1501 taagagataa tattctttgt gggaacccta atgctacaga tgaagaaatg atagctgctg
     1561 ctaaaaaagc tagaatacat gactttgtag agactttgcc agatggatat gatacttata
     1621 taggagaaag aggcgtaaaa ttatcaggtg ggcaaaaaca aagaatttca atttcaagaa
     1681 tctttttaaa gaatcctcca ataataatat tggatgaggc tacatctgct ttagacaatg
     1741 taacagaaag agaaattcaa gagtctttag aggaattaag taaagataga acaaatttag
     1801 ttgttgcaca tagattgact actataaaaa atgctgatga aatcatagta ttgacagata
     1861 aaggaattga agaaagaggt actcatgaag agctagtaaa caaaaatgga gtatatagta
     1921 ggttacacaa taactagtat acaaatttta caccatagct aacatacaca ttaaacttac
     1981 aaacatagga tatattaaga ttcataaaga caattcattt tatatttata agatgtttta
     2041 tgaataaaat gtatcctatg ttttattttt tgcaaattac atttaattct ctttgtaaga
     2101 atagagttaa atagccaatg gtcgggataa cgtttttaca aaaagaataa tttagaaaac
     2161 taactttaat ttatggaatt tatcgaattt tatgaaaaaa atcgaatttt gacttgaaat
     2221 gtttttcaaa agtagtaaaa taatggataa ctatacatac attttataaa aatatattta
     2281 ttatgagggg ggattttgta tggaggcaat aacagctttt tgtctagttt tagtatcact
     2341 gacgatagga gatatagttt cagcaaaaac taaagctttt gttccgtcgg tttttgtatc
     2401 agcaattata tttatagtag gattttggac ttttttccca gaaaatattg tggacttagc
     2461 cgctttagga acaccattag ctcaattagg tatgcttttg ttaataactc atatgggaac
     2521 tatgatgagt attaaggaat tagcaggtca atggaaaaca atagtaatag ccttggcagg
     2581 aatagtagga atatgtgtag gtgctttggc attagggaca gtagtatttg gatgggatac
     2641 agcagtaata gctacacctc ctcttactgg agggctagtt gcatctataa tgatggcaga
     2701 tgctgctaca gcaaaaggtc ttgcaagttt atcagtactt gcaattttga tgtatgttgc
     2761 acaaggattt gcgggttatc ctattacagc attaatgctt aaaaaagaag gaaaaagact
     2821 tttatcagac tttagaagtg gaaaagtaac tgtaaataca gatgaagaaa aagttaagga
     2881 tttacctgaa caaaaaagca gatttaagat aattccagac ttaccagaaa aatatgatac
     2941 gacatatatg ataatactta gattaggatt tgtagcatgg ttagcagtac aatttacaaa
     3001 tcttacaaat gaagttgtat caagatatgt tatatgttta atatttggtg taatagcatc
     3061 agaacttgga atagttgata gaaaaccact taataaatct ggttcatttg gatggttaat
     3121 gacatcattg atggctttta tatttgcagg gttagcaaaa gctacaccag caatgcttgt
     3181 tgaaatggca gttcctcttg taggaatcat aatatttggt gtaataggta tgggaatatt
     3241 ctctattata gtaggtaagc ttttaggtga aagtaaagaa atggcttttt cagtttcgct
     3301 tacatcactt tatggttttc caccaaacta tatattaact gaagaggcag taaaggcttt
     3361 ggctgaaaca ccagaagaaa aagaattttt aatggataag atgcttccaa agatgcttgt
     3421 tggaggattt actacagtta caatagcttc tgtagtaata gcaggtatct ttgtaaatct
     3481 aatatagtat ttaagtgata aaaagtattt taaataagaa gaatgtgtac tacttggagg
     3541 tatggaatgg atattaaaga aataacaaag agttataaag actatgtaat taagcttaga
     3601 agggaatttc atgaaaatcc tgagaaaagt atggaagaag ttagaacatc aaagagagtt
     3661 aaagaagaac ttgataaaat agggatacca tatgtatcag ctggaggtac aggtgtaatt
     3721 gcaactataa aaggagctaa tccaggaaag actgtagcac ttaggggtga tatggatgct
     3781 ctacaagttg ttgaatgcac tgatgtagaa tacaaatcta aaaatgaagg acttatgcat
     3841 gcttgtggtc atgatggtca cacatcaatg cttctagggg cagcaaaagt gttaaatgat
     3901 attaaggata gtatcaatgg aactgtaaaa ttattttttc agccaggaga agaagttggt
     3961 aaaggtgcaa gagctatgat tcaagatggt gctatggaag gtgtagatag tgtgtttgga
     4021 atacatcttt ggactgatgt agaatctggg accatatcgg tagaagaagg accaagaatg
     4081 gcttctgctg atttctttaa aattactgta aaaggtagag gtggtcatgg ttcacttcct
     4141 catcaaggtg ttgatgcagt gcttgcaagt tcagcaatag ttatgaatct acaatctatg
     4201 gttagtagag aagtaagtcc tctggaacca ttagttgtga gtgttggagt tttaaattct
     4261 ggaactagat ttaacgtaat tgcgagtgaa gctgtcttag aaggtacaat aaggctattt
     4321 aatccagagc ttagaaagca aattcctgga attttagaac gtatagcaaa atctacagca
     4381 gaagcatata gagcagatgc tgaattagag tatgggtact taacaccagc agttataaat
     4441 gacaaagaat gttcaaagat agctacagat gcagcaatta agctatttgg agaagactgt
     4501 ataacactat ttgaaaaagt aacaggagca gaagatttag ctgagtttat gaatatagca
     4561 cctggtgcac tagcatttgt aggagctaga aatgagtcaa aaggtgcttg ttatcctcat
     4621 catcatggat gctttaatat agatgaagat gctctagaaa ttggaactgc tttatatgtg
     4681 caatatgcag tggatttctt aaataaataa ttttaatata ctataaagaa aaagcagaga
     4741 ctaagtttat atctctgctt ttttattgtg tatatttttt ataaatattg actatataaa
     4801 atatatatga tagcataaag aaaatgataa tcaatatcaa ttaaaaagaa ggtgatttat
     4861 ttgaataatg taatatttgc atttttgata acattattgg ctggtttatc tactggtata
     4921 ggaagttgta tagcattttt tgctaaaaaa acaaatacta agtttctttc tataagctta
     4981 ggattttctg ctggtgttat gatttatgtt tctatgatag aaatttttcc taaagcacaa
     5041 gatgctttga caaaatctat gggagagaaa ttaggaagtt ggtcaactgt aatagctttt
     5101 tttataggta tggctatcat tgctgttatt gataagctaa ttccacaaga agaaaatcca
     5161 catgaaataa agaaaatgga gaatatagaa gaaaagaata tcaaaaaaaa taagtctctt
     5221 ttaagaacag gtatatttac agcaatggct atagcaattc acaattttcc tgaaggatta
     5281 gctacattta tatcagctct tgatgatgtt acaatagcta ttccgatagc aattgcaatt
     5341 gcaatacaca atataccaga aggtatatct gtatctgtac cagtttatta tgccacagga
     5401 gataaaaaga aagcttttta ttattccttt ttatctggta tgtctgagcc tttaggagcg
     5461 attattggat atgtacttct tagaaatttt ttaaatgata taactcttgg aattgtattt
     5521 gcaattgtag gaggaattat ggtattcata tcactagatg aattattgcc atcagctaga
     5581 gagtacgggg aacatcattt atctatttat ggattgattg caggaatggg tgttatggct
     5641 attagtttat tactatttaa atagctttta aatatatgat tgtaacctaa aattagtatc
     5701 aaaatgatat aattatttaa atagattttt aagctattta aaaatatcat tttggagaat
     5761 gagtgctatg gaaaagaaaa taaaattcaa atttgactgg tatagttcac ctgataagat
     5821 agagaaatgg ttagaagata ttgaagctaa aggatataat ttatataaga taaataaatt
     5881 tggaggaata ttttattttc ataagggaag tccaagaaaa ataaaataca ctgttgatta
     5941 ctactatgat gtagagaata attattttga tgaacataaa tcaaatggct ggaatcttgt
     6001 atttaggagt attggtagtt ttatgggtac atctgcatgg tatatatggt cgaaagaata
     6061 caaagacata cctcctaaat tatatagtga ttctgataaa acaaataaag taaagtcaat
     6121 tttatttatg aatatgttta tattctttat gttagtagta atatttatat atttattgaa
     6181 tattgaaata agaaatttat atagtggtta tggagcatct gtaactgcta ttgtactaca
     6241 gattatattg ttgattttat atgcagtaca attatatagg agattaatgt attatttaag
     6301 gcttaaaaag tttaaataaa taaaagaagg tgtcctaaaa ttattttttt aatattttaa
     6361 ggcatcttct tttgttaata aagtactgtt tttaattata gtaatttctg tctttaaatt
     6421 gaaatatcaa ataatagtta tatgggattg agtattgata aaaataattt gaaaatgtta
     6481 tcctaaagta tcagatatac aaaattatct ttaaatatat tataaatatt ttaattgtat
     6541 taactagaaa ttatgtaagg aggcatttta tgattaagat agcagtttgt gaagatgaaa
     6601 aagaaacaca acttttaata gaagattacc ttgagaatat attaaaagat ataagtatag
     6661 aatatgagat acaaaagtat atatctggag aagagttact ggaaagtaat ttaaaagata
     6721 tagatatatt acttcttgat ataaaaatgg aaaaactaaa tggaatggat actgcaagaa
     6781 agattagaga agtagacaat gaaatggaaa taatatttgt aacttcatta atagattatg
     6841 tgcaagaagg ttatgaggta agagcttata ggtatttact aaagccaata gagttggaag
     6901 aactaaaaaa acatgtgcta acttgtatca aagatattga aataaataag gaaagtcata
     6961 ttacaataaa aaataagtct aatacatata agatttattt aaatgaaata aaatatatag
     7021 aagttcaaaa aaaagatatg ctaatacaca caataaacaa gaattttgat ataaaatata
     7081 gtttagacaa aatagaaaaa gaattaaatc catataaatt tataagatgc cataaaagtt
     7141 ttatagtaaa tttaagatat gttgaaaata taaagcctaa tactgcaata ctggaaagtg
     7201 gagaagaggt acctattagt agatatagat ataaagaagt taaggagaaa ttcttaaaat
     7261 ttcttggtga tacaatatgc taaactttat gactgagcta ttagtagttt tatctatatt
     7321 tatacagttt tctataatga acggaatttt aatttataaa aaagatagta aaaaaaatga
     7381 aaaaatagta ttctatattg tcttgtttct tatttatatt ataattcaga taagtagatt
     7441 atttaatgga ggaaattact ttagtaatat aaactattta ttttatatat tagcatttat
     7501 tgcttgggga ggatactata attattttta taagatgggt tatattaagt attccatttt
     7561 cttttatcta tttagtgtat attataaatg tttggatgct gttatatata gaatactatt
     7621 ttttgcaatt acaggtaatg ttatttcatc taaagaatct ttattagtac ctcacttaga
     7681 atctataaaa attcagatat atagtaattt aataattatt cttatatatc ttatatttag
     7741 cttttttaga aagtctataa aattaattag ccaagataag aga
//
LOCUS       DBGJFF010000158         7720 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.161, whole genome shotgun sequence.
ACCESSION   DBGJFF010000158 DBGJFF010000000
VERSION     DBGJFF010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7720
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.161"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            77..1135
                     /locus_tag="ACOIAF_003410"
     CDS             77..1135
                     /locus_tag="ACOIAF_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HGZ2670403.1"
                     /translation="MNCKWISKIDERKKCHREADSSGYCIFHKENKSDEEIQLMMDTL
                     HKEEISEFNGFVFENEFNAEEILTYNYKILDFSESIFKQKANFKKYIFKKNIIFNYTE
                     FRDKVLFNGCVFLENCDFNRTIFSKHYINDRIFEKVKFKGPDLVVNKVENFPRMDGII
                     FSMCTKFVLKNVEYGKSEYEHGKINYRIARNQATKIGEYEMIGFYYYKERIYSSKIMK
                     RSNYPTFSDYLVEKFFDQIARYTTGYGEKPWNILLVIIAIISVFALLYLFVGIESSNS
                     TLVALDINNIGDYSLSEIFKMYMDLWYFSMATFSTVGYGDMVATSLIGKALAGIEVFF
                     GVTIGAIWASVIIKRMIR"
     gene            1239..2423
                     /locus_tag="ACOIAF_003411"
     CDS             1239..2423
                     /locus_tag="ACOIAF_003411"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HGZ2670404.1"
                     /translation="MKNALKKIISLAMILVFIIPNYSYADDKSVDKYSKTSILIDQET
                     GRVLYSKEPDMKVPLASLSKMMTFLLAIEAIENKEVKETDKVKIDKSIASVKGSSYKL
                     KDGEEVPLIELMRGLMIVSGNDAAIAIAKHICKTKEAFVDRMNKKAKEIGMSNTHFLN
                     PNGLPIYDLSNPKKPAKENISTAKDIAILGKYMFDHYEKQVTAITDMETYTNSDRSFE
                     KSNTNALLRIIPEVDGIKTGYTGNAGYCLSFSMVVNKNDKNEKKRRVIGVVLGTNHKN
                     KRTSASLALLKYGKENFNMKKVIDKDSFIGKKYIKGMKELEVTLKAKDELYTILKDDE
                     SIKSEVKIKNIEYPVKKGDTMGIIKYYTNSGDLLGSVDIVSNNSIDNVSLKTKLKMKF
                     AN"
     gene            2630..3343
                     /gene="rpsB"
                     /locus_tag="ACOIAF_003412"
     CDS             2630..3343
                     /gene="rpsB"
                     /locus_tag="ACOIAF_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HGZ2670405.1"
                     /translation="MSVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIYIIDLQ
                     KTVKKVEEAYKFTKEVAETGKPILFVGTKKQAQDAIKDEAERCGMYFVNERWLGGMLT
                     NHKTIKTRINKLRELEKMEEEGVFNVLPKKEVIKLRAEKEKLEKYLGGIKDMPELPGA
                     MFVVDPRKENIAIQEAHRLGIPVVGIVDTNCDPEQLDFAIPGNDDAIRAVKLITGAMA
                     TAVIEGRQGAEEEVAEDQE"
     gene            3422..4333
                     /gene="tsf"
                     /locus_tag="ACOIAF_003413"
     CDS             3422..4333
                     /gene="tsf"
                     /locus_tag="ACOIAF_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HGZ2670406.1"
                     /translation="MANITAQMVKELRESTGAGMMDCKKALQEAEGNMEKAVDLLREK
                     GLSKAAKKAGRVAAEGLVAIEMNDDNTVASMVEVNSETDFVAKNEDFKVFVKDAACMA
                     LATDKEDIASLLGETHKEGITLQEVLNNRVAKIGEKLDFRRFAKVVTNGQVAGYIHGG
                     GKIGVLVEMETEARDAKVLELGKDVAMQVAAMNPKYVSRDEVDAEYIAHETEVLTQQA
                     LNEGKPANIVEKMVKGRLEKELKEVCLLEQTFVKNPDITVKQLVADVAKAVGSDIKVV
                     KVVRFEVGEGIQKREENFAEEVAKQLK"
     gene            4418..5122
                     /gene="pyrH"
                     /locus_tag="ACOIAF_003414"
     CDS             4418..5122
                     /gene="pyrH"
                     /locus_tag="ACOIAF_003414"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HGZ2670407.1"
                     /translation="MDKPMYKRVLLKLSGEALAGERGFGINNDVVNDIAIAIKKIQEI
                     GVEVAVVVGGGNFWRGRTSEGMDRTTADYIGMLATVMNAMALQDALENIDVATRVQTA
                     IDMRQIAEPYIRRRAVRHLEKERVVIFGAGTGNPYFTTDTTAALRAAEMEAEVILLAK
                     NVDAVYDKDPKVHADAKKFTELSYMEVIQKELKVMDSTATSLCMDNKIPIKVFELTTE
                     NIIRAVKGENIGTTVK"
     gene            5148..5705
                     /gene="frr"
                     /locus_tag="ACOIAF_003415"
     CDS             5148..5705
                     /gene="frr"
                     /locus_tag="ACOIAF_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HGZ2670408.1"
                     /translation="MKLEIHKQLEEKMNGTIDALKFEFGTIRAGRANAQMLDKIRVDY
                     YGTPTPINQIGAISVPEPRILMISPWDKSAMHEIEKAIANSDLGLNPSNDGEVIRLSV
                     PALTEERRKELAKKASKAAEEFKVRIRNERRDANEKIKKMEKGGELTEDELKKAQDEV
                     QKMTDKFIKEIDTLLSKKEKDIMEV"
     gene            5708..5911
                     /locus_tag="ACOIAF_003416"
     CDS             5708..5911
                     /locus_tag="ACOIAF_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670409.1"
                     /translation="MKKYYNLLGLHVDEVKQYFDEQNINYTVKSIEGKKDKEKLVVPR
                     VIKISEIGDSVELIITYFSDSLV"
     gene            5940..6674
                     /locus_tag="ACOIAF_003417"
     CDS             5940..6674
                     /locus_tag="ACOIAF_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HGZ2670410.1"
                     /translation="MNNNIIYDIDLNNIPTHIAIIMDGNGRWAKARFLPRTAGHKAGV
                     ETIRDIVKECSKLGVKHLTLYAFSTENWKRPKLEVDTLMNLLSTYLRNEIAELHQNNV
                     KVTAIGDISALPKTCIRELNSAKELTKNNTGVNLNLALNYGSRADIKNALIDIVKNCE
                     SGKIDINNIDEDIIKNYLSTKSIPDPDLVIRTSGEQRLSNFLLWEVAYSEFYFTDIHW
                     PDFKKEELQKAIYVYQRRDRRFGGLK"
     gene            6687..7466
                     /locus_tag="ACOIAF_003418"
     CDS             6687..7466
                     /locus_tag="ACOIAF_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HGZ2670411.1"
                     /translation="MLTRIIASLALVPLFLFVVYGGIPLYIAETAIVYIALHEFYKAF
                     KIKDVHPIFIIGYLFSIYLAVKNIFNLPLEYTYAVIFILFLASIIYMLMGKNNVIDVS
                     ITFLGVFYIGVFLDFIIITINGFEKGSIYVWLIFVISFMTDIFAYFSGYLLGKHKLIP
                     KVSPKKTIEGAIGGIIGSTLCCILFGYLFGIDLLQLAIIGSIGSVIAQLGDLFASSIK
                     RYVGIKDYGKIIPGHGGILDRFDSVILVAPFVYSAIKFFIR"
BASE COUNT         3180 a          789 c         1459 g         2292 t
ORIGIN      
        1 taagtatata aaataaaata atatccaaac taatacaaaa tagtaataat tgttttgtac
       61 tggaggatat atatttatga attgtaaatg gatatctaaa atagatgaga gaaaaaaatg
      121 tcatagagaa gcagattctt ctggatattg tatctttcat aaagaaaata agtctgatga
      181 agagatacag ttgatgatgg atactcttca taaagaagag ataagtgaat ttaatggatt
      241 tgtttttgaa aatgaattta atgcggaaga aatattaact tacaattaca aaatactgga
      301 tttttctgaa tccatattta aacaaaaagc aaattttaaa aaatatattt ttaaaaagaa
      361 tattattttt aactatacag aatttagaga taaagtttta tttaatggtt gtgtattctt
      421 agaaaactgt gattttaata gaaccatatt tagtaaacac tatataaatg accgtatatt
      481 tgaaaaagtg aaatttaaag gacctgattt ggtagttaat aaagtagaaa attttccacg
      541 aatggatgga ataattttta gcatgtgtac aaaatttgtg ttaaagaatg ttgaatatgg
      601 aaaaagtgag tatgagcatg gcaagataaa ttatagaata gctagaaatc aagcaacaaa
      661 aattggagaa tatgagatga taggatttta ctattataag gaaagaattt atagtagtaa
      721 aataatgaaa cgttctaatt atcctacttt cagtgattat ttagttgaaa aattttttga
      781 ccaaatagca cgttacacaa caggttatgg agaaaagcca tggaatatat tattagttat
      841 tatagcaata ataagtgttt ttgctttatt atacttattt gtaggcatag aaagttcaaa
      901 tagcacactt gtagctttag atataaacaa tataggtgat tactcactaa gtgagatttt
      961 caaaatgtat atggatttat ggtattttag catggctaca tttagtacag taggatatgg
     1021 agatatggtt gctacaagtt tgattgggaa agcattggca ggtatagaag tattttttgg
     1081 agttacaata ggagcaattt gggcatctgt gattattaaa agaatgataa ggtaatattt
     1141 gtgtaaatat ggataggtat taattactag atataaattg gactaaatag ggaaaattta
     1201 aatataaata tacttaataa tccaagaagg tgaaaaattt gaaaaatgca ctaaaaaaaa
     1261 ttatatcatt agccatgatt ctagttttta taataccaaa ttattcatat gcagatgata
     1321 aatctgtaga caagtattca aaaacatcta tacttataga ccaagaaaca ggtagagttt
     1381 tatatagtaa agaacctgat atgaaggtac cattagctag tttaagtaag atgatgactt
     1441 ttttacttgc aatagaagct attgaaaata aagaagtaaa agaaactgat aaagtaaaaa
     1501 tagacaaatc tattgcatca gtaaaagggt caagttataa acttaaagat ggtgaagaag
     1561 tgccacttat agagcttatg agaggtctta tgattgtatc aggaaatgat gcggcaattg
     1621 ctatagcaaa acatatttgt aaaacgaaag aagcatttgt agatagaatg aataaaaaag
     1681 caaaagaaat aggaatgagt aatacacatt ttctaaatcc aaatggtctt ccaatatacg
     1741 atttaagcaa ccccaaaaaa ccagcaaaag aaaatatctc tactgcaaaa gatatagcta
     1801 ttcttggaaa atacatgttt gaccattatg aaaaacaagt aacagctata actgatatgg
     1861 agacatatac aaattcagat agaagttttg aaaaaagcaa tacaaatgca ttattaagaa
     1921 ttataccaga agtagatggt ataaaaactg gatatactgg caatgctgga tattgtttat
     1981 cattttctat ggttgtaaat aaaaatgata aaaatgagaa aaaacgtaga gtaatagggg
     2041 ttgtattagg tactaaccat aaaaataaaa gaacctctgc ttcattagct ctactgaaat
     2101 atggaaaaga aaatttcaat atgaaaaaag taattgataa agatagcttt attggtaaga
     2161 aatatataaa aggaatgaaa gagttagagg ttacattaaa agcaaaagat gaactttata
     2221 caatattaaa agatgatgaa agtataaaat cagaagttaa gattaaaaat atagagtacc
     2281 ctgttaaaaa aggtgatact atgggtatta taaaatatta tactaattca ggtgacttac
     2341 ttggaagtgt agatatagta agtaataata gtatagataa cgtatcatta aaaacaaaat
     2401 taaaaatgaa atttgcaaat taaattcaaa aaatggtata aaatacacaa aaaaatataa
     2461 aaatagaaaa tatggaaaaa atattgttat ttcttcacct ctgtgatata ctactagagg
     2521 tgaaaaaata aaataaatca cacacagcat tgggatcctt aaaaggtgcc tattacatgg
     2581 ttttttaggg agaagatcaa gctggaggta taaaaccagg aggtaagata tgtcagtaat
     2641 atcaatgaaa caattattag aagcaggtgt tcactttggt caccaaacaa gaagatggaa
     2701 ccctaaaatg gctaagtata tattcacaga gagaaatgga atatatataa tcgacttaca
     2761 aaaaacagtt aaaaaagttg aagaagctta caagttcact aaagaagtag ctgaaacagg
     2821 aaagccaatc ttattcgtag gaactaaaaa acaagctcaa gatgcgataa aagatgaagc
     2881 tgaaagatgt ggaatgtact ttgttaatga aagatggtta ggtggaatgt taacaaacca
     2941 caaaactata aaaacaagaa taaataaatt aagagaatta gaaaaaatgg aagaagaagg
     3001 cgtatttaat gttcttccta aaaaagaagt tataaaatta agagctgaaa aagaaaaact
     3061 agaaaaatac ttaggtggaa taaaagacat gcctgaatta ccaggtgcaa tgttcgtagt
     3121 tgacccaaga aaagaaaaca tagctattca agaagctcat agattaggta taccagtagt
     3181 tggtatagtt gatactaact gtgacccaga acaattagac tttgctatac caggaaatga
     3241 tgacgctata agagccgtaa aattaataac tggtgctatg gcaacagctg taattgaagg
     3301 tagacaaggt gctgaggaag aagtagctga agaccaagaa taataatttg acaattaaat
     3361 ggtaagggtc gcccttacca tttataatat ggaatagaat aagtaggagg aaacttaaaa
     3421 aatggctaat ataactgctc aaatggtaaa agagttaaga gaaagtacag gcgcaggaat
     3481 gatggactgc aagaaagctt tacaagaagc tgaaggaaac atggaaaaag cagtagattt
     3541 attaagagaa aaaggattat caaaagctgc taaaaaagct ggtagagttg ctgctgaagg
     3601 tctagttgca atagaaatga atgatgataa tacagttgct tctatggtag aagttaactc
     3661 agagacagat ttcgttgcta aaaacgaaga cttcaaagtg ttcgttaaag atgctgcttg
     3721 tatggcatta gctactgata aagaagatat agcttcttta ttaggtgaaa ctcataaaga
     3781 gggaataact ttacaagaag ttttaaataa tagagttgca aaaataggtg agaaattaga
     3841 ctttagaaga tttgctaaag ttgtaactaa tggacaagtt gctggataca ttcacggtgg
     3901 tggaaaaata ggtgttcttg ttgaaatgga aacagaagct agagatgcta aagttttaga
     3961 attaggtaaa gatgtagcta tgcaagttgc tgctatgaat cctaagtatg tttcaagaga
     4021 tgaagtagat gctgaataca tagcacatga aacagaagta ttaactcaac aagctttaaa
     4081 tgaaggaaaa ccagctaata tagttgaaaa aatggttaaa ggaagattag aaaaagaatt
     4141 aaaagaggtt tgtttattag agcaaacttt cgttaaaaat ccagatataa ctgttaaaca
     4201 attagttgct gatgttgcta aggcagtagg ttctgatata aaagttgtta aagttgtgag
     4261 attcgaagtt ggtgaaggta tacaaaagag agaagaaaac tttgcagaag aagttgctaa
     4321 gcaacttaag taataaccaa agagagaaca cgcaggtgtt ctctttttta aaaaatagaa
     4381 aaaattggat atgaaggatt gaaataggag gtttctgatg gataaaccaa tgtacaaaag
     4441 ggtattgtta aaattaagtg gagaagcatt agcaggagaa agaggttttg gtataaataa
     4501 tgatgtagtt aatgatattg ctattgctat aaaaaaaata caagaaatag gtgttgaagt
     4561 agctgtagta gtaggtggag gaaacttctg gagaggcaga accagtgaag gaatggacag
     4621 aactactgct gactatatag gaatgttagc tacagttatg aatgcaatgg cgctacagga
     4681 tgcactagaa aatatagatg ttgctacaag agttcaaact gctatagaca tgagacaaat
     4741 tgcagaacca tatataagaa gaagagcagt tagacactta gaaaaggaaa gagttgttat
     4801 atttggtgca ggaacaggaa acccttactt tacaacagat acaactgctg ctttacgtgc
     4861 tgctgagatg gaagcagaag ttatattgtt agctaaaaat gttgatgcag tatatgacaa
     4921 ggatccaaaa gtacatgcag atgctaagaa atttacagaa ttaagttaca tggaagttat
     4981 acagaaagaa ttaaaagtaa tggattctac agctacatct ttatgtatgg ataataaaat
     5041 tccaataaag gtatttgagc ttacaacaga gaatataata agagcagtaa aaggcgaaaa
     5101 tataggaact actgtaaaat aatgaagaat gaaggaggag taaaattatg aaactggaaa
     5161 tacataagca attagaagag aaaatgaatg ggacaattga tgctttaaag tttgaatttg
     5221 gaactataag agcaggaaga gctaatgcac aaatgctaga caaaataaga gttgattatt
     5281 atggaactcc aactccaata aatcaaatag gtgctatatc tgtaccagaa ccaagaatat
     5341 taatgatatc tccttgggat aaatctgcta tgcatgaaat agaaaaagcg atagctaact
     5401 cagacttagg attaaatcca tcaaatgatg gtgaggttat aagactttct gtacctgctt
     5461 taacagaaga aagaagaaaa gaattagcta aaaaagctag taaagctgca gaagaattta
     5521 aagttagaat aagaaatgaa agaagagacg ccaatgagaa gattaaaaag atggaaaaag
     5581 gcggagaatt aactgaagat gaattaaaaa aagcacaaga tgaagttcaa aaaatgactg
     5641 ataaatttat aaaagaaata gatacattat tatctaaaaa agaaaaggat ataatggagg
     5701 tataatattg aaaaaatatt ataacctttt aggtcttcat gtagatgagg taaaacaata
     5761 ttttgatgag caaaatataa attataccgt aaaatctatt gaaggtaaaa aggataaaga
     5821 aaaacttgta gttcctagag ttataaaaat atctgagata ggtgatagtg tagaactgat
     5881 aatcacatac ttttctgact ccttagttta aggagtcaga acaatattgg aggcaaaata
     5941 tgaataacaa cattatatat gatatagact taaataacat acctactcat attgctatta
     6001 taatggatgg aaatggaaga tgggcaaaag ctagatttct tccaagaact gcaggacata
     6061 aggctggagt agaaactata agagatatag taaaagaatg ctcaaaatta ggtgttaagc
     6121 acttaacatt atatgcattt tcaacagaaa actggaaaag accgaaactt gaagttgaca
     6181 cacttatgaa cttattatct acatacttaa gaaatgaaat agctgaactt caccaaaaca
     6241 atgtaaaagt aactgctata ggagatataa gtgctttacc aaaaacttgt ataagagaat
     6301 taaactcagc taaggaactg acaaaaaaca atacaggagt taacttaaat ttagcattaa
     6361 actacggaag tagagcagat ataaaaaatg ctcttataga tatagtaaaa aattgtgaaa
     6421 gtggtaaaat agatataaac aatattgatg aagatattat aaaaaactat ttaagcacaa
     6481 agtctatacc agatccagac ttggtaataa gaacaagtgg tgaacaaaga ttgagtaact
     6541 ttttactttg ggaagttgct tattctgagt tttactttac tgatatacat tggcctgatt
     6601 ttaaaaaaga agaactacag aaggctatat acgtatatca aagaagggat agaagatttg
     6661 gaggacttaa gtaaggagat tattttatgc ttacaagaat tattgcttca ctagctctgg
     6721 ttccattatt tctatttgtt gtatatggag gtattcctct atatatagct gagacagcaa
     6781 tagtttatat tgcattgcat gagttttata aggcatttaa aataaaagat gtacatccaa
     6841 tattcattat aggatattta ttttctattt atttagctgt aaaaaatatt tttaatttac
     6901 cattagaata tacttatgca gtaattttta ttttattttt agcaagtata atttatatgt
     6961 tgatgggcaa aaacaatgtt atagatgttt cgattacgtt tttaggagtt ttttatatag
     7021 gagtattttt agattttata attataacta taaatggatt tgaaaaaggt agtatatatg
     7081 tatggcttat atttgtgata tcttttatga cagatatatt tgcatatttt agtggttatt
     7141 tacttggaaa acataagcta attcctaaag ttagtcctaa aaagactata gaaggagcta
     7201 ttggaggaat aataggaagt acactttgtt gtattttatt tggatattta tttggcattg
     7261 atttgcttca attagcgata ataggaagta taggaagtgt aatagctcaa ttaggagatt
     7321 tatttgcatc ttctataaag agatatgtag ggataaagga ttatggaaag ataatacctg
     7381 gtcatggggg tatattagat agatttgata gtgttatatt agttgcacct tttgtatata
     7441 gtgcgattaa attttttatt agataaagat tggttggatt taaaagagca atctataatt
     7501 gctagtaatg aaaatagcta tgtaactttt aaatgcagag atattctgca gttattagtt
     7561 acatggctat tttcattgtt caaaaattta gaagaccttg gggaattgta taagaatagg
     7621 tattacttta taatagtata ctatatttgt agtgagtata ttttttatac tataactatc
     7681 attaaaaaat atttttttgt ttaataattt cgatattttt
//
LOCUS       DBGJFF010000159         7716 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.162, whole genome shotgun sequence.
ACCESSION   DBGJFF010000159 DBGJFF010000000
VERSION     DBGJFF010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7716
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.162"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            270..1571
                     /locus_tag="ACOIAF_003419"
     CDS             270..1571
                     /locus_tag="ACOIAF_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HGZ2670412.1"
                     /translation="MGKSKDVEIEKSILGTILLDNKLSYKLDELNENMFMNDICLEIF
                     KIMKELKKENIVIDVATVKSKIDRKSLAIKTSDVTNLITWGQNFGLDGHIKILKENLA
                     RRSINQNCQNLLHSLNLGENIDTCIYKFESNIKEILDKDTYENDDVNSIAGKVLDFLE
                     NKKDIGFKFGIKLLDTTIGGLFKGELTTIAAKSGVGKTALALQIMLNSFKQGKKTLFI
                     SREMTSEQVFMRNICRVTGVSTRNMKSKEIDENDWKLIVNAIGDLSENNLIYINDKID
                     TISAIRKRIRQVKPDLLIVDYVQLLTSQKSMDKREREVATFSRELKNMTLDFNIPVIQ
                     LSQLNDEMKDSRPYGDRPMRDSKAIYHDSNNVVYIHQLKGSDYEEAVRDIGESEEAVR
                     ASEYRGIKMVDLIVAKCRDGQTRHKHFCYFGDKLHFQELNY"
     gene            1647..1922
                     /locus_tag="ACOIAF_003420"
     CDS             1647..1922
                     /locus_tag="ACOIAF_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HGZ2670413.1"
                     /translation="MLELTKIIELLEEHKIEVSIPKELHKYSLLEYLELCQQSGGTYF
                     YIKTLEGALKEARNELMIKLFNGKNYMELARMFEMSVINVRHIVRKK"
     gene            3310..3540
                     /locus_tag="ACOIAF_003421"
     CDS             3310..3540
                     /locus_tag="ACOIAF_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGZ2670414.1"
                     /translation="MSNKKKKYANILANECVACGSCIKACPRSAISVPCGISAKINRD
                     LCVGCGICEKICPASVIEIITILKEEEGKCHE"
     gene            3533..4114
                     /locus_tag="ACOIAF_003422"
     CDS             3533..4114
                     /locus_tag="ACOIAF_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HGZ2670415.1"
                     /translation="MNNKKHWYDYLWIFSSIYLILGFVNIIFAWIGLICFFVPLAISI
                     VKGNKAYCNKYCGRGQLFNLLGNKLKLSRNRDIPKFIRSKWFRYGFLTFFMVMFANML
                     FSTYLVFEGSRNFKEVVTLLWTIKMPWQWAYHGTLVSPWVVQFAFGFYSVMLTSTVLG
                     LITMILFKPRSWCVYCPMGTMTQMICKAKSKSV"
     gene            complement(4693..5370)
                     /locus_tag="ACOIAF_003423"
     CDS             complement(4693..5370)
                     /locus_tag="ACOIAF_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HGZ2670416.1"
                     /translation="MISQNEIDILTHSLPFWDKLTDLQKELLISSANTSHYKKGNPVH
                     CGDSDCIGILIIKSGTVRTYILSDEGREVTLFRLDDGDVCILSASCILKTITFDVYVD
                     AETDCDIIQISSSVFAKLSTENIHAELFSYKLATERFSDVMWAMQQILFMSFDKRLAS
                     FLIDEIAKNGSSTINMTHEQIAKYMGSAREVVSRMLKYFAREGIVSLSRGGIKVLDKD
                     KLRSLTL"
     gene            complement(5559..6923)
                     /locus_tag="ACOIAF_003424"
     CDS             complement(5559..6923)
                     /locus_tag="ACOIAF_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HGZ2670417.1"
                     /translation="MKESNKKIELLGSAPISKALLSMGLPTMIGMMITALYNLVDAYF
                     VGGLGTSQMGAISVAFPLGQLVVGLGLLFGNGAASYVARLLGHGDNEKAGKVVSTSIY
                     SSIIVGTLVIIVIMIFLNPILKLLGATKSIMPYAISYASIYVVSSIFNIFNVTMTNIV
                     TSEGAAKTTMCAMLLGALLNMILDPIFIYTFGLGVRGASIATAISQIASTLVYVGYIL
                     SKKSIFSFRINECCFSKEIFSEILKIGIPTLIFQLLTSLAITLTNMEATKYGDSVIAG
                     MGAVTRITSLGSLVVFGFIKGFQPIAGYNFGSKNYDRLHIAIKTSILWTTVFCVVLGL
                     ILILFPKSIISQFTSGDILLIENGSKALRANGFSFMLFGFYTVYSTLFLALGKAKEGG
                     FLGMCRQGVCFIPIILIIPVFYGLNGILYAQPIADLMAAIIAGFMSIRLHKELNLLKI
                     NGMH"
     gene            7051..7572
                     /locus_tag="ACOIAF_003425"
     CDS             7051..7572
                     /locus_tag="ACOIAF_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HGZ2670418.1"
                     /translation="MATIDLIVLGIVKQQPKSAYDIQKAVEYRNISRWVKISTPSIYK
                     KVIQLEQKGYIESKTVKNGKMPEKAVYSLTELGETHFLDLMFDTSSKPINILLDFNAV
                     IVNLNSLTPEKQKLCLNNIEENVQELKKTIEENISIKTEVPETAKAVLEQQLVLAQAL
                     ETWINSLKDNMGN"
BASE COUNT         3006 a          869 c         1333 g         2508 t
ORIGIN      
        1 gagtaatgaa ttatattgaa caatatatta ataatttgta gtaaagtata tcaaataaga
       61 tattgctatt atgtagtaaa ttatataaaa caatgtatca gaataaatag tatattaata
      121 atttgtagta tatcatgacg aataatacag tcataacaaa tagtgagtca taacaaataa
      181 tgtatttata attgataatg tatttataat tgataatgta tttaaaaatc aaaatataaa
      241 aacttataag gaaagagaga ttaatttata tgggtaaatc aaaagatgtt gaaatagaga
      301 agtctatttt aggcacaatt cttttagaca ataaattaag ctataagcta gatgaattaa
      361 atgaaaacat gtttatgaat gatatatgtc ttgagatatt taaaattatg aaagaactta
      421 aaaaagaaaa catagtgatt gatgtggcca ctgtgaaatc aaagatagat agaaaatctc
      481 tagcaataaa aacaagtgat gtgactaatc taattacttg gggtcagaat tttggtcttg
      541 atggtcatat taaaatttta aaagaaaatt tagctagaag aagcataaat caaaattgcc
      601 aaaacctttt acacagccta aaccttggag aaaatatcga tacttgtatt tacaaatttg
      661 aaagtaatat aaaggagatt ttggacaagg atacttatga aaatgatgat gtaaatagta
      721 tagcagggaa ggtgcttgat tttttagaaa acaaaaaaga tattggattt aaatttggta
      781 taaaattact tgataccact ataggaggtc tttttaaagg agagctaacc acaatagcag
      841 ctaaatcggg ggttgggaag actgctttgg cacttcaaat aatgttaaat tcatttaagc
      901 aaggtaaaaa aactttattt ataagtagag aaatgaccag tgagcaagta tttatgagaa
      961 atatatgcag agtaactggt gtaagtacaa ggaatatgaa atctaaagaa atagatgaaa
     1021 atgattggaa acttattgta aatgctatag gtgatttaag cgaaaataac ttaatttata
     1081 tcaatgataa gatagataca attagtgcaa ttagaaagag gataaggcag gtaaagccag
     1141 atttattaat agtggattat gtgcaactcc ttacatctca gaaaagcatg gataagagag
     1201 aaagggaagt agctactttt tcgagagaac ttaaaaatat gactttagat tttaatatac
     1261 ctgtaatcca attaagtcag ttaaatgatg aaatgaaaga ttcaagacct tatggagata
     1321 gacctatgag agatagcaaa gccatctacc acgatagcaa caatgtggtt tatatacatc
     1381 aattaaaggg gagtgattat gaagaagcag tgagagatat tggagaaagt gaggaggcag
     1441 ttagagcatc agaatacagg ggaattaaga tggttgattt aatagttgcg aagtgtagag
     1501 atggacagac taggcataag catttttgtt actttgggga taagttacat tttcaagaac
     1561 taaattatta ggtcaataaa gtagtaataa caagcttatt aaaagtcggg gaatagcaaa
     1621 tagattataa aatatgaggt gaaaatatgt tggaattaac aaaaattata gaattacttg
     1681 aagaacacaa gatagaagta agtattccaa aggagttaca taagtatagt ttattggaat
     1741 accttgaatt gtgtcaacaa agtgggggaa catattttta tattaaaaca ctggaagggg
     1801 ctttaaaaga ggctagaaat gagcttatga ttaaattgtt taatgggaaa aattatatgg
     1861 aattagcgag gatgtttgaa atgagtgtta ttaatgtcag gcatattgtt agaaaaaaat
     1921 agatattttt ggagaataca agtgtgatat acttctgaaa tagaagttat tatagttgaa
     1981 ataaaattgt ggttaataaa ttaaagttgt gtataagttt aagattatag aggagtataa
     2041 gtgtgattaa cagcatgatt ttgtgaataa aattgaggat atatatgagt taaatgtgtg
     2101 attaataagg taaggttgtg tataagatta tgagtattta aaaggagggg ctataattaa
     2161 tagtgtagag ttgtgtgttt gcttaagatt atataataat ttaatagatt aaactatact
     2221 aataaggtaa aatcgtgtgt ttagttaaag cttattataa aatcttgata tgtgattaat
     2281 gatgcaatat gtgaataaag ttaagattat aatagattta acctgtggct aatagaataa
     2341 aattgtgaat aaagttaaga ttataatagg tttaacctgt gattaataga gtaagagtgt
     2401 aaatagagat aaacttcaat atgagcttaa cctatgatta atagagtaag agtgtaaata
     2461 gagataaact tcaatatggg tttaacttgt gattaataga gtagggtgta actaaagata
     2521 aacttcaata tgagtttaac ttgtgattaa tagagtaagg tgcaactaaa gataaacttc
     2581 aatatgagct taacctatga ttaatagagt aagaatgtaa atagagataa acttcaatat
     2641 gggcttaact tgtggttaat agagtaagag tgtaactaaa gataaacttc aatatgggtt
     2701 taacttgtgg ttaatagagt aagaatgtaa atagagataa acttcaatat gagcttaact
     2761 tgtgattaat agagtaagag tgtaaataga gataaacttc aatatgggct taacttgtga
     2821 ttaatagagt aagagtgtaa ctaaagataa acttcaatat gagcttaacc tatgattaat
     2881 agagtaagag tgtaaataga gataaacttc aatatgggtt taacctgtgg ttaatagagt
     2941 agggtgtaac taaagataaa cttcaatatg agtttaactt gtggctaata gaataagatt
     3001 gtgaataaag ttatagtttt agatgggtat taacctgtta ctaatagaat atgattaggt
     3061 gtaaaattat agagctgtat aagaaaatat tatagatata ttaaatatta gataataaat
     3121 atttatatag atacacaaaa gaaaaggcga tatgaagcta aacttaatta cttcatatcg
     3181 ttttttattt gaaactcctt tgtatttaga gtgtgatttt taatattgag gttttatgtg
     3241 attaagtcac agataaaaag taagtatctt gatataattc ttatagatta ataatatagg
     3301 gaggttatta tgtctaataa aaaaaagaag tatgcaaata ttttagcaaa tgagtgcgtt
     3361 gcttgtggta gctgtataaa agcatgtcca agaagtgcta tctcagttcc atgtggcatt
     3421 agtgcaaaaa ttaatagaga tttatgtgtt ggatgtggga tttgtgaaaa gatatgtcca
     3481 gcatctgtaa ttgagattat aacaatatta aaagaggaag aaggtaagtg tcatgaataa
     3541 taaaaaacat tggtatgact atttatggat tttttcttca atatatttga tattgggatt
     3601 tgtaaatatt attttcgctt ggattgggct aatttgtttc tttgttccac tagctatttc
     3661 tattgttaag ggaaataaag cttactgtaa taagtattgt ggaagagggc aactgtttaa
     3721 tttgcttgga aataaattga agctgtcaag aaatagagat attcctaaat ttattagaag
     3781 taagtggttt agatatgggt ttttgacatt ttttatggta atgtttgcca atatgctttt
     3841 ttcaacttat ttagtgtttg aaggaagtag aaattttaaa gaagtagtga ctttgttgtg
     3901 gactataaaa atgccttggc aatgggcgta tcatggaact ttagtttcac cttgggtggt
     3961 acagtttgca tttggttttt atagtgttat gcttacatca actgttcttg gtttgattac
     4021 tatgatattg tttaaacctc gttcatggtg tgtttattgc cctatgggta ctatgactca
     4081 gatgatatgt aaggcaaaga gtaagtctgt atagaaaggc cttatgtgat atttacagtt
     4141 ttgttcaagt agagatgagt agtaggtttg aatatatatg acaaatatat gagtataggg
     4201 gcatagattt agtttgtgta aggtaaactg atttatgctt cttttttatg tcttagatta
     4261 ttggctaagt ggaaacaatg aatttagata ttaagaatta ttcagttatt tgaagaaaat
     4321 agaataataa ttaatattat atgaatggat attcattaca tataagccag ttaatagcaa
     4381 gatttattta atatgaaata ggtcaagtat ttaaactaaa taaggtcatg acaattaata
     4441 taagtataga tataaaaaga ttagaagtta taacttataa aaggtattct atataagtag
     4501 tatgtggaaa atagtcaaat taacttatta gaaacaaaaa atatacataa aaaataaatg
     4561 taaatatcaa ataagatact atataaaaat agatataagt atctttttta ttattgaaat
     4621 tatgaaaata tattttattt tacacaaaaa agagtgcctc atgaactaaa aagttcattt
     4681 tgagacacac ttttacagtg tcaaagaacg aagtttatcc ttatccaata cttttatacc
     4741 accacgagat aaagacacaa taccttctct agcaaaatat ttcaacattc tcgatacaac
     4801 ttctctggca ctacccatat acttagcaat ttgctcatga gtcatattta tagtgctact
     4861 gccattttta gctatctcat caattaaaaa agatgccaaa cgcttatcaa aactcataaa
     4921 aagaatttgt tgcatagccc acataacatc agaaaaacgt tcggtagcta atttatatga
     4981 aaataactct gcatgaatat tttctgtaga taattttgca aaaacagagg aactaatctg
     5041 tataatatcg cagtccgttt cagcatctac atagacatca aaagttattg tttttagaat
     5101 acaggaagct gataaaatac atacatctcc atcatcaagt cggaacagtg taacctctcg
     5161 accttcatct gaaagtatgt atgtacgcac tgttccagac tttataatca aaataccaat
     5221 acagtcacta tcaccacaat gtactggatt cccttttttg taatgcgagg tatttgctga
     5281 agaaattaat aattcttttt gtaagtcagt aagtttatcc caaaaaggca agctatgtgt
     5341 aagtatatca atttcatttt gtgaaatcat acaatatctc ctttcttaaa ttgttataat
     5401 cccatgatac aagtgaatag ataaattgac aagtagtttg ttaagaggat tatttagtca
     5461 gatagtaata gtatgattta tgtagaaaaa atatgataat atatattctt gctattaatg
     5521 agaaagtaat atgttttgat gatagtaggt ttgtataact aatgcatacc gtttattttt
     5581 aaaagattaa gttccttatg taaccttata gacataaaac cagcaattat agcagccatc
     5641 aaatcagcta ttggttgtgc atataagata ccatttaacc catagaatac aggaataatc
     5701 aaaattatag ggataaaaca gacaccttgt ctacacatac ctaaaaatcc accttccttt
     5761 gcttttccta gtgcgaggaa taatgtagaa tacactgtat agaatccaaa tagcataaat
     5821 gaaaacccat ttgccctaag agctttacta ccattttcta ttaataaaat gtcaccactt
     5881 gtaaattgtg aaattataga ttttgggaag agtatcaata taagaccaag cactacacaa
     5941 aatactgtag tccataatat ggaagttttt attgctatat gtaatctatc gtagttcttt
     6001 gaaccaaaat tataaccagc aattggttgg aaacctttta taaaaccaaa tacaacaaga
     6061 ctacctaaag acgttattcg tgttacagca cccattccag ctatcacaga atcaccatac
     6121 tttgtagcct ccatattagt taatgtaatg gcaagactag tcaataattg aaatattaag
     6181 gtaggtatgc caatttttag gatttctgaa aaaatttctt ttgaaaaaca gcattcgtta
     6241 attctaaaac taaatatact tttcttgctt aaaatatatc ctacataaac aagtgttgaa
     6301 gcaatctgtg agatagctgt tgcaatagaa gcacccctta cccccagacc aaaagtataa
     6361 atgaatatcg ggtctaaaat catattaagc aaagcaccta ataacatagc acacatagtt
     6421 gttttagcag ctccttcact tgtgacaata ttagtcatag tgacattgaa tatattaaaa
     6481 atagatgata cgacataaat gctagcataa gatattgcat aaggcataat actttttgta
     6541 gctcctaata attttaaaat tggattgaga aaaatcataa tcacaataat aactaatgta
     6601 cccactataa tactactgta aatagatgta cttaccactt ttcctgcttt ttcattatca
     6661 ccatggccta aaagtctggc aacatatgaa gcagctccat ttccaaacaa taatcctaat
     6721 cccacaacaa gctgacctaa tggaaatgca acggatatag ctcccatttg acttgttcca
     6781 agtccaccca caaagtatgc atcaaccaaa ttgtataatg cagttatcat catacctatc
     6841 atggtaggta atcccataga taacaatgct tttgagattg gggcagaccc taataattca
     6901 atttttttgt tactttcttt catacttaac actcctcttc tattgacatt aagtctaaat
     6961 tataatacta taaattatag taagtatgga aaatagtact ataatttata gtatttgtca
     7021 atagtaagaa aactaaagga gatgattttt atggctacaa tagatttaat tgtattggga
     7081 atagtaaagc aacaacccaa gagtgcttat gatattcaaa aggcagtgga atacaggaat
     7141 atatcacgtt gggttaaaat aagtacacca tcaatttata aaaaagtaat tcaattagaa
     7201 caaaagggat atattgaaag taaaactgtg aagaatggaa aaatgccaga aaaagcagtt
     7261 tattcattaa ctgaattagg tgagacacat tttttagatt taatgtttga tacatctagc
     7321 aaaccaataa atattctttt agattttaat gctgttatag taaatttaaa tagccttaca
     7381 ccagaaaaac agaaattatg tctcaataat attgaagaga atgttcaaga gttaaagaaa
     7441 actattgagg aaaatataag tataaagaca gaagtaccag agacagcaaa agcagtgttg
     7501 gagcaacagt tggtgctagc acaagcattg gaaacctgga taaattcttt aaaagataat
     7561 atggggaatt aggattagag aagctaactt aataatcatt aaacaaaaac cagcatattt
     7621 aataatgaga tattagaaaa aataaaacaa atgatataaa ctgaaaagta tatttaaaat
     7681 aaagctcact taattgaggg agaatgttaa actttt
//
LOCUS       DBGJFF010000160         7661 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.163, whole genome shotgun sequence.
ACCESSION   DBGJFF010000160 DBGJFF010000000
VERSION     DBGJFF010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7661
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.163"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            18..92
                     /locus_tag="ACOIAF_003426"
     tRNA            18..92
                     /locus_tag="ACOIAF_003426"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:50..52,aa:Gly,seq:gcc)
     gene            117..193
                     /locus_tag="ACOIAF_003427"
     tRNA            117..193
                     /locus_tag="ACOIAF_003427"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:151..153,aa:Arg,seq:tct)
     gene            203..278
                     /locus_tag="ACOIAF_003428"
     tRNA            203..278
                     /locus_tag="ACOIAF_003428"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:236..238,aa:Gln,seq:ttg)
     gene            287..362
                     /locus_tag="ACOIAF_003429"
     tRNA            287..362
                     /locus_tag="ACOIAF_003429"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:320..322,aa:Lys,seq:ttt)
     gene            365..453
                     /locus_tag="ACOIAF_003430"
     tRNA            365..453
                     /locus_tag="ACOIAF_003430"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:399..401,aa:Ser,seq:tga)
     gene            457..532
                     /locus_tag="ACOIAF_003431"
     tRNA            457..532
                     /locus_tag="ACOIAF_003431"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:490..492,aa:Phe,seq:gaa)
     gene            539..612
                     /locus_tag="ACOIAF_003432"
     tRNA            539..612
                     /locus_tag="ACOIAF_003432"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:573..575,aa:Met,seq:cat)
     gene            627..703
                     /locus_tag="ACOIAF_003433"
     tRNA            627..703
                     /locus_tag="ACOIAF_003433"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:661..663,aa:Ile,seq:cat)
     gene            721..797
                     /locus_tag="ACOIAF_003434"
     tRNA            721..797
                     /locus_tag="ACOIAF_003434"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:755..757,aa:His,seq:gtg)
     gene            806..881
                     /locus_tag="ACOIAF_003435"
     tRNA            806..881
                     /locus_tag="ACOIAF_003435"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:839..841,aa:Lys,seq:ttt)
     gene            889..962
                     /locus_tag="ACOIAF_003436"
     tRNA            889..962
                     /locus_tag="ACOIAF_003436"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:921..923,aa:Cys,seq:gca)
     gene            970..1046
                     /locus_tag="ACOIAF_003437"
     tRNA            970..1046
                     /locus_tag="ACOIAF_003437"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1004..1006,aa:Arg,seq:acg)
     gene            1059..1134
                     /locus_tag="ACOIAF_003438"
     tRNA            1059..1134
                     /locus_tag="ACOIAF_003438"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1092..1094,aa:Val,seq:tac)
     gene            1210..1623
                     /locus_tag="ACOIAF_003439"
     CDS             1210..1623
                     /locus_tag="ACOIAF_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670419.1"
                     /translation="MYIVIGNEIVDSEELKIIIDKNSEFKVEKDLSKSTKREDVIAYQ
                     LSIDLNYLDSLINEQCNLASLSDEEKFDEYMTLSDELALDLEELMPKYTIINARAYKL
                     DEVDGIVKIILAVAYADLGHLKLSDVVKRLSRQVD"
     gene            1932..5363
                     /locus_tag="ACOIAF_003440"
     CDS             1932..5363
                     /locus_tag="ACOIAF_003440"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HGZ2670420.1"
                     /translation="MLKKFNKILVANRGEIAIRIFRACSELGIKSVGIYSKEDKYGLF
                     RTKADESYLIGEGKGPIDAYLDMDGIIDLAKRKKVDAIHPGYGFLAENAEFARKCEEN
                     GITFIGPSSKVMNMMGDKINSKKIAKEVNVQTIPGVEKAIRSTEEAKEVANKIGYPVM
                     IKASNGGGGRGMRIVHREEDLELEYETACSESRKAFGEDIIFIEKYIADPKHIEVQIL
                     GDNYGNIVHLYERDCSVQRRHQKIIEYAPAFSLDDKVRKEICEDAVKLSKHVGYSNAG
                     TLEFLVDANGGHYFIEMNTRVQVEHTVTEMVTGIDIVQSQILIAQGYSLDSEEINIKS
                     QDDVEIRGYSIQCRITTEDPKNKFMPDTGKIQVYRTGSGFGIRLDGGNGFTGANISPH
                     YDSLLVKTISWDRTFQGAINKTIRSIKELRVRGVKTNVGFLVNVLNNPIFSNGKCSTK
                     FIDENPDLFEITESKDRGTKLLQFIGDVIVNDNACKEKPLFDALHDPRMDKDGSKSEG
                     SKILFDKLGKSAYIEKIKNDKKLLLTDTTMRDAHQSLLATRIRTYDLLKAAKPTEKYQ
                     KDLFSLEMWGGATYDVAYRFLKESPWRRLQKLREEIPSIMFQMLLRASNGVGYKNYPD
                     NVIEEFTKESARQGIDVFRIFDSLNWVENMKPSINTALETGKIVEATMCYTGDILDKT
                     KTKYNLEYYIKMAQELESLGADIIAIKDMSGLLKPYSAYTLVKELKKNVKAPIHLHTH
                     DTSGNGVATCLMASEAGVDIIDAALESMAGLTSQPSLNAIVEALKNTERDTGIDLFGY
                     DELGKYYKDLRKVYNKFESDLTNSCAEIYNFEIPGGQYTNLKPQADSLGLVNRFDEVK
                     EKYKEANEVVGDIIKVTPSSKVVGDLAIFMTKNKLDKDNIIEEGKNLSFPDSVVDYCK
                     GMIGQPEGGIPKDLQEVVLKGEEAITVRPGSLLPAEDFDEIAKYLNEKYDINANIRNV
                     ISYALYPKVYEDYIKHLQHYNDISKLESDVFFYGLNKNEECEVEIEEGKVLTIRLVEI
                     GEVKENGFRTIGFELNGMVREVEIKDKNFSGKINNVEKADMNDPLQIGASIPGKVIKI
                     MVKEEDEVKANQPLIVIEAMKMETIIVAKTDGVIKSIKVKEDDMVEDKQLLMIMK"
     gene            5605..7533
                     /locus_tag="ACOIAF_003441"
     CDS             5605..7533
                     /locus_tag="ACOIAF_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HGZ2670421.1"
                     /translation="MKVYDSKMLRNVAVLGHSGCGKTNLIETIAYTANTNKIPKLTDK
                     VNMTYSMGLIPIEYNDYKFNLLDTPGYFDFSGDVVSSLRASDAAIIVIDATAPIQVGT
                     EKSLELTESIPKIMFINKIDNEKARYKDAIAMLREKYNNKIVPMISPIYKDKNFVKLH
                     NVFENIDDLEGEFKEQAMSVKEALMELIAETDDQILDKYFNGEELTTEEIQKGIIIGI
                     QRGDIIPVICGSTINNIGTKEILDTISSYLEPIFTEESKPFRGLVFKTMVDPFVGKMS
                     YIKITEGVLSKDKDVFNINKNVKEKIANIYTLRNSELVEIEKAKAGDIVVITKVNSLK
                     TGDTISADKDAEALEKIDFPKPQIYYAVTPKNKGDEEKVASVLNKLVEEDPTLHWYRN
                     TETKQALLGGQGELHIKTIKNKMKDKFGVDVELNDLKVPYRETIKGTADVQGKHKKQS
                     GGHGQYGDVKIRFERCESDFEFTEEIFGGSVPKQYIPAVEKGLKDSMQKGILAGYPVT
                     NIKATLYDGSYHDVDSSEMAFKMAASAAFKKGMEEAHPILLEPIMKLKITVPEEYMGD
                     VMGDINKRRGKIFGMEPDDKGKQIIFAEAPQAETFKYAIDLRAMTQGRGYFEMELERY
                     GEVPSQFAEKIIGLATAK"
BASE COUNT         3043 a          940 c         1521 g         2157 t
ORIGIN      
        1 tttaatttaa taaacgagcg ggaatagttc agtggtagag cgcaaccttg ccaaggttga
       61 agtcgcgagt tcgaatctcg tttcccgctc caaattttat gtttatgttg taatgtgggt
      121 gcatagctca gctggataga gcaacgccct tctaaggcgt gtgtccgggg ttcgaatccc
      181 tgtgcgctca ccacatatat attggggatt agccaagtcg gtaaggcaca tgactttgac
      241 tcatgtatgc gtaggttcga gtcctgcatc cccagccaaa cagtatggtc tattagctca
      301 gtcggtagag cacctgactt ttaatcaggg tgtcccgcgt tcgagtcgcg gatagatcac
      361 caatggagag gtgtccgagt ggtttaagga gctggtcttg aaaaccagtg acttcgaaag
      421 gggccgtggg ttcgaatccc accctctccg ccaaatgccc agatagctca gtcggtagag
      481 caggggactg aaaatccccg tgtcggtggt tcgattccgc ctctgggcac caaaatgtgg
      541 cggtatagct tagttggcta gagcgttcgg ttcatacccg aaaggtcata ggttcgactc
      601 ctattaccgc tactgaaatt taatgtgggc cattagctca gttggttaga gcgcccggct
      661 cataaccggt aggtctgggg ttcgagtccc tgatggccca ccacattaaa ttaacttatg
      721 gtgggtatag ctcagttggt tagagcgcca gattgtggct ctggaggtcg tgagttcgac
      781 tctcattatc caccccaact tatatggtct attagctcag tcggtagagc acctgacttt
      841 taatcagggt gtcccgcgtt cgagtcgcgg atagatcacc aaaaaggcgg cgacatagcc
      901 aagtggtaag gcagtggact gcaactcctt gatccccagt tcgaatctgg gtgtcgcctc
      961 caataaaatg cgctcatagc tcaactggat agagtgtctg actacgaatc agaaggttag
     1021 gggttcgagt ccctttgggc gcaccatatg aatagcgagg gattatagct cagctgggag
     1081 agcacctgcc ttacaagcag ggggtcacag gttcgagccc tgttaatccc accagtgcaa
     1141 acctctagta aaactactag aggttttatt tttgattgaa aaagttttta tatttaggag
     1201 gtaaaaaatt tgtatatagt aataggaaat gaaatagttg atagtgaaga acttaaaata
     1261 attatagata aaaatagtga atttaaagtt gaaaaagatt taagtaaaag cacaaaaaga
     1321 gaagatgtaa tagcatatca gttaagtata gatttaaatt acctagattc tcttataaat
     1381 gagcaatgta atttagccag tctatctgat gaagagaaat ttgatgaata tatgactttg
     1441 tctgatgaac ttgcactgga tttagaagag ttaatgccaa aatatacaat aataaatgct
     1501 agagcatata aattagacga ggtagatgga attgtaaaaa taatactagc tgtagcgtat
     1561 gcagatttag ggcacttaaa actttctgat gtagtaaaga ggctatctag acaagtagat
     1621 taggagatga atatgaacta tattaaaaaa atgtataaaa tcttacgtaa agatgaaaat
     1681 aattatgtaa acatagaaaa aaacagtata aaatcaagtc aattgtagaa attttcacat
     1741 aaattcatct gaaattctat aaaaaatgcc ttaattttaa tgaggtaaat cgagtttaca
     1801 cagtctaaaa attacttata tttataaatt tatcttatta attaaggaac tttcttaaac
     1861 atcttcaaaa ataaagtatt ttagtctata atctttaata ttacacaaac aataaaggag
     1921 aagaatagag tatgcttaaa aagtttaata aaatactagt ggctaataga ggcgaaatag
     1981 ctataagaat atttagagcc tgttcagaat tggggataaa aagtgttggt atatacagta
     2041 aggaagataa gtatggattg tttagaacaa aagcggatga atcatatttg ataggagaag
     2101 gtaaagggcc aatagatgca tatttagata tggatggaat aatagaccta gcaaagagaa
     2161 aaaaagttga tgctatacat ccaggatatg gttttctagc ggagaatgca gaatttgctc
     2221 gtaaatgtga agagaatgga attactttta taggaccatc atctaaggta atgaatatga
     2281 tgggggataa aattaactct aaaaaaatcg caaaagaagt taatgttcaa actataccag
     2341 gagtagaaaa agcaataaga agtactgaag aagcaaagga agttgcaaac aaaataggat
     2401 atcctgttat gataaaagca tctaatggcg gtggcggtag aggtatgaga attgtacatc
     2461 gcgaagaaga cttagagctt gaatatgaaa ctgcttgtag tgaatcaaga aaagcttttg
     2521 gagaagatat tatatttata gaaaaatata tagctgatcc aaaacatata gaagttcaaa
     2581 tattaggtga taattatgga aatatagttc acttatatga aagagattgt tcagttcaaa
     2641 gaagacatca aaaaataata gaatatgcac cagctttttc attagatgat aaagtaagaa
     2701 aagagatatg tgaagatgca gttaagttat caaaacatgt gggttattct aatgcgggaa
     2761 cattagaatt tttagtagat gcaaatggtg ggcattattt tatagagatg aacacaagag
     2821 tacaggttga acatactgta acagagatgg ttacaggaat agatatagtt caaagtcaaa
     2881 tactaatagc ccaaggttac agcttagata gtgaagaaat taatataaaa tcacaagatg
     2941 atgtggaaat aagaggttac tctatccaat gtagaataac tacagaagac cctaagaata
     3001 aattcatgcc tgatacaggt aaaattcaag tttatagaac tggttcagga tttggtataa
     3061 gactagatgg aggaaatgga tttacagggg caaacataag tcctcactat gatagtttat
     3121 tggttaagac aatatcttgg gatagaacat tccaaggagc aataaataaa actataagat
     3181 ctataaaaga acttagagtt agaggtgtta aaactaatgt aggattccta gttaatgttt
     3241 taaataatcc tatattctca aatggtaaat gtagtactaa gtttatagat gaaaatccag
     3301 atttatttga aataacagaa agtaaagaca gaggaacaaa attacttcaa tttataggag
     3361 atgtaattgt aaatgataat gcttgtaaag aaaaaccatt atttgatgca ttacatgacc
     3421 caagaatgga taaggatggt tcaaaatcag aaggaagtaa aatacttttt gataagttag
     3481 gtaaatctgc atatatagaa aagataaaaa atgataagaa attactatta acagatacaa
     3541 caatgagaga tgcacatcaa tcattattag caacaagaat aagaacatat gacttattaa
     3601 aagctgctaa gccaacagaa aaatatcaaa aggatttatt ctcacttgaa atgtggggag
     3661 gagctactta tgatgttgca tatagatttt taaaagaatc tccttggcgt agattacaaa
     3721 agctaagaga agaaatacca agcataatgt tccaaatgtt gcttagagca tcaaatggag
     3781 ttggatataa aaactatcca gataatgtaa tagaagaatt tacaaaagaa tctgctagac
     3841 aaggtataga tgtatttaga atatttgatt cattaaactg ggttgaaaat atgaaaccat
     3901 ctattaatac agctttagaa actggaaaaa ttgttgaagc aactatgtgt tatacaggtg
     3961 atatactaga taagactaaa actaaataca atctagaata ttatattaaa atggcacaag
     4021 aattagaaag cttgggagca gacataatag caataaaaga tatgtcaggt cttcttaaac
     4081 catattcagc ttacacatta gttaaagagt taaagaaaaa tgtaaaagca ccaatacatt
     4141 tacacactca tgatacaagt ggaaatggtg tagcaacttg tttaatggcg tcagaagctg
     4201 gagtagacat aattgatgct gcattagaat ctatggcagg acttacaagt cagccatcac
     4261 ttaatgctat agtagaggca cttaagaata cagagagaga tacaggaata gatttatttg
     4321 gatatgatga gttaggaaaa tattataagg atttaagaaa agtttataat aaatttgaaa
     4381 gtgatttaac taattcttgt gcagaaatat ataattttga aataccagga ggccaatata
     4441 caaacttaaa acctcaagct gatagtttag gattagttaa tagatttgat gaagttaaag
     4501 aaaaatataa agaagctaat gaagttgtag gagatataat aaaagtaact ccatcttcaa
     4561 aagttgtagg agacttagct atctttatga ctaaaaataa gttagataaa gacaatataa
     4621 tagaagaagg taaaaattta tcattcccag attctgttgt ggattactgt aaaggtatga
     4681 ttggtcaacc agaaggtgga ataccaaaag atttacaaga ggtagttcta aaaggtgaag
     4741 aagctataac agtaagacca ggttcattat taccagcaga agattttgat gaaatagcta
     4801 aatatctaaa tgaaaagtat gatataaatg ctaatataag aaatgttata agttatgctt
     4861 tatatccaaa agtatatgaa gattatatca aacatctaca acattataat gatatatcta
     4921 aacttgaaag tgatgtattc ttctatggat taaataaaaa tgaagaatgt gaagtagaaa
     4981 tagaagaagg taaggtatta acaataagat tagttgaaat tggtgaagtt aaggaaaatg
     5041 gatttagaac tataggattt gagttaaatg gaatggttag agaagtagaa ataaaagata
     5101 agaatttctc tggaaaaatt aataatgttg aaaaagctga tatgaatgac cctcttcaaa
     5161 ttggagcaag cataccagga aaagttataa aaataatggt taaagaagaa gatgaagtaa
     5221 aagcaaatca accattaatc gttatagagg ctatgaagat ggaaactata atagttgcta
     5281 agactgatgg agtaattaag tcaatcaaag taaaagaaga tgatatggtt gaagataaac
     5341 aattattaat gataatgaag taatttaatt aaaataaatt aaaaagcact ctgaaatgtt
     5401 taataaaaaa tgttttagag tgtttttatt tattttaatt aaatatttgt taaaaaaaag
     5461 aggacaatta cctccagatg tagaaataaa gctagtaagg aaaatgttat ctaaaaattc
     5521 taaatatagt aaatattttc cataatattt cttaggaaat aacagcaaag ataaaataca
     5581 taaatttaaa aggtgggtgc ttatatgaag gtttatgata gtaaaatgtt gagaaatgta
     5641 gcggtattag gacatagcgg atgtggaaaa acaaatctaa tcgaaactat agcttacaca
     5701 gcaaacacta ataagatacc taaattaaca gacaaggtaa atatgactta tagcatggga
     5761 ttaataccta tagagtataa tgattataag tttaacttat tggatacccc aggatatttt
     5821 gactttagcg gagacgttgt gtcatctctt agagcaagtg atgcagcaat aatagtaata
     5881 gatgcaacag cgcctattca agttggtaca gagaaatcat tagaactaac tgagagtatt
     5941 cctaagataa tgtttataaa taaaatagat aatgaaaagg caagatacaa agatgcaata
     6001 gcaatgttaa gagaaaaata caataataag atagttccta tgataagtcc tatatacaaa
     6061 gataaaaact ttgtaaagtt acataatgtt tttgaaaaca tagatgattt agaaggtgaa
     6121 tttaaggaac aagctatgag tgttaaagaa gcattaatgg aacttatagc agaaactgat
     6181 gaccaaatat tagataaata ttttaatgga gaagaactaa ctacagaaga gatacaaaaa
     6241 ggtattatta taggtataca aagaggtgat attatacctg taatatgtgg ttcaactata
     6301 aataatatag gcacaaagga aatattagat acaatatcaa gttatttaga acctatcttt
     6361 acagaggaaa gcaaaccatt taggggatta gtatttaaaa ctatggttga cccatttgtt
     6421 ggaaagatgt catatataaa aattactgag ggtgtacttt ctaaggataa agatgttttt
     6481 aatataaata aaaatgtaaa agaaaaaata gctaatatat atacattgag aaatagtgaa
     6541 ttagtagaaa tagaaaaagc taaagctggt gatatagtag ttataactaa agttaattct
     6601 ctaaaaacag gagacactat atctgcagat aaagatgcag aagctttaga aaagatagat
     6661 tttcctaaac cacaaatata ttatgcagta actcctaaga ataaaggtga tgaagaaaaa
     6721 gtagcatctg tattaaataa actagtagaa gaggacccta cattacattg gtatagaaat
     6781 acagagacaa aacaagcact tttaggaggt caaggagaac ttcatataaa aacaattaaa
     6841 aataaaatga aagataagtt tggtgttgat gttgaattga atgaccttaa agttccttat
     6901 agagaaacta taaaaggaac agctgatgta caaggaaaac ataaaaagca atcaggtggt
     6961 catggtcaat atggagacgt aaaaataaga tttgaaagat gtgaatctga ttttgaattt
     7021 acagaagaaa tatttggtgg ttctgtacca aaacaatata taccagcagt tgaaaaaggt
     7081 ttaaaagact ctatgcaaaa aggtatatta gcaggatatc ctgttacaaa cataaaagct
     7141 acgctttatg atggttcata ccatgatgta gactcttctg aaatggcctt taagatggca
     7201 gcaagtgcag cctttaaaaa aggtatggaa gaagcacacc caatactact agaacctata
     7261 atgaaactta agataactgt tccagaagaa tatatgggag atgtcatggg tgatataaat
     7321 aagagaagag gaaaaatatt tggaatggaa ccagatgaca aagggaaaca aataatattt
     7381 gcagaagctc cacaagcaga gacatttaaa tatgcaatag atttaagagc aatgacacaa
     7441 ggtagaggat actttgaaat ggaattagag agatatggag aggttccatc acagtttgca
     7501 gaaaaaataa ttgggttagc tactgcaaag taatgaacaa aataaataga aaaataatta
     7561 atatatatta ttaaaaatat ttttagggtt ttaactaggt ataggtttat gttaggtaaa
     7621 cttataccta gtttttatac atttttgatg aataaaaaaa t
//
LOCUS       DBGJFF010000161         7603 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.164, whole genome shotgun sequence.
ACCESSION   DBGJFF010000161 DBGJFF010000000
VERSION     DBGJFF010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7603
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.164"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            220..1413
                     /gene="nifV"
                     /locus_tag="ACOIAF_003442"
     CDS             220..1413
                     /gene="nifV"
                     /locus_tag="ACOIAF_003442"
                     /EC_number="2.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004410 - homocitrate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HGZ2670422.1"
                     /translation="MCVISKDRAKEIKIVDTTLRDGEQTAGVVFANREKIMIAEMLSD
                     LGVDQIEVGIPTMGGDEKNVIKHICSRNLKSDIMAWNRAVIKDVEESISCGVDAVAIS
                     ISVSDIHIENKLRTSRGWVLENMAKTVEFAKKNGLYVSVNGEDASRADIDFLTEFINV
                     GKQAGADRFRYCDTVGVMNPFSIKNAIETLYERTNFDIEMHTHNDFGMATANALAGIA
                     AGANYVGVTVNGLGERAGNAALEEVLMALKCVYKCDLNNIDTRKFRGICEYVAQASGR
                     ILPTWKPVVGDNMFIHESGIHADGALKDPHNYEPFDPSEVNLERKIVIGKHSGRAAVV
                     NKLSEYEMYISPENATKLLNAIRATSIRLKRSLMDKEILQLYCDILAHEKGTTEEEAV
                     RGSYI"
     gene            1438..3363
                     /locus_tag="ACOIAF_003443"
     CDS             1438..3363
                     /locus_tag="ACOIAF_003443"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HGZ2670423.1"
                     /translation="MGDNIVYKIIKKHIVDGEAVAGSSIGIKIDQTLTQDSTGTMTYL
                     QLEAMGIDKVKTKRSVAFVDHNMLQQGFENADDHKYIQTVADKYGVYFSKPGNGICHQ
                     VFLERFSTPGDTLLGSDSHTPTAGGVGMMAIGAGGLDVALAMAGGAYYIKAPKVCKVN
                     LVGKLNNMVSSKDIILEVLRKQTVKGGVGKVYEYGGEGVKSLSVPQRATITNMGAELG
                     ATTSIFPSDEKTLEFFKSQGREDAWVELKPDADAVYDEEITINLDELKPLAAKPHSPD
                     NVDEVENIGKIKIDQVAIGSCTNSSYEDLMKVAQILKGNKVHKDVSLVIAPGSRQVME
                     MIARNGALADIISAGARILENSCGPCIGMGQSPGTDSVSLRTFNRNFYGRSGTLSAQV
                     YLVSPEVAAVSAIKGVLTDPREFDIKFTNLDVNEFLIDDSMIIKPADVGSDVEVVRGP
                     NIKPFPLNTELSQSIGGKVILKTEDNITTDHIMPSNAKLLPFRSNIPYLANYCFNTVD
                     TEFPQRAKDNNGGFIVGGDNYGQGSSREHAALAPLYLGVKGVIVKSFARIHKANLINS
                     GIIPMEFCDEKDYENISLLDNLEIPNILDNLGSGILEVKNTTKGTSFKVKVELSAKEV
                     DVLKAGGKLNYTKNQAN"
     gene            3385..4380
                     /locus_tag="ACOIAF_003444"
     CDS             3385..4380
                     /locus_tag="ACOIAF_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HGZ2670424.1"
                     /translation="MYKVTLIPGDGIGPEVAKAMKKVVEATGVEIEWEEVNAGEAVIE
                     EYGTPLPEYIIDSIKKNKIAIKGPITTPVGKGFRSVNVTLRQALDLYVNLRPIKSFKG
                     IKSRYEDVDLVVVRENTEDLYAGIEHKIGDYAAESIKIITRSASERIVDFACNYVKDN
                     KRKKVTAIHKANIMKMSDGLFLDVFREVASKHGVEYDDLIVDAAAMNLVLNPENYDVM
                     VMPNLYGDILSDLGAGLVGGLGIIPSANIGKDCAIFEAVHGSAPQIAGQNKANPTALI
                     QSSVMMLRYLGEYENAQKIETALEKVFLEGSKLTVDLGGSASTTEFADEVCKYIV"
     gene            4730..5179
                     /locus_tag="ACOIAF_003445"
     CDS             4730..5179
                     /locus_tag="ACOIAF_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HGZ2670425.1"
                     /translation="MIKTLDSNILREVGTLSRAVNSINDIKYKELKLQKGQFTFLTRI
                     CENPGINLVELSNMLKVDKATTTKAIQKLIKAGYVDKKQDKFDKRGYNLTPTDKSLEV
                     YELIIEEENRSIEICFDNFTDEEKQVVTKLLEKMSKNVENEWFKVKR"
     gene            5213..5707
                     /locus_tag="ACOIAF_003446"
     CDS             5213..5707
                     /locus_tag="ACOIAF_003446"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGZ2670426.1"
                     /translation="MIRKANMNDLESIMKIIKSTVEEMKTYNNTQWDENYPLEKDFVS
                     DIKKQDLYIYEVDGEVAGFICLNYEEPEEYLGLNWSSNKKAMVIHRMAVNPNFRKTGI
                     ASKLVDFAEKLAVENNVSYLKSDTYSINSKMNLLLTKCGFIKIGEMSFLGKEKSFYCY
                     DKIL"
     gene            5804..6325
                     /locus_tag="ACOIAF_003447"
     CDS             5804..6325
                     /locus_tag="ACOIAF_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HGZ2670427.1"
                     /translation="MIGFLKKRRSIRKYKDVEVEKEKLDKILKAALLAPSSKGLRTWE
                     FIVVDDKEKLINLSQCRTKGGGFFLKNAPLAIVIIADKEKNDVWIEDASIAASYIQLQ
                     AHELGLGSCWIQVRNRMHDDNIEADKYIREELKVPSKYSVECIISIGYPDEEKKAYSD
                     SDLDYKKVHFNHF"
     gene            6514..6825
                     /locus_tag="ACOIAF_003448"
     CDS             6514..6825
                     /locus_tag="ACOIAF_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGZ2670428.1"
                     /translation="MGIFKTKMDEDWKVNYIKEFNEMRDSYESKLQKKQFEVDSLKSE
                     LDRLRSYKNSLKPKEKQITDDDINNIKSLRRDGLSYKEISNQTSWSKATVSRVLNGLY
                     D"
     gene            6853..7539
                     /locus_tag="ACOIAF_003449"
     CDS             6853..7539
                     /locus_tag="ACOIAF_003449"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HGZ2670429.1"
                     /translation="MKYSYKKCIIDSILLMFIVQILRMILNYVLLSQFEFTLENFNII
                     NLISFTLVGLSLILFLKDNSLYNKVRNRKITEAFEENKNNILIEKCKLILFVVVLSLA
                     IIVTYCTKGYVLFNVTMMTLSVLIVPIFEELFFREYIWNYLSNFIKSKSKIICITSIL
                     SGIYNIGYIDVIRNYVILYNNSSYTFEVIISKIMIGTAFGIVLGLVKYRFRDVGFCIL
                     LRSLFAIFIR"
BASE COUNT         3036 a          762 c         1487 g         2318 t
ORIGIN      
        1 actttcattt ttaagaagtt ttcacaaaaa agaaaaaata tagtgcaata aatgatacaa
       61 agtaatataa tagattacaa gatatgaaaa gctaaaaaaa taaattgcaa atgaaattaa
      121 aatgaataaa aaataaaaaa ataatcataa gttagaggta tcaatgaaaa ataagactta
      181 atgttttaaa attaaaagta tactaagggg ggcattaata tgtgcgttat atcaaaagat
      241 agagcaaagg aaataaaaat agtagatact actttaagag atggagagca aacagctggg
      301 gttgtatttg caaatagaga aaaaataatg attgctgaaa tgttaagtga cctaggtgtt
      361 gaccaaattg aagttggtat acctactatg ggtggagatg aaaaaaatgt aataaagcac
      421 atatgtagta gaaatttaaa atcagatata atggcttgga atagagcagt aataaaagat
      481 gttgaagaat ctatttcttg tggtgttgat gcagtagcta tatcaatatc agtatctgac
      541 attcatatag aaaataaatt gagaacttct agaggctggg ttttagaaaa tatggctaaa
      601 acagttgaat ttgcaaagaa aaatggatta tatgtttcag taaatggaga agatgcttca
      661 agagcagata tagacttctt aactgaattt ataaatgttg gaaaacaagc aggggcagat
      721 agatttagat actgtgatac agtaggagtt atgaatcctt tttctataaa gaatgcaata
      781 gaaactcttt atgaaagaac taattttgat atagaaatgc atacacataa tgactttggg
      841 atggcaactg caaatgcact tgcaggaata gcagcaggag caaattatgt tggtgttaca
      901 gtaaatggat taggtgaaag agcaggaaat gcagcattag aagaagtttt aatggcactt
      961 aagtgtgttt ataaatgtga tttaaataat atagatacta gaaaatttag aggaatatgt
     1021 gagtatgtag cacaggcatc aggaagaata cttcctacat ggaaaccagt agttggggat
     1081 aatatgttta tacatgaatc tggtatacat gcagatggag ctttaaaaga cccacataac
     1141 tatgaaccat ttgacccaag tgaagtaaat cttgaaagaa aaatagtaat aggtaaacat
     1201 tcaggaagag ctgcagttgt aaataagttg agtgaatatg aaatgtatat ttctccagaa
     1261 aacgctacca agctattaaa tgctataaga gctacatcta taagattaaa aagaagttta
     1321 atggataaag aaattcttca attatattgt gatatactag cacatgagaa aggcacaaca
     1381 gaagaagaag cagttagagg ttcatatatt taagatataa ttgtttagga ggaaaatatg
     1441 ggagataata tagtttataa aataattaaa aaacatatag tagatggaga agctgttgct
     1501 ggaagttcaa ttggtataaa aatagaccaa actttaacac aagattctac tggaacaatg
     1561 acatatcttc aactagaagc tatgggaata gataaagtaa agactaaaag aagtgtggca
     1621 tttgtagacc ataatatgtt acaacaagga tttgaaaatg cagacgacca taagtatata
     1681 caaacagtgg cagataaata tggagtttat ttctctaagc caggaaatgg tatttgtcat
     1741 caagtatttt tagaaagatt ttcaacacca ggagatacac ttttaggttc tgatagccac
     1801 acacctactg ctggaggtgt gggcatgatg gctattggag ctggtgggtt agatgttgct
     1861 ttagccatgg caggaggagc atactatata aaagcaccaa aagtctgtaa ggtgaatcta
     1921 gttggaaaat taaataatat ggtatcatca aaagatataa tattagaagt gttaagaaaa
     1981 caaactgtaa aaggtggagt aggtaaagtt tatgaatatg gtggagaagg tgtcaaaagc
     2041 ctatctgttc cacaaagagc tacaataaca aatatggggg ctgaacttgg agcaactact
     2101 tcaatcttcc caagtgatga aaaaacacta gaatttttta agagtcaagg aagagaagac
     2161 gcatgggtag agctaaaacc agatgcagat gcagtttatg atgaagagat tactataaac
     2221 ttggatgaat taaaaccatt agctgcaaaa cctcatagcc cagacaatgt agatgaagtt
     2281 gaaaatatag gcaagattaa aatagaccaa gtggcaatag gaagttgtac aaattcttct
     2341 tatgaagact taatgaaagt agcacaaatc ctgaaaggaa ataaagtaca taaggatgtg
     2401 agtttggtaa tagcaccagg ttcaagacaa gttatggaga tgattgctag aaatggggct
     2461 ttggcagata taataagtgc tggagctaga atactagaaa attcttgtgg tccatgtatt
     2521 ggtatgggtc aatctccagg tactgactct gtgtctttaa gaactttcaa tagaaatttc
     2581 tatggtagaa gtgggacttt gtcagcacaa gtatatttag taagtccaga agtggcagca
     2641 gtaagtgcaa tcaagggagt gcttacagac ccaagagaat ttgatattaa atttacaaat
     2701 ttagatgtaa atgagttttt gatagatgat agcatgataa ttaaacctgc tgatgtagga
     2761 agtgatgtag aagtagttag aggacctaat ataaaacctt tccctcttaa tacagagcta
     2821 agtcagtcta taggtggaaa agttatatta aagacagaag ataatataac tactgaccat
     2881 ataatgccat caaatgctaa gttacttcct tttagaagta atataccgta tcttgcaaac
     2941 tattgtttca atactgtaga tacagaattt cctcagagag caaaagacaa taatggtgga
     3001 tttatagttg gtggagataa ttatggtcaa ggttcaagta gagaacatgc agctttagca
     3061 ccattatatc taggtgttaa aggggtaata gttaagagct ttgcaagaat ccacaaggct
     3121 aacttgataa atagtggtat aattccaatg gagttttgtg atgagaaaga ttatgaaaat
     3181 atatctcttt tagataatct ggagatacca aatattttag acaatctagg tagtggaatt
     3241 ttagaagtta agaatacaac taaaggaact tcttttaagg tcaaagtaga attgtcagca
     3301 aaagaagttg atgtattaaa agctggtgga aaattaaact acactaaaaa tcaagctaat
     3361 taatatgaaa agagggaata ttgaatgtat aaagtgacac ttataccagg agatggaata
     3421 ggaccagaag ttgcgaaagc tatgaaaaag gtagtagaag ctactggagt agaaatagaa
     3481 tgggaagaag ttaatgcagg agaggcagtt attgaagagt atggtactcc acttcctgaa
     3541 tatattatag atagtataaa aaagaacaaa atagctataa aaggcccaat tactactcct
     3601 gtaggtaaag gatttaggag tgtaaatgta actttaagac aggcattaga tttgtatgtc
     3661 aatttaagac ctattaaaag ttttaagggg ataaaatcaa gatatgaaga tgttgattta
     3721 gtggttgtaa gagaaaatac agaagattta tatgctggaa tagaacataa aataggtgat
     3781 tatgcagctg aaagtataaa gataataaca agaagtgcaa gtgaaagaat cgttgatttt
     3841 gcatgtaact atgttaaaga taataagaga aaaaaagtta cagctataca taaggcaaat
     3901 ataatgaaaa tgtctgatgg attgttttta gatgtgttta gagaagttgc atcaaaacac
     3961 ggtgtagaat atgatgattt gattgtagat gcagcagcta tgaacttagt tttaaatcca
     4021 gaaaattatg atgtaatggt aatgccaaat ctttatggag atatactatc agatttaggt
     4081 gctgggcttg ttggaggttt aggtataatt ccaagtgcaa atattggtaa agattgcgcg
     4141 atatttgaag ctgttcatgg aagtgctcca caaatagcag gtcaaaataa ggctaatcca
     4201 acagcactta ttcaatcatc agtaatgatg cttagatact taggtgaata tgaaaatgca
     4261 caaaaaatag aaacagcttt agaaaaagta tttcttgaag gaagtaaatt gactgtagat
     4321 ttgggtggct cagcttctac tacagagttt gcggatgagg tgtgtaagta tattgtatag
     4381 attttattta gggaaaaagt aaaatataga ttgaaggaaa gaggctttct caaaatagag
     4441 ataagccttt tttattggaa tgataaaata tatttgattt tatacaaaag agagttcctc
     4501 ataaacgaaa aagttaattt tgggatatcc attttaattt taaaattaat tgattaatat
     4561 gcaaagaaag attaaataaa tttattattt ttaattgaac tttcaaccaa aatatggtaa
     4621 aatttaaaaa agttgaactt tcaactaaaa atgaatatat tgattatgtg aaattctata
     4681 aaacagaaac tatttgactt tagccatagt agtttcagtg gaggtgtgtt tgattaagac
     4741 tttagacagt aatatattaa gagaagtagg tactttatct agagcagtaa attcaataaa
     4801 tgatattaag tacaaggaat taaagttaca aaaaggacaa tttacatttt tgacaagaat
     4861 ctgtgaaaat cctggtataa atcttgtgga attatcaaat atgttaaagg tagacaaggc
     4921 tacaacaacc aaggctatac aaaaattaat aaaggcagga tatgtagata aaaagcagga
     4981 taagtttgat aaaagaggat ataatttaac tccaactgat aaatctttgg aagtttatga
     5041 gttgataatt gaagaagaga atagaagtat agagatatgt tttgataatt ttacagatga
     5101 agaaaaacaa gtggtaacta agttattaga aaaaatgagc aaaaacgtag aaaatgaatg
     5161 gtttaaagta aagagatagt aaataaatac attaacttaa ggagatattt ttatgattag
     5221 aaaagcgaat atgaatgatt tagaaagcat tatgaaaatt ataaaatcta cagtggaaga
     5281 aatgaagact tataataata cacaatggga tgaaaactat ccattagaaa aagactttgt
     5341 ttcagatatt aagaaacaag atttatatat ttatgaagtg gatggagaag tagcagggtt
     5401 tatatgtctt aattatgaag agccagaaga atatttaggc ttaaattggt catctaataa
     5461 aaaagcaatg gtaatacata gaatggctgt aaatccaaac tttagaaaga caggaatagc
     5521 atctaagtta gttgattttg ctgaaaaatt agctgtagaa aacaatgtat catacttaaa
     5581 gagcgataca tattcaataa actcaaaaat gaatttatta cttacaaagt gtggttttat
     5641 aaaaatcgga gaaatgagtt ttttaggaaa ggagaaatct ttctactgtt atgataaaat
     5701 attataaaga gcatttataa tacaaggggt aaataataga ttttcagcat tagtaagggc
     5761 ataatataac ctagtatatg ctattatgaa agggatgatt tatatgattg gatttctaaa
     5821 gaaaagaaga agtattagaa aatacaagga tgttgaagta gaaaaagaaa aattagataa
     5881 aatattaaaa gctgctcttt tagctccatc atcaaagggg cttagaacat gggaatttat
     5941 agtggtagat gataaagaaa agctaattaa tttatctcaa tgtagaacta aaggtggtgg
     6001 atttttctta aaaaatgcac ctttagctat tgtaattatt gcagataaag aaaaaaatga
     6061 tgtttggata gaggatgcaa gtatagctgc ttcatacata caacttcaag ctcatgaact
     6121 aggtttaggt tcttgttgga tacaagtaag aaatagaatg catgatgata atatagaagc
     6181 tgataagtac attagagaag aattaaaagt acctagcaag tacagtgtag aatgtataat
     6241 ttctatagga tatcctgatg aagaaaagaa agcatacagt gactctgatt tagactataa
     6301 aaaggttcat tttaaccact tttagtatat aatatgttgt attaagacat tatatttttt
     6361 tctattgtta atatatactt aaaattgtaa aaatatatct gaattttact aacttatcta
     6421 ggggttatgt acaaggattt taaagataaa tatagaaatt aatgtttata atcgatacat
     6481 aacttgagta acaaaattta aggaggttaa agtatgggta tatttaaaac aaaaatggat
     6541 gaggactgga aagtaaacta cattaaagag tttaatgaaa tgagagattc ttatgagagt
     6601 aaacttcaaa aaaaacagtt tgaggtagat agtttgaaat cagaattgga tagactaaga
     6661 agctataaaa atagccttaa gccaaaagaa aaacaaatta ctgatgatga tataaataat
     6721 ataaagagtt taagaagaga tggcttaagc tataaagaaa tttcaaatca aacaagttgg
     6781 agcaaagcaa ctgtaagtag ggtattaaat ggactttacg attagcatat acatggggtg
     6841 atttaaattg ggatgaagta tagttacaaa aaatgcataa tagattctat tttattgatg
     6901 tttatagttc aaatattaag gatgattttg aattatgtac ttctaagtca atttgaattt
     6961 actcttgaaa attttaatat tataaactta atttctttta cattagttgg tttatcgtta
     7021 attttatttt taaaagataa ttctttgtat aacaaggtta gaaatagaaa aatcacagaa
     7081 gcttttgaag aaaataaaaa caatatactt attgaaaagt gtaagctgat actttttgtt
     7141 gtggttttat ctttagcaat aattgtaact tattgtacta agggatatgt actttttaat
     7201 gtaactatga tgactttgtc cgtattgata gtcccaatat ttgaagagtt attttttaga
     7261 gaatatatat ggaactatct aagtaatttt ataaagtcaa agagtaaaat tatctgtata
     7321 acatctatac tatctggaat ttataatata ggatacatag atgttataag aaattatgta
     7381 atattgtata acaattcgag ctacaccttt gaagttatta tttcaaaaat tatgataggt
     7441 actgcttttg gtattgtact tggattagtt aagtataggt ttagagatgt agggttttgt
     7501 atattattga ggagcttatt tgctatattt attaggtaga tagtataatc ggtttataag
     7561 gttgttttaa atttaatttt aaatcaacct tattttattt gtg
//
LOCUS       DBGJFF010000162         7492 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.166, whole genome shotgun sequence.
ACCESSION   DBGJFF010000162 DBGJFF010000000
VERSION     DBGJFF010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7492
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.166"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            122..1099
                     /locus_tag="ACOIAF_003450"
     CDS             122..1099
                     /locus_tag="ACOIAF_003450"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HGZ2670430.1"
                     /translation="MRDKIIEIILNNKSEFISGEELSKQLGVSRAAIWKHMKALKEEG
                     YNIESVNKKGYRLAENPTDLLSPQNIYYKLDTEFIGKNIEHLDTIDSTNEHAKRIAVN
                     SVDGTVILSEHQDMGKGRLGRRWDSKPHEGIWMSIILKPDMEPFKAPFITLIAGASVT
                     KALNNLGANTLIKWPNDVILNGKKICGILTELSAEIERTNYVVLGIGINVKIMDFEQE
                     IADKATSLYKEGYMLSRVDIVRNVLTEFEKLYLDYTVNNSKEKTLKICRDYSAIIDKN
                     IYVLKGDNRELVKCLDINEEGNLLVQRSDGSIKEIISGEVSIRGEKGYV"
     gene            1092..2060
                     /locus_tag="ACOIAF_003451"
     CDS             1092..2060
                     /locus_tag="ACOIAF_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HGZ2670431.1"
                     /translation="MYNNILDIEGLKVGQVEDRNGLTGCTVVICEEGATCGVDVRGSA
                     PGTRETDLLDPVNMVQKVHAVVLAGGSAYGLESTCGVSRYLEEKGIGFDVGVGKVPIV
                     VGAVLFDLGVGSFKCRPDLKMGYRACEVANTDILNQGNYGAGCGATVGKIRGQEYAMK
                     GGIGSYSIKLDNGLIVSALIAVNALGDVYENGKVIAGALNDKKTETIDTYKLMKQGES
                     KGGFSVDNTTIGIIATNAKLTKAGCKKISQMGHDGYAKSIFPIHTPHDGDTIFTISTG
                     EIEADITLVGSLAVEVVEKSIINAVKKADSVENIMAYKDIVKKREI"
     gene            2244..2834
                     /locus_tag="ACOIAF_003452"
     CDS             2244..2834
                     /locus_tag="ACOIAF_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HGZ2670432.1"
                     /translation="MMNTSASVRKVNVRKMTIIGVLSAISIMLSMTPLGFIPVGPTKA
                     TIMHIPVIIGAIMEGPIVGAAIGFIFGISSLLNAITNPTVTSFVFINPLVSILPRVMI
                     GVLAYYVYQLIIKATNKVYISGLITGAIGSLLNTAGVLGMIYVLYADKYLHAMGKSGS
                     AGKVIMALAAANGVPEAIVGALVVAAVATVLKKSKK"
     gene            2853..3623
                     /locus_tag="ACOIAF_003453"
     CDS             2853..3623
                     /locus_tag="ACOIAF_003453"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HGZ2670433.1"
                     /translation="MLLVFDVGNTNMVLGIYKGDKLVNYWRIKTDREKTSDEYGILIS
                     NLFDYDNVNISDIDDVIISSVVPNVMHSLENFCIKYCKKQPLIVGPGIKTGLNIKYDN
                     PKQVGADRIVNAVAGIEKYGAPSILVDFGTATTFCAISEKGEYLGGTIAPGIKISSEA
                     LFQSASKLPRVELAKPGMTICKSTVSAMQSGIIYGYVGLVDKIISMMKKELNCDDVKV
                     IATGGLAKLIASETKSIDYVDGFLTLEGLRIIYEKNQE"
     gene            3760..4728
                     /gene="dusB"
                     /locus_tag="ACOIAF_003454"
     CDS             3760..4728
                     /gene="dusB"
                     /locus_tag="ACOIAF_003454"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HGZ2670434.1"
                     /translation="MKIGNLELKNKVFLSPMAGVTDLPFRLICKEQGCGLLYTEMING
                     KALCYDDENTKKMLKIEEEEHPVAVQIFGSEPEFMGRAAEIMNDYSNEILDINMGCPA
                     PKVVKNGDGSALMKNPKLAEEVLRAVVKNSKKPVTLKIRKGWDDNSVNAVEIAKIAED
                     CGISALAIHGRTREQFYTGKADWDIIAEIKKNLSIPVIGNGDVFTIEDSINMLDKTGC
                     DAIMIGRGAQGNPWIFKRINHYMNTGEILPEPTLNEKISTAIKHLKLAVEEHGEYVAV
                     REMRKHIAWYLKGLRNSAKLRDEINKIEDYQEVVSKLEYYMEDSLT"
     gene            4837..5316
                     /gene="greA"
                     /locus_tag="ACOIAF_003455"
     CDS             4837..5316
                     /gene="greA"
                     /locus_tag="ACOIAF_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HGZ2670435.1"
                     /translation="MEENKEFLLTQEGYDKLEEELENLKVVKRKEVAERIKVAISFGD
                     LSENAEYDEAKKEQAQVEERILKLENMVRKAVIIDESKIDLNVVTIGSIVKVKDLEFD
                     EEVEYTIVGSTEADPYDGKISNESPVGKALLGRAAKEVVEVQVPDGVAKFEILEIRR"
     gene            5331..6860
                     /gene="lysS"
                     /locus_tag="ACOIAF_003456"
     CDS             5331..6860
                     /gene="lysS"
                     /locus_tag="ACOIAF_003456"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HGZ2670436.1"
                     /translation="MKNNQQSNEEAQIQEDLSEVLQVRRDKLKKLQESGRDPFKESRY
                     DRTHYSMDIKDNFDSLEGKTTKIAGRIMSKRIQGKAGFIDIQDQEGRIQSYVRLDAIG
                     EEEYSVFSTYDIGDIVGIEGEIFKTKKGEISVKAKSVVLLCKSLQVLPEKYHGLKDQE
                     LRYRQRYVDLIVNPEVKNAFLIRTKALKALRAYLDDRGFLEVETPILNTIAGGANARP
                     FITNHNTLHIPMYLRIANELYLKRLIVGGFDKVYEMGRMFRNEGMDLKHNPEYTAIEL
                     YQAYADYTDMMEITENVIAHMAEVATGSMIVNYQGTEINFTPPWKRMSMEDCVKEYSG
                     VDFSTINTDEEALEVAREKGIEIKPGMRRGEVINAFFEEFGEDKLIQPTFITHHPVEV
                     SPLSKRNVEDPRRTDRFEAFANKWELANAFSELNDPIDQKGRFIDQLRKRELGDDEAF
                     EMDEDFLKALEVGLPPTGGLGIGIDRVIMLLTNSPSIRDVLLFPTMKPIDNNPNKEEE
                     N"
     gene            7169..7363
                     /locus_tag="ACOIAF_003457"
     CDS             7169..7363
                     /locus_tag="ACOIAF_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670437.1"
                     /translation="MFQVINFLCLFCVGFLVLDVMIYFCKDAMDIFEGTTNVNKVKSI
                     NVWADRVRANSYSYENKIAK"
BASE COUNT         2993 a          755 c         1511 g         2233 t
ORIGIN      
        1 caaccattat aataattaaa aaggaaattt aaaattaata gtattaaaag tatattaata
       61 atgtataatt atttattaga agaaaataag tcatgaaatt atgtcatatt agaggtgaaa
      121 tatgcgagat aaaataatag agattatatt aaataataaa tctgaattta tttcaggaga
      181 agaattatca aaacaattag gggtgtcacg tgcagctata tggaaacata tgaaagcctt
      241 aaaagaagag ggatataata tagaatctgt aaataaaaaa gggtatcgat tagcggaaaa
      301 tccaactgat ttattaagcc cacagaatat ttactataaa ttagatacag agtttattgg
      361 aaaaaatatt gaacatcttg acactataga ttcaacaaat gaacatgcta aaaggatagc
      421 tgtaaatagt gtagatggga ctgtgatttt aagtgaacat caagatatgg ggaaaggacg
      481 attgggaaga agatgggatt caaaacctca tgaaggaatc tggatgagta ttatattaaa
      541 accagatatg gaaccattca aagctccatt tataacttta atagcaggag caagtgtaac
      601 gaaagcttta aacaatctag gagcaaatac attaataaaa tggccaaatg atgttatttt
      661 aaatggaaaa aaaatatgtg gtatattaac agagttatct gctgaaattg aaagaacaaa
      721 ttatgttgtt cttggaattg gaataaatgt taaaataatg gattttgaac aagaaatagc
      781 tgacaaagcc acatcattgt ataaagaagg atatatgtta tccagagtag atattgttag
      841 gaatgtattg actgaatttg aaaaattata tttagattat actgtaaata attccaaaga
      901 aaaaacctta aaaatatgta gagattattc tgcaattata gataaaaata tatatgtatt
      961 aaaaggagat aatagagaac tagtgaaatg tttagacata aatgaagagg gaaatttatt
     1021 agttcaaaga agtgatggaa gtataaaaga aattatttca ggtgaagttt ctataagagg
     1081 agaaaaagga tatgtataat aatattttgg atatagaagg cttaaaggtt gggcaggttg
     1141 aggatagaaa tggtcttact gggtgcacag tagtaatatg tgaagaagga gctacttgcg
     1201 gtgttgatgt aaggggctct gctccaggta ctagagaaac cgatttatta gaccctgtaa
     1261 atatggttca aaaagttcat gcagtagttt tggctggagg ttctgcatat ggtcttgaat
     1321 ccacttgtgg tgtaagtaga tacttagaag aaaaaggaat tggctttgat gtaggtgtag
     1381 gtaaagtgcc tatagttgta ggagcagttt tatttgattt aggagtaggg agctttaaat
     1441 gtagaccaga tttaaagatg ggttatagag cttgtgaagt tgcaaatact gatattttaa
     1501 atcaaggaaa ttatggagca ggttgtggag ccactgttgg aaagataaga ggtcaggaat
     1561 atgctatgaa aggcggaata ggtagctatt ctataaaatt agataatggt ctaattgtat
     1621 ctgcattaat tgctgtaaat gctcttggag atgtttatga aaatggtaag gttatagctg
     1681 gtgctttaaa tgataaaaag acagaaacaa ttgatacata taaattaatg aaacaaggtg
     1741 aaagtaaagg tggatttagt gtagataata ctacaattgg gataattgca acaaatgcta
     1801 agttaaccaa agctggatgt aaaaagatat ctcaaatggg acatgatgga tatgctaagt
     1861 ctatttttcc tatccataca cctcatgatg gagatacaat attcacaata tcaacaggag
     1921 aaatagaagc agatataaca ttagtaggtt cattggctgt agaggttgtg gaaaaaagta
     1981 taataaatgc agtaaaaaaa gcggatagtg tagaaaatat aatggcatat aaagatatag
     2041 taaaaaaacg agaaatatga tgaaattagc atatttttct tgtcaaattg ttgacaatgg
     2101 caactcatct tgataaaatt attcttgtta gagcgggcat cctacaatgg tagcttgact
     2161 catagttata agaaaaaaac gaaaaaaatt aaaaaattaa gtagtccggc atcttaatga
     2221 gctggaacgg aggtgtattt tttatgatga acacaagtgc atcagtaaga aaagtaaatg
     2281 taagaaagat gactataata ggagtactat cagctatttc tattatgcta tctatgactc
     2341 cattgggatt tattccagtt ggacctacaa aagcaactat aatgcacata ccagttatta
     2401 ttggagctat tatggaagga ccaatagttg gggcagcaat aggatttata tttgggatat
     2461 caagtctctt aaatgctata acaaacccaa cagtaacatc ttttgttttt ataaatccat
     2521 tagtttcaat attaccaaga gttatgatag gtgtattagc ttattatgta tatcagttaa
     2581 taattaaggc tacaaataag gtatacatat caggtttaat tacaggggca ataggttctc
     2641 tgttaaatac agctggagtt ttaggaatga tatatgtctt atatgctgac aagtatcttc
     2701 atgcaatggg taaaagtggt tcagcaggaa aagtaataat ggctcttgcc gcagcaaatg
     2761 gggtgccaga agcaatagta ggggctttag tagtagctgc tgttgccact gtacttaaga
     2821 aaagcaaaaa atagaaagaa ggttaatgac atatgcttct agtatttgat gttggaaata
     2881 ctaatatggt tttaggtata tataaaggtg acaaattagt taattactgg agaattaaaa
     2941 cagatagaga aaaaacgtct gatgaatatg gaatcctgat aagtaaccta tttgattatg
     3001 ataatgtgaa tataagtgat attgatgatg ttataatatc atctgtagtt ccgaatgtta
     3061 tgcattctct tgaaaacttt tgtataaagt actgtaaaaa acagccatta atagtaggtc
     3121 caggcataaa aacaggtcta aatataaaat atgataatcc aaaacaagtt ggggcagata
     3181 gaatagttaa tgctgtagca gggatagaaa agtatggagc accaagtata cttgttgatt
     3241 ttggaacagc aactacattt tgtgctatct ctgaaaaagg tgaatatttg ggtggaacaa
     3301 tagcaccagg aataaaaata tctagtgagg cgttatttca aagtgcgtct aaattaccta
     3361 gagtagaatt agctaagcca ggtatgacta tttgtaagag tactgtatca gccatgcaat
     3421 ctggaataat ttatggatat gttggtttag ttgacaaaat aataagtatg atgaagaaag
     3481 aattgaattg tgatgatgtt aaggttatag ctacaggtgg attagctaaa ctgattgctt
     3541 cagagacgaa aagtatagat tatgtagatg gttttttaac actagaagga ttgagaataa
     3601 tatatgaaaa aaaccaagaa taattaatat acagggaatt gggttatata tccaattccc
     3661 tttttatgat agatttctag ctatatgtgg tatattataa tgaggaaata agaatttaaa
     3721 gttaaatcac aaaataaagg caaaaatagg agaaaaacaa tgaaaatagg aaatttagaa
     3781 ttgaaaaata aagtttttct atcacctatg gcaggtgtaa cagaccttcc atttagatta
     3841 atttgtaaag aacagggatg tgggctttta tatacagaaa tgataaatgg taaggcactt
     3901 tgctatgatg atgaaaatac taaaaaaatg cttaagatag aagaggaaga acatcctgta
     3961 gcagttcaga tattcggttc agaaccagag tttatgggaa gagcagcaga gataatgaat
     4021 gattattcaa atgagattct cgatataaat atggggtgtc cagctccaaa agtagttaaa
     4081 aatggagatg gttcagcact tatgaaaaat cctaagcttg cagaagaggt attgagggca
     4141 gtagtgaaga actcaaaaaa gccagtgact ttaaagattc gaaaaggctg ggatgataat
     4201 agtgtaaatg ctgttgaaat agcaaagata gcagaggatt gtggaataag tgctttagca
     4261 atacatggaa gaactagaga acagttttat acaggcaaag cagactggga tataattgca
     4321 gagattaaga aaaatttgag tatacctgtt ataggaaatg gtgatgtatt tacaatagaa
     4381 gattcaataa atatgttaga caaaactggt tgtgatgcta taatgatagg taggggtgct
     4441 caaggtaacc cttggatatt taaaagaata aaccattata tgaatacagg cgagattttg
     4501 ccagagccaa ctcttaacga aaaaataagt actgccatta agcatttaaa acttgcagta
     4561 gaagaacatg gagagtatgt agctgtaagg gaaatgagaa aacacatcgc ttggtattta
     4621 aaaggactta gaaactctgc aaaattaagg gatgaaatta acaaaataga agactatcaa
     4681 gaagttgtgt cgaaattgga gtactatatg gaagactcct tgacataaaa ataatagtga
     4741 cttataatat agcgcataaa ttaaaaaaag attataaaat ttttaaaata taattaggaa
     4801 aattcctaag aaatttgtga aggagttgta tttataatgg aagaaaataa agagttttta
     4861 ttgacacaag agggatatga taaattagaa gaagaattag aaaacttaaa ggttgtaaag
     4921 agaaaagaag ttgcagagag aataaaagtt gctatatctt ttggtgattt atctgaaaat
     4981 gctgagtatg atgaagctaa aaaggaacaa gcacaagttg aagaaagaat tttaaaatta
     5041 gaaaatatgg ttagaaaagc tgttatcata gatgaaagca aaatagacct taatgttgtt
     5101 acaataggat caatagttaa agttaaggat ttagaatttg atgaagaggt tgaatatact
     5161 atagttggtt cgacagaagc agacccatat gatggtaaaa tatcaaacga atctcctgta
     5221 gggaaagcac ttttaggtag agctgcgaaa gaagttgtgg aagtgcaagt tcctgatgga
     5281 gttgctaagt ttgaaatatt agaaataaga agataatttg gaggaaaaaa atgaaaaata
     5341 atcaacaaag caatgaagaa gcgcaaattc aagaagacct tagtgaagta cttcaagtaa
     5401 gaagagataa actaaaaaaa ttacaagagt caggtagaga tccttttaaa gaaagtagat
     5461 atgatagaac tcattattct atggatataa aagataactt tgattctctt gagggtaaaa
     5521 ctacaaaaat agcaggacgt ataatgagca agagaataca agggaaagct ggctttatag
     5581 atattcaaga ccaagaggga agaatccaat cttatgtaag actagatgct ataggagagg
     5641 aagaatatag tgtattttca acatatgata ttggagatat agttggaatt gaaggagaaa
     5701 tctttaaaac taaaaaaggt gaaatatctg taaaggctaa atctgtagta ttactatgta
     5761 aatctttaca agttttacca gaaaaatatc atggattaaa agaccaagaa ctaagatata
     5821 gacaaagata tgttgactta atagtaaatc cagaagtaaa aaatgctttt ttaattagaa
     5881 caaaagcatt aaaagcatta agagcatact tagatgatag aggattttta gaagttgaga
     5941 ctccaatatt aaatactata gcaggtgggg caaatgctag accatttata actaatcata
     6001 atactttaca tataccaatg tatttaagaa tagcgaatga attgtattta aaaagactta
     6061 ttgttggtgg atttgataaa gtttatgaaa tgggaagaat gtttagaaat gaaggtatgg
     6121 atttaaaaca taatcctgaa tatactgcaa tagaattata tcaagcatat gcagactata
     6181 ctgatatgat ggaaataaca gaaaatgtta ttgcacatat ggctgaagtt gctacaggaa
     6241 gtatgatagt taactatcaa ggaacagaaa taaactttac accaccttgg aaaagaatga
     6301 gtatggaaga ttgtgttaaa gaatattctg gtgtagattt ttctactata aatacagatg
     6361 aagaggcact agaagttgct agagaaaaag gtatagagat aaaacctgga atgagaagag
     6421 gagaagtaat aaatgctttc tttgaagaat ttggagaaga taaattaatc caaccaacat
     6481 ttataactca tcatccagtt gaagtatctc cattatctaa gagaaatgtt gaagacccaa
     6541 gaagaactga tagatttgag gcatttgcta ataagtggga gttagctaat gcattttctg
     6601 aacttaatga ccctattgac caaaaaggaa gatttataga ccaattaaga aaaagagaat
     6661 taggtgatga tgaagctttt gaaatggatg aagatttctt aaaagcatta gaggtaggtc
     6721 tacctcctac aggaggacta ggaataggta tagatagagt aattatgtta ttaactaact
     6781 ctccatctat aagagatgta ttattattcc cgactatgaa acctattgat aacaatccaa
     6841 ataaagaaga agaaaactaa gaaatattag cattctacta agttttaaat aaagtttagc
     6901 tattaaatta tcaataaata gctaaatttt atttaaaatg tcagtgtaga atgcttttta
     6961 tatgcttcaa agattctttt agatatttaa tttataaatt accaacaaac gttagaaatt
     7021 gatattagtt tttgttgatt gagactttat atttagaaaa tatgattgaa agtaatttat
     7081 tatgaaaata tttagaataa tttatgagaa ggtaaaaaga ataatttatg agaatactat
     7141 atataaaaaa ttattggggg aatgtctgat gtttcaagta attaattttt tatgtttatt
     7201 ttgtgttgga tttttggttc ttgatgttat gatatatttt tgtaaggatg caatggatat
     7261 ttttgagggt acaactaatg ttaataaagt taagagtata aatgtttggg cagatagagt
     7321 gagagctaat tcatattctt atgaaaataa aattgcaaaa taggtaaaat tatatatgca
     7381 tgtattgttt gtaatatcaa gggttatatc tattagtttt aattatattt attaaaaaaa
     7441 acaaaagtta taaccttttt taaaataaaa aacaaaaaaa gtattgacga aa
//
LOCUS       DBGJFF010000163         7214 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.167, whole genome shotgun sequence.
ACCESSION   DBGJFF010000163 DBGJFF010000000
VERSION     DBGJFF010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7214
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.167"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(192..1031)
                     /locus_tag="ACOIAF_003458"
     CDS             complement(192..1031)
                     /locus_tag="ACOIAF_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HGZ2670438.1"
                     /translation="MSDIKNEVSKKTLSKKDVIKSWLRWFFFAQSNYNYERLQSTAFS
                     HSMLPVLKKLYPDKEELKQEVETHLAFFNTEPICGCVIHGITIAMEEEKANGADEISG
                     DGMNAIKTGLMGPLAGIGDTLTQGVITPIVLAVCIGLTEGGASIAGPILFVIAQYIIM
                     TSISFGMWTNGYKYGKKAVESILQGGIVNKVIEGASILGTLVMGGLVGRFINLSTPIS
                     YISGDFKFSLQTDLLDKIFPGLIPLVLTLLVLFALKKGLSPIKIMFILIVVGAITGIL
                     GLF"
     gene            complement(1055..1813)
                     /locus_tag="ACOIAF_003459"
     CDS             complement(1055..1813)
                     /locus_tag="ACOIAF_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HGZ2670439.1"
                     /translation="MHISLIQAVLIAIFYYLSWSPWLTYVGFFTWNRPLLAGFVTGII
                     LGDPVQGAIIGAGINMIYLGFISAGGAQMGDPAFAGYVGTALAIASKLDVSTAMAIAV
                     PLGTVATVLWIGKMTVNSFFAHWADREVQKGNVDRVAFINIVPPQILLFAMSFIPALL
                     VVYFGPNAIDGMLEVMNDNVLHVFNVIGAMLPALGIAMNLKLIGNKFTMPFFILGILM
                     AVYFKVDIIVISVIGVILALTITSIKYGKDSATS"
     gene            complement(1860..2333)
                     /locus_tag="ACOIAF_003460"
     CDS             complement(1860..2333)
                     /locus_tag="ACOIAF_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HGZ2670440.1"
                     /translation="MSNIALTRIDDRLIHGQVITAWCKITSAKRIVIVDDLVVKDPFI
                     VQVLQMAAPSTVKVEVHDVESGAEVLKSHNGDENLIVLVKYPKTVLGLVNSGVDLKEL
                     NVGGMGAGLGRKSFYKNISVSDEEKEIFRELISKDVKCFIQIVPDAKKIDVGTLL"
     gene            complement(2362..2778)
                     /locus_tag="ACOIAF_003461"
     CDS             complement(2362..2778)
                     /locus_tag="ACOIAF_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGZ2670441.1"
                     /translation="MIHMAGIIIATHGNMAKAMLESAELIVGKQENVETLGLNHGDSI
                     DEFSRKLEDGIEKYKDEGVLVLLDFYGGTPFNTSAIIINKFAKVCELECLTGVNLPML
                     LELFLNRDTMKLKDLKNLCEEVGISGIKDVKTVLKL"
     gene            complement(2825..3877)
                     /locus_tag="ACOIAF_003462"
     CDS             complement(2825..3877)
                     /locus_tag="ACOIAF_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HGZ2670442.1"
                     /translation="MKSVRFYGIRDTRVEDVDVPKILEKDDVIIKVKVAGICGSDISK
                     YSKTGPHMVGEILGHEFSGEVAQVGKEVRSFKIGDRVAVCPAMPCFECDECKKGLYSR
                     CNNVAIIGNKELGGCFAEYTKVKERNLIKIPDEISYETAAALEPVCIAGHGLFRSEAK
                     VGDTVVVLGTGPIGLFSIQWAKIFGSTKIIAVDVFDEKLDLAKELGADICINAKEKNI
                     VEEIKRLTDGDGADIVIESAGTPLTCGQVLLLAKKGGTVLYAGVPYGDVALTREQFEK
                     IVRSELTVKGTWFGNSFPFPGKEWSAGLYHMQKGDMNVEKLVTHRINLEEVPAYFEKV
                     YKRDIFFGKIMINIDN"
     gene            4273..4953
                     /locus_tag="ACOIAF_003463"
     CDS             4273..4953
                     /locus_tag="ACOIAF_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HGZ2670443.1"
                     /translation="MKKVYQGKTKDVFELDNGNYLLKFKDDVTGKDGVFDPGENSVGL
                     SIDGIGRANLETSVKFFEILNNAGIKTHYVSANLEEATMEVLPAKVFGNGLEVICRYR
                     AVGSFLRRYGSCVEEGAKLDCYVEATLKDDDRCDPLITSEGLEALNIMTQAQFDSMKS
                     MTQKISNIVRDTIAEKGLELYDIKFEFGFYNDEVILIDEIASGNMRVYKDGVIVDPMD
                     LTALLLDK"
     gene            complement(5086..5406)
                     /gene="sugE"
                     /locus_tag="ACOIAF_003464"
     CDS             complement(5086..5406)
                     /gene="sugE"
                     /locus_tag="ACOIAF_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HGZ2670444.1"
                     /translation="MKWLFLFVAGLFEVFWAFQLKNSHGFSKLTPSILTVVGMIVSFY
                     FLSLALKHLPLGTSYAIWTGIGTVGTAIVGMAILNEPISISRIACIGFIVVGIVGLKL
                     LSTN"
     regulatory      complement(5476..5582)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00442"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="guanidine-I (ykkC/yxkD leader) riboswitch;
                     Guanidine-I riboswitches control genes whose products are
                     involved in modification or pumping out guanidine as a
                     toxic compound from bacteria; This is class of guanidine
                     riboswitches is based on the ykkC/yxkD leader; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="guanidine"
                     /db_xref="RFAM:RF00442"
     gene            5788..6615
                     /locus_tag="ACOIAF_003465"
     CDS             5788..6615
                     /locus_tag="ACOIAF_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HGZ2670445.1"
                     /translation="MNFYKIAVCQMITTENKIENINHAVDMVTEAAINGAKIVVLPEM
                     FNCPYENKYFPKFAEEYPGETTTILSKLAEKHGIYLVSGSIPELEDGKIYNTCYVFDK
                     NGALIGKHRKMHLFDIEVTGKVSFKESDTLTAGNDVTVIDTEYGKVGIAICYDIRFPE
                     LSRLMALKGAEIVILPAAFNMTTGPAHWELSIRMRALDNQIFYVGAAPARNMNASYIA
                     FGNSRISDPWGRIIAQADEKECIIYADIDRDLIPDIRQQLPLLKHRRTDLYELNTLK"
BASE COUNT         2440 a         1310 c          843 g         2621 t
ORIGIN      
        1 cctcattctg attaaaaatc tttctgagat actttttaaa atattaaata ctacatatag
       61 cttacataag tttaaaattt aatctattat aaaattaccc taattgccaa actttttaat
      121 ataaattttc caacatccta caaatataat caatagatat agaatcctaa cttacaccaa
      181 tatatattta tttaaaatag tcctaaaata cctgtaatag ctcccactac tatcagtata
      241 aacattattt ttattggaga aagacctttt tttaaagcaa agagtactaa taaagtaagt
      301 accaaaggaa ttaatcctgg aaatatctta tcaagtaaat ccgtttgaag actaaattta
      361 aaatcaccac taatataact tattggtgta gataaattga taaacctacc tacaagcccc
      421 cccatgacta aggtacctag tatagaagcc ccttctataa ccttatttac tattccacct
      481 tgaagtatac tttcaacagc ttttttccca tacttatatc catttgtcca cattccaaaa
      541 gaaattgaag tcattattat atattgagct attacaaata atataggtcc tgcaattgat
      601 gctcctccct cagttaaacc aatacaaaca gccaacacta taggagttat aactccttga
      661 gttaaagtat ctccaattcc tgctagtgga cccatcaagc ctgttttgat agcattcata
      721 ccatctccac ttatttcatc tgctccatta gctttttctt cttccatagc tattgttatt
      781 ccatgtataa cacatccaca tataggttct gtgttaaaaa atgctagatg agtctccact
      841 tcttgtttta actcttcctt atctggatat aattttttaa gtactggcaa cattgaatgt
      901 gaaaaagctg ttgattgcaa tctttcataa ttgtaattag attgtgcaaa gaaaaaccat
      961 cttaaccatg attttattac atctttcttg cttaacgttt tcttagatac ttcatttttt
     1021 atatctgaca taatattttc cccctcacat gtatttaaga tgtagcacta tcttttccgt
     1081 acttaattga agtaattgta agagcaagta ttactccaat aacagatatt actattatat
     1141 ctactttaaa ataaacagcc attagtattc ctaatatgaa aaagggcata gtaaacttgt
     1201 ttccaatcaa ttttaaattc atagcaatcc caagtgctgg caacatagcc ccaattacat
     1261 taaatacatg taacacatta tcattcatta cttccaacat accatctatc gcatttggac
     1321 caaaataaac aactaataat gctggtataa aactcattgc aaataacagt atctgtggtg
     1381 gtactatatt tataaatgct actctatcaa cattcccttt ttgaacctct ctatcagccc
     1441 aatgtgcaaa aaaagaattt actgtcatct ttccaatcca aagtacagtt gcaacagttc
     1501 ccaatggtac agctattgcc attgcagtac ttacatctag cttagatgct atagctaaag
     1561 cagtacctac atatcctgca aaagctgggt cccccatctg agctccccct gctgatataa
     1621 aacctagata tatcatattt atacctgctc ctataattgc tccttgaaca gggtctccta
     1681 atataattcc cgttacaaat ccagccaata gaggtctatt ccatgtaaaa aatccaacat
     1741 aagtcaacca cggactccaa gaaagataat aaaaaattgc tattaatact gcttgaatta
     1801 gtgaaatgtg catattcttc ctccctattt tcttttaaag atttacaaga tagtcttctt
     1861 tataataatg ttcctacatc aattttcttt gcatctggta ctatttgtat aaagcattta
     1921 acatcttttg atattagctc tctaaatatt tctttttctt catctgacac agatatattt
     1981 ttataaaaac tttttctacc aagccctgct cccatacctc caacattcaa ttcctttaaa
     2041 tctaccccgc tatttactaa acctaagaca gttttagggt atttgaccaa tactattaaa
     2101 ttttcatctc cattatgtga ttttaagact tcagcacctg attctacatc atgtacttca
     2161 accttaacag ttgatggagc tgccatttgt aaaacttgaa ctataaatgg gtcctttact
     2221 actaaatcat ccacgattac tatccttttt gccgatgtaa tcttacacca agcagtaata
     2281 acttgcccat gtattaatct atcatcaatt cttgttaatg ctatattact cataattatt
     2341 cctccagatt tttaatttat tttacaactt taaaacagtt ttaacatctt tgattcctga
     2401 tatcccaact tcctcacata aattcttcaa gtcttttaac ttcatagtat ctctgtttaa
     2461 aaataactct aaaagcatag gtaaatttac acctgtcaaa cattctaact cacatacctt
     2521 agcaaattta tttattataa tagcagatgt attaaatgga gttccgccat aaaaatcaag
     2581 caatactaaa accccttcat ccttatattt ctcaattcca tcttctaact ttctactaaa
     2641 ctcatctatg ctgtcaccat ggtttaatcc taaagtttct acattttcct gtttgcctac
     2701 tattaattca gcactttcta acattgcttt agccatattt ccatgtgtgg ctataattat
     2761 tcctgccata tgaatcatac ctttcttaac cttttttata tggattatta tttagtactc
     2821 taatctaatt atctatatta atcattatct taccaaagaa aatatccctt ttgtatactt
     2881 tttcaaaata agctggtact tcttctaaat ttattctatg tgttactaat ttttcaacat
     2941 tcatgtcacc cttttgcata tgatacaatc cagcactcca ttcttttcct gggaatggaa
     3001 aggagtttcc aaaccaagtt ccctttacag ttaattcact tcttactatc ttttcaaatt
     3061 gctctctagt caaagctaca tccccatatg gtacacctgc atacaatact gtacctccct
     3121 ttttagctag caacagtact tgaccacatg tcagtggagt accagccgat tcaataacaa
     3181 tatctgcacc atctccatca gttaaccttt ttatttcttc tacaatattc ttttcttttg
     3241 catttataca aatatctgcc cctaactctt ttgccaagtc taacttctca tcaaatacat
     3301 ccacagctat tatttttgta gaaccaaata tttttgccca ttgtattgaa aataatccta
     3361 ttggacctgt accaagtact acaacagtat ctccaacttt agcttctgac ctaaaaagac
     3421 catgtcctgc tatacatact ggttctagcg cagcggcagt ctcataactt atctcatctg
     3481 gtatttttat taaatttctc tctttcacct ttgtatactc tgcaaaacaa cctccaagtt
     3541 ctttatttcc tattattgct acattattac atcttgaata caagcctttt ttacattcat
     3601 cacattcaaa acatggcata gctggacaaa cagcaactct atctcctatt ttaaaactcc
     3661 ttacttcttt accaacctgt gccacttctc cagaaaattc atgccctaaa atttctccaa
     3721 ccatatgtgg acctgtcttt gaatattttg atatatcaga accacatatt ccagctactt
     3781 ttactttaat tattacatca tctttttcta atatttttgg tacatccaca tcctccactc
     3841 ttgtgtctct tataccatag aatctaactg acttcactat taaccacccc tactttttat
     3901 ttgagaatat atccctattg tttatatgtt aaatatctaa agcaactttc atgccaatat
     3961 atgtaagcat tgtattcatg cattttataa aattatcatt aaatgtgtgt caaagtgtgt
     4021 caaaagtttc ttatattaaa acttatgtgt gtcaatacaa acgacactaa cattttatat
     4081 tagttctctt attataagtt ttataaaatg tacttttttt gttattttta taaaattgtt
     4141 cggaaatttc ccttctattt gtgatataat attaaatata aggtatcaat ataaattttc
     4201 atgcgaatat cagaactttt ttgacactaa tgccttattc aatataataa tttaactttt
     4261 tggaggttgt ttatgaaaaa agtgtatcaa ggaaagacta aagatgtttt tgaattagat
     4321 aatggaaatt acttacttaa atttaaagac gatgtaactg gtaaagatgg agtatttgac
     4381 ccaggcgaaa atagtgtagg tctaagtata gatggaattg gtagagctaa tttagagaca
     4441 tctgttaagt tctttgaaat tctaaacaat gctggaatta aaactcatta tgtaagtgct
     4501 aatttagaag aagcaactat ggaagttcta ccagcaaaag tttttggtaa tggattagaa
     4561 gttatttgtc gttatagagc tgtaggtagt ttcttacgtc gttatggttc atgtgttgaa
     4621 gaaggtgcta aactagattg ttacgtagaa gcaactttaa aagatgatga ccgttgtgac
     4681 cctcttataa catctgaagg tttagaagct ttaaacatta tgactcaagc tcaatttgat
     4741 tcaatgaaaa gcatgactca aaaaatttct aacatagttc gtgatactat agctgaaaaa
     4801 ggtcttgagc tttatgatat taaatttgaa tttggattct acaatgatga agtaatctta
     4861 atagatgaaa tagcatctgg aaatatgcgt gtttataaag atggtgtaat agttgaccct
     4921 atggatttaa ctgctctttt attagataaa tagtatatac atataattta caaatataat
     4981 aaacttataa ataagataag ctatgaagta aagacttttt aaatgttgat tatttttaaa
     5041 cattttctcg cctttaattt catagcttat ttaaattaat atactctaat tagtagataa
     5101 tagctttaac cctacaattc ccaccacaat aaaacctata catgcaattc ttgatatact
     5161 aataggctca tttaaaatag ccatgcctac aatagctgta ccgacggtac caattccagt
     5221 ccatattgca tatgaagttc caagtggaag atgttttagt gctaatgaca aaaaataaaa
     5281 acttacaatc attccaacaa cagtaagtat acttggagtc aatttagaaa atccatgtga
     5341 attttttagt tgaaaagccc aaaacacttc aaacaaacct gcaacaaaca aaaataacca
     5401 tttcataaat tcatccctct tttcaaatat gtctcttatt tttccaataa aataacctga
     5461 gaaatattat aaaatatctc ccaggttttt gtccttccgt gttcacaaca aaaactatgt
     5521 tgtgggtttt atctcggacc agaccaatca ttaataattg cggaacccta taaaactcta
     5581 ttaattattc tattttatct ataataatac tatcaacata tataaaaaaa gtcaatatat
     5641 gggttccatc tataataaaa ataactttta tatacctttt aatattctta ttcacttctc
     5701 tccataccca tttaggtcaa tctaagaata tctaattttt atttaatatg ttataataac
     5761 ataaaaatat tgaaaggagt aatttctatg aatttctata aaattgcagt atgtcagatg
     5821 attactacag aaaataaaat tgaaaatatc aatcatgctg tagatatggt aacagaagct
     5881 gccatcaatg gagctaaaat tgtagttttg ccagaaatgt ttaactgtcc atatgaaaat
     5941 aaatattttc ctaaatttgc tgaagaatat ccaggtgaaa caacaacgat attaagtaaa
     6001 ttagctgaga aacatggaat ttatctagtt tctggtagta ttcctgagtt agaagatgga
     6061 aaaatctata atacttgcta tgtatttgat aaaaatggag ctctaattgg aaaacatcgt
     6121 aaaatgcact tatttgacat tgaagttaca ggaaaagtta gttttaaaga atcagatact
     6181 cttacagctg gaaatgatgt cactgtaata gataccgaat atggaaaagt tggtatagct
     6241 atttgctatg atattcgttt ccctgagtta tcacgtttaa tggctcttaa aggtgctgaa
     6301 atcgttatct tacctgctgc atttaatatg actactggtc ccgcccattg ggagttatct
     6361 ataagaatgc gtgctttaga taatcagata ttctatgttg gtgctgcacc tgctagaaat
     6421 atgaatgctt cttatatagc ttttggaaat tcaagaattt cagacccttg gggaagaata
     6481 attgctcaag ctgatgaaaa agaatgtatt atttatgctg acattgatag agatttaatt
     6541 cctgacataa gacaacagtt accacttctt aagcacagaa gaactgattt atatgaactt
     6601 aatactttga aataaaacag aacataaccc cataaatcgt aaattaagtg ttaaaaaaat
     6661 ttatggggtt ctcattaatt tgatttaaaa ttaatttcat aaaaataatg ctaatattaa
     6721 tttaattaaa taatactaat aattactaat gattatgcta attatcatca tttactaatt
     6781 taatcactat tagttatttc ttttattaaa tatctttact tttttactta ttatgtcttc
     6841 tagctttaca tttggattat ggtatttctt tctattttta gtcacatctt tctattgaat
     6901 tacttgctta aaacaccaat gcacacatgc cagataatgt tcacaattat gtagaaatct
     6961 tgctctacct tcttcaatgt tgatattatc tctcctaaaa tttcttttaa ttaaaaaata
     7021 acattagttg atatatcaag tatattttaa gcaactgaaa aaaacaataa taattgatat
     7081 atcaactaat taatgtagat taattcattt ttataattta tatacttttt aattaatcta
     7141 ccttatttaa ttaaaatcct tcctttctat ttatccattt tcaacttaaa ttataagatt
     7201 tcatcagcta tgtt
//
LOCUS       DBGJFF010000164         7190 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.168, whole genome shotgun sequence.
ACCESSION   DBGJFF010000164 DBGJFF010000000
VERSION     DBGJFF010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7190
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.168"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(265..1515)
                     /gene="cme"
                     /locus_tag="ACOIAF_003466"
     CDS             complement(265..1515)
                     /gene="cme"
                     /locus_tag="ACOIAF_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Cme"
                     /protein_id="HGZ2670446.1"
                     /translation="MENKNTNSTWKKKVILFLASQYISLFGSSLVQMAIIWYVTLQTS
                     SGVWVTVLTVCSFIPQMIISFFAGVWADYYNRKVLIIVSDSTIAISTLVLILFMMSSN
                     GTTSELIAISVISVIRSLGSGVQTPAVNAMIPQLVPEEHLMRFNGINSSLQSIVQFIV
                     PIVAAAILGFGNKINQVLLIDVFTAIIAIVLLIFIKIPKHDIPKQTEKVSFFADMVSG
                     IKYCFSDKFIRKVLFTYGAFMLLCVPSGFLIGLLIERTFGKGYIYLSIIEMIGFLGMV
                     LGGLIIGVFGGFKNRNKTFFLAILSYAIFSIILGLVTQAWQFFIFMFFTNFSIPIIQA
                     SAMTMLQEHVAPQMLGRVLSLPIIIYTGFIPLGMMIFGPMADVISINNLIIIAGIILL
                     FFALAIPYSKQFYKQGISKSNESE"
     gene            complement(1601..2347)
                     /locus_tag="ACOIAF_003467"
     CDS             complement(1601..2347)
                     /locus_tag="ACOIAF_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HGZ2670447.1"
                     /translation="MDTYTTSEVADLIGVHPNTVRLYEELNLIPKPKRNTNGYRIFND
                     FHIEQFRFARTALKIEVVQRGLRKDALEIVRTSANRDFDKAISLVNQYLQQLEIEKSN
                     AEEALTIVAQILNGNTPETNKLFLTRKETAEHLNVTIDTLRNWEMNGLLKVKRKQNGY
                     RVYTDEDIKRLKIISALRCANYSLSAILRMLNALSSNPNTNIKTVLDTPKDDDEIISV
                     CDKLITSLKEAEHNAKTMKSHLQIMKKQFS"
     gene            complement(2519..3541)
                     /locus_tag="ACOIAF_003468"
     CDS             complement(2519..3541)
                     /locus_tag="ACOIAF_003468"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HGZ2670448.1"
                     /translation="MNKNNCLDLIFEKVDPSSVGMSSEKLSQLEPLIISQYSNINGII
                     IARKGKIVFEKYYNGYNADDTFHVASVTKSIISALIGIAIDKGYIKDVDQKVVDFFPE
                     YTCSPSEICKKAVTIRHLLTMTAPYPFKNMTESLERLCKQKDWIKYTLNQLGQKGEIG
                     TFKYSSAGTHLLSAIITKTTGKSAREFANEYLFKPIGMKVIPDYDMKDFGFEDFFGKN
                     LKGWAKDRQGYSIGGWGLTLSARDMLRFGFLYLNDGIWNNKRIISKSWIEESVKMNSN
                     EYGYLWWLCKKDGVLWYSAMGTGGNAICCIPQYDLVIAIPSKIVFKPRDRWEFIEKYI
                     LTSIID"
     gene            complement(3541..5961)
                     /locus_tag="ACOIAF_003469"
     CDS             complement(3541..5961)
                     /locus_tag="ACOIAF_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HGZ2670449.1"
                     /translation="MKEIKINGAKIHNLKNIDVSIPKDKLVVATGVSGSGKSSLMFDI
                     VFEEGRKQYLQSLGILAGIDSEDKFDNIQGLAPTIAVQQNIIRQSNPRSTVGTRTNIL
                     NMLTLLYSVEGQIMCTMCDTPVSDNLICNNCGYEEERLRPSYFSYNSSDGMCMKCSGH
                     GAYFEINIEKLVLDKHDTLKQVLDRAKITPGYLRVFSKKFNDYLDIQFLQLPEEIRNE
                     VLYGHYENGKKSSSLSKVFHNRYKRGEDLNGVYTMTACSDCNGFKIGEEARRVLLNGK
                     HIGELGKMTILEIDDFLKLLINQGNLNTFGTNLVNDILSKTQHLIESRLGHLSLYREM
                     STLSGGEMQRLFLNSHLDSEMDSLIYVLDEPTAGLHESEKIDILKSIKKLKDLGNTVI
                     VVEHDKNTIKMAEHIIDIGPKAGVEGGQLVYQGDLEGLLQSDKSLTGQYLSGKYPMPN
                     RTSNKNITHTDKIPCITIQNANTNNLKNVTVSLPLGAMVGISGKSGSGKSSLISDTLL
                     PLLRSQFNNHTINNQINSSESEIGEEDDYAVVETIADRLIGTKHISGYSEISQSPIGK
                     NMNSTPVSYIGIWDKIRTLFAGQPESLERGFTAGHFSFNSKGACPKCSGSGYEKVWLG
                     NNLSIDHICSECHGKRFNDESLSIKYKNKNIHDILNMSVSESVHFFKDIPNIVSHLNV
                     LEQIGMGYIKLGQPTPTLSGGESQRIKLAKEIGKKRNGNILYVLDEPTTGLSLYDTSK
                     LIQLLDELVENGNSVIVVEHDIDVLNVCDWIVELGPEGGDKGGYIIAEGSPKTLKENP
                     KSITGRYL"
     gene            complement(6047..6967)
                     /locus_tag="ACOIAF_003470"
     CDS             complement(6047..6967)
                     /locus_tag="ACOIAF_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HGZ2670450.1"
                     /translation="MTNAKSKKEYLNRICKVQDYIEEHLHEPLSLDELSNIAGFSKFH
                     FHRIFKAIEKETLAQYVNRLKLENATAFLIHRTDMTVTDIAHYFGFTDSAVFSRAFKN
                     YYKISPIKYRNNYSKNCKEPYKISQYNRSISKPKCKNTREVKGEIEILELDNMNTIYT
                     RYTGSYGNLTSTFPKLLERLFTYASEQNLLGLENTQILAIYHDNPQFTQEHQLRTSIC
                     MTIPDNIVVKENDNLGNMIIPSGKYLVGHFDISKSEYSDAWDFMYNEWLSNGNYKLRA
                     SFPFEVYLNNPHNHPQNRHLVDIYLPIEPF"
BASE COUNT         2388 a         1154 c         1030 g         2618 t
ORIGIN      
        1 atggaggtat tattatatga tttgataaat ttcatcatta taacctacaa gtaaaggtat
       61 tattatataa agttaataaa gatagtatca aaatattatt tgctataaag agagggactc
      121 aaaataaaga taaacctatt tttcaatgtt gaaatgataa aatatatttg atttcatata
      181 aaaaattttc ttcataaatt aaaaagtcca ttttaagccc cctcattttt aaccagataa
      241 atcgagttaa actatttaaa tatattactc ggattcattt gacttagata ttccttgctt
      301 gtaaaattgt tttgaatagg ggatagccaa agcaaaaaat aaaagtataa tccctgctat
      361 gattattaaa ttattaatag aaattacatc tgccattggt ccgaaaatca tcataccaag
      421 aggaataaaa ccagtatata taataatagg taagctaaga actcgaccta gcatttgtgg
      481 tgctacatgc tcttgcaaca tagtcatagc tgatgcttga atgattggaa ttgaaaaatt
      541 cgtaaaaaac ataaatataa aaaattgcca agcttgtgtt acaagaccta gaataattga
      601 aaaaatagca taggataata ttgctagaaa aaaggtctta tttctatttt taaaaccacc
      661 aaaaactcct ataatcaatc cacctaaaac catacctaaa aatccaatca tttcaataat
      721 agacaggtat atataaccct tgccaaatgt tcgttcaata agaagtccta taagaaatcc
      781 tgatggaaca caaagaagca tgaatgctcc ataagtaaaa agcacttttc taatgaactt
      841 atctgaaaaa cagtacttta tccctgatac catatctgca aaaaatgaga ctttttcagt
      901 ttgttttggt atatcatgtt taggaatttt gatgaatatc aataatacaa tagcaataat
      961 tgcagtaaat acatcaatta aaagaacttg atttatttta ttaccaaatc caagtattgc
     1021 agcagctaca ataggaacta taaattgtac tatagactgt agactactat ttataccatt
     1081 aaaacgcatt aaatgttctt ctggtacaag ttgaggaatc attgcgttta cagcaggtgt
     1141 ttgtacacca cttcctagtg aacgtataac agatattact gatatggcta ttaattcact
     1201 tgttgttcca tttgatgaca tcataaatag aattagaact aatgtagata tagcaattgt
     1261 tgaatctgat acaattatga gtacctttcg attgtagtag tctgcccata caccagcaaa
     1321 aaaagaaatt atcatttgag gtataaagga gcatacagtt aaaactgtta cccatacacc
     1381 tgatgatgtc tgaagtgtga cataccatat tattgccatt tgtacaagtg atgagccaaa
     1441 taaggaaata tattgacttg ctaaaaaaag aatgactttt ttcttccatg tagaattagt
     1501 atttttgttt tccatcctat tcctcctttg atttgtgtgt tgaatttttc ttgctcttaa
     1561 ggataacaca caaatcaaag tctggagtta gagtggaggg ctaggaaaat tgttttttca
     1621 taatttgaag atgagatttc atggtttttg cattatgttc agcttctttc aatgaagtta
     1681 tcagtttgtc acacactgag ataatttcgt catcatcttt tggagtgtca aggacagttt
     1741 taatattagt attaggatta gaagataaag catttaacat tcgtaaaata gctgaaagag
     1801 aatagtttgc acaacgaagt gcgcttataa ttttaagtcg ttttatatct tcatcagtat
     1861 atacacgata accattttgt tttcttttta cttttaacaa accattcatt tcccaatttc
     1921 ttaaagtatc tattgtaacg tttaagtgtt ctgcagtttc ttttctagtt aaaaagagtt
     1981 tatttgtttc tggagtattt ccatttaaaa tctgtgcaac aatagtaagt gcttcctcgg
     2041 cattagattt ttctatttct aattgttgca agtattgatt tacaagacta atagccttat
     2101 caaagtccct attcgctgat gttcttacaa tctcaagggc atcttttctt aatccccttt
     2161 gtaccacttc aattttcaag gctgtacgag caaatctaaa ttgttctata tgaaaatcat
     2221 taaagatacg ataaccattg gtattacgct taggtttagg tattaagttt agttcttcat
     2281 ataaacgtac tgtattagga tgtacaccaa ttaaatctgc tacctctgat gtcgtataag
     2341 tgtccattag tattcaactc cttttagata tatagtatta gtcataataa caccaaacaa
     2401 aagtctggtg ttataatgga aggtttggct atctaagagt aattagttta tgaaagagta
     2461 attagtttat gaaagagtaa ttaatttatg aaagagtaat taatttatga aagagtaatt
     2521 aatctattat agaagtaagt atatattttt caataaactc ccatctatca cgaggtttaa
     2581 acactatttt agatggtatg gctattacta agtcatattg tggaatacaa caaatagcat
     2641 ttcctccagt tcccattgct gaataccaca aaacaccatc ttttttacat agccaccaca
     2701 gataaccata ttcattacta ttcattttaa ctgattcttc aatccacgat ttagatataa
     2761 tacgtttatt attccaaata ccatcattta aatacagaaa accaaatcgc aacatatctc
     2821 tagcactcaa tgtaagtccc cagccaccta tagaataacc ctgtctatct tttgcccaac
     2881 ccttaagatt ttttccaaag aaatcttcaa aaccaaaatc tttcatatca taatctggta
     2941 taactttcat accaataggt ttaaataagt attcattagc aaattcacga gcagattttc
     3001 cagtagtttt tgttataata gctgaaagta ggtgcgttcc agctgaagaa tatttgaaag
     3061 taccaatttc gcctttttga ccaagttgat tcaaggtata ttttatccaa tctttttgtt
     3121 tgcaaagtct ttctaaagac tcagtcatat ttttaaaagg gtatggtgca gtcattgtaa
     3181 gtaggtgacg tatagttact gcttttttac atatttcaga aggactacaa gtatattcag
     3241 gaaaaaaatc aacaaccttt tggtcaacat cttttatata tcctttatct attgcaatac
     3301 ctattaaggc tgatataata ctttttgtaa ctgatgcaac atgaaaagta tcatcagcat
     3361 tatatccatt atagtacttt tcaaagacaa tttttccttt tcttgcaata attataccat
     3421 ttatattgct atattgtgag attattaaag gttcaagttg tgaaagcttt tctgaactca
     3481 taccaacact tgaagggtct actttttcaa aaattaagtc taagcaattg tttttattca
     3541 ttaaagatac ctccctgtta tagattttgg attttctttt agagtttttg gagaaccttc
     3601 tgcaattata taacctcctt tgtcaccacc ttctggacca agctcaacaa tccagtcaca
     3661 aacatttaat acatctatat catgttcaac aacaattaca gaatttccat tttcaaccaa
     3721 ttcatctaat aattgaatca acttagaagt atcatataga cttagaccag tagttggctc
     3781 atccaataca taaaggatat taccatttcg ttttttacca atttcttttg caagtttgat
     3841 tctttgggat tcaccaccac ttaaagtcgg tgtaggttgg cctaatttta tataccccat
     3901 acctatttgt tctaatacat ttaagtgtga aacaatattt ggaatatctt tgaagaaatg
     3961 tacagattca gaaacactca tatttaaaat atcatgtata tttttatttt tatatttaat
     4021 agataaagat tcatcattaa atctttttcc atgacattca ctgcatatat ggtctatact
     4081 tagattattt cccaaccaga ccttttcata tccactacca ctacatttag gacaagcacc
     4141 tttggagtta aatgagaaat gaccagctgt aaatcctcgt tctaacgatt ctggctgacc
     4201 tgcaaatagt gtacgtatct tatcccatat accaatataa gaaacaggag ttgagttcat
     4261 atttttgcct ataggagatt gagatatctc tgaatatcca gaaatatgtt tagtaccaat
     4321 cagtctatct gcaatagttt caacaactgc ataatcgtct tcctcaccta tttcactctc
     4381 tgaagaattt atttgattat tgatagtatg gttattaaat tggcttctaa gaagaggaag
     4441 tagggtgtct gatataagtg aactcttgcc actacctgat tttccagata ttccaaccat
     4501 agcacccaaa ggtaatgata cagttacatt tttaagatta tttgtatttg cattttgaat
     4561 agttatacaa ggtattttat cagtatgtgt aatgttttta ttgcttgttc tattaggcat
     4621 tggatatttt ccagatagat actgtccagt aagtgatttg tcggattgaa gtagaccttc
     4681 taaatctcct tgatatacta attgaccacc ttcaacacca gcttttgggc caatatcaat
     4741 aatatgttct gccattttaa ttgtgttttt atcatgctca actactatga ccgtattgcc
     4801 taaatcttta agtttcttta ttgattttaa gatgtctatt ttttctgatt catgcaatcc
     4861 agctgttggt tcatcaagca cataaattaa tgagtccatc tctgaatcga gatgtgaatt
     4921 taaaaatagt ctttgcattt ccccaccact caaagttgac atttctcggt ataaggatag
     4981 atgacctaat cgtgattcta ttaaatgttg agttttggat aaaatatcat ttacaagatt
     5041 tgtaccaaat gtgtttaaat taccttgatt tattaagagt ttaaggaaat catcaatttc
     5101 taaaatcgtc atttttccaa gttctccaat atgttttccg tttaaaagta ctctacgtgc
     5161 ttcttcacca attttaaaac cattgcaatc agaacaggca gtcatagtat aaactccatt
     5221 taaatcttcg ccccttttgt aacgattgtg aaataccttt gataaagagg aacttttttt
     5281 accattttca taatgaccat agagtacttc atttcttatt tcttcaggta gttgtaagaa
     5341 ttgtatgtct aaataatcgt tgaatttttt agaaaatact ctcaaatatc ctggtgtaat
     5401 ctttgctcta tctaagactt gttttagggt atcatgctta tcaaggacta atttttcaat
     5461 attaatctca aaatatgcac catgtccaga gcacttcata cacattccat ctgaagagtt
     5521 gtatgagaaa taacttggtc ttaatcgttc ttcctcatat ccacaattat tgcaaattag
     5581 attatcgctt acaggagtat cacacatagt acacattatt tgcccttcca ctgaatacaa
     5641 tagtgtaagc atgttgagaa tattggttct tgtgcctact gttgaacgag gattgctctg
     5701 gcgaataata ttttgttgaa cagctatagt gggagctaaa ccttgaatat tatcaaactt
     5761 atcttcacta tcaatacctg caagtatacc taaagattgc agatattgtt tacgcccttc
     5821 ctcaaataca atgtcaaaca taaggctaga cttacctgag ccactgacac ctgttgcaac
     5881 aacaagctta tcttttggaa tagaaacatc tatattttta agattgtgta tctttgctcc
     5941 attaattttt atttctttca ttggttcacc tcattttatc aattttatca aaccgtaaaa
     6001 tagggatata ttaatcctta atacaagaat agattaggtg aggttattaa aaaggttcta
     6061 taggtaaata tatatcgacc aagtgtctat tttgagggtg attgtgtggg ttgttaagat
     6121 atacttcaaa agggaaggag gcacgtagct tataattacc atttgatagc cattcattat
     6181 acataaaatc ccacgcatca ctatattcag attttgaaat atcaaaatgt cccacaagat
     6241 attttccaga aggtattatc atattgccaa gattgtcatt ctctttgaca actatgttat
     6301 ctggaatagt catgcaaata ctagtcctta attgatgctc ctgtgtaaat tgtggattat
     6361 catgataaat ggctaatatt tgtgtgtttt caagtccaag tagattctgc tcacttgcat
     6421 atgtgaataa ccgttctaaa agttttggga aagtactggt tagattacca tatgaaccag
     6481 tatacctagt gtaaatagta ttcatattat caagttctag aatttcaatt tcacctttaa
     6541 cctctctagt attcttacat ttaggttttg atatgcttct attgtactga gaaattttat
     6601 atggttcttt gcaattcttg ctataattgt ttctatattt tatagggctt attttatagt
     6661 aatttttaaa tgctctcgag aaaactgctg aatcagtaaa gccaaagtag tgtgcaatat
     6721 cagtaacagt catgtcagtt ctatgaataa gaaaagcagt tgcattttct agttttagcc
     6781 tatttacata ttgtgccaaa gtttcttttt ctattgcttt gaagattcta tgaaaatgaa
     6841 attttgaaaa acctgctata ttagaaagtt catcaagaga aagaggttca tgtaaatgtt
     6901 cttctatata gtcttgcact ttgcaaatac gatttagata ttctttttta cttttagcat
     6961 ttgtcatata tagccctcct tattgtgtaa aatgtgtatc aattaattta tagcaatata
     7021 gtaataaaat tttatgtttt tgtaatttat gataaataag atagcgttta attttttata
     7081 attgataaaa gatatttata attaataaaa gatataagat gccttatatt tgtctttgca
     7141 tataagtata gtcttcgtaa taattagtat accatagtga tttattttta
//
LOCUS       DBGJFF010000165         7061 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.169, whole genome shotgun sequence.
ACCESSION   DBGJFF010000165 DBGJFF010000000
VERSION     DBGJFF010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7061
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.169"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            724..1998
                     /locus_tag="ACOIAF_003471"
     CDS             724..1998
                     /locus_tag="ACOIAF_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HGZ2670451.1"
                     /translation="MQFIDVSKYIEGANLKVKTKNGETVKRIYFNNSATPLVLKNVMD
                     NLNCEIPWLTYINAPGIISEKNTLKYENVRSTILKLIDGDEEKDSVIYVKNATEGINF
                     LAKFFKDENPDKFVITTAMEHMANYLPFKVNFPTKVVGITEKGELDLCQLENILKNYA
                     GNVSLVTVTGASNVTGITTPIYEIARIAHKYGAKILVDIVQVIQHKPFSMMPHECDEH
                     IDFVVFSAHKCYSPLNGGALVGPKKFFEKFNPVLYGSGSTKFVNDNKILYADIPQRFE
                     AGYPEYFGTLAMGKALNTLNKIGLNRISKYERELFLYVKEEMSKIPNIIIYGDKSNNV
                     IIPNISFNISNMYYKDVAKYLVDNFGIETGAGVVGADIYVQKLLGISPKEAYVRFMRE
                     NPVGLVRISLGMYNTFDEVDSFIHAIKTLAFK"
     gene            complement(2205..2522)
                     /gene="trxA"
                     /locus_tag="ACOIAF_003472"
     CDS             complement(2205..2522)
                     /gene="trxA"
                     /locus_tag="ACOIAF_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HGZ2670452.1"
                     /translation="MVKIINNNEFINEVENKDGLVVVDFFATWCGPCKMLSPIYEALG
                     NEMVEKANFLKVDIDQSIELAQKFEVSTVPTMLIFKDGKPVDRLIGFMPKENLKNKIE
                     SYM"
     gene            2789..3541
                     /locus_tag="ACOIAF_003473"
     CDS             2789..3541
                     /locus_tag="ACOIAF_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HGZ2670453.1"
                     /translation="MDNIINELQNIGFTKYESQIYISLLKESPLTGYEISKLSGVPQS
                     KVYENITKLLNNNIIINVGTDPIKYIPINPNELLKNKKKEFDNSMDNLKNSLQILNKS
                     KSVEYVLNIKGDKNILKKAIEMIKNAKQEIIISCPYEEILELKKELLIAYKKNINIEI
                     LLLENKSLKGLDNIHIHGGNKTTLENKMLYKGIILIVDNDEILTGNLSEGSEAISIWT
                     KNSNILYIGVQYIKHEIYISEQIKGGYKNEQN"
     gene            3528..4409
                     /locus_tag="ACOIAF_003474"
     CDS             3528..4409
                     /locus_tag="ACOIAF_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HGZ2670454.1"
                     /translation="MNKIKIKQVDAFTTIPFGGNPAGVVTDASNISDEIKQNVAKEMN
                     LSETAFVSESNIADFKVQFFTPGLEVDLCGHATIGTFHALLEEGKLNTNKSIFYQETK
                     AGVLSVELKKINNENIFMMEQNTPEFENLDNCRKELAQMIGVHEDRLLEYPIIKVNTG
                     LWWLVFGLKSLDDLKNISPDLNKIKKFSGDNNIIGITPFCIETIDKKCDYHLRSIAPY
                     VGVTEDPVCGTGNACVSSYIVHHNILDKTSFIGEQGNFINRPGKVYVNITRENNDITS
                     VKIGGSAYTVLSGEIMY"
     gene            complement(4635..5990)
                     /locus_tag="ACOIAF_003475"
     CDS             complement(4635..5990)
                     /locus_tag="ACOIAF_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HGZ2670455.1"
                     /translation="MSSTVKKKGKFPMGFYICSTTFSFERAAYYASKYLIYIFLTTAI
                     VHGGLGIDKGQAAIMQANLVAFTYLAPIIGGYISDRWIGARYTIPIGMLIMGVGYYLG
                     SIATTVSMVNAMIILVSIGTAFFKGNVSAVNGQLFDSQEELDTAFSVQYSFVNIGSFI
                     GTIAVGILYLKTFAKNGVLGFSQCFFIAAVLCVIGAIWFIYGWRFLGNAGKRPFKEGV
                     VAEKVEEKDKSPLTSMDKKRIWAIILISFFSVIFWVFWYLTYLAVYDYGAAFVNMNVG
                     GFDVPLAWFDSLNSLVCIVLGPVLGALWFKLASRPQGDMSLFKKTGLGLIFLGLAFLM
                     LVGAEFSRGVGAPETAKASILWIIMFGILLSLGEMLFSPLGNSFVSKYAPKKLLGVLM
                     GVWTFATFIAGKGYGYIYAFTLKFDMIKVYTIIPVILFVAAILLFLSDKTLSRLVEDD
                     K"
BASE COUNT         2711 a          982 c          856 g         2512 t
ORIGIN      
        1 ttttatcata ttttcattga aatatattta attatttctt atgtatattt aattatttaa
       61 aatcaatcga cattttatat tacaaaaatt attttcaatt ttttccagta ttttcaagta
      121 tttcaactta attatatata tatttattga aattcagatt atagctttgt actaatcttt
      181 tctcaaatat atacattggt aatataattt ttaatttata taaattaaat taaaattttt
      241 tataattttt tttataaaat tatttgtatt atattttgta ttttttataa gattattata
      301 cttgtacatc ttttataaca tataaaatct ttttttctaa ttatatacaa tatattttcc
      361 attatgttct aatcaatttc tttactattt ttacaacttt attagtcata ttcataatta
      421 tttctaatta gtgtttaaca ttttatgtaa aatttataaa aaaatgtata taaattaaaa
      481 taagtgacta tactaaaaat atgatacgga taaacaataa gttcaaccta tctcccccaa
      541 aatcaagtga gctacagttt cccccaactg tagctcattt tcgtgtaatt taataaacat
      601 ctcaacaaga attaattaac aaaacccctt ataaaaacat aacatatatt aaacaagctc
      661 ttgattaaaa cttgagcttt tttaataaaa aacagaatag tttattagat aaggggtttt
      721 tttatgcaat tcatagatgt atctaaatac attgaaggtg caaaccttaa agttaaaact
      781 aaaaatggtg aaactgttaa aagaatatat tttaacaact ctgccactcc attagtttta
      841 aaaaacgtaa tggataactt aaactgtgag attccttggc ttacttatat taatgctcca
      901 ggaataatct cagaaaaaaa tactttaaaa tatgaaaatg tacgctctac tatcctcaaa
      961 cttattgatg gagacgaaga aaaggattct gttatttatg tcaaaaatgc tacagaagga
     1021 ataaacttct tagcaaaatt ttttaaagat gaaaatccag ataaatttgt tattaccaca
     1081 gcaatggagc atatggctaa ttacctgcct tttaaagtta attttcctac aaaagttgtt
     1141 ggaattacag aaaaaggaga acttgattta tgtcaattag agaatatact taaaaattat
     1201 gctggtaatg tatctctcgt tacagttaca ggagctagta atgtcacagg tataaccact
     1261 cctatatatg aaatagcaag aatagctcat aagtatggtg caaagatatt ggtggatatt
     1321 gtgcaagtaa ttcagcataa acctttttca atgatgccac atgaatgtga tgagcatata
     1381 gattttgttg tttttagtgc tcataaatgt tattctccac taaatggagg agctctggta
     1441 ggacctaaaa aattctttga aaaatttaat cctgtactat atggctcagg ttccaccaaa
     1501 tttgtaaatg ataacaaaat actatatgca gacatacccc aacgttttga agcaggatac
     1561 ccagagtatt ttggaactct tgcaatggga aaagcactga acactttaaa taaaattggt
     1621 ctaaacagaa tttcaaaata cgaaagagaa ttatttttat atgttaaaga agaaatgagt
     1681 aaaataccta atataataat atatggagat aaatcaaata atgtaattat cccaaatata
     1741 tcttttaata taagtaatat gtattacaaa gatgtagcaa aatatttagt tgataacttt
     1801 ggaatagaaa ctggagctgg agtagtaggt gcagatattt atgtacaaaa attattaggt
     1861 ataagtccaa aagaagcata tgtcagattt atgagggaaa atcctgtagg tcttgttagg
     1921 ataagcttag gaatgtacaa tacttttgat gaggtggata gctttataca tgctattaaa
     1981 actttggctt ttaaataagt tttttctatt cttttttatc aataaataga gagtagtttt
     2041 gttttcacaa aaaagcaaat tatatcaaca gtcaatttca ttagcaatta gacactcatt
     2101 tgtaactata caaactattt tacactatac aaacaaaaaa ctcattacaa tgagtttttt
     2161 tgtttatata tgagatttat tttaattact tcaatttact ttaattacat atatgattca
     2221 attttgtttt ttaaattctc cttaggcata aaacctatta atctatctac aggttttcca
     2281 tccttaaata ttagcatagt tggtactgta gatacttcaa atttctgagc taattctata
     2341 ctttggtcta tgtccacctt caagaagttt gctttttcaa ccatctcatt tcctaaagct
     2401 tcatatattg gtgatagcat cttacaaggt ccacaccaag tcgcaaagaa atctacaact
     2461 actaaaccat ctttattttc tacttcattt ataaattcat tattatttat tatttttacc
     2521 ataatataca tctcctttat tatcttcttg atattaatat actacgccct atataacagt
     2581 tcaattaggc acttttttgt aaccgcctat ttttaatagg tttaatatta tgataaatag
     2641 atttataaga ttaaatattt atgagactaa atatttctaa tatattttat ttttttctta
     2701 atatagttga cattttttcc cttaaattat aaaatcaaat taataactac ataggtagtt
     2761 actaatcttt tatcaaagga gtttctatat ggataatata attaatgaat tacaaaatat
     2821 aggatttact aaatacgaat ctcaaatata catatcatta ttaaaggaaa gccctttaac
     2881 aggatatgaa ataagtaaat tatcaggtgt accacaatca aaagtctatg aaaatataac
     2941 aaagctatta aacaataata taattatcaa tgttggaact gaccctatta agtacatacc
     3001 aataaaccct aatgaattat taaaaaataa gaaaaaagaa tttgacaatt ctatggataa
     3061 tctaaagaat agcttacaga tattaaataa aagtaaaagt gtagaatacg tattaaacat
     3121 aaaaggagat aaaaatatat taaaaaaagc aatagaaatg ataaaaaatg cgaaacaaga
     3181 aattataatt tcttgccctt atgaagaaat tcttgaatta aaaaaagaac tcctaattgc
     3241 ttacaaaaaa aatattaata ttgaaatact acttttagaa aacaaaagtc tgaaaggtct
     3301 tgataatatt catattcatg gaggtaataa aactacactt gaaaataaaa tgctttacaa
     3361 aggaattatt ttgattgtag ataatgatga aatattgaca ggaaacttat cagaaggcag
     3421 tgaggccatt tcaatttgga caaaaaactc taacatttta tatattggag ttcaatacat
     3481 aaaacatgaa atttatattt cagaacaaat aaaaggaggt tataaaaatg aacaaaatta
     3541 agattaaaca agttgatgct tttacaacta taccttttgg tggaaatcct gctggtgtcg
     3601 ttactgatgc ctctaacatt tctgatgaaa ttaagcagaa tgtggctaaa gaaatgaatt
     3661 tatcagaaac tgcatttgtt tcagagtcaa atattgcaga ttttaaagta caatttttca
     3721 caccaggttt agaagttgat ttatgtggtc atgcaacaat aggtactttc catgcacttt
     3781 tggaagaagg gaagcttaat actaataaga gtatatttta tcaagaaaca aaagctggtg
     3841 tattatctgt agagcttaag aaaataaata atgaaaatat atttatgatg gaacagaata
     3901 cacctgaatt tgaaaattta gataattgca gaaaagaact tgcacaaatg ataggtgtgc
     3961 atgaagatag acttcttgaa tatcctatta taaaggtaaa tactggactt tggtggcttg
     4021 tatttggtct aaaatcttta gatgatttaa aaaatatctc tccagattta aataaaatca
     4081 aaaaatttag tggtgataat aatataattg gtataactcc attttgtata gaaactatag
     4141 ataaaaagtg tgactaccat ttaagatcta tagcaccata tgttggagtt acagaagacc
     4201 ctgtatgtgg aactgggaac gcttgcgtat catcttatat agtacatcac aatattttag
     4261 ataaaactag ttttattggt gagcaaggta actttataaa cagacctgga aaggtatatg
     4321 taaatataac tagagaaaac aatgacataa cttctgtaaa aattggagga agtgcttata
     4381 ctgttttaag tggtgaaatt atgtattaat cccataccta ataaaaacaa ataaaaatac
     4441 tagctaacta aagctagtat ttttatttgt tatttttatt taataatcat actctaacaa
     4501 atattataat tatatatatc ataatattgt atttagaagt aaaattttat ttttattatt
     4561 aattagttat aaatcaatct aatacaacat aattacagta gattaatcta taaaacataa
     4621 taattactta ctgattattt atcgtcctca acaagtctac ttaatgtctt atcagacaag
     4681 aataataata tcgctgcaac aaatagtatt actggaatta tagtatatac tttaatcata
     4741 tcaaatttaa gagtaaatgc atatatatat ccgtatcctt taccagctat aaaagtagca
     4801 aatgtccata ctcccataag aacacctaga agcttcttag gtgcatactt acttacaaaa
     4861 gagttaccaa gtggtgagaa taacatttca ccaagactta aaagaatacc aaacatgatt
     4921 atccatagta tactagcttt agcagtttca ggagctccaa cccctcttga aaattctgca
     4981 ccaacaagca ttaagaatgc aagacctaag aatattaatc caagacctgt ctttttaaat
     5041 aaactcatat ctccttgtgg tctactagca agtttaaacc ataatgctcc aagaactggt
     5101 cctaatacta tacatacaag tgaatttaat gagtcaaacc aagcaagagg aacatcaaat
     5161 cctccaacat tcatgtttac aaatgctgca ccataatcat aaactgcaag ataagttaga
     5221 taccagaata cccagaaaat tacagagaag aatgatataa gtataatagc ccaaattctc
     5281 ttcttatcca tgcttgtaag aggagactta tctttttctt caactttttc agcaactaca
     5341 ccttctttaa aaggtctctt tcctgcattc ccaaggaatc tccatccata tatgaaccat
     5401 attgcaccta ttacacaaag tacagctgct ataaagaaac attgagaaaa tccaagtaca
     5461 ccattttttg caaatgtttt aaggtaaaga ataccaactg ctatagtacc aataaaagaa
     5521 cctatattaa caaaagaata ctgtactgaa aatgctgtat caagttcttc ttgactatca
     5581 aaaagctgtc catttaccgc agatacgtta cctttaaaga atgcagtacc aatcgaaact
     5641 aagattatca ttgcatttac catagaaact gtagttgcaa tagaaccaag atagtatcct
     5701 acccccatga taagcatacc tataggtata gtatatctag ctcctatcca tctatctgat
     5761 atatatcccc caattatagg tgcaaggtat gtgaatgcaa caaggttggc ttgcattata
     5821 gcagcttgtc ctttatctat tccaagacca ccatgaacta tagctgttgt aaggaatata
     5881 tatattaagt actttgaagc ataatatgct gctctttcaa atgagaaagt tgttgagcag
     5941 atataaaatc ccataggaaa tttacccttc ttttttactg ttgagctcat aattttaaat
     6001 cctttcttaa ttgtattttt attttttacg ttttgtccaa attctttctt ttatgaaaga
     6061 tataatatta ttattttgta aaacaatttg taattattat taagataatt gcgcatttat
     6121 ttgtagagta catattctca tggaataaaa ttaatacact tcttaatcat aaatttaaat
     6181 tatataaatt cactattttc agatatagtt ataaattaat atattctcat ctaataccat
     6241 gaaaaaacct tcgccatatg caattcttat tttttacgtg atagtatttt acaaactaaa
     6301 atcccccttt tgtagcaact tatgtaatta cattttagtg cttttttaaa ggagtctcga
     6361 ataaagcaac atatattttg ttcaaataat gtttttattt attttttaaa tttatatatt
     6421 acttcaccca accaattctc aacttaaata actaaactac tatctattca cagattaggc
     6481 ttatttctta cacattagtt tataataaac actgtgaatc taaacataaa aactgttatt
     6541 ttaaagtaat tacccatata ttcgcttcga tactatttat tgaggttata ctctttatgt
     6601 ataatatatt atttataaaa tacctcaaaa tatattattc ataaaatacc tcaaactgaa
     6661 aactttttca cgaatattta tttttcacct cccctttttt atttcaccat gtaaatcgta
     6721 ccataaatat gcacttatat gcaagaaaaa aatctattaa tcgacagtaa ataattaaaa
     6781 aaattcaaat tatatttatc aagtatatta tataaaatat ttcctatgaa aatataaatt
     6841 aaattaatgt tcagaatagt ttttagatat gaaatcttga tttttttatt actttactta
     6901 tttgctcaaa tatatttttg tatgcagtat ataataatca aattatacac ttcttataac
     6961 tgcattgatt tgaaaataat attaataatt aatagaataa aaaattaaat taaatttaaa
     7021 aaaatttgtt aagttgattg aatacttttt tatgtattta a
//
LOCUS       DBGJFF010000166         6982 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.170, whole genome shotgun sequence.
ACCESSION   DBGJFF010000166 DBGJFF010000000
VERSION     DBGJFF010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6982
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.170"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            62..2182
                     /locus_tag="ACOIAF_003476"
     CDS             62..2182
                     /locus_tag="ACOIAF_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HGZ2670456.1"
                     /translation="MKQMELAIVSLKKDAGEIYENQIRQFLGDNLKINLYSFEEGNLK
                     FFKEKLILLSAYLKYDEIVKLSHYDAQIIVPKLTFEKNSIDMISKLEKDKIIYVYNLS
                     KDMAIETISLIHRLGIDNINILPCYPEIEFTPTDAVILTPGEKILPKFKNCEVIDLKY
                     RIIDLSCIVEIATKTKLKHLIKDDLIKKYVEKIIPTSYSTGELLLDANKFERQFDLLL
                     SIIDDGIICTNNDGIIQFYNHMARKILSINANEMIDSFVGDCIKDINFQSILTNKTPF
                     FEKLIKINHIDINLEIKHIQLNVFDGFILKMTKFSQLEKKQAKLRAQLVNSGNISKYT
                     FDDILGSSIQTINTKKIANKMAQSNSSILIIGESGTGKELFAQSIHSASRRKDGPFVA
                     VNCSTFQENLLQSELFGYDEGAFTGAKKGGKIGLFELANNGTIFLDEIGEMDLNSQSK
                     LLRVIQEKQVRRIGSNNVIDIDVRIIAATNRNLKELVSKNMFRRDLYFRLNVLPLKIH
                     PLRERPADIFEIFGSLKYDIPCNFILSEEVKEIFKMYRWEGNVRELRNLGEYFCYLEK
                     DIIEICDLPEYILDTIDSNYSRTVCNKVSDNIKKYQFNIGKDKNIMKYDYNFKRSLDE
                     YIFILDNLKKAYDLKERIGRKSLCKIALEENRFLTEQQIRNMLLELQDFGLVDILVGR
                     GGSIITSKGVEFLKNINRSNKLNS"
     gene            2407..3573
                     /locus_tag="ACOIAF_003477"
     CDS             2407..3573
                     /locus_tag="ACOIAF_003477"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HGZ2670457.1"
                     /translation="MNYNFNEIVDRSNNFSSKWSEMEKKYGTNDLLPMWVADMDFKAT
                     PCIIDSLKNRLEQEIYGYTTRPDSYNESIVNWLDRRHNWKIKSEWLIYSPGVIPAISL
                     LINELTKANDKIMIQEPVYSPFNSVVKNNNRELIISPLQKLENGDYIMDYEDIENKIK
                     DVKLFILCNPHNPVGRVWTKDELKKLGDICLKHNVKIISDEIHSDIILKKHKHIPMAS
                     ISKEFEKNTITCMAPTKTFNIAGLQSSYVILPDEKDYKLLDDAFTRIDIKRNNCFSLV
                     ATEASYNNGESWLESFLEYLESNIDFAIKYINENMPKLKVRKPEGTYLLWVDFSALGL
                     SDEELESILVQKGKVALNQGNSFGIGGSGYQRINLACPRSMLEEALIRIKNAIN"
     gene            3598..5040
                     /gene="nhaC"
                     /locus_tag="ACOIAF_003478"
     CDS             3598..5040
                     /gene="nhaC"
                     /locus_tag="ACOIAF_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HGZ2670458.1"
                     /translation="MKMNTERKIPSFRYSLLVMLAIIALTSVGIVVFNASITTMFLLS
                     WLIVVPAAMKLGYTNDEIEAFGFEVGKDAFQSNLIILSVGVLIAAWIAAGTIPIVVYS
                     GLTIITPKYFLLTTLIVCSLTSVATGTSWGTLGTSGIAMMSIGTSMGIPLGLTAGAVI
                     SGAFFGDKMSPLSDSTNLAAAVCKTDVITHMKHMFYTTGPAYVICIVLYTVIGFKYSN
                     NTIDYVQINQIKDVLNSNFHIGLVAMIPIIFLLLLLLLQKPPIISILSSAIMGLIIAV
                     FQEGEKIGDVLNYMLSGFTIDTGFVYADKLLNRGGIMSMAETVLLVFVVFVIAGILQK
                     TGFLEVLLQPLITKIGKSRTKLVGSTFIVSYFANAFSSSMMFTSVFVGTLMSPIYKEF
                     KLKPQNLSRIIEDTATLGGPLIPWNSNAVFCAQTLGVSPLEFIPYCFLSWITPIISFI
                     YGVTGFTMLTYTDDEIEELEIFDTAQRRVD"
     gene            5043..5312
                     /locus_tag="ACOIAF_003479"
     CDS             5043..5312
                     /locus_tag="ACOIAF_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670459.1"
                     /translation="MKFEYYYLIQDIAGILLAFIGLRMSIIGFRILSMKGLSINTLLI
                     VIKYCLFTIAGLNLLISKFGIRHWIWSVCMLIISIIINPRIKVSK"
     gene            complement(5389..6816)
                     /locus_tag="ACOIAF_003480"
     CDS             complement(5389..6816)
                     /locus_tag="ACOIAF_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp14"
                     /protein_id="HGZ2670460.1"
                     /translation="MKLTKSISSMLCLSLVLSLSPVAYANEISRTTVENTLVGQDGYQ
                     TAVEVSKKGWSSANEAVVVNSESIVDALAVTPFAKLKNAPILLTEKNNLNSQTEKELK
                     RLGVKKVYIIGLYSSVSNDVQKQLESNNLTTDRVGGSDRYQTALSIAKKIEGLKDISE
                     IAVVNGYTGLADAVSIASVAATNGMVILPVSDASGISSFKDFIESKNINKSYIIGSTN
                     AVSDKIKQSLPNSERIGGADRNETNGKVIEKFYTSNKLNNVFVAKDGMKNQNQLIDAL
                     AVGVLASKENSPVLIVGEQLSATQKSVFSNKNATSITQVGSGGNENAFKELKDLQANS
                     NNKRVMYVTNTDKVNIRNDATIEASVIGYLNNGDEVEVLDVLKTGWVKIKYNEGIGYV
                     SGSYLTNNKPDNSNENIKIKYVKEKDGLNVRKGPSTEDEKIGQLSYGSKVETVEMFAT
                     GWVKIKYNGGYGYVSNDYLSDTPVI"
BASE COUNT         2539 a          944 c         1006 g         2493 t
ORIGIN      
        1 atattattta atttaattat tatgtaatct aaaataagtc aaatttgtat gagaggttaa
       61 aatgaaacaa atggaattag ctattgtaag tttaaaaaaa gacgctggag aaatttatga
      121 aaatcaaata aggcaattct taggtgataa tctaaagata aatttatact catttgaaga
      181 gggaaattta aagtttttta aagaaaaact tatactccta tctgcttatt taaaatatga
      241 tgaaatagta aaattatctc actatgatgc tcaaatcatt gttcccaagt tgacatttga
      301 aaagaacagt attgatatga tatccaaatt agaaaaagat aaaattattt atgtatataa
      361 cttaagtaaa gatatggcaa tagaaacaat ttctttaata catagattag gaatagacaa
      421 tataaatatc ctgccctgct atccagagat tgagtttact cccacagatg ctgtaatcct
      481 aactcctggc gaaaaaatct tgcctaagtt taaaaattgt gaagttatag atttaaaata
      541 cagaataata gatttaagtt gtattgttga aattgctaca aaaaccaaat taaaacatct
      601 aataaaagat gacctaataa aaaaatacgt agaaaaaata attcctacaa gttatagtac
      661 aggtgagtta ttgttagatg caaataagtt tgaaagacaa tttgatttgt tactatctat
      721 aattgatgat ggaattatat gcacaaataa cgatggaata attcaattct ataatcatat
      781 ggctagaaaa attttgtcta taaatgcaaa tgaaatgatt gactcatttg ttggtgattg
      841 cataaaagac atcaattttc aaagcatatt gaccaataaa actccatttt ttgagaaatt
      901 gattaaaata aatcatattg atataaattt agagattaaa catatacaat taaatgtatt
      961 tgatggattt atactaaaga tgacaaagtt ttctcaatta gaaaaaaagc aagctaaatt
     1021 aagagcacaa ctagtaaact ctggtaatat cagtaaatac acatttgatg atatattagg
     1081 ttctagtata caaactatta atacaaaaaa aattgctaat aaaatggctc aatcaaattc
     1141 atctatactt ataattggtg aaagtggtac tggtaaagaa ttatttgctc aatccattca
     1201 tagtgcttct aggagaaaag atggtccttt tgtcgcagta aattgtagta cttttcaaga
     1261 aaatctactg caaagtgaat tatttggcta tgatgaaggg gcttttactg gtgctaaaaa
     1321 aggtgggaaa attggtttat ttgaattagc caacaatgga actatatttt tagacgaaat
     1381 tggagaaatg gatttaaatt ctcaatctaa attacttaga gtcatacaag aaaaacaagt
     1441 acgacgtatt ggttctaata atgtcattga cattgatgta agaataattg cagctactaa
     1501 tagaaattta aaagaacttg tatctaaaaa tatgtttaga agagatttat attttaggtt
     1561 gaatgttctt ccccttaaaa tacatccact tagggaaaga cctgctgata tatttgaaat
     1621 ttttggttca ttaaaatatg atattccatg taattttatt ctaagtgagg aagtaaaaga
     1681 aatctttaaa atgtatagat gggaaggaaa tgtaagagaa ttaagaaact taggagaata
     1741 cttctgttac ttggaaaaag atattataga aatatgtgat ttacctgaat atatattaga
     1801 taccatagat tcaaattata gtagaaccgt ttgtaataaa gtaagtgata atataaaaaa
     1861 gtatcaattc aacattggca aagacaaaaa catcatgaaa tacgactata attttaaaag
     1921 gtcactagat gaatacatat ttatattgga taatttaaaa aaggcttatg atttaaaaga
     1981 aagaataggt agaaaaagct tatgtaaaat agctttagag gaaaatagat ttttgacaga
     2041 acaacagatt agaaatatgc ttttagaact acaagacttt gggcttgtag acatacttgt
     2101 tggtcgtgga ggttcaatta taacatcaaa aggagtagaa tttctaaaaa atataaatag
     2161 gtctaacaag ttaaacagtt aaacctattt aacctattta attacatttt catatattta
     2221 aaaatttatt agaaataacc ttttccacaa agttttaagt ataattaata tttttatctt
     2281 atctatgctc tgaaattact taatattaaa tattaacttg tgaataaata ttttttatta
     2341 tcaatattgg cacgttgttt gcttaatatt attaacataa aattaaaatt actggaggta
     2401 tttacaatga attataattt taatgagatt gttgatagaa gcaataattt ttcatctaaa
     2461 tggtcagaaa tggaaaaaaa gtatggaact aatgatttac ttcctatgtg ggtggctgac
     2521 atggatttta aggctacacc ttgtataatt gattccttaa aaaatagact agaacaagag
     2581 atttatggct acacaacaag accagattct tacaatgaat ctatagtaaa ctggctagat
     2641 aggagacata actggaaaat aaaaagtgag tggttaattt atagtccagg tgttatccca
     2701 gctataagtc ttttaataaa tgaactcaca aaagcaaatg ataaaataat gatacaagaa
     2761 ccagtttaca gcccttttaa tagtgttgta aaaaataaca atagagaact aattataagc
     2821 ccattacaaa aactagaaaa tggagactac ataatggatt atgaagacat tgaaaacaag
     2881 attaaagatg taaaattatt catactttgt aatcctcata atccagttgg tagagtatgg
     2941 acaaaagatg aattaaaaaa actaggagat atttgtctta agcataatgt aaaaatcata
     3001 tctgatgaaa tacatagtga cattatatta aaaaaacata agcatattcc tatggcatct
     3061 atttctaaag aatttgaaaa aaatacaatt acttgtatgg ctccaactaa aacttttaat
     3121 atagctggtc ttcaatcatc atatgtaata ctacctgatg aaaaagacta taaattgtta
     3181 gatgatgcct ttaccagaat tgatataaag agaaataatt gctttagctt agttgcaact
     3241 gaagcttcat ataataatgg tgaatcttgg ctagaatcat ttttagaata tttagaatct
     3301 aatatagatt ttgctataaa atatataaat gaaaatatgc caaaacttaa agttagaaaa
     3361 ccagaaggta cttatttatt atgggtagac tttagtgcac ttggattaag tgatgaggaa
     3421 ctagaaagta tacttgtaca aaaaggtaaa gttgctctaa atcaaggaaa cagctttggc
     3481 attggaggaa gtgggtatca aagaataaac ttagcttgtc caagaagtat gcttgaagaa
     3541 gctcttataa gaataaaaaa tgcaattaac taaaaatatt taaggagaat ttgcactatg
     3601 aaaatgaata cagaaagaaa aataccttca tttagatatt ccttgttagt aatgcttgca
     3661 ataatagctc taacatcagt tggtatagtt gtatttaatg cttctataac tacaatgttt
     3721 ttactttcat ggcttatagt agttcctgca gccatgaaac ttggttatac taatgatgaa
     3781 atagaagctt ttggatttga agttggtaaa gatgcctttc aatcaaactt aattatactt
     3841 tctgttggtg tactaatagc agcttggata gctgctggta caataccaat agtagtatat
     3901 agtggactaa caataataac ccctaaatat tttctgctta caacattaat cgtatgttca
     3961 ttaacttctg ttgctactgg tacatcttgg gggactcttg gaacttctgg tatagcaatg
     4021 atgagtattg gtactagtat gggtattcct cttggtttaa cagctggcgc agtcatttct
     4081 ggtgctttct ttggagataa gatgtcacct ctatcagatt ctacaaactt agctgcagca
     4141 gtttgtaaga cagatgtaat tacacatatg aagcatatgt tttataccac aggacctgca
     4201 tacgtaatat gtatagttct gtatacagta attggattta agtactcaaa taatactata
     4261 gactatgttc aaataaatca aatcaaggat gtattaaatt caaactttca tattggttta
     4321 gtagcaatga ttccaataat atttttatta ttacttttat tgctacaaaa gccacccatt
     4381 atatctatac taagttcagc tatcatggga cttattatag cagtatttca agaaggagaa
     4441 aaaataggtg atgttttaaa ttacatgcta tcaggattta ctatagatac tggatttgtc
     4501 tatgccgaca aactattaaa tcgtggtgga ataatgagta tggctgaaac cgttttacta
     4561 gtatttgtag ttttcgttat tgctggtatt ctccaaaaaa ctggattctt agaagtactg
     4621 cttcaacctc ttataactaa aataggaaaa tcaagaacta aattggttgg gtctactttt
     4681 atagtaagtt actttgcaaa tgcatttagt tcatcaatga tgttcacttc tgtatttgtc
     4741 ggaactctaa tgtctccaat atacaaagaa tttaaattaa aaccacaaaa tctatctaga
     4801 attatagaag atacagctac acttggtggt ccattaatac catggaattc aaatgctgtt
     4861 ttctgtgctc agactcttgg tgtaagccca ctagaattta ttccatattg tttcttaagt
     4921 tggattacac ctataatttc atttatttat ggtgtaactg gttttactat gcttacatac
     4981 acagatgatg aaattgaaga gttagaaata tttgatactg ctcagagaag agttgattga
     5041 atatgaaatt tgaatactat tacttaattc aggatattgc aggtatatta ttggcattta
     5101 taggacttag aatgtctatt attggattta ggatactttc tatgaagggg ctttctataa
     5161 atacactttt aattgtaatt aaatactgct tatttaccat agcaggactg aatttattaa
     5221 tatctaagtt tggaattaga cactggattt ggagtgtttg tatgttgata atatccataa
     5281 taataaatcc caggattaag gtatcaaaat agaaatagtt attagactta aatttttata
     5341 aaaaaactgc tttacaaatt aaagcagttt ttttattgtt acttataatt aaataactgg
     5401 tgtatcagat aagtaatcat tacttacata accatatcca ccattatact ttatttttac
     5461 ccatccagta gcaaacattt ctactgtttc tactttgctt ccatatgaaa gttgacctat
     5521 tttttcatct tctgtacttg gtcctttcct aacatttaga ccatcttttt cttttacata
     5581 ctttattttt atattttcat tactattatc aggcttatta tttgtcaaat aagacccact
     5641 aacatatcca ataccttcat tgtattttat ttttacccat ccagttttta acacatctaa
     5701 aacttctact tcatcaccat tatttagata tcctataaca gaagcttcta ttgtagcatc
     5761 atttcttata tttaccttat ctgtattagt tacatacatt actctcttat tgttactatt
     5821 tgcctgtaaa tcttttaatt ctttaaatgc attttcattt cctccactac caacttgagt
     5881 tatagaggta gcatttttat tcgaaaatac actcttttgt gttgcactta attgttctcc
     5941 tacgattagt acaggagaat tttcttttga tgctagtact cctactgcta gagcgtctat
     6001 aagttgattt tgatttttca ttccatcctt agccacaaat acattgttta atttattaga
     6061 tgtataaaac ttttctatta ctttaccatt agtttcattt ctatctgctc cacctattct
     6121 ttctgaatta ggtaaacttt gttttatttt atctgataca gcattagtag agcctattat
     6181 gtatgattta tttatattct tagattctat aaaatcttta aagctagata taccagatgc
     6241 atcagatact ggaagtataa ccattccatt agtagcagct acagaagcta tactaactgc
     6301 atctgcaaga cctgtgtacc catttactac agctatttct gatatatctt ttaatccttc
     6361 tattttttta gctatactca aagctgtttg gtatctatca ctaccaccaa ctctatcagt
     6421 agttaaatta ttagattcta actgtttttg cacatcattt gatacagaac tatatagacc
     6481 aattatataa acttttttaa ctcctaatct ttttaattct ttctcagttt gtgaatttag
     6541 attatttttt tctgtcaata atataggagc attttttaat ttagcaaatg gtgttacagc
     6601 taatgcatct acaatagatt cactatttac aactacagcc tcatttgcac tactccatcc
     6661 ttttttactt acttcaacag cagtttgata tccatcctga cctactaaag tattctcaac
     6721 tgttgttcta cttatttcat ttgcatatgc tactggtgat aagcttaaaa ctagagataa
     6781 gcacagcatt gatgatattg atttagttaa tttcatcttg actcccccta aaattatagt
     6841 atctaaatgt ttcctattta aaatagtata acataataat ttaagtaaca tcattattat
     6901 aaaatattat ttttcttatc taataagctt ttattcttac ttgatattaa tactttgctc
     6961 atcaaatgaa ttttttatat aa
//
LOCUS       DBGJFF010000167         6962 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.171, whole genome shotgun sequence.
ACCESSION   DBGJFF010000167 DBGJFF010000000
VERSION     DBGJFF010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6962
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.171"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            21..1424
                     /locus_tag="ACOIAF_003481"
     CDS             21..1424
                     /locus_tag="ACOIAF_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HGZ2670461.1"
                     /translation="MHKPSYKTVFHSYIIFFMIFICLILTVIGIFLSLIIIQKPNGEF
                     IRSDWPKKFTENFNEQIVFIDNKPQVKQSGLEKLRENQLWLQILDSSGNRVYGFLEPK
                     EHKDYYSNSELLALANQNNSSNGTVYFNTIKNNKNDYIYIIYFPLNISKVNMFFDGGK
                     FIGGKSIVFSIIGIIFLIILVSGIAYGYWVTKCISNITSAVSDISKRSYLPVNIKGVF
                     ADIYNSLNILNEEIHKSDEIQNKTDIMREEWISNITHDLKTPLSPIKGYAELLIDSST
                     NYSPEKVYKYASIMLKNIEYTNTLINDLKLTYQLKNGIIPLNKNNYDLVRFLKELSID
                     ILNYPEYESNIVEFKSNIDNINFDFDCTLLKRAFSNLIVNAFTYGDKNTKVCVHVTLN
                     ERIEIIISDNGKGMSREEASNLFNRYYRGSNTKQKIEGTGLGLAITKQIIEVHGGNIS
                     VESIPGLGSSFYITFPI"
     gene            1643..4120
                     /locus_tag="ACOIAF_003482"
     CDS             1643..4120
                     /locus_tag="ACOIAF_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HGZ2670462.1"
                     /translation="MKIILKYIFTNIKERKIRTAVMLLSIVLSTVLLFVSFSIGLSYE
                     SAQRKMAKGMYGTATISVQSKNPDILTNLEDIPDLNAIKSKVGVLESSAIYNKGGYYE
                     EFSLISADLSQLNKINKPRLENGDSITDFSGDKIILPNRFTSKYKIKKGDSITLQIYG
                     KSYTFQVSDIASYDTVFLRNTRGVNALLPKETLSKIINKGSGYTRVLIESEEEMTENL
                     VNKLSEELSTEKYMVSNTINETKIISDARQKTMPFFLISFFALTLSIFIIYSSYKVIT
                     LERLPFIGTFRSIGANEKTVTRILMLESILYGSIGGLIAIPIGVVVLNLMLHGLGNSL
                     EQGISIPVVISPIGVIISVIVAIIVSSFSAYIPVKKASHLPIKNIVLGTVEEKNVSNR
                     SILFIGSIMFILSILLPRISPENTLYLAGGFSLLGLIVATIVLIPLITDIMSIVFEFV
                     YKNILGNEGKLAARNMKNNKNIIQNITLLFISISAVIAISVVGNFVKTYITDVFRDAE
                     LQGFADGKMNEEFIEDVRHMDGIKKILPLYVMNNEISGNGVTLSRLEGTDNIKLYNSM
                     FGINYTNFEIKKQVIEAFNDKRSIILNEDTLKKVGLSIGDTITLSNDKYDFSYKIVGS
                     FKSRANDVEAVIPSHYAVNDFDKTNYGFLVYTAVNPDAVMIQIRYLFGDTYNWSRTVE
                     EFNNDSLNTISSFLSPMNKMTYFIFLLATVGIINNLLINYIQKRRSIAMYKSIGLSNK
                     QNIKVTLIEGFTSGLLGAVIGIVISILEIQTIFIVAGPKISMKPDLDFKTFIIVGLLG
                     IIVTLIGSIVPIIKGKKMKLIEEIKFE"
     gene            4135..4821
                     /locus_tag="ACOIAF_003483"
     CDS             4135..4821
                     /locus_tag="ACOIAF_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2670463.1"
                     /translation="MKITHNSLAVEAKNIIKEYKIGNTTTRVLKEVSLQVMKGEFVSI
                     MGQSGSGKSTLLYILGGLDTPTSGKVYMNGADISHFNDEKMSIIRRRNIGFVFQFYNL
                     IPNLNVEENIMLPLLLDGKNLKDYKNQLDEILDIVGLTDRCKHTPRELSGGQQQRVAI
                     ARALIGKPEILFADEPTGNLDSKTGIEIIDLLNKINRDNGQTIIMVTHSPEAAKSSSR
                     TITVSDGLIV"
     gene            5095..5928
                     /locus_tag="ACOIAF_003484"
     CDS             5095..5928
                     /locus_tag="ACOIAF_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGZ2670464.1"
                     /translation="MLKKESIHFINKNRSLIVCEDNFVYLLPCIQLRNWISNFTISFP
                     NQTIISDNYTIIPHGSVTLVFFYDATGLHSLLFGPTTKPKTVGNIANRCDVILIIEFQ
                     PAGFFPLIGIQQSELIDKVVPFSIINTSLDLEIKKIFNESLSIDKLILKLEELLISNI
                     KIEYSYEFILAIQLIIQNSGNISSQEISKKVFYSSRHLNRLFNQYLGLSMKSFSRLVR
                     INKSIKLLNNNKTSLMSICNELGFYDIPHFIKDFKIVCGITPQKYRANMSDFYSEIAK
                     F"
     gene            5966..6427
                     /locus_tag="ACOIAF_003485"
     CDS             5966..6427
                     /locus_tag="ACOIAF_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGZ2670465.1"
                     /translation="MKFGGVLLIVKDINKSKKFYEDVMEQKITLDLGEHVSFENGFGL
                     QSNYKAIIGYDLPIYQQSNNFQLYFEVNDIKQWESKINRIGNIEFLHYIKEYPWGQRT
                     FRFYDFDKNIIEISESMESVIKRFLKQGLALEEISKRTMYPVEFITQFQQL"
     gene            6458..6706
                     /locus_tag="ACOIAF_003486"
     CDS             6458..6706
                     /locus_tag="ACOIAF_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGZ2670466.1"
                     /translation="MQKTKAWGIIEPEKPESQQQMAHMDFAVNDLEEAVQYAIHCGAT
                     IAEEQFTDDWRVMIDPAGHPFCLCQMKSIMESSHFSLL"
BASE COUNT         2685 a          742 c         1080 g         2455 t
ORIGIN      
        1 ttaagaaaag agttgattaa atgcataaac ctagttataa aacagtattt cattcataca
       61 ttattttttt catgattttt atttgcttaa tcctgacagt aataggtatt ttcttatcac
      121 ttattattat tcagaaacca aatggggaat ttattcgtag tgactggccg aaaaaattta
      181 cagaaaactt taatgaacaa atcgtattta tagataataa gccacaagta aaacagtctg
      241 gattagaaaa acttcgagaa aatcaattgt ggttgcaaat tttagattct agtggaaata
      301 gagtttatgg gtttttagaa ccaaaagagc ataaagatta ttattctaat tctgaattat
      361 tagcattagc taaccaaaat aattcaagta atggaactgt atactttaat acaattaaaa
      421 ataataaaaa tgactatatt tatattatat attttccatt aaatatatct aaagtgaata
      481 tgttttttga tggaggtaaa tttataggtg gtaagtcaat tgtattttca atcataggga
      541 ttatattttt aataattttg gtttctggaa ttgcttatgg ttactgggta acaaagtgca
      601 tatcaaatat tacatctgct gttagcgata tttcaaaacg aagctatctt ccagttaata
      661 ttaaaggtgt ttttgctgat atatataata gtttaaatat tttaaatgaa gaaattcata
      721 aaagtgatga gatacaaaat aaaactgata ttatgagaga agagtggatt tcaaatataa
      781 ctcatgattt aaaaactcca ctttcaccaa taaaagggta tgcagagctt cttatagata
      841 gtagtactaa ttattcacct gaaaaagtat ataaatatgc ttcaatcatg ttaaagaata
      901 tagaatatac aaatacactt ataaacgatt taaaattaac ctatcaatta aaaaatggaa
      961 taatcccttt aaataaaaat aattatgatt tagtaagatt cttgaaagaa ttatccattg
     1021 atattttaaa ttatccagaa tatgaatcaa atatagttga atttaagagt aatatagata
     1081 atattaactt tgattttgat tgtacactat taaaacgagc ttttagtaat cttattgtca
     1141 atgcttttac atatggagat aaaaatacta aagtttgtgt acatgtaaca ttaaatgaaa
     1201 gaattgaaat cattatatct gataatggaa aagggatgtc aagagaagaa gcatcaaatt
     1261 tgtttaatcg ttattataga ggaagcaata caaaacaaaa aatagaagga actggtttag
     1321 gactagcaat aacgaagcaa attattgaag ttcatggagg gaatatatct gttgaaagta
     1381 tacctggatt aggtagctca ttttatatca cctttcctat ttaattaagg ttaattaagg
     1441 ttaaataaaa aatatattaa ggttgataga ttattatatt tatatagtag tttatcaatc
     1501 ttttttattt aaggggtgat ttgattaaaa atattataaa tagaatgata aaagtatgga
     1561 ggagtctatt agagatttaa tttagattaa agtataaaaa tattagattg aaatatctta
     1621 gataaggaaa gaaggtgtaa atttgaaaat tatattaaag tatattttca caaatattaa
     1681 agaacgaaaa attagaacag cagttatgtt attgtccata gttttatcaa cagttttact
     1741 ttttgtttca ttctcaattg gattatctta tgaaagtgca caaagaaaaa tggcaaaagg
     1801 tatgtatggt acagcgacta tatctgtcca aagtaaaaat ccagatatat taaccaattt
     1861 agaagacata cctgatttaa atgctataaa atcaaaggtt ggtgtattgg aaagctctgc
     1921 tatttataat aaagggggat actatgaaga atttagtctt atatcagcag acttaagtca
     1981 actaaataaa atcaacaagc cacgattaga aaatggcgat agtattactg atttttctgg
     2041 tgataaaatt attcttccaa atcgctttac atcaaaatat aaaataaaaa aaggtgattc
     2101 tattactttg cagatttatg gaaagtctta tacttttcaa gttagtgata ttgcatctta
     2161 tgatacagta tttttgagga acacaagagg tgtaaatgca ctgctaccca aagagactct
     2221 atcaaaaatt ataaataaag gcagtggata tacaagagtt cttatagagt cagaagaaga
     2281 aatgacagaa aatctagtaa ataaattatc cgaagaactt tctactgaaa aatatatggt
     2341 atcaaatact ataaatgaaa ctaagataat ttcagatgct agacaaaaga caatgccttt
     2401 ctttttaata agtttttttg cattaaccct tagtatattt ataatataca gcagttataa
     2461 ggttattaca ttagaacgtc ttccttttat tggaacattt agaagtattg gtgccaatga
     2521 aaaaactgta actcgtatat tgatgcttga aagtatatta tacggaagta taggaggatt
     2581 aattgctatt cctattggtg tagttgtttt aaatttaatg cttcatgggt taggaaattc
     2641 tctcgaacaa ggaatatcaa ttccagtagt aatatcaccc attggagtga ttatatctgt
     2701 tatagtagca ataatcgttt cttcatttag tgcatatata cctgttaaaa aagctagcca
     2761 tttgccaata aaaaatattg tactaggaac agtagaagaa aagaatgttt caaatcgttc
     2821 tatacttttt ataggaagta taatgtttat tttatctatt ttgcttccaa gaatatcccc
     2881 tgaaaataca ttgtatctag ctggtggatt ttcattactg ggattaattg tagctactat
     2941 tgtgttgatt cctcttatta ctgatatcat gtctatagtt tttgagtttg tttataaaaa
     3001 tattttaggg aatgagggta aattagctgc tagaaatatg aaaaataaca aaaatataat
     3061 acagaatatc actttactat ttataagtat ttctgcagtt attgcaataa gtgttgttgg
     3121 taactttgtt aaaacttata taacagatgt gtttcgtgac gcagaattac aaggttttgc
     3181 agatggaaag atgaatgaag agtttattga agatgttaga catatggatg gaattaaaaa
     3241 aatattacct ctttatgtta tgaataatga gatttctggt aatggtgtaa cattatcaag
     3301 actagagggt acagataata taaaactata taactctatg tttggaatca attatacaaa
     3361 ttttgagata aaaaaacaag taatagaagc ttttaatgat aaacgttcta ttattttgaa
     3421 tgaagatact ctaaaaaaag taggcttatc tataggagat acaataactc tatctaatga
     3481 taagtatgat ttttcttata aaatagtagg tagttttaaa tcccgtgcaa atgatgtaga
     3541 agctgttata ccatcacatt atgcagttaa tgattttgat aaaactaatt atggtttttt
     3601 agtatataca gcagtcaatc cagatgctgt tatgattcag ataagatatt tatttggaga
     3661 tacatataat tggagtcgta cagttgaaga atttaataat gattctttaa atactataag
     3721 tagttttttg tctcctatga ataagatgac ctatttcatt tttcttcttg ctacagtagg
     3781 tattattaat aatctactga ttaattatat tcaaaaaaga cgttctatag ctatgtataa
     3841 atctatagga ctaagtaata aacaaaatat aaaagtaaca ttgattgaag ggtttacttc
     3901 aggtttactt ggagctgtta ttggtattgt gatatctata cttgagattc aaactatctt
     3961 tatagttgca ggaccaaaaa tttcaatgaa accagattta gattttaaaa catttattat
     4021 tgttgggtta ttgggaatta tagtaacttt aataggttcg atagtgccta ttataaaagg
     4081 gaagaaaatg aaactgatag aagaaattaa gtttgaataa ttatggaggt taatatgaaa
     4141 attacacata atagtttagc tgtagaagca aaaaatatta tcaaagaata taaaattgga
     4201 aatacaacaa ctcgagtgtt aaaagaggta tctttacaag taatgaaagg tgaatttgtt
     4261 tctatcatgg gtcaatctgg ttctggtaaa agtactcttt tatatatttt aggtggttta
     4321 gatactccaa cgagcggaaa agtgtatatg aatggagctg atatatcaca ttttaatgat
     4381 gagaaaatga gcataatacg tcgtagaaat ataggattcg tatttcaatt ttataactta
     4441 ataccaaatt taaatgtgga ggaaaatata atgcttcctt tgttattaga tggtaaaaat
     4501 ttaaaagatt ataaaaatca attagatgaa atcttagata tagttggttt aactgataga
     4561 tgtaaacata caccacgaga gttatctggt ggtcagcaac aacgtgttgc tatagctcga
     4621 gctttaattg gaaaaccaga gattttattt gctgatgagc ctactggaaa tttagatagt
     4681 aaaacaggga tagaaataat agatttactg aataaaataa acagagacaa tggacaaact
     4741 ataattatgg taactcactc accagaagca gccaaaagta gtagtcgtac tattacagtt
     4801 tcagatggtt taattgtata aggactaatt attgtatttg agataagcat ttttaactat
     4861 ataaattaga catatgtaaa gtggatataa gttaaatact ataaatgtgc tataatatta
     4921 aaataacaga taaaagtact atatttttta ttgattattt taattttatt tgtttattaa
     4981 atatctaatg agtattcata ctatctctat tattaaaagc agttaatgat atatagagat
     5041 aatatggatt cttcttactt taataaatat tcacgatagg acaggatata atatatgtta
     5101 aaaaaagaaa gtattcattt tataaataaa aataggtcac ttatagtttg tgaagacaat
     5161 tttgtttatc tcttgccttg tatacaatta agaaattgga tatcgaattt tactatatcg
     5221 tttccaaatc aaacaataat ttctgataat tacacgatta ttcctcatgg aagtgtaact
     5281 ttggtgtttt tttatgatgc cacagggtta catagtctat tatttggtcc aactactaaa
     5341 ccaaaaacag taggaaatat tgctaataga tgtgatgtta tactaattat agaatttcaa
     5401 cctgctggtt ttttcccgct tataggtata cagcaaagtg aacttattga taaagttgtt
     5461 ccattttcta taattaatac ttcattagat ttagaaatta aaaaaatatt taatgaatct
     5521 ttatctatag ataaattgat tttaaagttg gaagaattat tgatttctaa tattaaaatt
     5581 gaatactcat atgagtttat tttggcaatt cagttgatta ttcaaaactc tggaaatata
     5641 tcttcacaag aaatttctaa aaaagtattt tatagttcta ggcatttaaa cagactcttt
     5701 aatcagtacc tagggttgag tatgaagtca ttttctcgtt tggtacgtat taataaatct
     5761 ataaaacttc taaacaataa taaaacttca ttgatgtcta tatgtaatga attaggattc
     5821 tatgatatac cacattttat taaagatttt aaaattgtct gtggaataac tcctcaaaaa
     5881 taccgagcta atatgtctga tttttacagt gaaattgcaa aattttaagt tataatactt
     5941 ataacaaaga aaggatagtg ataagatgaa atttggagga gtattgctaa ttgtcaaaga
     6001 cataaataaa tcaaaaaagt tttatgaaga tgttatggag caaaaaatca cattagattt
     6061 aggagaacat gtatcatttg aaaatggatt tggattacaa agtaattata aagctataat
     6121 tggatatgat ttaccaatat atcaacaatc aaataacttt cagttgtatt ttgaggttaa
     6181 tgacatcaaa caatgggaaa gtaagataaa tagaatagga aatattgaat ttttgcatta
     6241 tataaaagaa tacccatggg gtcaaagaac ttttaggttt tatgattttg ataaaaatat
     6301 tatagaaatt tcagaaagta tggaaagcgt cataaagagg tttttaaaac aaggtttagc
     6361 tttagaagaa atttcaaaac gcactatgta tccagttgaa tttattacac aatttcaaca
     6421 attataaaat attaaaatat ataatttttt ctttatattg caaaaaacaa aggcttgggg
     6481 cataatagaa ccagaaaaac ctgaatctca acagcaaatg gctcatatgg atttcgctgt
     6541 taatgattta gaagaagcag ttcaatatgc aattcattgt ggagctacaa tcgcagagga
     6601 gcaatttact gatgactgga gagttatgat tgacccagct ggacaccctt tttgcttatg
     6661 tcagatgaaa tcaattatgg aaagtagcca tttttcattg ttatagacca aaatattgtt
     6721 tattaattac aaaaaagcac aacagtatat accgttgtgc ttttacttat tatacatgaa
     6781 tacactaaaa aatataaata gtagagcttt atcaataatg ataattttta aaaaatagta
     6841 tttttttatt gaataatttc ttagatattt taaagtatat aaattttgca tatatgaatt
     6901 ggacattatc aaatgtgtta taatattaaa acaacaaata aaattattat attttttaga
     6961 at
//
LOCUS       DBGJFF010000168         6900 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.172, whole genome shotgun sequence.
ACCESSION   DBGJFF010000168 DBGJFF010000000
VERSION     DBGJFF010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6900
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.172"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(493..1389)
                     /locus_tag="ACOIAF_003487"
     CDS             complement(493..1389)
                     /locus_tag="ACOIAF_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HGZ2670467.1"
                     /translation="MKDKNTYFNRKGQENFIDIFLKDDIKKGANFKRHWHEHLQIYYF
                     IDGIAYLECGLDNFKVVPGNIVIANSNELHYLESLSDNLKFYSIRIEPTFLFSNQVDL
                     LQTKYLAPLALNHIVFNNLIQNDSLILKCITTILDEYHNKEVGYELSIKANTYQLIVL
                     LLRSHIDKIYSEDEFIKRKYSLEKFECIFDLIEEKYMDKITLKELASRVNLSAHHFCR
                     TFKQITGKTTIDFINGIRLDKAIYYLKETDLNMTEIAMRCGFDSINYFSRLFKRNYNI
                     SPTKYRNEHREVSNVILKEDMK"
     gene            1531..2958
                     /locus_tag="ACOIAF_003488"
     CDS             1531..2958
                     /locus_tag="ACOIAF_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGZ2670468.1"
                     /translation="MTNSFKKDDNRKKITVLFVVIAMTFMATLDSSIINVALPVLASK
                     LNVSLASIEWVIASYSIIICSTLLFFGRLGDIIGKSRVFQVGTILFTSASLLCGLSNS
                     LTLLIVCRFIQGIGASAYMANNHGIITELFPKESRGKALGILVTAVAIGNMVGPSVGG
                     FILSIFDWNVIFFINIPIGLIVIFLNTKFLPNSKKSSENMDKTGAILQFLGTTLFFSA
                     LISAQQTGLLNPYILIALLLSIIFIILFLILEKKHPQPLLDLEIFRNFKFSLNLICAL
                     TSFICIASSSILIPFYLQSTMKLPPIQAGLFMILSPLILAIFSPIFGNISDKIKSEKI
                     ILIGLLVMSFGFFLMSRLKESSALILFVIYILIISIGQAIFQPANNALIMSSCSRSKL
                     GVVGSINSLVRNLGQVIGITISTTLLYNFMSIKAGYRVNDYVINNDKIFVFGMRNVYI
                     IVTLVCLIGAILIGFYLFKYNKNEQ"
     gene            complement(3154..5748)
                     /gene="clpB"
                     /locus_tag="ACOIAF_003489"
     CDS             complement(3154..5748)
                     /gene="clpB"
                     /locus_tag="ACOIAF_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HGZ2670469.1"
                     /translation="MDVEKMTLRVQKSLNEAYNEAVKNHNQQVDVIHLFSALINQEDG
                     LIPNIIEKMNISIDSLRNTVNFEIDKLPKVYGEGADSQGVSATRKINEVLIKAESISK
                     EFKDSYISVEHVMLAMMETESKSAVGKILKQYNINKNDFLNILSQVRGSQRVETQDPE
                     GTYDALARYGTNLVDLAKKNKLDPVIGRDEEIRRIIRILSRRTKNNPVLIGEPGVGKT
                     AIVEGLAERIVRGDVPEGLKDKIVFSLDMGALIAGAKYRGEFEERLKAVLKEVQSSDG
                     KIILFIDEIHTIVGAGKTEGSMDAGNLIKPMLARGELNCIGATTFDEYRKYIEKDKAL
                     ERRFQPVIAEEPTVEDTISILRGLKERFEIHHGVRIHDNAIVAAAKLSHRYIQDRFLP
                     DKAIDLIDEAGAMIRSEIDSLPTELDVVRRKLFTLETEREALLKENDDKSKSRLDDIQ
                     KEIAELKSKNDEMTAKYEKEKSQILDIKNLKAQLDEAKGKAEKYEREYDFNKAAEVKY
                     GEIPKLEEQIKQYEENMNDGSENSLLKEEVTEEEISSIVSKWTGIPVTKLVEGEREKL
                     LKLEDELHKRVIGQDEAVTAVSNAVIRARAGLKDERKPIGSFIFLGPTGVGKTELAKT
                     LARNLFDSEDNIIRIDMSEYMEKHAVSRLVGPPPGYVGYEEGGQLTEAVRRAPYSVIL
                     FDEIEKAHEDVFNMFLQILDDGRLTDNKGKTVDFKNTLIIMTSNIGSNYLLEAGGNIT
                     ETTNNLVMNEMKHRFKPEFLNRVDDIIMFKPLDQENIKKIIDIFMKDLKNRLKEKDIT
                     IEVTNSAKDVMVREGYDPVYGARPLKRYIGNTLETIIAKKLIAGDVYNGCTIVIDGKD
                     ENIEVLVK"
     gene            complement(5785..6381)
                     /locus_tag="ACOIAF_003490"
     CDS             complement(5785..6381)
                     /locus_tag="ACOIAF_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670470.1"
                     /translation="MSLKLDRNVLQWFDYVFENEKTSLRHYNFNCTLKEISSTSLNKV
                     AFILEKNNSKYWKLYFEIPAEVTLKLKQNIHPLFREYIYEQISLYNNNQIYNFVNSNI
                     LKVFNNIAIYQYNILENLYTIDFKKSFIDKCQYLLIGEKRLIDEDLYLIAKSKEVFDF
                     FNSDGTFNLTLSFDIQKNENLLDSLLELRKSIIINERI"
     gene            complement(6398..6802)
                     /locus_tag="ACOIAF_003491"
     CDS             complement(6398..6802)
                     /locus_tag="ACOIAF_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="HGZ2670471.1"
                     /translation="MDKTLKKDLNQEYTESKSLIMNKSKYMNVCFKNYIKVVEGMYTK
                     RDLIPLKLINSMDKNNKIRKTLYELSCEFEYPKTSLSSLFTELKKMDFLQRVKNGEYM
                     INPHIAYKGSRADKESLLAEYNQIKRPNKVSK"
BASE COUNT         2211 a         1105 c          721 g         2863 t
ORIGIN      
        1 aataaaaaac tcgtaataat aacaaaaaaa tctattattg tagaatcttg tatgtttttt
       61 ctatttttat atatttttca ggatgatttt tattataaaa ttttttataa atatataatt
      121 tttttatatt tatttgactt tcttttaatt ctttaggatg gatttaaatt ttctataaaa
      181 atcatactat tttaactgtt tacttgctta tttgatagtt ttatttcttt gattaaatat
      241 ctttcagaat ctaagccaga tgtctcaaaa aaattgattt ataatctaat cattttttct
      301 gctattctta tttttccagt tttagtttca tattaaaata tacttttcat agacactaca
      361 ttctttcata ctcattttca catttaacta tacaagtagt cttactcatt tatgtactat
      421 actttattaa tatttattaa gaaaaactca aagatttaaa aatatatatt ttctataaag
      481 tatatttatg aattatttca tatcttcttt taaaataaca ttactaactt ctctatgttc
      541 atttctatac ttagttggag atatattata attcctttta aataagcgac taaaataatt
      601 tatgctatca aagccacatc tcatagcaat ttctgtcata tttaaatctg tttcctttaa
      661 ataatatata gctttatcaa gcctgattcc atttataaaa tcgattgtag tcttacctgt
      721 tatttgctta aatgttctac aaaagtgatg tgcactcaag ttcacacgac tagcaagttc
      781 ttttaatgta attttatcca tatatttttc ttctatcaaa tcaaagatac attcaaactt
      841 ttctaaagaa tactttcttt ttataaattc atcttcagaa tatattttat caatatgact
      901 tcttaataag agtactatta attgataggt attagctttt atagataact catatccaac
      961 ttctttattg tgatattcat caagtatagt tgtaatacat tttaaaatca gagaatcatt
     1021 ttgaattaaa ttattaaata caatatgatt taaagccaat ggtgctaagt acttggtttg
     1081 taacaaatcg acttgattac taaaaaggaa tgtgggttca atacgaatac tatagaattt
     1141 taaattatct gataagcttt ctaaataatg taattcatta ctgtttgcta ttacaatatt
     1201 tccaggcact accttaaaat tatccaatcc acattcaaga tatgctatac catcaataaa
     1261 ataatatatt tgtagatgct catgccaatg tcttttaaag tttgctcctt ttttaatgtc
     1321 atcctttaaa aaaatatcta taaagttttc ttgacctttc ctattaaaat atgtattttt
     1381 atccttcatt attcccccct catcaaaata gtgctaatta taagcaatat agttattgta
     1441 ttcattattt catatcgcta ttatataaaa tgtaactatc attgtaaaca ttaacataat
     1501 gccagtcatt ataataagga ggtaataaaa atgacaaact cttttaaaaa agatgataat
     1561 agaaaaaaaa tcacagtttt atttgtagtc attgccatga cttttatggc aacattagat
     1621 tctagtatta taaatgttgc tctcccagtt cttgcaagca aactaaatgt atctctagca
     1681 tctattgaat gggtaatagc aagttactca attattattt gttcaacact attatttttt
     1741 ggaagacttg gagatattat tggtaaatca agagtatttc aagtaggtac tattctcttt
     1801 acatcagctt cattactatg tggattaagt aattctttaa ctttacttat agtttgccgc
     1861 tttattcaag gaataggcgc ttctgcctac atggcaaaca atcatggaat tatcacagaa
     1921 ctatttccaa aagaaagcag aggaaaagcc ctagggattt tagttactgc agttgctatt
     1981 ggaaatatgg ttggaccatc tgtgggagga tttattttgt caatatttga ttggaatgtt
     2041 atatttttta taaatattcc aatcggtctt attgtaatct ttttaaatac aaaatttctt
     2101 cctaatagta aaaaatcttc agaaaatatg gataaaactg gtgctatact tcaattttta
     2161 ggaacaacat tattttttag tgctctaatt agtgctcaac aaacaggatt actaaatcca
     2221 tatatattaa ttgcactatt attatcaatt atttttatta tattattttt gatattagaa
     2281 aaaaaacatc ctcaaccgct tcttgacctt gaaattttta gaaattttaa attttcattg
     2341 aatttaattt gtgctcttac atcattcatt tgtattgcat catcaagtat tttaattcca
     2401 ttttatttac aaagtactat gaaacttccc cctattcaag caggtttatt tatgatacta
     2461 tcacctttga ttttagccat tttttcacct atatttggga atatatcaga taaaattaag
     2521 tcagaaaaaa ttattttaat tggtcttctc gttatgagtt ttggattctt tctaatgtca
     2581 agattgaaag aaagttctgc ccttatttta tttgtaattt atatattgat aatatcaata
     2641 ggacaagcta tatttcaacc tgctaacaat gctttgataa tgtcaagctg ttctagaagt
     2701 aagctaggag ttgtaggaag cataaattcc cttgtaagaa atttaggaca agttataggt
     2761 attactattt ctacaacact tttatataat tttatgagta ttaaagctgg atatagggtc
     2821 aatgattatg tgattaataa tgataaaata tttgtatttg gaatgagaaa tgtttatatt
     2881 attgttacac ttgtttgctt aataggtgca atattaattg gcttttattt atttaaatat
     2941 aataaaaatg aacaatagtt tttataatta tattcaaatt atgattatat tataagaaat
     3001 ttactaaaat acttcctttg agttaaattt aatttggaca tattgactta aaaatgtttt
     3061 attaatttga taaaatattt cttttcctat agtttataaa taaaaatcag ctgagcaaaa
     3121 tatttagtat attatgctca actgattcct ttattattta actaaaactt caatattttc
     3181 atcttttcca tctataacta tagtacatcc attatacaca tctccagcaa taagtttctt
     3241 tgcaattata gtttctaatg tatttcctat atatctctta agaggtcttg ctccatatac
     3301 agggtcatat ccttctctca ccataacatc ttttgctgaa tttgtaactt caatagtaat
     3361 atctttttct tttaatctat tttttaaatc tttcataaat atatctataa ttttcttgat
     3421 attttcttgg tctaaaggct taaacattat aatatcatct actctgttta aaaactcagg
     3481 tttaaatcta tgcttcattt cattcattac taaattgtta gtagtctctg ttatatttcc
     3541 tcctgcttct aatagataat tacttcctat attagaagtc attattataa gagtattttt
     3601 aaagtctact gtcttacctt tattatcagt aagtctacca tcatctaata tctgtaagaa
     3661 catgttaaat acatcctcat gtgctttttc tatctcatca aatagtatta cagaataagg
     3721 agcacgtcta actgcttctg taagttgacc accctcttca tatcccacgt accctggagg
     3781 aggtccaact agtcttgata cagcatgttt ttccatgtat tcagacatat ctattcttat
     3841 tatattatct tcactatcga ataaatttct agcaagagtt tttgcaagtt cagttttacc
     3901 aacacctgta gggcctaaga aaataaatga accaattggt tttctttcat cttttaatcc
     3961 tgctcttgca cgtattactg catttgaaac tgctgtaact gcttcatctt gtccaataac
     4021 tcttttatga agttcatctt ctagtttaag taatttttct ctttctcctt caactagttt
     4081 agttactggt atacctgtcc actttgatac aatgctagat atttcttctt cagtaacttc
     4141 ttcttttaaa agagaatttt cagaaccatc attcatattt tcttcgtact gctttatttg
     4201 ttcttctaac ttaggtattt caccatattt aacctctgct gctttgttga aatcatattc
     4261 tctttcatac ttttcagctt tacctttagc ttcgtctaat tgagccttta agtttttaat
     4321 atctaatatt tgactctttt ctttttcata ttttgctgtc atttcatcat ttttagattt
     4381 taactctgct atttcttttt gaatatcatc caatcttgat ttacttttat catcattttc
     4441 tttaagtaat gcttctcttt cagtttctaa tgtaaataac tttcttctaa caacatctaa
     4501 ttctgttggc aatgaatcta tttcactacg aatcatagct ccagcttcat caattaaatc
     4561 aattgcttta tctggtaaaa atctatcttg tatatatcta tgagaaagtt ttgctgctgc
     4621 tacaatagca ttatcatgta ttcttactcc atgatgtatc tcaaatcttt cttttaaacc
     4681 tctaagtata gatattgtat cttcaacagt aggttcttct gcaattacag gctgaaaacg
     4741 tctttcaaga gccttatctt tttctatata ctttctatat tcatcaaaag tagttgcacc
     4801 aatacagttc aattcacctc ttgcaagcat aggtttaata agattacctg catccattga
     4861 accttctgtt ttaccagctc caacaatagt atgaatctca tctataaaca gtataatttt
     4921 tccatcagaa ctttgaactt cttttaaaac tgcctttaat ctttcttcaa attcacctct
     4981 atattttgca ccagcaatta aagctcccat atcaagtgag aatacaatct tatcttttaa
     5041 accttctggg acgtctcctc ttactattct ctctgctaac ccttctacaa ttgcagtttt
     5101 acctacacct ggctcaccaa tcaatacagg attatttttt gttcttcttg ataaaattct
     5161 tataatcctt cttatttctt catctctccc aattacaggg tctaatttat ttttcttggc
     5221 taaatctact aagtttgttc catatctagc aagtgcatca tatgtgcctt ctgggtcttg
     5281 tgtttctact ctttgactac ctctcacttg tgataaaata tttaaaaagt cattcttatt
     5341 tatattatat tgttttaata ttttaccaac agctgatttt gattctgttt ccatcattgc
     5401 aagcataaca tgctctacac ttatatatga atctttaaat tctttagaaa tactctctgc
     5461 ctttataagc acctcattta tttttctagt agctgatact ccttgtgagt cagcaccttc
     5521 tccatatacc tttggaagct tatcaatttc aaagtttaca gtatttctca aagaatcaat
     5581 tgatatattc attttttcga ttatatttgg tatcaatcca tcctcttgat taataagtgc
     5641 tgagaaaaga tgaattacat ctacttgttg gttatgattt tttactgctt cgttataagc
     5701 ctcatttaaa cttttttgaa ctcttaaagt cattttttct acgtccatat aaatccctcc
     5761 attttaatat tatatccaaa taaattaaat tctctcattt ataattattg attttctaag
     5821 ctctaaaaga ctatcaagta aattctcgtt tttttgtata tcgaaactca atgttaaatt
     5881 aaatgttcca tctgaattaa aaaaatcgaa tacttctttt gattttgcta ttaaatacaa
     5941 gtcttcatct ataagtcttt tttcacctat taataaatac tgacatttat ctataaaact
     6001 ctttttaaaa tcaatagtgt atagattttc taaaatatta tattgataaa tagcaatatt
     6061 attaaacact tttaatatgt ttgaatttac aaaattgtaa atttgattat tgttatacag
     6121 tgatatttgt tcgtatatat attccctaaa taaaggatga atgttttgct taagttttaa
     6181 agtaacttca gctggtattt caaaataaag cttccagtac ttagagttat ttttttctaa
     6241 aataaaagct actttgttca aactggtaga actaatctcc tttagtgtac aattaaaatt
     6301 atagtgtctc aaactagttt tttcattctc aaatacatag tcaaaccact gtagaacatt
     6361 tctatcaagt tttaaactca tataaaaacc ttcttcctta tttactaacc ttattaggtc
     6421 tttttatctg attatattct gctaataaac tttctttatc agctctacta cctttatatg
     6481 ctatatgtgg atttatcata tactcaccat ttttcactct ttgtaaaaaa tccatttttt
     6541 tcagctccgt aaacaatgaa gataaagaag tttttggata ttcaaattca caagataatt
     6601 catataatgt ttttctaatc ttattatttt tatccataga gtttattaat tttaatggaa
     6661 ttaaatccct cttagtatac attccttcta ctactttaat ataattctta aaacaaacat
     6721 tcatatactt acttttattc ataattaaac ttttgctctc tgtatattcc tgatttaaat
     6781 ccttttttaa tgtcttatcc aaattaaata tctcttctca ctctccttat ctctaattga
     6841 taatataaat atattacaaa aaaatatgta tgtcaaaact gaaaatgcaa aaagttcacc
//
LOCUS       DBGJFF010000169         6886 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.173, whole genome shotgun sequence.
ACCESSION   DBGJFF010000169 DBGJFF010000000
VERSION     DBGJFF010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6886
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.173"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            109..2154
                     /locus_tag="ACOIAF_003492"
     CDS             109..2154
                     /locus_tag="ACOIAF_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HGZ2670472.1"
                     /translation="MKKHNILFVSTDDKINIDISKQLENIFGEFCNIDNLVYVNRINI
                     ELSSYELVVCSDNDIKEYIHNNIDKNIPIVIVHRTINIENINQIISIENDSDVMVIDA
                     YKESADETAKIIRKLGLIHINLIPYYPGCDKSKCEIGIITGSRNSIPQNIKQIIDIGD
                     KVIDINTVIEIFTKLNISIDKLHIIKEKYNEDTVSGYRYYTTMNKTMKSFLEIIDEGI
                     ASVDKLGKFIYCNKVFSNLVGIDQNEIISNNFMDLFSDKVIKKIFFQEDEVNDEVVNL
                     NNKKLIINKVNVYENNERIKSIISIKNISAIQVLEDKIQNKLQSKGFVSKYTFESVVG
                     ESKIIKEKINIARKIATTDFSVLILGENGTGKEIFAQAIHNESLRKNKPFVAVNLSSL
                     SDTLIESELFGYDEGSFTGAIKGGKMGIFERAHTGTIFLDEIGDISLDVQQRLLRVLQ
                     EKEVMRMGGSKIIPIDVRIIAATNKDLKKKIKEGSFREDLYYRINVLHIEIPRLRERK
                     EDIPLISKYFLDEINSNKCFTEESMQALTLYEWPGNVRELKNLIYYIDTIVEEDRVDY
                     EHLPEQFRFEKNNTLVNENFDSIILDFKQSNFFEESICILTSIETWNNKNILLGRNKL
                     QEILKEKDIVLSVDQIRKRIDKLKSHGLLLSGVKKQGSFITDEGKNFISYVKIKGVI"
     gene            2415..3614
                     /locus_tag="ACOIAF_003493"
     CDS             2415..3614
                     /locus_tag="ACOIAF_003493"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HGZ2670473.1"
                     /translation="MKSAYKGYEAYSYLEKGKDISSIEMCKGDKRVKEYLIELDDIQE
                     AKVKSIVDEKIFISLHEHPVLFPEHLERDIFEYNREGKQRCAYEALSRGYYDAIFDNL
                     MDGVCTITSNSGWKWDDILIDLGMRLCDLAHQDFVIKCEKVEDIYRAKSEGKVALIPT
                     LEGAAPIENELDRIDILYGFGIRLMGITYSESNALGSGLKEEKDGGLTNFGKKAVERM
                     NKIGMAIDCSHVGVQTTLDVIEYSKDPIILSHVGARSLWNTKRLTPDEVLIACAKKGG
                     VIGVEAAPHTTITEKNKEHSIESFMEHFEYIKNLVGIDHVAFGPDTVYGDHVKLHSVL
                     AAKFDIKKITGTKEHPKVEYVKGLENPTETSKNIVRYLVKHGYSDEEIEKVLGGNILR
                     VLKQVWK"
     gene            3666..4361
                     /locus_tag="ACOIAF_003494"
     CDS             3666..4361
                     /locus_tag="ACOIAF_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HGZ2670474.1"
                     /translation="MDYLKLANHPLLWIASTVAVSIVVVQSLIFAIKSCKVGKTMGIT
                     DKQIKSAVKSSAISAIGPSMTIFAGMVSLIVTMGGPIAWMRLSFIGSVIFESMSAGFG
                     TDALGITLGSPEMTKLAFTNAVWTMILGSLGWIIFTLLFGHKLDKITNVISSGKKSFI
                     PIISLGAMLGSFAYLNADRVLRFDNGTIACISGMAIMVILCMLEKKKNIKWLREWGLT
                     ISMFSGMIIASVI"
     gene            4378..5055
                     /locus_tag="ACOIAF_003495"
     CDS             4378..5055
                     /locus_tag="ACOIAF_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670475.1"
                     /translation="MSKVKSIYNEEYLPFMIRYGRLTLSLGIIAALVPGIILSFGFGI
                     MPPISALLASTMAIVSMSAPNYIIEPVSYSPILGIPGTYMSFLSGNISNMRLPCSIAA
                     QKAAEVESGTEEGSIISTIGIAVSILVNISILTIGVILGGSVLSKIPAEVVEKLNLIL
                     PALFGSVFGQVFLQDKKLGLVAIVISVLTIILSKQGIIPQSLVVLICVFGTILIARAM
                     YKDKLSD"
     gene            5166..6767
                     /locus_tag="ACOIAF_003496"
     CDS             5166..6767
                     /locus_tag="ACOIAF_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HGZ2670476.1"
                     /translation="MIYDLILKNGFIIDGTGNPGFYGDIAIKDNLIAKIDSKINSNIN
                     KEIDCCGKVMTPGFIDPHVHEEIVAILDGKFEKFLKQGVTTTINGNCGHSITPYSSEN
                     VYEYMYKNGLLLEEEKKYLIDKNKCWNNFTEYCDLISKSGISVNMGFLLGHGTIRWSV
                     MGGSKDRTPTEKEKNEITDIINDGMKSGAFGISTGLAYIPSKYADIDELVDIARQIKE
                     YDGIYTSHIRDYIGRYNAVKEAIEVGQKSGARVQVSHLSPVEIEAFDEILKARYNGVE
                     IMVDTVPRSSGHCMKKKRVIQFIMAISSSLFELGIDGVMDALRNEEGRTLILKEAFIL
                     GDRGSIILLNTKDINMEKKSIREIATQKGIDEDKLLLDLLLDGDEELIFCLGGMYRAD
                     FPDKLHDDKIIDNPFVMVGSDCLFSVGGDMSWFELQRRGAFPIFFNMYRKSGVRLEEI
                     VRRVTSLPARQFKIKNRGILKEGLIADIAVIDMDNYSYSRSEDIDFSKPQLLANGVEY
                     VIVNGKIALENGEITENKCGEVLKR"
BASE COUNT         2753 a          674 c         1305 g         2154 t
ORIGIN      
        1 aatagtaaat ggtacaaaat ttgattttta agatttaaaa cttttttaaa aagtgctaca
       61 atagaccaaa ttattacttt ctatactcgt taaacaggag gtgggactat gaaaaaacat
      121 aatattttat ttgtatcaac tgatgacaaa ataaatatag atatatcaaa acaattggaa
      181 aatatatttg gagaattttg taatatagat aacttggtat atgtaaatag gataaatatt
      241 gaactgtcaa gctatgagct tgtggtatgt tcagacaatg atataaagga atatatacat
      301 aataatattg ataaaaatat acctattgta atagtacatc gtactataaa tattgaaaat
      361 ataaatcaga taataagtat agaaaatgat tctgatgtta tggttataga tgcttataaa
      421 gagtcagcag atgaaactgc caagataatt agaaaactag gattaattca tattaatcta
      481 ataccatatt atccaggttg tgataagtct aaatgtgaga ttggaattat tacgggaagc
      541 agaaacagca taccacaaaa cataaagcaa ataattgaca taggtgataa ggtaattgat
      601 ataaatacag ttatagaaat atttacaaaa ctaaatatat ctatagataa attacatatt
      661 attaaggaaa aatacaatga agatacagta agtggatata ggtattatac aactatgaat
      721 aaaaccatga agagtttttt ggagattata gatgaaggta ttgcttctgt tgataaatta
      781 ggaaaattta tatattgcaa caaagtattt tctaatctag ttggaataga ccaaaatgaa
      841 attatatcta ataactttat ggacttattt agtgataagg ttatcaaaaa aatatttttt
      901 caagaagatg aagttaatga tgaagtagta aatttaaata acaaaaaact gataattaat
      961 aaagtaaatg tttatgaaaa taatgaacga ataaagagta ttataagcat taaaaatata
     1021 agtgcaattc aagtgcttga ggacaagatt caaaataaac ttcagtcaaa aggttttgta
     1081 tcaaagtata catttgagag tgttgtaggt gagagtaaaa taataaaaga aaaaattaat
     1141 attgcaagaa aaatagcaac tacagacttt tctgtgttga tacttggaga aaatggaaca
     1201 ggaaaagaaa tatttgccca ggcaatacat aatgaatctc taagaaaaaa caagccattt
     1261 gttgcagtta atctatcttc tttgtcagat acacttattg aaagtgaact ttttggctat
     1321 gatgaaggaa gttttacagg agcaataaaa ggtggaaaaa tgggtatatt tgaaagagca
     1381 catacaggaa caattttttt agatgagata ggtgatatat ctttagatgt acagcagaga
     1441 ttgctaagag tattacagga aaaagaagtt atgagaatgg gcggaagtaa aattatacca
     1501 atagatgtta gaattatagc tgcaacaaat aaagatttaa agaaaaaaat taaagaaggt
     1561 tcttttagag aagatttgta ttataggatt aatgtattac atatagagat acctagactt
     1621 agagaacgta aggaagatat acctcttata agtaagtatt ttttagatga aataaatagc
     1681 aacaaatgtt ttacagagga atcaatgcaa gctttaacat tgtatgaatg gccaggaaat
     1741 gtaagagagc ttaaaaattt aatttattat attgatacta tagttgagga agatagagta
     1801 gattatgagc atttgccaga acaatttaga tttgaaaaaa acaatacttt agttaatgaa
     1861 aatttcgatt caattatctt agatttcaaa caatctaatt tctttgagga aagtatatgt
     1921 atattgacca gtatagaaac ttggaataat aaaaatatat tattaggaag aaataaactg
     1981 caagagattt taaaagaaaa agatatagtt ttaagtgtag accaaattag aaaaagaata
     2041 gataagttaa agagccatgg tttactttta tctggagtaa aaaaacaagg tagttttata
     2101 actgatgaag gaaagaactt tatatcatat gtaaaaatta agggagtgat ataggggact
     2161 aaaaaggcaa aacaccctaa aaaaatccct ataatggata gaaataaata aaattatctt
     2221 taagattaag ataattcaaa aatattgaat aaataatcta agaagattat ttttaataaa
     2281 acagtagtaa atatcaacct tactactgtt tttatttttt gcaagattta ttatacaatt
     2341 aaaatttata ttttggcaca tcaattgcta cataggaaaa atataaaaaa ttgtagaact
     2401 tggaggatgt tgttatgaag tcagcttaca aaggatatga agcctatagt tatttagaaa
     2461 aaggaaaaga tatttcatct attgagatgt gtaaaggaga taaaagagtt aaagaatacc
     2521 ttattgaatt agatgatatt caagaagcta aggttaaaag tatagtggat gaaaaaatat
     2581 ttatatcatt acatgaacat ccagtattat ttccagaaca cctagaaaga gatatatttg
     2641 aatataatag ggaaggtaaa caaagatgtg cttatgaagc attatcaaga ggatattatg
     2701 atgctatatt tgataattta atggatggtg tatgtacgat aacatcaaat tcagggtgga
     2761 aatgggatga tatattaata gatttaggca tgagattatg tgatttagct catcaagatt
     2821 ttgtcataaa gtgtgaaaaa gtagaagata tatacagagc taaaagtgaa ggtaaagtag
     2881 cattgatacc aacattagaa ggtgcagcac caatagaaaa tgaacttgat agaatagata
     2941 tactgtatgg atttggtata aggttaatgg gtataacata cagcgaatcc aatgcattag
     3001 gttcaggact aaaagaagag aaagatggag gtcttacaaa ttttgggaaa aaagctgttg
     3061 aaagaatgaa taaaattgga atggcaatag attgctctca cgttggagtt caaactacac
     3121 ttgatgtaat agaatatagc aaggacccaa taatattaag ccatgttggg gcaagaagtt
     3181 tatggaacac aaagagacta actccagatg aagtattaat agcatgtgct aaaaaaggag
     3241 gggttatagg tgtagaagca gcacctcata ctacaataac agagaagaat aaagaacatt
     3301 caatagagtc atttatggaa cattttgagt atattaaaaa tttagtagga atagaccatg
     3361 tagcttttgg accagataca gtatatggcg accatgtaaa attacactca gtactagctg
     3421 ctaaatttga tataaaaaaa ataacaggaa caaaagaaca tcccaaagta gaatatgtaa
     3481 aaggtcttga gaacccaaca gaaacttcaa aaaacattgt tagatattta gtaaagcatg
     3541 gatatagtga tgaggaaata gagaaggtac taggtggaaa tatacttaga gtactaaaac
     3601 aggtttggaa atagatatat tagaaatatt aataaaattt gaggatttat agggaggttt
     3661 tataaatgga ttatttaaaa ttagctaatc atccactttt gtggatagcg agtacagtag
     3721 cagtatcaat agtagtggta caatccctta tatttgctat aaaatcttgc aaagttggta
     3781 aaacaatggg aataacagat aaacaaataa agtcagctgt taagagtagt gctatttcag
     3841 ctattgggcc ttcaatgact atatttgctg gaatggtatc gcttatagta actatgggtg
     3901 ggcctatagc atggatgaga ttatcattta taggttctgt aatttttgaa tcaatgtcag
     3961 ccggatttgg aacagatgca ttaggtataa ctttaggttc tcctgaaatg acaaaacttg
     4021 catttacaaa tgcagtttgg actatgatat taggttcttt aggatggata atattcacat
     4081 tactatttgg acataaacta gataagataa ccaatgttat atctagtggc aagaaatcat
     4141 ttataccaat aatatctcta ggagcaatgt tgggttcatt tgcatatcta aatgctgata
     4201 gggtgttgcg ttttgataat ggaacaattg catgtataag tggaatggct ataatggtaa
     4261 tattgtgtat gcttgaaaag aagaaaaata taaaatggtt aagagagtgg gggcttacaa
     4321 tatcaatgtt tagcggaatg ataatagctt cagtaatata aggggggagt tagttttatg
     4381 agtaaagtaa aatctatata caatgaagaa tatttacctt ttatgataag gtatggaaga
     4441 ttaacactat ctttgggtat tatagcagca ttagttcctg gaataatttt gagctttgga
     4501 tttggtatta tgcctccaat atcagcatta ttagcaagta caatggcaat tgtcagtatg
     4561 agtgctccaa attacataat agaaccagtt tcttattcac caatcttagg tataccagga
     4621 acttatatga gttttctttc tggtaatata tctaatatga ggctaccatg ttcaatagca
     4681 gctcaaaagg cagcagaagt tgaatcaggt acagaagaag gctcgataat atctacaata
     4741 ggaatagctg tatctatttt agtgaatata agtatactaa ctataggggt aattttagga
     4801 ggaagcgtac tatctaaaat tccagctgaa gtagtagaaa aattaaactt aattttacca
     4861 gctctatttg ggtcagtttt tggacaagta tttttacaag ataaaaaatt aggtctagta
     4921 gcaattgtaa tatctgtatt gactattata ttatcaaagc aaggtataat accacagtct
     4981 ctagtagttc ttatatgtgt atttggtact atattaattg caagagctat gtataaagat
     5041 aagcttagtg actaattaca atgaaataat agtgaatttt caaatatgaa atttaataat
     5101 ttgattttca aatataaaaa ttacattaag attatttaaa aattggtttt taaggaggga
     5161 atattttgat atatgattta atcttaaaaa atggatttat aatagatggt acaggtaatc
     5221 ctggttttta tggagatata gcaattaaag acaacttaat agcaaaaata gattctaaga
     5281 taaatagcaa tataaataaa gagattgatt gttgtggaaa agttatgaca cctggattta
     5341 ttgaccctca tgttcatgaa gaaatagtag caatactaga tggtaaattt gaaaagtttc
     5401 taaaacaagg agttacaaca actataaatg gtaattgtgg acattctata acaccatatt
     5461 cttctgaaaa tgtgtatgaa tatatgtata aaaatggtct attacttgaa gaagaaaaaa
     5521 agtatcttat agataaaaat aaatgttgga ataattttac agaatattgt gaccttatat
     5581 ctaaaagtgg tatttctgtc aatatgggat tcttgttggg gcatgggact ataagatgga
     5641 gtgttatggg aggttctaag gatagaactc ctacagaaaa ggaaaaaaat gaaattacag
     5701 atataataaa tgatggaatg aaaagtggtg cttttggaat atctactgga ttggcatata
     5761 taccaagtaa gtatgctgat attgatgaac ttgttgatat tgcaaggcag attaaagagt
     5821 atgatggtat atatacatct catattagag attacatagg aagatataat gcagtaaaag
     5881 aagccataga ggttgggcaa aaaagtggtg ctagggttca agtatctcac ttaagtccag
     5941 ttgaaataga agcatttgat gagatattaa aggctaggta taatggtgtt gaaataatgg
     6001 ttgacacagt accaagaagt tctgggcatt gtatgaagaa aaaaagagta attcaattta
     6061 ttatggcaat atcatctagt ctatttgaat taggtataga tggggtaatg gatgctctta
     6121 gaaatgaaga gggaagaaca cttatattaa aagaagcatt tatacttgga gataggggga
     6181 gtataatact tctaaacact aaagatatta atatggaaaa gaaatcaata cgtgaaatag
     6241 ctacacaaaa aggaatagat gaggacaagt tattactcga tttactttta gatggagacg
     6301 aagaacttat cttttgttta ggaggaatgt atagagctga tttcccagat aaactacatg
     6361 atgataaaat aattgataat ccatttgtta tggttggttc agattgtttg tttagtgtgg
     6421 gtggggatat gtcatggttt gaactacaaa gaagaggagc ttttccaatt ttcttcaata
     6481 tgtacagaaa gtctggtgtt agattggaag agattgtaag aagagttact tcattaccag
     6541 ctaggcaatt taaaataaaa aatagaggta ttcttaaaga agggttgata gctgatatag
     6601 cagttattga catggataac tatagttatt ctaggtcaga agatattgat ttttcaaagc
     6661 cacaattact agccaatggt gttgaatatg taattgtaaa tggaaaaatt gcattggaaa
     6721 atggagagat tactgagaat aaatgtggtg aagtattaaa aagataaggt tttaaaaata
     6781 ataggattta gaaagtaaat aaatatctaa gtaagtaaag acgaagtttg agatttattt
     6841 aaataaaaat catgcttcgt ttttatttat agtattaata tttatt
//
LOCUS       DBGJFF010000170         6762 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.174, whole genome shotgun sequence.
ACCESSION   DBGJFF010000170 DBGJFF010000000
VERSION     DBGJFF010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6762
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.174"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            18..497
                     /locus_tag="ACOIAF_003497"
     CDS             18..497
                     /locus_tag="ACOIAF_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HGZ2670477.1"
                     /translation="MSNKLIKNLLSGILQILFFLLGLVIVAGGFKSFMYLCFSGEATL
                     QGTIYGILMFILGVSYFIIIKSLIEVLGSSEYSLFVKENVKRFRIIGYLLLLNSLIEF
                     ISTFGTTGKGMRFLDLGFGFYFTVPVFVYFITSLMSFVIADGFVKAIKIKEDNDLTI"
     gene            1135..1512
                     /locus_tag="ACOIAF_003498"
     CDS             1135..1512
                     /locus_tag="ACOIAF_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HGZ2670478.1"
                     /translation="MMIRKIPQAELKVMKFIWKVDVTVTSKDVIEAMEQKYGWKQTTT
                     LTLLSRLVKRGFLDAQKIDRYTHYTIRVKHKDYLSFETKDFLSNIHDDSLRSLISALH
                     DDENIGKDKLDFLESYFDNLKDE"
     gene            1505..2653
                     /locus_tag="ACOIAF_003499"
     CDS             1505..2653
                     /locus_tag="ACOIAF_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HGZ2670479.1"
                     /translation="MNNYMLYVFENLIKTTIVCSLGICLLLFLKRYLFKKFSKRFNYY
                     IWLIIVFRMLLFLFNYTIVYEVKEPKENALGNNITQIDISTDNNLMLYVAYLWLFVTI
                     VIAVYTFIKYTRFKNLVVDVSYDIEDNDINCIYKNLLKELNIKKKIELRGSDELISPA
                     GMGLFKSYIFLPDYPYSKDELTWILKHELMHFKNKDILIKFLVLSVRIIYWFNPLVYV
                     MSNKVNLDCELCCDESVLVECSLKDKKEYALALIKSIKFSKNYNSKILTTEFDKTNLE
                     KRLESIVKKKGKSGILIAVLVFMTSITSFVEVDAQVRIDEGNNGMNNNINEFLLNKTT
                     DKIINIYYKDAPNEIRKFYEDKCELEGKIPRDYDIIEMDMKDYNQLIK"
     gene            2670..3272
                     /locus_tag="ACOIAF_003500"
     CDS             2670..3272
                     /locus_tag="ACOIAF_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HGZ2670480.1"
                     /translation="MLTKLRKIVAIMCCLLLTFTSVAFADTSKEKGGSKETVPEKNLD
                     SFTLSGYIYTYENAPQEIRDEHKQNCEEINIDPKPDDEIFVPESANLMNEDNSKGVYA
                     SYTVSYNIGAKTITIMSNEYLTISTTKVIRKGNSGKEVKAAQIMLTLLGYNVGIDSSF
                     GSKTYNAVVSFQKKYGLSADGIIGPATWDKLGRLTDPTLS"
     gene            complement(3365..4387)
                     /locus_tag="ACOIAF_003501"
     CDS             complement(3365..4387)
                     /locus_tag="ACOIAF_003501"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HGZ2670481.1"
                     /translation="MDYNDLLKSARENFNGTCKVCKICNGLACAGDVPGMGGKGSGSS
                     FIENRKSLEKIKINMRVIHNVSKPDTSIELFGRKMSSPIFAAPVSGTILNMGGKVSEK
                     EYIEPVVRGCSNSGIYAMVGDTNVDTFLLDNLDVLKDNRGNGIVFIKPWNNSKIIDKI
                     RLSEEAGAFAVGVDLDACGLINNQFQENPFSPKTIDEIRELVESTRLPFIIKGIMTVD
                     DALMAVESGASAIIVSNHGGRVLDYTPGTCEVLPDIAKSVKGKITILVDGGVRTGVDV
                     VKMLGLGADAVLMGRPFVTASFGGGLDGVEFFIEKVRNELCETMILTGCQNVKDIDGR
                     VIWNLL"
     gene            complement(4716..4898)
                     /locus_tag="ACOIAF_003502"
     CDS             complement(4716..4898)
                     /locus_tag="ACOIAF_003502"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670482.1"
                     /translation="MYKDKSDECIHLMTAYIDSISGYYSFIDTQLEDFMMKYGENIVD
                     SNLHSIMMLLCKWGLS"
     gene            5211..5630
                     /locus_tag="ACOIAF_003503"
     CDS             5211..5630
                     /locus_tag="ACOIAF_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3785 domain-containing protein"
                     /protein_id="HGZ2670483.1"
                     /translation="MSLYKFIYDDKEYLLKEENCSALINDEENPVQGVSISKIIDILN
                     EAEEVDFDVEYYQEACPLCLEGVKEKKKFFPFLEYHFYIFSKDGEYVISNISVDYKGL
                     SFNKLSRANKVDNSYIVSVIICENCQDYIVQIENCIV"
     gene            5761..>6762
                     /locus_tag="ACOIAF_003504"
     CDS             5761..>6762
                     /locus_tag="ACOIAF_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HGZ2670484.1"
                     /translation="MEEIDDTILDSMFKLTLNERLLTNNDFIENKIDVVKNLIDCYKT
                     EQNSLMLLKSNFLLALLYGIQGFSEKMKEYILISCKYIDKCLPKDYKFLARFYSQIAI
                     LSLKNEDVNKANLYMDKFNLICDENNFLIEEIIFKSQLIYIKASKCIESSVIIKEIET
                     LYIKIKEINDFNCKKIYFFIIGKIYFLFLDDALIAKVNFLKARKYADLLKDMQVSSLC
                     NIKIGECECSFENYECSMEYFNEVISNKKYRNVNIIQKYRASNNITKILIKTRNYPVA
                     INNLAKSEVYLEKIKNHSLKEVEKFDLFISLAMYYAKSNEKSFEQSICYLDRAKNILN
                     "
BASE COUNT         2690 a          738 c          962 g         2372 t
ORIGIN      
        1 ttaaaggagt tggcatgatg tctaataaac ttataaaaaa tttactaagt ggtatattac
       61 aaattttatt ttttctttta ggattagtaa ttgttgcagg aggatttaaa agttttatgt
      121 acctctgttt tagtggagaa gcaactctac aaggtactat ttatggaata ttaatgttta
      181 ttttaggtgt atcatatttt atcattataa aatcattgat agaagtatta ggtagttctg
      241 aatatagcct atttgtaaaa gagaatgtaa aaagatttag gattattgga tatcttttgc
      301 ttttaaactc tctaattgag tttatttcaa cttttggtac tactggaaaa ggaatgagat
      361 ttctagattt aggatttgga ttttatttta ctgttccagt atttgtatat tttataactt
      421 ctctgatgag ttttgtaatt gcagatggat ttgtaaaggc aataaagata aaagaggata
      481 atgatttaac tatttaggtg tgacacttta atatatagga gataaataac ccgaaaagag
      541 tgactaacta gtaatttaaa ataaaatata ttagggtata atacataaac tataatgcta
      601 aaattaaaag attataatac taaaatataa ttatgtttta attaaaataa agaactttat
      661 tagtagatat taaaagcaca tttttttata aaagtacttt tttataattt atgaaaggta
      721 taattataaa agtcttattc aataaaattt ataattaatt tagatatatt tgtaaataat
      781 cagtataaaa tagatgtttt aatatcataa aggcataatt agaaggttat taaaatttat
      841 tagatattac tctagtttat tttaaaaaaa agtattataa tattccttgt gtgaaaatat
      901 aggaattttt attaaatagt tttaatgtgt aaataacatt ctacactatt gtaattaaat
      961 atgtaaaaag taaaatattt aaatagatat attttaaaat ctaactttta atattgtaaa
     1021 aaaattagaa atttaacaaa aagtacaaaa attcacattt ttttcataaa gtgtttgact
     1081 aaaaaattca catgttgtag aattaaggta gcaggagttt tgagggaggt gtaaataatg
     1141 ataagaaaaa taccacaagc agaactaaaa gtaatgaaat tcatttggaa agtagatgtt
     1201 acagtgacat caaaagatgt tattgaagct atggaacaaa aatatggctg gaaacaaacc
     1261 accacactga cacttttatc taggctggta aaaagaggat tcttagatgc tcaaaaaata
     1321 gacagatata cacattacac aataagagta aaacacaaag attatttaag ttttgagaca
     1381 aaggatttcc taagtaatat acatgatgac tctttaagaa gcttaatttc agcattacat
     1441 gatgatgaaa atataggtaa agataagcta gattttctag aaagttattt tgataattta
     1501 aaggatgaat aattatatgc tatatgtttt tgaaaatctt ataaagacaa ctattgtatg
     1561 tagtcttggt atttgtctat tattattttt gaaaagatat ttatttaaga agtttagtaa
     1621 gagattcaat tattatattt ggttgataat agtctttaga atgttacttt ttctatttaa
     1681 ttatacaata gtgtatgaag taaaagaacc caaagaaaat gcattaggaa ataatattac
     1741 acaaattgat atttctacag ataataatct tatgctgtat gtagcttatt tatggttatt
     1801 tgtaactata gtcattgcag tttatacatt tataaagtat acaagattta aaaatctagt
     1861 tgttgatgta tcttatgata ttgaagataa tgatataaac tgtatctaca aaaacttgtt
     1921 aaaagaatta aatataaaaa agaaaattga attaagaggt tctgatgaat tgataagtcc
     1981 agcaggtatg ggattattta aatcttacat attcttacca gactatcctt atagtaaaga
     2041 tgaactaact tggattttga aacatgaatt aatgcatttt aaaaataaag atattttaat
     2101 aaagttttta gttttgtctg ttagaataat atattggttt aatccacttg tatatgtaat
     2161 gagcaataaa gtaaatctag attgtgaatt atgttgtgat gaaagtgttt tggtagaatg
     2221 ttctttaaaa gataaaaaag aatatgcact agcacttata aagtcaatta agtttagtaa
     2281 aaattataat agtaagattt tgacaaccga gtttgataag acaaatttgg aaaagagact
     2341 tgagagtatt gttaaaaaga aaggcaaaag tggaatacta attgctgttt tagtatttat
     2401 gacttctata acatcttttg ttgaagttga tgctcaagtt agaattgatg agggtaataa
     2461 tggaatgaat aacaatatta atgaattttt attgaataag acgactgata aaattataaa
     2521 tatttattat aaagatgcac caaatgaaat taggaaattt tatgaagata aatgcgaatt
     2581 agaaggtaaa atacctagag actatgacat aatagaaatg gatatgaaag attataatca
     2641 attaataaaa taattataag gaggatttta tgttaacaaa attaagaaaa atagtagcta
     2701 ttatgtgttg cttattgctt acttttacaa gtgtggcatt tgctgacaca agtaaagaga
     2761 aaggaggaag taaagagact gttccagaga aaaacctaga ttcttttact ctttctgggt
     2821 atatatatac atatgaaaat gcgccacaag aaataagaga tgaacataaa caaaattgtg
     2881 aagagataaa tatagaccct aagccagatg atgagatatt tgttccagaa tcagctaatc
     2941 taatgaatga ggacaatagt aaaggtgttt atgcaagtta tactgtttct tataatatag
     3001 gtgctaaaac tataacaatt atgagtaatg agtatttgac tataagtaca acaaaagtta
     3061 tacgaaaggg taatagcgga aaagaagtaa aagctgcaca aatcatgtta actttacttg
     3121 gttataatgt aggtatagat agttcttttg gaagtaagac atataatgca gttgtaagtt
     3181 tccaaaaaaa atacggtttg tctgcagatg gaataattgg tccagctact tgggataaat
     3241 tgggtagact aactgaccct actctttcat aggattaaaa tctaaaaata tattaaatag
     3301 tgtctcaata tgatttttca atttaaatga gacactattt aaatataaat gttataaaac
     3361 tattttacaa taaattccat ataactctac catctatatc cttaacattt tgacaaccag
     3421 tcaaaatcat agtctcacat aactcattcc taaccttctc tataaaaaac tccacaccat
     3481 caagaccacc gccaaaagaa gcagtaacaa aaggtcttcc catcaaaaca gcatcagcac
     3541 ccaatccaag catcttaact acatcaacac cagtcctaac tccaccatca acaagtattg
     3601 ttatctttcc ctttacagac ttagctatat ctggaagtac ctcacaagta ccaggagtat
     3661 aatcaagtac tcttccacca tgatttgaaa caataatggc actagcacca gactctacag
     3721 ccatcaaagc atcatctaca gtcataattc cttttataat aaaaggtaat ctagtggatt
     3781 caaccaattc tctaatctca tcaattgttt taggagaaaa aggattttct tgaaattgat
     3841 tattaataag tccacaagca tcaaggtcta caccaactgc aaaagcacct gcttcttcag
     3901 acaatctaat tttatctatt atctttgaat tattccaagg ctttataaat acaattccat
     3961 ttcccctatt atccttcaat acatctaaat tatccagtaa aaaagtatct acattagtat
     4021 cgccaaccat agcataaatt ccagaattag aacaacctct aacaacaggc tctatgtatt
     4081 ctttttcgga aactttacca cccatgttca atatagtacc tgatacaggt gcagcgaata
     4141 taggagaact catttttcta ccaaataatt ctatggatgt atctggcttt gaaacattat
     4201 gtatgactct catatttatc tttatttttt ctagactttt tctattttca atgaaagacg
     4261 aaccacttcc tttaccaccc ataccaggaa catctccagc acaagctaat ccattacaaa
     4321 ttttacatac tttacatgtt ccattaaagt tctctctagc tgattttagt aagtcattgt
     4381 aatccataca aaatctctcc tttttataat attaaaattg agtattacta tattgaaaat
     4441 atcttaattg taaatatagg gatgttatta tgatgagcta aactagataa tacatattag
     4501 atatttttta ttgttgtaga tagttaaatt atatcatatt agatagtcaa attttgaaaa
     4561 aaactaactt atttaagtat acattttgaa aaaattaaaa aagctatata tagattttta
     4621 aagaaatcta tatatagctt ttaagtttat gtcaatcttt tttgttaatg aataaataat
     4681 atagaataca atatacaaaa aagtttcttg aaatgctaag ataatcccca tttacaaagc
     4741 aacatcatta tactgtgtaa atttgaatca acaatgtttt caccatattt catcataaaa
     4801 tcctctaatt gtgtgtcaat aaatgaataa tatccactta tactatcgat gtatgcagtc
     4861 attaagtgta tacattcatc acttttatct ttatacatat atatctcccc ctatatacaa
     4921 tatataaatt tagataccaa atcgtatcat attaaatagt taagtgtgta acatctatgc
     4981 agttatatat ttatatcatt atattaataa ctaaagtgag ttttgacaat agattttggg
     5041 aaactggatg ttcaaagtgc aaacttggat tttaaaatca ttaattaaga tagcgcatag
     5101 gaatatatgt atacataact taattataaa gattataaac aattactttt attaacttta
     5161 tttaaatggt agaataactt ggagattaat aattataagg aggatataat atgagtttat
     5221 ataagtttat atatgatgat aaggaatatt tattaaaaga ggagaactgt agtgctttaa
     5281 taaatgatga agaaaatcca gttcaaggag taagtatttc aaaaataatt gatatactta
     5341 atgaagcaga agaagttgat tttgatgtag aatattatca agaagcatgt ccattatgtt
     5401 tagaaggagt aaaagaaaag aaaaaattct ttcctttttt agaatatcac ttttatattt
     5461 tcagtaaaga tggagaatat gtaataagca atatatctgt tgattataaa ggactatctt
     5521 ttaataaact atcaagggca aataaagttg ataatagtta tattgtaagt gtgataattt
     5581 gcgaaaattg tcaggattat atagttcaga tagaaaattg tatagtttag tgtaaagtat
     5641 gtcttaattt aagagaaatt tctaaattga gacatcaact tttttcaaaa atagtatgga
     5701 aaattactaa atttatgaga taatgtaagt cggaggtgag ttacaatata tagtaaatat
     5761 ttggaagaga tagatgatac aattttagat tcaatgttta aactaacctt gaatgaaaga
     5821 ttattgacta ataacgattt tattgaaaat aaaattgatg tagtaaagaa tttaattgat
     5881 tgctataaaa ctgaacaaaa tagtttaatg ttattaaaat caaatttttt gctagcttta
     5941 ttatatggta ttcaagggtt tagtgaaaaa atgaaagagt atattttgat ttcctgtaaa
     6001 tatattgata aatgcttacc caaagattat aaatttttag cacgatttta ttcacaaata
     6061 gctattttaa gtttaaaaaa tgaagatgta aataaggcaa atttatatat ggataaattt
     6121 aatcttattt gtgatgaaaa taattttcta attgaagaaa ttatttttaa atcacaactt
     6181 atttacatta aggcaagtaa gtgtatagaa tcttctgtaa taattaaaga gatagaaacg
     6241 ttgtatataa agataaaaga aattaacgat tttaattgta aaaaaatata tttttttatt
     6301 attggtaaaa tatatttttt gtttttggat gatgctctaa ttgctaaggt aaatttttta
     6361 aaagcaagaa aatatgcaga tttactaaag gatatgcaag tatcttcact atgtaatata
     6421 aagataggtg agtgtgaatg ttcgtttgaa aattatgaat gttccatgga atatttcaat
     6481 gaagtaataa gcaataaaaa gtatagaaat gtaaatataa ttcaaaaata tagagcatca
     6541 aacaacataa ctaaaatatt aataaagact agaaattatc ctgtcgcaat aaataattta
     6601 gcaaaatctg aagtatatct agaaaaaatt aaaaatcata gcttaaaaga agtagagaag
     6661 tttgatttat ttataagttt agccatgtat tatgcaaaaa gtaatgaaaa atcttttgaa
     6721 caatcaatat gctatttaga tagagctaaa aacatattaa at
//
LOCUS       DBGJFF010000171         6728 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.175, whole genome shotgun sequence.
ACCESSION   DBGJFF010000171 DBGJFF010000000
VERSION     DBGJFF010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6728
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.175"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(38..1192)
                     /locus_tag="ACOIAF_003505"
     CDS             complement(38..1192)
                     /locus_tag="ACOIAF_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese transporter"
                     /protein_id="HGZ2670485.1"
                     /translation="MEMLISASEEAFLHVGSMIGFFILLFGYINYKTSGNFTNIISKN
                     RKFQPLIGALIGAIPGCGGSLAIMPLYINGKLSFGAIIASLIASMGDAAFVLISSNIK
                     MYFFVTIVSTITGIITGQLVDYFKLEEKLGLKNRRKSDKYDISKNNPKENKEQHNHDE
                     IILDTLAKSHGNTNRLAFIITHGKGYKIYIGIIVIGFIFMSLAHSGLNLPIIEKLHSL
                     EEVIAVIGILFSIIYMWCFKKVFKNSNQKEEENKKISLREMLIHSVGEISFVITWIFI
                     AYLIYDLIILILGGDEYLVKLVLSTGVISVFIGAGLGLIPGCGIQIVLMSFYLKGTIP
                     LGAVIANSISQDGDALFPLLAMDKKSSLWAMIITTIPAILVGMIVYAFLG"
     gene            complement(1437..2276)
                     /locus_tag="ACOIAF_003506"
     CDS             complement(1437..2276)
                     /locus_tag="ACOIAF_003506"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HGZ2670486.1"
                     /translation="MNIKIAKNAGFCFGVKRAMKMAWDEVEKNDSGIYALGPLIHNKQ
                     AVAKYEEKGLKTVNEIDTIPNHENMIIRSHGVPENIYKEAKDKKLKIVDTTCPFVKKI
                     HTVVSEYHNKGYEIIVIGDMKHPEVIGINGWCENSAIIIKTLEQMENMEFDNSKRYCL
                     VAQTTINPELYISIVNKLSDKLEEIVFNDTICSATKTRQESAKELAKEVDCMIVIGGK
                     HSSNTQKLVKVCEDLVPTFAIETKDELDVNTLKKYKNLGITAGASTPNWIIEEVVTFL
                     ENL"
     gene            complement(2289..2885)
                     /locus_tag="ACOIAF_003507"
     CDS             complement(2289..2885)
                     /locus_tag="ACOIAF_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HGZ2670487.1"
                     /translation="MNFYRFVINIFKGFSKIFFKYEVIGAENIPDRGNIVIASNHKSN
                     LDPIFLAAAIENREIAAIAKKELFKVKPLGFILKKLHVMPINREKPDVSTIKTILRSV
                     RDGYVLGIFPEGTRIKGDSFGKAKAGLSVFTIKSKSKVVPVSIISKYKLFSKVIVYIG
                     EPISFEEHFKEKLSNDDHERISQEILEVIKQNYFKYSK"
     gene            complement(2936..3586)
                     /gene="cmk"
                     /locus_tag="ACOIAF_003508"
     CDS             complement(2936..3586)
                     /gene="cmk"
                     /locus_tag="ACOIAF_003508"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HGZ2670488.1"
                     /translation="MGNLVIAVDGPAGAGKSTIAKIVAKKLNINYIDTGAMYRAVTYK
                     CLKSGIDVNNEKEVIQIAENSDIDFKDNNIYLDKEVINEEIRTIEVSNNVSNVAKIKE
                     VRQLMVEVQRKIGMKNSVILDGRDIGSYVFPDADYKFFLVATPEERGNRRYKELCNKG
                     YNTTLEEVIEDIIRRDEIDSNREFAPLVKANDALEIDTTGKTIEEVVEEVVSKINL"
     gene            complement(3632..4885)
                     /locus_tag="ACOIAF_003509"
     CDS             complement(3632..4885)
                     /locus_tag="ACOIAF_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HGZ2670489.1"
                     /translation="MSKVIVVGGGASGMMAALSASKNNNEVILVERNGELGRKLRATG
                     GGRCNFTNNREIEDFFDKVVSNKKFLYSSFYTFTNKDLISYFESRNLEYKIEEENDHK
                     VYTKNDKSIEVIEVLNKDLLNHNVKIMYNKKVIDIITEEIALKDDSNKDKSKYLIKGI
                     ILDNGDKILGDKVIISTGGVSYSKTGSDGSMYKILKKHGHTLNKLYPALVPLTIEEKW
                     IKDLQGISMKNVEISCKIKKRKISKSGDMLFAHFGITGPCVLIMSSYINKIIEKEKVE
                     LNIDFLPNLSTDEISSIIRAFPNKNVLNNLKQILPQNFLKEIFSLLSLVDKKASDLSK
                     ADEIRIIEYIKNMKLTCNGTTGINTGMVTSGGISVKEINSSTMESKLVKNLFFTGEVI
                     DIDAETGGYNLQIAFSTGYLAGISV"
     gene            complement(4909..5550)
                     /locus_tag="ACOIAF_003510"
     CDS             complement(4909..5550)
                     /locus_tag="ACOIAF_003510"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HGZ2670490.1"
                     /translation="MGNTFYIVRHGQTDWNILGKTQGHGNSDLTPQGIEQAKELSEDI
                     GKYSIDYIFSSDLGRAMQTAQILGDKLNIEVQKTEALREMGFGVWEGLLIKEIQKDYS
                     DIYATWRNEPHLVNIPEGETLKIIKERVDAFIKELNEKYDNKNIILVTHSITLRVMLL
                     SFLESGMENIYRIKQDNTALNIVEFKDYGPVIVKMNDTSHIKNHVKINNSALE"
     gene            complement(5752..6165)
                     /locus_tag="ACOIAF_003511"
     CDS             complement(5752..6165)
                     /locus_tag="ACOIAF_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HGZ2670491.1"
                     /translation="MLELKNNLGISERKLKYPCIYKHFKGKYYATMGLSKAIDDIENI
                     CEIYGKENLIQNRNKYKLVIRHTEREEDIYVYRDLDGNFYHKKEEDTNDLVLYKTLYD
                     DTGIFARPLDMFLEKVDTDKYVNSIQEYRFEEVYK"
     gene            complement(6316..6657)
                     /locus_tag="ACOIAF_003512"
     CDS             complement(6316..6657)
                     /locus_tag="ACOIAF_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HGZ2670492.1"
                     /translation="MKEENFINVEYEDKRFSINSKEVLYIEKQEYEVKIYTESGNYEV
                     GENAPCIRKLLKEENFFKCSNSYIVNLDKCEFYLSNNCIFVNNNFIRILGSERKKKLK
                     KKLEKLYDYED"
BASE COUNT         2130 a         1183 c          630 g         2785 t
ORIGIN      
        1 tattattttt ttaatacttc tttattcata attatattta acctaaaaat gcataaacta
       61 tcatacctac aagtattgct ggtatagtag taataatcat agcccataaa gaagattttt
      121 tatccattgc taaaagtggg aaaagtgcat ctccatcttg agaaatagag ttggctatca
      181 ctgcaccaag tggtatagtt cccttcaaat aaaatgacat aagcacaatt tgtatcccac
      241 aacctggaat aagtccaaga cctgcaccta taaatacact tattacacct gttgataata
      301 ctaatttaac taagtattca tctccaccta aaattaatat tatcaaatcg taaataagat
      361 atgcaataaa aatccatgta attacaaatg atatttctcc aacagaatgg ataagcattt
      421 ctcgcaaaga aatcttttta ttttcttcct ctttttgatt agaattttta aatacctttt
      481 taaaacacca catataaatt attgaaaata atattccaat cactgctatt acttcttcca
      541 atgaatgtaa cttctcaatt ataggaagat taagtccaga gtgagcaagt gacataaata
      601 taaaaccgat tactataatt cctatataaa ttttatatcc ctttccatgt gttattataa
      661 atgcaagtct atttgtattt ccatgagatt tagctagagt gtctaaaatt atttcatcat
      721 gattatgttg ttctttattt tctttagggt tatttttact tatatcatat ttatctgatt
      781 ttctacggtt ttttaaccct aatttttctt ctagtttaaa gtaatccact aattgtccag
      841 ttattattcc tgtaattgta ctaactatag tgacaaaaaa atacatttta atatttgatg
      901 aaataagtac aaatgctgca tctcccatac tagctattaa acttgcaatt attgctccaa
      961 agcttaattt tccatttata tacaatggca ttattgccaa acttccgcca caacctggta
     1021 tagctccaat taaagcacct attaagggtt gaaattttct gttttttgat atgatattag
     1081 taaaatttcc actagtttta taattaatat atccaaataa caaaataaaa aatccaatca
     1141 tagaacctac atgtaaaaac gcctcttcag aagcggatat caacatttcc attaatttac
     1201 ctcctagact aattttgcaa caattatatt atattctatt ttctattgat aatcaatatc
     1261 aatatttctt ttgaatttct aatattaaaa agtgcgcatc ttatactctt agtttttcat
     1321 cttaagatac acactttttg atattaggtt atctatttac cacatgtaat atgaaatcaa
     1381 aggtggatat tatgataaat tataacaatg tccacctatt tttattacct atcaaattat
     1441 aaattttcaa ggaatgttac aacttcttct attatccaat taggtgtaga tgcccctgct
     1501 gtaataccca aatttttata ttttttaagc gtatttacat ccaattcatc tttagtctct
     1561 attgcaaaag ttggtactaa gtcttcacaa acctttacta acttttgagt atttgaactg
     1621 tgtttacctc ctataactat catacaatca acttctttag ctaactcttt agctgattct
     1681 tgtcttgttt ttgtagctga acaaattgta tcattaaata ctatttcttc taatttatct
     1741 gatagtttat taactatact tatatacaac tctggattta ttgtagtctg tgcaacaaga
     1801 caatatcttt tggagttatc aaattccata ttttccattt gttctaaggt ctttattatt
     1861 atagctgaat tttcacacca tccatttata cctataactt ctggatgctt catatctcca
     1921 ataactatta tttcatagcc tttattatga tattcactta ctactgtatg tattttcttt
     1981 acaaatggac atgtagtatc cacaattttt aattttttat ctttggcttc cttatatata
     2041 ttttcaggaa ctccatgaga ccttattatc atattttcat gatttggtat tgtatcaatt
     2101 tcattcacag tcttaagacc tttttcttca tattttgcta ctgcttgctt gttatgaata
     2161 agaggaccta aagcatatat gccagaatca tttttttcaa cttcatccca tgccattttc
     2221 attgctcttt ttactccaaa acaaaagcca gcattttttg ctatttttat gttcataact
     2281 ttaccccctt attttgaata tttaaagtaa ttttgtttta tcacttctaa aatctcttgt
     2341 gatatccttt catggtcatc attactcaat ttttccttaa aatgttcttc aaatgatatt
     2401 ggttctccaa tatatacaat tactttacta aaaagtttat atttagatat tattgatact
     2461 ggaacaactt tagatttact ttttattgta aatacagaaa gacctgcctt agccttacca
     2521 aaagaatctc cttttattct agtaccctct ggaaatattc ctaatacata accgtctcta
     2581 actgacctta aaatagtttt tattgtagat acatctggtt tttctctatt tataggcata
     2641 acatgcaatt tttttaatat aaatcccaat ggttttactt taaacaattc tttctttgca
     2701 attgcagcta tttctctatt ttctatggca gcagccaaga atatagggtc aagattcgat
     2761 ttatgatttg atgctattac tatatttcct ctatctggaa tattctctgc acctataact
     2821 tcatatttaa aaaatatttt agaaaaaccc ttaaaaatat ttattacaaa tctataaaaa
     2881 ttcacgttta tctcccttca acactacatt tattttcatt atgatgcata aaaaactaaa
     2941 gatttatttt agatacaact tcttctacaa cttcttctat agtttttcct gtagtatcta
     3001 tttctagtgc atcattagct tttaccaatg gtgcaaattc tctatttgaa tcaatttcat
     3061 ctcgtcttat tatatcctca attacttctt ctaaagtagt attatatcct ttattacaaa
     3121 gttctttata gcgtctattt cctctttctt ctggagtagc tactaaaaag aatttgtaat
     3181 ctgcatctgg gaaaacatat gaacctatat ctctaccatc tagtattact gaatttttca
     3241 ttcctatttt tcgttgaact tcaaccatca actgtcttac ttcttttatt tttgctacat
     3301 tagaaacatt attgcttact tctattgttc ttatttcttc atttataact tctttgtcca
     3361 aatatatatt gttatcttta aaatctatat ccgaattttc agctatttga ataacttctt
     3421 tttcattatt gacatcaata ccacttttta aacacttata agtaactgct ctgtacatag
     3481 cacctgtatc tatataattt atatttagct tttttgctac tatcttggct attgtacttt
     3541 ttccagctcc tgctggacca tctacagcta taactaaatt acccatatat acacctctat
     3601 ttattcatat ttaattatta aaaaattata tttatacact tatacccgca agatatccag
     3661 ttgaaaatgc tatttgtaaa ttatatccac cagtttctgc atctatatca attacttcac
     3721 ctgtaaaaaa taagttctta actaatttag attccatagt agaagaatta atttctttaa
     3781 ctgaaatacc accagaagta accataccag tgtttatacc tgtagtccca ttacaagtta
     3841 gcttcatatt ttttatatac tcaataattc taatttcatc tgccttagac aaatcacttg
     3901 cctttttatc aactaaagat aatagagaaa aaatctcttt taagaaattt tgaggtagta
     3961 tttgcttaag attatttaga acatttttat ttggaaatgc tctgattata ctagagattt
     4021 catcagtact taaatttggt aagaaatcta tatttaattc taccttttcc ttttcaataa
     4081 tcttgttaat ataagaagac attataagaa cacaaggtcc tgttatccca aaatgggcaa
     4141 atagcatgtc tccagatttt gatattttcc ttttcttaat cttacaagat atttccacat
     4201 tcttcatgga tattccttgt aaatctttaa tccatttttc ctctattgta agtggaacta
     4261 aagcaggata tagtttgttt aatgtatgtc catgcttttt aagaatctta tacattgaac
     4321 catctgaacc cgtctttgaa taactcactc ctccagttga tataataacc ttgtctccta
     4381 gtattttatc tccattatct aaaataatcc cttttattag atactttgac ttgtccttat
     4441 tagaatcatc ttttagagca atttcttcag ttataatatc tataactttc ttattataca
     4501 ttatctttac attgtgattg agtaaatctt tatttaatac ttctattact tctatagatt
     4561 tatcattttt agtgtatact ttgtggtcat tttcttcttc aatcttatat tccaaatttc
     4621 tactttcaaa atacgaaata aggtctttat ttgtaaatgt ataaaaacta ctatagagaa
     4681 attttttatt actgacaact ttatcaaaaa aatcttctat ctctctatta ttggtaaaat
     4741 tgcatctacc gccaccagtt gccctaagtt ttcttcctaa ttctccattt ctctcaacta
     4801 atattacttc attattgttt tttgaagctg acaaagctgc catcattcca gaagctcctc
     4861 caccaactac tataacttta ctcataaatt tctccttata attgattcct actccagtgc
     4921 agaattattt atctttacat gattttttat atgacttgta tcattcattt ttactataac
     4981 aggcccataa tctttaaatt ctactatatt taaagcagta ttatcttgct taattctata
     5041 tatattttcc atacctgatt ctaaaaatga caacaacata accctcaatg taattgaatg
     5101 tgtaacaaga attatatttt tattatcata tttttcattt agttccttta taaaagcatc
     5161 tactctttcc tttataatct taagagtttc gccttctggt atatttacta agtgtggttc
     5221 atttctccat gttgcatata tatctgaata atctttttga atttctttta ttaaaaggcc
     5281 ttcccaaact ccgaatccca tttctctaag tgcctcagtt ttttgaactt caatattgag
     5341 cttatctcct aaaatttgag ctgtttgcat tgctctccca aggtcgctac taaaaatata
     5401 atcaattgaa tacttgccaa tatcttcaga aagttctttt gcctgttcta taccttgagg
     5461 cgtaaggtca gaattaccat gaccttgtgt ttttcccaat atattccaat ccgtttgtcc
     5521 atgtcttact atataaaaag tatttcccat atgtcctcct aatatatgcc tattttttat
     5581 taaatataaa ttatcacttc tcaaaactta attatatcat ataatacttt actttgtttc
     5641 tattttataa tctaatctaa catattattt acaataaatc tcatttgtat ttctgcaaat
     5701 tattgtactg ttaaagaatt tttctaaatt tataaacata tttctcttat attatttata
     5761 tacttcttca aatctgtatt cttgaattga attgacatac ttatctgtat ctactttttc
     5821 taaaaacata tccaaaggtc ttgcaaatat tcctgtatca tcatataagg tcttgtataa
     5881 aactaaatca ttagtatctt cttctttttt atgataaaag tttccatcta aatccctata
     5941 aacatagata tcttcttctc tttcagtatg tctaataact aatttatatt tatttctgtt
     6001 ttggattaaa ttttctttac catatatttc acatatgttt tctatatcat caatagcctt
     6061 gctaagaccc attgtagcat aatatttccc tttgaaatgt ttatagatac aaggatattt
     6121 taactttctt tcactgatac ctaagttatt ctttaattca agcattttat tataatctcc
     6181 catatctcgt ccctttcttt taaaaagtat tttattctga aataagttac ttgttttata
     6241 ttttatcata atttaaaatt tattaataat acaatcaata aataaagagg ctgtataaaa
     6301 tacagcctct caatattaat cttcataatc atataatttt tctaatttct tttttaattt
     6361 cttttttctt tctgaaccta aaattcttat aaaattatta ttcacaaaaa tacaattatt
     6421 gctgagataa aattcacact tgtctagatt tacaatataa ctattactgc atttgaaaaa
     6481 attttcttct ttcaatagtt ttcttataca aggagcattt tctccaactt cataattacc
     6541 actttcagta taaatcttta cttcatactc ttgcttttct atgtacaaaa cttctttact
     6601 attaattgaa aaacgtttat cctcgtattc aacattaata aaattttcct ctttcatttc
     6661 catccctcca agtactttta ttaatttaat tttagtgttt tttatcaata taatctatcg
     6721 gaaatata
//
LOCUS       DBGJFF010000172         6684 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.176, whole genome shotgun sequence.
ACCESSION   DBGJFF010000172 DBGJFF010000000
VERSION     DBGJFF010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6684
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.176"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            304..1218
                     /locus_tag="ACOIAF_003513"
     CDS             304..1218
                     /locus_tag="ACOIAF_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HGZ2670493.1"
                     /translation="MELYEAIFYRKSVRNFSSKKIKKPLMEEIKRSCKNLEYLNEDLN
                     IKAHVVDRGHIIHFLMGKECKVKAPHYIVVTSDKGEDYLQNIGFATEGVVLRLTTLGI
                     ATCWLEGNLKREDILEFVDIGNKNFEEDEEDDLDYLAGIKTEEDDENKIENPYIIIAF
                     GYPAKNEELFRTRYARPDRKPVKDMINKINRNRKWKDILELTRRAPSVKNSQPWYFHK
                     DERGLHLFEKRPKKHCEDMNKVSLGVALRHFDIACIKNKIDVSYEKLPIRNKIGKSYF
                     ITVVEHVKPEEETQEENVTLEKEESQDE"
     gene            1242..1877
                     /locus_tag="ACOIAF_003514"
     CDS             1242..1877
                     /locus_tag="ACOIAF_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1847 domain-containing protein"
                     /protein_id="HGZ2670494.1"
                     /translation="MYKCGKCNLRGCSNGNMDKLMKICPTREIELQQKAKQLYEEEEN
                     RKIAYNAGLVEAEGYCQYTRLKEIIEFAKKCGYKKIGIAFCIGLKREMQTVQKIFEHH
                     GFEVFSVICKNGAMPKSTIGLEASQTISKCSDEVMCNPIGQALFLNEAKVDLNVILGL
                     CVGHDTLAMKYMEAPITTLVVKDRVTGHNPVAAIYNAESYFEKKLFDKDEE"
     gene            2253..2969
                     /gene="pyrF"
                     /locus_tag="ACOIAF_003515"
     CDS             2253..2969
                     /gene="pyrF"
                     /locus_tag="ACOIAF_003515"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HGZ2670495.1"
                     /translation="MINGKEKLIVAIDTDEFDKAKELIDRLEDSVDIFKVGLEQYVAT
                     KGKTIDYLKEKGKKIFLDLKFHDIPNTMKSAVRAAVRDNVWLMTIHVSDMEGMRQCAL
                     IAREEAERLNIEKPLVVGVTVLTSLSNQDLQDIGCNMTTEELAIKRAKLAKESGIDGV
                     VCSAQEVDKIVEACGTDFITVCPGIRPKSAEVGDQKRVVTPSDAIKKGAHYLVVGRPI
                     TKAENPRESAINIVREIENA"
     gene            2995..4038
                     /gene="carA"
                     /locus_tag="ACOIAF_003516"
     CDS             2995..4038
                     /gene="carA"
                     /locus_tag="ACOIAF_003516"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HGZ2670496.1"
                     /translation="MKGKLILEDGTVFEGKAFGYLKEAIGEVVFNTSMAGYGELLTDA
                     TYYGQIVTMTYPLIGNYGINLEDVESNKVQVKGLIVREKSDNPNNFRCEMDIDTYLKQ
                     NKVIGLEGIDTRALTKIIRNNGTKRGIITLENVSLQDVQSKLEEHCNKDSVKNVTRKE
                     IENIKGTGAKVVVIDFGVKNSVLESLKACNCDITIVPAMTTVEEILNINPDLILLSNG
                     PGNPEDINDIVENIKKLIGKKPLTGIGLGHQILALALGGKTSKLVFGHRGGNHPVKCL
                     ETGKVFITSQNHGYNVSEMPENTEVTHINLNDNTIEGMRHKELPIYSVQYIPEYTTKE
                     DLDCIFNKFLALI"
     gene            4091..>6684
                     /gene="carB"
                     /locus_tag="ACOIAF_003517"
     CDS             4091..>6684
                     /gene="carB"
                     /locus_tag="ACOIAF_003517"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HGZ2670497.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQSLKEEGIEV
                     VLINSNPATIMTDKEVADKIYIEPLTIEFIEKIIEKERPDSLLAGMGGQTGLNLAVEL
                     YEKGILDKYGVKIIGTSVESIKKGEDRDIFREVMKEINQPVIVSDIVTDLQAGLDYAL
                     TIGYPVVVRPAYTLGGTGGGIADNEEELREILSHGLQLSPVGQVLIEKSIKGWKEIEY
                     EVIRDSKGNCIVVCNMENIDPVGVHTGDSIVVAPTQTLSNKECAMLKKASLDILNAVE
                     VQGGCNVQFALNPHSFEYAVIEINPRVSRSSALASKATGYPIAKVSSKIALGYTLDEI
                     ENAVTKKTYACFEPTIDYVVAKIPKWPFDKFKKANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHTPSEERSIEELKKRVVVPDDERLFDLAEMIRRGYKVEMIEQ
                     ITGIDKWFINKFKWIVEQEEKLKVMKIEDLTKDYLLELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIKPAYKMVDTCAGEIDAISPYYYSTYEQYDEVVVSDKKKVIVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMDIETIIINNNPETVSTDFDTSDKLYFEPLTEEEVLSI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFDDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWSLNEGIEVANELGYPVLVRPSYVLGGQGMEITYNEEKLSQYLQDAFDRD
                     HKNPVLIDKYLTGREIEVDAICDKEDILIPGIMEHLERAGVHSGDSTTMYPSQNISDE
                     IKEKIVEYTKKIALDLNVLGMVNIQFIEFQNELYIIEVNPRASRTVPYISKVSGVPIV
                     DLATKCMLG"
BASE COUNT         2778 a          692 c         1316 g         1898 t
ORIGIN      
        1 aataatgaga tagatattta taaatgaaaa aaaatttttc tggcaaagct taattaaaaa
       61 ggtaatagct agtgttgtta tttaaattag catagaaaat caaataaaaa aattatgcta
      121 atgtatagac attgataagg tagaatttaa gccttttgaa tgctatatta aactagagta
      181 gatattctta tgaatttcaa aatagggcag gaggaaaaag gaaataaaac tataaaaaat
      241 ataaagtatt tatataaatt tatagagttt atacgtcaga agatttcagt taggagggca
      301 taaatggagc tttatgaagc cattttttat agaaaatctg taagaaattt ttccagtaag
      361 aagataaaaa agccattgat ggaagaaata aaaagaagtt gtaaaaattt agaatactta
      421 aatgaagatt taaatattaa agcacatgtt gttgatagag gtcatataat acattttttg
      481 atgggtaagg aatgtaaagt aaaggcacct cattatatag tagttacatc agacaaaggt
      541 gaggactatt tacagaatat aggatttgct acagaaggtg tagttttgcg ccttacaaca
      601 ttgggaatag ctacttgctg gttggaagga aacttaaaga gagaagatat cttggaattt
      661 gtagatattg ggaataagaa ttttgaagaa gatgaggaag atgatttaga ttatctagct
      721 ggaataaaaa cagaagaaga tgatgaaaac aaaatagaaa atccttatat aataatagca
      781 tttggatatc cagcaaaaaa tgaagagttg tttaggacga gatatgctag acctgataga
      841 aagccagtaa aagatatgat aaataagata aataggaata gaaagtggaa agatatctta
      901 gaattgacaa gaagagctcc atctgtaaaa aattctcaac cgtggtattt ccataaggat
      961 gaacgaggtc ttcatttatt tgaaaagaga cctaagaaac actgtgaaga tatgaataag
     1021 gttagcttag gtgtagcctt aagacatttt gatatagctt gtataaaaaa taaaatagat
     1081 gtttcatatg aaaaacttcc tataaggaat aaaataggaa aatcatattt tataactgta
     1141 gtcgagcatg tgaaaccaga agaagaaacc caagaagaaa atgtaactct agaaaaagaa
     1201 gaatcgcaag atgaataaaa ttttggaagg ggatattgga tatgtataag tgtggaaaat
     1261 gtaatcttag aggctgttct aatggaaata tggataaatt aatgaagata tgtccaacta
     1321 gagaaattga actacaacaa aaagcaaagc aactttatga agaagaagag aatagaaaaa
     1381 tagcttataa tgctggatta gttgaagcag aggggtattg tcaatatact agactaaaag
     1441 agattataga gtttgctaaa aagtgtggtt ataaaaagat tggcatagca ttttgtattg
     1501 gactaaaaag agaaatgcaa acagtgcaaa aaatatttga acatcatggg tttgaagtat
     1561 tttctgttat atgtaaaaat ggggcaatgc ctaagagtac aattggacta gaagcatctc
     1621 aaacaatatc aaagtgttca gatgaggtaa tgtgcaatcc aataggacaa gctttatttt
     1681 taaatgaagc taaggttgac cttaatgtaa ttcttggatt atgtgttggt cacgatactt
     1741 tagccatgaa atatatggaa gctcctataa caactttagt tgtcaaagat agagtaactg
     1801 ggcataatcc agttgctgca atatataatg cagaatcata ttttgagaaa aaattgtttg
     1861 ataaagatga agaataattc aaagacttat tgacataaaa agcaattagt ggtttaatta
     1921 taaaaaagat aaaactttaa attatcctat agatacataa agttatataa ataaagaaaa
     1981 atattacagc taaattcctt aaagtagtcc agagagacta aagggttata gataatgtta
     2041 gtatataacc tctagtaaat cttctagagg tttttgatga gtataaaata aaaatttaaa
     2101 tataataaag tcttttaaac tagtccagag agactgggaa agatatctca tagctaatga
     2161 atagagttat gtatagataa aatccttatg cctcttttgg tgtgaggatt tttttatgat
     2221 tatttaggct ttattaaaaa ggagatgtaa aaatgataaa tggtaaagaa aaattgatag
     2281 ttgcaattga tactgacgaa tttgataaag ctaaagaact aatagacaga ttagaagata
     2341 gtgtagacat atttaaggta gggctagagc aatacgttgc cacaaaaggt aaaacaatag
     2401 attatcttaa agaaaaaggg aaaaaaatat ttttagacct taaattccat gatataccaa
     2461 atactatgaa aagtgcagtg agagctgcag tgagagataa tgtttggtta atgacaatac
     2521 atgtatcaga tatggaagga atgagacaat gtgcactaat tgctagggaa gaagcagaaa
     2581 gattaaatat agaaaagcca ttagtagttg gagtaacagt tttaacttca ttaagcaacc
     2641 aagatttaca ggacatagga tgtaatatga caacagaaga gttagctata aagagagcta
     2701 aactggcaaa agaatcagga atagatgggg ttgtttgttc agcgcaagaa gtagataaaa
     2761 tagtagaagc atgtggaact gattttataa cagtttgccc aggaataaga cctaaatcag
     2821 cagaggttgg agaccaaaaa agagttgtaa ctccatcaga tgcaataaaa aaaggtgctc
     2881 attatttagt tgttggaaga cctataacaa aagcagaaaa tccaagagaa tcagctatta
     2941 atattgtaag agaaatagaa aatgcttaga taaaatattt gggaggtagc ttacatgaag
     3001 gggaaattaa ttttagaaga tggaacagta tttgaaggaa aagcatttgg atatctaaag
     3061 gaagctattg gagaagttgt atttaatact tcaatggctg ggtatggaga actattgaca
     3121 gatgcaactt attatggaca aatagttact atgacttatc cacttatagg aaactatgga
     3181 ataaatttag aagatgttga atcaaataag gtgcaagtaa aaggacttat tgtaagagaa
     3241 aaaagtgata acccaaataa ttttagatgt gaaatggata tagacacata tctaaagcaa
     3301 aataaggtaa taggacttga aggaatagat acaagagcat taactaaaat tattagaaac
     3361 aatggaacta agagaggaat tataacttta gaaaatgtat cattacaaga tgttcagtct
     3421 aaattagaag agcattgtaa caaagattca gtaaaaaatg ttacaagaaa agaaattgaa
     3481 aatataaaag gtactggagc taaagttgta gttatagact ttggtgttaa aaatagtgta
     3541 ctagagtctc ttaaggcttg taactgtgat ataactatag taccagcaat gactacagta
     3601 gaagaaattt taaatataaa tcctgactta atcttattat ctaatggccc aggaaatcca
     3661 gaggatataa atgatatagt tgaaaatata aaaaaattaa taggcaaaaa gccattgaca
     3721 ggaataggtt taggacatca aattttagca ttggcattag gaggaaagac ttcaaagctt
     3781 gtatttggac atagaggagg taatcaccca gttaaatgtt tagaaactgg aaaggtattt
     3841 ataacttctc aaaatcatgg atataatgtt tcagaaatgc cagaaaatac agaagtaact
     3901 cacataaatt taaatgacaa tactatagaa ggaatgagac ataaggaatt accaatatat
     3961 agtgttcagt acattccaga atacacaaca aaagaagatt tagattgtat atttaataaa
     4021 ttcttagcat taatatagat ttagtattat ttacagatag taaaaaccat ttagataagg
     4081 agaaaaattt atgcctaaat tagatagtat aaagaaaact ttagtattag ggtcaggacc
     4141 aataataata ggtcaagctg cagagtttga ctattcagga acacaagctt gccagtcttt
     4201 gaaagaggaa ggaattgaag ttgtattaat aaatagtaat ccagccacta taatgactga
     4261 taaagaagtg gcagataaaa tatatataga accattaaca atagaattta tagaaaaaat
     4321 aatagaaaaa gaaagaccag acagtttact tgcaggtatg ggtggacaaa cagggctaaa
     4381 tctagcagtt gaactttatg aaaaaggcat actagataaa tatggagtaa agataatagg
     4441 tacttcagta gaatctatca aaaaaggtga agatagagat atatttagag aagtaatgaa
     4501 agaaataaat cagcctgtaa tagttagtga tatagttaca gatttacaag caggtcttga
     4561 ttatgcactt acaattggtt atccagtagt tgtaagacca gcatacacac taggtggaac
     4621 tggtgggggt atagctgata atgaggaaga attaagagaa atactttcac atggtctaca
     4681 attaagtcct gttggacaag tattaattga aaagagtata aaaggttgga aagaaataga
     4741 atatgaggta ataagagatt caaagggaaa ttgtatagtt gtatgtaaca tggaaaatat
     4801 agacccagtc ggagttcata caggagacag tatagttgtt gctccaactc aaactttaag
     4861 taacaaagaa tgtgcaatgc ttaaaaaagc atctttagat atattaaatg cagtagaagt
     4921 tcaaggtggt tgtaacgttc aatttgcact taatccacat agttttgaat atgcagttat
     4981 agagataaat ccaagagtta gtagatcatc agctttagca tcaaaagcta caggatatcc
     5041 aatagctaaa gtttcatcaa aaatagcttt aggttatact ttagatgaga tagaaaatgc
     5101 agtaactaaa aaaacttatg cttgttttga accaactata gattatgtgg tggcaaaaat
     5161 accaaaatgg ccttttgata aatttaaaaa agcaaataga aaattaggaa ctaagatgat
     5221 ggcaactggt gaaattatga gtataggaag taattttgag gctgctattc ttaaagggat
     5281 aagatcactt gaaacaggaa aatattcatt agttcacaca ccatctgagg agagaagcat
     5341 tgaggaatta aagaaaagag ttgtagtacc tgatgatgag agactatttg atttagctga
     5401 aatgataaga agaggctata aagtagaaat gatagaacaa attacaggta tagataaatg
     5461 gtttataaat aaatttaaat ggatagtaga gcaagaagaa aaattaaaag ttatgaaaat
     5521 tgaagattta actaaagact atcttcttga attaaagaaa aagggattct ctgataaagg
     5581 tatatctgac ttaatgaaga taagtccaga aaaattatat gaattgagaa gtctatacaa
     5641 tatcaaacca gcatataaaa tggttgatac ttgtgcaggt gagatagatg ctatatctcc
     5701 ttactattat tcaacttatg agcaatatga tgaggtagtt gttagtgata agaaaaaggt
     5761 gatagtatta ggttctggtc caataagaat aggtcaaggt atagaatttg actattgttc
     5821 agttcactgt gtaaaatctt taagaaaaat ggacatagaa actataatca taaacaataa
     5881 ccctgaaact gtaagtacag actttgatac atcagataag ctgtattttg aaccattaac
     5941 agaagaagaa gttttaagta taatagaaaa agaaaagcca gaaggtgtaa tactacagtt
     6001 tggtggacaa acagctataa aattggctaa atttttacat gaaaaaaata tacctatatt
     6061 gggaactgat tttgatgata tagatgcagc agaagataga gaaaaatttg atgatttact
     6121 agagagacta gatataaata gaccaaaagg aaagggtgta tggtcactta acgaaggtat
     6181 tgaagttgct aatgagttag gttatccagt attagttaga ccatcttatg tacttggtgg
     6241 tcaaggtatg gaaataactt ataacgaaga aaaattatct caatacctac aagatgcatt
     6301 tgatagagac cataaaaatc cagtacttat agataaatat ctaactggta gagaaataga
     6361 agtagatgct atatgtgata aggaagatat attgatacct ggtataatgg aacatttaga
     6421 aagagctgga gttcactctg gagatagtac aacaatgtat ccaagtcaaa atatatctga
     6481 tgaaataaaa gaaaagatag tagaatacac taaaaaaata gctttagacc ttaatgtgct
     6541 tggtatggtt aatatacagt ttatagaatt tcaaaatgaa ttatatataa ttgaggttaa
     6601 ccctagagct agtagaacag ttccgtatat aagtaaagta agtggagtac caatagttga
     6661 cttggctact aagtgtatgt tagg
//
LOCUS       DBGJFF010000173         6674 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.177, whole genome shotgun sequence.
ACCESSION   DBGJFF010000173 DBGJFF010000000
VERSION     DBGJFF010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6674
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.177"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            <1..1097
                     /locus_tag="ACOIAF_003518"
     CDS             <1..1097
                     /locus_tag="ACOIAF_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGZ2670498.1"
                     /translation="YLSACNVSTNMIGIIIGAYGISQCLLRLPVGVFADSINKHKIFI
                     LSGIFFAGLASLIRVFSPGGNGFLIANILSGISSSTWISYMVHYTNFFSTEHQQKATS
                     TIILVNNLGMLIGFVMSTLFYEKLGMTALCIMSVLSSVIGTILALNIKNHNSKKIPEK
                     ISIKKYLSICKDKRLVFFSFMALIQQGIQMSTTMSFTTQILKNLGSSSTLIGLSSIFY
                     MVCAVASSGFASTKFCTKYGPKFWIPLVFILVSAYCFLVPTIKNINIIFVLQVLPGIS
                     TGILLSYLTSESMKNVPKHQKSTAMGFFQAIYAIGMSVFPMIVGKISEYTSIQYGYFI
                     LAIIAILASILSYTYYKKVDLTLNYDIIQS"
     gene            1123..1815
                     /locus_tag="ACOIAF_003519"
     CDS             1123..1815
                     /locus_tag="ACOIAF_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HGZ2670499.1"
                     /translation="MKSLNATEKIIDYIKLNILNGTFKINSKLPSERKIADLFNISRI
                     PVRNAINILCKEGILRAVPYSSPIVEGFKKVDLFDDGEIYKNHNIQEFYVESLRARQL
                     IESEATKLAILNATSKEFQKIKYTYLKSIEELDKVSHGLIEECYDADLQFHKEIILAS
                     HNPIFIKYYELIPKTVSSNQYFGFKYRNSLKDMISHHNKIMIAFDSKDSYLGYTSMYN
                     HLEDVIQLFQHD"
     gene            complement(2032..2334)
                     /locus_tag="ACOIAF_003520"
     CDS             complement(2032..2334)
                     /locus_tag="ACOIAF_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIBC"
                     /protein_id="HGZ2670500.1"
                     /translation="MNKKIVAVCESERSLNSLKTAANDLGFDLVYEIQENDNIKNELS
                     IYDIEDASIVLFVTSDSIESIEKIDRFIDREFYEVDPKYIIEDAKSIVSEILIDLN"
     gene            complement(2356..2766)
                     /locus_tag="ACOIAF_003521"
     CDS             complement(2356..2766)
                     /locus_tag="ACOIAF_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HGZ2670501.1"
                     /translation="MNKKKILVPIDGTERSMHSLEFIKGIFKKDEVEVEIMNVKELVF
                     IDGISLAEEIKNSENLGRRILDKAAEIMGEYDVKVHFTFGYPGDEIIRKAKEDNVDFI
                     VMTKSTKKGLTRMIGSVTASVVKQAKCIVMIVPE"
     gene            complement(2943..4871)
                     /locus_tag="ACOIAF_003522"
     CDS             complement(2943..4871)
                     /locus_tag="ACOIAF_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HGZ2670502.1"
                     /translation="MVWHIEYEIFSAIIVIFLMVYFFRSKFIPTLQNKIYCALLIFSF
                     LFIISNILGSFCLNNIDKIPIFITFLLNQIYLLLLPIPAALVSFYVMAIIYQDIRYMK
                     KRLLFLSIPLIICIILSITNPFTHILFGLSKETGYIRGCGYVATFASFFFYAIYSAFL
                     SFKYRKAMHESKIWAIRGFLIVSIVAIILQAIFLQYLLTGTACACSLLLTYLSMQNRG
                     LIIDDLTGVLNRQSFIQELDLNINTEQSGFIITIALDDFKFMNETFGTKNGDIALKEV
                     GKYLIQIHDVNHVYRIGGDIFSIVVNSQLGIEPDDIIRKIEERFKKPWLVEEISFNLA
                     TSIAVVYYPENAETTEDVIVAIDFSIHEAKRSESRRVVYADASISEKIKKKHVIKNCL
                     KEALKNDGFEVYYQPIFSNSRGRFTSAEALLRLEHKELGFIPPDEFIPIAEQTGLINL
                     IGLVVFEKVCRFIASDEFENLKLDNIAVNLSVVQCMQKNLADDLLYLMDKYNVSPTKF
                     KLEITETVAMGSFNIIKETMERLIDLGVKFALDDFGIAYSGVTNMLSLPFSVIKLDKS
                     LIWSMNEDSRHKLTVETIIALINKLNMKAVAEGVETIKCAEDLIAMECEYLQGYYFSK
                     PVPEDVFKNLLKENTFTS"
     gene            complement(5047..5535)
                     /locus_tag="ACOIAF_003523"
     CDS             complement(5047..5535)
                     /locus_tag="ACOIAF_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HGZ2670503.1"
                     /translation="MKITVLYQTRSGNTERVAKLIEDGAKKVDGVDVKLMRLDNIDLD
                     YLNESKAVIFGTPTYLANTTWEVKKWFDEDSKKVNLAGKLGAVFATCDYICGGPDVAI
                     LTIVGHLMVKGMLVYSGGGSLGKPFIHLGHVHSIEGPELQDEKAIIFGERIATKAKEL
                     FA"
     gene            complement(5671..>6674)
                     /locus_tag="ACOIAF_003524"
     CDS             complement(5671..>6674)
                     /locus_tag="ACOIAF_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HGZ2670504.1"
                     /translation="DKNDIFESIDTEDFDNKTEEECLELVRKNGLNLIYIKNQTEEIC
                     LEAVRQNCNAIKCVQNQTEKICIEAVKQDWRMLEFVEEPTEEICMLAINQDGTALKYI
                     ENQTEELCLRAVEKNGAALEYVKKQTEEICIEAVRNSEFGLARVKNQTEKICMEAVKH
                     CSYNLKWVENQTEEICMEAVRQNGLDLKFVKNQTETICLRAVRQNGMALEFVKEQTVG
                     ICLKAVRQYGMALKFVKEQTEEICTEAIKQDKRALSFVKGDKEKYKALYDNNEPFAKR
                     YVRNVIEKENRALIKKGEENAKIKIAGKLYDRGMSFEDISDIVEIEISKLKVSLGVF"
BASE COUNT         2238 a         1154 c          716 g         2566 t
ORIGIN      
        1 catatttaag tgcatgtaat gtatctacaa atatgattgg tataataata ggtgcatatg
       61 gaatatcaca atgtttatta aggcttcctg ttggagtatt tgctgattct ataaataaac
      121 ataaaatatt tatactgtct ggaattttct tcgctggttt agcatctctt ataagagtct
      181 tttctcctgg aggcaatggt tttcttatag ctaacatttt atctggaata tcatcatcaa
      241 cttggatttc ttacatggtt cactatacca actttttttc tacagaacat caacaaaaag
      301 ctacaagtac gataatattg gtaaacaatc taggaatgtt gattggattt gttatgagta
      361 ctttatttta tgaaaaatta ggaatgacag ccttatgtat catgagtgta ttgtcttcag
      421 ttattggaac gattctagcc ttaaatataa aaaatcataa ttcaaaaaaa atacctgaaa
      481 aaatatcaat taaaaaatat ttatctatct gcaaggataa aagacttgta tttttttcct
      541 ttatggcatt aattcaacag ggtattcaaa tgtctacaac aatgtcattt actactcaaa
      601 ttcttaaaaa cttaggttct agttctaccc tcataggtct atcttctata ttttacatgg
      661 tgtgtgctgt tgcttcttct ggctttgctt ctactaaatt ttgtactaag tatgggccaa
      721 aattttggat tcctttagtt tttattttag tttcagccta ttgcttttta gttccaacaa
      781 taaaaaatat taatataata tttgtactac aagtactacc aggtatatct acaggtatac
      841 tcctttctta ccttactagc gagtctatga aaaatgtacc aaaacatcag aaatctactg
      901 caatgggttt ttttcaagca atttatgcta taggaatgtc tgtatttcca atgatagttg
      961 gtaaaatatc ggaatatact tcaatacaat atggatactt tatcttggct attatagcta
     1021 ttttggcctc tatcctgtct tatacatatt ataaaaaagt agatttaact cttaactatg
     1081 atataataca atcataaaaa aattatatgt ttaggagttt ttatgaaaag cttaaatgcc
     1141 actgaaaaaa ttattgatta tattaaatta aacattctaa atggaacttt taaaattaat
     1201 agtaaattac caagtgagag aaagattgct gacctattta acataagtag aatacctgta
     1261 agaaatgcca taaatatact ctgtaaggaa ggaatactta gagctgtgcc ttattcaagt
     1321 cctatagttg aaggatttaa aaaagttgac ctatttgatg atggtgaaat ctataaaaat
     1381 cataacatac aagaatttta tgtagaatct ttaagagcaa gacaacttat cgaaagcgaa
     1441 gctactaaac tagcaatttt aaatgctaca tctaaagaat ttcaaaaaat aaaatatact
     1501 tacctaaagt caatagaaga attggataag gtttctcatg gtttgataga agaatgttat
     1561 gatgctgact tgcaatttca caaagagata attttagctt ctcataatcc aatttttata
     1621 aaatattatg agttaatacc taaaacagtt tcttctaacc aatactttgg ttttaaatat
     1681 agaaattctc tcaaggatat gattagccac cataacaaga taatgattgc atttgatagt
     1741 aaagactctt acttaggtta tacatcaatg tacaatcatt tagaagatgt aattcagtta
     1801 tttcaacatg attaaactta tattttacct taaatgcaac taatatataa aaatggagtg
     1861 tctcaaagtg aactttttag ttatgaggta ctcttttttg tatgaaatca aatatatttt
     1921 catcatttca acaatgaaaa agagtcttat ctatattttg atacaacctc tttatttctt
     1981 gtgtaaaata tataaaaagg cgtcgctcta ttcttagtgc gacacccacg attaatttaa
     2041 gtctattaaa atttcactta ctatagattt tgcatcctct attatatact ttggatcaac
     2101 ttcataaaat tctctatcta taaatctatc aatcttctct atgctttcta tagaatcact
     2161 tgtaacaaat aacactatgg atgcatcttc tatatcataa atagaaagtt cattttttat
     2221 attatcattt tcttgaattt cataaactaa atcaaatcct aaatcatttg cagctgtctt
     2281 aagagaattt aaacttcttt cactttcaca aactgctact atctttttat tcatatttca
     2341 aatacctttc taattctact ccggtacaat catcactata cattttgctt gtttaactac
     2401 acttgctgtt actgaaccta tcattcttgt aagacctttt ttagtagatt ttgtcataac
     2461 tataaaatct acattatctt cttttgcttt tctaattatc tcatctcctg gatatccaaa
     2521 agtaaaatgt actttaacat catactctcc cattatctct gctgctttat ccaagattct
     2581 tcttcctaaa ttttcagaat tttttatctc ttcagccagg gatatgccat caataaatac
     2641 tagttctttt acattcatta tttctacttc aacttcatct tttttaaata ttccttttat
     2701 aaattccaaa gaatgcatac ttctttctgt cccatcaatt ggcactaata ttttcttttt
     2761 attcatgtca catcttcctt tctcacttta ggataattaa ataaaatata taataaatct
     2821 ttactccatt aattaatatt aataatatgt ataataaatt taatatttat tattgtgtta
     2881 attttataat atatcgctgg cagtgtcaat gaacaaagtt gtaattaaaa aaataaaggg
     2941 tgttaacttg taaaagtatt ttctttcaac aaatttttaa aaacatcttc tggaactggt
     3001 ttagaaaaat aatacccttg caaatattca cactccatag ctattaaatc ttcagcacac
     3061 ttaatggttt caacaccttc tgctacagcc ttcatattca atttatttat aagtgctata
     3121 atcgtctcaa ctgtaagttt atgcctacta tcttcattca tagaccaaat caaactctta
     3181 tctaacttaa ttactgaaaa tggtaaagaa agcatattag taactccaga ataagcaatt
     3241 ccaaaatcat caagtgcaaa cttaacacct aaatcaatta atctttccat tgtttcttta
     3301 attatattaa aagagcccat agccacagtc tcagtaattt caagtttaaa tttagttggt
     3361 gatacattat atttatccat aagataaagt aaatcatcag ctaagttttt ttgcatacat
     3421 tgtactacag aaagattcac agctatatta tccaatttta aattttcaaa ctcatcacta
     3481 gctataaatc tacacacctt ctcaaagact actaatccaa ttaagttaat caatcctgtt
     3541 tgttctgcaa ttggaataaa ttcatcagga ggtataaaac ctaactcttt atgttctagc
     3601 ctaagtaatg cctctgctga agtaaacctg cccctgctat tagaaaatat aggttggtag
     3661 tacacttcaa acccatcatt ttttaatgct tcttttaaac aatttttaat aacatgtttc
     3721 ttctttatct tttcacttat agacgcatct gcataaacaa ctcgccttga ctcagaacgt
     3781 tttgcttcat gtatagagaa atctatagca acaattacat cttctgtcgt ttctgcattt
     3841 tctgggtaat aaacaacagc aatactagtt gctaaattaa aacttatctc ttctactagc
     3901 caaggctttt taaatctttc ttctatcttt ctgataatat catctggttc tatacctaat
     3961 tgactattga caacaataga aaatatatct ccccctattc tatatacatg gtttacatca
     4021 tgtatctgaa tcaaatattt tccaacttct tttagtgcaa tatctccatt ttttgttcca
     4081 aaagtttcat tcataaactt aaaatcatca agcgcaattg taataataaa tcctgattgt
     4141 tcagtattga tattcaaatc tagctcttga ataaaagatt gtctattgag tacaccagta
     4201 aggtcatcaa taataagacc tcgattctgc atagaaagat atgtcaaaag taaactacat
     4261 gcacatgcag ttcctgtaag aagatactgt aaaaaaattg cctgtaagat aattgccaca
     4321 attgaaacta taagaaaacc tctaatagcc catatttttg attcatgcat tgcttttcta
     4381 tatttaaaag ataaaaatgc tgaatatatt gcataaaaga aaaaagatgc aaaagttgca
     4441 acatatccac aacctcgtat ataacctgtt tcttttgaca atccaaatag aatatgcgta
     4501 aaaggatttg taatagataa gattatacaa attataagtg gtatagataa aaataataat
     4561 ctttttttca tataccttat atcttgataa ataatagcca taacataaaa tgatacaagt
     4621 gctgctggta ttggcaataa caataagtat atctgattca ataaaaaagt aataaaaatt
     4681 ggaattttgt ctatattatt tagacaaaat gaacctaaaa tatttgaaat tataaataaa
     4741 aatgagaata ttaaaagtgc acaatatatt ttgttttgta aagtaggtat aaatttactt
     4801 ctaaaaaagt ataccattaa aaaaataact ataattgcag aaaaaatctc atattctata
     4861 tgccatacca taaacttccc tcgctattga cgtttgattt taaatatttt aataatataa
     4921 tttatttata attatattat aattcatctt gagtaatttt atggaaaatt ttttatttta
     4981 taacttttac ttcaaatctc cattaaaacc ctaaatttac tctaatcatt actatgcata
     5041 tagaaattaa gcaaatagtt cttttgcctt agtagcaatt ctttcaccaa atattatagc
     5101 cttctcatct tgtaactctg gaccttcaat tgaatgtaca tgtcctaaat gtataaatgg
     5161 ctttcctaaa gaaccacctc cagaatacac aagcattcct ttaaccatca aatgtcctac
     5221 tatagtaagt atcgcaacat ctggacctcc acatatataa tcacatgtag caaaaacagc
     5281 tcctaatttt ccagctaaat taactttttt tgaatcttca tcaaaccatt tttttacttc
     5341 ccaagttgta tttgctaaat aagtaggtgt tccaaatatt actgctttac tttcatttaa
     5401 ataatctaaa tcaatattat caagtctcat aagctttaca tctactccat ccaccttttt
     5461 agctccatct tctatcagct tagcgaccct ttctgtgttt ccacttcttg tttggtataa
     5521 tactgtaatc ttcaacttta attccccctt atctaataaa aataatttat tatacatcaa
     5581 attatatcac taattttgct gtcctacaaa gagctagtcg attatttttt tattaactat
     5641 ttcccataaa aaacacctac atttattact ttaaaaaaca cctaaactca cttttaactt
     5701 gcttatttca atctcaacta tatctgatat atcttcaaaa gacatgcctc tatcataaag
     5761 ttttcctgca attttgattt ttgcattttc ttctcctttt ttaatcaatg ctctgttttc
     5821 tttttctatt acatttctaa catatctctt tgcaaatggt tcattattat catatagagc
     5881 cttgtatttt tccttatctc cttttacaaa actaagtgct cttttatctt gttttatagc
     5941 ttctgtacaa atttcttctg tttgttcttt tacaaatttt aatgccattc catattgcct
     6001 tactgccttt aagcatatac caacagtctg ttcctttaca aattctaatg ccattccatt
     6061 ttgtctcact gctcttaaac agattgtttc tgtttgattt tttacaaatt tcaaatcaag
     6121 cccattttgt ctcactgctt ccatacatat ttcttctgtt tgattttcta cccattttag
     6181 attatagcta cagtgcttaa ctgcttccat acatattttt tctgtttgat ttttcactct
     6241 tgccaatcca aattcgctgt ttctaactgc ttctatacat atttcttctg tttgtttttt
     6301 tacatactcc aaagccgcac catttttttc aacggctctt aagcataact cctctgtctg
     6361 attttctata tattttaatg ctgttccatc ttggttaata gccaacatgc aaatttcttc
     6421 tgttggctct tccacaaatt ctaacattct ccaatcttgt ttaactgctt ctatacatat
     6481 tttttctgtc tgattctgaa cacattttat tgcattacaa ttttgtctaa cagcctctag
     6541 acatatctct tctgtttgat tttttatgta tattaaattc aatccatttt ttcttactag
     6601 ctctaaacat tcttcttctg ttttattatc aaaatcctca gtatctattg attcaaatat
     6661 atcattttta tctt
//
LOCUS       DBGJFF010000174         6583 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.178, whole genome shotgun sequence.
ACCESSION   DBGJFF010000174 DBGJFF010000000
VERSION     DBGJFF010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6583
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.178"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(619..1179)
                     /locus_tag="ACOIAF_003525"
     CDS             complement(619..1179)
                     /locus_tag="ACOIAF_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HGZ2670505.1"
                     /translation="MLKKLKKMLLITCCLFMMSSTAFAETQNSEGKSFTISGYTFMYE
                     NAPQEIKDKYKENCLDIGVEPQPNDEIFITPSELKTYSNIDMYSARSSSFTVTYHYSY
                     YKVTGDKNYTVNTSTIVKYGNSGNPVHLVQLLLKKVGYALEVDSQFGNTTLSKVKSFQ
                     GSYGLTVDGIVGSATWKKFASATNLD"
     gene            complement(1225..2208)
                     /locus_tag="ACOIAF_003526"
     CDS             complement(1225..2208)
                     /locus_tag="ACOIAF_003526"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HGZ2670506.1"
                     /translation="MSNLILTDILRTSITSSLVICFLLLLRTTIFRIFNKGFNYYIWL
                     IVIVRLLFPFSYFTYTISDSKFQYKPILNEVDYSNTVLIIYLTIVILLAIYRFIKYLK
                     FKSLVIDLSYDIENLEINNLYNNLLKELNIEKHIKLKYTYEINTPSFFGLFDNYILLP
                     PHNYNLNELHWILKHELIHYKSKDLYIRYLVLFLKCVYFFNPFIYLLDRIIYHSCELH
                     CDEVVLKGCSLENIKSYANTILDSIERSSRDSNNYTTGLHRKSNFEKRVYDMFKNKGK
                     SGILIALILCLLSSFTYFKFDSIISKPINHSLDAPLPVNTGKEKSFTITID"
     gene            complement(2221..2583)
                     /locus_tag="ACOIAF_003527"
     CDS             complement(2221..2583)
                     /locus_tag="ACOIAF_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HGZ2670507.1"
                     /translation="MIEKIPRAELKVMMLFWDTDKVLTSRDIVNIMSEEWKQTTTLTL
                     LSRLSKRGFLEPTKKPTHTTYKILIDKKSYLEFATSDFVKNVHNDSIVSLVDSVNKEK
                     IGKDELASLRAFIKKLKK"
     gene            3825..4472
                     /locus_tag="ACOIAF_003528"
     CDS             3825..4472
                     /locus_tag="ACOIAF_003528"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670508.1"
                     /translation="MNKKLEKPFVYKREYDLTGYDVEILQKYELEQAIYVYVGSSCAY
                     NMRARSSKWRYHIRTNNKSICCNIKNFIHNLELFYKMELKLSDNIINDKLYYSNIAEF
                     EEFETLEKAREVESTIISQYQFLDSINHMLKQKIILLSNKDSVLNITKNGNTNYLKVK
                     NKYIEKHKNKPIMRYHINCQFNTDGSVKSITQEFEPILELNKKNTLSRPSRVFLK"
     gene            4747..6384
                     /gene="repA"
                     /locus_tag="ACOIAF_003529"
     CDS             4747..6384
                     /gene="repA"
                     /locus_tag="ACOIAF_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011161239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HGZ2670509.1"
                     /translation="MEQLDSKYKLKKFLMAVFRDGIGQGNNLIDNEYVRVFQNNKSNS
                     KQLELGEEFKEYSKTTFFKNIDDIVEFTFAKNIYYENTFFNLCTTDGKAGTNENLINR
                     YALGFDFDKKELGQGFNYKDIINLFTKIGLHYHILVDSGNGFHVYVLINKTNNIKLVS
                     EVTNTLINKLGADKQANLSTQVLRVPYTYNIKNTTKQVKIIHQDKNIYRYDIEKLAKK
                     YCKDVKTVGNTNTKYILDSKLPNCIVDILKNGSKDGHKNLDLQKIVVTLRLRNKSLSQ
                     VISVAREWNYISQNSLSNSELEYQVKYMYEKLKTVNFGCTGCEFNSDCWNKIESDFIY
                     SDEDTLFNMPHKHSKDLKYKNRKGVKIMTGNQLFIYNVLLNNKDRELNIDDIMELITY
                     KRKKKVKNIVMSEKTLRETLKELQHNDYITKTKGVTKLGIKDTYNVKEVRCNIDKQYT
                     ISYFVTMAVIWGIISTEELRLYTHMRYKQDLLVKDDKIKGNILRINQEELAKDLGVTQ
                     QRISNMIESLLDTKILDVWETKINDRGFMYYTYRLNK"
BASE COUNT         2682 a          692 c          917 g         2292 t
ORIGIN      
        1 ctaattagta ggtgcttttt taaaacaagt taaaaatcaa aaatagtatt agtaagcatt
       61 ggaaatgcta gattctaaaa tagaaaagta aaaaattggt gcactatcta aacttatcta
      121 tatcgctttt tccgtcgttt ggttctctag ttacgataca ggggatatgc ttatattgag
      181 ttatagtact aatcagtgct taatatagtt aataaaatta tagttaccat agtttagtaa
      241 ctatgatgta tgttagttag aaacttgcat ttcatgagtt gtaatatctt aaagtcagta
      301 gttttgattt taaaatatta attgcactgt tacaaatgac tttattaata aaaaattagg
      361 cagcttaaaa aaagtttaga ataaatttgc taggctttgc taagctattc ttttaaaaat
      421 aactttacaa aacaaacaaa taaggactta taaatataga taaaaaaaga aaattgaaaa
      481 ggattataga gacttacaag gctttaaata tatattctta attgtaaaat tatgtaatta
      541 aaatatttac tttattataa aaagacatga taaacttatt atcatgtctt ttgttaaact
      601 tatttatatg taaaaattct aatcaaggtt tgtagcagat gcaaattttt tccatgttgc
      661 actaccaaca attccatcta ccgttaagcc ataactgcct tgaaaacttt taactttgct
      721 aagtgtagtg ttcccaaatt gactgtctac ttctaaagca tatccaactt tttttaatag
      781 caattgtact aaatgaacag gatttcctga atttccgtat tttacaatcg tagaagtgtt
      841 gacagtatag tttttatctc cagtaacttt gtagtatgaa taatgataag ttactgtaaa
      901 tgaacttgac ctagctgaat acatgtctat gttgctataa gtttttaatt cactaggggt
      961 aataaaaatt tcatcattag gttgtggctc tacacctata tccaaacaat tttccttata
     1021 tttatctttt atttcttgtg gtgcattttc atacataaat gtgtaacccg aaatagtaaa
     1081 gctttttcct tcactatttt gtgtttctgc aaaagctgtg ctactcatca taaataaaca
     1141 acaagtaatt agcaacattt tttttaattt ttttaacata attcctccct ataattttga
     1201 atatatttga ataccagcaa atatttaatc tattgttata gtaaaagatt tttctttgcc
     1261 tgtattaact ggcaatggtg catctaagga gtgattaatt ggctttgata taattgaatc
     1321 aaatttaaaa tatgtaaatg atgacaataa gcataaaatc aatgctatta aaataccact
     1381 cttaccctta tttttaaaca tgtcataaac tcttttttca aaatttgatt ttctgtgtaa
     1441 tccagtagtg tagttatttg aatctcttga acttctttca attgaatcta aaattgtgtt
     1501 agcataactt tttatatttt caagggaaca accttttaat acaacttcat cacaatgtaa
     1561 ttcacagcta tgatatataa ttctatcaag tagataaata aaagggttaa agaaataaac
     1621 acactttaaa aataaaacca aatatctaat gtataaatct ttacttttgt aatgaattaa
     1681 ttcatgtttt agaatccaat gcagttcatt taaattataa ttgtgtggtg gcaaaagtat
     1741 ataattatca aataatccaa aaaatgaggg cgtattgatt tcataagtat attttagttt
     1801 aatgtgcttt tctatattta gttcttttaa taaattatta taaagattgt ttatttcaag
     1861 attttcaatg tcatatgata agtcaataac caaactctta aactttaaat attttataaa
     1921 cctataaatt gcaagtaaaa taacaattgt tagatatatt attaatactg tatttgaata
     1981 atcaacttca ttcaaaatag gtttgtattg aaatttagaa tcagaaatag tataagtaaa
     2041 ataagagaat ggaaaaagta atctaactat tacaattagc caaatataat aattaaaacc
     2101 cttattgaaa attctgaata tagttgtcct taaaagtagc agaaaacaaa taactagact
     2161 acttgtgata ctggttctta aaatatctgt tagaattaaa ttagacatat atagtaccta
     2221 ttatttctta agttttttta taaaagctct caaagatgcc aattcatctt ttcctatctt
     2281 ttctttatta actgaatcaa ctaaacttac aatagaatca ttatgtacat tctttacaaa
     2341 atcagatgtt gcaaattcta agtagctctt tttatcaatc aaaattttat aagtagtgtg
     2401 tgtaggtttt tttgtaggct ctaaaaaccc tcttttagaa agcctagata atagtgtaag
     2461 tgttgtcgtt tgtttccatt cttcactcat tatatttaca atatctcttg atgtcaatac
     2521 cttgtcagta tcccaaaata gcatcattac tttaagttca gcacgtggta ttttttctat
     2581 cataatatgt tcctccatcc taatgttaaa aaaattaata aatgtaattt tattctattg
     2641 aaatagagta ggacagtcaa ttgttttttg attaatgatt tacatatgta gagtaaatta
     2701 aaagaagtat atcacttgaa aagttttaca tacgtagagc atttttatca aatctcttaa
     2761 tttttttaat tgaattatat atgtgaatat aagagtaaat caacttaaaa catgcaaaaa
     2821 attaatattt actgacatgc aactgacaaa tcaacaaaaa taggaattga taaaataagt
     2881 gtatatattg aaattgctat aatacataca aaaatggcat aaaatactaa tttttatgtc
     2941 cttttctact gacatgtaaa aaagtcttgt aagtattgga attactagat tacataggtt
     3001 ttagatgcaa aattgtcgtt ttgtaaaaaa ggctactaaa atttataaaa acaattatta
     3061 attgatataa attatcacaa aaaaagacat ctatttctat gaaaaaaaat tttcgtctgc
     3121 atgtaaaaat agctatagta atgcgtacga atgttctatt attacagaat tatttgtatg
     3181 taaattatat tgaaaatatc tatcagaatt agaattttat atggataaaa cagaactatc
     3241 aaataggagt atatagtaaa gatttatata atatgatgac ctcgtacaag cctgtaagag
     3301 ccgtttaaaa gttgctttag tattaattat ctttgaaaat ttaaaatagg tttaaatggc
     3361 ttgttaaata tgatgtatta ctattatgta taggggggta gttatgtagt tacatgttgt
     3421 tttaaaaaat aaatttaact atagttcttt cacttccaca ttacaaaaaa taaattgagt
     3481 gtgtatgttt ttaagtaaaa atgatgttta aatattgaaa aatagagata tagtgtatgt
     3541 aaaatttaga atagtgtatg taattttagg cataaaaata tgtgattaaa ccttaaaaac
     3601 caggaatata gtgtatgcat ttttaaaaat aaaaattgta acctttttgt aatcttttat
     3661 taaaatactt actttaagtc taaaaattag gggagatgta aggatttggg aaaaatagaa
     3721 gatgttataa tcataaatat ggtattcgta ggcttaaagt caaaaaggag gtgaaatata
     3781 aatagatttt tagctaaatt aagtaagaaa taggaggaga tttattgaac aaaaaattag
     3841 aaaaaccatt tgtatataag agagagtacg atttgactgg atatgatgtt gaaattttac
     3901 aaaaatatga gttagaacaa gcaatatatg tttatgttgg gagtagttgt gcatataaca
     3961 tgagagctag aagtagtaaa tggagatacc atataagaac aaataataag tctatatgtt
     4021 gtaacattaa aaattttata cataacttgg aattgtttta taaaatggaa ttaaagttgt
     4081 cagataatat tattaatgat aagctatact atagcaatat agcagagttt gaagaatttg
     4141 aaacactaga aaaagctaga gaggtagaaa gtactattat aagtcaatat caatttttag
     4201 attctataaa tcatatgtta aaacaaaaaa taattttatt gagtaataag gatagtgtgt
     4261 taaacataac taaaaatgga aatacaaatt atttgaaagt aaaaaataaa tacatagaaa
     4321 aacataagaa caagccaata atgagatacc atatcaactg tcaattcaat acagatggaa
     4381 gtgtcaaaag tattacacag gagtttgaac caatattgga attaaacaaa aaaaataccc
     4441 taagccgacc aagcagagta tttttaaaat aatattttaa gataacaaca aaatgagata
     4501 atactactag acaatgacaa ctcaactacc aattgagttt atggagctac caactccaat
     4561 atcggtctaa ctgattaagt atctgtagtt atataataat attgctatca attttagcat
     4621 cttaacaata ttattataca tactaagcta aaattattca atagttgtaa aagttgatta
     4681 gtcaataagt atatatttaa tgtagtgtta tctcttaaaa aaactagata aggagataat
     4741 aaatatatgg aacaattaga ttcaaaatat aagttgaaaa aatttctaat ggcagtattt
     4801 agagatggta taggacaagg aaataatctt attgataatg aatatgttag agtatttcaa
     4861 aataataaaa gtaatagtaa acaattagaa ctcggagaag aatttaaaga atatagtaaa
     4921 acaacttttt ttaaaaatat agatgatata gtagaattta ccttcgcaaa aaatatttat
     4981 tatgaaaata cattttttaa cctatgtaca actgatggaa aagcaggaac caatgaaaac
     5041 ttaataaata gatatgcatt aggatttgat tttgacaaaa aagaattagg acaaggtttt
     5101 aattataaag atataattaa tttatttact aagataggat tacattatca tatcctagtt
     5161 gatagtggaa atggattcca tgtttatgtg ctaattaata aaactaataa cattaagtta
     5221 gtatcagaag ttacaaatac attaataaat aaattgggtg cagataaaca agcaaattta
     5281 tctactcaag tattaagagt accttataca tataatatta aaaatactac taaacaagta
     5341 aaaataatac accaagacaa aaatatatat agatatgaca tagaaaagtt agctaaaaaa
     5401 tattgcaaag atgtaaaaac agtaggtaat actaatacaa aatatatatt agatagtaag
     5461 ctaccaaatt gtatagtaga tattttaaaa aatggtagta aagatggaca taaaaaccta
     5521 gatttgcaaa aaatagttgt gactttaaga ttgaggaata aaagtttaag tcaagtaata
     5581 tccgttgcta gagaatggaa ctatatatca caaaatagtc tttcaaatag tgagctagaa
     5641 tatcaagtca agtatatgta tgagaagctt aaaacggtta attttggttg tacaggttgt
     5701 gagtttaata gtgattgttg gaataaaata gaatcagatt ttatatatag tgatgaagat
     5761 actttgttca atatgccaca taagcactca aaggatttga aatataagaa taggaaaggg
     5821 gttaaaataa tgactggtaa tcaattgttt atctataatg tgttacttaa caataaagat
     5881 agagaattaa acatagacga tataatggag ctgataacct ataaacgtaa gaagaaagtt
     5941 aaaaacattg ttatgagtga aaagacatta agagaaacat taaaagaact tcaacataat
     6001 gattatatta caaaaacaaa aggtgttaca aagctaggaa taaaagatac atacaatgta
     6061 aaagaagtta gatgtaatat agataaacaa tatactatta gttactttgt taccatggca
     6121 gtaatttggg gaataatttc aactgaagaa ttaagattat atactcatat gagatataag
     6181 caagatttat tggtcaaaga tgataaaata aaaggaaata tattaagaat taatcaagag
     6241 gaattagcaa aagatttagg agtaacacag caaagaattt caaatatgat agaatcttta
     6301 ttagatacta aaattttaga tgtatgggaa actaaaataa atgatagagg atttatgtac
     6361 tatacatata gattaaacaa gtagattttt gataggatta gaattgattt tctagtccta
     6421 tttttatgca aaaaaactaa ttaataaaaa tttcttttgg taaaataatt gtacgagaat
     6481 tgcaaaaaaa aaatggcatc aaagtattga aattaagccg ttttaaaaat ttcttttggt
     6541 aaaataattg tacatatata tgtagtatat atatatatgt ttt
//
LOCUS       DBGJFF010000175         6481 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.179, whole genome shotgun sequence.
ACCESSION   DBGJFF010000175 DBGJFF010000000
VERSION     DBGJFF010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6481
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.179"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            351..1295
                     /locus_tag="ACOIAF_003530"
     CDS             351..1295
                     /locus_tag="ACOIAF_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HGZ2670510.1"
                     /translation="MKFLRIEDEVIYSKKRYKLKREIDKLFLDEEYFQIEPQLFEEYD
                     EFTTINNKIPEESMVKVVNGKVMVLRADITTSIIKSLVPRWEDGLKLKLFYNSSIYKN
                     KNTVGIKEIRQIGCEYLGEASVEADREVVKLALKILEKYNNNFILEVGSSKYIHGLLE
                     ELNLNKNCENQIKNLLYTKNTHELKVYIEELKIKHEVKELLSNILSLQGNLYNVIEKS
                     NKFYCNNKMKQALEELKQVNNLIEECNFLDKARFDLSMITMLDYYEGIMFRGYYPNSY
                     KEILSGGRYDSLTKEYGKEIPAIGFTLSVDELMKYVYK"
     gene            1317..1943
                     /gene="hisG"
                     /locus_tag="ACOIAF_003531"
     CDS             1317..1943
                     /gene="hisG"
                     /locus_tag="ACOIAF_003531"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HGZ2670511.1"
                     /translation="MDYLTIALAKGRIEGESFKKFKKMGLGDSIDTDTRKLIFKDEEN
                     KIIYIHVKPSDVVTYVEKGVADLGIAGKDTILENETDVYEIYDLGFGKCKFAVAGLKG
                     DSIYREDEYLKVATKYPNIAKKYFKEKGQKIEIIKLNGSVELAPIVGLSDVIVDIVET
                     GNTLKANGLEILEDICNISARIISNRASYRFKYEQIQNIIRLFEELDN"
     gene            1977..3026
                     /gene="hisC"
                     /locus_tag="ACOIAF_003532"
     CDS             1977..3026
                     /gene="hisC"
                     /locus_tag="ACOIAF_003532"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HGZ2670512.1"
                     /translation="MREKESIRELRGYEPNHVNCKVKLDANEGSKRLFKYLIKEISDS
                     DIDLNLYPEDSYSDLKESIIDYINISGVNKKNLLVGNGSSEIIDLIIHTFVDKDEVIL
                     SFSPSFSMYSIYSQINGSKFIGVESDENLVINIDNVIEKVKENNPKIVIVCNPNNPTG
                     TILKREEIIKLLDSTNSLVVLDEAYMDFGEESMLSDVFKYDNLIVLRTLSKAFGLAGI
                     RTGYMLSNSSLINSVEKVRPPYNLNSLSDFIATRALRNKDVVKAYIKEVKEEREVLYE
                     EMIGMGIKAYKSQANFILFYSEIENLSQKLIDRGVLIRKFGGKLENYYRVTIGDKEEN
                     SMFVGAIRDILKKEK"
     gene            3029..3607
                     /gene="hisB"
                     /locus_tag="ACOIAF_003533"
     CDS             3029..3607
                     /gene="hisB"
                     /locus_tag="ACOIAF_003533"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HGZ2670513.1"
                     /translation="MRIWKVERNTLETQILVELNIDGSGKAEIDTGIGFLDHMLTLMS
                     FHGKFDLKVICKGDTYVDDHHSVEDIGIAIGEAFKNALGDKKGIRRYSNIYIPMDESL
                     SMVAIDISNRPYLVFNAKFDTQMIGSMSTQCFKEFFRAFVNESRVTLHINLLYGENDH
                     HKIESIFKAFARALKEGSEIVSNEIASSKGVL"
     gene            3609..4214
                     /gene="hisH"
                     /locus_tag="ACOIAF_003534"
     CDS             3609..4214
                     /gene="hisH"
                     /locus_tag="ACOIAF_003534"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HGZ2670514.1"
                     /translation="MNIIVDYGLGNIDSVSRGFRKAGIETKISSDIDEIKQADSLILP
                     GVGAFRDSISALDKLGLIPIIKEHVSKGKFMIGICLGMQLLYEKSYEYGEYEGLGLIK
                     GSIDKLDISLKVPHMGWNNLKFNKANDDILKYINEDDYVYFVHSYYANSSNEELIAFS
                     EYEKKIPAIVRKGNVYGIQFHPEKSGEVGLNILRAYGEMIK"
     gene            4211..4933
                     /gene="hisA"
                     /locus_tag="ACOIAF_003535"
     CDS             4211..4933
                     /gene="hisA"
                     /locus_tag="ACOIAF_003535"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HGZ2670515.1"
                     /translation="MIIFPAIDIKDNKCVRLTQGEFDKVNVYYDNPLEVAYKWKNEGA
                     EYIHIVDLNGARSEFGVNTKIIEDIANNIDIPIQVGGGVRDKEKVKSLINAGVTRVIL
                     GSIAIENLNLVEELVNEYKEKIVVSIDAKDGKVAVRGWEVVSNVDSLTLCKQLEKIGV
                     QTIVYTDISKDGMLQGPNFDIYERIAKETSLNVIASGGVTSIEDVKRLKAMNLYGAII
                     GKALYDKKIDFKEAQQLCLLGE"
     gene            4915..5673
                     /gene="hisF"
                     /locus_tag="ACOIAF_003536"
     CDS             4915..5673
                     /gene="hisF"
                     /locus_tag="ACOIAF_003536"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HGZ2670516.1"
                     /translation="MLTRRIIPCLDVRNGRVVKGKKFKDIVDVDSPEVLGKFYSDCGA
                     DELVFYDITASNEERKTSLEFVTKVAENINIPFCVGGGVNKLEDFTDILRKGADKVSI
                     NSSAVKNPELIREASLKFGAQCVVLSIDAKKNEEGSWSVYVKGGREKTNLDAIEWAVK
                     GVELGAGEIVVNSMDEDGMKNGYDIELLSKITSLVNVPVIASGGAGKKEDFYEAVNKS
                     NVDGILAASVFHFGEIKINDLKKYLKDMGVEVRL"
     gene            5701..6369
                     /gene="hisIE"
                     /locus_tag="ACOIAF_003537"
     CDS             5701..6369
                     /gene="hisIE"
                     /locus_tag="ACOIAF_003537"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HGZ2670517.1"
                     /translation="MDNKCNNVYSDEVEKFIRSIKFDDKGLVPVVVQEVVSKDVLMLA
                     YMNKDAIKKTLKDKIACYFSRSRQELWVKGETSGNTQKVVKMSYDCDVDTILLFVEQT
                     GVACHTGNYSCFYRDLFDDSSKMELEVQTNILKELYDLINERKNNPVEGSYTNYLFEK
                     GIDKILKKVGEESSEVIIASKNTDKSELIYEISDLVYHTLVLMIEKGVEIDEIKKELL
                     KRRK"
BASE COUNT         2654 a          542 c         1264 g         2021 t
ORIGIN      
        1 ttaaataact attgccaatt tttgaaaaat atattattat taatagtaac aaaaaactgt
       61 aaatttagaa tattaataaa aattatttgg gaggaaatta tcatggtgag attaaatata
      121 gtaaaattaa atacaaaatt aaatcatcac catcatcata gattaaaggg attgtaatat
      181 gaaattaatt catagacaca agccctgttt ttagtgtaat tggtcttgta tctatgatgg
      241 gaagtaattt taagagaaaa ttatagccat ataggtataa agcctatgtg gcttttttat
      301 tgttttaaat ttgcaaatat aagagtaaat ttaatgaaga gggtgagaat gtgaaatttt
      361 tgaggattga agatgaggtt atttattcta aaaaaagata taaattaaaa agggaaatag
      421 ataaactttt tttggatgaa gaatattttc aaatagaacc acaattattt gaagaatatg
      481 atgaatttac aactataaat aataaaattc cagaagaatc tatggtaaag gttgtaaatg
      541 gaaaagtaat ggtacttaga gctgatataa ctacaagtat aataaaaagc cttgtaccaa
      601 gatgggaaga tggtttaaag cttaagcttt tttataattc atcaatatat aaaaataaaa
      661 atacagttgg aataaaagag ataagacaaa taggttgtga gtatctagga gaagcctctg
      721 tagaggcaga tagagaagta gtgaaattag ctcttaagat acttgaaaaa tacaataata
      781 attttattct agaggttggg agtagcaagt atattcatgg tttgttagaa gaattaaatt
      841 taaataagaa ttgtgaaaat caaattaaaa atttactata tacgaagaac actcatgaat
      901 taaaagtata tattgaagaa ttgaaaatta agcatgaagt aaaagagtta ttatcaaata
      961 ttttaagttt acaagggaat ttatataatg ttattgaaaa atcaaacaag ttctattgta
     1021 ataataagat gaagcaagct cttgaagaat taaagcaagt aaataattta attgaggaat
     1081 gtaatttttt agataaggct agatttgatt tatcaatgat tactatgctt gactattatg
     1141 aaggaattat gtttagagga tactacccaa actcatataa agaaattctt agtggtggaa
     1201 gatatgattc tttaactaaa gagtatggaa aagaaattcc agccattgga tttacactaa
     1261 gtgttgatga gttaatgaag tatgtatata agtaaagttt agaaggaggg attgctttgg
     1321 attatttgac tatagcacta gcaaaaggac gaattgaagg ggaatcattt aaaaaattta
     1381 aaaaaatggg tctaggagat agtatagaca cagatacgag aaaactaatt tttaaagatg
     1441 aagaaaataa aattatatat atacatgtaa agccatcaga tgtagttaca tatgttgaaa
     1501 aaggagtcgc tgacctcggt atagcaggta aagatactat tttagaaaat gagacagatg
     1561 tatatgaaat atatgattta ggttttggaa aatgtaaatt tgctgtagca ggattaaagg
     1621 gagatagcat atatagagaa gatgaatatt taaaagtggc aacaaagtat cccaatatag
     1681 caaaaaagta ttttaaagag aaaggtcaaa aaattgaaat aataaaatta aatggttctg
     1741 tagaactagc tccaatagta gggttgtcag atgtaattgt agatatagtt gagactggca
     1801 atacacttaa agcaaatggt cttgaaatat tagaagacat atgtaatata agtgctagaa
     1861 ttatatcaaa tagagctagc tatagattta aatatgaaca aattcagaat ataattcgtt
     1921 tatttgaaga acttgataat taaaatataa ttgagcttta taaggaggga tttaagttga
     1981 gagaaaaaga aagcataaga gagttaaggg gatatgaacc aaatcacgta aactgtaaag
     2041 taaaacttga tgcaaatgaa ggaagtaaga ggttgtttaa ataccttatt aaagaaattt
     2101 cagatagtga tatagattta aatttatatc ctgaagattc ttatagtgat ttgaaagaat
     2161 ctataataga ttacataaat atttctggag taaataagaa aaatcttcta gtaggaaacg
     2221 gttcaagtga aataattgat ttaataatac atacttttgt agataaagac gaagtaatat
     2281 tgagtttctc tccaagtttt agtatgtatt ctatatatag tcagataaat gggtctaaat
     2341 ttataggtgt tgaaagtgat gaaaatttag ttataaatat agataatgtg attgaaaaag
     2401 taaaggagaa taatcctaaa atagtaattg tatgtaatcc taataatcca actggaacaa
     2461 tattaaagag ggaagagata ataaagcttt tagattcaac taatagtcta gtggttttag
     2521 atgaagcata tatggatttt ggagaagaaa gcatgttaag tgatgttttt aagtatgata
     2581 atctaatagt tcttagaact ttatcaaagg catttggatt ggctggaata agaacaggat
     2641 atatgttatc taatagtagt ttaataaatt ctgttgagaa agtaagacct ccatataatt
     2701 taaattcatt atcagacttt attgctacaa gagcacttag aaataaagat gtggtaaaag
     2761 cttatataaa ggaagtaaaa gaggaaagag aagttttata tgaagaaatg attggtatgg
     2821 gaattaaagc atacaaatca caagcaaatt ttatattatt ttattctgaa atcgaaaatt
     2881 tatcacaaaa acttattgat agaggagttt taataagaaa atttggtgga aagttagaaa
     2941 attattacag agttacaatt ggtgataaag aagaaaattc tatgtttgtt ggagctataa
     3001 gagatatttt gaaaaaggag aaataggtat gagaatttgg aaggtagaaa gaaatactct
     3061 tgaaacacag attttagtag aattaaatat tgatggttct ggtaaagctg aaatagatac
     3121 tggaatagga tttttagacc atatgcttac cttaatgtca tttcatggca agttcgattt
     3181 aaaagtgatt tgtaagggtg atacatatgt agatgaccac catagtgtag aagatatagg
     3241 tatagctata ggtgaagcat ttaaaaatgc tctaggtgac aaaaaaggaa tacgaagata
     3301 ttctaatatc tatataccta tggatgaatc tctatcaatg gtagcaatag atataagcaa
     3361 tagaccttat cttgtattta atgctaaatt tgatacccaa atgattggta gtatgagtac
     3421 tcaatgcttt aaagaatttt ttagagcttt tgtaaatgaa tcgagagtaa cattacatat
     3481 caatctctta tatggggaaa atgaccatca taagatagaa agtattttta aagcatttgc
     3541 aagagcttta aaagagggta gtgaaatagt ttctaatgag atagcatctt caaagggggt
     3601 tttgtagaat gaatataata gtagactatg gtcttggaaa tatagattct gtgtctaggg
     3661 gttttagaaa agctggaata gaaactaaaa tttctagtga tatagatgaa ataaaacagg
     3721 cagattcact tatacttccg ggtgttggag cttttagaga ttctataagt gccttagata
     3781 aactaggatt gataccaata ataaaggagc atgtttctaa gggtaaattt atgataggta
     3841 tatgtctagg aatgcaatta ctttatgaaa aaagttatga gtatggagaa tatgaaggat
     3901 taggacttat aaaaggaagt attgataaac tggatataag tttaaaagtg ccacatatgg
     3961 gatggaataa tctaaaattt aataaggcaa atgatgatat acttaaatat ataaatgaag
     4021 atgattatgt atacttcgtt cactcatatt atgcaaattc atcaaatgaa gaactaattg
     4081 cattttcaga atatgaaaag aaaatccctg ctattgttag aaaaggcaat gtatatggta
     4141 tacaatttca tcctgaaaaa agtggagaag tagggttaaa tatattaaga gcatatgggg
     4201 agatgataaa atgataatat ttccagcaat agatataaaa gataataagt gtgtaagact
     4261 cacacaagga gaatttgaca aagtaaatgt atattatgat aatccattag aagtagcata
     4321 taaatggaaa aatgaaggag cagaatatat acatatagta gatttaaatg gtgctagaag
     4381 tgaatttggt gtaaatacaa aaataattga ggacatagca aacaatatag atatacctat
     4441 tcaagttggc ggtggagtta gggataagga aaaagtcaaa agtttaataa atgctggtgt
     4501 tactagagta atattaggta gtatagcgat tgaaaatttg aatttagttg aagaattggt
     4561 caatgaatat aaagagaaaa ttgtagtttc tatagatgct aaagatggaa aagtagcagt
     4621 taggggatgg gaagttgtaa gcaatgtaga ctcattaaca ttatgtaaac aacttgaaaa
     4681 gataggtgta caaactattg tttatacaga tatatcaaaa gatgggatgt tacaaggtcc
     4741 aaattttgat atatatgaaa gaatagcaaa ggaaacatca ctaaatgtta ttgcttctgg
     4801 aggcgtaact tctatagaag acgtaaaaag actaaaagct atgaacttat atggtgcaat
     4861 aataggtaaa gcgctttatg ataaaaagat agactttaag gaggcacaac aattatgctt
     4921 actaggagaa taataccttg tctagatgtg agaaatggaa gagttgtaaa ggggaaaaaa
     4981 tttaaagata tagtagatgt tgatagtcca gaagtacttg gaaaatttta tagtgattgt
     5041 ggtgcagatg aacttgtatt ctatgatata actgcatcaa atgaagagag aaagacatct
     5101 ttagaatttg tgacaaaagt tgcagaaaat ataaatatac cattttgtgt tggtggagga
     5161 gtaaataaat tagaagattt cactgatatt ttaagaaaag gtgctgataa agtttctata
     5221 aattcatcag cagtcaaaaa tcctgaatta ataagggaag cttcattaaa atttggagca
     5281 caatgtgttg ttttatcgat agatgcaaag aaaaatgaag agggttcatg gagcgtatac
     5341 gtaaaaggtg gaagagaaaa aactaatctg gatgcaattg aatgggctgt taaaggtgta
     5401 gaacttggag caggagaaat cgttgtaaat agtatggatg aagatggaat gaaaaatgga
     5461 tatgatatag aattattatc aaagataact tctttagtaa atgtacctgt tatagcatct
     5521 ggaggcgctg gaaaaaagga agatttttat gaggcggtta ataaatccaa tgtagatgga
     5581 attttagcag catctgtttt tcattttggc gagataaaaa taaacgattt aaagaaatat
     5641 ttaaaagaca tgggtgtaga agtaagatta taagttaaat actaaaagga gattagaaat
     5701 atggataata agtgtaacaa tgtatacagt gatgaagtag agaaatttat aagaagtata
     5761 aagtttgatg ataaagggtt agtccctgtt gttgttcaag aggtagttag caaagatgta
     5821 ctgatgttag catatatgaa taaagatgct ataaagaaaa ccttaaaaga taaaatagct
     5881 tgctatttta gtagaagtag acaagaactt tgggttaaag gtgagacatc tggtaatact
     5941 caaaaagtag ttaagatgtc ttatgattgt gatgtagata ctatactact ttttgttgaa
     6001 caaactggtg tagcttgtca tacaggcaat tattcttgtt tttacagaga cttgtttgat
     6061 gatagttcaa aaatggagct tgaagtacaa acaaatatat tgaaagaatt atatgattta
     6121 atcaatgaaa gaaaaaataa tccagttgaa ggttcttata ctaattatct ttttgaaaaa
     6181 ggaatagata agattcttaa aaaagtaggt gaagagagta gtgaggtaat tatagcatca
     6241 aaaaataccg ataaaagtga acttatttac gaaattagcg atttagtata tcatacattg
     6301 gttttaatga tagaaaaagg tgttgaaatt gatgaaataa aaaaagaatt acttaaaaga
     6361 agaaaataga ataagataga ttaatgggta ataagaaaat ataccgcttt taataaaaag
     6421 tggtatattt tttattttat attttaattt atagaataat atttaaatta cttgataaaa
     6481 t
//
LOCUS       DBGJFF010000176         6411 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.180, whole genome shotgun sequence.
ACCESSION   DBGJFF010000176 DBGJFF010000000
VERSION     DBGJFF010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6411
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.180"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            241..1062
                     /locus_tag="ACOIAF_003538"
     CDS             241..1062
                     /locus_tag="ACOIAF_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HGZ2670518.1"
                     /translation="MNLIDNHIHTNFSSDGKDSMEDTIKKAISIGVRYLTFTDHLEHD
                     EDRGFCINYNDYVPVFNKFKEKYKKDIELLLGVEVGYRKHLKNEIEEIINSNPFDFVL
                     CSTHTIDNVPVPSKAYFKGLSKEDAYYKYFNSILETNREFGDYNIYGHLDYISRYGIY
                     SDNRVIYNDFKDVIDEVLKSIINNGSGIELNTSGYRYGLNAIHPNEDILKRYKELGGF
                     IVTVGSDSHRVEDICKDFDVAYDMLKYLDFKYVSLFKERETYFVNIEKVKSNNIA"
     gene            1320..3203
                     /locus_tag="ACOIAF_003539"
     CDS             1320..3203
                     /locus_tag="ACOIAF_003539"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HGZ2670519.1"
                     /translation="MSNKNKENITLSNQIKGTIQNSLTKEAILHIPMSNYAYGYDRET
                     LHIRLRTKKNEAKRVILRIGDQYVWDKGGAGGGNLNASGVGWSGGTNIVMSKEVETEL
                     FDYWIAKCKPLNKRSRYGFIIEGQEEKLLFTEKRIIELGNENDEKELCEIGNFFGYPY
                     LNYIDIPKVPNWVKDTVWYQIFPDRFANGDPSINPEGVDKWGAIPTRDNFTGGDLQGV
                     IEHLDYLSDLGINGIYFCPITIGKTNHRYDTVDYMEVDPTLGDKETLKKLIEEAHKRN
                     IKIMLDAVFNHIGYYSKQWQDVVQNKENSRYKNWFYIKDMSKIDTPIEQIDEKNIPYE
                     TFGCEKYMPKLNTENSEVIKYLLDVGKYWIQEFDIDAWRLDVSNEVDHVFWRKFREEV
                     KKVKPDIYILGEIWHGSLPWLMGDQFDSVMNYLMSEAMKKFFCTDEINAEEFKYMIND
                     VMVSYPIQVSEVIFNLLGSHDTTRILTYANGNIDRFKLAYMFMFVQTGCPCIYYGDEI
                     GMEGELSSISEGQRKCMEWNEEKWNKDIFDFMKKIIRLRKENKELRSISNEWVLADKE
                     NGTIILKKESISIIINNSSKNIILTLPDYLANKKVKDLYEEEIVDLKEELMLEKYKFI
                     ILK"
     gene            3427..4107
                     /gene="pgmB"
                     /locus_tag="ACOIAF_003540"
     CDS             3427..4107
                     /gene="pgmB"
                     /locus_tag="ACOIAF_003540"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HGZ2670520.1"
                     /translation="MIEAFIFDLDGVITDTAYYHYMAWRKLAHKVGIDIDTKFNESLK
                     GISRMESLDRILEFGNKKYSFSEEEKVRMAEEKNNYYVSLIDEITSNDILPGIESLLI
                     DVKSNNIKIGLSSASKNAINVLNHLGISDKFDFIADAGKCKNNKPHPEIFLMSAKGLN
                     VNPQNCIGIEDASAGIDAINSANMFSVGVGNYENLKKANLVVDSTNQLKFEYIQEKYN
                     EYIVRRII"
     gene            4104..6389
                     /locus_tag="ACOIAF_003541"
     CDS             4104..6389
                     /locus_tag="ACOIAF_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HGZ2670521.1"
                     /translation="MKCLFSNVDEWKIIQDKIEIKENRLAESIMSIGNGYMGMRGNYE
                     ENYSGDTHKGSYIAGVWFPDKTRVGWWKNGYPEYFGKIPNSVNYIGVRIFIDDVELDL
                     AKCKVEDFYRELDMKNGILNRRFIVNINDKKFEVRVARFLSISVKELAVIKYSIKSLN
                     SNTKIKFVPYLDSNIKNEDSNYEEKFWNGISSSSLEYKGSILSRTRENAFNTPVFTVG
                     SMMYIKTNGDVEAISHESDYNYCENSITMDVLKNEVASIEKYVSITSTRDYKESELLR
                     KCEDILNRELSKSYDDILNEHIKLWNKRWESADVKINGDKSSQQGIRYNLFQLFSTYY
                     GEDSRLNIGPKGFTGEKYGGATYWDTEAFCVPVYLGVADKEVTRNLLEYRYNQLEQAK
                     DNAKKLGLKGALYPMVTFDGVECHNEWEITFEEIHRNGSIVYAIYNYTNYTGDYKYIK
                     EKGIDVIIEIARFWASRVHLSTKKDLYMIHGVTGPNEYENNINNNWYTNYIAKWCLDY
                     AVENILRLEKECNECIDRNNVTREEVEFWKIISNKMYLPYDEELQIIIQNDNFLDKEF
                     VEVKDLPKENFPLNQNWSWDKILRSCFIKQADVLQGIYYFGNRFTKEEKKRNFDFYEK
                     YTVHESSLSSSIYSIIASEIDNLEKAYELYSRTARLDLDNYNNDTNDGLHITSMSGSW
                     LSIVHGFAGMRTWNETLSFDPKLPKEWKNYSFNINYRDNIINVDVCSDLIKIENLKGK
                     PIKIIVYGKKYILEKCIKIDK"
BASE COUNT         2641 a          564 c         1163 g         2043 t
ORIGIN      
        1 gacatataac agctaaattc tattacaaaa ttaagacttt taaaaattaa attgaagtat
       61 ataaaagtaa cattaggaga agtatcctta atgattttct aaaatcagaa ataggtgctt
      121 ctcttttttg taaaaataaa agtatgtaaa taaaatttaa ccattataat tttcttttaa
      181 caaatgtata caataaactt gttgtataat taagatagat aaaataaagg gagggataat
      241 atgaatttaa tagataatca tattcatact aatttttcaa gtgatggaaa ggattccatg
      301 gaagatacaa taaaaaaagc tataagtatt ggtgttagat atttgacttt tacagaccat
      361 ttagaacatg atgaagatag aggattttgc ataaattata atgactatgt tccagtattt
      421 aataagttta aagaaaaata taaaaaggac atagagttat tactaggtgt tgaagtgggt
      481 tacagaaaac atctaaaaaa tgaaatagaa gaaataataa attctaatcc ttttgatttt
      541 gtattgtgct caactcatac aattgacaat gtacctgttc catcaaaggc gtattttaaa
      601 ggtcttagca aagaagatgc gtattataaa tattttaata gcatacttga aactaatcgt
      661 gaatttggag attacaatat atatggtcat ttggattata tatctaggta tggaatatat
      721 tcagataata gagttatata caatgacttt aaagatgtta ttgatgaggt cttaaagtca
      781 ataatcaata atgggagtgg tatagagcta aatacttctg gttatagata tggattaaat
      841 gctatacatc caaatgaaga tattctaaaa agatataaag aacttggagg atttatagtt
      901 actgtaggtt ctgattctca tagggtagaa gatatatgta aagattttga tgtggcctat
      961 gatatgctta agtatcttga ctttaaatat gtatctttat ttaaagaaag agaaacttat
     1021 tttgtaaata tagaaaaagt taaatctaac aatatagctt agagtgtgtt ttgaagtaaa
     1081 ttaatggata gttaaaaagt taatagttat aatattgtat cctctagaaa aatactagag
     1141 gtttttattt tgctaaaata actaaataga attaaattta aatagtatag tttattttaa
     1201 attgaatagt ttattgttga tatattttac tattatatat ttttattgtg atataatagt
     1261 aaaatatatt aacctatctt tagattatat aaattattat aaaggaggga aattttaaaa
     1321 tgtctaataa aaataaagaa aatataactt taagtaatca aatcaaagga acaattcaaa
     1381 atagtttaac aaaagaagct atattacata taccaatgag caattatgcc tatggttatg
     1441 atagagaaac actacatata agacttagaa ctaaaaaaaa tgaagcgaaa agagtaattt
     1501 tacgaatagg agaccaatat gtgtgggata aaggaggtgc aggaggagga aatttaaatg
     1561 catctggagt tggttggtct ggtggaacaa atatagtaat gagtaaagaa gtagaaactg
     1621 aattatttga ctattggata gctaagtgta agcctttaaa taaacgctca cgatatggct
     1681 ttataataga aggtcaagag gaaaaacttt tatttacaga aaaaagaata atagagttgg
     1741 gaaatgaaaa tgatgaaaag gaattatgtg aaataggtaa tttttttgga tatccatatt
     1801 taaattatat agatatacct aaagtgccaa attgggtgaa agatactgtt tggtatcaga
     1861 tatttccaga tagatttgca aatggagacc cgtctataaa cccagaagga gtagataaat
     1921 ggggagctat tccaaccaga gataatttta caggtggaga tttacaaggt gtgatagaac
     1981 acttagatta tttgagtgat ttgggaataa acggaattta cttttgccct attacaattg
     2041 gaaaaaccaa tcatagatat gatacagtag attatatgga ggtagaccct acattaggag
     2101 ataaagagac tttaaaaaaa ctaatagaag aagcgcataa gagaaatata aaaataatgt
     2161 tggatgctgt ttttaatcat ataggatatt attctaaaca gtggcaagat gtagttcaaa
     2221 ataaggaaaa ttctcgatat aaaaactggt tttatataaa agatatgagt aaaattgata
     2281 ctccaataga acaaatagat gaaaaaaata taccttatga aacctttggg tgtgaaaaat
     2341 atatgcctaa acttaataca gaaaattcag aagtaataaa atatttattg gatgtaggca
     2401 agtactggat acaggaattt gatatagatg cgtggagatt agatgtttca aatgaagtag
     2461 accatgtttt ttggagaaag tttagagaag aagtcaaaaa agtaaaacca gacatttata
     2521 tattgggaga aatttggcat ggtagtttac catggttaat gggtgaccaa tttgattcag
     2581 ttatgaatta cttgatgtca gaagccatga aaaaattctt ttgtacagat gagataaacg
     2641 ccgaagaatt taagtatatg ataaatgatg taatggtaag ttatccaata caagttagtg
     2701 aagttatttt taatttactt ggaagtcacg atactacaag aatattgact tatgctaatg
     2761 gaaatattga tagatttaaa cttgcatata tgtttatgtt tgtacaaaca ggatgtcctt
     2821 gtatatacta tggtgatgaa atcggaatgg aaggagaatt aagttcaata tctgaaggtc
     2881 aaagaaaatg tatggaatgg aatgaagaaa aatggaataa ggatatattt gattttatga
     2941 agaagattat tagacttcgt aaagaaaata aagaacttag gtctatatca aatgaatggg
     3001 tgttagcaga taaggaaaat ggaacgatta tattgaaaaa agaatctatt tctataataa
     3061 taaataattc atctaaaaat ataattttga cgttaccaga ttatttagca aataaaaaag
     3121 taaaagattt atatgaagaa gaaatagttg atttaaaaga agaactaatg ttagagaaat
     3181 acaagttcat tattttaaaa taatacaaaa aagtatttat tttagatttc atgttaaatt
     3241 attatacagt ttaaacaagt ctttataaac gataaaaaat atggtctaca agtgagtgaa
     3301 ttgaaagtag attttaaatt aaagactgag ttagagtaga taattttata aataatatct
     3361 taaatacaag atagaaaata aattgattat tagtctaaaa atttcttgta gaaagaggaa
     3421 ataagaatga ttgaagcatt tatatttgat ttagatggtg taattacaga tactgcatat
     3481 tatcactata tggcttggag aaaactagct cataaagttg ggatagatat agacacaaaa
     3541 tttaatgaat ctttaaaagg cataagtaga atggagtcat tggacaggat attagaattt
     3601 ggaaataaga aatattcatt ttcagaagaa gaaaaagtga gaatggctga agaaaaaaat
     3661 aattactatg tatcattgat agatgagata acttcaaatg atattttgcc aggcatagaa
     3721 agcttgttaa ttgatgtaaa atctaataat ataaagatag gtctatcttc tgcaagtaag
     3781 aatgcaataa atgtattaaa tcatttgggt ataagtgaca aatttgattt tatagctgat
     3841 gcaggaaaat gtaaaaacaa taaaccacat ccagagatat ttcttatgtc agcgaaaggg
     3901 cttaatgtaa atccacaaaa ttgcataggg atagaggatg caagtgcagg tatagacgct
     3961 ataaactcag ctaatatgtt ttctgttggt gttggaaatt atgaaaactt aaaaaaagca
     4021 aatttagttg tagattcaac aaatcaatta aagtttgaat atatacaaga aaagtataat
     4081 gagtatatag ttaggagaat tatatgaaat gtttatttag taatgtagat gagtggaaga
     4141 ttatacaaga taagattgaa atcaaagaaa atagattagc agagtccata atgagtatag
     4201 gcaatggata tatgggtatg agaggaaact atgaggaaaa ttatagtgga gatacacaca
     4261 aggggtctta tatagctgga gtatggtttc cagacaaaac tcgtgttggt tggtggaaaa
     4321 atgggtatcc agaatacttt ggtaaaattc ctaattcagt caactatata ggtgtaagaa
     4381 ttttcataga tgatgtagaa ttagatttag caaaatgtaa agtagaagat ttttatagag
     4441 aattagatat gaaaaatggt attttaaatc gtaggtttat tgtaaatata aatgataaaa
     4501 aatttgaagt tagggttgct aggtttttaa gtatatctgt taaggaactt gcagtaatta
     4561 aatacagtat aaaatcatta aattctaata ctaaaataaa atttgtacca tatttagatt
     4621 caaatataaa aaatgaagat tcaaactatg aggaaaagtt ttggaatgga atttcttcaa
     4681 gttctttaga atataaaggt agtattcttt ctcgaacaag ggagaatgca tttaatacac
     4741 cagtattcac tgtgggttca atgatgtata ttaagacaaa tggtgatgta gaagctatat
     4801 ctcatgagag tgattataat tactgtgaaa attcgataac aatggatgtt ttaaaaaatg
     4861 aggttgcaag tatagaaaaa tatgtatcta taacctcaac tagagattat aaggaaagtg
     4921 agctattaag aaaatgtgaa gacattttaa atagagaact atctaaaagt tatgatgata
     4981 tattaaatga acatataaag ttatggaata aaagatggga aagtgcagat gtaaaaataa
     5041 atggagataa aagtagccaa caaggtataa gatataattt attccaacta ttttcaacat
     5101 attatggaga agattcgcga ttaaatatag gtccaaaggg atttactggt gaaaagtatg
     5161 gaggagctac ttattgggat acagaagcgt tttgtgttcc agtatatcta ggtgtagcag
     5221 ataaggaggt aactagaaat cttttagaat ataggtacaa tcagttagaa caggcaaagg
     5281 ataatgcaaa aaaactaggg cttaaaggtg ctttatatcc aatggtaaca tttgatggag
     5341 tagaatgtca taatgaatgg gagataacat ttgaggaaat ccaccgaaat ggaagtatag
     5401 tatatgcaat atataactat acaaattata caggtgacta taaatatata aaagaaaaag
     5461 gaatagatgt aataatagaa atagctagat tttgggcaag tagagttcat ttgtctacaa
     5521 aaaaagattt gtatatgata catggagtaa caggtccaaa cgagtatgaa aacaatatca
     5581 ataataattg gtatactaat tatatagcta aatggtgttt agattatgct gtagaaaata
     5641 tcttacgatt agagaaagag tgtaatgaat gtattgacag aaataatgtg actagggaag
     5701 aagttgagtt ttggaaaata atatcaaata aaatgtatct gccgtatgat gaggaactac
     5761 aaataataat tcaaaatgat aattttttag ataaagaatt tgtagaagta aaggatttac
     5821 caaaagaaaa ttttcctctt aatcaaaatt ggtcttggga taagattctt aggtcgtgtt
     5881 ttataaaaca agcagatgta ttacagggaa tttactattt tggaaataga tttactaaag
     5941 aagaaaaaaa gagaaatttt gacttctatg aaaaatatac tgtccatgaa tcttctttat
     6001 cttctagtat ttattctatt attgcctcag aaatagataa cctagaaaag gcttatgaat
     6061 tatattcaag aacagctagg cttgatttag ataattacaa taatgataca aatgatggac
     6121 tgcacataac ttcaatgtct ggttcatggc tttccattgt acatggattt gcaggcatga
     6181 gaacatggaa tgaaactcta tcttttgacc ctaagttgcc aaaagagtgg aaaaattatt
     6241 ctttcaatat aaattataga gataatatta ttaatgtaga tgtatgtagt gatttaatta
     6301 aaatagaaaa tttaaaaggt aaaccaataa aaattattgt atatgggaaa aaatatatat
     6361 tagaaaaatg tataaaaatt gataaataga tttaataata tttttattat a
//
LOCUS       DBGJFF010000177         6377 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.181, whole genome shotgun sequence.
ACCESSION   DBGJFF010000177 DBGJFF010000000
VERSION     DBGJFF010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6377
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.181"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(66..623)
                     /locus_tag="ACOIAF_003542"
     CDS             complement(66..623)
                     /locus_tag="ACOIAF_003542"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HGZ2670522.1"
                     /translation="MTEKEKMLSGKGYYANDELLVKEREYCKKLTRLFNNTLEDEYEK
                     REDILRQLFGSVGKQINVEQNIRCDYGYNIHVGENFFANYDCIFLDVCKIEIGDNVML
                     APNVQIYTAYHPIDAQLRNSGIEYGSPIKIGDNVWIGGGVIITPGITIGDNVVIGAGS
                     VVTKDIPPNTVAVGNPCRVIKKIEE"
     gene            complement(629..1705)
                     /locus_tag="ACOIAF_003543"
     CDS             complement(629..1705)
                     /locus_tag="ACOIAF_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HGZ2670523.1"
                     /translation="MSLYVDIEKDLSSFKLKVEIKQEKGTLGFLGESGSGKSMTLKCI
                     AGLEKPTRGKIVLNDRVLFDSEKKINLSTQDRKVGFLFQNYALFPHMTVSQNIELGLL
                     KLSKSEKKEIVARYLDILKLNGFEGRYPWQLSGGQQQRVALARALATSPDILLLDEPF
                     SALDHHLRSNMEKELMNMLKDYKGDILFVTHDIEEAYRVCDDIIVYNKGEGLPKRPKK
                     ELFESPKYLIEAKITGCKNISKLNRLDKNTIYATDWGCELTLNREIGDNIEYVGIREH
                     HIKVLDSNEDLNEKLCFELINIVENPFTYTIYVRKTDLSNECVPIQIELEKSKMRFKK
                     GDRIYLDFPQEYLFCFRYNYNKKE"
     gene            complement(1706..2389)
                     /gene="modB"
                     /locus_tag="ACOIAF_003544"
     CDS             complement(1706..2389)
                     /gene="modB"
                     /locus_tag="ACOIAF_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HGZ2670524.1"
                     /translation="MGTDWSPLWISLKTSILSTIITFVIGIFVSYIMANYRGKWKGLI
                     DGLFTLPLILPPTVVGFFLLLLCGKNGFIGKFLLNFDRTLIFSWTATVISAVVVSFPM
                     MYRTSRSAFEQIDNNMLSAARTLGLNEWKVFFKIAVPLAWPGIIGGLVLSFARALGEF
                     GATLMIAGNIPGKTQTMPIAIFFAVEGGDMNKAMMWVMIIVAISIAMILLLNYWSEFQ
                     QKIIGKRCG"
     gene            complement(2538..3350)
                     /gene="modA"
                     /locus_tag="ACOIAF_003545"
     CDS             complement(2538..3350)
                     /gene="modA"
                     /locus_tag="ACOIAF_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HGZ2670525.1"
                     /translation="MKKILGILGLVACLTLGTVGCNSNEDKKDDGKQEKTTKSSDSVE
                     LNISAAASLKEAMAKIEEEYKKVDSNVKLTVNYGASGSLQQQIEQGAPCDLFISAGQK
                     QMKALDEEKLLVSDTMKDLVKNDLVLISSADSSVSGMKDLTTDKVKKIAVGEAESVPA
                     GKYADEVLTNLNLKDKLKDKLVFAKDVKEVLAWVQSGNADVGFVYFSDTVNNDKIKVV
                     EKTDEKTHSPITYPVSVIKASKNVDAAKKFEEFLLSESGQKIFEEFGYKKVE"
     gene            3543..4472
                     /locus_tag="ACOIAF_003546"
     CDS             3543..4472
                     /locus_tag="ACOIAF_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HGZ2670526.1"
                     /translation="MDSLSSLTPVEVAELLKITKNTVYELIKRGELPSYKVGKKIRVD
                     TKDVEEYINSQKTGKVKDTHFNNKNIDINNNFKIIISGQDVILDILARSVEKKLDGIS
                     TFRSYIGSYNALYELYNGRVSIASSHLWDFETDEYNSTFVKKLLPGIPCVLINLAYRM
                     QGFYVAKGNPKEITTWEDLTKPSITIVNREKGSGTRVLLDGKLRLLNFNGKHIKGYDN
                     EELSHLGVASTVSRGIADVGLGNEKAALQVNNIDFIPLHKERYDLVIKKEDLQNPVYQ
                     TIINIINSPEFKAELQGLGGYDLTDTGKTIAKV"
     gene            complement(4633..5082)
                     /locus_tag="ACOIAF_003547"
     CDS             complement(4633..5082)
                     /locus_tag="ACOIAF_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670527.1"
                     /translation="MKNKNNKDKNMLNIKVLRVLGVLLILFDYTGDTLNFYNFFAKPL
                     VSGNLYKTTGTDCIVFLLFIVECVYLVGRYILKVKINWSEIIENEKGNFINNLLVEKI
                     QEESNIVYNLLNSYVLPHIYKKARGLMFKTYHMLIYIKEKYYYVNIY"
     gene            complement(5285..6157)
                     /locus_tag="ACOIAF_003548"
     CDS             complement(5285..6157)
                     /locus_tag="ACOIAF_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGZ2670528.1"
                     /translation="MYNSSVGLEQLIKNEDIVKIFSERKDRPLEKLKNYKTSELMIQS
                     SNNYKLESLFITSNIKTRDTMILVHGIGSSYYEMLKVAYRYLDKGYNVLVYNQRNTGN
                     SGGDNYTFGLYERYDLDSLVKFVKNKFPEGRLGVHGFSMGAGTAAMHSEINSKDDKVD
                     FYILDSPYSEMKDAIRMGVLEKRIPDILINYVVTCGDLYNKFKSGFWYSDVKPYESVE
                     KSNVPILFIHGTKDTVCNYQNSKKMYDLVKHDKKDLWLIEGIGHVDGFEHDSTVYFNK
                     IFKFIDSNVLSDKS"
BASE COUNT         2139 a         1112 c          674 g         2452 t
ORIGIN      
        1 ttatatgtaa atgctcttaa tttgtttgta taaattttac ttgtataaac tttatacaat
       61 catatttatt cttctatttt ctttattact ctacatggat ttccaacagc tacagtattt
      121 ggtggtatat cttttgtgac tacacttcct gcacctatta ctacattatc acctatagta
      181 attcctggtg ttatgattac tcccccacct atccatacat tatcacctat ttttatagga
      241 gaaccatact ctatgcctga atttctaagt tgtgcatcaa ttggatgata ggctgtatag
      301 atttgaacgt ttggagctaa cattacattg tcacctattt caattttaca tacatctaaa
      361 aaaatacaat cataatttgc aaagaaattt tcgcctacat gtatgttata tccataatca
      421 cacctaatat tttgttctac atttatttgc ttgccaactg aaccaaatag ttgtcttaaa
      481 atatcttctc ttttttcata ttcatcctct aaagtattat taaacaatct agttaatttt
      541 ttacaatact ctctttcttt tactaacaat tcatcatttg catagtatcc tttcccagac
      601 aacatttttt ctttttcagt catatagttt actccttttt gttataattg tatctaaagc
      661 aaaataaata ttcctgtgga aaatctaaat atattctgtc acctttctta aatctcattt
      721 tacttttttc caattctatt tgaataggta cacattcgtt agataaatca gtttttctaa
      781 catatatagt atatgtaaaa ggattttcta caatgttaat caattcgaaa cataactttt
      841 catttaaatc ttcattagaa tccaagacct taatatgatg ctccctgatt cccacatatt
      901 ctatattatc acctatttct ctatttaatg tcaactcgca tccccagtct gtagcataaa
      961 tagtattttt atccagtcta tttaatttgg atatattttt acaccctgtt atcttagctt
     1021 ctataaggta ctttggagat tcaaagagct cttttttagg tctctttgga agaccttctc
     1081 ctttgttgta cactataata tcatcacaaa ctcggtaagc ttcttcaata tcatgagtaa
     1141 caaatagtat atctcccttg taatctttta acatattcat aagttctttt tccatattgc
     1201 ttcttaaatg atggtccaat gctgagaaag gttcatctaa aagtaatata tctggagatg
     1261 ttgccaatgc tctagctagt gctacccttt gttgttgacc accagataat tgccaagggt
     1321 atctcccctc aaacccattt aatttaagta tatctaagta ccttgctact atttcctttt
     1381 tttcactctt acttagtttt aaaagcccaa gttctatatt ttgacttaca gtcatatgtg
     1441 gaaacaaagc atagttttga aataaaaatc ctacctttct atcttgtgtt gataaattta
     1501 tcttcttttc agaatcaaat agaactctat catttaatac aattttacct cttgttggtt
     1561 tctcaagccc tgctatacat tttaatgtca tactttttcc agaaccagat tctcccaaaa
     1621 accccagagt acctttttct tgtttaattt ctacctttag tttaaaagag gataaatctt
     1681 tttcaatatc cacgtataag ctcaactatc cacacctctt tcctataatt ttctgctgaa
     1741 attccgacca ataatttagt aataaaatca ttgctataga tattgctaca ataatcataa
     1801 cccacatcat agctttattc atatctccgc cttcaacagc aaaaaatata gctattggca
     1861 tagtttgagt ttttccaggt atatttccag ctatcataag tgtagcacca aattctccaa
     1921 gagctctggc aaacgacagc accaatccac caattattcc aggccatgct aatggaactg
     1981 caatcttaaa aaatactttc cattcattaa gacctaatgt tctagcagct gacaacatat
     2041 tattatcaat ctgttcaaaa gctgaccttg aagttctata catcatagga aaagacacca
     2101 ctactgctga aattacagtt gctgtccaac taaatattaa agtcctatcg aaatttaata
     2161 aaaactttcc tataaatcca ttcttcccac atagtaataa taaaaagaaa cctacaactg
     2221 ttggtggcaa aattagtgga agtgtaaata gtccatctat aagacccttc cacttacctc
     2281 tatagttagc cataatatat gacacaaata ttcctattac aaaagttatt attgttgaca
     2341 aaatagatgt cttaagtgat atccataagg gtgaccaatc tgttcccatc tttccctcct
     2401 taattttaaa tggaaatagt gaaactaata actctattaa tttcactatt tccattttat
     2461 tataatacac aattattttc aattttcatt tatttatata taatttatac ttaattacac
     2521 ataatttatt tataagttta ttctactttt ttatatccaa attcttcaaa aatcttttgt
     2581 cctgactcac ttaataaaaa ttcttcaaat ttcttagcag catctacgtt tttactagct
     2641 ttaattactg aaactggata tgtaataggt gaatgagttt tttcgtctgt tttttcaact
     2701 acttttatct tatcattatt gacagtatca ctgaagtaaa cgaatccaac atcagcattt
     2761 cctgattgca cccatgctaa aacttctttt acatcttttg caaatactag cttatctttt
     2821 aacttatctt ttaaatttaa attagttaac acctcatctg catacttacc agctggaaca
     2881 ctttcagctt caccaacagc aattttcttc actttatctg tagtcaaatc tttcatacct
     2941 gatacactac tgtcagcaga agaaataaga actaaatcat tttttactaa atctttcata
     3001 gtatcagaaa ctaataactt ctcttcatcc aaagctttca tttgtttttg acctgctgaa
     3061 ataaataaat cacaaggtgc tccttgttct atttgttgtt gtaatgagcc tgatgctcca
     3121 tagttaactg taagttttac atttgaatca acttttttat attcttcttc aatctttgcc
     3181 attgcttctt ttaaacttgc agcagccgat atatttaact ctactgaatc actactttta
     3241 gttgtttttt cttgttttcc atcatctttt ttgtcttcat tactattgca cccaactgtc
     3301 cccaaagtta aacatgcaac taatcctaat attcccaata tttttttcat aacaatctcc
     3361 ctttttgtaa atttgataca cattttaaca tgttttatta tgttttatca tgttttgtta
     3421 tatatagttt aattatatat acatgataat aaattttcaa gtaatttatt tacactactt
     3481 ttaaatattt tgctacattt tccttttttt aatgttaaaa taaccttata tggaggtgga
     3541 ttatggactc attatcatct ttgacacctg tcgaggtagc tgaattatta aagataacaa
     3601 aaaatactgt atatgaatta atcaaaagag gtgaacttcc ttcttataaa gtagggaaaa
     3661 aaataagagt tgatactaaa gatgttgaag aatatatcaa cagccaaaaa actggtaaag
     3721 taaaggatac acatttcaac aataaaaata tagatataaa taataatttc aaaattatta
     3781 tttctggtca agatgtaata ttagacattt tagctaggtc tgttgaaaaa aaattggatg
     3841 gaataagtac atttcgctct tatataggta gttataatgc tttgtatgag ttatataatg
     3901 gcagagtttc tatagcatca tcccatctat gggatttcga aacggatgaa tataatagca
     3961 cttttgttaa aaagctattg ccaggaatac catgtgtact tataaatcta gcttatagaa
     4021 tgcaaggttt ctatgttgct aaaggaaatc ctaaagagat aacaacctgg gaggatttaa
     4081 caaaaccaag tataaccatt gtaaataggg aaaaaggttc tggaactagg gttttactag
     4141 atggtaaact tagacttcta aactttaatg gtaaacatat aaaaggttat gataatgaag
     4201 aattatcaca tcttggagtt gcaagtactg tttctcgtgg aatagcagat gttggtttgg
     4261 gaaatgaaaa agctgcttta caagtaaata atatcgactt tattcctctt cacaaagagc
     4321 gctatgattt agttattaaa aaagaagact tacaaaatcc agtttaccaa actataatta
     4381 acataataaa ctctcctgag tttaaagctg aacttcaagg tcttggcgga tatgatttaa
     4441 ctgatactgg aaaaactata gcaaaagttt aagattaaaa tcgcaatcta ttttataaga
     4501 tttataattt ttcacaaaat ttataactta aacacgaaat aaattgcgat tttagtgtct
     4561 caaaaaattt atgtctttca ttttatcatc caatacaaaa tataatataa ttaattgtgc
     4621 aatcatatta tattaatata tatttacata atagtatttt tctttaatgt agattaacat
     4681 atgatatgtt ttaaacatca atccccttgc tttcttatat atatgtggca acacatatga
     4741 gtttaataaa ttatatacaa tgttgctttc ttcttgtatt ttttcaacca acaagttatt
     4801 tataaaatta cctttctcat tttcaataat ctcactccaa tttatcttaa cttttaatat
     4861 atatcttccc actaggtata cacactctac aataaataat aaaaatacaa tgcagtcagt
     4921 acctgttgtc ttatataaat tacccgatac taatggcttt gcgaagaaat tataaaaatt
     4981 caaagtatca cccgtataat caaaaagtat taatagcact cctagaactc tcaatacttt
     5041 aatattcaac atgtttttat ctttattatt tttgtttttc atatatacta cctctctttt
     5101 ctttagattt gattgtaatc taatactaac tcttttattt gaggtataaa tgaatttaat
     5161 tggtagttat tttcattttt actagaaatt aattcacatt actaaacaaa acttatattt
     5221 aaacataaaa atagcctatc tatcttttaa taaatacaaa aataaataag ctactaatat
     5281 tcaattaact tttatcactt aaaacattac tatctataaa tttaaatatt ttattgaagt
     5341 atactgtact atcatgttca aatccatcaa catgaccaat accttctata agccataggt
     5401 cttttttatc atgtttaact aaatcataca ttttcttgct attttgataa ttacaaacag
     5461 tatcttttgt tccatgtata aacagtatgg gtacattaga tttctcaact gactcatatg
     5521 gttttacatc actgtaccaa aatccagatt taaacttatt atataaatct ccacaagtca
     5581 ccacataatt gattaatata tctggtattc gtttttctag tactcccatt ctaattgcat
     5641 ctttcatttc agaataagga gaatccagta tataaaaatc gaccttatca tcttttgaat
     5701 ttatctctga atgcatagca gctgtccctg cccccatgga aaatccatgc actcctaatc
     5761 tcccttcagg aaatttattt tttacaaatt taactagact atctaaatca tacctttcat
     5821 aaagaccaaa tgtgtaatta tctccaccac tattaccagt atttctttga ttataaacta
     5881 atacattata acctttatct aaatatctat atgctacctt taacatttca tagtaactac
     5941 ttccaattcc atgaacaagt atcatagtat ctcttgtctt tatatttgaa gttataaaaa
     6001 gactttcaag cttgtaatta ttagaacttt gtatcattaa ttcagatgtc ttgtagtttt
     6061 ttaatttctc cagcggtcta tcttttctct cagaaaatat ttttactata tcttcgtttt
     6121 ttattaactg ctctaaacca actgaactat tatacactaa tttgccaaat atataagata
     6181 caattaatag gattaataga aacacaattg atataattat aaatttattt cttaaactaa
     6241 tacaattttt aaaactattt ttcatattta tttaccatac aattttccta tattatattt
     6301 tctaagtaat agcataattg attatactat tcttataatt tctattatac ctacttattt
     6361 attaaaaata caaatta
//
LOCUS       DBGJFF010000178         6307 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.182, whole genome shotgun sequence.
ACCESSION   DBGJFF010000178 DBGJFF010000000
VERSION     DBGJFF010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6307
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.182"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            124..1488
                     /locus_tag="ACOIAF_003549"
     CDS             124..1488
                     /locus_tag="ACOIAF_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HGZ2670529.1"
                     /translation="MDIKELVKMQRKYYNTGKTRDISFRIEQLKKLKSVVSQNEEKIL
                     LALKKDLNKSDFEGFMTEVGMFYSELNFAIKNVRKWSKIKRVKSSMVNFPSISKVVPQ
                     PYGVTLIMSPWNYPFQLALIPLVWSLAAGNCVILKPSEYSVSTSSVVKDIVENTFSKE
                     YVAVVQGSQEESEKLLLERFDYIFFTGSTNVGKIIMKSASEHLTPITLELGGKSPCII
                     LRDANIDLTAKRLTWGKLINAGQTCVAPDYVLVHEDRKNELIEKIKYYTNKYFGDNPC
                     NNEQFPKIINQRHFNRILSLIDKDKIVYGGNYNKETLKIEPTIVDNVNWDDNIMKEEI
                     FGPIFPILTYEDLDEVIQKIIQMPNPLALYIFTKNKYLENKLLKMIPSGGCCINDTIT
                     HIATNYLPFGGIGESGMGSYHGKAGFDTFTHYKSILKKLNLDVPIRYAPYDNKLIKIL
                     KKIM"
     gene            1626..2792
                     /locus_tag="ACOIAF_003550"
     CDS             1626..2792
                     /locus_tag="ACOIAF_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HGZ2670530.1"
                     /translation="MKINWNTKYTTIAIYTFIIAASSIIFYLVSSQIDVFSNNLDAIF
                     TTLQPFIIGFAIAYLLNFILKFYEDRIFIKSEKLKKLKQSSKRGLGLLLTYATATLIL
                     YLFMHFVLPQVIESIVGLANDIPMYVNNATKLIDKLMTDLNLDEQYFNLAVDKWNEFV
                     TYIIKFVTDLIPILGSMLKDVASSIWNIVLGLIVSVYLLIDKEKFYGLSKKITYAVFT
                     EKQAARILELTHRSNYTFGRFLGGKILDSFIIGILTFVILTLVKMPYTLLISVIIGIT
                     NIIPFFGPLFGAIPSTLIVMFVSPIKAFWLLLIILIIQQIDGNIIGPKILGDSIGISA
                     FWILFSLLVAGKLLGFIGMVIGVPMFAVIYSIIKDIVESKLDKKGLPTDTSDYM"
     gene            2909..3691
                     /locus_tag="ACOIAF_003551"
     CDS             2909..3691
                     /locus_tag="ACOIAF_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGZ2670531.1"
                     /translation="MAFIECKFKSKYTGGETDIIVILPVANGKELFSGKEIYDFEGKF
                     KTLYLFHGLEDDQSSWIRYTNVEHYAKNKNIALVIPRVETSFYTAMAYGHQYFTFVSE
                     ELPKFVRAIFPLSDKREDNFVAGMSMGGYGALKLALSKPKEFSAVASFSGSPDMIHEL
                     ENQQIETNADILFHDFGRPEDARNSENDLLYILKNLKERNEDIPKIYQFCGDRDFLYK
                     NNQMFKKYAESLGVDVEYEECVGGHEWRLWNLWIKKFIDIIG"
     gene            complement(3810..4463)
                     /locus_tag="ACOIAF_003552"
     CDS             complement(3810..4463)
                     /locus_tag="ACOIAF_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HGZ2670532.1"
                     /translation="MSKSEKGKETKASIISAARELFFEQGYHKTTTRQIAERANINLG
                     LISYYFSSKSEIGAIIYEDIRNEMQSLIYNYHEEGTMMMFFLAMNADLCFLLKNEAYR
                     NFYLEIAYEEAILSYQRSVTEMVMKRYVNDKESIEHDELILSCIGILAMKPEIIRVYT
                     SHKYDISSQAVIKHFTKQFLINLGHSTDLCEEILEELSKYYIDIVDNFTPIITRVRT"
     gene            complement(4487..5851)
                     /locus_tag="ACOIAF_003553"
     CDS             complement(4487..5851)
                     /locus_tag="ACOIAF_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HGZ2670533.1"
                     /translation="MLLLIVTCAIYIWIGFYFSKKVKTASDFLIAGRNLPLPILAATI
                     AATSFGGGCLVGGVQWGAERGLWVGMYSTIGAALACFINAFFAGKLRSLSCDITPADY
                     IETRYGHSVFLRAYQSLVTPISILAIMGSQLISFGSVSTAFGIPYDIAVIIGVIVIII
                     YTYTSGMWGVAVSAFIQLAICIVFLPIVAYISLKLVGDNPLLMLQSMVKEPFFPGNKS
                     ISDFMYTVLPLVLGSIFSYESFLRYQCADNAKNAVKSSVIAGFILLFLAFPIGIIGAI
                     GGSLFPNISSVQVLPQMISTTLPPLISVLFLAAILAAIMSTADSLMTSMSAIISRDIY
                     NKLLHPNIEFNDLKNTLKYSQIASALTAIIGAIIALKFDSLLELLFWPAPLSTGVIFA
                     PFVIGLCWKGATKKGAIYAVIVSVILALLNMIGILTVFDRILVPIIGGSITIFIVSKF
                     SKKD"
BASE COUNT         2409 a          784 c          936 g         2178 t
ORIGIN      
        1 ttattggaag atttatgtta tattgagatg aatttattat gatataaaaa ataatgttag
       61 gataaattta aatctttttc taaaaatgta ttagtataat tcttaaagaa aggattttta
      121 acaatggata taaaagagtt agttaaaatg caaagaaaat attataatac aggtaaaaca
      181 agagatatat cgtttcgtat agaacagttg aagaaactta agtcagttgt atcacaaaat
      241 gaagaaaaaa tacttttagc actaaagaaa gatttaaata aatcggattt tgaaggtttt
      301 atgacagagg taggcatgtt ttatagtgaa ttaaactttg ctataaagaa tgtaagaaaa
      361 tggagcaaaa taaaaagagt taaatcaagt atggttaatt ttccatctat atccaaagtt
      421 gttccacaac cttatggtgt tactctcata atgtcacctt ggaattatcc atttcaatta
      481 gcacttatac cattagtttg gtcattagca gcagggaatt gtgttatatt aaaaccttct
      541 gaatattctg tgtcaacatc tagtgtagtt aaagacatag ttgaaaatac tttttcaaaa
      601 gaatatgttg cagttgttca aggaagtcaa gaagaaagtg aaaagttact attggaaaga
      661 tttgattata tattttttac aggaagtact aatgttggta aaataatcat gaaatcagca
      721 tcagagcatt taactcctat tactttagaa cttggaggaa aaagtccttg tatcatttta
      781 agagatgcta atatagacct tactgccaaa agacttactt ggggaaaact aataaatgca
      841 ggacaaacct gcgtggctcc agattatgtt ttagtgcatg aagatagaaa aaatgagtta
      901 atagagaaaa taaagtatta tactaataag tattttggag ataatccatg taataatgag
      961 caatttccta aaatcataaa tcagagacat tttaatcgga tattatctct tattgataaa
     1021 gataagatag tctatggtgg aaattataat aaggagacac taaagataga gccaactata
     1081 gtagataatg ttaattggga tgataatata atgaaggaag aaatatttgg acctatattt
     1141 cccattttaa catatgaaga tttggatgaa gtaatacaaa aaattataca aatgccaaat
     1201 ccattagcac tttatatttt tacaaaaaat aagtacctag aaaataaact tttaaaaatg
     1261 attccatcag gtggatgttg tataaatgat acaattactc atatagctac gaattacttg
     1321 ccttttggtg gtattggtga aagtggtatg ggttcatatc atggaaaagc tggattcgat
     1381 acatttacgc actataagag catattaaaa aaattaaatt tggatgtccc tattagatat
     1441 gctccatatg ataataaatt aattaaaatt cttaaaaaga taatgtagtt gtattatgga
     1501 aaaataaata taaataaaat tgagcatttg tattctgaat atattttatt tttaatttgg
     1561 tatatttgtt ataaaagtgg tgattttgtg ataaaattta attatataga aataaggagg
     1621 aaaaaatgaa gattaattgg aatacaaaat acacaacaat agcaatttat acatttataa
     1681 tagctgctag tagtattata ttttatctag tatcatcaca aattgatgta ttttctaata
     1741 atttagatgc tatatttaca acattacaac cttttataat aggatttgct attgcatatc
     1801 ttcttaattt tattttaaag ttttatgaag acagaatttt tataaaaagt gagaaattaa
     1861 aaaaactaaa acaaagttca aaaagaggct tgggtctttt attaacatat gcaacagcta
     1921 cattgatact ttacttattt atgcatttcg tattgccaca agtaattgag agtattgttg
     1981 gactagcaaa tgatatacct atgtatgtaa acaatgcaac taagcttatt gataaattaa
     2041 tgactgattt aaatcttgat gagcaatact ttaatttggc agttgataag tggaatgagt
     2101 ttgttacata tattataaag tttgtaacag atttaatccc aatattagga agtatgttaa
     2161 aggatgtagc ctctagtata tggaatattg tactaggatt aattgtttca gtatacttat
     2221 taattgataa agaaaaattc tatggattaa gcaaaaaaat aacttatgca gtatttacag
     2281 aaaaacaagc tgccagaatt ttagaattaa ctcatagaag taactataca tttggaagat
     2341 ttttaggtgg caaaatatta gattcattca ttataggtat tctaacattt gtaatactta
     2401 cattagttaa aatgccatat acattattaa tatcagttat aattggtatt acaaatatta
     2461 tccctttctt tggaccttta tttggagcta taccatctac gctaatagtg atgtttgtat
     2521 ctcctataaa agctttctgg ttattgttga ttatattaat aatacaacag atagatggaa
     2581 atattatagg acctaaaata cttggagatt caattggtat atctgcattt tggattttgt
     2641 tctccttatt ggttgcagga aaactattag gctttattgg aatggttata ggagttccaa
     2701 tgtttgcagt aatttattct ataattaagg atattgttga aagtaaattg gacaaaaagg
     2761 gattaccgac agatacttcc gattatatgt agctaaaaaa tattatgtat aattaaaaat
     2821 atataaatta agcaatattt aaagcactga gaaagttaaa ctcttggtgc tttttagata
     2881 taaaattatt atagaagagg tgttttttgt ggcatttata gaatgtaaat ttaaatctaa
     2941 atatactgga ggagaaactg atataattgt aattttgcct gtagcaaatg gaaaagaact
     3001 ttttagtggt aaagagatat atgattttga aggtaaattt aaaacattat acttatttca
     3061 tggtttagaa gatgaccaaa gctcatggat tagatacact aatgtagaac attatgctaa
     3121 aaataaaaat atagccctag taataccaag agtagaaact agtttttata cagctatggc
     3181 gtatggacac caatatttta cttttgtaag cgaggaatta cctaaatttg ttagagctat
     3241 atttccttta tcagataaaa gggaggataa ctttgtagct ggtatgtcta tggggggata
     3301 tggagcttta aaattagcac tttcaaagcc aaaagagttt tcggcagttg caagtttttc
     3361 tggttcgcct gatatgattc atgagttaga aaatcaacaa atagaaacca atgcagacat
     3421 tctatttcac gattttggta gaccagaaga cgctagaaat agtgagaatg atttacttta
     3481 catacttaag aaccttaaag aaagaaatga agatattcct aaaatttatc aattttgtgg
     3541 tgatagagat tttttatata aaaataatca gatgtttaaa aaatatgcag aatctctagg
     3601 tgtagatgtt gaatatgaag agtgtgtagg ggggcatgaa tggcgccttt ggaatttatg
     3661 gataaagaaa tttatagata taattgggta gtacacttat ggtattaaca gtgatactat
     3721 aaaatatctt taaatatgat aaagactgaa atcctaaaat caaattatga ttttaaaatt
     3781 tcagtctcta agaatttaat aaaattttat taagttctta cacgagttat tattggagta
     3841 aaattgtcaa ctatatctat atagtattta ctcaattcct ctaaaatctc ttcgcataaa
     3901 tccgtactat gaccaagatt aataagaaac tgttttgtaa aatgctttat tacagcttga
     3961 gaagatatat catacttgtg tgaagtataa actctaatta tttcaggttt catagcaagt
     4021 ataccaatac aagaaagtat taactcatca tgttctatag attctttatc attaacatat
     4081 cttttcatga ccatttcggt tacacttctt tgatagctta aaatagcttc ttcataagct
     4141 atttccaaat aaaaattcct atatgcttca ttttttagaa ggaaacaaag gtcagcattc
     4201 atagcaagaa agaacatcat catagtacct tcttcatggt agttataaat taaagactgc
     4261 atttcatttc ttatatcttc atatattata gctcctattt cgcttttact tgagaagtaa
     4321 tagcttatta gacctaaatt tatattagct ctttctgcaa tttgcctagt tgttgtctta
     4381 tgataacctt gttcaaaaaa taattctcta gcagcactta taatactagc ttttgtttct
     4441 ttgccctttt ctgattttga catatttact gtgtatacct ctcttttcaa tctttttttg
     4501 aaaatttgct aactataaat attgtaatag aaccacctat tataggaacc aaaattctgt
     4561 caaatacagt aagaatacca atcatattta ataatgccaa tataacactt actataacag
     4621 catatatagc cccttttttt gtagcacctt tccagcaaag accgattaca aatggtgcaa
     4681 atattacacc tgtacttaat ggagctggcc aaaataataa ttctaataaa ctatcaaatt
     4741 taagcgctat aattgcacct attatagctg tcaaagctga tgcaatttga gaatacttta
     4801 gagtattttt taaatcatta aattctatat taggatgtaa taatttgtta taaatatctc
     4861 ttgatataat agcactcatt gaggtcatta atgaatcggc agtagacatt atggcagcca
     4921 atatagcagc taagaataat acagatatta aaggtggcaa agtagtcgat atcatttgag
     4981 gtaaaacctg aacagatgat atatttggaa ataaactacc accaatagct ccaattattc
     5041 ctatagggaa agcaagaaat aatagtataa atcctgcaat tacactactt ttaactgcat
     5101 ttttagcatt atcagcacat tgatatctta aaaaactttc ataagaaaat attgagccta
     5161 aaactaatgg taaaactgta tacataaaat cactaatcga tttatttcct ggaaagaatg
     5221 gttcttttac cattgattgt aacataagta aagggttgtc gccaactaat tttaagctta
     5281 tataagctac tattggcaaa aacactatac aaatagcaag ttgaataaat gctgatactg
     5341 caactcccca cattcctgat gtataagtat atataatgat aactataact cctataatta
     5401 cagctatatc ataaggaata ccaaatgcag tagatacaga accaaaagaa attaattgac
     5461 tacccataat agctagtata gatattggtg ttacaagact ttgataagct cttaaaaata
     5521 cactatgacc atatctagtt tcaatataat cagcaggtgt tatatcacaa gataagcttc
     5581 ttaattttcc tgcgaaaaat gcatttataa agcaagctaa agctgcacct atagtagaat
     5641 acattccaac ccaaagtcct ctttcagctc cccattgaac tccaccaact aagcaccctc
     5701 ctccaaaaga tgtagcagca atggttgcag ctagtatagg caaaggcaga tttctacctg
     5761 cgattaggaa atcggatgct gttttaactt tttttgaaaa ataaaaacca atccatatgt
     5821 atattgcaca cgtaacaatt aaaagtaaca tagtcttttc tcctttataa aatattttaa
     5881 taaaatatac aaaatatatt taaaatatat tttatataaa agtagtacga tatttgtttt
     5941 attggtttta gattaatata gctataataa tttatgatat tagatggaaa ttacgtaaaa
     6001 atagaaaatt gaaaattctg aaatttttgc atattgatat ttatctcata gattcttttt
     6061 tgtaattgta acacgctttt atttttaaat caatagtaca tgtataaaaa taaaaggaat
     6121 ttaaataaga aaaagatatt tttaatatct ggatgtagta tagaaatatc ttgaataaat
     6181 tattcttgaa taaaattata aaaaattaaa atcactagaa atatgataaa ataatttaaa
     6241 atacgcattt caatgacatt tcaaaacaag aaaataataa aaaacaaacc taaattaagt
     6301 ctaaata
//
LOCUS       DBGJFF010000179         6141 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.183, whole genome shotgun sequence.
ACCESSION   DBGJFF010000179 DBGJFF010000000
VERSION     DBGJFF010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6141
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.183"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            320..1060
                     /locus_tag="ACOIAF_003554"
     CDS             320..1060
                     /locus_tag="ACOIAF_003554"
                     /inference="COORDINATES: protein motif:HMM:NF024744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HGZ2670534.1"
                     /translation="MKNFRDLGGNKTEDGRTVKKGLFYRSAKLSNLSENDIKILKELN
                     IKYIFDYRSDEEARKHPSTIISNIKNIRIPAMRELEESGGSFGSIEDMIDGLFEKDGA
                     FNMLNNSYYNLPINNPSYKKLVELIRDYSNLPILNHCTAGKDRTGVGSAIILMILGVS
                     RENIMKDYLKSNDFADKEIERFIEYKPKFKDIPKENLKYIFGVNEEYMKTAFRRIDEE
                     YISVEAYLYGEFNLNKEEIRKLRNQYLE"
     gene            1072..2850
                     /locus_tag="ACOIAF_003555"
     CDS             1072..2850
                     /locus_tag="ACOIAF_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HGZ2670535.1"
                     /translation="MSLNITARQINIIKILLNSKDTISGIALSQEIGCSSKTIQNEIK
                     DINKQFRNGKILSIRGVGYKLEGSFDEVNINSNLYDDVDRVEYIIRKILTLSNEEKNT
                     IKLEDLADSMYVSVSTVKNDLKEVKRMLEKYSISVKSKHKQGICVLESEDKILNCIVD
                     LSNKRDNQLSLNDFLNDDIKERKLLIKKLLLDILNENGLVLTDIEFKSVLNNILVMLS
                     RHKYNEKEFIENYIENYRSKRSSILENEQNKEIIINSIKTFCKNLKLATSIDISNDKA
                     FEEFLYKHISSLCKKIKLGINQSPIMAQDIKIKYPFAFELANIAKKTIEKDLKIEINE
                     DEVANIALHIGGAVERASYNNKDKVFKTIIVCTSGIGTSMLIKAKLENIFKEKLEILK
                     IIPSYLVEYIKVIDVDFVISTVPIEVGDVPVINISPILSEKEIRLIEKYIETGNIYLD
                     LDIKSLFDSELFFTDLVFKRKEEVIDFMASKLVQKGYIDEDMKKSYFEREKIATTEIG
                     NMVTIPHGAIGKIFDNKIAIGILKKPISWEISDVRLIIMLALDKDKILDYEVIFSKIY
                     KRVDSIAKVISICENSSFEKFVNLFN"
     gene            3017..3934
                     /gene="pfkB"
                     /locus_tag="ACOIAF_003556"
     CDS             3017..3934
                     /gene="pfkB"
                     /locus_tag="ACOIAF_003556"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HGZ2670536.1"
                     /translation="MIYTVTLNPSIDYIVKLDELKTGSTNRVKEEYVYPGGKGINVSR
                     ILKELGNDNTSLGFISGFTGEYIIRTLEEKELKTDFIKIKSGFSRINVKIKALKETEI
                     NGQGPNIDDEDIDTLYKKLDKLNQDDILILAGSIPSTLDEKLYENIMARLEKKNIKVV
                     VDATKNLLLNVLKYKPFLIKPNNDELEELFGVKLNSIEDMVKYARRLKEMGAINVLVS
                     MGKDGALLITEEDEVLISDVPKGKVKNSVGAGDSMVAGFISGYLNTGKYDYALKLGAA
                     SGSATAFSYDLAKREYIDKLVNEISVKQF"
     gene            3971..5890
                     /locus_tag="ACOIAF_003557"
     CDS             3971..5890
                     /locus_tag="ACOIAF_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HGZ2670537.1"
                     /translation="MKIIDLLDEKSIKLNLNSKTKSEAIEELVDLVANSGNLNDKENY
                     KKAILAREEMSTTGIGEGVAIPHAKNSSVTKACIAAAVSKEGIDYESFDGSLSNLFFM
                     IAAPDGANNTHLEVLSRLSTILMDEDFRNKLINSSSEKEFKEIIDKKEREKFSEEYQD
                     EKVTEKNIIDTKENDANKYPKVLAVTACPTGIAHTFMAAESLNKMAENKGVSIKVETN
                     GSAGVKNKLTKDEIENATCIIVAADKNVEMARFNGKKVIKTKVADGIHKAEELIDKAV
                     NGDAPIYHGGDGSHNESNEESESGFRKVYKHLMNGVSNMLPFVIGGGILIAIAFLLDD
                     YTINPSNFGSNTPIAAFFKGIGDKAFGFMLPVLAGYIAYSISDRPAFVVGFVGGALAG
                     DGGSGFLGALLAGFIAGYLVEGLKKIFSVLPASLEGIKPVLLYPLLGTLLMGIIMTFL
                     VIPPVAAINNAMVGFLNGLGGTSKIVLGLVLGGMMAVDMGGPVNKAAYVFGVASLESG
                     QFEIMAAVMAGGMVPPLAIALATTFFKNRFTKEERDSGKVNYVMGLSFVTEGAIPFAA
                     GDPLHVIPACVGGSAVAGALSMLFNAALRAPHGGVFVIPVVTHPFAYILAIAVGALVG
                     MMFLALLKKPLNQEV"
BASE COUNT         2531 a          559 c         1106 g         1945 t
ORIGIN      
        1 catatgtaaa tttaattata aaattctttt gcttttgaat atccataata taaaacctcc
       61 aatgtatttg atattattct aaatatggtc ttttttacag aaaatatact actgtatatt
      121 gattataaac tataaagctt atgtaaagta aataaattat gtaaaattta ggttaatttt
      181 gtataaagag atacttttat attttacaat taataaccaa atactaaaaa atattttaaa
      241 taaagaactt aaaattgtga taaaattaat ttatagatat taaaaaaagt ataatatatg
      301 gtttttaaga ggtgctaaga tgaaaaattt tagagattta ggtgggaaca aaactgagga
      361 tggcagaact gtaaagaagg gattatttta taggtctgca aaattatcaa atctaagtga
      421 aaatgatata aagattttaa aagagttaaa tattaaatat atatttgatt atagaagtga
      481 tgaagaagct cgtaaacatc catcaacaat aatatcaaat ataaaaaata taagaatacc
      541 agcaatgaga gagctagaag aatcaggagg aagttttggt tctattgaag atatgattga
      601 tggattgttt gaaaaagatg gtgcatttaa tatgttaaat aatagttact ataatttgcc
      661 tataaataat ccgtcatata aaaaattagt agaacttata agagactatt ctaatttacc
      721 aatattaaat cattgtacag ctggtaagga tagaactggt gtggggagtg ctataattct
      781 tatgatttta ggggtatcga gagagaatat aatgaaagat tatctaaaaa gtaatgattt
      841 tgcagataaa gagatagaaa gatttataga atataagcct aaatttaagg acattccaaa
      901 agaaaattta aaatatatat ttggtgtaaa tgaagagtat atgaaaactg catttagaag
      961 aattgatgaa gaatacatta gtgtagaagc atatttatat ggagaattta atttaaataa
     1021 agaagaaata agaaaattaa gaaatcaata tctagagtaa aaggtgagtt tatgagttta
     1081 aatataacag caagacagat aaatataata aaaatattgt taaattcaaa agatacaatt
     1141 agtggtattg ccttaagtca agagatagga tgttcttcaa aaactataca aaatgaaata
     1201 aaggatatta ataaacaatt tagaaatggt aaaattttgt caataagagg agttggttat
     1261 aaactagaag gaagttttga tgaggtaaat ataaatagca atctctatga tgatgtagat
     1321 agagtagaat atataatcag aaaaatatta accttaagta atgaagaaaa aaatactata
     1381 aaacttgaag atttagccga ttctatgtat gtaagtgttt caacagtaaa aaatgattta
     1441 aaagaagtaa aaagaatgtt ggaaaagtat tcaatatcag ttaaatctaa gcataaacaa
     1501 ggtatatgtg ttttggaaag tgaagataag atattaaatt gtatagttga cttaagcaat
     1561 aagagagata atcaattgag tttaaatgat tttttaaatg atgatataaa ggaaagaaaa
     1621 ctattaataa aaaaattatt attagacata ttaaatgaaa atggattggt attaactgat
     1681 atagaattta agagtgtgtt aaataatata ttagttatgt taagcagaca taagtataat
     1741 gaaaaagaat ttatagagaa ttatatagaa aattatagga gcaaaagaag ctcaatttta
     1801 gaaaatgaac aaaacaaaga aattataata aattcaataa aaacattttg taaaaattta
     1861 aaattagcta catcaataga tataagtaat gataaggcat ttgaagagtt tttatataag
     1921 catattagta gtttatgtaa aaaaataaag ttaggtataa atcaatcacc tataatggct
     1981 caagatataa aaataaaata tccatttgct tttgaactag ctaatatagc taaaaagaca
     2041 atagaaaaag atttgaagat agaaattaat gaagatgaag ttgcaaacat agctctacat
     2101 ataggaggag cagttgaaag agcatcttat aataataaag ataaggtttt caaaactata
     2161 atagtttgta cttcaggcat aggcacatca atgttaataa aagctaaatt agagaatata
     2221 tttaaggaaa aattagaaat actaaagatt attccttcat acttagttga atatataaaa
     2281 gttatagatg ttgattttgt aatatctact gttcctattg aagtaggaga tgtgccagta
     2341 ataaatatat ctccaatttt aagtgagaaa gaaattagat taattgaaaa atatatagaa
     2401 acaggaaata tatacctaga tttagatata aaaagcctgt ttgatagtga attatttttt
     2461 actgatttgg tgtttaaaag gaaagaagaa gttatagatt ttatggcaag taaacttgta
     2521 caaaagggtt atatagatga ggatatgaaa aaatcttact ttgagagaga aaaaatagca
     2581 acaacagaaa tagggaatat ggtaactata ccacatggag ctattggaaa aatttttgat
     2641 aataaaatag caattggtat attaaagaag cctatttctt gggaaatatc agatgttcgt
     2701 ttaataataa tgttagccct tgataaagat aaaatattag attatgaagt aatattttca
     2761 aaaatatata aaagagtaga ttctatagca aaagttataa gtatatgtga aaatagtagt
     2821 tttgaaaaat ttgtaaattt atttaattag atgggtagta ttgtatatac acctgtaaat
     2881 ttggataata taaaactaaa taagataaaa acgataaatt tctcttttaa ttaagagaaa
     2941 ttttttgact ttcaaggata ttaatataaa gttaagatat aaacataaag caaataaata
     3001 aaaaagaggt ggcaatatga tttatacagt gacattaaat ccatcaatag attatatagt
     3061 taagctagat gagctaaaga caggaagtac aaatcgtgta aaagaagaat acgtttatcc
     3121 aggaggaaaa ggtataaatg tatctcgcat acttaaggaa cttggaaatg ataatacatc
     3181 tttaggtttt ataagtggtt ttactggaga atatataata agaacattgg aagaaaaaga
     3241 attgaagaca gattttataa agataaaaag tggtttttca agaataaatg taaaaataaa
     3301 ggcgttaaag gaaactgaga ttaatggaca aggtccaaat atagatgatg aagacattga
     3361 tactttatat aaaaaacttg ataagctaaa tcaagatgat attttaatat tggcaggaag
     3421 tataccatct actttagatg aaaaactata cgaaaatata atggctagat tagaaaaaaa
     3481 gaatataaaa gttgtagtag atgcaactaa aaatcttctt cttaatgttt taaaatataa
     3541 accttttcta attaagccta ataacgacga attagaagaa ctatttggtg ttaaattaaa
     3601 ttcaattgaa gacatggtaa aatatgctag aaggttaaag gaaatgggag ctataaatgt
     3661 cttggtatca atgggaaaag atggagcatt gttgattaca gaggaagatg aagtgcttat
     3721 aagtgatgtt ccaaaaggaa aagtaaaaaa ttctgtagga gcaggtgatt caatggtagc
     3781 aggatttata tctgggtatt tgaatactgg gaaatatgat tatgctctta aattgggagc
     3841 agcttcaggt agtgcaactg ctttttcata tgacttagcc aaaagagaat acattgataa
     3901 attggtaaat gagatatctg taaagcagtt ttaaaatatt aaaaaatata tagaaaggta
     3961 attaatattt atgaaaatta tagatttatt agatgaaaag tcaattaagt taaatttaaa
     4021 ttcaaaaacc aaatcagaag caatagaaga attagtagat ttggtcgcaa atagtggaaa
     4081 tttaaatgat aaagaaaact acaaaaaagc aattttggct cgtgaagaaa tgagtacaac
     4141 aggaattgga gaaggtgtag ctatacctca tgctaaaaat tcttcagtta ctaaagcttg
     4201 tatagcagca gcagtgtcaa aagaaggtat agattatgaa tcgtttgacg gaagtttatc
     4261 aaatctattt tttatgatag ctgcaccaga cggagctaac aatactcatc ttgaagtatt
     4321 atcaagatta tctacaatac ttatggatga agactttaga aataaactta taaattcaag
     4381 ctctgaaaaa gaatttaagg aaataataga taaaaaggaa agagaaaaat tcagtgaaga
     4441 atatcaagat gagaaagtta ctgaaaaaaa tataatagat actaaagaga atgatgctaa
     4501 taagtatcct aaggttttag cagtaacagc atgtccaact ggaatagcac atacatttat
     4561 ggcagctgaa agtttaaata aaatggcaga aaacaaaggt gtatcaataa aagtagaaac
     4621 taatggttca gcaggtgtta aaaataagct tacaaaagat gaaatagaaa atgcaacatg
     4681 tataatagtg gcagcagata aaaatgttga gatggctaga tttaatggca aaaaagtcat
     4741 aaaaactaag gtggctgatg gtatacacaa agcggaagaa cttatagaca aggctgtaaa
     4801 tggagatgct ccaatttacc atggaggaga tggaagtcac aacgaatcaa atgaagaaag
     4861 tgaaagtgga tttagaaaag tatacaaaca cttaatgaat ggtgtgtcta atatgttacc
     4921 atttgttatt ggtggaggga tacttatagc gatagccttt ttacttgatg attatactat
     4981 taacccttca aactttggtt caaatacacc tatagcagca ttttttaaag gaataggaga
     5041 taaagctttt ggatttatgt tacctgtact tgctggatat atagcatact ctatatctga
     5101 tagaccagcg tttgttgtag gatttgtagg aggtgctcta gcaggtgatg gtggttcagg
     5161 ctttttaggt gcactacttg caggttttat agcaggttat ttagtagaag ggcttaagaa
     5221 aatatttagt gtactaccag cttcattgga aggaataaaa ccagtattgt tatatccttt
     5281 acttggaaca cttttgatgg gaattataat gacattttta gtaatacctc cagttgcagc
     5341 aattaataat gcaatggttg gatttttaaa tggtttaggt ggaacttcaa aaatagtttt
     5401 aggattagta ttaggtggaa tgatggctgt ggatatgggt ggacctgtaa ataaagcagc
     5461 atatgtattt ggagttgcat cacttgaatc agggcagttt gaaataatgg cagcagtaat
     5521 ggctggtggt atggttccgc cacttgctat agctcttgca actacatttt ttaagaatag
     5581 atttactaaa gaagaaagag attctggtaa agttaattat gtaatgggat tatcatttgt
     5641 tactgaagga gcgatacctt ttgcagctgg agacccactt catgttatac cagcttgtgt
     5701 tggaggttca gcagtggcag gagcacttag tatgttgttc aacgcagctc ttcgtgcacc
     5761 tcatggagga gtatttgtta taccagtggt tacccatcca tttgcatata tattagctat
     5821 agctgtaggt gctttggttg gaatgatgtt tttagcatta cttaaaaaac cattaaatca
     5881 agaagtatag caattaaagg atattcaaga acttaaaata tgatgtttta taaaaattaa
     5941 tatattgctg aattatttta aaataaggat tttatgaggc tgttatataa cgatttgtaa
     6001 ttgttatctt gacagtcttt tatttttata taagtaaaat aagtgtgttt tgctttattt
     6061 gtatttaaat tgtttttaag ttagaatata aagaaaatta tatattattt taatattcac
     6121 tcgaactaga aaataatatt t
//
LOCUS       DBGJFF010000180         6139 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.184, whole genome shotgun sequence.
ACCESSION   DBGJFF010000180 DBGJFF010000000
VERSION     DBGJFF010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6139
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.184"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            276..1088
                     /gene="modA"
                     /locus_tag="ACOIAF_003558"
     CDS             276..1088
                     /gene="modA"
                     /locus_tag="ACOIAF_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HGZ2670538.1"
                     /translation="MFKKLSVLALAMIISLSITACNSKENKANDESNTKVTTADDSKE
                     KETLFVYSGAGLKKPMDEIAKKFEKENNVKVEYSYAGSAQLIAQIETSHKGDVFIVGS
                     EPIYSKAFEKELVGEHKTVAHHTPAIVVPKGNPANIQKLEDLNKDGVKLILGDKESNA
                     IGKTTQKILEKNNLKSINDNVVSTAATVNEMIVQLTSSKADATIATKDSVFGNEDVEV
                     IEIAQDKNIDQLISAGIVNYSDKKDLANKFINYIESDEAKQIFKKYGFEPVK"
     gene            1098..1889
                     /locus_tag="ACOIAF_003559"
     CDS             1098..1889
                     /locus_tag="ACOIAF_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2670539.1"
                     /translation="MKNKFFTTLLTGFFAIVFLFIVSPLLMLIIKGISYVPICLKSVE
                     VQYAITLSIKTSLISTIVCLLLAIPVAYFLHITKLPFKKLIIQIINLPMSLPHLVSGI
                     ALLLLFGRMGIGDSIYKIFKLDFIFTKQGIVLAQVFVNLPLTIKILHTSLNESNEKMI
                     FVARTLGCNSWEAFRFIILPNLKTGIISATVMTWSRALGEFGAVAMIAGSTRMKTEII
                     PTSIYLNMSTGDIDIAIGIAVILIFISLTCLMLFEIFFNREVDKN"
     gene            1891..2586
                     /locus_tag="ACOIAF_003560"
     CDS             1891..2586
                     /locus_tag="ACOIAF_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGZ2670540.1"
                     /translation="MLKIKNLSLDLPNFKLQENSLNINNHEYFVLLGASGSGKTLFLE
                     TLAGRYPSAKGNVSYKENLISNLPPENRNIGFVYQNFELFPNMNVEDNIRFPLKLRKI
                     PIDEQILKANHLSKLMQIEDIKNRYPKNLSGGEKQRVAFARALIAKPDILLLDEPMSS
                     LDYITKKHISKILKDVYIKYKPTVIHVTHDISEAMFFAHKIGIMNNGKINHIFDLNEE
                     IKNKGESFFYEYI"
     gene            2573..3304
                     /locus_tag="ACOIAF_003561"
     CDS             2573..3304
                     /locus_tag="ACOIAF_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF364 domain-containing protein"
                     /protein_id="HGZ2670541.1"
                     /translation="MNIFDILRDYLLVQVDKYNLNMDMISIVSKSLSSKEAIGNTKRK
                     DFPIIVGKEIMLEADFKGAKGQAFTSTPSTFEGSLKDILSLDLHDNPHDRSLFIASLN
                     AVMKYLGKTDRTIHCKNNEPEVCAKKFPEFIKMEFGNPKVAIIGYQPAIIDNIKDFFE
                     TRVLDLNPEFVDTIQYNVKIEDGIRDYEDVISWADLVICTGSTLCNNSIINFLSLNKP
                     VYYYGTTIAGASNILGLKRLCFCSK"
     gene            3618..4418
                     /locus_tag="ACOIAF_003562"
     CDS             3618..4418
                     /locus_tag="ACOIAF_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HGZ2670542.1"
                     /translation="MTFKNKKFTINKYKILSIILVLCLILTAFSSYVNYSHKKFKDYV
                     VFYVQHQDDEVLWAGSAIVNAIKERGKNHVFVVLVSTGCGISVFDKYKKYENLTNIQK
                     SEYRNREFLASLNSLGVIRENIILLPETNTNGNTDFNYMEKIALTFEEKFKNVTHIAH
                     TYKLDDHLQHLKNGSVIQNLYNAGKIKDAKYFVKPKFADKITFNNKIVYKAHSTEDYE
                     KVKNACQQYKIVDELEYREGIGYKSDHKSFDKLLNNKDVPAILHTPNL"
     gene            4958..5674
                     /locus_tag="ACOIAF_003563"
     CDS             4958..5674
                     /locus_tag="ACOIAF_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGZ2670543.1"
                     /translation="MNQNILVVDDDREIVDAIEFYLRPDNFNVLKAYDGLEALDILIE
                     NDIKLIIMDVMMPNLDGLKTTLKIREKRNIPIILLSAKSEDMDKIIGLNMGADDYITK
                     PFNPLELTARVKSQLRRYINLGDFSNTNNSNDTNYILKSGGLELNTDTKIVSLDGEEI
                     KMTPLEYKILTLLMSRKGKIFSSHEIYENVWNEDAYSCERTIAVHIRRIREKIEINPK
                     EPKYLKVVWGIGYKIEKLNY"
     gene            5643..>6139
                     /locus_tag="ACOIAF_003564"
     CDS             5643..>6139
                     /locus_tag="ACOIAF_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HGZ2670544.1"
                     /translation="MVTKLKNLIINDSKAKFILLLLLFLLVSILFEAVYDLNNNFSNI
                     NYYYREFALPYFYFFGTPVHLSKVELEFVCIGIILSSFLLTILCFTLYKKNKQTKVKF
                     LENVISLFSKVKIELTLILTLILLTLRFHAFTSNKIFYNTNLIIYNFIIISIVYIIYE
                     NIKYN"
BASE COUNT         2421 a          734 c          847 g         2137 t
ORIGIN      
        1 tgaaatataa taaatataaa attagttaaa aaataaaaaa tctacaataa gttaatttga
       61 tttaagtttt taaatttaaa atactttaaa gtattaataa aaattaaaaa taaaataact
      121 acctatggaa atgacattat aaatagattt tttcttaact atacataaca aaacataaca
      181 aaacattata aaacataaca aaaacttaga catcgttttc ttagtatgat atactaaaaa
      241 agaatttaca ctaatttact aagggggaat atcttatgtt taaaaaactt tcagtactag
      301 ctttagctat gattatttca ttaagtatta ctgcatgtaa ttctaaagaa aacaaagcta
      361 atgatgaatc taatactaaa gtgacaacag ctgatgattc aaaagaaaaa gagactcttt
      421 ttgtctattc aggggcaggt ttaaagaaac caatggatga aatagcaaaa aaatttgaga
      481 aagaaaataa tgtcaaagtt gaatatagct atgcaggttc tgcacagcta atagctcaaa
      541 ttgagacaag tcacaaagga gacgtattta ttgttggttc tgaaccaatt tattcaaagg
      601 cctttgaaaa agaacttgta ggtgaacata aaacggttgc acatcataca cctgcaatag
      661 ttgtacctaa aggaaatcca gctaatattc aaaaattgga agacttaaat aaagatggag
      721 ttaaacttat tttgggagac aaagagtcta atgctatagg taaaactaca caaaaaatac
      781 ttgaaaaaaa taatttaaaa tctataaatg ataatgttgt ctctacagct gcaactgtaa
      841 atgaaatgat tgtacagtta acatcaagta aagctgatgc tacaatagcc acaaaagatt
      901 cagtctttgg caatgaagat gtagaagtta tagaaattgc ccaagataaa aatatagacc
      961 aacttatatc agcaggaatc gtaaactata gtgataaaaa agatttagct aataaattta
     1021 taaattatat agagtcagac gaagctaaac agatatttaa aaaatatggt tttgaacctg
     1081 ttaagtagtg attaaacttg aaaaataaat tttttactac actacttact ggattttttg
     1141 ctatagtctt tttgtttatt gtatcaccac ttcttatgtt aatcattaaa gggataagtt
     1201 atgttcctat atgcttaaaa tcagtggaag tacagtatgc aataacactt agtattaaaa
     1261 catctttaat atctactata gtatgccttt tattagctat tccagtagca tattttttac
     1321 atattactaa attaccattt aaaaaactga ttatacagat aataaatctc cctatgtctc
     1381 ttccacactt agtatcagga attgctcttt tacttttgtt tggaagaatg gggattggag
     1441 attccattta taagattttt aagcttgatt ttatatttac aaagcaaggt atagttttag
     1501 cacaagtatt tgtaaatctt ccacttacta ttaaaatact tcacacttca ctaaatgaaa
     1561 gtaatgaaaa gatgatattt gttgcacgta cccttggatg taatagttgg gaagcattta
     1621 gatttataat tcttccaaat ctaaaaactg gaatcatatc tgcaactgta atgacttggt
     1681 caagagcact tggtgaattt ggtgcagtag ctatgatagc aggttctaca cgtatgaaaa
     1741 ctgaaattat tcctacatct atatatttaa atatgtctac tggtgatata gacattgcaa
     1801 tcggaatagc tgtaattttg atatttatct cacttacatg cttgatgctt tttgagatat
     1861 tttttaatag agaggttgat aagaattaat atgttaaaaa taaaaaattt atctcttgat
     1921 ttacctaatt ttaaattaca agaaaatagt ttaaatatta ataatcatga atactttgtt
     1981 ttattaggag ctagtgggtc aggtaaaact ctattcctag aaacattagc aggaagatat
     2041 ccatctgcta aaggaaatgt atcatacaaa gaaaatttaa tatcaaactt acctccagaa
     2101 aatagaaata taggatttgt atatcaaaat tttgaattat ttccaaatat gaatgttgaa
     2161 gataatataa ggtttccact taaattaaga aaaataccta tagatgaaca gattcttaag
     2221 gctaaccact tgtctaaatt aatgcagata gaagatataa aaaataggta tcctaaaaat
     2281 ttaagtggag gcgaaaagca acgagttgct tttgcacgtg ccttaatagc taaacctgat
     2341 atattacttt tagatgaacc tatgagttca ttggattata ttacaaaaaa acatataagc
     2401 aaaattttga aggatgtcta cataaaatac aaaccaacag tgattcatgt tactcatgat
     2461 atttctgaag caatgttttt tgctcataag ataggaataa tgaacaatgg aaaaataaat
     2521 catatttttg atttaaatga agaaataaaa aataaaggag aatctttttt ctatgaatat
     2581 atttgatatt ttgagagatt atctattagt acaagtcgat aaatataatt taaatatgga
     2641 tatgataagt attgtgtcca aatctctatc ttcaaaagaa gcaattggaa atactaagcg
     2701 taaagatttt ccaattatag ttggtaaaga aataatgctt gaagctgact ttaaaggagc
     2761 taaaggacaa gcatttactt ctactccatc tacatttgaa ggttctttaa aagacatatt
     2821 atcattagat ttacatgaca atcctcatga tagaagtctt tttatagcat cattaaatgc
     2881 agttatgaaa tacttaggca agacggatag aaccattcat tgtaaaaata acgagccaga
     2941 ggtatgtgca aaaaaattcc ctgagtttat aaaaatggaa tttggtaatc caaaagtagc
     3001 aattatagga tatcaaccag ccataattga taatattaag gactttttcg aaacaagagt
     3061 attagattta aatcctgaat ttgtggatac tattcaatat aatgttaaga ttgaagatgg
     3121 tatacgagat tatgaagatg taatatcttg ggctgattta gtgatttgca caggaagtac
     3181 attatgtaat aactcaatta taaattttct atcacttaac aaaccagttt actattatgg
     3241 aactacgata gctggtgcat caaatatttt aggtttaaaa agactctgtt tttgtagtaa
     3301 ataagcaatc atatcatcaa ggagtacaca aatatatatg tgtactcttt aaatttaaaa
     3361 agttattact tttattctaa gtactaaagt aaaattctat taaaatgaat cccgaataaa
     3421 tgtacaatcc ctttaaatct caattcctat caatcattaa tattttgatt agtcttatac
     3481 ataaatgttt tctttatttt aaactaataa aaaaatttta ttccattaat ctactctata
     3541 tttttcagca tttaagaaat aatttttagt actttgtttg ataatataac taagacaact
     3601 tttcaggaga tgattaaatg acatttaaga ataaaaaatt tactataaat aaatataaaa
     3661 ttctatctat tattctagtt ttatgtttga ttttaaccgc ttttagtagc tatgtgaatt
     3721 atagtcataa aaaatttaaa gattatgttg tcttctatgt tcaacatcaa gatgatgaag
     3781 tgttgtgggc tgggagtgct attgttaatg ctataaaaga aagaggaaaa aatcatgtat
     3841 ttgtggtttt agtatctact ggatgtggta tatccgtctt tgataaatat aaaaaatatg
     3901 aaaacctaac aaatatacaa aaatcagaat atagaaatag agaattttta gcttctctta
     3961 atagccttgg cgtaataagg gaaaatataa tattattacc agaaacaaat acaaatggta
     4021 atactgactt caattacatg gaaaaaattg ctcttacatt tgaagaaaaa tttaaaaatg
     4081 taactcatat agcacatact tataagttag atgaccattt acaacattta aaaaatggtt
     4141 ctgttatcca aaatttatat aatgctggta aaataaaaga tgctaaatat tttgtaaaac
     4201 ctaaatttgc tgataaaata acttttaata ataagatagt ttataaagct cattctactg
     4261 aagactatga aaaggttaaa aatgcctgtc aacaatacaa aattgttgat gaattagaat
     4321 atagagaagg tattggctat aagtctgacc ataaatcatt tgataagtta ttaaataata
     4381 aagatgttcc tgctatactt catacaccta atttataaga aatatttatt cttttaattt
     4441 aagttataaa aaggaaatta tttgagattt tgtgatatag cactttttgt gctatactac
     4501 tttttctcat acaactatta cccatatcta atttcatcat ctcttaaaat atataaagat
     4561 atcatcatta aaacctataa gccttattaa atgctttata catacctaca ttaaactacc
     4621 tgtttatatt aaactacatt aaaataaata tttgttataa attcttaata attatgaaac
     4681 tcatttgtta tttatatttg ttaatatatg tttaaaccta aaaaattaat aaaccaaaat
     4741 aacataagaa gtgtgataga ttaccctaga tattttcata aacttttacc ttcagattaa
     4801 tatgggcatc tatactttta aatttactat atgcattgac aatatattaa aagtactagt
     4861 ttttatttca taatgaatgg gcttactgtt ttctaatacc tctttttggt ataaaataaa
     4921 aattggttaa aaataaaatt tcaagggggt taataagttg aatcaaaata tattggttgt
     4981 agatgatgat agagagatag ttgatgctat agagttctat ttgagacctg ataactttaa
     5041 tgttttaaag gcatatgatg gacttgaagc attggacata ctaatcgaaa atgatattaa
     5101 gttaataata atggatgtaa tgatgcccaa tttagatggg cttaaaacta cacttaaaat
     5161 aagagaaaaa aggaatatac caataattct cttatctgca aaatcagaag atatggacaa
     5221 aattataggt cttaatatgg gtgctgatga ctatataact aaaccattta atccactaga
     5281 acttactgct cgagtaaaat ctcaacttag aaggtatata aatttaggtg attttagtaa
     5341 tacaaataac tcaaatgata ctaattatat tctaaaaagt ggtggcctag agctaaatac
     5401 agatacaaaa atagtttctt tagatggtga agaaattaaa atgacaccac ttgaatataa
     5461 aatacttaca cttcttatgt cacgaaaagg taaaattttc tcttctcatg agatttatga
     5521 aaatgtctgg aatgaggatg cttatagttg tgagagaact attgctgttc atattagaag
     5581 aattagagaa aagattgaaa ttaatccaaa agaaccaaaa tatttaaagg tggtgtgggg
     5641 cattggttac aaaattgaaa aacttaatta ttaatgattc taaagcaaaa tttattttat
     5701 tattattact gtttctatta gtttctatat tatttgaagc agtttatgat ttgaataata
     5761 acttttctaa tattaattat tattatagag agtttgctct cccttatttt tattttttcg
     5821 gtactcctgt acatttgtca aaagtagaat tagagtttgt ctgcataggt attatattat
     5881 caagtttttt attgactata ctatgtttta cattgtataa aaaaaataaa caaactaaag
     5941 tcaaattctt agagaatgtc ataagccttt tttctaaggt aaaaatagaa cttacactta
     6001 tattaacttt gatactatta accttaaggt ttcatgcatt tacaagtaat aaaatatttt
     6061 acaacactaa tttaataatc tacaatttta taattatatc tatagtctat ataatatatg
     6121 aaaatattaa atataataa
//
LOCUS       DBGJFF010000181         6099 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.185, whole genome shotgun sequence.
ACCESSION   DBGJFF010000181 DBGJFF010000000
VERSION     DBGJFF010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6099
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.185"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(404..2236)
                     /locus_tag="ACOIAF_003565"
     CDS             complement(404..2236)
                     /locus_tag="ACOIAF_003565"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2670545.1"
                     /translation="MSRMGRGPMGKSMGAGQKANDFKGTMRKLIAYLSKFKISIILVI
                     VFAIGSASFSIVGPKILGKATTKIFEGLVSKVSGGNVGIDFNAIGKILTFLLCLYLIS
                     ALFSFIQGFIMSGISQKVSYNLRKEISAKLDRLPMKYFDTKTHGEILSRITNDIDTLN
                     QSLNQSMTQLITSVTTMIGVLIMMLSISGIMTLVAVLILPISMFVISRIIKKSQKYFR
                     YQQEYLGNVNGQVEETYSGQTIVKAFNREDEVIEEFDKLNDSLYNSAWKSQFLSGIMQ
                     PLMMFIGNLGYVMVSILGGWLAIKKTIEVGDIQSFIQYVRNFTQPMTQIAQVANLLQS
                     TAAASERVFEFLEEEEEDQIVENAVSIDGLEGKIDFENVNFGYNPNKTIINDFSVNVK
                     PGQKVAIVGPTGAGKTTIVKLLMRFYDVNSGSILIDGHNIKDFNRSELREMFGMVLQD
                     TWLFSGSIMENIRYGKLNATDEEVIEAAKSAHVHRFIKTLPDGYKMKLNEEASNVSQG
                     QKQLLTIARAILADPKILILDEATSSVDTRTEVLIQKAMDNLMEGRTSFVIAHRLSTI
                     RDADMILVMNEGDIVEQGNHEELLKKGGFYANLYNSQFEEDEAM"
     gene            complement(2229..4475)
                     /locus_tag="ACOIAF_003566"
     CDS             complement(2229..4475)
                     /locus_tag="ACOIAF_003566"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2670546.1"
                     /translation="MFKLIKYLKKSALSIVIIVCLLVIQAVCDLSLPEYTSNIVNVGI
                     QQGGVENSVPSVIRESELNKITLFMDKSSKDKVLDNYTLLNKKDYVKYKDKYPGLKDE
                     SLYELNTKDKDTIDDLNVIFGKAILIVSGLEGDSKEVKAMKAQLMSKLPPQATQSGDV
                     DIFKLLSAMPKEQLDTLTKEMSKGFESMPESMITQSSVSYVRSEYEKIGIDTEKTQNN
                     YILFTGAKMLGIAMISMVATITVGFLAARVAASVGRNLRSGVFRKVMSFSNTEMNEFS
                     TASLITRSTNDIQQIQMLMVMLLRIVFYAPIMAIGGVIKVLNTNTSMAWIIAVAVVAI
                     LSLIVVLFSVVMPKFKLVQKLVDKLNLVSREIITGIPVIRAFSTEKHEEERFDKANKN
                     LTKTNLFVNRVMTCMMPAMMLIMNLITVLIVWRGAYSVDSGAMQVGDMMAFIQYTMQI
                     IMSFLMISMVSIMLPRASVSATRIDEVLVSDISIKDPEKSEAQEFKEDKKGLVEFKNV
                     SFMYPDAEEPILTNISFTAKPGETTAFIGSTGSGKSTLINLIPRFFDVTEGEILLGGV
                     NIKNVSQHDLREKIGYVPQKGVLFSGTIESNLKYGRKDATDEEMRTAAEIAQATEFID
                     SKPEAFKTEISQGGTNVSGGQKQRLSIARAIAKNPEVYIFDDSFSALDLKTDAALRKA
                     LKSKTAESTVLIVAQRISTILNAEQIIVLNEGEIVGIGTHKELLKNCEVYEQIALSQL
                     SKEELANE"
     gene            complement(4543..5004)
                     /locus_tag="ACOIAF_003567"
     CDS             complement(4543..5004)
                     /locus_tag="ACOIAF_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HGZ2670547.1"
                     /translation="MNSNSEEKLNDVFLQLMRIYFIRTHFYFDKLGLYHGQPQILFSL
                     KMKDGQSQKELADKRKVKASTTTVMIKRMEKAGLVERKQDEKDQRVSRIFLTDKGLEI
                     CNKVEEFNKELEREFFLGFSEEEKIVLRRLLMQVRDNLKAGCHKHEDEKYF"
     gene            complement(5309..5875)
                     /locus_tag="ACOIAF_003568"
     CDS             complement(5309..5875)
                     /locus_tag="ACOIAF_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class carbonic anhydrase"
                     /protein_id="HGZ2670548.1"
                     /translation="MRKLEEILEYNKSFVENKEYEQYVTSKHPNKKIVVLSCMDTRLT
                     ELLPKAMNLKNGDVKLIKNAGATIMHPFGSIMRSVLVAIYEFDVDEVMVVGHHGCGMC
                     NVDTDKLLGKILDRGISNDVILTLRSAGIDTKQWLHGFDSAEESVKDSIDLIKGHPLI
                     PDGIIVHGLIISPETGKLDVVVNGYEDK"
BASE COUNT         1973 a         1100 c          640 g         2386 t
ORIGIN      
        1 ttatatgtaa ttatcatttt atatttaacc ctattcttaa atttaagtag ttatttgcat
       61 gattaataaa agtaataaaa atatatactt caatacaatc atcaactatt aaaattaaat
      121 tctatttata ataaaattta aagaataaaa ttaaatatac attttgataa tcacctccaa
      181 tattttcctt actatatatt ataataaaat aaacactttg taaagacata aaattatgtg
      241 gctatctcaa aatatagaga atactctttt tcattgttaa aatgctaaaa atacatttta
      301 tttcatacac aaaagagtgc cttatgaact aaaaagttca ttttaagaca ccctcattta
      361 ttttatatta ttaattatat gtctatgcta agatagttgt ttgttacatt gcttcatctt
      421 cttcaaattg agaattgtaa agattagcat agaatccgcc tttctttaga agttcctcat
      481 gatttccttg ttcaactata tctccttcat tcattacaag aatcatatca gcatcacgta
      541 tagttgataa tctgtgggct ataacaaaac ttgttctacc ttccataaga ttatccatag
      601 ctttttgaat caatacttct gttctagtat cgacagagct tgtagcttca tctaatatta
      661 aaattttagg atcagctaaa atagcacgag ctatagtcaa taattgtttt tgaccttgag
      721 aaacattact tgcttcttca tttaatttca tcttatatcc atctggtaat gtcttaataa
      781 atctatgaac atgggcagat ttagcagcct ctataacttc ttcatctgta gcattcaact
      841 taccatatct tatattttcc ataattgaac cactaaataa ccatgtatct tgaagaacca
      901 taccaaacat ttctctaagt tcactacgat taaaatcttt aatattatga ccatcaatca
      961 gtattgaacc gctattaaca tcataaaatc tcattaaaag tttaactatt gtagttttac
     1021 ctgctcctgt aggtccaaca atagcaactt tttgaccagg tttaacattt actgagaaat
     1081 catttattat agtcttgttt gggttgtatc caaagttgac attttcaaag tcaattttac
     1141 cttctaaacc atctattgaa actgcatttt caacaatttg gtcttcttct tcctcttcta
     1201 aaaactcaaa tactctttca gaagctgctg ctgttgattg caataaattg gcaacctgtg
     1261 caatctgagt cattggctgt gtaaagtttc ttacatactg tataaatgac tgtatgtcac
     1321 caacctcaat tgttttctta attgcaagcc agcctcctaa gattgatacc attacatatc
     1381 ccaagtttcc aataaacatc ataagtggtt gcattatacc tgataaaaat tgagacttcc
     1441 atgctgaatt atacaaacta tcatttaact tatcaaactc ttcaatcact tcatcttcac
     1501 gattaaatgc tttcactatt gtttgaccac tgtaagtttc ttcaacttga ccatttacat
     1561 ttcctaaata ttcttgttga tatctaaagt atttttgtga tttctttatt attcttgaaa
     1621 ttacaaacat tgatattggt aatattaaaa ctgccactaa tgtcataatt ccacttatag
     1681 ataacatcat tatcaaaaca cctatcatag ttgttactga tgttataagt tgtgtcatac
     1741 tttgatttaa actttgattt aaagtatcta tatcattcgt aattctcgat aatatttcac
     1801 catgcgtctt agtatcaaaa tacttcattg gcaatctatc caattttgct gaaatctctt
     1861 ttcttaaatt ataggatact ttttgagaaa ttccactcat tataaatccc tgtataaaag
     1921 agaacagtgc acttatcaaa taaagacaaa gtaaaaaggt taaaattttt ccaattgcat
     1981 taaaatctat acctacattc cctccagaaa ctttactcac taacccttca aatatctttg
     2041 tagttgcctt tcctaaaatt tttggaccta ctatagaaaa cgatgcacta ccaatagcaa
     2101 atacaattac tagaattatt gatattttga atttagataa atatgcaatt agttttcgca
     2161 tagtcccttt aaagtcattt gctttttgtc ctgctcccat agattttccc ataggacctc
     2221 ttcccattct actcattagc tagctcctcc tttgaaagtt gtgataaagc tatttgctca
     2281 taaacttcgc aattttttag aagctcttta tgtgttccta ttccaactat ttctccctca
     2341 tttaaaacaa ttatctgttc tgcatttaag atagtgctaa ttctttgtgc aactattaga
     2401 actgtacttt cagcagtttt tgattttaaa gccttacgaa gagctgcatc agtttttaag
     2461 tcaagtgctg aaaaactatc atcaaatata taaacttctg gattttttgc tattgctctt
     2521 gcaatagaaa gtctttgttt ttgacctcca gatacatttg ttccaccttg agaaatttca
     2581 gttttaaaag cttccggttt agaatctata aattctgtag cttgagctat ttcagctgct
     2641 gttctcattt cttcatctgt tgcgtctttt ctaccatatt ttaagttgga ttctatagta
     2701 ccagaaaaca atactccctt ttgaggtaca tatccaattt tttcccttaa atcatgttga
     2761 gaaacatttt ttatattaac tccaccaagt aatatttctc cttcagtaac atcaaagaaa
     2821 cgaggaatta aatttataag agttgactta ccactaccag tacttcctat aaaggcagtt
     2881 gtttctcctg gctttgctgt aaaagatata tttgttaata taggttcttc tgcgtctgga
     2941 tacatgaacg aaacattttt aaattcaaca agaccctttt tatcttcctt aaactcttgt
     3001 gcctctgatt tttctgggtc ttttatgctt atatctgaca ctaatacttc atctatacgt
     3061 gtagctgaaa cggatgctct tggaagcata atagatacca ttgatatcat taagaatgac
     3121 attattattt gcattgtata ttgtataaat gccatcatat ctccaacttg cattgctcca
     3181 gaatcaacac tgtatgcacc tctccaaact ataagaactg tgataaggtt cattataagc
     3241 atcatagcag gcatcataca tgtcataact ctatttacaa aaagatttgt tttagttaaa
     3301 tttttatttg ctttatcaaa tctttcttct tcatgttttt cagtactaaa agctctgata
     3361 actggaatac cagtaattat ttcacggctt actaagttta acttatcaac aagcttttga
     3421 accagtttaa acttaggcat aaccactgag aataacacta caattaatga taagatagca
     3481 actactgcta ctgctataat ccaggccatt gaagtatttg tgtttaaaac ctttatcact
     3541 ccacctatag ccattatagg tgcatagaat actattctaa gcaacataac catcaacatt
     3601 tgtatctgtt gaatatcatt tgtactacga gtaattaatg aagctgtaga aaattcattc
     3661 atttcagtat ttgaaaatga cataactttt ctaaatactc cacttcttaa atttctacct
     3721 acacttgcgg ctactcttgc tgctaaaaat cctactgtta ttgtagctac catactaatc
     3781 attgctatac caagcatttt agcaccagta aataatatat aattattttg agttttttct
     3841 gtatcaatac ctattttctc atattctgac cttacatatg aaacagagct ttgtgttatc
     3901 atactctctg gcatactttc aaagcctttg ctcatctctt tagtcaatgt atctaactgt
     3961 tcctttggca ttgctgataa caatttaaat atatcaacat ctccactttg agtagcttgt
     4021 ggtggaagtt tactcattaa ctgagctttc atagctttta cctctttact atcgccctca
     4081 agccctgata ctattaaaat agctttacca aaaataacat ttaaatcatc tatagtgtct
     4141 ttgtctttag tatttaactc ataaagagat tcatctttta gacctgggta cttatcttta
     4201 tacttaacat aatccttttt attcagcaaa gtataattat ctagtacttt atccttacta
     4261 cttttatcca taaataaagt aattttattt aattcactct ctctaatcac agatggaaca
     4321 gagttttcta ctccaccttg ttgtatacct acatttacta tattggatgt gtattctggt
     4381 aaagaaaggt cacataccgc ttgaattacc aatagacaga ctattattac tattgataat
     4441 gccgatttct ttaaatattt aattaattta aacatacatt tcttcccttc tgtaatatta
     4501 attagtctaa taacattaaa ttgaaaaaca ttattttaaa ctttaaaaat atttttcatc
     4561 ttcatgctta tgacatcctg cttttaaatt atctcgtact tgcattagaa gtcttctaag
     4621 tactattttt tcttcttcac taaatcctaa aaaaaactct ctttccaatt ccttgttaaa
     4681 ttcttcaact ttattacata tttctaaacc tttgtctgtc aaaaaaatcc tagatactct
     4741 ttggtccttt tcatcttgtt ttctttccac aagtccagct ttttccatcc tctttatcat
     4801 aactgtagtt gtagatgctt ttactttcct tttatctgct aattcttttt gactttgccc
     4861 atctttcatc tttaaagaaa aaagtatttg gggttgacca tgatataagc ctaatttatc
     4921 aaaataaaaa tgagttctta taaaatatat tcgcattaat tgcaagaaaa catcatttaa
     4981 tttttcttca gaattgctat tcatacttgc cctccttatt ttttatatta cttagtcagc
     5041 taagtattat tttactatac tgcatgcatt ttttcaatat atttataaaa aattcatata
     5101 aatttcattt attaaatatt tttatatcat atagttataa ttttaataaa taatagtaag
     5161 atttttaatt agtcagctac ttatattttt tattgtaaag cttaaaataa accttttcat
     5221 atattttcaa ataaaaaagt tgtcccaaaa taaattttga aacaacatca tattcaacag
     5281 tatcttgatt atataacatt atgatactct acttatcttc atatccattt acaacaacat
     5341 ctaacttccc agtttcaggt gatataatta gaccatgtac aataatacca tctggaatta
     5401 aaggatgccc ttttattaaa tcaatactat cttttacaga ttcttctgca gaatcgaatc
     5461 catgtaacca ttgttttgta tcaattccag cactacgaag tgtaagtatt acatcattag
     5521 atattcctct atctaaaatt ttaccaagta acttatccgt atcaacatta cacataccac
     5581 atccatgatg ccctacaacc ataacctcat ctacatcaaa ttcgtaaatt gcaactaaaa
     5641 cacttctcat aatacttcca aaaggatgca ttatagtagc tcctgcattt tttattagtt
     5701 tcacatctcc atttttcaaa ttcatagcct taggcaaaag ctcagttaat cttgtatcca
     5761 tacaagataa gactactatt tttttatttg gatgttttga tgtcacatat tgttcatatt
     5821 ctttgttctc tacaaatgat ttattatatt ctaatatctc ttcaagtttt ctcatatgta
     5881 atatcaccct ctttaaaaat ttttttatta ttatatcact attaaagtac aatatataca
     5941 catataattt aatttatatt tattaaatta ttttataaga ttattactct actataaatc
     6001 ttgtatataa cttgtattta taagtatttg tatatttatt ttttataatc taaactaata
     6061 ttatatattt tcattatttt tttaattata attttttta
//
LOCUS       DBGJFF010000182         6093 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.186, whole genome shotgun sequence.
ACCESSION   DBGJFF010000182 DBGJFF010000000
VERSION     DBGJFF010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6093
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.186"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            209..1378
                     /locus_tag="ACOIAF_003569"
     CDS             209..1378
                     /locus_tag="ACOIAF_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670549.1"
                     /translation="MEKLTDRRQVKAVSMETFIFLILLVVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLETVFLIMAMAVLAGALSALLSEFGVIALINKIFAVFMKPLYGLPGASIA
                     GAITTYLSDNPAIIPFAKDKTFTQYFKQYQVPALCNLGTAFGMGLILTTFMISQGTEY
                     VLPALIGNLGAIIGSVISVRIMLTFTKKFYKYNPEEDKATGTLEKKDEFREIREGNVF
                     QRALDAILEGGKMGVDMGMAIIPGVLVVCTLVMLLTFGPSTDPVTGQEVYTGAAYEGI
                     KLLPVIGDKLGFILEPLFGFTSPEAIAFPITSLGAVGAAMSLVPEFIKSGAITPNDIA
                     VFTAMGMCWSGYLSTHIGMMDALNARQLAGKAILSHTIGGLCAGAAAHFIFTLVG"
     gene            1758..6011
                     /locus_tag="ACOIAF_003570"
     CDS             1758..6011
                     /locus_tag="ACOIAF_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HGZ2670550.1"
                     /translation="MDELFHIGIDVGSTTVKVVVLNNSNNIVHKEYRRHYSDVKKSVK
                     EVLNGIYEKLGDINTTIIITGSGGIGISKKLGVKFVQEVISSTKSIEYFHPETDVVIE
                     LGGEDAKITYLSGGIDQRMNGICAGGTGAFIDQMASLLKTDASGLNELAKGYNVIYPI
                     ASRCGVFAKTDIQPLINDGANQTDIAMSIFNAVVVQTVSVLSCGRKIEGNVAFLGGPL
                     YFLSELREAFKRVLDLKDENIIFPENAQLYIAIGAGLLSVDEDNIQLKSLIEKLDSIK
                     NIEDGEVNLLEPLFKDKNEYEEFSRRHEKEKINYVDINSIKSNCYLGIDAGSTTTKAA
                     LIDEDGRLVYSYYNSNEGNPLKTTIKVINEVYDILPKNIKILSSTVTGYGEGLIKKAL
                     KIDNGEIETIAHYKAAKFFNKDVDFILDIGGQDMKCLKIKDGVIDNIILNEACSSGCG
                     SFLETFASSLSMTIEEFAHEGIYSQNPVDLGSRCTVFMNSRVKQAQKEGASVGDISAG
                     LSYSVIKNALFKVIKIRDLNEIGNNIVVQGGTFYNDLVLRSFEKLIGKNVIRPNISGI
                     MGAFGSALIAKEKYESGYKTSLLSKEELNNIKLEASVTRCKGCSNHCLLTINKFSDNE
                     IFISGNRCEKGEAIYGDKKIGLEKKKPINLFKYKYNRIFRYKPLEKEEAKNGEIGIPR
                     VLNMYEDYPFWFTFFNAMGFRVVLSDRSSKQLYETGITSIASETVCYPGKLVHGHIEN
                     LIAKGIKNIFYPSVTNENKEDSNADNYYNCPVVISYSEVIKNNVENIRNKNINYINPF
                     ISLNDKEKLKKRLYDELSRHFSDIKITKEEVNHAVDKATDEQNLFKMEIQAAGERALK
                     EIKERNMKGIVLCGRPYHIDPEINHGMPELINSLDMAVLTEDSICHLANVQRPLRVVD
                     QWVYHSRLYKAASFVRDKNYLELVQLNSFGCGLDAVTTDQVQEILNEKSKIYTIIKID
                     EGNNLGAAKIRMRSLKAAMFERESKNIDVKNIKVNRIQYAKNNQITNKHTILAPQMSP
                     IHFQFLEKAVNLSGYNIEILKDTDSSVIEEGLRYVNNDACYPAIIVVGQLITALKSGK
                     YDLNNTSVTITQTGGGCRATNYIGFLRKAIYDAGFKDIPVIALSVNGIEDSGIMDNIS
                     LKLINRLFMSVVYGDLLMKVLYRVRPYEKVTGSANALYEKWVDICKSSLVKAKISVFT
                     RNIKDIVKEFDNLEILDIKKPKVGLVGEILVKFHPIANNNLVDILEREGAEAVVPDLT
                     NFFLSCAFNTIYKHTHLEGSRKSRMIGEAFIYITGIYQRVYKKALDKSERFYAPANIK
                     NVAKSTEPVVSLGNQTGEGWLLTGEMVELLNEGVENIICMQPFGCLPNHIIGKGSIKE
                     LKRLYKNANIIPIDYDPSASEVNQLNRIKLMLSKAFKNI"
BASE COUNT         2409 a          672 c         1119 g         1893 t
ORIGIN      
        1 aggaatcata ctttttataa aatgaaagat aagatgtcga aaacttgcat aaatctgaaa
       61 aatttttatt attaaaatac tgatttccat tttaaaaatg ataaaaaaat gacaaataaa
      121 gttgcaaata gtaatactta taagtataat taaaaaagag aaaaggtttt gttaaacttt
      181 ataacaaaaa aagaagggat gattcaaaat ggagaagtta acagacagga gacaggttaa
      241 ggcagtaagc atggaaacct tcattttcct aatattacta gtagttggtt ttgggtatgt
      301 tggtagtatt atgggagcag gaatgatgtt caaagtaata atgagtacag cacatgcttt
      361 actattggag acagttttct taattatggc tatggcagtt cttgcaggcg cattaagcgc
      421 actactttca gagtttggag taatagcgtt aattaataaa attttcgctg tatttatgaa
      481 gcctttatat ggactacctg gtgctagtat agcaggagct ataacaactt atttatcaga
      541 taatccagct attatacctt ttgctaaaga taaaacattc acacagtatt ttaaacaata
      601 ccaagttcca gcattatgta acttaggaac agcctttggt atgggactta tcttaacaac
      661 atttatgatt tctcaaggta cagaatatgt acttccagca ttaattggta atttaggtgc
      721 aattataggt agtgtcataa gtgttagaat aatgttgaca tttactaaga agttttataa
      781 atacaatccg gaagaagata aagcaacagg aacattagag aaaaaggatg aatttagaga
      841 aatcagagaa ggaaatgtat ttcaaagagc tttagatgca attcttgaag gtggtaaaat
      901 gggggtagat atgggaatgg ctataattcc tggagtccta gtagtatgta cattggtgat
      961 gttattaact tttggaccat ctacagatcc tgttacaggt caagaagttt atacaggagc
     1021 tgcatatgaa ggaataaaat tacttccagt tattggagat aaacttggat ttatattaga
     1081 accattattt ggatttacat caccagaggc aatagcattc cctataactt cattgggagc
     1141 tgttggcgca gcaatgtctc tagttccaga gtttataaag tcaggagcta taactcctaa
     1201 tgatatagca gtatttacag ctatgggtat gtgttggagt ggatatttaa gtacacatat
     1261 tggtatgatg gatgcattaa atgctagaca gttggcaggt aaagctattc tttctcatac
     1321 aataggtggt ttatgtgcag gagcagctgc tcactttata tttacactcg taggataaat
     1381 gtaatcagct aagataaaaa ataatataca aaaaatggtt gttgatatgt aataagaatt
     1441 tacatatcaa caaccatttt tttctgacta aagataattt ataatacata aacattaagt
     1501 ggatattaga gttaagttat atttatgtaa aaagtaacaa aaatctgtag aaatttatga
     1561 caaaatttag tacactaact atatgttctt atttaaaatt taaggttaga attaggaatt
     1621 acctagtaga gtaagctttg attaataaaa gaatcataaa ataaaaatat tagtcataaa
     1681 gattataaat aacttactta aaataaagcc atagaactta caaaaattaa aaaagcatat
     1741 aaatatggag gggtacaatg gatgaattat ttcatatagg aattgatgta ggttctacaa
     1801 cggtgaaggt tgtggtactt aataatagta ataatatagt tcataaagaa tatagaagac
     1861 attattctga tgtaaaaaaa tcagttaaag aagtactaaa tggtatatat gaaaaattgg
     1921 gagatataaa tactaccatt ataataactg gttcaggtgg aattggaata tcaaaaaaat
     1981 taggtgtaaa atttgttcag gaagttatat ctagtacaaa atcaatagag tattttcatc
     2041 ctgaaactga tgttgtcata gaacttgggg gtgaagatgc aaagataaca tatctaagtg
     2101 gaggaataga ccaaagaatg aatggaatat gtgcaggtgg cactggagca ttcatagacc
     2161 agatggcatc acttttaaag acagatgcca gtggattaaa tgaacttgct aaagggtata
     2221 atgttatata tcccatagct tcaagatgtg gtgtttttgc taaaacagat attcaacctc
     2281 ttataaatga tggagccaat cagactgata tagcaatgag tattttcaat gcagttgtcg
     2341 tacaaactgt gagtgtcctt tcttgtggaa gaaaaataga aggaaatgtt gcgtttttgg
     2401 gtggtccatt gtacttttta tctgaactta gagaagcatt taaaagagta ttagatttaa
     2461 aagatgaaaa cataatcttc ccagaaaatg cacaacttta tattgctatt ggagcaggat
     2521 tattatcagt agatgaagat aatatacaac taaaatctct aatagaaaaa ttggatagta
     2581 taaagaatat tgaagatgga gaagtaaatt tattagaacc tttatttaaa gataaaaatg
     2641 agtatgaaga attttcaaga agacatgaaa aagaaaaaat aaattatgtg gacataaatt
     2701 caataaaaag caattgttat ttaggaattg atgcaggctc aactacaact aaagcagcac
     2761 ttattgatga ggatggaaga cttgtatatt cttattataa tagcaatgaa ggaaatccat
     2821 taaaaacaac cattaaagtt ataaatgaag tatatgatat attaccaaaa aatattaaaa
     2881 tattgagttc aactgtaact ggatatggag aaggtcttat taaaaaagct cttaaaatcg
     2941 ataatggaga gatagagact atagcacatt acaaagctgc taaatttttc aacaaagatg
     3001 tagattttat acttgatatt ggtggtcaag atatgaagtg cctaaaaata aaagatggag
     3061 taattgataa tataatatta aatgaggctt gttcatcagg ttgtggctca tttttagaga
     3121 catttgcaag ttctttatct atgacaatag aagaatttgc gcatgaaggt atttattctc
     3181 aaaatcctgt agatttaggt tcaagatgta ctgtatttat gaattctaga gtaaaacaag
     3241 ctcaaaaaga aggtgcaagt gttggagata tatcagcagg gctatcttat tcagtaataa
     3301 aaaatgcttt atttaaagta ataaagatta gagatttaaa tgagattggt aataatattg
     3361 ttgtacaagg tggaaccttt tataatgatt tggtgcttag aagctttgaa aaactaattg
     3421 gaaaaaatgt aattagacct aatatttcag gaattatggg agcttttggt tcagcactta
     3481 tagctaaaga aaaatatgaa agtggataca aaacatcctt gttatctaag gaagagttga
     3541 ataatatcaa gctagaagca agtgtaacta gatgtaaagg ttgttcaaat cattgtcttc
     3601 ttacaataaa taaattttct gataatgaaa tttttatttc aggtaataga tgtgagaaag
     3661 gtgaagctat ttatggtgat aaaaaaatag gattagaaaa gaaaaaacca ataaatttat
     3721 ttaagtacaa atacaataga atttttagat acaaaccatt ggaaaaagaa gaagcaaaaa
     3781 atggggaaat agggatacca agagtattaa atatgtatga agattatcca ttttggttta
     3841 cttttttcaa tgctatgggc tttagagttg tactttcaga taggtcatca aaacagttat
     3901 atgaaactgg tataactagt attgcatctg aaacagtttg ttatccagga aaattagttc
     3961 atggacatat agaaaattta attgcaaaag gtataaaaaa tatattttat ccaagtgtaa
     4021 caaatgaaaa taaagaggat tcaaatgcag ataattatta taattgtcct gttgtaatat
     4081 catattctga agtaataaaa aacaatgtag agaatataag aaataagaac ataaattata
     4141 taaatccatt tataagtcta aatgacaagg aaaaattgaa gaaaagactc tatgatgaat
     4201 tatcaagaca tttttctgat ataaaaataa ctaaagaaga agttaatcat gcagttgata
     4261 aagctactga tgaacagaat ttatttaaaa tggagataca agcagcaggt gaaagagcac
     4321 ttaaagaaat aaaagaaaga aatatgaagg ggattgtgtt atgtggaaga ccatatcata
     4381 tagacccaga aataaatcat ggaatgccag agcttataaa ttcattagat atggcagtgc
     4441 ttactgaaga ctctatatgt catttagcca atgtacaaag accacttaga gttgtagacc
     4501 aatgggtata tcattcaaga ttgtataaag cagctagttt tgtaagagat aaaaactatt
     4561 tggaacttgt tcaacttaat tcatttggct gtggattaga tgcagtaact acagaccagg
     4621 ttcaagaaat acttaatgaa aaatcgaaga tatatactat tataaagatt gacgaaggca
     4681 acaatttagg agcagctaag ataaggatga ggtccttaaa agcagctatg tttgagagag
     4741 aaagcaaaaa tatagatgta aaaaatataa aggtaaacag gatacagtat gctaaaaata
     4801 atcagataac gaacaaacat actatattag caccacaaat gtctccaata catttccaat
     4861 tccttgaaaa agctgttaac ttaagtggtt ataatataga aattctaaaa gatactgata
     4921 gtagtgtaat tgaagaaggg ttaagatatg taaataatga tgcatgttat ccagctataa
     4981 tagttgtagg tcagctaata acagcattaa aatctggcaa atatgattta aataacactt
     5041 ctgttactat aactcagact ggtggaggtt gtagagctac aaattatatt ggattcttaa
     5101 gaaaggctat atacgatgct ggatttaaag atattcctgt cattgcactt agtgtaaatg
     5161 gaatagaaga tagtggtata atggataata tatcactaaa attaatcaat agactcttta
     5221 tgtctgttgt ttatggtgat ttgctgatga aagtactata tagagtaaga ccatatgaaa
     5281 aagttactgg aagtgcaaat gctctatatg aaaaatgggt tgatatatgc aaatcttctc
     5341 ttgttaaagc aaaaatttct gtatttacta gaaatataaa agatatagta aaagaatttg
     5401 ataatttaga gatactagat atcaaaaaac caaaagtagg tttggttgga gaaatattgg
     5461 tgaaattcca tccaattgca aataataatt tagtagatat attagagcga gaaggtgctg
     5521 aagcagtcgt gccagacctt acaaatttct tcttaagttg tgcctttaat actatataca
     5581 aacacactca tttagaagga agtagaaaga gtagaatgat aggagaagca tttatataca
     5641 taactggaat atatcaaaga gtatataaaa aagcacttga taagagtgag agattttatg
     5701 ctccagccaa tataaaaaat gttgctaaaa gtacagaacc agtagtgtca ttaggtaatc
     5761 aaactggaga aggttggctt ttaacaggtg agatggttga attattaaat gaaggtgtag
     5821 aaaacattat ttgtatgcaa ccttttggat gtttacctaa tcacataatt ggtaagggtt
     5881 ctataaaaga attaaagagg ttgtataaaa atgcaaatat aatacctatt gattatgacc
     5941 cttctgcaag tgaggtaaat caacttaaca ggataaaact tatgctttct aaggctttta
     6001 aaaatatata agatttatat tttgatatgt taaaatttaa tgaatttaaa aatatactct
     6061 atattaagta tctataaaaa agagttatcc tat
//
LOCUS       DBGJFF010000183         5984 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.187, whole genome shotgun sequence.
ACCESSION   DBGJFF010000183 DBGJFF010000000
VERSION     DBGJFF010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5984
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.187"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            312..1361
                     /locus_tag="ACOIAF_003571"
     CDS             312..1361
                     /locus_tag="ACOIAF_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HGZ2670551.1"
                     /translation="MKKIRKLATKLLITTMVLISGMSMTAYGMTAKEVTTKTPKSYVT
                     GTNSVYGPKLSQAQLNSVAQATADFMNKKITKNMTTDAKILVAYNHIKNNTTYVDWNA
                     VEGANTAYTLVTKKGACSGMARSMKALCDAMGIECYYVHSTSNDHQWNLIRFGDRRLY
                     HVDIDANKSAGKDIIYKSLTHPLPFDKTAYPKVGAIVQTVKPEQTQKPAPPGKHTMLP
                     HQEGAKAFGELGDIKDKHVMYEGVRCIVINSSVNTVTFIGSKSNLDPSKLVIAVIGFM
                     NTPYGMFALAPEGNPVEYPFEYDKPFSLDFSGKNRSGVELAKYCKKNDVTIKIEITDK
                     EANNGIGQVMYIRYE"
     gene            1703..4057
                     /gene="yicI"
                     /locus_tag="ACOIAF_003572"
     CDS             1703..4057
                     /gene="yicI"
                     /locus_tag="ACOIAF_003572"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: protein motif:HMM:NF007940.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HGZ2670552.1"
                     /translation="MKFSNGMWLNKENYTVLHPVELYDMCIEEVKLTAYCPFVKVNTR
                     GNTLDGGMLTTEISSPMENIIKVRITHHAGEKEQSYFKLNEKTPDIKIKENNDNYEFI
                     SGRLKAQIGKGENWGIKFYNNDKLLTETSRTGTAYITTDKKEIYMREQLAIGVGELVY
                     GLGERFTPFVKNGQSVDIWNQDGGTASEQSYKNIPFYLTNKGYGVFVNHSENVSFEVG
                     SESVSKVQFSVEGESLEYYIIGGNDLKEVINNYTNLTGKPTLPPAWSFGLWLTTSFCT
                     NYDEETVMGFIDGMLERDIPLETFHFDCFWMKEFEWCNFEWDNRVFPDPEGMLKRIND
                     KGIKVCVWINPYIGQKSPLYKEGAEKGYFVKTGNNKIWQCDRWQAGMALVDFTNPEAC
                     KWYKSHLERLIDMGVDSFKTDFGERIPVQDEFYGMDALKYGIKYYDGSDSKKMHNYYT
                     YLYNKLVFELLVEKLGNEQACVFARSATVGGQNFPVHWGGDCLSNYPSMAESLRGGLS
                     LSLCGFGYWSHDIGGFEEGCTADIYKRWTQFGLLSSHSRYHGNVEYKVPWIYDEEAVE
                     VTRKFAKLKMKLMPYLFKYVVEAKNTGIPMIRPMVLEYSDDETCIYLDRQYMFGEGLL
                     VAPIFSDTGDVSYYLPEGRWTNLLNNKVLEGRRWYKENHDYMSLPLMVKDNTIIPIGY
                     NDKKPDYDYLKDLEINIFELDENNRIETILYNSLGKGVGKVSAIKIGNKISVQTKGIS
                     GDYKIVLRNINATSENAKCKYDLEIGTVAQAKDSEVIFTVKNNF"
     gene            4137..5501
                     /locus_tag="ACOIAF_003573"
     CDS             4137..5501
                     /locus_tag="ACOIAF_003573"
                     /inference="COORDINATES: protein motif:HMM:TIGR00792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HGZ2670553.1"
                     /translation="MENNMKQPIIASDKLSGKEKVGYATGDLACNLIFSTVGAFLMYF
                     YTDVYGLAPMAVSMIFLVARIWDGLNDPIMGVIVDRTKSKHGKFRPYLLYGALPFTLI
                     AILCFTVPDISPMGKFVYAFVTYIGLGMIYTLVNVPYGALTSAMTQDSTERANLSVVR
                     MFFASVGTLIVTYGVPKLSTILGGENLAKGYQQAMIIYGIAGGLLLLFCFFTTKERYS
                     VVNEDSEKAGFKDIIVLLKNNKPLVILSLIFILMFGIMSIVDAVGIYFFQYVLGREDL
                     LPTYMILGKGAVLVGYAVTPLLLKKFDKKEIIFWFTVVTLSLPIGMMFLGKEHITLIM
                     ILRVIHGIAYSSTGIMWSLVPDTIEYGELKTGKRREGITYSIIGFFFKFGMALGGLVP
                     GVVLSMTGYVANTSQTSQALYGIRSINGIIPVVLTVAMLVLIKMYPLTEEKCREVINE
                     LRSR"
     gene            complement(5603..>5984)
                     /locus_tag="ACOIAF_003574"
     CDS             complement(5603..>5984)
                     /locus_tag="ACOIAF_003574"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGZ2670554.1"
                     /translation="SISYKNTDKYFDRLKIITDYVKENYKEQISLSTASKVVNLTPQY
                     LSRFFKTHMGITFTSYVNNIRLENMYSDLINTDNNILDIIQNNGFYNYKQFMKLFKKI
                     YNCTPSQKRQQINKTRKDVVSKYR"
BASE COUNT         2216 a          710 c         1111 g         1947 t
ORIGIN      
        1 attgataatt ctctttattc ttaccattac taatgattga aaatagtact atttattata
       61 ggtcaaagat tccgaatata ttaatcatca tcattttatc aaactaacag caaacacact
      121 gatttgcgag ttgtattgta tacaaattat ctagaaaata tctattagat tttcaatgaa
      181 atttaaatat ttcacgaacg tatgaatgga gaaatataca tgttgacaat ccaccttttt
      241 aaatcctaaa ttaattttat taatggtaga gaataggaag taacaatgtt ttataaagga
      301 gaatattaag tatgaagaaa attaggaagt tagctaccaa gttattgatt acaacaatgg
      361 ttttaatctc aggtatgagt atgacagctt atggtatgac agccaaggag gtaacaacga
      421 agacaccaaa aagctatgtc actggaacta atagtgtata tggacccaag ctgtcacaag
      481 cccaactgaa ctcagtagcg caagcaacag cagattttat gaacaagaaa atcacaaaga
      541 atatgactac agatgctaag attttagttg cctataatca cattaaaaat aacacaacat
      601 atgttgactg gaacgcagta gagggagcca atactgctta tacattggtt actaaaaagg
      661 gagcatgttc tggaatggct aggagcatga aagctttgtg tgacgctatg ggaattgaat
      721 gttattatgt ccatagtaca tcaaatgacc accaatggaa tcttatccgt tttggtgata
      781 ggcgtttgta tcatgtagat attgatgcta ataaatcagc aggaaaggac attatataca
      841 agagccttac acatccattg ccatttgata agacagcata tccaaaagtt ggtgcaatag
      901 tacagacggt aaaaccagaa cagacacaga aaccagcacc acctgggaag cacactatgt
      961 taccacatca agaaggggca aaggcttttg gagaattggg agatataaag gacaaacatg
     1021 taatgtatga aggtgttcga tgtattgtaa ttaattcatc agtaaataca gttacattta
     1081 taggtagtaa atcaaattta gacccatcaa agcttgtgat tgctgtaata ggatttatga
     1141 acacaccata tgggatgttt gcattagcac cagaaggtaa tcctgttgaa tatccttttg
     1201 aatatgataa gccattttct ttagatttta gtggtaaaaa tcgttctggt gtagaattag
     1261 cgaagtactg caaaaaaaac gatgtaacta ttaaaataga aatcactgat aaagaagcta
     1321 ataatggaat tggtcaagta atgtatatta gatatgaata aattataaac attgtatcct
     1381 atcactgggc tgatagatta gggattctta ggaataccct ttattttttt gtataaaaaa
     1441 ggagatgatt caaattttta ttttgagata tctccttttt tagataattt gtaataatca
     1501 tgagtttaaa tttaaaaata aatatgtaaa atgggatttt aaaatattac atgagtagct
     1561 ttcctctaaa catttttata aataaaaagc taaaaaagac ctataaaata gcatagaaaa
     1621 tacctaatat atctctaaaa aataatataa actatactta agttgaagca aagctaatat
     1681 tagttgaagg aggtatatta aattgaaatt tagtaatgga atgtggttaa acaaagaaaa
     1741 ttacacggtt ttacatcctg ttgagcttta tgatatgtgt atagaagagg taaaattaac
     1801 agcatattgt ccgtttgtca aagtaaatac aagaggaaat actttagatg gaggaatgtt
     1861 aacaacagaa atatcttctc ctatggaaaa tataattaag gtaagaatta cccatcatgc
     1921 aggagaaaag gaacaatctt acttcaaatt aaatgaaaaa acaccagata ttaaaataaa
     1981 agaaaataat gataactatg aatttatatc aggtagatta aaagcacaaa taggaaaagg
     2041 ggaaaactgg gggattaaat tttataataa tgacaagtta ttaactgaaa caagtagaac
     2101 aggtactgct tatattacaa cagacaaaaa agaaatttat atgagagaac aattagcaat
     2161 aggagttgga gagttagtat atggtttggg agaacgtttc accccttttg taaaaaatgg
     2221 tcaatcagtg gatatttgga atcaagatgg tggaactgca agtgaacagt catataaaaa
     2281 tataccgttt tatttaacaa ataaggggta tggagttttt gttaatcatt ctgaaaacgt
     2341 atcttttgaa gtaggctcag agtcagttag taaagttcaa tttagtgtag aaggtgaatc
     2401 acttgaatat tacataatag gtggaaatga cctaaaagag gttataaata attatacaaa
     2461 tttaactgga aaaccaacac ttccaccagc gtggtcattt ggtctttggc taacaacttc
     2521 tttctgtaca aattacgatg aagaaacagt aatgggcttt atagatggaa tgttagaaag
     2581 ggatatacca cttgaaactt tccactttga ttgtttctgg atgaaagaat ttgagtggtg
     2641 taactttgaa tgggataata gagtatttcc agacccagaa ggaatgttaa aaagaattaa
     2701 tgataagggt ataaaagttt gtgtttggat aaatccatac ataggtcaaa aatcacctct
     2761 atataaagaa ggtgctgaaa aaggatactt tgttaaaact ggaaataata aaatatggca
     2821 atgcgataga tggcaagcag ggatggcctt agttgatttt actaatccag aggcttgtaa
     2881 gtggtataag tcacaccttg aaagattgat tgacatggga gtagatagtt ttaaaactga
     2941 ttttggtgaa aggatacctg ttcaagatga attttatgga atggatgcac taaaatatgg
     3001 cataaaatac tacgacgggt cagattctaa gaaaatgcat aattactata cttatttata
     3061 caataagcta gtatttgaac ttttagtaga gaaactagga aatgaacaag catgtgtgtt
     3121 tgctagatca gcaacagttg gaggacaaaa cttcccagta cattggggag gagactgttt
     3181 gtccaattat ccttctatgg cagagtcatt aagaggtgga ttatctttaa gtctttgtgg
     3241 atttggatat tggagtcatg atataggagg atttgaagaa gggtgtacag ctgatattta
     3301 caaaagatgg actcaatttg gtcttttatc atcacatagt agatatcatg gaaatgttga
     3361 atataaagta ccctggatat atgatgagga agctgttgaa gtgactcgta aatttgctaa
     3421 attaaaaatg aagcttatgc cttatctatt taaatatgtt gttgaagcaa aaaatactgg
     3481 tatacctatg ataagaccta tggtacttga gtatagtgat gatgaaacct gtatatacct
     3541 agaccgtcag tatatgtttg gtgaaggttt attagtagct cctatattta gtgatactgg
     3601 agatgtaagt tattatttgc cagaaggaag atggacaaat ttattaaata ataaagtact
     3661 tgaaggtaga agatggtaca aggaaaatca tgattatatg tcactgcctt taatggttaa
     3721 agataatacc ataattccta taggatataa tgataaaaaa ccagattatg attatttaaa
     3781 ggatttagag attaacatat ttgaattaga tgaaaataat agaatagaaa cgatactata
     3841 taactcactt ggaaaaggag taggaaaagt atctgcaata aaaataggaa ataaaataag
     3901 tgtccaaaca aaaggaataa gtggagatta taaaatagtt cttagaaaca ttaatgcaac
     3961 atctgaaaat gctaagtgta agtacgattt agaaatagga acagtagcac aagctaaaga
     4021 tagtgaagtc atatttacag taaagaataa tttttaagta tttataacta gatagtgttg
     4081 ttataaagtg agttagtatc aatttatacc agctctaaga taagggggtt aattacatgg
     4141 aaaacaatat gaagcaacct attatagcaa gtgacaagct ttcagggaaa gaaaaggtcg
     4201 gttatgctac aggggattta gcatgtaact taatatttag tacagttgga gcatttttaa
     4261 tgtattttta tacagatgta tatggattag ctccaatggc agtatctatg atttttttag
     4321 tagcaaggat ttgggatggt ttgaatgacc ctataatggg tgttattgta gatagaacaa
     4381 aatcaaagca tggtaaattt agaccatatc ttttatatgg tgcattgccg tttacattaa
     4441 tagctatttt atgtttcaca gtgccagata tatctcctat gggtaaattt gtatatgcat
     4501 ttgttactta tatagggtta ggtatgatat atacgttagt aaatgtacct tatggagcat
     4561 taacatcagc aatgacacaa gattctacag aacgtgcaaa cctttccgta gtaagaatgt
     4621 tctttgcatc agtaggaact cttatagtaa cttacggtgt tcctaagtta agtacaatat
     4681 taggaggaga aaatttagct aagggttatc aacaagcaat gataatttat ggtatagcag
     4741 gtggattatt actattattc tgtttcttca ctactaaaga aagatatagt gtggtaaatg
     4801 aagatagtga aaaagctggg tttaaggata taatagtttt acttaaaaat aacaagccat
     4861 tagttatatt gtcattaata ttcatattga tgtttggtat tatgtctata gtggatgctg
     4921 tgggtatata tttcttccaa tatgtattag gtagagaaga cttacttcca acatatatga
     4981 tactaggaaa aggtgctgta ttagttggat atgctgttac tccacttcta ttaaagaaat
     5041 ttgacaagaa agaaataata ttttggttta ctgtagttac tttatctctt ccaattggaa
     5101 tgatgttctt gggaaaagaa catataactt taattatgat tcttagagta attcatggta
     5161 tagcttattc tagcactgga ataatgtgga gcttagttcc agatactatt gaatatggtg
     5221 aattaaaaac aggaaagaga agagaaggta taacttattc tataatagga ttcttcttta
     5281 agtttggtat ggctttaggt ggattagttc caggggtagt attatctatg acaggatatg
     5341 ttgcaaatac ttctcaaact tcacaagctt tgtacggtat tcgttctata aatggcatta
     5401 taccagtagt acttacagta gctatgttag tacttataaa gatgtatcca ctaactgaag
     5461 aaaaatgtag agaggtaata aatgaattaa gaagtagata atatataaag gagtgtctca
     5521 aagtgaactt tttagttcat gagacactct tttttatatt aaatcaagtc tattttaatc
     5581 atctcaataa cgaaaaatag acttatctat attttgagac aacgtctttt ctagttttat
     5641 ttatttgttg ccgtttttga cttggtgtac aattatatat tttcttaaat aatttcataa
     5701 attgtttata attataaaat ccattgtttt gaataatatc taatatatta ttatctgtat
     5761 ttataaggtc actatacata ttttcaagtc ttatattatt tacataagag gtaaacgtta
     5821 ttcccatatg tgttttaaaa aatctagata aatattgagg tgttaaattt acaaccttac
     5881 ttgctgtaga tagagaaatt tgttctttgt aattttcttt tacataatca gttataattt
     5941 ttagtctatc aaaatattta tctgtatttt tataagatat tgaa
//
LOCUS       DBGJFF010000184         5947 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.188, whole genome shotgun sequence.
ACCESSION   DBGJFF010000184 DBGJFF010000000
VERSION     DBGJFF010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5947
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.188"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(255..848)
                     /locus_tag="ACOIAF_003575"
     CDS             complement(255..848)
                     /locus_tag="ACOIAF_003575"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HGZ2670555.1"
                     /translation="MVGAVAGLIVLLYRILLGQAGTWLSQILKFVEGSTIKIIAWFAV
                     LAVLAWIVSKLVDWEPMISGSGIPQLEGEMTGKIKQVWWRVLPTKFIGGFLSLMGGLA
                     LGREGPSIQLGAMAGKGISRALDRGKTEEKFLLTCGASAGLSAAFHAPLAGVMFSLEE
                     VHKNFSVSVLISVMTASLTADYISANFLGMESVFQFD"
     gene            complement(1080..1616)
                     /locus_tag="ACOIAF_003576"
     CDS             complement(1080..1616)
                     /locus_tag="ACOIAF_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HGZ2670556.1"
                     /translation="MIGLKCGMVKLLEHQMIWDKSAKDVIILLKSIWDKTAIDIQHIG
                     STSIPSISAKPIIDIVVGVASLEEAKLYLERLEQCGIVFRGQDVPKQLLFAMGDFEKN
                     TRTHHIHVVEWNSVAWNNYINFRDYLNTFTDKAKLYDSHKQKLALQFEDNRKIYTQRK
                     QELINILLEEARLWRINT"
     gene            complement(1689..2102)
                     /locus_tag="ACOIAF_003577"
     CDS             complement(1689..2102)
                     /locus_tag="ACOIAF_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003507530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HGZ2670557.1"
                     /translation="MKMPKESIDTIMFAPCGMNCMVCYKHCYHKKPCAGCLNGNTGKP
                     EHCRKCKIKDCVCQKGLFYCFECFNFPCKLIKNLEKSYNKRYQASLMENSKFVQQYGL
                     EKFMQKQKERYTCSKCGGIISIHDRECSECQEKVK"
     gene            2568..3164
                     /locus_tag="ACOIAF_003578"
     CDS             2568..3164
                     /locus_tag="ACOIAF_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5082 family protein"
                     /protein_id="HGZ2670558.1"
                     /translation="MDEKILELLQEMQGNISNMQGSISGIEGTISDMQSNINGIEDTI
                     SGMQGTISDMQGSISGIDDTISDMQGSISGMNGTISDMQGSISSMDGTINSMQDSITD
                     MQVEIKEVNSRLDNMEPDIKDLKTGQVETSSRLDTIQSDVTEIKSDVKINRNTLDTVI
                     KHTSSLVKDTSDIKNDLNNIEIVTSKNSNDIAKLKSVK"
     gene            complement(3772..3975)
                     /locus_tag="ACOIAF_003579"
     CDS             complement(3772..3975)
                     /locus_tag="ACOIAF_003579"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGZ2670559.1"
                     /translation="MCLDAVIYMLQTGKQGADKKYLDNMFFVKGYVEELLFLDNSYDI
                     VFSCIVFHHCTNVQNIFCIFWKW"
     gene            complement(4177..4710)
                     /locus_tag="ACOIAF_003580"
     CDS             complement(4177..4710)
                     /locus_tag="ACOIAF_003580"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HGZ2670560.1"
                     /translation="MQYISYYHSPIGNILLASDNIGLTGLWFENQKYYAYNLDQEHQE
                     KELPIFEQTKKWLDIYFSGKEPNFTPSLHMVGTPFQTTVWKILQQIPYGKTITYGEIA
                     YKVAQQKGTSKMSAQAIGGAVGHNGISIIIPCHRVVGTNGSLTGYAGGIDRKIKLLSL
                     EKVSVQHYFIPKKGSAL"
BASE COUNT         2138 a          995 c          736 g         2078 t
ORIGIN      
        1 ttacctgctg attgcccatt ttaaagtact tagggtttaa ccttatacca tctaaccaat
       61 tttttctact ttcgtaactt tcacacttag gtttatttca tccttatgtt ttagtttagt
      121 tagctttagg ggtttccagc aattcaagta gtattggata ggttctatcc tatctctaca
      181 tataggtttc cctatatgct gactatttta gctactaact catgactaaa gtcacaagtg
      241 ttcgtagcta ttttttaatc aaactggaat acagactcca ttcctaggaa attggctgaa
      301 atatagtctg ctgtcaaaga tgctgtcatc acagaaatta aaacagatac tgaaaagttt
      361 ttatggactt cttcaagtga aaacataaca cccgctagtg gtgcatgaaa ggctgctgat
      421 aatccagcac ttgccccaca ggttaacagg aatttttcct ctgtcttccc tcggtcaaga
      481 gctctagaaa tacctttacc tgccatagca ccaagctgaa tggaaggacc ttcccttccc
      541 aaagcaagac cacccataag tgataagaat cctccaataa attttgttgg caacactcgc
      601 caccatactt gttttatttt ccctgtcatt tccccctcaa gctgagggat accacttcca
      661 gaaatcattg gctcccagtc caccaacttt gatacaatcc acgcaagtac tgcaagtact
      721 gcaaaccaag ctattatttt tattgtagaa ccttctacaa attttaaaat ttgactaagc
      781 caagtaccag cctgccctaa aagtatcctg tataaaagca caatcaaacc agccacagcc
      841 ccaaccataa gtccttcacc aatcaaaatt acctgaaagt gctctgcccg tttgataata
      901 tgagatgtat cctttttcat actgttcctc cttcatccaa taattctaca caatctgaat
      961 ctccaatgac taaagtcatg agtgtgtaca gatatttttt aatcaacaaa aatagatttt
     1021 tatttactaa atataagaaa ataattgctc aatttctcaa taaatgtgaa tttattcttt
     1081 taggtattta ttctccacag tcgtgcttct tccagcaaaa tatttattag ttcttgcttt
     1141 ctctgtgtat agattttacg attgtcctca aactgtaatg ctaatttttg tttatgacta
     1201 tcatagagtt ttgctttgtc tgtaaaagta tttagataat ctcgaaaatt gatataatta
     1261 ttccatgcaa cactattcca ttcaacaaca tgaatatggt gagtacgagt atttttctca
     1321 aaatctccca tagcaaaaag taattgtttt ggtacatcct gacctcgaaa aacgattcca
     1381 cattgctcta acctttcgag gtataattta gcttcctcta aagaagcaac acctacaaca
     1441 atatcaataa taggctttgc gctaatactg ggaatagaag tgcttccaat gtgttgaata
     1501 tcaatagcag tcttatccca tatacttttc aataatataa tgacatcttt tgcactttta
     1561 tcccatatca tctgatgttc caataattta accataccac attttaaacc tatcactata
     1621 taccacctca atataatcca cctttatata tattacaatt ttttctatct tgataaacag
     1681 tgatttagct atttcacttt ttcttgacac tcgctacatt ccctatcatg aatagatata
     1741 attcctccac atttagaaca agtatatctc tccttctgct tttgcataaa cttctctaaa
     1801 ccgtactgct gaacaaattt actattttcc ataaggcttg cttgatatct cttgttataa
     1861 cttttttcaa gattttttat gagtttacaa ggaaaattga aacattcaaa acaataaaac
     1921 aacccttttt gacagacaca atcctttatt ttacacttac ggcagtgttc tggctttcct
     1981 gtattgccgt tcaaacaacc agcacatggc tttttgtgat aacaatgctt atagcaaacc
     2041 atacaattca ttccacaagg agcaaacata atcgtatcaa tactctcttt tggcattttc
     2101 ataacatttt actcctttca aaaatccaat ttgtcgaatt gttatatatt catattgtaa
     2161 cataaaaaat agctaaaaaa ttcatgcact ttcattgcca ataaattttc aaggaaatat
     2221 gaaggtttca aactaagaat aaaaagagat aaaaaggaag ctactaatca aaatattcaa
     2281 acacaagtaa taaatataaa taacttaagg tatatttaaa agtatatgat attaccagca
     2341 gttttaaata caaatataac cacacatgcc tcaaaaacac ttattacagt atcttagaaa
     2401 cttagacaat acataagatt cattaataaa aaagttctca atatagatat atttaaccat
     2461 gttttgaata gcagataatc atatgattaa aaattaaata tgacatagcg tttgtggaat
     2521 ataacaccta aattatgtta taataataaa gcaggagtgt gatagtgatg gatgaaaaaa
     2581 tattagagtt attacaggaa atgcaaggta atattagtaa tatgcaaggt agtatcagtg
     2641 gtatcgaagg cactattagt gatatgcaaa gcaatattaa tggtatcgaa gacactatta
     2701 gtggcatgca aggcactatt agcgatatgc aaggcagtat cagtggtata gatgacacta
     2761 ttagcgatat gcaaggcagt atcagtggta tgaatggcac tattagcgat atgcaaggca
     2821 gtatcagtag tatggatggc actattaata gtatgcaaga cagtattact gatatgcaag
     2881 ttgaaattaa agaagtcaat agtaggttag acaatatgga acctgatatt aaagacttaa
     2941 aaactggaca agttgaaact agtagtagac ttgatactat tcagagtgat gttacagaaa
     3001 ttaagtcaga tgtaaaaatt aacagaaata cattagatac agtgattaaa catacctctt
     3061 cattagtaaa agatacatca gatattaaaa atgatttaaa taatatagag atagtcacat
     3121 ctaagaattc gaatgatatt gctaaattaa aatcagttaa ataatatact atagtaaaaa
     3181 atacctgaaa atttcacata cttctttaaa gttataccaa attttataca gaaatactga
     3241 ttatccgaca acacacttta cttataaata atttattaaa aaaatactca ttttatccta
     3301 aaattaacac gactaaatga gtcttgatat aataataaaa aataagggag attagacact
     3361 aaaaagttgt gtatctccct tatattctat aatatagtta tataattttt cagcctaaaa
     3421 taaaagtcca tctttgatta aaacacatcc ccttatcact tataaaagga taaaatcttg
     3481 ttttcatttt tccatttata tcttcttgct taagagtaat aattttttct tttgatactt
     3541 taagtgtttg gttaaattta accaatcgac taagttttgt tcaacatcta tttttcatat
     3601 tttccaatac ttaatctatt ttttataaat aattctttca tttctaactc acttacattt
     3661 tttacataaa acgaaacata taaatttcaa ccttatcttg tatggttctt aatacttaat
     3721 gcgtagctta agtatcaaac attataaatt ttccccttac atttaacact cttaccattt
     3781 ccaaaatata caaaatatat tttgaacatt tgtacaatga tgaaatacaa tacatgaaaa
     3841 tacaatatca taactgttgt ctaaaaatag aagttcttca acataacctt tcacaaaaaa
     3901 catattatct aagtattttt tgtctgctcc ttgttttcct gtttgtagca tataaattac
     3961 tgcatctaaa catataacta ctcacacgaa aaaagaaaat aaacttccaa aatacaagct
     4021 ctaaaaacta cctctaaagt tgtataatgt ttactaggaa taaggttgta taacatatag
     4081 tttaaataat cattttttaa aattgtaagt tattgctttc aaaattagat gtttgtatat
     4141 caaataaatg attaagaatt tctaaattta atggttttat aatgcactcc ctttctttgg
     4201 gataaaataa tgttgcacag atactttttc taaagataac aattttattt ttctatcaat
     4261 tccaccagca tacccagtca aactcccatt tgttcctaca acacgatgac atggaataat
     4321 aatagagatt ccattatgcc ctactgcacc accaattgct tgtgctgaca ttttagatgt
     4381 tcctttttgt tgagcaactt tatatgcaat ttctccataa gtgattgttt taccatatgg
     4441 tatttgttgt aaaattttcc acactgtagt ctgaaaagga gtacctacca tatggagtga
     4501 tggtgtaaaa ttgggttctt ttcccgaaaa ataaatatct aaccatttct ttgtctgctc
     4561 gaaaattggc aattcttttt cttgatgttc ttggtcaaga ttataagcat aatacttttg
     4621 attctcaaac caaagaccag ttaacccaat attatctgat gctaaaagaa tatttccaat
     4681 tggactatgg taatagctaa tatattgcat acaattcctc cttatattct gaaaacttca
     4741 ctcaaatcat tagacacttc ttttctatct gccattatgt aaaaacatac taatataatt
     4801 tccttctaac tacactttcg ttataatatc tatcctcata tcatattagt tttatattta
     4861 cctgcatctg ttcatccttt tttccctttc cctttgatga atataaaatt catacattta
     4921 agcttcctac tgtattaact tttatagaag tgtatattat aattttatca ccaaatagac
     4981 caaaaaaata tcgatatagc atcaagtaat atcataatac tttcaaatgg gaaaagtaag
     5041 aataggctgt aaaaaagaaa agtattttta tgttagaaat ccagtaaaat cgttagtaga
     5101 tagaataaca gaaaacgaac aatttgctat ttatcatttt acacataaat ataattggcg
     5161 caataaagtg ttttctattc tacaacgatt aataaggtta gaaaaaagta aaaatgaact
     5221 gtatatgtat gaatcattag tttctgtttc tttcctttgg cttattatga taaaaaatat
     5281 tgttttgcca ccaataaaac acaaaaatat aattaatatc catatgcaaa aaattttacg
     5341 ttatattgaa aaatactatt gttttatatt atattgaaga gtactattgt gaaaacttaa
     5401 ttttggagta ctatcaagca gtgataatat caataaatca gagtgttcca gaggatttga
     5461 actaagttta aatactatac cttgtaaata ttttactgag ttttgtttat taaaagcaac
     5521 tcaatttcta aaaggcacaa atgagctaat aagtgacatt actataaaag ttgtttttca
     5581 tcaaataatc tattttagta aatgttttaa agaaaaatgg gatattctcc aaaagtgtac
     5641 agaaatataa aaaaagagat atgatttttt atttcttttg attatccaaa gagtaaataa
     5701 aaaatattgt aaaaaactta ctaacaacgg caatgctttg aactatggta atactttgaa
     5761 cttttgattg gacttttttt gctaaaataa tatatttaaa tagcaaagta atctgtttaa
     5821 tcaataaaaa ataagagata aaagaaatca actaaaactt ttacctctta ttttgatagt
     5881 ttatcagagc taatatttac actcttttta tttttgatac ttcattaaaa atgatgctta
     5941 ctctcca
//
LOCUS       DBGJFF010000185         5832 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.190, whole genome shotgun sequence.
ACCESSION   DBGJFF010000185 DBGJFF010000000
VERSION     DBGJFF010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5832
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.190"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(102..1835)
                     /locus_tag="ACOIAF_003581"
     CDS             complement(102..1835)
                     /locus_tag="ACOIAF_003581"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HGZ2670561.1"
                     /translation="MSILLKKAPKLAKHIITSFYINRDINEVLKYLCENVTWIGPGEQ
                     EFLTSFNEIKNYFYAGQDEIPSCDINNDIFETVSEDENRCMVLGRYTVRTKENAQMIL
                     EVNQRCTFEIIEDRGKLLIKHMHISNPYGEMQLDEYFPTKIGTQSYDYLQRLLKEKTE
                     VIEMITNNINGGLKGSNDDSTYSFFYVNEGLPKILGYTYDEFMEMSGGSAVGAVYPPD
                     LPKALEDCERCFAKGPTYSSEYRIRKKDGTLMWVLDSGMKSLNSDGIVKINSIITDIT
                     QLKNIESELKLERERYRIALQNITDIMFEYDIENDTLIKYQRVEIDKKIELENFETKN
                     YSKLLESGKIIHLDDIGKLLEVLHGNLRETIEIRQVNSLTKNEWRWIRVQCSVIYDSD
                     HNPIKTIGVLKDITEDKSKLESLINQAQRDPLTQLYNQRVSQNLIEEYLCSSDSKNND
                     ALLVIDIDDFKTVNDTFGHLEGNEVLVAVSKILLHNTHDKDIVARIGGDEFTIFIKSL
                     TKDLIIKITNDILNDASKIKVKDNHKITLSIGIAFTDDSTKLYKDLFSKADKALYLSK
                     ADGKNCYSTYE"
     gene            complement(2075..2920)
                     /gene="dccA"
                     /locus_tag="ACOIAF_003582"
     CDS             complement(2075..2920)
                     /gene="dccA"
                     /locus_tag="ACOIAF_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP diguanylate cyclase DccA"
                     /protein_id="HGZ2670562.1"
                     /translation="MFKEIFLRTFPGFLIIRDSNYRIIFINDNLKNLIKSYMQDNPLG
                     MTNIEIAKKLPDNIAKFFTDSHNIQLDWEKNYPYDKISNWILEFKKDTTSYWNVLEYK
                     VDVDEKTYIITMANDITKLYEENKRNLHYSITDPLTGAYNRKYLNDRFDIFIGDYIVL
                     IDLDNFKMINDYEGHNVGDKILCDFVSLLNKELINSTSIIRLGGDEFIVIFSSDVDKN
                     YVYSQLEALRENFLKVFSKYKYLSFSYGVDTVKRNLKLTIVELDKKMYKNKEKNKKKF
                     DKNDY"
     gene            complement(2951..5185)
                     /locus_tag="ACOIAF_003583"
     CDS             complement(2951..5185)
                     /locus_tag="ACOIAF_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HGZ2670563.1"
                     /translation="MQNHNYIKMLNKKVEVLSKELDSRNFKLKTLNEIDFFSKIPNRE
                     FMYQYIDDCISKNIHIGIMLLNIDNFKLFNDIYGYLVGENLLITLTQNLLHFIPESKG
                     IVAYLKGEGFLIVLPNVSNKELNNMGATIIESARELKIKLPNEKEIYSNVTFSIGSTI
                     WNGEPNMTTLMLFNQVYSALKKAKSEGKNRYILFDFKDNSFYNKLENDKEFLSEISHV
                     LQTKQIEVFYQPLYNIKNNTIVGCEALARWRHPIKGLLTPDKFIPLFEQFGLITELDL
                     HLFEENCKNIRRWIDNNDVFVPICCNFSRLHFFNPNFPSMLKGITEKYSVSTSNFSIE
                     ITENILIENTNTIIQQLAELRSYGFSVSMDDFGSGYSSFGMLQNLPFDIIKIDRIFLS
                     HNLNDFKNTTILYSIINIARALGMLTVCEGVETEQHVEFIKSINCDIAQGYYYAKPME
                     CKEFEKLLSKENNSEKMFIKYSDIGKSLIEKIFDGFFIMQDMKKLRANVTEDIQWFDF
                     YNKTEITSFPKVIEHFEKHIKGKKFSILYKSITPHNTESDSLLISGEAVLSDEASEHQ
                     KYSNFYFSANCIISENKLLLAKLHLDLIKTAGYINEFESYSIPKMTNTHLDESNILSQ
                     FYSMLPVGIIRYDLFGDMIITYMNEEMFDIIGYTKEQFFGEMGGNLRAIVHPDDLDFV
                     YKKSLDMIDTNKIEPFLYRFIKRDGQIVTVMYTQCNLSAIDGRSIAQGMYVDVNKIKN
                     LTKI"
BASE COUNT         1995 a          815 c          652 g         2370 t
ORIGIN      
        1 taggaactag ttgactagct ataagattag cttcaactct ttgattgtta gactatgaaa
       61 ttaaatttaa aggttcattc ttggtcgtat aaatagcttt attattcgta tgtactataa
      121 caatttttgc catctgcttt tgatagataa agagctttat cagctttaga aaataaatct
      181 ttatataatt tagttgaatc atcagtgaag gcaataccaa tacttaatgt tattttatga
      241 ttgtccttta cttttatttt actcgcatca tttaaaatat cattagtaat ttttattatt
      301 aaatcctttg ttaatgattt tataaatatt gtaaattcat ctccaccaat tcgagcaact
      361 atgtctttat catgtgtgtt atgtaatagt atttttgata cagcaactaa aacttcattt
      421 ccctctagat gcccaaaagt atcatttaca gttttaaaat catcaatatc aataactaat
      481 agagcatcat tatttttgga atcagaacta catagatact cttcaataag attttgtgat
      541 actctttgat tatataattg tgttagtggg tcacgttgag cctgattaat taaactttct
      601 aatttagatt tatcttcagt tatgtctttt aaaacgccta ttgttttgat agggttatgg
      661 tcactatcat atataactga acattgaaca cgaatccatc tccattcatt tttagttaat
      721 gaatttactt gtcttatctc aattgtttca cgaagatttc catgaagtac ttctagtagt
      781 tttccaatat catcaaggtg aataatttta ccagattcta atagtttgga gtaatttttt
      841 gtttcaaaat tttctaattc aatttttttg tcaatttcta cacgttgata ttttataagg
      901 gtatcgtttt caatatcata ctcaaacatg atatctgtaa tattttgcaa agcaatacga
      961 tatcgctctc tttctaattt tagttcagat tctatgtttt ttagttgggt tatgtcagta
     1021 ataatactat taatttttac tatgccatca ctatttaacg atttcattcc agaatctaat
     1081 acccacatta atgttccatc tttttttcgg attctatatt cagatgaata tgtaggtcct
     1141 tttgcaaaac atcgttcaca atcttctagt gctttaggta agtctggtgg atatacagca
     1201 cccactgctg aaccaccact catttccatg aactcatcat atgtgtatcc taatattttg
     1261 ggcaatccct cattgacata gaaaaatgag tatgtactat catcattgct accttttaag
     1321 ccaccattga tattatttgt aatcatttca ataacttctg ttttttcttt tagtaatctt
     1381 tgcaaataat cataactttg agttccaatt ttagtgggaa agtattcatc aagttgcatt
     1441 tcaccataag ggtttgatat gtgcatgtgc tttattaata attttcctct atcctcaatt
     1501 atctcaaatg tacaacgttg atttacctct aaaatcattt gagcattttc tttagttcga
     1561 acagtatatc ttcctaatac catacatcta ttctcatctt cacttacagt ttcaaatata
     1621 tcattattta tatcacaaga aggaatttca tcttgacctg cataaaaata attttttatt
     1681 tcattaaaag aagtgagaaa ttcctgttct cctggtccaa tccatgtaac attttcacaa
     1741 aggtatttta atacttcatt gatatctcga tttatataaa aacttgttat aatatgtttt
     1801 gctaatttgg gtgctttttt taggagtatg ctcatgatat tcctcctttc tttttgctat
     1861 tatattttaa ttatattata catcaatata tttaaattaa aagctttgtt aaactatgat
     1921 atctttgtta aataagttaa ttttaacaaa aaagaactga aacaatatat gcttcagtac
     1981 ttaatgtcaa tatcttgtat agatacatat ttataaacta atgtacaagt ttaaaaaaag
     2041 aaagataatt ttaatttatt ttaaaataac aatgttaata atcattttta tcaaattttt
     2101 tcttgttttt ctccttgttt ttatacattt ttttatccaa ttcaactatt gtcaatttta
     2161 aatttctttt tactgtatca acaccataac taaatgaaag atacttatac ttagagaata
     2221 cttttaaaaa attttctctt aaggcttcta gctgtgaata aacataattt ttgtcaacat
     2281 cactagagaa tataacaata aattcatctc ctcctaatct tataatagaa gtagaattta
     2341 ttaattcttt atttaaaagt gaaacaaaat cacataaaat cttgtctcct acattatgac
     2401 cttcatagtc atttatcatt ttgaaattat ctaagtcaat tagtacaata taatctccta
     2461 taaatatgtc aaatctatca tttaaatatt ttctattata tgctccagtt aatgggtcag
     2521 taattgagta gtgaagattt ctcttatttt cttcatagag ttttgttata tcattggcca
     2581 tagtaattat gtaggttttt tcatcaacat ctactttgta ttctaaaaca ttccaatagc
     2641 ttgtagtatc ttttttaaac tctaaaatcc aatttgaaat tttatcataa ggataatttt
     2701 tttcccaatc taattgaata ttgtgagaat ccgtaaaaaa tttagcaata ttatcaggga
     2761 gtttcttagc tatttctata ttagtcattc caagtggatt atcttgcata taacttttaa
     2821 ttaaattttt taaattatca tttataaata taattctgta gttagaatct cttataatta
     2881 gaaatcctgg aaaagttctt aaaaaaattt ctttaaacat attaatttct cccaaataaa
     2941 atactttgta ctagattttt gttaaatttt ttattttatt tacatctaca tacataccct
     3001 gggcaatcga ccttccatca attgcagaaa ggttacattg tgtatacata actgtgacaa
     3061 tttgaccatc tcgttttatg aatctatata aaaatggttc aattttattt gtgtcaatca
     3121 tgtccaaact ttttttataa acaaagtcta aatcatctgg atgaacaatt gctcttaaat
     3181 ttccgcccat ctcaccaaaa aactgttctt ttgtataacc tataatatca aacatttctt
     3241 cattcatata agtaataatc atatcaccaa ataaatcgta gcgaattata cctacaggca
     3301 acattgaata aaattgtgat aaaatattac tttcatcaag atgtgtattt gtcattttag
     3361 gtattgaata tgattcaaat tcatttatat agccagcagt tttaattaaa tccaaatgta
     3421 attttgctaa gagtaattta ttttctgaaa ttatacaatt tgcactaaag tagaaattgc
     3481 tatatttttg atgttcagaa gcttcatctg ataaaacggc ttctccacta attaataatg
     3541 aatctgattc agtattatgt ggggttatac ttttgtaaag aatagaaaat tttttacctt
     3601 taatatgttt ttcaaaatgc tctattactt taggaaaaga tgtaatttca gttttgttgt
     3661 aaaaatcaaa ccattgaatg tcttcagtga cattagctct tagttttttc atatcttgca
     3721 taataaaaaa tccatcaaat atcttctcta ttaaagattt tcctatatca gaatatttta
     3781 taaacatttt ctctgaatta ttctctttag atagtaattt ttcaaattct ttgcactcca
     3841 tgggttttgc ataataataa ccttgggcga tatcacaatt tatactttta ataaattcta
     3901 catgttgttc tgtttcaaca ccttcacata ctgtaagcat cccaagagct cttgcaatgt
     3961 ttattataga gtacaaaatg gttgtatttt taaaatcatt taaattatga gataaaaata
     4021 tcctatcaat tttgattata tcaaaaggta gattttgcaa cataccaaaa gatgaatatc
     4081 cagaaccaaa atcgtccatt gatacagaaa aaccataaga acgaagttca gctagttgtt
     4141 gaataattgt atttgtattt tctattaata tattttctgt tatttcaatg ctaaaatttg
     4201 atgtagagac gctgtacttt tcagtaatac cttttagcat acttggaaaa ttagggttaa
     4261 aaaaatgaag acgagaaaag ttacaacaaa ttggaacaaa aacatcattg ttatcaatcc
     4321 atcttcttat atttttacag ttctcttcaa ataaatgcaa atctaattcc gttatcagac
     4381 caaactgttc aaataaagga ataaatttat ctggagtaag aagtccttta atagggtgtc
     4441 tccatcttgc aagtgcctca catccaacta tagtattatt ttttatatta taaagtggtt
     4501 ggtaaaatac ttcaatttgt ttagtttgta gaacatgtga tatttctgat aagaactcct
     4561 tatcattttc taatttattg taaaaactat tatctttaaa atcaaataga atgtatctat
     4621 ttttaccttc acttttagcc ttttttaaag cactatatac ttggttgaaa agcatcaaag
     4681 tagtcatgtt tggttcacca ttccagatag tgctgccaat actaaatgta acattggagt
     4741 atatttcttt ttcattagga agttttattt ttaattctcg ggcgctctct ataatagttg
     4801 cacccatatt atttaactct ttatttgata catttggaag tacaattaaa aaaccttctc
     4861 cttttaaata agcgactatt ccctttgatt ctggaataaa gtgcaaaagg ttttgagtta
     4921 gagtaattaa taaattttct ccgactagat atccatatat atcattaaat aatttaaagt
     4981 tatcgatatt taataacatg attcctatat gtatattttt cgaaatacag tcatcaatat
     5041 attggtacat aaattctctg tttggtattt tagaaaaaaa atctatttca tttaaggttt
     5101 ttaatttaaa atttctacta tctaattcct tagataatac ttctaccttt ttatttaaca
     5161 tttttatata attatgattt tgcatggtat tcctccataa gatactttaa attttgatga
     5221 caactgtatt tatgctagaa gtatttaaat gttaatttgt aaataattta aatgctaaat
     5281 tttaaataag tatttaaaat gaatatacat taagttactt gctccaatgc tacaaataca
     5341 aatatttttt agtttagtaa taatattaga taaaattagt taatgcttaa attctagtat
     5401 attgaaagat ttcctttttg aatgcaatta tatcatattt tttcataaat agatagattg
     5461 tgagctgtat agttagtaag gtagagtttt tttataatta attttaagtt taatgatttt
     5521 tcaaaggcat aattatttta ttcataatta actttttata cataatttaa taccatatta
     5581 ataattttgc ataattaatt ttagttatta aaattaagtt tattcttgta ttttttgtta
     5641 aagactataa taaatattca tttactaaat ttataaacta ttaatctata gaaatcagtt
     5701 ttaatatgaa aaatattaca tagagatgga tataatagct gtaaacataa aactatataa
     5761 aactaaaata tatttataaa aaagttgaag ttataaatac tatacctaat attacaatat
     5821 aaaaatattt ta
//
LOCUS       DBGJFF010000186         5831 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.191, whole genome shotgun sequence.
ACCESSION   DBGJFF010000186 DBGJFF010000000
VERSION     DBGJFF010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5831
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.191"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            428..1189
                     /locus_tag="ACOIAF_003584"
     CDS             428..1189
                     /locus_tag="ACOIAF_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896901.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HGZ2670564.1"
                     /translation="MIGIYFSGTGNSRYCVEKFLQEYDITANSYSIEDNELLQHINNH
                     ENIIFSYPVQYSNVPKILKDFIINHSTLWNGKRVFIIATMALFSGDGSGVLARILKKY
                     GAITVGGLHVKMPDSIGDEKVLKHSLEYNRKLVIKAEKKVTKAAKKLKHGSPPKEGLV
                     FLSHIVGLFGQRLYFINKTNMYTDKLKINTEECIVCGKCVNLCPMKNLVIKNCMIVAN
                     GQCTMCYRCVNNCAKKAITLLGKKVVEQSNIKKYM"
     gene            1254..2036
                     /locus_tag="ACOIAF_003585"
     CDS             1254..2036
                     /locus_tag="ACOIAF_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HGZ2670565.1"
                     /translation="MEYYIVDSFATKLFKGNPAGVCVLDRRIPLELMQKIAEENNLPE
                     TAFVVKGKGNYELRWFTPKAEIDLCGHATLAAAYVISNFIDVNVKKIDFFTQSGKLEV
                     TRNGNLYEMIFPEIMPIEIELSPQQANLIGCVPSDVYSSRDLILLLNSEQEVINYKPN
                     YAQLRKLTDWLGIIITAQGSNTDFVSRYFCPELDSEDPVTGSSHCNLIPYWSEKLGKH
                     KMVAAQLSNRGGIIQCEVLKDNTVKISGEAVLFMQGTIKIDI"
     gene            2303..2722
                     /locus_tag="ACOIAF_003586"
     CDS             2303..2722
                     /locus_tag="ACOIAF_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjdF family protein"
                     /protein_id="HGZ2670566.1"
                     /translation="MDKISGKLTVLFEEPFWVGIFERQVGKKYEACRVVFGAEPKEVE
                     VYEFILERFFSLDFGSIKLEKNVTKDNIGYKRMQRKVKKEQEKETIGTKAQNALKLQY
                     EERKQDRKNLAKNRKEEEKERLFNLKQEKRKAKHKGH"
     gene            complement(2825..3091)
                     /locus_tag="ACOIAF_003587"
     CDS             complement(2825..3091)
                     /locus_tag="ACOIAF_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HGZ2670567.1"
                     /translation="MKNFILIYFIVINSIAFFSMYIDKKRAIRNEWRIKEATLMSIAV
                     IGGSIGSMIGMYSFRHKTKHIKFTFGIPFILFLQFLLVYFYILK"
     gene            3303..4004
                     /locus_tag="ACOIAF_003588"
     CDS             3303..4004
                     /locus_tag="ACOIAF_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HGZ2670568.1"
                     /translation="MKFIVEKEVFDKLENVCFGVVVAKGIDNTKEIERISNLLDISID
                     RVEDYFKDKKVKESEEIIPYREAFRSLGMNPNKFMSSIEAMTTRVAKNKKLPHINPIV
                     DLGNSISLKYLLPMGAHDMDFRNDDVYVRFSKKGDKFVPFGETDVELMEEGELIYSVG
                     DMVKTRRWIWRQGEEGKITNSSKNIFFPIDGFTDANLDKVMSAREELAKLLKEIFNCE
                     IKVGFVDKDNPEMEI"
     gene            4086..4694
                     /locus_tag="ACOIAF_003589"
     CDS             4086..4694
                     /locus_tag="ACOIAF_003589"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HGZ2670569.1"
                     /translation="MKKTIIKNVNPMIENKFIGLFEIEYKNKLDEDKVWMVASRKSSE
                     QLKNIYLKNEEDSVDAVVIVGLHKSSKKLILIRQFRVPINGYIYELPAGLVDEGESID
                     ISVERELREETGLTLLEIKKNKSIDKVYLSPGMSDESVAFVYCICDGDITDEFLEPDE
                     EIEALLISQEDAKEILQSNHKIDTKAFLILQMFVSLGAKLFE"
     gene            5018..5482
                     /locus_tag="ACOIAF_003590"
     CDS             5018..5482
                     /locus_tag="ACOIAF_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4624 family lipoprotein"
                     /protein_id="HGZ2670570.1"
                     /translation="MKKLVIILISIISMVSLTACKKSNESKYNNLNNKESGQTTIEMD
                     LDKNYDTSDPFVNARLFCVSNDIDVLDTEISFKMDGDSGIVEIKDNKTDETLWSNTWH
                     GSVDNDTFTISLSNIQKEKEYAICFTGIKINHAVVKVTFESNLVKEKEKPSK"
BASE COUNT         2381 a          533 c          995 g         1922 t
ORIGIN      
        1 ttatacatat taggcaatag ggaatttttt tatagttaaa ttttcaaata ttttttaggg
       61 aactttataa ttgagaatta agggtatagt atgtgatgaa cttactaaat ttataattag
      121 gttatataat ggaataatca tcaatagttt tactttaggg aaaatatatg cttaaggaag
      181 tatagcttac taatattatt aaaatgtata tttaaaatga taaaaaaaga attagacaag
      241 tatttagtaa attgactatg caaaattcta tcaagcagga acactatgac tttagtcatg
      301 ggaggttcag taggtaactt gttgaattaa gtttatatta tttaaataaa ctacttaatt
      361 tagataatat aaaaagtcag aattttaaga atagagctat ataatcttag ctattaggag
      421 aagtactatg ataggaatat attttagtgg aacaggaaat tcaagatact gtgtagaaaa
      481 atttttacaa gaatatgata taacagcaaa ttcttattct attgaagata atgaactttt
      541 acagcacatc aataatcatg agaatattat ttttagttat cctgtgcaat atagtaatgt
      601 gccaaaaata ttgaaagatt ttataataaa tcattcaact ttgtggaatg gtaaacgtgt
      661 ttttattatt gcaactatgg ctctatttag tggagatggc tctggtgtgt tagctagaat
      721 attaaaaaaa tacggtgcta taactgttgg tggattacat gtgaagatgc cagatagtat
      781 aggagatgaa aaggtattaa aacattcttt agagtataat agaaagcttg taataaaagc
      841 agaaaagaaa gtaactaaag ctgctaaaaa actgaaacat gggtcaccac caaaagaagg
      901 gttggttttt ttgagtcata ttgtagggtt atttggacaa cgattgtatt ttatcaataa
      961 aacaaatatg tatacagata aactaaaaat taatactgag gaatgtattg tatgtggtaa
     1021 atgtgttaat ttatgtccaa tgaagaatct agtaattaaa aattgtatga ttgttgcaaa
     1081 tgggcaatgt acaatgtgtt atcgttgtgt aaataattgt gccaaaaaag caattacatt
     1141 gttaggtaaa aaagttgttg aacagagtaa tattaaaaaa tatatgtaag aaatttaaag
     1201 ttttattgaa tgtaaattga tgtatatttt atgttagaaa gagaggttaa ataatggaat
     1261 actacatagt tgattcattt gcaactaaac tatttaaagg aaatcctgct ggagtttgtg
     1321 ttcttgacag aagaatacct ttagagttaa tgcagaaaat agcagaagaa aacaatttac
     1381 cagaaacagc atttgtagtt aaaggtaaag gaaattatga attacgatgg tttactccta
     1441 aagcagaaat tgatttatgt ggtcatgcaa ctttagcagc agcttatgta atatcaaatt
     1501 ttattgatgt taatgtaaaa aagatagatt tctttactca aagtggaaag cttgaagtta
     1561 cacgaaatgg aaatctatat gaaatgatat ttccagaaat aatgcctatt gaaatagagt
     1621 tatcaccgca acaagctaat ttgataggct gtgtaccaag tgatgtttat tcttcaagag
     1681 atttaatctt attgctaaac agtgaacaag aagttataaa ttataaacct aattacgcac
     1741 aattgcgtaa attgacagac tggttaggta taataatcac agcacaagga agtaatacag
     1801 attttgtatc aagatatttt tgtccagaat tagattcaga agaccctgtt actggttcat
     1861 cacattgtaa tcttattcca tactggtctg aaaagttggg aaaacacaaa atggtagctg
     1921 ctcaactttc taatcgtggt ggtataattc agtgtgaagt gttgaaggat aatactgtaa
     1981 aaatttctgg tgaagcagtc ttgtttatgc agggaacaat aaagattgat atttaaataa
     2041 gtataataac taatttatta tgaaaaataa ttaattttct tagtgttaac aataataaag
     2101 tagagttaaa aaacttgatt tcacttaaat caatatgata gatgttgata ctaagattga
     2161 caagcatgat taaaaaagat aatatttaat ctataaagat aaaaacacag tctggttggt
     2221 agtccagacg cagcaatagc tgtcagtaac ctgcctcctt ggttgtccgt tctttagaaa
     2281 gtaatttaag gaggaactag caatggacaa aattagtgga aaattaacag tcttatttga
     2341 agaaccattt tgggtaggta tatttgaaag acaagttggt aaaaaatatg aagcatgtag
     2401 ggtagttttt ggagcagaac ctaaagaagt agaagtgtat gaatttatac ttgaaagatt
     2461 tttctcatta gattttggta gcattaaatt agaaaaaaat gtgaccaaag acaacattgg
     2521 atacaaaaga atgcagagaa aagtaaaaaa ggaacaagaa aaagaaacta tagggactaa
     2581 ggctcaaaat gctcttaagc ttcaatatga agaaagaaaa caggatagaa aaaacctagc
     2641 taaaaataga aaagaagaag aaaaagaaag actatttaat ttgaaacaag aaaagagaaa
     2701 agctaaacat aagggtcatt aattttaatt aaatatcttt ttaataattg ctagaaaaag
     2761 gtgtataaaa tttttaattt tatacacctt aatttattga aaagaataag gttttatatt
     2821 taaattactt taaaatataa aaatatacta ataaaaattg taaaaatagt ataaatggta
     2881 taccaaatgt aaatttaatg tgtttagttt tatgtctaaa agaatacatt ccaatcatag
     2941 aacctatact acctccaatt actgctatag acataagagt agcttctttt attctccatt
     3001 cgtttctaat tgctcttttc ttatctatgt acattgaaaa gaaagcaata gaattaatca
     3061 ctataaaata tattagaata aaatttttca cagtcttgcc tccataaaaa tatattatac
     3121 attataaaac aagatatttg ttttggaaag ttaaatttgt aattagtaca tataaaatat
     3181 ttgatattaa ttggaaaatt taaaaaaatt aaacaattag caaatagggg gtatatttat
     3241 aagacacaaa gtgataaaat ctatttagaa aataatgtaa aataacagga ggataaagca
     3301 aaatgaaatt tatagtagaa aaagaagttt ttgataaatt agaaaatgta tgctttggtg
     3361 tagtagttgc aaaaggaata gataatacta aagaaataga aagaataagc aaccttttag
     3421 atataagtat agatagagta gaagattact tcaaagataa aaaagtaaag gaatcagaag
     3481 aaattatacc atatagagag gcatttagaa gtctaggcat gaacccaaat aaatttatga
     3541 gttcgataga agcaatgaca acaagggttg ctaagaataa gaaacttcct catataaatc
     3601 ctattgtaga tttaggaaac tctatttctt taaaatattt acttccaatg ggtgctcatg
     3661 atatggattt tagaaatgat gatgtatatg ttagattttc taaaaaaggg gataaatttg
     3721 taccatttgg agaaactgat gttgaattaa tggaagaagg agaattaatt tattcagttg
     3781 gagacatggt aaaaactaga agatggatat ggagacaagg agaagaagga aaaattacta
     3841 atagctcaaa aaatatattt tttcctatag atggttttac tgatgctaat ttagataagg
     3901 taatgagtgc aagagaagaa ttagcaaaat tgttaaagga aatatttaat tgtgaaatta
     3961 aagtaggttt tgtagataaa gataatcctg aaatggaaat ataataagtt gttgaatact
     4021 tgttaagata ttatatttac ttaatgatgt taaaaaggta ttaaaaaaga aaggtcgtag
     4081 taaatttgaa aaaaactata ataaagaatg taaatccaat gattgaaaat aagtttatag
     4141 gactttttga aatagagtat aaaaacaaat tagatgaaga taaggtttgg atggtagcat
     4201 caagaaaaag tagtgagcaa ttaaaaaata tttatttgaa gaatgaagaa gatagtgtag
     4261 atgcagttgt aatagttggg cttcataaaa gtagtaaaaa attaatttta ataagacaat
     4321 ttagagtacc tattaatgga tatatatacg agttaccagc aggtcttgtt gatgaaggag
     4381 agtctataga tatttccgtt gaaagagaac ttagagaaga aacaggactt actcttttag
     4441 aaattaaaaa aaataaaagt attgataaag tttatttatc tcctggaatg agtgatgaat
     4501 ctgttgcctt tgtttattgt atatgtgatg gtgatataac agatgaattt ttagaaccag
     4561 atgaagagat tgaagcgctt ttaatttcgc aagaagatgc aaaagagatt ttacaaagta
     4621 atcataagat agacacaaaa gcatttttga ttttacaaat gtttgtgagt ttaggagcta
     4681 aattatttga atagatatat agaaaagagg ttatatagaa gagtaaaata ataaagttta
     4741 ttttacttgt gtaaattata atctcttttt ttatacaata aagtataatt ttgtgtattt
     4801 ttaaataagt gatttgactc ttaaattttc ataatatgtt ataaaccaat ttaagatact
     4861 acaataatta aaattccaaa attatttaac atatttgcaa ttaaaatgag aaaatgtaaa
     4921 ggctgaatga ttgaaaaatt tgatgtaaaa tagtagctta gtttaatact atagtttgta
     4981 gggttaaaaa ttagaaattg taagggagat aaattctatg aaaaaattag ttattatttt
     5041 aattagtatc attagtatgg ttagcttgac agcttgtaaa aaaagcaatg aaagtaaata
     5101 taataattta aacaacaaag aatccggaca aacaaccatt gaaatggact tagataaaaa
     5161 ttatgatact tcagacccat ttgttaatgc acgtttgttt tgtgtgtcta atgatataga
     5221 tgttttagac acagagattt cttttaaaat ggatggagat agtggtattg tggaaataaa
     5281 ggacaataaa acagatgaaa ctttatggag taatacatgg catggaagcg ttgataatga
     5341 tacattcaca atttctcttt ctaatataca aaaagagaaa gagtatgcta tatgctttac
     5401 tggaattaaa ataaatcatg cagttgtaaa ggttactttt gaaagcaatt tagttaagga
     5461 gaaagaaaaa ccctcaaaat aaattccagc ttttctataa cttgatattc agtacataaa
     5521 agcagttgat atttatgatt gactgctttt tcttatcaaa aaggataggt tgtgcatttt
     5581 tacttgaaat agtttatgca catcaaaagt tggtgagcat gatattggaa atggtaagca
     5641 tgaaatcttg ataatacaag gaaataaatt taagttttca attaagtata tttccataat
     5701 ttagttaaca ataagctaat tattatttat attttgatat ataaataata attgtagcaa
     5761 attttaaaaa atcatatatt ttttgaacta aataaaaatt tatttttatt attttaaatt
     5821 agaggagatt c
//
LOCUS       DBGJFF010000187         5737 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.192, whole genome shotgun sequence.
ACCESSION   DBGJFF010000187 DBGJFF010000000
VERSION     DBGJFF010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5737
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.192"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            153..410
                     /locus_tag="ACOIAF_003591"
     CDS             153..410
                     /locus_tag="ACOIAF_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670571.1"
                     /translation="MIIIRSQDKTNLMQINQIKIDGSQVYAVFESKIDTVKIGDYENN
                     TRAIQVLDNIQNFIENGTKYDYITSNKVRYNVNKIFQMPAK"
     gene            complement(520..1200)
                     /locus_tag="ACOIAF_003592"
     CDS             complement(520..1200)
                     /locus_tag="ACOIAF_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HGZ2670572.1"
                     /translation="MIWTFYSFICVILPCLIYQIVVIKRKNLKVEKNVMIHLVWVYIF
                     LLYIYLALSKAGIGSIWDIGRYSGLFRIDEINLIPFDSVGILTYILNIIMFMPLGFLL
                     PLIWKNFRNIINVSLTGLGFSLAIELCQLFNLRATDIDDLMMNTLGAVLGYFIWVGVN
                     NLFNLIKKKDIFPSIYKNIIQDEIAITIDDDISKKITSLYKNEAIIYLILAVLGEFLL
                     FNWSIIPY"
     gene            1460..1759
                     /locus_tag="ACOIAF_003593"
     CDS             1460..1759
                     /locus_tag="ACOIAF_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670573.1"
                     /translation="MKWIKWYSITCICIFIVVAFYMFIFPNKIETIDTSSAYSFVEKK
                     VPNSAVYQGYKKNPVDGTTTIYYSYDNSTHIVRLSHPEDYSRKINWDKVSNIRFD"
     gene            2083..3738
                     /gene="ilvD"
                     /locus_tag="ACOIAF_003594"
     CDS             2083..3738
                     /gene="ilvD"
                     /locus_tag="ACOIAF_003594"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HGZ2670574.1"
                     /translation="MRSDIKKGIEGAPKRALMYGMGLTKEEIERPLIGIVNAQNEVIP
                     GHLHLDEIAEAAKNGVRMSGGLPLEFPAIGVCDGIAMGHVGMNYSLASRELIADSIEA
                     MAMAHGFDALVLIPNCDKIVPGMLMAAARLNIPSIVVSGGPMLPGKKNGKVYDFNSAM
                     EGVGACKDGTVSEEELEALAMNSCPGCGSCSGLFTANSMNCLTEALGMGIPYNGTAAS
                     HSGERKRMAKYAGMYVMELLKNDIKPRDILTIDAFKNAIAVDMAMAGSTNTVLHLPAI
                     AYESGIELNLDFFDEISEKTPCLTKLSPSGKHHIEDLHMAGGIPAIMNELSKINGINL
                     DCKTVTGKTIGENIGNCEIENEEVIHTLKNPYSNQGGLAILKGNLALNGAVVKKSAVA
                     EEMLVHEGPARVFNSEEEAVNAIFGKKINKGDVIVIRYEGPKGGPGMKEMLSPTSAVA
                     GMGLDKHVALLTDGRFSGATRGASIGHISPEAMEGGLIGLVEEGDIISINIPDKKLEL
                     KVDEVEIENRKLKFKPLEPKIKHGYLSRYAKLVTSANTGAVLK"
     gene            4082..4711
                     /locus_tag="ACOIAF_003595"
     CDS             4082..4711
                     /locus_tag="ACOIAF_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HGZ2670575.1"
                     /translation="MNREEKSKNSKEKIIQSAFSLFSSKGYDSTSTQDIINLSGLSRG
                     AMYHHFKTKEDILRSVTKELYSQMNNFLEYLVADDTLTANEKIIELVVHSANDYTRRK
                     MVHCSWLEKIPFALIEEVRNLNNVVAPNIAKIIKQGVENKEFSCEYPEELAEMLVFSI
                     DILLDPVLFKREYSEVCNRLDFLLFMLKKMDIPLIDEYGIQKFKDLFKQ"
     gene            4737..5549
                     /locus_tag="ACOIAF_003596"
     CDS             4737..5549
                     /locus_tag="ACOIAF_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGZ2670576.1"
                     /translation="MFYNAKSCQMMIGSNTVNYIEFGSGKKTLIILPGLGDGLFPLHG
                     KIQAIAFAFRYKQFAKDYKVYVFSRKNQITEKYSTRDMAKDQADIMKKLGIMKAEVMG
                     VSQGGMIAQYLAIDYPELVEKLVLAVTSSKQNDTIQNVICSWIDMAKKQNYNDLMIDT
                     AKKSYSERYLKKYQLFIPFLGKVGKPKDFKRFIIQATSCIEHNAFSELNKITCPTLII
                     GGANDKIVGNNASFHLAEKIKKSEIFIYEGLGHATYEEAQDFNERVLEFLNK"
BASE COUNT         2268 a          641 c          949 g         1879 t
ORIGIN      
        1 tttaagaaaa ttttttatta gagatgtaat tgatataaat aatcgtatta aaaaataaaa
       61 tactaagttt aaaaatttaa ataatctata aatcaattat aaaatattat ttattagaaa
      121 ttaaaatgaa ttaagtaaat ggaggaattg atatgataat aattagaagt caagataaaa
      181 ctaatttaat gcagataaac caaattaaaa tagatggtag tcaagtttat gcagtatttg
      241 aaagtaaaat tgatactgta aaaataggag attatgaaaa taatacaaga gctatacaag
      301 tgttggacaa tatacaaaac ttcattgaaa atggaacaaa atatgattat ataacatcaa
      361 ataaagtcag atacaatgtt aataaaatat ttcagatgcc agctaaatag tgagtgtgca
      421 ttttagttta ataactaaag cccactttct tttgtagtat gaaagagggc tttacatatt
      481 tgtaaatatc gaagtacact tattttatca ataaattatt taataaggta taatggacca
      541 attaaacaat aaaaattctc ctaaaacagc aagtattaaa tatataatag cttcattttt
      601 gtaaagagag gtaatttttt tgcttatgtc atcatcaatt gtaatagcaa tctcatcttg
      661 aataatattt ttatatatag atgggaaaat atcttttttc tttataagat taaataaatt
      721 atttacacct acccatataa aatatcctaa aacagcacct aatgtgttca tcattaaatc
      781 atcaatatct gtagctctta agttaaacaa ttgacaaagt tcaattgcta atgaaaaacc
      841 taatccagtt aatgaaacat tgataatatt tctaaaattt ttccaaatta aaggtagtaa
      901 aaatcctaaa ggcataaaca taattatatt taaaatatat gttaagatgc caactgagtc
      961 aaaaggtatt agatttattt catcaattct aaaaagccca ctataccttc ctatatccca
     1021 tatgctacca attccagctt tactaagtgc tagatagata tataataaaa aaatatatac
     1081 ccatactaaa tgtatcatta cattcttttc aactttcaaa ttttttcgtt ttataacgac
     1141 tatttggtat attaagcatg gtaatataac gcaaataaat gaataaaatg tccatatcat
     1201 tttattttgt cccctttcaa atttatgttt ttagttttat aagtactatc tttatattaa
     1261 actgtaagtt ttaaaaatct tttaataaaa tcttaaaaat ttattaattt ataaaaatat
     1321 attattaaga ttaaaaaata tatataatca aagtttctac aattatatag ttagggatat
     1381 aaaaatttta tcgtatgtat aaatattata aatatatgga aataataata gtatattttg
     1441 gtaaagtagg aggaatttta tgaaatggat taaatggtat agtattacat gcatatgtat
     1501 ttttatagta gtagcttttt atatgtttat atttcctaat aaaatagaaa ctattgatac
     1561 ttcatctgca tatagctttg ttgaaaagaa agtaccaaat agtgctgtgt atcaaggata
     1621 caaaaagaat ccagttgatg gtactactac aatttattac agctatgata attcaactca
     1681 tatagttaga ttatcacatc cagaagacta ttcaaggaag attaattggg ataaagtttc
     1741 aaacatcaga tttgattaat ctttaatatt tataaaagtc tatatatcta aggcaaatat
     1801 attattaatc ttattcatag ttgagcactt tctattatag aagtgcttct tctgatttat
     1861 atcataaaat attactcaac aatttgtaaa atatattagt ttataaaata tattgaaaag
     1921 ataatattat ataatacgat tgtgtaagat attgataaaa aatcgtaata aattttcatt
     1981 agtatatgct aatatttttt tacaattttc gtaattaatt aatagaaaaa tatattataa
     2041 taatattatg aaaatttaaa aaagtaagaa agaggtaata ttatgagaag tgatataaaa
     2101 aaaggaatag aaggagctcc aaagagagcg ttaatgtatg gaatgggtct tactaaagaa
     2161 gaaattgaaa gaccattaat aggtatagta aatgcacaaa atgaggtgat tcctggtcat
     2221 ctgcatcttg atgaaatagc agaagctgca aaaaatggag taagaatgag tggtggtttg
     2281 ccattagaat ttcctgctat aggggtatgt gatggtatag ctatggggca tgttggaatg
     2341 aattattccc tggcttcaag agaattaata gcagattcaa tagaagctat ggctatggca
     2401 catggttttg atgctttggt acttatacct aattgtgaca agatagtgcc aggtatgtta
     2461 atggctgctg caagacttaa tataccaagt attgttgtga gtggagggcc tatgcttcca
     2521 ggtaaaaaaa atggaaaagt atatgatttt aactctgcaa tggagggtgt tggagcatgt
     2581 aaagatggaa cagtaagtga agaagaatta gaagcgttag ccatgaactc ttgtcctgga
     2641 tgtggttcat gttctggtct tttcacagca aatagcatga attgtttaac agaagcttta
     2701 ggaatgggta taccatataa tggtactgct gcgtcacatt ctggagaaag aaaaaggatg
     2761 gcaaaatatg caggtatgta tgttatggag ttacttaaga acgacataaa acctagagat
     2821 attttaacaa tagatgcttt taaaaatgct atagctgtgg atatggcaat ggctggttct
     2881 acaaatacag tacttcactt acctgcaata gcttatgaat caggaataga gcttaactta
     2941 gatttttttg atgaaataag tgaaaaaact ccttgtttaa caaaattaag tccaagtgga
     3001 aaacatcata ttgaagattt acatatggca ggaggaatac cagctataat gaacgaactt
     3061 tcaaagataa atggaataaa tttagattgc aaaaccgtaa caggcaagac tataggggaa
     3121 aatataggaa attgtgaaat agaaaatgaa gaagtaatac atacattaaa gaatccatat
     3181 agtaaccaag gtgggcttgc aatattgaaa ggaaatcttg ctctaaatgg agctgttgta
     3241 aaaaagtcag cagttgcaga agaaatgtta gttcatgaag gaccagcaag agtttttaat
     3301 tcagaagaag aagctgtaaa tgctattttt ggtaaaaaaa taaacaaagg tgatgttata
     3361 gttataagat atgaaggtcc aaagggtggt ccaggaatga aagaaatgct atctcctaca
     3421 tcagcagttg caggaatggg acttgataag catgtagcac ttcttactga tggtcgtttt
     3481 tcaggggcaa ctagaggagc atctataggc catatttctc cagaagctat ggaaggtggt
     3541 ttaatcgggt tagttgaaga aggagatata atttctataa acataccaga caaaaaatta
     3601 gagttaaaag tagatgaagt tgaaatagaa aatagaaaat taaaatttaa acctttagaa
     3661 ccgaaaataa agcatggata cttaagtaga tatgctaaat tggtaacatc agcaaataca
     3721 ggagcagttt taaaatagta gactatattt atgtaaatta gaaaaagtta agaaaatatt
     3781 aattggatat cttaggtgtt atagcactaa tgaggtatcc tttttatttt gtatgaaaaa
     3841 cacagcaaaa tcttgattat ctttgaaaac aatacatctc tattataagg aatacgagta
     3901 cagcatagag atgtatcttt tagaccttgt tgaggtaata caacttttta aaagtttgat
     3961 gtggaatgct tttcgttcca tctgaactat gaagaaattg aaagtcagtt aattcttgac
     4021 ataataaata ttaaaatgta taataaaaaa caaacagaca gtctgttttt aggagggaaa
     4081 tatgaataga gaagaaaaaa gtaaaaatag taaagaaaaa attatccaat cagcattttc
     4141 actattttcg tctaaaggat atgattcaac atctacacaa gatattatca atttatctgg
     4201 tctatctaga ggtgcaatgt atcatcactt taaaactaaa gaagatatac tgagaagtgt
     4261 cacaaaagaa ctttactcac aaatgaataa ttttttagag tatcttgttg ctgatgacac
     4321 ccttacagca aatgaaaaaa taatagaatt ggttgttcat agtgcgaatg attacacacg
     4381 tagaaaaatg gtacattgta gctggttaga aaaaatccca ttcgctttaa tagaggaagt
     4441 tcgtaatctc aacaatgtag ttgcacccaa tattgctaag ataattaaac aaggtgttga
     4501 aaataaagaa ttttcttgtg aatatccaga ggaattagct gaaatgctcg tttttagtat
     4561 tgatatttta cttgaccctg tattatttaa acgtgaatac agtgaagtat gtaataggtt
     4621 ggatttttta ttattcatgt taaaaaaaat ggatattcct ctaattgatg agtacggtat
     4681 tcaaaaattt aaagatttat ttaaacaatg ataataggaa aagtgaggaa tagactatgt
     4741 tttataatgc aaaaagctgt caaatgatga taggaagtaa tacagtgaat tatattgaat
     4801 ttggtagtgg taagaagaca ctaattattt taccaggatt aggagatggt ttatttcctt
     4861 tacatggaaa aatacaggcg attgcttttg cttttagata taagcaattt gcaaaagatt
     4921 ataaggttta tgtatttagt agaaaaaatc aaattacaga aaaatattcc acaagggata
     4981 tggctaaaga ccaagcagat ataatgaaaa aacttggaat tatgaaagca gaagtaatgg
     5041 gtgtttctca aggtggaatg atagctcaat atctggcaat tgattatcct gagttagttg
     5101 agaaacttgt gttggcagtt acttcttcaa agcaaaatga tactatccaa aatgttattt
     5161 gtagctggat tgatatggcg aagaagcaga attacaatga tttaatgatt gacacagcaa
     5221 agaaatccta ttctgaaaga tatttaaaaa aataccaatt atttattcct tttttaggaa
     5281 aagtaggtaa accaaaggat tttaaacgat ttattattca agcgacctct tgcatagaac
     5341 ataatgcttt ttccgaatta aataaaataa catgtccaac cttaattata ggaggtgcta
     5401 atgataaaat tgttggaaat aatgcttctt ttcatttagc agaaaaaatt aaaaaaagcg
     5461 aaatatttat ctatgaggga ttgggacacg ctacatatga agaagcacag gattttaatg
     5521 aaagagtttt agagttttta aataagtaaa cacctttgtt aaaaatatac atatgaagat
     5581 tggaaattta atgttaaaaa tagaaacatg aaaatatgct taactggtat ttttactatt
     5641 cataaccaat ttttgataca ttatctacta taaatacaaa tatagcttca atgtgattat
     5701 atattgttgt attggtaaag cacttataca aacagag
//
LOCUS       DBGJFF010000188         5580 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.193, whole genome shotgun sequence.
ACCESSION   DBGJFF010000188 DBGJFF010000000
VERSION     DBGJFF010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5580
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.193"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(207..1304)
                     /locus_tag="ACOIAF_003597"
     CDS             complement(207..1304)
                     /locus_tag="ACOIAF_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HGZ2670577.1"
                     /translation="MKKTYLFSPGPVMVTDKVRNSLLHYDICHRGNEFMNLFKDTQQK
                     INKLYNATSNYYSLIVSGSGTSVNECVLSSIFDKEDAVLLVSNGVFGERLEEILSTYN
                     VKTYKPNFEWAEYPDLDILEKYIIENSDIKVIAMVFHETSSGMINPVPELGKLAKKYN
                     KIFFVDAISASAGEYIDVEEFNIDIITGVGGKALGAFPGSAYLCAKESILQNIKENQC
                     KNVYLNLYKHYKVAKSSSQTPNTPNVTLIFALNEALTEFLEDDSKIERYKECSAILRN
                     GMEELGLTFLLPDKYMSNTVTSVFLPKEINVNEFILGLERESGYVVYPGKGKFLDANI
                     FQVANMGEIYPEDCYKFLDILKSKLEIKKVI"
     gene            1597..3123
                     /gene="glpK"
                     /locus_tag="ACOIAF_003598"
     CDS             1597..3123
                     /gene="glpK"
                     /locus_tag="ACOIAF_003598"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HGZ2670578.1"
                     /translation="MKYVLAIDQGTTGTRAVLINEKSEFVFSDYMEHTQIYPQSGYVE
                     HDPIEIWNNTKTVSNNVLQQAFENGINESDIKGIGIDNQGETVMLWDKNTGKPLYNAI
                     VWQCRRTYDYVENLKNIPGLENKIIDKTGLIIDPYFSGTKIKWVIDNVKGVKEKIKNG
                     EVLAGTLDSWLIWKMTGGKSFLTDVSTAARTMLFNIRDMDWDRELLDIIGVPRSILAD
                     IMPTSGDFGTTDKEAFCGVSIKITASMVDQPAALFGNGCFEPGMVKNTYGTGCFLYMN
                     IGEELNIAGNGILTTVAWKIGDKVTYAYDGGVYIAGAAIQWLRDGIGVIKNYSETDDM
                     ANSISSTGGVYFVPAFAGIAAPYWDQYARGTMVGITGSTKKEHIVRATLESVALQVKD
                     VVDSMNLASGKKIKMLRVDGGITKNNFTMQFQSDILGIPIEIAENPETTVLGVGYMAG
                     LSCGVWNSIDEIRSEFTISKKYIPNMTTQKREEILLGWNQAVKRAMNWENDTKKVCAN
                     "
     gene            3164..4279
                     /locus_tag="ACOIAF_003599"
     CDS             3164..4279
                     /locus_tag="ACOIAF_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HGZ2670579.1"
                     /translation="MPFDKIILYIMAVGVLLGALDRIIGNKFGLGEQFEEGFNSMGPL
                     ALGMVGIVCLAPVISDVLGPIIIPIFNLCGADPSMFATILANDMGGYPLAMELAHNKE
                     AGMLAGLVVSSMLGCTLVFSIPVGLGLIEYEDRPFFAKGLLIGLITIPFGGIIGGLIA
                     GFNLSMVIINMIPVIILAVILAVGLKFNSTLMINGSLVFGKFISILITIGLGAAAFQE
                     ITGIVLIKGMTPIREGIQVIGSIAIVLLGTFPILHLLVKVLNKPLTIVGGKLGMDATS
                     AAGLVFTLANSIPVYKMMKDMSPKGKVVNTAWLVCATAALGDHLGFTAGVVPEMITPV
                     VIGKLVGGVLAIALAILMTKDLTLEEEQSIKMKKDAV"
     gene            complement(4560..5324)
                     /locus_tag="ACOIAF_003600"
     CDS             complement(4560..5324)
                     /locus_tag="ACOIAF_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HGZ2670580.1"
                     /translation="MLPQERYEKILSILKSDNIIKINDIVQMFDISIETARRDLAHME
                     SIGLLKRVYGGAIPVIASSVELNYVKRKKINSNDKKQIAIKCSEFINTNDTIFMDTGT
                     TVLEVSKNLKDKKNLTVITNSILVANELMNTNIKVYVLGGYLRETEGSFSGPLTLSAI
                     EQFNVDKAILGAGGITLENGISDYHLEEALVRKKMIERSRKSIVVVEPPKFGINSFAS
                     VAPIEHIDTIITSASIDHNIIIEFSQKGIPLVIADN"
BASE COUNT         2053 a          708 c          940 g         1879 t
ORIGIN      
        1 aataaaaaac gattgcgtca aaaatataaa ataaattaaa agaaaaagat tatattgaag
       61 gaaaaagatt tgaaataggg gtgaacttta catattatag ttcaccccta tacaaaaata
      121 taaaatataa tttattatag gaaaatttaa aaaaatcagt ctatttaatt tatactgata
      181 tattgtagga ttttacaaac aaactgttat ataacttttt ttatctccaa ttttgatttt
      241 aagatatcta agaatttata acaatcttct ggatatattt ctcccatatt tgctacttgg
      301 aatatattag catctaggaa tttgcctttt cctggataga caacatagcc actttccctt
      361 tctagcccta atataaactc atttacattg atttctttag gtagaaatac agatgttact
      421 gtattagaca tatacttatc tggaagtaaa aatgttagac ccaattcttc cataccattc
      481 cttaaaatag cagaacattc tttgtatctt tctattttac tgtcatcttc caaaaactca
      541 gttaaagctt cattaagggc aaatattaaa gtaacattag gtgtatttgg tgtttgagaa
      601 gatgatttag caactttata gtgtttataa agattaagat acacattctt acattgattt
      661 tctttaatat tttgtaaaat actctcttta gcacaaaggt atgcagaacc tggaaatgct
      721 ccaagagctt taccaccaac acctgttatt atatctatat taaactcttc cacatcaata
      781 tattcacctg ctgaggcact tattgcatct acaaaaaata ttttgttata cttcttagca
      841 agttttccta gttcagggac gggatttatc attcctgaac tagtttcatg gaacaccata
      901 gcaattactt ttatatcaga attttcaatt atgtattttt ctaaaatgtc taggtctggg
      961 tattctgccc attcaaaatt aggtttatat gtttttacat tataagtaga taatatttct
     1021 tctagccttt ctccaaaaac accattacta actagcagaa ctgcatcttc tttgtcaaag
     1081 atagaagata gtacacattc atttacagat gttccagagc cagatacaat taatgagtag
     1141 taattagaag ttgcattata tagtttattg attttttgtt gtgtatcttt gaataaattc
     1201 ataaactcat ttcctctatg gcatatatca tagtgaagaa gtgaatttct gactttatct
     1261 gtaaccatta ctggtccagg actgaataga tatgtttttt tcattgtaaa gcctccttaa
     1321 aaagttaaat ttaaattttt gaatgactga ttatgtgtaa atattaaaat attatgtgtt
     1381 attttaacta gattatagaa taaaataata aatattgcaa taatatttca ataaaaatac
     1441 ttttttaatc actataatta aaaaaaacaa aaaatattgt aatgaatatt gcataaatcc
     1501 acatgatgat ataaaatata aataacttga atagattata tattcaaaaa ataaaaaaga
     1561 atttattaat tctgaaaata aagggggaca tttaaagtga aatatgtatt agcaattgac
     1621 caaggaacaa caggaacaag agcagtattg ataaacgaaa aatcagagtt tgtttttagc
     1681 gattatatgg aacatactca aatatatcca caatctggat atgttgaaca tgaccctata
     1741 gaaatttgga ataatacaaa aactgtgagt aataatgtat tacaacaggc ttttgaaaat
     1801 ggaatcaatg aaagtgatat aaaaggaatt ggtatagata atcaaggtga aacagtaatg
     1861 ttatgggata aaaatacagg taaaccactt tacaatgcta ttgtgtggca gtgcaggaga
     1921 acttatgatt atgtggaaaa tttaaagaat ataccaggtt tagaaaataa gataatagat
     1981 aagactgggt taattataga cccatatttc tcaggtacaa aaattaaatg ggtaatagat
     2041 aatgtaaaag gtgtaaaaga aaaaattaaa aatggagaag tacttgcagg aacattagat
     2101 agttggttaa tatggaaaat gacaggagga aaatcttttt taactgatgt atctacagca
     2161 gcaagaacaa tgctttttaa tataagagat atggattggg atagagagtt gcttgacatt
     2221 ataggagtac ctagaagtat tttagcagat ataatgccta cgagtggtga ttttggaact
     2281 acagataagg aggcattttg tggggtttct ataaaaatta ctgcttcaat ggtagaccaa
     2341 ccagcagctt tatttggcaa tggatgtttt gaaccaggaa tggttaaaaa tacttatggt
     2401 acaggatgtt tcctatatat gaatataggt gaggaattaa atatagcagg taatggtatt
     2461 ttgacaactg ttgcttggaa aataggagat aaagttacat atgcttatga tggtggagtg
     2521 tatatagcag gtgctgcaat acaatggctt agagatggca ttggagttat aaaaaattat
     2581 tcagaaactg atgacatggc aaattccata tcaagtactg gaggtgtata ttttgtacca
     2641 gcatttgcag gtatagcagc accttattgg gaccaatatg ctagaggtac aatggttggg
     2701 ataacaggaa gtactaaaaa agagcatata gtaagagcga ctcttgagtc tgttgcactt
     2761 caagtcaaag atgttgttga ttctatgaac ttagcctcag ggaaaaaaat aaaaatgttg
     2821 agagtagatg gtggaattac taaaaataac tttacaatgc aatttcaatc cgacatatta
     2881 ggtataccaa ttgaaattgc agaaaatcct gagacgacag tacttggagt aggatatatg
     2941 gcaggtcttt cttgtggtgt ctggaacagt attgatgaaa ttagaagtga atttacaata
     3001 agcaaaaaat atattccaaa tatgactact caaaaaagag aagaaatatt acttggatgg
     3061 aaccaagcag taaaaagagc tatgaattgg gaaaacgata ccaaaaaggt gtgtgctaat
     3121 taagttacaa tagagtattt tacatgtata ggaggaatta aaaatgccct ttgataaaat
     3181 tatattatac ataatggctg tgggagtgct tttaggagcg ttagatagaa taattggaaa
     3241 taaatttgga cttggagagc agtttgaaga agggtttaat tcaatgggtc cattggcttt
     3301 aggaatggtt ggtatagttt gtttagctcc agtaatttca gatgtactag gtcctataat
     3361 aataccaatc ttcaatctct gtggagctga cccatctatg tttgcaacta ttttagcgaa
     3421 tgatatggga gggtatccat tggctatgga acttgcacac aataaagagg caggaatgct
     3481 tgcaggattg gttgtgtcat ccatgcttgg atgtacatta gtattttcaa ttccagtagg
     3541 actaggtctt atagaatatg aagacagacc attctttgca aaagggttac tgataggctt
     3601 gataacaata ccttttggag gtattatagg aggattaata gcaggattta acttatcaat
     3661 ggtaattata aatatgatac cagtgataat attggcagta atattggcag taggtttaaa
     3721 atttaattct acgcttatga taaatgggtc attagttttt ggaaaattta tatctatatt
     3781 gataacaata ggtttgggag cagcagcatt tcaagaaata acaggtatag tacttataaa
     3841 aggcatgaca cctataagag aaggtattca agtaattgga agtattgcaa tcgtgttatt
     3901 aggtactttt ccaatattac atttattagt taaagttctt aataaaccac ttacaatagt
     3961 gggtggaaaa ttaggtatgg atgccactag tgcagcagga ttagttttta ctttagccaa
     4021 tagtattcca gtatataaaa tgatgaaaga tatgagtcca aaggggaaag tagtaaatac
     4081 tgcttggctg gtttgtgcta cagcagcttt gggggaccat ttaggattta cagcaggagt
     4141 agttccagag atgattacac ctgttgtaat tggaaaattg gttggaggag tattggcaat
     4201 tgcacttgct atacttatga caaaagattt aaccttggaa gaagaacaaa gtattaaaat
     4261 gaagaaagat gcagtgtaga ataaacaata cttaagtcaa atttaaatta ttttaaagta
     4321 ggagtttaaa taactaaaaa taaattaaaa agacagacaa ttaaaaaata atcgaataac
     4381 taaaacttat aaattaaata aaatggcaat caaataacta aaaatgataa gctaactgaa
     4441 agtagttaca taattaaaat ataaagtata gtttagacat aaatggagct aaataaataa
     4501 ttatttggct ccttaatttt aatttattac atcaaggaac taaaaatcaa tcatataagc
     4561 taattatcag ctatgactaa aggtattcct ttttgagaaa actctattat tatattatgg
     4621 tctatactag cagaggtaat aatagtatct atatgttcta taggagctac tgatgcaaat
     4681 gaatttatac caaacttagg aggttcgaca actactatag attttctaga cctttcaatc
     4741 attttttttc taacaagtgc ttcttctaag tgatagtcag agattccatt ttcaagagtg
     4801 atacctccag ctcctaaaat agctttatca acattaaatt gttcaatagc tgacagtgta
     4861 agcggaccag aaaaagaacc ttcagtttct ctaagatagc ccccaagcac atatactttt
     4921 atattagtat tcataagttc gtttgcaact aaaattgagt tggttattac agttagattc
     4981 tttttatctt tcaaattttt tgaaacttca agtacagtag ttccagtatc cataaaaata
     5041 gtatcatttg tatttataaa ctcactacat ttaatagcaa tctgtttttt atcgtttgag
     5101 tttatttttt ttcttttaac atagttaagt tctacgctac ttgctataac tggtatagca
     5161 ccaccataaa ctctttttaa aagaccgata ctctccatat gggctaaatc tcttcgtgca
     5221 gtttctattg atatatcaaa catttgaact atgtcattta ttttaattat attatctgat
     5281 ttgagaatac ttaaaatctt ctcatatctt tcctgtggta acataaaata tcctccttgg
     5341 actagtatta ttttagtttt ggcataactt acaattaagt cagagtttag gtatatttgt
     5401 taatgtatgt atatttatca aataatatta taagtgttat gcaagataat tttaccatat
     5461 ttaaaactta catttaagca aaaaagtaaa aatagatgtt taataattca aaataactca
     5521 aattttataa aacaattgac taaaatatga ttttattata taatttacta gtatagagat
//
LOCUS       DBGJFF010000189         5578 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.194, whole genome shotgun sequence.
ACCESSION   DBGJFF010000189 DBGJFF010000000
VERSION     DBGJFF010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5578
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.194"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            102..1283
                     /locus_tag="ACOIAF_003601"
     CDS             102..1283
                     /locus_tag="ACOIAF_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HGZ2670581.1"
                     /translation="MNKKYIKAGNLIDGTGNSIMQNKGIIIDGDTIVEIEDIRDDLNG
                     YDVYDYLDKTVMPGIMNCHVHLTMEPVGNPKIYYDNVSDVELVVNTIKQLDAYLDSGV
                     TYIRSLGCPKYVDVQLKNLIDKGSIDGPGIVTSGPVICMTGGHGYYFGIESDGVDECR
                     KSARTVLKNGVDCVKIMATGGVTTDGVEPGSPQLTLEEMKAAVDEAIKAGKITATHAQ
                     GRTGIKNAVLAGITSIEHGVYLDDEIIDLMLERGTYLVPTVAAPYFILNAGKESGITE
                     ATLRKCEIVAIPHKESFLKAYKKGIKIAVGSDAGTSYNEHGKTYYEMKLMSDYGMDNM
                     DIIVAATKTASELLRIDKNYGTLEKGKKADVIVVNGNPVENIDVLADVLAVFKLGKKV
                     R"
     gene            complement(1425..2342)
                     /locus_tag="ACOIAF_003602"
     CDS             complement(1425..2342)
                     /locus_tag="ACOIAF_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotA family spore coat protein"
                     /protein_id="HGZ2670582.1"
                     /translation="MENNKCREDFRFTQEYEEDYPNTNERYYENYQVADRYYNYPNKY
                     KEPKIKQCCCKKSMREALELLRYDALRPFVNFNQFAFISDFFIVGANLVGIDLSAPPK
                     DNLSGLDGTFERFSACNCDLIDIAGRVSYPIPVPLTLEGLINTIGTIPGVAELIALID
                     AVIPPTIDLGAILDAILAAIIDFILAASTPLANVDLASLCNLKAVAFDITPADYEDFI
                     ASLGYYLDKKHYKECNCNCDCDDCCCNKGILDNLYMSNINNQVTVVAGSLVLTGVEVL
                     GKKNDVIVLGNSNDSRIYFVCVDSIDYIA"
     gene            2595..3389
                     /locus_tag="ACOIAF_003603"
     CDS             2595..3389
                     /locus_tag="ACOIAF_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2670583.1"
                     /translation="MIEVDKLCKSFTRVVKDDKNKSSIRKLKKVKTKKEEFFAVNNVS
                     FKVMEGEIVGILGPNGAGKTTLLRMLGGILTPTSGSINISGYDYSIDRNSAKKEIGYL
                     SGNTKLYGRLSPRELLTTFASLYEMSKEDIEESIENIVKIMDMSEFIDNRIENLSTGQ
                     TQRTSIARCLIHSPKVYIFDEPTLGLDVLSSASIIDFMKNEKLRGKTVLYSTHYMEEA
                     ETLCDKIFMIHNGKIIASGTPQSLKEESGVNNLRDVFIKLARKEDI"
     gene            3390..5450
                     /locus_tag="ACOIAF_003604"
     CDS             3390..5450
                     /locus_tag="ACOIAF_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit/CPBP
                     intramembrane protease"
                     /protein_id="HGZ2670584.1"
                     /translation="MRSKIVKYIFKKEMLDILRDKKTLFMMIVVPLLLYPLLMLLMIQ
                     VMNMSASSMNSKDINIAFNNKPNKVLVSMIEDDNLEKDTKNTSNDKGKIRVLDVDNYK
                     KSLEENKIDAYIKMEEKKSSINYQIYINSSQENSSNALKRIETVLDKYKRFTIEKTLS
                     EKGLDVQATLEPITYSTVDVAKTEEVAGYFLGQILPFILIIGVLLGSIYPAIDVMAGE
                     KERGTLETLFTLPISNLELVMGKYMAVSLSAIVTAILNILSMGLTMAFILVSGGISSQ
                     FGFGNFNYGDLVLPIITTLICICLFSMVVSAISMCVCSLAKSFKDAQNYITPLMLIIM
                     IPSYVSMIPNIVLDRVTAVIPVVNISLLIKSVLSFKSDLNLIALVLASNVAFVILAII
                     LLSKMFNSEEILFGNSKSFSFLEKRSNIKKGSIPTVSDGVILYAVGLILLIYVGSLVQ
                     IKFGMAGIIMTQFMIISLPLLFAYYIKADFKEVFSLTVPKLKHVFGSIVLWIGGYILI
                     MIITQLILFLFPQNMEVAESLNKALFIKDSVLLNLLAIALLPAICEEMFFRGFIFSSF
                     SKSKDKNKSIKLAIICSGVLFGIMHMDFIRIIPTSILGIIFAYSVYKSGSIFVSILLH
                     FLNNSVAVVLNHYTTGNITNLYKFVEVDFSNLNVLKFALILLISLILIMLGLRVLDRK
                     SLRN"
BASE COUNT         2136 a          630 c          912 g         1900 t
ORIGIN      
        1 taaatttgaa atagaatgct tatagtggta aaattaatta ttagaatatt agaaataata
       61 aataaataat acattgtact taaaaggggg agaaatttaa gatgaacaaa aaatatatta
      121 aagcagggaa tttaattgat ggaaccggaa atagtattat gcaaaataag ggtataatta
      181 ttgatggaga tactatagtt gaaattgaag atataagaga tgatttaaat ggatatgatg
      241 tgtacgatta cttagataaa actgttatgc caggcattat gaattgtcat gttcacttga
      301 caatggagcc agttggaaat ccaaagatat attatgataa tgtatcagat gtagagcttg
      361 tagtaaatac gataaaacaa cttgatgctt atttggattc aggagtaaca tatattagaa
      421 gcttaggttg tcccaaatat gtggatgtac aacttaaaaa tcttatagac aaagggtcta
      481 tagatggtcc tggtatagtt acttcaggtc cagttatatg tatgacagga gggcatggat
      541 attattttgg tattgaaagt gatggagtgg atgaatgtag aaaatcagcc agaacagttt
      601 taaaaaatgg agtagattgt gtaaagataa tggcaactgg aggtgtaaca actgatggag
      661 ttgagccagg gtcaccacaa ttaactttgg aggaaatgaa ggctgctgta gatgaagcta
      721 taaaagcagg taaaataact gccacacatg cacaaggaag aactggtata aaaaatgcag
      781 tgcttgcagg aattacttct atagaacatg gtgtttattt agatgatgaa ataattgatt
      841 tgatgcttga aagaggaact tacttagttc caactgtggc tgcaccatat tttattctaa
      901 atgcaggaaa agaaagtgga ataactgaag ctactcttag aaagtgtgag attgttgcca
      961 ttccacacaa agagtcattc ctaaaagcat ataaaaaagg aattaaaatt gcagttggtt
     1021 ctgatgcagg aacttcttat aatgaacatg gaaaaacata ttatgaaatg aaactaatgt
     1081 cagattatgg tatggataat atggatataa tagttgctgc tacaaaaaca gcatctgaac
     1141 ttttaagaat tgataaaaat tatggaactc tagaaaaagg aaagaaagca gatgttatag
     1201 ttgtaaatgg aaatccagta gagaatatag acgtacttgc agatgtgtta gcagtattta
     1261 aacttggtaa aaaagttaga taaatagtat aaaaataaaa aagagtgtct aaagaaattt
     1321 ttaaataatt atagacactc ttttttatta gtaataatta aatcttttat actttagaaa
     1381 atagattcta ttttatattt agatataact ctttaaaatc attcttatgc aatataatct
     1441 atagaatcta cacatacaaa gtatattctt gaatcattag aatttccaag tactataaca
     1501 tcatttttct tacctagaac ttcaacacca gttagaacca aactaccagc tactacagta
     1561 acttgattat ttatatttga catataaaga ttatctagga tacctttatt acaacagcaa
     1621 tcatcacaat cgcagttaca attacattct ttgtaatgtt ttttatcaag atagtaacct
     1681 aaagatgcta tgaaatcttc ataatctgca ggtgtaatat caaatgcaac agctttaaga
     1741 ttacacaatg atgctaaatc tacgtttgct aatggagtag atgcagcaag tataaaatca
     1801 attatagcag caagtattgc atctaatata gccccaaggt ctatcgtagg aggaataact
     1861 gcatcaataa gtgcaattaa ttcagctact cctggtatag ttcctatagt attaattaat
     1921 ccctcaagag ttaaagggac tggaatagga taagatactc taccagctat atctattaaa
     1981 tcacagttac aagcagaaaa tctttcaaaa gtaccatcaa gtccagataa attatctttt
     2041 ggaggagctg aaagatctat acctaccaaa ttagcaccta ctataaagaa atctgagata
     2101 aaagcaaatt gattaaagtt tacaaaaggt cttagagcat catatcttag aagttctaag
     2161 gcctctctca tacttttttt acaacaacat tgttttattt taggttcttt atatttattt
     2221 ggataattat agtatctatc agctacttga taattttcat agtatctttc atttgtattt
     2281 ggataatctt cctcatattc ttgtgtaaat ctaaagtcct ctctacattt attattttcc
     2341 actaaataca ctccttttat attttaattt aaatgtatag ataattaagc ttatctatat
     2401 agtaatatta tatttataat gtaaaaaaat gttacaaaag atattataaa caaataaaaa
     2461 ataataattt gtcagataaa cttgtaaatt tttatgaata atccattcta aaacaaaaaa
     2521 ctgtgtataa aaaaggttta tatgttatat ttatataaat ataacatatg aacgagaaag
     2581 gagtggtttg ataaatgatt gaggtagata aattgtgtaa gtcttttaca agggttgtaa
     2641 aagatgataa gaataaaagt tcaataagaa agctaaagaa agtaaaaact aaaaaagaag
     2701 aattttttgc tgtaaataat gtttcattta aagttatgga aggtgaaatt gttggaatat
     2761 taggtccaaa tggtgcagga aagactacat tacttagaat gttaggtggc atattaacac
     2821 caacatcagg aagtataaat atttctggat atgattattc aatagataga aattcagcaa
     2881 aaaaagaaat aggttatctc tctggaaata caaaactata tggaaggctt tctccgagag
     2941 aacttctaac aacttttgca agtctatatg agatgtcaaa agaagatata gaagaatcaa
     3001 ttgaaaatat tgtaaaaata atggatatga gtgaatttat agataataga attgaaaatc
     3061 tatcaactgg tcagactcaa agaacatcta tagctcgttg tttaatacat tctccaaaag
     3121 tatatatttt tgatgagcca acactaggtc tggatgtttt aagtagtgca tctattatag
     3181 attttatgaa gaatgaaaaa ctaaggggaa aaactgttct ttactcaact cattatatgg
     3241 aagaagcaga aactttatgt gacaagattt ttatgattca caatgggaaa ataattgcat
     3301 caggaacacc acagagttta aaagaggaat ctggagtaaa taatttgaga gatgtattca
     3361 taaaacttgc cagaaaggag gacatttaaa tgagaagtaa gattgtaaaa tacatattta
     3421 aaaaagaaat gttagacata ttgagagata aaaagacttt atttatgatg attgttgtgc
     3481 cacttctttt atatccttta cttatgttat taatgattca agtaatgaat atgtctgcaa
     3541 gtagtatgaa tagtaaggac attaatatag cttttaataa taaacctaat aaggttttag
     3601 tatccatgat tgaagatgat aatttagaaa aagatactaa aaatacttca aatgataaag
     3661 gcaaaataag agtgttagat gtagataact ataaaaagtc attagaagaa aataaaattg
     3721 atgcttatat aaagatggaa gaaaaaaaat catctataaa ttatcaaata tatataaatt
     3781 cttctcaaga aaattcttct aatgcactaa aaagaattga aactgttttg gataaatata
     3841 aaagatttac catagaaaaa actttatctg aaaaaggatt agatgtacaa gctactttag
     3901 aaccaataac atatagcact gtagatgttg caaagacgga agaagtggca ggatactttt
     3961 taggtcagat acttccattt atattaataa taggagtttt actaggttca atatatccag
     4021 caatagatgt tatggctgga gaaaaggaaa gaggtacatt agaaacatta tttacactcc
     4081 caatatcaaa cttagaactg gttatgggaa aatatatggc agtttctctt agtgcaatag
     4141 taacagcaat tttaaacatt ttatctatgg gactgactat ggcatttatt ttagttagtg
     4201 gaggtattag tagccaattt ggttttggaa actttaatta tggagattta gtcttaccaa
     4261 ttataactac tttaatttgc atatgtcttt tttcaatggt agtttcagca ataagtatgt
     4321 gtgtatgttc attagcaaaa agttttaaag atgctcaaaa ctacataaca cctcttatgt
     4381 taattattat gataccatca tatgtttcta tgataccaaa tatagtatta gatagagtaa
     4441 cagctgtaat acctgtagta aatatatctt tacttataaa aagtgtatta tcttttaaaa
     4501 gcgatttaaa tttaatagcc ttagttttag catcaaatgt agcctttgtt atattggcaa
     4561 tcatactact gtctaagatg ttcaattctg aggaaatact ttttggtaat agtaagagtt
     4621 tctctttctt agagaaaaga agtaatataa aaaaagggtc tataccaact gtaagtgatg
     4681 gtgttatttt atatgcagta ggattaattc tattgattta tgttggttct ttagttcaaa
     4741 ttaaatttgg tatggcaggt attataatga cacaattcat gataatttct ttaccattat
     4801 tatttgcata ctatattaaa gctgatttta aagaggtatt tagcttgaca gttcctaaat
     4861 taaagcatgt gtttgggagc attgttcttt ggattggtgg atacatactt attatgatta
     4921 taacacagct aattttattc ttatttcctc aaaatatgga agttgctgaa agtttaaata
     4981 aggctctttt tatcaaagat agtgttttac tgaatttact agcaatagct ttattaccag
     5041 ctatatgtga agaaatgttt tttaggggat ttatattttc ttcatttagt aaatcaaaag
     5101 ataaaaataa gtctatcaag ctagctataa tatgcagtgg ggtactattt ggtattatgc
     5161 atatggattt tattagaata attccaacat caatattagg tattatattt gcatatagtg
     5221 tgtataaatc tggttcaata tttgtatcaa tattattgca ttttttaaat aatagtgtag
     5281 cagttgtgtt aaatcattat acaactggaa atataaccaa cttatataaa tttgttgaag
     5341 ttgatttttc gaacttaaat gtacttaaat ttgccttaat tcttctaata agtcttatat
     5401 taattatgtt aggattaaga gttttagata gaaagtcttt aagaaattaa gtataaagtt
     5461 attatataga ttaaaatatt aataagcact aaagtatatg gatatatctt gtatataaaa
     5521 atactttagt gctattttat atattttaat tatatgaatt atttatcgtt tcatttat
//
LOCUS       DBGJFF010000190         5553 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.195, whole genome shotgun sequence.
ACCESSION   DBGJFF010000190 DBGJFF010000000
VERSION     DBGJFF010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5553
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.195"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            240..1067
                     /locus_tag="ACOIAF_003605"
     CDS             240..1067
                     /locus_tag="ACOIAF_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HGZ2670585.1"
                     /translation="MDLSKKVKILENASDGKSEFEILEFEKLEGASSPLMAMGLYFAR
                     EAGLKARQIKIKLNNSSIKTEAGALYYYKGNISSETKIGGVGGLFKKGISGALTNESM
                     MKPVYKGTGEVYLEPSFRHYLFMSLDNDSIIVDKGLFYCCSDTIEVKAFTQKNFSSAL
                     LGGEGFFQIELSGTGVIVLECVVPQSEIVEYELKNGEELKVDGNFAIARTSGVNFSVT
                     KSDKSLIGSALNGEGFLNTFTGSGTVWLAPTQVMYEKLSFGAMPTNDNMNNKVSKQK"
     gene            2067..2531
                     /locus_tag="ACOIAF_003606"
     CDS             2067..2531
                     /locus_tag="ACOIAF_003606"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGZ2670586.1"
                     /translation="MNLNKVKFTEEHAIEVTNWKYEGKYSIYNLPPWEEIKKNNFSLA
                     KEDKRKNFISFINDNKELIGFISLLDEGSSVFFGIGMKPNYCGMGIGKEIISLGLQEC
                     RNKYSTKPIVLNVRTWNMRAVKCYESQGFKIVETKVQKTHLGDGEFFVMRYQ"
     gene            complement(2798..3442)
                     /locus_tag="ACOIAF_003607"
     CDS             complement(2798..3442)
                     /locus_tag="ACOIAF_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2670587.1"
                     /translation="MFGQSFQNCLVLIKENFKLSTLTNLICSIILLCMIPLIQGVANL
                     DRISTAICLENYVAIIGIVMLVPVFSLEQSKEIDEIVSSKALSQVIVQLIRILMSIIG
                     IVVLIIGFVNVLIYLGCNFPVVEYIIGSFASAMFIGSLGMFFSNLFNNTIIGYMISIG
                     YLILNMMTKGKYVGKFFIMSMSQGSFDEKYWLILGSVVLIIASLLIKPVKRKIR"
     gene            complement(3372..4661)
                     /locus_tag="ACOIAF_003608"
     CDS             complement(3372..4661)
                     /locus_tag="ACOIAF_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2670588.1"
                     /translation="MNLFIKECKNILKSMVYYIFIVVLVFFYISQLGSSVSNDVSKNI
                     PNDGNPLVAPQKVENIEEYYSVHNQYPYGQKESMKTEKVIPNATINLIREYQKNTYTT
                     YPTGVVKYIKLDQNKTKTIEKYIEEITGKSISEINSLWKTDESIKIDYSKVSLERFEE
                     IMQRVDNILGGGSAYAFDKLQRFGVVPVTYDEAMKNYNYTIQKDKVSNGYARIFCDYI
                     GIVLSIFPVFVAVFMSLKDKRSRMREIIYSKQVSSCKIVLSRYFALVFMMILPVLILG
                     MKDLLPLIFFAKDKGISIDNLAFIKYTLWWILPTLMIVTAVGMSITILTDTPVAIIVQ
                     LIWWLYSINSTGLKGDYSTMGLMIRHNSVLNGDIIIQNFNQIMINRIVISLGALVLVL
                     LAIFLYEQKRRGNLDVWAKFSKLFSSNKRKLQIKYFN"
     gene            complement(4658..5530)
                     /locus_tag="ACOIAF_003609"
     CDS             complement(4658..5530)
                     /locus_tag="ACOIAF_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2670589.1"
                     /translation="MEMKIAVSNLSKRFGKKQALDNINLEIDSGMFGLLGRNGAGKTT
                     FMRILATLLSKTEGSIHMCGVPIENTKEIRNMVGYLPQDFSMYPNMTVYKAMDYLAVL
                     SDIPLRKRRKIIAKLLSKVNLTNYQNVKVKSLSGGMNRRLGIAQALIHNPRVLIVDEP
                     TAGLDPEERIRFRNLLCEIANDRIVILSTHIVGDIEATCENVAILNSGKVIYNGSTEA
                     LAKLAEGKVYSIEVDKKDIENIKSRFIVIGMLTHGGKAILRIISDDKPFETAVNCNPT
                     IEDGYMLIMGGDNI"
BASE COUNT         2006 a          773 c          700 g         2074 t
ORIGIN      
        1 ataattttaa catgcccata aaatataata cccacaaatg ataaataata ctcaaaatgt
       61 ctattgaaat aactatacat ccttaaataa cttaacttaa atctaaacat ttcaaaaact
      121 tcttaaaaat atgtaaaaat ttgatgataa agtagcgatt ttttcaaaaa aaatgtaata
      181 tagagttata tcttataata ttttagagat aaattttatc atagtgggga gaaatttgta
      241 tggatttaag caaaaaagta aaaattttag aaaatgcatc agatggcaaa agcgaatttg
      301 agatattaga atttgaaaaa cttgagggag cttctagccc attaatggca atgggattat
      361 attttgctag agaagctggt ttgaaagcta gacaaattaa gataaaatta aataattcat
      421 caataaaaac agaagctggt gcattatact attataaagg aaatatatct tctgaaacta
      481 agattggtgg tgttggaggt ctttttaaaa aaggaatatc aggagcactt actaatgaat
      541 caatgatgaa acctgtatac aaaggtactg gagaagttta tttggaacct tcttttagac
      601 attatttatt tatgagtctt gataatgatt ctataattgt tgataaagga ttattctatt
      661 gttgctctga tacaatagaa gtaaaagcat ttacacaaaa gaacttctca tctgctttac
      721 ttggtggtga aggcttcttc caaatagaat taagtggtac tggtgtaatc gttctggaat
      781 gtgttgtacc tcaaagtgaa attgtagagt atgaattgaa aaatggtgaa gaattaaagg
      841 tagatggtaa ctttgcaata gcaagaacat ctggtgttaa tttttctgtt acaaaatcag
      901 ataaatcttt aattggttct gctttaaatg gtgaaggttt cttaaataca tttactggta
      961 gtggtacagt ttggctagct ccaactcaag taatgtatga aaaactaagt tttggagcaa
     1021 tgcctactaa tgataatatg aataataaag tttctaagca aaaatagttg ttttataaat
     1081 atttattaaa tcattattga ttttataaag tcactataaa ttttattaga ttattataaa
     1141 atcagagtgt ggaaatattt atgtgtattc tttaaataaa aatttccaca ttctgtaaag
     1201 ttattatact tgatatataa aattcaattt aggaacatag tctaacattt ataaacatat
     1261 taaggtaatg atttctcttg gtagttattc tcatatctta ttctttgatt atatttatct
     1321 tattcctagc ccttatgtca tattatattt cttatcttat tatttataat tagcattaac
     1381 ttatctaaaa tcttgtattt ttttaatagt aaagtttaca tctatattaa tttcataaaa
     1441 caatcaaggt agtcaagaga ttcaacttta tttgatttta aaaatttaag tgaatttgtt
     1501 cttatacaca cctttttaaa attatatctg tagaaaatat catttagagc tataattaaa
     1561 ttaacttatt aaattttaga aaatttatta aaaaatataa gttaaattaa tatttttaaa
     1621 gattcttttt tgtaactact agaacactaa tttagtagtt attgtatctc taaagtttaa
     1681 gcatggatag atatacattt gataatttgc ctataattta cctactactt ggattattgg
     1741 agcaaataca atatattaat aataagttcc taaaaccaat aaattaacta atacacataa
     1801 agatattgat gttgattata caactataag aaacggattt aacagaattt aatcaagcta
     1861 agaaattata tatatcgtga tgtttttgaa ttagaaggtg attcaaaatt agtttatagt
     1921 cttctgtgaa tccaataaaa agtacagggt acaagaattg ttagtaaaat ttttaaagat
     1981 tttatggata aatataaata gttttactta ttttctaaaa ctttaaaata ccattaatta
     2041 ccttaaatat tttaatagga ggacatatga atttaaataa agttaaattt actgaagaac
     2101 atgctattga agttactaat tggaaatatg aaggtaaata ttcaatatac aatctacctc
     2161 catgggagga aattaaaaag aataattttt cactagcaaa agaagataaa agaaaaaatt
     2221 ttataagttt tataaatgac aataaagaat tgataggttt tataagctta ttagatgaag
     2281 gaagctctgt attctttggt attggtatga aaccgaatta ttgtggaatg ggaattggta
     2341 aagaaataat tagcctagga ttacaagaat gtagaaataa atatagtact aaaccaattg
     2401 tattaaatgt aagaacctgg aacatgagag ctgtaaaatg ttatgagtct caaggattta
     2461 aaatagttga gactaaggta cagaaaacgc atttaggaga tggagaattt tttgttatga
     2521 ggtatcaata gtttataaca atagtcttat tgttaataca acaattaaaa taaaaaaata
     2581 catatgtgta ggtcatagca ataagtgtct ttgaaatata aatttgagag cttttgataa
     2641 aatttggtat acatatatat tttatactac ggatatataa atctatctac atacataaag
     2701 agattgtaag taggtttgtg tatataaaaa tagatttata tttcataaat gtacctttta
     2761 tttgtattac ctttatttta tctaagctct cattttatta tctaatcttc ctttttactg
     2821 gttttattaa gagagatgca attatcaaca caacacttcc aagtatcagc caatatttct
     2881 catcaaaact accttgactc atagacatta taaaaaattt tccaacatac ttgcctttag
     2941 tcatcatatt taaaattaaa tatcctatag atatcatata acctatgatt gtattattaa
     3001 ataaatttga gaaaaacatt cctaaacttc ctataaacat agctgatgcg aaacttccaa
     3061 taatatactc aactactgga aaattacatc caagatatat taaaacattt acaaaaccta
     3121 tgattaatac cactatacct ataattgaca tcagtatcct aattaattgt acaataactt
     3181 gagataatgc ttttgaagat actatctcat caatttcttt tgattgctct agcgaaaata
     3241 cgggtaccaa cattactatc cctattattg caacataatt ttctaaacat atagctgttg
     3301 aaattctgtc taaattagct actccttgta ttagaggaat catacatagc aatataattg
     3361 aacatattaa attagttaaa gtacttaatt tgaagttttc ttttattaga actaaacagt
     3421 tttgaaaact ttgcccaaac atcaagattc ccccttcttt tttgctcata taagaatata
     3481 gctaacaaaa caagcaccaa tgctcccaag cttatcacaa ttctattaat cataatttga
     3541 ttgaagtttt gtataataat atccccattt aatacactat tatgtcttat cattagcccc
     3601 attgttgaat aatcaccttt gagtcctgta gaatttatac tgtaaagcca ccaaattaat
     3661 tgcacaataa ttgctactgg tgtatctgtt aatatagtaa tactcattcc tacagctgta
     3721 actatcataa gagttggtag tatccaccat aatgtatatt ttataaatgc taggttatca
     3781 atagatatac ctttatcttt agcaaagaaa atcaaaggaa gtaaatcttt catacctaaa
     3841 attaatacag gcaatatcat cataaagaca agtgcaaaat atcttgataa aactattttg
     3901 catgaagata cttgttttga ataaataatc tctctcattc ttgaacgctt atctttaagt
     3961 gacataaaaa ctgccacaaa tactggaaat atacttaaaa ctatacctat ataatcgcaa
     4021 aaaattcttg catacccatt actaacttta tctttttgta ttgtatagtt gtaattcttc
     4081 atagcttcat cataggtaac tggaactact ccaaatcttt gtaacttgtc aaaagcatat
     4141 gcagaccctc ctcctaaaat gttgtctact ctttgcatta tttcttcaaa tctttcaaga
     4201 gatactttag aataatctat ttttatactt tcatcagttt tccaaagtga atttatttcg
     4261 ctaatacttt taccagttat ttcttctata tatttttcta tggtcttcgt tttattttgg
     4321 tctaatttta tatacttaac tacccctgtt ggatacgttg tatatgtgtt tttttgatat
     4381 tctctaatta ggtttatagt tgcatttgga atgacttttt ctgttttcat agattctttt
     4441 tgaccatatg gatattgatt gtgtacagag taatattcct caatattctc tactttttgt
     4501 ggagcaacta atggatttcc atcattagga atatttttac ttacatcatt tgaaactgaa
     4561 cttccaagtt gagaaatata gaaaaaaact aaaactacaa taaaaatata atataccata
     4621 ctctttaata tatttttaca ttcttttata aatagattca tatattatct cctcccatta
     4681 taagcatata gccatcttca atagttggat tacaatttac tgctgtctca aaaggtttat
     4741 catctgatat aatcctaagt atagcctttc caccatgagt caacattcct ataactataa
     4801 atctactttt tatattttca atgtcttttt tatctacttc aatactatat actttccctt
     4861 cagctagttt tgctaatgct tctgttgagc cattgtatat gacttttcca ctattcaata
     4921 tagctacgtt ttcacatgta gcttctatat ctccaacaat atgtgtagat aaaataacga
     4981 ttctatcatt tgcaatttcg catagtaaat ttctaaatct tatacgttct tctgggtcaa
     5041 gacctgctgt cggctcatct actattaaaa ctctgggatt atgaattaat gcttgtgcta
     5101 tcccaagcct tctattcata cctccagata aagattttac tttcacattt tgataattag
     5161 taaggtttac tttgcttaat aactttgcta taatttttct tctttttctt agtggtatat
     5221 ccgataaaac tgctaaataa tccattgctt tatatactgt catgttagga tacattgaaa
     5281 aatcttgtgg caaataacca accatatttc taatttcctt agtattctca ataggaactc
     5341 cacacatatg tattgaacct tctgtttttg ataataaagt ggctaaaatt ctcataaatg
     5401 tggtttttcc tgctccattt cttcctaaaa gaccaaacat accactgtcg atttcaagat
     5461 ttatattatc aagtgcttgt tttttcccaa atcttttgct aagattggat actgctattt
     5521 tcatttccat atataaatac acccccagaa aca
//
LOCUS       DBGJFF010000191         5441 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.196, whole genome shotgun sequence.
ACCESSION   DBGJFF010000191 DBGJFF010000000
VERSION     DBGJFF010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5441
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.196"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(259..975)
                     /gene="dapD"
                     /locus_tag="ACOIAF_003610"
     CDS             complement(259..975)
                     /gene="dapD"
                     /locus_tag="ACOIAF_003610"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HGZ2670590.1"
                     /translation="MVNLNDAYEIARYIKEVKKKTPVKVYVNTNSLHFQSTDKFKVFG
                     SNGSYIFVGDYEDIMEVLDKHGNDITDTHVEYDRRNSAIPLKNTLSEHARIEPGAIIR
                     DMVTIEKNAVVMMGAVINIGAVIGEGSMVDMNAVIGARGTLGKNVHLGAGAVVAGVLE
                     PPSATPVIVEDDVLIGANAVILEGVRIGKGAVVAAGAVVTTDVEAGAVVAGSPAKVIK
                     MKDEKTADKTKLMEDLRNLD"
     gene            complement(1235..1984)
                     /gene="dapB"
                     /locus_tag="ACOIAF_003611"
     CDS             complement(1235..1984)
                     /gene="dapB"
                     /locus_tag="ACOIAF_003611"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HGZ2670591.1"
                     /translation="MLKVIATGYDGKMGKILADTIREDNELELVCVAARGLDSYEGDL
                     KIYEDMSTIVEEADVVIDFSHHSNLDNILSYVLKTKTPLVIATTGYNDDEMNKIREAA
                     KVIPVFQSYNMSLGVNVLVKLVKDAAKLLEGFDIEIIEKHHNRKVDAPSGTAVMIANA
                     IKEVLPNVENNYGRYGRSAKRQPNEVGIHAIRGGNIVGEHDALFAGDNEVLTISHQAQ
                     SRGIFASGSIAAAKYLVKQKPGFYNMDSMLG"
     gene            complement(2051..2938)
                     /gene="dapA"
                     /locus_tag="ACOIAF_003612"
     CDS             complement(2051..2938)
                     /gene="dapA"
                     /locus_tag="ACOIAF_003612"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HGZ2670592.1"
                     /translation="MIFKGSAVALVTPFTKDNKVDFDKLGELVEYQIANGTDAIVSCG
                     TTGEANTMTDEEQLATIKYVVEKVNKRVPVIAGSGSNDTMHSVNLSQEAEKLGVDALL
                     IITPYYNKANKAGLKRHFETIANSVKLPIILYNVPGRTCVNISPSLIVELAKIDNIVA
                     VKEASGDLGQVAEIASLVPDDFAIYSGNDDTILPLLSLGGQGVISVLANVCPQETHDL
                     VAKFFEGDIEGSRKLQLGMDALIAALFIEVNPIPVKTAMNLLGFNVGDLRLPLAEMDP
                     ANLEVLKKELVNFGLKVNA"
     gene            complement(3055..4056)
                     /locus_tag="ACOIAF_003613"
     CDS             complement(3055..4056)
                     /locus_tag="ACOIAF_003613"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HGZ2670593.1"
                     /translation="MKKVNVAIVGATGMVGRTFLKVLEERNFPIENLFLFSSSKSAGS
                     KVMFCGKEYIVEELKETSFTDRGIQIALFSAGGSISEKYAPIAASNGILVVDNSSQWR
                     MNPDVPLVVPEVNPEAIKNHKGIIANPNCSTIQAMVPLKALDDKYKIKRVVYSTYQAV
                     SGSGVKGVEDLEKGMNGNPTGFYPHQISYNCLPHIDSFTENGYTKEEMKMVNETMKIL
                     DNYDLKITATTVRVPVKNGHSESINVEFNNSFELDDLVKTLEEAPGVVVVDNPAKNEY
                     PTAVEFDDRDEVFVGRIRRDFSVDNGVNLWVVADNIRKGAATNAIQIAEMALSYDLV"
     regulatory      complement(4229..4403)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            complement(4481..5362)
                     /gene="dapA"
                     /locus_tag="ACOIAF_003614"
     CDS             complement(4481..5362)
                     /gene="dapA"
                     /locus_tag="ACOIAF_003614"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HGZ2670594.1"
                     /translation="MLFKGSAVALVTPFTEDNNVNFEKLGELIEYHIENGTDALVVCG
                     TTGEATTMSESEIFAVIKYTVEKVNKRIPVIAGTGSNNTMLSVHMSQEAEKLGVDGLL
                     IITPYYNKTNEKGLKLHFETIANSVKLPIILYNVPGRTKVNIKPSVVAELAKIDNIVA
                     VKEASGDLAQVAEIAKLVPKDFAIYSGNDDTILPLLSLGGSGVISVLANICPKETHDL
                     VTKFFEGDIEGSKKLQLDMDALIAALFIEVNPVPVKTAMNILGFNVGDLRLPLAEMEE
                     TNLNVLKQELTNFGFKF"
BASE COUNT         1796 a          976 c          660 g         2009 t
ORIGIN      
        1 aattataaaa agtttaatag tttattacat tgatatattg aaaaattgaa ggaaataaaa
       61 atacttgtca gcaagaaata aaaatgatat aagaatgaat aagagaattg taaaaaaata
      121 aaatgattat aaataaaaga taaaaatatt gtaaaaaaga gggtgcctct ggtgaattaa
      181 attcacacag agacaccttt ttaattaatt ttttaaatta gtatattatt gtttaattaa
      241 tagcatagta ctatttaatt aatctaaatt tctaaggtct tccataagct tagttttgtc
      301 agccgttttt tcatctttca tttttataac tttagcagga gaaccagcaa caacggcacc
      361 agcttctaca tcagtagtta caacagcacc agctgcaaca acagcacctt taccgattct
      421 aacaccttca agaataactg cattagctcc tattaaaaca tcatcttcaa ctataacagg
      481 agtagcagaa ggtggctcta aaacaccagc aacaacagca ccagcaccta agtgaacatt
      541 tttaccaaga gttcctctag ctccaataac agcattcata tctaccatag aaccttcacc
      601 aataacagct cctatattta taacagctcc catcattaca acagcatttt tttctatagt
      661 aaccatgtct cttattattg cacctggctc aattcttgca tgttcactta aagtattttt
      721 tagaggaata gcagaatttc ttctatcata ttctacatga gtatctgtta tatcatttcc
      781 atgtttatct aatacttcca ttatatcctc atagtcacct acaaatatat aagaaccatt
      841 acttccaaat accttaaatt tatcagttga ttggaagtgt aaagagttag tatttacata
      901 taccttaaca ggtgttttct ttttaacttc ttttatatac cttgctattt cataagcatc
      961 gtttaaattt accatttgtt aattctcctt gtatttcatt atatttcttt tattatatac
     1021 ttatttgtca attttagtat atacttactt cttattattt atattttatt tagtttttat
     1081 tgtatagaca tagtattaaa taaatttatt atatatgttt gatttgtatt gcgtattatt
     1141 atatctatat tgcatatgtt tgatttgtat tgcgtattat tatatctata ttgcatatgt
     1201 ttaatttata ttgtgtatta ttatatttat actattatcc caacatgctg tccatattat
     1261 aaaatcctgg tttttgctta actaagtatt tggcagcagc tatagaacca cttgcaaaga
     1321 taccacgaga ttgagcttga tggcttattg taagaacctc attgtctcca gcaaatagag
     1381 catcatgttc gccaacaata tttccacctc ttatagcatg aataccaact tcatttggtt
     1441 gtctttttgc actacgacca tatctaccat aattattttc aacgtttggc aaaacctctt
     1501 ttatagcgtt agcaatcata acagctgtgc cacttggggc atctactttt ctgttgtgat
     1561 gtttttctat tatttctata tcaaatcctt ctagtaattt agctgcatct ttaaccaatt
     1621 ttactaatac atttacacca agagacatat tatatgattg gaatacagga ataactttag
     1681 ccgcttcacg tattttgttc atttcgtcat cattatatcc agtagtagca attactaaag
     1741 gtgttttagt ctttaaaacg taagataaga tattatctaa attagagtga tgagaaaaat
     1801 ctataactac atcagcttcc tcaactatag tactcatatc ttcataaatt ttaaggtcgc
     1861 cttcatagct atccagacct ctagctgcaa cacaaaccaa ttctaattca ttatcttctc
     1921 ttatagtatc ggctaaaatc ttacccattt ttccatcata accagttgct ataactttta
     1981 acataatttc ctcctatgat taagggctaa cttttgacca aacttacttt ttaccatgca
     2041 ttaatcttta ctatgcatta acttttagcc caaagttaac taattctttc tttaatactt
     2101 ctaaatttgc tgggtccatt tctgctagag gtaatcttaa atctccaaca ttgaatccca
     2161 ataaattcat tgcagtctta actggtatag gatttacttc tataaataaa gctgcgatta
     2221 gagcatccat accaagttgt aattttctag aaccttcaat atcgccttca aagaatttag
     2281 ctactaaatc gtgagtttct tgagggcaaa cattagcaag tactgatata actccttgac
     2341 ctcctaaaga taataaagga agtatagtat catcattacc agagtagata gcaaagtcat
     2401 ctggaactaa actagctatt tctgcaacct gtcctaggtc tccactagct tcttttacag
     2461 caactatgtt atcaatttta gcaagttcaa ctataaggct aggacttata tttacacaag
     2521 ttcttccagg gacattgtaa agtataattg gaagtttaac actgttagct attgtttcaa
     2581 aatgacgctt aagtccagct ttattagctt tattgtaata aggtgttatt attaatagag
     2641 catctactcc taatttttca gcttcttgac ttaagttaac tgagtgcata gtatcattag
     2701 aaccacttcc agctataaca ggaactcttt tattaacttt ttcaacaaca tacttaatag
     2761 tagcaagttg ttcttcatca gtcatagtat tagcttcacc agtagtacca caagatacta
     2821 tagcatcagt accattagct atttggtatt caactaattc acctaatttg tcaaaatcaa
     2881 ctttgttatc ctttgtaaat ggtgtaacta gagcaacagc agaaccttta aatatcatat
     2941 aaaatcaatc ccctcttttt tatttttgtg ttttatatta cagttacttc taatgcttgt
     3001 ttttgagtta ctattaatat tttacaaaat tatatattta tacaagtttg tatattatac
     3061 taaatcgtat gataaagcca tttcagctat ttgaattgcg ttagttgcag caccttttct
     3121 tatattatca gcaacaaccc aaagatttac accattatca acactaaaat ctcttcttat
     3181 tcttccaaca aatacttcat ctctatcatc aaattctaca gcagttggat attcgttttt
     3241 agcaggatta tcaactacta caacacctgg agcttcttca agtgttttta ctaaatcatc
     3301 taactcaaat gaattgttaa actcaacatt tatagattca ctatgaccat tttttactgg
     3361 aactcttaca gtagttgcag ttatttttaa atcgtagtta tcaagtattt tcatagtttc
     3421 atttaccatt ttcatttctt ctttagtgta tccattttca gtgaatgaat ctatatgagg
     3481 aagacagttg taagagattt gatgagggta gaatccagta ggattaccgt tcattccttt
     3541 ctctaaatct tcaacacctt taacaccaga accagataca gcttggtaag tagagtaaac
     3601 aactctctta attttgtatt tatcatcaag agctttaagg ggaaccatag cttgtatagt
     3661 agagcagtta ggattagcta ttataccctt atgatttttt atggcttctg gattaacctc
     3721 aggaactact aaaggcacat ctggattcat tctccattga ctagagttat ctactactaa
     3781 gattccattt gaagcagcta ttggagcata cttttcacta atacttccac cagcagagaa
     3841 taatgctatt tgtatgcctc tatcagtaaa tgaagtttcc tttaattctt ctactatata
     3901 ttctttacca caaaacatta ctttagaacc agctgattta gaagaagaga ataaaaatag
     3961 attttctatt ggaaaatttc tttcttctaa tacttttaaa aatgttctac ctaccattcc
     4021 agtagcacca actattgcta catttacttt tttcatataa attcacccca attttaaatt
     4081 attaaaacat tattttattt tagttataca agtaagatat gttaatattt ttatatatcc
     4141 aaaaacttta atattatatt aacaacttta attatataca aaaaacgtac aaaggtatac
     4201 tttgtacgtc taatttatca ctcaaagtaa accctgtagc tcatcaccta caaaaataag
     4261 tgacagtatt acacatgtta tgtgcaatcc cagaaaatat tattgcaaga aatattttct
     4321 tcggctgtaa atcctttttc caatcattaa cacctgccag ctactattgg atactcttag
     4381 ttacagctac ctctacccta ggttaatagg atattaaaat ttgattattc taataataat
     4441 acatatttaa gaaaaaatca acttatttat ataaatattg ctaaaattta aatccaaaat
     4501 ttgtcaattc ttgttttaac acattaagat tagtttcttc catttcagct agaggaagtc
     4561 ttaaatcacc aacattaaaa cctaaaatgt tcatggcagt tttaacagga actggattta
     4621 cttctataaa taaggcagca attaaagcat ccatatcaag ttgtaatttt tttgagcctt
     4681 caatatcacc ttcaaagaat ttagttacta aatcatgtgt ttctttagga catatgttag
     4741 caagtacaga gataacacca cttccaccta atgataatag tggtagtata gtatcatcat
     4801 taccggagta tatagcaaag tctttaggta ctaatttagc tatttctgca acttgagcta
     4861 agtctccact tgcttccttc acagcaacta tattatctat tttagcaagt tcagccacaa
     4921 cagatggctt tatattcact ttagttcttc ctggtacgtt gtataaaata attggcaact
     4981 taacgctatt tgctattgtt tcaaagtgaa gtttaagacc tttctcatta gtcttattat
     5041 agtaaggagt tattattaaa agaccatcca cccctaattt ttcagcttct tgactcatat
     5101 gaacagacaa catagtattg tttgaaccag tacctgctat aacaggtatt ctcttattaa
     5161 ctttttcaac tgtatatttt attacagcaa aaatttcaga ttcactcata gtagtcgcct
     5221 cacctgttgt tccacaaaca actagtgcat ctgtgccatt ctcaatgtga tattcgatta
     5281 attcacctaa tttttcaaag ttgacattat tatcctctgt aaatggggta actaaagcaa
     5341 cagcagaacc tttaaataac atataatcaa atctcctttt ttatctaaat ttaatttttt
     5401 aagtacaata ataattatag tacaaattaa ttaattttaa t
//
LOCUS       DBGJFF010000192         5404 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.197, whole genome shotgun sequence.
ACCESSION   DBGJFF010000192 DBGJFF010000000
VERSION     DBGJFF010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5404
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.197"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            344..1033
                     /locus_tag="ACOIAF_003615"
     CDS             344..1033
                     /locus_tag="ACOIAF_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670595.1"
                     /translation="MKVKRNINRVAIIVALNLSLALMVGCMSLVSDKNLSSSEQISKD
                     SLKEQVEDKKDESIELFNNYSQKINDAKGREEVKKDLSQRFSKAIIDSGYSITDSKSD
                     GSFVVSINGAEKPPEKNIRSLFYSMSEDDKEGKVTIDILCAKEYPEDDKLSESDKYIK
                     FIYNLFKSLTNTNLTEKEIFSMVEEEFNKGVGVVELPYMKDIHVEVNKVKQSTKVLKL
                     SLVYKFDISNH"
     gene            1254..3626
                     /locus_tag="ACOIAF_003616"
     CDS             1254..3626
                     /locus_tag="ACOIAF_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase/glycerol dehydratase
                     family glycyl radical enzyme"
                     /protein_id="HGZ2670596.1"
                     /translation="MSQTNRIEAFRQEYINSKPMICCERARIFTESHKQTEGEAICIR
                     RAKAFLETCKELPIKIFENELIVGTAGKFRRTGILTPEFSWQWVDKEMDTFDKRTQDP
                     YVISKEQIEFIRKEIFPYWKGKSLEEVFLARIPEDTAKILVDTGIIDNDSKWRQAVGE
                     VTPDYQDILFVKGYKGIKEDADKKIKELDISVSENIEKIDFYKSVSIVAQGIMTLAQR
                     YSNLAKEMSKQETDEKRKLELIKISEICMNVPANPPTNFYEAIQFVWFVQLGGILSEN
                     PLALNLGRFDQYMYPYYENDAREGKITESEAQELIEALWIKLSEWVWTISANTANYFA
                     GYNQFQNLTVGGKKRNGTDGTNDISYMCLKATESVKTHQPGLSVRVSQGAPDNFVMAV
                     AKLVKQGTGFPAIHSDSAGAQMLLQDGYDAEDARDWSNCGCVVPHFRKTGQWTSAVNI
                     NFAAALEYAMNEGKSRLTGEKMGLDTKNITEFTSFEELKDEFLKQLAYLVKSSVIGTT
                     VAQQIHKEMVPRPFLSTCVDGCLDKGVDLSKGGAKYNIGPVLTGIGLGVVSNSLAAIK
                     KLVFEDKVTTLEELTKALNNDWEGYEELRKLALDVPKYGNDNDYVDSLAIEVSDFYYT
                     ETRKYKDIFGSKFNSAFMGISNYVPTGKIVGATPCGRKATKPLTEGVSPFVGTDTTSP
                     LAAMKSASKINHDVHTGGTLLNLRLNQDLVETERGLRNLTSMIKSYFALGGFHVQFNT
                     ISNDTLLKAQENPEEYKDLLVRVAGYSTQFVNLSREMQDAIIARNSHSNF"
     gene            3641..4543
                     /locus_tag="ACOIAF_003617"
     CDS             3641..4543
                     /locus_tag="ACOIAF_003617"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HGZ2670597.1"
                     /translation="MSKKGRVVKVQHFSVNDGDGIRTTIFLEGCKLKCKWCSNPDSWS
                     NIVKLGVMKDKCVSCNRCIDVCPQNISSLFDRAQINNKCDLCGECIKVCLKDAICIMT
                     EEMSVEEIVEEVEKDFIFFFESNGGITFSGGEPTLQIDFLRELVDIFYDKGINIAIET
                     CGYFDWNKVNDVFEKIDHIFVDIKSMDDNIHKEYTGVSNKIILDNICRLSKLNKSMVI
                     RVPIIYGVNDSEENIRNTALFVKQNVPGGKMELLPYHKFGIDKYKALGLEDYIYEFDE
                     ICNNHMLKLKEIVELTGVKIIEYK"
     gene            4563..5114
                     /locus_tag="ACOIAF_003618"
     CDS             4563..5114
                     /locus_tag="ACOIAF_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGZ2670598.1"
                     /translation="MDNKFPNVGENLRLLRQEMGISLDKASKMTGVSKAMLGQIERGE
                     SSPTVSTLWKISSGFKINFTTLLNENTNTYEVIKKEEVEPIVEQKGNMKLYPIYPFSP
                     QRRFELFIIELEENCTHVSSTHSHVLEEYVLVIEGKLDLNVDGKTYILESGHSIRFDG
                     TLEHIYKNLNKGKTIFHNIEVYR"
BASE COUNT         2161 a          546 c         1015 g         1682 t
ORIGIN      
        1 acaaaaatat aatggagaat tattatttga aaatagtatc gataaattta tactaaatat
       61 atatatacca ttggaccaag atactgacag ttggggcgat taagttacca gttgggacat
      121 atgtattgat acaaaaaaat aaggatgtta tcttttaata acaaaagata acatccttat
      181 ttctgtatta aggagcttat aattttacta atagtaagtt ataagaattg gataggaagg
      241 tgatatgtat tgaataataa atctttaata taaagaaaat ataaatatat tctagtgcaa
      301 tgaataatac tttagagcgg ttagtgtgat gggaggattg tgtatgaaag taaaaaggaa
      361 tataaataga gtggcaataa ttgtggcttt aaatcttagc ttagcattga tggtaggatg
      421 tatgtctctt gtatctgata agaacttaag tagctcggaa caaattagta aagattcatt
      481 gaaagaacaa gttgaagata aaaaagatga atctatcgag ctatttaata attactcaca
      541 aaagattaat gatgcgaaag gtagagaaga agtaaaaaaa gatttatctc aaagattttc
      601 gaaagctata atagacagtg gctatagtat tactgactca aaatcagatg gtagctttgt
      661 agtatctata aatggtgctg aaaaaccacc tgaaaaaaat attcgaagtt tattctattc
      721 tatgtctgaa gacgataaag aaggaaaagt aacaatagat attctctgtg ctaaagagta
      781 tccagaagat gataagttat ctgagagtga taaatatatt aaatttatat acaatttatt
      841 taaatcattg actaatacga acttaactga gaaagaaatt ttttctatgg ttgaagaaga
      901 atttaataag ggagtaggtg ttgtggagct tccttatatg aaggatatac atgttgaagt
      961 taacaaagtt aaacaatcaa ctaaggtttt aaaactaagt ctagtataca aatttgatat
     1021 ttcaaatcat tagtatatta gaaataacta taacaatata gaaaatatat catagaaaat
     1081 ttttttagat tttataaatt taacaaaaaa agtattaata tataggtgtt caaggtagat
     1141 gaaaaaaaat aagttatttt gcttccattt tatgctaaag gacagtataa tataattaaa
     1201 gtatgttata acatacaaaa atgtagtata tattatccaa agggggattt taaatgagtc
     1261 aaactaatag aatagaagca tttagacaag aatatattaa ttctaaacct atgatatgtt
     1321 gtgaaagggc tagaattttt acagaatcac ataaacaaac tgagggagaa gctatctgta
     1381 taagaagagc taaagctttt ttggagacat gtaaagaact tcctataaaa atctttgaaa
     1441 atgaattaat agtaggtaca gcaggaaaat ttagaagaac agggatattg acaccagagt
     1501 tttcttggca atgggtagac aaagaaatgg atacttttga taaaagaact caagacccat
     1561 atgtaatatc aaaagaacaa atagaattta taagaaaaga aatatttcca tattggaagg
     1621 gaaaatcttt ggaagaagtt ttcttagcaa gaatcccaga agatacagct aagatattag
     1681 tagatacagg aataatagat aatgattcta agtggagaca ggcagttggt gaagtaacac
     1741 cagattatca agatatattg tttgtaaaag gatataaagg gattaaggaa gatgcagata
     1801 aaaaaattaa agaattagat atatcagttt cagaaaatat tgaaaagata gatttttaca
     1861 aatctgtttc tatagttgca cagggtatta tgacattggc acaaagatat tcaaatcttg
     1921 caaaagagat gagtaagcaa gaaacagatg aaaagagaaa gttagaactt ataaaaattt
     1981 cagagatatg tatgaatgta ccagcaaacc ctccaactaa tttttatgaa gcaatacaat
     2041 ttgtatggtt tgttcaatta ggtggtattc tatcagaaaa tccattggca ttaaatttag
     2101 gtagatttga ccaatatatg tatccatatt atgaaaatga tgctagagaa ggtaaaataa
     2161 ctgaatcaga agctcaagaa ttgatagaag cactttggat taagttatca gaatgggtat
     2221 ggactatatc agcaaataca gcaaattact ttgcaggata taatcaattc caaaatctta
     2281 ctgttggtgg taaaaagaga aatggtactg atggaacgaa tgatatctca tatatgtgtt
     2341 taaaggctac agaaagtgta aaaactcacc agccaggatt aagtgttaga gtgagtcaag
     2401 gagcaccaga taatttcgta atggcagttg ctaaattggt aaaacaagga actggtttcc
     2461 ctgctataca cagcgatagt gcaggggcac aaatgttatt acaagatgga tatgatgcag
     2521 aagatgctag agattggagt aactgtggtt gtgtagttcc tcattttaga aaaacaggac
     2581 agtggacttc agccgtaaat atcaattttg cagcagcttt agaatatgct atgaatgaag
     2641 gtaagagtag attaactgga gaaaaaatgg ggttagatac aaaaaatatc acagaattta
     2701 cttcttttga agaacttaag gatgagtttt taaagcaatt agcatatctt gtaaaaagtt
     2761 ctgttatagg aactactgtt gctcaacaaa ttcataaaga aatggtacca agaccatttt
     2821 tatctacttg tgtagatgga tgtttggata agggagttga tttaagtaaa ggaggagcaa
     2881 aatataatat aggtcctgta ttaactggta tagggttagg tgtagtttca aattcattgg
     2941 cagctataaa aaaattagta tttgaggata aggtgactac gttagaagaa ttaacaaaag
     3001 cactaaataa tgattgggaa ggttatgaag aattaagaaa acttgcgttg gatgttccta
     3061 agtatggaaa tgataatgat tatgtagatt cattagcaat tgaagtttct gatttttatt
     3121 atactgaaac tagaaaatac aaggatattt ttggctctaa atttaatagt gcatttatgg
     3181 gtatatcaaa ctatgtacca actggaaaaa tcgtaggtgc aactccttgt ggaagaaaag
     3241 cgacaaaacc tttgacagag ggagtttctc catttgttgg tactgataca acaagtccac
     3301 tagcagccat gaaatctgca tcaaaaataa atcatgatgt tcatactggt ggaacacttc
     3361 taaatttaag actgaatcaa gatttggtgg aaactgaaag aggacttaga aatctaacat
     3421 ctatgattaa atcatatttt gctttaggag gattccatgt acaatttaat acgatatcaa
     3481 atgatactct attaaaagct caagaaaatc cagaagaata taaagattta ttagtgagag
     3541 ttgctggata tagtactcaa tttgttaatt tatcaagaga aatgcaagat gctataatag
     3601 ctagaaattc acatagcaat ttttaattat gaggtagatt atgagtaaaa aaggtagagt
     3661 tgtaaaagta caacattttt cagtaaatga tggagatgga ataaggacta ctatattttt
     3721 agaaggatgt aaattaaaat gtaagtggtg ttctaatcca gattcctgga gtaacatagt
     3781 aaaattaggt gttatgaaag ataaatgtgt atcctgtaat agatgtatag atgtttgtcc
     3841 tcaaaatata agttcactat ttgatagagc gcaaataaat aataaatgtg atttatgtgg
     3901 agagtgtatc aaggtatgtc ttaaagatgc tatttgtata atgaccgaag aaatgagtgt
     3961 tgaagaaata gtagaggaag ttgaaaaaga ttttatattt ttctttgaat ctaatggagg
     4021 gataactttt tctggaggtg aacctacact tcagattgat tttttaagag aattagttga
     4081 tattttttat gataaaggca taaatattgc aattgaaact tgtggatatt ttgattggaa
     4141 taaggtaaat gatgtatttg agaaaataga ccacatattt gtagatataa aatctatgga
     4201 tgataatatt cataaagaat atacaggagt aagtaataaa attatattag ataatatatg
     4261 taggctttca aagttgaata aatctatggt tataagagtg ccaattattt atggagtaaa
     4321 tgatagtgaa gaaaatataa gaaatactgc attatttgtc aaacaaaatg ttccaggagg
     4381 caagatggag ttacttccat atcataaatt tggaatcgat aaatataagg cattagggct
     4441 tgaagattat atatatgaat ttgatgaaat ttgtaacaat catatgttaa aattaaaaga
     4501 gatagtagaa cttacaggag taaaaatcat agaatacaaa tagctatcag gaggatttta
     4561 acatggataa taaatttcct aatgtgggag aaaatttaag gttattgaga caagaaatgg
     4621 gcataagttt agacaaagct tcaaagatga caggtgtcag taaagcaatg cttggacaga
     4681 ttgagagagg tgaatcaagt ccgacagtat ctactttatg gaaaatttca tctggattta
     4741 aaataaattt tactacacta ttaaatgaaa atacaaatac ctatgaggtt ataaaaaaag
     4801 aagaagtaga gcctattgtt gagcaaaaag ggaacatgaa gttatatcca atataccctt
     4861 ttagccctca aagaaggttt gaacttttta taattgagct tgaggaaaac tgtactcatg
     4921 tttcatctac acatagtcat gttttagaag agtatgtact cgtaatagaa ggtaaacttg
     4981 accttaatgt ggatgggaaa acttatattt tagagtctgg acattctatt aggtttgatg
     5041 gaactttaga gcatatatat aaaaatttaa ataaaggaaa aactatattt cacaatatag
     5101 aggtttatag ataattttat agttatcctt ttctaaaata aatgaaaatt ttagaaaagg
     5161 ataacttatt tttataatgt cgaaaagatt tatatgtatg tgatgatgtt attgttttaa
     5221 aaaaaacata aaagcattat aataatatta acaaaataga ataaagagga agagaatatg
     5281 attataaagg tttcaatgac agtattagta ttaatattat tgtatattct gtataagtat
     5341 gaaactcagc atatagaaac gacagaatat actattttca acaaaaaaat tccaaaggaa
     5401 tttg
//
LOCUS       DBGJFF010000193         5354 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.198, whole genome shotgun sequence.
ACCESSION   DBGJFF010000193 DBGJFF010000000
VERSION     DBGJFF010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5354
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.198"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            142..873
                     /locus_tag="ACOIAF_003619"
     CDS             142..873
                     /locus_tag="ACOIAF_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HGZ2670599.1"
                     /translation="MNIYAHRGFSGKYPENTILAFKKCLDMDIYGIELDVHRTKDGKI
                     VVIHDEKVDRTFNGHGFVKDFTLRKLKTLNSSFEGYQSNKECKIPTLEEVLILISPTD
                     LILNIELKTDKINYPNIEKDVLELILKYNMKNRVLISSFNSNSLKNFHKLDPSIKTGL
                     LCYLPINNVVNFAKFLGNSYLHPPLVLVNESLIELCHKNLLGVNVYTVNEEDDILHCL
                     KLNVDGIFTNYPDIASNLLHSKQYS"
     gene            953..1588
                     /locus_tag="ACOIAF_003620"
     CDS             953..1588
                     /locus_tag="ACOIAF_003620"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HGZ2670600.1"
                     /translation="MDNLLNELEALKSNLDNLPIEKIENYDLSKTALFIIDVNNGFAR
                     QGALYSPRVESLIKPIEMFTKKISNKLNKVIAFTDSHTPKSIELLSYPVHCLENDVES
                     ELVDELKSIENLQILPKNSTNGFFALENLDFDNIDNIIIVGDCTDICIYQFAITLKSY
                     FNQHNIEKNIVVPMNLVDTYDIPNVHPAEILNLVFFNSMIQNGVNVLKEIR"
     gene            complement(1839..2432)
                     /locus_tag="ACOIAF_003621"
     CDS             complement(1839..2432)
                     /locus_tag="ACOIAF_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3867 domain-containing protein"
                     /protein_id="HGZ2670601.1"
                     /translation="MSDDRIIDFNELKNKVKDSDVDKFEQYIYNLYFSVMDGKMSMAE
                     FSRKIFDYMRDNNISQEKFMKIQKQFMERYGMDTEEVEKQLRNFGIDPSTAGFMSNNT
                     SSKVSTEDLESFKKSAGFYEKYGEKIQPKSCITTFIKNDLNDINVIIDQEKIMLCSDR
                     KINLMDSELNEFLLEYKNMFNKKIKVVMCETTNKYDY"
     gene            complement(2503..3366)
                     /locus_tag="ACOIAF_003622"
     CDS             complement(2503..3366)
                     /locus_tag="ACOIAF_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HGZ2670602.1"
                     /translation="MLGIIAPSGPLRNTSLEEIKFNLESYGYEVKFSESCSLNYKGYL
                     AGNDDIRARDIEDMFLDKDVDIIMCLRGGYGTTRILDKINYDIIKQNPKPFIGFSDIT
                     GLNLAFYKNCGLLPYHGIMAANVGKWDKFTYKSLVNALEFKDELYLENPKEEKIYTVC
                     EGKAEGIIMGGNLSLIIATMGTKYEIDAKDKILFIEEIGEPQYKLDRMLTQLYSSGKL
                     EECNGIIFGDFKDCIEENDLMELLIEFANKVNKPSIYNLQSGHCIPMITIPLGRMCEL
                     DATEKIVKLKK"
     gene            3529..4698
                     /locus_tag="ACOIAF_003623"
     CDS             3529..4698
                     /locus_tag="ACOIAF_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HGZ2670603.1"
                     /translation="MLNIKTLCHEINDWVINIRRDLHKTPELGLEEFQTKKKIIKYLN
                     EIGINYIEYKNHTGITAYINVSPNFETVAIRADIDALPITEELNYSYKSTNIGKMHAC
                     GHDAHTAILLGTCNILFKLKDYLNVNVKFFFQPAEETIGGAQLMIEDGCLENPNVKYI
                     FGLHVNPNINKNLIELKYNTLNASTDTLQLTVHGSKCHGAYPHQGIDAIVISAHIITA
                     LQTIVSRNTNPTDSVVISLGKIEGGIKENIVCDKVVIRGTLRTLTPETREFSKKRIRE
                     ICDFTCKTFGGSISVEIEEGYPALINSNHLVDYVKQNAVELFGEENIILKDSPTLGAE
                     DFSYFLRHCEGAFYHLGCANREKNITSPLHTSTFDIDEDCLITGVMLHVKNVLSF"
     gene            4799..5275
                     /locus_tag="ACOIAF_003624"
     CDS             4799..5275
                     /locus_tag="ACOIAF_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HGZ2670604.1"
                     /translation="MKEILINPIGKICIQGEEVFIKLDKKYVPALNELDDFSHLNVFW
                     WADGFDDPELRSILETPKPYKSGPDVIGIFATRSPIRPNPIALTAVQIINIDHENGII
                     KIPYIDANDNSPVIDLKPYTPSVDRVENPSVPKWCSHWPKSVEKSGEFNWEDEIEF"
BASE COUNT         1921 a          783 c          672 g         1978 t
ORIGIN      
        1 ttttattttt ctacatttat ataatatata aattacaaat agttatttaa ctaatatata
       61 acaatacctt ttggaatatg atatattata ttaattaaat ttttataaga taagttatac
      121 ttataggagg gaaccatata aatgaatatt tatgctcata gggggtttag tggaaagtat
      181 ccagaaaata ctattctagc ttttaaaaaa tgtttggata tggatatata tggaatagaa
      241 cttgatgtcc acagaactaa ggatggaaaa attgtagtta ttcatgatga aaaggttgat
      301 agaactttta atggacatgg ttttgtaaaa gactttactt taagaaaatt aaaaactcta
      361 aactcatctt ttgaaggata tcaaagcaat aaagaatgta aaattccaac tttagaagaa
      421 gtattaattc taatttcacc tacagacctt attttaaata tagaactaaa aactgacaaa
      481 ataaactatc ccaatataga aaaagacgtt ttagagctta ttttaaagta taatatgaaa
      541 aatagagttc ttatatctag tttcaacagc aatagcttaa aaaactttca taaattagac
      601 cctagtataa aaacaggatt attgtgttat ttacctataa acaatgtagt caattttgct
      661 aaatttctgg gtaatagtta ccttcatcca cctctagttt tagtaaatga atctctaatt
      721 gaactttgtc acaaaaatct acttggtgtc aatgtatata ctgtaaatga agaggatgat
      781 atactacact gtctaaaact caatgtagat ggtattttta ctaactatcc agatattgct
      841 tcaaatcttt tacactcaaa acaatacagc taattataca atagtttgta ctccttgact
      901 tttatataat taatatgtac aattttatta aatttatctt ggaggtataa ctatggataa
      961 cttattaaat gaacttgaag ctttaaagag taatttagat aatctaccaa tagaaaaaat
     1021 agaaaattat gatttgagta aaactgcttt atttataatt gatgttaata atggctttgc
     1081 aagacaaggt gcattatact ctcctagagt agagtcttta ataaagccaa ttgaaatgtt
     1141 tacaaaaaaa atatcaaaca aattaaataa agtaatagca tttactgatt ctcatactcc
     1201 taaatcaata gaacttttga gttatcctgt tcactgtttg gaaaatgatg tagaaagtga
     1261 attggttgat gaacttaagt cgatagaaaa cctacaaatt cttcctaaaa attctactaa
     1321 tggttttttt gcattagaaa atttggattt tgataatata gataatataa ttatagttgg
     1381 cgattgcaca gatatatgta tatatcaatt tgcaataact ctaaaatcat attttaatca
     1441 acataatatt gaaaaaaata tagttgtacc tatgaattta gtagatactt atgatattcc
     1501 taacgttcac cctgctgaga ttttaaactt agtatttttc aacagtatga ttcaaaatgg
     1561 agttaatgta ttaaaagaga ttcgttaagc ctctaatact ttacatccca tataatacaa
     1621 ttatcagata tttattttta ataaaatttc taatagaaaa acacagtttt agtgagaaga
     1681 ttacaattta aaacactatc tattattact aatcttcaca atataaaaaa tcgcagttta
     1741 gatattcctt atacaaattt ataaggaata ataaattgcg atttttattt ttaagtagta
     1801 caattaattt atactacttc agtatttaaa ttcatttttt aataatcata cttattcgta
     1861 gtttcgcaca ttactacctt tattttttta ttaaacatat tcttatattc taataaaaac
     1921 tcatttagtt ctgaatccat aagatttatt tttctatcac tacaaagcat tatcttttct
     1981 tggtcgatta ttacattaat atcatttaag tcatttttta taaatgttgt tatacaactc
     2041 tttggttgga ttttttctcc atatttctca taaaatcctg cactcttctt aaatgactct
     2101 aaatcttctg tagatacttt gctggaagta ttattactca taaaacctgc tgtagaaggg
     2161 tcgattccaa agtttctaag ttgtttttct acttcttcag tgtccatacc atatctctcc
     2221 ataaattgct tttgtatttt cataaatttt tcttgagata tgttgttatc tctcatataa
     2281 tcgaatattt ttcttgaaaa ctcagccata gacatctttc catccataac agaaaaatat
     2341 aagttatata tgtattgctc aaatttatct acatcagaat ctttaacttt gttctttagt
     2401 tcgttaaagt ctattattct atcatcagac atttttcatc ctcctagcta aatttttact
     2461 tgtataagat tataccatat ttctattata tttatataca cactattttt ttaatttaac
     2521 gattttttct gtagcatcta attcgcacat tctgcccaaa ggaatagtta tcattgggat
     2581 acaatgtcct gactgaagat tatatataga aggtttattg accttatttg caaattctat
     2641 tagtaattcc atcaaatcat tttcttcaat acaatcctta aaatctccaa aaattatacc
     2701 attacattcc tcaagttttc ctgaagaata tagttgtgtt aacattctat ccaatttata
     2761 ttgaggttct ccaatttcct caataaacaa aattttatct tttgcatcta tttcatattt
     2821 tgttcccatt gtggcaatta ttaaggataa atttcctccc attattattc cctctgcttt
     2881 accttcacat acagtataaa tcttttcttc ttttggattt tctaaatata attcatcttt
     2941 aaattctaaa gcatttacca atgatttata ggtaaattta tcccatttac ctacatttgc
     3001 tgccattatt ccatgatatg gtaaaagacc acaattttta tagaaagcta aatttaaacc
     3061 tgttatatca gaaaaaccta taaatggttt tggattttgc tttattatat cataatttat
     3121 tttatccaat attcttgttg ttccataacc tcctcttaag cacataatta tatctacatc
     3181 tttatctaaa aacatatctt ctatatccct tgctctaata tcatcattac cagctaaata
     3241 acctttataa tttaaactac aactttcaga aaacttaact tcgtaaccat aactttctaa
     3301 attaaacttt atttcctcca aacttgtatt tcttaatggc ccagatggcg caattattcc
     3361 taacatttaa acacctcttc ttataaaatg atatattctt ctccatacta aatatctcta
     3421 atataattat aagatatttt atttgtactt gatacataat aatgatttac ataaaataat
     3481 ataattaatg aagaaaaata aatttttaat atagttagga tgtgttttat gcttaatata
     3541 aaaactcttt gtcatgagat aaatgattgg gttataaata taagaagaga tttacataag
     3601 actccagaac ttggattaga agaatttcaa actaagaaaa aaattattaa atacttaaat
     3661 gaaataggta taaattatat tgaatataag aatcatacag ggataactgc atacattaac
     3721 gtgagtccaa actttgaaac agttgctata agagctgata tagatgcctt acctataaca
     3781 gaagaactta attattctta taaatctact aatattggca aaatgcatgc atgtggtcat
     3841 gatgcacaca ctgctatttt actgggtact tgtaatatac tatttaaatt aaaggattac
     3901 ttaaatgtaa atgtaaaatt cttttttcaa cctgccgagg aaactattgg tggggctcaa
     3961 cttatgatag aagatggatg tcttgaaaac ccaaatgtaa aatatatatt tggacttcat
     4021 gtaaatccca atatcaacaa aaatctaatt gaattaaaat acaacactct aaatgcttcc
     4081 accgatacac ttcaattaac tgtacatggt tcaaaatgtc atggagctta tccacatcaa
     4141 ggtattgatg ctattgttat atctgctcat attattacag ctctacagac aatcgttagt
     4201 cgaaacacaa atccaactga ttctgtggtt atttctctag gaaagattga aggtggaatc
     4261 aaggaaaata tagtttgtga taaagtggtt ataaggggta ctttgagaac cttgactcct
     4321 gaaactagag agttttcaaa aaaacgaatt agagaaattt gcgattttac ttgtaaaaca
     4381 tttggaggaa gtatttctgt tgaaattgag gaaggttatc cagctcttat caattcaaac
     4441 catctagtag attacgtcaa acaaaatgct gtagaattgt ttggtgaaga aaatattatc
     4501 cttaaagact cgcccactct tggggctgaa gacttttcgt attttctaag acactgtgaa
     4561 ggagcttttt accaccttgg ttgtgcaaat cgtgaaaaaa acattacttc ccctttgcat
     4621 acctctactt ttgatattga cgaagattgc cttattactg gtgtcatgct ccatgtaaaa
     4681 aatgtattat cattctaact attaatattt ttatttatta ttttatattg accctcacat
     4741 gatgtcatgt tataccctaa aattacaaat gataaattaa atagttggag gtaatattat
     4801 gaaagaaata ttaataaatc ctataggtaa aatatgtatt caaggcgaag aggtctttat
     4861 taaattagac aaaaagtatg tacctgcatt aaatgaactc gacgatttca gtcatttaaa
     4921 tgtattttgg tgggctgatg ggtttgatga tcctgaacta agaagtattt tagaaacccc
     4981 aaaaccttat aaaagtggac cagacgttat aggaatattt gcaacaaggt cacctataag
     5041 acccaatcca atagcattga ctgctgtaca aattatcaat atcgaccatg aaaatggcat
     5101 aataaaaata ccatatattg atgctaatga taatagtcct gttattgact taaagccata
     5161 tactccaagc gttgataggg ttgaaaatcc gtccgttcct aaatggtgta gtcactggcc
     5221 taagagtgta gaaaaatcag gagaatttaa ttgggaagat gaaattgaat tttaaattac
     5281 ttttttcttg atttcattaa cttgttttcc acatttattt tatttacact taccaagatt
     5341 ttatgtcaca tata
//
LOCUS       DBGJFF010000194         5340 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.199, whole genome shotgun sequence.
ACCESSION   DBGJFF010000194 DBGJFF010000000
VERSION     DBGJFF010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5340
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.199"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            <1..117
                     /locus_tag="ACOIAF_003625"
     CDS             <1..117
                     /locus_tag="ACOIAF_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HGZ2670605.1"
                     /translation="IIIREWICPSCNETHDRDINASINILKEGLRLITIQNK"
     gene            244..1146
                     /locus_tag="ACOIAF_003626"
     CDS             244..1146
                     /locus_tag="ACOIAF_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HGZ2670606.1"
                     /translation="MLPQDYYGLIVILGVILGVMGAFYNWFTLYVQSLYKKIPKIGTF
                     GKLLIPFIMAGILGIVMPDVLGSGHNLVEELTSHEYIFGMVVLIFVIRFIFSAVSFGS
                     GAPGGIFFPLLVLGAFIGGIFGMVGVKVFGMNPDYVNNFVLLAMAGYFTAIVRAPLTG
                     IILIFEMTGSVSQMLSLSVISIVAYIVATLMKSKPIYESLLERLLEKNGEPVHKERGE
                     KILDQFSVMFGSPVANKAIQDVQWPENCLLVAIQRGGEEIIPRGKTILRPSDVIVTMT
                     DERDSGAVYDKMENLCKEHTSHIF"
     gene            1758..2771
                     /locus_tag="ACOIAF_003627"
     CDS             1758..2771
                     /locus_tag="ACOIAF_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HGZ2670607.1"
                     /translation="MVSSFLNDIFRTILVSCIFIVILLVFRITLFKIFSKKFNYYIWL
                     IVIIKLSLPFMNYTFIFNESKYNGNINKISLGNFNSISIVYSTVLVSVWIAITIFYLV
                     YTLLKYIKLKNMINDLSYDVEDDKIKNLYENLLKELKINKKIRLKYSYEVESPVFFNS
                     CVILPPCDYTLKELNWIFRHELMHFKSKDLYIKYLVIFLKCVYWFNPFVYIMEKFIDL
                     DCEFYCDERVLKNCTIEEKQDYALTIISAMRKGSNSSNKFVAGLHKESDIKKRVFSMF
                     NEKYRNGILIAVILCLLSSITYFKIDLITTKSIFYPLSKQTPIENRTNSKPTYQINVY
                     IDN"
     gene            3123..3692
                     /locus_tag="ACOIAF_003628"
     CDS             3123..3692
                     /locus_tag="ACOIAF_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HGZ2670608.1"
                     /translation="MRKKLEDMTLEELWNLFPIFLTTHKECWKNWFKEEQYRLTGILP
                     MQKIVRISHIGSTSVSTIYAKPIVDILIEIIPDYNMKDIKDLIESNGYICMSESSSRL
                     SLNKGYTVEGFEERVFHLHIRYVGDNDELFFRDYLIDHPDVAKEYEKLKLDLWKKYEH
                     NRDAYTDSKLDFVREFTEKAKVLYSNRYN"
     gene            complement(4028..>5340)
                     /locus_tag="ACOIAF_003629"
     CDS             complement(4028..>5340)
                     /locus_tag="ACOIAF_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HGZ2670609.1"
                     /translation="SINFILILINIFIAFFLYKLTSYFIDFRNHWIFKIILILGYSII
                     SQMIIWLSDPVNILFTLFGYFILLIICSKSKLIPIISVVMILYPIIIGLNFLFINNPI
                     YEELIHSKEYILILSIVQIISALIKLLFWFLIHIFLKNRLVNIKQYLSNKIWIFIDMV
                     CISSFLSILIAIILPPTNLIPMGSNKNIILGNLPYGTYLIILSAILANIGVILLLQPL
                     IENVKMKIEKQANHLKEKYYNLLENQQIQIRKMRHDMNNHFQMIESYLEIEDIAGAKK
                     YFNQLKIGIGTLSGKQFCKNQALNSILNLRHTSLEENGVDVHFNIDIDNHLGIEPIDL
                     CTIFSNSLDNAIEASLKIPDLSNRKVVLKARCEKEYFSYLLTNNKVNPINRKQELFIS
                     DKKDSNSHGYGIENIREIVNKYNGKLDISYTESEFSLFLYIRLF"
BASE COUNT         1966 a          537 c          890 g         1947 t
ORIGIN      
        1 attattataa gagaatggat ttgtcctagt tgtaatgaaa ctcacgatag agatataaat
       61 gcaagtataa atatattaaa agaaggatta agactaataa caattcaaaa taaataactt
      121 atgtaagaac cgtaggaact acggggatag cctgtggaga attagtaaga catattttag
      181 tatgcaaaat tctatgaagc aggaaccctg tgactttagt catgggaggt tcagattgga
      241 aatgtgttgc cacaagatta ttatggatta atagtgattt taggtgttat tcttggagtt
      301 atgggggcat tttacaactg gtttactcta tatgtgcagt ctttatacaa gaagattcct
      361 aaaattggta cctttggaaa actgttgatt ccatttataa tggctggtat tcttgggatt
      421 gttatgcctg atgttctggg gagtggacac aatttagttg aagaacttac aagtcatgaa
      481 tatatttttg gaatggttgt tttgattttt gttatacgtt ttatattttc ggctgttagt
      541 tttgggtcag gtgcaccagg gggaatattt tttccattgc ttgttttagg agcatttata
      601 ggtggaatat ttggtatggt aggtgttaaa gtttttggaa tgaatcccga ttatgtaaat
      661 aactttgtgc ttttggctat ggcaggatat tttacagcta ttgtgcgtgc ccctttgact
      721 ggtatcatat taatttttga aatgacaggt tctgttagtc aaatgctttc actatcagta
      781 atttcaattg tggcatatat tgtggctaca cttatgaagt ctaaacctat ttatgaaagt
      841 cttcttgaga ggttacttga aaaaaatgga gaacctgtac ataaagagag gggcgaaaag
      901 atactagacc agttctctgt tatgtttggc tctcctgttg cgaacaaggc aattcaggat
      961 gtacagtggc ctgaaaattg tcttttggta gcaattcaaa gaggtggaga agaaattatt
     1021 cctagaggta agacaattct tagaccaagt gatgtaattg tgactatgac agatgagaga
     1081 gatagtggtg ctgtgtatga taagatggaa aatttgtgta aagaacatac gtcacatatt
     1141 ttttagagat aatgatgaat gtttaataat gatagaatat ttaatgaaga tttgattaaa
     1201 aaattggtaa tttgaaagag agttaaggaa ttaatataag aaaatgaaaa tgtgatatct
     1261 tagatgtaaa agagtttgaa gaaaataatt gtatatattt gttaatgaga ttatatgaac
     1321 taatggtata tttatactgt gctaaaagtt gtactattgt atgaggaatg ggaataaaga
     1381 gtttgaaaaa ataggtagaa gatttttcag atattcttat tgggatattt taattaatat
     1441 attttaatta cgatattttg ggaagaatag aatttattta taaatgaaaa taattaaaag
     1501 gggttaaggt tgtattagcc ccttttgctt atagagtttg gaaatggtat cttgatagca
     1561 gttgtttcat gcttattata ttttattaca tattttaaaa ttgaccttat tactcctaaa
     1621 tcattgttct atcaactttt aaaacacttt caagacaatc gcctattgaa aacaaagtta
     1681 attaaaaacc tacatatcag tttagtattc gtgtagatga ttatataaat ataaaagtat
     1741 aagaaaggtg agtatctgtg gttagttcat ttttaaatga tatctttaga acaattttag
     1801 taagttgtat ttttatagtt attttattag tctttagaat aaccttattt aagattttca
     1861 gtaaaaaatt taattactat atatggctta ttgtgattat taagctatca ttaccattta
     1921 tgaattatac ttttatattt aatgagtcaa aatataatgg taatattaat aagataagtt
     1981 taggcaattt taatagtatt tctattgtat atagtactgt tttagtctct gtttggattg
     2041 ctattacgat attttattta gtttatactt tattgaaata tattaagctt aaaaatatga
     2101 taaatgattt gtcttatgat gttgaggatg ataagataaa aaatctttat gaaaatttat
     2161 taaaagagct taagataaat aaaaaaatac ggcttaaata ttcatatgaa gttgaaagtc
     2221 cagtattttt taattcgtgt gtcattttac caccatgtga ttatacatta aaagagttaa
     2281 actggatatt taggcatgag ttgatgcatt ttaagagtaa agacctttat attaagtatt
     2341 tggtgatatt tttaaaatgt gtttattggt ttaatccttt tgtgtatata atggagaagt
     2401 ttatagattt ggattgtgaa ttttattgtg atgaaagagt tttgaagaat tgtacgatag
     2461 aagagaagca ggattatgca ttgacaataa ttagtgcaat gagaaaaggt tcaaatagtt
     2521 caaataaatt tgtggctgga ttacataagg agtcagatat taaaaaaaga gtgtttagta
     2581 tgtttaatga aaagtacaga aatggtattt tgatagcagt tattttatgt ttattatctt
     2641 ctattacata ttttaaaatt gatttaatta ctactaaatc aatattttat ccactttcaa
     2701 aacaaacacc tattgaaaac agaactaatt caaaacctac atatcagatt aatgtttata
     2761 tagataatta aaaaatacaa agtttaacaa gagattaata taaataattt taataagtat
     2821 ctttcacata tgttaaattt actttaatta tattaaagcc aatgaattat tctttagaag
     2881 ctccaatgcc agtaaataca ggtaaagaaa aaattactac aattgtagat taaaaatgtt
     2941 ctaatattta aaaaaatgca aaagtgtaag aagggtaaaa atctgttata atacattaaa
     3001 aactaaattt agaaaaattt acataattag aaaagtgatt tgataaaaaa taaagttaaa
     3061 tattaactaa gtaaatttga aaaagaaaaa atataaagta caaatttaaa aggaggtaac
     3121 taatgagaaa gaagttggaa gatatgactt tagaggagtt atggaacttg tttcctatat
     3181 ttttgactac tcacaaagaa tgttggaaaa attggtttaa agaagaacaa tatagactaa
     3241 ctggaatatt gccaatgcaa aaaattgtta gaataagtca tattggaagt acttctgtga
     3301 gtacgattta tgccaagccc attgttgaca tactgattga gataattcct gattataata
     3361 tgaaagatat taaagatttg attgagtcga atggatatat ttgtatgtct gagagcagta
     3421 gtaggctttc acttaataaa gggtatacag tagaggggtt tgaagaacga gtttttcatt
     3481 tgcatataag gtatgtaggt gataatgatg agttgttttt tagagattac ttaattgacc
     3541 acccagatgt tgcaaaggag tatgaaaaac ttaagttgga cctttggaag aagtatgagc
     3601 ataatcgtga tgcttataca gatagtaaac tagattttgt tagggagttt actgaaaagg
     3661 caaaggtgtt gtattctaat agatataatt agaaataggt acaattagat atttatctta
     3721 tatattgtat tatatttttt aagaaagtaa ttaatagaaa aagggcttgt ttcaaaatgg
     3781 agagataaat ccctttttca ttgttgaaag gataaaaata tatttgattt catgtaaaaa
     3841 agagtgcctc atcaactaaa aagtttattt tgagacacac acatttttac taaggtaaat
     3901 aaaatatcac atttgattaa attttaagtt aatgaatata tgtgtaaata tttaattata
     3961 agttattata attttacata gtatcaaaca atctaatttg tattatataa gataatctaa
     4021 ttttagacta aaataatcta atataaagaa ataaagaaaa ttcactctca gtataagaaa
     4081 tatccaattt tccattgtac ttattaacaa tttctctaat attctcaata ccatatccgt
     4141 gagaattact atctttttta tctgaaataa atagttcttg ttttctattg ataggattta
     4201 ctttgttgtt tgtcaataaa tagctaaaat actccttctc acaacgtgcc tttaatacca
     4261 cttttctatt tgacaaatca ggaattttca aagatgcttc tatagcgtta tctaaagaat
     4321 ttgaaaaaat agtacataaa tctataggtt ctatacccaa gtgattgtct atatctatat
     4381 taaagtgcac atcaacacca ttttcttcca aacttgtatg acgaagattt aaaattgagt
     4441 taagagcttg gtttttgcaa aattgtttgc cactaagagt acctatacct atttttaact
     4501 gattaaaata ctttttagct ccagcaatat cttcaatttc taaatacgat tcaatcattt
     4561 gaaagtgatt attcatatca tgtcgcattt tgcgtatttg aatttgttga ttttctagta
     4621 agttatagta tttttctttt aggtgatttg cttgtttttc tatcttcatc tttacatttt
     4681 caatcaatgg ctgtaaaaga agtatcacac cgatatttgc cagaattgct gacaagatga
     4741 ttagataagt gccataaggt aagttgccta agataatatt cttattacta cccataggga
     4801 ttaaattagt aggtggcaga ataatagcaa ttaatattga taaaaatgaa gatatacata
     4861 ccatatcaat aaaaatccat attttatttg ataagtattg ttttatattg accaatctat
     4921 tctttaggaa aatatgtatt aaaaaccaaa ataataattt aattagagca gaaattatct
     4981 ggactattga aagtattaaa atatactctt ttgagtgtat aagttcctca tatatagggt
     5041 tgtttataaa aagaaaatta agcccaatta taataggata aagtatcatg acaactgata
     5101 ttataggaat taatttgctt ttgctacata ttataagtag tataaaatat ccaaatagtg
     5161 taaacaaaat atttacgggg tcggaaagcc atattatcat ttgactaata atagagtagc
     5221 ccaaaatcaa tatgattttg aaaatccaat gattcctaaa gtctataaaa taggaagtca
     5281 acttataaag aaaaaaggct atgaaaatat tgattaatat tagaataaaa tttatgcttg
//
LOCUS       DBGJFF010000195         5305 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.200, whole genome shotgun sequence.
ACCESSION   DBGJFF010000195 DBGJFF010000000
VERSION     DBGJFF010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5305
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.200"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(317..517)
                     /locus_tag="ACOIAF_003630"
     CDS             complement(317..517)
                     /locus_tag="ACOIAF_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011723062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HGZ2670610.1"
                     /translation="MNNGIVKWFNNEKGFGFISMESGDDVFAHFSAIQTSGFKSLEEG
                     QKVSFDIVKGARGPQAENITIL"
     gene            complement(742..918)
                     /locus_tag="ACOIAF_003631"
     CDS             complement(742..918)
                     /locus_tag="ACOIAF_003631"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670611.1"
                     /translation="MFRDKMDKCTHMLTAYIGSSYDYCDFIDTQLDDFILEYGEKVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(972..1061)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(1241..1441)
                     /locus_tag="ACOIAF_003632"
     CDS             complement(1241..1441)
                     /locus_tag="ACOIAF_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGZ2670612.1"
                     /translation="MPKFICNLKKYRQLKELTQEQLAEKVGVRRETIMRLEAGKYNPS
                     LKLAIDISKIVDTPIDELFIFD"
     gene            complement(1443..1793)
                     /locus_tag="ACOIAF_003633"
     CDS             complement(1443..1793)
                     /locus_tag="ACOIAF_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3796 domain-containing protein"
                     /protein_id="HGZ2670613.1"
                     /translation="MIQKKFFMGFCGFLGFLSLRYFSSGNVADLTYIGFFAFFSNFII
                     AKINGDKADERYVQDEKAAMAFTGQLAIIELFILWCITIVSRNVELMCVLLSITYAIT
                     LNVYAIKLYILEEK"
     gene            1955..2188
                     /locus_tag="ACOIAF_003634"
     CDS             1955..2188
                     /locus_tag="ACOIAF_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670614.1"
                     /translation="MTKYDFNFKLKVAKFYLNREGRDILIVKQYGVYNHSQVERWVNI
                     LGECALKIKKIFYTIWFKLDVINYMMITKYSVQ"
     gene            complement(2621..2821)
                     /locus_tag="ACOIAF_003635"
                     /pseudo
     CDS             complement(2621..2821)
                     /locus_tag="ACOIAF_003635"
                     /inference="COORDINATES: protein motif:HMM:TIGR04223.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3180..3668)
                     /locus_tag="ACOIAF_003636"
     CDS             complement(3180..3668)
                     /locus_tag="ACOIAF_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HGZ2670615.1"
                     /translation="MKNKKGFTLVELLVVIAIIGILAIVALPALFKNIEKAKIAKLEA
                     DISAIKSASLSYYADESKYTEGNIIWWTKKDGKITVNSGIGDEDPLAHKIENLGMPYN
                     GSYTLVSSNGSEEYLELNIIIDGEISKSGLDKLEEDYGSSIKIPNDKNMIITFLSNKS
                     DN"
     gene            4472..4846
                     /locus_tag="ACOIAF_003637"
     CDS             4472..4846
                     /locus_tag="ACOIAF_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HGZ2670616.1"
                     /translation="MKEEILIEKLLRAELIVMEYLWKKDTLMPKKEIVKEIKQIYGWH
                     KSTIKILLKRLVAKRYLARYIINSQSHYKIIDSKEYYTFKKKVLKSSKSIKIMRSLTT
                     THKNVSKEKLDLLDEYYRNLKE"
     gene            4951..5305
                     /locus_tag="ACOIAF_003638"
                     /pseudo
     CDS             4951..5305
                     /locus_tag="ACOIAF_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430377.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
BASE COUNT         2134 a          648 c          610 g         1913 t
ORIGIN      
        1 ttttttatta acaaattact aaaagtgtta tatcagaatt gaatacaaaa gaaagagtat
       61 gtatttatga gatagttgaa aattttggtg tgccacaact aaaattatta tattactata
      121 tattctaatt aaaagagtac ttgtaggttt aagtacatac tttataaata tatttttgtc
      181 agatttattt tataaaaagt atttagacta agtattattt ttaagtattt tcttgttgaa
      241 gttatttttt ccttttttac aaaatgcgta aataaaaaaa agactcttaa aagagtcttt
      301 ccgaaaaatc tatatattat aatatagtta tattttctgc ttgaggacct ctagcgcctt
      361 taactatatc aaagcttact ttttgacctt cttctaatga tttaaatcct gaagtttgta
      421 tagctgagaa atgagcaaat acgtcatctc cactttccat tgatataaat ccaaatcctt
      481 tttcattatt aaaccatttt actattccat tattcattaa aaatactcct attaacctat
      541 atcaagatat acttgattta cttgtgtatt accacaaata ctattgtatc atagattaac
      601 ataaatgtct atttaattat aatttttagt taacattttt atttattctt aatatgaaaa
      661 taaatttaaa cattttatat taccaatata ttaatgtaaa tgtagataaa actaaaaagg
      721 gttttctaga aaaaaatctg tctaattata cttacttacc aataccatca cttgatgcaa
      781 gcaagattcc acaacttttt ctccgtactc taatataaaa tcatctaact gtgtatctat
      841 aaaatcacaa taatcatatg aactaccaat ataagcagtt aacatatgtg tacatttatc
      901 cattttatct ctaaacataa tgtatttatc cccctaaaaa actttcatga aaaacaaaaa
      961 gaaaatatct ttttggcaac tggctgacat aacttatttt ataagcgtta gtccctatag
     1021 ctttgcgtca ctaaatttct ctagttttgc cgatttagtt ttattctaca gattaataat
     1081 acaatagaat tagttattat tcaacatatc tagctgaaaa attaataaat ttatatatga
     1141 acgttaaatt atctgaaaaa ataaaaagcc cctctgtcag gagtgactta aaaattgaat
     1201 ttaagtttgt atttaaaaca catttttaat aatgcaaaaa ctaatcaaaa ataaacaatt
     1261 catcaatagg tgtatctact atttttgata tatctattgc taatttcaaa gaaggattat
     1321 actttcctgc ttctaaacgc ataatggttt ctcttctaac tcctactttt tctgctaatt
     1381 gttcttgagt taattctttt aattgtctat atttttttag gttacaaatg aatttgggca
     1441 tattattttt cctctaaaat ataaagtttt atagcataaa catttaaagt gattgcatat
     1501 gtaattgata gtaaaacaca cattaattct acattcctag acactatagt aatacaccat
     1561 aaaatgaata attctataat agcaagctgt cctgtaaatg ccatagctgc tttttcatct
     1621 tgtacatacc tttcatctgc tttatcacca tttatttttg ctataataaa gtttgagaaa
     1681 aaagcaaaaa atccaatata tgtaaggtca gcaacattgc cagatgaaaa atatctaaga
     1741 cttaaaaatc caaggaaacc acaaaaaccc ataaaaaatt ttttctgaat cattttttat
     1801 cctccttagt aattttaaaa gagatatatt tatatcttta tgatacaaat atatctcttt
     1861 tatattcttt tgtcaataga aaatcagtat tatacaatat ttgtactgca ccataattgt
     1921 tagagcaaaa atctactgct tgagatgttt atgtatgact aaatatgatt ttaatttcaa
     1981 attaaaagta gctaaatttt atttgaatag agaaggaaga gatattttaa tagtaaaaca
     2041 atatggagtt tataaccatt ctcaagttga aagatgggtt aatatattag gagagtgtgc
     2101 actaaaaata aaaaaaattt tctatactat atggttcaag ttagatgtta taaactatat
     2161 gatgataact aaatatagtg tacaataaat cgctaagtat tttggaataa acaatagcac
     2221 agttatagca acatgaaaat aaaaattttt acatagtggt gttgatgcct tttccaaaat
     2281 gaaaggaaga ccataatgaa taaaaacaaa aacactaaag aaaaaataaa ttaacaagag
     2341 aacaagaatt agaatatgaa aataaatttt taaaagatga gcttgagttt ttaaaaaaga
     2401 tgtgtgcctc aggaaagcca cattaaaaaa cagtggtatc attattaatc aaaagtaagt
     2461 tagatgtatt atcaaaaaat tagaacttaa atgcatagta ttttttcata aatcacataa
     2521 atataactcc tataaatgta taattggaaa agtaaaaaat aataaaacaa atagaagatt
     2581 taaaacatca atagcatatc agaaaataac aaatgatatg ctagaattta taaaatatat
     2641 ctaatgataa aaatggaaaa tcaattataa aaaaggcata tctcgattca ttttttgtta
     2701 tatagtcaaa taagaatact atagatgaaa gatacgattt tgcagatgta ttaagtaagt
     2761 attctgatgt ttttggttga tacacatgca ataaagaagt tgtgtttgca gataaattag
     2821 caaaactata gtataagttg caacaataaa gaaaatgaaa attatcatac taaagcataa
     2881 ggcatgttac aattttatta gataggacat taacatacac taaagtactt tttactataa
     2941 agagtactta tttttttgaa attcattaat gtataaacta tcaagaatat tactcaacat
     3001 aatttaaaaa caacttgaat aggtattttt aaattatgtg atataataaa aaatagaagt
     3061 gtatgccaat acacttctgt agttataact aagcattcta ttcttgagga gtgctttttt
     3121 catttttata accaactctt tttactttaa caataattat cagacctata tatttatttt
     3181 taattgtctg atttattaga taaaaaagtt attatcatat ttttatcatt tggtattttt
     3241 attgaactac cataatcttc ttctaattta tctagaccac ttttacttat ttctccatct
     3301 ataattatgt ttaattctaa gtattcttca ctaccattag atgacactaa agtgtacgaa
     3361 ccattataag gcatgcctaa attttctatt ttatgtgcca aagggtcttc atcaccaata
     3421 ccagagttta ctgttatttt tccatctttt ttagtccacc atattatgtt tccttcagta
     3481 tattttgatt catctgcata gtagctaaga gacgcacttt ttattgcact tatatcagct
     3541 tcaagtttag ctatctttgc tttttctata tttttaaata atgctggaag tgctactatt
     3601 gccaatattc ctattatagc aattactact aataattcca ctagagtaaa tcctttttta
     3661 tttttcattt atttcccctc catataaaat taataatata taaattttta ttttgttgat
     3721 ttctactttc tatattttac catatataga caaaaaaatc actttctatg acaaatatac
     3781 tattatttta caatttatgg aaatgattta aaatgacatt tcttgttaga aaagatataa
     3841 gaaatggatt aaaataaaga aaactacagt catatcaatg tcaataatat tatttaaaag
     3901 cagatgaaaa taaaaagagg actcagaaaa taatgtctat ttaattatac ttacttacca
     3961 ataccataac ttgatataaa caagattata ctacattttt ttatgctcta gtataaaatc
     4021 atctaactgt gtatctataa aatcaaaata attatatgaa ctatcagtat aataagtcaa
     4081 aatatgtata catgaataca tattttaaaa gatatatatt attacatacc taaaaaactt
     4141 tcataaaaac aaaaataaaa tatgctttta gtaacttgtt aacataactt ataatttatt
     4201 ttataagcat tagcctatat agctttgtgc tactaaatct ctttgattct aacgatttag
     4261 tgctattcta caactaaaat aatacaatag agctatttat tgtttggtag cattgtttgt
     4321 aaattaaaca aatttataca tatgaatatt attttacgtt caaaaaaaca gactaaatat
     4381 gtagaatatt tggtaaaact tacctctagt ttttgtctta aaaagctcct attattaact
     4441 taagttagaa gaattaagga agtgacataa attgaaagaa gaaatattaa tagaaaaatt
     4501 attaagagca gaattaatag taatggagta tttatggaaa aaagacactc taatgcctaa
     4561 aaaagaaatt gtaaaagaaa taaaacagat ttatggatgg cataaaagta caataaaaat
     4621 actactaaaa aggctagttg ctaaaaggta tttggctaga tatattataa attctcaatc
     4681 tcattataaa ataatagata gtaaagaata ttatactttt aagaaaaagg tactaaaatc
     4741 tagtaaaagc ataaaaataa tgcgttcact taccactaca cataaaaatg taagtaaaga
     4801 gaagttagac ctattagatg agtattatag aaacttaaaa gaatgatata gtaaaaatcg
     4861 aatgtaatta tttaccttaa ttcttttgtt tgtaaaactt tattgataca gaatttaaca
     4921 tatacataaa ttggtaaagg aatgtatact atgaaaatta gtaaattacc agaagcggaa
     4981 ttaaaagtta tgagatatat ttgggaatca gaaaaggtat taatatcaag agaaatcacc
     5041 aagtctatgg aacaaaaata tgaatggaaa gatacaacta tatttacagt attaaaaaga
     5101 ttactaaaga agagagtttt tagaagcgga gaaaatagat aagtgtacac actataatgt
     5161 attagtaaaa aaaaataata tcagagattt taaacaaaag aatttttaaa gaatacacat
     5221 aaaaattcaa tattaagttt aatgtcagca ctatgtagta aggatgatga agtagatggt
     5281 gatattttaa atttattaga agaat
//
LOCUS       DBGJFF010000196         5304 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.201, whole genome shotgun sequence.
ACCESSION   DBGJFF010000196 DBGJFF010000000
VERSION     DBGJFF010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5304
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.201"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            142..1623
                     /locus_tag="ACOIAF_003639"
     CDS             142..1623
                     /locus_tag="ACOIAF_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HGZ2670617.1"
                     /translation="MKKNRLIAKNISIAFIVLFFFSVFTFFYVGNINRVLEYETNDII
                     TVTIAGWIILSFLFLGIIIYILYSKANSQKTIEKVAYTDFVTGYSNWRKFELDVTNLL
                     KKTSQNNKYAMVIFDIDKFKAINDIYGHKKGNLILKDIADTLNELTDINETFARVSAD
                     NFNILLTYNKKEDIINIIKKIMANNELVNLSFGIYEIKDKDLSVSVYSDRASLAKSSI
                     KNNSDVNFAFFNDKLREKLLFEDKIEKEMEYALESGQFVMYLQPKYNIKLDKFCGSEA
                     LVRWQYTEKEVIYPGDFIPIFEKNGFIRKIDMYILEQACKEIRSLFDKGISPLPISVN
                     FSRVDFFKKDFIENIVNICDRYKIPYSLIEIEITESSMFGDTDTLFNVSRNLQDIGFI
                     VAMDDFGSGYSSVNMLKNIPLNVIKLDRGFFVDDKDVDKSQIVIKSIVSLIKQLGIRV
                     VAEGIETRSQIEMLKKANCDIVQGYYFSKPLPIKEFEKLVYKI"
     gene            1656..5093
                     /locus_tag="ACOIAF_003640"
     CDS             1656..5093
                     /locus_tag="ACOIAF_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HGZ2670618.1"
                     /translation="MIRAKSLKNVLSIVLIFLFTIISFSDFAFKTNVQEEENSKINLR
                     EISLQSSNAIKIKLDSSLSFIKSVANMVSYFNTIDNSKIVPLLANISKESDFQRISIV
                     SPDGISHTSDGKIINVSKYQYFKKAIKGESNISEVVKSPIDGKDTFVIATPVFNKNNK
                     IIGVLYGSYYNDKLAEFIDITSFNSEGYIDIFESSGQFVLKSSHTNKLIKSSSNIYDI
                     FKKASFDDGYSYKKIYEDTKNNKSGFTSYEYKGENRYATYTPIGVNDWYVISVIPQYT
                     VRRNIDKINQLAFSLIFKVFLLFIMLITYIIYKNNKSQKDILKANKEISSITNSIPGG
                     VQKCTLGETCNINFLSDGFIELFGYTREEIKTLFNNDFYSLIHKDDVERIKKEIFKQL
                     YKNKTVELEYRIITKNGNTVWVLDKGELITEPDGKEYFCCVTVDITRTKQLEQEMEQK
                     NLELEMISENVLGGIIITEFNDDFTIKYANSGYLSMIGYTREQLENELKYKACNLVFY
                     EDRKKALESIQHQLNTKGIAVYQHRIRRRDGNIIWVSIKGRLIVDDDGIEKGIWILND
                     ITDLKNAEQKLKINEERFRIALKQTSNTIFEYDIKTKSLLNTSKELDKYNIYKITKNV
                     PYSLVELGVIHKDFSDDFINMYENVINGAKKASCVVKMKLPDENYIWNKVTLTTIFDE
                     DGNPVRAIGIQEDISKQKEAEIRYNQEGKYHSAILSEAMITYEINVSKDTFIKGNESW
                     QKPFGIKPDNNYSQMVKTIANSIVHKDNKETFLNTFLCENLLDAYKNNVTEIKLEYRR
                     MNQENKMIWVICTMHLIKDSITNDIKALAYVKDIDKQKNEEIRLKLKAERDTLTGLYN
                     KGTTEKLINDFLNSEEASLGMHAFLIVDIDNFKSVNDNLGHVFGDKVLFEVSKNLKPI
                     FRKDDIVGRIGGDEFIVFLKNIQSFENIKKKAEKICDIFRNSYTENKKTYKISGTVGI
                     SFVPEHGTVFEELYKKADIALYYAKSKGKDKYALYNDCIYQNELENNISDNNIKLADK
                     CEKSKPVLKEILDSIDNYIYIVNPNTYELLFVNNKVSRLDLEIKLGETCYKSLLKSDR
                     PCNNCPLNGLEENKHSKFKIETNTFGKNISIASWINCIDGEYNCLIECIDSSLYKNK"
     gene            5111..>5304
                     /locus_tag="ACOIAF_003641"
     CDS             5111..>5304
                     /locus_tag="ACOIAF_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HGZ2670619.1"
                     /translation="MLNSITLILGIIFTIYYFYLKIVFGGISFSEIFLVVGIVLIIYQ
                     IFKKKIKEKKVFYRVLKIII"
BASE COUNT         2226 a          502 c          821 g         1755 t
ORIGIN      
        1 tatcttttag atgataatga tatttattta aatactcttt attaacaatt tataaaactt
       61 aattgtaaaa aatacttatt aatagtaaaa taaagtgaat caattatatt ataattgcta
      121 aagagagtga ggtgtagcta tttgaaaaaa aatagattaa tagcaaagaa tatatcaata
      181 gcatttatag tactattctt ttttagtgta tttacattct tttatgtagg taatattaat
      241 agagtgttag aatatgaaac aaatgacata ataacagtta caattgctgg atggattata
      301 ctttcatttt tatttttagg tattataatt tacatattat attcaaaagc aaatagtcaa
      361 aaaactatag aaaaagtagc atatacggat tttgtaacag gttattccaa ctggagaaag
      421 tttgaattag atgttacaaa tttattaaag aaaacatcac aaaataataa atatgcaatg
      481 gtcatattcg atattgataa atttaaagca attaatgata tatatggcca taaaaaggga
      541 aatttaatat taaaagatat tgcagatact ttaaatgaat tgactgatat caatgaaact
      601 tttgctcgtg taagtgctga taatttcaat atattattaa catataacaa aaaagaggat
      661 ataataaata ttatcaaaaa gattatggca aataatgaac ttgtaaactt atcttttgga
      721 atttatgaaa tcaaagataa ggatttatct gtaagtgtat atagtgatag agcttcactt
      781 gcaaaatcat caataaaaaa taatagtgat gtaaattttg cattttttaa tgacaaatta
      841 agagagaaat tattatttga agataagata gaaaaagaaa tggaatatgc tcttgaaagt
      901 gggcaattcg taatgtattt acaaccaaaa tacaatatta aattagataa attttgtgga
      961 agtgaagccc tagtcagatg gcaatataca gaaaaagagg tcatatatcc aggtgatttt
     1021 attcctatat ttgaaaaaaa tggatttatt agaaagattg atatgtacat attagaacaa
     1081 gcttgtaaag aaatccggtc tttatttgat aaaggaattt cacctttacc tatatctgta
     1141 aacttctcaa gagttgattt ttttaaaaaa gattttattg aaaacatagt aaatatatgt
     1201 gatagatata aaataccata tagtttaata gaaattgaaa ttactgaatc tagtatgttt
     1261 ggagatactg atactctatt taatgttagt agaaacctac aggatattgg ctttatagtt
     1321 gcaatggatg attttggctc tggatattct tctgtaaata tgcttaaaaa tataccatta
     1381 aatgtaataa aattagatag aggatttttt gttgatgata aagatgttga taagagtcaa
     1441 attgttataa aaagcatagt ttctctcatt aagcaattag gaataagggt tgttgctgaa
     1501 ggaattgaaa caagaagtca aatagaaatg ttaaaaaaag caaattgtga tattgttcaa
     1561 ggatattatt tttcaaaacc attaccaatt aaagaatttg aaaaattagt ctataaaata
     1621 tagtcagcga gtcttaaatg ggaggtggtg attaagtgat aagagcaaaa tcgttaaaaa
     1681 atgtactgag tattgtttta atatttttat ttacaataat ttctttttct gattttgcat
     1741 ttaaaactaa tgtccaggaa gaagaaaata gtaaaattaa tttaagagaa atatccctac
     1801 aaagttccaa tgctataaaa ataaaattag atagttctct atcctttatt aaaagtgtgg
     1861 caaatatggt ttcttatttt aatactatag ataactcaaa aatagttcca ttattagcta
     1921 atatatcaaa agaatctgac tttcaaagaa tctctattgt atctcctgat ggaataagtc
     1981 ataccagtga tggcaaaatt atcaatgtgt caaagtatca gtattttaag aaagctatta
     2041 aaggagaaag taatatatct gaagttgtga aatctccaat tgatggaaaa gatacttttg
     2101 ttatagctac tcctgtattt aataagaata acaaaataat tggtgtttta tatggttctt
     2161 actacaatga taaattagct gaatttatag atataacaag ttttaatagt gaagggtaca
     2221 tagatatatt tgaatctagt ggtcaatttg ttttaaaatc ttcacataca aacaaactga
     2281 ttaagtcaag tagtaacata tatgatatat ttaaaaaagc ttcttttgat gatggttata
     2341 gctataaaaa aatatatgaa gatacaaaaa ataacaagag tggctttacc tcatatgaat
     2401 acaaaggaga aaatcgttat gctacttata cacccattgg tgtaaatgac tggtatgtaa
     2461 tttctgtgat accacaatat actgtaagaa gaaacattga taaaataaat caattagcat
     2521 tttctcttat tttcaaggta ttcttattgt ttattatgct tataacatat attatttata
     2581 aaaacaataa atctcagaaa gatatactaa aagctaacaa agaaatctca tctattacaa
     2641 atagcattcc tggaggagta caaaagtgta ccctgggtga aacatgcaat attaattttt
     2701 taagtgatgg atttatagag ttgtttggtt atacaagaga agaaataaaa acattgttta
     2761 ataatgattt ttatagttta atacataaag atgatgttga aagaataaaa aaagaaattt
     2821 ttaaacaact ttacaaaaat aaaactgttg aattagaata taggattata acaaaaaatg
     2881 gtaatacagt atgggtttta gataaagggg agcttataac agaacctgat ggaaaagaat
     2941 atttttgttg tgtaactgta gatattacta gaacaaaaca attagaacaa gagatggaac
     3001 aaaaaaatct agaacttgaa atgatatcag aaaatgtttt aggtggtata attataacag
     3061 aatttaatga tgattttact ataaaatacg ctaactctgg ttatctttca atgattggat
     3121 atacacgtga acaattagaa aatgaactta aatacaaagc ttgtaatctt gttttttatg
     3181 aagatagaaa gaaggctctt gaaagtatac aacatcaatt aaacacaaaa ggaatagctg
     3241 tatatcaaca tagaataaga agaagagatg gtaatattat ttgggtttct ataaaaggaa
     3301 ggcttattgt tgatgatgat ggtattgaaa aaggtatttg gattctaaat gatataacag
     3361 atttaaaaaa tgcagaacaa aaacttaaaa taaatgaaga aagatttaga attgcattaa
     3421 agcaaacatc aaacactata tttgagtatg atataaaaac aaagtcactt ttaaatacta
     3481 gtaaagagct tgataaatat aatatttata aaattactaa aaatgttcca tattctctag
     3541 tagaattagg agttattcat aaagatttta gtgatgattt tataaatatg tatgaaaatg
     3601 taataaatgg agcaaaaaaa gcaagttgtg tagttaagat gaaacttcca gatgaaaatt
     3661 atatatggaa taaagttacc ttgactacga tttttgatga agatggtaat cctgtaagag
     3721 caattggtat acaggaagat ataagcaaac aaaaggaagc agagataaga tacaatcaag
     3781 aaggtaagta tcattcagct atattgtcgg aagcaatgat aacttatgaa ataaatgtat
     3841 caaaggatac ttttatcaaa ggaaatgaaa gttggcaaaa accatttgga attaagcctg
     3901 acaataacta ttctcaaatg gtaaaaacta tagcaaatag tattgtacat aaagacaata
     3961 aagaaacatt tttaaataca tttttatgtg aaaacttatt ggatgcatat aaaaataatg
     4021 tgactgaaat aaaattagaa tatcgaagaa tgaatcaaga aaataaaatg atttgggtca
     4081 tttgtacaat gcacttaata aaagattcta ttacgaacga tataaaagca cttgcttatg
     4141 ttaaagatat tgacaagcaa aaaaatgaag aaattagatt aaaattaaaa gctgaaagag
     4201 atactcttac aggattatat aataaaggta ctacagaaaa attgattaat gatttcttga
     4261 actctgagga agcaagctta ggaatgcatg catttttaat tgttgatata gataatttta
     4321 aatctgtgaa tgataattta ggacatgtat ttggagataa agtattattt gaagtttcta
     4381 aaaacctaaa acctatattt agaaaagatg atatagtagg tagaattggt ggagatgaat
     4441 ttatagtatt cttaaaaaat attcagtctt ttgagaatat caaaaagaaa gcagaaaaaa
     4501 tatgcgatat atttagaaat tcttatacag aaaataaaaa gacatataaa atatctggaa
     4561 cagttggaat ttcatttgta ccagaacatg gaactgtatt tgaagaactt tataaaaaag
     4621 cagacatagc actttattat gcaaagagca aaggaaaaga taagtatgca ctttataatg
     4681 attgtatcta ccaaaatgaa cttgaaaata atataagtga taataatata aagttagcag
     4741 ataaatgtga gaaatccaaa ccagtactta aagaaattct tgattctatt gataattata
     4801 tctatatagt gaatccaaat acttatgaac ttctttttgt caataataag gtgtctcgtt
     4861 tggatttaga gataaaatta ggagaaacct gttataaatc actattaaaa tctgatagac
     4921 catgtaataa ttgccctctt aacggattag aagaaaataa gcattcaaaa ttcaaaatag
     4981 agacaaatac atttggtaaa aatatcagta tagcaagttg gattaattgc atagatggag
     5041 aatacaactg tcttatagag tgtatagatt cttctttata caaaaataaa taaaaaagga
     5101 ctatgaagat atgttaaata gtataacttt gatacttggt ataatattta caatttacta
     5161 tttttatctc aaaatagttt ttggaggaat ttcatttagt gagattttct tagttgtagg
     5221 aattgtacta attatctatc aaatatttaa gaaaaaaatt aaagaaaaga aggtttttta
     5281 tagggtgtta aaaataataa tcag
//
LOCUS       DBGJFF010000197         5221 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.202, whole genome shotgun sequence.
ACCESSION   DBGJFF010000197 DBGJFF010000000
VERSION     DBGJFF010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5221
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.202"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            495..1802
                     /locus_tag="ACOIAF_003642"
     CDS             495..1802
                     /locus_tag="ACOIAF_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670620.1"
                     /translation="MKEIADAITSEYLSYGINEDISIYEGRFCIYLDKKYRCNGKIYY
                     KMTPPISISFKADIGCVEEIDNEDDNLALDYDNAILEVHGYKIISITINTLSEFSVEG
                     YINDDCIKSKNSYVEYVDFNIINLDKIPGKLIKYNDKVYAGRIEFDINDYVVTIDKRY
                     DYRKELKSELKSKSGAIITHIGRIRRKDGRIFRTNNTINLLDRISTALSFMCGRYVGF
                     CLAKGYRSGNEVYRIWNENQISPFRYVPTWSDTLSNYHNMEKYISLMCKKLEDFYYGS
                     AIKSVVDWYIESLGSATMENNIISVQIALETLSYVILVEQNKILTDEAFDCNLTSKNI
                     RLLLDTCKIPYGKHELNIFDNIIKNKFDDGVDLVIYLRNSIVHPSRKTHRAVLEVEDI
                     WNIISIGTRYIELVLLFILGYRGEYSNRLVERCYGEVEVVPWN"
     misc_binding    1888..2150
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2276..3079
                     /locus_tag="ACOIAF_003643"
     CDS             2276..3079
                     /locus_tag="ACOIAF_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HGZ2670621.1"
                     /translation="MKNILKKVGIFTIMLGLLGGVVGCSKPDNEKDKDASKESKKEVV
                     VGFDNTFVPMGFLDEKGDTVGFDVDLAKETFKRLGMEVKFQPIDWSMKETELNDSKTV
                     DVLWNGYSITDERKKIVSYTEPYLQNKQIIVTLSDSKINSKADLKDKEVGTQQGSTAL
                     DAVEKDKDFMNSLKGEAPVLYDTYDKALRDLEIGRTSAVVGDEVLIRYYMGQKGEDKY
                     KVLKDDFGLEDYVVATSKENPELCEKINETLKEMKKDGTFDKIYDKWFK"
     gene            3117..3728
                     /locus_tag="ACOIAF_003644"
     CDS             3117..3728
                     /locus_tag="ACOIAF_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HGZ2670622.1"
                     /translation="MLQGLEIVIAMFCITLIVSIPLGIGVAFLRLSKNKLVSGITQCY
                     ILIMRGTPLLLQMIVIFYGLPLLGIVFDRFTAGVVAFFLNYAAYFAEIFRGGIQSIDR
                     GQYEASKVLGFDKFTMYKRVIFPQVFKRILAPISNEVITLVKDTSLVYILGLNDILRI
                     SQIAMNREASLLPLFEAGAIYLIFVAILTKGFELLEKKYSYYR"
     gene            3760..4431
                     /locus_tag="ACOIAF_003645"
     CDS             3760..4431
                     /locus_tag="ACOIAF_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HGZ2670623.1"
                     /translation="MLKIKNLNKSFKKNRVLKDISFELEEGQIGVLLGKSGAGKTTIL
                     RCINGLEEFDSGEIIIDNEVIKNKRDMAKIRGKIGMVFQNFNLFPHMTVLENIIESPV
                     NVFKVPRKEAEERARELLRLVDLEDKLNSYPFELSGGQQQRVAIARSCALMPKVLCFD
                     EPTSALDIDTIQRVVNIMNRLKDKGMTILIITHDVVFSNNVADKIISIKDGIVENVQI
                     KEKIV"
     gene            4730..5092
                     /locus_tag="ACOIAF_003646"
     CDS             4730..5092
                     /locus_tag="ACOIAF_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HGZ2670624.1"
                     /translation="MIKLYGYTKCSTVKKAKNWLKENNLEFEDIDMVQNPPSKEELKS
                     IYKTSGYDIKKFFNTSGMKYRELGLKDIVKTESDDKLLEILVSDGMLIKRPLLLDGKN
                     VLLGFKEDVWKSTLLKED"
BASE COUNT         2115 a          463 c          918 g         1725 t
ORIGIN      
        1 aaaagggaat atttacagtt taatggcagt tttaaaataa taaattaaag ctgtcatttt
       61 tatttaaaaa tttttagaaa aagtacaata gtgttaatgt gtgaattaga taaggtgtat
      121 ataatagttt agatgatatg atataaaata tgttgtaaat taaatgtttt ctttgacaat
      181 atatacacaa ggatgataat atctatatga ataaaagata gtgtgtaatt tgataatatg
      241 taattataac attatgatat aattacaata aagtatgtaa taatctgtag aaatttcaag
      301 ctagttatct aagttttcgg ataactaaat ctaaacggaa tacataatgt attaaataaa
      361 tgatgatact aaaatgatac tatttttata gaaatttatt gaggtatata attaatgaaa
      421 ataatatatt aaattatata aattaaatta tataatatat tgagttttac ataaaagcaa
      481 aggtgggaat aaaaatgaaa gaaattgctg atgctataac atcagagtat ttaagctatg
      541 gtatcaatga agatattagt atctatgaag gtagattttg catatattta gataaaaaat
      601 atagatgtaa tgggaaaatt tattataaaa tgacacctcc aatatctata agttttaaag
      661 cagacatagg ttgtgttgaa gagatagaca atgaagatga caatttagct ttagattatg
      721 ataatgctat tcttgaagtt catggatata aaattatttc tataacaata aatactttaa
      781 gtgaatttag tgtagaaggt tatatcaatg atgactgcat aaaatctaag aactcctatg
      841 ttgaatatgt agattttaat ataataaact tagataaaat acctggaaaa ctcattaaat
      901 acaatgataa ggtatacgca ggtagaattg aatttgatat aaatgattat gttgtaacta
      961 tagacaagcg atatgattat agaaaagaat taaaatcaga attaaagtct aaatctggag
     1021 caataataac tcatataggt aggattagaa gaaaagatgg tagaattttt agaacaaata
     1081 atacaataaa tcttttagat agaatatcaa cagctttgag ttttatgtgt ggaagatatg
     1141 tagggttttg tttggccaaa gggtatagaa gtggtaatga agtttataga atatggaatg
     1201 aaaatcaaat atctcctttt agatatgtac caacatggtc agatacactt tcaaattatc
     1261 ataatatgga aaaatatata agccttatgt gtaaaaagct agaagatttt tattatggtt
     1321 ctgctataaa gagtgttgtt gattggtaca tagaatctct tggaagtgct accatggaaa
     1381 acaatataat atctgtacaa attgcacttg aaactttgtc atatgtaatt ctagtggaac
     1441 aaaataagat acttacagat gaagcatttg actgtaattt gacatctaaa aatataagac
     1501 tcttacttga tacatgtaag ataccatatg gtaaacatga attaaatata tttgataata
     1561 taataaaaaa taaatttgat gatggagtgg atttagttat ttatttaaga aatagcatag
     1621 ttcatccaag tagaaagact catagagctg tgttagaggt tgaagatatt tggaatataa
     1681 tttcaatagg aacgagatat attgaacttg ttttactctt tattttaggt tatagaggag
     1741 aatattcaaa cagattagta gaaagatgtt atggtgaagt ggaagttgta ccttggaact
     1801 agatttttat atttatttta aaatatatat tgacatatat tgccttatgc tataaaatca
     1861 atatcataat aataattcag aataacatta aatgctaaga aaagaaatag taaaagtagt
     1921 taatcttaaa cagagagttt ccgattggtg agaggagcaa tagattttac tttgaacaca
     1981 tctttgagca ggactctgaa agagtagaaa tactttatta ggagatttcc ggaatccttg
     2041 cacccgttat agtgctagag tataataatt ttattgtact taaagaggtt agtgtcgtga
     2101 ggcattaatg aacagaggtg gaaccacgag agttaactcc cgtcctctag cagaagctag
     2161 aggcgggagt tttttaatat aattttctaa gcgcgcagag aattatattg caatagatac
     2221 actttttaaa aaataatcaa aatattttaa aaattaaaag acgagggaga atattatgaa
     2281 aaatatatta aaaaaagtag gaatatttac aattatgtta ggtttattag gtggagttgt
     2341 aggttgttca aaaccagata atgaaaaaga taaggatgct tcaaaggaat ctaaaaaaga
     2401 agtagttgta gggtttgata atacgtttgt accaatggga tttttagatg aaaagggaga
     2461 tacagtagga tttgacgtag acttagctaa agaaacattt aaaagacttg gaatggaagt
     2521 taagtttcag cctatagatt ggtcaatgaa ggaaactgaa ttaaatgatt caaaaactgt
     2581 agatgtatta tggaatggat attctataac agacgaaaga aagaaaatag tatcatatac
     2641 agaaccatat ttacaaaata aacagataat agttactcta agtgactcaa aaataaattc
     2701 aaaagctgat ttaaaagata aagaagttgg aacacaacaa ggttcaacag cacttgatgc
     2761 agtggaaaag gataaagatt ttatgaatag cttaaaaggt gaagcaccag ttctttatga
     2821 tacttatgat aaagcactta gagatttaga gataggtaga actagtgcag ttgttggtga
     2881 tgaagtttta attagatact atatgggaca aaagggtgaa gacaaataca aggtattaaa
     2941 agacgatttt ggattagaag attatgttgt agcaacttct aaagaaaatc cagaactttg
     3001 tgagaaaata aatgaaacat taaaagaaat gaaaaaagat ggaacttttg acaagattta
     3061 tgataagtgg tttaaataga agataagcta gctgttaaga agggggcact aaaggcatgt
     3121 tacaaggttt agagatagtt atagcaatgt tttgtataac attgatagta tcaattccat
     3181 tggggattgg agttgcattt ttaagattat caaagaataa attagtaagt ggtattacac
     3241 aatgttatat attgataatg agaggtacac ctttactttt gcaaatgata gtcatatttt
     3301 acggattacc tcttttagga attgtatttg acagatttac agctggagta gtagctttct
     3361 tcttaaatta tgcagcatat tttgcagaaa tatttagagg tggaatacag tcaatcgata
     3421 gaggacaata tgaagcttca aaagtactag gatttgataa gtttactatg tataagagag
     3481 taatctttcc acaagttttt aaaagaatac tagcacctat ttcaaatgaa gtaatcacat
     3541 tagtaaaaga tacatcttta gtatatatat taggattaaa tgatatttta agaatatctc
     3601 aaatagcaat gaacagggaa gcaagtctgt taccattatt tgaggcagga gcgatatatc
     3661 ttatatttgt agctatactt actaagggat ttgaattact tgaaaaaaaa tattcttatt
     3721 atagataaag ttacaatatt ataggaggag gttttttata tgttgaaaat caaaaactta
     3781 aataaatcat ttaaaaaaaa tagagtactt aaagatattt cttttgagtt agaagaaggt
     3841 cagattggag ttttacttgg taagtctggt gcaggaaaga ccacaatatt aaggtgtata
     3901 aatggtcttg aagaatttga tagtggagag attataatag ataatgaggt aataaaaaat
     3961 aagagagata tggcaaaaat acgaggaaaa ataggtatgg tttttcaaaa tttcaactta
     4021 tttcctcata tgacagtcct tgaaaatata attgaatccc cagttaatgt ttttaaagtt
     4081 cctagaaaag aggctgaaga gagggctaga gaacttttaa gattagttga tttagaagat
     4141 aaattaaatt cttatccatt tgaattgtca ggaggacagc aacagagagt tgctatagct
     4201 aggtcatgtg cattgatgcc aaaagtcctt tgttttgatg aacctacttc tgcacttgat
     4261 attgatacta ttcaaagagt agtcaatata atgaatagac ttaaagacaa aggtatgaca
     4321 atattaataa ttacacatga tgttgtcttc tcaaataatg ttgcagataa aattataagt
     4381 ataaaagatg gtatagtaga aaatgttcaa ataaaagaaa aaattgttta gagttttata
     4441 aatataaact agggtagaat tttataatat aaataaaggg caagtaaaat caaatatact
     4501 atgccctttt gttatctcaa aatttaatct tatattttat gtttaagtta cattataatt
     4561 tataataaaa ataaaaggac tatttaaata tataatgtga ataaatgttt aataatttga
     4621 aatatgataa ttttttatgt tgaaagctta taatgattat gatataaatt ggttatagta
     4681 tacaatttat gggtataaca ataaaaaatt agaaaggaat ttttataaaa tgattaaatt
     4741 atatggatat acaaaatgct ctactgttaa aaaagctaaa aattggctta aggaaaataa
     4801 tttagaattt gaagatatag atatggttca aaaccctcct agtaaagagg agttaaaatc
     4861 aatttataag actagtggat atgatataaa gaaatttttt aatacaagtg gtatgaaata
     4921 tagagaattg ggtcttaaag atattgtaaa aactgaatct gatgataaac ttttagaaat
     4981 attggtaagt gatggtatgc ttattaaaag accattactt ttggatggta aaaatgtatt
     5041 attgggcttt aaagaagatg tatggaaaag tactttgcta aaagaagatt aggaagagat
     5101 aggtgtgttg attaaaatta ttaattaata cacctataaa tttatgtatt aaatctaatt
     5161 tttataaata ttaattagca aattcaaatc aaaaaaattc atatcaacaa acatattact
     5221 t
//
LOCUS       DBGJFF010000198         5190 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.203, whole genome shotgun sequence.
ACCESSION   DBGJFF010000198 DBGJFF010000000
VERSION     DBGJFF010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5190
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.203"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(42..1226)
                     /locus_tag="ACOIAF_003647"
     CDS             complement(42..1226)
                     /locus_tag="ACOIAF_003647"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HGZ2670625.1"
                     /translation="MLSKRLNFITPSYTIGISSKVKEMESNGVKVINLSIGEPDFNVP
                     NNAKSYGIDSLNKDYTKYDLVPGLKILREEICKKLIEENNCNYSIDEIVVSSGAKNSI
                     TNTLLALTDEGDEVLLPKPYWVSYPEMVKLVNAVPVFIDTKKENGFKLTKDELEKSIT
                     DKTKILVINNPSNPTGSVYTKDELIEIVDVCIQNKIYILADEIYEKICYTGEFTSIAS
                     LSEEAKDITITINGFSKSAAMTGLRLGYTASNKTIAKAMSSIQGHLISHPSLTAQYIA
                     YGALKDCSIDIDNMVKTYKSRRDLIKSKLDSIDNVGYVNPNGAFYIFIDLSKVSEKFE
                     YKDSFSIEFCNQFLEEYNVAVVPGIAFGMDKYIRISYACSENTFLSGLDKLKEFVYKI
                     MA"
     gene            1599..3950
                     /locus_tag="ACOIAF_003648"
     CDS             1599..3950
                     /locus_tag="ACOIAF_003648"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside triphosphate reductase"
                     /protein_id="HGZ2670626.1"
                     /translation="MVEFVKKRDGRVIPFNEDRITRAIFLAATNVAEREGIVPDYKLS
                     EQLTQEVIKFLNHKYSESVPSVEDIQDSVVKVLIETGHAKTSEEYIIYRTERSRIRNS
                     KTRLMKAIEEITFEDAEDADIKRENANINGNTAMGTMLQYGSTVSKEFCKTHILKPEH
                     SFAHDNGDIHIHDMDFLNMGTLTCCQIDVKKLFNGGFSTGHGFLREPQDIISYGALAA
                     IAIQSNQNDQHGGQSIPFFDYGLAEGVYKTFKKFYIGNLAKALKLFKGIENNDVIKNI
                     VYNTEKETNQKVGLKRDELYLNLEKEKLIQTFDIDDELVNKMQNFAFEESYRETDKKT
                     YQSMEAFIHNLNTMHSRAGAQVPFSSVNFGTDTSEEGRMVTKNLLLSQERGLGNGETP
                     IFPILIFKVKEGINLNPEDPNYDLFKLSCRVSAKRLFPNFSFLDAPFNAKYYKKGEPD
                     TEATYMGCRTRVLSNVCGSETVSGRGNISFTTVNLPRLGIKHGIINNEKANLDGFFEE
                     LDEKINLIIEQLLERLEVQGNKKMKNFPFLMGQGVWKGSDDLGPEDTLKEVIKQGTLT
                     IGFIGLAECLIALIGKHHGESKEAQELGLKIVSHMRHKMDEATDKYKLNFSLMGTPAE
                     GLSGRFTKIDKKVYGEIKGITDKEYYTNSFHVPVYYNISAYDKIEIEAPYHELTNAGH
                     ITYVELDGDPSDNLEAFETVIKAMKDLGIGYGSINHPVDRDPICGFSGVITSNICPVC
                     GRNEDESDIKFERIRRITGYLVGTVDRFNNAKKAEVRDRVKHR"
     gene            3972..4511
                     /gene="nrdG"
                     /locus_tag="ACOIAF_003649"
     CDS             3972..4511
                     /gene="nrdG"
                     /locus_tag="ACOIAF_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HGZ2670627.1"
                     /translation="MKIRMSSTISYDSIVDGPGLRMVIWTQGCIHNCKECHNPQTHDL
                     CGGFYMDTEEIINKVKSLKLQKGITLSGGEPFLQPEPLEEIAREAKINGLDVWSYTGF
                     TFEQLLDKKNRAYFKNLNLLKQIDILVDGKFIAEKKDISLKFRGSSNQRIIDVQKSLK
                     YKKVFLVEQYMKDDLSIAE"
BASE COUNT         2033 a          587 c          841 g         1729 t
ORIGIN      
        1 accattaata cattatatct aattactatt catctaaatt attatgccat tattttatat
       61 acaaattctt ttaatttatc taatccagat aagaaagtat tttcactgca agcataggat
      121 atacgaatat atttatccat accaaatgct atgccaggaa caactgcaac attatattct
      181 tctaaaaatt gattacaaaa ttctatagaa aaactatctt tatattcaaa tttctcagat
      241 actttagaaa gatctataaa tatatagaat gcgccatttg gattcacata tcctacatta
      301 tcaatagagt ctagttttga tttaattaaa tctcttcttg atttatatgt tttaaccata
      361 ttgtctatgt caattgagca atctttcaag gctccatagg ctatgtattg tgctgttaga
      421 cttgggtgag atataagatg tccttgtata gaactcattg ctttagcaat cgttttatta
      481 gatgcagtat atcctaatct taatccagtc attgcagctg acttagaaaa accatttata
      541 gttattgtta tatctttagc ttcttcgctt aatgatgcaa ttgatgtaaa ttcaccagtg
      601 tagcatattt tttcatatat ttcatctgct aaaatatata ttttattttg tatgcataca
      661 tctactattt ctattaattc atcttttgta taaacactac cagtaggatt tgaaggattg
      721 tttattacaa ggatttttgt tttatctgtt atagattttt caagttcatc ttttgttaac
      781 ttaaatccat tttctttttt agtatcaata aaaactggaa cagcattaac taacttaacc
      841 atttcaggat aactaaccca ataaggcttt ggtaataaga cttcatctcc ttcgtctgtt
      901 aaagctagta aagtatttgt tattgagttt tttgctccac ttgatacaac tatttcgtct
      961 attgaatagt tacagttatt ttcttcaata agttttttac agatttcctc tctaagtatt
     1021 tttaatcctg gaactaaatc atatttagtg taatctttat ttaaagaatc aataccataa
     1081 gattttgcgt tattaggtac attaaaatcg ggttctccta tactaagatt tattacttta
     1141 actccattac tttccatttc tttaacttta gaactaatac ctattgtgta ggatggtgtt
     1201 ataaagttta gtctttttga taacataaaa tgcctccttt taaattatta tttatattat
     1261 acgacaaatt taaagaattt acataaggaa atttgtagat atatacatta atatatataa
     1321 tagcctacac tttttgccta aaattagtgt aaaaaaaaga gaaaaaaatt ttaactttca
     1381 aagttgttag aaacgctact gttcaaaata tccacatagt atttgcaatg atttgcgcaa
     1441 gtttgactat tatgtacaaa tttggtaatc ttaatatagc aattattaag ataatccctt
     1501 taacttagtt gaaggggatt tttttagact taacatacta catatagtgg tatggggatt
     1561 aataaaatac aatatatagt aacaggaggt ttttttaatt ggttgaattc gtaaaaaaaa
     1621 gagatggaag agtaattcca tttaatgaag atagaataac aagagctata tttttagcag
     1681 caacgaatgt tgcagaaaga gaaggaatag tacctgatta taaattatct gagcaattaa
     1741 ctcaagaagt aataaagttt ttaaatcata aatactcaga atcagttcca agtgttgaag
     1801 atatacaaga ttctgtagtt aaggttctta tagaaactgg ccatgcaaaa actagtgaag
     1861 aatatataat atacagaact gaaagaagta gaataagaaa ttcaaaaaca agattgatga
     1921 aggcaataga ggaaattacc tttgaagatg cagaagatgc tgatataaaa agagaaaatg
     1981 caaatattaa tggtaataca gcaatgggaa ctatgttaca atatggaagc actgtatcta
     2041 aagaattttg taaaacacat atattaaaac cagagcattc ttttgctcat gataatggag
     2101 atatacacat acatgatatg gattttttaa atatgggaac attaacttgt tgtcaaatag
     2161 atgttaaaaa actattcaat ggtggatttt caacaggaca tggcttttta agagagccac
     2221 aagatataat aagttatggt gcattagctg caattgctat acaaagcaat caaaacgatc
     2281 aacatggagg tcaaagtata ccatttttcg attatggatt ggctgaaggt gtatataaga
     2341 catttaagaa attctacata ggaaatttag ctaaagcatt gaagttattt aaaggaattg
     2401 aaaataatga tgtgataaag aacatagtat ataatacaga gaaagaaaca aatcaaaaag
     2461 ttggtcttaa gagagatgaa ttgtatctaa acttagaaaa agagaaatta atacagactt
     2521 ttgatataga tgatgaactt gtaaataaga tgcaaaactt tgcatttgaa gaatcgtata
     2581 gagaaactga taaaaaaaca tatcaatcta tggaagcatt tatacacaat ctaaatacaa
     2641 tgcactctag agcaggtgca caggtaccat tttcaagtgt aaacttcgga acagatactt
     2701 ctgaagaagg aagaatggta actaagaatc ttctattatc tcaagaaaga ggtttaggta
     2761 atggagagac accaattttt ccaattttaa tatttaaggt caaagaaggc ataaatttaa
     2821 atcctgaaga ccctaattat gatttattta aattatcatg cagagtatca gctaaaagac
     2881 tattccctaa ttttagcttt ttagatgcgc cttttaatgc aaaatattat aaaaaaggtg
     2941 aaccagatac agaggctaca tatatgggtt gtagaacaag agtacttagt aatgtgtgtg
     3001 gttcagaaac agttagtggt agaggaaaca tatcttttac tacagtaaat ttaccaagat
     3061 tgggtataaa acatggtatt ataaataatg aaaaggctaa tttagatggt ttctttgaag
     3121 aattagatga gaagataaac ttaattatag aacaattatt ggagcgcctt gaggtacaag
     3181 gaaataaaaa gatgaagaat tttccattct taatggggca aggtgtatgg aaaggttctg
     3241 atgatttagg accagaagat acactaaaag aagtaataaa acaaggaaca ttgacaattg
     3301 gatttatagg tttagcagag tgccttattg ctcttatagg taaacatcat ggagaaagta
     3361 aagaagctca agaattagga ttaaaaatag tgtcacatat gagacataaa atggatgaag
     3421 caacggacaa atataaatta aatttctcct taatgggtac tccagcagaa ggtctttcag
     3481 gtaggtttac taaaatagat aaaaaagttt atggagaaat taaaggtatc acagataaag
     3541 aatattatac taattcattc catgtaccag tttattacaa tataagtgca tatgataaaa
     3601 ttgagataga agctccatac catgaattga ctaatgcagg tcatataaca tatgtagagt
     3661 tagatggaga tccatctgat aatttagaag cttttgaaac tgttataaaa gctatgaaag
     3721 atttgggtat aggatatgga agtataaatc atcctgtaga tagagaccca atatgcggtt
     3781 tttcaggtgt aataacaagt aatatatgcc ctgtatgtgg aagaaatgaa gatgaaagtg
     3841 acatcaaatt cgaaagaata agaagaataa caggatactt agttggtaca gttgatagat
     3901 ttaataatgc taaaaaagca gaagtaagag atagagtaaa acatagatag ttttatttgg
     3961 gggaataagt tatgaaaata agaatgtcat ctactataag ttatgacagc atagttgatg
     4021 gaccaggatt aagaatggta atatggactc aaggatgtat tcataattgt aaagagtgcc
     4081 ataatcctca gacacatgat ttatgtggag gattttatat ggatacagaa gaaattataa
     4141 ataaagtcaa atcattgaaa ttacaaaaag gcattacgct atcaggagga gaaccatttt
     4201 tacaaccaga accattagaa gaaatagcaa gagaagctaa aataaatggg ttagatgtat
     4261 ggtcatatac tggatttacg tttgaacaac tgttagataa aaaaaataga gcgtatttta
     4321 aaaatttaaa tttactaaag caaattgata ttttagtgga tggaaaattt atagctgaaa
     4381 aaaaagatat aagcttaaag tttagagggt catcaaatca aaggataatt gatgttcaaa
     4441 aaagtttgaa atataaaaaa gtgtttttgg ttgagcaata tatgaaggat gatttatcta
     4501 tagcagaata aaatataaat aaacaggatt aaatatatgc atatatttaa tcctgtttat
     4561 tttcatataa tatttcaagc ttgtataatg taacaattaa accaaaagac tcattgtata
     4621 tttgaaatta agttttttat agtttatagg tgttttatgt ggattacgaa tgatatagta
     4681 ttgatacatt atttaataga ttcttatgcc aagaaacaat taatttatag aaattcttaa
     4741 ttgttacaat ataaatatct agataagtta taagatactg ataaataatt tataaagttt
     4801 attaatctaa tattgtgtgt gaatattaag tggtcaagta gtctaaaaaa tgaaaaagat
     4861 ttaaaaaagt gttttaacta aacacataag tgttaaattc atgtataagt ataggttaga
     4921 tacataagaa ataattatgt aatatctatc tttgtaaaaa aatattaaat tttgtaattt
     4981 gtggataaat atgtttataa tgtggataat atttaatgta ataagttaaa gcaatatgaa
     5041 tcaactgttt ataatgtgga taatattata aatgtatgaa taattgcagt atttaaaaac
     5101 aaaaaaagtt aaaaaagttg ttgactctaa aagaaaaagt tggtatagta atacttgtcc
     5161 tcaagaaaag ggcaaacaaa taaagcataa
//
LOCUS       DBGJFF010000199         5176 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.204, whole genome shotgun sequence.
ACCESSION   DBGJFF010000199 DBGJFF010000000
VERSION     DBGJFF010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5176
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.204"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            55..1827
                     /locus_tag="ACOIAF_003650"
     CDS             55..1827
                     /locus_tag="ACOIAF_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGZ2670628.1"
                     /translation="MENGKFQFFKGVRNCFNMIYLHDKRYLFISMFMAIFQGIVPSIS
                     LLVSQNIINNMQTFSKSVKEILVLLIMYLIINIIPSIFQSFYGYYRIKFNKNFEKHIE
                     TMMLEKSLTLSLKDFEDDNTYDMINRAQSQKGFNITILSENLISIIRESVSTLSLIVV
                     LSQFQLWLIIVVIVIPVLKSLYSIKLGKEQYDVSVNRTWKERKAWYIDYLLSKGYAYK
                     EIMVFRIGSKLINHYKKLKEDIISQDMNIAKKNFIATLLCTVLDSIVSAGIIGFIIIQ
                     GILKKILIGDATTYIQAVQSIKDNVESIFSLFSSVIQDLFYTDLLFKFLDYNCKEDSI
                     EHIKIFEKIGDINKIELINISYKYNHSSEYSLKNINLTLNKNDPIAILGKNGSGKTTL
                     LKILLGFYDDYEGDILINGKNLKDIEKDEYIRKVSCMFQDYIKYEATLKENVAMGDVY
                     KSNLEEKVKSKLKETKLKPSIYEEDGLETILGTWFGKKQLSMGEWQKVAISRTLMKDA
                     DMYIFDEPDSSLDSEAGNELLSLYKKTFENKIGIFITHHIQHAKKITNNIVVLENGKL
                     LESGRHDELMDKKGVYFNLFCNCK"
     gene            1849..3270
                     /gene="ccpM"
                     /locus_tag="ACOIAF_003651"
     CDS             1849..3270
                     /gene="ccpM"
                     /locus_tag="ACOIAF_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0030152 - bacteriocin biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-rich peptide radical SAM maturase CcpM"
                     /protein_id="HGZ2670629.1"
                     /translation="MNQKPFIHTFKAGKSYFIYDVNTDKILKVNAAVYNYLNKIEHNL
                     EKESDWNIEIEDEINTLINYGFLKNKRVSKTMHPETKYIKYHIENNLSSIILQVTQNC
                     NLRCDYCIYSGSYSNRVHTNKRMNIDVAKRSIDYLIKNSSQLNEVSVGFYGGEPLLEF
                     DLIKQIVEYVKVVGEGKEIYFNLTTNATLFNEEIIEFFVKNNITTMISLDGGKEIHDK
                     SRKFANTGEGSHDVVLKNVRYIKNKYPEYYKKNITFNTVINSENDYISIDDFISKNET
                     LKDSNFLASTLNNNYSDKEIVSSDEYILGKSYDRFLTLLNKLKFIKDESMSPLSRIER
                     ALLDDFRYEKNIEGRLELPDVWHHGGPCIPGAFRIFVTTDGEFFPCERVSESSKMGMI
                     GNLDSGVDIEKAEKLLNIGKLYEEQCSDCWAYSYCDICIAKIDDTKCNENEIKPHDCD
                     RIRHSTENNMKDYCVLKSLGYQY"
     gene            3299..4369
                     /locus_tag="ACOIAF_003652"
     CDS             3299..4369
                     /locus_tag="ACOIAF_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04066 family peptide maturation system
                     protein"
                     /protein_id="HGZ2670630.1"
                     /translation="MEKVLIYPFNYNFFTILNHCNKISDIEINSLVSPSGWGLCNKEY
                     NVNNIITKVTNDFQSELKKNSLVWFVDSKENLDLEKFIIPKLKVALKEGKKILYTRDD
                     LNKISRYISHYEFKKINEWNLEKYEIRDIEGISQINTPIIFICGLYGGLDTFDVNIRI
                     GEELRKRGFSILQFGPKKESYLFKMIPIPEFMFDDSISSKTKIINFNHFIKDKEVEAK
                     PDLIIIEVPGELFPFSRKMVGNFGIRAFEISNAVINDCGILCIPSSIYDREYCEELKY
                     FIKGRHGINIDYFSIEKKVIDTYETEVKQKFSYMSLDENFFVEDIEDYEKTFFIHDNK
                     QLLELVDNIVNQLKEYAKVISI"
     gene            4385..4636
                     /locus_tag="ACOIAF_003653"
     CDS             4385..4636
                     /locus_tag="ACOIAF_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030152 - bacteriocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide maturation system acyl carrier-related
                     protein"
                     /protein_id="HGZ2670631.1"
                     /translation="MNYTEKELLLENIFFDVTGINFNKNNNLKKELFFSPSLHIQPRE
                     LLLVFCEVIENFNIKIPEEKLLNRQFTSFDNVLKIINMV"
     gene            4687..4890
                     /locus_tag="ACOIAF_003654"
     CDS             4687..4890
                     /locus_tag="ACOIAF_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670632.1"
                     /translation="MSFGKRIEMNNIGVETRGVCTIMCPYTCKMMASSSCNCKNNDYN
                     ARGGSYNTYYNKLIHGQRTPDGM"
BASE COUNT         2108 a          455 c          830 g         1783 t
ORIGIN      
        1 tatattaatt aataaaatat tttatttcaa ttttattaag tatggaggtg tattgtggaa
       61 aatggtaaat ttcaattttt taaaggtgta agaaattgtt ttaatatgat ttatctacat
      121 gataaaagat atttatttat ctctatgttt atggcaattt ttcaggggat tgttccatct
      181 atttctttgt tagtaagtca aaatatcatt aataatatgc aaacattttc gaaaagtgta
      241 aaagaaatat tagttttgtt aattatgtat ttaatcataa atataattcc ttcaattttt
      301 caaagttttt atggatatta tagaataaag tttaataaaa attttgaaaa acatattgaa
      361 acaatgatgt tagagaaatc tttaactttg agtttaaaag attttgagga tgataatact
      421 tatgacatga taaatagagc tcaatctcaa aaaggattta atattactat actatctgaa
      481 aaccttattt caataataag agaatcagta tctacacttt ctcttattgt tgtattatca
      541 caatttcaac tttggctaat aatagttgtt attgttattc ctgtattgaa atcattatat
      601 tccattaaat taggaaagga acaatatgat gttagtgtaa acagaacttg gaaagaaaga
      661 aaggcctggt acatagatta tttgttgtca aaaggttatg cttacaaaga gattatggta
      721 tttagaattg gttctaaact tattaatcat tataaaaaat taaaagagga tattatatct
      781 caagatatga atatagctaa aaagaatttt attgctacgc tattatgtac agttttagat
      841 tcaattgtat ctgcaggaat tataggattt ataataattc aaggtatttt aaagaagatt
      901 ttaataggag atgcgactac atatatacaa gcagttcaat ctataaagga taatgttgaa
      961 tcaatatttt cgttatttag ttcagtaata caagatcttt tttatacaga tttactgttt
     1021 aaatttctag attataactg taaagaagac tcaattgaac atataaaaat atttgaaaaa
     1081 ataggggata taaataaaat agagttaata aatatttcat ataaatacaa tcattcatca
     1141 gagtattctt taaaaaatat taatttaact ttaaataaaa atgatcccat tgctatacta
     1201 ggaaaaaatg gttcaggtaa aacgacactt ttaaaaatat tattaggttt ttatgatgat
     1261 tatgaaggtg acatcttgat aaatggaaaa aatttaaagg atattgaaaa agatgaatac
     1321 attagaaaag taagttgtat gtttcaagat tatattaaat atgaggctac attaaaagaa
     1381 aatgtcgcca tgggagatgt atataagagt aacttggaag aaaaagttaa aagtaaactt
     1441 aaagaaacta aattgaaacc aagtatttat gaggaagatg gattagaaac tattttagga
     1501 acctggtttg gtaagaagca actttctatg ggtgaatggc aaaaagtagc aatatctaga
     1561 actcttatga aagatgctga tatgtacatt tttgatgaac cagattcatc tttagattct
     1621 gaagcgggaa atgaactcct ttctttatat aaaaaaacat ttgaaaataa gattggaata
     1681 tttataacgc accatatcca acatgcaaaa aagataacta acaatattgt agttcttgaa
     1741 aatggaaagt tgttagaaag tggaagacat gatgaactta tggacaaaaa gggtgtttat
     1801 tttaatttat tttgtaactg caaataaata ttaaagggag gttaattaat gaatcaaaaa
     1861 ccatttatac acacatttaa agctggaaaa tcatatttta tttatgatgt taatacggat
     1921 aagattttaa aggtaaatgc tgctgtttat aattatctaa ataaaattga gcataattta
     1981 gaaaaagaaa gtgactggaa catagaaata gaagacgaaa ttaatacctt gattaattat
     2041 ggatttctaa aaaataaaag ggtaagtaaa acaatgcacc ctgaaactaa gtatataaaa
     2101 tatcatatag aaaataatct atcctctatt attttacaag taacccaaaa ttgtaattta
     2161 cgttgtgact attgcattta ttctggttca tacagcaaca gagttcatac aaataaaaga
     2221 atgaatatag atgtagcaaa aagaagtatt gactatttga ttaagaattc tagtcaatta
     2281 aatgaagtta gtgttggctt ttatggagga gaacccttac ttgagtttga tttgattaag
     2341 cagatagtag aatatgtaaa agtagttgga gaaggaaagg aaatatactt taatctaact
     2401 acaaatgcaa ctttatttaa tgaggaaata atagaattct ttgtaaaaaa taatataaca
     2461 actatgatta gtttagatgg aggaaaagaa attcatgata aaagtagaaa atttgcaaat
     2521 acaggagaag gatctcatga tgttgtatta aaaaatgtac gttacataaa aaataaatat
     2581 ccagaatatt ataaaaagaa tattacattt aatacagtga taaattcaga aaatgattac
     2641 atttctatag atgattttat atctaaaaat gagactttaa aagattcaaa ctttttagct
     2701 tccactctta ataataacta ttctgataaa gaaatagttt catcagatga atatatatta
     2761 ggtaagagtt atgatagatt cttgacttta ctcaacaaat taaaatttat aaaagatgag
     2821 agtatgtcac ctttatcaag aatagaaaga gccttattag atgattttag atatgagaaa
     2881 aatattgaag gtagattaga gttaccagat gtgtggcatc atggtggacc atgtatacca
     2941 ggagctttta gaatatttgt aactacagat ggagaatttt ttccatgtga gagagtaagt
     3001 gaaagttcta aaatgggtat gattggaaat ttagatagtg gagtagatat agaaaaggct
     3061 gaaaaacttt taaatatagg aaaattatat gaagagcaat gttctgattg ttgggcctat
     3121 agttattgtg atatatgtat agctaagatt gatgatacta aatgtaatga aaatgaaata
     3181 aaaccacatg attgtgacag aattagacat tctacagaaa ataatatgaa agattactgc
     3241 gttttgaaat ctctaggata tcaatattaa attttctaat gaaaggaaag atgggattat
     3301 ggaaaaagta cttatatatc catttaatta taattttttt actattttaa atcattgcaa
     3361 taaaatttca gatatagaaa ttaattcact ggttagtcct tctggctggg ggttatgcaa
     3421 taaggagtat aatgttaata atattattac taaagttaca aatgattttc aaagtgaact
     3481 taaaaagaat tctttggtat ggtttgttga ttcaaaagaa aatctagact tagaaaaatt
     3541 tataattcct aaattaaaag tagctctaaa agaaggtaaa aagatattat atacacgtga
     3601 tgatcttaat aaaatatcta ggtatatatc tcattatgaa ttcaaaaaga ttaatgaatg
     3661 gaatttagaa aaatatgaaa taagagatat agagggtata tcacagataa acacaccaat
     3721 aatatttatt tgtggtcttt atggagggtt agatactttt gatgtaaata taaggatagg
     3781 agaagaacta agaaaaaggg ggttttcaat attacaattt ggccctaaaa aagagagcta
     3841 tttatttaaa atgattccta taccagagtt tatgtttgat gattcgataa gtagtaagac
     3901 aaagattatt aattttaacc attttataaa agataaagag gtagaggcaa agccagattt
     3961 aataattata gaagttccag gagaattatt ccctttctct agaaaaatgg ttggtaattt
     4021 tggaattaga gcttttgaaa tttctaatgc agtaataaat gattgtggta tattatgtat
     4081 tccatcaagt atatatgata gagaatattg tgaagaactt aaatatttta ttaaaggaag
     4141 acatggtata aatatagact attttagtat agagaaaaaa gttatagata catatgagac
     4201 agaggtgaag caaaaatttt catatatgtc attagatgaa aacttttttg ttgaagatat
     4261 agaagattat gaaaaaacat tttttataca tgataataaa caattactag aattagtgga
     4321 caatatagtg aatcaattaa aagagtatgc taaagtaata tctatttaga attggaggtt
     4381 aattatgaat tacactgaaa aggaattact actagagaat atattttttg atgtgactgg
     4441 aattaatttt aataaaaata acaatttaaa aaaagaatta tttttttcac cgtctttaca
     4501 tatacagcca agagaattac ttttagtatt ttgcgaggta atagaaaact ttaatataaa
     4561 aattccagag gaaaaacttt tgaataggca atttacgagt tttgataatg ttctaaaaat
     4621 aatcaatatg gtataggtta ataaaggtat aaagtaagtg atcttataga aaaggggagg
     4681 atatgaatga gttttggaaa aagaatagaa atgaataata taggtgttga gacaagaggt
     4741 gtttgtacta ttatgtgtcc ttatacttgt aagatgatgg catcttcatc ttgtaactgc
     4801 aagaataatg attataatgc aagaggagga tcatataata catattataa taaattgata
     4861 catggccaaa gaactcctga tggaatgtaa atatttcagt aataagtata ctattaaatt
     4921 gatttagcat atttgattaa atttatcttt cttactttat ttccttttat tataaaaata
     4981 tgcagttgat tataattaag agtgtgattt tataataact gcataaatag tatctgttat
     5041 ataaatcacc tcttcaatta ttatagtggt aaatatagtg attaggggtg atttttacaa
     5101 tttaaaagta taaattaatt aaaagttatt ataataaatt atacttattt aaatagtaag
     5161 aattaaaagt aaagtt
//
LOCUS       DBGJFF010000200         5093 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.205, whole genome shotgun sequence.
ACCESSION   DBGJFF010000200 DBGJFF010000000
VERSION     DBGJFF010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5093
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.205"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(<1..1109)
                     /locus_tag="ACOIAF_003655"
     CDS             complement(<1..1109)
                     /locus_tag="ACOIAF_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGZ2670633.1"
                     /translation="MKSYTKAKWSVWGIITFSFVLVLFLRMSTAVVSDNLANELGFNS
                     IQISNIASFCLYAYAFMQIPAGILIDKYGARKISSLGIIMASLGSILFGLIQSIELAY
                     ISRVIVGAGTSVILLCILKIQGRWFNKSEFASATAKFSFVGNLGGVLATFPLVFLSEL
                     VGWRNSFLLIGIIGVVIGCFMYIIVRDTPKEYGFNVDTEPYEKSEKVNIVDGIKSVIK
                     NKSTWYNSMIMFSFVGLTSAFISLWGVRYIMDVYGVSKSFSAFIVSFFTYGFIFGSII
                     MDFVFAKIRSSKFNIIKFGAMIDLFIWIVIVVVYQVKPPIIVLPISFFIMGCIVMSHL
                     QVFNDTKYKNKEIYSGLATSVINTFEFIGSGIINL"
     gene            complement(1331..2095)
                     /locus_tag="ACOIAF_003656"
     CDS             complement(1331..2095)
                     /locus_tag="ACOIAF_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2670634.1"
                     /translation="MLGKLLKYELKASGRIFIPLYIAILIVAVFNGIFMNTNILQVQG
                     IGILVLTSLFMALGVLTIVVTIQRFRKNLLGDEGYLMFTLPVSTSSLILSKCITALIY
                     AVLSFIVAVFTFGVLMLFGTSGILLPEILDLFNTSFKWISENFLDILLLVVVMFISYS
                     SFILLLYTSISMGQLPKFNKHRNIVAFASFIAINIVISIVGDAVGSILPNEDTNMVYH
                     LYQSPSFMLAILGSLVVAIALFFATKFILDKKLNLE"
     gene            complement(2089..2814)
                     /locus_tag="ACOIAF_003657"
     CDS             complement(2089..2814)
                     /locus_tag="ACOIAF_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2670635.1"
                     /translation="MDKNISNVSQDGIVEFKGVNKSYGTKNVLKNIDLNIPKGKIVGL
                     LGPNGSGKSTMIKLMNGLLQPDNGEIMINGMKPSIDTKKIVSYLPERTYLNDWMKVSD
                     LLKFFYDFYSDFDVRRANEMIKSLDIDVNEKLKTMSKGTKEKVQLILVMSRNASIYIL
                     DEPIGGVDPAARSYILKTILKNYSEDSTLLIATHLISEIENICDEIIFISKGEIVLQG
                     DVEAIREEKGKSIDALFREEFKC"
     gene            complement(2816..3184)
                     /locus_tag="ACOIAF_003658"
     CDS             complement(2816..3184)
                     /locus_tag="ACOIAF_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGZ2670636.1"
                     /translation="MEWELDNNKPIYIQLVEHLKLKIISGEIKIGSKLETVRALAEDA
                     EVNPNTMQKALTELERQGLVYSQRTKGRFVTDDKEKIKAMKEEIANVEINTLKVTLEK
                     LGYDRDEMLKLITENLKGEL"
     gene            complement(3360..4901)
                     /locus_tag="ACOIAF_003659"
     CDS             complement(3360..4901)
                     /locus_tag="ACOIAF_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HGZ2670637.1"
                     /translation="MRNLQKKLMFFLFICIILQITYTTPVYSLCKLYKNNSINNFESI
                     VSNNQLISKHAIQIILIMAIICVILIFYIIYDKLNFKIKLQRIAYTDNLTGANTIDKF
                     VIDANKILCKNTQVKYALLYIDIDKFKYINDLFGYEVGNEILRNLTKIIKSNIFEEEM
                     FARISADNFIIIMKYIEEDITKRLKTIFEELDLFNNNQEEKYKLVLSCGIYFILPEDR
                     DINSIIDRANIPHKMAKGGHKSSYAFYDNKIHDQEIKEKEMENTMFSSLENKEFIIYL
                     QPKIELNTGEIQGSEALVRWKRPDKGLIPPNEFIPFFERNGFVINLDLYVLEEVCIYL
                     RKWIDAGINPVTVSVNVSRIHLYCNNFIETYKNIIDKYNIPAKYIELELTESIIFDNF
                     DILIDIMNNLKKIGFLISMDDFGSGYSSLNMLKEIPMDILKLDQKFIMETYNSKRSKI
                     IVTKVIEMAKELGMKVISEGVETEEQFKLLKEVKCDMAQGYLFGKPMPIEEFEHLIVS
                     NLIRG"
BASE COUNT         1786 a          857 c          481 g         1969 t
ORIGIN      
        1 atcaaattaa taattccact tcctataaat tcaaaagtat taataacact agttgccagt
       61 ccagaataaa tttctttatt tttatacttg gtatcattaa aaacctgtag atgtgacata
      121 actatacatc ccataataaa gaatgatatt ggtaaaacta taattggtgg ttttacctgg
      181 tatacaacta caattacaat ccatataaac aaatctatca ttgctccaaa ttttattata
      241 ttaaatttgc tagaccttat tttagcaaat acaaaatcca taattattga accaaatata
      301 aatccatatg taaaaaatga tactataaac gcagaaaaac tcttactcac accataaacg
      361 tccattatgt atcttactcc ccacaagctt ataaatgcac ttgtaagacc aacaaaagag
      421 aacattatca ttgaattata ccatgttgat ttgttcttta taacagactt aataccatca
      481 acaatattaa ccttctctga cttttcatag ggttcagtat ctacattgaa tccatactct
      541 tttggtgtat ctctaacaat aatatacata aaacatccaa ttacaacacc aattataccg
      601 attaataaaa atgaatttct ccaaccaaca agttcagaca aaaacacaag tggaaaagta
      661 gcaagaactc cacctaaatt tcctacaaat gaaaatttag ctgttgcaga agcaaattct
      721 gacttattaa accatctccc ttgaattttt aaaatacaaa gtaatattac tgatgttcct
      781 gcccctacaa ttacacgaga aatatatgct aattctatac tttgaatcaa accaaataaa
      841 attgacccta aacttgccat tattattcca agactgctaa tttttcttgc tccatattta
      901 tcaatcaaaa ttcctgcagg tatttgcata aatgcatatg catataggca aaaggatgct
      961 atatttgaaa tttgtataga attaaaacct aactcatttg ctaaattgtc tgaaacaaca
     1021 gcagttgaca tccttaaaaa taatactaaa acaaatgaaa atgtaattat accccaaaca
     1081 ctccacttag cctttgtata acttttcata tcttctccct caaatccata tatttttaaa
     1141 ttttcttgat ataacttata taaagtgtat agttattctt acaaattttt acacgcaata
     1201 tcattataca acatatgtat atatttaact agaaataaaa cactattcat attatataca
     1261 tgaattaact acataaataa taagagccat cttttaacat tactcaaaag atagctccat
     1321 ataaaaacat ttattctaaa tttaattttt tatctaatat aaattttgta gcgaaaaata
     1381 acgctattgc aactacaaga ctaccaagta ttgcaagcat aaaactagga ctctgataaa
     1441 gatgatatac catatttgta tcttcatttg gtaagatgct tccaactgca tctccaacaa
     1501 ttgatattac tatatttata gctataaaag aagcaaatgc cactatgttt ctatgtttat
     1561 tgaattttgg caattgtccc attgagattg atgtatataa taacaaaata aatgatgaat
     1621 atgaaataaa cattactact actaataata aaatatctaa aaagttttca gaaatccatt
     1681 taaaactagt gttaaacaaa tctaaaattt caggtagtaa aattccagaa gtaccaaaaa
     1741 gcataagaac tccaaaagtg aatactgcaa ctataaaact aagtacagcg tatataagtg
     1801 ctgttataca tttagataat ataagtgaac tagtgctaac aggaagagta aacattaaat
     1861 aaccttcatc ccccaaaaga ttttttctaa acctttgaat tgtaactaca atagttaaaa
     1921 ctcctaatgc cataaaaaga cttgttaata ctagtattcc tattccctgt acctgaagta
     1981 tatttgtatt cataaatatt ccattaaata cagctactat caatatggct atatataacg
     2041 gtataaatat tcttccactt gcttttaact catattttaa taatttacct aacatttaaa
     2101 ttcctcccta aataatgcat ctatagattt tcctttttct tctcttattg cttcaacgtc
     2161 tccttgtaac actatttctc cttttgatat gaatattatc tcatcacata tgttttctat
     2221 ttcacttatt aagtgggtag caatcagtaa agtactatct tcagaataat tttttaatat
     2281 agtcttaagt atatatgacc ttgcagctgg gtcaacacca cctataggtt catccaatat
     2341 atatatgctc gcatttcttg acattactaa aataagttga actttttctt tagttccttt
     2401 agacattgtc tttagctttt catttacatc aatatctaag cttttaatca tctcatttgc
     2461 tcttctaaca tcaaaatcac tataaaaatc atagaaaaac ttaagtaaat ctgatacctt
     2521 catccaatca tttaaataag ttctttctgg taaatatgaa actatttttt tagtatctat
     2581 ggatggtttc attccattta tcataatttc accattatca ggctgtaata atccattcat
     2641 tagcttaatc attgtacttt tgccacttcc attaggtcct aacagcccga ctatttttcc
     2701 ctttggtata tttaaatcaa tattttttaa aacattctta gttccataac tcttgtttac
     2761 acctttaaat tcaactattc catcttgaga aacattgctt atatttttat ccattttata
     2821 attctccttt cagattttct gtaataagtt ttagcatttc atctctatca tatccaagct
     2881 tttctaaggt tacttttaat gtatttatct ccacattagc tatttcctct ttcatagctt
     2941 ttattttttc cttatcatct gttacaaatc ttcctttagt tctctgacta taaactaaac
     3001 cttgtctttc aagttctgtt agagcctttt gcattgtatt tggattaact tccgcatctt
     3061 ctgctagtgc tctcactgtt tctaatttag atcctatttt aatttcccct gaaattattt
     3121 ttaactttaa atgttctact aattgtatat atataggttt gttattatca agttcccatt
     3181 ccatgttcat gcctccttat tatattattg tattaagttg ttaatacaat aatataataa
     3241 tacaaagtat atgtcaatta ctttttagta aaagtataaa aaattttaca aaaaaaatga
     3301 acactacatt agagtccatt tttaacacaa tttattaaat tctttatatt acagattaat
     3361 taccctctta tcaagttgga tacaattaaa tgttcaaatt cttcaattgg cattggcttt
     3421 ccaaacaaat atccttgtgc catatcacac tttacttctt ttaaaagttt aaactgttct
     3481 tctgtctcaa ctccttctga tatgactttc atacccaact cttttgccat ttctattact
     3541 ttagtaacaa ttattttgct tcttttacta ttataagttt ccataataaa tttttggtca
     3601 agttttaaga tatccatagg aatttctttt aacatattta atgatgagta ccctgaacca
     3661 aaatcatcca tagatattaa aaatcctatt ttttttaaat tattcattat atcaattaaa
     3721 atatcaaaat tatcaaatat gatactctcc gttaattcaa gttctatata ctttgcaggt
     3781 atattgtatt tatcaattat atttttgtat gtttctataa aattattgca atataaatgt
     3841 atccttgaaa cattaactga aacagtaact ggatttattc ctgcatcaat ccattttcta
     3901 aggtatatac atacttcttc aagcacatat aaatctaaat ttataacaaa accgtttctt
     3961 tcaaaaaatg ggataaattc atttggaggt attagccctt tatcaggacg tttccaccta
     4021 acaagtgctt ctgaaccttg tatctctcct gtattaagtt ctatcttagg ttgtaagtat
     4081 ataataaatt ctttattttc tagacttgaa aacattgtat tttccatctc tttttctttt
     4141 atctcttggt catgaatctt attatcataa aatgcatatg aacttttatg tcctcccttt
     4201 gccattttat gaggtatatt agccctatct atgattgagt tgatatctct atcttctgga
     4261 agtataaaat agattccaca acttaaaact aacttatatt tttcttcttg attattatta
     4321 aacaagtcta attcttcaaa tatagttttt aacctctttg ttatatcttc ttctatatat
     4381 ttcataatta taataaaatt atccgcagat attcttgcaa acatttcctc ttcaaatata
     4441 ttactcttaa taatctttgt taaattacgc aaaatttcat tcccaacttc atatccaaat
     4501 aaatcattta tatatttaaa tttatcaata tctatatata ataaagcata ctttacttga
     4561 gtatttttac atagtatttt gttagcatca attacaaatt tatctatagt atttgctcct
     4621 gtaagattat ctgtatatgc tatcctttgc aatttaattt taaaatttaa tttatcatat
     4681 attatataga atataagtat tacacatatt atagccataa taagtattat ttgtattgca
     4741 tgtttgctaa taagctgatt attagacaca atagactcaa agttatttat ggagttgttt
     4801 ttatagagtt tgcataaaga atagactggt gttgtgtatg taatttgtaa aattatacaa
     4861 ataaataaaa aaaacattaa ttttttttgt aaattcctca taaaaatttc ctcccattaa
     4921 aacacctcta ttttattagc aaataagtac atctcaaaag tttccatttc ttgtaaaatg
     4981 gatagtttat atctacttta attttgtaaa attttctgct actataatta tagaatgtag
     5041 catattcatt gtcttaacta ttgatttata tatttcatat aggtataata gaa
//
LOCUS       DBGJFF010000201         4933 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.206, whole genome shotgun sequence.
ACCESSION   DBGJFF010000201 DBGJFF010000000
VERSION     DBGJFF010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4933
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.206"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     misc_binding    437..674
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            738..2657
                     /gene="thrS"
                     /locus_tag="ACOIAF_003660"
     CDS             738..2657
                     /gene="thrS"
                     /locus_tag="ACOIAF_003660"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HGZ2670638.1"
                     /translation="MIKVALKDGSIKEFENAISVMDVAKSISEGLARNVVAASVNGEV
                     VGLDHIIDTDCDLNLFKFEDKEGKEVFRHTSAHILAQAIKRLYPEAKLAIGPSIENGF
                     YYDIDLDHRLVPEDLEKIEAEMKKIAKEDLKIERFELPRNEALELMKEQGEDYKVELI
                     SDLPESEIISFYKQGDFTDLCRGPHLPSTKKVKAVKLQSVAGAYWRGDENNKMLQRIY
                     GTSFEKNKDLEEYLHLLEEAKKRDHRKLGKELGLFMIPEEGPGFPMFLPKGMELKNEL
                     LKFWREIHRKAGYIEIESPIILNRKLWETSGHWYHYKENMYTVKIDDEDYAIKPMNCP
                     GGLIYYNSQLHSYRDFPMRVAELGRVHRHELSGALQGLMRVRAFTQDDSHIFMLPEQI
                     KDEIKGVANLIDGIYKTFGFEYNLELSTRPENSMGSDEEWEAAENGLREALEELGLPY
                     TINEGDGAFYGPKIDFHLKDCLGRTWQCGTIQLDMQLPRQFDNTYIGQDGEKHRPVMI
                     HRVAFGSIERFIGILIEHYAGKFPVWLSPTQVKILPISDKFMDYANEVKKELFDKGIR
                     VELDDRAEKIGFKIREAQLEKVPYMLIVGEKEVADNNVSVRSRDKGEIGSIKLDEFIA
                     SISKEIESRESIIQD"
     gene            3425..4516
                     /locus_tag="ACOIAF_003661"
     CDS             3425..4516
                     /locus_tag="ACOIAF_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HGZ2670639.1"
                     /translation="MIEKVEEKSKIIKTSKEYKRTIIEEKDKEWTKKLNDILSRSSFS
                     NDELDKIKALIPKEILTNENVGEVVTEDGYAKPEYDEVFKSLFTLNNEYDLLVNFIND
                     ILKDAPYANRNIKPFTQIKRIIRVETDPTINYIGEKRPRLDILAEDEENNHINIEMQR
                     AFENDYLERAEYYLSRVHGRKLEEGKEYKEIGKTIGIHILNHVKYNHIEDYVNCLRLT
                     MDGHPDIYSSKTALYFIELPKIHKSDYIVNRIMLWGKLISNPSNLDVRVIAKNDSIIK
                     KALDRLKELGSNEEYLLNLKRGAYIMNKSRNFKEEIERETAIRVAKEKDYKIIIMLKK
                     NGFKLDDILNKFNDFDLSEDEIKEVYENN"
BASE COUNT         1944 a          485 c          867 g         1637 t
ORIGIN      
        1 ttagatatta attgtattta cctatttgag tgaattactt attaatagca tttagatatt
       61 aattgtattt atttatttga ttgtgttact tattaatagt atttaagtat ttactgtact
      121 acttattaac tatattaagt attgattgta tttaagtata caaaacctaa aaagagtaca
      181 aaaattagaa gttagtttta ataaaaatta gttttagtat ttatctagtc atatctatca
      241 tgcatgtgga tacgttttaa aaattttaaa tttatgaaaa tattataata tcactataaa
      301 tcaagagatt aatttaatat aaaaatataa gaatattggt ttacagctga ttgagatgaa
      361 aaataatggg taattaatat attacattaa tgttgattga tgtataacta tctgttataa
      421 tgaaaatata ttaaaaatca tagtgttgaa gaggaagagt agagtagaaa ccattctcag
      481 agaatttggg tctatggctg agagcccatg taataggtct atttgaaaac taccttggag
      541 ctgagcttat tagcagtatt tgttttgtac tatacgtgaa agtgctcggg tgacgacctt
      601 atagtcatca agagggttgt atatacttgt atacgccaac ttgggtggaa ccgcgaatta
      661 atatcgtccc aaacatattt ggggcgattt tttattacaa aaaatctaaa aattatttca
      721 ataaggagga aaaaacaatg attaaagttg ctttaaaaga tggctcaata aaagaatttg
      781 aaaatgctat ttctgttatg gatgtagcta aatccattag tgaaggatta gctagaaatg
      841 tggtagcagc gtctgtgaac ggagaggttg taggtctgga ccacattata gatactgatt
      901 gtgacttgaa tttatttaag tttgaagata aagaaggtaa ggaagttttt agacatacat
      961 cagcgcatat attagctcaa gctataaaaa gactttaccc agaagcaaag cttgctatag
     1021 gaccaagtat agaaaatgga ttctactatg atatagattt agaccataga ttagttccag
     1081 aggacctaga gaaaatagaa gctgaaatga aaaaaatagc taaagaagat ttaaaaatag
     1141 aaagatttga acttcctaga aatgaagctt tagagcttat gaaagaacaa ggagaagact
     1201 ataaggtaga acttatatct gatttacctg aaagtgaaat aatatctttc tacaagcaag
     1261 gtgattttac tgatttatgt agaggaccac atttaccatc tactaaaaag gtaaaagctg
     1321 ttaaattaca aagtgtagca ggtgcatatt ggcgtggtga cgaaaataat aagatgcttc
     1381 aaagaatata tggaacttct tttgagaaaa acaaagactt agaagagtac ttacatttac
     1441 tagaagaagc taaaaagaga gaccacagaa aattaggtaa agaattagga ttatttatga
     1501 tacctgaaga aggtccagga ttccctatgt ttttaccaaa aggtatggaa cttaaaaatg
     1561 aattattaaa attctggaga gaaatacaca gaaaagctgg atatattgag atagaatctc
     1621 caatcatatt aaatagaaaa ttatgggaga cttcaggaca ctggtatcat tacaaagaaa
     1681 atatgtatac agttaaaata gatgatgagg attatgctat aaaacctatg aactgtcctg
     1741 gtggattaat ttactataac tctcaactac attcatatag agatttccca atgagagttg
     1801 cagagcttgg tagagttcat agacatgagt tatctggtgc attacaagga ttaatgagag
     1861 ttagagcatt tactcaagac gattcacata tattcatgtt accagagcaa attaaggacg
     1921 aaataaaagg agtagctaac ttaatagatg gtatctacaa aacttttgga tttgaatata
     1981 atttagaatt atctacaaga ccagagaact ctatgggaag cgacgaagag tgggaagcag
     2041 cagaaaatgg attaagagaa gctttagaag agttaggtct accatatact attaatgagg
     2101 gtgatggagc attctatggt cctaagatag acttccatct aaaagattgt ttaggaagaa
     2161 cttggcaatg tggtactatc caattagata tgcaacttcc aagacaattt gataatactt
     2221 atattggtca agatggtgaa aagcatagac cagttatgat tcatagggtt gcttttggta
     2281 gtatagaaag atttataggt atattaatag agcattatgc tggtaaattc ccagtttggt
     2341 tatctccaac tcaagttaag atacttccta tatctgataa atttatggat tatgcaaatg
     2401 aagttaagaa agagttattt gataagggta taagagttga attagatgat agagctgaaa
     2461 aaattggatt taagataaga gaagctcagc ttgaaaaagt gccatacatg ttaatagtag
     2521 gtgaaaaaga agtagctgat aataatgttt ctgttagatc aagagataaa ggtgaaatag
     2581 gtagtattaa attagatgaa tttatagcta gtatttcaaa agaaattgaa tcaagagaaa
     2641 gtattattca agactaatat ataatatatt agtagctaag atattgttat ataagataag
     2701 tttaatcaat atgttgatat gttgatgcat agtgattaaa tttatgaaaa gatgagtata
     2761 attagaggta cagttaatca tatttttatg attaatgtac ctctagtttt ttgtatggta
     2821 gatttattaa taaatatatc atagaagtgt tttgaacatg gaaatattta tgtgcattct
     2881 ttaaataaaa gtttattttt tgagtgataa ttaagtgctt gttggtaagg ttatggctgt
     2941 aaaaatcata actttatttt catatttgtt ccattttcat aaaaattatt atccctttaa
     3001 gaaaatttct taatcctctt gaaatatttc ctttttagtc taaagttaaa tttctttctt
     3061 caaaatgaat atttaaatga actaaaattt aagtacattt tctaatctta tgagtctatt
     3121 tttcatagat aattgcccta taatagaaga atctatgttg gagctatgtg gtgacaataa
     3181 cagcattgtc agtgattaat taatgcttta taaatgatta attagtgttt tatgttattt
     3241 ttggacttac cctctataca tagattaatt tatttaactt tagatataga gagaatgtca
     3301 agtacataca cagacattag agtttaagaa agtgtttatg gagcaagaac ttttaaaaga
     3361 ggtagtgact tagtttaatt tcaagaaatg ataaatacaa aagaggaaaa agagaggtgt
     3421 tgaaatgata gaaaaagtag aagaaaaatc taagattatt aaaacatcaa aagaatataa
     3481 gcgaactata attgaagaaa aagataaaga atggaccaaa aaattaaatg atatacttag
     3541 tagaagtagt ttttctaatg atgaattaga taaaatcaag gcattaattc caaaagagat
     3601 tttgactaat gaaaatgttg gagaagttgt gactgaagat ggatatgcta agcctgagta
     3661 tgatgaggta tttaaatcat tgttcacttt aaataatgaa tatgatttac ttgtaaattt
     3721 tataaatgat atcctaaaag atgctccata tgctaacagg aatatcaaac cgttcactca
     3781 aataaagaga ataattagag ttgaaactga cccaacaatt aattacattg gagagaaaag
     3841 acctagactt gatattttgg ctgaagatga agaaaataat catataaata ttgagatgca
     3901 aagagctttt gagaacgatt atttggaaag agctgaatat tatcttagtc gtgtacatgg
     3961 tagaaagtta gaagaaggta aagagtataa agaaattgga aaaactattg gaatacatat
     4021 actaaatcat gtaaaatata atcacataga agattatgta aattgcctga gattaacaat
     4081 ggacgggcat ccagatattt attctagtaa aactgcatta tattttatag agcttccaaa
     4141 aattcataaa tctgactata tagtaaatag aattatgcta tggggaaaac ttattagtaa
     4201 tccttctaac ttagatgtaa gagttatagc taagaatgat tctattatta aaaaagcact
     4261 tgatagatta aaagaattag gtagcaatga agaatattta ttaaatctga aaagaggtgc
     4321 ttatatcatg aataaaagta gaaattttaa agaagagatt gaaagagaga cagcaattag
     4381 agttgcaaaa gaaaaagatt ataaaataat tataatgttg aaaaaaaatg gttttaaatt
     4441 agatgacata ttaaataaat ttaatgattt tgacttaagt gaagatgaga taaaagaagt
     4501 atatgaaaat aattgatgat aaataaaaag tgaacttata taagtttgct gaatcggagt
     4561 ctggaaatat ttatatgtat tttttaatga atgaaaattt attttaaaaa caataattta
     4621 gaagtacagt taattatatt tttatgatac tgtacttcta gttttttcta ttgtagattt
     4681 attatttact aataaatcta caatagaaat gcttttgttg taagaatcat tttatctaat
     4741 gattgttttt tataatagtt aaatttccat ttgtagtgtt taaatataaa tctataacag
     4801 catttctata aatgttataa atcactataa tgattcaact caaagttact gcttaaagaa
     4861 gcagagattt tatcaaggta tatcatggaa aaaaatattt tattgtaata tttataagat
     4921 tttattatgg aaa
//
LOCUS       DBGJFF010000202         4926 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.207, whole genome shotgun sequence.
ACCESSION   DBGJFF010000202 DBGJFF010000000
VERSION     DBGJFF010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4926
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.207"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(212..1255)
                     /locus_tag="ACOIAF_003662"
     CDS             complement(212..1255)
                     /locus_tag="ACOIAF_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HGZ2670640.1"
                     /translation="MGTKIVLSIVLIVVVVAISLTCIRVIKQSKVGIIMRLGKFQKVA
                     ETGVHFLIPFLDKMAYVIDLREIVIDFPPQPVITKDNVTMQIDTVVYYKVTDPVRYVF
                     EIANPIAAIENLTATTLRNIIGELDLDETLTSRDIINVKMRTILDEATDKWGIKVNRV
                     ELKNIMPPQDIQVAMEKQMRAERERREAILQAEGNKSAAILQAEGEKQSAILTAEAKK
                     EAMVRVAEGEKESAILVAEGEAEAIRQTAIAKAQGEAEMIKRTQIATAEGLKLVFSAM
                     KEADIDNNILALKSMEALEKMAEGKSTKLVLPSEAVNFLGTFKGIKEVMSDDNKEVLD
                     IKEVLNDNESLKK"
     gene            complement(1276..1722)
                     /locus_tag="ACOIAF_003663"
     CDS             complement(1276..1722)
                     /locus_tag="ACOIAF_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HGZ2670641.1"
                     /translation="MKLIWLIVAVLFGIAEMLTPSLTLIWFSVGAVILIFLSSFIESI
                     FLQILIFAVISIAMLVVATKKIVKKDKGYKSNTNLQAMISKKGIVTEEISPNNTGLVV
                     VEHETWTAISIDGEKIEKGSTVEVLKIEGVKLVVKKVDATVNVTNQ"
     gene            complement(1894..3474)
                     /locus_tag="ACOIAF_003664"
     CDS             complement(1894..3474)
                     /locus_tag="ACOIAF_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HGZ2670642.1"
                     /translation="MLIGTSKLSRNVDTACVEEVGIPMIVLMENAVISAMKNMDIDMY
                     NSYTVVCGVGNNGGDGLGIARHLNTYGKEVNVFLVGNLEKLSECSKINYNVLLNMGIN
                     IININKNDLSEKNYGDENEVDKIIKVEKQNIIKNKNLTIFKDRVSKSDVVVDAIFGTG
                     LKRDITGIFKEVIDIVNDNSKNTYSIDIPSGIDSDNGNVLGTCIRANKTISFEFYKRG
                     FLNYDTSTLIGDVVVEKIGVPDFITRKYHDEEFITDKTFVKNNIRRRDKHGFKSDFGK
                     VSIVAGSKGFYGASFIATESAVKSGSGLVTLISSEDVQEKVSIRLTEAMTLNFEEERL
                     NKLLNSSDAIGFGPGMGDNSQTFDKLLKIVENSNCPIVLDADGLNVMKDRCYKFLEWK
                     NRFVITPHLGEMARLTGDSIGYIREHRVDVAKEFAQKYNLVVLLKGYQTVITDGKKTY
                     INPTGNSCMATGGMGDCLLGMITSFIGQGMDILEATVSGAYIHGYIGDKLSKAMYTVN
                     ATDLISNISLTMKELLDI"
     gene            4273..>4926
                     /locus_tag="ACOIAF_003665"
     CDS             4273..>4926
                     /locus_tag="ACOIAF_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2670643.1"
                     /translation="MSGIISVMTQSLILSIMALGVYITYKILDFPDMSADGSYTMGAS
                     IVAFSLTNGISPVVATLMAILCGCTAGLVTGILHIKFKISNLLSGILVMGMLYSINLR
                     IMGKSNIPLFSFKHLFNGEISPIVLALAFVFICKVLLDLFLKTGLGYTLKGVGDNSQM
                     IKSLGINIGSIKILGLMISNGLIALSGSLMAQFLGFSDVNMGIGTLVLGIASIIIGIT
                     "
BASE COUNT         1565 a          846 c          521 g         1994 t
ORIGIN      
        1 atataatatt atataacact ttttacatta gtaaaggata aattgtataa agattctgtt
       61 aatttatttt tataatttca tctttctaat aaaaacaaag aagaagactt aaaaatttat
      121 aagtcctctt cttttcaacg tgcatttttg tgctatattt aattaattcg acctttaatt
      181 tccatatcct tcctatgata acaaaactct tttatttttt taatgattca ttatcattta
      241 gaacttcttt tatatcaagt acttctttgt tatcatcact cataacttcc tttattcctt
      301 tgaatgttcc taggaaatta actgcttctg aaggtaaaac cagttttgtt gacttacctt
      361 cagccatttt ttcaagagct tccatagatt ttaatgctag aatattattg tctatatcag
      421 cctctttcat tgctgaaaaa actaatttta aaccttctgc tgttgctatc tgagttcttt
      481 ttatcatttc agcttcacct tgtgccttag caatagctgt ctgtcttata gcttcagctt
      541 caccttctgc cactaatata gcagattctt tttcaccttc tgctacacgt accatagctt
      601 cttttttggc ttctgctgtt aatatcgcag attgtttttc accttctgct tgaagtattg
      661 cagctgactt attaccttct gcttgaagta ttgcctctct tctttctctt tctgctctca
      721 tttgtttttc cattgcaact tgaatatcct gaggaggcat tatatttttt aactctactc
      781 tatttacttt tataccccat ttatctgttg cttcatcaag gatagttctc atttttacat
      841 ttattatatc tcttgatgtt aatgtttcat ctaaatcaag ttcaccaatt atatttctta
      901 gtgttgtagc tgttaaattt tcaatagcag ctattggatt agctatctca aacacatatc
      961 taactgggtc tgtaacttta taatatacaa ctgtatcaat ctgcatagtt acattatctt
     1021 tagttataac tggttgaggt ggaaaatcta ttacaatttc tctaaggtca attacatatg
     1081 ccattttatc taaaaatggt attaagaaat gtacacctgt ttcagctact ttttgaaact
     1141 taccaagtct cattattata cctactttag attgttttat aactcttata caagttaaac
     1201 ttattgctac gactactact attaatacaa tacttaaaac aattttagta cccatactat
     1261 aaatcctcct ttattctatt ggtttgtcac attaactgtg gcgtctactt ttttaacaac
     1321 taattttacc ccttcaattt ttaatacttc tactgtagaa cctttttcta tcttttcacc
     1381 atctatagaa atagccgtcc atgtttcatg ttcaactaca actagacctg tgttatttgg
     1441 agaaatctct tctgtaacaa tacctttttt agatatcatt gcttgtaaat tagtgttaga
     1501 tttatagcct ttatcttttt ttactatttt tttagtagct acgactaaca ttgctataga
     1561 aattactgca aatattaaaa tttgtaagaa aatactctct ataaagctac ttaaaaatat
     1621 aagtattaca gctcctacac taaaccatat caatgttaag cttggtgtta acatttctgc
     1681 tattccaaat aagactgcta ctatcaacca tattaacttc atactatcta ccctcttttc
     1741 atatgaatta taaaacccta tataaattat atgtttatac aatgcttttg aatataaatt
     1801 taaactacaa atcaatagaa taaactaatc taagattaat ttaatagtat tatatcaaca
     1861 tactttgtta aagttgttac aaagtagtat caattatata tccaacaact ctttcattgt
     1921 taatgaaatg ttacttatta aatccgtagc atttaccgta tacattgctt tgctcaattt
     1981 atctcctata tagccatgta tatatgctcc actaactgta gcttccaata tatccatacc
     2041 ttgacctata aatgaagtaa tcattccaag caaacaatca cccattccac cagttgccat
     2101 acagctattt cctgtaggat ttatatatgt ttttttacca tctgttatga cagtttgata
     2161 accttttaaa agcaccacca gattatattt ttgagcaaat tcttttgcaa catctactct
     2221 atgttctctt atatacccaa ttgaatctcc tgtcaacctg gccatttctc ccaaatgagg
     2281 tgtaattaca aatctatttt tccattctaa aaacttataa catctgtcct tcataacgtt
     2341 caatccatct gcatctaata ctattgggca attagaattt tctactatct taagtaattt
     2401 atcaaatgtt tgactattat ctcccatacc tggaccaaaa ccaattgcat cactagaatt
     2461 aagtaattta tttaatcttt cttcttcaaa atttaaagtc attgcttctg taagtctaat
     2521 agataccttt tcttgcacat cttcactact aattaatgtt acaagtccac tcccactctt
     2581 aacagcagat tcagttgcta tgaaacttgc accataaaat ccttttgaac ctgcaactat
     2641 agatactttt ccaaaatcac ttttaaaacc atgcttatct cttcttctaa tattattttt
     2701 tacaaaagtc ttatcagtta tgaactcttc atcatggtat tttcttgtaa taaaatctgg
     2761 aacacctatc ttttctacaa ctacatcacc tataagtgtg ctagtgtcat agtttagaaa
     2821 acctctttta taaaactcaa agctaatcgt tttgtttgct ctaatacaag ttcctaatac
     2881 atttccatta tcgctatcta tacctgaagg aatatctata gaatatgtat ttttgctatt
     2941 atcgtttact atgtcaataa cttctttaaa aataccagtt atatcacgtt ttaatccagt
     3001 cccaaatata gcatctacta caacatcact tttagatact ctatctttaa aaattgttaa
     3061 atttttattt tttataatat tttgtttctc aactttaata attttatcta cttcattttc
     3121 atctccataa tttttttcac ttaaatcatt tttattaata tttataatat taattcccat
     3181 atttaaaagt acattgtaat ttattttaga gcattcactt agtttttcaa gattgcctac
     3241 taaaaatacg tttacttctt ttccatatgt atttagatgt cttgctattc caagaccatc
     3301 tcctccatta tttccaacac cacatacaac tgtataacta ttatacatgt ctatatccat
     3361 atttttcatt gcagatataa ccgcattttc catcaataca atcattggaa tccctacttc
     3421 ttctacacat gcagtatcaa catttctgct taatttagat gtacctatta gcataaatta
     3481 tcactcctat aaagttaaag acttcttaaa tacttaccaa aaatatctat tgaaatcttt
     3541 ttaatcccat aaatatcata aataaaatca ctaaaactaa gagttttgaa ataatttaaa
     3601 ataacaaaaa attttgaatt aaaacaatta tttaatttat tataccatat aatttaagtt
     3661 attatgttgg tttaaaacct atttacacta attatctaaa tatctcttta tttttataat
     3721 cttttatgac ttatttatat ttttcatttt tataaaaaaa caataattaa gaatataact
     3781 atattctaca tgtaaaaatt tttttattta aactcaattt tctgataatt tgataatttt
     3841 tcaaaattta tagttgactt ttatttttta ctgtattaaa ctaattacta tacaaaacag
     3901 aaaacaaaat tttcaaaatt attaaattaa aaggagaggt aaatatgaag actttattaa
     3961 acaaacaagt acgatttttt agtagccaat ttagttaggg tttgtaccta agttaagaca
     4021 aattgtcata acatagatac aagcccctta ggattatgat ttttaaggat tgtatctatg
     4081 ttggctatag taattgttgt taaagttttt attttatcat cataatatag agccacatgg
     4141 attatactgt gtggcttatt cgaatttttg ccatacagtt atatatactg tgtggttttt
     4201 ttatatcaaa atcatttttt aaaaccaaat ttaaaaaacc atttaaaata ttttcaaagg
     4261 ggtgttttat ttatgtcagg tattatatct gtaatgacac aaagtttaat tttatcgatt
     4321 atggcactgg gggtttatat aacttacaaa atcttagatt ttccagatat gtctgctgat
     4381 ggtagctata caatgggtgc ttctattgta gcattttctc ttactaatgg tatttctcct
     4441 gtagtagcaa ctttaatggc tatcttatgt ggttgtactg ctggtcttgt tacagggatt
     4501 cttcacatta agtttaagat atctaattta ctttctggta ttttagttat gggaatgcta
     4561 tactctatca atttaagaat aatgggaaaa tcaaatattc ctctatttag ctttaaacac
     4621 ttattcaatg gggaaatatc tcctatagtt ttagccttag cttttgtatt catctgtaaa
     4681 gtccttttag atttattcct aaaaacaggt cttggataca ctttaaaagg tgttggtgat
     4741 aactctcaaa tgattaaatc cctaggtata aatataggtt ctataaaaat actaggactt
     4801 atgatttcaa atggacttat agctttatct ggtagtctta tggctcaatt cttgggattc
     4861 tcagatgtaa atatgggtat aggaacttta gttcttggaa ttgcctctat cataattggt
     4921 attaca
//
LOCUS       DBGJFF010000203         4890 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.208, whole genome shotgun sequence.
ACCESSION   DBGJFF010000203 DBGJFF010000000
VERSION     DBGJFF010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4890
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.208"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            224..730
                     /locus_tag="ACOIAF_003666"
     CDS             224..730
                     /locus_tag="ACOIAF_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HGZ2670644.1"
                     /translation="MGFREVKIEELQFNPFTKIGKEWLLITAGDSEKFNTMTASWGGV
                     GVYWGKNVVTIYIRPQRYTKEFVDSNDTFTVAFFDETYREALNICGTISGRDINKIEK
                     AGLTPYFVDDTVAFEEANMIIVCKKLYHDNMPPENFDAKENDKKWYPEKDYHTMYISE
                     IIKVLVKE"
     gene            1143..1811
                     /locus_tag="ACOIAF_003667"
     CDS             1143..1811
                     /locus_tag="ACOIAF_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MORN repeat-containing protein"
                     /protein_id="HGZ2670645.1"
                     /translation="MENITSYFTTILCVFICLSSVFIFTQLARVFINKKKINQKIKSR
                     NGFRYDRDFIEARREEIHIKDNNNNKNKSNNKKLKEEKVFKYDNGDLYKGEFVDGKKN
                     GFGIYIFSSKEKYEGLWKDDKMHGIGKYTYRDGSIYTGEFKYGLKNGLGKLTYPNNDI
                     YKGYFLDNKKNGKGVLYKNDGNKQAGIWEDDEQCKSLDFKDLNNNKINNVYQNRLNNN
                     KLKV"
     gene            1970..2476
                     /locus_tag="ACOIAF_003668"
     CDS             1970..2476
                     /locus_tag="ACOIAF_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HGZ2670646.1"
                     /translation="MKEYVVVDLETTGLDPYKGCEIIEIGITEIKNEQIVKNYSRLIK
                     PKGIISSFITELTHISNEMVENEEPLELVLPRFRKYIGDRTIIAHNAKFDLKFLNYYL
                     RMLDLEPINNYICTVELLKKCKSYKGKNKKLETACAYYNIENINAHRADSDTLATAKL
                     FLKIKDEY"
     gene            2604..3620
                     /locus_tag="ACOIAF_003669"
     CDS             2604..3620
                     /locus_tag="ACOIAF_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HGZ2670647.1"
                     /translation="MGYYNDNDNNKIRRKKISNNKDSNKKSKHSYTSANKKNKSPNTS
                     SSKRMSEKVDSQNLIKRDRDSFTRDNVRPLNTDYNQRERRRVEYKQREKKLKSKKRKK
                     QKRIRIVRALLSIMLVALIIFSVAGAIFTVSAIKGSPEVTKKLIRSKYISSEVVSIKQ
                     MPNDLKHAIVSIEDERFYKHKGVDVISLVRSVLNNVFTDTTQGASTIDMQVSKNLLTS
                     NDKTMKRKIRDMYNAKQMNKIMTKDEILEAYLNNMYLGKNAYGAAKGAEVYFGKKVSE
                     LNLAECAMLAGMTNNPARYIDHGEAKKRQVIVLYKMHQLGYITDKEYRIAKADDTPFK
                     SEID"
     regulatory      3816..3910
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00442"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="guanidine-I (ykkC/yxkD leader) riboswitch;
                     Guanidine-I riboswitches control genes whose products are
                     involved in modification or pumping out guanidine as a
                     toxic compound from bacteria; This is class of guanidine
                     riboswitches is based on the ykkC/yxkD leader; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="guanidine"
                     /db_xref="RFAM:RF00442"
     gene            3974..4678
                     /locus_tag="ACOIAF_003670"
     CDS             3974..4678
                     /locus_tag="ACOIAF_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B3/4 domain-containing protein"
                     /protein_id="HGZ2670648.1"
                     /translation="MKKFVIEDAFWNLFPSAKIGVVICNDIDNSIKDEDYKKIILKGE
                     EEALKYLEDSEFSNNQVIKVWREAFKKFKTKKGARSSIEALLKRVHNGNNIGTINPLV
                     DIYNFISLKYALPCGGEDIDKFIGDIRLTRAIGNEEFIPLGTDENSFPYEGEIIYKDD
                     GGAICRCWNWREAVRTMLTEDTNNAFLCIELVDESRFKEFENALNELAKIVQEKLGGN
                     CKISILDIDNKEVSVD"
BASE COUNT         2075 a          419 c          811 g         1585 t
ORIGIN      
        1 tttaggttta aaaatatatt atatacttta taatcaatat attttatcta tattttacac
       61 ttataaaata tgaaaactac ttattgcgaa agtaattgat ttgttgtatt ataatttaca
      121 tgaattatgt aggacaatca aaagtcagta ttataagagt acatatattc aaatttttaa
      181 caattaattt atataattaa aaaatgaagg gtaggatgaa gtcatgggtt ttagagaagt
      241 aaaaattgag gaattacagt ttaatccatt tactaagatt ggcaaagaat ggttacttat
      301 aacagctggt gattcagaaa agtttaacac aatgactgct agctggggtg gtgttggagt
      361 atattggggt aaaaatgttg ttacaattta cataagacca cagagatata caaaagaatt
      421 tgtagatagt aatgatacat ttacagttgc cttctttgat gaaacgtata gagaggcttt
      481 aaatatttgt ggtactattt ctggtagaga tataaataaa atagaaaaag caggacttac
      541 accatatttt gtggatgata cggtagcttt tgaagaagct aatatgatta tagtatgtaa
      601 aaagctatat catgataata tgccaccaga aaactttgat gctaaagaaa atgataaaaa
      661 atggtatcca gagaaagact accatactat gtacatttca gaaattataa aagttcttgt
      721 aaaagaataa ttataataaa aaatatatga aaagaactta ttataagtaa atgactgtgc
      781 tttcttatct gatatctaag tagatgctag taactaaata aatattaatt ttaaataaat
      841 actattataa aaaagatgaa ctatatttac tatagttcat ctttttattt atataagcac
      901 ttatataagt agagatttat ttgaataaaa gtatttattt gaaatatttg agtatacttt
      961 ataagtattt ttatgctaac tagaaacact taatttatgg catttgtatt tgatttattt
     1021 taatgtaaat aatttcaaag ttaattttat tcaatatagt actataaaag tataaataac
     1081 atttaataaa aatatatatt atattaggta ttttaaataa attttttaaa gggtgatttt
     1141 ttatggaaaa tataactagt tattttacaa caatactatg tgtatttata tgcttatcat
     1201 ctgtatttat atttactcaa ctagctagag tatttattaa taagaagaag attaatcaaa
     1261 aaattaaatc aagaaacgga tttaggtatg atagagattt tattgaagcc agaagagaag
     1321 aaatacatat aaaggataac aataataata aaaataagag taacaataag aaattaaagg
     1381 aagaaaaagt atttaaatac gataatggtg atttatataa aggtgagttt gttgatggaa
     1441 agaaaaatgg atttggaata tatatttttt caagtaagga aaagtatgaa ggattatgga
     1501 aagatgacaa gatgcatgga ataggaaaat atacttatag agatggttca atatatacag
     1561 gtgaatttaa atacggatta aaaaatggtt tgggaaaatt aacatatccc aataatgaca
     1621 tttataaggg atatttttta gataataaga aaaatggtaa gggtgtatta tataaaaatg
     1681 atggaaataa acaagcagga atatgggaag atgatgaaca atgtaaaagt ttagatttta
     1741 aagatttaaa taataataaa ataaataatg tttatcaaaa tagattaaat aacaataaat
     1801 taaaagttta attattctaa taaaaaaatc tttatttatg atggaatata ggatatatct
     1861 atgttgtaat ttgtaaaatc ttcctatatc atataaaatt aagtattgat tagtctatta
     1921 cataaatata gaatataaat atatccattt aataataagg agaagagata tgaaagagta
     1981 tgtagttgtg gatttagaaa ctacaggatt agatccatat aaaggttgtg aaataataga
     2041 aataggaata acagaaataa aaaatgaaca aattgtaaaa aactattcaa ggttgataaa
     2101 acctaagggg ataatttctt catttataac agaattgaca catataagta atgaaatggt
     2161 agaaaatgaa gaaccactag aattagtttt acctagattt agaaaataca taggtgatag
     2221 aactataatt gcacacaatg cgaaatttga tttaaaattt ttaaattact atcttagaat
     2281 gttagattta gaaccaataa acaattatat atgtacagta gagttattaa aaaaatgtaa
     2341 aagttataaa ggaaaaaaca agaaacttga aacagcttgt gcttattaca acatagagaa
     2401 tataaatgct catagagctg acagtgatac attagcaact gcaaaactat ttttgaaaat
     2461 aaaagatgaa tattaattat aaatatagag aaaattagtg aaaatatcct ttataaaatt
     2521 gacccttaag cttacaattt tgttctttga tttattttgt agaacaatgt ataatgtacc
     2581 ttatatgaag aagggagact aaaatgggtt attacaatga taatgataat aataaaataa
     2641 gacgtaaaaa aattagtaac aacaaagata gtaataaaaa atctaaacat tcttatactt
     2701 cggcaaataa aaaaaataaa agtcctaata caagttccag taaaagaatg agtgaaaaag
     2761 tagattcaca gaatcttata aaaagagata gagatagttt tacaagagat aatgtaagac
     2821 ctttaaatac agattacaat caaagagaaa gaagaagagt cgagtataaa caaagagaga
     2881 aaaaattaaa aagtaaaaaa agaaaaaaac aaaaaaggat tagaatagtt agggcattat
     2941 tatctattat gttagttgca ctaattatat tttcagtggc aggtgcaata tttacagttt
     3001 cagctataaa agggtcacca gaagttacta aaaagttaat taggtctaaa tatataagta
     3061 gtgaggttgt aagtataaaa caaatgccaa atgatttaaa acatgcaata gtttccatag
     3121 aagatgagag attttataag cataaaggag tagatgttat ttctctagtt aggtcagtat
     3181 taaacaatgt gtttacagat acaacacaag gtgcaagtac tatagatatg caagtttcaa
     3241 aaaacctgct tactagtaat gataaaacta tgaagcggaa aataagggat atgtataatg
     3301 ctaagcagat gaataagatt atgactaagg atgaaattct tgaagcatat ttaaataata
     3361 tgtatctagg taaaaatgca tatggagctg caaagggagc tgaagtctac ttcggtaaaa
     3421 aagttagtga attaaattta gctgagtgtg ctatgttagc tggtatgaca aataatcctg
     3481 caaggtatat tgaccatgga gaggctaaga agagacaagt gattgtactg tataagatgc
     3541 atcagcttgg ttatataact gataaggagt atagaatagc aaaagctgat gacactccat
     3601 ttaaatcaga aattgattaa aattgtaaga atatagtatt ttcaatagtt taaacaaacg
     3661 aaaatgtaat aaaaaaggtg atttgccacc catttgccac cataattata atttaatagc
     3721 aaattgtttt aatgtacaaa tcagataata taatattgtt ttaaatacta cattatgcta
     3781 aaatttaatt gtattttgta aattaaaaaa cttataattt agttttctag ggttccgcat
     3841 tttataatgg tcaggtccaa gagaaaacgc acaattgttg tgtacacgga aggataaaag
     3901 cctgggagat atttaataat atttctatag gcttttttat ttttacatat tttaaatttg
     3961 aggaggtttt ctaatgaaaa aatttgttat tgaggatgca ttttggaatc tatttcctag
     4021 tgcaaaaatt ggagttgtta tttgtaatga tatagataac tctataaaag atgaagacta
     4081 taagaaaata attttgaagg gagaagaaga agcattaaaa tacttggagg actcagaatt
     4141 tagcaacaac caagtgatta aagtatggag agaagcattt aaaaaattta aaacgaaaaa
     4201 aggagcaagg tcttctattg aagcattatt aaaaagagtt cataatggaa ataatatagg
     4261 aactattaac cctttagtgg atatctacaa ttttatctca ttaaaatatg cattaccttg
     4321 tggaggggag gatatagata aatttatagg agatattagg ctaactagag caattggtaa
     4381 tgaggagttt atcccactag ggacagatga aaattcattt ccatatgaag gagaaataat
     4441 ttataaagat gatggtggag caatttgtag atgttggaat tggcgtgaag cagtgagaac
     4501 aatgcttact gaagatacta acaatgcctt tttatgcatt gaattagtag atgaaagtag
     4561 atttaaagaa tttgaaaatg ctttaaatga attagctaaa atagttcaag aaaaattggg
     4621 tgggaattgt aaaatttcaa ttttagatat tgataataaa gaggtatcag tggattaata
     4681 tggaggtgct gttgtaatgg actattctag ttataaagat acttatattt agggtaagaa
     4741 ggtctatttt agatataacg attatcaaag gatataatat agaaaataaa aatgtagaat
     4801 ataaaaagcc tttaaaataa gaaaaaaatg tattttaaag gcttttatta tactcttttt
     4861 tatttgcata attaacaata aaaatatatt
//
LOCUS       DBGJFF010000204         4869 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.209, whole genome shotgun sequence.
ACCESSION   DBGJFF010000204 DBGJFF010000000
VERSION     DBGJFF010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4869
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.209"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            516..1376
                     /locus_tag="ACOIAF_003671"
     CDS             516..1376
                     /locus_tag="ACOIAF_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HGZ2670649.1"
                     /translation="MKIIQILNNNVALVKRGKNEIIVFVKGIGFKKRVGQVINENEIE
                     RTYILDSYDMLEHFSYLLQNSDSNNIVLINEIIEYGEKALNIKASDYLSLTLLDHLEF
                     LLKRCEKNQFIKSPLVWNVKRFYPKHFEIGMHALKLIGDSKGINLPDDEAVSIALHFV
                     NMEVNKESHDSTIVELRTLADIVSIIKYHFNVELDETSTNYMRFTTHLQYFIQRIMSD
                     EASIDEDNSIDLYKQVSQLYPKSFGSVQKIRKYIKSQFNVDISISEETYLMLHINRVT
                     QRLEAKSDEI"
     gene            1366..3237
                     /locus_tag="ACOIAF_003672"
     CDS             1366..3237
                     /locus_tag="ACOIAF_003672"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HGZ2670650.1"
                     /translation="MKYKDLNENIIKLIGGKENIKSVAHCVTRLRFTLNDRSIAQTEE
                     IKKLKGVIDVVSNDVAYQIIIGTHVVDVYNEFMSMIGLSSSESSIKSERKKGIRGIKG
                     IFNSIFVVVSETMTSVIEVLLAAGILASMLALLNMSGILQSDSPTYQILDTLRSATFH
                     FLPVLIAIASAKRLNVNPYIAVVLAVTLLSSSIDGVEGLSLFGINLQATTYANSFIPI
                     LLGVWAMGIIIEGLKKILPKALHYFLVPVLSLIITLSVTLTVFGPIGMWIGDGLNYAC
                     MFLIDTLGNWSVVALYAALQPLLIVFGAANFTMPISLNSVTTLGYDPIFMGAATISDI
                     AVCGAMFGYFLKTRNKEQKQLFGTVSFSALMGVTEPSVFGVFMKYRRPFVAVMIGGGI
                     GGLIAGLAQVKTYGMVWGLAALPTYLVGGEVSNFTFMIISVVVSFVAATAASYLLGIP
                     SDEKEEVKGDNEKELELIINENNSRTISIGNVAKGQVIALGEVNDKAFSSGALGKGVG
                     VIPSESKSTVISPVDGTITIVFPTKHAYGIVTDEGLEILIHIGIDTVNLDGKFFESLV
                     TQNQRVKKGDALAVFESDKIIEEGFDSTIITVVTNTSDYLDVISSNDESEELLTVIV"
     gene            3257..4711
                     /locus_tag="ACOIAF_003673"
     CDS             3257..4711
                     /locus_tag="ACOIAF_003673"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HGZ2670651.1"
                     /translation="MNIKDTFFWGGSIAAHQCEGSWDSDNKGPAIMDFVTKGSYETPR
                     VITDKIEEKLDYPSHNGIDFYNRYKEDIALFKEMGFSALRISIDWSRIFPNGDDENPN
                     ELGIKYYEGLIDTLIENNIEPIVTLYHFELPMNLVHKYGSWNNRKLIDLYLKYSETVI
                     RRFDDKVKYWVTFNEMNHIDPQTEHSDIFTYMIAGIKYSEIENKIQTLANVGYNMTLA
                     SVKVAKLAREINPNNKVGCVFGLNPIYPYNCNPSNVLNAFLDNDRDYYQIDAMCNGKF
                     PQYKLKEYEKNNITIEVTDEDRKAFKEGKLDFIGLNYYFSSVSTPKENLSGDKSLFGG
                     IQNPYLEQSKWGWAIDPIGLRFTLNYIYRKYQLPILITENGLGAYDKIEEDGTINDDY
                     RIEYLSKHLSQMKSAIEEDNVECFGYLMWGPIDLVSATTGQMSKRYGFIYVDLDDEGK
                     GSFKRYKKKSFDWYKEVIKSNGEVLDFSTDIVVG"
BASE COUNT         1867 a          509 c          897 g         1596 t
ORIGIN      
        1 tatttatata ttagtatatc ttactagcat ttaaatgaaa ctaaatttga agctttacct
       61 attaatatat cttaatagtg attaaataaa actaaactta agactttata tgttaatata
      121 tcttaagaat ttatttgtta acatacagtt cattatattt aaaagcatta ctatgataat
      181 tcataatagt aaaatagata atggttcaga caatattata aatattagtg acggtaaaat
      241 agtatcaatt tagtatataa atcttgtcag ataatcgcat aacagagctt gtagttatag
      301 aataaaaaaa tatctaggaa accgttgaca gatttatatg gatagtataa catatagaat
      361 atgaaattaa acaaaaaaga attttaatta tggcgatgta gtcataattt tgagtatgtt
      421 actgattagc aggcagaact caagtatata tgtgaaaaat agagggatac tctatacata
      481 tatgcttgag ttctttttat tgaagggagg aatatatgaa aataattcag attttaaata
      541 ataatgtagc cttagtaaaa agaggtaaaa atgaaattat tgtatttgtt aaaggaattg
      601 gctttaaaaa aagagtggga caagtaataa atgaaaatga aatagagaga acatatatac
      661 tagattcata cgatatgcta gaacacttta gttatctact acaaaactca gactccaata
      721 atatagttct tattaatgaa ataattgaat atggtgagaa agctttaaat ataaaggcaa
      781 gtgattatct aagcttaacc ttactagacc atttagagtt tttacttaaa agatgtgaaa
      841 aaaatcaatt tataaaaagt cctttggtat ggaatgttaa aagattttat ccaaagcatt
      901 ttgaaattgg aatgcatgca ctaaaattaa ttggtgatag taagggaatt aatcttcctg
      961 atgatgaggc agtatcaata gcacttcatt ttgtaaatat ggaggtaaat aaagagtctc
     1021 atgatagtac aattgtagag ttgagaactt tagcagatat agtttctata ataaaatatc
     1081 attttaatgt agagctagat gaaacttcaa caaattatat gagatttact actcatctac
     1141 agtactttat ccaaagaatt atgtcggatg aagcttcaat agatgaagat aattctatag
     1201 atttatataa acaagtaagt caactttatc caaagtcatt tggaagtgtt caaaagataa
     1261 gaaaatatat aaaatcacaa tttaatgtcg atatatctat aagtgaagaa acttatttaa
     1321 tgttgcatat aaatagggtg actcaaagac tggaggcaaa atcagatgaa atataaagat
     1381 ttaaatgaaa atataataaa acttataggg ggaaaagaaa atataaagtc ggtagcgcat
     1441 tgtgttactc gtttaagatt tactttaaat gataggagta tcgctcaaac agaagaaatt
     1501 aaaaaattaa aaggagtaat tgatgtagtt tctaatgatg ttgcttatca aattataata
     1561 ggtactcatg ttgtagatgt atataacgag tttatgtcta tgataggttt aagttcgagt
     1621 gaatcaagta taaaaagtga aagaaaaaag ggaattagag gtatcaaagg tatatttaat
     1681 tccatctttg tggtagtctc agagactatg acttcagtaa ttgaagtact acttgcagct
     1741 ggtatacttg caagtatgtt agcactttta aatatgtctg gcatacttca gagtgatagt
     1801 cctacatatc aaatattaga tacattaaga agtgctacat ttcacttctt accagtatta
     1861 attgcaatag catcagcaaa aagacttaat gtaaatccct atatagcggt agtgttagca
     1921 gttacgttat tatcttcatc aatagatggt gtagaaggat taagtttatt tggtataaat
     1981 ttacaggcta caacttatgc aaacagtttt attccaatac ttttaggagt atgggcaatg
     2041 ggaattataa ttgaaggttt gaaaaaaata ttacctaaag cactacacta ttttttagtt
     2101 ccagtattat ctttaataat aactctatca gttacattaa ctgtatttgg acctattggt
     2161 atgtggattg gtgatggatt aaactatgca tgtatgtttt taattgatac attgggtaat
     2221 tggagtgttg ttgcactgta tgcagcttta caaccactct taatagtttt tggtgcagca
     2281 aactttacta tgcctatatc tttaaattct gtaacaacat tggggtatga cccaattttt
     2341 atgggcgctg caacaatatc tgatattgca gtttgtggtg caatgtttgg atatttctta
     2401 aaaactagaa ataaagaaca aaaacaatta tttggtacag taagttttag tgcacttatg
     2461 ggagttacag aaccttcagt gtttggtgtg tttatgaaat atagaagacc atttgtagca
     2521 gtaatgatag gtggaggtat aggtggttta atagcaggtt tagctcaagt taaaacatat
     2581 ggaatggttt ggggattagc tgcattacca acgtatcttg ttggaggaga ggtatctaac
     2641 tttacattta tgataataag tgtagtagta tcatttgtag cagcaacagc agcttcctat
     2701 ttactaggaa taccttcaga tgaaaaagaa gaagttaagg gtgataatga aaaagaatta
     2761 gaactaataa tcaacgagaa caattcaaga actattagta ttggaaatgt tgcaaaaggt
     2821 caggtaattg cacttgggga agttaatgac aaagcatttt cctctggagc attaggtaaa
     2881 ggggttggag ttatacctag tgaatcaaaa tcaactgtta tatctcctgt agatggaact
     2941 ataactatag tatttccaac taaacatgca tatggtatag tgactgatga aggattagaa
     3001 atattaattc atattgggat agatactgta aacttagatg gaaaattctt tgaaagtctt
     3061 gtaacacaaa atcaaagggt taaaaaagga gatgctttag cagtatttga atctgataaa
     3121 attatagagg aaggctttga ttctactata ataactgtag ttacaaatac tagcgattat
     3181 ttagatgtaa tctcatcaaa tgatgaatca gaagaattat taactgtaat agtataaatt
     3241 ttaggaggga aaattcatga atattaaaga tacatttttc tggggaggaa gtattgcagc
     3301 ccatcaatgt gaaggttcat gggatagtga taacaaagga cctgcaatta tggattttgt
     3361 aactaagggc tcttatgaaa cgccaagagt aattactgat aaaatagaag aaaaattgga
     3421 ttatccatct cataatggaa tagattttta taatagatat aaagaagata tagctttatt
     3481 taaagaaatg ggatttagtg cattaagaat atctatagat tggtcaagaa tttttccaaa
     3541 tggagatgat gaaaatccta atgaattagg gattaaatac tatgaaggtc tgatagatac
     3601 attaatagaa aataatatag aacctattgt aacactatat cattttgaat tacctatgaa
     3661 tttagtacat aaatatggtt catggaataa cagaaaactt atagatttgt atttaaagta
     3721 cagtgagact gtaataagaa gatttgatga taaggtaaag tactgggtta catttaatga
     3781 gatgaaccat attgaccctc aaacagagca ctcggatatt tttacttata tgatagcagg
     3841 tattaagtat agtgaaatag aaaataagat tcaaacattg gcaaatgtag ggtataatat
     3901 gactttggcg agtgtaaagg ttgcaaagtt ggctagagaa atcaatccca acaataaagt
     3961 tggatgtgta tttggactaa atccaattta tccatacaat tgtaatccta gtaatgtgtt
     4021 aaatgcattt ttagataatg accgtgatta ttatcaaatt gatgcaatgt gcaatgggaa
     4081 gtttccacaa tataaattaa aagaatatga aaaaaataat ataactattg aagtgactga
     4141 tgaagacaga aaagcattta aagaaggtaa gttagatttt ataggactta attattattt
     4201 ttcaagtgta tctactccaa aagaaaattt atcaggtgat aaatctttat ttggtgggat
     4261 tcaaaatcca tatttggaac aaagtaagtg ggggtgggca atagacccta taggcttaag
     4321 atttaccttg aactatattt acagaaagta tcaattacct atcttaatta cagaaaatgg
     4381 tttaggggca tatgataaaa tcgaagaaga tggaactata aatgatgatt atagaattga
     4441 atatttaagt aagcatctat ctcaaatgaa gtctgcaatt gaagaggata atgttgaatg
     4501 ttttggatat ttgatgtggg gaccaattga tttagtcagt gcaactacag gtcagatgag
     4561 taagagatat ggatttatat atgttgactt agatgatgaa ggcaaaggaa gttttaagag
     4621 atataagaaa aaatcatttg attggtacaa agaagtaata aaatctaatg gagaagtttt
     4681 agatttttct acggatatag tagtagggta ggaataagat aaatagaagt taggaactaa
     4741 tgttaattag tgtctagtaa agcatttaaa atagagtata gggtctttaa agtaaagtgt
     4801 gtattcttct taaaagtaat ggcaactaaa attactataa aaaggagaat acacattttt
     4861 tatgtttat
//
LOCUS       DBGJFF010000205         4856 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.210, whole genome shotgun sequence.
ACCESSION   DBGJFF010000205 DBGJFF010000000
VERSION     DBGJFF010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4856
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.210"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            10..813
                     /locus_tag="ACOIAF_003674"
     CDS             10..813
                     /locus_tag="ACOIAF_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670652.1"
                     /translation="MEKLRVIEMIKNNPNIIATIDNPTDEMKLLAIKENGLVLEYINN
                     PTREMQELAIDNNIRAIKFIDNPTEDMMIKAVNEGWSILDYIKNPTDKVIEMAINQAG
                     WAIKYAKNPSEELQLLAVRKNYDSIKFIKQPYESVQEEAVRISYDALRYIDSPSYNAE
                     LIAIKNNEAAISFITDLDKNKMLEFLKVNILVIKYILKKVSKDELESVLKEVLSKEDV
                     EEKYIRDFLNCSVIDRNSESIPIDKIMFIYKYGSKTAKKIAVDEKLKIM"
     gene            825..1955
                     /locus_tag="ACOIAF_003675"
     CDS             825..1955
                     /locus_tag="ACOIAF_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGZ2670653.1"
                     /translation="MNFIDTLKSVDLILSTGEVPLIVGESGIGKTALANKLAKENDWS
                     LIVIDGNLLKEGEIGGLPTIESYVGVNSNGYKTEKKTTVYAVHNKLREIDEEISRSKT
                     VLLFIDEINRCEHTVQQELMNLILNREINGYKLHDDVKILAAMNPSSKYGSDFDYQVV
                     DMDAAQENRFVWLNMESDHTQWIKWAIDEGIERKVIEFISTFPEYLHKINEDDVRATP
                     RSYERVSKIYKVYKEKNNSIPRAVFLNVVKGNVGKVIAEEFISFIESNSEPLISYEDV
                     FLGESIDESIVERVKNESHTRLYLSAMNILKDLELNIKNDKYESNHYINRFIEFLKMY
                     PVDLMIGIMKDIRNSYIEVYKKAIENEEFVKSYFESYSLIRG"
     gene            1959..3368
                     /locus_tag="ACOIAF_003676"
     CDS             1959..3368
                     /locus_tag="ACOIAF_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA-like domain-containing protein"
                     /protein_id="HGZ2670654.1"
                     /translation="MENYFDKQAKELYNKASEIINTHSMLKANRANEKFDIDIPQDFK
                     YEFFSLVDKVNLSLMEEEDNFYGYFLFQMSREIRFDISSPTGVNFKGAKYIIYFNPII
                     FLTLDIKQMETTIKHEIHHILSMHLMRAKELKGKYSTLAINMAMDIVVNKFLNNLPPY
                     ATTLEWVNLKYSLKLEPYEPFEYYVEKIQTELDLMEVDEDGEEDDSDKYGDIEIDYSP
                     ERTHDIWDESNEIDENTLREFTEKFINSSQKGKVPAYLESMISSLKNSKGELPWNLYL
                     NRIMGTVENNKKKTITRRNRRQPNRLDLRGELRSHKAEIAVALDVSGSISDEEFKQAI
                     KEVLSIVKNYNHEITIIECDNEIRRVYKVRTEKDIKDRIRIGGGTRFSPVFEYANNKK
                     INLLVYFTDGKGENKLQVIPRGYKILWVISGRGDKISLKEPYGAVKKLTKVKTNDEIL
                     EINDVRNDGYSMNSQAPIL"
     gene            3585..4088
                     /locus_tag="ACOIAF_003677"
     CDS             3585..4088
                     /locus_tag="ACOIAF_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HGZ2670655.1"
                     /translation="MFLFKIDDYIMYGMTGVCKVMDITNERFTNGIKKEYYVLSPIYS
                     NNTVIKIPVDNEKVPMRKLLSKINVLSLINDIPNMDTSWIDNEKLRSEQFKKILRGGK
                     CEELIKLVRSIDNNREYVKSIGKKTHQADDNIMKEAERLLNEEFATILDISPNEVSSY
                     ISSHIPQ"
BASE COUNT         2013 a          410 c          851 g         1582 t
ORIGIN      
        1 aggcacatta tggaaaaatt gagagttatt gaaatgataa aaaataatcc caatattata
       61 gctacaatag ataatccaac tgatgaaatg aaattgttag ctattaaaga aaatgggctt
      121 gtactagaat atataaataa tccaaccaga gagatgcaag aactagcaat agataataat
      181 attagagcaa ttaaatttat agataatcca acagaagata tgatgataaa agcagtaaat
      241 gagggatgga gtatactgga ttatatcaaa aacccaactg ataaagttat tgaaatggct
      301 ataaatcaag caggatgggc aattaaatat gctaaaaatc caagtgaaga attacaactg
      361 ttagcagtaa gaaaaaatta tgattcgata aagtttataa aacaacctta tgaaagtgta
      421 caagaggagg cagtgagaat aagttatgat gccttgagat atatagattc acctagttat
      481 aatgcagaac ttatagctat aaaaaataat gaagcagcta ttagctttat aactgattta
      541 gataaaaata aaatgttgga atttttaaaa gtgaatattt tagtgattaa gtacattctg
      601 aaaaaagtat ctaaagatga attagagagt gtattaaaag aagtattatc caaagaagat
      661 gttgaggaaa agtatattag agattttttg aattgtagtg ttatagatag aaatagtgaa
      721 agtataccaa tagataaaat tatgtttatt tataaatatg gaagtaagac agcaaaaaaa
      781 atagctgtag atgagaaatt aaagattatg taggagagag aaaaatgaat tttatagaca
      841 cattaaaaag cgttgactta atattatcca ctggagaagt acctttaata gtaggtgaaa
      901 gtggaatagg aaaaacagct ttagcaaata agcttgccaa agaaaatgat tggagtttaa
      961 ttgttattga tggaaactta cttaaagagg gtgaaatagg tggtcttcca actatagaat
     1021 cttatgtagg agttaattct aatggatata agactgaaaa gaaaacaaca gtgtatgctg
     1081 ttcataacaa attaagagaa attgatgaag agatatctag aagtaagaca gttcttttat
     1141 ttatagatga gataaatcgt tgtgagcata cagtacagca agaacttatg aatttaattt
     1201 taaatagaga aattaatgga tataaactgc atgatgatgt aaagatttta gcagcaatga
     1261 atccatcaag taaatatggt tcagattttg attatcaagt ggtagatatg gatgcagccc
     1321 aagaaaatag gtttgtttgg ttaaatatgg agagtgacca tacacaatgg ataaaatggg
     1381 caatagatga gggtattgaa agaaaagtta tcgagtttat ttccactttt ccagaatatc
     1441 tgcataaaat aaatgaagat gatgtaagag ctactcctag aagttatgag agagtatcta
     1501 aaatttataa agtttataaa gaaaaaaata attctattcc tagagcagta tttttaaatg
     1561 ttgtaaaggg taatgtaggt aaagttattg cagaagaatt tataagtttt attgaatcaa
     1621 actctgaacc attaatatcc tatgaagatg tttttttagg agaatctatt gatgagtcta
     1681 ttgtagaaag agtaaaaaat gagagtcata caagacttta tctatcagct atgaacattc
     1741 taaaagactt agaattaaat attaagaatg ataaatatga atcaaaccac tatattaata
     1801 gatttattga atttttaaaa atgtatcctg tagatttaat gataggtatt atgaaggata
     1861 ttagaaacag ttatattgaa gtatataaaa aagctataga aaatgaagag tttgtaaaat
     1921 catattttga atcttatagt ttaataaggg gataatgtat ggaaaattat tttgataaac
     1981 aagcaaaaga gctttataat aaagcaagtg aaattataaa tactcattct atgctaaagg
     2041 caaatcgtgc taatgagaaa tttgatatag atataccaca agattttaag tatgagtttt
     2101 ttagtcttgt agataaagtg aatttaagtc ttatggaaga agaagataat ttttatggat
     2161 attttttgtt tcagatgtca agagaaataa gatttgatat aagtagtcct acaggtgtga
     2221 attttaaggg agctaaatat attatatatt ttaatccaat aatcttttta acgcttgaca
     2281 taaagcaaat ggagactaca attaaacatg aaattcatca tatattatct atgcatttga
     2341 tgagagcaaa ggagttaaaa ggaaagtata gtacattagc aattaatatg gcaatggata
     2401 tagtagtaaa taaatttcta aacaatttac caccatatgc aactacctta gagtgggtaa
     2461 atttaaagta ttccttaaaa cttgaaccat atgaaccttt tgaatattat gtagaaaaga
     2521 ttcagactga attagatttg atggaagtgg atgaagatgg tgaggaagat gatagtgata
     2581 aatatggaga tatagaaatt gattatagcc ccgaaagaac tcatgatata tgggatgaat
     2641 ccaatgagat agatgaaaat acccttagag aatttactga aaaatttatt aacagttctc
     2701 aaaaaggtaa agttcctgct tatttagaaa gtatgatatc atcacttaaa aatagtaagg
     2761 gtgaattgcc atggaattta tatcttaata gaataatggg aacagttgaa aataataaaa
     2821 agaagactat aacaagaaga aatagaagac aaccaaatcg tttagatttg aggggagaac
     2881 ttagaagtca taaggcagaa atagcagttg cacttgatgt aagtggaagt attagtgatg
     2941 aagagtttaa acaagctatt aaagaagttc ttagtatagt gaaaaactat aatcatgaaa
     3001 ttacaattat agaatgtgat aatgaaatta gacgtgtata taaagtgaga actgaaaaag
     3061 atataaaaga tagaattagg ataggtggag gtactagatt ttctccagtc tttgagtatg
     3121 ctaataataa aaagattaat ttgttagtct actttactga tggtaaaggt gaaaataaac
     3181 tacaagtaat acctagaggg tataaaattt tatgggttat ttctggaaga ggagataaaa
     3241 tttcattaaa agaaccttat ggagcagtta aaaaacttac taaagttaaa acaaatgatg
     3301 aaatattaga aataaatgat gtaagaaatg atgggtactc aatgaatagc caagcaccaa
     3361 ttttataggt attcacagga gataagctgt attaatttat tttgatatgc ttgttataaa
     3421 aatctaaaaa atacattaat caatggttaa tgtatttttt aattttaata atataagttt
     3481 tacttacatt taagtcttaa aatgtgttaa aatataatat atagcagata gttttggcaa
     3541 ggtggattta gttgtcaaaa ctattttttt tattaaggat ggtgatgttt ttgtttaaga
     3601 ttgatgatta tataatgtat ggtatgacag gagtctgcaa agtcatggat ataacaaatg
     3661 aacgatttac aaatggaata aaaaaagagt attatgtatt gagtcctatt tattctaata
     3721 atacagtaat aaaaattcct gtagataatg aaaaagtacc aatgagaaag ttgctttcta
     3781 aaattaatgt gctttcatta ataaatgata ttcctaatat ggatacatca tggatagata
     3841 atgaaaaatt gagaagtgag caatttaaaa aaatactcag aggtggtaaa tgtgaagagt
     3901 taataaaatt agtaagaagt atagataata acagagagta tgtaaaatct attggtaaaa
     3961 aaacacatca agcagatgat aatataatga aagaagctga aaggttatta aatgaagaat
     4021 ttgcaactat tttagatatt tctcctaatg aagttagttc atatatttca agtcatatac
     4081 ctcaataaag tgttttagta tcctttattt aatctatttt aagtattatt tatgtatgct
     4141 ggttttggta aaataaaata tataattaaa ttatataaaa aataataatg attgtattga
     4201 agaattacag aatagtgcgg agagcctact cctttaggtg tgagatggat atcacttaaa
     4261 gatttaaatt gttattgtag atgtttttaa tataaaacaa gattaataag tgtattgtaa
     4321 gctttaagtg aaattgatac tataatgagt tacttagatt ttaaaatata taatattata
     4381 aaaaatcctt ttaggttttg aatgaataag atggatacta tgttgaatag aatccatctt
     4441 atttaagttt tatttatttt gtttatttgt aagcattatt tttgtacatg aaaacacagt
     4501 gtaaaaatat gcttagaatt atgaagtttt attaaaaaga tattcgacaa aataagacta
     4561 aaaatattct taaacttata actaagataa atattgtata tttatagtgt agcctgaaaa
     4621 attgtggatg tacgaaattt atttagtatt ttattgaaac aaagtagaag aagtactata
     4681 gtttatcttt gaatagattg ttatttttta gtgaaaaagt tattatgtta atacataaat
     4741 ataatagctt ataaatagtt taagcatgtt aaaaaagtag ttattagaga tatatagtta
     4801 cattgcttat aagatactta aaatgatagc attcacttat atttagaaaa ttaaga
//
LOCUS       DBGJFF010000206         4731 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.211, whole genome shotgun sequence.
ACCESSION   DBGJFF010000206 DBGJFF010000000
VERSION     DBGJFF010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4731
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.211"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            232..1383
                     /locus_tag="ACOIAF_003678"
     CDS             232..1383
                     /locus_tag="ACOIAF_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HGZ2670656.1"
                     /translation="MEIYLDNSATTKPYQEVIDKMVYALNTEYGNPSSVHRKGVEVEK
                     AIKEVRQDIAKSLGAKEKEIYFTSGGTECNNTIIRGITSLNKKRKNHIISTNIEHPSV
                     LNTLKDLEEDGFEVTYLEVGKDGKINIEDLKNAIKSTTCLVSMMHVNNEIGTIQPIGE
                     VGKYLKGLKEKIYFHVDAIQSYGKINFRPSKYNIDFMSVSAHKFHGPKGIGFMYIKEN
                     NRLKPMLTGGGQEIGIRSGTENVPGIYGLGEAVRILNKDLDAVISKVNNLKNILKNEI
                     IDNIEDIKINSPEDGVCHILNVSFRGTKGEVLLHYLEQKGIYVSTGSACSSKKKGSYV
                     LNAIGLTNEEINGTIRFSLSDMNTEEEMLEAVKVLKESICDLRSIMKRK"
     gene            1429..2604
                     /gene="thiI"
                     /locus_tag="ACOIAF_003679"
     CDS             1429..2604
                     /gene="thiI"
                     /locus_tag="ACOIAF_003679"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HGZ2670657.1"
                     /translation="MVYNILIVKYGEIGVKGKNRYIFENRLIRNIRNMLKPIGRFNVY
                     KEYGRIYVDLEDYDYEEVIEEVRKVFGIVGVCPGVRAKKDYDTLKEIALKMLEEKIEA
                     GYKTFKVESRRGDKSFGLTSQEMSMDIGGYLLSKVGDRINVDVRNPEVKIKCEYREFH
                     TMVYSDTIPGYGGLPLGTNGRAMSLLSGGIDSPVATWMVAKRGMEVEAVHFHSYPFTS
                     ERSQEKVKDLAKILAKYCGRVRLHKVNILEIQKAIGENCNEEEATILSRRFMMRIAQR
                     LSEKRHCDALITGESIGQVASQTIQGLTCTNAVVDLPVFRPLIAMDKSDIVDIAKKIG
                     TFETSIVPEEDCCSVFSPRKPVTKPRLEKIEKSETALDIEKLVQDAIDKIEVEDIEF"
     gene            2740..3543
                     /locus_tag="ACOIAF_003680"
     CDS             2740..3543
                     /locus_tag="ACOIAF_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HGZ2670658.1"
                     /translation="MNKKGFTLIELLVVISIIGILVIVAVPALFRNIEKSKAVTCLSN
                     RENIKTQIVIAMAEESSKDKNEVIKEVLENKDGKYFETEPKCKSGGIYSATFDDGYDG
                     ITGRESIAKVYVTCTKHPDGVEMARDVHQSMKDLIASFAQDPSIIPGASKSNDDFRKY
                     LLDNKYKNGWPTIPDEFKAKYGLSKDTLYIQPYAYNPTKSDATVVVFANNKTGGNWYT
                     SLVYDYDEGRWYKGKNGISVAGRSWDVDTDSVKSVKTEIHSKEGWGPLN"
     gene            3786..4427
                     /locus_tag="ACOIAF_003681"
     CDS             3786..4427
                     /locus_tag="ACOIAF_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HGZ2670659.1"
                     /translation="MNNNFQDNQTINLIQSRRSIRKFTTEQVSDEQVNTLLHCAFAAP
                     SGCNKQPWHITVVQDQKLLKEISDDTLSRIHEVSNVEINKNFKLFYGAPTVLFISYDE
                     SSSWAPYDIGILTGNITTAAQALGLGSCIIGMVRGLFTPVEQGDIEGLVSVLDKEDVK
                     ESESIKMKFDTNKKYRELLDIPEGYSVPFGIAVGIPDGNLPNAREVVYKVSRV"
BASE COUNT         1881 a          451 c          918 g         1481 t
ORIGIN      
        1 tatattattt tatcatatat tgtattttat aatatatttt tgtagggtat agtataagta
       61 tattatattt tactataaaa atttgtaata aagtgaaaaa actagctttt catgaaataa
      121 tattttataa tttaagattg tctttttaat aaagattaac aaaaaaaata ctataattag
      181 tggtataata gttagagttg aagattttaa ataaaggtag gtgtaattaa gttggaaata
      241 tacttagaca atagcgcaac tacaaaacct tatcaagaag ttatagataa aatggtttat
      301 gcacttaata cggaatatgg aaatccttct tctgtccata gaaaaggtgt agaagttgaa
      361 aaagctataa aagaagtaag acaagatata gctaaatctc taggagcgaa agaaaaggag
      421 atttatttta catcaggtgg tacagagtgt aataatacta taataagagg tattactagt
      481 ttaaataaaa aaagaaaaaa tcacataata tctactaaca tagaacatcc ttctgtttta
      541 aatacattaa aagatttaga agaagatggt tttgaagtaa cttatttaga ggtcggaaaa
      601 gatgggaaaa taaatataga ggatttaaaa aatgcgataa agtcaactac atgcttagta
      661 agcatgatgc atgttaacaa tgaaatagga actatacagc caataggtga agttggaaag
      721 tatttgaagg gtttaaaaga aaaaatttat ttccatgttg atgctattca gtcatatgga
      781 aaaattaact ttagaccatc taaatacaat atagatttta tgagtgtaag tgcacataaa
      841 tttcatggtc caaagggaat tggatttatg tatataaaag aaaataatcg ccttaagcct
      901 atgttaacag gtggtggtca ggaaattgga attaggtcgg gaacagaaaa tgtacctgga
      961 atatatggtc ttggagaagc tgttagaata ctaaacaaag atttagatgc tgttatttcg
     1021 aaagtaaata acttaaaaaa tatattaaaa aatgaaataa ttgataatat agaagatata
     1081 aaaatcaatt ctccagaaga tggagtatgt catatattaa atgtatcatt tagagggaca
     1141 aaaggagaag ttttacttca ttacttagag caaaaaggta tttatgtatc aactggctct
     1201 gcatgttctt ctaaaaagaa aggtagttac gtattaaatg ctataggtct tacaaatgaa
     1261 gaaataaatg gaacaataag atttagttta tctgatatga atacagaaga agaaatgtta
     1321 gaagctgtga aagttttgaa agaatcaatt tgtgacttaa ggtcaataat gaaaagaaaa
     1381 taaagaatac tgtatagata gtgataataa attagtttaa gaggtgaaat ggtgtataat
     1441 atattaatag taaagtatgg agaaattgga gtaaaaggaa agaatagata tatatttgag
     1501 aataggctta taagaaatat aagaaatatg ttaaaaccaa ttggaagatt taatgtatat
     1561 aaagagtatg gaagaatata tgttgattta gaagactatg attatgaaga agtaattgaa
     1621 gaagttagaa aagtatttgg gatagtgggt gtgtgccctg gagtaagagc taaaaaagat
     1681 tatgatacat taaaagaaat agctttaaaa atgttagaag aaaaaataga ggctgggtat
     1741 aaaactttta aggtagagtc aagaagagga gataagtctt tcgggttgac ttctcaagaa
     1801 atgagtatgg atataggtgg atacttatta tcaaaggtag gagataggat taatgttgat
     1861 gtaagaaacc cagaagttaa aataaagtgc gaatacagag aatttcatac tatggtctat
     1921 agtgatacta tacctggata tggtggattg ccacttggaa cgaatggtag agctatgtct
     1981 cttttatcag gaggtataga tagcccagtg gctacatgga tggttgcaaa aagaggtatg
     2041 gaagtagagg cagttcactt tcatagttat ccatttacaa gtgaaaggtc acaggaaaaa
     2101 gttaaggatt tagctaaaat actggcaaaa tactgtggta gagtaaggct tcataaagtt
     2161 aatatcttag aaatacaaaa agcaattggt gagaattgta atgaagaaga agctactatt
     2221 ctttcaagaa gatttatgat gaggatagcg cagaggcttt ctgaaaaaag acattgtgat
     2281 gctttaatta caggtgaaag tattgggcag gttgcatctc aaactataca agggcttact
     2341 tgtactaatg ccgttgtaga tttaccagtg tttagaccac ttatagcaat ggataaatca
     2401 gatatagttg atatagccaa aaaaataggg acttttgaaa catctatagt gcctgaagaa
     2461 gattgttgta gtgtattttc tcctagaaag cccgtcacta aacctagatt agagaaaata
     2521 gaaaaatctg aaacagcact tgacattgaa aaattagtac aggatgctat tgacaaaata
     2581 gaggtagagg atattgagtt ttagcatacg agttatttaa ttattaaatt gaatattgag
     2641 tatattagta gttctgttga aaggatttat gtagacaatt tattaatatg tagtatttaa
     2701 aaataagtac tataatttta tttaatggga atgattatta tgaataaaaa gggttttaca
     2761 ttaattgaat tgttggtagt tatatctata ataggcattt tagttatagt agctgttcca
     2821 gcgttattta gaaatataga aaaaagtaaa gcagttacat gtctttctaa tagagaaaat
     2881 ataaagactc aaattgttat tgcaatggct gaggaatcaa gtaaagacaa gaatgaagtc
     2941 ataaaagagg tattagaaaa caaagatggt aagtactttg aaacagaacc aaagtgtaag
     3001 tcaggtggaa tatattcagc aacgtttgat gatggttatg atggaatcac tggaagagaa
     3061 agcattgcaa aagtgtatgt tacttgtaca aaacatccag acggtgttga aatggctagg
     3121 gatgtacatc aaagtatgaa agatttgatt gcatcatttg cacaagaccc ttctataata
     3181 ccaggagctt caaagagcaa tgatgatttt agaaaatatt tattagacaa taaatataaa
     3241 aatgggtggc ctacaattcc agatgaattt aaggcaaaat atggattaag taaggataca
     3301 ctatatatac aaccatatgc atataatcct actaaatctg atgctactgt agttgtattt
     3361 gcaaataata agactggagg taattggtat acttccctag tttacgatta tgatgaaggt
     3421 agatggtata aaggtaaaaa tggtatttct gttgcaggta ggtcatggga tgttgacaca
     3481 gatagtgtta agtctgtaaa aacagagatt cattctaaag agggatgggg tcctttaaat
     3541 taatatatca tgttttatta atctggtatt gattaaacta tagaaaagag cagtttctat
     3601 agatgacgaa taccagattt tcatttatat atataattga gatatattaa aagtgtttat
     3661 atttttaagt cttcacatat aagctaaaaa taattataat aatattgtaa agaaatttaa
     3721 ctataagatt aattttctga agatttagta tttgtactaa gaaatacata aaaaggggaa
     3781 tagatatgaa taataatttt caagacaacc aaacaataaa tttaatacaa agtcgtagaa
     3841 gtatacgaaa atttacgaca gaacaagtta gtgatgagca agtaaacaca ttattacact
     3901 gtgcatttgc agcaccttca ggatgtaata aacagccttg gcacataact gttgtgcaag
     3961 accagaaact tttaaaagag attagtgatg atactttatc taggattcat gaggtgagta
     4021 atgttgagat aaataaaaac tttaagttat tttatggtgc tccaaccgtt ttatttattt
     4081 cttatgatga aagtagttca tgggctccat atgatatagg aatacttaca gggaatataa
     4141 caacagctgc acaagcacta gggcttggaa gttgtataat aggaatggtt agaggtttat
     4201 ttacacctgt tgaacaaggg gatattgaag gtcttgtgag tgtattggat aaagaagatg
     4261 taaaagaatc agagtcaata aagatgaagt ttgatacaaa taaaaaatac agagaactac
     4321 ttgatatacc agaagggtat tctgtaccat ttggtattgc tgtaggtatt ccagatggaa
     4381 atttaccaaa tgctagagag gtagtttata aagtttccag agtttaggat aaagtattgc
     4441 aagaaccaat cagttacttg aagaattaaa ccagtaatat acaaataaat tgatggaaga
     4501 ggaagctatt taaaaagtag tttagatttt tcgttgtaaa ataattaaat aaagaataag
     4561 gtgataagta ttttatttat tatcttattc ttttttattt ttgttttgta gaatgataaa
     4621 aataaggatt aagtgtaata aaatagaaaa caagtatgtt aattttaagt atatgcttta
     4681 aaatttatcc aagttaaaaa tttaagatat cttttcagta taatggaaaa a
//
LOCUS       DBGJFF010000207         4658 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.212, whole genome shotgun sequence.
ACCESSION   DBGJFF010000207 DBGJFF010000000
VERSION     DBGJFF010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4658
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.212"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(<1..882)
                     /locus_tag="ACOIAF_003682"
     CDS             complement(<1..882)
                     /locus_tag="ACOIAF_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HGZ2670660.1"
                     /translation="MNLNKTEFLEILKDYLKKDFSESEVNDILRDYEEYFVDGLIEGK
                     SDMEIIASLGSPKSIAKELILQTKEKNIEEEKIINTDVLNDKFSKFKSDMKKGYSILK
                     DEAKEKYSKSKENIDGKIMNTDILNGKFSRFKSNVKKDYSILKDEAKEKYNKSKENIN
                     DKLTPSIEEGVGGLSRQVIKGLLVVLSLFLIVPAFAFVCAIVSATIALVCSLIAFVIL
                     IPFVIKFIAMVPDVMMFCIFATIAFVGLEILAWQILLFAIKLGKQLLNNYIHWIKRKN
                     IYIKANEKMEKRKKATLN"
     gene            complement(869..1201)
                     /locus_tag="ACOIAF_003683"
     CDS             complement(869..1201)
                     /locus_tag="ACOIAF_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HGZ2670661.1"
                     /translation="MNTQFRKGVLEICVLALISKKDMYGYEIVHNISKVIDVNEGTIY
                     PILRRLTKDLYFETYILESNEGPARKYYRITSLGKENLSNLMEEWREFVKAVEILISE
                     DEGGNELE"
     gene            1400..2521
                     /locus_tag="ACOIAF_003684"
     CDS             1400..2521
                     /locus_tag="ACOIAF_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HGZ2670662.1"
                     /translation="MKDDKTILILTAQFGAGHISAAKAVKECIIEKYSNYNVVIQNFI
                     NASIPMMNKPMVKLYENNTKYTPGLYNYYYYFKKSFDSRHDLSHKLYTPKLSEYIADI
                     NPDLIISTFPLAAACVNNFKIKNPDINIPTLTVITDVVDSMEWVFENTDLYFVPSPEI
                     KNRFFQKGINPDSIKVTGVPVDKRFQIESKELCCDKYRLLLLGGGRGLFDIDEDFMHW
                     IDEFIEEHSNSIEITIVTGKNKKLYDNLTHKKPLKNIKVLGFVNDMYNLIRECDLMLT
                     KPGGATIFEAIQSQTPVLVKMPKVGQEIENAKFIIDKGLGMIYSDDLDLKNIFYKLVS
                     NEFDSIINFMKKNLKEFKTVIHPEKIADYISELIDNHYS"
     gene            complement(2649..4547)
                     /locus_tag="ACOIAF_003685"
     CDS             complement(2649..4547)
                     /locus_tag="ACOIAF_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HGZ2670663.1"
                     /translation="MDSLLYVIEKDKHTNEELREILKSNKNIRFVSLMGVDLGGNATD
                     EKIPVELFLEDIDKFLESAIQTDGSSVELYNIATLNNAKVDLMPDKSCHWYVDYNMEY
                     IDEEVGLPVGTLKIPAFLIHDNKKVCSRGVLQKADKYFKKSMYEIFREYPHVINNIGI
                     DSVDDIEEIMLTAATELEFWVNTPEDKADLEKLYVSQSLKEQYWKRTHGIIRTCLEKS
                     LIILQKLGVNPEMAHKEVGGIQSSISIDGRTNHAMEQLEVSWKFSTPLQAADNELLVR
                     DVIEDVFTSHGLEVTFKAKPIHGVAGSGGHTHVGVSAKLKDGSIKNLFAPKDLKEDYL
                     SELGYGALMGLLYNYEVLNPIVTASNDGFNRLVPGFEAPVCIVTSLGHSYEIPSRNRS
                     VLVGLIRDMKNPKTVRFELRSPSPLSNTYLVIAGCYQTMLDGIKAAAKSGLSTKELEK
                     ELSKNVGEESFYLEKDRAYRDENDVFEHYSLEERNARFGIPPATVYENMKNLEIYASK
                     LKSLKQGDVFTDSIIESFKIGAIDKWQKKLKTRIIEAGIQKIRSIVKIHTKENMDALD
                     EVVWNSISDLKFNIMKDTLTRESLFTRVREAIENKDYQTASDLQIELKRSMEEIQQLY
                     MQYKKNIY"
BASE COUNT         1510 a          792 c          532 g         1824 t
ORIGIN      
        1 atttaaagta gctttttttc ttttttccat cttttcattt gcctttatat aaatattttt
       61 tctctttatc caatgtatat aattatttag taattgttta cctagtttta tggcaaataa
      121 taaaatttgc catgcaagta tctcaagccc tacaaatgct atggttgcga aaatacaaaa
      181 catcataaca tctggaacca ttgctataaa tttaatcaca aatggaatta atataacaaa
      241 agcaattaat gagcatacta atgcaatagt agcagataca atagcacata caaacgcaaa
      301 tgctggaaca attagaaata aagataacac tactaataac ccctttataa cttgtctgga
      361 taatcctcct acgccttctt ctatacttgg tgtaagttta tcatttatat tttcttttga
      421 tttattgtac ttttccttag cttcatcttt caagatagag taatcctttt ttacatttga
      481 tttaaatcta ctaaatttac catttaatat gtctgtattc attatttttc catctatatt
      541 ttcttttgat ttactatact tttcttttgc ttcatctttt aagatagagt aacctttttt
      601 catatctgat ttaaatttac taaacttatc attcaaaaca tctgtattta ttattttttc
      661 ctcttcaata tttttttctt tagtttgtaa gataagttct tttgctatag attttggtga
      721 gccaagtgat gctattatct ccatatcact ctttccctct ataagaccat ctacgaaata
      781 ctcttcataa tctctcaata tatcattaac ttcgctctca gaaaaatctt tttttagata
      841 atcctttaaa atttcaagaa actcagtttt attcaagttc attacctccc tcatcttcac
      901 ttattaaaat ctcaacagcc tttacaaatt ctctccactc ttccatcaga ttggataaat
      961 tctcttttcc tagtgaagtt attctatagt attttctagc aggaccttca ttagattcta
     1021 gtatatatgt ttcgaaatac aaatcttttg taagtcttct tagaatagga tatattgttc
     1081 cttcatttac atctattact ttagagatgt tatgtactat ctcatatcca tacatatctt
     1141 tcttagatat gagagcaagt acacaaattt ctaaaacacc ctttctaaat tgtgtgttca
     1201 tatactccat cctttcttta aatacacagt attgtgtttt acaaggtact atgttaaaac
     1261 ttcttatatc ctcataataa cacagtacta tgttctgcac aatagttatt tttaaatatt
     1321 cttgttgatt aaatccttct ataaatgtat aatactctaa tcagatatat tttatgaaag
     1381 tggtgataaa ttggctaaaa tgaaagatga taaaacaata ttaatcctaa ctgctcaatt
     1441 tggtgctgga catataagtg ctgcaaaagc agtcaaagaa tgtattattg aaaaatatag
     1501 taactataat gttgttatac aaaactttat aaatgcaagt attcccatga tgaataagcc
     1561 tatggtaaaa ctttatgaaa acaatacaaa atatactcct ggattatata attactatta
     1621 ttactttaag aaatcgtttg attcaagaca tgatttatct cataaattat atacacctaa
     1681 actttctgag tatattgctg atataaatcc agatttgatt atctctacat ttccactagc
     1741 tgctgcttgt gtaaataact ttaagataaa aaatcctgat ataaatatac ctacacttac
     1801 agttataaca gatgttgtag atagtatgga atgggttttt gaaaatacag atttgtattt
     1861 tgttccttct cctgaaataa aaaatagatt ttttcagaaa ggaataaatc ctgactccat
     1921 aaaggttact ggagttcctg ttgataagag atttcaaatt gaaagtaaag aactatgttg
     1981 tgataaatat agactattac ttctaggtgg aggaagaggt ctatttgata tagatgagga
     2041 ttttatgcat tggatagatg aatttattga agaacatagt aattctatag aaattacaat
     2101 agttactggt aaaaacaaaa aattatatga caatctaact cataaaaaac ctctaaaaaa
     2161 tattaaagta cttggatttg taaacgacat gtataatcta ataagagaat gtgacctaat
     2221 gctaactaag cctggaggag caactatatt tgaagctatt caatcacaaa ctccagtact
     2281 cgtaaaaatg ccaaaagttg gacaagaaat tgaaaacgct aaatttataa tagataaggg
     2341 tcttggcatg atttatagtg atgacctaga ccttaaaaac atattttata aattagtatc
     2401 aaatgaattt gattctataa taaactttat gaaaaaaaat ttaaaagaat ttaaaacagt
     2461 cattcatcca gagaaaattg ctgattatat atcagaatta atagataatc attacagtta
     2521 aattgtacta ttaaaaaatt tcaaacataa aattaaaggc aatcaagtat gcttttacac
     2581 ttgagaattt actcatagtg tttaataata cttgattgcc tttatcatat caaaatcaaa
     2641 ttgttatatt agtatatatt tttcttgtac tgcatataca gttgttgtat ttcttccata
     2701 cttctcttta attctatttg taaatctgaa gcagtctggt aatccttatt ttctatagct
     2761 tctctcactc tagtgaatag tgattctcta gttaaagtat ccttcataat attgaacttt
     2821 aaatccgata ttgagttcca aactacctca tcaagcgcat ccatattttc ttttgtgtgt
     2881 atcttgacaa tacttcttat tttttgaatc ccagcctcta ttattctagt ttttaatttc
     2941 ttttgccact tatctatagc tccaatctta aatgattcta ttatactatc agtaaataca
     3001 tcaccttgtt ttaaagattt taattttgaa gcatatattt ctaagttttt catattttca
     3061 taaactgtag ctggtggtat tccaaatctt gcatttcttt cttccaaact ataatgttca
     3121 aatacatcat tttcatctct atatgctcta tctttttcta aatagaaact ttcttcacct
     3181 acatttttag ataactcttt ttcaagttct tttgtactaa gaccactctt agctgctgct
     3241 tttataccat ccaacattgt ttgataacat cctgctatta ctaaatacgt gttagataga
     3301 gggcttggtg accttaattc aaatcttaca gtctttggat ttttcatatc tcttattaat
     3361 ccaacaagca ctgacctatt tcttgatgga atctcataac tatgccctaa agatgtaact
     3421 atgcatacag gagcttcaaa tccaggaact aatctattaa atccatcatt agaagctgtt
     3481 acaattgggt ttaatacttc ataattgtaa agaagtccca taagagcccc atatcctagt
     3541 tcacttagat aatcttcttt taaatctttt ggagcaaata gattttttat acttccatcc
     3601 tttaacttcg cacttactcc tacatgtgta tgtccaccac ttcctgctac tccatgaatt
     3661 ggttttgctt taaatgtaac ttctagtcca tgtgatgtaa atacatcctc tataacatct
     3721 cttactaata attcattgtc tgctgcttgt aatggtgtag aaaacttcca agaaacctct
     3781 aattgctcca tagcatggtt tgttcttcca tctatactaa tagaactttg tattcctccg
     3841 acctctttgt gagccatttc agggtttaca cctagctttt gaagtataat taaacttttc
     3901 tctaaacaag ttcttataat accatgagtt cttttccagt attgctcttt taggctttgt
     3961 gatacatata atttctctaa gtcagcttta tcctcaggag tattaaccca gaactcaagt
     4021 tctgtagctg ctgttaacat tatttcctct atatcatcta ctgaatctat tcctatgtta
     4081 tttattacat gtggatattc tctaaatatt tcatacattg actttttaaa gtacttatct
     4141 gctttttgaa gtactcctct agaacatact tttttattat catgtattaa aaatgcaggt
     4201 atctttaatg ttccaacagg tagacctact tcttcgtcta tatattccat attgtaatct
     4261 acataccaat gacaactttt atctggcatt aaatctactt tcgcattatt taatgtagct
     4321 atattataaa gctcaacact tgaaccatca gtctgtatag ctgattccaa aaatttatct
     4381 atatcctcca aaaataattc tacaggaatc ttttcatctg ttgcatttcc tcctaaatca
     4441 acccccatta aggaaacaaa tcttatattt ttattgcttt ttaggatttc tcttaattct
     4501 tcattagtgt gtttatcttt ctctattacg tataatagac tatccatttt tccactctcc
     4561 cataattttt atgtatattt tattttgtta aatacaagat aaatttcatt ttccttacat
     4621 ttatgaattt ttcttgcaaa ttattcgtgt tttattcg
//
LOCUS       DBGJFF010000208         4542 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.213, whole genome shotgun sequence.
ACCESSION   DBGJFF010000208 DBGJFF010000000
VERSION     DBGJFF010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4542
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.213"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            319..1794
                     /locus_tag="ACOIAF_003686"
     CDS             319..1794
                     /locus_tag="ACOIAF_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HGZ2670664.1"
                     /translation="MDSEFANVVELSYECAPLLESLKEYSEKDIACFDVPGHVKNRGV
                     AILNKYLGESLMKMDINSSPNMDNVSAPNGIIKNAQDLLAQAYMADEAFFITNGTTQA
                     IHAMILSVIKPGEKVLLPRNIHKSVINALILCGGIPIFIQPEFDEKLGISLNITLEKV
                     RTEIEKDCNIKALFFLNPTYYGVCADLESIIELCHKNNVLVLVDEAHGAHFQFHLDLP
                     PSAISLGADMVAVSIHKTGGALTQSSALLLNRDNVSFEKVLQSINMLQSTSASYLLMA
                     SIDGARVNLVENGEKQLSKALNLSRYAKSKLNKIDGIKVLSTEILKQKGVKFIDETKL
                     CINVKELNLTGFEVYDLLYKNFSIQVELGDSYNILALVSIGTNKSDIDRLVKALSIIA
                     KIYRKESTLNEFNMVQINPIIKLNPREAFYAPKESVEINSCIDRICGESIMAYPPGIP
                     IIAPGELITEEIMEYIIFLKNSNAYLTDVQDKNLDRILVIK"
     gene            1881..2294
                     /gene="speD"
                     /locus_tag="ACOIAF_003687"
     CDS             1881..2294
                     /gene="speD"
                     /locus_tag="ACOIAF_003687"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HGZ2670665.1"
                     /translation="MKFETLGRHILVEYYNCDENVLKDPRLIENFMNDSALNAKATIV
                     DSVFHHFNPWGVSGAVIIAESHLTIHTWPEYGYAAADFFTCGDIDPWKSFELLEQLLK
                     SERSESTEIPRGLTTKIKKYSKKDLGKITHKPEAV"
     gene            2324..3175
                     /gene="speE"
                     /locus_tag="ACOIAF_003688"
     CDS             2324..3175
                     /gene="speE"
                     /locus_tag="ACOIAF_003688"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HGZ2670666.1"
                     /translation="MELWYTEEWTENVRFSIKVNKHLFEGKSQFQRIDVFDSDEFGKF
                     LTIDGLMMVTYKDEFIYHEMITHVPMATNLNIKKVLVIGGGDGGTVRELSRYPQIEKI
                     DMVEIDKMVVDVSKEYMDICSCKLDDKRVSLYFEDGVNFVKCAHDKSYDLIIVDSTDP
                     IGPGEGLFSTDFYKDCYRILTDDGILVNQSESPYFDFNAKEMKRANKKLKQIFPISEV
                     YQAHIPTYPSGHWLFGFASKKLNPVKNQDRNGWEKLSLKTKYYNSDIHLGSFMLPQYV
                     KEMLDEE"
     gene            3165..4043
                     /gene="speB"
                     /locus_tag="ACOIAF_003689"
     CDS             3165..4043
                     /gene="speB"
                     /locus_tag="ACOIAF_003689"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HGZ2670667.1"
                     /translation="MKNNFYHMNTFMSMDKNYEESNLIVFGVGFDGTTSNRPGARFAS
                     SSMRKEFYGLETYSPFLDLDLEDYNICDYGDLEISVGSTEQVLKEIYQETYKIVRDSK
                     VPFMIGGEHLVTLPAFKAVHEKYNDIYVIHFDAHTDLREEYNNSKNSHATVIKRIWDI
                     VGDNKIFQFGIRSGTKEEFKFATEEKHTYMEIGGIDTFENIVNILNGKNIYLTIDLDV
                     LDASVFPGTGTPEPGGVNYREFQEIFKIIKNSNINIVGCDIVELSPDYDTTGVSTVIA
                     CKILRELCLIISDKIK"
     gene            complement(4223..4423)
                     /locus_tag="ACOIAF_003690"
     CDS             complement(4223..4423)
                     /locus_tag="ACOIAF_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HGZ2670668.1"
                     /translation="MKNGIVKWFNNEKGFGFISVEGEDDVFVHFSAIQNDGYKTLEEG
                     EKVSFDITQGNRGPQAENVNRI"
BASE COUNT         1826 a          503 c          760 g         1453 t
ORIGIN      
        1 tataaattaa aaatagtaaa ctcaaaagct tactaaaact caaaaaagta aaaaaaccaa
       61 aataataaaa aataagaaag tttataaata ctaagtttta aaaaaattaa tataaacaac
      121 aaaaaattaa aaagtttata aatattgata tagctaatgt atatgttttt gagtaacttc
      181 aaaaaaacac ttgtaaaaat gctgggatgt taatataata aatatcatga gtttaataaa
      241 agtaaaaata aaaaatagaa ataataaata ataaatatta aaaatatttg aatatagaga
      301 ggagatgaat ttgaaatcat ggatagcgag tttgcaaatg tagttgaatt aagctatgag
      361 tgtgctccat tattagagtc gttaaaggaa tactcagaga aagatatagc ttgttttgat
      421 gtgcctggac atgtaaaaaa tagaggtgta gctatattaa ataaatatct aggtgaaagt
      481 ctcatgaaaa tggatataaa ttcatcacca aatatggata atgtatctgc gccaaatggg
      541 attataaaaa atgcacaaga tttgctagca caagcatata tggctgatga ggcattcttt
      601 ataacgaatg gcacaactca agcaatacat gcaatgattt tgagtgttat aaaaccaggt
      661 gaaaaagtat tacttccaag aaatatacat aaatcagtaa taaatgcgtt aatactttgt
      721 ggaggaatac caatatttat acaaccagaa tttgatgaaa aactaggtat aagtttaaat
      781 attactcttg aaaaagttag aacagagata gaaaaagatt gtaatataaa agcattattt
      841 ttccttaatc caacttacta tggagtttgt gcagaccttg agagtattat agaactatgc
      901 cataaaaata atgttttagt gttagttgat gaagctcatg gtgcacattt tcaatttcat
      961 ttagatttac ctccttctgc aataagtttg ggagctgata tggtagctgt aagtatacat
     1021 aaaactggag gagcattgac acaatcttca gcactcttat tgaatagaga taatgtaagt
     1081 tttgaaaagg ttcttcaatc aatcaatatg ctacaatcaa catcagcatc ttatttgctt
     1141 atggcaagta tagatggtgc tagagttaat ctagttgaga atggtgaaaa gcaattgtca
     1201 aaagcattaa atctatctag gtatgcaaaa tctaaattaa acaaaattga tggaataaaa
     1261 gttttatcta cagaaatatt gaaacaaaaa ggtgttaaat ttatagatga aacaaaattg
     1321 tgtataaatg taaaagaatt aaatttgact ggatttgaag tatacgattt attatataaa
     1381 aatttttcaa ttcaagtaga attgggagat tcatataata tactggcact tgtgtccatt
     1441 ggaacaaata aatcagatat agatagatta gtaaaagcac tttctataat tgcaaagatt
     1501 tatagaaaag aatcaacatt aaatgaattt aatatggttc aaataaatcc aattattaaa
     1561 cttaatccaa gagaagcttt ttatgcacca aaggaaagtg tagaaatcaa ttcatgtata
     1621 gatagaattt gtggagaatc aataatggca tatccaccag gtataccgat tattgctcca
     1681 ggagaattga taacagaaga aattatggag tatattattt tcttaaaaaa tagcaatgca
     1741 tatcttacag atgtacaaga taaaaactta gatagaatat tagtaataaa ataaattact
     1801 ttattttgca agggcaagta tttgttaagt agagtttatc aaatagaact tattaaaagt
     1861 ataaaggagt tggggttgaa atgaaatttg aaacactagg aagacatata ctagtagaat
     1921 actataattg tgatgaaaac gtacttaaag acccacgtct tatagaaaat tttatgaatg
     1981 attcagcatt aaatgcaaaa gcgacaattg tggattctgt attccaccat tttaatcctt
     2041 ggggggtttc aggagctgtt ataattgctg aatctcattt gacaatacat acatggcctg
     2101 aatatggcta tgcagcagca gattttttta cttgtggtga tatagaccca tggaaaagtt
     2161 ttgaattatt agagcaactt ttaaagtctg aaagaagcga gtctacagaa attccaagag
     2221 gcttaactac aaaaattaaa aaatattcaa aaaaagattt aggtaagatt actcataaac
     2281 cagaagcagt ataaaacata caagagaaag tgaggattat tatatggaac tttggtatac
     2341 agaagaatgg acagaaaatg taagattttc tataaaagta aataaacatc tatttgaagg
     2401 aaagtctcaa tttcaaagga tagatgtgtt tgatagtgat gaatttggaa aatttctaac
     2461 aatagatgga ttaatgatgg taacatataa agatgaattt atataccatg agatgataac
     2521 tcatgttcct atggctacaa atttaaacat aaagaaagta ttagtcatag gtggaggtga
     2581 tggaggtaca gtaagagaat taagccgtta tcctcaaata gaaaagattg acatggttga
     2641 aatagataaa atggttgtag atgtatctaa ggagtatatg gatatatgct catgtaaatt
     2701 agatgataaa agagtgagtt tatattttga agatggtgtt aattttgtta agtgtgcaca
     2761 tgataaatca tatgacctga taatagtaga ttcaactgac ccaataggac caggtgaagg
     2821 gcttttctca actgattttt ataaggattg ttatagaata ctgactgatg atggaatttt
     2881 agtaaatcaa agtgaaagtc cgtattttga ctttaatgct aaagagatga aaagagctaa
     2941 taaaaaatta aaacagatat ttccaatatc agaagtttat caagcacata taccaacata
     3001 tccatctggt cactggttgt ttggatttgc ttcaaaaaaa cttaatccag taaaaaatca
     3061 agatagaaat ggatgggaga aattaagttt aaaaactaaa tattataata gtgatataca
     3121 tttaggttcg tttatgttac ctcaatacgt gaaggagatg ctagatgaag aataatttct
     3181 atcatatgaa cacttttatg agtatggaca aaaattatga agaatctaat cttatagtat
     3241 ttggtgttgg atttgatgga actacttcta acagacctgg ggctagattt gcaagtagct
     3301 ctatgagaaa agaattttac ggtcttgaga catatagtcc ttttttagat ttggatttag
     3361 aggattataa tatatgtgat tatggagatt tagaaattag tgttggaagt acagaacaag
     3421 tcttaaaaga aatctatcaa gagacatata agattgttag agattcaaag gtacccttta
     3481 tgattggagg agaacattta gttacattac cagcctttaa agcagtacat gaaaagtata
     3541 atgatatata tgtaattcat tttgatgccc atactgattt gagggaagaa tataataata
     3601 gtaaaaattc tcatgcaaca gtaattaaaa gaatatggga tattgtaggt gataataaaa
     3661 tatttcaatt tggtataagg tctgggacaa aagaagaatt taaatttgct acagaagaaa
     3721 aacacacata catggaaata ggaggaatag atacgtttga aaatatagtt aatatactaa
     3781 atggaaagaa tatttatcta actatagatt tagatgtatt ggatgcatct gtgtttccag
     3841 gaacaggtac accagaacct ggtggtgtaa attatagaga gtttcaagag atttttaaga
     3901 ttataaaaaa ctctaatata aatatagttg gttgtgacat tgtagagtta agccctgatt
     3961 acgatacaac aggtgtatcg acagttatag cttgtaaaat cttaagagag ttatgcttaa
     4021 taatatctga taaaattaaa tagagtattt tatataaata gtatatatta taatataaga
     4081 agttatttta aaaaagttag tttaagtttg acgcttttct aaagtaactt cttatttttg
     4141 taattaaaaa taaatataat aaacaacaaa aaagagccct cgatagctcc ttttctaaaa
     4201 tatatatatt taaattaaat aattatattc tatttacgtt ttcagcttga ggtcctctat
     4261 taccttgagt tatatcaaag ctaacctttt cgccttcttc taaagttttg tatccatcat
     4321 tttgtatagc tgagaaatgt acgaaaacat catcttctcc ctctacagat atgaacccaa
     4381 atcctttttc attgttaaac cattttacta ttccgttttt cattataaga aaacctccgc
     4441 attattaaaa aatacaaact aaaaaaataa gtctgctcta ttaatataac ataaatttta
     4501 aataaaagca aataaaattt ttaaaaaaat taattacttt ta
//
LOCUS       DBGJFF010000209         4450 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.214, whole genome shotgun sequence.
ACCESSION   DBGJFF010000209 DBGJFF010000000
VERSION     DBGJFF010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4450
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.214"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(948..1358)
                     /locus_tag="ACOIAF_003691"
     CDS             complement(948..1358)
                     /locus_tag="ACOIAF_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670669.1"
                     /translation="MKIKKSLISAMMVSAMVLGGTGAVFASGNDSSNIALPKLESLIK
                     SGITDPTELEKYGVEHYTYEEYAKHIQELKDYAKDPNAVKDVSQKDLEETIKKMEQEL
                     EKIKTEGLKIMKPITIENEDGSQISIAYNYAAID"
     gene            complement(1574..2956)
                     /locus_tag="ACOIAF_003692"
     CDS             complement(1574..2956)
                     /locus_tag="ACOIAF_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HGZ2670670.1"
                     /translation="MFIKLLEMSFTSGVLLLIVIFIRACLFKKLPKKVFVLLWLIVLF
                     KLLIPVSLNSGFSIYSIIESTFDVEVGNTSTENYTLKKASDSTGKNNSDEFEIMSNIE
                     NIMMLEMLGILSFIVTIMFSILLCFRYFKIINLFKTSIHIKNHEFIKKWMCKNSRFRR
                     FIVCISEFTRTPLTSGILKPRIILPKQMNYANNQTLDFVLTHEMVHINSFDNLLKLFM
                     LVALVVHWFNPLVWVMFFLLNKDIEFSCDEKVMSILGEEYRADYATTLISLAEENNPF
                     SMTICSGFGKTKIEKRIVNIMKFKKSTYVTFLVSTVLVCGTGLVFATENKEINEVESF
                     GLTKNEQSDKIGFSISSKSTDLYSPKVESDILVSKAKSDGETHFSTAKVSVIEFREHE
                     ESSMSKVTNSENSEETYVNKSSNSKDVLYTDSGEVSIERSGYNDNRFVISRKGNLLAV
                     NKEQYRTWIE"
     gene            complement(2967..3326)
                     /locus_tag="ACOIAF_003693"
     CDS             complement(2967..3326)
                     /locus_tag="ACOIAF_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HGZ2670671.1"
                     /translation="MEIKLFDSELKVMEVLWERGESKAGELVKILKEETGWNRNTTYT
                     VIKKLVSKGAIERIEPNFVCKALISKEEVQKQETETLINKMFGGSSEKFLSAFINKEN
                     LSSDEITKLKQIVEKLK"
     gene            complement(3531..4310)
                     /locus_tag="ACOIAF_003694"
     CDS             complement(3531..4310)
                     /locus_tag="ACOIAF_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HGZ2670672.1"
                     /translation="MVSRQELKRVSKAQLSGNWGICAISMAVYIALIVLISIIGLPFE
                     PLTTIVVAVIAFCVSAGFTCMFLKITKDEKVKIGDIFVSGRIYLRSFGFYLFESIIVY
                     IYSFIITIIGAFLSFGVLLTSGAIEYLSSDNVGIGTIISTGITLVLIILVLMLPVYIL
                     LLYVSQATFLICEDKDNMGVFKSMGASLDMMKGNVLKLFVLNFSFIGWYILSIATLGI
                     GLLWLIPYVGVTNCNFYRQLVKENPDVEDILLDKEKSYTMY"
BASE COUNT         1539 a          757 c          397 g         1757 t
ORIGIN      
        1 tctaccatca tccatatatt tattttacga ttactctttc accctaaccc tttaactatc
       61 tattttttct tactattctt atccacatca tccatatatt tattttacga ttactctttc
      121 actctaaccc tttaactatc tattttttct gatattctta tccaccatca cccatatatt
      181 tattttacaa ttactctttt accctaaccc tttaactatc tatttttttc tggtattctt
      241 atctactatt atctgtatat ttattttatg attactcttt taacctaccc cctatacttt
      301 atagtaatat gtttttaacc tttttcatta ttcccaatat gctttataac tacaaatact
      361 ctcccccctt aaaaataata ataaaattaa tcaataaatt tataatcata ttattactct
      421 atgtattaag caattactat gccaataaac tttgattttt ttaaacaaat cttaaaaatc
      481 ctaaaatgct aattttcttt aacttttaat gaatctaata gataacattt aattttcgtt
      541 atatttttca gaatatttct attattattt ttaagaaaaa aaaataatgt taacatttct
      601 taacatacat taaaaaatgt taacattata aattgggttt aatactttta ctttgactcg
      661 atttactttt attctacttc ttactttatt tttaacttag actttatttt tttattactt
      721 atttgagtct tatttatttt attatcactc catatgttca attacatttg tctaaattct
      781 ttgatacttt tattacataa ttttaacaag cttaagtgtt atttgggtag tgcagttgat
      841 aattatttaa ataatcatca actgcatgtt tatatcatta cagtagctta atgtatttaa
      901 tcatacttac ttaggatttt gaaatatctt agagattata tttaacatta gtcaattgct
      961 gcataattgt aagcaattga aatttgagac ccatcttcat tttctatagt gataggcttc
     1021 ataattttta atccttcagt ttttatcttt tctaattctt gttccatttt cttaattgtt
     1081 tcctcaaggt ctttttgact tacatctttg actgcattag ggtctttagc atagtctttt
     1141 aattcttgaa tatgtttagc atactcttca taagtataat gctcaacacc atacttttcc
     1201 aattcagtag ggtcagttat tcctgatttt attagagatt caagtttagg taaagcaata
     1261 ttagatgaat catttccact tgcaaatact gctccagttc ctcctagtac cattgcagat
     1321 accatcattg cacttataag ggattttttt attttcataa ccaatcttcc tttcttagag
     1381 atttatttgg gtagtgtagt ttaaaatgac tattgcaata gttatcaacc acatccttag
     1441 actatcatag tagtcatgtt tatgtcaagc ctttttaaaa aacttttcaa taaaaaatac
     1501 tatctcaaaa caaaattaat tattttgaga tagtatttta attctaaatc ttttatatta
     1561 ctaagattta tggttactct atccatgttc tatattgttc tttattaact gctaacaaat
     1621 tcccttttct tgatatgaca aagcgattat cattatagcc acttcgctca attgaaactt
     1681 ctcctgaatc agtataaagt acatccttac tattactaga tttatttaca tacgtttctt
     1741 cactattctc agaattagtt actttgctca tagatgattc ttcatgctct ctaaattcaa
     1801 ttacagatac ttttgccgta ctaaaatgag tctctccatc acttttagct ttactaacaa
     1861 gaatatcaga ttctactttg ggagaatata aatcagttga tttactactt attgaaaatc
     1921 caattttatc actttgttca ttttttgtta aaccaaaaga ttcaacctca ttaatctctt
     1981 tattttctgt tgcaaaaaca agacctgtcc cacaaactaa tacagttgaa actaaaaaag
     2041 taacataagt tgatttttta aatttcataa tattaactat tctcttttct atttttgtct
     2101 ttccaaatcc actacatatt gtcattgaaa atggattatt ctcctctgct agacttatta
     2161 gtgtggttgc ataatcagcc ctatattctt caccaagtat actcataacc ttttcatcac
     2221 aactaaattc tatgtcttta tttagtaaaa aaaacatcac ccaaactaag ggattaaacc
     2281 aatggacaac aagagctaca agcataaata actttaacag attatcaaag ctattaatat
     2341 gaaccatttc atgagttaaa acaaagtcta aagtttgatt attagcataa ttcatttgct
     2401 ttggcaaaat aattcgtggt tttaaaatac cagatgtaag tggtgtccta gtaaattcag
     2461 atatgcaaac aataaatctt ctaaaacggc tattcttaca catccatttt ttaatgaact
     2521 catgattttt tatatgaatt gaagttttaa aaagattaat tattttaaaa tatctaaagc
     2581 ataaaagtat gctaaacata atagtaacta taaaagacaa aatgcccaac atttccaaca
     2641 tcataatatt ttcaatatta gacattattt caaattcgtc agaattgttt ttacctgtag
     2701 agtcactagc tttcttaaga gtataatttt ctgttgatgt attacccact tccacatcaa
     2761 aagtcgattc aattatacta taaatactaa aaccagaatt taaacttact ggtattagaa
     2821 gtttaaataa aacaatcaac cacaataata caaatacctt tttaggtaat tttttaaata
     2881 aacatgctct tataaaaata actattaaaa gtaatactcc agaagtaaaa ctcatttcta
     2941 acaatttaat gaacataaat cttacctcac tttaattttt caacaatttg tttaagtttt
     3001 gtaatctcat cacttgaaag attttcttta ttaataaaag ctgaaagaaa tttttctgaa
     3061 gaccctccaa acattttatt aattaaagtt tctgtttcct gtttttgtac ttcctctttg
     3121 cttattaaag ctttacaaac aaaattcggc tcaattctct ctattgcacc ttttgacact
     3181 aattttttta taacggtata cgtagtgttt ctattccatc ctgtttcttc ttttaaaatt
     3241 tttactaact ctccagcctt gctttctcct ctctcccata gaacttccat tactttaagt
     3301 tctgaatcaa ataattttat ctccatataa aatcgctcct caaatatagt tatcacaata
     3361 gtcattacta taataaaaaa ctatcatagt agtctatact atgtcaatca tttatttttt
     3421 aataataaaa actgtctttt taaaaaattc ttataaaaga cagttttatc aatgttatac
     3481 tttaatgttt aattaaagcc atattttatt taaatatata actatctatc ttagtacata
     3541 gtataagatt tttctttatc taatagtata tcctctacat ctgggttttc ttttactaat
     3601 tgtctataaa aattacaatt agtaacacct acatatggaa ttagccatag tagacctatt
     3661 ccaagagtag ctattgaaag tatataccat cctataaaac taaagtttag tacaaataat
     3721 ttaagtacat ttcctttcat catatctaaa ctagcaccca ttgatttaaa tacacccata
     3781 ttatccttgt cttcacaaat taagaaagta gcttgagaaa catataatag caatatataa
     3841 actggtaaca taagaacaag tattataaga actagagtta ttccagtaga aataatagtc
     3901 cctattccaa cattatcact tgataaatat tcaattgctc cactagtaag taaaactcca
     3961 aatgataaaa acgctcctat aattgtgatt ataaaagaat aaatataaac aataattgat
     4021 tcaaataaat aaaaaccaaa acttcttaaa taaattctcc cacttacaaa gatatctcct
     4081 atcttgactt tttcatcttt agtaattttc aagaacatac atgtaaatcc tgctgaaacg
     4141 caaaaagcta ttacagccac tacaatagta gtcaatggtt caaaaggtaa tcctataatt
     4201 gatattaata ctatcaatgc aatatatact gccatagaaa ttgcacatat cccccagttt
     4261 ccagataact gagctttaga tactcgtttt aattcttgcc ttgataccat aaaaaacacc
     4321 cctttcattt tataaaaaaa cttttaaaaa tataaataat cttttaacgt ataatattaa
     4381 tatatcatat tttcgactat ataagataca attattcacc atgttttatc aagatatgcc
     4441 ataattgtat
//
LOCUS       DBGJFF010000210         4405 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.215, whole genome shotgun sequence.
ACCESSION   DBGJFF010000210 DBGJFF010000000
VERSION     DBGJFF010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4405
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.215"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            231..1310
                     /locus_tag="ACOIAF_003695"
     CDS             231..1310
                     /locus_tag="ACOIAF_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HGZ2670673.1"
                     /translation="MPELLKNMYNRESLYEVAVAIQSVYNSFKVDEFIKSTMDETWNN
                     LELKARCRKISMSLGMYLPEDYKEALSILEKSVTGFYFAFFFPDFVEVYGQDDINWDL
                     SISALERNTEYWSSEFAVRAFIIKDEERMMAQMRKWSKHKSEHVRRLASEGCRPQLPW
                     GQAISKFKKDPTPVLPILEQLKTDTSTYVQKSVANNLNDISKTHPDLVISIAKDWYGK
                     NKSTNWIVKHGCRTLLKKGNRDVLALFGYDDTTSINLQDFTLETTSISIGEDLTFSFN
                     ILAKKATKTRLEYGIDYIKSNGKRNRKIFKISEVSLKENEKKSYMKKHSFADVSVRKH
                     YPGIHSIAIIINGIEKDKLDFELGI"
     gene            1634..2002
                     /locus_tag="ACOIAF_003696"
     CDS             1634..2002
                     /locus_tag="ACOIAF_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HGZ2670674.1"
                     /translation="MNVYSFWNDTLAQDAVKIRKYFHENAYINWHDTNEHFTLEEFII
                     ANCEYPDKWNGKVEKVLEFGNLVITVTHVYAINKPLSFHVVSFISLKDDKIISIDEYW
                     GEDDDVPQWRLDKHIGKPIK"
     gene            2119..2922
                     /locus_tag="ACOIAF_003697"
     CDS             2119..2922
                     /locus_tag="ACOIAF_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGZ2670675.1"
                     /translation="MEEKYILSDRGKVYYWIKESNSVFAKNLIFLPGLTANHHLFDFQ
                     IQYFSKNHNVLVWDAPTHGKSRPYLDFSYSNLVEELKVILDKQNMKKVVLIGQSAGGF
                     VAQSFIVKYPNMVEGLMMIGTCPYGTDYYSKSDIFWLKQTEWMLKLFPDKLLRNIISK
                     MCGATSEGRRSMMKMLEGYEKKELCHLMYLGFAGFIPEIQDLNIHCPVCLVVGDKDKT
                     GKVRKYNEQWHNKTGYPLHIIKNASHNVNVDKPNELNQIMDIFIKQLNI"
     gene            3307..4197
                     /locus_tag="ACOIAF_003698"
     CDS             3307..4197
                     /locus_tag="ACOIAF_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HGZ2670676.1"
                     /translation="MIESIPAKQILQKVKFDNTRWFGIDYNMNLYRGCSHGCIYCDSR
                     STIYNIENFDKVRYKENVIEILSKELRSKRKKGVVGIGAMSDTYNPFEKQLCITKQAL
                     DLISENHFGVSIDTKSSLVVRDIPILQKIKKNNSAIVKLTITTANDELSKKIEPYVNP
                     SSVRFDAVKELNDSGIFCGILLTPMLPFLTDNKDEIRAIVEKAHKANAKFIYCMYGVT
                     MRSGQREFFYEHLRDISPKLVFKYQKTYGLNYVCTVQNKDCCEKLLREECARYGILTD
                     MKEIIKAYKRSESYIQIKFF"
BASE COUNT         1683 a          478 c          827 g         1417 t
ORIGIN      
        1 cagtaaagta taaaattcta ttactataat aaaagataga actagaagtc tttgtgaaag
       61 tgggaaaagt atttatataa ataaattcat atagtttata gtttaacagt aggaaatatc
      121 gtatgagtga gaatatcgta ttttacataa agatttagca atatgacaca ctgttgtcgt
      181 gttctgtagg atatgattaa taaatcagat atgaaaggag agtgagtaat atgccagaat
      241 tactaaaaaa tatgtataat cgtgagtcac tttatgaagt tgcagtagct atccaatcag
      301 tttacaactc atttaaggta gacgagttca taaaatctac aatggatgag acatggaata
      361 acttagaatt aaaagccaga tgtcgaaaga taagtatgag tttaggaatg tatttaccag
      421 aagactataa ggaagctctt tccattcttg aaaaatcagt tacaggtttt tattttgctt
      481 ttttctttcc agattttgtt gaagtttatg gtcaggacga cataaattgg gatttatcta
      541 taagtgcatt agaaagaaat actgagtatt ggtcttcaga atttgctgta agagcattta
      601 tcatcaagga tgaagagcgt atgatggcac agatgcgtaa atggtctaag cataagagcg
      661 aacatgtacg aagacttgca agtgaaggtt gtcgtcccca gttgccttgg ggacaagcaa
      721 tatccaaatt taaaaaagac cctacacctg tgctacctat attggagcaa cttaaaacag
      781 atacatcaac gtatgtacaa aagagtgttg ctaataatct aaatgatatc tcaaaaacac
      841 atccagattt agtcataagc attgcaaagg attggtacgg taaaaataaa tccactaact
      901 ggattgtaaa gcatggttgt agaactctgc taaaaaaagg aaatcgtgat gtattagctc
      961 tttttggata tgatgatact acttctataa atcttcagga ctttacttta gaaacaacgt
     1021 ctatttctat tggagaggat ttaacttttt catttaatat tttagcaaaa aaagcgacaa
     1081 aaacaaggct cgagtacgga attgattata ttaaatctaa tggaaaaagg aataggaaga
     1141 tatttaaaat atcggaggtt tcattaaaag aaaatgagaa aaagtcatat atgaaaaaac
     1201 attcatttgc agatgtaagt gtaaggaaac attatccagg tatacactcg attgctatta
     1261 ttattaatgg tatagaaaaa gataaacttg attttgaatt aggtatataa ttttactaac
     1321 agaataatct atatcagata ctttgctaaa cttatataat atattttact aaatttatat
     1381 taggtagttt actacttgtt tactgaattt aaatacaaag aaatacaggt gctaatccct
     1441 gcccattaaa ggaatatgag cataacacaa aagtagaact tatcaattct gttacgggta
     1501 tagtagtaaa tccatagaat cacaattaga agatgaagtg taaaaaataa gttagtgttg
     1561 attttagttg ctagtgatat tgaatagtta tacaatagcc atatagatta gaatttgtag
     1621 aggagaaata taaatgaatg tatatagttt ttggaatgac actttagcac aagatgctgt
     1681 taagataaga aaatattttc atgaaaatgc ttatattaat tggcatgata caaatgagca
     1741 ttttacttta gaagaattta ttattgcaaa ttgtgagtat cctgataaat ggaatggtaa
     1801 agtagaaaag gtgctagaat ttggaaattt agtaattaca gtgacacatg tttatgcaat
     1861 aaataaacca ctatcatttc atgtcgtttc atttatcagc cttaaagatg ataagattat
     1921 ttctattgat gaatattggg gagaggatga tgatgtgcca cagtggagat tagataaaca
     1981 tattggaaag ccaataaaat agtgtttgtt ctgttttata actgaaattt tgataacatt
     2041 tgatactttg atttgtcatt ggtcaacata gtcgttagtg taataattta aaaaagtaga
     2101 atttggagag tgagcactat ggaagaaaaa tacatcttat ctgaccgagg taaagtatat
     2161 tattggataa aagaaagtaa tagtgtgttt gcaaagaact taattttttt gccaggacta
     2221 acagcaaatc atcatctttt tgatttccaa atacagtact tttcaaaaaa tcataatgta
     2281 ttggtatggg acgctcctac tcatggaaaa tcaagacctt acttagattt ctcatattct
     2341 aacttggtag aagaattgaa agtaatttta gataaacaaa atatgaaaaa ggttgtactt
     2401 atagggcaat cggcaggggg atttgtggca cagtccttta ttgtaaaata tccaaatatg
     2461 gtagaaggat taatgatgat tggaacttgt ccatatggaa ctgactatta tagtaagtca
     2521 gatattttct ggttaaaaca gactgaatgg atgttaaagt tattcccaga taaattgttg
     2581 cgaaacataa tatctaagat gtgtggagct acgtctgaag gcagaaggag tatgatgaaa
     2641 atgttagaag gctacgaaaa aaaagaactg tgtcatctca tgtatctggg atttgctggg
     2701 ttcataccag aaattcaaga tttaaatata cattgtccag tttgtttggt ggttggagat
     2761 aaagacaaga cgggtaaagt gagaaaatat aatgagcagt ggcacaataa gactgggtat
     2821 ccattgcaca ttataaaaaa tgcttcacat aatgtaaatg tcgataaacc taatgaacta
     2881 aatcagatta tggatatatt tattaagcaa ttaaatatat aaaaactaca tactcattag
     2941 gtaaattatg tagtatatgt gtttacagat ggttatagaa ataaatatgt aataaactac
     3001 ttaacactta aaaagtggat ttgtccagag cataatacaa agcatgatat ataaattttt
     3061 aagcgaaata catcaaaaga aggattagaa ttagtggaga taaaacagct attagagatt
     3121 ataaataaat agttttaaag gtactacgaa tatagaggaa ggtaaatcat acgttattca
     3181 aggattataa atgattagtt taaaagtact atcggatagg tctctgcata tttaaacctt
     3241 agtaaaggta gttaaagtag tagtttaaaa actaaaaaat tatataaagg aattaggtat
     3301 aagaaaatga ttgagagtat accagcaaaa caaattttac aaaaagtgaa atttgacaat
     3361 acaagatggt ttggaattga ctataatatg aatctctatc gtggttgttc tcatggatgt
     3421 atatattgtg atagccgtag cacaatatac aacatagaga attttgacaa agttagatat
     3481 aaagaaaatg tgatagaaat tttaagtaaa gaattgcgtt caaaaagaaa aaaaggggta
     3541 gtaggcattg gagctatgtc tgacacatat aatccttttg aaaagcaact ttgtattaca
     3601 aagcaagcac tagatttgat ttcggaaaat cattttggag tatctattga tactaaaagt
     3661 agtttggtgg ttagagatat accgattttg cagaaaatta aaaaaaataa ttctgctatt
     3721 gtaaagttga ctataaccac tgcaaatgat gaattatcaa agaaaataga accttatgta
     3781 aatccaagtt ctgtacgatt tgacgcagtg aaagaactta atgattctgg aattttttgt
     3841 ggcatattat tgactcctat gcttccattt ttgactgata ataaagatga aatacgagca
     3901 attgtagaaa aagcacacaa ggctaatgca aaatttattt attgcatgta tggcgtaaca
     3961 atgcgtagtg gtcagagaga gtttttttat gagcatttaa gagatatatc accaaaattg
     4021 gtatttaaat atcagaaaac atatggttta aactatgtat gtactgtaca aaataaagat
     4081 tgttgtgaga agttgttaag agaagaatgt gcacgttatg gtattttgac ggatatgaaa
     4141 gagattataa aagcgtacaa gaggtcggag tcatatattc aaataaaatt tttttaagat
     4201 aggctttgga tgagatataa tagtggtgat tgatgtgttg atattatagt tgattgttag
     4261 agaggatatt atagatgaat attagtattg atattctagg taattgctag agctgatatt
     4321 atagatgaat attaatattg atattctagg taattgctag agttagtatt atagatgaat
     4381 attagtattg atattttagg taact
//
LOCUS       DBGJFF010000211         4375 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.216, whole genome shotgun sequence.
ACCESSION   DBGJFF010000211 DBGJFF010000000
VERSION     DBGJFF010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4375
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.216"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            <1..282
                     /locus_tag="ACOIAF_003699"
     CDS             <1..282
                     /locus_tag="ACOIAF_003699"
                     /inference="COORDINATES: protein motif:HMM:NF014225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670677.1"
                     /translation="NSLRENGIEADIVNRVEESRPNILDAIRNKQVDIVINTPTKGND
                     STRDGFKIRRTAIEFSTEIMTSLDTLKALVEVKKKHLNKDELKVYNIAE"
     gene            540..1589
                     /gene="carA"
                     /locus_tag="ACOIAF_003700"
     CDS             540..1589
                     /gene="carA"
                     /locus_tag="ACOIAF_003700"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002845470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HGZ2670678.1"
                     /translation="MKARLILEDGTVFIGKAFGYLEESVGEVVFNTSMIGYGEVLTDP
                     SYYGQIVTMTYPLVGNYGINLSSAESEKVQVKGFIVREKSDSPSNFRCEIDIDQYLKQ
                     NKVIGLEGIDTRALTKILRNNGTMKGIITLEDNKLEDVKHKLDKFSNTEAVRTVTRKE
                     VEHIKGNGPKVAVMDFGVKRNILRSFIARGCDITIFPATTSPEDVLSINPDLIFLSNG
                     PGDPEDLEDVIENIKALIGKKPIVGICLGHQLLALALGGKTAKLKFGHRGGNHPVKDL
                     EEGKVFITSQNHGYYVSEVPEQMKVTHINLNDNTVEGMRHEKLDVYSVQYHPEACPGP
                     KDNDYIFDKFLELVK"
     gene            1782..>4375
                     /gene="carB"
                     /locus_tag="ACOIAF_003701"
     CDS             1782..>4375
                     /gene="carB"
                     /locus_tag="ACOIAF_003701"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) large subunit"
                     /protein_id="HGZ2670679.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQALKEEGIEV
                     VLVNSNPATIMTDKEIADKIYIEPLTIEFIEKIIEKERPDSLLAGMGGQTGLNLAVEL
                     HDAGILDKYNVKVIGTSIESIKKGEDRDLFREVMKEINQPVIVSDIVTNLEAGLEFAN
                     KIGYPVVVRPAYTLGGTGGGIADTEEELREILSHGLQLSPVGQVLLEKSIKGWKEIEY
                     EVMRDGNGNCITVCNMENVDPVGVHTGDSIVVAPSQTLSDEEYQLLRKASIDIINAIE
                     VQGGCNVQIALNPHSLEYAIIEINPRVSRSSALASKATGYPIAKVAAKIALGYTLDEI
                     ENAVTKKTYACFEPTLDYVVVKIPKWPFDKFRHANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHAPSEGRTLEELKARVVVPDDERLFDLAEMIRRGYKVEMISE
                     ITGVDKWFINKFKWIVEQEEKLKVLKIEDLDKEYLHELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIQPVYKMVDTCGGEFEALSPYYYSTYEQYDEVVVSDKRKVVVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMGIETIIVNNNPETVSTDFDTSDKLYFEPLTEEEVLNI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFRDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWTTNEGVEIAKELGYPVLVRPSYVLGGQGMEITYNEEKLIQYLDDAFDRD
                     HKNPVLIDKYLTGREIEVDAICDKEDILIPGIMEHLERAGVHSGDSTTMYPSQNISDE
                     IKEKIVEYTKKIALDLNVLGMVNIQFIEFQNELYIIEVNPRASRTVPYISKVSGVPIV
                     DLATKCMLG"
BASE COUNT         1806 a          445 c          865 g         1259 t
ORIGIN      
        1 aatagcttaa gagaaaatgg aatagaggct gatatagtaa atagagtaga agaatctaga
       61 ccaaacatat tagatgctat aagaaataaa caagttgata tagtaataaa tacaccaact
      121 aaaggaaatg actctacaag agatggattt aagataagaa gaacagctat tgaattttct
      181 actgaaataa tgacatcttt ggatacacta aaagctttag tagaagttaa gaagaaacat
      241 ttaaataaag atgagttaaa agtttacaat atagctgaat agaaattaag ttgattgtat
      301 agtttaagaa tttaattatt aaataaaaaa aaagaggtta ttttttctag gagtttttac
      361 ttttgagata acttcttttt tttaagaaat ttgtagtata atagaaaaag ataaaaaata
      421 aatgtaaata agtataaaac tagaatgtaa aagtgaaaaa tagatataga tatttaaaag
      481 cttatattta gttaatgttc tttagtttgt ctaaagagaa ttataggagg tactgtagta
      541 tgaaagcaag gttaatatta gaagatggaa cagtttttat aggaaaggca tttggatatt
      601 tagaagaaag tgtaggagaa gtagtattta atacttcgat gataggatat ggtgaagttt
      661 taacagaccc ttcttactat ggtcaaatag ttactatgac ttatcctctt gtaggaaatt
      721 atggtataaa tttatcatca gcagagtctg aaaaagttca agtaaaggga ttcatagtaa
      781 gggagaaaag tgattctcca agtaatttta gatgtgaaat tgatatagac caatatttaa
      841 agcaaaataa agttatcgga cttgaaggaa tagatacaag agctttgact aagatactta
      901 gaaataatgg aacaatgaaa ggtataatta cattagaaga caataaatta gaagatgtta
      961 aacataaatt ggataagttt tcaaatacag aagcagttag aactgttaca agaaaagaag
     1021 tagagcatat aaaaggaaat ggtccaaaag tggctgttat ggattttgga gtaaaaagaa
     1081 atatattaag atcatttata gctcgtggat gtgatataac aatatttcca gctacaacat
     1141 ctccagaaga tgtattgagt ataaaccctg acttaatatt cttgtcaaat ggacctggag
     1201 acccagaaga cttagaagat gttatagaaa atataaaagc tcttattggt aaaaaaccta
     1261 tagttggaat atgcttagga catcaacttt tagctttagc tcttggagga aaaactgcta
     1321 aacttaaatt tgggcataga ggtggaaatc atccagttaa agatttagaa gaaggaaaag
     1381 tatttataac ttctcaaaat catggttatt atgtttcaga agttcctgaa caaatgaaag
     1441 tgacacatat aaatttaaat gataatactg ttgaaggaat gcgacatgag aagctagatg
     1501 tatacagtgt tcaataccat cctgaagctt gcccaggacc aaaagataat gactatattt
     1561 ttgataaatt tttagaatta gtaaaataaa taacattaga atagctaact aaaaaacaag
     1621 aataacttaa atcaaaaagt ttggtttaat taacatagat gtattaaata gatgtattga
     1681 tgagtaaatt tgaacttaag ataaaataaa gtaattaaag ttgattgtat agatgatgaa
     1741 gataagaact gtgaaaattg tttagataag gagaaagagt tatgcctaaa ttagatagta
     1801 taaagaaaac tttagtatta gggtcagggc caataataat aggtcaagct gcagagtttg
     1861 actattcagg aacacaggcc tgccaagcat taaaagaaga gggaattgaa gttgtattag
     1921 taaatagtaa cccagctact ataatgactg ataaagaaat tgcagataaa atatatatag
     1981 aaccattaac aatagaattt atagaaaaaa taatagaaaa agaaagacca gacagtttac
     2041 ttgcaggtat gggtggtcag acaggattaa acttagctgt agaattacat gatgctggta
     2101 tattagataa atataatgta aaagttatag gaacatctat agaatctata aaaaagggcg
     2161 aagatagaga tttatttaga gaagtaatga aagaaataaa tcaaccagta atagttagtg
     2221 atatagttac aaatttagaa gcaggtcttg agtttgccaa taagatagga tatccagtag
     2281 ttgtaagacc agcatatacg ctaggtggaa ctggtggagg tatagctgat actgaggaag
     2341 aattaagaga aatactttca catggactac agctaagtcc tgttggacaa gttcttcttg
     2401 aaaagagtat aaaaggttgg aaagaaatag aatatgaagt aatgagagat ggaaatggaa
     2461 actgtataac tgtatgtaat atggaaaatg tagacccagt tggagttcat acgggagaca
     2521 gtatagttgt tgctccaagt caaactctaa gtgatgaaga gtatcaacta cttagaaagg
     2581 catcaataga cataataaat gcaatagagg ttcaaggtgg atgtaacgtt caaatagctt
     2641 taaacccaca tagtttagaa tatgcgataa tagaaataaa cccaagagtt agtaggtcat
     2701 cagccctagc atcaaaagct acaggatacc caatagcaaa agtagcagct aagatagctt
     2761 taggttatac tttggatgag atagaaaatg cagtaactaa aaaaacttat gcttgttttg
     2821 agccaacatt agattatgtg gtagtaaaaa taccaaagtg gccatttgat aaatttagac
     2881 atgccaatag aaaattagga actaagatga tggcaactgg tgaaatcatg agtataggaa
     2941 gtaactttga ggctgctatt cttaaaggta taagatcact tgaaacagga aaatattcat
     3001 tggttcatgc tccatcagaa ggtagaacat tagaagaatt aaaggctaga gtagtagtac
     3061 ctgatgatga gagactattt gacttagctg aaatgataag aagaggctat aaagtagaaa
     3121 tgatatcaga aataactggt gttgataagt ggtttataaa taaatttaaa tggatagtag
     3181 aacaagaaga aaagttaaaa gttctaaaaa tagaagactt agataaagaa tatttacatg
     3241 aattaaagaa aaagggattt tctgataaag gtatatctga cttaatgaag ataagcccag
     3301 aaaaattata tgaattgaga agtttatata atatacaacc agtttataaa atggttgata
     3361 catgtggagg agaatttgaa gctctatcac cttattacta ttcaacttat gaacaatatg
     3421 atgaggtagt agttagtgat aagagaaaag ttgtagtatt gggttctggt ccaataagaa
     3481 taggtcaagg aatagaattt gactattgtt cagttcactg tgtaaaatct ttaagaaaaa
     3541 tgggaataga aactataatt gtaaacaata accctgaaac tgtaagtacg gattttgata
     3601 catctgataa gttgtatttt gaaccattaa cagaagaaga agttttaaat ataatagaaa
     3661 aagaaaaacc agaaggtgta atactacagt ttggtggaca aacagctata aaattggcta
     3721 agtttttaca tgaaaaaaat atacctatac tgggaacaga ctttagagat atagatgcag
     3781 cagaagatag agaaaaattt gatgatttac tagagagact agatataaat agaccaaaag
     3841 gaaaaggtgt atggacaact aatgaaggtg ttgaaattgc taaggagtta ggttatccag
     3901 tattagttag accatcttat gtacttggtg gtcaaggtat ggaaataact tataacgaag
     3961 aaaaactaat tcagtatttg gatgatgcat ttgatagaga ccataaaaat ccagtactta
     4021 tagataaata tctaactggt agagaaatag aagtagatgc tatatgtgat aaggaagata
     4081 tattgatacc tggtataatg gagcatttag aaagagctgg agttcactct ggagatagta
     4141 caacaatgta tccaagtcaa aatatatctg atgaaataaa agaaaagata gtagaatata
     4201 ctaaaaaaat agctttagac cttaatgtgc ttggtatggt caatatacag tttatagaat
     4261 ttcaaaatga attatatata attgaggtta accctagagc tagtagaaca gttccatata
     4321 taagtaaggt aagtggagta ccaatagttg acttggctac taagtgtatg ttagg
//
LOCUS       DBGJFF010000212         4288 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.217, whole genome shotgun sequence.
ACCESSION   DBGJFF010000212 DBGJFF010000000
VERSION     DBGJFF010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4288
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.217"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(257..2644)
                     /locus_tag="ACOIAF_003702"
     CDS             complement(257..2644)
                     /locus_tag="ACOIAF_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HGZ2670680.1"
                     /translation="MEANNSIKKEFILGGLNCAHCAEEINNKVSKLQEVKSSNLNFIN
                     KKLTVNIKESFNEDTTIEKIIDIIDSTEPGLDIQISSKENASSKTSIKKELILGGLNC
                     AHCAEEINNKVSKLKEVESSNLNFVNKKLTINISNNFKEDDVINKIKEIINSTEPGLD
                     IQVKSTDKVKGRTTEKSGAVNDTNKKELIPLIIGALVYIFGIYQTATGYESQFSNIVF
                     IVAYVIVGGDVLLRAIRNISKGRVFDENFLMALATVGALAIGELSEAVGVMLFYKVGE
                     YLQGVAVGKSRKSITSLMQIRPDYANLKVNSEVKVVSPEEVNVGDIIVVKPGEKVPLD
                     GVVVDGVSMLDTSALTGESVLREVEKGDEILSGVINKNALLSIEVTKSFGESTVSKIL
                     DLVENSSIKKSKTENFISKFSRYYTPIVVIAALLIAFVPPLVISGEVFSDWLYRGLIF
                     LVVSCPCALVLSIPLSFFSGIGFASKNGILIKGSNYLEALRSVDTVVFDKTGTLTKGV
                     FNVTKLNPEGISEEELLEYAAIAEVNSNHPIAKSILSYYNKKIDLDTIDSYEEIAAYG
                     IRVKHNGNFILAGNEKLMKKENISYSSAKEVGTVVYIAVDKVYRGYIVISDEVKEDSK
                     NAIRSLKAIGVKEVVMLTGDNEKVAKNIAQELELDTVYSNLLPNEKVDRLEDLYEGRT
                     EKEKIAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVLMTDEPSKISKAIEIA
                     NKTNKIVWQNIIFALGVKIIVMILGAGGVATMWEAIFADVGVALIAVVNAMRAMR"
     gene            complement(2667..3032)
                     /locus_tag="ACOIAF_003703"
     CDS             complement(2667..3032)
                     /locus_tag="ACOIAF_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HGZ2670681.1"
                     /translation="MREEINDCNCNIVHEEIVTEAKSTMPDEEMLYDLAELFKVFGDT
                     TRVKILYALFANEMCVCDIASLLNMTHSAISHQLRVLKQARLVKFRREGKTVYYSLDD
                     SHISQIFDCGLNHIRETYK"
     gene            3231..4034
                     /locus_tag="ACOIAF_003704"
     CDS             3231..4034
                     /locus_tag="ACOIAF_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670682.1"
                     /translation="MSNNKKKDLDTSYMPYLDPANYADYITNEEIPQDELRNDPQISN
                     GYTKIPNNQNINNLYPNNVNHYPKSSNSPNNLPNTNNTSGNMNYNTSATNNMPNNMNN
                     NTGMPNNMPNNMNSNMGMPNNMPNNMNSNMGMPNNVPNNMNSNMGMPNNMSNNMNSNM
                     GMPNNMSGNMNNMYGDTGMPNNMNSNMGYNMNNTMGIPNFSCNQNMPPNVLMMIPGVI
                     CHNTMQGMPVVMPSTMPPNIYPTPYGSSNMSVQGIPQATNIEEFDEEEM"
BASE COUNT         1433 a          768 c          473 g         1614 t
ORIGIN      
        1 aatctaattt aaacttaata taaatttatt aataaacctt ctatgtctaa attttaaaat
       61 tacaatataa aagctctaca ctaaataaat acattaaata aacataatgg ataatgaata
      121 taaatttata tatgcaaaag cactccaata aaaaggtacc aataaagtta aaagaaactt
      181 attgatacct ttcattatta catacttcaa tatataatta taagtttttt gaatagtatg
      241 gatttacatt tatcccttat ctcatagctc tcatagcatt tactactgct ataagtgcca
      301 cacctacatc agcaaatata gcttcccaca tagtagctac tcctccagca cctagtatca
      361 taactataat ttttactcct agagcaaata ttatgttttg ccacactatc ttattagtct
      421 tgtttgctat ttcaattgct ttagatatct tgctaggctc atctgtcata agcactacat
      481 ctgcagcttc aatagcagca tctgaaccta agcctcccat agctatacca acgtcagcac
      541 gagctaatac tggagcatca tttattccat cacctacaaa tgctattttc tctttttcag
      601 ttcttccttc atataaatct tctaatctat ctaccttttc attaggaagt aaatttgagt
      661 aaactgtatc caattctaat tcttgagcaa tattttttgc tactttctca ttatctccag
      721 ttaacattac aacttctttt acccctattg cttttaagct tcttatggca tttttactat
      781 cttcttttac ttcatcagat ataactatat aacctctata tactttatca acagcaatat
      841 agactactgt gcctacttct ttagctgatg aataagatat attttctttt ttcataagtt
      901 tctcattacc tgctaatata aaattgccat tatgctttac tctaattcca taagctgcta
      961 tttcctcata actgtctatt gtatctaaat caattttttt attatagtaa cttaatatag
     1021 actttgctat tggatgattt gaatttacct ccgcaatagc tgcatattct aataattctt
     1081 cttctgatat cccttcagga tttaacttag ttacattgaa cacaccttta gtaagtgttc
     1141 ctgttttatc aaatactact gtatctacac ttcttaaagc ttctaaatag ttacttcctt
     1201 taataaggat accattttta gatgcaaacc caattccact aaagaaacta agtggtattg
     1261 ataaaactaa tgcacaaggg caagaaacga ctaagaatat caatcctcta tataaccaat
     1321 cactaaatac ctcacctgag attacaagtg gtggcacaaa tgctattaat aaagcagcta
     1381 ttactactat cggagtatag tatcttgaaa acttagatat aaagttttct gtctttgatt
     1441 ttttaatgct agaattttca acaaggtcaa gaatcttaga aactgttgat tctccaaaac
     1501 tctttgtaac ttcaatactc aataaagcat ttttatttat aacacctgat aagatttcat
     1561 ctcctttttc aacttctctc agcactgact ctcctgtgag tgcagaagta tctaacatag
     1621 aaactccatc tactactaca ccatctaatg gtactttttc accaggtttt actactatta
     1681 tatcacctac atttacctct tctggtgaca ccaccttaac ttcagaatta acttttagat
     1741 ttgcatagtc tggtcttatt tgcataagtg aagttataga ctttctggat ttaccaactg
     1801 caacaccttg taaatactct cctactttat agaaaagcat aacccctact gcttctgata
     1861 actcccctat tgctaatgct cctactgttg ctagagccat caaaaagttc tcatcaaata
     1921 ctcttccttt tgatatattt cttattgctc ttaaaagcac atcaccacct actattacat
     1981 aggcaactat aaatactata ttgctaaact gactttcata acctgtagct gtttgatata
     2041 taccaaatat atatacaagt gctccaatta taagtggaat taattctttc ttatttgtat
     2101 catttacagc tcctgatttt tcagtagttc tcccttttac tttgtcagta gactttactt
     2161 gtatatcaag ccctggctct gttgaattta ttatttcttt tattttattt ataacatcat
     2221 cctctttaaa gttattactt atatttattg taagtttttt atttacaaag tttaaattag
     2281 aagattcaac ttcttttaat ttagaaacct tgttattaat ctcctctgca caatgtgcac
     2341 agtttaatcc tcctaatata agctcttttt taattgatgt tttactagat gcattttctt
     2401 tagaacttat ttgtatatca agacctggct ctgttgaatc tattatatct attatctttt
     2461 ctatagtagt atcctcatta aagctttcct ttatatttac tgtaagtttt ttatttataa
     2521 aatttaaatt agaagattta acttcttgta gtttagaaac tttattatta atctcttctg
     2581 cacagtgagc acaatttaat cctcctagta taaattcttt tttaattgaa ttatttgctt
     2641 ccatatatat tcactcctct catttatcat ttataagtct ctctaatatg atttaatcca
     2701 caatcaaata tctgacttat atgactatca tctaatgagt aatacactgt ttttccttct
     2761 cttctaaact ttactaatct agcctgcttt aatactctaa gttgatgaga tattgcagag
     2821 tgagtcatat ttaataaact cgctatatca catacacaca tttcgtttgc aaataatgca
     2881 tataatatct ttactctagt agtatcccca aatactttaa ataattctgc taaatcatat
     2941 aacatctctt catctggcat agttgattta gcctctgtta ctatttcttc atgtactata
     3001 ttacaattac aatcatttat ctcttctctc atttttaatc ctccattatt ttaatatatg
     3061 aataactgtt catatgttca ttatatgcta tcatttctaa atttgcaaca ataattttta
     3121 ttaatttaat ataattagaa tgcacatatt ataaactttt ttaatatata tattattaga
     3181 ataagttttt gtaaactttt ttatagtttt ctaggaggtg caagtttaat atgagtaaca
     3241 ataaaaaaaa ggatttagat actagttaca tgccttactt agacccagcg aactatgcag
     3301 attatattac taatgaagaa attccccaag atgaattgag aaatgaccct caaatttcta
     3361 atggttatac aaaaatacct aataaccaga acataaataa cctatatcct aataatgtaa
     3421 atcattatcc taaaagtagt aattctccaa acaacttgcc taacacaaat aatacatccg
     3481 gaaatatgaa ttacaataca tctgctacaa ataatatgcc taataacatg aataataata
     3541 cgggtatgcc taacaatatg cctaataaca tgaatagtaa catgggtatg cctaacaata
     3601 tgcctaataa catgaatagt aacatgggta tgcctaacaa tgtgcctaat aacatgaata
     3661 gtaatatggg tatgcctaac aatatgtcta ataatatgaa tagtaatatg ggtatgccta
     3721 acaacatgtc tggtaatatg aataatatgt atggtgatac aggtatgcct aataacatga
     3781 acagtaatat ggggtataat atgaataata ctatgggtat tcctaacttc tcatgtaatc
     3841 aaaatatgcc tcctaatgta cttatgatga taccaggagt tatatgtcat aatacaatgc
     3901 aaggtatgcc tgtagttatg cctagtacta tgcctcctaa tatatatcct actccttatg
     3961 gttctagtaa tatgtctgta caaggtatac cacaggctac taatattgaa gaatttgatg
     4021 aggaagaaat gtaatatata tttttttaag taagttgtat aaaacattaa taattgacca
     4081 taataataaa tgtattcctc ataatattcc cccaatatta ttataggtat agaaagttga
     4141 ctgaatatgc gcttattcgg tcaacagata ttaacataat ctaataagat aaacatagaa
     4201 aaagtcagat aaaatttaat tttatctgac tttttcattt aaaaatgctc tcttgtgttg
     4261 gtgattcaga atagtatggt actcgtat
//
LOCUS       DBGJFF010000213         4255 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.218, whole genome shotgun sequence.
ACCESSION   DBGJFF010000213 DBGJFF010000000
VERSION     DBGJFF010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4255
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.218"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(246..1127)
                     /gene="pepI"
                     /locus_tag="ACOIAF_003705"
     CDS             complement(246..1127)
                     /gene="pepI"
                     /locus_tag="ACOIAF_003705"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase"
                     /protein_id="HGZ2670683.1"
                     /translation="MKITEGYMPFKGFKTYYRIVGENTEGKKPLVLLHGGPGSTHNYF
                     EVLDKIAESGRQVIMYDQIGCGNSFVEGHPELFNADTWIEELIELRKHLGLDEIHLLG
                     QSWGGMQAIWYAIEYKPKGIKSYILSSTLSSAKLWEKEQKRRISYMSEADQKALLDAV
                     NTGDYSSKEYNDALERFMEMYCAGEVTEDSPECLRRPKKSGSEAYIVGWGQNEFSPTG
                     TLSGYEFTDRLHEIKEPCLVTSGAIDLCSPYIAKTMYDRIPNSKWELFEYSRHMPFVE
                     ENEKYIKVLTEWLNAND"
     gene            1583..2557
                     /locus_tag="ACOIAF_003706"
     CDS             1583..2557
                     /locus_tag="ACOIAF_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HGZ2670684.1"
                     /translation="MNNINSLKVIKLSLGCSLAIFIAWLLKLEYSMVAGVIVLLTVKD
                     TKRETLKGSIGKIYGFLLCTIFSYLCFNILGYNLTSFSIFIFIVIPLCFLLNIQDVIA
                     MCVVIASHYYLQGETSIKWILNEAGIFAVGTGIGVLINMYIPTNIHKIHEVQKKLQEE
                     VSIVLIDIADIIVNPKKENGYSRDLYTLNSLIDSSISETYDNINNTLLSDTRFFLEHM
                     DIIKSQRDILENLYSYVSQLNSTPPQAHILSAFIHKIGYTEFEAETGNLLLEELKRLM
                     LSMKNQPLPVDRTEFENRAILFLCLTELKQFLVNRKHAQMLRDNNFYK"
     gene            complement(2673..3734)
                     /locus_tag="ACOIAF_003707"
     CDS             complement(2673..3734)
                     /locus_tag="ACOIAF_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HGZ2670685.1"
                     /translation="MKKIRKLATKLLITTIVLISGMSMTVYGMTAKEVTAKTPKSYVT
                     GTNSVYGPKLSQAQLNSVAQATADFMNKKITKNMTTDAKILVAYNHIKNNTTYVDWNA
                     VEGANTAYTLVTKKGACSGMARSMKALCDAMGIESYYVHSTSNDHQWNLIRFGDGRLY
                     HVDIDANKSAGKDIIYKSLSHPLPFDKTAYPKVGAIVQTVKPEQTQKPAPPGKHTMLP
                     HQEGAKAVGNFGKKGEDINEKHVMYEGVRCSVVNSSVNTITFTGSESNLDPSNLTIAV
                     IGFMNTPYGMFALAPEGKPVEYPFKYDKPFSLDFSGKNRSGAELAKYCKKNDVTIKIE
                     ITDEKANIGLGQIMYVRYE"
BASE COUNT         1419 a          691 c          548 g         1597 t
ORIGIN      
        1 aaaaaatttc taaaaaacaa taaaattaag ttataaacac caatatattt ctattatttc
       61 aataagtata caattagatt agaatctaac gctagtaata aaaaattact ttcccaaaat
      121 agatataagc ttcattttat cgttcaaata atgaaaatat atttgatttc atgcaaaaag
      181 agtgcctaat gaactaaaaa gttcattttg agacactcat ttttttatat aaatttatat
      241 agtttttaat cgttcgcatt caaccactca gttaagactt ttatatattt ctcattttct
      301 tcaacgaatg gcatatgtct tgaatactca aataattccc acttactatt aggtattcta
      361 tcatacatag tctttgcaat atatggtgaa cataaatcta ttgctccact agttactaga
      421 caaggctctt ttatttcatg taatctatct gtaaattcat atccagaaag agttcctgta
      481 ggagaaaatt cattttgtcc ccatcctaca atatatgctt cagaacctga ttttttaggt
      541 cttctcaggc actctggaga atcttctgtc acttcacctg cacaatacat ttccataaat
      601 ctttctaatg catcattata ttctttgcta gagtaatctc cagtatttac tgcatcaagt
      661 aatgcttttt ggtcagcctc actcatatat gatattcttc ttttttgttc tttctcccaa
      721 agttttgcag aagaaagagt agatgaaagg atgtaagact taataccttt tggtttatat
      781 tcaatagcat accaaatagc ctgcatacct ccccaagatt gacctaacag atgaatttca
      841 tcaagtccta gatgctttct tagttcaata agctcctcta tccaagtatc tgcattaaat
      901 agctcaggat gcccttctac aaatgaattt ccacaaccaa tttggtcata cattattact
      961 tgtctaccac tttcagctat cttatccaat acctcaaaat agttgtgtgt agaacctgga
     1021 cctccatgaa gtaatactaa tggcttcttc ccttccgtat tttctcctac tatacggtaa
     1081 tacgttttaa aacctttaaa aggcatatat ccttcagtaa ttttcatgtt tatctcctcc
     1141 aatttgtaat tataaacaat aatcaagttc attaacaata ctatttaaaa cctcttatac
     1201 atatttgatt atgtatttta atctttatac ctacttttat aaatagtata acataaaagt
     1261 taaaaatctc tatactaaac caattataaa attatattaa ctatataatt ttatttttta
     1321 ataatagtat atacatattt gattattttc atttgctgct taaaatcaat tttaagtttt
     1381 cttcctataa taaacttcct ctttgtggtc tcatgttagt ctctaaattt aaaattttac
     1441 aaaaaatttt cataaaataa aattatattg attgtatcta aacaaatagg gcaataaaat
     1501 aattaagtat agttacttac ctttcaagca atattagttt tatgttagaa ttttattata
     1561 ttgatttaca ggaggtttgc atttgaataa tattaattca cttaaagtaa taaaattatc
     1621 tcttggatgt tctcttgcta tatttattgc ttggctatta aaattagaat attcaatggt
     1681 tgctggagtt atagtactac ttacagtaaa ggacactaaa agagaaactc ttaaaggctc
     1741 cattggaaag atttatggtt ttcttttatg tactattttt tcatatttat gttttaatat
     1801 tttaggatat aatttgactt cattttcaat atttatattt atagttatac cactttgttt
     1861 tttgttaaat attcaagatg ttattgctat gtgtgtagtc atagcatccc attactacct
     1921 tcaaggagaa acttctatca aatggatttt aaatgaagct ggaatatttg cagtaggaac
     1981 tggaataggt gtcttaataa atatgtatat tccaacaaat atacataaaa tccatgaagt
     2041 tcaaaaaaag ttacaggaag aagtgagtat tgtacttata gacattgcag atattattgt
     2101 aaatcctaaa aaagaaaatg gatatagtag agatttatat acattaaatt cacttattga
     2161 cagcagtatt tctgaaactt acgataatat taataatact ttattaagtg atactagatt
     2221 cttcttagaa catatggata ttataaaaag ccaacgggat atcttagaaa atttgtatag
     2281 ttatgtatct caactaaatt ctacaccacc acaagctcat attctatctg cttttataca
     2341 taagataggt tatactgaat ttgaagctga aacaggcaat cttcttcttg aagaattaaa
     2401 gagactaatg cttagtatga aaaaccagcc tctacctgtt gacagaacag agtttgaaaa
     2461 tagagctatt ttattcctat gcttgactga gttaaaacag tttttagtta atcgtaaaca
     2521 tgctcagatg cttagagata ataattttta taaataaaaa ggggagaaat ctagcagttg
     2581 acctgactac aaaatatcag atataatatt aaatgcgccc ctagtaagag ggcgcaatgt
     2641 ttataactta ttctactgtc atgattaaat cattattcat atcgaacata cattatttga
     2701 ccaagtccaa tattagcttt ttcatcagtg atttctattt taatagtcac atcatttttt
     2761 ttacagtact tcgctaattc tgcaccagaa cgatttttac cactaaaatc taaagaaaat
     2821 ggcttatcat atttaaaagg atattcaaca ggtttaccct ctggtgctaa tgcaaacatc
     2881 ccatatggtg tgttcataaa tcctattaca gcaatcgtaa gatttgatgg gtctaaattt
     2941 gattcactac ctgtaaatgt aattgtattt actgatgaat taactacact acatctaaca
     3001 ccttcataca ttacatgttt ttcatttata tcttctcctt tttttccaaa atttccaaca
     3061 gcctttgccc cttcttgatg tggtaacata gtgtgcttcc caggtggtgc tggtttctgt
     3121 gtctgttctg gttttactgt ctgtactatt gcaccaactt ttggatatgc tgtcttatca
     3181 aatggtaatg ggtggctaag actcttgtat atgatatcct ttcctgctga tttattagca
     3241 tcaatatcta catgatacaa acgcccatca ccaaaacgga taagattcca ctggtggtca
     3301 tttgatgtgc tgtgtacata ataagattca attcccatag catcacacaa agctttcatg
     3361 ctcctagcca ttcctgagca tgcacctttt ttagtaacca atgtataggc agtatttgct
     3421 ccctctactg cattccagtc aacatatgtt gtgttatttt taatgtgatt gtaggcaact
     3481 aaaatcttag catctgtggt catattcttt gtgattttct tgttcataaa atctgctgtt
     3541 gcttgtgcta ctgagtttag ttgggcttgt gacagctttg gaccatatac gctattggtt
     3601 ccagtgacat agctttttgg tgtttttgct gttacctctt tggctgtcat accataaact
     3661 gtcatactca tacctgagat taaaactatt gttgtaatca ataacttggt agctaacttc
     3721 ctaattttct tcatacttaa tattctcctt tataaaacat tgttacttcc tattctctac
     3781 cattaataaa attaatttag gatttaaaaa ggtggattgt caacatgtat atttctccat
     3841 tcatacattc gtgaaatatt taaatttcat ggaaaatcta atagatattt tctagataat
     3901 ttgtatacaa tacaactcgc aaatcagggt atttgctgtt agtttgagaa aatgatgatg
     3961 attaatacat tcggaatctt tgacctataa taaataccat tgtcttcaat cattaataat
     4021 ggtaagagta aagagaatta tcaataatac agttaaaatc aactttgtat acatttcata
     4081 cattctctcc caacaacatt atacttctta taatttgtat attcaatacg ttttctggga
     4141 aaatatagga tatatctata aaaatataaa aaaggagttg aacttaaaat ttgattaaaa
     4201 aaaatcaatt ttaagacaac tcatttgtat tataaaaatg ctatatatta ttatt
//
LOCUS       DBGJFF010000214         4181 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.219, whole genome shotgun sequence.
ACCESSION   DBGJFF010000214 DBGJFF010000000
VERSION     DBGJFF010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4181
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.219"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            224..979
                     /gene="dapB"
                     /locus_tag="ACOIAF_003708"
     CDS             224..979
                     /gene="dapB"
                     /locus_tag="ACOIAF_003708"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HGZ2670686.1"
                     /translation="MLKVIISGYNGTMGRVLAECVKKDNELELVCGVARSADEKGHHS
                     DTKLYKTMTDVTEKTDVIIDFSHHETLEPILSYALKNNTPVVLATTGYDENDLNKIEE
                     ASKNIPILLSSNTSLGVNILIKLVKDASKMLEGFDIEIIEKHHNRKEDAPSGTATMIA
                     NAIREVLPESENTHGRYGRDCKRKPNEIGIHSIRGGNIISDHDVIFAGDNEVLTLSHH
                     SQSDEVFANGSIVAAKFLIGKQSGLYKMSDILG"
     gene            complement(1333..3036)
                     /gene="bclA2"
                     /locus_tag="ACOIAF_003709"
     CDS             complement(1333..3036)
                     /gene="bclA2"
                     /locus_tag="ACOIAF_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA2"
                     /protein_id="HGZ2670687.1"
                     /translation="MSDISGPSLYQDVGPTGPTGATGPTGPTGPRGATGATGANGITG
                     PTGNTGATGANGITGPTGNMGATGPNGTTGSTGPTGNTGATGANGITGPTGNTGATGA
                     NGITGPTGNKGATGANGITGSTGPTGNTGATGANGITGPTGNTGATGATGPTGLTGAT
                     GATGANGITGPTGNTGATGANGVTGATGPTGNTGATGPTGSIGATGATGTTGATGPIG
                     ATGPTGADGEVGPTGAVGATGPDGLVGPTGPTGPTGATGANGLVGPTGPTGATGANGL
                     VGPTGATGATGVAGAIGPTGAVGATGPTGADGAVGPTGATGATGANGATGPTGAVGAT
                     GANGVAGPIGPTGPTGANGVAGAIGPTGPTGANGATGATGANGATGPTGAVGATGANG
                     VAGAIGPTGPTGANGATGATGATGATGANGATGPTGATGATGVLAANNAEFTVSSSSL
                     VNNTLVTFNSSFINGTNITFPTSSTINLAVGGIYNVSFGIRATLSLAGFMSITTNFNG
                     VAQNNFIAKAVNTLTSSDVSVSLSFLVDARAAAVTLSFTFGSGTTGTSAAGYVSVYRI
                     Q"
     gene            complement(3414..3953)
                     /gene="hpt"
                     /locus_tag="ACOIAF_003710"
     CDS             complement(3414..3953)
                     /gene="hpt"
                     /locus_tag="ACOIAF_003710"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HGZ2670688.1"
                     /translation="MDIETKKWEVLYSEEKIKDKLRELGAIIEKDYKDKNLMVVSLLK
                     GSFIFCADLVRNINLPLRVNFMTTSSYGNNEESTGRVKVVSDVTTDIAGYDVLVVDDI
                     TDSALTMDFVLKHLKAKNPASLKCCVLLDKPSRRKVDLVPDYCGFEIEDKFVVGYGFD
                     FGDYYRNVPYIFNVTDEDR"
BASE COUNT         1191 a          823 c          627 g         1540 t
ORIGIN      
        1 ttagttcatg gggcactctt tttggtataa agtcaaatat atttttatca tttatacaat
       61 gaaaaagaaa tttatctata ttttgaaaaa tccacttaaa ttttttaatg taagataaat
      121 tgtaagctta ttatcactaa tattatttta tataaattat attagtgata aaataaactt
      181 atagaaatat aacaaatttt tattaatttc ataggaggaa tttatgttga aagttataat
      241 tagtggctat aatggaacaa tgggaagagt tttagcagaa tgtgtaaaaa aagataatga
      301 attagagttg gtttgtggtg tagctaggtc tgctgatgaa aaaggtcatc atagtgatac
      361 aaaactttac aaaacaatga ctgatgtaac tgaaaaaact gatgtaataa tagacttttc
      421 acatcatgag actttagagc caatattgtc atatgctcta aaaaataata cacctgtagt
      481 attagctact actggatatg acgaaaatga cttaaataaa atagaagaag catcaaaaaa
      541 cattcctata ttattatcat caaatacatc tcttggagta aatatattaa taaaattagt
      601 taaggatgct tcaaagatgt tagaaggttt tgatattgaa atcatagaaa aacatcacaa
      661 tagaaaagaa gatgccccta gtggaactgc tactatgata gctaatgcaa ttagagaagt
      721 cttgcctgaa agtgaaaata cacatggtag atatggtaga gattgtaaaa gaaaaccaaa
      781 tgaaattgga atacactcta taagaggtgg caatataatc agcgaccatg atgttatatt
      841 tgcaggtgat aatgaagttc ttacattaag ccatcattct caatctgatg aagtatttgc
      901 aaacggttct atagttgcag ctaaattttt aattggtaag caatctggac tttataaaat
      961 gtcagatata ctaggataag ttttgtacat aataaatctc aatctattta ggtattcata
     1021 ttcatcaatt tgaatatcta attgattgag atttctattc taaaagttta taaattttat
     1081 gcttaccttt taaatataat catttttctt aaagattatc ttatttattg gtatatggaa
     1141 ttatattgtt tattatttta taatcttttc atcatataaa cctattaaat attatcctat
     1201 ttattggtat atgaaatcat attgtttatt attttatagt cttttcatca tataaaccta
     1261 ttaaatatta ttctatttat tggtatatga aatcatattg tttattattt tataatcttt
     1321 tcatcatata aactattgta ttctataaac tgatacatat ccagctgcag aagtacctgt
     1381 cgtgcctgaa ccaaatgtaa aacttaaagt aacagctgct gctctagcat caactaaaaa
     1441 gcttaaactt acacttacat ctgatgaagt aagtgtattt actgcttttg caataaagtt
     1501 attttgagct actccattaa agttagtagt aattgacata aatcctgcaa gtgaaagtgt
     1561 ggcacgtata ccgaaagata cattgtatat ccctccaact gcaagattta tagtactact
     1621 tgttggaaaa gttatattag ttccatttat aaatgatgaa ttaaatgtca ctaatgtatt
     1681 attcactaaa cttgaagaag acactgtaaa ttctgcattg tttgctgcta acactcctgt
     1741 tgctcctgtc gcccctgttg gacctgttgc tccatttgct cctgttgctc cggtcgcccc
     1801 tgttgctccg gtcgctccat ttgctccggt tgggcctgtt ggtcctatcg ctcctgctac
     1861 tccatttgct cccgttgctc ctactgctcc ggttgggcct gttgctccat ttgcccctgt
     1921 tgctccggtc gctccatttg ctccggttgg gcctgttggt cctatcgctc ctgctactcc
     1981 atttgctccg gttggaccgg ttggacctat tggacctgct actccattcg ctcccgttgc
     2041 tcctactgct cccgttgggc ctgttgctcc atttgcccct gttgctcctg tcgctcctgt
     2101 tggacctact gctccatctg ctcccgttgg gcctgttgct cctactgctc cggttggacc
     2161 tattgcccca gctactcctg ttgctcctgt cgctcctgtt ggacctacca aaccatttgc
     2221 tccggttgct ccggttgggc ctgttgggcc taccaaacca tttgctccgg ttgctccagt
     2281 tgggcctgtt gggcctgttg gacctaccaa accatctgga cctgttgctc ctactgctcc
     2341 tgttggacct acctctccat ctgctccggt tggacctgtt gctcctattg ggcctgttgc
     2401 cccagttgtt ccggttgctc cagtcgctcc tatacttccg gttggacctg ttgctcctgt
     2461 atttcctgtt ggtcctgtag cacctgttac tccatttgct ccggttgctc ctgtatttcc
     2521 tgttggacct gttattccat ttgctccggt tgctccggtt gctcctgtta gtccggttgg
     2581 acctgttgct ccagttgctc ctgtattccc tgttggacct gttattccat ttgctccagt
     2641 cgctcctgta tttcctgttg gtcctgtaga gcctgttatt ccatttgctc cagttgctcc
     2701 tttgtttcct gttggacctg ttattccatt tgctccggtt gctcctgtat tccctgtcgg
     2761 acctgttatt ccattcgctc cagtcgctcc tgtatttcct gttggtcctg tagagcctgt
     2821 tgttccattt ggtccagtcg ctcccatatt tcctgttgga cctgttattc cattcgctcc
     2881 ggttgctccc gtatttcctg ttggacctgt tattccattt gctccggtcg ctccagttgc
     2941 acctctaggc cccgtcggtc ctgttggacc agtagcacct gttggccctg ttggacctac
     3001 atcttgatat aaacttggac ctgaaatatc actcatataa ttaatcctcc ttttttaaag
     3061 ttagagtatt actctataaa tattgagtat tactctatat aattatatga atcgacaatt
     3121 tcttatgtga taatgtatct atttgcttat atcaacatat atgctttgat tcttgaattt
     3181 cattactttt ttgtaaaatc ttttacaatt attagtactc ttttacaatt attaatacaa
     3241 ttaaaaaact catttcccat caattattta aaattttttt attatgttat aaaaaataga
     3301 taattaaatt gcataaaatt aatttaatac tactttaatt actttgtaaa taaaaaaacc
     3361 tcaacacgat taataaaaat catattaagg catccacaaa attatataat tgcttatcta
     3421 tcttcatcag taacattgaa tatataagga acatttctat aataatcacc aaaatcaaac
     3481 ccatatccaa ccacaaactt atcttctatt tcaaatccac aataatctgg cactaaatcg
     3541 acttttcttc tgcttggttt atccaaaaga acacagcatt ttaaactagc aggattttta
     3601 gcttttagat gttttaacac aaaatccata gtaagtgcag aatcagtaat atcgtctaca
     3661 accaaaacat catatcctgc tatatcagta gtcacatctg aaactacttt aacccttcca
     3721 gtactttctt cattgtttcc ataactagat gtagtcataa aattaactct aagtggtaaa
     3781 tttatatttc taactaagtc tgcacaaaat ataaagctac cttttaatag agaaactacc
     3841 ataagatttt tatctttata atctttttct ataattgctc ctaattccct taatttatct
     3901 ttaatttttt cttctgaata caatacttcc catttttttg tttctatatc cataaaaaga
     3961 cctccaattg taataatcct tcttaatttt ataagagatt gctcactttt tcaatactct
     4021 tattcaatat tagtcaatat tattgagttt ttatgttcat tcaacaatta ttaacaatgt
     4081 atcttatatt ttaagtagtt catatatcca cactaaatta catactatat attttagaca
     4141 aaaaaagaaa aaacttgcac taacaagttt tttctattat a
//
LOCUS       DBGJFF010000215         4152 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.220, whole genome shotgun sequence.
ACCESSION   DBGJFF010000215 DBGJFF010000000
VERSION     DBGJFF010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4152
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.220"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(344..526)
                     /locus_tag="ACOIAF_003711"
     CDS             complement(344..526)
                     /locus_tag="ACOIAF_003711"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HGZ2670689.1"
                     /translation="MLNKKLPDTELKIMKCIWHTGYKTVISKDVADEIEKTYGWKHTT
                     TIILLARLTKKGFLIS"
     regulatory      752..849
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            904..1080
                     /locus_tag="ACOIAF_003712"
     CDS             904..1080
                     /locus_tag="ACOIAF_003712"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670690.1"
                     /translation="MFGNKMDKCTHVLTAYISSSYDYCNFLDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     gene            1306..1506
                     /locus_tag="ACOIAF_003713"
     CDS             1306..1506
                     /locus_tag="ACOIAF_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011723062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HGZ2670691.1"
                     /translation="MNNGIVKWFNNEKGFGFISMEGRDDVFAHFSAIQTSGFKSLEEG
                     QQVSFDIVKGARGPQAENITIL"
     gene            2322..2504
                     /locus_tag="ACOIAF_003714"
     CDS             2322..2504
                     /locus_tag="ACOIAF_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HGZ2670692.1"
                     /translation="MFKFYNNATLMFLPGTNIKTISERLGHADIKITMNSYSQFLEEM
                     DREAFENLSKILFIVF"
     gene            2575..3420
                     /locus_tag="ACOIAF_003715"
     CDS             2575..3420
                     /locus_tag="ACOIAF_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HGZ2670693.1"
                     /translation="MNIIKSITLLLTLLIVILMTGCSPRESASEVAKKYIDKMNSGDY
                     ETAYSLLSKDSKKKISIENLEEFQNILVSTKRIISYKIGKEKIIKKYKHDGKEYKNVV
                     KLEETFNIKNLLYKKDDSLKVNRYFVVENNKYKLLLYESFKKEFGINSMDLAQLKLND
                     FGLKYFKEVDLILKKAIAINPTDSKLYYAQACNYMNFDGFPGYSDTTYNALDSINKSL
                     KYLDKNDSDYKKNASNIYNLKGVILMFHKRFDEAQQYLNKALYFNKDNEDPKYNLNTI
                     KKHLN"
     gene            complement(3644..3808)
                     /locus_tag="ACOIAF_003716"
     CDS             complement(3644..3808)
                     /locus_tag="ACOIAF_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670694.1"
                     /translation="MFGKPKESVRFLDFNDVAEKFGEKKAVAIFDAIAGKKSKEHIIK
                     DIEKLVKNEV"
BASE COUNT         1583 a          523 c          479 g         1567 t
ORIGIN      
        1 aacatttatt tttttaactt ttgtcagtaa aatgtcagca aaacacttta tccacaattt
       61 ttatataagt tttccacaac ttatcaccaa tattatccac ataaaaaagg tagcacttat
      121 attattagct accttttatt tattaatctt tatatttcca gctttttatc caacttccta
      181 tttctattat tttttcttca gttatttctt ctccatcatg taattttgaa attaattctg
      241 ataaaggatt gttatgtaaa cctctaaaaa ctctttttcc ctctattttg agatattctc
      301 tctcttttat taatactgta tagtgtacat gcctcccaat tctctatgat attaaaaaac
      361 cttttttggt taatctagct aaaagtataa ttgtagttgt atgcttccaa ccatatgttt
      421 tttctatttc atctgcaaca tcttttgata ttacagtttt ataaccagta tgccatatgc
      481 atttcataat ttttaattct gtatctggta attttttatt tagcataata tattccttct
      541 ttcaatttat gtcacttctt caaatttatc tagcttaaat tataatagga gctttttaga
      601 acaaaaacta tatttaattt ttaccaggta ctctacgtgt ttagtctgtt tttaagtgta
      661 aaataatgtt catatctata aatttattta actttcaaac aatcctgttg aataataact
      721 aattctattg tattatttta gttgtagaat aaaactaaat cggcaaaact agagaaattt
      781 agtgacgcaa agctataggg actaagactt atataaatat cttatgagtt atgtcagcca
      841 gttgccaaag agatagtgtt ctttttgttt ttatgaaagt tttttagggg gataataatt
      901 tatatgtttg gaaataaaat ggataaatgt acacatgtat tgactgctta tattagcagt
      961 tcgtatgatt attgtaattt tttagataca cagttagatg attttatatt agagtacgga
     1021 gaaaatgtag tagagtcttg tttacaccaa gtaatggtat tggtaagtaa gtataattag
     1081 atagacattg atttctagaa aacccttttt agttttatct acatttatat taatatatag
     1141 caatgtaaaa cgtttaaata tatttttaat attaagaata attaaaatgt taactaaaaa
     1201 atataattaa gtagacattt ctattaatct atgatacaat agtatttgtg gtaatacaca
     1261 attaaatcaa gtatatcttg atataggtta ataggagtat ttttaatgaa taatggaata
     1321 gtgaaatggt ttaataatga aaaaggattc ggttttatat caatggaagg tagagatgat
     1381 gtgtttgctc atttctcagc tatacaaact tcaggattta aatcattaga agaaggtcaa
     1441 caagtaagct ttgatatagt taaaggtgct agaggtcctc aagcagaaaa tataactata
     1501 ttataatata tagatttttc gggaaagact ctttttagag tcttttttta ttgttaatat
     1561 atgtaagata atcattggtg aagtaaataa ggtatattga gtaatgttct tgatagttta
     1621 tatgctaatg aatttcaaaa aaataagcac tcctataaaa agagtgctct taatgtaaat
     1681 ataaaccttc attggtccag cctaataccc cgtattctcc aagttttaac tcttttttat
     1741 atacattatc actttcagcc atatctaaca caatcatttc ttctttagaa tcaatttgta
     1801 aaatatgagg actgtgtgtt gtgacaatta tttgtgtttt tgggagaatt gtttttaatg
     1861 taataaaaat tttacatacc acatagttga tataaaactt aagactagca gactcataag
     1921 caattagttg cctctcttta cataccacat agttgatata aaacctagag gctctcagag
     1981 aagttataag atgtttagta actttacata ccacatagtt gatataaaac ttaaaactag
     2041 atactaaact ggtgtaacaa tcgtttcttt acataccaca tagttgatat aaaactccaa
     2101 aataaactta gcatctccaa tgtttacaca tatacaatgc tcttaaaatt gcagtgaacg
     2161 cgcaatattg caattgataa tacttatcat atattctcaa taccttatat ttcaactgct
     2221 aaacctcaat ttatcacaaa tatcgctcac tgcaaaattt ctatattttt attatatcat
     2281 agcatataat taatattttc aattaatttt tacatttaat tatgtttaaa ttctataata
     2341 atgcaacttt aatgtttctt cctggaacta atataaaaac tatttcagaa agattaggtc
     2401 atgcagatat aaaaataact atgaatagct actctcagtt tttagaagag atggacagag
     2461 aagcatttga aaatttgagc aaaatactat ttattgtatt ttaaagaaga tgacttattc
     2521 aatatttgat gctagaatat aattaataaa aatttaatta taggtgattg tattatgaat
     2581 attattaaaa gcataacttt actcttaaca ttattaattg tgatattaat gacaggctgt
     2641 tccccaagag aaagtgctag tgaagttgca aaaaaatata ttgataagat gaactctggt
     2701 gactatgaaa cagcatatag tttattaagt aaagatagta aaaaaaaaat tagtatagaa
     2761 aatcttgaag aatttcaaaa tattttagtt agtacaaaaa gaataattag ttataaaata
     2821 ggtaaagaaa aaatcataaa aaaatataaa catgatggaa aagaatataa aaatgttgtt
     2881 aaattagaag aaacttttaa tataaaaaac cttctttata aaaaagatga ttctctaaaa
     2941 gtaaatagat attttgtagt tgaaaataac aaatacaagc tattattgta tgaaagtttc
     3001 aaaaaagaat ttggtattaa tagtatggac cttgctcaac ttaagcttaa tgattttgga
     3061 ctaaaatact ttaaagaagt tgaccttata ctaaaaaaag ctattgctat taatcctact
     3121 gattcaaagc tttattatgc ccaagcatgt aattatatga attttgatgg atttcctggt
     3181 tattcagata caacatataa tgctttagat tcaattaata aatctttaaa gtatttagat
     3241 aaaaatgatt ctgactacaa aaaaaatgca agtaatatat ataacttaaa aggagttatt
     3301 cttatgtttc acaaaagatt tgatgaagca caacaatatt taaataaagc cttatacttt
     3361 aacaaagata atgaagaccc aaaatataat ctaaatacaa taaaaaaaca tctaaactag
     3421 atatttaatt tttgaattta taaaatattt ttttatgtat aattcagcac tgaatcataa
     3481 tctctactaa actaattaca agtttaaatt ataaagactt atttctatat aaatattact
     3541 ctataacaat aagtttctta tttaaatttt aaaatattat ctaaaaatca taaaaaagaa
     3601 ggtataaaga gctaccctca agctcaatat accttataaa tatctaaact tcatttttta
     3661 ctaatttttc tatatctttg ataatatgtt ctttagattt ttttccagct atggcatcaa
     3721 aaattgcaac agcctttttc tctccaaact tttctgctac atcattaaag tctaaaaatc
     3781 taacactttc ttttggttta ccaaacatat aacacctctc atacattttt atatatttat
     3841 tataacttct atagacttat ttttcaccaa taaacgttag acaaattagt ccctttattt
     3901 ttttaaacta gctttacctt caaaattctt aacaaaaaca gtttattcta ctctataaat
     3961 aaaaatagac aatttaattt gtaggaaact gataatagaa tacagtaaat aaaactttgt
     4021 ttaatctatc tgaaaaagtt atctattaaa actgctatga atttataaag aatattctag
     4081 ttcataaaaa tacaaatatg taaatataat caccttaata ttcaatttat ttaaaataaa
     4141 aaatgtataa aa
//
LOCUS       DBGJFF010000216         4148 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.221, whole genome shotgun sequence.
ACCESSION   DBGJFF010000216 DBGJFF010000000
VERSION     DBGJFF010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4148
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.221"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            72..1670
                     /locus_tag="ACOIAF_003717"
     CDS             72..1670
                     /locus_tag="ACOIAF_003717"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HGZ2670695.1"
                     /translation="MSLLVFGHKNPDTDSICSAISLAHLKNKLGIKATAYALGDIRKE
                     AKYALDYFKVDAPEILDNVRIQVRDLNYDKVVPLTPTSSILEAYNLMDEKNVKTLPVT
                     YDDGTFAGIITMKEIAKNLLHQHFTTIHTSLSNICKNLNGTILVDCGEDIKGRVVTLS
                     FGMETLIETLKEGDIAIVGDRYDSIEYAIDTKVKLLILTNHTEISKDLLALAKINGVS
                     VVSVESDNYKTSNVINQCSFLDSIEATENLITFKEDDYMDEIKEIMLETNFRSYPILD
                     DDNKFLGLVSKGHLLNPSKKNVVLVDHNEYAQSADGIEQANIVEIIDHHKLGGISTDV
                     PMSFRVMPVGCNSTIIYQMYKENNVEIPYEIAGLLLSAILSDTLLFKSPTTTDIDKKA
                     CEELSKIAKVDMEKYAMDMFKCGTSLDEYTIEEIVNMDFKEFNMSGHRVGIGQVFTLD
                     IDSIFAKKDEFLSYINSTDYDKLILSVTDIIKEGSYLIYKAEDSLIANAFGVEGVQGT
                     FAPGVVSRKKQLVPNLTTAIKNFK"
     gene            complement(1905..3842)
                     /gene="bclA1"
                     /locus_tag="ACOIAF_003718"
     CDS             complement(1905..3842)
                     /gene="bclA1"
                     /locus_tag="ACOIAF_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA1"
                     /protein_id="HGZ2670696.1"
                     /translation="MRNIILYLNDDTFISKKYPDKNFSNLDYCLIGSKCSNSFVKEKL
                     ITFFKVRIPDILKDKSILKAELFIHIDSNKNHIFKEKVDIEIKRISEYYNLRTITWND
                     RVSMENIRGYLPVGISDTSNYICLNITGTIKAWAMNKYPNYGLALSLNYPYQILEFTS
                     SRGCNKPYILVTFEDRIIDNCYPKCECLPIRITGPMGPRGATGSTGPTGVTGPTGSTG
                     ATGSIGPTGPTGNTGATGSIGPTGVTGPTGSTGATGSIGPTGVTGPTGNTGVTGNTGV
                     TGNTGVTGNTGVTGSIGPTGATGPTGVTGEIGPTGVTGPTGVTGEIGPTGATGATGVT
                     GSIGPTGATGPTGATGVTGEIGPTGEIGPTGATGPTGVTGSIGPTGATGPTGEIGPTG
                     ATGSTGVTGSIGPTGATGPTGATGEIGPTGATGPTGNTGVTGEIGPTGATGPTGNTGV
                     TGEIGLTGATGPTGNTGATGSIGPTGVTGPTGATGVTGPTGPTGATGNSSQPVANFLV
                     NAPSPQTLNNGDAITGWQTIIGNSSSITVDANGTFTVQENGVYYISVSVALQPGSSSI
                     NQYSFAILFPILGGKDLAGLTTEPGGGGVLSGYFAGFLFGGTTFTINNFSSTTVGIRN
                     GQSAGTAATLTIFRIADTVMT"
BASE COUNT         1245 a          742 c          632 g         1529 t
ORIGIN      
        1 atactcacta ccttttgata taatattttt atcaattatt attatattca agttttgaaa
       61 ggagtattaa aatgagttta ttagttttcg gacataaaaa tccagataca gattcaatat
      121 gttctgcaat ttcattagca catttaaaga acaaactagg aataaaagca acagcttatg
      181 ctttaggaga tatcagaaaa gaagctaaat atgccttgga ttactttaag gtagatgcac
      241 ctgaaatttt agataatgtt agaatacaag ttagagattt aaattatgat aaagtagtac
      301 ctttaacacc aacttcatct atattagagg cctataacct tatggatgaa aaaaatgtta
      361 aaacactacc tgtaacttat gatgatggaa cttttgctgg tatcattact atgaaagaaa
      421 tagctaaaaa tctacttcat caacacttta caactataca cacatcacta tcaaacattt
      481 gtaaaaacct taatggtact attcttgtgg actgtggtga agatataaaa ggtagagtag
      541 tcactttatc atttggaatg gagactctaa tagagacttt aaaagaagga gacatagcta
      601 ttgttggaga tagatatgat agtattgaat atgctattga tacaaaagtt aaacttttaa
      661 tacttactaa tcatacagag atttcaaaag atttgctagc tcttgctaag ataaatggag
      721 tgtctgttgt ttctgttgaa agtgacaatt ataaaacttc taatgtaata aatcaatgta
      781 gtttcttaga ctctatagaa gctacagaaa atttaattac attcaaagaa gatgattata
      841 tggatgaaat aaaagagatt atgctagaaa caaacttcag atcttatcca atattagatg
      901 atgataataa gtttttaggt ttagtttcaa aaggtcatct attaaaccca tctaaaaaga
      961 atgttgtttt agttgaccat aacgaatatg ctcaaagtgc tgatggaatt gaacaagcaa
     1021 acatagttga aataattgac catcataaac ttggtggtat ttctactgat gttcctatgt
     1081 catttagagt tatgcctgtt ggatgcaata gtactataat ttatcaaatg tacaaagaaa
     1141 ataatgttga gataccttat gaaatagccg ggcttctttt atctgctata ttatcagata
     1201 cattattatt taagtctcca acaacaactg atatagataa gaaagcttgt gaagaattaa
     1261 gtaaaatagc aaaagtagac atggaaaaat acgctatgga tatgtttaaa tgcggtactt
     1321 ctttagatga atatactata gaagaaatag taaatatgga ctttaaagaa tttaatatga
     1381 gtggacatag agttggtatt ggacaagtat ttaccttaga catagactct atatttgcta
     1441 aaaaagatga gtttttatct tatataaatt cgactgatta tgataaatta attctttcag
     1501 ttacagatat aatcaaagaa ggttcttatt taatctataa agcagaagat agcttaattg
     1561 ctaacgcatt tggtgttgaa ggtgttcaag gcacatttgc accaggtgtt gtatcaagaa
     1621 aaaaacaatt agtccctaat ttaactactg caatcaaaaa ttttaaataa tataaaattt
     1681 ttaaataacg aaaactgtct caaatctata tttgagacag ttttttatta tatatatatt
     1741 gataataaag ttaaataaaa atccataaat ttaaaatttt tatgaatata ttttattatt
     1801 attgttattt tattcattct aaaataggta aaactcaata tataaaaata cttttgtcat
     1861 ttaaataaag cctaaatagt ttttaagatt attttagaca cgttttaagt cataacagta
     1921 tcagctattc taaatatcgt caaagtagcc gcagttcctg ctgattgccc atttcgtatc
     1981 cctactgttg tagatgaaaa attatttatt gtaaaagtag tcccaccaaa taaaaaacca
     2041 gcaaaatatc cagaaagtac tcctccgcct cctggctcag tagtaagccc tgccaaatct
     2101 tttcctccta aaattgggaa taggatagcg aaagaatatt gatttatact tgatgaacct
     2161 ggttgtaatg ctactgaaac tgatatataa tacacaccat tttcttgtac tgtaaacgta
     2221 ccatttgcat ctactgttat acttgaacta tttcctatta ttgtttgcca acctgttata
     2281 gcatctccat tatttagtgt ttgtggagat ggtgcattta cgaggaagtt agcaactggc
     2341 tgagaggaat tgcctgttgc tcctgttgga cctgttgggc ctgttactcc tgtcgctcct
     2401 gttggtcctg ttactcccgt tggccctata cttcctgtcg ctcctgtatt tcctgttggg
     2461 cctgttgctc ccgttagtcc tatttctcct gtcactcctg tatttcctgt cggacccgtt
     2521 gctcccgttg gtcctatttc tcctgttact cctgtatttc ctgttgggcc tgttgctccc
     2581 gttggtccta tttctcctgt cgctcctgtt gggcctgttg ctcccgttgg tcctatactt
     2641 cctgtcactc ctgttgagcc tgttgctcct gttgggccta tttctcctgt tgggcctgtt
     2701 gctcccgttg gtcctatact tcctgtcact cctgttgggc ctgttgctcc cgttggtcct
     2761 atttctcctg ttggccctat ttctcctgtc actcctgtcg ctcctgttgg acctgttgct
     2821 cctgttggtc ctatacttcc tgtcactcct gtcgctcctg ttgctcccgt tggtcctatt
     2881 tctcctgtca ctcctgttgg gcctgttact cccgttggtc ctatttctcc tgtcactcct
     2941 gttgggcctg ttgctcccgt tggtcctata cttcctgtca ctcccgtatt tcctgtcact
     3001 cccgtatttc ctgtcactcc cgtatttcct gtcactcccg tatttcctgt cggacctgtt
     3061 actcctgttg gtcctatact tcctgtcgct cctgtacttc cggttgggcc tgttactccc
     3121 gttggcccta tacttcctgt tgctcctgta tttccggttg ggcctgttgg tcctatgctt
     3181 cctgtcgctc ctgtacttcc ggttgggcct gttactcccg ttggtcctgt acttcctgtc
     3241 gctcctcttg gtcccattgg acctgtaatt ctaattggaa gacactcaca tttaggataa
     3301 caattatcta taattctatc ttcaaatgtt acaagtatat acggtttgtt acaacctcta
     3361 ctagatgtaa attcaagaat ctgataaggg taatttaaag ataaagctaa cccataatta
     3421 ggatatttat tcattgccca tgcttttata gttcctgtaa tatttaaaca aatatagttg
     3481 gatgtatcac ttatcccaac tggtaaatat cccctaatat tttccataga cactctatca
     3541 ttccatgtta tagttcgtaa attataatat tcacttattc ttttaatttc aatatctact
     3601 ttttctttaa aaatatgatt cttatttgaa tcaatatgaa taaataactc tgcttttaat
     3661 atacttttgt cttttaatat atctggtatt ctcactttaa aaaaagtaat caacttttct
     3721 tttacaaaac tatttgaaca tttacttcct attaagcaat aatctaaatt actaaagttt
     3781 ttatctggat attttttaga tataaaagta tcatcattta aataaagtat aatatttctc
     3841 atttataatc cctccatttt gggtctatat catactatgt ttaaaataca tgcttagtta
     3901 caatataatt aaaatttata gtattaaatt tttatataat aatgctgatt tctacaaaaa
     3961 aagaacatgt cattagaaat ttctaatagc atgttctttt tcattattat atattacact
     4021 ttaaattatt ccttgactgg cttagctggt gtaaactaaa ccagtctttc taaaatatta
     4081 agcacttttt tcttattatg ttttttagga tttattgtct atattattgt aaaccaaagt
     4141 tataaaat
//
LOCUS       DBGJFF010000217         4137 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.222, whole genome shotgun sequence.
ACCESSION   DBGJFF010000217 DBGJFF010000000
VERSION     DBGJFF010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4137
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.222"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            163..561
                     /locus_tag="ACOIAF_003719"
     CDS             163..561
                     /locus_tag="ACOIAF_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGZ2670697.1"
                     /translation="MIKKIEHVVITNNNIENYINFYKKFGFSSKEMSKNYKLNTRNVK
                     INLNIKGYKFLPYVKNLQASRDNLCFKINTNIDEPKKYLEQNEIQIERGIVECTGVFG
                     NMKSSYLKKTNGNLIELSNHRITESKPWKI"
     gene            549..1487
                     /locus_tag="ACOIAF_003720"
     CDS             549..1487
                     /locus_tag="ACOIAF_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HGZ2670698.1"
                     /translation="MENLILSINVVLPLFLTMSLGYILKKLGLFDTHTLNKMNNVCFK
                     SFLPLLLFFNIYNTDLDGSLNFKLMAVAVIIIFSIFLILVFIIPKIEKDNKTRGALIQ
                     GVFRSNFVIFGIPVTISLFGDKSIGVTSLLIAVIVPMFNVLSVVILEIFRGGKVNIKH
                     MIKSVLTNPLIIASFIGLTMLILNINIPTFLHKTIGDISKIATPLSLIILGGSFSFSA
                     IKGHIKPIILGVCGKLILVPAIFLPISIFLGFRNVELASLMIMLSAPTAVSSFTMAQQ
                     MDSDSELAGQIVVFTSGFSIITVFLIIFILKQNNLI"
     gene            complement(1871..2095)
                     /gene="secG"
                     /locus_tag="ACOIAF_003721"
     CDS             complement(1871..2095)
                     /gene="secG"
                     /locus_tag="ACOIAF_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HGZ2670699.1"
                     /translation="MSNILMGVQVVLGILLVISIMPQDSKNALPSEFGGEGSQAYFKP
                     KGKQAFLARVTKITAVLFFINALALLIVKK"
     gene            complement(2700..3992)
                     /gene="eno"
                     /locus_tag="ACOIAF_003722"
     CDS             complement(2700..3992)
                     /gene="eno"
                     /locus_tag="ACOIAF_003722"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HGZ2670700.1"
                     /translation="MSVIELVYAREVLDSRGNPTVEVEVVLEDGAMGRAIVPSGASTG
                     AFEAVELRDGDKGRYLGKGVETAVANVNEIIAPEIEGMDAFDQPAIDAIMIELDGTPN
                     KGKLGANAILGVSMAVARAAADEIGLPLFQYLGGVNAKQLPVPMMNILNGGEHADNNV
                     DVQEFMILPVGACCFKEGLRMGAEVFHSLKKVLGEKGLACGVGDEGGFAPNLGSNREA
                     LELIVEAITKAGYKPGEDVMLGLDVAATEMYNKETKKYVLAGEGKELTAAEMVALYED
                     WSNNFPIITIEDGLDEEDWDGWKLLTEKLGNKLQLVGDDLFVTNTERLEKGIENGVAN
                     SILVKVNQIGTITETLDAIEMAKRAGYTAVISHRSGETEDSTIADLAVAVNAGQIKTG
                     APSRTDRVAKYNQLLRIEEMVGEQARYCGLKSFYNLKK"
BASE COUNT         1437 a          659 c          462 g         1579 t
ORIGIN      
        1 aactctacat aaagttttat aatctaaatg aaatacaata atctgcataa aattcaatag
       61 cttatataaa atttagtaat ttatataaat attatcaacc tatataaaat ttaataactt
      121 atataaattt aactaattta tataaaatct taaggagttg ccatgataaa aaaaatagaa
      181 catgtggtca ttactaataa taatatagaa aattatatca atttttataa aaaatttggt
      241 ttttcatcta aagaaatgag caaaaattat aaacttaata ctagaaatgt taagataaat
      301 cttaacataa aaggttataa atttttacct tatgttaaaa atttacaagc aagtagagat
      361 aacttatgtt ttaaaataaa cacaaatatt gatgagccaa aaaaatactt agaacaaaat
      421 gagattcaaa ttgagcgagg aattgtagaa tgtacaggtg tctttggtaa tatgaaatct
      481 agttatttga aaaaaactaa tggaaattta attgaattat caaatcatag aatcacggag
      541 agtaaacctt ggaaaattta atcctatcaa taaatgttgt tcttcctttg tttctcacaa
      601 tgtcacttgg atatattcta aaaaaattag gtctatttga tactcatact ttaaataaaa
      661 tgaataatgt ctgcttcaag tctttcttgc cattattatt attttttaat atatacaata
      721 cagatttaga tggttctctt aattttaagc ttatggctgt agctgttata attatttttt
      781 caatcttctt aatcttagtc tttattattc ctaaaattga aaaagataat aagacaaggg
      841 gtgcattaat acaaggtgta tttcgtagca atttcgttat atttggtatt cctgtgacaa
      901 tttcattatt tggcgataag agtattggag tcacatcttt attaattgca gttattgtac
      961 ctatgtttaa tgtattatct gttgtaattc ttgaaatttt tagaggtggc aaagtaaata
     1021 taaaacatat gattaaaagc gtcttaacaa atccactcat aatcgcctct tttataggat
     1081 tgacaatgct aattttaaat attaatatac ctacattttt acacaaaact attggtgata
     1141 tttctaaaat agctactcct ttatcactta taattctagg cggctctttt agtttttctg
     1201 ctataaaggg gcatataaaa cccattatat tgggcgtatg tggaaaactt atacttgtac
     1261 ctgctatatt tttaccaatt agcatttttc ttggatttag aaatgtagaa ttagcttcac
     1321 ttatgataat gttatctgct ccaactgcag tttcttcatt tacaatggca caacagatgg
     1381 attctgacag tgaattagct ggacaaattg tagtatttac ttctggattt tctataatta
     1441 cagtattttt aataatattt attttaaagc agaataatct tatttaatga tatttgataa
     1501 cacaaattgg tattatgaga aacatatata aaattgattt aattgcgtaa aaacatatta
     1561 ttatactatt tttactatat aatataactt tgtgtatatt atctcattaa acatggtgaa
     1621 agtagtgttg ctatatattt tttagtttga agaaaatata taaatacaaa caatatataa
     1681 atacaaataa taaacgccaa caaatagtca atctataaaa taaaaactgg actaatcata
     1741 taaaatcatt agtccagctt ttattttacg tactttttat gttgatgtac ttttattaaa
     1801 aactaaattt aacttttaaa tgtctagcaa atgcactaat taaataatgg ccagttattc
     1861 aacaatatca ctacttctta actataagta atgccaatgc atttataaaa aataatactg
     1921 ctgttatctt tgtaactcta gcaagaaatg cttgttttcc ttttggttta aagtaagctt
     1981 gacttccttc tccaccaaat tctgaaggta aagcattctt agaatcttga ggcattatgc
     2041 taattactag taaaatccct agtactactt gcacacccat taaaatgttg ctcattcttg
     2101 cccctcctat ctgtttatta cttaaatttc attactcttt agtgtatcac aataatatag
     2161 ccatttcaag actattatgc tataaataac atacaaaaaa atatattgtt actataatat
     2221 attactatat tctcaatata aaaatgaata aaacttagaa gagaaaacta tttaaattaa
     2281 tacataaatt aatgcataaa ttaacactta aaataatcta ctctcattgc atatattata
     2341 aatagccacc aaaataaaac aatcatttta aaatattgat ataactttta atttcaacat
     2401 aatttaaaat aaataatcca catttagcaa taatacttta atgacaatta tgattaaatc
     2461 aaaatatgtc ttcactttta ttttttaata aaaacctcct atgcaattag ataaaaatga
     2521 gatagaaaca tttattcata ttttttctga atctatgaat aaaattaagt aataaaatat
     2581 aaaaaaggat aagtgaattg aaaaatcaat taacacttat ctcttttata tcttataaga
     2641 tgagcttaaa tatcttatat tctctcttca ttaatctttt ttagtaacca ttttaataac
     2701 tattttttta agttatagaa agattttaat ccacaatatc tagcttgctc tccaaccatt
     2761 tcttctattc ttaataattg gttgtactta gcaactctat cagttcttga tggagcacca
     2821 gtttttattt gaccagcatt tactgcaaca gctaaatctg ctatagtaga atcttcagtt
     2881 tctcctgatc tgtgagatat aacagcagta tatcctgctc tcttagccat ttctatagca
     2941 tctaaagttt cagttattgt acctatttga ttaactttaa ctaatataga gttagctact
     3001 ccattttcaa ttcctttttc taatctctct gtgttagtaa caaataagtc gtcaccaact
     3061 aattgtaatt tatttcctaa tttttcagtt aataatttcc atccatccca atcttcttcg
     3121 tctaaaccat cttctatagt tattattggg aagttgtttg accaatcttc atataaagca
     3181 accatttcag cagccgttaa ctcttttcct tctcctgcta aaacgtattt tttagtttct
     3241 ttgttgtaca tttctgtagc agcaacgtca agacctaaca taacgtcttc acctggctta
     3301 tatccagctt ttgttatagc ttcaactatt aattctaaag cttctctgtt tgaacctaag
     3361 tttggagcaa atccaccttc gtcacctaca ccacaagcta atcctttttc tcctaaaact
     3421 ttctttaatg aatggaatac ttctgctccc attcttaaac cttctttgaa acaacaagct
     3481 cctactggta atatcataaa ttcttgaaca tcaacgttgt tatcagcatg ctctccaccg
     3541 tttaatatgt tcatcattgg aacaggtaat tgtttagcat ttactccacc taagtattgg
     3601 aataatggta aacctatttc atcagcagct gctctagcta ctgccattga aacacctaat
     3661 atagcattag ctcctaattt acctttgttt ggagttccat ctaattctat catgattgca
     3721 tctatagctg gttggtcaaa tgcatccatt ccttctattt caggtgctat tatttcattt
     3781 acgttagcaa cagctgtttc tacacctttt cctaagtatc ttcccttatc tccatctctt
     3841 aattcaacag cttcaaaagc tcctgtagat gctcctgatg gaactattgc tcttcccatt
     3901 gctccatctt ctaatactac ttcaacttct acagttggat ttcctcttga gtctaatact
     3961 tctctagcat atactaattc aataactgac atttgtatat ctcccttcga ttttctatta
     4021 gttaaatttt catttactgt aaacatccgt ctactatttt aatctacttt ctatattata
     4081 gtacataaag ttgcttttga aaattaggta caaatttatt tgatgggtaa atttgta
//
LOCUS       DBGJFF010000218         4076 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.223, whole genome shotgun sequence.
ACCESSION   DBGJFF010000218 DBGJFF010000000
VERSION     DBGJFF010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4076
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.223"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            178..1068
                     /locus_tag="ACOIAF_003723"
     CDS             178..1068
                     /locus_tag="ACOIAF_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HGZ2670701.1"
                     /translation="MSSTIKSNRIKGIMFIIASALGFAMMSAFVKLAGDLPSFQKVFF
                     RNLVSAIIAFWLIIKHKGSLTGKKENLKVLLYRSIFGTLGVIFNYYAIDKLMLSDANM
                     LNKMSPFLVVIFCAIALKEKINVKQIGAIIIAFIGALFIIKPTFSVEVIPYLGGIAGA
                     VFAAMAYTCLRVLGDREDYYTIVFFFSVFSLVTVGPIAFAVYEPMTLMQLIYLLLGGV
                     FASLGQFGITLAYKYAPAKEISIFDYSNIIFSAILSIFLINVYPDMLSVVGYLIVFSA
                     AFYMFLYNKKLDKLDKEKKK"
     gene            1286..2572
                     /locus_tag="ACOIAF_003724"
     CDS             1286..2572
                     /locus_tag="ACOIAF_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HGZ2670702.1"
                     /translation="MEKVKVAVIGAGNRGTYAYAPYIYENSDVCEIVAVAEPKKGRRE
                     LFTQKYNLDSKNVFETLEDFFKHDKMADAVIIATNDDRHYDVAKLALEKGYHVLLEKP
                     MSNSLDGLVHIDDLCDKYKDKIFMICHVLRYSPFYNKLKEIVESKKLGELVSIQYNEN
                     IGYWHFAHSFTRGNWRNSNETSPLILAKSCHDMDILLYLVGSRCKKISAFGSLKHLNN
                     QNASGEMAQNCLQCLVEKKCPYSAKRIYLEKDRSINRAVHINPTEENLLNILKTSPYG
                     RCVYRCDNNVVDNMVNILQFENGVTATFNLCAFTKENGRTIKLMFSHGEVGGDLNKNE
                     IRIKEFGKNEEIVMNPSNQQNMVEEYDRNLIAEFIKLVSNKELEKGRVAAKEAIQSHV
                     MAFAAEYSRVSDEVVYIEEFFDSAKQMTKEIEETIF"
     gene            complement(2661..3104)
                     /locus_tag="ACOIAF_003725"
     CDS             complement(2661..3104)
                     /locus_tag="ACOIAF_003725"
                     /inference="COORDINATES: protein motif:HMM:NF025547.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory YrvL family protein"
                     /protein_id="HGZ2670703.1"
                     /translation="MIKISNKRRIPLGEFISVFLFFAVFFGITFCIFTVIGIGFLSFL
                     GFEYKSLGAVLIFFLIYFCITTPIDFLCTTILDIFRYVNKLPYSIYKLCEFIIDFILT
                     FLAMNIIDTFMDSVTIPLSTEILFALLSYLLSECMDFFDRDKKKP"
     gene            complement(3128..3922)
                     /locus_tag="ACOIAF_003726"
     CDS             complement(3128..3922)
                     /locus_tag="ACOIAF_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HGZ2670704.1"
                     /translation="MKLYLSADIEGTCGIVNWDETKLECEFSQHYRTQMSKEITAACD
                     GATQSGVHEILVKDAHDSGRNINPNILPENVKILRGWTKDPLVMMAGIDESFDACIFT
                     GYHSGATCNGNPLSHTMDIDYDYFKINDEIASEFTINAYIAAYYNVPVAFLSGDEMLC
                     ESAKKLNPNIVTVPVSKGIGNGSISIHPNLALKKIEKGVKKALSGDLSRHLIKLPDKF
                     KIEIKFREHYKAFKASFYPNMKKIDSQTVLFETEDYYEFLRMLLFI"
BASE COUNT         1574 a          477 c          636 g         1389 t
ORIGIN      
        1 ataacttttt agctataatt attattgtta ttaattgaaa agatttatta gttacttgac
       61 atagtttaca cttttaatta tagtataaat tggaatattt agataattat tatttaatgt
      121 ataagctaag tttttaatta atatggcttt atacatttat atagaaaaga ggtacgtatg
      181 agtagcacaa tcaaatcaaa tcgaataaaa ggtataatgt ttataatagc atcagcgctt
      241 ggctttgcaa tgatgtctgc attcgtaaag ctagctggag atttaccaag ttttcaaaaa
      301 gtgttcttta gaaatctggt atcagctata atagcttttt ggcttatcat aaaacataaa
      361 ggaagtctaa ctggtaaaaa agaaaattta aaagtacttt tatataggtc aatatttgga
      421 actctaggag ttatatttaa ctactatgca attgacaagt tgatgctttc agatgctaat
      481 atgctaaata aaatgagtcc cttccttgta gtaatatttt gtgcaattgc tctaaaagaa
      541 aaaataaatg taaagcagat aggagctatt ataattgcat ttataggagc tttattcata
      601 ataaaaccaa catttagtgt agaagtaatt ccatatcttg gaggaattgc aggagctgta
      661 tttgcagcaa tggcatatac ttgtttaaga gttcttggag atagagaaga ttactataca
      721 attgtatttt tcttttcagt tttttcatta gttactgtag ggccaattgc ttttgcagta
      781 tatgaaccaa tgacccttat gcaacttata tatctattac taggtggagt atttgcaagt
      841 ctaggtcaat ttggaataac tctagcatat aaatatgcac ctgctaagga aatctctata
      901 tttgactata gtaatataat cttttcagca attttaagta tattcttaat caatgtctat
      961 ccagatatgt taagtgttgt tggatattta atagtatttt cggcagcatt ttatatgttt
     1021 ttatacaata agaagttaga taaattagac aaagaaaaga agaagtaaac tagataaaag
     1081 tgtttttata atttgtattt aatatttaag tctttgtatt aaaattaaag aaaatttaat
     1141 taaaaatgtt tggtatatta acttataaca gcagttaaaa ttgaaaaatt ataactgctg
     1201 tttcttttat taaaaatatc caaatgtctg taaaaataga ctataataga gtagtataaa
     1261 taatataaag gggataaatt aaagtatgga aaaagtgaaa gttgctgtga ttggagcagg
     1321 taatagagga acgtatgcat atgctccata tatttatgaa aattctgatg tatgtgaaat
     1381 agttgctgta gcagaaccta aaaaaggaag acgagaacta tttacacaaa aatataacct
     1441 agatagtaaa aatgtatttg aaactttaga agactttttt aaacatgaca agatggcaga
     1501 tgcagtaata atagctacaa atgatgatag acattatgat gtagcaaaac tagctcttga
     1561 aaaaggttat catgtactat tagaaaagcc aatgtctaat agtttagatg gtttggtaca
     1621 tatagatgac ttgtgtgata agtataaaga taagattttt atgatttgcc atgtactgag
     1681 atattctcca ttttataata aattaaaaga aattgtagaa agcaaaaaat taggagagct
     1741 agttagtata caatataatg aaaatatagg atattggcat tttgcacata gctttactag
     1801 agggaattgg agaaatagca atgaaaccag ccctttaatt ttagcaaaga gttgtcatga
     1861 catggatatt ttgttatatt tagtaggaag tagatgcaaa aaaatatctg catttggaag
     1921 cttaaagcat cttaataacc aaaatgctag tggagagatg gcacaaaatt gtcttcagtg
     1981 tttagttgaa aaaaaatgtc catattcagc aaagagaatt tatttagaaa aagatagaag
     2041 tataaataga gctgttcaca taaatccaac tgaagagaat ctattaaata tacttaagac
     2101 aagtccatat ggaagatgtg tttacagatg tgataataat gttgtagaca atatggtaaa
     2161 tatactacag tttgaaaatg gagtgacggc tacttttaat ttatgtgcat ttactaaaga
     2221 aaatggaaga actataaagt taatgtttag ccatggtgaa gttggtgggg atttaaataa
     2281 aaatgagata agaattaaag aatttggaaa aaacgaagaa atagttatga atccatcaaa
     2341 tcaacagaat atggtagagg aatatgatag aaatcttata gctgaattta ttaaattagt
     2401 ttcaaataaa gaactagaaa agggtagagt agcagcaaaa gaagctatac aaagtcatgt
     2461 aatggcattt gcagcagaat attctagggt cagtgatgaa gtagtgtata tagaagaatt
     2521 ttttgatagt gctaagcaaa tgactaagga gatagaagaa actatatttt aattaataaa
     2581 aatttattaa ataaaataaa gatagattaa ataatagaaa tacgaaaata tgtgttctat
     2641 taaggaatct atctttttta ttaaggtttt ttcttatccc tatcaaaaaa atccatacat
     2701 tctgataaaa gataagaaag taaagcgaat aagatttctg tacttaaagg aatcgttact
     2761 gaatccataa aggtatcaat tatgttcata gctagaaaag ttagtataaa atcaattata
     2821 aattcgcaaa gtttatatat tgagtatggt aatttgttta catatctgaa tatatctaaa
     2881 atagtagtgc atagaaaatc aattggagtg gttatacaaa aatatattaa aaagaaaatt
     2941 aatacagctc caagagattt atattcaaat cctaagaatg ataagaagcc aattcctata
     3001 accgtgaaaa tacaaaaagt tataccaaaa aaaacagcaa aaaataaaaa tacagaaata
     3061 aattcgccta aaggaatcct tcttttatta cttatcttaa tcaactcctt aatttatgat
     3121 atgaaattta tataaataat aacattctta aaaattcata atagtcctct gtttcaaata
     3181 gtactgtttg agaatctatt tttttcatat ttggataaaa agaggcctta aaagctttat
     3241 aatgttctct aaattttatt tctatcttaa atttatctgg caattttatt aagtgcctag
     3301 ataaatctcc agataaagct tttttgacac ctttttcaat tttttttagt gcaaggtttg
     3361 gatggattga aattgaacca tttccaattc cttttgacac aggaacagtg actatatttg
     3421 gatttagttt ttttgcactc tcacaaagca tttcatctcc acttaaaaat gctacaggaa
     3481 cattataata tgcagcaata taagcattta tagtaaattc agatgctatt tcatcattta
     3541 ttttaaaata gtcataatca atatccatag tatgggaaag tggattacca ttacaagtag
     3601 ctccagagtg atagccagtg aatatacatg catcaaagct ttcatcaatt ccagccatca
     3661 taactagtgg gtctttggtc catcctctaa gtattttgac gttttcaggt aaaatatttg
     3721 gattaatatt tctaccactg tcatgtgcat ctttaacaag tatttcatgt acaccagatt
     3781 gagtagctcc atcacatgct gctgttatct ctttactcat ttgtgttcta tagtgttgcg
     3841 aaaattcaca ttcaagtttt gtttcatccc aatttacaat accacaagta ccttctatat
     3901 cagcacttaa atacaatttc ataatatata cccccttatg tttattattg atgataataa
     3961 cagataacag aaaatacata atcaataaag ggcataaaaa tattatgtct tactattaac
     4021 tattattaaa tctgtaactg aaaaataact aaattggtat aaaaaaatat aacact
//
LOCUS       DBGJFF010000219         4021 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.224, whole genome shotgun sequence.
ACCESSION   DBGJFF010000219 DBGJFF010000000
VERSION     DBGJFF010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4021
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.224"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            198..1703
                     /locus_tag="ACOIAF_003727"
     CDS             198..1703
                     /locus_tag="ACOIAF_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HGZ2670705.1"
                     /translation="MKIQNTEWGYIEWKHTYDENNPKQAMNIYIAVTMPGKKHFNHVH
                     YGQEQMIYILEGEGLYIINGVWKPFYQGMIFYIESGSTHETINTGDREIKELIVSNNV
                     DDVGESEVIDINPNNYLKKTLINYSESTLNLYAAVESIRGQFIDPFKIPLIIYDDSWN
                     IVLKNPYFPLFCFEKCNPMKFPQNCDCMNQKSSNQFVCEYGITIYNIPILYKNNSIGV
                     IRGGYVLLSDLNLDTEHNNLYDIPEGAARSIKRLLKQISKNIINFCSFNDIRKDLQEK
                     EKTIARTYHYGEQLEMNLKVAQDMVTNLRINHHFLFNTLNSMASIALDDGSYDLYSAI
                     IDLSRMFRYTMRSDLRFVELESEILYIKNYLNLQKLRYGDALKVKYLIPEKLYNLSVP
                     FNFIQPIVENAFTHGFRDIDTEKRIEIIARLDSQYAIIEIHNNGTILDGNNIDKIKAG
                     IRSNNGHGLSLIYTKFTSAYGNNFDMDIKSSDNEGTYIMIKIPIENYKEYV"
     gene            1817..2515
                     /locus_tag="ACOIAF_003728"
     CDS             1817..2515
                     /locus_tag="ACOIAF_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HGZ2670706.1"
                     /translation="MRRALIIDDEEAVLKIITHFIEMKDMPIKIVGKATSGDEAVDKI
                     IGLEPDIVFIDIQMPIYNGLEVIEKTSHLSKKINFVVITAFNYFEYAQKALRLNVKDI
                     LLKPLDMKEFTKSVEKIIGYQYTNNDLLNEILEYINLNYSNDLKLNDCARLFLTNPSN
                     ISRIFKSNLNTTFISYLNHIRIEKSIELLENTTKSINEIAEIVGYNSLNNFYKNFKIE
                     KGMTPKVYKLNTKN"
     gene            2800..3888
                     /locus_tag="ACOIAF_003729"
     CDS             2800..3888
                     /locus_tag="ACOIAF_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670707.1"
                     /translation="MSDKVNMKNIICIAGAYLATAIGSGFATGQEILQFFASQGYMGL
                     IGALISMVLFSFLGAEVITRGRELKLKEPTRIYTFYCGKILGKFYEWFSPIFLFGILI
                     VMISGAGSTLTEYYGINPYLGRVLMAVATLITVSMGLEKLVSILGRIGPVIAVFTIAV
                     GLISLFKHFDGLSMVGEAMKNIEVTKASESPIVSGVIYNTLNIIVMIVFLTGMGASIE
                     SKKDAFWGGLLGGIVFMTAGIVMYLAMISDIGNLYMKEIPSLYLADNISPIVGVCFSV
                     ALILGIYTTAVPLLWSVTNRIVEDEHPKFKLVTTVIAILACIGGFLPFGKLVNILYPY
                     TGYMGLLILVCMVYKAITCRKKSTQSQE"
BASE COUNT         1603 a          391 c          644 g         1383 t
ORIGIN      
        1 tagaagtaat aaaaaaatag ttaatataaa agtgatacaa taataataga agtaataaaa
       61 aaaaataatt aatataaaaa tgtgtacaaa ctaatgaata ttgatatagt aagctatcat
      121 atgaaagagt atgtacacat ttttttacta atttttcaca atatgaacat aaataattta
      181 caggaaagga atcttttatg aaaattcaaa atactgagtg gggatatatt gaatggaaac
      241 atacttatga tgaaaataat ccaaaacaag ctatgaatat atacattgca gtaactatgc
      301 ctggtaagaa acattttaat catgttcatt atggtcaaga gcagatgata tatattttag
      361 agggagaggg tttgtatatt ataaatggag tttggaagcc attttatcaa ggtatgattt
      421 tttatataga atctggtagc acacatgaaa ctataaatac aggtgataga gaaataaaag
      481 aacttattgt ttcaaataat gtagatgatg taggagagag tgaagttata gatattaatc
      541 ctaataatta tttaaagaag accttgataa attatagtga aagtacatta aatttgtatg
      601 cagcagtaga atcaattcgt ggtcaattta tagacccgtt taaaattcca ttaattatat
      661 atgatgattc atggaatata gttttaaaaa atccttattt tccacttttt tgttttgaaa
      721 aatgtaatcc tatgaaattt cctcaaaatt gtgattgtat gaatcaaaaa agtagcaatc
      781 aatttgtatg tgaatatggg ataactatat ataatatacc aatactgtac aaaaacaata
      841 gcataggtgt tataagagga gggtatgtac tattatctga tttgaattta gatacagagc
      901 ataataattt atatgatatt ccagagggag ctgctagaag tataaaacgc ctcttgaaac
      961 aaatatctaa aaatataata aatttttgct cttttaatga tataagaaag gatttacaag
     1021 aaaaagagaa gactatagca agaacatatc attatggaga acaacttgaa atgaatttaa
     1081 aagtagctca agatatggtt actaatttaa gaataaatca tcacttttta tttaatacat
     1141 taaattcaat ggcaagcata gcgctagatg atggtagtta tgatttgtat agtgcaataa
     1201 tagatttatc aagaatgttt agatatacaa tgaggtctga tttaagattt gtagaattag
     1261 aatctgaaat attatacatt aaaaattatt tgaatttaca gaaattacgt tatggagatg
     1321 cacttaaagt aaaatatctt attccagaga agttatataa cctttctgtg ccttttaact
     1381 ttattcaacc tattgtagag aatgcattta cacatggatt tagagatatt gacacagaaa
     1441 agcgaattga aataattgca agattagatt ctcagtatgc tattatagag attcataata
     1501 atggaactat cttagatgga aataatatag acaaaataaa ggcaggtatt cgtagtaata
     1561 atggtcatgg tttgtcttta atatatacaa aattcacatc tgcttatgga aataattttg
     1621 atatggatat aaagtcgagt gataatgaag gaacttacat aatgattaaa attcctattg
     1681 aaaattataa ggagtatgta tagaacttgt gaaaaattaa taagttttat aaatgttaag
     1741 aaatatatta gatgttttta tgttgtttta tatatcttac ttttttatat tattaagcta
     1801 aaagggaggt attctcatga gaagagcact aataatagat gatgaagaag ctgttctaaa
     1861 aataataaca cattttatag aaatgaaaga tatgccaata aaaattgttg gtaaagcaac
     1921 atctggtgat gaagcagtag ataaaatcat tggtttagag ccagacatag tttttataga
     1981 tattcaaatg ccaatataca acgggttaga agtaatagaa aaaacatctc atttatctaa
     2041 aaaaataaac tttgtagtaa taactgcatt taactatttt gaatatgctc aaaaagcttt
     2101 gagattaaat gtaaaagata tactccttaa accattggat atgaaagaat ttactaagtc
     2161 tgtagaaaaa attattggat atcaatatac caataatgat ttattaaatg agatactgga
     2221 atatataaat ctaaactata gcaatgactt aaagttaaat gattgtgcta gattattttt
     2281 aaccaatcca tctaatatat ctaggatttt taaaagtaat ttaaatacaa cttttatatc
     2341 atatctaaat catataagaa tagaaaaatc tattgagtta cttgaaaata ctacaaaaag
     2401 tataaatgag attgcagaaa tagttggata taatagccta aataactttt ataaaaactt
     2461 taagatagaa aaaggaatga ctccaaaagt ttataaactg aacactaaaa attgaaattg
     2521 ataaataaaa agtcaatata gataattgtt tgagtgtgtc tgaataagta tactttaatt
     2581 atgataaaaa ttaaagcgct ttaaacatat ttaataatat taaaccttat aaaagctgta
     2641 cctatttata taaaaaaata tatataaata aaaagaaaaa gatatacaaa ataaaaaaag
     2701 tgaattaaat actgtttcaa aaagacagaa ttttcattta atttactgag agtttatatt
     2761 tcttaaagga aggtaacttt ataatggagg taatttaata tgtcagacaa agtgaatatg
     2821 aaaaatatta tctgtattgc aggcgcttat ctagcaacag caattggttc tgggtttgct
     2881 actggtcaag aaattctaca attttttgcc tcacaagggt atatgggttt aattggagca
     2941 ttaatatcaa tggtattgtt ttcatttctt ggtgctgaag ttataactag aggtagagaa
     3001 ttaaaactta aagaaccaac aagaatttac actttttatt gtgggaagat tttaggaaaa
     3061 ttttacgaat ggtttagtcc aatattttta tttgggattc ttatagtaat gatttcaggt
     3121 gcaggctcaa ctttaactga atactatggt ataaatcctt acttaggaag agtattgatg
     3181 gctgttgcta ctttaattac agtgtctatg ggtttagaaa aattagtaag catacttgga
     3241 cgtattggac cagtgatagc agtatttaca attgctgttg gattaataag cttgtttaag
     3301 cattttgacg gtttatctat ggttggagaa gctatgaaaa atattgaagt tacaaaagca
     3361 tcagaaagtc caatcgtttc tggtgtaata tataatacat taaatattat tgtaatgatt
     3421 gtttttttaa caggaatggg agcttctatt gaaagtaaaa aggatgcttt ttggggaggt
     3481 ttattaggtg gaatagtgtt tatgactgct ggtatagtta tgtatttggc tatgatttca
     3541 gatataggaa atttgtatat gaaagaaata ccatcacttt atttagcaga taatatatct
     3601 ccgattgtag gtgtatgttt ttcagttgca ttaatattag gtatctatac aacagctgtt
     3661 ccacttttat ggtctgtaac caatagaatt gttgaagatg aacatcctaa atttaaattg
     3721 gtaacaacag taatagctat tttggcttgt ataggaggtt ttttaccgtt tggtaaatta
     3781 gtaaacattt tatatccata tacaggttat atggggcttt taattttagt ctgtatggtg
     3841 tataaagcaa ttacatgtag aaaaaaatca acacaaagtc aggaatagag ccacttatta
     3901 atagttttta tttaaaaaaa ttttttatat aatccgaatt taaaaagtca aaatagttaa
     3961 ataaattgca aaacatgata atatatttaa ttaaaataat atagtaaaat ataaatataa
     4021 a
//
LOCUS       DBGJFF010000220         3938 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.225, whole genome shotgun sequence.
ACCESSION   DBGJFF010000220 DBGJFF010000000
VERSION     DBGJFF010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3938
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.225"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            48..251
                     /locus_tag="ACOIAF_003730"
     CDS             48..251
                     /locus_tag="ACOIAF_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGZ2670708.1"
                     /translation="MISYAPLWKLLIDRKIKKMEFVNISGISISVLGRLGNDKSVSMD
                     TMEKICLALDCKIEDVVEIKKGI"
     gene            415..1569
                     /locus_tag="ACOIAF_003731"
     CDS             415..1569
                     /locus_tag="ACOIAF_003731"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HGZ2670709.1"
                     /translation="MKKISILGSTGSIGKQTLDVVRDNRDKFEIVAISANSNIELLLE
                     QIVEFKPKYVTVFEENKALKLKEMLPKNIEIEVLAGMEGLKIISSLDEVDVLLTAVVG
                     MIGLVPTLCAIKKGIDIALANKETLVTAGELVMKEAEKYNVNILPVDSEHSAIFQCLN
                     GENKKNIEKIILTASGGPFRGKKKGELVNITKNEALKHPNWSMGRKISIDSSTLMNKG
                     LEVIEARWLFGVEQENIDVVVHPQSIIHSMVQYTDSSIIAQLGCPDMRLPIQYALTYP
                     DRMESSFERMNFSKFSTLTFEEPDLETFPCLKLAYECLKMGGTYSSVLNSANEVLVSE
                     FLEDKIGFYDIPYYIEKTLEVHSSISEPTLEEILETDRWSRAYVANLIKK"
     gene            1582..2586
                     /gene="rseP"
                     /locus_tag="ACOIAF_003732"
     CDS             1582..2586
                     /gene="rseP"
                     /locus_tag="ACOIAF_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HGZ2670710.1"
                     /translation="MTIIAALILFSIIVLIHELGHFIFAKRSGIKVNEFSIGMGPKIY
                     SVKKDTEYSIRALPIGGYVSMEGEDEEQISPNSFGNKSILQRFSTIVAGPIFNIILAA
                     ILLVPVFLYIGSPTTKLGKIMPDTPAQAVGLQVGDKINKINGNSVKTWDEVANIINTS
                     SGGELKLSITRDGSDKVVNVTPKNNNGKYEIGIQPQREKDFLGSIVNACKTTVDMTKQ
                     MLTFLGQMITGRVPGGIGNAVAGPVGVIGMVSDAARTGIINVVYLAAVISLNLGIVNL
                     LPIPALDGWRILMLLLEAVRGGKKLDPNKEGMINVVGFGALMLFMLFITYKDILRLFQ
                     "
     gene            2769..3824
                     /gene="ispG"
                     /locus_tag="ACOIAF_003733"
                     /gene_synonym="gcpE"
     CDS             2769..3824
                     /gene="ispG"
                     /locus_tag="ACOIAF_003733"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HGZ2670711.1"
                     /translation="MSYERRKTREVSVGTVKIGGENPISIQSMTNTDTRDADATIAQI
                     KRLEEAGCDIVRVAVPDIKAAKNIAKIKSSVNIPIIADIHFDYKLALEAIKQGVDGIR
                     INPGNIGSIERVKMVVEKCKERNLKIRIGVNGGSLEKELLKKYGSATAEALVESAMGH
                     IKILEDLDFHNIVISLKSSDIYKTVDAYELISKKVDYPLHIGITESGSVHKGTIKSSI
                     GVGALLLKGIGDTVRISLTGDPVEEVIVGKQILRSLGLLNDKIKVISCPTCGRCNIDL
                     ISVVNEVEEKIGSMEKNITVAIMGCAVNGPGEAREADIGIAGGKGEGLLFKKGEIVRM
                     IDGNKLVDELLEEIEKL"
BASE COUNT         1527 a          394 c          774 g         1243 t
ORIGIN      
        1 tccaaaatag ataaaatgtg atagaatata ttttgcgagg tgataacatg atttcatacg
       61 ctccattatg gaaattgtta attgacagaa aaataaagaa aatggaattt gtaaatatat
      121 caggtataag tatttctgtt ttaggcagac taggaaatga taaatctgtt tctatggata
      181 caatggagaa gatatgttta gcactagact gtaaaataga agatgttgtg gaaataaaaa
      241 agggcattta ataatactag aatattttat aatattccac aaaatacaaa aaataataga
      301 ttaattttaa tctgtggaat aatatatgaa atttctatga atagtaaaaa taattgtttt
      361 agtagtgtta taatgttttg acagggtata tttattatta gaaaggatta ttatatgaaa
      421 aagatatcaa ttttaggttc tacaggctct ataggtaaac aaactttgga tgtggttaga
      481 gataatagag ataagtttga gatagttgca atatcagcta acagtaatat tgaattattg
      541 ctagaacaga tagtagagtt taaacctaaa tatgtaacag tttttgagga aaataaagca
      601 ttgaaattaa aagaaatgtt gcctaaaaat atagaaatag aagttttagc aggaatggaa
      661 ggattaaaaa taatatcatc acttgatgaa gtggatgtcc ttttaactgc tgtagtaggt
      721 atgataggat tagtcccaac tctttgtgct ataaagaagg gaatagacat agcattagcg
      781 aataaagaaa cgttggtaac tgctggtgaa cttgtaatga aagaagcaga aaaatacaat
      841 gtaaatattc ttcctgttga tagtgaacat agtgctatat ttcaatgtct aaatggagaa
      901 aataaaaaaa atatagaaaa gataatactt acagcatctg gtggtccatt tagaggaaag
      961 aaaaagggag aacttgtaaa tataactaaa aatgaagctt tgaagcatcc aaattggagt
     1021 atgggaagaa aaataagtat tgattcttca acgcttatga ataaaggact tgaagtgata
     1081 gaagctagat ggttatttgg agtagaacaa gaaaatatag atgtagtagt tcacccacaa
     1141 agcataattc attcaatggt gcagtataca gatagctcaa taattgctca attaggatgt
     1201 ccagatatga gactaccaat acagtatgct cttacatatc cagatagaat ggaaagtagc
     1261 tttgaaagaa tgaatttttc aaaatttagt actttaactt ttgaagaacc agatttagaa
     1321 acttttccat gtttaaaatt ggcatatgag tgtttaaaaa tgggaggaac ttattcttct
     1381 gttttaaatt cagcaaatga agttttagta agtgaatttt tagaggataa aattggtttc
     1441 tatgacatac catattacat agagaagact ttagaagttc atagtagcat aagtgaacca
     1501 acattagaag aaatcttaga aacagataga tggagtagag cttatgttgc aaatctgata
     1561 aaaaaatagg aaggtgaatt tatgactata atagccgcat taatactatt tagtataatt
     1621 gtattaatac atgagttagg acattttata tttgcaaaaa gaagtggtat caaagtaaat
     1681 gagttttcta taggtatggg gcctaagata tatagtgtaa aaaaagatac agagtattct
     1741 ataagagcac ttccaattgg tggatatgtc agcatggaag gtgaagatga agaacaaata
     1801 agtccaaatt cttttggaaa caagtctata ttacaaagat ttagtactat tgtagcagga
     1861 ccaatattca atataatatt agcagcaata cttttagttc cagtattttt gtatataggt
     1921 tctccaacga ctaaattagg aaagataatg ccagatactc ctgcacaggc tgtagggctt
     1981 caagtaggag ataagataaa taaaattaat ggtaattctg ttaagacttg ggatgaagtt
     2041 gcaaatataa taaatacttc ttctggtgga gaattaaaac ttagtataac tagagacgga
     2101 agtgataaag ttgtaaatgt aacacctaaa aataacaatg gaaagtatga aatagggatt
     2161 caaccacaaa gagaaaaaga ctttttaggg tcaatagtaa atgcatgtaa aactactgta
     2221 gatatgacaa aacagatgct aacatttcta ggtcaaatga ttacaggacg tgtaccagga
     2281 gggattggca atgctgtagc aggaccagta ggtgttattg gtatggtatc agatgctgct
     2341 cgtacaggca tcataaatgt agtgtattta gcagctgtca taagcttaaa tttaggtata
     2401 gtaaatctat taccaatacc agcacttgat ggatggagaa tacttatgct gttactagaa
     2461 gctgttagag gtggtaaaaa actcgaccca aataaagaag gcatgataaa tgttgttggt
     2521 tttggagctt taatgttatt tatgcttttt atcacatata aagatatact aagattattt
     2581 caatgattta gttttttaga gattttaaga aaataggcta tgtagtttta aataagaata
     2641 aattaatttt tatgtatgaa atttattctt atttaactag atagcttttt aatttataat
     2701 atacatgtag gttataatat tttaatattt aaagagattc aatataaaat aatatataga
     2761 ggtggaatat gagttacgag agaagaaaga cgagagaagt aagtgtagga actgtaaaaa
     2821 taggaggaga aaatcctata agtatacaat ctatgacaaa tactgataca agagatgcag
     2881 atgctacaat agctcaaata aagagattag aggaagctgg ttgtgatata gttagagttg
     2941 ctgttcctga tattaaagct gctaaaaata tagcaaagat aaagtctagt gtaaatatac
     3001 caattattgc agatatacat ttcgattata agctagccct tgaagctata aaacaaggtg
     3061 tggatggaat aagaataaat cctggtaata tcggaagtat agagagagtt aagatggtag
     3121 ttgagaaatg caaggaaaga aatttaaaaa ttagaattgg agttaatgga ggttctttag
     3181 agaaagaact tttaaaaaag tatggctctg caactgctga agcattagtt gaaagtgcaa
     3241 tgggacatat taagattctt gaagacttag attttcataa tatagttata tctctaaaat
     3301 catccgatat ttataaaact gttgatgcat atgaattaat atcaaaaaaa gttgattatc
     3361 ctcttcatat tggaattaca gaatctggaa gtgttcacaa aggaacaata aagtcttcta
     3421 taggggtagg agcacttctt cttaaaggaa ttggagatac tgttagaata tctttaacag
     3481 gcgaccctgt tgaagaggtt attgttggta aacagatttt aagaagtttg ggacttttaa
     3541 atgataagat taaagttata tcatgcccta catgtggtag atgtaatata gacttaatta
     3601 gtgttgtaaa tgaggttgaa gaaaagattg gtagtatgga aaaaaatatc actgttgcaa
     3661 ttatgggctg tgctgtaaat ggacctggtg aagccagaga agcagatata ggtattgctg
     3721 ggggtaaagg tgaaggtctt ttatttaaaa agggtgaaat agttagaatg attgatggta
     3781 acaaattagt tgatgagttg ctagaagaga ttgaaaaatt atagtataca attcatatat
     3841 atattaaatg actagatttt taaatacttt tgtaataagt ttttatttac agtatttaat
     3901 tatctagtca ttttttattt tataaacttc attaaatg
//
LOCUS       DBGJFF010000221         3936 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.226, whole genome shotgun sequence.
ACCESSION   DBGJFF010000221 DBGJFF010000000
VERSION     DBGJFF010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3936
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.226"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(275..874)
                     /gene="recR"
                     /locus_tag="ACOIAF_003734"
     CDS             complement(275..874)
                     /gene="recR"
                     /locus_tag="ACOIAF_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HGZ2670712.1"
                     /translation="MQVYTGPITRLIEEFSKLPGVGRKTAQRLAFHIINMNTNDVEAL
                     SKAIIDAKREIRYCSICCNITDTDPCSMCSNKSRDSSVICVVEDPRDVAAMERTREFK
                     GQYHVLNGVISPMDGIGPDMLKIKELIQRLGNQEVKEIIMATNPTIEGEATAMYIARL
                     VKPMGIKVTRIAHGLPVGGDLEYADEVTISKALEGRREI"
     gene            complement(887..1237)
                     /locus_tag="ACOIAF_003735"
     CDS             complement(887..1237)
                     /locus_tag="ACOIAF_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HGZ2670713.1"
                     /translation="MAKKGFGGGMMPGGGNMNNLLKQAQKMQENMQKAQQELESKEVE
                     ASVGGGAVTVKVNGKKEVIDITIKPEVVDPDDIEMLQDLVLSAVNQALRNIDDIQASQ
                     MSKVTGGMNIPGLF"
     gene            complement(1296..2933)
                     /gene="dnaX"
                     /locus_tag="ACOIAF_003736"
     CDS             complement(1296..2933)
                     /gene="dnaX"
                     /locus_tag="ACOIAF_003736"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HGZ2670714.1"
                     /translation="MHKALYRAYRPQKFEDVIGQDHIIKTLKNQIYSDNIGHAYLFCG
                     TRGTGKTSTAKIFSRAVNCLNKINEEPCNECEICESVLKDNTMDVVEIDAASNNSVDD
                     IRELRESVKYSPANAKYKVYIIDEVHMLSQGAFNALLKTLEEPPSYVIFILATTEPHK
                     IPATILSRCQRYDFKRVTVKDMTLRMKKICEDEGIDIDDKALNLIARNSQGALRDALS
                     ILDQCMSFGESKIDYKDVVELMGSVNIEQLFELSQCIVEQDTKKSLEILNEFVLWGKD
                     IRNLINDLIDHFRNLMVCKVSSELDEIISLPEETIEQLKIQSKNIDINDLIRILNILS
                     ITQDDIKSSSNPRVLVEITIMKIAQPMFDESKEALIKRVENLEKMIELGNFKSEKIGN
                     NKEKEYEVDREIDVKQENVVYEDVKNEDVILIESSWKNILKQIKKDKKMPIYALLSEV
                     KSFNVYSNMLYVIFDDKFDFAKTRLSSQDTINYLEKTIRDVLNRSFNVKIVLTSEVKD
                     INLEVKEKKDIGEEILKNIVSEEILEIKDSIDENESK"
     gene            complement(3010..3274)
                     /gene="ffs"
                     /locus_tag="ACOIAF_003737"
     ncRNA           complement(3010..3274)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACOIAF_003737"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(3312..3403)
                     /locus_tag="ACOIAF_003738"
     tRNA            complement(3312..3403)
                     /locus_tag="ACOIAF_003738"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3367..3369),aa:Ser,seq:gga)
     gene            complement(3404..3859)
                     /locus_tag="ACOIAF_003739"
     CDS             complement(3404..3859)
                     /locus_tag="ACOIAF_003739"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HGZ2670715.1"
                     /translation="MESSFYMKEALKEAYKAYNKKETPIGAIIVKDNQIIARAHNLTE
                     TLKDSTVHAEILAIKQASEKLGGWRLTDCDLYVTMEPCIMCSGAIVNSRIKKLIIGTR
                     HVKNSYIEKQHEFKLDYFNNNNVKVAFDVLQEECSIILQEFFKALRKRD"
BASE COUNT         1156 a          784 c          416 g         1580 t
ORIGIN      
        1 tttttagttt ttttcttatt agaaaaactt atatcctcaa aatcatcata tttaggttta
       61 tcttttgatt tcattttttt aactcttttt tctttcattg cattatgttc ttctaagttc
      121 ataattggtt tcatgttgca cccctatcaa aatttttaaa tgatttatac aacaaattat
      181 acaatacaga agaaatttat tcaataaatc taaatcaaaa ttaatttgaa tttaactcaa
      241 aaaattacaa aaatcgaggt attaacctcg atttttatat ttctcttctg ccttctagtg
      301 cttttgatat tgtgacctca tctgcatact ctaaatcgcc tccaacagga agaccatgag
      361 ctatccgagt gactttgatt cccattggtt ttactaacct agcaatatac atggctgtag
      421 cttcaccttc aatagtagga tttgttgcca ttattatttc tttgacttcc tgattgccaa
      481 gtctttggat aagttccttt atttttaaca tatctggtcc aattccatcc attggagaaa
      541 ttactccatt taatacatga tactgtcctt taaactctct cgttctctcc atagctgcta
      601 catctcttgg atcttccaca acacatataa cacttgaatc tctactttta tttgaacaca
      661 tgctacatgg gtctgtatct gttatattac aacaaattga gcaatatctt atttctcttt
      721 ttgcatctat aatcgcttta gataaagctt ccacatcatt agtattcata ttaataatat
      781 gaaaagccaa tctttgagca gtctttcttc ctactccagg aagctttgaa aattcctcta
      841 taagtctcgt tatgggacct gtataaactt gcataaatac cacctactag aataacccag
      901 gtatattcat acctccagtt accttactca tttgacttgc ttgtatatca tcaatatttc
      961 tcaatgcttg atttactgca cttaaaacta aatcttgtag catttctata tcatctggat
     1021 caactacttc tggcttgata gttatatcta taacttcttt tttaccatta acttttacag
     1081 taactgctcc tccaccaact gaagcttcta cttctttact ttctaactct tgttgagctt
     1141 tttgcatatt ttcttgcatt ttttgagcct gctttaacaa gttgttcata tttccaccac
     1201 caggcatcat tccgcctcca aaaccttttt tagccataaa cactattcct cctaaactta
     1261 ttttcatttt attattatat cataatagta ttttattatt tactctcatt ttcatcaata
     1321 ctatctttta tttctaatat ctcttcactc actatgttct ttaatatttc ctcaccaatg
     1381 tcctttttct ctttaacttc aagatttata tctttaactt ctgatgtcag tactattttt
     1441 acattaaaac ttctatttaa aacatctctt attgtttttt ctaaataatt tattgtatct
     1501 tgagagctca gtctagtttt tgcaaaatca aatttatcat caaatataac atacaacata
     1561 ttagaatata cattaaaact ttttacttca cttaagagtg cgtatattgg cattttttta
     1621 tcttttttaa tttgtttaag tatattcttc caagatgact ctataagtat aacgtcctca
     1681 ttttttacat cttcataaac tacattttct tgttttacat ctatttctct atcgacctca
     1741 tactcttttt ctttattatt cccaattttt tcagacttaa agttccccag ttcaatcatt
     1801 ttttctaagt tctcaactct ttttataaga gcttctttac tttcatcaaa cattggttga
     1861 gcaattttca ttatggttat ttccactaaa acccttggat ttgaagatga ctttatatca
     1921 tcctgagtta ttgataatat atttaaaatc cttattaaat catttatatc tatattttta
     1981 gactgaattt taagttgttc tatagtttct tctggtaatg aaattatttc atccaattct
     2041 gatgaaacct tacataccat taaatttcta aaatgatcaa ttaagtcatt tataagattt
     2101 cttatatctt tgccccataa gacaaattca tttaatatct caagagactt tttagtgtcc
     2161 tgctctacaa tacattgaga caattcaaat aattgttcta tatttacgct tcccattaat
     2221 tcaacaacat ctttataatc tatcttactt tctccaaaag acatacattg gtctaatata
     2281 ctaagtgcat ctctaagagc tccttgagaa tttctagcta ttaaattaag agctttatca
     2341 tctatatcaa ttccttcatc ttcacaaatc tttttcattc taagggtcat atctttaact
     2401 gtaactcttt taaaatcgta tctttggcat cttgataaaa tggttgcagg tattttgtga
     2461 ggctctgtag ttgctaatat aaaaattaca tatgatggag gttcctctaa ggtctttaat
     2521 aatgcattaa aagctccttg agacaacata tgaacttcat ctattatata tactttatat
     2581 ttagcattgg ctggagaata tttcacactt tctctaagct ctcttatatc atctacacta
     2641 ttatttgaag ctgcatctat ttctacaaca tccattgtat tatcttttaa cacagattcg
     2701 catatctcac actcattaca cggttcttca tttattttat tcaaacagtt tactgctctt
     2761 gaaaaaatct ttgcagtaga tgttttacca gtacctcttg ttccacaaaa caaataagca
     2821 tgtcctatat tatcactata tatttgattc tttaaagtct taataatatg atcttgacca
     2881 ataacatctt caaatttttg tggtctatat gctctatata atgctttatg catcttttta
     2941 cctctcttta gcaaaattaa taaaaaaatc cctcattatc taagggatta gtcttttata
     3001 tattaaatgc tgtacaccat ctatcgactt aattccatag gcgttaccaa aacagttagc
     3061 tcaacttagg cacccccacg gcacacaaaa gtgatcactt accgctgctt ccttccggat
     3121 ctgacggggt tcatgagctt tcattgcgca ggacctaagc atcaacacca cttatttagg
     3181 gcggccctac agaaaataag cctttagatg gaattcaatc ctgctatagc ggattgcagg
     3241 ttacagggca ccgctacctc cccatctagt acagcaaaat tacaaccata tttaattact
     3301 ttcattgttt ctggcggaga gagtgggatt tgaacccacg atacagtttt acccatatac
     3361 tcgctttcca ggcgagcgcc ttcgaccact cgaccatctc tccttaatct ctttttctca
     3421 atgccttaaa aaattcttgc aatatgatcg agcattcttc ttgcaaaaca tcaaaagcta
     3481 cttttacatt attattatta aaataatcta atttaaactc atgctgtttt tctatatatg
     3541 aattttttac atgtcttgtc cctattataa gcttttttat tctagaatta actattgcgc
     3601 cactacacat tatgcaaggt tccattgtaa catataaatc acaatcagtc aacctccaac
     3661 cacctaattt ttctgatgct tgttttatcg ctaaaatttc tgcatgtacc gtagagtctt
     3721 ttaacgtttc tgtaaggtta tgagctctag ctattatttg attatcttta actataatag
     3781 cacctattgg tgtttctttt ttattatatg ctttatatgc ttcttttaat gcttctttca
     3841 tataaaatga actttccata attcctccca aaacttataa caaacttatc ttatcataat
     3901 tcattttata attcaactta attttatttt gtttat
//
LOCUS       DBGJFF010000222         3869 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.227, whole genome shotgun sequence.
ACCESSION   DBGJFF010000222 DBGJFF010000000
VERSION     DBGJFF010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3869
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.227"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            967..1719
                     /locus_tag="ACOIAF_003740"
     CDS             967..1719
                     /locus_tag="ACOIAF_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2670716.1"
                     /translation="MLKVNQLCKSYKTGSKTYPVLKNISFEINKGEFVAIMGSSGSGK
                     TTLLNCISCFIPIDNGEIILGNNNIRNLKEKELSEVRNQQLGFVFQDFMLFDGLSVFE
                     NICVPQIIKGSQVLQMENRAINLCELFGIEHIRKKYPAEISGGEKQRTAVARALMNNP
                     DLILADEPTGNLDSKSCRTVIESFLKAKQELDATILMVTHDSFAASFCDRVIVLKDGI
                     IYKEVVKSNSRHKFLDELLEVLKEMGGAQDDN"
     gene            1709..3820
                     /locus_tag="ACOIAF_003741"
     CDS             1709..3820
                     /locus_tag="ACOIAF_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2670717.1"
                     /translation="MTINKLYSKLRAYNWKNYTTLFFCIILSVVLVTSYALIYFSPTI
                     QNILPTGGDSRKQATMIFSVAILGCTVFTTYASSLFLKFKSKEIGIFMALGTDKKKLK
                     KLLFSEILLISVVCCFLGLILSIPVSFGIWKIFQLVIIDTREMTYHFGTQGFIYGFIF
                     SVFVTLCIFILSVKFINRTNIIDILNDQRISEPIQDVKDWYGILGIIFIILGIFLGYL
                     VPSLTINKLNYRLPSVWSFTYLLSLIGIYMIMTYIVVHAKRGKHPEKYYKNIISTSMM
                     RFMGRQTVKNMCVISFLIAGALFAVFYVPNMVTGIYDTLKNNPIDYTFTYNQRDKQVS
                     KKDIEQLADKHNIKIQNYMEIPSIELIVDGVESIYHKDGTIEKVYMKKSSYDMFFNSK
                     DFSKLIGKPITIKNGEYLAVVNKGNENQDYKYTKFISSPVTEKEIPFRYAGKIIFNGS
                     FFQNRSMNAYVLNDEDYKSFTKDLSIKNYNNSILFNVDKDTYAFANDLKKEIIKRTSK
                     EMGMFSGYDEYVKKRYESTGRKYFMDEEYPSGEGHLKLDPNNNQLYLNWKYYPNFKIL
                     QSQDMIKNMAVFLMLFIYVAIICFTAVGIIAYTRGVTIAINHKQVFMDLNRLGANNDY
                     IKFCIRNQLKKVFSYPLIAGSFLIYLFNFMIMLNNDGRIVNSEVNALLINLGFIGICG
                     VYMYLIYLLTYKKFKKIIGIL"
BASE COUNT         1438 a          560 c          483 g         1388 t
ORIGIN      
        1 catttctgtt acattgcaaa gtcttattta ttacagttct ttattatact acagactttt
       61 attattacaa tctcttattt attacagttc tttgctatac tacagacctt tattattata
      121 acatttctat tatattacaa tctcttatta ttacagttct ttattatact acagactttt
      181 attattacaa tatttttatt atattacaat ctcttattta ttacagttct ttgctatact
      241 acagaccttt attacaacat ttctattata ttacaatctc ttatttatta cagttcttta
      301 ttatactaca gacctttatt attacaacac tttattattt taacttcttt actataatat
      361 attaatattt taatatctca aaatcatatc aatcactttt cagcatagtc taaaatactt
      421 ccactttcta aaattatcta tcatacctat actaataata ctttttaacc tttttattat
      481 acatttcaat tgtttttaac aacctattta tttccaaaac tccaatatat tttctcaatt
      541 acattgtgtt aataacccct aaatcctttc aaattattta caatacattc cattaaataa
      601 tcttactgct ttataattct ttttttcttt ttaaccctac tatacattat ttacatcatt
      661 tttttcaata caacattcgt tattctataa cctttttact tttactatat acttcttttc
      721 taactacacc ttcaatgttc cacatcatat tactttagca caacaattaa tacctaaaaa
      781 taaccctcta aatcctccaa taatataatc aatttaacta taataataac ctctcacact
      841 atccaaacaa tatattttca aacattacaa aaaaacatcc aactattaca atatagctca
      901 tatccctcct cccctttcaa atgctatcat tgacttacaa gataaatgaa agtgaggatg
      961 atatatatgc ttaaagtaaa ccaattatgt aaatcatata aaacaggcag taaaacatat
     1021 cctgtattaa aaaatatttc ttttgaaatc aacaaaggag aatttgtcgc aatcatgggt
     1081 tcatcaggaa gtggaaaaac tactctttta aattgtattt catgttttat cccaattgat
     1141 aatggggaaa taattcttgg aaataataat ataagaaatt taaaagaaaa ggaactttca
     1201 gaagttagaa atcaacaact aggatttgta ttccaagact tcatgctatt tgatggtctt
     1261 agtgtctttg aaaatatatg tgttccacaa attatcaaag gtagtcaagt cttacaaatg
     1321 gaaaaccgtg caattaatct atgtgagcta tttggtattg aacatatcag aaaaaaatac
     1381 cctgccgaaa tatctggtgg agaaaaacag cgtacagcag tagcaagagc actcatgaac
     1441 aacccagatt taatattagc agatgaacca actggtaacc ttgatagtaa gtcttgtcgt
     1501 acagtcattg aatcattttt aaaagcaaag caagaattag atgctactat actcatggtt
     1561 acacacgata gctttgcagc aagcttctgt gaccgagtta ttgtgcttaa agatggaata
     1621 atttataaag aagttgtaaa atcaaattca agacataaat tcctagatga attactagaa
     1681 gttttaaaag aaatgggagg cgctcaagat gacaattaat aaattataca gtaaacttcg
     1741 agcctataat tggaagaact atacaacttt attcttttgt ataatacttt ctgtggtatt
     1801 agtaacttca tatgctctaa tttattttag cccaacaata caaaacatac ttccaactgg
     1861 tggagatagc agaaaacagg ctacaatgat attctcagtt gctatattag gatgcactgt
     1921 ctttactaca tacgcatctt ccctcttttt aaaatttaaa agcaaggaaa ttggtatttt
     1981 catggctcta ggaacagata agaagaaatt aaaaaaactt ctattttcag agatattgtt
     2041 aatttcagta gtatgctgtt ttttaggtct tatattaagt attcctgtat cttttggtat
     2101 atggaaaata tttcaattag tgattattga taccagagaa atgacatatc attttggaac
     2161 tcaaggattc atatatggat ttatatttag tgtatttgtg acattatgca tatttatact
     2221 ttctgtaaaa tttattaatc gtacaaatat aattgatata ttaaatgacc agagaatatc
     2281 tgaaccaata caggatgtaa aggactggta tggtatatta ggtataattt ttattatact
     2341 aggtattttt ctaggatatc tcgtaccttc actaactata aacaaattaa attatagact
     2401 accatctgta tggagcttta cttatctact ctctcttatt ggaatatata tgattatgac
     2461 atacatagtt gtacatgcta aaagaggaaa acatccagag aaatactata aaaacatcat
     2521 atcaactagc atgatgagat ttatgggaag acaaacagta aaaaacatgt gtgttatttc
     2581 atttttgatt gcaggagcat tatttgctgt attctatgtt cctaatatgg ttacaggtat
     2641 ctatgatact cttaaaaata accctataga ttatactttt acctacaatc aaagagataa
     2701 acaggtatca aaaaaagata ttgaacaatt agcagataaa cacaatataa aaattcaaaa
     2761 ctatatggaa attccttcta tagaattaat tgttgatggt gtagaatcaa tatatcataa
     2821 agatggaact atcgaaaagg tatatatgaa aaaaagctcc tatgatatgt tctttaattc
     2881 taaagatttt tccaagctaa ttggcaaacc aataactata aaaaatggtg aatatcttgc
     2941 tgttgtaaat aaaggaaatg aaaatcaaga ttataaatat accaaattta tatcaagccc
     3001 agtcacagaa aaagaaatcc cctttagata tgctggaaaa ataatattta atggttcttt
     3061 ttttcaaaat agaagtatga acgcctatgt ccttaatgat gaggattata aatcatttac
     3121 taaagattta tccataaaaa attacaataa ttctatacta tttaatgtag ataaagatac
     3181 ctatgctttt gccaatgact taaagaaaga aattataaaa agaacatcta aggaaatggg
     3241 catgttcagt ggttatgatg aatacgtaaa aaaacgttat gaatcaactg gaagaaaata
     3301 ttttatggat gaagaatatc caagtggtga aggtcattta aaactagacc caaacaacaa
     3361 tcagttgtat cttaattgga aatactatcc taactttaaa attttacaaa gccaagatat
     3421 gataaaaaac atggcagtct tcttaatgtt attcatatat gtggcaatca tatgttttac
     3481 agcagtagga atcattgcat atacaagggg tgttactata gctataaatc acaagcaggt
     3541 ctttatggat ttaaatcgat taggagcaaa caatgattat ataaagttct gtattagaaa
     3601 tcaattaaag aaagtatttt cataccctct tattgcagga agttttttaa tttatctgtt
     3661 taattttatg attatgttaa ataatgatgg tagaatcgtc aatagtgagg tcaatgctct
     3721 tttgataaac ttaggtttta tagggatttg tggggtttat atgtacctta tatatctatt
     3781 aacttataag aaattcaaaa aaattattgg tattttataa taaaaaaggt tattagattt
     3841 ttaaagtaat aaaatcattg gtgttttat
//
LOCUS       DBGJFF010000223         3778 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.228, whole genome shotgun sequence.
ACCESSION   DBGJFF010000223 DBGJFF010000000
VERSION     DBGJFF010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3778
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.228"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            92..1144
                     /gene="ytvI"
                     /locus_tag="ACOIAF_003742"
     CDS             92..1144
                     /gene="ytvI"
                     /locus_tag="ACOIAF_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HGZ2670718.1"
                     /translation="MSILENNFLYKLKNNTIFFCIYTLLFIFIYKAFPYIAPFFLGAI
                     IALMINPISQKLENKFHINKGISTLVLSFLAVAIVSTVTTIIVINSMKELMGFLNNIS
                     ANPEDISNTIMYFLNKINDFMKSFQEIANFDVEQLVNKFSGEVMQITKNLLTSILGLA
                     TSIPYIIIFIITLFIATYFIAKDLDKIENSFYNMFTVDVRKKVKNVKKEAGLSLVGYI
                     RAYTILMAITFFAIWGSFALFGLKYGLIVGFVGALMDLIPFLGIITIYLPVIVYYFLI
                     KNYFIAISMTVIFFVLSLIREILEPKLVSVNVGLNPLATLAAIFIGIQVKGIIGVIFC
                     LGLVCMHDILKKVDIF"
     gene            1235..2461
                     /gene="pepT"
                     /locus_tag="ACOIAF_003743"
     CDS             1235..2461
                     /gene="pepT"
                     /locus_tag="ACOIAF_003743"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HGZ2670719.1"
                     /translation="MKQKVVERFLKYVSFDTTSNSQCENCPSSEGQRVLAKYIVEELK
                     TMGVDDVSLDENSYIMATLKGNTDGVDTIGFISHLDTIEDVSGKDIKPRIIENYDGKD
                     IVLNEALNVITYVKDSPELEEFKGDDLIVTDGTTLLGSDDKAGIAEIVTAIEYLINHP
                     EIKHGDIKIGFTPDEEIGRGADLFDVEKFGAKYAYTLDGGIVGELECENFNAANATIT
                     IHGRNVHPGSAKNKMVNAIHIAAEISEMFPADERPETTEGYEGFWHLNSIGGNVENVS
                     MAYIIRDHCREKFENRKSIMIENIEKINKKYDNRVELDLKDSYYNMKEKIEPVMFIVD
                     IAKEAMEELGIKPRLVPVRGGTDGARLSFNGLPCPNIFTGGLNFHGKNECIPVSSMEK
                     ATKLIVRIAEKYAERV"
     gene            2668..3720
                     /locus_tag="ACOIAF_003744"
     CDS             2668..3720
                     /locus_tag="ACOIAF_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp18"
                     /protein_id="HGZ2670720.1"
                     /translation="MKIKSKKIIAFALSLGIMLPSCVYADTISKNQNAQVDMNNYLQG
                     RKSPKEEKLIGEDRFDTAIKISHSGWNNGSERVFLVNSNSLPDALASTPLASKLDAPI
                     LLTNKNSIPYNVTEEIKRLNPAEVILIGSEGAISSSVKESLEDMEVLVSRIGGADREE
                     TSLLLTRQLDDTGDLGVSKVAVVNGYNGLADATSISSPAASDNTAIIYTGKDSIRSEA
                     KNFITQNSTKTYIVGGEYSISKKLEGQLVNSERLAGTDRKDTNAKVLEKFYGKSSKVN
                     NMYFAKDGSGREADLVDGLAAGVLASKTKSPVVLASGSLSSAQKSFIKKVKADKFVQV
                     GGGKNSKPYAEALALQ"
BASE COUNT         1455 a          396 c          700 g         1227 t
ORIGIN      
        1 tttatacact tctttttatt ttgtattcaa ataaaactag tatgaaatgg acaaaataaa
       61 aatataaatt agaagaaagg agatttttaa gatgagcata ctagaaaata attttttata
      121 caaattaaaa aacaacacta tctttttttg tatatataca ttgttattta tttttattta
      181 caaagcgttt ccatacatag ctccattttt tctaggtgct ataatagcac ttatgattaa
      241 tcctatatca caaaagttag aaaataagtt tcatataaat aaaggtatct ctactcttgt
      301 tctaagtttt cttgcagtgg ctatagtcag tacagttact acaataatag taataaattc
      361 aatgaaagaa ttaatgggat ttttaaataa tatatctgct aatcctgagg atataagtaa
      421 tactattatg tattttttaa ataaaataaa tgactttatg aaatcatttc aagaaatagc
      481 caattttgat gtagaacaat tagtaaacaa attcagtggt gaagtaatgc aaattacaaa
      541 aaatcttctt actagtatac ttggattggc tacttctatt ccatatatta taatatttat
      601 aattaccttg tttattgcta cgtactttat agcaaaagac cttgataaga ttgaaaatag
      661 tttttataat atgtttactg ttgatgttag aaagaaagta aaaaacgtaa agaaagaggc
      721 agggctttca cttgttggat atataagggc ctatacaata cttatggcta taacattttt
      781 tgcaatctgg ggaagttttg ctttatttgg attgaaatac ggtctaatag tagggtttgt
      841 aggagcttta atggatttaa ttccattcct aggtataata acaatatatc ttccagtaat
      901 tgtttattat tttcttataa aaaattattt tatagctata agtatgactg tgatattttt
      961 tgttttgtct ttgattagag aaatacttga acctaaacta gtatcagtaa atgttggact
     1021 aaatccatta gctaccctag cagcgatttt tattggaatc caagtaaaag gaataattgg
     1081 agtaattttt tgtttgggtt tggtatgtat gcatgatata cttaagaagg tggatatttt
     1141 ttaattataa taattgatag atttttttaa aaataaaatt ttattcacag gtaaataaaa
     1201 aaatattaat cttattaaga aagagggtat atacgtgaaa caaaaagtag tggaaagatt
     1261 tttaaaatat gtatcatttg atacaacatc taattctcag tgtgagaact gtccatcttc
     1321 agaaggtcaa agagttttag ctaagtatat agttgaagag ctaaaaacaa tgggtgttga
     1381 tgatgtgagt ttagatgaaa atagttatat aatggctact ttaaaaggaa atactgatgg
     1441 tgtggatact ataggattca tatcacattt agatacaatt gaagatgtta gtggaaaaga
     1501 tattaaacca agaataatag aaaattatga tggaaaagac atagttttaa atgaagcgtt
     1561 aaatgttatt acgtatgtaa aggattctcc agagttggaa gaatttaaag gtgacgattt
     1621 aatagtcact gatggaacaa ctctacttgg ctctgatgat aaagctggta tagctgagat
     1681 agtgactgca atagagtatc taataaatca tccagaaatt aagcatggag atataaaaat
     1741 aggatttaca ccagatgaag aaattggtag aggtgctgat ttatttgatg ttgaaaaatt
     1801 tggagcaaaa tatgcatata ctttagatgg aggaatagtt ggagaacttg aatgtgaaaa
     1861 ttttaatgct gcaaatgcaa ctattactat acatggtaga aatgttcatc caggttcagc
     1921 taaaaataaa atggtaaatg ctatacatat agctgctgaa atatcagaaa tgttcccagc
     1981 agatgaaaga ccagaaacaa cagaaggata tgaaggattt tggcatttaa attctatagg
     2041 tggaaatgtc gagaatgtaa gtatggctta tattattaga gaccattgta gagaaaaatt
     2101 tgaaaataga aaatctatta tgatagagaa tatagaaaaa ataaataaga aatacgataa
     2161 tagagtggaa ttggatttaa aagattctta ttacaatatg aaagaaaaga ttgagccagt
     2221 tatgtttata gttgatatag ctaaagaagc tatggaggaa ttaggtataa aaccacgttt
     2281 agttcctgta agaggtggta ctgatggagc aagattatct tttaatggat taccttgtcc
     2341 aaatatattt actggtggat taaacttcca tgggaaaaat gagtgtatac cagttagttc
     2401 tatggagaaa gctactaaac taattgtaag aatagctgaa aaatatgctg aaagagttta
     2461 gttaaaaggt atttaaaaat taagcattta aagttataat agctatatca ttctttataa
     2521 gtcaagctga ttataagctt gtgttgtatt taaaatggta tagctatttt tgtaattatt
     2581 tttataaaag ataattattt ttagtttagg gtatcaataa aaagtatgaa tatcaatagg
     2641 tattaacatt caaaaggggg aattgtattg aaaattaaat ctaaaaaaat aatagcattt
     2701 gcactttcac ttgggattat gttgccaagt tgtgtatatg cagacactat tagtaaaaat
     2761 caaaatgcac aggtagatat gaacaattat cttcaaggta gaaaatctcc aaaggaggaa
     2821 aaacttattg gtgaagatag atttgataca gcgataaaaa tatctcatag tggttggaat
     2881 aatggttcag aaagggtgtt tctagttaac tcaaattcat tgccagatgc attggcttca
     2941 actcctttgg caagtaaatt ggatgcgcca atactgctta caaataaaaa tagtatacca
     3001 tataatgtaa cagaggaaat aaaaaggctt aatccagcag aagttatttt aattggaagt
     3061 gaaggagcta tttcatcaag tgtaaaagaa tctcttgaag atatggaagt attagtcagt
     3121 agaatcggtg gagcagatag agaagaaaca tctttacttc ttactagaca gctagatgat
     3181 actggtgatt taggtgtctc aaaagtggca gtggttaatg ggtataatgg actggcagat
     3241 gcaacttcaa tatcaagccc agctgcaagt gataatacag ctataatata tacaggtaag
     3301 gatagcataa gaagtgaggc aaagaatttt attacacaaa actcaactaa gacatacata
     3361 gtaggtgggg agtacagtat aagtaaaaaa cttgaaggac agttagtaaa ctcagaaaga
     3421 ctagcaggga cagatagaaa agatactaat gcgaaagttt tagaaaagtt ttatggaaaa
     3481 tcaagtaagg ttaataatat gtattttgca aaagatggaa gtggtagaga agctgattta
     3541 gttgatgggc ttgctgctgg agtattagca tcaaaaacaa agtcacctgt agtacttgca
     3601 agtggaagtt taagtagtgc acagaaaagt tttataaaaa aagtaaaggc tgataaattt
     3661 gtacaagttg gtggaggtaa aaatagtaaa ccatatgcag aagcattagc cttacagtaa
     3721 tattgtgtaa atcaaaatta atatataata atagcaagta aaccaagttg cacttcaa
//
LOCUS       DBGJFF010000224         3756 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.229, whole genome shotgun sequence.
ACCESSION   DBGJFF010000224 DBGJFF010000000
VERSION     DBGJFF010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3756
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.229"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(151..1401)
                     /locus_tag="ACOIAF_003745"
     CDS             complement(151..1401)
                     /locus_tag="ACOIAF_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HGZ2670721.1"
                     /translation="MATIKPFKAIRPNKYIVDKVAALPYDVMNSKEARRIAEGNPYSF
                     LHIDKSEIDLDENIDLYDEKVYLKARENLDEFRKQEILVKDDKECIYIYKQIMDGRAQ
                     VGIVACISVDESLNGTIKKHEYTRPEKEIDRTKHIKYCDANTGTILVTYKHQRVIDDI
                     INDFMDNNEPLYDFITDDKIEHTVWKIDNDDIIDNLVDKFEKLDYLYIADGHHRTASA
                     ENVAKEMRAKNPNYTGKEEFNYFIAMIAPDENLMVLDYNRVIKDLNGLSEEEFINKIK
                     ENFELEEIEGKEKYKPDKKGTFGMYLGDKWYKMKANKNLLEIEDPVDSLDISILQDYV
                     IDAILGIDNPRVDKRIDFIGGIRGLEELEKRVNEDMKVAFAMYPTSIEDLIRVADANK
                     IMPAKSTWFEPKVRCGLFLHEINE"
     gene            complement(1433..2374)
                     /locus_tag="ACOIAF_003746"
     CDS             complement(1433..2374)
                     /locus_tag="ACOIAF_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HGZ2670722.1"
                     /translation="MYNILVTDGIEKEAARKLRELDFNVIEQFYEKDVLGDKLKDVDV
                     LVVRSATKVTKDVIDKALEGKKLKLIVRGGVGLDNIDVKYAQANGIKVMNTPNASSIS
                     VAELTIGQLFVLARFINTANVTMRDGKWEKKKYKGTEINGKTLGLIGFGRIAKEVAKR
                     AELLGMNVIYTDIMGEAQGFNNYKFCDMEEVLENADFLSLHIPFDKNKGAVITEKEIN
                     KMKKGAYLINCARGGLVDEKDLLKALDEGKLSAAAIDVYEQEPTLNLDLVNHPRVSPT
                     PHIGASTVEAQERIGDEIVNVIQDFFLDFNNLIGVAL"
     gene            complement(2614..3690)
                     /locus_tag="ACOIAF_003747"
     CDS             complement(2614..3690)
                     /locus_tag="ACOIAF_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HGZ2670723.1"
                     /translation="MSKKLFIPGPIDVKEEVLQKMATPMIGHRGKDASMLQKSISEKM
                     QKLFYTNNTILLSTSSGTGLMEGSIRSCTSKKAAVFSCGSFGDRWYKMAVANNVPADI
                     FKVELGEATTPEMVDKVLSTGEYDLITVTHNETSTGIRNPIEEIGEVVKKYEDVIYCV
                     DTVSSAGGIKVEVDKIGIDICITSVQKALGLPPGMSICTFSQKAIDRAKQVPFRGVYL
                     DLLAMYEYLIKKNYQYPSTPSLSHMFALDFQLDNILDEGLDNRFNRHEDMANLVRNWA
                     KKHFQIFTNENHLSNTLTVIENTQGISVSNLNSKLQERGFQIANGYGDLKEKTFRISH
                     MGDYTVEDVQELLDNIDDILGFNK"
BASE COUNT         1107 a          699 c          411 g         1539 t
ORIGIN      
        1 gactatctat atcctaaaat tatccatttc taaaaaaaag gtaactatta ttaagaatat
       61 caacttatag aatttatatt ccacatacga ttttcaatca aaaaaacaag ggctaaaaag
      121 tgaattgtac actttttagc cctttattaa ttattcattt atttcatgta agaatagccc
      181 acatcttact ttaggttcaa accatgttga cttagctggc attatcttgt ttgcatctgc
      241 tactcttatt aaatcttcaa tactagtagg atacattgca aatgctactt tcatatcttc
      301 attaactctt ttttctaatt cttcaagacc tctaattcca ccgataaaat caattctttt
      361 atctactcta ggattatcaa ttcctaaaat agcatcaata acatagtctt gtaatataga
      421 aatgtctaat gagtcaacag ggtcttctat ttctaataaa tttttatttg ctttcatttt
      481 ataccactta tcacctaggt acattccgaa agttcctttt ttgtcaggtt tatatttttc
      541 ttttccttct atttcttcta gttcaaaatt ttcttttatt ttatttataa attcttcctc
      601 gcttaaacca tttaagtctt tgataaccct attataatcc aaaaccatta aattctcatc
      661 tggtgcaatc attgctatga agtaattaaa ttcttctttt ccagtataat ttggattttt
      721 agctctcatt tctttggcta cattttctgc tgaagctgtc ctatgatgac catctgcaat
      781 gtatagataa tctaattttt caaatttatc tactaggtta tctattatat catcattatc
      841 aatcttccat actgtatgct ctatcttgtc atctgttatg aaatcatata aaggttcatt
      901 attatccata aaatcattga ttatatcatc tatcaccctt tgatgtttgt aagttacaag
      961 tatagttccc gtattagcat cacagtattt tatgtgttta gttctatcaa tttccttttc
     1021 tggtcgagtg tactcatgtt tttttattgt tccattcaag ctttcatcaa ctgatataca
     1081 tgctactata ccaacttgag ctctaccatc catgatttgt ttatatatat atatacactc
     1141 tttgtcatct tttactagaa tttcttgttt tcgaaactca tctaaatttt ctcgcgcttt
     1201 taaataaact ttttcatcat ataaatctat attttcatct aaatcaattt ctgatttatc
     1261 tatatgcaaa aatgaatatg gattcccttc tgcaattctt ctagcttctt tactattcat
     1321 aacatcgtaa ggaagtgctg ctactttatc tactatatat ttatttggtc taatcgcctt
     1381 aaatggttta atcgttgcca ttactaattt cctcctaatt atacaaaata ttttataatg
     1441 ctacccctat tagattgttg aagtcaagaa agaaatcttg aatcacattt actatttcgt
     1501 ctcctattct ttcttgagct tctactgtag atgccccaat atgaggtgta ggagaaactc
     1561 ttggatgatt tactaagtct aaatttaatg taggttcttg ttcataaaca tctatagcag
     1621 ctgctgataa tttaccttca tctaatgctt ttagtaaatc tttttcatca actaaaccgc
     1681 ctcttgcaca gtttattaaa taagcccctt tcttcatctt atttatttct ttttcagtta
     1741 ttactgcccc cttattctta tcaaatggta tgtgtaaaga taagaaatct gcattttcta
     1801 aaacttcttc catatcacag aatttataat tgttaaagcc ttgcgcctca cccattatgt
     1861 cagtatatat aacattcata cctaataatt ctgctctctt tgcaacttct tttgcaattc
     1921 ttccaaaacc tataagtcca agtgtctttc cattaatttc tgtaccttta tactttttct
     1981 tctcccattt tccatctctc atagttacat ttgcagtatt tatgaatctt gcaagaacaa
     2041 ataattgacc tattgtaagc tctgcaacag atatactgct tgcatttgga gtattcataa
     2101 cttttatgcc attagcttga gcatatttaa catctatatt atccagacct actccacctc
     2161 ttactatcaa cttcaacttt ttaccctcta atgctttatc tattacatct ttagtaactt
     2221 tagtagctga acgtactaca agtacatcaa catctttaag tttatctcct aaaacatctt
     2281 tttcataaaa ctgctctatc acattaaaat ctaactctct caattttctc gctgcttctt
     2341 tctctattcc atcagtaact aatatattat acataacaaa ctcctccaat ttttattcac
     2401 ttaatttttc caatttttat tttttaaagt attttcatca ataaaaaact ttacttaatt
     2461 caactttctg tttatataat tttcaaaata tttttttatt aaagtcatat caaatttttc
     2521 tattgtttgt taaaaattta atatgtaatt tttataaaat attatttatt atatattcta
     2581 aatttatctt aaaacataca tatttttaca atattattta ttaaaaccta atatatcatc
     2641 aatattatct aataattctt gtacatcttc gacagtatag tcacccatat gtgaaattct
     2701 aaaagttttt tcttttaaat ctccatagcc atttgctatt tggaatcctc gttcttgtaa
     2761 ttttgaattt aaatttgata cactaatacc ttgagtattt tctataacag taagtgtatt
     2821 ggataagtga ttttcattgg taaaaatttg gaagtgtttt ttagcccagt ttctaactaa
     2881 gtttgccatg tcttcatgtc tgttgaatct gttatctagg ccttcatcta atatattatc
     2941 taattggaaa tctaaagcga acatgtgaga aagagatggt gttgaagggt attggtagtt
     3001 tttcttgatt aagtattcat acattgctaa caaatcaaga tatactcctc taaaaggtac
     3061 ttgcttagct ctatcgattg ctttttgaga aaatgtgcaa atagacattc ctggtggtaa
     3121 tcccagtgct ttttgtactg atgttataca aatatctatt ccaatcttat caacctcgac
     3181 ttttattcca ccggctgaac ttacagtatc tacacagtag attacatctt catacttttt
     3241 aacaacttct ccaatttctt ctattggatt tcttattcca gttgaagttt cattgtgagt
     3301 tacagttatt aaatcgtatt ctccggtaga aagcacctta tcaaccattt ctggtgttgt
     3361 tgcctctcct aattcaactt taaatatatc cgctgggaca ttattagcca ctgccatttt
     3421 gtaccatcta tcaccaaagg atccacagga aaatactgct gcctttttgg atgtacagga
     3481 acgaatggag ccttccatta aaccagttcc ggatgacgtg gataaaagta tcgtgttgtt
     3541 tgtatagaat agtttttgca tcttttcact tatactcttt tggagcatag atgcatcttt
     3601 tcctctatgt cctatcattg gtgtagccat cttttgcaag acttcttctt taacatcaat
     3661 aggaccagga ataaataatt tcttactcat tataattccc ccctttttag tttgtaatat
     3721 ggatagcgtt tttactgtcc ttatttttaa taacta
//
LOCUS       DBGJFF010000225         3709 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.230, whole genome shotgun sequence.
ACCESSION   DBGJFF010000225 DBGJFF010000000
VERSION     DBGJFF010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3709
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.230"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            240..2150
                     /locus_tag="ACOIAF_003748"
     CDS             240..2150
                     /locus_tag="ACOIAF_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HGZ2670724.1"
                     /translation="MNSNTFERLQLNEVKDLIKIHCVSSLGKNLIDKLTPSGNIGVVR
                     RKLAENKEARKIIENSNHIPLEGLFNAGSTIDKIEKGMIIEPVELVNIEDFLRGCRKM
                     KAFMLEKEFYSPTLSSYALNITECKSIEDEINYCIKSNKVDSNASKELKKIRRNIEIT
                     EGKIKDRLNKFITSTVNKKYIQEFIISKRNDRYVIPIKSSYKNEVNGTILDTSSKGNT
                     VFIEPISVSNLSTELTMLKADETIEEYKILSYLTELIFEKISQIKLNIEILSEYDMVF
                     AKAKYSQKIKGITPKINNNGYIKIIKGKHPLLTGDAVPLDFEIGKNYRSLIITGPNAG
                     GKTVTLKTVGLLTLMVQCGFDISAKEGSEFSVFEKVFVDIGDNQSIENALSTFSSHIK
                     NIAEIMSLSNNSTLVLFDEIGSGTEPNEGAGLAISLLEEFYKMGCITIASTHYGEIKK
                     FATLHPEFENAGMMFDKETLEPLYKLTIGKSEDSNALFISKKMGIKNRVLDRAKEYVI
                     DRNYNLDLVKRSKLQSNIEDKNSSEVILNYTDYNVGDKVKLLDEDDFGIVYKPMDKFN
                     NVEVLFNDNFISVNIKRLQLSIKASELYPEGYDLDVLFTSFKERKLEKDIQRGSKKAL
                     KKIQKEIRENRK"
     gene            complement(2213..2836)
                     /gene="ytaF"
                     /locus_tag="ACOIAF_003749"
     CDS             complement(2213..2836)
                     /gene="ytaF"
                     /locus_tag="ACOIAF_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation membrane protein YtaF"
                     /protein_id="HGZ2670725.1"
                     /translation="MIESILLVTSLCIDAFVTSFGYGINKVKVPLYSNIIISVVCSAI
                     LAISLFAGTIVRDFLPQNITMLICFFILFLLGSARLIESFFKSYFKKKSIHSKNYNFN
                     MLDLKLNFNVSIEPDVLDTSKSKVLKSAEAFTLALALSLDGLAIGFGSGLVGVNYIQV
                     ILFSLISNIIAVLLGCLLGNMFVKNVNLNLSWLSGLILLVIGFMKIC"
     gene            3024..3668
                     /locus_tag="ACOIAF_003750"
     CDS             3024..3668
                     /locus_tag="ACOIAF_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGZ2670726.1"
                     /translation="MEQHSKVGNFGDLLKVLLDEKELSMGELSKKSGIDKSTISRIAN
                     NKQKPNINHLEKMAIHLNINLEELLKASGYEFRNSNNQIFNADSDFSNFDDILGFANL
                     INDKNFNGNIEKELSKCKLYVQTDEGKKLLFDNFNKKIDSIEKQGQFTDRLRQMYADF
                     CTDGLTIKKYLLIGSMLLYFVISTDVIPDFVFPIGFMDDLVALNIVTKLLKNDK"
BASE COUNT         1608 a          370 c          560 g         1171 t
ORIGIN      
        1 aatttatgga ataatattag aagcactgat ataagtgttt aaataagaat agaaaaggag
       61 aagtgtttta tgaaaaaaaa tactaacatt aatgagtccg gagttaaact tcttaataaa
      121 taaggagttt gatatataat aactccttat ttattaagtt gttcgcaaaa taactattat
      181 caaagtaaat gatagggtga tttaaaagac gataacttat aaataaagga gaaaaaataa
      241 tgaatagtaa tacatttgaa agattacaac taaatgaagt taaagactta ataaaaatac
      301 attgtgtaag ttcactagga aaaaatttaa tagataaatt aactcctagt gggaatattg
      361 gagtagtaag aaggaaatta gcagaaaata aagaagctag aaagataatt gaaaatagta
      421 atcacatacc acttgaagga ttgtttaatg caggttctac aatagataaa attgaaaaag
      481 gaatgataat agaacctgtt gaacttgtaa atattgaaga ttttttaaga ggttgtagga
      541 agatgaaagc ttttatgtta gaaaaagaat tttattcacc tacattaagt tcttatgcct
      601 taaatataac agaatgtaaa agtatagaag atgagataaa ttactgtata aaatctaata
      661 aagttgattc taatgctagt aaagaattaa aaaagataag acgaaatata gaaattacag
      721 aaggaaagat aaaagataga ttaaataaat ttataacatc aactgtaaat aaaaagtata
      781 tacaagagtt tataataagt aaaagaaatg atagatatgt aataccaatt aaatcttctt
      841 ataaaaatga agttaatggt actatattag atacatcatc aaaaggaaat actgtattta
      901 ttgaacctat tagtgtttca aatttaagta cagagttaac tatgctaaaa gctgatgaaa
      961 ctattgaaga atacaaaata ttatcttatt taacagaact tatatttgag aagataagtc
     1021 agataaaatt aaatatagag atactttcag aatacgatat ggtatttgca aaagctaagt
     1081 atagtcaaaa aataaaagga attactccaa aaataaataa taatggctat ataaaaataa
     1141 ttaaaggtaa acatccactt ctaacaggag atgcagtacc tttagatttt gaaataggca
     1201 aaaattatag aagtcttata ataacaggtc ctaatgcagg aggaaaaact gtaaccctta
     1261 agactgttgg tttactaact ttaatggttc aatgtggatt tgatatttct gcaaaagaag
     1321 gtagtgaatt tagtgtattt gaaaaagttt ttgtagatat aggggataat caaagtattg
     1381 aaaatgcctt aagtactttt tcatcacata taaaaaatat tgcagagatt atgagtttat
     1441 caaataattc tactcttgtt ctgtttgatg aaataggaag tggaactgaa cctaatgaag
     1501 gagctggttt agcaatatca ttattagaag agttttacaa aatgggatgt ataactatag
     1561 catcaactca ttatggagag ataaaaaagt ttgctacatt gcatccagaa tttgaaaatg
     1621 ctggtatgat gtttgataaa gaaacgttag aaccattgta taagttaact attggaaaat
     1681 ctgaagatag taatgctctt tttatttcta aaaaaatggg aataaaaaat agagttttag
     1741 atagagctaa agaatatgta attgatagaa actacaattt agatttagtt aaaagaagta
     1801 agctacaatc taatattgaa gataaaaata gttctgaagt cattttaaac tatacagatt
     1861 ataatgtagg ggataaggta aaattactag atgaagatga ttttggaatt gtatataagc
     1921 ctatggacaa gtttaataat gtagaagtat tgtttaatga taattttatt tcagtaaata
     1981 taaaaagatt acaacttagc ataaaagcat cagaattgta tccagaaggg tatgatttag
     2041 atgtattatt tactagtttt aaagaaagaa aattagaaaa agatattcaa agaggttcaa
     2101 aaaaagctct taaaaagatt cagaaagaaa ttagagagaa tagaaaataa atataaaaag
     2161 cacaaaagaa agcaatctaa agagaaaagt ttatctttag attgctttct tattaacata
     2221 tcttcataaa tccaatgact aacaagatta aaccacttaa ccaagataag tttaaattta
     2281 cgtttttaac aaacatattt cctagtaaac aaccaagtaa tactgcaatt atatttgata
     2341 ttaaagaaaa tagaataact tgtatataat tcacacctac aaggccacta ccaaaaccga
     2401 tagctaatcc atctaaactt aaagcaagtg caagtgtaaa tgcttcagca gatttaagca
     2461 ctttagattt acttgtatct aagacatcag gttcgatact tacattaaaa tttaatttaa
     2521 ggtctaacat attaaaatta taattctttg agtgtataga ttttttctta aaataagatt
     2581 taaaaaaact ttcaattagt ctagcacttc ctaataaaaa taaaataaag aaacatatta
     2641 acattgttat attttgaggt aaaaagtctc taactattgt acctgcaaaa agcgatattg
     2701 ctaaaatagc tgaacaaact acgctgataa ttatatttga atataaggga actttaactt
     2761 tatttatgcc atatccaaag cttgtcacaa aggcatctat acaaagagaa gtaactaata
     2821 aaattgattc aatcacagta gattaacatc ctttctctta aattttgtac ttatattagt
     2881 tatagtattg taaaaatgga taaattgtta cagataatcc aataaattgt tgataaaatt
     2941 ccaacatcat acaacaccta aataacaaaa gaatcatatc atataataat ataaaaccaa
     3001 tcataataaa gcgggtgaaa ataatggaac aacatagtaa ggttgggaat ttcggtgatt
     3061 tgttgaaagt tttattagat gaaaaagaat tatcaatggg agaactaagt aaaaaaagtg
     3121 gaattgataa atctactata tctcgtatag ctaataataa acaaaagcca aatataaatc
     3181 atttagaaaa gatggcaata catttaaata ttaacctaga agaactgttg aaagcttcag
     3241 gatatgaatt tagaaacagc aataatcaaa tatttaatgc agatagtgat tttagtaact
     3301 ttgatgatat tcttggattt gcaaatttaa tcaatgataa gaactttaat ggaaatattg
     3361 aaaaagagtt aagtaagtgt aagttatatg tccaaacaga tgaaggtaaa aaattacttt
     3421 ttgataattt taataaaaaa atagattcta ttgaaaaaca aggtcaattt acagatagat
     3481 taagacaaat gtatgctgat ttttgtacag atggacttac aataaagaaa tatttattga
     3541 ttggaagtat gctactttac tttgttatat caacagatgt gattccagac ttcgtgttcc
     3601 caataggatt tatggatgat ttagtagcat taaatatagt tacaaaatta cttaaaaatg
     3661 ataagtaagt tttagaatca tatttaatat ataaataaat caaagtata
//
LOCUS       DBGJFF010000226         3653 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.231, whole genome shotgun sequence.
ACCESSION   DBGJFF010000226 DBGJFF010000000
VERSION     DBGJFF010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3653
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.231"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(201..1472)
                     /gene="serS"
                     /locus_tag="ACOIAF_003751"
     CDS             complement(201..1472)
                     /gene="serS"
                     /locus_tag="ACOIAF_003751"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006441208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HGZ2670727.1"
                     /translation="MLDIKRIRENLDDIKKAMERRGEREFDLDAVVELDNKRREILQE
                     VEVMKNELNVNSKKIPQLIKEGKDVTEEKARLKELSDKIKGIDEKVKEVEAKMEYTLM
                     RIPNVPHPEVPQGETDEDNVQIRTWGEPTKFDFEHKAHWDIGTGLGILDFERAGKITG
                     SRFTLYRGLGARLERSLMNFFLNTHTAKHGYTEVLPPFMANRNSFIGTGQLPKFEEDM
                     FKIEGLEYFLIPTAEVPVTNIHANEILDVAELPIKYCAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVELVKFVKPEDSYNELESLTHDAETMLQMLGLPYRVVKICTGDLGFTAAFK
                     YDLEVWMPSYNRYVEISSCSNFEDFQARRAGIRFKRDKKSKAEYVHTLNGSGLAIGRC
                     LAAILENYQQADGSVVVPEELRPYMGVDVIK"
     misc_binding    complement(1598..1803)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1931..2812)
                     /locus_tag="ACOIAF_003752"
     CDS             complement(1931..2812)
                     /locus_tag="ACOIAF_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HGZ2670728.1"
                     /translation="MNFLSLAKTASTNSNITKRTLENMLDNFSDNIPNIIYAIIVFVI
                     GIYISKIVRRMVSKFLIKYGMSKGVNNFIVYGIYISMLSIISLISLGIIGIQTTSVVA
                     VLGAAGFSIGLAFKEILSNLGSGMIILFFKPFNIGDYIQGSGVEGTVSDIQIFSTVLK
                     TPDNKTIIIPNFQLTSNNIINYTHQNKRRIDFSYNISYDSDIDVVKSILNEIFTNEKR
                     ILNDPKPIIGLNSIGNNTMQIVARPWVKTDDYWDVYFDVMEKVKNKFDENDIKVPFVP
                     SSLLFNNTGNLNTMDKR"
     gene            complement(2824..3333)
                     /locus_tag="ACOIAF_003753"
     CDS             complement(2824..3333)
                     /locus_tag="ACOIAF_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HGZ2670729.1"
                     /translation="MNSNERRSKLIDILKESKHPVKGGTLAELLNVSRQVIVQDIALI
                     RARGFEIIATPQGYIIYNQPYFTKQIKCKNHKDSKEIYDELKIIVDLGGIIKDVIVNH
                     PTYGQITAELNIYSKMDIDNFMKKVETNEFKQLSVLTECSHIHTIEAIKEETIEAIVK
                     ELKDNGILS"
     gene            complement(3460..3576)
                     /gene="rrf"
                     /locus_tag="ACOIAF_003754"
     rRNA            complement(3460..3576)
                     /gene="rrf"
                     /locus_tag="ACOIAF_003754"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1173 a          636 c          363 g         1481 t
ORIGIN      
        1 taaaataatt tttagatatt catacaagaa tgttttataa aaatatttag tgttttatga
       61 tgaaaattct gctgtgtaaa ttaacataaa aaaactatga aattaatatt tataataatt
      121 tcatagtttt tttatttatt gtttactcat aatatattct cttcatcaaa gcatactcta
      181 catatacaat taatataaat ttattttata acatcaacac ccatataagg tcttaattct
      241 tctggaacta ctacagaacc atctgcctgc tgataatttt ctaatatagc agccaaacat
      301 cttcctatcg ctaatccaga accatttaat gtatgaacat attctgcttt tgatttttta
      361 tctcttttaa atcttattcc tgctcttctt gcttggaaat cttcaaaatt tgaacaagaa
      421 gatatttcta catatctatt gtaacttggc atccaaactt ctaaatcata tttaaatgca
      481 gctgtaaatc ccaaatctcc tgtacatatt tttacaactc tatatggaag acctaacatt
      541 tgtaacatag tttcagcatc atgagttaat gattctaatt cattataaga atcttctggt
      601 ttaacaaatt taactaattc aactttatta aattgatgtt gtctaactaa acctcttgta
      661 tctcttcctg ctgaacctgc ttcagatcta aaacaaggag tatatgcaca gtatttaatt
      721 ggaagctctg caacatctaa tatttcatta gcatgtatat tagtaacagg aacttcagct
      781 gttggtatta agaaatactc taatccttct attttaaaca tatcttcttc aaatttagga
      841 agttgaccag tccctataaa actatttcta ttggccataa atggaggtaa gacttccgta
      901 tagccatgct tagctgtatg agtatttaaa aagaaattca ttaaagatct ttctaatcta
      961 gctcctagtc ccctatataa agtaaacctt gacccagtta ttttccctgc tctttcaaaa
     1021 tctaaaatac ctagacctgt acctatatcc caatgcgctt tatgctcaaa atcaaattta
     1081 gtaggttctc cccaagttct tatttgaaca ttgtcttcat ctgtttcacc ttgtggtacc
     1141 tctggatgag gaacatttgg tattctcatt aatgtatatt ccatttttgc ttctacttct
     1201 tttactttct catcaatacc ttttatctta tctgataact ctttcaatct tgccttttct
     1261 tctgttacat cttttccttc ttttattaat tgaggtattt ttttagaatt tacatttaac
     1321 tcatttttca taacttcaac ttcttgaagt atttctcttc ttttattatc taattccact
     1381 acagcatcaa ggtcaaattc tctttcacct cttctttcca tagctttttt gatatcatct
     1441 agattttctc ttattctttt aatatctaac atattaattc ctcctaataa ttttgataat
     1501 ataagttata attccaactt aatttacaaa cattatactt gtaaactctt agtttaaaca
     1561 aattttaaaa ataaaaaaag acctccgttt accctataaa agggacgaaa atcttttatc
     1621 cgcgttgcca ccctaattga ctatactaaa atgtataatc ctctcagacc tataacgtag
     1681 gtaaacgtct agctttaact agaagctcta gagtagattc aaaaaccatt gtaatgagtt
     1741 ttcaccatcc actcattctc tgaatacaat ctgtttttta ctattttcct tcacagcttt
     1801 ttaactaatt gttttatttg ataatcatta tattatatat attaacatct ttcaatacta
     1861 ttattcaaat ttttttagaa ttttctttaa ttttttatat taaattcatt ttttctattt
     1921 aattaagacc ttatctttta tccattgtat ttagattacc tgtattatta aataataatg
     1981 aagaaggaac aaatggaact tttatatcat tctcatcaaa tttatttttt accttttcca
     2041 ttacatcaaa atatacatcc cagtaatcat cagtttttac ccaaggtcta gcaactattt
     2101 gcatggtgtt atttcctata gagtttaaac ctattatagg ctttggatca tttaatattc
     2161 ttttttcgtt tgtaaaaatt tcatttaata tagacttaac tacatctata tcactatcat
     2221 atgaaatatt atatgaaaaa tctattcttc ttttgttttg atgtgtgtaa ttaattatat
     2281 tattacttgt cagttgaaaa ttaggtataa ttatagtttt attatctggt gtcttaagaa
     2341 cagtgctaaa tatttgtatg tcagaaactg taccttctac acctgaacct tgaatataat
     2401 cacctatatt aaatggctta aaaaataaaa taatcatacc agagcctaaa tttgataata
     2461 tttctttaaa agctaaacct atactaaatc cagcagctcc taacacagct acaactgatg
     2521 tagtttgtat ccctataata cctaagctta tcaatgatat aatagataac atagatatat
     2581 atattccata tacaataaaa ttatttacac ctttactcat tccatatttg attagaaact
     2641 ttgaaaccat ccttcttact attttagata tataaatacc tatcacaaat acaattattg
     2701 catatataat atttggtata ttatcactaa aattatcgag catattttct aaagttctct
     2761 tagttatatt gctatttgtt gaggcagtct tagccaatga taaaaaattc attcaactcc
     2821 tccttatgat aatattccgt tgtcctttaa ctcttttact atagcttcta tagtttcttc
     2881 ttttattgcc tcaattgtat gaatatggct acattctgtc agaaccgata attgtttaaa
     2941 ttcatttgtc tcaactttct tcataaagtt gtctatatcc attttagaat aaatattaag
     3001 ttctgcagtt atctgaccat atgttggatg atttacgatg acatctttta taataccacc
     3061 taaatccaca attatcttta attcatcata aatttcttta gaatccttat gatttttaca
     3121 ctttatttgt tttgtaaaat acggttgatt atatattata tatccttgag gtgtagctat
     3181 tatttcaaat cctctagctc ttataagggc tatatcttga actattactt gcctacttac
     3241 atttaatagt tcagctagtg ttccaccttt tacaggatgc ttagattctt ttaaaatatc
     3301 tattaattta cttcttcttt cattagaatt catattacac cttctttcca aataaaaact
     3361 ttacctatac tatataattt tatattttat cttattataa atcaatattg actttatatt
     3421 ctatttaatc ttaactttta taataaataa aaaaagacta cgtggctacg tcctactctc
     3481 ccaggcagtt tcccaccaag taccatcagc gctagagagc ttaacttctg tgttcggaat
     3541 gggaacaggt gtatcctctc tgctattgta accacataat ctttatgctt aatatttcta
     3601 ttaagttttt aataagttta tagaacctag caatactagt ttctttagag tta
//
LOCUS       DBGJFF010000227         3591 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.232, whole genome shotgun sequence.
ACCESSION   DBGJFF010000227 DBGJFF010000000
VERSION     DBGJFF010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3591
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.232"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            137..1336
                     /locus_tag="ACOIAF_003755"
     CDS             137..1336
                     /locus_tag="ACOIAF_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670730.1"
                     /translation="MKLKSKISIFIIFLTLAIYFSIGLLNNEDNLKIKTIKGDSNELD
                     HININFGYALNGYINSYYTINGSNKTKKTELIKLQKDSKNTNNLIDVSSGSDLIAHVG
                     FEMNEESKDINFVIDEKKEDLEKGVFPSEGSDKEIEYEKLDMNNFTRTRYKVDKSTDD
                     YYAKILASKRYKKYLYVSVLYATSDSSENNKEYSSTIDIIKINPENKKIKTIKSINLS
                     NYFSEKYQNIENIGVSYASKYEDKMYILVNVLNTNIGDNSNSKFHLLEYNLEENKESI
                     MELKTDSKMALAKAKLEANKLYMVMSNDYDNSSITELNLLTYNILDKSLDEDKYVLDN
                     REKRGIRSIEIDSNKIVAYTSRLNSILSWDDKFMLYIINKNSKEIVYEGELQFNSSNS
                     MLEFEIK"
     gene            1345..2157
                     /locus_tag="ACOIAF_003756"
     CDS             1345..2157
                     /locus_tag="ACOIAF_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670731.1"
                     /translation="MKDLIRLFEFELKRNIKNYIFIIICCCSFVILNIVKNLNDYNYI
                     IENAVKSEQLTKIGNTIETVNVTGFSSFRNIMGSTESWFMFGIIACICYAFFIWYRDF
                     NGRSKSIYTLIMLPKNRINIYISKLLNILFLVYSYTVVLTISLFIASKLLPRHMLGNV
                     TNYGFVQETIYELKMLLPYSFEILFVEYIFLLIGFVSVVFTSILINKSIYKVSTLISF
                     LFLVIEILVFIISIEFIIPYEYSDVFTVLYSSINIFVCSLISNKLLKQKIDF"
     gene            2168..3436
                     /locus_tag="ACOIAF_003757"
     CDS             2168..3436
                     /locus_tag="ACOIAF_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670732.1"
                     /translation="MKAKNIGKYTICFIIVCFLMITMTLILSYFNKDKLSVKTLKGNV
                     KDIGRVSVVYTPDISKFSYEEVIISKNGVRNNTNPKSRIRNDYLNIDKKYSDFVNDKG
                     KEFIYKDDLNIGEAYTSVKSDLVQGMNALNDCIKVSNKNLKSGVEENFDINLNKSDFK
                     ENSWKTLFNKIYKGNLYMVISETNISGSKVNNICILKIDIKNKSYKLLNRFNLGLQNG
                     EQQNINEIKFSIGSKLYLEIVNNKSKNGYIEGHDSYSNFIIYDIEDNTFNISNKFMDN
                     LNKEKNANPKEIGLLSKTKLDYMVKNNKLNLIVTDMSNSITNLVYDINSNEIKLEDYE
                     ILDLKVDYNEASSGVRKVKLLDDKIYSISGIKNSISGELKGFKGVVDVVGTKPVKFQV
                     FDMKLKKNIYEAELINGDISLQEKLFFVAN"
BASE COUNT         1526 a          275 c          542 g         1248 t
ORIGIN      
        1 aatattataa tcttatagga tttcaattta acaatttagt agcagggtta ttaagttttt
       61 taattataat gtttattcta aatggaatat cttatttgct aattaaaaag aaagtatctc
      121 tttaaggagg ggtggtatga aattaaaatc taaaatatct atatttatta tatttttgac
      181 tttagcaata tattttagta taggtttatt aaataatgaa gataatttaa agataaaaac
      241 tataaaaggt gactctaatg aattagacca tataaacata aattttggat atgctttgaa
      301 tggatatata aattcatatt atacaataaa tggaagtaac aaaaccaaaa agactgaatt
      361 aataaaacta caaaaggata gtaagaacac aaataattta attgatgtat ctagtggttc
      421 agatttaatt gctcatgttg gatttgaaat gaatgaggag tcaaaagata taaactttgt
      481 gatagatgag aaaaaagaag atttagaaaa aggtgtattt ccatcagaag gtagtgataa
      541 agaaattgaa tatgaaaaat tagatatgaa taattttaca aggacaagat ataaagtaga
      601 taaaagtaca gatgattatt atgcaaagat actagcatca aaaagatata aaaaatactt
      661 atatgtatct gtactttatg ctacaagtga ttcatcagaa aataacaaag aatattcaag
      721 tactatagat ataataaaaa ttaatccaga aaataaaaaa atcaagacta taaaaagtat
      781 aaatttaagt aattattttt ccgaaaaata ccaaaacata gagaacatag gtgtaagtta
      841 tgctagcaaa tatgaggata agatgtatat tttagtaaat gtactaaata caaacatagg
      901 tgataatagt aattctaaat ttcatttact tgagtataat cttgaagaaa ataaggaaag
      961 cataatggaa ttaaaaactg atagcaaaat ggctttagca aaagcaaagt tagaagctaa
     1021 taaattatat atggtaatgt cgaatgatta tgataatagt tcaattacag agttaaattt
     1081 acttacctat aatatattag ataaaagctt agatgaagat aaatatgttt tagataatag
     1141 agaaaaaaga ggaattagaa gtattgaaat tgattcaaat aaaatcgttg catatacatc
     1201 aaggctaaac tctatattat cttgggatga taaatttatg ctatatataa taaataaaaa
     1261 ttctaaggaa attgtatatg aaggagaatt acaatttaac agttcaaata gtatgttaga
     1321 atttgaaata aagtaggtga gttgatgaaa gatttaataa gattattcga atttgaatta
     1381 aaaagaaata taaagaatta tatatttata ataatttgtt gttgttcttt tgtaatttta
     1441 aacatagtaa aaaatttaaa tgactataat tacataatag aaaatgcagt taaaagtgaa
     1501 cagttaacaa aaatcggaaa tacgatagaa acggtaaatg ttacaggttt ctcaagcttt
     1561 agaaatataa tgggttctac agaatcgtgg tttatgtttg gtataattgc atgtatttgc
     1621 tatgcttttt ttatttggta tagagacttt aatggtagaa gtaaatctat atatacactt
     1681 ataatgctac ctaaaaatag aatcaatatt tatatatcta aattattaaa tatattattt
     1741 ctagtgtata gctatacagt agtacttaca atatctttgt ttattgcaag taagttgttg
     1801 cctagacata tgcttggtaa tgtcacaaac tatggttttg tacaagaaac aatatatgag
     1861 ttaaaaatgt tacttccata tagctttgaa atactatttg tagagtatat atttttattg
     1921 ataggatttg tatctgtagt atttacatca attcttataa ataaatctat ttataaagta
     1981 agcactttaa taagttttct atttttagtt atagaaattt tagtgtttat aatttctata
     2041 gaatttatta taccttacga atacagtgat gtatttactg ttctatattc aagtataaac
     2101 atatttgtat gtagtcttat atctaataaa cttttgaagc aaaagattga cttttaggag
     2161 ggatattatg aaagcaaaaa atataggaaa atatacaata tgttttataa tagtatgttt
     2221 tttaatgatt actatgacct taatattgag ttattttaat aaggataaac tatctgtaaa
     2281 aactttaaaa ggcaatgtaa aagatatagg gagagtaagt gtagtatata cacctgatat
     2341 aagcaaattt tcttatgagg aggtaattat aagtaaaaat ggagtaagaa ataatacaaa
     2401 cccaaaatca agaattagaa atgattattt aaatattgac aaaaaataca gtgactttgt
     2461 taatgataaa ggaaaagaat ttatatataa agatgatttg aatataggag aagcgtatac
     2521 ctctgttaag tctgatttag tacaaggaat gaatgcttta aatgattgta taaaggtttc
     2581 aaataaaaat ttaaaaagtg gggtagaaga aaattttgat attaatttaa ataagtctga
     2641 ttttaaagaa aatagttgga aaaccttatt taacaaaata tataaaggta atttatatat
     2701 ggtaattagt gaaactaata tttcaggttc taaagtcaat aatatttgta tattaaagat
     2761 agatataaaa aataagtctt ataaattatt aaatagattt aatttaggat tacagaatgg
     2821 agaacaacaa aatatcaatg aaattaaatt ttctattgga agtaaattat acttagaaat
     2881 agtaaataat aaaagtaaaa atggatatat tgaaggacat gatagttact ctaactttat
     2941 tatatatgat atagaagata acacttttaa tataagtaat aaatttatgg ataatctaaa
     3001 caaagaaaag aatgcaaatc caaaggagat tggcttatta agtaaaacta agctagatta
     3061 tatggtaaaa aataataaat tgaatcttat agttacggat atgagtaatt ccataactaa
     3121 tcttgtttat gatattaatt ctaatgagat taaattagaa gattatgaga tacttgattt
     3181 aaaagtagat tataatgaag catcatctgg agttaggaaa gttaagctac tggatgataa
     3241 gatatattct atttcaggta ttaagaacag tatatcagga gaattaaaag gatttaaagg
     3301 ggttgttgat gtagtcggaa ctaagccagt aaaatttcaa gtatttgata tgaaacttaa
     3361 aaagaatatt tatgaagctg agctaataaa tggagatata agtttacaag aaaagttatt
     3421 ttttgttgca aattaagtgt tagaattaat tttagaactt taaactttta tatgtatcac
     3481 agataagtat aagtaaggga gtgtctcaaa ataaactttt tagctcatga gacacttttt
     3541 ttatatgaaa tcaaatatat tttcattgtt tagatttatt ttgataatga a
//
LOCUS       DBGJFF010000228         3565 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.233, whole genome shotgun sequence.
ACCESSION   DBGJFF010000228 DBGJFF010000000
VERSION     DBGJFF010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3565
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.233"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            <1..227
                     /locus_tag="ACOIAF_003758"
     CDS             <1..227
                     /locus_tag="ACOIAF_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2670733.1"
                     /translation="KDTTAIIVGSFIYQFTIYFAMSLGMLSTDLKLITAIVIIAFLAT
                     GNLNISLKKNKCKASTKNKSEKRGVVDVTN"
     gene            214..969
                     /locus_tag="ACOIAF_003759"
     CDS             214..969
                     /locus_tag="ACOIAF_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2670734.1"
                     /translation="MLQIKNLSKSFPNPYGEPNTIFENLSIDIEDGEFVSIIGSNGTG
                     KSTLLNIISGLIKESSGQITLDSTTITNLAEHRRTQIVSRVFQDPSLGTCPSMTVREN
                     LSLALNKGKLLNLKKCLRHKTNDLEHLLEGISLDLKKYLDIKVQYLSGGQRQSLSLIM
                     SSLASPKVLLLDEHTAALDPKTSNEVIELTDKIVREKNITTLMVTHNLKHALQYGDRL
                     IMLHKGEVVLDVKDKEKEDLTVEEILEKFEYAV"
     gene            1066..2103
                     /locus_tag="ACOIAF_003760"
     CDS             1066..2103
                     /locus_tag="ACOIAF_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGZ2670735.1"
                     /translation="MIKSKKILSLIIAGVLGVSMLTGCSQNDGSNASNENKETDSKKQ
                     KNIGISQLVEHPSLDKAKKGFIKALEDKGYKDGDNIKIDFQNAQNDMPTTQSIASKFV
                     SDKKDLIYAISTPSAQAAYNATKDIPIIMTAVTDPVEAGLVKSLEKPGGNVSGTSDYL
                     SIDKTLELVKTLTPKAKKIGVIYNTSEVNSKIQVDSLHDYAKKNNYEVVEKGISSSSE
                     VNQAISSLVGKIDVLYVPTDNLIVSSMPIVSKVANENKIPIIASEEGSVSSGALACCG
                     IDYEKLGYKAGELAIEVLEGKSVGDIPVTTLDETEIIINEDTLKALDMQKLSADNIKY
                     IKSDENAKSAK"
     gene            2912..>3565
                     /locus_tag="ACOIAF_003761"
     CDS             2912..>3565
                     /locus_tag="ACOIAF_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2670736.1"
                     /translation="MSGIISVMTQSLILSIMALGVYITYKILDFPDMSADGSYTMGAS
                     IVAFSLTNGNSPVVATLMAILCGCTAGLVTGILHIKFKISNLLSGILVMGMLYSINLR
                     IMGKSNIPLFSFKHLFNGEISPIVLALAFVFICKVLLDLFLKTGLGYTLKGVGDNSQM
                     IKSLGINIGSIKILGLMISNGLIALSGSLMAQFLGFSDVNMGIGTLVLGIASIIIGIT
                     "
BASE COUNT         1331 a          475 c          548 g         1211 t
ORIGIN      
        1 tcaaagatac tactgcaata attgttggct cttttatata ccaattcact atatactttg
       61 caatgagttt aggaatgtta tcaactgact taaaactaat aactgccata gttataatag
      121 ctttcctagc tactggtaac ttaaatattt cactaaaaaa aaacaaatgc aaagctagta
      181 ccaaaaataa atcagaaaaa agaggtgttg tagatgttac aaattaaaaa tctttcaaag
      241 agtttcccta atccatatgg tgaacctaat actatctttg aaaatttatc aatcgatata
      301 gaagatggtg aatttgtaag tatcataggt agtaatggaa ctggtaaatc tactttatta
      361 aatataatat ctgggcttat aaaagaatct tctggtcaaa ttactttaga tagtactact
      421 ataacaaatt tagctgagca cagaagaact caaatagtaa gtagagtttt tcaagaccca
      481 agccttggaa cttgtccttc tatgacggtt agagaaaatt tatctctagc acttaataaa
      541 gggaaattat tgaacttaaa aaaatgtctt cgtcataaaa cgaatgactt ggaacactta
      601 cttgaaggaa tttctttgga tttaaaaaaa tatttagata ttaaagtaca atatctttca
      661 ggaggtcaaa gacaatcact atcactaatt atgtctagct tagcaagtcc taaggtctta
      721 ttactagacg aacatacagc agcacttgac ccaaaaacat caaatgaagt aatagagtta
      781 acagataaaa tcgtaagaga aaaaaatata actacactta tggttactca caacttgaaa
      841 cacgctcttc aatatggaga taggttgata atgcttcata agggtgaagt tgtattagat
      901 gtaaaagata aagaaaaaga agatttaaca gttgaagaaa tcttagaaaa atttgaatat
      961 gcagtataag aaattaaaat cttagtttta atcagaaata tttataatat atcatatgaa
     1021 tatatgaaca aatataatca ataaatttta agggaggaaa ataaaatgat aaaaagtaaa
     1081 aaaatattaa gtttaattat agcaggagta cttggtgtat caatgcttac tggatgttct
     1141 caaaatgatg gctccaatgc ttcaaatgaa aataaagaaa cagatagcaa aaaacaaaaa
     1201 aatataggta ttagtcaatt agtggaacat ccgtctctgg ataaagcaaa gaaaggattc
     1261 atcaaagcac tcgaagataa aggctataaa gatggagata atataaaaat agatttccaa
     1321 aatgcacaaa atgatatgcc tactacacaa agtattgcta gtaagtttgt atccgacaaa
     1381 aaagatttga tatatgctat atctactcca tctgcacaag cagcttataa tgctactaaa
     1441 gatattccga taataatgac tgctgttaca gaccctgtag aagctggact tgttaaatct
     1501 cttgaaaaac caggtggaaa tgtttctggt acatctgatt atctttcaat tgataaaaca
     1561 ttagaattgg ttaaaacatt gactccaaaa gcaaagaaaa taggggttat atacaatact
     1621 agtgaagtta attcaaaaat ccaagttgat tctctacatg attatgctaa gaaaaataat
     1681 tatgaagtag ttgaaaaagg aatcagctct tcaagtgaag ttaaccaagc tatttctagt
     1741 ttagttggca aaatagatgt tttatatgtc cctactgaca atttaatagt ttcttctatg
     1801 ccaatagttt ctaaagttgc taatgaaaac aagattccta taatagcctc tgaagaaggt
     1861 tctgtatctt ctggtgcttt agcttgttgt ggaatagatt atgaaaaact aggttataag
     1921 gctggagaac ttgctattga agtattagaa ggaaaatctg ttggcgatat accagttact
     1981 acattagatg aaactgaaat aataattaac gaagatacac taaaagcact tgacatgcaa
     2041 aagttatcag cagataatat aaagtatata aagtcagatg aaaatgcaaa atctgcaaaa
     2101 taaaacttat taatttcaaa aaataggtaa tatgaagtag gtaatatgaa aactttatat
     2161 aataagtact cttatccacc ctaacccttt gaaaattgtg cttattgtag caatttgaat
     2221 attgcatata aagttattca ttttccataa aagtcgtcta tattgacgac tgattatgaa
     2281 agttatataa ctttctaatc actacttgag ctgtccaaaa gaattttaat attctccaga
     2341 cagctttttt ttatgatatt tgattgatat acatgtctag ttattccata tctagtaaat
     2401 attttgataa aatttccaca ttcacttata acttaggata acgttttttg caaataatac
     2461 attaatttaa aagtattctg agtatacatt atttagtatt ttaattaaat ttagcagttg
     2521 acttttatta ttgtataatt tataatttat aacaagtcga gaatacatat aattttctga
     2581 ctaattaaaa tattaaaagg gagagataat tatgaatact ttattaaaca atagaaaata
     2641 ttattattat tatagccact atttttaggg tttgtaccta cgttttaaca tagatacaga
     2701 ccccttaaga ctactaaaat tttgggattg tatctatgtt ggctatattg tttataaagt
     2761 tttcatttaa ctatataaga gccacataga ttaaactatg tggcttattc gtgtttttac
     2821 cacatagttt tgtacactat gtggtttttt tatacacaaa attagcaaat aattaaaatt
     2881 ataaaaatat tttcaaagga gtgttttatt tatgtcaggt attatatctg taatgacgca
     2941 aagtttaatt ttatcgatta tggcactggg ggtttatata acttacaaaa tcttagattt
     3001 tccagatatg tctgctgatg gtagctacac aatgggtgct tctattgtag cattttctct
     3061 tactaatggt aattctcctg tagtagcaac tttaatggct atcttatgtg gttgtactgc
     3121 tggtcttgtt acagggattc ttcacattaa gtttaagata tctaacttac tttctggtat
     3181 tttagttatg ggaatgctat actctatcaa tttaagaata atgggaaaat caaatattcc
     3241 tctatttagc tttaaacact tattcaatgg ggaaatatct cctatagttt tggccttagc
     3301 ttttgtattc atctgtaaag tccttttaga cttattccta aaaacaggtc ttggatacac
     3361 tttaaaaggt gttggtgata actctcaaat gattaaatcc ctaggtataa atatcggttc
     3421 tataaaaata ctaggactta tgatttcaaa cggacttata gctttatctg gtagtcttat
     3481 ggctcagttc ttgggattct cagatgtaaa tatgggtata ggaactttag ttcttggaat
     3541 tgcctctatc ataattggta ttaca
//
LOCUS       DBGJFF010000229         3557 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.234, whole genome shotgun sequence.
ACCESSION   DBGJFF010000229 DBGJFF010000000
VERSION     DBGJFF010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3557
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.234"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            150..884
                     /locus_tag="ACOIAF_003762"
     CDS             150..884
                     /locus_tag="ACOIAF_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGZ2670737.1"
                     /translation="MKIVNKSSNIPLHTQLSSIIREMIETGELKEGDAIMPERELCNI
                     QNVSRMTVNKTIVGLVTEGLLYRVQGKGTFVAKQKKKYQFSNVKGFTDVMKEKGVNIK
                     TDILSFEMELPDDLVKRKLGISDNTTNIYKIVRLRYTDGEPFGLEIVYLSEEMCKGLT
                     KGILDNSSLYRVLNEKYGYKIQKAEQVMEPVILSDEESKLLETDEGALALKLHRNSYN
                     REGSPIEYTISIFRTDKYQYEIVLSE"
     gene            894..2027
                     /gene="nagA"
                     /locus_tag="ACOIAF_003763"
     CDS             894..2027
                     /gene="nagA"
                     /locus_tag="ACOIAF_003763"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HGZ2670738.1"
                     /translation="MKCLINGKIILKNQILENKVLIFDEKIIDIADSVPKDCEVIDAD
                     GKYISPGLIDIHIHGNMGKDTMDSTDESIETISKSIMRHGVTSFLPTTMTMDKEHVYD
                     ALEVIKKAQNRKLEGAQVLGAHLEGPFINENYKGAQNEKFIINSKYEFIKEYKDVIKV
                     ITYAPEKDIDFDFTREIKRCTDIVLSIGHSNANYDQAKEAINLGVTNVTHMFNAMTGL
                     NHRDPGVVGAALTTNVYSELIADTIHINKDLFQFILNNKGKERLILITDSIEAGGLED
                     GNYSLGGQKVIVKGNEARLENGALAGSVLSLNKMVFNFLDNTNLKVNEAINLASLNPA
                     TSLGINDKKGSLEIGKDADIAVFDENLDCKMTLCLGDVVYKNI"
     gene            2126..2875
                     /gene="nagB"
                     /locus_tag="ACOIAF_003764"
     CDS             2126..2875
                     /gene="nagB"
                     /locus_tag="ACOIAF_003764"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HGZ2670739.1"
                     /translation="MRVLVCKDYDGMSKKAAEMIAAQIVLKPNSILGLATGSTPVGMY
                     RDLVKKYNDNIVDFSDVMSFNLDEYYKLPISNDQSYDYFMKENLFNHVNIKPENTHLP
                     NGMADDIEKECMNYEASIDAAGGIDVQVLGIGRNAHIGFNEPDTKFAKRTHVVELTES
                     TIEANARFFKSREDVPKKAVSMGIGSILKSKKILLLASGEEKADAVYNTVYGDITPEV
                     PGSILQLHKDTIVIVDEAAASKLNPKDYKLV"
     gene            3050..3322
                     /locus_tag="ACOIAF_003765"
     CDS             3050..3322
                     /locus_tag="ACOIAF_003765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670740.1"
                     /translation="MLKIIMFSGILSSIIKLPIIGILPTGNIKNNTGISDSKKDGKQR
                     RSNLGISIYQLPSFLYSLINNSVPSNKPIPPEEIKLIKMYLLKSCI"
BASE COUNT         1461 a          382 c          607 g         1107 t
ORIGIN      
        1 tatagtataa actccaattt cttttttgtc tttaggtttt attaaaattt ttacaaaata
       61 agttaaaaag tattgaaata aaatttcttt taaagtataa ttggtatata ccaaaattgg
      121 tatataccat atcgaaaagt ggtgactata tgaaaatagt aaataaatct agtaatatac
      181 cactgcatac tcaattaagt tcaataataa gagagatgat agaaactggt gagcttaaag
      241 agggtgatgc aataatgcca gaaagagaac tttgcaacat acaaaatgta agtcgtatga
      301 cagtaaataa aaccattgtt ggattagtta cagaaggact tctttatagg gtacaaggta
      361 aagggacatt cgttgcaaag caaaaaaaga agtatcagtt ttcaaatgta aaaggtttta
      421 ctgatgttat gaaagaaaag ggagtaaaca taaaaacgga tatattatcc tttgagatgg
      481 aactaccaga tgatttagtt aaaagaaagc ttggaataag tgataatact acaaatatat
      541 ataagattgt cagacttaga tatactgatg gagagccatt tggattagaa atagtatatc
      601 tatcagaaga aatgtgtaag ggtctaacaa aagggatatt ggataattct tcattatata
      661 gggttttaaa tgaaaaatat ggctataaga tacaaaaagc tgaacaggta atggagccag
      721 taatactttc agatgaagaa agtaagcttc ttgaaacaga tgaaggtgct ttagctctta
      781 aacttcatag aaattcctat aatagagaag gtagccctat agaatatacc atatcaatat
      841 ttagaacaga taaatatcaa tatgaaatag tattgagtga gtaggtgaat aatatgaaat
      901 gtttaattaa tggaaagata atattaaaaa atcaaatctt agaaaataaa gtattaatat
      961 ttgatgaaaa aataattgat atagctgata gtgtaccaaa agattgtgaa gttatagatg
     1021 ctgatggaaa atatatatct ccagggctta ttgatattca tatacatgga aacatgggaa
     1081 aagatacaat ggattcgact gatgaatcta ttgaaacaat ttcaaagtca attatgagac
     1141 atggagtaac atcttttctg ccaactacaa tgactatgga taaagaacat gtttatgatg
     1201 cacttgaagt tattaaaaaa gcccaaaata gaaagcttga aggggcacaa gtattaggag
     1261 cacatttaga aggacctttt ataaatgaga attataaggg agcacaaaat gaaaaattta
     1321 taataaattc aaaatacgaa tttataaaag aatataaaga tgttataaaa gttataactt
     1381 atgctccaga aaaagatatt gattttgatt ttactagaga aataaaaaga tgtacagata
     1441 tagttttatc tattggtcat agtaatgcaa attatgacca agctaaagaa gctataaatt
     1501 taggcgtaac taatgtaact cacatgttca atgcaatgac tggtttaaac catagagacc
     1561 caggcgttgt tggtgcagca cttacaacta atgtgtacag tgagttaata gcagatacaa
     1621 tccatataaa taaagattta tttcaattta tactaaataa taaaggaaaa gaaagactga
     1681 ttttaataac agattctata gaagctggtg gtcttgaaga tggcaattat tctcttggag
     1741 gtcagaaagt catagttaag ggtaatgaag ctagacttga aaatggagca ttagctggaa
     1801 gtgtgttgtc tttaaataaa atggtattta acttcttaga taatacaaac cttaaagtga
     1861 atgaagcaat taatttagct tctttaaatc cagcaacatc tttgggaata aatgacaaaa
     1921 aaggaagctt agaaataggt aaggatgcag atatagctgt atttgatgag aatttagatt
     1981 gtaaaatgac actttgcttg ggggacgtag tatataaaaa tatatagtat acaaaacaaa
     2041 ccttaaagac agtaaaaaca atataaaaaa taataaatta aacttacatt agtaaaagta
     2101 gtagttaaaa tttaggagga ataagatgag agtattagtg tgtaaagatt atgatggaat
     2161 gagtaaaaaa gccgctgaaa tgatagccgc tcaaatagtt ttaaaaccaa attcaatact
     2221 gggactagct acaggaagta caccagtagg tatgtataga gatttagtaa aaaaatacaa
     2281 tgataatata gtagattttt cagatgtgat gagctttaat ttagatgaat attataagtt
     2341 accaatatct aatgaccaaa gctatgatta ttttatgaaa gagaatctat ttaatcatgt
     2401 aaacataaag ccagaaaata ctcatctacc aaatggaatg gctgatgata ttgaaaaaga
     2461 gtgtatgaac tatgaagctt ctatagatgc agctggtggt atagatgtac aagtacttgg
     2521 aataggtcgt aatgctcata taggttttaa tgaacctgat actaaatttg caaaaagaac
     2581 tcatgtagtt gaacttacag agtctacaat agaagctaat gcaagattct ttaaatcaag
     2641 agaagatgtt cctaaaaaag ctgtaagtat gggaataggt tcaatattga agtctaagaa
     2701 aatattgctt ttagcttcag gagaagaaaa agctgatgca gtatataata ctgtttatgg
     2761 agatataact ccagaagttc ctggttctat acttcaactt cataaagaca ctatagttat
     2821 agtagatgaa gcagcagcaa gtaaattgaa tccaaaagat tataaattag tataagaata
     2881 gaatgtagtt taagtaaaaa atagctatca ttgaataatc aaatttattt aatgatagct
     2941 atttttattt aaatttttta cttcttttta tttaaattag ttattattgt ttaaattact
     3001 tgcttattac tttttacgaa aactcgttat caagaagtat ccgaataaga tgttaaaaat
     3061 tattatgttt agtgggattc tatcaagcat aataaaatta cctataattg ggatattacc
     3121 aactggaaac ataaagaata atacaggcat cagtgatagt aaaaaagatg gaaaacaaag
     3181 aagaagtaat cttggaatat caatatacca actaccatcc tttttgtatt ctctaatcaa
     3241 taattcagtt cctagtaata aaccaatacc accagaggaa attaaattaa taaaaatgta
     3301 tcttttaaag tcttgcatat aaccagtgtg aaattgagta tagaaatcat aatttaattt
     3361 aacggctaaa aaatacataa gcaatagaat gattaaaaat aaaagatatc taagagcttt
     3421 tttcatttta aattctccct tcagaaatac tttatttagt tttattttgt tggaatctga
     3481 ttttatcatt tagtttaaga aatccataat attagtgaag aatgaaataa gtattatata
     3541 tgaataaata tttttag
//
LOCUS       DBGJFF010000230         3490 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.235, whole genome shotgun sequence.
ACCESSION   DBGJFF010000230 DBGJFF010000000
VERSION     DBGJFF010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3490
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.235"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            95..2206
                     /locus_tag="ACOIAF_003766"
     CDS             95..2206
                     /locus_tag="ACOIAF_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding glycosyl-hydrolase Cwp19"
                     /protein_id="HGZ2670741.1"
                     /translation="MKKISILVLSLIMTLTMCSVSSFADSSNDKEMRAAWISTVYNLD
                     WPKTKNNEAKQKKEYTDLLDKLKSVGINTAVVQVRPKSDALYKSNINPWSEYLTGTQG
                     KDPGYDPLPFLIEEAHKRGMEFHAWFNPYRITMADESIDKLPANHPAKKNPSWVVKHG
                     NKYYYDPGLPEVRKYIVDSIAEVVQNYDIDGVHFDDYFYPGVSFNDTATYQKYGKGQN
                     KDDWRRENVNTLLRDVKASIKSIKPNVVFGVSPAGIWRNKSSDPTGSDTSGNESYVGT
                     YADTRAWIKQGLIDYVVPQLYWPIGLKAADYSKLVAWWANEVKGTNVDLYIGQGIYKQ
                     GQSSYGGQNIAKEIVQQVTLNRKYSEIKGSMYFSAKDIANSTSIQKDLKSLYSSSEEP
                     VTPPSNVKVEKLRGDERYDTAVAISKKGWATNSDTVVLVNGYSIVDGITSTPLATSND
                     APILLVNKDNIPTSTKNELKRLNPSKVILIGGNNSIGDKVESEIKDTLSNVSINRVGG
                     SDRYSTSLMIAKELVKTNPVEKLYITSGTGEADSLSIASKAGEEKQPIVLVSKDNVSD
                     EVYNWISDLKVKDAYFIGGNLSISDSVINKLDKVITNDVSKNRIAGENREETNGKVIQ
                     KFYPNAEYSSMFVSKSNQLVDALTSGPLAAKLKSPVVMLGNSVTSAQKTALEPKKTTL
                     VYEAGDGINQNTLNTFLNLVK"
     gene            complement(2404..3369)
                     /locus_tag="ACOIAF_003767"
     CDS             complement(2404..3369)
                     /locus_tag="ACOIAF_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HGZ2670742.1"
                     /translation="MSKLKKFVILLAFLVVIFPISVYGYFYYKLSAIHDSSISSDLLN
                     NNDHKNEDGIINILLMGTDARPNEDSSRSDAMMILTIDNKHNDIKLTSLARDSYVDIP
                     GHGKQKLTHAYAYGQADLLIQTIEENFNIDIQNYACVNFESFMYIIDAIGGVEVTVEK
                     GEIRELNKFIPETYKWNKSDDKGSIQYIRNSGKQTLNGYQALSFARIRHNDTAFARDG
                     RQRQIIQAIIKKTETLPVTKYPGLLDAVLPYVKTNMKPNAILSLGAQVLKMGDLNIKQ
                     FEFPIDDEIHSTGGIYGKAGWVLRFDPDTLDILHDFIFNDIEFKQ"
BASE COUNT         1356 a          464 c          582 g         1088 t
ORIGIN      
        1 ttattcaata tttgacagat aatatgaatt ttagatatac ttaataagaa gtatattgta
       61 gaaaaaataa atttaaaaaa ttaaggggga tgaaatgaaa aaaataagta tattagtatt
      121 atcattgata atgacactta ctatgtgtag tgtaagttca tttgctgatt catcaaatga
      181 taaggaaatg agagctgctt ggatatctac agtttataat ttagactggc ctaagactaa
      241 gaataatgaa gccaagcaaa aaaaggaata cacagattta ttggacaaat taaaaagtgt
      301 aggaataaat acagcagtgg ttcaagtaag accaaaatca gatgctttat ataaatcaaa
      361 tataaaccca tggtctgaat atcttaccgg tacacaaggt aaagaccctg gatatgaccc
      421 attaccattt ttaatagaag aagcacataa aagaggaatg gaatttcatg catggtttaa
      481 tccatataga ataactatgg cagatgagag catagataaa ttgccagcaa accatccagc
      541 aaagaaaaat ccaagctggg tagttaaaca tggaaataag tattactacg acccaggttt
      601 accagaagtt agaaagtaca tagtggatag tatagcagaa gtagttcaaa attacgatat
      661 agatggagtt cattttgatg attacttcta tccaggagta agtttcaatg atactgcaac
      721 ataccaaaaa tatggaaaag gacaaaataa ggatgattgg agaagagaaa atgtaaatac
      781 tctacttaga gatgtaaaag cgtctataaa gagtataaag cctaatgttg tatttggtgt
      841 aagcccagca ggtatatgga gaaataagag tagtgaccca acaggttcag atacatcagg
      901 taatgaaagt tatgtaggta catatgcaga tacaagagca tggataaaac aaggattgat
      961 tgactatgta gttccacaac tttactggcc aattggtctt aaagcagcag attattctaa
     1021 attagtggca tggtgggcta atgaagtaaa aggaacaaat gtagacttat acataggaca
     1081 aggtatatat aagcaaggtc aatcgagtta tggtggacaa aatatagcaa aagaaatagt
     1141 acaacaagta acattaaata gaaagtatag tgaaataaaa ggtagtatgt atttctcagc
     1201 taaagatata gcaaatagta cttctataca aaaggattta aagagtttat attctagtag
     1261 tgaagaacca gttactccac catctaatgt taaagtggag aaattaagag gcgacgaaag
     1321 atatgacaca gcagtagcaa taagtaaaaa aggttgggct acaaattcag atacagtagt
     1381 attagtaaat gggtactcta tagttgatgg tataacatca acaccacttg ctacatcaaa
     1441 tgatgctcct atactattgg taaataagga taatatacct acatccacta agaatgagtt
     1501 aaaaagactt aatccaagta aagttatttt aattggagga aataactcta ttggagataa
     1561 agttgagagt gagataaaag atacattgtc aaatgtatca ataaatagag ttggtggttc
     1621 agatagatat agtacatctt taatgatagc aaaagaatta gtaaaaacta atccagtaga
     1681 aaaactatat ataacaagtg gtacaggtga agcagattct ctttctatag cttcaaaagc
     1741 aggagaagaa aagcaaccaa tagtattagt atcaaaagat aatgtaagtg atgaagttta
     1801 caattggata tcagatttga aagtaaaaga tgcttatttt ataggtggta atctaagtat
     1861 atctgattca gtaataaata agctggataa ggtcattaca aatgatgtat ctaaaaatag
     1921 aatagctgga gaaaatagag aagaaacaaa tggaaaagta atacaaaaat tctatccaaa
     1981 tgctgaatat tcatcaatgt ttgtaagtaa aagtaatcaa ttggtagatg cattaacatc
     2041 aggtccttta gcagctaaat taaaatcacc agtagttatg cttggaaata gtgtaacatc
     2101 tgctcagaaa actgctttag agcctaagaa aactactcta gtatacgaag ctggagatgg
     2161 tataaatcaa aacacattaa acactttctt aaacttagtt aagtaataaa aaagttaatt
     2221 taaaataaag agtattttat aaatgtagat taaaatgaaa attatcatga taaattagaa
     2281 aataaaaaag aactatatgc attttgtaat caagtgtaca cagcaacttg aaaataatta
     2341 tgcgatatag ttctttttta gtaattaatc ttatactttt taataaactt tatgttgaat
     2401 tttttattgt ttaaactcta tgtcattaaa tataaaatcg tgtaatatgt ctaaagtatc
     2461 tgggtcaaat ctaagaaccc atccagcttt tccatatata ccacctgtac tatgaatttc
     2521 atcatcaata ggaaattcaa attgcttaat atttaagtcg cccattttta aaacttgagc
     2581 tcctaaagaa agtattgcat taggcttcat gttagtttta acatatggaa gaactgcatc
     2641 taataaacct ggatatttag taacaggtaa agtttccgtt ttcttgatta tagcttgtat
     2701 aatctgtctc tgtctgccat ctctagcaaa ggctgtatcg ttgtgtctga tacgtgcaaa
     2761 agatagtgcc tgatatccat ttagagtctg ctttccagag tttctaatgt attgtatgct
     2821 acctttatca tcacttttat tccatttata agtttcagga ataaatttat ttagttctcg
     2881 tatttctcct ttttcaacag taacctcaac gccaccaata gcatcaatta tatacataaa
     2941 tgattcgaaa ttaacacaag cataattttg aatatcaata ttaaaattct cttcaattgt
     3001 ttgtattaat aaatcagctt gtccataagc ataagcatga gtaagttttt gtttaccatg
     3061 accagggatg tctacatagc tatcacgagc taatgatgta agtttgatat cattgtgttt
     3121 gttatcaata gtaagtatca tcatagcatc tgaccttgaa gaatcttcat ttggtctagc
     3181 atctgttccc ataagtagta tgttaattat cccatcttca tttttatggt cattgttatt
     3241 caataaatca ctacttatgc tcgagtcatg aatagcagat aatttataat aaaaatatcc
     3301 atagacactt atagggaata ttactaccaa gaaagccagt agtataacaa atttctttaa
     3361 ttttgacaaa agtacccctt ctttcttctt tttcctgtat attttttaat agcatacact
     3421 taattatatt aaagtattga gatatattca atacttgttt cagttacaaa aaaaagacaa
     3481 tgatataatt
//
LOCUS       DBGJFF010000231         3343 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.236, whole genome shotgun sequence.
ACCESSION   DBGJFF010000231 DBGJFF010000000
VERSION     DBGJFF010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.236"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            <1..577
                     /locus_tag="ACOIAF_003768"
     CDS             <1..577
                     /locus_tag="ACOIAF_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exosporium glycoprotein BclB-related protein"
                     /protein_id="HGZ2670743.1"
                     /translation="GPTGPTGPTGATGATGATGASAIIPFASGIPLSLTTIAGGLVGT
                     PGFVGFGSSAPGLSIVGGVIDLTNAAGTLTNFAFSMPRDGTITSISAYFSTTAALSLV
                     GSTITITATLYQSTAPNNSFTAVPGATVTLAPPLTGILSVGSISSGIVTGLNIAATAQ
                     TRFLLVFTATASGLSLVNTVAGYASAGIAIN"
     gene            746..1507
                     /locus_tag="ACOIAF_003769"
     CDS             746..1507
                     /locus_tag="ACOIAF_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HGZ2670744.1"
                     /translation="MKNKIEISMNNFVKNYHKSNNITTKWQELLFAYASAEDDMFQKL
                     KSVVGTSHAMPKDFLENGKTIITYFIPFDESVNNSNIGGFECSEIWARAYIETNMLIS
                     NLNDFLCEELEKIGANSYAIPGSYNFDSEKLVSDWSQRHVAFIAGLGTFGLNNMLITE
                     KGCCGRIGSIITSLELEPTKRPENEYCLYKHNNSCKKCVNKCVKEALKEDGFNRNKCY
                     EMCMINEKKYTYIGGCDICGKCIVGVPCSVINPSR"
     gene            1964..3337
                     /locus_tag="ACOIAF_003770"
     CDS             1964..3337
                     /locus_tag="ACOIAF_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003500159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HGZ2670745.1"
                     /translation="MADDKTTKTPEQPVTDSGPGKETPPAPPKEPEKVSVSSEPGKKT
                     EPEVKNPQVSVYNFAEIMKEKKAEERAATPGVEKPDPAKVEKPEKQPETPKKAEEKPK
                     EPEQPKRRGRPPKADKDKAAAPKSKAPAQKPEKTVKKEPEKKTAPTVQTAPTPKEPEK
                     PQDAPRRGKEQIVYIKLNELHAFKNHPFEVRDDEEMRAMVSSVKDKGVTQPAIVRPRE
                     DGGYEIVSGHRRQKASELAGYADMPCIVRNLTDDEAITQMVEDNLNQREEILPSERAK
                     ALKMQLEAIKHQGSRTSGQIDPKDAGKRSNEIVAERNKMAVKQVQRYIRLNELVPDLM
                     KLMDEKKLGFTTAVELSYIGKKNQNYIAVAIDSQQSSPSQAQAKRMRELDEKKLLNGD
                     VIDGIMMEDKKEVDKVILTGAELSKYFGKETTPREMKDQIIKLLDDWKGQQKEHEKPE
                     KKTEQEK"
BASE COUNT         1161 a          623 c          772 g          787 t
ORIGIN      
        1 aggcccaaca ggcccaacag gcccaacagg agcaacagga gcaacaggag caacaggtgc
       61 tagtgcaata ataccttttg catcaggtat accactatca cttacaacta tagctggagg
      121 attagtaggt acacctggat ttgttggatt tggtagttcg gctccaggat taagtatagt
      181 tggtggagta atagacctta caaacgcagc aggaacattg actaactttg cattttcaat
      241 gccaagagat ggaacaataa catctatttc agcatacttc agtacaacag cagcactttc
      301 acttgttggt tcaacaatta caattacagc aacactttac caatctactg caccaaataa
      361 ctcatttaca gctgtaccag gagcgacagt tacactagct ccaccactta caggtatatt
      421 atcagttggt tcaatttcta gtggaattgt aacaggatta aatatagcag caacagcaca
      481 aactcgattc ttactagtat ttactgcaac agcttcaggt ctttcattag ttaatactgt
      541 agcaggatat gcaagtgcag gaattgcaat aaattagatt agataagaaa aggcttctaa
      601 tatcgtattg atattaggag cttttttaag aatgattgat gaacatagtt ttaatattaa
      661 ttagtgcgca tattataaaa tactattata taatataaaa taataacatt aaattggaaa
      721 tatagaatta tctaggggtg atactatgaa aaataagatt gaaatatcaa tgaacaactt
      781 tgtaaaaaat tatcataaat caaataatat aactacaaag tggcaagaac ttttgtttgc
      841 ttatgcatct gctgaagatg atatgtttca gaagctaaaa agtgttgtag gaacatctca
      901 tgctatgcca aaagattttc tggaaaatgg aaaaactata attacatatt ttataccttt
      961 tgatgaaagt gtaaataata gtaatattgg agggtttgaa tgttctgaaa tatgggcaag
     1021 agcgtacata gaaactaata tgttgatttc aaatttaaat gatttcttat gtgaagaact
     1081 agagaagata ggggctaatt catatgctat tcctgggtcg tataattttg acagtgaaaa
     1141 attggtaagt gactggtcac aaagacatgt agcattcata gcaggtcttg gaacttttgg
     1201 tttaaataat atgcttatta cagaaaaagg gtgttgtgga agaataggaa gcataatcac
     1261 aagtttagaa ttagagccaa ctaaaagacc agaaaatgaa tactgcttgt ataaacataa
     1321 taatagttgc aaaaaatgtg tcaataaatg cgtgaaagaa gctttaaaag aagatggatt
     1381 taataggaat aagtgttatg aaatgtgtat gataaatgaa aaaaaataca cttatatagg
     1441 tggatgtgat atttgtggaa agtgtatagt tggagttcct tgttcagtaa taaacccatc
     1501 aagataatgg atttaagttg actaataatt ttaaagatag tttataatga aaaaatttta
     1561 gttatcaaaa taaatataat ataaaaacta aataaatgtg tttaaaaaat ggataaataa
     1621 tttgtgttga ttgacaacca gttatactgt tgatagactt atatgtatcg ctgtgatata
     1681 taaggagggt tgagatttta tgtttgattt aatctttatt tttgcatagc aagggctatt
     1741 gcaactatga aatttaatat ataaatagcc ctttgcaacg gtgtacaaag ggaacataat
     1801 aaaaaactta ataatggact gccaatctta cacaaagtac cgcccaaacg agactacggg
     1861 cggtattttt gtatcatggc ggagaaagga ggaacttccc acgggggctt gactgaaaag
     1921 ttgagcccct tttttcatgc caaaaaacag gaggtaaatg cttatggcag atgataaaac
     1981 cacaaaaacg ccggaacagc cggtaacgga tagcgggccg ggcaaggaaa cgcctcccgc
     2041 tcccccaaaa gagccggaga aggtttccgt ttcctcggag ccgggaaaaa agacggaacc
     2101 agaggtaaag aacccacagg tttccgtcta taacttcgct gaaattatga aggaaaagaa
     2161 agccgaggaa cgggcggcaa ctcccggcgt ggaaaagcct gacccggcaa aagtggagaa
     2221 accggaaaag cagccggaga ctccgaaaaa agcagaggaa aaacccaaag agccggaaca
     2281 gccgaagcgc cggggccgtc ccccgaaagc agataaggac aaggccgcag ccccgaagtc
     2341 caaagccccc gcacagaaac cggaaaagac ggtcaaaaag gagccggaga aaaaaacggc
     2401 tccaacggtg cagaccgctc ccactccgaa ggaacctgaa aagccgcagg atgcaccacg
     2461 ccggggcaag gagcagatcg tctatatcaa gctgaatgaa ctccacgcct tcaagaacca
     2521 tccctttgag gttcgggatg atgaagaaat gcgggctatg gtgtccagcg tcaaggacaa
     2581 gggcgttacc cagcccgcta tcgtccgtcc ccgtgaggat ggcggctatg agatcgtgtc
     2641 cggccaccgc cgccagaaag ccagtgagct tgccggatat gcggatatgc cctgtatcgt
     2701 ccgaaacctg acagacgatg aagccatcac ccagatggtc gaggacaatc tcaaccagcg
     2761 tgaagaaatc ctacccagtg agcgggccaa agccttgaaa atgcagcttg aggctatcaa
     2821 gcaccagggc tcccgcactt cgggccaaat tgacccgaag gacgctggca aacgctccaa
     2881 cgagatcgtg gccgagcgca ataagatggc cgtcaaacag gtgcagcggt atatccggct
     2941 gaatgagctg gttcctgacc tgatgaagct gatggatgaa aaaaagctgg gctttactac
     3001 ggcggtggag ctttcctata tcggcaagaa gaaccagaac tatatcgccg tcgccattga
     3061 cagccagcag tcctcgcctt cacaggcgca ggcaaagcgt atgcgtgagt tggacgaaaa
     3121 gaagctactc aacggggatg tgatcgacgg cattatgatg gaggacaaaa aggaggtaga
     3181 caaagtgatt ttgacaggtg cggaactgag caagtatttc ggcaaggaaa ctacgccgag
     3241 ggaaatgaag gaccagatca tcaagctgct ggacgactgg aagggtcagc agaaggaaca
     3301 cgaaaagccg gagaagaaaa ccgaacagga aaagtaagcc caa
//
LOCUS       DBGJFF010000232         3324 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.237, whole genome shotgun sequence.
ACCESSION   DBGJFF010000232 DBGJFF010000000
VERSION     DBGJFF010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3324
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.237"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            287..1555
                     /gene="eam"
                     /locus_tag="ACOIAF_003771"
     CDS             287..1555
                     /gene="eam"
                     /locus_tag="ACOIAF_003771"
                     /EC_number="5.4.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016869 - intramolecular aminotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006536 - glutamate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 2,3-aminomutase"
                     /protein_id="HGZ2670746.1"
                     /translation="MNEQTRISLERAAELKSKIDDYIQARKTINRGLEKEEEINKRKQ
                     KILSILNGTEEDWNNYKWQLSNRITDVDTLSKIITLTKKEKEYIKEVGTQFRWAISPY
                     YLSLIDPEDICDPIKLLSIPTHIELEDEQEDLDPMGEEYTNPAGCITRRYPDRLIINV
                     TNECAMYCRHCQRRRNIGQQDSHKSKAIIQESIDYIRENEEIRDVLVTGGDALTLKDD
                     YLEWILSQLKEIPHVDYVRLGTRTLVTMPQRITDEFCNMLKKYHPVYINTHFNHPMEI
                     TKESKEACEKLANAGVPLGNQAVLLNGINNDKFVMRCLNQELLKIRVKPYYIFQSKHV
                     KGTKHFNTSVDDGLEIMEYLRGYTSGMAIPTYIVNAPKGGGKTPLLPQYLVSKGTDYV
                     MLRTWEGKVIKMEDEPAVDIKKLIKEQAQD"
     gene            complement(1648..2004)
                     /locus_tag="ACOIAF_003772"
     CDS             complement(1648..2004)
                     /locus_tag="ACOIAF_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670747.1"
                     /translation="MKKALISLLVLSCLLLTAPVNESSYADSQKNVPVSQNTKLTQEQ
                     AKELLVKYNNEVDYIYQGNASDFEALKAKNLNGYVFLPDADGDIGYFVNENTSEIYYF
                     HPSGYLELVNQQQNNK"
     gene            complement(2155..3249)
                     /locus_tag="ACOIAF_003773"
     CDS             complement(2155..3249)
                     /locus_tag="ACOIAF_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HGZ2670748.1"
                     /translation="MQDKKIYLNIVLTVVVSYILIKVIDNYKYFFGVISLLMSLLTPF
                     IIAFVLAYIFNPLVKFLESKLNFKRIYSLLLTYGVLIVILISFILFTVPSIVNSLADL
                     VAQIPTYIDKTEQFLFDLGKSLQSVDPNTLKEYGDKIMSVMPKFSNLLIGSLGGIFST
                     TFSVGKFIVQFLLGFIICFYILLEKEKFLLFTKKVVYISLGKKYGDFIIELCQSLNLN
                     IGKYFTGKILDSFIVGVLSGIGLYFLKSEYALLFGTLMGVMNMVPYFGPVIGMAPVVI
                     INLFSNPTIALTSLIYLIIIQQVEVTFIEPKIVGGQLGLSPFFTILAVTVGGGFFGIP
                     GMILSAPVMGVIKIYFCEFVNRRHDKIQME"
BASE COUNT         1357 a          435 c          475 g         1057 t
ORIGIN      
        1 taataatctt gaaaatataa tcgttttatg gtaaaataat tcttaaaaat agaaaaaatt
       61 aatcatttgt taaaaaaaat gcataattta ttgtaattta ttgacgaagg ttgaaaaata
      121 ttcataaatg tgcgaaataa cagaaaaaat gcaaaaatta attcaatttc atttgttata
      181 aaatctatat ggcaaaatta aatctgccta tcagaatgat ataaacacat cagaaaggag
      241 gacgctatca aataatacaa gtagttaact ttgatagcaa aaaattatga atgaacaaac
      301 tagaatatcc ttagagagag ctgctgaatt aaaatcaaaa attgatgatt atattcaggc
      361 tagaaaaacg attaacagag gtcttgaaaa agaagaagag ataaataaac gaaaacagaa
      421 aatattaagt atcttaaatg gaactgaaga ggattggaat aactacaaat ggcaattatc
      481 aaatagaata acagatgtag atactttatc aaaaattata actctaacta aaaaagaaaa
      541 agaatatata aaagaggttg gtactcaatt tagatgggca atatctccat attatttgag
      601 tcttatagac ccagaagata tatgtgaccc aataaaatta ctgtctatac caacacatat
      661 agagttggaa gatgagcaag aagatttgga cccaatggga gaagagtata caaacccagc
      721 aggatgtata actagaagat acccggatag attaataata aatgtaacaa atgagtgtgc
      781 tatgtattgt agacactgtc agagaagaag aaatattgga caacaagatt ctcataagtc
      841 aaaagctatt atccaagaat ctatagacta tatcagagaa aatgaagaaa taagagatgt
      901 actagtaact ggtggagatg ctcttacatt aaaagatgat tatttagagt ggattcttag
      961 ccaacttaaa gagataccac atgttgatta tgttagatta ggtactagga ctcttgttac
     1021 aatgccacaa agaattacag atgaattttg caatatgcta aaaaaatatc accctgtata
     1081 tataaatact cattttaatc atccaatgga aataactaag gaatctaaag aagcttgtga
     1141 aaagttagca aatgcaggcg ttccattagg aaatcaggca gtattattaa atggaataaa
     1201 taatgataaa tttgtaatga gatgtttaaa tcaagaatta ctgaaaataa gagtaaaacc
     1261 ttattatata ttccaaagta aacatgtaaa gggaacaaaa catttcaata catcagtaga
     1321 tgatggtctt gaaatcatgg agtatttaag aggatataca tcaggaatgg ctataccaac
     1381 atatatagta aatgctccaa aaggaggagg aaagactcct ttgcttcctc aataccttgt
     1441 atcaaaagga acagattacg ttatgcttag aacatgggaa ggaaaagtta taaaaatgga
     1501 agatgaacct gctgtagata taaagaaact tataaaagaa caagcacagg attaaaaaaa
     1561 tatttaaagt ttataaaaag aaacaacttt aaaataaaaa gaaaatggat gtcaaattta
     1621 tatcaatttg catccatttt ttattagtta tttattattt tgttgttgat taactaattc
     1681 aagatatcca cttgggtgga agtaatatat ctcagaagta ttttcattta caaagtaacc
     1741 aatatcacca tctgcatctg gtaaaaatac ataaccatta agatttttag cttttaatgc
     1801 ttcaaaatca ctagcatttc cttgatatat gtaatcaact tcattgttgt attttactaa
     1861 aagttcttta gcttgttctt gagtcaattt agtattttgg cttacaggaa cattcttttg
     1921 agaatctgca taagaagatt catttactgg tgcagttaat aacaaacaag ataaaactaa
     1981 taaagatata agtgcttttt tcatactaaa accctctttc atgtttaatt atataagatt
     2041 cattacttaa taatattgta tgtttcctat aattaaatat taccattaaa gagaataata
     2101 ttacagttac acagctgtaa cttttgatta caaaatcgta attaaattgt atttttattc
     2161 catttgtatt ttatcatgtc tacgatttac aaactcacag aagtatatct ttataacgcc
     2221 catcacagga gcagatagaa tcataccagg tataccaaag aatcctccac caacagtaac
     2281 tgctaggatt gtaaagaatg ggctaagacc tagttgtcca cctacgattt ttggctctat
     2341 aaatgtaact tcaacttgtt gaattattat taagtaaatt aaagaagtta aagcaattgt
     2401 tggattggag aataaattaa ttattacaac tggtgccata cctattactg gaccaaaata
     2461 tggaaccata ttcatgacac ccataagtgt accaaataat agtgcatatt cagattttaa
     2521 aaagtaaaga ccaattccag aaagtactcc aactataaaa gaatctaaaa tcttacctgt
     2581 aaagtattta ccaatattta aatttaagct ttgacatagt tctattataa agtctccata
     2641 ttttttacct aaggaaatat aaactacttt ttttgtaaac agtaagaatt tttctttttc
     2701 taataatata tagaaacaaa ttataaaacc taaaagaaat tgtactataa attttccaac
     2761 tgaaaatgta gtgctaaata tcccaccaag tgaaccaata agcaagttac taaatttagg
     2821 cattacactc ataattttgt caccatactc ttttaatgta ttagggtcaa ctgattgtaa
     2881 actcttgcct aagtcgaata aaaactgctc tgttttatct atgtatgtag gaatttgagc
     2941 aactaaatca gctaaactat ttactatgga tggaactgta aataatataa aggaaattag
     3001 tataactatg agtacaccat aagtaagaag taacgaatat attctcttaa aatttaattt
     3061 gctttctaag aactttacta gtgggttgaa tatatatgct aatacaaatg ctattataaa
     3121 tggtgttagt agtgacatta ataaacttat cactccaaaa aaatatttgt aattatcaat
     3181 aactttaatc aagatatatg aaacaactac agtgagtaca atatttagat aaattttttt
     3241 atcttgcaaa ataacacaac ctttcttata tttattaaat ttagatatac ttaaatctaa
     3301 gttaaagtta atatttatag ctta
//
LOCUS       DBGJFF010000233         3324 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.238, whole genome shotgun sequence.
ACCESSION   DBGJFF010000233 DBGJFF010000000
VERSION     DBGJFF010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3324
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.238"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(<1..1047)
                     /locus_tag="ACOIAF_003774"
     CDS             complement(<1..1047)
                     /locus_tag="ACOIAF_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HGZ2670749.1"
                     /translation="MTLFRIAMQNVKNSFFNYLMYFISIVFSVFVFFSFKSIEYNEAL
                     SSLGEKTRMSINTSSIVIVVFVFLFIYYSNSFFFNRRRREVGTYSLLGMRKNQIGKIF
                     LYETFLMGIVAILIGIFLGLLFSKLMTMMLVKLMNEMIVVNMNLSIKALVQTLAVFLI
                     IFIVIGIRNTIVIRNKRIIELFNKYPEKYKFKKFIKLKCVLGVLLITISYLMSISYFI
                     AENIMLSVFILVTIIPGTFLFFSSTMYMIIDAVKKRKSFYYKGQNLIAFSELGFKLKS
                     NSSVLAVIAILIATSVTMLGFTISLYYDIDRNISENYKYSYTINLGNSYVNNEIDKLL
                     YKYKKDNKIIFDKTI"
     gene            complement(1034..1801)
                     /locus_tag="ACOIAF_003775"
     CDS             complement(1034..1801)
                     /locus_tag="ACOIAF_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2670750.1"
                     /translation="MEEILYVENIKKEYGRKGSKHEALRGITFKVYKGEFVGIMGSSG
                     AGKSTLLNIISTIDLPSSGDIYINGKNTIKMKQNELADFRRDNLGFVFQDSNLLDTLT
                     IKENIMLPLSLKNERVSVIENRIKEISKELNIESILDKYPGEVSGGQKQRGAVCRAIA
                     TKPSLVLADEPTGALDSKSARDLLNCLLKLNKDSNKTILMVTHDAISASFCNRILFIK
                     DGIIFTEIVKGESNREFYNKIVNTVSLIGGVNKNDFI"
     gene            2089..2763
                     /locus_tag="ACOIAF_003776"
     CDS             2089..2763
                     /locus_tag="ACOIAF_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HGZ2670751.1"
                     /translation="MFKIMVVEDDVSLKNIIAKCLTKWGHDVHQIENLENIIEEFKNY
                     NPELVLLDINLPFYDGFHWCNEIRKISKVPIIFISSRNSNMDVIMGVNLGADDYIQKP
                     FSVDVLVAKVNALLRRTYNFVDNNSNQIIHNGVTLDLSTATINYEDNTIELTKNEIKI
                     LHELMKYKGQIVSRNKLMKKLWDNDWFVDDNTLTVNVNRIRSKLNEIGLEDFIETKRG
                     LGYIIS"
     gene            2787..>3324
                     /locus_tag="ACOIAF_003777"
     CDS             2787..>3324
                     /locus_tag="ACOIAF_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HGZ2670752.1"
                     /translation="MSFKDFLKDKLGFLVYNFTFLIFTVSVIIFSPVDVFLTDTILYI
                     LVVNVVFLFLYLFISYIKKNKFLDKIKNDTFKINMNDIELARCKNEEKIYLNIIKNHQ
                     YQCNNIINNNIEKFEENKEILNMWVHDVKIPIAIIKLILEQNENLIDEKISDDIDSEI
                     FRIENSVEKILYLSRLEDF"
BASE COUNT         1261 a          437 c          364 g         1262 t
ORIGIN      
        1 tattgtttta tcaaagatta tcttattatc ttttttatat ttatataata atttgtcaat
       61 ttcgttattt acataagaat ttcctaaatt tatggtataa ctgtatttat aattttcact
      121 tatgttccta tctatatcgt aataaagtga tatggtaaac cctagcatag ttacacttgt
      181 agctattaat attgctatta cagccaatac actactattg ctctttaatt taaatccaag
      241 ttcagaaaat gcaattaaat tctgtccctt ataataaaaa ctttttctct tcttaacagc
      301 atcaataatc atatacatag ttgatgaaaa aaataaaaaa gttccaggta ttattgtaac
      361 taatataaaa actgaaagca taatattttc agcaataaaa taactaattg acattaaata
      421 agaaatagtt attaataaaa caccaagtac acattttaac tttataaatt ttttaaactt
      481 atacttttct ggatatttat taaataactc tataattctc ttatttctga taactatagt
      541 atttcttatt ccaataacta taaatattat taaaaataca gctaatgtct gaactaaggc
      601 ctttatactt aagttcatat ttactactat catttcattc attaatttta caagcatcat
      661 agtcataagc tttgaaaata acaatcctaa aaatatacct attaatattg caactatacc
      721 catcaaaaaa gtttcatata aaaatatttt tcctatctga tttttcctca ttccaagtaa
      781 actataagtt cctacttctc gcctacgtct attgaaaaaa aatgagtttg aataatatat
      841 aaataaaaat acaaatacca ctattacaat tgaacttgta ttaatactca tcctagtttt
      901 ctctccaagt gaactcaacg cttcattata ctctatagat ttaaaactaa aaaatacaaa
      961 tacactaaat acaattgaaa taaagtacat taaataatta aaaaaactat tctttacatt
     1021 ttgcattgca attctaaata aagtcatttt tattaactcc tccaattaag gaaactgtat
     1081 ttactatttt attatagaac tctctgttac tttctccctt tacaatttct gtgaatatta
     1141 ttccatcctt tataaagagt attctattgc aaaaactcgc acttatagca tcatgagtta
     1201 ccatcaatat tgttttattg ctgtctttgt ttaattttaa tagacaattc aacaaatccc
     1261 tagcagattt agaatctaat gctcctgtag gctcatctgc aagtactaat gatggttttg
     1321 ttgctatagc tctacataca gctcctcttt gcttttgtcc accagaaact tcgccagggt
     1381 atttatctaa tatagattct atatttaatt cttttgaaat ctcttttatt ctattttcta
     1441 ttactgatac tctttcattt tttaaagaaa gaggcaacat tatattttct tttattgtaa
     1501 gtgtatccaa aagattagaa tcttgaaata caaagcctag attatcacgt ctaaaatctg
     1561 ccaattcatt ttgcttcatt ttaatagtat tctttccatt tatataaata tctcctgatg
     1621 aaggtaaatc tattgtagaa ataatattta aaagtgttga tttaccagca ccggaagacc
     1681 ccataatacc aacaaattct cctttataaa ccttgaaagt tattcctctt agagcttcat
     1741 gttttgaacc ttttcttcca tactcctttt ttatattttc aacatataat atttcttcca
     1801 tagttaataa tcacctttca tcacaattag ttatttatca attctcacaa atatattata
     1861 tatattaaaa tggcttttaa acatttaatt atattaattt aaggttacat tttcgtaagt
     1921 attaattata tttcttcaaa tttattgcaa ttatgtttaa aatataattt atcaagtaaa
     1981 atatagttat attaatataa ttattttagg taataaggta gaaatatttt aatttatcaa
     2041 gtagaatata gttatgttaa tataactatt ttgaattagg aggtaagaat gtttaaaata
     2101 atggtagttg aagatgacgt ctcattgaaa aatatcatag caaagtgtct tactaaatgg
     2161 ggacatgatg ttcatcaaat agaaaatctt gaaaatataa tagaagagtt caaaaactat
     2221 aacccagaat tagttttatt ggacataaac cttccatttt atgatggttt tcattggtgt
     2281 aatgaaataa gaaaaatttc aaaagttcct ataattttta tttcttcaag aaattctaat
     2341 atggatgtaa taatgggtgt aaatttaggt gcagatgatt atatacaaaa acctttttct
     2401 gtagatgtct tagtagctaa agttaatgcc cttttaagaa gaacttataa ttttgttgat
     2461 aataactcaa atcagattat tcacaatggt gttactttag atttatcaac tgcaacaata
     2521 aactatgaag ataatactat tgagctaaca aaaaatgaaa tcaaaattct ccatgagctt
     2581 atgaaatata aggggcagat agtaagtagg aataaactca tgaaaaaatt atgggataat
     2641 gattggtttg tagatgataa tacattaact gtcaatgtaa atagaatacg ctcaaaatta
     2701 aatgaaattg gtttagaaga ttttatagaa acaaaaagag gattgggtta tataatcagt
     2761 taaaacaatt ctaagtaggt gaatttatga gctttaaaga ttttctcaag gataaactag
     2821 gttttttagt atacaatttt actttcttaa tttttacagt atcagttatt atattttctc
     2881 ctgtagatgt ttttctgact gatactattt tatatatatt agttgtaaat gttgtgtttt
     2941 tatttttata tctatttatt agctacatca agaagaacaa atttttagat aagataaaaa
     3001 atgatacatt caaaattaat atgaatgata tagaattagc tagatgtaaa aatgaagaaa
     3061 aaatatactt gaacattatt aaaaatcatc aatatcaatg caataacata ataaataata
     3121 atatcgagaa atttgaagaa aacaaagaga ttttaaatat gtgggtacat gatgtaaaga
     3181 tacctatagc tataataaaa ctcattcttg aacaaaatga aaatttaatt gatgaaaaaa
     3241 tttcagatga catagatagt gaaatattta gaattgaaaa ctccgttgaa aaaattttat
     3301 atttatcaag actcgaagat tttc
//
LOCUS       DBGJFF010000234         3285 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.239, whole genome shotgun sequence.
ACCESSION   DBGJFF010000234 DBGJFF010000000
VERSION     DBGJFF010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.239"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            97..1533
                     /locus_tag="ACOIAF_003778"
     CDS             97..1533
                     /locus_tag="ACOIAF_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp28"
                     /protein_id="HGZ2670753.1"
                     /translation="MKFYKRILTLTLSISFVFANIQLVDAISSVEKIQGKNKYEIAGK
                     IADKNAYKTAILINTSNSIADGLSASGLAGALNAPILLTEKNTIPTETSARLKNVSKV
                     YIIGGTYSISTSVENSLKSKKMKVVRIKGNDRIKTSYNVAKEINSIKKVNTVMLTNAY
                     KGEADAISIASVAARDKAPIILTNGQSIPFSTSGLKSYVIGGTASMSNTLVNNTKSTR
                     LGGSTRFETNKAIINKFYKDAREFYIAGAYELTNALVGSSLSKHGPMVLVNDGSNKSI
                     LKNAKKITSIGYIDSNIVQQCLNITNGIGDINTGVVKNVKPTTKTIKDGMYKVGKDIS
                     AGEYLITSNSGSYASYYEVTSDSTGNADSILSNDIFSGTRYITLKNGQYIKIEDSTMT
                     LAKYAKAQKAKNGKFGNGMYKIGLEIPAGEYIIMSNSSDAYYEVRNDSLGNAEGIVTN
                     DTFSGRRYITVEEGQYLILNDCYLIENE"
     gene            1781..3217
                     /gene="miaB"
                     /locus_tag="ACOIAF_003779"
     CDS             1781..3217
                     /gene="miaB"
                     /locus_tag="ACOIAF_003779"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HGZ2670754.1"
                     /translation="MSKRKQVTIPIEKIQEQDKYIEQIHRQNEEYFNRTGKRKLVFTQ
                     TFGCQMNEHDSEKLCSMLEEMGYQMSMMVEESDLIIYNTCAVRENAELKVYGNLGQLK
                     HLKGKNPDMKIAVCGCMMQQPHVVEELRKKYKHVDLIFGTHNLYKFPQLLTESINSDK
                     MLVDVWDVDGEVIEGLRSNRKFELKAFVNIMYGCNNFCTYCIVPYTRGRERSRTPEDI
                     INEIKELVANGTKEITLLGQNVDSYGKTLENPVSFSELLRKVNDIEGIERVRFMTSHP
                     KDISDEVIYAIRDCDKVCEFLHLPIQCGSSSLLKKMNRHYTKEYYLEIIEKAKKEVPG
                     IAFSTDLMIGFPGETEEDLLDTLDVVEKVRYDSAFTFIYSKRQGTPAAKMENQIPEDI
                     KHDRFNRVLEAVNRISAEINDGYKDKIVEVLVEGRSKNNENKFAGRTRQNKLVNFEGG
                     NDDLIGKLVMVKITEPRTFSLNGILVNN"
BASE COUNT         1378 a          332 c          571 g         1004 t
ORIGIN      
        1 taatttgcaa ttttacataa gtttgttaag tacataacta atttaagaat atgtatttat
       61 atattaaaaa tttattatga gggggacaat tttattatga aattctataa aagaatatta
      121 acattgactt taagtatttc ttttgtattt gcaaatattc aattagtaga tgcaatatca
      181 tctgtagaaa aaatacaagg aaaaaataag tatgaaatag caggtaaaat agcagataaa
      241 aatgcatata aaactgctat attaattaat actagtaact ctattgcaga tggattaagt
      301 gcaagtggac tagcaggagc tttaaatgcc cctatactat taactgaaaa aaatactata
      361 ccaactgaga catctgcaag attaaaaaat gtatcaaaag tttatataat tggaggaaca
      421 tattctatta gtacatctgt cgaaaattca ttaaaatcta agaaaatgaa agttgtaaga
      481 attaagggaa atgacagaat aaaaacaagt tataatgttg caaaagaaat aaattcaata
      541 aaaaaagtca atactgttat gctaaccaat gcatataaag gagaagcaga tgctataagt
      601 attgcatctg tagcagcaag agataaagca ccaataattc ttactaatgg acaaagtata
      661 cctttttcaa ctagtggttt aaagtcttat gtaataggag gaacagcttc aatgagtaac
      721 actttagtaa acaatactaa atctactaga ctagggggga gtactcgttt tgaaactaat
      781 aaagcaataa taaataagtt ttataaggat gctagagagt tttatatagc tggagcttat
      841 gaattaacaa atgcacttgt aggatcttct ttatcaaaac atggtccaat ggttttagtt
      901 aatgatggta gcaataaaag cattttaaaa aatgctaaga agataacttc tataggatat
      961 atagattcaa atatagtaca acagtgttta aatataacaa atggaattgg agatattaat
     1021 actggggttg taaaaaatgt taaacctact acaaagacta taaaagatgg aatgtataag
     1081 gttggaaaag atatatcagc aggtgaatac ttaataactt caaactcagg aagttatgct
     1141 tcttattatg aagttacaag tgatagtaca ggtaatgctg atagtatact ttcaaatgat
     1201 atttttagtg ggactagata tatcacctta aagaatggtc aatatattaa aattgaggat
     1261 tcaacaatga cattagcaaa gtatgctaaa gcccagaagg ccaagaatgg taagtttgga
     1321 aatggtatgt ataaaattgg attagaaatt cctgctggag aatatattat aatgtcaaat
     1381 tcttctgatg catattatga agttagaaat gatagcttag gtaatgcaga gggaatagtt
     1441 actaatgata ctttttcagg aagaagatat ataacggttg aggaaggtca atatttaata
     1501 ttaaatgatt gttatttaat tgaaaatgaa tagatgtaat tgtatttaat agtaaatcat
     1561 atattttatt taaggaaaga tgttttaagt tttaatttaa aacatctttt tattattata
     1621 ataattatat gattttatat tttagaaata tagtatatat aaatcattag atttactatt
     1681 aaaacacaac tagaaaaata ataatatctt taaaagattt ttcttatttg atataattaa
     1741 ggttatgtgt aatataagaa attaactagg aggaatagac gtgagcaaaa gaaaacaagt
     1801 aacaatacct atagagaaaa tacaagaaca agataaatat atagaacaaa tacacagaca
     1861 aaatgaagaa tacttcaaca gaactggaaa gagaaaactc gtgtttactc aaaccttcgg
     1921 gtgtcaaatg aatgaacatg attcagaaaa attatgttca atgttagaag agatgggata
     1981 ccaaatgtca atgatggttg aagaaagtga tttaataata tataacacct gtgcagttag
     2041 agaaaatgca gagctaaaag tgtatggtaa cttaggacaa ttaaaacatc taaaaggaaa
     2101 aaatccagat atgaaaatag ctgtatgtgg atgtatgatg caacaaccac atgttgtaga
     2161 ggaattaaga aaaaaatata aacatgtaga ccttatattt ggaacacata atctatataa
     2221 atttccgcaa ttacttacag aatcaataaa ttctgacaaa atgttagtgg atgtatggga
     2281 tgtggatgga gaagtaatag aaggtcttag aagtaataga aaatttgaat taaaagcatt
     2341 tgtaaatata atgtatggat gcaataattt ctgcacatat tgtatagtcc catacacaag
     2401 aggaagagaa agaagtagaa ctcctgaaga tataattaac gagataaaag agcttgttgc
     2461 aaatggaacg aaagaaatca ctttacttgg acaaaatgta gattcttatg gtaagacact
     2521 tgaaaatcca gtttcattct cagaactttt aagaaaagtg aatgatatag aaggaattga
     2581 aagggttcga tttatgacat ctcatccaaa agatatatct gatgaagtaa tatacgcaat
     2641 tagagattgt gataaggtgt gcgagttttt acatctacca atacaatgtg gaagcagtag
     2701 ccttttgaaa aaaatgaata gacattatac aaaggaatat tatttagaaa taatagaaaa
     2761 agctaaaaag gaagttccag gtattgcttt ttctacagac ttaatgattg gtttcccagg
     2821 agaaacagaa gaagatttat tggacacttt agatgtggtt gaaaaagtta gatatgattc
     2881 agcttttaca tttatttatt ctaaaagaca gggaactcct gctgctaaaa tggaaaatca
     2941 aataccagaa gatataaagc atgatagatt taatagggtg ttagaagctg taaataggat
     3001 ttctgcagaa ataaatgatg gatataaaga taaaatagtt gaagttctag ttgaaggaag
     3061 aagtaaaaat aatgaaaata agtttgctgg tagaactaga caaaataaac tcgtaaattt
     3121 cgaaggtgga aatgatgatt taattggtaa attagttatg gtcaaaatta ctgaacctag
     3181 aactttttca ttaaatggga tattagtaaa taattaatat tgtacaaaat aataaaatta
     3241 tgtaaactaa tcaaaataga tgtactatag aacaaattat attaa
//
LOCUS       DBGJFF010000235         3259 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.240, whole genome shotgun sequence.
ACCESSION   DBGJFF010000235 DBGJFF010000000
VERSION     DBGJFF010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3259
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.240"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            100..2457
                     /locus_tag="ACOIAF_003780"
     CDS             100..2457
                     /locus_tag="ACOIAF_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HGZ2670755.1"
                     /translation="MDKVNLERYSCDINVGLNKNQLQSRIDDNLINAFDNGKTKTYKQ
                     IFKDNIFTLFNLINIVLVCLLVSVGSFKNTLFIFIAVINTIIGVIQEIRAKRLLDNLS
                     VIVSNKVSVIREKEKKEIDVQELVLDDIMILKTGNQICADSKVLNGKLEVNESLVTGE
                     SDIIIKNKGDFLYSGSFVVSGEAFIRVENIGKDNYANKIANDAKISKKHNSQLRNSLN
                     AILKIVGIIIIPLGIILFAKQHFGNGDSIATSVVGTVAAVNGMIPEGLTLLTSIALAV
                     GTIKLASHKTLVQELYCVETLARVDTLCLDKTGTITEGKMQVDDIVMLEEVDINEIMG
                     NICNSLKDDNATLNAIRDKYNVLDTYKAKNLIPFSSERKYSGVTFENKGTYLLGAFEF
                     IFKEKNKKLRIEVERYSKKGNRVIVLAHSDSYMEDNNIPEDIKPLAFILILDKIRDEA
                     KETLEFFYNEGVDIKIISGDNPITVSEVARKAGLKTASNFIDATTLKNDSDIYKAVDK
                     YSVFGRVSPQQKKQMILALKKQNHTVAMTGDGVNDVLALKEADCSIAMASGSDVTKNV
                     SNLVLLDSNFASMPKVVMEGRKVINNIQRASSLFLVKTIFSFFLSLLTLFFFSRQYPF
                     VPIQLSLIGAVTVGIPSFFLALEANKSRISGNFLLNILKNALPGALCVTVNVIIVYNI
                     VEYLGYSSSVFSTMCAILTGVCGLLILRQQCLPFNKERLFLIVSMTIAFVIGILFLGG
                     LFSFVALSRELILITMALAILMPIMIKVMLFVLDEILETIVPDLN"
     gene            complement(2543..2860)
                     /locus_tag="ACOIAF_003781"
     CDS             complement(2543..2860)
                     /locus_tag="ACOIAF_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HGZ2670756.1"
                     /translation="MSEFKNVTAVKKANVYFDGKVSSRVIILPNGERKTLGLMLPGEY
                     TFSTREEEIMEMLAGSMDVKLPGSNEFVTYKEGQKFNVPSDSSFDLKVNEVVDYCCSY
                     IAD"
BASE COUNT         1265 a          351 c          525 g         1118 t
ORIGIN      
        1 attatataaa ctatagaata taaaataaaa aaattgagaa ttttataata tatttgtaga
       61 tttgagtata aaaaagtgct aagttgaaag gagtaaaata tggataaggt taatttagaa
      121 aggtatagtt gtgatataaa tgttggttta aataaaaatc aacttcaaag tagaatagat
      181 gataatttaa ttaatgcatt tgataatgga aaaaccaaaa catataaaca aatttttaaa
      241 gacaatatat ttacactttt taatctcata aatattgttt tagtctgttt attagtttca
      301 gttggttctt ttaaaaatac cttatttata tttattgctg taattaatac tattatagga
      361 gtaatacaag agataagagc taaaagatta ttggacaatc tatctgttat agtttcaaat
      421 aaggtatctg tcataagaga aaaagagaaa aaagagattg atgtacaaga attagtatta
      481 gatgatatta tgattttaaa aacaggtaat caaatttgtg cagattcaaa agttttaaat
      541 ggaaaattag aggtaaatga gtctttggtt acaggtgaat ctgatattat tataaaaaat
      601 aaaggcgatt ttttatattc tggaagtttt gtagtttctg gagaagcttt tataagagtt
      661 gaaaatatag gaaaagataa ctatgcaaat aagatagcta atgatgcaaa gatttctaaa
      721 aaacataatt ctcaacttag aaattcatta aatgcaatat taaaaattgt aggtataata
      781 ataattcctc taggaattat attgtttgca aaacaacatt ttggaaatgg agattctata
      841 gctacatctg tagtaggtac agtggcagct gtaaatggta tgataccaga gggattaaca
      901 ttacttacga gcattgctct agctgtagga actataaaac ttgctagtca taaaacttta
      961 gtgcaagaac tgtactgtgt agagactctt gctagagttg atacactatg tcttgataag
     1021 acaggtacaa ttacagaagg taaaatgcaa gttgatgata tagtaatgtt ggaagaagtg
     1081 gatattaatg aaataatggg gaatatatgt aattctctca aagatgataa tgcaacacta
     1141 aatgcaataa gagataagta taatgtttta gatacgtata aagctaagaa tttaatacca
     1201 ttttcttcag aaagaaaata cagtggggtt acctttgaaa ataaaggtac atatttatta
     1261 ggtgcatttg agtttatatt taaggaaaaa aataaaaaac ttaggataga agttgaaaga
     1321 tattctaaaa aaggaaacag agtaatagtt ttagctcata gtgattctta tatggaagat
     1381 aataatattc ctgaagatat aaagccacta gcttttatat tgatattgga taaaattaga
     1441 gatgaagcta aggaaacttt agaatttttt tacaatgaag gtgtagatat aaaaattata
     1501 tctggtgaca atcctataac agtaagtgag gtagctagaa aagcaggtct taaaacagct
     1561 agtaatttta ttgatgcaac aacacttaaa aatgatagtg atatatacaa agcagttgat
     1621 aaatacagtg tttttggaag ggtaagccca caacagaaaa aacaaatgat tttagcatta
     1681 aaaaaacaaa atcatacagt agctatgact ggagatggtg taaatgatgt actagcactt
     1741 aaagaagcgg attgtagcat tgccatggca tctggaagtg atgttactaa gaatgtgtct
     1801 aatttagtct tattagattc aaactttgca tcaatgccaa aggttgttat ggagggaaga
     1861 aaagtaatta ataacattca aagagcatct tctttgtttt tagtaaagac aattttttca
     1921 ttcttcttgt cgttattaac attattcttc ttcagtagac agtatccatt tgttcctata
     1981 caattatcac tcattggggc tgtaacagtg ggtataccat cattcttctt agctcttgaa
     2041 gcaaacaaaa gtagaatttc aggaaatttt ttactcaata tattaaaaaa tgctttacca
     2101 ggagcactat gtgtgacagt aaatgtaatt attgtttaca atatagttga atatttaggt
     2161 tatagttcaa gtgtattttc cacaatgtgt gctatattaa ctggtgtatg tggtctttta
     2221 atattgagac aacaatgttt gccatttaat aaagaaagat tatttttaat agtaagtatg
     2281 actattgcat ttgttattgg gattctattt ttaggtggat tattctcatt tgtagcattg
     2341 agtagagaat taattctcat aactatggca ttagcaatat taatgccaat tatgataaaa
     2401 gttatgttat ttgtcttaga tgagatatta gagacaattg ttccagattt aaattaattt
     2461 taaaatgata taaataagag cagttttagt tgaactgctc tttttagatg tgtttaattt
     2521 tatgtaagaa tatgtatttt tattaatcag ctatatatga gcaacagtag tcaacaactt
     2581 cattaacctt taagtcaaaa cttgaatcac taggaacatt aaatttttgc ccttctttat
     2641 aagttacaaa ctcattgctt ccaggcagtt ttacatccat acttccagca agcatctcca
     2701 ttatttcttc ttctctagta gaaaaagtgt attctcctgg caacattaat cctaaagttt
     2761 ttctttctcc attaggcagt attattactc tgctacttac cttaccatca aaataaacgt
     2821 ttgccttctt tactgcagta acattcttaa attcactcat tagataaaac ctccaaaatt
     2881 atttgtacat atatattata taaaaataat gtaaaaaatg ctatatattt acaaaaaaat
     2941 ttagtattta ttaaaaaaat taataagaat aaaaaagagg tgaaattgct taaattgata
     3001 aaaaatataa aataaattgc aaagtaagca agaaaagaat aagttgaaga gttatatatt
     3061 ataaatatta aaaattttag tattaataaa aaagatatta cattaatatg aaaatatagg
     3121 agattagaag tgtttgatta aaaaatagct acacacactt gtgactttag tcatgagtta
     3181 gtagctaaaa tagtcagcat atagggaaac ttatatgtag agataggata gaacctatcc
     3241 aatactactt gaattgcta
//
LOCUS       DBGJFF010000236         3238 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.241, whole genome shotgun sequence.
ACCESSION   DBGJFF010000236 DBGJFF010000000
VERSION     DBGJFF010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3238
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.241"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(<1..619)
                     /locus_tag="ACOIAF_003782"
     CDS             complement(<1..619)
                     /locus_tag="ACOIAF_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HGZ2670757.1"
                     /translation="MFNLIRKDLIIGISSDGIRNLKYILLFFVFYFFLNSISYYTVSI
                     VVSYLIFINTFECDYENDSRIFIRSMPVSIEDIVYSKYLLGVGLIISVTIIVSLLSKL
                     TSLVFFRNMVLNDVFFSVNIFLAILTILLPLIFKFGYGKMKVCGFIVSILIYFVYGSL
                     LKMISMIVYQVKHFNYSKVGGVYLSNYVTDIANTKYINLYSMTFLT"
     gene            complement(619..1479)
                     /locus_tag="ACOIAF_003783"
     CDS             complement(619..1479)
                     /locus_tag="ACOIAF_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2670758.1"
                     /translation="MFSIRNITKKLRKFKLNNINLELKEGDIVGIIGPNGSGKTTLIK
                     IIMGIIDADEGEIELCNETIENSPISFKNNIGFVYDSLQFYPHLKVKEFRKIVSLFYK
                     NFDRERFDEYLNKFDIEENMHIENLSKGQSEKLMLSSALSHNAKLLILDEPTAGIDPI
                     VRTEIMQYLQDFVKNGSSSVIISTHNTDNLIKIADYLVFINRGNQIFTVKKELIEQEY
                     KIIRANKAELEAIKESIVGVKEYKYYNEALVKVGDSLKVKSLLIEIDKHKVKNPTIEE
                     LMYYYVNEVR"
     gene            1614..2414
                     /locus_tag="ACOIAF_003784"
     CDS             1614..2414
                     /locus_tag="ACOIAF_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HGZ2670759.1"
                     /translation="MKRIITINTNQLNKSLETKELLTRKLINAGFEVYSDIYPDTELI
                     ISIGGDGSFLRTVRDFDFPEIPIMGINTGHLGFFPDILPDKIDSFIEAYTKKDYIIQE
                     MSLLNAEVYTTTSGSNMLAVNEVVIRGDKSRTIHLNLSLDNKHIQNFSGDGMIISTST
                     GSTAYNYSAGGSIVDINLELMQITPLHPINTNAYRCFTSSIICSNESVIKIAPEYRFE
                     DSVLIVVDGVEHRFRQIENIKVSISDAKIKLLRMSNYEFWHRVSEKFL"
     gene            2439..3008
                     /gene="yfcE"
                     /locus_tag="ACOIAF_003785"
     CDS             2439..3008
                     /gene="yfcE"
                     /locus_tag="ACOIAF_003785"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HGZ2670760.1"
                     /translation="MKIGIMSDTHGSLLYFEKALNVLSDCDVLLHGGDVLYHGPRNDI
                     PEGYNPKKFIETLNKLENIVIVKGNCDADVDQMVIEHPIQSPYVMSQFGEIRIILNHG
                     YIESEEEIIDKAKKMGGDILVLGHTHVKKLYMDDNLIVINPGSTSIPKDGSHSVAIID
                     IIKTDDEDELELDINLIDINTGNIININD"
BASE COUNT         1206 a          470 c          390 g         1172 t
ORIGIN      
        1 ttgttaaaaa tgtcatacta tataaattta tatacttagt attagctata tcagttacat
       61 aatttgaaag atatactcct ccaactttac tataattaaa atgctttact tgatatacaa
      121 tcatactaat catcttcaaa agacttccat atacaaagta aattaaaata cttactataa
      181 aaccacaaac tttcattttt ccatatccaa acttaaaaat taaaggcagt aaaatcgtca
      241 aaattgctaa aaatatattt acagaaaaaa atacatcatt taaaaccata tttctaaaga
      301 acactagtga tgtcaattta cttagtaagg acacaattat tgttacagaa attattaatc
      361 ctacacctaa taaatatttt gagtaaacta tatcttctat actaactggc atactcctta
      421 taaaaatcct tgaatcattt tcataatcac actcaaacgt atttataaat atcaaataac
      481 taacaacaat acttactgta taatatgaaa ttgaattaag gaaaaaataa aacacaaaaa
      541 atagcaatat gtactttaaa ttacggatac catctgaact aatccctatt attaaatctt
      601 ttcttattaa attgaacatc atctcacctc atttacataa taatacatta gttcttctat
      661 agtaggattt ttgaccttat gcttatcaat ttctatcaat aggcttttta ctttcaagct
      721 atctccaact ttaacaagtg cctcattata atatttatac tctttcacac caactatgct
      781 ttctttaatt gcttctaact ctgctttatt tgccctaatt atcttatatt cttgttctat
      841 taattctttc ttaacagtaa aaatctgatt tcctctattt ataaaaacaa gataatcagc
      901 aattttaatt aaattatcag tattatgagt agaaattatt acactactac ttccattttt
      961 cacaaaatct tgtaaatact gcattatttc agttcttaca attgggtcta tcccagcagt
     1021 tggttcatcc aaaatcaata acttagcatt atgactaagt gcgcttgata acataagctt
     1081 ttcagattga cctttagata aattttcaat atgcatattt tcttctatat caaacttatt
     1141 aaggtattca tcaaaccttt ctctatcaaa attcttataa aataaagaaa ctatttttct
     1201 aaattcctta acctttaaat gcggataaaa ttgtaaagaa tcatatacaa aaccaatatt
     1261 gtttttaaag cttattggag aattttcaat agtttcatta caaagttcaa tttcaccttc
     1321 atctgcatca ataatcccca ttataatttt tattaaagtc gtttttcccg aaccatttgg
     1381 tcctataatc ccaacaatat ccccttcctt taactctaag tttatattat ttaacttgaa
     1441 ttttcttaat ttttttgtga tattccttat gctaaacaca tcttcacctc ttatgcattt
     1501 ttttataccc acctatacat attttaacac attcattaca aaggtcattt ataaatttat
     1561 aaattaagaa ttataaaact atgttataat acaaagtatg aagaggtgaa tatatgaaaa
     1621 gaattataac tataaataca aaccaattaa ataagtccct tgagactaaa gaactcttaa
     1681 ctagaaaact tatcaatgct ggctttgaag tgtattcaga tatttatcca gacacagagc
     1741 ttattatatc aattggtggt gatggctctt ttttaagaac agttagagat ttcgattttc
     1801 ctgagattcc cataatggga ataaatacag gtcatctggg gttttttcct gatattttac
     1861 cagataagat tgatagcttt attgaagctt atacaaaaaa agattatatt attcaagaaa
     1921 tgtcacttct taatgcggaa gtatatacaa ctactagtgg ttctaatatg cttgctgtaa
     1981 atgaggttgt tataagagga gacaagtcta ggactataca cctaaattta agccttgata
     2041 ataaacatat acaaaatttt agtggagatg gtatgattat ttctacttcc acaggttcaa
     2101 cagcctataa ctattctgct ggaggtagta ttgttgatat aaatcttgaa cttatgcaga
     2161 ttacaccttt acatccgata aataccaatg catataggtg ctttacatca agtataatat
     2221 gttcaaatga atctgttata aagattgcac cagaatatag atttgaagat tctgtattga
     2281 ttgtcgttga tggagttgag cacagattta gacaaatcga aaatataaaa gttagtatat
     2341 ctgatgcaaa aataaaatta cttagaatgt caaactatga gttttggcat agagtatctg
     2401 aaaaattttt atagactgta cttaggagga tttttaatat gaaaatagga ataatgagtg
     2461 atactcatgg tagcttactt tactttgaaa aagcgttaaa tgtattatct gattgtgatg
     2521 ttttattaca tggtggagat gtcttatatc atggacctag aaatgatatt ccagaaggat
     2581 ataatcctaa aaaatttatt gagacattaa ataaattaga aaacattgtg attgtaaaag
     2641 ggaactgtga tgcagatgtt gaccagatgg ttatagaaca tcctattcag agtccttatg
     2701 tcatgagcca atttggtgaa attagaatta tattgaatca tggttatata gaatcagaag
     2761 aagaaattat tgacaaagca aagaaaatgg gtggtgacat attagtttta ggacatactc
     2821 atgtaaaaaa gctatacatg gatgataatt taatagtaat aaatccagga agtacttcta
     2881 tcccaaaaga tggaagccat tctgtcgcaa ttatagatat cataaaaact gatgatgagg
     2941 atgagcttga acttgatata aacttaattg acatcaatac tggaaatata ataaatatca
     3001 atgattagta aatttttaat aaaaataaaa taagctttaa tttattaaac tcataataaa
     3061 aacaagtaaa ccattttatt tctatctcaa ttagaagttt aagtacaata ccttaaactt
     3121 ctaattaaat agaatctggt ttacttgttt tattatatgc tatattgaaa tataacttag
     3181 tttacttgtt tctattatat attatattgg agtataattt agtttactta tttcatta
//
LOCUS       DBGJFF010000237         3209 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.242, whole genome shotgun sequence.
ACCESSION   DBGJFF010000237 DBGJFF010000000
VERSION     DBGJFF010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3209
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.242"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            360..1073
                     /locus_tag="ACOIAF_003786"
     CDS             360..1073
                     /locus_tag="ACOIAF_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGZ2670761.1"
                     /translation="MDTCFNKKILLVDDEKDIVDLIEEVLINDGFKNIIKAYNGLDAI
                     SLCKIACPDVVILDIMLPDIDGIEVCKKIREFSYCSILFLSSKNDDIDKILGLSSGGD
                     DYITKPFSPREIAFRVKAQLRRQQYQSIVPSDSEVIKIGDITIDIEGNRVYKDRNEIE
                     LTGREYHLLSYMAQNVNKIIGKERLYEQVWGVYSSICDNTIMVHIRHIREKIEDNPSN
                     PKILITVKGLGYKLVNRID"
     gene            complement(1345..2883)
                     /locus_tag="ACOIAF_003787"
     CDS             complement(1345..2883)
                     /locus_tag="ACOIAF_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HGZ2670762.1"
                     /translation="MSLLKVTNLSQCFMDKSLYEKANFDLFKGEHIGVVGQNGTGKST
                     LIKILLGEVVPDSGEIKWQPNINIGHLDQYAEINRDTTISLYLHTAFEELYKIEKEMN
                     LLYQKSAISENEQYLIKASDYQEQLIANNFYSIDNEINKIANGLGLDSIGMNRVVGEL
                     SGGQRAKVILAKLLLSNHDVLLLDEPTNFLDKEHVEWLSNYLNTFNGAFIVVSHDFDF
                     LEKISTGILDIEFGMIKKYHGKYSEFLKQKSHLREDYIRRYQAQQKKIEKEETFIRKN
                     KAGVNSKIARGRQKQLDKIERIAPPSFTGKPNIQFSEIEISAQNALTITNLEVGYYYS
                     LLPKLNFSVDGGQKIVITGFNGIGKSTLLKTLVKDIPRISGDFQFSEQVKIGYYEQDL
                     KWENPDKTPLQIVADKYPKLNTKEIRRHLARCGVKEEHVSRSVSTLSGGEQSKVKLCC
                     MMLSPCNFLILDEPTNHFDAETKDALQNALKQFRGSIILVSHEEKFYKGWIDKVFNIE
                     KQLV"
     gene            2917..3042
                     /locus_tag="ACOIAF_003788"
     CDS             2917..3042
                     /locus_tag="ACOIAF_003788"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670763.1"
                     /translation="MEIKKAEEWHCKMPTSAFGKTKGHRQALLSMTRKSILSIGK"
BASE COUNT         1125 a          439 c          459 g         1186 t
ORIGIN      
        1 taattgatat aaagaattaa taaaaataac atataaaagt ttaaaaggga gttgaactta
       61 aagtttgatt agaaaaagtc aattttaaga caactccttt tattttacta ttgtattaat
      121 ctgtatctgt aagtgtatta gtctaatatt ataaactttg gatgaaatta attttctata
      181 ggttttaatg tatcatacac tttattttaa agaaacttat cttaattttc atagtgattg
      241 aatttgtcta ttagagatgt tactattagt aatgtaaaag caagagtagt gaatataaaa
      301 aacaagatag tgatactgca agattagaat ataataaatt tttatggaga gatttcaata
      361 tggatacatg ttttaataaa aaaatattac ttgttgatga tgaaaaggat atagtagatt
      421 taattgaaga agtactcata aatgatggtt ttaaaaacat tattaaagct tataatggtc
      481 tagatgctat ttcgctgtgt aagatagcat gtccagatgt tgtcattctt gatataatgt
      541 taccagatat agatggtata gaagtgtgta aaaaaattcg agagttttca tactgttcta
      601 ttcttttttt atcatcaaaa aatgatgata ttgacaaaat actaggtctt agtagtgggg
      661 gagatgacta tataacaaag ccattcagtc caagagaaat tgcttttcga gttaaagcgc
      721 aattacgtag acaacagtat caaagcattg taccatcaga tagtgaagtt attaaaatag
      781 gagatattac tattgatata gaaggaaacc gtgtatacaa agatagaaat gagattgagt
      841 taactggaag ggagtatcat cttttaagct acatggctca aaatgtaaat aaaattatag
      901 gaaaagaaag actctatgaa caagtatggg gagtatatag tagtatttgt gacaatacaa
      961 ttatggtgca tatacgacat ataagagaaa aaattgaaga taacccctca aatcctaaaa
     1021 tacttataac agttaaagga ctaggatata aacttgtgaa tagaatcgat tagaatagat
     1081 tttaatatta atgaagatat ctcaagttga atttttagtt aatgaggcat tcttttatgg
     1141 gtgaaatcaa atgtattttc gttatctcaa caatgaagaa gaggtctatc tctattttga
     1201 gatagcctca ttaagtttat atcctggtaa tgtactttat gttctaataa aattttttta
     1261 gtttatttgc agtgaaacat taactagaac attaaaattt gcattgaaat accaattaaa
     1321 taatattgta tattgtgtat aaacttatac taattgtttt tctatattaa atactttatc
     1381 aatccaacct ttataaaatt tttcttcatg ggaaactagg attatacttc ctctaaactg
     1441 tttcaaagca ttttgtaaag catcctttgt ctctgcatca aagtgattgg ttggttcatc
     1501 cagaataaga aaattacaag gagataacat catacaacat aatttaacct tagattgttc
     1561 accaccactt aaagtagata cactccttga aacatgctct tccttcacac cacatctggc
     1621 tagatgacgt ctaatctctt ttgtatttaa ttttggatat ttatctgcta ctatttgaag
     1681 tggagtttta tcaggatttt cccattttaa atcttgctca taataaccaa ttttaacttg
     1741 ttctgaaaac tggaaatctc cagatatacg aggaatatct tttactaatg tctttaacaa
     1801 ggtagattta ccgattccat taaaaccagt tataactatt ttttgacctc catcaacaga
     1861 aaaatttaat tttggaagta aagaatagta gtatccaact tcaagatttg ttatagtaag
     1921 agcattttga gccgatattt caatttctga aaattgaata tttggttttc ctgtaaaact
     1981 aggaggagct attctttcta ttttatccaa ttgtttttgt cttcctcttg caatttttga
     2041 atttactcca gctttatttt tacgtataaa tgtttcttct ttttctattt tcttttgttg
     2101 tgcttgatac cttctaatat aatcttctct taaatgtgat ttttgtttaa ggaactctga
     2161 atattttcca tgatatttct taatcatgcc aaattctata tcaagaatac ctgtagaaat
     2221 tttttctaaa aagtcaaaat cgtgggaaac tacaataaat gccccattaa atgtatttaa
     2281 ataatttgag agccattcaa catgttcttt atctaaaaag tttgttggtt cgtctaataa
     2341 tagaacatca tggttagaaa gtagtaattt tgctaaaatt acctttgccc tttgaccacc
     2401 actaagctct ccaacaacac gattcattcc tattgaatct aatccaagac catttgctat
     2461 tttatttatt tcattatcta tagaatagaa attatttgct attagttgtt cctgataatc
     2521 tgatgctttg attagatatt gctcattttc agatatagca cttttttgat agagtagatt
     2581 catttccttt tctattttat aaagttcctc aaatgcagtg tgtaaataga gtgatattgt
     2641 tgtatctcta ttgatttctg catattggtc taaatggcct atattgatat taggttgcca
     2701 cttgatttct cctgaatctg gaacaacttc tcccaataaa attttaatta atgtactttt
     2761 acctgtacca ttttggccaa ctacacctat atgttcacct ttgaataaat caaagttagc
     2821 tttctcatac agtgatttat ccataaaaca ttgtgataaa ttagttactt ttaataaact
     2881 cataattcaa actccttttc ttttattaaa aaatttatgg aaataaaaaa agcagaggaa
     2941 tggcattgta aaatgccgac ctctgctttt ggaaaaacaa aaggtcatag acaagcattg
     3001 ttgtctatga ccagaaaatc tatattatct atcggcaaat aggtaatttt aaataagcat
     3061 agccaaaaaa ctatactaaa cctatatttg ccgatgaaga tagaaagctt taagctttca
     3121 gattaacctc tacacaacgc ttcatcgtat acatttgtgt agagttaagt tttctaacaa
     3181 gtttaactaa ttctatataa atcataatt
//
LOCUS       DBGJFF010000238         3163 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.243, whole genome shotgun sequence.
ACCESSION   DBGJFF010000238 DBGJFF010000000
VERSION     DBGJFF010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3163
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.243"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            <1..1015
                     /locus_tag="ACOIAF_003789"
     CDS             <1..1015
                     /locus_tag="ACOIAF_003789"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HGZ2670764.1"
                     /translation="LNYLIFTSDINTDIYKFDINMGLSSLITIIIAGFIYWYSSKKFN
                     IKVMNFDKYIAILMIPLLLIILFMQSFEYSSNINIDTSLGIVKLLLNTSITEEIQAFL
                     FSIIGTICVFFSLFTFKKLIQALEDDKERAIMNQQIHAQKNYIEEAKSRLSQTISFRH
                     DFNNHLAIVNGLLKKDQILKAQDYLNKLEKVTSDLSFSCNTQNIVIDALFNNKLSIAK
                     QLGIEVDCEVIIPQSTNMNDFDLCIVFANAIDNAIKACRVVDKQNRYLKLSTKLEGGF
                     FMIEIKNSYNPINNYTSGSGIGLLNIKEIAKKYQGAISIDKTSDYFQINVLLVISLHC
                     ASI"
     gene            1105..1539
                     /locus_tag="ACOIAF_003790"
     CDS             1105..1539
                     /locus_tag="ACOIAF_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670765.1"
                     /translation="MKVGNINGQAPITNGLDSCQSEVQGLEQKKKSLQDEIEKVRSDK
                     SIDGETAKLKIEALKKEMEEIENKIQELKGNKAKKTNKEENNVSEVNNNLLERKKEPS
                     NNENVKQLYDVFERSNVDEQWDNTYRVLQDDKNLKVKFNRPI"
     gene            complement(2194..2418)
                     /locus_tag="ACOIAF_003791"
     CDS             complement(2194..2418)
                     /locus_tag="ACOIAF_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HGZ2670766.1"
                     /translation="MTVYNLKLGQKGIIDNIAGNEKLMKRLLALGLIDGTEVEVKKIA
                     PLNDPIVIRFRGFDLAIRKSDAKNINLKNN"
     gene            complement(2515..>3163)
                     /locus_tag="ACOIAF_003792"
     CDS             complement(2515..>3163)
                     /locus_tag="ACOIAF_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HGZ2670767.1"
                     /translation="YNRNLNRFGSKFFRNKNRIKNNNFSKTITPSVFLEQVLLIFICI
                     NTGEIISRCFYITFNILLPSVVTCMFSAVIFRNLNDKINILELNFKLIDFLKELSLGI
                     FLTLSLMNIDLFELSTLLPPILLIVTFQVIFIILFSIFICFRVLGKDFDSAIIISGLI
                     GHGIGATPNALANMSSLTQKYGDSPKAFLVVPLVSGFLLDAISIPCILFFINILT"
BASE COUNT         1260 a          381 c          420 g         1102 t
ORIGIN      
        1 attaaattac ttgatattta ctagtgatat aaatacagat atttataaat ttgatataaa
       61 tatgggacta tcttctttaa ttactattat aatagctgga tttatatatt ggtatagttc
      121 taaaaagttt aatattaagg tcatgaactt tgataaatat atagcaattt tgatgattcc
      181 attgttgctt ataatattat ttatgcaatc atttgaatat agttcaaata taaatataga
      241 tacaagtctt gggattgtaa agctgctatt aaatacttct ataactgaag aaattcaagc
      301 ttttcttttt tctataattg gaactatatg tgtatttttt tctctattta cctttaagaa
      361 actaattcaa gctctcgaag atgacaagga gagggcaatt atgaatcaac agattcatgc
      421 tcaaaaaaac tatattgaag aagcgaaatc acgattatca caaactatat cctttagaca
      481 tgattttaat aatcatttag ctattgtcaa tggattgttg aaaaaagacc aaattttaaa
      541 agcacaagat tatttaaata agcttgaaaa agtaacaagt gacctatcat tttcttgtaa
      601 tacacagaat attgttattg atgctctttt caacaataaa ttaagtatag caaaacaatt
      661 aggcatagag gtagattgtg aagtaattat accccaaagc actaacatga acgattttga
      721 cctatgtata gtgtttgcta atgctattga caatgcaatt aaagcttgta gagttgtaga
      781 taaacaaaat aggtatttaa agctttccac taagttagag ggtggatttt ttatgataga
      841 aataaaaaat agctataatc caataaataa ttatacaagt ggctcaggta taggtctttt
      901 aaatataaaa gaaattgcta aaaaatatca aggggctata agtatagata agacatcaga
      961 ctattttcaa ataaatgtat tgctagttat ttcattacat tgcgctagta tttgagaaca
     1021 atttcattga aaatggtatt tgtaagctat attatgtact tgaatagaga aagtttgata
     1081 tatagaaatg aggaggttac taaaatgaaa gttggaaata taaatggtca agcaccaatt
     1141 acaaatggtt tagatagctg tcaaagtgag gtacaaggct tagaacaaaa gaaaaaatca
     1201 ttacaggatg aaatagagaa agtaagaagt gataaaagta ttgatggtga aacagccaaa
     1261 ttaaaaatag aagctttaaa aaaagaaatg gaagagattg aaaataaaat tcaagagtta
     1321 aaaggaaata aagcaaaaaa aacaaataaa gaagaaaata atgtttcaga agtaaataat
     1381 aatctattag agagaaaaaa agaaccttct aataatgaaa atgtaaaaca attatatgat
     1441 gtttttgaaa gaagcaatgt agacgaacaa tgggataata cttatcgtgt tctccaagat
     1501 gataaaaatt taaaagtaaa atttaataga cctatatagt ttatttgtaa aaatagttaa
     1561 tattttaatt atcaccactt tttagaagaa tacacacttt attttaaaga cccacatatt
     1621 tatttacagt tagcacattt atatgtggtt ttataactat tatttacaag tatatattga
     1681 taacttttac atagtatata atgtttaaat acttattcaa atttatctat acataaacaa
     1741 aaaaagagat tttaaaatct ctttttagat tgttgactta tggctttttg atttaataaa
     1801 atcaagaagc cgtttgaatt ttatctgttg gctgataccc ttctgatatt gaaacaccat
     1861 ttttgtttcc aaaatcgttt aaaattaaga acttattatg aatgactatt tcattatgaa
     1921 caataagcaa atgacctgaa tcttcaatca atgttttaac agtagcatta aatgcttgat
     1981 tatacagttc tatcgtttgt ttgaactgcc cattaaagta aatatgagtc aataacataa
     2041 ctatatctac acaaattaat ataaattttt tatactgtac attatctaac tgttgattat
     2101 ctgtattaca acatgattga atttaaaaag ccatcaacac atagaatgtt gatggcctga
     2161 aaatagattt taaaatctct tttttattat atattaatta ttttttaaat ttatattttt
     2221 tgcatcagat tttctaatag ctaagtcaaa ccctctaaat ctaatcacta tagggtcatt
     2281 aagaggagct atctttttca cttcaacttc agtgccatct attagtccta aggctagaag
     2341 tctcttcata agtttttcat ttccagctat attgtctata attccctttt gtccaagttt
     2401 taagttgtaa acagtcatta ttaacacctc ctaatgaaaa tcattatcaa actttatatt
     2461 tataatatat aacaaaaaga gcaaattgtc aatatataac aaaaaataaa catactaagt
     2521 tagtatgttt ataaaaaata gtatacatgg gatactaata gcatctagca aaaaaccact
     2581 tactaatggt acaaccaaaa aggcttttgg agaatcacca tatttttgtg tcaaagaact
     2641 catatttgcc aaagcgttcg gagtagctcc tattccatgt ccaatcaaac cgcttattat
     2701 gatggcagaa tcaaaatctt ttccaagtac tctaaagcaa ataaatattg aaaatagaat
     2761 tataaagatt acttgaaaag ttactattaa gagaatagga ggtaataatg tagataattc
     2821 aaataaatct atattcataa gtgataaagt taaaaaaatt cccaatgaaa gttcttttaa
     2881 aaaatctata agtttaaaat ttaattctag tatatttatc ttatcattta aatttctaaa
     2941 aataacagcc gaaaacatac aagttacaac agagggtaat agtatattaa acgttatata
     3001 gaagcatcta cttataattt ctcctgtatt tatacaaata aatatgagaa gaacctgttc
     3061 caaaaataca gatggtgtta ttgttttgga gaagttatta ttttttatcc tattcttatt
     3121 tctaaaaaat ttacttccaa atctatttag atttctatta tat
//
LOCUS       DBGJFF010000239         3141 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.244, whole genome shotgun sequence.
ACCESSION   DBGJFF010000239 DBGJFF010000000
VERSION     DBGJFF010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3141
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.244"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(<1..266)
                     /locus_tag="ACOIAF_003793"
     CDS             complement(<1..266)
                     /locus_tag="ACOIAF_003793"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGZ2670768.1"
                     /translation="MKKILVACGAGIATSTIVCDRVERLVKENNIQAEVEQCKIAECS
                     TKQEGADLIVSTTILPTTYDIPTIKATGYITGINTTALDKKILNA"
     gene            complement(385..957)
                     /gene="mobB"
                     /locus_tag="ACOIAF_003794"
     CDS             complement(385..957)
                     /gene="mobB"
                     /locus_tag="ACOIAF_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HGZ2670769.1"
                     /translation="MLLLDSFMKGHNFMYNILSVVSYSGVGKTTLIEGIIKELNKKGY
                     RVGVIKHTCHDFDMDEEGKDTYKHRKSGARKVCIISDKRVAYIEELKEKNPLYKMIQL
                     YEDMDLIIIEGYKNYRFKRLEVTRKGKYEDILSNKSDLIGIVSDIEYKLNIEQFNLND
                     YKNISKYIESCIKNEILSISNVEIKNILKE"
     gene            complement(941..2122)
                     /locus_tag="ACOIAF_003795"
     CDS             complement(941..2122)
                     /locus_tag="ACOIAF_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HGZ2670770.1"
                     /translation="MIELEDAIKIIEKNVLPINRVKIVNLIDAVNKVVAEDIYSTIDS
                     PPFDRSPYDGYAYNANSENRNLKVIGTLYAGDVFNRVLNKNEAVKIMTGGKIPQGANC
                     VIKKEDVTVIGDMITLNIKLKEYDNYIFKGEDIKKGQLIINKNQNIGYDGIGVLASLG
                     ISKVTVYDDLKVGILNTGTELQDINETLENGKIYDSNRYTLYSRLLKLGIQADTICNC
                     SDDILDLEKKVKNMLQSVDFLITTGGVSVGDKDLIPDVFKKIGATELFWKINIKPGGA
                     CYVGTLGEKLLFGLSGNPHAAIVVFDNVIVPVIEYLTYKKGTHHIKAIFKGEFNKLSN
                     KDRFVSGKLYTSEGKLYVELNDKKDAKARLSATLRSNCMIKIKKNETIKENQLVDVVI
                     R"
     gene            complement(2333..3067)
                     /locus_tag="ACOIAF_003796"
     CDS             complement(2333..3067)
                     /locus_tag="ACOIAF_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670771.1"
                     /translation="MKLKRSLVCVTILGIILVGCHKENTKEKNQVASKATQQKTMTKV
                     QNDVNEIMNKDYKYIIKNMGIPYNTFYYIKPKVLKESNTMQDINTSSYMTLVYPKYTG
                     NDELDGSALYVDINENKVVNVETNSFSSQGISVIDAESSIVIEKSDHEKSAVSLENFR
                     HIDLGEYVGVEDSRINEIVGDANYDLTAYNHEGSKVVKSYRLKEDNKILKKEVLTISI
                     VDNKIKSIKTIESDKIVKIIKGTLLE"
BASE COUNT          970 a          554 c          263 g         1354 t
ORIGIN      
        1 gcatttaata tctttttatc taatgctgtt gtatttatac ctgttatata tcctgttgcc
       61 tttattgtag gtatatcata agttgttggt agtatagtag tagatacgat taaatcagca
      121 ccttcttgtt ttgttgaaca ttcagcaatt ttacattgct caacttctgc ttgtatatta
      181 ttttctttaa ccaatctttc tactctgtca caaactattg ttgatgttgc gattcctgct
      241 ccacaggcta ctaatatttt tttcatatat tgtcctccta caatttcttt aatattttat
      301 aactcaaaat aaaattgata aatttcttat atctttattt atataaataa ttatatcatt
      361 ttatataaga agaacatctt aaaattattc ttttaatatg ttttttattt caacattact
      421 tatacttaaa atttcatttt ttatacaaga ctctatgtac tttgatatat ttttataatc
      481 atttaaatta aactgttcaa tattcaattt atattctata tcactgacaa tacctatcaa
      541 atcagactta ttagataaaa tatcctcata cttacctttt ctagttactt ccaatctttt
      601 aaatctataa tttttatatc cttctatgat aatcaagtcc atatcttcat ataattgaat
      661 cattttatat aatggattct tctcttttag ttcttcaata tatgctactc gtttatctga
      721 tattatacag acttttctcg ccccagattt tctatgttta taagtatctt ttccttcctc
      781 atccatatca aaatcatgac aggtatgttt aataactcca acacgatacc cttttttatt
      841 taattctttt attatgcctt caatcaacgt agttttacct actcctgaat atgagacaac
      901 tgacaatata ttatacataa aattgtgtcc tttcataaaa ctatctaata acaacatcca
      961 ctaattgatt ttcttttatt gtctcattct ttttaatctt aatcatacaa ttacttctta
     1021 gagttgcaga tagtctagct tttgcatctt tcttatcatt tagctctaca tacaatttac
     1081 cttcacttgt atatagtttt ccacttacaa atctatcttt atttgaaagt ttattaaatt
     1141 ctcctttaaa tatagctttt atatgatgtg ttcccttctt atatgttaaa tactcaataa
     1201 caggaactat cacattatca aataccacta ttgctgcatg tggattacca gaaagaccaa
     1261 ataatagctt ttctcccaat gtacctacat aacaagctcc accaggttta atgtttatct
     1321 tccaaaataa ttctgttgcc cctatttttt tgaatacatc tggaattaaa tctttatctc
     1381 caacagaaac accacctgtg gtaattaaaa aatctacact ctgtaacata ttctttacct
     1441 ttttttctaa atccaatata tcatcagaac aattgcatat tgtatctgct tgtattccaa
     1501 gttttaataa tcttgaatat aaggtatatc tattactatc gtatatttta ccattctcta
     1561 aagtctcatt tatatcttgt agttctgtac ctgtattcaa aattccaact tttaaatcat
     1621 cataaacagt tactttggat attccaagac ttgctaatac tccaatacca tcatatccta
     1681 tattctgatt tttattaata atcaattgcc ccttttttat atcctcaccc ttaaatatgt
     1741 aattatcgta ttcctttaat tttatattta aagttatcat atcacctata actgttacat
     1801 cttctttttt tattacacaa tttgcaccct gtggtatctt tccaccagtc attatcttca
     1861 ctgcttcatt tttatttaat accctattaa atacatctcc tgcatacaaa gtccctataa
     1921 cctttaaatt tctattttca gaatttgcat tatatgcata accatcatag ggtgacctat
     1981 caaatggagg actatcaatt gttgaatata tatcttctgc aactacctta tttactgcat
     2041 ctataagatt tactatcttg accctgttta ttggtaaaac atttttttca ataattttta
     2101 tggcatcttc taattctatc attttaaata ctccccaata tattttttat ttaagatatt
     2161 tctatgtaat atattataga ttataaaagt tggaaatact tttatcttat atttaattat
     2221 aactaatagt tatctataag tgcagttcaa attttataaa tctaataaaa aaactgttcc
     2281 cagctagaaa tttccttcta tcttgaaaca gcatcatatg ctttagaata ttttattcta
     2341 ataaagttcc ttttattatt tttacaattt tatcactttc aatagttttt atagatttta
     2401 ttttgttatc cacaatactt attgtaagta cctctttttt taatattttg ttatcttctt
     2461 tcaatctata actcttaact actttactgc cttcatgatt atatgctgtt aaatcataat
     2521 tcgcatctcc tacaatttca tttattctgg aatcttcaac accaacatac tctcccaggt
     2581 caatatgtct aaaattttct agtgatacag ccgatttttc atggtcactt ttttcaatca
     2641 ctatactaga ttcagcatca atgactgata taccttgtga tgagaaagaa ttggtttcaa
     2701 cattgacaac tttattttcg ttaatatcaa catataaagc acttccatct agttcatcat
     2761 ttccagtata ttttggataa actaatgtca tataacttga tgtatttata tcttgcattg
     2821 tatttgattc ttttaaaact ttaggtttta tataataaaa tgtattataa ggtataccca
     2881 tattctttat aatgtactta taatctttat tcataatttc attaacatca ttttgaactt
     2941 ttgtcatagt tttttgctgt gttgctttac ttgctacttg atttttctct ttagtatttt
     3001 ctttatgaca tccaacaagt ataataccta aaatagttac acaaactaat gaccttttta
     3061 acttcataaa tcaatcctcc tttcttaaat tttatagtat ttatctatat ctcaatttac
     3121 agttatctct tttttcaata a
//
LOCUS       DBGJFF010000240         3133 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.245, whole genome shotgun sequence.
ACCESSION   DBGJFF010000240 DBGJFF010000000
VERSION     DBGJFF010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.245"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            241..2883
                     /gene="adhE"
                     /locus_tag="ACOIAF_003797"
                     /gene_synonym="adhC"
     CDS             241..2883
                     /gene="adhE"
                     /locus_tag="ACOIAF_003797"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HGZ2670772.1"
                     /translation="MEKKEKVVEKSNVEVCSPEIVNSVETLRMRLEEIRLAQKEFATF
                     TQEQVDKIFLAASTAANQQRIPLAKMAVEETGMGIVEDKVIKNHFASEYIYNAYKDTK
                     TCGVIEKDEAFGFTRIAEPVGVLSAVIPTTNPTSTAIFKSLIALKTRNGIIFSPHPRA
                     KNCTIEAARVVHDAAVKAGAPKGLIGWVDVPSIELTNVVMAEADLILATGGPGMVKSA
                     YSSGKPAVGVGPGNVPAIIDESADIKMAVSSILVSKSFDNGMICASEQAVIVPEKIYE
                     EVKKEFKYRGAHFLNKEETEKVGKVVIIDGSLNARIVGQPAHVIAKMADVEVPKTARI
                     IIGEVESVELNEPFAHEKLSPVLAMYKSKSFEDAVAKAEKLVADGGYGHTSSLYADSI
                     NHPERVEKFVNAMKTCRVLVNTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVSENVG
                     VKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVAAREFKDVMDKKKAFIVTDSFLY
                     NNGYTKKLTDLLDEMGIKHTTFFDVAPDPTLACAREGAKAMADFQPDLIIAVGGGSAM
                     DAGKIMWVMYEHPEVDFQDLAMRFMDIRKRVYVFPKMGEKAYFAAIPTSAGTGSEVTP
                     FAVITDQDSGVKYPLADYELMPNMAIIDADMMMEMPPRLTAASGVDALTHALEAYVSM
                     LRTEPADGLALQAGKIIFEYLPRAYKNGKNDKEAREKMAMASTMAGMSFANAFLGICH
                     SLAHKLGAFHHVQHGVANALLINEVIKFNCAEAPNKMGAFSQYRYPDCIQRYAEFASF
                     AGIKGSTDQEKVDNLIKAIDELKAKVGLPKTIKEAGVEESKFLERLDAMVEQAFDDQC
                     TGANPRYPLMSELKEIYLKVYYGK"
BASE COUNT         1200 a          399 c          610 g          924 t
ORIGIN      
        1 atatatctca tttagataaa aaattttagt aaaagtaaaa aaaataatat atttgttaaa
       61 aaataaattg acaaaatatt cagaaaaaaa cttcttgacc taagagagta aaataaataa
      121 aatctaaggt atagagatga aatctataaa attattttaa gtataagtta aaaatataga
      181 tgatgcattt aagatttata aaaattagag atgcataatt attttaggga ggtttttagt
      241 atggagaaaa aagaaaaggt agtagaaaaa tcaaatgttg aagtatgtag tcctgaaatt
      301 gtcaacagtg ttgaaacatt aagaatgaga ttagaagaaa ttagattggc acaaaaagaa
      361 tttgcaacat ttacgcaaga acaggtagat aagattttct tagctgcttc aactgcagct
      421 aatcaacaaa gaatacctct agcaaaaatg gcagtggaag agactggtat gggtattgtt
      481 gaagataagg ttataaaaaa ccattttgcc tcagaatata tctataatgc ttataaagat
      541 actaaaactt gtggtgtcat agaaaaagat gaagcatttg gttttacaag aattgctgag
      601 ccagtaggtg ttttatcagc agttatacca acaactaatc ctacatcaac tgcaatattc
      661 aaatccctaa tagcactaaa aactagaaat ggtataattt tctctcctca tccaagagct
      721 aaaaactgta ctatagaagc agctagagtt gtacatgatg cagcagttaa ggcaggagca
      781 ccaaaaggac ttataggttg ggtagatgtt ccttcgattg aattaactaa tgttgtcatg
      841 gcagaggcag accttatatt agctactggt ggacctggaa tggttaaatc tgcatactct
      901 tcaggtaaac cagctgtcgg agttgggcct ggaaatgtac ctgcaataat agatgagagt
      961 gcagatataa aaatggctgt aagttcaatc ttagtatcaa aatcattcga taatggtatg
     1021 atatgtgctt ccgagcaagc tgtaatagta ccagaaaaaa tatatgaaga agttaaaaaa
     1081 gaatttaaat atcgtggagc acatttccta aataaagaag aaactgaaaa agtaggaaaa
     1141 gtagtaataa ttgatggttc attaaatgcc agaatagttg gacaaccagc acatgtaata
     1201 gccaaaatgg cggatgtaga agttccaaaa acagctagaa taataattgg tgaagtagaa
     1261 tctgttgaat taaatgaacc ttttgcacat gaaaaacttt ctcctgtact tgcaatgtac
     1321 aagtctaaaa gttttgaaga tgcagttgct aaagcagaaa aattagttgc agatggaggc
     1381 tatggtcata catcttcatt atatgcagac tctataaatc atccagagag agtagaaaag
     1441 tttgtaaatg ctatgaagac ttgtagagta ttagttaata ctccatcatc acaaggtggt
     1501 ataggtgatt tatataactt taaattggca ccgtctctta ctcttggttg tggttcttgg
     1561 ggtggaaact cagtatctga aaacgttgga gtaaaacatt tgcttaatat caagacagta
     1621 gctgaaagga gagagaatat gctttggttc agagcaccag aaaaggttta tttcaaaaaa
     1681 ggatgtttag gtgtagcagc aagagaattt aaggatgtta tggataaaaa gaaagcattt
     1741 atagtaacag attctttcct atataataat ggatatacta agaaattaac tgatttatta
     1801 gatgagatgg gaattaagca tactacattc tttgatgtag ctccagaccc aacattagct
     1861 tgtgcaagag aaggagctaa agctatggct gatttccaac cagatttaat aatagctgta
     1921 ggtggaggaa gtgcaatgga tgctggtaag ataatgtggg taatgtatga gcatccagaa
     1981 gtagatttcc aagacttagc tatgagattt atggatataa gaaaaagagt atatgtattc
     2041 cctaagatgg gtgaaaaagc gtattttgct gctatcccaa cttcagcagg aactggttca
     2101 gaagtaactc catttgcagt tataactgac caagattcag gtgtaaagta cccattagca
     2161 gattatgaat taatgccaaa catggctata attgatgctg atatgatgat ggagatgcca
     2221 ccaagattaa cagcagcatc aggtgttgat gctttaacac atgcattaga agcatatgta
     2281 tctatgttaa gaactgaacc agctgacggt ctagcactac aagcaggtaa aataatattt
     2341 gaatatttac ctcgtgcata taaaaatgga aagaatgata aagaagcaag agaaaaaatg
     2401 gcaatggcat ctactatggc aggaatgtct tttgcaaatg ccttcttagg aatatgtcac
     2461 tctttagcac ataaattagg tgcattccac catgttcaac acggtgttgc aaatgcatta
     2521 ttaataaatg aagtaatcaa gttcaattgt gcagaagctc caaataaaat gggtgcattc
     2581 tcacaataca gatatcctga ttgcatacaa agatatgctg aatttgcttc ttttgcaggt
     2641 attaaaggaa gtacagacca agaaaaagta gataatctta taaaagcaat agatgaacta
     2701 aaggcaaaag taggtcttcc taagactata aaagaggctg gagtagaaga atctaaattc
     2761 ttagaaagat tggatgctat ggttgaacaa gcttttgatg accaatgtac aggtgctaat
     2821 cctagatacc cattgatgag tgaattaaaa gagatatatc taaaagttta ttatggtaaa
     2881 taatatctat tgtgcaacat aggttctatt gggtttgtat ataaattatt agatttgaat
     2941 ataaatagag ttttattaga gatatgtata aatagttata aataatagat tataacttaa
     3001 attctgaatg gaaatgataa aaggtcagta atttaaagat aattttgaat atgtgtattt
     3061 aatcaattaa ctttaaaatt agtcggaaaa tttttttatt acatataatt ttaaagcata
     3121 atatataaaa aac
//
LOCUS       DBGJFF010000241         3083 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.246, whole genome shotgun sequence.
ACCESSION   DBGJFF010000241 DBGJFF010000000
VERSION     DBGJFF010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3083
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.246"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            237..503
                     /gene="rpsT"
                     /locus_tag="ACOIAF_003798"
     CDS             237..503
                     /gene="rpsT"
                     /locus_tag="ACOIAF_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HGZ2670773.1"
                     /translation="MANIKSAKKRISVIEKKTALNRVRKSQIKTAIRRFEDAVAAGNR
                     EDAVAKFQYAQKRIYQVASKGTIHKNAAARKVAKLAQKLNGMNA"
     gene            complement(690..1733)
                     /gene="holA"
                     /locus_tag="ACOIAF_003799"
     CDS             complement(690..1733)
                     /gene="holA"
                     /locus_tag="ACOIAF_003799"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HGZ2670774.1"
                     /translation="MNYKDIIRNVKEKNFEKMYLFYGREYYLIENAIKAFKDSLNEGM
                     LDFNLDIIDGKEIVLNQLISSIETLPFMDDRKIVIIKDFELLKGKKKNFTDSDEKYLI
                     EHLDNIPDTTTIVFVVYGDVDKRKSLVKKIGNNGIVFDCDKLSDMDLFKWIKKSFALN
                     DVIIDNSQIMYFIEQEGYRDKSSEKTLSDLENEINKISSFVGKGNNVTNDVIDKLSQK
                     KVENDIFKLIDYIGEQNASNAMKILNDMIQEGESVLGIFSMIARQFKIIMQVRQLQLD
                     GYSTKLIADKLKMHQFVVGKALKQTKNFSDDIIVEILNYILESDYKIKTGLIRDTLAV
                     EMLVSRYCKREAI"
     gene            complement(1687..2988)
                     /locus_tag="ACOIAF_003800"
     CDS             complement(1687..2988)
                     /locus_tag="ACOIAF_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HGZ2670775.1"
                     /translation="MGVIIIQIRRPLLIILMFVVAISFIITNNTKNDEELNDKIITIQ
                     GVVKGKIEKKRYNQYKVGVFLINDYTKNKNLKIGQKVNITGKFKSLDKMKYDDFDYGR
                     YIKSTGYKGIVYINNYKIIDKNKLYSLIGEIKFYISKTYRYLYKKNSDFINSILLAEV
                     ENLTEEQKEIFTRTGTSHVISISGLHTGILCVIISFLLRGINKLYKLLILSIFITLYC
                     IMVGASPSIIRSIAFVMIFYLSIFIDRKKDGISALSLIGIILIMNNPYVIYNVSFQLS
                     FLATLSILIFYNKINSIIKLSMVSLTISSNILTLPIIYYTFKGIPLLSIIGNLIIVPF
                     VGVIMYLSIASLIVFKVSVVIAKIISFFNSTLIESIFFLLEKISNLSFAYINIESPKF
                     YIVVIYYIGVFFYIFYIEGKEIKEQENELQGYYKECKREKL"
BASE COUNT         1138 a          445 c          285 g         1215 t
ORIGIN      
        1 tttgttacaa aaataaaata ttaatttata tatgtcaaaa tatttaaaac acaaaatata
       61 ccaatttaaa aacaagatat tgcaaattat ttatacacaa tttacagtct aaaaaatcaa
      121 aataaaaaac attattaaaa tatataaata ataatccttg atttttagat ttatttctgt
      181 taaaatctaa tatgttgacg atgaatatga taaaaaaata cagggaggtg aacacggtgg
      241 caaatataaa atcagctaag aaaagaataa gtgttatcga aaagaaaact gctttaaata
      301 gagttagaaa atctcaaata aaaactgcaa taagaagatt cgaagatgct gttgcagctg
      361 gaaacagaga ggatgctgtt gctaagttcc aatacgctca aaaaagaata taccaagtag
      421 cttctaaagg aactatacat aagaatgctg cagctagaaa agtagctaaa ttagctcaaa
      481 aattaaacgg tatgaacgct taattaccag tttatatttc ttatatataa taacccctat
      541 gtaggggttt tttattttgt aaatttatac aataaaaaat aaatagttag acatattata
      601 gttgattgta tatcaaactt aatttagctt acatctactc tgaactataa catatcttgc
      661 tatttatttt ttattaatat atagattttt taaattgctt ctcttttaca gtatctacta
      721 accaacattt caactgctaa agtatctctt ataagacctg tctttatttt gtaatcactt
      781 tctaatatat agtttaatat ttccacaatt atatcatcag aaaaattttt agtttgcttt
      841 aatgcttttc ctacaacaaa ttgatgcatt tttaatttat cagctataag ttttgttgaa
      901 tatccatcaa gttgtaactg tcttacttgc ataattattt taaattgtct tgctatcata
      961 gaaaatatac ctaatacaga ttcgccttct tgtatcatat catttaatat tttcattgca
     1021 ttagaagcat tttgttctcc aatataatcg attaatttga atatatcatt ctcaactttt
     1081 ttttgagata acttatcaat tacatcattg gttacattat tccctttacc aacaaatgaa
     1141 cttattttat ttatttcatt ttctaaatca gataatgtct tttcactact cttatctctg
     1201 tatccttctt gttcaataaa atacatgatt tgagaattat ctattataac atcatttaaa
     1261 gcaaaagatt tttttatcca tttaaacaaa tccatatcag aaagcttatc acaatcaaaa
     1321 acaattccat tgttccctat tttcttaact aatgattttc ttttgtctac atctccatat
     1381 acaacaaaaa ctatagttgt ggtgtctgga atattgtcta aatgctctat taagtatttc
     1441 tcatcactat ctgtaaaatt ctttttcttt ccttttaata gttcaaagtc ttttattatt
     1501 actatctttc tatcatccat aaatggaagt gtttctatag agcttataag ctgatttaag
     1561 actatttcct tcccatctat tatatctaag ttaaaatcaa gcattccctc gtttagacta
     1621 tctttaaatg cttttattgc attctcaatt aaataatact ctcttccata aaaaagatac
     1681 attttttcaa agtttttctc ttttacattc cttataatat ccttgtaatt cattctcttg
     1741 ctcctttatc tcttttcctt ctatgtaaaa tatgtaaaaa aacactccta tgtaataaat
     1801 aacaacaata taaaatttag gactttctat attaatgtat gcaaaactta aattgctgat
     1861 tttttctagt aaaaaaaata tgctttctat caatgtacta ttaaaaaaag atataatttt
     1921 agctattact acacttactt taaatacgat taaacttgca atacttaaat acattattac
     1981 tcccacaaat ggtactataa ttaaatttcc tataattgaa agcaatggta tacctttaaa
     2041 tgtataataa attataggta aagttaatat atttgatgat attgtcaatg ataccatact
     2101 taattttata atactattta ttttattata gaatattaat atagaaagtg tagctaaaaa
     2161 agacaattga aaacttacat tatatataac ataggggtta ttcataatta aaattatccc
     2221 aattagagac aatgccgata ttccatcttt ctttctatca ataaatattg ataaataaaa
     2281 aatcataaca aatgctatag acctaattat ggatggagac gcacctacca ttatacaata
     2341 taaagttata aaaatcgaca gtattaataa tttatacaat ttatttatac ctcttaatag
     2401 aaaggatata attacacata atattcctgt atgaagacct gatatagata ttacatgact
     2461 agttccagtt ctagtaaaaa tttctttttg ttcctcagtc aaattttcaa cttctgctaa
     2521 tagtatagaa tttataaaat ctgagttttt cttatataag tacctataag ttttactaat
     2581 ataaaacttt atttccccta ttaaagagta aagtttattt ttatcaataa ttttatagtt
     2641 atttatatac actatacctt tatatccagt actttttata taccttccat aatcaaaatc
     2701 atcatacttc attttatcta aacttttgaa tttacctgta atattaactt tctgaccaat
     2761 cttcaaattt ttattttttg tataatcatt tattagaaaa acccctacct tgtactgatt
     2821 gtatcttttc ttttcaattt ttcctttgac aactccttgt atagttatta tcttatcatt
     2881 aagttcttcg tcgttttttg tgttatttgt aatgataaat gatatagcta ccacaaacat
     2941 taatataatt aataatggtc ttcttatttg aattataatc actcccatat aaaagtatat
     3001 tatttagatt attttcaaat agaaaatttt taaacaagat aaattgttat ctaaacaaaa
     3061 aaatacgtca aaagattttt aaa
//
LOCUS       DBGJFF010000242         3071 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.247, whole genome shotgun sequence.
ACCESSION   DBGJFF010000242 DBGJFF010000000
VERSION     DBGJFF010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.247"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            98..1942
                     /locus_tag="ACOIAF_003801"
     CDS             98..1942
                     /locus_tag="ACOIAF_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HGZ2670776.1"
                     /translation="MQKRILRNFIFTGKNILSKLVFIIKYNFSTLILFELFHKGLALF
                     FIWSSIRYIFDILIKSLHIVYLNMDNFIKVLTNPMSIVLIILSVIILAFYAFFEFTSV
                     IICFNKSIKYEKIGLFELFKISFENSIKLLYPKNILLFIFVILIIPLVNTVLISGFIG
                     KIQIPEYILDYIKSDIILNIIYMIFLLIIYISVIRWIFSIHEITLNTENFKRARKESV
                     NLIKGKIIRTIIYSLILSLVVAIIGYAIYHSGIIFIGIWTKYCTDMEFLKNVFINRCI
                     AFEEYFRLILSIFIFILDIGFISATYYQYKGVNISKNNIKKEKKSISKIVFNLVLILL
                     ISYIESVAFSSHINGMFNTSFSFYTTAIAHRAGAEMAPENTLAAIEEASNAKADYAEI
                     DVQETKDGELIVLHDSNFKRTTGVDKNVWDVNYNEVKTYDAGSFYYYGKGYYDEKFVG
                     EKIPTLKEMIKYSKGKVKLLIEIKLNGHEKGDVVKKVVQLIKENNFENQCIVASMDKT
                     ILQKVKKLDENLLTCYFMAIAYGDFSKLNYVDIYGIESTFVNKTVVEKIHKMNKKIFV
                     WTVNSDSLIEKMLDLNVDSIITDNPYLIESAIYWKKNGFISQVSDYLF"
     gene            2264..2848
                     /locus_tag="ACOIAF_003802"
     CDS             2264..2848
                     /locus_tag="ACOIAF_003802"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HGZ2670777.1"
                     /translation="MTEREKMLAGELYDCGDIELLTQWHKAKDLVRDYNQTDSSDLEK
                     KEKILNELLGGKGKNLWITAPYYVDYGNNIYFGNNCEVNMNCTFLDDNKIIIGDNALI
                     APNVQIYTAFHPTNAGDRFGNAKEDGSFEFCKTQTAPVTIGDNVWIGGGAIIMPGVTI
                     GDNVVIGAGSIVTKDIPSNMIAYGNPCRIIRENK"
BASE COUNT         1208 a          284 c          458 g         1121 t
ORIGIN      
        1 catttataca tcataacttg gaataataca tattaatgtt ctaaatgtat ttaaaatata
       61 tgataaaatt agaacaaata gatacaagga gataaaaatg caaaagagaa ttttaagaaa
      121 ttttatattt acgggtaaaa atattttaag taaacttgtt tttataataa aatataattt
      181 tagtacatta attttatttg aattatttca taaaggatta gccttatttt ttatatggtc
      241 atctattaga tatatatttg atattttaat aaaaagttta catatagtct acctaaatat
      301 ggataatttt ataaaagttt tgaccaatcc catgtcaata gttctgataa tactatcagt
      361 tataatacta gctttttatg cattttttga atttacctct gtaattattt gttttaataa
      421 atcgataaaa tatgaaaaaa tagggttatt tgaactcttt aaaatatcat ttgagaactc
      481 tataaaattg ttatatccta aaaatatact attatttata tttgtgatat taataattcc
      541 attagtaaat acagttttaa tttcagggtt tataggaaaa atacaaatac cagagtacat
      601 attagattat ataaagtctg atataatttt aaatataatc tatatgattt ttcttttaat
      661 aatatatatt tcggttatta gatggatttt tagcattcat gagataactt taaatacaga
      721 aaattttaaa agagcaagaa aagaaagtgt taatcttata aagggaaaga ttataagaac
      781 aattatttat tcattaatat tatctttagt agtagctatt attggatatg cgatttatca
      841 tagtggaatt atatttatag gcatatggac taaatattgt actgatatgg aatttttgaa
      901 gaatgtattt ataaatagat gtattgcatt tgaagaatat tttagattga ttttatcaat
      961 attcattttt atattggaca ttggatttat ctcagcaact tattatcaat ataaaggagt
     1021 aaatatttct aaaaataata ttaaaaaaga gaaaaaatct atatcaaaaa ttgtttttaa
     1081 tttagtatta atattattga tttcttatat agagtcagtt gcattttctt cacatataaa
     1141 tggtatgttt aatacgtcat tttcatttta tacaacagca attgctcata gagcaggagc
     1201 tgaaatggct ccagagaata ctttggcagc aatagaagaa gcttcaaatg ctaaagctga
     1261 ttatgcagaa atagatgttc aagaaactaa agatggagaa cttattgtac ttcatgattc
     1321 aaactttaag agaactacag gagtagataa aaatgtttgg gatgtaaatt ataatgaagt
     1381 aaagacatat gatgcgggaa gcttttatta ttatggaaaa ggttattatg atgaaaagtt
     1441 tgttggggaa aagataccaa cactcaaaga aatgataaaa tattctaaag gtaaggtgaa
     1501 attattaata gaaataaaac ttaatggaca tgaaaaggga gacgtagtaa aaaaagttgt
     1561 tcagttaatt aaagaaaata attttgaaaa tcaatgtata gttgcatcta tggataaaac
     1621 tatattacaa aaagtaaaaa aactggatga aaatttactg acatgctatt ttatggcaat
     1681 tgcatatggt gatttttcaa agttaaatta tgttgatata tatggaattg aatcaacttt
     1741 tgttaataaa acagtagtag aaaagattca taaaatgaac aaaaaaatct tcgtttggac
     1801 tgttaatagt gacagtttaa ttgagaaaat gcttgaccta aatgtagata gtattattac
     1861 agataatcca taccttatag agagtgcaat ttattggaag aaaaatggat ttattagtca
     1921 agtgtcagat tatttatttt aaatattcta taattgtatt atctagtaaa agagatatta
     1981 ttaaaatcaa taatatctct ttttaacata aataaggttg ttaaattaag gttattctta
     2041 taaaaattgt gcttatcagc tataaacaag gaataaaagt gaaaaaattg ctctgagatt
     2101 attacagtta aaagagtttt aagatgacag agagatagat tgtcaactgt atatttaata
     2161 aatcttgtta tttattaaat tttatataag taaaatatat aaattaataa atcttatgct
     2221 agaatatata tgaaaaacaa tataaatttg gaggattaaa aacatgacag agcgagaaaa
     2281 aatgttggca ggagaacttt acgattgtgg tgatattgag ttgttaactc aatggcataa
     2341 agcaaaagac ctagtaagag attataacca aacagattcc tcggatttgg aaaaaaaaga
     2401 aaaaatatta aatgaattac ttggtggtaa gggtaaaaac ttatggatta ctgcaccgta
     2461 ttatgtagat tatggaaata atatttattt tggaaataat tgtgaagtca atatgaattg
     2521 tacgttttta gatgataata aaattatcat tggggataat gcactaatcg caccaaacgt
     2581 acaaatatat actgcatttc atccaactaa tgctggtgat agatttggta atgccaaaga
     2641 agatggttca tttgaatttt gtaaaactca aacagctcca gttactattg gagataatgt
     2701 ttggattggc gggggtgcta ttatcatgcc aggtgttact attggagata atgtagttat
     2761 aggtgcagga agtattgtta caaaagatat tccaagtaat atgattgcat acggaaatcc
     2821 atgccgtatt attcgagaaa ataaataaag ttaaattttc ttgcaaaatc tcaaaaaata
     2881 cctttcttac tttatacata gggtattttc taatactttc acatccatat tcatttgtta
     2941 aatttttatg gaatgtagtt tttatcatct ctatcttaca ttttcactat ttttttataa
     3001 aagtcattcc tttatagatt tatttataat agtatatatt ctatttgatg tttgaataag
     3061 cacttatttt t
//
LOCUS       DBGJFF010000243         3060 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.248, whole genome shotgun sequence.
ACCESSION   DBGJFF010000243 DBGJFF010000000
VERSION     DBGJFF010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.248"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     repeat_region   60..1138
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=126..154
                     /rpt_unit_seq="atttacataccacatagttaatataaaac"
     gene            1785..3056
                     /locus_tag="ACOIAF_003803"
     CDS             1785..3056
                     /locus_tag="ACOIAF_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HGZ2670778.1"
                     /translation="MLKVLKGNIIFTKTSETFTVFENSYIILEKGKVKGIFKEIPKEY
                     KNLKVVDYTDKLIIPGMNDLHAHASQFKNLGMAMDKELLPWLETYTFPEESNYKDIEY
                     ATKMYKKFVKELWKSGTTRIAVFATVHKEASMKLMDIFKEVGLGAYVGKVNMNMNCPD
                     TLLENTQDSITDTEEIINKYSDTDSIVKPIITPRFIPSCTSELLKGLGDLCLKYNIPI
                     QSHLSENYDEIEWVRSLEPESKFYGDAYNRYNLFGQTPTLMAHCVHCSQDEIDLMREN
                     NVMAVHCPASNFNVGSGAMPIRKLIDNNIRLALGSDISGGHTLSIFKAMVSAIQLSKL
                     YWVNSGKKYNFLSLSEAFYIATKSGGSFFGKVGSFEEGYDFDALIIDDSNLNHDNYSI
                     LERLERFIYVGDDRNIIHRYVCGKLIEEPNI"
BASE COUNT         1205 a          417 c          391 g         1047 t
ORIGIN      
        1 attttaaagg ataaaatgct gtataaatta ttggggtatt ttacctatac ttgttgcaaa
       61 tttacatacc acgtagttaa tataaaacaa gatgttttta aaaataaact taatatagat
      121 attgaattta cataccacat agttaatata aaacaacttc tcatattcca gataattgtt
      181 attttacgaa atttacatac cacatagtta atataaaacc tgtagatttg cctgtttcga
      241 tattgctcat gtgtgaattt acataccaca tagttaatat aaaactctct tgtttcttct
      301 gttaatttta ttataccttc attatttaca taccacatag ttaatataaa acgcgttata
      361 ttgttgtata aaaccgatta aagttcaatt tacataccac atagttaata taaaacttgc
      421 aacatattgt tttatcgttg ctgggtcttt aaaaccattt acataccaca tagttaatat
      481 aaaactaagc gatattagag agcgcaaggc atagcggagc aaatttacat accacatagt
      541 taatataaaa cttctattaa gtgtaagcaa aacgcttgga atatgaaatt tacataccac
      601 atagttaata taaaacagat gaaacgcctg tgagaactga aaagttaaaa aaatttacat
      661 accacatagt taatataaaa cttatacaca aatacaactt gaagaagcgc taaagagatt
      721 tacataccac atagttaata taaaacctaa tgagtacata gataaacgta caggagaggt
      781 taatttacat accacatagt taatataaaa ctgaatttgc tcaaatagaa aaaaacaaag
      841 atttagaaat ttacatacca catagttaat ataaaaccaa atttgcctct ttcaaagtca
      901 taaattctat cagatttaca taccacatag ttaatataaa acttatgctt actgtagttc
      961 aaccattaga tatattagta tttacatacc acatagttaa tataaaacct taacatttta
     1021 cattccccct tattgtatat ttaagattta cataccacat agttaatata aaacataaat
     1081 aactttgttt ctttattttc tgtgtattta tttacatacc acatagttaa tataaaaccc
     1141 caaaataaac taagtatttt cagtacttac acacatacaa ctctctcaaa tttgcagtga
     1201 accatgagta gtgcaattga taacatttat cacacaccct caatccctta cattgcaact
     1261 gttaaaccct actttatcaa aaatatcgct cactgcaaaa tttatatatt tttattatat
     1321 cataaaattc aaactataaa attaaaatgg aataatttat caatttaaga acacctacat
     1381 atttgtaaat acttactata ttatctattc taataattct tcttctagct ggaactaata
     1441 taaaaatcat ctctgaaaga ttaggacata ctaatataaa aataactatg aatagatatt
     1501 ttcttgtttt aaacgatatg gataaaaaag cttctgaaaa tataatcaaa gttctcttta
     1561 aataacattc cattttatgt aagtaggcat tatatttttc ttaaatgtca cttaaatgtc
     1621 agttatatat aataatagta aacaactgta tttcactaat atttgctatt atctaatagt
     1681 atttatagtt tagatttgat ataatacatg tggtaaaatt ttgatttgta aaacaaaatt
     1741 ttacctacat atattaaaac aaatttaaaa ctaaggagac ctatatgtta aaagtattaa
     1801 aaggaaatat aatttttaca aaaacttcag aaactttcac agtatttgaa aatagttata
     1861 ttatactgga aaaaggtaaa gtaaagggaa tatttaaaga gatacctaag gaatacaaaa
     1921 acctaaaagt agtagattac actgataagc ttataatacc tggtatgaat gacctccatg
     1981 cccatgcctc tcaatttaaa aatcttggaa tggctatgga taaagagctt ttgccatggc
     2041 ttgaaactta tacatttcca gaagaatcaa attataaaga tatagaatat gcaacaaaaa
     2101 tgtataaaaa atttgtaaaa gaactttgga aatctggaac tacaagaata gcagtgtttg
     2161 caacagtaca taaagaagcc tctatgaaac ttatggatat attcaaagaa gttggtcttg
     2221 gagcatacgt tggtaaagta aatatgaata tgaattgccc tgatacttta ttagaaaaca
     2281 ctcaggattc tattactgat acagaagaaa ttataaataa gtatagtgat acagattcta
     2341 ttgtaaaacc aataataaca cctagattta taccaagctg tacaagtgaa cttttaaaag
     2401 gtttaggaga tttatgttta aaatataata ttcctattca atcacactta tctgaaaatt
     2461 atgatgagat tgaatgggtt agaagtctag aacctgaatc taaattctat ggagatgcat
     2521 ataatagata taatttattt ggtcaaactc ctactttaat ggctcattgt gttcattgtt
     2581 ctcaggatga aatagattta atgagagaaa ataatgttat ggcagttcac tgtccagctt
     2641 ctaactttaa tgttggtagt ggtgctatgc ctatcagaaa attaatcgat aataatatta
     2701 gattagcact tggatcagat ataagcggtg gacatacatt gtcaatattt aaagcaatgg
     2761 tatctgcaat acaattatct aaattatatt gggttaattc tggtaagaag tataatttct
     2821 tatctttatc tgaggctttt tatattgcaa ccaaaagtgg tggaagtttc tttggaaaag
     2881 ttggtagctt tgaagaaggt tatgattttg atgcattgat tattgatgat tcaaatttaa
     2941 atcatgacaa ttattctata cttgaaagac ttgaaagatt tatctatgtt ggtgatgata
     3001 gaaatatcat tcatagatat gtatgtggaa aactaattga agaacctaat atataattat
//
LOCUS       DBGJFF010000244         2948 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.249, whole genome shotgun sequence.
ACCESSION   DBGJFF010000244 DBGJFF010000000
VERSION     DBGJFF010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.249"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            425..2236
                     /locus_tag="ACOIAF_003804"
     CDS             425..2236
                     /locus_tag="ACOIAF_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HGZ2670779.1"
                     /translation="MKKKYDFLIYSAIWLVIIILFIFSLYHSIEHIKIISHTGTIRSE
                     TQKVVKQELNNERNDDLIKRLDNILIKLRTGNGENGFQRCDNKEFQQKLNQMDSMWES
                     MKKEIIKVRNGASGDKLYKLSEEYSVLSNQIVFISEKHSNAKLYSFATALFIYLIFST
                     ISLLIWEYYNKKRFKRIFYTDNLTKIKNQVAFENRAIEILHNASNKEYVLLNIDIDNF
                     KYINDTHGYEYGDKVLIIVAAALSKTFNIKETCARIGSDNFVILAKYRDSLLEDIREM
                     LTNAIISELDMNVTQTISYCIGAYLVEIDNLGYKSINSMMDKANIAHKVSKTRGISST
                     VWYNENLLKQLQMENSIYNYMYKAIENEEFHMYLQPKFQISSLNVVSAEALVRWFSPE
                     LGFLSPDEFIPLFEKSGFIIELDFYMLKKACSFVKKTFMKKNQYTYPIAVNFSRVTIY
                     QNSFYQRFLDTVKEYEIPFKYIEIEVTESAFNEISQPVISILEELKKLGFLISMDDFG
                     SGYSSLSLLCSLSINGLKLDKSLLKETFNREKVYSIIQCIIEMSHRIGMSVVCEGIET
                     KKDLEFLNTVKCDVGQGFYFSKPIEEKEFFNKYVTKK"
     gene            complement(2323..2904)
                     /locus_tag="ACOIAF_003805"
     CDS             complement(2323..2904)
                     /locus_tag="ACOIAF_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670780.1"
                     /translation="MLYLLKINQFEKSYDIGIFKNEQSIYKFIEKIPFVKKDNSNFNY
                     TNYYMEFEDIPEYYEVNFNNYLYIITRFSFIPDGTEIFFTWEEIHCWDERVATEKTFI
                     NGETLVDSYNFPNNEVKDYIQKRESLYIETKKYYEEKGLKIERHALGSEDGEYVELTN
                     DSILYLLDIEAVEIREQSKNIEEFLYKYKKRFK"
BASE COUNT         1116 a          303 c          416 g         1113 t
ORIGIN      
        1 ccaaaaaaaa ggaatatatt tacataatat gataaaataa attcaaagag ttaaattgta
       61 ggtaaatagt attttaatta tttattaaaa tatagattat gtatatgcca aatttaaaaa
      121 gagacataaa gacattatac aaatatatta ttggggtgat attttataag aaaagaagtg
      181 atgcagtaaa ttaattgagc aatttaaaaa tgatttaatg atagtgttga tgaatgttta
      241 aaaagaaatt ttagaaaaac gtatattgct gtttgtgttg gaggtgatga atttgtataa
      301 tttatagggt ataaatgatt aaactattta agataaatac atatcactgt aaaatattga
      361 taatcatttt ttataagaga ttgattattt gcaaattata tatattacaa atgaggtaac
      421 aataatgaag aaaaaatatg attttttaat ttattctgct atttggctag ttattattat
      481 tctttttata ttttctctat atcacagtat agagcatatt aaaattataa gtcacactgg
      541 tactattcgc agtgaaactc aaaaggttgt taagcaagaa ttgaataatg aaagaaatga
      601 tgacttgata aaacgactgg ataatattct gataaagcta agaacaggta atggagaaaa
      661 tggatttcaa agatgtgata ataaagaatt tcaacaaaaa ctaaatcaaa tggattctat
      721 gtgggaatct atgaagaaag aaattataaa agttagaaat ggtgcttcag gtgataaatt
      781 atataaattg agtgaagagt attctgtgtt gtctaatcaa attgttttta tatctgaaaa
      841 acactctaat gcaaaattgt attcttttgc tactgcacta tttatttatc ttatattctc
      901 aacaataagt cttttaattt gggaatatta taataaaaaa aggtttaaaa gaatatttta
      961 tactgataat ttaacaaaaa taaaaaatca ggttgcattt gaaaacagag cgattgaaat
     1021 tttgcacaat gcatctaaca aagagtatgt actgctaaat attgatattg acaattttaa
     1081 atatattaat gatacacatg gatatgaata tggagataaa gttctaataa tagttgcagc
     1141 tgcactttct aaaactttta atatcaaaga aacttgtgca agaattggtt cagataattt
     1201 tgtaatactt gcaaaatata gagattctct tttagaggat attcgggaaa tgctaactaa
     1261 tgctataatc tctgaattag atatgaatgt aactcaaact atatcttatt gtataggagc
     1321 gtacttagtt gaaattgata atttaggtta taaatctatc aattctatga tggataaggc
     1381 aaatattgca cacaaggtaa gtaaaacaag aggaatttcc tccacagtat ggtataatga
     1441 aaatctgtta aaacagttac aaatggaaaa tagtatttat aattatatgt ataaagcgat
     1501 agaaaatgaa gaatttcata tgtatttgca acctaaattt caaatatctt ctttaaatgt
     1561 tgtgagcgca gaagctcttg taagatggtt ttctccagaa cttggttttt tatcaccaga
     1621 tgaatttatt ccattgtttg aaaaaagtgg atttattatt gaattggact tttatatgtt
     1681 aaaaaaagcg tgttcatttg taaaaaaaac gtttatgaaa aaaaatcaat atacatatcc
     1741 gattgcagtt aatttttcaa gggttactat ttaccaaaat agtttttatc aaaggttttt
     1801 agatactgtt aaagaatatg aaattccttt taagtatata gagatagagg taactgaaag
     1861 tgcttttaat gaaatatctc aaccagttat ttcgatttta gaggagctta aaaagttagg
     1921 atttttaatt tcgatggatg attttggatc aggatactct tcattaagtc ttttatgttc
     1981 tctatctatt aatggattga agttagataa aagcttactt aaagaaacat ttaaccggga
     2041 gaaagtatat agtattattc agtgtattat tgaaatgtca catcgtatag gcatgtctgt
     2101 tgtatgtgaa ggtattgaaa ctaaaaaaga cttggaattt ttaaatacag ttaaatgtga
     2161 tgtaggacaa gggttttatt tttctaaacc gatagaagaa aaagaatttt ttaacaaata
     2221 tgtaactaaa aaataatata attattttta tcgtctatac attgagtcat tttatatctc
     2281 gttaaatgac tcaatgtatt gttatgtatt ttaaaatata aattatttaa aacgtttttt
     2341 gtacttatat agaaattctt ctatattttt tgattgttct cttatttcaa ctgcttctat
     2401 atctagtaaa taaagtatag aatcatttgt aagttcaaca tattcaccat cttctgaacc
     2461 gagtgcatgt ctctcaattt ttaatccttt ttcttcataa tatttttttg tttctatata
     2521 tagtgattct cgcttttgta tgtagtcttt cacttcatta tttggaaagt tatatgaatc
     2581 cactaatgtt tcgccattta taaaagtttt ctcggtagca actctttcat cccaacaatg
     2641 gatttcttcc caagtaaaaa atatctcagt tccatctgga atgaatgaaa acctggtaat
     2701 aatatataaa taattattga aatttacttc ataatattca ggaatatctt caaattccat
     2761 atagtaattt gtataattaa aattactatt atcttttttt acaaagggta ttttttcaat
     2821 aaatttatat attgattgtt catttttaaa aattcctata tcataacttt tttcaaattg
     2881 attaattttt aataaataca acattctttc tccttttatt caaaatataa tttttataaa
     2941 tttactaa
//
LOCUS       DBGJFF010000245         2929 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.250, whole genome shotgun sequence.
ACCESSION   DBGJFF010000245 DBGJFF010000000
VERSION     DBGJFF010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.250"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            439..1260
                     /locus_tag="ACOIAF_003806"
     CDS             439..1260
                     /locus_tag="ACOIAF_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HGZ2670781.1"
                     /translation="MKLKIVGSGGMSIIPSSFCKCKICEEARQKGGRYERLGPSLYID
                     DIKMLIDTPEDIAVACNRQKISEVKHISISHHDPDHVKGIRIVEKIGCDFITGENNPI
                     GFYALPEVIEDINRTNLDCLKYYGDVINCISINETSHVKINNFDIDLINNNPDRNITF
                     YVIKENEKKVIYACCNPKPFTHSDMYFDTDVLIISLVSDDGILNDGTKLKDTPFKDEI
                     FTLDEIVEIKNYYRIKKIIITHIDEMWGKSYGYYSELEKKLDNIYFAYDGMEIIV"
     gene            complement(1570..2349)
                     /locus_tag="ACOIAF_003807"
     CDS             complement(1570..2349)
                     /locus_tag="ACOIAF_003807"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HGZ2670782.1"
                     /translation="MSYKKIAIITGANSGFGKEFLKLLINEEEITEIWAVARNKARLN
                     QLVDEFGSKVKIFSKDLSKIEEVKEIGTFLSKENVCIKYLINNAGFAKFCSYNDLSID
                     ESINMIDLNISAVVALGLICIPYMQKGSHIMNISSQASFQPLPYQNIYSSTKAFIRNY
                     TRALNVELKSKGINAIAVCPGWMSTNLFKRGIVVAEKGTKNFPGIVSPDIVAKKALKD
                     AKKNKDISVYGINTKSSHLLAKLLPQKMMMKVWLMQQHIKE"
BASE COUNT         1093 a          372 c          355 g         1109 t
ORIGIN      
        1 aaattagttt ttttaacaaa acaataaaag agtaaagtac aaataattcc tttgtgctct
       61 actcttttta actttcttta gacaatgata taatgataaa tataaaaaat tatatattcc
      121 attctataga aatatgctca ccaataacat gtatgttgtt aatcaatata ttatatcaaa
      181 ttatatcaaa attacataaa ccttatagga caatcatatc ttattacaag aaaaatgtct
      241 tatccatatt gaataagtca ttcagttcat atacaaagtc aatttcattg agaaacattt
      301 aatatatgtt tataaaaatt tcatactatt ctttcatatt tgcatgtgat ataatttatt
      361 aactcataat tttgttaatc agttgtgctt gtatattatt tatataatct aatattttaa
      421 gaaggtggtg taataacttt gaaattaaaa attgttggta gtggcggtat gtcaattatt
      481 ccaagttcat tttgcaaatg caaaatatgt gaagaagcca gacaaaaagg cggaagatat
      541 gaacgattag ggccaagttt atatattgat gatataaaaa tgctgataga cacacctgaa
      601 gatattgcag tagcttgtaa cagacaaaaa atatcggaag taaaacatat ttcaatttct
      661 caccatgacc cagaccatgt taagggtatt agaattgtcg aaaaaatagg atgtgacttt
      721 atcacaggag aaaataatcc aattggcttt tatgcgcttc ctgaagtaat tgaagatata
      781 aaccgaacga atctcgattg tcttaaatat tatggtgatg ttataaattg catttctatt
      841 aatgaaactt cacatgtaaa gataaataat tttgatattg atttgattaa caataatcca
      901 gacaggaata taaccttcta tgttatcaaa gagaatgaaa aaaaagtcat atatgcctgc
      961 tgtaatccca agccatttac tcatagtgat atgtactttg atactgatgt tttaataata
     1021 agtctagttt ctgatgatgg tatattaaat gatggtacaa aacttaaaga cacaccattt
     1081 aaagatgaaa tatttacttt agatgagatt gttgaaatta aaaactatta tagaattaaa
     1141 aagattatca ttacacatat tgacgaaatg tggggaaaat catatggtta ttacagtgaa
     1201 ttagaaaaga aacttgataa catttatttt gcatatgatg gtatggaaat tatagtctaa
     1261 atcattaagt aataagcata atgcaaaata agaaaacttc aatttaacag tatagttaaa
     1321 ctatgtaata tcaacatatc tcagttttta caaaatatca tagtatttat ctttataaaa
     1381 tttcaagtcc tacatatttt aatacttcaa tatctaaata aagtaaaatc aaaaatatat
     1441 tttatataca ttatagacgg cttatcggca ctagataagc cgttttgtca caattattat
     1501 ttgctcaaca aaacttctca taatatataa cagattgaat ctccaactga tacttaatta
     1561 ccgaaactct tattccttaa tatgttgttg cattaaccaa accttcatca tcattttttg
     1621 tggcaatagt tttgctaata aatgactgga ttttgtattt ataccataca cagaaatatc
     1681 cttatttttc tttgcatcct tcaatgcttt tttggcaaca atgtctggag acactatacc
     1741 tgggaaattt tttgtccctt tttcagctac aactatacct ctcttaaata gatttgtact
     1801 catccaacct ggacatacag caattgcatt tatcccttta ctcttaagtt caacatttaa
     1861 tgctcttgta taatttctta taaacgcttt agttgaacta taaatatttt gatatggcaa
     1921 tggctggaat gatgcttgtg aagaaatatt catgatatgg cttccctttt gcatatatgg
     1981 aatacatata agtcccaatg ctacaacagc actaatattt aaatcaatca tattaataga
     2041 ttcatctata cttaaatcat tgtatgaaca gaattttgca aaaccagcat tgtttataag
     2101 gtattttata catacatttt ctttacttaa aaatgtacca atttctttta cttcttctat
     2161 ttttgataaa tcttttgaaa aaatttttac cttacttcca aattcatcta ctaattgatt
     2221 taatctcgcc ttgtttcttg caactgccca tatttctgtt atttcttctt catttattaa
     2281 taactttaga aactctttac caaaaccact attagcaccc gttataattg caatcttctt
     2341 ataactcata attattctcc tttaccataa gtttatacgc tcaaactgta aacttatttt
     2401 ttacaaataa aacaccctct attcagatgt tatattcata ttatttgaga tattatattt
     2461 atatcatttt caataattgt tatatagcat aaaaaatagt gttaatatta tagaatcggt
     2521 attgattata tttaaaaagt agtgttaatt atattataga atcagtattt attatattat
     2581 aaaaagtagt gttaatatta tagaatcgat attgattata tttaaaaagt agtgttaatt
     2641 atattgtaga atcagtattg attatattgt aaaaagtagt attaataata ttgtagaatc
     2701 aatattgatt atatttaaaa aagtagtgtt aattatatta tggaatcaat attgattata
     2761 tttaaaaaag tagtgttgat tatattataa aaagtagtat taataatatt gtagaatcag
     2821 tattgattat atttaaaaaa gtagtgttaa ttatattatg gaatcagtat tgattatatt
     2881 taaaaaagta gtgttaatta tattatggaa tcagtattga ttatattta
//
LOCUS       DBGJFF010000246         2923 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.251, whole genome shotgun sequence.
ACCESSION   DBGJFF010000246 DBGJFF010000000
VERSION     DBGJFF010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.251"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            129..497
                     /locus_tag="ACOIAF_003808"
     CDS             129..497
                     /locus_tag="ACOIAF_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HGZ2670783.1"
                     /translation="MENLVKIFKALSDETRLNILILVSKRNICQKGISKYLGISDSAV
                     SQHIKILKDVGIITGYKEGYYVLYHINKESFNICVDFINSMLSNSSESFIDVFDVNTI
                     HLGCSKECKSIKKCCKRREK"
     gene            500..871
                     /locus_tag="ACOIAF_003809"
     CDS             500..871
                     /locus_tag="ACOIAF_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HGZ2670784.1"
                     /translation="MKVCIPVEENKGLDSKPYGHFGSAPIFVVCDLESGEVKSLDNGD
                     LDHEHGKCQPLKALSGTAVDAVVVGGIGQGAIIKLNGMGIKVYRAEGDTISANLDLLK
                     NGKLVEFPSDHTCSHDGCGHH"
     gene            complement(1015..1470)
                     /locus_tag="ACOIAF_003810"
     CDS             complement(1015..1470)
                     /locus_tag="ACOIAF_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670785.1"
                     /translation="MNINTVQGDSIEVLLRQLGATRISKVSSTLYFIKFDLGDGWEIS
                     YTYNINAKDQYFLQRIEPYPIGRGLFNDEYEIVSFISKDLKKFLNAKNSSNFKTFVEV
                     TRKVNSIIDNVEELFLNYNVDGDDLKTLNKELNDILNDIDYIEKHTKKI"
     gene            complement(1509..2774)
                     /locus_tag="ACOIAF_003811"
     CDS             complement(1509..2774)
                     /locus_tag="ACOIAF_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HGZ2670786.1"
                     /translation="MEFQQIIAICIFLIVMAAIITEKVNRSVAAVGGALLMIIFNILT
                     LDEGLSHIDFNTIGVLVGMMLFVAVVKNSGLFEYIAIWTAKKAKGDPWKIMICFAIIT
                     AILSAVLDNVTTVLLIGPMTIVITQILGLNPVPFLITQILASNIGGTATLIGDPPNIM
                     IGSAANLSFMDFVINLGPAVIVILAITIICFRFIYGKELVVNERAKNAILKLDEKKSV
                     KDKPLLIKSLILIAFILFGFMFHSTIHIDSSVVALTGASIMLLIGKQDVDEIMAGIEW
                     STILFFMGLFVVVGGLVEVGIINKLAQALIGLTEGHLVFTMLLILWLSAIVSSFLDNI
                     PFVATLIPLILTMQAEGIDVMPLWWATSLGACLGGNGTLIGASANVVLAGIGNKHGHP
                     ISFKEYFKIGFPLMIISIIISTVYLIIKF"
BASE COUNT         1079 a          405 c          407 g         1032 t
ORIGIN      
        1 tcaaagttat attagtaaaa attggaatta tttataatat actccaaaat ttaaaaataa
       61 tggttgaaaa atctaattaa gagtactata atttaattaa gtaattactt aatcaaaacg
      121 aggtacttat ggaaaacttg gttaaaatat ttaaagcatt gagtgatgaa actagactta
      181 acatacttat attagtatcc aagagaaata tatgtcaaaa ggggatttct aaatatctag
      241 ggatttcaga ttctgctgtt tcacaacata taaaaatatt aaaagatgtt gggataataa
      301 caggatataa agaaggatac tatgttttat atcatattaa taaagaatca tttaatatat
      361 gtgtagattt tataaattct atgttaagta attctagtga aagctttata gatgtatttg
      421 atgtaaatac aatacatcta ggatgcagta aagaatgtaa atctataaaa aaatgttgta
      481 agagaaggga gaaataataa tgaaagtatg tataccagta gaggagaata aaggattgga
      541 tagtaaacca tatggacatt ttggttctgc tccaattttt gttgtgtgtg atttagaaag
      601 tggagaagtt aaatctttag ataatggaga tttagatcat gaacatggaa aatgtcaacc
      661 attaaaagca ttgtcaggga cagctgtaga tgcagtagtt gttggaggaa taggacaagg
      721 agctataatt aaattaaatg gaatgggaat aaaagtttac agagcagaag gtgatactat
      781 aagtgctaat ttagacttac ttaaaaatgg aaaattagta gagtttccat cagaccatac
      841 ttgctcacat gacggatgtg gacatcatta aaaaaaacag ctgatgaaat atatacttca
      901 tcagctattt tttcttttaa taaatacgat tttattcttt taaaaccaaa atttagattt
      961 ttatgttttt tgtagtatcc ttaataatat ttcaagttta caagtttact acttttagat
     1021 tttttttgta tgtttttcta tatagtcaat atcatttaat atgtcattga gttctttatt
     1081 aagtgttttt aaatcatcac catctacatt gtagtttaaa aatagttcct ctacattatc
     1141 aatgatagaa ttgacttttc tagtaacttc aacaaatgtt ttaaagttac tactattttt
     1201 agcatttaga aattttttta aatcctttga tataaatgat actatttcat attcatcatt
     1261 aaatagacct ctaccaatag gatatggttc aattctctgt aaaaaatatt ggtctttagc
     1321 attaatatta taagtataag atatttccca accatctcct aaatcaaact tgataaaata
     1381 taatgtacta cttacttttg atattcttgt ggcaccaagt tgtcttaaaa gaacttcaat
     1441 cgaatcacct tgaacagtgt taatattcat atatacacat cccctttagt attattatac
     1501 tatatttttt aaaattttat aattaaataa acagtagata taataataga tataatcata
     1561 agtggaaaac ctattttgaa atactcctta aaagagatag gatgaccgtg tttattacct
     1621 atacctgcta gtacaacatt agcagatgct cctattagtg ttccatttcc tccaagacaa
     1681 gctccaagag atgttgccca ccatagtggc attacatcaa taccttcagc ttgcatagtc
     1741 aaaataagag gaattagtgt tgcaacaaat ggaatattat ctaagaaaga agagactata
     1801 gctgataacc ataatatcaa caacattgta aatacaagat gtccttctgt aagtccaatc
     1861 aatgcctgag ccaatttgtt tattatccca acttctacta atccaccaac tactacaaat
     1921 aatcccataa agaataaaat tgtagaccat tcaatacctg ccataatttc atctacatct
     1981 tgttttccta ttaaaagcat tatagatgct cctgtcagtg ctacaacaga agaatctatg
     2041 tggattgtac tgtgaaacat aaaaccaaaa agtatgaatg ctattaatat aaggcttttt
     2101 ataagtagag gtttatcttt aacagatttt ttttcatcta gttttaatat tgcattttta
     2161 gctctttcat tcacaacaag ttcttttcca taaataaatc taaagcatat tatagttata
     2221 gcaagtatta ctataacagc aggacctaaa tttattacaa aatccataaa actaagattg
     2281 gcagcactac caatcataat atttggaggg tctcctataa gagtagctgt tcctcctata
     2341 tttgaagcta aaatctgtgt tattaaaaat ggaactggat taagtcctaa tatttgtgta
     2401 ataactatag tcattggtcc aataagtaag acggttgtaa cattatctaa tacagctgat
     2461 aaaatagctg ttataatagc aaagcaaatc attatcttcc atgggtcacc tttagctttc
     2521 tttgcagtcc agatagctat atattcaaat aaccccgagt ttttgacaac agctacaaat
     2581 agcatcatac caactaatac accaatagta ttgaaatcta tatggcttaa tccttcatca
     2641 agtgtaagta tattgaatat tatcattaat agtgcaccac caacagctgc tacggatctg
     2701 tttacctttt ctgtaataat agctgccata actattaaaa atatacatat agctattatt
     2761 tgttgaaatt ccataatata agtacctcca tttgtattat tttgtgctta atgctagtac
     2821 tatatatagt attacttttc tattgttttt tcaatggatg tatgaaagta tattatttaa
     2881 caaagttttt gaaaaattga cagaatttaa agaaaatttt tgt
//
LOCUS       DBGJFF010000247         2922 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.252, whole genome shotgun sequence.
ACCESSION   DBGJFF010000247 DBGJFF010000000
VERSION     DBGJFF010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.252"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            310..1581
                     /gene="hflX"
                     /locus_tag="ACOIAF_003812"
     CDS             310..1581
                     /gene="hflX"
                     /locus_tag="ACOIAF_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HGZ2670787.1"
                     /translation="MAKKGITVGLNINNKSEDFNELMIELENLCSACDIDVVGSITQN
                     AKQVNRAFYIGTGKVEELLNLIKKENIEIVIFYNELSTSQLKNLEEKLNCEIIDRTAL
                     ILDIFAQRAKTREAKLQVEVASLKYMLPRLIGSNENLGRQSGGVGTKNKGSGEKKLEL
                     DRRRIEEKITSLNKELDDLKFQRETQRSMRRKSNLPNVALVGYTNAGKSSIMNKLVDI
                     FKNSEEKKVFEKNMLFATLETSVRNIVLANNKEFLLSDTVGFVSNLPHDLVKAFRSTL
                     EEACEADVLLHVIDISNPSYKSHIKVTEDTLKQIGADGIPMIHVYNKIDLIDVEVLDK
                     ILDSIDKEGLFVSVKKDINIDKMIKCICDSIFKDYVRCKFLIPYDKGHVASYFNENTS
                     VINTEYREDGTLLDVECSNIEYNKYKKYVLE"
     gene            1823..2320
                     /locus_tag="ACOIAF_003813"
     CDS             1823..2320
                     /locus_tag="ACOIAF_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HGZ2670788.1"
                     /translation="MDAETLMKKFMEDDDSRKQAIFASIFVMQNKLQTTCDKLDPELT
                     MKQWLLLAIADSVDETLTLTKLGELMGCSRQNVKKLAIALEKKGFIQIEQNEKDSRAI
                     CLVTKDRVEEYSKKVRDMQEKVLKLLFEDFTEKEVEQLFFGIMKLSTGIENVKNYVEN
                     NDVGE"
     gene            2369..2893
                     /locus_tag="ACOIAF_003814"
     CDS             2369..2893
                     /locus_tag="ACOIAF_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HGZ2670789.1"
                     /translation="MKSVVVIYKSKYGSTEKYAKWLSELLSCDIFETKNINIEKVMKY
                     DTIVLGGGLYACNIAGISFFKKNYKELQKKNLVVFGVGAAPYDDEVIKKLREVNFKDE
                     LNKVPCFYCRGAWDETKMSFIDYTLCSFFKKSIAKKERQGLKPVEAPLLSGEAFDWCN
                     KENLTPILDYIREL"
BASE COUNT         1211 a          261 c          517 g          933 t
ORIGIN      
        1 taatgtattt ttaatatata tttagaactt taatccttct tatgatataa gtagcttgtt
       61 ttgtattact tagatttgta aggagttttt ttattgaaaa aactgcataa tatatttggg
      121 aggaggtgta agaaagtgat tttttatata agattgagaa aataatgcac gattatggtt
      181 attataatag taaataagaa gtgtcattta aggataagaa aaaagcaaaa ttcttgatga
      241 atttaattat agagccataa tctcgtgcta tagattaaat aaaaaatagc agataaggag
      301 aatatggata tggcgaaaaa aggaataaca gtaggattaa acataaataa taaatctgag
      361 gattttaatg aactaatgat tgaacttgaa aacttatgtt cagcatgtga tattgatgtt
      421 gtagggagta taactcaaaa tgcaaagcag gttaatagag cattttacat tgggacaggt
      481 aaagttgagg aactattaaa cttaataaag aaagaaaaca tagagatagt tatattttac
      541 aatgaacttt ctacatcaca actaaaaaat cttgaagaaa agcttaattg tgaaattata
      601 gatagaacgg cattgatact tgatatattt gcacaaaggg caaaaactag agaagctaaa
      661 cttcaagtag aagtagcaag tttaaaatat atgttaccaa gacttatagg ttcaaatgaa
      721 aatttaggac gtcaaagtgg tggagttggt actaaaaata aaggttctgg tgagaaaaaa
      781 ttagaacttg accgtagaag aatagaagaa aagataacat ctttaaataa ggagttagat
      841 gatttaaaat tccaaagaga aactcaaaga agtatgagaa gaaaatcaaa tcttccaaat
      901 gtagctctag ttgggtatac aaatgctggt aaatcttcaa taatgaataa actagtagat
      961 atatttaaaa actctgaaga aaagaaagtg tttgagaaaa acatgttatt tgctacactt
     1021 gaaacttctg tcagaaatat agttcttgca aataacaaag aatttttatt atctgatact
     1081 gttggttttg taagtaatct accacatgac ttagtaaaag catttaggtc aacattagaa
     1141 gaagcatgtg aggcagatgt actgttacat gttattgata tttcaaatcc aagttataaa
     1201 agtcatatta aagtgacaga agatacttta aaacaaatag gtgcagatgg tatacctatg
     1261 atacatgtgt ataataaaat tgatttaata gatgtagaag tattagataa aattttagat
     1321 agtatcgata aagaaggcct atttgtttca gttaaaaaag atataaatat agataaaatg
     1381 ataaaatgta tttgtgatag catatttaaa gattatgtaa gatgtaagtt cttaattcca
     1441 tatgacaaag gtcatgtagc ttcttatttt aatgagaata caagcgttat aaatacagaa
     1501 tacagagaag atggaactct tttagatgta gaatgtagca atatagaata caataaatat
     1561 aaaaagtatg ttttagagta agttttgaaa aaattattat agcttaaaat acatttaatt
     1621 tataatttta taataaatta taaaattatt gtaaaatatg attttatgaa acggtatagc
     1681 cataaagcta tgccgttttg ctatttataa taaaaataaa tttattgaca caatagtaac
     1741 taattactat aatataaagt aactagttac tatttaaagt tatcaaaaaa ttaaaatcta
     1801 agataaagag aagtgtataa taatggatgc agaaacttta atgaaaaaat ttatggaaga
     1861 tgatgatagt agaaaacaag ctatatttgc aagtatattt gtaatgcaaa ataaacttca
     1921 aactacatgt gataaattag acccagaatt aacaatgaaa caatggttac ttttagctat
     1981 tgcggactct gtagatgaaa ctcttacatt aaccaaactt ggagaattaa tggggtgctc
     2041 aagacaaaat gtaaaaaaac tagctattgc tttagaaaag aaaggtttta tacaaattga
     2101 gcaaaatgaa aaagattcaa gagctatctg tcttgtgacg aaagatagag tggaagagta
     2161 ttctaaaaaa gtaagagata tgcaggaaaa agtattaaag cttttatttg aagattttac
     2221 agagaaagaa gtggaacagt tattttttgg aattatgaaa ttaagtacag gaattgaaaa
     2281 cgtgaaaaat tatgtagaaa ataatgacgt tggagaataa gcaagtgtca aataaacttt
     2341 gtatgacatc agaaagaggg taatttatat gaaaagtgta gtagtgattt ataaaagtaa
     2401 gtatggttca acagaaaaat atgctaaatg gctttcagaa ttattgtctt gtgatatttt
     2461 tgaaactaag aatattaata ttgaaaaggt tatgaagtat gatacgattg ttttaggagg
     2521 aggtctttat gcttgtaata tagcaggaat ttctttcttt aaaaaaaatt ataaagaatt
     2581 acagaaaaaa aatctagtag tatttggcgt aggagctgct ccatatgatg atgaagtaat
     2641 aaagaagtta agagaagtaa actttaagga tgaattaaac aaagttcctt gcttttattg
     2701 tcgtggggca tgggatgaga caaaaatgtc ttttatagat tatactcttt gtagtttttt
     2761 taaaaaatct atagctaaaa aagaacgtca aggtcttaaa ccggtagaag cacctttatt
     2821 gtctggagaa gcctttgatt ggtgtaataa agaaaattta actccaatac ttgattatat
     2881 aagagaactt taagaggtat atatttgtaa ttatttatta tt
//
LOCUS       DBGJFF010000248         2730 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.253, whole genome shotgun sequence.
ACCESSION   DBGJFF010000248 DBGJFF010000000
VERSION     DBGJFF010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.253"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            246..1664
                     /locus_tag="ACOIAF_003815"
     CDS             246..1664
                     /locus_tag="ACOIAF_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HGZ2670790.1"
                     /translation="MTELMIISGLVLLICITSSKVLYKFGVPILLIFILLGMLFGSDG
                     MVGIYFDNYELTKQLCSIGLIFIMFFGGFGTNWNMAKPVAIPSILMSSLGVIITAGVT
                     GMFCHMVIGTSLLEGLLIGAIAASTDAASVFAVLRSQKLNLKGSLASLLEVESGSNDP
                     IAYMLTLILLTLMNNSGVELIAPMVIKQIAFGLAIGFILAKLSVYILRHSNFEIEGFY
                     TIFITAIAILSYAFSEYLGGNGYLSVYISGIIIGNSRIPLKKSLVHFFDGISWIMQIM
                     LFFMLGLLSFPSKLPNVFGIAMAISIFMIVIARPLATFIVLAKFKFSNKEKLFISWVG
                     LRGAASVVFAIYAVTQGVAIENDIFHIIFFIALLSVGIQGSLIPTVARKLDLVDDNTP
                     VLKTFNDYDGEISSKLIEVNVDEGNKWANKSIIDSNIPDDILIVMIKRKEQVLIPKGS
                     TIIKTGDILVLSGDRIEELIQL"
     gene            1740..2552
                     /locus_tag="ACOIAF_003816"
     CDS             1740..2552
                     /locus_tag="ACOIAF_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HGZ2670791.1"
                     /translation="MFSIGMFSKINKITTKTLRYYEDIGLLKPEYVDEFTKYRYYTTE
                     QLPKLHQIITLKQMGLSLAEIKKAVENPEEVENILMNKEKEMLDVIKSEEYKIIKLRN
                     YLMNLKGEVNMNDVIIKSLPKVKVASMRKVLKGYDELFNLFPNVMAEEMMKTGCTCAV
                     PDYCFNIYHDGEYRETDMDVEMCQAINDLKEDTDILKFKELDAVENAVCILHNGPYST
                     LGKTYADAFKWIEDNKYKVVGNPRESYIDGIWNKESEEDWLTEVQIPVKRML"
BASE COUNT         1024 a          278 c          482 g          946 t
ORIGIN      
        1 attaaacaat caactaaaaa atgaaatatt aataaaaaaa aattcaaatt ataaatatta
       61 aatgaatata attaaaacaa atatataaat ttaagtataa cactcacatt ttaatgtatt
      121 gtgaaatttt tgttgaactt tatgtaccat aacttataaa atttatgtca tgttatattt
      181 gaataagtaa taaaattgta gatgtaaatt aatctacaaa atttatatga aatggagttg
      241 atgctatgac agaattaatg attataagtg ggttagtact attgatttgt ataacttcta
      301 gcaaggtatt gtataaattt ggagtaccaa tattattgat ttttatctta ctagggatgt
      361 tatttgggtc tgatgggatg gtaggaatct attttgataa ctatgaactg acaaaacaat
      421 tatgttctat tggactaata tttataatgt tttttggagg gtttggaaca aactggaata
      481 tggctaaacc tgttgctata ccttctatat taatgtcttc actgggtgta ataattacgg
      541 ctggcgttac aggtatgttt tgccacatgg taataggaac tagtttactt gaaggtttat
      601 taattggggc aattgcagct tcaacagatg ctgcatctgt gtttgctgtg ttgcgttctc
      661 aaaaactaaa cttaaagggt tcacttgcat cattattaga agttgaaagt ggaagtaatg
      721 acccaatagc gtatatgttg acactaattc ttttgacatt gatgaataat tctggggtag
      781 aacttattgc accaatggta ataaaacaaa tagcttttgg gttagctata ggatttatac
      841 tagcaaaatt atctgtttat attttaagac attcaaactt cgagatagaa ggattttata
      901 ctatatttat aacagctatt gcaatacttt cttatgcatt tagcgagtat cttggaggaa
      961 atggatattt aagtgtttac atttcaggta ttattatagg aaatagtagg atacctctta
     1021 aaaaaagttt ggttcatttc tttgatggaa tttcatggat tatgcaaata atgttattct
     1081 ttatgttagg attgttatct ttcccttcta agttaccaaa tgtatttggt atagcaatgg
     1141 ccatatctat attcatgata gttattgcaa gaccattagc aacatttata gttctagcaa
     1201 aatttaaatt ctcaaataaa gaaaaattat tcatatcgtg ggtaggtctt agaggtgcgg
     1261 cttcagtagt ttttgcaatt tatgctgtta ctcaaggagt agccatagag aatgacatat
     1321 tccatataat attttttata gcgttgcttt cagtaggaat acaaggttca ttgataccaa
     1381 ctgtagccag aaagttggat ttagttgatg ataatacacc tgtattaaaa acatttaatg
     1441 attatgatgg agaaattagc tctaaactta tcgaagtaaa tgttgatgag ggtaataaat
     1501 gggctaataa gagtataata gattctaata taccagacga tatattaata gttatgataa
     1561 aaagaaagga acaggtttta atacctaaag gttcaaccat tataaaaaca ggggatattt
     1621 tggtattgag tggagataga attgaagaat taatacaatt ataatttatt ttgtattgac
     1681 tttaacctaa ggggaaagtt taaacttaaa ttaatcaaat ctaatgcgca ggtgatgaga
     1741 tgttttctat aggaatgttt tctaaaataa ataaaataac tacaaagaca ttaaggtatt
     1801 atgaagatat agggttgtta aaaccagaat atgtagatga atttacaaaa tataggtatt
     1861 atacaacaga gcaattacct aaattacatc agataataac tttaaaacaa atgggtttat
     1921 cattagctga gataaaaaaa gctgtagaaa atcctgaaga agttgaaaat attttaatga
     1981 ataaagaaaa agaaatgttg gatgtaatta aaagtgaaga atataaaata attaaactac
     2041 gtaattacct tatgaatttg aaaggagaag tcaatatgaa tgatgtaatt attaagtctt
     2101 taccaaaagt taaagttgct tctatgagaa aagtgttaaa ggggtatgat gaacttttta
     2161 atctatttcc aaatgtaatg gctgaagaaa tgatgaaaac agggtgtaca tgtgcagttc
     2221 ctgattattg ttttaatata tatcatgatg gtgaatatag agagactgat atggatgttg
     2281 aaatgtgtca agctataaat gatttaaagg aagatacgga tatattaaaa tttaaagaat
     2341 tagatgcagt tgaaaatgca gtttgtatat tacacaatgg tccatattca acattaggaa
     2401 aaacttacgc agatgcattt aaatggatag aggacaataa atataaagtg gttggaaatc
     2461 ctagagaatc ttatatagat ggtatatgga ataaagagag tgaagaagac tggttgacag
     2521 aagttcagat accagtgaaa cgcatgttat aatatgtcta caaagtataa ataagaataa
     2581 aaaaggtggt gtctcaaagt aaacatttta gtttatgaga cactcttttt catatgaaat
     2641 caagtatatt tttataatta cgattatgaa aaaaggctta tctatatttt tagacagcct
     2701 cttatttttt tattataata gttttctaaa
//
LOCUS       DBGJFF010000249         2728 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.254, whole genome shotgun sequence.
ACCESSION   DBGJFF010000249 DBGJFF010000000
VERSION     DBGJFF010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2728
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.254"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            <1..1160
                     /locus_tag="ACOIAF_003817"
     CDS             <1..1160
                     /locus_tag="ACOIAF_003817"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HGZ2670792.1"
                     /translation="TEYINELFNIISIEDIESYIISNIDLKNLKIIIKKKKDICDYLN
                     KISNYKKVKSTIEKLETRGKMPKWYLELINKVKNILENMLYNRNLSDEQIFYLNLFLD
                     IQNLFLGIYELKSESVIFENTLDDNKIIAKMDTYIIIDYINASFEYLGYVLNGSALNS
                     IDYGVPQNRERFVLIGAKKDFLKNKKIETPKPIVGENYVTVRDAIGDLAEYEASKGSM
                     DYCFEKNSNNIEKDFYRKIILDSNKIYNHVCTDTRNIALKRFEYINQGNNFHSLPDEL
                     KGTYADPERTQNTIYKRLVYDKPSDTVVNVRKSMWIHPIKNRAVSAREAARLQSFPDS
                     YKFLGTKDSVYQQIGNAVPPLLGRVVAEKILNLFNDEPNEYLRDVFEALDG"
     gene            complement(2160..>2728)
                     /locus_tag="ACOIAF_003818"
     CDS             complement(2160..>2728)
                     /locus_tag="ACOIAF_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HGZ2670793.1"
                     /translation="KLVKILINIICIIALTFSSLSIYIKLSEYKKADEVYTELRENTI
                     NNSKHQELYDKNNDYRFWLKINNVNINYPVVQGYNNDFYLTHDFYKNYLPLGSIFMDY
                     RNNFENDKSLIVYGHYMKNKTMFGQLENYTDEVFFKENNLVEINYKVQTYTYEIFSVY
                     TADLINRDYLSIHFNNNDEFKYSLNYIT"
BASE COUNT         1052 a          311 c          363 g         1002 t
ORIGIN      
        1 gaactgagta tataaatgaa ttatttaata ttattagtat agaagatatt gaatcatata
       61 ttatatcaaa tatagactta aaaaatttga aaataattat taagaaaaaa aaggatatat
      121 gtgattattt aaataaaata tctaattata agaaagtaaa atctaccata gaaaaattgg
      181 aaacaagggg aaaaatgcca aaatggtatt tagaattaat aaataaagta aaaaatatat
      241 tagaaaatat gttatataat agaaatttat cagatgaaca aatattttat ttgaacttat
      301 tcttggatat tcaaaattta tttttaggaa tatatgagtt aaaatctgaa tctgtaattt
      361 tcgaaaatac acttgatgat aataaaatta tagctaaaat ggatacttac attataatag
      421 attatataaa tgcatccttt gagtatctag gatatgtatt aaatggaagt gcactaaatt
      481 caattgatta tggagtacct caaaatagag aaagatttgt attgatagga gcaaaaaaag
      541 acttcttaaa aaataaaaag atagaaactc ctaagcctat agttggagaa aattatgtta
      601 cagtaagaga tgcaataggc gatttagctg aatatgaggc ttctaaaggt agtatggatt
      661 attgttttga aaaaaatagt aataatattg aaaaagattt ttatagaaag ataatattgg
      721 atagcaataa aatatacaat catgtatgta cagatacaag aaatatcgca ctaaaaagat
      781 ttgaatatat aaatcaagga aataattttc attctttgcc agatgagtta aaaggaactt
      841 atgctgatcc agaaagaact caaaatacta tttataaaag actagtatat gataagccat
      901 cagatactgt tgtaaatgta agaaaatcta tgtggataca tcctattaaa aacagagctg
      961 ttagcgcaag agaggcggct agattgcagt cattccctga tagttataag tttttaggta
     1021 caaaagattc tgtatatcaa cagataggaa atgcagtacc acctttatta ggtcgagtag
     1081 tagcagaaaa gatattaaat ttatttaatg atgaaccaaa tgagtatctt agagatgtgt
     1141 ttgaagcact agatggatag ttcaaataaa atagtgggta ttattcttgt gattcttatc
     1201 caacattaat ccacgaaata aggactgtcg aatgagtaat tttaaattgc tgtgcgacag
     1261 ttcttttttt acttgctttt cattaattta tatttatgca aaatttgagc ttgaaaacgg
     1321 tcataagaaa taaaggtata aaaatacaat tttaaaatag aataatttac aaagtgtaag
     1381 attaaaaaat atttttgaga ggatgaatat acatactaca tttattaatg attatcttat
     1441 ttgtgaaact tagaaatact ataggctaac actacaggta taaactctga aagaatattt
     1501 tgattgcctt tgattagaat tcttttaaag ttcatactat gttttatttg ggtgaaagct
     1561 ccttatgcct gaatacttct attcattcta agttcacatc cttgtgtacc agtgattctt
     1621 tctaaattat cttaatgcca aaatcttgtt cgcatttcaa aataaagtca ggtagctact
     1681 tcaacatttt agttatattt cttcaatagt ttgtctccaa acaaaggtac atctattaga
     1741 aacagattta attttattta tatcaataaa taatatatac tttgaaactt cattaatatc
     1801 accaagataa tttataagtt aagctattat atactatctt caccaactac atcctccata
     1861 gttttacact ttcttaattc agcttggtaa tctaaatctg taataattat ttctctttga
     1921 tttttcattt ctttgcctac aaaagtttaa tataaatatt attccatact taccaatttt
     1981 acgttcaaaa aagactgtag ataagtctgc atttacacaa acctacttat aggtctcttt
     2041 tttgaaatta atttagcatg aactatagta agagcatctt taaattcata gttacaagta
     2101 tacaaagtta taattttatc tgaaatatct acctttatat cagatttgtg tactgatttt
     2161 taggttatat aattcaaaga atatttaaac tcatcattat tattaaagtg tatacttaaa
     2221 taatctctgt taattaagtc agcagtatat actgaaaaaa tttcataagt ataagtttgt
     2281 actttataat ttatttctac taagttattt tctttgaaaa atacttcatc agtatagttt
     2341 tctagttgac caaacatagt tttatttttc atataatgac catagactat taaactctta
     2401 tcattttcaa aattatttct ataatccatg aaaatactac ctaaaggaag ataattttta
     2461 taaaaatcat gagttaggta gaaatcattg ttatatcctt gtactacagg atagtttata
     2521 tttacgttgt ttattttaag ccagaatcta taatcattat ttttatcata aagttcttga
     2581 tgtttgctat tattgatagt gttttctctg agttccgtat atacttcatc agctttttta
     2641 tattcagata attttatgta aatcgaaagg ctagagaaag ttagcgcaat aatgcaaata
     2701 atatttatta atatttttac tagttttt
//
LOCUS       DBGJFF010000250         2666 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.255, whole genome shotgun sequence.
ACCESSION   DBGJFF010000250 DBGJFF010000000
VERSION     DBGJFF010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.255"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            665..934
                     /locus_tag="ACOIAF_003819"
     CDS             665..934
                     /locus_tag="ACOIAF_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HGZ2670794.1"
                     /translation="MFESRCGVCCDSCTKKEQVNCTGCPTMEKPFWGGECKVKTCCES
                     KELNHCGECDTFPCDMLLNKGKDQGFDPMVNIEQCRKWLEETVSN"
     gene            complement(1152..1397)
                     /gene="cdd1"
                     /locus_tag="ACOIAF_003820"
     CDS             complement(1152..1397)
                     /gene="cdd1"
                     /locus_tag="ACOIAF_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein Cdd1"
                     /protein_id="HGZ2670795.1"
                     /translation="MGELQKIPGVGKATEKSLIMLGYTTIKSLKDANPAQMYEKECLM
                     RGQHIDRCQLYVYRCAVYFASTENPEPEKLKWWYWKD"
     gene            1823..2521
                     /gene="tcdC"
                     /locus_tag="ACOIAF_003821"
     CDS             1823..2521
                     /gene="tcdC"
                     /locus_tag="ACOIAF_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin anti-sigma factor TcdC"
                     /protein_id="HGZ2670796.1"
                     /translation="MFSKKNEGNEFSNEGKGSSKKIIKFFKSTKGIALLAFILGVFFG
                     NISSPSCSEDHEEVISNQTSVIDSQKTEIETLNSKLSDAEPWFKMKDDEKKAIEAENQ
                     RKAEEAKKAEEAKKAEEQRKKEEEEKKGYDTGITYDQLARTPDDYKYKKVKFEGKVIQ
                     VIEDGDEVQIRLAVSGNYDKVVLCSYKKSITPSRVLEDDYITIRGISAGTITYESTMG
                     GNITIPGIAVEKIN"
BASE COUNT          978 a          285 c          398 g         1005 t
ORIGIN      
        1 ttttatatta tataatgaaa cttaattaaa tataggtaaa aaatcattca atatattaaa
       61 taaatattta actttaataa tctatattta attatagcta taacatagaa ttatcaaata
      121 tattgaatga atatttgaat cttggaatgt gtaattaaat taagtttggt tattcgatgg
      181 aaggcaaatt tgatataaaa ctccatttaa agtgccagat aaaaacttta tggttatata
      241 aaagttaaaa gtaatttata gtgttataga acattcaaag ggatttaaag taatctatgg
      301 tgttttgaaa tattcaaagg gatttaaact aatctatagt cttgtagaat attcaaagag
      361 atttaaaaca atctataatg ttgtaaaata tattcaaagg gatttaaact aatctatagt
      421 cttgtagaat attcaaagag atttaaagca atctataatg ctgtaaaata tattcaaggg
      481 gatttgaagt aatttattgt gttttgaaat attcaaaaag atttttggaa tagtttttat
      541 aaatttacca gtactaataa ttttatgttt aacatttaga tgacctaaaa tcaactattt
      601 gcacttttgt attcatctaa aatcatttta taatattttt aaataatgga aaggatgttt
      661 gaatatgttt gaatcaagat gtggtgtctg ttgtgacagt tgtacaaaaa aagaacaagt
      721 aaattgcact ggttgcccaa ctatggaaaa accattttgg ggtggagaat gtaaggttaa
      781 aacttgctgt gaaagtaagg aacttaatca ttgtggtgaa tgtgatactt tcccttgtga
      841 tatgctttta aataagggaa aagaccaagg ttttgaccct atggtaaata ttgagcaatg
      901 tagaaaatgg cttgaagaaa cagtttctaa ttaattgttt tagaatgctt tgttaaggaa
      961 tttatttaat cctttgttag gtagtatgtt tagtcatttg ttgattagta tatttataca
     1021 tttattgatg ggtatattta gccatacaaa tagatttaca taaaatttac gactaaaaaa
     1081 gagttgagta ttagttttta ttatttaaaa cggatattca actctttttt atgtctaact
     1141 tttttgattt tttaatcttt ccaataccac cactttaatt tttctggttc tggattttca
     1201 gttgatgcaa aatatactgc acatctgtat acatataact gacatctgtc tatgtgctgt
     1261 ccacgcatta aacattcctt ttcatacatt tgtgctgggt ttgcatcttt aagtgattta
     1321 atagttgtat atcctaacat gattaaagat ttttctgttg ctttccctac ccctggaatt
     1381 ttttgtagtt ctcccatact tcaccttctt tctggtatat tctttttata ttatacctat
     1441 taccagatat ttttttatta tagttaatat ttaatattta attttttatt atatcaattt
     1501 atttatgctc tttcatatat atatatttta ccacctctaa atcactgagt cacttaatta
     1561 catcataata tagttttata caaataaaat actttatgtt taatttaata tataaaattc
     1621 accttcaaga aaattatatt ataatctgac atttttatct tattttttca aaatatattg
     1681 aatcttctta ctttattttg taaaattatg cttaggggaa atatatttta ggaaaatatg
     1741 aatatataat ttttagtcaa ctagttattt taagttttta acttttaaaa taaaatatat
     1801 ctaataaaag ggagattgta ttatgttttc taaaaaaaat gagggtaacg aatttagtaa
     1861 tgaaggaaaa ggaagctcta agaaaataat taaattcttt aagagcacaa agggtattgc
     1921 tctactggca tttattttag gcgtgttttt tggcaatata tcctcaccat cttgttctga
     1981 agaccatgag gaggtcattt ctaaccaaac atcagttata gattctcaaa aaacagaaat
     2041 agaaacttta aatagcaaat tgtctgatgc tgaaccatgg ttcaaaatga aagacgacga
     2101 aaagaaagct attgaagctg aaaatcaacg taaagctgaa gaagctaaaa aagctgaaga
     2161 agctaaaaag gctgaagaac aacgcaaaaa agaagaagag gagaagaaag gatatgatac
     2221 tggtattact tatgaccaat tagctagaac acctgatgat tataagtaca aaaaggtaaa
     2281 atttgaaggt aaggttattc aagttattga agatggtgat gaggtgcaaa taagattagc
     2341 tgtgtctgga aattatgata aggttgtact atgtagttat aaaaaatcaa taactccttc
     2401 aagagtatta gaggatgatt acataactat aagaggtata agtgctggaa ctataactta
     2461 tgaatcaact atgggtggaa atataactat accagggata gctgtagaga aaattaatta
     2521 atattgtttt gtattatagt taatatttta tattatagtc aatatgttta aagatgtttt
     2581 tataattgca aataaacagt tacaaggctc taaattagtt tttgctttta gcatattatc
     2641 tattttttat caactattaa ttattt
//
LOCUS       DBGJFF010000251         2659 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.256, whole genome shotgun sequence.
ACCESSION   DBGJFF010000251 DBGJFF010000000
VERSION     DBGJFF010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.256"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            696..2561
                     /gene="ltrA"
                     /locus_tag="ACOIAF_003822"
     CDS             696..2561
                     /gene="ltrA"
                     /locus_tag="ACOIAF_003822"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HGZ2670797.1"
                     /translation="MNRVKTSKTLRHSEYYDMTETFDRLYEASQNQQIFVDIMSVIMS
                     DENIMLAYRNIKKNKGSNTKGTDKQTIQNIELMSEKEFVQKIKDMFCCYTPKPVKRVN
                     IPKPNGKLRPLGIPTIWDRIIQQCVLQVLEPICEAKFHNNSYGFRPNRSAHHAIAQCY
                     RLMNQSKLQYVVDIDIKSFFDNVNHAKLIRQMWTMGIRDKHLLGIIRAMLHAPIILTD
                     GKIEHPKKGTPQGGILSPLLSNIVLNELDWWIASQWEEIPTKSKSARVLDRRDKGKGI
                     DKGNKYKELRKSTLKEIYIVRYADDFKIFCRKRKDADKIFHAVIQWLKQRLSLDISEE
                     KSKIVNLRKQKSEFLGFEMKLVRKADKYVVQSHMSKKAMNKAENNLKEQIKRIQHPAN
                     DREQAKEISIYNSMVVGIHNYYNKATLIAFDCSKVNYIISNWIDNRLNVTKQGAIQNK
                     YLSKKYGKSQQIRWLNNCPIIPIGYVKHSKAISIKNEICKFTPKGRDLIHKSLDIDVG
                     VLVQLMKNPILDMSVEYNDNRISKYTAQLGRCAVLGVSLSFQEIHCHHIKPISDGGTD
                     KFQNLIIVHESIHKLIHATSQEIISKITSKFKLNKKQISKINALRIKVNNGVITV"
BASE COUNT         1033 a          420 c          528 g          678 t
ORIGIN      
        1 ctgaaaaggt caggcactta aatctgaaag cgtaataatc gttttccttt aaggagaact
       61 gataactcaa agggtggaat gattgggtaa cgccttgaaa cgccctctct gatactccga
      121 ttagcattta ttttatagca gataagtgtt aaaagctcgg tgaagtcggc tgaaagttac
      181 cgtaaatata caaaatggat atacgaaacc tgttcagagg tggacggtgt aactgatagg
      241 tcgggagtct ataaactgtt cacggtgaga atgaaattac tgacgaactt gcgaatgaaa
      301 gagtctataa catgacggta tagaaatgta tcgggttcta taaaacggac gcatatcgtg
      361 gttgagtaaa aatcgtagat atgaaaaacc atgcagtgtt acaggcgttg gagatatgca
      421 ggcttaaagc aagcacctaa aaacagatgt aaagatagag ttatcggaac gtggaaagct
      481 gacaacgggg agattgctat aaatctatga aacggttgct aggaatacat aatagcattt
      541 atgtcagtga aagtagtggc acagtaccta agaagccata gaaatatggt ggagggatag
      601 ccacaagtca gtctttaaac ttaactatgc tatatacttc atagaatcga gtaagacaaa
      661 ataaacacca aatcctaata gaaaggactg gtgctatgaa tagggtaaaa acaagcaaaa
      721 cacttcgtca tagtgaatat tacgatatga cggaaacctt tgaccgacta tatgaagcaa
      781 gtcaaaatca acaaattttc gttgatatta tgagcgttat catgtcagat gaaaatatca
      841 tgctggcata cagaaacatt aaaaagaata aagggagtaa tactaaaggg acagataaac
      901 agaccataca aaacatagaa ctgatgtctg aaaaagagtt tgttcaaaaa attaaagata
      961 tgttctgctg ttatactcca aaacctgtta agcgagtgaa tatacctaaa ccaaacggaa
     1021 agttacgtcc gttaggaata ccgaccattt gggatagaat tattcagcag tgtgtcctac
     1081 aagtattaga acctatttgt gaagcaaaat tccataacaa tagctatggg ttcagaccca
     1141 atcgctcggc tcatcacgcc atagctcaat gttacagatt gatgaaccaa agcaaattgc
     1201 aatacgttgt ggatatagac attaaatcat tttttgataa tgtaaatcat gcaaaactta
     1261 ttaggcaaat gtggacaatg gggatacgag ataagcattt actaggaatt atccgtgcca
     1321 tgcttcatgc tcctattatt ctaacagacg gaaaaataga acacccaaag aaaggaactc
     1381 cgcaaggagg aatactctcg cctttactat caaatattgt acttaatgaa ttggactggt
     1441 ggatagcttc gcaatgggaa gaaattccaa ccaaaagtaa atcagcacga gtattagata
     1501 ggcgagataa aggaaaagga attgataaag gcaataagta taaagaattg cgtaaatcaa
     1561 ccctaaaaga aatttatata gtacgttatg cagacgattt taagatattc tgtcgaaaga
     1621 ggaaagacgc tgataaaatc tttcatgctg ttatacaatg gttaaaacaa aggctttctt
     1681 tagatataag tgaggaaaaa tcaaaaatcg taaatcttag gaaacaaaaa tcagagtttc
     1741 ttggatttga gatgaaattg gttcgcaaag ctgataaata tgtggtgcaa tcccatatga
     1801 gtaaaaaagc tatgaacaaa gcggaaaaca atctaaagga gcagattaaa cgaatacagc
     1861 accctgctaa tgacagagaa caagcaaaag aaatcagtat ctataactct atggtagtag
     1921 gcatacataa ctactataat aaggcaacgc tcatagcctt tgactgttca aaagtcaatt
     1981 acattataag caactggatt gataacagat taaatgtaac aaaacaagga gctattcaaa
     2041 acaaatatct atcaaaaaaa tatggaaaaa gtcagcaaat acgctggtta aacaactgtc
     2101 ctattattcc gattggatac gtaaaacaca gcaaagctat atctattaaa aatgaaatat
     2161 gtaagtttac gccgaaagga cgagatctaa ttcataaatc gcttgatata gatgtagggg
     2221 tacttgttca acttatgaaa aatcccatat tagatatgag tgttgaatat aatgataacc
     2281 gaatttctaa atatacagcc cagcttggaa gatgtgctgt actaggagtt tcactatcct
     2341 ttcaagaaat ccattgtcat catataaaac ctatttcaga cggtggcacg gataagtttc
     2401 aaaatctaat tatcgtccat gagtctatac acaagctgat acacgcaaca tcacaagaaa
     2461 taatcagcaa aataacaagc aaatttaaac tcaataagaa acaaatatca aaaatcaatg
     2521 cgttgagaat aaaggtcaac aatggtgtta ttactgttta acttgaaaaa tatatagcaa
     2581 gtaagaaaaa gattgatgga acgccgtgtg cggtgaaagt cgcacgcacg gtgtggagcg
     2641 ggggaaaact cggagatta
//
LOCUS       DBGJFF010000252         2654 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.257, whole genome shotgun sequence.
ACCESSION   DBGJFF010000252 DBGJFF010000000
VERSION     DBGJFF010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.257"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            56..1180
                     /locus_tag="ACOIAF_003823"
     CDS             56..1180
                     /locus_tag="ACOIAF_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 domain-containing protein"
                     /protein_id="HGZ2670798.1"
                     /translation="MSKKTKYFSLILFPIALLLNFIASKVPNIVEKYYSQFIDKIIVQ
                     ILSKVSGIFPFSLYEITMYIIVISIFLFLCCTLSTIFNKKKKLNIYLKNSILNILSIV
                     SIVYFLFVVLWGINYNRIPLETTLINNYNLKNNTSIQSKQYSVKELTKLYKFLVIKAN
                     ETRKLTLQDKNGIVKSNTDYNGVINRAQLGYDNILDILPSVSGSYSKPKYIISSNLMC
                     YTGITGIYFPFTGEANVNIAIPDLYIPCTVEHEMAHQRGFASEDEANFIAYLTSIKHP
                     NIDFNYSGYILALNYTASALSKVDYNAYVDISAGISDSVRRDLKNESEFWQKYEGKIN
                     EISNEFNNSYLKANGVTEGTQSYGKMVDLLLTYYELYPYN"
     gene            complement(1654..2478)
                     /locus_tag="ACOIAF_003824"
     CDS             complement(1654..2478)
                     /locus_tag="ACOIAF_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5780 domain-containing protein"
                     /protein_id="HGZ2670799.1"
                     /translation="MRYKKISSLPILVVALLITGTTTTFSLSTTNSEKTNVYLANTVS
                     TVTKKVSDIQSSKGLVYNENIPLFINNSKIQYDDTPYHWPSNVISLTNSSEKAIMDYE
                     ITCLAYDKNGKPLELYWDAQNVAADGEVGSVGFSPAGVDYGIVTGISPVSPKSYSHTY
                     RKMQQSPPQDIISMFEKQQGKAWVENWLKEWKQMEKEYAKQNAIAPGKNQNDAFLLFD
                     KWKQSTGEHGVKYIISCVKQVTFNDGSVWKNSAYENWLKSFQGKEVSNSVLENYYK"
BASE COUNT          933 a          404 c          294 g         1023 t
ORIGIN      
        1 tttcttcaat tgatatataa tatgtttata taaactaatg tatgtgaggt gaaatatgag
       61 caaaaaaaca aaatattttt ctttaattct ctttcctatt gctttgcttt taaattttat
      121 agccagtaaa gttccaaata ttgttgaaaa atattactcc caattcatag ataaaataat
      181 tgtacaaata ttgagtaaag tttctggaat tttcccattt tctctctatg agattacaat
      241 gtacataata gttatatcta tcttcttatt tttatgttgt actttatcta caatttttaa
      301 taagaaaaaa aaattaaaca tatatttaaa aaattccatt ttaaatatat tatccattgt
      361 atcaatagtc tactttcttt ttgtagtgct ttggggaata aactataata ggattccact
      421 agaaaccaca cttataaata actataattt aaaaaacaat acctctatcc aatcaaaaca
      481 atatagtgtt aaagagctta ctaaattata taaattcctt gtaattaaag caaatgaaac
      541 tagaaaactc acattacaag ataaaaatgg tattgtaaaa tctaatacag attataatgg
      601 tgtcataaat agagctcaac taggatacga taatatcttg gatatattac ctagtgttag
      661 tggtagttat tctaagccta agtatattat ttcatcaaat ctaatgtgct atacaggaat
      721 aactggtata tactttcctt ttacaggtga ggcaaatgta aatatagcaa tacctgactt
      781 atatattcct tgtacagttg aacatgaaat ggcacatcaa aggggatttg ctagtgaaga
      841 tgaagctaat tttatagcgt atcttacttc tataaaacac cccaatattg attttaacta
      901 ttctggatat attttagctt taaattatac tgcttctgct ttaagtaagg ttgattataa
      961 tgcatatgtt gatatttctg ctggcatttc agattctgtc cgtagagatt taaaaaatga
     1021 aagtgaattt tggcaaaaat atgaaggtaa aattaatgaa atttctaatg agtttaacaa
     1081 ttcttactta aaagctaatg gcgttactga aggtactcag agttatggta aaatggttga
     1141 tttattgcta acttattatg aattatatcc atataactaa acactacaat tcaaaatttt
     1201 attcatatac agctgatatt aaatcagctg ttttctattt aaataaatta tttaacttca
     1261 agcaatctta tataatttga tagtacttat actagctgta taacatttca attctaacta
     1321 ttttagttaa aacatttcta ttatcttttt taataaatgt tttaactaaa atttttaaaa
     1381 cataaaactt ccacattatt tcactatcta tataaaaatc taaaaattca catataaaat
     1441 tacataacta aaaaatcata aatttataga aagcatatat tcaaaataaa aaagcatgta
     1501 tcaaaacttg acacatgctt ataatcttat gtatatatat cattttgtca caataaaaat
     1561 ttccttgtaa cactgatatt tttaaacaaa atagtcctta tttaattaaa ttaacattaa
     1621 ttaaattcgt actcattttg ctatcattaa acattactta tagtaatttt ctaatacact
     1681 atttgaaact tccttccctt gaaaactttt caaccaattc tcatatgctg agtttttcca
     1741 tacactacca tcattaaatg ttacttgttt tacacaagat ataatgtact ttacaccatg
     1801 ctctccagtt gattgtttcc atttatcaaa caatagaaag gcatcatttt gattcttacc
     1861 tggtgctatt gcattttgct ttgcatattc tttctccatt tgtttccatt ctttcaacca
     1921 attttctacc catgccttgc cttgttgttt ttcaaacata cttattatat cctgtggtgg
     1981 tgattgttgc atttttctat aagtatgtga atatgatttt ggagacacag gagatatgcc
     2041 tgtaacaata ccatagtcta caccagctgg gctaaatcca acacttccta cttcgccatc
     2101 tgctgccaca ttttgagcat cccaatatag ctcaagtggt tttccatttt tatcatatgc
     2161 taagcatgta atttcataat ccataattgc cttttcactg ctatttgtta agcttataac
     2221 attgctaggc caatgatatg gagtatcatc atactgtatt ttgctattat ttataaataa
     2281 tggaatattc tcattataaa caagaccttt actactttga atatctgata ccttctttgt
     2341 gacagtactt accgtatttg ctaagtatac atttgttttt tctgaattgg tcgtactaag
     2401 agaaaatgtt gttgtcgttc cagtaatcag caaagcaaca actaaaatag gtaagctaga
     2461 tattttttta tatctcacaa ttcttcacct ctttttctat taacttataa taaaacaatt
     2521 taccaaaaca ttaaaatcta tgttaacata atttaccaac tgttctagta actatatcct
     2581 atcatagtta ctatcaagtt tcaatactat tgatggaaat tttcactata ttaaatcttt
     2641 ttttaatttt ataa
//
LOCUS       DBGJFF010000253         2634 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.258, whole genome shotgun sequence.
ACCESSION   DBGJFF010000253 DBGJFF010000000
VERSION     DBGJFF010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.258"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            296..2431
                     /gene="rnr"
                     /locus_tag="ACOIAF_003825"
     CDS             296..2431
                     /gene="rnr"
                     /locus_tag="ACOIAF_003825"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HGZ2670800.1"
                     /translation="MIPGLKESLLGLINESAYNPLKKEELAEIFDIHSSEMPMFYNFL
                     NELEEDGYICFTKKGKIVSPNQMGYFVGKFVSHKKGFGFVESDVEYTQDLFISSDNIN
                     GAMHNDRVMAEIVVPATEEKRAEGRIVKVIKREITRIVGTFQSSKTFGFVTPDNKKFT
                     KDIYIPKKHFSGAVDNDKVVCEITVWPQEDRKPEGKIIEILGQKGERGVEIDSIIREH
                     GLPEEFPKKVLQEAEAVAVEIPQEEIKRRRDLRDLNIFTIDGDDAKDLDDAISIEVLP
                     NGNFKLGVHIADVTHYVREKNKLDKEALKRATSVYLVDKVIPMLPKTLSNGVCSLNPF
                     EDKLTLSIFMEIDHKGNVVKHEICESVINSKARMTYTEVSDILEKDDEKLKKTFEHVV
                     EDFKNAEILARILMSRREKRGAIDFNFPEAKIILNGKGEVVDIKPYERRISNKIIEEF
                     MLISNETIAEHFYWMGIPFVYRIHETPSLEKMEELSKFISTFGYIIKGDKEEVHPKAL
                     QGIIEKIKGKKEEGAISTIMLRSLKQAKYSPECSGHFGLAAKFYCHFTSPIRRYPDLQ
                     IHRIIKESLNNKISGKRQEQLTTIVDYASVQSSEKERKAELAERDVHDFYKALYMKDK
                     VGQEFEGVVSSVTSFGIFVELDNTIEGLIRLANMNDDYYIYDESSYSVLGERTKKSYK
                     IGDLVKIKVESVNVDFKEIDFEIVEKIEE"
BASE COUNT         1137 a          259 c          484 g          754 t
ORIGIN      
        1 taccaatcct atttgatggt gtataattag gtgataaaga gtaatttaag caattatgat
       61 aaagaataac ttaagtaatt ataatgatat ttacaaataa tttataaaac atcataattt
      121 gtttgaagtt agtatgacaa agcttacatt aaatcaaaaa tataaataag taattttaac
      181 ttataataat aagaaaatat agaaaattat gtgataagta aaaaatgttt tgtctgaaga
      241 aatataaaag acaatataaa taataatata atgaaaagta aaacaggagg gaaaaatgat
      301 accaggactt aaagaaagtt tgctaggatt aataaatgaa agtgcatata atcctctaaa
      361 aaaagaagaa ttagcagaaa tatttgatat acattcatca gaaatgccaa tgttttataa
      421 ttttttaaat gaattagaag aagatggtta tatctgcttt actaaaaaag gaaaaatagt
      481 atctcccaat caaatgggat actttgttgg aaaatttgta tcacacaaaa agggttttgg
      541 atttgtagaa tctgatgttg aatatacaca ggatttattt atctcaagtg acaatataaa
      601 tggtgctatg cataacgata gagttatggc agaaatagta gtacctgcaa ctgaagaaaa
      661 gagagcagaa ggaagaatag taaaagtaat aaagagagaa ataactagaa tagtaggtac
      721 tttccaatca agtaaaactt ttggatttgt aacaccagac aacaagaaat ttactaaaga
      781 tatatatata cctaaaaaac attttagtgg agcagtagat aatgataagg tagtatgtga
      841 aattacagta tggccacaag aggatagaaa accagaaggc aaaattatag agatactagg
      901 tcaaaaaggt gaaagaggcg tagaaataga ttctataata agagaacatg gtcttccaga
      961 agaatttcca aagaaagtat tgcaagaagc agaagctgta gctgttgaga ttcctcaaga
     1021 agagataaaa agaagaagag atttaagaga tttaaacata tttactatag atggagatga
     1081 cgcaaaagac ttagatgatg ccatatcaat agaagtatta cctaatggaa acttcaaact
     1141 aggagttcac atagctgatg ttacacatta tgtaagagag aaaaataaac ttgacaagga
     1201 agccttaaaa agagctacat cagtttattt agtagataaa gtaattccaa tgttgccaaa
     1261 aactctttca aatggagtat gtagcttaaa tccatttgaa gataagctaa ctctttctat
     1321 atttatggaa atagaccata aaggaaatgt ggttaaacat gaaatatgtg aatctgtaat
     1381 aaattcaaaa gctagaatga cttatacaga agtatctgat atattagaaa aggatgatga
     1441 aaaacttaaa aagacttttg aacatgttgt tgaagatttt aaaaatgctg aaattttagc
     1501 taggattctt atgtcaagaa gggaaaaaag aggagctata gacttcaatt tcccagaggc
     1561 aaagataatt ctaaatggaa aaggcgaagt tgtagatata aagccatatg aaagaagaat
     1621 atctaataag ataatagaag aatttatgct tataagtaat gagacaattg ccgaacattt
     1681 ttactggatg ggaataccgt ttgtgtatag aatacatgag acaccatctt tggaaaaaat
     1741 ggaagaattg agtaagttta tatctacatt tggatatata ataaaaggtg ataaagagga
     1801 agtacatcca aaagcattac aaggtataat agagaaaata aagggcaaaa aagaagaagg
     1861 agctatcagt actataatgc ttcgttcttt aaaacaagct aagtactcac cagaatgtag
     1921 tggacatttt ggattagcag ctaaattcta ctgtcacttt acttcaccaa taagaagata
     1981 tcctgacctt caaattcata gaataataaa agaatcttta aataataaaa taagtggaaa
     2041 aagacaagaa cagcttacta ctatagtgga ttatgcatct gttcaatcat ccgagaaaga
     2101 aagaaaggca gagttagctg aaagagatgt acatgatttc tataaggctc tttatatgaa
     2161 ggataaagtt ggtcaagaat ttgagggtgt tgtatctagc gtaacttcat ttggaatatt
     2221 tgttgagtta gataatacca tagaaggatt gataagactt gcaaatatga atgatgatta
     2281 ctatatttat gatgagagtt cttatagtgt tttaggagaa agaactaaga agtcatataa
     2341 gataggagac cttgttaaga taaaggttga aagtgttaat gtagatttta aagaaattga
     2401 ctttgagata gttgaaaaaa tagaggagta gataaaacag ctaaaataag agataacaag
     2461 ttgtagtatt tatgatatgt ataaatatat agtgcacaat actctataaa ataaatgata
     2521 aaaataggta attgagaaat atctaatgga aatagttaac tgaaagataa ataattaaga
     2581 aatatttaat gaaagtagct aattgaaaaa taagtaattg agaaatgttt aata
//
LOCUS       DBGJFF010000254         2634 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.259, whole genome shotgun sequence.
ACCESSION   DBGJFF010000254 DBGJFF010000000
VERSION     DBGJFF010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.259"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            718..2538
                     /gene="ltrA"
                     /locus_tag="ACOIAF_003826"
     CDS             718..2538
                     /gene="ltrA"
                     /locus_tag="ACOIAF_003826"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HGZ2670801.1"
                     /translation="MLKKDKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKTNSGSKTAGTDGLTIDDIKHLCDEDIIMKVRSSLDNYQPKSVRRVFIPK
                     SGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHALGRVTSL
                     INISKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKHRNKNGWFQYAKKHTRLKSGFI
                     VRYADDFKIMCSTYEEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGKTKHGYVAKTDMNQKALKKAKTNLKLKVKDIVRHTTTFQIARYNLAVMGMQN
                     YYCVATNIYNNLTEVSYALLPTTRVRFKKIAKLIPFETTSQDFQMKTTGIRPQTKIIM
                     IADTPLLPINGVKHKNPLNFSQDICNFTEHGRSRIHEEIALVTKGEIRILLEYKDTTK
                     SVEFNDNRISVFIAQQGNCYITNRRHSPTDMVCIYKNITETDRDKYQNLVFVEIPISK
                     AILTESVQQAKMWLMNYGLSSQQKRKLNKIRANYGYQAIK"
BASE COUNT          953 a          436 c          565 g          680 t
ORIGIN      
        1 tacttgaaaa gagtagacac tgcacaatca attaaaattg tgttggagaa ctgaacacac
       61 gaatagtcga atgatggggt aacgccctga aaggctactt taatactccg actggcagga
      121 ttatgagtaa acataggttt gtcagaagct cggtgaagtc ggcagagaga taccgtaaca
      181 gatagcgctg tcgaaagata cccagaggtg ggtgtcgtat cctatatgcc ggaagtctat
      241 aaaatatcta tggttagtat gtagatgagc ggtctactga cgaatctttg aatgtacagg
      301 tctatatcgg gactggatag aaatgtccag tgacacaaat agtgtcgcta ctgtgggtga
      361 gtaagactcg tttttatgaa aacccatata atgttacagg cattatcaaa gtagcaggct
      421 tagagaagaa acctacggat atatatgtac agatagtgtg tttggaacgt tgaaagctgg
      481 caacatggag attttactgt ctatgaagtg ttcacatcat aaggttgttg tgagattagc
      541 aataatataa ggtgtgttta tgccagtgaa agtggtggca cagtacctat gaaaccgtga
      601 taacaaacgg tggagggata gccactagtc attgaagaat agaattacta agaatggtat
      661 gggttcgagt aagactaaga gatgtacttg cccctgtgat taaggagggt acagaccatg
      721 ctaaagaaag acaagctccg ctataacgaa tattatgata tgcaacatat ttatgatgag
      781 ttatacgcac agagcaaaaa tggtaataac ttttataagt tactggaaat tattggttca
      841 gaacaaaata tctgcttggc ataccgaaat ttaaaaacaa acagcggaag caaaaccgct
      901 ggtacagatg ggctaacgat tgatgatatt aaacatttgt gtgatgaaga tattattatg
      961 aaagtccgaa gcagcctgga taattaccaa ccaaaatcag tcagacgggt ttttattcct
     1021 aaatctggaa gcgataaaat gcgacctctc ggaatcccct gtatctggga cagacttgta
     1081 caacaatgta tcttacaagt cctagaaccg atatgcgaac ctaagttcca taatcattcc
     1141 tatggattcc gtgcgaatcg aagtgcccac catgcacttg gcagagtaac cagtttgata
     1201 aacatatcaa agtatcatta ctgcgttgat gtagatatta aaggattctt tgataatgtc
     1261 aaccacggga aattgttaaa acagatttgg acacttggca ttcgggataa aagactgatt
     1321 tgcattatta gtaaaatgct gaaagctgaa attgacggcg agggtgttcc agaaaaagga
     1381 actccgcaag gcggtctgtt aagtcccctg ctctcgctca tagtccttaa tgaattggat
     1441 tggtgggtaa gcagtcaatg ggaaactttc cagccaaagc accgtaataa aaacggttgg
     1501 tttcaatatg caaagaaaca tacgaggtta aaaagtggct ttattgtgcg ttatgctgac
     1561 gattttaaaa ttatgtgttc gacctatgaa gaagcacagc gattctatca cagtaccgta
     1621 gattttctaa acaaaaggct gaaattagaa atcagtccag aaaaatcaaa ggtagtaaat
     1681 ctgaagaaaa attcatcaga ttttctggga ttcaaaatta aagtgattcc aaaagggaag
     1741 acaaaacatg gatatgttgc caaaacagat atgaaccaga aagcactaaa gaaagcaaaa
     1801 acaaatctaa agttaaaagt caaggatata gtcaggcaca caactacttt ccagattgca
     1861 cgttataatc tggcggttat gggaatgcag aattattatt gtgtggctac gaatatatac
     1921 aacaatttaa cagaagtaag ttacgctctc ttaccgacca ccagagttcg ttttaaaaag
     1981 attgcaaagc taataccttt tgaaactaca agtcaagatt tccagatgaa aacaactgga
     2041 atacgaccac aaacaaaaat tattatgatt gccgatactc ctctattgcc aatcaacgga
     2101 gtaaaacaca aaaatccgct gaatttctca caggatatat gcaactttac agaacacgga
     2161 agaagcagaa tacatgagga aatagcattg gtaacgaaag gagagatacg gattctttta
     2221 gagtataaag acaccacaaa gagtgttgaa tttaatgata atcgaatttc ggtatttata
     2281 gcccaacaag gcaattgcta tatcactaat cgaaggcata gcccgactga tatggtttgt
     2341 atctacaaga atattaccga aacagataga gataaatacc aaaatcttgt atttgttgaa
     2401 ataccaatat caaaagcgat actgactgaa tcagtacaac aggcaaaaat gtggttgatg
     2461 aactatggct tgagcagtca acaaaagaga aagctgaaca aaatacgagc gaactatgga
     2521 taccaagcaa taaaataacc agaacttatg aatctatttg aatatgatgg aacgccgtat
     2581 gaagcgaaag tttcatgtac ggtgtgaagc gggggaaaac ttggagataa catc
//
LOCUS       DBGJFF010000255         2583 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.260, whole genome shotgun sequence.
ACCESSION   DBGJFF010000255 DBGJFF010000000
VERSION     DBGJFF010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.260"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            <1..223
                     /locus_tag="ACOIAF_003827"
     CDS             <1..223
                     /locus_tag="ACOIAF_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670802.1"
                     /translation="KNYNFILLAIFFILLLIQNGVLKTGYQNRATFYQDNYFNGEDKN
                     DKIKFMTIKIFIFLSLPLCFNILFNYLTS"
     gene            complement(762..1313)
                     /locus_tag="ACOIAF_003828"
     CDS             complement(762..1313)
                     /locus_tag="ACOIAF_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HGZ2670803.1"
                     /translation="MSINTVIAKNLNRLRNERNLSLGQLAELSGVSKVMLSQIEKGDS
                     NPTVNTIWKIASGLNVPYTAILEQPQNETFIVSKTDIDVQVSENKDYRLYCYYPNTPT
                     RNFELFQMELEEGHSYTSVGHSEKSQEYIMIIEGQLKLEVNDSIYQLRENDSICFSAE
                     SIHTYHNQGEKTLKAVIINYYPV"
     gene            1504..2466
                     /locus_tag="ACOIAF_003829"
     CDS             1504..2466
                     /locus_tag="ACOIAF_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HGZ2670804.1"
                     /translation="MRVLNNVTEAIGNTHMLHLSKIADEFGVQGNIYAKMEYLNPGFS
                     KKDRVALQMITEAENAGLLVPGQAVVELTSGNTGTGLAIVCACKGYKFIACMSKGNSM
                     ERARMMKALGAEVVLVDQMPNSVHGQVSGEDLALVEIEAQKIAKERNAFRADQFNLEA
                     CNHAHEKYTAEEMWEQLDGNIDVFVDFLGSGSTFAGCSKTLKKHNSSIKCYVVEPETA
                     AIYSGKEITDQGHKIQGGGYSMDLPLVDKKLIDGYIQVTDEEATDVARKLAKLEGVFA
                     GFSSGANVCAAIKLLKSSEKGKNIVLTLNDSGLKYLSTDLYEEL"
BASE COUNT          996 a          270 c          418 g          899 t
ORIGIN      
        1 aaaaaattat aactttattt tattagcaat cttttttata ttgctgttaa ttcaaaatgg
       61 agttttaaaa acaggttatc aaaatagagc tacattttat caagataatt attttaatgg
      121 agaggataaa aatgataaga taaaatttat gactataaag atttttatat ttttgtcatt
      181 accattatgt tttaatatac tattcaatta tttaacctct taaattatat ttagatatag
      241 gagtcttatg ataaaagtaa gcttatgtgt tatttgtagt aaatataaag tgcatatagt
      301 ggtaaaacaa aagtttcata taaatgaaga aaaaaatata aaataattta tttaactact
      361 taaaaataaa ctaaaaggga taataaaata gcttttaggt tatggtaata aaggtattta
      421 aaaagagtgc ataagcactc caattgtgag aaatacatac acaaatcatt attacaacaa
      481 tgaaaaatgt acatataaga gacgttttgt atttgtttta agtgataata ttttaatact
      541 aactaaataa ttatcacaat ggtaaaaaag aaaaaggagt attttgtact ccatttgtca
      601 aaaatataaa acttttatat aagcttatta taacataatt gataaaagtg taaagatata
      661 taatgaattt ataaagaaaa ataaaataaa aaagataaat cctaatcaaa ggatttatct
      721 ttttgtatag aaaataatta ttctattatt aaatggcatg gctaaactgg ataataattt
      781 attattactg ctttcaatgt tttttctcct tgattatggt atgtatgaat tgattcagct
      841 gaaaaacaaa tagagtcatt ttctctaagt tgataaatag agtcatttac ttctaatttt
      901 aattgacctt ctataatcat aatatattcc tgtgattttt cagaatgacc aacagatgta
      961 taactatgac cttcttctaa ttccatttga aataattcga agtttctagt tggtgtattg
     1021 ggataataac aataaagacg ataatcttta ttttctgaaa cttgtacatc tatatcagtt
     1081 tttgatacaa taaaagtttc attttgaggt tgttccagta ttgcagtata tggaacattt
     1141 aatccacttg caattttcca aattgtgttt actgttgggt ttgagtctcc cttttcaatt
     1201 tgcgaaagca ttactttact tacgccagat aattcagcaa gttgacctag acttaagtta
     1261 cgttcattac gtaatctatt taaattttta gcaataacag tattaatact catagtagct
     1321 ccttaaaatg taaattattg acaaatataa tttacgaatg tataatttat ttaacattat
     1381 ttataattta caaatgtata atataatgaa cgttaaatac actgtaaatc cgtaatataa
     1441 attatactgc ataaagagtt gtttttcaac ggatgatagt tgagagatta agggggaatt
     1501 gtaatgcgtg tacttaataa tgttacagag gctataggca atactcatat gttacattta
     1561 tccaaaatag cagatgaatt tggagttcaa ggtaatattt atgcaaagat ggaatactta
     1621 aatccgggat ttagtaaaaa ggaccgtgta gcattacaaa tgattaccga agcagaaaat
     1681 gcaggtttat tagttccagg tcaagcagtt gtggaattaa caagtggtaa tactggaaca
     1741 ggactagcaa tagtttgtgc atgtaaagga tataaattta tagcgtgtat gtcaaaagga
     1801 aattctatgg aaagagcaag aatgatgaaa gctttaggtg ctgaagtagt tcttgtagac
     1861 caaatgccta attcagttca tggacaggta tctggtgaag atttggcact tgtagaaatt
     1921 gaggctcaaa aaatagcaaa agaaagaaat gcatttagag cagaccaatt taatttagaa
     1981 gcttgtaatc atgcacatga aaagtataca gcagaagaaa tgtgggaaca attggatgga
     2041 aatattgatg tatttgtaga ttttcttgga agtggtagta cttttgcagg atgttcaaaa
     2101 acacttaaaa aacataattc ttctatcaaa tgttatgttg ttgaacctga aacagcagca
     2161 atttattcag gtaaagaaat aactgaccaa gggcataaaa tacaaggtgg aggatattct
     2221 atggatttgc cattagtaga caagaaactt attgatggtt atattcaagt tacagacgag
     2281 gaggcaactg atgtagcaag aaaattagct aaattagaag gtgtatttgc aggattttct
     2341 tcaggggcaa atgtatgtgc agcaattaag ttattaaaga gcagtgaaaa aggaaaaaat
     2401 atagttttaa ctcttaatga tagtggttta aaatatttaa gtactgattt atatgaagaa
     2461 ttgtaataat agcacaatag ttatgcaatg ttttttaaat taagtatata gatttaaatt
     2521 ataaaaagta tctcaatgta atttttgaaa gttataaagg agatactttt ttattttgta
     2581 aat
//
LOCUS       DBGJFF010000256         2521 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.261, whole genome shotgun sequence.
ACCESSION   DBGJFF010000256 DBGJFF010000000
VERSION     DBGJFF010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.261"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            279..>2521
                     /locus_tag="ACOIAF_003830"
     CDS             279..>2521
                     /locus_tag="ACOIAF_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HGZ2670805.1"
                     /translation="MKIPKKIAAMLTVTMIAGSSTAGIASAQTVATNLTGQERYETAV
                     KISQDGWKNADEVVIVNDSSIADALSATPFAKAKNAPILLTSKDKLNDKTKAEIQRLK
                     AKKVYLIGGTSVLSTNIEKEIKNLKISFERISGAERYQTSLELAKKLDAISDVKKIAV
                     VNGEKGLADAVSVGAPAAQNNMPVILADSKNGTAVADKFIKDAGITQSYVVGGESSIS
                     EAVKNKLPNSTRLGGTDRNDTNAKVIKEFYKKTDLKNAYVTKDGMNKQDQLIDALAVG
                     VLGAKNQSPVVLVGKNLSASQKSLVNSKSFDKITKVGGNGNETAFNEMKSLQEVKTVE
                     AKTISELKSAIDKATANDVINFKPTSEVKEAFTIQTDKAVTVNLNGTYTKTVTINMPN
                     GDVNNYAKVDDVVIDDVKDGTFVNYGKITNLKVNDKNGAKIENNSKGEIGSLTVASGA
                     SQVKVTNGGKITTVTNNSKGTTIDNKGTISSVKGDNSPTISGNSPSSNSSGGSSSSGG
                     SSHGGGSSSGGSSSNQTSVNNEAAKITSVPTPAKDATRLTMPSVSSGYTIAIKTSSNE
                     SVIKKDGTIIPPNTATTVKLVFTVTHTSSGKTADTKELSVTVPAKSTDEELQAALDNE
                     VAKITSVPAPAKDATKLTMPSVSSGYTIAIKTSSNKSVIKEDGTIIPPNTEETVTLVF
                     TVTQESSGKTADTGEIDVVVPAKSTKVIIGEGSVSGATAGDKEITGLTSGKIYKVTMD
                     GVEKYTRA"
BASE COUNT         1102 a          335 c          451 g          633 t
ORIGIN      
        1 tacttttttt atacaaaacc ctatttttta taacaattca ttaacataaa aatcaaactt
       61 accttaaaat ataaatttac aaatttttca atcaataaga aaatttcttt attccttaca
      121 taatgactgc aatgtaacaa aataattaca aattaattat gaaatcatta tagttagaaa
      181 cctctttaaa attagatatt aagtatcctt tagaattaga acgggaacaa agttaaaaaa
      241 ttaagtaata tcaaatggac aagggggaag aaaataaaat gaaaatacca aagaagattg
      301 ctgcaatgct tacagttaca atgatagctg gaagttcaac agcaggcata gctagtgcac
      361 aaactgtggc aacaaattta acagggcaag aaagatatga aactgctgtt aaaataagtc
      421 aagatggatg gaaaaatgct gatgaggtag taatcgtaaa cgactcatct atagcagatg
      481 ctctatctgc aacaccattt gcaaaagcaa aaaatgctcc aatactttta acaagtaaag
      541 ataaattaaa tgataagacg aaagcagaaa tacaaagatt aaaagctaaa aaagtatact
      601 taataggtgg aacttctgta ttaagtacaa atatagaaaa agaaataaaa aatttaaaaa
      661 tatcttttga gagaatctct ggagctgaga gatatcaaac atccttagaa ttagcaaaga
      721 aattagatgc tataagtgat gttaagaaaa tagctgtagt taatggtgaa aaaggacttg
      781 cagatgctgt aagtgtagga gcaccagctg cacaaaacaa tatgccagta atacttgctg
      841 attctaaaaa tggaacagca gttgcagaca aatttataaa agatgctgga ataactcaat
      901 cttatgtagt aggtggagag agttcaattt ctgaagctgt taaaaataaa ctaccaaata
      961 gtacaagact aggtggaaca gatagaaatg atacaaatgc aaaagtaata aaagaatttt
     1021 ataaaaagac agatttaaaa aatgcttatg taactaaaga tggtatgaat aaacaagacc
     1081 aacttataga tgcactagca gtaggtgtat taggtgctaa aaatcaatca ccagtagttt
     1141 tagttggaaa gaacttatca gcttcacaaa aatctctagt taactctaag agttttgata
     1201 agataactaa agttggtggg aatggtaatg agacagcatt taatgaaatg aagtcattac
     1261 aagaagtaaa aacagttgaa gctaaaacaa tttctgaatt aaaatcagct atagataaag
     1321 ctactgctaa tgatgttata aactttaagc caactagtga ggttaaagaa gcatttacaa
     1381 tacaaactga taaagcagta acagtaaact taaatggtac atatacaaag actgtaacta
     1441 tcaacatgcc taatggagat gtaaataact atgcaaaagt ggatgatgta gttatagatg
     1501 atgtaaaaga tggaactttt gttaactatg gaaaaataac taacttaaag gttaatgata
     1561 aaaatggagc caagatagaa aataattcta aaggtgagat tggtagttta acagtagcat
     1621 ctggagcaag tcaagtaaaa gtgactaatg gtggtaagat aactacagtt acaaataatt
     1681 ctaaagggac tactatagat aataaaggta caataagtag tgttaaggga gataattctc
     1741 caacaataag tggaaatagt ccaagttcta actcttcagg aggaagttca tcaagtggag
     1801 gttcttcaca tggaggaggc tcatcttcag gaggaagttc ttctaatcaa acatcagtaa
     1861 ataatgaagc agcaaaaata acaagcgtgc ctacaccagc aaaagatgca actagattaa
     1921 ctatgccatc agtatcaagt ggatatacta tagcaataaa aacatcaagt aatgagagtg
     1981 ttataaaaaa agatggaaca ataatacctc caaatacagc aacaacagta aaattagtat
     2041 ttacggtaac acatacatca agtggtaaaa cagcagatac aaaagaatta tctgtaacag
     2101 taccagctaa atcaacagat gaagaattac aagcagcatt agacaatgaa gtggcaaaaa
     2161 taacaagcgt tcctgcacca gcaaaagatg caactaaatt aactatgcca tcagtatcaa
     2221 gtggatatac tatagcaata aaaacatcaa gcaataagag tgttataaag gaagatggaa
     2281 caataatacc tccaaataca gaggaaacag taacattggt atttacagta acacaagaat
     2341 caagtggtaa aacagcagat acaggagaaa tagatgtagt agtaccagcc aaatcaacaa
     2401 aagtaataat aggagaaggt tcagtatcag gagcaacagc aggagataaa gaaataacag
     2461 gattgacatc aggaaagata tataaagtaa caatggatgg agttgaaaag tatactagag
     2521 c
//
LOCUS       DBGJFF010000257         2423 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.262, whole genome shotgun sequence.
ACCESSION   DBGJFF010000257 DBGJFF010000000
VERSION     DBGJFF010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.262"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            172..1065
                     /locus_tag="ACOIAF_003831"
     CDS             172..1065
                     /locus_tag="ACOIAF_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HGZ2670806.1"
                     /translation="MKNKKFIVIILIISILIGVLVKEYSSREIKKDDINVSKYIKYAD
                     LASKNNAQVNWKYVASIVAVLNKNNLKNVKDSQIQEVSDLFVKNFSKNNKINKLSDIL
                     DELEFSNRQKRLVDNYIDNLKDYGIKPERLKSDTKYMKFIDEIKTEAIQNYKDYKILP
                     SITIAQAIIESSWGKSTLAKQYNNLFGIKADAYWKGKSVTLETKEHLDTIIDDKFRIY
                     DDKNESIKDHAKFLATNKRYKNNGVFDAKTYIYQAKALEKAGYSTAKDENGNSIYAAR
                     LIELIQQYNLQLIDSEIQSEM"
     gene            1508..1999
                     /locus_tag="ACOIAF_003832"
     CDS             1508..1999
                     /locus_tag="ACOIAF_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670807.1"
                     /translation="MYENNKVIYNLERCEKLEKTLYLSQFNTYKNFKLASKKTVINSL
                     ETYVYNMNINNISLVDTIECISLEDEQLTGKKLILVGDIDIDAILDYAGNKRNRNSSK
                     KNTFKLKIPFSTFIQMPRKIENKDKINLKYLIQDITSSILDDNLFISVTAIISYENSS
                     KIE"
     gene            2027..>2423
                     /locus_tag="ACOIAF_003833"
     CDS             2027..>2423
                     /locus_tag="ACOIAF_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670808.1"
                     /translation="MRITKTTFNDVFSNDANGSIIKVEKYVDKKCASVFEILTYTIIV
                     TNTSRYKTGNIFFKDYISKYIEFINNTVKVNGIIKRGLDPQQGFYIGRIDASCKKIIS
                     FKSVVLPNSAHRIIKNSANIYYYYKCNLDK"
BASE COUNT         1036 a          223 c          352 g          812 t
ORIGIN      
        1 atttatttta actgttttgt gtgatagaaa actatatatt ataacaatag tagtgctttt
       61 aattaaatat atttcataaa tcttcataaa atcttcaaaa tttcgtaaag aatatgtcac
      121 aaaagtaagg tatattatat tcagcagata gtataaggga ggacaatatt tatgaagaat
      181 aaaaaattca ttgttataat actgataata tcaattttaa taggtgtatt agtgaaggaa
      241 tattcatcta gagagataaa aaaggatgat ataaatgtca gtaagtatat aaaatatgct
      301 gatttagcaa gtaaaaataa tgcacaagtt aactggaagt atgtagcttc cattgttgct
      361 gtgttaaata aaaataactt aaaaaacgtt aaggattcac agatacaaga agtatcagat
      421 ttatttgtta agaatttttc taaaaataat aaaataaata agttaagtga tattttggat
      481 gagttagaat ttagtaatag acaaaaaaga ttggttgata attatataga caatcttaag
      541 gattatggta taaaaccaga aagattgaag tctgatacaa aatatatgaa gtttattgat
      601 gaaattaaaa cagaagcaat acaaaattat aaggattata aaatacttcc atctataact
      661 attgcacaag ctataataga atctagctgg ggcaagtcaa ctttggcaaa acaatataac
      721 aatctatttg gtataaaggc tgacgcttat tggaaaggca agtcagtgac tttagaaacg
      781 aaagaacatt tagatacaat tatagatgat aaatttagaa tatatgatga taaaaatgaa
      841 tctataaaag accatgcaaa atttttagcc acaaataaaa ggtataaaaa caatggtgta
      901 tttgatgcaa aaacgtatat atatcaagct aaagcattag aaaaggcagg ttatagtact
      961 gcgaaggatg aaaatggcaa tagtatatat gcagctcgtc taatagaatt aattcaacag
     1021 tataatcttc aattaataga cagtgaaatt caaagtgaaa tgtaactttg attaaatttt
     1081 tattgataag gctatgattt ttaaaatcat agccttattt tgacatgctt ttacttgtaa
     1141 ttattaaatc gatatcatta tctttcaaaa tttactttat actgtaaatc aaattattta
     1201 agttaaaact tggaattatt tataaaagga ttatttactt tatctaaatt ctgcacttaa
     1261 agaacacctt ttcccttaag gctgaccgtt agtctataaa aattataatt acttataaaa
     1321 aagtgaatag agatatatat atatattttt cagaaagact tttacttgta aattgaaatc
     1381 aatgaagtaa aaaaggattc tttcttaaaa gtaaataaca actagatagt gaataataaa
     1441 aaatggtaat ttgaaataat ttttatgaat agtattaata tatagataca gggagattga
     1501 tgcgattatg tatgaaaata ataaggtaat atacaacctt gaaagatgtg aaaaactaga
     1561 aaagactctt tatttatctc aatttaatac atataaaaat tttaaattag cttctaaaaa
     1621 aactgtaata aattcattag agacatatgt ttacaatatg aatattaaca atataagtct
     1681 tgttgatact atagaatgta tatcattaga agatgaacaa cttacaggaa agaagttgat
     1741 tttagttggt gatatagata ttgatgcaat attagattat gcaggcaata aaagaaatag
     1801 aaattctagt aaaaaaaata catttaaatt aaaaataccc tttagtacat ttatacaaat
     1861 gcctagaaag atagaaaata aagataaaat taatttaaag tatcttatac aagatattac
     1921 ttcatctatt ttagatgata atttatttat tagtgttact gcaattataa gttatgaaaa
     1981 tagttccaag atagagtaat ctttaaatta tagaaaggtg gatgcaatga gaataacgaa
     2041 aactactttt aatgatgtat tttcaaatga tgcaaatgga agtattataa aagtagaaaa
     2101 atatgttgat aaaaaatgtg catctgtatt tgagattttg acctacacta ttattgttac
     2161 aaatacaagt aggtacaaaa ctggaaatat atttttcaaa gattatattt ctaaatatat
     2221 agagtttata aataatacgg taaaagttaa tggcattata aaacgtggat tagacccaca
     2281 gcaaggtttt tacataggga gaatagatgc aagttgtaaa aaaataatat cttttaagag
     2341 tgtagtttta ccaaattctg cacataggat tataaagaat agtgctaata tatactatta
     2401 ttataagtgt aatctagata aat
//
LOCUS       DBGJFF010000258         2416 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.263, whole genome shotgun sequence.
ACCESSION   DBGJFF010000258 DBGJFF010000000
VERSION     DBGJFF010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.263"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(82..2271)
                     /locus_tag="ACOIAF_003834"
     CDS             complement(82..2271)
                     /locus_tag="ACOIAF_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HGZ2670809.1"
                     /translation="MYEVNLERAMNYWMDLLDDYTNIAKIPNDFFYKDCSDEVVDEVF
                     KMNIEKHERLIQYSKVNNISIDSIIETIYGLILQKYIYEDDVVFGKTMNIIPIRVRVR
                     QSEKFIDVVRKLKRQWSRSLEYGYYLLSDIENKNHLNTKLINTIFIAKSFDLSYDYDI
                     EKKGYDLSVIVYTKRALKIKIKYKPSIYKRETIRRILNQFDYIIEQIIEDNDILIEDL
                     EFVCKEEEKVLLEDFNNNQYEIPYDKSYVDLFREQVKVTPSNIAARDENRKLTYKELD
                     EVTDKLAGYLNHIGVESEDIVAVMLPRDINIIITAIGIMKSGGAFFPIDTSNPEERLN
                     YLLEDSNAKVVITTDELKSKVVNENTIVLDINDEEMFKLGYELTEKITSSNCAYTIST
                     SGSTGRPKTIAIEHKSLVNMCYYSVKSISATENDICGIYLSFSFDAVMKQLFPYLLVG
                     ASIDIMPEEAKFDEYTVNEYCEENDITILALPTAFARLFIQNCNNNSLRVVQTGGERL
                     KGYRKRNYELYNEYGPTEFTVVSTSFHVDREYGKIPIGKPIFNTYAYVLDKKNKLCPI
                     GAPGELCLSGIQISRGYLNKKGLTEQVFVENPYKTCEHNKLMYRTGDLVRWLDDGNLD
                     YIGRMDNQVKIDEFRIELYEIENIINNITEIKSVVCISRTNDDGDMYICAYYVIDEED
                     SGKINERTIREYLNEHLPPYMIPTIIMRIDKIPVTPIGKVNKRALPE"
BASE COUNT          756 a          436 c          233 g          991 t
ORIGIN      
        1 atgcaaattg attcttttat aaaaataagt gtcataaaga aatgtctgaa atcactttat
       61 gacacctatc ataataaaaa attattctgg taatgctctt ttatttactt ttccgattgg
      121 tgtaactggt atcttatcta tcctcattat tattgttgga atcatatatg gaggtagatg
      181 ctcatttaaa tattctctaa tagttctttc atttatctta ccactatctt cctcatcaat
      241 tacataatat gcacatatat acatatctcc atcatcattt gttctcgata tacaaacaac
      301 acttttaatt tctgtaatat tatttattat attttcaatc tcatatagct ctatcctaaa
      361 ctcatcaatt tttacttgat tgtccatcct tccaatataa tctaaatttc catcatctaa
      421 ccatcttact aaatcaccag ttctatacat taacttatta tgttcacaag ttttgtaagg
      481 attctctaca aatacttgct ctgttagccc ttttttattt aaatatcctc ttgatatttg
      541 aatgcctgat agacatagct ctcctggtgc tccaattgga catagtttgt tctttttatc
      601 taatacatat gcataagtat taaatatagg ttttcctatt ggtatttttc catattctct
      661 gtccacatga aaagaagtcg atacaacagt aaattcggtt ggtccatact cattatatag
      721 ttcataatta cgctttctat atccttttaa tctttctcct ccggtctgta caactctaag
      781 tgaattatta ttgcaatttt gtataaataa ccttgcaaat gctgttggca atgctagtat
      841 agttatgtca ttttcctcac agtattcatt tactgtatat tcatcaaatt tagcttcttc
      901 aggcataata tctattgatg cacctacaag caaataaggg aatagttgtt tcattacagc
      961 atcaaaactg aaacttaagt atataccaca tatatcattt tctgtagcgc ttatagattt
     1021 tacgctataa taacacatat ttactaaact cttatgttct atagctatgg tctttggcct
     1081 accagttgac cctgaagtag atatcgtata tgcacaattt gatgatgtta ttttttcagt
     1141 taattcatat ccaagcttaa acatttcttc atcatttata tcgagtacta tggtattctc
     1201 atttacaacc ttacttttga gttcatctgt agttatcact acttttgcat tactatcttc
     1261 aagaaggtaa ttcaatcttt cttctggatt acttgtgtct attggaaaaa atgctccccc
     1321 tgatttcata atccctattg ctgtaataat tatgttaata tctcttggta acattacagc
     1381 tactatgtct tcactttcca ctcctatatg atttaaataa cctgcaagtt tatcagttac
     1441 ttcatccaat tctttatatg ttaactttct attttcatcc ctagcagcta tattactagg
     1501 tgttacctta acttgctccc taaataaatc aacataactt ttatcatagg gtatttcata
     1561 ttgattgtta ttaaagtctt ccaataatac tttttcttct tccttacata caaattctaa
     1621 atcttctatt aaaatatcat tatcttctat tatttgctct attatataat caaattgatt
     1681 taaaattctt ctaatagttt ctcttttata tatagatggt ttatacttta tttttatttt
     1741 aagtgccctc ttagtatata ctattacaga taaatcatat cctttttttt ctatgtcata
     1801 atcatatgat aaatcaaaac ttttagctat aaatattgtg tttataagct tagtatttag
     1861 atgattttta ttctctatat ctgataacaa atagtatcca tactctaaag acctgctcca
     1921 ctgccttttt agcttcctta caacatctat aaatttttca ctttgtctaa ctcttactct
     1981 tattggaatt atattcattg ttttaccaaa aactacatca tcttcatata tatacttttg
     2041 aagtataagg ccatatatag tttctatgat agagtctatg ctaatattgt tcactttaga
     2101 atattgaatt aacctttcat gtttctcaat attcatctta aaaacctcat ctactacttc
     2161 atcagaacaa tctttataaa aaaaatcatt tggaattttt gctatattcg tataatcatc
     2221 taacaaatcc atccaataat tcatagctcg ttctaaatta acttcataca tatcaaaatt
     2281 cccccttact actaagtcta tagaaaaaat tcttttggaa ttttattttc acaatatata
     2341 aatatatgaa taatacatat gcaagctcta aaaacatgag cttgcataaa attacactta
     2401 ttatgactac taaatt
//
LOCUS       DBGJFF010000259         2392 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.264, whole genome shotgun sequence.
ACCESSION   DBGJFF010000259 DBGJFF010000000
VERSION     DBGJFF010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.264"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            <1..684
                     /locus_tag="ACOIAF_003835"
     CDS             <1..684
                     /locus_tag="ACOIAF_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HGZ2670810.1"
                     /translation="GRILNATISKEPSGRYYVSLCCTDVDIGAFENTNNHIGLDLGIK
                     EFCISSCGDFIENPKYLKKSLNKLAKLQRELSRKTIGSLNRNKARLKVARFQEHIANQ
                     RKDFLQKLSTKLIKENDIICIEDLQVKNMIRNRKLSRLISDVSWSEFIRQLEYKANWH
                     GRQIVKVGKFFASSQICNKCGYKNEEVKDLNIREWICPSCNETHDRDINASINILKEG
                     LRLITIQNK"
     gene            808..2322
                     /gene="ade"
                     /locus_tag="ACOIAF_003836"
                     /pseudo
     CDS             808..2322
                     /gene="ade"
                     /locus_tag="ACOIAF_003836"
                     /inference="COORDINATES: protein motif:HMM:TIGR01178.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
BASE COUNT          937 a          255 c          459 g          741 t
ORIGIN      
        1 ggtagaatac ttaatgctac tatatctaaa gaaccaagtg gtagatatta tgtatcatta
       61 tgctgtactg atgtagatat tggagcattt gaaaatacta ataatcatat aggtttagat
      121 ttaggaatta aagaattttg tatctcaagt tgtggagact ttatagaaaa tcctaagtat
      181 cttaagaagt cattaaacaa acttgctaaa ttacaaagag aattatcgag aaaaacaatc
      241 ggtagtttaa atagaaataa agcaagatta aaagttgcaa gatttcaaga acatatagct
      301 aatcaaagaa aggattttct acaaaaatta tctactaaat taataaaaga gaatgatatt
      361 atttgcatag aagatttaca agtaaagaat atgattagaa atcgtaaact atctcgctta
      421 atttctgatg tgtcatggtc agagtttatt cgtcaattag aatacaaggc taattggcat
      481 ggtagacaaa ttgtaaaggt aggtaaattc tttgcaagtt cacaaatatg caataaatgt
      541 gggtataaaa acgaggaagt gaaggactta aatataagag aatggatttg tcctagttgt
      601 aatgaaactc acgatagaga tataaatgca agtataaata tattaaaaga aggattaaga
      661 ctaataacaa ttcaaaataa ataacttatg taagaaccat aggaactacg gggatagcct
      721 gtggagaatt agtaagacat attttagtat gcaaaattct gtgaagcagg aaccctgtga
      781 ctttagtcat gggaggttca gtgtagcaat aaatatatat cacctggctt tattgatgca
      841 catatgcata ttgaatctac aatggtaatg ccaattgaat tatcgaaaaa attattaaaa
      901 tcaggtacga caactatcat agccgaccca catgagttgg tcaatgtaaa aggagttagt
      961 gcaatagatt ttttattaga atcgacaaaa gatataccac ttaatgtata tattatggtg
     1021 ccttcatccg tacctgcaac aagttttgaa actaatggag ttggtaaatt tagtgcaaaa
     1081 gacatggaga gttatgtaaa taatcctaga attttaggtt taggagaggt aatgtgtttt
     1141 aatgatgtta taaattctga aaatgaaatc ttggataaac tagagctttt taaaaataaa
     1201 gttgtagatg gacatgcacc taatataaat ggtaaatcac ttcagacata tgtatgtgca
     1261 ggaatagaaa atgaccatga atgtataact tttgatgaag tatatgaaaa attgagagca
     1321 ggattaaaaa ttttaataag agaaggaagc acagcaaaaa atttaaagtc tatagtaagt
     1381 ggtatgctaa agcataactt gccaattgaa gaatttatgt tttgcacaga tgataagcac
     1441 ttagatgata tagaaaaaca aggtcacata agatggaata taaaatgtgc gattgattta
     1501 ggtatggaac ctgttagggc tataaaagtt gcaacttata attctgcaaa agcatatggg
     1561 ttaaggaaaa ttggagcaat aggtgcgggt tataaggcag atattgttgt attgaatgat
     1621 ttagacaaaa tggaagtgga tagtgtatat aaagatggta atttggtaaa cgaagagatg
     1681 ttttctaatt acaattatga gattaaagat aaagaattgc ttaatacagt caagtttaaa
     1741 tatattaata aagaaaaaat acagttaaaa gtaagtgaaa aaaattatgt aatggagatt
     1801 gtaccttatc aaattttgac aaataaggta tatgaatctt taccatgtgc agatggatat
     1861 tttgttccaa ataaagagta ttcaaaactt tgtgttgttg agagacatag aatgactgga
     1921 aatgtagcag ttgctccact aaaaggattt ggcataaaaa atggagcaat tgctactact
     1981 gtggcccatg attctcataa tattatagtt gcaggggata atgatgatga tattctagta
     2041 gctataaatt acttaaagga aatacaaggt ggttatgtaa ttgtatcaaa tggaaaagtt
     2101 cttgcacacc taagtcttca agtagctgga ttaattagta cttttacagc tgaagaagta
     2161 caagaaatta ctgataatat gttggagata gcaagaaaaa tgggagttcc agagtatgta
     2221 gaccctttta ttacactttc ttttatggca cttccagttg ttccacaaat aagacttaca
     2281 gatttaggtc tatttgatgt tgaggaattt aaatttatat agataatata aaagtaataa
     2341 taaatgaggc tttctcaaaa tagggataag tctcttttta ttgttgaaat ga
//
LOCUS       DBGJFF010000260         2318 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.265, whole genome shotgun sequence.
ACCESSION   DBGJFF010000260 DBGJFF010000000
VERSION     DBGJFF010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.265"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            197..1414
                     /locus_tag="ACOIAF_003837"
     CDS             197..1414
                     /locus_tag="ACOIAF_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HGZ2670811.1"
                     /translation="MLGKKCMDYLQTLGKISSTTNGLTRLILTQEHKKSIDLISSWME
                     GLNLDIEIDDIGNVIGTYKSSFPNAPTLVVASHQDSVKCGGIFDGMLGIIVPLVGLEE
                     AKHNNRSYPFNIKLIAFAEEEGTRFETSLMGSKVFAGTFKEELLKSVDENGITLEEAV
                     TKFGFNTKNLTNLHPRKDVDAYLEFHIEQGPVLENESLPAGIVSSITGFKSFKISVNG
                     KSGHAGTLPMNMRLDAGCCACECVLAIEKVAKTTTDLVATVGKMNFYPSSSNVVPERA
                     EFTLDVRSCSQEILDNSVEKIFNEISHICENRKLNYTSELAFENVPVPCSNKITKIIE
                     KSFIDLNLNPFYIYSGAGHDAQEMDNITDIGMVFIRCAGGVSHNPNESVSVDDLDTAV
                     KIFLKILDNLDLK"
     gene            1576..2274
                     /locus_tag="ACOIAF_003838"
     CDS             1576..2274
                     /locus_tag="ACOIAF_003838"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047661 - amino-acid racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HGZ2670812.1"
                     /translation="MNEKLIGILAGMGPRSTAPFVDLIIDECQSQYGAKYDDEFPKMM
                     IYSLPTPFYIDRPINHDLMKETIIDGLQKLESTGVNFIAMPCNSAHIYFKELKESINI
                     PILNIVEETVKKLPTISQKVTLFSTSSTFESTIYQNGIIHNGHEFIFKDEWQIKLNNL
                     IQNIKMDKENPHNIDIWNKLIEDVKNESIENIVIACTDLNVVLEKSYPSINIVDSSKC
                     LAESVVNKYLKLVK"
BASE COUNT          888 a          270 c          346 g          814 t
ORIGIN      
        1 ctataataat aaaaatcata tttcatattt ttaacttaaa acttctgctc ttcgtctaca
       61 gactttatgt ttttatatca ttattaatac taaaatagta ttttactata catacaaaat
      121 tatttgaaag ttcaaaaatt ataagtataa aagctaatct tagcttaaat aaaaattata
      181 tttttaggag gtaaatatgt taggaaaaaa atgtatggat tatctacaaa cattaggaaa
      241 aataagttca acaacaaatg gtcttacaag acttatcttg actcaagaac ataaaaaatc
      301 tattgattta attagttcat ggatggaagg tttaaatctt gatattgaaa ttgatgacat
      361 tggaaacgtt ataggtacat ataaatcaag ctttccaaat gctcccacat tagtagttgc
      421 atctcatcaa gatagcgtaa agtgtggagg tatatttgat ggtatgttag gtataatagt
      481 gcctctagtt ggtcttgaag aagcaaaaca caataatagg tcttatcctt tcaatattaa
      541 gctaattgct ttcgctgaag aggaaggaac tagatttgaa acttctttaa tgggaagtaa
      601 agtttttgct ggtactttta aagaggaact tcttaaatct gttgatgaaa atggaattac
      661 acttgaagaa gctgtaacaa agtttggatt taatacaaaa aacttaacaa atttgcatcc
      721 tagaaaagat gtagatgcat atttagaatt tcatatcgag cagggtcctg ttttagaaaa
      781 tgaatctctt cctgctggaa ttgtatcaag tattacagga tttaaaagtt ttaagatatc
      841 agtaaatggt aagtctggac atgctggtac acttccaatg aatatgagac ttgatgctgg
      901 atgttgtgcc tgtgaatgtg tattagctat cgaaaaagta gctaaaacga ctactgattt
      961 ggttgctact gtaggaaaga tgaatttcta tccatcttca tcaaatgtgg tacctgaaag
     1021 agctgaattt actcttgatg tacgctcttg ttcacaagaa attcttgata attctgtaga
     1081 aaagatattt aatgaaatat cacatatatg tgaaaataga aaacttaatt atacaagtga
     1141 acttgctttt gaaaatgttc ctgtaccatg ttctaataaa ataacaaaaa tcatagaaaa
     1201 aagttttata gatttaaatc ttaatccttt ttatatttat agtggtgctg gccatgatgc
     1261 tcaggaaatg gataatataa ctgatattgg tatggtattt ataagatgcg ctgggggcgt
     1321 aagtcataat cctaatgaaa gtgtttctgt agatgacttg gatactgctg taaaaatatt
     1381 tttaaaaata ctagataatc ttgatttaaa ataattctta tataagaaag tatattaaaa
     1441 tatataatct aaaaaatcac tttatattta tattatataa gtgtacaata taatctatta
     1501 taaaatataa ttttatgctt ttatagattt ttattttata cataaatata tttatatata
     1561 aaggagataa tttttatgaa tgaaaaatta attggtattt tggctggaat gggtccaagg
     1621 tcaacagccc cttttgttga tttaattatt gatgagtgtc aatctcaata tggagcaaaa
     1681 tatgatgatg aatttccaaa gatgatgata tattcattac ctaccccatt ttatattgac
     1741 cgtccaataa atcatgacct tatgaaagaa actataattg atggattaca aaagcttgaa
     1801 tcaactggtg taaattttat tgcaatgcca tgcaattcag cacatatata ctttaaagaa
     1861 ttaaaagagt ctattaatat accaatatta aatattgtag aagaaacagt taaaaaatta
     1921 cctactatct cacaaaaagt aactcttttt tcgactagtt caacttttga gtctacaatt
     1981 taccagaatg gtataataca taatggacat gaatttatat ttaaagatga atggcaaata
     2041 aaattaaaca atcttattca aaatataaaa atggataaag aaaatccaca caatattgat
     2101 atttggaata aacttattga agatgttaaa aatgaatcta tagaaaatat agtaattgct
     2161 tgtactgatt taaatgtagt tttagaaaaa tcatatcctt caataaatat tgttgattca
     2221 tcaaaatgct tggctgaatc agttgtcaat aaatatctaa aattagttaa ataaattttt
     2281 catgcactat attataaaaa atatatgtta taagttta
//
LOCUS       DBGJFF010000261         2302 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.266, whole genome shotgun sequence.
ACCESSION   DBGJFF010000261 DBGJFF010000000
VERSION     DBGJFF010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.266"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(144..1436)
                     /gene="brnQ"
                     /locus_tag="ACOIAF_003839"
     CDS             complement(144..1436)
                     /gene="brnQ"
                     /locus_tag="ACOIAF_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HGZ2670813.1"
                     /translation="MGEKQINFKDVIVIGFALFAMFFGAGNLIFPPYLGVLSGSSWLV
                     AFIGFLFADGGLALLAVIAATKFNGDTSKMFSRAGKGLSIALGCAMVICIGPLLAIPR
                     TAATTYEMGILPTIGSGISPVIFSIVFFALVLVLTIRPSKVVDIVGSILTPALLIALA
                     VLIVKGIVSPLGEIRDSSLIQNVFAEGITQGYQTMDALAASVFASIIIMSVIAKGYTG
                     EKEKMKATISAGVIAVIGMALVYGGLCYLGATVSQQYGQDVQQTALIVAITAALLGTT
                     GKILLAIIVALACLTTAIGLSSAAGQYFSTLTDGKLKYEHIVIVVCVFSAIISNFGVS
                     TIIQFSSPILSMVYPATITLVILALFSNKIKNDNVFKCAAYMALLVSVLTVATSFGVK
                     IPLVNSLPFASLGFNWIVPVVIAGIIGNFIPSKSSKTA"
BASE COUNT          771 a          378 c          247 g          906 t
ORIGIN      
        1 cttcaaaaat aaatttttaa atttatcaaa caggattaat aatgttcttt tatctacaat
       61 aatttaataa ttcttttaaa tataaaaagg aattgcctat ataatttaaa ggcaattcca
      121 taattatcaa ttagttttta ttattatgca gttttactgc ttttagatgg aataaagttt
      181 cctattattc ctgctattac aactggaact atccagttaa atcctaatga agcaaatggt
      241 aagctattaa ctagaggaat ttttacacca aaacttgttg caactgttaa tacacttact
      301 aataatgcca tgtatgcagc acatttgaac acgttgtcat ttttaatttt attactaaat
      361 aatgctagta taactagagt tatagttgct ggatatacca tacttagtat aggagaagag
      421 aattgtatta ttgtacttac tccaaagttt gatatgattg cactaaatac acaaactact
      481 ataactatat gctcatactt taattttcca tctgtaagtg ttgagaaata ttgaccagct
      541 gctgaagaaa gacctatagc tgttgttaaa catgctaatg caactataat agccaataaa
      601 attttacctg tagttccaag taaagctgct gttattgcaa ctattaaagc tgtttgttga
      661 acatcttgtc catattgttg agacacagtt gctccaagat aacataatcc accataaact
      721 aatgccattc ctattactgc aattacacct gcacttattg tggctttcat cttttctttc
      781 tcacctgtat aacctttagc tattactgac atgattataa ttgatgcaaa tacactagct
      841 gccaatgcat ccattgtttg ataaccttga gttatacctt ctgcaaatac attttgtatt
      901 aaagaactgt ctcttatttc tcctaatgga gatacgatac ctttaactat taatactgct
      961 aaagctataa gtaaagctgg agttaatatt gacccaacta tatctacaac ttttgatggt
     1021 cttattgtta aaaccaatac tagagcaaag aatactatag agaatattac tgggcttatt
     1081 ccagaaccta ttgtaggtaa tatacccatt tcataagttg ttgctgctgt tctaggtatt
     1141 gctaaaagtg gccctataca gattaccatt gcacatccta atgcaatact taatcctttg
     1201 cctgctcttg agaacatctt cgatgtgtct ccattgaact tagttgctgc tataactgct
     1261 aataatgcca aaccaccatc tgcaaaaagg aatcctatga aagctactag ccaactactt
     1321 ccagataaaa ctcctagata tggagggaag attaagttcc ctgcaccaaa aaacattgca
     1381 aatagtgcaa atccaattac aattacatct ttaaaattga tttgtttctc acccatttaa
     1441 agcaccccca tttgaatttt actacctttt actaaaaaat aagtccaatc tcttaattaa
     1501 atgaaaacct tttgacgaga ttttatttaa ttatacattt ttgacttaaa tttgtcaatt
     1561 atgtttcata atttataaaa tcttcacaat atttaataca actttcttaa ttaaacaaaa
     1621 tcaacctgta tactttaaac atagttggtt ttttgttttt tctctaattt ttcttgataa
     1681 aattaactat tccgaataat taaaatatta agacttttta gattattcga tttattatta
     1741 agttgtattg ctttatatac tgtatatatc actctccttt tttacattca tataatcttt
     1801 ttaattcaat ttaaatttta aatattttgt gtttattata taatctgaaa aaaaagcagt
     1861 caatagcatt tttaattttt ttaattttca aaattatgat tcttaaaaaa tcgttttcct
     1921 tttatcataa cacctattta cgttaattct tgtagtattt tgtatttatt ttgctaaatt
     1981 gacagttaac tttattcaat ttttattcta cttacgtaga tttacctctt gttttttact
     2041 cttaaaacca ttttttctga aaattcctta ttatgattta tcaacttatt tttcttcata
     2101 acaattactc tctaacttcc aacttactta aactttgcag ttgctagtat tcacgtttac
     2161 atacttatat gtcataatta actaaaaatt cttaatatat ctctcaatga aaacaattta
     2221 ctcatttatt taatacattt atataatcat atgtcttaaa ttttgtttta ttattttatt
     2281 attgtcacaa ttttatttat at
//
LOCUS       DBGJFF010000262         2281 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.267, whole genome shotgun sequence.
ACCESSION   DBGJFF010000262 DBGJFF010000000
VERSION     DBGJFF010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.267"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(56..319)
                     /locus_tag="ACOIAF_003840"
     CDS             complement(56..319)
                     /locus_tag="ACOIAF_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670814.1"
                     /translation="MIKNTKQITVEVNEILLQEMSKITNDENNILTDLINESIVNYIL
                     ENKKHNIENKDLEEVIKFYKKENTEKPKSAARAIAEAKTHTLY"
     gene            635..1210
                     /locus_tag="ACOIAF_003841"
     CDS             635..1210
                     /locus_tag="ACOIAF_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670815.1"
                     /translation="MKLKEALITCVVCSVCLTPLLVNGETKIDADHCKESIVNIDTIV
                     KSGKSFNSKYNQGMEDYKNSDEYKLKVQKEKLQNQLGIKIKKLVPITFEVSYYTSLNQ
                     ENGYGAITATGDDLEDGFVANNHLSFGTKIIVDGKLKTVMDRGSSKYFSNSNAIDVFV
                     PKWSNESESEYYDRVNNMGRHYKKGYIIVEE"
     gene            1308..>2281
                     /locus_tag="ACOIAF_003842"
     CDS             1308..>2281
                     /locus_tag="ACOIAF_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4003 domain-containing protein"
                     /protein_id="HGZ2670816.1"
                     /translation="MEQSFINGKVNLLIENYKALNEVKGSWQMGLIQHSCALAFTLKN
                     KRISPRLVEERIELIKKNTGLFSNFRGYNMFYMATLLSFESNPESSFKMILDIYKELK
                     SEKFWGDTYLPLTASIVYENREKMDYLTCISKMKIIYDYMRKKHPFLTSSDDYCNIAL
                     IAIHSKNLDEDLEYIEKCYEFLNENGFYKGNDLQALSQILLFSDDRTMLKCKKTIELK
                     KAFKENDCKMNYYGYPIIGAISLLDYREDEIIEEIKNVSNKLKEEKGFGNWSLGKSNR
                     VMISSAIVASIYADLIRNENNIGSITNNIFLNIVVAIQIACVMAATSAA"
BASE COUNT          917 a          207 c          360 g          797 t
ORIGIN      
        1 attaataatt attaatataa aaaagttcct agtttttagg aactttttta tttgattaat
       61 acagagtatg agtttttgct tcagctatag ctcttgctgc acttttaggt ttttcagtat
      121 tttctttttt gtaaaattta atcacttctt ctaaatcttt attttcaata ttatgttttt
      181 tgttttcaag aatataattt acaattgatt catttatcaa atctgtcaaa atattatttt
      241 cgtcattagt tatcttagac atttcttgta ataaaatttc attaacttct actgtaattt
      301 gttttgtatt ttttatcata tcaaccacct cttaagaaaa ttatatcatc tcaaaaaaat
      361 aaattcaata tgttttaaaa ttatagaatt ttaattctta aattctaaaa aagagaaaat
      421 tagataaagt agttttagat tttaaaagat taataaaaaa tatttaaaga taaaagcttt
      481 attctatatg attctaagat agaggattta taagtgttta aggtgaatta aatataagac
      541 aacttcttaa agcaaatggg tgtggtattt aaatctaaat gtagacatat aatattaaat
      601 atgctaatat atcaaaataa taagggggga atttatgaag cttaaagaag cattgattac
      661 atgtgttgta tgtagtgtat gtttaactcc attattggta aatggagaaa caaaaataga
      721 tgcagatcat tgtaaagaat ctatagtaaa tatagatact atagtaaagt caggtaaaag
      781 ctttaattcc aagtataatc aaggtatgga agattataaa aatagtgatg agtataagtt
      841 gaaagtacaa aaggagaaac tacaaaatca gttaggtatt aaaataaaga agttagtacc
      901 tataactttt gaagttagtt attacacaag tcttaatcaa gaaaatggat atggagcgat
      961 aactgctaca ggagatgatt tagaagatgg ttttgtagca aacaatcact taagttttgg
     1021 aactaagatt attgtagatg gaaaattaaa aactgttatg gatagggggt ctagtaagta
     1081 ttttagtaat tctaatgcaa tagatgtatt tgtgcccaaa tggagtaatg aaagtgaatc
     1141 agagtactat gatagagtca ataatatggg aagacattac aaaaaaggct acattattgt
     1201 tgaagaatga tgttctttga agtaatatgt attgattgta gtatttaata tatatataat
     1261 aaatataaaa ctattagtac aatgagatat attactagga gggcaatatg gaacaatcgt
     1321 ttataaatgg aaaagtaaac ttattaatag aaaattacaa agcattaaat gaagttaaag
     1381 gttcttggca aatgggattg atacaacata gttgtgcact tgcatttacc cttaagaaca
     1441 agagaataag tcctagatta gtggaagaac gaatagagct tattaaaaaa aatactggat
     1501 tattctctaa ttttagagga tataatatgt tttatatggc aacactactt tcgtttgaat
     1561 caaatccaga aagtagtttt aagatgattt tagatatata caaagaactt aaaagtgaaa
     1621 agttttgggg cgatacatat ttaccactta cagcatcaat agtttatgaa aatagagaga
     1681 aaatggatta tcttacatgt atatcaaaaa tgaaaataat atatgattat atgaggaaaa
     1741 aacatccatt tctaacttct agtgatgact attgtaatat agcacttatt gcaattcatt
     1801 caaaaaattt agatgaagat ttagaataca tagaaaaatg ttatgaattt ctaaatgaaa
     1861 atggttttta taaaggaaat gatttacaag cattatccca aattctttta tttagtgatg
     1921 atagaactat gcttaaatgt aaaaagacta ttgaacttaa aaaagcattt aaagaaaatg
     1981 attgtaaaat gaattattat ggatatccaa taataggagc aatttcatta ttagattata
     2041 gagaagatga aataatagaa gagataaaaa atgtttcaaa taaacttaaa gaagaaaaag
     2101 gctttggaaa ttggtcttta ggaaaaagta atagagtaat gattagttct gctattgtag
     2161 cttcaattta tgcagatttg atacgaaatg agaataatat aggttctata actaataata
     2221 tatttttaaa tattgttgtt gctattcaaa ttgcatgtgt aatggcagca acaagtgcag
     2281 c
//
LOCUS       DBGJFF010000263         2266 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.268, whole genome shotgun sequence.
ACCESSION   DBGJFF010000263 DBGJFF010000000
VERSION     DBGJFF010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.268"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            540..1982
                     /locus_tag="ACOIAF_003843"
     CDS             540..1982
                     /locus_tag="ACOIAF_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HGZ2670817.1"
                     /translation="MRTIRRVLALGLTLAIFLINVPNVDALTSDTIKGNNIYETAGLI
                     ADKKSYDTAIMVNMDNSIADGLSASGLAGAVDAPILLAQKNKIPNETKQRLKNVKKIY
                     IIGKELSISKSVETELKNTGAQVTRLGGDDRIKTSYSVAKEVNGIKKVDEVILTNAYK
                     GEADTISAAPVSVRDIAPIVLTDGKSVPFSTSNVKTYAVGGSISMSTSLVNKTNAKRL
                     GGSDRYDTNKKVIKEFYPDASEFYLSDGYDLVNALTGSTIAKENPIVLVSESSDKSIL
                     AGADKITRLGSISDSVYNKCVSAAQNNGDSSTKGESPMKNETSILGQPTASLEACLKW
                     AKSKKANDLFIELIPILYDTAVQEGVNPVLAVAQSAKETGFCNFGGVLDASFKNPCGL
                     KTSVGGSDTDKNAHSRFDTWEEGILAQIQHLCLYAGQDGYPLSNPVDPRHEKSLFGKA
                     KTVESLSNNWAGGQYGQDLVRMMGEIEATK"
     gene            2024..>2266
                     /locus_tag="ACOIAF_003844"
     CDS             2024..>2266
                     /locus_tag="ACOIAF_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670818.1"
                     /translation="MKKSNIFSIIFPTVIMVLMFLTFATDIFNIPDIQSKSIFVVGMV
                     LIFPISFLIQGIICVLNKTNWILSLIVSLVTYIVLMY"
BASE COUNT          882 a          239 c          398 g          747 t
ORIGIN      
        1 aaaatattag aattaaatta aaagtgaaaa gtagattgat atataaatag taaatactta
       61 attaaaaaaa gataatgata gcaataatta ttgttaccaa gaatctacta cttcaagtgt
      121 taccttaggt atttaagttg atagtggttt gcacgtaata aacttgatgt aatatatatt
      181 cttaataata ttgataaaag atttacttaa aaataatata gatcatgtaa acatataatc
      241 tatttattgt atacaataaa atttcatttt gtaaaacagt gaattaaata tgtaatatag
      301 atatcaattt ttattacaaa ttgttactaa atataggtac attagaacaa aaatctagta
      361 aattgtaact aaaatgtaaa tttttccttt aatctataca atattatgga aactttttgt
      421 aatttatgat ataataacaa atatataatt attaaattat aataaatgac tatagcgtag
      481 atgtaatact atgttatagt catttttgaa ttaaacttga tatatcaagg gggaagaata
      541 tgagaacaat tagaagagtt ttagcattag gtttaacatt agctatattt ctaataaatg
      601 tacctaatgt agatgcatta acatcagata caattaaagg taacaatata tatgagacgg
      661 ctggattaat agcagataaa aagagttatg atacagcaat tatggtaaat atggataatt
      721 ctatagcaga tggtctttca gcaagtggtc ttgcaggtgc tgttgatgct ccaattttgc
      781 ttgcacaaaa aaataagata ccaaatgaaa caaaacaaag attaaaaaat gttaaaaaga
      841 tatacataat aggtaaagaa ttatcaataa gtaagtcagt agaaactgaa ctaaaaaaca
      901 caggggcaca ggtaactaga ttaggtggag atgacagaat aaagactagt tatagtgttg
      961 ctaaagaggt aaatggtatc aaaaaagttg atgaagtgat attaactaat gcatataaag
     1021 gagaagcaga tacaataagt gctgcacctg tatcagtaag agatatagct cctatagtac
     1081 ttacagatgg aaagagtgtg cctttttcaa caagcaatgt aaaaacatat gcagtaggtg
     1141 ggagtatatc aatgagcact agtttagtta ataaaactaa tgctaaaaga cttggtggtt
     1201 cagatagata tgatacaaat aagaaagtta taaaagagtt ttatccagat gcatcagaat
     1261 tttatttgag tgatggatat gatttagtaa atgcacttac aggttctaca attgctaagg
     1321 aaaatccaat tgtattggtg tcagaaagta gtgataagtc tatattagca ggagcagata
     1381 agattactag attaggttca ataagtgaca gtgtgtataa taagtgtgtt tctgctgcac
     1441 aaaataatgg cgattcgtct acaaaaggtg agtcacctat gaaaaatgag acaagcatat
     1501 taggtcagcc aacagctagt ttagaagcgt gcttaaaatg ggcaaaatct aaaaaagcta
     1561 atgatttatt tatagagtta ataccaatat tatatgatac tgctgttcag gaaggtgtta
     1621 atcctgtttt ggcagtagct caatctgcaa aagaaactgg tttttgtaat tttggtggag
     1681 tattagatgc atcatttaag aatccttgtg gacttaaaac ttctgtaggt ggttctgata
     1741 ctgataagaa tgcccattca agatttgata cttgggaaga aggaatatta gctcaaattc
     1801 aacatttatg cttatatgca ggacaagatg gttacccact ttcaaatcca gtagacccta
     1861 gacatgaaaa atctttattt ggtaaggcta aaacagttga aagtttatct aataattggg
     1921 ctggaggtca atatggacaa gatttagtaa gaatgatggg agaaattgag gcaacaaaat
     1981 aattgaagat tatcaaatat ccaataaata aggattgatt attatgaaaa agagtaatat
     2041 ttttagtatc atatttccta cagtcataat ggtgctaatg tttttaactt ttgctacaga
     2101 tatatttaat attccagata ttcaatctaa aagtattttt gttgtgggaa tggttttgat
     2161 ttttccaatt tcttttttga tacaagggat aatttgtgta ttaaataaga ctaattggat
     2221 tttatctctt attgtgtctt tagtaactta tatagtatta atgtat
//
LOCUS       DBGJFF010000264         2178 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.269, whole genome shotgun sequence.
ACCESSION   DBGJFF010000264 DBGJFF010000000
VERSION     DBGJFF010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.269"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            91..2115
                     /locus_tag="ACOIAF_003845"
     CDS             91..2115
                     /locus_tag="ACOIAF_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HGZ2670819.1"
                     /translation="MDKKKLILITYDKLNSDHYKEELTNFFGDEIIIETQNILDGIKE
                     NLEGDVVLSLSPLTSNFLIKHFKEDIEIIHGTKALSKLGYEKMMKLPPGTKSLLMTTN
                     KTSAFEMATYLYKIGINHIDFVPTYPDCDEIYDLDTAITPGQIRFIPKYIKNIVDLGW
                     RKISLDTYMSLLVVLKLKNEKFIEKLYKLSKETLSHDFLNTSLDNISKLKTILYMTID
                     EIGDGLIFFNTFNKVTFVNKSLLNMLELDEKLIKSPSLMEYMPKSFLDKITKNLNIDN
                     MIIYIDEIDKKFILSKKPFYLYKNIEGCLITLKDVNNIEILEQKIRSDSVKRGYVAKY
                     KFNNIIGNSSIIKDCIKKAKKMALTDNPILITGETGTGKEAFTQSIYNHSNRKNKPFV
                     AINCASLPSELLESELFGYEDGSFTGAKKGGKKGLFELAHTGTIFLDEIGDMPHDLQV
                     KLLRVLQEKEIRKIGGTSIIPIDVRILAATNKDLEKLIIENKFRMDLFYRISMFTLDL
                     PPLRKRLEDIPLLLESFLKELPYKNIKLDKSLLEALNSYTWMGNIRELRNCVEYMAYM
                     GSNYLTINDLPQNISSKLNNNHMSSNMSIFNDLNQYDKNICISILKSLHMKPMGRTKL
                     MKFMEYNVTEYEVRNMLEYLTRNGYLISSKGRKGSSITEKGIKIIENNII"
BASE COUNT          921 a          250 c          301 g          706 t
ORIGIN      
        1 taaatttata ctactatttg ttgtataata taacttgcct tatactttaa atactttaaa
       61 aataaatact attttgttag gagaaataaa atggataaaa agaagttaat ccttataaca
      121 tatgataaat tgaatagtga ccactacaaa gaagaattaa caaatttttt tggagacgaa
      181 ataatcatag aaacacaaaa tatacttgat ggaataaaag aaaatttaga gggagatgta
      241 gtgctttctc tatcaccttt aactagtaat tttttaataa aacattttaa ggaagatatt
      301 gaaattattc atggaacgaa agcactttca aaacttggat atgaaaaaat gatgaaacta
      361 cctccaggta caaaatcgtt acttatgaca accaataaaa cttcagcatt tgagatggct
      421 acttatctat ataaaattgg tataaatcat atagattttg taccgacata tccagattgt
      481 gatgaaatat atgatttaga tacagctata acaccaggtc agattagatt tataccaaaa
      541 tacataaaaa atattgttga tttaggttgg agaaaaatct cattagatac atatatgtca
      601 ttattagtag tattaaaatt aaaaaatgag aaatttatag aaaaactata caaattgtcc
      661 aaggagacac taagtcatga tttcttaaat acatcattag ataatatatc taaattaaaa
      721 actatacttt acatgaccat tgatgaaata ggtgatggtc taatattttt caacaccttt
      781 aacaaggtta cttttgtaaa taagtcttta cttaatatgt tggaactaga tgaaaaactt
      841 ataaaatcac cttccttaat ggaatatatg ccaaagagtt tcttagataa aattacaaag
      901 aacttaaata tagataatat gattatatac atagacgaaa tagataaaaa attcatactt
      961 tctaaaaaac cattttatct ttataaaaat atagagggtt gcttaattac attaaaagat
     1021 gtaaacaata tagaaatatt agaacaaaaa ataagaagtg attctgtcaa aagaggttat
     1081 gtagctaaat ataaatttaa taatattatt ggaaatagtt ctattataaa agattgtatt
     1141 aaaaaggcaa aaaaaatggc tcttacagat aatcctatat taataacagg tgaaacagga
     1201 acaggtaaag aagcctttac tcagtcaata tacaatcatt caaatagaaa aaataaacct
     1261 tttgtagcaa taaattgtgc ttcattacca agtgaattac ttgaaagtga actatttgga
     1321 tatgaagatg gttcttttac tggagcaaaa aaaggtggaa aaaaaggatt attcgaatta
     1381 gctcacactg gcactatatt tttagatgag ataggagata tgccacatga tttacaggtt
     1441 aaacttttaa gagtcttaca agaaaaagaa attagaaaaa ttggtggtac aagtattatt
     1501 ccaattgatg tgagaatatt agctgccaca aataaagatt tagagaaatt aattatagaa
     1561 aataaattta ggatggattt attttatagg ataagtatgt tcactttaga tttacctcct
     1621 cttagaaaga gattagaaga tatacctctt cttttagaat catttttaaa agaacttccc
     1681 tataaaaaca taaaactcga taagagtctt ttagaagctt taaattcata cacttggatg
     1741 gggaatatta gagaactgag aaattgtgtt gaatatatgg cttacatggg ttccaattat
     1801 cttacaataa atgatttacc tcaaaacata tcttcaaaat taaataataa tcatatgtca
     1861 agtaatatgt ccatattcaa tgatttaaac caatatgaca aaaacatatg catttctatc
     1921 ctaaaatcat tgcacatgaa accaatgggg aggacaaagt taatgaaatt tatggaatat
     1981 aatgtcacag aatacgaagt aagaaatatg ctagaatatt tgacacgtaa tggatactta
     2041 atttctagta aaggaagaaa aggctcatca ataactgaga aaggcataaa aataatagaa
     2101 aataatataa tttaaaccta tagtaattca tctttacttt atcaaaattt atatataatt
     2161 ataatttttt atttttct
//
LOCUS       DBGJFF010000265         2140 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.270, whole genome shotgun sequence.
ACCESSION   DBGJFF010000265 DBGJFF010000000
VERSION     DBGJFF010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.270"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            66..971
                     /locus_tag="ACOIAF_003846"
     CDS             66..971
                     /locus_tag="ACOIAF_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HGZ2670820.1"
                     /translation="MFFKDIELNSKKELDPYFDLVDYEACEYCFSTLYMWQHVYKTGY
                     YIGEDFAVLVGEYEGDSFSILPLAKKDKLPEVVDFVLEYFSKNNKKIYLRGITTEVVE
                     FLKEKYPGRFEYIEERDLFDYIYDAESLRTLAGKKNQKKRNHINYFLKEYAGRYEAKL
                     LDKENFDECLVLMKEWESNKEENNEFDESMDDELIGIKKIFNHYDILKDKVKVFGVYV
                     DGKLEAFSIGELLNPNMALIHIEKANPDIRGLYPFINQQFLVSEFKDVEFVNREEDLG
                     IEGLRKAKLSYHPCRFVEKYSVREA"
     gene            980..>2140
                     /gene="eis"
                     /locus_tag="ACOIAF_003847"
     CDS             980..>2140
                     /gene="eis"
                     /locus_tag="ACOIAF_003847"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced intracellular survival protein Eis"
                     /protein_id="HGZ2670821.1"
                     /translation="MEIRYAKEEEIESIKDIWSYCFNDTESFMKYYFNDKYKSENTVV
                     ALDEGKIISSLQLNQYKLLLNSKVYNTSYVVGVSTLPEGRGAGYMNKVMKFTLNELYK
                     KGQLVSILMPIDYRLYRRFGYEHCYDQIEYTINTDDLKNFKSSGKMIKSNLSQIDDLI
                     QIDRTFLNEVNGNVLKDEHYYENLFKEIQSEDGFLYIHEGNEKDGYIVYFLQEDKMFV
                     RELFYKNIDALKSMLKFIYNHNTQCKIVTISTPTIDKIRFILDNPKDSDIKIKPFMMG
                     RVINVKKFIEDIDIEKDINSSFNLLIEDKFIDENNGLFKISIQNKKVSVEQLDKKGAE
                     KPQEDFDIKLDINTLTQLSFSYIDVNEAIFLNDIKDVSEETLETLNCIFSKKN"
BASE COUNT          873 a          179 c          380 g          708 t
ORIGIN      
        1 ggagtttttt atatatacaa aataaaattt ttaaaattaa gcaataaaaa aggtaggagg
       61 attttatgtt tttcaaagat attgaactaa attcaaaaaa agagttagac ccatattttg
      121 atttagtaga ttatgaagct tgtgagtact gttttagtac tttatatatg tggcaacatg
      181 tttataagac aggatattat ataggtgaag attttgccgt tttagttggg gaatacgaag
      241 gagatagctt ctcaatatta ccacttgcta aaaaagataa acttccagaa gttgtagatt
      301 ttgtccttga atatttttct aaaaataata aaaaaatata cttaagaggt attactactg
      361 aagtagtaga gtttttaaaa gaaaaatatc caggtagatt tgagtatata gaagaaagag
      421 atttatttga ctatatttat gatgcagaaa gtttaagaac tctagctggt aaaaagaatc
      481 aaaagaagag aaaccatata aactatttct taaaagagta tgcaggtaga tatgaagcta
      541 aattattaga taaagaaaac tttgatgagt gtttagtact aatgaaggaa tgggaaagta
      601 ataaggaaga aaataatgaa tttgatgaaa gtatggatga tgaattaata ggtatcaaaa
      661 agatatttaa ccattatgac atattaaaag ataaggtaaa agtgtttggt gtttatgtag
      721 atggaaaatt agaagctttt agtataggag agcttttaaa tccaaatatg gcattgattc
      781 atatagaaaa agctaatccc gatataagag gtctgtatcc atttataaat caacaatttt
      841 tggtaagtga atttaaggac gttgagtttg taaatagaga agaagattta ggtattgaag
      901 gattaagaaa agctaaactt tcttatcatc cttgcagatt tgttgaaaag tacagtgtta
      961 gggaggctta atagactata tggagattag gtatgctaaa gaagaagaga tagaaagtat
     1021 aaaagatatt tggagttatt gttttaacga tacggagagt tttatgaagt actattttaa
     1081 tgataaatat aaatctgaaa atactgtagt agctttagat gaaggaaaaa ttatttcttc
     1141 tcttcagcta aatcagtata agttattact aaatagcaag gtatataata catcatatgt
     1201 ggtaggagtt tctacacttc cagagggaag aggagctgga tatatgaaca aagtaatgaa
     1261 atttacttta aacgaattgt ataaaaaagg gcaattagtt tctatattga tgcctattga
     1321 ctatagactt tatagaagat ttggatatga gcattgttac gaccagattg aatatactat
     1381 taacacagat gatttaaaaa attttaagtc aagtggaaaa atgataaagt ctaatttatc
     1441 acaaattgat gatttgatac aaatagatag aactttttta aatgaggtta atggtaatgt
     1501 gttaaaagat gaacattatt atgaaaactt atttaaagag attcaaagtg aagatggatt
     1561 tttatacatt catgaaggaa atgaaaaaga tggatatata gtttattttc ttcaggaaga
     1621 taaaatgttt gtcagagagt tattttataa aaatatagat gcccttaaat caatgcttaa
     1681 atttatatat aatcacaata ctcaatgtaa gatagttact atatctactc ctacaataga
     1741 taagataagg tttatattag acaaccctaa agatagtgat ataaaaataa aaccttttat
     1801 gatgggaaga gttataaatg taaagaagtt tattgaagat atagatattg agaaggatat
     1861 taatagctca tttaatttat taatagaaga caagtttata gatgaaaata atggtctatt
     1921 taaaatttct atacagaata agaaggtatc tgtagagcag ttagataaaa aaggtgcaga
     1981 aaagcctcaa gaagattttg atattaaatt agatataaat accttaacac agttatcatt
     2041 ttcttatata gatgtaaatg aagctatttt cttaaatgat ataaaagatg tttcagaaga
     2101 aacactagaa acacttaatt gtattttttc taaaaaaaat
//
LOCUS       DBGJFF010000266         2089 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.271, whole genome shotgun sequence.
ACCESSION   DBGJFF010000266 DBGJFF010000000
VERSION     DBGJFF010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.271"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            <1..863
                     /locus_tag="ACOIAF_003848"
     CDS             <1..863
                     /locus_tag="ACOIAF_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGZ2670822.1"
                     /translation="FMSKDVKVKRNKKYDTFSITMELERPLLYDEVENKDDKNNNRKF
                     NIKNKIFKGIGILFVLILLGFFIWINKTYEPQKLAKEALVSNSKVEVTVNENISFTPK
                     GRKVSKGLILYPGAKVEIESYAPLARKIAESGYEVVIVKMPLNLAILGTNKAQKVMDS
                     YNNIEHWVIGGHSLGGVAASNFARDNKLIEGVVFLASYPMGDELKELGKKVISIWGSK
                     DGVVNFKSLVESKQKLPDDTTYVEIEGGNHAQFGDYGKQKGDNDAIISQEKQLNITAN
                     SIIKFLKNIS"
     gene            960..1829
                     /locus_tag="ACOIAF_003849"
     CDS             960..1829
                     /locus_tag="ACOIAF_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HGZ2670823.1"
                     /translation="MEYDMPLYRPPSEAYSLIIQVTLGCSHNKCTFCSMYKSKKFKIK
                     PLEVIKNEIDIFRRYYKHVERIFLADGDALIIPMEKLRDIILYIKEVFPECERITMYG
                     SPKSIEKKSDDELKELRKLGVKMIYLGLESGSNEVLEDIKKGFSSEQLIKVGRKVKKS
                     GIKLSATVIAGLGGTKKTHQHAVDTGKMLGAISPDYVGVLSLMVEPNTELYDLLKAGE
                     FTILEDKAVLQEIKEMIKNIDANEKIVFRSNHASNYANLKGVLPDDKQRLIDEIDYYL
                     SNQKLKREEYRRL"
BASE COUNT          873 a          202 c          365 g          649 t
ORIGIN      
        1 gatttatgag taaagatgta aaggtaaagc gtaataagaa atatgataca tttagtataa
       61 ctatggaact tgagagacct ttactttatg atgaggttga gaacaaagat gataaaaata
      121 ataatagaaa atttaacata aagaataaga tatttaaggg aataggtata ctatttgtat
      181 tgattttgct tggctttttc atatggataa ataaaacata tgaacctcaa aagctggcaa
      241 aagaagctct agtcagcaat agtaaagttg aagtaactgt aaatgaaaat atttcattta
      301 caccaaaagg aagaaaagtt tctaaaggtt taattttgta tccaggagct aaagtagaaa
      361 tagaatctta tgctccactt gcaagaaaaa tagcagaaag tggatatgag gttgtaatcg
      421 taaagatgcc acttaatcta gctatacttg gaactaataa ggcacagaaa gttatggatt
      481 cttacaacaa catagaacat tgggtaatag gaggtcattc attaggagga gttgcagcat
      541 caaattttgc acgtgataat aaattaattg aaggcgttgt atttttagct tcttatccaa
      601 tgggtgatga gttgaaagaa ttaggaaaaa aagttatatc tatatggggg agcaaagatg
      661 gagtagtcaa ttttaaaagt cttgtagaat caaaacaaaa acttcctgat gatacaactt
      721 atgttgagat tgaaggagga aatcatgcac aatttggaga ttatggaaaa caaaagggag
      781 ataatgatgc tattataagt caagaaaaac aacttaatat tactgccaat agtatcatta
      841 aatttttaaa gaatatatct taaactaaaa tttaaaaaat ataattttat aagttattat
      901 ttaaagtaaa tattaataat ataatagaat gaatatttta ttacaagggg gattttaata
      961 tggaatacga tatgccactt tacagaccac caagtgaggc ttatagtctt ataatacaag
     1021 tcacactagg atgttctcat aacaaatgta cgttttgtag tatgtacaaa tctaaaaaat
     1081 ttaaaataaa gccactagaa gtaataaaaa atgaaattga tatatttaga agatattata
     1141 agcatgtaga aagaatattc ttagcagatg gagatgcatt gataatacca atggagaaat
     1201 taagagatat tattttatat ataaaagaag tttttccaga gtgtgaaaga attactatgt
     1261 acggctctcc taaatctata gagaaaaaat ctgatgatga acttaaagaa ttaagaaaac
     1321 ttggtgttaa aatgatttat ctagggcttg aaagtggaag caatgaagta ttagaagata
     1381 ttaagaaagg ttttagtagt gaacaattaa taaaagttgg tagaaaagtt aaaaaaagtg
     1441 ggattaaatt atcagccact gtaatagcag gtcttggagg aactaaaaaa acacatcagc
     1501 atgcagttga tacaggaaaa atgttaggag caatttcacc agattatgtg ggtgtactta
     1561 gtttaatggt agaacctaat actgaacttt acgatttact taaggcagga gagtttacaa
     1621 tactagagga taaggctgtg ttacaagaaa ttaaagagat gataaagaat atagatgcta
     1681 atgaaaaaat tgtatttaga agtaatcatg catcaaatta tgctaattta aaaggtgtat
     1741 tacctgatga taaacaacgt ttaattgatg aaattgatta ctatctaagt aaccaaaagc
     1801 taaaaagaga agaatataga agactatagt ttttgatata tcaatctata ttgataggta
     1861 aatactaaga ttatttagat acaaaaaatc atgctacatt aatagttgct tactattgat
     1921 gtagcatgat ttttttatta aaaaacaaaa tggtatattc atatataata tatcaataat
     1981 aatgaaaatg tgaactagtt taatagaatt ttacctatta taataaaaac aggaatttaa
     2041 ataataaaaa gtaaagtaac tttttattaa gtaactttac tttttattt
//
LOCUS       DBGJFF010000267         2069 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.272, whole genome shotgun sequence.
ACCESSION   DBGJFF010000267 DBGJFF010000000
VERSION     DBGJFF010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.272"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(221..1201)
                     /locus_tag="ACOIAF_003850"
     CDS             complement(221..1201)
                     /locus_tag="ACOIAF_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670824.1"
                     /translation="MGDTSRFYESVKNKYPKLKDGTRVHLWPKENIIIGPIYDEEDGY
                     KKSIGNVDSLSWKQIVILAECDGKNTTDDIINILSNRYRNIHNMKEKVMEFFMFYENV
                     YLTFEDDIQTLSSVFEITGNKSYITPLYFTIEIDGDNNTNDYFSEITSLLNCMYEKGC
                     RFIEVIGEDILKNKSMREIFQYMLDHFDLIVVTKDSFTIDRSLIKELDNYRHKVIWKV
                     YSKNDDTRIKLKEDIKITSLIKRGHTVVRGDREKAIKIEDMRFKDTKERFSKYGAEWS
                     HLYISSDGSVKSYSFQKDKRIYLGNIFNNSVEEIFLEMQNKIDKLEIVYQ"
     gene            complement(1365..>2069)
                     /locus_tag="ACOIAF_003851"
     CDS             complement(1365..>2069)
                     /locus_tag="ACOIAF_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HGZ2670825.1"
                     /translation="VFDKNNKVLIDKIESLNPDVIFITGDLVDGENKNFQVALDLIDN
                     LVEKYEVYHIIGNHEQKSLIKKYKHLYKDYFKELYSKKIINMENESVRIYRGNSHLNL
                     YGLIIPLECYPYLFANNKKLELSDNFVEDNLGKVNENEYNILLAHTPFFFEKYAKWGA
                     DLVLAGHVHGGIIRIPFVGGVLSPNREFFPKYDWGKYEKDNSTMILNKGLGGSKVLIR
                     LNCKPEIVKITLKYEK"
BASE COUNT          691 a          355 c          165 g          858 t
ORIGIN      
        1 ttttacataa atattttatg aaatttaaat tttttagtca ttttgaaatt tgtaaaaagt
       61 tctctaaaaa tccacagcat cataaacagc caaaatttaa gtaaaacatc ataaaaaaat
      121 ttgtccaata attttaacaa gtaatctcac ctcattataa ttattgacac aaacttaaat
      181 aaacaatcta taaatcttct ataaatctta aactttcccc ctattgatac acaatttcta
      241 gtttgtcaat tttattttgc atttctaaga aaatttcttc aacagaattg ttaaatatat
      301 tgcctaaata gattctttta tctttttgaa atgaataaga ttttacacta ccatcagaag
      361 aaatatataa atgactccac tctgctccat attttgaaaa cctctccttt gtatctttaa
      421 acctcatatc ttctattttt attgcttttt ctctgtctcc ccttactaca gtgtgacccc
      481 tttttattaa actggttatt tttatatctt cttttaattt aatacgtgta tcatcatttt
      541 ttgaatatac tttccatatc actttatgac gataattatc taattccttt attaagcttc
      601 tatcaatagt aaaactatct ttagttacca caattaaatc aaaatggtct aacatatatt
      661 gaaatatttc tctcatactt ttatttttta aaatatcctc accaataacc tctataaatc
      721 tacagccttt ctcatacatg cagttcaata aacttgtgat ttcagaaaaa taatcatttg
      781 tattattatc tccatctatt tctatagtaa agtacaaagg agtaatgtat gatttatttc
      841 ctgttatctc aaatacactt gatagtgtct gtatatcatc ttcaaacgtt aagtatacat
      901 tctcataaaa cataaaaaat tccataactt tttctttcat attatggata tttctatatc
      961 tattggataa tatatttatt atatcgtcag tagtattttt cccatcacat tcagccagta
     1021 taacaatttg cttccaactt aagctatcca catttccgat acttttttta tatccatctt
     1081 cttcatcata aataggacca attataatgt tttcttttgg ccacagatga accctagttc
     1141 catcttttaa tttcggatat ttatttttta cagactcata aaatctactt gtatcaccca
     1201 tgattcttta ctacccccca tagtttaaat attctatgct aaaaatagca ctttattttt
     1261 attaattcaa caattattca aaattggtca ttatatatgt ttaaatggat ttaacatcaa
     1321 ccttatttat atgttattga tgtttatata tgttatataa aatcttattt ctcatacttc
     1381 aatgtaatct taactatctc tggtttacaa ttaagcctaa ttaaaacttt tgagcctcct
     1441 aagcctttat ttaaaatcat agtgctatta tctttttcat attttcccca gtcatactta
     1501 ggaaagaact ctctatttgg agataataca cctccaacaa aaggtattct tattatacct
     1561 ccatgaacat gtcctgctaa aaccaaatct gctccccatt ttgcatattt ttcaaagaaa
     1621 aatggtgtat gtgctaaaag aatattatat tcattttcat taactttacc tagattgtct
     1681 tctacaaaat tgtcacttag ttctaatttt ttgttattag caaataaata tggatagcat
     1741 tccaatggta taattaaacc atataaatta agatggctat tacctctata tattcttaca
     1801 gattcatttt ccatatttat tattttcttt gaatatagtt ccttaaaata atccttatat
     1861 agatgtttat acttttttat taaagatttt tgttcatgat ttccaattat atgatatact
     1921 tcatattttt caaccaaatt atctattaaa tctaatgcaa cttgaaaatt tttattttct
     1981 ccatctacta aatctcctgt tataaatata acatctgggt ttaatgattc aattttatct
     2041 atcaatacct tattattttt atcaaacac
//
LOCUS       DBGJFF010000268         2067 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.273, whole genome shotgun sequence.
ACCESSION   DBGJFF010000268 DBGJFF010000000
VERSION     DBGJFF010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.273"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            105..1724
                     /locus_tag="ACOIAF_003852"
     CDS             105..1724
                     /locus_tag="ACOIAF_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HGZ2670826.1"
                     /translation="MNIVISLIGGLGLFLYGMSLMGEGLQKSAGDKLKKIIELLTSNV
                     VMGVLVGTVVTGIIQSSSATTVMVVGFVNAGIMNLSQAIGVIMGANIGTTVTAQLVSF
                     NLEGIAPIALGIGILFYLFTSNQKTKHLSEILIGFGILFTGMEFMKDAVAPLAEYKAF
                     TDALLYFSKNPVLGILAGFAITGIVQSSSASMGMLIALASQGILPLSSALPILYGDNI
                     GTCVTSLLSSVGASRNARRAAVMHLSFNVIGTIIFMLVLNKPISAIVTHFDPTDTARQ
                     IANAHTLFNLTNVIILLPFSKYIVKLANRLIPIKETESEIVNNTKYLDERMFSTPSIA
                     LGNTVQEVVRMGHKATNSLEHSIAGFLNKSNEDINKAFESEKVVNKLQKDILNYLLKL
                     SKEPLRDDERFRTDLLFNTVNDIERVSDHAENIAELAMSVKEMNISFSDSAIREIYEI
                     YNKTITNFKDALIVLDVKDFELANKVLEVENEVNYLEKTFRNSHMIRLNNGSCTIDAG
                     VLYLDLLTNLERISDHSTNIVKQVLKLKQKI"
BASE COUNT          752 a          251 c          319 g          745 t
ORIGIN      
        1 attgacaaaa tttgttattc tatattatgg aaacaataaa ttttagttaa taaagtttat
       61 aattcagata aaaaaattat taatttctta agggaggaaa tatcgtgaat atagtaatta
      121 gccttatcgg tggacttgga ctttttcttt acggaatgag tttaatggga gaaggtttac
      181 aaaaatcagc aggagacaaa ttaaaaaaaa ttattgaact cttaacaagt aatgttgtta
      241 tgggtgtact ggtgggtaca gtcgttacag gtattataca aagttcaagt gctactactg
      301 ttatggttgt aggatttgta aatgcaggta taatgaatct aagtcaagct attggtgtaa
      361 taatgggggc aaatataggt actacagtta ctgctcaatt agtttcattt aatcttgaag
      421 gaattgcacc aattgcttta ggtataggta ttttattcta tttatttaca tcaaatcaaa
      481 aaacaaagca tttatctgag atacttatag gtttcggtat attatttaca ggaatggagt
      541 ttatgaaaga tgcagtagct ccacttgctg aatataaagc attcactgat gcacttttat
      601 attttagtaa aaatcctgta ttaggtatat tggcaggatt tgccataact ggtattgttc
      661 aaagttcaag tgcatctatg ggtatgttaa tagcactagc ttctcaagga atacttcctc
      721 tatcatctgc tttacctatt ctttatggag ataatatagg tacttgtgtt acttcacttt
      781 tatcaagtgt tggtgcaagt agaaatgctc gacgtgcagc tgtaatgcat ttaagtttta
      841 atgtaattgg tacaataata tttatgcttg ttttaaataa acctatatca gccatagtta
      901 ctcattttga cccaactgat acagctagac aaatagcaaa tgcacatact ttatttaatc
      961 ttacaaatgt tataatatta ttaccatttt ctaaatacat agttaaatta gcaaatagac
     1021 ttatacctat taaagaaact gaatcagaaa tagtaaataa tactaagtac ttagatgaaa
     1081 ggatgtttag cactccatct atagcacttg gtaatactgt acaagaagtt gtaagaatgg
     1141 gtcataaagc tacaaactct ttggaacatt caattgcagg atttttaaat aagagcaatg
     1201 aagatattaa taaagctttt gagtcagaaa aagttgtcaa taagttacag aaagatatat
     1261 tgaactattt gttaaaactt tctaaagaac ctcttagaga tgatgaacgc tttagaacag
     1321 atttgctttt taatactgta aatgacattg aaagagtctc tgaccatgca gaaaatatag
     1381 ctgaattggc catgagtgtt aaggaaatga acattagttt ttctgatagt gcgataagag
     1441 aaatatacga aatatacaat aagacaatta ctaattttaa ggatgcctta atagttttag
     1501 atgttaaaga ttttgagtta gcaaataaag ttttagaagt tgaaaatgaa gtaaattatt
     1561 tagaaaaaac ttttagaaat tcacatatga ttagactaaa taatggaagt tgtacaattg
     1621 atgctggtgt actttatctt gacttactta ctaatctaga aagaatttct gaccactcaa
     1681 caaatatagt aaaacaagta ctaaaactta aacaaaaaat atagtgatta tttttatata
     1741 taatcttaat caatgagaag taagaagcta tagataatgc ttatatcatt tatggcttct
     1801 tattttttat gtaatactca aatttttata gtattctctt attattaaac agtttctgcc
     1861 tagaatattc catatatata tgacttcttt cataaaactt gaactgccca taaataaagt
     1921 cacaagtata cataactatt ttttaatcaa ttataaccaa tatttgtttt atttatcttc
     1981 attttaattc ttcattcaat aatgtaataa gattattttt tatttggata ctattatatc
     2041 atctaaatat gttattatat atttgga
//
LOCUS       DBGJFF010000269         2061 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.274, whole genome shotgun sequence.
ACCESSION   DBGJFF010000269 DBGJFF010000000
VERSION     DBGJFF010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.274"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(113..991)
                     /locus_tag="ACOIAF_003853"
     CDS             complement(113..991)
                     /locus_tag="ACOIAF_003853"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HGZ2670827.1"
                     /translation="MTKEKFTGNPAGVITNADGLSESQMQKIARELNNSETAFIFNNL
                     AKNHQENYDVHIRFFTPTNEVPICGHATIAAHYVRAVENQFNNKTVFQKTGAGILPVD
                     IIKKDNDYEIIMTQGKIEVSPPLQQTYQKRLLNAFGILKEDIRTDCPIAISSTGHSKV
                     MIGIKELSTLNSLSPNLYELSKISQEIKCNGYYIFTLHNKETLQVHGRMFAPAIGISE
                     DPVTGNANGPLGAYLVHYKVIPTPNTEFIFEAVQGEAIKRKGKMLVRVIILDNKPVEV
                     KIIGKAVIAFSTNIEI"
     gene            1282..1638
                     /locus_tag="ACOIAF_003854"
     CDS             1282..1638
                     /locus_tag="ACOIAF_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HGZ2670828.1"
                     /translation="MDIDNNFEEFPNIEERQKDFNCSVGFTMTVIGSKWRAIILWHIL
                     KQEPIRYSQLKKEVVHISHKILSQELKYLERDGLIKRISYPTIPPKVEYLSTKRGKSL
                     ESILLELCNWGKQYMR"
     gene            complement(1753..1929)
                     /locus_tag="ACOIAF_003855"
     CDS             complement(1753..1929)
                     /locus_tag="ACOIAF_003855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670829.1"
                     /translation="MFMDKMDRCTHILTAYICSSRDYCNFIDTQLNDFILEYGENVVE
                     SCLHQVMVLVSRYN"
     regulatory      complement(1982..>2061)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
BASE COUNT          729 a          277 c          276 g          779 t
ORIGIN      
        1 tgataaatat atattaacaa ataataaaaa aattaatttg attgtatata aattagtttg
       61 ttattaaatt agtattaaaa agttcaaaaa tttacttaga aggaatctat aattagattt
      121 ctatgtttgt tgagaatgca attacagctt ttccaattat ttttacttca actggcttat
      181 tatctaaaat aattactctt actaacatct tgcctttgcg ttttattgct tctccttgaa
      241 ctgcttcaaa tataaactct gtgttgggtg taggtattac tttataatgt acaaggtacg
      301 cacctaaggg tccattagca tttcctgtaa cagggtcttc tgaaatacca attgcaggag
      361 caaacattct tccatgaact tgtagagttt ctttattatg taatgtaaaa atataatatc
      421 cattacattt tatttcttga cttattttcg ataattcata taaatttgga gataaactat
      481 ttaatgttga taattccttt attccaatca taacttttga atgtcctgta gatgatatgg
      541 caataggaca atctgtacga atatcttcct ttaaaatacc aaatgcatta agtaatcttt
      601 tttgataggt ttgttgtaat ggtggagata cttcaatttt tccttgtgtc ataattattt
      661 cataatcatt atcttttttt ataatatcaa ctggtaatat tccagcacca gttttttgaa
      721 atactgtttt gttattgaat tggttttcta ctgctctaac ataatgggca gcaatagtag
      781 catgaccaca tataggaacc tcattagttg gtgtaaaaaa tctgatatgt acatcataat
      841 tttcttgatg atttttagcc aagttattga aaataaaagc agtttctgaa ttatttaatt
      901 ctcttgctat tttttgcatt tgtgattcag ataagccatc agcatttgta attacaccag
      961 ctggattacc tgtaaatttt tcttttgtaa tgagtcaact tgataaagac gaaatttttt
     1021 attcatatta tataatcctt tctaaacatt agattagttt atctacatag aaaacttgag
     1081 ataaattatt atttattatt atctgtttaa ataaaattaa taaaataaca tatgtctata
     1141 ttaaagttta aacatataca tctaaaaaag tagtattatg taaggctcta tactacaatc
     1201 aagattatat tacgaattag tgcgctaaac aagaatgcac tttttagtta gatacttact
     1261 attttgaaag agaggattga gatggatata gataataatt ttgaagaatt tccaaatatt
     1321 gaagaaagac aaaaagattt taattgttca gttggtttta ccatgactgt gataggaagt
     1381 aaatggaggg caattatttt atggcatatt ttaaaacaag agccaatacg ttacagtcaa
     1441 ttaaaaaaag aagttgttca tataagtcat aagatactgt cacaagaatt aaagtatctt
     1501 gaaagagatg gacttattaa gaggatttct tatccgacta ttcctccaaa agttgaatat
     1561 ttgtctacta aacgtggtaa atcattggaa tctatattat tagaattatg taattggggt
     1621 aaacagtata tgagataaac tcaactgttt actttatgaa aaatatataa tgaagcttat
     1681 tttaattagt tctttaatga attataaaac gtagataaaa ctaaaaaggg ttttctagaa
     1741 aataaaattt ctttaattat atctgcttac caataccatc acttgatgca aacaagattc
     1801 taccacattc tctccatatt ctagtataaa atcatttaat tgtgtatcta taaaattgca
     1861 gtaatcgcgt gaactgcata tatatgcagt tagtatatgt gtacatctat ccattttatc
     1921 cataaacata tatcatatat ccccctaaaa aacattcata aaaacaaaaa gaaaatatct
     1981 ctttggcaac tggctgacat aactcatttc ataagtctta gtccctatag ctttgcgtca
     2041 ctaaatttct ctagttttgc c
//
LOCUS       DBGJFF010000270         2020 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.275, whole genome shotgun sequence.
ACCESSION   DBGJFF010000270 DBGJFF010000000
VERSION     DBGJFF010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.275"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            225..839
                     /locus_tag="ACOIAF_003856"
     CDS             225..839
                     /locus_tag="ACOIAF_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HGZ2670830.1"
                     /translation="MEEVIAKIITIENGFKEIENIAKKIVKNNDSKKCYYLSIYLYRS
                     EYYQVRELAVFILGFISVTISEALLFLKDNVSKDENWRVQEILAKSFDYYCSEVGYEK
                     ALPVIKEWLNSDSPNIKRAVTEGLRIWTSRDYFKSNPDVAISLLSSLKEDDSEYLRKS
                     VGNALRDISKKYPDLVKKELDSWDISNKKVEFVYKLASKCILAK"
     gene            857..1219
                     /locus_tag="ACOIAF_003857"
     CDS             857..1219
                     /locus_tag="ACOIAF_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HGZ2670831.1"
                     /translation="MKLRDEYTCPLELTHDMIKGKWKSIILWRLRIGPTSLSKLEKEI
                     NGINQKMLLEQLKELIEFGLVDKKTYEGYPLKVEYSLTPIRGKQILDALAIMQDIGIS
                     FMLDNGMEDILKEKGVLK"
     gene            1325..1822
                     /locus_tag="ACOIAF_003858"
     CDS             1325..1822
                     /locus_tag="ACOIAF_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HGZ2670832.1"
                     /translation="MKVTSTGIVNGVIEDKYGKRGTQFNEGGMPTYSLPLKFEDAPKN
                     TVSFAVFLEDKDSVPVCGFAWIHWLAVNITKDELNENESVTATDFVQGTTSWHGKLGG
                     LDRLEASVYGGMAPPDRPHVYEIHVFALDTILDLEKGFYMNEFFKAIEGHVLDTFTLK
                     GSYSN"
BASE COUNT          764 a          222 c          335 g          699 t
ORIGIN      
        1 tataactcaa ttaatttttt aaatacaact ttataatata aaacttataa taaaagtaaa
       61 tttatatgtt aaatgaggtt gattttttat tattttttat ttatgctagt actaaagact
      121 tgcaaacgtt ttttgacaag tctttatttt gtctaaatta aacaaacttt tttacaaata
      181 aatacataaa atttaaacta atctatttat tggaggagat aaaattggaa gaagtaattg
      241 caaaaattat tactatagaa aatggattca aagaaattga aaacattgct aaaaaaattg
      301 taaaaaataa tgactcaaaa aagtgttatt atctatcaat ctacttatat aggtcagaat
      361 attatcaagt aagagaattg gctgtgttta tacttggttt tatatcagta actatttctg
      421 aagctttgtt atttttaaaa gataatgtga gtaaagatga aaactggaga gtacaagaaa
      481 tactagctaa atcatttgat tattattgca gtgaagtagg atatgaaaag gctcttcctg
      541 ttataaaaga atggcttaat tctgatagtc caaacataaa aagggctgtg acagaaggtc
      601 ttcgaatttg gactagtaga gactatttta aatctaatcc tgacgttgct atatcactac
      661 tttctagttt aaaagaagat gatagcgaat acttgagaaa gtctgtaggt aatgctctta
      721 gagatataag taaaaaatat ccagatttag taaaaaaaga attagattca tgggatatat
      781 caaataaaaa agttgagttt gtatataagc ttgcttcaaa atgtatcttg gcaaagtaaa
      841 atggaggtga acttatttga aacttcgtga tgagtatact tgcccattag aactaacaca
      901 tgatatgata aaaggcaaat ggaaatctat tatattatgg agattacgca taggaccaac
      961 ttccttatca aaacttgaaa aggaaatcaa tggtattaat caaaagatgt tacttgaaca
     1021 acttaaagaa ttaattgaat ttggtcttgt agataaaaag acatatgaag gttacccact
     1081 taaagttgaa tactcactta cacctattcg agggaaacaa atattggatg ctttggctat
     1141 aatgcaagat ataggaatta gctttatgtt ggataatggt atggaagata ttcttaagga
     1201 aaaaggtgtt ttaaagtaaa tagataccca caaaaaagta agtaatttac cattattttc
     1261 aactggtata taattaaatt atagatatat tctattaatt tattataata ggaggtaaat
     1321 tattatgaaa gtaactagta caggtatagt aaatggtgtt atagaagata aatacggtaa
     1381 aagaggaact cagttcaatg aaggtggtat gcctacatat tcactacctc taaagtttga
     1441 agatgctcca aagaatacag tatcctttgc tgtattttta gaagataaag actctgttcc
     1501 tgtatgtggg tttgcatgga tacattggtt agctgtaaat ataacaaaag atgagttaaa
     1561 tgaaaatgaa agtgtaacag ctactgattt tgtacaaggg actactagtt ggcatggcaa
     1621 actaggaggt cttgacagat tagaggccag tgtatatggt ggaatggctc ctccagacag
     1681 acctcatgtc tatgaaatac atgtatttgc acttgatact atccttgacc ttgaaaaagg
     1741 attctatatg aatgaatttt tcaaagctat tgaagggcat gttttagata catttacact
     1801 aaaaggaagt tattctaatt aattaggttg actatagcta atcattaatt aaatgctaca
     1861 tttaattgat gattagctta attgttatta gaatattttc tgcactttat cttaatatat
     1921 attttagttg actggtattt taatttatat tagattatta tcaatttata taaattgatt
     1981 ttagcttaaa acttacttat ctatttcagt ttctattaga
//
LOCUS       DBGJFF010000271         1987 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.276, whole genome shotgun sequence.
ACCESSION   DBGJFF010000271 DBGJFF010000000
VERSION     DBGJFF010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.276"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            117..491
                     /locus_tag="ACOIAF_003859"
     CDS             117..491
                     /locus_tag="ACOIAF_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGZ2670833.1"
                     /translation="MNIVISNSSGKPIYEQITTQIKSMIISGELPEGSALPSMRLLAK
                     ELRISVITTKRAYSDLERDGFIETVTGKGSFVSSKNMDFVKEEQFRLIEEYLQKAVDT
                     AKSSDITYDELKDILFLLYKGE"
     gene            488..1351
                     /locus_tag="ACOIAF_003860"
     CDS             488..1351
                     /locus_tag="ACOIAF_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2670834.1"
                     /translation="MISMDAITIKNLNKTYKDFSLQDISFSVPKGSVMGFVGENGAGK
                     TTTLKAILNLISYDSGNIEIFGLDSKKNEKEIKEQIGVVFEGSNFHENLNTDHVSKIM
                     SKIYKNWNDTLFKDYLKKLRVPDNKLIKEFSKGNKMKLSIAVALSHKPKLLILDEATS
                     SLDPIVREEILDIFLDFIQDEEHSIILSSHITSDLDKIADYITFIHKGKIVFSENKDE
                     LIDTMGVLKCKPSDFDNLSREDYSYYRKSQFGYEVLLKDKHRFISRHPNCIVDNTSIE
                     EIMLFYVRGDK"
     gene            1351..>1987
                     /locus_tag="ACOIAF_003861"
     CDS             1351..>1987
                     /locus_tag="ACOIAF_003861"
                     /inference="COORDINATES: protein motif:HMM:NF024741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HGZ2670835.1"
                     /translation="MKGLILKDLLNLKGNIKFILLFIIMFGFMSSLGDGNVNNFIGVI
                     IVLCTTMIVSTFSYDDLNKWDSYVLTMPINRNDIVLSKYLTMLIFSFIGVLVSLIVSV
                     TIGYFKNTLILNETLLINALILSISVCFGSLILPLIYKFGTERARLLMILCFLVPTLA
                     LLVFKSILENISSPISIEIILNTLVYSLPFVAILLFVISYFISSKIYSKKEV"
BASE COUNT          758 a          207 c          303 g          719 t
ORIGIN      
        1 tacatttatt aaatgtataa tattggtaat tagttttttc taaaatcagt tgacatatta
       61 tatatatatt tatataatgt gtatatacta tatatacaat ttaaggaggt gtcttattga
      121 atatagttat tagtaattcg agtgggaagc caatatatga acagattact acacaaatta
      181 aaagtatgat tataagtgga gagttaccag aaggctcagc tcttccaagt atgagactat
      241 tggcaaaaga acttagaata agtgtaatta ctacaaaacg agcatatagc gatttagaac
      301 gagacggatt tattgagaca gttactggta aaggcagttt tgtatctagt aaaaacatgg
      361 attttgtcaa agaagaacaa tttcgtttaa ttgaagaata cctacaaaaa gcagttgata
      421 cagcaaaaag tagtgatatt acatatgacg aattaaaaga tattttattt ttattatata
      481 aaggagaatg ataagtatgg atgcaataac tataaaaaat ttaaataaaa cttataaaga
      541 ttttagccta caagatatat ctttttcagt accaaaaggg agtgtaatgg gatttgtagg
      601 tgaaaatggt gcaggaaaaa caactacatt aaaagcaatt ttaaatttaa tatcttatga
      661 tagtggaaat atagaaatct ttggtcttga tagtaaaaaa aatgaaaaag aaattaaaga
      721 gcaaattggg gttgtatttg agggaagcaa ctttcatgaa aatttaaaca ctgaccatgt
      781 ttcaaaaatc atgagtaaaa tctataaaaa ttggaatgac accttattta aagactactt
      841 aaaaaaattg agagtaccag acaataaatt aattaaggag ttttcaaagg ggaataagat
      901 gaaacttagt attgctgtag ccttatctca taaaccaaaa ttattaatac tagatgaggc
      961 aactagttca cttgacccta tcgttcgtga agaaatacta gatatatttt tagattttat
     1021 acaagatgaa gaacactcta ttatcttatc ttcacatatt acaagtgatt tagataagat
     1081 agcagactat attacattta tacataaagg aaaaattgta tttagtgaaa ataaggatga
     1141 attaattgat actatgggag ttttaaaatg taaacctagt gattttgata acctttcaag
     1201 agaggattat tcatattatc gtaaaagcca atttggatat gaagtacttc ttaaagataa
     1261 acatagattt atctcaagac atccaaactg tattgttgat aatacgtcta tagaagaaat
     1321 tatgttattt tatgtgagag gtgataaata atgaaaggtc taattttaaa agatttactt
     1381 aatttgaaag gaaatataaa gtttatactt ttattcatca ttatgtttgg ctttatgtct
     1441 tctttaggag atggaaatgt taataacttt ataggtgtta ttatagtttt atgtactaca
     1501 atgattgttt caacattttc atatgatgat ttgaataaat gggatagtta tgtacttaca
     1561 atgcctatta atcgcaatga tattgtatta agtaaatatt taactatgtt aatatttagc
     1621 tttataggtg ttcttgtttc acttattgtt agtgtaacta ttggttattt taaaaataca
     1681 cttatattaa atgaaacact acttattaat gcactaatct tatcaatttc agtatgtttt
     1741 ggtagcctta ttctaccact tatttataaa tttggtacag aaagagctcg tttattaatg
     1801 attttatgct ttttagttcc aacactagct ttattagttt ttaaaagtat tttagaaaat
     1861 attagttcac ctatttctat agaaattata cttaatacac tagtatattc tcttccattt
     1921 gttgctattt tactatttgt gatttcatat tttatatcat caaaaatcta tagtaaaaaa
     1981 gaagtat
//
LOCUS       DBGJFF010000272         1986 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.277, whole genome shotgun sequence.
ACCESSION   DBGJFF010000272 DBGJFF010000000
VERSION     DBGJFF010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.277"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            238..522
                     /locus_tag="ACOIAF_003862"
     CDS             238..522
                     /locus_tag="ACOIAF_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670836.1"
                     /translation="MLVYNKSFYPNDIFPRLDFSKIKKQLKLIDNDLSDFGSICIIEK
                     EHYTISVNSIGEINVYYDLEYENKVYRIVYEIEKLFKSQVGRFSISTYRN"
     gene            complement(1291..1506)
                     /locus_tag="ACOIAF_003863"
     CDS             complement(1291..1506)
                     /locus_tag="ACOIAF_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGZ2670837.1"
                     /translation="MAIVVNLDVMMAKRKISLKDLAEKIDITNANLSILKTGKAKAIR
                     FTTLNEICKALDCQPGDILEYVEDEND"
     gene            complement(1517..>1986)
                     /locus_tag="ACOIAF_003864"
     CDS             complement(1517..>1986)
                     /locus_tag="ACOIAF_003864"
                     /inference="COORDINATES: protein motif:HMM:NF022636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HGZ2670838.1"
                     /translation="MITKFFSNILSIFLYFLLSLLGVAGIIFLCMIPMFLYDFSSDKV
                     IALIARILYFSDYFVVILFLIFIIDSTKYSPFILENVKRFKAMGCCLLINTIIECING
                     YNSNTSLIMIFGDNSGGISPLMVISFIFALMCFVIAETFDKAIKIKEDNDLTI"
BASE COUNT          810 a          212 c          245 g          719 t
ORIGIN      
        1 tattaattaa taaaaagata gtagctattt ttatatagct attatttttt tattgttgtg
       61 gataaaaata tacttatatt ttactaatat acatttaaaa attacaattt aaaacattaa
      121 agaataaatt tgtattttgt tttatataaa gtaatttgaa aaatatggaa aacatgattg
      181 ctacattatt aattagggta taaaatggta taaatagaat gataaggggt ggatgaaatg
      241 cttgtatata ataaaagttt ttatcctaat gacatatttc caagattaga tttttcaaaa
      301 ataaaaaaac agttaaaact gatagataat gacctgtcag attttggaag catatgtata
      361 atagaaaaag aacattatac aataagtgta aacagtatag gtgaaataaa tgtgtattat
      421 gatttagagt acgaaaataa ggtgtataga atagtttatg agattgaaaa gttatttaaa
      481 tctcaagttg gaaggtttag tatatctaca tacagaaact gataattaaa aagtagaaat
      541 taaaaaactt aatactaaag gtatagataa atatatgagg taacagctag agagagttgt
      601 tacccttttt aaatactaag tatatattat tcttattaat tttttgtaaa ttcactaaaa
      661 gaccttttta tagaatatta ttttcttttt ttattaaata ggtaaatcag taaaaatgca
      721 attccagttc caattattat cccaatgata ttttctataa ttaatttttt taatataaaa
      781 gtaaataata aaaatgctat acataaacct aaaatatacc ctttaaaata gattgaaagt
      841 tttcttttca tataatcacc aacttgtatc agatttagtt tacttaatta gagtataaca
      901 gtgttgtaac ttcaaagagt atctaaaaga ttacaaatgt tagataaaat catcaataat
      961 gtaatttata acacgtagct ataaactatg caataatata aatggaggta taagtaaatg
     1021 aaaagttaca tgttttgcta acagtttaaa taaatattag gtaaaaaaga atgtataaat
     1081 gtaggattct gtggtaaaat tatcaattta aaagtatgat ttagtgtgat acagttctta
     1141 tgggtaatag ataacaaata gataaaggaa cagtttctta gaacatattt tgttatgact
     1201 ttaaacaaag ttaatattaa tgtcaataat atagtaaaag aataaaaacc atatagtata
     1261 ataaaaaaga gtgtctctaa acactctttt ctaatcattt tcatcttcta catattcaag
     1321 tatatcacct ggctgacagt ctaaagcttt gcaaatttca tttagagtgg taaatctaat
     1381 agcttttgct tttccagttt ttaaaattga taaatttgca tttgtaatat ctattttttc
     1441 tgccaaatct ttaagtgata ttttcctttt tgccatcatt acatctaggt taactactat
     1501 tgccataaca tcacctctat atcgttaaat cattatcttc ttttattttt atagctttat
     1561 caaatgtttc tgcaattaca aagcacatta gtgcgaatat aaaagaaata accataagtg
     1621 gtgagatacc gccactatta tctccaaata tcattataag actagtattt gagttataac
     1681 cattgataca ttcaattata gtgtttatca ataaacaaca tcccattgct ttaaaccttt
     1741 ttacattttc aagtatgaat ggggaatact tagtactatc aattataaat atcaagaata
     1801 agatgactac aaagtagtca gaaaaatata gtattcttgc aattaaagcg attactttgt
     1861 cagaactaaa atcatataaa aacataggta tcatacataa gaatattata ccagcaacac
     1921 ctaaaagtga taatagaaaa tatagaaata ttgataatat attactaaaa aattttgtaa
     1981 tcataa
//
LOCUS       DBGJFF010000273         1938 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.278, whole genome shotgun sequence.
ACCESSION   DBGJFF010000273 DBGJFF010000000
VERSION     DBGJFF010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.278"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            536..1864
                     /locus_tag="ACOIAF_003865"
     CDS             536..1864
                     /locus_tag="ACOIAF_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HGZ2670839.1"
                     /translation="MGSNELKKTLGVSAALSTVVGSVIGAGVFFKPQAVYTLTGGAPG
                     LGILAWLIAGIITITAGLTAAEVSVAIPKTGGMMVYIKEIYGEKLGFLTGWMQIVLFY
                     PGMMAALGVIFGEQASALIGSPSLLLPIAIGIIIIVAGLNMLGSKTGGVIQTVSTICK
                     LIPLILIMVVGFIKGGGNNPILTPMVGEGLSLGSVLGQVLIAILFAFDGWMNVGTLAG
                     EMKNPGKDLPKAIIGGLSVVMAVYFIINLAYLWVLPANELANYASPASAVAEVIFGSM
                     GGKIISVGILISVFGALNGFLLTGSRVAYTLATDKTLPKYGIFSKLNSAQVPANAIAL
                     VSVIASIYALSGQFNLLTDLAVFATWIFYVLTFIGVMKLRKTHPNIPREYKVPLYPIV
                     PIIAIASGIFVVVNQLCFAGMKTTMISIGGLVITAIGLPVYAYMTRGVKR"
BASE COUNT          692 a          218 c          337 g          691 t
ORIGIN      
        1 atttagattt taacatctaa aaggagtttt caaacctaat atttcatttt taaataaaaa
       61 aataaaatat tagttttgat ttctggttta aattgggtta taataaattg tcaaattatt
      121 tattaatata aaatagacta taaaacaagc aaaaatcata ctattatata aaagacctta
      181 aagatagaaa actacagaaa gaagccaaaa tgtttttgaa aaaaatgaaa ttttattgca
      241 aaaaaatcaa aatgaattta acttaatagt tataatctat ttttagtgtt acaaatatac
      301 aatgtatatt atacaatata aaaaatacat ttacttagaa gtattattat atttggattt
      361 ttggttttta taattttttt atatttaaac ataactaaaa ataaaagatt acgaaagcta
      421 agtagagttt attttatttt aataaataat aaagctattt ttctatatat tggcataata
      481 tatgcttatt taactatgaa attaaaatta aataggatta atgaggagga aaaaaatggg
      541 aagtaatgaa ctgaaaaaga ctcttggcgt atcagcagca ttatcaactg ttgttggttc
      601 cgttatagga gcgggcgtat tttttaaacc acaagcagtc tatacattaa ctggaggagc
      661 tccaggtctt ggtatattgg catggttaat agctggaatc ataactataa cagctggtct
      721 tacagctgca gaggtatcag ttgcgatacc aaaaactggt ggaatgatgg tatatataaa
      781 agaaatttat ggagaaaaat taggtttttt aactggatgg atgcaaattg tattatttta
      841 tccagggatg atggcagcat taggtgttat atttggagaa caagcatcag cactaattgg
      901 tagtccatca cttttacttc caatagcaat tggaattata ataatagttg cagggcttaa
      961 tatgttaggt tcaaaaactg gaggagtcat acaaactgtt tctacaattt gtaagttaat
     1021 accacttatt ttaataatgg ttgttggatt tattaaaggt ggtggaaata atccaatact
     1081 tacacctatg gttggtgaag gattaagcct gggtagtgta ctaggtcaag ttttaattgc
     1141 tatattattt gcttttgatg gatggatgaa tgttggtact ttagctggag aaatgaagaa
     1201 tcctggaaaa gaccttccaa aagctataat aggtggattg tcagttgtta tggctgttta
     1261 ttttataata aatttagcgt atctttgggt attaccagct aatgaattag caaattatgc
     1321 ttctcctgca tcagcagttg ctgaagttat atttggttca atgggtggaa aaataatatc
     1381 tgtaggtata ttaatttcag tgtttggagc attaaatgga tttttactaa ctggttcaag
     1441 ggtggcatat actttagcta cagataagac tttaccaaag tatggtatat tttctaaatt
     1501 aaattctgct caagttccag caaatgcaat agcattagta tctgttattg catctatata
     1561 tgctttgtca ggtcaattta atttattaac tgacttagct gtatttgcta cttggatatt
     1621 ctatgtatta acttttatag gggttatgaa acttagaaag actcatccaa atataccaag
     1681 agagtataaa gttccattat atcctattgt tcctataata gctatagcaa gtggtatatt
     1741 tgttgtagtt aatcaattgt gttttgcagg tatgaaaact actatgatat ctataggagg
     1801 tcttgtaata acagctatag gcttaccagt gtatgcttat atgactagag gagtaaaaag
     1861 ataaaccaag tagtttacaa atgcttcata attaaaataa aagagactgt ctcaaataga
     1921 gataagtctc ttttttat
//
LOCUS       DBGJFF010000274         1934 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.279, whole genome shotgun sequence.
ACCESSION   DBGJFF010000274 DBGJFF010000000
VERSION     DBGJFF010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.279"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            119..235
                     /gene="rrf"
                     /locus_tag="ACOIAF_003866"
     rRNA            119..235
                     /gene="rrf"
                     /locus_tag="ACOIAF_003866"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            242..316
                     /locus_tag="ACOIAF_003867"
     tRNA            242..316
                     /locus_tag="ACOIAF_003867"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:274..276,aa:Asn,seq:gtt)
     gene            321..395
                     /locus_tag="ACOIAF_003868"
     tRNA            321..395
                     /locus_tag="ACOIAF_003868"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:354..356,aa:Glu,seq:ttc)
     gene            401..476
                     /locus_tag="ACOIAF_003869"
     tRNA            401..476
                     /locus_tag="ACOIAF_003869"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:434..436,aa:Val,seq:tac)
     gene            482..558
                     /locus_tag="ACOIAF_003870"
     tRNA            482..558
                     /locus_tag="ACOIAF_003870"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:516..518,aa:Asp,seq:gtc)
     gene            569..643
                     /locus_tag="ACOIAF_003871"
     tRNA            569..643
                     /locus_tag="ACOIAF_003871"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:601..603,aa:Thr,seq:tgt)
     gene            658..742
                     /locus_tag="ACOIAF_003872"
     tRNA            658..742
                     /locus_tag="ACOIAF_003872"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:692..694,aa:Tyr,seq:gta)
     gene            752..825
                     /locus_tag="ACOIAF_003873"
     tRNA            752..825
                     /locus_tag="ACOIAF_003873"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:784..786,aa:Gly,seq:tcc)
     gene            836..912
                     /locus_tag="ACOIAF_003874"
     tRNA            836..912
                     /locus_tag="ACOIAF_003874"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:870..872,aa:Arg,seq:tct)
     gene            922..997
                     /locus_tag="ACOIAF_003875"
     tRNA            922..997
                     /locus_tag="ACOIAF_003875"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:955..957,aa:Gln,seq:ttg)
     gene            1009..1084
                     /locus_tag="ACOIAF_003876"
     tRNA            1009..1084
                     /locus_tag="ACOIAF_003876"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1042..1044,aa:Lys,seq:ttt)
     gene            1087..1175
                     /locus_tag="ACOIAF_003877"
     tRNA            1087..1175
                     /locus_tag="ACOIAF_003877"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1121..1123,aa:Ser,seq:tga)
     gene            1193..1283
                     /locus_tag="ACOIAF_003878"
     tRNA            1193..1283
                     /locus_tag="ACOIAF_003878"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1227..1229,aa:Ser,seq:gct)
     gene            1292..1368
                     /locus_tag="ACOIAF_003879"
     tRNA            1292..1368
                     /locus_tag="ACOIAF_003879"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1326..1328,aa:Pro,seq:tgg)
     gene            1454..1529
                     /locus_tag="ACOIAF_003880"
     tRNA            1454..1529
                     /locus_tag="ACOIAF_003880"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1487..1489,aa:Trp,seq:cca)
     gene            1590..1666
                     /locus_tag="ACOIAF_003881"
     tRNA            1590..1666
                     /locus_tag="ACOIAF_003881"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1624..1626,aa:Pro,seq:tgg)
     gene            1673..1749
                     /locus_tag="ACOIAF_003882"
     tRNA            1673..1749
                     /locus_tag="ACOIAF_003882"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1707..1709,aa:Ile,seq:gat)
     gene            1753..1828
                     /locus_tag="ACOIAF_003883"
     tRNA            1753..1828
                     /locus_tag="ACOIAF_003883"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1786..1788,aa:Phe,seq:gaa)
     gene            1836..1912
                     /locus_tag="ACOIAF_003884"
     tRNA            1836..1912
                     /locus_tag="ACOIAF_003884"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1870..1872,aa:Met,seq:cat)
BASE COUNT          489 a          443 c          526 g          476 t
ORIGIN      
        1 attcattaag atatatgtgc agtttttaga gtgttaactc taaaaaaaaa taaaaaaagt
       61 attgacaaaa atagtaaaaa atgttaatat tatacttgtc ctagcaacaa gaaaaaaatg
      121 tggttataat agcggagaag atacacctgt ttccattccg aacacagagg ttaagttctc
      181 tagcgctgat ggtacttggt gggaaactgc ctgggagagt aggacatagc cacgtgaatg
      241 ttccaaggta gctcaacggt ggagcaaccg gctgttaacc ggtaggctgt gggttcgagc
      301 cccacccttg gagccattat ggctcgttgg tcaagaggtt aagacaccgc cctttcacgg
      361 cggtaacagg ggttcaattc ccctacgagt caccatttgt gggatcatag ctcagctggg
      421 agagcacctg ccttacaagc agggggtcac aggttcgagc cctgttggtc ccaccatttg
      481 cggcctggta gttcagttgg ttagaatgcc agcctgtcac gctggaggtc gagggttcga
      541 gccccttcca ggtcgccaaa ataaaaatgc tggtgtggct caacggtaga gcagctgact
      601 tgtaatcagc aggttgtagg ttcgattcct atcaccagct ccatgtaaaa ctaatacgga
      661 ggatttcccg agcggccaaa gggggcagac tgtaaatctg ttgtcatcga cttcggtggt
      721 tcgaatccac catcctccac caattaaata tgcgggtgta gctcaatggt agagttctgg
      781 ccttccaagc cagctgtgag ggttcgatcc ccttcacccg ctccaaagaa agcgtgggtg
      841 catagctcag ctggatagag caacgccctt ctaaggcgtg tgtccggggt tcgaatccct
      901 gtgcgctcac cacatatata ttggggatta gccaagtcgg taaggcacat gactttgact
      961 catgtatgcg taggttcgag tcctgcatcc ccagccaaat aaaaatatgg tctattagct
     1021 cagtcggtag agcacctgac ttttaatcag ggtgtcccgc gttcgagtcg cggatagatc
     1081 accaatggag aggtgtccga gtggtttaag gagctggtct tgaaaaccag tgacttcgaa
     1141 aggggccgtg ggttcgaatc ccaccctctc cgccaaaaaa atgactgaat ttggagaagt
     1201 actcaagtgg ctcaagagga tcccctgcta agggattagg ctgggtaacc ggtgcgaggg
     1261 ttcgaatccc tccttctccg ccattcaaaa acgaggtgta gcgcagtttg gtagcgcaca
     1321 tggtttggga ccatggggcc gggggttcga gtcccttcac ctcgaccaac ttaagctttt
     1381 agttcagttg gttagagtgc ccaactcatg gctagtaatt ctagggtttg agtccatgaa
     1441 ggttattctt cataggggtg tagctcagtt ggtagagcgt cggtctccaa aaccgtgcgc
     1501 cgggggttcg agtccctcca cccctgccaa ataaaaagga cttgaaaaaa gttggatttt
     1561 agagtagtat aagatagtaa ataagatttc gaggtgtagc gcagtttggt agcgcacatg
     1621 gtttgggacc atggggccgg gggttcgagt cccttcacct cgaccaagaa aagggcctat
     1681 agctcaggtg gttagagcgc acgcctgata agcgtgaggt cgctggttcg agtccagcta
     1741 ggcccaccat ttgcccagat agctcagtcg gtagagcagg ggactgaaaa tccccgtgtc
     1801 ggtggttcga ttccgcctct gggcaccagg aatacggcgg tatagcttag ttggctagag
     1861 cgttcggttc atacccgaaa ggtcataggt tcgactccta ttaccgctac catctaacta
     1921 tatcaataaa gaac
//
LOCUS       DBGJFF010000275         1894 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.280, whole genome shotgun sequence.
ACCESSION   DBGJFF010000275 DBGJFF010000000
VERSION     DBGJFF010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.280"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            548..1819
                     /gene="serS"
                     /locus_tag="ACOIAF_003885"
     CDS             548..1819
                     /gene="serS"
                     /locus_tag="ACOIAF_003885"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006441208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HGZ2670840.1"
                     /translation="MLDIKRIRENLDGIKKAMERRGEKDFNLDEVVKLDDERRRILQE
                     VEVMKNELNTASKNIPNLIKEGKDVENEKIKLKQLSDKIKVIDQNLKEVEDKMEYLLM
                     RIPNVPHPEVPQGETDEDNVEVRTWGKTTTFDFESKAHWEIGTELGILDFETASKITG
                     SRFTLYKGLGARLERALLNFYLDTNTKVNGYTEVIPPFMANRNSFLGTGQLPKFEEDM
                     FKIEGLDYFMIPTSEVPLTNIHANEILKFEQLPINYTAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVEMVKIVAPEESYNELEKLTNNAETMLQLLNLPYRVVKICTGDLGFTASFK
                     YDVEVWMPSYNRYVEISSCSNCEDFQARRAGIRFKRDKDSKAEYVHTLNGSGLAIGRS
                     VAAILENYQQEDGSVIVPEVLRPYMGVSVIK"
BASE COUNT          798 a          195 c          316 g          585 t
ORIGIN      
        1 agactttgga actaaatcta taatagcaaa tttaaattta ttaaatagat taccttataa
       61 catttcatat aaaatatatt gtgaatctga gaaaattact atttttcttt gagtattttt
      121 tgtaagcagt tctaaaagat tcaatttcat acctattact tgaatcattc attaaaataa
      181 ttctaattta aaaaataatt ctatccaaat aatagatata taggactatg ttataatata
      241 aaaaaattta tataaaagtt aataactata caacattgat aaagaaaaag tcaaaataga
      301 taattgatta gcaaaataga tataattata atgaagttaa atctagcaat attctcctaa
      361 aattaaatta tatttaatta attaaatata atttgacaat agacaaaaaa gtgagtaaaa
      421 tataaataaa ataaaaataa caggttcgga taaagaaaaa ggtttcttaa cctcaagcat
      481 aaagtatatt atctaagtta tttgagatta agagataaat ccaatcttag gtactgagga
      541 ggaaaaaatg cttgatataa aaagaataag agaaaattta gatggtataa aaaaagcaat
      601 ggaaagaagg ggcgaaaaag actttaatct tgatgaagta gttaaattag atgatgaaag
      661 aagaaggatt cttcaagaag ttgaagttat gaaaaatgaa ttaaatacag catctaaaaa
      721 tataccaaat cttataaaag aaggtaaaga tgtagaaaat gaaaagatta aactaaaaca
      781 gttatctgat aaaattaagg taatagacca aaatcttaaa gaagtagaag acaagatgga
      841 atacctatta atgagaatac caaatgtacc acatccagaa gtaccacaag gtgaaactga
      901 tgaagataat gttgaggtaa gaacttgggg aaagactact acctttgatt ttgaatctaa
      961 ggctcattgg gaaattggaa cagaattagg tatacttgat tttgaaacag ctagtaaaat
     1021 aactggttca aggtttactc tttacaaagg attaggtgct agattagaga gagcattatt
     1081 aaatttttac ttggacacaa atacaaaagt aaatggatat acagaagtta ttccaccatt
     1141 tatggcaaat agaaatagtt ttttaggtac aggacagtta cctaagtttg aagaagatat
     1201 gtttaaaata gaaggtctgg attattttat gattccaaca tcagaagttc ctttgactaa
     1261 tatacatgct aatgaaatac ttaaatttga acagttacca ataaattata cagcttatac
     1321 tccatgtttt aggtcagaag ctggttctgc tggaagagat acaagaggtt tggtaagaca
     1381 gcatcagttt aataaagtag aaatggttaa aatagttgca ccagaagaat cttacaatga
     1441 gttagaaaag cttactaaca atgcggaaac tatgctacaa ttattgaatc tgccttatag
     1501 agtagtaaag atatgtacag gtgatttagg atttacggct tcttttaaat atgatgtaga
     1561 agtttggatg ccaagttaca ataggtatgt tgagatttca tcttgttcaa actgcgaaga
     1621 tttccaagct agaagagcag gaataagatt taaaagagat aaagattcta aagctgaata
     1681 tgtacacaca ttaaatggct ctggtctagc tattggaaga agtgttgcag ctatattaga
     1741 aaattatcaa caagaagatg gttctgtgat tgttcctgaa gttttaagac catatatggg
     1801 agtttctgtt attaaataag agttaaatta tgtattttta agcaatacaa taatatagag
     1861 gtaataaaaa ataattaata taaaagtgat actt
//
LOCUS       DBGJFF010000276         1853 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.281, whole genome shotgun sequence.
ACCESSION   DBGJFF010000276 DBGJFF010000000
VERSION     DBGJFF010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1853
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.281"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            69..185
                     /gene="rrf"
                     /locus_tag="ACOIAF_003886"
     rRNA            69..185
                     /gene="rrf"
                     /locus_tag="ACOIAF_003886"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            193..267
                     /locus_tag="ACOIAF_003887"
     tRNA            193..267
                     /locus_tag="ACOIAF_003887"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:225..227,aa:Asn,seq:gtt)
     gene            273..358
                     /locus_tag="ACOIAF_003888"
     tRNA            273..358
                     /locus_tag="ACOIAF_003888"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:307..309,aa:Leu,seq:taa)
     gene            374..449
                     /locus_tag="ACOIAF_003889"
     tRNA            374..449
                     /locus_tag="ACOIAF_003889"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:407..409,aa:Met,seq:cat)
     gene            457..531
                     /locus_tag="ACOIAF_003890"
     tRNA            457..531
                     /locus_tag="ACOIAF_003890"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:490..492,aa:Glu,seq:ttc)
     gene            546..619
                     /locus_tag="ACOIAF_003891"
     tRNA            546..619
                     /locus_tag="ACOIAF_003891"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:578..580,aa:Gly,seq:tcc)
     gene            625..700
                     /locus_tag="ACOIAF_003892"
     tRNA            625..700
                     /locus_tag="ACOIAF_003892"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:658..660,aa:Val,seq:tac)
     gene            706..782
                     /locus_tag="ACOIAF_003893"
     tRNA            706..782
                     /locus_tag="ACOIAF_003893"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:740..742,aa:Asp,seq:gtc)
     gene            793..867
                     /locus_tag="ACOIAF_003894"
     tRNA            793..867
                     /locus_tag="ACOIAF_003894"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:825..827,aa:Gly,seq:gcc)
     gene            877..950
                     /locus_tag="ACOIAF_003895"
     tRNA            877..950
                     /locus_tag="ACOIAF_003895"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:911..913,aa:Arg,seq:tct)
BASE COUNT          610 a          298 c          367 g          578 t
ORIGIN      
        1 agaaactagt attactaggt tctataaact tattaaaaac ttaatagaaa tattaagcat
       61 aaagattatg tggttacaat agcagagagg atacacctgt tcccattccg aacacagaag
      121 ttaagctctc tagcgctgat ggtacttggt gggaaactgc ctgggagagt aggacgtagc
      181 cacgttaaat gttccaaggt agctcaacgg tggagcaacc ggctgttaac cggtaggctg
      241 tgggttcgag ccccaccctt ggagccaaaa aagccgaagt ggcggaactg gcagacgcac
      301 aggacttaaa atcctgcggg acttacctct cgtaccggtt cgattccggt cttcggcact
      361 tataaaacaa tatcgcgggg tggagcagtt ggcagctcgt cgggctcata acccgaaggt
      421 cgtaggttca agtcctgcct ccgcaaccat taaaacggct cgttggtcaa gaggttaaga
      481 caccgccctt tcacggcggt aacaggggtt caattcccct acgagtcacc aattcaatta
      541 aatatgcggg tgtagctcaa tggtagagtt ctggccttcc aagccagctg tgagggttcg
      601 atccccttca cccgctccaa aaatgggacc atagctcagc tgggagagca cctgccttac
      661 aagcaggggg tcacaggttc gagccctgtt ggtcccacca ttcgcggcct ggtagttcag
      721 ttggttagaa tgccagcctg tcacgctgga ggtcgagggt tcgagcccct tccaggtcgc
      781 caaattaaat aagcgggaat agttcagtgg tagagcgcaa ccttgccaag gttgaagtcg
      841 cgagttcgaa tctcgtttcc cgctccataa taacttgggt gcatagctca gctggataga
      901 gcaacgccct tctaaggcgt gtgtccgggg ttcgaatccc tgtgcgctca ttaagaaaag
      961 tttactataa tttatagtaa acttttatat tgtttaaaaa gacacaggaa acatgtatat
     1021 tacctgtgtc tttttattta taaactttaa actatgtaaa aattacagta tattctaata
     1081 gagttacagt atattcatta atattgtaaa catacggagg gtaatgtata atagtctaca
     1141 agatgtttat gaaggcatca gttaaatata ataaatataa ctaattagta agagttatat
     1201 acattatatt gtatacttta tggtatttat atattgaaat aacttatgaa attatttata
     1261 aagagattca tgagcatatt tttgtctaaa caataagtat aagaaattat aaaaaattag
     1321 agactatagt gaatttacaa tttaatgtaa tatttataag ttatactaaa taaattatta
     1381 tactgtgaaa tggatatata actttaggta ttgtgtatta ttttcactgt aggagattat
     1441 atctagttat acataataag ttttaatgat agtttatagt aagatattaa aataaattta
     1501 tctaatctag atatgaaata agttattact ttaatataaa aatgaatcta atggaataat
     1561 attgatgata agtaatattt agattttgta tttgtttata gttaataagt ggattataga
     1621 atttataata attaatatat attaaacaaa gatatattaa gatattttag aacacaaaac
     1681 tgcattaagg cgcgagtact attttgtatt cgcgccttga tggtttgagt tatttgagta
     1741 aaaactaact ttaataagaa atctgtattt attaagttaa tatttttaat gtgtttagag
     1801 attatttaac aatctccaaa tataaaacta gaattattta cataaaagtt aaa
//
LOCUS       DBGJFF010000277         1852 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.282, whole genome shotgun sequence.
ACCESSION   DBGJFF010000277 DBGJFF010000000
VERSION     DBGJFF010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.282"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            330..1202
                     /locus_tag="ACOIAF_003896"
     CDS             330..1202
                     /locus_tag="ACOIAF_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HGZ2670841.1"
                     /translation="MEETNEMKDSKHLISNIQSQYTRLSKGQKLIAQYILNNYDKVAF
                     MTACKLGETVGVSESTVVRFANALGYSGYPKLQAALQELIKNKLTTVQRVEMAHDYSD
                     DFAILNKVLKSDIDNIRSTLEEIDERAFKEASNKLLKARKIYILGMRSSFVVAQYLGF
                     YLDIILDNVHIIRMDMGDAFEQIVRINEEDVIVAISFPRYSKKSYQIVNYAKEKGAHV
                     ISLTDSLFAPVASLADNTLLVKSNMASFVDSLVPALSISNALAISVGMKEKEDIKQHF
                     DDLEQIWKRYSVYE"
BASE COUNT          772 a          184 c          268 g          628 t
ORIGIN      
        1 atactttaaa taataagtat acaacagctt attctcaaaa caaaatatac atctaaatat
       61 gcaaaataag atagataact tttaaatgag taaaaattga aaaatttaaa agaaatattg
      121 gataaatatt aaaaaatgca aggaaagatg gtataataaa tttaatcgaa aatactatgt
      181 gaagtataag gtacagaaaa gttagaattt tatgagttta agaagctatg ataaaaacta
      241 agatgaaatt gagataaaaa ataaaatttt tagagaaaaa gacagtgtta ataaaacaaa
      301 atagtttata taactaaaag gagaaaaaaa tggaagaaac aaatgaaatg aaggacagta
      361 aacatttgat atcaaatata caatctcaat atacgagatt gagtaaaggt caaaaactta
      421 tagcacaata cattttaaat aattatgata aagttgcatt tatgactgca tgtaaattgg
      481 gtgaaactgt aggagtaagt gagtctacag ttgtaagatt tgcaaatgca ttaggttata
      541 gtggatatcc taaattacaa gctgcacttc aagaactcat aaaaaataaa ctaactacag
      601 tacaaagagt tgaaatggca catgattatt ccgatgattt tgcaatacta aacaaagttt
      661 taaaaagtga tatagataat ataaggtcta cactagaaga aattgacgag agagcgttta
      721 aagaagcttc taataaactt ttaaaagcga gaaaaatata tatacttgga atgaggagtt
      781 cttttgtagt agctcaatat ttaggttttt acttagatat aatacttgac aatgttcata
      841 taattagaat ggatatggga gatgcttttg agcagattgt aagaataaat gaagaagatg
      901 taatagttgc tattagtttc cctcgttatt ctaagaaatc ttatcaaatt gtcaattatg
      961 ctaaggaaaa aggagcccat gtaatatcat taacagatag cttatttgcg ccagtagcat
     1021 cacttgcaga taacactctt ctagttaaga gtaatatggc ttcatttgta gattctctag
     1081 ttccagctct tagtatttca aatgcccttg ctatatctgt tgggatgaag gaaaaagaag
     1141 atattaaaca gcattttgat gatttagagc aaatttggaa aagatattct gtgtatgagt
     1201 aaagtttgta tattaataag gctatggaat tgagaatcat agccttattg gtaaattctt
     1261 gattatcact taaaaaataa attttcgttt aaataataca aataaatatt tcgagactca
     1321 aaacataaag atgtattctg aatacatctt tactacgtca actgatttat taaagaaagc
     1381 atttcccttc atggagagtg ctttttttat ttctctaaac aatataattt ccttaattaa
     1441 caattataaa attaatttaa tttgtattta agtatataat atcttattaa atttctatta
     1501 aaatatgtat ttctatattt ttattcacca taatatgtgt cttttagtat tttattttaa
     1561 ctttgataaa gaacttgaaa aatttaaaaa tcataggatt attagttaag tatgaaatct
     1621 tgatttttta agtagctatt tttggggaaa gggaaacttt tttagaaata taatacttta
     1681 taaaaataaa ataaagtttt tataaatatt aagacataga atgtaaagct tttgtaataa
     1741 tgtatttacc aaaataatac aatatatctt atattataac tatatgaatt atataaatca
     1801 atacttaaac atagcaatag aggcatttta aaaaaacata aatttataat at
//
LOCUS       DBGJFF010000278         1784 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.283, whole genome shotgun sequence.
ACCESSION   DBGJFF010000278 DBGJFF010000000
VERSION     DBGJFF010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.283"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            <1..597
                     /locus_tag="ACOIAF_003897"
     CDS             <1..597
                     /locus_tag="ACOIAF_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2670842.1"
                     /translation="GLIKESSGQVLLNKHNLSNLAEHRRTQIVSRVFQDPSLGTCPSM
                     TVRENLSLALNKGKLLNLRKCLRYKRDFLENLLEGVSLDLKKYLDVQVKFLSGGQRQS
                     LSLIMSCLTNPSVLLLDEHTAALDPKTSNEVIELTNKIVREKNITTLMVTHNLKHALH
                     YGDRLIMLHKGEVVLDVKGKEKEQLTVEEILEKFEYAV"
     gene            728..1750
                     /locus_tag="ACOIAF_003898"
     CDS             728..1750
                     /locus_tag="ACOIAF_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD0873 family tyrosine ABC transporter
                     substrate-binding lipoprotein"
                     /protein_id="HGZ2670843.1"
                     /translation="MINKKRLASLILAGALSISMLTGCSQGGDSGNSKQESNSKGKEV
                     KKIGITQLVEHPALDATRTGFVKALEKNGFKDGENIDIDFQNAQNDMPTTQSIASKFA
                     SDKKDLIFAISTPSAQAAFNATKDIPILITAVSDPVAAGLVKSLEKPGTNVSGTSDFV
                     SVDKGLELLKIFAPKAKTIGVMYNTSEVNSKVQVDALKEYASKNGFKVVEKGITTSNE
                     VNQGISSLVGKIDVLYVPTDNLVASSMPIVSKIATENKIPVIAAESGPVEKGALACQG
                     INYEKLGYKTGEMAVKILNGESVSDMPVATSDDTDIIVNEDILKALGMEKPSNENISY
                     VKTKQE"
BASE COUNT          682 a          241 c          304 g          557 t
ORIGIN      
        1 gggcttataa aagaatcttc tggtcaagta ttattaaata aacataactt gtctaattta
       61 gctgaacaca gaagaactca aatagtaagt agagtttttc aagacccaag tcttggaact
      121 tgcccttcta tgactgttag agaaaacttg tctttagctc ttaacaaagg aaaactatta
      181 aatctaagaa aatgtcttcg ttacaaaaga gatttcttag aaaatttatt ggaaggtgta
      241 tctcttgatt taaaaaaata ccttgatgtt caagtaaaat ttttatcagg aggtcaaaga
      301 caatcattat cactaattat gtcgtgctta acaaatccat cagttttatt acttgatgaa
      361 catacagcag cacttgaccc aaaaacatca aatgaagtaa ttgaattgac taataagatt
      421 gtaagggaaa aaaatataac tacacttatg gttactcaca acttaaaaca cgctcttcat
      481 tatggtgata gattgataat gcttcataag ggtgaagttg tattagatgt aaaaggtaaa
      541 gaaaaagaac aattaacagt tgaagaaatc ttagaaaaat ttgaatatgc agtttaaaat
      601 aatatatcta ttgtattaaa ctcggcttta aaataaagtt aaaattacaa taattatggt
      661 tactttgtaa taattcatat gaatatatga acatatataa caataaattt ttgaggggga
      721 tataaaaatg attaataaaa aaagattggc aagtttgatt ttagctggtg ctcttagtat
      781 atctatgctt acagggtgtt cacaaggagg agactctggt aattcaaaac aagaaagtaa
      841 ttctaaaggt aaagaagtta aaaaaattgg tataactcaa ctggttgaac atccagcatt
      901 agatgccact aggacaggtt ttgtaaaagc attagaaaag aacggattta aagatggaga
      961 aaatattgat atagacttcc aaaatgctca aaatgacatg cctactacac aaagtattgc
     1021 gagtaaattt gcatctgata agaaggactt aatatttgct atatctactc catcagctca
     1081 ggcagcattt aatgctacta aagatatacc tatacttatt actgctgtga gtgaccctgt
     1141 tgctgctgga ttagttaaat ctcttgaaaa gccaggcaca aatgtatctg gaacatcaga
     1201 ttttgtttct gtagacaaag gtttagaact tcttaaaata tttgctccta aggccaaaac
     1261 tataggagtt atgtacaata ctagtgaagt aaattccaaa gttcaagttg atgctttaaa
     1321 agaatatgca tctaaaaatg gatttaaagt tgttgaaaaa ggtataacta cttcaaatga
     1381 agttaaccaa ggtatttcta gtttagttgg caaaatagac gtattatatg ttcctacaga
     1441 taacttagtg gcttcttcta tgcctatagt ctctaaaata gctactgaga ataaaatacc
     1501 tgttatagca gctgaatctg ggccagttga aaaaggtgct cttgcttgtc aaggtatcaa
     1561 ctacgaaaaa ctaggttaca aaacaggtga aatggcagtt aaaattttaa atggcgaatc
     1621 agtatctgat atgcctgtgg ctacatcaga tgatacagat atcattgtta atgaagatat
     1681 attaaaagct ttaggtatgg aaaaaccttc taatgaaaat atttcttatg taaagactaa
     1741 acaagaatag aaagatattt tctatatacg attaatatgc ctgt
//
LOCUS       DBGJFF010000279         1747 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.284, whole genome shotgun sequence.
ACCESSION   DBGJFF010000279 DBGJFF010000000
VERSION     DBGJFF010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.284"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            411..1616
                     /locus_tag="ACOIAF_003899"
     CDS             411..1616
                     /locus_tag="ACOIAF_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HGZ2670844.1"
                     /translation="MSKKVLIMSASTGGGHNRAALAIKEELTSKTLDGEPIECEIIDS
                     LKLVNNTMDKIISRGYEKSAIYTPKAYGSVYRLSETNLLSKNEFKDNLLITFMAKKFK
                     KLIRSEKPDLIIGTHPFPMIALSTLKKNFNLHNNESNAYTEHFYKHYTNTINVPPLIS
                     VLTDYTTHSTWIQNEIDYYIVGHEYVKELLVFDGVEPSKIRTFGIPVEKSFLSHRDKD
                     IVLSELNLSPDKLTVLLMGGSFGAGNIKETLDELLDTDRDFQILVITGKNESLKEKIE
                     KKLMSRYHDKNVCVLGYTDKMNDILASIDVLVSKPGGLTTTEALLKDVPMIVPYYIPG
                     QEEENLDFLSNCGAALRTTKKYNLSILLKVLIDDPSRLEMLKRNIKSIRKSNSAQNIA
                     NLILNIISG"
BASE COUNT          647 a          218 c          226 g          656 t
ORIGIN      
        1 gttcgtgttt atttcttttt ccttttttca aggacaagta atactatatc atctttatct
       61 attctagtca atataatttt ttactttttt taattttttt atttatcatg tattctttat
      121 ctttaatttt taaaacttaa tgctaagtaa acctgtttat ttcataaaac taatatatat
      181 tctatgctct attttccttt ttattctata tttaaatttt ttatttacaa gaatattttt
      241 aatataacat atttgataaa attaatttac atttctttca cttgtaattt ttgcaatctt
      301 gtattagatt aatattaaaa ataatttatc aataacctta ttcacaaaga cagtttttat
      361 atatcaaaat ataatataaa tactatgcta cattttagga ggattttatt atgagcaaaa
      421 aagtattaat aatgtctgct tccactggtg gtgggcataa tagggctgct ctagctatta
      481 aagaagaact tacttccaaa acacttgatg gagagccaat tgaatgtgaa ataatagata
      541 gtttgaaatt agttaataat acaatggata aaataatttc aagaggatac gaaaaatctg
      601 ctatatatac tcccaaagcc tatggaagtg tatatagatt atctgaaaca aatcttttgt
      661 caaaaaatga atttaaagat aatctactaa taacattcat ggctaaaaaa tttaaaaagc
      721 ttattagaag cgaaaaacca gacttgatta taggaacgca tccttttcca atgattgctc
      781 taagtacatt gaaaaaaaac tttaatttac ataataatga atcaaatgct tacacagagc
      841 atttttataa acattataca aataccatta atgttcctcc gcttatttct gtattaactg
      901 attatactac tcattctaca tggatacaaa atgaaattga ttactatatt gttggtcatg
      961 aatatgtaaa agaattattg gtgtttgatg gagttgaacc gagtaaaatt agaacatttg
     1021 gaatccctgt tgaaaaatct tttctctctc acagagataa agatattgtt ctttctgagt
     1081 tgaatttatc tcctgataaa ttaactgttt tacttatggg gggtagcttt ggggctggaa
     1141 atataaaaga gacacttgat gaattactgg atactgatag agactttcaa atacttgtaa
     1201 ttactggtaa gaatgaaagc ctaaaagaaa aaatcgaaaa aaaacttatg tctcgttatc
     1261 atgataaaaa tgtatgtgtt cttggataca ctgataagat gaatgatatt ttagcttcta
     1321 tagatgtatt agtaagcaaa cctggtggac ttactacaac tgaggctctt ttaaaagatg
     1381 tacctatgat agttccttat tatataccag gtcaggaaga agagaatctt gatttcttat
     1441 ctaattgtgg tgcagcactt agaactacaa aaaaatataa cttgtcaata ttactaaaag
     1501 ttttaataga tgaccctagc agattagaaa tgctaaaaag aaatattaaa tcaataagaa
     1561 agtcaaattc agcccaaaat atagctaatt taattttaaa tataatttct ggttgataaa
     1621 ataaataaaa tccctatagt atatatatta tctattaggg attttttatt ttctaattat
     1681 ttaatattaa attggttcat aaaagtcttc ttcataatca tactgataat ttttataatt
     1741 atttcta
//
LOCUS       DBGJFF010000280         1743 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.285, whole genome shotgun sequence.
ACCESSION   DBGJFF010000280 DBGJFF010000000
VERSION     DBGJFF010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.285"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            207..1547
                     /locus_tag="ACOIAF_003900"
     CDS             207..1547
                     /locus_tag="ACOIAF_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HGZ2670845.1"
                     /translation="MIKSSLFWNIFIAISFMIEWFLCKKMLDYTSEKRISNKKINFCV
                     VGIIVFADIIYFFDVPPNIRVIISMIITIAFYKISYDVGFIKSVLISLIYWMLLIATD
                     ALSISLTVWLNSINDMSSMGTGDIYRIESIILGKILLIVSLLLYRALKIKLNIEINKR
                     TLFYIVIPIVANIASFFVIFEYIFNPGSGSLIHNFQFVMISVVLLISSITLVLIAKKL
                     QQYNTLVVENNMIKNTLKYENEYYEDIEKKYLKTRLLSHDMKNHILCINAMIKKGIDV
                     TSYLNNLQVEIQSNDLLFNTGNFILDAILSEKKEVCDKNDIELKIGINFSCCEAVELV
                     DICHIFSNILDNAIEACNKIDSGNRVICVKGDVMQNFYLIRVENTKINKIITKNGDIL
                     TDKKGSLHGLGIKSVKNSVNKYGGNVVIEYDDKKFVVKIAIPLRNVKKAVNHVI"
BASE COUNT          698 a          127 c          277 g          641 t
ORIGIN      
        1 taaatttata tagttattat taaaaaaagc aataaaaagt ttaattcaat ttagcaaatt
       61 tagtgatata attaaaaaag aaaagaaata tggacttatt tgaaataatt ttgacaatct
      121 atttactaca cgtgtagagt cgtatataat taaattatac ataaaatata aaaaaataat
      181 ttagtaatac gttaggagaa agtattatga taaagtcaag cttattttgg aatattttca
      241 ttgcgatatc ctttatgata gaatggtttc tttgtaagaa aatgctagat tatacaagtg
      301 agaaaaggat ttctaacaaa aaaataaact tttgtgtggt aggtattatt gtttttgctg
      361 acataatata tttttttgat gtacctccaa atataagagt aattatatct atgataatta
      421 ctattgcatt ttataaaatc tcatatgatg tggggtttat aaagtctgta ttaatatcct
      481 taatttattg gatgttacta atcgccacag atgctttgag tatatcttta acagtttggc
      541 ttaattctat aaatgacatg agtagtatgg ggacgggaga tatatacaga attgagtcta
      601 taatattggg aaagatactt ttgattgtgt ctttgctttt atatagagcc ttaaagataa
      661 aacttaatat agaaataaat aaaaggacat tattttatat agttattcct atagtcgcta
      721 atatagcatc tttttttgta atttttgaat acatatttaa tccagggtct ggtagcttaa
      781 tacataattt ccaattcgtt atgatttctg ttgtattgct tatatcaagt atcactttag
      841 ttttaattgc taaaaaatta cagcaatata atactttagt tgtagaaaac aatatgatta
      901 aaaatacttt gaagtatgaa aatgagtatt atgaagatat agaaaagaaa tatttaaaaa
      961 ctagattact aagtcatgat atgaaaaatc acatattgtg tataaatgct atgattaaaa
     1021 aaggtattga tgttacttct tatttaaata atttgcaggt agaaattcag agtaatgact
     1081 tgttatttaa tactggtaat tttatattag atgctatatt aagtgaaaag aaagaggttt
     1141 gtgataagaa tgatatagaa ctaaaaatag gaataaattt tagttgttgt gaagctgttg
     1201 aattagtgga tatatgtcat atattttcaa atattttaga caatgcaatt gaggcatgta
     1261 ataaaattga tagtggaaat agagttattt gtgttaaagg tgatgttatg caaaattttt
     1321 atttaataag agttgaaaat actaagataa ataagattat tactaaaaat ggagatattt
     1381 tgacagacaa aaaaggttct cttcatggac ttggaataaa aagtgtaaaa aactctgtaa
     1441 ataagtatgg tggtaatgtt gtaattgaat atgatgataa aaaatttgta gttaaaattg
     1501 ctattccact tagaaatgtg aagaaagcag ttaatcatgt gatttaaatg tatataaatt
     1561 tgatgtgtag aaatttaata tataagaatt aatgtataaa agtgatgcat agaagtttaa
     1621 tatataagaa ttaatgtata aaagtgatgc atagaagttt aatatataag aactaatgta
     1681 taaaagtgat gcataaaagt ttaatatata agaatttaat atgtataaaa gtgatgcata
     1741 aaa
//
LOCUS       DBGJFF010000281         1683 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.286, whole genome shotgun sequence.
ACCESSION   DBGJFF010000281 DBGJFF010000000
VERSION     DBGJFF010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.286"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            76..423
                     /locus_tag="ACOIAF_003901"
     CDS             76..423
                     /locus_tag="ACOIAF_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HGZ2670846.1"
                     /translation="MHENKKNLLDGSLAQYNDTAVPKIPVFAGNDITSEVYYFKPNQV
                     LNAHRHPNGEQIFVFLKGEGKMKLGEHECDVKNGDTVFVPTGEWHEITNGSNEEMVAV
                     QITKINAGAEYRG"
     gene            complement(573..1622)
                     /locus_tag="ACOIAF_003902"
     CDS             complement(573..1622)
                     /locus_tag="ACOIAF_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGZ2670847.1"
                     /translation="MFKKIVAIASSIIVSTICLTGCDFNSNDKKDESSSSKNLDKVTI
                     AEVTHSVFYAPQYAAITKGFFEDEGIKIDLINTQGADKTMAALISGEANVGLMGPEAS
                     IYVYNQNKNDYAVNFAQLTKRDGSFLVARKKMPNFSYNDLKGTEILGGRKGGVPLMTF
                     EYVLKEKGLTIGENLKAGNVNVRTDVQFGVMAGAFAGGEGDFTTAFEPTATAMEKEGS
                     GYIVTSIGKDSGEIPFTAYSTTRSYMKNNKDLIQRFTNAVYKGQLWIQQASSKEVAKA
                     MQPFFEDISLEDLTTVVDRYKSIDAWSQNPVLKEEGLKKLMTVMKEAKELDKEAPYKD
                     IVNTEFANNAIKNIK"
BASE COUNT          562 a          271 c          236 g          614 t
ORIGIN      
        1 tgtaaagtga gaatactatt ttcttatatt ggtataataa tagtaaatct aatatagaaa
       61 agaggtattt ctagtatgca tgaaaataaa aaaaatctat tagacggaag cttagctcaa
      121 tataatgata cagcagttcc aaaaatacca gtatttgctg gtaacgatat aacttcagaa
      181 gtttattatt ttaaacctaa tcaagttctt aatgctcata gacaccctaa tggtgaacaa
      241 atatttgtgt tcctaaaagg agaaggtaaa atgaaacttg gtgagcatga atgtgatgta
      301 aaaaacggcg atactgtatt tgtaccaaca ggagagtggc atgaaattac taatggaagc
      361 aatgaagaaa tggtagccgt tcaaattaca aaaattaatg ctggagcaga atacagagga
      421 tagatatata agtacagtta gtactttaat atctatttta ttatataaac tttaatctca
      481 aatataaaaa gagtctttta aaaggctctt tttatatttt tgttaatttt cattataaaa
      541 acaagaaaat atctagctaa aactaatata ttttatttaa tattttttat agcattgttt
      601 gcaaactcag tatttacgat atctttataa ggtgcttcct tatctagttc tttagcttct
      661 ttcataacag tcatcaattt cttcaaacct tcctctttta atacaggatt ttgagaccat
      721 gcatctattg atttatatct atctacaact gttgttaaat cttctaaaga tatatcttca
      781 aagaaaggtt gcatagcctt agctacttct ttgctagatg cttgttgaat ccataattga
      841 cctttataaa cagcatttgt aaatctttga attaaatctt tattgttttt catgtatgat
      901 ctcgtagtag agtatgctgt aaaaggtatt tctccagaat cttttcctat agatgtaact
      961 atatatccac taccttcttt ttccattgct gttgctgtag gttcaaatgc tgttgtaaag
     1021 tcaccctctc cacctgcaaa agctccagcc attacaccaa attgtacatc agttcttaca
     1081 ttaacattgc ctgcttttag attttctcca atagtaagac ctttttcttt tagaacatat
     1141 tcaaaagtca ttagtggaac tccacctttt ctaccaccta atatttcggt tccttttaaa
     1201 tcattgtaag agaaattagg catttttttc ctagcaacta gaaaactacc atctcttttt
     1261 gtcaattgag caaagttcac tgcataatcg tttttgtttt gattatatac atatatagat
     1321 gcttcaggac ccattaaacc tacgtttgct tcacctgaaa tcaaagctgc catagtttta
     1381 tctgcacctt gggtatttat aaggtcaatc tttatacctt catcttcaaa gaatcctttc
     1441 gtaatagcag catactgtgg agcataaaaa acagagtgag taacttctgc tatagttact
     1501 ttatccagat ttttagacga agaagattca tcttttttgt cattagagtt aaaatcacat
     1561 ccagttagac atattgtact tacaatgata gagcttgcta ttgcgactat ttttttaaac
     1621 ataataaata cctcccataa gacatacgaa tagtatatag tattaaatat ttattgtagt
     1681 tgt
//
LOCUS       DBGJFF010000282         1676 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.287, whole genome shotgun sequence.
ACCESSION   DBGJFF010000282 DBGJFF010000000
VERSION     DBGJFF010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.287"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(<1..517)
                     /locus_tag="ACOIAF_003903"
     CDS             complement(<1..517)
                     /locus_tag="ACOIAF_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HGZ2670848.1"
                     /translation="MMNNELNTIILETLNNADITSNDIPSIDLYMDQIISLIDNKLSA
                     NKRFESDKILTKTMINNYSKEGLIKPVKGKKYTKEQILQMIIIYSMKNTLTIQEIKRI
                     LHGVYEKDNFSEKDLVSCYEKFMLIKENQRKNIPDFIESNFENISINPENKDDLLIAL
                     LSLTSMADQLKN"
     gene            678..1331
                     /locus_tag="ACOIAF_003904"
     CDS             678..1331
                     /locus_tag="ACOIAF_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HGZ2670849.1"
                     /translation="MYQFFLKGRDPISSLTHFIGACLSLLATIILVFQSVTLQETSLL
                     MIVSVSVFGLSLIALYSASSYYHFLKGTPEQELFFRKVDHAMIYVLIAGSYTPICLNF
                     MEKKEGIIFVTAIWIVAFIGIIIKIFWMDAPRWLSTSIYLLMGWAIVFDINAFNSIPK
                     DCLRLLIMEGVSYSIGAIIYIIKKPNISPEFGFHEIFHIFIMIGSLFHFLAVLLYVL"
     gene            complement(1489..>1676)
                     /locus_tag="ACOIAF_003905"
     CDS             complement(1489..>1676)
                     /locus_tag="ACOIAF_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670850.1"
                     /translation="DDFMYKYDDYVNLTNDLDLPVEIRNKCVRIVSVSENLEDIIIVH
                     KFKLYVVNEKYIKGIME"
BASE COUNT          570 a          213 c          209 g          684 t
ORIGIN      
        1 tattttttaa ttggtcagcc attgaagtca gacttaataa ggctatcaat aaatcatctt
       61 tattttctgg atttatacta atattttcaa aattagactc tataaagtcc ggtatattct
      121 ttctttgatt ttcttttatt aacatgaatt tttcgtaaca tgaaactaaa tctttttcac
      181 tgaaattatc cttttcatac acaccatgta agattctttt aatttcttgt attgtaagtg
      241 tatttttcat agaataaata attatcatct gtaaaatttg ttctttagta tacttttttc
      301 ccttaactgg tttgattaat ccctctttgc tgtaattatt tatcatggtt ttagtaagaa
      361 ttttatcact ttcaaatctt ttattagcag aaagcttatt gtcaattaga gaaataattt
      421 gatccatata taagtcaata gatggaatat cgtttgaagt tatatctgca ttatttagtg
      481 tttctagtat tatagtatta agttcattat tcatcatatg ttacctccat aatatatata
      541 atacactaaa ttagaaagta aaacaatatt aaggcttgta aaaaaaacat aaagtggtat
      601 aattaaatat ataatatagt aatcaaaact atatttatga atctgatttt taaaatacat
      661 aattgtagga ggacgaaatg tatcaatttt ttttaaaggg tagagaccca ataagcagtt
      721 taactcattt tataggtgct tgtttatctc ttttagcaac tataatttta gtttttcaat
      781 ctgttacatt acaggaaact tctctcttga tgatagtatc tgtatcggta tttggacttt
      841 cactaattgc tttatatagt gcaagttcgt attatcattt tttaaagggg acaccagaac
      901 aagaattgtt ttttagaaaa gtagaccatg caatgattta cgtattgatt gctggctctt
      961 atacaccaat atgtctgaat tttatggaaa aaaaagaagg cataatattt gttacagcaa
     1021 tatggatagt tgcatttatt ggtataataa taaaaatatt ttggatggat gcacctagat
     1081 ggttatctac cagtatatat ttacttatgg gatgggcaat tgtttttgat ataaatgcat
     1141 tcaattcaat tcctaaggat tgccttagac tattaattat ggaaggtgta tcttactcta
     1201 taggtgcaat tatttatata attaaaaagc ctaatataag tcctgagttt ggatttcatg
     1261 aaatattcca tatttttatt atgataggtt ctttatttca ctttttagca gttttattgt
     1321 atgttttata gttttaaaag atttgattaa ttgatactaa aattctacaa tgaataaaat
     1381 ggaactgcct tatatagatt atatcttcga caataagaca gttccttatt ataaatatta
     1441 aattctactt ataataaaat tttgataaaa atagtccatt caaaatgcct attccataat
     1501 tcctttaatg tacttttcat ttacgacata tagtttgaat ttatgtacta taattatatc
     1561 ctctaaattc tctgaaacac taacaattct gacacatttg tttctaattt ctacaggtaa
     1621 atctaaatca tttgttaaat tcacatagtc atcatattta tacataaaat catccc
//
LOCUS       DBGJFF010000283         1642 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.288, whole genome shotgun sequence.
ACCESSION   DBGJFF010000283 DBGJFF010000000
VERSION     DBGJFF010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.288"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            206..427
                     /locus_tag="ACOIAF_003906"
     CDS             206..427
                     /locus_tag="ACOIAF_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HGZ2670851.1"
                     /translation="MGYLKEDKQDYCCEFARPYINRQIYDDTYNPKTALKRGTLFPEL
                     DLIESNNYNDWLYANPKNRVRAKKGGNMY"
     gene            427..690
                     /locus_tag="ACOIAF_003907"
     CDS             427..690
                     /locus_tag="ACOIAF_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HGZ2670852.1"
                     /translation="MSLNRRELLERISEYQFACIELNLYLDNNPRDKKALDSYNRYCD
                     KFTQAVCDYESKYGALTNFGYESSEYPWSWISEPWPWDKSFYK"
     gene            752..1324
                     /locus_tag="ACOIAF_003908"
     CDS             752..1324
                     /locus_tag="ACOIAF_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HGZ2670853.1"
                     /translation="MWIYQKTLEHPVNIRQADPRMAKYIMTQLGGPNGELAAATRYLQ
                     QRYTMPTGKSRALLTDIGTEEMAHVEIISSVLYQLIGNCTPEELKAAGLGSNYANFGH
                     GLQPVDSNGVNFTTSYINVFGDSVTDLHEDMAAEQKALATYYQLINLTDDPDLKDILR
                     FLGEREVVHYQRFGEALMDVYEFTECKHQF"
BASE COUNT          660 a          177 c          299 g          506 t
ORIGIN      
        1 taaactctat attatttatt taaaatagat atttatgaaa gattaattag aaaaatttta
       61 gaggtaaaat aagaaaaaaa gatacttatt taatcattta atcttattaa agacctatta
      121 aaactaccag ttataatata gaaccagtat acaattttta aaaataatgc tattttaaaa
      181 ttctaaaaat taaggaggat aattaatggg atatttaaaa gaggataaac aagactattg
      241 ttgtgaattt gctagaccat acatcaatcg tcaaatttat gatgacacat acaatccaaa
      301 aacagcatta aaaagaggta ctttatttcc tgaattagat ttaatagagt caaataacta
      361 taatgattgg ctatatgcaa atcctaaaaa tagagttaga gctaaaaaag ggggtaacat
      421 gtactaatgt cattaaatag aagagaactt ttagagagaa tttcagaata tcaatttgcc
      481 tgtatagaat taaatttata tttagataac aatccaaggg ataagaaagc tcttgattca
      541 tacaacagat actgcgataa atttacacaa gctgtgtgtg attatgaatc taaatatgga
      601 gcattaacta attttggata tgaatcaagt gaatatcctt ggtcatggat atctgaacct
      661 tggccatggg acaaatcatt ttataaataa aattattaaa ctttaaaatt agatttatta
      721 ttaaatatat attaaggagg agtaagataa tatgtggatt tatcaaaaaa cactggaaca
      781 tccagttaac ataagacaag cagaccctag aatggcaaaa tatatcatga ctcagttggg
      841 aggacctaat ggtgagttgg cagctgcaac tagatatctt caacaaagat atactatgcc
      901 aactggaaaa tctcgtgcac ttttaactga tataggtaca gaggaaatgg ctcatgttga
      961 gataatttct tcagtgttat atcaattaat aggcaattgt actccagaag agcttaaggc
     1021 tgctggactt ggtagtaatt atgctaattt tggacatggt cttcagccag tagattctaa
     1081 tggagtaaac tttactacaa gttatattaa tgtctttggc gattcggtaa ctgatttaca
     1141 tgaggatatg gctgctgaac aaaaagcatt ggctacgtac tatcaattaa taaatttaac
     1201 tgatgaccct gatttgaaag atatattgag atttttgggt gagagggaag tagttcacta
     1261 tcaaagattt ggtgaagcat taatggatgt ttatgagttt acagagtgca agcatcagtt
     1321 ttaatgaata attaaaagtg aatggagagt aagcatcagt tttaataaag aattaaaagt
     1381 gaacagagag taagcataag ttctaatgaa gaattaaaag tgaatggaga ataagcatca
     1441 tttttaatga agaattaaaa gtgaatggag aataagcatc atttttaatg aagaattaaa
     1501 agtgaaaaga gagtaagcat catttttaat gaagaattaa aagtgaatgg agagtaagca
     1561 tcatttttaa tgaagaatta aaagtgaatg gagagtaagc atagttctaa tgaagaatta
     1621 aaagcgaatg gagagtaagc at
//
LOCUS       DBGJFF010000284         1640 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.289, whole genome shotgun sequence.
ACCESSION   DBGJFF010000284 DBGJFF010000000
VERSION     DBGJFF010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.289"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            312..779
                     /locus_tag="ACOIAF_003909"
     CDS             312..779
                     /locus_tag="ACOIAF_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGZ2670854.1"
                     /translation="MKFLNVLIVVEDIEKSKKFYYDVLGLKVICDFGENVVLEGNISL
                     QEKKLWLEFINKSDSEVKFNGNDAELYFEEDNFDTFVERLSTMKDIDYVHLAIEHRWG
                     QRAIRFYDLDGHIIEVGETMSSVCRRFLDSGLSIDEVAKRMDVTVEYIESVLE"
BASE COUNT          668 a           96 c          241 g          635 t
ORIGIN      
        1 ttaatattat tagtgtatat taatattccc ttgaaattaa aaaaacatag atatttcagt
       61 aatctaaaat agtctacaaa aataatagtt ttatcaaaat taataagaat acatatagat
      121 ttaaacctaa aagtatgaac atgtctgttt tttacaagat taaaatttat attatatgat
      181 aaaattaaat taacttaact atttcaataa gacttataat ttttttcttt atactagacc
      241 ttattgaaac ctttatagtt taattctaat tatatattat aaataaagta tgtgtatatt
      301 aaggaggtaa tatgaagttt ttaaatgttt taatagtagt tgaagatatt gagaaatcaa
      361 aaaagtttta ttatgatgtt ttggggttaa aggtaatctg tgattttgga gaaaatgttg
      421 tattagaagg taatatatca ttacaagaaa aaaagctttg gttagaattt attaataaga
      481 gtgacagtga agttaagttc aatggaaatg atgctgagtt atattttgag gaagataatt
      541 ttgatacttt tgttgaaaga ttgagtacaa tgaaagatat agattatgtt catctagcta
      601 tagagcatag gtggggtcaa agagcaatta gattttatga tttagatggg catattattg
      661 aggttggaga aactatgtca tctgtgtgta gacgtttttt agatagtggt ctttcaatag
      721 atgaggtagc taaaagaatg gatgtaactg tcgaatatat tgaatcagtt ttagaataga
      781 tggattatat taaagtaaaa ctatattttg tatagaagta agggctgtaa gtaaacttgt
      841 atggaaacaa atttacttac agtttttgtt tttgtaagat acagaatata atatttgtta
      901 taattttatt tggattttta aaaagtgcat gattttcctt acatattata atagaattta
      961 atatttattg gatgatttaa atttagaaaa atataagtat tagcttataa aagataatta
     1021 tacttactat caagctgtag aatcatatat actattttat gtaattatgt taaaatttaa
     1081 ttgaacaaat taaaaatata gtgaaaattg tagttttagt ttatttattt aataaatatt
     1141 tcaaataaaa taatgtttaa ataagaaaca taaataataa tattattatt gttcttaaag
     1201 acaaaaaatc ttagcttaaa aatttgtatc atataaaatc cctacatatt atggaagtaa
     1261 tgatacatta tatgatatac acaataaagt ataaaagtat ttacatgttt tttctatata
     1321 ttttataagt tttaggtatg gaaaattact aaataaaaat tagattaatg attatgatag
     1381 tatagataaa gtttattctt tttattaata gaaattgttg tataaaaata ataaaagtga
     1441 ggagagtgat atttaaataa aaaaatttta ataagtattg gtaatatagt ttttagggat
     1501 gattatttta tgtaaaaagt tgttatttgt aaagataaat atagaaaata ttataagtat
     1561 tgatttagtg ataatctaaa aggtgagaat tgtaataaaa agagaacata tccttcaaat
     1621 ggatgtaaac ttataaataa
//
LOCUS       DBGJFF010000285         1601 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.290, whole genome shotgun sequence.
ACCESSION   DBGJFF010000285 DBGJFF010000000
VERSION     DBGJFF010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.290"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            106..579
                     /locus_tag="ACOIAF_003910"
     CDS             106..579
                     /locus_tag="ACOIAF_003910"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGZ2670855.1"
                     /translation="MFKEYLIRELKEDDIKNALDLIWNVFEEFESPDYCDEGITTFHQ
                     FIEYNSIIEKIKEKTLFFWGCFDSKKLIGVLAIRDLNHICMLFVDKNYHKHGVAKKLM
                     TTGIQYCICNKFNLTEITVNSSPYAVEVYHKLGFKDTAVEQIVDGIRFTPMKLLI"
     gene            complement(938..1495)
                     /locus_tag="ACOIAF_003911"
     CDS             complement(938..1495)
                     /locus_tag="ACOIAF_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HGZ2670856.1"
                     /translation="MRVSREIVIQATSDIVNKDGLNKVSLKVIAEKLGIRTPSLYNHI
                     ESLDDLLLEVAHKGMREMNNQMIKASIGNFGDTAIKCISIAYFNFIISNPGVYETIQW
                     ATWHGNNETLEIFDEYKSLLEKLILSCNLNTKNTGEVLNLIMSVLHGYSTLQLGKSLL
                     SKEEAIKGLADSIDTVLLGIHKKYD"
BASE COUNT          590 a          206 c          173 g          632 t
ORIGIN      
        1 tatatattat cataatattc taaatttata aaacaaattg tattgaatta aatttattta
       61 agatatataa ttataattaa aataataata aaggtgttgt ttataatgtt caaagaatac
      121 ttaataagag aattaaaaga agatgatatt aaaaatgcat tagacttaat ctggaacgta
      181 tttgaagaat ttgaatctcc agattattgt gatgaaggaa ttacgacatt tcatcaattt
      241 atagaatata actctattat tgaaaaaatc aaagaaaaaa cactcttctt ttggggttgt
      301 tttgactcta aaaaacttat tggagtctta gctattagag atttaaatca tatatgtatg
      361 ttatttgtag ataaaaacta tcataaacat ggtgttgcaa aaaaattaat gactacaggc
      421 atacaatact gtatttgtaa taaatttaat ttaactgaaa taactgtaaa ttcatcacct
      481 tatgctgttg aagtttatca taaattagga tttaaagata ctgctgttga gcaaattgtt
      541 gacggtatac gttttacacc tatgaaattg ttaatataat aaattaaata aaaagcaaag
      601 tctcttaaat attttatagt gactttgctt ttaaaccaag atttaaaaat aatgttatct
      661 tttttatttt ttaataatat actttaaaaa cattactaat atgaactcta atttattttt
      721 gatatccttt ttgactaaca tataaattaa ttcaattatt aagcataaac tatgttattt
      781 ctcttatttg taagcaatat tggtcatttg aaaataaata ttcttaaaat atactgttct
      841 cctgtggatt ttcctttggt ataaacgata aaattttaat ttacatgcct tgtaaagaag
      901 tgttcaataa ataagttaaa ataatttatt tacctcctta atcatatttt ttatgaatac
      961 ctaataacac agtatcaata gaatcggcta aaccttttat agcttcttct ttactcaaaa
     1021 gtgatttccc aagttgcaaa gtcgaataac catgaagtac actcattatt aagtttaata
     1081 cttcacctgt attctttgta tttagattac aagaaagaat aagtttttct aataatgatt
     1141 tatattcatc aaagatttct aatgtttcat tatttccatg ccaagtagcc cactgaattg
     1201 tttcataaac accaggatta gaaattataa aattaaagta tgctatactt atacacttta
     1261 tagctgtatc tccaaaattt ccaatagacg cctttatcat ttgattattc atctctctca
     1321 ttcctttatg agctacttca agtaataaat catctagact ttctatatga ttgtacaaag
     1381 atggtgtacg tattcctagc ttttctgcaa taacttttaa agaaactttg ttaagcccat
     1441 ctttattaac tatatctgat gtagcttgta taactatttc tctactaaca cgcatgattt
     1501 tccctctttc atttaaaatt aattacttta ttagtttagc taatctaatt agtttcgtca
     1561 attattgtaa tttataaaat tctttatact attataaaat c
//
LOCUS       DBGJFF010000286         1568 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.291, whole genome shotgun sequence.
ACCESSION   DBGJFF010000286 DBGJFF010000000
VERSION     DBGJFF010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.291"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            <1..1356
                     /locus_tag="ACOIAF_003912"
     CDS             <1..1356
                     /locus_tag="ACOIAF_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HGZ2670857.1"
                     /translation="HSIYNQIKRGDIEGVKIGAGTHKRLANVPFRITSKTTGENHVVV
                     TDDNGQFSTSAEWASHKHNTNAGKTSEDGVWFGTSEPDDSKGALPYDTYIIEELRSES
                     NKGFELIPPFEIVVSRNNLVVDLGTLTDEYEKEISIHTTATSKDGEKTILAGKEVTIV
                     DTVKLDGLTKGTKYQLKGWQMLKEENAELIIDGKRVENDYTFVADDEEMKVEISYTFN
                     ASALGGKNLVTFEELYDFSNPDEPVKVAEHKDIEDDGQTVLITERIIKIHTTATDKDG
                     NKELEAGKKVTIIDTVTLESLEVGTQYKLVGWQMLKEENAELLINGKRVESDYTFTAD
                     SETMKVEVAFTFDATSLDGKQLVTFEELYDFSNPDEPKKVTEHKDIEDKGQTITFKEK
                     PEEPEKPETPPTPEKPNRPSDSPKTGDSTNVMAFVVMLLVSAGGLAGTYLYKRRKMKK
                     S"
     gene            1369..>1568
                     /locus_tag="ACOIAF_003913"
     CDS             1369..>1568
                     /locus_tag="ACOIAF_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670858.1"
                     /translation="MMKEKSRSPPKLSNGRLPLILACLPTTCKNTGSQGCVSIDFTLY
                     YLGDCWYFPNRQNKKSQSINLS"
BASE COUNT          532 a          309 c          360 g          367 t
ORIGIN      
        1 cattctatct ataatcagat taagcgtgga gatattgagg gtgtaaaaat cggtgcaggt
       61 acacacaagc gtcttgctaa tgttcccttt aggatcacaa gcaagacgac gggtgaaaat
      121 catgtagtgg taactgatga taacgggcaa ttctccacat ctgctgaatg ggcttctcat
      181 aagcacaata caaacgcagg aaagaccagc gaggacggtg tgtggtttgg aacttctgaa
      241 ccagacgaca gcaagggtgc gttaccttat gatacttaca tcattgaaga attacgctct
      301 gaaagcaaca agggttttga gcttatccca ccttttgaaa tcgtggtatc aagaaataac
      361 cttgtggttg atttagggac tttgactgat gaatacgaaa aagaaatctc tatccatacc
      421 acagcgacca gcaaggacgg cgaaaagact atccttgcag gaaaagaggt aacaatcgtt
      481 gatactgtca aattagacgg acttacaaaa ggcacaaagt atcagttaaa aggctggcag
      541 atgttaaagg aagaaaacgc cgagcttatc attgacggga aacgtgtaga aaacgattat
      601 acctttgtcg ctgatgatga agaaatgaaa gtggaaattt cctatacatt caatgcgtct
      661 gctttaggtg gcaagaacct tgttaccttt gaagaattgt atgatttcag caatccagac
      721 gaacccgtaa aagttgcaga acacaaagac attgaggacg acgggcagac ggtacttatc
      781 acagagcgta tcatcaagat acatactacc gctaccgata aggacggcaa caaagagctt
      841 gaagcaggaa aaaaggtaac aatcattgat accgtaacct tagagagctt agaagtcggt
      901 acacagtaca aacttgtggg ctggcagatg ttgaaagaag aaaatgcaga acttcttatc
      961 aatggaaagc gtgtggaaag tgattacacc tttactgctg acagcgaaac tatgaaagtg
     1021 gaagttgctt ttacgtttga cgctacttct cttgacggca aacagcttgt aacttttgaa
     1081 gaattgtacg attttagcaa tccagacgaa ccgaagaaag ttaccgagca taaggatatt
     1141 gaggataagg gacagacgat tacctttaag gaaaagccag aagaaccaga aaaacccgaa
     1201 acaccaccga caccagaaaa gcctaacaga cctagcgaca gtcccaaaac gggcgacagt
     1261 acaaatgtaa tggcatttgt cgtgatgttg cttgtgtctg ctggtggatt agctggaaca
     1321 tatctttaca aacgccgtaa aatgaagaaa tcataaggcg gtaacggtat gatgaaagaa
     1381 aaatcacgct ctccgccgaa gctgtcaaac ggcagacttc cgcttattct ggcttgtctg
     1441 cctacaacct gcaagaacac gggtagtcaa gggtgcgtaa gcattgactt taccctttac
     1501 tatctggggg attgctggta cttccccaac cgccagaata agaaatcaca atcaattaac
     1561 ttatcaaa
//
LOCUS       DBGJFF010000287         1551 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.292, whole genome shotgun sequence.
ACCESSION   DBGJFF010000287 DBGJFF010000000
VERSION     DBGJFF010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.292"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     misc_binding    481..744
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            895..1446
                     /locus_tag="ACOIAF_003914"
     CDS             895..1446
                     /locus_tag="ACOIAF_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan transporter"
                     /protein_id="HGZ2670859.1"
                     /translation="MKTNTKKLTLNAILLAMGLLIHQITPAIGLPMKPDVPLAMLFVI
                     LVLNRDDYKTCLIAGIVTGIFTALTSSFPGGQIPNVIDKTLTANIVFLLMNISYNMPF
                     IKKLAKKTQDTVVVAIIMPIGTLVSGTIFLLAAQAIVGLPGASFTALFLAVVLPAVLI
                     NLVAGIFLFKIVSLSIRRVSYQA"
BASE COUNT          632 a          151 c          242 g          526 t
ORIGIN      
        1 gtagtgctaa tatttattaa aagaaagaag aaaaaatttt actaaaaata attaaaagaa
       61 tatcattttt ttaatcattg gtatctattt aataatatga tgtaattaaa atggagtaat
      121 attctgtttc aaaggaggca tataaataca ggaatgagta ttaaatgatg gttaattaag
      181 taaatttatt gataaatgta cttaattaac tttgaagaat ttaattcctc atgaatcaaa
      241 atataaaatt acatcaacag ttattcacaa aattaatata agaagaatta gagccaaaga
      301 gcagactatt agaccattaa agtaattatt catagaattt tgtatatgaa aagaaatgta
      361 tagtataatt tacttttttt ataaaaaata gattacaaaa tatatttttt ataaaaaggt
      421 attgacattt tttagaaaaa agaatatcat tattttaaca acaaaattca atacaataaa
      481 ttaaatccta tgacaaggaa gagtagttaa atttaggttc agtatagaga gctgaggatg
      541 gtggaatctt agcaagaaaa aatttgatga atggccctta gaggaataaa ttgaaatcat
      601 agttttgaga gtagacttta tcgtcagtcg cacgttatag cgaataaggt atgtaagtac
      661 ctgataatga gatgtatgaa aattgaattt tatattaatt tttatataat ttaggtggca
      721 acgcggataa tctccgtcct attagtttag gacggagttt tttattttac tgaaaattta
      781 aaagatagaa ggtggtgaga aagatgttat gaatttcaaa agctaaagat gatgatatag
      841 taggttggca aaaaaataaa ttgaaaatta aaaaaatagg aggaagtaca aaaaatgaaa
      901 acaaatacaa aaaaattaac tttaaatgca atacttttag cgatgggatt attaatacat
      961 caaatcacac cagctatagg attaccaatg aagccagatg taccactagc aatgttattt
     1021 gtaatattgg ttttaaatag agatgattat aaaacttgtt tgatagcggg tatagtaaca
     1081 ggaatattta cagcacttac atcaagtttt ccgggaggtc aaattcctaa tgtaatagat
     1141 aaaactctta cagcaaatat agtattttta ttaatgaata ttagttataa tatgcctttt
     1201 ataaaaaaat tagctaagaa aacacaagat actgtagtag ttgcaataat aatgccaata
     1261 ggaactcttg taagtggaac tatcttttta ttagcagctc aagctatagt tggtcttcca
     1321 ggggcttcat ttacagcttt attcttagca gtagtattac cagctgtttt aataaatcta
     1381 gttgcaggaa tatttttatt caagattgtt agtctatcaa taagaagagt tagttatcaa
     1441 gcttaaacac gaaataatat tttactttat aaagcattat aataactaaa taacataaga
     1501 ataaagtaga ttataactat tttattctta tgttgtttaa agatttggta t
//
LOCUS       DBGJFF010000288         1548 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.293, whole genome shotgun sequence.
ACCESSION   DBGJFF010000288 DBGJFF010000000
VERSION     DBGJFF010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.293"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(<1..316)
                     /locus_tag="ACOIAF_003915"
     CDS             complement(<1..316)
                     /locus_tag="ACOIAF_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufBD protein"
                     /protein_id="HGZ2670860.1"
                     /translation="MKNLNLLINKLYSKNHNEAYKTFLFLENESLKSNITYCFFDSFL
                     EMIDNENSYIRTRGLLLISANAQWDIDNKIEINIDSILSHIVDKKPSVSRMFIKSIPN
                     ITK"
     gene            complement(522..899)
                     /locus_tag="ACOIAF_003916"
     CDS             complement(522..899)
                     /locus_tag="ACOIAF_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family (seleno)protein"
                     /protein_id="HGZ2670861.1"
                     /translation="MCDKVIASKYHEKGFNCAESVIKAYNEEFNTDIPVCLGSGLGSG
                     CGVASLCGAVNASNIIIGYVKGRNHEDESTKAKVYAKDLTTTVRKEYGSELCIDLKKD
                     LVACREIMDFAYDSLKETLKKEL"
     gene            complement(1015..1509)
                     /locus_tag="ACOIAF_003917"
     CDS             complement(1015..1509)
                     /locus_tag="ACOIAF_003917"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HGZ2670862.1"
                     /translation="MSLVYDVYKSKLGNLYILSEDGFIVSIYIGDEKFNNLKNECIKK
                     GIDIKRSKEDLKEAISQIDDYFNGKRKVFNLNIRIEGTEFQKSVYREMLKIPYGETLC
                     YSDIARNINNPKSVRAIGQASKSNKIPIIIPCHRVVGKKDIGGYMGNHSDLKEILLNL
                     ERQS"
BASE COUNT          514 a          260 c          118 g          656 t
ORIGIN      
        1 actttgtaat attaggtatg gatttaataa acattcttga tacagaaggt tttttatcta
       61 cgatatgact tagtatacta tctatattta tttcaatttt attatcaata tcccactgag
      121 catttgcaga gattaataac aatccccttg ttcgaatata tgagttttcg ttatcaatca
      181 tttctaggaa tgagtcaaaa aaacaatatg taatatttga ttttaaactt tcattttcta
      241 agaataaaaa agttttatat gcttcattat gattttttga atataattta ttaataagta
      301 aattgagatt cttcaatctt ctcatctccc tcattacaaa tatttattta aacaaagtat
      361 aacatattca aaaataaata taaataaaaa ctatattaaa taaaaagcct aaaattattt
      421 gctgtttatt tacaaataat aattctaaaa tatatttgat ttatttagta tcggctataa
      481 aaataagcta tcaaataatt tatatttgat agcttatttt cttataattc tttttttaaa
      541 gtttccttta gactatcata tgcgaaatcc attatttctc tacaagcaac taaatctttt
      601 tttaggtcta tacataactc tgaaccatac tcttttctta ctgttgtagt taaatcttta
      661 gcatatactt tagctttagt actttcatct tcgtggtttc tgccttttac atatccaatt
      721 attatatttg atgcatttac tgcaccacat aaactagcaa ccccacaccc tgaacccagt
      781 ccacttccta aacaaactgg tatatctgta ttaaattcct cattatatgc ttttataact
      841 gattctgcac aattaaatcc cttctcatga tattttgatg ctataacttt atcacacatt
      901 aaaatccccc ctcatttttg taaattaaaa taaattcctt attatttttg taaaaaatac
      961 ttctttttac tattattata tcaaatatta acaataaaaa catttctttc taagttaact
     1021 ctgtctttct aaatttaata gtatctcttt caaatcagaa tgattcccca tatatccacc
     1081 aatatctttt ttgccaacta ctctatgaca aggtattatt attggaattt tattactttt
     1141 acttgcttgt cctattgctc ttactgattt tggattgttt atatttcttg ctatatctga
     1201 ataacacaat gtttctccat aaggtatttt taacatttcc ctataaactg atttttgaaa
     1261 ttcagtccct tcaatacgaa tatttaaatt aaagactttt ctttttccat taaagtaatc
     1321 atctatctgg cttattgctt cctttaaatc ttccttactt ctctttatat caattccttt
     1381 tttaatacat tcatttttta aattattaaa tttttcatct cctatgtata tactaactat
     1441 aaaaccatct tcacttaata tgtacaaatt tcctaatttt gatttataaa catcatatac
     1501 taaactcata ttacttcccc cctttaatat cagtatacat ataatttt
//
LOCUS       DBGJFF010000289         1544 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.294, whole genome shotgun sequence.
ACCESSION   DBGJFF010000289 DBGJFF010000000
VERSION     DBGJFF010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.294"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            180..1319
                     /locus_tag="ACOIAF_003918"
     CDS             180..1319
                     /locus_tag="ACOIAF_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HGZ2670863.1"
                     /translation="MVIKLTIEEYKNKYFTGNKIVLSLNSFDNKELYYHETENILNLI
                     NEIDKLTSSKIFKTIEDKNDLALESYYSSVIEGAFSTRKIAKSIIRGKMKPSNKSEYM
                     IYNNHRALEYGLDNLDKLYSHKFIYDLHHILGENCLDSEEYEYRTEKVYVCDSKGEII
                     HIGLEPLKIYDFMSKLIDFMENSKVSNLIKSAIIHFYFVYVHPFSDGNGRTSRALSYL
                     YLIDKGYDTFKDFSISYMISKNRTKYYKAILDVENKGNNLTVFIEFMLKSIIQSINEM
                     RNMHDRKSLESILKEELFENDITLSATEEHILKYICNKDNYSMTLENYIKKNKSRYLK
                     AGIKEIELVDQLMEVFNNLEEIEILSKEKNIYKVNDKYLKMLDID"
     gene            1345..1533
                     /locus_tag="ACOIAF_003919"
     CDS             1345..1533
                     /locus_tag="ACOIAF_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670864.1"
                     /translation="MNNKKKAFLISGAINVISLIIVFSLFHYNIIENSTQNMIITVIL
                     IFIFEMIKIKIIEKYYKL"
BASE COUNT          668 a          131 c          220 g          525 t
ORIGIN      
        1 atagttgtaa acttcctgtg acatatattt taaatgctta ttcttaaatt tttgcattat
       61 ttattaaaat ttattatgct ttaacactaa attgagtttg ataattataa aacaaataat
      121 caaaaatagt gtaaaaatta atactttttg ttaatattaa tagataaaga taatctaagg
      181 tggtgattaa actgacaata gaagaataca aaaacaaata ctttactgga aataaaatcg
      241 tactttctct aaatagtttt gataataaag aactatacta tcatgaaact gaaaatatat
      301 taaatttaat aaatgaaata gataaattaa catcaagtaa gatatttaaa acaatagaag
      361 ataaaaatga tttggcacta gagtcttatt attctagtgt aatagaaggt gctttttcaa
      421 ctagaaagat tgcaaaatct ataattagag gaaagatgaa accaagtaat aaaagtgaat
      481 atatgattta caataatcat agagccttag aatatggatt agacaactta gataaattat
      541 atagtcacaa atttatatat gatttgcatc atatattggg cgaaaactgc ttggattcag
      601 aagaatatga gtatagaact gaaaaagtat atgtatgtga ttcaaaaggt gagattatac
      661 atatagggct tgaacctttg aagatatatg actttatgag taaattaata gattttatgg
      721 aaaatagcaa agtaagtaat ttaattaaaa gtgctataat tcatttttac tttgtatatg
      781 ttcatccttt tagtgatggt aatggaagaa catcaagagc cttatcatat ctatatttga
      841 ttgataaagg atatgatact ttcaaggatt tttcaatatc atatatgata tctaaaaaca
      901 gaactaagta ttataaggct atacttgatg tagaaaacaa aggaaataat ttaactgtat
      961 ttatagaatt tatgctcaag tctataatac aatctattaa tgagatgcgt aatatgcatg
     1021 atagaaaatc tttagaaagt atattaaaag aagaattgtt tgaaaatgac ataactctat
     1081 cagcaacaga agaacatata ttaaaatata tttgcaataa agataactat tctatgacat
     1141 tagaaaatta tataaaaaag aataaaagta gatacttaaa ggctggaata aaggaaattg
     1201 aattagttga ccagttgatg gaagtgttta ataatcttga agagatagag attttgtcta
     1261 aagaaaaaaa tatatataaa gttaatgata agtacttaaa aatgcttgat atagactaat
     1321 ttagataatc tggagaggga atatatgaat aataagaaaa aggcattttt gatttctggg
     1381 gctataaacg taatttcatt aattattgtt ttttcattgt ttcattataa tattatagaa
     1441 aatagtacac aaaatatgat aataactgta atcttaattt ttatttttga aatgataaaa
     1501 ataaaaatca tagagaaata ctataaacta taaacaagaa atat
//
LOCUS       DBGJFF010000290         1506 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.295, whole genome shotgun sequence.
ACCESSION   DBGJFF010000290 DBGJFF010000000
VERSION     DBGJFF010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.295"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            <1..533
                     /locus_tag="ACOIAF_003920"
     CDS             <1..533
                     /locus_tag="ACOIAF_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HGZ2670865.1"
                     /translation="GEFIENPKYLKKSLNKLAKLQRELSRKTIGSLNRNKARLKVARL
                     QEHIANQRKDFLQKLSTKLIKENDIICIEDLQVKNMIKNHKLSRSISDVSWSEFIRQL
                     EYKANWHGRQIVKVGKFFASSQICNKCGYKNEEVKNLNIREWICPSCNETHDRDINAS
                     INILKEGLRLITIQNK"
     gene            709..1464
                     /locus_tag="ACOIAF_003921"
     CDS             709..1464
                     /locus_tag="ACOIAF_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1835 domain-containing protein"
                     /protein_id="HGZ2670866.1"
                     /translation="MAHICFEDICYLLLKEAMKLKLIPHNKIVLFEDDLSIGNIFDVN
                     NYELRENIFKIRNVFENNPKLLFKPDLLEDRKLSKCRNIIIWCANNPKDLCGVYYVVS
                     KFQDKNIKIALVDEKKTEDALIRYSFTSEMSPEDFPFFLDKTLEIGFNEKLEFKNKWD
                     VLVKENSIFRALVGNEIKSVEETYFDKYILRNISDKWQPLIEVVANYMFEDEMRVKDW
                     FILWRLEKMASLKIVKIKGSKGDFYSDKEIRKV"
BASE COUNT          639 a          129 c          268 g          470 t
ORIGIN      
        1 gtggagaatt tatagaaaat cctaagtatc ttaagaagtc attaaacaaa cttgctaaat
       61 tacaaagaga attatcgaga aaaacaatcg gtagtttaaa tagaaataaa gcaagattaa
      121 aagttgcaag acttcaagaa catatagcta atcaaagaaa ggattttcta caaaaactat
      181 ctactaaatt aataaaagag aatgatatta tttgcataga agatttacaa gtaaagaata
      241 tgattaaaaa tcataaacta tctcgctcaa tttctgatgt gtcatggtca gaatttattc
      301 gtcaattaga atacaaggct aattggcatg gtagacaaat tgtaaaggta ggtaaattct
      361 ttgcaagttc acaaatatgc aataaatgtg ggtataaaaa cgaggaagtg aagaacttaa
      421 atataagaga atggatttgt cctagttgta atgaaactca cgatagagat ataaatgcaa
      481 gtataaatat attaaaagaa ggattaagac taataacaat tcaaaataaa taacgtatgt
      541 aagaaccgta ggaactacgg ggatagcctg tggagaatta gtaagacata ttttagtatg
      601 caaaattctg tgaagcagga accctgtgac tttagtcatg ggaggttcag aattacagtt
      661 tatatattat aataaaataa attgttaatt ttaaaagagg tgaattttat ggcacatata
      721 tgctttgagg atatttgtta tttgttatta aaagaagcaa tgaagttaaa gttaatacca
      781 cataataaaa ttgtattatt tgaagatgat ttatcaatag gtaatatttt tgatgtaaat
      841 aactacgaat taagagaaaa tatttttaag attagaaatg tttttgaaaa taatccgaaa
      901 ctattattca agccagattt gttagaagat aggaaattat caaaatgtag aaatataata
      961 atatggtgtg caaataaccc aaaagatttg tgtggagtat attatgtagt gagtaagttt
     1021 caagataaaa atataaagat tgcattagta gatgaaaaaa agacagaaga tgctctaatt
     1081 agatattcat ttaccagtga aatgtctcca gaagattttc cattcttttt agataagaca
     1141 ctagaaattg gctttaatga aaaactagaa tttaaaaata aatgggatgt gttagttaaa
     1201 gaaaattcta tttttagagc attagtaggt aacgaaatta aaagtgttga agaaacttat
     1261 tttgataaat atatattaag aaatatcagt gacaaatggc aacctttaat tgaagtggtt
     1321 gcaaactata tgtttgaaga tgaaatgcgt gtaaaagatt ggtttatact ttggagatta
     1381 gagaagatgg caagtttaaa aatagtaaaa ataaaaggaa gtaaagggga tttttattct
     1441 gataaggaga taagaaaagt ttaaaaattg aatttataat aaattgtatt aaataatggg
     1501 tataac
//
LOCUS       DBGJFF010000291         1455 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.296, whole genome shotgun sequence.
ACCESSION   DBGJFF010000291 DBGJFF010000000
VERSION     DBGJFF010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.296"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            <1..1356
                     /locus_tag="ACOIAF_003922"
     CDS             <1..1356
                     /locus_tag="ACOIAF_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HGZ2670867.1"
                     /translation="HSIYNQIKRGDIEGVKIGAGTHKRLADVPFRITSKTTGESHVVV
                     TDDNGQFSTSAEWTSHKHNTNAGKTSEDGVWFGTSEADDSKGALPYDTYIIEEMRCDS
                     NKGFELIPPFEIVVSRNNLVIDLGTLTDEYEKEISIHTTATSKDSEKTILAGKEVTIV
                     DTVKLDGLTKGTKYQLKGWQMLKEENAELIIDGKRVENDYTFIADDEEMKVEISYTFN
                     ASELGGKNLVTFEELYDLSNPDEPVKVAEHKDIEDDGQTVLITERIIKIHTTATDKDG
                     NKEIEAGKDVTIIDTVTLEGLEVGTQYKLVGWQMLKEENAELLINGKRVESDYTFTAD
                     SETMKAEVAFTFDATSLDGKQLVTFEELYDLSNPDEPKKVTEHKDIEDEGQTITFNEK
                     PEVPEKPETPQTPEKPNRPSDSPKTGDSTNVMAFLVMLLTSAGGLAGTYLYKRRTMKK
                     S"
BASE COUNT          515 a          283 c          329 g          328 t
ORIGIN      
        1 cactctatct acaatcagat taagcgtggg gatattgagg gagtgaaaat cggtgcaggc
       61 acacacaagc gtcttgctga tgttcccttt aggatcacaa gcaagacgac gggagaaagt
      121 catgttgtag ttactgatga taacgggcaa ttctccactt ctgctgaatg gacttctcat
      181 aagcacaata ctaacgcagg caagaccagc gaggacggtg tgtggtttgg aacttctgaa
      241 gcagacgaca gcaaaggtgc attgccttat gatacttaca tcattgaaga aatgcgttgc
      301 gatagtaaca aaggctttga acttatccca ccttttgaaa tcgtggtatc aagaaataac
      361 cttgtgattg atttagggac tttgactgat gaatacgaaa aagaaatctc tatccatacc
      421 acagcgacca gcaaagacag cgaaaagact atccttgcag gaaaagaggt aacaatcgtc
      481 gatactgtca aattagacgg gcttacaaaa ggtacaaaat atcagctcaa aggctggcag
      541 atgttaaagg aagaaaatgc agagcttatc attgacggaa aacgtgtgga aaatgactat
      601 acttttatcg ctgatgatga agaaatgaaa gtggaaattt cttatacatt caatgcgtcc
      661 gagcttggcg gtaaaaatct ggtaacattc gaggaattat acgatttaag caatccagac
      721 gaacccgtga aagttgcgga acacaaagac attgaggacg acggacaaac tgtattgatt
      781 acagaacgta tcatcaagat acatacgact gctaccgaca aagacggcaa caaagagatt
      841 gaagctggaa aagacgttac aatcattgat accgtaacct tagagggctt ggaagtcggt
      901 acacagtaca aacttgtagg ctggcagatg ttgaaagaag aaaatgcaga acttcttatc
      961 aatgggaaac gtgtggaaag tgattacacc tttaccgctg acagcgaaac tatgaaagcg
     1021 gaagttgcct ttacctttga cgctacttcc cttgacggca aacagcttgt aacttttgaa
     1081 gaattgtatg atttaagcaa tccagacgag ccgaagaaag ttaccgagca taaggatatt
     1141 gaggacgagg gacagacgat tacttttaat gaaaaaccag aagtcccaga gaaacccgaa
     1201 acaccgcaga caccagaaaa gccaaacaga cctagcgaca gtcctaaaac gggcgacagc
     1261 acaaatgtca tggcatttct tgtgatgttg cttacatctg ctggtggact ggctggaaca
     1321 tatctttaca aacgccgtac aatgaaaaaa tcataaggcg gtaacggtat gatgaaagaa
     1381 aaatcacgtt ctccgccgaa actgtcaaac ggcagacaag ccagaataaa aaattacaat
     1441 cataaactca tcaaa
//
LOCUS       DBGJFF010000292         1446 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.297, whole genome shotgun sequence.
ACCESSION   DBGJFF010000292 DBGJFF010000000
VERSION     DBGJFF010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1446
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.297"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            145..1254
                     /locus_tag="ACOIAF_003923"
     CDS             145..1254
                     /locus_tag="ACOIAF_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2670868.1"
                     /translation="MSEVILKNISKLYSNGFNAVKNINIDIKDKEFIVLVGPSGCGKS
                     TTLRMIAGLEEISEGELYIGDKLVNDIEPKDRDIAMVFQNYALYPHLSVYENMAFALK
                     LRKFPKDEIDKKVKEAAKILDLLPLLNKKPKTLSGGQRQRVALGRAIVRNPKVFLMDE
                     PLSNLDAKLRTAMRTEITKLHQQLGTTFIYVTHDQVEAMTMADRIVVMKDGVVQQIAT
                     PQDVYDYPANIFVAGFIGAPQMNFIDVILIEENDEIYAQNEHIKLKLNKEKHYDLIKD
                     NYINNEVVIGIRPEDIHVEDTFIKSNPDTCFKSRIEIKELMGAETYAHLKLGENTITI
                     RFDSKNRINVGDDLTLSVDNSRVHIFDKETTLAIR"
BASE COUNT          604 a          184 c          214 g          444 t
ORIGIN      
        1 aaatatattt tctaaattat aataaatctt aatataaaaa ctaactatac atattaaaca
       61 aatacattta gtcattttac acatttaata taataaaaaa atttgatact atataattaa
      121 aatttaaata tcaaggagga tattatgtca gaagtaattt taaaaaatat atcaaaactt
      181 tactcaaatg gatttaacgc agtaaaaaac ataaacatag acattaaaga taaagaattt
      241 atcgttttag taggaccatc tggttgtggt aaatcgacta cacttagaat gattgctggt
      301 cttgaagaaa tctctgaagg agaactatac attggagata aacttgtcaa tgatatagaa
      361 ccaaaagata gagatatagc tatggttttt caaaactatg ctttatatcc ccacttatct
      421 gtttacgaaa acatggcatt tgcacttaaa cttagaaaat ttcctaaaga tgaaatagac
      481 aaaaaagtta aagaagctgc taaaatatta gacttactac ctcttttaaa taaaaaacct
      541 aaaacactat ctggaggaca gagacaaagg gttgccttag gacgtgcaat agttagaaat
      601 cctaaggtat tcttaatgga tgaacctcta tccaacttag atgcaaaact tagaactgct
      661 atgagaacag agataactaa gctacaccaa cagcttggaa ctacatttat ctatgtaaca
      721 cacgaccaag ttgaagctat gactatggct gatagaatcg tagtaatgaa ggatggagta
      781 gttcagcaga ttgcaactcc acaagatgta tacgattatc cagctaatat atttgttgca
      841 ggatttattg gagcaccaca gatgaatttt attgatgtaa tattaataga agaaaatgat
      901 gaaatttatg cacaaaatga acatattaag cttaaattga ataaagaaaa acactatgat
      961 ttaataaaag acaattatat taataatgaa gttgttatag gtatcagacc agaagatatt
     1021 catgtagaag atacttttat taaatctaat ccagatactt gttttaaatc aagaattgaa
     1081 ataaaagaac ttatgggagc agaaacttat gctcacttaa aactaggcga aaatacgata
     1141 acaattagat ttgacagtaa aaacagaatt aatgttggtg atgacttaac actttcagtt
     1201 gacaatagta gagtacacat atttgacaaa gaaactactt tagctataag ataagaatat
     1261 aatcttattt ttaaggagac tttatggaag agttaaaaga ttttttagaa agacagctta
     1321 ataaaaaaat aaatatctat ccatatgaaa accaaaagtt agatgctagt agccatttag
     1381 ttactttcaa taatgctatt tatgttattg attttctaga taaaaataat ctcgataatt
     1441 taaaag
//
LOCUS       DBGJFF010000293         1436 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.298, whole genome shotgun sequence.
ACCESSION   DBGJFF010000293 DBGJFF010000000
VERSION     DBGJFF010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1436
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.298"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            <1..376
                     /locus_tag="ACOIAF_003924"
     CDS             <1..376
                     /locus_tag="ACOIAF_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HGZ2670869.1"
                     /translation="RPELDIPISGGVSEFFDMTNKDIRNKYIEEILLDKYHGLIGKTV
                     IHPMQIQIVQALSTVSYEDFADALDILDSTDSKYGVSKGVLGERMNETNPHFLWAKKT
                     LILSKIYGVLNKGVDYEELLKF"
     gene            354..>1436
                     /locus_tag="ACOIAF_003925"
     CDS             354..>1436
                     /locus_tag="ACOIAF_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HGZ2670870.1"
                     /translation="MKNCLNFNCEIDIIDNPLNLELNDFLDVGIRNNSKRRFLFISKK
                     LGKHLACKPSEMDNLGKLMATIYNEKNKEESNGTVIAFAETGTAVGHSFFDYLCGDYE
                     FIHTTREQFDELENLEFLEEHSHATDQNLYFGMLNNFKFGDEIILVDDEITTANTCMN
                     IIRKIQSIYPKKKYTICSILNWVSDENLSKVTRLERELDCKIDFVYLFKGNFKFYIDE
                     DIISNYNDNYSITKLETTSSDKVNLVVNHIYVDLEDYIGNKKYVKYTGRFGINRREQF
                     RLKEIVKRESKKITMASKESLKNEKEKILFLGTEEFMYIPMLFAKEKENKYDIYYHST
                     TRSPIINIDKNNYPIKSKFMLKSFYNI"
BASE COUNT          589 a          133 c          252 g          462 t
ORIGIN      
        1 aagaccagag ttagatatac ctataagtgg tggagtatca gaattttttg atatgacgaa
       61 taaggatata agaaataagt atatagaaga aatattattg gataaatatc atggattaat
      121 tggaaagacc gtaattcatc caatgcaaat tcaaattgta caagcgctat ctacagtatc
      181 atatgaagat tttgctgatg ctttggatat actggatagt acagacagta agtatggtgt
      241 gagcaagggt gtattaggag agagaatgaa tgaaacaaat ccacattttt tatgggctaa
      301 aaaaacattg attctatcta aaatatatgg tgtattaaat aaaggagtag actatgaaga
      361 attgcttaaa ttttaattgt gagattgaca ttattgataa tccattgaat ttagaactaa
      421 atgatttttt agacgtagga ataagaaata atagcaaaag acgttttttg tttatctcaa
      481 agaaactcgg taaacattta gcatgtaagc caagtgagat ggataatttg ggaaaattaa
      541 tggctacaat ttataatgaa aaaaataagg aagaatcaaa tggtactgtt atagcttttg
      601 ctgaaactgg tacagctgtt gggcatagtt tttttgatta cttatgtgga gattatgaat
      661 ttattcatac tactagagag cagtttgacg agttagaaaa tctggaattt ttagaggaac
      721 attcccatgc aacagaccag aatttatact ttggtatgct taacaacttt aaatttggag
      781 atgagataat attggttgat gatgagatta ccacagccaa tacatgcatg aatataatta
      841 ggaaaattca aagcatttat cctaaaaaga aatacactat atgctcaata ctcaattggg
      901 taagtgatga aaatttaagt aaagttactc gtttagaaag agagctagat tgtaagatag
      961 attttgtata cttatttaaa ggaaacttta aattttatat agatgaagat attatttcaa
     1021 attacaacga taattatagt attactaaat tagagactac ttcaagtgat aaagtaaatt
     1081 tagtagtaaa tcatatttat gttgatttag aagattatat tggaaataag aaatatgtaa
     1141 aatatacagg aagatttgga attaatagaa gagaacaatt tagattaaaa gaaatcgtta
     1201 agagagaaag taaaaaaata actatggcca gtaaagaatc tttaaaaaat gaaaaagaaa
     1261 agattctatt tttaggtact gaagaattta tgtatatacc tatgctgttt gcaaaagaaa
     1321 aagaaaataa atatgatata tactatcatt caacaacaag aagtccaatt attaatattg
     1381 ataagaataa ttatcctatt aaaagtaaat ttatgctaaa gagtttttat aatata
//
LOCUS       DBGJFF010000294         1432 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.299, whole genome shotgun sequence.
ACCESSION   DBGJFF010000294 DBGJFF010000000
VERSION     DBGJFF010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1432
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.299"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(57..1349)
                     /gene="brnQ"
                     /locus_tag="ACOIAF_003926"
     CDS             complement(57..1349)
                     /gene="brnQ"
                     /locus_tag="ACOIAF_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HGZ2670871.1"
                     /translation="MGKTKDVIVFGFALFAMFFGAGNLIFPPYLGIITGPEWLIAFLG
                     FTFADAGLALLAVMATAKFDGNVVEMFKRCGMKLGILIGCADILCIGPFLAIPRTGAT
                     TYEMGIMPLFGTSIPVLLFCILFFALSYVLTIRPSKVVDIVGQFLTPALLIALAFIII
                     KGIISPLGDIVDKPMIPNVFAEGIGQGYQTMDAFAAIALASVLIVSLNDKGYSTISDK
                     LKMIGKAGVLACGGLALVYGGLCFLGATVSTMYGTDAVQSQVIVNITEGLLGNVGKAI
                     LAVVVSLACLTTSIGLTSATGQYFSRLTKGKLSYEKIVLAVSVFSAVVASFGVGTIIK
                     IASPILSIVYPPSIVLIILAFFNEKIKNDNVYKGAVYMSLLVSILTVISSYGVAVPVV
                     NSLPLNSLGFNWVVPVIIAGIIGNFIPSKSQSNTLGTN"
BASE COUNT          507 a          272 c          183 g          470 t
ORIGIN      
        1 tttactttta gttaactcat ttatttacaa ttgattatat tttaattaaa caaaacttag
       61 tttgttccaa gtgtattaga ttgactcttt gatggtataa aattaccaat tattccagct
      121 attataactg gcaccaccca gttaaatcct aatgaattaa gaggtaaaga atttactact
      181 ggaacagcta ctccataact agatataact gttagtatac ttacaagcaa tgacatatat
      241 actgctcctt tgtatacatt atcattctta attttttcat tgaagaaagc taaaattatt
      301 aaaacaattg atggtggata aactatactt aatataggag atgctatctt tattatagtt
      361 ccaacaccaa aagatgctac tactgcactg aatacagata cagctaatac tattttttca
      421 taactcaact taccttttgt aagtctagag aaatattgac ctgtggcaga tgtaagacct
      481 atagatgtag ttaaacatgc taatgatact actactgcta aaatcgcttt cccaacattt
      541 ccaagcaatc cttcagttat atttactatt acttgtgatt gtactgcatc tgttccatac
      601 attgttgata cagttgctcc aaggaaacat aatcctccat aaactagagc taatcctcca
      661 caagcaagaa cacccgcctt accaatcatt tttaacttgt cactaatagt agaatatcct
      721 ttatcattta aagatactat tagaactgat gcaagtgcta ttgctgcaaa agcatccatt
      781 gtctgatatc cttgacctat accttctgca aatacattag gtatcatagg tttatctact
      841 atatctccaa gaggtgatat aatacctttt ataattataa atgctaaagc tattaaaaga
      901 gctggagtta aaaactgtcc aactatatct acgaccttag aaggtcttat tgttagtaca
      961 tatgaaagcg caaaaaaaag tatacagaat aataacactg gaattgaagt tccaaataaa
     1021 ggcattatac ccatttcata agttgttgca cctgttcttg gtatagctaa gaatggtcct
     1081 atacataaaa tatctgcaca acctataaga atccctaact tcataccaca tcttttaaac
     1141 atttcaacta cgtttccatc gaatttagct gtagccatta ctgctagaag tgccaatcca
     1201 gcatcagcaa acgtaaatcc taagaaggca attaaccatt ctggacctgt aattattccc
     1261 aagtaaggtg gaaatattaa gttacctgct ccaaagaaca ttgcaaataa cgcaaatcca
     1321 aatactataa catccttggt cttacccatt ataaaatccc cctttttaac cttattaaat
     1381 taataaaatg tctcgattgt ttacgcttgc ataaataagt tgaattaaac aa
//
LOCUS       DBGJFF010000295         1395 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.300, whole genome shotgun sequence.
ACCESSION   DBGJFF010000295 DBGJFF010000000
VERSION     DBGJFF010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1395
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.300"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(110..1150)
                     /locus_tag="ACOIAF_003927"
     CDS             complement(110..1150)
                     /locus_tag="ACOIAF_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HGZ2670872.1"
                     /translation="MDKHSDSVMYGGMEVYNIWGTTTVDSIIGESDVMRALKNRICKI
                     GNSDSTVAITGESGTGKELIARAIHLAGNRGNKTFVAINCAAIPEPLLESELFGYTKG
                     AFSGADSKGKIGKFELANGGVVFLDEIGDMPIQLQSKLLRVLQEKKFTRIGSNELIDI
                     DVRIISATNRDLFELVKENKFREDLYYRLNVIPLEVPPLRERGDDIQILINNFIDKYS
                     RKMKKNVYHIEPDVEQMLLKYPWPGNIRELENTIELALNLLEEDGIIHKGIINRKILE
                     YFKLNNINIKLLEESDYEISMEQDILTLEEFERAYIKKVLKFYGNDTKSKKLAAKKLG
                     ISLATLYRKIDV"
BASE COUNT          443 a          255 c          138 g          559 t
ORIGIN      
        1 gataataaaa tgctattaca caaaaaaaca cttagaaaat agttttccaa gtgtttttta
       61 tcaattttat aatttcaaat tttagaattg agaataaaaa gcctaattat tatacatcta
      121 tttttctgta caatgtagct agtgatattc caagtttttt ggcagccagt tttttagact
      181 tagtatcatt tccgtaaaac tttaacactt tctttatata ggctctttca aactcttcaa
      241 gtgtaagtat gtcttgttcc atacttatct catagtcact ttcctcaagt aactttatat
      301 ttatattatt taacttaaaa tattcaagta tctttctatt tattatgcct ttatgtatta
      361 ttccatcttc ctcaagaagg tttaatgcca gttctatagt attttccaac tctctaatat
      421 ttcctggcca aggatactta agcaacattt gttccacatc tggctctata tggtacacgt
      481 ttttcttcat ctttctcgaa tatttgtcta taaaattgtt tattaatatt tgtatatcgt
      541 ctcctctttc tctaagtgga ggcacttcta aaggtattac attcaatcta tagtataagt
      601 cttctctaaa tttattttcc ttaaccaact caaataaatc tctattagta gctgatatga
      661 ttctaacatc tatatcaatt agttcatttg aacctattct cgtaaacttc ttttcctgta
      721 ataccctcaa taactttgat tgtagttgaa ttggcatatc tcctatctca tctaaaaata
      781 caacccctcc attggccaat tcaaacttac caattttacc ttttgaatct gctccactaa
      841 aagctccttt tgtatatcca aaaagttcac tttctagtaa aggttctggt atagcagcac
      901 agtttatagc aacgaatgtt ttattccctc tgtttcctgc gagatgaatg gctcttgcta
      961 ttaattcttt tccagtgcca ctttctccag ttatagctac agtagaatca ctatttccaa
     1021 ttttacaaat tctattctta agagcacgca ttacatcaga ctctccaatt atggagtcta
     1081 ctgttgttgt tccccaaata ttatatactt ccattccacc atacattact gaatcactgt
     1141 gtttgtccat aaaatatcaa ctccaaataa gtattctata taataatcat tcacattatc
     1201 tatttaatta tatcataaca gaaaagctta gttataattt aataatatta ttttaaaaca
     1261 aaacttaata aaaagtatat ctacttagtt ttaatttgat agatatactt tcttcatttt
     1321 acaaaaatcc tctattaaat aagcaattac aatgccatat tttatatgga aataattttt
     1381 aaaattttta attta
//
LOCUS       DBGJFF010000296         1370 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.301, whole genome shotgun sequence.
ACCESSION   DBGJFF010000296 DBGJFF010000000
VERSION     DBGJFF010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1370
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.301"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            223..1239
                     /gene="gap"
                     /locus_tag="ACOIAF_003928"
     CDS             223..1239
                     /gene="gap"
                     /locus_tag="ACOIAF_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HGZ2670873.1"
                     /translation="MKIKVGMNGFGRIGRAVLRIAQEELGDNIEIVAINARATTESLA
                     HLFTYDSCYGTFRGEVEAKDEDTLVVNSKEIKILRYNDPEELPWKELGVDIVIESTGL
                     FTQREKAEKHIKAGAKKVIITAPGKNEDITIVIGVNEKEYDNEKHNIISNASCTTNCL
                     APFAKVLDEKFGIVKGLMTTVHSYTNDQRILDKSHKDLRRARAAAESIIPTTTGAAKA
                     VSRVLPQLEGKLNGFSLRVPTPTVSLVDLVCELKENVTSEQVNSVLKDAAEGELKGVL
                     GYCDKPLVSIDYKGDSRSSIIDALSTMVIEDNMVKVVSWYDNEWGYSSRTVDLVKYVA
                     EKLK"
BASE COUNT          582 a          153 c          246 g          389 t
ORIGIN      
        1 gtcccaaggg acattgagta aaataaatga tttcagctta tttacactaa aaagtacata
       61 ctaaatgagg ggtaaaataa agaaaaatga tatactaatt gtcttttgat aataaaaaaa
      121 catggaattt gattatttaa tcaagaaaaa gtattgaaca aatgatttat ttagtgtaca
      181 ataatcatgt aaaaacatag taatggaggg agacatttca taatgaaaat taaggtagga
      241 atgaacggat ttggaagaat aggaagagct gtattaagaa tagctcaaga agaattagga
      301 gataatatag aaattgtagc cataaatgca agagcaacaa cagaatcttt ggcacattta
      361 tttacatatg attcatgcta tggaactttt agaggtgaag tagaggcaaa ggatgaagac
      421 actctagtag taaactctaa agagataaaa atattaagat ataatgaccc agaagaatta
      481 ccttggaaag aattgggggt tgatatagta atagaatcaa caggattatt tacacaaaga
      541 gaaaaggcag aaaaacatat taaagcagga gcaaaaaaag taattataac agctccagga
      601 aaaaatgaag atataactat agttatcgga gttaatgaga aagaatatga taatgaaaag
      661 cacaatataa tttcaaatgc atcttgtact acaaattgtc tagctccttt tgctaaagta
      721 ttggacgaaa aatttggtat agtaaaaggg ttaatgacta cagtacattc atatactaac
      781 gatcaaagaa tattagataa aagccataaa gatttgagaa gagcaagagc agcggctgaa
      841 tctataatac ctaccactac aggagcagca aaagctgtgt caagagtact accacaatta
      901 gaaggaaaat taaatggttt ttcacttaga gtaccaacac caacagtttc acttgttgat
      961 ttagtgtgcg aacttaaaga aaacgttaca tctgagcaag ttaactctgt gttaaaagat
     1021 gctgctgaag gagaattaaa aggtgtttta ggatattgtg acaagccact tgtttctata
     1081 gattataaag gagattcaag atcatctatt atagacgctc tatccacaat ggttatagaa
     1141 gacaatatgg ttaaggtagt ttcttggtat gataatgaat ggggatattc ttcaagaact
     1201 gttgacttag taaaatatgt agctgaaaaa ttaaaataat atatatttct taaagcaaat
     1261 tttaaaaaaa ttttttaaaa aaattatata aaactaaaaa aaaagattat gaaaaatatt
     1321 gaaggaaaaa ttttttcata atcttttttc tttcatgcaa aaagatacaa
//
LOCUS       DBGJFF010000297         1359 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.302, whole genome shotgun sequence.
ACCESSION   DBGJFF010000297 DBGJFF010000000
VERSION     DBGJFF010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1359
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.302"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            88..1026
                     /locus_tag="ACOIAF_003929"
     CDS             88..1026
                     /locus_tag="ACOIAF_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HGZ2670874.1"
                     /translation="MNKRMKLIPYEINENLRGAKNKFPYGIKQMNARGMWDEGYTGKN
                     IVVGIIDTGCDISHPLLKGKIIGGANFSDDSNGNKNIYEDFNGHGTHVAGIIAASNYN
                     NEVMGVAPDCKLLIAKALNKDGTGTYQSIINAINFAVNNKVDIISMSLGGNKDDKNLK
                     NAVMQAVKNNISVVCAAGNNGDGDSSTSEYSYPASYAEVIEVGAINENYLVEKFSNSN
                     TTIDLVAPGRNIISTYMDNKLAIMSGTSMSAPYVSGSLALIKEWAREEFERDLDEAEL
                     YAQLIKCTRALGIPRTEQGNGYLYLNLYKYKNNSKR"
BASE COUNT          557 a          121 c          253 g          428 t
ORIGIN      
        1 gatataggtt ttaatttgat ttataaacct attataaaat aaaattcatg atttttataa
       61 gcaataaaat ttttaggagg atatatgatg aataaaagaa tgaaactaat tccatatgaa
      121 ataaatgaaa atctaagagg tgcaaaaaat aaattcccat atggaataaa acaaatgaat
      181 gctaggggaa tgtgggatga aggttatact ggtaaaaata ttgtagttgg tataatagat
      241 acaggttgtg atatatctca tcctctttta aaaggaaaaa taattggtgg tgcaaatttt
      301 agtgatgaca gtaatggaaa taaaaatata tatgaggatt ttaatggtca tggaactcat
      361 gtggcgggta ttatagctgc atctaactat aataatgaag ttatgggagt agctccagat
      421 tgtaaattat taatagcaaa agcattaaat aaagatggta ccggaacata tcaaagtata
      481 attaatgcta ttaactttgc tgtaaataac aaggttgata ttatatctat gtctcttggg
      541 ggaaacaaag atgataagaa tttaaaaaat gctgtcatgc aagcagtaaa aaataatatt
      601 tctgtagtgt gtgcagcagg taataatgga gatggtgatt ctagtacaag tgagtatagt
      661 tatccagcca gttatgctga ggtaatagaa gtaggtgcaa taaatgaaaa ctatttggtt
      721 gaaaagttta gtaattcaaa tactacaata gatttggtgg ctccaggaag aaatattata
      781 tcgacttata tggataataa acttgctatt atgagtggta ctagtatgag tgcaccatac
      841 gtatcaggct cattagcact aattaaagaa tgggcaagag aggagtttga aagagattta
      901 gatgaagctg aactgtatgc acaattaata aaatgtacga gagcgcttgg aatacctaga
      961 acggaacaag gaaatggata cttatattta aatctttata aatacaagaa taatagcaaa
     1021 agataatttt tgatttgatg ataaaatagc tatattatat agagtcgaga caaataataa
     1081 aaattactta ggtgtaagat tttacatcaa tgtataaagg tattaagtga taaaatttat
     1141 aaacatatta gctagttaga attgaaaaat aaatatacga tattatagat agcacatctg
     1201 gaaaaggtgt gtttttctat gtacttatac atataaaaga tttataagag atgcaaaagt
     1261 actataagtt agaaattttt ctatgaaaga ttagaaatac gatacagtgt tttgatatta
     1321 taaaatgaat ataaaatata aatgataaat attatcaat
//
LOCUS       DBGJFF010000298         1325 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.304, whole genome shotgun sequence.
ACCESSION   DBGJFF010000298 DBGJFF010000000
VERSION     DBGJFF010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.304"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            202..1191
                     /locus_tag="ACOIAF_003930"
     CDS             202..1191
                     /locus_tag="ACOIAF_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HGZ2670875.1"
                     /translation="MLKEEKTYQEKDAKVFNGVAALIMILLFLVVSIGIMVFGIVKLD
                     SGFTGIGSLMLILGLVFIVVDFILFFGLKMINPKEAIVLVLFGNYYGTIKKEGYYWVN
                     PFCSAINPAVSRINVSKSSSDEKVDISTNSRGKKVSLKTMTLNNEKQKVNDLLGNPII
                     IGVVVIWKVIDATKAVFNVDNYNTFLSIQCDSTIRNVSRLYPYDVSEDGDEKSLRGSS
                     QEIADRLKDELQSRVDIAGIEVCEVRITHLSYAPEIAAAMLQRQQAEAIIAARKKIVE
                     GAVSMVEMALTNLSENNVVTLDEERKAAMVSNLLVVLCGNKDAQPVVNSSSIY"
BASE COUNT          498 a          127 c          243 g          457 t
ORIGIN      
        1 ataccatatt catatatcat atgatttatt tggaaatcaa tattaataca ttaaaataac
       61 taaatacatg tatgcataaa gatgtataaa aaatatataa acatatagta ttaattttaa
      121 attcaacaat ttaattgtta aaagtgttat tattaaatta gagatatctt aacttaatat
      181 actgagatgg gagtgataga tatgttaaaa gaagaaaaaa cttatcaaga gaaagacgca
      241 aaagtattca atggagtggc agctttgata atgattttat tatttttagt agtttcaata
      301 ggtatcatgg tttttgggat agtaaaactt gatagtggat ttactggaat aggttcactt
      361 atgttaattt tgggacttgt atttatagta gtagatttta tcctattttt tgggctaaaa
      421 atgattaatc ctaaagaagc tatagttcta gttttgtttg gtaactatta tggaactata
      481 aaaaaagaag ggtactattg ggtgaatcca ttttgttcag caataaaccc agcagtatca
      541 agaattaatg tatcgaaatc ttcatctgat gaaaaggtag atatatcaac taattcaagg
      601 ggcaaaaaag tatctttaaa aacaatgact cttaataatg aaaagcagaa agtcaatgat
      661 ttattaggaa atccaattat aataggagtg gttgtaattt ggaaggttat agatgcaaca
      721 aaggcagtat ttaatgtgga taattataat acattcttat ctattcagtg tgattctact
      781 attcgtaatg tttcaagact ttatccatat gatgtatctg aagatggaga tgaaaaatct
      841 cttcgtggta gtagtcagga aatagcagat agattaaaag atgaattaca atcaagggtt
      901 gatattgcag gaattgaagt gtgtgaagtg agaattactc atctttctta tgcaccagag
      961 attgcagcag caatgttaca acgtcaacaa gcagaggcta taatagcagc acgtaaaaag
     1021 attgttgagg gggctgtaag tatggtagag atggcattga ctaatttaag tgaaaataat
     1081 gttgtgactt tggacgaaga acgtaaagca gctatggtaa gtaatctgtt agttgtgctt
     1141 tgtggcaata aagatgcaca accagtggta aacagcagtt ctatttatta aattataaaa
     1201 agtttaatag tttattttat tgatatatag aaaatttagt agttcatttt attaaattat
     1261 agaaagatta atggtttgct ttattgaatt ataaaaagtt taataattta ttctattgat
     1321 atata
//
LOCUS       DBGJFF010000299         1310 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.305, whole genome shotgun sequence.
ACCESSION   DBGJFF010000299 DBGJFF010000000
VERSION     DBGJFF010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1310
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.305"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            137..622
                     /locus_tag="ACOIAF_003931"
     CDS             137..622
                     /locus_tag="ACOIAF_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HGZ2670876.1"
                     /translation="MISELMLKNFRKSSINFKTKIKLNLREKPDINSLKLKSIPEGKI
                     VKLKCVDGIWAEVESNYDKGWLLYKYLERLSNAKNNSNDTIKNRKKNYIGNIRTNGLS
                     LELRNDRTLESKVITTIPDGFKVEVCYSVGKWARVNINKNEKRYSGYVYNQYIEEHLS
                     L"
     gene            978..>1310
                     /locus_tag="ACOIAF_003932"
     CDS             978..>1310
                     /locus_tag="ACOIAF_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670877.1"
                     /translation="MNKKKKLIGIAISFLLIISIINPLQVEAEAIIESSVYISSIKEN
                     YTYDNTKFKTIKSKSRHKSRKYNKSKVKLKKRSIRTKKYGYQTINKYKRQNARRHNIL
                     TKLILSIIV"
BASE COUNT          605 a          108 c          186 g          411 t
ORIGIN      
        1 actataggga ctaaaatctt tatgtaataa aggtcatgtc agccagttgc caaaaagata
       61 ataattcttt ttgttaaaag tatttttaga gttatatatt ttaataactt aatatttggc
      121 aagaggaggg cttaagatga tatctgaatt aatgttaaaa aattttcgca aatctagcat
      181 caattttaag actaaaatta aattgaattt aagagaaaaa ccagacataa atagtttgaa
      241 attaaaaagt ataccagaag gtaaaattgt taagttaaag tgtgtagatg gaatatgggc
      301 agaagttgag agtaattatg acaaaggttg gttactttat aaatatttag aaagattaag
      361 taatgctaaa aacaatagta atgatacaat aaaaaataga aaaaagaatt acataggaaa
      421 tataagaaca aatggattaa gtttagaact tagaaatgat agaacattag aatctaaagt
      481 tataactaca atccctgatg gttttaaagt tgaagtatgt tatagcgttg gaaaatgggc
      541 aagagttaat atcaataaaa atgaaaaacg atattctgga tatgtatata atcaatacat
      601 agaagagcat ttaagtttat aatacgatac tagcaagctt taaatatatt aataaatcgc
      661 aaaaaagaat cccattaatg ggattctttt ttgtagaaac attataacag aactaaagag
      721 aataaattaa ctaatttatt tttattataa agtaattata aatggtaaat ttaattgaaa
      781 gtagatttaa cttatacttt atacaccaag tatcataaat taatactact ttgaaaaatc
      841 ttaaaaaaag taaaagttat gataataaaa ataatattaa agataataag atatataaga
      901 aacttgaaaa aatttgtaat ttttagttat atatatatta taatgaacta gttaaaaatt
      961 tttatgaggg ggaaaatatg aataagaaga aaaaattgat tgggatagct atatcttttc
     1021 tacttataat cagcattata aatccattac aagtagaagc agaagctatt attgagtcta
     1081 gtgtatacat ttcttcaata aaagagaatt atacatatga taatactaaa tttaaaacta
     1141 ttaaatctaa aagtagacat aaaagtagga aatataataa aagtaaagta aagttgaaaa
     1201 aacgaagtat aagaactaaa aagtatggat atcaaactat aaataaatat aaaagacaaa
     1261 acgcaagaag acataatatc ctaacaaaat taatattgtc cataatagtc
//
LOCUS       DBGJFF010000300         1274 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.306, whole genome shotgun sequence.
ACCESSION   DBGJFF010000300 DBGJFF010000000
VERSION     DBGJFF010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1274
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.306"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            150..860
                     /gene="rgaR"
                     /locus_tag="ACOIAF_003933"
     CDS             150..860
                     /gene="rgaR"
                     /locus_tag="ACOIAF_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgaR"
                     /protein_id="HGZ2670878.1"
                     /translation="MFRVVICDDEKIQRSILKEFTEKLLSERCLNYQILEFSCGEDLI
                     SKYPEKIDIIFLDIQMKDINGIEVAKKIRKFDEKVEIIFTTAFSEYAPQGYEVRAYRY
                     LVKPIEYSNFAMGVNLCIDNLQRKKERYIVLNSKKGFNRILIDSILYVETVKRDLVVH
                     TIGRDYKANMSMKQAEKLLCENGFFRCHTSFIVNLRRIEDLRDNMITINEQFIPISKY
                     RLKDLKVALTSLLCDIMY"
BASE COUNT          506 a          105 c          203 g          460 t
ORIGIN      
        1 tttaagttta aatattaaag agaatttcta taagtagttt aatcgacaat ttaaatttat
       61 aaagaaattt aaatttgaaa gctagtgagt acagtttgga aattgaaata aagtaaacta
      121 aatgatattt tagatttgga ggtgatatta tgttcagggt agttatatgt gatgacgaaa
      181 aaatacaaag aagcatacta aaagagttta cagaaaaact tttgtctgaa agatgtctaa
      241 attatcaaat actggaattt tcctgtggag aagatttaat atccaaatac ccagaaaaaa
      301 ttgatattat atttttagat atccaaatga aagatatcaa tggaattgag gtggctaaaa
      361 aaataagaaa gtttgatgaa aaagtagaaa ttatatttac aacggctttt tcagagtatg
      421 ccccccaagg ttatgaggtt cgtgcttata gatatttagt aaagcctata gagtatagta
      481 actttgcaat gggtgtaaat ctttgtattg ataaccttca aagaaagaag gagcgttata
      541 tagttttaaa tagtaaaaaa ggatttaata gaatactgat agattctatt ttatatgttg
      601 aaactgtaaa gagagattta gtcgtacata caataggtag agattataaa gctaatatga
      661 gcatgaaaca agcagaaaaa ttactgtgtg aaaatggctt ttttagatgt catacaagtt
      721 ttattgttaa tttaagaaga atagaagatc ttagggataa tatgattact attaatgaac
      781 aatttatacc tataagtaag tatagactta aagatttaaa agtagcatta acaagcttgt
      841 tatgtgatat aatgtattaa ttttaaaaag gtaattgaaa atatcgtaaa aaagatttaa
      901 tgtcatcaca gggctattga ttgaaactgt cttaatatat aaaatgttat ttatattaag
      961 acagttttat tatgcaaaat agcagtttat agatttttaa tatggtttaa catgtatatg
     1021 tagaggttcc attatagtag catgattatt aattagtagc ttgattatca atatactatt
     1081 attgattttt atatgcatta tatgtatttt tagaatatga ttaccttttt aaaataatga
     1141 ctatttataa aactctatag atatttgttt catttagcta cgcatacttt aattaattat
     1201 attaatattt aatggagtac aatataaata ttaaagcgac atcttatagt ttattgaaac
     1261 tatagtaatg aatt
//
LOCUS       DBGJFF010000301         1212 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.307, whole genome shotgun sequence.
ACCESSION   DBGJFF010000301 DBGJFF010000000
VERSION     DBGJFF010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.307"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            377..1057
                     /locus_tag="ACOIAF_003934"
     CDS             377..1057
                     /locus_tag="ACOIAF_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGZ2670879.1"
                     /translation="MQIDVLIIDGDKNSSQELKNFLEGKGINVYLAYNCKDAIIKIFS
                     KKFDLIFLEIILVDGDGWDFCKKIRNITTCPIIHITYINENQSILNALNSGGDDYLIK
                     PLNLEILYAKVNAILRRRDLFLNNNKNNIKLEDYNRASGVIKLENKMIKLTPTENRLL
                     NYFIENAEKTLTIKEIYEHVWMNEYLDDNYSVVVAVSGLRKKIEKDYKNPQKIITIRE
                     VGYYFNKI"
BASE COUNT          529 a           79 c          187 g          417 t
ORIGIN      
        1 taacctaatg ctcgtagaca ataatgtgga tattatcaaa gttatcttta tattaaattg
       61 ttaaagacat aattatgaga ataaaaaata tagctttgaa ttatataatt gtatatgctt
      121 atctttatac agtaaataaa aaatcaaatg aaatactaaa taaagtgtat atcttaaaat
      181 aacttactag atatacaagc atgttatgtg aattttgaag tgtgcagttt agataataaa
      241 tatatacaaa taataaagaa aatggattta taggatttaa aatttcttat aagggtaaaa
      301 aatgttatta gaaaatgtac taaaaatatt tttccaattc tttatagcaa aataatgata
      361 tttaggagag attgtaatgc aaattgatgt attgataatt gatggagata agaatagttc
      421 tcaagagctg aaaaattttt tagaaggaaa agggataaat gtctatttag catataattg
      481 taaagatgct attataaaaa tatttagcaa gaaatttgac ttaatatttt tagagataat
      541 tttggttgat ggtgatggtt gggatttttg taaaaaaatt agaaatataa ctacgtgccc
      601 aattattcat ataacttata taaatgaaaa tcaaagtatt ttaaatgctt taaattctgg
      661 tggagatgat tatttaataa agcctttaaa tttagaaata ttatatgcga aagtgaatgc
      721 tattttaaga agaagagatt tatttttaaa taataataaa aataatataa aactagaaga
      781 ttataataga gctagtggag taataaagtt agaaaataaa atgataaagt taactccaac
      841 agaaaataga ttattaaatt actttataga gaatgctgaa aagactctaa ctattaaaga
      901 aatatatgaa catgtatgga tgaatgagta tttagatgat aattatagtg tagtggttgc
      961 tgtgagtgga ttaagaaaga aaatagagaa agattataaa aatcctcaaa aaattataac
     1021 aataagagaa gtagggtatt actttaacaa gatataaagc tttataaata gttttataaa
     1081 tagaatgaat gataaggcat agataatatg ttagtatatt acaagagttt ctgtcctaac
     1141 gatatatttg taaaattaga tttagtttac ttaattaaat tatagcagta gaataagtat
     1201 aaaaaatgtc tg
//
LOCUS       DBGJFF010000302         1174 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.308, whole genome shotgun sequence.
ACCESSION   DBGJFF010000302 DBGJFF010000000
VERSION     DBGJFF010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.308"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            <1..320
                     /locus_tag="ACOIAF_003935"
     CDS             <1..320
                     /locus_tag="ACOIAF_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HGZ2670880.1"
                     /translation="KYVVPDMAQSFGTLEFAGESEPIFERDKNNRKVIARRSYNLYSD
                     VQKGENVVVEIPVQAGEKHFKYEQKVKLVNPKLYGRGYAIGDVGHTDYVLVADDIVAV
                     EEK"
     gene            336..719
                     /locus_tag="ACOIAF_003936"
     CDS             336..719
                     /locus_tag="ACOIAF_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HGZ2670881.1"
                     /translation="MRLSNGFVIDKEKTFGELKFTAVRDVFLQNEDGTPSTQLKKRIY
                     DLKCSLHGGIIPVSVPPEVPLREFPYNAVVELVNPVADTVSRKTYTGADVDWYVKAED
                     IVLKNKGNQNTGTSQNHTPQGQPKK"
     gene            821..>1174
                     /locus_tag="ACOIAF_003937"
     CDS             821..>1174
                     /locus_tag="ACOIAF_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009080624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670882.1"
                     /translation="MCEECYSDENRITPLLNPLDCLENHTQYICGTCGRCICIEHDPN
                     RGLQRWNFPFKSLEIAKLYLRTADYTTKSSCGIYEIENSKGRVSYKIFAGNEDLHLFL
                     KKNKDKKCKQMTPVFN"
BASE COUNT          418 a          185 c          272 g          299 t
ORIGIN      
        1 tgaaatatgt cgtgccagat atggcacagt cttttggaac tcttgaattt gcaggcgaaa
       61 gcgagcctat ctttgaaagg gataaaaaca accgcaaggt catagccaga cgaagctata
      121 acctttattc tgacgtacag aaaggcgaaa atgttgtggt ggaaattccc gtgcaggctg
      181 gcgaaaagca tttcaagtat gaacagaaag taaaacttgt caatccgaag ttatacggca
      241 gagggtacgc aatcggggat gtgggacata ccgattatgt attagtagct gatgatattg
      301 tagcagttga agaaaagtaa aggagtgaag aatttatgag attatcaaat ggatttgtca
      361 ttgacaaaga gaaaacattt ggagaattaa aatttacagc ggtgcgtgat gtgttcttgc
      421 aaaatgagga cgggacaccg agtacccagc ttaaaaagcg tatctatgat ttgaagtgca
      481 gtctgcatgg tggaattatc cccgtgtccg taccgccaga agtaccgtta agggaatttc
      541 cttacaatgc agtcgtggag cttgtcaatc ccgtagccga tacggtatca cgaaaaacct
      601 atacgggtgc agatgtggac tggtatgtaa aggcagaaga tattgtactg aagaataaag
      661 gcaatcagaa cacaggcact tcacagaacc atacaccgca gggacaaccg aaaaaataga
      721 cttgctgaat agattttcgt tgtagaggat atacggaact gttataatat gggtatgaag
      781 aaagcgacaa attagaattt gataaggagc gaaaagaaaa atgtgtgaag aatgttactc
      841 tgatgaaaat agaataacac cattactaaa tcccttggac tgcttagaaa accacactca
      901 atatatttgt ggaacttgtg gacgttgcat ttgtattgaa catgacccaa acagaggatt
      961 acaaagatgg aattttcctt ttaagtcatt ggaaattgca aaactttatt tacgaacagc
     1021 cgattataca acgaaaagtt catgtggtat ttatgagata gagaacagta aaggcagagt
     1081 atcttataaa atattcgccg ggaatgagga tttacatcta ttcttgaaaa agaataaaga
     1141 caagaaatgc aaacaaatga cacctgtgtt taat
//
LOCUS       DBGJFF010000303         1111 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.309, whole genome shotgun sequence.
ACCESSION   DBGJFF010000303 DBGJFF010000000
VERSION     DBGJFF010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1111
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.309"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            <1..487
                     /locus_tag="ACOIAF_003938"
     CDS             <1..487
                     /locus_tag="ACOIAF_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2670883.1"
                     /translation="TYCSISLEGKNLWILKSSPIDEREIFWSKILMNFLLNAPLSILC
                     LILVSIRLDFEIQFIIVSICLIVSFSMFVALMGLLSNLLYPNLDWKNEVAVVKRSASM
                     IVTMLVSLAYVIILGGIYVLLGISFLNVYIILASILTLILDFMIWRIISTKGVKIFKN
                     L"
     gene            590..>1111
                     /locus_tag="ACOIAF_003939"
     CDS             590..>1111
                     /locus_tag="ACOIAF_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HGZ2670884.1"
                     /translation="MRKLSKREKDFIQYGFLLFLICITTYLVLTTLDIKLIPKIIRLV
                     NKTYIFLGILTIIVYMIIESIVTHLIINAVQKTKVKFIGIKMATMGFYYNLVTPFASG
                     SQPMQIYALTKYDVSLSKSVAIVTNKTVVFQSTVTVFCGILILLNKSLLMEQVHSVKV
                     LITFGMIMNVSMLL"
BASE COUNT          408 a          105 c          170 g          428 t
ORIGIN      
        1 aacatactgt tcaatttctc ttgaaggaaa aaatttatgg atactgaaat cttctccaat
       61 agatgaaaga gaaatattct ggtcaaaaat attaatgaat tttttattga atgcaccact
      121 atctatttta tgtctaatat tagtatctat aagattagac tttgaaattc agtttattat
      181 tgtatcaatc tgtttgattg taagtttttc aatgtttgta gcattgatgg gattattatc
      241 aaatttatta tacccaaacc ttgattggaa aaatgaggtg gctgtagtaa aaagaagtgc
      301 aagtatgata gttacaatgt tagtatcatt agcttatgta attatacttg gaggtatata
      361 tgtattactt ggaatttcgt ttttaaatgt atatattatt ttggcaagta tacttacttt
      421 gatattagat tttatgatat ggagaattat ttctactaaa ggagtaaaaa tatttaaaaa
      481 tttataggtg tatttatgtc aaataaagac ttatcttttt tagtcttgca aataataggt
      541 taagtagtat actatgtata gtgatatgaa aaaatttagg agaaaataaa tgagaaaatt
      601 atccaaaaga gaaaaagatt ttattcaata tggattttta ttatttttga tatgtattac
      661 tacttattta gtattaacta ctttagatat aaaactaata cctaaaatta taaggctagt
      721 aaataagact tatatatttc ttggcatatt gacaattata gtatatatga taattgaatc
      781 tattgtaact catttgataa taaatgcagt tcaaaaaaca aaagtgaaat ttattggtat
      841 taaaatggct acaatgggat tttactataa cttagttact ccatttgcat ctgggagtca
      901 accaatgcaa atatatgcac ttacaaaata tgatgtaagc cttagtaaat cagtggctat
      961 agttactaat aaaactgtag tatttcaaag tacagttacc gtattttgtg gaattttaat
     1021 tttgctaaat aagtcattac tgatggagca agtacattct gtgaaagtct tgataacttt
     1081 tggaatgatt atgaatgtaa gtatgttgtt a
//
LOCUS       DBGJFF010000304         1098 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.310, whole genome shotgun sequence.
ACCESSION   DBGJFF010000304 DBGJFF010000000
VERSION     DBGJFF010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.310"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            <1..714
                     /locus_tag="ACOIAF_003940"
     CDS             <1..714
                     /locus_tag="ACOIAF_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670885.1"
                     /translation="VSLIILVIILSLLTAIFIFNNKNTNNKLKEVTKNNEEIVPKNLE
                     GLSIEQLIEIRDKAIDKNLEGLREFGEDSVKKRKDSILNFEFGLVAFVNKNYSNLTEK
                     EKLIRQINIVNYENSINDIMDPSGSFFELANQNYQVNEFLLKNGKDYKEWSYEGLDFL
                     IDHLEELCKFVDFDVKDVIDIIDPTIKTDLSEEQKKSLNDKLKKISSSDTLSEEVKKE
                     IKNWENNLNNLNMNKNNSN"
     gene            complement(871..1077)
                     /locus_tag="ACOIAF_003941"
     CDS             complement(871..1077)
                     /locus_tag="ACOIAF_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670886.1"
                     /translation="MSKYSSLWEYVKKNNSQSFKLTFEEIKDIAGIEIDHSFLKYKKE
                     LNEYGYQVGKISLKEKTVIFDKIG"
BASE COUNT          456 a          119 c          139 g          384 t
ORIGIN      
        1 gtaagtttaa ttattttagt aataatttta agtttattaa ctgctatatt tattttcaat
       61 aataaaaata ctaacaataa attgaaagaa gttactaaga ataatgaaga aatagtacct
      121 aaaaacttag aaggtttaag tatagaacaa ttaatagaga ttagagataa agccattgat
      181 aaaaatttag aaggtcttag ggaatttgga gaagattcag taaaaaaacg taaagattca
      241 attttaaact ttgaatttgg attagtagct tttgttaaca agaattatag caacttaaca
      301 gaaaaagaga aattaataag acaaattaat attgttaact atgaaaactc tataaatgat
      361 ataatggatc cttcaggttc attctttgaa ttagcaaatc agaattacca agtgaatgag
      421 tttctattaa aaaacggaaa agattataaa gaatggtcat atgaaggtct agattttcta
      481 attgatcatc tagaagaact gtgcaaattt gttgattttg atgtaaaaga tgtaatagat
      541 attattgatc ctacaataaa aacagacctt tcagaggaac aaaagaaatc tttgaatgat
      601 aaattaaaga aaatatcttc aagtgatact ttaagtgaag aagttaaaaa ggagataaaa
      661 aactgggaaa acaatttaaa taatttaaat atgaataaaa ataattctaa ttaattttct
      721 cataagaaaa atgcttatat cgtgataatg tgaaatgtac ataattataa acaaaaaagg
      781 ggctgtgaca ctaaaaaata gtgctacgcc cttttttatt tgtaataatc atcaatacaa
      841 attattttat tatatgtaat tatttatgtc ttaaccaatt ttatcgaaaa taacagtctt
      901 ctcttttaaa gagatttttc ctacttgata tccatattca tttaattcct ttttatattt
      961 caaaaacgaa tggtctatct ctatcccagc aatatctttt atttcttcaa aagttaattt
     1021 aaacgattga ctattgtttt tcttaacata ttcccataaa gaactatatt tactcatctt
     1081 atatcactcc ttttaaaa
//
LOCUS       DBGJFF010000305         1071 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.311, whole genome shotgun sequence.
ACCESSION   DBGJFF010000305 DBGJFF010000000
VERSION     DBGJFF010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.311"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(224..715)
                     /locus_tag="ACOIAF_003942"
     CDS             complement(224..715)
                     /locus_tag="ACOIAF_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HGZ2670887.1"
                     /translation="MGMIGCYTKISEENVFKLQQAEENLQDFVFEDANENSTINIDKA
                     WHAIHFTLTGCPFGGDDDNIFSKLVLSGNILMEIDGEFPVMLITANDVKKLSKAMNSL
                     EEQAFRKRFNINEMLENNIYPVMNDESEDDFFEYVWANLIELKKFIQEASDDGLAVIF
                     FIM"
BASE COUNT          411 a          159 c           93 g          408 t
ORIGIN      
        1 agataaataa aaatatctgc aactatatat tgcaaaaatt taaaactaat ttatatatgc
       61 taatataaat atttataaag acataagaca aatttgccta cagatttaaa tttaacttat
      121 atattttaaa aggctaatat aatatgtatt ttcttctata caatcaaaag gcttcatatc
      181 taagatatgt gatgacacta atattcacaa tttttagtaa ttatcacatt ataaaaaata
      241 ttacagctag tccatcatca gatgcttctt gtataaactt tttaagctca attagatttg
      301 cccatacata ttcaaaaaaa tcatcttcac tctcatcatt cataacagga taaatattat
      361 tttctaacat ctcattaata ttaaatcttt ttctaaatgc ttgttcttcc aaactgttca
      421 ttgcttttga tagttttttt acatcatttg ctgtaatcaa catgacagga aattctccat
      481 caatttccat aagtatgttt ccacttagta ctaatttgct aaatatatta tcatcatctc
      541 caccaaaagg acaacctgtc aaagtaaagt gaatggcatg ccatgcttta tcaatattga
      601 tagtactatt ttcgttggca tcttcaaaaa caaaatcttg taaattttct tctgcttgct
      661 gtaatttaaa aacattctct tcacttattt tagtataaca tcctatcatc cccataatta
      721 cattcctcct aagtagcgtt tttttaattg taacttataa ttaaacttag ttacaataaa
      781 atatattgag aaaagtatta tttcatctta aatttaaata cctatattat caactaaaat
      841 tctgtgttta tagcgtaagt attattattt tttcctaaaa attaaatgaa tcagaagttc
      901 tataggcaat attgactata aataaggact attactttta aaaaaaaatt taagaaatta
      961 tactgtagaa tcatgctcta cttattagct tcaaataaaa aataaatcat gttctcaaat
     1021 taaataataa ataacaataa atttaaattt taaactataa gagtttctga a
//
LOCUS       DBGJFF010000306         1050 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.312, whole genome shotgun sequence.
ACCESSION   DBGJFF010000306 DBGJFF010000000
VERSION     DBGJFF010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.312"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(109..819)
                     /locus_tag="ACOIAF_003943"
     CDS             complement(109..819)
                     /locus_tag="ACOIAF_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HGZ2670888.1"
                     /translation="MTSICICDDEKQQRNMLNKIITREMDLWGYPYQIKEYSNGESLV
                     YNLEHENYYIDIIFLDIELGKSNGVDIAKTIRKHSKDTIIIFVTGFSDYVFHGYDVGA
                     LNYILKPYKTEKICKVLKEALKKLEQWKENFITVQIGTNLCKINTKDILYFKSELRKL
                     TVVTFDKTLEYYGKLNDMETVLPSNFIRTHQRYIVNLNHVDSITQTYAKVQNEKIPIS
                     RKKYQEVVGKFTRYMLES"
BASE COUNT          364 a          145 c          112 g          429 t
ORIGIN      
        1 ctagaaatta atcttcaaaa aattaaattt gaaaaaacaa aactctaaaa actaagcttt
       61 aaaaaattaa gtttcaaaaa taaaattcta gagattgaac ttcaaaaatt aagattctaa
      121 catatacctt gtaaattttc ctacaacctc ttgatatttt ttacggctta taggaatttt
      181 ttcattctga actttggcat aagtttgagt tatactatct acatggttta gattgacaat
      241 atacctttga tgcgtacgta taaaattaga gggaagaaca gtttccatat catttaattt
      301 tccgtagtat tctaatgttt tatcaaaggt aactacagtt aattttctta gctcactttt
      361 aaagtagagg atatcttttg tattaatttt acaaagatta gttccaatct gaactgtaat
      421 aaagttttct ttccattgtt ctaatttttt tagtgcttct tttaatactt tacatatttt
      481 ttcagtttta taaggtttta agatgtagtt caaagctcct acatcatatc catgaaatac
      541 atagtcagaa aaacctgtga caaagatgat tatagtatcc tttgaatgtt ttcgtatagt
      601 ttttgctatg tcaacgccat tactttttcc aagttcaata tctaaaaaaa taatatctat
      661 gtaataattt tcgtgttcta gattgtatac tagactttct ccatttgaat attcttttat
      721 ttggtagggg tagccccaca aatccatttc tcttgtaatg attttattca gcatgtttct
      781 ttgttgtttt tcatcatcac aaatacaaat cgaagtcaat tttttatctc ctttcaaatt
      841 ttatatttat atagttaagt atataatata ttttgctacc tagatatata aaatgaaata
      901 acaattatat ccagatagca aaataaaatt ttttatatta gtatacttac acttaaaact
      961 attctattaa acttattctg aagtgctaat gattttctta ttaaaaaatc taacaatctt
     1021 tcttattata aaatacaaat gcttttatta
//
LOCUS       DBGJFF010000307         1048 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.313, whole genome shotgun sequence.
ACCESSION   DBGJFF010000307 DBGJFF010000000
VERSION     DBGJFF010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1048
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.313"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            162..866
                     /gene="cwlD"
                     /locus_tag="ACOIAF_003944"
     CDS             162..866
                     /gene="cwlD"
                     /locus_tag="ACOIAF_003944"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase CwlD"
                     /protein_id="HGZ2670889.1"
                     /translation="MRKYIKHIIFSFAMICLVVVSIFEIKNISEDVIKYMPVTNKTII
                     LDAGHGGIDPGALNKDKSTSEKDINLAITLKLRELIESSGGLVILTREDDSSLYKEEN
                     NKTTRQKYNENLKNRKEIISNSNANMFVSIHLNAFEQSKYYGAQTFYPKDKQDSKELS
                     KCIQEELKRVVDKTNNREVKPRDDIYLLKENNIPSVLIECGFLSNEKECKLLTDETYQ
                     EKIAWAIYIGIQKYLS"
BASE COUNT          468 a          109 c          158 g          313 t
ORIGIN      
        1 aacatagtga attaaatagg gacagaaata taatatcatt acataataat atattaatac
       61 caataaataa agatatatca atattaaaaa aatttaaatg tttgtactaa tatatggaat
      121 attatagtgt ttttataagt atacatatac agggtgatgt tgtgagaaag tacataaaac
      181 atataatttt tagttttgca atgatatgtc tagtagtagt atccatattt gaaattaaaa
      241 atatttctga agatgttatc aagtatatgc cagtaacaaa caaaactata attttagatg
      301 ctggtcatgg aggcattgat ccaggtgcat taaataagga taagagtaca tctgaaaagg
      361 atattaacct agcaataaca cttaagctta gagagcttat agaatcaagt ggtggccttg
      421 taatattaac tcgagaagat gacagtagcc tttataaaga agaaaataat aaaactacaa
      481 gacaaaaata taatgagaat ttgaaaaata gaaaagaaat aatatctaat tctaatgcca
      541 atatgtttgt ctctatacat cttaatgcat ttgaacaatc aaaatactat ggagctcaaa
      601 ctttttatcc taaagacaaa caagacagta aagaattatc gaagtgtatt caagaagaac
      661 ttaaaagagt agtagataag acaaacaaca gagaagtcaa accaagggat gatatatatc
      721 ttttgaaaga aaataatatt ccatcagtac tgatagaatg tggtttttta tcaaatgaaa
      781 aagagtgtaa actcttaact gatgaaacat atcaagaaaa aatagcatgg gcaatctaca
      841 taggaataca aaaatattta agttgaaata actataaaaa taggtctaaa tatgtaaata
      901 gcttgtatta aagcagttaa aatgcattaa tataggctat ttttattttg acaaaaaaat
      961 atttaaaata aaagttaaaa agttgttgac ttagaataat atagatgata ttatatataa
     1021 gtgcccaaaa ggagcaccaa aataagac
//
LOCUS       DBGJFF010000308         1038 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.314, whole genome shotgun sequence.
ACCESSION   DBGJFF010000308 DBGJFF010000000
VERSION     DBGJFF010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.314"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(2..73)
                     /locus_tag="ACOIAF_003945"
     tRNA            complement(2..73)
                     /locus_tag="ACOIAF_003945"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39..41),aa:Asn,seq:gtt)
     gene            complement(80..153)
                     /locus_tag="ACOIAF_003946"
     tRNA            complement(80..153)
                     /locus_tag="ACOIAF_003946"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(119..121),aa:Cys,seq:gca)
     gene            complement(161..236)
                     /locus_tag="ACOIAF_003947"
     tRNA            complement(161..236)
                     /locus_tag="ACOIAF_003947"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(201..203),aa:Lys,seq:ttt)
     gene            complement(245..321)
                     /locus_tag="ACOIAF_003948"
     tRNA            complement(245..321)
                     /locus_tag="ACOIAF_003948"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(285..287),aa:His,seq:gtg)
     gene            complement(329..405)
                     /locus_tag="ACOIAF_003949"
     tRNA            complement(329..405)
                     /locus_tag="ACOIAF_003949"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(369..371),aa:Pro,seq:tgg)
     gene            complement(429..505)
                     /locus_tag="ACOIAF_003950"
     tRNA            complement(429..505)
                     /locus_tag="ACOIAF_003950"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(469..471),aa:Ile,seq:cat)
     gene            complement(520..593)
                     /locus_tag="ACOIAF_003951"
     tRNA            complement(520..593)
                     /locus_tag="ACOIAF_003951"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(557..559),aa:Met,seq:cat)
     gene            complement(600..675)
                     /locus_tag="ACOIAF_003952"
     tRNA            complement(600..675)
                     /locus_tag="ACOIAF_003952"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(640..642),aa:Phe,seq:gaa)
     gene            complement(679..767)
                     /locus_tag="ACOIAF_003953"
     tRNA            complement(679..767)
                     /locus_tag="ACOIAF_003953"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(731..733),aa:Ser,seq:tga)
     gene            complement(856..931)
                     /locus_tag="ACOIAF_003954"
     tRNA            complement(856..931)
                     /locus_tag="ACOIAF_003954"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(896..898),aa:Gln,seq:ttg)
     gene            complement(939..1015)
                     /locus_tag="ACOIAF_003955"
     tRNA            complement(939..1015)
                     /locus_tag="ACOIAF_003955"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(979..981),aa:Arg,seq:tct)
BASE COUNT          279 a          278 c          233 g          248 t
ORIGIN      
        1 gctccaaggg tggggctcga acccacagcc taccggttaa cagccggttg ctccaccgtt
       61 gagctacctt ggaatatttt ggaggcgaca cccagattcg aactggggat caaggagttg
      121 cagtccactg ccttaccact tggctatgtc gccacctttt tggtgatcta tccgcgactc
      181 gaacgcggga caccctgatt aaaagtcagg tgctctaccg actgagctaa tagaccatat
      241 gaattggggt ggataatgag agtcgaactc acgacctcca gagccacaat ctggcgctct
      301 aaccaactga gctataccca ccttatattg gtcgaggtga agggactcga acccccggcc
      361 ccatggtccc aaaccatgtg cgctaccaaa ctgcgctaca cctcgaattt atattaatta
      421 atttaatgtg gtgggccatc agggactcga accccagacc taccggttat gagccgggcg
      481 ctctaaccaa ctgagctaat ggcccacatt aaattttagt agcggtaata ggagtcgaac
      541 ctatgacctt tcgggtatga accgaacgct ctagccaact aagctatacc gccatatttt
      601 ggtgcccaga ggcggaatcg aaccaccgac acggggattt tcagtcccct gctctaccga
      661 ctgagctatc tgggcatttg gcggagaggg tgggattcga acccacggcc cctttcgaag
      721 tcactggttt tcaagaccag ctccttaaac cactcggaca cctctccaca ttcggatact
      781 taaacttatt ctaaaattga acctaactat atttaaaata aaaataattc aaatatagct
      841 tagtaataca tcatttggct ggggatgcag gactcgaacc tacgcataca tgagtcaaag
      901 tcatgtgcct taccgacttg gctaatcccc aatatatgtg gtgagcgcac agggattcga
      961 accccggaca cacgccttag aagggcgttg ctctatccag ctgagctatg cacccacaat
     1021 ttatatatgt atatcaaa
//
LOCUS       DBGJFF010000309         1019 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.315, whole genome shotgun sequence.
ACCESSION   DBGJFF010000309 DBGJFF010000000
VERSION     DBGJFF010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.315"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            214..>1019
                     /locus_tag="ACOIAF_003956"
     CDS             214..>1019
                     /locus_tag="ACOIAF_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HGZ2670890.1"
                     /translation="MADCNSCPSKGNCNSQSNCSIENNPNNKFGKIIGVMSGKGGVGK
                     STVTALLANKLNKMGYKVGILDSDITGPSIPRLMGVKNVKAYSDGSYIYPVENSNNIK
                     VMSINLMIDDENEPVVWRGPLLGGVVKQFYTDVLWEELDYLLIDMPPGTGDVALTVMQ
                     SIPISGIVMVSVPQDLVSMIVSKAVNMAKKMNINVLGVIENMSYIQCPDCSKKIKLFE
                     GESTEKFLDDLDLELLGELPMTKEIIDITHNGVTEISDDLDSILTNVVEK"
BASE COUNT          372 a          111 c          180 g          356 t
ORIGIN      
        1 atatgactaa gttctataac taaattgaaa taatcacttt ttcaagaatg ttataatttt
       61 tttattgtat tctttttgct atagtctatg aataaatcaa tagaaaatct tctaagttgc
      121 ttttaaataa tgactttatg gttcaattgt actaaaatag tatatgtagg ttacaaataa
      181 tctaataaat attaaagttt ggaggtaata gatatggcag attgtaattc atgtccatca
      241 aagggaaatt gtaatagtca atcaaattgt tctattgaaa ataaccctaa caataaattt
      301 ggaaaaataa ttggtgttat gagtgggaag ggtggagttg gaaaatcaac tgtaactgct
      361 ttacttgcta ataagctaaa caaaatggga tataaggttg gtatattaga ctcagatata
      421 actggaccaa gtattcctag acttatgggt gtaaaaaatg taaaagctta ttctgatggc
      481 tcttatattt accctgtaga aaactcaaat aacataaaag taatgtctat aaaccttatg
      541 atagatgatg aaaatgaacc tgttgtttgg agaggacctt tacttggagg ggttgtaaag
      601 caattttata ctgatgtact ttgggaagaa ttagactatt tattaataga tatgcctcct
      661 ggtactggag atgttgcctt aacagttatg caatctattc ctataagtgg aatagttatg
      721 gtttcagtac ctcaagattt ggtatctatg attgtatcaa aagctgttaa tatggctaaa
      781 aaaatgaaca taaatgtcct tggagttatt gaaaatatga gttatatcca atgccctgat
      841 tgttctaaaa aaatcaagtt atttgaagga gaatctactg aaaagttttt agatgattta
      901 gacttagaac ttttaggaga acttcctatg actaaagaaa ttatagatat tactcataat
      961 ggagttacag aaattagtga tgatttagat tcaatattga ctaatgttgt tgaaaaaat
//
LOCUS       DBGJFF010000310          965 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.316, whole genome shotgun sequence.
ACCESSION   DBGJFF010000310 DBGJFF010000000
VERSION     DBGJFF010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..965
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.316"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            105..245
                     /locus_tag="ACOIAF_003957"
     CDS             105..245
                     /locus_tag="ACOIAF_003957"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670891.1"
                     /translation="MEKSNTTVTKKEKIEDIGMKLEHLLSEDITFYELLLEILYKEKE
                     EN"
     gene            complement(242..613)
                     /locus_tag="ACOIAF_003958"
     CDS             complement(242..613)
                     /locus_tag="ACOIAF_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGZ2670892.1"
                     /translation="MESLGDRIANLRKELDINQKELATKVGITEASLSRYENNLREPK
                     SEIIVRLAKALETSTDYLLGVNDNTKISKEDKLIIENLSVSEKTKKLLEKIYSLEKED
                     REAIEKMIDNAYLKRFLKEEN"
BASE COUNT          363 a          104 c          118 g          380 t
ORIGIN      
        1 atatatttta taaaatcaaa atacaattac ataaacttta ttaaaaattt gttgggaatt
       61 tctgactaac attaagaacg taagtttgtt gggggatggt atatgtggaa aagtcaaaca
      121 ctactgttac aaaaaaagaa aaaattgaag atataggaat gaaattagaa catttattga
      181 gtgaagatat tactttttat gaattattac ttgagatttt gtataaagaa aaagaagaga
      241 attaattctc ttctttaagg aatcttttta aataggcatt atcaatcatt ttttctatag
      301 cttctctatc ttctttttca agagaatata ttttttctaa gagctttttt gttttttcac
      361 ttactgataa gttctctatg attaatttat cttctttgct tatttttgta ttatcattta
      421 cgcctaataa ataatctgtt gatgtttcta atgcttttgc taatctaact attatttcac
      481 ttttaggttc tcttaaatta ttttcataac gtgacaagct agcctcagtt ataccgacct
      541 tagtagcaag ttctttttga tttatatcta attcttttcg tagatttgct attctatctc
      601 ctaaactttc catatgtaca tcactcctta ccgaaattat aagttatttt gataaaaagt
      661 aaaataaaat taccaaaaat ataaaaaaaa aaggttaaaa taataaaata tattgaaaat
      721 taccatttgt atatgtataa tatgaattat acaaatggta atttgatgag aggtggtatg
      781 ttgaaataag aaactgaatt aattaataaa tacatagaag gttttgaaaa cctttaattg
      841 agatataaac aatatgtaca aaattttttg caagtaaaat taccaaatgt ataaaataaa
      901 tcagtagcat agataatttt atgctttaat acattagatt atttaattta gttataatgg
      961 tcaaa
//
LOCUS       DBGJFF010000311          861 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.317, whole genome shotgun sequence.
ACCESSION   DBGJFF010000311 DBGJFF010000000
VERSION     DBGJFF010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..861
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.317"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(160..>861)
                     /locus_tag="ACOIAF_003959"
     CDS             complement(160..>861)
                     /locus_tag="ACOIAF_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HGZ2670893.1"
                     /translation="IICEGKKSERVILNDYINTFFYNYNFEYNIKEFENGGDLLKKYP
                     NNVDIIFMDIDLNDENGFNVSKEIKKFDESVRIIFVSSSNKYIKKGYEIGVYRYLEKP
                     LKYSLFKEAISSYLKECYKNEAILSIKCKCKIYFIKVDDILYLEVLKNDLTIHTEKGI
                     YTFKSSMKEIESNLEEHNFFRCHKSYLINLMKVNMLDIENNIVFINQNSIIVSRYKIK
                     LLREKLNSCLVDVII"
BASE COUNT          308 a          111 c           64 g          378 t
ORIGIN      
        1 taaaattgtt aatagaattg taaaataaaa tcttaatata ttcaatctgt tattgatgaa
       61 aggtatattt ttaactataa ttagttttta ttataaattt tttcatattt tacaatagat
      121 atattctatt agaatagaat ataaaggcaa aaatttacct caaattatta catctaccaa
      181 acaactattt aacttttctc tcaataattt tatcttataa cgacttacaa ttattgaatt
      241 ttgatttata aatacaatat tattttctat atctagcata tttaccttca ttaaatttat
      301 aaggtagctt ttatgacatc taaaaaaatt atgttcttct agatttgatt ctatttcttt
      361 catactactc ttaaaagtat atattccttt ttcagtatgt atagttaaat cattttttaa
      421 aacttctaaa tataaaatat catctacctt tatgaaatat atcttgcatt tacattttat
      481 agataaaata gcttcatttt tgtagcattc ttttaaatat gatgatattg cttctttaaa
      541 tagagagtat tttaagggct tctctaaata tctataaact cctatctcat aacctttctt
      601 tatgtattta tttgatgagg atacaaatat aattcttaca ctttcatcaa attttttgat
      661 ttcttttgaa acattaaatc cattttcatc atttaagtct atatccataa atatgatatc
      721 aacattatta ggatactttt ttaataaatc gcctccattt tcaaactctt tgatattgta
      781 ttcaaaattg taattataaa aaaaggtatt tatataatca tttagaatta ccctctctga
      841 ttttttacct tcgcaaataa t
//
LOCUS       DBGJFF010000312          805 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.318, whole genome shotgun sequence.
ACCESSION   DBGJFF010000312 DBGJFF010000000
VERSION     DBGJFF010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..805
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.318"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            177..422
                     /locus_tag="ACOIAF_003960"
     CDS             177..422
                     /locus_tag="ACOIAF_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670894.1"
                     /translation="MFIDEELEGYILTCKISEDFKNIPEYSDEEFYVTVYKDESSDSG
                     YYALLENKEERVVWDGEVVANNIFNNLWIVVNKVKTG"
     gene            521..784
                     /locus_tag="ACOIAF_003961"
     CDS             521..784
                     /locus_tag="ACOIAF_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1883 domain-containing protein"
                     /protein_id="HGZ2670895.1"
                     /translation="MEYSYSKMNLKKGDIVEVNLEKQANVILLDHINYVKFKNQRNYD
                     YYGGFAKKNPCRMRVPNTGTWYLVVNQDGNSGIVNFSINTIQN"
BASE COUNT          351 a           49 c          138 g          267 t
ORIGIN      
        1 tataaagaaa tatataggaa aacaaaagaa ttaattagta taatataaag ttaaaaagtt
       61 aaaatttata taatttattt ataaagtaaa gaaaaaatag aaatttgtaa taaaataaaa
      121 agtttatgac ataatatggt ataattagta aaaatataga gattggagga attgctttgt
      181 ttatagatga agaattagaa ggttatatat taacatgtaa aatatctgaa gactttaaaa
      241 atatacctga atatagtgat gaagagtttt atgttacagt ctataaagat gaaagttctg
      301 actctgggta ctatgcttta ttagaaaata aagaagaaag agttgtatgg gatggagaag
      361 ttgttgccaa taatattttt aataaccttt ggattgtagt aaataaggtt aaaactggat
      421 aatagtctaa ttatatagta tttgtaatat tttaaatgaa tttttacata tttgagatga
      481 gtaaataaca aagattattc gatactggag gagtaattca atggaatata gttatagtaa
      541 gatgaactta aaaaaagggg atattgtaga ggtaaattta gagaaacaag ccaatgtaat
      601 attgttagac catataaatt atgtgaaatt taaaaatcaa agaaattatg attactatgg
      661 tggatttgca aagaaaaatc catgtagaat gagagttcct aatactggaa catggtatct
      721 agtagtaaat caagatggaa atagtggaat agtaaacttt tcaatcaata caattcaaaa
      781 ttaattttag aatattggat attct
//
LOCUS       DBGJFF010000313          787 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.319, whole genome shotgun sequence.
ACCESSION   DBGJFF010000313 DBGJFF010000000
VERSION     DBGJFF010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.319"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(<1..>787)
                     /locus_tag="ACOIAF_003962"
     rRNA            complement(<1..>787)
                     /locus_tag="ACOIAF_003962"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          172 a          238 c          182 g          195 t
ORIGIN      
        1 cgtccatccc ggtcctctcg tactaaggac aggtcttctc aaatttccta cgcctgcgac
       61 ggatagggac cgaactgtct cacgacgttc tgaacccagc tcgcgtacca ctttaatggg
      121 cgaacagccc aacccttggg acctactaca gccccaggat gtgatgagcc gacatcgagg
      181 tgccaaacct ccccgtcgat gtggactctt gggggagata agcctgttat ccccagggta
      241 gcttttatcc gttgagcgat ggcccttcca cgcagaacca ccggatcact aagtccgatt
      301 ttcatccttg ctcgacctgt atgtcttgca atcaagctct cttttgcctt tacactctac
      361 gtacgatttc cgaccgtact gagagaacct ttgagcgcct ccgttacctt ttgggaggcg
      421 accgccccag tcaaactgcc cacctgacag tgtcccaaga ccagattcat ggcctatggt
      481 tagagtccca gtactacaag ggtggtatcc caaggatggc tccgccaaaa ctggcgtcct
      541 ggtttcaaag cctcccacct atcctgtaca tgtagtacca agacccaatg tcaagctaca
      601 gtaaagctcc atggggtctt tccgtcctgt cgcaggtatc cggcatcttc accggaatta
      661 caatttcacc gagtctgttg ttgagacagt gcccaaatcg ttacgccttt cgtgcgggtc
      721 ggaacttacc cgacaaggaa tttcgctacc ttaggaccgt tatagttacg gccgccgttt
      781 actgggg
//
LOCUS       DBGJFF010000314          780 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.321, whole genome shotgun sequence.
ACCESSION   DBGJFF010000314 DBGJFF010000000
VERSION     DBGJFF010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..780
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.321"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            71..769
                     /locus_tag="ACOIAF_003963"
     CDS             71..769
                     /locus_tag="ACOIAF_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGZ2670896.1"
                     /translation="MEKKYFEIHGVPSILWGNPSSKLYIYIHGQCGNKEEAEAFANIA
                     VPHECQVLSVDLPEHGERKGEKNSFDPWHVVPELVSIMKYAKCHWNQISLYANSIGAW
                     FSMLSFKKENLRECIFVSPILDMQQLISNMMLWANVSEEQLKHELIIPTSFGHTLSWE
                     YLQYVKQHPIIQWNVPTKILYGKHDELTEIGVVEKFVEKFNCNLTIMENGKHWFNTPQ
                     ELNSLYEWLNTLVK"
BASE COUNT          291 a          108 c          131 g          250 t
ORIGIN      
        1 tattacaaca ttattttata gtttgattaa tttaacaata actaatatat caaactgaga
       61 ggagattttt atggaaaaaa aatatttcga aattcatgga gttccttcaa tcctttgggg
      121 aaatccttca agtaaattat atatttacat acatggacaa tgtggcaata aagaggaggc
      181 tgaagctttt gctaatattg cagtcccaca tgagtgtcaa gttttaagtg ttgatttacc
      241 tgaacatgga gagcgtaaag gagaaaaaaa ttcatttgac ccatggcatg tggttccaga
      301 actagtatct attatgaaat atgcaaagtg ccactggaat caaatttcgt tatatgcaaa
      361 tagcattgga gcatggttta gtatgttaag ctttaaaaaa gagaacttaa gagagtgtat
      421 ttttgtatct ccaattctag atatgcaaca gcttatttca aatatgatgc tttgggcaaa
      481 tgtatcagaa gaacagctta aacatgaact aattatccca acttcatttg ggcatactct
      541 ttcatgggaa tatctacaat atgtaaaaca acatcctatc atccaatgga atgtaccaac
      601 aaaaatatta tatggtaaac atgatgagct tactgaaatc ggtgttgtag agaaatttgt
      661 agaaaagttt aattgcaacc ttactattat ggaaaatggc aaacattggt ttaacacacc
      721 ccaagaatta aactcattat atgaatggtt aaatacttta gttaaataaa aaataattat
//
LOCUS       DBGJFF010000315          777 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.322, whole genome shotgun sequence.
ACCESSION   DBGJFF010000315 DBGJFF010000000
VERSION     DBGJFF010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..777
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.322"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            101..712
                     /locus_tag="ACOIAF_003964"
     CDS             101..712
                     /locus_tag="ACOIAF_003964"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HGZ2670897.1"
                     /translation="MINDIKNKFKNYKPYINGYKNMKRASVLIPIVEINNTHYILFEV
                     RSKNLKHQPSEISFPGGKIESGETPYEAVIRETCEELGTFSDNIEVISSLDLLITPVK
                     FIIHPYLGYIKNINNLNINKDEVDHTFLVPIKYLLENPPNTYSNTVKVFPDENLPYGS
                     IPNQKDYKFESGSYPILFYKYKDYVIWGMTAKILENFLNFLSE"
BASE COUNT          328 a           93 c           92 g          264 t
ORIGIN      
        1 tagatagtta ctatatctta taattagata aatcataatt atatattatt gttaaaactc
       61 aattatataa tttttaataa tattatttta ggaggtaaaa atgataaacg atattaaaaa
      121 taaattcaaa aattacaaac catatataaa tggatacaag aatatgaaga gagcatctgt
      181 tttaattcca atagtagaaa taaataacac acactatata ttatttgaag ttcgctcaaa
      241 aaatttgaag catcaaccta gcgaaatttc ttttccaggt ggtaaaatag aatcaggtga
      301 gactccatat gaagctgtta ttagagaaac ttgtgaggaa cttggaactt ttagtgacaa
      361 tatagaagta atatcaagtc ttgacctcct tataacacca gtaaagttta tcatacatcc
      421 atacttagga tatataaaaa atattaataa tttaaatata aacaaggatg aagttgacca
      481 tacttttttg gttcctataa aatatctttt agagaatcct ccaaatactt atagtaatac
      541 tgtaaaagtt tttccagatg aaaatctacc atatggttca attccaaacc aaaaagatta
      601 taaatttgaa agtggaagtt atcctattct gttttataaa tataaagatt atgtaatttg
      661 gggaatgaca gcaaaaatac tagaaaactt tttaaatttt ttatcagaat aacactcata
      721 ataaatcatc aaaactttta cataagaaat ttataaaagt ttctaaattt aacaatt
//
LOCUS       DBGJFF010000316          772 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.323, whole genome shotgun sequence.
ACCESSION   DBGJFF010000316 DBGJFF010000000
VERSION     DBGJFF010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..772
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.323"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(51..257)
                     /locus_tag="ACOIAF_003965"
     CDS             complement(51..257)
                     /locus_tag="ACOIAF_003965"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGZ2670898.1"
                     /translation="MFKKKSGYTFTNFLNKVRVEKSKKYILKKDLSILDVAVLIGFNS
                     QNYYSIIFKKFNNFTPIEYKNIYN"
     gene            549..740
                     /locus_tag="ACOIAF_003966"
     CDS             549..740
                     /locus_tag="ACOIAF_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670899.1"
                     /translation="MERLMELIERKFLKDVELLELEDIENVIECKMTQPYNAHYNEYL
                     VKVKNGDEVEEYFVYLKHR"
BASE COUNT          314 a           59 c           97 g          302 t
ORIGIN      
        1 taattgtaaa aaatatgtaa aaaaataaca tctttttaga atgatatttt ttagttataa
       61 atatttttat attcaattgg tgtaaagtta ttaaactttt taaaaattat actatagtaa
      121 ttttgactat tgaatccaat tagtacagca acgtctaaaa tagacaagtc tttttttaat
      181 atatactttt tgcttttttc aactctaact ttatttaaaa aattagtaaa agtatatcca
      241 ctttttttct taaacaaaga aaaaaataac ttttattaag ttgtaattca ttacatatat
      301 tacttactgt tatatcttct gagtaatttt tgtgaatata atctatagtt ttctttacat
      361 aaaactaaga gttggtctat gtttcatttt atcaaggcat atttattaaa aaatcaattt
      421 ctatttgtta aaataattta tgaatgatgt tagaaactgt aatatattgg atataatata
      481 attaagataa actctgtatt aagaggatat atagtttatc ttaattatag gggaagtgag
      541 gatatattat ggaaagatta atggaactca tagaaagaaa atttttaaag gatgtagaac
      601 ttttagaatt agaagacata gaaaatgtaa tagaatgcaa aatgacacag ccatataatg
      661 ctcattataa tgaatattta gtaaaagtaa aaaatggaga tgaagtagaa gaatattttg
      721 tgtatttaaa acatagataa acataaaaga ggtttatatt tatttgtatg at
//
LOCUS       DBGJFF010000317          762 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.324, whole genome shotgun sequence.
ACCESSION   DBGJFF010000317 DBGJFF010000000
VERSION     DBGJFF010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..762
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.324"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            216..395
                     /locus_tag="ACOIAF_003967"
     CDS             216..395
                     /locus_tag="ACOIAF_003967"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670900.1"
                     /translation="MGNLKRQCDVSSGREKADIVLKNGTIINVFTEELITGDVAIVGD
                     TIVGIGDYKGNVEID"
BASE COUNT          310 a           93 c          148 g          211 t
ORIGIN      
        1 attcaatatg aattaaatta ttgcttaaat attaatgaga acatgggaga taatattatt
       61 cctcatgttc tctctattta attaaaggaa ttatggaaca aatttcaagt gatactttct
      121 atcaaaactc atatataata tatattaata aattaaacta taatacgtaa tacagtcagt
      181 atattgatat aaaaaactta ggaggagttg taaaaatggg taatttaaaa agacagtgtg
      241 atgtttcaag tggaagagaa aaagctgaca ttgtactaaa aaatgggaca atcataaatg
      301 tatttacaga agagttaata acaggagatg tagcaatagt aggagacact atcgttggaa
      361 ttggcgatta caagggcaat gtagaaattg attaaaaaat agctacgcac actcgggact
      421 ttagtcatga gttagtagct aaaatagtca gcatataggg aaacctatat gtagagatag
      481 gatagaacct atccaatact acttgaattg ctggaaaccc ctaaagctaa tcaaactaaa
      541 acataaggat gaaataaacc taagtgtgaa agttacgaaa gtagaaaaaa ttggttagat
      601 ggtataaggt taaaccctaa gtactttaaa atgggcaatc agcaggtaaa ctccgaatag
      661 gagaaacttc aacgactatt cctcttgagg gaagtacact acaagctatt ggtagtggaa
      721 gtgggtagac cttaacggat aatgctgaag gataagatat ag
//
LOCUS       DBGJFF010000318          674 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.326, whole genome shotgun sequence.
ACCESSION   DBGJFF010000318 DBGJFF010000000
VERSION     DBGJFF010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..674
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.326"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            <1..>674
                     /locus_tag="ACOIAF_003968"
     CDS             <1..>674
                     /locus_tag="ACOIAF_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGZ2670901.1"
                     /translation="MLVNYVLKKLERFGSLHSEGYYFDNFVNPKLVGASSIFLILLFK
                     EIIKENRLKSQAELIKNKLDMQYAHYLSIQESHMRVKKLYHDINNHIYCIDNLRNNSK
                     EINEYVNNLKDEIKTFKYIYNTGNMILDIIINEKSEVCLKKGIKFTCSINFSKVNFVK
                     PIDVSSIFSNILDNAIEACDKIVDENVNKYIRIKGTITRSFFVLKCENSKLNQLTFNK
                     NILLTN"
BASE COUNT          298 a           59 c           91 g          226 t
ORIGIN      
        1 atatgcttgt aaattatgta ttaaaaaaat tagaacggtt tgggtcatta cattcagaag
       61 gatactattt tgataatttt gttaatccaa aattggtagg agctagtagt atatttctta
      121 tactattgtt taaagaaata ataaaagaga atagattaaa atctcaagct gaattaataa
      181 aaaataagtt agatatgcaa tatgcgcatt atttaagtat tcaagaatct catatgcgag
      241 ttaagaaact atatcacgat ataaataatc acatttattg tatagacaac cttagaaaca
      301 atagcaaaga aattaatgaa tatgttaaca atttaaaaga tgaaataaaa acatttaaat
      361 atatctataa tacaggaaat atgatattag acattataat caacgaaaag agtgaagtat
      421 gtttaaaaaa aggaattaaa tttacttgct ctataaattt ttcaaaagta aattttgtaa
      481 aacctataga tgtgtcaagc atattttcta atattttgga taatgcaata gaagcatgtg
      541 ataaaatagt ggatgaaaat gttaataaat atattagaat taaaggaact ataacaaggt
      601 cattttttgt acttaaatgt gaaaatagta aactaaatca gcttactttt aataaaaata
      661 tattgttaac aaac
//
LOCUS       DBGJFF010000319          672 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.327, whole genome shotgun sequence.
ACCESSION   DBGJFF010000319 DBGJFF010000000
VERSION     DBGJFF010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..672
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.327"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            119..457
                     /locus_tag="ACOIAF_003969"
     CDS             119..457
                     /locus_tag="ACOIAF_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3784 domain-containing protein"
                     /protein_id="HGZ2670902.1"
                     /translation="MFAVGFVVIIALAFFILSVLLFNGKASMLLAGYNSMSKKEKARY
                     DKKKLCRSSSIVTFIVSIMLFIMAFLGYRIESGQMDEKKMVPFSIIFIIVILSSVFFN
                     ILYSNKKCKK"
BASE COUNT          256 a           67 c           94 g          255 t
ORIGIN      
        1 atgaattaaa gtatgataat ctatttaata aagtatgaag taaatgattt taatgtgcct
       61 ttatggtaaa attactaata agtaataaaa ttaattaaaa tatatggagg tgcttataat
      121 gtttgctgta ggatttgttg ttataattgc acttgccttt ttcattttaa gtgtactatt
      181 atttaatgga aaagccagta tgctactagc aggttataac tctatgagta agaaagaaaa
      241 agcacgatac gataaaaaaa aattatgtag aagttctagc attgttactt ttattgtctc
      301 tataatgcta tttattatgg cgtttttggg atatagaata gagagtggtc aaatggacga
      361 aaaaaaaatg gttccctttt ctataatatt tataatagtc atactttcca gtgtattttt
      421 taatatcctc tactctaaca aaaaatgtaa aaaataaata gttgtaagtt gagcagttaa
      481 ttataaaata ttgactgctt tttcatattg actactctta ttaaatattt taatatttaa
      541 taatctatgt aaaaataatg aatatagtac aaacttctaa taatgatttt acctttgtgg
      601 ttacagtcca ctatatataa tttaatatga taacagggaa ttactaatga caactaagta
      661 aacttttata aa
//
LOCUS       DBGJFF010000320          664 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.328, whole genome shotgun sequence.
ACCESSION   DBGJFF010000320 DBGJFF010000000
VERSION     DBGJFF010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.328"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(<1..>664)
                     /locus_tag="ACOIAF_003970"
     rRNA            complement(<1..>664)
                     /locus_tag="ACOIAF_003970"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          152 a          191 c          151 g          170 t
ORIGIN      
        1 gatccagccg caccttccga tacggctacc ttgttacgac ttcaccccag ttattgattc
       61 caccttcgac gacttcttcc aaaaggttag ataatcggct tcgggcgtct ccaactcccg
      121 tggtgtgacg ggcggtgtgt acaagacccg ggaacgcatt caccgcagca ttctgatctg
      181 cgattactag taactccagc ttcatgtagg cgagtttcag cctacaatcc gaactgagag
      241 tagctttaag ggattagctc caccttacgg cttggcaacc ctctgtacta cccattgtag
      301 cacgtgtgta gccctaagca taaggggcat gatgatttga cgtcatcccc accttcctcc
      361 aggttatccc tggcagtctc tctagagtgc ccaacttaat gatggcaact aaagacaagg
      421 gttgcgctcg ttgcgggact taacccaaca tctcacgaca cgagctgacg acaaccatgc
      481 accacctgtc accaatgtcc ccgaagggaa ctctccgatt aaggagatgt cattgggatg
      541 tcaagcttag gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc cgctacttgt
      601 gcgggtcccc gtcaattcct ttgagtttca ctcttgcgag cgtacttccc aggcggagta
      661 ctta
//
LOCUS       DBGJFF010000321          661 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.329, whole genome shotgun sequence.
ACCESSION   DBGJFF010000321 DBGJFF010000000
VERSION     DBGJFF010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..661
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.329"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            69..>661
                     /locus_tag="ACOIAF_003971"
     CDS             69..>661
                     /locus_tag="ACOIAF_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HGZ2670903.1"
                     /translation="MLDAFITIVSSIIEIWSCKKVFDYTSQSKTDLKKLNMYFLFSVF
                     LIISSLYINIGANSRILICIFVMFVFYKSNYKVNIYKCLVISFFYWLILIGINGLSML
                     LVATVSNLDNMNVLLSGSLYRYEAIFLGKIMLGILLYVYKASFKNFKISKKEIYICIP
                     MIANLLSFFVLYDYIINLVKISPERRTAFLVIVLLLI"
BASE COUNT          233 a           52 c           87 g          289 t
ORIGIN      
        1 aattagtatg tattttttat gatatatttt ataaaaaata taatttaatc atttttgagg
       61 atattattat gttagatgca tttataacaa ttgtttcttc tattatagaa atatggtcat
      121 gtaaaaaagt ttttgattat actagtcagt caaagaccga cttaaaaaaa ctaaatatgt
      181 attttctctt ttctgttttt ctcattattt cttctttata tataaatata ggagctaata
      241 gtagaatatt gatatgtata tttgtaatgt tcgttttcta taagtctaac tataaggtta
      301 atatatataa atgtctggta atctcatttt tttattggtt aatattaata ggtataaatg
      361 gattaagtat gttattagta gcaacagtaa gcaatttgga taatatgaat gttttactta
      421 gtggcagttt atatagatat gaagcaatat tcttgggaaa gataatgtta ggtatattac
      481 tttatgttta taaggcttct tttaaaaatt ttaaaataag taaaaaagag atatacatat
      541 gtattcctat gatagcaaat ttattgtcat tttttgtact ttatgattat attattaatc
      601 ttgtaaaaat ctctcctgaa agaagaactg cttttttagt aatagtttta ctattaatat
      661 t
//
LOCUS       DBGJFF010000322          577 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.330, whole genome shotgun sequence.
ACCESSION   DBGJFF010000322 DBGJFF010000000
VERSION     DBGJFF010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.330"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            <1..>577
                     /locus_tag="ACOIAF_003972"
     rRNA            <1..>577
                     /locus_tag="ACOIAF_003972"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          154 a          121 c          190 g          112 t
ORIGIN      
        1 caggatggac ccgcgtctga ttagctagtt ggtaaggtaa cggcttacca aggcgacgat
       61 cagtagccga cctgagaggg tgatcggcca cattggaact gagacacggt ccaaactcct
      121 acgggaggca gcagtgggga atattgcaca atgggcgaaa gcctgatgca gcaacgccgc
      181 gtgagtgatg aaggccttcg ggtcgtaaaa ctctgtcctc aaggaagata atgacggtac
      241 ttgaggagga agccccggct aactacgtgc cagcagccgc ggtaatacgt agggggctag
      301 cgttatccgg atttactggg cgtaaagggt gcgtaggcgg tctttcaagt caggagtgaa
      361 aggctacggc tcaaccgtag taagctcttg aaactgggag acttgagtgc aggagaggag
      421 agtggaattc ctagtgtagc ggtgaaatgc gtagatatta ggaggaacac cagttgcgaa
      481 ggcggctctc tggactgtaa ctgacgctga ggcacgaaag cgtggggagc aaacaggatt
      541 agataccctg gtagtccacg ctgtaaacga tgagtac
//
LOCUS       DBGJFF010000323          556 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.331, whole genome shotgun sequence.
ACCESSION   DBGJFF010000323 DBGJFF010000000
VERSION     DBGJFF010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..556
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.331"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            <1..>556
                     /locus_tag="ACOIAF_003973"
     CDS             <1..>556
                     /locus_tag="ACOIAF_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="collagen binding domain-containing protein"
                     /protein_id="HGZ2670904.1"
                     /translation="DAYKVSGDSFYMQDGKAVLPLGTLSVEETKAPNGYLLDGAYMQA
                     GDKSEQIKGLYLTQITEDGDLAVLTGSNQFSVSDKVIRGGVKIQKRDLETSDTKPQGS
                     ATLKDTAFDVISLNDNSVLVEGKLYKKNEVVKTIRTDIEGVASTSADLLPYGKFRIVE
                     SEAPDGYLTDGAKPIDFAITENGK"
BASE COUNT          170 a          106 c          137 g          143 t
ORIGIN      
        1 cggacgctta caaggtatct ggcgacagct tctatatgca ggacggcaaa gcggttcttc
       61 ctttaggaac actatctgtt gaggaaacaa aagctccaaa cggctacttg ctggacggtg
      121 catatatgca ggctggcgat aagtctgaac agataaaagg cttatacctt acacaaatta
      181 cagaggacgg cgaccttgct gtattgacgg gaagtaacca gttttccgta tcagacaagg
      241 ttatccgtgg cggtgtgaaa attcagaaaa gagatttaga aacgagcgat accaaaccac
      301 aaggaagtgc cactttgaaa gatactgctt ttgacgtcat ttccttaaat gacaattctg
      361 ttttggttga gggcaagcta tataagaaaa atgaagtggt aaagacaatt cgtacagata
      421 ttgagggtgt ggcttctact tctgctgacc ttttacctta tgggaaattc cgtatcgttg
      481 agagtgaagc tcccgacggt tacttgacag acggtgcgaa gccgattgac tttgcaatca
      541 cagagaatgg aaaaat
//
LOCUS       DBGJFF010000324          520 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.332, whole genome shotgun sequence.
ACCESSION   DBGJFF010000324 DBGJFF010000000
VERSION     DBGJFF010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.332"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(<1..356)
                     /locus_tag="ACOIAF_003974"
     CDS             complement(<1..356)
                     /locus_tag="ACOIAF_003974"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGZ2670905.1"
                     /translation="MELEITEKIEKQDEDTIFQGLLKYNLARLEDKKPVDLGIYIRDK
                     AGKIVAGFIGVTHGNWLSIKYLWVSEKLRYKGTGSQLLYKAEKIAKERGCKYVFLDTF
                     SFQAPKFYEKFGYHKV"
BASE COUNT          175 a           80 c           47 g          218 t
ORIGIN      
        1 aatactttat ggtatccaaa tttctcataa aatttaggtg cttgaaaact gaatgtatct
       61 aaaaaaacat atttacaacc tcgttcttta gcaatttttt cagccttata taaaagttga
      121 cttccagtac ctttatatct aagcttctca ctaacccata agtattttat agaaagccaa
      181 ttaccatgag taacacctat aaaacctgca acaattttac ctgctttatc tcgaatatat
      241 attcctaaat ccactggttt tttatcttca agtctagcaa gattatattt taataatcct
      301 tgaaaaatag tatcttcatc ttgtttttct attttttctg taatttctaa ttccatattt
      361 gaactttctc cttgtataat cttttgtata ttggtatttt ttaataaaat cagtagaaag
      421 aatatagttt aacattattc taataggcat ttatgctaat gtcaaactaa taatacttat
      481 tcttaatttt atattgttga tattaaattt ttttaaataa
//
LOCUS       DBGJFF010000325          509 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.333, whole genome shotgun sequence.
ACCESSION   DBGJFF010000325 DBGJFF010000000
VERSION     DBGJFF010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.333"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            36..425
                     /locus_tag="ACOIAF_003975"
     CDS             36..425
                     /locus_tag="ACOIAF_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGZ2670906.1"
                     /translation="MVLNLDFNSDKSIYVQIKEEITKAIASGELKINESLPSVRSMAE
                     NIGVNLHTVNKSYNELKEEGFLNIDRRKGAIVAQLPMKIDNTTRQKNKLDLELLVAKS
                     YLSGISKEEFLSLSSSLFDKYEVKYYE"
BASE COUNT          196 a           51 c           83 g          179 t
ORIGIN      
        1 tcgttttcat ttataaagca taatggaggt tttttatggt tttaaattta gattttaata
       61 gtgataaatc tatttatgta caaattaaag aagaaataac taaggctata gcttcaggag
      121 aattgaaaat aaatgaatca cttccatctg taagaagtat ggctgaaaat attggtgtaa
      181 atttacatac agtgaacaaa tcctacaatg aattaaaaga agagggattc ttaaatattg
      241 atagaagaaa aggtgctatt gtagctcaac taccaatgaa aatagataat acaactcgtc
      301 aaaaaaataa acttgacttg gaattattgg tggcaaaatc ttatttatca ggtattagta
      361 aagaagaatt tttgtcacta agttcaagtt tatttgacaa gtatgaggtg aaatattatg
      421 agtaagattg aatgtctatt tacaatattt ccagttctaa ttttgatgta tgctacattt
      481 tattttatac cctattttgt aggtaaaaa
//
LOCUS       DBGJFF010000326          506 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.334, whole genome shotgun sequence.
ACCESSION   DBGJFF010000326 DBGJFF010000000
VERSION     DBGJFF010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.334"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            <1..>506
                     /locus_tag="ACOIAF_003976"
     rRNA            <1..>506
                     /locus_tag="ACOIAF_003976"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          143 a          108 c          143 g          112 t
ORIGIN      
        1 tttaactgag cgttcagtta cttgacgtag acccgaaacc gggcgaccta cccatgagca
       61 ggatgaagcg aaagtaaaat ttcgtggagg tccgaaccca cgagcgttga aaagctcggg
      121 gatgacttgt gggtagcggt gaaattccaa tcgagcccgg agatagctgg ttctccccga
      181 aatagcttta gggctagcct caaggtgaga gatacggagg tagagcactg aatgtcctag
      241 ggggtattgc acctaccgaa gactatcaaa ctccgaatgc cgtcatctta tacttgggag
      301 tcagactgtg ggtgataaga ttcatagtcg aaagggcaac agcccagatc gtcagctaag
      361 gtccctaaat gtaagttaag tggtaaagga tgtgggattg cacagacaac caggatgttg
      421 gcttagaagc agccactcat tcaaagagtg cgtaatagct cactggtcga gtgatcctgc
      481 gccgaagatt tccggggcta aaactt
//
LOCUS       DBGJFF010000327          467 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.337, whole genome shotgun sequence.
ACCESSION   DBGJFF010000327 DBGJFF010000000
VERSION     DBGJFF010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.337"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(<1..333)
                     /locus_tag="ACOIAF_003977"
     CDS             complement(<1..333)
                     /locus_tag="ACOIAF_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGZ2670907.1"
                     /translation="MRMILNKGHRIRASDKNLVYRFSMGTLLFVFVAVILLLNTKQLM
                     RTDWEHFSLLDNGFTLSLYNFITILIATGVCALVAFLYYRFCYDSFKKLLHRQKLARM
                     ILENKWYEA"
BASE COUNT          142 a           90 c           95 g          140 t
ORIGIN      
        1 agcttcatac cacttgtttt ccagtatcat tcgtgccagc ttttgacggt gcagtagctt
       61 tttgaaactg tcgtaacaga agcggtaata cagaaaagcg accaatgcac aaacaccagt
      121 cgctatcagt atggttatga agttgtaaag ggaaagcgta aagccattgt ctaataagct
      181 gaaatgttcc cagtcggtac gcatgagctg ttttgtattt aatagcagga taaccgctac
      241 gaacacaaac aggagtgtcc ccattgaaaa gcgatagaca aggttcttat cgctggctct
      301 gatacggtgt cctttgttta aaatcattct cataaatcaa tcactccttt ctgggtatga
      361 aaaaagcagt caatcgtata gactaactgc tttgtgtgca tggatatgaa gttgtcaaac
      421 tggaatttaa cgttcactca tatattgttt tatttcatca gaagtca
//
LOCUS       DBGJFF010000328          446 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.339, whole genome shotgun sequence.
ACCESSION   DBGJFF010000328 DBGJFF010000000
VERSION     DBGJFF010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.339"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            164..>446
                     /locus_tag="ACOIAF_003978"
     CDS             164..>446
                     /locus_tag="ACOIAF_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670908.1"
                     /translation="MQQKCKKKALSMILSISMGLGSVASFNASNTFADTIEESTNAGN
                     AEGDTNAGNAEGDTNAGNAEGDTNAGNAGGDANAGNAGGDANAGNAGGDA"
BASE COUNT          182 a           42 c          108 g          114 t
ORIGIN      
        1 ttgaaaaaaa cagtatattc attgacaaat ataagtttta agtatatatt aaaaattatt
       61 agagtgcata taaggatgta tacagtgtat attaagaatt attagagtgt atataaggat
      121 gtatacagtg tatattaaaa attattaagg ggagggggaa tttatgcaac aaaaatgtaa
      181 aaagaaagca ctatcaatga ttttaagtat ctcaatggga cttggatcag tagcatcatt
      241 taatgcgtca aatactttcg cagatactat agaagaaagt actaatgcag gaaatgcaga
      301 aggagatact aatgcaggaa atgcagaagg agatactaat gcaggaaatg cagaaggaga
      361 tactaatgca ggaaatgcag gaggggatgc taatgcagga aatgcaggag gggatgctaa
      421 tgcaggaaat gcaggagggg atgcta
//
LOCUS       DBGJFF010000329          434 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.341, whole genome shotgun sequence.
ACCESSION   DBGJFF010000329 DBGJFF010000000
VERSION     DBGJFF010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.341"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            261..359
                     /locus_tag="ACOIAF_003979"
                     /pseudo
     CDS             261..359
                     /locus_tag="ACOIAF_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_045009167.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            361..>434
                     /locus_tag="ACOIAF_003980"
     CDS             361..>434
                     /locus_tag="ACOIAF_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGZ2670909.1"
                     /translation="MVVVEKAYKFRMYPNKKQQELINKT"
BASE COUNT          184 a           43 c           81 g          126 t
ORIGIN      
        1 cgtgaaagtg taagaagttc ataagagaac tgcataggta gtagcgaact tatgtgaacg
       61 acaacctcaa aaatgataaa agttaagact tacggttctt atatatgtta tttaaaaata
      121 aatataaaaa aacttcaaaa tacttgtttt aagtaattgt atattttaat attatgatag
      181 gttatgaaaa atggaagtta ctcatggtag aggatatgca tatagtatgt caaatataga
      241 cagccagtat tatggggaga catctttgga atcctagtta tttcgttgct actgtatcag
      301 aaaatacaga ggagcagata agaaaatata ttaataatca gaaaaaacaa gtaaagtgag
      361 gtggtagtgg tggaaaaagc ttataagttt agaatgtatc caaataaaaa acaacaagag
      421 ttaattaaca aaac
//
LOCUS       DBGJFF010000330          422 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.344, whole genome shotgun sequence.
ACCESSION   DBGJFF010000330 DBGJFF010000000
VERSION     DBGJFF010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..422
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.344"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(<1..344)
                     /locus_tag="ACOIAF_003981"
     CDS             complement(<1..344)
                     /locus_tag="ACOIAF_003981"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HGZ2670910.1"
                     /translation="MLQLIAYFSIVYCVYKASNLRGVSYVHIMTLQVFLYMAISPIPT
                     PGNVGANEVTFFTMFSNIIPKELLGYSVLLYSGFVYYFILIVSGLFTFGTHYTLNSWK
                     KEDKVNSNLDKV"
BASE COUNT          169 a           61 c           45 g          147 t
ORIGIN      
        1 ataaccttat ctaaattaga atttacttta tcttcctttt tccaagaatt taatgtataa
       61 tgcgttccaa atgtaaataa acctgaaact attaatataa aatagtatac aaacccacta
      121 tataagagta cagagtaccc caataattct tttggaatta tattggagaa catagtaaaa
      181 aatgtaacct cattagctcc aacatttcct ggtgttggaa ttggtgatat agccatatat
      241 aaaaagacct gtaatgtcat tatatgtaca tatgaaactc ctcttaaatt tgaggcttta
      301 tacacacaat aaactatact gaaatatgcg attaattgta acacagtaag taatatactt
      361 aaaactaaga ttcttacatt tttaataaat atttttatag aataattata atcatcaata
      421 ta
//
LOCUS       DBGJFF010000331          407 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.346, whole genome shotgun sequence.
ACCESSION   DBGJFF010000331 DBGJFF010000000
VERSION     DBGJFF010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.346"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            <1..>407
                     /locus_tag="ACOIAF_003982"
     rRNA            <1..>407
                     /locus_tag="ACOIAF_003982"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          121 a           80 c          116 g           90 t
ORIGIN      
        1 taagatgtgc gcactattgg atgtgcgtct aagcacttag acgtgcttga taggtaaatc
       61 cgtcaagctt agttgaggtg ttatggggag ctaattttag cgaagtatct gattccacac
      121 tgccaagaaa agcctctatc gagtaaattg gtgcccgtac cgcaaaccga cacaggtagg
      181 tgaggagaga atcctaagac catcggaaga attgctgtta aggaactagg caaattaacc
      241 ccgtaacttc gggagaaggg gtgcctacga aagtaggccg cagtgaatag gcccaagcaa
      301 ctgtttagca aaaacacagg tctctgctaa agcgaaagct gatgtatagg ggctgacgcc
      361 tgcccggtgc tggaaggtta aggggaatac ttagcgtaag cgaaggt
//
LOCUS       DBGJFF010000332          396 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.348, whole genome shotgun sequence.
ACCESSION   DBGJFF010000332 DBGJFF010000000
VERSION     DBGJFF010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.348"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(<1..286)
                     /locus_tag="ACOIAF_003983"
     CDS             complement(<1..286)
                     /locus_tag="ACOIAF_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670911.1"
                     /translation="MISMIMMLIGVLFIVFGIILTAVEIKGKMPGFYGVGQNAVRKNA
                     IGIIAAGIALIFFRNGLDSIDSILTYGVFLVIELVVIGLYFLIRIKYDKDG"
BASE COUNT          149 a           76 c           23 g          148 t
ORIGIN      
        1 tcccatcttt atcatatttt attcttataa gaaaatataa tcctattact actaactcta
       61 tcactaaaaa tactccatag gtaagaattg aatcaatact atctaaccca tttctaaaaa
      121 aaattaatgc tatacctgct gctataatcc ctatagcatt ttttcttact gcattttgcc
      181 caactccata aaaacctggc atttttcctt ttatttcgac agcagttaaa attatcccaa
      241 acactataaa taatactcca ataagcatca taatcatact aatcatctaa ttacctcctt
      301 cacttaagga aatttcaatt tctaaaatct aaaataaata atatttattt tttataataa
      361 tattgtatat ccaattaact attttggaaa ttagta
//
LOCUS       DBGJFF010000333          386 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.350, whole genome shotgun sequence.
ACCESSION   DBGJFF010000333 DBGJFF010000000
VERSION     DBGJFF010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.350"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            <1..>386
                     /locus_tag="ACOIAF_003984"
     CDS             <1..>386
                     /locus_tag="ACOIAF_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGZ2670912.1"
                     /translation="VWFPKIYYQMEKGLLHIRCEITLGKYQDQLLRLEDKLESGLYCE
                     LTDKTLHDGYIEYTLLYDMIANRITIDEVRAENGCLRLMKNLVWEYDALPHALIAGGT
                     GGGKTYFLLTLIEALLHTNAILYILDP"
BASE COUNT          108 a           79 c           89 g          110 t
ORIGIN      
        1 gtctggtttc caaagattta ttatcaaatg gaaaagggac tgcttcatat ccgctgtgaa
       61 attacgctgg ggaaatatca agaccagctt ttacggttag aggataaatt ggaaagtggc
      121 ttgtattgtg agctgaccga caagaccctg catgacggct atatcgaata taccctgctt
      181 tatgatatga tagcgaaccg cattaccatt gatgaagtac gggcagagaa cggctgtctt
      241 agactgatga aaaatcttgt ctgggaatat gacgcactcc ctcacgcttt gattgctggt
      301 gggactggtg gcggtaaaac atattttctg ctgacactca ttgaagcctt actgcatacc
      361 aacgctatcc tttatatctt agaccc
//
LOCUS       DBGJFF010000334          377 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.351, whole genome shotgun sequence.
ACCESSION   DBGJFF010000334 DBGJFF010000000
VERSION     DBGJFF010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..377
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.351"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            91..>377
                     /locus_tag="ACOIAF_003985"
     CDS             91..>377
                     /locus_tag="ACOIAF_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGZ2670913.1"
                     /translation="MIVVEKAYKFRMYPNKKQQELINKTFGCCRFVYNKYLAKRIDVY
                     KKHKETFTYKQCSSDLTNLKKELKWLKEPDKFSLQNALRDLDNAYKKFFKE"
BASE COUNT          172 a           36 c           53 g          116 t
ORIGIN      
        1 gtgattaata aattgttcac cacagcatta tataatatat taaatatgat aaaatcatta
       61 aaaagtgtat caaaaaacaa ttaaagtgag gtgatagtag tggaaaaagc ttataagttt
      121 agaatgtatc caaataaaaa acagcaagag ttaattaaca aaacttttgg atgttgtaga
      181 tttgtgtata ataagtatct tgctaaaaga atagatgttt ataaaaaaca taaagaaact
      241 tttacttata aacagtgtag ttctgatttg accaatttaa aaaaagaatt aaagtggctt
      301 aaagaacctg acaaattttc actacaaaac gccttaagag atttagataa tgcatataaa
      361 aaattcttta aagaaaa
//
LOCUS       DBGJFF010000335          376 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.352, whole genome shotgun sequence.
ACCESSION   DBGJFF010000335 DBGJFF010000000
VERSION     DBGJFF010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.352"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
BASE COUNT          166 a           31 c           58 g          121 t
ORIGIN      
        1 atctatagta tatttttata aacaagtaat gttattaaaa ttaagtatat agttaaaagt
       61 gtatagcaaa aaggctgtta tattaatgtt agaggttcag ggaaaatata taaagcaaag
      121 atatttacag ataatgtaga ttgcacagct ataagttaga taatagaatt atgtaatcaa
      181 gaatttacaa caaagtataa gataaggata atgctaaatt gccatacagg tgctagatgt
      241 gtaaatggat ttaattaata ctattgataa agtgcaacaa gcacataaac caattgatga
      301 gattattgaa actataagag atatagttga cataattaaa atattgaaac ctgtttataa
      361 ttttaaatca aatcaa
//
LOCUS       DBGJFF010000336          363 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.354, whole genome shotgun sequence.
ACCESSION   DBGJFF010000336 DBGJFF010000000
VERSION     DBGJFF010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.354"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            167..283
                     /gene="rrf"
                     /locus_tag="ACOIAF_003986"
     rRNA            167..283
                     /gene="rrf"
                     /locus_tag="ACOIAF_003986"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            290..361
                     /locus_tag="ACOIAF_003987"
     tRNA            290..361
                     /locus_tag="ACOIAF_003987"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:322..324,aa:Asn,seq:gtt)
BASE COUNT          116 a           61 c           78 g          108 t
ORIGIN      
        1 ttcattaaga tatatgtgca gtttttagag tgttaactct atgtaaagta ctaacttcaa
       61 ttgatgaagt atatatcgta aaaacaatta ctatttagta cttcaaaact attgacattt
      121 aaaaataaag atgttaatat aatacttgtc ttataaatag gcaatatgtg gttataatag
      181 caaagaggat acacctgttc ccattccgaa cacagaagtt aagctcttta gcgctgatgg
      241 tacttggtgg gaaactgcct gggagagtag gacgtagcca cgtgaatatt ccaaggtagc
      301 tcaatggtgg agcaaccggc tgttaaccgg taggctgtgg gttcgagccc cacccttgga
      361 gcc
//
LOCUS       DBGJFF010000337          361 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.355, whole genome shotgun sequence.
ACCESSION   DBGJFF010000337 DBGJFF010000000
VERSION     DBGJFF010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.355"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
BASE COUNT          124 a           44 c           17 g          176 t
ORIGIN      
        1 atttttacat taattataaa tatattaacc tattgttata aattctaaaa aagaaaagtt
       61 atcttaaata aaaaataagt gtatcttaaa ttttaaatga tacacttatt tttatattga
      121 aattatattt tttaatatct attcttaatt aggtattttt aaattatctg tttacattaa
      181 atatctctta attacttatc tttcagctag ctttttctat taaatgtttc tcaattactt
      241 atttttcaat tagccacttc tattaagcat ttcttaatta cttatttttc aattagtcac
      301 ttctattaaa catttcttaa ttatttattt ttcaattagc cacttctatt aaacatttct
      361 t
//
LOCUS       DBGJFF010000338          331 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.356, whole genome shotgun sequence.
ACCESSION   DBGJFF010000338 DBGJFF010000000
VERSION     DBGJFF010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.356"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            complement(<1..329)
                     /locus_tag="ACOIAF_003988"
     rRNA            complement(<1..329)
                     /locus_tag="ACOIAF_003988"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           78 a           91 c           55 g          107 t
ORIGIN      
        1 ttacgatacc tcttcgttat gatatgtccg caaccccaat aaagaaaact ttattggttt
       61 gggctaatcc gcgttcgctc gccgctactt acggaatcga atttctttct cttccttcag
      121 gtacttagat gtttcagttc cctgagttcc cctcattaag ctatgtattc acttaatgat
      181 acttagacat tactctaagt gagtttcctc attcggaaat cttcggatca aagtttacgt
      241 gcaactcccc gaagcttatc gcagcttatc acgtccttca tcggctccta gtgccaaggc
      301 atccgccctg cacccttaat aacttgacca g
//
LOCUS       DBGJFF010000339          287 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.358, whole genome shotgun sequence.
ACCESSION   DBGJFF010000339 DBGJFF010000000
VERSION     DBGJFF010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.358"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            <1..>287
                     /locus_tag="ACOIAF_003989"
     CDS             <1..>287
                     /locus_tag="ACOIAF_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670914.1"
                     /translation="YGTGVYKEPKLVSVKVPVFSMSKLSKVEVSLGPEMKSTGEVLGV
                     GENLEEALYKGFLAAGRHMSDERGVVLATVNNHDKDEFIEIAKDMKELGYT"
BASE COUNT          112 a           30 c           65 g           80 t
ORIGIN      
        1 tatggtacag gagtttacaa agagccaaaa ttagtatcag taaaagtacc agtattctca
       61 atgtctaaat tatctaaagt tgaagtaagt ctaggacctg aaatgaagtc tacaggagaa
      121 gttctaggtg taggagaaaa tttagaagaa gccttatata aagggttctt ggcagcaggt
      181 agacatatgt ctgatgaaag aggagtagta cttgctactg taaataatca tgataaagat
      241 gagtttatag agatagcaaa agatatgaaa gaattaggat atacttt
//
LOCUS       DBGJFF010000340          277 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.359, whole genome shotgun sequence.
ACCESSION   DBGJFF010000340 DBGJFF010000000
VERSION     DBGJFF010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..277
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.359"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            <1..>277
                     /locus_tag="ACOIAF_003990"
     CDS             <1..>277
                     /locus_tag="ACOIAF_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HGZ2670915.1"
                     /translation="ESGLYCELTDKTLHDGYIEYTLLYDMIANRITIDEVKAENGCLR
                     LMKNLVWEYDALPHALIAGGTGGGKTYFLLTLIEALLHTNAVLYILDP"
BASE COUNT           77 a           58 c           63 g           79 t
ORIGIN      
        1 tggaaagtgg cttgtattgt gaactgaccg acaagacctt acatgacggc tatatcgaat
       61 ataccctgct ttatgatatg atagcgaacc gcattaccat tgacgaagtg aaagcagaaa
      121 acggctgtct tagactgatg aaaaatcttg tatgggaata tgacgcactt cctcacgctc
      181 tgattgctgg tggaactggt ggcggtaaaa cgtattttct gctgacgctt attgaagctc
      241 tgctacatac gaatgctgtc ctttacatct tagaccc
//
LOCUS       DBGJFF010000341          275 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.360, whole genome shotgun sequence.
ACCESSION   DBGJFF010000341 DBGJFF010000000
VERSION     DBGJFF010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..275
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.360"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            <1..182
                     /locus_tag="ACOIAF_003991"
     CDS             <1..182
                     /locus_tag="ACOIAF_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HGZ2670916.1"
                     /translation="VEIIYSEKISEVENMLKIENIQYEFTSRDGFIFFGFYDMTDAYR
                     FETLCSDEKIRFKRI"
BASE COUNT          109 a           19 c           42 g          105 t
ORIGIN      
        1 tggtagaaat tatatatagc gaaaaaataa gcgaagtaga aaacatgttg aaaattgaaa
       61 atatacaata tgaatttact tctcgtgatg gctttatatt ttttggtttt tatgatatga
      121 cagacgcata tagatttgag actttatgtt cagatgaaaa aattcgattt aaaagaatat
      181 aatagataaa aaagtacata tttctaaact atttgttttt caaatttagg ttgtgtttta
      241 atgtagtgtt gatattataa acaattttaa taata
//
LOCUS       DBGJFF010000342          269 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.denovo.361, whole genome shotgun sequence.
ACCESSION   DBGJFF010000342 DBGJFF010000000
VERSION     DBGJFF010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..269
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.denovo.361"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            <1..>269
                     /locus_tag="ACOIAF_003992"
     rRNA            <1..>269
                     /locus_tag="ACOIAF_003992"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           80 a           61 c           76 g           52 t
ORIGIN      
        1 agctgatttc acactgtcaa gaaaagtctc tatcgaggtt aaaggtaccc gtaccgcaaa
       61 ccgacacagg tgggtgagga gagtatccta aggccagcga gagaactgtt gttaaggaac
      121 tcggcaaaat gaccccgtaa cttagggata aggggtgcca ccatcaggtg gccgcagaga
      181 ataggcccaa gcgactgttt accaaaaaca taggtttctg ctaagtcgca agacgatgta
      241 taggagctga cgcctgcccg gtgctggaa
//
LOCUS       DBGJFF010000343        24146 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.guided.055, whole genome shotgun sequence.
ACCESSION   DBGJFF010000343 DBGJFF010000000
VERSION     DBGJFF010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24146
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.guided.055"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            <1..1631
                     /locus_tag="ACOIAF_003993"
     CDS             <1..1631
                     /locus_tag="ACOIAF_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VirB4-like conjugal transfer ATPase, CD1110
                     family"
                     /protein_id="HGZ2670917.1"
                     /translation="DRMLADILDLETGVIVNLHIRSIDQSEAIKTIKRKITDLDKMKI
                     EEQKKAVRSGYDMDIIPSDLATFGSEAKNLLQDLQSRNERMFLLTFLVVNMADTKRKL
                     ENDIFAAAGIAQKYNCRLTRLDYQQEAGLLSSVPIGENLIPIQRGLTTSSTAIFIPFI
                     TQELFQTGQALYYGLNALSNNMILCDRKQLKNPNGLILGTPGSGKSFAAKREMTNAFL
                     ITDDDIIICDPEAEYFSLVQRLNGQVIRLSPTGRGIDGKPQYVNPMDINLNYSEDDNL
                     LALKSDFILSLCELVIGGKEGLQPVDKTVIDRAVRNVYRPFLADPDPAKMPILGDLYD
                     ELLRQPEPEAARIAAALELYVSGSLNVFNHRTNVELSNRLVCFDIKQLGKQLKKLGML
                     IVQDQTWNRVTINRAEKKSTRYYMDEFHLLLKEEQTAAYSVEIWKRFRKWGGIPTAIT
                     QNVKDLLASREVENIFENSDFVLMLNQAQGDRTILAKQLNISPQQMKYVIHTEAGEGL
                     IFYGNVVLPFVDRFPKDTELYRVMTTKPEEVSSL"
     gene            1628..2587
                     /locus_tag="ACOIAF_003994"
     CDS             1628..2587
                     /locus_tag="ACOIAF_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HGZ2670918.1"
                     /translation="MKTDVIINRDALAALKELPSESVHCCVTSPPYYALRDYGLDAQI
                     GQEDTPKQYIDRLTAVFGELYRVLRKDGTLWLNIADTYCSTGNKGGYADPKNPKGRTG
                     QRVARNSRVTGCKQKDLIGIPWLLAFSLREQGWYLRSDIIWQKQNPMPESCKDRPTRC
                     YEHIFLLSKEKKYYYDAAAIAEPIAPTTAERYRRARSTNSKYTQEISGQGKVQGLNRP
                     RDGGYYDDALMPTTRNKRDVWLINTVPYKGAHFAAFPPKLAETCILAGCPKGGIVIDP
                     FFGSGTTGFAAKSLDRHYIGIELNAEYCALARVRIGGAGLSSN"
     gene            2614..4554
                     /locus_tag="ACOIAF_003995"
     CDS             2614..4554
                     /locus_tag="ACOIAF_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002590569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HGZ2670919.1"
                     /translation="MTRDGVVEVNETQQTAERISSREADGDFSQPDSAAAERVIEHLD
                     AAHTRKANKKAVKKEQEATALRTSTSRLQFTDEERATPELEPYIKKSEKAADKLDAAK
                     AAIPKQKKLVKERTFEEATGKAKTRLHFEEQEKPIPGGKKGNPLSRPAQEAGIFVHNK
                     IHSVEKDNSGVEGAHKSEELAERGAKYGARKLKQGYRSHKLKPYREAAKAEKAAFKAN
                     VNFQYHKTLHDNPQLTSNPLSRFMQKQQIKCQYAKAAKKGGAKAAAEATRKTAKKTAE
                     ETRKAAAFAARHPAGVLIAVAALLLFIMISVGLSSCGAMFSGLTNGVLGTSYTSEDSD
                     LVAVENNYAALENGLQSEIDAIESTHPGYDEYRYDLDSIGHNPHELASYLTALLQSYT
                     PQSAQAELERIFEKQYTLTLTEEVEIRYRTETSTDPETGETTTEEVPYEYYILNVKLE
                     NKPISSFVSELLTPQQLEMFHVYLETSGNKPLIFGGGSPDGSPSEDLSGVEFVNGTRP
                     GNQELMELAKQQVGNVGGYPYWSWYGFNSRVEWCACFVSWCYNQAGKSEPRFAGCEWQ
                     GVPWFQSRGQWGARGYENIAPGDAIFFDWDLDGVADHVGLVLGRDGSRVYTVEGNSGD
                     ACKIKSYDLNYQCIKGYGLMNW"
     gene            4586..4855
                     /locus_tag="ACOIAF_003996"
     CDS             4586..4855
                     /locus_tag="ACOIAF_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HGZ2670920.1"
                     /translation="MANNKIERINKEIAKTREKITEYQNKLKGLEAQKTEAENLEIVQ
                     LVRAMRLTPQELNAMLSGGGIPGMNAAPATETADYEEQEENADEE"
     gene            4845..5573
                     /locus_tag="ACOIAF_003997"
     CDS             4845..5573
                     /locus_tag="ACOIAF_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HGZ2670921.1"
                     /translation="MKNKLKKTMTALCAALILIGGFSVPAYAQGAATEQPAGDATNDS
                     NVVVEEKEETPPLTPKGNATLVDDYGGNKQLITVTTKSGNYFYILIDRAAEGEQTVHF
                     LNQVDEADLMALTENGEAAKKPEICNCTEKCEAGKVNTSCLVCVTSMTSCTGKEPTPT
                     PTEQPEEPKEKGGNPGVVLALVLFALLGGGAAFYYFKFMKPKQNVKGDTDLEDFEFED
                     YDEDEPEADTGETPEDEETEEETL"
     gene            5570..5758
                     /locus_tag="ACOIAF_003998"
     CDS             5570..5758
                     /locus_tag="ACOIAF_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670922.1"
                     /translation="MTLFTDNPFEKMMIQKPNGRRDNAPPVPYPPACASCPYKGQSPC
                     VGYCLKQVQKKKETEPER"
     gene            5775..7865
                     /locus_tag="ACOIAF_003999"
     CDS             5775..7865
                     /locus_tag="ACOIAF_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HGZ2670923.1"
                     /translation="MILVIAEKPSVAQTIAAVLGVKEKKDGFLTGSGYIVSWCVGHLV
                     GLAEAAAYGEQYKKWNYDSLPILPQEWKYTVAADKEKQFKTLKELMHRADVSEVVNAC
                     DAGREGELIFRFVYEMAGCKKPMRRLWISSMEDSAIKEGFSRLKNGKEYDALFASALC
                     RAKADWLIGINATRLFSVLYNHTLNVGRVQTPTLKMLVDRDAAITTFKKEKYYHVRLA
                     LSGAEAASERISNKAEADTLKTTCEASKAVCTSLLKEKKTAAPPKLFDLTSLQREANR
                     LFGYTAKQTLDLAQALYEKRLLTYPRTDSSYLTDDMGDTAAGIIKLLCGKFPFMAGKD
                     FTPELAKVLNSKKVSDHHAIIPTMELAKTDLAALPESERNILTLAGARLIMATAAPHT
                     FEAVTAVFECAGQSFTARGKTVLSDGWKEIDRKYRAILKSKPETDDADSDTEKTLPPF
                     TEGQNFDNPAAKVTEHDTTPPKPHNEASLLSAMERAGSEDTDPDAERKGLGTPATRAA
                     VIEKLVKGGFVERKGKQLLPTKDGINLVCVLPDTLTSPQLTAEWENNLTQIAKGKADP
                     AAFMKGIEDMARELVKTYPFLSEADKGRFKEEKPELGKCPRCGSPVYEGKKNYYCSNK
                     ECSFTMWKNDRFFEERKVTFTPKIAAALLKSGKVNVKKLYSPKTGKTYDGTIALADTG
                     EKYVNYRIELSKKK"
     gene            7998..12029
                     /locus_tag="ACOIAF_004000"
     CDS             7998..12029
                     /locus_tag="ACOIAF_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008727275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HGZ2670924.1"
                     /translation="MASLRDTVKDYQEELRDGIAWVAFWKTGRSWNAEHFSLEMGDIL
                     YPEDRSRMEEIKQADPAAVVVNGYYSGYLGEDMSLDELTAGVRRHYENGYSNIGEFIE
                     AHDNRLPPELIEEARAAAHAAGLPFSEKAYRDGEEPDPYIFDGSMSMEDYELMHRMIE
                     NERSERMEETILSGYLSNLGKYTEGRPAGEWVSFPTTAEHLKEAFDRIGIDFKNYGEW
                     YFTEFQSSVPGLTTHLNEFSHPDELNYLSELLKMQFDDDREKFVAAMEYGDHTRDLQD
                     IINLAQNLDCYWLYPSIQCEEDYGHYLIEELDELELPEEAKKYFMYEEYGRDAAINDG
                     GSFTEQGYIYNNRNTFTEWYDGCEVPEEYRVTPQPPVQEKEQADLDTSVAIQTTATEQ
                     PPVLPIILSSEKPADKMKEITDRLEQGILGLYESDRYADYLRTMSKFHDYSLNNTILI
                     AMQGGNLVKGYKQWEKEFDRHVKPGEKAIKILAPSPFTVKKQVEKIDPDTQKPVFDKD
                     GKPVTEEREIKIPAFRVVSVFDISQTEGKELPALTYELTGNVEQYKDFFAALEKTFPF
                     AMGFEALSGGVKGRCNYEEKRIIINEGMDELQNIKTAIHEIAHATLHDTALAMPERPD
                     RRTREVQAESVAYAVCQHYGLDTSDYSFGYIAGWSSGKELAELKGSLETIRSTAASLI
                     DTIDGHFAEIQKTQNKEQTTEQAQPAQETAKQPETEIAAPELPEEKAPAKEQDGQPEQ
                     TTQTAAPYYTINEAAAKRAKDMNSFSDYKQGSATAEYRHYVDEAVQIAERQKQRVDPM
                     YHEKIDSLLDTYARKLAANMNKGYEIDARVPSILIAGGSNFPTRKKEKQNAARDSNYR
                     EWQDIQGLLDKIRSTGMGGISADDPQAVQKLEKKLESLEKSQETMKAVNAYYRKHKTL
                     DGCPHLPPEELEKLKADMASSWHLEDKPFATWALSNNSAEIRRVKDRIKSLSQQKEIG
                     FVGWEFDGGKVEANTEANRLQIFFEDKLDETTREALKSNGFRWSPKAGAWQRQLTSNA
                     YYAADYVKAIAPLTGEKPTELQWAHIRAQKVAAQEQPAQEQPEQEAPQDKDTFSIYQI
                     KGEEETRDLRFEPYDRLTATGHRVDPKNYALVYSAELTPGTSLEDIYTRFNIDHPKDF
                     KGHSLSVSDVVVLHQNGQDTAHYVDSFGYKEVLEFFKEQEKALIPDDLETGETIKTPR
                     GTFYVTAMSREQMEAAGYGLHHQSDDGKYLIMGNGTRAFAVPVQPENYLKAAEQTTEQ
                     NYNMIDGQINNTPTTAELEAKVKAGETISLVDLAEAVKADKERGKAAKTEKKPSIRAQ
                     LRADKEKTAQKKAAKSKNHELEV"
     gene            12032..12238
                     /locus_tag="ACOIAF_004001"
     CDS             12032..12238
                     /locus_tag="ACOIAF_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HGZ2670925.1"
                     /translation="MIRLTVEETNLLSIYNEGGKRGLMENINAALPFMDEDMRELAKR
                     TLAKIAPLTENEYAELAIFAADEV"
     gene            12235..12600
                     /locus_tag="ACOIAF_004002"
     CDS             12235..12600
                     /locus_tag="ACOIAF_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HGZ2670926.1"
                     /translation="MTGLKRLTPPQSRKVNSLVKKECCNCDNGHCILLDDGEECVCPQ
                     LISYSLLCKWFQTAVLPLDKLLYAELFKTEDKKRCTECGASFASTSNNVKYCPDCRKR
                     ITRRQAAERMRKRRALVTQ"
     gene            complement(12778..13197)
                     /locus_tag="ACOIAF_004003"
     CDS             complement(12778..13197)
                     /locus_tag="ACOIAF_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HGZ2670927.1"
                     /translation="MNERERIIHLWFDMWLKQQDLGIDSIFTEDVIYTESWSPKYESR
                     KTVKHWFDEWNTRGKVLVWDIKQFFHKENQTIVEWYFKNKMNNGNIEEFDGISLIVWT
                     ENNKIMELKEFGCNLNTYNPYAQNDMPQFRDEKTKWF"
     gene            complement(13190..13576)
                     /locus_tag="ACOIAF_004004"
                     /pseudo
     CDS             complement(13190..13576)
                     /locus_tag="ACOIAF_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728512.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6050 family protein"
     gene            complement(13581..13811)
                     /locus_tag="ACOIAF_004005"
     CDS             complement(13581..13811)
                     /locus_tag="ACOIAF_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGZ2670928.1"
                     /translation="MEQEMPDYETICAAVVGEKWALEKVLDCYGDEINRLAMVKKCQP
                     DGTIKEEIDEDLRQTLIMKLLEAIPQFPMEKE"
     gene            complement(13904..15253)
                     /locus_tag="ACOIAF_004006"
     CDS             complement(13904..15253)
                     /locus_tag="ACOIAF_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005928627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HGZ2670929.1"
                     /translation="MAVTKIKPIKSTLSKALDYIQNPDKTDGKMLVSSFGCSYETADI
                     EFGFTLAQAIEKGNNLAHHLIQSFEPGEVDYEKAHEIGKQLADAVTKGQHEYVLTTHI
                     DKGHIHNHIIFCAVNFVDYHKYNSNKRSYYGIRNMSDKLCRENGLSVVVPGKGSKGKS
                     YAEYQAEKVGASWKGKLKITVDTLIPQVASFEELLQRLQAAGYEIKPGKYISCRAPGQ
                     ERFTRLKTLGADYTEEAIKERIEGKRTKAAKAPKEQRGVSLLIDIENSIKAAQSRGYE
                     QWAKIHNLKLAAKTMNFLTEHKIEQYADLVSRIEEVNAENEKTADALKSVEKRLANMA
                     VLMKHVTTYQKTKPVYEAYRRAKDKDAYRAKQESSLILHEAAAKALKTAGVSKLPNVA
                     ALQVDYEKLREQKEALYADYGKLKKQVKEYDVIKQNIDSILRQPREPEREKGKERGE"
     gene            complement(15214..15543)
                     /gene="mobC"
                     /locus_tag="ACOIAF_004007"
     CDS             complement(15214..15543)
                     /gene="mobC"
                     /locus_tag="ACOIAF_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002610348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HGZ2670930.1"
                     /translation="MNGRKRKIQIKFYVTEEERALIEQKMKLVPTRNMAAYLRKIAID
                     GYIIQTDHSDIKAMTAEIQKIGVNINQIAKRVNATGSVYQEDIEEIKGVLAEIWRLQR
                     LSLLKAR"
     gene            complement(15792..16145)
                     /locus_tag="ACOIAF_004008"
     CDS             complement(15792..16145)
                     /locus_tag="ACOIAF_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGZ2670931.1"
                     /translation="MAKRPVEKYDFKAFGEAIKAARTGRKESRKQVSDEMYISPRYLA
                     NIENKGQHPSLQIFFELMLRYNISVDQFLLDTPAPKDTKRRQLDALLDNMSDNGIRIV
                     TATAKEISEVEKEDK"
     gene            16307..16495
                     /locus_tag="ACOIAF_004009"
     CDS             16307..16495
                     /locus_tag="ACOIAF_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGZ2670932.1"
                     /translation="MEYMSCPEAAKKWGISERRVQILCREKRIQGVSKLGYMWLIPKD
                     AEKPIDGRTKQGKELYHE"
     gene            16488..17180
                     /locus_tag="ACOIAF_004010"
     CDS             16488..17180
                     /locus_tag="ACOIAF_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGZ2670933.1"
                     /translation="MNKVLIIDDDKELCALIKRSVLSEDIEADFCNTGKEGLQKLKER
                     EYQLVILDIMMPGMDGFETLEEIRKENSLPILMFTSKNDSASKVRGLRAGADDYLTKP
                     FDMDELIARIISLIRRYTRFNQQDGISQQLEFDGLKIDIENRSVTTENGTFELPPKEF
                     DLLLYCAKNQGKILTKQQIYEEVWREEYFYDDSNIMAIISRLRKKLEVNPGSPKYIQT
                     IKGIGYRFNKEV"
     gene            17187..18104
                     /locus_tag="ACOIAF_004011"
     CDS             17187..18104
                     /locus_tag="ACOIAF_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HGZ2670934.1"
                     /translation="MEAVIFLSIIIALFSILVSCFAIRRVKKQIAEITDALIDVKNGN
                     GNRRILSATNELVAPLAYEINEIVVSYESRLSTVRQTEETNRQLMTSLSHDVRTPLTT
                     LIGYLDAAHKGIVTGKDRDDYIETARRKAHDLKEYIDVLFDWFKLNSNEFAMDINIVE
                     AAELTRNILIDWIPIFEDKQIDYNIDIPEQPFRVKLDTDGYMRILNNLIQNVISHSHA
                     DKIEIILSKQEKNMQIRLADNGIGIEKEDLKHIFERLYKCDKGRSEKGSGLGLSIAHQ
                     LVEKMNGTITANSIQGTGTEFTLLFPLAN"
     gene            18241..19167
                     /locus_tag="ACOIAF_004012"
     CDS             18241..19167
                     /locus_tag="ACOIAF_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2670935.1"
                     /translation="MDKNYIIETKNLTKQYGSQKSVADLNIHVQKGRIYGLLGRNGAG
                     KTTTMKMLLGLTKPTSGEVKIWGKSLQGNEKKLLPRIGSLIESPGFYPNLTATENLQI
                     FATLRGIPNRHSIKNTLDLVGLPYKDKKLFSQYSLGMKQRLAIALAVMHDPELLILDE
                     PINGLDPIGIAEVRSFIRELCDTKGKTILISSHILSEISLLADDIGIIDHGTLLEEES
                     LAELEQKSSKHIRFTLSDTAQAARILERTFHESHFSIQDDHNLRLHNLDLSVGKIVTA
                     FVENGLEVSEAHTCEESLEDYFKRVTGGEGIA"
     gene            19160..19969
                     /locus_tag="ACOIAF_004013"
     CDS             19160..19969
                     /locus_tag="ACOIAF_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2670936.1"
                     /translation="MLKLIKCEFWKLKRKRLLYALMSLSLFFPLILAYMAKAGTGADT
                     TEHYLQTRFDYVYTMMLGYGLVFLLPCLIGIIAAILFFIERDCDTSKNLRTIPVTNTQ
                     LIMAKISMLFIFSVAFCLISTLSVALFCKLFHVGMVYGMTYKIFMSLIFGVLIVAASL
                     PIVFLIIYFNKSFLLSILLAFFYSIFNWGILGTIGTSISAAKIAFLNYFPVICVMNWT
                     SGLMMDNLQKDNLLPEAYAIVPTTCHTIFIMAITVILSLWLIIRFYKKWTR"
     gene            19975..20748
                     /locus_tag="ACOIAF_004014"
     CDS             19975..20748
                     /locus_tag="ACOIAF_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2670937.1"
                     /translation="MRNIIKTEFLKLKRYSVIKAGIIMTTLSPLLSLFYSTANGGQTW
                     TFDYFMQQVMISNCTLFFPIIIALIAGYIITREYTDDTMKNILTIPIPYKQLLSGKLL
                     ILLLLTISFSLIGCVIALVINIIVGFPGVHFGNLLNMFIRVTGANIGIYISVLPIILI
                     FCCSANNFLGGVALAFVYGYFGTFEGTLLNYYPIKASMILVDPTCGTEYGYTYHIFPA
                     FITIVLTFLISMIILANKKKEPGTLTVGKKKKAVRKKGW"
     gene            21221..21661
                     /locus_tag="ACOIAF_004015"
     CDS             21221..21661
                     /locus_tag="ACOIAF_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009255547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HGZ2670938.1"
                     /translation="MEPNRREFQKQCAFQSFCKRVLHNEACNAHDEIRRRRKREVSFC
                     DLALHEERQLYTTDKYFQAETAEPTYQMAGKEITPKLLLEAIRTLPEEKRTAILLYYF
                     EGMTDVEIGKMFNTSRSTIQYRRTSSFELLKKYLEEHADEWNEW"
     gene            21642..21899
                     /locus_tag="ACOIAF_004016"
     CDS             21642..21899
                     /locus_tag="ACOIAF_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGZ2670939.1"
                     /translation="MNGTNGNQNDYPENALVPYPVILAASKGDPDAMKMVLQHFSGYI
                     ASLSMRKLYDERGNVYYGVDEDIRERLQARLMRAILTFRAE"
     gene            22321..22524
                     /locus_tag="ACOIAF_004017"
     CDS             22321..22524
                     /locus_tag="ACOIAF_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HGZ2670940.1"
                     /translation="MNNNDVPIWEKYTLTIEEAAKYFRIGESKLRKLAEENPVPDWVM
                     MNGNRIQIKRKQFEKMIDTVDAI"
     gene            22604..23797
                     /locus_tag="ACOIAF_004018"
     CDS             22604..23797
                     /locus_tag="ACOIAF_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005343085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGZ2670941.1"
                     /translation="MSEKRRDNKNRILRTGESQRKDGRYAYKYIDTFGKPQFVYSWKL
                     VPTDKTPAGKRDDIALREKEKEIQKDLDDGIDHIGKKMTVCQLYAKQIRHRANVRHGT
                     KQGRKQLMRILQEDKLGACRIENVKLSDAKEWALRMKEKGYGFKTINNHKRSLKAAFY
                     TAIQDDCIRKNPFDFQLNTVLEDDTEPKEPLSPTQEAAFLSFVQHDKVYQKYYDEIII
                     LLGTGLRISELCGLTEADIDLDKQLINVDHQLLKIADVGYYVETPKTKSGNRIIPMSE
                     KVLEAFQRVLNKRKYTQPVILEGYTKFLFLNRNGLPKVAVNYESMFRGLVRKYNKTQK
                     VALPKVMTPHTLRHTFCTTLANAGMNPKALQYIMGHSNINMTLNYYAHTTSETSITEM
                     KRLIA"
BASE COUNT         7110 a         5483 c         5647 g         5906 t
ORIGIN      
        1 acgaccgtat gcttgccgac atactggact tggaaacagg cgtaatcgtc aacttacata
       61 tccgcagtat cgaccagtcg gaagcaatca agaccattaa gcgcaaaatc acagaccttg
      121 acaagatgaa gattgaggaa cagaaaaaag cggttagaag cggctatgac atggatataa
      181 tcccgtccga ccttgccacc tttggcagcg aagcaaagaa cctgttgcag gacttacaga
      241 gccgcaacga gcgaatgttt ctccttacgt tccttgtggt aaacatggca gacacgaagc
      301 ggaaactgga aaatgacatt ttcgcggcgg cgggtatcgc acagaaatac aactgccgcc
      361 tgacacgcct tgactatcag caggaagcgg ggcttttatc cagtgtgccg attggggaaa
      421 accttatccc tattcagaga ggtttgacaa cgagcagcac cgctattttc attcccttta
      481 tcacgcagga gctttttcag acggggcaag ccctttacta tggcttgaac gccctttcta
      541 acaacatgat actctgcgac agaaagcagc tcaaaaaccc aaacgggctt atcttgggaa
      601 cgccgggaag cggaaaatcc tttgcggcaa aaagggaaat gacaaacgct tttctcatta
      661 ccgacgacga cattatcatc tgcgacccgg aagccgagta tttttccctt gtgcagcgtc
      721 ttaatgggca agtgatacgt ctttccccta cgggcagggg cattgacggc aagccccagt
      781 atgtgaaccc tatggatata aaccttaatt acagcgagga cgacaacctg ttagccttaa
      841 agtccgattt tatcctttcc ctctgcgagc ttgttattgg cggcaaggag ggcttgcagc
      901 ctgtcgataa gaccgtcatt gacagggctg taagaaatgt gtaccgcccg ttccttgcag
      961 accccgaccc ggcaaaaatg cctatcttgg gcgaccttta cgacgagctg ttaaggcagc
     1021 cggagccgga agctgcccgt atcgcggcgg cattggagct gtatgtttcc ggcagcctta
     1081 acgtattcaa ccacagaacc aacgtggaac tttctaaccg ccttgtctgc tttgatataa
     1141 agcagcttgg gaagcagctt aaaaagttag gtatgctcat tgtgcaagac cagacatgga
     1201 acagggtaac gataaaccgg gcagaaaaga aatccacccg ctactatatg gacgagtttc
     1261 acttgctctt aaaagaggaa cagacggcag cttacagcgt ggagatttgg aagcgtttcc
     1321 gtaaatgggg cggcataccc acagccatta cgcagaacgt caaagacctt ttggcaagcc
     1381 gcgaggtgga aaatatcttt gagaacagcg attttgtcct tatgctcaat caagcgcagg
     1441 gcgaccgtac tatccttgca aagcagctta atatttcccc gcagcagatg aagtatgtta
     1501 tccacaccga agcgggcgag gggcttatct tttatggaaa tgtggtgctg ccttttgtag
     1561 accgcttccc gaaagatacc gagctttacc gggtaatgac gacgaagccg gaggaagtga
     1621 gcagcctatg aaaacagatg tgattatcaa cagggacgca cttgccgcct taaaagaact
     1681 gccctctgaa agcgtccatt gctgcgttac aagcccaccg tactatgcgc tacgggacta
     1741 tgggcttgac gcacaaatcg gacaggagga cacgccgaaa cagtatattg acaggctgac
     1801 cgctgttttt ggcgagcttt accgggtgct tagaaaggac ggtacgttat ggctgaatat
     1861 cgcagacacc tattgcagca caggaaataa aggcggctat gctgacccga aaaacccaaa
     1921 agggcgcacc ggacagcggg ttgcaaggaa cagccgcgtt accggctgca agcaaaagga
     1981 cttaatcggt attccctggc ttttagcgtt ttccttgcgg gaacaggggt ggtatctgcg
     2041 gagcgatatt atatggcaga aacaaaatcc tatgccggaa agctgcaaag accgcccgac
     2101 ccgctgctat gaacatatct tcctgctttc aaaggaaaag aaatattact atgacgctgc
     2161 cgctattgca gaacccatag cccccaccac cgcagaacgg tatcgcagag cgcgcagcac
     2221 taacagtaaa tacacacagg aaatctccgg acagggaaag gtacagggac taaaccgacc
     2281 gcgggacggc ggctactatg acgacgcgct catgccgact acgcggaaca agcgggacgt
     2341 gtggctgatt aacaccgtcc catataaagg ggcgcacttt gcagcttttc ccccgaaatt
     2401 agcggaaacc tgtatcttgg caggctgtcc caaaggtggg attgtcatag acccgttttt
     2461 cggaagcgga accacaggat ttgcagcaaa aagccttgac cgccattata tcgggattga
     2521 attaaatgca gagtattgcg cccttgcgag ggtgcggatt ggaggtgcag gactatcaag
     2581 caattaaaac cacgcgataa aatcacacag aaaatgaccc gcgacggcgt ggtggaagtc
     2641 aacgaaacac agcagaccgc cgagcgtatc agcagccggg aagcagacgg cgacttttca
     2701 cagccggaca gtgccgcagc ggaacgcgtc atagaacatc ttgacgcagc acatacccga
     2761 aaggcaaaca aaaaggcagt caaaaaagag caggaagcta ctgctttgcg tacttccact
     2821 tcccgcttgc agtttaccga cgaggaacgg gcaacgccgg aactggaacc ctacatcaaa
     2881 aaatcagaaa aagcagccga taaactggac gcggcaaagg cagctatccc gaagcaaaag
     2941 aaactggtta aggagcgtac ctttgaggaa gccacaggaa aggcaaaaac ccgacttcac
     3001 tttgaagaac aggaaaaacc gatacccggc ggcaaaaagg gaaacccgct gtcccgcccc
     3061 gcacaggaag caggaatttt cgtccacaac aagatacatt ctgttgaaaa ggacaattcc
     3121 ggcgtggagg gggcgcataa atcggaggaa cttgccgagc gcggcgcgaa atacggggcg
     3181 cggaagttaa aacagggcta ccgcagccat aagctgaaac cttaccggga agcagcaaag
     3241 gcagaaaagg cagcatttaa ggcaaacgtc aacttccagt atcacaagac cctgcacgac
     3301 aacccgcagc ttacttctaa ccctctttcc cgtttcatgc agaaacagca gataaaatgc
     3361 cagtatgcaa aggcggcaaa gaaaggaggg gcaaaagccg cagcggaagc cacaagaaag
     3421 acagcgaaga aaaccgcaga agaaacgagg aaagccgccg catttgcggc aagacacccg
     3481 gcgggtgttt tgattgcagt tgccgcgctg cttctcttta ttatgatttc cgtagggctg
     3541 tcctcttgcg gggctatgtt ttcgggcttg actaacgggg tgcttggcac ttcctacacg
     3601 tccgaggaca gcgaccttgt ggcagttgaa aacaattatg ccgcattgga aaacgggctg
     3661 caaagtgaga ttgacgctat cgaaagcacc caccccggct atgatgaata ccgctatgac
     3721 cttgacagta tcgggcataa cccacatgaa cttgcgtctt acttgaccgc ccttttgcag
     3781 agctacaccc cgcagagcgc acaggcagag cttgaacgta tctttgaaaa acagtacacc
     3841 ttgacgctga ccgaggaagt ggaaatacgc taccgcactg aaacaagcac cgacccggaa
     3901 accggggaaa ccaccaccga ggaagtccct tatgagtatt acatcttgaa tgtcaaactg
     3961 gaaaacaagc cgatttcctc ttttgtgtcg gagcttttaa cgccacagca gcttgaaatg
     4021 ttccatgtgt acttggaaac aagcggcaac aaaccgctga tttttggcgg cggttctccc
     4081 gacggaagcc cgtccgagga tttaagcggc gtggagtttg taaacggtac gcgccccgga
     4141 aatcaagagc ttatggagct tgcaaaacag caagtcggaa atgtgggcgg ctacccttac
     4201 tggtcttggt acggctttaa cagccgcgtg gaatggtgcg cctgttttgt atcatggtgc
     4261 tacaatcaag ccggaaaaag cgaaccccgc tttgcgggct gcgaatggca gggtgtccct
     4321 tggtttcagt caaggggaca atggggcgca cgcggctatg agaatatcgc accgggcgac
     4381 gcaatctttt tcgattggga tttagacggc gttgccgacc atgtggggct tgtgcttggc
     4441 agggacggca gccgcgtcta taccgttgag ggcaattccg gcgacgcctg caagataaaa
     4501 agctatgacc ttaactatca atgtatcaaa ggctacgggc tgatgaactg gtaaagcagc
     4561 cccaaaaaga aaggagaaat gacctatggc aaacaataaa atcgaacgta tcaataagga
     4621 aatcgcaaag acccgcgaga aaatcacaga gtatcagaac aagttaaaag gactggaagc
     4681 gcagaaaacc gaagcggaaa accttgaaat cgtacagctt gtacgagcta tgcgactgac
     4741 cccgcaggaa cttaacgcta tgctgtccgg cggcggtatt cccggcatga acgccgcacc
     4801 cgctaccgaa acggcagact acgaagaaca ggaggaaaat gcagatgaag aataaactga
     4861 aaaagaccat gaccgccctt tgtgccgccc ttatccttat aggcggtttt tctgtccctg
     4921 cctatgcaca gggcgcagcc acagagcagc ccgccgggga cgcgaccaac gacagcaacg
     4981 tagttgtgga ggaaaaagaa gaaacgccgc cccttacccc aaagggaaac gcaacactgg
     5041 ttgacgatta cggcggcaat aagcagctta tcactgttac caccaaaagc ggcaactatt
     5101 tctatatcct cattgaccgc gccgccgagg gcgaacagac cgtacatttc ctaaatcaag
     5161 tggacgaagc cgaccttatg gcactgaccg aaaacggcga agctgccaaa aagccggaaa
     5221 tctgcaactg tacggagaaa tgcgaagccg gaaaggtaaa cacgtcctgc ctggtatgtg
     5281 ttaccagtat gacaagctgc acaggcaaag aacctacccc aacaccaacc gagcagccgg
     5341 aagaaccgaa agaaaaaggc ggcaatcccg gtgtggtgct tgccttagtc ctctttgccc
     5401 tgcttggtgg cggcgcagcg ttctactact ttaagtttat gaagccgaag cagaatgtaa
     5461 agggcgatac cgaccttgaa gattttgaat ttgaggacta cgacgaggac gagccggaag
     5521 cagataccgg ggaaactccc gaagatgaag aaacggagga agaaacccta tgaccctgtt
     5581 taccgacaat ccctttgaaa agatgatgat acagaaaccg aacgggcggc gtgataatgc
     5641 gccgcctgtt ccttatcccc cggcttgcgc ttcctgccct tacaagggac agtccccttg
     5701 tgtggggtac tgtctgaaac aggtacagaa gaaaaaagaa accgagccgg aacgctgaaa
     5761 ggaggaactt atatttgatt ttagttatcg cagaaaaacc cagtgtggcg cagacaatcg
     5821 ccgccgtact tggggtaaag gaaaagaaag acggatttct cacaggaagc ggctatatcg
     5881 tttcttggtg cgtgggacat ttggtagggc ttgcggaagc tgccgcctac ggggaacagt
     5941 ataaaaagtg gaactatgac agcttaccga ttttgccgca ggaatggaaa tacaccgttg
     6001 ctgccgacaa ggaaaagcaa tttaaaaccc taaaagagct tatgcaccgg gcagatgttt
     6061 ccgaagtcgt caatgcctgc gacgcagggc gcgagggaga actcattttc cgctttgtct
     6121 atgaaatggc gggctgcaag aaacctatgc gccgcctttg gatttcttct atggaggaca
     6181 gcgcaatcaa agagggcttt tcccgtctga agaacggcaa ggaatacgac gcgctctttg
     6241 cttccgcact atgcagggca aaggctgact ggctaatcgg tatcaatgcc acccgccttt
     6301 tctctgtcct ttacaatcat accttgaacg tggggcgtgt acagaccccg accttaaaaa
     6361 tgcttgttga ccgggacgca gcgattacca ctttcaagaa agaaaaatac taccatgtgc
     6421 gccttgcctt atccggcgcg gaagctgcaa gtgaaagaat ttcaaataag gcagaagccg
     6481 acacattaaa aacgacttgc gaagcgtcaa aagcagtctg tacttccctt ttgaaagaga
     6541 aaaagaccgc agccccgccg aagctctttg accttacttc tttacagagg gaagcaaacc
     6601 gcctgttcgg atatacggca aagcagaccc ttgaccttgc acaagccctg tatgaaaaaa
     6661 gacttcttac ttatccgaga acagacagca gctatctgac tgacgacatg ggcgacacag
     6721 cagcgggcat tatcaagctg ctttgcggga aatttccttt tatggcggga aaagacttca
     6781 cgccggagct tgcaaaggtg ctgaacagta aaaaggtatc agaccaccat gccattatcc
     6841 cgactatgga gcttgcaaag accgaccttg ccgcgctgcc ggaaagcgaa aggaatatcc
     6901 ttacccttgc cggggcgcgt ctgattatgg cgactgccgc accgcatacc tttgaagcgg
     6961 ttacggcagt ttttgagtgt gccggacagt cctttaccgc aaggggaaaa acggtactgt
     7021 ccgacgggtg gaaagagatt gaccgaaaat acagggcaat cttaaagagt aagcctgaaa
     7081 cggacgacgc agacagcgac acagaaaaga ccctgccgcc gtttaccgag ggacagaact
     7141 ttgacaatcc agcggcaaag gtaacggagc atgacacaac accgccaaag ccccacaatg
     7201 aagcgtcgct cctttccgct atggagcgcg ccggaagcga ggacaccgac ccggacgcag
     7261 aacgcaaagg gcttggaact cccgccaccc gcgccgccgt cattgaaaaa ctggtaaagg
     7321 gcggctttgt ggagcgaaaa ggcaagcagc tacttcccac aaaagacggt atcaaccttg
     7381 tgtgcgtcct gccggacacc ttgacaagcc cgcagcttac cgcagaatgg gaaaacaatc
     7441 tgacgcagat tgccaaaggc aaggcagacc ccgccgcatt tatgaagggt atcgaggata
     7501 tggcgcgaga actggtaaag acctatccgt ttctttccga agctgacaag gggcgtttca
     7561 aagaggaaaa gccggagctt gggaaatgtc cccgctgtgg ttctcctgtc tatgagggaa
     7621 agaaaaacta ctattgcagc aacaaagaat gtagctttac catgtggaaa aatgaccgtt
     7681 tctttgaaga acgaaaagta acctttaccc cgaagattgc cgctgcactc ttaaaatccg
     7741 gcaaggtaaa tgtgaagaag ctctattccc caaagacagg caaaacttac gacggaacta
     7801 tcgcattggc tgatacaggc gagaaatatg tcaactaccg cattgaactg tcgaagaaga
     7861 aataactgaa tagcaagcat aggaaaagag tacccttttc cgtaaaatcc ctgctttctc
     7921 taccgagaac ggcggggatt tttgcttgtt gggaagtttc ccaaaccctc taagacagtg
     7981 aagaaaggag gttacacatg gcaagtttac gggacaccgt aaaggactat caagaagaat
     8041 taagggacgg tatcgcttgg gtggcgtttt ggaaaacggg acgttcttgg aacgccgagc
     8101 atttttccct tgaaatgggc gatattctct acccggagga cagaagccgc atggaagaaa
     8161 tcaagcaggc tgaccctgcc gccgttgtgg taaacggcta ttattccggc tatcttggcg
     8221 aggatatgag ccttgacgag ctgaccgccg gggtacgccg ccactacgaa aacggatata
     8281 gcaatatcgg ggaatttatc gaagcccacg acaacaggct gccgccggag cttatcgagg
     8341 aagcaagagc cgccgcccac gctgcggggc tgcctttctc tgaaaaagcc taccgggacg
     8401 gcgaagaacc cgacccctat atttttgacg ggagcatgag catggaggac tacgaactta
     8461 tgcaccgtat gattgaaaac gaaaggagtg aacgcatgga agaaacgatt ttaagcgggt
     8521 atctttctaa tcttggaaag tacaccgagg gcagacctgc gggcgaatgg gtatcattcc
     8581 ccacgactgc cgagcatttg aaagaagcct ttgaccgtat cgggatagac ttcaagaact
     8641 atggagaatg gtattttaca gaatttcaat cttccgttcc gggtttaaca acacacctta
     8701 acgaattttc ccaccccgac gaactgaact atttgagcga acttttgaaa atgcagtttg
     8761 acgacgaccg ggaaaaattt gttgcagcta tggaatacgg cgaccataca agggacttgc
     8821 aggacatcat caaccttgca caaaaccttg attgttactg gctttatccg tccatacagt
     8881 gtgaggaaga ttacgggcat tatctgattg aagaactgga cgagttggag ttgcccgaag
     8941 aagcaaaaaa atatttcatg tatgaggaat acgggcggga cgctgctatc aatgacgggg
     9001 gcagctttac cgaacagggc tatatctaca acaaccgcaa cacctttaca gaatggtacg
     9061 acgggtgcga agtgccggag gaataccggg taacgccgca gccgccagta caggaaaagg
     9121 aacaggcaga ccttgacact tccgtagcca tacaaaccac cgccacagag cagcccccgg
     9181 ttctcccgat tatcctatct tctgaaaagc ccgctgacaa aatgaaagag attaccgacc
     9241 gactggaaca aggaatttta ggactgtatg aaagcgaccg ttacgccgat tatctgcgta
     9301 ccatgtcaaa attccatgat tacagcctta acaatacaat ccttatcgcc atgcagggcg
     9361 gcaatcttgt gaaaggctat aaacaatggg aaaaggaatt tgaccgccat gtaaagcccg
     9421 gagaaaaagc tatcaagata cttgcgccgt ccccgtttac cgttaagaaa caggtggaaa
     9481 aaatcgaccc ggacacccaa aagcctgttt tcgataagga cgggaaacct gttaccgagg
     9541 aaagggaaat caagataccc gcttttcgtg tggtatctgt ctttgatatt tcacagaccg
     9601 agggaaagga gcttcctgcc cttacctatg agctgacagg caacgtggaa caatacaagg
     9661 attttttcgc tgcccttgaa aagactttcc cctttgctat gggatttgaa gcactaagcg
     9721 gcggcgtaaa aggacgctgc aattatgagg aaaagcgtat catcatcaac gagggcatgg
     9781 acgaattgca gaatatcaag actgcaatcc atgaaatcgc ccacgccacg ctgcatgata
     9841 ccgcccttgc tatgccggaa cgccccgacc gccgcacccg cgaggtacag gcggaaagcg
     9901 tcgcctatgc tgtctgccaa cattacgggc ttgatacgtc ggattattct tttggatata
     9961 tcgccgggtg gagcagcgga aaagaacttg cagaactgaa aggctctctt gaaacaatcc
    10021 gcagtaccgc cgcaagtctg attgatacca tagacggaca ttttgcagaa atccaaaaga
    10081 cacagaataa ggaacagacc accgaacagg cgcagcccgc acaggaaacc gccaaacagc
    10141 ccgaaacaga aatagccgcg ccggagcttc ccgaagaaaa agcccctgca aaggagcaag
    10201 acggacagcc ggagcagacc acgcagaccg ccgcccctta ttacactatc aatgaagctg
    10261 ccgccaaacg tgcaaaggat atgaacagtt tttccgatta taagcaaggc agcgcgacgg
    10321 ctgaatacag gcattatgtt gatgaagccg tacaaatcgc agaaaggcag aaacaacggg
    10381 ttgacccgat gtaccatgag aaaatcgaca gcctgcttga cacctacgcc cggaaactgg
    10441 cggcgaacat gaacaaaggc tatgagattg acgcgcgtgt tccctctatc ctgattgcgg
    10501 gcggctctaa cttccccaca aggaagaaag aaaagcagaa cgcagcccgc gacagcaatt
    10561 accgggaatg gcaggacata caaggacttc ttgataaaat ccgcagcacc ggcatgggcg
    10621 gtatcagcgc agacgaccca caggcagtac agaagttaga aaagaaactg gaaagccttg
    10681 aaaaatcaca ggaaaccatg aaagccgtaa acgcctatta ccgtaagcat aagacccttg
    10741 acggctgccc gcatttaccg ccggaggaac tggaaaaact gaaagcagac atggcgagca
    10801 gttggcattt ggaggacaag ccctttgcga cttgggcgtt atccaacaac agcgcagaaa
    10861 tccgacgcgt gaaagaccgt atcaaatccc tttcacagca aaaagaaatc ggttttgtgg
    10921 gttgggaatt tgacggcggt aaggtggaag caaacaccga agcgaaccga ctgcaaatct
    10981 tctttgaaga taagctggac gaaaccacgc gggaagcctt aaaaagtaat ggcttccgtt
    11041 ggtcgccaaa agcaggggca tggcagcggc agcttaccag taatgcctac tatgcggctg
    11101 attatgtcaa ggcgattgca cctcttaccg gggaaaagcc taccgagtta cagtgggcgc
    11161 atatccgggc gcaaaaggta gctgcacaag aacaacccgc acaggagcag ccggaacagg
    11221 aagccccaca ggacaaagac accttttcca tttatcagat aaaaggcgag gaagaaacaa
    11281 gggacttgcg ctttgaacct tacgaccgtc tgactgccac aggacaccgg gtagacccga
    11341 aaaactatgc gcttgtctat tccgcagagc ttacgccggg gacttccctt gaagatattt
    11401 acacccgctt caatatcgac cacccgaaag attttaaggg acacagcctt tccgtttccg
    11461 acgtggtggt gcttcatcag aacgggcagg acaccgccca ctatgtagac agcttcggtt
    11521 ataaggaagt gctggagttt ttcaaggagc aggaaaaagc cctcattccc gacgacttgg
    11581 aaacgggcga aacaatcaaa acaccgagag ggactttcta tgtaaccgcc atgagccgcg
    11641 agcaaatgga agctgccgga tatggattgc accaccagtc cgacgacgga aaatatctga
    11701 ttatggggaa cggcactagg gcttttgctg ttcccgttca gccggagaac tatctgaaag
    11761 ctgccgagca gaccacagag caaaactaca acatgattga cgggcagata aacaacaccc
    11821 cgactaccgc agaactggaa gcaaaggtaa aggcaggaga aaccatttcc cttgttgacc
    11881 ttgcggaagc tgtcaaggct gacaaggaac gcggcaaggc agcaaaaacg gaaaagaagc
    11941 cctctatccg ggcgcagctt agagcagaca aagaaaaaac cgcacagaaa aaagcggcaa
    12001 aatcaaaaaa tcacgaattg gaggtatgag catgattaga ctgaccgttg aagaaacaaa
    12061 cctgttgagc atttacaatg agggcggtaa gcgcgggctt atggagaaca tcaacgctgc
    12121 gctgccgttt atggacgagg atatgcggga actggcgaag cgcacccttg cgaaaattgc
    12181 acctctgaca gaaaatgagt atgcggagct tgccattttc gccgctgatg aggtatgacc
    12241 gggctaaagc ggcttacgcc gccccaaagc cggaaagtca acagccttgt aaaaaaagag
    12301 tgctgcaact gcgataacgg acactgtatt ttactggacg acggggagga atgtgtctgc
    12361 ccgcagctta tttcctattc gcttctttgc aaatggtttc agaccgccgt acttcccctt
    12421 gataagctgc tatatgcaga gcttttcaag accgaggata aaaagcgttg taccgagtgc
    12481 ggagcttctt ttgcgtccac ctctaacaac gtcaaatact gcccggattg ccggaagcgt
    12541 attacccgca gacaggcagc ggagcgcatg aggaaacgac gcgcccttgt tacgcagtag
    12601 gggcaaaaaa agccttgatt tacagggctt tccgggtgtg aaaatgacgc ggcaagggtt
    12661 ttataccttt tccctcaaaa atggcgttct actgcgtaac attttatcgc tgtacccata
    12721 agaaagccgg ggcgcggata gtctttaccg actgtctgcg tcccggcttt taaaacttca
    12781 aaaccacttt gttttttcat ctctgaattg tggcatatca ttttgggcgt atggattata
    12841 ggtattgaga ttacaaccaa attctttcaa ctccattatt ttgttatttt ccgtccatac
    12901 tattaaagaa attccgtcaa attcttcaat attaccatta ttcattttgt ttttgaaata
    12961 ccattctaca atagtttgat tttccttatg aaaaaattgt ttaatatccc acactaagac
    13021 ttttccacga gtattccatt catcaaacca atgttttact gtttttcgac tttcatactt
    13081 aggactccaa ctttcagtgt aaatcacatc ttctgtaaaa atactatcaa tccctaaatc
    13141 ttgttgtttt agccacatat caaaccataa atgaataatt ctttctcttt cattcataac
    13201 ttttaaatcc tttctctata ttgatttgag gggttgcact cttatctgcg gtaagtaaac
    13261 ggtatatcgt aagggggata taccacgtag caacgacaag ccgccatatt aaaatgcccc
    13321 cgccgataat gccgtccaaa atgaagttaa tggcaacgat acccaccgtt ccagctatgt
    13381 catatccatg cggtacaagc catacgaaca tacggcgaat gccaaagggg ataccgcagc
    13441 acagccatac ataaaagtaa ttggtctacc cgtcctctgt aaagatgttc ttgaacatga
    13501 agaataaaaa caatgcaata acggcgggca aaacgtttct cttgaaaaag tctttccaaa
    13561 tttctccgcg tgtcatggta tcactccttt tccattggaa attgtgggat agcttctaac
    13621 agtttcatta tgagtgtttg ccgtaaatct tcgtcgattt cctctttgat agtcccgtcc
    13681 ggctgacact tttttaccat tgcaaggcgg ttgatttcgt cgccgtaaca atcaagcacc
    13741 ttttccaacg cccatttttc gcccacaacg gcagcgcata tcgtttcata gtccggcatt
    13801 tcctgttcca ttttctgaca cttcctttgc tgaaaatgta tcactttagt atgtggctac
    13861 tgtctttgat aggaactatt atatcacaca agtatgaaca aagctactcc ccgcgttctt
    13921 tccctttttc ccgttccggc tctcttggct gccgtaaaat gctgtctatg ttctgtttga
    13981 taacgtcgta ctctttgacc tgttttttca gtttgccata gtccgcgtaa agggcttctt
    14041 tctgttcccg gagcttttca tagtctactt gtagcgcggc gacattcggg agcttggaaa
    14101 cgcccgccgt ttttagtgcc tttgccgctg cttcatggag tatcagactg ctttcctgct
    14161 ttgcccggta tgcgtcctta tcctttgccc tgcggtatgc ttcatagaca ggttttgtct
    14221 tttggtaggt ggtaacgtgc ttcatcagca ccgccatgtt tgcaagacgt ttctccacgc
    14281 tctttaatgc gtctgctgtc ttttcatttt ccgcatttac ttcctcaatc cggctgacta
    14341 aatctgcgta ctgctcaatc ttatgttctg tcaagaaatt catagtcttt gcagccagtt
    14401 tcagattatg gattttcgcc cactgttcat agccccggct ctgcgctgcc ttgatactgt
    14461 tttcaatgtc aataagcagc gatacgccgc gctgctcttt gggagctttc gccgcctttg
    14521 tgcgcttgcc ctctatccgt tctttgattg cttcctctgt ataatctgca ccgagggttt
    14581 ttaagcgggt aaaccgttcc tgtcccggag cgcggcagga gatatatttt cccggcttta
    14641 tctcatagcc cgctgcctgt aaccgctgca acaattcttc aaaacttgcc acttggggga
    14701 taagcgtatc aacggtaatt ttcagcttgc ctttccaact tgcaccgacc ttttccgctt
    14761 gatattctgc atagcttttt cccttgctgc ccttacccgg aacgacaacg gacagcccgt
    14821 tttcccggca cagcttatcg ctcatgttcc gtatgccata gtagctgcgt ttattggaat
    14881 tgtatttgtg gtagtcaaca aaattgaccg cacaaaaaat gatatggtta tggatatgcc
    14941 ctttgtctat gtgggtagtt aagacgtatt catgctgtcc ctttgttacc gcgtcggcaa
    15001 gctgtttccc gatttcgtgg gctttctcat aatccacttc ccccggctca aaggactgta
    15061 tcaaatggtg ggcgagattg ttccctttct ctatggcttg tgcaagcgta aatccaaact
    15121 caatatctgc cgtttcatag ctgcacccaa aagaggacac aagcattttc ccgtctgttt
    15181 tatccgggtt ttggatatag tcaagggctt tgctcaacgt gcttttaata ggcttaatct
    15241 ttgtaaccgc catatctccg caagcacccc ctttatttcc tctatgtcct cttggtagac
    15301 gctgcctgtc gcgttgacac gctttgcaat ctgattgata ttgaccccga ttttctgtat
    15361 ctccgctgtc atggctttta tgtcgctatg gtctgtctga atgatatacc cgtcaatggc
    15421 aatcttgcga agatacgccg ccatgttccg ggtggggacg agcttcattt tctgctcaat
    15481 caatgccctt tcttcctctg ttacatagaa tttgatttgt atttttcttt tgcgtccgtt
    15541 catgcaatcg ctcctttccg ctttgagggt ttgggacact tcccaacaag caattttcaa
    15601 ccgacgcacc gcagggcggg agggcgaaaa tacggaaaat ggtactcttt tcctatgctt
    15661 gctacgaaag ttttttcctc actggttact acaacgcaat gctctatatg ccaaaaaaga
    15721 gcaaaagaaa aacgctgcaa tctgtaaaga ttacaacgct tcctaaatca tattcaattt
    15781 gccggacaca tttatttgtc ctctttttcg acttctgaaa tttcttttgc agttgccgtt
    15841 acaatccgta tgccattatc gctcatatta tcaaggagag cgtcgagctg ccgccgcttt
    15901 gtatcttttg gggcgggcgt atctaaaaga aactggtcta cggatatatt gtaacgaagc
    15961 ataagctcaa aaaagatttg caggctgggg tgctgtcctt tgttttcgat atttgcaaga
    16021 tagcgaggag aaatatacat ttcatcactt acctgtttgc ggctttcttt gcgccccgtc
    16081 cgcgcagctt ttatagcttc tccaaatgcc ttgaagtcat atttttctac cggtcttttt
    16141 gccatattta ttcacccagt tacattttac tgttcccctt tctccttgga tatgtttata
    16201 cagttccact aaatagccat aataattcat atttatatac ttgctattgt tcgtgtgtcc
    16261 gactataatt attatgataa gattttcaga aaggacgggt gttgttatgg aatatatgtc
    16321 ttgccccgaa gcagcaaaga agtggggaat ttctgaaagg cgggtacaga tactttgcag
    16381 ggagaaacgc atacagggtg tttcaaaact tgggtatatg tggttgatac caaaagacgc
    16441 ggaaaaaccg attgacggta gaaccaaaca aggaaaggag ctataccatg aataaagttt
    16501 tgattataga tgatgataag gaactttgtg cgctgattaa acgcagcgta ctatcagaag
    16561 atatagaagc tgatttttgt aatacgggaa aagagggatt acaaaaatta aaagagagag
    16621 aatatcaact tgtgatactg gacattatga tgcccggtat ggacggattt gaaacattgg
    16681 aagaaattag aaaagaaaac agcctgccga ttttgatgtt tacatcaaaa aatgacagtg
    16741 cttctaaagt acgcggttta agggcaggag ctgatgatta tttgacaaaa ccttttgaca
    16801 tggacgaact gatagcccgt atcatttccc ttatacgccg ttatacccgt ttcaatcaac
    16861 aggacggaat atcacagcaa cttgaatttg acgggttgaa aattgatatt gaaaatcgtt
    16921 ctgttactac ggaaaatgga acttttgaat tgcctccaaa agaatttgac ttactcttat
    16981 actgcgcgaa aaatcaaggg aaaattctga caaagcaaca gatttatgaa gaagtgtgga
    17041 gagaggaata tttctatgat gacagcaaca ttatggcgat tatcagccga cttcgcaaga
    17101 aattagaggt aaatcccgga agtccaaaat atattcaaac gattaaaggt atcggttatc
    17161 gttttaacaa ggaggtttga ttttctatgg aagcagtcat ttttctatcc ataataattg
    17221 cacttttctc tattcttgtt tcctgctttg ctattcgacg tgttaaaaaa cagatagccg
    17281 agataacaga cgcattgatt gatgtaaaaa acggaaatgg aaacaggcgt attttatctg
    17341 caacaaatga actggtagcc cctcttgcct atgaaataaa tgagattgtt gtgtcttatg
    17401 agagcaggct ttctactgtc cggcagacag aagaaacaaa ccgacagctt atgacaagtc
    17461 tttcccatga tgttcggaca ccgcttacaa cgcttattgg gtatcttgac gctgcacata
    17521 aaggaattgt tacaggaaaa gacagggacg actatatcga aacagcccgc cgcaaagctc
    17581 acgatttgaa agaatatatt gatgttcttt ttgactggtt caaattaaat tctaatgagt
    17641 ttgctatgga tataaatatt gttgaagctg cggagctgac acgaaatatc ttgattgact
    17701 ggatacccat atttgaagat aagcagatag attacaatat tgatattccc gaacagcctt
    17761 tccgggtaaa acttgatacg gacggctata tgcggatatt gaacaatctt attcagaatg
    17821 taatttctca cagtcatgcg gataagatag aaataatcct gtcaaagcag gaaaaaaata
    17881 tgcaaatccg attagcagat aatggaatag ggattgaaaa agaagatttg aagcatattt
    17941 ttgaacggct ctataaatgc gataagggac ggtctgaaaa aggcagcggt cttgggcttt
    18001 ctatcgcaca ccaacttgta gaaaaaatga acggcacaat aacagcaaat agcatacagg
    18061 gaacaggaac ggaatttact ttgctttttc ccttagcaaa ttaaggttct cccgtccttt
    18121 tgacgggagc cttaattatt tttgggacaa tatttcaaat tgcaaggtta atgcaaggtt
    18181 gcggcaaggt taatgcaagg ttggtatgat agaatacttt acagaacagg aggtttgaat
    18241 atggacaaaa attatatcat tgaaacaaaa aatcttacaa agcagtacgg ctcacaaaag
    18301 agtgtcgccg acttaaatat tcatgtgcag aaagggagaa tttacggact gcttggaaga
    18361 aacggagccg gaaaaacgac cactatgaaa atgctacttg gattgacaaa gccaacttcc
    18421 ggcgaagtta aaatatgggg aaagtctttg caggggaatg aaaagaaatt gttgccccgt
    18481 atcggtagtc tgattgagtc gcccggtttt taccctaatt tgactgctac ggaaaactta
    18541 caaatttttg ctaccttacg gggcataccc aaccgtcatt caattaaaaa cacattggat
    18601 ttggtaggat tgccttataa agacaaaaaa ctgttttccc agtattctct tggtatgaaa
    18661 caacggcttg cgattgcact tgcagttatg cacgaccctg aacttctgat tttagatgaa
    18721 cctatcaatg ggcttgaccc gattggaatt gcagaagtac gttcttttat tcgcgagctt
    18781 tgcgatacaa agggaaaaac aattttaatt tccagtcata tcctttcgga gatttctctg
    18841 ctagcagatg atattggaat tatcgaccat ggcacactat tggaagaaga aagtcttgcg
    18901 gaactggaac aaaaaagcag caaacatatc cgatttactc tttctgatac cgcacaggca
    18961 gcaagaattt tagagcgtac tttccatgag agccatttct ctatacagga cgaccataat
    19021 ttacgcttgc ataatcttga tttgtccgta ggaaaaatcg tgactgcttt tgtagaaaac
    19081 ggattggagg tatcagaagc ccatacttgt gaagaaagtc ttgaagatta cttcaagcgt
    19141 gtgacaggag gcgaaggaat tgcttaaact gattaagtgc gaattttgga aattaaagcg
    19201 aaaaagactg ttgtatgcac tgatgtcatt gtcgttattt tttccgttga ttttggcata
    19261 tatggcaaag gcgggaaccg gtgcagatac aacggaacac tatcttcaaa cccgatttga
    19321 ttatgtttat acaatgatgt tgggatatgg tttagttttt ttgcttccat gtctaattgg
    19381 tatcattgcc gctattttgt tttttataga aagagattgt gatacttcca aaaaccttag
    19441 aacaattcca gttacaaata cacaactcat tatggcaaaa atttctatgc tctttatttt
    19501 cagcgttgca ttttgtctga taagcacatt atctgttgcg ttgttttgta aattgtttca
    19561 tgtaggaatg gtttacggta tgacttataa gatatttatg agccttattt ttggagttct
    19621 tatcgtagca gcttctttac cgattgtatt tttaattatt tactttaaca aaagtttttt
    19681 actttctatc ctgcttgcat ttttttattc catttttaac tggggcatac tgggaacgat
    19741 tgggacatct ataagtgctg ccaaaattgc tttcttaaat tattttccgg ttatatgtgt
    19801 tatgaactgg acaagcggct taatgatgga taatttgcaa aaagataatc ttttgccgga
    19861 agcctatgcg attgtaccaa ccacttgcca tacaattttt attatggcaa ttacagttat
    19921 actttctttg tggctgataa ttcgctttta taaaaaatgg acgaggtaaa agatatgaga
    19981 aatataataa aaactgaatt tttaaaatta aaacgatatt ctgtaataaa agccggaatt
    20041 attatgacaa cactttctcc gttgctgtca ctgttttatt ccacagccaa cggtggacag
    20101 acatggacgt ttgactattt tatgcaacag gttatgatta gtaactgtac cttatttttc
    20161 ccgattatta ttgcgctgat tgcaggttat atcataacaa gggaatatac agacgatact
    20221 atgaagaata tattaacaat tcctattcca tataaacagt tactttctgg aaaattactt
    20281 attctattgc tgttgacaat cagctttagt cttataggtt gtgtgatagc attagtaatc
    20341 aatattattg tcggatttcc gggagttcat tttggcaatc tactcaatat gtttattcgt
    20401 gttactgggg cgaatatagg aatttatatt tcagtcttgc cgattattct gatattctgt
    20461 tgttcggcaa ataatttttt gggtggagtt gcactagctt ttgtgtatgg atattttgga
    20521 acttttgaag gaacactgtt aaattattat ccgattaaag caagcatgat tttagtagac
    20581 cctacttgtg gtacggaata tggttatact tatcacattt tccccgcttt cattacaata
    20641 gttttgactt tcttgatttc tatgattatt cttgcaaata aaaagaaaga acccggtaca
    20701 ttgactgttg gtaagaagaa aaaagcagtt agaaaaaagg gttggtaaat aatttgattg
    20761 atagtacctg ccgaacgacg gcaaaagaaa aaaagctgtc gcagagcaga tacactttca
    20821 cgcgcctttg aagcggtggc tttgattttt agagccgccg ctttttttgt ctactgctaa
    20881 atcacgacaa cctataacat aaattagcgg catttaggag ggctgccgct atggggattt
    20941 ctttgttgct atacagcata cataggttac attcattaaa aaaagtacag ttcccccacc
    21001 gggtaagtct ggctatgaat atgaactatc aatacatcta aatactgttt actattcctt
    21061 tgcgcctgtc cgcgcctttt tgcgggcagg cgcattttgc actttacaga agatttttca
    21121 aataaatttt ccagttcgtc cggcgtttga aaaagtcgtc tgtattatcc cgcgaaaagg
    21181 ggaggtacta cccgcctttc ggcagaaagg aggtgattac atggaaccta atcgcagaga
    21241 atttcagaag caatgtgcct ttcaaagttt ctgtaaacgg gtgctgcaca acgaagcctg
    21301 caacgcccat gatgaaatcc ggcgacgcag gaaacgggaa gtttcttttt gtgaccttgc
    21361 cttgcatgag gaacggcagc tttacactac ggataagtat tttcaagctg aaacagccga
    21421 gccgacttat caaatggcag gaaaagaaat aaccccgaag ttattgcttg aagcaatacg
    21481 caccctgccg gaagaaaaga gaactgccat acttctgtac tatttcgagg gcatgactga
    21541 tgtggaaatc ggaaagatgt tcaatacatc gcgcagcacg atacagtaca gacggacaag
    21601 ctcttttgaa ctgttaaaga aatatttgga ggaacacgct gatgaatgga acgaatggta
    21661 atcaaaacga ctacccggaa aatgcccttg ttccctatcc cgttattttg gcggcgagca
    21721 aaggcgaccc ggacgctatg aaaatggtct tgcagcattt cagcggctac atagcaagtc
    21781 tttccatgcg gaagctctac gacgagcgcg gcaatgtcta ttatggcgtt gacgaggata
    21841 ttcgggaacg actgcaagca agactgatga gggctatcct cacttttcgg gctgaataac
    21901 ctatgcgata ttggaacgct tctttcccct tttccgttcc ataccgcaag acagccccct
    21961 gctctttgac aaagaaagcg gcgcaagaaa acggcgacgc agcataaggc aaatcggata
    22021 cgttcctttt gtgccgagcc atacggcggg tgcgccatga cctttccccc tactggaaaa
    22081 gcgagcgaga accaccacgc cgcaaagcag atatagcaga ctgcgggcga taacgcacaa
    22141 aatacataat gatacttcct tacagccata gtccgagcgt tagaagcgtc gcaggcaatg
    22201 ggtagggctt cggcagaccg ccggaggggt gcaagtccca tgaggttgcg ctaacagccg
    22261 tccgattaaa gcctatgtta ctgattgact tttgacccat tgagaaaggg ggacgacaaa
    22321 atgaacaata acgacgtgcc gatttgggaa aaatacaccc ttaccataga agaagctgca
    22381 aaatatttcc gtatcggaga aagtaagctg cgtaaactgg cagaagaaaa tcccgtcccg
    22441 gactgggtaa tgatgaacgg aaaccgtatt cagattaagc gaaagcaatt tgaaaaaatg
    22501 attgatacgg tggacgcaat ctaatagtga aatcgagcct tgaatgtggt acaatgtagt
    22561 caagcatatc aaggctcttt ccattatgga aaggagcata acgatgtcgg aaaaaagacg
    22621 ggataataaa aatcgtattc tccgcaccgg agagagccag agaaaagacg gaagatatgc
    22681 ctacaaatat atagatacct ttggaaaacc gcagtttgtg tattcttgga agttagtacc
    22741 tacggacaaa accccggcag gaaagcgcga cgatatagcg ttgagggaaa aagagaaaga
    22801 aatccaaaaa gaccttgacg acggtatcga ccatatcgga aagaaaatga cagtctgcca
    22861 gctttacgca aagcagatac gccaccgggc aaatgtgagg catggtacaa agcaggggcg
    22921 aaaacagctc atgcggattt tgcaggagga taaacttgga gcctgccgga ttgaaaatgt
    22981 aaagctctcc gacgcaaagg aatgggcatt acgcatgaaa gaaaaaggtt atggcttcaa
    23041 gactatcaac aaccacaagc gttccttaaa ggctgccttt tacacagcca tacaggacga
    23101 ttgtattcgt aaaaatccgt ttgacttcca gttgaacaca gtccttgaag atgatacgga
    23161 accaaaggaa cctctatccc ctacgcagga agcggctttc ctgtcctttg tgcagcatga
    23221 taaagtctat cagaaatact acgacgagat tatcatactg ttagggacag ggcttcgcat
    23281 ttcggaactc tgcggattga cggaagctga cattgactta gataaacagc ttatcaatgt
    23341 agaccaccaa cttttgaaga ttgcagacgt gggctactat gtagaaacgc ctaagactaa
    23401 aagcggcaac cggattattc ctatgagtga aaaagtgttg gaagcatttc agcgggtgct
    23461 gaacaaacgg aaatatacac agcctgttat tttagagggc tacacaaagt ttctgttcct
    23521 taaccggaac ggtttgccaa aagtggcagt caactatgaa agtatgttcc gggggcttgt
    23581 aaggaaatac aacaaaacgc aaaaggtagc attgccaaag gtaatgacac cgcacacctt
    23641 acgccacaca ttctgcacca cgttagcaaa tgcgggaatg aaccccaaag cattgcagta
    23701 catcatggga cattccaaca tcaatatgac gctgaactat tacgcacaca caacttccga
    23761 aacgtcaata acagaaatga agcggctgat tgcatagtag taaatggaga atttactact
    23821 ccacatacta cttttgaaca ccaaaacata cggggatata agaagatttg agaatatctt
    23881 ccatgacgaa aaatgccgga aaagcctgta acaataggct ataccggcat ataagaactt
    23941 ttgaaaagat agtcaaaaat tactttgtga tttataatta aacatgttag ctattttaga
    24001 tgctacccta ctttaatcta attaattgaa ataaattaaa tattttctat atcagcaata
    24061 actagagtca acaattaaaa tactaatatt gacaattgaa acactgatac taacaagcaa
    24121 aacactaata ttgacaattg aaatat
//
LOCUS       DBGJFF010000344        12629 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.guided.113, whole genome shotgun sequence.
ACCESSION   DBGJFF010000344 DBGJFF010000000
VERSION     DBGJFF010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12629
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.guided.113"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            16..417
                     /locus_tag="ACOIAF_004019"
     CDS             16..417
                     /locus_tag="ACOIAF_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015567060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP protein"
                     /protein_id="HGZ2670942.1"
                     /translation="MDARELTRDEKKKIRTLVTGMCANYDRESGLCLPLDCACYMLHK
                     CWTGAYCRYFREAVLPLDPELQAALTTEGISPELRACAVCGKAFWPEGRQAYCSDACE
                     AEGNRRKSRERMRKMREKRPGGGYDLPPPKA"
     gene            550..1140
                     /locus_tag="ACOIAF_004020"
     CDS             550..1140
                     /locus_tag="ACOIAF_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670943.1"
                     /translation="METVQGYVILKAATFETGHGFALGHNPGAPSPFVTWQFTEGENG
                     HRDYYWGRYGTSQAWAQRDFDRRVDDYQQLYHAAVKHTELGPEGVYRYYSTQRPVDIG
                     TYPKLPDNQPLSIVNYDDDRRRPVADSRLMAWGELTYAKPLTEKQMEDYELKPAPGNP
                     DRVRPSITARLKEGTRGQEPPKEPGPKRSHKTREER"
     gene            1153..1335
                     /locus_tag="ACOIAF_004021"
     CDS             1153..1335
                     /locus_tag="ACOIAF_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003500078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4316 domain-containing protein"
                     /protein_id="HGZ2670944.1"
                     /translation="MHEKDNYLKTAELSTEQNCNMIDGVPNNTPIQPTPPELDAKPLD
                     KVKEPKERRKGRELER"
     gene            1335..1676
                     /gene="mobC"
                     /locus_tag="ACOIAF_004022"
     CDS             1335..1676
                     /gene="mobC"
                     /locus_tag="ACOIAF_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HGZ2670945.1"
                     /translation="MENRKRNVHLHVMVTPDELAAIHERMAEAGISNAGAYVRKMALN
                     GYILHIDLAPVKELISLQRRCSNNLNQVAIHAHTYGVYPEEIDGLKRDYEKLWGEVSK
                     VLRELSELVAK"
     gene            1852..2142
                     /locus_tag="ACOIAF_004023"
     CDS             1852..2142
                     /locus_tag="ACOIAF_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005927606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1845 family protein"
                     /protein_id="HGZ2670946.1"
                     /translation="MKILLKILIAPFALALSLLAALLVFLFDICAVLLTIASVILAVL
                     GVALFFTPTPIGGIVFLFLAFLLSPYGLQAAAGSLLWALDGGKSALYRFLAS"
     gene            2222..3637
                     /locus_tag="ACOIAF_004024"
     CDS             2222..3637
                     /locus_tag="ACOIAF_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008401432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HGZ2670947.1"
                     /translation="MATTTLLQRHAGEGETIAEAIRDCLDYGKDPEKTESGKYISAYE
                     CDPATVADEFLLAKASYAAMTGREQKKENNVLCYQIRQSFYPGEITPKEANRIGYELA
                     MRWTKGRHAFIVTTHTDKQHIHCHIYYNSTTLDCTRKFRNFWGSSFALRRLSDRLCLE
                     NGLSIVENPKPRSKGKYRNYGEWQKERKGPLSYQDRLRLAIDTVLAEHPADLDEFLNL
                     MKRAGYEVKTVRGGGISFRLTGQGQERFTRLRASTLGDGYDLQDVLAAIEGKEKRPGH
                     SERKISLAVDIQAKLAAGKGPGYERWAKVFNIKQMAAALAYIQDNGLTDYEQLAQKAT
                     EAADRFHAISEQIKQTEQAMKTNAGLKAATVQYAKTRPVFEQYKATKYSRKFLTEHEA
                     DLELYRAAQAEMRSLLGGAKLPKMDVLKVEGRKLTAKKKQLYGEYQKARRDMQEIVTI
                     KANIDTLLGYTEPGRKHEKER"
     gene            complement(3807..4172)
                     /locus_tag="ACOIAF_004025"
     CDS             complement(3807..4172)
                     /locus_tag="ACOIAF_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005930876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGZ2670948.1"
                     /translation="MRMNQDERRFDFHGLGAALKRAREEKGWTQAYVAELVDRDSRTI
                     MNIENKGQYPSFDLFVKLITMFDVSVDQFIHADGGARSSSCRKQIDVLLNSMNEKELV
                     VIEATAEGIKKARETEVPE"
     gene            complement(4275..5645)
                     /locus_tag="ACOIAF_004026"
     CDS             complement(4275..5645)
                     /locus_tag="ACOIAF_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005927602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HGZ2670949.1"
                     /translation="MNKSERFFRRYIFSIVSIIILFLFVNILLVASFFAAAYLGNVKN
                     SNFPIEDFSNHITETNGQFNADIQAEDMLNNACAWAMILDENGNIIWEMNVPEELPRH
                     YSTTDVAMFSRWYLNSYPVNIWNRQDSLLVVGFPQGYVTNYYTSIISQYVRPIAFGFL
                     AAVCINVLLMLYLFVRNAHRIEKAMEPILNGIRHLSSGHPVHLEENGELAEINAGLNK
                     AGDYLLKKDNTRAEWIRGISHDIRTPLSMILGYASEIEETSSLPETTRKQAEIIRKHS
                     EKLKNLVENLNLTTKLEYSMQPMQKQTLVPVELARQAVSEVLNDGLSDKYELELSEDN
                     PGKAITIIGDQSLLNRMLCNLIRNCIVHNPNGCRIQVSVGSCDRTCTFSVIDNGCGIS
                     EPQLNTLNNDKDISSTQKQTGEIEHGLGLKIVRQIVKIHQGTIQFSDTAPHGLSVEVV
                     IPIEIY"
     gene            complement(5642..6337)
                     /locus_tag="ACOIAF_004027"
     CDS             complement(5642..6337)
                     /locus_tag="ACOIAF_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGZ2670950.1"
                     /translation="MNDKRILVVDDETDLLEMVKSIFTRAGFTQVLTASSGEAALKMC
                     EEQEPDMMILDVMMPGMDGFAALKELRKTSKIPVLMLTARGEAEDKFVGFENGADDYL
                     LKPFLPKELLFRVQAILKRVYPEKERKVFFDAATVDLEKAIVQRSGENISLTAKEFQL
                     FEKLYENQGRIVTTGALCQAICGDFWQGYESTLATHIRHLREKIEENPSKPVALVTIK
                     GIGYRLNIKGVQT"
     gene            6597..7772
                     /locus_tag="ACOIAF_004028"
     CDS             6597..7772
                     /locus_tag="ACOIAF_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HGZ2670951.1"
                     /translation="MIYVGIDVAKDKHDCFITNSDGEVLFKAFTIKNNLDGFDELYQK
                     IESVMEDASKVKVGLEATGHYSYNLLGYLLDKGLATFVINPLHTNLYRKSLSLRQTKT
                     DKVDAHTIASMLMSDVNLKSYSDTSYHNEELKSLTRYRFDKVKERAKLKTSISRLVCI
                     LFPELEKLVPTLHQNSVYELLYEFPGAKQVANAHLTRLSNLLETASKGHYTKETSIAF
                     REAARTSIGSNMPAKSLELKHTIKLIRELDSEIEEIENEIKVIMDEINSPILSIPGIS
                     YRMGAMIIAEIGDFSQFDSPDKILAYAGMSPSTYQSGQLDNCYARMEKRGSRYLRYAL
                     FNATAYVCLWDPTYKAYLAKKRAEGKHYYVAMSHATKKLVRLIYHLERTGQQYQKAI"
     gene            8078..9001
                     /locus_tag="ACOIAF_004029"
     CDS             8078..9001
                     /locus_tag="ACOIAF_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2670952.1"
                     /translation="MNDYIIETHNLTKKYGSQASVSHLNIHVKKGRIYGLLGRNGAGK
                     TTTMRMLLGLTAPTSGEVTIFGKPFQGNEKNILPRIGCLIESPGFYPNLTGTENLKIF
                     AELRGLRSPQYIKNALELVNLPYRDKKLFSQYSLGMKQRLAIALAVMHNPELLILDEP
                     INGLDPIGIAEVRDFIRELCDTSGKTILISSHILSEISLLADDVGIIDHGKLLEEESL
                     KELEAKNAKFIHFCVSDAQKAAKIIAATFHSKDFRLTDANNLYLYDMTLPVERINRSF
                     VESGIEIQEVHLCEDTLEDYFKKITGGEGIA"
     gene            8985..9797
                     /locus_tag="ACOIAF_004030"
     CDS             8985..9797
                     /locus_tag="ACOIAF_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006874368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2670953.1"
                     /translation="MRGLLNLIRCEFWKLKRKMFVQLVLAASFLFPIPLTAIIYYLNT
                     AQNKYATKAAAFDALWQSVIGFGMLLLLPCILGIVAALLFFMERDNDTFKNLRTIPIT
                     SSQMVIAKCIVLLLLSIAFCISSTVASILCGFVFFGVTDILFKVLFSAVYGLLIAISA
                     LPLILLIVFFSRSYIFSIMLCVFYSVFNLLSTFSMTALPKWLVLTLSTPSIMLWGTQQ
                     MSSRTAINDTEDLQNFIQMGLIPSTPQLIITLGVIGAVSLLLAVYLYKKRSE"
     gene            9799..10569
                     /locus_tag="ACOIAF_004031"
     CDS             9799..10569
                     /locus_tag="ACOIAF_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009255077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2670954.1"
                     /translation="MVTIIKTEFMKLKRYFIVWIGVSLMLLTVLLTLFTTMADDRMVW
                     DYQFLFEQVVKHFVTMIFPMCITLISGYMISREYTDDTLKNIETLPISFRKLLAGKLI
                     VSAILSFFLGIVCFAFTVVANFIMGYDGFALIPALTGLVQMALLGFFLYLTMLPIIVL
                     TSRYKGSFLVGVIVAFVYGFIGMFANGTLQSIYPVSAALGLINYRAGAEGVMWNKGLC
                     FISILIMCAIGIALMFVKQKPEKREAKKTQHTAPKKGW"
     gene            10590..10937
                     /locus_tag="ACOIAF_004032"
     CDS             10590..10937
                     /locus_tag="ACOIAF_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005927589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HGZ2670955.1"
                     /translation="MSSKKKIGIVIVVAVLAITFMTVAFLTTQTNTDYYTQIDNRRVT
                     DIAPHGAMNYKYTLVAYDESGNAKDVTFETSKVLTDGAYLCLKVAPIRGVVTWAEMQF
                     DELPSSVQEKYKE"
     gene            11434..11856
                     /locus_tag="ACOIAF_004033"
     CDS             11434..11856
                     /locus_tag="ACOIAF_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HGZ2670956.1"
                     /translation="MAIINLRDYYPFYTSDCFMEVSEEVAEMFKEFDRKEAAYRLRTY
                     RHKAYYSLDRDDGLEHEAVFVALSPHELYERKVTMQELHAAIASLPDKQAKRIYAHFI
                     LGMTKQDIARAEGVHEKVVRVAIERGLRRLEKILKNSL"
     gene            12298..12567
                     /locus_tag="ACOIAF_004034"
     CDS             12298..12567
                     /locus_tag="ACOIAF_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015564338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670957.1"
                     /translation="MLSDDRPTDPAWLPIFSKKEHFMGNAFGMIPGLEPDEWSNDACR
                     GYVIMAMEDCGFSKKDIRRVVGQLYEVFDLNSVEDAKEKYHSSPY"
BASE COUNT         3403 a         2847 c         2960 g         3419 t
ORIGIN      
        1 ggcccgaagt cttaaatgga cgcccgggag ctgacccgtg acgagaagaa aaaaatccgc
       61 actctggtca cggggatgtg cgccaactat gaccgggaaa gcggtctgtg ccttcctctg
      121 gactgtgcct gctatatgct gcacaagtgc tggacagggg cgtactgccg ttacttccgc
      181 gaggctgtcc tgccccttga cccggagctt caagcggcgc tgacaacgga aggcatctcc
      241 ccggagctgc gggcctgtgc ggtctgcgga aaggcatttt ggcccgaggg gcgtcaagcc
      301 tactgttccg acgcctgcga ggctgagggg aaccgccgga aaagccggga acgcatgagg
      361 aaaatgcggg agaaaaggcc gggcggcggt tacgatttgc cgcccccaaa ggcttgatat
      421 tccgggcttt tttgagggcg tttccggggt gggaatatct tattacattc ctccccggtc
      481 tgccctctta tttcgtaaca tccagcactt cgggccatgc tggcccgagg cacagaaagg
      541 agggatctct tggagacagt acaaggatat gtgattttga aagctgccac ctttgaaacc
      601 ggacacggct ttgcgctggg gcataatccg ggagcgccga gcccctttgt gacctggcag
      661 tttaccgagg gggaaaacgg ccatcgggat tactactggg gccgctatgg aaccagtcag
      721 gcgtgggcgc agagggactt tgaccgccgg gtggacgact atcagcagct ttaccatgcg
      781 gcggtcaagc ataccgagct gggaccggag ggcgtttacc gctattattc cacccagcgc
      841 cccgtagaca taggcaccta cccgaagctg ccggataacc agcctctttc catcgtcaac
      901 tacgatgacg acaggcggcg tcctgtggca gacagcaggc taatggcatg gggcgaactg
      961 acctacgcaa agccgctgac cgaaaagcag atggaggatt atgaactgaa accagcaccg
     1021 ggcaatcctg accgggtgcg cccatccatt accgcccggc tcaaagaagg aaccagaggg
     1081 caggaacccc cgaaggagcc cggcccgaag cggagccata aaacccgtga ggaacgataa
     1141 aggaggatcg ccatgcacga aaaagacaac tatctgaaaa ccgccgagct ctccacggag
     1201 cagaactgca acatgatcga cggcgtaccc aacaacacgc ccatccaacc cacgcccccg
     1261 gagctggacg caaaaccgct tgataaggta aaggaaccca aagagcgccg gaaaggccgg
     1321 gagctggaac gctgatggag aaccgcaagc ggaatgtcca tctgcacgtc atggtaacgc
     1381 cggacgagct ggcagccatc catgaacgga tggctgaggc gggcatttcc aacgccgggg
     1441 cttatgtacg gaaaatggct ctgaatgggt atatcctgca catcgacctt gcgcccgtaa
     1501 aagagctgat ctctctgcaa cggcgctgtt ctaacaatct caatcaggtt gccatacacg
     1561 cacataccta tggcgtatac ccggaggaaa tcgacggatt gaagcgggac tatgaaaagc
     1621 tgtggggcga ggtgtcaaag gtgctgcggg agctctccga gctggtggca aagtaagacg
     1681 agggcggcag gcttccgttg cagggcttgc cgccctgttc tttttttacc acttcgggcc
     1741 agtatgaccc gaaggtttgg gacaggattt actaatcttt cgcaacattt atgtccccgg
     1801 gcaggcatga taaaatagag gtagaaagga tagataaagg aggttttgaa gatgaagata
     1861 ctgctgaaaa tattgatcgc tccctttgct ttagcgttgt cccttctggc ggctctgctg
     1921 gtgttcctgt ttgatatttg tgccgtcctg ctgacgattg cctctgtgat cctggcggtg
     1981 ctgggtgtcg ctcttttttt cacgccgacg cccataggag ggattgtatt tctgtttctc
     2041 gccttccttc tttcgccgta tggactgcaa gcggcggcgg gctcccttct ttgggcgctg
     2101 gacggaggca aatccgctct gtaccggttt ctggcaagtt aagcagcctc gggccatact
     2161 ggcccgaagt cggggcggtc tgaatgggcc gcccttttct catggaaagg agggatgatt
     2221 tttggctacc acaactttgt tacagcgcca tgcgggcgaa ggcgaaacga ttgctgaggc
     2281 tatccgggat tgtctggact atggcaagga cccggagaaa acagaaagcg gaaagtatat
     2341 ctccgcttat gaatgtgatc cggccaccgt ggcggacgag ttccttttgg caaaggccag
     2401 ctatgccgct atgacgggcc gggaacagaa gaaagaaaat aatgtgctgt gctatcagat
     2461 acgacaatcc ttctatccgg gcgagatcac cccgaaggag gcgaaccgta tcggctatga
     2521 gctggctatg cgctggacaa aggggcggca cgcttttatc gttaccacgc acaccgataa
     2581 gcagcacatc cattgtcaca tttattacaa ctccaccacc cttgactgca cccggaaatt
     2641 ccgaaacttt tggggctcca gcttcgccct tcggcggctc tctgacaggc tgtgccttga
     2701 aaacgggctg tccatcgtgg agaacccgaa gccccggagc aagggtaagt atcggaatta
     2761 tggagaatgg cagaaagaac gaaaagggcc gctttcctat caggacaggc tgcgcctcgc
     2821 cattgatact gtgctggcgg agcaccccgc tgatctggac gaatttctca atctgatgaa
     2881 gcgggccgga tatgaggtca agacggttcg gggcggcggt atcagtttcc ggctgaccgg
     2941 gcagggacaa gaacgcttta cccgtctgcg ggcctccacg ctgggggacg gctacgactt
     3001 gcaagatgtt ctggccgcca ttgagggcaa agaaaaacgc cccggacatt cggagcggaa
     3061 aatcagtctg gcggtggata ttcaagcaaa actggctgcc ggtaagggac cgggatatga
     3121 acgctgggcg aaggtattta acattaagca gatggccgct gcccttgcct atatacagga
     3181 caatggactg accgattatg agcagttggc acagaaagcc accgaggcgg cagaccgttt
     3241 ccatgccatt tccgagcaga tcaagcagac ggaacaggcc atgaaaacca atgccgggct
     3301 aaaggccgca acggttcagt atgccaaaac ccgtccggtc tttgagcagt ataaggcgac
     3361 gaagtatagc cggaaatttc ttacggagca tgaagccgac cttgaactgt accgggctgc
     3421 acaagcggaa atgcgctctc tgttgggcgg ggcgaagctc cccaaaatgg atgtgctgaa
     3481 ggtagaaggc cgtaagctca cagcaaagaa aaaacaactc tatggagaat atcaaaaggc
     3541 acgacgggat atgcaggaaa tcgtcacaat caaggcgaac attgatactc tgctgggcta
     3601 caccgagccg ggcagaaagc atgaaaagga gcgttgagat tatgaaaaca aggagcaaag
     3661 cgacgctgac acttcgggcc atgttggccc gaagatacgg gtttggggcg agccccaaca
     3721 agccgccttt gtgccacttg tggccacaga ggcattgctt gccacttagc ggcagcccct
     3781 aaaatggcgc aaaaaaacag aggcccttat tctggaacct ccgtttctct ggctttctta
     3841 ataccctcgg ctgtggcttc tatcacgaca agctcttttt cattcatgga gtttagcagc
     3901 acatcaattt gctttctgca agagcttgat cttgcccctc cgtccgcatg aataaactgg
     3961 tcaactgaaa cgtcaaacat ggtaatgagt ttaacaaaaa ggtcgaagct gggatactga
     4021 cctttgttct caatattcat aatggtacgg gagtcacggt ctactaattc cgcaacatag
     4081 gcttgtgtcc agcccttttc ttctctggct cgtttgagag ccgccccgag gccgtgaaag
     4141 tcaaaccttc tttcatcttg gttcattctc atatcaccct atatcattgt acatttcggg
     4201 ttgaattatg agaatgtaat gaaatttgat gtgaggtagt attttatttc ttgttttcgt
     4261 ctgtctatta taagttaata tatttctatt ggaataacaa cttccacgct taatccgtgc
     4321 ggggcagtat cagaaaattg aattgtgcct tgatgtattt tcacaatctg ccgcacgatt
     4381 ttaagaccca gcccatgctc gatttctccc gtttgctttt gcgtactgga aatatctttg
     4441 tcgttattca atgtgtttag ttgcggctca cttattccac agccattgtc tattacagaa
     4501 aaggtacatg ttctatcaca gcttcctact gatacttgta tcctacaccc gtttggatta
     4561 tggacaatac aattcctgat taaattgcat aacatccggt ttagtaagga ttgatcgcca
     4621 ataatagtga tagcttttcc gggattatct tctgatagct ctaattcata tttgtctgat
     4681 aggccatcat tcaaaacttc acttacggct tgcctcgcca attctaccgg gacaagtgtt
     4741 tgcttttgca taggctgcat ggaatattcc agttttgtag tcaaattcaa attctcaact
     4801 aaattcttta gtttttcgct atgtttgcgg ataatttcag cttgctttct ggtggtttcc
     4861 ggcaaagaag aagtttcttc aatttcgctg gcatacccta aaatcataga aagcggcgtg
     4921 cgtatatcgt gagaaatacc cctgatccat tcggcgcggg tgttatcttt ttttagtaag
     4981 taatcaccgg ctttattcaa accggcattg atttctgcta actccccgtt ttcttccaga
     5041 tgaacgggat gcccggatga aagatgtcgt atgccattca aaatcggctc catagctttt
     5101 tctatccggt gggcatttcg gacaaataga tacaacatta acaaaacatt gatacagaca
     5161 gcagctaaaa atccaaacgc tatcggccga acatattgac ttatgatgga agtgtagtaa
     5221 ttcgttacat atccctgcgg gaaaccaaca actaaaaggc tgtcttgtcg attccaaata
     5281 ttaactggat aactgttaag ataccaacgg ctgaacatag caacgtccgt tgtggaatag
     5341 tggcgcggca attcttccgg cacattcatt tcccaaatta tgtttccatt ttcatccaaa
     5401 atcatggccc atgcgcaagc attgtttaac atgtcctctg cctgtatatc ggcattgaat
     5461 tgcccgttag tttctgtgat atgatttgaa aaatcttcaa tcgggaaatt tgagtttttt
     5521 acattcccta aataggcggc tgcaaagaaa gaggctacta acaaaatatt gacgaataga
     5581 aagagaatga tgatactgac tattgaaaat atatagcggc gaaaaaatct ctctgatttg
     5641 ttcatgtttg caccccctta atattcagac gatacccaat tcctttaatt gttacaagcg
     5701 caactggttt tgaaggattt tcctcgattt tttctcttaa atgtcggata tgagttgcta
     5761 atgtactttc gtatccttgc cagaaatcac cgcatattgc ttgacataat gcgcctgtgg
     5821 taacgatcct gccttgattt tcatacagtt tttcaaaaag ttgaaactct ttggctgtca
     5881 gcgaaatatt ttctccgctt ctctggacga ttgctttttc cagatcaacc gtagcagcgt
     5941 caaagaacac ttttcgctct ttctccgggt aaacgcgttt caatatagcc tgtactcgaa
     6001 atagtaattc ttttggtaag aatggtttta acagatagtc gtctgcacca ttttcaaagc
     6061 ctacaaattt atcttcggcc tcgccacgag ctgttagcat taagacagga attttgctgg
     6121 tctttcgtag ttcttttaat gcagcaaacc catccatacc aggcatcata acatcgagta
     6181 tcatcatatc tggttcttgt tcctcacaca tttttaaggc tgcctcgcca gacgaagccg
     6241 tcaatacttg tgtaaatcct gctcttgtaa aaatagattt taccatctcc aacaaatctg
     6301 tttcatcatc aactacaagg attcttttat cgttcataag gcaccaccta ctattatata
     6361 aagtcatagt ctttctttac cttttctaca ggtttgacta tgctatttta agatactgtg
     6421 gattggttat cacctcctac cacttgatgg tttaaaaaag gctccaacca cagtctcttt
     6481 gtttatttgt taatcagtta gttaccgcat gacggtttat caagaagtag gttacgattg
     6541 caaggagccc tgtggatctt aagacagtat caatacttta tatcaggagg tcctatatga
     6601 tttacgtagg aattgatgtc gcaaaagata agcatgattg ctttatcaca aactctgatg
     6661 gcgaagtcct tttcaaggct tttaccatca aaaacaatct cgatgggttc gacgagcttt
     6721 atcagaaaat agaatccgtt atggaagatg cttctaaagt aaaagtaggc ctagaagcca
     6781 ctggacacta tagttacaat cttctcggat atctgcttga taaaggtctg gccacctttg
     6841 ttatcaaccc gttacatact aatctgtaca gaaaaagtct aagccttaga cagacgaaaa
     6901 cggataaagt tgatgcccat acaattgctt ctatgctaat gtctgacgtg aacttaaagt
     6961 cctactcaga cacatcgtat cacaacgaag agcttaagtc acttactcgc tatcgttttg
     7021 ataaagttaa agaacgcgcg aagcttaaaa catctatatc ccgtcttgta tgtatccttt
     7081 tccctgagtt agaaaagctt gttccaacac ttcatcagaa ttctgtttat gagttactct
     7141 acgaatttcc tggtgcaaaa caggtagcta atgcacatct cacaagactt tcaaatcttc
     7201 ttgaaaccgc atctaaaggc cactacacaa aagaaacctc tatcgctttt agagaggctg
     7261 caagaacctc tatcggttca aatatgccag ctaaatcgct tgaattaaag cacaccatta
     7321 agctcattag agagctagat tctgaaatcg aagagattga aaacgagatt aaagtcatca
     7381 tggatgaaat caattctcca atccttagca ttcctggaat cagctatcga atgggtgcca
     7441 tgattattgc tgaaataggt gactttagcc aattcgactc tccagataag atccttgctt
     7501 atgcaggaat gtcgccttct acctatcaat ccggccagtt agataactgt tatgccagaa
     7561 tggaaaaacg tggttctaga taccttagat atgctctgtt taatgcaacc gcatatgttt
     7621 gtctatggga tccaacctac aaggcttatc ttgccaagaa acgagctgaa ggcaagcatt
     7681 actatgttgc aatgtctcac gcgaccaaga aactagttcg gctaatttat catctcgaac
     7741 gtactggaca gcaataccaa aaagcaatct aattcttcca acatcatatc ttcttgtcag
     7801 agcatcatac tcgatgctct atttgtcatg caattttcaa ggtactgatt gcgatgaata
     7861 agtcatcgcc actatactca aaacatttct gtttttgaaa ctatttattg actttcttta
     7921 atagttagtc ttttatttgt tgctcttatt atacctcaag ataggtaaaa tgcctattct
     7981 gaccttgaaa tctcaaagta aactcaaggt tggctcaatg ttatctcaaa gttgatatgt
     8041 tatgctgagt acacaaacaa aaaaggaggc ataagtgatg aacgattaca ttattgaaac
     8101 acataatctg acaaaaaagt atggaagtca ggccagtgtt tcccatctga atattcatgt
     8161 gaaaaaggga cgtatctacg ggctattggg acgcaacggt gcaggtaaga ccacgaccat
     8221 gcggatgttg ctgggactga cggctcccac gtctggcgag gtaacgatat ttggaaagcc
     8281 ctttcagggc aacgaaaaga atatcctgcc ccgtataggc tgtctgattg agtcgccggg
     8341 cttttacccc aatttgacag gaacggaaaa cctaaaaatc tttgctgaac ttcgaggatt
     8401 aaggagcccc caatatatca aaaatgcatt ggaacttgtt aatctacctt accgggacaa
     8461 gaagttgttt tcccaatatt ctcttggtat gaagcagcgg ttggcgattg ctctggccgt
     8521 catgcataac ccggaacttt tgattttgga cgaaccgatt aacggcctcg accccatcgg
     8581 cattgcggaa gtgcgagatt ttatccgaga attgtgtgat accagcggta aaaccatttt
     8641 aatttccagt catattcttt cggaaatctc tctgcttgca gacgatgtgg gaattattga
     8701 ccacggcaag ttgctagaag aagaaagtct gaaagaattg gaggcgaaaa acgccaagtt
     8761 catccacttc tgcgtatcgg atgcacaaaa agctgcgaaa attattgctg caacatttca
     8821 cagcaaagat tttcgcctta cagatgccaa taatctgtat ctctacgata tgactctgcc
     8881 agtggaaagg attaaccgtt cttttgtcga atcaggaatt gagattcaag aagtccatct
     8941 ttgtgaggat actttagagg actacttcaa aaagataaca gggggtgagg ggattgctta
     9001 acttgattcg gtgcgagttt tggaaactga aaagaaaaat gttcgtgcag ttggtattgg
     9061 cggcatcttt tctgtttcca atcccgctaa ctgccattat ctattatctg aacacggcgc
     9121 agaacaaata cgctacaaaa gcagcagctt ttgacgccct gtggcaatcc gtaatcggtt
     9181 ttggtatgtt gctacttctt ccctgcattt taggaattgt tgcagcactc ctctttttta
     9241 tggagcgaga taacgacacc tttaagaacc tacggacaat tccgataaca agttcacaga
     9301 tggttatcgc aaagtgcatt gttttattat tgcttagtat tgcgttttgt atatcttcga
     9361 ctgtggcctc tatcctttgc ggttttgtct ttttcggagt gaccgatatt ttgtttaagg
     9421 tattgttttc cgctgtttac ggattattga ttgcgattag tgcattaccg ctgatattgc
     9481 tgattgtatt tttcagcaga tcctatattt tttccattat gctttgtgtg ttttatagtg
     9541 tattcaacct gctctcaaca ttttctatga cagcacttcc aaaatggctt gtacttactc
     9601 tgtctacacc gagcattatg ttgtggggaa cccagcaaat gtcttctcgt actgctataa
     9661 acgacacaga ggatttgcag aacttcattc aaatgggatt gataccttct actcctcaac
     9721 taattataac tttgggggta attggggctg tttcacttct tctggcggtc tatctttata
     9781 agaaaaggag tgagtgaaat ggtaacaatt ataaaaaccg agtttatgaa gctgaaaaga
     9841 tattttattg tctggattgg cgtgtccctc atgcttctta cagttctgct aaccttgttt
     9901 actacaatgg ccgatgacag aatggtatgg gactaccaat ttctttttga acaggtagtt
     9961 aaacattttg taacaatgat tttcccaatg tgtatcacgc tgattagtgg gtatatgatt
    10021 tcccgtgaat atacagacga cacattaaaa aatattgaaa cattacccat ttcatttagg
    10081 aaacttctgg cgggtaaatt gattgtttct gcaattctgt cgtttttctt aggaattgtg
    10141 tgctttgctt tcacagtcgt tgcaaatttt atcatgggat atgatggatt tgctttgatt
    10201 cctgcgctta caggattggt gcaaatggca ttgttaggat tcttccttta cttgacaatg
    10261 ctgcccatta ttgttctgac aagcagatat aaagggagtt tccttgtagg ggttatcgtc
    10321 gcttttgtat atgggtttat aggaatgttt gcaaatggga ctttacaatc tatatatcct
    10381 gtctccgctg cgcttggttt aatcaattat cgagctggcg cagaaggagt tatgtggaat
    10441 aaaggactgt gttttattag cattctgatt atgtgtgcta ttggaattgc tttgatgttt
    10501 gtaaaacaga aaccagaaaa gagagaagct aaaaaaacac agcacacagc acctaaaaag
    10561 ggctggtaaa agaaagagag gtaaccttta tgagttctaa gaaaaaaata ggaattgtga
    10621 ttgtcgttgc tgtactagca attactttta tgactgtagc ttttctcact acgcaaacca
    10681 atacagacta ctatacgcag attgataacc gacgggtaac agatattgca ccgcatggtg
    10741 cgatgaacta taaatacaca ttggttgctt atgatgaaag tggtaatgcg aaagacgtta
    10801 cttttgaaac aagcaaagtt ctcacagatg gtgcgtatct gtgcttgaaa gtggctccca
    10861 tcagaggggt tgtaacatgg gctgaaatgc agtttgatga attaccttct tctgttcaag
    10921 aaaaatataa agaataaccc cgcaggacaa taaaggtgcc tgtaatgaaa tatatttaga
    10981 tcagctgcca cacgacggca aaagaaaaaa agccgtcgta cagcagccct tttgacacgc
    11041 ccatacgacc agcggcggct ttgattttca gagccgccgt ttttttcgtt tatcctaaac
    11101 caacgacgac ctaacaccgt gtaaatccac ggcggcatat aaggaggatt gccgccgtgt
    11161 ctatttttgc ctcttttcat agtacccatg acctacatca attaaaaaaa gcatagtgcc
    11221 ccctccgggt tattccggct atgtatgcga aatttgttgg aaccttaact attatgtcat
    11281 ttcatgcgtt cgtgcggctt catgccgccc gggcgctttt atgttcttat ggggctgctt
    11341 cgggtcatgt tggcccgaag tcattccccg gtgccgctcc ccgccgcctt cgtttcggtc
    11401 actcgtcaac caacaaccga aacggaggtc aatatggcta ttatcaattt gcgggactat
    11461 tacccattct atacatcgga ttgcttcatg gaagtatcgg aagaagttgc agaaatgttc
    11521 aaagagtttg atcgtaaaga ggctgcctac cggctgcgta cataccgcca caaagcctac
    11581 tattcccttg atcgggatga cgggctggag catgaagctg tctttgttgc cttatctcca
    11641 cacgaactgt atgagcggaa agtgaccatg caggaacttc acgcagcgat tgccagtctg
    11701 ccggacaaac aggcaaaacg gatttacgcc catttcattc tcggcatgac caaacaggac
    11761 attgcccggg cagagggcgt ccatgaaaaa gtggttcgtg tcgcaatcga gcgaggtctt
    11821 cggcgcttag aaaaaatttt gaaaaattct ttgtaatgcg taccgattta ggccggaaaa
    11881 tgaaatggtt tatgagaggc aaaacacttc gggtcacaat ggcccgagtt tcagacaagc
    11941 ctcatgcaga ttgaaaattg aataaaaaga cacccggata cgaagggaaa cgcgctgtgt
    12001 gacagacccg ccatgacctt ggatttcaga gaatacagtc tttgtaaaac tgagcgagcg
    12061 aacaggttca tgccataggt ggggcaaggc tggctccacc ggaacaggcg cagaacccgg
    12121 atacttgcgt ttagtcacag tccgagcgtt gaagcggcct tgcaagccgt gagccgtcgc
    12181 aggcaatgag aacgccttgc catcagaatg gggagagttg aaatactatg gggcatgaag
    12241 cctatgtccg tccggtgtgt ctgaaatgaa gtgaaaacct atggggagcc cccggcaatg
    12301 ctgtctgatg ataggccaac cgatccggcg tggctcccca tcttttcaaa aaaggagcac
    12361 tttatgggaa atgcgtttgg aatgatcccc ggcttggaac cggacgaatg gagcaatgac
    12421 gcttgccgtg gttatgtcat tatggcaatg gaggactgcg gattttcaaa gaaggatata
    12481 cggcgtgttg tggggcagct ttatgaagta tttgacctca acagcgtgga ggacgccaaa
    12541 gaaaagtacc attccagtcc ttactgacct cgggccacat cgacccgaag tggagaaagc
    12601 tcaaagggcg gcacctgcgg ggtgccgtc
//
LOCUS       DBGJFF010000345         9554 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.guided.142, whole genome shotgun sequence.
ACCESSION   DBGJFF010000345 DBGJFF010000000
VERSION     DBGJFF010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9554
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.guided.142"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            602..1293
                     /locus_tag="ACOIAF_004035"
                     /pseudo
     CDS             602..1293
                     /locus_tag="ACOIAF_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728879.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            1290..2225
                     /locus_tag="ACOIAF_004036"
     CDS             1290..2225
                     /locus_tag="ACOIAF_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HGZ2670958.1"
                     /translation="MKMDEQTIILFLLLLAIGLLFLSIFAFSKLGHIYKRLKDIEEIL
                     ADVQIGDENRKILIKPCDVMAPLVYQLNEIVYDYENKLLSLKKSDKASKQLMTSLSHD
                     VRTPLTTLIGYLNAVHSGIVIEQEREEYLEIARRRAYDLKDYIDVLFDWFRLNSDEFT
                     LSIESVEIAELSRNILKDWIPIFHEKKLNFEIDIPENRLMVNLDPDGYSRVVNNLVQN
                     VLAHSKARQIKITMSEDSRMVLLRVEDDGVGIARENLPHIFERLYKCDKGRSEKGSGL
                     GLSIVSQMVERMGGQISVESEIGKYTVFTVSLPLI"
     gene            2373..3296
                     /locus_tag="ACOIAF_004037"
     CDS             2373..3296
                     /locus_tag="ACOIAF_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2670959.1"
                     /translation="MNNLIIETKNLTKQYGAQKSVSNLNIHVQKGRIYGLLGRNGAGK
                     TTTMKMLLGLTKPTTGEIKIFGQDIHNNEKKLLPRIGCLIESPGFYPNLTGTENLKIF
                     ARLRGIPKKNAVESALDVVGLPNNDKKLFSQYSLGMKQRLAIALAIMHDPQLLILDEP
                     INGLDPIGIAEVRTFIRDLCTERGKTILISSHILSEIALLADDIGIIDHGILLEEESL
                     AQLEEKNSKYIHFAVSDAAQAAMLLETQLNLKNFKVADNYNIMLYDTDVSVAEINRAF
                     ILNNIEVSESHLCEDTLEDYFKKVTGGEGIA"
     gene            3280..4050
                     /locus_tag="ACOIAF_004038"
     CDS             3280..4050
                     /locus_tag="ACOIAF_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2670960.1"
                     /translation="MKELLDIISCEFSKLKRLKFILISILGACLFPIPATILIARDNL
                     PFEQLFKLVVNFGYFLLLPIVLSIVASQLFFIERDNDVLKNLATVPVQKGKLALAKLA
                     VLLFISLFYSVAGLGATIIGGFIVGIVEGVAVKLGMSIAFGIMFFVTVLPVVVLIVYF
                     NRSYIFSIILSFVYAIFNFSISLNIINFEPNNPLLSVLPAPVIMRWWMAFWGDPTGEY
                     TVLRQPYLLSTPACVGILCLITIIAVLLICMIYKKQEN"
     gene            4060..4842
                     /locus_tag="ACOIAF_004039"
     CDS             4060..4842
                     /locus_tag="ACOIAF_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2670961.1"
                     /translation="MLDIITTEFQKIKRYNILWIGVVAVLFSALLAVFQVVSSHGSDP
                     LTYESFANGVIWNNFSLSFPFGITLIGGYLINREYTDQTLKNILTVPISLRKLLVGKL
                     IAVGGITVLLSLFSFLCTFILGLLFCNINISVILVVKSLVQILSVNLYCYVAVLPIIA
                     YFGRKQNAFLTGVGIAFVYGFCGVFVAGRNLTDFYPITAGLGLVGYNNGAGTVYQPLI
                     GAMTLIVILILTTILLVFTPNYDKVMTISKKKGIKHKKYSHK"
     gene            5219..5755
                     /gene="rbr"
                     /locus_tag="ACOIAF_004040"
     CDS             5219..5755
                     /gene="rbr"
                     /locus_tag="ACOIAF_004040"
                     /EC_number="1.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016692 - NADH peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin"
                     /protein_id="HGZ2670962.1"
                     /translation="MNLKGTKTEKNLMAGFAGESEARNKYTYYASKARKEGYNQIAAI
                     FEETANNEKEHAKLWFKLLHDGMPSTEENLKDAAAGENYEWTDMYAKFAKEAREEGFD
                     KIAYLFEAVGKIEKEHEERYLKLLENLNEGKIFKRDEEVVWQCQNCGHVYVGTEAPEK
                     CPVCDHPKAYFNIKAENY"
     gene            5787..6176
                     /locus_tag="ACOIAF_004041"
     CDS             5787..6176
                     /locus_tag="ACOIAF_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HGZ2670963.1"
                     /translation="MKFSKQRELILNEILNNPVHPTADYLYENLKKDNPNLSLGTVYR
                     NLAQLTEHGFIRKVSIPGYPDRFDGRIDNHYHIICEVCGEVYDLESEVLNNLQELISE
                     ETDIKITSYNISFKGICNNCKRCSQVG"
     gene            6213..6599
                     /locus_tag="ACOIAF_004042"
     CDS             6213..6599
                     /locus_tag="ACOIAF_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin family protein"
                     /protein_id="HGZ2670964.1"
                     /translation="MCSEQKFFICETCGNLVGMIQSGGVPIFCCGKPMKELVPNTTDA
                     AVEKHVPVIEVDGNNVTVKVSSTTHPMTKEHHIAWVYLMTEQGGQRKCLAVDGEPVVK
                     FALNDDDKVISAYAYCNLHGLWKAEL"
     gene            6726..8168
                     /locus_tag="ACOIAF_004043"
     CDS             6726..8168
                     /locus_tag="ACOIAF_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HGZ2670965.1"
                     /translation="MSIYKCSVCGYIYDESKNDKTWDELSEDWECPVCTKGRSYFGKI
                     STVYYEEDEKIAEDIVEDESKLNTEKEGDLNYLSTYLRRDDEVEKHMDIIHEMAVTGK
                     SIIEPMRTKLPVISWDDILIMGAQLNPLPLNEHDEVNTTTIIGKKAKKPMIIENPVYI
                     SHMSFGALSKELKIALAKGAAQNKTAMCSGEGGILPEEKEASYKYIFEYVPNKYSVTE
                     ENLKNSDAIEIKIGQGTKPGMGGHLPGEKVTEEIAKVRNKPVGQDVISPSCFEEIQSK
                     EDLKKLVDELREVSEGRPIGVKISAGHIEKDMEFIAYAKPDFVTIDGRGGATGASPKL
                     LKDATSIPTIFALYRARKYIDTHGLDIDLVITGGLRISTDFAKAIAMGADAVAIASSA
                     LMAAACQQYRICGSGKCPVGVATQDEELRKRLHIENSANRVANFLNVSLEELKTFARI
                     SGHKDIHDLSVDDLYTVNSEISNYTNIQHV"
     gene            8465..9280
                     /locus_tag="ACOIAF_004044"
     CDS             8465..9280
                     /locus_tag="ACOIAF_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HGZ2670966.1"
                     /translation="MERLKIQVLVDNNTYIDRYFVGEPAVSYYIEIDGNRILFDSGYS
                     DVFISNAEKLNIDLGNLTHVVFSHGHNDHTRGIQFLENRYDLSTVELVSHPNCFIPRK
                     HGEKSIGAPFSAEEIKNIFMYNPKDKPFNLSKNCVFLGEIPSINDFEKRAKIGKCKIG
                     DLWEDDYVLDDSAIVCKTDKGIFIVTGCSHSGICNIVEYAKKVCGDDRVIGILGGFHL
                     FELNNRLDSTIQYLDKEEIRMLYPCHCVSFKVKAKMNEILNINEVGVGLTISI"
BASE COUNT         3349 a         1232 c         1804 g         3169 t
ORIGIN      
        1 gaataattgc attacttaag ggggttgtct taaaattgat ttttttaatt aaatttgaag
       61 ctcaactcct tttttaaacc tttatatcat tacaacttta atgtttatac gcctgtactt
      121 cagatgtata tctacttttt acatttctca acatgcaaac ctaccatttt ttgcagtatg
      181 ttaattgaaa taaaccattg agttaaccct aaatttattc tggacttata ggaatagttc
      241 caaagatact ttaaaatatt ttctggaata attgcttatt tcacatatgt tttattgtga
      301 tgttttgatt taataaattc ttcaactctg tatatagtaa atgtattatc atgtttgttt
      361 tattcaagca cacttataat acgcttgatt ttttcacaat gatgaaatat actaatgggt
      421 gttataggta taagaatcgc attacacttg aagaagtaag tgataaatga gatataaatc
      481 ctcatatgat aaactactat ttcattgagg aacatattct aagtgatgtc aagatactac
      541 aatatgacta aattcctagt gataaaaaag tctacgaatg agtgtagaaa ggggaactaa
      601 gatgaataaa gttttaatta tagatgatga taaagatctt tgtattttgt taaaaaagag
      661 tatttcaatt gaacagattg aagcaaacta ttgccattct gggagagatg gactggttga
      721 actttcaaaa caagattatc agttagtagt tctagacgtg atgatgccag gtatggatgg
      781 ttttgaaact atggaacaaa tacgaaaata tagcagtatt cccatcttaa tgctgacatc
      841 aaaaagcgat aattacagca aagtgcatgg attgcgagct ggtgcagatg actacctaac
      901 caaaccattt gaaattacag aatttactgc tcgtgtactg tctttaatcc gacgttacac
      961 ccgttttaat ccaaaaaatg aggaaccaca gcaactttct ttcaaaggtt tgttgattga
     1021 ccttgaccag catacagtga ctaccaatgg cgaacctatg ttgcttccac ccaaggaatt
     1081 tggtgtactt ctttactgtg caaggcatca gggtaagatt ttgacaaaac agcagattta
     1141 tgaagccgtg tggggtgagc catatgtata tgatgacagc aatatcatgg ctattatcag
     1201 tcgcctaaga aaaaaattga actagaccca tcatctccca tctatttgca aacaataaaa
     1261 ggaattgggt atcaattcaa cacggaggta tgaaaatgga tgaacaaacg ataattcttt
     1321 tcttactctt gctggcgatt ggtctacttt ttctttccat atttgctttt tccaagcttg
     1381 ggcatattta caagcgccta aaagatatag aggagatact cgctgatgta caaattggag
     1441 acgaaaatcg taaaatacta attaagccat gtgatgttat ggctccattg gtttatcagc
     1501 ttaatgagat tgtctacgat tatgaaaata agttactttc tctgaaaaag tcggataaag
     1561 ctagcaaaca acttatgaca agtttatctc atgatgttcg tacaccatta acaactctga
     1621 ttggttatct gaatgctgta cacagtggta tagttatcga acaagagcgg gaggaatacc
     1681 ttgaaatcgc taggcgtaga gcctacgacc tcaaggatta cattgatgtt ctttttgact
     1741 ggtttcggtt gaactctgat gaatttacgc tgtcaataga atctgtggaa attgcagagt
     1801 tgtcacgcaa tattttgaaa gactggattc ctatattcca tgaaaagaaa ctgaattttg
     1861 aaattgatat tccagaaaat cgactaatgg taaacctcga cccagatgga tattcccgtg
     1921 tggtcaataa tctggttcaa aatgttcttg ctcacagtaa ggctcggcaa atcaagatta
     1981 ccatgagtga ggattccaga atggtattac tccgtgttga ggatgatggt gttgggattg
     2041 ccagagaaaa tttaccacat atttttgagc gactgtataa gtgtgataaa ggacgttcag
     2101 aaaaaggaag tggtcttggt ctatctattg tcagtcaaat ggtggagcga atgggtggtc
     2161 agatttccgt agaaagtgag attggaaagt ataccgtatt tacagtgagc ttaccattaa
     2221 tataaaattg tttttccccc atgtgacatg tatacacagg gggatttttt gtaagttata
     2281 aagttgcaag gttaatacaa ggttgttgca aggttaatgt aagattgagg tggtatgata
     2341 actttaataa acaaaggagg cttttattat ttatgaataa tttaattatt gaaacaaaaa
     2401 atctcactaa gcaatatggt gctcaaaaga gcgtttctaa tttgaatatt cacgttcaga
     2461 aaggtcgcat ttacggattg ctaggaagaa acggtgcggg taagacgaca actatgaaaa
     2521 tgttattggg tctaacaaaa ccaactactg gagaaattaa aatatttggt caggacattc
     2581 acaataacga gaagaagctt cttcctcgaa ttggatgttt gattgaatca ccagggtttt
     2641 atcctaatct gacaggaaca gaaaatctca aaatctttgc aaggcttaga ggaataccaa
     2701 aaaaaaatgc agtagaatca gcactggatg tagtcggttt gccaaacaat gacaaaaagt
     2761 tattttctca atactctctt ggtatgaaac agaggttggc gattgctctt gccattatgc
     2821 atgacccaca actgttgatt ttggacgagc ctatcaatgg acttgatcct attggtattg
     2881 cagaagtaag aacgtttatt cgtgatctct gcacagaacg aggtaaaact attttgattt
     2941 ccagtcacat tctttctgaa attgccttgt tggcagatga cattggcatt attgaccatg
     3001 gtattttgtt ggaggaagaa agtcttgccc aattagaaga aaaaaacagt aaatacatac
     3061 attttgctgt atctgatgca gcacaggcag caatgcttct ggaaacccaa cttaacctga
     3121 aaaatttcaa agtagcagat aattacaata ttatgctata tgatacagat gtttctgttg
     3181 cagaaataaa tcgtgctttc attctaaaca acattgaggt atcagaatct catttatgcg
     3241 aagatactct tgaagattac ttcaagaaag taacaggggg tgaaggaatt gcttgatata
     3301 atttcatgtg aattttcaaa actcaagcgg ttaaaattta tactgatttc tattttaggt
     3361 gcttgcttat ttccaattcc cgcaacaatt ttgattgcaa gggacaactt gccctttgag
     3421 caattgttca aattagttgt caattttgga tattttttgc tattaccaat tgtattaagt
     3481 attgttgcta gccaactatt ttttatagaa agagataatg atgtattaaa aaatcttgca
     3541 acagtgccag tgcaaaaagg aaaactggct ctggcaaaat tggctgtgtt gctgtttata
     3601 tcattgttct actccgttgc agggttagga gctactatca ttggaggttt cattgttgga
     3661 atagtagagg gggttgctgt aaaattagga atgagcattg cttttggaat tatgtttttt
     3721 gttactgtac ttcctgttgt ggtactcatt gtatatttca atcgcagcta tattttttct
     3781 attatacttt cttttgtata tgctattttc aattttagta tatccttaaa tattatcaat
     3841 tttgaaccaa ataacccact attaagcgta ttacctgctc ctgttataat gcgttggtgg
     3901 atggcatttt ggggagatcc cactggcgaa tacacggtac ttagacagcc atatttgctc
     3961 tcaactcctg cttgtgtagg tattttatgt ttgataacca ttatagcagt cctgctgatt
     4021 tgtatgatat acaaaaagca ggagaattag gaggtatcta tgcttgatat aattactaca
     4081 gaatttcaaa aaataaaaag atataatatt ctatggattg gtgttgttgc tgttctgttt
     4141 tcagcactat tggcagtttt tcaggttgta agttcacatg gcagtgaccc tctaacgtat
     4201 gagagctttg caaacggagt tatctggaat aatttcagtc ttagttttcc atttgggatt
     4261 acacttattg gaggatatct tattaaccgt gaatatacag accaaacctt aaaaaacatc
     4321 ctaactgttc ctatttcatt acgcaaattg ctagtgggaa aattaattgc ggttggagga
     4381 attactgtat tgttaagtct tttcagcttc ttatgcacat ttattttggg gttgttattt
     4441 tgcaatatca atatatctgt tatactcgtt gtaaaatcct tagttcagat tttgtctgtt
     4501 aatctgtact gttatgttgc tgttttgccg attattgctt attttggtcg taaacaaaat
     4561 gcatttctga cgggtgtagg gattgcattt gtatacggtt tttgtggtgt gtttgttgct
     4621 ggaagaaatc tgacagactt ttatccaatc acagcaggtc ttggtctagt tggatacaac
     4681 aatggtgctg gtactgttta tcagccactt atcggtgcta tgactttgat tgtaattcta
     4741 atcttgacaa ctattctact tgtatttaca cctaattatg ataaagttat gaccatatct
     4801 aagaaaaaag ggataaagca taaaaagtat tctcataaat aacataaata ttgtttctat
     4861 tatttaagtt gctagacagt gaaaagaaaa aatctgtcgt acagtggcta tatgctcatg
     4921 tattcaagaa gctacagttt tgatttttca taactgtagc ttctttttgt atttattaaa
     4981 tcacgactat cataatactt gcaataggta tagcgacaag agagagtact gtcatatgtg
     5041 ttttatatat tacattaagt atattctaaa atatatgttt taaataatat atttgaaaat
     5101 aggaatattt aaataaaaaa aataatatta tatatagttg acaaaaattg gcaaatgaga
     5161 tactatataa atatagaaat agtaatcatt actattatta aaaagggagg aattaattat
     5221 gaacttaaaa ggaactaaaa cagaaaagaa tttaatggca ggatttgcag gagagtcaga
     5281 agcaagaaat aaatatacat actacgcttc aaaagcaaga aaagaaggtt ataatcaaat
     5341 agctgctata tttgaagaaa ctgctaacaa tgaaaaggaa catgctaaat tatggtttaa
     5401 acttttacat gatggtatgc cttcaacaga ggaaaattta aaggatgcag cagcaggtga
     5461 aaactatgaa tggactgata tgtatgcaaa atttgctaaa gaagctagag aagaaggatt
     5521 tgacaagata gcttatttat ttgaagctgt tggaaaaata gagaaagagc atgaagaaag
     5581 atacttaaag cttttagaaa acttaaatga aggaaaaata tttaaaagag atgaagaagt
     5641 tgtttggcaa tgtcaaaact gtggtcatgt gtatgtaggt actgaagcac cagaaaaatg
     5701 cccagtatgt gaccatccaa aagcatattt caatataaaa gctgaaaatt attaaaaatt
     5761 atcattatta aaaaggaatg ttaactatga aattttctaa acaacgagaa ctgattttaa
     5821 atgaaatatt aaataatccg gttcatccta ctgcggatta cctatatgaa aacttaaaaa
     5881 aagataatcc aaatttaagc ttaggaactg tgtatagaaa tttagctcag ctaacggaac
     5941 atggctttat aagaaaggtt agtattccag ggtatccaga tagatttgat ggtcgaatag
     6001 ataatcacta tcatatcata tgtgaagtat gtggagaggt atacgattta gaatccgaag
     6061 ttcttaataa tttacaagaa ttaatatctg aggagacaga cataaaaata acatcttata
     6121 acataagttt taaagggatt tgtaataatt gtaaaaggtg tagccaagta ggttagtttt
     6181 caaattctaa aaataattaa tgaggtgagt ttatgtgtag tgaacaaaaa ttttttatat
     6241 gtgagacttg tggaaacctt gtaggaatga tacaaagtgg tggtgtacct attttctgtt
     6301 gtggaaaacc tatgaaggaa cttgttccta atacaacaga tgcagcagtt gaaaaacatg
     6361 tacctgtaat tgaagtggat ggaaataatg ttactgttaa agtaagtagc acaactcatc
     6421 caatgactaa agaacatcat atagcatggg tgtatcttat gactgaacaa ggtggtcaac
     6481 gcaaatgtct agcagtagat ggagaacctg tagttaaatt tgctttgaat gatgatgata
     6541 aagtgatttc tgcctatgct tactgtaatt tacatggatt atggaaggct gaactgtaat
     6601 atttaaataa attgagtcaa aaaggaggct ataaatgtat tttcatacaa atagcctcct
     6661 ataaatttat ataaattcaa gttttaattt tgtttaatgt caatataata taaggagaaa
     6721 ataatatgtc aatttataaa tgtagtgttt gtggatatat ttatgatgaa tctaagaatg
     6781 ataaaacatg ggatgaatta agtgaagatt gggaatgccc tgtatgtaca aaaggtcgta
     6841 gttattttgg aaagataagt actgtttact atgaagaaga tgaaaagata gcagaagaca
     6901 tagttgaaga tgaatctaaa ttaaatactg aaaaagaggg agatttaaat tacttaagta
     6961 catatttaag aagagatgat gaagttgaga aacatatgga tataattcac gaaatggctg
     7021 ttacaggaaa atctataata gaacctatga gaactaaact accagtaatt tcttgggatg
     7081 atattttaat tatgggtgct caattaaatc cattaccttt aaatgagcat gatgaagtaa
     7141 atacaacaac aataatagga aaaaaagcaa agaaacctat gattatagaa aatccagttt
     7201 atatttccca catgtcattt ggagctttgt ctaaagaact aaaaatagct ttagcaaagg
     7261 gggcagccca aaataagaca gctatgtgta gtggtgaagg aggtatatta ccagaagaga
     7321 aggaagcttc atacaagtat atttttgagt atgtaccaaa taaatatagt gtaactgaag
     7381 aaaatcttaa aaattctgat gctatagaga taaaaattgg tcaaggaaca aaacctggta
     7441 tgggaggtca tcttcctggc gaaaaagtaa ctgaagaaat agcaaaagta agaaataagc
     7501 ctgtaggtca agatgtaata agtccatctt gttttgagga aatacaatcc aaagaagatt
     7561 taaagaaact tgtagatgaa cttagagaag tttcagaagg gcgtccaata ggtgttaaaa
     7621 tttcagcagg tcatattgaa aaagatatgg aatttatagc ttatgctaaa cctgattttg
     7681 taactattga tggtcgtggt ggagctactg gagcaagtcc aaagttgctt aaagatgcaa
     7741 cttcaattcc aacaatattt gctctttatc gtgctagaaa atacatagat acacatggat
     7801 tagatataga tttggttata acaggtgggc ttcgtatatc tactgatttt gctaaggcaa
     7861 ttgctatggg ggctgatgca gttgctattg caagttctgc tcttatggca gcggcgtgtc
     7921 aacaatatcg tatatgtggt tctggtaagt gtcctgtagg agtagctact caggatgagg
     7981 aattgagaaa aagattacat atagaaaata gtgctaatag agtggcaaac tttttaaatg
     8041 taagtttaga agaacttaag acttttgcta gaatttctgg tcataaagat atacatgatt
     8101 taagtgttga tgatttatat actgttaact ctgaaatatc aaattataca aacatacagc
     8161 atgtatagat tgaagtttga aactattaga ttttagatag ctataaaagt aaatgataaa
     8221 tatattttat aaaagagagt gtcttaaaat gaacttttta gttcataagt aactcttttt
     8281 tgtatgaagc caaatatatt tatcatttta gcaatgaaaa agaatcttat ctctttattt
     8341 tgagatagac tccttctatt tatgaaaatt aaagatatta aattgtaaaa tattctgtga
     8401 tgtcgtacta ttaaactaaa gattctgtac tttaaagtaa ataaaaattt aaggagtgat
     8461 tattatggaa agattgaaga tacaagtttt agtcgacaat aatacttata ttgatagata
     8521 ctttgttgga gaaccagcag taagctatta tattgagatt gatggaaata ggattttgtt
     8581 tgactcaggg tattcagatg tattcatatc aaacgctgaa aaattaaata tagatttagg
     8641 aaatttaact catgttgttt tttctcatgg gcataatgac catacaagag gtatccagtt
     8701 tttagaaaat aggtatgatt tatctacagt tgaacttgtt tcacatccta actgttttat
     8761 acctagaaaa catggagaga aaagcatagg agctccattt tctgctgagg aaattaaaaa
     8821 tatttttatg tataatccta aggataagcc atttaatcta agcaagaatt gtgtgttttt
     8881 aggtgaaata ccttctataa atgattttga aaaaagagct aaaattggta aatgtaaaat
     8941 aggagatttg tgggaagatg attatgtttt agatgattct gctatagttt gtaaaactga
     9001 taaaggtatt tttattgtta caggatgttc tcatagtgga atttgtaata tagtagagta
     9061 tgctaaaaag gtatgtggag atgatagagt tattggtata ctaggtggtt ttcatctttt
     9121 tgaattgaat aataggttag atagtacaat acaatatttg gataaagaag aaataagaat
     9181 gttgtaccct tgtcactgtg tttcttttaa ggtaaaagcg aagatgaatg aaatattgaa
     9241 tataaatgaa gttggagtag ggctgaccat aagtatatag tatttgaaag tatataagat
     9301 ttaagttgaa ggttaaaaag atattttcta tagaaaatat cttttttgtt acgtttattt
     9361 taagagaaat caatatactt ttatatgaaa tattgtaaaa aaatttattt ttaatgagaa
     9421 tatattttca ttaataaaca attttattat gtattaataa tgttagaata aatataaata
     9481 aatcaaagtt acttacatta cagtatattt tttcacaaaa tatattgatt tattctttta
     9541 aagtttgata attc
//
LOCUS       DBGJFF010000346         7544 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.guided.165, whole genome shotgun sequence.
ACCESSION   DBGJFF010000346 DBGJFF010000000
VERSION     DBGJFF010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7544
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.guided.165"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            331..1032
                     /locus_tag="ACOIAF_004045"
     CDS             331..1032
                     /locus_tag="ACOIAF_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-G-Cd"
                     /protein_id="HGZ2670967.1"
                     /translation="MNENILIVDDEKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KNLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKTQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            1082..2164
                     /gene="vanS"
                     /locus_tag="ACOIAF_004046"
     CDS             1082..2164
                     /gene="vanS"
                     /locus_tag="ACOIAF_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS"
                     /protein_id="HGZ2670968.1"
                     /translation="MRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKA
                     ALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEV
                     ALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
                     LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLE
                     QLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEIT
                     AVQEDDYIKLKFMNHSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVEL
                     HNGTITAHSENNIITFKVIIPIVGNL"
     gene            2425..3525
                     /gene="vanG"
                     /locus_tag="ACOIAF_004047"
     CDS             2425..3525
                     /gene="vanG"
                     /locus_tag="ACOIAF_004047"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HGZ2670969.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNNDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            3522..4328
                     /locus_tag="ACOIAF_004048"
     CDS             3522..4328
                     /locus_tag="ACOIAF_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HGZ2670970.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            4346..6484
                     /gene="vanT"
                     /locus_tag="ACOIAF_004049"
     CDS             4346..6484
                     /gene="vanT"
                     /locus_tag="ACOIAF_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HGZ2670971.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKKLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKPLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVKMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSSPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINGCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLSE
                     LSASEVANNSCSISNELLSRVGRRLNVIKK"
     gene            6798..7418
                     /locus_tag="ACOIAF_004050"
     CDS             6798..7418
                     /locus_tag="ACOIAF_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HGZ2670972.1"
                     /translation="MQYIQELLDLTQNYWVLATIIGLLSAFIESFIPALPLVAIVTAN
                     AAIQGLFIGCLLSWIGSGLGTTSLFLLISRFNDSKLFNKLRNSKTEKAISWMDKQGFK
                     LLFIAYACPFMPGCLVTIASAFGKKDLKDFVPAMLAGKFVMFIVISYVASDIEGFITS
                     PLKIASFILLVFLSWKIGNRVNKNLENHNYDFHHKKHHNDKDDKMI"
BASE COUNT         2763 a          838 c         1299 g         2644 t
ORIGIN      
        1 aaattttagt aatgtctgtg gttacataca taggtttaat tattttttat atggattgtc
       61 tctggtataa taaattgaat tgtgtttgac tgatagatat ttttattgaa agttaatacc
      121 tgttatgcga taataaggac gtgttagggg agtttttata ataacaatta atctcagttt
      181 ttgtgttcca atggtagcat caacacattc tgatgtagta gttcttttct tataaaaatt
      241 agttacaaat atgcttgtaa ttatttgata tgtaaattta ccaatattat aaatgatgac
      301 ctcgataata aggagagtga aaaataatct atgaacgaaa atattttgat tgtggatgat
      361 gaaaaagaaa tagcagattt aattgaagta tatttaaaaa atgaaggttt tacagtatac
      421 aagttttata caggtaagga agctctagaa tgtattgaat ctaaaaattt ggatatggct
      481 attttagata ttatgcttcc tgatattgat ggatttcata tttgccagaa aattcgagaa
      541 aaatactatt atcctatcat tatgttaaca gctaaaattg aagatataga caagataatg
      601 ggtttaacca taggtgctga tgattatata acaaagccat ttaacccttt ggaagttgtt
      661 gcaagagtaa agacacaatt aagaagatat atgcgatata ataattctta tgaacaacag
      721 tcgataattg ttaatgagta tgatattaaa ggattgatta tcaataaaga aacacataaa
      781 tgtagtttat ttgggaaaga agtagcatta acaccaattg aattttctat tctttggtat
      841 ttatgtgaac atcaaggaaa agtggttcct tctgaagaat tgtttgaagc tgtatgggga
      901 gaaaaatatc ttgataataa taacacagtt atggctcata tagggcgatt acgtgaaaaa
      961 attcatgaac cttctaaaaa cccgaaattt ataaaaactg tatggggagt aggttataca
     1021 attgaaaagt aaaaatatta aagtcaatcc tgactttact caagtgagaa ataaaatttt
     1081 tatgagaaca gctttaatgg catttatagc cattattttc atatggattt tatattcttt
     1141 tatattgtat ggtaagtttt ctatctggat agtatcattc tttgaaaatg cttttcgtat
     1201 ggaatataaa gctgcattga atttatatca gcagattttc cgtaatagca tggagctctt
     1261 catgatacta gctatggtga ttgtgttttt tataatattc cgtgtttatt taaattggtt
     1321 tacaaaatat tttagtgaaa taaatcaagg tattgactct cttatcaaag aagatgttgg
     1381 agaagtggct ttatctccag agttattggc aatagaaaag aaaataaact caattaaaca
     1441 tatacttgaa caacgtaaat ttgagacaca aatggcagaa caacgtaaga atgaacttat
     1501 tgtttatctt gcacatgatt taaaaacacc acttacatca gttatagggt atctgacact
     1561 acttcgagat gaaagtcaga tttctgaaga actgcgaaaa aaatatttat ctatttcgtt
     1621 ggataaggca gagcgtcttg aagatttaat taatgaattc tttgaaatta caaggtttaa
     1681 tttatcaaat atatcacttg aatacagtac agtaagctta actcgtatgc ttgaacaact
     1741 aacatatgag tttaaaccta ctttgatgag taagaattta aaatatacac tgaatgttac
     1801 accaaatatg atgataaggt gtgatgttaa taaaatgcaa cgtgtatttg ataacttatt
     1861 acgaaatgct gtttattata gttttgaaga tactactatt gaaataacag ctgtacagga
     1921 ggatgattat ataaagctta aatttatgaa tcatagcaat acgattccag aggaaaaact
     1981 tgagcgtatt tttgaacaat tttacaggtt ggatacatct cgtaattcaa acagtggtag
     2041 agctgggttg ggacttgcta tagctaaaga aatagtggag cttcataacg ggacaataac
     2101 agctcatagc gagaacaata taattacctt taaggttatt attccgattg taggaaattt
     2161 gtaagaattt aagtataaaa aaataggaaa ttctttagga taaataaata aagagcactc
     2221 ttgttattga tataattact gtatcaaaac aagagtgctt ttattttgat acagtaatta
     2281 tatcaataga aaaatgtgaa aatatattat gtggaacaga tgcaattgta agtgaaggtt
     2341 gattggattt gaaatagaaa aagtatttta acatgtttct gtagattgta taaaaataaa
     2401 taaatcaaca aaggagaatc aaagatgaat aataaaaaaa ttgcagttat atttggtgga
     2461 aactcaacag aatatgatgt atcattacaa tcagcttttg cagtacttga gaatttgaat
     2521 acggaaaaat atgatattat accaattgga attacaaggg agggtaattg gtatcattat
     2581 attggagatt ataataatat ccagaataac acttggtttg aaagggatac tgatataatt
     2641 ccagttattg tttcgcagaa ccgtgtcaaa aatggtatta ttgaattttt aattcctgat
     2701 gctgtttctc agaatcatat tccaaatagt gctattgaat catgtgaaaa tgatattaaa
     2761 tacactaaac ttgattttgt gtttcctgta ctgcatggaa aaaatggaga agatggaaca
     2821 gtgcaggggt tatttgaatt ggcaggtata caggttattg gatgtggtac tctttcttca
     2881 gcattatgta tggacaaaga caaatcacat aaacttgttc attcagaagg tattaaagta
     2941 ccaaaatcaa tattactaaa atactcagat aaatttaatg tggctgaact gtcaaaagac
     3001 ttaacttatc cattatttat aaaacctgtg cgtgcaggtt catcatttgg tattagtaaa
     3061 atttataaaa aagaagagtt acaagtagca gtagacttgg cttttgaaca tgataatgaa
     3121 gtaataattg aggaaaatat tgatggtttt gaggtagggt gtgcagtttt aggaaacaat
     3181 gacttacttg ttggaagagt agatgaaata gaattgtcaa aaggattttt tgattataca
     3241 gaaaaatata cattgaaaag ttcaaaaatt catatgccag ctagaattga tttggattgt
     3301 gagaaaagaa tacaagagac agcaaaaatt atttatagaa ctcttggatg ttctggattt
     3361 gctagagtag atatgttttt aactccatct ggtgaaatta tttttaatga agtcaataca
     3421 attcctggct ttacttctca tagtcgttat ccaaatatga tgaaaggtat tggactgtct
     3481 tttgaagaaa tattggacaa gataattgag gtgtacataa aatgatgaat aatattttat
     3541 tagataaaaa taaagaagat aaaatgacaa atactatctt attgactaaa gataaagttt
     3601 atgatgggaa tctaattcta gtaaatgctt ttttaccagt taaaacatct gaagatatag
     3661 atttaattcc tgtagataca aggtttccaa gtattcttat gaaacgtgaa gctacaaaca
     3721 ttttacaaaa tattttaaaa agtatatgtg gaataaatga aatagtacct gtaagtggat
     3781 atcgtactgc agaggaacaa caagatatat attcaagttc tctgcgtgac aatggaaaag
     3841 actttactaa aaagtttgta gcacttccaa atcacagtga acatcagaca gggcttgcta
     3901 ttgatttagg gttaaagaaa gaagttatag attttatctg ccctgaattt ccttataatg
     3961 gtatttgtaa tgagtttcgt aaggcagcac tacattatgg ttttatagag cgttatcagt
     4021 gtaaaaaaga agaaataaca ggaatatctc aagaaccatg gcattttcgt tatgtgggat
     4081 atccgcattc tgagattatc aataaaatga atctcgctct tgaagaatat atagaatata
     4141 ttaagagatt ttcgtataca aattgtttaa aaatagaaga taaagaaaaa attatagaaa
     4201 tcttttatgt gcctattcat actaaagaag aagtgagaat ctctatacct gaacaatgtg
     4261 tatatcaaat atcgggtaac aataaggatg gttttattgt gacattttgg agaaatagaa
     4321 aggactaaga aatggaggaa caagaatgat taggaataaa tcttatgcag ggcttgatta
     4381 ttttaggatt atagctgcat tacttataat tgcaattcat acatctccat tattatcata
     4441 tagtaaaatg gctgactttg tgttaactag tataattgct cgtattgctg tgccattctt
     4501 ttttatgcta tcaggctttt ttctaatttc aacatataat tataattgta ataaactgaa
     4561 atcttttcta aaaaatgtat tatttattta cgggatagct attgtaattt atattccctt
     4621 aaatatttac actggatatt tttccatgaa taatttagtg cccaatatta ttaaggacat
     4681 tgtatttgat gggactttat atcatttatg gtatcttcct gcatcaatta ttggtgcaag
     4741 tattgcatac tttcttgtaa aaaaactagg tttaaagtac tcactagtaa tcactattgt
     4801 tatttatgta attggtatgt ttggtgatag ttattatggt tttttagaaa aaataccact
     4861 attaaagaat ttatatgggt atatttttga agtatcagat tatacacgaa atggtatatt
     4921 ttttgcacct gtatttttca tacttggtgg aataatatct aataaatcta attatatgtc
     4981 aactaaaaat agcttgattg gatttcttat atcattctta cttatgttat gtgagggaat
     5041 gtttttacat aaactagggg tacagcgtca tgatagcatg tatattatgt tattgccatg
     5101 tatgtatttc ttatttacag ccttaacatt tttgaaaggt tctcgagcta ggttattgcg
     5161 aacatcatca ttaattattt atattataca tcctatgatg attgttgtta ttcgtatgtt
     5221 ttctaaaata ttcggaatac aaactgtctt ggttgacaat agccttattc attatatagt
     5281 ggtaagtata gcatctttta tagcttctat aattattata ttgattctta aacctctaaa
     5341 aaaagacttg aaaggagaac ataaggtaaa taccaataga gcttggattg aaattgattt
     5401 aaataatttg gaacataatg taaagatgat acaaaaagct atgccagaag attgtgaatt
     5461 gatggcagtt gtaaaagcta atgcctatgg tcatagtgct tttgaagtag cgacttgtgc
     5521 aaaccaaata ggtgtaaaag catttgcagt agcaacaata gatgagggga ttgatttacg
     5581 tcgatatgga atactaggag aaatattgat tcttggatat actaacccaa taagggcaaa
     5641 ggaacttcat aaatacaatc ttacacagag tgttattgac cttaatcatg ctatctcttt
     5701 aaacaaacag aaatacaaca taaaatcaca cattaaaatt gatacaggta tgcacagact
     5761 gggttttgat gttaatgatg tgaggtcaat tttaaaggca tttaaattaa aatacttaga
     5821 tatatgtggc atatttacac acttgtgtgt ttcagatagt ctgaaagatg aagatgtaga
     5881 ctttacaaat aaacaaattg aatgttttta taagttaatt gattttctgt taaaaaaagg
     5941 tataaaaatt cctaaagtac atattcaaag tagttatggt ttttttaatt atcccaatct
     6001 taagtgtaat tatgtaagaa ttggaattgc attatacggg atattaagtt ctccaaatga
     6061 tacgactaaa ttgcaacttg atttacgacc tgttttatcc ttaaaatcac aagtaattct
     6121 tattagaaaa atacaaaaag atgatagttt tggatatgga agagtcttta ttgcaagcag
     6181 agatagcgta attgctatat tatctattgg ctatgccgat ggtataccta gaaatttatc
     6241 ctgtggaaaa agttacgtaa taataaatgg ttgtagagca gcaatagtgg gtcgaatttg
     6301 catggaccaa cttgctgttg atattacaga tatacctaat gtagaagttg gtaatacggc
     6361 aataataata ggaagagata atttatctga attatctgct tctgaagttg caaataactc
     6421 atgtagtata tcaaatgaat tattgagcag agtaggaagg aggttaaatg ttataaagaa
     6481 gtaactcaat taaatagtaa tatttgttgt actgtgtaat ttattttact ctgactagac
     6541 atttttagta atattatgtc taatatatat tttttataac ttacatgaca atttaagcta
     6601 taaaaaggtg atgtttcaga tttttatttt gaaacatcac cttttttgtt ataatatata
     6661 ataaaataat tataccttaa ctgtaaactt atatagatta tttacttttt aatattagat
     6721 tcattttttg agattggtat ataataaaca tatagagtat ataataatag ttaaagttaa
     6781 aaaaggggaa aagcaatatg cagtatatac aagaactact tgatttgact caaaattatt
     6841 gggtgttagc cacaataatc ggcttattaa gtgcatttat agaaagcttt ataccagcct
     6901 taccacttgt tgctatagtt acagcaaatg ctgcaataca aggattattt attggatgtt
     6961 tgttatcatg gattggctca ggacttggga caacttcatt attcttatta ataagcagat
     7021 ttaatgatag taagcttttt aataagttga gaaatagtaa aactgaaaaa gcaataagtt
     7081 ggatggataa acagggattt aagttattat ttatagcgta tgcctgtcca tttatgcccg
     7141 gttgtttagt tactatcgca tcagcttttg gtaaaaaaga tttaaaagat tttgtgccag
     7201 caatgttggc aggaaaattt gtaatgttta tagttataag ttacgtggca agtgatatag
     7261 aaggttttat aacaagtcct ttaaagatag catcatttat attattggtg tttttgtcat
     7321 ggaagattgg aaatagagta aataaaaatc ttgaaaatca taattatgat ttccatcata
     7381 aaaaacatca caatgataaa gatgataaaa tgatttagct tgtacgaaat aaaacgtatc
     7441 ttaaaatgat ttagtactaa attattttaa gatacgtttt attttttgta ttaaagtaca
     7501 aaatttttag ttcttttgta aactaaaata tataaaactt attt
//
LOCUS       DBGJFF010000347         5839 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.guided.189, whole genome shotgun sequence.
ACCESSION   DBGJFF010000347 DBGJFF010000000
VERSION     DBGJFF010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5839
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.guided.189"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            <1..572
                     /locus_tag="ACOIAF_004051"
     CDS             <1..572
                     /locus_tag="ACOIAF_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HGZ2670973.1"
                     /translation="KTYKKKETILFAEQENEYVILMIDGIAEAFILNVKGNISTIHLY
                     RSGSFFGEMEQFNDGKKPVEIVAFTDCNVYKLHRDDFLNWLRGDFEATKFLIREISSK
                     LVVNAELIEELSSLSVKERLLRCISLHYYRSELNLLTKKQLASEVNAPIRSINRAINE
                     CVQEHLIAYENKRFSVINQREVLKNLPTT"
     gene            728..1510
                     /locus_tag="ACOIAF_004052"
     CDS             728..1510
                     /locus_tag="ACOIAF_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HGZ2670974.1"
                     /translation="MTKTQQKMIQNVLEFSKRDSRIIGLAISGSYITKSLDEYSDLDF
                     LIVADDKSYEEVIEERLLIVEQFGTLVSAFTGEHVGEPRLIISLYDSPVLHVDFKFTT
                     LYGLLDRVEDSAILYEENNCITEIYSKKEAYFPTPDLQWIEDRFWIWVHYACTKIGRR
                     ELFETIDFLSFLRQSVLAPLIQLLLGKLPRGVRKIELDAPQYIEALEKTVANHEYKSC
                     VNALQATINLYLELREELSQSPIVRKVEAERVALQYFQDLIK"
     gene            1635..2561
                     /locus_tag="ACOIAF_004053"
     CDS             1635..2561
                     /locus_tag="ACOIAF_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2670975.1"
                     /translation="MSDILSRITNKIGDKNIVDKLSALSKSDLNSLLLEVFDRQANTL
                     TATDILKSYQLNRFTIPSGIDPEEIHALESNLLRKAFNMDIKTIMLSPSAPLGSCSVF
                     GSVDQYNVVSALRGTETLADPSNMLAIIIADKLKRKEENNTIPIHMATTARVIRAQNF
                     VGKGLYSHFGLYCMVSSGIDTGSYTCEKMLLEKHLNYYKDILSEIENVRFSIILRKRN
                     GYKDNDGFFDRMVETIKLIFPNIELSIHNDDVDNNYYKGINFKISVKQGNETVEIADG
                     GFVDWTYQMLGNKKERCLISGIGLERFLVAVS"
     gene            2575..3024
                     /locus_tag="ACOIAF_004054"
     CDS             2575..3024
                     /locus_tag="ACOIAF_004054"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGZ2670976.1"
                     /translation="MELIEWNYAYITELVEFANDKEIANNLRDNFPHPYREQNARDFI
                     SFCLATPDVKQLSYAIFYQERAIGSISLTFEEDTSINKNAELGYWLARNYWGKGIATN
                     AIKEICKIAFEKYNLNKIHANVYSYNRGSSKVLEKCGFKIEEYCHNS"
     gene            3438..3791
                     /locus_tag="ACOIAF_004055"
     CDS             3438..3791
                     /locus_tag="ACOIAF_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HGZ2670977.1"
                     /translation="MRNYARDIYDENKRRNDYLVSLESLSLAELSKLSILDDYDSNYI
                     CMVSYDYNIRIEDVLMAQAIGKLTKRKQDIILLSFFLNMTNADIATLMDLAENTIHYH
                     KTNALKELKELMEEH"
     gene            3793..4023
                     /locus_tag="ACOIAF_004056"
     CDS             3793..4023
                     /locus_tag="ACOIAF_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGZ2670978.1"
                     /translation="MKPTKEVLPFTIILLAVHGDVNAINGILKHFEHYIIRLSQKTLF
                     DEYGNPYIHVEEETKRMLGTKLITAILRFNLS"
     gene            4413..4616
                     /locus_tag="ACOIAF_004057"
     CDS             4413..4616
                     /locus_tag="ACOIAF_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HGZ2670979.1"
                     /translation="MNNTDVPIWEKYTLTIEEASKYFRIGEKKLRKLADENLDSGWVI
                     MNSNRIQIKRKQFEKIVDTLDVI"
     gene            4694..5026
                     /locus_tag="ACOIAF_004058"
                     /pseudo
     CDS             4694..5026
                     /locus_tag="ACOIAF_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009271880.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase DNA-binding domain-containing protein"
     gene            5119..5751
                     /locus_tag="ACOIAF_004059"
     CDS             5119..5751
                     /locus_tag="ACOIAF_004059"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HGZ2670980.1"
                     /translation="MTIPSSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVPPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIKHIQSGNIDKLV"
BASE COUNT         2162 a          810 c         1002 g         1865 t
ORIGIN      
        1 aaaaaacgta taaaaagaaa gagactattc tttttgctga gcaagaaaat gaatatgtaa
       61 ttcttatgat tgacggtatt gcagaagcat tcatcttaaa tgtgaaagga aacatctcaa
      121 cgattcatct ttatagaagt ggtagttttt ttggagaaat ggaacaattt aatgatggga
      181 aaaaacccgt tgaaattgtc gctttcacag attgtaatgt atataaactt catagagatg
      241 attttttaaa ctggttaagg ggagattttg aagctacaaa atttctgatt cgagaaatat
      301 ctagtaaatt agttgtaaat gctgaattaa ttgaagaatt atcatcttta agtgttaaag
      361 aacgacttct gaggtgtata tccttacact attatagaag tgaattaaat ctgcttacca
      421 aaaaacagct tgctagcgaa gtaaacgctc caattagaag cattaatcgt gctataaatg
      481 aatgtgtaca agaacatcta attgcttatg aaaacaaaag atttagtgta attaatcagc
      541 gagaggtatt aaagaatctt cctacaacat aagtataatt tcaaaaaagt attgaataat
      601 aattattcaa tgctttttta tttcgggtca agtgacctat ttttttttat ttcttcatta
      661 tataatactc ctatgcataa ttatgttgga ttgatagcta ataaaagaca gtgagaggtg
      721 ttatattatg actaaaacac aacagaaaat gatacaaaac gtattagaat tttcaaaaag
      781 agatagtagg ataataggac ttgcaattag cggttcttat atcacaaaat cgttagatga
      841 atattctgat ttggattttc tgattgtagc agatgataaa tcctatgaag aagttatcga
      901 agagagatta cttattgtgg aacagtttgg aacattagtt tctgctttta ctggcgaaca
      961 tgttggagaa cctcgattga taatttccct ctatgattcc cctgttcttc atgttgattt
     1021 taagtttaca accttatatg gtttactaga tagggttgaa gattcagcta ttctctatga
     1081 agaaaacaac tgcataacag aaatttattc taaaaaagaa gcatacttcc caactccaga
     1141 cttacagtgg attgaagata gattttggat ttgggttcat tacgcttgca ctaagattgg
     1201 acgtagagaa ctatttgaaa caattgattt tttatctttt ttaagacaat cagtacttgc
     1261 cccattaata caacttctct taggtaaatt acctcgtgga gttagaaaaa ttgaattgga
     1321 cgcaccccaa tatattgaag cattagaaaa gacggttgct aaccatgaat ataaaagctg
     1381 tgttaatgca ttgcaggcta ctatcaacct gtatcttgag ttaagagagg aattatcaca
     1441 aagtcctatt gtccgaaagg ttgaagctga aagggttgca ttacagtatt ttcaagattt
     1501 aatcaaataa acccaaagta aaaaaatatt gaatagtaaa tattcagtgt tttttcattt
     1561 ttggtcaaat gacctagatt agttgttgta acaattctat aattattttg aaagtaacag
     1621 tatggaggat aactatgagt gatattttaa gtagaattac aaataaaatt ggggataaga
     1681 atatagttga taagttatca gctctatcaa aatcagattt gaactcactt ctattagaag
     1741 tgtttgatag acaagcaaat actttaactg caactgatat tctaaagtca taccaactaa
     1801 atagatttac tatccctagc ggtatagatc cagaagaaat tcatgctttg gaatcaaatt
     1861 tattgagaaa agcattcaat atggatatca agacgattat gctttctccc tctgccccat
     1921 taggtagctg tagcgttttt ggctccgtag atcaatataa cgtagtaagt gctctacgag
     1981 ggacagaaac acttgcagac ccctcaaata tgttggcaat aattattgct gataaattaa
     2041 aaagaaaaga agaaaataat accataccta tccacatggc tactactgct agggtaataa
     2101 gagcacaaaa ttttgtaggt aaaggtttat attcacattt tggcttatat tgtatggttt
     2161 catctggtat agatacaggg tcttacactt gtgaaaaaat gttgttagaa aagcacttaa
     2221 actattataa agatatatta agtgaaatag aaaatgttcg tttctctatt attctacgca
     2281 aaagaaacgg atacaaagat aatgacggat ttttcgacag aatggttgaa actatcaaac
     2341 ttatttttcc taatatagag ttatcaattc ataatgatga tgttgataat aattattata
     2401 aaggtatcaa tttcaaaata tcggtcaaac aaggaaatga aaccgttgaa atagctgacg
     2461 gtggatttgt tgactggact tatcaaatgc ttggaaataa aaaagaacgc tgtttgataa
     2521 gtggtattgg cttagaaaga tttcttgtag ctgtttcata aaaggagaag aaaaatggaa
     2581 cttatagagt ggaattatgc ttatattacc gagttagttg agtttgcgaa tgataaagaa
     2641 attgcaaaca atcttcgtga caacttcccc cacccttata gggagcaaaa cgctagagat
     2701 tttattagtt tttgtttggc aactccagat gttaaacaac ttagctatgc tattttttat
     2761 caagaaaggg ctattggaag tatcagccta acatttgaag aagatacctc tattaataaa
     2821 aatgctgaat taggttactg gcttgctagg aactattggg gcaaagggat agcaacaaat
     2881 gcaataaaag aaatatgcaa aattgccttt gaaaaataca accttaacaa aattcatgct
     2941 aatgtatatt cctacaatag agggtcaagt aaagtattgg aaaaatgtgg ttttaagatt
     3001 gaagaatact gtcacaattc ataataaaga atagaaacat gattgattgc catgtatata
     3061 gtttaattcg accgcaatag catttattag aattaaccta ctttttaata acagaataag
     3121 tttttcccct ctattaattt cttatgcatc tttgtatttt tatgaaaagc tagccaatgc
     3181 atatttccat acatcaatat ggtggtggct tacaaccaat ataaattaaa tacgttttaa
     3241 agaatacaca tttttgccct ataggcagag tgtgtatttt tttctctttt ttttgaaatt
     3301 tatcgaagat ttaattccct cccaccgtga taggagtaag caaggttatt taactccttg
     3361 cgatggaaag agggtgaaat cttatggttg atgaaaagac aattgaacat caattcgaca
     3421 gtttctgtaa aacagttttg cgtaactatg ctagagatat ttatgacgaa aataaacgca
     3481 gaaatgatta tttggtatct ctcgaatctt tgtcacttgc agaattaagt aaattgagta
     3541 ttttagatga ttatgattct aactacatct gtatggtttc ttacgactac aacatacgaa
     3601 ttgaagatgt attaatggca caagcaatcg gaaaacttac aaaaagaaaa caagatataa
     3661 ttctactttc attctttctc aacatgacaa atgctgacat agcaacattg atggatttag
     3721 cagaaaatac catacattat cacaaaacaa atgcactaaa agagttgaaa gaactcatgg
     3781 aggaacattg aaatgaaacc tactaaagaa gtattacctt ttacaattat tttacttgct
     3841 gtacatggcg atgtaaatgc aataaacgga atacttaaac attttgaaca ctatatcatt
     3901 cgtctttcac agaaaacttt atttgatgaa tacggaaatc cctatattca tgtagaagaa
     3961 gaaacaaagc gaatgctagg aacaaaacta atcactgcta ttttgagatt taatttgtct
     4021 taatctcgcc ctctctccat gtatcaagct atttggtaca tggagaatca ctaattattt
     4081 gtctataaaa gcccacaagc tattgtgatt ttttataggc aaatcaatgc cgactgttct
     4141 ttgacaactg aataaagtag atagatacgt ttgtgataca gatagccaac aaatactata
     4201 cgccatgacc ttaatcaaga gagctactat atagtgattt gtgaataacc tttggaacgg
     4261 ttgttttgcc gtaacctgta ccacagtata atgatacacc tgtatgacac ggttctgccc
     4321 ataagaatgg gattggttga aataccaatg gagcttacca aagccatcta tctacttctg
     4381 aactacgaac atagaaagga gtgatacatt ctatgaacaa tactgatgta cctatttggg
     4441 aaaaatatac cttgacgatt gaagaagcgt caaaatactt tcgtattgga gagaaaaaat
     4501 tacgtaaact tgctgatgaa aaccttgatt ctggctgggt cattatgaat agcaatcgca
     4561 ttcaaataaa gagaaaacaa tttgagaaaa tcgttgatac acttgacgtt atctaatgaa
     4621 attgagcctt atatgttata atattcatat gacgtatcaa gctcaattcg gttattgaga
     4681 ggagcttctt atcatgtcaa ctgaaaaaag acgagataat aaagatagaa ttttaagaac
     4741 tggagagagc cagagaaaag atggaagata cgcatataag tatgtatatt catttggtaa
     4801 accacaattt gtttactctt ggaaacttgt acctactgat aaacccccaa agggaaaacg
     4861 tgatgatata tcattaaggg ataaggaaaa agcaatacaa aatgaccttg atgatagcat
     4921 tgacactatt ggtaagaaaa tgacagtttg tcagttatac gctaaaaaga atgtactaag
     4981 aaagaatatc cgacttaaca ctaaaaaagg acgccattat cttatgaaga ttcttaacaa
     5041 ggagtgattc tgtgaagaga tgtattgtag tagtggaata gtcaaaaaca taatatatat
     5101 taaaggagag atttaaatat gactattcca agttcaaata aggtatatcc aagaagtaat
     5161 gattatcaaa ctgtatatct taaaaatgta ataacaaggg aaaatataga agttggagat
     5221 tacacaatat ataatgactt ttataatgac ccaagagatt ttgagaaaaa caatgtattg
     5281 tatcattacc ctataaacaa tgataaatta ataattggta agttttgctc aatagcatgt
     5341 aatgcaaaat ttctaatgac tagtggaaat cactcaatga aatcaaaatc cacatatact
     5401 tttcccatct tttatgaaga atggaaactc gatatcaacc atataacgga cgcttgggac
     5461 aataagggta acattataat tggaaatgat gtgtggatag gttatgattc tataattatg
     5521 tcaggtgtaa atattggtga tggtgcaatt attggaacaa gagcagtagt tactaatgac
     5581 gtcccaccgt attctattgt tggaggagta cctgcaaaaa ttataaaaaa gagatttaat
     5641 aatgatacaa tttcaaaatt attagaaatt aaatggtggg attggccata taaaaaaatt
     5701 caatcaaaca ttaagcatat tcaatcagga aatattgata aattagttta atattttata
     5761 atttatttat attaaacttt actttaataa tcattttata agagcttttt caaaatttgt
     5821 atattatttt aatatatat
//
LOCUS       DBGJFF010000348         1335 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B25
            SAMN43225832-rid25700833.guided.303, whole genome shotgun sequence.
ACCESSION   DBGJFF010000348 DBGJFF010000000
VERSION     DBGJFF010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225832
            Sequence Read Archive: SRR30483188
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674350.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674350.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:36:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,060
            CDSs (total)                      :: 3,983
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 8 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 10 of 39
            Pseudo Genes (multiple problems)  :: 5 of 39
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1335
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225832-rid25700833.guided.303"
                     /isolate="B25"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020-01"
                     /collected_by="SSI"
     gene            226..1164
                     /gene="blaCDD"
                     /locus_tag="ACOIAF_004060"
     CDS             226..1164
                     /gene="blaCDD"
                     /locus_tag="ACOIAF_004060"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HGZ2670981.1"
                     /translation="MKRKKNFIWIAILLVGVVMVMYYVGKKHNDINQKTDKNYIKSEL
                     NKSKIENNNKNKKEKINMVDYSDCFEGISGGAIFYNTKNKEYNIYNKELIETRRSPCS
                     TFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYYKKLID
                     KVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQTMAKI
                     FEGDTNFKKEHINILRDIMKIDVNDKNINVYGKTGTGFDEKNKRVDAWFVGMLEREGD
                     TYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
BASE COUNT          567 a          112 c          223 g          433 t
ORIGIN      
        1 tatatcacaa attaaaagga ggacaggatg ttattaatag ttacctaatt atcttcgcaa
       61 taggtttaat caacataatt gtactaggtg aagaaaatat aagagataca gctattcatt
      121 tagtcttgtt tcttataagt agaacctaga ttggagtata ttacaaataa tgtctactat
      181 tttaatagta ggttataaat ttaaatatgg gagatgatat aaattatgaa aagaaaaaag
      241 aattttattt ggatagctat cttattggtt ggtgtagtta tggtaatgta ttatgtaggg
      301 aaaaaacata acgatataaa tcaaaaaact gataagaatt atataaaatc tgaattaaat
      361 aaatccaaaa ttgaaaacaa taataaaaac aaaaaagaaa aaattaatat ggttgactat
      421 agtgattgtt ttgaaggtat tagtggagga gctatttttt ataatactaa aaataaagaa
      481 tataatatat ataacaaaga attaattgaa acaagaaggt caccatgttc tacttttaaa
      541 atagtttcaa ctttaattgg tttagaaaaa ggggtaataa actcaaaaga atcagttatg
      601 ggatatgatg gtacagagta tccaaataaa aattggaata aaaatcttag tttagaagag
      661 gcatttaagg agtcttgtgt ttggtactac aaaaaattga tagataaagt tgatgctaaa
      721 tctgtccaaa atattttaga tgatttaaag tatgggaatt gcgatattag cgaatgggaa
      781 ggagatttaa aaaatggaaa gggacattta aatggatttt ggttagaatc ttcattacaa
      841 atatctccaa aagaacaagt gcaaacaatg gcaaaaatat ttgagggaga tacaaacttt
      901 aaaaaagagc atattaatat tttaagagat attatgaaaa tagatgtaaa tgataaaaac
      961 ataaatgtat atggaaaaac tggaacagga tttgatgaaa aaaataaacg tgtggatgca
     1021 tggtttgttg gtatgctaga gcgtgaaggt gacacctatt attttgctat taaatcagat
     1081 gattccaata aagaaattac agggccaaag gtaaaggaaa ttgcaattaa tataattaag
     1141 aaatattata gtgtaaggga ataaacttaa ttttatttgt aaatattatt tacctatact
     1201 ctgtaatatt tattaaataa ttttactaaa ttgagtctat atagagcaaa actcctttag
     1261 tacataaaat acttgctatt gattattaaa ttaaatacct atagatttaa agttaatacc
     1321 ttttaaagac tatgt
//
